LOCUS       DATIAL010000001         3690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_322587, whole genome shotgun sequence.
ACCESSION   DATIAL010000001 DATIAL010000000
VERSION     DATIAL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322587"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..893)
                     /locus_tag="VLA60_00005"
     CDS             complement(429..893)
                     /locus_tag="VLA60_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSF07759.1"
                     /translation="MGVHEALSRLGLKLPAAPCPVGSYVPARQAGDLIFVSGVLPFRD
                     GLIVHPGKLGRELTVEEGAAAAQLAMLNGLAILQEMMGDLASLKQVVRLTGHVASVEG
                     FVDQPAVINGASDLLVKLFGDAGRHARLALGAFELPLHAPIELELIVQVSPP"
     gene            complement(918..1925)
                     /gene="mltG"
                     /locus_tag="VLA60_00010"
     CDS             complement(918..1925)
                     /gene="mltG"
                     /locus_tag="VLA60_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HSF07760.1"
                     /translation="MILVRRTVVSLLTVVVLVMGGFVWMNQPMGGSTQPVLVEIPPGT
                     PFTQVSHILDHHHLIASEWFFTLLGRVQRVDRNIIPGEYELDAGMQPTELLHKLVKGE
                     VYQYAITIPEGYTVAQIADILDQKRLALKQDIFRLSHDPVFIQSLNIQAPTLEGYLFP
                     DTYHFSRFTPPESIIRTFVHRFHDVMIPELKDRAMVMGMTLQEVLTLASVVEKETGLA
                     AERPLVSGVFHNRLRLGIPLQSDPTVIYALESFDGNIRKADLSVDSPYNTYKVRGLPP
                     GPIANPGLAAIHAALYPTTTDFVYFVARNDGSHQFSVTLADHNKAVDLYQRRSISKRA
                     S"
     gene            complement(2006..>3690)
                     /gene="alaS"
                     /locus_tag="VLA60_00015"
     CDS             complement(2006..>3690)
                     /gene="alaS"
                     /locus_tag="VLA60_00015"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HSF07761.1"
                     /translation="LYDLVSSVVDHMDSVYPDLRSMKDTVSEIVQGEETRFIGTLEQA
                     LPLLNQILEETKQRGSQILEGEAVFKLYDTYGFPLDLVEDAAREEGLAVDHIGYQQAL
                     EAQRDRARKTARFSQADSRSDLVNALGQFPLTKFVGYASQEDRGKLLALVKDEHVIKE
                     AKQGETVECVLDTTPFYPEGGGQVGDQGILVGPSARILVQDTTKLAKGWFLHKGQVIE
                     GSVHVGDVLTATVQGHLRQSAARNHTATHLLHAALREILGPHVKQHGSLVAPNRLRFD
                     FSHFKGLTPRNIEEIEGLVNEQIRQNLSVKVEEMGIQDALSRGALAFFGDKYGEQVRV
                     VEMGSFSQELCGGTHCARTGDVGLFRIVSEGGIAAGVRRIEALTGEGAVAQTQHQEAE
                     WRELAAVLKAGPNEVVEKVKKLVGTLRDTERELERAKQKLLDQQGAGQEASIRDIGGM
                     PILIQRIDGLTIQELRTFSDKLRHKVPSGLLVLGSVKEGKVSLLVIVAKEQAHKIPAG
                     KVAQHVAQLVGGSGGGRPDMAQAGGNQPEQLDEALRSVYNYIASHIGTLKEE"
BASE COUNT          989 a          990 c          827 g          884 t
ORIGIN      
        1 ggggatggaa ccaagtcaga gcgggggtcc agtatgacta tatcaagcga tcttttcatg
       61 cgtcaattta gaagaactgc acagacagaa agatgaacat tcacagccat ttcgtattat
      121 agattttcag gaaaactctc cgtttcccca ttgccccttg accaaatggg tgaaaaaaag
      181 tcccacaccg catatgtaaa agatctccgg acacttctaa tggggatctt ccgctttgaa
      241 tggggatctt ccgctttgaa caaacggaaa tgaaagtagc atcgctgaaa caaattcaga
      301 tatcgagcca tagcaacaac gcctgaaatc ccaaataaag cggctagagg acaagagaag
      361 tgaatcctta tgcaaagcag gagttgtttt gcacatgctg aagggataat tacggagtaa
      421 gaaccggctt aagggggaga tacttgaacg atcaattcaa gctcaattgg agcatgaaga
      481 ggtaattcga aagccccaag agccaatcgg gcatgacggc cggcatctcc aaacagcttg
      541 accaacaaat cagaagcccc gttaatcacc gcaggctgat ccacaaaacc ttccacggac
      601 gctacatgcc ccgtcaaacg caccacctgt ttgagagacg cgagatctcc catcatctct
      661 tgaaggatgg ctaacccgtt tagcatggct aactgggcag cagcagctcc ttcttctacc
      721 gtcagctctc gcccgagttt tccagggtgc acaattagcc cgtcacgaaa aggcaacacc
      781 ccactcacaa agattaagtc tcctgcctga cgagccggaa catatgaccc gacaggacaa
      841 ggtgccgcag gcagctttaa tcccagtcgg cttaatgctt catgaacacc cataacggat
      901 attacacaat cactcactta cgaagcacgt ttactaatcg atcgtcgttg ataaaggtcg
      961 accgccttat tatgatccgc taatgtcaca gaaaattgat gactcccatc gttccgtgcc
     1021 acaaaataga caaaatcggt tgtcgtagga tagagcgcag catgaattgc ggccaaaccc
     1081 ggattagcaa tgggtccagg aggaagcccg cgtaccttgt aggtgttgta tggactatct
     1141 accgatagat cggctttccg aatatttcca tcaaacgatt cgagggcgta aatgacggtg
     1201 ggatcgctct gcaagggaat gcccagccga aggcgattat gaaatacgcc cgaaaccaag
     1261 ggccgttcgg ctgccagccc cgtttccttt tccaccacgg aagctagggt caacacctcc
     1321 tgaagagtca tgcccatgac cattgcccgg tctttgagtt ctggaatcat cacatcgtga
     1381 aaccgatgaa caaatgtgcg aataatggat tctgggggag taaagcgaga aaaatggtag
     1441 gtatcaggaa aaagataacc ctctaatgta ggggcctgga tattcaggct ctgaataaat
     1501 accgggtcat ggcttaatcg aaaaatatcc tgcttgaggg caagacgttt ttgatccagt
     1561 atgtccgcaa tttgtgcaac ggtatagcct tcgggaatgg tgatggcata ttgatagact
     1621 tcccctttaa caagtttgtg cagtaattcg gtgggttgca ttcccgcatc cagttcatat
     1681 tcgcctggaa tgatatttcg atcgacccgc tgcacacgcc ccaaaagagt aaaaaaccat
     1741 tctgatgcaa taaggtgatg gtgatcgaga atgtgggaaa cctgcgtaaa aggagtaccg
     1801 ggtggaattt cgacaagaac cggttgggtt gatccaccca tgggttgatt catccacaca
     1861 aaaccaccca tgaccagaac taccaccgtt aaaagactta ctacggtccg gcgaacaagg
     1921 atcacgtcac gacgtcccct ttcctactgc tccccgtcgg tccatgtggg gggacaatgc
     1981 agccacatca tcatgctgag ggagtctact cctccttcaa tgtacctatg tgtgaggcaa
     2041 tatagttata tacgctccgt aaggcctcat ctaactgttc tggttgattt ccaccagcct
     2101 gagccatatc tggtcggccg cctccagacc ccccaacaag ttgagccaca tgttgagcga
     2161 cttttcctgc agggattttg tgagcctgtt ctttggccac aataaccaat aacgaaactt
     2221 ttccttcttt gaccgacccc aaaaccaaaa gaccggaagg cactttgtgg cggagcttat
     2281 cagagaaggt tcgcaattct tggatagtca acccatcgat tcgctggata aggatcggca
     2341 tcccgcctat gtcccgaatc gacgcttcct gaccagcacc ttgctgatca agcaattttt
     2401 gtttcgcacg ctctaattcg cgttcggtat cacggagggt tccaacaagt ttcttgactt
     2461 tttcaacgac ttcattgggg ccggctttca gcacggcggc caattctctc cattcggcct
     2521 cctgatgctg agtttgagcc acagccccct cccccgtgag tgcctcaata cgccggacac
     2581 ctgccgcaat acctccttcg gacacgatac gaaacagacc gacatctccg gttcgagcac
     2641 aatgggttcc gccgcacaat tcctgactaa atgaacccat ttctacgacc cgaacctgtt
     2701 caccatactt atcaccaaaa aaagccagag ctcctcgact cagagcatcc tgaattccca
     2761 tttcttcaac cttaacagat aggttctgac ggatttgttc gttcaccagt ccttcgattt
     2821 cttcaatatt tctgggggtc aatcccttga aatgtgagaa atcaaaccgc aatcgattgg
     2881 gagccaccaa agatccatgt tgcttcacat gaggacctaa aatttcccgc aatgcggcat
     2941 gaagaaggtg agtggccgta tgatttcgtg ccgcactctg ccttaaatga ccctgtaccg
     3001 tggcggtgag aacatcgcca acgtgaacag atccttcgat gacctgaccc ttgtggagaa
     3061 accacccctt tgccagcttt gtggtatctt gaacgagaat tcttgccgac gggcccacca
     3121 agattccttg atccccgact tgtccacctc cttcgggata aaaaggcgtg gtatcgagga
     3181 cacactccac cgtctcccct tgcttggctt ccttgatgac atgctcgtcc ttcaccaagg
     3241 cgagtagctt ccctctatct tcctgactgg cataacccac aaatttagtc aaagggaact
     3301 gcccgagagc attcaccagg tcgcttcgag aatccgcctg gctaaacctc gcagtttttc
     3361 tggctcgatc acgttgcgcc tctagcgctt gctgataacc gatatgatcg acggccaacc
     3421 cttcttctct tgcagcatct tccaccaaat caaggggaaa cccgtaggta tcatacaact
     3481 tgaaaaccgc ttctccttct aagatttggc tccccctttg cttggtttcc tccaggattt
     3541 gattgaggag aggcaaggcc tgttctaagg ttccaataaa gcgtgtttcc tccccctgga
     3601 caatctcaga aaccgtatct ttcatcgaac gaagatccgg atagaccgaa tccatgtgat
     3661 caaccacgga ggatacgagg tcatataaaa
//
LOCUS       DATIAL010000002         5237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1397936, whole genome shotgun sequence.
ACCESSION   DATIAL010000002 DATIAL010000000
VERSION     DATIAL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397936"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VLA60_00020"
     CDS             complement(<1..1110)
                     /locus_tag="VLA60_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07762.1"
                     /translation="MTSFPPKMFQVSVLPYAVAIFLSAFLLFQVEPIIARYILPWFGG
                     TPAVWTTCMLFFQVFLLVGYAYAHLLASYLSPRHQALVHLGLVVCSLVFLPITPGDGW
                     KPDGTQNPMLAIILLLMVTIGVPFLLISASGPLLQHWFNRVHPTVSPYRLFALSNLGS
                     LLGLVSYPFFIEPQLGLRTQTLFWSAGYGLYAVMCAWGARPLFRLATRSGFSENSEYR
                     PDTKPRRPESLLTLALAACGSVVLLASTNQICRDIAVIPFLWVLPLGLYLISFILCFD
                     HPRWYDRRIWVPVFLVLLSAVVYLLHQEYAETEINLYLQIFIYSAVLFACCMVCHGEL
                     ARLKPPPRFLTSFYLMVALGGALGGVFVNLVAPVLF"
     gene            complement(1521..1844)
                     /locus_tag="VLA60_00025"
     CDS             complement(1521..1844)
                     /locus_tag="VLA60_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSF07763.1"
                     /translation="MGSKLYVGGLPYSATQAELTDLFSAHGTVESANVISDKFTGQSR
                     GFGFVEMSSGEEAQAAISALNSTEFGGRTLTVNEAKPQAPRTGGGGYGGGGGDFGGDK
                     RRSRF"
     gene            complement(2511..3488)
                     /locus_tag="VLA60_00030"
     CDS             complement(2511..3488)
                     /locus_tag="VLA60_00030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HSF07764.1"
                     /translation="MPKIIPGPNIEVVLPPNLNHIYFADASQHPFRFRSQSFQLVNAW
                     WLAEAALLAYAEREFAIPQYTKAGLTVEGKQPFSIGGSTQCYVAHNQDVVIVAFRGTQ
                     VLKPVGGKLPGEMWRQVVKDLWTDGKFRLIVSGQGGSVHEGFKMALDEVWEPLKSYLD
                     RLKGEKPARTFWFTGHSLGAALATLAADRYGDVQGLYTFGSPLVGDEGFARDFYVSGY
                     RFVNNNDVVARIPLWGPNALNLMKWGRYEHVGLLKYIDETGRLFDNPSMLERVQRGVG
                     GQVQLLREVMNQWSNGEFSDIPIDCFNDHAPLYYALRIWNCYEQELQGV"
     gene            complement(4035..4661)
                     /locus_tag="VLA60_00035"
     CDS             complement(4035..4661)
                     /locus_tag="VLA60_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07765.1"
                     /translation="MPAMYQIFGQTAALIMLLVSSGCSDSSTDPASQEPKVDQSGFTA
                     SLSGSVSGEVSGAGVVTYLPPKEGDPVTGVRPGYFLVANLNSDRNDKREFLIILRVPG
                     EAQPGHYDLVAPDPLRVGENFDVQVEMVEGGRSISYQSNTEGTITLEDFAPYSAGSGD
                     GNITGTFQFVAENSEGGQISATGTFDLPLGRRVVFQDSAYSGKDVSKG"
     gene            complement(4778..5185)
                     /locus_tag="VLA60_00040"
     CDS             complement(4778..5185)
                     /locus_tag="VLA60_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4168 domain-containing protein"
                     /protein_id="HSF07766.1"
                     /translation="MNSMATSLQKLSVLLVIPSLCGLGLLSVVHAQNINAQPQKVESQ
                     PMQQELIGPNLEPFAGAYKEISQIHTTYKERIIQAGDPTKSEALQEEANRKMSQAVTD
                     HGLTIKDYNTIFQSIQNDPALKKEFMTVLNRTQ"
BASE COUNT         1426 a         1347 c         1252 g         1212 t
ORIGIN      
        1 aaagagaacg ggtgcgacca ggttgacaaa caccccaccc aaggctcccc ccaacgcgac
       61 catgagatag aaggatgtga gaaatctggg gggaggcttc aggcgagcaa gttccccgtg
      121 gcacaccatg cagcaggcaa agaggacggc cgaatagatg aaaatctgga ggtagagatt
      181 aatctccgtc tccgcatatt cctggtgcag caggtagacg actgcggaga gcaataccag
      241 aaacacaggc acccagatcc gtcggtcgta ccatcgcggg tgatcgaaac acagaatgaa
      301 ggagattagg tagagaccca gcgggaggac ccaaagaaac gggatgaccg cgatatcccg
      361 gcaaatctga ttcgtgcttg caaggaggac cactgatccg caagcagcga gggcgagggt
      421 caggagactc tcaggccgac gcggcttcgt gtctggtcga tactcagaat tttctgagaa
      481 tccactcctc gtggccaacc gaaatagcgg tctggcgccc caggcacaca tgaccgcgta
      541 caggccgtac ccggccgacc agaagagggt ctgcgtgcgc aaccccagtt gcggctcgat
      601 aaaaaaagga taggagacca atcccaagag tgagcccaga ttcgataatg cgaaaagccg
      661 atagggtgac acagtgggat ggactcgatt gaaccaatgt tgcaggagag ggccagaggc
      721 cgagatgagg agaaacggta ccccaatggt caccatgaga aggagaataa tcgccagcat
      781 cggattttgt gtgccatcag gcttccatcc atcccctggg gtgataggga gaaaaaccag
      841 agaacacacc accagaccca ggtgaaccag ggcctggtgc cgaggggaga ggtagcttgc
      901 gagaagatgg gcataggcat acccgaccag aagaaagacc tgaaagaaga gcatgcacgt
      961 cgtccagacc gccggggtgc ctccaaacca gggaagaata taccgggcga tgatgggctc
     1021 tacttgaaac agcagaaagg cactgagaaa gatcgcaacg gcatacggaa ggacagacac
     1081 ctggaacatt tttggtggga aggaagtcat ggccttcatg ttgacaggcg gtggggatgg
     1141 ggcattgttc atacacgcag ccgattttgc atggtcattg caggattggt actaccctgg
     1201 atctcacact gccgggtatc aactcttcat tcggttcaaa tccatgcacc cgatccaccc
     1261 tgtaacgcgt tgagcatcga gccggtgtgc cccgtgagac cggaaccaat actctatctg
     1321 cgtgctccgc caagccctat cctataaaac aaatggctaa aagtcgggca tcatactatc
     1381 ctttttctag acaatccacg ctccacaggt ccaatgtggc acatttggat taggccaaac
     1441 attccaactg ctgcatattg acaaaaaaag ggagagatca tattgatctc tcccttttct
     1501 gaaacgtatt ggtgttctgg ttagaaccgg ctgcgacgtt tatcgccacc aaaatctcca
     1561 ccgccaccac cgtatcctcc accacccgtg cgtggagctt ggggtttagc ttcgttgacc
     1621 gtcaatgtgc ggccaccaaa ctcggtagag ttcagggctg aaatcgcggc ttgcgcttcc
     1681 tctcccgaag acatttcgac aaatccgaac ccgcgcgatt ggccggtgaa cttgtcgctg
     1741 atgacattgg ctgactcgac ggtgccgtgt gccgaaaaca agtcggttaa ttctgcttga
     1801 gtcgcggaat acggcaaacc acctacatac aattttgaac ccatgggggt cctcctttta
     1861 aaaaatgata ttgtcttagg ctcgagaaag ggtaaaacct ggagaaggag taggccgcgg
     1921 acgctgcatt catgcgacag tggacagaca ctttcgagtt atctctcggg caaaacaaca
     1981 ccggttacgg gctttggtaa tttgattgct catcgccaag ccctaggcgt taagttcttt
     2041 caaactagca caagagaagg gaagaagata gccaattcca gtaagaggga acggaaaatt
     2101 ttgattgaaa tatgggcctg gctttgagca attgaggcga gaatgtcttt ttctttagca
     2161 ttttcagcat tctacgagaa agccaaccct tcctggcagg aaagtgcagt gtggaaatac
     2221 gccagaaata cagtatccaa accggaattt cccagcggct tccatgctga tttttaccgg
     2281 aattccgaaa cggtcaggct gttcccagcc agatggacgg atttcgcctc agctaccatg
     2341 gcttgaatgc cgatttgtcg ttgtgaagag aggggggatt tgttttattt tttaacgaga
     2401 cgaggggata aatattcaac aggagtggct gacccattca attggctttc catgatggaa
     2461 tgaagtcaca gataccgcat tcacattaga aacctggggg aaatctactg ctagacacct
     2521 tggagctcct gctcataaca attccaaata cgaagggcat aatacagggg agcgtgatca
     2581 ttgaaacaat caatgggtat gtcactaaac tcgccattgg accattgatt catcacctca
     2641 cgaagaagtt gaacctggcc tcccacacca cgctgtaccc tttccaacat ggagggatta
     2701 tcgaaaagcc taccagtttc atcgatgtat ttaaggagac cgacatgttc ataacgaccc
     2761 cacttcatca ggtttaacgc attgggaccc cagagtggta ttcgagcgac cacatcgtta
     2821 ttgttcacaa atcgataccc actgacataa aaatccctcg caaagccctc atcccctacc
     2881 agcggagacc caaaggtgta gagcccttgc acatccccat aacggtcggc agctaatgtc
     2941 gccagagccg caccaagact atggccggta aaccaaaaag tgcgagccgg cttttcccct
     3001 ttcaatctat ctaaatacga cttgaggggt tcccacactt cgtccaacgc cattttaaag
     3061 ccctcatgca cagacccacc ctgaccagat acgattaacc gaaattttcc gtccgtccac
     3121 aaatccttga cgacctgacg ccacatctca ccagggagtt tacctcctac cggtttcagc
     3181 acctgcgtgc cgcgaaaggc cacaatcaca acatcctgat tgtgtgccac atagcattgg
     3241 gtacttccac caatgctaaa aggctgcttg ccttccactg tcaagccggc ctttgtgtat
     3301 tgaggaatag caaattccct ttcggcatac gccaacaagg cggcctccgc caaccaccag
     3361 gcattcacca gttgaaagct ttgagaccta aacctgaacg gatgttggct ggcgtcggca
     3421 aaatatatat ggttcaaatt tggaggtaac accacctcta tatttggacc aggaataatc
     3481 tttggcatag gcttagactc cttatgaact gtacgtatgt tcccctgtga ttggaaacag
     3541 ggccattgcc acagaatgat tccgaacgat gacgtttacg atttgcccgt agcaggcatt
     3601 gatggagggg aagtcgcact ctaccgtgca tcctttaagg agaagaggga acgctccttg
     3661 aagtttgacc acagacatcc tgattactgc attcacagga ggactagaaa attttggatt
     3721 actcctcttt cccgctactt gtggcgcgag tttttcttta aactgtgcca acattgtcaa
     3781 ttaaaagcta gacatgaaac gagtaggaat catggatatg agagaccggt cacaacgaga
     3841 atatgtcatt catcaacagg acaaggaacg gggtcggtca caaaaggttt agtggttaaa
     3901 cggaatggcg atcgaacact attcttcagt cgcttaggag gaacttaacc aaagatacac
     3961 ttcagaccca tgctggccct caacatacaa gtccgtctcc taatttttta aacaggccac
     4021 aatgcggcat tttctcagcc cttcgacaca tccttcccgg aataggctga atcctgaaac
     4081 acgaccctcc tacccaaagg caaatcaaac gtgcccgtgg ctgaaatctg ccccccttcg
     4141 ctgttttccg ccacaaattg aaatgttccg gtaatattac catcaccgga accagcacta
     4201 taaggagcga aatcctctaa agtaatggtc ccctcggtat tggattggta tgaaatggat
     4261 cttcctcctt ctaccatttc aacctgcaca tcaaaatttt cacccaccct caaaggatcg
     4321 ggtgccacca aatcataatg gccaggctgg gcctcaccag ggactcgaag aatgatgaga
     4381 aactctcttt tgtcattcct atctgaattg agattcgcaa ccaaaaaata gccgggacgc
     4441 actccagtca cggggtcccc ttccttgggg ggcagataag tcaccacacc tgcccccgac
     4501 acttcaccac tcacagatcc ggataggctt gcggtaaatc ctgactgatc caccttaggc
     4561 tcttgggaag cagggtcagt ggatgagtcc gaacatccac tgctgacaag caacataatc
     4621 aaggccgctg tttgtccaaa aatctgatac attgctggca tgtgctccct cccatctgaa
     4681 tggacccttt tgcctctccc actctgagaa accgggtttt tgtcgtttaa gacattaatc
     4741 tttatcaaaa aatcgagtag agcggggatt cattccttta ctgcgttcga ttcagtaccg
     4801 tcatgaattc tttcttgagg gccgggtcat tttgaattga ttgaaatatt gtattgtagt
     4861 ccttgatggt caatccatga tccgtcacgg cctgactcat cttccggtta gcctcttctt
     4921 gaagagcttc acttttggtt gggtcacctg cctggatgat acgctctttg tatgtcgtat
     4981 ggatctggga gatttccttg taggctccag caaagggttc aagattcggc ccaatcaatt
     5041 cctgctgcat aggttgggat tcaactttct gaggttgggc attaatattt tgcgcatgta
     5101 ccacagaaag aagtcccaag ccacatagac tcggtatcac tagcagcaca ctcaatttct
     5161 gtaaactcgt tgccatggaa ttcataccag ctctcctttg ttagtttgaa taaatattcc
     5221 ccagcaagct gagatcg
//
LOCUS       DATIAL010000003         5053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_968170, whole genome shotgun sequence.
ACCESSION   DATIAL010000003 DATIAL010000000
VERSION     DATIAL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968170"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..720
                     /locus_tag="VLA60_00045"
     CDS             379..720
                     /locus_tag="VLA60_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HSF07767.1"
                     /translation="MKQCVNLVLVGLMCFMLVGCGAASFQQPNASFAVAGIYTDVQGG
                     SLVLDNGVAAAKTGTACSHQILGIVASGDTRVETAMANGGISKLVYVNTSYKNIVWGV
                     YTEVCTIARGN"
     gene            845..1477
                     /locus_tag="VLA60_00050"
     CDS             845..1477
                     /locus_tag="VLA60_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSF07768.1"
                     /translation="MKILAVLICLMLLSAPSKVYALENIEIYLAGYGMATQPLSQDLS
                     FKGNGVTDERIHGHPGLGVKVGLFPSFFKGYLGVELESFGQNNSLKFPIEENGAETGE
                     GKSGLVTYSSMVNVILRYPGLYARPYVGMGGGLSNGILHHSDIPGRKDRNMETSLAPG
                     YQFFGGIQFVVAANWFVFGEYKYSAANYHWNQLSVNFRSEYFIGGIGYIF"
     gene            complement(1594..3495)
                     /locus_tag="VLA60_00055"
     CDS             complement(1594..3495)
                     /locus_tag="VLA60_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HSF07769.1"
                     /translation="MVNILLFLVLLLLPFELKAQAPFSPYLQELLQQATTEKLYQDRY
                     WHRLLHYRQNPLGGHTSEVDDPGFFLSPDGKTDPEAELQATLTYFFSDTLVGRSKQPA
                     QCAFIARYHWLKEKLHFQESKLLPHPCERFVSWLKELNPASITFIFPSAYMDNPASMF
                     GHSFLRVDQKGQTAETNILAYTINYAAEVPPDAGIEFAYKGIFGGYKGFFSTIPYYMK
                     VKEYRDLENRDIWEYRLNFSQEQYHRLLMHAWELGNAYFDYYFFKENCAYHILSLLEV
                     ADPNLHLTDQFHFATIPADTIRLLTRQKELVQKISYRPARSTLLKRKREALTKEEAAL
                     VRKLLHDPQIISQSEFLQLPQNRKTFLLDFASDVLRYKGVTDQDNVEAYKAKNRSILV
                     TRSHIPIPSVPFPVKPFSLSPEHGHQTIRVGGGVGWRNNKIFEEINFRGAYHDLLDPD
                     PGYTQDAQIELLDLRLRHYQHQNQFRVERFTFANILSLAPIDSLFISPSWKINVGMNT
                     VKTSTCDLCSNGNANAGIGGALETRVFQREVFFLFGEMDANISGGYKENYRVGGGVSG
                     GVMATITPNWKWLMSAGYLYYPLGDRSHDIPISVGQRWTFAKNFAVRATFTHHDQDNE
                     IFGVFHGYF"
     gene            complement(3555..4073)
                     /locus_tag="VLA60_00060"
     CDS             complement(3555..4073)
                     /locus_tag="VLA60_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 domain-containing protein"
                     /protein_id="HSF07770.1"
                     /translation="MAEKPWVLVSTFFLVMGMSGMAFGNPPNNGPGCGLGKLAWEETS
                     VSKSNIAPQVMMATTNGTFGSQTFGISSGTSGCTNDGQVWASEKVNVFAAINFDNLAQ
                     EMAQGEGEHLTSLATLMGIPVAQQPAFFAMTQEKYTTLVQEGETSPKAVVKAIHDAMV
                     DHPVLAQVTTSK"
     gene            complement(4239..>5053)
                     /locus_tag="VLA60_00065"
     CDS             complement(4239..>5053)
                     /locus_tag="VLA60_00065"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF07771.1"
                     /translation="QWLADVPHVHTYETSGVDLREFPHSSFDLVYSYVTFQHMPRPVF
                     ERYLGEIHRVLTPHGYLVFQLPIGHYLDVPLEDTIGIRSYSHQEIEETLRRNGLGSLP
                     NPSSNPEFTETSETHSHRFRLAQKIGPLRPTVSMNWDELEQQHFVYELENHLYGMYAD
                     HCASAGNYQEGIQTLQTLINKNPATLEGRLRLAALLIETGQLPQALVTMKEIITLHPG
                     YQEGHRTLRQLLEKRPNLHPSQNPSLSTTRKCSPAHTSRNTPALANREYSTT"
BASE COUNT         1302 a          999 c         1310 g         1442 t
ORIGIN      
        1 tttcgccaat tccgccaggg tctcaattcc cagcccttgt ccaaagcggg ctttttttat
       61 gatatcgcaa aattcgtctt ctaaactcat ttacatgctc atatgaaatt tggtcataag
      121 aatcggaagc tctcttttct gcacacgtcc ttcatctaaa caaaaaagtg gggtctgtgg
      181 aaagggggtg tcccagattt taacccagtg agattcatcg tatagaaagt ttctatctat
      241 aacttttttt gaagagaaag tgcgtttctt gtagaagcag gagataggat tttgttgaca
      301 ttattttttg aatacgctac ttaagggtaa cagaattttt ggtgaaagtt tcatatacca
      361 ttaacagagg aggttttgat gaaacagtgc gtaaatctgg tccttgtggg attaatgtgt
      421 ttcatgcttg ttgggtgtgg ggcggcaagt tttcaacagc cgaatgcgtc atttgctgtt
      481 gcgggtattt acactgatgt gcaggggggg agcttggtgt tggataacgg tgtcgcagcg
      541 gcgaaaactg gaacagcttg tagccaccaa attttgggca ttgtggcctc tggagataca
      601 cgagttgaga ccgccatggc caatggagga atttcaaagc tggtatatgt gaatacctcc
      661 tacaaaaata ttgtatgggg agtgtatact gaagtgtgta cgattgcgag aggaaactag
      721 gtttcccaaa gagaaattcc catgttggcg gggagcacct ggaaggttat tctgggtgct
      781 cccttttttt tgcaaaaatg accgggcctg ggtttttgcg gagtgaaaat ttggaacgtt
      841 cagcatgaaa atcctagcag tcctgatatg cttgatgctg ctttctgctc catcaaaagt
      901 atatgcattg gagaacatag agatttatct ggccggttat gggatggcca ctcaaccctt
      961 aagccaggac ctttcattta aggggaatgg tgttactgat gaaagaattc atggtcatcc
     1021 tgggctggga gtgaaggttg gattattccc gtcttttttt aagggatatc ttggagttga
     1081 actggagtca tttggacaga ataactcctt gaaattcccc atcgaagaaa atggtgcaga
     1141 aacgggtgaa ggtaaaagcg gccttgtgac gtatagctcg atggtcaatg tcatcttgcg
     1201 gtatcctggc ctatatgcac ggccgtatgt gggaatggga ggggggctct cgaatggaat
     1261 ccttcatcat tcggatattc ccggtagaaa ggaccgcaat atggaaacga gtttagctcc
     1321 gggatatcag ttctttggcg ggattcagtt tgtagttgcc gcgaattggt tcgtgtttgg
     1381 ggagtataaa tacagtgctg cgaattacca ctggaaccaa ctttccgtga attttcgaag
     1441 cgaatatttt ataggtggaa ttggttatat attttaatgt ggcgttcaac ttgtctctgt
     1501 aactctcatg ctttcgcacc tggtttaagt ggttctgtgc cgttgtttgg agctgtggat
     1561 cgtatagagg gtgaaaataa tggcgttcct gaatcaaaaa taaccatgaa atacaccaaa
     1621 aatttcattg tcctgatcat ggtgggtgaa ggtggcgcgc accgcgaagt ttttggcaaa
     1681 tgtccatcgt tggcccacag aaatagggat gtcatgtgat cgatctccaa gggggtaata
     1741 caaatatcct gctgacatga gccatttcca gttcggagtg atcgtggcca tcacgccgcc
     1801 agatacccct cctcccaccc ggtaattttc tttgtacccg ccactgatgt tggcatccat
     1861 ctccccaaag agaaaaaaga cttcccgttg aaacacccga gtttccaggg ctccaccgat
     1921 tcctgcattg gcattcccat ttgaacagag gtcgcaagtg gatgtcttga cggtgttcat
     1981 tccaacattg attttccatg atggcgaaat aaacaacgaa tcaatggggg ccaaggagag
     2041 gatattggcg aaggtgaacc gttccactcg aaattggttt tggtgctgat aatgcctcaa
     2101 ccgcaaatca agtaattcta tttgggcatc ctgggtgtat cctgggtcag ggtccaggag
     2161 atcatgatag gcgcctcgga agttgatttc ttcaaatatt ttattgtttc tccatccgac
     2221 tccacctccg actctgatcg tttggtgtcc gtgttcggga gaaagggaaa atggtttcac
     2281 cggaaagggg acggatggga tgggtatgtg actgcgtgtc actagaatag atcgattttt
     2341 ggccttatag gcttcaacgt tgtcttgatc ggttacccct ttatagcgca agacgtctga
     2401 agcgaaatcc agaaggaatg ttttgcggtt ttgtggtaat tgaagaaatt cggactggct
     2461 tattatctga gggtcgtgaa ggagttttct gaccaaggcg gcttcttctt ttgttaatgc
     2521 ttctctcttg cgtttgagga gagtactccg ggcgggacga taagaaattt tttgaaccaa
     2581 ttctttctgt cgagtaagaa ggcgaatggt atcggcagga atcgtcgcaa agtgaaattg
     2641 gtcggtcagg tggagattgg gatcggctac ttccaggagt gagagaatgt ggtacgcaca
     2701 attttcttta aagaagtaat agtcaaaata ggcattccct aattcccacg catgcatgag
     2761 caatctgtga tattgttctt gtgaaaaatt caagcgatat tcccaaatgt ctctattctc
     2821 gagatcccgg tattctttga ccttcatgta gtagggaatg gtcgaaaaga accccttgta
     2881 tcctccaaat attcctttat atgcgaattc aattcccgca tccggcggga cctccgcagc
     2941 gtagttaatt gtatacgcca aaatattcgt ttctgcggtt tggccttttt gatccactcg
     3001 taaaaatgaa tgtccgaaca tggaggccgg gttatccata taggccgaag gaaaaataaa
     3061 ggtaatcgag gcaggattca gttctttaag ccaactgaca aaacgttcac atggatgtgg
     3121 aagaagctta gattcctgaa agtgcagttt ctctttcagc cagtgatacc gggcaataaa
     3181 agcacattgt gccggctgtt tggagcgacc aactaaggtg tcagaaaaaa aatatgtcag
     3241 tgtggcttgt aattcagctt ctggatcggt tttcccatct ggtgaaagga aaaaccctgg
     3301 atcatcgacc tcgctggtgt ggccacccaa agggttttgt cggtagtgga gtaaacgatg
     3361 ccagtagcgg tcctggtaaa gtttttccgt ggtggcttgt tgaaggagtt cttgcagata
     3421 gggagaaaaa ggggcttggg cctttagctc aaagggaaga agcagaagta ctagaaaaag
     3481 aagaatgttt accacatgtc aggaaggtaa agaaaagaga ggctccggct ttaccagccg
     3541 gagcctgaaa taatttactt cgatgtcgtg acttgagcta aaacgggatg gtcgaccatg
     3601 gcatcgtgaa tggcttttac caccgccttt ggcgaggttt caccctcctg aaccagagtg
     3661 gtgtatttct cttgggtcat ggcaaagaac gcaggctgtt gggcgacagg aatacccatt
     3721 agtgtggcca atgacgtgag atgttcgcct tctccttgag ccatttcttg ggccaaattg
     3781 tcaaaattaa tagccgcgaa gacattcact ttttctgatg cccagacttg cccatcattg
     3841 gtgcaaccgg aagttcccga actaattcca aatgtttgag atccaaaggt gccgttggtt
     3901 gtggccatca tgacctgagg agcaatattg cttttactga cagaggtttc ttcccacgct
     3961 aatttcccta acccgcaccc tgggccattg tttggcggat tcccaaatgc catgcccgac
     4021 attcccatga ccagaaaaaa tgttgacacc aatacccatg gtttttctgc catgctttcc
     4081 tcctagtttg gtagtgagag ataaagtata gatttgcgac ccgtgaacat gattataaca
     4141 aaagaaaggt tatttgaaag ccaaaaatta cacatgcatg ttgatcacct atggttggct
     4201 gacccgaggc acggtggata gtgaattgtg ttcagacttt atgtggttga atattccctg
     4261 tttgccaatg caggagtatt tcgggatgtg tgggcaggag agcacttcct ggttgtggat
     4321 agagaaggat tctgggaagg atggagattc ggacgtttct cgagaagttg cctgagcgtt
     4381 cgatggcctt cttgatatcc aggatggagg gtgatgattt ctttcatggt aaccaaagct
     4441 tgtgggagtt gtccggtctc gatcaataag gctgctaacc gtaatcttcc ttccagagtt
     4501 gcgggatttt tattgatcag ggtttgcagc gtctgaattc cttcttgata gttgcctgca
     4561 ctcgcacaat ggtcggcata catgccataa agatggtttt ctagttcata gacaaagtgc
     4621 tgttgttcca attcgtccca gttcattgag acagtgggcc ttaatggacc gattttttgt
     4681 gccaaccgga aacggtgact atgcgtttca gacgtctctg tgaattcggg attggatgat
     4741 gggttcggaa gggagcctaa gccgttccgc cgaagggttt cttcaatttc ctgatgggaa
     4801 tacgaccgta tcccaatggt gtcctcgaga ggaacatcaa gataatggcc tatgggcagt
     4861 tgaaagacca gatagccatg aggagtcaag acgcggtgga tttctcccag gtaacgttca
     4921 aacacaggac ggggcatatg ctgaaatgtg acataggaat agaccagatc aaatgaggag
     4981 tgaggaaatt ctcgaagatc cactccagag gtttcatacg tgtgaacatg gggaacgtcg
     5041 gctaaccact gtt
//
LOCUS       DATIAL010000004        20402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_430526, whole genome shotgun sequence.
ACCESSION   DATIAL010000004 DATIAL010000000
VERSION     DATIAL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 20402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20402
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430526"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VLA60_00070"
     CDS             complement(<1..855)
                     /locus_tag="VLA60_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF07772.1"
                     /translation="MGKTILVTGAAGFIGSHAVEALVRRGDQVIGLDNLNDYYDPVRK
                     EKNLGEVTAHAKTGKWPGTFQFLKGDIRDRELVAHLFSDHRFDAVVHLAAMAGVRVSI
                     DDPALYYDVNVMGTMALLDACVGRIGSQGKHSPSPTFVFASTSSVYGNTKTIPFVPED
                     SCDKPLAPYAASKRASELLGYTYHHLYGLDCTVFRFFTVYGPRGRTDMMAYKVLDNIF
                     TGCEVPLYNNGNMHRDWTYVEDIVSGLVAAVDRPMGYEILNLGRGEPVLLVDFVKGIE
                     QLTGRQAQL"
     gene            1101..1406
                     /locus_tag="VLA60_00075"
     CDS             1101..1406
                     /locus_tag="VLA60_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HSF07773.1"
                     /translation="MLYPIVCGIVKDPETLETTDLGTGKDFEAEVVVYNCDCHTYQQV
                     VNLFCQVIPGMTPKKAFELAWQIDHHGSAIVFQGDIKNADVIGKQLAAGGLRVEVRY"
     gene            complement(1446..1928)
                     /locus_tag="VLA60_00080"
     CDS             complement(1446..1928)
                     /locus_tag="VLA60_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07774.1"
                     /translation="MERRVTSHTEEEEMNQRRRLLTLARKGDQKAVESLFELYQVRVF
                     SGDALKKKKLPSFPLPMPTGSSGKGPGKGVKKMGKISRASPADVVPVPLPLNSGQESK
                     MKSGLPTKKIGKVKGAKPSAGKSVQTAPKVGKSKKSAPVAKAKKTSTLSARPARVKKK
                     "
     gene            2474..3295
                     /locus_tag="VLA60_00085"
     CDS             2474..3295
                     /locus_tag="VLA60_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSF07775.1"
                     /translation="MTQRTTPYDIEQIGYTLVRFNSTGIFETHKDHMVDPYADTRPPK
                     GPQVEGIQFAPQEANKILPAMVLLHDRYGLTSHIQELAKGLSCQGYVVLVPNLYVRQG
                     GMVTANAEVANALMERVNEQQALQDINASFEFLNANLTEDSLLERTTRNAHAVIGFGM
                     GGTLAIKTAAHRRRLQAAVAICGTLPTDLQLAQRLYCPLLLQTPGQSVLNSEEERDQF
                     CQMAKEAGKKIEVRSYPEASDEFWQSSTPSYRASDMEEALQTSIDFINAIINKTI"
     gene            3382..4545
                     /locus_tag="VLA60_00090"
     CDS             3382..4545
                     /locus_tag="VLA60_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07776.1"
                     /translation="MAWRGNRNIWLIVWVIGAHIFIFCSLSPAFANQESTGLPWSFLK
                     EHRTEILQLTSDDEALAFIKSTTTIEGRDSRIYMNSQSVPTQLNLPAEIIEAITSYLK
                     SLAAANHAGLFREILVKPTTDIPPLSDIIPGEAQLQWIMTHSTLQGLKPIIDGYRQVT
                     AWSHMTTSHPVPPEGEFAKFASYYDQTYQDWDESPQSWTSLFTQHGQKGIEDRLLEYW
                     QTSNHTADPHQSFPPIHDAYTQHYIQTRLLPMFRMILLTKTSELEARAYGTAWEAWQK
                     IQQWQQQEQANSASTRLCGTWRWVVHNHQNHGDRKMTLTFAPPGQPSPSQIAPTAIEI
                     HGDTVYLKWAFPQGVQEDSLLLSNNDSHLEGTFKNSLGPYGSISGKRLSTCQP"
     gene            4629..7619
                     /locus_tag="VLA60_00095"
     CDS             4629..7619
                     /locus_tag="VLA60_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HSF07777.1"
                     /translation="MIAHDSLESEIRRIGQTLAKNARHHTPGFWDHRWWTSLLLDWGT
                     RDEQFKVQLFRFIDVLPSLQTDAQFVRILKEYFQDLPSLPGPLNWALSRFSNNSLTAQ
                     VGTRILRRQFLKMAYTFMAGKTVSHAVPTLTQLWHAGSACSLDLLGESTVSEEEADHY
                     HTRCLQTLTQLHEVIPQWNHQPLLETDHLGSLPRINLSIKLSALYSQLDPIDPEGSYA
                     GVAPRLRSILDLAQALPASLTFDMEQAELEPIILRVFMRVFSEPAYQHFPHASIAIQA
                     YLKHSNDALDTLLAWGKQRNTPFGIRLVKGAYWDSEVIRYQQQGWPIPVYLKKPDTDA
                     NYENLARRILEHRAFIRPAFGSHNIRTLAVVRAMGDSLNLSPGTFEYQMLYGMAEPLR
                     DAVVEQGFRLRVYAPIGELLPGMAYLVRRLLENTSNESFIRRQYETAESLDHLLLSPS
                     NGNGNGEEAMSLEIQSEATFLEGDNPARFANEPHSDFSQQHIQRDFSLGLAHIAPLLG
                     QTHSYPLPTPMPWLGPELISTNPSFPDQIIAKFPTLSPKQIDPIVQRAHDHISTWGKV
                     PSHTRARILFHAAGLMRQRKPELAAWEILETGKPWREADADVAEAIDFLEFYGREMIR
                     LGTPERLGTEPGEHNQRIFQPRGLAVVISPWNFSLAIPTGLVSAALVAGNVVLFKPSE
                     RSPMMGYHLFTLLMEAGLPEGVLQFLPGGPDVGQALVKHPKIHLIAFTGSQTVGLNII
                     QEASHVLAGQRHIKHVIAEMGGKNAIIVDETADLDEAVVGALASATGYQGQKCSACSR
                     ILILKDVYPQFLERLKQAASSIPIGPPENPGNRMGPLIDRRALERVRHFVQLGKTEGT
                     CILDRQLEGPGYFQGPVILANLPPSHPVVQEEIFGPVMVVLKVSTISEALELANDTPY
                     ALTGGIYSRSPANIQLARDTFDVGNLYINRPITGSLVSRQPFGGHRLSGIGRKAGGSG
                     YLEQFMVEKVITENTLRRGFAPTQ"
     gene            complement(7512..8396)
                     /locus_tag="VLA60_00100"
     CDS             complement(7512..8396)
                     /locus_tag="VLA60_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07778.1"
                     /translation="MSTVGALVFLGLSLLSFPGTVHSADSTKIVIDKSHEYFPDQVGN
                     EWVYQGTMIEGGMVQIKNEDTRFTNVSKVIGTEVLEGVKVTVFNDTNPGNQGPSDSYY
                     FRDVAGIRYYGSKPGTTLERQLVPYQIVRFPLEVPSSFEQFHRTGLDFGMDVDRDGEK
                     ETVDIVATVSVQGHDTVIVPAGIYQEAIRLEAKMIMTVHLSESGGSIQGTDTMTAWFA
                     RGVGLLKYIERQTIPSVRSQNERMVEVIEELEKVTLASDVALLSRSEATAKRVFRNHL
                     FHHELLKIPASSGLPADS"
     gene            8705..9466
                     /locus_tag="VLA60_00105"
     CDS             8705..9466
                     /locus_tag="VLA60_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF07779.1"
                     /translation="MNEPFCQTPMGKVNTRPNVMVFGGSGIIGGAIATRFGCQGWRVG
                     LHYHQHQTVAKKTAAVITKAGGESHLYQADVTNSSQIQAILRKFIQSYHSLNVIVWAV
                     GMSSSKLLVKTSPEDWTRILHTNLTGAYTVLKAIAPIFEQQNYGSVILVGSLSGEQGV
                     TGQAAYAASKAGLVGLMHTVAKEWGAFNTRVNMVFPGWHLSPISAPGWDAAMEPRNHT
                     LHRTPSLQYVATSIYHMALSPDTSGQIWNLDSRIW"
     gene            9439..10242
                     /gene="bioD"
                     /locus_tag="VLA60_00110"
     CDS             9439..10242
                     /gene="bioD"
                     /locus_tag="VLA60_00110"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HSF07780.1"
                     /translation="MESGQPDLVIQFILQPCRDMSTHPDHLAIFITATDTGVGKTTIT
                     AALVLGLKKQGYQVGVMKPVETGIDPQQTETSDTVRLQSLLSPPPPFAAICLYAFPQP
                     VAPLTCARATGTTIDLARIATAFHRLRQQHPVCLVEGAGGLLTPLSPTQTIRDLIATL
                     NLPALIVGRTSLGSVNHMLLTLEALNRAGIKSRGIVLNDPISTTQTESFIQQRTSTIR
                     LIQEWSEVPVFGPLEFQKTIQRDWRTGVENLADHPEIQRLARHIIETEP"
     gene            complement(10223..10876)
                     /gene="thiE"
                     /locus_tag="VLA60_00115"
     CDS             complement(10223..10876)
                     /gene="thiE"
                     /locus_tag="VLA60_00115"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSF07781.1"
                     /translation="MTADHTSQIFPPDIFSRFYLIVDDHWASRCSLLEVLQQASEAGV
                     MLVQYRNKTGSMKQAYEAGLALRKVAAERGMTFIVNDRCDLALALEADGVHLGQGDLP
                     LLLARKVVGQKMLIGVSTHNPEQVRLATAEGADYLGFGPIFSTRTKTNHEPVVGIQGL
                     AGVRSLTTLPIIAIGGIVPGSVPALQGAGANGVAVASAILDAVDPPRILAEFMAQFQ"
     gene            complement(10873..12222)
                     /gene="accC"
                     /locus_tag="VLA60_00120"
     CDS             complement(10873..12222)
                     /gene="accC"
                     /locus_tag="VLA60_00120"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSF07782.1"
                     /translation="MFKKILIANRGEIALRVNRACRELGIRTVAIHSDVDAQSLHVRY
                     ADEHVCVGPAEGGLSYRNIPNVLSAAEITGVDAIHPGYGFLAENAHFAEVCESIGITF
                     IGPTSEHIALLSDKSKARAHMKKRGIPVLPGSEGEIDDPKTCVAIAKKLGFPVIVKAS
                     AGGGGRGMRVVWHEEELTQAIESAQLEAQTAFGHGGVYLERFFEDPRHIEFQIMADNH
                     GHVVHFHERDCSIQRRYQKVLEESPSPALDDTLRRKMGQVAVEAIKSVKYRNAGTVEF
                     LMDQSGKFYFMEVNTRIQVEHPVTEMVTGVDLIKEQIRIAAGEELSYRQKDIQLTGHA
                     IECRINAECPDRFTPSPGTITKFCPPGGPGIRVDTAMDTTGIVHPYYDSMIAKLIAFG
                     RDRNEAIARTRRALDEFVIDGIKTSIPLHRRILDHPDFQKGPVSTRFLDRLVALQSP"
     gene            complement(12318..12782)
                     /gene="accB"
                     /locus_tag="VLA60_00125"
     CDS             complement(12318..12782)
                     /gene="accB"
                     /locus_tag="VLA60_00125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HSF07783.1"
                     /translation="MADSSRREIEDLVEVLNRYHLTELEFEKKGVRIRIRRDHVPASS
                     HQGDVSKPVEAGSSQERPVPLLPSEPAHFLTVTSPIVGTFYRSPSPDTDPYVEEGDIV
                     KKGQVLCIVEAMKLMNEIEAETDGKVVKILVESTTPVEFGQPLYLIDPLSGS"
     gene            complement(12825..13385)
                     /gene="efp"
                     /locus_tag="VLA60_00130"
     CDS             complement(12825..13385)
                     /gene="efp"
                     /locus_tag="VLA60_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HSF07784.1"
                     /translation="MITTADFRNGSRLELDGQPYYIVEFQHVKPGKGGAFVRTKLKGY
                     KTGNVIDRTFRSGEKFDEPNLEECDMQFLYNAGEEYAFMDISSFEQFTYLKNQLGENV
                     DLLKENTVVKILLYHDEPIAVELPVFMELKVVETDPGIRGDTASGGTKLAVVETGASV
                     KVPLYLESGEVIKIDTRTRTYVERAR"
     gene            complement(13425..13877)
                     /gene="aroQ"
                     /locus_tag="VLA60_00135"
     CDS             complement(13425..13877)
                     /gene="aroQ"
                     /locus_tag="VLA60_00135"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HSF07785.1"
                     /translation="MNILVLHGPNLNLLGTREPNLYGTITLEEVNASLKKLGMDLGVS
                     VESRQSNLEGELVTWIQEANAQFEGLVFNPAAYTHTSIALRDAVVGVGIPMVEVHLSN
                     IHRRESFRRRSYLAPVSLGQISGFGPQSYLLGLRALVDALQRTIPAHR"
     gene            complement(14026..15513)
                     /locus_tag="VLA60_00140"
     CDS             complement(14026..15513)
                     /locus_tag="VLA60_00140"
                     /inference="COORDINATES: protein motif:HMM:NF022525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HSF07786.1"
                     /translation="MNRSVSTHRLSKSRFLAGLQCAKRLYLEIHSPELATPPTPDRRA
                     IMDMGTDVGVVARQYFPHGILVEHTHRQIPAALLRTSELVKDVGIPSLFEGAFVWQGI
                     LVRVDILERIDATRWRLIEVKATSKVKATHLDDLAIQAAVLEGAGIEVSGCFLMHLNT
                     QYSFLGGDLNLEEMFVLENKTEEVESRRPLIQAQLEDMWMTLEQATPPPIVPDSHCHQ
                     PYECPFWNHCTKGKSQRWVFHLPGSSKLQTLLLEEGIETIDEIPDHIQLSATQQRVKD
                     NVEWISPLLRPRLQSVRFPVHHLDFETFMPAIPAFPHTRPYRPIPFQWSNHIEYPDGT
                     VVHHHYLCTDGRDPREEVALSLLESVGDAGTICVYSEYERFLLFALGDVLPQLKPVLS
                     KVVRRLWDLLSVIQEHYYHPDFHGSYSIKTVLPALVPALAYDDLTIQNGAVAAVMYQK
                     MVFHETDLMERAHIAQALHEYCGRDTWAMVELRRVLLDRAGGRLT"
     gene            complement(16121..17293)
                     /gene="hemW"
                     /locus_tag="VLA60_00145"
     CDS             complement(16121..17293)
                     /gene="hemW"
                     /locus_tag="VLA60_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HSF07787.1"
                     /translation="MEQKREHSGEHDGFTADLGIYLHVPFCKKRCHFCAFYLVLHDQQ
                     RVKQFLQAIEREIALYASQPGMLERTVSTVYIGGGTPTVLRPGQLATIFSSIRSGFSL
                     SKQCEITVEATPESLTDQYADFLRQAGVTRVSIGVQSFDPQERTTLGLSGSMAEAISG
                     VRIAKQAGFANINLDLIYGIPSQTVESWEMTLTQALELDPSHLSLYALSLEKGTRFYT
                     EFRRGLFEVMDPDHEACFLQQAETHLEPQQLSRYELSNWAKPGYACQHNLRYWRGLEY
                     LGLGPSAQSYVLESRYGNVSSIEQYSKRLRAGQLPIANRELLSRAQQDKERIVFGLRL
                     LEGVPIEWIQQASQNEVWATSFEAMVKEDYLFQTDTGVQLTQKGRQFADTVGMRLL"
     gene            complement(17444..18160)
                     /locus_tag="VLA60_00150"
     CDS             complement(17444..18160)
                     /locus_tag="VLA60_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSF07788.1"
                     /translation="MTPRRFRAKVLRIRHLTQDVRELILELVDTPTFEFLPGQSIAVT
                     IPDGASGLSYLRYYSVASLPSSSHQLILLFNAGEKGKGATFALEHSVGEELDCSGPFG
                     SFHLHEDPERDLLFVGTGTGIAPLWSMLSSLFEQSCSQPVTLLWGLRSESDIYYLDEL
                     QKWADCYKNFSFILTLSQPSHHWRGKRGRVTHLLQEFPHVDHLAVYVCGNRKMVAEVT
                     SLLQCKGVCPVYRERHHDGK"
     gene            complement(18419..>20402)
                     /locus_tag="VLA60_00155"
     CDS             complement(18419..>20402)
                     /locus_tag="VLA60_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HSF07789.1"
                     /translation="DEYFWLRDRENPEVLAYLQAENEYAATLRSHTRDLEQALFEEIC
                     GRIQPTDLSVPFRLGDFWYYIRYEEGKEYALYCRKEKSLTNPESIMVDGNELARGHDY
                     FSLGNWAISPGQDILAYAIDTQGRRIFTIGFKNLTTGETIDECIPLVTGNMEWGNDNR
                     TLIYSRQDPETLRSNQIFRHRLGTNPELDTLVYEETDETFSVSIEKTKSKRYLMIGSH
                     QSITSEYRYVNADHPESDFRIFQARKRGHEYDVDHLGDHFFIRSNDEAKNFRLMRTET
                     NKTNLAQWEEVIPHRPEVFLEGFELFENFLVLEERRQGLIHLRMIRTTDGSEHELDFG
                     EPAYLATLGDNWEADTSYLRYGYTSMTTPMTIYDYHMETREKILLKQEPVLGNFHISH
                     YQTERLFAPASDGISIPISLVYRKGFAPNGTHSLLLYGYGSYGASMDASFSSPRLSLL
                     DRGFVYALAHIRGGEELGRHWYENGKLLYKKNTFTDFIACAEFLIQQGYAAPQKLFAL
                     GGSAGGLLMGAIMNMRPDLFYGVVAQVPFVDVVTTMLDPNIPLTTGEYDEWGDPNQQQ
                     FYEYMLSYSPYDNLQPQSYPHLLVTSGLHDSQVQFWEPTKWVAKLRALKTDNQRLLLR
                     TNMDAGHSGASGRFKRYEETAMIYAFLLDLAGSVDL"
BASE COUNT         5451 a         5437 c         4521 g         4993 t
ORIGIN      
        1 gagctgtgcc tggcgccctg ttaattgttc aatgcctttc acaaagtcaa ccagaagaac
       61 cggttcgcct cgtccaagat tcaaaatttc ataccccatc ggacgatcaa ccgccgcgac
      121 caggccactg acaatatctt cgacatacgt ccaatctcga tgcatgtttc cattgttata
      181 gagggggact tcacagccgg tgaatatgtt atctaacact ttataggcca tcatatcggt
      241 acggccacgc ggtccataga cggtaaaaaa tcgaaagacg gtgcaatcca aaccatacaa
      301 gtggtgatag gtatacccta gcaattcact tgctcgctta ctcgccgcat atggggcgag
      361 cggtttatca cacgaatcct ccggaacaaa ggggatcgtc ttggtattgc catacaccga
      421 agatgttgat gcaaacacaa acgttggaga aggtgaatgt ttgccctgcg aaccaatccg
      481 ccccacgcac gcatccaata aggccatcgt tcccatcaca ttgacatcgt aatacagtgc
      541 aggatcatca atggacaccc gaactcccgc catcgccgct aaatggacga cagcgtcaaa
      601 acgatgatca gaaaaaagat gtgcaaccaa ctctcgatca cgaatatctc ctttgagaaa
      661 ctgaaacgtg ccaggccatt ttccggtttt ggcatgggcg gtcacctctc caaggttttt
      721 ctctttacgg acaggatcat agtaatcatt gagattatca agaccaatta cttgatctcc
      781 acgtctgacc aatgcttcca ctgcatggct tccaataaat cccgcagcac cggttaccaa
      841 tatcgtttta cccacggata ctcctttatg ttccttagag ggttcatatt tggcacatct
      901 tcttgctgac ttttaaattg atacccatct gcattatttt ttttcggtaa ctaccgtgtc
      961 atgcttgata agcaaggaca atcataggtc gagtatccct aatcatggtt cacttaattt
     1021 tcctgaatca gactttcgtc aaaatgacga cttactctat attcacttga gggacctcta
     1081 ctccattgct acaatgcgga atgctctacc ctattgtgtg tgggatcgtc aaagatcccg
     1141 aaaccctaga aactaccgac ctaggtaccg gaaaagattt cgaagccgaa gtcgttgtct
     1201 acaattgtga ttgtcatacc taccaacaag tcgtcaatct tttttgtcaa gtgattcctg
     1261 ggatgacccc gaaaaaagcc tttgaattag cctggcagat tgatcaccac ggaagtgcaa
     1321 tagtctttca aggtgacata aaaaacgctg atgtaattgg aaagcagctg gcagcaggtg
     1381 gcttgagagt tgaagtccgc tactgaatct gacaccacac gacagaatcg tcctctcacc
     1441 cgaagttatt ttttcttcac gcgggccggt ctcgcagaca aggtagatgt ttttttggct
     1501 ttggcgactg gagcagattt tttacttttt ccaactttgg gagcagtttg tacagacttc
     1561 ccggcagagg gctttgcccc tttgaccttt ccaattttct tggtgggcaa tcccgacttc
     1621 attttggact cttgccctga attgagaggt aaggggacag gcaccacgtc tgcgggagag
     1681 gccctagaga ttttacccat tttcttcacg cccttccccg ggcctttccc cgaagatcct
     1741 gttggcatgg gcaatgggaa cgatgggagt ttcttttttt tcagggcgtc acctgaaaac
     1801 acacgaactt gatacaattc gaacaacgat tcaaccgcct tttgatcccc tttccgtgca
     1861 agcgtgagaa ggcgacggcg ttgattcatt tcttcttctt ccgtatggga agtgactcgc
     1921 cgctccatgc tcaactcctt tccttatccc gaaatcaaac tttctgacat tccactgcag
     1981 accgcatctt acactaaacc ccatgacatt ggcaatattc cctaatgaac atttctagtc
     2041 tttagaacgt aaaattttta taagaaaaaa ttcacatgac ttttgctcaa actgaagaat
     2101 gggagaatta ccaacaagag gccaggctac aaaattaagg caagacgcaa tagcctttga
     2161 ttttttcgtg aatgaccttg gtggcctttc tcccgtcgca aaagaggtgc ccatcattct
     2221 gagttggtcc tgatgtaagg aaaaacgtta gacgtgattg tcccttttca ccagagaata
     2281 tttgggattg aatcgttctc ctaacgacga acccactgag aaccttcgca ttaccctttc
     2341 taccctgaac aacagctgct tgattgacag gccaaagaaa ccaccgatat agtgttttga
     2401 ctttccccat tgaattttca aagaaatttc cattttgtgc cgcttcgttc attcttatcc
     2461 aaaggctatt ctgatgaccc aacgcaccac cccctatgac attgaacaaa ttggatatac
     2521 ccttgtgcga tttaatagta caggaatatt tgaaacgcac aaggaccaca tggtagatcc
     2581 ctatgccgat acacgacccc ctaaaggccc gcaggtggag ggcattcaat tcgccccaca
     2641 agaggccaac aaaatcttac cggctatggt cctcctgcat gatcgctatg gactcacgtc
     2701 tcacattcag gaattagcga aaggtctctc ctgccaaggg tacgtggtct tagttcccaa
     2761 tttgtatgtg agacagggcg ggatggtgac ggccaatgct gaagtggcca atgccttaat
     2821 ggagcgagtc aatgaacagc aagccctgca ggatatcaac gccagctttg aatttcttaa
     2881 cgccaacctc acagaagatt ccttattaga aagaaccacc cgcaacgcac atgcagtgat
     2941 cgggtttggc atggggggaa ccctggcaat aaaaactgcc gcgcatcggc gtcgccttca
     3001 ggccgcagtg gcaatctgtg gcactctgcc aaccgatctt caattggctc aacgcttgta
     3061 ttgccctctc ttactccaga caccaggaca aagcgttctc aattcggaag aggaacgtga
     3121 ccagttttgc caaatggcca aagaagctgg gaaaaaaata gaagttcgat cctatcctga
     3181 agcctctgat gaattctggc aaagtagcac tcctagctat cgggcatcag atatggaaga
     3241 agccctgcag acctcaatag acttcatcaa tgcgatcatc aataaaacca tataagcccc
     3301 aatgtgattt tcgaaaacca tggccgactc gtttcatcaa ttcatccctt ccgagcacta
     3361 acgcttacct tgagaaatct catggcttgg cgtggcaatc gaaatatatg gttgattgtc
     3421 tgggttattg gcgcccacat ttttattttc tgttctcttt cccccgcctt tgccaatcag
     3481 gaatcaaccg gactcccctg gtccttcctt aaagaacacc gaacagaaat tctccagctc
     3541 acctccgatg acgaagccct agccttcatc aaatccacaa ccactataga gggcagagac
     3601 tctcgtattt acatgaattc acaatcggta cctacgcagt tgaacctgcc agcagagatc
     3661 atcgaggcca tcaccagcta tttaaaatct ctggctgccg ccaaccacgc aggattattt
     3721 cgggagatcc tggtaaagcc aacaacagac atcccgccac tttccgacat catccccggc
     3781 gaagcacaac tgcaatggat catgacacac tccacccttc aaggcttgaa acccataatt
     3841 gatggctata gacaagtaac agcctggtct cacatgacga cttcccatcc agtccctcct
     3901 gagggagaat ttgccaaatt tgcttcatat tacgaccaaa cttatcagga ttgggatgaa
     3961 agcccacaat cctggacaag cctttttaca cagcacggcc aaaagggaat cgaggatcga
     4021 ttactcgagt attggcaaac atcgaatcac acggccgatc cgcatcagtc cttcccaccc
     4081 attcatgatg cctataccca gcattacatc caaacccggc tcttaccgat gttcagaatg
     4141 atccttctca ccaaaaccag cgagttggag gccagggcgt atgggacagc atgggaggcc
     4201 tggcaaaaaa tccagcagtg gcaacaacag gaacaagcca attctgccag cactcggctg
     4261 tgcggaacct ggcgatgggt ggtacataac caccaaaacc atggggatcg taaaatgacc
     4321 ctcacctttg cgccccctgg acaaccctca ccctctcaaa ttgccccaac cgccattgag
     4381 attcacgggg atacggtcta tctcaaatgg gcctttcccc aaggagttca ggaagatagc
     4441 ttgctcctca gcaacaatga ctcccatctt gaaggcactt tcaaaaactc cctcggccct
     4501 tatggcagca tttcgggtaa acgtttgtcg acttgccaac cctagtcttt cttcagagtt
     4561 cgtttctcct ttatctctct tccgcattct ccgaaaaggt gtaaaataac cttaagaggg
     4621 aaaacaggat gattgcccat gattcattag aatctgagat tcggcgtatt ggacagacat
     4681 tagcgaaaaa tgctcgtcac catacgccag gattctggga tcaccgctgg tggacgtcac
     4741 ttctcttaga ttggggaacc cgtgatgaac agttcaaggt acaactgttt cgctttattg
     4801 acgtccttcc ctcccttcag acggacgcac aatttgtgcg tatactgaag gaatactttc
     4861 aagacctacc gtctctccct ggtcccctga attgggcact tagtcggttt tcaaacaatt
     4921 cccttacggc ccaggtgggc acacggatat tgcggcgcca gttcctgaaa atggcctaca
     4981 cgtttatggc agggaaaacc gtcagccacg ctgtccccac gctcacacaa ctttggcatg
     5041 caggaagtgc atgttctctt gatttgcttg gagaatccac ggtcagtgaa gaggaggctg
     5101 atcattatca cacgcgatgc cttcagacgt tgacccaatt acatgaagtc atcccacaat
     5161 ggaatcatca acctctatta gaaaccgatc atctcggttc tctccccagg atcaatctgt
     5221 ccatcaaact ctccgcatta tattcgcaat tagacccgat tgatcctgag ggcagttacg
     5281 caggcgttgc cccccgccta cgatcgatcc tggaccttgc ccaggctctg ccggcctctc
     5341 tcacgtttga catggagcag gccgaattgg agccgatcat tcttagggtc tttatgcgtg
     5401 tattttctga acctgcctac caacactttc cgcatgcaag cattgccata caagcctatc
     5461 tcaaacacag taacgacgcg ctggatactc ttctagcatg gggaaaacaa cgaaacactc
     5521 ccttcggtat tcgcctcgtc aagggcgcct actgggactc tgaagttatt cgctatcaac
     5581 aacaaggctg gcccatcccg gtctatctta aaaaaccgga caccgatgcg aactacgaaa
     5641 acctcgctcg ccgaattctg gaacatcgag ccttcattcg cccggctttt ggctcgcaca
     5701 atattcgaac cttagccgtg gtccgggcaa tgggagactc gctcaatctc tccccgggaa
     5761 cgtttgaata tcagatgctg tatggcatgg ccgagcctct acgtgatgcc gtggtggaac
     5821 aagggtttcg gctcagggtc tatgccccca ttggggaact ccttcctggt atggcctatc
     5881 tcgtgcgtcg attattggaa aatacctcga atgaaagttt cattcgacgc caatatgaaa
     5941 ctgccgagtc acttgatcat ctgctattat caccttcgaa cgggaacggg aatggagagg
     6001 aggcgatgtc tctggaaatt cagtcggaag ccacgtttct cgagggagac aatcctgcca
     6061 ggttcgccaa cgaaccgcat tcggattttt ctcaacagca catacaacga gatttctctc
     6121 tcggcctcgc acacatcgcc cctctccttg gacaaaccca ttcctatccg cttccgaccc
     6181 ccatgccctg gctagggcca gaactgatct cgactaatcc cagttttcct gaccaaatca
     6241 ttgccaaatt tccaactctg tctcctaaac aaattgatcc cattgtccag cgcgcccacg
     6301 accatataag cacatgggga aaagtccctt cgcacacccg tgcgcggatt ttatttcatg
     6361 cagccgggtt gatgcgacaa cgcaagcctg aattagccgc ttgggaaata ttggaaaccg
     6421 gaaagccttg gcgggaagcc gatgcagacg tcgctgaagc catcgatttc ctggagtttt
     6481 atggaaggga aatgattcgt ctgggcactc cggagcggct aggaacagaa ccaggggaac
     6541 acaatcaacg tattttccaa ccacgagggt tggccgtggt tatttcgccg tggaattttt
     6601 cgttagccat ccccaccggt cttgtttcgg cggccttagt ggctggaaat gttgtcctat
     6661 ttaaaccatc cgagcgttcc ccgatgatgg ggtatcacct cttcacgttg ctcatggaag
     6721 cagggttacc tgaaggcgtt ttgcagtttc tgcccggtgg acctgacgtg ggacaggccc
     6781 ttgtcaaaca ccccaagata cacctcatcg cctttacagg atcacaaacc gtcggattga
     6841 atatcattca agaggccagt cacgtgttag ctgggcaacg acatatcaaa catgtcattg
     6901 ccgaaatggg cggaaaaaat gccatcattg ttgatgaaac ggctgatttg gatgaagccg
     6961 tggttggagc cttagccagt gcaacaggct accaaggaca aaaatgctcc gcctgttcca
     7021 gaattcttat cctcaaggac gtgtaccccc aatttcttga acggctcaaa caggcggctt
     7081 caagcattcc gattggtcca ccggaaaacc ctggaaaccg tatgggaccg cttattgatc
     7141 gacgggcact ggaacgagtt cggcactttg tgcaacttgg gaaaacagaa ggcacctgca
     7201 ttcttgatcg ccagcttgag ggacctgggt attttcaagg gccggtgata ctggcaaatc
     7261 tgccaccttc ccacccggtc gttcaggaag aaatatttgg cccggtcatg gttgttttga
     7321 aggtctccac gatttcggaa gcgctggaat tagccaatga taccccctat gccttgactg
     7381 gagggatata ctcacgcagt cctgccaata ttcaactggc tcgtgacaca tttgatgtgg
     7441 ggaatttgta tatcaaccga ccgattaccg gatcattagt gagcaggcaa ccctttggag
     7501 ggcatcggtt atcaggaatc ggccggaagg ccggaggaag cgggtatctt gagcaattca
     7561 tggtggaaaa ggtgattacg gaaaacacgc ttcgccgtgg cttcgctccg actcaataaa
     7621 gcgacatcag atgccagggt caccttttct aattcctcaa tcacttcaac catccgttca
     7681 ttctggctgc gaacggaagg aatggtttgg cgttcaatat atttgagcaa tcccactcct
     7741 cgagcaaacc aggccgtcat cgtatccgtt ccttgaatcg accccccgct ctcagacaaa
     7801 tgcaccgtca tgatcatttt ggcttcaaga cgaatggctt cctgatatat gccagccggt
     7861 acgatcacgg tatcgtgccc ctgaacagac acggttgcca caatatccac cgtttctttt
     7921 tctccatcac gatccacatc catgccaaaa tccaacccgg ttcggtgaaa ttgctcaaaa
     7981 gagctgggta cctctaaggg aaatcgaacg atttgatagg gcaccagttg gcgctccaac
     8041 gtcgtgccgg gtttcgatcc ataataccgg atccctgcaa catcacgaaa ataataacta
     8101 tccgacggcc cttgattccc aggattggta tcgttaaaaa ccgtgacctt cacgccttct
     8161 aggacttcgg tccctataac ttttgaaaca ttcgtaaacc gggtatcctc attcttaatc
     8221 tggaccatgc ccccttcgat catcgtgccc tgatacaccc attcatttcc cacctgatca
     8281 ggaaaatact catgactttt atctatcaca attttcgtgg aatcagcgga atggaccgtg
     8341 cccggaaagg acaacaaaga aagaccaagg aaaaccaagg ctccaactgt ggacaaaatt
     8401 ccacacgatg taaaggaatt tttcataggc agaaagtgat gtgaacctac cataggatca
     8461 ttaaaaccgg caagacacca accaatatct acaaatccag gccgcattca acgtgaaaac
     8521 cccgcaattt gtcttgacag actgttcaag aattgacaat ttctcttccc tatgcagtac
     8581 tagaaacgaa ggagaccgaa tgggagacaa tgcaggacga caccaggtca gtcaaatttc
     8641 acccctctca tcgcgcttct ttacctttgc atgatgtgat gggagtgaca ggtgggggcc
     8701 aaaaatgaac gaaccttttt gccaaacacc tatgggcaag gtgaataccc gtccgaacgt
     8761 catggtgttt ggaggatccg gaatcatcgg tggagccatt gcgacgcggt tcggatgcca
     8821 gggttggaga gtaggccttc attaccatca acatcaaact gttgcaaaaa aaaccgccgc
     8881 cgtgatcacc aaggccggag gagaaagtca tctgtatcaa gccgacgtca ccaacagctc
     8941 acaaattcag gctattctgc ggaagttcat acagtcctat cattctctga atgtcatagt
     9001 ctgggccgtg gggatgagct cttcgaagct cctagtcaaa acctcccctg aggactggac
     9061 ccggattctt cacaccaacc tgaccggtgc ctataccgta cttaaagcca tagcccccat
     9121 ttttgaacaa caaaattatg ggtccgtcat tttggtaggc tctctttctg gtgaacaagg
     9181 agtaacagga caagccgcct atgcggcgtc taaggcagga ctggttggat tgatgcacac
     9241 agtcgcaaag gagtgggggg ccttcaatac tcgagtgaat atggtattcc caggctggca
     9301 cctctcacca atatcagcac cgggatggga tgcggccatg gagccccgca accatacgct
     9361 ccacagaact ccttctctac aatatgtggc cacgtctatc tatcacatgg ccttatcgcc
     9421 tgatacgtct ggacaaatat ggaatctgga cagccggatt tggtgatcca attcattctt
     9481 cagccttgcc gtgacatgag cactcaccct gaccatctcg ccatattcat aaccgcgacc
     9541 gacaccgggg tcggtaaaac aacgatcacg gcggctctgg ttctggggtt aaaaaaacaa
     9601 gggtatcaag tgggtgtcat gaagccggtc gagaccggta tcgatcctca acagacagaa
     9661 acttctgaca cagtccgtct tcaaagtcta ctctcccctc ctcccccctt cgcagcaatc
     9721 tgtctctatg cctttcctca gcccgttgca ccgttaactt gcgcgcgcgc gactggaaca
     9781 accatcgatc tcgcccgaat tgccaccgcc tttcatcgtc tcaggcaaca gcaccccgtg
     9841 tgtctcgttg aaggggctgg aggtttactt actccactct caccaacaca gaccatacgc
     9901 gacctgattg cgaccctgaa tcttcccgct ctcatagtcg gccggacaag cctgggcagc
     9961 gtgaaccata tgctcctgac tttggaagca ttgaaccgtg ccgggattaa gtcccgtgga
    10021 attgtcctga atgatcccat ttccacaact caaaccgaga gtttcattca gcagcgaacc
    10081 tccacgattc gcctgattca agaatggtcg gaagtgcctg tatttggccc cttggagttt
    10141 caaaaaacca tccagaggga ttggcgaacg ggagtggaaa acctagccga ccatcctgaa
    10201 attcaacgat tggccaggca tattattgaa actgagccat aaactccgcc aggatccttg
    10261 gcggatcaac cgcatcgagt atggccgaag ccaccgccac gccattggcc cctgcgcctt
    10321 ggagtgccgg aacggaaccg ggaacaatcc caccaatggc aatgatggga agagtggtca
    10381 ggcttcgaac accagccagt ccttgaatac ccacgaccgg ctcatgattc gttttggttc
    10441 ttgttgaaaa aatagggcca aaccctaaat aatccgcacc ttccgccgtg gccaatcgca
    10501 cctgttcagg attgtgtgtc gaaaccccaa tcaacatttt ctgtccgacg actttccgcg
    10561 caagaagcag gggaaggtcg ccctgaccaa gatgcacacc atcggcctcg agagctaacg
    10621 ccaaatcaca tcgatcattc acaataaacg tcatgcccct ctctgccgcc acctttcgaa
    10681 gggcaaggcc cgcttcatag gcctgtttca tcgatccggt tttattacga tattgcacca
    10741 gcatgacccc cgcttcacta gcctgttgga ggacctccag caacgaacat cgcgaggccc
    10801 agtggtcatc cacaatgaga tagaatcgtg agaaaatatc aggaggaaaa atttgcgaag
    10861 tatggtcggc ggtcatgggg actgtaaagc aacaagccgg tccaaaaatc gggtcgacac
    10921 tggccccttc tggaaatcag gatgatcaag aatccgtcga tggagaggaa tgctggtttt
    10981 aataccatca attacaaatt catccaacgc ccgtcgggtt cgagcgatag cttcatttcg
    11041 atctcgccca aaagcaataa gcttcgcaat catcgagtcg taatagggat ggacaattcc
    11101 ggttgtatcc atggccgtat cgacgcgtat tcctggtccg ccagggggac aaaattttgt
    11161 gatggtaccg gggctagggg taaatcggtc cggacattcg gcattgattc ggcattcaat
    11221 ggcatgtccg gtcagttgaa tatctttttg tcggtaggac agttcctccc cggcggcaat
    11281 acgaatttgc tccttaatca ggtcaactcc cgtcaccatt tcggtgaccg gatgctcaac
    11341 ttgaattcgg gtattgactt ccatgaaata aaactttcca ctttgatcca tgagaaactc
    11401 aacagttccg gcgtttcgat acttcacgga cttgatagcc tcaaccgcaa cctgccccat
    11461 ttttcttcgt aacgtgtcgt ccaacgctgg agagggggat tcctccaaca ccttttgata
    11521 tcgccgctgt atggaacaat cccgttcatg aaaatgcaca acatgcccat ggttatccgc
    11581 cataatctga aattcaatgt gtcgtggatc ttcaaaaaaa cgctcaagat acacaccccc
    11641 atggccaaac gctgtttgcg cttctaattg agcggactca atcgcctggg tcaactcttc
    11701 ttcatgccaa accacccgca tgcctcgccc tccgccaccg gctgaggctt tcacaatgac
    11761 cggaaaccca agctttttgg caatagcgac acaggttttg ggatcgtcaa tttcaccttc
    11821 gctcccaggc aacacgggga tcccccgctt cttcatgtgg gcccgggctt ttgatttatc
    11881 gctgagtaag gcaatatgct cagaggtggg cccaataaag gtaatcccaa tcgattcgca
    11941 gacttccgca aaatgcgcat tttcagctaa aaacccgtac cccgggtgaa tcgcatccac
    12001 tccggtaatt tcggcagcac tcaatacatt cgggatattt cgatagctca gtccgccttc
    12061 ggcaggtccg acgcacacat gctcgtctgc ataacggaca tggagtgatt gagcatcaac
    12121 gtcggaatga atcgcaacgg tgcgaatgcc caactcacgg cacgctcgat tcacccgaag
    12181 ggcaatttct ccacgattgg caattaatat tttcttaaac acgtcgtatc cttagataaa
    12241 aagactcgag ggaatcatcg taaccctcaa tcaagagcaa cagacttttc gcattcccct
    12301 tcagaaaaaa aaagagttca ggaccccgac aacggatcaa ttaaatagag gggttggccg
    12361 aactcaaccg gagtcgtact ttccacgaga atttttacga cttttccatc ggtttctgcc
    12421 tcaatttcat tcatcagttt catggcttcg acaatgcaga gcacctgccc ttttttgacg
    12481 atatcacctt cttcgacata gggatccgta tcaggagagg gagaacgata aaaggttccc
    12541 acaatgggcg aggtcacagt caagaaatgg gcgggttcgg agggaaggag agggaccggc
    12601 ctttcctggg aagaaccagc ctctacgggc tttgatacgt ccccctgatg ggaagaagcc
    12661 ggcacatggt cccgtctaat gcgtattcgc acaccttttt tttcgaattc caactcggtc
    12721 agatgatacc ggtttaatac ttcgacaagg tcttcgattt ctcttctgga tgaatcagcc
    12781 ataatctcct atttcgtgta tacctgcagg acaggaatcc cggattaccg agcccgttct
    12841 acataggtgc gcgtcctcgt atcaatttta ataacttctc cggattccag atagagaggc
    12901 acttttaccg aggccccggt ctcgacaacc gccaacttgg ttccaccgga tgccgtatct
    12961 cctcggatac cgggatcggt ttccacgacc ttcaactcca taaacacggg caactctacc
    13021 gcgatcggct catcatgata caacaaaatt ttgacgacgg tattttcctt gagcaaatcg
    13081 acgttttcgc ccaattggtt tttaagatag gtaaactgct caaaactact aatatccata
    13141 aacgcatatt cttcgccagc gttatagaga aactgcatgt cgcattcctc aagattgggt
    13201 tcgtcgaatt tttctccgga acgaaatgta cgatcaatga cattccccgt tttatagcct
    13261 ttcaattttg ttcgaacaaa ggctccgcct ttaccgggtt ttacatgttg aaattccaca
    13321 atataatagg gttgcccatc aagctccaag cgcgacccat ttcgaaaatc tgcagtcgta
    13381 atcacggcat caaactcctc atacaataaa cgtgaacaga aatgttaacg gtgtgctggg
    13441 atagtccgct gaagcgcatc aaccaacgct ctcagtccaa gcagataact ttgtggtcca
    13501 aatccagaga tttgcccgag cgaaaccggg gcaaggtagg accggcgacg aaaactttcc
    13561 cggcgatgga tattcgatag atgcacttcg accataggaa ttcccacacc cacaacagca
    13621 tctcgcaggg caatactggt atgggtatag gcagcaggat taaacaccaa cccctcaaat
    13681 tgtgcgttgg cttcctggat ccaggtgacc aattcccctt ccagattgga ttgccggctc
    13741 tcaaccgaga cccctaagtc cattcccaat tttttcagtg acgcattcac ctcctccaag
    13801 gtaatggttc catagaggtt cggctcacga gtcccaagta aattcagatt aggcccatgt
    13861 aataccagga tattcactag gtatcttcca taaataaatg gcgttggcga ggattcagga
    13921 tatctaagac acaggcatcg ttggactggt ggagtctggg aaagacggaa tcggtgaaat
    13981 tctagcagcg acagacgaat gggtcaacgg aatctgacgc acttcctaag tcaggcgacc
    14041 tcctgcacga tctagaagga cccgtcttag ctcaaccatt gcccatgtgt ccctgccaca
    14101 atattcatgt aacgcttggg caatatgagc acgctccatt aagtccgttt catgaaagac
    14161 cattttctgg tacatgacgg cagccaccgc cccattttga atcgttaaat catcgtaagc
    14221 taaggcaggc accaaagctg gcaagacggt tttaatggag taggatccgt gaaagtccgg
    14281 atgataataa tgttcctgga tgaccgaaag gagatcccat aatcggcgaa ccaccttcga
    14341 gaggacaggc ttcagctgag gcagcacatc acccagcgca aaaagcagaa atcgttcata
    14401 ctccgagtag acacaaatgg tcccggcatc tcctacgctt tccaacagac ttaaggccac
    14461 ttcttcgcga gggtcccggc catctgtgca cagataatga tgatggacaa ctgtgccatc
    14521 aggatattca atatggttag accactgaaa gggaatcgga cgatacggac gagtatgggg
    14581 aaaggccggg atcgccggca taaaagtctc aaaatccaaa tgatgaaccg ggaacctgac
    14641 tgattgcagc cgaggccgaa gcagaggtga aatccattcc acattatcct tcactcgttg
    14701 ctgagttgca gacaactgaa tatggtcggg aatctcatca atggtttcaa tcccctcctc
    14761 caggaggagg gtttgcaatt tcgagctgcc ggggagatga aacacccaac gctgagactt
    14821 ccctttcgta caatggttcc aaaagggaca ttcatacggc tgatggcaat ggctatcagg
    14881 gacaatggga ggtggcgtcg cctgttccaa tgtcatccac atatcttcaa gctgcgcctg
    14941 aatcaatggc cgacgcgatt ccacttcctc cgtcttattt tccaaaacaa acatttcttc
    15001 taaattcagg tctccgccaa gaaaagaata ctgagtattc agatgcatca aaaaacaccc
    15061 gctgacttca atgccggcac cctccaggac tgcagcttga atggccagat cgtctagatg
    15121 cgtggctttc accttcgaag tcgccttcac ttcaatcaat cgccagcgcg tggcatcaat
    15181 tcgctctaag atgtcaaccc gaaccaagat gccttgccag acaaaggccc cttcaaagag
    15241 ggacgggatg cctacatcct ttacgagctc actggtgcga aggagcgctg caggaatttg
    15301 acggtgtgtg tgttcaacca gaatgccatg aggaaaatat tgacgggcaa cgaccccaac
    15361 gtcggtaccc atatccatga tggcccggcg atccggcgta ggcggggtgg ctaattctgg
    15421 cgaatgaatt tccaaataca accgcttggc acattgcaaa cccgccagaa agcggctttt
    15481 ggataaacga tgcgttgaaa cagatcggtt caatttttct gattcggaaa cgagggggat
    15541 aggagaaatt gggttcatgg gattaatgac ggcacactag aaaaaagagg aaaattccaa
    15601 tctaaaacgt catttttaag aattttttca cccatccagg attcgaggac ttggatgaca
    15661 gcatactgct caattttttc ctcccatgca acatgcccgg ttattcaacc tccccgatac
    15721 attgcacagg aaaaattcga acagattacc tttatcccca tatcctgtgg aaaaacaata
    15781 cttattaaac tccaatatac aatacttcca aagtaatctc tggcgtttca tttcaccatg
    15841 caatcgcata actatttgtt ttttatcacc ttttacatta ttctcaccac aaattgcgta
    15901 tgaatttaca tacaaaaaag cgttcaattg atggcaattt aaaaaattca agaaggtatt
    15961 ttatccacaa aagctgtgga agatctctgc ataaatctgt ttataacccg accaaatccg
    16021 ccatctacgc ccctctcagc aaaacgcctt aatagcaggc actttttctc aagaaccttg
    16081 aaatcccagt tcaagatttc gattcaaatt tcaccacgtt ttacaagagc cgcattccaa
    16141 ccgtatcagc gaattgccgt cccttttgcg ttaattgcac acctgtgtca gtctgaaaaa
    16201 gataatcctc tttcaccata gcttcaaaag atgttgccca tacttcgttt tgagatgcct
    16261 gttgaatcca ctcaattggc acaccctcaa gcagacgcaa tccgaacaca atacgttctt
    16321 tatcttgctg agcccgtgac aacagttctc tgttggcaat gggcagctgc ccggccctca
    16381 ggcgcttcga atactgttcg atactggaca cattgccata gcggctttct aggacatatg
    16441 attgggcact cgggcctaat cccaaatatt ccaatcccct ccaataccga agattatgtt
    16501 gacaggcata ccctggcttc gcccaatttg aaagttcgta tcgagacaac tgctgcggct
    16561 ccaggtgggt ttcagcctgc tgcagaaaac acgcctcatg atccgggtcc atcacctcaa
    16621 atagcccgcg ccggaattcc gtataaaatc gagtcccttt ttcaagtgaa agggcataca
    16681 aggacaaatg agaaggatcc agctccagag cctgggtcag agtcatttcc caggactcca
    16741 ctgtttggct cgggatcccg tagatgaggt caagattgat gttggcgaat ccggcctgtt
    16801 tcgctatccg tacgccggat atggcttccg ccatgcttcc tgaaagtccc aacgtcgtcc
    16861 gttcctgtgg atcaaaagat tggactccta tactcacacg agtcacgccc gcctgccgaa
    16921 ggaaatcagc atactgatcc gtaagagatt ccggtgtcgc ttcgacggta atttcacact
    16981 gtttcgagag tgaaaacccg gatctaatac tggaaaagat tgtggccaac tgaccaggac
    17041 gtaagactgt gggagtcccc cccccgatat acaccgtaga cacggttcgc tcaagcattc
    17101 cgggttggct cgcatacagc gcaatttctc gttcaatggc ctgtaagaat tgtttcaccc
    17161 gttgctggtc atggaggaca agataaaagg cacaaaaatg acatcgtttc ttacaaaaag
    17221 gaacgtggag ataaatacca agatcggcgg taaacccatc gtgttcccct gagtgctcac
    17281 gtttctgttc cataacactc atctcatctt gcactccatc taccattcaa ccaattccac
    17341 ccgagaacca tcgcctggaa catgaaggac ggggataaat aaagaacgcg attcccaacc
    17401 cggaaaggtg aagggacaaa tatgtcaatt ggaaatccac accctatttt ccgtcatgat
    17461 ggcgttctcg atagacagga cacaccccct tgcactgaag aaggctggtg acctccgcaa
    17521 ccatttttcg attgccacag acatacaccg ccagatggtc cacatgcgga aactcctgga
    17581 gcaagtgcgt cacgcgtcct ctcttccccc tccaatgatg cgaaggttga gacagagtca
    17641 ggataaaaga aaaattctta tagcaatcag cccacttttg caactcgtcc aaatagtaga
    17701 tatccgactc actccgaagt ccccacagca gggtcacggg ctgagaacaa gattgctcga
    17761 agagactgga cagcatagac cataggggcg caatgccggt tcccgttccg acaaataaca
    17821 gatctcgttc agggtcttca tgaaggtgaa atgacccgaa aggaccggaa cagtcaagtt
    17881 cttcaccaac cgaatgctcc aacgcaaacg tcgcgccctt tcctttttct ccggcgttaa
    17941 agagtagtat gagttgatga gaggaactgg gaagtgaagc gaccgaatag tatctcaggt
    18001 atgaaaggcc cgacgctcca tcagggatgg tcacggcaat tgattgaccc gggagaaatt
    18061 cgaaggtcgg ggtatccact aactccaaga tcaattctcg aacatcttga gtcaaatgcc
    18121 gaatgcgaag aaccttggcc cggaatcgtc tgggggtcat gcctaggaat gtagagaatc
    18181 ttgtgtgatc tggcaagagc ctaacataaa ggttttctcc attcccgcca aaggcacaag
    18241 agcccttcac agatagcgcc gcgccgcatc tcgaatgaaa agaggacagc tcccatcaga
    18301 gcagagaaac caacactggg atgctcctga cgctcgcttt gactcacttg gggaaatggt
    18361 gtctggggct tcagacgaat gcgctggcca acgagattcc tgggaaaaag tggggacctc
    18421 ataggtcaac ggaaccggcg agatccaata aaaatgcata gatcatcgcc gtttcctcat
    18481 aacgtttaaa ccggcctgag gctccgctat ggcctgcatc catattagtt ctgagcaaaa
    18541 ggcgttgatt atcggtcttc aacgcacgca gtttcgccac ccattttgtc ggctcccaga
    18601 attgcacttg tgagtcgtgc aaacctgaag tcaccaaaag gtgaggatac gactggggtt
    18661 gaagattatc gtaaggcgaa taggaaagca tgtactcata aaactgttgt tggttgggat
    18721 ccccccattc atcatattct ccggtcgtca gggggatatt gggatctaac atcgtggtca
    18781 ccacatcgac aaacggaacc tgggccacca caccataaaa caaatcaggc cgcatattca
    18841 taatcgctcc catcaacaac ccaccagcac ttcccccgag agcgaataat ttttgaggtg
    18901 cggcgtaccc ttgctgaata agaaattcgg cacaggcgat aaaatccgtg aaggtattct
    18961 tcttgtacaa tagcttgccg ttttcatacc aatgtcggcc cagttcctcc ccacctcgaa
    19021 tgtgggctaa cgcatagaca aacccacgat ccagcaaact gagacggggg gagctaaaac
    19081 ttgcatccat gctcgcacca taggaaccat agccatacaa taacagggaa tgcgtgccat
    19141 tcggggcaaa tccttttcgg tagaccaggg atataggaat ggagatacca tctgatgctg
    19201 gagcgaaaag tcgttccgtc tggtagtggc taatgtgaaa attcccgaga accggctctt
    19261 gtttgagaag aattttttcc cgtgtttcca tgtgataatc atagatagtc atgggggtgg
    19321 tcatcgaggt atatccatac cgtagataag aagtatcggc ttcccaatta tcccccaagg
    19381 ttgccaggta agccggctct ccaaaatcca gttcatgctc agagccatcc gtggtccgaa
    19441 tcattcgaag gtggatgagc ccctgtcttc gttcttctag caccaggaaa ttctcaaaca
    19501 gctcgaatcc ttctaaaaat acttccgggc ggtgaggaat aacttcttcc cactgtgcga
    19561 ggttcgtctt atttgtttca gtccgcatca aacgaaaatt tttggcctca tcattgcttc
    19621 gaataaaaaa atgatcgcca agatgatcca catcgtattc atgtccgcgt tttcgtgcct
    19681 ggaatattcg aaaatcgctt tccggatgat ccgcatttac ataccgatac tcactcgtga
    19741 tggactgatg cgatccgatc atcaggtaac gtttggattt tgttttttcg atagaaaccg
    19801 aaaaggtctc atctgtttct tcatagacca atgtatccag ctccggattc gtccccaacc
    19861 gatgacgaaa gatctggttc gatcgcaacg tttcaggatc ctgtctggaa taaatgaggg
    19921 tacgattgtc atttccccac tccatattcc ctgtcaccaa aggaatgcat tcatcaattg
    19981 tctcgcccgt ggtcagattt ttaaagccaa tggtaaatat acgccgtcct tgggtatcaa
    20041 tcgcataggc tagaatatct tggccaggac ttatcgccca attccccaaa gaaaagtaat
    20101 catgcccccg agccaattcg ttgccatcaa ccatgatgga ttcgggattc gttaaggatt
    20161 tctctttccg gcagtagagg gcatactcct ttccctcttc atatcgaatg taataccaaa
    20221 aatcgccaag ccgaaacgga acagataaat ccgttggttg aatgcggcca caaatttctt
    20281 caaataaggc ctgctccaaa tccctggtat ggctcctcaa cgtggcggca tattcatttt
    20341 cagcctgcaa ataggccagg acttcaggat tctcacgatc tctaagccaa aaatactcgt
    20401 cc
//
LOCUS       DATIAL010000005         7288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1184124, whole genome shotgun sequence.
ACCESSION   DATIAL010000005 DATIAL010000000
VERSION     DATIAL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184124"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VLA60_00160"
     CDS             <1..327
                     /locus_tag="VLA60_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07790.1"
                     /translation="QRFLGPLDQAGFQYPWMHPDERMDQLHKAVSAIVETSAKEEEDA
                     MVTFDRIRASADKIAGRPAEISTRTRMGRPDRPKPPRLTESWFCCAEPTTQQFKPLLP
                     KGHGEI"
     gene            456..1154
                     /locus_tag="VLA60_00165"
     CDS             456..1154
                     /locus_tag="VLA60_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HSF07791.1"
                     /translation="MVSELKIQGTKLQELLGLKIPAVAIAFRDTAPAGIPRVTTPAPA
                     GCGYWKLAAEGQTFYTEGSDHFGCPVGAHTHGVELPPRVATELNGLVQTMVDMQYLTM
                     KEIPMIPHRQETFHVAVYAPLTQATFTPDVVLVRGTVKQLMLLAEATQAAGVAGGSAT
                     MGRPTCAVLPESLQSGHAATSFGCIGNRVYTGLGDDEGYYAIPGARVADIVGKLAVIT
                     EANRQLEAFHRSRL"
     gene            1296..2585
                     /locus_tag="VLA60_00170"
     CDS             1296..2585
                     /locus_tag="VLA60_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSF07792.1"
                     /translation="MPRLLLLLPTTTYRAEAFLEAARRVQVSITIGTERVPEGLLVAS
                     DNVLPLDVRHPQAAAQTVIEFVQHHPIDAVIGVDDVTAVTAATIAEAVGLPHNSVSSV
                     TAARNKRQMRELLSRQGIPVPSHKVFPLDGVARAFAKQVVYPCVVKPLILSASCGVIR
                     ANDEQDFENAFRRVGTLLINLNLAAVDEQARWILVEDFVPGIEVALEGLLTKGAFQPL
                     AMFDKPDPLDGPYFEETLYITPSRLSSTRQQQVVDCADRTAKALGLQEGPVHGEFRLN
                     ESGVWVIEMAARAIGGRCSRTLDFSAGISLEELILRHALQMPIPPLLRQEQAAGVMML
                     PIPYSGMLHEVRGQTEAKAVTGIVELTITAEPGEALIPLPEGTRYLGFLLARGKTPED
                     VERSLREAHQLLTVVISASPPTTDISPSSPHSKAMRF"
     gene            complement(2839..3273)
                     /locus_tag="VLA60_00175"
     CDS             complement(2839..3273)
                     /locus_tag="VLA60_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07793.1"
                     /translation="MGEVDTSPAVAAKVMEDLTAVDVDPEKCQRLYKAALIQSNTGVT
                     YRMLSRILETGKVDIVHYACDLDAEGKPTTKWKIRRILEQAPERFDKEVEAIKKAIID
                     DGETVKGAWVHDMTALPDVPAQGKSLDEWSRSMTTEVQKKPS"
     gene            complement(3532..4038)
                     /locus_tag="VLA60_00180"
     CDS             complement(3532..4038)
                     /locus_tag="VLA60_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF07794.1"
                     /translation="MNYSSPATANAMDEETASQRLGQSQRDIRANRQCLLVVDDDPSV
                     VGLLSQALQEWGYEVIVAGNGWEALVLAGRQSVDGMLVDIDMPIMDGRTMLSELRGLG
                     HRMPVLMMSGESDEPIFRQLLMEGAQGFFLKPPHLQSLQETCRQVFARYALEEYAASY
                     FPAGSNKE"
     gene            5146..6087
                     /locus_tag="VLA60_00185"
     CDS             5146..6087
                     /locus_tag="VLA60_00185"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HSF07795.1"
                     /translation="MSKKPQFPDEQSSEGAFTRQGDAFREWVTTDDSSGFPAARGRYH
                     LYVSWACPWAHRTIIVRKLKRLEDVIGMTVVDPIRDERGWAFRDGPGHSTDPINGFQF
                     LSEAYRATDPAYRGRVTVPVLWDRETRRIVSNSDDDIMRMFNSAFNQFTVSTLDLYPV
                     DLRQEIDQLNNGIYEQVNDGVYRAGFATSQRAYEHAVRHLFDMLDRLESRLTTRRYLF
                     GRSPMETDWRLFVTLIRFDAVYHGHFKCNIRRIVDFPNLFGYLKDLYQYDDVADTVNM
                     DHIKRHYYMTHDDINPTGIVPLGPDQDLRAPHGREHL"
     gene            6726..>7288
                     /locus_tag="VLA60_00190"
     CDS             6726..>7288
                     /locus_tag="VLA60_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSF07796.1"
                     /translation="MTLTAHNNVPLPSLMYGTAWKEGATTHLVQLAVASGFTAIDTAN
                     QLIHYREDLVGEALLALDKQGVKREALFLQTKFTPVNGQDHRTPYDPSADLTTQVQQS
                     FASSLTHLHTDYVDSYVLHGPYSRFGLGESDREVWAAMEELYQSGKTKMIGISNVTAG
                     QLTQLCAEARIKPMVVQNRCYASHGWD"
BASE COUNT         1803 a         1927 c         1894 g         1664 t
ORIGIN      
        1 cagaggtttc tcggtccact ggatcaggcc ggctttcagt atccatggat gcatccggat
       61 gagcggatgg atcaactaca taaggctgtg agtgcaatcg tggaaacgag tgccaaggaa
      121 gaggaagatg cgatggtaac ctttgatcgt attcgagcga gtgctgataa gatagccggg
      181 cggcccgctg agatttcgac tcggacacga atgggcaggc ctgatcgtcc gaagccgccc
      241 cggttaacgg aatcatggtt ctgctgcgct gagccgacga cccagcaatt caaaccgtta
      301 ctgccaaagg ggcatgggga aatctaatag gaggcaacat tgacgagcct ctgtgaagtt
      361 gatccataca ggttttcttg gtcaaactag gcggacacca agcccgaaac attggctggg
      421 tagaggcact taaatgtttg agagggggat gagaagtggt atctgaactc aaaattcaag
      481 gcacgaagtt acaggaactt ttaggattga aaatcccggc agtggccatc gcgtttcggg
      541 acacggcacc tgcgggcatt ccacgggtca ccactcccgc accggcagga tgcggctatt
      601 ggaagctcgc ggcagagggc cagaccttct atacggaagg atccgatcac tttggctgtc
      661 cggtcggagc ccatacccac ggtgtggagc tgccacccag ggtcgcgact gagctgaacg
      721 gtctggtaca aacgatggtc gacatgcagt acctcacgat gaaagaaatt ccaatgatac
      781 cgcatcgaca ggagaccttt cacgtggccg tctatgcgcc actcacacag gccacgttca
      841 ctccagacgt ggtgctggtt cgagggacgg tcaagcagct aatgctcctg gcagaagcga
      901 cacaggcagc gggcgtagcc ggcggtagtg cgaccatggg acggcccacc tgcgccgtgc
      961 tgccggaatc actccaatcg ggacatgcgg cgaccagttt tggctgtatt ggaaaccgcg
     1021 tctataccgg actcggggat gatgagggct attacgccat tccaggtgcc agggtggccg
     1081 acatcgtggg taagctggca gtgatcaccg aggccaatcg ccaattggag gcgtttcatc
     1141 gatcccgctt gtaaataatc ctcccaactg cctcctgaca gaaagttgga aaacaataga
     1201 accgtgtaat cgtaccctct cttgatatgt tgaggatctg aacgatgcaa acacgaagct
     1261 cgcggatttt tccagcctcc tgacaagaaa tcacgatgcc aagactcctc ctgctcttgc
     1321 cgaccaccac ctatcgagca gaggccttcc tcgaagcagc ccggcgggtg caggtatcca
     1381 taaccattgg gacagagcgg gttccggaag gccttcttgt cgcgtcggac aacgtgttgc
     1441 cacttgatgt tcgacatcca caggcggccg cccagaccgt gattgaattc gtccaacatc
     1501 acccgatcga tgcggtgatt ggagtcgacg acgtgaccgc cgtcacggct gctaccatcg
     1561 cggaggcagt cggattaccc cataactctg tctcgtccgt caccgccgcc agaaacaagc
     1621 ggcagatgcg agaattattg tcccgacagg gtattcctgt tccttcccat aaggtattcc
     1681 ccctcgacgg tgttgcaaga gccttcgcaa agcaggtggt ctatccctgc gtggtgaagc
     1741 ctctcattct ttccgccagt tgtggggtga ttcgtgccaa tgacgaacag gattttgaga
     1801 atgcgttccg tcgggtgggc accctcctca tcaacttaaa tctggcagcg gtggacgagc
     1861 aggcccgttg gatcctcgtc gaagattttg taccgggaat cgaagtggcg ttggagggtt
     1921 tgctcacaaa gggagccttc cagcccctgg cgatgtttga taagcctgat cccctcgacg
     1981 gcccctattt cgaagagacg ctctatatca cgccttcccg tctctcctcc acccgacaac
     2041 agcaagttgt cgactgcgca gatcgaacgg caaaggccct cggactacag gaagggccgg
     2101 tgcatgggga gttccgcttg aacgagagcg gggtatgggt cattgagatg gcggcacgag
     2161 ccattggcgg gcgatgttcc agaacgctgg acttttcagc aggtatatcg ctcgaagaac
     2221 tgatccttcg ccacgcactc cagatgccga ttcctcctct cctccggcaa gagcaggcgg
     2281 cgggagtgat gatgcttccg attccgtata gcgggatgct gcatgaagtg agagggcaga
     2341 ctgaagccaa ggcggtaaca ggaattgtcg aactgacgat aacggcagaa cctggagagg
     2401 cactcattcc gcttccggaa gggacgcgct acttgggctt tctcttagca cggggcaaga
     2461 caccggagga tgtggagcgt tcgcttcgtg aggctcacca gctattgacg gtcgtcataa
     2521 gcgcctcacc accgacaacc gacatatcgc catcatcccc tcactcaaaa gccatgagat
     2581 tttgaatgca ccaaccaggc tgcgacaacc tggcgctctc agcagactgt tgaaaatgca
     2641 aaattcttct gagagcttat caagatccaa aattttacgg ttttccatga gcgaagcagg
     2701 atgttcaaaa aggcccgtcc tgccctgagg gtgtctcgaa aggaaggccg tagccgcttg
     2761 gacgggcgga gcgtaagaga agtacctgag cacgaccaag gggcgagaac gccgttgggc
     2821 ggcttgttca acaccctgct agcttggttt cttctgcacc tctgtggtca tgctccgcga
     2881 ccactcatcc aggctcttcc cctgtgccgg cacgtccggg agcgcggtca tatcgtgcac
     2941 ccatgcgccc ttcacggtct caccatcgtc gataatggcc ttcttgatgg cttcaacctc
     3001 cttgtcgaac cgttcggggg cttgctcaag aatacgcctg atcttccact tcgtcgttgg
     3061 cttaccttct gcatccaagt cacaggcata gtggacgata tccacttttc cggtctcaag
     3121 gatcctggac aacatccgat acgtcacgcc tgtattcgac tgaataagcg cggccttata
     3181 gagacgctga catttctccg gatccacatc taccgccgtg agatcctcca tgaccttggc
     3241 ggcaactgct ggcgacgtat caacttctcc catagccgta ttcctccctg caagggttta
     3301 agcctgttgg caaaacacca gtcttgaacc taccagacaa ttccgaaaca gagcaagacg
     3361 aaggccaccg gtgactgcag acagggctcc gtctaactgc gaagcgaaaa atcagattaa
     3421 agaaataaac cgaggctact tggcaagcca cgaacgggga gggacaaact cagatatggt
     3481 gggcaccggg caaggaagat ttagaaaaaa aagcctctca atcagacttt ttcattcctt
     3541 gttcgagccg gcggggaaat aagaagcggc atattcctca agtgcatacc ttgcaaatac
     3601 ttgtctgcaa gtttcctgaa gagattggag atgaggtggc ttcaaaaaga agccttgggc
     3661 tccttccatc aacagttgac gaaagattgg ttcgtccgac tcacctgaca tcatcagcac
     3721 cggcatccga tgccccaacc cgcgtaactc acttagcatg gtccgcccgt ccataatggg
     3781 catgtctata tctacaagca tgccatccac cgattggcga cccgccagaa ccaaggcttc
     3841 ccatccattt ccggcaacaa tgacctcgta tccccattcc tgaagcgcct gacttaacag
     3901 accgaccaca gatggatcat catcgaccac caacagacat tggcgatttg cccttatatc
     3961 cctttgggat tgaccgagcc tttgagacgc ggtttcctcg tccattgcat tcgctgttgc
     4021 gggagatgaa tagttcataa ccaatcctca actttcaaaa tgggttaaca ttttaaacca
     4081 tcgtgtcaag catcatttat cctaaaaatt taaggataac tttgagtaaa gattactctc
     4141 aaaaaccctg gttaattgga gagaaagccc ttggtttatg gtttcacctc atttgcccta
     4201 aggaaagact ggtaggatct gtattggttc cccttagaat agcaaggaga catgttgggg
     4261 tctgttcaaa attgcacacc aatatccctg acttatcacc ctttcaattc aacaagtcct
     4321 gcaaatggcc aggcaaaaat cattaactcg aatcctaaat caaaaactcc taatatctcc
     4381 taaagcatca atttagatat aaaaaatgaa atctcgatac atcaattcca ggaagaattt
     4441 atgtgataca aactacaagg aaaacaaccg gaaatttttc catggagagg taaagggcat
     4501 tcttgaagag cgccctcctt tgaaggaata gccagggctc tttaatcggg cacagatccg
     4561 tggccaggtg tcgatgaata ttgatcccag gaccaaagcc cgggattcat ttggaacggg
     4621 cacgatgaag cttccagaca cacacgcagt aattcattcc tggtggttct caagagcctg
     4681 gcggaggcat gcaatggaga attcgcctgg attcggggaa acggcagatt ccccgggatg
     4741 tccggcccca ccagatttca gtccagcatg gaaatcgagg ggaccgaatc aggagccatc
     4801 cacggtaagg ccacatggcc ggaattcacc tagcaattac cctgacatgc cagcctacgc
     4861 cccatattct caatcgcctg aaccaattta tggtgtgcca taaactctag ccttattctc
     4921 atcaaattgc atgttttttt aaaaatttaa atcgtgcaaa gtcctgctga gtcgattctg
     4981 gcaggtccta gatccactct cgatgagatg aatgtcattg ccacccatga tagagtgggg
     5041 agaaattttg agctccgaag ttattgctcc cgggaagcca ccaagaagag aacaccacgc
     5101 ccgctgaggc atcttaagaa tcatgatgaa gtcattgagg atccgatgag taaaaaacca
     5161 caatttcctg acgagcaatc cagcgaaggc gcttttacac ggcaaggcga tgcatttcgg
     5221 gaatgggtga cgacagacga cagttcgggg tttcctgcgg cgcgtgggcg gtatcatctg
     5281 tatgtctctt gggcatgtcc gtgggcgcat cgcacaatta ttgtgcgaaa gctcaagcga
     5341 ttggaggacg tgattggcat gacggtggtt gatccgattc gggatgagcg gggttgggcc
     5401 tttcgagacg gccctggaca ttcgaccgat cctatcaatg ggtttcagtt tctgagtgag
     5461 gcttatcgtg cgaccgatcc ggcctatcgc ggacgcgtga ccgttcccgt gttgtgggat
     5521 cgtgaaaccc ggcgtatcgt gagtaattcc gatgacgaca tcatgcggat gttcaattcg
     5581 gcctttaatc aattcacagt cagtacgtta gacctttatc cggttgatct ccggcaggag
     5641 attgaccaac tgaataacgg tatttatgaa caggtcaatg atggggtcta ccgcgcaggg
     5701 tttgcgacat cccaacgggc ttatgaacac gcagtccggc acctgtttga catgctggat
     5761 aggctggaat cccgcttaac cacacgacgg tatctctttg gcaggagccc gatggaaaca
     5821 gactggcggt tgttcgtgac gctcatcaga tttgacgcgg tgtatcacgg ccattttaaa
     5881 tgcaatatcc ggcgcattgt ggattttccc aatttgtttg gctatctcaa agatctctat
     5941 caatacgatg acgtggcgga caccgtgaat atggatcaca tcaaacggca ttactacatg
     6001 actcacgatg acattaaccc cacgggtatc gttcccctcg gccccgatca ggatcttcgc
     6061 gctccccatg gccgggaaca tctgtagaat gccaaccggc atttttagta cgtcttgaag
     6121 aaatttttct actgcggtag ccaatccgag actcccaacg gttacaaagc ctgcttcctc
     6181 tccatttttc ataataccgt ccggatgcct gaaaattacc agagaagaag ttagaatatg
     6241 ggccaaaatt tcaatcggca cttattattg ccactggaca aggtgaatcc aaagcaggta
     6301 cgtgcttaaa tcgaatccta cagcatttat ggttcctcgc tcgttcaagt ttatctctga
     6361 aaggttcgtt ttgatccgac gcctgccgtc tgactcaagg acgggacgct ctcatccgtt
     6421 ggcgggcctt gtatgagcgg agcgagttgg tccgccctcc tcaggttggc gtccgggcct
     6481 tcccatgagg acagacgggg cgtcactggt tttggggcct tttgccgaaa caaaagggcc
     6541 tcgcctgccg gggcaaaacc cggcaacatt gaacatccga ttgacacgag agttgggaac
     6601 acatgtgcga ggttttcccc tattagcgat tgtttacgag gaaaccccaa gatggatttc
     6661 cgatacagat cgtcaagcta taaggcaaga accataaatt ctgtattaaa tctggaggaa
     6721 tcgttatgac attgaccgca cataataatg tcccccttcc ctcgttgatg tatggcacgg
     6781 cgtggaaaga aggcgccacg acccatctgg tgcaattggc ggtggcttcg gggttcacgg
     6841 ccatcgacac ggccaaccag ctcattcatt accgggaaga cctggttggt gaagctctgc
     6901 tggccctcga caagcaagga gtgaagcggg aggccctgtt tttgcagacg aaatttacgc
     6961 cggttaacgg acaggaccat cgtacgccct atgatccctc ggccgatctc accactcagg
     7021 tgcagcaatc ttttgccagc tcgttgaccc atctccacac cgactatgtg gactcatatg
     7081 tcttgcacgg gccctattca cgattcggat taggagaatc ggaccgggaa gtgtgggcgg
     7141 ccatggagga gctctatcaa tccgggaaga ctaaaatgat tggcatcagt aatgtcacgg
     7201 ccggacaact cacccaactc tgtgcggaag cgagaatcaa acccatggtc gtgcagaacc
     7261 ggtgttatgc ctcccatgga tgggatag
//
LOCUS       DATIAL010000006         3938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_754121, whole genome shotgun sequence.
ACCESSION   DATIAL010000006 DATIAL010000000
VERSION     DATIAL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754121"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /gene="flgG"
                     /locus_tag="VLA60_00195"
     CDS             complement(<1..648)
                     /gene="flgG"
                     /locus_tag="VLA60_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HSF07797.1"
                     /translation="MIRAMHTASTGMSAQQLNIDTIANNLANVNTTGFKRSRAEFADL
                     LYQISRLPGASASNVGVFPVGMQVGLGVRPVTVAKEYVQGSLKQTSNELDLAIEGVGF
                     FQVTRPDGTTMYTRAGSFKRDSTGNVVTGDGDQLIPSITIPSGALKVDIGQDGTVSAL
                     LPGVSQATQVGQIQLVRFDNPSGLISQGNNLFSESTSSGPAQQGTPGFSTGFGNIQ"
     gene            complement(744..1463)
                     /locus_tag="VLA60_00200"
     CDS             complement(744..1463)
                     /locus_tag="VLA60_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HSF07798.1"
                     /translation="MNRGIYPPLAGAITLEHRMEVLSHNIGNVHTTGFKKDKPVFATI
                     LGQTSGPSVAGIDLFPVMDSLPPDRSQGVLYQTGESLDVGLEGEGFLVAQTADGLRYY
                     RGGKLHRNADGNLVAHTGDPIMGKKGPIALPMGEVVIGNDGTLRVNNKIIDQLRVDKI
                     LIGQEMTKVGNLFWTVQGNGVADKVTTVHQGMLEKSNVNPSLDMVELIKVTREYEQMQ
                     KAIKAMDDLAAQAIQAGRVQG"
     gene            complement(1793..2533)
                     /locus_tag="VLA60_00205"
     CDS             complement(1793..2533)
                     /locus_tag="VLA60_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HSF07799.1"
                     /translation="MKKKHEEHENHERWLVSYADFITLLFAFFVVMYSVSSVNEGKFR
                     VLSDSLVSSFTNNKPVGDLSLVNLPIQKSTPIQVKEEQKSSDNSRSYLKVANAIIAAK
                     VPQGVKITSTERGLNIRIKDEALFSSGSAIINPQIEEFIDLVASLVKDLPNLIAVEGH
                     TDNQPIRSPVYPSNWDLSTARANALIRYLIGHHQLAAQRFSSTGYAGTRPVVSNETPE
                     GQASNRRVELIVLRDINPDTSPSHPYLP"
     gene            complement(2573..3334)
                     /locus_tag="VLA60_00210"
     CDS             complement(2573..3334)
                     /locus_tag="VLA60_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HSF07800.1"
                     /translation="MDILSILGIILSIVAIVGGQHLEGGHLSSIMQFTAFLIVMGGTL
                     GAVMLNYPMPIFLKALSSLSMVFGNVHVDMKGIIAKIVELSNISRKQGLLALEGQMKT
                     IQDPLMAKGVQLVVDGTEPPKIREILEVEVEIFEEEFQLAGKVYESLGGYAPTVGIIG
                     AVLGLIHVMENLADPSKLGSGIAVAFVATVYGVGAANLFFLPMGGKIKMKAKELVMGK
                     LMVIEGLISVAQGENPRMIEEKLSGFLPTSEKKKK"
     gene            complement(3350..3838)
                     /locus_tag="VLA60_00215"
     CDS             complement(3350..3838)
                     /locus_tag="VLA60_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSF07801.1"
                     /translation="MSNMISKTLFHGDMEEGNGIVHQLVSFQLGNEIFAIDILGVQEI
                     IRLVEITPVPNAPHYVDGVVNLRGKVIPIINLRVRFGLSATEPTKDTRIVVVEVAHLI
                     LGFIVDSVEEVLRLPEDCIEPPPPTGRGRTDECHRGVGRVNGNLVLLLDLELLFGNRT
                     SS"
BASE COUNT         1056 a         1028 c          783 g         1071 t
ORIGIN      
        1 ctgaatattc ccaaatcctg ttgaaaatcc cggggtccct tgttgtgccg gacccgagga
       61 agtgctctcc gaaaacaaat tattaccttg tgaaatcaac ccggacgggt tatcaaaacg
      121 caccaattga atttgcccta cttgggtggc ttgggacacg ccgggtaaaa gggccgaaac
      181 cgttccgtct tgaccaatat caactttaag cgctcccgaa ggtatcgtaa tgcttggtat
      241 aagctgatcg ccatctcccg tgacgacatt gcccgtacta tcgcgtttaa aagatccggc
      301 acgggtatac atcgtggttc catccggacg cgtgacctgg aaaaatccaa ccccttcaat
      361 ggctaaatcc aattcattag aggtctgctt gagacttcct tgcacatatt ccttggccac
      421 ggtaacgggc cttacgccta aaccgacctg cataccgaca gggaagactc cgacattcga
      481 ggcactggct cccggaagac gggaaatttg ataaagaaga tcggcaaatt ccgcccgact
      541 tcgcttaaac cctgtcgtat tcacattggc cagattattc gcaatggtgt caatattaag
      601 ttgttgggca gacatgcccg tcgaagcggt atgcatcgct cgaatcatcg tgatcgctct
      661 cccttcttca ccttacgcca ttgccccgaa aattcctacc atatggaaac atcccattat
      721 ccgttacaca tgcctcttca gattcaacct tgaactcgac cggcttgaat ggcctgagcc
      781 gccagatcat ccatggcctt aatggctttc tgcatttgtt cgtactctct tgtcactttg
      841 attaattcaa ccatatcaag agaaggattc acgttcgatt tttccaacat accctgatgc
      901 actgtggtca ctttatcagc aacaccattc ccctgaacgg tccagaaaag attgcccacc
      961 ttggtcattt cctgcccaat gagaattttg tccacacgca gttggtcaat gatcttatta
     1021 tttacccgaa gagttccgtc attgccgatc acgacctcac ccatcggcag agcaataggc
     1081 cccttttttc ccatgatggg atcacccgta tgagcgacta aatttccatc agcatttcta
     1141 tgtaattttc caccgcggta atagcgtaat ccatctgccg tttgagcgac tagaaaccct
     1201 tcaccttcca atcccacatc taacgattcc cctgtctgat ataacacccc ttgcgagcga
     1261 tctggaggaa gcgaatccat cactgggaag agatctatgc cagccaccga aggtcctgag
     1321 gtttgtccta aaatggtggc gaacaccggc ttgtcctttt taaagcccgt cgtatgcaca
     1381 ttcccaatat tatgagaaag gacttccatg cggtgctcaa gcgtaatcgc ccccgctaag
     1441 ggcggataga tgcctctgtt cattttctcc tcctagacct aaataaggaa ggaattctgc
     1501 aacgcatgtg ccaataagat gttttggaat ttttcatcaa atgaatgtcc acagacgcag
     1561 cgttagtcaa ctgattggat tctttcaccc gcatacttct acttgtttta cataacagga
     1621 acgtttggtg tatgagacgt tttggaatcg agagcggaga cccgttttcc catcgccgga
     1681 taattaccat accggcatta ctttcccata gacccgatcc tgcctagtga atgactgaaa
     1741 caaaattaat cggcgtgccg attgttattc gtatttacgt tacgaaatag atctaaggca
     1801 gatagggatg ggatggagac gtgtccggat tgatgtcccg cagcacgatg agctctaccc
     1861 gacgattgga ggcttgcccc tcaggagtct cgttggatac tacaggtcgt gtacctgcat
     1921 aaccggttga ggaaaatcgc tgggccgcca attgatgatg tccgatcaaa taccggatta
     1981 acgcattggc tctggcggtt gagagatccc aatttgaagg atacaccgga gatcggatgg
     2041 gttgattgtc tgtatgccct tccacagcaa taagattagg caaatctttg accaaactgg
     2101 caaccaaatc gatgaattct tcgatttgcg gattaatgat ggcactccca ctgctaaata
     2161 atgcttcatc ctttattcga atattcaagc cccgttcggt cgaggtgatt ttgacgcctt
     2221 gagggacctt ggcagcgatg atggcattgg cgacctttaa gtaggatcgg ctgttatcag
     2281 aagatttctg ctcttctttc acctgtatcg gggtactttt ttgaatggga agatttacga
     2341 gtgaaagatc ccccactggt ttattattag taaatgaact cacaagggaa tcactgagca
     2401 cacggaactt tccttcattg accgaagata ccgaatacat caccacaaaa aatgcgaata
     2461 agagggtaat aaagtctgca tatgacacta accatcgttc gtgattttca tgctcttcgt
     2521 gttttttctt cacactattc cctgttcgac taaaccgtcg tccaccatca actcattttt
     2581 tctttttctc cgacgtgggt agaaacccgc ttaacttttc ctcaatcatt cggggattct
     2641 ccccttgagc gacggaaatg agtccttcaa tcaccatgag ttttcccatc accaattcct
     2701 tagctttcat tttgattttt cctcccatag gcaaaaaaaa caaattggca gctcccactc
     2761 cataaacagt ggccacaaac gccacggcaa tgccgctacc taactttgac gggtcagcta
     2821 aattttccat gacatggatt aaccccagaa ctgcaccaat gatcccgact gtaggggcat
     2881 aacctcctaa cgattcataa actttacccg ccaattgaaa ttcttcttca aatatttcaa
     2941 cttccacctc gagaatttcc cgaatttttg ggggttccgt cccatcaacg accaattgga
     3001 caccttttgc catgagggga tcttgaatag ttttcatctg cccttctaat gctagcaatc
     3061 cttgttttcg acttatattg gagagttcta cgattttagc aatgatgcct ttcatatcca
     3121 catgcacatt gccaaagacc atgctaagac tactcagtgc tttcagaaaa atcggcatcg
     3181 gataattgag catgactgct ccaagcgttc cgcccatcac aatgagaaag gcggtgaatt
     3241 gcatgattga actgagatgc ccgccctcta aatgttgtcc cccaacaata gctactatag
     3301 acaatataat gccaagaatt gacaaaatat ccataaccca ttgctctccc taactggatg
     3361 tccgattacc aaacaaaagt tcgagatcca gcaatagcac taagttccca tttacccgcc
     3421 ccactcctcg atgacattca tcagttcttc cccgtcccgt aggcggaggg ggttcaatac
     3481 agtcttccgg gagtcgcagg acttcttcca ctgaatccac aataaatccc aaaatcaaat
     3541 gagccacttc cactactaca atccgagtat ctttggtggg ttctgtagca gatagaccaa
     3601 aacgaacccg aagattgata atggggataa cttttccacg taaattcacc actccatcca
     3661 cataatgagg agcattcggc acaggggtga tttcaaccag ccgaataatt tcttgcacgc
     3721 ccagaatatc aatggcaaat atctcgtttc ccaactgaaa actcaccagt tgatgcacga
     3781 tgccattgcc ttcttccata tcaccgtgaa ataaagtctt cgatatcata ttcgacatga
     3841 gacatgtccc ttcagactca aaaagagtct cttcctgacc attctaacaa tttacgggtg
     3901 gatgcccatc ggaaatcgcc aatgcttctc atacataa
//
LOCUS       DATIAL010000007         4454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_324368, whole genome shotgun sequence.
ACCESSION   DATIAL010000007 DATIAL010000000
VERSION     DATIAL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324368"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VLA60_00220"
     CDS             <1..392
                     /locus_tag="VLA60_00220"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSF07802.1"
                     /translation="GYYANQENGSAISSGRNARWDGVAGYAIYDFNEQWGLRFRGEIW
                     EDAGGDQSCLGTAGSGGNANVCAVSAPIAQTLWENTVTLQYKPTPSLITRLEFRYDKS
                     NQNVFLDGRNTLTNNQQTLAVEAIFLF"
     gene            417..1730
                     /locus_tag="VLA60_00225"
     CDS             417..1730
                     /locus_tag="VLA60_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSF07803.1"
                     /translation="MKAYIDKASRAIFSTLMAVLVLNGLGSFVWAQDGGSSQLNAGDT
                     AWVLMSSALVLAMIIPGLALFYGGMVRSKNVLSTMMHSFIAVCLVSVVWVFWGYSLAF
                     APDVGGVIGGLNWLGLIGVGPEPQPGSTIPHLAFMVFQLMFAAITVALISGAIAERMK
                     FSAFALFAVLWVTFIYAPLAHWVWGGGWLAELGDLDFAGGTVVHISSGVAALACALVL
                     GKRHGYGTDNMSPHNLPFTVIGASLLWVGWFGFNAGSALAANGVAASAFVATHVATAA
                     GALAWVGVEGLYRGRATVLGAASGAVAGLVSITPAAGYVGPIGALVIGFGGGVVCYLG
                     VFLKHKLGYDDALDVLGIHGIGGTWGAVATGLFASMGGGSGLFFGNPSQVVIQVIGVG
                     ATWVFSFVGTYLILKLVDGTIGLRVTREEEVLGCDLTQHRERAYG"
     gene            1844..2182
                     /locus_tag="VLA60_00230"
     CDS             1844..2182
                     /locus_tag="VLA60_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSF07804.1"
                     /translation="MKLIQAIIKPFKLDEVKDALIELGIQGMTVTEVKGFGRQKGHKE
                     TYRGTEYQIEFVPKIKLEIALADDRVGEALEAIMRAAKTGSIGDGKIFVSELHSVIRI
                     RTGESGEGAV"
     gene            2227..3540
                     /locus_tag="VLA60_00235"
     CDS             2227..3540
                     /locus_tag="VLA60_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HSF07805.1"
                     /translation="MNVLIPRSTMGRLTGLVLGLSLSHPFAVWGGEEGLPKIDTGDTA
                     WLLMSSALVLCMTLPGLALFYGGLVRKKNVLGTIMHTMVILCVISLIWVLCGYSLAFG
                     PDVGGVIGSLAWVGLNGVGLDPSPQYASTVPHQVFMFFQLMFAAITVALITGSVAERM
                     KFKALLMFAVLWSLLIYTPLAHWVWGGGWLAKLGALDFAGGAVVHISSGFGALVCAVM
                     LGRRKGFGAEPMPPHDLPMTVLGAGLLWFGWFGFNAGSALGANGVAAAAFLATHTAAS
                     AGGLSWMVAEWVHRGKPTVLGVASGVVAGLATVTPAAGFIGPMSALLIGLVAGTVCYI
                     AVVWKMRLGYDDSLDVVGIHGVGGFMGILATGLFASIGAQGFFFGNPGQFGIQVVLAL
                     VTLAFSVIGTYFILKIVDVTVGLRVSAQDEEMGLDLSQHNERAYS"
     gene            3682..>4454
                     /locus_tag="VLA60_00240"
     CDS             3682..>4454
                     /locus_tag="VLA60_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HSF07806.1"
                     /translation="MTAKEVLDFAKKNKVEMVDVKFVDLMGTWQHFSIPTSELTLDLF
                     KEGSGFDGSSIRGWRMIQNSDMLVVPDPNTACMDPFMEIPTLSLIADVQDPITRTIYE
                     RDPRGVALRAEQYLKSTKIGDISYWGPEAEFFIFDHVSFDQNSHSSYYFVDSEEGIWN
                     SGKDGVNLGSRPRHKEGYFPVAPVDSQQDIRSEMCREMEKVGIKVEKHHHEVATAGQA
                     EIDLRFDTLVKMADQMMMYKYIVRNVARRHNKTVTFMPKP"
BASE COUNT          921 a          981 c         1406 g         1146 t
ORIGIN      
        1 aagggtatta tgccaatcag gaaaatggaa gtgccatcag ctcgggtcgc aatgcccgtt
       61 gggatggtgt cgcggggtat gccatttatg atttcaatga acaatggggt ctccgttttc
      121 gtggagaaat ttgggaggac gccggaggcg atcagagttg tttggggacg gccggatccg
      181 ggggaaatgc gaatgtctgt gccgtgtcag ctccgattgc acaaaccctt tgggagaaca
      241 cggtgaccct tcaatataag ccgacaccgt cgctgatcac ccgattggaa tttcgatatg
      301 acaaatccaa tcaaaatgtc tttttagatg gaagaaatac cctgaccaat aaccaacaga
      361 cactggcggt tgaagccatt ttcctctttt aagaagaggt cgaaggagca aagacgatga
      421 aggcgtatat tgacaaagcc agtagagcca tattttcgac attaatggct gtcttggtct
      481 tgaacggatt gggctcattc gtgtgggctc aagatggagg atcgtcgcaa ctcaatgccg
      541 gcgacacggc ttgggtgttg atgtcctcgg ccttggtgtt ggccatgatc atacccggat
      601 tggcgttgtt ttatggcggg atggtccgga gtaaaaatgt gttaagtacg atgatgcata
      661 gttttattgc cgtctgtctc gtcagtgtgg tgtgggtgtt ttggggctat agtctcgcgt
      721 ttgctccgga tgtgggaggg gtcattgggg gactcaactg gctgggattg ataggggtgg
      781 gacccgaacc gcagccaggt tccacgattc cccatttggc cttcatggtg tttcagctca
      841 tgtttgcggc gattaccgtg gcgctcatta gtggagccat tgccgagcga atgaaattta
      901 gtgcctttgc cttgtttgcc gtgttgtggg tgacattcat ttatgcgcct ctagctcatt
      961 gggtatgggg aggtggctgg ctggcggaat tgggtgactt ggattttgcc ggtgggacgg
     1021 tggtccatat cagttcggga gtggctgcgt tagcctgcgc gctagtcctg ggaaaacgcc
     1081 acggatatgg taccgacaat atgagccccc ataacctgcc gtttacggtc atcggggcca
     1141 gtttgttgtg ggtcgggtgg tttggattta acgcgggaag tgccttggcg gccaatgggg
     1201 ttgcggcgag tgcctttgtg gccactcatg tggcgacggc cgccggagcg ttagcctggg
     1261 tcggcgtgga gggactctat cgaggaaggg cgactgtgct gggcgcggcc agcggtgccg
     1321 tcgcgggtct ggtgtccatc acgccagccg ccggctatgt ggggcccatc ggggcgctgg
     1381 tgattggttt tggaggaggc gtggtgtgtt atctcggtgt atttttaaag cataaactag
     1441 gctatgatga tgccttggat gtgctaggca tccatggaat cggaggaacc tggggggccg
     1501 tggccaccgg attatttgcg agcatgggtg gaggctccgg gctctttttc ggaaacccct
     1561 cgcaggttgt cattcaagtc attggagtgg gcgcaacatg ggtgttctca tttgtcggca
     1621 cctatctcat cctcaaactc gttgacggca cgatcgggtt acgtgtcact cgagaagagg
     1681 aggtgctcgg atgtgatctg acccaacacc gggaacgggc ctatgggtaa ttcatacaaa
     1741 tgagctgtgt acgtttcaag agcaagggcg gtcgggaggg tcaggtaccc gcgacgacgg
     1801 acagcgattc cgagtgtctt aatccaatgg aaggatggtt cttatgaagc tgattcaagc
     1861 cattattaaa ccctttaagt tagatgaagt gaaagatgcg ttaatcgagt tgggtatcca
     1921 gggcatgact gtcaccgaag tgaagggatt tggtcgtcaa aaaggtcata aagagaccta
     1981 tcgaggcact gaatatcaaa ttgaattcgt tccaaaaatt aaactggaaa tcgccttggc
     2041 cgatgaccgg gtgggggagg ccctggaagc cattatgcgt gcagccaaaa ccggcagtat
     2101 tggggatgga aagattttcg tctccgagtt acatagcgtt atccgtattc gtacggggga
     2161 atcgggcgaa ggagcggtct gacccttggc acccgaccac atcacgtgtt tactcgagga
     2221 ggggaaatga atgtgttgat tccacgctca acgatgggaa gattgaccgg actggtgctt
     2281 ggcctgagtt tgagtcaccc ctttgctgtt tggggaggtg aagagggttt gccgaaaatt
     2341 gatacgggcg ataccgcctg gctcctcatg tcttcggcgc tggtgttgtg tatgacgctt
     2401 cccggtctgg ccttatttta cggcgggctg gttcgcaaaa aaaatgtcct cggaaccatc
     2461 atgcatacca tggtcattct gtgtgtcatt agcctgattt gggtgctgtg cggatatagt
     2521 ttagccttcg gtcctgatgt gggaggggtc attggcagcc tggcatgggt gggtctgaat
     2581 ggtgtgggat tggatccgag tccgcagtat gcgtcgacag tgccccatca agtctttatg
     2641 ttcttccagc tcatgttcgc cgccatcacc gtcgccctga ttaccggcag tgtggccgaa
     2701 cggatgaaat ttaaagcttt actcatgttt gccgtcttat ggtctcttct gatctatacc
     2761 ccgttggcgc attgggtgtg gggtggcggg tggttggcca aacttggcgc attggatttt
     2821 gccggaggtg cggtcgtcca tatctcttcc ggattcgggg ccctggtctg tgccgtgatg
     2881 cttgggaggc gcaaaggatt tggtgcagag ccgatgcctc cacatgattt gccgatgacc
     2941 gtcctgggtg cgggactcct ctggttcggg tggttcggct ttaatgcggg gagtgcgctt
     3001 ggagccaatg gcgtggccgc ggccgcgttt cttgccactc ataccgccgc ctcggccggg
     3061 ggactcagtt ggatggtggc cgaatgggtc catcgaggca aaccaaccgt gttaggggtc
     3121 gccagtggag tggtcgcggg tttggccacc gtgacgccgg ctgcaggctt tatcgggccg
     3181 atgtcggccc tcctgattgg tctggttgcc ggcaccgtgt gttatatcgc ggtcgtgtgg
     3241 aaaatgcggc tcggctatga tgattcgctt gatgtcgtgg gtatccatgg tgtgggcggg
     3301 tttatgggga tactcgcgac agggttgttt gcctccatcg gggcccaggg tttctttttt
     3361 ggaaacccgg gacagtttgg tatacaagtc gtattggctt tggtaacctt ggcgttttct
     3421 gtcatcggga cctatttcat tctgaaaatt gtggatgtga cagtggggct gagagtctcc
     3481 gcccaggacg aggaaatggg gttggatctc agtcaacaca acgaacgggc ctattcctaa
     3541 catggagatg ggtggtcgag catgatgggg tggaaccgga gttggcctcc gatccacgtc
     3601 ggatgagtgc atggtaacgg ttgaggcgat gagggggttt tttactttta taaacagttg
     3661 tgccggcgaa ggaggtattg aatgaccgcg aaagaagtgt tggattttgc gaagaagaac
     3721 aaggtggaaa tggtcgacgt gaagtttgtt gacctcatgg ggacctggca gcatttttcg
     3781 atcccgacct cagagttgac gttggatctc tttaaagagg gctccggttt tgatggatcg
     3841 tctattcgtg gctggcgcat gatccaaaat agcgacatgt tggtggtgcc tgacccgaat
     3901 accgcctgca tggatccatt tatggaaatt ccaacgttaa gcctgattgc cgacgtgcaa
     3961 gatccgatta cccggaccat ctacgagcgg gatccgcgtg gagtggcgct tcgagcggaa
     4021 caatacctca agagcacgaa aatcggagat atctcctatt ggggccctga agccgaattt
     4081 tttatttttg accatgtgtc gtttgatcaa aacagccact ccagttacta ctttgttgat
     4141 tcagaagagg gcatctggaa ttccggaaaa gacggagtca acctcgggtc ccgcccgcgc
     4201 cacaaggagg gctatttccc cgtggctccg gtcgactcgc aacaggatat ccggtcggaa
     4261 atgtgccggg aaatggaaaa agtcggaatc aaggtggaga agcatcatca tgaagtggcc
     4321 acagcaggtc aagccgaaat cgatcttcga ttcgataccc tggtcaagat ggccgatcag
     4381 atgatgatgt acaaatatat tgtgcgcaat gtcgcgcgtc ggcataacaa aaccgtgacc
     4441 ttcatgccca agcc
//
LOCUS       DATIAL010000008         8058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1292022, whole genome shotgun sequence.
ACCESSION   DATIAL010000008 DATIAL010000000
VERSION     DATIAL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292022"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VLA60_00245"
     CDS             complement(<1..417)
                     /locus_tag="VLA60_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07807.1"
                     /translation="MKISRGLPTVRPAPCPVLTIGNFDGQHLGHRALVQAVVDCARQI
                     NGYPMVLSFAPHPVEVLRPGSVHKFLSDDHEKIAFFERLGVGELVILPFTRELAGLRP
                     DEFVRQVLHDGLGIRKLFVGENFVFGKGRSGGVKDLI"
     gene            complement(414..1397)
                     /gene="hemB"
                     /locus_tag="VLA60_00250"
     CDS             complement(414..1397)
                     /gene="hemB"
                     /locus_tag="VLA60_00250"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HSF07808.1"
                     /translation="MGFPVHRLRRLRQHESLRRMVRETHLNPGDFIYPLFVTFGENKQ
                     EPIASMPGQWRWSVDRVVQEAKDAWESGVPAVILFGIPEFKDERGSSAYERDGIVQRA
                     VRALKDHLPDLMVITDVCIDEYTSHGHCGIVRDGRILNDETLECLQAMALSHVEAGVD
                     MVAPSDMMDGRVAAIRQALDQQGFSETPIMAYSAKYASALYAPFRDAAFSSPSFGDRR
                     SYQMDAANAREALREVELDIQEGADIVMVKPALFYLDILRRVRELVAVPVAAYQVSGE
                     YSMIKAGAQLGWVNEASVMMESLLAIKRAGADLILTYFAKEAACLLTRAGV"
     gene            complement(1406..1798)
                     /locus_tag="VLA60_00255"
     CDS             complement(1406..1798)
                     /locus_tag="VLA60_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF07809.1"
                     /translation="MVPVKSCMVPVDKIVKVDRDILVKTAAEMMRDNGIGSVIVTSGE
                     EIIGILTDTDLVRRVVAAGADPMRTTVEKIMSAPIVSIEADRTLLDANDLMAKEHIRH
                     LGVTQAGTMVGMISVRDLVVFLTNLPRK"
     gene            complement(1866..3437)
                     /gene="cobA"
                     /locus_tag="VLA60_00260"
     CDS             complement(1866..3437)
                     /gene="cobA"
                     /locus_tag="VLA60_00260"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HSF07810.1"
                     /translation="MTKSVANHGRVFLVGAGPGDAKLLTLRGKECLEQADVVLYDHLA
                     NPELLKFAPSHAECIYVGRKGRGAYRDQAEIHALLVTKAQEGKCVVRLKGGDPFVFGR
                     GGEEAEVIADAGIPFEVVPGVTAAVAVPAYAGIPVTHRTLASTVAFVTGHEDPTKPSS
                     AMEWPRLASAEGTLVFLMGMKNLPQIADRLIQEGKPASTPVAVIRWGTYARQQTVVGT
                     LSDIVGLASQADMSPPSVIVVGEVVRLRERLNWHESRPLFGQGVLVTRPRAQAPALSN
                     LLAAQGAEPVECPTLEIHPPDSWTPVDEAIAAISTYDWVIFTSVNGVQSFMERLWFHH
                     KDVRSLAGIRVCCIGPRTREEAARWGIASDLVPQDFQAEGILQALGGLGVARQRILIP
                     RAKVAREILPDQLKAMGATVHVVHAYQALPPIVDMGPLRDRLRNREIQYVTFTSSSTV
                     KNFCQLFEDRKELQEITRHVIVAVIGPITAQTVQEEGLSVDVMALENTVPALVDAIII
                     HAQQNPKDTRLVSSS"
     gene            complement(3434..4384)
                     /gene="hemC"
                     /locus_tag="VLA60_00265"
     CDS             complement(3434..4384)
                     /gene="hemC"
                     /locus_tag="VLA60_00265"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HSF07811.1"
                     /translation="MNQGKSRTRSTLIVGTRGSQLAIWQAEWVQSQLKALAPDISVIL
                     KRIQTSGDKIQDVPLAKIGGKGLFVKEIEEALLRRDIDLAVHSMKDVPSELPEGLGIV
                     CVPEREDPRDALIAREGHSLATLPIGARVGTSSLRRQAQLLHARPDLEIAMLRGNVDT
                     RLRKVREGQYDAIILAASGLKRLGWDQEVTEYLSVDVSLPAIGQGSLGLEGRTDDSWI
                     RDLVAQFEHRPTRIAVTAERALLAGLEGGCQVPIAGYATLLGDTLTLDGLVTSLDGKQ
                     YIRQVVSGPAEEAESIGMRVAEQLLDGGAQPILQEIYGMA"
     gene            complement(4381..5739)
                     /gene="hemA"
                     /locus_tag="VLA60_00270"
     CDS             complement(4381..5739)
                     /gene="hemA"
                     /locus_tag="VLA60_00270"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HSF07812.1"
                     /translation="MNIIVLGLNHRTASVELRELLAIQDSRMGEALGRLMAYSGIKEA
                     MYLGTCNRVEVYAVVEKGEWGFRQLEDFLVSTHFSLSSEDLLPHLYRYSDDEAIAHLF
                     RVSASLDSMVVGEPQVLGQVKEAYELALTHRSSGVILNKVVKKAISVGKSVRTNTRIG
                     EYAVSVSYAAVELAKKVFSNLKQRVVLLVGAGEMGKLAAQHLVNQGVKEVLITNRNHH
                     RAFTLAERFHGQAVPYEQLRSTLPKVDIVICATGAPHYVITKDDIEMAVYHRMNRPMF
                     LIDITVPRNIDPAVKDVDNAYVFDIDDLKAHVGHNQEERLKEAETAEHMVREEVGSMV
                     AWVRGLEATPTIVALRKKAEEIKQGELEKVFGRLGELSPKERAAIEGLASAIVNKLLH
                     GPVVTLKSEAQSQNGFAFIEAARRFFELREREIHSGEIPLLEDETGDGVSEEPLPQKD
                     GV"
     gene            complement(5736..6545)
                     /gene="ccsA"
                     /locus_tag="VLA60_00275"
     CDS             complement(5736..6545)
                     /gene="ccsA"
                     /locus_tag="VLA60_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSF07813.1"
                     /translation="MSELLPYLTMGLYFGGTLLFLLYLWHRSDAISKISLVATGLGFV
                     FHTSALIMAMVSTGHVPIMTFKDAMSFFAWGLVLVFLVVGLKRGLEVLGAFILPLAFL
                     SLVSANLAPQSADTLAPVFQTVWVHVTLSILGTIGFAVAFVAGLMYVMQERLLKSKQF
                     NVLAFKLPPLDFLDSLNQRSILFGFPLLTLGILTGAVSAQLTIGSYLSWNSEQIWALI
                     TWFFYFAVLMGRVTAGWRAKKAAYLTIVGFAGVILTFVGILIKSPQPMTPL"
     gene            complement(6681..7475)
                     /locus_tag="VLA60_00280"
     CDS             complement(6681..7475)
                     /locus_tag="VLA60_00280"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF07814.1"
                     /translation="MGDIHSLQGQVALVTGGGVGIGRAIAQALVGQQAGVAICGRRVE
                     ELERTAGDLIQAGGEVLWVPCDVTQRIDVERTMKRVIQKFGRLDILVNNAGVSGQTPI
                     HDPDDSKWQNILQVNLTGSYLVSKLAIPHMLTHHYGRIINISSVLGRFGVAGYLAYCT
                     AKHGILGFTKVLALEVASQGITVNAICPTWVDTAMARQGIEETAASLGVSPDEFREIA
                     ISTIPIKRMAEAEEVASVVLYLCSHAASAITGQALNVCGGTTAGTV"
     gene            7601..>8058
                     /gene="rsmI"
                     /locus_tag="VLA60_00285"
     CDS             7601..>8058
                     /gene="rsmI"
                     /locus_tag="VLA60_00285"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HSF07815.1"
                     /translation="MTGTLYIVSTPIGNLEDMTFRAIRILQEVAIIAAEDTRRTQKLC
                     TYYHIGTPLTSYHDFNKEEKTDILLHRLQEGSSVALVCDAGTPLISDPGFYLVRQAIK
                     SNIPLIPIPGPSSVLTALCVSGLATDRFIFEGFVPRKPGARGKFFQNLKE"
BASE COUNT         2122 a         2364 c         1755 g         1817 t
ORIGIN      
        1 aatgagatcc ttcactcctc cacttcgtcc cttaccgaat acaaaatttt ctccaacaaa
       61 cagctttcgg atcccaagcc cgtcatggag aacctgacga acaaactcat ccggcctcag
      121 accggctaac tccctggtga aagggagaat caccagctcc ccgactccta gacgttcaaa
      181 gaacgcaatc ttttcatggt catcagataa aaatttatgg acagatccgg gacgcaacac
      241 ctccacagga tgcggagcaa aggacaacac cataggatat ccattgatct gccgggcaca
      301 atcaacgaca gcctgtacaa gagcacggtg tcccaaatgc tggccatcaa aattgccaat
      361 ggtgagaaca ggacaaggag cgggacgaac cgtcggtaat cctcgagaaa ttttcataca
      421 ccggcacggg tgaggagaca tgcagcctct ttggcaaaat acgttaggat cagatccgcc
      481 cctgcgcgtt taatcgcgag taaactttcc atcatcaccg aagcttcatt gacccagcct
      541 aactgcgcac cggccttgat catgctgtat tccccactta cttgatacgc cgccacggga
      601 acggccacaa gttcgcgaac tcgtcgcaga atatccaaat aaaacaaggc tggcttcacc
      661 atcacgatat cggccccctc ctgaatgtcc agctccacct cacgcaaggc ttccctcgca
      721 ttggctgcat ccatttggta ggaccgtcgg tccccaaaag atgggctcga aaatgccgca
      781 tcacgaaacg gggcatacaa cgccgaagca tacttggcag aataggccat gatgggcgtt
      841 tcagaaaatc cctgttggtc caatgcctgt cgaatcgcgg caacccggcc atccatcata
      901 tcggaaggag caaccatatc aactcccgct tccacatgac tcagcgccat tgcctgaagg
      961 cactctagcg tttcatcatt gagaatccgg ccgtctcgaa caattccgca atgaccatgc
     1021 gaagtatatt catcaataca cacatcggtg atcaccatta aatcgggcaa atggtcttta
     1081 agcgcccgaa ctgcccgctg aacaatccca tccctctcat aggcggaaga tccacgctca
     1141 tctttgaatt caggaatccc aaataagatg acggccggaa cccctgattc ccaggcatct
     1201 ttggcttcct gaacgacccg atccacagac caccgccatt gaccaggcat cgaggcgatc
     1261 ggttcttgct tattctcccc gaaggtcaca aacaagggat agatgaaatc acccggattc
     1321 aggtgcgtct ccctgaccat gcggcgaagg gattcatgtt gccgcaaacg acgtaaacga
     1381 tgaacaggaa atcccatact cgtccttact tccgaggaag atttgttaaa aagaccacta
     1441 gatcccggac cgagatcatc cccaccatgg tgccagcctg ggtaactccc aggtgccgaa
     1501 tatgttcctt agccattaaa tcatttgcat ccaacagggt tcgatcggcc tcgatactca
     1561 caatcggagc ggacataatt ttttcgaccg tcgtccgcat cgggtccgca cccgcggcga
     1621 ccacccgtcg aaccaaatcc gtatcagtta aaatgccgat aatttcctct ccactggtca
     1681 caatcacact cccgattcca ttatcccgca tcatttctgc agcggtcttg actaaaatat
     1741 cccggtcaac tttgacaatt ttgtctaccg ggaccataca agattttaca ggaaccatga
     1801 cgctactcct cccatcaatc ggttaacaaa acgtggtgcc ttgaacttcc tccaccatag
     1861 aatatttatg aagacgatac caatcgcgta tccttcgggt tttgctgggc gtgaatgatt
     1921 atggcatcga ccaacgccgg aaccgtattc tccaacgcca taacatccac cgacagccct
     1981 tcttcctgaa cggtttgcgc ggtgatcggg ccaatgacgg ccacgatcac atgccgtgtt
     2041 atttcctgca attccttgcg atcctcaaac aactggcaaa aatttttcac tgttgaagaa
     2101 ctggtaaagg tcacatactg aatttccctg ttgcgcagtc gatcacgaag agggcccata
     2161 tcgacgatag gaggaagagc ttgatacgca tgaaccacat gaaccgtggc ccccatggct
     2221 ttcaactggt caggaagaat ctcgcgtgcc actttggctc gaggaatcaa aatccgttgc
     2281 ctggcgaccc ctaacccgcc gagggcctgc aaaatcccct ctgcctgaaa atcttgagga
     2341 accaaatccg aggcaatccc ccaacgagca gcctcctccc gtgtccgagg accaatacaa
     2401 cacacacgga ttccggccaa gctccgcaca tctttgtgat gaaaccagag acgttccatg
     2461 aaggattgca cgccattcac actcgtgaaa atcacccagt cgtatgtcga aatcgccgca
     2521 atcgcctcat ccacaggtgt ccagctatca ggaggatgaa tctccaacgt aggacattcc
     2581 accggttccg caccctgcgc cgccagcaaa tttgaaagag ccggcgcctg ggcacgaggt
     2641 ctggtcacta aaaccccttg tccaaacaga ggccgtgatt catgccagtt cagtcgttca
     2701 cgtaaccgaa cgacttcacc cacgacaatc acagagggcg gactcatgtc tgcctgagag
     2761 gctaatccca caatgtcgga taacgttccc accactgttt gttgacgggc ataggtcccc
     2821 catcgaatca ccgccacggg agtcgatgca ggcttgcctt cctgaatgag ccggtccgca
     2881 atctgcggaa gatttttcat acccatcaag aagactaggg tcccttcagc cgaagcgaga
     2941 cgcggccatt ccatcgcact cgatggtttg gtcggatctt catggccggt cacaaatgcg
     3001 accgtcgagg cgagagtccg gtgtgtcacg ggaatgccgg cataagccgg cacagccaca
     3061 gcagccgtca ctccaggtac gacttcgaat gggatcccag cgtcagcgat tacttcggct
     3121 tcctcacctc cacggccaaa cacaaaagga tccccgccct tgagtcgcac cacacatttg
     3181 ccttcttgcg ctttggtaac caacaaggca tggatctctg cctgatcgcg ataggcacca
     3241 cgcccttttc tgccgacgta aatacactcg gcgtgagaag gggcaaattt caacaactct
     3301 ggattagcca aatgatcgta gagcaccacg tcagcctgct cgagacattc tttccctcgt
     3361 aaggtcagca acttggcatc tccgggaccg gctccaacca aaaacactcg accatgattt
     3421 gcaactgact tggtcatgcc atcccataaa tttcctggag aatgggctgc gccccaccgt
     3481 ccaacaattg ttcagccacc ctcatgccaa ttgattcggc ttcctccgca gggcccgata
     3541 ccacttgacg aatatattgt ttcccatcaa ggcttgtgac taacccatcc agagtcaggg
     3601 tgtcccctag gagagtcgca tacccggcaa tggggacctg gcatccgcct tccaaccctg
     3661 caagcaacgc ccgttcggca gtgaccgcaa tccgagttgg ccgatgttca aactgtgcga
     3721 ccagatctcg aatccacgaa tcatctgtcc gtccttccag acccaacgaa ccttgcccaa
     3781 ttgccggcag actcacatcc acggacaaat actccgtcac ttcctgatcc catcctaagc
     3841 gcttgagtcc ggaggcggcc agaataatgg catcatactg accctcgcga accttgcgaa
     3901 gtcgtgtatc aacattccca cgcaacatcg caatctcaag atccggacga gcatgtaaca
     3961 actgcgcctg tcgtcgtaaa ctactggttc cgactcgtgc accgatcggc aatgtcgcca
     4021 agctatggcc ttcacgggca atcaacgcat ccctgggatc ctcccgctcg ggaacacaaa
     4081 caatccccag tccttcaggc aattctgacg ggacgtcttt catgctatgc acggccagat
     4141 caatatctcg tcggagcagg gcttcttcga tttctttgac aaacaggcct tttcctccaa
     4201 ttttggccaa aggcacatct tgaatcttat ctccgctggt ttgaatgcgc ttcagaatga
     4261 cagaaatatc cggagcaagg gctttgagct gactttgaac ccattcggcc tgccatatag
     4321 ccaattggct tcctcgtgtt cccacaatga gcgtagacct tgttcgactc ttcccctgat
     4381 tcatacgcca tccttctgcg gcaggggctc ctcgctgacg ccatcacctg tctcatcttc
     4441 caggagtggg atctctccgg agtgaatctc ccgctcacga agttcaaaaa accggcgggc
     4501 ggcttcaata aacgcaaatc cattttgaga ttgcgcttcc gactttaagg tcacgaccgg
     4561 gccatggagt aatttattca caatcgccga ggccagccct tcgatagcag ctcgctcctt
     4621 cggcgacaat tctcccagac gaccaaagac tttctccaac tcgccttgtt tgatttcttc
     4681 cgcttttttt ctcaaggcta caatagtggg cgttgcttcc aggccacgaa cccaggccac
     4741 catacttccg acttcttccc tcaccatgtg ctcggcggtt tcggcttctt tcaaccgttc
     4801 ttcctgatta tgccccacat gggctttgag atcatctatg tcaaatacat aggcgttatc
     4861 aacatccttg accgccggat caatattgcg aggcaccgtg atatcaatca aaaacatggg
     4921 acggttcatt cgatgataca ccgccatttc gatatcatcc ttggtaatga catagtgggg
     4981 agcacctgtg gcgcaaatca caatatccac cttaggtagc gtgcttcgaa gctgttcata
     5041 gggaaccgcc tgcccatgaa agcgctcggc cagtgtaaaa gcacgatgat gattccgatt
     5101 ggtaatgagg acctccttca caccctgatt caccaggtgt tgcgccgcca actttcccat
     5161 ctccccggca ccgactaaca acacgactcg ttgcttcaga ttcgaaaaaa cttttttggc
     5221 taattccaca gcggcatagc tgacggaaac cgcatattcc ccaatccgcg tatttgtccg
     5281 gacacttttt cctaccgaaa tcgctttttt gacgacctta ttaagaatga ctccggacga
     5341 acgatgggtc agagccagct cataggcttc cttgacctgc cccaggacct ggggctctcc
     5401 cacgaccatg gaatctaaac tcgccgatac ccgaaataaa tgcgcgatgg cttcatcatc
     5461 actatatcga tacaggtgag ggagtaaatc ctccgaagac aaggagaaat gggttgagac
     5521 caaaaaatct tccaattgac gaaaacccca ttctcccttt tccacgacgg catatacttc
     5581 aacacggttg caggtcccca gatacatggc ctccttaatt ccggaatacg ccatgagccg
     5641 acccaaggct tctcccatcc ggctatcctg gatggccagt aattcacgaa gttcaacgga
     5701 agccgtccga tgattaaggc ctaaaacgat aatattcata acggtgtcat cggttgagga
     5761 cttttgataa ggatgccgac aaaagtcaaa attactccgg caaatcccac aatggttaga
     5821 taagcggctt tttttgccct ccagccggcc gtcacccttc ccatcaggac cgcgaaataa
     5881 aagaaccagg taattaacgc ccaaatttgc tcggagttcc aactcaaata cgatccaatc
     5941 gttaattggg ctgaaacggc ccctgtcaga attcctaggg tcaataaggg aaacccaaaa
     6001 aggatagatc gttgattgag agaatctaaa aagtccagcg gtgggagctt gaaagctaac
     6061 acattaaact gtttggactt caagagacgc tcctgcatca catacatcag cccggccaca
     6121 aaggccacag caaatccgat ggtacccagg atactcagtg tcacatgcac ccataccgtc
     6181 tggaagaccg gggcaagggt gtcagctgac tgaggggcca ggtttgccga gacgagagac
     6241 agaaaggcca atggaagaat aaaggcgccc aacacttcta agccccgttt caggccgacc
     6301 accaaaaaga ccagcactaa cccccaggca aaaaaagaca tcgcatcctt aaatgtcatg
     6361 atagggacat gccccgtcga taccatagcc atgatgagtg ccgaggtatg aaaaacaaac
     6421 cccaaacctg tggccacaag ggaaattttt gagatggcat cagaacggtg ccagagatac
     6481 aaaagaaaaa ggagcgtgcc tccaaaataa aggcccatgg tgagatatgg cagtaattca
     6541 gacattgaac aacctagaaa tgaaacatgc gcagtgagcc atgtcgagta tagatctgca
     6601 tctacagaag gtcaagaaaa tcaatgagtt accagcattt cttggttaac cggaatagga
     6661 aaattacatc gcgtatccgc ttatacagtg cctgcggtag ttccaccaca aacatttaag
     6721 gcttgaccag ttatcgcact agccgcatgt gagcaaagat acaggacaac actggccact
     6781 tcctcggctt ccgccattct ttttattgga atcgtactaa tcgcgatttc acgaaattca
     6841 tctggtgaaa ccccaaggct ggctgcagtt tcttcgatcc cctgtctggc catggcagta
     6901 tccacccacg ttgggcaaat ggcgttcacg gttatgccct gagaggccac ttccagtgcc
     6961 agaactttgg tgaaccctaa aatgccatgt ttggctgtac aataggccaa atatcctgcc
     7021 acgccaaatc gtcccaagac ggaggagata ttaataatac gtccatagtg gtgggtgagc
     7081 atgtgtggaa tagcgagctt cgaaaccaag tagctgcctg ttaaattcac ctgaagaata
     7141 ttctgccatt tggaatcatc cggatcatgg atcggcgttt gacctgatac acctgcgtta
     7201 ttaacaagaa tgtccaacct cccaaacttc tggatcaccc tcttcatggt acgctcaaca
     7261 tcgatccgtt gggtcacatc gcatggtacc caaagcactt ctccaccggc ctgaatgagg
     7321 tcgcctgctg tgcgctctaa ttcttccaca cgccttccac aaatagcgac tcccgcctgt
     7381 tgccccacta acgcctgggc aatggcacgt ccaatgccca ctccacctcc cgtaaccaga
     7441 gccacctgac cttgcaaact atggatgtca cccatccttc atatccttat catttggcaa
     7501 ctgagtgacg aggcgggtac gactggatcc tcctggacaa tgtcaggctc acgcagtacc
     7561 atctccaacc ttgccctata tcattttccc tcattagacc atgaccggaa cactctatat
     7621 tgtcagcaca cctatcggaa atctggaaga tatgaccttc cgggccatcc gcattctaca
     7681 agaggtagcc attattgcag ctgaggacac acgtcgcaca caaaaactct gcacctatta
     7741 tcacatcggc acaccactca ccagttatca cgatttcaat aaagaggaga aaaccgacat
     7801 tctcctccat cgtctccagg aaggaagttc cgttgccttg gtttgcgatg cggggacccc
     7861 tcttatttct gacccggggt tttatctggt tcgccaggcg atcaagtcaa atattccact
     7921 tatccctatt ccaggaccat cctccgttct gacggcctta tgcgtgtccg gtttggcaac
     7981 ggatagattt atctttgagg gatttgtgcc cagaaaacca ggggccaggg gaaaattttt
     8041 ccaaaacctt aaagaaga
//
LOCUS       DATIAL010000009        14867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_754803, whole genome shotgun sequence.
ACCESSION   DATIAL010000009 DATIAL010000000
VERSION     DATIAL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14867
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754803"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..461
                     /locus_tag="VLA60_00290"
     CDS             126..461
                     /locus_tag="VLA60_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07816.1"
                     /translation="MTKLPKTIEDLPFSAQAVSFAEIIKNGEIPKQYLDSEYVMHQFV
                     ERLVHYILSVPQGKFSMSELGKLLEKMDPTHQVFFFKRLKENSPNSLKQFAPLYYGFM
                     AEFHPLLFT"
     gene            587..955
                     /locus_tag="VLA60_00295"
     CDS             587..955
                     /locus_tag="VLA60_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07817.1"
                     /translation="MNSILHRAVASFYNLIYEGQAFASTMARIDAAEQERYFGRVEGL
                     NWVLDRCQELEDVDMNLTTASLQKLLGDVKSDLEHELSIQRREKGRRADGREEALIFV
                     TDYLVSLITATEIESTKSPV"
     gene            complement(1325..1840)
                     /locus_tag="VLA60_00300"
     CDS             complement(1325..1840)
                     /locus_tag="VLA60_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07818.1"
                     /translation="MVRKGVHKIGGGLKCKQSSKNPLAPQHQGHNDLCAHKSQFSENF
                     LLQRKLSATLRGGKSWSFLARIERSQMVRPEDTSKEKGEIAADLLAILCCPETKQDVC
                     LLDQSVVQRLNQRISKGELKTKGGQLVTEQIDGGLLRKDNSVVYPIRDQIPIMLIEEG
                     ILIEKSDLSST"
     gene            1937..2458
                     /locus_tag="VLA60_00305"
     CDS             1937..2458
                     /locus_tag="VLA60_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSF07819.1"
                     /translation="MTVDIVWALYGVRVPGSGITFFCLLVLWVGMGTTHLLGQQIHRK
                     FEDSLERIPFVRSIYYTLKSMADVLKFRDRFGQSKVVAFPFPRDGLWALGFDMGLPPP
                     RLQVAPEGPLMMVFVPTAIHPFTGYLAFVPEESVNRIQLRPEEAMKMEFSAGLYRPRP
                     GWLSPANVSTKDS"
     gene            2505..2972
                     /locus_tag="VLA60_00310"
     CDS             2505..2972
                     /locus_tag="VLA60_00310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF07820.1"
                     /translation="MSDLNIIVSFNMALARETENRALDVRILESGVEALLKDSGKGWY
                     AVAEKSLDGDRRIVVGQILVTFEWSDWRNGNFWWLQSVYVHPDQRQQQILRQLYEYVQ
                     SQAHLNQEPVCGYRLYVEEKNHQAHKAYAHIGFQETAYKMYEKEFSWTSPSFP"
     gene            3039..4325
                     /locus_tag="VLA60_00315"
     CDS             3039..4325
                     /locus_tag="VLA60_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HSF07821.1"
                     /translation="MRFPGLKQGVQACRISMLGIGWAWTASAVLANPSATQDLVETRP
                     HVKDLGITIGRYASGEMNAITDVQGVKVGHVTIHEGEGALQVGQGPIRTGVTVIIPRE
                     DVWHHKVPAGAFVLNGTGEMTGLAWVAESGFLEYPIALTNTLNVPRVADGVMSWMIQR
                     YPDIGITDDTLTPVVAECDDSRLNDSQGRHVSPEDVVHALETATIGPVPEGSVGAGTG
                     MVSYQFKGGIGTASRVLPSEEGGYRVGVLVNANHGRRHELTIAGVPIGQVYNTDLPTL
                     SHRAHEESTVTALGELPGEVPQRRQDSGSIIIIIATDAPLDARQLSRLSRRATIGLAR
                     TGSISHHGSGDFVLAFSTGNIIPHYPEHRTFFMAHLADTHINPLFQATVEATEEAILN
                     ALLQATTVTGRDGRRFEAISVERLRTILNTYGRPIQ"
     gene            4456..5646
                     /locus_tag="VLA60_00320"
     CDS             4456..5646
                     /locus_tag="VLA60_00320"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HSF07822.1"
                     /translation="MMQCHVALSQEAHPEVAAQAVAASVYEALGQAECHLAFVFFSPH
                     YSSDIQRMVEIIHENLRPQALVGCMGQGVIGGDQEIEEHPGLVLWGLRSTDMRVVPFK
                     LTPNSEGEEASLDGWPVALEATTHPYTFFLFADPFSTPIDELLSLLEEHAPGSSAIGG
                     IASGGMDVGESRLVFNRDIIGSGVVGVAVKGGLEIRTVVSQGCQPIGERYVVTKVDRN
                     VIQELGGIPPLERLETTLKALDEHEQQQAAHGLQVGIAMDEHRPEFGQGDFLIRGLLG
                     ADRESGSLAIADFVQEGQTIQFHVRDAHAASEDFHLLLSKERVTHPDSLPKGALLFSC
                     NGRGRRFFETPHHDVSTLQQQMGTMPIAGFFAGGEIGPVSGKNFLHGYTASIALFYED
                     VSRP"
     gene            5697..6158
                     /locus_tag="VLA60_00325"
     CDS             5697..6158
                     /locus_tag="VLA60_00325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSF07823.1"
                     /translation="MKNVMTLQSGLRLPVFIIGSWIILLIIFVLNPLSGKAAEEAMVT
                     KPSGLQYVDLAIGSGREAHAGETAIVHYTGTLTDGKKFDSSKDRNSPFSFRLGAGRVI
                     KGWDEGVEGMKIGGIRKLVIPPQLGYGSRGAGAAVPPNATLIFEVELLDLR"
     gene            6158..7255
                     /gene="gcvT"
                     /locus_tag="VLA60_00330"
     CDS             6158..7255
                     /gene="gcvT"
                     /locus_tag="VLA60_00330"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HSF07824.1"
                     /translation="MNETPLVSAHRALNAKLVDFAGWLMPIQYTGVLEEYFAVRKAAG
                     FFDVSHMGRIEVSGDQAEAFLQWVSTNDVSRLQKGQAQYGMVCQSSGGILDDVFVYRT
                     GSSSFLICVNASNREKILRWFLMHQSVKFPRAIIRDQSTELAQIAIQGPASKSIMQNL
                     VGVQIETLKPRNCLALEGTEFSGLLSRTGYTGELGYEWYVPAAQVVSAWDRLLKAGAE
                     FEAKPAGLGARDLLRLDVGYLLYGNDMDENTSPLEASAEWVVAWNKGEFEGHLALRKQ
                     QKDGLTRQLAAFEMVERGIPRHDMTIFSKGQLVGKVTSGNFSPILQKGIGLGFVPPAL
                     CKEGMSLEIDIRGKRVQAKIVTLPFYKRPKP"
     gene            7298..7837
                     /locus_tag="VLA60_00335"
     CDS             7298..7837
                     /locus_tag="VLA60_00335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSF07825.1"
                     /translation="MFTEHIYAGKVLILNRETVKLPNGQSTQLEVIRHPGASAVVPIK
                     DDGTVILIRQFRHAAGGFIYEIPAGKLHPQEDPLICAARELEEEIGYRAGQLELLTSI
                     WTAPGFTDEVIHIYRGTDLKEGRQNLDHDEVLEVVEWPLEKALAGIQDGTIRDAKTII
                     GLQLVFLASHSSSAQPVID"
     gene            complement(8048..8881)
                     /locus_tag="VLA60_00340"
     CDS             complement(8048..8881)
                     /locus_tag="VLA60_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HSF07826.1"
                     /translation="MTHSTLLFGGTSILGFNLARLFPETILPFISPGNSSKSVCEWPV
                     LQLENPDWVERTLAQYQPRVLLYCHAVCDVPKCEADPDWAHEINVQHLGRVVEKLPAH
                     IRLVYVSSDHVFGGDGAYHEHSHPCPISVYGQTRVNAEKLVSNRQGSLVIRTGLAIGP
                     SPNGRTGHLDWLRYRTQQHLPITIVEDESRSVVWVTDLARRVMKFAQSTETGIRHISA
                     TRAVSRVELANHLMSILGEPPTFRCESRHQRPAPHLGRVELTSAYADSLSQPLASVLD
                     G"
     gene            complement(8947..9516)
                     /locus_tag="VLA60_00345"
     CDS             complement(8947..9516)
                     /locus_tag="VLA60_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSF07827.1"
                     /translation="MDKVSDYIFVTNVIPQHICQEVLEEIKNKEWKPHTWYNYSADNF
                     KSEPEKELDTLNTTQALQDKLAPFICQSVEEYMMKFAKQEDERIKSFIQTFTPIRFNR
                     YRTGTMMRKHYDHIHSIFDGKYKGIPILSLLGVLNEGYEGGEFLFSSNHEVKLKTGDM
                     LVFPSNFMYPHEVKEVTKGERYTFVGWAF"
     gene            complement(9980..10630)
                     /locus_tag="VLA60_00350"
     CDS             complement(9980..10630)
                     /locus_tag="VLA60_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HSF07828.1"
                     /translation="MKANFSQFHPDQFSFAEDPVLILEDFWSQEERKVVREAMARSKW
                     IALADMPPVAQAFPNCGNWQKSDIGPSEATHFIQRVGMSCIAAYVESFPNIKKRHVNF
                     NYYSYGAGDCLPTHDDTDDLYTYAEGRRPPTRRLALVTYFHEEWHPDWGGELILYGPP
                     TNSKKNTSLQVTHCIPPLPGSLAIFAVPRQHRVCRVDVLAGDHKRLSIAGWFMTEH"
     gene            11125..11577
                     /locus_tag="VLA60_00355"
     CDS             11125..11577
                     /locus_tag="VLA60_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF07829.1"
                     /translation="MNLETIGEVAGQVTIPGNYVDIPWAYHMKKPILLIDDDRPYRQA
                     LRLYLESHGFTAKEAEDGREALVLLDGGLDVDIIISDCHMPVINGLDFLKALSYRVKG
                     QDVRVILVSGNMTKEIAKEATQAGAFALLAKPFDFQELLALVSRGGKP"
     gene            11703..12089
                     /locus_tag="VLA60_00360"
     CDS             11703..12089
                     /locus_tag="VLA60_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF07830.1"
                     /translation="MKAKILLLDDDPELCSTLRDRLVMEGYDVQTASDGRLGLRLYHE
                     SPVDLVITDVLMPEMDGLEVIRELAGKPSPPLMIAMSGGGNRDLGFLVEAAEFGATRT
                     LPKPFLLNDLVSLVKELLSTLPHSST"
     gene            12544..12951
                     /locus_tag="VLA60_00365"
     CDS             12544..12951
                     /locus_tag="VLA60_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF07831.1"
                     /translation="MTTRVVTIEMDDSLEVVRDIFKKVRFHHLLVVDNDRLVGIISDR
                     DMLKAVSPFVGTMSETTRDQATLNKRAHQIMSHHPVTVRSSCSLQEAAELMLARGVSC
                     LPITTIGGEVLGIVTWKDVLRTILDSHGFTGDS"
     gene            13146..14072
                     /locus_tag="VLA60_00370"
     CDS             13146..14072
                     /locus_tag="VLA60_00370"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HSF07832.1"
                     /translation="MVDKSFILELGKLLIGAAWADGKLSSTEVNGLKELLFQLPDISG
                     EEWMELELYMVSPVSEEERQRLLYRVLGRMGSAEDKALALATLGKLLSSEPSGGDQQT
                     EVVQQLKVDLEEGSSGFLEHLRRPFRQILNLRGQRYTDEQDRESRLEDFIKNTIYFQV
                     TMELRDRGITFDLPDAEIRKLCLAAGLMARVAGVDHVVDTVETTAMSAVLKRHWALTD
                     EQAQLVAEISHHRIFRGLDSVRLVKRFKELTTRTERQEFLHCLFEVANAAEQTSFEEI
                     EEIRSIAKGMDLSHQDFLDAKLTIPREDRKGL"
BASE COUNT         3764 a         3331 c         3894 g         3878 t
ORIGIN      
        1 aattgtggta aaaaaattgg tataaatgtt ttgctataca gggaacgcct gatacaatta
       61 cctattaact atttgaaaat gttgatgtct taggcgatga aaccaagttg gagttataaa
      121 gaagcgtgac gaaacttcca aaaaccatag aagatctccc gttttcggcc caagctgtat
      181 cctttgccga aatcattaaa aatggagaaa ttccaaaaca atatttggac agtgaatatg
      241 ttatgcatca attcgtggaa cgtttggttc actatatctt aagtgttccg caaggcaaat
      301 tctcaatgtc tgagttagga aaactgttgg agaaaatgga cccaactcac caggttttct
      361 tttttaaacg gctcaaagaa aatagcccca atagtttgaa gcaatttgct ccattatatt
      421 atggatttat ggcggaattt catcccttat tatttaccta agggcaccac accaataaaa
      481 aggtcacttc gttttacaat taaaataaaa aagtcctaga aggatcctga ttaggagtat
      541 ataatcgaag agagagtaca ataatttttt ttgtgaggaa cggataatga attcaatttt
      601 acatcgtgcc gtggcgagtt tttacaacct catttacgaa gggcaggcat ttgcctcaac
      661 catggcccga attgatgcag ctgagcaaga aaggtatttt ggtcgggtag aagggctcaa
      721 ctgggtttta gatcgctgtc aagaattaga ggatgtggat atgaacctga cgacagcctc
      781 cctccagaag ttattagggg acgtcaagtc ggatctggaa catgaactct ccatacaacg
      841 tagggaaaaa gggcgtcgtg cggatgggag agaagaagcc ctaatttttg taacggatta
      901 tctcgtcagt ttgatcactg ccacggaaat tgaatcaacg aaaagccctg tgtaattgcc
      961 tgccaatcag tgttggacca ttccaggaat ggtccaagct ctgctctttc tactgagtac
     1021 tcggaactaa aatggggttg tctcgaaaaa caatggtccg tgagtggatt atcttagccc
     1081 tatgtttagg gttgggggcc catgtggcat tgggatttct tctgcatggc ggacagaatt
     1141 ggccgattga atcgtatgga tattatgggg gtttttttgg agtctttatc tatgtggtag
     1201 tccagatttg tcgatcacta tggtggctta aaaaaggtgg taggagtaca caaggattat
     1261 aaaaaggcta ttccaaaaaa aaataattac cgattttttc ttaaaaaaca attgtaagag
     1321 agccttatgt tgatgaaagg tcggatttct ctatgagaat gccttcttcg ataagcataa
     1381 tgggaatttg atcacggatc gggtagacca cactattgtc ttttcgaagg agtcctccat
     1441 cgatctgttc agtgacgagt tgacctccct tcgtcttcaa ttctcctttt gaaattcttt
     1501 ggttcagtcg ttgaacaacg ctttggtcca ataaacacac atcttgcttt gtctcgggac
     1561 aacagagtat tgccaatagg tcagcagcaa tctcaccttt ttctttagat gtatcttcag
     1621 ggcgcaccat ctgactcctt tcaatacgtg ccaaaaatga ccaactcttc ccaccacgaa
     1681 gagtagcact gagcttcctt tgtagcaaga aattttcaga aaattgggat ttgtgagcac
     1741 acaaatcatt gtgaccctga tgctgcggag cgagtgggtt tttgctagac tgcttgcatt
     1801 tcaatcctcc gcctatttta tgcacgccct ttctgaccat actttcaaac ggtacctcct
     1861 gactggttta tttcttatta ttccagcctg ggggacctta ttaatcctct acacgctact
     1921 cgaagcatta gagcatatga cagtagacat tgtgtgggcg ttatatggcg tgagggttcc
     1981 cggttcaggg attacctttt tttgtcttct tgtcctctgg gtgggcatgg gaacgacgca
     2041 tttacttggc cagcaaattc atcgaaaatt tgaagattct ttggagcgaa ttccttttgt
     2101 gcgtagtatc tactataccc ttaaaagtat ggcggatgtc ctcaagttcc gcgatcggtt
     2161 tggtcaaagc aaagttgtcg catttccgtt tcctcgtgac gggctttggg ctttagggtt
     2221 tgacatggga ttacctccac ctaggctaca agttgcccct gaaggacctc taatgatggt
     2281 ctttgttccg acagccatcc atccgtttac cggctatctg gcctttgtcc cggaagaaag
     2341 tgtgaatcga attcagttac gaccagaaga ggcaatgaaa atggagtttt cagccggact
     2401 ttaccgcccc cgaccgggat ggttgtcacc agcaaatgtt tcgacgaagg atagttgacc
     2461 tgggaatgga tatgtcatcc ccggtaataa tcaggcgggc aaccatgtct gatctgaata
     2521 ttattgtaag tttcaacatg gctttagcca gggaaactga aaatcgcgca ttagatgtgc
     2581 ggattcttga gtccggtgtc gaggcacttc tcaaagattc tggtaaggga tggtatgcag
     2641 ttgcagaaaa gtctcttgat ggtgaccgac gcatagtagt tgggcagata ctggtcactt
     2701 ttgagtggag cgattggcga aacgggaatt tttggtggct tcaaagtgtg tacgtgcatc
     2761 cagatcagcg tcaacagcaa atcttacggc agctttatga gtatgtgcaa agccaagccc
     2821 atttgaacca ggaacccgtt tgtggatatc gcctctatgt ggaagagaaa aaccatcagg
     2881 cccacaaggc ctatgcccac atcggctttc aagaaaccgc ctacaaaatg tatgagaaag
     2941 aattttcctg gacatccccg tcctttccct aatcaatcgt gtgcatgcat tttccctgct
     3001 aagggtgaat gggctagagg acaggtaaga ctggcaatat gcgttttccc ggattgaagc
     3061 agggcgtaca ggcctgtcgt attagcatgt taggaattgg ctgggcatgg actgcctctg
     3121 cggttttggc caatccctct gccactcagg atttggtaga aacccgcccc catgtgaaag
     3181 atcttgggat caccataggc cggtatgctt cgggggaaat gaatgccatt accgatgtcc
     3241 aaggggtgaa agtgggacat gtcaccattc atgaaggcga gggggccttg caggtaggtc
     3301 aaggcccgat tcggaccggt gtgaccgtta ttatcccgcg tgaagatgtt tggcatcata
     3361 aagttccagc tggagcgttt gtattaaatg ggacagggga aatgacgggt ttggcctggg
     3421 tggcggaatc aggattttta gaatatccaa tcgcgttaac gaatacgttg aatgtccctc
     3481 gtgtggcaga tggcgtcatg agttggatga ttcagcgcta tcccgacatc gggataactg
     3541 atgatacgct cacgcctgtg gtggctgaat gcgacgatag tcgactgaat gatagtcagg
     3601 gaaggcatgt gtctccggaa gatgtggttc acgccctgga aacggcaaca ataggtcctg
     3661 ttccagaggg gtctgtcggg gcagggacgg ggatggtgtc ctatcaattt aaaggtggca
     3721 ttggtacggc ctctcgagtg cttccatccg aagaaggcgg atatcgtgtc ggagtgttgg
     3781 tgaatgctaa ccatggccgt cgtcatgaat taacgatcgc gggagtgccg ataggccaag
     3841 tctataatac ggacctgcct accctttcac acagagccca cgaagaatcg accgtaacag
     3901 ctctgggcga gcttccggga gaggtccctc aaagacggca ggacagcggg tccattataa
     3961 tcattatcgc gacggatgct ccgcttgatg cacgccaact gtctagactc tcgcgccggg
     4021 ctaccatagg cttggcgcga accgggtcta tctcccatca tggcagtgga gattttgtcc
     4081 tggcgttttc caccggcaat attattcccc attatccgga acaccggaca ttttttatgg
     4141 cccatcttgc cgatacccat atcaacccct tgtttcaagc cacggtggag gccacggaag
     4201 aggccattct caacgctctg ctgcaagcca caacggttac agggagagat gggcgacgtt
     4261 ttgaggcgat ctctgttgaa agactccgta cgattttaaa tacctatggt cgcccaattc
     4321 agtaagcaac ttacgtctag ggtgaatgtt ctttggtttt atcgggacgg gttaggaaaa
     4381 gaggacaaaa attcaccaaa tctctttcat ggacatcgtt tggcggggat tgctagactt
     4441 cgaaacaatt gaattatgat gcagtgtcac gttgcccttt cccaagaagc tcatcctgaa
     4501 gtggccgctc aggcggtagc cgcctcagta tatgaggcat taggccaagc ggagtgtcat
     4561 ctcgcatttg tctttttttc tccccattat tcctcagaca ttcagcgcat ggtcgaaatc
     4621 atccacgaga accttcgtcc tcaggcactg gtgggatgta tgggacaagg ggtaattgga
     4681 ggcgatcagg aaattgaaga acaccctggt ctggtactct ggggccttag aagcactgat
     4741 atgcgagtgg tgcccttcaa gttaacgcca aactcagagg gtgaggaagc gtcactagat
     4801 ggctggccgg tggcattaga agccaccacc catccttaca cgtttttcct ctttgccgat
     4861 ccgttttcta cccccataga tgaattattg tccctgttgg aggagcatgc tcccggctcg
     4921 tccgctattg gaggaattgc cagtggaggc atggatgttg gggaaagccg cctggtcttt
     4981 aatcgcgaca ttattggatc cggggtcgta ggcgtggctg ttaagggtgg attggaaatc
     5041 cggacggtgg tgtctcaagg gtgccaacct attggtgaac ggtatgttgt gaccaaggtc
     5101 gatcggaatg tcattcagga gcttgggggg atcccgccgt tggaacggtt ggaaaccacc
     5161 ttgaaagcgt tggatgaaca cgaacaacaa caagcggcgc acggactgca agtgggtatt
     5221 gccatggacg aacatcgacc ggaattcggt caaggggact ttttgatccg tggtctgtta
     5281 ggagccgatc gagaatctgg gagtctcgcc atcgcggatt ttgttcagga aggccagacg
     5341 attcagttcc atgtgcggga tgctcatgcc gcgagtgaag acttccattt acttttatcg
     5401 aaggagcggg tgacccatcc ggacagcctg cctaaaggcg cgttgctatt tagttgtaat
     5461 gggcgtgggc ggcgattttt cgaaactcct caccatgatg tgtccactct gcaacaacaa
     5521 atggggacca tgccgatagc ggggttcttt gcaggtggag aaattggccc cgtgagcggg
     5581 aagaattttt tgcatggcta tacggccagt atagcgctgt tttacgaaga cgtgtctcgc
     5641 ccataaaaaa tgggcatggc atcggatacg tttcacacta gcaaagagag aggttcatga
     5701 aaaacgttat gacattacaa tcaggtcttc gattacccgt attcattata gggtcttgga
     5761 tcatccttct cattatattt gtattgaatc ctctttccgg gaaggctgcg gaagaagcga
     5821 tggtgaccaa gccttctgga ttacaatatg tcgatctcgc cattggttcc gggcgtgaag
     5881 cccatgcggg agaaaccgcc atcgtccatt acacggggac actcaccgac ggaaaaaaat
     5941 tcgatagctc taaagatcgt aattcgcctt tttcctttcg attaggggcg ggccgggtga
     6001 ttaagggttg ggacgagggc gtcgaaggga tgaaaatcgg cggtatccgc aaattggtaa
     6061 tcccgcctca attgggatac ggttcccgag gggccggagc agctgttccc cccaatgcca
     6121 ctctcatctt tgaagtcgaa ctgctcgatc tgcgttgatg aacgaaaccc cactcgtttc
     6181 cgcccatcgt gcgctcaatg ccaagctggt ggatttcgcc ggctggctca tgcccatcca
     6241 atataccggt gtccttgagg agtattttgc ggtcaggaaa gcggctggtt tcttcgacgt
     6301 cagtcatatg ggacgcatcg aggtatcagg agaccaggct gaagcctttc ttcaatgggt
     6361 cagtaccaat gacgtcagtc gcctccagaa agggcaggcc caatatggca tggtctgcca
     6421 atcctccgga ggcatcctcg atgacgtgtt tgtctataga accgggtcct cctcgttttt
     6481 gatttgcgtc aatgcctcca atcgggaaaa gattcttcgg tggttcttga tgcatcagtc
     6541 ggtaaaattt ccgcgagcga tcattcgtga tcaatcaaca gagttggccc aaattgccat
     6601 tcaagggccg gcatcgaaat ccatcatgca aaacctcgtg ggggtgcaaa tcgaaaccct
     6661 gaaaccgcgg aattgtttgg cgctggaggg cacggagttt tccgggttgc tgagtcgaac
     6721 gggatatacc ggagagcttg gatatgagtg gtatgtccct gcggcgcaag tggtctccgc
     6781 gtgggatcga ctactgaagg ccggtgcgga attcgaagcc aagcccgcag ggctaggagc
     6841 gcgagatctt ttgcggttag acgtcggcta cttgctgtat ggcaacgata tggatgagaa
     6901 cacctccccg ctggaagcaa gtgccgagtg ggtagtggcc tggaataaag gcgagtttga
     6961 agggcatctc gcgttacgca agcaacagaa ggatggtctc actcgtcagc ttgctgcctt
     7021 tgaaatggtg gaacgcggta ttccccgtca cgacatgact attttcagca agggacaatt
     7081 ggtgggtaag gtcaccagtg gaaatttttc gccaattttg caaaaaggga ttggcttagg
     7141 atttgtgcca cctgcactgt gcaaggaagg catgtcgctt gaaatcgaca ttcgtggaaa
     7201 acgggtacaa gccaagatcg tcacacttcc gttttacaag cgtccaaaac cttaaaacgc
     7261 cattgtattc atcattctga ctgaaacgag aactcctttg ttcaccgaac atatctatgc
     7321 cggaaaagtc ttaattctca accgcgaaac ggtaaaattg cctaatgggc agtccaccca
     7381 attagaagtc attcgccatc ctggcgcctc ggcggtcgtg ccgatcaaag atgacggtac
     7441 cgttatcctc attcggcaat ttcgccatgc agcgggagga tttatttatg aaatccccgc
     7501 gggcaaacta catccccagg aagacccgct catctgtgca gcacgtgagc tggaggaaga
     7561 gattggctat cgggccggtc agttggagtt gctcaccagt atttggaccg ctccaggatt
     7621 tacggatgaa gtcatccata tctatcgggg aacggatctg aaggaaggac gccagaattt
     7681 ggaccacgat gaagtgttgg aagtggtgga atggccgctt gaaaaggcgt tggccggtat
     7741 tcaggacggc accattcgtg atgccaaaac aatcatcgga ttgcagcttg tatttctagc
     7801 ctcccattct tcatccgccc agccggttat agattgatcg aaagcgtccg gactcctcgt
     7861 aatgggtaca ctcgccacac atttaggaag aaacttccgg aacgcagttg aatcccacag
     7921 ttcaattttt caaaaaccag gtatctgggc gttgttctga ttttttaagc aagcaagacc
     7981 agttggattg tcaggggctc aatctgtgag acaatctaca ggacggcgta tttgagaatt
     8041 agattgttca gccatccagg acgcttgcca agggctgaga aaggctatct gcataggcgc
     8101 tggttaattc cacacgcccc aaatgggggg ccggccgttg gtgacggctc tcgcaacgga
     8161 aagtgggagg ttctcccaaa atcgacatca gatgattggc caattccact ctcgatacag
     8221 cccgagtggc ggagatatgc cggatgcctg tctcagtgct ttgtgcgaat ttcatgacgc
     8281 gtctggccag atccgtcacc cacacaacgg agcgagattc atcttcaaca atagtaatgg
     8341 gaagatgttg ttgagtgcgg taacgaagcc aatccaaatg ccctgtgcgc ccgttcgggg
     8401 aagggccaat cgccagaccc gttcgaatga ccagtgaccc ctgtcgattc gagacaagct
     8461 tttctgcgtt gacacgggtc tggccgtaca cactaatggg gcacgggtgg gagtgttcat
     8521 gatatgcacc atccccgcca aagacatgat ccgaggacac atacaccaat cggatatggg
     8581 ctggtagttt ctccaccaca cgaccgagat gttggacgtt gatctcatgc gcccaatcgg
     8641 ggtcggcttc acacttcggc acatcacaca ccgcatggca atacaacagg accctcggct
     8701 gatactgagc caaggtgcgt tccacccaat ctggattttc caattggagg accggccact
     8761 cacaaactga tttcgaagaa ttgcccgggg agataaacgg cagaatggtt tccggaaata
     8821 atctggcgag gttgaaccca agaatggagg tcccgccgaa gaggagcgtg gaatgggtca
     8881 tccgcaccga gtttttgagg ccataggata aatcatgagt gttgcgatct cccgaagtcg
     8941 cacaatttaa aatgcccaac ccacaaaggt gtatcgctcg cctttggtga cttctttgac
     9001 ttcatgtgga tacataaagt tagaaggaaa gacgagcata tccccggttt taagtttcac
     9061 ctcatgatta gatgaaaaga ggaattcgcc accttcatag ccttcgttca gcacccctaa
     9121 taaggacaag atggggatgc ctttatattt cccgtcaaaa atcgagtgaa tatgatcgta
     9181 atgtttcctc atcatggtcc cggtgcggta ccggttaaac cgtatgggtg tgaaggtttg
     9241 gataaaactt ttaatgcgtt catcttcttg tttcgcaaac ttcatcatat attcttcgac
     9301 ggattggcaa ataaacgggg cgagcttatc ctgtagagct tgggtggtat tgagagtatc
     9361 taattctttt tcaggctcgg atttaaaatt atcggccgaa tagttgtacc aggtatgggg
     9421 tttccactct ttgtttttaa tttcctctag aacttcctgg caaatatgtt gaggaatcac
     9481 gttggtcaca aaaatataat ccgatacctt atccattccc catctcctta ccattcagat
     9541 gtctgacgcc gccagcggaa gcttgaaatg gcaacatctt cactcaattc cggaatcagt
     9601 atttccaatc cctctggccc aatgaaaccg tcatgcgatt gtaatcccac aatggactgt
     9661 gtggccagtc ctcagaatcc catgcttcta tatgtggtgc tcccggcatt actgggggga
     9721 cctttttgaa catcctgctt cgttcaggga ggttcgtaaa atttttgatc tctataagct
     9781 ctcactctca gaataatctt aagtttttca acagctcgct agggtacggc aacatctcac
     9841 ccatctgagg cactttgtga accttcacgg caggaacgtc aagctgggaa ggccctgctg
     9901 catcacagct tttagaagtg ggatctccct ttgagttgaa atgataggac tgaaaaatgt
     9961 ttgggtagtg tgaggtgttt caatgttctg tcatgaacca tccggcgatg gagagacgtt
    10021 tatggtctcc agcaagaacg tccacccggc agacccgatg ttgtcgaggc accgcgaaaa
    10081 tggccaaaga tcccggcaat ggaggaatgc aatgtgtcac ctgtaatgac gtattctttt
    10141 tagagtttgt gggaggacca tacaagataa gttcccctcc ccagtccgga tgccactcct
    10201 cgtggaaata ggtgaccaag gcgagacgac gagttggcgg tcgacggccc tcggcatagg
    10261 tatagaggtc gtcggtatca tcgtgggttg ggagacagtc acccgcgcca taggaataat
    10321 agttaaaatt aacatgccgc ttttttatgt ttgggaagga ttcaacatag gcggcaatgc
    10381 aggacattcc gacccgttgg atgaagtgag tagcctcaga tgggccaatg tctgatttct
    10441 gccaatttcc gcaattggga aaggcttgag ccacgggtgg catatcggcc agggcaatcc
    10501 acttcgatcg tgccattgct tcccgaacaa ccttccgttc ttcctgactc caaaaatctt
    10561 ccaggatcaa caccggatcc tcggcaaacg aaaattggtc gggatgaaat tgagagaaat
    10621 tggccttcat gcttcagggg caatcaaaag ttaggattgg atgcagaaaa cttggagact
    10681 tcttttagca agtttctcat agggtatcaa atgagaaatg ggagacatgt tcggaataaa
    10741 aacttcttta aggttgccct aggtgaaggg cctgtgcccc tgtctcatcg ctagagacgt
    10801 gttttccttt cttgagaaaa ccaaggaggt tttaggaagc atgataaata tttccttagg
    10861 agagcatctt ctaaaggcct tgctctacag gcatcccaag ggatacgccc cttatccatt
    10921 tagataactg aattgggact tccctcactc ctaatgggta acgggaagag cactaatgaa
    10981 attttacttt taatttcaag gtgttacttc aaataattta ttgaaagcta tggcaggaag
    11041 agcttggcaa tacttttgcc tgtataggaa gttgggcgga atagggacca atcggttcat
    11101 atccttttta tcaggtagcg attcatgaac ctggaaacga taggcgaggt cgcaggccaa
    11161 gtgacgatcc ccggtaacta tgtggatatt ccatgggcgt accatatgaa aaaaccaatt
    11221 ctgttaattg acgacgaccg gccatacagg caggccttgc gcctgtattt ggagagtcac
    11281 gggtttactg ctaaggaagc cgaggatggc cgggaggccc tggtcttgtt ggatggaggg
    11341 ctggacgtgg acatcatcat atcagattgt catatgcccg ttatcaacgg gctcgatttt
    11401 ctcaaagccc tgtcctaccg tgtgaaggga caggatgttc gagtcatact tgtgagtggt
    11461 aatatgacaa aggaaatagc aaaggaagcc acacaagcag gggcttttgc gcttctggcg
    11521 aaaccgttcg attttcagga gcttctggcg ttggtgtccc gaggtggcaa gccataatct
    11581 cctcaaaggc gttgtgtgag ggggtgtcat aaaattcttg accatgaaat ccgacaaaac
    11641 cctcaaaaaa tatgcaaaat tgcgtcggga ggaaacggta tcgagaccct ggtgaggtgg
    11701 atatgaaagc gaaaatttta ctcttggatg atgaccccga attatgttca accttgcgag
    11761 atcggttggt gatggaaggc tatgatgttc agactgcctc cgatggccga ctggggctaa
    11821 ggctctatca tgagagtccc gttgatctgg tcataacgga tgtgctcatg ccagaaatgg
    11881 acggcctaga agtgatacgg gaacttgctg ggaaaccttc tcctccatta atgattgcca
    11941 tgtcgggagg tgggaaccgt gatttgggat ttttggtaga ggcggctgag tttggtgcca
    12001 cccgcacact ccccaagccc tttctcctga atgatctggt gagccttgtg aaagaactgc
    12061 tgagtaccct gccccattca tcgacatgaa tcttttgttc cactccgacg ggcatgcttt
    12121 gccaagatcc gtgaatctgc aaaatggtga tctgacgtac ggtggtggga aaaccagaac
    12181 gaggaatggt cccccttcct gaggtgaaca gggcaatcag tttgtgtgcg attgctgaaa
    12241 tatgggtaac aagtgaaata ggtgatcgca gtcctagccc ggaatacttc aattactgct
    12301 ctgtggaatc attcggatag aaaagctttc cacatgatgg acaccccctt gttcattgca
    12361 atgttgatgg aatgaagtaa aaaacccctg taaactttta attctaggat gccgaatagg
    12421 agggttgacg gctatagcag ctgacggcca agttttgtgg aaaggactga tttcagtcac
    12481 cccctgccat gaggacctcg taatttggaa tcagagacga gaaccagggt aatcggcaga
    12541 atcatgacca ccagagttgt gaccattgag atggatgatt cactcgaggt ggtccgggac
    12601 attttcaaga aagtccggtt tcatcatcta ctcgtcgtgg acaatgacag gctagtggga
    12661 attatttccg accgggatat gttgaaagcc gtcagtccgt ttgtcggaac gatgtcggaa
    12721 accacccggg atcaagcgac cctcaataaa cgtgctcatc aaattatgtc gcatcatccg
    12781 gtgacggttc gttcgtcctg ctctttgcag gaagcggccg aattgatgtt agcccgaggg
    12841 gtctcttgcc ttccaatcac gactattggt ggtgaggttc tggggattgt gacctggaaa
    12901 gatgtgttga gaaccattct ggattcccat ggtttcaccg gtgattcata agtatcttcc
    12961 tacagataac ggtgttgggg gatgcccctt gttccttatt tcaccgggtt ctttagcctc
    13021 tggattccct tcggtatact gaccctagca tttcccacag cattatgaat gttggtgaca
    13081 aggcaggcga gcctggtgaa tgacaggtat gccatgtcta gagtccacag aaagggaggg
    13141 taaggatggt cgataaaagc tttattcttg aacttggtaa acttttgatc ggtgccgcat
    13201 gggctgatgg gaaactgtcg tccaccgaag tgaacgggtt aaaagaactc ttgtttcaat
    13261 tgccggatat ttccggagag gaatggatgg aattggagct gtatatggtt tccccggtaa
    13321 gtgaggaaga acggcaacgt cttttatatc gcgtgctagg acgtatggga tcggcagagg
    13381 ataaagcctt ggctttagcg acgctcggaa agcttctgtc ctcagaacca tcaggtgggg
    13441 atcaacagac agaagtggtc caacagctta aggtcgatct tgaagagggg agtagcgggt
    13501 ttttagaaca tcttcgacga ccatttcgtc aaatcctgaa tctgcggggg cagcgttata
    13561 ccgacgagca ggatcgtgag agccgattgg aggatttcat taaaaacacc atctatttcc
    13621 aggtcacgat ggaattacga gatcggggca tcacttttga tctccctgac gccgagattc
    13681 gcaagctgtg tctcgcagca ggattgatgg cacgagtagc cggcgttgat catgtggtgg
    13741 atacagtaga aaccacggcc atgagtgctg tactcaaacg tcactgggca ttaacagacg
    13801 aacaggcaca actggtggcg gaaattagtc atcaccggat ctttcgaggg ttggatagcg
    13861 tacgattggt gaaacgtttt aaagaattaa cgacgaggac tgaacggcaa gagtttctcc
    13921 attgtttatt tgaagtggcc aatgcggctg aacagacctc gtttgaggaa attgaggaaa
    13981 ttcgaagcat tgcgaaaggc atggacctca gtcaccagga ttttcttgat gccaagctga
    14041 ctataccgcg agaggatcgc aaggggctct aggtgcggag tgtttccatc tattgcagat
    14101 gaatggacga ctgcaattac ttgcggcttc ttaaatgctg cgactgtacc tccggaaaaa
    14161 ttgactaaaa tttttcctat actttcattc ccgacggttt ttatcgggaa tccatcttgt
    14221 gtttttataa ctataatgtt caatcaataa ttggtgatgc cgggtttcgc cccggcaggc
    14281 gacctacttt tctttcggga aaagtaggca aaaccagtga cgccccgtcc ggccacatcg
    14341 gagggtcgga cgcaggattg aagagcggac caactcgctc cgctcagaca aggtccgcca
    14401 gataatgaga gcgtccgacc cgagggccga acggaaggcg tcggatatgg gggagaaggg
    14461 atatggttga aggggttcgg aacattattt acgctacgtg cgtcttgact ccaatgtcag
    14521 ctgtacggtt tgatctcgcc acagactaac tttcttttgc ccctcgccca tctgggggag
    14581 agggcagggt gagggggcaa acgatcaatt gtgaatggac gtgggatgga gccgcaagca
    14641 tttcctccca atggctggcc agaatcagga tgtaaatatg ccaatttaat attgaagtta
    14701 catcataagt taatccttaa gtcttgatag actgtttgtt gggattcccg ttctccccct
    14761 gtcttgcaag cgaaggcttt tcgtcgctct tcctcgctaa gccaatgtat tagtgtcacg
    14821 catccttgtt ttatattacc ccccctttca aaggttcgac gcctgcg
//
LOCUS       DATIAL010000010         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_862530, whole genome shotgun sequence.
ACCESSION   DATIAL010000010 DATIAL010000000
VERSION     DATIAL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862530"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VLA60_00375"
     CDS             <1..460
                     /locus_tag="VLA60_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07833.1"
                     /translation="VMVVDEYDELCHHPDPSKSLIPSEALSYLYVKCRHTLWNDAIVS
                     LVRQLGVYPPGSLVRLSNQKIGIVTSVNLSNRLRPVILVYDEYTSPEEPIVLNLAEED
                     ETLAIVHAIRPVDLSPKIRECLNPRRIISYFPSLASTESGVENQRTLASL"
     gene            complement(514..888)
                     /locus_tag="VLA60_00380"
     CDS             complement(514..888)
                     /locus_tag="VLA60_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07834.1"
                     /translation="MRMNSPPSQASIYHVSGLDDRDRGFNRQVHVIPCEGGFQARLRY
                     ELLQVEVDPVPMEEHALQRLIQVLHDRGYRQLRTQRIFIGDQYLGNQEVWVEYADPDS
                     SSNPEKTWWRWLRQIFRLSQPS"
     gene            1145..2866
                     /locus_tag="VLA60_00385"
     CDS             1145..2866
                     /locus_tag="VLA60_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSF07835.1"
                     /translation="MPSPLTFQSPVERPQKNRGQILTLALLLASIVFVIDLFLPLGIA
                     EAVPYVAVVLIALRSPDKNDPLRLAGLCTLLTVVGYSLSPPGTEPWIGVTNRSIALFA
                     IWSTALLAMQVREADAATRDQSSMLTGILANMPAVAFRIDQEGVMRESIGRGLTRFGL
                     KDFTAIGRIPLEPPDQIKSQFKEGTAANSVFYESKGILQGKPWWFLNCLCKLEGDKKG
                     IIGFGIDITDRKRVERRLALHDAVAAILTSAESITEAYPQILKAICDTLEWKVGILWR
                     MDYQRQALGATAIWPSDSPQGQAMSSPTSDWALTSDVGLAGQVWDIRKPIWVKDLADY
                     TKDPRNETALEVGLHAGFGIPVCLAGNTRAVLEFFSPYPEEADETLLQMLSNIGFQIG
                     QFIEREHKERRLATHHSLTNVLAESPGLTQAAPIILEAICDNLGWDLGAIWTVVPEQQ
                     ILRCIKIWHSPRLNLEVFQRASQDMTFARGFGLPGRVWKSGEPLWITDIGSDPNFPRA
                     SAAAQEGLHSGFAFPIKYGPNIIGVIEFFHRDIQKPDKELLSMFTSIGLQIGQFIQRT
                     ANSASNG"
BASE COUNT          735 a          767 c          681 g          778 t
ORIGIN      
        1 tgtcatggtg gtggatgaat atgatgaatt atgccatcat ccggatccct ccaaatctct
       61 cattccgtct gaggccttat catatttgta cgtaaaatgt cgtcataccc tctggaatga
      121 tgccatcgtt tccttggtca gacaattggg ggtttatcct ccagggtcac tcgtgcgttt
      181 gagtaatcaa aaaattggca tcgtcaccag cgtcaatttg tccaatagac tccggcctgt
      241 tatcctcgtt tatgacgaat atacctctcc ggaagagcct attgtcctga atcttgcaga
      301 agaggatgaa acgctggcta ttgtccatgc cattcgccct gttgacctgt ctcccaaaat
      361 tagagaatgc ctcaatccac gtcggatcat cagttacttt ccttctctcg cttctacaga
      421 atcaggagtg gagaatcaac gcaccctcgc ctccttgtaa atacctccgc cagctttcat
      481 aaaaactcac tatgtcatct gataaaatta tcatcaggaa ggttgtgaga gacggaagat
      541 ttgccgaagc cacctccacc atgttttctc gggattggaa gaagaatcag gatcggcata
      601 ttccacccaa acctcctggt tccccaagta ctgatcccca ataaaaatgc gttgtgttcg
      661 taattgtctg taacctctat catgcaacac ttgaatgaga cgctgaagag catgttcttc
      721 catgggaacc gggtcgacct caacttgaag caattcataa cggagcctgg cctgaaatcc
      781 tccctcacag gggatgacat gaacttgacg gttaaaccca cggtcccggt catccagacc
      841 ggaaacatgg tagatactgg cttgagaggg aggggaattc atacgcatct ggttatcatg
      901 catggattcg catgcttcgc ataggagcaa aaaggaagtc aagccgaaga gaaagcccag
      961 gtgcgttttt tgagtctctg ttacagactt ttgtcgatgc aaagaacacc aatttccccg
     1021 attaagtata aatctgccgg ttcgacttgc acgatctggc agacttttcg tatcgtggca
     1081 ctcaaacaat gctatttaag gatcttgttg tctctccatt tttcttattt atgccattta
     1141 accgatgcca agtcctctaa ccttccaaag cccagttgaa cgccctcaaa aaaaccgagg
     1201 gcaaatttta actctggctc tgcttctggc ctccatcgtc ttcgtcatcg accttttcct
     1261 tcctctggga attgccgaag cggttccgta tgtcgccgtc gtactgatcg ccctccgctc
     1321 acctgataaa aatgatccct tgcgtctggc aggcctgtgt acccttttaa cagtcgtggg
     1381 gtatagttta tctccaccgg gaacagagcc ttggatcggt gtcacaaatc gttctatcgc
     1441 cctgtttgcc atctggtcca cggccctatt ggccatgcag gtgagagagg cggacgctgc
     1501 cacgcgcgat cagagttcca tgctgaccgg cattttggca aatatgccgg ccgtggcgtt
     1561 tcgcatcgat caggaagggg tcatgcgtga atccatcggc cgggggttaa ctcgctttgg
     1621 attaaaagat tttaccgcca ttggacgaat tcctcttgaa cctcccgatc aaattaaaag
     1681 tcaattcaaa gaagggaccg ccgccaattc cgtcttttac gaaagcaaag ggattttgca
     1741 ggggaaaccc tggtggtttt tgaattgcct ctgtaagttg gaaggggata agaaaggcat
     1801 tatcggtttt ggcattgata tcacagaccg gaaacgggta gaacggcgct tggccttgca
     1861 cgatgcggtt gcagctatcc tgacctccgc agaaagcatt acagaggcct atccccaaat
     1921 tctcaaagcc atttgcgata cgctggaatg gaaagtggga atcctttggc gaatggacta
     1981 tcaacgacag gctctgggag ctacagccat ttggccgtct gattccccgc agggccaagc
     2041 gatgtcctcg cccacctcgg attgggcgtt gacttctgat gtgggactgg ccgggcaggt
     2101 gtgggacatc cgcaaaccga tctgggtcaa agatcttgct gactacacca aagatcctcg
     2161 aaacgaaacc gctctggaag tcggacttca cgccggtttc ggaatcccgg tttgcctggc
     2221 cgggaatacc cgggcagtac tcgaattttt ctcaccatac cctgaggaag ctgatgaaac
     2281 attgcttcaa atgctttcaa atatcggatt tcaaatcggc cagtttatcg aacgtgaaca
     2341 caaagaacgt cgattggcga cccaccacag cttaaccaac gtgcttgcgg aatcaccggg
     2401 cttaacccaa gccgctccaa ttatcctgga ggctatttgc gacaatttag gatgggatct
     2461 cggagccata tggacggtgg ttcccgagca gcaaattctc cgctgtatca aaatttggca
     2521 ttccccccgc ttaaacctgg aagtctttca gcgggcctca caggacatga cgtttgcccg
     2581 aggattcggt ttaccaggtc gcgtttggaa aagtggcgaa cccctctgga tcaccgatat
     2641 agggtcagac ccgaattttc cacgtgcctc cgcggcggca caggaaggcc tccattccgg
     2701 atttgcattt cccattaaat acggccccaa cattattggt gtcatagaat tctttcatcg
     2761 ggatattcaa aaacctgata aagaactctt gtccatgttt acatccattg gtctccaaat
     2821 cggccaattt atccagagga cagcaaactc tgcttccaat ggataatcgt attcacgtga
     2881 atttttgttc caacctgatt cattcgcaac ctctcacatg atcgtggcat ttctcttcct
     2941 gggaaagtat ttcacaaacc a
//
LOCUS       DATIAL010000011         4039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1507731, whole genome shotgun sequence.
ACCESSION   DATIAL010000011 DATIAL010000000
VERSION     DATIAL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507731"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VLA60_00390"
     CDS             <1..771
                     /locus_tag="VLA60_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HSF07836.1"
                     /translation="DPRILGLVNLRGENIALVDLKQAIGLGPLDPVGAKLIIAEYNDN
                     KQGFLVAGVDRIIRMSWERIQIPPPMVQSSRQGAVTAITKLEDGRMVLILDVEKVLAE
                     LHPRSEEEVFVGIELHEELAGKRVLVADDSLVARKQITKTLERLGMKYEETQTGREAL
                     TRLRQYAELSDKTERTIFDFVGGVITDIEMPEMDGFSLTKAIREDPRLQGLPILMHSS
                     LSGQCNVDKGKALGVTDYVTKFHPTDLRDKLVEHLGKS"
     gene            877..2982
                     /locus_tag="VLA60_00395"
     CDS             877..2982
                     /locus_tag="VLA60_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF07837.1"
                     /translation="MMKPTWNVGITTKLVWILVFFSLIPLSVQVYSLFQTAEVLKKEV
                     GVQYQELAEVIVEKIHFYLAERAADAQILSRNTMSGLSGQLGQAVSHSYELVQVLNAY
                     VQATGMYSLVQVVDLDGHLIAVNDHDAEGLPLQTEELYGKNYQSTPWFRALQRAGERA
                     GPTEPSSRSNVTQEVFVESVMIDRDVQALFPRESGLTIGLSVPLYAQGRVVGYGSHRM
                     KFSRIEAFFQEAYQDLKKAGFPHAELILQDDQGMTLLEYAPAVYGTEEVAHDFDNVVF
                     KANLVQLGLDAAQMAVQGQSGNARNFHPGKQTNQVIGFSPMSGGARSLGLHWSVLVQV
                     PEDEAFSHVRSLTNKTLLEMLVGLLVVVPIGILMGRKVVSRLKPVWEVAAKASKGDLT
                     HRVPVKTQDELGQMGLALNNLLDELSRMLLQTRNVAHSLSQASVQLSSVGHQVVRASQ
                     SQVHQATQVAAAVEEMSATADDMARHTQTLASTATGVNDSAVRGGDVVVSSIHGMESV
                     SSRIQESAGRIQNLGQRSKDIGDIIGVIEDIAEQTNLLALNAAIEAARAGDQGRGFAV
                     VADEVRKLAERTGKATKEIATVIESVQAGTHEAVRSMEAGTEEAHSGMALAREAGNRL
                     TEIVQGVQRVVDMIQHFAESTKQQSAVSGQISSSIQQVAQLSQDNESHIQGVATATKQ
                     FAALAEDLQTSLSRFTLKR"
     gene            3023..>4039
                     /locus_tag="VLA60_00400"
     CDS             3023..>4039
                     /locus_tag="VLA60_00400"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF07838.1"
                     /translation="MQRLSIGPKFLLILGTLAVGLIVCSLVVIYQQEFGRLQMNLEEQ
                     GKIIQAQFEVTRAYIVKNYVGKLKQSSMGSNLHVSRDHEQDPDAIPFPATAIQEIGQD
                     LGVVGVYQARLVSDQPMNPANAPKDSFEQKALERIKNGAKSVSEIETINGVPTFRRAS
                     ADVATVEACVSCHGGKKLGDVLGMLSLSIPITQGKEVMVSSMLHLGMWMIGIIVIFLI
                     AVYWLVCRLVLQPLHALMGTSRAISQGEGDLTERVAVGKGFDEIVELSHDLNSFIEKM
                     RLAVSSVKHVTNRLVGSTVQLSTTSDEVVLAAEGQNARVMQSVFTVEEVTRIAGEVAQ
                     SSTEA"
BASE COUNT         1027 a          827 c         1178 g         1007 t
ORIGIN      
        1 gatcctcgaa ttctcggtct ggtgaatctc cgtggagaaa atattgcctt agtggatttg
       61 aagcaggcga ttggacttgg accgttggat cctgttggcg cgaagttgat cattgcggag
      121 tataacgata acaaacaagg atttttggtc gcgggcgttg atcggatcat tcgtatgtcg
      181 tgggaacgca ttcaaatacc tccaccgatg gtccagtcga gccggcaagg ggcggtcacg
      241 gctattacca aattggaaga tggccgaatg gtgttaattt tggatgtgga aaaggtcttg
      301 gccgagctgc atccacgaag tgaggaagag gttttcgttg gcattgaact gcacgaggaa
      361 ttagccggga aacgtgtttt ggttgccgat gactcgcttg tggccagaaa acaaattaca
      421 aaaacgttgg aacgattggg catgaaatac gaagagacac aaacgggaag agaggcgctc
      481 acccgattac gacagtatgc ggagttgagt gacaaaacgg agcgaaccat ttttgatttt
      541 gtggggggtg tgattaccga catcgaaatg ccggaaatgg atgggttttc gctgacgaag
      601 gctatccgag aagatccccg attgcaaggc cttccgatct tgatgcattc ctccttgtcc
      661 ggtcagtgca atgtggataa aggtaaggct ttgggtgtga cagattatgt gaccaaattt
      721 catcccacgg accttcgtga caaattagtt gaacatcttg gaaagtctta ggaagtcctg
      781 gttgggtcat gtagaaaaaa acgtatcgtc ggaggggatg aaaagaattt actgccatat
      841 aacgagacac atgaaacgtg tattaagagg ataccaatga tgaagccgac gtggaatgta
      901 ggcattacaa caaaattagt gtggattctc gtgttttttt cgttgatccc tctgagtgtt
      961 caggtctatt cattgtttca gactgcagag gttttaaaaa aagaggttgg agttcaatat
     1021 caagagctgg cagaagtgat tgttgaaaaa attcacttct atcttgctga gcgcgctgcg
     1081 gatgctcaaa ttctgagtcg gaatacgatg tctggccttt cgggacaatt gggacaagct
     1141 gtgagtcact catacgaatt agtgcaggtc ttgaatgcgt atgttcaggc aaccgggatg
     1201 tattctctgg ttcaagtggt ggatctggat ggtcatctga ttgcggtgaa tgatcatgat
     1261 gcagaaggct tgcctcttca gacggaggag ttgtatggaa aaaattatca atcgactccg
     1321 tggtttcggg ccctccaacg ggctggagag agggccggcc ctacagagcc ttcttcaaga
     1381 agcaatgtga cacaagaggt ctttgtggaa agcgttatga tagatcggga tgtgcaggca
     1441 ctctttcccc gagagagtgg tttgacgatt gggctctcgg ttcctctcta tgctcagggc
     1501 cgagtggttg ggtatgggag ccatcgtatg aagttttcaa ggattgaagc cttttttcaa
     1561 gaggcatacc aggatttaaa aaaagccggg tttccccatg cggaacttat cctgcaggat
     1621 gaccaaggta tgacgctact ggaatatgct ccggctgtct atggaaccga agaagtggct
     1681 cacgatttcg acaatgtggt gtttaaggcc aatctggttc aattgggact tgatgccgca
     1741 caaatggctg tccagggcca gtccggaaac gccaggaatt ttcatcccgg gaaacagacg
     1801 aatcaagtca ttggttttag tccgatgtcc gggggagccc gatccttggg ccttcattgg
     1861 tcggtgttgg tgcaagttcc agaggatgaa gcgttcagtc atgtcaggag tctgacgaac
     1921 aaaaccctgt tggagatgct ggtgggtctt ctcgtggtgg tccccattgg gattcttatg
     1981 ggaagaaaag tcgtgagccg attgaaacct gtgtgggaag tggcggcgaa agcctcgaag
     2041 ggggatttga ctcaccgggt tcccgtgaag actcaagacg aactcggaca aatgggtttg
     2101 gctctgaata acttgttgga tgaattaagt cggatgctct tgcaaacccg gaacgtcgct
     2161 cactccctgt ctcaagcgtc ggtccagttg tcatcggttg gccatcaggt cgttcgggcc
     2221 agccaatcac aagttcacca agccactcag gtcgctgcgg cggttgaaga aatgtctgcc
     2281 actgccgatg atatggcccg tcacacgcag accctggctt cgacggccac aggagtcaat
     2341 gattcagccg tccggggtgg agatgttgtg gtctcttcta ttcatgggat ggaatcggtt
     2401 tcaagccgga tacaggaatc cgccggccgt attcagaatt taggacaacg ctccaaagat
     2461 atcggggaca ttattggagt cattgaagat attgcggaac agacgaatct gttggcgctc
     2521 aatgcggcca ttgaagcggc cagggcagga gaccagggac gagggtttgc tgtagtcgca
     2581 gatgaggttc ggaaactcgc cgagcgaacg gggaaggcga caaaggaaat tgcaacggtc
     2641 atcgaatcgg tacaggctgg gactcacgaa gcggttcgtt cgatggaagc aggaacggag
     2701 gaagcgcatt ccggaatggc gctggccaga gaagccggaa atcgattaac ggagattgtg
     2761 caaggggttc aacgggtggt ggatatgatt caacattttg ccgaatccac aaaacaacag
     2821 tctgcggttt cagggcaaat ctcttcgagt attcaacagg tggcgcaact cagccaggat
     2881 aatgaaagcc acattcaggg cgtggcgacg gccaccaagc agtttgcggc attggcggaa
     2941 gaccttcaga catctcttag tcgctttacg ctaaaaaggt aatgccatcc tatgttcgat
     3001 gtgtctctcg tgaaggaagt gtatgcagcg tttaagtatc ggtcctaaat tcctattaat
     3061 tctggggacg ctagccgtcg gcttgattgt ctgtagccta gtggtaattt accaacaaga
     3121 gtttggccgg ttgcaaatga atttggaaga acaaggaaag attattcaag cacaatttga
     3181 agtcacgaga gcctatattg tcaaaaatta cgtgggaaaa ttaaagcaat cgtccatggg
     3241 ctctaatctg catgtgtctc gtgatcatga gcaagatccc gatgccattc cttttcccgc
     3301 cactgccata caggaaatcg gccaggattt aggggtggtg ggggtgtatc aggcacggct
     3361 cgtcagcgat cagccgatga atcccgctaa tgccccgaaa gatagctttg aacaaaaggc
     3421 tcttgagcga attaagaatg gagcgaaaag tgtcagtgaa attgagacga tcaacggggt
     3481 tcccacgttt cgacgggcga gcgcagatgt ggctaccgtg gaagcttgtg taagttgtca
     3541 tggggggaaa aaattgggag atgtgttagg catgttgtcc ctttcaattc cgataacgca
     3601 gggcaaagag gtgatggtaa gttcgatgct ccatttaggg atgtggatga tcgggatcat
     3661 cgtcattttc ctgattgcag tgtactggct ggtttgtcgc cttgtcctgc aacctcttca
     3721 cgccttaatg ggcacctctc gtgccatttc acagggcgaa ggtgatctga cggaacgagt
     3781 ggcagtggga aaggggtttg atgaaatcgt ggaactcagt cacgatctca attcgtttat
     3841 tgaaaagatg cgtttggcgg tgtcgtctgt caagcatgtg accaaccgtc ttgtgggttc
     3901 caccgttcag ctttccacga cgtcggatga agtggttcta gctgctgaag ggcaaaatgc
     3961 tcgcgtgatg cagtcagttt tcacggtgga ggaagtgaca agaattgcgg gagaagtcgc
     4021 gcaaagttcc acagaagcg
//
LOCUS       DATIAL010000012         5373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1185191, whole genome shotgun sequence.
ACCESSION   DATIAL010000012 DATIAL010000000
VERSION     DATIAL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185191"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..843)
                     /locus_tag="VLA60_00405"
     CDS             complement(160..843)
                     /locus_tag="VLA60_00405"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF07839.1"
                     /translation="MAHANSDIVETGRLLATLLDAGRVTIGQNQALINDASKGDKGFT
                     PEVFEKQMVALYKERMGIDLHNLQNAQIPDQAKPLLTRLVEESQKTVASYQTVINFEG
                     VKFKGLIPATFGTETARRFQNWSNVYLKQTAPDHFLRNPKNKADAFEREMMQTFATSA
                     PGDRNEVFSKTIEDEKSVRVMLPLYYEKACLICHGGPKGERDVSGYEKEGGKEGELAG
                     AISVKLPLP"
     gene            1350..1655
                     /locus_tag="VLA60_00410"
     CDS             1350..1655
                     /locus_tag="VLA60_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07840.1"
                     /translation="MARAKRFFLLRPLDGLTCGSSGTTVNGCVRGGGGRCFPLSLLPV
                     SVQPLAQTSAEKMLNERAGCMLATPFAFLFNDTNHSLGRKGWAHLISSFAMSSNALA"
     gene            2845..3222
                     /locus_tag="VLA60_00415"
     CDS             2845..3222
                     /locus_tag="VLA60_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07841.1"
                     /translation="MKIVMPVSVMLWIMLISAPLLKAEVIVETSGGEEEGMSLKHHQG
                     NPRPGKAPMRIEEPDGTLRPLPSGSIPESRMSTEGLRDVPDAGGGTMVNVEGRFQSIL
                     KVPDGADQAIHHHEPEDLEPLKP"
     gene            3248..5134
                     /locus_tag="VLA60_00420"
     CDS             3248..5134
                     /locus_tag="VLA60_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSF07842.1"
                     /translation="MKYSKADILRSPSPSQLPIPFGLCVSGFLVVLFFGCFGLLTETF
                     AATIIIVNKDSPGEGFNDPAPFTPVGGNTATTLGEARLNAFEYAANILAQVLVSTVGI
                     RVDARIDSLGAGILGSAGPNTVHRNFPNAPVANTWYVQSLANKLAEQDLDLTTSDISA
                     TFSANFSNWYFGLDANPPSGQFDFVTVVLHEIIHGLGFLSLVDVQTGEKFLGTDDVFM
                     RWLELHGSSPANYPLMTVSQRQYANVASPNLHFVGVNLQEASGTLTAGRTDTHVQMYA
                     PNPVQLGSSVSHFTNTIKPDQLMEPGIASGRAIHDLGLAQPLLQDLGWIFDAGVSFPT
                     RNDVNGDEKADLVWRNTSTWAVAIWLMNGSTIASSGSLGVVSSKWQVAGTGDVNEDGK
                     ADVIWRHSDTGSVAVWQMNGPTVMSVGFPGSASMDWQIASVGDVNGDGRADLVWRNTI
                     SGEVTIWLMNGSMIGSSGHLGAVPLEWRISGTGDMNADGKVDLIWRNDLTGAVEVWLM
                     QGLTVLSVGFPGEASTDWQIAGVGDVNDDGMGDLVWRKTSNGEVAIWLMNGVTLSSPG
                     FFGVVPQGWEIANTSDVNGDGSADIIWRNKQTGTVAVWMMNGLTVMSIGFPGSTSINW
                     KIQY"
BASE COUNT         1354 a         1172 c         1344 g         1503 t
ORIGIN      
        1 aaaggtcgcc aggggaaggc gtgatcatta atgaacgctt ttcttgaaca tatcctggat
       61 gagatttatt cttatttgct tttttctctc caaaatagta ggaggtcatc tttcctcctt
      121 atcaaagatc ggcctctata cgtctttccg aaatggtctc tagggaaggg gtaatttcac
      181 actgatcgcc cccgctaact caccttcctt tcccccttct ttctcgtagc cagatacatc
      241 tctttcacct ttgggccccc catgacagat taagcaagct ttctcatagt agagtggcag
      301 catgacccgt acactctttt catcttcaat ggtcttgctg aaaacttcat tccgatcgcc
      361 aggggcagac gttgcaaagg tttgcatcat ttctctttca aaggcgtcag ctttgttttt
      421 ggggtttctt aaaaaatgat caggcgccgt ctgctttaaa taaacgtttg accaattttg
      481 gaaacgcctg gccgtttcgg tgccaaaggt ggcgggaatg agccctttga atttgacgcc
      541 ttcaaagttt atgactgttt gatagcttgc tacggttttc tgactttctt ctacgagtct
      601 agtcaataac ggcttggctt gatctggaat ttgtgcattc tgcaggttat gcaaatcgat
      661 tcccattcgt tccttgtata acgctaccat ctgtttctcg aatacctccg gggtaaagcc
      721 cttgtcccct ttgcttgcat cattgattaa cgcttgattc tggccgattg tcactcgacc
      781 tgcatccaat aatgtggcca aaagccgacc tgtttccaca atatcggaat tggcatgggc
      841 catcacgggg ctgatccatg cacaccagac cattaacgca ccccatcttc tcaaaactcg
      901 acgggacatc tgtttcatac tgtctctccc taatgaataa ggatatcgca aacttcttgg
      961 gacaacttcc ggatcatccg taaaatgtga acaaggaaaa attagggcgc acgaaaaaaa
     1021 acaattttgg cgtgaagctg aaaggatgct gctttgggac cgccttcacg ctaagcacca
     1081 aagaatacaa gaacctgaaa atctgttcaa ctcagagaag gaagtaggga aagtgggaag
     1141 acggagacgc cggaaatttt tggtaaccca tttgaggcat agaggcataa ataggtagct
     1201 cgcggactac agcaacccca tatgatctta cacgtgacga ctaactaata aatgggcatc
     1261 attgggatat tcagaaaaaa cctgtcaggt acatattcat ggtaaggaag tatgatcgaa
     1321 gttcagtatt gactttatga cccgaatcag tggcgagggc aaagcggttt ttcttgcttc
     1381 ggccgttgga cgggctgacg tgtggcagca gtggcacaac cgttaatggt tgtgtgcgag
     1441 gtggaggcgg tcggtgcttt ccgttgtccc tgttgccggt aagtgtgcag ccactggccc
     1501 aaacatcggc agaaaaaatg ctcaacgaac gggctggatg catgttggcc acgccttttg
     1561 cctttttatt taacgacacg aaccatagcc tgggtcgcaa gggatgggca cacctgattt
     1621 ccagctttgc tatgtcttca aatgcgctgg cgtaaaattc atgggatcgc cgatcacgga
     1681 tactttattg agggggaaga ttagtgtttg gaggcaagat ttaatctcga gagcaagtcc
     1741 tattcccgct tttcaccagg atagataata gctcgctcca gttgaacgag aaaagaacta
     1801 aaaggtttat gcttcaagtg ggctacgaat tgaaagagat aaatgctttt tcccttggca
     1861 tttgcctgcc ccaaaaggtc tatgatgttc tggcgatttc cgcccctaaa cacatgaaaa
     1921 gcttattttt ctcgatttct ataccttgtt aatctttcca caccaaaatt tccacggtaa
     1981 atatcctcga ggaccatggg aagaagtcat gtggctaaat gtccatccat tcttaagaga
     2041 tgcgcatcac gactaaagtc cagagaaaat attgtcgatg aaaaatgcga accttattca
     2101 tatgtatcct gctattcgtt gccgtcatcg gaatcaaatt gacctagacg gtgctccttt
     2161 ccatcttcag aaagggaact cttctctttt tccaaacccc cgtcggacaa caacgaatgg
     2221 tccaatcaga atggagcaat ggcaattcaa ccgctctcct gtttgaagtc atccatccat
     2281 cccaacatag gattcactct caatcttgcc tcgaatttta aatggctagc agataaaaac
     2341 tgctgaatgt tctatggttt ttggggggtc tcaataccaa ttgacccgtt ttcgcttgtt
     2401 aagagaatga agaagggttg ccttcaaatg gtgtatgggt agtttttatc cttcaaattc
     2461 ggaatcataa gtccaagcaa attttggatt ttcctccccc tcatgaattc aagcgaattc
     2521 caattggccg aatccgttgc tttaggacaa aattcgtggc atctacaagc cttgcacaca
     2581 ttctttctat ggagtggttg gaattcttga gatatactaa tttccctcgc tcatgcaccc
     2641 tatacacaac tacacacaca acgtgtcggg aaaacatgac atactttttc aatgaaacac
     2701 catgtttttt tttctttagt tgtcatggac gaaagggaat agtagattga atgacaatcg
     2761 caggcagaat aacttttttg atcggcttcc cgccgaattc tctctcagag agaataaata
     2821 gaacttttga aaggaggaaa tacaatgaaa atcgtgatgc cggtatctgt catgctttgg
     2881 ataatgctga ttagtgcccc actcctgaaa gcagaggtta ttgttgaaac atctggcgga
     2941 gaagaggaag ggatgtccct taaacatcac cagggaaacc ctcgaccagg taaagcccca
     3001 atgcggattg aagagccgga tgggacgcta cgacctcttc cttctggctc gatacctgag
     3061 agtcgtatga gtacggaggg cttacgggat gttccggatg cggggggagg aacaatggtg
     3121 aatgttgagg gccggtttca gtcaattctg aaggtgcctg acggagcgga ccaggcgatt
     3181 catcatcatg aacctgagga cctagagcca ttgaaaccct gaagattgtt ggaagactct
     3241 ttcatccatg aaatattcta aggccgacat actgagaagc ccttctcctt cgcagttacc
     3301 gattcccttc ggcctctgcg tgtccggatt tctggtcgtc ttattctttg gctgctttgg
     3361 cctcttgact gagaccttcg ctgccaccat tattattgtg aataaagata gtcccgggga
     3421 aggatttaac gatcctgcgc cttttacccc agtgggtgga aatacggcca ccacccttgg
     3481 agaggcacgg ttgaatgcct ttgagtacgc ggcaaatatt ttagcgcagg tgctggtgag
     3541 cacggtaggc attcgagttg atgctcgaat tgattccttg ggagccggaa tactgggatc
     3601 ggctggtcct aataccgtcc atcggaattt ccccaatgct cctgtggcca atacctggta
     3661 tgtccaatcc ctcgccaata aacttgcaga acaggacctg gatcttacta ccagtgatat
     3721 aagtgcaacc ttcagcgcaa atttttcgaa ttggtatttt gggctggatg ccaatccccc
     3781 atctggacaa tttgattttg ttactgtggt gcttcatgaa attattcatg gtcttggttt
     3841 tttgtcactt gttgatgtgc aaaccggaga gaaattccta ggaaccgatg atgtttttat
     3901 gcgttggtta gagcttcatg gatccagtcc ggccaattac cccttaatga ccgttagcca
     3961 aaggcaatat gcgaatgtgg cgagtccgaa tcttcatttt gttggtgtca atcttcaaga
     4021 ggcatccggg accctgacgg ctggacgaac agatactcat gttcagatgt acgctccaaa
     4081 cccggttcaa ttgggttcat cggtgtctca tttcacgaat acaattaagc ctgatcagct
     4141 gatggagcct ggaatagctt ccggaagggc gattcatgat ctcgggttag ctcaacctct
     4201 gctgcaagat ttaggatgga tatttgatgc tggtgtgagt ttcccaactc ggaatgatgt
     4261 caacggtgac gagaaggcag acctcgtctg gcgcaacacg agcacgtggg ctgttgcgat
     4321 ttggttgatg aatggttcta caattgcttc ctccggttca ctgggcgtgg tgtcgtcgaa
     4381 gtggcaggtt gccggtaccg gagatgtcaa tgaggacggg aaagcagatg tcatttggcg
     4441 tcacagcgat acaggatcgg tagcggtgtg gcagatgaat ggtcccactg tcatgtcggt
     4501 gggttttccg ggtagtgcct ctatggattg gcaaatcgcg agtgtgggtg atgtcaacgg
     4561 agacgggagg gcggacctcg tttggcgaaa cacgattagt ggggaagtga cgatttggct
     4621 aatgaacggc tcaatgattg gttcttccgg ccacttgggc gccgtgccgt tggaatggcg
     4681 aatatccggg actggagata tgaatgccga tggaaaagtg gatcttattt ggcggaatga
     4741 cctgactggc gcagtggaag tgtggcttat gcaagggctc acggtcctgt cagtaggttt
     4801 ccctggagag gcttccacgg attggcagat tgcaggtgtg ggtgatgtga atgatgacgg
     4861 gatgggggac ctggtttggc gtaaaacgag taacggggag gttgcaattt ggttgatgaa
     4921 tggggtgact ctcagctcgc cgggtttctt tggcgtggtg cctcaggggt gggagattgc
     4981 gaacaccagt gatgtcaatg gggatgggag cgcagatata atttggcgta acaagcagac
     5041 gggtactgtg gcggtgtgga tgatgaatgg gctcaccgtc atgtcgattg gctttccagg
     5101 cagtacctca atcaattgga aaatccaata ttagggaagg ttaaaaaaga aatctacaac
     5161 cctgtgttct acccaacaca cagaaaatga cctaaatggt tgtttcctgc tgatggttga
     5221 atttgccttt tcctaatggc aagttatgat ttccgggtgc attgttttca agcggtcata
     5281 ttatttcgag ccattcttgg ctgagattag tccaattggg aatgaatgat ctggtgatga
     5341 caaacttttt ccattgtagg tgagtatgga tat
//
LOCUS       DATIAL010000013         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_325479, whole genome shotgun sequence.
ACCESSION   DATIAL010000013 DATIAL010000000
VERSION     DATIAL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325479"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VLA60_00425"
     CDS             <1..560
                     /locus_tag="VLA60_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSF07843.1"
                     /translation="GYVVLVPNLYGRQGGMVTANAEVADALMERVNEQQALQDINASF
                     EFLNANLTEDTTLERTTRNAHAVIGFGMGAALAIKTAAQRRRLQAAVALCGTLPADAE
                     IAQRLYCPLLLQMPGKDSPISPEELERFCQTAKQAGKTVEVRSYPEASNGFWQSGSPH
                     YRVSDAEEALQTSMDFINKIIKKIP"
     gene            689..1801
                     /locus_tag="VLA60_00430"
     CDS             689..1801
                     /locus_tag="VLA60_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07844.1"
                     /translation="MCLHLIIFGFHPAFANQTLAGPPWSFLEQHREQILQLSSDDTAL
                     VFFQSIFPPKNQISRTRTQSPSSTKNVTLSEEVKEAAILYLASLAVTAHARTIQKHIV
                     QLSPTNPLTSGGIPGEPQLKWMMKQTPLQTLKPMIDFYTQVSAWSQAAASQPHPSGNE
                     YAAFASYYDQTYPDWDDSPLSWTNLYSQDGQKGIEARLFEYWQTPDHPADQHPSPSTK
                     NAYAQYYIGTRLLPMFRANLLAQSIRVETMAYETSWKSWHDIQEWQQQEQTRSATARL
                     CGTWQWVVHNHQNHGDHKMVITFSSLEQSSPSQPQPTTITIQGDTVYLKWTFPQGFQE
                     DSLLLSNRDTHLEGTFKNSLGPHGSISGKRLTSCQS"
     gene            1886..>2434
                     /locus_tag="VLA60_00435"
     CDS             1886..>2434
                     /locus_tag="VLA60_00435"
                     /inference="COORDINATES: protein motif:HMM:NF037039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07845.1"
                     /translation="MVVHDSLDSDIRRFGQTLAEHAHRHTPGFWDHRWWTNLLLDWGT
                     RDEQFKVQLFRFIDVLPALHTDTQFVRILKEYFQDLPSLPTPLRWLLTRFSNNPLAAQ
                     VGTRLLRRQFLKMAYTFMAGETVEQALPTLARLWHARCACSIDLFGEATVSEVEADRY
                     HQRCLQALTQLHQVAQQWDSHPL"
BASE COUNT          629 a          683 c          524 g          598 t
ORIGIN      
        1 aagggtacgt ggtcttagtc ccgaacctgt atgggaggca gggcggaatg gtgacagcca
       61 atgctgaggt cgccgatgca ttaatggagc gggtaaatga acaacaagcc ttacaggata
      121 tcaacgccag ttttgaattc ctcaatgcca atctgaccga agatacgaca ttggaacgaa
      181 ccactcgcaa cgcgcatgcc gtaatcgggt ttggtatggg ggcggctctt gccatcaaaa
      241 ctgccgctca gcggcgacga ctccaagccg ccgtagccct ctgtggcacc cttcctgctg
      301 atgctgaaat cgcccaacgc ctttattgcc cgctattgtt acaaatgccg gggaaggatt
      361 ctcccatttc cccggaagaa cttgaacgat tctgtcaaac agccaaacaa gccgggaaga
      421 ccgtagaagt acgatcctat cctgaggctt ctaatgggtt ctggcaaagc ggctctcctc
      481 attatcgagt gtcagatgcc gaagaagccc tgcaaacctc aatggacttt atcaacaaaa
      541 tcatcaaaaa aatcccatag gcgaattttt gattttggac aaatctaccc tatttatttc
      601 tcctcttcca caatcgccac aaacctcagt tttgacaaaa tccttctcac atcgctccaa
      661 ttggaattgg gtaagggtcc tatgtatatt gtgccttcat ctgatcattt tcggttttca
      721 ccccgctttc gccaaccaga cattggccgg acctccctgg tctttcctgg aacaacaccg
      781 ggaacagatt cttcaacttt cctctgatga cacggctctc gtcttttttc aatccatatt
      841 tccaccaaag aatcagatct cgcgaacccg cacgcaatct ccatcctcga caaaaaatgt
      901 cacactatca gaagaggtca aagaggctgc cattctctat ttggcatcgt tggcggtaac
      961 tgcacatgct cggactattc aaaaacatat cgttcagctg tcaccaacga atccactaac
     1021 ctctggagga atccccggag aacctcaact caaatggatg atgaaacaga ctccacttca
     1081 gactctgaaa cccatgatag atttctacac acaggtgtct gcctggagcc aagctgctgc
     1141 ttctcaaccc catccttccg gtaacgagta tgcggcattt gcatcctatt acgatcaaac
     1201 ttacccggat tgggatgata gccccttatc ctggacaaac ctgtattccc aggatggcca
     1261 aaaagggatt gaggcgagac tcttcgaata ttggcagaca cctgatcatc cggccgatca
     1321 gcatccgtca ccatccacta agaatgccta tgctcaatac tatatcggta cacgacttct
     1381 gccgatgttc cgggcaaacc tgctcgcgca gagcatccgg gtagagacca tggcctatga
     1441 aacatcctgg aaatcctggc acgatattca ggaatggcag cagcaggaac aaacccgatc
     1501 agctacggct cgcttgtgcg gaacctggca atgggtggtg cataaccacc aaaaccacgg
     1561 ggatcataaa atggtgatca ccttttcatc tcttgaacaa tcttcgccgt cccaacccca
     1621 accgaccaca ataacgattc agggggatac ggtctatctc aaatggacct tcccgcaggg
     1681 ctttcaagaa gacagcctgc tgctcagtaa tcgtgacaca catctggaag gcacatttaa
     1741 aaactctctc ggccctcatg gcagtatttc cggtaagcga ttgacttctt gccaatcata
     1801 aggcctcttc ccgtctcttc aatcctttat cccgctcccc tcttctccga aaaggtgtaa
     1861 aatatacatg gaaggagggc gaggtatggt tgtgcatgat tcattagatt cagacatacg
     1921 gcgcttcgga cagacgttag ccgaacacgc tcatcggcat actccgggat tttgggatca
     1981 ccggtggtgg acaaatcttc tcttggattg gggaacgcgc gatgaacagt tcaaagtgca
     2041 attatttcgc ttcatcgatg tacttcccgc ccttcacaca gatacccaat tcgtgcgcat
     2101 cttgaaagaa tattttcaag acctgccatc cctcccaacc cccctgcgct ggcttctgac
     2161 ccgtttttcc aacaaccctt tagccgctca agtgggcaca cggctgttgc gacgacaatt
     2221 tcttaaaatg gcctacacat ttatggcagg cgagaccgtt gaacaagcct tgccgacact
     2281 cgctcgtctt tggcacgccc gatgtgcttg ttccattgat ttgttcggag aagccacggt
     2341 cagtgaagtt gaagctgatc gctatcacca acggtgtctt caggcattga cccaattaca
     2401 tcaggttgct caacagtggg attctcaccc tctt
//
LOCUS       DATIAL010000014        14028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1185435, whole genome shotgun sequence.
ACCESSION   DATIAL010000014 DATIAL010000000
VERSION     DATIAL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185435"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /gene="lptD"
                     /locus_tag="VLA60_00440"
     CDS             complement(<1..1459)
                     /gene="lptD"
                     /locus_tag="VLA60_00440"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HSF07846.1"
                     /translation="MTCASLGDDLRVFWRRSHGWLLGLVIFITILAAGETVFGQSDVS
                     VVESKEFPAASSAEKGSAEPVSQGVPVAINADRIEYDNDREEYHASGAVDIIRGPIHL
                     NADEATLQKLTGRLTARGHVHLRDEKTDIWSEELELNLNTEAGVITNGNIFLKEQNSF
                     VTGRRLQRFSETHYRVKEGSFTNCDAKNGEIPAWRFNFHDMDVEYEDSLFGKGVWFNV
                     NDVPVVPLPTFRYPLGASRKSGFLFPTTGYNTNFGYQYRQGYFWAINPSNDLTISPQI
                     LTARGGGADLEYRYMWSRLTKGEWLVRSLYDTQQNRGRAEVRGAHVQQFNPDLSLRMR
                     ANFSSDRTVFQNFSSSGTQRALPSQESHLTILQRLDHGALYLSGQYIQPLSTGGSTTF
                     QRLPEIGHRFVNPSVLGSPISLTAETTAVQFHRQTGFDVGRIDVMPGLSMEGLHLGHA
                     LGFRPQVKFREVGYSRGLTDKSAQHRETFWAGAELF"
     gene            complement(1456..2796)
                     /locus_tag="VLA60_00445"
     CDS             complement(1456..2796)
                     /locus_tag="VLA60_00445"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HSF07847.1"
                     /translation="MIRNLLPFIVSRPVSIPVASYPETLDYLFSLHRFGIKLGLEAIN
                     DILARLGNPQRRYPTLHVAGTNGKGSSSAMLARILQASGYRVGLYTSPHLIDFRERIR
                     VQGTDISEESVCALTEQIRRTANPLTTLTFFEFTTALAFLHFQNQQVDIAVVEVGMGG
                     QFDATNVMSPMGVLITGISFDHEAYLGDSLQKIAREKAGIITHKAPVVLGSMPEEILQ
                     IFESQARTWQAPCFRMGQEFFLLETSATTFTYQGPRWTIPDLQTNLPGRHQMVNATNV
                     LAVLEMAVDGTFPISPSAIRTGLQDVRWRGRLEIVQRNPLLLLDGAHNLAGAQVLFDF
                     LHSQIHDGAGRKLILVVGMMRDKNLQGFLRVLIPLVDHLILTQPRMERAASVEELQQA
                     VDRDDLVPCLLADSWEAVCRAKDIAKPADVICVTGSLFLVGEVLNRLTQPGSRT"
     gene            complement(2898..3758)
                     /gene="accD"
                     /locus_tag="VLA60_00450"
     CDS             complement(2898..3758)
                     /gene="accD"
                     /locus_tag="VLA60_00450"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HSF07848.1"
                     /translation="MGWFKRNKNTDPEGTSIKIVEGMWVKCAVCRAIIFKREVERNLK
                     VCPKCQYHFPLSVGERLELIVDPGSFEEWDGDLVPGDPLQFVDTLPYRKRIQKAQEKT
                     GKKEAILTGRCQIGGKPVALGIFDFSFMGGSMGSVVGEKICRAIDHALTEHMPFLLVT
                     TSGGARMQEGTLSLMQMAKTASALAKLQEARIPFVVLLTDPTFGGVTASVAMLGDIIL
                     AEPKALIGFAGPRVIEQTIKEHLPEGFQRAEFLLEHGMVDHIVERKTLKPTLETLLAH
                     CVTEVSSPSG"
     gene            complement(4026..5108)
                     /gene="moaA"
                     /locus_tag="VLA60_00455"
     CDS             complement(4026..5108)
                     /gene="moaA"
                     /locus_tag="VLA60_00455"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HSF07849.1"
                     /translation="MGGGTPIMDGGGAVTLPDAVKDVWGRPLRSLRVSVTDRCNLRCQ
                     YCMPEENYVWLPRENLLTFEEIAFLTNVFSDRGIDRVRITGGEPLLRNNVAELIKMLR
                     QNSRIHDIAMTTNGVLLSEQAQALFDAGLHRVTVSLDTLKPERFKALTRRDTLGRVLE
                     GITSVGRVGFTGLKLDTVAMKGYNEDEVIPLIEYGKQVKGEVRFIEYMDVGGANDWSA
                     EKVLSRKEILKMVGDHYGAVEPVEEVSSAPAQRFRLPDGTTFGIISSTTVPFCSTCDR
                     SRLTADGMWYLCLYAKTGLDLRKLLRMERSREDILSIIQSVWESRKDRGAEERKELEQ
                     VSGRGRYIDIERLRQDPHLEMHARGG"
     gene            complement(5087..5530)
                     /locus_tag="VLA60_00460"
     CDS             complement(5087..5530)
                     /locus_tag="VLA60_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HSF07850.1"
                     /translation="MAVKLEESMLVRVQLENFSIDEELHRVRSRSRRIGGIAMFLGTA
                     RNRSKGRDVSSMSFDHYEGMAQKKLKEIREQALRDFAIIEALVLHRYGPIEIGENIVL
                     IIVGAEHRADAFKACQWCIDELKKITPIWKMETTPEGEVWVEEHP"
     gene            complement(5553..5813)
                     /locus_tag="VLA60_00465"
     CDS             complement(5553..5813)
                     /locus_tag="VLA60_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSF07851.1"
                     /translation="MKIKLFGMLKTMIPGGKDLEVAFSQGEQVKDLVHTIQREYPQVG
                     ELLEKKKVLVSVNQEIAHWDTVLDTADEVALLPPFAGGSGEL"
     gene            complement(5861..6364)
                     /gene="moaC"
                     /locus_tag="VLA60_00470"
     CDS             complement(5861..6364)
                     /gene="moaC"
                     /locus_tag="VLA60_00470"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HSF07852.1"
                     /translation="MANLTHFNESGRARMVDVTDKPPTERVAVAEGKVFVLPETLEKI
                     HAGRIAKGDVLAVAQVAGVMGAKRTPDLIPMCHPLLLTGVDVDFKEDSQPNSDGRCVI
                     TIVATVKTTGNTGVEMEAMTAVSVAALTIYDMCKAVDKGMEFGEIALVSKSGGKSGTY
                     VRMQKSG"
     gene            6531..6884
                     /locus_tag="VLA60_00475"
     CDS             6531..6884
                     /locus_tag="VLA60_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSF07853.1"
                     /translation="MESTTNIANPILANKKLGLLLSTSPEHPNAETVYHLSKTALANK
                     VDTYLYFIDEGVKNLEDPRFSELAKNGLKLFVCAYGCQQHHISTDGYGKEVTFCGLVI
                     LSNIIDGCDRFLAFN"
     gene            7155..9680
                     /gene="shc"
                     /locus_tag="VLA60_00480"
     CDS             7155..9680
                     /gene="shc"
                     /locus_tag="VLA60_00480"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSF07854.1"
                     /translation="MLTSTRHLILETAIDLLGRNGVSALTLEHVAREAGISKGGLLYH
                     FEGKEQLLAGLIELLIQDLDRKQVGEFLTSSIHEEHPGMAFSHEIIDNPVHNRSLRGE
                     EIASILMAAFSINPHLLEPIRERYQNWQSIFLANTLDPTRSLLGRLAVEGLWFSEALG
                     LAPPTREQRATFVLEIQSHTQSGEPMRDTSATTIPTQPNDAFAELASFKSHVEELERQ
                     AYQPTQTSARALLAKQNPQGFWQGDLTADTTLESDYVLLLLWLYPPENGVWEPRIQTK
                     VKEAIRTILDRQLSDGGWNIYTEGPAEVNATTRAYVALRVGGYDPDQPLMQRARERIL
                     ALGGLQATNSYTKNNLSMFGLFPRKYTPSVPPELVLIPGNVLSEISSWSRAIVVPLSI
                     LQASGIQRKVPGDWTVDELMVPNRKLRFPRKDPFSLMFHQVDKILKLWEKRGFKDVRA
                     KAIREAEKWMLDHMRFTDGLGAIFPGMMYALMAMDALGYERDHPDFIDTLGQLEGLIL
                     EKEDALQFQPSLSPVWDTAISMFALGELGVGEPQAMQRAADWLLSKEIRRKGDWSVKR
                     PDLQPGGWPFQFANELYPDIDDTAMVLLALQHAKASDPDRQTRAEGRAINWLLGMQSS
                     DGGWAAFDVDNNWALLNKVPFADHNAMLDPSCPDITGRVLEALCRRDYTYQNPAIARA
                     VQFLLGHQQPNGSWDGRWGVNYTYGTFLAVRGLRASGSPTANAAIQRAATWVRSIQNQ
                     DGGWGESCASYEKQEFVPASSTPSQTAWALLALEAAGDRTSKPVHRGIDWLLTHQNQQ
                     GGWDEELMTGTGFPNVFYLKYHLYSHYFPLLALATWQAGLKNS"
     gene            complement(9881..10885)
                     /locus_tag="VLA60_00485"
     CDS             complement(9881..10885)
                     /locus_tag="VLA60_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSF07855.1"
                     /translation="MALMHQDLVPPDDVEHADLAEVEWKTEFDVVSTLRDLGHEVMAV
                     GVRDDLSVIDKLVTDWRPHIAFNLLEEFNGNPEFDQNVVSYLELLGVPYTGCNPKGLV
                     LTRDKGWSKKIMAYHGIRCPEFVVYPLGRVIKWAEEFPFPVIVKSISEEASLGISQAS
                     IVHDEDKLKERVEFVHQSVGTSAIVERYIEGRELYVGVLGNRRLQALPVWELHLKNLP
                     PDALPIATARVKWSTKYQKKYGIQSGEVEGLHPKLVRHIQQTAKRVFHILGLNGYARM
                     DFRLDPQEHLYILEANPNAQLAYGEDLAESAERAGISYEALIQRILNIGLSWKPESQR
                     "
     gene            complement(10900..11955)
                     /locus_tag="VLA60_00490"
     CDS             complement(10900..11955)
                     /locus_tag="VLA60_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07856.1"
                     /translation="MSLDLLPDFQPPVPAEPEWVSWTDEQLLNLRLSDLKIRISGTEL
                     GQCIRQLYRELKDHGLVFRPHVWLSDDWYSPDGTPGLAVPFYMGHPRLAKLELNQMLE
                     VEGGTPEWCMRILRHETGHAIENAYRLRRRRSRQQMFGHTSVPYPDCYLPKPYSKSFV
                     LHLDMWYAQSHPDEDFAETFAVWLTPHSHWRERYAGWPALKKLEYMDGLMKELAGVAP
                     IVTTREQVDPIQRLHKTLREHYQDKRERYGLDYPNFYDRDLRRLFSGDPDQSANPSAE
                     NFIRKFRKEVRRKVAAWTGEYQYTIDQVLQDMMAHCRERNLRLAVPEEQAKVDFTILL
                     TVQTMNYLHGGRHRVVL"
     gene            12380..12532
                     /locus_tag="VLA60_00495"
     CDS             12380..12532
                     /locus_tag="VLA60_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07857.1"
                     /translation="MYRCEKCQGTMLLDREVDMESGMSLLVFWCINCGLRKQAERAPI
                     PLIEVS"
     gene            complement(12812..13348)
                     /locus_tag="VLA60_00500"
     CDS             complement(12812..13348)
                     /locus_tag="VLA60_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSF07858.1"
                     /translation="MEMTLLLNATYEPLRVVHWQKAINLLWQGKVEVLEFYDRDIRGV
                     SISFKLPSVMRLLNMVKLRSNHHAVKFSRINIFTRDRYTCQYCYARFRTEELTFDHVV
                     PIAKGGKKTWENIVTACWRCNNRKSGRVPHEAGMKLLKEPVKPKWTPRLTLMIGIRHA
                     PESWRDYLYWNVELDADS"
     gene            13497..13835
                     /locus_tag="VLA60_00505"
     CDS             13497..13835
                     /locus_tag="VLA60_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HSF07859.1"
                     /translation="MSLNFHPVAKIQDLPPGTCQSIELQDQGIALFNVEGVIYALDNT
                     CPHAGGPLGEGFVEGDFVECPWHGWKFDIKTGVCQKNPSPSWNVPCYEVQVVDGIIHV
                     AQPTSENRQL"
BASE COUNT         3650 a         3843 c         3150 g         3385 t
ORIGIN      
        1 agaacaattc agcgccggcc cagaaggttt cacgatgttg cgctgatttg tcggtgagac
       61 ctcggctata acctacttca cgaaatttca cctggggacg gaatcccaat gcatgcccca
      121 gatggagccc ttccattgac agtcccggca tcacatcgat acgaccgaca tcgaatcccg
      181 tctgcctgtg aaattgcacc gcggtcgttt ccgccgtcag cgatatggga ctgcccaaga
      241 cactgggatt cacgaagcga tgcccgattt cgggtaaccg ctgaaaggtc gtactcccgc
      301 ctgtgcttaa cggctgaata tattgaccag aaagatacag cgctccatga tcaagccgtt
      361 gcaaaatagt gagatgcgac tcttgactgg gtaaagcccg ttgagtcccg gaactgctga
      421 aattttgaaa gaccgttcga tcacttgaaa aattggcgcg cattcgtaag gacaaatcag
      481 gattgaattg ctgaacatga gccccacgaa cttcggctcg cccacgattt tgctgggtat
      541 catagaggga acgtaccaac cattctccct ttgtgagccg actccacata tagcgatatt
      601 ccagatccgc gcctcctcca cgcgcggtta atatctgagg ggaaatggtt aaatcattac
      661 tggggttgat ggcccaaaaa tatccttgcc gatactgata accgaagttg gtattatacc
      721 cggtcgttgg aaacaagaat ccgctttttc gagaagcgcc caacggatat cgaaaggtgg
      781 gaagcgggac caccggcaca tcgttcacat tgaaccatac gcctttgcca aacaaactgt
      841 cctcatattc cacatccata tcatgaaagt taaatcgcca ggctggaatc tcgccattct
      901 tcgcatcgca attggtgaag gatccctcct tgacccgata atgggtttcg gaaaaccgct
      961 gaagccgtcg ccccgttaca aaggaattct gctccttgag aaaaatattg ccgttcgtga
     1021 tcacacccgc ttcggtattg agattgagct caagctcttc cgaccagata tcggtcttct
     1081 catctcgtaa atgcacatgc ccacgcgccg tgagacgccc cgtgagcttt tgaagggtag
     1141 cttcatccgc attaagatga atgggtccgc gtatgatatc cacagccccg gaagcatgat
     1201 attcttcccg atcattatca tattcgattc ggtcggcatt aatggcgacg gggactccct
     1261 gtgagactgg ctcggcagag cccttctcag ctgaggaagc ggcaggaaat tctttggatt
     1321 ccacaacgga gacatccgat tgcccgaaca cggtttctcc cgctgccagt atcgtgataa
     1381 agatgactag accaagcaac cagccatgag atcggcgcca gaaaacacgc aggtcatctc
     1441 ccaggctcgc acacgtcacg ttcgggaacc cggctgagtg agccgattga gtacctcccc
     1501 aaccagaaat aacgagccgg tcacacagat cacgtctgcg ggtttcgcga tgtctttggc
     1561 cctgcaaaca gcctcccatg aatctgcaag tagacacggc accaggtcat cgcgatcgac
     1621 ggcctgctgg agctcctcta ccgacgccgc cctttccatc cgtggctggg tgagaatcag
     1681 gtggtcaaca aggggaatca aaacacgcaa aaatccctga agattcttat cgcgcatcat
     1741 cccaaccacc aaaatgagtt ttcgtcccgc gccatcatga atttgtgagt ggagaaagtc
     1801 aaataacacc tgagcacccg caagattatg cgcgccatcc agaagaagaa gtggatttcg
     1861 ttgcacgatt tctaagcgac ctctccatct cacgtcttgc aaaccggtcc ggatggccga
     1921 cggtgaaata gggaatgtgc catcgacggc catctccaat accgccagaa cattcgtcgc
     1981 attcaccatc tgatgccggc cgggcaaatt cgtctgtagg tctggaatag tccatcgcgg
     2041 cccttgatag gtaaaagtcg ttgcgctggt ttccagaagg aaaaactcct gacccatccg
     2101 aaagcacggt gcctgccacg tcctggcctg gctttcaaaa atctggagaa tttcttccgg
     2161 catggacccc aaaaccaccg gcgccttgtg tgtaatgatg ccggccttct ccctcgcaat
     2221 cttctgcaga gaatctccca aataggcctc atgatcgaaa ctaatccccg ttatcaaaac
     2281 acccatgggg ctcatcacat tggtcgcatc aaactgtccg cccattccga cttctaccac
     2341 cgcaatgtcc acttgttggt tttgaaaatg tagaaaagcc agcgcggtcg taaattcaaa
     2401 aaaggtgaga gttgtgaggg gattggcggt tcgccgtatc tgctcggtca gggcacagac
     2461 cgattcttcc gaaatgtctg tcccctgaac acgtatgcgc tctcggaaat caatcaaatg
     2521 cggggaagta tacagaccaa cccggtaccc gctcgcttga aggatcctgg ccagcatggc
     2581 tgaggaggaa cccttcccgt tcgtgccggc aacatgcagg gtaggatatc ggcgctgagg
     2641 attccccaac cgtgcaagaa tatcgttaat ggcttccagg ccaagcttga taccaaaccg
     2701 gtgcaaggag aaaagatagt caagcgtttc tggatagcta gccacaggaa tcgaaaccgg
     2761 gcgggagaca ataaagggaa ggagatttcg tatcaataat aactacaacg tatggctctc
     2821 accatgaacg ggatagcatc catcttcaag gctgttgaga tccacctggg cacaaggaga
     2881 gcctgacctt tgggcgatta ccccgaaggg gaagagactt ctgtgacgca atgcgcaagc
     2941 aacgtttcca gggtcggctt gagagttttt cgttccacaa tatgatcaac catgccatgt
     3001 tccaacaaaa actcagcacg ttgaaagcct tcaggcaaat gctctttgat cgtttgctct
     3061 atgacccgtg ggcctgcaaa cccaatcagc gccttcggtt ccgctagaat gatatcccct
     3121 aacatcgcga cactggcagt gacccctcca aaggtcggat ccgttaacag gacaacaaag
     3181 ggaattcgag cctcttgtaa tttcgccaag gctgaggctg ttttggccat ttgcatgagt
     3241 gacaaagtcc cctcttgcat tcgtgcccca ccggaagtag tgaccaacaa aaatggcata
     3301 tgctcggtca gcgcgtgatc gattgctcga cagattttct cgccgaccac cgatcccatg
     3361 ctgccaccca taaatgaaaa gtcaaaaatc ccaagcgcaa cgggtttccc gccaatttga
     3421 catcgcccgg tcaaaatcgc ttcttttttc cccgtttttt cttgagcctt ttgaatgcgc
     3481 ttgcggtacg gcaaggtgtc aacaaactgt aggggatccc ctgggactaa atcgccatcc
     3541 cattcctcga atgatccggg atcgacgatc aactccaatc gctcaccaac cgatagcgga
     3601 aaatggtatt ggcatttcgg gcagactttt aaatttcgct ccacctcgcg tttaaaaata
     3661 atggcgcgac agactgcgca tttaacccac atcccttcca cgattttaat ggacgtgcct
     3721 tcaggatccg tgtttttatt tcgtttaaac catcccatag atcagtctat ccttaagctt
     3781 ctctcatgaa cgcaatgtac gcgaacctac atccagtttt ggctgagaga aagaaagttc
     3841 gagaaaggca ggaaaggaca caacaaaaaa acatatactc cgacacagtc ttcagtcacg
     3901 acacgacatt accataggga ggttgaaccg gcaagaatcc ctcggataag gtgcctaccc
     3961 cgcaaaccat tccggagtat cagcagagtg aggacacgac aggaaacaag gagaaggcac
     4021 gagggtcaac caccccttgc atgcatttcc aaatgtggat cttggcgcag ccgttcaatg
     4081 tcaatatacc ggccacgacc gctgacctgt tcaagctctt ttcgttcttc cgccccacga
     4141 tcttttcgag attcccagac agactgaatg atggaaagaa tgtcttctcg tgatcgttcc
     4201 attcgaagaa gcttgcgaag gtcaagacct gttttggcat acaagcacaa ataccacatc
     4261 ccatcagccg tcaaccgact cctgtcacag gtcgaacaga aaggcacggt ggtggaagaa
     4321 ataataccaa atgtcgtgcc atccggtagc cgaaatcgct gggcaggagc cgaactgacc
     4381 tcctccaccg gttcgactgc cccataatgg tctccgacca ttttcaagat ctctttccgg
     4441 gaaagaacct tttccgccga ccagtcattg gctccgccga catccatata ttcgataaac
     4501 cgcacttctc ctttgacctg cttgccgtat tcaatcaagg gaatgacttc atcctcgttg
     4561 tatcccttca tggcgaccgt atccagcttc aaccctgtga acccgacccg tcccactgaa
     4621 gtaatgcctt ccaacactct gcccagggta tcgcgtcgag tcagtgcttt aaaccgctcg
     4681 ggttttaacg tatccagact taccgtgacc cgatggagcc cggcatcgaa aagggcctgt
     4741 gcctgttcac tcaacaacac cccattggtg gtcatcgcaa tatcgtgaat gcgagagttt
     4801 tgacgaagca ttttgatgag ttccgccacg ttattgcgta atagaggctc gcctccggta
     4861 atacgtactc gatcaattcc ccgatcggaa aacacattcg tcaggaaggc gatttcttca
     4921 aatgtcaaca gattttcccg aggaagccac acataatttt cctcaggcat acagtactga
     4981 caccgtaaat tgcatcgatc ggtcactgac acccgcaagg atcgtaaggg acgtccccag
     5041 acatctttaa ccgcatccgg cagagtcacg gctccccccc catccattat gggtgttcct
     5101 ccacccacac ttcgccctcc ggcgtcgttt ccattttcca gattggcgta atttttttga
     5161 gttcatcaat acaccattgg caagccttaa acgcatcggc acgatgttca gctccaacga
     5221 tgatcagcac aatattttca ccaatttcaa tgggaccata gcgatggagg accaatgcct
     5281 caataatggc aaaatcacga agtgcctgtt ctcggatttc tttgagtttt ttctgggcca
     5341 tcccctcata gtgatcaaaa ctcatggaac tcacgtctcg acccttggac cgattacgag
     5401 ccgtacccaa aaacatggca atacccccga ttcgacggga ccgagaccgc actcgatgca
     5461 attcttcatc aattgaaaaa ttttccagtt gcacccgaac cagcatactt tcttctaatt
     5521 taacagccat cggattgccc tttctcctaa gctcataatt cgcctgatcc tcccgcaaag
     5581 ggaggcaata atgcgacctc atcagccgtg tccagaaccg tgtcccaatg agcaatttct
     5641 tgattcaccg acaccagcac ttttttcttt tcgagcaatt caccaacctg aggatactcc
     5701 ctctgtatgg tatgaaccag atccttgacc tgttcacctt gggaaaaggc cacttctaaa
     5761 tccttgcctc caggaatcat ggtctttaac atcccgaata atttaatctt cacaatttac
     5821 aactcccatg accaagctcg aagaagggtc atcatctttc ctacccgctt ttttgcatac
     5881 ggacatacgt tcctgacttt cctccggatt ttgaaaccag ggcgatctct ccaaactcca
     5941 tgcctttatc taccgcctta cacatatcgt aaatcgtcag agctgccacg gaaacagctg
     6001 tcatggcctc catttcgact ccggtgtttc cggtggtttt caccgttgcg acaatcgtaa
     6061 tgacacaccg tccgtcagaa ttaggctggg agtcttcttt gaagtcaaca tccactcctg
     6121 tgagaagtaa tggatgacac atcgggatca gatccggagt ccgtttggcc cccatcacgc
     6181 cggccacttg agccacggcc agcacatcac ccttcgcaat ccgacctgca tgaatttttt
     6241 caagggtttc cggcaagaca aacactttcc cctctgccac cgctacacgc tcggtcggag
     6301 gcttgtccgt cacatccacc attcgagccc gtccggattc attaaaatgc gtaagatttg
     6361 ccatcatcaa aaataatcag agatgaaaat ttctcttgaa aatcagtaaa ttataaagac
     6421 agaggaaaaa cgacgtcaag caaaagatcc ctcctgaata ctcccttgac agccaagctc
     6481 attccaccta taaacagtcg taaaacctta ttattcagag agtttctccc atggaatcca
     6541 ccaccaatat agccaatcct attctcgcca acaaaaaatt aggcctgctg ctctcaacct
     6601 cacccgaaca tcccaatgca gagacagtct accacctctc caaaaccgcg ttagctaata
     6661 aggtggatac ctatttgtat tttattgatg agggagtcaa aaacctagaa gacccgagat
     6721 tttcggagtt ggcgaagaac ggactgaaac tatttgtgtg cgcctatgga tgccaacaac
     6781 accacatctc aaccgacggg tatggcaaag aagtgacctt ttgcggcttg gttattctct
     6841 caaacatcat cgacgggtgc gaccgttttc tggccttcaa ttaactttcc gctcaactcc
     6901 cttctctgag tctctgccaa atggtacaaa gtcccagata ttcgaggcag ttattttgtg
     6961 gaccaaaatc atttcaaatt tcctattctt tccttgacct caggaagagg catgattgga
     7021 attgtgaatg ccattttcct tttttgctgc ccggccttta atccagccca gaatcgtcct
     7081 ccctgcgtac cagcgtgtaa aatcaaaaaa tttccaattg aaactaaacc agccagacgg
     7141 tacagtagac agcaatgtta acttcaaccc ggcatttgat tctcgagacc gccatagacc
     7201 tgttgggacg aaacggcgtt agtgcgttga cgctcgagca tgtcgctcga gaagccggaa
     7261 tcagtaaagg tggcttactc tatcattttg agggaaagga acagttactc gcggggctca
     7321 tagagttact catacaggat ttggatcgca aacaggttgg agaatttctc actagctcca
     7381 ttcatgagga gcatcccggc atggctttta gccatgagat tatcgacaat cccgtccata
     7441 atcggtccct tcggggagaa gaaattgcct ccatcctcat ggcggctttt tccatcaatc
     7501 cccatctact cgagcccatt cgagagcgat accaaaattg gcagagcatt tttctggcca
     7561 atacccttga tccgactcgt tcccttttgg gacggctggc tgtcgaaggg ctctggttca
     7621 gcgaggccct aggattagcg ccacccacgc gcgagcaacg agccacattt gttttggaga
     7681 tccaatctca cacacaatca ggagaaccca tgagagacac gtccgcaacc accataccca
     7741 cacagcctaa tgacgcattc gcggaattgg cttcttttaa gtcacatgtg gaggaactcg
     7801 aacgacaagc gtaccaaccc acccaaacct ctgcgcgtgc actgctagcg aaacaaaatc
     7861 cgcaagggtt ttggcagggc gacttaaccg ccgacacaac attggaatcc gactacgtgt
     7921 tgcttttact ctggctctat ccaccagaga acggtgtgtg ggagccaaga attcagacca
     7981 aagtcaagga ggccatacgc accatccttg accggcaact ttcagatggt ggatggaata
     8041 tttatacgga aggaccggcc gaagtaaacg ccacgacccg tgcatacgtg gcgcttcgag
     8101 ttgggggcta tgatcccgac caaccactta tgcagcgtgc acgtgaacgc attcttgccc
     8161 tcggaggcct tcaagccacc aatagctaca cgaagaataa cctgagcatg tttggtctct
     8221 tcccgagaaa gtacaccccc agtgttccac ctgaacttgt cctcatccct ggaaatgtct
     8281 tgtctgaaat ttcatcgtgg agtcgggcca tcgtcgtccc cctctccatt cttcaagcgt
     8341 ccggaataca acgcaaagtg cccggggatt ggaccgttga cgaactcatg gtgcccaatc
     8401 gaaaactgag gttcccgaga aaagaccctt tttctctcat gtttcatcag gtggacaaga
     8461 tcttgaaact ctgggaaaag cggggcttta aggatgttcg cgccaaggcc atacgcgaag
     8521 cagagaagtg gatgctcgat cacatgcgat tcacggatgg attgggagcg atttttccgg
     8581 gaatgatgta cgcccttatg gcgatggatg ctctgggcta tgagcgggac cacccggact
     8641 tcattgacac acttggccag cttgaagggc tcattcttga gaaagaagat gctctgcagt
     8701 ttcagccaag cttgtcccct gtatgggata ccgctatctc aatgtttgca ttaggagaac
     8761 tcggtgtcgg tgaaccgcaa gccatgcagc gggcagccga ctggttattg agcaaagaga
     8821 ttcgccgcaa aggagactgg tcagtcaagc gaccggattt gcagcctgga ggatggcctt
     8881 tccaatttgc gaatgaactc tacccggaca tcgacgatac cgccatggtc ttgttggccc
     8941 tacagcatgc caaggcatct gatcccgacc ggcagacccg cgccgaaggt cgtgccatca
     9001 actggttatt aggaatgcaa tcctcagacg gaggttgggc tgcctttgat gtcgataata
     9061 attgggccct actgaataag gtcccattcg cagaccacaa tgccatgctt gatcccagct
     9121 gccctgatat aacggggcgg gtgttggagg cactttgccg gcgtgattac acataccaaa
     9181 acccggcaat cgcgcgtgct gtccaatttc ttttgggcca tcaacaaccc aacgggagtt
     9241 gggatggacg ctggggcgta aattatacct atggaacatt cctcgccgtg cgtggccttc
     9301 gcgcaagtgg ctccccgact gcaaacgccg ccatccagcg tgctgccacc tgggtgcgct
     9361 caattcaaaa ccaggatggc ggttgggggg aaagctgtgc tagctatgaa aaacaggaat
     9421 ttgttccagc ctcaagcaca ccttcacaaa cagcctgggc cttacttgca cttgaagcgg
     9481 ctggcgatcg aacatcaaaa cccgttcatc gaggaatcga ctggctgctc actcatcaga
     9541 atcagcaagg agggtgggac gaggaactca tgacaggaac cggatttcct aacgttttct
     9601 atttgaaata ccatttgtac tcgcactact ttccattatt ggcccttgcc acttggcaag
     9661 caggattaaa gaactcctga ccatgctcta cgcttagaaa aaacgaatcc cctcttgaaa
     9721 ttttacaata atcttagaaa tttccgcttt tctaagaaat aaaacaggca ctcaaggaaa
     9781 ttattctgcg gcctatttct aagaacaaga gacaatgggc aaaatagtcc tccccgacag
     9841 acctttcgac tttggtcctg gcaggttttt ctgaatcccg ctatcgctgg ctttcaggct
     9901 tccagctcag gccgatattc agaattcgtt gaatcagcgc ttcataggaa ataccggctc
     9961 gttcagccga ctcggcgaga tcttcgccat acgccagttg agcattggga ttggcttcca
    10021 atatatacag gtgctcctgg ggatccaacc gaaaatccat tcgggcatag ccgtttaatc
    10081 ccaaaatatg aaaaacccgt ttggccgttt gttgaatatg cctcaccagt ttaggatgta
    10141 acccctcgac ctcgcccgat tgaatcccat attttttttg atatttcgtg ctccatttca
    10201 cccgggccgt agcaatcggc agagcatcag gaggaagatt tttcaagtgc agctcccaaa
    10261 ccggcagggc ttgcaatcgt cgattaccta acacacccac atagagttcc cgtccttcaa
    10321 tgtaccgctc gacaatggcg ctcgtgccca cactctgatg cacaaattca acacgttctt
    10381 tgagtttgtc ttcgtcatgg acgatagatg cttgagaaat gccgagggac gcttcctcac
    10441 taatggattt aacaatgacc gggaagggaa actcttctgc ccacttgatc actcgcccca
    10501 gtggatagac cacaaactcc ggacatcgga tgccatggta ggccataatt ttttttgacc
    10561 agcctttatc gcgagtgagc acgagcccct tgggattaca ccctgtataa ggaacaccca
    10621 ggagctccaa ataggacaca acattttgat cgaactccgg attgccattg aattcttcca
    10681 gcagattgaa agcaatgtgc ggcctccaat ccgttaccag cttatcaata acgcttaaat
    10741 catctctgac accgacggcc ataacctcat ggccaagatc ccgtagagtg gagaccacat
    10801 caaattcggt tttccattca acctcagcca aatcggcatg ctccacatcg tcggggggca
    10861 cgagatcctg gtgcataagg gccatgacgc gaagcttcct cataacacca cccgatgtcg
    10921 tccaccatgc aaatagttca tcgtttgcac cgtcaataaa atcgtaaaat ccacttttgc
    10981 ctgctcttca ggaacagcta accgcaaatt ccgttcacgg caatgtgcca tcatatcttg
    11041 taaaacttga tcaatggtat attgatactc tcccgtccat gccgcgactt tccggcgaac
    11101 ttcttttctg aatttcctga taaaattctc cgcggacgga tttgcggatt gatccggatc
    11161 cccggaaaag agacgccgca agtcccgatc ataaaagttt gggtagtcca gcccataccg
    11221 ttcccgcttg tcctgataat gttccctgag cgttttgtgc agtcgctgaa tagggtcgac
    11281 ctgttccctg gtcgtcacaa ttggcgcaac ccccgccaac tccttcataa gcccgtccat
    11341 atattccaat tttttaagtg ccggccatcc ggcataacgt tcacgccaat gggagtgagg
    11401 ggtgagccag acggcaaagg tttcagcaaa atcctcgtct ggatgactct gggcatacca
    11461 catatcaaga tgcaacacaa aacttttact atagggcttt ggcaaataac aatcgggata
    11521 cggaaccgaa gtatgcccga acatttgttg ccggctgcgc cgccgccgta agcggtaggc
    11581 attctcaatc gcatggcccg tttcgtgtcg aagaatccgc atacaccatt ccggggtccc
    11641 gccctccact tccaacattt gattaagttc cagcttcgct aaccgcggat gccccatata
    11701 aaacggcacg gcaagacccg gcgtgccatc aggcgaatac caatcgtcgg acaaccagac
    11761 atgggggcga aacactaacc cgtgatcttt caattcgcgg tataactgac ggatgcattg
    11821 acccaattca gttccagaaa tgcggatctt caaatcggac aaccggagat tcaacaactg
    11881 ttcatcagtc caggagaccc attcaggctc tgcggggaca gggggctgaa aatccggcaa
    11941 tagatccaaa ctcaccacct cccgtaccag tgtcggtttt gaaatatcat caaaaacact
    12001 ttcgtgagaa ttaggctatg ctcccttgac cgacccttcc gcctcaaccg ccaaacgatg
    12061 ctcaaatctt cactccattt cctctctaag tcaaaacctc gagcatgccc tcacctattc
    12121 tcatttgccc acttgaattc catagctttt acctttttgc ctatggtaga ttgcccctct
    12181 tttttaggat tccttcattc aggaccatgc aattttttat tcacactccg agtcagcgcg
    12241 tctgaagaat cagtgcccaa ggctttattt atttttttgg tcctcattca ttttctcggc
    12301 caaacgtctg tacgctttct tccttcttga ttctgacgac tcctgaagcc tgaccggtag
    12361 aaacacagga gattcatcta tgtatcgctg tgaaaaatgc cagggaacga tgttgctcga
    12421 tcgggaagtc gacatggaat caggaatgtc tcttctggtt ttctggtgta tcaattgtgg
    12481 cttacggaaa caagccgagc gagcaccgat tcctctcatc gaagtatcgt agcgcaagcg
    12541 catgattcct caatgttcca cccggattgg cagattaatc ctgagcgttc tgcctattcg
    12601 ggtcattgat gagcattttc agggaatcgg ggaaaaagag acagagaaga gatgccctgt
    12661 ctcatcgcgc accattcctg cgcgggcccg ggaatggcgt cggattcgct tcttgtgaga
    12721 gcatgaagtt ccgctttagg tagtcggtca ccgggaaaca aagcaatttg accccgatga
    12781 caaccatcag gaaccaggag agcaggagga actaggaatc cgcatccaat tccacattcc
    12841 aatataaata atcccgccaa ctttccggag cgtgacggat gccgatcatg agtgtcagcc
    12901 gtggagtcca ctttggcttg acgggctcct tgagtaattt catgcctgcc tcatggggaa
    12961 cacgcccgct tttccggtta ttacagcgcc aacatgccgt cacaatgttt tcccaggttt
    13021 tcttcccacc tttggcaata ggcaccacat gatcaaacgt caattcttca gtgcgaaacc
    13081 gtgcatagca atattggcag gtgtaccggt cacgggtaaa aatattaata cgagaaaatt
    13141 tcaccgcatg atgattcgaa cgaagcttga ccatgttgag aagacgcatc acggaaggca
    13201 atttaaagga aatggacacc cctcgaatat cccgatcata aaattcaagg acttcgactt
    13261 ttccttgcca taacaaattg atggcttttt gccagtgcac aactcgcaat ggctcatagg
    13321 tagcattgag taggagagtc atttccatac aaacgtactc ttgagacctc gccagattgg
    13381 aggaaactct gttcctatat caccgacgaa tccagagaat caagagagac catggggctc
    13441 ttgttgcgcc aggaataagt tgatatatta agggttttga ccgattagag atgaccatgt
    13501 ccttaaactt ccatccagtt gccaagattc aagatctccc tccaggcacc tgtcaaagca
    13561 ttgagctcca ggatcagggt attgccttat ttaatgtcga aggtgtgatc tatgcccttg
    13621 ataatacctg tcctcatgcc ggagggccac tgggcgaggg attcgtagag ggagatttcg
    13681 tcgaatgccc ctggcacgga tggaagtttg atatcaaaac cggggtttgc caaaaaaacc
    13741 cgagcccgag ctggaacgtg ccgtgctatg aagtacaggt ggttgatggc atcatccacg
    13801 tcgcccaacc aacatctgag aataggcaat tgtagaatac ttaaaaatct ggatttttga
    13861 aaatgccctt cctaccaaca actttcactg cctaatcacc acaacccaga tcgcgtaaac
    13921 tccgagacat ccaggtagat ccagtgagcc gtttaaataa ggagaccgcc cctagcagac
    13981 tcttcttcga actggctcag aacaggtaat ttcagcatca tgcccgga
//
LOCUS       DATIAL010000015        12421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1078229, whole genome shotgun sequence.
ACCESSION   DATIAL010000015 DATIAL010000000
VERSION     DATIAL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078229"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VLA60_00510"
     CDS             complement(<1..469)
                     /locus_tag="VLA60_00510"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSF07860.1"
                     /translation="MTKWIRWWGLGAFVVFAAIVGCVWIFFVDGWVKGAIEGAGTKAV
                     GARVEIDAADLSLFPAGLSLTRLQVTNPKTPMTNAVEVARVTMSLDGLNLFRRKVIVE
                     EMTVEGVRLDTPRTTSGAVQPVSDVSPEEESGMFSLALPALEVPDVKSILEKED"
     gene            complement(469..987)
                     /locus_tag="VLA60_00515"
     CDS             complement(469..987)
                     /locus_tag="VLA60_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HSF07861.1"
                     /translation="MIKLLAKLLRVLNSETDPGQISLGLCFAMIVGLTPLLSVHNLFV
                     VLLVFILRVNLSAFLLGLALFTGIAYLLDPLFHLLGLAVLTAPSLADLWTSLYQSVWW
                     RLEHFNNSIVMGSLVFSLALFVPVLLLSNLLIRRYRQHVMVWVQKSRVMQIFKASKLY
                     RTYETLSSWRPG"
     gene            complement(1090..2706)
                     /locus_tag="VLA60_00520"
     CDS             complement(1090..2706)
                     /locus_tag="VLA60_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HSF07862.1"
                     /translation="MNKIEVLKREKDGLDIKEDIARYAELGWEAIPDEDIQRLKWYGL
                     FLRNPTPGYFMIRVRIPGGRTTSVQMRTLAHLANTFGNGVLDLTTRQQFQLRHMKIEH
                     VPEVFRQMEETGLCSLQTGMDNVRNIMTCPVAGLTALEQLDATDLVKRLTEELVGQRA
                     YSNLPRKFNMAITGCLDNCLHLETQDLALVPATVEEEGSAITGFNLLAGGKLGSGGYR
                     IATPLDVFVTPREVVAVTGAILRVFRNHGCRDSRTTARLAFLLDDWGEERFRLEVQRE
                     VGWELSRAGTDARKSSVNDHMGVYRQKQSGWNYVGLKIPVGRIQADDLHGIARLADIY
                     GTGELRLAANQAVIMPNVSDRMLGDLTEEPLLQRFVYNPTPVQKGLVSCVGSDYCNLA
                     VIESKSRAVETAKRIETMIGTEMKPITMHWSGCPAGCGNHLVADVGLLGKKIKVKGQV
                     VEAVDIFVGGRSGPDPKPAIKLLEDVPCDTLAEVLSGILPYHSREKMHRTKAKTISKR
                     SVSRRAIDPEKTTSGRNETAATLITSPSMN"
     gene            complement(2759..3649)
                     /locus_tag="VLA60_00525"
     CDS             complement(2759..3649)
                     /locus_tag="VLA60_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HSF07863.1"
                     /translation="MHTVDVERMVQLAERKVEYLRSNPIGYLVLSALAGIYLGFGICL
                     IFSVGAPFWAGGSAGLKLVMGVSFGIALTLVIFAGSELFTGNNMVCALGALAKAITMK
                     EAGLIFIWSFAGNLAGSLGLAWLVAYSGVVGKAPQSDLLLHVASVKMNLSMGELFIRG
                     ILCNWLVCLAVWSAARTTNDAAKIMLIFWCLFAFIGSGFEHSIANQSLLGIALFLPHG
                     PDVSWAGFFWNQMWVVSGNIVGGVVFMGGAYWIISPVRAWIKKTERVVEGAAPDSSRP
                     IHLPAEGKLLPQPLVVGRRN"
     gene            complement(4095..4910)
                     /locus_tag="VLA60_00530"
     CDS             complement(4095..4910)
                     /locus_tag="VLA60_00530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HSF07864.1"
                     /translation="MRSQILSSVDTASQTPTPLNPWWGAWSAFLIVGCFYVVQALGFQ
                     MVWFVGGFVDGVVGDIEGGRGSSRLYPYMEIWLLPLSLCVGTMGAIVASIQVATHRAT
                     PSMDAVWFWKLLGKSCDFGSLWRFSLLGLGLALGFVACTEYGALPSDDLPQPLFDAMI
                     SAPLPLQVGWALMFIGLFPVVEEVLFRGFLFTGLSQSWGPSMAGLITTMIFVGVHMPK
                     VLEYWPALLAVSLIGSLTVLIRIRTGTLAPGIALHSTYNGVLVTAAFFTHTTS"
     gene            complement(5291..5746)
                     /locus_tag="VLA60_00535"
     CDS             complement(5291..5746)
                     /locus_tag="VLA60_00535"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HSF07865.1"
                     /translation="MENDLYQIIYASAATRPIEEDELFVMLKHAREKNKQLGITGMLL
                     HCDGSFIQALEGPKDQVMNMLGTIRQDSRHSRIAVLFEGPIQSRSFSQWSMGFNRPSK
                     EKLARSEGYTPYLDLGDDAQAIKNYSSVALKLIGAFRELSDKKSSAMEE"
     gene            complement(6006..6905)
                     /locus_tag="VLA60_00540"
     CDS             complement(6006..6905)
                     /locus_tag="VLA60_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSF07866.1"
                     /translation="MPMYRIHRFSKLTGLSPHVIRSWERRYGLVTPIRGENRYRLYTD
                     EDVMLFRYLKSQVDQGMAIGELSEQGVEYLRDQAQRVFVDTYREPPPSERLVLELSQA
                     LEENDRAGFERKLNGALAVIPFEEAVHRFLLPLQEQVGQLWHDGRLGVGQEHYASNQI
                     KQKIFSAMNQFQTSDEGPVVVVACPMNEWHEVAAMTAAYICRARGCRVHYLGANLPIP
                     ELAAYCYRNRPTCVLLSVTIMGSSDEARALAQEVAGKIIPLSPVGVGGQGVQAHAEEF
                     LEENIIVFQDLNALDRFVFSLKH"
     gene            7373..8323
                     /locus_tag="VLA60_00545"
     CDS             7373..8323
                     /locus_tag="VLA60_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF07867.1"
                     /translation="MKKLYRTIGGTMLISLLLITTPAISASPENMVLVPRGEFVMGSN
                     DHQDEKPHNVVLDAYYIDTYEVSNKDYKEFMKTTGHPAPAYWDDPRLNKPEQPVVGVN
                     WYDGSAFCEWKGKRLPTEAEWERAAKGPDGHAHYPWGHQLDSSKANYGQTVGQTTPVD
                     SYPEGKSGYGAYNMAGNVFEWVSDWYDPGYFNVSPAMNPQGPATGLNFANQGPVKTLK
                     GGSWLAPASSLHTSHRFWNQPENNSYGVGLGFRCAQSASPSGIQMVQYDFMLSLISMG
                     QEKWEEALQSIEKALKADPENAEYLQTRTLIMDQISKGKK"
     gene            8394..9506
                     /locus_tag="VLA60_00550"
     CDS             8394..9506
                     /locus_tag="VLA60_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF07868.1"
                     /translation="MTNIQPGEVTNERLSRSQGRLPVAGVLLGGLLLTINPSSSFAQD
                     TAGVPERMAYVPFGPAIMGIDKDPAEASVTGESATLYQRRMSMPWSKQAFHDEGPAHW
                     VVLDGYFIDKFEVSNSDFAKFMKEKGHPGPAYWDDPRLNKPDQPVVGVNWFDAKAYCE
                     YKGKRLPTEAEWERAARGPEGFQYPWGNELDPAKANYGKNHESTLPVNSMPEAASPYG
                     LHHMAGNVFEWVEDWYDPDFYKKTPHPVNTQGPLKPIWIGGTGTYVDRLTVGAKRVIR
                     GGSWIAAESSITTTHRFWNHPSNNSYGVGLGFRCAKTASSAVNDKVRTATIDAMKSMG
                     QEKWQEAKTHLTTALELDPHNTELQQMQEIVQGRIQ"
     gene            9516..9932
                     /locus_tag="VLA60_00555"
     CDS             9516..9932
                     /locus_tag="VLA60_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07869.1"
                     /translation="MSVNQPKKRIGKNIRVASSYLVSILFLTFLPWMLGGTPVYGVPP
                     VITEEKEEARDLIYQSSQATEEAWEEFHAAAIGGTLASPAVQSSIERQLHEARALLME
                     ARKAERDNRFDSVKTLTQKIQQITQNIVQASREKKQ"
     gene            9929..10756
                     /locus_tag="VLA60_00560"
     CDS             9929..10756
                     /locus_tag="VLA60_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF07870.1"
                     /translation="MKMWYVIIISLTLTGTLRDATAVEGPIIEKDPVEMITIPAGPFI
                     RGSAPGEGRLDEQPRRKIYLNAFAIDKYEVSNGHYMKFLEETLHKPPLNVFAERPFKQ
                     EEGIADLPVVQVTWHDAVDYCFWAGKRLPTEAEWEKAARGNDGRLYPWGDETPTPQKA
                     NYEKDWEEKKTFVEVTALPEGQSPYGLRNLAGNVREWVQDWYDSEYYSSAPDKNPMGP
                     EAGILKVLRGGSWKSFDTDLRATSRGKGGFALKTHGIGFRCARDIGPGQTASPSNAQ"
     gene            10774..11364
                     /locus_tag="VLA60_00565"
     CDS             10774..11364
                     /locus_tag="VLA60_00565"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF07871.1"
                     /translation="MMKILQALCVTSAVILFGFPMPDIAESGEGSRSTKGIRPEIVAD
                     FIYAVIESDRTLYTTHIVDRMQETGTVVASEAWESRNALPLPAQMLLLSGSAVKEKGI
                     GLEYRLASLLPIYQKNGPATDFEAAGLAAVEREPAQPYTGYITRGEQTYFQAIYADRA
                     ISKACVNCHNTHLLSPKRDRKLGDVMGGIIISFPVN"
     gene            11464..12387
                     /locus_tag="VLA60_00570"
     CDS             11464..12387
                     /locus_tag="VLA60_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSF07872.1"
                     /translation="MLAIIVYTFWWIFLPLDSGFAYEEVSSFTGGNLKGTVFIKGTPV
                     SPRRFNLVLNADPYYCGRISDGKGWRISPITRLGPDHTLPGAVVYLENVPRGKPAPST
                     PGVVQAKDCMFLPYVSTAKAGQEFHFQNWDPVEHKLEIFLTSDTGGLHLFGKNLPPHP
                     DNRKSDFLSEGTTGTHRAGSEVTYSVDETGVVLFRCSYHEYMEAWSVLLPHPYVTMAG
                     KRGEFSITDIPPGTYNLVIWHPMGQTTTTIRILPERTLNVDVDVFSTSATVYSEDTHK
                     PDPFGIDLVGDAHIVPSVELQEWDAPLEVGR"
BASE COUNT         3330 a         3337 c         2944 g         2810 t
ORIGIN      
        1 gatcttcctt ttctaaaata cttttcacgt caggcacttc tagagccgga agcgcaagag
       61 aaaacattcc ggactcctct tcaggtgaca catctgagac cggctgaacg gcgccggatg
      121 tggttctggg agtgtccaat cgaacccctt ccacagtcat ttcctcaaca atgacttttc
      181 tccgaaacag gttcaatccg tctagactca tcgtcaccct ggccacttcc accgcattcg
      241 tcatgggagt cttgggattt gttacttgca atctcgttaa ggagagtcct gccggaaaca
      301 ggctgagatc ggccgcatct atttccaccc tggctcccac cgctttggtc cccgcccctt
      361 caattgcccc tttcacccat ccatccacaa aaaatatcca cacacaaccc acaatggccg
      421 caaacaccac aaacgcccca agcccccacc accggatcca tttcgtcatc agcctggcct
      481 ccatgacgaa agggtttcat aggtccgata caatttgctg gctttaaaaa tttgcataac
      541 cctgcttttt tggacccaaa ccatgacatg ttggcgatac cgccgaatca ggaggttcga
      601 aagcagaaga acagggacaa acaacgcaag ggaaaacacc aggctgccca tcacaataga
      661 attattgaaa tgctccaatc gccaccacac cgattgatag agtgaggtcc aaagatccgc
      721 gagggacgga gcggtcagaa ccgctaaccc aagcaggtgg aagagcggat caagcagata
      781 ggcaatcccc gtaaacagcg ccagccccaa tagaaaagcc gataaattca cgcgcagaat
      841 aaataccaac agaaccacaa agaggttgtg cacgctcaac agtggggtaa gtcctacaat
      901 catcgcaaaa cacaatccca gggagatctg gccaggatcg gtttctgaat ttagaacacg
      961 aaggagtttg gcgagaagct taatcatgtc cttcaaaagg caggtgatgt agacatacta
     1021 tcagaatagc tttgaaaccg tggtacgtct acctaataga tggcagagga tttccaaaaa
     1081 ggttggggct taattcatac tgggtgaggt aattaacgta gcagcggttt cgttccttcc
     1141 gctggttgtc ttttccggat caatggcccg ccgagatacg ctgcgcttac ttatggtttt
     1201 tgcctttgta cgatgcattt tctcccggga atggtaagga agaattccgc ttaagacctc
     1261 cgccagggta tcgcacggga catcctccaa taacttgatg gccggtttgg gatcggggcc
     1321 ggaacggcca ccgacaaaaa tatccacggc ttcgaccacc tgacctttga ctttaatctt
     1381 tttccctaac agaccgacat cggctacgag atgattgccg cagcctgccg gacatcccga
     1441 ccagtgcatg gtgatgggct tcatctccgt gccgatcatc gtttcaattc gtttggcggt
     1501 ttccacggcc cgacttttag attcgatcac ggcgagatta cagtagtcgc tccccacgca
     1561 actgaccagg cccttttgta ccggggtggg attgtaaaca aagcgttgta acaaaggctc
     1621 ctccgtcagg tctcccaaca tgcggtccga aacattgggc atgatgaccg cttgattggc
     1681 tgccaggcgg agttcccccg tgccgtagat gtcggccaga cgggctattc cgtgtaaatc
     1741 atccgcctga atacggccga cgggaatttt gagccccaca tagttccagc ctgactgctt
     1801 ttgccggtag acacccatat gatcgttgac tgacgacttc cgcgcatcgg tccccgctcg
     1861 tgacaactcc catcccactt cccgctgaac ttccagacga aaccgttctt cgccccagtc
     1921 atccaacaaa aaagccagtc tggcagtggt gcgggagtcc cggcatccat gattccgaaa
     1981 gacgcgaagg atggctccgg tcacagccac gacttcccgg ggagtcacga acacgtcgag
     2041 tggggtggca atgcggtacc cgccggatcc aagtttgccc ccggccaaca agttaaatcc
     2101 ggtgatcgcg ctgccctcct cctcgactgt cgcgggcacc aaagccagat cttgtgtttc
     2161 aagatgtaaa caattgtcga ggcagccggt aatcgccatg ttaaattttc ggggaagatt
     2221 cgaataggcc cgttgcccta ccagttcttc cgtaagccgc ttgactagat ccgtggcgtc
     2281 cagttgttcc agcgccgtca acccggccac tggacaggtc ataatgttgc ggacgttatc
     2341 catgccggtt tgcaaggagc acaagcccgt ctcttccatt tgacgaaaca cttccggtac
     2401 atgctcaatc ttcatatggc gtaactgaaa ctgctgacgg gttgtcagat ccaacacgcc
     2461 gtttccaaag gtattcgcga ggtgcgccaa tgtgcgcatc tggaccgacg tggttcttcc
     2521 tcctggaatc cgcacgcgaa tcatgaagta tcccggcgtg ggatttcgca aaaataatcc
     2581 ataccatttc agtcgttgaa tatcttcatc cgggatcgcc tcccacccta attccgcata
     2641 tcgggcaata tcctctttga tgtccaagcc gtctttttcc cgcttgagca cttcaatctt
     2701 attcatcaga atcccttccc attcctttca cgattccatc aaccatccgt ggtaattcct
     2761 aatttctccg accaacaact aggggctggg gcaataattt cccctccgct ggtagatgga
     2821 tcggccgtga cgagtcaggt gccgcacctt ccacgaccct ttctgttttc ttgatccacg
     2881 cacgcacagg actgatgatc caataagcac ctcccataaa cacgacccca ccgacgatat
     2941 ttccggatac cacccacatc tgattccaga aaaacccggc ccaggaaaca tccggaccat
     3001 gtggaagaaa taacgcgatt cccagtaacg attgattcgc aatgctgtgt tcaaacccgc
     3061 ttccgatgaa cgcgaagagg caccaaaaaa tcagcatgat cttggcggca tcattggtcg
     3121 tgcgggcagc cgaccataca gccaaacaca caagccagtt gcaaagaatc ccgcgaataa
     3181 acagttcccc catcgaaagg ttcattttga ccgatgccac gtgcagcagg agatcgcttt
     3241 gcggcgcctt tcctaccacc cctgaatacg cgaccaacca ggccaacccc aatgatccgg
     3301 cgagattgcc cgcaaacgac cagataaaaa tcagcccggc ctctttcatg gtgatggctt
     3361 tcgccagagc accaagggca cacaccatat tgtttccggt aaacaattcc gatcccgcaa
     3421 agatgacgag ggtgagcgca atgccaaatg acacgcccat cacgagtttc aacccggcag
     3481 aacctccggc ccagaatgga gctcccacac taaaaatcaa acaaatccca aacccgagat
     3541 aaatgccggc cagggcggat aagacgaggt acccgatggg attgcttcgg aggtactcca
     3601 ctttcctctc tgccagttga accattcgtt cgacatccac cgtatgcatg acgttctcct
     3661 taaccaggtt gttcagttat ccaacaaaaa aaacgccaac tgcctgagct aagatcggat
     3721 tcttccgcct tgcccgagca gtggcgcctt tgccaccttc cccaccgttc gctcttcgaa
     3781 tgatggagta tgaattctgt ttcttaccta atggctaaac cgttcgtttg ggaaaattat
     3841 cgattcaaaa tctcggttcc ctcgacccat cacccatttt tcgcattcga ctaaaataaa
     3901 aaaaaacgcc caatcccctt ttaggaattg gacgcccatt gtccaccaac atgtctttgt
     3961 accatgacca gcaacttcat tgttgctatt gaaataatta acagacatgt cagttcctgt
     4021 caatcagggc taaagaccta ttcaatcgat taaggaaact tgtcacgaca cagtcccccc
     4081 gaaaaagggc tgccctagga agtggtgtgc gtaaagaagg ccgcagtgac taagacgccg
     4141 ttataggtgc tatgaagagc gattcctggt gcaagtgttc cggttcgaat gcgtatcagg
     4201 acagtcagcg aaccaatcag cgaaacggcc aatagcgccg gccaatactc aagcactttt
     4261 ggcatatgaa ctccgacaaa gatcatcgtg gttattaaac cggccatact cggtccccag
     4321 gattgcgaca atccggtaaa cagaaatccg cgaaagagca cctcttctac aacaggaaac
     4381 aacccaataa acatcaaagc ccacccaacc tgtaacggaa ggggcgcact gatcatggca
     4441 tcaaagagcg gttgaggaag gtcgtccgag ggaagcgcac cgtattcagt gcatgcaaca
     4501 aatcctaaag ctaagcctaa ccccagcaat gaaaatcgcc atagactccc gaaatcacag
     4561 gatttcccaa gaagtttcca gaaccagact gcatccatgg atggggtcgc tcgatgtgtc
     4621 gccacttgta tcgaggccac tatcgctccc atcgtcccca cacataggga caggggaagt
     4681 aaccaaattt ccatatatgg atataacctg ctcgatcccc tcccgccttc tatatccccg
     4741 accacgccat cgacaaaccc acccacaaac caaaccatct gaaaaccaag ggcttgaacc
     4801 acataaaaac atccgacgat gaggaatgcc gaccatgccc cccaccaggg gttcaaaggt
     4861 gttggagttt gtgatgcagt atcaacagag gagaggatct ggcttcgcat actgttcttt
     4921 cgggcattgg ctcgatttct tgatgtctaa actagaccgt tcatgagaac agaggtcacc
     4981 caaacagccc acctcggctc tcacactctc cccgaacgcc aaaaaatttg cttcgagacc
     5041 cgctcccctt cttggatcaa actatggcat gggcatgaaa aacacccatt ccaattcggc
     5101 caaaatagac gccaacagct cacgaacctg ccgttgttaa atgaggagca acgacatcgt
     5161 tgcatgagaa ttattcgcac cagacgaagc agcacttgtg atggtgggtg ggaaacgtca
     5221 cttcacggga gtgttatcga aatggaaggg tttgtctctt tgccaaaaga agatgtagga
     5281 gatcctggag ctattcctcc atggctgacg attttttatc agatagttca cgaaacgctc
     5341 caattaactt tagagccacg ctggagtaat ttttaatggc ctgagcatca tcgcctagat
     5401 caaggtaggg cgtatacccc tcgctcctgg ccagtttttc cttggagggt ctgttgaatc
     5461 ccatggacca ttgggaaaaa ctcctgcttt ggatcggccc ttcaaataac accgcaatgc
     5521 ggctgtggcg ggaatcctgc cgaatggtac caagcatatt catcacctgg tccttgggtc
     5581 cttcaagggc ttgaataaac gacccatcgc aatgcagtaa catgcccgtg attccaagtt
     5641 gcttgttttt ttctcgtgcg tgtttcaaca tcacaaacaa ttcatcttct tcaattggcc
     5701 gtgttgctgc actggcatag atgatttgat acaagtcgtt ttccatgaga cccagcctcc
     5761 ctcaactcgt caaattatct caggccacgt gctcttcatt tgaaagtgaa agtctgctca
     5821 acatgacgtt cgaaggccat cgcctctgaa tgatgggaaa aagcaccata tcagagcttt
     5881 caaaaatatg aaacagggaa tataggaagg ggagaagctt tcaaaaccga atattggaag
     5941 catgatcgga aatggattag attatataaa ttttctattg ggaaattggc ggggctatac
     6001 aggagtcagt gtttgaggga aaacacaaaa cgatcaagag cattcaagtc ctgaaagacg
     6061 atgatatttt cctccaaaaa ttcttcagca tgcgcctgga ctccttgtcc ccctaccccc
     6121 actggagaca gagggataat cttaccagcg acttcttgtg ccaaggctcg ggcttcatcc
     6181 gaagacccca tgattgttac ggaaagcaac acacaggtcg gacggttccg gtagcaatag
     6241 gcagccaatt cgggaatggg taaattggca cccaaataat gcactcgaca accccgtgcc
     6301 cggcagatat aggccgctgt cattgctgcg acttcatgcc attcattcat aggacaggca
     6361 acgaccacca ccggcccttc atccgatgtt tgaaattgat tcatggccga aaaaatcttt
     6421 tgtttaattt gattggaggc ataatgttct tggccgaccc caagccttcc atcgtgccac
     6481 aattgaccca cctgttcttg caatggaagc aaaaagcgat gcaccgcctc ctcaaaaggg
     6541 atcacggcta aggcgccatt caatttccgt tcaaaacccg cccgatcatt ctcttctaat
     6601 gcctgagata actcgagaac taacctttct gaggggggtg gctcacggta agtgtccaca
     6661 aacacccgct gtgcctggtc acgtagatac tccactccct gttcagacaa ttcaccaatt
     6721 gccatcccct ggtccacctg agacttgaga tatcgaaaca acatcacatc ctcatccgta
     6781 tacaggcgat agcgattttc tcctcgtatg ggggtcacca aaccgtagcg ccgctcccag
     6841 gaacgtatga catgaggaga gaggccggta agctttgaaa aacgatgaat tcgatacatt
     6901 ggcattaggt atttatatca atatgtataa tgtatgtcaa attttattaa acaaaaacta
     6961 ttgacaaaca ttaaacacaa tgttataact ttgaacatac attagacaaa cattaattat
     7021 taaaaaatta tttaccttta agccggagga actacaatga cactcaccaa accacagtca
     7081 atccagcaaa cgggaaggag agataatgag agcgctggaa aactttcaac tcactgttcg
     7141 cgttgcgggg gattcttggt gagtagtttt tgtatctcac ctgaccaggg aacctctgaa
     7201 tttcagattc ctgtgtggag atgtttgcaa tgcggagatc tcttcgatgc cacaatccta
     7261 aggaaccggc atcttgttca ccatcaatcc aacaactaac cggagggttc acgccatgaa
     7321 tgcaccacgg attgcctcaa tcactcattc accggaccag ggaaattttt ctatgaagaa
     7381 actttatcgg accataggcg gcaccatgct tatctcactt ctcctcatca cgacccctgc
     7441 cataagcgca agcccagaaa acatggtgct cgtacccagg ggagaatttg ttatggggag
     7501 caacgaccat caagatgaga aacctcataa tgtggtcctc gacgcctatt acatcgatac
     7561 gtatgaggtg tccaacaaag attataagga attcatgaag accacggggc atcccgctcc
     7621 tgcctattgg gatgatcctc ggttaaataa gcccgagcaa ccggtggtgg gcgtcaactg
     7681 gtatgacggc agcgcctttt gcgaatggaa ggggaaacgg ttaccgacag aggcggaatg
     7741 ggagcgagcg gcgaaaggtc ctgatgggca tgcccattac ccctggggac accaactaga
     7801 ttcttccaag gccaattatg gacagacggt tgggcaaacc actcctgtgg attcctaccc
     7861 ggaagggaag agcggctacg gagcctataa catggcgggc aacgtctttg aatgggtttc
     7921 agattggtat gaccccgggt atttcaatgt cagtcccgcc atgaatcccc agggtccggc
     7981 cacagggctc aactttgcga atcagggacc ggtcaaaaca ttaaaaggtg gatcctggct
     8041 ggctccggca agttcacttc acacaagcca ccgtttctgg aatcaaccgg agaacaattc
     8101 ctatggcgta ggcttaggtt ttcgatgtgc tcagtcggct tcaccatcag gcatccaaat
     8161 ggtccaatat gatttcatgc tctctctgat cagtatgggt caggaaaagt gggaagaagc
     8221 tctgcagtct attgaaaaag ccctgaaggc cgatccggaa aatgcggaat acctgcagac
     8281 ccggactttg atcatggatc aaataagtaa agggaaaaag tgagtcgggt aagtcttgct
     8341 cgacatattc taaacattca tccataagcc tttaaccaaa cggtaagaac gtgatgacaa
     8401 acatacaacc aggagaagtc acaaacgaac gcttgtccag atcacaagga cgcctgcctg
     8461 tggcaggcgt cctcctgggt gggctcctgt taacgattaa tccgtcgtcc tccttcgctc
     8521 aggatactgc cggagtgccc gaaagaatgg cctatgtgcc cttcgggcct gccatcatgg
     8581 gaatcgataa agacccggca gaggccagcg tcacgggtga atccgccacc ctgtatcaac
     8641 gacgaatgag tatgccctgg tccaaacagg cctttcatga tgagggaccg gcccactggg
     8701 tagtccttga tgggtatttc attgataaat ttgaggtatc caacagcgat tttgcaaaat
     8761 ttatgaagga gaaggggcac cctggaccgg cctattggga cgaccctcga ttgaataaac
     8821 ccgaccagcc ggtggttggt gtgaattggt ttgacgccaa ggcctattgc gaatacaagg
     8881 gaaagagact cccgaccgaa gccgaatggg aacgggccgc tcgaggccca gagggatttc
     8941 aatatccgtg gggaaatgag ttggatccgg caaaagccaa ctatggcaaa aatcatgaat
     9001 ccaccctccc agtgaattcc atgcctgaag cagcaagccc ctatggactc caccacatgg
     9061 caggcaacgt ctttgaatgg gtggaagact ggtatgaccc ggacttttac aagaagacac
     9121 cgcatcccgt gaacacccag ggtccgctta aacccatctg gatcggagga accggcacat
     9181 atgtggatcg cctaaccgtc ggagccaagc gggtcattcg gggtggatca tggattgccg
     9241 cagaatccag tattaccacc acgcatcggt tctggaacca tccttccaac aacagctatg
     9301 gagtgggtct tggattccga tgcgctaaga ccgcttccag tgcagtgaat gacaaggtac
     9361 gaacggccac cattgacgcc atgaagagta tgggacaaga aaaatggcag gaggccaaga
     9421 cgcacctgac gactgccctt gaacttgacc cccataatac agaacttcaa cagatgcagg
     9481 agatagtcca agggagaatt cagtaggaac agtccatgag tgtcaatcaa ccaaagaaga
     9541 gaataggaaa aaacatccga gtggcctcct cctatctggt aagtatcctc ttcctcacct
     9601 tccttccatg gatgcttggt ggaactccgg tctacggagt tccacccgtg attacagagg
     9661 agaaggaaga ggcccgggac ctcatttatc aatcctccca ggccaccgaa gaagcctggg
     9721 aggagtttca tgctgctgcc atcggcggaa cactggcttc accagcggtt cagtccagca
     9781 ttgaacgaca gttacacgaa gcccgcgcgt tattaatgga ggccagaaaa gctgagcggg
     9841 acaacaggtt tgattcagta aaaactctca cgcaaaagat acagcagatc actcaaaaca
     9901 ttgtgcaagc cagtcgggag aagaaacaat gaagatgtgg tatgtcatca taatctcact
     9961 cacgctgaca ggcaccctac gggatgccac ggcagtggaa gggcccatca ttgaaaagga
    10021 cccggtagaa atgatcacca ttccggccgg cccattcatt cgcgggagcg cccccggaga
    10081 gggtcgcctc gacgaacaac ctcgccgcaa gatttatctc aatgcctttg ccatcgacaa
    10141 gtatgaagtg agtaatgggc actatatgaa attcctggag gaaacattac ataagccccc
    10201 actgaatgtg tttgccgagc gcccgttcaa gcaagaggag gggattgccg accttccggt
    10261 tgttcaggtc acctggcatg atgccgtcga ttattgcttc tgggccggca aacgtcttcc
    10321 caccgaggct gaatgggaaa aggcggctcg aggaaacgat ggccgtctct acccctgggg
    10381 agacgagact cccactcccc aaaaagcgaa ctatgaaaaa gactgggaag agaaaaaaac
    10441 ctttgtggag gtgacggctc ttccggaagg ccaatcacca tacggacttc gaaacctggc
    10501 cggcaatgtc cgtgagtggg ttcaggattg gtacgactcc gaatactatt ccagtgctcc
    10561 agacaaaaat cccatgggcc ccgaagccgg gattctcaaa gtgctccgtg gaggttcatg
    10621 gaaaagtttt gataccgatt tgagagcgac ctccagaggc aagggtggat ttgcattaaa
    10681 aactcatggc attgggtttc gatgtgcgcg ggacatcggc ccaggtcaaa ccgcgtcacc
    10741 aagcaatgcc cagtaggggg ccatgaaggt gtcatgatga aaattctaca agccctttgt
    10801 gttacttcag cggtgatcct gtttggcttt cccatgccag atatcgccga gagtggagaa
    10861 ggcagccggt cgacaaaggg aatcagaccg gaaatcgtgg ccgatttcat ttatgcggtg
    10921 attgaatcgg atcgcacctt gtataccacg catatcgtcg accgcatgca ggaaacaggt
    10981 accgttgtcg cctcggaagc ctgggagtcc aggaacgctc taccccttcc ggctcagatg
    11041 ttgctgttat caggatcagc agtgaaggag aaaggcatcg gccttgagta tcgtctggca
    11101 agccttcttc ccatttatca aaaaaatggt cctgccactg acttcgaagc agctggtctg
    11161 gcggccgtag aacgtgaacc tgctcaacca tacacaggct acatcaccag gggggagcag
    11221 acatatttcc aggcaattta tgcagaccgg gcaatttcca aggcctgcgt caattgtcat
    11281 aatacgcatc tgctcagtcc taagcgtgat cgcaagctcg gtgatgtcat gggaggaatc
    11341 atcatctcct tcccagtgaa ttaagtgagc cagcctagaa caaaaatcac taggcacgat
    11401 ggcgacaggc agacaaccat gctgaatgtg agaacagagg ccttccctac cagaagagga
    11461 acaatgctcg caattatcgt ctacactttc tggtggatct tccttcccct ggattccgga
    11521 tttgcctatg aggaagtctc aagttttacc ggcgggaatc tgaaagggac tgtattcatc
    11581 aagggaacgc ccgtttctcc tcgacgcttc aatctcgttc tcaatgccga tccctattat
    11641 tgtggacgca tttcggatgg gaaaggatgg cgtatttcac ctatcactcg attgggacca
    11701 gaccacactc tcccgggtgc cgttgtctat ttggagaatg ttccacgggg aaaacccgct
    11761 ccctcgacac cgggcgttgt acaagccaaa gactgcatgt ttcttccgta tgtcagcacc
    11821 gcaaaagccg gtcaggaatt tcattttcaa aattgggatc ccgttgaaca caagctcgaa
    11881 atttttctga cctctgacac gggtggactt catctgttcg ggaagaatct tccaccgcat
    11941 cccgataatc gtaagagtga ttttctttca gaaggcacca ccggcacaca tcgagccgga
    12001 tcggaagtca cttattcggt ggatgagacg ggggttgtcc tctttcgctg tagctaccat
    12061 gaatatatgg aagcttggag tgtccttctt ccacatccct atgtcactat ggccggaaaa
    12121 cgaggtgaat tttccatcac cgacattccg cctgggacct acaatctcgt catctggcac
    12181 ccgatgggtc agaccaccac gaccattcgc atccttcccg agcgcactct caacgttgac
    12241 gtggatgttt tctcaacctc ggccaccgtc tattctgaag acacccataa gcccgatcct
    12301 tttggcatcg atctggtagg ggatgctcat attgttcctt cagtggaatt acaagaatgg
    12361 gatgccccct tggaagtcgg aagataggag actggacatg aagattgtca ttgccggtgg
    12421 a
//
LOCUS       DATIAL010000016         2227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1400818, whole genome shotgun sequence.
ACCESSION   DATIAL010000016 DATIAL010000000
VERSION     DATIAL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400818"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VLA60_00575"
     CDS             <1..1430
                     /locus_tag="VLA60_00575"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HSF07873.1"
                     /translation="VLFLGFQDLNYHIKFLGIGKGKLMKQILVVGAGSVGGYFGAHLA
                     RHNPNVAFLLRPLTMAAVAKNGLTIRSAIGESFTVHPPVASDPRELPQPDLIVLGVKA
                     YDLEKVMSQLEPVLKPDTTILTLQNGVTIEDALMARFGRERVVGGVAFIYSRIVEPGV
                     IDHYKKGMMTIGEPMGMESPRLQAIAALWKEAGIPCFITRDIRRAKWEKMCWNCVFNP
                     LTVVLNDRVAKALDHPELQPVITTIVREVSAVAMAAHRVPLDEDMPEKVVKWSQELRD
                     IHTSMYDDWKAGRPTEIDDLNGYITMKGKEFGVPTPMNEMLTALIKAMSGPQIPAEAS
                     IRIEGAIMQPLQFTRTTLAQLPVVHQVSELPTLMPGTNVRGIKLQALLDIVTLHVGAD
                     HVTFESQDGKFSACLTTRQAADFGILVYEQDGAPFPDSRGGPFRLVTPGLGDLCANVK
                     QVGRIIFSQGVLADTRPPQGCAEER"
     gene            1437..>2227
                     /locus_tag="VLA60_00580"
     CDS             1437..>2227
                     /locus_tag="VLA60_00580"
                     /inference="COORDINATES: protein motif:HMM:NF026052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07874.1"
                     /translation="MESSRHVPLIYNLFPRLAGPCNRWIAHAERAADMGFNWLFVNPV
                     HLPGFSGSLYAIKEYDLLNPAFLPEGTHDQRLDVLRPTFQSIAKTGLAPMVDLVLNHT
                     AIDSSLVVEHPDWYVRNAQGQVQNPYAVDPDDPSKKTVWGDLAEIDNQDSPDREHLWE
                     YWGQLVERYLELGVHGFRCDAAYKVPAELWRFLIDRALRVNPQVVFWAENLGCTLEQT
                     HALGDAGFHLFCNSSKWWNFKDVWCLNQHREFQNMPSVAFPETHDT"
BASE COUNT          494 a          520 c          653 g          560 t
ORIGIN      
        1 aggtcctatt cctgggtttt caagatctta actatcacat taagttttta ggaattggga
       61 aaggtaagct gatgaagcag attttagttg tgggggcggg gtcggtcggc ggatattttg
      121 gcgcacatct ggctcgccac aatccgaacg tggcgtttct cctacgaccg cttacaatgg
      181 cggctgttgc caaaaacggg ctgactatca gaagcgcgat tggtgagagt tttactgttc
      241 atccgcccgt ggcttccgat ccaagggaat tgccacaacc ggatctgatt gtgctgggag
      301 tcaaagccta tgacctcgaa aaggttatgt cccagcttga gccggtgctg aaaccggaca
      361 ccactattct gacgttgcaa aatggagtga ccattgagga tgcgctgatg gcccgttttg
      421 gccgggaacg cgttgtgggt ggggtggcct tcatttattc caggatcgtg gaaccgggcg
      481 tgatcgatca ttataaaaaa ggcatgatga ctatcgggga accgatggga atggaatccc
      541 ccaggcttca agccattgcg gcgttgtgga aagaggcggg cattccctgt tttatcacta
      601 gagatatccg gcgcgcgaag tgggagaaga tgtgttggaa ttgcgtcttt aatccgctga
      661 cggtggtgct gaacgatcgg gtggcgaaag ccctggatca tccggagttg caaccggtca
      721 tcacgaccat cgtgcgggaa gtctcggccg tggccatggc ggcacaccgt gttccattgg
      781 atgaggacat gccggagaaa gtcgtgaagt ggtcgcaaga acttcgggat attcatacct
      841 caatgtatga tgattggaaa gccggacggc ccaccgagat tgatgacctg aatggctata
      901 tcacgatgaa ggggaaggag ttcggggtgc cgactcccat gaacgagatg ttgacggcct
      961 tgatcaaggc aatgagcggg ccgcaaattc ctgcggaagc ttccatccgg atcgagggag
     1021 cgatcatgca accgctccaa tttacgcgaa cgaccctggc ccaacttcca gtcgtacatc
     1081 aagtgtctga attgcctacg ttgatgccgg gcacgaacgt gcgtggaata aaacttcaag
     1141 cgttacttga tattgtgacg cttcatgtgg gagccgacca tgtgaccttc gagtcgcagg
     1201 atggaaaatt ttcggcctgt ctgacgacca gacaggctgc tgattttggc attctggtgt
     1261 atgagcagga tggtgcgccg tttcctgatt cgcgcggcgg gccgttccgg ttggtgacac
     1321 ccggattggg cgacttgtgt gccaatgtga agcaggtcgg ccggatcatt ttttcccaag
     1381 gggtgcttgc ggatactcgt ccgccacagg gctgtgcaga ggaaagatag gggtccatgg
     1441 agtcttcgcg acacgttcca ctcatctaca atttgtttcc ccgcctggcg ggtccctgta
     1501 atcgatggat tgctcatgca gaacgggctg cagacatggg gtttaattgg ctttttgtga
     1561 accctgttca ccttccgggt ttttcgggaa gtttgtatgc gattaaagaa tatgatctcc
     1621 tgaatccggc atttcttcca gaaggaactc acgatcagcg tctggatgtc ttgcgcccta
     1681 cgtttcagtc catagcgaaa accggcctgg ctcccatggt ggatctcgtg ctgaaccaca
     1741 ccgcgatcga ttcctcttta gttgtggagc accccgactg gtacgtgcgt aatgctcaag
     1801 gacaggttca aaatccttat gctgtggatc ccgatgatcc aagcaagaaa accgtatggg
     1861 gcgatttagc ggaaatagat aatcaagatt cccctgaccg cgaacatctc tgggagtact
     1921 gggggcaatt ggtcgagcgg tatctcgaat tgggagttca cgggttccgg tgtgacgcgg
     1981 catataaagt tcccgctgag ctgtggcgtt ttctcattga ccgggctctc cgtgtcaatc
     2041 ctcaggttgt tttttgggcg gaaaatcttg gctgtacgct ggagcaaacg cacgcgcttg
     2101 gggacgccgg atttcacttg ttttgtaata gttccaaatg gtggaatttc aaggatgtct
     2161 ggtgtttgaa ccaacatcgg gaatttcaaa atatgccgtc cgttgcgttt ccggaaacac
     2221 atgacac
//
LOCUS       DATIAL010000017         3171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_540779, whole genome shotgun sequence.
ACCESSION   DATIAL010000017 DATIAL010000000
VERSION     DATIAL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540779"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /gene="bioF"
                     /locus_tag="VLA60_00585"
     CDS             <1..1166
                     /gene="bioF"
                     /locus_tag="VLA60_00585"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HSF07875.1"
                     /translation="SMFKEDLQTLAQQHLLRELSLINSPSAPVISVEGREVLLFASNN
                     YLGLANHPQVKDAAIKAIGKFGVGSGASRLISGNVTPHQVLEGELASFKETEAALTFS
                     SGYATNIGVIPNLARADSLILADRLCHASLIDGCRLSRATLRVFHHNDVSHLKRLLAK
                     TRSRTSTLILTEGVFSMDGDVAPLPEIARLAEEYGATLLIDDAHGTGVMGATGRGTVE
                     HFGLDSRHILQMGTLSKALGSSGGFIAGPKDFVAYLVNTARSFIYTTAPPPAIAAAAS
                     AALATIRQEPQRRVTLWRNREFLHHRLVTMGFQLTDTQSPILPIMVNDPEIGVHMSQH
                     LRAEGVWIPAVRPPTVPKGTSRLRITVTADHSLEHLETALRALRKVGTALKVL"
     gene            1451..>3171
                     /locus_tag="VLA60_00590"
     CDS             1451..>3171
                     /locus_tag="VLA60_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HSF07876.1"
                     /translation="MKTQLLPGKIEIQGEIAKGQTGTIYYGYDLELRQEMAIKVYHTH
                     INGRLIRGKAFIEKAKSLLRLEHPNLIKIFKVEEQDGTPVVCMEFFDAPSLQHVIQNE
                     GPLSVEKMLLLAREIAEVLVHIHFQGIIHGTLHPGHVLVGPQGQIKVMDLGLSWILMD
                     ILKICDAELLRPLPYLLPETAKSELLTLGSDLYCLGFMMYDMLTTRVPYSGLPKTSIM
                     GKLAFDQADPVFDFPDHVPKAICELIRQMTRNQSSQRLQDATHVLTILNQQLAKFAPE
                     VLRPPAAPTEPQPTPDIQTAKIKTEAPSLPPQAPVSVPLPIQPPAVTRPKHSTDYQGI
                     HRARTRKKLGVTIGITLFLFIGGTMGYMYKEQLGIPFLASHQLGISEQPLINEPAPVV
                     PPIDSFTTIPPDNPISSPLNTPTPEVPHVKTNDTPNSDNSNRAIPTPPTPTTVVEDHS
                     LKPDASHHLETPLPSPLERTSSQSLNPSPQHTSEGTQVPDQPNSSPSQGHSNMDNPPS
                     SKKPVTPVTTPASTTNSPATGQRTVPTVTEKPASQSEQSSALSPKGPQSLQDSTAPSP
                     NTESLDN"
BASE COUNT          837 a          943 c          692 g          699 t
ORIGIN      
        1 tctcaatgtt caaagaagac cttcagacct tagcgcaaca acacctgttg agggagttat
       61 ctctcatcaa ctccccctca gctccggtta tttcagtgga agggcgggaa gtcctccttt
      121 ttgcctccaa taactatctt ggtctggcca accatccaca ggtaaaagat gccgccataa
      181 aggccatcgg aaaatttgga gtaggatctg gagcctcacg gttaatttcc ggcaacgtca
      241 cgcctcatca agttctagag ggtgaattgg ccagttttaa ggaaacagag gcggctctca
      301 ccttttcttc aggttacgcc accaatatag gagtcatccc gaatcttgcc agggcagata
      361 gcctgatttt ggcggatcgg ttatgccatg ccagtctgat cgatggttgc cgcctcagcc
      421 gggccacatt acgcgtcttc catcacaacg acgtcagcca tctcaagcgc cttctggcaa
      481 aaacacgctc ccggacttcg acgctcattc tgactgaagg ggtgtttagt atggatggag
      541 atgtggctcc gctacccgag atagccagat tggcagaaga gtatggtgcc accctgctca
      601 ttgatgacgc gcatggcacc ggggtcatgg gtgcaaccgg tcgaggcacg gtggaacatt
      661 ttggattaga ctcccgccac atcctacaga tgggcacgct cagtaaagcc cttggatcca
      721 gtggcgggtt tatcgccggg ccgaaagatt tcgtggccta tctggtgaat accgcacgct
      781 catttatcta taccaccgcc ccaccgcccg ctattgccgc cgcagcgagc gcagccctgg
      841 caaccatccg gcaagaacct caacgcagag tgacgctttg gcgcaatcga gagtttttgc
      901 atcaccgctt agtgaccatg ggattccaac tgaccgacac tcagagccct attctcccga
      961 taatggtgaa cgacccggaa atcggggtcc atatgagcca acacttacgg gcagagggcg
     1021 tgtggattcc ggctgtccgc ccccctacag tccctaaagg gaccagccgc ctccgcatca
     1081 cggttacagc cgatcattcg cttgagcatc tcgagacagc cctcagagca ttacggaagg
     1141 taggaacagc ccttaaggtt ctctgagcga aggattgccg ccctctgctc ctcttatgcc
     1201 tgccagccga tcctgagaaa cgccctctct ttacgtcctg cattcgaaat ttccaagaga
     1261 tcaagaatcc cttccattca agaaaatcaa accgtattcc gacaagatga gcaggaattc
     1321 catggctccg agatttccgc attgattgat tgattgattg attgataggg gtgccttcgt
     1381 ttcaatgcac cacgaccacc agacgcactc attcaccaca aagcttattt taaagagtta
     1441 ggttttttgt atgaaaacac aactgctgcc tggaaaaatt gaaatacagg gagaaattgc
     1501 caaaggccaa accggaacca tttactatgg gtatgacctc gaactgcggc aggaaatggc
     1561 gattaaggtg tatcataccc atatcaatgg tcggctcatt cgtggaaaag cctttatcga
     1621 aaaagccaaa tcgctccttc gattagaaca ccccaatctc attaaaatct tcaaagtaga
     1681 agagcaggac ggcacccctg tcgtctgcat ggaatttttt gatgctccca gtctccagca
     1741 cgtcattcag aatgaagggc ctctttcagt cgaaaaaatg ctcttgctgg ctcgtgaaat
     1801 tgcggaagtg ttagttcata tccactttca aggcatcatt cacggcacac tccatcctgg
     1861 acacgtcctg gtcggccctc aaggccagat caaagtcatg gacctcggat tatcttggat
     1921 cctgatggac atactaaaaa tctgtgacgc tgaattactc agaccattac cctacttgct
     1981 cccggaaact gccaaaagcg aacttttgac cctcggcagt gatttgtact gtttgggatt
     2041 catgatgtac gacatgctca ccacgcgagt gccctattcc ggtcttccaa agacatcgat
     2101 tatggggaaa ctggctttcg atcaggctga cccggtgttt gacttcccgg accatgtccc
     2161 gaaagccatc tgtgagctta tccgccaaat gacccggaat cagtcctcac agcgacttca
     2221 agatgccacc catgtcctca ccattttgaa tcagcaattg gcaaaatttg cccctgaagt
     2281 tttgaggccg ccagcggccc ctacagaacc acaaccaacg ccagacatcc aaacagccaa
     2341 gatcaagaca gaggcaccga gccttccacc acaggcacca gtatcagtac cccttccaat
     2401 ccaaccgcct gccgtaactc ggccgaaaca ctctaccgac tatcaaggaa tacaccgagc
     2461 caggacgaga aaaaaattag gcgtcacgat tggaatcacc ctgtttctct ttattggtgg
     2521 aaccatgggt tatatgtata aggaacagct cggcatccct tttctggcat cccatcaact
     2581 cgggatctcc gagcagccgc tcatcaacga accggctcct gtcgttccac caatagactc
     2641 attcacgacc ataccccctg acaacccaat ttcttctccg ctcaataccc caacgccaga
     2701 agtgccacac gtgaaaacca acgacacacc aaatagtgat aactcaaacc gggcaatccc
     2761 gacaccccca acgccgacaa cggtggtgga ggatcactca ttgaaacctg atgcatcaca
     2821 ccacctggaa acaccattgc cttcgcctct ggaacgcacc agctcacaat ctctgaaccc
     2881 gagcccacaa catacatctg aaggcaccca ggttccggat caaccaaact cgtcgccatc
     2941 ccaaggccat tccaacatgg acaatccacc atcttccaaa aaaccggtta ctccggtcac
     3001 gacaccggcg tcgacaacaa attctccagc aacggggcaa agaactgtcc ccacagtcac
     3061 ggagaaaccc gcgtcacaat ctgaacagtc ttcagcgctt tctcccaagg gaccgcaatc
     3121 cttacaggac tcaactgctc cctccccgaa cacagagtcg ttagacaaca t
//
LOCUS       DATIAL010000018         4065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1401434, whole genome shotgun sequence.
ACCESSION   DATIAL010000018 DATIAL010000000
VERSION     DATIAL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401434"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            899..2911
                     /gene="hyfB"
                     /locus_tag="VLA60_00595"
     CDS             899..2911
                     /gene="hyfB"
                     /locus_tag="VLA60_00595"
                     /inference="COORDINATES: protein motif:HMM:NF005086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HSF07877.1"
                     /translation="MALLLAYACGMLLPLCFPSRPHIQNMIAHGSAAVAGGLGIILGL
                     IGLTAPEPLAWFLPSSIPLLTFSLRLDSLSAFFVLTISLVGLAVSVFASGYVREFYGR
                     IPVSLLGFLYNGFLLSMLLVVTADNAFFFLIVWELMSLLSYFLVVSEHEKPDVRFAGL
                     FYLIMTHVGTAFILVTFLMFYQETGSFAFEAFRSDDQSLPEGLRTLVFWTALIGFGTK
                     AGIVPLHVWLPYAHPAAPSHVSALMSGVMIKTAIYGLLRVYFDFLGGPFPWWWGFVVL
                     LVGTVSALLGVMYALMEHDLKSLLAFHSVENIGIILMGIGTGMVFYAYDMPHLAALGI
                     IAGLYHTLNHAVFKALLFMGAGSLLYATHTRNMEEYGGLLRRMPWTGLFFLIGAVSIS
                     ALPPTNGFVSEWLVFQGLFLSLELPSLFLKLMLPIAAALLALTGALALACFVKAFGIS
                     FLALPRSSQARKALEVPVPMRVGMGFLAGLCLVLGLGPAVVVPLLDRVSEPFVGISIA
                     GRVFEMGGWTLAPVSREFSSLSTPVLAIVLAGLTGLGWGLAYVLGGKGKTRFYKTWGC
                     GLNVTARMEYTATGFVQPIKRVFSTIYQPTVKLETEFLEESHYFAKRRHFEFFIEPIF
                     LKYLYHPILGFFSTLADRSRVIQGGNLSLYLAYIFVTLVFLLMVMG"
     gene            2921..3874
                     /locus_tag="VLA60_00600"
     CDS             2921..3874
                     /locus_tag="VLA60_00600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HSF07878.1"
                     /translation="MSAIDFLLIILQIFLVLGLSPLIVGVIRRVKAHLQCRHGANVFQ
                     PYADLAKLFRKEPVISTTASWIFPATPYILFSSTLSASLLVPTIIAPMPLNFAGNIIA
                     LVYLLALGTFFLMLAGLDAGSAFGGMGSSREAIVASLTEPALILSLFAVALTAGSANI
                     SAIVYKSAILQGIVLDPPPHLMALVALFIITLAETGRVPVDNPATHLELTMIHEAMIL
                     EYSGRYLALVEWAAGMKLMIFLTLLANIFAPWGIATTLSPSALGLGLLVFVGKVVGLA
                     VVVGVFECMFAKLRLFRVTELLGAAFILSLLALVFFYILRV"
     gene            3876..>4065
                     /locus_tag="VLA60_00605"
     CDS             3876..>4065
                     /locus_tag="VLA60_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07879.1"
                     /translation="MPTLPINLGSYLVNLCSALLLLTCFAIVAQRRLMACVDLFALQS
                     VFLAVTATLVAFLTGIHHI"
BASE COUNT          787 a          958 c         1048 g         1272 t
ORIGIN      
        1 gaagtccgcc ttgcgagcca taaattcaga aaagtcttta atctatagag aattttgata
       61 aataggcata aacataaatg tgcaaaattt gcatcatcac ttaggaaatt tttcaggaaa
      121 atagagtcta catggatcaa gggttcactt gtttttccct gggtatatgg aatgtgcacc
      181 acgtctgtcc tggctatttt gatagaaatg caatgcacgg gaagtgttcg attcatactg
      241 acgaaagcct tgttggaatc agggatcaat ctgaaagccc aatgcaaccc tattggtctg
      301 ttgatcaggt tgaaaatggt cgtaggtcag ctcaatgaat catctggacc taaggcctgc
      361 aaggtttgca gacgtgatac tgaaatgttt tgtttcattg taattcctta accggcttcg
      421 agctatctta gggcgttttg ctggtgttag gcagaacgtt ggagcaagaa cagaatgaat
      481 tttcaaggta tagcttttgc acagaaatgg atctgtgcat gaaagccaca acaccataag
      541 agaatttgag ggtacggtta agcagatacg tctcagaatg gcgatcttct cctcaggcga
      601 tcagtatttt gcttctttcg cctggccatt ttcccaatcc ccaataattt tacgcacagg
      661 tcacatattc aaggaaacca gaactccttt gcccccacca agcctgctca cgaggaaatc
      721 cggtgttttc tcctctattc acgtgtctct ttatgcgatg gccatgaaac gttcttatga
      781 ccagcatttg atattcctgt tctctcaatc aggcgaggcc aattgccttg acacctcctc
      841 ttgccggttt ggcaaatgaa agggacaccg taagcaaggc atgaggatat tcctgttctt
      901 ggctctgctg ttggcctatg cgtgtgggat gcttctcccg ctgtgtttcc caagtcgacc
      961 gcatattcag aatatgattg cgcatggctc agctgccgtg gccggcggcc tgggaataat
     1021 ccttggtctg atcgggctca cggcccctga accgctggct tggttcctgc cgtcttctat
     1081 tcctcttctg acgttttctc ttcggctcga ttctctgagt gctttttttg tcttgacgat
     1141 ttcccttgtg ggattggcgg tatccgtgtt tgccagtggc tacgtccggg aattttacgg
     1201 acgaattcct gtcagtcttc tcggctttct ctataacggg tttcttctgt ccatgttgtt
     1261 ggttgtcacg gcggataatg ctttcttctt tttaatcgtt tgggaattga tgtccctcct
     1321 ctcatatttt ctcgtggtat cggagcatga aaaaccggac gtccgttttg ccggattatt
     1381 ttatttaatt atgacccatg tgggtacggc atttattctg gtgacgtttc tcatgtttta
     1441 tcaggagacg ggttcgtttg cctttgaggc ctttcgaagc gacgaccaat ccctaccgga
     1501 agggttgcgg accctggttt tttggacagc gctcatcggt tttggaacca aagcgggcat
     1561 tgtgccactt catgtctggt taccctatgc ccatccggct gcaccgtctc atgtctccgc
     1621 cttgatgtcg ggtgtcatga ttaagacggc aatttatggc ctccttcgtg tgtattttga
     1681 ttttcttgga ggaccgtttc cctggtggtg gggatttgtg gtcttgttgg tgggcacggt
     1741 gtcggcgctt ctcggagtga tgtatgcatt gatggaacat gacctgaaga gtcttctcgc
     1801 cttccacagc gttgaaaata tcggcattat tttaatggga ataggaacag gcatggtgtt
     1861 ctatgcctat gatatgcccc atctggcggc gttgggaatt atcgcagggc tctaccacac
     1921 gcttaatcac gcggtgttta aagccttact ctttatgggc gcgggttcac tcctgtatgc
     1981 cacccacacc cggaacatgg aagaatatgg aggactcctt cgacggatgc cctggaccgg
     2041 cttatttttc ttaattgggg ccgtttccat ttccgccctt ccaccaacga atggatttgt
     2101 cagtgaatgg ctcgtgtttc aggggctctt tttaagttta gaacttccct ccctgttttt
     2161 gaaattgatg cttcccatcg ctgcggctct gcttgcgctt accggcgcgt tggctctggc
     2221 ctgttttgtc aaggcgtttg gtatttcatt cctggctctt ccgcgaagct cccaggcacg
     2281 aaaagctcta gaggttcctg tccctatgcg cgtcgggatg ggattcttag ccggcctttg
     2341 tctggtttta ggcttgggcc ccgcagtcgt ggtgcccctc cttgatcggg tgagtgaacc
     2401 ttttgtgggg atatccattg ccgggagagt gtttgaaatg ggtggatgga cgctcgcacc
     2461 ggtaagcaga gagttttcta gtctctccac tccagtcctg gcgattgtct tggccggtct
     2521 caccgggttg ggatggggac ttgcctatgt tctggggggg aaagggaaaa cgcgattcta
     2581 taaaacatgg gggtgtgggt tgaatgtgac cgcacgaatg gaatacaccg cgaccgggtt
     2641 cgtccagccc attaagcggg tgtttagcac catttatcaa ccgacggtca agcttgaaac
     2701 ggaatttctt gaagaatcac attatttcgc caagcggaga cattttgaat ttttcattga
     2761 acccattttt ctcaagtatt tgtatcatcc gatcctggga tttttttcaa ccctggccga
     2821 tcgttctcgt gtgatccaag gcggaaattt gtccttgtac ctggcctaca tttttgtgac
     2881 gttggtgttt ttattgatgg ttatggggta gagcatactc atgagcgcaa tcgatttcct
     2941 gctgattatt ctgcaaatat ttcttgtcct agggctttct cccttgattg tgggagtcat
     3001 ccgaagagtg aaagcccatc tccaatgtcg gcatggggca aacgtgttcc agccatatgc
     3061 ggatcttgcc aaactctttc gcaaagagcc ggttatttct acgacggcct cgtggatctt
     3121 cccagccacg ccgtacatcc tgttttcctc aacgttgtct gccagtctgc ttgtccccac
     3181 cattattgcg cccatgccgc tcaattttgc gggaaatatt attgccctgg tctatcttct
     3241 ggccttaggg acctttttcc tgatgctggc gggcctggat gccggatccg cctttggtgg
     3301 aatgggcagc agtcgcgaag cgattgtcgc ttcgctgacc gaaccagcct tgattctgtc
     3361 cttgtttgcc gtggctctta cggcgggctc ggccaatatc agtgccatcg tgtataagtc
     3421 cgcgatcttg cagggtattg tgctggatcc tccgcctcac ctaatggctt tggtggcact
     3481 gtttatcatt acgctggccg aaaccggtcg tgtgcctgtg gataatccgg cgacgcattt
     3541 ggaattgacc atgatccatg aagcgatgat tttggaatac tccgggcgat atctggccct
     3601 ggttgagtgg gcagccggaa tgaaactcat gatttttttg accttgctgg ccaatatttt
     3661 tgcaccttgg ggcattgcca cgaccttgtc cccgtcagct ctgggattgg ggttgttggt
     3721 ctttgtgggg aaagttgtgg ggttggccgt cgtggttggt gtgtttgaat gtatgtttgc
     3781 caaattgcgg ttgtttcgcg tgacggaatt acttggggca gcctttattc tgtcattatt
     3841 agcgctggtg tttttttata tcctgcgggt gtgacatgcc aactctcccg atcaatctcg
     3901 gttcatatct ggtcaatctg tgctcggccc tcctgttgct gacatgtttt gccatcgtcg
     3961 cacaacgacg cctgatggcc tgcgtggact tgttcgcgtt gcagtccgtg tttttggccg
     4021 tgacggcgac gttggtggcg tttttgaccg gcattcacca tattt
//
LOCUS       DATIAL010000019         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_971346, whole genome shotgun sequence.
ACCESSION   DATIAL010000019 DATIAL010000000
VERSION     DATIAL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971346"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VLA60_00610"
     CDS             complement(<1..329)
                     /locus_tag="VLA60_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07880.1"
                     /translation="MDLERLPTKPSGSQLVSRQGLSPLRVTGRPPQYESWAAVKIPTK
                     LYTATRLAIGFFLLFTLILVFVPWTQTITAQGQLSAYSPAERPQEVHAPIEGRIMTWK
                     INEGIMV"
     gene            373..513
                     /locus_tag="VLA60_00615"
     CDS             373..513
                     /locus_tag="VLA60_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07881.1"
                     /translation="MRRISPIGTEEMGSYAGVWFIDIPIRYLILGFSDRKAKSRHTLQ
                     YP"
     gene            577..1506
                     /gene="nhaR"
                     /locus_tag="VLA60_00620"
     CDS             577..1506
                     /gene="nhaR"
                     /locus_tag="VLA60_00620"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HSF07882.1"
                     /translation="MYASTMEWLNYHHLLYFWVIAREGSIKGACEELNLSQPALSAQL
                     RALEESLGEKLFDRVGRTLVLTEMGQVAYRYAGEIFSLGQEFTNLLKGRPTHRPLKLV
                     VGIAEVVPKMVAYKLLESVYALREPVQIVCWEGRLDRLLGELALHALDLVLADTPIPS
                     TVKIQGHSHLLGESGVALFATKKITAKYRKGFPKSLTDAPFLLPTSNSILRRGMDEWF
                     ARIGVVPRVVGEFEDGATMKAFGGAGQGIFPGSVVMAKGIARQYAVQKIGIVSGIREQ
                     FYAISAERRLKHPGVLAIVDSAEKTIFRRSGSA"
     gene            complement(1650..2057)
                     /locus_tag="VLA60_00625"
     CDS             complement(1650..2057)
                     /locus_tag="VLA60_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07883.1"
                     /translation="MKVFAYVLMGVLGGAGLGIFFTGSPSQHFLSSPMSYAMPTPETE
                     DVKPWTGNDLLEMAQLYDQQADELQSEAVRIEQRAASLMLKPHMDPKGFHRTMLMHVA
                     SARWKSARELRDLAVMHRQEGQRLLAFKSPEGP"
     gene            complement(2237..>2478)
                     /locus_tag="VLA60_00630"
     CDS             complement(2237..>2478)
                     /locus_tag="VLA60_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07884.1"
                     /translation="RALGRSHATLEQLDFPLASNRYPRVRGSAMTSAIYPEEGRALET
                     VRMIALHEALDQAVMKLAQEWTEGVGAPIPSSSTS"
BASE COUNT          593 a          593 c          640 g          652 t
ORIGIN      
        1 ttcaccataa tgccttcgtt gattttccag gtcataatgc gcccttctat gggcgcatga
       61 acttcctgcg gtcgttcggc aggggaatag gccgatagtt ggccctgagc ggttatcgtt
      121 tgggtccagg gaacgaagac aagaatgaga gtgaacagaa gaaaaaaacc gatggcgaga
      181 cgtgtcgcgg tgtacaattt cgtcggtatt ttgaccgctg cccatgattc atactgtggc
      241 ggacgaccgg taacgcgcag cggagaaaga ccttggcggg aaacgagttg agagccagat
      301 ggttttgtgg gtaaacgctc aaggtccatc ggttactgtt tgtccgccaa atttcctggg
      361 gattcacccc acatgcggcg aatctcgcct atagggaccg aagaaatggg atcatatgcc
      421 ggagtgtggt tcatcgatat cccaatccga tatctcatat taggattttc ggatcgcaaa
      481 gccaagtccc gtcatactct tcaatatccc taattatcac cattggtgtc gacataaacc
      541 aattcataat ttcttcttga atcatttaaa aactgaatgt atgcttccac catggaatgg
      601 ttaaattacc atcatctgct gtatttctgg gtgatcgcgc gggaaggcag cattaagggt
      661 gcttgtgaag agctgaatct ctcccaaccg gcgttgagtg ctcagcttcg tgcgttggaa
      721 gagtccttgg gagaaaagtt atttgatcgg gtcggacgaa ccctggtctt gaccgaaatg
      781 ggacaggtgg cctaccgata tgccggggaa atcttttccc tggggcagga attcaccaac
      841 cttttgaaag gtcgaccgac tcatcgtccc ttgaaattag tggtgggcat tgccgaggtt
      901 gttcccaaaa tggtggccta caaactgctg gaatcggtat atgccctccg cgagcctgtg
      961 caaattgtct gttgggaagg acggttggac aggttattag gggaattagc cctgcatgcg
     1021 ttggatctcg tgttggccga tacccccatt ccctccactg tcaaaatcca agggcacagt
     1081 caccttctgg gagaatcggg ggtggcgtta tttgccacga aaaaaattac ggccaaatat
     1141 cgaaaaggtt ttccgaaatc gttaaccgat gccccatttc tcctaccgac gagtaattcg
     1201 atacttcgtc gcgggatgga tgagtggttc gcacgcattg gagtcgttcc cagagtggtc
     1261 ggggaatttg aagatggtgc taccatgaag gcatttggag gggccggcca aggcattttt
     1321 ccgggatcgg ttgtcatggc caaagggatt gcccggcaat atgcggtgca aaaaatcgga
     1381 atcgtttccg gtatccgaga gcaattctat gccatctcag ctgagcgacg gcttaagcac
     1441 cccggggtgc tggccattgt cgactcagct gaaaaaacca tttttcgacg ctccggttca
     1501 gcctgaaatg attttttgaa tttctcattt cgtgaaaata tctaaaatga catactgtct
     1561 ggcttcgccc tttccggtgg agcagatcca ggccgatagt ctttgactct caccaccctc
     1621 tggccatttg gcatggtttt ttctttgctt taaggccctt caggactttt gaaggctaag
     1681 agacgttgac cttcctgccg gtgcatgacg gctaaatccc ggagttcccg agccgatttc
     1741 caacgtgcag aggccacatg catgagcatg gtccgatgaa accctttagg atccatatgc
     1801 ggcttaagca taagggaggc cgcccgctgt tcaatgcgaa cggcttcact ttgtaactca
     1861 tcggcttgtt gatcatagag ttgtgccatc tcaagtagat cgtttccggt ccagggtttc
     1921 acatcttctg tttcaggtgt tggcatggca taactcatgg gagacgataa aaagtgttgt
     1981 gatggtgatc ctgtaaaaaa gatccccagc cccgctcctc ccaagacgcc catcaacaca
     2041 taggcaaaga ctttcatttt gctccctcct tgttgggtta gatttctgta aacacaacaa
     2101 atatggaaat attaacaaga aaatttataa gagtcccttt tcggaaggct atttggagcc
     2161 gtagggaatc actatgaccc cacgtcgggt tgtctccatc cttcattaca tccagaagtg
     2221 aggagtctgc acctgtttaa gaagtactgc tggaaggaat aggggccccg actccctcag
     2281 tccactcttg agccaatttc atgactgcct gatcaagcgc ctcgtgtagt gcgatcatcc
     2341 tgacagtctc gagagccctt ccttcttcag gataaattgc gctggtcatg gcagatcctc
     2401 tcactctggg ataccggttg gatgccagag ggaaatccaa ttgttccagg gttgcatgag
     2461 agcggcctag tgcccgga
//
LOCUS       DATIAL010000020         5091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_756588, whole genome shotgun sequence.
ACCESSION   DATIAL010000020 DATIAL010000000
VERSION     DATIAL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756588"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VLA60_00635"
     CDS             <1..719
                     /locus_tag="VLA60_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF07885.1"
                     /translation="RKAIAHAIDREHIIQFVLRGRARVAQTLFPPEHWAGHASLSGYA
                     YDPEEAKRLLKEAGYDHDHRPTITYKTSTDPFRLRLATIIQYQLDQVGIDVSIQSHDW
                     GTFYGDIKSGRFQMYSLAWVGVKSPDIFRYAFHSDAIPPVGANRGHFADVVADRLMIQ
                     AEQTLNRQEQQALYRTLQARLLDTLPYVPLWFEDHYALASTTITGYQLSADGNFDGLS
                     QVRWMGPPSVPPTHLARLYP"
     gene            790..1722
                     /locus_tag="VLA60_00640"
     CDS             790..1722
                     /locus_tag="VLA60_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF07886.1"
                     /translation="MTHFLVTRILSAMLALLGVICLVFLLIHLIPGDPVEVMLGESAQ
                     PTDKETLRHALGLDLPLHQQWWIYFKGLLHLDLGTSLFSGRAIVDLLVERIPATLYLS
                     LVSLLVAIGLALPLGLVAAVRQHTPLDYGAMGFALFGMSIPNFWMGPLLILIGALWLG
                     WFPVSGREGWNAVVLPALTLGTAMAAILARMIRSSVLEVLGEDFMRTARAKGLSSTRA
                     VLYHALPNALLPILTLLGLQLGGLLGGAVITETVFAWPGLGLLMIEAIQQRDYPVVQA
                     AVLCISMTYIVVNLLTDLLYAWLDPRIQFTTGEG"
     gene            2049..2858
                     /locus_tag="VLA60_00645"
     CDS             2049..2858
                     /locus_tag="VLA60_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF07887.1"
                     /translation="MRLWLPLSIIAAWAFLAIFNFMFSLTPDAIDLTNILSPPSLEHW
                     LGHDELGRPVADRLISGAHTSFFVSLCVVALGVSLGSLLGVSSAYLSGWVDAGFVRIV
                     DIMLAFPGLLLAIALAGALGPGIGNVILALGIVGWVGYARLARGQVLALKHADHVLAA
                     QAIGAPPWRIILRHLLPLIMAPLIVEASFGVAGAIIAEAGLSFLGLGVQPPAASWGSM
                     IRDGTRYMLVAPHLVVFPGLALMAVVLAANMLGDWLRDRLDVKEQTSRGGS"
     gene            complement(2966..3589)
                     /locus_tag="VLA60_00650"
     CDS             complement(2966..3589)
                     /locus_tag="VLA60_00650"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF07888.1"
                     /translation="MGLRILFLGIVFGAMVCTGGWESAQAAGEDESEVRMAPGTEVNF
                     SPEVVAGYIHAVIAADRALYTTHVVDRMQENRIVIAAEAWKQRKALPLPAQMLLMGGR
                     VVEMGGSGLRYRLASLWPIYEENGPSTVFEEAGLKVVAENPDEVYSGIIKRGDQRFFK
                     AIYADRAVSKACVDCHNGHLLSSKRDFKMGDVMGAIIISFPLPLEKK"
     gene            complement(3748..4668)
                     /locus_tag="VLA60_00655"
     CDS             complement(3748..4668)
                     /locus_tag="VLA60_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSF07889.1"
                     /translation="MKGRKIWKGIVGVGLIALFAQTGWSYDEITVTDGGTIQGKVTIT
                     GEKPRPMAFNLVTIPDPVFCGTISTGTGWRIVEDFIIGPDQSLKDVVVFLKDIEKGKP
                     FHMPKVRIESVDCEFIPFVNVLRDGDELTVVNMDPVEHDIQAYETARERGARVLFNRP
                     LPMNPFHKVAGIFGKKHLPGEPMVEKIHLRKGRNVFVMQCGFHPYMFSWGLVLENPYY
                     TITPEDGTFEITDVPPGDYMLTAWHPGMKEFVEQPITVTAQKATVANFQYEAPQGRRS
                     AHEIEENPRFGLELLEEGLEIVPSIRRQIP"
     gene            complement(4801..>5091)
                     /locus_tag="VLA60_00660"
     CDS             complement(4801..>5091)
                     /locus_tag="VLA60_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07890.1"
                     /translation="EPFLPGADMISTVEFSGSEGLDVFLPSAGGKWSLAGDYVWSNGR
                     LPYSQSGQWGYLRVLPSTDQRIQPLDGSAMSSKQAALDEPSGEPRIIPTVAK"
BASE COUNT         1084 a         1497 c         1248 g         1262 t
ORIGIN      
        1 tgcggaaggc tattgcccat gccatcgatc gtgagcacat tattcaattc gtgttgagag
       61 gacgagccag ggtcgcgcaa acgctctttc ctccggagca ttgggctggg catgcctcct
      121 tatcagggta cgcctacgat ccggaggaag caaaacgctt attgaaggaa gccggatatg
      181 atcatgacca tcggccgacc atcacctata aaacctcaac tgaccccttt cgattgcgct
      241 tggccacgat cattcaatac caactcgacc aggttggaat tgatgtctcc atccagagcc
      301 acgattgggg cacattttac ggtgacatca aatccggccg attccaaatg tatagcctgg
      361 cctgggtcgg tgtgaaatct ccggatattt tccggtatgc ctttcatagt gacgccatcc
      421 ctcccgtcgg cgccaaccgt ggtcactttg cagatgtggt ggcggatcgt ctcatgatac
      481 aagctgaaca gaccctgaac aggcaggagc agcaggcatt gtatcgcacg cttcaagccc
      541 gtctcctgga tacccttccg tatgtgcctc tctggtttga agaccattat gctctggcgt
      601 ccaccaccat caccggatat caattgagtg ccgatggcaa ttttgacgga ctcagtcagg
      661 tccggtggat gggccctccc tcagttcccc ccacacacct cgcacggctt tacccatgac
      721 catgccgcgc gaccatgacc ggggcacgca ggctcttttt tttcaactac cgttttctcc
      781 ggtttcgtca tgacccattt cctggtgaca agaatcttaa gcgcgatgct cgccctcctg
      841 ggagtaattt gcctggtgtt tctgttgatt catcttatcc caggcgaccc cgtagaagtg
      901 atgctgggtg aatcagctca accgacagac aaagaaaccc ttcgccatgc cttaggcttg
      961 gaccttcccc tccatcaaca gtggtggatt tatttcaagg ggcttttaca tctggatttg
     1021 ggcacatcgt tgttttcggg tcgggcgatt gtagatctgc tcgttgaacg cattcccgcc
     1081 accctctatc tcagtctcgt ctccctgctt gtggcaatcg gcttggccct tccccttggc
     1141 ctcgtcgcgg ccgtccgcca acatactcct ctggattatg gcgccatggg gtttgcgttg
     1201 ttcggaatgt cgattccaaa tttctggatg ggtcctttac tcattttaat cggcgcgctc
     1261 tggcttggct ggtttcccgt gagtgggcgg gaaggatgga acgccgtcgt gctgcctgcc
     1321 ctcacactcg gaacagccat ggcggccatc ctggccagaa tgattcggag cagtgtcctt
     1381 gaggtattgg gggaggactt catgcggacc gctcgcgcca aaggcctctc ctcaacccgg
     1441 gcggtcttgt accatgcact ccccaatgcc ctcttaccca tcttgacact cctgggcctt
     1501 cagctcggag gcttgcttgg tggagcggtg atcactgaaa cggtttttgc ctggccggga
     1561 ctaggcctgc tgatgattga agccattcaa cagcgcgact atccggttgt gcaagcggca
     1621 gtgctctgta ttagcatgac ctatatcgtg gtgaatttgc tcacggatct cctatatgca
     1681 tggctggacc ctcgcatcca attcaccact ggggagggtt gaaaaagtcc gccagcggcg
     1741 ttccctgcct ttgccgggcc tccttcgccg aagttgctac gaaggctgga agcggcttcg
     1801 cgtctacgcg ctgaagcacg cttcggcgca caagcgtgca ggcaggtcac ccctttgtca
     1861 tggataggta ctcacctgta cgttccgcgt ggcaaacggg ctgcggcctt cccttcggca
     1921 tggctcaggg caggacggga cggacctctt tgaaccctcc cttattgctc ttgatagcca
     1981 cgcaataata tggagaagat tgaaatgggt ggaaatctga attattcaac agtcaccact
     2041 ggctaaccat gcgtctttgg ctcccactct ccatcatcgc cgcgtgggcc tttctggcca
     2101 tcttcaattt catgttttcc ctaacccctg acgcgattga tctcaccaat attctttccc
     2161 cgccaagtct cgagcactgg ttggggcatg atgaattagg tcggccggtt gccgaccgtc
     2221 tcatcagcgg cgcacacaca tcgttttttg tttcgctgtg cgtggtcgcg ctgggggtga
     2281 gtcttggaag ccttctcggc gtgtccagtg cgtatctcag cggatgggtc gatgcaggct
     2341 ttgtgcgaat tgtggacatc atgctcgcct tcccgggttt actgcttgcc atcgccctgg
     2401 ccggggcatt gggaccggga atcggcaatg tgattctggc gttgggaatc gtgggatggg
     2461 tgggatatgc ccgactggcg agaggacagg tcctcgccct caagcacgcc gatcacgtgc
     2521 tggcagccca agccatcggt gcaccgcctt ggcgtattat tctccggcac cttctgccgt
     2581 taatcatggc cccgcttatt gttgaagcga gttttggtgt cgccggcgcc atcatagccg
     2641 aagccggcct ctcctttttg gggctggggg ttcaacctcc tgccgcatca tggggaagca
     2701 tgatccgcga cgggacccgc tacatgttgg tggctcccca cctggtcgtt tttcccggct
     2761 tggctcttat ggcagtcgtt ctcgcggcaa acatgctggg agattggctg agagatcggt
     2821 tggatgtgaa agaacagact tcccgaggag ggtcatgaaa catgtcgaac ggcagacagg
     2881 aggtgactgc gggtccagca cccacagcca ccttcctgac ttcgaattca aaaatgaacg
     2941 aaaggtattt gatcgttcag ggtgtttact ttttttctaa aggcagcgga aacgagatga
     3001 tgattgcgcc catcacatct cccattttaa aatcacgctt ggaactcaac aaatgcccgt
     3061 tatggcagtc cacacaggct ttcgaaacag cccgatccgc ataaatggcc ttgaaaaacc
     3121 gttgatcccc tctcttgata attccactat agacctcatc gggattctca gccacgactt
     3181 ttaatccggc ctcttcaaag acggtgctcg gcccattttc ctcataaatt ggccacagac
     3241 tggccagacg atagcgcagg ccggacccac ccatttcgac cactctacca cccatcagaa
     3301 gcatttgcgc cgggagtgga agcgccttcc gttgcttcca ggcttctgcg gcaatcacga
     3361 ttcgattctc ctgcatgcga tccaccacat gcgtcgtata gagcgctctg tctgcggcaa
     3421 tcaccgcatg gatatacccg gccacaacct ctggagagaa attcacctca gtccctggtg
     3481 ccatccgcac ttccgattca tcctcacccg cagcttgcgc gctttcccaa ccgcccgtgc
     3541 acaccattgc cccaaacacg attcctaaaa acaatatccg taaacccatg gtcgccctcc
     3601 tttcatccgt catgacttca gtgaacgaat caacatcctg ttgccttgct tcaagcgggc
     3661 tacctcagga gatgcctcct gttccttgac gatcggtcat caaccctcgc ctgccggggc
     3721 accactcgtg gcacgcatga caacccgtta aggaatctgg cgtctaatcg aaggtacaat
     3781 ttccagacct tcttccaata actccaatcc aaaccgagga ttctcctcta tttcatgcgc
     3841 actacgacgg ccttgtggag cctcgtactg aaaattagcc acagtggcct tctgagccgt
     3901 caccgtgatg ggttgttcca caaactcctt catcccggga tgccaggcag tcagcatata
     3961 gtctccagga ggaacgtcgg taatttcaaa ggttccatcc tcgggagtaa tcgtataata
     4021 ggggttctcc aacacgagcc cccaggaaaa catataggga tggaatccgc actgcatgac
     4081 aaacacattc cgtccttttc gtaaatgtat tttttccacc attggttccc caggaaggtg
     4141 tttcttaccg aatattcccg ccaccttgtg aaacggattc atcggcaggg gacgattgaa
     4201 caagactctg gcccctcgct ctctggccgt ttcataggcc tgaatatcat gctcaaccgg
     4261 atccatgttg actacggtca gttcgtcccc atcgcgcaat acattcacaa acggaataaa
     4321 ttcacagtcg actgattcga tcctgacctt gggcatgtgg aatggtttcc ctttttcgat
     4381 atctttgagg aagaccacca catccttaag gctttgatca ggcccgataa taaaatcctc
     4441 aacaatacgc caacccgttc ccgtagagat cgttccacaa aacaccggat cgggaatcgt
     4501 cacaagatta aaggccatgg gtctgggctt ttctcccgta atcgttacct tcccttgaat
     4561 ggttccccca tcagtcacgg ttatttcatc gtaggaccaa cccgtttgcg cgaaaagcgc
     4621 tataagccca acccctacga tccctttcca tatttttcgg cccttcattc tttcctccca
     4681 caaaaaataa aatgattgtc atacttatga tcattctatc gaaccaccca ctcaaaaaaa
     4741 agcggggaag ccgaagagtg gcttccccgc tttcgctaac cctcggaatg agggtctggc
     4801 ttacttcgcg acggtaggaa taattcgcgg ctcgccactg ggttcatcaa gagccgcctg
     4861 ttttgaagac atggccgaac catccaatgg ctgaatccgt tgatccgtgc tgggcaacac
     4921 ccggagatag ccccattgtc ccgattggga atagggtaac cggccattac tccacacata
     4981 atctccggcc agggaccatt tgcctccagc cgagggcagg aagacatcca acccttccga
     5041 acccgagaac tctacggtgc taatcatatc cgcaccgggc aggaaaggtt c
//
LOCUS       DATIAL010000021         8519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1401955, whole genome shotgun sequence.
ACCESSION   DATIAL010000021 DATIAL010000000
VERSION     DATIAL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401955"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VLA60_00665"
     CDS             complement(<1..1204)
                     /locus_tag="VLA60_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSF07891.1"
                     /translation="MPRFEYRAKNLDGQTVHGEVLAATSSEALQLLRRQDVLVIGLQE
                     KVERVFNLSGQLTGWSRRWSWRGVSSKELVVFTHQLATLIRAGVPLLECLDILSSEAE
                     NPTLQQVVKHIREDVEGGTLLAHALKRNPTVFNEFYRSMVEVGETTGRLDESLTQLAV
                     YLDKQAQLRAKIFSGLAYPALLVAVAMIVLVFLLIWVVPLFSGLFQDMGESLPWLTQV
                     VIDVAEGVRDHFFLLAAFWGSLVMGIRWILKNPKSRQAIDGWVLGVPLLGAVIQKAAT
                     VRFSRTLGFLVRRGVPLLSALSVAGTVTGNKIFEQSINLAAIAIQNGRPLSETLRAGH
                     VFPPMVPQMIKVGESTGSIDVMLEKIADLFEQEVDRTVATLTSVLEPCIILVVGCGIA
                     LVVVAMYLP"
     gene            complement(1259..2371)
                     /locus_tag="VLA60_00670"
     CDS             complement(1259..2371)
                     /locus_tag="VLA60_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HSF07892.1"
                     /translation="MELTALLGELCRQGGSDLHLVSGSVPRLRVDGHLRPMESPTLTS
                     QDMSLLTASLLTEAQHQQLMKTGAWDGAYSVPAIGRFRVHVYTQQGCLAMAIRTVSGK
                     IPTFEELGLPPIISELMRKPQGLILVTGPTGSGKSTTLASMLDHINEERSAHIISLED
                     PIEILHSHKKSLVSQMEVGSDVREFQSALKGILRQDPDVVFLGELRDLKTIQAALTMA
                     ETGHLTVDTLHTNSAIHTLTRLVSVFPSHQQQEIRIQLSMVLEGILAQRLLPRSEGRG
                     RVLALEILITSPAIRNLIREDKIHQMYSMMQTGQAQYGMQTMNQALADLAGQGVISSE
                     LAMGLTTLPDELSKLLERTGRERSGALSLARLRPRM"
     gene            complement(2396..4213)
                     /locus_tag="VLA60_00675"
     CDS             complement(2396..4213)
                     /locus_tag="VLA60_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSF07893.1"
                     /translation="MNRERVGLQLVDAKVISSDDLSQALDIQQGEGGRLGSILVRMGV
                     LSESTLLEFLSQHYGVATVELSTCSIDGSLRGLVPYDVVRRHLVLPVRKTTSRLSLAM
                     ADPTNASLLDDLRFRTGLHIIPMVATESDLRTAISHVYGQGPDGSSSTTEVVQKDEEG
                     TFKNCGNGDRISPNSMGKPVGPVCASANGLPDQERSIMLQDKAEMSRVKRHAKIDKDS
                     SAVEVVNGLVQQAIEMEASDIHIEPMETMIRVRFRLDGVLCPIQNLPKDLHQALLARV
                     KILSDLDIAERRLPQDGRMKIEGFPHVDIRVAILPCLFGEKAVLRLLNQSGLALHLTN
                     IGLSQPDLDRLTTALENPYGMILVTGPTGSGKTTTLYSALQFLNTPQMNIITVEDPVE
                     YQIQGINQMQIHEEIGLNFAAGLRAFLRQDPDVMMVGEIRDRETAQIAIQASLTGHRV
                     LSTLHTMNAPGAITRLIDMSIEPFLVSSAVSLIVGQRLVRKICDHCRELEPVSQFQLR
                     ELGFDEHALGAVQAMKGRGCVSCHQTGFKGRMALFETLPIFEGLHEKILARASTNDLI
                     TCAIGEGFRTLRQAGMVAVQGGLTTVGEVFAETRSDAFV"
     gene            complement(4234..4806)
                     /locus_tag="VLA60_00680"
     CDS             complement(4234..4806)
                     /locus_tag="VLA60_00680"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSF07894.1"
                     /translation="MVRESSGADIPINLPNSLTVLRILLVPVFVGFLLYEYYDYALVT
                     LLVAAVTDGLDGAIARITDQRTRLGEYLDPLADKLLLMSAIVTLSVLHFIPIWAVILV
                     VSRDAILLTGTILANLTEIDIDITPTWLGKGTTFAQICYVIIVILFATGRVPSESIIP
                     FLSVMVILTTGSGVHYLFRGIQRLNSSGKK"
     gene            4875..5294
                     /locus_tag="VLA60_00685"
     CDS             4875..5294
                     /locus_tag="VLA60_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07895.1"
                     /translation="MKSTTLATPIEAAYNPLSLKEKIPLFIVKPEPEHMTSLPLSFRV
                     RNAVVEKHQLEGMDPSDRYFNRMIPIKRVERGYSGTVMYEALNLQSQVYRTVQETLKD
                     LIDQLRELGFTTMRTRLNFKGQQYLAEKETWVDYLDA"
     gene            6257..7138
                     /locus_tag="VLA60_00690"
     CDS             6257..7138
                     /locus_tag="VLA60_00690"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSF07896.1"
                     /translation="MDLSTVLNGLDHFFTYPLFTVNKTPITLSSLAFFAFIMGGFMII
                     NTLIRRFLSNQLLKRSKIPKATQYTLTRIIQYFLILIGTVIAFQVIGVDLSGLVVIFG
                     FLSVGIGFGLQNLTSNFIAGLMLLFEQHIQIGDRITVGDTEGDVAEINIRSTTIRSLN
                     NVAIVVPNSEFISSTVINWSHGDPKTRLEIEVGVSYNSDLDKVISSLLEAAKENPMVL
                     PHPEPKAWLMSFGDSAWNMRLLAWVEDPQGRRQVQSDINCAIVKKFRKNGVEIPFPQR
                     DLHVRTSVPLSIASLST"
     gene            7592..8068
                     /locus_tag="VLA60_00695"
     CDS             7592..8068
                     /locus_tag="VLA60_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07897.1"
                     /translation="MKSLMVYMAVFVLGVGLGLGAPFLAARYAQPYIPQFLQETVHPL
                     TGTVTHKERQQDRLLMTLTTRDGTILATFRKQVQEIDLLVEEQDSVTLDVKQYEPFVT
                     DPPVIKVNKLTRQVPEPSQPSQSSIESEPLPDSPLNQVPASGEPSEDTEPSHLPLN"
     gene            8293..8418
                     /locus_tag="VLA60_00700"
     CDS             8293..8418
                     /locus_tag="VLA60_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07898.1"
                     /translation="MDITLWTATLEESFEYSFKLFMAYLPKGLGALMLLGMGILL"
BASE COUNT         2199 a         2413 c         1886 g         2021 t
ORIGIN      
        1 tgggaagata catcgccaca acgacaaggg caattccaca acccacgacc aggataatac
       61 agggctctaa caccgacgtt aacgtagcta ccgtccgatc gacctcttgc tcaaaaagat
      121 ccgcaatttt ttccagcata acatcaatgg aaccggttga ttctcccact ttaatcattt
      181 ggggaaccat cggaggaaaa acgtggccag ccctcaaggt ctcggagagg ggtcgcccat
      241 tttgaatcgc tatggccgcc aggttgatgc tttgctcaaa gattttattt ccagtcaccg
      301 ttcccgccac actcaaggcc gacagcaacg gaaccccacg acgaactaaa aaccccaatg
      361 ttctggaaaa acgcaccgtt gccgcttttt gaatcacggc ccccaaaagg gggaccccta
      421 acacccaccc atcaatggcc tgtcgactct tcggattttt gaggatccac cggatcccca
      481 taactaaact tccccaaaat gccgccaaca gaaaaaagtg atcccgaacc ccctcagcca
      541 cgtcaatcac cacttgcgtc aaccagggaa gcgattcgcc catatcttgg aataagccag
      601 aaaaaagagg gaccacccaa atcagtaaaa agaccaggac aatcatcgcc acagccacga
      661 gcaaagccgg atacgccaat ccggagaaaa ttttcgcccg caactgagcc tgtttatcaa
      721 ggtaaactgc caattgggtc aaactctcgt ccaagcggcc cgtcgtctct cctacttcca
      781 ccatactccg gtaaaattca ttaaagacag tcgggttgcg ctttaaagca tgagccaaca
      841 aggttccacc ctccacatcc tctcggatat gtttcaccac ttgttgcaag gtcgggtttt
      901 cagcttcgct ggagagaatg tccaggcatt ccaaaagagg cacacccgca cggatcaacg
      961 ttgccagctg atgggtaaag actaccaatt ctttactgct cacgcctctc cagctccacc
     1021 ggcggctcca gccggtcagc tggccactga gattgaatac cctttcaact ttttcctgca
     1081 gaccgattac caagacgtcc tgcctacgaa gaagctgcaa ggcctcactg gaagtagcag
     1141 ccaatacttc tccatggacc gtctgtccat ccaaattttt cgcacgatat tcaaatctcg
     1201 gcatttccaa ttattgctcg cttgccctgt ggaagatcaa gaaggctctc aatgacattt
     1261 acatccgagg acgcaacctg gccaaggaca acgccccgga tcgctcccga cccgtgcgct
     1321 ccaatagttt ggataactca tcaggaagcg ttgtgagtcc catagcgagt tcagaggata
     1381 tcaccccctg gccagccaag tcggccaacg cttgattcat ggtctgcatg ccatattgag
     1441 cttgccctgt ttgcatcatg gaatacattt gatggatctt gtcttcccga atcagattcc
     1501 gaattgccgg agaggtaatc agaatttcca aagccagaac ccggcccctt ccctctgaac
     1561 gtggcaaaag cctctgtgcc agaatgcctt ccagcaccat cgacaactgg atacgaattt
     1621 cttgttgctg gtgggaagga aacaccgaga ccaatcgtgt cagcgtgtga atcgcagaat
     1681 tcgtgtgcaa agtgtccacc gtcagatgtc cggtttcagc catggtgaga gccgcctgaa
     1741 tggtcttgag atctcgcaat tcccccaaaa acaccacatc gggatcctgc cgtaagatac
     1801 ctttcagtgc ggattgaaat tcgcggacat cggatcccac ttccatttga gataccaaac
     1861 tttttttgtg cgaatgcaga atctcaatgg gatcttctaa cgaaataata tgggcagacc
     1921 gctcttcatt tatatgatcc aacatcgagg ccaatgtcgt acttttcccg ctaccggtcg
     1981 gccccgttac caaaatcagc ccttggggct tcctcatcaa ttccgaaata atgggcggta
     2041 gccctaattc ttcaaacgtc gggatcttcc cggacactgt tcgaatggcc atggccaagc
     2101 atccctgctg ggtatagaca tggacccgaa accgaccgat agctggcacg ctgtaggctc
     2161 catcccaggc tccggtcttc ataagctgtt gatgctgtgc ttcggtcaac agactcgcgg
     2221 ttaacagaga catgtcctga ctggtcaatg tcggagactc catcgggcgt aaatgcccgt
     2281 ccacacggag gcggggcaca ctgccggaaa ccaggtgcaa atccgatccg ccctgccggc
     2341 acaattcacc taggagtgca gttaattcca ttgggcatcc gttcttctga cgtccctaca
     2401 caaaagcatc agatctggtt tcggcaaaca cctcaccaac agtcgtcaag ccaccttgaa
     2461 ctgccaccat ccccgcttgt ctaagcgtgc gaaatccttc gcctattgca caggttatca
     2521 agtcattcgt tgaggcccgt gccaaaattt tttcatgcag cccttcaaaa ataggcaggg
     2581 tctcaaataa agccatgcgt cctttaaatc cggtctgatg gcaggataca cacccacgcc
     2641 ctttcatcgc ctgaaccgcc ccgagcgcat gctcatcaaa acccagctcc cgcagctgaa
     2701 attgagatac cggctccagc tcccgacaat gatcacaaat tttccgcact aatcgttggc
     2761 ccacaatgag cgagacggcg gaagacacca aaaacggctc aatgctcata tcgataagcc
     2821 gggtaatcgc ccctggggca ttcatggtat gcagggtcga aagaactcga tggccagtca
     2881 gagaggcctg aatcgcgatt tgcgcggttt cccgatcacg aatttccccc accatcatga
     2941 cgtcaggatc ctgccgcaaa aaagcacgca acccggcggc aaaattcaat ccaatctctt
     3001 catgaatttg catctgattg atgccctgga tttggtattc cacgggatct tcgacagtga
     3061 taatattcat ctgaggagta ttcagaaatt gtaacgcgct atagagggtt gttgtcttcc
     3121 cgctccccgt tggccccgtc accagaatca tcccataggg attttccaga gccgtcgtca
     3181 accggtccaa atcaggctgg ctaagcccga tattggttaa atgtaacgca agccctgact
     3241 gattcaagag acgcaggacc gctttttcgc caaataaaca gggcagaatg gccactcgaa
     3301 tatcgacgtg gggaaagccc tctatcttca tccgcccatc ttggggtaac cgacgctccg
     3361 caatatccag atcggaaaga attttcaccc tggcgagtaa tgcctgatga aggtctttag
     3421 gcagattttg aatcgggcac aagaccccat ccaaccgaaa gcgaacccgg atcatggtct
     3481 ccatcggctc aatatgaata tcactcgcct ccatttcaat ggcctgctgc acaagcccat
     3541 tcacaacctc cacagctgaa gaatctttat caattttggc atgacgcttc acgcgagaca
     3601 tttctgcttt gtcctgaagc attatagatc tttcttgatc aggcaaaccg ttagcagagg
     3661 cacagacagg gccaacaggc tttcccatcg aattgggtga aatccggtcg ccatttccgc
     3721 agtttttgaa agtaccctcc tcatcttttt gcacaacttc cgtagttgag gaagatccat
     3781 ctggaccctg gccatataca tgggagatag ccgtccgtaa atccgattcg gtagctacca
     3841 tggggatgat gtgcaaccct gtccggaatc gcaaatcatc taacaaagac gcattcgtgg
     3901 ggtcggccat agccagactc agtcgtgaag tggttttccg caccggcaaa acgaggtgtc
     3961 ggcggacaac gtcataagga accaaccccc gaagacttcc gtcaatcgag cacgtagaca
     4021 attctacggt ggcaacccca tagtgctggc tgagaaactc taataaggtg gattcagaca
     4081 gaacccccat ccgaaccaga atgctcccga ggcgccctcc ttctccctgc tgaatatcaa
     4141 gcgcctgaga caaatcatct gaagagatca ctttcgcatc caccaactgt aggccgaccc
     4201 gctctcgatt caactgcatc ctcctgttta cccttatttc ttgcctgaag aattaagccg
     4261 ttgaattccc cggaatagat aatgcactcc agaaccggtt gtcaggataa ccattaccga
     4321 gagaaaagga atgatgcttt cggaaggaac acgccccgtc gcaaagagta tcactatgat
     4381 gacatagcaa atttgggcaa aagtggtccc ctttcccaac caggttggag taatatcaat
     4441 gtcgatttcc gttaaattag ccaatatggt ccccgtgagg agaatggcgt cacgactcac
     4501 cacaagaatc accgcccaga tcggaataaa gtggagaaca gataaggtca cgatggccga
     4561 cataaggagt agtttgtcag ccagaggatc gagatactcg ccgaggcgag ttcgctgatc
     4621 ggtgatacgg gcaatggccc catccaggcc atcggtcacc gccgcgacta ataatgtcac
     4681 gagggcataa tcatagtatt cgtagagcaa aaaccccacg aagacgggaa caaggagaat
     4741 tctgaggacc gtaagactgt tgggaaggtt gattggaatg tcggcacccg atgactcgcg
     4801 gaccatttcg ccacaacccc tttcttacca acgtattttt cctaagcgta gggaaggtta
     4861 cgttgggatt ctgaatgaag tcaaccacgc ttgcgacccc catcgaggcg gcctataatc
     4921 cactttcact aaaagaaaaa ataccgctat tcatcgttaa gccggagcca gagcatatga
     4981 catcgcttcc tttatccttt cgagtcagaa atgccgtcgt ggaaaagcat caattggagg
     5041 gcatggaccc aagtgaccga tattttaatc gaatgatccc aattaaacgg gtggaacgcg
     5101 gatactcggg aacggtcatg tacgaagcgc taaatctcca gtcccaggtc tatcgaaccg
     5161 tgcaggaaac cctgaaagac ctcatcgatc aacttcggga attaggcttt accaccatgc
     5221 ggacccgcct caatttcaaa ggccaacaat acctggcgga aaaagaaacc tgggttgatt
     5281 atctcgacgc ctaatcttgg ggctcccacc aaccattagc atgatgacct ttctattcca
     5341 aggacccttt cccgatctct gacaatagcc acatgttgtt cgttggggcg ttttcgccac
     5401 cgcacccgga gccgaacggc cggggcaaac cccataggag gcttcgtgga acgctggctc
     5461 ccccaggagc tccagatacc gcgtcaccac cgcctgagcc atccgatctc caatgatcgg
     5521 aatacccggc tgcctgaggc ctccctccgc tttcggatct cacccgaagc acggacggtg
     5581 ccacggaact gcccgacgac atcctgggcc agatctcata ccgctgatta atgagatccg
     5641 catcaacatc ccacattttc tgcccgtcta cacccgggcc accggcctta cgattcaggg
     5701 caagataggc cttcccacca gcgccttcgg taggtcagag gcttttgccg aattcatgcc
     5761 ttgctccccg cacgggttgc cccatgatcg cgcttaaatg acgtagcccc ttcgctcctg
     5821 atcgttcaac cccttcctca ctagaacgga cggctctgct gccttacgag ttgcacggct
     5881 tctcacctat aattgggggg aaaactttat gctgtctgtc ttatttctca cctcgtttgt
     5941 gttgaagtgt atccccctca cgaatgtctc gtgtgttttg accacttcaa actctgttcc
     6001 ttccgacttt tctttaaccg catcagacct taacgccaag ctataccgcc ttgtcccctc
     6061 gtaatctttt aatctcaagt ggcaagattc atctgtcata tgtttttctt tccgctttgt
     6121 tctcctctat cttttctgac gtttttctgg tttattcacc taacccgaca cggcaataaa
     6181 cgggaagcct ccgaaagagt ctcttcatca atcacaacgt gcccataaat tcataacgcc
     6241 tcaaaggagc accaccatgg atctttcaac ggtcctgaac ggattggatc actttttcac
     6301 ctatccctta tttaccgtca acaagacccc gattaccctt tcctccctgg cattttttgc
     6361 gttcatcatg ggtggcttta tgatcatcaa caccctgata cgacgttttc tcagcaatca
     6421 attactcaaa cgctctaaga tacctaaggc aacacaatac accttaaccc gcataattca
     6481 atacttcctg attcttatcg ggacggtgat tgcctttcaa gttatcggcg tcgacctgag
     6541 cggtctggtc gtcatttttg gatttctctc cgtcgggatc ggattcggcc tgcaaaatct
     6601 gacgtccaat ttcatcgcgg gactcatgtt gctgttcgaa caacacatcc agattgggga
     6661 ccgcatcacc gtgggagaca ctgagggaga tgtggcagaa atcaacattc gatccaccac
     6721 cattcgctct ctgaacaacg ttgccatcgt tgttcccaat tccgaattta tctcttccac
     6781 ggtgatcaac tggtcgcacg gtgaccctaa aacccgcttg gagattgagg tgggagtttc
     6841 ttacaattca gatttagaca aagtaatcag ttcacttttg gaagcggcaa aagaaaaccc
     6901 tatggtttta ccccacccgg aaccgaaggc ctggctgatg agtttcggag attccgcctg
     6961 gaacatgcgt ttattagcct gggtggaaga tccacaaggc cgaagacagg ttcaatccga
     7021 tattaattgc gccatcgtca agaaatttcg gaagaatggt gtggaaatcc ctttccctca
     7081 aagagatctt catgtccgaa cctccgtacc tttgtccatc gcatccttat cgacctgaac
     7141 atagggccca aaccgcaatc ggagcaggaa ggtttcttcc gacggctgta agaaatgcct
     7201 ggacgttact cctcttgcat gcagcttcca catgaacccc atgctatttg agaagctctc
     7261 atagcattgc tagaataata cgaagttgag atgtctccaa aaaattgcag acatcacttg
     7321 tgttcttcgt accccggttt gaaatgttgc agtcattctt tcatactgat gctctacgaa
     7381 cattcaactg atcacgatct aggcgccatc cacgatatcc caacaattga aacacttcaa
     7441 tttcaagtga acgaaagtac tacatgaccg gaaacaccca gtgtgactgg gggtcgttca
     7501 tttgcacgca ggaacattcg tcttcgtcag agtcttcgta taaaaaagga agaagcccaa
     7561 cagtcctact gtaaccgcgg agaaaacgtg aatgaaatcc ttaatggtct acatggcggt
     7621 gtttgtctta ggagtaggat tgggtttagg ggcaccgttt ctcgccgccc gatatgccca
     7681 accctatata ccccaattcc ttcaggaaac cgtccacccc ctgaccggga ccgtcacaca
     7741 taaggaacgc cagcaggacc gattgttaat gactctcacc acgcgagatg gcaccatcct
     7801 ggcgaccttt cggaaacagg ttcaggagat tgacctgctt gtcgaagaac aggactctgt
     7861 cacgttggac gtcaagcaat atgaaccttt tgtgactgac cctccggtca ttaaagtcaa
     7921 caaactcaca cggcaggtcc ccgaaccgtc ccaaccctca caatcttcga tcgaatccga
     7981 acctctgccg gactctccgt tgaaccaggt ccccgcatcg ggagagccat ctgaggacac
     8041 agaaccttct catctccctc tcaattaatg gctgagggcc caggggaaga gaaaacccca
     8101 cgggataata tgtgggctca gcaacatcac gtaccagtcc ggtaaggaca ttctctctca
     8161 ggatgatcga acgccacgtg aaacttatcg gtccagccgg cggcatgagg actatcgtct
     8221 ctctaatcgt ccagctcgtt atcggaatgt ttatcgtatt tctgcttatt tatttctaag
     8281 gaggtcaatc ccatggatat caccctttgg accgcaaccc ttgaggaatc gtttgagtac
     8341 agtttcaagc tgtttatggc ctatctcccc aaaggcctag gggccttgat gttgttaggg
     8401 atgggaattc tcctgtgaaa aatcgttgaa gcgggaacga gccgcgtatt gcatatgatc
     8461 ggcgtcgatc gtttactggg cggcaccggt gttctcacgc tgctcaaaaa aatcggtag
//
LOCUS       DATIAL010000022        13567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1294525, whole genome shotgun sequence.
ACCESSION   DATIAL010000022 DATIAL010000000
VERSION     DATIAL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13567
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294525"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1250)
                     /locus_tag="VLA60_00705"
     CDS             complement(603..1250)
                     /locus_tag="VLA60_00705"
                     /inference="COORDINATES: protein motif:HMM:NF022620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 family protein"
                     /protein_id="HSF07899.1"
                     /translation="MIKSGMNVPSWRAILASSAAGLVVTMFSVSCSTPASLPPKIAVG
                     LDGLRNEAVSLRGQIEKTVGALNELMSKPQADLSPQFQSYTHELGILEDRIEKARQQR
                     GATETAVKEQFMAWDENLKQLHNEATRAGAAERRADTDATYSDIKQKIVTLRNEANPF
                     LNDLKDVRQYLKSDLTKEGLSTIEPTANRVFDQKDTIINRLDEVIQSLDSAIKSR"
     gene            complement(1797..1946)
                     /locus_tag="VLA60_00710"
     CDS             complement(1797..1946)
                     /locus_tag="VLA60_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07900.1"
                     /translation="MGNSGSPAEAIKQLAEEVPSPGSDLVRARFTNGTFFGAGANTNL
                     RTFLI"
     gene            2297..2842
                     /locus_tag="VLA60_00715"
     CDS             2297..2842
                     /locus_tag="VLA60_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07901.1"
                     /translation="MKRLVSGIVTMTVLIAFTLMLGGYGHAEEGSVKAMSPWKGLGFA
                     FPVGPDQVYMVAVYSGVMLVDDGKGPMHAASIVCPGTVDADLKKGTKSGHAKCVITGE
                     QGNRVFAEFNCTGDVEGCRGTFTITGGTGRFAGITGEGEMISKIQAREITMVSGVESA
                     QQLGEGVAVWPNLTYHIPDKN"
     gene            2880..3350
                     /locus_tag="VLA60_00720"
     CDS             2880..3350
                     /locus_tag="VLA60_00720"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HSF07902.1"
                     /translation="MRTYLILGLKFGLGVLLLAPPLQAQTFVFPEKGQSPEQQELDDF
                     TCYKWAKQQTGYDPEHPNLAAAPPPPSGGGGMRGAMGGAALGAIGGAIAGNAGQGAAI
                     GAAAGGGLGMMRQRRAERGYQEEVQQASAKNQQTRATFDRAHNVCMEAKGYKIG"
     gene            3393..3917
                     /locus_tag="VLA60_00725"
     CDS             3393..3917
                     /locus_tag="VLA60_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07903.1"
                     /translation="MRVQQSCIILSCAVMLAGCVGAQQVGPVSSSYPVDSAPPPQAKT
                     FVFPEKGQGPEQQEQDDFTCYKWAKQQTGYDPEHPNLATAPPPPSGGGALFGAMGGAA
                     LGAIGGAIVGDPGMGAAIGAAAGGGLGLMGQRAAEQEYQQEVQQAGARNQRTRETFDR
                     AHRTCMEAKGYKVG"
     gene            complement(4175..4363)
                     /locus_tag="VLA60_00730"
     CDS             complement(4175..4363)
                     /locus_tag="VLA60_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07904.1"
                     /translation="MPLALCLNEFCRIKGRMELPLLLDHAGYLPPFGHDHGWQRARCN
                     SARQPVIIPDTIIINDME"
     gene            4432..4563
                     /locus_tag="VLA60_00735"
     CDS             4432..4563
                     /locus_tag="VLA60_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07905.1"
                     /translation="MSRDTLLEPTEGLSEVPHDRWLLAEGRDEPDGPLNPVDPIRSA"
     gene            4754..5314
                     /locus_tag="VLA60_00740"
     CDS             4754..5314
                     /locus_tag="VLA60_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07906.1"
                     /translation="MRKGIIVFGIWGLVGLQIGCTPLPGSDLVSSKSNGTKMVTTSTQ
                     FDEIADNSSSYLGQEKKLAGAVVSIDQTDEGYMILAEWLPYPKTQLEEGPKVEQSDRS
                     RHFLIRFIGKREKEFYTTRGNIFLLEGTVEGTKKALVNVFGPRKDLLYVNAKCVRIWE
                     TGQDTDRSSQLDNPYPPTRNRTLCAD"
     gene            complement(5567..5848)
                     /locus_tag="VLA60_00745"
     CDS             complement(5567..5848)
                     /locus_tag="VLA60_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07907.1"
                     /translation="MNGRLWWVVFALLSACGPTLQWVKPGATESDLQAAITLCNRENI
                     HFGRGNMRTLDPDGEVTVTSRQKRYERGAGEMMREKCLESHGWTREYVE"
     gene            complement(6060..6899)
                     /locus_tag="VLA60_00750"
     CDS             complement(6060..6899)
                     /locus_tag="VLA60_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07908.1"
                     /translation="MASQKINKSWVIAGILLGFMGSQTAHAEDIKKLAEEVQNPVSDL
                     VRVGFTNSTLFGTGFNNHLSNVFNLEAGNTRRFGQWALLNRLTIPILYFPANAIGDKS
                     GSLTGLGDIEFTPFLARDESKRLLKLIAGFGPTFILNTATDDRLGRGKWSVGPTLALV
                     SIPDPWVMGALIRNIWSFAGDNQRLKVNQFVIQPFLNYNFPNGWYLTSTPAIIANWEA
                     EDKRNRWTVPLGGGFGKVMFRGEKRPINIKLQGFYFLEKPDLTPDWTLQLQFQILFPD
                     KPA"
     gene            complement(7871..8917)
                     /locus_tag="VLA60_00755"
     CDS             complement(7871..8917)
                     /locus_tag="VLA60_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HSF07909.1"
                     /translation="MDTSGQLGFFDLTHRYDALTQKGDPLVQLAQRIPWVRFRKTLEK
                     GLQRSTRSKGGRPPFDALLMFKVLVLQALYNLSDDQTEYQIRDRLSFMRFLGLDLHQR
                     IPDAKTIWLFRETLAQAGVVEPLFAQFDTYLANQGLQARGGQLIDASLVPVPKQRNTR
                     EENATVQAGECPADWESQPAKRRQKDTDARWTKKHGVSHFGYKNHVNVDKQHKLIRQY
                     TVTDAAVHDSQVLEDVLQPKTAGRDVWADAAYRSDEIEGKLKTRKLRSQIQYRGYRDK
                     PLTPKQQQTNQRRSRIRARVEHVFGHHVTAMGGKLLRTIGRVRARAKIGLKNLTDNFQ
                     RFLVLTAAGREQTA"
     gene            9112..9702
                     /locus_tag="VLA60_00760"
     CDS             9112..9702
                     /locus_tag="VLA60_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSF07910.1"
                     /translation="MPAGDLTITPESQQLVDFSEPFLTNVREIIVTGPGSPTFSSLDD
                     LSGHTIHVRKSSSFYKHLVRLNASSKLAGQPEITLVPEEENLEDEDLLEMVNAGLLPM
                     LVMDAPLGEFWAKIFKNIRLHPDIAVNANGKIAWTFRKNSQKLAKVINQFARNNKKGS
                     LIGNMLFTRYLQNSPYVKNALATQERKKISTSHACV"
     gene            9743..9976
                     /locus_tag="VLA60_00765"
     CDS             9743..9976
                     /locus_tag="VLA60_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSF07911.1"
                     /translation="MALGYQESMLDQRKRSLAGAIGVMHLLPSSARDPNVSIPNINKL
                     EPNTHAGVKYLHFLHDRYFKDPHMDLLNQWLFR"
     gene            9961..10188
                     /locus_tag="VLA60_00770"
     CDS             9961..10188
                     /locus_tag="VLA60_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07912.1"
                     /translation="MAFSIASYNAGPAKIAKLRTAAAMGMNSNKWFKNVELVAAKRIG
                     RETVQYVSNIYKYFMAYRLILEKEAIKLAVY"
     gene            10245..10436
                     /locus_tag="VLA60_00775"
     CDS             10245..10436
                     /locus_tag="VLA60_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07913.1"
                     /translation="MTTFESRQRVTQHAEVITGMPPGYEGLGEYSRRCRFLSRGLLTT
                     VFPAPLAQAIADSMGPVLT"
     gene            complement(10447..10776)
                     /locus_tag="VLA60_00780"
     CDS             complement(10447..10776)
                     /locus_tag="VLA60_00780"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HSF07914.1"
                     /translation="MTSLLLGKIQNTLLTDDRVVREAGPHVMQISVALTEAEASHTVL
                     DTVSSVLPTASLISGTKSLATGTGTFVGSASVEAKITDPELGTLLAAAVNRRGGKIVV
                     GSHIRIE"
     gene            11046..12071
                     /locus_tag="VLA60_00785"
     CDS             11046..12071
                     /locus_tag="VLA60_00785"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSF07915.1"
                     /translation="MKTGAPWPVLLLSVVTGLLLGGPVGVNAADVILLKNGDRVSGTI
                     LLMEDTVLSVDTDYADVIKIDWDDVDGLISEKPLWVAFHDGVTIPDGVGVRDGERLIL
                     FRLDPGGPVQLDKIKTINLFELSYWGNIGLGGSATSGNTTTETINASGTLTVNKGWHR
                     FILDGRANRGKADGEITAQNAALNTRWDYFLSKRAYIPFINFLEYDKFQDLSLRSTNI
                     IGVGYDFLDRRANFLTVAVGPTVVYQNFRFEPSIVIPGFSWQPRWNLEFLGGDLKLWH
                     VHTGTRDIGRDNAVRLNANQGISIKVYQDLSIRFEYNVRYNSKPADGRKTTDTTIIFG
                     LSLDLLG"
     gene            complement(12443..12844)
                     /locus_tag="VLA60_00790"
     CDS             complement(12443..12844)
                     /locus_tag="VLA60_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HSF07916.1"
                     /translation="MQTLKILNVKEIVGILFLLTVTGCTSNVYIDYDTQADFSTYQTY
                     AWAEGTPAKNSLMNHRIIPAIDKQLIGKGFRKVDTNPDMFGSYHAATTEAVSYTTWGT
                     AMVQPAERPMVGTVEDLDCGERVFHLEPPLR"
BASE COUNT         3291 a         3281 c         3540 g         3455 t
ORIGIN      
        1 gtgtttcgaa attgcctcgt gtgtttcatt gcaatcaata atgatttcat cgtgctatgg
       61 cgatgaactg gagccattca atacaagtag ctcaagagga ggaatggcat tgttagaaag
      121 tttatgtagg aacgcgccca attataatcc aaatgaaagg taatatatat gggggaacgt
      181 agattgaaat tttgccattt gaccacatca tcatgcctct gaactgcaga agtcggtcac
      241 tagcctttga tccccgtatg ttgatttttc gtattctctt tttgaatctc gcaatatagc
      301 ttagccgaat gacagggaat aatttctgtg gtcttcaact taagagaagg attcctttaa
      361 agaaaaatgg acactagaaa aggaatgtac aaaaaaattt ttgaatttgg gttttgtcgg
      421 ggattttttt gaaccgggct tggagcgatg gtgggttctg tctgatggga gccctctacg
      481 caatagaatt cagggacaga aaagcactcc tgtctttttg tagcacgtgg agtatgcaat
      541 ttagtagatt gaccaacaga gagcatgacc tgagaaaaaa cctggctccc aatcaggccc
      601 cactaccggc tcttaatcgc ggagtccagc gactgaatga cctcatccag gcggttgatt
      661 atggtgtcct tttgatcaaa gactcggttg gcagtcggtt caatcgtact caatccttcc
      721 ttggttaaat cggatttcag atactgtcta acgtccttga ggtcgttgag aaaaggattc
      781 gcttcattcc tcagcgtcac aattttctgt ttaatatcgg aataggtcgc atccgtgtcc
      841 gcccgtcttt ctgctgcacc cgcccgtgtg gcttcgttat gcaattgttt aagattttca
      901 tcccaagcca tgaattgctc tttcacggca gtctcggtgg cacctcgttg ttgtcgggct
      961 ttctctatcc tgtcttccag gattcccagt tcgtgggtat agctttgaaa ttgcggagac
     1021 aggtctgcct gtggtttgga catcagctca ttgagtgccc caacggtttt ttcaatctgt
     1081 cctctaagcg acaccgcttc atttcgtaaa ccgtcaaggc ctaccgcaat tttgggcggg
     1141 agcgaggcag gagtactgca gctaaccgaa aacatcgtga caactaatcc ggccgcactg
     1201 cttgctagga ttgctctcca ggagggtaca ttcatcccgc tcttgatcat ttgtcactcc
     1261 ttctttaggt tttaagatta tgaaattatg gctgtccgaa ccgactgaca ccgtttggat
     1321 gaggatttta gcgtttttat ttttgatttc atagtggctt ccttctggtt gtcggatttt
     1381 gcaatgactg gttcggcagg taggaggccc catgacggat cggcggttcc ctcttgagcg
     1441 aatcaatgtg cccacgagca cctgcccggc gggccagcgc acccaggagt ttaacccacc
     1501 gtcgtgcccc agtcctcacc cacactgaat gtgtcgtcgg tcggaaattg ccccatactc
     1561 cattttccgt tccaagcgat cattggtgat ggtattgaag aggaagttcg ggcccaaacc
     1621 accaatgagt ttaacgtttg gattcctcag ggccgagaaa tgtcgtatat tcgatgtctc
     1681 ctagccccgg acgacctcct gtattttcct ctatagcatt taccggaaga tggggagggg
     1741 aataattaat cgattgagaa gggcctagtt gccaaatctt ctggtgttcg ttgccttcaa
     1801 attaaaaagg ttcgtaagtt ggtattggcg ccggccccga aaaaggtgcc attggtaaat
     1861 cttgctcgca ccagatcgga gccagggctt ggtacttcct cagccagttg tttgattgcc
     1921 tcggcagggc ttccggaatt tcccatgatg gacaggagca ctcctacaat taaccacctt
     1981 ttgaatgtac tttgagagat cccccaagac tctttggggt taacggaacc tcatgtgatg
     2041 aagcgtatgg aatacatgat taggaaggtt tgtttgtgtc gcaggctcat ttttatgagt
     2101 ctgcaatgag attgggtttt tcggaatcgc atgccgtcat tttctttcca agggccgacg
     2161 ctgtaattag attgcactgt ttggcaacat catgatttgt gatcagggat gagagccagc
     2221 ttttccccgt gtgaaaaatt ttggtatatt ggcgcgcatc cgttacattt tcatttatac
     2281 caaggaggaa gaatccatga agcgattggt gagcggaatc gtgacaatga ccgttctcat
     2341 tgcgttcacg ttgatgctag gcggatatgg ccatgccgaa gaaggcagtg tcaaagcgat
     2401 gtctccgtgg aaagggttag gcttcgcgtt tccggtcggg cctgatcaag tgtatatggt
     2461 ggctgtatac agcggtgtga tgttggtgga tgacggcaaa ggaccgatgc acgcggcctc
     2521 gatcgtttgt cccggcaccg ttgatgctga tttgaaaaag ggaacgaaat caggccatgc
     2581 caagtgtgtc atcacgggtg agcagggcaa tcgtgtcttt gcggaattca attgcaccgg
     2641 cgatgtggaa gggtgccggg gaacgtttac gatcaccggc ggcaccggca ggtttgccgg
     2701 cattacgggg gaaggcgaaa tgatttcgaa gatccaggca cgggaaatta cgatggtcag
     2761 cggtgttgag tcggcccaac aactcggaga gggagtcgcg gtatggccta atttgaccta
     2821 tcatattccc gacaagaatt aaacgcgaaa cgactcattc tctacatgtg gaggcatcga
     2881 tgcgtacgta tttaatcttg gggctgaagt ttggactggg ggtgcttctc cttgcccctc
     2941 ccttgcaagc gcagacgttt gtgttcccgg aaaagggaca gagtccggaa cagcaggagt
     3001 tggacgattt tacgtgttac aagtgggcca agcagcagac cggttacgat ccggagcatc
     3061 cgaacctggc tgccgcgccg ccgcctccat ccggcggtgg tggaatgcgt ggggcgatgg
     3121 ggggggccgc actcggagca attgggggag ccatcgccgg caatgccggt caaggtgcgg
     3181 caatcggtgc tgctgccggc ggcgggctgg gcatgatgcg acaacgtcgg gctgagcggg
     3241 ggtatcagga agaggtgcaa caagcctcgg ccaaaaacca gcagacccgc gccacctttg
     3301 accgtgccca caacgtttgt atggaagcca aagggtacaa gatcgggtag gaggccgtat
     3361 gtccttgatc gaatacataa aggagtagat agatgagggt acagcagtcg tgtattatcc
     3421 tgtcatgcgc ggtcatgctg gcaggttgtg tgggcgctca gcaggtcggg ccagtttcat
     3481 cgtcttatcc tgttgactct gcgcctcctc cgcaggcgaa gacctttgtg tttccggaaa
     3541 agggacaggg tccggagcag caggaacagg acgactttac gtgttacaaa tgggccaaac
     3601 agcaaacggg ttacgatccc gaacatccga acctggccac cgcgcctcct ccgccatcag
     3661 gcggtggtgc gttgttcggc gcgatggggg gcgcggcgct gggagctatc ggaggggcca
     3721 ttgtgggcga tccagggatg ggagcagcca tcggtgccgc tgccggcggc gggctgggtt
     3781 tgatgggaca gcgggcggct gagcaggagt atcagcagga ggtccaacag gccggagccc
     3841 ggaaccaacg cacgcgggag acctttgatc gtgcccatcg tacctgcatg gaggcaaaag
     3901 gatataaggt cggctaaagt gaaaggtttc tgacaaccga ccgggattct tccgcaagaa
     3961 tattcatgca tggtgcgtca tcagctttgg aatgaggcgg tcaaattata cgaagtccag
     4021 actcatccgg accgtcggat gggttgaatg ttaataatgg agcggtgcta ctatggcgca
     4081 ccccacttca tcatctcaac ctacgcactc cctttcagcc gcatggagaa tcacggtttg
     4141 gttccggttc attcctcgat gagccgggcg cctcttactc catgtcattg atgataatgg
     4201 tgtcaggaat aatgaccggt tggcgcgcac tattgcatcg tgcacgttgc catccgtgat
     4261 catgcccaaa ggggggcagg tagccggcat ggtccagtaa caagggcaac tccatcctgc
     4321 ccttgatgcg acaaaattca ttcaggcata gagcgagagg cacagttcaa tcatggtgga
     4381 atccaacctg accggtttat acgggaatca gagcctgcgt atgccgatcc catgagccgc
     4441 gacacgcttc ttgagcctac agaaggtctg agcgaagtgc ctcacgaccg gtggctattg
     4501 gccgaaggca gggacgaacc ggatggtcca ttgaatccgg tggatccaat ccggtcggct
     4561 taaatggcga tactttgcat cgatccttga aagttaacaa tgccgtaaca agctcttgac
     4621 caggccgcaa taagttccag ctaggctagg gctcacatat gggaagaatt ttttcccgtg
     4681 caaccggaat caatctcata ctgtttttgt cagcattgtc agggcttttt gtttaacaca
     4741 aaggaggatg ttcatgagaa aagggattat cgtatttggc atatggggac tcgtgggtct
     4801 gcagattgga tgcacaccat taccaggatc tgatttagtg tcttccaaat cgaacgggac
     4861 aaagatggtg accaccagca cgcagttcga cgaaattgct gataattcgt caagttatct
     4921 ggggcaggaa aagaagctgg caggagcagt ggtgagtatt gatcaaacgg acgaagggta
     4981 tatgatattg gctgaatggc tgccttatcc caaaacgcaa cttgaggaag gacccaaagt
     5041 cgagcaatcc gatcggagta gacattttct tattcgtttt atcggaaaac gggaaaagga
     5101 attttacacc actcggggaa atatctttct tttagaagga acagttgaag gaacaaaaaa
     5161 agcactggtg aatgtcttcg gtccgcgaaa agatctactc tatgtcaatg cgaaatgtgt
     5221 ccgtatctgg gaaaccggac aagacactga ccgctccagc caattggaca acccgtatcc
     5281 tccgacgaga aatcgaacgc tctgtgcgga ttaaaatgag aatccacata ggtaataaat
     5341 ggtaccattg cttcacgcaa gcgtcggcaa ttggtcatcc gcatacggtg ggtaggaatt
     5401 ctcctagcaa tctttgcatc cggggccctg catgcagggc cccaatcctg acaagccgaa
     5461 gtaaccaact cctccaatcg ccgactggag gctgaatgga aagaagcagg aaattcattc
     5521 aaccaggtct gcaagcatct tggcttagtg tttgaagtca aacggttcac tcgacatatt
     5581 cacgtgtcca accgtgactt tcaaggcatt tctccctcat catctctcct gcccctcttt
     5641 cgtagcgttt ttgcctgctg gtgactgtga cttcaccatc cgggtccaat gtcctcatat
     5701 taccccgccc aaaatgaata ttctcacgat tacaaagcgt aatagccgct tgcaggtctg
     5761 actctgtcgc gccaggcttg acccactgca gtgttgggcc acaagctgac agcagagcaa
     5821 aaaccaccca ccataatcgt ccgttcatct ttttaacccc ttcttgattg ttaatcggga
     5881 tgttaaattt gcctgtagca ttgttatgtt ttcttcaagg tgattccgtg tggggtatat
     5941 tgttgggtct ttcatcatca ggaaagaggc aatcaagtac caaatactag aacgtcatgt
     6001 gaaggttccc atcggatacc ttgagaatgc gatctaacac ggaaactcgc cagggccgtt
     6061 taggcgggtt tatctgggaa caggatttga aactggagtt gtagcgtcca atcaggcgtc
     6121 agatccggtt tttccaggaa ataaaatccc tggagtttga tattgatggg ccgtttttct
     6181 ccgcggaaca tgactttccc gaatcctcct ccaaggggca ccgtccagcg attccgcttg
     6241 tcctccgcct cccaattcgc aatgatcgcg ggggtagaag tcaggtacca gccattggga
     6301 aaattgtaat tgaggaaggg ttgaatcaca aactggttaa ccttgaggcg ctgattatca
     6361 cccgcaaagg accagatatt tcggatgagg gcacccatca cccagggatc cggtatgctc
     6421 acgagcgcca gagtgggtcc gacgctccat ttcccgcgtc ccaagcggtc atcggtggcg
     6481 gtattaagaa tgaaagtcgg tccgaaaccg gcgattaact tcagcagtcg cttggattcg
     6541 tcacgggcta gaaacggcgt aaactcgata tctcccaacc cggtaagact tccggattta
     6601 tcccctatgg cattggcggg aaaataaagg atggggatag ttagccggtt gagaagagcc
     6661 cattgaccaa atcttctggt gttacccgcc tccagattga agacgttcga gagatgattg
     6721 ttgaatccgg tcccaaagag ggtgctgtta gtaaacccaa cccgcacaag gtcggacacg
     6781 ggattttgta cttcctcagc cagtttttta atatcctccg catgggctgt ttgactcccc
     6841 ataaagccca ggaggatacc tgcaatgacc cagcttttgt ttattttctg agaagccaag
     6901 aacattcctt ttgcacacct aagcctcggt tgaggtttag tgaacataac atcagagtaa
     6961 aacaggagga acaggagcaa tttgttgttg tctagtttag caggactcgt ggaagattat
     7021 ctctcatcag agagctgatc aagaacggag gaacctctct cttctcggtt gcgtttttct
     7081 cctttcttgt gaaaacagaa aaccggatgg atggccgata aaaatgtcgg acctgactga
     7141 cttttttgtc attcccgccg gttagcggca ttcgactcga acatgccaaa aaggattcac
     7201 gaaaaattga tggactaagg aggacaagaa attttatgta cccacgggaa tagcagatgg
     7261 aaggtttcct actcgcatgc accatgtaca ccatccctca gacgcttccc gaaataaaaa
     7321 gtttcagtta taacaaccgg gcacggcttt cgcttgttat ttcggttgat atccacttaa
     7381 taaagtattg gattctatct caaaaatatt cataataata tgttggaaat tgtacaaggt
     7441 tgactcgacg tttatgacca tgatgggttc aaagcgacaa aagatttggt gtctctcttg
     7501 tcttgtgctg gtgacatctg tcctgtttct ctccttggcg ctgaatcaga agcttctcag
     7561 gagttggagg atgacccatt cagtgcgaag ttccgtcaga ccctgaaccc atggacaggt
     7621 gattgggacg ggatggtcac acgcaaccag gttcgtctgc ttgttccctt tagcaaaaca
     7681 ttttactttc tggacggcgg acgccaacgt gggctcacct atgatcttat gggcacctct
     7741 aaaaacgttc gagtaagctt gtttgccccg tggtggagag gccaggcacg aaatccggga
     7801 gcgaaaagag aagggagacg gatctgcgtc cgtgcgcttg tcttttcatc atcgttcccc
     7861 gtccccttcg ttacgccgtt tgctctctgc cagcggccgt cagcaccaag aatcgctgga
     7921 agttgtcggt cagattcttc agcccgatct tcgcccgcgc tcggacccgc ccaatggttc
     7981 gaaggagttt ccctcccatg gccgtgacgt gatggccaaa gacatgttcc acgcgagcgc
     8041 ggatccggct ccgacgctga ttcgtttgct gttgtttggg cgtcaacggc ttgtcgcgat
     8101 agcccctgta ctgaatctgg gaacgcagct tccgcgtctt gagtttgcct tcaatctcgt
     8161 ccgaccgata ggcggcatcc gcccatacgt cccgcccagc ggtcttgggc tgcaacacgt
     8221 cttccaggac ttgactgtca tgcaccgccg catccgtgac ggtatactgc cgaatgagtt
     8281 tgtgctgttt atccacattg acatgatttt tatacccaaa gtgactgacc ccatgttttt
     8341 tggtccagcg ggcgtcggta tccttctgcc gccgtttcgc cggctgggac tcccagtctg
     8401 ctgggcactc gccagcctgc accgtcgcat tttcctcccg ggtattgcgc tgcttgggca
     8461 cggggaccag cgaggcatca atgagttgcc caccacgagc ttgcaagccc tggttggcca
     8521 gataggtgtc aaattgggca aagaggggct ccacgacgcc ggcttgagcc aacgtctcgc
     8581 ggaacaacca gatggtcttg gcatcgggga tccgctgatg cagatcgagg cctaagaagc
     8641 gcataaacga aagccgatcc cgaatctgat attccgtttg gtcatcggat aagttataga
     8701 gagcttgcag cacgaggact ttgaacatga gcagggcatc aaacggcggc cggcctccct
     8761 tcgaacgtgt cgagcgctgc aggcctttct ccagcgtctt ccgaaagcgg acccagggga
     8821 tgcgctgagc gagttgtact aacgggtcac ccttctgggt gagagcgtca tagcgatgag
     8881 tcaggtcaaa gaacccaagt tggccagatg tgtccatgag caaacctcct tggcggagac
     8941 ttatgccaga atcgaacaaa tatgtcagca gtttttagag gtgcccttat gaaaatattt
     9001 gaaaaacaga ttaacgcaga tttgaaacgg aagaccgtgc aggttcagtt cgttttcatt
     9061 cctgtcaacc gagatgaatt tattccgaat taactcaatg gaataggaga tatccccgct
     9121 ggagatctta cgattacacc cgaaagtcaa caactcgttg atttttccga accgtttctc
     9181 accaacgttc gtgagattat cgtaaccggt cctggcagcc ccactttctc cagtctggat
     9241 gatctttccg gacacaccat tcatgtgcgg aagtccagta gtttttacaa gcatcttgtt
     9301 cgattgaatg cctcgtctaa attagccgga caaccggaga tcacactcgt ccctgaggag
     9361 gagaaccttg aagacgagga cctcctcgaa atggtgaacg cagggctatt accaatgctg
     9421 gtgatggatg cccctcttgg cgaattttgg gcaaaaattt tcaagaatat ccgcctgcat
     9481 cccgacattg ccgttaatgc aaacgggaag atcgcttgga cgttcaggaa aaacagccag
     9541 aagctggcga aggtgatcaa tcagtttgca aggaacaata agaaaggtag cttaattgga
     9601 aatatgttat tcacccggta tttacaaaac agcccctatg tcaaaaatgc cctggccact
     9661 caggaacgga aaaaaatttc aacgtctcat gcatgtgttt gaaaagttcg ccaagccata
     9721 tggatttgat tgggtgcaca ctatggctct aggctaccaa gaatccatgt tagaccaacg
     9781 caagcgaagt ttggcgggtg caatcggcgt gatgcacctc ctgcccagtt ccgcccggga
     9841 tcctaacgtg agtattccca atattaataa gcttgagccc aatacacatg ccggagtgaa
     9901 gtatttacat tttctgcatg accggtattt taaagacccg catatggatc tgttgaatca
     9961 atggcttttt cgatagcctc atataatgcc ggacctgcca aaatcgcgaa gctacggacg
    10021 gcggcggcca tgggcatgaa ttcgaataag tggtttaaaa atgtggagtt ggtcgcggca
    10081 aagcgtatcg gtcgagagac tgtccaatat gtgagtaata tttataaata ttttatggcg
    10141 tatcgcctga ttctagaaaa agaggccata aaattagcgg tgtattaagc cctctgagga
    10201 gccctaataa aaataaacct tccttgaatg accggagagc agcgatgacc accttcgagt
    10261 ccaggcaacg ggttactcag catgcagaag tgatcacagg gatgccgcct gggtatgagg
    10321 gattgggaga gtattccagg cgttgtcgat tcttatcccg tgggttgctc acaactgtgt
    10381 tccccgcgcc attggctcag gcgatagcgg atagtatggg ccccgtactg acatgattct
    10441 tcgacgtcat tcaattctga tgtgactccc gacaacaatt ttgccccccc ggcgatttac
    10501 tgcagcggcc aaaagagtcc caagttctgg atcggtaatt ttggcttcta cgctggcgct
    10561 gccaacaaac gttccggttc cggttgcgag agattttgtg ccggaaatga gagatgcggt
    10621 cggaagaaca ctggaaacgg tatctaacac ggtatgtgag gcttcggctt ctgtgagtgc
    10681 cacactaatt tgcatgacat gaggtcctgc ctcacgaacg acccggtcat ccgtcaataa
    10741 agtattctgg attttgccca agaggagcga agtcaaccga atgcgatctt cctagtggat
    10801 atcccgaaga gaagatccct ctcccatcca gagttggatt gggtcaatca tcaccttggt
    10861 atattgattc cagtctgcaa agggaggtga acaaaccatc ccactcttcc ggaaacgaca
    10921 ctcatgaatg gggtaggggg gttaccttta ccctgggagt cacggaatct atactgcctt
    10981 gcacgcctat gaccctttcc caccatttca tccctcactg gtgtaactgg ttcctgaggc
    11041 cgcgcgtgaa gactggcgcg ccgtggccgg ttcttctctt gtctgtcgtg acaggattgc
    11101 tgctaggagg acccgtggga gtgaacgccg cggatgtcat tctactcaag aacggagacc
    11161 gcgttagtgg aacgattctt ctcatggaag acacggtcct cagcgttgat acagattacg
    11221 ccgacgtcat caaaatcgac tgggatgatg tcgacggact gatatccgaa aaacccttat
    11281 gggtggcctt tcatgacggg gtcacgattc ctgatggcgt gggggttcgg gacggggagc
    11341 gcttgattct ctttcggctc gacccgggtg gtccggttca actggacaaa atcaagacga
    11401 tcaatctgtt tgaattgtcc tactggggga acattggact tggggggagt gccacctcag
    11461 ggaatacgac caccgaaacc atcaatgcct cgggaacgtt gaccgtgaac aaaggctggc
    11521 atcgatttat cctggatggg cgtgcgaatc gcggcaaggc cgatggagaa atcaccgcac
    11581 aaaatgcggc attgaatacc cgctgggact acttcctgtc caaacgggcc tatatcccgt
    11641 ttattaattt cctggagtat gacaaatttc aagacctgtc tcttcgaagc acgaacatca
    11701 tcggggttgg ttatgatttc ctggatcgcc gggcaaattt cttgacggtc gccgtgggcc
    11761 cgacggtggt gtatcaaaat ttcaggtttg aaccgagcat agtcatcccg gggttttcat
    11821 ggcaaccccg ctggaatttg gaatttctgg gtggcgatct gaagctctgg catgttcata
    11881 ccggtacccg ggatatcggg cgggataatg cggtgcggct gaatgctaat caaggcatca
    11941 gtatcaaagt ctaccaagac ctctcaattc gtttcgaata caatgtgcgt tataattcca
    12001 agccagccga cggtcggaaa accaccgata ccaccattat ctttgggttg agtcttgacc
    12061 tattgggata ggggggaaga cgccatggag cggtgtgctg aggatctgaa agtctttgcc
    12121 aggaaaccgg tgataggtct tttgtggagt aagcccatca ttcctatttt cgggtcatta
    12181 ttcgatccaa aactgatgat ggattgaatg ggaatgaagc gatgcgaggg gaattcacct
    12241 atggaataag tcgttgaacc ttgtgtgcca tatcacttgg caggcgaagg gaatttggcg
    12301 aacatctctg tggtggctgt atgaatctga tcctcaacct tttcagggtc ggcatgaacc
    12361 gtgtcattcg cggtgccccg ctacagcaat gattttttct cggcatcgaa aatgtctacg
    12421 accagcgtgc ctttgactac cgttaccgga gtggcggttc cagatgaaag acccgctccc
    12481 cacaatccaa gtcctccacc gtaccgacca taggtcgttc cgccggctgg accatagccg
    12541 taccccatgt ggtatagctc actgcctccg tcgtggccgc atggtaggag ccaaacatgt
    12601 ccggattggt gtccaccttc cggaatcctt ttccgatcag ttgtttatcg atagccggga
    12661 tgatccggtg attcattagt gagttttttg cgggtgttcc ttctgcccac gcataggttt
    12721 gatatgtgct aaagtctgct tgggtatcgt aatcgatgta cacgttggat gtgcagcccg
    12781 tcactgtcag gagaaaaaga atgccgacga tttctttgac attcaaaatt tttaatgtct
    12841 gcatgccttg ttccccattt ttcggaaata cccaaacacc aatatccccg ccattttgat
    12901 gtatcggtgc gaatacatct tctgtacgca tgagtgtgga agctctggaa aaacgacggc
    12961 ggatgttgcc atacatgcgt cggtcggttc aatcgtcgca ttattttaaa tggcagaaaa
    13021 cgagaactga tgtcttctcc agataatatt tttcactcct cccattttct tcgctcccat
    13081 tctataaaaa tctagctgaa ccgggtgacc tcatcggtgg attcgccccc cgaagttctg
    13141 cgttcttttc ggccgggtat ttcggaaggt aaagcacctg ctgctcctgc cactccacgc
    13201 ctcgcgccaa ggtggtaaac gtcaatgtag catcctcgcc ttttccagga ttagttcccg
    13261 gaccttgcat tccttcccgt cccgatggag caggtcgcgg gcaagttcct gacgacccgt
    13321 tttatccaat caaatgcctc ctcaaaaact acaacccgaa ttgcacagat catttccgtc
    13381 tctttttcct gtatctataa ctagggttta tttatgtagt agtgtaaaaa aaaatatttt
    13441 agtgtatgca gtcaaatttt gacgaaacgt catttatcgt gctataaaat aacccatggc
    13501 cacctcagaa agaaaaaaac gagagtatgc acagcgggaa acgctgatta tcgacaccgc
    13561 gagacac
//
LOCUS       DATIAL010000023         9778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_971772, whole genome shotgun sequence.
ACCESSION   DATIAL010000023 DATIAL010000000
VERSION     DATIAL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971772"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VLA60_00795"
     CDS             complement(<1..1302)
                     /locus_tag="VLA60_00795"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07917.1"
                     /translation="MLADVVLPARRFQIFTYQVPPHLLSLLHVGSPVLVPLGSTVVSG
                     VVVTLFETQNSSSLQTQFRQKPLRAILSLEADAGNRPLEHNLLRLVEKISDYYVAPFS
                     ACLRLIVPPHSIPVIRRISLTDDGRQALSDRSLAPDVQLVLRKLEQAPRGLLRSSLMR
                     TMNNVSDILTRAKKKGWIFEQTTLPSGSKGQSPVRVIRVERNPLHSVPRGLFDSPEES
                     REIPESVQRRAFSFKDNRTWNILFTAVQAGGFQEVPVVGSESVQLELLFKMIETILEK
                     GCRVLILAPEVQQAEILGKQIRLLGNIQVEVYHGHLSTMVRAARWERIRQGDVQVVVG
                     TRSALFLPVPNLGLVWINQEEDPSYKDEHLPYFHAREVARMRGECDQALVVYGSTCPS
                     LELYGRFTEQIGQALECPSQQIPHVNTVDLRTLPYETILSPA"
     gene            complement(1882..3270)
                     /locus_tag="VLA60_00800"
     CDS             complement(1882..3270)
                     /locus_tag="VLA60_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF07918.1"
                     /translation="MRANIFVTDDDDVVRQAISRRIAKRHHHVRSFASGEALLEALDH
                     DVPDLILLDLKMAGLSGLETLKHIRAKSQQSLVILLTAYGTVEDAVEAIKLGAYDFLI
                     KSVDFSSVEPVIDRALDYLTLRRRIDFETQDRPGRYAFNSLIANSPSMKELVGQIQEM
                     AKNLKTTVLLFGETGTGKEFVARVLHHNGPRDKGPFVGVNCTAIPQELFESELFGYER
                     GAFTGANQRKPGLCEQAEGGTLFLDEIGDLNPSMQAKLLRVLQERSFKRLGGQEDIDV
                     DFRLIAATNRDLRKDVAQGRFREDLFFRLNVVSLNLPPLRNRVEDIIPLALATLIRQS
                     SDLGKEVSAIEPEAQRLLERYPYPGNIRELENIMERAAIFCRGKSLTEGDLPREVHEE
                     ARSSVNAATRGDQKVVRMEMILGEQTLAGIETDLIEEVMRLSDYNKSLAAKYLGITRF
                     ALDRRLKKIPES"
     gene            complement(3274..5199)
                     /locus_tag="VLA60_00805"
     CDS             complement(3274..5199)
                     /locus_tag="VLA60_00805"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF07919.1"
                     /translation="MSLNKLFLNLPIARKLLLVSGVPVLAVLILSLVTYRSVQTFSLD
                     EDRLNDVYLVQSASAEYMRLVVDLETGFRGFILTQKAPFLQPYLAARQRVLLLGNSLR
                     QMVKSVEDQRQVIETVQGMVEQLMNDKDRLIERVKAGFPQEATDYVESEKGRILMLAI
                     REEMARFDRREVTQLQHALGSSAADRAALMGVVVWGGSLALILLLVPLHLIARSITGP
                     LTTLVKAVENVSGGNIPNIPVFQRDDEIGELTKVMQTMGTQIRNHIRRIEQSEQELRT
                     LNQDLTSSEAKYRGIVDYAPIGIFTAKKDHLIFSNRQNWVLAGRNPDEVLDPEHMWEA
                     IHPDDREGVRETFQDACHQSEPFERVLRFLHPDGAVRKVLCRAVPIQDHGGQTMVYQG
                     FNVDITALEHMRAQLNRSDRLATLGQMAAGIAHEIRNPLVGIGSTTTLLMEDFPDGDS
                     RHEDLVTILKETRRLDRIVNQIVDFARPRDLLPVTFQLQDLILESLQVLNEPIKQKAI
                     QIEFHPPANLNSIQADRDQLKQVLLNVIQNAVEAMSEGGCLRLSVEEETIERIPGLRI
                     KVKDNGKGISPSALPRIFEPFFTTGKHRGTGLGLPICRNIIEAHGGEIHAESQSGIGT
                     TMTWWLPCVCQPQLPTV"
     gene            complement(5529..7148)
                     /gene="nhaB"
                     /locus_tag="VLA60_00810"
     CDS             complement(5529..7148)
                     /gene="nhaB"
                     /locus_tag="VLA60_00810"
                     /inference="COORDINATES: protein motif:HMM:NF007093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HSF07920.1"
                     /translation="MLPFIRAFEQNFLDHAPRWYKGTILAFLIVNPCLLYAVGPFVTG
                     WCLVLEFIFTLSMALKSYPLQPGGLLALEAVLIGLAKPATVYREALNNFQVILLLIFM
                     VAGIYFMKDLLLFVFAKVLLEVRSKTTLALLFAGMGAFLSAFLDALTVTAVIIAVGVG
                     FFKVYHRVASGKGIQDDYDHTMDHGVGTLHRSNLEEFRGFLRNLLMHGAVGTALGGVC
                     TLVGEPQNLLIGEKAGWNFIEFFIRMSPVTIPVLIVGFLTCVLVERFRWFGYGFSLPP
                     VVRDILWRFDQETSRKMDDRFKARLIVQGLAMIWLISALAFHLAEVGIIGLSVIVGVT
                     AFNGIIEEHRLGEAFQEALPFTALLVVFFSFVAVIQEQQLFGGIIHYVLSMDGGHQIG
                     MFFIANGLLSAISDNVFVATIYIEQVLRAFHQGVISRDQFDLLAVAINTGTNIPSVAT
                     PNGQAAFLFLLTSALAPLIRLSYGRMVWMALPYTVTMTLGGLLAVYVLLQPVTETYYA
                     QGILTHHVAAVAEPHPAQGIPASMVGSTFYK"
     gene            complement(7200..8999)
                     /locus_tag="VLA60_00815"
     CDS             complement(7200..8999)
                     /locus_tag="VLA60_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF07921.1"
                     /translation="MEHLTAGIRIYLKDFNFKENFKGIASNVRGDVLAGITVAMVVLP
                     MALAFGVASGLGAIAGMWSAVAAGLIAGPLSGSAWSVGGPTGPMTIQILSMTQTNKLA
                     DGSPDLVFVFTTIALAGFILIALGLLKLGQFIKFTPYSVISGFMTGLGVLYMLLQLNP
                     FLGLPGVKSITSAITELPSSLVHASPVALGIGLLTLLIVIVWPKISPVTWLPGPLIGL
                     LVGTVTTVVLGLDIPKIGDIPTGLPELYLPNLDILGKAFVPAAALAGLCIFDSLLTCL
                     IVDNMTGTHHNSDRELVAQGSANLFSGLVGGLGGATNTMPCVVNIQSGARTRLSSITM
                     GFVLLSFIFGLGSLAASIPLSALAGILLKAGYDILDMRVLPVVRRLPTSDLMVFGLVV
                     LMTVFWNLLSAMAMGLAVAFFRFVKDQSDRYKADLSQRDEDVKQEEEDLIFSFARDYA
                     RKISQNGANMGELSGRFEHIVRDRIIIVRPHGPLFFGAIDWLNETVEHLDSKDVLIIR
                     CKWLDELDLSGAYALGDLIEAANSRGVAVLTAGMSPRTRQVLADLNELSRLKEDYICT
                     HFNEALDTAMQIVEKKAVEVRQQAPKKPVVTVS"
     gene            complement(9355..>9778)
                     /locus_tag="VLA60_00820"
     CDS             complement(9355..>9778)
                     /locus_tag="VLA60_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07922.1"
                     /translation="RNPKKNFDFLFAKRRAEYGPTGILGIIFPSNIFSPIGFRSTLQP
                     ISTVRLVASYRAFWLADGRGPFVGSGLQDPTGRAGTFLGNMLDSSITWAPQAGYFRHT
                     TFEVGNTRFFKGSYFDRVPQSPGTADVNYVYAMATLTF"
BASE COUNT         2759 a         2594 c         2199 g         2226 t
ORIGIN      
        1 agccggggac aggatggtct catacggtag ggttcgtaaa tcgaccgtat tcacatgggg
       61 aatctgctgt gatgggcatt ccaatgcctg accgatctgc tctgtaaaac gtccatagag
      121 ctccaaggaa gggcacgttg aaccatatac tacgagagcc tggtcacatt ctccccgcat
      181 tcgcgcgact tctcgagcgt gaaaatacgg aaggtgctca tctttgtagg acggatcctc
      241 ctcctgattg atccaaacga gccctaaatt tggaaccggc agaaatagtg ctgatcgagt
      301 ccccaccacg acctgcacgt cgccctgtcg aattcgttcc catcgagcag cacgaaccat
      361 tgtcgaaaga tggccatgat agacctcaac ctgaatattg ccgagaagcc gtatctgttt
      421 accaaggatt tcggcttgct gcacttctgg agcaaggatg aggacgcgac agcctttttc
      481 cagaatggtc tcaatcatct tgaacaacag ctccagttga actgattccg atcccacaac
      541 aggcacctcc tgaaaaccgc cggcttggac tgcagtgaac aaaatgttcc atgtccggtt
      601 gtccttaaag ctgaacgctc ttcgttgaac agactccggg atttcccttg attcttctgg
      661 agaatcaaat aaccctcggg gaactgaatg caacggattt cgttcaactc tgattacacg
      721 aaccggactt tgacccttgg atcctgaagg cagggtcgtc tgttcaaaaa tccaaccctt
      781 ctttttcgca cgagtcaaaa tatctgaaac attgttcatc gttcgcataa gagaggatcg
      841 gagcaatccc ctgggagcct gttctaactt gcgaagaacc aactggacgt caggtgccag
      901 cgagcggtcc gataaggcct gtcgaccgtc gtccgttaag gatattcgcc ttattaccgg
      961 tatcgaatgg ggggggacaa tcaaccgcaa gcatgcggag aaaggagcca cataataatc
     1021 tgaaattttt tcaaccagcc gaagaaggtt gtgttccaac ggacggttcc cggcatcggc
     1081 ctccaatgaa agaatggcac gtaacggttt ttgacggaac tgcgtttgca gggaagaaga
     1141 gttttgtgtt tcaaagagcg tcacgacgac tccagagacg acagtagagc ctaaaggcac
     1201 caacacgggg ctccctacat gcaacaggga aagcagatgg ggggggactt gataggtaaa
     1261 gatctggaac cgtctggccg gcaggacaac gtcagcaagc atgagtgatt acggttttca
     1321 tgagcacaca attctatcag cgagttgttc tgcctacaac gctattccac ctcagccctc
     1381 agatcaacaa aatatattgt gggccacaag gaggtaagca ctgagtctat aaaaagattc
     1441 caaccgactt gctcgctcat cctcatgatc aatgcccagc atgcgaggta gaagaaaatc
     1501 aggtttgata cggcaccttg acgatttcga taaaaacagg aaagatagaa acgaccggct
     1561 gatatcctat ccagccggtt aggcaagaat cggtcagttt acaggctttt gcccctacaa
     1621 cataccgcag gagagataaa taccgaagcc gcgcgatcgg aggaggatag caaaatggga
     1681 ggcttggaaa cacttggaat cttggctcta ttgattggaa tcattggcat cgttgttctt
     1741 gtaaaaagtc gaaaaaaatc ccaataagga cagggcaatt ccccctgacc acctccagac
     1801 atcaacttgt tctcttgttc tgaggagacg aacatacgaa cattttcatt tggtgattat
     1861 ttcaagcacc catagatagg atcaagactc ggggattttt ttgagtcggc ggtccaaagc
     1921 aaagcgagta atgccaagat atttggcagc gagactttta ttataatcag ataaccgcat
     1981 gacttcttca atcaaatcgg tttcaatgcc cgcaagggtt tgctctccga gaatcatttc
     2041 catgcgcacc accttttgat cccctcgcgt ggccgcattt actgaagaac gagcctcttc
     2101 atggacttca cgaggcaaat ccccttccgt caaacttttt ccccgacaaa aaatagccgc
     2161 acgctccata atattttcca gttcacggat attccccgga tagggatagc gctccaacag
     2221 ccgttgggcc tccggctcaa tagccgaaac ctctttgcca aggtcgctgc tttgtcgaat
     2281 caacgtcgca agagccagag gaataatatc ctctacccga ttccgtaatg gtgggagatt
     2341 caatgacacc acattcaacc gaaaaaataa gtcctcccgg aatctgcctt gcgccacatc
     2401 tttgcgcaga tctcgattgg tggccgcaat cagacgaaaa tccacatcaa tatcctcttg
     2461 cccacccagc cgtttaaacg aacgctcctg caacactcga agcaatttgg cctgcatgga
     2521 aggattgagg tctccaatct catctaaaaa gagcgtcccg ccttcggcct gttcacagag
     2581 accgggtttg cgctggttcg cccccgtgaa agccccgcgc tcatagccaa ataattcact
     2641 ttcaaataat tcctgaggaa tggccgtaca attcaccccc acaaacggac ctttgtcccg
     2701 agggccgtta tgatgaagta ctcgggcgac aaattctttc cctgttcccg tttctccaaa
     2761 caggagaacc gtcgttttca aattttttgc catttcctga atttgcccaa ccaactcttt
     2821 catactggga ctattggcaa tcaaactgtt gaaggcatat cgaccgggcc tatcctgcgt
     2881 ttcaaaatcg atacggcgcc gaagggttag atagtcaagc gcacgatcga tcacaggttc
     2941 cacactggag aaatcgacgc ttttaattag aaaatcatag gccccgagtt taatggcttc
     3001 caccgcatcc tccaccgtgc cataggccgt gaggagaatg accagggact gttgagattt
     3061 cgcacgaata tgcttgagag tttccagtcc gcttaaccca gccattttca aatctaacag
     3121 aatgagatct ggcacatcat gatcaagggc ttccaataag gcctcccctg aagcaaagct
     3181 tcgaacatga tggtgtcgtt ttgcaattcg tcggctgatg gcttggcgaa ccacatcatc
     3241 atcatccgta acaaaaatgt tcgcgcgcat gggttacacc gttggaagtt ggggttggca
     3301 aacgcaaggc agccaccatg tcatggtggt cccaatacct gactgacttt ccgcatgaat
     3361 ctctccacca tgggcttcaa taatatttcg acaaataggc agtcccagtc cggttccacg
     3421 atgcttgccg gtggtaaaaa acggttcaaa aatccgaggg agagcacttg gactgattcc
     3481 ctttccgtta tctttgactt taatacgcag tcccggtatt cgttcaatag tttcctcttc
     3541 aacagacaga cgaagacacc ctccctccga catcgcttca acggcatttt ggatcacatt
     3601 tagcaacact tgcttcagtt gatctcgatc agcttggatg gaattcagat tggcaggcgg
     3661 gtgaaattcg atctgaatgg ccttttgttt tatgggttca ttgaggacct gcaaagactc
     3721 cagtattaga tcttgcaatt gaaaggtcac cggaagcaag tctcttggtc gcgcaaagtc
     3781 aacaatttgg ttcacaatgc gatccagtcg ccgggtttcc ttgagaatgg tcacaaggtc
     3841 ctcatggcgg gaatcaccat caggaaaatc ttccatcaac aacgtcgtgg ttgacccaat
     3901 acccacaagt ggatttcgaa tctcatgggc aatacctgcg gccatttgtc ccaatgtggc
     3961 caatcggtca gatcggttca attgtgcacg catatgctct aaagccgtga tatccacatt
     4021 gaatccctgg tacaccatgg tctgcccacc gtggtcttga atcggaaccg cgcgacataa
     4081 gaccttccga acagctccgt ccggatgcag aaagcgaagg acccgctcaa agggctcact
     4141 ctggtggcag gcatcttgaa aagtttcgcg caccccctct cgatcgtccg gatgaatcgc
     4201 ttcccacata tgttcaggat ctaacacctc atcgggattc ctcccagcca gaacccaatt
     4261 ctgccggttg ctaaaaatca gatgatcttt ttttgccgtg aatattccta ttggcgcata
     4321 atccacaatc cctcgatatt tggcctcaga agaggttaaa tcctggttca gcgtccggag
     4381 ttcctgttct gattgttcaa tacgccggat atgattccgg atttgggtgc ccatggtctg
     4441 catgactttc gtcaattcgc caatttcatc gtcccgttga aaaacgggaa tattcggaat
     4501 gttccccccg gaaacatttt ccacggcctt gaccaaggtc gttaatggtc ctgtaatgga
     4561 ccgcgcaatg agatgaagag gcaccagcaa caaaatcaac gccaacgacc ctccccacac
     4621 gaccaccccc atcagagccg cccggtctgc ggcgctactc ccaagggcat gttgcaattg
     4681 cgtgacttcc cgtcgatcaa accgagccat ttcttcccga atggcaagca tgaggatacg
     4741 ccctttttca ctctcgacat agtccgtggc ctcctgcgga aaacccgctt tgacccgttc
     4801 aatcagccga tctttgtcat tcattaattg ttcgaccatt ccttgaacag tttcgattac
     4861 ctgtcgctga tcctcaacag acttgaccat ctgtctcaat gaattcccga gtaacagcac
     4921 tcgttgcctg gctgccaaat agggctgcaa aaatggcgct ttttgggtca gaataaaccc
     4981 tcgaaacccg gtttcgagat ccacaactaa tcgcatatat tcagccgacg cgctttgcac
     5041 cagatacaca tcattcaggc gatcttcatc cagggaaaaa gtctgaacgc ttctataggt
     5101 caccaggctc aaaatcaaga cggccaaaac cgggacccca gagactaaaa gaagctttct
     5161 ggcaattgga agatttaaaa ataatttatt gagagacata gcgaattaac ccaaactctt
     5221 ctggagtccc ttcaaacctg cccctgacgc ctgacataac acatccagac cctcgcccca
     5281 ctatacccca ataaccggtc taatctcatt atgtgaggtg cccaaaccag gatcatttct
     5341 ccactcagag tctttttctg gaacagattg accggatggt gcgaccccag ctaacggaga
     5401 aagattccat ggtcaaataa gaacagtccg tgatcacagg cggaaataaa tggccggcaa
     5461 agccgcatgg tgaagatcgg ggagcctgca agcatggggg agaggtaaga ggtagacgca
     5521 cccaggactt acttgtaaaa ggttgaacca accatagatg cgggaatccc ttgagctgga
     5581 tggggttctg caacagccgc cacatgatgt gtcaagatcc cttgtgcata gtacgtttcc
     5641 gttactggtt gcaacaacac atacacagcc aggagtcccc caagggtcat ggtgaccgta
     5701 tagggaagag ccatccacac catccggcca tacgaaaggc gaataagtgg ggcgagagcg
     5761 gacgtcaaca gaaaaagaaa ggccgcctgc ccatttggtg tggccacact aggaatattg
     5821 gtccccgtgt taatagcgac cgccaaaaga tcaaattgat cacgggaaat cactccctgg
     5881 tgaaatgccc gcagcacctg ttctatatag atcgtggcca caaagacgtt gtcgctaata
     5941 gcggaaagaa ggccattggc aataaaaaac atgccgattt ggtgtccacc atccatggac
     6001 aaaacataat ggataatgcc tccgaaaagt tgttgctcct ggatcaccgc cacaaacgaa
     6061 aaaaatacta caaggagggc cgtaaacggg agagcctctt ggaatgcctc tcccaaccga
     6121 tgctcctcaa taatgccatt gaaggccgtc actcccacaa tgacggatag tccaataatg
     6181 cccacttccg ccaaatgaaa agccagggcg gaaattagcc agatcatagc cagaccctga
     6241 acaatcaacc gggccttaaa ccgatcatcc atcttacgtg aggtctcctg gtcaaaccgc
     6301 caaagaatat ccctgaccac gggaggaaga ctgaatccat acccaaacca tcggaatcgt
     6361 tcgaccagga cacaggtcaa gaatcccaca atcaacaccg ggatggtcac aggagacatg
     6421 cgaataaaga attcaataaa attccatccc gccttctctc cgatcaacaa attttggggt
     6481 tctccgacta aggtgcagac acctcccagc gcagttccaa ccgctccgtg cataagaaga
     6541 tttcgtaaaa accctcgaaa ctcctctaaa ttcgagcgat ggagggtgcc aaccccatga
     6601 tccattgtat gatcataatc atcctgaatc ccttttcctg aagccacccg atgatacact
     6661 ttgaaaaacc cgacgcccac ggcaatgatg actgccgtca cggtgagggc atccagaaat
     6721 gccgacaaaa acgctcccat tcccgcaaat aacaaagcca gggtagtctt cgatctgacc
     6781 tcgagcagga ctttcgcaaa aacaaacaac aataaatcct tcatgaagta aatcccggcc
     6841 accatgaaaa tcagcaggag aatgacctga aaattattta atgcttcgcg gtagacggtt
     6901 gctggttttg ccagtccgat cagcaccgct tcaagggcca ataaaccacc tggttggaga
     6961 ggataacttt tcaacgccat ggacaaggtg aaaataaatt caagaacgag gcaccagcct
     7021 gtgacaaagg ggccaacggc atacaataaa cagggattaa ctatgaggaa agcgagaatc
     7081 gttcccttgt accaccttgg agcatggtcg agaaaatttt gttcaaatgc acgaataaac
     7141 ggtaacaccc taatactaaa tctccacaaa aatgattcac aagcgagtgg tgtaaaaatc
     7201 taacttaccg ttacaaccgg tttttttggg gcttgttgcc tcacctccac ggccttcttt
     7261 tccacgatct gcatagcggt atccaaagcc tcattaaaat gtgtgcagat atagtcttcc
     7321 ttcagtcttg aaagctcatt taaatcggca agaacctgtc gggttcgagg ggacatgccg
     7381 gcggttaaca ccgccacacc gcgactgttg gcagcttcaa ttaaatcgcc taacgcgtaa
     7441 gcacccgaca aatccaattc atccaaccat ttgcaacgaa tgatgagcac atctttgctg
     7501 tcaagatgtt ccaccgtctc gttcagccaa tcgatcgcac cgaaaaataa cggaccgtga
     7561 ggacggacga tgataatccg gtcccgaacg atatgctcaa aacgaccgct cagctctccc
     7621 atattggccc cattctggct gatttttctg gcataatccc tggcgaagga aaaaatgagg
     7681 tcttcttctt cctgtttcac atcttcatca cgttggctga gatccgcctt gtagcgatcg
     7741 gactggtcct tcacaaaacg gaaaaaggcc actgcgagtc ccatcgccat ggcggacagc
     7801 aggttccaaa acacggtcat caagaccacc agaccgaaca ccatcaaatc agacgtcggc
     7861 aatcttcgaa ccaccggcag gactcgcata tccagaatgt catagccggc tttgaggaga
     7921 ataccggcca aggcggaaag aggaatggat gcggccaatg aacccagtcc gaatatgaag
     7981 gaaagcagga caaaccccat ggtaatggac gacaaacggg tacgagcccc actctggata
     8041 ttcaccacac atggcatggt attcgtggct ccccccagcc ctccgaccaa tcctgaaaag
     8101 agattggctg atccttgggc caccaattca cggtcactat tgtgatgtgt gccggtcata
     8161 ttatcgacaa tgagacaagt caggaggcta tcaaatatac ataaccccgc cagagccgca
     8221 gccgggacaa aggcctttcc caagatatcg aggttgggaa gatatagctc aggtaagccc
     8281 gtgggaatat ctccaatttt tggaatgtct agcccgagga caaccgtggt taccgtaccg
     8341 accaacaatc cgatcaatgg acctggaagc caggtcacag gagaaatttt tggccaaacg
     8401 atgacaatga gtaaggtcaa caagccaatc cccaatgcca caggcgatgc atggaccaaa
     8461 ctggagggca attccgtaat ggcggaagta atacttttga cgccgggaag tcccaaaaat
     8521 gggttgagtt gcagcaacat gtacaacaca cccaaacccg tcataaatcc ggagatgacg
     8581 gaatagggtg tgaacttgat aaattgcccg agcttcaaca gccccaacgc aatcaaaata
     8641 aaaccagcta aggcgatggt cgtaaatacg aaaaccagat ctgggctccc atcggccaat
     8701 ttattggtct gagtcatgga aaggatttga atggtcatgg gtcccgtcgg cccaccaacc
     8761 gaccacgccg atccacttaa ggggcctgca ataagaccag ccgccactgc cgaccacatg
     8821 cccgcaatcg ccccgagccc ggaagccaca ccgaaggcca aggccatggg gagcaccacc
     8881 atggctaccg tgatccccgc cagcacatcc cctctcacat tactggcaat ccctttaaaa
     8941 ttttctttaa aattgaaatc cttaagatat atacgaatac ccgcagtcag gtgctccatg
     9001 cctaatttcc tccgactcat tcgtccaatt cgtcatcgcc tagacaagaa tgcaaaaata
     9061 tgcccttaca atgtacaata agcttcatgc gcaacttaca tgccaataat catcgaacag
     9121 atagggttca ttaaaatttc ttttattttt ctttataatt cctcaaaaat atgcgccgat
     9181 atcccatcac agttatagac tgatttttct cgctaagacc ttttgtgcga attcgtacgg
     9241 tgtgaactgt gcgaaagtac tcccgttacg acatgaacaa gaaaagagga cccttaccta
     9301 aaagggagga tgaaggaatg gacaagtgaa aatctatcag gactaattga ggaattagaa
     9361 tgtcaatgtc gccatggcat agacataatt gacatcggcc gtcccagggc tctgtggcac
     9421 tcgatcaaaa taggagcctt tgaaaaaacg cgtatttccc acctcaaatg ttgtatgtcg
     9481 gaaatacccg gcctgagggg cccacgttat ggagctatcc aacatattgc caaggaaagt
     9541 gcccgctcga ccggtcgggt cctgcagacc gctacccaca aaagggccgc ggccatctgc
     9601 cagccagaag gcgcgataag atgccaccaa cctaaccgtg gagatcggct ggagagtcga
     9661 gcgaaaccca ataggtgaaa aaatattgga tggaaagatg attcccaaaa ttccggttgg
     9721 tccatattct gcccggcgtt ttgcgaacaa gaaatcaaaa ttctttttcg ggttcctg
//
LOCUS       DATIAL010000024         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1509804, whole genome shotgun sequence.
ACCESSION   DATIAL010000024 DATIAL010000000
VERSION     DATIAL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509804"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VLA60_00825"
     CDS             <1..641
                     /locus_tag="VLA60_00825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HSF07923.1"
                     /translation="SYRRARMAIANGAFKQEIVSVEVPQKKGDPLLVTDDEEPNRVNL
                     EKLASLKPVFKEDGVLTVGNSPSCNDGAAALVLMEEQEAARHCIRPLAKIVGYAGAAL
                     APEWFTIAPVHAIQKVLKQTNLSIDQIDLFEINEAFSSVSLAINRELNLDPAKVNVNG
                     GAVALGHPIGATGSRIMTTLLYALEARGARLGLASLCIGGGEALAVVIERMT"
     gene            692..1555
                     /locus_tag="VLA60_00830"
     CDS             692..1555
                     /locus_tag="VLA60_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HSF07924.1"
                     /translation="MPSQAIQTIGVVGAGQMGAGIAFVSALAGCEVLLYDIHTGSLEA
                     ALERIHKGFDKQIQDGIIPASEGEAALKRIRTTQSLGDMTDRHLILEAAPENFEMKCE
                     LFRQLGAICKPEVVLASNTSAISITKLGAMSGRDAQVAGMHFMNPVPVMKLVELIRGL
                     RTSDETISLLAGLADRMGKVVVESKDSPGFIVNRLLMPMVNEAIFALAEGIATADMID
                     KAMTHGTNHPVGPLALADRIGLDTVLNICEILFHEFGDPKFRPCPLLRKYVEAGWLGR
                     KSGKGFYTYQP"
     gene            1579..>3227
                     /locus_tag="VLA60_00835"
     CDS             1579..>3227
                     /locus_tag="VLA60_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSF07925.1"
                     /translation="MKIAHDSPVVHQKGSAEERPASHHPQDRKTPCTSLSGLPIADRY
                     TQEDLKDWDPERDLGHPGDFPYTRGIHPAMYRSRLWTMRQFAGFGTAHDTNARFKYLL
                     DQGQTGLSVAFDMPTLMGLDSDDPQALGEVGYCGVAVSSLEDMATLFDGIPLDRVSVS
                     MTINGPAAVIFAMYLTLAERQGIPLNRLNGTLQNDILKEYIAQKEWLFPPAPHIRLIT
                     DTIGYCSEHVPKWHPISISGYHIREAGSTAVQELAFTLYDGLTYVDAAVQAGLPIDAF
                     APQLSFFFNSHNDFFEEIAKFRAARRLWAQEMKNRYNPNNPRSLQLRCHAQTAGCSLM
                     AQQPMNNVVRTTLQALAAVFGGTQSLHTNSMDETLSLPTQEAVTLALRTQQIIAHESG
                     VTNTVDPLAGSYFIEHLTNRMEEGAREYFRKLDAMGGMLAAIERGFPQREILEASQHY
                     QEEIDRKERIIVGVNEYVESEQYTIPTLRIGKEVEQYQRERLRSFRAKRDPKKVTEAL
                     HGIQWFAQSGENLLPHLMHAVKAKATLGEMCGALKGVFGTYR"
BASE COUNT          761 a          895 c          861 g          710 t
ORIGIN      
        1 aaagctaccg ccgggcccgc atggccatcg cgaacggcgc attcaaacag gaaatcgttt
       61 ccgtcgaagt gcctcaaaaa aaaggcgatc cccttctcgt caccgacgat gaggaaccga
      121 atcgcgtgaa tcttgaaaaa cttgccagct tgaagccggt gttcaaagaa gacggcgtgt
      181 tgacagtcgg caattctcca tcttgcaatg acggtgcggc agcccttgtc ctgatggaag
      241 aacaagaggc tgcgcgtcat tgcatcaggc ctcttgcaaa aattgtgggc tatgccggcg
      301 ccgcattagc accggagtgg tttaccatcg ccccggttca cgccatccaa aaagttctaa
      361 agcagacaaa cctgtcgatc gaccagatcg acctctttga aatcaatgaa gcctttagct
      421 cggtgtctct tgcaattaac cgggaactca acctggaccc ggcaaaagtc aatgtgaacg
      481 gaggcgccgt ggcgcttggc catccaatcg gagctaccgg ctcacggatt atgactaccc
      541 tgctttacgc actggaagcc agaggtgccc gtttggggct tgccagtctc tgtattggtg
      601 gaggcgaagc cctggctgtg gtgatcgaac gcatgaccta gctcatagta ttcgtaatca
      661 cgtttctgtt ttgactggag aagaagcgaa catgccatca caggccatac aaaccattgg
      721 tgtcgtagga gccggccaaa tgggtgcggg cattgcgttt gtatctgcgc tcgcaggctg
      781 tgaggtcctc ctctacgata tccacaccgg atccctggaa gcggcacttg aacgcattca
      841 caaggggttt gacaaacaaa ttcaggatgg catcatcccg gcttcggagg gcgaagcggc
      901 cttgaaacga atccggacca cccaatccct cggggacatg actgatcgtc acctgatcct
      961 ggaagccgcc cccgaaaact ttgaaatgaa atgcgagctg ttccgacagc ttggagccat
     1021 ctgcaagccg gaagtggtcc ttgccagtaa tacgtccgcg atatccatca caaaattagg
     1081 ggccatgtcg gggcgggacg ctcaggtagc cggcatgcat tttatgaatc cggtcccggt
     1141 catgaaattg gtcgaactca ttcgaggttt gcgaaccagc gatgagacca tctctcttct
     1201 cgccggactt gccgaccgca tgggaaaagt tgtggtggaa tccaaggatt ctccaggttt
     1261 cattgtcaat cgcctgctca tgccgatggt caatgaagcc atttttgcgc tcgccgaagg
     1321 cattgccact gcggacatga tcgacaaggc catgactcat ggcaccaacc accccgtagg
     1381 gccgctggcg ttagccgatc ggatcggatt ggataccgtg ctcaacattt gcgaaatttt
     1441 attccacgaa tttggggatc caaagtttcg cccgtgtccg cttcttcgaa aatatgttga
     1501 ggcgggttgg ttgggtcgca agtcaggaaa aggattctac acctatcaac cttgattccc
     1561 ttgtcaggat atgacgccat gaagattgct cacgattcac cggtggtcca tcagaagggt
     1621 tcggctgaag aacgtccggc ctctcaccat cctcaggaca ggaagacgcc gtgtacctcc
     1681 ttgtccggac tgccgattgc cgatcggtat acgcaggaag acctcaaaga ttgggatcct
     1741 gaacgtgacc tcgggcatcc tggagacttt ccttacacac gtggaattca tccggcgatg
     1801 taccgcagtc ggctatggac gatgcggcaa ttcgcgggat tcgggacggc tcatgacacc
     1861 aacgcccgtt tcaaatacct cctggatcag ggacaaaccg gtcttagcgt cgcgtttgat
     1921 atgccgaccc tcatggggct ggattccgat gatccccagg cccttggcga ggtaggctat
     1981 tgtggagttg cggtctcgtc actggaagat atggctacac tcttcgacgg aatccctttg
     2041 gatcgggtat cggtctccat gacgatcaat ggcccggccg cggtaatatt cgccatgtat
     2101 ctcactctgg ctgaacggca aggcatcccg ctgaatcgac tcaatggcac ccttcaaaac
     2161 gatattctca aagaatatat cgcacaaaaa gaatggttgt ttccgcctgc tccccacatc
     2221 cggttgatca cggacaccat cggctactgc agtgagcacg tgccgaaatg gcatcccatc
     2281 agcatcagcg ggtatcacat ccgggaagca ggatccaccg ccgtgcagga actggccttt
     2341 accctgtatg acgggctgac ctatgtcgat gccgccgttc aggcaggatt gccgatcgac
     2401 gcctttgccc cgcaactgtc ctttttcttt aattcgcata acgacttttt tgaggaaatt
     2461 gccaagtttc gtgctgcacg acgcttgtgg gcgcaggaaa tgaaaaaccg gtacaacccc
     2521 aacaatccac gctcgcttca gctccgctgc catgcccaga cagcaggctg ttccctcatg
     2581 gcccagcaac ccatgaacaa tgtggtgcgc acaacccttc aggctctggc agctgtgttc
     2641 gggggcacgc aatcgctcca taccaattcc atggatgaga cgctgtctct gcccactcag
     2701 gaggccgtga cgctggcgtt acggacccaa cagattattg ctcatgagag cggggtcacc
     2761 aacacggttg atcctctggc cggttcctac tttattgaac acctcaccaa ccggatggaa
     2821 gaaggtgccc gcgaatactt tcgcaagctg gatgccatgg gtggcatgtt ggccgccatc
     2881 gaacgggggt tcccgcaacg agaaattctg gaggcgtccc agcactacca ggaggaaatc
     2941 gaccggaaag aacgcatcat cgtcggcgtc aatgagtacg ttgagtcgga gcagtacacc
     3001 attcccaccc tgcgaatcgg gaaggaagtc gaacagtatc agcgggaacg attacgttcg
     3061 tttcgcgcca aacgggatcc caagaaagtg actgaagcgc ttcatggaat tcaatggttc
     3121 gcgcaatccg gcgaaaatct gttgccccac ctgatgcacg ccgtcaaagc caaggccaca
     3181 ttgggagaaa tgtgtggagc cttaaaggga gtctttggaa cttatcg
//
LOCUS       DATIAL010000025         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_219964, whole genome shotgun sequence.
ACCESSION   DATIAL010000025 DATIAL010000000
VERSION     DATIAL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219964"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2480)
                     /locus_tag="VLA60_00840"
     CDS             complement(<1..2480)
                     /locus_tag="VLA60_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSF07926.1"
                     /translation="MSESTTERQPNRLSHETSPYLLQHAYNPVDWFPWGEEALNLAKQ
                     ENKPILLSIGYSACHWCHVMERESFENQAIADLMNRYFVNIKVDREERPDLDEIYMQA
                     TTAMNQGHGGWPMTVFLTPNQEPIFAGTYFPPTDRYGRPGFGSILTNIGEGWKKDQSN
                     ITQQASRFTARLRNALQPASPLAVGAAEIEDAVKQYARDFDARYGGFGRAPKFPPATG
                     LSFLLRQYHWSQEKTILAMITKTLDGMAEGGLYDHIGGGFARYSTDDEWLAPHFEKML
                     YDNALLARTYVEAFQVTGENRYRQVATETLDYILREMTAQEGGFYSATDADSEGVEGK
                     FFVWTPEQIREILTNKQDATRFCAYYDITDEGNWEHTNIPRTKKSLETVAKELGCSPE
                     DLRETIMRTKSTVYQARLKRVPPGLDDKIITAWNGMMIGTMAEAGRVFNHQPYLDGAI
                     RAADFLLTTLSRPESRLWRTYRAGHAHLNACLEDYAYAAEAMIDVYEATGYERYLHEG
                     VSLAERLLEDFEDRDHGGFFTTAADHETLIIRGREGADGATPSGNAVAASALARLSFH
                     RDREDFRKAATNAIRAYGQQIGQVPRGFPKSLMVVDLLLRGPVELALVGSPTDKGYSQ
                     LRTAVNACFVPYRILAYRQELETETPHPLLTGKTLVDGQAALYVCKNFACQAPITDPQ
                     KAIDVLTYPQGTIPSPDPPVQALTSQGLSGHATPQGTGQYVARILASPQDSPPSSHGY
                     TSLGSTGLTTSRIGFGGYRINLGVEDHRRALEKSLQSGCNLIDTSTNYADGGSERLVG
                     VVLKDLIAKEVVSRDEVIVVSKIGYVQG"
BASE COUNT          584 a          662 c          716 g          675 t
ORIGIN      
        1 ttgccctgca catagccgat ttttgaaacc acgatgactt catcacgaga gacaacctct
       61 ttagcgatga gatccttgag cacgacaccc accaagcgtt cgctcccacc atcggcataa
      121 ttggtggacg tatcgataag gttgcacccg ctttgtaaag acttttcaag ggccctgcgg
      181 tggtcttcca ctccaaggtt gatccggtaa ccaccaaagc ctatgcgaga agtggtcaat
      241 ccggttgaac ctaaagacgt atatccatgg gaggaagggg gcgaatcctg agggctggcc
      301 aggattctgg ccacgtactg ccccgtccct tgaggagtgg catgtccgga aagcccctgg
      361 ctggtcagtg cttgcacggg tgggtctggt gaggggatgg ttccctgagg ataagtcaat
      421 acgtcaatgg ccttttgagg atcggtaatc ggagcctggc aggcaaagtt cttacagaca
      481 tagagcgcgg cttgcccatc gaccagggtt tttccagtta acaggggatg aggggtttca
      541 gtctccagct cttgccgata ggccaaaatg cgatacggta cgaaacacgc attgaccgcc
      601 gtgcggagtt ggctatatcc cttgtctgtg ggagatccca cgagggccaa ttccactggg
      661 cctcgtaata aaagatcgac gaccatgaga cttttgggaa accctcgtgg cacctgaccg
      721 atttgttgtc cataggcacg aatagcattg gtcgcggctt tccgaaagtc ttcgcgatca
      781 cggtgaaatg ataagcgggc taacgccgat gcggccaccg catttccact gggagttgca
      841 ccatcggcgc cttcccgccc ccggataatt aaggtctcat gatcggcggc tgtggtaaag
      901 aatcctccat ggtctcgatc ttcaaagtct tcaaggaggc gttctgctaa tgagacccct
      961 tcatggagat accgttcata ccccgtggct tcatagacat cgatcatcgc ctcggccgca
     1021 taggcataat cttccaagca ggcattgaga tgagcatgcc ctgcccgata ggtgcgccag
     1081 agccggcttt ccggccgtga aagcgtggtc agcaggaaat cggcagcccg gatggcacca
     1141 tccagatagg gttggtggtt gaatactcga ccggcttcgg ccatcgtgcc gatcatcatg
     1201 ccattccagg cagtgatgat tttgtcatcg agtcccggtg gtacacgttt caggcgagcc
     1261 tgatacacgg tggattttgt acgcatgatg gtctcacgta agtcttcagg ggaacagcct
     1321 aattccttcg ctacggtttc gagtgatttt tttgtgcgtg ggatgttggt gtgttcccaa
     1381 ttgccttcat ccgtgatgtc gtaatacgcg cagaagcgag tggcgtcttg tttgtttgtc
     1441 agaatttcac gtatctgttc cggcgtccac acgaaaaact tgccttctac tccttcgctg
     1501 tccgcatcag tggctgaata aaaaccacct tcctgtgcag tcatttctcg taaaatgtaa
     1561 tctaaggtct ctgtggcgac ctggcggtat cgattctcac ctgtgacttg aaacgcttca
     1621 acgtatgtcc gggcaaggag tgcgttgtca tacagcattt tttcaaagtg cggggctaac
     1681 cattcgtcat cggtggaata gcgagcaaat cctccaccga tatgatcata cagtccgcct
     1741 tcggccatcc catccagggt tttggtgatc atggcgagta ttgtcttctc ttgactccag
     1801 tgatattggc gcaggagaaa ggagaggccg gtggcaggtg gaaatttggg tgcacgtcca
     1861 aatcccccgt aacgtgcgtc gaaatcacgg gcatattgct tgacggcatc ttcaatctcg
     1921 gctgcaccaa cggcaagggg agatgcgggt tgcagggcgt ttcgcaggcg tgccgtgaat
     1981 cgactggctt gttgggtgat gttggattga tctttcttcc acccctcccc aatgtttgtc
     2041 aggattgatc cgaatcccgg acgaccatag cggtcggtgg gagggaaata ggttcccgca
     2101 aaaatgggtt cctgattagg ggtgagaaat acggtcatgg gccatccgcc gtgtccctgg
     2161 ttcatggcag tcgtggcctg catatagatt tcatccaaat ccgggcgttc ttccctgtcc
     2221 actttaatat tcacaaagta ccggttcatc agatccgcga tggcctgatt ttcaaaggat
     2281 tcccgctcca ttacgtggca ccaatggcag gctgaatagc ctatggagag caagataggc
     2341 ttattttctt gtttggccag attcagcgcc tcttctcccc aggggaacca gtctaccggg
     2401 ttgtaggcat gttggaggag ataagggctg gtttcgtgac tcagccgatt gggttggcgc
     2461 tctgttgtgg attcagacat aaaggctttt cactcaacgt gatgcaggat aataatagag
     2521 gcggtgtact ttgaaagaat tattacctta ggaaaaagaa gtatcatggt tgttgctctc
     2581 cgaggcttcg ttcatatagt aacaccttcc atccatcttc ctctgaaatg aaaccct
//
LOCUS       DATIAL010000026        15251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1187929, whole genome shotgun sequence.
ACCESSION   DATIAL010000026 DATIAL010000000
VERSION     DATIAL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15251
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187929"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VLA60_00845"
     CDS             complement(<1..1578)
                     /locus_tag="VLA60_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF07927.1"
                     /translation="MTDTLIEIERGGSTDKSRRTFKTMLLFPPEWVPTAPYLALPSLT
                     AVLRSYGHEVVQKDVNIEMYDWFFSDTFLIWVKLRMDQQRRGLNERKARNELTDLERD
                     RLECLASQDAIDVMELAERAIEAKAIVRGESFYDAGKLEWALQTFRDVMQFISAAYFP
                     ASLVFYPMESNLGYRSGVSTEVLACLDDDLVNVYRDVCRQLVLPAIQREQPEVIGVSV
                     GTQMQLLAGMTFCKMIKEEFPDIHVTVGGNVITRLKDDLPKRKQFFTQVFDSAILYEG
                     EHALVWLLEALAGERDWGKVPNLMWHQEGEVRVNDEIYTEKTTALPLPDFEGLPLDAY
                     FVPVRILPYLATRGCYWGRCTFCDHGQGYFDQYRGKPAHDVVREIKALKEKYQAEHFL
                     FSDESYPPALLKKVSQLLIEEQVGIKWTTLIRFEESLQDPEIWKQAVRAGCCTLYYGM
                     ESANERVLELMDKHAKKAVIENNLREAAKAGIWNHVMAFYGFPGETRDEAEETREFLL
                     ENKRYIHSVELFYFVAYR"
     gene            complement(1598..3493)
                     /locus_tag="VLA60_00850"
     CDS             complement(1598..3493)
                     /locus_tag="VLA60_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF07928.1"
                     /translation="MRINYDKGGLIRPSKIQLPRAPDFKESTNKAKILLVFPPDWYPS
                     EPYLSLPTLTAFLRSAGHDVVQKDVNLEMYDWYFSRDFLRRILKKVPQQLDRLKRIRK
                     SRELTDDEVDLQLALCNCTRGYISDLAERAEKAKEIVRSQEFYDSDKLEWVMNTFREV
                     TATISLVYAPARICMPPMETDLSYKLFMSSEVLEAVEDTQVNIYRDVFEEILKPAILA
                     EKPDVIGISIVLRQQLFSSMTFCALIKEQFPDIHVTIGGNTVTRLREVLPDIPNLFAL
                     FDSAIVYEGETALLRLVEAIASDGDLSHVPNLLFRDAEGIHLNSESYAENMGELPPPD
                     FDGLPLEKYFVPEPILPYLATRGCYWGRCEFCDHGEGYTAGYRTKRDWQIIEELTYLK
                     NKYQARNFHFTDESYPPALFRKLTKKLIESNLDIAWTTHIRFEKSLLEDQVWADARTS
                     GCKFLHMGYESGSERVLQLMDKATTTEVIQRSLELSSKHGVWNHVMGFFGFPGETYQE
                     AKFSIQFLEDNREHVHSIGFGTFDLSKHTPVAKNPEKFGITYYKNPEWDLALDYYFTV
                     KEGLSIEDAERVFEEFEQNHYAGWDLKIFVREYVFLYVAHFGTNKIPSLQFRLDSHDS
                     STKMASV"
     gene            3836..4348
                     /locus_tag="VLA60_00855"
     CDS             3836..4348
                     /locus_tag="VLA60_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07929.1"
                     /translation="MSNTIMPECRNWMSYLMDSLVNSGTMPSWEALQYLVTNLLGEAP
                     NPHLHQSKSPYETTQQLLHRIYGPIVEAASRTNELPAQAMIHMFTDISLMGKSAVLVV
                     YFPGHNAPHQLLLLDSVPAWDLVFENSSAFDNWAEERYQWIVKALQSISHPIESLMVE
                     SPDNLQPILV"
     gene            4633..4851
                     /locus_tag="VLA60_00860"
     CDS             4633..4851
                     /locus_tag="VLA60_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07930.1"
                     /translation="MPINVLLKNSPRGRGPEPSQALLKEIQALPEGEERTKEADLAAQ
                     EIQALWESQEIGISRWSGESGQRYSDPD"
     gene            complement(4964..8635)
                     /gene="metH"
                     /locus_tag="VLA60_00865"
     CDS             complement(4964..8635)
                     /gene="metH"
                     /locus_tag="VLA60_00865"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HSF07931.1"
                     /translation="MSTLEAALESRILVLDGAMGTMIQAHNLSEGDFRGTRFVDHACD
                     VKGNNDLLSLTQPKIIEDIHVQYLQVGADIIETNTFNSNAISLADYQMEDLAYDLNLA
                     SAQLAKRAVARVQAEKPGRTCWVAGALGPTNRTASMSPDVNNPAFRAVTFDDLAAAYY
                     DQVRGLVEGGVDLLLVETIFDTLNAKAAFYAIAQYSEEVHRQFPIMASVTITDLSGRT
                     LSGQLIEAFWNSISHANLLSVGINCALGAKQMRPYIEELSKVAPVYISCYPNAGLPNA
                     FGGFDETPERMGGDLRDFASQGWVNIVGGCCGSTPEHIRAIAGGVKDYAPHKKTHVPR
                     LTRLSGFEPLTIRPEGNFVNIGERTNVTGSPKFAKLILNGKLEEALAVARQQVEGGAQ
                     IIDVNMDEGLLDSQKAMVEFLNLIGSEPDIARVPIMIDSSKWSVIEAGLKCIQGKGVV
                     NSISLKEGEDQFLEQARKIRRFGAAVVVMAFDETGQADTLDRKVEICTRAYRLLTEKL
                     NFPPEDIIFDPNILTVATGMEEHNAYAVNYIEATRQIKATLPFCKVSGGVSNISFSFR
                     GNNTVREAIHSAFLYHAIKAGMDMGIVNAGQLGVYEEIPKDLLKLVEDVLLNRRPEAT
                     EELVTFAETVKQQGKATVIDDVWRQGTVEERLSHALVKGLVEFIDADVEEARQKYPKP
                     LDVIEGPLMDGMNVIGELFGAGKMFLPQVVKSARVMKKAVAYLLPFMDAEKVEGESRS
                     QGKILLATVKGDVHDIGKNIVGVVLACNNYEVLDLGVMVSCDKILQTARQEKVDLIGL
                     SGLITPSLDEMVHNAREMTREGFSIPLLIGGATTSKAHTAVKIAPGYTSPVVHVLDAS
                     LAVNVVRKLMSPDDKIAFVQDVQAKQQKTREAYLAKTTAKKFVSLEEARKRPFDILWE
                     TASIAKPSVLGCKVLEDVALETLVPLIDWSPFFHAWQLRGKFPKIFEDSVVGPKAKEL
                     FDDAQKLLQEIIDRKLLTAKGVYGLFPANSQGDDIIIYKDETRSERVSVFHTLRQQIE
                     KPQGDFYYALADFVAPQSSGKADYIGGFAVTAGLGIEALCQRFEQDHDDYNAIMAKSL
                     ADRLAEAFAEWLHREVRQEWGFGQDEQLTKEELIQEKYRGIRPAPGYPACPDHTEKQH
                     LFELLQVEKTVGIQLTESFAMYPAASVSGFYFAHPQAKYFSVGKVQQDQIQDYAKRKG
                     MDVSVVERWLSPNLSYDPT"
     gene            8859..9578
                     /locus_tag="VLA60_00870"
     CDS             8859..9578
                     /locus_tag="VLA60_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07932.1"
                     /translation="MWPTPGSAKPYFEDGYLGLTQEELRQTLGIPMAVRSRKAALRVF
                     SYYTFPDWEKYFKNLVSPGNGEDVYTYARNGIQVRYSFSYIPDLNEGKNIPTLYVQRC
                     EVEFSPAVPLQDIPSLVPEFVPPTEPSAPTFRSNLWVLLFKGPPSPEADFIVKEQGKE
                     KLTWSLAYQMFAINGIPNILSLDIPIDRLEITTQSLQLVRTQQRLTHEPILNPFSPEF
                     QKRLPAPSAPIKSVPQPHYED"
     gene            complement(9622..9975)
                     /locus_tag="VLA60_00875"
     CDS             complement(9622..9975)
                     /locus_tag="VLA60_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07933.1"
                     /translation="MFLEVEQNPNFEGSYFMRFKELGPPRRVSHVKSYDRISQGEWCE
                     VTGWCDDPDQPLCPAYAQRVEDSGAGSAFIVFGGNWGIRLKLANDANDWNIEDANQWG
                     EGYLSLGEERDLRFE"
     gene            10293..10478
                     /locus_tag="VLA60_00880"
     CDS             10293..10478
                     /locus_tag="VLA60_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07934.1"
                     /translation="MAGMSREQVMDAWGSPYQMNVSYTEKGIRREEWIYEDWLDSSTI
                     KHRYLYFEEGKLIGGWY"
     gene            complement(10581..10865)
                     /locus_tag="VLA60_00885"
     CDS             complement(10581..10865)
                     /locus_tag="VLA60_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07935.1"
                     /translation="MQPINLADPDAILEFLANVTLRGEGVTAENLMEYVMDEGFTEPT
                     YLSAKGEDPLAFYQGQPNAWGIYQIREWKRVLIISGGTGKERRAQITETP"
     gene            11005..11751
                     /locus_tag="VLA60_00890"
     CDS             11005..11751
                     /locus_tag="VLA60_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HSF07936.1"
                     /translation="MRKLSPEQFRDRIFDVLRRKHHWATPFLEGSTITKEKLNIYFHQ
                     EYVVYVRDFSVLLAQVLGKNPPWEARRLLATIIYEEETGGFSLGQPHQELFLHMMNGL
                     GFDRAGFRDVELLASSRGYREWLNQICQEEEWSVGAAVLSIFIKGTANDPEEVLYPKP
                     AQNQDEIEDIVRKHYLSQHQGLSPAFMDYIRAQHMFSAGSRKTVYDMVTHHTDESGDQ
                     IKVLSLLEEALNLWSRYQEGIARACGIRQA"
     gene            11930..12679
                     /locus_tag="VLA60_00895"
     CDS             11930..12679
                     /locus_tag="VLA60_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07937.1"
                     /translation="MQCSWTPEVETVLYEGPECVISLKTSHALKQAPEHPAFLSESLI
                     ANILNGMTISQEEGILQQLLLPKHHPVPAFSPSQIDFLAPHISMAFSQVTPEEVIHFT
                     CPPTNEQHFPIQGMLAVFPPSSLLLTLEKFKPYPGVSPKMQNTSQKLRPASLVFSHQE
                     SIIRAEEAQNLMAIPSTSHVISINYQSLDSLNPISKENQPTQPGVKATPRKHTEAPMT
                     IDSLDSQLRDLQKIVDQQAEEIRRLQDTSAQ"
     gene            13663..13860
                     /locus_tag="VLA60_00900"
     CDS             13663..13860
                     /locus_tag="VLA60_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07938.1"
                     /translation="MANGRQLTLVALITGESHPQEFAEQASQAGLQKALHFQHGPVKF
                     LERSQISQLSYFRTVRLRGKA"
     gene            complement(14147..14533)
                     /gene="mutT"
                     /locus_tag="VLA60_00905"
     CDS             complement(14147..14533)
                     /gene="mutT"
                     /locus_tag="VLA60_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HSF07939.1"
                     /translation="MAAAIIWRKDMILLARRMPESHLGGLWEFPGGKKEFGETLEDCL
                     RREVREELGVDISEPLPFHALHYQYPEKVVELNFYTCSIIQGIPRALECAEIAWVYKH
                     ELNSYAFPPADIPVVHKILQSGYLGE"
     gene            complement(14562..>15251)
                     /locus_tag="VLA60_00910"
     CDS             complement(14562..>15251)
                     /locus_tag="VLA60_00910"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HSF07940.1"
                     /translation="EKKSRLQAKVIRGIPKSKKTQFQKRLLTWYGEFGRDLPWRRTTD
                     PYKILVSEVMLQQTQVDRVIPKFHEFLGKYPTLQDLAVAHPDDIRRTWYPLGYNIRPY
                     RLHGIACETIERYGGAIPSKAEELLSLKGIGRYTAGAIRSFAFNKDAPILDTNVMRVL
                     HRIFIGKGEAKKQKTELWVLSETLIPRGKGYDFNQALMDFGAMVCTARKPMCLLCPMR
                     NICLTVSLDER"
BASE COUNT         4153 a         3964 c         3155 g         3979 t
ORIGIN      
        1 tcggtaggcc acaaaataaa acaattccac cgaatgaata taccgtttat tttccaggag
       61 aaattcccgt gtctcctcgg cttcatcccg tgtttcgccc ggaaaaccat agaaagccat
      121 cacatgattc caaattccgg ctttggcggc ttcgcgtaaa ttattttcaa tcaccgcctt
      181 tttcgcatgc ttatccataa gctctagtac acgctcattt gccgattcca tcccataata
      241 cagggtacaa cagccagctc gaacagcttg cttccatatt tcgggatcct gcaatgattc
      301 ttcaaaccgg attaaggtcg tccattttat ccccacctgc tcctcaatca acaattgcga
      361 aacctttttt aataacgcgg gaggatagga ttcatctgaa aacaaaaagt gctctgcctg
      421 gtatttctcc ttcaaggctt tgatttctcg gacaacatca tgcgcgggct ttccccggta
      481 ctgatcgaaa tatccctgtc catgatcaca aaaggtacat cgtccccagt aacaccctcg
      541 agtggccaag taaggtaaaa ttcgaaccgg gacaaaatag gcatccaacg gtaacccctc
      601 aaaatccgga agaggcaagg ccgtcgtctt ttcggtatat atttcatcat tcacacgaac
      661 ctccccttct tgatgccaca tcaagttagg gaccttgccc cagtctcgtt cacccgccag
      721 cgcttccagc aaccaaacca aggcatgttc gccttcatac aaaatggccg aatcaaagac
      781 ttgcgtgaaa aattgcttcc gttttgggag atcatctttt aaccgggtaa tcacgttgcc
      841 acccacggtc acatgaatgt caggaaattc ctctttaatc attttacaaa aggtcatacc
      901 cgccaggagt tgcatttggg ttccgacgga aactcctatc acttccggtt gttctctctg
      961 aatagcagga agcactaact gacggcacac atcccgatac acgttaacaa gatcgtcatc
     1021 caggcatgcc aatacttcag tcgaaactcc cgagcgatac cccagattac tttccatggg
     1081 atagaatacg agggaagcag ggaaataggc ggcagaaata aattgcataa catcgcgaaa
     1141 tgtttgtaag gcccattcca gttttcctgc atcataaaat gactcgcctc gcacaatggc
     1201 tttggcctct atcgctcgtt cagccaattc cattacatct atagcatctt gcgatgccaa
     1261 acactccagt cgatctcgtt ccaaatcggt caattcattg cgggctttcc gttcgttcaa
     1321 acctcgccgc tgctgatcca ttcgaagctt tacccagatt aaaaaggtat cactgaaaaa
     1381 ccagtcgtac atttcaatgt tgacatcttt ctgtaccacc tcatggccat aggagcggag
     1441 tacggcagtc agactgggta gagcaaggta tggagcggta ggaacccatt ccggaggaaa
     1501 gagcaacatc gttttaaaag tccgcctgga tttatcggtc gaaccaccac gctcgatttc
     1561 aattaaggta tcagtcatag ggtcaacgct cctgacgtta gacagaggcc attttcgttg
     1621 aggaatcgtg cgagtcgagc cgaaattgca gcgaaggtat tttattggtt ccgaagtggg
     1681 ccacgtataa aaacacatac tcccgcacga aaattttcaa atcccagcca gcataatgat
     1741 tttgctcaaa ttcctcgaat acccgttcgg catcctcaat actcaaccct tctttcaccg
     1801 taaaatagta atccaaggcc aaatcccatt ccgggttttt ataataggtg atgccaaatt
     1861 tttcaggatt ctttgcgacg ggggtatgtt tgctcaaatc aaaggtccca aatccgatcg
     1921 aatggacgtg ttcccgatta tcttctaaaa attgaataga gaatttggcc tcttggtaag
     1981 tttcccctgg gaatccaaaa aatcccatga catgattcca aaccccgtgt ttggaggaca
     2041 actccaagct tcgctggatc acctccgtcg tggtcgcctt atccatgagt tgaagcacac
     2101 gctcacttcc agattcatag cccatatgga ggaatttaca tcccgatgtc ctggcatccg
     2161 cccaaacttg gtcctcaaga agactctttt cgaaacggat atgtgtggtc caggcaatat
     2221 ccaaattcga ctcaatcaat ttcttggtga gcttccgaaa taaggcaggg ggatatgact
     2281 catctgtaaa atgaaaattt cgggcctgat acttattttt caaataggtg agttcttcaa
     2341 taatctgcca atcccttttg gtccgatacc cagcggtata gccctcacca tgatcacaga
     2401 actcacaccg cccccagtaa catccgcgag tggctaaata gggaagaatc ggctcaggta
     2461 caaagtattt ctctagtggc aatccgtcga aatctggagg aggtaactcg cccatatttt
     2521 cggcgtagga ttcggaattc aaatgaatcc cctctgcatc tctgaacagt aaattaggaa
     2581 catgtgataa atccccatcc gaggcgatgg cttccactaa tcgcaacagg gcagtttcac
     2641 cttcatagac aatcgcgcta tcaaataatg cgaagagatt tgggatatct ggaagcactt
     2701 ctctcaggcg ggtgacggta ttacctccga tggtcacatg aatatcgggg aattgctcct
     2761 taatgagtgc acaaaatgtc atagaggaaa acaattgctg tcgaagaaca attgaaatcc
     2821 caatgacatc aggcttttcg gccaaaatgg ctggcttcag aatttcctca aacacatccc
     2881 gatagatatt gacttgagta tcttcaacgg cctcaagcac ttcagacgac ataaagagtt
     2941 tataggataa atcggtttcc atcgggggca tgcagattct tgccggtgca tacaccaagg
     3001 atatcgtggc cgtcacttcc ctgaatgtgt tcatcaccca ttccagcttg tcactatcgt
     3061 aaaactcttg gcttcgaaca atttcctttg ctttctccgc tctttcagct aaatcgctga
     3121 tgtagcctcg ggtacaattg cagagagcta actgaaggtc cacttcatca tccgttaact
     3181 cacgactctt tctaatcctt ttgagacgat caagttgctg gggaactttt tttagaatgc
     3241 gacggaggaa gtcccggcta aaataccagt cgtacatttc tagattgaca tctttttgga
     3301 ctacgtcatg gccggcagat cgaagaaaag ccgttaaggt cggaaggctt aaatagggtt
     3361 ctgaggggta ccagtctggg ggaaagacca acaggatctt ggccttgttg gttgactctt
     3421 taaaatcagg cgctcttggt aattgaatct tagaggggcg aatcaaccca cctttatcat
     3481 aatttatcct catttttaca ccctaagtaa aattatttgt tcgcgtttta gtcaatcaaa
     3541 acgacatctt gaacaaccta tttcatcact atttcttatc ataaaataac tcattgattt
     3601 ttattagttt ttttcattag atagattcca cattctaagt ccttgaaaaa accatggtca
     3661 aggccaatcg cacggtcaaa caatattgaa agaaattaat atttgaccga ataatatctt
     3721 catattagaa tcactcatct gccctgggaa ttccttcctt gtctggtagc gtactcttca
     3781 tcaacaactc taaaatttct tagaatccct aagaaatggc tgtgaggaaa tgtccatgag
     3841 caatacaatc atgcctgaat gtcggaactg gatgtcgtac ttaatggatt cgctagtcaa
     3901 ctctgggact atgccctcat gggaagccct ccaatatcta gtgacgaatc tgctaggtga
     3961 ggcgcccaat ccccacctcc atcaaagcaa atcgccttat gaaacgacac aacaactcct
     4021 ccatcgtatt tatggaccaa tcgttgaagc cgcctcccgc acaaatgagc ttccagccca
     4081 agccatgatt catatgttta cggatatcag tctcatggga aaatcggcag tcttggtggt
     4141 gtattttcca ggacataacg ctccccatca attgttgcta ttggactcag ttcccgcctg
     4201 ggacttggta tttgaaaatt cttctgcatt cgataattgg gccgaagaac gatatcaatg
     4261 gatcgtgaaa gctcttcaga gcataagcca cccaattgaa tctttaatgg ttgaatcccc
     4321 cgacaacctc caaccgatcc tggtctaatc taagcatgct tatcaggaca tagacggtat
     4381 agcgaagttg ccagaatttt ccccgctatt gtgacaataa ggaagccttg gctaaaacgc
     4441 taccagcctt ggacaataat tttcatgggt ttcatagaaa gtacgctgtc ccaaggaaca
     4501 aagaaagtcc cacgtaaatg ggacattcac aaccggccat tctagccaga caagtccttt
     4561 cttttgtcag acttgacagt ccacgtgatg aggcgctatc catgaaacat tacgatacaa
     4621 catgaggtaa ctatgccaat caatgtgtta ctcaaaaact ccccgcgagg gcgcggtcca
     4681 gagccaagcc aggctctctt aaaagaaatt caggcattac cagaaggtga agagcggaca
     4741 aaagaagcgg atttggcagc tcaagaaatt caggccttat gggaatctca agaaattggg
     4801 atcagccgtt ggtcgggtga aagtggccag cgatattctg atccggatta gcactcatag
     4861 atttcagacc aactttctaa cactcctcct gaccctctcc caaaatttta ttagagggat
     4921 cccgccactc tcaaaaaatt gaacatcaaa attctctata tcctcaagta gggtcatagg
     4981 acaagtttgg ggaaagccag cgttccacca ccgagacatc catccctttc cgcttggcat
     5041 aatcttggat ttgatcctgc tgcacttttc caacggaaaa gtacttggct tggggatgcg
     5101 caaaatagaa tccactaact gacgcagccg gatacatggc aaaggattcg gtcagctgaa
     5161 tacctacggt tttctcaacc tgcaacaact cgaacaaatg ctgtttctca gtatggtccg
     5221 gacaggccgg ataccccggc gcgggccgaa ttccacgata cttttcttga atgagctcct
     5281 ctttggtcaa ttgttcatct tggccgaatc cccattcctg ccgcacttca cggtggagcc
     5341 attcggcaaa ggcctcagct aatcgatcgg ccaaagactt agccatgata gcattatagt
     5401 catcatgatc ctgttcaaac cgctgacaga gggcttcaat tccaaggccg gccgtgaccg
     5461 caaaaccacc gatatagtcc gcctttccgc tggactgcgg ggccacaaaa tcagccaatg
     5521 cataataaaa atccccttga ggcttttcta tttgctggcg caaagtatga aagactgaca
     5581 cacgttccga acgcgtctca tctttgtaga tgataatgtc atcgccttga ctattggctg
     5641 gaaacaatcc atagacacct ttggccgtaa gcaattttcg atcaatgatt tcttgcaaga
     5701 gcttttgagc gtcgtcaaag agttctttgg cctttggccc aaccacggag tcttcaaaaa
     5761 ttttcggaaa tttcccgcgc agttgccatg cgtggaaaaa gggggaccag tcaattaggg
     5821 gaacaagcgt ttcaagtgcc acatcctcaa gaactttgca accaagaaca ctaggcttgg
     5881 caatggacgc cgtctcccaa agaatgtcga agggccgctt tctcgcttct tccaatgata
     5941 caaatttttt ggccgttgtc ttggctagat aggcttcccg cgttttctgt tgtttcgcct
     6001 gtacatcctg cacaaaagcg attttatcat ccgggctcat taacttgcgc acgacattaa
     6061 ccgctaaaga cgcatctaat acatgcacga ctgggctggt atacccaggc gcaatcttca
     6121 ccgctgtatg ggccttactc gtggtggctc ccccaattag cagagggata gagaagcctt
     6181 ctcgagtcat ttctcgtgca ttatgaacca tttcatccag ggaaggagta atcagtccac
     6241 tcaggccaat cagatccact ttttcttggc gagcggtttg caatattttg tcacaggaaa
     6301 ccatgacacc cagatcaaga acttcatagt tattgcaggc cagcaccaca ccaacgatgt
     6361 tttttccaat gtcatgcacg tcgcccttca cggtcgcgag caggatttta ccctggcttc
     6421 gactctcacc ttcaactttt tccgcatcca taaacggcaa caaatacgcc acagcttttt
     6481 tcatgactcg cgcgcttttg accacctgag gcaaaaacat tttccccgcg ccaaacaact
     6541 ccccgatcac attcatccca tccattaaag gcccttcaat gacatccaga ggcttagggt
     6601 atttctgacg ggcttcctca acatcggcat caatgaattc gacaagtccc ttcaccaacg
     6661 catgcgagag acgttcttct accgtgccct ggcgccacac atcatcaatc acagttgctt
     6721 tgccctgttg tttgaccgtc tccgcgaagg taaccaattc ctcagtagct tccggccgac
     6781 gattcaacaa gacatcctct actaatttga ggagatcttt ggggatttct tcataaactc
     6841 ccaattggcc ggcattcaca attcccatgt ccatccccgc cttgatggca tggtagagaa
     6901 acgcggaatg tatggcttcg cgaaccgtat tatttccccg gaaggaaaag gaaatattgc
     6961 tgactccacc actgacttta cagaaaggta atgtggcttt gatttgcctc gtggcctcaa
     7021 tgtaattcac ggcataggca ttgtgttcct ccatgccagt tgcgacagtc aaaatgttgg
     7081 gatcaaaaat aatgtcctcc ggcggaaagt tcagcttctc agtcaggaga cgataggccc
     7141 tcgtacagat ctccactttt cggtcaagcg tatctgcttg acctgtttcg tcgaatgcca
     7201 taaccaccac ggcggcacca aagcgacgaa tcttccgcgc ctgctcaaga aattggtcct
     7261 cgccttcctt gagactaatg gaattgacca cacccttgcc ttgaatacac ttgagtcccg
     7321 cctcaatgac cgaccacttt gagctatcaa tcataatggg aacacgagca atatccggct
     7381 cggatccgat gagatttaaa aattctacca tggctttttg ggaatccagg agcccttcat
     7441 ccatattcac atcgatgatt tgcgcaccgc cctcgacttg ctggcgcgcc acagccaagg
     7501 cttcctcaag tttcccgttg agaatcaatt tggcaaattt cggtgaaccc gtcacattgg
     7561 tccgttcacc aatgttgaca aaattccctt ctggtcgaat agttaatggt tcaaatccgc
     7621 tcaaccgggt gagacgggga acatgggttt ttttgtgcgg agcataatcc ttgacccctc
     7681 ccgcaatcgc tcgaatatgc tcgggcgtac tcccgcagca cccacccaca atattgaccc
     7741 acccttggct ggcaaaatcg cgaagatctc cgcccattcg ctctggcgtc tcatcgaacc
     7801 cgccgaaggc attaggtaat cccgcattgg gataacaact gatatagaca ggcgctactt
     7861 tggatagctc ttcaatatag gggcgcattt gtttagcccc tagcgcgcaa ttaatcccca
     7921 cactcaaaag attggcatga gaaatggaat tccaaaacgc ttcgatcagt tgccccgata
     7981 acgtccggcc actgagatcc gtaatcgtga ctgaggccat aatagggaat tggcggtgca
     8041 cttcttcgct gtactgggca atagcataaa aagcggcctt cgcattcaac gtatcaaaaa
     8101 tggtttcgac cagaagaagg tcgacgccac cttccaccaa tccccgaacc tgatcataat
     8161 acgctgcggc taagtcatcg aaggtgaccg ctcgaaatgc cggattattt acatcaggcg
     8221 acatcgatgc agttcgattg gtggggccta aggccccggc gacccaacac gtccgaccag
     8281 gtttctctgc ctgtacccga gccaccgcac gcttggccaa ttgtgccgat gccagattta
     8341 aatcataggc caaatcctcc atctgataat cggctagaga aatggcattg gaattaaagg
     8401 tattcgtctc gatgatatcg gcgccaacct gaaggtattg cacatgaata tcttcaataa
     8461 tttttggttg cgtcaggctt agcagatcat tattgccttt gacatcacag gcatggtcca
     8521 cgaaacgcgt cccgcgaaaa tccccttcac tcagattatg ggcctgaatc atggtcccca
     8581 tggccccatc cagcacaagg attcgagatt ctaaagccgc ttctaatgtg ctcattctta
     8641 ccccctcatc attcaaaaaa catcccaact cacggtcaac ttatcaaggc ctatcagcca
     8701 tttctcatcc cccattcttg acttccagga atttcaagaa ataggattac gaaattcact
     8761 ctctttttat gacattttcc ttcccacttt gtaaaaggcc gtttttatgg gtggcctttg
     8821 ccgcattagt tacaatactt tgtagcatag ggggaatctt gtggcctacc cctggctcag
     8881 ccaaacccta tttcgaagac ggctatttag gtttaacaca agaagagcta cgccaaacac
     8941 taggaattcc gatggcggtt cgatcccgaa aagccgccct ccgtgttttc agctactaca
     9001 cgttccccga ttgggaaaaa tatttcaaaa atttggtttc accaggaaat ggcgaagatg
     9061 tttacaccta tgcccggaat ggcatccaag ttcgctattc cttctcctat attcccgatt
     9121 taaatgaagg caagaacatt ccgaccttgt atgttcagcg atgcgaagtg gaattttccc
     9181 ctgcggttcc cttacaagat attccttccc ttgtccctga atttgttcct ccgacagaac
     9241 cgtcagcgcc aaccttccga tccaatctct gggtcctgct gtttaagggg cctccttcac
     9301 cggaagccga tttcattgtc aaagaacaag gaaaagaaaa gctcacctgg tcgctggcct
     9361 accaaatgtt cgccattaat ggaattccca atatcctctc tctcgacatc ccgattgacc
     9421 ggctggaaat cacgactcaa agcctgcaac tcgtcaggac tcagcaacga ttaactcatg
     9481 aacccatcct caaccccttt tcacccgaat tccagaaacg tcttcctgct ccatcggcac
     9541 cgatcaaatc tgttcctcaa ccccactatg aggattgaac ttctgcccaa gttttgttta
     9601 ggatttaacc cttcgtcgac cctactcaaa tctgagatcc cgttcttctc ctaacgataa
     9661 atagccctca ccccattgat tggcgtcctc aatattccaa tcattggcat cgtttgcgag
     9721 ttttagtcga ataccccagt ttcctccaaa aacaataaag gctgaacctg cacctgaatc
     9781 ttctaccctt tgcgcataag caggacacag tggttgatca ggatcatcac accatcccgt
     9841 cacctcacac cattccccct gggaaatccg atcataggat tttacatggg acacccgccg
     9901 cggaggtccc aactccttaa acctcatgaa atatgaaccc tcaaaattcg ggttctgctc
     9961 aacttcaaga aacatacaat tctaccttga ctcgcctggg aatccactgt tagattaatg
    10021 agtaagtttc cttaattatg ataacacacg aaaatctaaa aatgccttca ccgtcattat
    10081 ctgcagagag aaacatttcc catccggaga ctgaacagct ctcccagccg acaagcaacg
    10141 aatctcaaat taccccatgg atctgtcgac gcccggttcg gctcacaata tatggttttt
    10201 tgatagcttt tgggctgtgg atggtcggat ggggcgttgt accacgatta cttgacccaa
    10261 cctttgagca tcacctccaa gataaatctg tcatggctgg gatgagtcgc gagcaagtga
    10321 tggatgcctg gggctcccct tatcaaatga atgtcagcta tacggaaaaa gggatacgac
    10381 gagaagaatg gatctatgaa gactggctgg attcaagcac gattaagcac agatatctct
    10441 actttgaaga agggaaatta ataggaggct ggtattgaga aatgttctgt ttcaacaaaa
    10501 gtaggagtga aacgctaatc atcagaaaaa gttagtccat tcctcactaa acctacacct
    10561 tgctcaaagt ttagatatgc ttaaggagtt tctgtaattt gggctcgtct ttcctttcca
    10621 gttcctccag aaataattaa aacccgtttc cattcgcgga tttgataaat cccccaggca
    10681 ttgggttgcc cctggtaaaa tgctagagga tcttcgcctt tggcacttaa gtacgtgggc
    10741 tcggtaaatc cctcatccat cacatattcc attaaatttt cggctgtgac cccttcgccc
    10801 cgtagagtca cgttcgctaa aaattctaaa atggcatctg gatcggccag gttaattggt
    10861 tgcattgtta ctgactcctt tgatatgtga taatgtattg tcaatcccta ggcacttatg
    10921 atgtctatca taatcccgta tttacttaaa agcccacctt ttcatttcaa gcaaatagac
    10981 cgttataagg gataacccct ctgtatgaga aaactttcac cagaacaatt tcgtgaccga
    11041 atatttgatg tgctgcgccg aaaacatcat tgggccactc cctttttgga aggtagcacc
    11101 attaccaaag aaaaattgaa tatttatttt catcaggaat acgtcgtcta tgttcgcgat
    11161 ttttccgtac tactcgctca ggttcttggg aaaaatcctc catgggaggc acgccggctg
    11221 cttgccacaa ttatctatga agaagaaacc ggaggtttct cccttggaca gcctcaccaa
    11281 gaactatttc ttcacatgat gaatggattg ggatttgata gggctggatt tcgggatgta
    11341 gaactcttgg catcaagccg tggatatcga gaatggttaa atcaaatatg ccaggaagaa
    11401 gagtggtcgg tgggagcggc agttctctca attttcataa aaggaacggc caacgatccg
    11461 gaagaagtgc tataccccaa gccagcgcaa aaccaggatg agattgaaga tatcgtccgg
    11521 aaacattatt taagtcagca ccaaggctta tcaccggcat ttatggatta tattcgtgct
    11581 caacatatgt tttccgcagg atctcgaaaa acggtgtatg acatggtgac gcatcatacc
    11641 gatgaaagcg gtgatcagat caaggtactc tcgctactag aagaggcatt gaacttgtgg
    11701 tcacgatatc aggagggaat tgcccgtgcc tgtggtattc gacaagcctg acaattacca
    11761 tccgcttttg ttctttattg ccgtcacgaa atttctggct tttgcttcct gaatagctgg
    11821 cgaagtgatc caagcactgc cacattcatt cacaccgttt ttaggtcatt tcaagccgct
    11881 aggattcact aaacaatgtt tgattgtcgc taccctggga atcttcaatt tgcagtgctc
    11941 ttggactcct gaagtagaaa cggtcctcta tgaagggcct gagtgcgtta tttccctaaa
    12001 aacatctcac gctctgaagc aggctccaga gcatccagca tttctttctg aatctcttat
    12061 tgccaatatt ttaaatggca tgaccatcag ccaagaggaa ggtatactcc aacaattact
    12121 gcttccaaag caccatcccg ttcccgcatt ctctccatct caaatcgatt ttttagcacc
    12181 ccacatatcc atggcatttt ctcaggtcac accagaggaa gttatccatt ttacgtgtcc
    12241 accgactaat gagcagcatt ttccgatcca gggaatgctt gccgtgtttc ctccatcctc
    12301 tcttctgttg actttggaaa aatttaaacc ctaccctggg gtttctccaa aaatgcaaaa
    12361 tacctctcaa aaactccgac cagcctctct ggtattttcc catcaggaat ctatcataag
    12421 ggcagaagag gcccaaaacc tcatggcaat tccctcgacg tcccatgtta tttccatcaa
    12481 ctatcaaagt ctcgattctt taaacccaat cagcaaagag aatcaaccaa cccaacctgg
    12541 cgtaaaggca acccctcgca aacatactga agcacccatg accatagatt cgctggactc
    12601 ccagttgcga gacctgcaaa aaatcgtaga ccaacaagct gaagaaattc gacgccttca
    12661 agacacctcg gctcaataga ataaatcata atcctacatc cagagattaa ctcccccgta
    12721 gaactgcatc tgggccatat aaatatcgaa acgtcatttg gtactggcaa acatggatta
    12781 caaatatacg tccttttccc ccacccgcat atcccaatta ctccaaatgg ccccatctcg
    12841 caaatgggtt cagtccgaaa ttttccagat tgatttcaaa cgacagttta actaaaactg
    12901 ccaaaaagtc ccttgctttg tatggggatg aagggcagcc cggagcgagg cggaggtacc
    12961 aaggcacaag tttctcggcc ttccccctgg ataagcccat gatgtcggtg cctttacgaa
    13021 aaaatactga agcccccggg gctgccaaga attgggtcag gggtttccgc ttaccttttc
    13081 ggtgaattgc gggctgggct atccctgagc accttataat cgacgcagtt tgggcaaggg
    13141 tcaatcgccc cggcgcaccg cattcgtccc cgtactcatg cccctccccg ctttggatat
    13201 ctgcggatag ggtccctttt cgaggtaaag ggtacctcgt aaatccggaa gggagttcgt
    13261 catctctctc gcctagaata atttcaactg cccatacggg taggaagacg aaaacccatc
    13321 gcgtggcatc gcggtgagac tcccacctct ataaggcaac tgaaaacagc acagttcatt
    13381 aggtcgggtc cacccagacg ggcacaaagg ttgtccccgc cttagaacgt gtgggaaaac
    13441 ggagtggata tcagaaaatg attggggtgg aaaatgggac tgaattcacg ccgaaggcat
    13501 tggatacctg ggcctagacg catggggtca cattcgattt cattcggcca gggaaaccct
    13561 cggagaatac ggtgattgag agtttcaacg gatgcgttcg agtcaaaatt tttatgccaa
    13621 tgggttcatg cccctacaag atgcccaaaa gaagattgga gagtggcgaa tggacgacaa
    13681 ctcacactgg tcgcattgat cactggggaa tctcaccccc aggagtttgc cgaacaggca
    13741 tctcaagccg ggctgcagaa ggcattgcat ttccaacatg gcccggtcaa gtttttggag
    13801 aggtctcaaa tcagccaatt atcctacttc cgaactgtcc gcttaagagg gaaagcttaa
    13861 aaagtgccca ctggggcctg gcatctgaga aaaccaagag accaattatc acaattcgac
    13921 cgaggactcg atcaaatgga tagcgtcaaa ccttatttcc cttatgggtg ctttctttgt
    13981 accccgaatt aggaaaaaca tcagggaagc gtgaggagcg ggcgtcggat agcagccaaa
    14041 tttcaatcct taacaatttt gctcgcttag gcatagtagc ctgaatggcc tagaaatcaa
    14101 ggacaatctc catgccccat gaagccttct ggaaaaccag gaaaactcat tcgcctaaat
    14161 accccgattg aaggattttg tgtacaacag gaatatctgc aggaggaaaa gcataggaat
    14221 tcaattcatg cttgtaaacc caggcgatct cagcgcactc taacgctcga ggaatgccct
    14281 ggatgatgga acaggtatag aaattcaact ccaccacctt ttctggatac tgataatgga
    14341 gagcatggaa cggaaggggc tcactaatat ccacacctaa ctcctctctt acctccctac
    14401 gcaagcaatc ttccagagtc tctccaaatt ccttctttcc tcctggaaat tcccaaagcc
    14461 cccctagatg ggattccggc attctccttg ccagaagaat catatctttc cgccagataa
    14521 tggcggcagc cacaacaata gtcgacgact cctgcagatc actacctttc atccaaagaa
    14581 acggtgaggc aaatattcct cattggacac aataaacaca tgggttttct ggccgtacac
    14641 accatggccc caaaatccat taacgcctga ttaaaatcat accctttgcc tctgggaatg
    14701 agtgtttcag agagaaccca taactcggtc ttctgtttct tggcctcccc tttcccaata
    14761 aatatccggt gcaatacccg catcacgtta gtgtctaaaa taggcgcatc tttattaaag
    14821 gcaaatgatc tgatggcccc tgcggtatac cgaccaatgc ccttcagaga aaggagctct
    14881 tccgccttgc tgggaatggc tccgccatag cgctcaatcg tctcacaggc aatgccatga
    14941 agacggtatg gtcgaatatt gtacccgagg ggataccacg tcctgcgaat atcatccggg
    15001 tgagctaccg ctagatcctg cagggtggga tatttcccaa gaaactcatg gaatttaggt
    15061 ataacccgat ccacctgtgt ctgttgcaac atgacctcag aaaccagaat cttatacgga
    15121 tcggtggttc tcctccatgg aagatcccgc ccgaattccc cgtaccatgt caagaggcgt
    15181 ttctggaatt gtgttttttt agattttgga atgccccgaa taaccttagc ttggagccta
    15241 gatttttttt c
//
LOCUS       DATIAL010000027        15750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1080611, whole genome shotgun sequence.
ACCESSION   DATIAL010000027 DATIAL010000000
VERSION     DATIAL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080611"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VLA60_00915"
     CDS             complement(<1..296)
                     /locus_tag="VLA60_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF07941.1"
                     /translation="MSEFTNQVILITGASSGIGKALACSLAPQAPQLVLVARDRARLQ
                     EVALACESLGASALIIPTDVTDPRACSAMIQQVVSVYSRIDVLVNNAGISMWSTV"
     gene            859..1455
                     /locus_tag="VLA60_00920"
     CDS             859..1455
                     /locus_tag="VLA60_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HSF07942.1"
                     /translation="MSIPSTRFQPYCLELPAWVADYLVQQPETYPTQDSRMDLVIKLA
                     QLNIQYGTGGPFGAGVFRLDTQELVAPGVNLVILSKSSIAHAEIVAMLMAQQIVGSHN
                     LGAIEFPTHELVTSCEPCSMCLGAISWSGVRQIVSGARGSDAEAVGFDEGPKPANWIL
                     ELEQRGIAVTTDIARAKAASVLQQYVRNGGEVYNGRKG"
     gene            1500..2333
                     /locus_tag="VLA60_00925"
     CDS             1500..2333
                     /locus_tag="VLA60_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HSF07943.1"
                     /translation="MTPAQNKSNASHSSTITLAFDIGGSGIKGLLLDQSGQPLSQRVR
                     MVTPKPATPKAIVPLISDIANTLGRFDRVSVGFPGVIHQGRVKTAPNLDASWPGTDLV
                     GELEQHLHKPVRAANDADVQGYGAIKGQGVEMVITLGTGFGTALFINGHLVPNLEIAH
                     HPFRKQQTYEVQLGASALKNVGKKRWNLRLAKAIDILDRVINFDHLYIGGGNSKKVTI
                     PLPPHATIVSNTAGLLGGIALWADPNVVIPSKPKVPGKGTARKQAPRKKSSKTLRKKK
                     N"
     gene            2429..2818
                     /gene="crcB"
                     /locus_tag="VLA60_00930"
     CDS             2429..2818
                     /gene="crcB"
                     /locus_tag="VLA60_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HSF07944.1"
                     /translation="MIPYGLPQLFWIGLGGFLGSVGRFVISGFFNRLSPALAFPIGTL
                     AVNILGCFLIGLLHGLAESRNMLGMDTRIFLFIGVLGGFTTYSTFGFESLALLKDGAM
                     LKASANIIIHVFVGLAAVWLGDTLGRL"
     gene            2967..5792
                     /gene="uvrA"
                     /locus_tag="VLA60_00935"
     CDS             2967..5792
                     /gene="uvrA"
                     /locus_tag="VLA60_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HSF07945.1"
                     /translation="MSSKKPDLPSSSPHADLIIEGARQNNLKNISLRIPHNAVTVITG
                     VSGSGKSSLAFDTLFAEGQWRYVESLSSYTRMFLDRVKRPDVDRLTNIRPSIALEQKN
                     PIRTSRSTVGTTSEISDYLRLLFSKIGRLTCPDCKVEAIAHHPTTIAQDLLNRFPNER
                     ALVCFPKPAPHPDALEFLNTSLLKQGFIRVVINHQLVNLNTDPLPSPLPSELSVVVDR
                     LTLDTESRSRLVEALESAFRESEGRAIVFIGDNQPLVFSAHLSCPGCGRTFPTPRPVS
                     FSFNHPLGACPECKGFGNILRYDERLLIPDPDKSLEDGAIEPWTKPSNVWWQKEMLKA
                     FKKKNLDPTTPYNQLTQVEQDLIWKGEGKLEGIDDFFSYLESKRYKMHVRVFLSRYRS
                     PFPCTTCQGTRLRPESLMVKIYDCHIHEVCGWPLSDLHRWLQTLPLRKFEEAIAKDLL
                     IALQTKLSFLLRVGLEYLTLNREMRTLSGGEAQRIHLATQLGSQLMGTQYVLDEPTIG
                     LHARDTEAMGMILRELAARGNTVIVVEHDPQIIQQADYIVELGPQSGNQGGEVICAAP
                     YQSFLKHPQSLTAKYLRGERTIPLPSTRRSGNGKTLQFNEVREQNLKNLTVNIPLGTL
                     VCVTGPSGSGKSTLVEATIYAALARFFKVDTPPQPELASMTGPEHLRTVCLIDQEPIG
                     KTPRSNPITYIKAYDEIRALFSQSPEARAHRLTPAHFSFNTGKGRCARCQGNGYEKLE
                     MYFLADLYVPCADCEGKRFKDKILQVQVKGLSIHDVLNLTVDQAVAFFATSCPKVLKG
                     LRVLQQLGLGYLTLGQPATTLSGGETQRLKIARELTNTPKRSAAHSDHKGALYILDEP
                     TRGLHLEDSTRLLTVLNQLVEEGNTVLVVEHHLDVIKCADWVIDLGPGGGESGGHIVA
                     QGPPKDIANSPTSITGKYLKTLFALGR"
     gene            complement(5914..6213)
                     /locus_tag="VLA60_00940"
     CDS             complement(5914..6213)
                     /locus_tag="VLA60_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSF07946.1"
                     /translation="MKIQILDEAQQDLIQGFRFYENREPGLGSYFLDSLFSDIDSLLL
                     YAGIHQVTYGYFRCLCKRFPFAIYYHVDEDLVRIHAVLDCRKDPLWIRSRLSKEN"
     gene            complement(6210..6428)
                     /locus_tag="VLA60_00945"
     CDS             complement(6210..6428)
                     /locus_tag="VLA60_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HSF07947.1"
                     /translation="MSINLPLKEMTLKEKLAVMELIWEDLVRTPDVVESPAWHRAVLE
                     ERGQRIAEGKSQFTDWEKAKLDIRNKLS"
     gene            complement(6692..7201)
                     /locus_tag="VLA60_00950"
     CDS             complement(6692..7201)
                     /locus_tag="VLA60_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07948.1"
                     /translation="MNEGSKDEHVKIVYAHFGLALYLAQVLEQGLANALMWAELLPRR
                     AEKPVSRKQWETEFDLFMNQQFEKTLGRLIRGLGKAASVSADLEGILTDALKTRNFLA
                     HHFFRERAESFMSRDGRNKMIEELERAQWLFDFAEVQLTEVAKPLREKYGFTDERLEP
                     FEQEYLKEV"
     gene            complement(8020..8871)
                     /locus_tag="VLA60_00955"
     CDS             complement(8020..8871)
                     /locus_tag="VLA60_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07949.1"
                     /translation="MKYYPVGDHIRVNGGCLDWLHEQGYPLEMRVAAAFRRTVPGASG
                     SDAAKIEWDHDVSQSWHYEDQDTGKSRELDVLFSHRDWLGMAEIHFCVECKSSSKPWV
                     LFTSNERESFNRILAFCVSSDDARSVLAKAITHLREAVPWFAKGGRVSYGITEAFTRP
                     DDATRSAILGATKASVSLVTRRSDGYSPPFLFAFPVVVISSRLFECYLATNGDLEVNE
                     VEEGWLHVRETFPGFSWTCVRVVTVSGLERFCKEVAETTFILRSALQGHIDQAWKKFK
                     IETQDPH"
     gene            9007..9534
                     /locus_tag="VLA60_00960"
     CDS             9007..9534
                     /locus_tag="VLA60_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07950.1"
                     /translation="MLFPSFQKPSLVRQEVLTLCFFVLLAVGIQGCSDSEDKPSPITQ
                     EKPAPTTSAIVPKDAQAPSSTSEQGNDSLPVTTPEPSRQPAQEELDHANFAIGKAEQL
                     LRRAPMGKESDLALSALERDLDSARTLLRTGQTHFDEQKFEMARTQAQQATEKASAVY
                     EHIEQAISTVNENSR"
     gene            9687..10358
                     /locus_tag="VLA60_00965"
     CDS             9687..10358
                     /locus_tag="VLA60_00965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSF07951.1"
                     /translation="MLPLLVGLASPFLNSVQSIPLSIAFLQTSQSLSQEGPPYDKPAQ
                     MPQTLFQEIESPSQTLQRASNLSPNQQPASTQAKISILVDVSRHRLFVKEGDRIVHEA
                     LASTGSGNTLADPRNPERTWTFETPKGTFSIESKLEKPVWIRPDWAFIEQGDPVPDKM
                     ADRLKPGVLGKYALGFGNGYFIHGALYSNLLGQDVTHGCIQLHSNDLQIVFKAVQLGT
                     PITII"
     gene            complement(10510..11064)
                     /locus_tag="VLA60_00970"
     CDS             complement(10510..11064)
                     /locus_tag="VLA60_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSF07952.1"
                     /translation="MKKSTISSILFLSLMFAGCSQYQVIPESLEHKVNHTLDFKQIRE
                     NPDNHKGELMVVGGEVLSVNRKQDATRIEVLQLPLNDDYTPANQRTKTQGRFIALSKG
                     KDPLDPAVLEKGRAISIVGEIIGSETIQVGEDTREVPIFGIKDLTIWDEALYWGRGYS
                     GFGWGRGYPADFYTGYRPLAYPYY"
     gene            11565..11987
                     /locus_tag="VLA60_00975"
     CDS             11565..11987
                     /locus_tag="VLA60_00975"
                     /inference="COORDINATES: protein motif:HMM:NF039411.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6335 family protein"
                     /protein_id="HSF07953.1"
                     /translation="MAKKKNSNSSRRDADTMIRRYIEDQDSAEEAIREEFIETGAQHE
                     SEVVLTRGNTRLRQARLVSEDFAATMDAGSSGDETVGGSNPTPDQDTVDELGEAVGLT
                     FENSEELAGEKVYERDTHRWELDPASSEDYTERIKKYR"
     gene            complement(12202..12564)
                     /locus_tag="VLA60_00980"
     CDS             complement(12202..12564)
                     /locus_tag="VLA60_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HSF07954.1"
                     /translation="MDHHHFSSSMEEELKLSDEQKEAFHRLRLDYDKMVVKKTADVRT
                     AEVDLAALLGKDEPDRQAIEEQVKTIGAIKEDMMMARIDSLLKLRGVLTKDQYGKFRE
                     ILHQRMSQVEGHSPHGGM"
     gene            complement(13070..13408)
                     /locus_tag="VLA60_00985"
     CDS             complement(13070..13408)
                     /locus_tag="VLA60_00985"
                     /inference="COORDINATES: protein motif:HMM:NF007493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HSF07955.1"
                     /translation="MQTISWDDFIKVELRVGRIVSASRFPEAKKPAYILQVDFGKDIG
                     MKKSSAQITDLYKPEDLVGMLVVAVVNFPNKQIGPFMSECLVTGFHNAGGEVALCVPD
                     KPVPLGTKLL"
     gene            13716..14078
                     /locus_tag="VLA60_00990"
     CDS             13716..14078
                     /locus_tag="VLA60_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07956.1"
                     /translation="MSKIWRLLLLLTCMLVGCSTTGTLGIVTKSSADPASLLKNSQGF
                     EELGPAEGQACRHFILAIIPFGQSDMAKAVDKALEATGGDALLNVSAESSLYGFIPIY
                     NVYTFTCTTVKGTAIKFK"
     gene            complement(15106..>15750)
                     /locus_tag="VLA60_00995"
     CDS             complement(15106..>15750)
                     /locus_tag="VLA60_00995"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF07957.1"
                     /translation="EGAGGFSFNQYLIVDEEPLLFHTGPRKMFPFVREAVASVLPVEK
                     LRYISFSHVEADECGSLNEWLAAAPQAVPLCGTVAAMVSINDLADRVPRALTDGERLT
                     LGKHSVRWFDTPHLPHAWECGLLMEERTSTLFCGDLFTQGGADHPPMTESDILGPSEA
                     FRHEMDYFSHTKNVRGILEKLASSSPTTLACMHGSAWRGDGVKLLRALGDALSA"
BASE COUNT         4050 a         4283 c         3586 g         3831 t
ORIGIN      
        1 accgtcgacc acatggatat cccggcatta ttcaccaaca catcaatccg ggaatacaca
       61 gaaaccacct gttggatcat cgcggaacag gcacggggat ccgtcacgtc cgtcggaatg
      121 atcagggcag acgctcccag gctttcacag gcgagcgcca cctcctgcaa acgtgcccga
      181 tctctcgcaa caagcaccag ctggggagcc tgtggcgcca acgaacaggc gagcgctttg
      241 ccaatacccg atgacgcccc ggtaatgaga atcacctggt tggtaaattc actcatcgtc
      301 ccttcctcag gaatcgaatg ggatggaccc tattgacaga tttttttaac gagcgtagca
      361 ttacatttgc gctcatgaaa aacgattatg aaaggaagga gagaatgagc gtacatctcc
      421 tttcaaaccg taaagaggaa agggggtctc aaatggacga ccttactcca ccggatcatg
      481 taggcccatc ggcggcgaaa cccgcctcag tctcctgacg ggccttctcg ttttcgccga
      541 atcatatccc tactcagaca caagcacgcc taagggcatc tgccagcggg cattgcaatg
      601 agcttgatga taatgagtca caagagggat cttcaacctt cacatctggt agattcggcc
      661 aaacccttaa cgagaaacgc gtcattagcc tactgatggg ccacgttaaa catgaggttc
      721 acattttgtg gtgtatgcca cataatccga ggggctccct ttcacgggac aagcccctcg
      781 ggttgttttt gatgccggca ccacaatcag gtaaccgttc ttcacacctt ttgccgcgca
      841 aactcctgac tccctgatat gagcatccct tctaccaggt ttcaaccata ttgccttgag
      901 ctaccagcct gggtcgccga ttatcttgtc caacaaccgg agacctaccc gactcaggat
      961 tcccgtatgg acctggtcat taagctggcg caactcaaca ttcaatatgg aacaggggga
     1021 ccatttggag caggggtttt ccgcttggac actcaagaac tggtcgcgcc aggggtgaat
     1081 ctcgtcattt tatcgaaaag ttcaattgcc catgctgaaa tcgtcgcgat gctgatggca
     1141 cagcaaatcg tgggctccca taatttagga gcaatcgaat ttcccaccca cgagctggta
     1201 accagttgcg agccctgcag tatgtgcctg ggagcgattt cctggtccgg cgttcggcaa
     1261 atcgtcagtg gcgccagagg aagcgacgcc gaagcggtgg gttttgacga aggccccaaa
     1321 cctgcaaatt ggattttgga acttgagcag agggggattg ccgtgaccac ggatattgcg
     1381 cgagccaagg ccgcgtctgt cctccagcaa tatgtccgaa acggcggaga ggtgtataac
     1441 ggccggaaag ggtagatgag ggccctattt cactttcaca attccaagga gaaacgttga
     1501 tgactcctgc acagaacaaa tcgaatgctt cacactcatc aaccataacc ctcgcctttg
     1561 atattggcgg gtccgggatc aaaggcctcc tgctcgatca gtccggccaa cctctgtctc
     1621 aacgggtacg aatggttaca cccaaacccg ctacgccaaa ggccatcgtc ccgcttattt
     1681 cggatattgc caacactctt ggccggtttg atcgggtatc agtgggcttt ccgggcgtca
     1741 ttcaccaggg gcgggtgaaa acagccccca acctggacgc atcctggcct ggaaccgatc
     1801 tcgttggaga actggaacaa cacctccata aaccggttcg ggcggcaaat gatgccgatg
     1861 tgcagggcta tggagcgatc aaaggccaag gggtcgaaat ggtcattacc ctgggaaccg
     1921 ggtttgggac tgcgttattt attaatgggc acctcgtacc aaatttggaa attgctcacc
     1981 acccctttcg gaaacaacaa acatacgagg tacaattggg tgccagcgca ctgaaaaatg
     2041 tggggaaaaa gagatggaat ctccgtctgg ccaaagcgat tgacatatta gatcgagtga
     2101 ttaatttcga ccatctctac ataggcgggg gcaacagcaa aaaagtcacc attccattgc
     2161 cacctcatgc cacaatcgtc tcaaatacag cggggctcct tgggggaatc gcgctctggg
     2221 ccgatcccaa tgtggtcatt ccatctaagc ctaaggtccc cggtaaagga accgcacgaa
     2281 aacaggctcc tcgaaaaaaa tcatcaaaaa ctctccggaa gaaaaagaat tgactcccat
     2341 aaacgcttcc ccaccactca gctcgttcca cgccaggttg cgccttccct tcatcctgag
     2401 ggaaatcacc taaccggcac acaccaccat gattccgtac ggattacctc aacttttctg
     2461 gattggtcta ggtgggtttt tagggtcggt aggccgattt gtgatctccg gcttctttaa
     2521 ccgcctgagt ccggctcttg ccttcccgat tggcactctg gccgtaaata ttctcggctg
     2581 tttcctgatc ggattgctcc atggattggc cgagtccagg aatatgctgg gaatggacac
     2641 cagaatcttt ctgtttatcg gcgttctggg tggattcacg acctactcca cattcgggtt
     2701 cgaatccctg gcactcctga aagacggtgc catgctcaaa gcctccgcca atatcatcat
     2761 ccatgttttc gtgggtctag ccgctgtctg gctgggcgat acattaggac ggctgtaagc
     2821 tcaagtccct tcttcctcct tatcaattca caaaatagat tttgggagtg gattccttct
     2881 cttgaagcca cttggcgaga agggggtgaa cacggtacaa ccattattcg tcgtgttcac
     2941 actccgaatg gtcggcatcc cagaatatgt cctcaaagaa accggattta ccttccagct
     3001 ccccccatgc cgatctgatc attgaaggcg cgcggcaaaa taaccttaaa aacatttctc
     3061 tccgtatccc acacaacgcc gtcaccgtca tcaccggtgt gtcaggatcc gggaaatcct
     3121 ctttggcctt cgacaccctg tttgcggaag gccagtggcg ttacgtggaa tcgctctcct
     3181 cctatacgcg catgttcctg gaccgtgtaa aacgccctga tgtggaccga ctcaccaata
     3241 tccgtccctc aattgccctg gaacaaaaaa atccgattcg gacctcgcgc tccaccgtcg
     3301 gcaccaccag tgaaatttct gattatctcc ggttgctatt ctctaaaatc ggacgactga
     3361 cctgtcccga ctgcaaggtg gaagccatcg cccaccaccc caccacaatt gcgcaagatt
     3421 tactgaatcg tttccccaat gaacgcgccc tcgtctgttt tcccaaacct gccccccatc
     3481 ctgacgcact ggagttcctg aacacgtcac tcttaaaaca aggcttcatt cgtgtcgtca
     3541 tcaaccacca actggtcaac ctcaacaccg accccctccc ctcaccgctg ccatcggaat
     3601 tgtcagtcgt cgttgatcgc ctgactctgg atacggaatc ccgcagccga ttggtcgaag
     3661 ccttggaatc agcatttcgc gaaagtgagg gccgcgcgat tgtcttcatc ggggataacc
     3721 aacccttagt gtttagcgcc catttgtcat gcccgggatg cggcaggacg ttccccacgc
     3781 cacgacccgt atccttttcc ttcaatcatc cacttggggc ctgtcccgaa tgcaaagggt
     3841 tcggcaacat cttgcgctac gatgaacggc tactgattcc cgaccccgac aaatccttag
     3901 aggacggggc gatcgaacca tggacaaaac cgtccaatgt gtggtggcag aaagaaatgc
     3961 tcaaagcctt caagaaaaaa aatctcgacc cgaccacgcc gtacaaccaa ctcacccaag
     4021 tagaacaaga cctgatttgg aaaggagaag gaaagctcga gggcattgat gatttttttt
     4081 cgtatctaga aagcaaacgc tacaagatgc atgtccgggt cttcctaagc cggtatcgca
     4141 gtcctttccc ctgcacaacc tgccagggaa cccgcttacg gccggaatct ctcatggtca
     4201 agatttatga ttgtcacatt cacgaggtat gcggatggcc cctctctgat cttcatcgat
     4261 ggcttcagac cttgccacta aggaaatttg aggaggcgat cgccaaggat ctgttgatag
     4321 ctttgcaaac gaaactgtca tttctcctgc gcgtgggttt ggagtatctc acactcaacc
     4381 gggaaatgcg gacgttatcc ggaggcgaag ctcagcgtat tcatctggcg acacaactgg
     4441 gcagtcagct catgggcaca cagtatgtcc tggatgaacc gacgatcggc ctccatgccc
     4501 gcgataccga ggccatgggc atgattttgc gagagttggc ggcgcggggc aataccgtca
     4561 ttgttgtgga acatgatccg cagatcattc aacaggccga ttacatcgtc gaactgggac
     4621 cacaatcagg caatcaaggc ggagaggtca tctgcgcggc cccctaccaa tcctttctca
     4681 agcatcccca atcgctcacc gcaaaatatt tgcgtgggga acgaaccata cccttacctt
     4741 ccacacgtcg atcgggcaac ggaaagaccc tgcaattcaa tgaagtacgc gaacagaatc
     4801 tcaaaaacct caccgtcaac attcccctcg gcacgctggt ctgtgtcacg ggcccttccg
     4861 gatcggggaa aagcacactg gtagaagcca ccatctatgc agctctggcg cggtttttta
     4921 aggtggacac tcctccccaa cccgagctgg caagcatgac ggggccggaa catctccgaa
     4981 ccgtctgcct tattgaccag gaacccatcg gcaaaacccc gcgctcgaat cccatcacct
     5041 acattaaagc ctatgatgaa atccgggccc tattttctca atcgccagaa gcgcgggcgc
     5101 accggctcac tcccgcgcat ttttccttta acaccggcaa gggccgatgc gctcgttgcc
     5161 agggaaacgg atacgaaaag ctggaaatgt attttctggc ggacctctac gtgccctgcg
     5221 cagactgtga aggcaaacgg tttaaagaca agatcctaca agtccaagtc aaagggcttt
     5281 ccattcatga tgtgttgaac ctcaccgtcg atcaagcggt agccttcttc gccacctcat
     5341 gtcccaaagt cctcaaagga ttgcgcgtgc ttcaacagct tggacttggc tatctcacac
     5401 tgggacaacc ggccacgact ctatccggag gcgaaaccca acggcttaaa atcgccaggg
     5461 aattgaccaa caccccaaaa cgatcagccg cccactcaga ccacaaaggg gccctctaca
     5521 tcctggacga acccaccagg ggactgcatc ttgaagattc cacgcgcctg ttaaccgtgt
     5581 tgaatcaact ggtggaggaa gggaatacgg tcctggtagt cgagcatcac ctggacgtca
     5641 tcaaatgcgc ggattgggtg attgacctgg ggccgggagg aggcgaatcc ggcggccaca
     5701 tcgtcgccca aggtccgccg aaagacatcg ccaatagtcc aacttccatt acgggaaaat
     5761 atttaaaaac tcttttcgct cttggacgat gaaaaatctt tttttctatc gtcttttgcc
     5821 atccctccac acagaaactt cctaaatgcc ccatgaatga tcgcttgtga agtagaaatg
     5881 gatattctac tcacaccatt ctgccgatgc cggttaattc tctttgctta atcggcttct
     5941 gatccataag ggatccttcc ggcaatctaa cacggcatgt atacgcacta aatcttcatc
     6001 gacgtgatag taaatggcaa agggaaaacg tttgcacaaa caccgaaaat aaccatacgt
     6061 gacctggtga attccggcat acaacagaag ggaatcaata tccgaaaaca ggctgtctaa
     6121 gaaataagac ccgagcccag gttcccgatt ttcataaaaa cgaaagccct ggattaaatc
     6181 ctgctgagcc tcatccagga tctggatttt cacgagagtt tgttacgaat gtcgagcttg
     6241 gctttctccc aatcggtaaa ttgagatttt ccctcggcga ttcgctgacc acgttcttcg
     6301 agaacagcac ggtgccaggc aggagattca accacatcgg gagtacgaac aaggtcctcc
     6361 caaatcaact ccatgaccgc aagcttttcc ttgagggtca tctctttcag aggtaggtta
     6421 attgacataa ggagagtata cctcagtgca taaggtaagg aaatccttga tatggcaaca
     6481 cacggatcat gaggtgatta atctagaccc cggcggcgcc attgtcgtcc aaggaacctc
     6541 ggaagcaatc gcccacaccc ctcagccact gaccggccaa tatctgaaac cacccttttc
     6601 ctaaactaac gctttgggtg agcggcgagc ggaggcgaat caaagcgccg acgtaaggag
     6661 cctgtctcca caggcttgtt agggatctat ttcaaacttc ttttagatat tcttgctcga
     6721 agggctctaa cctttcatcc gtgaagccat acttttctcg caagggcttg gcgacttcag
     6781 ttaactgcac ctcggcaaag tcgaacaacc actgcgctcg ctcgagctct tcaatcattt
     6841 tgttccgacc gtcacgggac atgaaggatt ctgctcgttc gcgaaagaag tggtgtgcga
     6901 ggaaattgcg cgttttcaag gcatcggtaa ggatgccttc caaatctgcc gaaaccgaag
     6961 cagcctttcc caaaccgcga atgagtctac caagcgtctt ttcgaattgt tggttcataa
     7021 ataggtcaaa ctcagtctcc cattgcttcc ttgaaactgg cttctcggct cgacgcggaa
     7081 gcagctcagc ccacataaga gcattcgcaa gtccttgttc caacacttgg gcaagataca
     7141 gagctaagcc aaaatgtgca tacacgattt tgacgtgctc atctttactg ccttcgttca
     7201 taatctttac taatacctag ctacggagcc gtttaagcac tgaatctccc attttctgtc
     7261 ggtaaacgac gctattaaat tgcgaatttt aagggtcatg tctctgaaaa tattagagac
     7321 ttacaaaatc ttaacctttt accatttctg gggggttggt cgtgcaatca aacgacaatc
     7381 ctcttacggc taaattcaac ggtatcatcc ggtgaaaatt ctttacttta ttacaaaagc
     7441 ttacccgaaa agtactcaaa ttcttattag atgtcgggtt tcagcccggc agccgaggtc
     7501 cttttgtttc ggcaaaaaga cccaaaacca ttgacaccca gttcggccac atgagattgg
     7561 gcagacgcga gaaggagagg ggcggaccaa ctcgctgcgc tcaaacaagg cccgccaatc
     7621 agggaggagc gtccgcccat ggggccgaac agcaggcgtc ggttcgaaag aaagagaacg
     7681 aaaaattttc tttggacagg tcttgcaatc acgcaattct ttttcggctt cactcaacaa
     7741 tatcaccaag aggcaatccg ctgtcgcatt gcaagactta cccctggacc ctttcatcca
     7801 agacatcaaa actttggcgc tgcaccagac gcattattca gccgaccttg catcgaaagg
     7861 caacactctc aacaaaagat ttactcactt tccacctctt accctccatc cagttgttcc
     7921 aatacgacac ggtcaaccct tcggccgcct gaaagtttgg gcaatcagca gcagcctcag
     7981 gctggcttct ggatcggcgg gtcatgtctc atctcgatcc tagtgcggat cctgagtttc
     8041 tatcttaaat ttcttccagg cttggtcgat gtgaccctgc agagcagagc gtaggataaa
     8101 agtggtctcc gcaacttcct tgcagaagcg ctcaagccct gaaacggtaa cgacacgcac
     8161 gcaggtccat gagaagccag ggaaggtctc tctgacgtga agccagccct cttcgacctc
     8221 attcacctca aggtcgccgt ttgttgcgag gtagcactca aacaagcgcg acgagatcac
     8281 aaccacaggg aaggcaaaga ggaaaggtgg agagtagcca tcggaccggc gagttacgag
     8341 tgagacgctt gccttcgttg cgccgaggat ggcgcttcgc gtcgcatcgt caggcctcgt
     8401 aaaggcttct gtgatcccat agctcacccg tcctcccttg gcaaaccaag ggactgcttc
     8461 ccggaggtga gtaatggcct tggcgagaac gcttcgggca tcatccgacg aaacacagaa
     8521 ggctagaatc cgattgaacg actcgcgctc attggaagtg aaaaggaccc acggtttgct
     8581 gctggatttg cattcgacac agaaatgaat ctccgccatg cccaaccagt cccggtgcga
     8641 aaacagcacg tcgagttcac gggatttgcc ggtgtcctga tcctcgtaat gccatgactg
     8701 cgacacatca tgatcccact cgatcttcgc cgcgtctgag cccgaggctc caggaaccgt
     8761 gcgtctaaag gccgcagcca ctctcatttc aagcggatag ccctgctcat gcagccagtc
     8821 taaacatccg ccattgactc gaatgtggtc tccaaccgga tagtatttca aaaggcagct
     8881 tcacgtcctt gtttcaatca tccccggttt tcttggataa aataggccag ggtttttacc
     8941 agctttctca aagccagtat ttcctttata gtagtagaac aaatcgtttt ttgaactcgt
     9001 actcttatgc tctttccttc ctttcaaaaa ccatccttag tgagacagga agtcctcact
     9061 ctctgcttct ttgttctctt ggccgtcggc attcaaggat gttctgattc cgaagataaa
     9121 ccctcaccga ttacccagga aaagccggca ccgacaacat cagcaattgt gccaaaagat
     9181 gcgcaggctc cctcaagcac gagcgagcaa ggaaatgatt ctctcccggt cacaacaccg
     9241 gaaccctccc gccaaccagc ccaggaagaa ttggatcacg cgaactttgc cataggaaaa
     9301 gctgaacaac tccttcgtcg agctcccatg ggcaaggaat cggacctggc attatccgct
     9361 ttggagcgag acctggattc ggctcgaacc cttcttcgaa ccggtcaaac ccattttgat
     9421 gaacagaaat tcgaaatggc ccgaactcag gcccaacaag ccacggaaaa agcttcggcc
     9481 gtgtatgagc acattgagca ggccatcagt acggttaacg aaaattcacg atagcttgaa
     9541 ggctttttgg ttaatttggt ctctggtggt gatgtccgat catggctccc gacaaccgag
     9601 atcttcaatg tccccgcaag acttttgaaa ctctcagaac caccggttcc ttagtttgct
     9661 gcgccgcggt agcttcggga gtcttgatgc ttcccttgct cgttggcctc gcttcacctt
     9721 tcctgaattc tgtccaatcc atcccgttat ccatagcctt cctccagacc tctcagagtc
     9781 tttcccaaga aggtcctccc tacgataagc cggcacaaat gccccagacc ctttttcaag
     9841 aaattgaatc cccctcccaa acacttcaac gcgcctcaaa tctctcaccg aatcaacaac
     9901 ccgcatccac gcaagcgaaa atatccatcc ttgtggatgt ttcccgacat cggcttttcg
     9961 taaaagaagg cgatcggatt gtgcatgagg cccttgcctc aaccggcagc gggaacacgt
    10021 tagcggatcc caggaatccg gaaagaacat ggacattcga aactcccaag gggacgttca
    10081 gcattgagag caagttagaa aaaccggtgt ggattcgtcc cgattgggcc ttcatcgaac
    10141 aaggcgatcc ggttcccgac aagatggctg atcggttaaa accgggagtg ctcgggaaat
    10201 atgcgttggg ttttggcaat ggatatttca ttcatggtgc cttgtattca aacttattgg
    10261 gacaggatgt cacccacgga tgcattcaac tgcattctaa cgatcttcaa attgtgttta
    10321 aagcagtcca attgggcacc ccaatcacca ttatttgaat aattccagtg ttcgtttctc
    10381 ttccgctcgt tggtttacat cccgggtttg atccaacaaa taaacctgct caagagggaa
    10441 tgaaaatggg aagatcttca ttaccctctc cttttattgt agtttgcagc cttactctcg
    10501 atgaaacaat cagtaatagg gatatgcgag cggacgatat ccggtataaa aatccgctgg
    10561 ataaccccgg ccccaaccaa atccggaata tccgcgaccc caatagagcg cctcgtccca
    10621 tatggtcaaa tctttgatgc caaagatggg aacctctcgt gtatcttcac caacttggat
    10681 agtttcacta cctataatct caccgacgat ggatatggct ctgccttttt caagcaccgc
    10741 tggatctaat ggatccttcc cctttgataa ggcaatgaac cgtccttgag ttttggtgcg
    10801 ttgatttgct ggcgtataat catcgttcag tggaagctga agaacttcaa ttctggtcgc
    10861 atcctgcttc cgattgaccg aaagcacttc tcctccgacg accattaact ctcctttatg
    10921 attatcaggg ttctctcgta tctgcttaaa gtccagggtg tggttgactt tatgttccaa
    10981 actctcgggg atcacctgat attgagaaca tccagcaaac atgagcgaaa gaaacagtat
    11041 ggatgagatg gttgactttt tcatggcagc ggtttctcct tagcgaattt aatacgagaa
    11101 aaaggtcgat aagaatgaaa gccaaacttt cgtgtgatta ttaataacaa ctgtatattt
    11161 cttaatgtta acgaaaccgc tcaggtttga atcctgccaa aaacccttgg ggaaagtgat
    11221 caaatacgga ccggaaaatt ttgtaaattg agtcatgggt ttcaaaaatt tcaggtattc
    11281 tccaaaattt catggtgcat acagtggatt ggatggtcac cgaataatcg gcccgatgaa
    11341 gaaatcgaac aaagacatat cgaaaatgaa ctcccagcca tgaccccttt ttaaacaatc
    11401 cccccacctc agcaaattcc tttcatcaat agaactctta cccgcctaaa catttcccag
    11461 ggttttctcc agtttgatac ttatgagcca tgggatacag tacagaccta acccgtttac
    11521 aaagttcata tatatttgct gcttatcaaa gatgaggagg atccatggcc aagaaaaaaa
    11581 attcgaattc atcacggcgg gatgcggata cgatgatacg gcgatatatt gaagatcagg
    11641 attctgctga agaggctatc agagaggaat ttatcgagac aggggctcag catgaatcgg
    11701 aggtagtcct tactcgtgga aacacccgac taagacaggc cagacttgtc agtgaagatt
    11761 ttgccgcaac catggacgcg gggagttccg gcgatgaaac cgtagggggt tccaatccta
    11821 ctccggatca agataccgtg gatgagttgg gcgaagcggt gggtcttacc tttgaaaatt
    11881 ctgaggaact tgcaggagaa aaagtgtatg aacgggatac ccaccgatgg gaactcgatc
    11941 ctgcatcctc tgaagattat acggaacgca tcaaaaaata ccgataagct tcttcagcac
    12001 agccagtaga agggttcaat gggaaagagc tttaaacagc ccgtactaca agcgggttcg
    12061 tatgggtggg tgagattgac tctcctcacc catccatatc atagccacaa gggaaaagcc
    12121 ttcgggtaaa acattcaagg cgtgtcagac tttcatccaa tttcacaccc ttcaaacact
    12181 cctaaactcc aaccgtgcgt gctacatacc gccatggggg gaatgtccct ctacctgact
    12241 catgcgttga tgaaggatct ccctaaattt tccgtattga tccttcgtca acacacctct
    12301 taatttcagc agggaatcaa tgcgcgccat catcatatct tccttaatgg ctccaatggt
    12361 tttgacctgt tcttcaatcg cctgacggtc tggctcatcc ttcccgagca gggccgcaag
    12421 atcgacttcc gccgttcgaa catcagcggt ttttttgacg accattttat cgtaatcaag
    12481 tcgaagccgg tgaaaggcct ctttttgttc atcgctcagc tttaattctt cttccattga
    12541 gctggaaaaa tgatgatgat ccatataatt ttgagttgca tgcggagaat cggattcgtg
    12601 cgtataggat tcatgagaac gttttccctc ggtagaactt tttccatcat gcgcacagcc
    12661 gcttaatgca aaaaccatag aaaggagaat aaggaaaccc tttctcccag gatcatgccg
    12721 aaatagattt tttaatgtag gtatgaacgc cctagtcgtc tcctatggtt aaaacggttg
    12781 gcattttttt cattgttgtt tgtgtccatt atcaaagagc agtaaacctc tcacacctgg
    12841 caagaaccct ggtgtcttgg tcagtaacct aaaatgagac aggccgggcc ggtgtatcca
    12901 acatactgaa aaagtccgct gtcagcctgt acttcatcat ccagccatct tcccacccat
    12961 cccacagaga cctcgtacga gttaaattgc tcgaagatta ggtggaactt tttttaaaac
    13021 ttccttcaag gcatggctct atcgggaacg tccccgagca acagagaaac tataacaact
    13081 ttgtgcccaa gggaacaggc ttgtccggta cacataacgc cacctccccg cccgcattat
    13141 gaaatcccgt gaccaggcat tcagacatga acggaccgat ttgcttgttt ggaaaattca
    13201 ctacggcgac aaccagcatt cccactaaat cttccggttt gtaaagatcc gtgatctggg
    13261 cgctcgactt cttcatgccg atgtccttcc cgaaatcaac ttgcagaatg taggcaggct
    13321 ttttggcttc cgggaaccgc gacgcactga caatgcgccc gacccggagt tcaactttta
    13381 tgaaatcatc ccaggatatg gtctgcacct tagcctccac aaattgctca caacagtttt
    13441 tcgtaataag aatagtaccc ttcccccatc cctttcagcc tctatctttg ccttacctcc
    13501 tagagttcaa ataaaatagt gattctccta atcctttcac cctggttggt cccgagctat
    13561 gatttcccgg ctagaggcta caccatttcc tcaaaaacac cgtgttccag gaccggttca
    13621 tttccccaag gaaagaccaa acgttttgct taagattgca agaggttatt ccgactgcca
    13681 agaatgagat ttaaccgtat cttaaggaga ataacatgtc caaaatctgg cgattgcttc
    13741 tgttactaac atgtatgttg gtgggatgca gtaccactgg aacgttggga attgtgacca
    13801 aaagctcagc cgatccagcg tcgcttttga aaaacagcca aggctttgag gagttaggac
    13861 cagctgaagg gcaagcttgc cgtcatttca ttttggccat tatcccattt ggccaatcgg
    13921 acatggctaa ggcagtggat aaggccttag aagccacggg cggagatgcc ttactcaatg
    13981 tctcagcaga aagcagcctg tacggattca ttcccattta caacgtctat acttttactt
    14041 gtactacggt caaaggaacc gccatcaaat ttaaatagag tactgaattg accttgattc
    14101 ttaggtaaac agaaattgga cggcttttat gtcggattgg tgcttgctat tccctcaatc
    14161 tcattcgatt gtcatactgt tgaagggcca ttgagtcatg agtatgcaca cctcggctta
    14221 gtccggctct gccgcttcgg cagccctgct tcccgcggtg taccgttaag ataaaattca
    14281 caatgttttg ccctggagca gggaagagaa gggtaaaccg catgcttcgt aagaattgct
    14341 ggttatattc attgaatgtt cgcactcttc tcaatattgt ggcgtgcaca taaaagttgg
    14401 atattctcag cttttaagga agtcccgccc tttgaatacg gaacaatatg atcgaaatga
    14461 agctcatcag tcgctccgca ggatacgcaa cggccgctat cccgtgccca tacctccaac
    14521 ttcacgcttg ttggaataat acgactacgc tctacaagtc tagtgggcat atcttcggct
    14581 aatgattcat cttcgatacc ttcaatcgcg actaacttga acttaaatac atttcttttt
    14641 ccatcgtgct cgatccacga atccacaagg tggaagtagc cgttatctga ccaaatcccc
    14701 gccttaattt tctcgtatac gcgaacaatg tcaggacctt tctcccccaa cttataagct
    14761 tgcgcagctt tgtgaaattt tccatcagag agttattttg ccttcgctct ggttggtcca
    14821 ggatttttgg gtcgacaccg ccgtttttct ttggctcatc atgcccttta tagaccagta
    14881 cagcgccatc ctccaacact acatccctat acggagcatt gggatgaacc gacatgagaa
    14941 ttactcaatg ctttcccttt aatcgaaaat tcatcccctt ctgtaaactg acgccttcct
    15001 tctggcacat ttcaatatag gcgatgatgt ttcccctcac cctggttcct tatgcatacc
    15061 tatgtattat tgtgagcctg agtgtcctgc tcggtatggc atgaatcatg ccgatagcgc
    15121 atcgcccagc gcgcgaagca atttgacgcc atcgccacgc caggcactgc cgtgcatgca
    15181 ggccagtgtg gtcgggcttg acgaggccag cttctcaaga atcccacgaa cgttttttgt
    15241 gtgtgaaaaa taatccattt cgtgccggaa cgcctcgctt ggcccaagaa tgtccgactc
    15301 cgtcatagga ggatggtcag cccctccctg cgtgaacaga tcgccgcaga acaacgtcga
    15361 cgtccgctct tccatcagta agccgcattc ccaggcgtga ggcagatgcg gcgtgtcgaa
    15421 ccaacgcacg gagtgtttgc ccagagtgag ccgctctcca tccgttaaag cgcgaggcac
    15481 ccgatcggca agatcattga tcgaaaccat ggccgctacc gtcccgcaca acggcacagc
    15541 ctggggagcg gcggccagcc attcgttgag tgacccgcat tcgtcggctt ccacatgaga
    15601 aaacgaaata tatcgcagct tctcaaccgg caacacgctt gccaccgctt cacgcacgaa
    15661 tgggaacatt tttcgcgggc cggtgtggaa gagcaatggc tcctcatcta cgatgaggta
    15721 ttggttaaac gaaaacccac ctgcaccttc
//
LOCUS       DATIAL010000028        14875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_113154, whole genome shotgun sequence.
ACCESSION   DATIAL010000028 DATIAL010000000
VERSION     DATIAL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14875
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113154"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..623)
                     /locus_tag="VLA60_01000"
     CDS             complement(183..623)
                     /locus_tag="VLA60_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07958.1"
                     /translation="MLWIVSAAVLGWFFIQGATIPSADGRTVVHLAPTERDFILTEMR
                     QLLQAVHGVVTGLSDPDQTQGHAQAAAAARSAGMKMAVDDNPSLMLKLPLPLKQLGMS
                     VHRDFDELADAITGGTTSQDILQRLSTITNRCLACHEMYQIKEG"
     gene            928..1500
                     /locus_tag="VLA60_01005"
     CDS             928..1500
                     /locus_tag="VLA60_01005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HSF07959.1"
                     /translation="MIQIRIHGRGGQGVVTAAELLALAAFRDGKEAQAFPSFGSERTG
                     APVTAFCRIDSQPIRSREPVYQPDVLLIQDSTLLHQVDVFAGLARSAFVLINSTRHLD
                     ALHLTDFVTQHPGVQMHTLPASDLALKQLGRPFANIGMVAGYAALTGEVSKASVTQAI
                     TEKFPGKIGELNGAVAAEVYDYVAANLAKV"
     gene            1527..2780
                     /gene="porA"
                     /locus_tag="VLA60_01010"
     CDS             1527..2780
                     /gene="porA"
                     /locus_tag="VLA60_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HSF07960.1"
                     /translation="MKHHIEGSQAVAHAVALCRPEVMACYPISPQTHIVETLSRKVKA
                     GEIGNCQFLNVESEFGALSVLIGASAMGARTYTATTSQGLLFMAEAVYNAAGLGLPIV
                     MTIANRAIGAPINIWNDHSDSMAMRDAGWIQLFAEDNQEAVDLHIQAFHLAEELSCPV
                     MVCMDGYILTHAYEVVDLPTQEQVDAYLPAFEPVQVLDPQDPVSIGAMVGPEAFAEVR
                     YLAHNKHLRALEEIPRLSQAFQDIFQRSSGGLLKAYHTDEAETILLALGSVNGTIKDA
                     VDDLRKEGWPVGSIALCAFRPFPSHALRHVVQDAKRIIVFEKDLAVGKGGIVSSDVKM
                     ALQGLPIIVDTVIAGLGGRPIPKASVMDTVKQACLEGLEEPHFLDLNWDAINRELTRQ
                     QAQRRSGPTPENILREIGAPGTSPI"
     gene            2801..3796
                     /locus_tag="VLA60_01015"
     CDS             2801..3796
                     /locus_tag="VLA60_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF07961.1"
                     /translation="MSYQRVKFYQTGTFTVGNRLLDESDRTVQAGMDRTNSLNSGHRA
                     CQGCGEALGARYALDAVMRATHQQMVAVNATGCLEVFSSPYPESAWQIPWMHSLFGNA
                     PAVASGVAAALRAKGKTNIRVIAQGGDGATVDIGFGCLSGMFERNDDVLYVCYDNEAY
                     MNTGVQRSGSTPPRAWTNTTQAVGTDPGNPMGIGKNLPRIAMAHGIPYVATASVADLH
                     DLEAKVTKAMSIRGARYIHIHVPCPLGWKSDPAHTIKVARLAVESGLFPLFEAEGGEV
                     TDRTPIRKKVPVEQYLELQGRFKHLLHPRDEGALKAIQGMADANIHRYQLLPLKA"
     gene            3796..5427
                     /locus_tag="VLA60_01020"
     CDS             3796..5427
                     /locus_tag="VLA60_01020"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HSF07962.1"
                     /translation="MMEQKPFAITLNPASSLANHTGTWRTMRPIYVDHLPPCNQGCPA
                     GENIQGWLYEAEEGKYEQAWRKIMEENPFPAIHGRVCYHPCETACNRGQLDEPVSIHA
                     VERFLGDYAIEQGWQVEAGVATGKRVLVVGAGPSGLSAAYHLTRFGHAVTIIEAGPKP
                     GGMMLFGIPQYRLPRNVVDAEIARIVAMGVSIHLNQKVDNLETTIQEGGFDAVFLAIG
                     AHLSKRVDIPGRDAGRMIDALGFLKGMDGQTVPKIGRRVVVYGGGDTAIDAARTAKRL
                     GAEETILIYRRDRGHMPAHDFEVEEALEEGVLTRWLHTIRQVDNTTLTVEEMTLDEQG
                     RPQPTGRIETLEADTVILAVGQEVDTGFLENVPGLDISSDGVIQVDALMMTGCAGVFA
                     GGDMVPAERTVTVATGHGKKAARHIDAYLRGSEYRKPSPNTVATYDRLNTWYYTDADK
                     SRQPYLDLARRRASFEEVVGGLDTDTALLEARRCLSCGNCFECDNCYGVCPDNAIIKL
                     GPGQRYEVNYDYCKGCGVCAEECPCGAIDMVKEVR"
     gene            5539..6405
                     /locus_tag="VLA60_01025"
     CDS             5539..6405
                     /locus_tag="VLA60_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF07963.1"
                     /translation="MKYLLAVDGSDQSVDAIRAFEALSPAESLMLLHVVNVPGIPYPS
                     IGAGIAKDLRLAVDKAMREEGERIIEQALSLLPLHPGSVMRRLEMGTPAEVILAMVKE
                     KGIDLVVLGARGIGQIREQMFGSVSHRVMTHASCSTLIIKKPTRKIQQVLIPVESQED
                     GEVVVRFLKKKPFREPITATVLHVIPFSEPVWPVGAMISPEFRKEMVAYGEKFTNGLS
                     AELKQMGHEAKGVVVVGAPSHTIIEEANKFAADVILMRTHSRSGVSRFLLGSVSHSVV
                     HHTESSILFVRE"
     gene            6960..7643
                     /locus_tag="VLA60_01030"
     CDS             6960..7643
                     /locus_tag="VLA60_01030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSF07964.1"
                     /translation="MINKSVRKIVSLLLGFALTFNLVCANNLWASPSVHQIPHLIIPI
                     LGTSLNKQFRPVGMVSQVVIDVYERQDRNGLQIQFHTEPGNFSLFARQSIHAAITRAL
                     AAANLPSESWTVLLKFPYNGLTVYGESLSAMVGLSVVSMIKGDHLLEGRALTGTITER
                     GSIGAVGGLQLKILAAYENHFERVLVPSEYDVRDGDWRTPFLMQVSPVGTVDEAYFGL
                     TGHHLATLP"
     gene            complement(7844..9169)
                     /locus_tag="VLA60_01035"
     CDS             complement(7844..9169)
                     /locus_tag="VLA60_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HSF07965.1"
                     /translation="MNDPLTNPDQSRTIKIDMMARVEGEGALHITVQDGQVRDVQLRI
                     FEPPRFFEAFLQGRHVNEVPDIVARICGICPVAYQMSAVHALEHIFDVEMPQSLRDLR
                     RLLYCGEWIESHVLHIYMLQGPDFLGFDSGLEMARSHPDLVKRGLRMKKAGNDLLTLL
                     GGRSVHPVSVKIGGFSQVPTRNALHGLRDEFLWARDAAAETLRWVSGFEFQDPEQAYT
                     FLALRGPSEYPMNEGDLVTNRGHRMAASEFEHHFHEEQVPYSNALHCTMNGQSYLTGP
                     LARLALNQDQLSLLAKDVLQSTGLVLPFNRASMGIVARSVEVLYAFDEALRLINQYER
                     PPAPDVPLTLRAGTGMAATEAPRGLLYHRYEVNADGTIRLAKIVPPTSQNQRRMEDDL
                     RNLLPGILHLNDQETAMKCEELIRSYDPCISCATHFLTLTKENLPPAMT"
     gene            complement(9367..10212)
                     /locus_tag="VLA60_01040"
     CDS             complement(9367..10212)
                     /locus_tag="VLA60_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07966.1"
                     /translation="MNTNGTTIPTQHPSTRPRPRIAVVKFASCDGCQLNILNMEDDLL
                     ALGEALDIAYFPEASSNMQPGPYDITFVEGSITCPEDLSRVLDLRQQTTTLITIGACA
                     TSGGIQALRNWGDVEAFKRTVYPSPEYIHSLSTSTPISEHVKVDYELWGCPIDKSQLL
                     RVLTDLLRGVTPSIPAHSVCLDCKRQGHVCILVAKGLACLGPVTRTGCGAICPAMGRE
                     CYGCFGPAEQDSVIPGVPPNTGSLAKQFHEHLQLIPLDMVRRFRGINGSVQPFRKESD
                     AWDSR"
     gene            complement(10471..11307)
                     /locus_tag="VLA60_01045"
     CDS             complement(10471..11307)
                     /locus_tag="VLA60_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HSF07967.1"
                     /translation="MSHNPYLIHPAVIVDKKQESTDIVTFRLQLNDPDHRKSFRFEAG
                     QFNMLYVFGVGEVAISIVSDPGEPEFLDHTIRVVGRITQVLGAMQVGESVGVRGPFGI
                     GWPMKEAQGKDVVIVTGGLGCAPVVGAIEYMFRRREEYGAMTILHGVKTPHDLLYRER
                     FDAWRHQPRTQVLLTSDEPGKTWHSHVGVVTELFDQLQLDPDNTLVMMCGPEIMMRIA
                     CNTLTHQGLRPDAMYVSLERHMECGIGLCGHCQLGPFFVCKDGPVMRLDRVVPFFGKV
                     GV"
     gene            11752..12189
                     /locus_tag="VLA60_01050"
     CDS             11752..12189
                     /locus_tag="VLA60_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSF07968.1"
                     /translation="MILADRLYTETDDTCALCGLRDTQALTIHHIDGNHGNDVYENTI
                     VLCHNCHIRYHQMKGITEEDIRTRKRHLMYKTITTYGVNALKIAARNNFGVIAMPFLL
                     YHLVGLGYMDKKEDQMGYGAQSDATARFAITDRGRNTLREWFE"
     gene            12889..13191
                     /locus_tag="VLA60_01055"
     CDS             12889..13191
                     /locus_tag="VLA60_01055"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF07969.1"
                     /translation="MQYVTPKKFAVPLNRQEVSQSWNQRGYSCDVFIDPPGREWNDFV
                     HSTNELVIVVEGKLRMTIEGEEIIVEPGDEVFIPREACHSVKNIHHATTKWLYGYD"
     gene            13424..13900
                     /locus_tag="VLA60_01060"
     CDS             13424..13900
                     /locus_tag="VLA60_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07970.1"
                     /translation="MMRFIRLSDYTWWIWLCLAGCLLAGLLVDPTYFFLAILISTIQA
                     LVFFAREENALSFPAQLRLAYLLLLVVLYLPPLRFLYWVPAIGTLILCFTGYCLLARG
                     LSLLPWNRDSPLTFQEVIRTFTVPPNLDVTLRGKPASGCPGGVCSLEVQIGKSAKA"
     gene            14213..14500
                     /locus_tag="VLA60_01065"
     CDS             14213..14500
                     /locus_tag="VLA60_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSF07971.1"
                     /translation="MASVGYHEPIEELSDETRDMHRAIVSLMEELEAVDWYNQRTNAC
                     KDPDLKAILAHNRDEEKEHASMVLEWIRRKDPTFSKHLKDYLFTEKPIAHK"
BASE COUNT         3514 a         3598 c         4074 g         3689 t
ORIGIN      
        1 cctcgcctgc cggggcgaaa cccggcaaca ccgaacatca tgtggacacg aaagtttgag
       61 gacacgagtg cgatccgttc ttctcacaac gtttttttgg caaccccaca ggatagattc
      121 ccgctacaca ttgtcaggcc ataaggtcag aactaaaaaa tctgtatgct cgcttttacc
      181 ctctacccct ctttaatttg atacatctcg tggcatgcca ggcatctgtt cgtgatagtc
      241 gacaggcgtt gaagaatgtc ctgtgatgtc gtgcccccgg tgatcgcatc cgccaattcg
      301 tcaaaatcac gatgtacgga cattccaagc tgtttgaggg gaagaggaag tttaagcatg
      361 agcgagggat tgtcatccac ggccatcttc attccggccg aacgggcagc cgctgcagcc
      421 tgagcatgtc cttgggtctg gtcaggatcg ctcaggcccg tcacgacgcc atgcacggcc
      481 tgaaggagtt gccgcatttc cgtcaggata aagtcacgtt cggttggagc aagatgcaca
      541 acagttcggc catcggctga agggatggtc gccccttgaa tgaaaaacca gcccagcacg
      601 gcggcgctga ctatccagag catgagagta atccggcaaa gattttttga gaatgaaggt
      661 aaagatgcca actggagtct ccttattttt caataacctg gtcgtttagg gaatcaactg
      721 gggcattatt ctacaggact gatgattttg actgtacctc ttttccccaa tgctccgact
      781 catcgttttt ggcctattgg agattgttga ggttttgcta gttatatcta ccatttcaag
      841 ttctcacaaa ataaggcata tctcttgcca aatacattat cagtgagtgc tgaggacagc
      901 gatttcaggt gaattgagag gaggcccatg atccaaatcc gtattcatgg cagaggaggg
      961 cagggcgtgg tgactgcggc ggagttgctt gcgttagcgg cattccggga tggaaaggag
     1021 gcgcaggcct tccccagttt tggatctgaa cggacgggtg cgccggtgac cgcattctgc
     1081 cggatcgatt ctcaacccat tcgcagtcgg gaaccggtct atcagccgga tgtcttgctc
     1141 atacaggact ccaccctgct gcatcaggtg gatgtttttg ccggattggc taggtcagcc
     1201 tttgttctga ttaattccac acgacatctg gacgcgcttc atctcacgga ttttgtcacc
     1261 caacatcccg gagtacaaat gcatacactc ccggcatccg atctggccct gaaacaactt
     1321 gggcgaccgt ttgccaatat cgggatggtg gccggttatg cggccctgac cggggaagtc
     1381 agcaaagctt ccgtgactca ggccatcaca gagaagtttc ctggaaagat tggagaattg
     1441 aacggggcgg tggccgcaga ggtgtatgat tatgttgcgg caaatctggc caaggtctaa
     1501 ttcaatcgag gaatcaacag aaggccatga aacaccatat tgaaggatct caagccgtgg
     1561 cccatgccgt cgctttgtgt cggcctgaag tgatggcttg ttatccgatc tctcctcaaa
     1621 cccatattgt tgaaaccctt tcacgcaagg tgaaagcggg agaaatcggc aattgccaat
     1681 tcctgaatgt cgaatcggag ttcggggcct taagcgtgct gatcggcgcc tcggccatgg
     1741 gggcacgaac ctatactgcc acgacaagcc aggggctctt gttcatggcc gaggccgtct
     1801 ataacgcggc gggattgggt cttcccattg tcatgacgat tgccaatcgc gcaatcggcg
     1861 cgccgatcaa tatctggaac gatcattcgg atagcatggc catgcgggat gccggctgga
     1921 ttcaattgtt tgcggaagat aatcaagaag ccgtcgatct tcacattcaa gcttttcacc
     1981 tggctgagga actcagttgc ccggtaatgg tctgtatgga tggctatatt ctcactcatg
     2041 cctatgaggt ggtggatctt cccacgcaag agcaggtgga tgcgtacctg ccggcgtttg
     2101 aacccgttca agtgttagat ccacaggatc ctgtgtccat tggcgcaatg gtcggaccgg
     2161 aagcctttgc cgaggtgcgg tatctcgcgc ataacaagca tctgcgagcg ttagaggaga
     2221 tcccccgact ctcccaggct tttcaggaca tctttcagcg gagttccggt ggactgttga
     2281 aggcctatca cacggatgag gctgaaacca ttctgctggc actgggatcg gtgaacggta
     2341 caattaaaga cgcggtggat gatttgcgga aagaaggatg gccggtcggt tctattgcgc
     2401 tctgtgcatt tcgacccttc ccctcccacg ctcttcgtca tgttgttcag gatgccaagc
     2461 ggatcattgt ttttgaaaaa gacctggcgg tgggaaaggg ggggattgtg tccagcgacg
     2521 tgaagatggc gctccagggg ttaccgatta tcgtggatac ggtgatcgcc ggacttggag
     2581 gtcgcccgat tcctaaagcc tcggtcatgg atacggtgaa gcaagcatgt ctggagggtt
     2641 tggaggaacc tcatttcctc gatctgaatt gggacgccat taatcgggaa ttgaccagac
     2701 aacaggcgca gcggcgttcg ggacccaccc cggaaaatat tctccgggaa atcggtgctc
     2761 ccggcacttc gcccatttaa tctttactga tgaggccgct atgtcctatc aacgggtgaa
     2821 attttatcaa accgggacat tcacggttgg caaccggttg ctggatgaat ccgatcgcac
     2881 ggtccaagcc gggatggatc gaacgaattc actcaattcc ggccatcgtg cctgccaggg
     2941 gtgtggcgaa gcgctgggcg cccggtatgc ccttgatgcg gtgatgcggg ccacccatca
     3001 gcagatggtt gcggtgaatg ccaccggctg tttggaagtc ttctccagtc cctatccgga
     3061 aagtgcctgg cagattccct ggatgcattc gttgttcggg aatgcaccgg cggtggcgag
     3121 cggcgtggcg gcagccctgc gcgccaaagg caagacgaat atccgagtca tcgcccaggg
     3181 cggagatggt gcgacggttg atatcgggtt cgggtgtttg tccgggatgt tcgaacgcaa
     3241 tgatgacgtg ttgtatgtct gttatgacaa cgaggcctac atgaacacgg gagtgcagcg
     3301 ttcgggatcc accccgccac gagcctggac caataccacg caggccgtgg gaaccgaccc
     3361 cggcaatccc atgggcattg ggaaaaatct gccacgcatt gcgatggccc atggcattcc
     3421 ctatgtggcc acggcctccg tggccgatct ccatgatctg gaagcgaaag tgacgaaggc
     3481 catgagcatc aggggtgcac ggtatattca tatccatgtg ccctgtccgc ttggctggaa
     3541 atcggatcct gctcatacga tcaaggtcgc ccgcctggca gtggaaagcg gcctatttcc
     3601 tttgtttgaa gccgaagggg gcgaagtgac cgatcgaacg cctatccgga aaaaagttcc
     3661 tgtcgagcaa tacctcgaac ttcaagggcg gttcaaacat ctcttgcatc cacgagatga
     3721 gggtgctctt aaagcgattc aggggatggc ggatgcgaat attcatcggt atcaactcct
     3781 tccgttaaag gcgtgatgat ggaacagaaa ccctttgcca ttacattgaa ccccgcttca
     3841 agcctggcca atcataccgg cacctggcga acgatgcggc cgatctatgt tgatcacctt
     3901 ccgccctgca atcagggctg cccggctggt gaaaatatcc aaggctggtt gtatgaggcg
     3961 gaggaaggga aatatgaaca ggcctggcga aaaattatgg aagagaatcc cttcccggcg
     4021 attcatggtc gcgtgtgtta tcacccgtgt gaaacggcct gcaaccgggg gcaactcgat
     4081 gagcccgtaa gcattcatgc cgttgagcga tttttaggcg attacgcgat tgagcagggt
     4141 tggcaggtgg aggcaggggt ggccaccgga aagcgggtcc tggtagtcgg ggcggggccg
     4201 agcggtcttt ctgctgcgta tcatctcact cgttttggac atgcggtgac aatcattgag
     4261 gcggggccga agccgggagg aatgatgctg ttcgggattc cacaataccg tctgccccga
     4321 aatgtcgtgg acgctgaaat tgcccgaatt gtggccatgg gcgtttcgat ccatctgaat
     4381 caaaaagtcg ataatttaga aacgaccatc caagagggcg gattcgatgc cgtgtttctg
     4441 gccattgggg ctcatctgag taaacgggtt gatattcccg gccgggatgc cggccggatg
     4501 atcgatgcct tgggatttct taaaggaatg gatggccaga cggttcccaa gatcggtcgg
     4561 cgggtcgtcg tctatggtgg aggtgatacg gctattgatg ccgcgcgaac cgccaagcgc
     4621 ctgggtgccg aggaaacgat cctgatttat cggcgtgatc gcgggcacat gcccgcccat
     4681 gattttgaag tggaagaagc cttggaggag ggggtgctga cccgatggct gcataccatc
     4741 agacaagtgg acaatacgac gttgaccgtt gaagagatga cgctggatga gcaggggcgc
     4801 cctcaaccca ccggccggat tgagaccttg gaagccgaca cggttattct ggcggtaggc
     4861 caggaggtgg ataccgggtt tttagagaat gtgccgggat tggatatttc gtccgatggg
     4921 gtgattcaag tcgatgcctt aatgatgacc gggtgtgcgg gagtgtttgc cgggggggat
     4981 atggttcctg cagagcgcac ggtgacggtg gccaccggcc atgggaaaaa agcggcacgg
     5041 catattgatg cgtatctccg cggaagtgag tatcggaaac catcgcccaa taccgttgcg
     5101 acttatgacc ggctcaatac ctggtattac accgatgcgg ataagagtcg gcagccctac
     5161 ctggatctgg ccaggcgtcg cgccagcttt gaagaagtgg tcggcggttt ggatacggac
     5221 acggctttgc tcgaagcccg acgctgtctc tcttgcggaa attgctttga gtgcgataac
     5281 tgttatggcg tgtgtccgga caacgccatc atcaaattgg gccccggtca acggtacgaa
     5341 gtaaattatg actattgcaa aggatgcggg gtgtgcgcgg aagaatgtcc ctgcggcgcc
     5401 atcgatatgg tgaaagaagt cagataggaa cagattatat atacgcacac cggagtttga
     5461 tcgttggaac aggtacgaag agttatttga cagcaataca cacggcacaa aggatgtgtc
     5521 acacccggga gacgatgtat gaaataccta ctagcagttg atggatcgga tcaatcggtc
     5581 gatgcgatac gtgcgtttga agcgctgagt ccggcggaga gcctgatgtt gttacatgtg
     5641 gtgaatgtgc ccggtatccc ctatccgtcc atcggggcgg ggatcgcgaa agatttgagg
     5701 ttggcggtgg acaaagcgat gagggaagaa ggcgaacgga tcattgagca ggcattgtcc
     5761 ctcttgcctc ttcatccggg atcggtcatg agacgactgg aaatgggtac tcctgccgaa
     5821 gtcattcttg ccatggtcaa ggagaaggga atcgatctcg tggtattggg cgccaggggt
     5881 attggtcaga ttcgagagca gatgttcggg agtgtttcgc atcgtgtgat gacacatgcg
     5941 tcgtgttcga ctctgatcat taagaagccc actcgaaaga tccagcaggt attaattcct
     6001 gttgaaagtc aggaggacgg tgaagtggtg gtgcggtttt tgaaaaagaa gccattccgg
     6061 gagccgatca ctgcaaccgt tttgcatgtc attccatttt cagaaccggt ctggccggtg
     6121 ggtgcgatga tttctccgga atttcgaaaa gagatggtcg cctatgggga gaaatttacg
     6181 aacgggctca gcgcggaatt gaagcagatg gggcatgagg ccaaaggggt tgtcgttgtc
     6241 ggggcaccct cacacacgat tattgaagaa gcgaataaat ttgctgctga tgtgatcctg
     6301 atgcggacac atagccgatc cggggtgagt cggtttcttt tggggagtgt ctcccattcc
     6361 gtggtgcatc acactgagtc ttcaattttg tttgtgcgtg aatgacccgg tctaaaggac
     6421 caatctggac aaactatccg tttccgaggt tgtatgcata aaacctggta ccttattggt
     6481 gcaattgcga taggcgtgtt agtttttctc tatctggtgt tttggtgtcc cagcccctgc
     6541 cagtaaggat aatcttgatt tcagtcgcac gtcctcctgc accccctggc gacacacttt
     6601 tttgtcgatt gtgcaacact gtccggatat actccccgat gaggtcgatc tggtcccttt
     6661 ggagtggtag actggaaact ggctccccgg tcctgccaaa tttttcggcc tgttcaggcc
     6721 acattcctga agaatctggg tctaggtatt ggggctagtg agttttttta catatttgtt
     6781 catatgaggc attccgtccc cacatataga gggtgttggg agggcagggg gcagtataag
     6841 ttggtggagc ctttgaggcg ctcaaaagct gacaggcgac aaaaaactag attgtccgtt
     6901 ataatataaa agggatgaaa taagagggaa attttctctt ttgagaagga tatcaataca
     6961 tgattaacaa atcggtcaga aaaatagtct cccttctttt aggtttcgcc ttgaccttta
     7021 atctggtgtg tgccaacaat ctttgggcta gtccgtccgt tcaccagatt ccgcatctga
     7081 tcattcccat cctgggaaca tctctcaata aacaatttag gccggtgggc atggtgagcc
     7141 aggtggtgat tgatgtctat gaacgccagg atcgcaatgg ccttcaaatc caatttcata
     7201 ccgaaccagg aaatttttct ttgtttgccc gtcagtcgat ccatgcagcc atcactcggg
     7261 cgctggctgc tgccaatctt ccgtcggaat cctggacagt cctcctgaaa tttccctata
     7321 acggcctcac agtgtacggg gaaagtcttt cggccatggt aggtctgagc gtagtgtcca
     7381 tgatcaaggg agatcatctc ctggaaggtc gggccctcac cggaacgatc acggaaagag
     7441 gatcgatcgg agcggtggga gggcttcaac tcaaaatcct agctgcctat gaaaatcact
     7501 ttgagcgtgt gttggttccc agcgagtatg atgtacggga tggagattgg cgtaccccgt
     7561 ttttgatgca ggtttcccct gttgggacgg tcgatgaagc ctactttggg ctgaccggtc
     7621 atcatttggc gacccttccc taagatttcc ctttcaattt tcctgcagtt gcccgggagg
     7681 atggattttg gcctgctgcc cagtttcttt gcgaaaacct cagggaaata tcccccaaat
     7741 tattaggtaa acattcttga aatttgccaa taggctgaaa ccctattggt tgaattggtc
     7801 tttctgcttc ttgccagttc ccttctgcaa ttctttatgg gaattatgtc atcgccggag
     7861 gaaggttttc tttggtcagt gttaaaaaat gtgttgcgca ggagatgcag ggatcataac
     7921 tgcgaatcaa ttcttcgcat ttcatcgcgg tttcctgatc gttgaggtga aggatgcctg
     7981 gcaggaggtt tcgaagatcg tcctccatgc ggcgttgatt ttgggaggtt ggcggcacaa
     8041 tcttggcgag tcgaatggtt ccatcagcgt tgacctcata acgatggtat aaaaggcctc
     8101 gcggcgcttc ggtcgcggcc atgccggttc cggcgcggag tgtcaatggt acgtctggag
     8161 caggtggccg ctcatactgg ttaatgagtc gaagggcctc gtcaaaggca tagagcactt
     8221 ccaccgatcg tgccacgata cccatggacg cgcggttgaa cgggaggact aatcccgtgc
     8281 tctgtaagac gtctttggcc aacaacgaaa gctggtcctg gttgagagcg agccgggcta
     8341 acggccctgt gagataagat tgtccattca ttgtgcagtg gagggcattg gaatagggca
     8401 cctgttcttc atggaaatgg tgttcaaact ccgatgcggc catgcggtgt ccccgattgg
     8461 tgacgaggtc gccttcattc atcggatatt cagaaggtcc ccggagggcg aggaaggtat
     8521 aggcctgttc gggatcttga aattcaaacc cgctcaccca ccgaagcgtt tccgcagcgg
     8581 catctctggc ccaaaggaat tcgtccctca gcccatgtaa ggcattcctg gtcggcactt
     8641 gggagaatcc gccgatcttc accgaaaccg gatgcaccga tcgtccccct aacagcgtta
     8701 acagatcatt gcccgccttt ttcattcgaa gccctcgctt cacaagatcg gggtgtgagc
     8761 gggccatctc aagcccgctg tcgaatccta agaaatccgg accctggagc atataaatgt
     8821 gcagaacatg actttcgatc cattccccgc aatacaggag tcgacgtaaa tcgcgaaggc
     8881 tttgtggcat ctccacatcg aagatgtgtt ccagggcatg gactgcactc atttgataag
     8941 ccaccggaca aataccgcaa atacgggcca cgatatcggg cacttcgtta acgtgccgtc
     9001 cctggaggaa ggcttcaaaa aaacgaggag gttcaaaaat ccggagttgc acatcccgga
     9061 cctgaccatc ctgaacggtg atatgaaggg ctccttcccc ttctaccctg gccatcatgt
     9121 cgattttgat ggttctggat tgatccgggt ttgttagagg atcgttcatt tgtctacctt
     9181 cgattttcaa tgagaaaagc caggaaaaat cctattctcc gggaatgatc aaaaaggtcc
     9241 atccagcaag gccgcacgca gtgaggagga cgaggcgtac taaccagtac gtcgagtcct
     9301 acgaacaagg agaacgccgc tgatggcccc ttcggtcaga ctcagggcat gctttttcaa
     9361 cattccctat ctggagtccc aggcatcgct ttccttccga aacggctgaa ccgatccgtt
     9421 gattcctcga aaacggcgaa ccatgtccag gggaatgagt tgcagatgct catgaaattg
     9481 tttggccaga gatccggtat ttggaggcac tccgggaatc actgaatcct gttcggcggg
     9541 gccaaagcac ccataacact cccggcccat agcggggcag atggccccgc acccggtacg
     9601 cgtcaccggt ccaagacagg ccagtccctt cgcgaccagg atgcagacat gtccctgtcg
     9661 tttacagtcc aggcatacac tgtgtgcggg aatgctgggt gtgacgcctc tcagcaaatc
     9721 ggtgagcact cgaagaagtt gtgacttatc aatcgggcat ccccacaatt catagtccac
     9781 cttgacgtgt tcggaaattg gagtcgatgt gcttaggctg tggatgtatt cggggctggg
     9841 gtataccgtt cgcttgaagg cttccacatc gccccagttc cgaagagcct gaatgccgcc
     9901 ggaagtcgca caggcaccga tggtgatcaa ggtggttgtc tgttgccgga gatcgagaac
     9961 tcgagaaaga tcttccggac aggtaatgga gccttcaacg aatgttatat catacgggcc
    10021 gggctgcata ttgctggagg cttcggggaa ataggcgatg tcgagggcct ctcccaacgc
    10081 cagtaaatcg tcctccatat tcagaatgtt taactggcat ccgtcacaag aggcaaactt
    10141 caccaccgca atccgggggc gaggcctggt tgaaggatgc tgagttggga tagttgttcc
    10201 gttcgtattc atcactggct attcaatatc ccctttttga acacgggaag cagggacgtt
    10261 tgcttttctt tttccgcgtc atccccgaca ttttcaatcg ggaatccatt tcttaaatca
    10321 gttcccctcc tttgtaagga ggggcgaggg gaggtagagt ttcccaggcg gaaagaagtt
    10381 ctcctactgc caccgatttt ccctttgaca attctacctc acctcaatcc tccccttaca
    10441 aaggggagga aatatggttt tgagtttctc ctacaccccc actttcccaa aaaacggcac
    10501 cacccggtct aatcgcatca cgggcccgtc cttacagaca aagaacggcc cgagctgaca
    10561 atgcccgcaa agtccgatgc cgcattccat atgccgctcc aacgacacat acatggcgtc
    10621 agggcgcaga ccctgatgag ttaaggtatt acaggcaatc cgcatcatga tctccggtcc
    10681 gcacatcatg accagcgtat tgtcagggtc caactgcagt tggtcaaaca attcggtgac
    10741 cactcccaca tggctatgcc aggtcttgcc gggttcatcg ctggtcaata gtacctgtgt
    10801 gcggggttgg tggcgccagg catcgaagcg ttcccggtag agcaagtcgt ggggggtttt
    10861 taccccgtgg agaatggtca tggcgccata ctcctcccgc ctgcggaaca tatattcaat
    10921 ggctcccacc accggggcac agcccaatcc gccggtgacg ataaccacgt cctttccttg
    10981 cgcttccttc atgggccatc caatcccaaa cggcccacga acgcccacac tctcacccac
    11041 ttgcatcgcg cccaatacct gcgtgatccg tcccaccaca cgaatggtgt ggtccagaaa
    11101 ttccggctca ccagggtccg agacaatgga aatggctacc tcgcccacac caaacacata
    11161 gagcatgttg aactgccccg cctcaaaccg gaatgacttc cggtgatcag ggtcgttgag
    11221 ttgcagccga aaggtgacga tgtcggtgga ctcctgtttc ttgtccacga tgactgcagg
    11281 gtgaatgagg tagggattat gagacatgcc gtagccgagt ttttagtgat taatttacca
    11341 aattaccagt taaggaaact catcagtcat cataaacaat tcaatcactc agatcttggt
    11401 tagtgaagac tcctcatcat gaaaactaat gagtggtgtg gcgtttttcc tcaaaacgta
    11461 aagattggac acccctttgg gtataacagc aaattcaaag ccagaatttt tgtagttatc
    11521 cagactgaag ctttttaaaa ttttaatagc ttttagaatt gacagaagag agatcaatat
    11581 agcttcatat cgcatcttag gttttttttc atagataatt cgtccagcta tcggttcaag
    11641 tcaaatagtt aaaaacaaaa ccaaaaatga ttcttatcac caaagattgg gagcatatgt
    11701 attgtacgaa aggttgtgtt ccaaagaaaa tgaatggagg gaaaatgact aatgatattg
    11761 gccgaccgtc tgtacaccga aactgacgac acttgtgcac tctgtggact ccgtgatacc
    11821 caggcgctaa ccattcacca cattgacggg aaccatggaa acgacgtcta cgaaaacaca
    11881 atcgttctct gccacaactg ccatattcgg tatcatcaaa tgaaaggaat tacagaagaa
    11941 gacataagga cacgtaagcg gcatcttatg tataagacga tcactaccta tggcgtgaac
    12001 gctctaaaaa tcgctgcccg gaacaatttt ggtgtcatcg cgatgccatt tttgctatat
    12061 cacttggtcg gtcttggcta tatggataag aaagaggatc aaatgggcta cggtgctcaa
    12121 tcggacgcaa cagcaaggtt tgcgataaca gatcgaggcc gtaatacttt gagagaatgg
    12181 tttgagtaac aataagaagg gaaagcgacg ggaatattta tctttatttt tagttaaaaa
    12241 cgaggttcga aggactcacc tatcagaatt agacacaatt ctggattaaa tagtgaggta
    12301 atgaaattaa tggaaaaata gtgttccttc tgatgagcgt cgaaaaatat cctaatctga
    12361 acagggcgac cggattatgg tttggtgagg ccgtgcactt tgtcaaaaaa gtcgaagagg
    12421 ctggccggca tcgggctgtg ctgtagcgga tctcctgatt gctgcacttc gaagaaactg
    12481 accattcagg aattatgatt ctgattttag ttttaggcgc tgtgtatcgg aaatgtcgtt
    12541 tacgaattgc ccgtaataaa gcatttcagg tgggctcctc gaatttgtcc cccaactcta
    12601 gtgtcgaggt gattttctgt gttgctgggt ttcgccccag tagacgaggc ccttttgttc
    12661 cggcaaaagg gcccaaaacc attgacgccc cgtctggtct cattagaacg gatggacgct
    12721 agtctcaagc gggcggacca actcgctccg ctcaaacaag gcccgccagg gaatgagagc
    12781 gtccctcctt aggccagacg gcaggcgtct gaccatggaa gacgaacctt tcagagatac
    12841 acatgaaata gccgttaacc ttgttcgttc tgctctgggg gaaatcccat gcaatatgtc
    12901 acacctaaga aatttgctgt tcctctgaat cgccaagaag tatctcagtc atggaatcag
    12961 cgggggtatt cgtgcgatgt ttttattgat cctcccgggc gggagtggaa tgatttcgtt
    13021 cattcgacca acgagttggt catcgttgtt gaagggaaat tgaggatgac cattgagggt
    13081 gaagagatta tcgtggaacc cggagatgag gtattcattc cgagagaggc ctgtcattcg
    13141 gtaaaaaata ttcatcacgc gacgaccaaa tggttgtacg ggtatgattg agagcatgaa
    13201 agtattaatg ccatccgtta gaacttggtg cgaagtgatg aatgggtcat gccacacaga
    13261 cgttctggga ggcgatgaaa ttcttatggg gacaaatact tcgccgccgg ttcaggatga
    13321 ccatatgatg aagggataac cgaatcatta tttgttcgtc caaggtttgg gtggtgaaat
    13381 ccgggttttt acattttttt ccatatgtcc tattctatga tccatgatgc gatttatccg
    13441 tctgtccgac tacacctggt ggatctggtt gtgcctcgcc ggttgcttac tggccgggct
    13501 gctagttgac ccgacttact tctttctggc tatcctcatt tccaccatcc aagccttggt
    13561 tttttttgct cgcgaagaaa atgccttgtc ttttcctgcg caacttcgtc tcgcgtatct
    13621 gcttctcctc gtcgtgctgt atctgccgcc acttcgattt ctctattggg ttccggctat
    13681 tggcacattg atcctgtgtt ttaccgggta ctgtctgctg gcacgtggtc tttccttgct
    13741 cccatggaac cgggactccc ctctgacgtt tcaggaagtc atcaggacct tcacggttcc
    13801 tcctaatctt gatgtcacgt tgcgaggcaa accggcatcc gggtgtcccg gtggagtgtg
    13861 ttcactcgaa gtgcaaatcg gcaagtccgc aaaggcctga agagtcgatc aacgatttgt
    13921 tagggtaccg gggaaatgta tatgtggatg gagaagcgct tagtgggata tcgtatttcg
    13981 aattggggat tccttctgaa ttggtgttga tgacgttgaa aggacaacgt agccatcctg
    14041 aaggctgcat gggcgggctg tgataattta gagatcatgc ccatctcaaa aaagtctggt
    14101 gcctggaatt cacatcattg gacgtaggtt caggtcccga ttccgatggc ttatttcggt
    14161 tagaggtttt gtatgcagta ttcattcact ttacacaaca taggagaaga atatggcgag
    14221 tgttggttat cacgaaccca ttgaggaatt atccgatgaa acgcgcgaca tgcatcgagc
    14281 catcgtttcg ttgatggagg aactggaggc tgttgattgg tataatcagc gtactaatgc
    14341 gtgcaaagac ccggacctca aagccatttt ggcgcacaat cgcgacgagg agaaggaaca
    14401 tgcctcgatg gtgcttgaat ggattcgacg caaggatcct acattttcga agcatttgaa
    14461 ggactatctg tttacagaga aacccatcgc gcataagtag ccgggcgatg tcgattggtg
    14521 cgacctctgc caggcttcac tgccgcttgg cttattcggg tggctggcag agggcgtaga
    14581 cttcctcggt gagatcgatg ccccatcgga taccagttgg tggcctcttc aacatttaaa
    14641 caatggaagt tttgggatag aacctccatt aagctctaag cgctttgacg ttataacgct
    14701 aaagcgctat agcgccagta tcataaggag gtgaaattcc atgggcacaa ggtctaaacg
    14761 atcgacagtc tactttgatg agaatctcca tgcggcgctc agacttaagg ctgcgcatac
    14821 tcaccgatcc gtatctgaca tcgtcaatga tgctgtgcga gcagctctcg cggag
//
LOCUS       DATIAL010000029         5350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_650776, whole genome shotgun sequence.
ACCESSION   DATIAL010000029 DATIAL010000000
VERSION     DATIAL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650776"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VLA60_01070"
     CDS             complement(<1..782)
                     /locus_tag="VLA60_01070"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF07972.1"
                     /translation="MKSATSTGHTNPASFLETLLAEAPETLPILRQSCIQVLNLTQDK
                     RSNASDIGEIIMRDQAMMANVIKIANSPAYHTRTPVKTPTYAVALIGFDVIRAMVVSA
                     QLIEQADTFGADTTNLKHLLARALVAGTQAQELGKAINYGETGSLFTNAMLYSLGDLI
                     LALCRPDVAEQLEVIRREDPAKIPKAELTLLGRPLHVIAAAMAKHWKLPDTLVQLLEK
                     KPVWPKSRPEADQQIMEGIVRAANELSYCLLNPLAAGQGEVL"
     gene            968..1729
                     /locus_tag="VLA60_01075"
     CDS             968..1729
                     /locus_tag="VLA60_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07973.1"
                     /translation="MRNRISCSAQELSQLDADMIQSVVGGTVFEEGHQYFSAQRVKIL
                     DADQTQITAEVHGVYGVYTQIIKLRAGTLSTRCSCPSTEQPFCRHCVAVLLHQFHNGS
                     SLKPSAKENHKDPAPASDLRGRAGAPHAEESGGAGDLNFWEAILFIDWVQKAVGLLGK
                     EATLPPVPGSLGGVAREWVSVVERLNAQCLEGEEDRLDALRSLQSAEGMVDNLTKKLQ
                     VLKEEAEVAQKNCRVLEEKVKQLQNSLAELSKASN"
     gene            complement(1879..2808)
                     /locus_tag="VLA60_01080"
     CDS             complement(1879..2808)
                     /locus_tag="VLA60_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HSF07974.1"
                     /translation="MPCIVSVASGKGGVGKSMVVSNLGLLLAKKGLRVTLVDMDIGGA
                     NLHILFGMFHPPSTLTDFLTNTLTDLNEIAHPIPSLSSLKLIPGTGETLITANLQHAK
                     KRRLIRHVQKLEADIILVDVGAGTSYHALDFFLLADYYLAVATPDPTSVLDLYRFIKL
                     AAIRKVLTAFLARDPVANALLDKEFHSVTAVLEAVGKTSESSVGIAQEALKMFQPALI
                     LNRMTPKSRINTLHLQHLLKQYVGTDLSILGNIPEDIHVQQSILKYLPVVELAPTSPA
                     TIALNQIADNVLGMVGRTDGSIDRREEPRKIRA"
     gene            3080..3559
                     /gene="rimP"
                     /locus_tag="VLA60_01085"
     CDS             3080..3559
                     /gene="rimP"
                     /locus_tag="VLA60_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HSF07975.1"
                     /translation="MAFTADNLEQVIRQVGEPIARALGVDIFEVQCTGRPANLLVRLT
                     LDKKGGVGIEDCEQFHQSLRRTWEVLHPEQAAYRFEVSSPGLDRPLRGPKDYERVVGE
                     RLRVTLKTSINKQSVVLGQLITVTDMGIHLMDDKSKNPQEVVVPWEDIAKARLEVSF"
     gene            3632..4840
                     /gene="nusA"
                     /locus_tag="VLA60_01090"
     CDS             3632..4840
                     /gene="nusA"
                     /locus_tag="VLA60_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HSF07976.1"
                     /translation="MKSELMLVIDQIGREKGIDKAKVILALESALLTAAKKRFGHGEN
                     IQVDIDIETGEISIVKKKTIAENVLDSKTEISLEDARKIDDEAEMGDEIGSLIDLEEF
                     GRIAAQAAKQVICQKVREAEWESIEREYSKKEGELVHGVILGQERRNYLVDIGKTEAL
                     LPIQEQIPREAHRRGDRVRALLLEVRRTPKDVQVILSRAHPQFVVKLFGLEVPEITEK
                     IVEIKGVVREPGDRTKISVASRDKAVDPVGACVGVKGSRVQAIVRELHGEKIDIIPWT
                     NDPRVFIGEALNPASIEKVGIDEQKKSALVVVADSQLSLAIGKNGQNVRLAAKLTGWK
                     IDIISSTEYEKQKLERDQEIKAALAEEAAQITEEPAADNAKEIERSSQDEIVENPAVV
                     PTESGQSSGS"
     gene            4869..>5350
                     /locus_tag="VLA60_01095"
     CDS             4869..>5350
                     /locus_tag="VLA60_01095"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HSF07977.1"
                     /translation="MRVHEIAKKIGMESRLLIPELVRLGIQVSSHSNTVEDNMAKWAM
                     NIILGKTPETTPKPGESSVSGPVGVKSSEGGRLLKKESSTSGRHRSEAVAEEPKKAEK
                     KHILIKKKKTEEEILEEMAELSPVAEGEGEESPVIQEESESPVEAASVLTQSSGLSAV
                     "
BASE COUNT         1392 a         1130 c         1512 g         1316 t
ORIGIN      
        1 tggagtacct ctccctgtcc ggcggccaat gggttcagga ggcaataact caattcattc
       61 gcggctcgaa caataccttc catgatctgt tgatctgctt cgggacggga tttgggccag
      121 acgggctttt tctccaatag ctgaaccagg gtgtccggca gtttccaatg cttggccatg
      181 gcggcagcaa tgacatgcag cggtcttccc aaaagagtta gttcggcttt tgggatcttg
      241 gctggatcct cccggcgtat gacttctagt tgttcggcga cgtcgggtcg acagagggcg
      301 aggatgaggt ctcccagtga gtagagcatg gcattggtaa ataacgatcc cgtctccccg
      361 taattgatgg ctttccccag ttcttgtgcc tgggtccccg cgaccagagc gcgagccagt
      421 aggtgcttca gattggtggt atcggctcca aaagtatccg cttgttcaat taattgggct
      481 gagaccacca tggcccgaat gacgtcaaac ccgataaggg ccaccgcata ggttggggtt
      541 ttgactgggg tgcgggtatg ataggcaggg ctattcgcga ttttaatgac attggccatc
      601 atggcttgat cacgcatgat gatttctcca atatcgctgg catttgagcg tttatcctga
      661 gtaagattta ggacttggat gcaactttgc ctgaggatcg gcaatgtttc cggggcttcg
      721 gcgagcagag tttctaaaaa ggaagcaggg ttcgtgtggc cggtggaggt ggcggatttc
      781 atgatggcac gtccggcagg gtagagtgcc aggcggtaat tcaaggacgt ggtaatttta
      841 aatcattgca atggaatgtg gtaattggct ctcaggttaa ttctcggtaa tattttccga
      901 aacattaaac cgtccttacc ctttgaggtg aaggcgggac tcattttttt gattgagtgt
      961 tacctgtatg agaaatagaa tttcctgctc ggctcaggag ctgagccaat tagatgctga
     1021 catgattcaa tccgtggttg ggggaacggt cttcgaagaa ggccatcaat atttttccgc
     1081 ccaacgagtc aagatccttg atgcggacca gacgcaaatc acggctgagg tccatggcgt
     1141 ctatggcgtg tatacgcaaa tcattaagtt gcgagcggga accttgtcga ccaggtgttc
     1201 ctgcccatcg actgagcaac ctttttgtcg ccactgtgtt gcggtattac tgcaccaatt
     1261 ccataacggt tcctcactca agccgagtgc caaggaaaat cataaggatc cggctcctgc
     1321 ttccgatctg cggggacggg ccggagcacc ccatgcggag gaatccgggg gtgcgggtga
     1381 tttgaatttt tgggaagcca tcctatttat cgactgggta cagaaagccg ttggactgtt
     1441 aggtaaggag gccactctac ctccagttcc cggttcgctc gggggggttg ctcgagaatg
     1501 ggtaagtgtg gtcgaacgcc ttaatgcaca atgcctggaa ggagaggaag atcgacttga
     1561 tgcgttgagg agcctgcaat cagccgaagg catggttgat aacctcacga agaagttgca
     1621 agttttaaag gaggaagccg aagtggctca aaagaattgc agggtgttag aggaaaaagt
     1681 caaacaattg caaaattcat tggcagaact ctctaaggca tccaactagg tcaggcaatt
     1741 tgcggcaggt ttggtgggca atgtgacttt aaagttaagg cgatgatcag tgaaacgtga
     1801 ggggagtctt ccgacccttt ccgtacgagc ccgcaagttt tttcaaaaaa agccaaggcc
     1861 atgacgtcag taaatcattc atgctcggat ctttctgggc tcttccctcc ggtcaattga
     1921 tccatctgtt cgccccacca tcccaagaac attgtcggca atttggttca gcgcaatggt
     1981 cgctggggaa gtgggagcta gttctaccac tggaagatat tttaaaatgg attgttgcac
     2041 gtgaatgtct tccgggatat ttcctaagat ggaaaggtcg gttccaacat attgcttaag
     2101 cagatgttgt aagtgcaggg tattgatcct tgatttggga gtcatgcgat ttaagataag
     2161 ggccggttga aacattttta aagcttcttg ggcaatcccc acacttgatt cgctcgtttt
     2221 tccaacggct tccaggactg ctgtgacgct atggaactct ttgtccagaa gggcgttcgc
     2281 cactgggtcg cgggctaaaa aagccgtaag aacctttcga atggcggcaa gtttaatgaa
     2341 tcgatacaga tctaggaccg aagtcgggtc aggagtggcc acagccaggt aatagtccgc
     2401 gaggagaaaa aagtccagtg cgtgataact ggtgcccgcg ccgacgtcaa ccagaataat
     2461 atctgcctcc agcttctgta cgtgccgaat aaggcgtctt tttttggcat gttgcaagtt
     2521 tgcggtaatc agcgtttccc cggtacccgg aatgagcttc agggaggaga ggctgggaat
     2581 agggtgggcg atctcattta aatcagtaag cgtgttggtc aaaaaatctg tgagagtgga
     2641 tggaggatgg aacatcccga ataaaatgtg gagattagct cccccaatgt ccatgtcgac
     2701 cagcgtgacg cgaagcccct ttttggcgag gaggaggcca aggttgctga cgaccatgct
     2761 ttttccaact ccgcccttac cggaggccac tgaaactata caaggcatcg gttgaactcc
     2821 tctctcgtag atttcacctg gaatcgcgtg ccaccctact cagaaaatag cttatcatgg
     2881 ggcccgtctt atgcccatgg tacgtgacta tgctgggggt tgaagagatg gaggatttcc
     2941 actcagctag ggcgttgctg tgaatcgggg aagctcttgc ctttaaaaat ggaaagtgct
     3001 aggttatttt cgagtagagt gggcgtgagc ccactttttt ttggtcttgg tttgtggccg
     3061 ctcgtagtga ggaattgtgg tggcctttac ggctgacaat cttgagcaag ttattcgcca
     3121 agttggcgaa cctattgctc gagccttagg agtagatatc ttcgaggttc agtgcaccgg
     3181 gagacccgcc aaccttttgg ttcgattaac tcttgataaa aaggggggag ttggaattga
     3241 agattgtgaa caatttcatc aatctcttcg tcgaacctgg gaggttcttc acccagagca
     3301 agccgcgtat cggtttgaag tttcttcgcc tggtctagat cgaccgctcc gaggtccaaa
     3361 agattacgaa cgagtggtgg gcgaacgact tcgagtcact ctaaaaacct cgatcaacaa
     3421 gcaatcagtt gtcttggggc aattgattac tgttacagac atggggattc atttgatgga
     3481 tgacaaaagc aagaatccac aggaagtagt tgtgccctgg gaagatattg ccaaagcgag
     3541 attagaggtt tcgttctaac gttttactga aaatgcgcac caatcgccca tgtccgtctg
     3601 aaccgctcat cgtgccttgc gaggaataca tatgaaaagt gaactcatgt tggtcattga
     3661 tcaaatcggt cgggagaaag gaatcgataa ggccaaagtc attttggcct tggagtcagc
     3721 gcttctcacg gccgcgaaga aacggtttgg gcatggtgaa aatattcaag tcgacattga
     3781 tatcgaaacc ggcgaaattt ctatcgtgaa gaaaaagacg attgcggaga acgtgctgga
     3841 ttctaaaaca gagatttcat tggaagatgc caggaaaatt gatgatgaag ctgaaatggg
     3901 agacgaaatt ggctcactca ttgatttgga agagttcggg cgtatagctg cacaggccgc
     3961 gaagcaggtc atttgtcaaa aagttcgaga ggctgaatgg gaatcgattg aacgggaata
     4021 ttccaaaaaa gaaggggagc tagttcatgg ggtaattttg ggccaagagc gtcgaaatta
     4081 cctggtcgac attggaaaaa cggaggccct tctacccatt caggagcaaa tcccacggga
     4141 agcccatagg cggggcgatc gggtgcgggc tttgctgctg gaagttcgac gaactccaaa
     4201 ggacgtacaa gtgatccttt cccgagcgca tcctcaattc gtggtgaaat tgtttggtct
     4261 ggaagtgcct gaaattacag agaaaattgt tgaaataaaa ggggtagtac gtgagccggg
     4321 tgaccggacg aagatttcag tggcttcacg ggataaggcg gttgatccgg ttggcgcatg
     4381 cgttggcgtc aaagggtcac gtgttcaggc cattgtccgc gaactgcatg gcgagaaaat
     4441 agatatcatt ccatggacaa atgatcctcg ggtttttatc ggtgaagcct taaaccctgc
     4501 ctccattgaa aaagtaggga ttgacgaaca aaagaaatct gccttggtgg tggtggccga
     4561 ttctcagctt tctttggcca taggaaagaa tgggcaaaac gtacgattgg cagccaaatt
     4621 gacaggttgg aagattgata tcattagctc cacagaatac gaaaaacaaa agcttgaacg
     4681 agaccaggag attaaagccg ctttggctga agaggctgct caaattacgg aagagccggc
     4741 ggcagacaac gccaaggaaa ttgaaagatc ctcacaggat gaaatcgtgg agaatccagc
     4801 ggtggtacca acagagtcag ggcagagctc gggatcgtaa ggatacggct tcacagtaaa
     4861 ggacgtgcat gagagttcat gagatagcca aaaaaattgg gatggagagc cgtttgctta
     4921 ttcctgaatt ggtgcgactt ggcattcagg ttagttccca tagtaatacc gtggaagaca
     4981 atatggccaa atgggcgatg aatattattc ttggtaaaac tcctgaaaca acgcccaagc
     5041 ctggggaatc atccgtttca gggcctgttg gtgtaaaaag ctctgagggg gggcgcctac
     5101 tgaaaaaaga atcgtcgact tctggaaggc atcggtctga ggcagtggcc gaagaaccta
     5161 aaaaagccga aaaaaagcac atactgatta agaagaaaaa aacggaagaa gaaatcctgg
     5221 aagagatggc ggaattgtct ccagtcgcgg aaggtgaggg agaagagtca ccggtcatcc
     5281 aggaggagtc agaatctcca gtggaggcgg cttccgtttt gactcagagt tctggcctat
     5341 cggccgtaga
//
LOCUS       DATIAL010000030         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_328441, whole genome shotgun sequence.
ACCESSION   DATIAL010000030 DATIAL010000000
VERSION     DATIAL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328441"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VLA60_01100"
     CDS             complement(<1..487)
                     /locus_tag="VLA60_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HSF07978.1"
                     /translation="MMHMSRLFAGTARPFRIAPPWGFLVLFVGLGMVGGFLGNGWFVP
                     AQTQAAIPGAFIEGFSEIVEKVGPAVVNVAVTGGGGPSRGLPPGPFGGPPGGGPPGGG
                     PGGPPGGGPGGPPGMSAGSGVIIDSRGYILTNNHVVEDASDIKVSFHDGREMSATVVG
                     TD"
     gene            complement(905..1261)
                     /locus_tag="VLA60_01105"
     CDS             complement(905..1261)
                     /locus_tag="VLA60_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07979.1"
                     /translation="MMIAGMASALSGIQAGGRMLSVGAHNIANAQTESFKRTRALPEE
                     SSAGGGVRVTLQTDERPGPQFLSSENSFSLREGSNVDLGEEIISNLQAVNLIEANMAS
                     MRIQEKVLCSLLDITE"
     gene            1501..2454
                     /locus_tag="VLA60_01110"
     CDS             1501..2454
                     /locus_tag="VLA60_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSF07980.1"
                     /translation="MRGLVLHQKLQLHTDLPLPPRPHGEARIRVIQAGICSTDLQLLK
                     GYMAFQGVLGHEFVGIVDEASDTELIGKRVVGEINAACRTCETCAKGHPTHCPHRTTL
                     GIQGRDGAFADFLTLPVENLFVVPDSISDDQAVFIEPLAAACEIPQIVSIKPTDRVVV
                     IGDGKLGVLCAQILVLSGCAVTLLGHHSDHDTWLTHMGIKVTSHPADIPQGADMIVEA
                     TGSPEGLSTAIQLIRPRGILVLKSTYHGDVSLNMATFVIQEISLIGSRCGPFPPAIRL
                     LESKHIRVDPLIHARYPLLDGVLAMEKAAQPGVRKVLLQMT"
     gene            2543..2618
                     /locus_tag="VLA60_01115"
     tRNA            2543..2618
                     /locus_tag="VLA60_01115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2576..2578,aa:Thr,seq:tgt)
BASE COUNT          835 a          843 c          697 g          803 t
ORIGIN      
        1 gatcagtccc gacaaccgtt gctgacatct ctcgtccatc atgaaaagac accttgatgt
       61 cactcgcatc ttccacaacg tggttattcg tcagaatata cccgcgagaa tcaataatca
      121 ctccggaccc ggcactcata cctggaggtc caccgggacc accaccggga ggtccgccag
      181 gaccaccacc agggggtcct ccgccaggag gcccaccaaa cggtccggga ggtaagcctc
      241 tagatggtcc acccccacca gtcaccgcca cgttgacaac cgccggaccg actttttcaa
      301 caatttctga aaaaccttca ataaatgctc cgggtatagc cgcttgcgtt tgggcgggga
      361 caaaccaccc gtttcccaaa aatccaccga ccattcccaa tcctacaaat aggacaagga
      421 aaccccatgg aggggcaatt cgaaacggcc tggcagtgcc agcaaacaac cgtgacatgt
      481 gcatcatccc gatctcctgt tcaaaaaatg taatagaaac ggagtgaata ggaaaatttc
      541 tatctcacaa cgctgggtgt tctcaagaaa ttccccaaga caaatcgcct cacttcgtat
      601 aaccgtttga ttctacaata attgttttcc cacgttcaaa gtcaaatcct tagtttttta
      661 ctgccacccg tacaggcgca tgacctgcag gttctcgtcc gtcttgtcaa gactttcatt
      721 ctaataaaga aaaaactttt ttgaaggaca taaacgtctt cagaaccggc tgtttggatt
      781 gataaagata caagaatgcg tcgctctgct gagcgaaatg cccatgaaat tactgccgga
      841 aaccttaaga cggagcaacg aagtggggat cgaatgaaga atgattacat tcagacagga
      901 ggtattactc ggtaatgtcc aagagggagc acaaaacttt ctcctgaatt ctcatgctgg
      961 ccatattggc ttctatgaga ttcaccgctt gcaggttgga aattatttct tcgccgagat
     1021 ccacattaga tccttcgcga agactgaacg aattttcact ggagagaaat tggggtccgg
     1081 ggcgttcatc tgtttgcaaa gtgaccctca ccccaccacc ggctgacgat tcctctggaa
     1141 gagcacgggt tcgtttaaaa ctctctgttt gggcattggc aatattatgc gcaccgacgc
     1201 taagcattct tccccctgct tgaattccag aaagagcaga ggccataccg gcaatcatca
     1261 tgacttctcc tttgatacga tagccatctc acaaccctcc atactccttc acgttatcga
     1321 ccaaagtctc aaaacctttc ccgacgagag tggaagagtt tccccaaaac gccggagata
     1381 gagatactct tcctgtggaa aattgactta ctgaatattc atagaaattg ctgaagattg
     1441 acagcatttt tgctatggtt ctatcaggcc aaatcctaag gtatttcatg aaggagccat
     1501 atgcggggac ttgttcttca tcagaagctt caacttcata ccgacctacc ccttccccct
     1561 cgtccacatg gggaagccag gatcagggtc atccaagctg gaatctgctc taccgatctt
     1621 cagctactca aagggtatat ggcctttcaa ggtgtcctgg ggcatgaatt tgttgggata
     1681 gtcgatgagg cctccgatac agaattgatt ggaaaacgag tcgtcggaga gatcaatgcc
     1741 gcctgtcgca cttgtgaaac ctgtgccaaa ggccatccta cccattgccc tcatcgcacc
     1801 acattaggca tccagggtcg ggatggggcc tttgcggatt ttcttactct gccggttgaa
     1861 aacctttttg tcgtgcctga ctccatttct gatgatcagg ccgtctttat cgaaccctta
     1921 gccgcggcct gtgaaattcc ccaaatagtc tcgattaagc caacagaccg ggtggtcgtc
     1981 atcggagatg ggaagttggg agtactgtgc gcacaaattc ttgtgctttc aggatgtgcc
     2041 gttactctat tgggtcacca ttcagaccat gacacctggc tcacccatat gggcatcaag
     2101 gtgacctcgc atccggctga tattcctcaa ggcgcagata tgattgtgga agccactgga
     2161 agccccgaag gactttccac ggccatacaa ttaattcggc ccagaggtat tcttgtgctc
     2221 aaatccacgt atcatggaga cgtgtcttta aatatggcga cttttgtcat tcaggaaatt
     2281 tctttaattg gatctcgttg tggcccgttc cctccggcca tccgtctatt agaatcgaag
     2341 cacatccggg tcgacccgct tatccatgcc cgctatccac tcctggatgg cgttctggcc
     2401 atggaaaagg ccgcacaacc tggcgtacga aaagtcctgc tgcaaatgac ctaaatcctg
     2461 gcaagttgga aaaaatattt ttatgtattc aatactggtt gaatggttca gcctttggta
     2521 tgataatgtc taaattcgcc gagctggcgt agctcaatcg gtagagcagc ggttttgtaa
     2581 accgcaggtc ggaggttcaa ttcctctcgc cagctccata caacacatgt gactgccaag
     2641 tttcggcagg atcgcaaaat taaaaggtat cgggaagacc ccaccgactg ttctgttata
     2701 gattcaatcg ttccctccca cccttccaac cgtgttcatc gcgacctctg cgtttaaaaa
     2761 cattttcata cgtcagaggt catcgctcac gttctaagct ttgaaggtca tgatacaagg
     2821 cctcttttct tccaaaaggc tatgggttca accgtttgtc caaattcacg cccgcgagcc
     2881 cttgtgtaat ccttattgaa tttttacatt aagaaaaact gcttttgttc agggagttat
     2941 ctgttaaaat agcttgacat gacactaggt cggtcccgca aggaattgtg agaggccccc
     3001 actccatcat gaggggagaa atgccaccct ttagcaggac gaacagtcaa agaataatga
     3061 ggaatattcc caacaggtta cccatctctt atcgcatgtg catgctcgga tcgcgcaccc
     3121 aatgaacgac gataagcaaa caaccctgcc tgcggaaaaa tcttccccca cacccctg
//
LOCUS       DATIAL010000031         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_543333, whole genome shotgun sequence.
ACCESSION   DATIAL010000031 DATIAL010000000
VERSION     DATIAL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543333"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /gene="mscL"
                     /locus_tag="VLA60_01120"
     CDS             complement(<1..244)
                     /gene="mscL"
                     /locus_tag="VLA60_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HSF07981.1"
                     /translation="MLKEFKEFAMRGNVIDMAVGVIVGGAFGTIAKSLVADVLMPPIG
                     LLLGGVDFSNLFVTLKDGVPPGPYLALSDAQTAGAVT"
     gene            complement(584..1180)
                     /locus_tag="VLA60_01125"
     CDS             complement(584..1180)
                     /locus_tag="VLA60_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07982.1"
                     /translation="MDEIHQKSVSQMEDGGLSERQERMVPIPHWYSDMMIIEQRTLKE
                     TCLEQTFLRSLREGRDLMGTAKNKVLQSSPRLGRVLIVTRDHLIRDFLGQIIKLHGYD
                     CEYLEELPKAIMEQTWKDFDALFIESHSLEQLKRGMRAGISRASVSPMVVVLGDLPPS
                     DEPGHFRELKKPLDYRQMGRLMDECLFLKTQRCALQGD"
     gene            complement(1783..2850)
                     /locus_tag="VLA60_01130"
     CDS             complement(1783..2850)
                     /locus_tag="VLA60_01130"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HSF07983.1"
                     /translation="MSRRLMLCCAYLILYLMVLPVICLAGPMGKITLKDGSQVYGEIV
                     EMTEGKIQIKTLFHEGKDPVTIKWSEVTGIDTEDPMSFVLSNGTILQGKPAMTKPGTL
                     NVKTELLPEPIAVPLDSILAVNPPAKKAVAFTGNFNFGGSVTSGNTDVKNFSFVGELV
                     ARSERLRLSTLGRYLYGENDGQLNARNAYGTVKMDFFVTKRFYMYTGVLFEQDTFQDL
                     NLRSSVFAGPGYQIIDEGDYDSPYLSKLQLHGEVGLGYFNEDFKRADDVNYFTGRWAV
                     NLNWPILPNLTLFHQHQGFPSLDKTSDWYVTSQQGIRMNIWENFITTFQVNWRYDNSP
                     APGTKKADTQYILSLGYAFDA"
     gene            complement(3665..3877)
                     /locus_tag="VLA60_01135"
     CDS             complement(3665..3877)
                     /locus_tag="VLA60_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07984.1"
                     /translation="MNHDKTSEEANDSETAGDKDLKAVSPKKIKKELSVVSLPHDNDV
                     MAQEMADLFDEDRVTHKHGNAEPEKD"
     gene            complement(3901..>4208)
                     /locus_tag="VLA60_01140"
     CDS             complement(3901..>4208)
                     /locus_tag="VLA60_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07985.1"
                     /translation="PICKANRFGIDSRTATGDGEWKAICIGCHYMFPVHTDMEFYVQT
                     QPDIPYHLKEIPCPSCRHRGVSLDFRAVLSVRESVYFVTCPSCQLKFPERSHLESFE"
BASE COUNT         1179 a         1079 c          874 g         1076 t
ORIGIN      
        1 tggtgacagc gcccgctgtc tgagcgtcgg acaaggcaag atatggccct ggaggcacac
       61 catccttaag ggtgacaaac aggttggaaa aatcaacgcc acccaacaaa agtccaatag
      121 ggggcatcaa cacatcggcc accagcgatt tggcaatggt gccaaaggcc cctccaacaa
      181 tgacaccgac tgccatatcg ataacattgc ctcgcatggc aaactctttg aactctttaa
      241 gcatcacatc ctccttatca tgagcgtgaa cactagaacc ggttgttttc caggatgatc
      301 aaaaaaaggc tgcatcatcc tctctcaaca caaaaacaaa tgcaagatta ttccagagct
      361 tcccgttctt cgcctaaccg ggctttcacc tccaatattt tctgcaatcc ctgaaaccac
      421 aggaccggcg acaggcaaaa aaacctgggg cggccggcct attctcgcct atttccagtt
      481 gaagaccccc tctaaaagcc ccaactgtca agatcagctc gaataggtta gccgccctgc
      541 gtgttccgca cccgacaaac agtctaccat tacgatccac cttctaatcg ccttgtaagg
      601 cgcatctttg cgttttcaaa aaaagacatt catccattaa tcttcccatc tgacgataat
      661 ccaagggttt tttcagctca cgaaaatgcc ccggttcatc cgatggagga agatctccca
      721 ataccaccac catcggagaa actgaggcac gggagattcc agccctcatg ccacgcttca
      781 gttgttccag agagtggctt tcaatgaata gtgcatcaaa atccttccaa gtttgctcca
      841 ttatggcctt tggcaattcc tcaaggtatt cgcagtcata cccgtgtagt tttattattt
      901 gtcccaggaa atcacgaatt aaatgatccc tcgtgacgat caagacgcga cctagcctgg
      961 ggctcgactg aagaacctta tttttcgccg ttcccatcag gtctcttcct tcccggaggc
     1021 tgcgcaaaaa agtttgttct agacacgtct ctttcagagt tcgttgctcg ataatcatca
     1081 tgtctgaata ccaatgtgga ataggcacca ttctctcctg gcgttcagac aggcccccat
     1141 cttccatttg actcactgat ttctgatgga tctcgtccat tcctaatcca gcaggaagct
     1201 tctctttgcc aaagaacatt tgagtccgat gcgaatgccg tgagggagaa cagatccctc
     1261 gacacctccg gaatataact cctgaccgtg atgaccgttc ccttatcttt tttgaccact
     1321 tcgatgactg atatccatgc ctgaaccttc ccgtcacatt tcgaatagat agctagcctc
     1381 ggggcaatgg ccttgccatt ccactcaatc cgtgactcca gcccttccgg ttctcttccc
     1441 aaggtacaaa acaacacgcc taacattccg agcacactac cgagactcat aaatgaaatt
     1501 gtatgaaggc tttgaaactt cattgtttgg tttccaacta actaatggtt acccatatct
     1561 aataacaata atgatgccag ataatatctc attaaatttc aatcatttgt gtttttaata
     1621 gaaaattttt cccattatga aacagcaaac tgtctcaagg tgtttcagaa aaaaatgttg
     1681 aatcacgcgt gagagaaaaa ggaaggagga aacacaggaa ggaaaaaccc gttaaggacc
     1741 cgaacactca ccgtggtcgg gccctccaat tagaaccgcg acttacgcat caaatgcata
     1801 tccgagtgac aggatatatt gcgtgtccgc ttttttggtt ccaggagccg gggagttatc
     1861 gtaccgccaa ttgacctgaa acgtggtaat aaagttctcc cagatattca tacgaatacc
     1921 ctgttgagat gttacatacc aatcactggt tttatccaaa gaggggaagc cttgatgctg
     1981 atgaaacagc gtcagattgg gcaggatcgg ccagttaaga ttcaccgccc aacggccggt
     2041 aaaataattc acatcatccg cccgtttaaa gtcctcatta aaatacccca acccgacttc
     2101 accatggagt tgcaatttac ttaaatacgg gctgtcataa tctccctcat cgatgatttg
     2161 atatccaggg ccggcaaaca cggatgaacg aagattaaga tcttgaaacg tatcctgctc
     2221 aaacaacaca cctgtataca tatagaaccg cttagtgaca aagaaatcca ttttgacggt
     2281 gccataggca ttccgagcat ttaactggcc atcattctcg ccatacagat aacgtcccaa
     2341 tgtactcagt cgcaaccgtt cgcttctcgc aaccagttca cccacaaaac taaagttttt
     2401 cacatccgtg ttaccggagg taaccgaccc cccaaaatta aagttgccgg taaaggcgac
     2461 tgcctttttc gccgggggat tcaccgccaa aatcgaatca agcggcaccg ctatgggttc
     2521 aggtaacaac tcggttttga catttaacgt cccgggcttc gtcatcgccg gttttccttg
     2581 caagatggtt ccattactca agacaaaact catgggatcc tcggtatcga ttcccgtgac
     2641 ttcagaccac ttgatggtga cggggtcctt cccctcatgg aatagagtct taatctgaat
     2701 cttaccctcg gtcatttcca cgatttcccc gtacacctgg cttccatctt tcagcgtaat
     2761 ttttcccatt ggccccgcca ggcagatcac cggcagcacc atcagatata ggatcaaata
     2821 cgcgcagcac aacattagcc tcctgctcat tggacctcct tagcttggaa agacaacaag
     2881 gccaccgccg ggaaggtcgg tggatgttcc gttgacccga tccaggaaat ctgttttctc
     2941 agttggatac gaaggggggg cctggaacct gtcagattgt tcttttgtga attatcccta
     3001 ttctaacctg atttctcgct aaaaaaagtt catatgtctt atggccatgc acatgggccg
     3061 tcgattgcaa tcgatattga cgaaaccccc aacacctgta ccctgagttt aatcaccgag
     3121 agatgtccag gatttatcca gaaatgacgg aatcagcggg aggattgctc ttgagcgata
     3181 ttcaactcgg gtaaccggcg gtaaaaggta gaacggctca taccgaccaa tttaggtcaa
     3241 caacttctac gtcttaggga acactcctta agcgccatta tcctaacatt ggtaaaaaat
     3301 gggaacaagg caccaaccac agcaaatacg gagggaactt ttcatcttga gatgagtttt
     3361 ttgtaaatga gtcaaaatcc cgtttcattt tttcgctaca gtacaaggac caaacggccc
     3421 cacgtaaaca gaatgacacc tccaattatc aataaccaat ttaatcgtaa tgcataatga
     3481 attcatgcac ttagagcaac gaatcattaa tgatcgtatc aaccaggaca gagggaaaac
     3541 ctacgcgttg gaaggggtaa aacaatgaaa atccctttag aggtaacgtg agggatggaa
     3601 ccacgatata gggaaatggg aataagaagt tggggaaaga taggaagtta gattggtctt
     3661 ttcactaatc tttttcgggc tcggcgtttc catgtttatg cgtgactcga tcttcgtcaa
     3721 acagatcggc catttcttga gccattacat cattatcatg aggaagagac acaaccgaca
     3781 attctttttt tatttttttg ggagagacgg ccttgagatc tttgtcgccg gctgtttcag
     3841 aatcgttggc ttcttcggat gttttatcgt gattcatgat gacattctcc tctacgatga
     3901 ttactcaaac gattccaaat gggaccgctc aggaaacttg agctgacaac tgggacaggt
     3961 cacaaaatag accgactcac gaaccgagag gaccgctcga aaatccaggg ataccccgcg
     4021 atgtcgacag gatgggcatg gaatttcctt gagatgatag gggatatccg gctgtgtttg
     4081 tacataaaac tccatgtcgg tatggacggg aaacatatag tggcacccaa tacaaatcgc
     4141 tttccattcc ccatcgcctg tcgctgtcct ggagtcgatt ccaaaacggt tggccttgca
     4201 aataggtc
//
LOCUS       DATIAL010000032         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_113507, whole genome shotgun sequence.
ACCESSION   DATIAL010000032 DATIAL010000000
VERSION     DATIAL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113507"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VLA60_01145"
     CDS             complement(<1..617)
                     /locus_tag="VLA60_01145"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSF07986.1"
                     /translation="MRGLWASFSLAWRLLTIVPLPGRPASSIPSATFAFSLQWFPVVG
                     FLLGGSLLIIDRALEDFFPSVVLNVVILAFYALVTGGLHLDGWADTIDALAGGRDPEH
                     RLVILRDSRIGALGATGLILILGLRYAGLLTVPVGLRDGMLLCMPAIGRWAMVIGSWG
                     VVYPRSEGLAASFIRTVLWRDVLVASTVVGLGMWVMFGVVSAVVL"
     gene            complement(614..1687)
                     /gene="cobT"
                     /locus_tag="VLA60_01150"
     CDS             complement(614..1687)
                     /gene="cobT"
                     /locus_tag="VLA60_01150"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HSF07987.1"
                     /translation="MLLQNTIHDIQPVNRELYACAQAALNQLTKPVGSLGRLEDLASQ
                     YVAITQLLPPTVPAALVFTLAADHGVAEEGVSAYPSSVTAQMVENFIKGGAAINVLAR
                     QVGAKVRVVDLGVQCDLSHLPGLMVRKVGKGTKNFLMGPAMSRVEAVQSIESGIRLIM
                     DAYAEGIRLVGLGEMGIGNTTASSAITAVMTGRPVAEVTGYGTGVDAKTLTRKIQVIE
                     QGIILHCPDPLNPLDVLAKVGGFEIGGLVGMILGGAACRMPVVLDGFITAAAALLATA
                     LAPQCQAYMIPSHVSAEPGHRLVMDFLGFRPLLDLDLRLGEGTGACLGIGLLQSSLAC
                     LTQMATFKSAGVDEAVGPSPEMI"
     gene            complement(1714..>2267)
                     /gene="cobU"
                     /locus_tag="VLA60_01155"
     CDS             complement(1714..>2267)
                     /gene="cobU"
                     /locus_tag="VLA60_01155"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HSF07988.1"
                     /translation="GGSLPKPVEWNMQSRLIFVLGGARSGKSRFSLRMGRRKSPRAFV
                     ATGEPFDREMTDRIRKHKQSRGDSWTTVEVPTKVSDWLVEEGSHYSSIVVDCLTLWLN
                     NLLRDSVRPAQVPTQVKIFLKAIRACPAQVVVVSNELGLGLVPDDATSRQFRDLAGRM
                     NQLVAAEADEVYMLVSGLPLRLK"
BASE COUNT          578 a          719 c          539 g          431 t
ORIGIN      
        1 atcaggacaa cagcggaaac cacgccgaac attacccaca tccccaatcc cacgacggtg
       61 gacgccacca gaacatcccg ccataacacc gtccgaataa aggacgcggc caaaccctcc
      121 gatcgtggat agacaacgcc ccaggaaccg atcaccatcg cccatcgccc aatggcgggc
      181 atacacagca acatgccatc tcgtagcccc accggtacgg ttagaagtcc cgcataccga
      241 agccccagaa tcaatatcaa ccccgtggcc ccgagcgctc caatgcggga atcccggagg
      301 ataacgagtc gatgctcggg atcccttcct cctgcaagcg catctatcgt atccgcccat
      361 ccatccagat gaagccctcc cgtcaccaga gcatagaaag ctagaatcac cacattcaag
      421 actactgacg ggaagaagtc ttccaatgct cgatcgatga tgagaaggct ccctccaagc
      481 aaaaatccca caacaggaaa ccactggagc gagaacgcga atgtcgcgga aggaatactg
      541 gaggccggtc ttcctggtag gggcacgatg gtgagaagcc gccaggctaa cgaaaaggac
      601 gcccataggc ctctcatatc atctccgggg aaggccccac agcctcatcc accccggcac
      661 tcttgaacgt cgccatttga gtcagacatg ccagactcga ttgaagcagt ccgattccca
      721 aacaggctcc ggtgccttcc ccgagccgaa gatccaggtc cagcagaggg cgaaagccta
      781 aaaagtccat gaccaggcga tgtccgggtt ccgcagacac atgggatgga atcatatagg
      841 cttggcattg aggagccaga gcagttgcca acaaggcggc ggccgcggta ataaatccgt
      901 ccaggaccac aggcatgcga catgccgccc cacccaaaat cattcccacc aacccgccga
      961 tttcaaatcc cccgactttt gccaacacat ccaacggatt cagagggtca gggcaatgga
     1021 gaattatccc ttgctcgatc acctgaattt tccgggtcag agttttggca tccacacccg
     1081 ttccataccc cgttacttcc gccaccggtc tgccggtcat cacggcggta atcgcgctac
     1141 ttgccgtggt atttccaata cccatttctc caagcccgac cagacgaatc ccctctgcat
     1201 aggcgtccat aatcagacgg attccagatt caatgctctg gacggcttcc actcgagaca
     1261 tggccggacc catcaggaaa tttttcgttc ccttcccgac cttgcggacc atcaagccag
     1321 ggaggtggct caggtcgcat tgcacgccaa gatccaccac tcgcactttt gctccaacct
     1381 gcctggccaa gacgttaatc gcggccccac ccttgataaa attttccacc atctgggcgg
     1441 tcaccgacga aggatacgca ctcacgcctt cttcagccac tccatggtcc gccgctaacg
     1501 tgaagaccaa cgctgcagga accgttggcg gaagcaattg agtaattgcg acatactgag
     1561 aggctaaatc ctccaatcgt ccaagacttc ccacgggctt ggtcaattga ttcaaggccg
     1621 cctgagcaca ggcatacaat tcccggttga cgggttggat atcatggatg gtgttttgaa
     1681 gcagcatatt taaataccaa tgacggccat gggctatttc agccgaagag ggagaccact
     1741 cactaacata tacacctcat cagcttcggc agcgaccaac tggttcatcc ggcctgccaa
     1801 atcccgaaat tgccggctgg ttgcatcgtc gggcaccaac ccgagaccaa gttcgttact
     1861 caccaccacc acctgggcgg gacaggcacg aatggccttc aggaatattt tgacttgtgt
     1921 gggcacctgc gcagggcgca cactgtctcg caggagatta ttcagccaca gcgtcaaaca
     1981 atccacgaca atagaggaat aatgcgaacc ctcttcaacc agccaatcgg agaccttggt
     2041 cggaacttca acggtcgtcc agctatcgcc gcgcgactgt ttgtgctttc gtatccgatc
     2101 ggtcatttcc cgatcaaacg gttcacccgt ggcaacgaaa gcgcgtggag atttgcgccg
     2161 ccccatgcgt agcgaaaagc gacttttgcc ggatctggct ccacccagga caaaaatcaa
     2221 tctggattgc atgttccatt ccacgggctt gggtaaggaa ccacctc
//
LOCUS       DATIAL010000033         4093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1403878, whole genome shotgun sequence.
ACCESSION   DATIAL010000033 DATIAL010000000
VERSION     DATIAL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403878"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /gene="ppk1"
                     /locus_tag="VLA60_01160"
     CDS             complement(<1..1491)
                     /gene="ppk1"
                     /locus_tag="VLA60_01160"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HSF07989.1"
                     /translation="MTHQTKPKSPNIPEVIDNLDDPKLYLNRELSWLEFNKRVLEEAE
                     DPSHPLLERVKFLSIFFNNLDEFFMIRISGLREQLSSGVMEAALDGMSPSDQLGQIRD
                     ALLRSFSRITQCWQDDLLTRLAQAQIRILAYQDLKPKQRSLLRRYFRKEIFPALTPLA
                     FDPSHPFPHISNLTVNLAVVAHDSERGECFARIKVPSLFPRLIRIPDESQVSDDEQMG
                     LATGAESTNFVWLEDIIAANLDLLFPGLTIQASYYFRITRDADFEIEEDEAGDLLAAI
                     EEQIDLRQFGSVVRLELDRHMPDSIKDILVRNLNLAPYQVYTYDFRLGLSNLLELTAI
                     DRSDLKFPPHYPNILHDPTSKESLFSLIKREDIFLHHPYDSFSPVVDFIRQAALDPKV
                     LAIKQTLYRVGINSPVVDALMEARQNDKQVAVLLELKARFDEENNIEWARKLEDEGVH
                     VVYGVLGLKTHAKVCMVVRRESDGIRRYLHLGTGNYNPITSKFYTDF"
     gene            complement(1628..3070)
                     /locus_tag="VLA60_01165"
     CDS             complement(1628..3070)
                     /locus_tag="VLA60_01165"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HSF07990.1"
                     /translation="MPWNIHQTIEDEIKFRIPPGFQIPPDMGEPIPTRVFTSTYFDSE
                     HHRLGQLGLTLRKRVEQAHGVWQLKIPSGAVRLELKIDSGSRSIPWEFQDLLTAFFRK
                     QEAIQLGKLRTKRKGVRIHKNSRVIAEVVLDSVALIRDKKIVHSFQELEVELQEGTAA
                     QLKPIRKILLYAGAEEKAFHPKIFQALQLPYPLALELNDPSAPATEHIRKRLHSQFLQ
                     MLQHDPGTRLGRDSEALHQMRVATRRMRAIIRAVRTFLAPEWTEHVRQEMGWVGSLLG
                     EVRDGDVLLESFRHHFHDLSRQEQRAFQSILKNFEDQRSMARAKLLEGLRSDRYLTLL
                     NHFEDSLTRLPFQPSPLTITELARKAFHKVQEFVKASNSIFPKTELHRTRILLKRARY
                     ALELAEPMLGKRAKRFLEQAKHTQDLLGYHQDAVVAEQRLLAIKQHTRATGIAYVTGL
                     MVERLRNQQSQVYQQIPKQWQKLQKQGKRL"
     gene            3541..>4093
                     /locus_tag="VLA60_01170"
     CDS             3541..>4093
                     /locus_tag="VLA60_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSF07991.1"
                     /translation="MDEGLPNNQLTQLLQTAVEAAHVAAVPIRAYFEKQDLRIREKSD
                     GSPVTQADQEGEALIRTHLLSNAAIGPLDILGEEEGLQGTGTRWQWVVDPIDGTRSFI
                     HGIPLFGTIIALVDTREQFPVLGVIHLPILGLTYAAARGQGTTRQGKPLRLPEDLPIE
                     KTIIGVGDFAQFSSVNREADYHQL"
BASE COUNT          999 a          922 c         1082 g         1090 t
ORIGIN      
        1 gaaatctgta tagaatttgc tggtgatggg gttgtaattg ccggttccga gatgcagata
       61 gcgacgaatg ccatctgatt cacgacgaac gaccatgcag accttggcat gtgttttcag
      121 gcccaggaca ccatacacca catggacgcc ttcatcttct aattttcttg cccattcaat
      181 attgttttcc tcatcgaacc gggctttgag ttccaggagt acggcaacct gcttgtcatt
      241 ttgtcgggct tccatgaggg catctactac gggggagttg atgcccactc gatagagtgt
      301 ctgcttaatt gctaacactt tcggatccag agcggcttgt cgaatgaaat ccacgacggg
      361 tgagaagctg tcgtatgggt gatgaaggaa gatgtcttcc cgtttgatga gtgaaaataa
      421 tgattccttt gaggtggggt cgtgcagaat attggggtaa tgggggggga atttcagatc
      481 cgatcggtca atggcggtga gttcaaggag atttgaaagg ccgagacgaa aatcataggt
      541 gtagacttga tagggggcta aattcagatt tctgaccagg atatctttga tggaatccgg
      601 catatgccgg tccagttcca atcgcactac cgaaccgaat tggcgtaaat caatttgttc
      661 ctcaatggca gccagcaggt cgccggcttc atcttcttcg atttcaaaat cggcatccct
      721 ggtaatgcga aaataatagg aggcctgtat cgtcaggccc ggaaacaata agtccagatt
      781 ggcggcgatg atgtcctcca gccacacaaa gttggtactt tccgctccgg tggctaaccc
      841 catttgttca tcgtccgata cctgggattc gtctggaatt cggatcaatc gcgggaacaa
      901 cgacggaacc ttaatgcgtg caaaacattc tccacgctcg gagtcgtgcg ccacgacggc
      961 caggttgacc gtgaggttgg agatatgggg aaaagggtgg ctggggtcaa aggccaaggg
     1021 agtcagcgcg gggaaaatct ccttccgaaa atatcggcgg agtagggatc gttgtttggg
     1081 tttgaggtct tgataggcca gaatgcgaat ttgggcttgg gccagacgcg tcagcaggtc
     1141 atcctgccaa cattgggtga tacgggaaaa actccggaga agggcgtccc ggatttgccc
     1201 caattgatcg gatggactca tgccatccag agcagcttcc atgaccccgc tggacaattg
     1261 ttcgcgtaat ccggaaatcc ggatcatgaa aaattcatca agattattga agaatatcga
     1321 aaggaattta actcgttcca gtaatggatg gctcggatct tctgcctctt ccaggacccg
     1381 tttattgaac tccagccaac tcagttcccg gttcaggtac agcttggggt catcaagatt
     1441 atcgatgacc tcaggtatat tgggtgactt cggttttgtc tgatgagtca taatcagatc
     1501 tcctattatg cctgttatgg atgaagagtc attctacctg attgaggtta tgcaggccca
     1561 tcatcatcag tccaataaaa cgggtctatg cggacgtaaa cggtttaagg aggattggga
     1621 taaggcttta tagccttttt ccttgttttt gaagcttttg ccattgtttc gggatttgtt
     1681 gatacacctg ggattgctga tttcgtaacc gttcaaccat cagtcctgtg acataggcga
     1741 tgccggttgc ccgggtgtgt tgtttgatgg cgaggagccg ttgttcggca accaccgcat
     1801 cttgatggta tcccagaaga tcctgtgtgt gtttcgcttg ttcaagaaac cgtttggcac
     1861 gcttgccaag catgggctcg gcgagttcaa gggcatatcg cgcccgtttc aagagtattc
     1921 gagtgcgatg cagttcagtt ttcgggaaaa tgctgtttga ggccttcaca aactcctgaa
     1981 ctttgtgaaa ggcttttctt gctaattccg taatcgtgag tggagacggc tgaaatggta
     2041 aacgggttag tgagtcctca aaatgattca ggagagtgag ataccggtca ctacgcaatc
     2101 cctccagaag tttggcacgg gccatcgacc tctgatcttc aaaattcttg agaatcgatt
     2161 gaaatgcacg ctgttcttgt ctggagaggt catggaaatg atgacgaaat gattccaaaa
     2221 ggacatcccc atctctgact tcacccaaca aagaaccaac ccagcccatc tcctggcgga
     2281 catgctctgt ccattccggt gcgagaaagg tacgtaccgc tcgaatgatg gctctcatgc
     2341 ggcgggttgc gactcgcatt tgatggagcg cttcgctatc ccgacccaaa cgggtcccag
     2401 gatcatgttg caacatctga agaaattggg aatgaagcct tttccgaatg tgttctgtcg
     2461 ctggtgctga aggatcattg agttcgaggg ccagcggata gggaagttgg agtgcctgaa
     2521 aaatcttggg atggaaggct ttctcctctg ccccggcata aagcaagatc ttgcggatgg
     2581 gcttgagttg agcggctgtg ccttcctgta attcaacctc taattcctgg aatgaatgca
     2641 caattttttt gtctctgatg agggcgacgg agtccaggac gacttcagcg atgacccggc
     2701 tgtttttgtg aatgcgtacc ccttttctct ttgtccggag ttttccgagt tgaatggcct
     2761 cctgttttct aaaaaaagcg gttaataagt cctgaaattc ccagggaatg ctgcgactgc
     2821 cggaatcaat tttcaactcc agtcgaacgg caccgctggg tatctttaat tgccagaccc
     2881 catgggcttg ttctactcgt tttcggagtg ttagacccaa ttgaccgagt cgatggtgct
     2941 cgctgtcaaa ataggtggat gtgaagacgc gggtcgggat cggttccccc atgtcgggag
     3001 ggatttggaa gccgggtgga atccgaaact taatttcgtc ttcaatcgtt tggtgaatgt
     3061 tccatggcat aacaactcat cctgcactat tcattgtgcc gtgggctgtt tgaaggaaat
     3121 cttcacggga ttggtttgag ttccacaata taagcacgtc ctactttctc agaaaacctt
     3181 caataaagaa aaggaaataa tcgggctcat tttacctgaa tggatcgcaa gaaagtgtga
     3241 agctttcgcg tcacgatttg tctttacatg ataaatgtcc tgttattccc tgcacggcct
     3301 atccggtcac cttcatactc cggtgatcac gggatgcttt tcttgagcgc acttgattta
     3361 aggaaacgga tatctctttc ctgcaagctc tgttgcacgc gatgggtgat tgagtctgaa
     3421 tatgtctggt aaaaaacata ttgtgatcca gaattcgaga gaaacggtgt cgccaaaaca
     3481 ggattttcga tatactacca ggggggaaag tggggttcaa aacacgcagg agaataattg
     3541 atggacgaag gtttgcccaa taatcaactg actcaattgt tgcaaacagc tgtcgaggcg
     3601 gcccacgtgg cggctgttcc gattcgagcc tattttgaaa agcaggatct ccgtatcaga
     3661 gaaaaaagcg atggttcgcc ggtgactcaa gccgatcaag agggggaagc actcatccgc
     3721 actcacttgc tttccaacgc cgcaatcggg cctctcgata ttcttggtga agaagaaggg
     3781 ttacagggaa cgggaacaag atggcaatgg gtcgtggatc ctatcgatgg aacacgatcc
     3841 tttatccatg ggatcccgtt attcggcacc attatcgcct tggttgacac tcgggaacaa
     3901 tttcctgtcc taggagtgat ccatcttccc atactcggac tcacctatgc cgcggccaga
     3961 ggacaaggga ccacccgaca aggaaaaccc ttgcgtcttc ctgaagatct tcccattgaa
     4021 aaaacgatta tcggggtggg cgattttgct cagttttcca gtgtgaatcg ggaggcggat
     4081 tatcatcaac tgt
//
LOCUS       DATIAL010000034         4923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_973691, whole genome shotgun sequence.
ACCESSION   DATIAL010000034 DATIAL010000000
VERSION     DATIAL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973691"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2901
                     /gene="rpoB"
                     /locus_tag="VLA60_01175"
     CDS             <1..2901
                     /gene="rpoB"
                     /locus_tag="VLA60_01175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HSF07992.1"
                     /translation="LDTLEAERTNSKEEAWVEIYKRLRPGEMPSIESAKLLFENLFLN
                     PKRYDLSPVGRLKMNKRFNLDLSLEQRTLSAQDIVEVVRCLVDLKAGKGAVDDIDHLG
                     NRRVRSVGELLENQIRIGLARMERSIKERMNLLDMETVLPHDLINAKPIVGAIKEFFA
                     GSQLSQFMDQTNPLAEITHKRRLSALGPGGLTRERAGFEVRDVHPSHYSRICPIETPE
                     GPNIGLITSLATYARINEFGFIEAPFRKVRKGRVTDDVEFLSPLDGDQVVIAQANSAM
                     NKDGMLTSETITAREAGEFVTTTPDKVDYLDVSPKQVVSVATALIPFLEHDDANRALM
                     GSNMQRQAVPTIKSESPLVGTGMEAVVARDSGYLAIAQRDGIVESVDARRIVVRTGLK
                     EKDDKKKGSRILDTYDLIKFQRTNQNTCITQTPIVRIGQPVRQGQVLADGPAMDRGEL
                     ALGKNVLVAFMPWGGYNYEDAILLSEKMVKEDVFTSIHIEEFEVEARDTKLGKEEITR
                     DIPNLGEEALRNLDESGIVRIGAEVKPGDILVGKVTPKGETQLTPEEKLLRAIFGEKA
                     GDVKDTSLQVPPGIEGIVVDVKIFSRKGVDKDERSRTIETDDRVKVERDYQEELRIIE
                     EERNRKIRKLLLGQFVGRDLMDPDSGEVILKKKGRITAEVLRKLSDDDARRVILADAE
                     EQKKLDEIEREAKDQIEILQTQYDEKVGRLRRGDELPPGVIKLVKVYLAIKRKIAVGD
                     KMAGRHGNKGVVSRIMAEEDMPYLPDGTPVQIVLNPLGVPSRMNVGQILETHLGWAAK
                     ALGIQVETPVFDGASEKEIKELLKKANLPESGQSVVYDGRTGEPFKSPVTVGYMYMLK
                     LHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWALQAYGAASTLQEFLT
                     VKSDDVPGRSRMYEAIVKGENFLEPGLPESFNVLVKELQSLGLDVELIKSAD"
     gene            3012..>4923
                     /gene="rpoC"
                     /locus_tag="VLA60_01180"
     CDS             3012..>4923
                     /gene="rpoC"
                     /locus_tag="VLA60_01180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HSF07993.1"
                     /translation="MRIRIASPEKIRSWSYGEVKKPETINYRSFKPERDGLFCAKIFG
                     PTKDWECNCGKYKRMKHRGIVCDKCGVEVIQSKVRRERMGHIELAAPVAHIWFLKGVP
                     SRIGILLDMSLKQLEKILYFEAYVVLDTGNTNLKERELLTEERYRECVEEYGANSFKV
                     GIGAEAIRELLRKVDINALWDERHVKIKSTTSAAVGKKLTKRLKVIEAFHKSGNNPEW
                     MIMDAIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLVELKAPGVIIRN
                     EMRMLQEAVDALFDNGRRGRVIRGANKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGR
                     SVIVVGPELRLNQCGLPKKMALELFKPFIFHKLEERGAATTIKSAKRLVEKERPEVWD
                     VLDEVIREHPVMLNRAPTLHRLGIQAFDPILVEGKAIRLHPLVCAAFNADFDGDQMAV
                     HVPLSVEAQIEARVLMMAANNVLSPANGRPLSIPSQDMVLGCYWLTKDRDGARGEGKI
                     FSSTDEVRIAYDSQEVEEQARIKVRIDGDMIDTTVGRVILSEVLPGSMPFSHVNQLMT
                     KKELTKLIDRVYRQAGLHEVVVMLDQLKDLGFSYATKAGVSICIDNMHIPSRKKELIE
                     RAKGDVSQVQEQY"
BASE COUNT         1308 a          927 c         1401 g         1287 t
ORIGIN      
        1 ctggatacat tggaagccga gcggactaat tcaaaggaag aggcttgggt ggaaatttat
       61 aaacggcttc gacctggaga aatgccatcc attgagtcgg caaaattgtt gttcgaaaat
      121 ctctttctga atcccaagcg gtatgacctt tcccctgttg gtcggttgaa aatgaacaag
      181 cgattcaacc ttgatttgtc cttagagcag cgcacgttat ccgctcaaga tattgtcgag
      241 gtggtaagat gcctggtgga tcttaaagcc ggaaagggtg cggttgatga tatcgaccat
      301 ttaggcaatc gtcgtgtccg atcagttggt gagttattgg aaaatcaaat ccgtattggt
      361 ctggctagaa tggagcgaag cattaaggag cggatgaatc tgctcgatat ggaaaccgtg
      421 ttgcctcatg acctgatcaa tgccaagcct atcgttggtg ccatcaagga gtttttcgct
      481 ggaagtcaac tctcgcagtt tatggatcaa actaacccgt tggcagagat tacccataaa
      541 cgtaggctgt ctgctttagg gccaggtggt ttgaccaggg agcgtgcagg ttttgaggtc
      601 cgtgacgttc atccctctca ttatagtcgt atatgtccta ttgaaacgcc ggaaggaccc
      661 aatattgggt tgattacgtc gttggctacc tatgctcgga tcaatgagtt tgggtttata
      721 gaggctcctt ttcggaaagt aagaaaaggg cgcgtcacag atgacgttga attcctttcg
      781 ccattggatg gagatcaggt tgttatcgct caggctaatt cagccatgaa taaggacggg
      841 atgctcacct cggaaacgat aacggccaga gaagcagggg aatttgtgac gactactcct
      901 gataaggtgg actatctgga tgtttctcct aaacaggtgg ttagcgtggc aactgcgtta
      961 attccatttc tggagcatga tgacgcaaac cgggccctta tgggttcgaa tatgcaacgc
     1021 caggcagtgc caacaattaa aagtgagtca cctttggtcg ggacgggaat ggaagctgtt
     1081 gtagcgagag attctggata tttggctatc gctcaacgag atgggattgt tgaatcggta
     1141 gatgccagga gaattgtcgt cagaaccgga cttaaggaaa aagatgataa gaaaaaaggt
     1201 tctcgaatcc tggatactta tgatttgatc aaatttcaac gaacgaatca aaatacgtgt
     1261 atcacccaaa ctcctattgt tcgaattggg caaccggtta ggcaggggca agtgctagcg
     1321 gatggtccag ctatggatcg aggagaattg gcgttgggaa aaaatgtgct cgtcgccttt
     1381 atgccatggg gtggatacaa ctatgaggat gccattcttt taagtgagaa aatggttaaa
     1441 gaagacgtgt ttacttctat ccatatcgaa gagttcgaag tggaggcgcg tgatacaaag
     1501 ttaggaaaag aagaaatcac tcgcgatatt cctaaccttg gggaagaggc tcttcgaaat
     1561 ttggatgaaa gtggcatcgt acgaatcggt gcggaagtca agccaggcga tattcttgtg
     1621 ggaaaagtca cgcctaaggg agagactcag ctgacgccag aggaaaaatt attgcgtgcg
     1681 atttttgggg aaaaggctgg agatgtgaag gatacctcgc ttcaggtccc tccgggcatt
     1741 gaaggtattg tcgtggacgt caaaattttc tccagaaaag gcgtagataa agatgagcgg
     1801 tctcggacaa tcgagacgga tgatcgggtc aaagtcgagc gggactacca ggaagaattg
     1861 cggatcatcg aagaagaacg gaatcgtaag attcggaagc tattgcttgg gcaatttgtc
     1921 ggacgggatt taatggatcc tgatagtggg gaagtgattc taaagaaaaa gggccgtatt
     1981 accgctgaag ttcttcgcaa gttatctgat gatgatgcgc gtcgggtgat tctggctgac
     2041 gctgaagaac aaaaaaagct ggatgaaatt gaacgggaag cgaaagacca aattgaaatc
     2101 ttacagacac aatacgatga gaaggttggt cgtcttcgac gtggagatga attgcctccc
     2161 ggtgtaatta agctggtcaa ggtgtatttg gccattaaaa gaaaaattgc ggtgggcgac
     2221 aaaatggccg gccgtcatgg aaacaagggt gtcgtttctc gaattatggc tgaagaggat
     2281 atgccgtatc ttccagatgg gacgcctgtt cagattgttc tgaacccgtt gggagttccc
     2341 tctcgaatga atgtggggca aattcttgag acgcatttgg ggtgggcggc aaaagcgttg
     2401 ggaattcagg tggaaacacc cgtattcgat ggagcttcgg aaaaggaaat caaagaatta
     2461 ttgaaaaaag cgaatctccc tgagtcagga caatcggtgg tctatgatgg acggacaggc
     2521 gaaccattta agagtccggt tacggtggga tacatgtata tgctgaaact tcaccatttg
     2581 gtggacgata agattcacgc tcgctcaatc ggaccatatt ctttggtaac gcagcaaccg
     2641 cttggaggaa aggctcaatt tggcggtcag cgtctaggag agatggaggt ctgggctctg
     2701 caggcctatg gggcggcttc caccttgcag gaatttttga ccgtgaaatc agatgatgtg
     2761 cccggacggt ctcgaatgta tgaagcaatc gtgaagggtg agaactttct ggaacctggt
     2821 cttccggagt cctttaatgt cttagtgaag gaattgcaaa gtttagggtt ggatgtggag
     2881 ttgattaaat ctgctgatta gagaattggc gtggttgtcc ggtcccatca ttcaacataa
     2941 tcggaggaac gaacttggat agtatctatt cgctttttga aaaacctcgt gatgcggttt
     3001 catttgatgc catgcgaatt cgtatcgctt cgcctgagaa gattcgctcg tggtcttatg
     3061 gcgaggtaaa aaagccagag acgatcaact atcggtcctt taagcctgaa cgggatgggc
     3121 tgttttgcgc aaaaatattc gggccaacca aagattggga atgtaattgc ggtaaataca
     3181 agcggatgaa gcaccgtggg attgtgtgtg ataagtgcgg cgtggaagtc atccagtcaa
     3241 aagttcgacg cgagagaatg ggccatatcg aattggctgc tcctgttgct cacatctggt
     3301 ttctcaaggg tgtgcccagc cggattggca ttcttttgga tatgagttta aagcagttgg
     3361 agaagatcct gtattttgag gcgtacgtgg tcctggatac cggcaatacg aatttgaaag
     3421 aaagagagct cttgactgag gaaagatatc gtgagtgcgt cgaggagtat ggggccaatt
     3481 cttttaaagt gggtattggt gccgaagcta tccgggaatt attgcgcaaa gtggatatta
     3541 atgctttgtg ggatgaacgg catgtcaaga ttaagagtac gacgtccgca gcggttggga
     3601 aaaaacttac gaaacgattg aaggtcattg aggcgttcca taaatcagga aataacccag
     3661 agtggatgat tatggatgcc attccggtgt tgccccctga gcttcgccca ctggtgcctt
     3721 tggatggagg ccggttcgct acttcggatc ttaatgatct ttatcgtcgg gtgattaatc
     3781 gcaataaccg tctcaagcga ttggtggaat tgaaggcccc cggtgtcatt atccgcaatg
     3841 aaatgcgaat gctgcaggaa gcggtcgacg cgttgtttga caatgggcgt cgggggcggg
     3901 tcatccgtgg cgccaataag cgtcctctca agtctttgag cgatatgctc aaaggaaagc
     3961 aggggcgatt taggcagaat ctcctcggaa aacgggtcga ttactccggg cggtcggtga
     4021 ttgtggttgg tccagaatta cggcttaacc aatgtggatt gcccaagaaa atggctttgg
     4081 aattatttaa accgtttatc tttcataaac tggaagagcg tggagcagcc acgacaatta
     4141 aaagcgctaa gcgtttggtt gaaaaggagc gtccagaagt ctgggatgtc cttgatgaag
     4201 tgattcgaga gcatccagtg atgttgaatc gtgctccaac tcttcatcgt ctgggcattc
     4261 aggcttttga tcctattttg gtagaaggga aggctattcg tctccatccc ttagtctgtg
     4321 cggcgtttaa tgcggacttt gatggggatc aaatggctgt gcatgttccg ctttcagtgg
     4381 aagcccaaat tgaggctcgg gtattaatga tggcggccaa taatgttctt tctccggcca
     4441 atggtcgacc actttctatc ccctctcagg atatggtctt aggatgttat tggttgacca
     4501 aggatcgtga cggtgcccgt ggggagggga agatcttttc gtctacagat gaggttcgaa
     4561 ttgcctatga ttcgcaggaa gtggaagagc aggcgaggat aaaagtacgc attgatggag
     4621 atatgattga tacgacggtt ggacgagtaa ttctttctga ggttttgcca ggaagtatgc
     4681 ctttttcaca tgtaaatcag cttatgacga aaaaggagct gacgaaactg attgatcgtg
     4741 tgtatcgtca ggccggtctg catgaagtag tcgtcatgct tgatcaattg aaagatttag
     4801 ggttctccta tgccaccaag gccggcgttt ccatttgtat tgataacatg catattccgt
     4861 ctcgcaaaaa ggaattgatc gagcgcgcca aaggcgatgt ttcccaggtt caggagcaat
     4921 aca
//
LOCUS       DATIAL010000035         2830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_651914, whole genome shotgun sequence.
ACCESSION   DATIAL010000035 DATIAL010000000
VERSION     DATIAL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651914"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..701
                     /locus_tag="VLA60_01185"
     CDS             156..701
                     /locus_tag="VLA60_01185"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HSF07994.1"
                     /translation="MMSELIVVAFADETKAEEVRKLLFTMQKQYLLDLEDAVVVVKNH
                     EGKVTINQTYNLVASGATGGVLYGGLWGLLIGVLFFNPILGWAAGGLVGATAGSITGW
                     LTDIGIDDNFIKELGNTIQPGHSALFLLLKKVTPDKVINELKRKGVEGKILQTSLSTE
                     DEAKLQSFLKSKIHDEEHEVQ"
     gene            803..1393
                     /locus_tag="VLA60_01190"
     CDS             803..1393
                     /locus_tag="VLA60_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF07995.1"
                     /translation="MKRISGALILMGTMMFLLQEHSGAGSKFLALSEVCANSGIRPFT
                     QSHAFPTRTAHSGEENFLYTPTPLLSPRRMYVLGDSETLSLAEDIEQNFSVLQKLFTM
                     KVRSIETTQTPPWWQVSQYSSIALMSERMDDHERLRFVGHCGYGPTQLKIAADPVVPI
                     VHPSNPLIQRGITLTELDAIFSQTPFTGNFTNSDLE"
     gene            1914..>2830
                     /locus_tag="VLA60_01195"
     CDS             1914..>2830
                     /locus_tag="VLA60_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HSF07996.1"
                     /translation="MYVGQTLFAQLMDCLPWTTFSRIVARYGGDYRIRTLPCTEHFRI
                     LAFAQLTYRESLRDIEVCLSAQAAKLYHMGIRTPVKRATLADANERRDWRIYADFAQR
                     LIKHAHKLYATEDLGLDLANTVYALDSTTIDLCLALFPWASFRQTTAAVKLHTLLNLR
                     GAIPSFIHISNGKLHDVHALDLLLPEPGAVYVMDRGYLDFERLFTLHQAGAFFVTRAK
                     ANADMRRIYSAPNDREQGILCDQTIALSGFYTQQRYPVHLRRIRFKDLESGKGLIFLT
                     NLFGPPATTICALYKARWQVELFFKWIKQ"
BASE COUNT          708 a          714 c          691 g          717 t
ORIGIN      
        1 cttgattctg gttcagggac attcagggac acattcaact cggtggggca aggcatctgc
       61 tcgacgcctt gtcccaccag ggagatgtct tcggcagttc tgccacagat gagagcgaag
      121 ccaagctaac gataatttta acagaataag gagaaatgat gagcgaatta attgtcgttg
      181 cttttgccga tgagacaaaa gccgaagaag tacggaaact tcttttcact atgcagaaac
      241 aataccttct tgatttagag gatgcagtcg ttgtggtaaa aaatcacgaa ggaaaagtca
      301 caatcaatca gacatacaat ttggttgcct ccggggcgac aggaggagtg ctctatggag
      361 gtttatgggg tttattaatt ggggtgcttt ttttcaatcc catactaggg tgggcagcgg
      421 gaggactggt aggagcaaca gcagggagta ttaccggatg gttaacggac attggcattg
      481 acgataattt cattaaagaa ttagggaata ctatccaacc gggacattcc gctttattcc
      541 ttcttcttaa aaaagtaaca ccggataaag tgattaacga actgaagcgg aaaggcgtgg
      601 agggaaagat tttgcagact tccttatcca ccgaagacga agccaaatta caaagttttc
      661 ttaagtcaaa aattcacgat gaggagcatg aagtgcaata acaaaggatc gtcattttcc
      721 ccgtgatgac caaaggggaa cccccatttg aaaagtaacc aacagtcctg agtcgccagt
      781 aaaatctatg atacacattt ctatgaaacg aatttcgggg gcccttatcc ttatgggcac
      841 aatgatgttt ctgttacagg aacattcagg agcggggtcc aagttcctgg ctctttctga
      901 agtttgcgcc aattccggta ttcgcccgtt cactcagtca catgcatttc cgacccggac
      961 agcgcattcg ggcgaagaaa actttctcta cacacccaca cccttactct ctccgaggag
     1021 gatgtatgtt ctcggagata gtgaaactct ctctcttgct gaggatatcg aacaaaattt
     1081 ttcggtcttg caaaagttgt ttacaatgaa ggtacgaagt attgagacga cccaaacacc
     1141 tccgtggtgg caggtctctc aatactcatc aattgcccta atgagtgaac gaatggacga
     1201 ccatgagcgc ctgcggttcg tgggccattg cgggtacggg cccacccaat tgaagattgc
     1261 cgctgatccg gtcgtaccaa ttgttcatcc ctcaaacccg cttattcaaa ggggaataac
     1321 tctaactgaa ttagatgcaa ttttttctca aacacctttc acgggaaact tcaccaattc
     1381 agacctggaa tgatctaggt ctcgaaggaa attggccatc tcggcaaata attctccatg
     1441 gttatgatgc atcagtcagc ttgtatggct ttttcaaaaa tcatgccctt cagggaaagg
     1501 accttcataa tcgcgttctt cgacatcctg gttccggtca ggtcgttttc gctgtggggt
     1561 aagagaaagg agcacttggt tttacgaaac tcagcgcagt tacgcctcaa gtaggaattg
     1621 ttcctttacg ggtaatttcc caacaattgc ctattgacaa taattccagg ttgaacccgg
     1681 cgtatcctct tacacattac ctgtacgggt atctgaagct aagtccgcag acacatcctg
     1741 acgaggcggc aattgaatta ttaaagttcc tcactactgt ccggttaact gagtcgcctt
     1801 tcggcctaag cgcatgaatg aattacatgt tttggcaaat ttggatggtg tcgacttgaa
     1861 cggcgacccg ccttcaggtg attgtccccc gggtaacccc cggaggattc acgatgtatg
     1921 tcggccaaac cctgtttgcg caactgatgg attgcttgcc ttggaccacg ttttcccgaa
     1981 tcgtcgcccg ctatggtggc gattatcgta ttcggacgct gccctgcacc gaacactttc
     2041 ggatcttggc ctttgctcag ttgacctatc gcgaaagcct gcgcgatatc gaggtctgtc
     2101 tgtcggcgca agccgccaag ctgtatcaca tgggaattcg caccccggtg aaacgggcca
     2161 cgctggccga tgccaacgag cgccgagact ggcggatcta tgccgacttc gcccagcgct
     2221 tgatcaagca tgcgcacaaa ctgtacgcca cagaagatct gggcctggac cttgcgaaca
     2281 cggtgtacgc gctggactcc acgaccatcg atctgtgtct ggccctgttt ccgtgggcct
     2341 cgtttcgcca gaccacggcc gcggtcaaac tgcacaccct gttgaacctt cgtggggcga
     2401 ttcccagttt tatccatatt tccaatggga aactgcacga tgtccacgca ctcgacctgc
     2461 tgcttccaga gcctggagcg gtgtacgtca tggatcgcgg gtacttggac ttcgaacggc
     2521 tgttcacgct gcatcaggcc ggcgccttct ttgtcacccg tgccaaggcg aatgccgaca
     2581 tgcgccgcat ctattcggcc cccaacgatc gggagcaggg cattctgtgc gatcagacca
     2641 tcgcgttgtc cggattctac acgcaacaga ggtatcctgt tcatctgcgt cgtattcggt
     2701 tcaaagatct cgagtcgggc aaaggcctca tcttcctgac caacctcttt ggtccgccag
     2761 caaccaccat ctgtgccttg tacaaggctc gctggcaagt cgaattgttt ttcaaatgga
     2821 tcaagcagca
//
LOCUS       DATIAL010000036         8750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_329893, whole genome shotgun sequence.
ACCESSION   DATIAL010000036 DATIAL010000000
VERSION     DATIAL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329893"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..3793)
                     /locus_tag="VLA60_01200"
     CDS             complement(335..3793)
                     /locus_tag="VLA60_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HSF07997.1"
                     /translation="MTESSSASPPPAAQSAALRGLLVAQFFGAFNDNAWKLIVALLAI
                     KQVAASVGASGPDFEAASQTTTTQAFVIFTLPLMVVSAFAGVFSDRFSKRTVIVVMKA
                     VEVLLMGAGTAALFWNPLGGVLPLLVLAAMGAQSAIFSPSKYGILPELLPHHRLSWGN
                     GQLEMWTFIAIIAGTALAGPLLDLSDQSPWLTGLVLLVCSGIGLWASLFIPTVPRARL
                     EGGVQETWKAAIEAHRSDRVLKLGIMGAMAFWTLASLVGQDVLIYAKAVLQLSDSLSG
                     LPLAAFGVGIGIGSLLVGKLSAAKVELGYLPLGGTGITASLFALGFGEPQLGGTLMAM
                     GGLGLASGFVVVPLNALIQWRSPADRRGAVIAFANTLVFGGVLLGSLGSGFLSKIGLS
                     ASNIFLVSGIGSATLTIWALRVLPEMFIRLMLILFTHTIYRLTITGRDRIPQEGGALL
                     VPNHVSFIDGLLLLATTDRPIRFLVDQHYYDHRLLHPFAKIMGVIPISSNGSPREILH
                     ALRQAGQSLDRGELVCIFPEGQITRTGNLLPFRSGFTRIVKGRDVPIIPINLDRVWGS
                     IFSFIGGRFLAKWPTRFPYPITLSIGKPLPSTTSAEEVRHAIQELGEAAWRLRKPTRR
                     PLHHSFVWSMRKHPLRVVFGDATRPRVSCFQALTGAIALGRALRPRWEGQHTVGILLP
                     PSVGGALANVAATLSGRTTVNLNYTVGVQGLESAATQAGLVTVLTSRVFLVKAKLELP
                     INVTPIWIEEIRNTINLQARLTAALLALFAPIRMLERLSGATIHPSIDDIATIIFSSG
                     STGEPKGVLLSHFNLDSNVEGIAQVLHLDQNDRVLGILPFFHSFGYLATLWFPVIHGA
                     SVIYHPSPLDAGPIGDLIHQHRITILLATPTFLQLYLRRCTPEQFGSLRIVLTGAEKL
                     PDRLLVAFEERFGIRPIEGYGVTECAPVIALNCPDFRAAGFFQTASRRGTVGQPLPGV
                     SVRIVDPDTEQPLPVGTPGMLLVKGPNVMDGYLGREDLTAKVIRQGWYITGDIAALDE
                     DGFITITDRLSRFSKIGGEMVPHGRVEKALLEAANAETMVLAVTAVPDERKGEQLVVL
                     HTLEESAIPEILEKVSASGLPNLFIPKRDNFIKVDHLPVLGTGKLDLRTLKRLALEKL
                     SKAQLP"
     gene            4625..5443
                     /locus_tag="VLA60_01205"
     CDS             4625..5443
                     /locus_tag="VLA60_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF07998.1"
                     /translation="MGAAGESIFHRMRNVLNSIHVSAGLINHRLREFHVADVGRVADM
                     LEAHSHEYGRYLTQDPKGKRIPHFLGQLSEVLAQNHLDTLTELTTLNYHLEQLEYFLT
                     VGQAPLRVGGFKDTIQFAKIMDEALAPHQGELDRMGVQVCREYQMVGEGILEVSKLLR
                     IVVNLIRNAINGMRKITDRTHRLTLSVLPCPDREGFVRLQVADTGSGIPLDCLTRVFS
                     PQAPGEQSDLLPNLHASAIAAKELAGSLRVWSDGPMQGAIFTLDLPVIHMEGER"
     gene            5443..6006
                     /locus_tag="VLA60_01210"
     CDS             5443..6006
                     /locus_tag="VLA60_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF07999.1"
                     /translation="MEKEQGNRRILVIDDNVAIHDDFRKILERSLDTSLLQEVRAELF
                     DDVEEQKIVEKFEVETADQGQMGHRMVQDAMKAERPYAVAFVDMRMPPGWDGVETVEH
                     LWEEDPDLQIVICTAFSDHAWEDVIQRLNNNGQLLILRKPFDNIEVWQLANSLTKRWA
                     ESRQAKSQLELLAKWAEERAEETIKAH"
     gene            6289..7575
                     /locus_tag="VLA60_01215"
     CDS             6289..7575
                     /locus_tag="VLA60_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08000.1"
                     /translation="MSEFNRQVLSEFPILRVGIVGLQERDLSPSFQRVLASQGSMLQI
                     TWLDYASLHLSCFDSDNPFDLILVDCRVKGKGEEIACHIKKTCDNAFILGLMHDDISD
                     AEGINEPAIDWLVNEHMAGIAMPWAIQEGLVRRRGLIEREQLRRQVEDASYNIEMGEI
                     ASTVLHNVGNVLNSVNVAVHVVHELIHQSSVILVHRIAELLRRHDENWEIFLTQDPKG
                     KRIPPAIMKLGRHLLEEQQTVLKELEGLVRNIDHVKQIIISHQTMAKSRGTSEALSVV
                     EILDQAVELSFQPGDAKWIRIQRDYQPVPAVVTDRHQLLQILVNLLRNAKQAMQVQDG
                     VDHQLTIRVEVRTDDVFSVVITIQDTGMGIAPEHLARMFTRGFTTKRDGNGIGLHSSM
                     AKIHRMGGLLQAHSEGVGAGATLTLILPVEAGAGPT"
     gene            7572..>8750
                     /locus_tag="VLA60_01220"
     CDS             7572..>8750
                     /locus_tag="VLA60_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HSF08001.1"
                     /translation="MNPVCTHNRRILVIDDSPSVLQNFRKILCPAIRFISDAWWEGSH
                     DLFDEPFLHHGKEPFDLNCVDNGESGRDLVTEAKAEGSPFAVVFLDVQLSNGWDGFET
                     VKRIWDEDSDVQVVFCTAYPDFGWSEVLPGLGRRDQLLILRKPFDPIEVWQLSTVLTM
                     KWHWAQQARLRVRDLEEVVMKRTGLLEETNRRLEEALLRGQAVEVQLAQATRDVEERT
                     LELSAVRDHALNEIHERERVEVILRHKTDQLARSNRDLEQFASVAAHDLQEPLHSIQV
                     FLDLLRLKYGSALNTHGLGYVDRVKNAADRMQQLIQSLLVYSRVEFPQMAEEKLVLRE
                     LVEEILSDLGARIEESRAVVHLGEFPTIYGNAIQIRQLLQNLLGNALKFHQPGVPPVI
                     R"
BASE COUNT         2196 a         1959 c         2404 g         2191 t
ORIGIN      
        1 ggcataaaga agggggatat cgattagaaa aggaagtatt ggaagtattt ccagtaaaca
       61 ggcgaagcgg tccttgggtt tgacggccta ccaaagtaga agaagcacta atcccattat
      121 ccaaaaacct cacatttaga tttaggcata gttcctcagg atacggaaaa cgccttcggt
      181 ccgtgtagtc ggaagattgt gccgcaataa actctatttc attgttattt gcgggtactg
      241 ttcaggtact tcttggtcag tcattttttt gcctctgtgt gcccatgagt tagccgaaga
      301 tgaagtcagt tgggggcagg ttcattgact ggtattaagg aagttgtgcc tttgataatt
      361 tttccagtgc cagtcgtttt aatgtgcgca agtccaactt tcccgtcccc agtacaggga
      421 gatgatctac tttgataaag ttgtcccgtt tgggaatgaa cagattgggc aatccactgg
      481 ccgagacctt ttccaagatt tcaggaatgg ccgattcttc gagggtgtgt aagaccacca
      541 gctgctctcc ttttcgttca tcgggcacag cggtgacagc caaaaccatg gtttcggcgt
      601 ttgcggcttc caataaggcc ttttcaactc gtccgtgagg aaccatctcg ccaccgattt
      661 tggaaaaccg agataggcga tcggtaatcg tgataaatcc atcctcatcg agtgcggcaa
      721 tatctccggt aatgtaccat ccctgtcgta ttaccttggc ggttaaatct tcacggccta
      781 aatacccatc cattacgttg ggtcccttga ctaacagcat gccaggagtg ccgacgggca
      841 atggttgttc ggtatcagga tcgacaattc tgacggacac tccgggaagg ggctgaccga
      901 ccgttccccg ccgggaggcg gtttggaaaa accccgccgc ccgaaaatct ggacagttaa
      961 gagcaatcac cggcgcacat tctgtgaccc cgtatccctc aatgggtcga atcccaaatc
     1021 gttcttcgaa tgcgaccaga agccggtctg gtaatttttc cgcgcctgtc aggacgatcc
     1081 gcagggaacc gaattgttct ggggtgcagc ggcgaaggta caattgtagg aatgtcgggg
     1141 ttgctaacag gatggtaatt cgatgctggt ggatcaggtc tccaatgggc ccggcatcca
     1201 atggagaagg atgatagatc acgctcgctc catggataac cggaaaccac agtgtggcca
     1261 gatatccaaa agaatgaaaa aacggcaaaa tacctaaaac tcggtcgttc tggtccaggt
     1321 gcaagacctg ggcaatgcct tccacgtttg aatcaaggtt gaaatgactc agcaggacgc
     1381 ctttgggttc tcccgtgctc ccgctactaa aaatgattgt ggcgatatca tcgatagaag
     1441 gatgtattgt ggctccagag agacgctcca acatccggat tggagcgaag agcgctaaca
     1501 acgccgcggt gagccgagcc tgcaaattaa tcgtatttcg aatctcctcg atccagatgg
     1561 gagtgacgtt aatcggcaat tccaatttgg cttttactaa aaagacccga ctggtgagaa
     1621 cagtcacgag cccagcttgt gtggcggcag attccaaccc ttgcacgccg accgtgtaat
     1681 tcaaattgac ggtggttctt ccagaaagag ttgcggccac attggcaagc gctccaccta
     1741 cacttggcgg cagcagaatt cccaccgtat gttgcccttc ccaacgaggt ctgagtgctc
     1801 ttcccagggc aatggcgcct gttagcgcct ggaagcagga cacacgaggt cgggttgcat
     1861 caccaaatac aacacgaagc gggtgcttgc gcatggacca tacaaaacta tgatggagag
     1921 ggcgccgggt gggttttcgt aaacgccacg cagcttctcc taattcctga atggcgtgcc
     1981 gtacctcttc ggcagatgtg gttgagggga ggggtttgcc aatagagagc gtaatgggat
     2041 aagggaagcg agtaggccac tttgctaaaa aacgtccgcc gatgaaactg aagatactcc
     2101 cccagacccg gtcaaggttg atgggaataa tggggacatc ccggcctttg acgatgcggg
     2161 tgaatcccga ccggaagggt agtaggttac cggtacgggt aatttggcct tctggaaaaa
     2221 tgcagaccag ttcgcctcga tcgagacttt gtcctgcttg ccgtaacgca tgtaaaattt
     2281 ctctgggaga tccgttcgag gaaattggaa tgacccccat gattttggca aagggatgga
     2341 gcaggcgatg gtcatagtaa tgttggtcga cgagaaatcg aatagggcga tcggtggtgg
     2401 cgagtaggag cagtccgtca atgaacgaga catggttggg taccagaagt gccccgcctt
     2461 cttgcggaat acgatcccgc ccggtgatgg tgagtcgata aatggtgtga gtgaacaaga
     2521 ttaacatcaa ccggataaac atttcgggga gaacccgaag cgcccaaatg gttagagtcg
     2581 cgctcccgat tcctgatacg agaaagatat tgctggcgct aagcccgatc tttgagagga
     2641 atcctgatcc cagagatccc aacaggactc ctccaaagac cagggtattg gcaaaggcaa
     2701 tcacggcgcc tcgtcgatca gctggggatc gccattgaat gagggcgttg agcgggacca
     2761 ccacaaatcc gctggcgagg cccagacccc ccatggccat gagggttcct cctaactgtg
     2821 gctccccaaa ccctaaggcg aaaagactgg cggttatccc ggtgcctccc aagggaaggt
     2881 agcctaattc aactttggcc gctgagagtt ttccgaccaa cagggagcca atgcctattc
     2941 cgactccaaa ggctgccagg ggtagccctg aaagagaatc agataattga agaacggctt
     3001 ttgcgtagat caacacatct tgcccaacca aactggccaa cgtccaaaat gccatggccc
     3061 ccatgatgcc caatttcaag acccgatctg accgatgggc ttcaatggcg gccttccagg
     3121 tttcttgaac tccgccctca agtcgagccc gtgggactgt gggaataaac aatgaagccc
     3181 ataatcctat tcccgagcac accaaaagca ctaatcctgt tagccatggg gattgatcgg
     3241 aaaggtccag caagggaccc gccaatgcgg tccctgcaat gatggcgatg aaggtccaca
     3301 tctcgagttg accatttccc catgacaggc gatggtgagg taggagttca ggaagaatcc
     3361 catatttgga aggactaaaa atggcacttt gagcacccat tgcggctagt accaggaggg
     3421 ggaggactcc accaagcgga ttccaaaaaa gggcagcagt cccggctccc atcaacaaga
     3481 cttctacggc tttcatgacg acgatgacgg ttcgcttact gaaccgatcg gaaaagactc
     3541 cggcaaaggc cgaaaccacc atcaggggaa gggtaaagat gacaaaggct tgcgtggtgg
     3601 tggtctggga ggctgcttca aaatccggcc cagaggcacc tactgaggcg gcgacttgct
     3661 taatagccag gagggcaaca atgagtttcc aggcattatc attaaaggct ccaaaaaatt
     3721 gcgcgacgag cagtccgcgg agagctgcgg attgagctgc gggtggtggg gaggctgagg
     3781 aagattcagt cacggggatt acgaggcgtg agtatgggtt ttttcttctt ttatccttga
     3841 attcaaggag atattaaaca aatacatatc ataacgaaat tgaaactatc tgttggttgt
     3901 tccctgaatt ggactcgaaa ggctgaagaa tctgggtgtc ccctgagacc gagaaggttg
     3961 aatggtagca tggtttgtat tatttccaag taaaaacaag tcgagacggc tttgagaatt
     4021 caaaaggggt taagtcattt tttatatagc gagataatta agttgagtta tcttgtcgaa
     4081 gccattcttt cccgtcaatc attttttggt ccatgttttt gattctttgt tgtgaattgg
     4141 tgaaatcatg gttcgaacct taagggagga tcatcttatc aaagaaatca caccagtacc
     4201 tccttgcagt ttcgatcgat acctcttgtg aagtgatgct tccgttttaa agattcggaa
     4261 attctccacc gttctgggag tgcatgccgt attctagata ccgcacgcgc cttttcttcc
     4321 aaggttaaac tgtatctctt gagaatccgg cttttctcag gatgcaggaa catgcgagtt
     4381 aagcttccaa ggctcttaag gttccttcgt atacagccga ttcattccca tatgccgcgc
     4441 caaggatatg gttgagatgt tttggcatgt tctcttccgt tttcttaacc gggattcgtg
     4501 caaacgcttt agaatcaata gatttcacaa tattaagttg aaacgccatc tcggtaaagg
     4561 gacctcgacg gctattttcg cgatgacgac tcacacgaaa tcatcagggg tttcggaagg
     4621 accaatggga gcagcggggg agtccatctt ccatcgaatg cggaacgtct tgaatagcat
     4681 acatgtgtct gccgggctca ttaaccatcg gcttcgggaa tttcatgtgg cagatgtagg
     4741 gcgggttgca gatatgttgg aggcccatag ccatgaatat gggaggtacc tcactcagga
     4801 tcccaaaggg aaaaggattc cacatttctt ggggcagtta agtgaggtat tggcccagaa
     4861 ccacctcgac actctgacag aattgaccac actcaattat caccttgaac aattggaata
     4921 ttttctgacg gttgggcaag caccgcttcg ggtcggagga tttaaagata ccattcagtt
     4981 tgcaaagatt atggacgagg ccttggctcc tcatcagggt gaattggatc ggatgggggt
     5041 ccaagtgtgt cgggagtatc aaatggttgg cgaaggtatt ctggaggtat ccaagctcct
     5101 ccgcattgtg gtgaatttga tccggaacgc gatcaatggg atgcggaaga taaccgaccg
     5161 gacccatcgc ctcactctat ctgtgttgcc ctgcccggac cgcgaagggt ttgtccgctt
     5221 gcaggtcgct gatactggaa gcggaatccc tttggattgt ctcacacggg tgttttcccc
     5281 gcaggctccg ggggagcaat cagacctgtt gccgaatctt catgccagtg ctatagcggc
     5341 caaagagctt gctggatcgc tgcgggtgtg gagtgacggt ccaatgcagg gagcaatatt
     5401 tacccttgat ttgccagtta ttcacatgga gggagaacgg taatggagaa ggaacaaggc
     5461 aaccggcgta ttttggtgat tgatgataat gtggcaatcc acgatgattt tcgaaaaatt
     5521 cttgagcgat cattagatac ctcgctgctc caagaggttc gagccgaatt gtttgatgat
     5581 gtggaagaac aaaaaattgt tgaaaagttt gaagttgaaa ctgcggacca agggcaaatg
     5641 ggacatcgga tggtgcaaga tgccatgaag gctgaacggc cgtatgcggt tgcctttgtg
     5701 gatatgcgca tgcctccagg ttgggatggc gtagagactg tggagcatct gtgggaagaa
     5761 gatcctgatt tgcagattgt gatttgtacg gcattctcgg accatgcatg ggaagatgtc
     5821 attcaacgat tgaataacaa tggccaactc ctcatcttgc gaaagccctt tgataatatt
     5881 gaggtttggc agttggcgaa ttcccttacc aaacgttggg cggaatcgcg tcaagcaaag
     5941 tcgcagttgg aattattagc caaatgggca gaagaacggg ccgaggaaac aatcaaagct
     6001 cattgaagta aggacatctc tcatattctg tagatttgtg cgtaggtgat aatctctttt
     6061 atggaaggat atttgtggat taggggatgc ctttctttac agagtgcgca atacatcggc
     6121 aagtcaggga agcgttaatt ctcatgttcc tgagaatgaa aaaccatgga tgttcaaggc
     6181 gaaatttcag tcgtccaccg tggactgctg cttttgtatg ttgatgagct atttgtacct
     6241 tcatgattga tcttctctcc ttatcccctt catgtgatgg tccacggcat gtcggaattt
     6301 aatcggcagg tgctttcaga atttccgatt cttcgtgtgg ggatagtggg acttcaggaa
     6361 agggatcttt ccccatcctt tcaaagggtg ttggcttctc agggttccat gcttcagatc
     6421 acttggcttg actatgcctc cttgcatctt tcctgttttg actccgacaa cccatttgat
     6481 ctcattcttg tggactgtcg cgtaaagggg aagggggagg agattgcttg ccatatcaaa
     6541 aaaacctgtg acaatgcgtt cattttggga ttgatgcatg atgatatttc cgatgcggag
     6601 ggaataaacg aaccggctat tgattggctg gttaatgaac atatggcggg tatcgcgatg
     6661 ccctgggcca ttcaagaggg gttggttcga cgtcggggct tgatcgaacg ggagcaattg
     6721 cgcaggcagg tggaagatgc ctcttataat attgagatgg gggaaatcgc ctcaaccgtg
     6781 ttgcacaatg tgggtaatgt gctcaatagc gtcaatgtgg cggtgcatgt ggttcatgag
     6841 ttgattcatc agtcttcggt gattttggtt catcgcattg cggagctgtt aaggcggcat
     6901 gatgagaatt gggaaatctt cctgactcag gatccaaaag gaaaacggat tccaccggcg
     6961 atcatgaaat tggggaggca cctcctagaa gagcaacaga ccgtgctaaa agaattggaa
     7021 ggcctggttc gcaatattga tcacgtgaaa caaattatca tttcacatca gaccatggcg
     7081 aagtctcggg gcaccagtga ggctctctct gtggtggaaa ttctggacca agcggtggag
     7141 ttaagttttc aacctgggga tgcgaagtgg atcaggatcc aacgagacta tcagcctgtc
     7201 ccggctgtgg taacggatcg gcatcagctc ctacaaattc tcgtgaatct gctacgaaat
     7261 gctaaacagg cgatgcaggt acaggacgga gttgaccacc agctcaccat tcgagtcgaa
     7321 gttcggacag atgatgtatt ttccgttgtc atcacaattc aagacacggg catgggaatc
     7381 gcgcctgaac atttggccag aatgtttact cgcggtttta cgaccaaacg cgatggcaat
     7441 gggattggtc tgcatagttc gatggccaag atccatcgta tgggtggatt gttgcaggcc
     7501 catagcgaag gggttggcgc cggagcaacc ttgacactga tccttccggt ggaggcaggg
     7561 gcaggaccga catgaatccc gtctgtaccc ataatcgacg gatcttagtg atcgatgata
     7621 gcccgagcgt tttacagaat tttcggaaaa ttctgtgtcc cgccataagg ttcatttccg
     7681 atgcgtggtg ggaaggcagt catgatcttt ttgatgagcc cttcctgcat catggaaaag
     7741 aacccttcga tctcaattgt gtggacaatg gggagtcagg acgtgatctg gtcacggaag
     7801 ctaaggcgga gggttctccc tttgcggtgg tgttcttgga tgtgcaattg tccaatggct
     7861 gggacgggtt tgaaacggtt aagcgaattt gggatgagga ttctgacgta caggtcgtat
     7921 tttgtacggc ctatcctgat tttggttggt cagaggtatt gcccggatta ggacgccggg
     7981 atcaattatt aattttacgt aagccctttg atccgattga ggtgtggcaa ttgtctacgg
     8041 ttttgacgat gaagtggcat tgggctcagc aggcccggtt gcgggttcgg gatctcgaag
     8101 aggtcgtcat gaagcgtacg ggcctccttg aagaaactaa ccgtcgtttg gaggaggcat
     8161 tgctcagggg acaagcggtt gaagtgcagt tggctcaagc gacacgtgat gttgaagaac
     8221 gcactctcga actttctgct gttcgggacc atgcgttgaa tgaaatccat gaacgggaac
     8281 gggtcgaagt catattgcgt cacaaaaccg atcaattggc caggtcgaat cgggacttgg
     8341 agcaatttgc gtcggttgca gcccatgatt tacaggaacc gcttcattcc atacaggtgt
     8401 ttcttgatct cttacggctg aagtatggat cagcccttaa cacccatggt ctagggtatg
     8461 ttgatcgcgt caaaaatgcg gctgatcgta tgcaacagct gattcaaagc ctgttggtgt
     8521 attccagggt cgagtttccc caaatggcag aggagaagct tgtcttgcgc gagttggttg
     8581 aggaaatcct atcggatctc ggcgcccgga tcgaggaatc gcgggctgtc gtccatcttg
     8641 gggaattccc caccatttat ggaaatgcga ttcaaatcag gcaattgtta caaaatttgc
     8701 tgggcaatgc cttgaaattt catcagccgg gggtgccacc tgtcattcgt
//
LOCUS       DATIAL010000037         3508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_544617, whole genome shotgun sequence.
ACCESSION   DATIAL010000037 DATIAL010000000
VERSION     DATIAL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544617"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..851)
                     /locus_tag="VLA60_01225"
     CDS             complement(234..851)
                     /locus_tag="VLA60_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HSF08002.1"
                     /translation="MFSFMPRESGFFDLFEQASQNVVEGGQCLKALMKSFDEPHTQIQ
                     HIKNLEHKGDDLTRDIVYKLNKTFITPLDREDIHALASALDDILDEIDAVAELFMVFK
                     IDHPTPTALRLSEILHDAVLEVGKGIDLLRHQNWDMKDCAIRVHSLENEADRVSREAI
                     SRLFEEEADPKTVMKWKEIYENFEMGTDSCEDVVNVLERIALKHG"
     gene            complement(1137..2696)
                     /locus_tag="VLA60_01230"
     CDS             complement(1137..2696)
                     /locus_tag="VLA60_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HSF08003.1"
                     /translation="MKRLAVIDIGTNSIHMVLAEIGKGFAYKIVDRIKEMARLGDGTF
                     TSQRLSPDAMDRGLTVLKRFSMLAKNKGFDPILPIATSAVREAKNGGDFLKLVRKELG
                     LRVRVITGEEEARLIYLGVRNSMDLAHFPAMIVDIGGGSVELMACTQTRLKFVRSLKL
                     GAIRLKDQFLKADIPDKKMIQRLENLVGQTLKKSLSSKKRDSQFNQLVATSGMAGNLA
                     EIIYLARTGRPLSQIDMATVELDEVREVERLLRTKDTQSRLKIPGLDPRRVDTLYSGV
                     LVLRTLMERIGVKQARISDKAIREGVIYDFIQQHQEGLRAEQEIPHVRRRQVLLLARR
                     YQYPKNHAHHVAKLALSLFDQTQALHQLGDREREWLEYAALLHDIGHHISENKHHKHT
                     YYLITHADLPGFSSEEVDIMANVARYHRRGQPTIRHKGFRLLSREQRKVVPCLSAILR
                     IADGLDRSHFSVVRKISIEPGKPLRLHVFFRYDPELELWTTERRMKLFEKIFHCRIEL
                     KPAPIAVKTVA"
     gene            complement(2796..3107)
                     /locus_tag="VLA60_01235"
     CDS             complement(2796..3107)
                     /locus_tag="VLA60_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HSF08004.1"
                     /translation="MRDLVLQPQTFRVVFRAQAVFLTRLEFRLLEQLLDSEGRSLYRQ
                     DLLDRGWGPQVPVEVLTEDSHIVRLRRKLQRVGDHGPTIETVWGVGYRIRIIDDYSVP
                     S"
BASE COUNT          941 a          920 c          769 g          878 t
ORIGIN      
        1 cataaaggcc ccgatcacat tcaagacccc ggcaaaaaga acagccaagg cgggactcag
       61 gacccgagtg gacacgacgg tggccacggc attggcacta tcatgccaac catttgaaaa
      121 atcaaacaga agtgcgagag cgataattaa aataagaagt atgctgattt cggccacgga
      181 ggtgctatgg gaaggggtat cagaaaaatc ggaaggatcc ggctaccact tcattatcca
      241 tgcttgagtg cgatccgttc caggacattc accacatcct cacacgaatc ggtgcccatc
      301 tcaaaattct catagatctc tttccacttc atcaccgtct tgggatccgc ttcctcttca
      361 aataaccggg agatcgcttc ccggctcacc cggtcagctt cgttttccaa gctatggact
      421 cgaatggcac agtccttcat atcccagttt tgatgacgaa gaaggtcaat ccctttcccg
      481 acttctaaaa cggcatcatg aaggatttcg gacaacctga gcgccgtggg cgtcgggtgg
      541 tcaattttaa acaccatgaa caattcggcc accgcatcaa tttcgtccag aatatcatcg
      601 agcgcgctgg ccaatgcgtg aatatcttcc cgatctaacg gggtaataaa tgttttattg
      661 agtttataca caatgtcacg agtgagatcg tcacccttat gctccaaatt tttgatgtgt
      721 tgaatttgtg tatgcggttc atcaaaggac ttcattagtg ctttcaagca ctgccctcct
      781 tccacgacgt tttgagatgc ttgctcgaat aaatcaaaaa acccgctttc ccgaggcata
      841 aaactaaaca taaggagtct ccttaaactg gaaagcgcag aatggtcaaa tcccgtcttt
      901 ggactctgca ggccagggat cacaatttcc aagccgaacc caggttagcc taccgattat
      961 tcgttttttt tggtgagcag taattatgga gtgacccgcc atgatccaca aggagaagtc
     1021 gacacactca aaccgcagtt ctacatttta acctatgcta aggactccaa atttggcaag
     1081 tatcccacaa atgcccgaac aatttccaga gaaggattca gacataggat gccccgctag
     1141 gcgacagttt tcactgcaat gggagccggc ttcaattcaa tacgacaatg gaagattttc
     1201 tcaaagagtt tcatccttcg ttccgttgtc cataattcca actccggatc atagcgaaaa
     1261 aatacatgga ggcgcagagg cttcccgggt tcaatggaaa ttttcctgac cacggaaaaa
     1321 tgactccgat ccaggccatc ggcaattcgt aaaattgcac tgagacatgg cacaactttt
     1381 cgctgctcgc gacttaagag tcgaaatcct ttatgcctga tcgtgggttg gccgcgtcga
     1441 tgatatcgcg ccacgttggc cataatatct acttcttcag acgaaaaccc gggaagatcg
     1501 gcatgggtga tcaaataata ggtgtgtttg tggtgtttgt tctcactaat atgatgccca
     1561 atatcgtgaa gtaaagccgc atattccaac cactcccgct ccctgtcgcc aagttgatgc
     1621 aatgcttggg tctgatcgaa caaacttaat gccaacttcg ccacgtgatg ggcatggttt
     1681 tttggatatt ggtatcgtct ggccaacaat aagacctgtc ggcgtcgaac atgaggaatc
     1741 tcctgttccg cccgcaaccc ttcctgatgc tgttggatga aatcgtaaat aaccccctcc
     1801 cgaatcgctt tatcactgat gcgcgcttgc ttgactccga tacgttccat cagtgtacgc
     1861 aacaccaaca caccggaata cagggtatcc acccgcctgg ggtccaggcc ggggatcttc
     1921 aatcgcgact gtgtatcttt tgtccgaagc agtcgttcaa cctctcgaac ttcatctaac
     1981 tcgaccgtgg ccatatcaat ttgtgagagc ggccgtccgg tccgagccaa ataaatgatt
     2041 tccgcgaggt ttcctgccat ccccgatgtg gctacaagtt gattaaattg agagtcacgt
     2101 ttctttgatg ataaggattt cttcaaggtt tgaccgacca gattttccag acgttggatc
     2161 atttttttat ccggaatatc tgccttgagg aattgatcct tcaatctaat cgcacctaat
     2221 ttgaggcttc tcacaaattt caagcgcgtt tgcgtacaag ccatcaattc cacggatccg
     2281 cccccgatat ctacaatcat ggcgggaaaa tgggcgaggt ccatactatt cctgaccccc
     2341 aaatagatga ggcgggcctc ttcctcaccg gtgatgaccc gcactctaag cccgagttcc
     2401 ttgcgaacga gctttaaaaa atcccctcca ttttttgctt cccggaccgc actggtggca
     2461 atcggtaaaa tcggatcaaa gcccttattc tttgccagca tggaaaaccg ttttaggaca
     2521 gtcaacccac ggtccatggc gtccggagac agccgctgtg acgtaaaggt tccatcaccc
     2581 aacctggcca tttccttaat acggtccaca atcttgtaag cgaatccttt tccaatttcg
     2641 gctagaacca tgtgaatgga attagttcca atatcaataa ccgcaagcct tttcatgggg
     2701 ctgaactcct tcaaaaatag attcggttct acccgttcat gcccacagag attccgtggc
     2761 caataccggt ataaggtttt gtacgaagga agccttcagg agggaacaga ataatcatca
     2821 atgatacgaa tacgataccc cacaccccag acagtttcaa tagtagggcc gtgatccccc
     2881 acacgctgca gttttctccg gaggcggacg atatgagaat cttcggtcag aacttcgaca
     2941 gggacctgcg gtccccagcc gcggtccaag agatcctgcc gatacagaga tcggccctcg
     3001 ctatcaagca attgttccaa taaccgaaat tcaagtcgtg tgagaaagac cgcttgtgct
     3061 cgaaagacga cccggaaggt ttgtggttgg aggacaagat ccctcataca ctcacatgac
     3121 aaactcggat catctgctca ttctctcatg aacaaatctg aatcacccaa aatttataca
     3181 cgactggtat ccggccttat ggaaacggta tctgacagac atttcctccc cgttatagga
     3241 atgtgacaaa cgggtaaatc tccgctgatt gcctagggag cattcgaaat actggaggcc
     3301 ttcatatgtt aggcgcccga tacggcactt cggaagtgct ttacgaagaa ctacatctac
     3361 cggcaaaatc ccgaaaattt caaaaggaga gttctcgcgc gagatgttga acgagcaaac
     3421 ggttcccaaa tagggcatca acctttccgg ataccttcga gggacgaagg attgccaccc
     3481 ctccggcctc ttcaattaac acttgagt
//
LOCUS       DATIAL010000038         6497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1082348, whole genome shotgun sequence.
ACCESSION   DATIAL010000038 DATIAL010000000
VERSION     DATIAL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082348"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..411
                     /locus_tag="VLA60_01240"
     CDS             247..411
                     /locus_tag="VLA60_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08005.1"
                     /translation="MAHLRRIQQALFRPLPVRQAAERLSTTVTRNNSAPLTTHPISDA
                     GTSIVYLTAV"
     gene            complement(428..1036)
                     /locus_tag="VLA60_01245"
     CDS             complement(428..1036)
                     /locus_tag="VLA60_01245"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HSF08006.1"
                     /translation="MNGRLGCLPIFLFLLLLFLLPLVFAQFMGLALVKLRLDPSVAML
                     VIVGIFLGSVVNIPITRIARQDEVIAHPYPLFGFMNIGSHLQRSPRETILAVNLGGCV
                     VPTLSAGEAGRGDRYRHAGIGALAGGSADSATPCPRRSAIGGLMSPGCSVPSWALISC
                     TYGRSRASPPGWPASGEPAPLTALSSRESWPHIWRNPMKKLV"
     gene            complement(1207..1353)
                     /locus_tag="VLA60_01250"
     CDS             complement(1207..1353)
                     /locus_tag="VLA60_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HSF08007.1"
                     /translation="MIFREGGYLALPDLHGAKRILTAAALTYVASSLFSLVNIWRWIR
                     LVWR"
     gene            1545..1778
                     /locus_tag="VLA60_01255"
     CDS             1545..1778
                     /locus_tag="VLA60_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HSF08008.1"
                     /translation="MAQVLVRQLDEKIVDRLKKRAQEHGRSLESEVRTILEEAVPDYE
                     GAWKRIQQFHKRLKKSGRTFCDSAELIREDRDR"
     gene            1787..2206
                     /locus_tag="VLA60_01260"
     CDS             1787..2206
                     /locus_tag="VLA60_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSF08009.1"
                     /translation="MIDANIAIKWFIPEIYSDVALHASRLQAPLHVPAFIQLEAGSVL
                     AKKIRRNELTKEEGDVILKEFRQLPLQYHPDERLFHAGYALALATHQSLYDCLYLALA
                     ETIDGAVITADRKFFQALSTGPYGTRIIWIEDIVRLE"
     gene            complement(2347..3030)
                     /locus_tag="VLA60_01265"
     CDS             complement(2347..3030)
                     /locus_tag="VLA60_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HSF08010.1"
                     /translation="MRMILLFLLLMIVVLGPQLWVWWVFRQYRHHREDLGGTGGELAR
                     HLLNRFGLQQVRVEPTPMGDHYNPETKVVGLNPQHYNGRSLTAAVIAAHEVGHALQDH
                     EGYTLLKDRTHLIKFAQKAEKAGSYLMLGIPVLAGVTRVPAVGLAVLVVAIGTMSVSA
                     MVHLITLPVEWNASFRRALPILREGGYLAPPDLHGAKRILTAAALTYVASSLFSLVNM
                     WRWIRLVRR"
     gene            complement(3150..3938)
                     /locus_tag="VLA60_01270"
     CDS             complement(3150..3938)
                     /locus_tag="VLA60_01270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF08011.1"
                     /translation="MNLELNGKTALVTASSGGIGLEIARALAAEGAKVVINGRTQASV
                     EQAMAKVRADLPHGEMIPLVADNGTAAGCSLTISQVPEVDILVNNLGIYEAIGFFEET
                     DQAWQEMFEINIMSGVRLARHYLHGMLECGHGRVVFISSESGVSPAPEMAHYSATKTM
                     QLSIARSLAELTKGTEVTVNSVLPGPTRTESVETFIRNIFPDLPPAEAGRRFMSENRP
                     TSLIGRLIDPKEIGEIVAFVCSARASVINGSCIRAEGGLVRTVF"
     gene            complement(4013..5482)
                     /locus_tag="VLA60_01275"
     CDS             complement(4013..5482)
                     /locus_tag="VLA60_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HSF08012.1"
                     /translation="MAKLEQLNFQNTYAGLPDVFHERVKPTPFPNPYLVSVNPAAAEL
                     LDIDPTEWTRPEFAEYFCGARLLPGSDPIAMLYSGHQFGHYVPQLGDGRAIMLGEVRN
                     QNGERWELQLKGAGLTRFSRDGDGRAVMRSTIREYLCGEAMHGLGIPTTRSLCIVAGE
                     EIVWRETPEPGAMLLRMAPTHVRFGSFEVFYYRRQHEYLKTLADYVIEHHYPHLVGAE
                     NPYARLLHEVAVRTGQLVAQWQAVGWAHGVLNTDNMSILGLTLDYGPFGFMERYDPTF
                     ICNHSDHHGRYSFQNQPDIGYWNIRALARALSPLVGQDEVNGIPEIYEKAMLAKYAEL
                     MRDKLGLVEAHAGDDKLVTDLLNLMDSSRVDYTNLFRGLGAVRQESSSAPDELRDQFL
                     HREAFDDWTARYRERLRAEKSVDEERHVRMDQVNPKYILRNHLAQQAISQAVQQKDYS
                     EVDRLLNLLGDPFTDRPGMESYAAPPPPGEPPIIVSCSS"
     gene            complement(6245..6433)
                     /locus_tag="VLA60_01280"
     CDS             complement(6245..6433)
                     /locus_tag="VLA60_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08013.1"
                     /translation="MHLLELFIGHDPLSMAIALVLVLSVLAYGWFEVRRTARLPHIGM
                     VLIKNRLKTTSKFPTPRR"
BASE COUNT         1593 a         1730 c         1661 g         1513 t
ORIGIN      
        1 catgtagagg gaaatttcag ttatcagtct atgcctgagc ctaccataaa gaaaaaaaat
       61 gatcgaagca tcctgcaatt cgaatatagt tttaggtgta ggaactggaa ttcttagcgg
      121 cgtttcaatt tggatcttta gatttgcggt agattcagca attgtatgga tcggaaaatt
      181 atcttttctt tcaaattttc tgaatatgga aagtgaatta gagagcaact ttttcacgag
      241 gatgcgttgg cacatcttcg ccggattcag caggccctgt tcaggccgct tcccgtccgt
      301 caggccgctg aacgcttgag cacgactgtc accaggaaca actcagcccc attgacgacc
      361 caccccatca gcgacgccgg aaccagtatt gtgtacctca ctgctgtgta gccaaatcca
      421 catcacatca caccagtttt ttcatcgggt tacgccagat atgcggccag gattcccgag
      481 aggacaatgc cgtcaaaggt gccggctccc ccgatgctgg ccatcccggt ggcgatgcgc
      541 gggatcgccc gtatgtgcaa gatatcagcg cccaggaggg gaccgaacac cccggcgaca
      601 taaggccacc gatggcgctt cgtcgggggc aaggagtagc gctgtcagcg ctgccaccag
      661 cgagggcacc aatgccggca tggcgatacc gatcccctcg accggcttcg ccagccgata
      721 gcgtgggaac gacgcagcct cccagattca cggccaggat ggtttcccgc gggcttcgtt
      781 gaaggtgcga cccaatgttc ataaagccaa acagggggta ggggtgagcg atcacttcat
      841 cctgacgggc aatacgtgta atgggaatat tgacgacact ccccaggaaa attcccacaa
      901 tcacgagcat agcgacagag gggtccaatc gtagtttgac caaggccagg cccatgaatt
      961 gggcaaacac gagtggaagc aggaagagca gcagcagaaa caggaaaatc ggaaggcaac
     1021 ctaatcgtcc gttcatggga gaaattccag tcctgtccgg ccgttaattt gaaaaatggt
     1081 gctcagcaca ttcagggaac ttggcacatg catcagcaaa caataagacc tgacgtcgga
     1141 aagaagactg cccattttcc gatccgcttg tcggtccagg atttatcgag cttgttcccc
     1201 aaacacttaa cgccaaacca atcgaatcca tcgccagata ttcaccaggc tgaagagtga
     1261 ggaggcgaca taggtgagag cggcggcggt gaggatacgt ttggcgccat gcaaatccgg
     1321 aagggccaaa tagccgcctt ctcggaagat catattccgc tcgtcgatta ttcccccacc
     1381 tttagtaact agctggcatc ttcttctcat accgttcttt tttcaccaac gatacaaaga
     1441 aaccagtatt ccatatgacc gatacgttga agaaatagct ctattgatgg atgccagatc
     1501 acatgctatc ataatgatag cattttttaa tattggagga gacgatggca caggttttag
     1561 ttcgacaact tgacgagaaa atcgtagatc ggctcaagaa acgtgctcag gaacatgggc
     1621 ggtcccttga atcggaggtc agaacgattt tggaagaagc cgttcctgat tatgagggag
     1681 catggaaacg gatccaacag tttcacaagc ggttaaagaa gtccggccgg acgttctgcg
     1741 atagtgccga gctgatccgg gaggatcgag atcgttgaag ggctacgtga ttgatgcgaa
     1801 tatcgcgatc aagtggttta ttcctgaaat ttattcggat gttgcgctgc atgccagccg
     1861 gttacaggct ccacttcacg tgccggcttt tatccaattg gaggcgggca gcgttcttgc
     1921 caaaaagatc cgccgcaacg aactcacgaa agaggaaggt gatgtcatac tcaaggagtt
     1981 caggcaactc ccgcttcaat accatcccga tgaacgactc tttcacgcag gctatgctct
     2041 ggcccttgcg actcatcaaa gtttgtacga ttgtctctac cttgctttgg ccgagacaat
     2101 tgatggtgct gtgatcacgg ccgatcgcaa attcttccag gcactttcca ctgggcccta
     2161 tggcactaga atcatttgga ttgaagatat cgttcgattg gaatagcggt gagaaaacgg
     2221 cgcattgcac ctacccaaac ctcatacccg gagcgcgctg cattcaggag aaaaatatta
     2281 ttcttctccc cctcctttta caacccatat tccaaagatg aaatggcgct ttgcgccgga
     2341 gaaaaactag cgccggacca atcgaatcca tcgccacatg ttcaccaggc tgaagagaga
     2401 ggaggcgaca taggtaaggg cggcggcggt gaggatacgc ttggcgccat gcaaatccgg
     2461 aggtgccaga tagccgccct ctctgaggat cggcagggct cgacggaaac tggcattcca
     2521 ttccacgggt agggtgatga ggtgcaccat ggcagagacg ctcatggttc caatcgccac
     2581 gactaagacg gcaagaccca cggcaggaac ccgggtcact ccagccagga ccggaatacc
     2641 caacatcaga tacgatccgg ccttttcggc tttctgcgca aatttgatca ggtgagttcg
     2701 atctttcaag agggtatagc cctcatgatc ttgcaaagca tggccgactt catgcgctgc
     2761 aattactgcc gcagtgaggg atctgccgtt gtaatgctgc gggttcaaac caacgacttt
     2821 ggtttcggga ttgtaatggt ctcccattgg cgtgggttcc acacgcacct gttgcagacc
     2881 aaaacgattg agcaaatgcc gcgctaattc cccgcccgtt cctccaagat cctcccggtg
     2941 atggcgatat tggcgaaaga cccaccacac ccacagttgg ggtcccaaca caacaatcat
     3001 gagcaggaga aaaagaagaa tcatgcgcat ggaattactc tatctcctca gcccgctcct
     3061 taccaagaaa gtgattgctc cttctattgt tttcctgatt acactcaagg atttttcgaa
     3121 gaggtctttt tgcttattgg cctgtttcat caaaagaccg tacgcacgag acccccttcg
     3181 gcccggatgc aggacccatt gatcaccgag gcgcgggcgc tacagacgaa cgccacgatc
     3241 tctcctattt cttttggatc aatcagccga ccgatcaggg aggtcggacg attctccgac
     3301 ataaaacggc gtccggcttc cgcgggcgga agatcaggaa atatgttccg gatgaacgtc
     3361 tccacgcttt ccgtgcgggt gggaccgggg agcactgaat tgaccgtgac ctccgtccct
     3421 ttggtgagtt cggccaatga gcgtgcgatg cttaattgca tggttttggt tgcgctgtag
     3481 tgcgccattt cgggagccgg agagacgccc gattcgcttg agatgaagac gacacgcccg
     3541 tgaccacatt caagcatgcc gtggaggtaa tgacgggcga gccgcacgcc gctcatgata
     3601 ttgatttcga acatctcctg ccacgcctga tccgtttcct cgaaaaatcc gatagcctcg
     3661 tagatgccga gattgttaac cagaatatcg acctcgggca cttgggagat ggtgagactg
     3721 cacccggcgg cagttccgtt gtcagcaaca agaggaatca tttctccatg gggaagatcg
     3781 gcccggacct tcgccatagc ttgttccacg ctggcctgcg tacgaccgtt aatcacgact
     3841 ttagcgccct cggcggcgag agcacgcgcg atttccagcc ctatgcctcc tgaggacgcc
     3901 gtcaccaagg cggttttacc gttcagttcc agattcatga tgacacttcc ttcccatgac
     3961 ggctgattgg aaatccccga acaggttttt acctatattg ttgttgaatt gttcacgaag
     4021 agcacgaaac gataatcggt ggctcaccgg gaggcggagg cgcagcatag gattccatgc
     4081 cggggcggtc agtaaacggg tcgcccagca gattcaataa tcgatcaact tcggaatagt
     4141 ccttctgctg aaccgcttgg ctaatggcct gttgtgcgag atgatttcga aggatgtact
     4201 tgggattcac ctgatccatt ctgacgtggc gctcttcatc aacgcttttt tcggcccgta
     4261 gccgttcccg atatcgtgcg gtccaatcgt caaacgcttc ccgatgaagg aattggtccc
     4321 gtaattcgtc cggcgctgac gaactctcct gacgaacggc tcccaatcca cggaacaagt
     4381 tggtgtaatc cacccggctg gagtccatga ggttcaacag atcggtcacg agcttatcgt
     4441 ctcccgcgtg cgcttcgacc agccccagct tgtcccgcat caattcggca tacttcgcca
     4501 gcatcgcttt ttcatagatt tccggaatgc cgttgacttc gtcttggccc actaacggtg
     4561 acaaggctct ggccagcgca cggatattcc aataaccgat gtcgggttga ttctgaaagg
     4621 aataccggcc gtgatggtct gaatgattac agataaaagt cggatcgtag cgttccataa
     4681 atccgaacgg accgtaatcc agcgtcaacc ccaggatcga catgttatcg gtgttcagga
     4741 caccatgcgc ccaccccaca gcttgccatt gagccacaag ctgcccggtg cgaaccgcga
     4801 cctcatgcaa cagcctggca tagggattct ctgctccgac caaatgcgga taatgatgtt
     4861 cgatgacata atctgccagc gttttcaggt attcatgttg gcgacgatag taaaacacct
     4921 caaaactgcc aaaccgcaca tgggtcggcg ccatccggag cagcatggct cccggttccg
     4981 gcgtttcccg ccagacaatt tcttctccgg ccacaataca taaactgcgg gttgtgggaa
     5041 tgcccaatcc atgcatggct tcgccacaga gatattcccg aatggtcgaa cgcatcactg
     5101 cacgaccatc gccatcgcgt gagaatcgcg tcaggcctgc gcctttcaat tgcaactccc
     5161 accgctcccc gttttgattg cgcacctccc ccagcatgat ggcccgccca tcgccgagct
     5221 gcggcacgta atgcccgaat tgatgccccg aatacaacat ggcaatggga tcgctcccag
     5281 gcaacagtct ggccccgcag aaatattcgg caaactccgg acgggtccat tccgtgggat
     5341 caatatcgag taattcggca gctgccgggt tcacgctcac aaggtaagga ttaggaaaag
     5401 gcgttggctt gacccgctca tgaaacacat caggaaggcc cgcataggtg ttctgaaagt
     5461 tgagttgttc taatttggcc atgacttcat tatccgtgac ctagccgtgg tcgggcaacc
     5521 acaaacattt ttgcatcact ttagagacct ttgaaatttc aacggtgccc gcaaaaggct
     5581 tcaagacact cggcaagaaa tgccacatca gcatgtgctt ccaaaggctt tcctcagaac
     5641 cgtatctctt tcgattcaac tgtgtgtggt attttctcaa caataattct tttgattgca
     5701 ttggaaattg aaaaacatga atatttacaa tacattacga ataaaatttt tctaggttca
     5761 ataattgctt ttgccataat gcagaagtct tagtgctggg aagcttatgg ttattgggtg
     5821 tgcgtattgt ttcgtgtgcc ctcgaaaaaa ggagatcgca tgattcggat ttgtgcatgg
     5881 tgtcgacagg aaggcaaggc tggaattctt ggaaggaacc ctgtggagtc tggtgaaatc
     5941 aatgaaccgg aatcccacgg gatctgccac gagcatggac tccgccttcg ccaagccttc
     6001 aggcgaaacc ttggccatcg ccctctttca accgccacac tctcgccctc ttcagcctcc
     6061 ttctagacag cgttcaccct tgcctgatgc tgaaattcct gttgcaggat ggtagatggc
     6121 agaagtgtat gccccgaccg gagggtcgat atacatcacg gctgcgcaca ggggaatcgc
     6181 aagagctagg gtttgtcggg gttgcgtctt gaggccggca atcgtaaaga ctgggaaagg
     6241 tttactatcg gcgcggagtt gggaatttgc tggtcgtctt taatcggttt ttgatcagga
     6301 ccatcccgat atgagggagg cgtgcggttc gacggacctc aaaccatcca tatgccagca
     6361 cgctcaggac caacacaagc gctatggcca tcgaaagagg gtcatgcccg ataaataatt
     6421 ctaagagatg caccgtggac cattctcctg atgtattctc atatttttcg gtcctttccg
     6481 cccgatctta agatttt
//
LOCUS       DATIAL010000039         4211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_330227, whole genome shotgun sequence.
ACCESSION   DATIAL010000039 DATIAL010000000
VERSION     DATIAL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330227"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1954)
                     /locus_tag="VLA60_01285"
     CDS             complement(<1..1954)
                     /locus_tag="VLA60_01285"
                     /inference="COORDINATES: protein motif:HMM:NF020928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HSF08014.1"
                     /translation="MNSALHDQIIAKELKISDKQVAATVSLLDDGATVPFLSRYRKEV
                     TGGLDEVMITSIRDRIAQLRELDKRRDVILSSLEEQGKLTDVLQGQVHAATTMTELED
                     IYLPFRPKRRTRAMIAKERGLEPLALSLWAQEAQLHVEAEAQKFLNPELSVETVEDAL
                     AGARDIMAEWISEDLQARASMRALYLEQGIFKTTAVRGKDVQGSKYRDYVEWEEPVAK
                     APSHRVLAMRRGETEGFLTFRVMVPEPEALSLLHRLFLKGKGPASEQVILAIKDSFTR
                     LLSLSMETEARLVTKTRADQTAIEVFAQNVQQLLMAPPLGQKTVLAIDPGFRTGCKMV
                     CLDRQGTLRHTETIFPHLGASGAAKAGDTVVELCQRFQVEAIAVGNGTAGRETETFLR
                     ALKLPNAIPIVMVNESGASVYSASRVAREEFPDQDVTVRGAVSIGRRLMDPLAELVKI
                     DPKAIGVGQYQHDVDQGILKQRLQDVVISCVNRVGVDVNMASPQLLTAVSGVGPQLAT
                     NIVAYRQEHGPFATRTALKKVPRLGAKAFEQAAGFLRITDGEHPLDASAVHPERYAVV
                     NAMAKDLGCTVKDLMKDPARQRRIDLNRYITEDVGKPTLMDILTELAKPGRDPRQQFE
                     AVKFDEGVQSIEQVTPGMILSGVVTNV"
     gene            complement(2050..2340)
                     /locus_tag="VLA60_01290"
     CDS             complement(2050..2340)
                     /locus_tag="VLA60_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08015.1"
                     /translation="MMRAWLVVCVGSLFLYGCTEVKYIKAGATEADFEADRVECRSQI
                     LMSPPGPVIPSGQMGKPGVSQGFNTKSAEQSAQQQVDQCLRAKGWEPEPQPK"
     gene            2452..3162
                     /locus_tag="VLA60_01295"
     CDS             2452..3162
                     /locus_tag="VLA60_01295"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSF08016.1"
                     /translation="MNYTFPQLQRLSVLLCVIVSLSGCAQKIEPLNFSGFLTDYTSLR
                     PSPDDSGAWSYRKPGVNFKGYDHIILDPLVIWPSQHSEYGGLDALTAWKLALGFQDSM
                     SRALAGGYVIVKEPGPGVLRLRAALTDVLLERPSVSTPDQILPLANDLLIQASEKISG
                     INALEGEAAIEVEILDTQSRERLVAYVEKRMSSEILLTRDKDSLGPVLEIFDYWGKKL
                     RQRLDEERGLREYRKDIQ"
     gene            complement(3630..>4211)
                     /locus_tag="VLA60_01300"
     CDS             complement(3630..>4211)
                     /locus_tag="VLA60_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSF08017.1"
                     /translation="SNLGPIGTALQNNGLLNKKLDVGITVPQSLMFSAYHDLSRQWAI
                     MGDFGWQDWSEFGKVDIGVDTANPTNLIANLNYQDTWHVAFGTQYRVNPAWVVLTGFA
                     YDSSMVKDKHRTLSVPVGETYKFGFGALWQKTPTLNLGFSYELAWGGDLPVDQNRGPL
                     SGRVSGDFKNTTFHFFALTVTWGEGVKMGPGGA"
BASE COUNT          909 a         1219 c         1090 g          993 t
ORIGIN      
        1 tgacattggt caccactccg gacaggatca tgccgggtgt gacctgctcg atggattgca
       61 ccccttcatc aaactttacg gcttcaaatt gttggcgcgg atcacggccg ggcttggcca
      121 attcggtgag aatatccatt aaggtcggtt tgcccacgtc ctccgtgatg tatcgattga
      181 gatcgatcct gcgttgccgg gcaggatcct tcatgagatc cttcacggtg cagcctaagt
      241 ctttggccat cgcgttgacc acggcatacc gttcgggatg cacggcgctg gcatccagcg
      301 gatgctcgcc atcggtgatg cgtaaaaatc cggcagcttg ctcaaacgct ttggctccca
      361 gacgcgggac tttctttaag gcggttcggg tggcaaacgg gccatgctcc tgtcgatagg
      421 ccacaatatt cgtggcaagt tgcggaccca caccggagac ggccgtgagt agttgcgggc
      481 ttgccatatt cacatccacc cctacccgat tgacacaact gatgaccaca tcctgcaacc
      541 gttgtttcaa gatgccctga tcgacatcat gttggtattg cccgacgccg atggccttgg
      601 gatcgatctt caccagttcg gctaagggat ccatgagccg tcgtccgatc gagacagcgc
      661 cacgcaccgt cacatcctga tcgggaaatt cctcacgtgc gacccgggac gcggaataga
      721 cggacgcacc gctctcgttg accatcacaa tggggatggc gtttggtaat ttcaatgcgc
      781 ggagaaacgt ttcggtctcc ctcccggctg tcccattgcc caccgcgatc gcctcaacct
      841 gaaaacgctg acacaactcc accaccgtgt ctcccgcttt ggcggccccg cttgctccca
      901 gatgcggaaa gatggtctcc gtatgacgca aggtgccctg gcgatccagg cagaccattt
      961 tacatccggt gcgaaagccc ggatcgatgg ccagcacggt cttctgtccc agcggtgggg
     1021 ccatgagcag ttgctgtaca ttttgcgcaa acacttctat cgccgtttga tcggcgcggg
     1081 ttttggtgac caggcgggct tcggtttcca ttgagagtga caggagcctg gtaaaactgt
     1141 ccttgatcgc caggatgacc tgttccgatg ccggcccttt acctttcaga aaaaggcgat
     1201 gcaaaagcga cagcgcttcg ggttccggca ccatcacccg aaaggtcaga aacccttctg
     1261 tttctccacg ccgcatggcc agcacccgat gtgagggcgc cttggcgacc ggttcttccc
     1321 attcgacata atcccgatac ttgctgcctt gaacatcttt cccccgcacg gcagtggttt
     1381 tgaaaatccc ctgctccaga tacagcgccc gcatggaggc gcgggcctgc agatcttcgc
     1441 tgatccattc ggccatgata tcgcgcgcgc cggccaacgc gtcctccacg gtctccacac
     1501 tgagttccgg gttaagaaat ttttgtgcct cggcttcgac atgcaattgg gcctcttgcg
     1561 cccagaggct caacgccaac ggttccaatc cccgctcttt ggcgatcatg gccctcgtgc
     1621 ggcgtttggg gcggaagggt aaatagatgt cttccaattc ggtcatggtc gtggccgcgt
     1681 gcacctgccc ttgcagcacg tcggtgagtt tgccttgttc ctcgagcgag gacaggatga
     1741 cgtcccgtcg cttatccaat tcccgaagct gcgcgatacg atcccgaatg gaggtgatca
     1801 tgacctcatc caacccgccc gtcacttctt tgcgataccg cgatagaaac ggcaccgtgg
     1861 cgccatcatc cagcaagctc accgtggctg cgacctgctt gtcggagatc ttcagttcct
     1921 tcgcaatgat ctgatcatgc aatgctgaat tcatattttc gttccatcct tactgaatca
     1981 tgagtgaaat tttcgtcaag agtcgttctt cacaacgaca gcccgattgc ccttacatca
     2041 ctcggcaact cacttgggtt gaggttccgg ttcccatcct tttgctcgca aacactggtc
     2101 aacttgttgt tgtgccgact gttcggccga tttcgtgttg aacccttggc tgacccctgg
     2161 ttttcccatt tgaccactgg ggatgaccgg gccgggtggc gacataagga tttgactgcg
     2221 gcactcgact ctatcagcct caaaatccgc ttccgtggct ccagccttga tatacttcac
     2281 ttcagtgcat ccatagagga aaagacttcc aacacaaacc acaagccatg ctctcatcat
     2341 tggacaccct ctttttcaaa tggaggtact ttgaagtgcg gccgtaatat ggcctttatg
     2401 aaggagacgg gatatactcg tcaactttcg atctcaaaaa atgaggatac cgtgaattac
     2461 acctttccgc aattacaacg actgagtgtg ctcttgtgcg tgatcgtcag cctctccggc
     2521 tgtgcacaaa aaatcgagcc gttgaatttt tcaggatttt taaccgatta caccagcctt
     2581 cgtccctcgc cggatgacag cggtgcctgg agttatcgga agccgggtgt caatttcaaa
     2641 ggatacgacc acattattct ggatcccctg gtgatctggc ccagccaaca ttctgaatat
     2701 ggcggccttg atgccttgac cgcctggaaa ttggctctgg gattccagga cagtatgagc
     2761 cgggcgttgg cgggaggcta tgtgattgtg aaagagcccg gtccgggggt cctgcggctt
     2821 cgagcggccc tgacggacgt gttgttggaa cgcccgtcag tgtccacgcc ggaccagatc
     2881 cttcctctgg ccaatgatct cctgatccag gcttcggaaa aaatttccgg cataaatgcc
     2941 ctggagggag aggcggccat tgaggtcgaa atattagata ctcagagccg ggaacgactg
     3001 gtggcgtatg tggaaaagcg gatgagctca gagatcctcc tgacccgcga caaagattcc
     3061 ctggggccgg tgctggagat ctttgactat tggggcaaga aacttcgaca acggttagac
     3121 gaggaacgcg ggctacggga gtatcggaaa gatattcaat aatccgtggt ggtgttctta
     3181 agatagcagt gcatccgcat acggaattgg accagccggc catgcgtgcg tcatcccatc
     3241 cggaattcat acgaatgggc cgggccgttc actcatccat cgcgatggcc ggctaacgaa
     3301 agcccctgac tccccgttgc tgatagagcg cagcaatttc cgaggttttc tccatcctcc
     3361 ccttctcacg ttgatccgac acctgcgggc gaatgttgaa aattttcata cggaaatttc
     3421 aaccccgtga cgatggagat ggccggttca cacgatggtt cacgtgatca atccacgtac
     3481 ccttcacccg atacccaagc gaccagtacg aacattcatt ctctcaggct gtcatgatcg
     3541 aagtgcaaat tggaggaatc aatcctgagt ccatcaacga accattcccc gcgaccttgt
     3601 cactgatacg ctccaaaacg acggttgcgt tatgctcctc ccggtcccat cttcacccct
     3661 tctccccagg ttacggtcaa tgcaaaaaag tggaaggtgg tgttcttaaa gtcaccggac
     3721 acccggcctg acagcggtcc acgattttga tcaaccggca aatctccccc ccaggccagt
     3781 tcataggaaa aaccaagatt gagggtgggc gtcttttgcc aaagggctcc aaacccgaac
     3841 ttataggttt caccaacggg cacgctgaga gtgcgatgtt tatccttcac catggaacta
     3901 tcataggcaa aacccgttaa aaccacccat gcggggttca cgcggtattg ggtcccaaac
     3961 gccacatgcc aggtatcctg atagttcagg ttggcgatga ggtttgtggg gttcgccgta
     4021 tccacgccga tatccacctt cccgaactca gaccagtcct gccatccaaa gtcacccatg
     4081 atcgcccatt gcctgctgag gtcgtggtag gcgctgaaca tcagcgattg agggaccgtg
     4141 atccccacat cgagtttctt gttcagcaaa ccgttattct gcagggcggt accgatagga
     4201 ccaagattcg a
//
LOCUS       DATIAL010000040         3326 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1513005, whole genome shotgun sequence.
ACCESSION   DATIAL010000040 DATIAL010000000
VERSION     DATIAL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513005"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VLA60_01305"
     CDS             <1..956
                     /locus_tag="VLA60_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08018.1"
                     /translation="SIDCLVPKRFQKEHSQHRAGFLHDPTARAMGVGRDLFACRKDGS
                     EFPVEIGLNPINTPEGFMVLSSVVDITARKRSEAELERLNQQLRMQNQELEAYAYAVS
                     HDLRAPLRAVHNYADFLLEDLSSKVSIEEKEYLTGITTAVCEAEELVSDLLELSRLNY
                     HDSNPQPCYIGNLIHKVLRTLNFGDDVHVQLPTEWPTILGEEHLLKQIFQNLISNGVK
                     FNRSSQKHIEVDWRPERKRFVRFFIRDNGIGILPQYQKQIFQIFQRLHTTRDYEGTGI
                     GLAIAKKAVTKLGGEIRVESETGKGSIFSFTVPMGEMHDDE"
     gene            943..1404
                     /locus_tag="VLA60_01310"
     CDS             943..1404
                     /locus_tag="VLA60_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08019.1"
                     /translation="MMMNESRQITILMAEDNAHDVLATKRAWHQFGFGNHLYIVSDGE
                     ECLDYLYHRGKYQDSNIYLTPDLILLDLKLPKLDGHQVLQSIRADKKFRFLPVIILTN
                     STLETDRNKGYEYGCTAFLKKPVGYSNFANLIKSIRTFWSCVEPYAQPLMC"
     gene            1420..>3326
                     /locus_tag="VLA60_01315"
     CDS             1420..>3326
                     /locus_tag="VLA60_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08020.1"
                     /translation="MSIVHPSGLSALLIEDDTHERAAIRRALRNDIQTLLECETAEEA
                     IRRLDQSPSEFDVIISDLKLPGMDGLTFFRTLRRMKCSSPFILLTANGSEQTAIEAFK
                     AGVDDYLIKATQSGYLEMLSPLIAQVVHHHRMKIRNELLELSLREANERFQQFMEQYQ
                     GIFWLTDYEEPRKVLYVSPTYKIIWDRSAHQLNQYSYDWMEALHPDDRIRVRTAYLEL
                     DATHKYNETYRIIKPDGSLRWIHDRGFLITDPSGKPYRIARLTEDITDRTTMSEALQA
                     SEERFAKFMAHLPGLAFIKDEHCRFIYLNPAFEKVFNITIADWIGKTVHDVLPPETAA
                     RLHTNDKWVLSHRQSLATVEKIPLPDGFHQYLTVKFPILRNDRSPFLGGVSTDVTERL
                     QAETQRDQIFSLSPDLMCIIGMDGYLKKINPAFEKLFGYTEEEFLSQPLIVFTHPDDR
                     PSTLAAFHQLSEEITVFDFHNRLRDIRGNYRWLEWRATAQIQEQAIYAVARDITQRYH
                     ATEALRRYEQIVSTSSDFLCFLDKDDCFQAVNRRFSEFFNLSVRDIIGRHLRDFVGQD
                     IFDSLKFHLEDCRKGQAVRFQHKLKFPDGNLRFMEARLNPFLEHDGSIAGVTATWRDL
                     TEQKYLEQHMT"
BASE COUNT          883 a          847 c          749 g          847 t
ORIGIN      
        1 aatccattga ttgcctggtt ccaaaacgtt ttcaaaagga acattcccaa cacagagccg
       61 gatttcttca cgacccgacg gccagagcga tgggagtcgg tcgggatctc ttcgcctgcc
      121 gcaaggacgg gagtgagttt cccgtggaaa tcggcctcaa tcccatcaat accccggaag
      181 gattcatggt gctcagttcg gtggtggata tcacggcacg gaagcgatcc gaagcagaat
      241 tggaacggct caatcaacag ttgcgtatgc agaatcaaga actggaagcc tatgcctatg
      301 cggtgtctca tgatttgcgt gctcccttga gagctgttca caattatgca gactttttgc
      361 tcgaagattt atcctccaaa gtttctatag aagaaaagga gtatttgacc ggcattacga
      421 ccgccgtctg cgaagcggag gaattggttt ccgatctcct ggagctttca cggttgaatt
      481 accatgattc caatcctcaa ccttgctata ttggcaatct cattcataag gttcttcgaa
      541 cactcaattt cggggacgac gtacatgtcc aattgccgac ggagtggcca actatcctgg
      601 gcgaggaaca tctcctgaaa cagattttcc aaaatcttat ctccaacggt gtcaaattta
      661 accgctcttc acaaaaacac attgaggtgg actggcgtcc ggagagaaag agatttgtga
      721 ggtttttcat tcgtgataat ggaatcggaa ttttgccgca ataccaaaag caaatttttc
      781 agattttcca acgattgcac accaccaggg attatgaggg aaccggaatc ggcttggcga
      841 tcgccaaaaa agccgtgacc aaactcgggg gagagatcag ggtggaatcc gagaccggca
      901 aagggagcat cttttcattt accgtaccaa tgggagaaat gcatgatgat gaatgagtct
      961 cgccaaataa cgattctcat ggcggaggat aacgctcacg atgtcctggc aaccaagcgt
     1021 gcctggcacc aattcggttt cggcaatcat ctctatatcg tttcagatgg ggaggaatgc
     1081 ctggattatc tttatcatag agggaaatat caggactcga acatctacct tactccggat
     1141 ctcattcttc tcgacctcaa acttccgaag ctggacggcc atcaagtact acaaagtatt
     1201 cgtgcggata aaaaattccg ttttttgccc gtcatcatcc tgacgaattc cactctcgaa
     1261 accgaccgga ataaggggta tgaatacggt tgtactgcat tcctcaaaaa acctgtgggc
     1321 tattccaact ttgccaatct catcaaatcc attcgaacct tctggagttg tgtggaacca
     1381 tatgcccaac ctttaatgtg ctaaatagag tacacgacta tgtccattgt ccatccatcc
     1441 ggattatcgg cgctgctcat cgaagacgat acacacgaac gagcggccat ccgccgggct
     1501 ctccgaaatg atattcaaac tcttctcgaa tgcgaaaccg ccgaggaagc cattcgtcgt
     1561 ctcgaccaat ctccttcaga attcgacgtc atcatttccg acttgaaact gccgggaatg
     1621 gatggattga cgttcttccg gacccttcgc cggatgaagt gttcctctcc ctttatcctt
     1681 ttaacagcta atggatcaga acaaacggcg atagaggcgt tcaaagccgg agtggacgat
     1741 tacctaatca aagccacgca aagcggatat ctggaaatgc tctcgccctt aatcgctcaa
     1801 gtcgtccacc atcatcgcat gaagatcaga aacgaattat tagagctgtc tctcagggaa
     1861 gccaatgaac ggttccagca attcatggaa caatatcagg gcattttctg gttgactgac
     1921 tatgaagaac ctcgaaaggt attgtatgtc agcccgacct ataaaattat ctgggaccgt
     1981 tctgcccatc agctcaatca atattcatat gattggatgg aagcccttca tcccgatgat
     2041 cgaatccgcg tccggaccgc ttatctcgag cttgatgcca cccacaagta caatgaaact
     2101 tatcgaatta tcaaacccga tggctcatta cgctggattc atgatcgggg gttcctcatc
     2161 accgatccat ccggcaagcc gtatcgtatc gcacgactca ccgaagacat taccgaccgc
     2221 accacgatgt cggaagccct tcaagccagt gaggaacgct ttgccaaatt tatggctcac
     2281 ctgcctggct tagcctttat aaaagatgaa cattgccgtt tcatctattt gaatccggcc
     2341 ttcgaaaagg tctttaacat cacgattgca gactggatag gcaaaaccgt tcacgacgtg
     2401 cttcctcccg agacagcagc tcgcttacat accaatgaca aatgggtctt atctcatcgg
     2461 cagtctctgg caaccgtaga gaagatcccg cttcctgatg gttttcatca atatttaacc
     2521 gtgaaatttc ccatacttcg caatgaccgg tctcctttcc tgggaggagt atcaaccgat
     2581 gtcactgagc gactccaggc ggagacccaa cgagaccaaa ttttctcgct ttccccggat
     2641 ctcatgtgca tcatcggcat ggacggatat ctcaaaaaaa tcaatccggc tttcgaaaaa
     2701 ctattcgggt acaccgaaga ggagttcctt tcccagcctt tgatcgtgtt tacccatcct
     2761 gatgatcggc cgtccactct ggcggccttc catcaattgt ccgaggaaat taccgttttt
     2821 gattttcata accggcttcg ggacatacgc ggcaattatc gctggctgga atggcgggca
     2881 accgcgcaga tccaggagca ggccatttat gccgtggccc gggatatcac tcagcgctac
     2941 catgccaccg aagccctacg acgttacgaa caaatcgtct cgaccagttc ggattttctg
     3001 tgctttttag ataaagacga ttgttttcaa gctgtcaacc ggcgatttag tgaatttttc
     3061 aatctgtctg tgagagacat tatcggccgt caccttcgtg attttgtggg acaggatatt
     3121 tttgattccc tgaaatttca tttggaggat tgccgaaaag gacaggccgt tcggttccaa
     3181 cataaactga aattccccga cggaaatctt cgtttcatgg aagcccggtt gaatcctttt
     3241 ctggaacacg acggctctat tgcaggagtg actgcgacct ggagagatct gactgaacaa
     3301 aaatatctgg agcaacatat gaccca
//
LOCUS       DATIAL010000041         3304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_653018, whole genome shotgun sequence.
ACCESSION   DATIAL010000041 DATIAL010000000
VERSION     DATIAL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653018"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1190..1414
                     /locus_tag="VLA60_01320"
     CDS             1190..1414
                     /locus_tag="VLA60_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08021.1"
                     /translation="MACKRCGGLIVKESVYDREGSSQLVLMDRCLQCGNIEDPVSASN
                     RSKSPVPPSQKGKARRIPEHPRTPLEMAEG"
     gene            1417..3024
                     /locus_tag="VLA60_01325"
     CDS             1417..3024
                     /locus_tag="VLA60_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HSF08022.1"
                     /translation="MELRRHQKELADICDQILEGRGLTDIVCAVTPGGGKSLLPQILA
                     SRLIPAIADALCWIVPRSVLQDQGARGFQDPSHRALLGHRLEAMMTTNQEHPTRGCAA
                     YVTTYQALAADTRKINAKEFRRKRYILVLDEPHHLEEGGLWHEAIQPLYDRAVLRVLM
                     SGTFERGEGTPIAFLPYSTTDRGNRLDWDSTESRAVIHYTLADALREQACIDLTVHYA
                     NCQATWLDAQGTEHHVESLAHAGKQTAAALLTALKTEAALELLSTGVKDWQVHRTTHS
                     RSKLLVVAANIEQAQKYTAWLQERGVPARIATSEDSTAAYQAIKAFKRQGSGAVDCLI
                     TVAMAYEGLDVPAITHLICLTHIRTKPWIEQVVHRATRMDPLAGPYAEQRAYIYAPDD
                     RPFRECLGYILSQRTTAIANSPSSTQVLGEGAYPDAQGLLPTDRAESSIQPLRSGVLE
                     MRNQGLWANLATQGQTEIHQETPKERLDRLRKTIEQHVRAHEQSRKLSHGTVNRAVYS
                     KFRKSRAAMTESELHKVMQWVHKIYPL"
     gene            3054..>3304
                     /locus_tag="VLA60_01330"
     CDS             3054..>3304
                     /locus_tag="VLA60_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08023.1"
                     /translation="MSIQWFPGHMNVARKEAAKAMEAIDVLVEILDARMPDASSNPLI
                     TELRLHRQRPCLKVLNKADLADPTVTQAWLTEFNREPDV"
BASE COUNT          811 a          928 c          882 g          683 t
ORIGIN      
        1 gtcaatctca gttcctgcat ggcccggtcc aatggtcctt ccttgagaag ggacagaccc
       61 aactgtcgtt gagccggtct aggtttcctc atcgccctca agacaattgg tacattcatc
      121 caattttggg tcaccatgac gactacaaaa atgacgggcg cattgccggc aggtcataaa
      181 actcatgcga attaaatccg cattctcaca cacataacaa ttcttttctc gtttcgcttt
      241 tggcatcatt cttctccttg ggtagcaaac ttctaaaaac atctcaagac atggggcgca
      301 caagccacgc ccctaagaca tagatacggc gccaacgatt tcagtgtgtc atggccaagc
      361 gtggccttgg accatcttca tgaaattggc atgaatgtca gggagggcgt gcatctcgca
      421 atgacgaggg gagcggacgc tgatccgtaa cagggagggg gagcctaccg catgatcgaa
      481 cttcatggca atgcgtaacg aatctgtgac acctctagcc cttctacggg atggagggcg
      541 gacaaaacct ccaggggcct tggcctcctg cacaggatgg gtttacaatc cctcccctac
      601 gttcaaaaga catctcagcc catctgcgaa aagtcaattg ggattgcgat cctacggatc
      661 gctcaatttt taaggcagcg cgtcattatt cgattgcaaa ttgtcgacat tttgggtatg
      721 tctatccatc ctgaagtggt ccccgtatgg aaaggttctc acttgttcaa cgatcttcac
      781 gaaaggcaaa ggcagaaatt ggtacagatc aggtaattcc cctacggtgg tttttatgag
      841 tatggccaca tgccggcaaa gagcgtgaca ctgacccagt gtttccgggt ccatgccatc
      901 aacagttgga tccctaagag agggaaaacc gcttcgctct ccatgtgcat ttcacaatca
      961 tcaattgcaa ttttctcaat ggttcacgga ggagtgggga acaaaaaacc ttccggtacc
     1021 tctctgtcac ttgtgtatcg gtcggctgtt tctcaatagg ggtggtttcg ggcttcagtc
     1081 gtcggtaatg cccgacataa gatcagggaa gatctcaggg aaggactcga acagcatgag
     1141 gcaccaccct gtcacaacac atgtcttttt gaagaaagga aagttcttta tggcctgtaa
     1201 gcgatgtggc ggactgattg tgaaagaatc cgtctatgat agagaaggca gcagtcagtt
     1261 agtcctcatg gaccgttgcc tccaatgcgg caatattgag gatcccgtta gcgcgagtaa
     1321 tcgctcgaaa tcgcccgttc ctccatccca gaagggcaaa gcccgccgca tcccagagca
     1381 tcccaggaca ccgctggaga tggccgaggg ataagcatgg agctacggcg gcaccagaag
     1441 gaattagcgg acatctgcga ccaaattcta gaggggcggg gtctgacaga cattgtctgt
     1501 gctgttacac cgggtggcgg caaaagcctg ctgcctcaga tcctggcctc ccgcttgatt
     1561 ccagccattg ccgacgcctt gtgctggatt gtcccacgca gtgtcttaca ggaccagggt
     1621 gcgcgagggt ttcaggatcc cagccaccgg gctctcttag gccatcggct ggaggccatg
     1681 atgaccacca accaggaaca tccaacaaga ggatgtgcgg cctatgtcac gacctatcag
     1741 gccctggctg ccgatacccg caaaatcaac gccaaagaat tccgccgtaa gcggtatatc
     1801 ctcgttctgg acgaaccgca tcatctcgaa gagggtggac tgtggcatga ggccattcag
     1861 ccactctatg accgggcggt tctgcgagtg ttgatgagcg ggaccttcga acgtggtgaa
     1921 ggcacaccaa tagcctttct gccgtattcg accaccgacc ggggaaaccg gcttgactgg
     1981 gacagcacgg aatcccgggc cgtcatccac tacaccttag ccgatgcgct gcgcgaacag
     2041 gcctgcattg atttgaccgt ccattacgcg aactgtcagg caacctggtt ggacgctcag
     2101 ggaaccgagc accatgtcga aagcctggcg catgccggga agcagacggc agcggcactc
     2161 ctgacggcac tgaagacgga agcagccctg gaactcttga gcacaggcgt gaaggattgg
     2221 caggtacatc gcaccaccca ttcccgatcg aagctgctgg tggtggccgc gaacattgag
     2281 caggcgcaaa agtatacggc ctggctccag gaacgaggcg tgcctgccag aattgccacg
     2341 agtgaggatt ccacggcagc ctatcaggcc atcaaagcgt ttaaacgaca gggtagcgga
     2401 gccgtggact gcctgattac ggtggccatg gcctatgaag gcctggatgt gccggccatt
     2461 acccatctta tctgcttgac ccatatccgg accaagccat ggatcgaaca ggtcgttcat
     2521 cgagccacac gcatggatcc cctggcggga ccttatgccg agcaacgggc ctatatttac
     2581 gctccagacg accggccatt ccgggaatgt ctgggataca ttctgtccca acgcacaacc
     2641 gcaatcgcca acagtccctc atcgacacag gtcctcgggg aaggggcata ccccgatgca
     2701 cagggcctcc tgccgacgga tcgggcggaa tcaagcatcc aacccctgcg gtccggcgtc
     2761 cttgaaatgc gcaatcaagg gttgtgggcg aacctggcga cacagggcca aacagaaatt
     2821 caccaggaga ccccgaaaga acgattagat cgattgcgaa aaacaatcga acagcatgtc
     2881 cgcgcgcacg agcaatcgcg gaaactgtca catgggacgg tcaatcgggc ggtctattcg
     2941 aaatttcgga aatcccgtgc cgccatgacc gaatctgaac tccataaagt catgcaatgg
     3001 gttcataaga tctatccgct ctgagatcat ttttaccagg aagcctcgtc cctatgtcca
     3061 tacaatggtt tcccggccac atgaatgtcg cgcgcaagga agcggccaaa gcgatggaag
     3121 ccatcgatgt cctggtcgaa atactggatg cgcgcatgcc ggacgccagc agcaacccgt
     3181 tgatcacgga actgcggctg caccgccagc gcccctgctt gaaggtgctc aacaaagccg
     3241 atctcgccga ccccaccgtc actcaagcct ggctgaccga attcaaccgg gaacctgacg
     3301 tcaa
//
LOCUS       DATIAL010000042        15367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_868427, whole genome shotgun sequence.
ACCESSION   DATIAL010000042 DATIAL010000000
VERSION     DATIAL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868427"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1855
                     /locus_tag="VLA60_01335"
     CDS             <1..1855
                     /locus_tag="VLA60_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSF08024.1"
                     /translation="VMRRSETREGNRRWYTVMVAHEVGHVEFGTYALSTEVLQRVATR
                     VQTRYHEEVPSPNRAIHTLGNLFQRYPQPEIIRDLWEIVEDGRIDFLLRHEYPGLQED
                     LTSLTKEAMELRTLSHGMTAREMVLDALLLLYAGFTKEEFTRPGLQEVIDEIWQIART
                     ILHPTATVDESVELADRLYQELERRIGTLGKHNQEPEPFSNTSGVSDSGGQPEAAEHL
                     EEAYQPLSNWGYRGILNPDHVKGGEEGEQGSKGQTGGQQDQVVTIGENAGGDTPSKFE
                     RRSPQQPDPSQDPKKPTFGESPMKQWFQPTFRPSDGQQGARLREGEYLYEEWDGTVRD
                     YRPQWCRVIEQAGREGSPDFVDETFQTYGPMVRLIRRYFEAIRPEAFRRMGRQSHGED
                     IDLDALVNWMVDRRHGNDSSDQVYATRQKRDRQVAVAFLVDMSGSTGRQIGTRARPVI
                     DIEKEGLLLLSEALSAIGDQYAIYGFSGQSRQSVDIHVLKDFDQRPGGRVGLKISGVT
                     SKQQNRDGAAIRHATHRLKQQAAKVRLLILISDGKPLDDDYADEYSLEDTKMALREAR
                     LQGVHPFCITIDQAPTDYVKRMYGEIGYVVVDEVESLPMKLPKIYQRLTAR"
     gene            1873..2283
                     /locus_tag="VLA60_01340"
     CDS             1873..2283
                     /locus_tag="VLA60_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08025.1"
                     /translation="MTSLPSHSDVPPWLYKLFTGHQYPYVRRQAKFDRKDRQEDGERF
                     EPTQDDIREKFWEIFPRCSTKMLQEVKVGMIVAFKELGGYEAGTYQEFIDHPEEFLSR
                     EYGKKKIKVNFYDGDNFVCTINFKVAGWTSHDDD"
     gene            2326..3273
                     /gene="mdh"
                     /locus_tag="VLA60_01345"
     CDS             2326..3273
                     /gene="mdh"
                     /locus_tag="VLA60_01345"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HSF08026.1"
                     /translation="MSRIKVTVVGAGNVGGSIAQRLAEKNWYDIVLVDIVEGLPQGKA
                     LDLVEAGPICAYDSAVTGTNHYEATKDSDVVVITSGVPRRPGMSRDELLETNTKIVST
                     VVRETAKRSPDAIFIIVSNPLDVMTHVAHRVSGFPRNRVIGMAGVLDSARFRSFIAEA
                     LQVSVENIHAMVLGGHGDSMVPLNRYTTVAGRPVTEWLSAEALEALIKRTREGGIEIV
                     NLLKTGSAYYAPAASVVEMVEAISKDQKKILPCAALCAGEYGFNDLFLGVPVKLGAGG
                     AEEIIEYALTPEEHAALKVSAEAVKELCGHVDQMMKKTL"
     gene            3546..4841
                     /gene="nuoF"
                     /locus_tag="VLA60_01350"
     CDS             3546..4841
                     /gene="nuoF"
                     /locus_tag="VLA60_01350"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HSF08027.1"
                     /translation="MDNAFAPRVLREVKGEPEDIKAYRQQGGYEAWIQCVTKNDPQAI
                     IAQLKEAHLRGRGGAGFPTGLKWDKVVNHRVKERYFVCNAGEHEPGTFKDRYLLRHHP
                     HQLLEGCLIAAYTVGAKAAYIYLNHEFSEERAIFERAKEQATEQGLLGKNFLGTGLTL
                     DIEIFDGYGSYVAGEETAMLESMQGRPAQPKQKPPFYPTEFGLHGKPTLVNNVETLSN
                     IPQLLRNGPEWFRQVGTSTTPGTMLFSLSGAVNRPGVYELPLGTSIRHLIEVCGQGVP
                     NGHGVKAVFPGGPSFSMVGADQLELPMDFDSLKKAGTGLGSAGVIIVDDATCMVEQTL
                     KFSGFFERESCGQCPPCRIGTQELAILMRKIEDGSGEERDLAKLLQICGFVKGTGFCT
                     LVTGAAVLVQNSLRLFRHEFEDHIRLGRCPFKPVPVGAK"
     gene            4854..5180
                     /locus_tag="VLA60_01355"
     CDS             4854..5180
                     /locus_tag="VLA60_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSF08028.1"
                     /translation="MPRVTFLHPEGKSGEVSEGLSLLEVAEKLGFPLNHDCGGNASCT
                     TCRVDVIAGEGNLSDIDFDEQDLLDREALTEPYHRLGCQARVFGDVIVQVPEEKWGEA
                     EIERSA"
     gene            5286..5747
                     /locus_tag="VLA60_01360"
     CDS             5286..5747
                     /locus_tag="VLA60_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HSF08029.1"
                     /translation="MPTASSITRTYRFCAAHRLHTDLLPDDTNKKIFGKCNNLNGHGH
                     NYTVLVTVAGELDMGTGLITNVDALDQLVKEKIVDRFDHQHLNFDPAFAQVTTTGENL
                     ARLIWDLLVDQMPSGRLEKIGVIETRDNFFEYIGTLRVPSDSSNRNGIKEN"
     gene            5822..6400
                     /gene="folE"
                     /locus_tag="VLA60_01365"
     CDS             5822..6400
                     /gene="folE"
                     /locus_tag="VLA60_01365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HSF08030.1"
                     /translation="MPNLPNLEIIVKQLLENLGENPQRHGLTDTPKRVAKSMAFLTKG
                     YQQDIDELLNGALFPIVYDEMVIVRDIDFFSLCEHHLLPFFGKCHIGYIPNKHVVGLS
                     KIPRIVDAFSRRLQVQERLTVQIAHTLHTKLKPRGVAVVMEARHLCMSMRGVEKQNTV
                     AVTSEMLGVFRNQEQTRNEFLKLIRQRGCDGD"
     gene            complement(6731..7525)
                     /locus_tag="VLA60_01370"
     CDS             complement(6731..7525)
                     /locus_tag="VLA60_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF08031.1"
                     /translation="MKALIHGVTVGYSDQGNGTPLVFIHAFPLSKTMWQPQVDALKDT
                     YRVITIDLGGHGESDIVPWNDSLDDYAKDVIHLLDHLGIAQAVFVGLSMGGYTLFAIY
                     RHYADRVKAMVLADTKAQADSEEGKAGRGSMAQVAFSDGAPAIADLMLPKLLSPSTIE
                     RHSEMVEQVRQMILQTSTGGIVVDLVAMAARPDSTDLLSTITCPTLVIVGENDVATPA
                     SESEYMAKRITGSILVTIPEAGHLSNLEQPAAFNEALRNFLTTHRL"
     gene            7700..9022
                     /locus_tag="VLA60_01375"
     CDS             7700..9022
                     /locus_tag="VLA60_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HSF08032.1"
                     /translation="MESFESQSQADLFPVQESSDIRDAPLAERMRPLDFDEFVGQEDV
                     LAEGQPLRVSIEHDRVPSLILWGPPGTGKTTLASLIAKKTQATFVPFSAVLSGVPELR
                     GLLKTAQQRRKVSGQRTLLFVDEIHRFNKAQQDAFLPYVERGDIILIGATTQNPSFEV
                     IAPLLSRSMVVVLQSLNEEALRHILNRALSDTKRGLGSSRVTLTPDAQRLLIGYGNGD
                     ARSMLTGLEFVVGQYAKPGKSTTIDQPHVEQALKQKALRYDRDGEEHYNLISAFIKSM
                     RDSDPDGALYWLARMLEGGEDPKFIARRMMIFASEDIGMADPQGLLIASAVFHAVEVI
                     GLPEAQINLAHGVTYLATAPKSNASYMGLLAARKDAKEKGNLPVPLHLRNAVTSLMKD
                     LDYGKNYRYAHDDPSGAKAQTHLPKELEGRKYYHHKIPSSPSSNLDEE"
     gene            complement(9433..10182)
                     /locus_tag="VLA60_01380"
     CDS             complement(9433..10182)
                     /locus_tag="VLA60_01380"
                     /inference="COORDINATES: protein motif:HMM:NF022633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HSF08033.1"
                     /translation="MNLKAQAVYFSSPLNFNDPYDCAITASISDPTPDELQRVLHHYL
                     DDSDVPPELKTQLSTFSQPELKENIRNGAMLALNENREKFLKKNGVACFSERNDNLLM
                     WSHYGGQYKGLCLEFRTEHEPFNKLLPVKYVERMPQIRIDSILIDNDPGQLIDLFCTK
                     SEAWAYEKEWRGLHTEVGTLFGYRSPALKAVYFGPDIERGALEIVCLILRGQNPDVEF
                     WQGSRSQSEFRVHFEHFTYTPYIDAKQNGLA"
     gene            complement(10401..12263)
                     /locus_tag="VLA60_01385"
     CDS             complement(10401..12263)
                     /locus_tag="VLA60_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSF08034.1"
                     /translation="MLHIEQISKSYPTKPLFIEASAHLKPNTRVGLVGPNGSGKTSLM
                     RMILNEEEIESGKIRQRPFLRLGYLPQELESLPGNTILDATHRNQFPDHEAKRILAGL
                     GFQESDWERKIDTLSGGYRMRVALAHLLLSAPDVLMLDEPTNHLDKPTQRWLESFLLS
                     SNFTLLIISHDIGFLDNMVTHIWEVRNHTLQEYRGNYTRFQKLRAERDAQEEAAANRQ
                     SKEIARVQNFVDRFRYQANKASQVQSRIKQLEKVKRIELQRDTKRLRFKFPDPPASGK
                     DVFELRDIGKRYGDKIIYDGLDFSVERGQRVALVGENGAGKSTLLKILAGVLEFEKGS
                     RTLGHGVTLHYFAQHQAEILKSEHSILDSLEEAAPMTERNFLRGLAGAFLFSGDDQKK
                     PIKALSGGERNRVALARMLVEPANTLLLDEPTNHLDPTSVDMLTDALLQFPGTMVFIS
                     HDPTFLMRVATRVVEIDDGQARDYIGDYEYYCWKRAKELEDQIPPEATVDKKKSKKKE
                     KPQKEKPTTEHSTNGTARTTVSPPDGPSRRDLSKSIARLEKQVTTVEKDIAQLEEQIK
                     ARDVELADPATYQDYSRWNSLHHEREQWGHDLDRLTHKWADLGAQLEKQQSQIS"
     gene            complement(12348..12423)
                     /locus_tag="VLA60_01390"
     tRNA            complement(12348..12423)
                     /locus_tag="VLA60_01390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12388..12390),aa:Ala,seq:ggc)
     gene            12608..13915
                     /locus_tag="VLA60_01395"
     CDS             12608..13915
                     /locus_tag="VLA60_01395"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HSF08035.1"
                     /translation="MAALVVVGSQWGDEGKGKIVDLLAKDADIIVRYQGGSNAGHTVI
                     TNKGTFIFHLIPSGILYRGKVCALGNGVAIDPAGLIEELDRLTTKGIPVGKRFLISNR
                     AHVIMPYHKAIDKAEEQAKGARRIGTTGRGIGPAYVDKMARVGIRMGDLLNPDVLQQK
                     IRENLVEINAFLERIYNVDGFTVEKVFKDYQAYGERLRPHITDVALVLDRAFERGKRV
                     LFEGAQGTHLDVDFGTYPYVTSSSSCSGGACTGSGVGPTTITGVLGVTKAYTTRVGGG
                     PFPTELTDEMGQQLMQRGNEYGSTTGRARRCGWLDMVLLRYAVRINGLTSLAITKLDV
                     LDGCKELKICTGYRYQGKLHKEMPGDLQVLAGCEPVYERWSGWTTDTTGLKGYKGLPP
                     AAKRYLARIEELTGCRIDMISTGSKREDTIVVKNPLIRSRKGS"
     gene            13991..14066
                     /locus_tag="VLA60_01400"
     tRNA            13991..14066
                     /locus_tag="VLA60_01400"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14024..14026,aa:Lys,seq:ttt)
     gene            complement(14230..>15367)
                     /locus_tag="VLA60_01405"
     CDS             complement(14230..>15367)
                     /locus_tag="VLA60_01405"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF08036.1"
                     /translation="GSLFTNAMLYSLGDLILALCRPDVAEQLAVIRREDPAKIPKAEL
                     TLLGRPLHVIAAAMAKHWNLPDSLVQLLEKKPVWPKSRPEADQQIMEGIVRAANELSY
                     CLLNPLAAGQGEVLQGLIEQFLPPFGLSMIQLEHTVSKAFSQASEIASAINIDRQHFL
                     PASEADELFLHNPQLHRLTQTIHQVLDPEGVLSRELATSAQQASPSLQPSPASDRPLL
                     DFTIQSMKISEPSLLLTFVTRTLHASYGFERVWLALVVPGKDMLQAKIGYGPHVETIQ
                     PVFHCPLSHGNFWDRLLHSFHPIQYSSLVQEGESGGMPTAFLKYWGNQPGFAGTLYAP
                     NRPIGVILVDRYSTGNPLTDTDFAAFALMLSQANANLARLAHKD"
BASE COUNT         3739 a         3444 c         4299 g         3885 t
ORIGIN      
        1 ggtcatgagg cgatcagaga cccgtgaagg aaaccgacga tggtatacgg tcatggtcgc
       61 tcatgaagtt gggcacgtgg aatttggaac gtatgcgctc tcgaccgaag tgttgcaacg
      121 tgtggctacc cgggtgcaga cccgatacca cgaagaagtt cctagcccca atagagccat
      181 tcacacattg ggaaatctct ttcaacgcta ccctcaaccc gaaattatcc gggatttgtg
      241 ggaaattgtg gaagatggac gaattgactt tttgctccgt catgaatacc caggattaca
      301 agaggatttg acgtctttga caaaggaggc catggagctt cgcacgcttt cccatgggat
      361 gacggctcgt gagatggtgc tggacgcatt gttgttgctg tatgcagggt tcaccaagga
      421 ggaattcact cgtccgggac ttcaagaggt gattgatgaa atctggcaga ttgcccgaac
      481 gatcttacat cccaccgcca ccgtcgatga atctgttgaa cttgccgacc ggctctacca
      541 ggaattggag cgccggattg gaaccctggg aaagcacaat caggaacctg aacccttttc
      601 caatacttcc ggggtttctg actcaggtgg ccaacctgaa gcggctgaac acttggaaga
      661 ggcgtatcag cctttgagta actggggata tcggggcatt ctcaatccag accacgtcaa
      721 aggaggggaa gagggggaac aggggtcgaa agggcagaca ggagggcaac aggatcaagt
      781 tgtcacaata ggggaaaatg ctgggggaga cacacccagc aaatttgagc gtcgatctcc
      841 tcaacaaccc gatccttcgc aagatcctaa gaaaccaacc tttggtgaat cacctatgaa
      901 gcagtggttt caacctacgt ttcgtccgag cgatggacaa cagggggctc gtctccgtga
      961 gggagagtat ctgtatgaag agtgggatgg gacggtccgt gactatcgac ctcaatggtg
     1021 tcgtgtgatt gaacaggcag ggcgtgaagg gtcaccagac tttgtagatg aaacgtttca
     1081 aacgtatggc ccgatggtgc gactcatccg ccggtatttt gaagcgattc gtccggaagc
     1141 cttccgtcga atggggcgcc aatcacatgg ggaggacatt gatttggatg cgttggtgaa
     1201 ttggatggtt gatcgacggc atggaaacga ttcttctgat caggtgtatg ccaccagaca
     1261 aaaacgtgat cgacaggttg cggtggcatt tctggtggat atgagcgggt cgaccggacg
     1321 gcagatcgga actcgagccc gtcccgtcat cgacattgaa aaggaaggct tgctcctgct
     1381 ctcagaagcg ttgtctgcga ttggcgatca atatgcgata tatggatttt ccgggcaatc
     1441 ccggcaatcg gtggacattc acgtgttgaa agattttgac cagcgacctg gggggcgtgt
     1501 cggtttgaaa atcagcggag tgacctccaa acagcagaac cgtgatggtg cggcgatacg
     1561 acatgcgacg catcgattga aacagcaagc ggcaaaagtc cggcttctca tcctgatcag
     1621 tgatgggaaa cccttagatg atgactatgc ggatgaatat tccctggaag acaccaaaat
     1681 ggctcttcga gaagcccgtc ttcagggcgt ccatccgttc tgcattacca ttgatcaagc
     1741 tccaaccgat tatgtgaaac ggatgtatgg ggagattggg tatgtggttg tcgatgaagt
     1801 cgaatcactt ccgatgaaat tgccaaaaat ttatcaacgg cttacggcca gatgagcgga
     1861 gatgggagat gtatgaccag tttaccttca cactctgatg tgccaccgtg gctctataag
     1921 ttgtttaccg gccatcaata cccctatgtc cgacgccagg cgaagtttga ccggaaagat
     1981 cgtcaggagg acggagaacg gttcgagcca actcaagatg atatccgtga aaaattttgg
     2041 gaaatctttc ctcgctgttc gaccaaaatg ctacaggagg tcaaggtcgg gatgattgtc
     2101 gcgtttaagg aattaggagg gtatgaggcg ggaacctacc aggaatttat cgaccatccg
     2161 gaagagtttt tgagtcggga atacggaaag aaaaaaatca aagtcaattt ttatgatggg
     2221 gataattttg tgtgtacgat caactttaaa gtcgcagggt ggacgagcca tgacgatgat
     2281 taaacccgca tggctgggaa ccctttcaaa gggatgggtg gtttcatgag ccgtatcaaa
     2341 gtgactgtgg tgggggctgg aaatgtgggg gggtccatcg cgcaacggtt ggcagaaaaa
     2401 aattggtacg acattgtgct ggtcgatatc gtcgagggtt tgccgcaggg caaagcattg
     2461 gacctggtcg aagccggccc aatttgtgcc tacgattccg ccgtcacagg tacgaatcat
     2521 tatgaggcga ccaaagattc agacgttgtg gtgatcacgt caggagtgcc tcgccggcca
     2581 gggatgagcc gggatgaatt attggaaacc aataccaaaa tcgtgtccac cgtagtccgt
     2641 gagacggcca aacgttcccc tgatgccatt ttcattattg tctccaaccc tcttgatgtg
     2701 atgacccacg tcgcgcatcg ggtgagtgga tttccaagga accgggtcat tgggatggcg
     2761 ggggtgttgg attccgcccg attccggtcc tttattgcgg aagccttgca ggtctcggtc
     2821 gaaaatattc atgcgatggt cctaggagga catggcgatt cgatggtgcc cttgaatcgg
     2881 tataccacgg tggcggggag gccagtgacc gaatggttgt cggcagaggc attagaggcc
     2941 ctgatcaagc ggacccgcga gggaggaatc gaaatcgtca atctactcaa gacagggagt
     3001 gcctattacg ccccggctgc ctcagtcgta gaaatggttg aagctatctc taaagatcaa
     3061 aagaaaatcc ttccctgtgc cgcgttatgc gcaggggaat atggttttaa cgacctcttt
     3121 ctgggtgtgc cggtgaaact aggggcaggc ggagcggaag agattattga atatgccctc
     3181 acccccgagg aacacgcggc tctgaaggtt tcagccgaag ccgttaagga attgtgcggc
     3241 catgttgatc agatgatgaa aaagaccctc tagcgtgatg gatggcagga tgaacttgcc
     3301 catctttttt cttcactaaa tagacgtgcc ttctggtcct cttattacct tgacgacttt
     3361 agcgacccat tcgtatagtg ggtggtgctc gtacgctgaa cccgccttga cgcctgattt
     3421 cccttaggaa tatggaagga aatttggttt ttcgaggaag gaatttgtaa aaaagaccat
     3481 catccaaatg ttgaacataa tggaaaaacg catgtgggtc caaaccattg atcaaggggg
     3541 cttcggtgga taacgcattc gcacctcggg tgttgcggga ggtaaagggt gaaccagagg
     3601 acataaaggc gtaccgccag caaggggggt atgaagcatg gattcagtgt gtcacgaaaa
     3661 atgacccgca ggccataatt gcacaattga aagaggccca tctgcgagga cgggggggcg
     3721 ctggatttcc taccggactg aaatgggaca aggtggtcaa tcatcgcgtt aaagagcgat
     3781 actttgtctg taatgccggg gagcatgaac ctgggacgtt caaagatcgg tatttgcttc
     3841 gacatcatcc tcaccaactt ctcgaaggat gcctgattgc ggcgtatacc gttggagcga
     3901 aggcggcgta tatctattta aatcatgaat tttccgaaga gcgagccatc tttgaacgag
     3961 ctaaggagca ggccacagag cagggcttat tgggaaagaa ttttcttggt acaggcctca
     4021 ccctggatat tgaaattttt gatggatatg gcagttacgt ggcgggtgaa gaaactgcga
     4081 tgttggaatc gatgcagggg cgtccagccc agcctaagca aaagccacca ttttatccca
     4141 cggagtttgg actgcatggc aagccgacct tggtcaataa tgttgaaacg ctgtcgaata
     4201 ttccccaact cttgcggaac ggcccagaat ggtttcgtca ggtcggcacc agtactactc
     4261 cagggacgat gttgttttcg ctcagtggag ccgtgaaccg gcccggtgtg tatgaactgc
     4321 cgttaggaac gtccatccga catctgattg aggtctgtgg gcagggcgtg ccgaacggac
     4381 atggtgtcaa agccgtgttc cccggtggac catccttttc catggtgggg gcggatcaat
     4441 tggaattacc catggatttt gattcgctta agaaagccgg tactggattg gggtctgccg
     4501 gcgtcattat tgtcgatgat gcaacatgta tggtggagca aacgctcaag ttttccggat
     4561 tttttgaacg ggaaagttgt gggcaatgtc caccttgccg gattggtacc caggaattag
     4621 cgatcttaat gagaaaaatc gaagatggat caggagaaga gcgcgatctg gccaaactgt
     4681 tgcaaatttg tggatttgtc aaaggcaccg gattttgtac tctggtgacg ggagctgcgg
     4741 tattggtaca aaatagctta cgtctgtttc gtcacgaatt cgaagaccat attcgtctgg
     4801 gacgatgtcc gttcaagccg gttccagttg gagctaagta aggaggagac gcaatgcctc
     4861 gcgttacatt tcttcatcca gaagggaagt ctggcgaagt ttctgaaggc ctttccctct
     4921 tggaagtcgc ggagaaactc gggttcccat tgaatcatga ttgtgggggc aacgcctcct
     4981 gcacgacttg ccgggtggat gtgattgcgg gcgaagggaa tctttctgat atcgattttg
     5041 acgagcagga tttgctcgat cgggaagccc tgactgagcc ttaccaccgg ttaggctgtc
     5101 aggctcgagt attcggggat gtgattgtgc aagtccccga agaaaaatgg ggtgaggccg
     5161 aaattgaacg atctgcgtga attggccatt ttctcaagag taaagggaga gagtgtgggt
     5221 acccgccaca gccgcattat tcagaacatg ccttgcaatg tgaaagaatc ctgagaccga
     5281 ctgctatgcc gactgcatcg tccatcacca gaacctatcg gttttgcgcc gcccatcgtc
     5341 tgcataccga tctattgcca gacgatacca ataagaagat ttttggaaaa tgtaataacc
     5401 tgaacgggca tgggcataat tatacggttc ttgtgacggt ggccggagaa ttggatatgg
     5461 gcacagggtt gatcaccaac gtggacgcct tggatcaact cgtgaaagaa aagattgtcg
     5521 atcgttttga tcatcaacat ctcaattttg atcccgcttt tgcacaagtc acgacaactg
     5581 gtgaaaattt agcgcgtttg atttgggatc tactggttga tcagatgcct tccgggcgat
     5641 tggaaaaaat tggggtgatt gaaaccagag ataacttctt tgaatatatc ggcactctca
     5701 gagtgcccag cgattcttcc aacagaaacg ggataaagga gaactaatgc cgaccaagcc
     5761 gaagcggccc tcatccatca agccacgggc ggtcgatcaa gacaacagcc agccggttcg
     5821 aatgcccaat ctcccgaacc tggagatcat tgtaaagcaa ctactggaaa atttgggtga
     5881 aaatccccaa cggcatggtt taaccgatac gcccaagcgg gtagccaaat ccatggcctt
     5941 tcttaccaaa ggctatcaac aagatatcga tgaattattg aatggggctc tgttccctat
     6001 cgtatatgat gagatggtta tcgtgcgcga tatcgatttt tttagcctct gtgagcatca
     6061 ccttctgccg ttttttggga aatgtcatat cgggtatatt cccaataagc atgtggtggg
     6121 cttgagcaaa atcccacgga ttgtcgatgc ctttagtcgt cggctgcaag tccaggaacg
     6181 gttgacggtc cagattgccc acacccttca cacgaagctc aagccacgtg gggtggctgt
     6241 tgtcatggaa gcccgccatc tgtgtatgag tatgcgtggg gtagaaaaac agaatactgt
     6301 ggcggtcacc agcgaaatgc tcggggtgtt tcgcaaccaa gagcaaaccc gaaacgaatt
     6361 ccttaagctc attcgccagc gcggatgcga cggcgattga aattttccct ccatttcatc
     6421 tttccccttc aaaaagagct gatggctgga cttttctaga taattgattc ttataatgga
     6481 atcaggttta atgcgacatt attgagtgtt catatgtgaa cccaaggaag ggcatgtcgc
     6541 gcaggaggcg ccggtctcat ccttatattt ctttctggtt tactattggg tctggggcga
     6601 gagaacctct ttttgatttt ccctcaattt cctttctgtt aagtccgttc tttctctatc
     6661 tctttagttt caacccaact ccttccagta tcggatgttt ttctgatccg tctggccttg
     6721 ccggcagtac ttataaccgg tgtgtcgtga gaaagttccg gagtgcttca ttgaacgcgg
     6781 cgggttgttc caagttcgat agatggccgg cttcagggat ggtgaccaag attgagccgg
     6841 tgatacgttt ggccatgtac tcagactctg aggccggggt cgcgacatca ttctcgccca
     6901 caatgaccaa agtcgggcag gtgattgtgg ataaaaggtc tgttgaatca ggtctagcgg
     6961 ccatcgccac caaatcgacg acaattcctc ccgttgatgt ttgaagaatc atctgccgaa
     7021 cctgctccac catttcagaa tgacgctcaa tggtggaagg ggaaagaagt ttaggaagca
     7081 tgaggtcggc aatggctggt gcgccatcgc taaaagctac ttgagccatc gacccacgtc
     7141 cggctttccc ttcttcacta tccgcttgcg cttttgtatc tgccaaaacc atggctttta
     7201 cccgatcagc ataatgtcga tagattgcaa agagggtata tccgcccatc gacaagccca
     7261 caaacactgc ttgagcaatc ccgaggtgat cgagcaggtg gatgacatcc ttggcataat
     7321 cgtccaacga atcgttccat ggcacgatgt cggattctcc atgtcctcca aggtcgatgg
     7381 tgatcactcg ataggtgtcc ttcagcgcat cgacttgagg ttgccacatg gttttggaaa
     7441 gtggaaaggc atggatgaag acgagcggcg tgccgttccc ttgatcggaa tacccgacgg
     7501 taactccatg aatcagcgct ttcaccgttc tttccttttt tgtcgatggg agccacctcg
     7561 atttttctcg agtgatgtca tcaaagagcc atatgctgat ttgatttgcg ggtgcatgac
     7621 cggtggttat agcatagtaa gcctatagag gtaatcatag attcggcccc cagaaatgcg
     7681 taggggtaag tgcattttta tggaatcctt tgagtcacag tcccaagcag atcttttccc
     7741 tgttcaggaa tcttccgata tacgcgacgc tccgttagcg gagaggatgc gtccgctgga
     7801 ttttgatgag tttgtgggac aggaagacgt cctggccgaa ggtcagccgc tacgtgtgtc
     7861 cattgaacat gatcgtgtgc cctcacttat tttatgggga ccgccgggga cgggaaagac
     7921 gaccctagcg agtttgattg ccaagaaaac ccaagcgaca ttcgtcccgt tttcagcggt
     7981 gcttagtggc gtgccggagt tgcgcggatt gctgaaaacg gcccaacaac gccgaaaggt
     8041 gtcaggacag cgtacacttc tttttgtgga tgaaatccat cgattcaata aagcgcaaca
     8101 ggatgccttc cttccctatg ttgaacgggg agatatcata ctcattggag ccaccacaca
     8161 gaatccttcc tttgaagtga ttgctccctt gttgtcccgg tctatggtgg tggtgcttca
     8221 atccttaaat gaggaggcgt tgaggcatat cttgaatcgt gccctttcgg atacgaaacg
     8281 ggggctcggt tcctcacgcg tgacccttac tcccgacgcg caacgtctgt tgattggata
     8341 tggcaatggc gatgcccggt cgatgttaac gggcctggaa tttgtcgttg gccaatatgc
     8401 aaagcctggc aaatctacga ccattgatca accgcacgtt gagcaagccc taaagcagaa
     8461 ggccttacga tacgaccggg atggagaaga gcactacaat ctgatttcgg cctttattaa
     8521 aagtatgcgc gattccgatc ccgacggggc gttgtattgg ctggcgcgga tgctggaagg
     8581 cggagaagat cccaagttca tcgccaggcg catgatgatt tttgcgtcgg aagatatcgg
     8641 gatggccgac ccccaggggc tcttgattgc ctcagccgtt tttcatgcgg tggaggttat
     8701 cgggttacct gaggcgcaaa tcaacttggc gcatggggtg acctatttag ccactgctcc
     8761 aaaaagcaac gcatcgtata tggggctact ggcagcgagg aaagatgcca aggaaaaggg
     8821 caatttgccg gtaccgctgc atttgcgtaa tgcggtgacg tctctgatga aggatctgga
     8881 ttatggcaaa aactaccgtt atgcccatga cgatccaagc ggtgccaaag cccaaaccca
     8941 tcttcctaaa gaattagaag gtcgcaaata ttatcaccac aagatccctt cctctccctc
     9001 gtctaatctg gatgaggaat gagatcataa ctcgtcctgg taattatgga gttctgaaaa
     9061 agagccggtt ttcggaccgg cagccgagcc acttttgttt cggcaaaagt ggccaaaacc
     9121 agtgacgccc cgtccagcct cattagatgg gacggacgca ggataggaag agcgggccaa
     9181 ctcgctacgc tcagacaagg cccgccgaat tcaaagagcg tccgacccga ttgctgggcg
     9241 gaaggcgtca gtccgcagcg aggccaccag gtagacaaaa ttgcttacca gtttatttgg
     9301 aagaacaaat taaaaaaggc tagtgatgat tttctgcaga agctgagaag aaaacggggt
     9361 gagtgctcaa agacagaacg gaagtttggc ctaagactca ggaaaaaaag ggcagcgttc
     9421 tcctttacaa aactatgcca gaccgttttg tttagcgtcg atatacggtg tatacgtgaa
     9481 atgctcgaag tgtactctga actcggactg actgcgagag ccttgccaga attcgacatc
     9541 cggattttgg ccacgaagaa ttagacacac gatttcgaga gctccacgtt cgatatcagg
     9601 accaaagtaa actgccttta gagctggcga tctgtagccg aatagtgttc caacctctgt
     9661 atggagccct cgccattctt tttcgtaggc ccatgcttca gatttcgtgc agaacaggtc
     9721 gatgagctga cctgggtcat tgtcgatgag gatagaatca atacggatct gaggcattct
     9781 ctcgacatat ttgactggaa gaagtttatt gaatggttcg tgctcagtac gaaattcaag
     9841 acacaatcct ttgtattgtc cgccataatg cgaccacatg agaagattat cgtttcgttc
     9901 cgaaaaacag gccaccccat tttttttgag gaatttttcg cgattttcat tcaaggctag
     9961 catggcacca ttgcggatat tttccttaag ttctggctgc gaaaatgtgg aaagctgcgt
    10021 tttgagttcg ggtggtacgt ccgagtcatc gagataatga tggagtaccc gctgcaactc
    10081 gtccggcgtt ggatcagaga tcgaagcggt aattgcacaa tcgtatgggt cattgaaatt
    10141 taagggtgag ctgaagtaga cggcttgagc tttgagattc aacagtgtgc gggttgtaaa
    10201 tgtttcgtat ttgaagatcg ttgagggtgg gagcatgtgg gcctatctaa atatagacgt
    10261 ccgaggtacg gtaagattat aaagaaaaaa tttgcccttt atgaaatatc ctaactgttg
    10321 taattaagtt cggtttaaag agtagttatt ttcgattagt tactaagttt ttatatttta
    10381 aaaaataaat ctgttggtaa ttacgagatt tgggattgtt gtttttcgag ttgggcgccg
    10441 aggtcggccc atttgtgggt gagacgatcg aggtcatggc cccattgttc gcgttcgtgg
    10501 tggagggaat tccagcggga gtagtcttgg taggtggctg ggtcggcgag ttccacgtct
    10561 cgagctttga tttgttcttc gagctgggca atgtcttttt ccactgtggt gacttgtttt
    10621 tccaaacggg cgatgctttt tgagaggtct cgtcgagacg gaccgtcagg aggggagacg
    10681 gttgtcctgg ctgtcccatt ggtggaatgc tcggtcgtgg gtttttcttt ttggggtttc
    10741 tcttttttct ttgatttttt cttgtcgact gtggcttccg gcggaatctg gtcttccagt
    10801 tctttggccc gtttccagca atagtattcg tagtcgccga tgtaatcgcg ggcttgtcca
    10861 tcgtctattt ccacgacgcg cgtggcgacc cgcatgagga atgtggggtc gtgcgagata
    10921 aacaccatgg ttccgggaaa ttggagcagt gcgtccgtta acatatccac ggatgtggga
    10981 tccaagtggt tggtcggctc atccaagagc aaggtgttgg ctggctcgac taacatgcgg
    11041 gctaaggcga ctcgattacg ttccccgccg ctcaaggctt tgatgggttt tttctgatca
    11101 tctccggaga ataaaaacgc gccggctaat cctcttaaaa aatttcgttc agtcatgggc
    11161 gcggcttctt caagagaatc caaaatggaa tgttcagatt tcagaatttc ggcctggtgc
    11221 tgagcaaagt agtggagggt gaccccgtga cccagggttc tggacccttt ttcgaattcc
    11281 aagactccgg ccaggatttt aagaagtgta gattttccag ccccgttttc cccgaccaag
    11341 gccacacgtt gtccccgttc gacggaaaaa tccaagccat catagatgat tttatcgcca
    11401 tagcgtttcc cgatatcccg tagttcaaat acgtcttttc cgctggccgg cgggtccggg
    11461 aatttaaatc gcaaccgttt cgtgtcccgc tgcagctcaa tgcgtttgac tttttcaagt
    11521 tgtttgattc gggactgcac ctggctggct ttgttggcct ggtatcggaa ccggtcgacg
    11581 aagttttgga cgcgtgcaat ttctttggat tgtcgattgg cggcggcctc ttcctgtgcg
    11641 tcccgctctg cacggagttt ttgaaaccgc gtataattgc cccggtattc ctgtaaggta
    11701 tggtttcgga cttcccaaat atgggtgacc atgttgtcta aaaatccaat gtcatggctg
    11761 atgatcaaca gggtgaaatt tgaagagagt aaaaatgatt ccagccaacg ctgcgtcggt
    11821 ttgtcgaggt ggttggtcgg ctcgtccagc atgaggacgt cgggggccga cagcaataag
    11881 tgggctaagg ccacccgcat gcgatagcca ccggagaggg tgtcaatctt gcgctcccaa
    11941 tccgactcct gaaatcctaa cccggcaaga atacgcttgg cttcgtgatc gggaaattga
    12001 ttccggtgtg tggcatcgag aatggtattt cccggcaaag actccagttc ctgtggcaga
    12061 tagccaaggc gaagaaatgg acgttgtcga atctttccgc tttctatctc ctcctcattg
    12121 agaatcatgc gcatcagtga ggttttcccc gacccattag gaccaacgag tcccacacgg
    12181 gtgttgggtt tgaggtgtgc cgaagcctca atgaacaggg gtttcgtggg gtagctcttg
    12241 gagatttgtt caatatgcag catgcggaaa caccatccaa tatgtgagat gaaaactgag
    12301 tcaaaacccc tttgtcatgg tcaaaaagac ggaaaggagg gatgaattgg tggagctggc
    12361 cgggattgaa ccggcgacct cgtgaatgcc attcacgcgc gctcccaact gcgctacagc
    12421 cccacaccga aaatgatgct atcacgcgac tacggttcgg tcaagaaaat cagaggccgg
    12481 aaaggcagaa ctccctcgtt gacaaattat gcgttgagac ctacgattca cccttgaagc
    12541 tctggctgac ccgctggccg agtttttttt tgtcctgtgc tcttgtcttg tgcgtgtgga
    12601 ggaagctatg gccgcattgg ttgttgttgg ctcccaatgg ggagatgaag gaaaaggaaa
    12661 aattgtggac cttctggcca aggatgcgga tatcattgtc cgctatcagg gaggttccaa
    12721 tgccggccat acggtcatta ccaataaagg cacctttatt tttcatttga ttccttcggg
    12781 tattctctat cgagggaagg tgtgcgcgtt ggggaatggg gtcgccattg atccggctgg
    12841 gttgatcgag gagttggacc gtttgaccac caaggggatt cccgttggaa aacgatttct
    12901 tattagtaat cgggcccatg tcatcatgcc ctatcataag gctattgata aggccgaaga
    12961 gcaggcgaaa ggggctcggc gaatcgggac gacgggccga ggcattgggc cggcgtatgt
    13021 cgataaaatg gcgcgagtcg gcattcgcat gggagatttg ctgaatccgg atgtgttgca
    13081 acaaaagatc cgggaaaatc tggtggaaat taatgcgttc ttagagcgca tttacaatgt
    13141 ggatggtttc accgttgaga aggttttcaa agattatcaa gcctatggtg agcgacttcg
    13201 tccgcatatt accgatgtcg cattggtcct ggaccgggcc tttgagcgag ggaagcgtgt
    13261 cttgtttgaa ggggcacaag ggacccattt agatgtcgat tttgggacgt atccctacgt
    13321 cacctcttca agttcctgct cgggtggggc gtgcaccggc agtggggtcg gtcccactac
    13381 gataacggga gtcttaggtg tcacgaaggc ctatacgact cgagtgggtg gaggtccgtt
    13441 tccgacagaa ttgactgatg aaatgggtca gcaactcatg cagcgtggga atgaatacgg
    13501 gtcgacgacg ggtcgggccc gccggtgtgg ctggttggac atggtgttat tgcgatatgc
    13561 cgttcgaatc aacggactca cctccttggc catcacaaag ttggatgtgc tggatggctg
    13621 caaggaattg aaaatttgta cgggataccg atatcaggga aaactccaca aagaaatgcc
    13681 aggtgaccta caggtgctgg ccggttgtga accggtgtat gaacgatgga gtgggtggac
    13741 aaccgacaca acgggtttga aagggtataa gggacttccg cctgctgcta aaaggtattt
    13801 agccagaatt gaggaattaa ccggatgtcg aatcgatatg atttcgaccg ggtctaaacg
    13861 tgaagatacc attgttgtga agaatccttt gattcggtcc cggaagggct catgatttac
    13921 acgcttcttt gtgttttttt ggctgtaagt ttgacaacaa tagaatccgt tgttagcatc
    13981 gcgacccggt gagccgctag ctcagttggt agagcatcgc ccttttaagg cgagggtcgt
    14041 gcgttcgagc cgcacgcggc tcaccataag ttaagtgcca tctccttctt gtgttttccc
    14101 ctcaataaac cagccagaat tctgggtaca tctcctattg gtagaatttc cttacccgtg
    14161 aaaatccttt agaaaaaata gtgcggcacc ctttatcaga aaaagggtgc cgaatgaagt
    14221 gcaacaaatt tagtccttgt gggctaagcg agccaggtta gcgttggctt gggacaacat
    14281 taaggcaaag gcggcaaaat cggtgtcggt caatggattg ccggtagaat atctatctac
    14341 caggatgact ccaataggcc tgttgggcgc gtataaggtt ccggcgaacc cgggttggtt
    14401 gccccagtat tttagaaagg cggttggcat gcctccggat tccccttctt ggaccaatga
    14461 agaatattga atagggtgaa aactatggag cagtcgatcc cagaagtttc catgggaaag
    14521 agggcaatgg aacacgggtt ggatggtttc cacatgcgga ccataaccga tcttggcctg
    14581 gagcatatct tttcctggta cgaccaatgc gagccagacc cgctcaaatc catacgaagc
    14641 atgaagggtt cgggtgacaa aggtaagcag caaggagggt tcggatattt tcatggactg
    14701 aatggtgaaa tccagcaatg gcctatcgga ggccggagag ggctggaggg aaggggatgc
    14761 ttgctgagcc gatgttgcta attctctgga aagaacccct tcggggtcta gaacctgatg
    14821 gatcgtttga gtgagccgat gtagctgtgg attgtgaaga aatagttcat cagcctcgga
    14881 tgcgggcaga aagtgctgcc ggtcaatgtt gatggccgag gcgatttctg aggcctggct
    14941 aaaagctttt gacacggtgt gttccaattg gatcatggag aggccgaatg ggggaagaaa
    15001 ctgttcgatc aggccttgga gaacctctcc ctgtccggca gccaatgggt tcaggaggca
    15061 ataacttaat tcattcgcgg ctcgaactat tccttccatg atctgttgat ctgcttcggg
    15121 gcgggatttg ggccagacgg gctttttctc caatagttga accaggctgt ccggcagatt
    15181 ccaatgcttg gccatggcgg ctgcgatgac atgcagtggt cttcccaaaa gagttagttc
    15241 ggcttttggg atcttggctg gatcctcccg gcgtatgact gctagttgtt cggcgacgtc
    15301 gggtcgacag agagcgagga tgaggtctcc cagtgaatag agcatggcat tggtaaataa
    15361 cgatccc
//
LOCUS       DATIAL010000043         5466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_115758, whole genome shotgun sequence.
ACCESSION   DATIAL010000043 DATIAL010000000
VERSION     DATIAL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115758"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VLA60_01410"
     CDS             <1..722
                     /locus_tag="VLA60_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HSF08037.1"
                     /translation="LLYGTKAKLHNGLESWGLLLVTGPKSPQVLKDGLNLEVGSLQVL
                     DTITFVLGDTQGFLARTEETGGQDYELFIPVEALPSLWSHLLNTGKELGLLPVGREAL
                     ETSRIEGGLARLGPDLNEKIVPPEANLEGIAFSLSKGCYPGQEVVARMDTYGSVKRRI
                     VGLVLEAEIPQLPEPGAKLFSGTREVGWVSSSTHSPLVKKPIALGFPLRDFTQPRTKL
                     EIEIRDQRFPASVCALPFTAS"
     gene            complement(768..1244)
                     /locus_tag="VLA60_01415"
     CDS             complement(768..1244)
                     /locus_tag="VLA60_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08038.1"
                     /translation="MLTATDVFEQTREMALRATSQEERALQIDYPALSTKIEQALTGR
                     KVNLLYVNEFLPEGYEDQGRFNVMVMTSGQVLFDMVIGDSYFRYDVFSCCDLDKIQLI
                     DGTWDNKEKRTEESFLSLRLMHGDEAHILLALNDAQRPAVLSFAEKISHGRHPEKA"
     gene            1483..1704
                     /locus_tag="VLA60_01420"
     CDS             1483..1704
                     /locus_tag="VLA60_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08039.1"
                     /translation="MDADTCLIGGCTEPLYAPAGTQSLCKEHFLQFVTWRRKKGGLGM
                     FKKYCALNMEERDAIVEEWAKTTFSTSAS"
     gene            complement(1854..2246)
                     /locus_tag="VLA60_01425"
     CDS             complement(1854..2246)
                     /locus_tag="VLA60_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSF08040.1"
                     /translation="MLKERIEEVTQMNELFYRGFEQLDVALMDSIWAHQEYVTCIHPG
                     WSIRVGWPAVRDSWVVIFNNTFSMKFELTEVQVQVAADVAWVICTENITSRVGENEQN
                     SQVVSTNLFERIGDEWKIIHHHGSPLME"
     gene            complement(2284..3207)
                     /locus_tag="VLA60_01430"
     CDS             complement(2284..3207)
                     /locus_tag="VLA60_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSF08041.1"
                     /translation="MKNVSPGSSAFSAYAALTTAAVVWGGSVVAQKVALGPFSPVEAS
                     VFRGLGALLILIPLWLWKEGAVSFSRRDWRNFFFLGIGVLGNHLFVLFGLQFIGAGAA
                     GIIIGSSPAITAFLSSLILKDIPLRVIWLGCLVSFLGVLSISGRGAVDGLGSNPLLGG
                     SLVVCALVSWALYTIGCRKTMERFSPLTVTWTTLLLSLIFEIPLLSLNHKVLVAGVES
                     VPVSGWLALFYVMVFATALGQQAWLLGVKGIGPSRAGIFGNLIPVSALCFSVMILGEP
                     VGMREISGIGLILIGVWLVDRQSCAVLVKVP"
     gene            3736..4083
                     /locus_tag="VLA60_01435"
     CDS             3736..4083
                     /locus_tag="VLA60_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSF08042.1"
                     /translation="MSLLITEECINCGACLPECPNEAIFETRSAAEEKGNKVGEGQGE
                     GDTVYVITYERCTECVGHFDEPQCAAVCPVDDCCIPDPEIPETTDTLLDKAKQLNPDK
                     EIDPAKVWAGVRN"
     gene            complement(4189..>5466)
                     /locus_tag="VLA60_01440"
     CDS             complement(4189..>5466)
                     /locus_tag="VLA60_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08043.1"
                     /translation="TRADEDEALRQLLDALNTIDQERQDMLQSLNDTRMNFQALKSQW
                     DYEQANVERIEREEEHRKTRIRQIDGQLEHLFLQSRKSQEERLTNESLVEELDIQKEA
                     IAGTLLELQNRHAGCLEEVKRLDGLIGKARETLSHIFKSRVPIEGRLAEVRAKFHAVE
                     ETLTMTYEISTDDLKNHPDSGQIAEGLGGSEQASEEKTGQWREQLQGIRKKLERIGPI
                     NLAAIEEHAQLEERYQFLLAQEEDLAGSIQSLQEIIQRLNQTTNKLFAETFKDLQVKF
                     SEVFSALFAGGRAELILVEDTQEGQESEGPNLEPGVEIVAQPPGKRLKNLNMLSGGEK
                     TMTVMALLFASFLIRPSPFCVLDEVDAPLDETNVVRFGQFLRQMADRSQFIVITHNKR
                     TMETANSLFGVTMEDPGVSKLIAVRLHELEEVS"
BASE COUNT         1501 a         1442 c         1157 g         1366 t
ORIGIN      
        1 ttctgctcta tgggacaaaa gccaaacttc acaatggctt ggagagttgg ggactgctcc
       61 tggttaccgg gccgaagagt ccgcaggtgc ttaaggatgg cttgaatttg gaagtaggat
      121 cgctccaggt cttagacacc atcaccttcg tattaggtga tacacaagga tttcttgctc
      181 gcacggaaga aacgggggga caagactacg aattgtttat accggtcgaa gcccttcctt
      241 cgctctggtc tcatctcttg aatacgggaa aagagctagg cctgctcccg gtcggaaggg
      301 aggcccttga gacatccagg attgaaggtg gattggctcg ccttgggcca gacttaaatg
      361 aaaaaatcgt tccgccagaa gccaaccttg agggaattgc ctttagttta tcgaagggat
      421 gttatccggg gcaggaagtc gtcgccagaa tggataccta cggatcggtg aaacggcgaa
      481 ttgtcggctt agttcttgaa gcggaaatcc cacaacttcc ggaacctggc gcaaaacttt
      541 tcagtggaac tcgggaagtg gggtgggtgt ccagctccac ccactccccg cttgtcaaaa
      601 aaccgattgc ccttggattt ccattacgag atttcaccca accccggaca aaactggaaa
      661 tcgagatcag agaccagcgc ttcccagcct ctgtctgcgc cttaccgttt acggcctctt
      721 aatcagcttc gagattccaa aagaattctt ccgcactctg aaagggatca ggctttttcc
      781 gggtggcggc catgagagat tttttccgca aaagacaata ccgctggacg ttgtgcgtcg
      841 ttaagcgcaa gaagaatatg cgcctcatcg ccatgcatca atcgtagact aagaaatgat
      901 tcttccgtgc gtttttcctt attatcccaa gtcccatcaa tcaattgaat tttatccaaa
      961 tcgcaacaag aaaacacgtc ataccgaaag tacgaatccc cgataaccat atcgaaaagt
     1021 acctggccag acgtcataac catgacgtta aaacgccctt gatcttcata gccttccggc
     1081 aaaaattcat tgacatacag aaggttaacc ttccgacctg tcaatgcctg ttcaatcttc
     1141 gtgctcagag ccggataatc gatttgaaga gcccgctctt cctggctcgt agcgcgcaac
     1201 gccatttctc ttgtttgttc gaacacatct gtagcagtca acacagaaca cgctcctttc
     1261 ttccacgaca ctattaaatt atgtcgaatt ggtttgtcta aacagggtgc ctacgatacc
     1321 cgccatttga cagagaaagc aagggaacca gacccctcta ttaatggctt ccacgtataa
     1381 ttatttttta tcatggcgtg aattgattgc cttctcagct cgcagttggg catacttcga
     1441 atgaaaaatg ttgccggagg aacctttaag gagaaccagc ttatggatgc cgatacatgt
     1501 ctaattgggg gatgcaccga acccctctac gcccctgccg gaacccaatc gttatgcaag
     1561 gaacactttc tacaattcgt cacgtggcgc agaaaaaaag gcggactggg tatgtttaaa
     1621 aaatattgcg cgttgaacat ggaagaacga gacgcgattg ttgaagaatg ggctaaaacg
     1681 acattcagca cctctgcctc ttgatcagtc ctctcttgcc cttcacacgc gttgaatcat
     1741 gatccggacc tcatgcctcc tttccgattc caaaagtgag gagatctcta ctcctaccta
     1801 agtaacagaa gaaataccca cacgtcgcca agtacccaat ggacgattaa accttattcc
     1861 atgagcggtg atccgtggtg atgaatgatc ttccactcgt ctccgatccg ctcgaataaa
     1921 ttggtcgata ccacctggct gttctgttcg ttctctccca cccgactggt gatgttttcc
     1981 gtacaaataa cccaggcaac atccgcggct acttgaacct gaacttccgt cagttcaaat
     2041 ttcattgaaa acgtattatt aaaaatgact acccaggaat ccctaaccgc cggccacccg
     2101 acccggatgc tccaacctgg atgaatgcag gttacatact cttgatgagc ccaaatagaa
     2161 tccatcagcg caacatccag ctgttcaaac ccacggtaaa acaactcatt catctgcgtc
     2221 acttcctcga tccgttcctt cagcattgtg cgctctcttt cttcattttc gcccatttcc
     2281 taatcagggg accttcacta acacggcaca ggattgtcga tcaaccaacc agaccccaat
     2341 cagaatcaat cctatcccac ttatttctct catgccaacc ggttcaccca aaatcatgac
     2401 cgaaaaacac aacgcagaaa ccggaataag attcccaaaa attcccgctc tggagggacc
     2461 gattcctttg acgccaagca accaggcctg ttgacccaat gccgtggcaa acaccatcac
     2521 gtaaaaaagt gccagccagc cggataccgg aaccgactct acccctgcaa ccaggacttt
     2581 gtgatttagg ctcaacaaag ggatttcaaa gatcaaagag agtagaagag tcgtccacgt
     2641 cacagtcagc ggagaaaacc gttccatggt ttttcgacag ccaatcgtat atagagccca
     2701 actgactaac gcacagacca ccagactccc tccgagtaaa ggattggaac ccaatccatc
     2761 caccgccccg cgcccggaaa tactcaatac tcctaaaaac gagacaagac aaccaagcca
     2821 aatcacacgc aggggaatat ccttaaggat gagtgaggac aagaatgcgg taatggccgg
     2881 gcttgaacct ataatgatgc cagctgcccc tgccccaata aattgcaggc caaagagaac
     2941 gaacaagtga ttgccaagga ccccgattcc taggaaaaaa aagtttcgcc aatctctccg
     3001 tgaaaaagaa accgcccctt ccttccagag ccatagagga ataagtatca gtaaggctcc
     3061 taatcctcta aaaacagacg cctctacagg cgagaatgga cctaaagcga ctttttgtgc
     3121 aactaccgac cctccccaga ccaccgcagc agtcgtaaga gcagcatagg cggaaaacgc
     3181 tgaggatccc gggctaacat ttttcatggg gaggcaagtg agaaaaatac ggaaggtgct
     3241 gtaaccctca atcgccctat caggcaagac aacccctacc tcgaacgaac tcttggatga
     3301 gcctaccagg agtatatcca cacatacaaa atctttcgct ctacattctt actcaaaaat
     3361 gactcctgca aatgcgacgt gtcctctcca tcagttttga gaaggcatcg ctgcctataa
     3421 caatcgtcca taggttcgtc aagatcagac caaattatag tcctccaaca aatatataaa
     3481 tgtactcttg attggtatga ttacgggctc cagcaatttc ttagaaaagc ttctgaatat
     3541 ccctcattta atcgcgggcc cgaatggctt atcggatagg tcttctgtcc ggttgacgca
     3601 aagaaattta caccgttaaa atcgccaaag agttgatact gtaccgccct catgccaaaa
     3661 cattttcaat aagaatcgta ggcgttggct tcctacaatt gacatgacgg tttatctgtc
     3721 ccctaaggag gaattatgtc gttgctaatc acagaagagt gcattaattg tggtgcctgt
     3781 ctcccggaat gtcccaacga ggcaattttt gagacacgca gtgccgcgga ggaaaagggc
     3841 aataaggtcg gtgaaggtca gggtgaaggg gacacggtgt acgtgatcac ctatgagcgc
     3901 tgcacagagt gcgtcgggca ttttgatgag cctcaatgtg cggctgtttg tcctgtcgat
     3961 gattgctgca tacctgatcc agaaatccct gagacaacgg atacactact ggataaggcc
     4021 aaacagctga atcctgacaa agaaatcgac ccagcgaaag tttgggccgg cgttcgaaat
     4081 taaccaaccc caaaaacatg gctctgtaaa aaaagcccct tccgactttt caaggaaggg
     4141 gctttttctt ttggactcca actaatgatg gatccccatg gtgtaccctc agcttacctc
     4201 ttctaattca tggagccgga cagcaatcaa tttcgaaacg ccaggatcct ccattgtcac
     4261 accaaaaagg gagttggcag tctccatggt gcgtttattg tgtgtgatca caataaattg
     4321 cgatcggtcc gccatttggc gcaaaaattg accaaacctg actacgttcg tctcatctaa
     4381 tggcgcatcc acctcatcca acacacaaaa tggagaaggt cgaatgagaa aactcgcaaa
     4441 gagaagggcc atgaccgtca tggtcttttc ccctccggaa agcatgttga gattttttaa
     4501 gcgcttccct ggtggttggg cgacaatttc cacacctggc tcaagattgg gcccttctga
     4561 ttcctgtcct tcctgagtat cttcgactaa aatcaattca gcgcgtcctc cagcaaacaa
     4621 ggctgaaaag acttcgctaa atttcacttg aagatcttta aaggtttcgg caaacagctt
     4681 attggtagtt tgattcaaac gctggataat ttcctgaagg gattggattg aaccggctaa
     4741 atcttcttct tgcgctaata aaaattgata gcgttcttct aattgagcat gttcctcaat
     4801 agcagccaaa ttaattggac cgatacgctc taattttttc ctgataccct gtaattgctc
     4861 tctccattgg ccggtttttt cctccgaagc ctgttcactt cctcccagac cttccgcgat
     4921 ctgtccggaa tccggatgat ttttgaggtc atccgtcgaa atttcgtatg tcatggtcag
     4981 cgtttcttcc acagcatgaa atttcgcccg aacttccgct aaacgcccct cgatcggcac
     5041 acgggactta aagatgtggc tcaatgtttc acgagccttc ccaatcagac catccaaccg
     5101 tttaacttcc tccagacatc cagcatgacg gttctgaagc tccaataatg ttccggcaat
     5161 ggcctctttc tgaatgtcta attcttccac caaactctca ttcgtaaggc gttcttcctg
     5221 actctttcga ctttggagaa agaggtgctc caattgtcca tcaatttgtc gaattcgggt
     5281 tttccgatgc tcttcttccc gttcaattcg ttcaacatta gcctgctcat aatcccattg
     5341 ggatttcaaa gcctgaaaat tcattcgtgt gtcattgagg ctttgcaaca tatcctgtcg
     5401 ctcttggtct attgtattaa gagcatccaa caactgacga agtgcctcat cctcatcggc
     5461 tcgtgt
//
LOCUS       DATIAL010000044         8287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1083560, whole genome shotgun sequence.
ACCESSION   DATIAL010000044 DATIAL010000000
VERSION     DATIAL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083560"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2126)
                     /locus_tag="VLA60_01445"
     CDS             complement(<1..2126)
                     /locus_tag="VLA60_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HSF08044.1"
                     /translation="MPLLIPEKKSSLGPDLAKKLGQDKVRWDWATLKAYSVDASIYKI
                     PPQVVVLPETEEDIHAVMEYAVKVGVPLTPRAAGTNLTGSAIGAGIIVDISRMNRLLE
                     VNRDEQWARVQPGLVLTEFNKQLAPMGLMYGPDPSSGDMCKLGGMFGNNSAGPHTLRY
                     GSVKDNVHTLRIRLLTEGWLDAQSVHIGGPAHTSLLKGFPSLKAVWTMIQEDQALIRA
                     RRPKVSKNSSGYNVFDLVDGLERGVFDVQKLFIGSEGTLGIISEATVKLVKRPDATVT
                     GLIHFRHLEGMGESVPHLLELSPNALEVMDGNSLDLIGRNRYGIPSDAAATLLIEFDE
                     GGGADRRHRLMEMCKRFPLTGEVRLASDPAQQGELWKARKALFPMLYRFDPQKKPINF
                     VDDVVVPASRTGELIRYLEEYFGGRSVPVAIFGHVGNGNAHIVPLLDLHNQHDVDAMV
                     EAHREIHQTVLDRFDGSICGEHGDGRIRAETVRLMYGEEVYQIFVRVKQAFDPQGMMN
                     PGVKISDTSFTEHIDVERMGKPCATCAKCNSVCPVYDVFQSEDMSARGWFEIVTASDY
                     SYLQSERVVDACLNCKSCRTICPAGVDVSELILQRRAEHPNELAGWVFALHARQWIFH
                     PLLKLMAWTQSFWDRPFIRDMLEKLTRPLLRNLAPTAKIPRDMVLPTFAKRHLRDRYP
                     ELCHSEHLRQASIAYFHGCAANYFEDG"
     gene            complement(2233..2997)
                     /gene="scpB"
                     /locus_tag="VLA60_01450"
     CDS             complement(2233..2997)
                     /gene="scpB"
                     /locus_tag="VLA60_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HSF08045.1"
                     /translation="MNALTNGHQDSDMLDAEVTEDRTLSEVSEVSDEGRDAEEVTDSE
                     IVDQEPALDVGELKAAFEALLFVSHDPLSLEKLALVLEGVPKTFVKSAMQELQAEYEA
                     SGRGLQILEVAGGYVMVTRPEQSLYIKRLTSKAKPAAKVSRSALEALAIVSYKQPITR
                     ADIEKIRGVETSGVLRTLLDQKLIRIVGRQDIPGRPILYGTGKQFLQRFGLRDLRDLP
                     PLKELKELGSPEQFPMELPFAPEEQEGEARVTDLSA"
     gene            complement(3119..3919)
                     /locus_tag="VLA60_01455"
     CDS             complement(3119..3919)
                     /locus_tag="VLA60_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HSF08046.1"
                     /translation="MVVSDLEDDDAYQVKLEAFTGPMDLLLHLIRKHQINIYDIPVAL
                     IAQQYLEYLSMMKTLNLSLAGEFLVMAATLLYIKSRMLLPKEEKPETEDEEGLDPRAE
                     LVRQLVEYERFKEAAGSLVIRERLWRESFTRDPLPLPKESAVEEDMATEDLQLFDLLS
                     AFQDVLDRAPTNEIVELSRETWTVQDRIQVILERLEAESTVPFEGLFEHHWSKPLVIT
                     TFLALLELVRMNLVRLFQGEWLGPIQVTRRFVPAGKADSTGSFPEDSL"
     gene            complement(4043..5134)
                     /locus_tag="VLA60_01460"
     CDS             complement(4043..5134)
                     /locus_tag="VLA60_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF08047.1"
                     /translation="MKLRGAKYSLQLGLLLALLGLSSINAGACRLLPIPPGSENVFWV
                     HVEGPCLPGEETALAVKGADLLEALVQGKRIDLEHVLVVDQVMLDLLPQQALSEYPAI
                     PPSVRKPLKEKGITAVRVIPEAISIRHSRFEKVLATNLVEGALLILGTVDFTGTVFQQ
                     SIDLSKTVFVGPVRFSHARVDFEGFFIGSQFAQGVDFSQVIFGTHSRFHQAQFRDRVT
                     FADAHFTGVAEFLEVEFQQAADFSRTAFASGTGFSGSVFKGPADFSGISAVHEIYFRF
                     TEFRESVTFAQAQFHTVVDFSNARFGGAKDFSGAEFRTMPELTGSNLPLDVVPVQNGR
                     RLYGQVGIFLGLVILVLCYLWLSKGKKTA"
     gene            complement(5146..5349)
                     /locus_tag="VLA60_01465"
     CDS             complement(5146..5349)
                     /locus_tag="VLA60_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08048.1"
                     /translation="MNRTCTIVQDLARFSDPFVQQLRSLQTIERFDSVRRNLVTIIYP
                     HQSLCVCARVEAVGFPVCHTMLM"
     gene            complement(5362..6126)
                     /locus_tag="VLA60_01470"
     CDS             complement(5362..6126)
                     /locus_tag="VLA60_01470"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSF08049.1"
                     /translation="MSEERGLPRIGDHAPDFSAVTTQQTDFNFSVWQEQHWVVLFSHP
                     ADFTPVCTTELMAFAQASEQFRQRGVKLIGLSVDSIHAHLAWLRSIHDKMGATIPFPM
                     IADVDMRVAKLYGMIHANASSTATVRAVFIIDPKRVIRALLYYPLNAGRNVDEILRLV
                     TALQTTDRYACATPANWREGEKVVVPPPKTVQEVDQALAKTEYEHRDFYLALKNVTPV
                     VTPKPAEVRETQPAEAEAPAPAKPEPAQPAENTSST"
     gene            complement(6280..7143)
                     /locus_tag="VLA60_01475"
     CDS             complement(6280..7143)
                     /locus_tag="VLA60_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08050.1"
                     /translation="MAAMAKPKSKAKPKQKPTGKRTTHAKAQPKTKAKPKKSVKPPRS
                     PSTKAKSKNTKVGSKARTKGPVKTQAKGNTKARVKGTSKAVAQAQPRSKTTPKKAVKG
                     KEKKLTTSDNVSKTTNAAKTGGNRTKSAPTTKSVKKAAPSEAKKKPGRPKKESAVEKG
                     LDAKKKKNAFPVDVDAEEILSQESDTDIEDTSLPDDIDSELDIEVETDADITLDSLTT
                     ELDDVTDPLDVDDLADDFDDDEFSEDSTDDADEDLDLTEDLSEELGTDKFFRDTESNH
                     EDDEEEIPRSW"
     gene            7323..>8287
                     /locus_tag="VLA60_01480"
     CDS             7323..>8287
                     /locus_tag="VLA60_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HSF08051.1"
                     /translation="MEIITTHLEADFDGLASMVAAQKLYPEAHLVLPAGVQSRERAFL
                     AEHPLSFTALSSLNFPDITRLILVDAQHQDRLGLLEKVLKNQHISVHIYDHHPLEATN
                     PLLPMADYCKVDSVGATITLLCEELQTRGLTWTPEEATFFAIALYEETGQFGYPNTTP
                     RDFQMGGVLVQTGADLNQVTKYIARHWTPPQLELFHALLQSAQTLNLGHRRILLTTLT
                     WPDYVQDMAPVVQQLAQLHGADAIIAAVAMDGKVQIIGRSRHPDMDMNQITKAFGGGG
                     HTMAAAASIKGLTIVEVEQKIHALLQEQAKTWLPIKRLMTTPVRTV"
BASE COUNT         2088 a         2367 c         1903 g         1929 t
ORIGIN      
        1 ccatcctcaa aatagttggc cgcacaccca tgaaaatacg cgattgaggc ctgccggaga
       61 tgctcagaat gacacagttc aggataccga tcacgcaaat gccgtttagc aaacgtcggc
      121 agaaccatat ctcgtggaat ctttgccgtg ggagccaaat ttcgcaataa cgggcgggtc
      181 agcttttcca acatgtctcg aataaatgga cgatcccaaa aagactgggt ccaagccatg
      241 agcttcagca ggggatgaaa aatccattga cgggcatgga gggcaaagac ccaaccggcc
      301 agttcgttgg gatgctccgc ccgccgttga agaatcaatt cggacacatc gaccccggcc
      361 ggacaaatgg tccggcagga cttgcaattc aaacaggcgt cgaccacccg ttcggattga
      421 aggtaggagt aatctgaagc ggtcacaatc tcgaaccacc cgcgtgcact catatcttca
      481 gactgaaaca catcatacac cggacacacg gaattacatt ttgcacaagt tgcacaaggt
      541 ttgcccatgc gctcaacatc aatatgttcc gtaaacgagg tatcactgat tttcactcca
      601 ggattcatca tcccctgtgg atcgaacgcc tgcttgactc gaacaaaaat ttgatacacc
      661 tcttccccat acatcaagcg aacggtctcg gcgcgaatac gcccgtctcc atgctctcca
      721 caaattgacc cgtcaaaccg gtctaaaacc gtctgatgaa tctcccggtg ggcctcgacc
      781 atggcatcaa catcatgttg attgtgcaaa tcaagcagcg gcacaatatg ggcattgcca
      841 tttcccacat gtccaaagat ggcgaccggc accgatcgtc ctccaaagta ctcttccaaa
      901 taccggatca attctccggt gcgtgaggcc ggcacgacca catcatcgac aaaattgatg
      961 ggcttttttt gaggatcaaa tcgatagagc atcggaaaca acgccttgcg ggctttccac
     1021 aattcccctt gctgagctgg atccgacgcc agcctcacct ctccggtgag cggaaacctt
     1081 ttgcacatct ccatcaaacg atgacgacga tctgcgcctc cgccttcatc aaattcaatg
     1141 agcagcgtcg ccgctgcatc tgatggaatg ccatagcgat ttcgcccgat caaatccaac
     1201 gaatttccat ccattacctc caaggcatta gggctcaact ccaataagtg gggaaccgat
     1261 tcgcccatac cctccagatg tcgaaaatgg atgagacccg tcacggtggc atcaggtcgt
     1321 ttaaccaact tgaccgttgc ttcacttata atgccaagcg ttccctcgct cccgataaat
     1381 aatttttgaa cgtcaaatac gccccgttcc aatccatcga ccaaatcaaa gacgttatag
     1441 ccggaactgt tcttactcac cttcggacgc ctggcacgga tcaacgcttg atcctcttga
     1501 atcatggtcc acaccgcctt gagggatgga aaccctttca ataaggaggt gtgagccggc
     1561 cctccaatat gaacggattg agcgtcaagc caaccctccg taagcagccg aatccggaga
     1621 gtatgaacgt tgtccttgac cgatccatag cgaagcgtat gcggaccagc cgaattgttg
     1681 ccgaacatgc ctcctaactt gcacatatcc ccgcttgaag gatccggccc atacatcaac
     1741 cccatagggg ccaactgctt attgaattcg gttaacacca ggcctggttg cactcgcgcc
     1801 cactgctcgt ctcgattcac ttccagcaat cgattcattc gggagatatc cacgataatg
     1861 ccggctccga tggctgatcc ggtcagattc gttccggcgg cccgtggagt cagaggcact
     1921 ccaaccttga cggcatactc catcacggca tgaatgtctt cttctgtttc aggaagaaca
     1981 acaacctggg gaggaatttt gtagatactg gcatcgacgg aataggcttt cagcgtggcc
     2041 caatcccacc gaactttatc ctggcccaat tttttggcta aatcgggccc caaagaagat
     2101 ttcttttcag gtatcaacaa cggcatagga actcattctg ctccctacga ggatggcgca
     2161 caacccttgt cagagagaat acaggaagga aaacaagcag accacagcag gacgcgtggc
     2221 cccacggtcg atttaagcag aaagatcggt aaccctggcc tctccctcct gttcctcagg
     2281 ggcaaaggga agctccatag ggaattgttc cgggctaccc aactctttga gttctttcag
     2341 cggaggcaaa tctcgtaagt cccgcaaacc aaaacgttga agaaactgct tgccggttcc
     2401 atacaaaatg ggtcggcctg gaatatcctg ccgcccgacg attcgaatta acttctgatc
     2461 aagaagggta cgcaacaccc cggaggtttc tacacctcga attttttcaa tatctgctct
     2521 ggtaatgggc tgtttataac tcacaattgc cagggcttcc agtgccgacc gggaaacctt
     2581 ggctgccggt ttggccttgc tggtcaatcg cttgatatag agactctgct cgggtcgcgt
     2641 taccatgaca tatccgccgg ccacttccaa aatttgcaag ccacggcccg acgcctcata
     2701 ctccgcttga agctcctgca ttgcgctttt aacaaacgtt ttaggcaccc cttcgagaac
     2761 cagagccagc ttctcaagac tcaaaggatc atgcgaaaca aagagtaggg cctcaaacgc
     2821 ggctttgagt tcaccaacgt ccaacgccgg ctcctggtct acgatctcgg aatccgtcac
     2881 ctcttctgcg tcccggcctt catctgagac ctctgagacc tcacttagtg tacggtcttc
     2941 cgtcacctcg gcatcgagca tgtccgaatc ttggtgtcca ttggtgagtg cgttcatgat
     3001 tattgagtcc tcctctgcgg actgaacccg atcattccac gttgtctcac gcctaattgt
     3061 ctcaggccat tcctacatcc ccccatgcgt cctccgatca atacccggct catacgtgtc
     3121 acaatgaatc ctcaggaaaa cttccagtag aatcagcctt cccggctggg acaaaacgcc
     3181 tggtcacctg gatgggaccc aaccattccc cttgaaacaa tcgaaccagg ttcatcctga
     3241 ccagttccaa cagggccaga aatgtggtaa tgaccaatgg cttggaccaa tgatgttcaa
     3301 ataacccctc aaaaggaacc gttgactccg cttccaaccg ctccaagatg acttgaatac
     3361 ggtcctgaac tgtccaggtt tccctggaca attccacgat ttcgttagtt ggagctcgat
     3421 ccagaacgtc ctggaatgca ctcagaagat caaataattg gagatcctcc gtagccatat
     3481 cctcctcgac cgctgactct ttgggcaagg ggagaggatc cctggtaaag gactcccgcc
     3541 aaagacgttc gcgaataacc aggctcccgg ctgcttcctt gaaacgctca tattcgacca
     3601 attgccggac caattccgca cgaggatcca gaccttcctc atcttccgtc tccggttttt
     3661 cctctttggg aaggagcatc cgggatttaa tataaagcag cgtggctgcc atgaccagaa
     3721 attccccggc cagcgacagg ttcaacgtct tcatcatgga caaatattcc aaatattgct
     3781 gagcaatcag cgccaccgga atatcataaa tgttgatttg gtgtttccga atcaagtgga
     3841 gaagcaaatc catggggccg gtaaaggcct caagtttgac ctgatacgca tcgtcatctt
     3901 caaggtcgga aacaaccacg gaggggacgg attccaatca agtgcctttc gactgggttc
     3961 cacagtgcag atatttgaat tggtatacca tcttttgtgt aaagccgcaa tatcctaacc
     4021 aaatatagtg gttaagacct ctctaagccg tcttcttccc tttggaaagc cagaggtagc
     4081 agagcaccaa aataaccagc cccaaaaaga tcccgacctg gccatatagc ctgcgaccat
     4141 tttgaaccgg aaccacatca agcgggaggt tactcccggt taattccggc atggtgcgga
     4201 actcggctcc ggaaaaatct ttggctccac caaacctggc gttcgaaaaa tcgaccaccg
     4261 tatgaaattg cgcttgggca aacgtcaccg actctcgaaa ttcggtaaac cggaaataga
     4321 tttcatgaac ggccgagatg cctgaaaaat cagccgggcc tttaaacacg gaccctgaaa
     4381 agcctgtccc actggcaaaa gccgttcggg agaaatctgc cgcctgctga aactcgactt
     4441 ccaaaaactc tgcaactccg gtgaaatgag cgtcggcaaa ggtcacccga tcacgaaatt
     4501 gagcctgatg aaaccgggaa tgcgttccaa aaatgacctg agagaaatcc acgccttggg
     4561 cgaattgaga tccaataaaa aacccctcaa aatcaacccg cgcgtgtgaa aatcgtaccg
     4621 ggcccacaaa tacggtctta gaaagatcaa tagattgctg aaagacagtt cccgtgaaat
     4681 ctaccgtccc caatatcaag agagcccctt caaccagatt ggtggcaagc actttttcaa
     4741 atcgagaatg tcttatggaa attgcctctg gaatgacgcg aaccgcagtg atacctttct
     4801 ccttcagggg ttttctcact gacgggggaa tggcgggata ttccgaaagg gcctgttggg
     4861 gcagaagatc aagcatcacc tgatcaacca ccaacacgtg ttcaaggtca atccgcttac
     4921 cctgcacaag cgcttccagc agatcagccc ccttcacagc caaggctgtc tcttctccag
     4981 gcaaacaggg tccttcaaca tgcacccaga aaacgttttc gcttcctgga ggtatcggca
     5041 ggagacggca agcccctgca ttgatagacg acagccccag cagggcaagc aacaagccca
     5101 actgtagaga atattttgct ccccggagtt tcatctatgc ccacatcaca tgagcatggt
     5161 atggcaaaca gggaacccaa ctgcctccac ccgggcacat acgcaaagag attgatgagg
     5221 atagatgatc gtcacgaggt tgcgtcggac tgagtcaaat cgttcgatcg tttggagcga
     5281 gcggagttgt tgaacaaagg gatcagagaa tctggccaaa tcctgcacaa tcgtacacgt
     5341 ccggttcatc tcggacagcc tctaggtgga agaggtgttc tccgcaggtt gagccggttc
     5401 gggtttagca ggcgcgggag cctcggcttc cgcaggttgg gtctccctca cttccgcggg
     5461 ctttggggtc acaaccggag tcacgttctt gagcgctaaa taaaaatccc gatgttcata
     5521 ttcggtcttt gccaacgctt gatcgacttc ctgaacggtc ttgggcggtg gcaccaccac
     5581 cttttcccct tcccgccaat tagcaggcgt cgcacaggca tagcggtcag tcgtttgcag
     5641 agcagtgacc aaccgaagga tttcatccac attgcgtccg gcattgagtg ggtaatacag
     5701 caacgcccga atcacccgct tcgggtcaat aataaacaca gcacgcacag tcgcggtgga
     5761 actcgcattg gcatgaatca tgccgtacag cttggcgaca cgcatatcaa catcggcaat
     5821 cataggaaat ggaatcgtgg cacccatttt atcgtgaata ctgcgaagcc aggctaaatg
     5881 ggcatgaata ctatccacac tcaacccaat gagcttcacc ccccgctgac gaaattgctc
     5941 actggcttgg gcaaaggcca tcaattccgt cgtacacacc ggagtaaaat ccgccgggtg
     6001 agaaaacaat accacccaat gctgttcctg ccatacgcta aaattaaaat cggtctgttg
     6061 cgtcgtcacc gcggaaaagt ccggtgcatg atccccgatg cgaggcaacc ctcgttcttc
     6121 cgacatgaaa cctccttcag ttggcaagac tatccccctt cgcctcaggc gaagggagca
     6181 aacccggctt gtctataaaa ccgggaatac gaacaatact atccagttgg cgaagagaat
     6241 ggccctggca cagcctttgg ggcccatgaa aggccaaaat caccaggagc gaggaatttc
     6301 ctcttcgtca tcctcatgat tggattcggt atcccgaaaa aacttatccg tgcccaattc
     6361 ttctgataag tcttccgtca aatccaaatc ctcatcggca tcgtcggtgg agtcctcgga
     6421 aaactcatca tcatcaaaat catccgcaag atcatccaca tccaggggat cagtgacatc
     6481 atcgagttca gtggtcaatg aatccagagt gatatcggca tcggtttcca cttcaatatc
     6541 caattcggaa tcaatgtcat ccggcagaga cgtatcctca atatccgtat cggattcctg
     6601 gctaagaatc tcctctgcat cgacatcaac ggggaaggcg ttctttttct ttttggcatc
     6661 aagtcctttt tcgacagccg attctttttt aggccttccc ggcttctttt tggcctcaga
     6721 cggcgccgcc ttcttcaccg attttgtggt gggagcagac ttggtacgat tccccccggt
     6781 tttcgccgca ttggtcgttt tcgaaacatt gtcagatgta gtcaacttct tctctttccc
     6841 tttcaccgcc tttttcggcg tcgttttgga tcgtggttga gcttgagcca cggctttgga
     6901 tgtccccttc actcgagcct tcgtatttcc tttggcttga gtcttaacgg gtcccttggt
     6961 cctagccttg gagccgacct tcgtattctt ggacttcgct ttggtagacg gtgatcgtgg
     7021 aggcttcaca gacttctttg gtttggcttt tgtcttcggt tgagctttcg catgagtggt
     7081 tcgcttcccc gtcggcttct gtttgggctt ggcctttgac ttgggcttag ccatggcagc
     7141 catgtgtcac cctcctgatt atgtgatcga caaatgatcc atctgcgatg gatcgtctcc
     7201 tgagttgccc ccatctaaca tggagagccc ctctcaagca acagaagatt ttcaagaagg
     7261 aaattctttg ccttttttag aaacttccgg tcattcaaca tccctgttag aatcgggtct
     7321 ccatggaaat tattaccacg catcttgagg cggatttcga tggccttgcc tcaatggtgg
     7381 ccgctcaaaa gctctacccg gaggcacacc tcgtcctccc ggctggagtc caatcccggg
     7441 agcgggcctt tctcgcggaa catccccttt ccttcacggc actttcgagt ctaaattttc
     7501 cggatatcac ccgcctgatt ttagtcgatg cccaacacca ggatcgctta ggcttactcg
     7561 aaaaagtcct taaaaatcaa catatttcag ttcacattta cgatcatcat ccattggagg
     7621 ccaccaatcc tctgctcccg atggcagact attgcaaggt ggattcggtc ggggccacca
     7681 tcacactcct ctgcgaggaa cttcaaacca gaggactcac atggacaccc gaggaagcca
     7741 cattctttgc catagccctt tatgaggaaa ccggtcaatt tggctacccc aataccacgc
     7801 cccgtgattt ccagatgggt ggggtccttg tgcaaacagg agctgattta aaccaggtca
     7861 cgaagtacat cgcccggcac tggacgccgc cgcaactgga attattccat gcactgttgc
     7921 aatcggcgca aacactcaac ctcggacatc gaaggattct gctcaccacc ctcacctggc
     7981 cggattatgt gcaggacatg gccccggttg ttcaacaact agcgcaatta cacggagccg
     8041 atgccatcat cgccgctgtc gccatggatg gcaaggtaca aatcatcggg cgcagccgcc
     8101 atcccgacat ggacatgaat cagatcacca aggcctttgg cggaggaggg cacaccatgg
     8161 ctgccgccgc cagcattaag ggactcacca tcgtcgaggt cgaacagaaa atccacgccc
     8221 ttttgcagga acaagccaaa acctggcttc ccatcaaacg tctcatgaca acacctgtca
     8281 ggacggt
//
LOCUS       DATIAL010000045         6646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_653797, whole genome shotgun sequence.
ACCESSION   DATIAL010000045 DATIAL010000000
VERSION     DATIAL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653797"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VLA60_01485"
     CDS             complement(<1..502)
                     /locus_tag="VLA60_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HSF08052.1"
                     /translation="MKKMKAVEVRQAKGSLQLVEREVPNPGSGQVLVKVQACGICHSD
                     VFTKEGLWPGLEYPRIPGHEIAGIIEEMGSGVEGWKQGQRIGVGWHGGHCGHCEPCRR
                     GDFVLCQRGLVPGISYDGGYAEYMIAPVEALARIPEDLSDVEAAPLLCAGITTFNALR
                     KSGARAG"
     gene            849..1211
                     /locus_tag="VLA60_01490"
     CDS             849..1211
                     /locus_tag="VLA60_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSF08053.1"
                     /translation="MNALSLPGVKRRMIQQWPHFHLSPHRRHEQGHEVVLWLQAEAVV
                     LAKKGVVDGVQGAGLPALKVLANTNTILSQGIPVWVCSTCATARQINEPDLEVGAMMK
                     GMQDYVKAITESDRNLSY"
     gene            complement(1256..2659)
                     /gene="fumC"
                     /locus_tag="VLA60_01495"
     CDS             complement(1256..2659)
                     /gene="fumC"
                     /locus_tag="VLA60_01495"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSF08054.1"
                     /translation="MKKKTASIRIETDSFGSIPVAADKYWGAQTQRSLENFQIGQERL
                     PRPLIRALGIVKRCAALSNIMLNTIDETTGKAIADAAQDVIDGKLDDHFPLVVWQTGS
                     GTQSNMNANEVIANLAIERLGGTLGSKTPVHPNDHCNLSQSSNDVFPTAMHIATVEQL
                     HHVLIPSLAHLHQVLLDKSQAWEGIIKIGRTHLQDATPLTLGQEFSGYAAQLRLGIDR
                     IQDGLKRLYPLAQGGTAVGTGLNAKPQFGVKFAEEVARYTQLPFLSASNKFEALATHD
                     AMVEISGVLNVIAVSLNKIANDIRLLSSGPRSGLGELSLPENEPGSSIMPGKVNPTQC
                     EAMTMVCAQVIGNHTTITISGAQGHLELNVFKPVIAYNVLQSIRLISDAVMSLTDKCI
                     VGIEPNMGRISELMEQSLMLITALVPHIGYDKAAKIAQGALRTGRTLREEAIASGFVT
                     EEEFNTFVRPEQMIRPK"
     gene            complement(2681..3229)
                     /locus_tag="VLA60_01500"
     CDS             complement(2681..3229)
                     /locus_tag="VLA60_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HSF08055.1"
                     /translation="MENIFFDNWQALGRTGLISVLAYLCLLMLLRMFGKRTLSKMNAF
                     DLVVTVALGSTLATILLNKDVTLAQGVLALVLLIGMQFVVTWSSVRVRWVRKLATGEP
                     TMLLYQGAFLPAALRWTRVTEDEVRAAVRNAGAATLDEVKAVVLETDGSFSVVRSGGG
                     SGESSLTGVSRPSGIPPTHTNR"
     gene            3743..5251
                     /locus_tag="VLA60_01505"
     CDS             3743..5251
                     /locus_tag="VLA60_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF08056.1"
                     /translation="MRIKDPVLRNTPITLTATFQLALLIFCGLVLIGQQRALGKEQKT
                     VIPDQDISRAVESALLLDENIPSHLIDVRTDQGIVTLIGVMPHYRAKDRAAKIVETVK
                     GVASVINGLTVKISNKTDKEIRENVQKALQDDPATDSYGIDVAVEHGTVTLTGTVPSW
                     AARELSEWVATGVHGVREIQNNLFIDLKEKRSDDVILDEIQKRLEADVWVNEDAVLVS
                     VNNAEVKLDGIVGSIAEKRRVLSDARVAGVREVDDDLLFVKAWAKGQMRRQGQPGYKS
                     DEEIKETLILAYAYDPRVAVFNPTIDVRHGMVTLTGTVDNLKAKQSAEEVAKYTRGVV
                     WVKNLLKVRPKEPVDDEALARKVEMALHEDPFIDRFQVIMTVHNGIVVLRGSVGSQFQ
                     KDQAQDTVSRVKGVVDVKNKLQVDEIWTWKPDIAIQREIENELWWSPFVDSDDITVSV
                     KAGVATLSGTVDNYFEYEAALENAREGGARRVESLLTIRRYLNFDILPDIPL"
     gene            5289..6218
                     /locus_tag="VLA60_01510"
     CDS             5289..6218
                     /locus_tag="VLA60_01510"
                     /inference="COORDINATES: protein motif:HMM:NF022391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HSF08057.1"
                     /translation="MKGGVHQYKMVWLFTLLFGVTTGLVTSAVLAAKPRIESKITVES
                     KEQLTASEKASLSRAAGRILLHVDDARKALQGDQTEDALTHIEKGLILVRIIEESSPD
                     YEVKATITSGALTYEDIRKHRQLFVPVYEELDEISSLGPVIAAKNEQAKQDKSAIPVV
                     EDVRIRYTRAELNVALAKVGLEQAKKALKSENTEKADDALAAIQTGVTLNYAEIDLPL
                     EEARKNLLLAQGMIEHNQAKEARAALEEASEDLKQYEKSIGELRGEKVKHLRQEIRDV
                     MSTIENTQKEKKGSNSLKEQIEGWWNSVTEWLS"
     gene            complement(6277..>6646)
                     /locus_tag="VLA60_01515"
     CDS             complement(6277..>6646)
                     /locus_tag="VLA60_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08058.1"
                     /translation="GGVGINLAIQDAVAAANALASNLRNGNPIQETLFREVQQRRLLP
                     TRIIQALQVQAQNRLISKALERSGDPPEFPAILRWLLQFRVVRNIPARLIGYGICREH
                     VRMRRMPSRILKCFIASVPG"
BASE COUNT         1822 a         1637 c         1685 g         1502 t
ORIGIN      
        1 caccggcacg tgcgccgctc tttcgaaggg cattgaaggt cgtaataccg gcacaaagca
       61 gaggggccgc ttcaacgtcg gagagatcct ctggtatacg cgccaaggct tcgacgggag
      121 caatcatgta ttcggcatag cctccatcat agctaatccc cggaaccaac ccgcgttgac
      181 aaaggacaaa atcgcctcgg cggcaaggct cacaatggcc acagtgtcct ccatgccacc
      241 ccacgccaat ccgttggccc tgtttccatc cctccacacc cgaacccatc tcctcgatga
      301 taccggcaat ttcatgacca ggaatacgcg gatactccag accgggccat agaccttcct
      361 tcgtgaacac atcgctatga cagataccgc aggcctgaac cttaacgaga acctgtcctg
      421 agccaggatt aggcacctca cgctccacga gttgcaaaga gccctttgcc tgccggactt
      481 ctacggcttt catctttttc atgtgaatac tccttattta ttcactataa aatgactccc
      541 gccttggaga ggatcgaacc cacaagcctc aggcgggttc cgaataatgt cccgccatct
      601 atcatgacag atcaggaaac tctaagaaaa gtggatatag acggaaccgg ggagccaaca
      661 tttttcaccc gcgccatcta aaaccaaggg ttctacagag gtgaggctta aatttttggc
      721 taatatcatc aagttacagc acaggtaccg ggcgactgca tcttaatcct aatccaactt
      781 tcctgtgcaa tgagatgggg ggttgcggag aagcatgaaa gaaaagaggt tcatgaaagg
      841 agaaaactat gaacgccttg tcattgccgg gagtaaagag acggatgatc caacaatggc
      901 cacacttcca tttatcgcct cacaggcggc acgaacaagg gcatgaggtc gttctatggc
      961 ttcaggccga ggccgtcgtc ttagctaaaa aaggtgtggt agatggtgta cagggagccg
     1021 ggctaccggc attgaaggtg ttagccaata ccaataccat tctgtctcag ggtattcctg
     1081 tgtgggtttg ttcaacctgt gcaacggccc gacaaataaa cgaacccgat ctcgaggtgg
     1141 gtgcgatgat gaagggcatg caggattatg taaaagccat taccgaaagc gatcgcaatc
     1201 tttcgtatta aattcctttc cggaaggagc aagaatgccg ctggggacgt tggggttact
     1261 ttggacgaat catctgttcc ggacggacaa aggtattgaa ttcttcttca gtcacaaatc
     1321 cgctggcaat agcttcttct cgcagggtgc gcccagtccg taaagcccct tgagcgatct
     1381 tggcggcctt atcgtagccg atgtgaggga ccaatgccgt gatcagcatc agagactgtt
     1441 ccatcagctc ggaaatacgg cccatatttg gctcaatgcc cacaatgcat ttatcggtca
     1501 ggctcatgac ggcatcgctg atgaggcgga tggactggag cacgttgtac gcaatgacgg
     1561 gtttgaacac attgagttca agatgcccct gtgcgccgga aatcgtgata gtcgtgtgat
     1621 tgccgataac ctgcgcacag accatggtca tggcttcgca ttgagtggga ttcacctttc
     1681 ccggcatgat ggaagagccc ggctcatttt ccggcaggga gagttctcca agaccggagc
     1741 gcggaccgga acttaacagg cgaatatcat tggcgatttt attcagactg acggcgataa
     1801 cattgagcac gccggatatt tccaccatgg catcatgcgt ggccaacgct tcgaacttat
     1861 ttgaagccga aagaaagggc agctgcgtat accttgcgac ttcttcagca aacttcacac
     1921 caaactgggg ctttgcattc aatcccgtac ccacagccgt accgccttgc gcaaggggat
     1981 acagtcgttt aagaccatcc tgaatccgat caatccccaa tcgcagctga gctgcatatc
     2041 ctgaaaattc ctgacccaac gtgagaggag tggcgtcctg caaatgggtt cgcccgattt
     2101 tgataatgcc ttcccaggct tgcgacttat ccaacaagac ttggtggaga tgtgccaagg
     2161 atggaatcaa gacatggtgc agttgctcaa ccgtggcaat atgcatggcc gtcgggaaaa
     2221 catcattgga agactggctg agattgcagt gatcattcgg atggaccggc gtttttgatc
     2281 cgagcgtccc gcccagacgt tcgatggcca gattggcaat cacctcattg gcgttcatat
     2341 tggactgcgt accggaaccg gtttgccaga cgactagggg aaagtgatcg tccaacttgc
     2401 cgtcgataac atcctgtgcc gcatcagcaa tagccttgcc ggttgtttca tcaatggtgt
     2461 tcagcatgat attcgatagg gccgcgcaac gcttgacgat acccaaggcg cggatgagag
     2521 ggcgaggcaa gcgttcctgt ccgatttgaa aattttccaa ggaacgttgg gtctgagccc
     2581 cccaatattt atccgccgcc acaggaatag aaccaaaaga gtcggtttcg atacgaatgc
     2641 tagctgtctt ttttttcatg gctcccccta acggcatagt ttatctgttg gtgtgggtgg
     2701 gtggaatgcc ggacggtcga ctgacccctg tgaggctcga ctctccggaa cccccgccac
     2761 ttcggactac actgaaggag ccgtcggtct ccaacaccac cgccttgacc tcatccaacg
     2821 tcgccgctcc ggcgttccga accgcggctc gcacctcatc ttcagtcacc cgcgtccaac
     2881 gcaaggcggc aggtaaaaat gcaccctgat ataacagcat cgtcggttcc cccgtggcca
     2941 actttcgcac ccatcgaacg cggacactgg accacgtcac aacaaactgc attcctatca
     3001 gcagaaccaa ggccaggacc ccctgcgcca aggtcacgtc tttattgagc agaattgttg
     3061 ccagtgtcga gccgagagcg acagtcacga ccaagtcgaa ggcgttcatt ttggataagg
     3121 ttcgtttgcc gaacattctg agcagcatta gaaggcatag ataagccaac acactaatga
     3181 gccctgtccg gcctaaggcc tgccaattgt caaaaaagat attttccaat tttatgtccg
     3241 gtgcaccgca ctacctctaa gcggcatcca catcgattag gcatcatatt cttcaagcct
     3301 atactatcta ctgggatcct ccacggattc ttggttattt gaaggggtta gtcaaggaaa
     3361 agatcggcaa gcgaagaatt cacgaccatg tcaacccacc tctaggaaac attcaagaaa
     3421 gtaactgatg gaattaccac gctaatccac gtcaccgtcg tgggaaagac caggggtttt
     3481 tatagttccc atcatgcttt gcttccccta acatatgaaa aattgaaaaa tggcggagat
     3541 gccatcttac taatcgtccg tgaggaaggc gctccctatg tttgcgcaaa tataccttcg
     3601 tggagaattt gttttaacca aattcaaacg ctccgaatgc gccctatgac actcgtcctg
     3661 aaggtgatat taaatacgaa attatcaacg aagacccttc gacccaattt tgatatagga
     3721 gaaaacattt aaggaaactt ccatgcgaat aaaagatccc gttctgagaa atacgcccat
     3781 caccctcaca gctacatttc agttagctct gctgatattt tgtgggctcg tcctgatagg
     3841 tcagcaacgt gcattaggga aggaacagaa gactgtcatt ccggaccaag atatctcccg
     3901 agccgtagaa agcgcccttc ttcttgatga gaatatccca tcacacttga ttgatgttcg
     3961 cacggatcag gggattgtca ccttaatcgg agtcatgccg cattatcgcg cgaaggaccg
     4021 agcagccaaa atcgtcgaaa ccgttaaagg agtagccagc gtgataaacg gtctcacagt
     4081 gaaaatttcc aacaaaacgg ataaagaaat tcgcgaaaac gttcagaagg ccttgcaaga
     4141 cgatccagcc acagattcct atggaattga cgtagctgta gaacatggaa ccgtaacgct
     4201 cacaggaaca gttccgtctt gggcagctcg agaactaagc gaatgggtag ccactggtgt
     4261 tcatggtgtg cgagagatac agaataatct tttcattgat ctcaaggaaa aacgatctga
     4321 tgacgtcata ctggatgaga tccagaaacg gttggaagct gatgtctggg tcaatgaaga
     4381 cgctgtgttg gtctccgtga ataatgcgga agtaaaactt gacgggatcg tagggagtat
     4441 cgccgaaaaa cggcgagtgc tcagcgacgc acgagttgca ggagtcagag aagtcgatga
     4501 tgatctcttg tttgtcaaag cctgggccaa aggccaaatg cgacgccagg gacagcccgg
     4561 gtataaaagc gatgaagaga tcaaagagac cctgatcttg gcatacgcct acgacccacg
     4621 agtggcggtg tttaatccca ccattgatgt tcgtcacggc atggtcacgt taaccggcac
     4681 ggtcgacaat ctcaaagcga aacaatccgc ggaggaggtg gcaaaataca cccgtggggt
     4741 agtctgggtg aaaaacttat taaaagtcag gccaaaggag ccagttgatg atgaagcact
     4801 tgcacgaaaa gtcgagatgg cactccacga ggatcctttt atcgatcgtt ttcaagtgat
     4861 tatgactgtt cacaacggga tcgtcgtgtt acgaggctca gtcggctctc agtttcaaaa
     4921 agatcaggca caggataccg tttctcgagt aaaaggtgtg gttgatgtga aaaataagtt
     4981 acaggtcgat gagatatgga catggaaacc ggatattgcc attcaacggg agatcgaaaa
     5041 cgagctatgg tggagcccat ttgtggatag tgatgatatt acagtttccg tcaaggccgg
     5101 cgtggccaca ctttccggca ccgtcgataa ttattttgaa tacgaagcag ccctggagaa
     5161 tgccagagaa ggcggcgcca gacgtgttga gagtttgctc accataagaa ggtacttgaa
     5221 ttttgatatt ctccctgaca tccctctgtg atgatccaaa gcataaatag tacagaaagg
     5281 aggaatggat gaaaggtgga gttcatcaat acaagatggt gtggttattt acgcttctgt
     5341 ttggtgtcac aaccggatta gtcacatctg cggtgctggc agctaaacca agaattgaat
     5401 ccaagattac ggtcgagtcc aaagaacagt taaccgcatc tgaaaaagcc agtctgtccc
     5461 gagctgccgg gcgcattctt cttcatgtag atgatgcacg aaaagctcta cagggagacc
     5521 aaaccgaaga tgcgctcact catatcgaga agggcctaat cctggttcgt atcattgagg
     5581 aaagctcccc ggattacgag gtgaaggcaa caatcacatc cggcgctctc acgtatgaag
     5641 acataagaaa acaccgccag ttgtttgtgc cggtgtatga agagttggat gaaatatctt
     5701 cgcttggtcc ggtgattgcc gccaaaaatg agcaggcgaa acaagacaaa tcggccattc
     5761 ccgttgttga agatgttcga atacgctata cccgcgctga gctgaatgtg gcactagcca
     5821 aagtcggact agaacaggca aaaaaagctc tgaagagcga gaacaccgag aaagctgatg
     5881 atgcgcttgc ggccattcag acgggagtga ccctcaacta tgctgagatc gatcttcctc
     5941 ttgaagaggc acgcaaaaac ctattgctgg ctcaagggat gattgaacat aatcaggcca
     6001 aagaggcccg tgcagccctc gaagaagcgt cagaggattt aaaacaatac gaaaagagca
     6061 tcggggagtt gcgcggagaa aaagtgaagc atttacgcca agaaattcga gacgtcatga
     6121 gtacaataga gaatacgcag aaagagaaga aaggttcaaa cagcttgaaa gaacaaatcg
     6181 aaggctggtg gaattccgta acggaatggt tgagttaatt tttcgtttac aataatgctc
     6241 tttcaaaaat aacacaaaaa atttgacaat ggtgtcctat cctggaacag aggcaatgaa
     6301 acacttcaat atgcgggagg gcattcgcct catgcgcaca tgttcccggc aaatgccata
     6361 gccgatcaag cgggcgggga tatttcgtac gacgcggaac tgtaacagcc agcgcagaat
     6421 cgccggaaat tccggtgggt ctcccgatct ctccagggcc ttggagatca gacgattctg
     6481 agcttgcacc tgcagagcct ggatgattct ggtcggtaac agacggcgtt gctgtacctc
     6541 gcgaaacagt gtttcttgaa tcgggtttcc gttccgtaga ttcgaagcga gagcattcgc
     6601 agcggcgacg gcgtcttgaa tagccaaatt gatcccgaca cccccg
//
LOCUS       DATIAL010000046        10338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_116457, whole genome shotgun sequence.
ACCESSION   DATIAL010000046 DATIAL010000000
VERSION     DATIAL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116457"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..444)
                     /locus_tag="VLA60_01520"
     CDS             complement(181..444)
                     /locus_tag="VLA60_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08059.1"
                     /translation="MGHSLEQLQKIADDLKRQRDELQVKLHLAKADARDEWAKLETRW
                     EDVKTKMGAVRKEASHTTESVSSGLGLVLDELKKGYDNIRKTL"
     gene            complement(948..1352)
                     /locus_tag="VLA60_01525"
     CDS             complement(948..1352)
                     /locus_tag="VLA60_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08060.1"
                     /translation="MRRTIPIHALAIVLALILIGGEIVTAEMPPPEPDEAYLVDEDIN
                     IVTGLYIREYSLRKDGIVDYKTARQILISEYNEHWNSVVETKEWPLFYWHDVNRDGHW
                     TMYVDRKVKGCTCDIVPYEVKTEDAIAQQDPF"
     gene            complement(1610..2581)
                     /locus_tag="VLA60_01530"
     CDS             complement(1610..2581)
                     /locus_tag="VLA60_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSF08061.1"
                     /translation="MNYVHLGRSGLKVSRLCLGTMNFGPETTEADSGTIMDTALELGI
                     NFFDTANVYGWEAGEGITEQIIGRWFAQGGQRREKVVLATKVFGRMGDWPNQSRLSAL
                     HIKRACEASLRRLQTDYIDLYQMHHVDRHSPWEEIWQAMEQLMREGKILYVGSSNFAG
                     WHLAQAQEMARHRNFLGLVAEQSLYNLNDRMIELEVIPACEAYGIGLIPWSPVGRGLL
                     AGALEPATSGRRADEDLRKNVEKFRPRLEAYETLCRELGEAPAHIAMAWLLHQPAVTA
                     PIIGPRTLDQLQGAMRTFDITLSKEVLKKLDTIFPGPGGPAPEAYAW"
     gene            3079..4032
                     /locus_tag="VLA60_01535"
     CDS             3079..4032
                     /locus_tag="VLA60_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSF08062.1"
                     /translation="MPILLNVGVDVGKDTLVVACAENHFPVHTVPNQRGPLLAWLKSL
                     PRGSRIGLESTGHYHELLADLAQTHGDCVFLLNPRETRHYAKAMGNRAKPDRVDAVLI
                     ARLIAQEHTRLRAYMPPTATQRKLDRMIRRRATIVRLKGTLKLTMRNLGGFATELKAV
                     GARLDALLAKMDRTMAAMAAESPHHQAAQHRVETIVGVGPLVGIALTNTLERVPFRTA
                     DAFVAFTGFDPRANDSGHRVGRRRLSNRGPAELRRLLYNAAMSAIKTTVWKPIYEPYR
                     TRGWSTTATLVIIARKIARTAWSIHHYHTTFNPERITQCLT"
     gene            4815..5231
                     /locus_tag="VLA60_01540"
     CDS             4815..5231
                     /locus_tag="VLA60_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08063.1"
                     /translation="MPNSVRLVKYYKMMVPHKSGQGMKLLEHLQQSQVNLLAFLAFPK
                     KNGKAQVDCVPVDPAKFTAAAKKAKWNIKGPKACFVIDDKDQVGAFIPYAAKLAEAKI
                     NIRAAAAVTGGAGRCGAILWVNQKDVKKAAKALGAE"
     gene            5697..6440
                     /locus_tag="VLA60_01545"
     CDS             5697..6440
                     /locus_tag="VLA60_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08064.1"
                     /translation="MKGFERYQRILINEVDKFLNKESLIENSKYQNAFKNHIFGLSGP
                     KQFSKKQIPSEVFFTKLFYGFIEISDSYSCLKDTETYIGSFPYRNAKISQTRYLAYHF
                     ENYLNEIYLLKERLRSYLKSIGRQYKNDRRYRIISKNTSSLTDWVDNCLKGIINSRGS
                     HVHQTRFSAEDLDRLKTQEFLTLHGGKELKIIKDLFKRDYGNTRKKYKQIIKENNQQV
                     KIFLDTFFVILMKIVANNEGRIRYPQIGN"
     gene            complement(6789..7124)
                     /locus_tag="VLA60_01550"
     CDS             complement(6789..7124)
                     /locus_tag="VLA60_01550"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HSF08065.1"
                     /translation="MQPTQTSETNSPSQIEPAIQHVVPWRVVSVSTKSDLRLQVTFVD
                     GTTGEVDLKKFLNDANVRGTVFESLRDPVAFSKVKVVMGAVQWANGADLAPDAMYDAI
                     KSQGRWVVE"
     gene            complement(7079..7339)
                     /locus_tag="VLA60_01555"
     CDS             complement(7079..7339)
                     /locus_tag="VLA60_01555"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HSF08066.1"
                     /translation="MPTISIFYGILIQMFWREHNPPHFHALYGEHEALIDVRELRVLR
                     GSLPRRAMALVLEWAAEHRDELMEDWNLCSQLKHPKPIPPLK"
     gene            complement(7630..8799)
                     /locus_tag="VLA60_01560"
     CDS             complement(7630..8799)
                     /locus_tag="VLA60_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08067.1"
                     /translation="MKNTSTIRAIMTGFAERTGLIPPARPPRRYLWTDAFAVCNYLEL
                     FRQTGKEQYQQDAQRLVSEVHRVLGRHREDDSRRGWISGLDEQEGEQHPTRGGLRIGK
                     ELGERGPNEPYDEHLEWERDGQYFHYLTKWMHALDCLARVTGDAVYNRWALDLAKTAH
                     SRFAYNPWGGGPKRMYWKMSIDLSYPLVPSMGQHDPLDGLITYQQLQATAMKSAEPHA
                     ESPLGSEIAEMTVMCEGMTWTTDDPLGIGGLLSDAFRLAQLIISSNLRESERLASLLR
                     DAEVGVEAFVRSHPLNASADHRLAFRELGLSIGLHAIVKMQRIIEQHPEKFSNPQELQ
                     ASLSGLARFLPLIESIERFWLEPNNQQSHTWTGHREINSVMLATSLAPDGYLVLQ"
     gene            complement(9209..9403)
                     /locus_tag="VLA60_01565"
     CDS             complement(9209..9403)
                     /locus_tag="VLA60_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08068.1"
                     /translation="MQRAFKDIVCRPERACLHHIIPSTRSSSTSPGFGYEWGRLFDPK
                     QVVHQDTVALDLGMQVAGHW"
     gene            9471..9977
                     /locus_tag="VLA60_01570"
     CDS             9471..9977
                     /locus_tag="VLA60_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF08069.1"
                     /translation="MVRDKTLLVGVDGSDSSIRSVSYVAEMIGAREDFHIVLLHILPP
                     IPPDLLEFGGAEDPVIEQKLDETLKKEQAQWIEKAKQDAEPTLDNAKTILYRIGVLPA
                     MVNVVFSQSIHRPDIVGELIGTAHKQNCGTIVVGRETYSSFKEIFHHHVGEELAKKGQ
                     GFAVWVIA"
     gene            complement(10159..>10338)
                     /locus_tag="VLA60_01575"
     CDS             complement(10159..>10338)
                     /locus_tag="VLA60_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08070.1"
                     /translation="PLLCKEGAGEVETPYRNCWPNFPIASKMPKDPRLYLTLVLPLQR
                     GGMTGSLFGRKYVTE"
BASE COUNT         2550 a         2647 c         2604 g         2537 t
ORIGIN      
        1 ggtggtttcg ggttttaacg cggtatggtc aataatttta gtgaattcgt cgcgggtcat
       61 cctgcaggct ccgatgagag tttcgagagg ttgcggtgtc ccctccgaga attcgtatga
      121 cctgaccgtg tcgtattgat acccggatca gacaacaaat atgagctgaa agggcacgga
      181 ttacaatgtc ttgcgaatgt tgtcgtagcc ttttttcagt tcgtccagca ccagtcccag
      241 cccggaggat acggactcgg tggtgtgact ggcttccttt cggaccgctc ccatcttggt
      301 cttgacgtct tcccaccgtg tttcaagttt cgcccattca tcacgcgcat cagctttggc
      361 caggtgtagt tttacctgaa gctcgtcgcg ttggcgtttc aaatcatcgg caattttttg
      421 aagctgttct aacgaatgcc ccatgcatgt ctcctttcct tgaaaccctg cataaccggt
      481 tgatgaatct tgtactagat tagcaagaaa ccggaccgag agtgtactcc gccacaaggg
      541 cgaatccatt caccggccaa tcagcgattc ctttgtggtg caggaaggca ggagccgtgt
      601 cgaatcccgg gagtaggcga gggagcgtgg cagctggtct tgtgaagtga gctgttgaaa
      661 aacttaaaat tcttctgaga gcttatcgcg ggaaccaaaa attttcgatc ttccaagagc
      721 aaaggaggat gttgaaaaag gcccgtcctg tcctgggggg tacgtgagca cggctaaggg
      781 gcgacctgcc tgcgcgcttg gtcgtcgaag cgtttcagcg cgtagacgtg aaaccgcttt
      841 cagccttcgt agctacttcg gcgaaggagg cctggcgaag gcagggaacg ccgttggcgg
      901 ctccttctcg gccagactca gggcatgctt tttcaacact ctgctggtta aaaaggatct
      961 tgttgggcaa tcgcgtcctc cgtcttcact tcgtagggga cgatatcgca ggtgcagcct
     1021 ttgacctttc ggtccacata catggtccaa tgtccgtccc ggtttacatc atgccagtag
     1081 aataacggcc attctttggt ttcgacaacc gaattccaat gttcattata ttccgagatg
     1141 aggatttggc gggcggtttt gtaatcgacg atgccgtctt tgcggaggga atattctcga
     1201 atgtagaggc cggtgacaat attgatatct tcgtcgacca ggtacgcctc atccggttcg
     1261 ggtggcggca tttccgctgt gacgatttct cctccaatga gaataagggc taaaacgatg
     1321 gcgagtgcgt gaatggggat ggtccttcgc atgacatcct ccctggcttg aggccctgag
     1381 cccgactcca tctggttgtg agtggacttc ctctctgttc cttagagcaa ctcgtatgcc
     1441 aaagatgacc tggacggttg gtggtgattc agacgcggac aacctattga gttgtaagga
     1501 attcgaatgg tggtgaggcc ggtgtgggga aaacgcactc gtcaatattt tgtcgatgat
     1561 ttatctcttt atgcacaagg tgcaaatatt tgacacccgg cgagggctct caccaggcgt
     1621 aggcttcggg tgcggggcct cctggtccgg ggaagatggt atctaatttt ttcaacacct
     1681 ctttggacag tgtgatgtcg aaggtgcgca tggcgccctg taactggtcc agcgttcggg
     1741 gaccaatgat gggtgccgtg acggctggtt gatgaagcag ccatgccatg gcgatatgtg
     1801 caggcgcctc gcctagttcc cggcacagcg tttcgtaggc ttccaaccgt ggccggaatt
     1861 tttctacgtt cttgcgcaga tcctcatcgg cccgtcgtcc gctggtcgcg ggttcaagcg
     1921 ccccggccag aaggccgcgc ccgaccggac tccagggaat taagccaatg ccgtaggctt
     1981 cacaggccgg aatgacctcc agctcaatca tgcggtcatt taagttgtac aaactctgct
     2041 ccgccaccag tcccaggaaa ttccggtgcc tggccatttc ctgcgcttgc gccagatgcc
     2101 atcctgcaaa gttactgctc cccacgtata agattttccc ctcgcgcatg agctgttcca
     2161 tggcctgcca gatttcctcc catggcgaat gccggtcaac atggtgcatc tgatacaggt
     2221 cgatataatc ggtttggagc cggcgcaaac tggcttcaca agcacgcttg atatggagtg
     2281 cggacaaacg ggattggttg ggccagtctc ccattcgccc gaacactttg gtggccagga
     2341 cgactttttc ccggcgttgg cctccttgcg caaaccaacg accaatgatc tgttcggtaa
     2401 tgccttcccc ggcttcccag ccatacacgt ttgcggtatc gaaaaaatta atgccgagtt
     2461 ccagtgcggt atccatgatc gttccactgt cggcttctgt ggtttccggt ccgaaattca
     2521 tcgtgcccag acacaaccgg ctgactttca acccggatcg accaagatgg acatagttca
     2581 ttcagcccct cctcataata gatgacgcac acagcggaaa agcactacag cactctagca
     2641 gggtgttgaa aaagtccgcc agcggcgttc cctgccttcg ccgggcctcc ttcgccgaag
     2701 tagctacgaa ggctgaaagc ggcttcacgt ctacgcgctg aagcgcttcg acgcgcaagc
     2761 gcgcaagcag gtcgcccctt ggccgtgctc acgtacttct ctgtacgctc cgcccgtcca
     2821 agcggctgcg gcctttcctt cgagatgcct tgtatggtgt ctacggctca tttttgagaa
     2881 gttaggagga actggggtgg agggacatcg agaaacatct gccgcacggc ggacagactt
     2941 cctgagaact ctcccaccca caccgtcaac gtcgatgaag aatctagcgg cacagggatg
     3001 ctgaccgccg atttgtccgc aagcttacgt tgtgtgtgcc accccagccc tattcattta
     3061 cttctggagg atacgctcat gccaatcttg ctgaacgtcg gcgtggatgt gggcaaagac
     3121 accctggtgg tggcttgtgc cgaaaaccat tttcctgtcc acactgttcc caatcagcgt
     3181 ggccccttgc tggcttggct gaaatctctc ccccgtggca gccgtattgg tttagaatcc
     3241 accggccact atcacgaatt actggctgac ttggcgcaga cgcacgggga ttgcgtcttt
     3301 ctgctcaatc cccgggagac tcggcactat gccaaggcca tggggaatcg cgccaaaccc
     3361 gaccgggtgg atgccgtctt gatcgcccgg ctcatcgccc aagagcacac gcgcttgcgg
     3421 gcctacatgc cccccaccgc cactcaacgc aaactggatc ggatgattcg tcggcgcgct
     3481 acgatcgtcc gcctcaaagg caccctcaag ctgacgatgc gcaatctggg cggctttgcc
     3541 accgaactca aggcggtggg ggcccgattg gatgccttgc tagccaaaat ggatcgcacc
     3601 atggctgcga tggccgccga gtcgccgcac catcaggcgg cgcaacatcg ggtggaaacc
     3661 attgtgggag tcggcccgtt ggtgggtatc gccctgacca ataccttgga acgcgtaccg
     3721 ttccggacgg cggatgcctt cgtcgccttc acggggtttg accctcgagc gaatgactct
     3781 gggcacagag tcggtcgccg acgcttatcc aaccgggggc ccgcggagtt acggcggttg
     3841 ctctataacg ccgcgatgtc cgcgatcaaa accacagtct ggaaaccgat ctacgagccg
     3901 tatcgcactc ggggctggag tacgacagcc acgttggtca tcatcgctcg taagatcgct
     3961 cgaaccgctt ggtccattca ccattaccac accacgttta atccagaacg gatcacacaa
     4021 tgcttgacat aaaccataga atctcaggac aggctttttt gaacggcccc gaggcctctg
     4081 atttgcaacg tgcctgtggg tcaatatgcc cttggagatt agtattattc aacattccca
     4141 ctatcaggga aagggtactc atccatgcga caacagcccc tcattcaggt tcgtctgatc
     4201 atgcagggtt gccccttaaa catcgctgtg cacacacatt cttccccaac acgactcact
     4261 cctttttccg tttgaaaaat tcagcaaacg tgagacaacc ttacgacggc acattcttaa
     4321 gggattatac agaatgtatg gctccacaat ctttcagcag gatacctgaa atgttcatct
     4381 cccaatgtcc gacgcctgct gcccggccct ctggatggac gctctcacga gccggcaagc
     4441 cttgtttgag cgcagcgagt tggcttggcc ttccatgctt gacgttcatc tcattgaata
     4501 aggccggact gggcgtcact ggttttggct ccttttgccg gaacaaaagg ggctcggctg
     4561 ccgggccgaa acccggcaat acagaaaagc acggtgatac gcgagttcga gaaaaagaag
     4621 ggactcattc accgaccaac gcgtctcaac tcgaagatct caagatacat ttccgattaa
     4681 cataatattg ctgtaaagtc agaaccataa cttgtttatg atccttcttt agccatccca
     4741 gtcgaaatat tcaaagagta gaccagcaaa ctttctaagg attcacaaat ttttctaatc
     4801 aaaaggaggt ccccatgccc aattccgtcc gactggtcaa gtattacaaa atgatggttc
     4861 cccataaatc gggacaagga atgaagctcc tggagcacct acaacagtcc caagtcaacc
     4921 tgttagcctt tctggctttt ccgaaaaaaa acgggaaagc ccaagttgac tgtgtgcccg
     4981 tcgaccccgc caagtttacg gccgccgcca aaaaggccaa atggaatatt aaaggtccca
     5041 aagcctgttt tgtcatcgat gacaaggatc aagtgggcgc ctttattccc tatgctgcga
     5101 aactcgcaga agcgaaaatc aacatacggg cggccgcggc cgtgacaggt ggcgcaggac
     5161 gctgcggggc cattttgtgg gtcaaccaaa aggatgtgaa aaaagctgcc aaagccctcg
     5221 gcgctgagtg agggctttga gccaagaaca aactgcaata ttgctccagg atggcgcttg
     5281 ggatcaggag acccaaagaa tatagttatg ctcaaataat ctcaaaaaat aatgaatgtc
     5341 tttatgttta ggaggaaagc aacaagcagc cgattattgg accatctatc tcggcacgca
     5401 tcaagatgtg cacacgcgag cgcattggga agagagtcgc tgcgcggcgg gaataaactg
     5461 ctgggggtta atggcccagc gattttgcgt gtctccctct cggattttct gattcaacca
     5521 gcacttgagg ttggtgggtt atgtcgttag caagcccagg ttcggagatt gtgtcatgtt
     5581 aagcaatgcc tccggttctc gagccctgta atttgacaca atgtttgtta tattcaatgg
     5641 tgtgaattcc ccttggctct tcccttttag gcgcttcaat ccaaagtcgg tgatgtatga
     5701 agggatttga aagataccaa cgcatattaa ttaatgaggt cgataaattt cttaataagg
     5761 aaagtctaat agaaaactca aaatatcaga atgctttcaa aaatcatatt ttcggattga
     5821 gtggtccgaa acagttttcc aaaaaacaga tcccttctga agttttcttt acaaaattgt
     5881 tttatgggtt cattgaaata tcggattctt attcctgcct aaaagataca gagacttata
     5941 taggaagctt tccatatagg aatgctaaaa taagccaaac tagatatttg gcttatcatt
     6001 ttgaaaatta cttaaatgaa atatatcttt taaaagaacg tctgcgttcc tatttgaaaa
     6061 gcattgggag gcaatataaa aacgatagga gatatcgaat aatatcaaaa aatacaagct
     6121 ctcttaccga ttgggtggat aattgcctaa agggtatcat caattcaaga ggctctcatg
     6181 tacaccagac aaggtttagt gctgaggatc tggatagatt aaaaactcaa gagttcttaa
     6241 cgttacatgg tgggaaagaa ttaaagatta taaaggatct ttttaaaaga gattacggaa
     6301 atacacgaaa gaaatataag caaataatta aagaaaacaa ccaacaagtg aaaatttttc
     6361 tggatacatt ctttgttatt cttatgaaaa ttgttgcaaa taatgagggg cgaatcagat
     6421 atccccaaat tgggaattaa gttaactctt ttggaaacgc aaatagcctg agaccatttt
     6481 aatcaggcgt aaagaaaacc tctttcctgg ggtaaggatc gcaaagggcg aacctgtggc
     6541 ccccgaataa ggcattcgca ggcattgacg gataaggtta tcgttattga aataccagat
     6601 acccatcggg tgccaagctg gtcgccagca tcatgctgtt aatatcacga tgcccggtcc
     6661 caggtgtggc tctgttgatt gttcggctca agccagaaag cctcgatgag ttcggtgaat
     6721 ggaagaaatc gtgcgaggcc tgacagcctg gcatgcaact cttgttggca aggaaaagga
     6781 aagagggttt actcaaccac ccaacgtcct tgggatttga ttgcatcata catggcatcg
     6841 ggagccaagt cggctccatt agcccattgg acggctccca taactacttt caccttcgaa
     6901 aaggccacag gatcacgcaa cgattcgaat actgttcccc tgacatttgc gtcattcaga
     6961 aatttcttca gatctacttc ccctgttgtt ccgtctacaa aggtcacctg taaacgcagg
     7021 tcagacttcg tactgacgga aactacgcgc cacggtacga catgttggat agctggttct
     7081 atttgagagg gggaattggt ttcggatgtt tgagttggct gcatagattc cagtcctcca
     7141 taagttcatc gcgatgttcc gctgcccatt ccagcacaag tgccatagca cgcctgggta
     7201 acgatccccg caacacgcgg agttcacgta catcaatgag cgcttcatgc tcaccgtaga
     7261 gtgcatgaaa atgcggtggg ttatgctccc tccaaaacat ttgaatcagg ataccataaa
     7321 agatgctgat cgtcggcatg aatgaaccgt gttttattat gaatcatctg tgaatgacag
     7381 atagaatcac gtcgtgtgtt atcaaccttc agcactttat aggggagcag tttcctactc
     7441 ccaaatttgg tcccatctca aaattttggt catgtaagac ttaataaaat gctatggaaa
     7501 tcatgtagaa tatacaagat actgaattta tcaggaaagt taaactctta atccatctcc
     7561 ttgccagggc agtgtccagc cggattgtgc gtagttcact gaaaaatatg ctgggttatt
     7621 tagtgattgt tattgaagaa ccagataccc atccggcgcc aggctggtcg ccagcatcac
     7681 gctgttaatt tcacgatgcc cggtccaggt gtgactttgt tgattattcg gctcaagcca
     7741 gaagcgctcg atggattcga tcaatggaag aaaacgtgcg aggcccgaca ggctggcctg
     7801 taactcctgt gggttggaga atttctccgg atgctgttcg atgattctct gcatcttcac
     7861 aatggcatgg agaccgatgg atagccctaa ttcgcgaaag gccagacggt gatcagctga
     7921 agcattcaga ggatggctcc ggacaaaagc ctccacgccg acctcggcat cccgcaagag
     7981 cgaagccaat ctctccgatt cccgcaaatt agagctaatt attaattgcg caagcctgaa
     8041 ggcatcggac agaagtccac caattcccaa tggatcatcc gtcgtccagg tcatgccttc
     8101 acacatcacg gtcatctccg caatctctga ccccagaggg ctctctgcat ggggctcggc
     8161 cgatttcata gccgtcgcct gaagttgctg ataggtgatc aacccgtcga gtggatcgtg
     8221 ctggcccatg gacggaacaa ggggatagga gaggtcgata ctcatcttcc aatacatgcg
     8281 cttggggcca ccaccccaag ggttataggc gaagcgggaa tgggcggtct tggctaggtc
     8341 caaagcccac cggttataca cggcatctcc cgtcacccgt gccaaacaat cgagggcgtg
     8401 catccacttg gtcagataat ggaaatactg cccatcccgt tcccattcca gatgttcatc
     8461 atacggctca ttcggcccac gttcccccag ctctttgccg attctcagtc cacctcgggt
     8521 cggatgctgc tctccttcct gttcgtctaa tccgctgatc cagccacggc gcgaatcgtc
     8581 ctcccggtgt cgtcccagaa ctctgtggac ttcagagacg aggcgttggg catcttgttg
     8641 gtattgttcc ttgcccgtct ggcggaaaag ttcaagatag ttacaaacgg caaacgcgtc
     8701 ggtccagaga tagcgacggg gtggtcgagc tgggggaatc aggcccgtcc gctcagcgaa
     8761 cccggtcatg attgccctaa ttgtagatgt atttttcata gaccaacacc aaaaagcctg
     8821 ctctctggtt cttcttccat cgacgctcca ggcataataa gcaccccaca ggcacctgag
     8881 gctgacgcct gaacagaaga aatcgtcaac gcaaggtcgg ttgccgccaa ccatcagaca
     8941 aaatctgcgg ttccataagc ggacatttcc tccatcagtt caaatatctt cgttccaaaa
     9001 ggattcggaa tggagtattc ccaacacaaa ccggtttgtc tcatgatcaa agattgtgaa
     9061 gcgcttactc ctttcctccc cagcctgtgc acatgaactg tagcactccg ggacatacga
     9121 ggatatgcgc gtgtgaatgt tctcagattc tacaatattg tccagccctt caccccacag
     9181 gcgaacccgt gtagaattag agccgatttt accaatgccc agcaacctgc attcccaggt
     9241 ctaaggcgac agtgtcctgg tggacaactt gtttcgggtc aaacaatcgt ccccattcgt
     9301 agccaaagcc gggagacgtt gaggaagacc tcgttgaggg aataatgtgg tgaaggcaag
     9361 ctcgctcagg ccggcagacg atatctttga atgccctttg catgaacaaa atccgccaac
     9421 tatcctggtt agggggtagc gaaactcacg tcacatgaag ggagtgatct atggtaaggg
     9481 ataaaacatt actcgtcggc gtcgacgggt cggattcttc cattcgaagc gtctcctacg
     9541 tggctgaaat gattggggcc cgtgaagatt ttcatatcgt gctccttcac atcctccctc
     9601 cgattcctcc cgacctgttg gaatttgggg gcgcggaaga tccggtcata gaacaaaagc
     9661 tggatgagac attaaaaaaa gagcaggctc aatggatcga gaaggcaaaa caggatgcgg
     9721 aaccaacctt agataacgcc aagacgattc tgtatcggat tggggtatta cctgccatgg
     9781 tcaacgtcgt gttttctcaa tccatccacc ggccggatat tgttggtgaa ttgatcggga
     9841 cagctcacaa acagaattgc ggcaccatcg tcgttggacg ggaaacgtac tccagcttca
     9901 aagaaatatt tcatcaccat gtgggcgaag agctggccaa aaaaggccag ggctttgccg
     9961 tttgggtcat tgcctgatgg tgaatcaaga agcccgcacg gcctgggaaa atttcttcca
    10021 ttatcgaggg ttccggtatt ggtgaatgac caaaactatc cgacaggcga cacgggaatt
    10081 ccggccatat ttcagccatg attgtacata accatcgtga ataaaagaag atgctcttct
    10141 gggttttgtg tgagtcagct attctgtcac gtatttgcga ccaaaaaggc taccggtcat
    10201 tcctcccctt tgtaagggga ggactaaggt gaggtagagt ctcggatctt taggcatctt
    10261 gctggcaata ggaaaatttg gccaacagtt gcgatatggt gtttctacct cccctgcccc
    10321 ctccttacaa aggagggg
//
LOCUS       DATIAL010000047         8539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_224180, whole genome shotgun sequence.
ACCESSION   DATIAL010000047 DATIAL010000000
VERSION     DATIAL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8539
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224180"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VLA60_01580"
     CDS             <1..1780
                     /locus_tag="VLA60_01580"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HSF08071.1"
                     /translation="GGVARPPAQAMFCIDVRSEGFRRHLEEVGGNETFGFAGFFGLPI
                     CYQGFGSEQETDQCPVLLKPKHVVREIPRASQGKAAERFLERQALAKTGHALLHDLKE
                     NVITPYVMVEAIGWFFGFRLFGQTIAPAWYRTFTAWLKEWFAIPIATTLTVDKLSREE
                     AEEIVASEHRATIYRLLIERYGRLGLAVPHDQVDRLRRQALYQLEMDSTEEDSLGRLL
                     GWNAVSHEQFLGELRKDSGIHHRAVSRRMHRITQKGFTTNEQAYYVETALRIIGFTTG
                     FARLVLICAHGSESENNPYESALDCGACGGNEGISNARAFAAMANKPLVRQLLAQKDI
                     HIPPDTHFLPGQHNTTTDEVELYDLEDVPATHRKDLQRLQQDLEEAGHRNSQERLSRL
                     PDESGTPSLPRSLSLAKRRSWDWSQVRPEWGLSRHSTFIIGRRLLTRGVNLEGRTFLH
                     SYDYLQDPTGRYLEIIMTAPLIVGNWINMEHYFSTVDPEVYGAGSKVYHNVVGRIGVM
                     YGSQSDLRIGLPWQTVYDGPKPYHEPMRLFAIIEAPLERISGIIARQDLLQKLIGNQW
                     INLVALDPVTMEFFLYHSSEEWRIIL"
     gene            1808..2140
                     /locus_tag="VLA60_01585"
     CDS             1808..2140
                     /locus_tag="VLA60_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSF08072.1"
                     /translation="MSIKLYPMKEIKIILEGEHIRFVTDVLDRIKASGYTVFSDISGK
                     GHSGYHKGHLMFNDTSSLQMVLTVVPEDKLEAILSALKPFLERHSGSLFVLDASVLRP
                     DHFLSTDS"
     gene            2828..2974
                     /locus_tag="VLA60_01590"
     CDS             2828..2974
                     /locus_tag="VLA60_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08073.1"
                     /translation="MKNILLLCVSWIFLFIGGCNFEDPYTLNRLGKEQPGSTSGKVLI
                     TIDR"
     gene            2999..3469
                     /locus_tag="VLA60_01595"
                     /pseudo
     CDS             2999..3469
                     /locus_tag="VLA60_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
     gene            3641..4507
                     /locus_tag="VLA60_01600"
     CDS             3641..4507
                     /locus_tag="VLA60_01600"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HSF08074.1"
                     /translation="MKDLGFLHRSYWRGLFRILLLCPLLVSCSTLHITYSTADWILLW
                     KLDRYFALSASQENYLDLQVKAFHVWHRHHQLPQYAQFLTQIDEFSKNELSQAELENI
                     FASVEYFRVHLARRASPPAAEFLATVTPAQIRHFEEMLDQDYRRLVSEIGDDPEVRLE
                     KRVATTSETLTTWVGELSVDQETYIRERMRKIPDTTDMWLAHRKSRQEKLLELLRSAH
                     DPFSLEQGLFQWLADSKTGATEEYLVVSREWREGVQRAVLDIDRILTREQRAHFSRKV
                     QGLIHDIHGLIG"
     gene            4794..5351
                     /locus_tag="VLA60_01605"
     CDS             4794..5351
                     /locus_tag="VLA60_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HSF08075.1"
                     /translation="MTQHVPEWLRAARSKWEYRGQGRPPFAEKPSPGQESVWDYPRPP
                     RLMPDHRRVVVRIREKVLADSCSAFRFLETASPPTFYLPPSDVDVSALVLTCASSLCE
                     WKGTAQYWMLAEGQKEAEPVAWTYPHPYPGFESIAGYFSFYPGRVECYVNDERVLPQP
                     GGFYGGWVTRDIVGPFKGPMGTGEW"
     gene            5666..6541
                     /locus_tag="VLA60_01610"
     CDS             5666..6541
                     /locus_tag="VLA60_01610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSF08076.1"
                     /translation="MTLPTRVLGKTGISLPILGFGTAQAGKRLTHREAIHLFEAALNA
                     GVKYFDTAPEFAGYGKAQEQLGHLLKMRRQEIFLVTKCYDPTYDGALKLLERNLKELQ
                     TDYADLVFVHSLGADKMDPAVVFGRKGAYSGVVKAQSLGLTKFIGLSGHNRPVRFADA
                     IQRVEVDVLLNVVNFADRHTYNFEERVWTVANRLNLGLIAMKVFGGGQKSGGNSLSHC
                     LMPISHLDMAFRYALSVPQVTCAVIGMATPGELKDNLRRARAFTPLNDREGKNLLKAG
                     RVLASQWGTHFGALV"
     gene            complement(7123..>8539)
                     /locus_tag="VLA60_01615"
     CDS             complement(7123..>8539)
                     /locus_tag="VLA60_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HSF08077.1"
                     /translation="AMATRPYEEPVVRHDAVLESIDYDAFQEIVFKRERGLGEDGTVN
                     FPAQFFHLGRYFKIPVKIHALEDGRSREILYRSEYYTFGGTSLGKIFPDDLGFSGFRL
                     QDGPGATTDWMAFLGASYFRSSGELNQYGLSARGVAIDPALPTPEEFPRFTEFWLEPG
                     APDSKEYVIYALLDGPSVTGAFRMVCIKNQQIVMDIDANLYARQDIKRLGVAPLTSMF
                     WYAENNRHQIRDWRPEIHDNDGLAMWTGAGERIWRPLNNPSSVMTNSFVDTNPKGFGL
                     LQRDRAFYHYEDDGVFYDRRPSVWVEPIGEWHEGAIQLIEIPTDDEIHDNIVIYWLPK
                     EPVKAGSEWHYAYRLHWVATEPYPSEAVARVNHTRLGNAGIPGQPRPKGGRKFVIDFE
                     GGPLNEIEKLDKVQPVISTSKGRIDNQYALQIVGTKNWRAFFDLYVEGKEPVNLRLFL
                     QLGDRTLTETWLYQYLPFSYD"
BASE COUNT         2005 a         2101 c         2277 g         2156 t
ORIGIN      
        1 aggcggggtg gccaggcccc cggcccaggc catgttttgc attgatgtgc gttcggaggg
       61 gttccggcgg catcttgaag aagtgggggg aaatgaaacg ttcggatttg ccggattttt
      121 cggccttccc atctgctatc agggattcgg cagtgaacag gaaacggatc aatgtccggt
      181 cctgctcaaa cccaaacatg tggtcaggga aattccccgg gcctctcagg gaaaagcggc
      241 tgaacgattt ctcgaacgtc aggctctggc caagaccgga catgccctgc ttcatgattt
      301 gaaagaaaat gtgattaccc cgtatgtgat ggtcgaggcg atcggctggt ttttcgggtt
      361 cagactcttc ggccagacta ttgctccggc ctggtaccgg acgttcaccg cctggctgaa
      421 ggaatggttt gcgattccga ttgccaccac gttaaccgtg gacaaattgt cccgggagga
      481 ggcggaagaa atagtggcgt ccgaacatcg ggccacaatt tatcggctct taatcgaacg
      541 ctacggccgt ctgggtttgg ccgttccgca cgatcaagtg gatcgacttc gcaggcaggc
      601 cttgtatcag cttgaaatgg atagcacgga ggaagactct ctaggccggt tgttagggtg
      661 gaatgccgtc agccatgaac agtttctggg agagttgcga aaagactctg gcattcatca
      721 ccgggcggtc agccggcgca tgcaccggat tacccagaaa ggttttacga caaatgaaca
      781 ggcctattat gtggaaaccg ctttgcggat tatcgggttt accacaggct ttgccaggct
      841 ggtattgatc tgcgctcatg ggagtgaatc ggagaacaat ccctatgagt cggctctgga
      901 ctgcggggct tgcggcggca acgagggcat ctcgaacgcg cgggcttttg cagccatggc
      961 caataagcct cttgttcggc agcttcttgc ccagaaagac atccatattc caccagacac
     1021 ccattttctc ccagggcaac acaataccac caccgacgaa gtggaattgt acgatttgga
     1081 ggacgttccc gctactcacc ggaaagacct tcagcgcctc caacaggatc tggaggaagc
     1141 gggacaccga aacagtcagg aacgattgag ccgcttaccg gacgagagcg gtacgccgtc
     1201 gttgccaaga agtctcagcc tagccaaacg acgaagttgg gattggtccc aggtgaggcc
     1261 tgaatggggc ttgtcgagac actcgacttt catcatcggc cggcggttat tgacgagggg
     1321 cgtaaacctg gaagggcgaa cctttttaca ttcctatgat tacctgcagg accccaccgg
     1381 gagatatttg gaaattatca tgacggcgcc cctgattgtc ggcaactgga tcaacatgga
     1441 gcattatttt tccaccgtgg atcccgaggt atacggagcc gggagcaagg tgtaccataa
     1501 tgtcgttggc cggatcgggg tgatgtatgg ttcacaaagt gacttgcgca tcggactgcc
     1561 ctggcagacg gtgtatgatg gtcccaagcc ttaccatgaa ccgatgcggc tctttgccat
     1621 tattgaggcc cccttggagc gcatctccgg catcatcgcg aggcaggatc tcttgcagaa
     1681 attaatcgga aatcagtgga tcaatctggt ggccctcgac ccggtcacca tggagttctt
     1741 tctgtaccat tcatccgagg agtggcggat tattctttaa ttccctttta aggaggcgtg
     1801 cgacgttatg agcatcaagc tttaccccat gaaagagata aaaattattc ttgaaggaga
     1861 acatatccgt tttgtcaccg atgtgctgga tcgaatcaag gccagcgggt ataccgtctt
     1921 cagtgatatt tcaggcaaag ggcacagcgg ataccacaag ggtcacctca tgttcaatga
     1981 tacgagcagt ttgcaaatgg tcctgactgt ggttcccgag gataagctgg aggccatcct
     2041 ctcggcgctg aagcccttcc tcgagcggca ttcgggaagc ctgtttgttc tggatgccag
     2101 tgtcttgaga cccgatcatt ttctatcgac tgattcctaa tcccctggtt gatacatatc
     2161 ggaaacacag cttgagtgcg agatcctctc ggggtgacaa aataaaagac gacaatgaat
     2221 gttgttggcc tgaaaggatt gagaaaaaag attgtcccgc ctttgtcctg ccattcctca
     2281 tttgcagggc acaaagcaat tcgtgggaga agttagaaat ttcaaggaga taaaattcat
     2341 gttatttatg ccaggattta ttctcaaaaa attcccataa tcgttcaccc acccattcca
     2401 atactcgaca ttctgtcata ccgttttttt ttatcaacat tgtagttgaa gggttttggg
     2461 gttccttccc tctcgtctgg aaatctggac aaatacttct ttctctccac gcttcctttt
     2521 ttcattttcc ctttttaact aaattaggtt tttccattaa acctgggctc acggctgatc
     2581 tgagagttgg aacaatttcc taccccccgc tctgatcatc gggtaattcg gtcaggtaga
     2641 acgctagtgg tgtgaagcgg ctccgggggc agaatgagct tgtctaagga ttggacagaa
     2701 tgtgttggtt ccttgcccat aagcggaagt agagtaagag aaataaggaa ataaaatagg
     2761 gagaggagtc cccttggtaa atttcggacc ctttctgtgc gtaaaaaaag ccagagagag
     2821 gccaaaaatg aaaaacatac ttctcctctg tgtgagttgg atttttctat tcatcggcgg
     2881 gtgtaatttt gaggatccct ataccctgaa ccgtttgggc aaagaacaac caggttccac
     2941 ttcggggaaa gtgttaatta ccattgatcg atagaaaagg ccacagagcg tctatgattt
     3001 cacccttttt gatatctcga cccagccggt gtctcttgaa cgatttcagg gtaaggttct
     3061 tcttatggtg aatacggcca gtcgctgcgg atatacagca cagttgaggg acttacagac
     3121 actctaccgt cagtatcaat ctcagggctt ggaagtgttg gtctcccctt ccaacgattt
     3181 tggtggacag gaactcgaga ccaatgagga gattgccctc ttcgccacca cacaattcgg
     3241 tgtcgacttt cctctgtttg ccaagacccg tgtgcgagga agtgctcagc accctctcct
     3301 tgcctattta acgacccaag gccctttcga taaacaagtc cggtacaatt tcgaaaagtt
     3361 cctcgtagat cggaccggca aggttattgc ccggtattct tccgatgttg ttcccctttc
     3421 cgaagaaatc cgtgcggaca tcgaacgggc tttggccttt tcggcttaaa gtggctggag
     3481 tgagcccggt aaccgtggca cccttttcct ctttgataat tccttctgtc cgtatttgaa
     3541 atccactgga gagccatatt ctggctgatt agaggtgacc cgtcaggtta aaatggacag
     3601 tgagggtggt catcgaccgg atcttttttt tagggcacct atgaaagatt tgggtttcct
     3661 ccatcggtca tattggcggg ggttgttcag aattctgctt ttgtgccccc tgctggtttc
     3721 ctgtagcact ctccacatca cgtattcgac ggctgattgg atcctcttat ggaaactgga
     3781 taggtatttt gctctttctg cctctcaaga aaactatttg gatctccagg tcaaggcatt
     3841 tcatgtctgg catcgacacc atcaacttcc ccagtacgcc caatttctca ctcaaataga
     3901 tgaattttca aaaaatgaac tgagtcaggc agagctggag aacatctttg cgtcggttga
     3961 atattttcgt gttcatcttg ccaggcgagc ctctccaccg gctgcggaat ttttagccac
     4021 tgtcacgcct gcccaaatac gtcatttcga agaaatgttg gatcaagact atcgacggct
     4081 tgtgtccgaa atcggagacg acccggaggt gcgtttggaa aaacgcgtgg caaccacatc
     4141 ggaaaccctg accacatggg tgggagagtt gtcggtggac caagagacat atattcgtga
     4201 gagaatgagg aaaatccccg ataccacgga tatgtggctg gcccatcgca aaagccggca
     4261 ggaaaaattg cttgaacttc ttcgttccgc tcatgatcct tttagcttgg aacaaggtct
     4321 ctttcagtgg ctggccgact ccaagaccgg tgccactgag gaatatctcg tggtctcaag
     4381 ggaatggcgt gaaggagttc aacgggcagt cttggatatt gaccggattc tgactcggga
     4441 gcagcgggca catttctcca ggaaggtgca agggttgatc cacgatatac atggattgat
     4501 cgggtagcca acatcaaaca attttgtggt tgattgacca gcccgcctgc aagtcatgaa
     4561 tgatttttat gaaattgagt gagccagtgg cgttgagata ttccaatcac agagggtctt
     4621 tcgagcaacg tatggctccc atgtggtaca aaattcttct cacagttttg ggctgtcttc
     4681 tcctcggtga aatgttgatt atcctatggg catcttgggc cattttgacc tttgaataaa
     4741 catatgtgga tgattatgcc cccatcactt ttccggaaaa ccttgggaat ttcatgaccc
     4801 aacatgtgcc tgaatggcta cgggcggctc gttccaaatg ggaatatcgg ggacagggaa
     4861 gaccgccatt tgcggaaaag ccgtctcctg gccaagaatc tgtttgggat tatccgaggc
     4921 ctcctcgtct gatgcctgat catcgccgag tggtggtccg tattcgggag aaggttctgg
     4981 ccgattcctg ctccgcattt cgatttttgg agacggcgag tcctccgacc ttttatcttc
     5041 ctccctcaga tgtggatgtg tccgctctgg ttctcacgtg cgcgtcatct ctctgtgaat
     5101 ggaaaggaac cgctcagtat tggatgctgg ctgaagggca gaaagaggcg gaacccgttg
     5161 cctggacgta tcctcatcct tatccgggat ttgaatccat cgccggatat ttttccttct
     5221 accccggccg cgtggaatgc tacgtcaatg atgagcgtgt gctgccccaa ccgggcgggt
     5281 tttatggcgg ctgggtgacc agagacattg ttggtccgtt taaaggtcca atgggtacgg
     5341 gggaatggta ggggcggcac gtttccataa caaattttct cgacagagtt gaccgaacag
     5401 gcaaaagaat ttgaaaattt agagaagagt cggtcattgg ttcagaataa gtgtaaaagg
     5461 ggagtgttga ataataataa tttccaaggg catattgacc cacaggcacg ttgcgtatta
     5521 gaggcctcgg ggctgttcaa aaaaattgtc cagcaaggcc gcagtcattt ttgcgcggag
     5581 agcgtacttc cagtacgtga gcacggaaaa atggcgagaa cgccgctggc ggcttttttc
     5641 aacagcccca aaaggaaacc tgagcatgac cctgccgaca cgcgtgttgg gaaaaaccgg
     5701 aatatcctta cccattcttg gatttggcac tgcgcaagca ggaaaacggc tgactcaccg
     5761 cgaggcgata catttatttg aggccgccct caatgcgggc gtgaaatatt ttgataccgc
     5821 acctgaattt gcgggatatg ggaaagccca ggaacaattg ggccacctgc tgaagatgcg
     5881 gcgacaagag atttttttag tcaccaaatg ttatgacccc acctatgatg gcgcactaaa
     5941 gctattagag cgcaatctta aagaactcca aacggattac gcggatttgg tctttgtgca
     6001 tagtctcggg gctgacaaaa tggatcccgc tgtggtgttt ggtcgcaagg gagcttacag
     6061 cggggtggtc aaagcccaat cattgggatt aaccaaattc atcggcttgt ccggtcacaa
     6121 tcgtcctgta aggtttgccg atgccattca acgcgttgag gtcgatgtgc tgctcaatgt
     6181 cgtcaatttt gctgaccgac atacctacaa ttttgaagag cgagtgtgga cggttgccaa
     6241 ccgcttgaac ctgggcctaa ttgccatgaa agtgtttgga ggggggcaga aatcaggggg
     6301 aaacagccta agccattgct tgatgcccat ctcccattta gatatggctt ttcgctatgc
     6361 cttgagcgtg ccccaggtca cgtgtgcggt catcggaatg gccacaccag gggaattgaa
     6421 ggacaacctt cgacgcgcgc gcgcatttac acctctgaat gaccgggaag ggaagaatct
     6481 tctgaaggcc ggccgcgttc tcgcttctca gtggggaacc catttcggag cgttggtcta
     6541 atccttcatc tctttagtta gaggtcgaga ggaacgcaag gaattctgag tcccatcgtt
     6601 ttctctcaaa atttaatcca acgaaaaaga ttttttaagg ttacggtgag gtttcggggt
     6661 agcggacagg tgatcccatg tctccttccc tctcaaatca cacctcgcga accagttgag
     6721 atgcgtgatt ctccctgtac attcggaacc cctcgccaca tttattttta caaggcaggc
     6781 gaacccgttt gtgtaggtac gttttgatcc cacacactct catggtgtgg ctagggctac
     6841 gtgggatatt ttttggtaga aaaaagacct cccgcaggaa acttctagaa aaactttacc
     6901 tgctgggagt gttaaataaa accaatttcc aagggcatat ttacccacag gcacgttgca
     6961 tattagaggc ctcggggcgg ttcaaaaaag ttcgtccagt cctgctctga gtcacgtcga
     7021 agggaaggcc gcagtcattt ttgtgcgcag agcgtacttc cagtacgtga gcacggaaaa
     7081 atggcgagaa cgccgctggc ggattttttc aagagccccc tgctagtcat agctaaaggg
     7141 caggtattgg tagagccagg tttcggtcag ggttcgatct ccaagttgga ggaaaaggcg
     7201 gagattgacc ggctccttgc cttcgacata gagatcgaaa aacgctcgcc agttcttggt
     7261 gccgactatc tgcagcgcgt attggttatc gattcgtccc ttggacgtgc tgatgaccgg
     7321 ttgaaccttg tccagctttt cgatctcgtt gagcggccca ccctcgaagt cgattacgaa
     7381 ctttcgaccg cctttcgggc gcggctgccc gggaatgccg gcattgccga gtcgggtatg
     7441 gttgacccgt gcgactgctt cggacggata gggctcggtt gccacccaat ggagccgata
     7501 ggcgtagtgc cactcggagc cggccttcac tggttccttc ggcaaccagt agatgacgat
     7561 attgtcatgg atttcgtcgt cggtcggaat ttcgatcaat tggattgcgc cttcgtgcca
     7621 ctcacctatc ggctcgaccc agacgctcgg tctccggtcg tagaatacac cgtcatcctc
     7681 atagtggtag aaggcgcgat ccctctgcaa tagcccaaaa cccttggggt tcgtgtcgac
     7741 aaagctgtta gtcatgaccg atgacgggtt gttgagtggg cgccaaatac gttcgcctgc
     7801 gcccgtccac atcgccagac cgtcgttgtc atgaatttcc ggccgccagt ccctaatctg
     7861 atggcgattg ttctcggcat accagaacat gctggtcagc ggcgccaccc ccaggcgttt
     7921 gatgtcctgg cgtgcataga gattggcgtc gatatccata acaatctgct ggttcttaat
     7981 gcagaccatg cggaaagcac cagtcacact cggcccgtcc agcagggcat agatcacata
     8041 ttccttgctg tccggggctc caggctccaa ccagaattcg gtgaatcgtg gaaattcctc
     8101 gggggtcggc agggccgggt cgatcgccac ccccctcgcc gaaaggccgt attgattgag
     8161 ctccccggag ctacggaaat aggaggctcc gagaaacgcc atccaatcgg tggtcgcgcc
     8221 tggtccatcc tggaggcgaa agccggagaa gccaagatca tccgggaaga tcttgccgag
     8281 actggttccg ccgaaggtgt agtactcgga acggtaaaga atttctctgg accggccatc
     8341 ctcaagggca tgaatcttta ccgggatttt gaaatagcgg ccgagatgga agaattgagc
     8401 cggaaaattc acggtaccgt cctcgcccaa cccccgctcc cgtttgaata cgatttcctg
     8461 aaaggcgtcg tagtcgattg attccaagac tgcgtcatga cggaccaccg gttcctcata
     8521 gggcctggtg gccatcgct
//
LOCUS       DATIAL010000048         2533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_761887, whole genome shotgun sequence.
ACCESSION   DATIAL010000048 DATIAL010000000
VERSION     DATIAL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761887"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VLA60_01620"
     CDS             <1..752
                     /locus_tag="VLA60_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF08078.1"
                     /translation="DEGTLIFQPRNCDTAPGVFLPLTYVRAWDPHAIVVLLPADHFIC
                     PEDRFVAAVRRAVRAVEFLSDQMILMGVRPSHLELDYGWIAVGGALGWSGGAAIRRIQ
                     SFVEKPEPVDGKRMMSEGALWNTLVLVTKVETLWKSGWLCFPDVMERFEGLRRQIGTP
                     AEGSTLQAMYQDMPTRNFSRDVLQPLAGQLGVMELNDVTWSDWGRPERIVETLRSMGK
                     KPAFPMEAFIGSGAPSIPQIPRDMGYPYLSC"
     gene            1293..1760
                     /locus_tag="VLA60_01625"
     CDS             1293..1760
                     /locus_tag="VLA60_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HSF08079.1"
                     /translation="MEKPGDPERIIWEGYPSWGQFTWLYFFSLWTGLRGLFLARLGFP
                     GWEMWLVGAGLLLGLVVILRYWAKYLLTSKRVILRNGYSGKDMASVEFEKFKSVEVMQ
                     GPVSRFLGIGTLVVQCEDSGRLRFRGIKDPEVIETKLRALLSPSSPVLTHQPA"
     gene            1805..2392
                     /locus_tag="VLA60_01630"
     CDS             1805..2392
                     /locus_tag="VLA60_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08080.1"
                     /translation="MIPSLSRRTYLVLGLTFLTVAGALFLEYLLSVSADRPFGHTQVA
                     HLVGWTGLVFIGLTFVYPLIRWMYPNEAWSRGWFQVHIVFGIVGPLVIFLHAGVHFHA
                     LVPIIALIAMVLVVMSGITGQALHYLAFQALYERRHEWAFEGMTEDAIEAHLHGLALQ
                     EETLRWWKCLHGPLTWSFVTFTLLHIGGALFFGGF"
BASE COUNT          506 a          595 c          697 g          735 t
ORIGIN      
        1 aagatgaagg cacgttgatc tttcagcccc gaaattgtga cacggctcct ggagtgtttc
       61 ttcctcttac ctatgtaaga gcctgggatc ctcatgcgat cgtggtcctc ttgcctgccg
      121 atcattttat ctgtcccgaa gatcgctttg tggccgctgt gcgtcgggcc gtgcgagcgg
      181 tagaattttt atctgaccaa atgattctca tgggcgttcg tccttctcat ctggagcttg
      241 attatggatg gatagctgtc ggaggagcac tcggatggag tggaggggcc gctatccggc
      301 gcattcaatc gtttgtagaa aaaccggagc ccgttgacgg caagcgaatg atgtcggagg
      361 gagcgttatg gaacaccctg gtgctggtga ccaaggtcga gacgctgtgg aaatccggat
      421 ggttgtgctt tccggatgtg atggagcggt ttgaagggtt acgaaggcag atcggcacgc
      481 cggccgaagg gtcgaccctc caggctatgt atcaggatat gcctacgcga aatttttccc
      541 gtgatgtcct gcaacctctt gccggtcaat taggtgtcat ggagcttaat gatgtcactt
      601 ggagcgactg gggccgtccg gaacgaattg tggagacctt gcgatcaatg gggaaaaagc
      661 cggcttttcc aatggaggcc ttcataggat ccggtgcgcc atccattccc caaataccaa
      721 gggacatggg ttatccctat ctgagctgct gaagaaattc ttcttggctg tctagaggaa
      781 ttttctaatc taagcagccg taccagcgag taatcattca gcgcagtcca tcccaacagg
      841 atggacgagg gctccgtttc acactgttct cccatatctt tgtatggccc ttttccctcg
      901 ggattatttc tcttttcccc gactcatcct ttttcaccca aaattttcct cttttttctc
      961 ccattcaggt ctattggtct ggtcattgtg gcccattttc ttttctgctt tccattaccc
     1021 agtgtcgcag aaatcaacag attgactctc tgggggtggt gcgtttgtca tcaaaggcct
     1081 aatctcagac gggattcatt tctctctttt cctttaaatc gcttcgcggc cgtatagggt
     1141 ctactggcac agaggtcgca agagagacct tatgtgtgag aaagctcatg atatggcgcg
     1201 ttggcgtgtt tcggggaatg agatctgtgt tttgagtctt ttttcggaag atgaaacatc
     1261 attccaggtg ttaaacacaa aggatgaagg atatggaaaa gccaggcgat cccgaacgga
     1321 ttatatggga gggctatccc tcgtggggac aatttacgtg gctatatttt tttagccttt
     1381 ggacaggatt gcgaggtctc tttcttgcgc gattaggctt tcctggctgg gagatgtggc
     1441 ttgtcggggc agggctgttg ttggggctcg tggtgattct tcgatattgg gccaaatatc
     1501 tcctcacgtc gaaacgagtg attttgagga acgggtattc gggaaaggat atggccagcg
     1561 tggaattcga gaagttcaaa tctgtcgaag tcatgcaagg gccagtatcc aggtttttgg
     1621 gaatcggtac ccttgtggtt caatgcgagg attccggccg tcttcggttc cgtggcatca
     1681 aggaccctga agtcattgaa acaaaattac gggcgcttct gtccccttct tctcccgtgt
     1741 tgactcacca acctgcctga acgatgaagg cacctgctgc gatgaggaat gagaattatt
     1801 ctttatgatc ccgtctcttt cgcgtcgtac atatttggta ctcgggttga cttttctaac
     1861 cgttgccggt gccctgtttc tggaatacct tttgagtgtc agcgcagaca ggcccttcgg
     1921 tcatacgcaa gtggctcacc tggttgggtg gaccggcctg gtattcattg ggctgacctt
     1981 tgtgtatcca ctgatacggt ggatgtatcc taatgaggcc tggtcacgcg gatggtttca
     2041 ggtacacatc gtctttggga ttgttggtcc cttagttatt tttctccatg ccggtgtgca
     2101 ttttcacgcc ctggtgccga tcattgccct tatcgccatg gtcctggtgg tgatgagcgg
     2161 gatcaccgga caggctttgc attatctggc gtttcaagca ttgtatgaac gacggcatga
     2221 gtgggctttt gaagggatga ccgaggatgc gattgaggcg catttgcatg gtctcgcatt
     2281 acaagaagag acgctccgat ggtggaaatg tctccacggc cccttaacgt ggtcatttgt
     2341 cactttcacg ctgctgcaca tcggaggagc cctctttttc ggcggcttct aaatcatggg
     2401 gtgttatcga ccatgggttg cgccccttat ctgggttctg acaggtctga tcatcgtggc
     2461 tctggcctgg acttctccct tatacactga agccttgtgg gctcctggag atcttagccg
     2521 gtatcacact gac
//
LOCUS       DATIAL010000049         3903 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263 k141_9647,
            whole genome shotgun sequence.
ACCESSION   DATIAL010000049 DATIAL010000000
VERSION     DATIAL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9647"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2587
                     /locus_tag="VLA60_01635"
     CDS             <1..2587
                     /locus_tag="VLA60_01635"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HSF08081.1"
                     /translation="FDLPEDSRKSIIAQTIHECEQCRHDPEGAYLNNLWKSSLSAFQL
                     FDPLSGNSTLKVRDTTLDSVSLEEESHVVPVDLPPDRTLGDWLDSLTGGTLVEIINNQ
                     LIKWIAAFVDEGIAGWGMPVRERGFYSAWRELAQEDYSGGFLGIPHIRQRIRELSDDP
                     EESLSHHLHVLQIPRERWGEYLSRHLAQLPGWAGFIRWRGDNPGYPAQQLHPIDPMQY
                     LAVRLFYESEMVDGFCRREWGIAGTRSALSVYWEEHLEQYQALITPQAHASDLNSQGV
                     CHKAWRLFHLAQFLELTPSDLHVLAHDEISTLLEWLDTFPRNAHGPVWLEAYEDVYRQ
                     DFLKKLAGHQGTVRILDGRPRAQTMFCIDARSESFRRHLEDRGPYETFGYAGFFGVPM
                     SYMAFDSHDHLALCPILLTPKAEVVEIPRGKQDDRICDYFSGSRWHQFSHHLFHDLKQ
                     NPIASLIHIDVLGIFFSIGLIGKTLFRSSFNSLKNLLHNWFANPLTTCIPVDRVIEQE
                     ITARRPGQLTKGFTLHEQADFVEGGLRVIGLTKNFGRFVMVCGHGSQSDNNPYFSALD
                     CGACGGSHGDPNARVFSAMANNPEVRRILKEYDLVIPEDTWFLPAKHNTTTDQVTLYD
                     LVDVPPAYVEELELFIKDLEQAGAHQALERCHRIPGAPTEISPHAAFLHVRTRSMDWA
                     NSRPEWGLSGNAAFLIGRRAITKGLCLEGRVFLHSYDPESDPDGGFLEKIMTAPLIVG
                     ELISLTYYFSSVDPWCYGSGSKVIHNIVAGIGVMLGSQSDLQKGLPLQSVNDGTHHYH
                     EPMRLLAIIEAPPDRIQAIIQKHTLLQHIFHNQWMNLMALDPDSKNFSRYLTDSTWEA
                     VTVNS"
     gene            3219..3482
                     /locus_tag="VLA60_01640"
     CDS             3219..3482
                     /locus_tag="VLA60_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08082.1"
                     /translation="MSRHNSSPYRENPDRISHAYLTYDHTNFVLVMVKAALVLTLVGR
                     IESLLKVIKASSLHRITIESEIRSIGFVEQGYGCKIPGFSNYE"
     gene            3612..>3903
                     /locus_tag="VLA60_01645"
     CDS             3612..>3903
                     /locus_tag="VLA60_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HSF08083.1"
                     /translation="MQPSYPRQFIANTLALFVVWLVLSGRYNLSHMILGFIVSCGVAW
                     LNTGYPHSPFHNFPLGRQMRYWPWLFLRIVESSWHLTKIILDPALPIKPKLLT"
BASE COUNT          937 a          897 c          981 g         1088 t
ORIGIN      
        1 attcgacctt ccggaagatt cccggaaatc gatcattgct caaacgattc atgaatgcga
       61 gcaatgccgg catgatccgg aaggtgccta cctgaacaat ctctggaaaa gttcattatc
      121 ggcatttcaa ttgtttgacc ccctatcggg gaattctact ttgaaggtac gggatactac
      181 tctggattct gtgtccttgg aagaagagtc tcacgttgta ccagttgatt tgcccccaga
      241 ccggacgctg ggtgattggc tggacagttt aacggggggt acactggttg aaatcatcaa
      301 taaccaactg atcaaatgga ttgctgcgtt cgttgacgag gggatagcag gatggggtat
      361 gcctgtaaga gaaaggggat tctattcggc gtggcgagaa ctggctcagg aggattattc
      421 aggcggattt ctgggtattc ctcatattcg acaaaggatt cgtgaacttt ccgatgatcc
      481 tgaagaatca ttgtcccatc accttcatgt tcttcaaatc ccccgggaac gctggggcga
      541 atatctctcc cggcatctgg ctcaattgcc tggatgggcc gggtttatcc gctggcgcgg
      601 tgataatccc ggatatcccg cgcaacaact ccatcccata gatcccatgc aatacctcgc
      661 cgtccggctc ttttatgaat cggaaatggt ggacggattc tgccgacggg aatggggaat
      721 tgcggggact cgctccgccc taagcgttta ttgggaagaa cacttagagc aatatcaggc
      781 tttgataacc cctcaggctc atgcttcgga cctcaattct caaggcgtgt gccataaggc
      841 ctggcggctc tttcaccttg cccagttttt ggagttgact cctagtgatc tgcacgtatt
      901 ggctcatgac gagatttcga ctttgctgga atggctggat acttttccac gcaatgctca
      961 tggcccggtg tggttagagg cctacgagga cgtctatcgg caagacttcc tgaagaagct
     1021 cgcaggtcat caaggaactg tacggatttt ggacgggcgg cctcgagcac aaacgatgtt
     1081 ttgtatcgat gcgcgttcag agtcgttccg tcgacacctt gaagaccgag ggccttatga
     1141 aacattcggg tatgctggat ttttcggagt gccgatgagt tatatggcct ttgatagcca
     1201 tgatcacctg gcattatgcc caattttatt gaccccaaag gcggaagtgg tcgaaattcc
     1261 ccgaggcaag caggatgaca ggatatgtga ttatttctct gggtcccgat ggcaccagtt
     1321 cagccatcat ctatttcatg atctaaaaca gaatcctatt gcctccttga tccacattga
     1381 tgttctaggc atatttttta gcatcggact gataggaaaa acactgtttc gctcttcctt
     1441 caactcattg aaaaatctac tccataattg gtttgcaaat ccactgacta cctgtattcc
     1501 tgttgatcgt gtcattgagc aggaaatcac ggcacgtaga cctggccaat tgacaaaagg
     1561 ttttaccctt catgagcagg ccgactttgt ggaaggcgga ttgcgtgtga tcgggctcac
     1621 gaaaaacttt ggcaggtttg tcatggtgtg tggtcatgga agccaatcag acaataatcc
     1681 ctatttttca gcccttgatt gtggagcttg cgggggaagt cacggtgacc cgaatgctcg
     1741 ggtcttttcg gccatggcca ataatcctga agtccggcga atcttgaaag agtacgacct
     1801 tgtcatcccg gaggacacct ggtttcttcc tgccaaacat aacacgacca ccgatcaagt
     1861 gacgctgtat gaccttgtcg atgttcctcc tgcatatgtt gaggagctag aattgttcat
     1921 aaaagatttg gaacaggctg gtgctcacca agccttagaa cgttgtcatc ggattcctgg
     1981 tgccccgaca gaaatctctc cacacgcagc cttcttacat gtcagaacac gaagcatgga
     2041 ttgggctaat tcccgtcccg aatggggact gtcagggaac gccgcctttt taattggaag
     2101 acgggccatc acaaaaggac tgtgtttaga ggggagggtc tttttgcatt cgtacgaccc
     2161 tgaatcggat ccggatggag gctttcttga aaaaattatg accgcacctc tcattgtagg
     2221 cgaattgatt agtttaacct attatttttc ttcggtggat ccgtggtgtt acggtagcgg
     2281 gagcaaggtc attcacaata ttgtcgcggg aataggcgtc atgttgggga gtcagagtga
     2341 tctgcaaaaa ggccttcccc tccaatcggt aaatgatggg acacatcatt accatgaacc
     2401 gatgcgctta ttagccatca tcgaagcacc gccggatcga atacaggcca tcattcagaa
     2461 acacactctt ctccagcaca tatttcataa ccaatggatg aatctgatgg cgttagatcc
     2521 tgactccaaa aatttttcac gatatctcac ggattcaacc tgggaggcag ttacagtcaa
     2581 ttcgtgatgc tatgatgggt gagagttgaa tttgaaagag cgtaatttct cccatttctc
     2641 tggagtagag gattataggg cttttggttc catctgatgg ccaagtagcc ccagatagga
     2701 tgtgtatagg aggaccccat taaaattttt ccactcgctt cagggttgtg tatagaagag
     2761 gtgcttggta taaggtcgga ggtttccgta ttttgaaaaa gatgaggcca cttttttatc
     2821 ggtcgaagag tcgggtagta gagaaagtgg gagcatcgga ttcagcaaca aaacatcata
     2881 gataacggcc gtagggtttc cagttttccg gtaatttccg gataggctag aaacagtaca
     2941 agccaaccgg gtttttggag cacctcattc gaaaaacctc tggttggctt ttttttacct
     3001 tgcgggggct gaggaggaag taagcctcct ctccagtttg ggggtttagc acaacacggg
     3061 aggcagggcg atgctttcct ggtagttttt ggattcatgg tttcctgtgg agcggtatgg
     3121 cggaacaatg gatattcctt ttctcctttt cacaacctta cgtgggacta tccggtatta
     3181 tagaccccct ggctctttct tcgaacgatc gatacctgat gtctcgtcac aactcttctc
     3241 cctatcggga aaatcccgac aggatttccc atgcttattt aacctacgat cataccaatt
     3301 tcgttttagt catggtgaaa gcagccttag tgctgacctt ggtgggaagg attgaaagtc
     3361 tattgaaagt gataaaagct tcttccctgc atcggataac gatcgagtcg gagatccgga
     3421 gcatcggatt tgtcgagcag ggttatggct gtaagattcc aggtttcagt aattacgaat
     3481 aatttggatg caggaaaagt caaccaggtt cctgaaatat cccccttcga aaaacctccg
     3541 gttggcttgt tttatttagt gggacggact gaaaaaggac cggtaggttt tccaatttga
     3601 aagttgacac gatgcagcct tcttatccga gacagtttat cgccaacact ctggcacttt
     3661 ttgtcgtttg gctcgtgtta tccggccggt acaatctttc tcacatgatc ttgggtttta
     3721 tcgtctcctg tggggtggcg tggttgaaca ccggctatcc ccactctccc tttcacaatt
     3781 tccccttggg gcgtcagatg aggtattggc cctggctctt ccttcggatt gtcgagagca
     3841 gttggcattt gaccaaaatc attctggacc ccgccttacc cattaagccg aagctcttga
     3901 ctt
//
LOCUS       DATIAL010000050        11203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1515208, whole genome shotgun sequence.
ACCESSION   DATIAL010000050 DATIAL010000000
VERSION     DATIAL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515208"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VLA60_01650"
     CDS             complement(<1..473)
                     /locus_tag="VLA60_01650"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF08084.1"
                     /translation="MSGSEHEQIARLRAFLNQQVIGQEGLTLRLLIALMADGHLLVEG
                     APGLAKTTAIKALASGIEADFHRIQFTPDLLPADITGTDIYRPHDGSFRFQPGPIFHN
                     LVLADEINRAPAKVQAALLEAMGEHQVTIGRTSYPLPELFLVMATQNPIEQEGTYP"
     gene            1549..1857
                     /locus_tag="VLA60_01655"
     CDS             1549..1857
                     /locus_tag="VLA60_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08085.1"
                     /translation="MNRKLCAINVIGCVLLGSTLALGNPSMLPDHPGYPMGDAKSPVT
                     GQPVANDPGKSPPSQEESLQQAAGFHDAHAMNPGKEERPNIVSPGALKSMDSKSKSTK
                     "
     gene            complement(2009..2161)
                     /locus_tag="VLA60_01660"
     CDS             complement(2009..2161)
                     /locus_tag="VLA60_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HSF08086.1"
                     /translation="MTGFLGELWDFMRERKKFWLAPIIIMLLLLGGLIILSEGSAVAP
                     FIYTLF"
     gene            complement(2175..2579)
                     /locus_tag="VLA60_01665"
     CDS             complement(2175..2579)
                     /locus_tag="VLA60_01665"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HSF08087.1"
                     /translation="MTSTIHQPTAKDLRSFGLLMGGVFLIVAVWPLVIHGESLRIWAS
                     VIAAVFGTMGMVFPKGLGPLHRVWMKIGEKLGWINSRIILSLLFFGMFTPMAFIMGLL
                     GKRPLQLGYDPKANSYRVVKKARAADHVLKPF"
     gene            complement(2598..4466)
                     /locus_tag="VLA60_01670"
     CDS             complement(2598..4466)
                     /locus_tag="VLA60_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HSF08088.1"
                     /translation="MTKPTAKLTSILGVSAYYHDSAACLIQNGKIVAAAQEERFTRKK
                     HDAGFPSHAVEYCLRQGGVGMRDVDYLVFYDKPFVKFERLLETYLSYAPKGLGSFLTA
                     IPVWIKEKLFLRNLLEKAFRQVGSLEKKENLPPLLFGEHHESHAAAAFFPSPYEEAVV
                     LCMDGVGEWATTSAWIGRGNQLTPLWEIPFPHSIGLLYSAFTYYTGFKVNSGEYKVMG
                     LAPYGEPKYVKTILDNVVDVKPDGTFRLNMDYFDYCTGLRMTNNKFDAIFGGPPRQAE
                     STLTQREMDLARSVQEVTEMVMLRLANSLHRETGLSNLCLSGGVALNCVGNGRVLREG
                     PFSGLWIQPAAGDAGSALGAALNVHYIYLNQPRVCQGPSDSMRGSYLGPRFSNEEIES
                     RLQQLEARYERLEDSDLYRRVAEYLAEGKVIGWLQGHMEFGPRALGGRSILGDPRSEK
                     MQSVMNLKIKYRESFRPFAPSVLRERVSEYFELDADSPYMLLVAPVAEKRRRAVQPDQ
                     QKLFGIDLLNVPKSDIPAVTHVDYSARVQTVSPDTNPRYYQLLQEFEKQTGCAVLINT
                     SFNVRGEPIVCTPEDAYRCFMRTEMDVLVLENCLVLKDHQKPVQKDETWMKEFELD"
     gene            complement(4619..5410)
                     /locus_tag="VLA60_01675"
     CDS             complement(4619..5410)
                     /locus_tag="VLA60_01675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HSF08089.1"
                     /translation="MNYFHRSGLCLLMIMCAWSSQVEARVQIKTIPYNHGEVVLEGVV
                     AWDDEIKGKRPGILVVHEWWGLNEYARTRAEQLAALGYVAVAVDMYGKGKVTTHPDEA
                     RQWMQQTTANVKMWQARAKEGLRLLQTNPLVDQTRLAAIGYCFGGATVMQMVYDGAPV
                     KGVVSFHGSLPLPSASSAIKSAVKILIAHGEADPFLSQDHITKFKHALDEASLDWHMV
                     IFGGAKHGFTNPSANQYGMNGVQYQEQADRRSWEHMKLFFDELFR"
     gene            complement(5576..7294)
                     /locus_tag="VLA60_01680"
     CDS             complement(5576..7294)
                     /locus_tag="VLA60_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSF08090.1"
                     /translation="MNTSWIAFLERPAIHISLLLVIGFLAFSSNASISLFGETEGLYA
                     IITHTMMAAEDYVHLWLRGEPYFSKPPLFFWLQAGFIHGLGWSEAALRLPSILSSLGT
                     MITTYYLGRLLFSEMAGFWSALVCATCYAGLWFGPLAIIDPVLTFCMTLGMYAWARAY
                     FQKSSQWWYLIGFVALALGSMVKTLHAMALPVLVMGIFLWMRRDGRVFREWYFWVGAL
                     LSGLLLGAYYLLLGPEFRQHFFFEENLKRMISVSGDQQYSAWEAYWGKRPIFWYGLVI
                     WFDFFPWSALLPFGLFLIWKHRPWLESPKELWVFLWVVVYFVAMSLGPEKHERYLIPL
                     LPAFGLLVGFVYHRLLHEAPVSEGKGLLRGLLACLGGIFVVSVFLGPILLQKKWHVPL
                     DIIPLALRVGLGMLGLIMIGLAVRNYLRMGLVGMGVLGVALMVTVTGFIIPGIQAEGS
                     PRLAFDENQRRLNKQTDPILVFQSWDWRGDEDEFYWDYLHGRSRIVGKGLKDSLALEE
                     LKRDVQQSTRLVMMTKEQYQQIIFGAPDLSANVLFEFYRSKKDIVLLSLGWRAQLADS
                     WETQKP"
     gene            complement(7642..7718)
                     /locus_tag="VLA60_01685"
     tRNA            complement(7642..7718)
                     /locus_tag="VLA60_01685"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7682..7684),aa:Arg,seq:tcg)
     gene            7951..8262
                     /locus_tag="VLA60_01690"
     CDS             7951..8262
                     /locus_tag="VLA60_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08091.1"
                     /translation="MIRTEQLASDTIMFHVQGPFNKQVAKKLGLLVFRSYHLGCKTFL
                     CNLSLAKAFDKDAYSQLTLIGEGLRDKGRNWRVISPPSSNGNQLILRTSLQFLVPANL
                     N"
     gene            complement(8604..9044)
                     /locus_tag="VLA60_01695"
     CDS             complement(8604..9044)
                     /locus_tag="VLA60_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08092.1"
                     /translation="MKKEAKLEPGQVVDTLGELIASLSAFAAKVPAKSMLALSGGIRP
                     TPEAVDAYETTVYRFRDRVGVTYKTLPPLFVESLEAFEAGKVFDAVPPLLQCVEQLVE
                     LHNQETITFTPPQQQRLRDYHRRLEKLVPEATQSEVDLPTPESY"
     gene            complement(9079..10125)
                     /locus_tag="VLA60_01700"
     CDS             complement(9079..10125)
                     /locus_tag="VLA60_01700"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HSF08093.1"
                     /translation="MPVATANRAVPVLYKVIDRPVFLTAMPHVATRTEFIVTAGESPK
                     RLDHFLANREPYFSRTALQRLIEDGHITVGGQVVKPSHKVRPGDCVVLVVPRPEPVAI
                     NPEPIPLVILYEDECLLVLNKPAGLVVHPAPGHWTGTLVNALLHHMQSGEGHLSTIGG
                     KERPGLVHRLDKETTGILVVAKNDQAHRLLAGQFKAHSIIRVYEAFVWEGPKTREGRI
                     ELSIGRDTKDRKTFSARTTNPKASLTSYRVKERFGAAAAHVELFPGTGRTHQLRVHLK
                     AIGCPILGDQTYGGKKVMVVGGIGIPRVMLHAKVLGFIHPVSNKMVTFSAELPEDMVT
                     VLNALKGNEDEWSS"
     gene            complement(10082..10537)
                     /locus_tag="VLA60_01705"
     CDS             complement(10082..10537)
                     /locus_tag="VLA60_01705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HSF08094.1"
                     /translation="MAGAGVGTSSEKILLEVVTPDHRLLSKEVDYVSAPGSEGDFGVL
                     PGHCHFLTTLRIGELQYRIGEQTEYMAVLWGFAEVTPTKVTILAEIAEKAEDINVERA
                     EDAVRKAEERLERGGLPSEVEEARVSLEKARLRQKIAGRHRQQGGSRLI"
     gene            complement(10537..>11203)
                     /locus_tag="VLA60_01710"
     CDS             complement(10537..>11203)
                     /locus_tag="VLA60_01710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSF08095.1"
                     /translation="NIFRFTQAGSEVSALLGRMPSAVGYQPTLGTEMGTLQERITSTK
                     KGSITSVQAIYVPADDLTDPAPATAFAHLDATTVLSRQLAELGIYPAVDPLDSTSRIL
                     DPHILGEEHYQVVQRVQGTLQRYKDLQDIIAILGMDELSEDDKQTVARARKLQRFLSQ
                     PFHVAEAFTGSPGKYVKLVDTVRSFKEIVDGKYDDLPEQAFYMVGPIEEAIEKAEKLG
                     YKR"
BASE COUNT         3028 a         2914 c         2471 g         2790 t
ORIGIN      
        1 ggatacgttc cttcctgctc aatggggttt tgggtggcca tgactaaaaa caactcgggc
       61 aagggataac tggttcgccc gatcgtcact tgatgttccc ccatggcctc taatagggcg
      121 gcctgcacct tagcaggggc ccgattgatt tcatcggcca ggacaagatt gtgaaaaatc
      181 ggtcccggtt gaaatcgaaa agagccgtcg tggggacgat aaatgtcggt cccggtaatg
      241 tcagcgggga gtaaatccgg ggtaaactga atccggtgaa aatcagcctc aatcccggag
      301 gccaaggctt taatagcggt agttttggct aaacctggcg caccttcaac gagaaggtgt
      361 ccatcagcca ttaaggcaat caagagcctg agggtaagac cctcttgtcc aatcacctgt
      421 tgattcaaaa aggctcgaag ccttgctatc tgctcgtgtt cagatccaga cataggtctt
      481 gatatgaacc cggggggttc ccatagtcaa agtattactg attccccttc ttcgatggaa
      541 atggggcaat ctcaatttgt tatgctgcaa atcctgagag cctctttttt ataataattt
      601 accatttttg agagtcaagg ggttttgcgg gaaaatagta ccaatgaacc ccaaaaccct
      661 taaccttctc aatggcccct tgaaagtcag atggatgagg gccgcgagag ggatcccaac
      721 ttcgacaata ctccgcataa tggggtgccg tgtgcaatga taaggatatg ttccggggaa
      781 taatcgttat ttcaaatgtt tcatgcagac gacaactgaa tcaaatactt atactccacc
      841 ctctttgcag ccgttatgcg tattcgtatg agggtagccg tgggattctc ccataaatac
      901 ggattcctga ggtcgcgaaa gcctgttgag gagtaaaata cggcgcaaat cttttaatta
      961 agattgaata gggcttggaa cttctccaag ccatgaagtg cttattcaca agcatcaaac
     1021 ttttttcatt tataaactgg ggatgtttca tattctcttc tggtaatttc attgttccta
     1081 tgaattcgat ctttttgtga tcgccatgcc tttcatttgg tattttttta atctttgggt
     1141 ttttcatttc aaaaactatc gcaaaattca ccctttacca ggatagtgtt tatctgcctc
     1201 actcgacaga cttgccccaa tttgcaccac ttgcccatgg tcatttttca gtcttaccat
     1261 aggggcataa actcaatttc cccatcaatg cggtgaacct atgcagtatt gtacaccatc
     1321 cagaaatcct cctcatccct ttccaagaga tggcaaacaa gtgacggact gaaccgcatc
     1381 tttggccaaa ttacctattc acaacgatgt cggtaaaact cacaattaaa acaggaggga
     1441 caggaagtgg aggacaacgg aagaggtttt caacaaagat acctagacta gttaatgttc
     1501 tgcacccctt gatttccgat cgtatcgaat aaagaggagg ggatgatcat gaaccgtaaa
     1561 ctatgcgcca ttaacgtaat agggtgtgtg ttattaggaa gtacattagc cctgggaaat
     1621 cccagcatgc tgccagacca tccagggtat cccatgggag acgcgaagtc tcccgtgacg
     1681 gggcaaccgg tagccaatga ccctggaaaa tctccgccca gccaggagga atcactccag
     1741 caagcggcgg gatttcatga tgcgcatgcc atgaatcctg gaaaggaaga acgaccgaat
     1801 attgtgtcgc ctggagcatt aaagtccatg gactcaaaga gtaagtccac gaaataagtg
     1861 ataattccac aatgtgttcc cgtacgatcg ggaacaccaa ggggtctaaa aaggaggggg
     1921 cttgaagctc cctcccttta ttcagcagga ttaccttcta tgtcactcaa acaaatattc
     1981 tagagagcca ccatccctcc ccctcgagtt agaacaacgt ataaatgaag ggggcaaccg
     2041 ctgaaccttc actcaggatt ataagaccgc cgagcaataa gagcatgatg atgatggggg
     2101 cgagccagaa cttttttcgt tcccgcataa agtcccacaa ttccccaaga aaacctgtca
     2161 tctgttgttc ctccttagaa gggtttcagc acatggtctg cggctctggc ctttttgacc
     2221 acgcgatagc tgttggcctt ggggtcatag ccaagttgca atggtcgttt acctaataat
     2281 cccatgataa acgccatggg agtaaacatt ccaaaaaaca gtaggcttaa gattatccgg
     2341 gaattgatcc agcctaattt ttcgccgatc ttcatccaga cgcgatgcaa gggcccaagc
     2401 ccttttggaa aaaccattcc cattgtccca aagaccgcgg cgataacact cgcccatatt
     2461 cgaaggcttt ctccatgaat gaccagaggc catacagcta cgattagaaa gactccgccc
     2521 attaacaggc caaacgagcg aagatccttg gcagttggtt gatgaatcgt tgatgtcata
     2581 tcgttatgct ttcacgctta atccaattca aattctttca tccacgtttc atccttttgc
     2641 accggctttt ggtgatcttt caaaacgaga caattttcca gcacaaggac atccatctcc
     2701 gtccgcatga aacaccggta ggcatcttca ggcgtacaga caatcggttc acctcgaaca
     2761 ttaaacgagg tattgatcaa caccgcacac ccggtttgct tttcaaactc ttgcaacaat
     2821 tgatagtagc gaggattagt atcaggtgac accgtctgaa ccctggcgga gtaatccaca
     2881 tgggtcactg ctggaatatc cgatttggga acattgagta gatcaattcc aaaaagtttt
     2941 tgttgatccg gttgtaccgc tcgacgtcgc ttctcggcaa ccggggccac cagtaacatg
     3001 tagggactat cggcatccaa ctcaaaatac tcggacactc gttctctcaa aaccgatggc
     3061 gcaaagggcc gaaaggattc ccgatatttt atttttaaat tcatgaccga ttgcattttc
     3121 tcgctacgag ggtcgcccaa gatacttcgt cctcctagag cccgaggacc gaattccatg
     3181 tgtccctgta accaaccaat gacctttccc tcagccaaat actctgctac tcgacgatac
     3241 agatccgaat cctccaggcg ctcatacctg gcttcaagct gttggagcct ggattcaatc
     3301 tcctcgttgg aaaagcgtgg ccctaaataa ctgccccgca tggaatcact aggaccctgg
     3361 catacccgag gctgattcag ataaatgtaa tgcacattta aggccgcccc caacgcgctc
     3421 cccgcatcgc ctgcggccgg ctgaatccac agcccgctga atgggccttc ccgcagcact
     3481 cttccgtttc cgacacaatt caaggccacc cctccggaaa ggcaaagatt ggaaagcccc
     3541 gtttctcgat gaaggctgtt ggccagacgg agcatgacca tctcggtgac ttcttgcaca
     3601 gaccgggcca gatccatttc ccgctgggtg agagtacttt ccgcctgacg cggaggacca
     3661 ccaaatatgg cgtcgaactt gttgttggtc atccgtaatc cggtacagta atcaaaatag
     3721 tccatattga gccggaaggt gccatccggc ttcacatcca ccacattgtc caaaatcgtt
     3781 ttcacatatt ttggctcacc atagggagcg agccccatca ctttatattc cccggaattg
     3841 accttaaacc cggtatagta ggtaaaggcg gaatacagca gcccgatgga atgaggaaag
     3901 ggaatttccc aaagaggtgt taattggttt ccccgcccga tccaggctga agtggtcgcc
     3961 cattcgccca ccccgtccat acataggacg accgcttcct cataaggaga agggaaaaag
     4021 gccgccgccg catgtgattc atggtgctcc ccaaaaagca aaggcggcag gttctctttt
     4081 ttttcgagac tccccacctg acgaaaagcc ttttctagta agtttcgcag aaatagcttt
     4141 tctttgatcc acacaggaat ggcggtcaaa aacgatccaa gtcccttcgg cgcataagac
     4201 aaataagttt ccaataaacg ttcaaatttg acaaaaggtt tgtcatagaa caccaggtaa
     4261 tcaacgtcgc gcatgccaac cccgccttgc cgcagacaat actcgaccgc atggctcggg
     4321 aacccggcat catgcttttt tcgcgtgaac cgttcttctt gagcagccgc cacaatcttg
     4381 ccattctgga tgagacaagc cgcgctatcg tggtaatagg cggaaacgcc cagaattgaa
     4441 gtcaatttgg cggttggctt tgtcatattt tccttagaat taggctttcc ttaagtatag
     4501 agactggggg taccaggcag aaaaccctcg aatcgtactt ttcggagaaa atcaaatcga
     4561 cttaaaagcc cctctttcct ttttcccata ctatcgacgg gaaaggtcaa actcaacttc
     4621 atcgaaatag ttcatcaaaa aacaatttca tgtgctccca tgaccgtcta tcagcttgct
     4681 cctgatattg cacaccgttc atcccatatt gattggccga ggggttggtg aatccatgtt
     4741 tggcccctcc aaaaatcacc atgtgccaat ctaaactagc ctcgtctaaa gcatgtttaa
     4801 atttcgtaat gtggtcttgg ctaaggaacg gatcggcctc tccatgggca atgagaattt
     4861 tgactgcact cttaatagca gaagatgcgg acggaagagg gagcgaccca tggaaactta
     4921 cgactccttt gaccggtgct ccatcataca ccatttgcat aaccgtcgct cctccaaaac
     4981 aatatccaat agctgctaaa cgggtttgat ctaccagggg gttagtctga agaagccgta
     5041 atccctcttt cgccctggcc tgccacatct ttacattggc tgtggtttgt tgcatccatt
     5101 gcctggcctc atcgggatga gtggtcactt ttccctttcc atacatatcc accgccaccg
     5161 ccacataacc cagggccgcc aattgttccg cacgagttcg ggcatattca tttagccccc
     5221 accattcgtg aacaaccaaa atccctggac gtttaccttt tatctcatca tcccaggcca
     5281 cgactccttc caaaacaact tcaccatggt tataggggat agttttgatc tgcaccctgg
     5341 cttccacttg cgaagaccac gcacacataa tcatgaggag acatagacct gatcgatgga
     5401 aataattcat gatttttgct tctctaatag ttcggtaata attcggacgg gaatgcaggc
     5461 tatgacatga attcgattta gtactatttc tggcagtcat taacctgaat cgctcccaaa
     5521 agggccagat actaaatttt acgataaatt cagcatttgg caatcttttt tgaattcatg
     5581 gtttttgggt ttcccacgaa tccgccagtt gtgctctcca accgagagaa agaagcacaa
     5641 tatccttttt tgaacggtag aactcaaata acacattggc acttaaatcg ggggcaccga
     5701 aaatgatttg ttgatactgt tccttagtca tcatcaccag acgcgtagat tgttgaacgt
     5761 ccctttttag ttcttccagc gccaaggaat ctttaagacc cttccctacg attcgtgaac
     5821 gaccgtggag ataatcccaa taaaattcat cttcatcccc ccgccaatcc catgactgaa
     5881 ataccaatat gggatcagtt tgtttattca gtcgccgttg attttcgtca aatgccagcc
     5941 tgggagagcc ttccgcttga atcccaggaa taataaatcc ggtcaccgtc accataaggg
     6001 ccacgcccaa cactcccata cccacaagac ccattcgcag ataattcctg acggccaggc
     6061 caatcattat caggcctaac atgcctaacc caacacgaag agctaaagga atgatgtcca
     6121 acggaacatg ccattttttt tgcaacagaa taggtcccag aaataccgaa accacaaaaa
     6181 tgccccctaa gcatgctaac aagcccctca acaaaccctt tccttctgat actggtgctt
     6241 catgcagtag tcgatggtac acaaacccga ccaacaaacc gaatgccggt aggaggggta
     6301 taaggtaccg ttcatgcttt tcgggcccca aactcatcgc cacaaagtac accacgaccc
     6361 agagaaagac ccacaattct ttcggcgact caagccaggg acgatgcttc catatgagaa
     6421 aaaggccaaa gggaagtaag gccgaccagg ggaaaaagtc gaaccagatt actagaccat
     6481 accaaaaaat gggacgtttg ccccaatagg cctcccacgc agaatattgt tgatcgcccg
     6541 atacgctgat catgcgtttg agattttctt caaagaaaaa atgctgcctg aattcggggc
     6601 caagtaacag gtagtaggca cccagtaaca agccactcaa tagggctcca acccaaaaat
     6661 accattcccg aaatactcgc ccatcacggc gcatccataa gaatattccc atcaccagaa
     6721 ccggcaaggc catggcatgt aacgttttaa ccatcgagcc caacgccaac gccacaaagc
     6781 cgattagata ccaccactgt gaagattttt gaaaatacgc gcgggcccag gcatacatcc
     6841 ccaacgtcat acaaaaagtt aacacaggat cgattatggc caatggacca aaccataagc
     6901 ccgcataaca ggtcgcacat accaacgcac tccaaaatcc tgccatttct gaaaacaaca
     6961 ggcggcctaa gtaataggtc gttatcatcg ttcccaaact ggatagaatc gacggcaacc
     7021 gtaaggcggc ctcactccat cccaaaccat gaatgaatcc cgcctgaagc caaaagaaaa
     7081 gaggcggttt actaaaatac ggctctcccc gcaaccaaag atgcacataa tcctcggccg
     7141 ccatcatggt gtgggtgata atggcgtaca aaccctcggt ttccccgaac agagaaatag
     7201 aggcattgga gctaaacgcc aaaaaaccaa tgactagaag aagcgaaata tgaatagcgg
     7261 ggcgttcaag gaacgcaatc caggaggtat tcattagaaa agagaagata tccaactgcc
     7321 catgatgatc attaccgaca aaaaacgttg gcacacccta caacaaaaac cctggacttg
     7381 ttaactcacg cgactatccg accaattcat ttattagaat cccggaaatg ggtactaacc
     7441 tggatatggt gaatgggcaa caatattttt atgcttgccc taattgaaga tatgccatgc
     7501 attagcagga aggaaaaaaa tacaacagaa gaatcgatgt ttcctggtat gtgattggct
     7561 tcaatattac gtaaacacgt aatgccctac gagtggggag tggggatact accatcatca
     7621 aggcaactgg ggaaatctga atggtgcgcc cggagggact tgaaccccca accctcggct
     7681 ccgaaggccg atgctctatc caattgagct acggacgcat gaagaattga ctttatacat
     7741 cccaccaagg tccccgtcaa ccaatctctt aattagtcag gcttcccctg gctgttctcg
     7801 cgtcttcata ttccctataa aggtaggtga tccgctcgta gctttcacct cttgggccca
     7861 cccctctggg gaatagtcac ggaaactcct attgggttga aatgggcttg agcaagattc
     7921 tttttcctat ttctgaacgt caggaatcaa atgatacgta ccgaacagtt ggcctctgat
     7981 accatcatgt ttcatgtgca aggaccgttt aataaacaag ttgccaaaaa attgggattg
     8041 ttggtcttcc gatcctacca cttagggtgt aagaccttcc tttgcaatct gagcctggcc
     8101 aaggcatttg ataaggatgc ttattcccaa ctgaccttga ttggagaagg attacgggat
     8161 aaaggaagaa attggcgagt aatttccccg ccctcttcca acggaaacca gcttatcctc
     8221 cgaacaagtc ttcagtttct tgttcccgca aacttgaatt aattactctt ctctaagaaa
     8281 gatcctatcg accatccttc tttgataaaa aatccttgct cgactccact ctttcccaaa
     8341 ttctcttaga attcacctaa tcacccaaaa attaagccat cccgcatttt gtaatccctg
     8401 tagaccgacg ccccaagaat cgctcatatt tttggaaatg aagctccgtt cacaaattgg
     8461 gagtatgggg gaaagatgaa gttgcccaaa aaaggaccgg ggcaccattc cgattgatcc
     8521 cgcaggaaat ttctctgcga ctattcccct atgaaaatcc cctcagcgag ctgtgggtat
     8581 cctgcgcagt tatgatgaaa taattaatac gattcaggag tagggagatc gacttcggat
     8641 tgggtggctt ctgggacaag tttttctaag cggcgatgat agtcacgaag ccgttgttgt
     8701 tgaggtggag tgaatgtaat agtttcctga ttgtgcaatt ccaccaactg ttcaacgcat
     8761 tgcaacagtg gagggacggc gtcaaatact ttccccgcct caaaggcttc taaggattcg
     8821 acgaataacg gagggagagt tttgtacgtg actcccactc ggtcacgaaa tcgatagacc
     8881 gtggtttcat aggcatcgac ggcctccggg gtcggacgaa ttccaccaga caatgccagc
     8941 atggacttgg ccggtacctt cgcggcaaaa gccgagagac tggcaatcag ttctcctaat
     9001 gtgtcaacca cctgcccagg ttccagtttt gcctcttttt tcatatgttg aaatctactc
     9061 caatcgcttg cctgcttgtc aagacgacca ttcgtcttca ttgcccttca aggcattcag
     9121 tacggtcacc atatcttccg gcaactccgc tgaaaatgtc accattttat tagaaacagg
     9181 atgtatgaag ccaagaactt tggcatgcaa catcacacgg ggaatgccaa tccctccaac
     9241 aaccatgact tttttccctc cataggtttg gtcgcccaag atcggacatc cgatagcctt
     9301 gaggtgaact cttagctgat gcgtgcgtcc tgtgccaggg aataactcta catgtgccgc
     9361 cgctgcccca aagcgttctt tcacccggta actggtcagc gacgccttgg gattggttgt
     9421 gcgtgcggaa aaggttttcc gatctttggt atctcgtccg atcgacaact caatccggcc
     9481 ttcccgtgtc tttgggcctt cccacacaaa cgcctcatag acgcgaatga tggaatgagc
     9541 tttgaattgt cccgccaata accggtgagc ctgatcattt ttcgccacga ctaagattcc
     9601 tgtcgtttcc ttgtcaagtc gatgcaccaa ccccggacgt tcctttcctc caatagttga
     9661 aagatgccct tccccgcttt gcatgtgatg caggagcgca ttaactaagg tcccggtcca
     9721 atgacccgga gccggatgaa ccacaaggcc tgccggtttg ttgagaacca ataggcattc
     9781 atcttcatag aggatgacaa ggggaattgg ttcaggattg attgccacgg gctctggacg
     9841 cggtacgacc agcacaacgc aatcaccagg cctgaccttg tggctgggtt tcacaacctg
     9901 accaccgacc gtaatatggc cgtcttcaat taatcgttgg agggctgttc gggaaaaata
     9961 gggttcgcgg ttggcaagaa aatgatcaag gcgtttgggc gattcgcctg cggtgacgat
    10021 gaactccgtc cgcgtagcga catgcggcat ggcagttaaa aaaacggggc ggtcgattac
    10081 tttatataag acgggaaccg ccctgttggc ggtggcgacc ggcaattttt tgccggaggc
    10141 gagccttttc caaactgacg cgagcttcct caacttctga tggtaggcca ccacgctcca
    10201 gacgttcttc cgcctttcga acggcatcct cggcccgttc tacattaatg tcttcagcct
    10261 tttcggcaat ttcggccaga attgttacct tagtcggcgt cacttcagca aacccccaga
    10321 gcacagccat gtattccgtc tgttcaccaa tccggtattg aagttcgcca attcgcaagg
    10381 tagtcagaaa atgacaatgg ccaggcagga caccgaaatc tccctcactc ccaggagctg
    10441 acacgtagtc gacttcctta ctgagcaaac ggtgatcggg ggtgaccacc tctaaaagaa
    10501 ttttctctga tgacgtcccc acaccagcac ctgccattat cgtttgtatc cgagtttttc
    10561 agctttttcg atggcttctt cgattggccc aaccatatag aaggcctgct ctggaaggtc
    10621 atcgtattta ccatccacga tttctttgaa gcttcgaacg gtatcaacga gtttcacata
    10681 tttcccaggt gatccagtaa aggcttctgc cacgtgaaag ggctgagaga gaaatcgttg
    10741 gagtttcctt gccctggcta ccgtttgctt gtcgtcctcc gataattcgt ccatacctaa
    10801 gatggcgatg atatcttgaa gatctttata ccgttgcaaa gtgccttgta cccgttgcac
    10861 aacctggtaa tgctcttcac ccaaaatatg gggatctaaa attcttgagg tggaatccaa
    10921 tggatcgacc gcaggataga tgcccaactc ggccaattgg cgtgacaaca cagtggtggc
    10981 atccaaatgg gcaaacgccg ttgccggggc tgggtcggtc aagtcatcag ccggcacata
    11041 aatcgcttga accgaagtga tggatccctt tttggtcgac gtgatccgtt cttgtaaagt
    11101 ccccatctcc gtacccagcg tcggttgata acccacggct gatggcattc gaccgagcaa
    11161 ggcagatacc tcggatccgg cttgagtaaa tcggaagatg ttg
//
LOCUS       DATIAL010000051         7229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_439729, whole genome shotgun sequence.
ACCESSION   DATIAL010000051 DATIAL010000000
VERSION     DATIAL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439729"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VLA60_01715"
     CDS             complement(<1..937)
                     /locus_tag="VLA60_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HSF08096.1"
                     /translation="MATYEKILLKNMEQPGYTGSLADYEGTGGYRALRTVLKDMQPDQ
                     VIEVVKKSGLRGRGGAGFPTGVKWGFIPKNHPGPKYLCCNADESEPGTFKDRQLMERD
                     PHQMLEGMAITCFAIGAQTAYIYIRGEFRLGAKILEQAIREAYAAGYLGDNILGSGLR
                     INIYVHLGAGAYICGEETALLESLEGKRGMPRFKPPFPATHGLYQNPTVVNNVETMAN
                     IPHIINRGGEWFASIGSPPKSCGTRVFCLSGHVKRPGNYETPMGITFRELIYDLGGGM
                     RSDKPLKAFIPGGASAPFLTPDHLDVKMDFESVALA"
     gene            complement(1019..1543)
                     /locus_tag="VLA60_01720"
     CDS             complement(1019..1543)
                     /locus_tag="VLA60_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSF08097.1"
                     /translation="MLFERFKNEVDDILSRYPVKRSALLPLLNLAQREEGFVSESAMQ
                     EIAKILDLTPAQVFETVTFYTMFNLKPIGKFHLQVCKSLMCALVGSDDVVVWIQKKLG
                     IAQGQTTADNLFTLSVVECLGSCGTGPMMQVNDDYYEQLSEEKVGRVLDDLKRDGTCS
                     LKTGPFMYPQPLAK"
     gene            complement(1633..3384)
                     /gene="nuoD"
                     /locus_tag="VLA60_01725"
     CDS             complement(1633..3384)
                     /gene="nuoD"
                     /locus_tag="VLA60_01725"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSF08098.1"
                     /translation="MHAFLESLVNRFPQAVLTAQQDPVRLDLWAQVQADHWLEIAQFL
                     HDDPAMALDHITDICSADYPDALERFEVIYHFLSLPHGIRIRIKARVTEDHPEIASIS
                     GIWRGANFLEREVYDLMGIRFTGHPDLRRILMPDDYDEGYPLRKDFPAEGKGWRSRFD
                     FLPRLDEPIGEWSESEVSDDQRHQFMADDHPGDTKRTTEMLLNMGPQHPSTHGVLRVV
                     LELDGERVSKATPDLGYLHRGVEKLAEGLTYMQMVPHTDRLDYVCAMTNNYAYVRAVE
                     KLIDMTVPERAEYVRTIVAEMQRIIGHLFWLGTQALDIGAMTVFFWTFREREILLDMF
                     EKLCGARLTLNYYRIGGVDADLTPEIITTLRGFLKTFPEHIREYDTLLQKNRIWVART
                     KGVALLSAEDAINFGCTGPVLRGSGVSYDIRKLEPYGVYDRVDWEVPLGKDGDTYDRY
                     WIRMEEMWQSARIIEQCLNQMPEGPIMAVDAQAIPPPKPEVMRDMQSLIHHFIIFTQG
                     FKPPKGETYCATEVPKGELGFFIISDGSSRPYRLKIRSPSFIHMGAFDHMARGYLISD
                     IVTIFGTYDIVMGECDR"
     gene            complement(3444..3917)
                     /locus_tag="VLA60_01730"
     CDS             complement(3444..3917)
                     /locus_tag="VLA60_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSF08099.1"
                     /translation="MLERQLEPNIITTNLDYCIAWCRKSALWPMTFGLACCAIEMIAS
                     VSSKYDIDRFGAGVFRASPRQSDLMIVAGTVCRKMAPVIRRIYDQMAEPRYVISMGSC
                     ATSGNHYNSYSVVQGVDQIVPVDVYIAGCPPRPEALMDGLIKLQEKIQKEKYFVK"
     gene            complement(4041..4412)
                     /gene="ndhC"
                     /locus_tag="VLA60_01735"
     CDS             complement(4041..4412)
                     /gene="ndhC"
                     /locus_tag="VLA60_01735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSF08100.1"
                     /translation="MGSEPVPFNYLPIVIFMVIGALFGVGSLVVGWFVRPSRPYRAKL
                     LPYESGAPLFMDARVQFPMRYYIIAMLFVIFDIEVVFLFPWAVVFKKLGLLGLVEMGV
                     FLAILIVGFWYAWKKGALDWD"
     gene            complement(4486..5634)
                     /locus_tag="VLA60_01740"
     CDS             complement(4486..5634)
                     /locus_tag="VLA60_01740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSF08101.1"
                     /translation="MSEIGLHGPMSFARFMELALYDVPHGYYMTKTVEPDLSSSDRIG
                     WGGDYFTAPELSPILAKTLVRQILAIDDQLGHPPQFTFMEIGAGNGTFAFDFIQECRT
                     IASDFFHRLSYQIIERSSHLQSRQEARLSTLGGGWGESHLSWKSSIEEVDSDSITGVV
                     FSNELVDALPVHRVRMADQRLHEICVAYKSGRLVECLDHRLPPELIEYIETHKVALSE
                     GQTSELHLAAELWMKHVARLLHRGIVVTVDYGHTHRDYYRSDRKDGTFLCYHRHAIST
                     DPYVRVGEQDMTAHVNFSALASVGMTNGLDLVGLTTMANWLIGLGVEELVRDQDQESD
                     DVRALAHLLRPHGMGTTFKVLVQRKGMEAFAIQGLRYPAFFDDVVFSN"
     gene            complement(5925..>7229)
                     /locus_tag="VLA60_01745"
     CDS             complement(5925..>7229)
                     /locus_tag="VLA60_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HSF08102.1"
                     /translation="RGVKHHVLNAKFHEKEAEIIAQAGQKGAVTIATNMAGRGTDILL
                     GGNAEALFKQQVIYRGEDLTEEEQQKAYATIKAACDQNKKEVLAAGGLHIVGTERHES
                     RRIDNQLRGRAGRQGDPGSSRFFLSLEDDLLRIFASERVSNLMLKLGMEEGVPIEHRM
                     VTKSIENAQKKVEGHNFEIRKHLLEYDDVMSKQREVIYKHRQNVLRATDVTEDVFGMI
                     EEVGERFVDIYCPEEQYAEEWDIAGLVETVQGQFGVDVIQAEEIKDVGRDALKEEIPE
                     KLKQAYRNKVQALIHEHGATEDLVHYVERTILLQMIDHHWKDHLWGMDQLKDGIGLRG
                     YGQKDPLAEYKREGYDMFAGMMERIKNDTLERLFKFQLVRGERPEPEREAPRPAQLNL
                     NRGGESAATPQQRTVQRAEEKVGRNDPCPCGSGKKYKKCHGT"
BASE COUNT         1878 a         2012 c         1586 g         1753 t
ORIGIN      
        1 ccgctaaagc cactgactca aaatccattt tcacatctaa atgatcaggc gtcagaaatg
       61 gagccgatgc tccccctggg ataaaggcct taaggggctt atccgatcgc atacccccgc
      121 ccaaatcata aatcaattca cggaaggtga tgcccatggg cgtctcataa ttaccaggcc
      181 gcttcacatg tccgcttaaa caaaacaccc gcgttccgca acttttcggc ggagagccaa
      241 tcgaggcaaa ccactctccc cctcgattga taatatgagg aatattcgcc atcgtctcga
      301 cgttattcac cacagtgggg ttttgataca atccatgcgt cgcaggaaac ggcggcttaa
      361 agcgaggcat gccccgtttg ccctctaacg attcgagcag tgccgtttct tccccacaaa
      421 tataggctcc ggcaccaagg tgaacataga tattgatcct caacccgctc ccgagaatgt
      481 tgtcgcccag atacccggcc gcataggcct cccgaatagc ctgctccaga atcttcgcgc
      541 cgagacgaaa ctctccccga atgtagatgt aagccgtttg tgcgccaatg gcaaaacagg
      601 taatggccat cccttccagc atttgatgcg gatcacgttc cataagttgc cggtctttaa
      661 atgtccccgg ctcgctttca tccgcgttac agcacagata tttaggacca ggatgattct
      721 tcggaatgaa cccccatttc acgccggtgg ggaacccggc tcccccacga ccgcgaagac
      781 ctgacttttt taccacctca atcacctgat cgggctgcat atccttgaga actgtccgga
      841 gagctcgata cccacccgtc ccttcataat cggccaacga accggtgtat cccggttgtt
      901 ccatattctt caataagatt ttttcgtaag tggccataca gacactgttt cgtgaacggg
      961 cagttattga taacccatga attgcttatc catgactatt ccctgcaaaa gacttttgtc
     1021 atttagcaag gggctgagga tacatgaagg gtcctgtttt aagcgaacag gttccgtctc
     1081 gtttcaaatc gtccaggacg cgtccgacct tttcctcgga taattgctca taatagtcat
     1141 cattcacttg catcatggga cctgtaccac aggacccaag gcactccacc acgctgaggg
     1201 taaagaggtt atctgccgtt gtctgaccct gtgcaatgcc cagttttttc tggatccata
     1261 ccaccacatc atcagatccg actaacgcac acataagcga tttgcacact tgaagatgaa
     1321 acttcccgat cggcttgaga ttgaacatgg tataaaacgt gacggtctca aacacttgtg
     1381 caggcgtcaa atccaaaatt ttggcaatct cctgcatggc cgattcactc acaaatccct
     1441 cttcccgctg cgccaagttc aatagcggca acaacgccga tcgctttacg gggtagcggg
     1501 aaagaatatc atccacttcg tttttgaatc gctcaaatag catagaacac tcgtgagttg
     1561 agacgagccg tcaacgccca atggccatgg tgtccgtacg ctgctcttcc ctccatcaaa
     1621 ccattctcca tgttatcgat cgcattcccc catcacaata tcgtacgttc cgaaaatcgt
     1681 gacgatatcc gaaatcaaat atccacgcgc catatgatca aaggcgccca tatgaataaa
     1741 ggaaggagag cggattttca accgataggg acgcgaactc ccgtcactaa tgataaaaaa
     1801 gcccaattca cctttaggca cctccgtcgc acaatacgtt tcgcccttcg gtggcttaaa
     1861 cccttgcgta aagataatga aatgatgaat caggctttgc atatccctca ttacctctgg
     1921 cttgggaggt ggaatggctt gagcatcgac ggccatgatc ggcccctcag gcatttgatt
     1981 gaggcattgc tcaatgatgc gggcgctttg ccacatttct tccattctga tccaataccg
     2041 gtcgtaggta tccccgtctt ttccaagtgg cacttcccaa tccacacgat catacacgcc
     2101 atacggctcc aacttccgaa tatcatagga cactcccgac ccccgcagaa ccggccctgt
     2161 gcatccgaaa ttgatggcat cctccgcaga aaggagtgcc acgcctttgg tgcgggcaac
     2221 ccaaattcta tttttttgca agagcgtgtc atattcccga atgtgttcgg gaaacgtttt
     2281 caaaaaaccc cggagagtcg taataatttc cggcgtaaga tctgcatcca cgcctccaat
     2341 ccgataataa ttcaatgtca gacgagcgcc acacaatttc tcaaacatat ccaacaagat
     2401 ttcacgctcc cggaaggtcc agaagaacac ggtcatcgcc ccaatatcca gggcttgtgt
     2461 tcccagccag aataaatgac caatgatacg ttgcatttcg gcaacgatgg tccgcacata
     2521 ttccgcccgt tccggaacgg tcatatctat cagtttttct acggcccgca catacgcata
     2581 gttattggtc atggcacaga catagtccaa gcgatcggta tgcgggacca tttgcatata
     2641 cgtcaggcct tcagccagtt tttccactcc acggtgcaaa tatccaagat cgggcgtggc
     2701 cttgctgacc cgctcaccat ccaactccaa cacgactcga agcacaccat gcgtactggg
     2761 atgttgtgga cccatattga gaagcatttc cgttgtgcgt ttggtgtcac ctggatgatc
     2821 atccgccatg aattgatggc gttgatcatc ggacacctcg gattccgacc attcgccgat
     2881 cggttcatcc aacctgggaa gaaaatcaaa ccggctccgc caccctttcc cttcggccgg
     2941 aaaatctttt cgtaacggat acccttcatc ataatcatca ggcatcaaaa ttcgacgaag
     3001 gtcgggatgc ccggtaaagc ggatgcccat caagtcgtag acttcacgtt cgaggaaatt
     3061 ggcccctctc cagatcccac tgattgaggc gatctccgga tgatcctctg tcacgcgagc
     3121 tttaatccgg atccggattc catggggaag ggagagaaag tgataaatga cttcaaatcg
     3181 ttcgagggca tcgggataat ccgccgaaca gatatcggta atatgatcca acgccatggc
     3241 gggatcgtca tggagaaatt gcgcaatttc cagccaatgg tcggcttgca cttgtgccca
     3301 caaatccaac cgcacgggat cctgctgcgc cgtcagcaca gcctgaggaa agcggttgac
     3361 cagagattcg agaaaagcat gcataaatcg aatttgttgc tgaatcgtgg aatatcgaaa
     3421 gaccgtctac acaacttcca gagctatttc acgaagtatt tttctttctg aattttttct
     3481 tggagtttaa tgagaccatc catgagtgcc tcaggtcttg ggggacaacc tgcgatatag
     3541 acatccacag ggacaatttg atccacaccc tgaaccacac tgtaactatt gtaatgattt
     3601 ccggatgtcg cgcaggatcc catggagatc acatatctgg gctccgccat ttgatcataa
     3661 attcgacgga tcaccggtgc cattttccga cagaccgtcc cagccacaat catcaaatcg
     3721 gactgacgtg gtgacgcgcg aaacaccccc gccccaaaac gatcgatgtc atacttggaa
     3781 gacaccgagg cgatcatttc aatcgcacag caggctaacc cgaaggtcat cggccaaagg
     3841 gctgatttcc ggcaccaggc aatacaatag tccaggttgg tcgtaattat attcggttca
     3901 agctgtcgtt caagcatccc cataagatta ccgtgggtac ctcaatgttc tgtatgaagc
     3961 tcgttgccta cccccaaaag atgggggtgc cattgtactg ggtggactcc ggttttttcc
     4021 aggagaaaag aactattttc ttaatcccaa tctaacgcac cttttttcca ggcataccag
     4081 aaccccacaa tgagaatggc cagaaacact cccatttcaa caaggccaag aaggccaagc
     4141 ttcttaaaca ccaccgccca aggaaataag aacacgacct caatatcaaa tatgacaaag
     4201 agcatggcaa tgatgtagta gcgcatcgga aactgaaccc tggcgtccat aaacaacgga
     4261 gcgccacttt cgtaaggaag cagcttcgcc cgatagggcc ggcttggacg gacaaaccac
     4321 cccacaacca aggacccgac tccaaacagt gccccaataa ccatgaagat gacgatgggg
     4381 agataattaa aaggaaccgg ttcactaccc atagatcact cgcattccaa aataagtcat
     4441 actcacgacc ctaactccgc ttactatctt tcttctccgc cttcttcaat tcgagaacac
     4501 cacgtcatca aaaaaagcag gataacgcaa cccttgaatg gcgaacgcct ccatcccttt
     4561 tcgctgaaca agaaccttaa acgttgtccc cataccatgc ggacgtaata aatgcgctaa
     4621 cgcccttaca tcatcagact cctgatcctg gtctcgcacc aattcttcga ctcctaaccc
     4681 gatcagccaa ttggccatag tcgtcaatcc gacgaggtcc aacccattgg tcatcccaac
     4741 gcttgccaag gcagaaaagt tgacgtgagc tgtcatatcc tgctctccaa cccttacata
     4801 cggatcggtc gaaatggcat ggcgatgata gcataagaag gtcccgtcct ttcggtcgga
     4861 ccgataataa tcacgatgcg tatgaccata gtcgacggtc acgacgatac cgcggtggag
     4921 aaggcgagcc acatgcttca tccaaagctc agcggcaaga tggagttcgg aggtttgccc
     4981 ttcactcaag gctaccttat gcgtctcgat gtattcgatc aattccggag gcagacgatg
     5041 atccagacat tcaaccaaac gcccgctttt gtaggccaca catatttcat gaaggcgttg
     5101 gtcagccatt cggacccggt ggacaggtaa cgcatcaact aattcgttgg aaaagacaac
     5161 tccggttatg gaatccgagt caacctcctc tatggaagat ttccaactca ggtgactttc
     5221 cccccagccc ccccctaagg tgctgagcct ggcctcctga cgcgattgga gatgggaact
     5281 ccgctcgatg atttgataag aaaggcggtg aaagaaatca gaagcgatcg ttcggcattc
     5341 ttgaatgaaa tcgaaggcaa aggttccgtt ccctgcacca atctccataa aagtaaactg
     5401 agggggatgg cctaactggt catcgatagc caaaatctgg cgaaccaatg ttttcgccaa
     5461 aatgggggag agttccggtg cagtaaaata atcaccaccc catccgattc ggtctgagga
     5521 ggagaggtca ggttcaaccg ttttggtcat ataatatccg tgtggaacat cgtagagggc
     5581 caattccatg aaacgcgcaa aactcatggg cccatgaagg ccaatttcag acaaaatctt
     5641 ctcgataagg tctggcttac tctgtatttt ctgtggttcg gatggcacga atggactatg
     5701 gtcactcctt caatatgcct attgagaatc ggttacgata acggcctacc ttcagccaag
     5761 tcaagcaatt tcgtactttt taaggcaata aaaaacaata gattcaatat gttatggacg
     5821 cgagcctggt caggagagaa atgaagagca gtggtaaaca gaaagggaga gccgtcacaa
     5881 caagaccggc atcagggagc agagcaagga gggcctttca ggtattaggt cccatggcat
     5941 ttcttgtatt ttttcccgct cccacatggg cagggatcgt tccgtccgac tttctcttcg
     6001 gcacgttgaa cggtgcgttg ttgaggagtg gcagcggatt cacctccacg gttcaaattt
     6061 aattgtgccg ggcggggcgc ttcccgctca ggctcaggcc gttccccccg aaccaattga
     6121 aatttaaaga gccgttctaa ggtatcattt ttaatccgtt ccatcatacc ggcgaacatg
     6181 tcatagcctt cacgcttata ttcggcgagg ggatcctttt gcccataccc gcgcaggccg
     6241 attccatcct tcaattggtc cattccccac aagtgatctt tccaatgatg gtcaatcatt
     6301 tgaagcagaa tcgtgcgctc aacataatgc actaaatcct cggtggcgcc atgctcatga
     6361 attaaggctt ggactttgtt ccgataggcc tgctttaatt tttctggaat ttcttccttg
     6421 agcgcgtctc gaccgacgtc tttgatctcc tccgcctgaa tcacatccac gccgaattgt
     6481 ccctgcaccg tttcgactaa tcccgcaata tcccattctt cggcatattg ctcctcagga
     6541 caataaatat cgacgaagcg ctcgcccact tcctcgatca ttccgaaaac gtcttctgtc
     6601 acatcggtgg cgcgtaacac attctgtcga tgtttataga taacctcccg ctgcttactc
     6661 atcacatcat catattcaag aaggtgtttg cgaatttcaa agttatggcc ttcgactttc
     6721 ttttgcgcgt tttcaatgga tttggtgacc atccggtgct caatgggcac cccctcctcc
     6781 atgccgagct tgagcatgag gtttgagacc cgttcagacg caaagatacg aagcaaatca
     6841 tcttccaagg acaggaaaaa ccgcgaggag ccaggatctc cctgccgacc agcccggcct
     6901 ctcaactgat tgtcgatccg acggctctca tgacgctcgg tcccgacgat atgtaaccct
     6961 cccgcagcca aaacttcctt tttattctga tcgcaagcgg ctttaatcgt ggcgtaggct
     7021 ttttgttgct cctcttctgt cagatcttct ccacgataaa taacctgttg tttgaagagc
     7081 gcttcagcat tgcctcccaa taaaatatcg gtgcctcggc cggccatgtt ggtcgcaatc
     7141 gtcaccgcac ccttttgccc ggcctgggca ataatttcgg cttctttttc atggaacttg
     7201 gcattcaaga catgatgctt cacaccccg
//
LOCUS       DATIAL010000052         4152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1192606, whole genome shotgun sequence.
ACCESSION   DATIAL010000052 DATIAL010000000
VERSION     DATIAL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192606"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            681..2327
                     /locus_tag="VLA60_01750"
     CDS             681..2327
                     /locus_tag="VLA60_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08103.1"
                     /translation="MKIWLMGFCVFLITGGVGAEAASPAKRCTGWEEGKGHCKSKGAS
                     WDKHGSEARTLCEAGNIIQVSDIVESAALDLLRRDYLLLPIGENPSELFPTHVVIAPQ
                     DLDDPGTIALLREAHQVGKTVAIVGANQVEARTFHRLLRPGQEVNCAPQPGQTQIPLY
                     GLQQSVTRVPGQNSTYCLGNLDRHRGNRKAERRWLKDRFAATPPQPREGDVSGTSDPT
                     TFLTDLATATHCSFKFPNNGAGTAEVDYYVYGMRNFSDTGCSSCSSVGADYYLVQEDS
                     TFEPTSGNWFSTVSANLSDASGELIDTNISGLLFTDPQTVTTVETSYTNDSGTTVSGS
                     VGLNSDGPNVTAGGSVTSGQSTTYSMPATTINNASDPKSLNVDWNFTPQTPIPNTEYD
                     YSPTWTWFVPQDAYPNGGTGNGQVTFTSYPNVGQLLSDGIFIDQTIWEGQCNIPYPFS
                     AWTVSPPQLTSFNPPTTQKDGGQFTITGEFLYPGSVTAVLIGGQSINLGTNVDFMDDT
                     TIQVVVGNLTLAAGTYSVQVNTQFNGQNRFSNTLELELTN"
     gene            complement(2894..3571)
                     /locus_tag="VLA60_01755"
     CDS             complement(2894..3571)
                     /locus_tag="VLA60_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HSF08104.1"
                     /translation="MPFFKTIRWVVWKDLISEWRTRETMSSMLFFALIVILVFSFSFS
                     MDQDAARQLIAGIIWVAFTFTGIIGLGKSFTSELQNDCLESLQMCPAPKGAIYLGKVA
                     ANFLFMLSVEILLFPLFVLFFNLDVIEAVGILLIIFFLATLGLSAVGTLFSALTVQIR
                     AREVMLPVLLLPLAVPVMIAAVEATRGALSGDPFSFYSQWLQLLIIFDIIFTVVSFWL
                     FEFILDS"
     gene            complement(3558..>4152)
                     /gene="ccmA"
                     /locus_tag="VLA60_01760"
     CDS             complement(3558..>4152)
                     /gene="ccmA"
                     /locus_tag="VLA60_01760"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HSF08105.1"
                     /translation="PGECFALFGPNGAGKTTLLRILATLQAPSHGQFTIFGQDGVAQK
                     DTVREIIMLIAHGSHLYDELSATENLQFALGLRGQTPSPPHIKRALDRTGIGAFADLK
                     IRQFSAGMKKRLDFAKTILAQPQLLLLDEPYNALDHDGVAITNQLIQETLDRDGTVFM
                     TTHDPDKATQIATRGGILKGGHLQLLSSEQLTTDAIF"
BASE COUNT         1029 a         1035 c         1135 g          953 t
ORIGIN      
        1 gtgcttgtgt gagccaaggg cacatgcaca tacatgatcc tgaccagttc accttgataa
       61 taatcaggcg gtgaggcgag caagccgata tacaagccta caaaaatgca aagagcggcc
      121 cctccgccaa accatccttt atagcgttgg atctgtcgga taatgcgatt gatcatgttg
      181 gacattgtaa aacggataac ccgacggagg gaagggctaa ctccttcaaa tactgttggt
      241 aagagttttg ggtcattgaa acccaaaagg aagttccagc aggtgttcca tgacaatgaa
      301 tcagatggcc tttcttcatc atttccggat attagcttca ccctatggta ggtaatgaac
      361 gcattctgcg gcctgcgtga agcacgggtc taagattggg ctgaattacg ccaatgcgtc
      421 caccaactca acgttgacta tggatgatga tatatccctg aagcctaaat ctgtatgacc
      481 atcgaatcat cggcatatct cttcaatcgg gccggaggca aatgaggaga tgcgagccgc
      541 cgtgccattg tgcgacataa aactttctat ttcctttttt taatttttta cgttcacact
      601 ttcaactaat aaccaaacgc tgggtaaagc cagggcgaag tgaggctcca gcaagagaga
      661 aggacactat gcgagacctt atgaaaatct ggttgatggg attttgtgtt tttctcatca
      721 ctggaggcgt tggggcagag gcggcttccc ccgcgaagcg ttgcacaggg tgggaagaag
      781 gaaaggggca ttgtaagagt aagggcgcct cctgggacaa acatgggtcg gaagctcgca
      841 ccttgtgtga ggcaggcaat atcatccaag tctctgacat tgttgagagc gcggcgcttg
      901 atctgctgcg ccgcgattat ctgctccttc cgatcgggga gaacccttcc gagctttttc
      961 cgacacatgt agtgatcgca ccacaagacc tcgacgaccc ggggactatt gccttgctaa
     1021 gagaggctca ccaggtcggc aagaccgtgg ccatcgtcgg tgcaaaccag gttgaagccc
     1081 gaacattcca ccgcctgctg cgcccagggc aggaggtgaa ttgcgcccca caaccggggc
     1141 aaacacagat tccgctctat ggattacaac agtccgtcac cagagtcccc ggacaaaact
     1201 ccacttattg tttggggaac ttggacaggc accgcggcaa tcgtaaggcg gaacggcgct
     1261 ggttgaaaga tcgctttgcg gccacgccac cgcaacctcg tgagggcgat gtcagcggga
     1321 cgagcgatcc gaccacgttc ttaacggacc ttgccaccgc gacgcactgc agcttcaaat
     1381 ttcccaacaa tggtgcgggg accgccgagg tggactacta cgtctatgga atgcgcaact
     1441 tctccgatac cggctgtagt tcctgcagtt ctgtcggcgc agactactat ctcgttcagg
     1501 aggactcaac gtttgagccc acgtcaggga actggttcag cactgtctct gccaatttgt
     1561 ccgacgcatc gggtgagttg atcgatacga acatttccgg tcttttattc acagaccctc
     1621 aaacggtgac gacagtcgag acctcctaca ccaacgactc gggcacgacg gtaagcgggt
     1681 cagttggatt gaattcggat ggccccaacg ttacagcggg tggctccgta acgtccggcc
     1741 aatcaacgac ctacagcatg cccgcaacga cgatcaacaa cgcctccgat ccgaagtcat
     1801 tgaacgttga ctggaatttt actccacaaa cgccaatccc gaacaccgaa tacgattact
     1861 cgccgacttg gacgtggttt gtaccccaag atgcttatcc caacggcggg acgggcaacg
     1921 gacaagtcac tttcacctca taccccaatg tcgggcagtt gctctcggat gggattttca
     1981 tcgatcagac gatttgggaa gggcagtgta atatccccta cccgttctca gcatggacgg
     2041 tctcgccgcc gcagctgacg agcttcaacc cgcctaccac ccagaaggat ggcggtcagt
     2101 tcacgatcac cggggagttt ctgtatcccg gcagtgtgac ggctgtgctc attggcggac
     2161 agtctatcaa cctcggaaca aatgtcgatt tcatggatga caccacgatc caggtcgtcg
     2221 taggcaattt gacattggca gcaggcacct actccgttca agtcaacacg cagtttaatg
     2281 gccagaatcg tttctccaac acactggagt tggaattgac gaattgacat cttctgtccc
     2341 tatggttggc cgcgccacat ggcggctgtg agccacgagg atcgggtgat gcggggctga
     2401 ataggaggat gtctttcttc tgaaaggata ttccctctct ccagaatccg tttgaatgct
     2461 ttgccggaaa aaggatttgg catgtaggat cacgggctgc ccagttaatg actaaagcgt
     2521 ttcgctgagc tcattttgac caatcggata cagcctgcct ccgcctccca tttggtcaaa
     2581 gcgaatagat agattggccg actttcccgt gttgtcttgc cctgttttga cctctgcaac
     2641 aatctttctg ccccgtcgaa ttggtccttt atctcagact gatgggagac ctcgtgatga
     2701 cccgcaccgg tttgcaaaga gaggttcact ggtgtcatcg atattgtcaa ggattgtgat
     2761 gtcggggcct ggaggcgcag ggattgattg gcggttccgg tgaactgctg agcgggaata
     2821 tttcggtcca aaatggtatc caggccggct gagagcggga gcaggattgt gctgatgaac
     2881 ggactgtgga tacttacgaa tctaatatga attcaaagag ccagaatgag acgacggtga
     2941 aaataatgtc aaagatgatc aagagttgga gccattgcga gtaaaaagaa aatgggtctc
     3001 cgcttaaggc tccgcgtgtg gcttctactg cggcgatcat gaccggaacg gccaggggca
     3061 gaaggaggac ggggagcatg acttcccggg ctcgaatctg cacggttaag gctgaaaaga
     3121 gtgtgccgac ggctgatagc cccaaggtgg ctagaaaaaa tatgatcaac aggattccta
     3181 cggcttcaat gacatccaga ttaaagaata agacgaacaa ggggaataag aggatttcga
     3241 ctgagagcat gaagaggaaa ttggccgcga cttttccgag ataaatcgcg cccttgggag
     3301 cggggcacat ttgcagggac tccagacaat cgttttgcaa ttccgaggta aaggatttac
     3361 ctaagccgat aatgcccgta aaggtaaagg cgacccagat gattcccgca atcagttggc
     3421 gtgccgcatc ctggtccatg gaaaagctga agctaaatac cagaatgaca atgagggcaa
     3481 agaacaacat cgacgacatg gtttcccgtg tccgccattc actgatcaga tccttccaga
     3541 cgacccagcg aattgtttta aaaaatggca tcggtggtta attgctcaga ggagagaagt
     3601 tgaaggtgtc cgcctttgag aatcccgccc cgtgtagcga tttgggtggc cttgtcaggg
     3661 tcatgggttg tcatgaagac ggtgccgtca cggtccagcg tttcctgaat gagctggttg
     3721 gtgatagcca ccccgtcatg atccagggcg ttgtagggct catcgagtag aaggagctgc
     3781 ggctgagcca gaatggtttt tgcgaaatcc agtcgctttt tcatgccggc cgaaaactga
     3841 cgtattttga ggtcggcaaa ggccccgata cctgttcgat ccagtgcccg tttgatgtga
     3901 ggtggagatg gggtttgtcc gcgcaggccc agggcaaatt ggagattttc ggtggcactg
     3961 agttcatcat ataaatggga tccgtgagcg atcagcatga tgatttctcg aacggtatcc
     4021 ttctgtgcca cgccatcctg gccaaaaatg gtaaactgac catgagaagg cgcttggagg
     4081 gtggctaaaa tcctgagcaa cgtcgttttt cctgccccgt tggggccaaa taaggcaaag
     4141 cactctcccg ga
//
LOCUS       DATIAL010000053         8217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_655203, whole genome shotgun sequence.
ACCESSION   DATIAL010000053 DATIAL010000000
VERSION     DATIAL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655203"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VLA60_01765"
     CDS             <1..401
                     /locus_tag="VLA60_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08106.1"
                     /translation="QMQALQAVQGIRLIEQEGEPLVRPYESMVDALYERLRALHGSAY
                     SWTPPPVSAVEKLSSTRMREYVRGWITEWDLRRLYPEAQLDIEILDVRQTYDEDQELE
                     PTMADDIGNEKVSESEESLMAVALQNQAPV"
     gene            769..2511
                     /locus_tag="VLA60_01770"
     CDS             769..2511
                     /locus_tag="VLA60_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08107.1"
                     /translation="MSVSELTPRKELPLSVLGLWRTCVNRILGAMELLHRHTILVLTV
                     LFGLCLIVIYLHISQLKTQMVTAMAFRGSEIYTRALNEMRIWYTVEVVQRLHNQGIEA
                     STGYRDHQGEIPLPATLSRDLGNRLVAGRKGERLELLSPYPFYPGKKSGGPIDGFQEE
                     AWEFFMQHPDTDEPFYRFEQVDGRESLRFVKADRMQKECVECHNSHPDSPKRDWQIGQ
                     VGGLVEVVYPVDSLETIMGMSWQGTYGLMGMLVVIWMGGFGLVVLKLGRVASELEEQV
                     RDRTGALQSANKHLELEIHDRLLAEDTLRKAKDNLEKRVQERTGKLAASNAELIREVA
                     ERKRAEEDIRKLNSQLVQRSAQLEASNKELEAFSYSVSHDLRAPLRGIDGFSQAVLED
                     YDEKLDESGRNYLRRVRTASQRMSKLIDAMLNLARLTRAEIHTQTFDLSAVARAVLED
                     FQKMDPDRQVECIVANNVFATADPQLLRAVLENLIGNAWKFTQQQTHPRIEFGYGQYK
                     GQAAYFVSDNGAGFDMTYAHKLFGAFQRLHAYTEFPGVGVGLATVHRIIQRHGGQIWA
                     EGVVGKGATFHFTL"
     gene            2532..2978
                     /locus_tag="VLA60_01775"
     CDS             2532..2978
                     /locus_tag="VLA60_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08108.1"
                     /translation="MMTNKVILLVEDHPDDEELTMRALKKNNIKNEVVVARDGVEALE
                     YLFGTGAHAGRDLSQMPQIILLDLKLPKIDGLEVLRRLRADERTKFLPVVVLTSSKEE
                     QDIVKSYQLGANSYVRKPVDFQEFSQAVQNLGLYWLILNESAPERV"
     gene            3355..3747
                     /locus_tag="VLA60_01780"
     CDS             3355..3747
                     /locus_tag="VLA60_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08109.1"
                     /translation="MRRMLTWDIPESVTSHEHAWNPKRKYMAESFGLETQRASRSSAA
                     GGICCAKCGSEVKVFVSPRRNPHSSKLGRAVSLKDHDLCRRCWRKLMHEQRKTGVVTL
                     PISFFVKEENLRPRLLIPKMASVPKQAS"
     gene            complement(3832..4392)
                     /locus_tag="VLA60_01785"
     CDS             complement(3832..4392)
                     /locus_tag="VLA60_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08110.1"
                     /translation="MKLLSGILSIRTCALFTGASIVLSGGLGLAEPGIDGFRELKFGM
                     TEQEVSALPACSNSKECLYELTDKNRYVELTYLPEEVSDTTEPTGKLAKITIDMGLYR
                     DEWYQQLQIILGASYHLTQDLAEATKQSFLAGQLDELNSGFEDGQVLLKVVRRPFGNL
                     ELKVVYQNAALAKAFIQERQATISSK"
     gene            complement(4878..5861)
                     /locus_tag="VLA60_01790"
     CDS             complement(4878..5861)
                     /locus_tag="VLA60_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08111.1"
                     /translation="MASFRYFLAGVALTSALMVSPALADWESKPFTWGPWALYLEMKD
                     AAGLGLRNVSYQGNQILGKADLPVIRVKYVREWPAWHPFSWFGLGRSSGRCGPFEDRI
                     SPQRLKKNPNCDGKKLCQAEHTIQGVKWLELGVYAKIGQYHLYQAWYLSEDGRLYPSV
                     QSWNLSCNTDHEHHAYWRLDFDIGGSDQDQVFVLDRNSVKDKGWGPGWEKYLVEEDDK
                     KPGNNSQDRVWFVRDYPTGQGVWVIPGPFDGESKKFSDRDVSIRKFYRDEDAGWPFGA
                     RGDLEFKSDESVQETNVVFWYMAHLPHKAAEGDRPMRYWMGPLLQVHQETP"
     gene            complement(5888..6694)
                     /locus_tag="VLA60_01795"
     CDS             complement(5888..6694)
                     /locus_tag="VLA60_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08112.1"
                     /translation="MHSKFTSHILVTAGWVLGSFFLGCSSVELTKIPKKPLPFHPPPL
                     IELPLELPPEGGPRRPVDAMSPTTLSGPGPEIIIVESDQPALRATAERDPDVKTQLGD
                     RFAYIDTEEVPADQCLVTLQADNTTHTRSTERGSASDASAYRLTYYSYTHNVAVHVCM
                     ENDRMSSVQQDPREGYQPEEGEEEITTAIELARVDQRIAQEVQHLHGHAILTSPEEYR
                     YFWVSDEAGFGDRVFWVTFSETPESLALYFARVDLTSQTVLDAGKEAGPQ"
     gene            7657..>8217
                     /locus_tag="VLA60_01800"
     CDS             7657..>8217
                     /locus_tag="VLA60_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08113.1"
                     /translation="MTSRLTHRTLLWFSLILLTIFGFFLIDVGLYFLIPPKWVQFAPD
                     YRLHPKKTPLGGLGKGVSRYYYQADPLLGFDITPLAKGTHFILDGVENEIFANELGCF
                     DRNSLEDFVASKKDYVYFAGDSFTWGFARYEDKFATVWENNTGMLTAKCGVGHTGTIH
                     QFGKFKKIIETIGRQPTVVFVGFFPND"
BASE COUNT         2065 a         1872 c         2183 g         2097 t
ORIGIN      
        1 ctcaaatgca agccttacag gcggtccagg gtatccggct catcgaacag gagggcgaac
       61 cattagttcg tccctatgaa tcaatggtcg atgcgctata tgagcggctt cgggcgcttc
      121 atggatcggc ctatagttgg accccgccac ccgtttcagc tgtagagaaa ttatccagta
      181 cccggatgcg tgagtatgta cgtggatgga ttacggaatg ggatttgcga cgattgtacc
      241 ctgaagctca attagacatt gaaattctgg atgtgcggca aacgtatgac gaggatcagg
      301 agctggaacc cacgatggcg gatgacattg gcaatgaaaa ggtatcagag tcggaagaaa
      361 gcttgatggc tgtcgcctta caaaatcaag ctcctgtgtg agtgagcata tcagcttggg
      421 ggaatcggcc gtatggcgca aaggccaccc tcctgccgag gaatacatag cttaatgtta
      481 gtctctgttg tgcttcccct cctttctctt attgagggtc cgcaaatata tttgaccgga
      541 tgggtaccca tcatcttctg aatagtggga gcccattggg agtcggattc aaccgtaatc
      601 ccttcgagaa aaatcgcaag ccaaagtctt ccgggccatg gggatgagag agtctctcat
      661 gggaggaatg ctcaattttg ttgctaagga tccgatagcg taagtcagag gaccaccctc
      721 gaagagggag gggattggaa aagccagcct tgagaagagg aaagatcttt gtcggtatct
      781 gagttgacgc ctcgtaaaga attgccgttg tcggttttgg gactatggcg cacctgtgtg
      841 aacagaatcc tgggagccat ggagctccta catcggcata cgattttggt gttaaccgtg
      901 ctttttgggc tctgtctcat cgtgatttat ttgcatattt cccaattgaa aactcaaatg
      961 gtgacggcca tggcttttcg gggatccgag atctatacca gagcgctgaa tgaaatgcgg
     1021 atatggtaca ccgtcgaagt ggttcaacgc cttcataacc agggcattga agcctcgaca
     1081 gggtaccggg accatcaagg cgaaattcct ctccctgcga ctctcagtcg tgacctgggt
     1141 aatcgattgg tcgctgggcg aaaaggcgaa cgattggagt tactgagccc ctatccgttt
     1201 tatcctggaa agaaatcagg aggacccatt gatggcttcc aggaagaggc gtgggaattt
     1261 ttcatgcaac atcctgatac ggatgagcca ttttatcggt ttgagcaagt cgatgggcgt
     1321 gagtccctcc gtttcgtgaa ggccgatcgg atgcagaagg aatgtgtgga gtgtcataac
     1381 agtcatccgg acagtccaaa gcgtgattgg cagatcgggc aggtgggcgg cttggtggag
     1441 gtcgtgtatc ccgttgattc gttggaaacg atcatgggta tgagttggca agggacgtat
     1501 gggttgatgg ggatgttggt cgtcatttgg atggggggat tcgggttagt ggtgttaaag
     1561 ctcggacggg tggccagtga attggaagag caggttcgtg atcgaaccgg cgccctgcaa
     1621 agtgccaaca aacatttgga attggaaatt catgatcgac tattggccga agataccctt
     1681 cggaaggcga aagataatct ggaaaagcga gtccaggagc gaacggggaa gttggctgcc
     1741 tcaaatgccg aactcattcg ggaagtggct gagcggaaac gggccgagga ggacatacgc
     1801 aaactcaatt cccagttggt ccaacgctcg gcccaactcg aggcgagtaa taaggaactt
     1861 gaggcgttta gttattcggt ctcccatgat ttacgggcgc cgttgcgggg gatcgatggg
     1921 tttagccagg ccgtgttgga agattatgat gaaaaacttg atgagtcagg acgaaattat
     1981 ttgcggaggg tgcggacggc cagccaacga atgtccaagc ttatcgatgc catgctcaat
     2041 ctggcccggt tgacacgagc cgaaattcat acccagacct ttgatctgag tgctgtagcc
     2101 cgggctgttc ttgaagactt tcaaaagatg gatccggacc ggcaggtcga atgtattgtg
     2161 gccaataatg tattcgccac cgctgacccg caattgcttc gggccgtttt agaaaatttg
     2221 atcggcaacg cgtggaagtt tacccagcaa caaacccacc cccgtatcga attcggttat
     2281 ggccaataca aagggcaggc agcctacttt gtctccgaca atggggcggg ttttgatatg
     2341 acctatgcgc ataaactctt cggcgcgttc caacggcttc atgcctatac agaatttccg
     2401 ggagtgggcg tggggctggc gacggtgcat cgtattattc agcggcatgg cggtcaaatt
     2461 tgggcagaag gagtggtggg caaaggggcg acctttcatt ttacgttgta aggcaaccgg
     2521 aggaggagaa gatgatgacc aacaaagtaa ttctgttagt ggaagatcat cctgatgatg
     2581 aggagttgac gatgcgggcg ctcaagaaaa acaatataaa aaatgaagtc gtggtggctc
     2641 gggatggcgt agaagcgttg gaatatctat ttggaaccgg agcgcatgcc ggtcgtgatt
     2701 taagccagat gcctcaaatt atcttgctgg atctcaagct ccccaaaatt gatgggctgg
     2761 aagtgcttcg gcggttgcga gctgatgaac ggacgaagtt tctcccggtg gttgtgctca
     2821 cgtcgtccaa ggaagagcag gacattgtaa aaagctatca acttggagcc aatagttatg
     2881 ttcgcaagcc agtggacttt caagagttct ctcaagcggt tcaaaacctg gggctctatt
     2941 ggctgatttt aaatgaatcg gcaccggaaa gggtgtgatc ctggcaggaa aaaggattac
     3001 ccacctcagt ggggggcgag tgtctgccct gggaggctcg gtggaaagtt ccggcatttc
     3061 ctggtctcgt tcattgacca accttctcat gggtgttgag tatttccgct cccattctct
     3121 ctgtcaaaga ttccaacagg tgtcaaaatt ccagtgactt cagacaggaa agatatgccc
     3181 gtccaccgca taacctgcct atcttcctga gccccgtctt ctggtcctta aaactcttat
     3241 cttttgtttc catacgcttt tctcttgtcc gactttccgg agtgatacca ggcatatccc
     3301 ttgctcagtg taagagttac tgatctaccg accattcttt ttaaggaggg ggccatgaga
     3361 cgaatgctga catgggatat tcccgaatcc gtcacgtcac atgaacatgc gtggaatcca
     3421 aaacgaaaat atatggctga aagctttggt ctggagaccc agcgggcgtc aagaagcagt
     3481 gcggccgggg ggatctgttg tgccaaatgt ggtagtgagg tgaaggtctt tgtttctccc
     3541 agacgaaatc cccattccag taaattaggc cgggctgtct cattgaagga tcatgatctg
     3601 tgccggcggt gttggcggaa attgatgcat gaacaacgga agacgggggt ggtgaccctg
     3661 cccatttcct tctttgtgaa agaggaaaat ctccggcctc gcctgttgat acccaaaatg
     3721 gcttccgtac ccaagcaagc gtcctaaccc tcgacataaa gagggggcga ggaatagcgg
     3781 taaatggctc aacctccaat gctaggtcgg atatggtcta tcagtgtgaa tttatttgga
     3841 ggaaatggtc gcttgtctct cttgaataaa tgctttcgca agggcagcat tttgatacac
     3901 gacctttaac tccaggtttc cgaacgggcg gcgcacgacc ttcagtaaca cctgtccatc
     3961 ttcaaaacct gaattcagct cgtcgagttg tccagcaaga aacgattgct tggtcgcttc
     4021 agcgagatcc tgcgtgaggt gataactcgc tccaagaatg atctgtaact gctggtacca
     4081 ttcatctcta tatagcccca tatcgatggt aatctttgcc agtttccctg ttggttcagt
     4141 cgtatctgag acctcttcgg gtaaataggt cagttcaaca tatcgatttt tatcggtaag
     4201 ttcatagaga cattcctttg agttgctgca ggctggtagg gcggatacct cctgttctgt
     4261 catgccaaat tttaattccc ggaagccatc aattccgggc tctgccaatc cgagcccacc
     4321 cgataggact attgaagcgc cggtgaagag cgcacaggtt cgtatgctta ggatgccgga
     4381 aagtaatttc ataacatgag gttccttact cgtaacgcta gagaaatgaa gaaaaaaaga
     4441 actatccagg tgatgtcggc tgttctgcat gaatcaatca ccatagcaaa cctttcctga
     4501 aaaaggcgat gctgcacggc aggaaaacta acaattgtgg ccatatcagg agtttggtta
     4561 ctcagaaaag aaattgttga ggggagactg tgaaatcaat ttggaaatgt gacgggcatc
     4621 gaattcattg ggagcattgg ggccttcctg gataggttct ctcaattgac ggtgcatgga
     4681 atacctcttg tccagagttt gatacttctc atgctcttcc tccttgcgat atctggaatg
     4741 cccatgccgg atcagcgtgt gagggctgag taagcgatgg tcctacccgc catggagcct
     4801 atttcaagtg aagctacagg cgagagaaaa cacgattttt cactggcagg gtcttgttgc
     4861 acctgcctca agttgtgtca gggagtttct tgatgaacct ggagaagtgg ccccatccag
     4921 taacgcatcg gtcgatcccc ttccgctgcc ttatgaggaa gatgggccat ataccaaaag
     4981 accacattgg tctcttggac gctttcatca cttttaaatt ccaaatctcc acgtgcgccg
     5041 aatggccagc cggcatcttc atcccggtaa aattttcgaa tcgagacatc acgatctgaa
     5101 aacttcttcg attctccatc aaatggacct ggaataaccc agacaccctg cccggtgggg
     5161 taatcgcgca caaaccagac gcgatcctga ctgttgttgc cgggcttctt gtcatcctcc
     5221 tctaccaaat atttttccca ccccggtccc caacccttgt cttttacaga attccggtcc
     5281 aggacaaaga cctgatcctg atcggatccg ccgatatcaa agtccagccg ccagtaggca
     5341 tggtgttcat ggtcggtatt acaggagaga ttccagctct gaacgcttgg atacaggcga
     5401 ccgtcttcgc tcagatacca ggcctgatac agatgatatt gaccgatttt ggcatacaca
     5461 cctaattcca accacttaac gccttggatg gtatgttcgg cctgacagag ctttttccca
     5521 tcgcagttag gatttttttt gaggcgctgt gggctgatcc ggtcctcgaa tggtccgcat
     5581 cggccactgg atcgccctag gccaaaccaa ctgaagggat gccaggccgg ccactcccga
     5641 acatacttga cccggatcac cggcaaatcc gctttaccta aaatctggtt gccttgatag
     5701 gacacattgc gtagccccag gccggcggca tctttcatct caaggtataa ggcccacgga
     5761 ccccaggtaa acggtttgga ttcccagtcg gctaaagcgg ggctgaccat caaagcactg
     5821 gtcaatgcga caccggcgag aaaatatcga aatgatgcca tgcgacgcgc tccttgtttg
     5881 ggaatagtca ttggggtcct gcctccttgc ctgcgtctaa aacggtctga gaagtgaggt
     5941 caactcgggc aaagtagaga gccaagctct ccggcgtttc agaaaatgtg acccaaaata
     6001 cccggtcccc gaaccccgct tcgtcgctca cccagaagta ccgatattct tcaggggaag
     6061 tcagaatggc atgtccatgc agatgctgaa cctcctgggc aattcgttga tctactctgg
     6121 ccagctcaat ggctgtggtg atttcttctt caccttcttc gggctggtag ccttcacgcg
     6181 gatcctgctg gacagaagac attcggtcat tttccatgca gacatgcacg gccacattgt
     6241 gggtgtaact atagtaggtt aaccgatatg cggaggcatc ggaagccgat ccccgctctg
     6301 tcgatcgagt gtgagtcgtg ttgtctgcct gcagggtcac caggcattgg tcggcaggga
     6361 cttcctccgt atcaatatag gcaaaacgat cgcccaattg ggtcttcacg tccggatccc
     6421 gttctgctgt ggcacgtaag gcaggctggt cggattcgac aatgataatc tccggtcccg
     6481 gaccggataa ggtcgtgggg gacatggcat caacggggcg tcgtggcccc ccttccggtg
     6541 gcaattccaa tggtaattca atcagaggag ggggatggaa gggcaggggt tttttgggga
     6601 tctttgttag ttccacgctg gagcatccca gaaaaaagct gcccaggacc caacccgccg
     6661 tcaccaggat atgcgaagtg aattttgaat gcatgaggcc ggctccttag gtcgaaaatg
     6721 aaaagcccta tagaaggaat tatttaacaa gcctacctta aaaattctcc ataagaatag
     6781 caagagagtc aggatagttc ccctaaaggt ggaaagggaa atgtttgaga ggtagtgtgt
     6841 ccatgggcat aataagcggt agaaagtttc ctcgtacgaa ggaatgaaag gagaacgtga
     6901 aatgggcata ccaatgaact ggtatctatg atgcgcgttt taataaatag atttttaagg
     6961 atttgcgcag gcagcccatg ccaacgaatt acagaattag tatggtctac caaaaaaata
     7021 ggtggtccct gaagaaaata catggatatc tttgaatgca tttatgcctc ttaaggaatg
     7081 tcggcttaca ggagagtctt tgcgggcatg gaggagacac cggggggagg gtttaaaaac
     7141 ataggaaaag aagttggcct tttggatcaa tattgattat gtcatggctg gttaaatcgt
     7201 gttgaatcat gatcggtcaa gagcattaaa gatgccgagt gattgttggc tttagtaggg
     7261 acattaattc taggcaccat gctttccgga ttcctatcgg gtcttcgcca caggcgactt
     7321 ttgggtattt acctcacgaa caatcacgcc accatcatta gctatgcaga gaactagcag
     7381 tgcggcccgc aggccgttat ctacaattct cccgtcaaga tgtctttaca atattcacct
     7441 gggatcgagc tttcagattg gggatccttt gcacataaga ccaaacgggg ctgagaggaa
     7501 ggggactcgg ttatcctgac gactgcgcac catcaaaagt actcttgtct caatatgcga
     7561 tgcgactctg tttttgatta cgttaccaaa attctgtaat gtcggcaggc taaaactatt
     7621 ttttatatta accaaaaacc aagaaaagat tcgacaatga cttctcgtct gactcatcgt
     7681 accttgctat ggttctcttt gattctctta accattttcg gcttttttct tatcgacgtc
     7741 ggattgtatt ttttgatacc tccaaaatgg gtccagtttg ctccagatta tcggttgcat
     7801 ccgaaaaaaa cacctttggg aggactgggc aaaggggtct cacgttatta ttatcaggcc
     7861 gaccctcttc ttggttttga tattacccct cttgctaagg gaacccattt tatactggac
     7921 ggggtggaga atgaaatatt tgcgaacgaa ctggggtgtt tcgatagaaa cagtctggaa
     7981 gatttcgttg ctagcaaaaa agactatgtt tattttgcgg gggactcttt tacctggggt
     8041 tttgcacgat atgaagacaa atttgcgaca gtctgggaaa ataacactgg aatgttaacc
     8101 gccaaatgtg gagtgggcca tacagggaca atacaccaat ttggtaaatt caaaaagatt
     8161 attgaaacaa ttgggcgtca gccgaccgtc gtgtttgttg gtttttttcc taatgat
//
LOCUS       DATIAL010000054         3384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_655251, whole genome shotgun sequence.
ACCESSION   DATIAL010000054 DATIAL010000000
VERSION     DATIAL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655251"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VLA60_01805"
     CDS             <1..1004
                     /locus_tag="VLA60_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HSF08114.1"
                     /translation="DGAYKRPRELMWIVGVILLAIMLAFGFTGYLLPWDQNAYWATQV
                     GINMVGSVPLIGDFLVKALRGGETLGALTISRFFAIHVAFLPLIIAVGIMLHLFILRR
                     VGPAGPHDETRAMAGSETFYPRQVFMDAVVMLGVFGVVAMLAISVEFPLADRADPSDH
                     SFVPVPEWYFLFFYQLLKYAPGAWGPLATWLLPTLFFTGLLLLPFVDRNPERHPSSRR
                     VVLGAGLGFLIVVFSLLSISLRDLYAVPKRDPSVIRGMALVDQFHCKTCHRIHGEGVN
                     VGPDLSFVADRRPDREWHLQHFKDPQSVSPGSFMPKFPLNEKQLNDLTNYMLTLKSG"
     gene            complement(1220..1930)
                     /locus_tag="VLA60_01810"
     CDS             complement(1220..1930)
                     /locus_tag="VLA60_01810"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HSF08115.1"
                     /translation="MPAVVAALQALEIEVMTGPATDMLNRIMATIPDIFAAAIILGIA
                     FAMSRLVSHLMSNLLGGLGFDTLPDKIGLGQAFSGQVTPSALVGKITVFFIMLFAIIE
                     AASQLGFEQVSVLITMFIEFGGQVLLGSAIIAIGFWLSNLAYETIIRIHGANSGGVAN
                     IARFAILGLVIAMGLRAMGLANEIVNLAFGLTLGAIAVAVALSFGLGGREAAGKQMEH
                     WLTGPWRTSRVEPFPQRL"
     gene            complement(1943..2710)
                     /locus_tag="VLA60_01815"
     CDS             complement(1943..2710)
                     /locus_tag="VLA60_01815"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08116.1"
                     /translation="MDMSKLTETLQATLEATLPSVMGALGILVLGWFVAMAVRAGVRR
                     GLGMLKVNQRLRSTTGAEMDIEGGTAAGIYYLILLLVLVAFFNALHLELVSGPLRALV
                     DQVLAFVPKLVAGGILILLVWIIATVVRTIVAKALQATTLDEKLSAGAGMRPISENLG
                     NVVFWLIVLLFLPAILGTLEIVGLLEPVQDMVNEMLGMVPNLFGAVLIGVVGWFLAKI
                     VRDLVSNLLTAVGTDRLGEQVGLRGTMSLSYLVAWVV"
     gene            complement(3157..>3384)
                     /locus_tag="VLA60_01820"
     CDS             complement(3157..>3384)
                     /locus_tag="VLA60_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HSF08117.1"
                     /translation="AVTMNYGVFLNSVISFLIVAFAVFMVIKAINKMKREEKEAPPAD
                     PTTKECKFCHMTIPIKALRCGHCTSELAKSA"
BASE COUNT          869 a          952 c          754 g          809 t
ORIGIN      
        1 ttgacggtgc ctataaacgg cctcgagaac tcatgtggat tgtgggcgtc atcctgctcg
       61 ccatcatgct ggcgttcgga ttcacgggat atttgctacc gtgggatcaa aacgcctatt
      121 gggcgaccca ggtgggaatc aacatggtcg gcagcgttcc ccttatcgga gatttcctgg
      181 tgaaagccct tcgtggcggg gaaactctcg gcgccctgac catatcacgt ttttttgcca
      241 ttcacgtcgc gtttcttccc ctaatcattg cagtgggaat catgctccat ctctttattc
      301 tcagacgggt gggacccgcc ggccctcatg atgaaacgag agccatggcc gggagtgaaa
      361 ccttctaccc acggcaggtc ttcatggatg cggtggtcat gcttggagtg tttggcgtcg
      421 tcgccatgtt ggcgatcagc gtggaattcc ctttggctga tcgggccgat ccgtcagacc
      481 attcatttgt tcccgtcccg gaatggtatt ttctgttttt ttatcagctt ctcaaatatg
      541 ccccaggcgc atggggacct ctggcgacct ggcttcttcc cacactcttt ttcaccgggt
      601 tgctgctcct gccctttgtg gatcgaaacc ccgaacgaca cccatcctca cgtcgcgtcg
      661 tgttgggtgc ggggttgggg tttttgattg tggtgttcag cctgttgagt atctctctcc
      721 gtgatctgta cgccgtgccc aaacgtgacc cctcggtgat tcgaggcatg gctttagtgg
      781 accaattcca ttgcaaaacc tgtcatcgta ttcacgggga aggtgtaaat gttggcccag
      841 acctttcttt tgtcgccgat cgacgacccg atcgagaatg gcatcttcag cattttaagg
      901 atccgcaatc cgtttccccg ggctctttca tgccaaaatt tccactaaac gaaaaacagc
      961 tgaacgacct caccaattac atgctgactc tcaagagtgg ataattcttc ttcctaccgt
     1021 gtaaaaatcg gttagttttg ttgaaagcca ccatcagctt agtgccctct gctgcgaacg
     1081 ctgctctccc cattgaggag tggatcagtc acgataccgg cgacaggtta tggccgattg
     1141 cggggtaagg gcaatccacc ctatacccca ttcgcaggca catcccgact gaacaaaaag
     1201 tgtcccccac gggcatggac tacagccgtt gggggaaagg ctctaccctc gacgttcgcc
     1261 acgggcctgt gagccaatgc tccatttgct tgccggctgc ctcccggcct cctagaccaa
     1321 aggataacgc caccgccacg gcgatggctc cgagagtcag gccaaatgcc agattgacga
     1381 tctcattggc caaccccatg gctcgtagac ccatggcgat cactaacccg agaatcgcga
     1441 agcgagcaat attcgccacc cctcccgaat tcgctccatg aatgcgaata atggtttcat
     1501 aggcgagatt cgataaccag aacccgatgg caataatggc actgcccaat aacacctggc
     1561 ctccaaattc aataaacatg gtgataagta cggatacctg ctcaaatcct aattggctgg
     1621 cagcttcaat aatggcgaat aacatgataa agaaaaccgt aatttttcca actaaagcgg
     1681 acggtgtgac ttgacccgaa aatgcctgtc ctaacccgat cttgtcagga agagtatcaa
     1741 acccaagtcc gcccaataaa ttactcatca aatgcgacac taaccgggac atggcaaacg
     1801 cgatccctaa aataattgcc gccgcaaaga tgtcgggaat cgtcgccatt attctattca
     1861 acatatcggt agcaggtccc gtcatcactt cgatttccaa ggcttgcagg gcagccacca
     1921 ccgccggcac caaaatcaga atttacacca cccacgcgac cagatacgac aatgacatcg
     1981 tcccacgtag cccgacctgt tcacccaatc ggtccgtgcc gacggccgtc aacaagttac
     2041 tgaccagatc cctgacgatc ttggctaaaa accacccaac aacaccaatt aagaccgctc
     2101 caaacaaatt cgggaccatg cctaacattt cattgaccat gtcttgaacc ggttctaaca
     2161 atccaacgat ctccaacgta cctaaaatag ctggaagaaa gaggagaaca atcagccaaa
     2221 atacgacatt ccccaaattc tcactaatag gccgcatccc ggctcctgcg ctgagttttt
     2281 catccagtgt cgtcgcttgc aacgccttgg ctacaattgt tcgaaccaca gtggcaatga
     2341 tccatacgag gagtattaat attcctccag ccactaattt cgggacaaag gctaagacct
     2401 gatccactaa tgcccgcaat ggaccagaga ccaactccag atgcaaagca ttaaaaaacg
     2461 ccaccaagac caacaacagg attaagtaat agattcctgc cgctgtcccg ccttcaatgt
     2521 ccatttcagc ccctgtggtc gaacgcaagc gctgattgac cttcagcatg cccaatcctc
     2581 ttcgaacacc ggctctcacg gccatggcca caaaccatcc caacacaagg attcccaagg
     2641 ctcccatcac gctaggaaga gtcgcttcaa gagtcgcttg cagggtttca gtcaatttac
     2701 tcatatccat agatcactcc tttcgttaaa ggtaagaagc caaggcgaaa atcagcctag
     2761 gtacccaacc tgccggtatt ttccgcaggc agacggttca acaaaaaatt tatggccgat
     2821 ttcgggagag aatacgaaag ggagttatag attcagcaca gagttcaaca ctaacaaagg
     2881 ggggaaagta ggtccacctg aaagccatat tcctcacatt ctggaaaatt tcgttcaacc
     2941 tgacatactg gaatgtctga ttactggaat atcaattttt ggcatataca agacggcatt
     3001 ccggttaccg ggaatgccgt ctttcggact cgccatgtat gaagaacccc cacctcctgt
     3061 tgatgatcac ccggttatta atccttgtcg gactatccgt tcaacgcaca tcagcatgtc
     3121 tgtataccat gtttcagggc atatcatcac gagcccttac gccgatttgg ccaactccga
     3181 ggtgcaatgg ccacaccgta gggccttgat gggaatcgtc atgtggcaga atttgcattc
     3241 cttcgttgta ggatctgcag gcggggcctc tttttcttca cgtttcatct tattgatggc
     3301 tttaatcacc ataaaaacgg caaaggccac aataagaaag ctaataaccg aattaagaaa
     3361 tactccataa ttcatggtga cagc
//
LOCUS       DATIAL010000055         5363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1516038, whole genome shotgun sequence.
ACCESSION   DATIAL010000055 DATIAL010000000
VERSION     DATIAL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516038"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VLA60_01825"
     CDS             <1..634
                     /locus_tag="VLA60_01825"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HSF08118.1"
                     /translation="DTLPLIMGGGPDPLPEVHRGYREGIERALVKLRTLKEIFEERIA
                     DAPQATAPVAAQPTKKAGGSRRVSVVHGRDDGTKETVARYLSKLDLTPIILHEQPNQG
                     KTIIEKFEAHADVDFALVLFTPDDVGHPAGEPDKARSRARQNVVLELGFFISALGRHR
                     VCVLYKGDIEMPSDYAGVVYVPLDVGGAWRLVVAREIKSAGIDVDLNKAM"
     gene            755..1465
                     /locus_tag="VLA60_01830"
     CDS             755..1465
                     /locus_tag="VLA60_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08119.1"
                     /translation="MKSTTKDLRYLNAMNKFLEYAFLALGDHFDSKDAFLRYFEAIEG
                     DFEKSHFLRTASFYLFMVKAGDWVVDVPGSDAKVEYLTNTHKYIAIFSLIESLLAEDH
                     IDFYQYLLGKKSKVEFPIENKEALTDLYQSYKSEFGAVSQCVSFFRSLSTQRQQDLIA
                     KLEVRGAEASLENLAKFMYELRSKFVHEGELVHHMSDGTSISFNGQKVFTCKLTIKDA
                     LIFFEEGLLAHFKKKGST"
     gene            1567..1911
                     /locus_tag="VLA60_01835"
     CDS             1567..1911
                     /locus_tag="VLA60_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08120.1"
                     /translation="MQSCIFQVETIGDGPAGLAPMPASLGKDELYPKKGTGAFVISFC
                     AGVRPGSRGPFVSAKGPKTIVAVAWPFGCPTSFADFGGAQTRYAQTMRAFPRIRLHDL
                     ASPQGHGIPMMP"
     gene            2454..2693
                     /locus_tag="VLA60_01840"
     CDS             2454..2693
                     /locus_tag="VLA60_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSF08121.1"
                     /translation="MPSEQLLTRITIDPQICHGKPCIRGLRYPVDWLLELMSSGMSNK
                     QILGDYPDLEEADLQAACAFGARLARVQRIEPVVG"
     gene            2690..3043
                     /locus_tag="VLA60_01845"
     CDS             2690..3043
                     /locus_tag="VLA60_01845"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HSF08122.1"
                     /translation="MKFLVDAQLPQHLAKELTRAGYDTLHTLDLPHGNRTRDEELTAL
                     ATREDRILITKDSDFVTTFHLQHSPPKLLLVSTGNIDNAALLQLFLTNLVHLERALIE
                     NDFVELGRTALTIHG"
     gene            complement(3392..4306)
                     /locus_tag="VLA60_01850"
     CDS             complement(3392..4306)
                     /locus_tag="VLA60_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HSF08123.1"
                     /translation="MSWKLLYWILGLIVLLPLFNFLLGVHPPRFQTDDDPKRYGLDYE
                     SVSFPTSDGLTLRGWFIPAASAARESLRQGGMAWNTCGTVLVGHGYPFDKANILRHAL
                     FLHPRFHLLLFDFRYFGESDGAYTTAGLLEIRDVQAAVEYVKSRSDVNPEQIGALGFS
                     MSASAFIMARHPDVKAIVADSPYGTLEGLIARQFFFFPGFTKWPLVALTKFYARLFLG
                     VQVNDAAPAEVVRDLNTPLLIIHGDADSQIPLEHSKAIYANADPEKTTLWIVRGADHG
                     FAHGLEGPRYEVRVREFFEQHLCPAAHT"
     gene            4833..>5363
                     /locus_tag="VLA60_01855"
     CDS             4833..>5363
                     /locus_tag="VLA60_01855"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HSF08124.1"
                     /translation="MNTFNKLRQDMIDQQLVARGLHDQRVLNAINAVPREEFVATDLV
                     EFAYSDSPLPIEASQTISQPYIVALMTAALELKPEDRVLEIGTGSGYAAAILGEIAQE
                     VYTIERHKILVDTARKRLAELGYRNIQVLYGDGTLGWPEHAPYDAIVVTAGGPGVPQP
                     LKNQLAIGGRLVIPVGS"
BASE COUNT         1351 a         1438 c         1373 g         1201 t
ORIGIN      
        1 agatacgctg ccactcatca tgggcggagg ccccgatcct ttgcccgagg tgcatcgggg
       61 ctatcgggaa ggtatcgaac gagccctcgt gaaactcaga acccttaaag agatttttga
      121 agagcgtatc gccgatgcgc cgcaggctac ggcacctgtg gccgctcaac ctactaagaa
      181 ggcgggaggc tccaggcgtg tttccgtcgt ccacggacgc gatgatggga cgaaggaaac
      241 tgttgctcgc tatctatcga aattggatct tactccgatc atccttcacg agcagccgaa
      301 ccaaggcaag acgatcattg agaaattcga agcgcacgcc gacgttgact ttgctctcgt
      361 cctatttact ccggatgatg tcggccaccc cgcaggggag ccggacaagg caagatcgcg
      421 agcaaggcag aacgttgtgc ttgagcttgg ttttttcatt tcggcgcttg gaaggcaccg
      481 cgtctgcgtg ctctacaaag gagacattga aatgccttcc gactacgcgg gagtggttta
      541 cgttccgttg gacgttggcg gcgcttggcg acttgtagtc gcgagagaaa tcaagagtgc
      601 aggcatcgac gtcgatctca ataaggccat gtagcagatg gaccggtcta acaacctaat
      661 caacccggac gcgcgcggaa acctggcgct atgcatgagc caaagcgcgc gcgggttact
      721 gggaacgtta tgtgaatagc gaaagaagca aagaatgaaa agcacgacca aggaccttcg
      781 ctacctcaat gcgatgaata agttcctaga atatgctttc ctcgcattgg gtgatcactt
      841 cgactccaaa gacgcctttc tccggtattt tgaagctatt gaaggcgatt tcgagaagag
      901 ccactttctg agaacggcct ccttctatct gttcatggtc aaggccgggg attgggtggt
      961 cgatgtcccg ggatcagatg ccaaggtcga atatctgacg aacacacaca agtacattgc
     1021 catcttctcc cttatcgagt ctctgctggc ggaagatcac atcgacttct atcagtacct
     1081 gctggggaaa aagtcaaagg ttgagtttcc aattgaaaac aaagaagctt tgaccgatct
     1141 ctatcagtct tacaagtcag aattcggagc cgtcagtcag tgtgtctcat tttttcgttc
     1201 gctcagcact caacgtcagc aggacctcat tgccaagttg gaggtccggg gagctgaggc
     1261 gtccttggag aacctcgcta aattcatgta tgagcttcgg tcaaagttcg ttcatgaggg
     1321 tgagctagtt caccatatga gcgacggaac atcaatcagc ttcaacggcc aaaaggtgtt
     1381 cacatgcaaa ctgacgatca aggatgcact aatattcttt gaagagggtc tgcttgcgca
     1441 ttttaagaag aagggcagca cataaccagg gtatccagcg gattttctac gcactgcgca
     1501 ctccgaaaac tcgctgatac ctgtcgttag gcgtcacaga atgcagcttg ggggcgtggt
     1561 caggttttgc aatcatgcat ctttcaagtc gagaccatcg gagatggtcc ggccgggttg
     1621 gctcccatgc ctgcttcctt gggcaaggat gagttgtatc caaaaaaagg aaccggtgcc
     1681 ttcgtgatat ctttttgtgc cggggttcgt cccggcagcc gaggtccttt tgtttcggca
     1741 aaaggaccca aaaccatcgt ggccgtggcg tggcccttcg ggtgtcctac gtcgtttgcc
     1801 gacttcggcg gcgcgcaaac tcgctacgct caaacaatgc gcgccttccc ccgaatccgg
     1861 ctgcacgact tggcctcgcc acaaggccac ggaattccaa tgatgccctg atcttctaag
     1921 acgaatctcg gcaacatcaa aacttcgata gataccacgg ctagaaaacc cgacttccac
     1981 accttcccaa gatctgacgc cttccgcccg gcccggggtc ggacgctcag atcatccggc
     2041 gggccttgtc tgagccccgc gagttggccc gccctcctaa gactcgcgtc cgtcccatct
     2101 aatgtggccg gacggggcgt cactggtttt ggccactttt gccgaaacaa aagtggctcg
     2161 ttcgccgggg cgaaacccgg caataccaat acccacttgg accacatatt ccaacagttg
     2221 tagaaggtga tacccaagat ggattcacga atacatctgt cgggaatgac agaacaggca
     2281 ctacgtttgt gaaatggctt ttgagccaca ctgaggggtg ggaagacggg cactcagttc
     2341 aaacagacta ttcggcaacg agtatattgc catggaaagt ggagtcaccc ccattcgcag
     2401 gcatctgtct cacccttctt gtcagggtag tcgaagtggg gtattcttgc actatgccca
     2461 gtgaacaact gttaacccgc atcacgattg atcctcagat ttgtcacggc aaaccctgta
     2521 tccgtgggct ccggtatccg gtcgattggt tattggaact catgagcagc ggcatgtcga
     2581 ataagcaaat cttgggcgac tatccggact tggaagaggc tgatcttcaa gccgcctgtg
     2641 cgttcggggc gcgcctagca cgtgttcaac gaattgaacc ggtggtgggg tgaaattcct
     2701 tgtcgatgcg caactacccc aacacctcgc taaagaattg acgcgggctg gttacgatac
     2761 actccatacc ctagaccttc cacacgggaa ccgtacgcgg gacgaagagt tgaccgccct
     2821 tgccactcgc gaggatcgca tcctgattac caaggatagt gacttcgtca ccacctttca
     2881 tcttcaacac agtccgccca agctcttact ggtttccacc ggcaatattg ataatgcagc
     2941 cctcctgcaa ctgttcctca caaatcttgt tcacctggaa cgcgcgctca tcgaaaatga
     3001 tttcgtggaa ctaggccgaa cggcactcac tatccacgga taaagggtgc ggggtcaggt
     3061 cctccaaaca tctatatttt aagtcgcgac catctgagct ggtcttggtc ggctcccatg
     3121 cctgcttccc tgcgaagaat gacttatatc caaaaaaaat ggaccggcgc cttcgtgata
     3181 tctttttttt ccggggttcg gcccggcaat accaataacc acttgaacac aatagtttgg
     3241 aatactagcg cacaaccttc caggaccaga cattgattga aaaacccaag atggattccc
     3301 gataacacct gtcgggaatg acaggaaatg acggggaatg aaaaagcgga ggagccagaa
     3361 gttcacgcaa tccaccggcc ctacacaccc tttaggtatg tgcagcgggg cacaaatgct
     3421 gctcaaaaaa ttctctcacc cgtacctcat accgaggacc ctcaagccca tgggcaaagc
     3481 catggtcagc cccccggaca atccagagcg ttgtcttttc gggatcggcg ttggcataaa
     3541 tggccttcga atgctcaaga gggatctgcg aatcggcatc cccatggatg atcagcaagg
     3601 gggtgttaag atcccgcacg acttcggccg gggccgcatc attgacttgt acacccagga
     3661 agagccgggc atagaatttt gtcagtgcga caaggggcca tttcgtgaag cccggaaaga
     3721 agaaaaactg ccgggcaatg agtccttcca gagtgccata gggactatcg gccacaatgg
     3781 ctttgacatc gggatgccgg gccatgatga aggctgacgc gctcatggag aatcccagcg
     3841 ccccgatttg ttccggattc acatcgctcc gacttttgac atactcaacg gcggcctgga
     3901 catcacggat ttccaagagc cctgccgtgg tataggcacc atcactctca ccgaaatacc
     3961 ggaagtcaaa caggagaaga tgaaagcggg gatgcaggaa gagcgcatgc cgaaggatat
     4021 tggctttatc aaaaggatag ccatggccca ccaggacagt cccgcacgta ttccatgcca
     4081 tccctccttg tcgcaatgat tcccgtgctg cagaggcggc gggaatgaac caccctctca
     4141 gggttaaccc atcagaggtg gggaatgaaa ctgactcgta gtccaacccg tagcgcttgg
     4201 ggtcatcatc cgtttgaaac ctcggcggat gcacacccag cagaaaattg aacaatggca
     4261 agagaacgat aagcccaagg atccaataca gcaatttcca gctcacagga ttctcctcaa
     4321 tgcagaggta aatcctccac aaggaacatg aaagaagcga cttaacatat cccgaaattg
     4381 tgaccggaga ggattttggg gccagatcgc ctcgctcgac ttattatgat cttcataaaa
     4441 acatgtaaag gcagataatt tcgccaccag atgaaccgcc ttatgaaaca atcttagaca
     4501 agactactga gaaaaaaaac gactcccggt tccattgacc caaaggcttc ttaaggcagg
     4561 cctgtgtaga gatttgcccc acaaattttc gtggtgcgtc tcctatgcat gcctcaattc
     4621 acgaatttcg ccctagaaaa attttttaat tggctcttat tgcaggggaa cgggtcattg
     4681 ccttgattgc cgtacacagg taggcaacca cctggcacca ttattgcgga atcaattcta
     4741 cagaaccggg tggagaattt ttttcaatcc tttttttcaa tcccatgtga ctatcggcat
     4801 catgttaaaa aggacatcct caggagtcac acatgaacac gttcaataag ttacgacagg
     4861 acatgattga tcagcagtta gtggctcgtg gcctccacga ccaaagggtg cttaatgcca
     4921 tcaacgccgt ccctcgtgaa gaattcgtcg cgacggacct tgttgaattt gcctatagtg
     4981 actcgcccct accgatcgaa gccagccaga ccatttctca accctatatc gtggcgttga
     5041 tgactgctgc gcttgaatta aagccggaag accgcgtgct ggaaatcggg acgggttccg
     5101 gatatgccgc ggcgatattg ggggaaattg ctcaggaagt ctacaccatc gaacgacata
     5161 aaatactcgt agacaccgca cgcaaacgcc tcgcggaact gggttatcgc aacattcagg
     5221 tcctttatgg agacgggacc cttggatggc cggaacatgc cccctatgat gccattgttg
     5281 tcaccgcagg cggacccgga gtacctcaac ccctgaaaaa ccagttggcc atcggtgggc
     5341 gtctcgtgat ccccgtgggc tcg
//
LOCUS       DATIAL010000056         5062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1301032, whole genome shotgun sequence.
ACCESSION   DATIAL010000056 DATIAL010000000
VERSION     DATIAL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301032"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VLA60_01860"
     CDS             complement(<1..975)
                     /locus_tag="VLA60_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08125.1"
                     /translation="MDSIKFGTSGWRAIVAEDFTVRNIRIVCQGIAQFLRQQKLGQQG
                     ILIGYDARFLGEHFAKVAAEVMAAHGIPCLICDRDTPTPAISYHVVSRKLSGAMNISA
                     SHNSPEWNGIKFTPDWGGPALPETTKAIELRILPLLHGEHIKWLPWERATHDGMVRHF
                     DPQPEYLKSLEPLVDRDRIRNGRIRVIFDPLYGTSRHYLDEFLRRAGAKMAVLHHWRD
                     PYFGGFRPKPTDETLQDLKETVRREGAHLGLAIDGDGGRFGIVDADGTFIQANYILAL
                     LLDYLIRSRQWTGGVARSVATTHLIDAVAAHHGLTVHETPVGFKYIGEL"
     gene            complement(1251..2024)
                     /gene="xth"
                     /locus_tag="VLA60_01865"
     CDS             complement(1251..2024)
                     /gene="xth"
                     /locus_tag="VLA60_01865"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSF08126.1"
                     /translation="MKIATWNVNSIKVRIPHLMEWVKQANPDIVLLQELKTTEDQFPR
                     MAIEEMGYNMAIVGQKTYNGVAILSKAPIEIEHTALPGAPSDEQARYVEAVVGNVRVA
                     SIYLPNGNPVETEKFTYKLAWLDRLITHAQSLLALEEAVVLGGDYNVCPTDHDVYDPN
                     GFADDALCRPESRARFRALCHLGYTDALRALHPELGLYTYWDYQAGRWNRDEGLRIDH
                     LLLSPPAADRLVAADVDRGPRGMDKPSDHTPVWCELSPS"
     gene            complement(2465..2752)
                     /locus_tag="VLA60_01870"
     CDS             complement(2465..2752)
                     /locus_tag="VLA60_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08127.1"
                     /translation="MEIPTYKELLKLMWFLVWRHVVILAFVSMTIGWVLPVIFNNTVS
                     EGSQYLLEICIYGVLFFGITPFILKTLFQKQFQGFQVIFQRETDSPPLSSS"
     gene            complement(2979..3353)
                     /locus_tag="VLA60_01875"
     CDS             complement(2979..3353)
                     /locus_tag="VLA60_01875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HSF08128.1"
                     /translation="MTPQYGHVYLADLNPRFGTEPGKVRPVVVVQTDALNPYHLSTLI
                     CPLTTQVFDVQTPLRVSIPQGVSGLTAISDILVDQIKAIDNQRFRKELGALPDPYLAE
                     LRKKLLQILDFVEESLSLTPPT"
     gene            complement(3350..3592)
                     /locus_tag="VLA60_01880"
     CDS             complement(3350..3592)
                     /locus_tag="VLA60_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08129.1"
                     /translation="MSKVLSLKLDENAYQETEAIRKRLNMPRNLYIRKALAHFNALYA
                     RKALAQDYQKSSQQVSAAHLAYLKETELLDDLPGDL"
     gene            complement(3734..>5062)
                     /locus_tag="VLA60_01885"
     CDS             complement(3734..>5062)
                     /locus_tag="VLA60_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HSF08130.1"
                     /translation="KALEYAAEDADVTLRLHQVLKPRLISEHMTTVYETLERHLIPVL
                     ATMEQTGIKVDVSQLKQVSQEFSLRLRELEQEIHRRAGKTFNVGSPKQLGEVMFDDLA
                     LGGGQKGKAGSYSTGVDVLESLAAQGHELPSRVLEWRHLEKLRSTYADALIRQINPDT
                     NRVHTSYAMASAATGRLSSTDPNLQNIPIRTEEGRRIRRAFVAEPGWTLLSLDYSQIE
                     LRLLAHVADIPVLKKAFWEGQDIHALTASQVFGIPLEQMDPGIRRKAKAINFGIIYGI
                     SAFGLARQLSVEPGEAAAYIKTYFEQYPGIQDYMERTKQFCRQHGYVQTLFGRRCHVP
                     GIHDKNPAKRNFSERAAINAPLQGTAADILKRAMIRVPAALSKHGLTPKAKMLLTVHD
                     ELLFEVEEVAVKQTTSVIKQVMEAATLPALQLSIPLTVEAGHGQSWDEAH"
BASE COUNT         1166 a         1207 c         1397 g         1292 t
ORIGIN      
        1 caattcgccg atgtatttga atccgaccgg ggtttcgtgg acagtaagtc catggtgtgc
       61 cgccacagca tcaatgagat gggtggtggc aaccgagcgg gcaactccac cggtccattg
      121 ccgactgcga atgagataat ccaacaaaag ggccaggatg tagttggctt gaatgaaggt
      181 gccgtcggca tccacgatgc caaaccggcc tccgtctccg tcgatcgcca ggccgaggtg
      241 tgccccttcg cggcgaacag tctctttcag atcttgaaga gtctcgtcgg tcggtttcgg
      301 tcgaaatcct ccaaaatacg gatctcgcca atggtgtaag acggccattt ttgcaccggc
      361 tcgccggagg aattcatcca gataatgcct ggaggttcca taaaggggat caaaaatcac
      421 ccggatccgg ccgttccgga tgcggtcgcg atccaccagc ggttccagag atttcaaata
      481 ctcgggttgc ggatcgaaat gccgcaccat gccatcgtgt gtcgcccgtt cccaggggag
      541 ccatttgatg tgttctccat gcaacagggg tagaatacgc agttcaatgg ccttggtggt
      601 ttcaggcagc gccggtcctc cccagtccgg cgtaaatttg atcccgttcc attcagggga
      661 attgtggctg gctgagatat tcatggctcc tgataatttc cgactgacca cgtggtagga
      721 gatggccggg gtcggcgtgt cgcggtcgca aatcagacaa gggatcccat gggccgccat
      781 gacttctgca gccactttgg caaaatgctc ccctaaaaat cgtgcgtcgt agccaataag
      841 gatgccttgt tgccctagct tctgttgacg cagaaattga gcgattccct ggcatacgat
      901 acggatgttc cggaccgtaa agtcctcggc cacaatggcc cgccaccctg aagtcccgaa
      961 tttaatggag tccatgcgtg atgaatgctc gtcgaaatgg agcaggaatt tttcagcaga
     1021 aattaagcat tacaggattt gggtgttaat tcccaaaagt attacagaat agaagatttt
     1081 tcttcatttg ccaatcgggt agcgtgggat tttttttaag atgcctggaa aatttcctta
     1141 acgggctatg gccttagcta gaaggaaaga taaaaattgt tcggaaagga aaaaagaaaa
     1201 tgttgatctt tctgtataga tcaggctatg ccgaggcgat gtctcctctt ttacgaggga
     1261 gatagttcac accagacggg agtgtgatcg gaaggtttgt ccattccgcg cggtcctcga
     1321 tcaacatctg ctgcgactaa ccggtctgcc gctggtggag agagcaggag atggtcgatg
     1381 cggagccctt cgtctcgatt ccatcggccg gcctggtaat cccaataggt gtagagaccc
     1441 aattcggggt gcagggctcg aagggcatcg gtgtacccga gatggcagag ggcgcgaaag
     1501 cgggcacggg attctggacg gcagagggcg tcgtcggcaa atccatttgg atcatagacg
     1561 tcatggtctg tgggacagac gttatagtct ccccctaaca ccacggcttc ttccagggct
     1621 aagagcgatt gcgcatgcgt gatcagtcga tccaaccagg caagtttata tgtaaacttc
     1681 tcggtttcca ccggattccc attgggtaaa taaattgatg cgactcgaac gtttcccacc
     1741 accgcttcca cgtagcgggc ttgttcgtca gaaggggcac cgggaagtgc ggtgtgttcg
     1801 atctcgatcg gggcttttga aagaatggcg actccgttgt acgttttttg cccgactatg
     1861 gccatattat agcccatctc ctcaatcgcc attctgggga actggtcctc agtggttttg
     1921 agttcttgca gcaggacaat gtccgggttg gcttgtttga cccattccat caagtggggg
     1981 atccggactt taatcgagtt gacgttccag gtggcgattt tcatatgagt ttcagaatgt
     2041 gtattaagca aatattttga tcatagagcg tttaaggttc ttggtttatc atatgatggt
     2101 ttgaattcaa aggaatacat catggggggt tcagaaattt gttggaaggt gaagggattg
     2161 tatttgtgtt gccaaatctc gcccccacgg tatttcccgt attgccgggt ttcggcccgg
     2221 cagccgaggc ccttttgttt cggcaaaagg acccaaaacc agtgacgccc catctggcct
     2281 atgagagcgg agggacgcga atctgaggag tgcggcccaa ctcgctgggc tcaaacaagg
     2341 cccgccagtt gatgagagcg tccctccctt aggccagatg gcaggcgtcg gaccaggaga
     2401 gacgaatatt gcagtgacac accggaagga gaccgaaatc atcatatgct gaatgaaccc
     2461 aatattatga tgaagaaagt ggtggtgagt cggtttcgcg ttggaagatg acttgaaagc
     2521 cttgaaattg tttttgaaac agggttttca aaataaatgg cgtaatgcca aaaaataaaa
     2581 cgccataaat acagatttct aatagatatt gagagccctc agagacggtg ttattaaaga
     2641 tcacgggtaa cacccacccg attgtcatgc tgacaaacgc caggatcacg acatgtcgcc
     2701 atactaggaa ccacatgagt ttcaacagtt ctttatatgt tggtatttcc atatgactcc
     2761 tttgtcagca cagcgttcca gagggtaaca gatttatggg gagtgttgaa aaatgccgcc
     2821 agcggcgttc tcgccccttg gccgtgctca cgtactcctt catacgctcc gctcgtccaa
     2881 ggggctgcgg ccttgctgga cgggaatttt tgaacactcc cccaattttc catgtttgca
     2941 ttttggtcac ccatgggttt ctgaatatgg gatatgactc aagtgggcgg tgtcaaagag
     3001 agactttctt cgacaaagtc caagatttgc aatagttttt tccgaagctc tgcaagatag
     3061 ggatcgggta atgccccgag ttctttgcga aagcgttgat tatcaatggc cttgatttga
     3121 tccactaaaa tgtcagagat ggcggtcagc cctgagaccc cttgaggaat cgatactcga
     3181 agaggagttt ggacatcaaa cacttgagtg gtcaatggac aaatgagtgt agaaagatga
     3241 tagggattca aggcatcagt ttgaaccacg acaaccggtc gaacctttcc gggttcagtt
     3301 ccaaatcttg gattcaggtc cgccaaatac acatgaccgt actggggcgt cataggtcgc
     3361 ccggcagatc gtctagaagt tcggtttctt tcaaataggc gagatgggcg gcagagacct
     3421 gctgtgatga tttttgatag tcctgggcca acgcttttct cgcgtatagg gcgttgaagt
     3481 gagccagagc ttttctgata tacaggtttc gaggcatatt caaacgtttt cgaattgctt
     3541 cggtttcttg gtaggcgttt tcgtcgagtt tcagggataa cactttactc atcgtctttg
     3601 cctcctttta ggtattacca aaagtatata ccaacattct tgaaaagtga atccgtgcgg
     3661 gagaaacgta aggagtgagg cgaaaaatcc tccagtcgcc ttacccctta cgtttcacgt
     3721 tttccttctc cctttaatgg gcttcgtccc aggattgacc atgtccggct tcgaccgtca
     3781 gcgggatcga caattgtagg gcagggagtg tggcggcttc catcacctgt ttaatgaccg
     3841 acgtggtttg cttgaccgcc acttcttcta cctcgaataa taattcatcg tggacggtca
     3901 atagcatttt cgccttgggg gttagcccat gctttgacaa tgctgcaggt acgcggatca
     3961 tggcgcgctt caggatgtcg gcggccgtgc cttgcaatgg ggcattgatg gccgctcgct
     4021 cagagaaatt tcgtttggcg ggatttttat cgtgaatgcc cgggacatgg caccgacgtc
     4081 caaacaatgt ctggacgtat ccgtgttgcc ggcaaaactg tttggtgcgc tccatgtaat
     4141 cctggatgcc gggatactgc tcgaaatagg ttttgatgta ggcagcggcc tcgcccggtt
     4201 ccacgctcaa ttggcgggcc aacccgaagg cgctgattcc ataaatgatg ccgaaattaa
     4261 tggccttggc ttttcgtcga attcccggat ccatctgttc cagggggatg ccgaagacct
     4321 ggctggcggt gagcgcatga atatcctggc cttcccaaaa ggctttcttg agaacgggaa
     4381 tatccgcgac gtgggcgagc agacgcagtt caatctgtga atagtccagc gaaagcagtg
     4441 tccaaccagg ctccgcgaca aaggcgcgcc ggattcttcg accttcttcg gtgcgaatag
     4501 gaatgttctg gagattgggg tctgtggatg agagtcttcc cgttgcggca gacgccatgg
     4561 cataggaggt atgcacccgg tttgtgtcgg gattgatttg acggatgagc gcatcggcat
     4621 acgtgctgcg cagtttttcc aaatgccgcc attccaagac acgtgacggg agttcatggc
     4681 cttgggccgc taaggattcg aggacatcga cccctgtgct atagctgccg gctttgcctt
     4741 tttgaccccc tcccaaggcg aggtcgtcaa acatcacttc gccgagttgt ttgggggaac
     4801 ccacattgaa ggtctttccg gcccggcgat ggatctcttg ttccaattcc cggagacgga
     4861 gagaaaactc ctggctgacc tgtttgagtt gggacacatc gactttgata cctgtttgct
     4921 ccatggtggc cagcacggga atgaggtgac gctcgagtgt ttcgtagacg gtggtcatgt
     4981 gttcgctgat gagacgaggt ttgaggacct gatggagccg cagggtcaca tcggcatctt
     5041 ccgcggcata ttccaacgct tt
//
LOCUS       DATIAL010000057         5614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1301602, whole genome shotgun sequence.
ACCESSION   DATIAL010000057 DATIAL010000000
VERSION     DATIAL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301602"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VLA60_01890"
     CDS             complement(<1..613)
                     /locus_tag="VLA60_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HSF08131.1"
                     /translation="MPVEPRLTQVAIEDEMRLSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRILYGMNEMGLAAGKPYRKSAKIVGEIMGNYHPHGDSAIYDTLVRMAQDFNMRYPLV
                     DGQGNYGSVDGDPPAAMRYTEARLTKLAEEMLVDIDRETVNFTPTYDESSQEPIVLPA
                     RIPNLLVNGAGGIAVGYATNIPTHNLGEVVAALIALIERPEVTI"
     gene            complement(716..3220)
                     /gene="gyrB"
                     /locus_tag="VLA60_01895"
     CDS             complement(716..3220)
                     /gene="gyrB"
                     /locus_tag="VLA60_01895"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HSF08132.1"
                     /translation="MESAKNPSQESAPSADRQHSDARYDADQIRVLEGLEAVQKRPAM
                     YIGSTGVDGLHHMVYEVVDNSVDEHMAGYGNEIQVVLQVDGSVMVSDNGRGIPTGMHS
                     TQKKSAAEVALTVLHAGGKFEEGAYKVSGGLHGVGISVVNALSEWLELEIWQNGEVFE
                     QRYQRGKPQTPLTVAGITKKRGTKVIFKPDPLIFETVEFSFDVLAQRLRELAFLNKGL
                     EICLRDDRTGKEKEQVFCYIGGIVSFVEHLNEAKSPIHPPIVVEVEKPEMILQLALQY
                     NEGYSENLYSFANNINTREGGTHLIGFKSALTRTINSYATANDLFKKDSESLSGEDVR
                     EGLTAVVSVKIRNPQFEGQTKAKLGNSEVKGIVESAVNEALGSYLEENPSVAKKIVGK
                     SVDAARAREAARKAKELIRRKGALDGGSLPGKLADCSEKDPQFSELYLVEGDSAGGSA
                     KQGRDRRSQAILPLKGKILNVEKARFDKMLASEEIRTLIMAIGTGIGRPREDAEKGKE
                     DKDAFDINRARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPELIERGYIYIAQPPLFK
                     VKKGKGEKYLKDEAAMNTYLSNLAVEDTQLFLPEQNEFVTRDELIPILDKLVAFEGLL
                     TRQGQKQIEPALLRVLVDFPELTKDLLKNRGDLEVLIEEATRRLRLAFPEGTVDLTIQ
                     TDEEHQAHHILCRVAGNGSQKFLNLTHDMVGSADFRELQKIAPSALGLGRPPYRLKRK
                     EIETEFSTSQDLVTEFLETGKKGMSIQRYKGLGEMNPTQLWDTTMNPETRSLLQVTLE
                     DTTGVDEIFTILMGDEVEPRRNFIQKHALEVRNLDV"
     gene            complement(3277..4401)
                     /gene="dnaN"
                     /locus_tag="VLA60_01900"
     CDS             complement(3277..4401)
                     /gene="dnaN"
                     /locus_tag="VLA60_01900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HSF08133.1"
                     /translation="MKIQITREDLLTALQRVQGVVEKRNTMPILANILLEAKPEGLDI
                     VATDLEIGMRGLYKATVHEPGSVTFSARTLFDILKEIRHSDIELVVGENNWVTVKAGK
                     SQFRVVGLPSKDYPALPTIEREGLMALPAQGLLQLLKKTVFAVGEKDTRYVLNGLLLT
                     LIPSGATVTLRLVGTDGHRLAWAEQEVTPEKAAIPPAEIKVIIPKKAAIEIRRLLEED
                     DEQPFLGFTKNMLIFRKSGLVLTSRIMEGTYPNYQQVVPKESSKKVTVNKSDLEGALR
                     RVAILSKDKAHAVKLSINPDHIHLSSKSPDLGEADEDIPAKFVGESFSTGFNARYFLD
                     VLSVIETETLTLQMETPLSPCLIQEQGNPTFKAVVMPVKV"
     gene            complement(4424..>5614)
                     /gene="dnaA"
                     /locus_tag="VLA60_01905"
     CDS             complement(4424..>5614)
                     /gene="dnaA"
                     /locus_tag="VLA60_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HSF08134.1"
                     /translation="RNYKDLLKEAFQHVEGVNPAEIVFVMKEQDGDGEPSPQLKTEVR
                     ENRVPAQLQRVRRQPLPNPKYTFKNFVVGASNQFAHAACLAVAESPAKAYNPLFIYGG
                     VGLGKTHLLNSIGNYIADHGDLRIAYVTTEQFTNEVINSIRYDKMIELRRRYRNVDML
                     LIDDIQFLAGKERTQEEFFHTFNSLYEAGKQIVLSSDRFPKEMPSMEERLRSRFEWGL
                     IADLQPPDVETRIAILRKKSEEEGIAINEEVIQLLAANLKSNIREIEGALIRLGAYAS
                     LTGQKITADMAKHILRDLLGGKRKVITTEDIQEVVANRFHVKISDLKSKRRTKTLVFP
                     RQIAMFLSRDMTDSSFPEIGRDFGGKDHTTIIHACKQMQKAQETDSTLRATIESLKEE
                     IGKG"
BASE COUNT         1298 a         1477 c         1277 g         1562 t
ORIGIN      
        1 gaatggtcac ctcgggccgt tcaataaggg caatcagcgc ggcgactacc tcgcctaaat
       61 tgtgggtggg aatattggtg gcatacccga ccgcaattcc tcctgctcca ttcaccaaca
      121 gattcggaat acgagccggc aacacaatcg gctcttggga cgactcatcg tacgtcgggg
      181 taaagttgac ggtctcccgg tcgatatcga ccagcatttc ctcggctagc ttggttaatc
      241 gggcttcggt gtagcgcatg gcagcgggtg gatcgccatc aaccgagcca tagtttccct
      301 ggccatccac gaggggatac cgcatattga aatcctgggc catacgaacc aacgtgtcgt
      361 aaatggctga atcaccatgc gggtgatagt tacccatgat ttcgccgaca atttttgcgg
      421 atttcctgta gggcttcccg gcagccagac ccatttcatt catgccatac aggattcgcc
      481 gatgaaccgg cttgagcccg tctcgcacgt ccggcaacgc ccgcccgaca atgacgctca
      541 tagcgtagtc caaataggac aaacgcattt catcttcaat ggcgacttgc gtcagacgtg
      601 gttcaaccgg catacgtcac tcctcgaaag agatcatcac ccttcatgtt ctgcgcttta
      661 acttgattta ctctggctca acattcgggg tcacacaacc aaatgcaggc acctgctaga
      721 catccaaatt tcgaacttcc agggcatgct tttgaatgaa attccggcgg ggctcgacct
      781 catcacccat caaaatggta aagatttcat ccactcccgt ggtatcttct aaagtcacct
      841 gaaggagact ccgggtctct ggattcatcg tggtgtccca taattgcgtc ggattcatct
      901 cgcccaaccc cttgtatcgc tgaatggaca tgcccttttt cccggtttct aaaaactctg
      961 tcacgagatc ctgactggtg gaaaattccg tttcgatctc tttgcgtttt aagcgatagg
     1021 gtggtcgccc caatcccaat gccgaaggag caattttttg taattccctg aaatctgcag
     1081 atcccaccat gtcgtgagtc agattcaaaa atttctgact gccattccct gccacccgac
     1141 acaggatgtg atgggcctga tgctcttcat cggtctgaat cgtcaggtcc acggttcctt
     1201 cggggaatgc cagccgaagc cggcgtgtgg cttcctcgat caatacttcc aagtcgccac
     1261 ggttttttaa caaatctttg gttaattccg gaaaatccac taacacgcgg agcagcgccg
     1321 gttctatttg tttttgcccc tgacgggtta aaagcccttc aaaggccacg agcttatcta
     1381 atatcgggat gagttcatct ctggtcacaa attcgttttg ttccggaaga aagagttggg
     1441 tatcctccac ggcgagatta gacagatagg tattcatcgc cgcttcgtcc ttgagatact
     1501 tctcgccttt tcctttttta accttaaaca agggaggctg agcaatgtag atatagcccc
     1561 gctcgatcag ttccggcatc tgtctgaaaa agaaggtcag gagaagagtt cggatatggc
     1621 tgccatcgac atccgcatcg gtcatgataa tgattttgtg gtaacgagct cgattgatat
     1681 caaaggcgtc tttatcttcc ttgccttttt ccgcgtcctc ccgtggccga ccgattccgg
     1741 tcccgatggc catgatgagc gttcgaattt cctcactggc gagcatttta tcgaatcgag
     1801 ccttctcgac attcagaatc ttgcccttga gaggaaggat cgcttgcgaa cgtcgatcac
     1861 gcccctgttt cgccgatccg ccggccgaat caccctccac aagatagagt tcactgaatt
     1921 ggggatcctt ttcggagcag tcagccaatt ttcctggaag tgagccgcca tccaatgcgc
     1981 cttttcttcg aatcagttct ttggcttttc ttgcggcttc cctggcccgt gcggcatcca
     2041 cagacttccc gacgattttt ttggccaccg agggattttc ttccaggtac gatcctaagg
     2101 cttcattgac ggccgactcc acaatgccct tgacctcact attgcctaat ttggctttgg
     2161 tctggccctc aaattgggga ttgcggattt tcacgctcac tacggcggtt aacccttctc
     2221 gaacatcttc tccgctcagc gattccgaat cttttttaaa caaatcattg gcagtggcgt
     2281 aactattgat agtgcgcgtc aaggccgact taaatccgat gagatgcgtg ccgccctcac
     2341 gggtattaat attattggca aaggaataga gattctctga atacccttcg ttatattgaa
     2401 gggccaactg gagaatcatt tcgggttttt ccacttccac gacaatggga ggatgaatgg
     2461 gggatttggc ttcatttaaa tgttccacga aggagacaat ccctcctatg taacaaaaca
     2521 cctgctcctt ttctttgccc gtccggtcat cccgtaaaca aatctccaag cctttattga
     2581 gaaaggccaa ctcccgaagt cgttgagcca ggacatcaaa actgaattcg acggtttcaa
     2641 aaatgagggg atccggctta aaaatgactt tcgttccgcg ttttttggtg atccctgcga
     2701 ccgttaatgg agtctgcggc ttcccacgtt gataccgttg ttcgaagact tccccatttt
     2761 gccaaatttc caactccaac cattccgaga gggcattcac gaccgatatg cctacgccat
     2821 gtaagccacc cgagacttta taggcaccct cctcaaattt tccgcccgca tggaggacgg
     2881 tcaatgcgac ttcagcggcc gattttttct gggtggaatg catgccggtg gggatccctc
     2941 ttccattatc cgaaaccatg acactgccat ccacttggag cacgacctgt atttcatttc
     3001 catagccggc catgtgttca tccacactat tatcaacaac ctcatacacc atatggtgca
     3061 gaccatcgac ccccgtgctg ccgatataca tggccggtcg tttttgaacc gcttccagtc
     3121 cttccaggac ccggatctga tcggcatcgt accgagcatc cgaatgttgg cggtctgcag
     3181 agggggcaga ttcttggcta gggttttttg ctgattccat acaattttag gctttaactg
     3241 cgtgtggaag aaggaatgtc tgttgggtca aatccgtcaa accttcacgg gcatcaccac
     3301 ggctttgaac gtgggattgc cctgctcttg aatcaaacac gggcttaagg gtgtttccat
     3361 ttgaagggtc agcgtttccg tttcaatgac ggataacaca tccaggaaat acctggcatt
     3421 gaatcccgtg gaaaaacttt ctcccacaaa tttagccggg atgtcttcat ccgcttcccc
     3481 taaatccggg ctttttgaag aaagatgaat gtgatcggga ttgatggaca gtttcacggc
     3541 atgggcctta tctttcgaaa ggattgccac tctccgaagg gcaccctcca aatcggactt
     3601 attgacggta acttttttgg agctttcttt cggtaccacc tgctggtagt tgggataagt
     3661 cccttccata atccgggagg ttaacaccaa cccgcttttc cgaaagatca acatattttt
     3721 cgtaaaccca aggaacggtt gttcgtcgtc ttcttccaag agtcgtcgga tttctatggc
     3781 ggcttttttt ggaataatga ccttaatttc tgccgggggg atagccgctt tctcgggagt
     3841 cacttcctgc tcagcccagg ccaatcgatg tccgtcggtt cccaccaaac gaagcgtcac
     3901 ggtggctccc gaaggaatca gagtgagaag aagaccgttt aacacatatc gcgtatcttt
     3961 ttcccccacg gcaaagacgg tctttttcag aagctgcaac aatccttggg cggggagcgc
     4021 catcaaccct tcccgttcaa tagttggaag ggcagggtaa tccttactgg gtaagcccac
     4081 cactcgaaat tggctttttc cagccttcac cgtcacccaa ttattttctc ccacgaccaa
     4141 ttcaatgtcg gaatgccgga tttctttcaa aatatcaaaa agcgttcgag cggaaaatgt
     4201 gaccgacccg ggctcatgga ccgtggcttt atagagccct ctcatgccga tttccaaatc
     4261 agtggccaca atatccagac cttccggctt ggcttccaac agaatattcg ccaggatcgg
     4321 catagtattc cgcttctcca ccactccctg cacgcgttgg agtgcggtta acaaatcctc
     4381 cctggttatt tgaattttca taggtgtcct ataaacggtg gtgttatccc ttgccgattt
     4441 cttctttcag gctttcgatt gtggctcgaa gagtggaatc cgtttcctga gccttttgca
     4501 tttgcttaca agcatgaata atggttgtat ggtcttttcc cccgaagtct cttcctattt
     4561 ccggaaagga ggagtccgtc atatcgcggc tgagaaacat cgcaatttgg cgaggaaaca
     4621 ctaaggtttt cgttcgtcgt ttcgacttca agtcggatat cttgacatgg aaccgattgg
     4681 ccaccacttc ttgtatatcc tccgttgtga tcaccttccg ttttccacca agaagatcac
     4741 ggaggatgtg tttggccatg tccgcagtga ttttttgacc cgtgagagat gcataggcgc
     4801 caagtcgaat cagtgcccct tcaatttcac gaatattgct ttttaaattc gctgccaaca
     4861 actggatgac ttcctcattg atggcgatac cttcttcttc cgattttttt cggagaatgg
     4921 caattcgagt ttcgacatca ggtggttgaa gatcggcaat cagtccccac tcaaagcgcg
     4981 accgaagccg ttcttccatt gaaggcattt ctttgggaaa acggtcactc gacaatacga
     5041 tctgttttcc agcttcatag agagaattaa atgtgtgaaa aaattcctct tgtgtccgtt
     5101 ctttccctgc taaaaattga atatcgtcaa tgagcagcat gtccacattt ctgtatcgcc
     5161 ttctcaattc gatcattttg tcatagcgga tggaattaat gacctcattc gtgaattgct
     5221 ctgtcgtgac ataggcaata cggagatcgc catgatcagc aatataattt cctatagagt
     5281 tgagaagatg cgttttcccc agccctaccc cgccataaat gaataacggg ttatacgctt
     5341 tggcaggtga ttctgcgaca gcaagacaag cggcatgtgc aaattgatta ctcgctccca
     5401 cgacaaagtt tttaaaggta tatttggggt tgggaagagg ttgacgtctc acccgttgaa
     5461 gttgagcggg cacccggttt tctcgaactt ccgttttgag ttgaggcgat ggttctccgt
     5521 ctccatcctg ctctttcatg acaaaaacaa tctctgccgg attcacccct tcgacatgct
     5581 gaaaggcctc tttgagtaaa tctttatagt ttct
//
LOCUS       DATIAL010000058        14881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_226593, whole genome shotgun sequence.
ACCESSION   DATIAL010000058 DATIAL010000000
VERSION     DATIAL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14881
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226593"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1464
                     /locus_tag="VLA60_01910"
     CDS             436..1464
                     /locus_tag="VLA60_01910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HSF08135.1"
                     /translation="MLNNPKVTVFIPAYNREKYIGDAIESILAQTFTNFEILLIDDGS
                     KDRTSEIMRSYQDPRVRIIQNEHNLGIPKTRNKGIEQARGEFIAMLDSDDRAFPTRLE
                     KQVNFLDAHHDYAQVGSWCRMMDEQGRPLKRIKRQPILPEDVDIQLLFRCSLSNRSIM
                     ARTKILQEYGYRNDYPRCQDYDLHVRLAQKYKLANLPECLVYGRIHADQITSQTVELG
                     DEKKRAIIRHQLNVLGVPFTEEDLGPHLTLSRMRKSQFTPDDDYLQWAENWLLKLQEA
                     NLQTHRYSQRAIAQAVSEKWIRTCWAAWNGMGWKALKYYVHSPLRKSLGNTIREHVLT
                     RSAQHLFN"
     gene            1490..2752
                     /locus_tag="VLA60_01915"
     CDS             1490..2752
                     /locus_tag="VLA60_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF08136.1"
                     /translation="MTPNSSDFAPLPSQSTKIEGEPPERKHLAIFLPSLAGGGVARAM
                     VYLSEAFADRNHRVDLILCQVSGPYLDSISPKVRVIGLKGGTKWRGCFHAVSADFGAL
                     WSMLLPILLSSHPPKTIGYLPDLVEYLRREKPDTMLTAKTPANLIALLAARLAGGNTR
                     IVINEQTSLSPIIKTSKKWRWRFLAPLLQRVYPWADEIATVADGVAEDVSSLTGIHRD
                     RITTIYNPVALTQVQEKARIPVDHPWFPADTLPVILGVGRHVPQKDFACLIKAFGRVR
                     SVRPARLVILGEGRIRNELEELAETLGLTQDISMPGFVDNPFAFMARASVFVLSSAWE
                     GCPNVLIEALACGCPIVSTDCPSGPTEILQKGAFGPLVPVGDDKALAEAILGVLDHPL
                     GQEQLRARAREFEVGRIAEKYLRLMSAT"
     gene            3069..4835
                     /locus_tag="VLA60_01920"
     CDS             3069..4835
                     /locus_tag="VLA60_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSF08137.1"
                     /translation="MTTSPFSTSTSLSDDAAKRHWSVIAAIGLSLMISFTGIFDHELI
                     ASDEIRVGEIGREILMSGNDLVPTLGGEAFMEHPPLYYWLISVAFRGFGVSDGVARFP
                     SAIAGFLTLLLAFDLSRRLAGPSSGVMTVLVLSSMWGFFRHAHRSMVDPLLSLFVLLG
                     YWAFVLAMFGEKSRNGHPTRLPFSALVFTIYLAGALAFLTKGPVGVILLGSPLALAII
                     FWGRWDFLRSWAHFPGILILITGCLIWPILLYLYEGEVLFKEFVVHNILYRIFPDPEQ
                     YTGGHVKPFWYYFPKLFNQIGPWLLTLPAAYYWAFREKMSQQGLNYSGLRFISIVFPL
                     GLLLLSLPGTKREVYLMPLLAPLAVFIGAWAASLAIQATPSRISRMTANLFGTVSAIL
                     PALLALIIRLFALLLAPLGFLGPRTDRKFALASLPETLQHWSAVVFSFANSWRTRARA
                     IPVFAGLMFTIMLMINLFAWPALGSGRFLRPITVDLAAMDALPPRLVGYQLHEEMRGA
                     LPFYTGLIAQNFQSPEEVSRYVQENPMGFLLLGKYPPSPLSDDLADMLREIKVWEMAE
                     GRYVLYDFLPEPRQPIGVAQPQ"
     gene            complement(4868..5818)
                     /locus_tag="VLA60_01925"
     CDS             complement(4868..5818)
                     /locus_tag="VLA60_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08138.1"
                     /translation="MRLALKVDVDTLRGTVEGVPNLVRVMGRHGVNATFLFSLGPDHT
                     GRALCRIFRPGFLKKVMRTSVGANYGLKTLCYGTLLPGPDIGRKTAPIMREVRDAGFD
                     VGIHCYDHIRWQDYVATKGERWTRHEMTMAAERFMEIFGVPARTHGAAGWQMNEYAFR
                     MEEELKFAYCSDTRGTHPFLPLVAGKPLACPQLPTTLPTLDELIGVDGITEENVTGEI
                     LRRSEQSGPCGHVYTLHAELEGMRFMPVFEALLSGWSERGCQINSMEELSRSLDVSSL
                     PVHRVISNEIEGRTGMLALQGEEVRFPRLRSAGPRKPQGM"
     gene            complement(5825..6871)
                     /locus_tag="VLA60_01930"
     CDS             complement(5825..6871)
                     /locus_tag="VLA60_01930"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HSF08139.1"
                     /translation="MMKKVLILGVNGFIGHHLSKRILETTDWELFGMDMQTDRLADLL
                     PNSKFHFFEGDITINKEWVEYHIRKCDVVLPLVAIATPATYVKEPLRVFSLDFEANLA
                     IVRQCVQHKKRIIFPSTSEVYGMCEDKEFDPETSNLVLGPIHKQRWIYSCSKQMLDRV
                     IWAYGVECSLDFTLIRPFNWIGPGLDSLYTAKEGSSRVMTQFLGHIVRGENISLVDGG
                     RQRRTFTYVDDGVDALMAIIENPNQIASGQIYNIGNPANQYSVRELGEMMVTLALEYP
                     EYAENASKVQFIDVSSNTYYGVGYQDVQHRVPAILNTWRDLNWSPRTTMDQMLRLIFN
                     SYRSEIVQAMALLE"
     gene            complement(6868..7800)
                     /locus_tag="VLA60_01935"
     CDS             complement(6868..7800)
                     /locus_tag="VLA60_01935"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HSF08140.1"
                     /translation="MTRAVVFAYHNVGVRCLSVLLAGHVEVPLVVTHDDSADENIWFD
                     SVAALAQEHSIPFLTPDDPNRPDVIERIRNLAPEFIFSFYYRRMLGAELLACASRGAL
                     NMHGSLLPKYRGRAPVNWAVLKGETQTGASLHYMETKPDAGALVDQQAVPILPDDIAL
                     DVFNKVTWAAELVLYRSLPLLITGQASGQPLNLKEGSYFGGRRPEDGVIDWRESAETV
                     HNLIRSVAPPYPGAFCLLDSLPMRILRSHLDREPARHPDRAPCFYWEVGRCYADCTDG
                     RRLHIGKFEVEGQNMTPEAFHTRYGEQIKDLGQP"
     gene            complement(7797..8756)
                     /locus_tag="VLA60_01940"
     CDS             complement(7797..8756)
                     /locus_tag="VLA60_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF08141.1"
                     /translation="MVEPELTVIIPVYNEEAILPALFSSLYTALDTLGRSYECLFIDD
                     GSTDRSAALLREQFSKRPAHTRIILFNGNFGQHAAILAGFDYMHGLRAVTIDADLQNP
                     PEEIAKLVRKMDEGYDYVGSIRIKRKDSWWRRVASRFINKLREGSTKIRMTDQGCMLR
                     AYDRSIVQAIKRSQEINTFIPALAYLYARNPTEIEVAHSERQAGESKYSLYKLIRINF
                     DLLTGFSLAPLQIFSTVGMVIAMLSGLLVAILLLRRLFVGPEVEGVFTLFGILFFLIG
                     VVLFGIGMLGEYVGRIYQQVQNRPRYIVHATLEDPAPGEAMRK"
     gene            complement(9283..10410)
                     /locus_tag="VLA60_01945"
     CDS             complement(9283..10410)
                     /locus_tag="VLA60_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HSF08142.1"
                     /translation="MESTLPFTRPSLDEETVQAVSEVLHSGWITSGPKVLAFEEALST
                     YLGGNRMVRVLNSGTSALELALALCRIGPGDEVIVPAISFACSANVILRAGARPVFVD
                     VDLTSRNLKLDQVATVITKRTKAIMPVHFAGLPVDMDALYRLAGQHHLRVIEDAAHAI
                     GSGWGGRKIGGFGDLVCFSFHPNKNITTIEGGALSVGDPELVPELERLRFHGIARDAL
                     GSIDVVAAGGKYNLSDVSACIGLHQLRHLDEFVARRAELVGQYFALLGDRSGLILPAR
                     GDEGHSWNMFTVLIPFADLGIERSAFQRAMAARGISIGIHYPAIPGFSLYQSFGYRET
                     DFPNAQRIGRETVTLPLFPAMTHADVKRVCSVLVDVLDSRS"
     gene            complement(10412..10591)
                     /locus_tag="VLA60_01950"
     CDS             complement(10412..10591)
                     /locus_tag="VLA60_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08143.1"
                     /translation="MLWRLALSRVEARIAYAMRSLGCIVNALAVSTLFGEQLTPTKLV
                     VNRIICLGVFVLARN"
     gene            complement(10849..12198)
                     /locus_tag="VLA60_01955"
     CDS             complement(10849..12198)
                     /locus_tag="VLA60_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08144.1"
                     /translation="MRNLFRTASFRLTIFTSGLFLVFVIVTFAIYYWIAVSYVYQRID
                     EEMVWKIQTLTQEYQSGSFDPSDPDLVEPGTYLLLQDASGRIIVKNLPKDWDVQRWLS
                     QLLPEPIPMNNASKTFRAKAVSLPDGSRMVVATELNDIHVVNEFFGEVFVWILVSIVI
                     LSSGCGVLVSTLTLRRVGEINLLARDIMNGHLDRRIPLRGTNDEFDRLSMNLNLMLDQ
                     IKKLMEQVRQVSIDMAHDLRTPLTRLRQRLEGARRQARCMEDYENAIDKALIQNDEIL
                     ETFSGLLRIAYVGSGVDRQRFENVSLTDILETIRETYEPIADERKQSLFSTIESGLIV
                     RADKKLLTQMVVNVLENAIRHSPAETQISIAASHTTGGVMVAIADTGPGVPEAEQRRI
                     FEPFYRMEASRSTSGSGLGLSLVAAIADLHGITINLSDNHPGLRVTLIFPDIPTRKL"
     gene            complement(12191..12868)
                     /locus_tag="VLA60_01960"
     CDS             complement(12191..12868)
                     /locus_tag="VLA60_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF08145.1"
                     /translation="MKILVIEDDQETALYIANGLTEEGYVVDIRADGQEGLMQALDES
                     YEVLIVDRMLPGLDGLSLVRKLRTEKVKTPVLFLSALGEIEQRVEGLEVGGDDYLSKP
                     FAFLELKARIEALRRRSPLQTVDSRLRIGDLEMDLLTRKVRRGEIPIDLQPREFRLLE
                     VLMRYAGRVVTRTMLLEQVWEFYFDPRTSVVETHISRLRTKIDKGFTHHLIHTVRGVG
                     YRMDPDA"
     gene            complement(13702..14076)
                     /locus_tag="VLA60_01965"
     CDS             complement(13702..14076)
                     /locus_tag="VLA60_01965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HSF08146.1"
                     /translation="MMNPTSFSLIIFGVLLNAAAQLLLKAGVGSVGVIALDFGSIFSA
                     GSRLGMHPFILGGLTCYVVSVMIWILALSRVDVSVAYPMLSLGYIVNALAASALFGEQ
                     LTPTKLVGIGIICFGVFVLARN"
     gene            complement(14517..>14881)
                     /locus_tag="VLA60_01970"
     CDS             complement(14517..>14881)
                     /locus_tag="VLA60_01970"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HSF08147.1"
                     /translation="PIHIHVHGLRSASGTVKAVLYGPDPKSFLIKGARADKEREPAQK
                     GSMTLCLAAPMEGKYAVVVYHDENDNHKFDRNLIGLPTEGFGVSKNPSLFLAAPNFEE
                     SSFEVNGAVTHVDVDMKY"
BASE COUNT         3729 a         4022 c         3627 g         3503 t
ORIGIN      
        1 aatcacaaaa aaccaatcgc ttaaattgct gcccattgat tgtcacaaaa tgtaggcttt
       61 tgtccttttg caagcgaact ccaaaaagcc gagataaaaa atatcgggct cgcttcaaga
      121 aaaagggcga tcgttgtttg acctgtaatt gaaaagacat cgtgttgaag agtgtctggg
      181 ttaaaccggt ggtgtcaaaa aaccaaaaat ttggaaaaca ggggatacat cggatatgat
      241 ttgagacccc tagagaagaa tgcgttacta ccgcctgcct gagaaactcc gattaccgaa
      301 tcggcacgtg aactttcagg agatgtgtta tgaacttttt ttaaaaaaga gtcaatatct
      361 tatctaattt tagctccaat agcgcatgaa ttgagaactc ctccggtata actgaaacat
      421 tccggtcaga cgacgatgct taacaaccca aaagttaccg tatttatccc ggcttataac
      481 cgcgaaaaat atattggaga cgccattgaa agtattttgg cacaaacgtt taccaatttc
      541 gaaatactcc tgatagatga tggttcgaaa gaccgtacca gtgaaatcat gcgttcatat
      601 caggatcctc gtgtgcgcat catccagaat gaacacaatc tcggaattcc caaaacccgc
      661 aataaaggca ttgaacaggc tcgtggtgaa tttatcgcca tgttagatag cgacgatcgg
      721 gcgtttccaa cacgactgga gaaacaggtc aactttctgg atgcccatca tgattatgcc
      781 caagtaggaa gctggtgccg catgatggac gaacaaggtc ggccattaaa acggatcaag
      841 cgccagccca ttttacctga ggacgtcgac attcaacttt tgtttcgatg ttccctgagt
      901 aaccgatcga taatggctcg gactaaaatt ttgcaggaat atgggtatcg aaatgactac
      961 ccacgctgtc aggattatga cctccacgtg agactggccc aaaaatataa actcgccaac
     1021 ctccctgagt gcctggttta cggccgtata cacgccgatc aaattaccag tcaaacagtg
     1081 gagctgggtg atgaaaaaaa gcgggcaatc atccgtcatc aactcaatgt cttgggggta
     1141 cccttcaccg aggaggatct cggtccgcat ttgacgttgt ctcgcatgcg aaaatcccaa
     1201 ttcacgccgg acgatgatta ccttcaatgg gcggaaaatt ggttattgaa acttcaagaa
     1261 gccaatttgc agacacaccg gtattcgcag cgcgcaattg cacaagcggt cagcgaaaag
     1321 tggatacgga cctgttgggc ggcctggaac ggcatggggt ggaaagccct gaaatattat
     1381 gttcactccc ccctgcgcaa aagcctcggc aataccatac gagaacacgt cctcacccga
     1441 tcagcccagc atctattcaa ttaagtctca aacaaccttt aatcctatga tgacgccgaa
     1501 ttcctcggat ttcgccccac tcccgagtca atcgacaaag atagaaggtg aaccaccaga
     1561 gcgtaaacat cttgcgatat ttctaccatc tctagccggg ggcggcgtcg caagggctat
     1621 ggtctatctg tcagaggcct ttgccgatcg caaccacaga gtggacctga tcttatgtca
     1681 ggtctcaggg ccctatctgg acagcatttc ccccaaggtt cgggtcatcg ggctcaaggg
     1741 gggaacaaaa tggcggggat gcttccatgc cgtgtcagct gattttgggg ctttatggtc
     1801 aatgctcctc ccgatcctgc tatcgtccca tcctcccaaa actatcggat atctcccgga
     1861 tctggttgaa tatttacggc gtgagaaacc agatacgatg ctcacggcga aaacccctgc
     1921 gaacctcata gccttgttag ccgcacgcct tgcaggtggc aacactcgca ttgtgatcaa
     1981 cgaacaaacc agcttgtctc ccatcatcaa gacatccaaa aagtggcgtt ggcgtttcct
     2041 tgcacctctc ttacaacgcg tgtacccatg ggcagatgaa atcgcgaccg tggcggatgg
     2101 cgtcgcggaa gatgtgtcct cgttaacggg aatccatcgt gatcgtatta cgacgattta
     2161 caatccggtg gccctcaccc aggtacaaga aaaagcccgc atcccggttg accatccgtg
     2221 gtttccggca gatactctgc cggtcatcct aggtgtaggg agacatgtgc cacaaaagga
     2281 cttcgcgtgt ctcataaagg cctttggacg agttcgttct gtgcgtccag cccggctggt
     2341 gatcctcggg gagggaagaa tacggaatga gcttgaagag ctggctgaaa ccttgggcct
     2401 cacccaagat atttccatgc caggatttgt cgacaatccc ttcgccttta tggccagagc
     2461 ttcggtattt gtgctctcgt cagcatggga aggttgtccg aatgtgctga tcgaagcgtt
     2521 agcatgtgga tgtccgattg tcagcacgga ctgcccgagc ggtcctaccg agattctcca
     2581 aaaaggggcc tttggaccat tggtaccggt cggtgatgac aaagcactgg ccgaagctat
     2641 tctcggagtc cttgaccatc cacttggcca ggaacagttg cgtgcccgcg ctagggagtt
     2701 cgaggtgggc aggatcgcgg aaaagtatct ccggcttatg tcggcgacct aatttctctt
     2761 cacatgactc tgttaagaga ggcgtgacat tgatagaacc acatgagaac cggttttcaa
     2821 tagggaccac actcgcgacg gtcttggatt tctcccttta taaattccgg gttatgcaga
     2881 ataaagcttc tcttcatatc caaccccgct cctcatagca aatcaatatg gttgccggaa
     2941 acctatctca cgacacttct atccggtgag agcgcatcat catggccagg aagtctaatc
     3001 cccttggagc ttccagccaa taggcaaaat agccagctgg cagacaatca aatgaggttg
     3061 aagatcctgt gaccacatct cccttctcaa ccagcacatc tctctccgat gacgccgcaa
     3121 aacggcattg gagcgtcatc gccgcaattg ggctgagcct gatgatctcg tttacaggta
     3181 ttttcgatca tgagctgatt gcctccgacg agattcgagt cggcgagatt gggcgggaaa
     3241 tactcatgag tggcaacgac cttgttccca ccttaggcgg ggaagctttt atggagcacc
     3301 cccctttata ttactggttg atttcggtcg cattccgtgg ttttggcgta tccgacggcg
     3361 tcgcacgatt tcccagtgcc atcgcggggt ttttgaccct cttacttgcc ttcgacctca
     3421 gccggcgact tgcgggcccg agtagcggcg tgatgacagt cctggtcttg agctccatgt
     3481 ggggattctt tcggcatgcg catcgttcca tggtggaccc gctgctctcg ttgttcgtcc
     3541 tgcttggata ttgggcgttt gtgcttgcca tgtttgggga aaaatcacgg aatggccatc
     3601 caacccgttt acccttctcg gccctggtct tcaccattta tcttgcaggg gccctggcat
     3661 tcctgaccaa aggacctgtg ggcgtcatct tgctcggaag cccattggct ctggcaatca
     3721 tattttgggg tcgctgggac tttttgcgtt cctgggcaca ctttcccggc attctcattc
     3781 tgatcacagg atgtctcatt tggcccatcc tgctttacct ctacgaaggg gaagtcctgt
     3841 ttaaagaatt tgttgtgcac aacatcttgt atcgcatttt ccccgatcct gagcagtata
     3901 caggcggcca cgtgaaacct ttctggtact actttcccaa gctgttcaat caaataggac
     3961 catggctcct tacgcttcct gcagcctatt actgggcatt tcgggagaaa atgtcccagc
     4021 agggtctgaa ctattcaggt ctgcgcttca tttccatcgt tttccctcta ggactcttgt
     4081 tgctgagcct tcccggtacc aaacgggagg tatacctgat gccgcttctt gcacctcttg
     4141 ccgtctttat tggggcctgg gcggcctcgc tggcaataca agctacacct tcacggatct
     4201 cacggatgac ggccaatctt ttcggtacag tttcggccat ccttcctgct ctgctagcac
     4261 tcatcattcg cctgtttgct cttttgttgg cccctcttgg tttccttggc ccccgtactg
     4321 accgcaagtt tgccttggcc agtctccctg aaactttgca acactggtcg gctgtcgtat
     4381 tctctttcgc caattcatgg cgcacaaggg cgcgggccat ccccgtcttt gccggcttga
     4441 tgtttaccat catgctgatg atcaatttgt ttgcttggcc ggctctaggg agtggaagat
     4501 tcttgcgtcc tattaccgtc gatcttgcag ccatggatgc cctaccccca cgactggtgg
     4561 gctaccagct acatgaagag atgcggggag cattgccatt ttacacaggt cttattgcgc
     4621 aaaatttcca atcaccggaa gaagtgagtc gttatgtgca ggagaatccg atgggatttt
     4681 tactactcgg caagtacccc ccctcgcccc tttccgatga tcttgctgac atgctgagag
     4741 agattaaggt ttgggagatg gcggagggtc gctacgtgct ctatgatttt ttgccggaac
     4801 cccgccaacc tattggagtt gcccagccgc agtaaggtat ttaagggtac cgggggtgaa
     4861 ggatttttta catgccctgt ggtttccgtg gtcccgcaga tctcaaccgt gggaagcgga
     4921 cttcctctcc ttggagcgct aacatcccgg tgcgtccttc tatctcatta gaaatgacgc
     4981 gatgcacagg taacgaggag acatccaggg aacgagagag ctcttccatg gaattgatct
     5041 gacacccgcg ttcgctccaa ccagaaagca gcgcttcgaa caccggcata aatcgcatac
     5101 cctccaactc tgcatgcagc gtgtatacat gtccgcaggg accggattgt tcactccgtc
     5161 tcagaatttc acccgtcaca ttctcttcgg taattccatc gactccgatc agctcatcga
     5221 gtgtgggcaa tgtcgtcggt aactgcgggc acgcgagcgg tttaccggct acaagaggaa
     5281 gaaacgggtg cgtacccctg gtatcgctgc aataggcaaa tttcaactct tcctccatgc
     5341 gaaaagcata ttcgttcatc tgccagccgg ccgcaccgtg cgtgcgcgca ggcacgccaa
     5401 aaatctccat gaaccgttcg gccgccatgg tcatctcatg ccgggtccac ctttctccct
     5461 tggttgcaac gtagtcctgc cagcgaatgt gatcatagca atgaataccg acgtcaaagc
     5521 ctgcgtcgcg cacctcacgc ataatagggg ccgtctttcg cccaatgtcc ggcccaggaa
     5581 gcagcgtgcc ataacacaat gtcttaagcc cgtaattggc cccgactgat gtgcgcatca
     5641 cttttttgag aaatccgggc cgaaagatcc ggcacagggc acggcctgta tgatcaggcc
     5701 caaggctgaa cagaaaagtc gcattgacgc catgacggcc catcacacgc accaggttag
     5761 gcacaccttc aaccgtgcct cgaagagtgt caacatcgac tttgagggcg aggcgcatgg
     5821 ggcgttattc caacaacgcc atggcctgca caatttcgct gcgataggaa ttaaagatga
     5881 ggcgcaacat ctgatccatc gtcgtcctgg gggaccaatt caggtcacgc catgtattaa
     5941 gaatggcagg cacgcggtgt tgaacgtcct ggtagcccac cccgtagtac gtatttgagc
     6001 tgacatctat gaactgtacc tttgaagcat tttcggcata ctccggatat tctaaggcga
     6061 gggtcaccat catctctccg agctcacgca cggagtactg attggccgga ttaccgatgt
     6121 tatagatttg accgctggcg atctggttgg ggttttcgat aatcgccatc agcgcatcaa
     6181 cgccgtcgtc cacataggtg aaggtcctcc tttgcctgcc cccatccacg agagaaatat
     6241 tctcaccccg cacgatgtgt cccaaaaatt gcgtcatcac ccgcgaactc ccctctttgg
     6301 ctgtatacag gctatcaagt cctggaccaa tccagttgaa gggacggatc agcgtgaaat
     6361 ccaaggaaca ttccacaccg taggcccaga tgacccggtc cagcatttgt ttcgagcacg
     6421 agtagatcca gcgctgtttg tggattggac caagcaccag attcgaagtc tcggggtcaa
     6481 attccttatc ctcgcacatc ccatagactt cggaggttga gggaaagata atccgctttt
     6541 tgtgttggac acactgccga acaatcgcga gattggcttc aaaatccagc gaaaacactc
     6601 tgagcggttc cttcacatag gtcgccggtg tggcaatggc gaccaatgga agcaccacgt
     6661 cgcatttgcg tatgtggtat tccacccatt ccttattgat cgtgatatcc ccctcaaaaa
     6721 aatgaaattt tgaatttggc agcaggtcag ccaagcgatc ggtttgcata tccattccaa
     6781 agagttccca atcggtggtc tccagaatgc gcttactcag atgatgcccg ataaatccat
     6841 tgacgccgag aattaagact tttttcatca tggttgaccc aaatccttta tctgttcccc
     6901 gtaacgggta tgaaaggcct caggggtcat gttttgaccc tccacctcaa acttgccgat
     6961 gtgaagacga cgtccatcgg tgcaatcagc gtagcaacgc cctacctccc agtagaagca
     7021 gggcgcacgg tcaggatgcc gtgccggttc ccgatcaaga tgacttctca ggatccgcat
     7081 gggtaaagaa tccagaagac agaaggctcc tgggtaggga ggggcaaccg atctgatcaa
     7141 gttatggacc gtctctgcac tttcccgcca atcgataacc ccatcctccg gccgacgtcc
     7201 accaaaatag gatccctcct taaggttcag cggctgaccc gacgcttgcc ccgtgatcaa
     7261 caacggtaag ctgcggtaca gaacaagctc agcggcccag gtcaccttat tgaatacgtc
     7321 cagcgcaatg tcgtccggaa gaatgggaac ggcttgctgg tcaaccagcg cgccggcatc
     7381 cggcttggtt tccatataat ggagactcgc gcctgtttgc gtttcgcctt tgagcaccgc
     7441 ccagttcacc ggggcgcgtc cacgatattt tggcagcaag gacccgtgca tattcaaggc
     7501 accacgtgac gcacatgcca gcaactcggc gcccagcatt cgccggtaat agaaggaaaa
     7561 gatgaattcg ggagcaaggt tgcgtatccg ttcaatcaca tccggccggt taggatcatc
     7621 cggggtcagg aagggaattg aatgctcttg agccagtgcc gccacgctat cgaaccagat
     7681 gttttcgtcg gcggaatcgt catgagtgac caccaacggg acctcgacgt gccccgccag
     7741 gagaacggac aagcagcgca cacccacatt atgataggca aagacaaccg ctctggtcat
     7801 tttctcatcg cttcacctgg agcaggatcc tccagtgtgg catgcacaat atagcgagga
     7861 cggttttgca cctgttgata gatgcgcccg acgtactccc ctaacatccc gatgccgaac
     7921 agcacgaccc cgataagaaa gaacagaatc ccaaagaggg taaatacccc ttcaacttct
     7981 gggccaacga acagccgccg cagcaggagg atcgccacaa gcaaaccgga caacatggcg
     8041 atcaccatgc cgaccgttga aaatatctgc agcggagcca gcgaaaatcc tgttaacagg
     8101 tcgaagttga tacgaataag cttgtacaac gaatacttcg actcgcccgc ttgacgttcg
     8161 ctatgggcga cttcgatttc cgtggggttc ctggcataca ggtaggcaag ggcaggaata
     8221 aacgtgttga tctcctgaga ccgtttaata gcttgaacga tggaacgatc ataggctcgt
     8281 aacatgcagc cttgatctgt catccgaatc ttggtcgatc cttcgcgcag tttattgatg
     8341 aagcgcgatg cgacccgacg ccaccaacta tccttgcgct tgatgcgaat ggatccgaca
     8401 taatcgtagc cttcatccat cttacgaaca agcttggcaa tctcttccgg cggattttgc
     8461 agatcggcgt cgatcgtgac cgcccgcaga ccatgcatat aatcaaaccc ggctagaatc
     8521 gccgcatgtt gaccgaagtt gccgttgaac aaaatgatac gcgtatgggc cggacgtttg
     8581 ctgaattgct cacgcaacaa ggccgcagag cgatccgtgc tgccatcgtc gatgaataga
     8641 cattcataac ttcgtcccaa cgtatccaat gcggtataca gactggaaaa caaggccggc
     8701 aaaatggcct cttcgttata caccggaatg atcacggtga gttcaggttc aaccatgtcg
     8761 gcggcagcag gaaaaaatag attgcaacat aaacggcggg atatcctcac ttatacaaaa
     8821 tgcccaaact ttgcgaagtg gtaatctagg gacccctgtc atcgaattcc tcccaattca
     8881 cctgtgacag ccgacaaagc aacgaggctc atcaatgata aaaattgaac aggccgctca
     8941 tagcgaagtc gcacacttct cttgctaatg ctctttacaa taccctgccg ggctcattct
     9001 ttttcctgtt gacgtaattc catcatccat agcataaacc atgcctccca aattcttgta
     9061 taggatgtct gccataattc ggaagagttg atttactcac agtccagtgg aaagtcacag
     9121 ggtcagatgt tgtcggcaat gcaacgggct gttcacacaa aactgctctc tcacctcata
     9181 agaccggcgt tccccatgac agaaaatata ggggaatgag gtgagaatgc tccatcgtgc
     9241 aagcgtgatg tgtggcttat atgagcgatt tgacaacctt ctttatgatc ttgaatccaa
     9301 cacatcaaca agcacggaac acactcgttt cacatctgca tgagtcatgg cagggaacaa
     9361 tggaagggtc acggtctcgc gcccgatgcg ctgggcgttc gggaaatcgg tctcgcggta
     9421 cccaaagctt tgatacagac tgaatcctgg aatggcgggg tagtgaatac ctatgctgat
     9481 accccgggcc gccatcgcac gctgaaaagc actccgctct atgccgaggt ctgcaaacgg
     9541 gatcaagacg gtgaacatgt tccaactatg gccttcatcc ccgcgtgccg ggagtatgag
     9601 accggagcga tcgccgagca gggcaaaata ttggccgacc agctccgccc gcctggccac
     9661 aaattcatcc aggtgccgga gttggtgtaa gccgatacag gcagatacgt ctgataaatt
     9721 gtattttcca ccggcggcaa ccacatcaat ggagcctaga gcatcgcgtg cgatgccgtg
     9781 aaatcgaagt ctttcaagct ccgggacaag ctcaggatca ccgacagaga gcgcaccacc
     9841 ctctatcgtg gtgatgtttt tgttgggatg aaagctaaaa cacacgaggt caccaaaccc
     9901 gccaatcttg cgacctcccc acccgctccc aatagcgtga gccgcatcct cgatgacacg
     9961 taggtgatgc tgaccggcca aacgatagag tgcatccata tcaaccggca acccggcgaa
    10021 atgcaccggc atgatcgctt tggtgcgttt cgtgatcacc gtcgcaacct gatcgagttt
    10081 gagatttcga ctggtcaagt cgacatccac aaacaccggt cgtgcgccgg cacgcagaat
    10141 gacattcgca ctgcaggcaa aacttatcgc gggcacgatg acctcatcac caggcccgat
    10201 gcgacaaagg gcaagggcca attccagtgc gctggtgccg gaattcaata cccgtaccat
    10261 ccggttgcca ccgaggtacg tactaagcgc ctcctcaaaa gccagcacct tcgggccgct
    10321 ggtgatccag ccggagtgaa gcacctcgct aaccgcctgg acggtttcct catcaaggga
    10381 gggacgggtg aacggtagtg tcgattccat ttcaattcct tgcgagcacg aacacaccca
    10441 aacaaatgat acgatttacg acgagctttg tcggcgtcaa ctgctcacca aacagggtag
    10501 agactgcgag agcattgacg atacaaccca acgaccgcat cgcataggct atgcgcgctt
    10561 ccacccggga aagagcaagt ctccataaca tgacactgcg gaatacccgg tgaagctgcc
    10621 tagaatgaaa agatccaccc aggcgtgaat ccgccaacaa aagttgccaa aatccaacgc
    10681 gatgcatccg acgcgactag cttccgcgtt caaaggaagc tgggcggcag cattaagcca
    10741 tacgccacaa acgttgcact cctatccatt aaccgataga aataaagtat agccaatagc
    10801 tcaggattat taccgcaact atttcaattt attaccaaat ggtatgagtt acagcttacg
    10861 ggtagggata tccgggaaga tcagagtcac gcgcaggccg gggtggttat cggaaagatt
    10921 aatggtgatg ccatgaaggt ccgcaatcgc agcaactaag ctaagtccga gtccgctacc
    10981 cgacgtgctt cggctggctt ccattcgata aaagggttca aaaatcctgc gctgttccgc
    11041 ttcggggact ccaggccctg tgtctgcgat tgccaccatt acgccgcccg ttgtgtgaga
    11101 agcggcaatt gaaatttggg tttccgccgg ggaatggcgg atagcattct ccaggacgtt
    11161 aacgaccatt tgggtgagca attttttatc ggcacggact atcagtccgg actcgatggt
    11221 actgaacagg gattgcttac gctcatcggc aatcggctca taggtctccc ttatcgtttc
    11281 gaggatgtcg gtcagtgaga cattctcgaa cctctgtcga tcaaccccgg accccacata
    11341 cgcgattctc aaaagaccgc taaatgtttc taaaatctcg tcattttgga taagggcttt
    11401 gtcgatggca ttttcataat cttccataca ccttgcctgc ctgcgagctc cttccaatct
    11461 ttgtcgcagg cgtgtcagcg gcgttctcaa atcgtgagcc atatctatcg agacctgacg
    11521 tacctgctcc atcagttttt taatctgatc caacatgaga ttaaggttca tgctcaatcg
    11581 atcaaattca tcgttcgtcc ccctgagggg aatccgacga tccaggtgcc cgttcatgat
    11641 gtctctcgct aggagattga tttctcccac acgccgaagg gttagggtgc tgaccaaaac
    11701 tccacatcca ctgctcaata tgacaatcga caccaatatc catacaaaga cctcaccaaa
    11761 aaactcatta accacatgaa tgtcatttaa ttcagtagcc acgaccatgc gtgaaccgtc
    11821 cggtaatgaa acagctttcg cacggaaggt cttgctggca ttgttcattg gaatgggctc
    11881 tggcaggagt tgggataacc accgttgaac atcccaatct ttgggaaggt tcttcacaat
    11941 gattcgacca ctcgcgtctt gcaacagaag gtaggttccc ggctccacca gatcgggatc
    12001 agaaggatcg aacgatcccg actgatactc ctgggttaac gtctggatct tccagaccat
    12061 ctcctcgtct atacgctggt agacgtagct caccgctatc caataataaa tcgcaaatgt
    12121 cacgatcacg aagaccagga agaggccgga cgtgaaaatg gttaatcgga aacttgcggt
    12181 acgaaagagg ttacgcatcc gggtccatac ggtaccccac tccacgcacg gtatgaatca
    12241 aatggtgagt gaagcccttg tcgatctttg ttctcaagcg gctgatatgg gtctcgacca
    12301 cgctggtgcg aggatcaaaa taaaactccc acacctgctc aagaagcatc gtccgggtga
    12361 cgacacgacc tgcataccgc atcagaactt cgaggagacg gaattcacga ggctgaagat
    12421 caatcgggat ctcccctcgt ctcaccttcc gtgtgaggag atccatttca agatcaccga
    12481 ttcgcaatcg cgaatctacc gtttgaaggg gagaacgtcg acggagagcc tcaatccgag
    12541 ccttcaattc caaaaaggca aagggtttag atagatagtc gtctcctccg acttcgagac
    12601 cctcgacccg ctgttcaatc tcaccgaggg cactgagaaa gagcacggga gtcttcacct
    12661 tttcggtgcg aagttttctt acaagactga gcccgtcgag accgggtaac atccggtcaa
    12721 cgatcagcac ctcataggat tcatcgaggg cttgcatcaa tccctcttgg ccatcggctc
    12781 taatgtcgac aacatatccc tcttcggtga gaccgttggc gatgtataag gctgtttctt
    12841 ggtcgtcttc aattacgaga attttcatgt cactcacatt tcttatggca gaacaccttg
    12901 cctgatttta tctaccccgg atggcaatct tgggaccgca tacggaaagc ataccccaat
    12961 taagccaaaa ttttcattcg gaaggcccag aatcaaccgg aatattgcat tagccgatct
    13021 gcgactgatg tggcacgcct ggttgcgacc aagccatagg aggatatcgg tgggtgggtt
    13081 gcttactttt caacaaggcc gtggcaccac tgccccttcg gcattccatc aagttcgtcc
    13141 aagccgactc ccggttttgc gccaaggcca tcttgcccca ctctgaagtc cgcgctctcc
    13201 ctccaaggtg gcgtaggcgg cttaccggga gtgcgcctct ggcgaccggg agtgacggct
    13261 gaggcaaaaa agccgggcgg cctcagacac aggtaaaatt atgaaaaata ttcaggggac
    13321 tttttatgat cattccctcg ctccaccggt caaattctgg cgactgtaca ggcaccatgc
    13381 agattcgaag aatcggatcc tggaaatcaa aaaccatctc aggctcccag gcttctcccg
    13441 gaactccttt tggtcaaaga cgccattttt cttggggttg ttggtctata ttacttatga
    13501 gggaattccg gcatgccttg cgacgagcca ccaaggatgt gcacctgtag accatgcggt
    13561 taacccggag atagctattg cgcaccttca caaaggcaag cacctgcaga ccaaggaggt
    13621 ccaatcggaa aaagttcctc gttgaccgcc tagacgtttt ccttatcgag ggagggacag
    13681 aatggcagtg tcgggtcaga ttcaattcct ggcgagcacg aacacaccga aacaaatgat
    13741 accgatgccg acgagctttg tcggcgtcaa ctgctcgcca aacagggccg aagccgcgag
    13801 agcattgacg atgtagccca atgacaacat cgggtaggcc acacttacgt ccactcggga
    13861 cagagcaagg atccaaatca tgacactgac gacataacag gtgaggccgc cgagaatgaa
    13921 gggatgcatt cccaggcgtg aacccgccga gaaaatgctg ccaaaatcca acgcaatgac
    13981 tccaacgcta cccactcccg ctttcaaaag aagctgggcg gcggcgttaa gcaatactcc
    14041 aaagataatt aaagaaaaac ttgttggatt catcatgcta tgagaccatc gccttatcca
    14101 tgttgcaaat atttcccacc tgcatccacc ttctaaactc taaatttttt agttgctttt
    14161 tcgtggaaaa atgagggcga ttccagaata aagacgcgaa gcgaacctag ggggaatgcg
    14221 gagggaaaat cagccttcac ccccctaagc ccctttttca cacgaggaac ttcatcaggg
    14281 aaactccgct ccaggttagg aggaataata ccttcaaagg ctgtggactt atagcagact
    14341 tttatctgga gaataaagga gaagggccgc aaggacttat gcggtatcca cgatagttac
    14401 ccttttaaca tttcacgggt ttcctggtgt acatccggtt caccggtata ttcctatggc
    14461 aacaatccaa gtggctgtat gcgtttacat gcccaggcaa cggtgatgat atggcactaa
    14521 tatttcatgt caacatctac atgggtcact gccccattca cctcgaacga ggattcttca
    14581 aaattcggag cggctaaaaa taacgaggga tttttcgaca cgccaaaccc ttcggtaggc
    14641 aagccaatca gattacggtc aaatttatga ttgtcgtttt catcatgata gacaaccacg
    14701 gcatattttc cttccatcgg cgcagccaaa cacagggtca tagaaccttt ttgagcaggt
    14761 tcccgttctt tatcagccct ggctcccttg attaaaaaac tcttggggtc cggtccgtaa
    14821 agaacagctt tgactgttcc tgaagcacta cggagcccat gcacatgaat gtgaatgggc
    14881 t
//
LOCUS       DATIAL010000059         6957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_871731, whole genome shotgun sequence.
ACCESSION   DATIAL010000059 DATIAL010000000
VERSION     DATIAL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871731"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VLA60_01975"
     CDS             complement(<1..284)
                     /locus_tag="VLA60_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HSF08148.1"
                     /translation="MKKRIAVLPGDGIGPEIMPQAIRVLEAIGQKCGHQFTFTYGQVG
                     GEAIDATGFPLPAETLKLAQSSDAVLLGAVGGPKWEGLEYERRPERALLG"
     gene            complement(435..1982)
                     /locus_tag="VLA60_01980"
     CDS             complement(435..1982)
                     /locus_tag="VLA60_01980"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HSF08149.1"
                     /translation="MTRMIRIFDTTLRDGEQSPGASMNIEEKILVAKQLARLNVDIIE
                     AGFAFSSPGDFEAIHRIAQEVEGPVICSLARAKPEDIDRAWEALKGAPKCRIHTFLSS
                     SDIHLKHQFRMTRDEALKRAVEMVRHARGYVEDVEYSPMDATRSDLTYLCEVVEAVVE
                     AGAGTVNIPDTVGYTTPEEFGKIIRTLRKRVRGMETAIISVHCHNDLGLAVANSMAAI
                     YAGAGQVECTINGIGERAGNASLEEIAMGLRTRNDFYQADTNIRTEEISKASRLVSNI
                     TGMVVQPNKAIVGANAFAHTSGIHQDGLLKDKSTYEIMRPESVGLTQSRLVMGKLSGR
                     HAFREELANLGYTLSDQELQEAFERFKHLADQKKELFEEDLETIVNKAITKVPERYAL
                     KGLHVESGLDRRPSAMVELMMDGRIAKLTGTGDGPVDAVYRTIAALTETKSLLRAYIV
                     KAITGGTDAQGEVSVRVEENSETVTGHGSDTDIILASAQAYLNALNKLADLAERHARA
                     EQKIGLC"
     gene            complement(2046..2816)
                     /gene="pssA"
                     /locus_tag="VLA60_01985"
     CDS             complement(2046..2816)
                     /gene="pssA"
                     /locus_tag="VLA60_01985"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HSF08150.1"
                     /translation="MKQPKKRRVVYLIPNLLTTGNLFSGLASILFVFGDDYGAAAIAI
                     LVAIVFDVLDGTSARLMKSTSDFGLQYDSLADVVAFGVAPGLLMYSWALSAPKMLGAA
                     VMFAFVACGALRLARHNVLATTGGDGKPFTGLPIPGAAGVMATLVIFDQHVAPLGEKV
                     KPILGIILTLVLAFLMVSTFRYRSLKELKTQEHHHFNYLVYAVLILMAVLAYPQAMLF
                     VVFAAYALSGVLEQGWKLVAGMMGKKPGEEIRPDGLHK"
     gene            complement(2813..3451)
                     /locus_tag="VLA60_01990"
     CDS             complement(2813..3451)
                     /locus_tag="VLA60_01990"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HSF08151.1"
                     /translation="MPKKASPLSQGGGLLTLGCWVAGSVWLTCLMGSLTLFTAWFFRN
                     PVRTIPQGKNLIVSPGDGTVVAVEEEFEHRFLKEPSIRISIFLNVFNVHINRMPAAGV
                     LQDVVYAPGNFLVASRPEASAENEQNALMLRRSDGKKILCVQIAGLIARRIVCWVTPG
                     EQVEAGERFGLIRFGSRMDLYLPHDSAIQVKVGEKVKGGSSIVAELLCVEPS"
     gene            complement(3513..4529)
                     /gene="ilvC"
                     /locus_tag="VLA60_01995"
     CDS             complement(3513..4529)
                     /gene="ilvC"
                     /locus_tag="VLA60_01995"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HSF08152.1"
                     /translation="MMKIYYEKDADRQILAKKTVAVVGFGSQGHAHALNMRDSGLQVV
                     VGCREGSSWNKAEAAGLKVMPTADAVKGADVVMLLAPDEAQAAIYNKDIGPNLKQGAY
                     LAFGHGFNIHFGQIQPASHVNVFMVAPKGPGHLVRSEYTKGTGVPCLLAIHQDPGGQT
                     AKVGLAYACAIGGARAGVIETSFREETETDLFGEQAVLCGGLTSLIQAGFETLVEAGY
                     SPEMAYFECLHEVKLIVDLIYQGGIANMRYSISTTAKYGDVTRGPRVVTDETKKVMKE
                     ILGEIQSGRFAREWVLENQANRPVYNALLRRGEEHPIEEVGARLRGMMPWLQKDQLVD
                     KQKN"
     gene            complement(4623..5144)
                     /gene="ilvN"
                     /locus_tag="VLA60_02000"
     CDS             complement(4623..5144)
                     /gene="ilvN"
                     /locus_tag="VLA60_02000"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HSF08153.1"
                     /translation="MEHIISLTVENKFGSLSRVAGLFSGRGFNIESLSVAPTLDPSVS
                     MMTLVTKGDDPIIEQILKQLNKLIDVIKVVDISESEFVERETALIKVHTRPEDRAEAL
                     RIADIFRANVLDSSPTSYTIEVTGDVRKVQAIINLLQPLGIKELVRTGRIAIGREPTR
                     STQTQSRPLAKAN"
     gene            complement(5167..>6957)
                     /gene="ilvB"
                     /locus_tag="VLA60_02005"
     CDS             complement(5167..>6957)
                     /gene="ilvB"
                     /locus_tag="VLA60_02005"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HSF08154.1"
                     /translation="QRVRIMKITGAEIFLEALKREGVKTMFALPGGVVLKIFDVLHQQ
                     KDIRVILTRHEQGAGFMAVGYAKAMGKPGVALITSGPGMTNVITSLADAYMDSVPIVV
                     FSGQVPTALIGNDAFQEADNIGLSRPCTKYNFLVKDVKDLAQTIKEAFYIATTGRPGP
                     VLVDIPKDISLDKADFHYPTSVSIRGYNPTYDGSRWKIKQAADAIMKAKRPILYVGGG
                     VVLSGASPEVKELAELTQIPVDMTLMALGAFPGNHPLSLGMLGMHGTYVANMAMHYSD
                     LVIAVGARFDDRVTGKVSEFCPDAKIIHIDIDPTSIRKNIQVDIPIVGDCKRVLIELN
                     NILRATVNGNQKEQRKPWWDQLNAWRQAHPLRYDQDPDGQIKPQFLIDRLYQLTSDRN
                     PILATDVGQHQMWSAQYFKLQNPQSWLTSGGLGTMGFGLPAAMGAQAAFPDRLVLCVA
                     GDGSIQMNTQELATAVVEQLPVKVFIINNRFHGMVRQWQDLFYEGRYASSYLGTVPDF
                     VKLAEAYGAAGIRIEKVSEMDSGIREALSINGPVVIDVPTYQFENCYPMIPAGGCNHE
                     MLLADPPDLKKPNAMQKIGTQADSDSVITA"
BASE COUNT         1735 a         1988 c         1508 g         1726 t
ORIGIN      
        1 aacccgagta aggcacgctc cggccgacgc tcatactcaa ggccttccca cttcgggcca
       61 cccacagctc ccaagaggac ggcatcactg gattgagcca atttcagggt ctcggccggt
      121 aatggaaagc ccgtcgcgtc aatcgcctcc ccgccaacct gcccataggt aaaggtaaat
      181 tgatgcccac acttctgacc aatggcctcg agaacccgaa tcgcttgtgg catgatttca
      241 ggtccgattc catcacccgg caatacggca atccgtttct tcatgattcc cccaataata
      301 tgatgaacat ctttggtttg tttccggcga caaacacgca ccctaaacgg tctgagagaa
      361 ggcacacacc catggacgaa gaaaatggca ggagatcagt ggctcattga cttatggtga
      421 agggaacgcg ttcttcaaca caacccgatt ttttgttcgg ctctggcatg tcgttccgcc
      481 aaatccgcca atttattgag cgcattaagg taggcctgtg cagaagcaag aataatatcc
      541 gtatcggatc cgtgaccggt aacggtttca ctattttcct cgacgcggac cgacacttct
      601 ccctgggcat cggtaccacc tgtaatcgct ttcacgatat aggcacgaag aagacttttc
      661 gtttcagtca aggctgcaat ggtccggtac accgcatcca ccggcccatc acccgtacct
      721 gtcagtttcg cgattcgccc atccatcatc aattccacca tcgcggacgg acgccgatcc
      781 aagccacttt ccacatgcaa cccctttaag gcatagcgct caggaacttt ggtaatggcc
      841 ttattgacga ttgtctccag atcttcttca aataattcct tcttttgatc cgccagatgt
      901 ttaaagcgtt caaaggcctc ttgcaattct tgatcagaaa gagtgtaccc cagattcgcc
      961 aattcttcac gaaaggcatg ccgaccggac agtttgccca tcaccaaacg actttgggtt
     1021 aatccgacgg attccggtcg cataatctca taggtacttt tgtctttgag caacccgtcc
     1081 tgatgaatac cggacgtatg cgcaaaggca ttggcaccca caatcgcttt attgggctga
     1141 accaccattc cggtgatatt gctgaccaac cgactggctt tggagatttc ctccgtgcgt
     1201 atattggtat cggcctggta aaaatcgtta cgcgtacgga gtcccatcgc gatttcttct
     1261 aaggaagcat tgccggctcg ttccccaatg ccgttgatcg tacattccac ttgacctgct
     1321 cccgcatata tggccgccat ggaatttgcg acagccaagc ccaagtcgtt atggcaatgg
     1381 acggaaataa tggccgtctc catcccccgg actcgctttc ttaatgtccg aataatcttt
     1441 ccaaactcct ctggagtcgt atacccaacc gtatccggaa tgttcaccgt tcccgctcca
     1501 gcttccacta cggcttccac cacttcacag agataggtca aatcggaacg ggtggcatcc
     1561 atgggagaat attccacatc ctcaacataa ccccgggcat ggcgaaccat ctccactgcc
     1621 ctcttaaggg cttcgtccct ggtcatgcga aattgatgtt tcaggtggat atccgaagaa
     1681 gacaaaaacg tatggatacg gcatttgggt gcgcctttga gtgcctccca agcccggtca
     1741 atatcttccg gtttcgccct ggccagactg caaataaccg gaccttccac ttcctgggca
     1801 atacgatgaa tcgcttcaaa atcaccagga gagctaaacg caaaaccggc ttcgataata
     1861 tccacattca atcgggctaa ttgcttcgcc acgaggattt tttcttcaat gttcatgctc
     1921 gcgccaggag actgctctcc atcccgcaag gtggtatcaa aaattcgaat catccgtgtc
     1981 atggctcttt acccccacat ttggtcttca tccaacgccc ccttctcctc tcgatggcgg
     2041 gccattcatt tatggagtcc atccggtcta atttcttccc ccggcttttt ccccatcata
     2101 cctgccacca gcttccaccc ttgctccaat acacccgaaa gcgcataggc tgcaaacacg
     2161 acaaaaagca ttgcctgtgg atacgctaag accgccatga gaatgaggac tgcatacacc
     2221 aaataattaa agtggtggtg ttcctgcgtt ttcaattctt tcagactacg ataacgaaat
     2281 gtactcacca tgagaaacgc cagcaccagg gttaagatga tcccaagaat cggcttcacc
     2341 ttctccccta acggcgcaac atgttgatcg aaaatcacca acgtggccat cacccctgct
     2401 gcaccgggaa taggaagacc ggtaaacggc ttgccgtctc cacctgtcgt cgccaaaacg
     2461 ttatgtctgg ctaatctcag ggcgccacac gcgacaaagg caaacatcac cgccgcgccc
     2521 aacatcttcg gagcactcag cgcccatgaa tacatcagca agcccggagc cactccgaaa
     2581 gccacgacat ccgccaacga atcatattgc aacccgaagt cactcgtgct tttcatcaga
     2641 cgggctgacg tcccatccaa cacgtcaaac acgatcgcca caaggatcgc aatggccgca
     2701 gctccataat cgtctccaaa gacaaacaga atagaggcca ggccgctaaa gagatttccc
     2761 gtggtcagca gattgggaat taaatagaca acccgacgtt ttttgggctg tttcatgaag
     2821 gctccacgca tagcaactcc gccacgatgg atgatcctcc ctttactttc tcgccaacct
     2881 tgacttgaat ggcactatcg tgaggaagat acaaatccat tcgagatcca aaacgaataa
     2941 gtccaaaccg ttcgcctgcc tcaacctgtt caccgggggt gacccaacag acaattcgtc
     3001 tggcaattaa cccggcaatt tggacacaca gtattttttt cccatcggaa cgcctgagca
     3061 tcagcgcatt ttgctcattt tccgctgaag cctccggacg actggctact aaaaaattcc
     3121 cgggagcata gacgacatcc tgtagcactc ctgcagccgg catccggtta atatggacat
     3181 tgaatacatt taaaaaaatg ctgattctga tactgggttc tttgaggaat cggtgctcga
     3241 attcttcttc caccgctacg accgtcccat ctcctggaga cacaatgagg tttttcccct
     3301 gaggaatcgt tctgaccggg tttctaaaaa accaggccgt aaacagggtt aacgatccca
     3361 ttaggcaagt gagccagacc gatccggcca cccaacaccc aagcgtcaga agcccccccc
     3421 cctgcgataa aggggaggcc ttctttggca acaggaattc cttttgcctg atcagccatt
     3481 gggaatgggt ctcacgtccc gcaaccttct catcaatttt tctgcttatc cacaagctga
     3541 tctttttgca gccagggcat catccctcgc aaccgtgcac cgacctcttc aatggggtgc
     3601 tcctctcccc ttctcaagag agcattatac acgggccggt tcgcttgatt ttccaacacc
     3661 cactctctgg cgaatcgacc gctttggatc tctcctaaaa tctctttcat cactttcttc
     3721 gtctcatcgg tcaccacccg tggccctcgt gtcacatcgc catatttggc ggtcgtgcta
     3781 atagaatagc gcatattggc aatgccaccc tgatagatca gatccacgat gagcttcact
     3841 tcatgtaaac attcgaaata ggccatttca ggcgaatagc cggcttcgac caaggtttca
     3901 aaccctgcct gaatcagtga ggtaagccct ccacataaga ccgcctgctc tccaaaaaga
     3961 tccgtttccg tctcttctcg aaaggatgtt tcaatgacac ccgctctggc tccgccaatc
     4021 gcacaggcgt aggccaatcc gaccttagcg gtctgaccgc cgggatcctg gtgaatggct
     4081 aacaaacacg gtacgcctgt ccctttggta tattccgacc tgaccagatg cccaggaccc
     4141 ttcggagcca ccataaacac attcacgtgg ctggcaggtt gaatttgccc aaaatggata
     4201 ttaaacccgt gcccaaaggc cagatatgct ccttgtttta agtttgggcc aatatcttta
     4261 ttgtagatgg cggcttgagc ttcatcagga gctaacagca tcaccacatc ggcacctttg
     4321 accgcatccg cagtcggcat gactttcaac ccagccgctt ccgccttgtt ccatgacgaa
     4381 ccttcccggc agcccacgac cacttgaaga ccactatccc gcatgtttaa ggcatgagca
     4441 tgaccttggc taccgaaccc cactacggcc acagtttttt tggctaaaat ctgtcgatct
     4501 gcgtcttttt cataataaat tttcatcatg cactcctcat aaagataata tagtccttag
     4561 tcgttccgtc gatcagggtg gacggatccc ctcagtctaa aggcagatgt tggttgtcct
     4621 gattaattcg ctttcgcaag cggtcgggat tgggtttgtg tcgagcgtgt cggttcgcgc
     4681 ccaatggcaa tcctgccggt tcgtaccagc tctttgatgc caagtggctg caggagattg
     4741 atgatcgcct ggactttcct cacatctcca gtgacttcaa tggtgtagct tgtaggagag
     4801 gaatccagaa cattagctcg aaaaatatcc gcaatgcgaa gagcttccgc tcgatcctct
     4861 ggccgggtat gaaccttaat tagcgcagtc tctcgttcaa caaactcgct ttcgctgata
     4921 tcaacgactt taatcacatc aatgagtttg ttcagctgct tcaggatttg ttcgataatc
     4981 ggatcatccc ccttggtgac caaggtcatc atggacacag agggatccag cgtcggggca
     5041 actgacaaac tttctatatt aaaaccccgc ccgctgaaga ggcccgctac ccgggacaac
     5101 gatccgaatt tattctctac cgtcaacgaa ataatgtgtt ccataacacc cttttatggt
     5161 tgtactctaa gctgtgatga cagaatccga atccgcttga gtaccaattt tttgcatggc
     5221 attgggcttc tttaagtccg ggggatctgc taataacatt tcatgattac agcctccggc
     5281 cggaatcatg ggataacaat tttcaaactg gtaggtcggg acatcaatca cgaccggacc
     5341 attaatggaa agcgcttcac gaataccgct atccatttcc gaaacctttt caattcgaat
     5401 acctgcagca ccataggctt ccgctaattt cacaaaatca ggaacggtcc ccaggtaact
     5461 tgacgcatac cggccctcat aaaacaaatc ttgccactgc cgaaccatgc catggaaccg
     5521 gttattaata ataaaaactt taaccggtag ttgctcgacg actgcggtcg ccaactcttg
     5581 ggtattcatt tgaatactgc catcaccggc gacacacaga accagtcgat ccggaaacgc
     5641 agcttgcgca cccatcgcgg ccggcaatcc aaaacccatg gtccccagcc ctcctgatgt
     5701 taaccaggat tgaggatttt gtaatttaaa atattgagct gaccacatct ggtgttggcc
     5761 tacgtctgtg gccaaaatag gatttcggtc gctcgtcagc tgatacaggc gatcaataag
     5821 aaattgtggc ttaatttgtc cgtcaggatc ctgatcatag cgcagagggt gcgcttgcct
     5881 ccaggcattc agttgatccc accagggctt tctttgctct ttttgattcc cattcaccgt
     5941 ggcccgaaga atattattca attcgatgag cactcgctta caatcaccga caataggaat
     6001 gtctacctga atatttttcc gaatagaggt cggatcaata tcaatgtgaa taattttcgc
     6061 atcgggacaa aactctgaaa ccttgcccgt cacccgatca tcaaatcggg caccaacggc
     6121 aataaccagg tcagaataat gcatggccat gtttgcgaca taggtcccat gcatgcccag
     6181 catgcccagt gaaaggggat ggtttccggg aaatgcccca agtgccatca acgtcatatc
     6241 caccggaatt tgcgtcagct cggccagctc tttcacctct ggagaggccc cggataacac
     6301 gactcctcca cccacatata aaattggacg cttggctttc atgatcgcgt ccgccgcttg
     6361 tttaattttc caccgacttc cgtcgtacgt ggggttatat ccgcgaatgg aaacagacgt
     6421 cgggtaatga aagtccgctt tatcgagcga aatatcttta ggaatatcga ctaaaaccgg
     6481 gccagggcgg ccagtggtgg caatataaaa cgcctcctta atcgtctgtg ctaaatcttt
     6541 cacgtccttg accaggaaat tgtatttggt gcacggacga ctgagaccga tgttgtcggc
     6601 ctcttgaaag gcatcgttgc cgattaaggc ggtcggaacc tgtcctgaaa agacgactat
     6661 gggaactgaa tccatatacg cgtccgcaag agacgtgata acatttgtca tgccggggcc
     6721 ggaggtaatc agcgctacgc cgggtttgcc catggctttc gcgtatccga cggccataaa
     6781 cccggcacct tgttcgtgtc gtgtcagaat tacccgaata tctttttgct ggtgcaagac
     6841 atcgaatatt ttgagaacca ctccgcctgg taaggcaaac atggttttca cgccctcacg
     6901 ctttaatgct tctaagaaga tttctgcacc agtgattttc atgattctca ccctctg
//
LOCUS       DATIAL010000060         5343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1086980, whole genome shotgun sequence.
ACCESSION   DATIAL010000060 DATIAL010000000
VERSION     DATIAL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5343
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086980"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VLA60_02010"
     CDS             complement(<1..500)
                     /locus_tag="VLA60_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin oxidoreductase"
                     /protein_id="HSF08155.1"
                     /translation="MAAQASFIGQKNKKEQVYTDPWHMMNEAPRTPSFFTGSEVIKEA
                     VRRASCDIMVAYPITPQSEAAALCGELFAEGYIGDYFRGESEFAVMSQCAGAAFGGAR
                     VFTTTAGPGTMRAMENFPMWAGARLPIQMIVTCRGINSPLSIQPDTLEISYLMQTGML
                     VWHAETA"
     gene            complement(641..1267)
                     /locus_tag="VLA60_02015"
     CDS             complement(641..1267)
                     /locus_tag="VLA60_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019385 - methanogenesis, from acetate
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase beta subunit
                     family protein"
                     /protein_id="HSF08156.1"
                     /translation="MTTSYRVMPGPEHFLPPSAASMGIRLPDPGQGHIHGSIVSEEEA
                     LEEAARQFLSAKVPTIFPGPLVLWAWNDKAAKKAKAIRHLYETIKECVDSSCQHAMLI
                     PMPDYRPKYPKINPEIEINPNHPNLTIWHNKIDACMFVGVHCHQANLSLKIIRGGTNC
                     YTIAMCAQAGHEDAMLSVRDATAEKIIRLADWVKKLKGSVAPPAKHLV"
     gene            complement(1634..2068)
                     /locus_tag="VLA60_02020"
     CDS             complement(1634..2068)
                     /locus_tag="VLA60_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08157.1"
                     /translation="MATYLPKLRRVLGMTLMVIFGAIGFIIWQDTQERELEFDRILPS
                     QAQLYHMEVRPGLNSRSFVLTGRLENSAQIFTILSVTIQATIEDCHMTECEIVGQEAA
                     EISLEVPPNQARDFSLTMPFPTIPKIIGEARWRYAILKVRAR"
     gene            2207..3031
                     /locus_tag="VLA60_02025"
     CDS             2207..3031
                     /locus_tag="VLA60_02025"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HSF08158.1"
                     /translation="MPDFSHEIEIASHLARKAGEVIMHIYQNDFAVMYKSIDDPVTKA
                     DQEANALIVDELHTHFPQDSIVAEESPLPSKSLTTGRVWYVDPLDGTKEFIARNGEFS
                     IMIGLTIDAHTKLGVVYWPTRDHLYAGLTDQTAWMEHNGVCQKLSALASKSPTNLSLV
                     TSRSHRSPILSIIQQSLPIHNEKQLGSVGLKIGQIAQGNADLYIEPSPYTKAWDACAP
                     EAILRGAGGCFTDIHGKPIQYGLNNFRNLHGLVGSTPDIHQRVIQALTGRCYSCEG"
     gene            3111..4427
                     /gene="trmFO"
                     /locus_tag="VLA60_02030"
     CDS             3111..4427
                     /gene="trmFO"
                     /locus_tag="VLA60_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HSF08159.1"
                     /translation="MRTDLVIIGGGLAGSEAAWQAANSGARVTLYEMRPKEETAAHKT
                     EHLAEIVCSNSLGSNDPLSAPGMLKEEMRQLHSLIIRIADEVRIPAGTALAVDRDQFA
                     CKVTRALAEHPNIKILREEIHEIPDDALCIIATGPLTSPKLSEAIIQFTQSKNLFFFD
                     AISPIVDADSLNTDRTFMASRYEKGTADYVNCPMDEETYNAFYNALIEAERVQPKSFE
                     NVPYFEGCLPIEVMADRGRQTLLFGPLKPVGLIDPKTGQRPYAVLQLRPENAHGSCYN
                     LVGFQTKLTYPEQRRIFRMIPGLEQAEFLRCGSIHRNTYINTPILLQNTLQVKKQPRI
                     FFAGQLVGVEGYVEAAASGGIAGINAARILSGRAPVTPPPSTAHGALLHYITTCEPKH
                     FQPINSNFGLYPPLDTPVRDKQKKRQLIQERATTHFKAWMTQSELS"
     gene            4427..>5343
                     /locus_tag="VLA60_02035"
     CDS             4427..>5343
                     /locus_tag="VLA60_02035"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HSF08160.1"
                     /translation="MYLQLERGVSPETRRSYQSDLKQFMGHIRHTLSGNDLSEPGVID
                     SLHIRSYLKSLSDQGLKKTSLARKLACLKSFFRFLVEDGRISRNPASTVRSPRQGTRL
                     PTVLTKDEANTLLDSSASQQWVELRNHAILETLYASGARVSELVGLNWADVDLETRSI
                     RLRGKGKKERMVPIGTVAADAILEYQRHPPHKNKIIQPGTSAPRVPYSGSQPLFLNAR
                     GGRLTARSVERMMKQETEHRFTKTVTPHTLRHSFATHLLDEGADLRAIQELLGHASLA
                     TTQKYTHVATDQLMAVYDRAHPRSGSSHS"
BASE COUNT         1450 a         1398 c         1198 g         1297 t
ORIGIN      
        1 gcggtttccg catgccacac cagcatgccc gtttgcatta aataggatat ttctagggta
       61 tccggttgaa tggagagcgg agagttgatg ccccggcagg tcacaatcat ctgaataggt
      121 aaccgtgccc ccgcccacat ggggaaattt tccatagccc gcatggtgcc tggtccggcc
      181 gtggtggtaa atactctggc accaccaaaa gcggctcccg cgcattgaga catgacagca
      241 aattcgcttt cccctcggaa gtaatcacca atatagcctt cagcaaagag ctctccgcac
      301 aaggcagcag cttcactttg aggggtaatg ggatacgcga ccattatgtc acaggaggct
      361 cgccgtacgg cctcttttat gacttcactc ccggtaaaga aagacggtgt tcttggcgcc
      421 tcattcatca tgtgccaagg atcggtatac acctgctctt ttttgttttt ttgtcctatg
      481 aaagaagctt gagcggccat gattttgacc tcctttatct gcttcagggt gaaagacttc
      541 cgattttctt aaacgataat atgaataagg aaaatcatag atgaaagttt ctcagaacaa
      601 gttattcatc tctttcatgt atgatgaaaa cccagaacaa ttacactaaa tgtttcgctg
      661 gaggggcgac cgatcccttc aatttcttca cccaatccgc caatctgatt atcttttcgg
      721 ctgtcgcatc acgaacggat aacatcgcat cttcatgacc agcttgagca cacatggcaa
      781 ttgtgtaaca gtttgtgcca ccacgaataa tcttgagaga aagattggcc tgatggcaat
      841 gaacgcccac aaacatgcag gcatcaattt tattatgcca gatagtcaaa tttgggtggt
      901 ttggatttat ttcgatttca ggattaattt taggatattt cggtctatag tccggcatgg
      961 gaatcagcat ggcatgttga caagaggaat ccacgcattc tttgatggtt tcatacaaat
     1021 gacggattgc tttggccttc ttggcggcct tgtcattcca tgcccaaaga actaatggtc
     1081 caggaaagat cgtgggcact tttgccgaaa gaaactgcct ggccgcctcc tccaacgctt
     1141 cctcttcgga aactatgcta ccatgaatat gtccttgtcc tggatcggga agcctaatcc
     1201 ccattgaggc cgcagacggt ggcaaaaaat gttcggggcc tggcattacc ctataagaag
     1261 ttgtcatcgt accctcatgt ctccattaaa aaaaattgga caaatactat ccgctccacc
     1321 ttcttactgt aagaaggcaa tgatgaaatc tcaagatcga aaaagaccca cctgtccaaa
     1381 atattggact ttttgagaat atatctatga gtcttttgcc ctataaatca aaattttcct
     1441 gactaaccta ggctagtata agaaggcgct ataaatattg tcaatttgaa tattgttgat
     1501 aacagacgga aaaaccgatt tcccagagtg agagtcctca tggctgtcaa cctgttcatt
     1561 ggtttgaaaa ggaaaagacc taaaaggaat gaggagagag aaaacccacg gcaggcaaaa
     1621 cggaaaacat ggcctatctg gctctgactt tcaaaatggc ataccgccac ctcgcttctc
     1681 caataatttt tggaattgtg ggaaagggca tggttagaga aaaatctctc gcctggttcg
     1741 gaggaacttc caaagagatc tctgcggcct cttgcccaac gatttcacat tctgtcatat
     1801 ggcagtcctc aatcgtagcc tggatagtca cggaaagtat ggtaaagatt tgagctgaat
     1861 tttcaagcct cccagtcagc acaaacgacc gggagttcag tccgggtcga acctccatat
     1921 gataaagttg ggcctggctg ggaagaatcc ggtcaaattc caactcccgc tcctgagtat
     1981 cctgccaaat aatgaacccg atagctccaa agatgaccat cagggtcatc cccagaacgc
     2041 gacgcaattt tggcaaatac gtcgccatga aaatcatgat taatatcaac agaataatgg
     2101 caataggcag cataaatttc acaggcagtg ggtgggagaa aaactcttct tactctctac
     2161 tcctcttatc ggttttctga atccccttct ttcaccggag gaagcaatgc cagatttttc
     2221 tcatgaaatt gaaatcgcct ctcacctggc tcggaaggca ggggaggtta tcatgcacat
     2281 ctatcaaaat gattttgcgg tcatgtacaa aagcattgac gatcccgtca ccaaggccga
     2341 tcaagaagcc aatgccctta tcgtagatga attacacacc catttcccac aggattccat
     2401 cgtggcggag gaaagcccgc tcccctccaa atctttgaca acaggccgag tctggtacgt
     2461 ggaccccctg gatggaacaa aagagtttat tgcacgcaac ggggagtttt ccatcatgat
     2521 cggactgacc attgatgctc acaccaaact tggcgttgtc tactggccta cccgtgacca
     2581 cctctatgcc ggcctgacgg atcagacggc ttggatggaa cacaacgggg tttgccaaaa
     2641 gctctcggct ctcgcatcca aaagtcccac caacctctca ctcgtcacct ctcgatccca
     2701 tcgatctcca attctttcta tcattcaaca atcattgccc atccataatg aaaaacagtt
     2761 gggaagcgtg ggactgaaaa ttggccaaat tgcccaagga aacgctgacc tctacattga
     2821 accgagtccg tataccaagg catgggatgc ctgtgcgcct gaagccattc tgcgaggagc
     2881 cggcgggtgt tttaccgata ttcacgggaa acccatccaa tatggactca ataactttcg
     2941 caatcttcat gggttggtcg gaagtactcc agacattcat caacgtgtca ttcaagcctt
     3001 gacgggtcgg tgttactcgt gtgaaggttg acaccttttt ccggcaattc ttagaattcg
     3061 ttccttaata ctttctcagc gcctttgacc actatcactc tcacacaatc atgagaacag
     3121 atcttgttat tatcggaggc ggcctagccg gatcggaagc ggcttggcag gcggccaact
     3181 ctggagccag agtgaccctg tatgaaatgc ggcctaagga agaaacagcc gcacataaga
     3241 ccgaacacct ggccgagatt gtctgctcaa attctttggg gtcgaatgat cctctcagcg
     3301 ctcctggaat gttgaaagag gaaatgcggc aattgcactc cttgatcatc cgaattgccg
     3361 acgaggtgcg gatcccggca ggaacggcat tagccgtgga tagagatcaa tttgcctgca
     3421 aggtgacccg ggcattagcc gaacacccga atatcaaaat tcttcgagaa gagatccatg
     3481 aaattcccga tgatgcgctg tgcataattg ccaccggccc cttgacctca cccaaattgt
     3541 ctgaggcaat catccagttc acccaatcga aaaacctgtt tttcttcgat gccatctccc
     3601 caatcgtgga tgcggattca ctcaacacag accggacatt tatggcatca cgatatgaaa
     3661 agggaaccgc ggattacgtg aattgtccta tggacgaaga aacctataac gccttctata
     3721 acgcattgat cgaagccgaa cgcgtccagc caaagtcttt cgagaacgtg ccctattttg
     3781 aaggatgtct cccgattgag gtcatggccg accgtggccg gcaaacctta ttgtttggtc
     3841 ccctcaagcc cgtgggatta attgatccca aaaccggcca acggccctat gctgtgctac
     3901 aattacgtcc agaaaacgct catggctcct gctataatct ggtagggttt caaaccaaac
     3961 tcacctaccc ggaacaacgc cgaatcttca gaatgatccc tggcttggag caggctgaat
     4021 ttttacgatg tgggagcatc catcgaaata cgtatatcaa tacaccgatc ctcctacaaa
     4081 acaccttgca ggtcaaaaaa cagccacgca tcttttttgc cggacaattg gttggagtag
     4141 aaggttatgt ggaggctgcg gccagtggag ggatagccgg aatcaatgcc gcccgcatcc
     4201 tatcagggcg tgctccggtc actccaccgc cctctaccgc ccatggagca ctgctccatt
     4261 acattacgac ttgtgagcca aagcactttc aaccgattaa ctcaaacttt gggctatacc
     4321 cgccacttga tacaccagtg cgagacaaac agaaaaaacg acaactcatc caagaacggg
     4381 cgactaccca tttcaaagca tggatgacgc aatccgagct ttcgtaatgt accttcaatt
     4441 ggaacgggga gtttcgcctg aaacacgccg aagttatcag tccgacctca aacaattcat
     4501 ggggcacatt cgacacacgc tttccgggaa cgatctttct gagcctggcg tcattgactc
     4561 actccatatt cgatcctatc tcaaaagcct cagcgatcaa gggttaaaaa aaacctccct
     4621 ggccagaaag ctggcatgcc tcaaaagctt ttttcgtttt ttagttgaag acggacggat
     4681 ttcgcgtaat ccagcatcca ccgttcgttc gcctcgacaa gggacacgtc ttcccaccgt
     4741 cctcaccaaa gacgaagcca ataccctgtt agattcgtca gcatcccagc aatgggttga
     4801 gttgagaaac cacgccattc tggaaacgtt gtatgcaagc ggggctcggg tatcagaatt
     4861 agtcgggtta aattgggcgg atgtggatct agaaacccga tcgatccgac tgcggggaaa
     4921 agggaaaaaa gagcggatgg tgcctattgg cacagtggct gctgatgcca tcttggaata
     4981 tcaacgtcat ccgccccaca agaacaagat tatacaaccc ggaacatcgg ctccacgggt
     5041 gccctactcg ggatcgcaac ctctttttct caatgctcga ggaggacggt tgaccgctcg
     5101 aagcgtggaa cgcatgatga agcaagagac cgaacaccgt ttcactaaga cggtcactcc
     5161 ccataccctg cgccattcct ttgccaccca tctcctcgat gaaggagccg atttgcgagc
     5221 catccaggaa ctcttgggcc atgcctcgct cgccacaacc caaaaataca cacatgtggc
     5281 aacagaccaa ctcatggccg tttatgaccg ggcacacccc cgatccggct cttctcactc
     5341 cat
//
LOCUS       DATIAL010000061         5485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1517189, whole genome shotgun sequence.
ACCESSION   DATIAL010000061 DATIAL010000000
VERSION     DATIAL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517189"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VLA60_02040"
     CDS             complement(<1..547)
                     /locus_tag="VLA60_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08161.1"
                     /translation="MKAEAETLEFVTINGLLTYGEVIARRPPVEGAEPHPPLASLEDR
                     PQRVLEAMAALRLFVRDAQKGYANAAAYREARQALIQQACGGDELVFFAAWNQLLAQG
                     ELSPLFRAPIGATNKPIRRRPVAIVPREHMTPNLAEGRIVLDIGDDRYWLMPRDLSDR
                     TLFFTMRHGVSRMDSKKFRVGR"
     gene            1058..2458
                     /gene="pyk"
                     /locus_tag="VLA60_02045"
     CDS             1058..2458
                     /gene="pyk"
                     /locus_tag="VLA60_02045"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSF08162.1"
                     /translation="MQKTKIICTIGPVTESYEMLRKMYDAGMNIVRLNMSHGTHESHA
                     KVIKHIKTLNTKVPFPIPILLDTQGPEIRTGDLSIDLNLQEGTIVSISARGPMDVEES
                     SIHINYADLMESVNVGDKITVDNGLINFEVLEKQDRLMQCRVLDGGVLKSKRHVNLPG
                     IRVNLPAITQKDEKDIAFGLAADVDFIALSFVREAKDIQQLKQLMGHKVGRVKIIAKI
                     EDQEGVRNLEDIIRESDGIMVARGDLGAEINLEDLPNVQRRIVRLCAESGKRVIVATH
                     LLESMIHNPIPTRAEVTDVANAIYEEVDGVMLSGETTVGKYPLKCIEYLRKISIKTEA
                     IPGLQFAKQLKLTTDKQQLATAAVQLAEGLRAKGIVVITRRGIMADLVSNCRPFSTNI
                     YAFTNGSQSRRTMTLNRGVYPFRIDFSSDPEKTLQTAFRILKTREHFQIGDKVVIISD
                     VLAQERVDGIQIRAIP"
     gene            complement(2893..4131)
                     /gene="coaBC"
                     /locus_tag="VLA60_02050"
     CDS             complement(2893..4131)
                     /gene="coaBC"
                     /locus_tag="VLA60_02050"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HSF08163.1"
                     /translation="MSTELAGKRIILGVTGSIAAYKAVGLLRLLTQAGAGVHVVMTDS
                     ATRFIAPLTFEVLSQNPVASDVFAGHQDMKHLSLTEQADLLLVAPCTANTLAKLALGL
                     ADNLLGTLSLTVQCPVMICPAMDGEMWAHPAVQAHAQTLRHRGVVLVEPEVGALASGE
                     WGEGRLAAEESIMSTAVDLLRRRTDWQAERVLVSAGPTQEPIDPVRFISNGSSGKMGF
                     ALAEAAAARGAEVVLVTGPTQLMAPKNVTVIPVVTAEDMFQALTARFEWATTLLMTAA
                     VGDFRPKQAYAQKVKKDQWQGEGLDLERTPDILMALSAQRTHQRLIGFAAETDHLIEH
                     GQAKLAGKHLDMLIVNHVGGEQSAFGNDTNEVYVLTPGAAPHHIPRMPKRQLADTLLD
                     RIVSLPVQPFRQKTLSSPTS"
     gene            complement(4162..4530)
                     /gene="rpoZ"
                     /locus_tag="VLA60_02055"
     CDS             complement(4162..4530)
                     /gene="rpoZ"
                     /locus_tag="VLA60_02055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HSF08164.1"
                     /translation="MDALSLLPQHHQKEFDSRYRIVLIAAQRAKQLIRGGEPYGASKF
                     SKDTSRALEEVLNGQVPYLIGQEAKEAIKNAKRANERPIDPALYAQPDENAQEIKKEL
                     SVYIDDTAQHLGTVVEPEEA"
     gene            complement(4611..5216)
                     /gene="gmk"
                     /locus_tag="VLA60_02060"
     CDS             complement(4611..5216)
                     /gene="gmk"
                     /locus_tag="VLA60_02060"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HSF08165.1"
                     /translation="MGVTGKKLLFVVSGPSGVGKSTLCRHILKTIPDIRLSVSYTTRK
                     PRSGETDGKEYKFISETEFHTKISENAFAEYAEVYGRLYGTPWKELEQESGSNTDILL
                     DIDVQGAREVMKTLQKAVTVFILPPSLEVLRTRLVERATDTTDEQERRFQKSQDEMRS
                     YSEYQYTIRNETLEQAIEELQSVIVAERVRTTHIDAAHVFR"
     gene            complement(5246..>5485)
                     /locus_tag="VLA60_02065"
     CDS             complement(5246..>5485)
                     /locus_tag="VLA60_02065"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HSF08166.1"
                     /translation="AERSDVTEELTRLQSHVTQFRSTLKSKGPVGKRLDFLLQEMGRE
                     VNTIGSKASDSEISGQVVELKSELEKIREQVQNIE"
BASE COUNT         1326 a         1487 c         1325 g         1347 t
ORIGIN      
        1 gtcgccccac acggaacttc ttactatcca tccgggagac gccgtgccgc atcgtgaaaa
       61 acagcgtgcg gtcacttaaa tccctcggca tcaaccaata gcggtcatcg ccgatatcaa
      121 ggacaatccg accttcagcc agattgggcg tcatatgctc ccgcgggacg atcgccacgg
      181 gccgacgtcg gatcggttta ttcgtggccc ctataggtgc ccgaaataac ggagaaagct
      241 caccctgagc gagcagttga ttccacgccg cgaaaaacac caactcatct cccccgcagg
      301 cctgctgaat cagggcctgt ctggcctctc tgtaggccgc agcattggca tacccttttt
      361 gggcatcccg gacaaacagt cgtagggcgg ccatagcctc caacacgcgt tgcggacgat
      421 cttccaagga agccaacggg ggatgaggct ctgctccctc taccggcggt cgcctcgcaa
      481 tgacctcgcc ataggttagc agcccattaa ttgtgacaaa ttccagagtc tcggcttcgg
      541 ctttcatcta ttcacctctt ccaatccttc agcgattctt gatctcatat aaatcttgtc
      601 ccatcatcat aatattatcc cacctgatcg acaacacaag tccccgccat agacgtctga
      661 tctgtctcct accgatacga tcattctatg catataggtt ctcacggttc gtgggaaagg
      721 tttccttctt tcactcacca ggtatggaga acccatcccg accgaccctg gtcagtgatc
      781 gaccctgcta gagtttgacg ctaaagtggg ctacacttca tgagaaccca cagggaggtg
      841 ggatatcgcc tgcaccaaac agcactccag caacacgggt acctctaatc ctatcaatct
      901 caaacaggag ttgggcgcct ccccatctcc ttgggcctct gcgtctgatg ccgctctcac
      961 aggaggcaag ggtgccatag gattcaactg acgaatttga agatagtggg attgctttca
     1021 caattgacga ttattgacaa ctgataggga aaccttcatg caaaagacca aaatcatctg
     1081 cacgatcgga ccggtgaccg aatcgtatga gatgttgcgc aaaatgtatg acgccggtat
     1141 gaacatcgtg cggttgaata tgtcccatgg cacccacgaa tcccatgcca aagtgatcaa
     1201 acacattaag acgctcaata ccaaggttcc ctttcctatt ccgattcttt tagataccca
     1261 aggccctgaa atccggacag gagatttatc gattgatctc aatctccagg aaggcacgat
     1321 cgtttcgatt tctgcgcgtg ggccgatgga tgtggaagaa agctctattc acatcaacta
     1381 tgccgattta atggaatccg tgaatgtcgg cgataagatt accgtggaca atgggttaat
     1441 caattttgag gtccttgaaa aacaggatcg cctcatgcag tgccgggtcc ttgatggggg
     1501 ggtcttgaaa agcaaacgcc atgtcaattt accgggaatc cgcgtcaatc tccctgcgat
     1561 tacgcaaaaa gacgaaaagg atattgcctt tggtttagcc gccgatgtgg atttcattgc
     1621 tctgtccttt gttcgggaag cgaaggatat ccaacaactg aaacagttga tgggacacaa
     1681 agtggggagg gtgaaaatta ttgccaagat tgaagatcag gaaggggttc gcaatctgga
     1741 agatataatc cgggaatcgg atggcattat ggtggctcgc ggagatttgg gggcagaaat
     1801 caacctggag gatctgccga atgtgcagcg cagaattgtg cgactctgcg cggaatccgg
     1861 caaacgcgtg attgtggcaa cccatctcct ggaatcgatg atccacaatc ccatccccac
     1921 gcgtgccgag gtcactgatg tggcgaatgc catttatgag gaggtcgacg gggtgatgtt
     1981 atccggagaa accacggtcg gaaaatatcc gctgaaatgc atcgagtatc tgcgaaaaat
     2041 ctccatcaaa accgaagcca ttccaggttt gcagtttgcg aaacagttaa agctgacgac
     2101 agacaaacaa caattagcca ccgctgccgt ccagcttgcg gaaggactac gcgccaaagg
     2161 gattgtcgtg atcacccgac gaggtatcat ggcggatttg gtctcgaatt gtcgtccatt
     2221 ttccacgaac atttatgctt tcacgaacgg gagtcaatcc cgtcgaacga tgacgctcaa
     2281 tcgaggcgtc tatccctttc ggatcgactt tagttcagac ccggagaaaa cgttacaaac
     2341 cgcatttcgg attctaaaaa cccgtgaaca tttccaaata ggagacaagg tcgtgattat
     2401 ctcagacgtg ttggctcaag agcgggtcga tgggattcaa atccgagcca ttccctaagg
     2461 caggccgttg caggccattt gatcagccca gccacccgtc aggaataccg attcaggtgg
     2521 ggccctttcc tgacacgttc ctgttggaag gcttccaaga aaagccgata ctttttttct
     2581 ggaccgggcc gggggagagg agctagattc ttcgaatagc cagaagaatc atggaaataa
     2641 tccaggcgaa aaagacgata ttgatgaaaa atccttccat actattctta tcggcttatc
     2701 agctcctaaa gttaagtttt ttcttgttct ctgaggttct tgcttcattt ttcactcatt
     2761 tccgccatct taacccaaaa atccattaag acccttgagc tttcaccaca caagagatgt
     2821 cctttggctt gggagacgtt gttccatcgc aatggaaaag ggtgaggaga caccccatca
     2881 ggtgagcact aactatgagg tcggtgaaga cagagtcttc tgcctgaaag gctgaaccgg
     2941 aagggacacg atacgatcta ggagcgtatc ggcaagctgt cgtttcggca ttcgcgggat
     3001 gtggtgcggg gcagcacccg gcgtgagcac atacacctca ttggtatcat ttccaaaagc
     3061 agactgctct ccgcccacgt gattcacaat cagcatatcc agatgtttgc cggcaagctt
     3121 ggcctgtcca tgctcaataa gatgatcggt ttcagccgca aagccgatca ggcgttggtg
     3181 ggtacgctgg gcagagagtg ccattaaaat atccggcgtt cgttccaaat ccaatccttc
     3241 gccctgccat tggtcttttt ttactttctg tgcatacgcc tgtttggggc gaaaatctcc
     3301 caccgccgcc gtcatcaaaa gcgtggttgc ccattcaaat cgtgccgtga gcgcctggaa
     3361 catgtcttcg gcggtgacca ccggaatcac ggttacgttc ttcggagcca ttaactgggt
     3421 gggacccgtc accaagacga cctcggcccc tcgtgcggcg gctgcctcgg ccaaggcaaa
     3481 tcccattttg cctgatgacc cgttactgat aaatcgcacg gggtcaatgg gttcctgggt
     3541 tggccctgcc gataccaaga cacgctccgc ctgccaatcc gttcggcgac gcaacaggtc
     3601 aacggcggtc gacatgatgc tttcctccgc agctaaacgt ccctctcccc attctccaga
     3661 ggccaaggcc ccgacttccg gctcgaccag gacgacccct cgatgacgga gggtctgagc
     3721 atgggcttgg accgccggat gggcccacat ttctccatcc atcgccggac aaatcatcac
     3781 cggacattga acggtgagcg aaagggttcc gagcaaattg tcggctaacc ccagagccaa
     3841 tttggctaac gtgttcgcgg tacagggagc cacgagcaac aaatcggctt gttccgttaa
     3901 ggacaggtgc ttcatgtcct gatggccggc aaagacatca ctcgccaccg gattctgcga
     3961 cagcacttca aatgtcagag gggcgataaa ccgggtggct gaatccgtca tgacgacatg
     4021 cacgccggca ccggcttgcg tcaacagacg taggagcccg accgccttat aggccgcaat
     4081 acttcccgtc acccccagga taatccgctt gcctgctaac tcggtgctca tcttctgcat
     4141 tctttcgcca agtgctttca gctaggcttc ttcaggttcg accaccgtgc caagatgttg
     4201 agccgtgtca tcaatataca cactcaattc ttttttgatc tcttgcgcgt tttcatccgg
     4261 ctgtgcgtaa agagccggat caatgggacg ttcattcgcg cgtttggcat ttttaatggc
     4321 ctctttggct tcctggccaa ttaaatacgg aacctgcccg ttgagcactt cttccagggc
     4381 tcttgaggtg tctttcgaaa atttactggc tccatacggc tctccacccc gaatcaattg
     4441 tttggcccgc tgggcggcaa tcagcacaat ccgataccgc gaatcaaatt ctttttggtg
     4501 atgttggggt aataacgata atgcatccat tgaatatcct ctttcctttc tgtgagaaac
     4561 acacgttcct ggtgaatagt ctacacaaat cgatcccatc aatgcagcaa ttatcgaaac
     4621 acatgcgcgg catcgatatg ggtggttcga acacgttcag ccacaatgac ggattggagt
     4681 tcctcgatgg cctgctccaa ggtttcattg cgaatcgtat attggtattc cgaatagctc
     4741 cgcatctcgt cctgggattt ctgaaaccga cgctcctgct cgtccgttgt atccgtcgct
     4801 cgttcgacca gacgcgtccg aagcacctcc aatgaaggag gcagaataaa cacggtcacg
     4861 gctttttgaa gcgttttcat gacctcccgc gcaccctgga catcgatatc caataagata
     4921 tccgtattcg aaccggactc ctgctccaac tccttccagg gtgttccgta taatcgtcca
     4981 tagacttcag catattcagc aaacgcgttc tcggaaattt ttgtgtgaaa ctccgtttcc
     5041 gaaataaact tgtattcctt gccatccgtt tcgcctgatc ggggcttccg cgtggtgtag
     5101 gaaaccgaaa ggcgaatgtc gggaatcgtc ttcaaaatat gccggcagag cgttgacttc
     5161 cccactccgg aaggacctga tacgacaaat aacaattttt ttcccgtgac tcccaccgtt
     5221 cctccgcagt tatgacatag attgattatt caatattttg gacctgttca cggatttttt
     5281 ctaattcact ctttaactcc acaacctgcc cggagatctc ggagtcgctc gcctttgatc
     5341 caatggtatt gacttccctt cccatttctt ggagaagaaa atcaagtcgc ttgcccactg
     5401 ggcctttgga tttgagtgtg gaacgaaatt gtgtcacatg actctgcaac cgtgttaatt
     5461 cttccgtcac gtccgatcgt tctgc
//
LOCUS       DATIAL010000062         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1409748, whole genome shotgun sequence.
ACCESSION   DATIAL010000062 DATIAL010000000
VERSION     DATIAL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409748"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VLA60_02070"
     CDS             <1..1064
                     /locus_tag="VLA60_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSF08167.1"
                     /translation="TYCNEQFGAINGTCNHVGGPLGEGTLEGEYVVCPWHYWKFHYRT
                     GKGEPGYVEDAVPAYDVKVENGRVLIRSTPISKRTRQAHEPHPLARPPRREDGPIRVL
                     GLSTTVMTPDHPRYSTSEDLLEVGLKHASDNLECETRLLRLRDLSFRPCEGFYSKSSR
                     ACTWPCSITQMDPQDEMDQVYEGLVHWADVLVLATPIRWGAASSLYFKMVERMNCIQN
                     QETISDTHLLQNKVGGFIITGGQDNIQAVAGQLLGFFAEIGVQFPQFPYIAHSRGWSA
                     EDMQNNMRYVQKSRSLREGAMALVERCVQMARLLVHGDLGECRTARGGRKAHELDTRA
                     QLHGSEDGEKIQGSSRNKF"
     gene            1528..2439
                     /locus_tag="VLA60_02075"
     CDS             1528..2439
                     /locus_tag="VLA60_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08168.1"
                     /translation="MYASQSLHTPLDQLFSFYRKQVVACLKPLVLEYYPDLFTHRQDE
                     YRKMMELSAKMTVVGHACAEIAGFPYDARRQMIGSLFGGCCFLADSFIDDFGLDVANS
                     YIDRMDDLLTKGWFEIQTDREKLFYVIIARLFAERDVLDPLLRQAILLLFQAQKRDVA
                     FRSDPESIRSRPRREQLRILRESARNRSGHAILVLTGFLVPVVPLHYVSMIFLAGSLI
                     MHIDDHGDCYSDLHYHRLTYLNQVADPERTLRRIFHGHIARLRQGLPEGSGRDLLIAF
                     LTKYFSTRLKKHRLQKTHRELAWAVYD"
     gene            2556..>2916
                     /locus_tag="VLA60_02080"
     CDS             2556..>2916
                     /locus_tag="VLA60_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08169.1"
                     /translation="MGRSPEGTTGTTEVYWGDVFAIALQELRSQGKVSDALPLLRIDG
                     QAEAAMAPYQFDPVYGKLHRRECSNIADDSRSALFARWSMGRDEQFLACSHCRPHPYP
                     RGQEAQDVTLDILFGVIS"
BASE COUNT          670 a          689 c          781 g          776 t
ORIGIN      
        1 taacttactg caatgagcag ttcggagcga tcaatggcac gtgcaaccat gtgggcgggc
       61 cgttgggtga aggtacgttg gagggagagt atgtggtctg cccctggcat tattggaaat
      121 tccattatcg gactggcaaa ggggaacccg ggtatgtaga agatgcggta ccggcctatg
      181 acgtgaaagt agagaacggg cgggtcttga ttcgatccac accgatttcg aaacgaacgc
      241 gtcaagcaca tgaaccccat ccgcttgctc gacctccccg cagggaggac ggtccgattc
      301 gggttctcgg actttccacg accgtcatga cgcctgatca ccctcgctac agtacttcag
      361 aagatctgct tgaagtcggg ctgaagcatg cttcggataa tctagaatgt gaaacgcggt
      421 tattgcgtct tcgggatctg tcctttcgcc cctgtgaagg tttttattcc aaatcatcac
      481 gggcttgtac ctggccctgt tcgattacgc agatggatcc ccaagatgag atggaccagg
      541 tctacgaagg gctggtccat tgggctgatg tcctggtact ggccactccc attcgatggg
      601 gggccgcaag cagcctttat ttcaagatgg ttgagcggat gaactgtata cagaatcaag
      661 aaacgatttc agatacccat ctgcttcaaa ataaagtcgg cgggtttatc attaccggcg
      721 ggcaggataa tattcaggcc gtggccggac aactactcgg atttttcgcc gaaatcggtg
      781 tgcagtttcc gcagtttccc tatattgccc attcccgggg atggagtgcg gaggacatgc
      841 aaaataatat gcgctatgtg cagaagtccc gatcactccg ggagggcgcg atggccctgg
      901 tagaacgctg cgttcaaatg gcccgcctcc tggtacacgg agatttgggg gaatgccgga
      961 cggcgagagg cgggcgaaaa gcccacgagc tggacaccag ggctcaactg catggctctg
     1021 aagatggcga aaaaatccag gggtcaagcc ggaataagtt ctagctagga tgacagccct
     1081 tctcgtcaat gctgaactct ggcctatccc tatcttctaa ttatttgaca acatgcaacc
     1141 caccgattgt tgtttatcgc caggaatctc agcttctatg agatttcaaa tatgatgttg
     1201 acgcatgtcg catcagtgca gtgaagttga atgcctacaa cattcgcaca cggatcccgt
     1261 gagtcttttt tccggtagag tccggttttg ccggaatcct gatccaagcc tcccatttgg
     1321 tggagatttt gtatgaaaca cgaatctggc tccactcaca gttatccaca ctggcatggc
     1381 tgcctaaagg cataaccgtt gcattagttg ccttatacgc tctgctttaa ttctgaaatt
     1441 ttttgtcagg attcagtgga taatatttaa agtttgattt caacgcttta taatagagcc
     1501 cgttcgttcc gacgagaagt ttttctcatg tatgcgtccc aatccctcca tacgcctctt
     1561 gatcaattgt tcagttttta tcggaaacag gtggtggcct gtctgaagcc gttagtgctg
     1621 gagtattatc ccgacctgtt tacccaccgg caggatgaat accggaagat gatggaattg
     1681 agtgccaaaa tgactgttgt gggccatgcg tgcgcggaga ttgccggatt tccctatgat
     1741 gcacgtcggc aaatgatcgg cagtttgttc ggcggctgct gttttcttgc ggatagcttt
     1801 attgatgact ttggattaga tgttgccaat tcctatattg atcggatgga tgacctgttg
     1861 accaagggtt ggtttgagat tcagacggat cgagaaaaac tgttttatgt cattattgcg
     1921 cgtttatttg ctgaacggga cgtcctggat cctctgcttc ggcaagccat actgttgctc
     1981 tttcaggcac aaaaacggga tgtggccttc cgttctgatc cggagagcat tcgctcacgt
     2041 ccccgccgag aacaattgcg aatcttacgg gaaagcgcca ggaaccgaag cggtcatgct
     2101 attttagttc tcacaggatt tttagtcccg gtggttcctc tgcattatgt aagtatgatc
     2161 tttctcgcag gctccttgat catgcatatc gacgatcacg gggattgtta ttcagacttg
     2221 cattaccatc ggttgactta tctcaatcag gttgccgacc ccgaacgcac tcttcgtcgg
     2281 atctttcatg ggcatattgc ccgtttgcgg caggggctac ctgaaggctc agggcgggat
     2341 ttgctgattg cctttttgac caaatatttt tcgacacgtc tcaaaaaaca tcgtttgcaa
     2401 aaaacgcatc gagagttggc ttgggcggtc tatgattaag ctcccaggaa aaatcatctc
     2461 gaccgtggtg gaagtggtgt gtccggttgg tggtattttc acggaggcaa acgttcaagg
     2521 aagagggggc ccggtggtca ggaacacaga gaaatatggg acgttctcct gagggtacaa
     2581 cgggaaccac ggaagtgtat tggggtgatg tgtttgcgat tgcccttcag gagctacggt
     2641 cccaaggaaa agttagcgat gcgctccccc tgttacgcat tgatggccaa gccgaagcag
     2701 ccatggcccc gtatcaattc gatccggttt atgggaaact tcatcgtcgg gaatgttcaa
     2761 atattgctga cgattcccgg tcggcgcttt tcgccagatg gagcatgggg agagatgaac
     2821 aattcctggc ctgttcccat tgccgtccgc acccctatcc tcgtggccag gaagcacagg
     2881 acgtaacgtt ggatatttta tttggtgtca tctcca
//
LOCUS       DATIAL010000063         5687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_227231, whole genome shotgun sequence.
ACCESSION   DATIAL010000063 DATIAL010000000
VERSION     DATIAL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227231"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VLA60_02085"
     CDS             complement(<1..259)
                     /locus_tag="VLA60_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08170.1"
                     /translation="MALNSLDFRQTWLWQHVFVNPRLDADITEQDYFRVQFLTMRDRV
                     GHLVSRIAADLPNMTVHDLTHLDFLWETTSLIAEGAVSVSPP"
     gene            985..1386
                     /locus_tag="VLA60_02090"
     CDS             985..1386
                     /locus_tag="VLA60_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08171.1"
                     /translation="MMKKLFAVYGQLKRRVFVLLIVSLFLIAPFALAGPSSLRIILGT
                     FSPYYSPKFAHIRTGTLISWENPTADLHSITHDGCKTSERCAFDSGPLGPNRTFTLRH
                     LPPGQYPYHCSFHPIMQGTLVVTGPDTSGET"
     gene            complement(1920..2417)
                     /locus_tag="VLA60_02095"
     CDS             complement(1920..2417)
                     /locus_tag="VLA60_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08172.1"
                     /translation="MEKELSLVKIGLALVLLGLLFGVGMGIVFGVNEDVFKDYIAQGI
                     TANPGVHDSKSADKIWRYAQRAHFHATGIAAFSLALVILVMFSTLKPTYKKASAILLG
                     LSSFYPLSWYTMFWLAPSIGRGPAHGHILTEFFAYTGVGGLLLGAFFLCANVFLGSFR
                     DKPRT"
     gene            complement(2738..2878)
                     /locus_tag="VLA60_02100"
     CDS             complement(2738..2878)
                     /locus_tag="VLA60_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08173.1"
                     /translation="MFVYFIMPSRRVHLQADDSHPWSNLVGMSYTQPPVTREDLTPAL
                     ET"
     gene            2941..3480
                     /locus_tag="VLA60_02105"
     CDS             2941..3480
                     /locus_tag="VLA60_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HSF08174.1"
                     /translation="MGVICLMLGACSSVEPAGKLPTALSVDLSRYSGMWHEIARLPMW
                     GQRNCVRSTAEYRLLEAGKVAVRNACTTSTGEEISIEGVATVVDPEHQAKLNVVFDQW
                     AAKLVALLTSSEQGNYWILRVDPDYRLAIVGTPDRDYLWILARTPSLDEATYQEAVAF
                     SHRLGFQIEHLIRAPISAD"
     gene            complement(3677..4693)
                     /locus_tag="VLA60_02110"
     CDS             complement(3677..4693)
                     /locus_tag="VLA60_02110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSF08175.1"
                     /translation="MSHQHNMKKNIRGEFLRPGPLVFHNQQASAGLKGFLLRACILTL
                     VFSGVGTLTHAFDLGDALKQLGQPDETTTGKTQDGTPQKQTGKKTPSLTDLTDLVKGT
                     STEEEIAIGQEIAGRLLGAAPLVKDERLQRYVNQVGKWLSLQSGRTDLDWYFGVIDSD
                     DINAFAAPGGFIFLTKGLYHQLHSEAELAGVLGHEIGHVIKQHHLTIIKKSQAIDMGS
                     SLFSQKLGKMKNEQAKQAVNNLIGSGAEVMARGLDKDAEYQADRIGVVLAARAGYDAY
                     GLPMVLQEIGHAGLNDSSVALLFKTHPHPDTRLAELGDSMGETFDKYSHGKIVKDRFY
                     TLSK"
     gene            complement(4697..5209)
                     /locus_tag="VLA60_02115"
     CDS             complement(4697..5209)
                     /locus_tag="VLA60_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08176.1"
                     /translation="MKWLAFLTIVMIGVFPAEGKASDPGVAVRTCQVMSEPFNDARVV
                     LSLKEGDSVEILRRKGGWLEMSRKGKTGWVRMLYIRRGGASEKVSAVTEASGVLGLAT
                     GRAGTGNVVAATGVRGLDEEELKEAEFNEQELQTLKTYRTSKKQAQEFANQAGLQVQK
                     VPFMQPTDGN"
     gene            complement(5295..>5687)
                     /locus_tag="VLA60_02120"
     CDS             complement(5295..>5687)
                     /locus_tag="VLA60_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08177.1"
                     /translation="PERLEEIPEGRFLTLLKRGLYGKKVGFTIEPFSRKPAGIEKAFQ
                     NMFLIYWVENHVETKPWEVEKSGEQTVSLVREVVRSICKNDRIGCKLGWCQGPLTYGN
                     ENSRLEAWQHLVIKAMVNLKEQPLEITA"
BASE COUNT         1471 a         1481 c         1217 g         1518 t
ORIGIN      
        1 caggggggga cacactgaca gctccctctg ctataaggga ggtcgtctcc caaaggaaat
       61 caagatgcgt caaatcgtgt acagtcatat ttggcaaatc tgcagcaata cgacttacca
      121 agtgaccgac tctgtctcgc attgttaaaa attgaacacg aaaataatct tgttctgtga
      181 tatcggcatc aaggcgtgga tttacaaaca catgttgcca aagccatgtc tggcgaaaat
      241 cgagagaatt taacgccatt tctcaagtcc ccattttaat gttctaaact agaaaaatta
      301 taaaagacca cggactgtac ccatgtgaat ccacaatagc cattttcgcc aatttatgta
      361 tttcaaacaa gttttttatt agatttaatc ctttacccat cgaaggtccg caatttctat
      421 caaaggcccg tattcaaccc tccccctcaa tttcccttgt cagaaaaaat ctcaaatgag
      481 ttccagccat ccgtttttgt ttttggcggg aacggcatat ttttggccaa tgtatatccc
      541 tccgtgcgta tttacggtat ctttgccaat gcggttaggg gaaattcaaa gaaattaaat
      601 agagcccatt atcaaaacct tcaaaaattt tcaccctcca aacaggtttc acaaccgttc
      661 cttcagttcg accgaatcac tatcatagag actttcccct ttcaggaggc gttagagatc
      721 cacgtattct accggcctga tcctctccac caaaaatcga gtttcattat tgtccccatc
      781 cagagcgaac gagctaaatt tctgagagcg ggaagaaaga aaaacagttc tcccccctga
      841 cgactacaag accacaaagt gtataattaa gcgagatatt cccaacgacc attttgggac
      901 agagttcccc aaaggctatc taggaactaa ttgagacgta taacgaaaag ggtggtcaga
      961 cagaattcgt tcaaaaacga gaaaatgatg aagaaactat ttgcggtcta tgggcaactg
     1021 aaaagaagag tttttgtcct tctgatagtg agtctgtttt tgatagctcc ttttgcatta
     1081 gccggcccat cttccctcag aattatcctc ggcacattct ctccgtatta ttccccaaaa
     1141 tttgcccaca ttagaaccgg cactcttatc tcctgggaga accctaccgc cgaccttcat
     1201 tccatcactc acgatgggtg taagaccagt gaacgatgtg cctttgattc cggccctctt
     1261 ggccccaacc gcacattcac gcttcgtcat ttacctcccg gccagtatcc ctaccattgt
     1321 tcatttcatc ccatcatgca aggaaccctg gtggtgaccg ggcctgacac gtcaggtgag
     1381 acatgaaatg tttcagatga ggaccatcaa taaatctcaa agaatacccg ataagtatta
     1441 aatcggtttc aggattgccg acaagataac tgttacctca tatgagcagt cggatgagct
     1501 acacctacac aggaaagcaa aagtccaaaa accgaaaaac tacatctcat gactgcattt
     1561 attgtccaat gaagaaaccc gtcttctggc cgctgttgtt tttacttttt ctgttcagtc
     1621 ctttcatctt ttcttctgaa atttcgaaaa cttctcctgc cattcaaatt gaaggtcgcc
     1681 cccatgtttt gagtcaagcc atgcccccag atgacattga gagcaaagca cttccctcaa
     1741 agggtctgtt ccaggatcca acttctcaaa aataatacaa agcattttgg aaggaatgtg
     1801 gacagggaga cacaacgaaa cggttgcggc catttcacct gtaaccattc tggacggaga
     1861 ggctttgctt actgaggccc ccttctaccc ggggattcca ggtcatctcc aacggacaac
     1921 taggttcggg gtttgtctcg aaaggagccc agaaatacat tcgcacagag gaaaaaggct
     1981 cccaacaata gacctcctac ccccgtatag gcaaagaact ctgtcagaat atgcccgtga
     2041 gccggtcccc gaccaatgga cggcgccaac caaaacatcg tgtaccagga gagtggatag
     2101 aaactgctta gccccagaag gatggccgat gctttcttat aggtgggttt gagtgtggaa
     2161 aacatgacaa ggatgaccaa ggcaagcgaa aatgctgcaa tgcctgtggc atggaaatgg
     2221 gcccgttgag cgtaacgcca aattttgtca gcacttttgg aatcatgcac acccggatta
     2281 gccgtgatcc cttgcgcaat ataatccttg aacacatctt cattgacacc aaacacaata
     2341 cccattccca cgccaaacaa tagccctaac agcacgagtg caaggccgat cttaacgagg
     2401 ctcaactctt tttccatgtg tggtctccct gcactcaacg gctaggccaa tcggtgccct
     2461 ttgaattatt catcaaaatg gatttttatt tttttagctg catgacaaaa cgacggacat
     2521 gtatcttccc atgaggttca cacatcagca tacggtacgc caatgaaggt accatgaatg
     2581 ccctatgaaa gtttcttcat gaacgtcatc aaaggatagt atttctgtta ggtactctca
     2641 aggcaggata agccggaatt gaaccgctct catcgcttca ccttagcatg atgaatcgct
     2701 tccagtgccc ttgggcaacg ggttagaccc ttctccctca ggtttctaaa gccggcgtga
     2761 gatcttcacg ggtaacgggt ggttgtgtat acgacatccc gaccaggtta ctccatgggt
     2821 gggaatcatc ggcctgaaga tgcacccgcc gggaaggcat gataaaataa acaaacattt
     2881 aaaaggtccc ataaaggtct ccctgcttga acggttgttg ccatgttgag aaccctgaca
     2941 atgggagtca tctgcttgat gctgggtgcc tgttcgagcg ttgaaccggc aggaaagtta
     3001 cccacggcgt tgtcagttga cctctcccga tatagcggaa tgtggcatga gatagcacga
     3061 ctgccgatgt gggggcaacg aaattgcgta agatctacag ccgagtaccg gttactggaa
     3121 gcggggaagg ttgctgtgcg gaacgcatgt accacgtcga ccggtgagga gatcagcatt
     3181 gaaggggtgg ccacggtggt ggaccctgaa catcaggcaa agttgaatgt ggtcttcgac
     3241 cagtgggcag ctaaacttgt ggcgttgctt acctcatcgg agcaggggaa ttactggata
     3301 ctgcgggtcg atcccgacta ccggcttgcg atcgtcggca ctccggatcg tgactacctc
     3361 tggatcctgg cacgaacgcc gtcgctcgac gaggccactt accaggaggc agtcgccttt
     3421 agtcaccgtc ttggctttca aatagagcat ttgatccgtg ccccgatcag cgcagattaa
     3481 gttcttgcct cccccaatta aatatcaaaa tttttcgaaa gcggaattaa taagcggaag
     3541 aattttccta acgcggactt tttgcctttt caccatgcac ggaagcatcc cgccggttct
     3601 cgcggtttat gaaaaaaaag cctctagtat ttcctaacac agagaatcca ctcatgaccg
     3661 tttggactcg accttcctat tttgacaatg tataaaatcg gtctttcacg attttgccgt
     3721 gagaatattt atcaaaggtc tctcccatgg aatcgccgag ttctgccaat ctcgtgtcgg
     3781 gatgagggtg agtcttaaac agtaaggcga cgctgctgtc attgagaccc gcatggccaa
     3841 tttcctgcaa caccattggc aggccatagg cgtcatagcc ggctctggcg gccaacacca
     3901 ccccaattct gtcggcctga tattcggcat ctttatccaa tccgcgagcc atcacttctg
     3961 ctccactccc aataagatta ttcaccgcct gcttcgcttg ttcattcttc attttgccga
     4021 gtttttgcga aaacaagctg cttcccatat caatcgcctg gcttttctta atgatggtca
     4081 gatgatgctg tttgatgaca tgaccaatct catgtcccag gactccagcc aattccgctt
     4141 cagaatgaag ttggtgatac aaacccttgg tgagaaaaat aaatccaccc ggcgcagcaa
     4201 aggcattgat atcgtcggaa tcaatcactc caaaatacca atcaagatcg gttcttccac
     4261 tctgcagact tagccacttt ccaacttgat tgacataccg ttgcaagcgt tcatccttga
     4321 caaggggcgc agccccaagc agtcgtccgg caatctcttg accgatggct atttcctctt
     4381 ctgtggaagt ccctttgacc aggtcggtca ggtctgtcag tgaaggagtt ttcttgcccg
     4441 tctgtttctg cggagtaccg tcttgtgttt ttcctgtggt ggtttcatcc ggctgcccta
     4501 attgtttgag ggcatcaccc aaatcaaagg catgtgtgag ggttcccact ccggagaata
     4561 ccaatgtcag aatgcatgca cgcagaagga atcctttcaa acccgcactt gcctgttgat
     4621 tgtggaacac tagaggacca ggccgtaaaa attcaccgcg tatatttttc ttcatattat
     4681 gttgatggct catatgttaa ttcccgtctg tcggctgcat gaacggaact ttttgtactt
     4741 gtaatcctgc ctgattggca aattcttgtg cttgcttctt tgaagtccga taggttttga
     4801 gtgtctgtaa ttcttgctca ttaaactcgg cctccttcaa ttcctcttca tctaatccgc
     4861 gaactcccgt agcagccaca acatttccag tgccggctct cccagtggcc aggcccaaca
     4921 ctcctgaggc ttccgttaca gcggaaacct tttccgaagc gcccccccgc cgaatataaa
     4981 gcattcttac ccatccggtt ttcccttttc gggacatctc taaccatccg ccttttcttc
     5041 tcagaatttc cacggaatcc ccttccttta acgatagcac tactcgagca tcgttaaagg
     5101 gttcgctcat gacctggcac gttctaacgg ctacaccggg atccgatgct ttcccctctg
     5161 cgggaaagac tccaatcatg acgattgtca ggaaggccaa ccatttcatt atcacagtcc
     5221 tccttcttgc gttgtataga cacggcgatt ccgggtgcat ccctgaatga gtcgtgggat
     5281 tttcgggtaa aggatcatgc tgtaatctca agcggctgct ctttaagatt gaccatggct
     5341 ttaatcacta aatgttgcca agcctccagt ctggaatttt catttccata ggtcaatgga
     5401 ccttggcacc atccgagctt acatcctatt ctatcattct tacagatgct gcgaaccact
     5461 tctcgaacca gagataccgt ctgttcaccg gatttttcca cctcccatgg cttcgtttcc
     5521 acatgatttt cgacccaata aatgaggaac atattttgaa aggctttttc aatgccggca
     5581 ggtttccgtg agaagggttc tatcgtaaac ccaacctttt tgccatacag gccacgcttc
     5641 agcagggtca agaatcttcc ttccgggatt tcctccaacc tttccgg
//
LOCUS       DATIAL010000064         7873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1087223, whole genome shotgun sequence.
ACCESSION   DATIAL010000064 DATIAL010000000
VERSION     DATIAL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7873
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087223"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VLA60_02125"
     CDS             complement(<1..674)
                     /locus_tag="VLA60_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF08178.1"
                     /translation="MNWRLWLMVVGLISIFGCTESLPDYTLRFGLANSPTNLDPRYAT
                     DATSARLNRLLYSRLVDFGEGTIPIPALATWRELSPIHYRFELQEPRPLFHNGARLSA
                     NDVKATYASILNSATASPHRGILQIITRIDTPTENTVDFFLKQPDSLFPGYLVIGILP
                     ANLIAKGHPFHDHPIGSGPFQFLERPDDTRLVIERRADGQRVEFLRIPDPTVRTLKLL
                     ANEIHLL"
     gene            959..2329
                     /locus_tag="VLA60_02130"
     CDS             959..2329
                     /locus_tag="VLA60_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08179.1"
                     /translation="MSSAATMTSHGNATTQPLSARSLTAPTTIEETGLPEELLVQLLV
                     RTLYTQGELTERSAGEIMKLPYGVMRELFILIQREKLCEVKGHGESLGVLLRYVLTEA
                     GRQRATAFMDLCRYVGPAPVPLNQYINMIKSQPVNSLTIDRRTVTAATEHLVLTPGTV
                     DQLGEAVNSGWAIFLYGPPGNGKTVLAEAIGKMLEAVGGGEVYIPYAMEVDGQIIQVF
                     DPITHVKVEAPQEAARSLIEAKVEKDTRWVLCKRPIEFAGGELTMATLDLSFNASAKY
                     YKAPPHIKANGGVFLIDDFGRQLVRPRDLLNRWIVPLEKRVDYLTLHTGKVFQIPFDT
                     IVLFATNLEPAKLADEAFLRRIRNKIYIADPTPERFTQIFEEVCRKKGVPYDPEAVQY
                     LLDGVYVKYQLNMRSCHPRDLIEQIISIAKFNGVPPTLSKTFIDRACHTYFFVEAAEY
                     DQNTSQ"
     gene            2493..3350
                     /gene="purU"
                     /locus_tag="VLA60_02135"
     CDS             2493..3350
                     /gene="purU"
                     /locus_tag="VLA60_02135"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HSF08180.1"
                     /translation="MSNATAILLITCKDHKGLVARVSGFIHEFGGNILDSDHHTDRET
                     GDFLMRTVFDLAGFQIPQEEIANAFGPIAKIFNMNFQIYFSDRKPRIGILVSQYDHCL
                     VDVLQRHRRGELAIDIPVVISNHQTTEWWADLFHIPFRYVPVTPQTKEQQEREVLSVF
                     REHKVELVVMARYMQIVSEKFLQEIRCPVMNIHHSFLPAFVGAKPYHQAYDRGVKIIG
                     ATAHYATPELDEGPIIEQDVVRVGHRETVQDLIRKGRDLEVMVLARAIRLHVEHRVLV
                     YGRKTVVFD"
     gene            3813..4847
                     /locus_tag="VLA60_02140"
     CDS             3813..4847
                     /locus_tag="VLA60_02140"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSF08181.1"
                     /translation="MKQIWLVLVLFFVVGNVGTLLAQDLLLIVDPEAKFEQVFSGGFW
                     LESPAVAPDGSVYFSDIPHTTVSGMQAGRIWRYDPRTGVTTIFRSPSGMANGIVFDAR
                     GDMIVAEGADFGGRRITHTEMNTGKSNIVAGLYNGNRFNSPNDLTIDTRGRIYFTDPR
                     YTGYEPIEQSVMGIYRIDPDGSVHLLAANLWQPNGLAVSPDQKTLYVVEVGTYNTNIV
                     EKPPIVGGPPSAVHAYDLFDDGSLRYRSMVVTFPTGTWGDGMTVDGEGNIYVGVGGPP
                     GSNGVHIYTPSGQQIAFLETEETAVNLDFGRGADANLLYVVCARLPSGSGPWTPPSSN
                     GLYRIRLQME"
     gene            4963..5436
                     /locus_tag="VLA60_02145"
     CDS             4963..5436
                     /locus_tag="VLA60_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HSF08182.1"
                     /translation="MKIGVGADPFGLELKEAVKRHVLTLGHVCVDVGGTTDEARPYYE
                     VAHELAGKVGSGECDRGILVCGTGMGMAIIANKHPNVYAAVCEDPSTATKARSINNAN
                     VLTMGGMVTASYKAKEIVDAFLTTEFKSGWDEEIQSFLDRSMVDIQRIESEEFKY"
     gene            6142..7257
                     /locus_tag="VLA60_02150"
     CDS             6142..7257
                     /locus_tag="VLA60_02150"
                     /inference="COORDINATES: protein motif:HMM:NF044863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08183.1"
                     /translation="MKLPVLAALHRFVDTVFLSSLRKEEGKSVNFSAVLVGRQEGIES
                     VSINRSPLFVLEETVPFTSSILPKFAAAFDPKLSTIAVESNEADGNLYIWGVFQHAQK
                     TNILSEVSVVIPENSSMRPDFFTLSTRGRGSFAVSRGRALIGRFQAGYFVAASPTPFM
                     SNSLGAHVLAAIKRDPLYTKYNAKYWHFIRPLLDLLLEEISLRGHGATIVILEADRPS
                     IANHYVPKHELVGKYNLKDTIEKCILNGSNTVMGIAYRKFACEIIQRIAQLATVDGEL
                     VIDTLFNVLAFGAILIAPDSEHPALIGPDGFGNPNGQPFDISRYGTRHRSAFNYSAAN
                     HNSTVFVISQDGPIRAFRGDGDRSVLVWPDCNTSMYV"
BASE COUNT         1925 a         1888 c         2072 g         1988 t
ORIGIN      
        1 aggagatgaa tctcatttgc caacagcttg agggttcgaa cggtcggatc gggaattctt
       61 aaaaattcaa cacgttgacc atctgctcgt cgctcgataa caagccgggt gtcatcgggg
      121 cgttcgagaa attgaaaggg cccgctgcct atggggtggt catggaaggg gtgccccttg
      181 gcaataaggt ttgcggggag aatgccgata actaaatagc cgggaaataa tgaatcgggc
      241 tgcttcagaa agaaatcgac ggtattctcc gtcggcgtat cgatccgggt gataatttgc
      301 aaaataccgc gatgtggaga tgccgtggca gaattcagaa tggaggcata ggtggctttg
      361 acatcgttag cggagagtcg tgccccattg tggaagagcg gcctcggttc ttgtaattcg
      421 aatcgataat ggatgggaga gagttcacgc cacgtggcca aagctggaat cgggatcgtg
      481 ccctcgccga agtccactag gcgcgaatac agcagccgat tgagtcgagc cgaagtggca
      541 tccgtggcat aacgcggatc taaattcgtc ggcgaattgg ctaacccaaa acgcaacgtg
      601 taatcgggaa gcgattcggt gcagccaaaa attgaaatga ggcccacaac catcagccat
      661 aatcgccagt tcacctcaaa gccatgaagc attcttttgg caaaaccgat tggggaacac
      721 acaggggatc gattaggtct gagattgcga tgttcttgga aagaaatcct catgcaacct
      781 taggaaaacg gcgtatgaac gagacgggga aatagcgtgg ggatttatgc atggcgatgt
      841 ttttctaatt ttacagggat tatccctatt gtaaaagggc gaaagttgac gaatcgaaga
      901 acctctgtct tgccatgtcc ccttattcaa gaaatagact aatcaaagga gattacgaat
      961 gagttcggct gccacgatga catctcatgg aaatgcgacg acccaacctc tcagcgcccg
     1021 ctccttaacg gctccgacca cgattgaaga gaccggattg ccggaggaat tgcttgttca
     1081 gcttctggtc agaaccctct acacccaagg agaactcacc gagcgatcgg ccggagagat
     1141 tatgaaattg ccttacgggg tcatgaggga gctgttcatc cttatccaac gggagaagtt
     1201 gtgcgaggtc aagggacatg gggagtcgct tggggtgtta ctccggtatg tgttgacgga
     1261 agcgggtcgt cagcgagcca cggcttttat ggatctgtgc cggtatgtgg gaccggcccc
     1321 ggttcccctc aatcagtata tcaacatgat caaaagtcag cccgtcaata gtttaaccat
     1381 cgaccgacga accgtgacag cggccacgga acatctggtt ttgactcctg gcaccgtgga
     1441 ccaactcggg gaagccgtca attccggctg ggccatcttt ttgtacgggc ctcctggaaa
     1501 cgggaaaacg gtcctggcag aagccattgg gaagatgttg gaagcggtag gcgggggaga
     1561 agtctatatc ccctatgcca tggaggttga tggacaaatc atccaagtgt tcgatccgat
     1621 tacccacgtc aaagttgagg ctccgcaaga agccgccaga tccttaattg aagccaaagt
     1681 ggaaaaagat acccgctggg tgctgtgtaa gcgtccgatc gagtttgccg gcggtgagtt
     1741 gacgatggcc acgctggatc tctcgtttaa tgcgtccgcg aaatattaca aagcacctcc
     1801 tcatattaaa gccaatggcg gggtgtttct gattgacgac ttcggacgac aattggtccg
     1861 tccgcgagat ttgctcaacc gatggattgt gccgttggaa aagcgggtgg attatctgac
     1921 cttgcatacg ggtaaggtgt tccaaattcc attcgatacg attgtcttgt ttgccacgaa
     1981 tctggaaccg gccaagctgg ccgatgaagc gttccttcgc cggatcagaa ataaaatcta
     2041 cattgccgac ccgacccccg aacggttcac acagattttt gaagaagtct gcaggaaaaa
     2101 gggggtgccg tatgatcctg aagccgtgca gtatttattg gatggtgtgt atgtaaaata
     2161 tcaattgaat atgcggagtt gccatccccg ggatttgatc gagcagatta tcagtattgc
     2221 aaaatttaac ggggtgcccc cgaccctctc caaaacgttt atcgatcggg cttgccatac
     2281 ctatttcttt gtagaggccg ccgaatacga tcagaacacg agtcagtaac gtttacccta
     2341 ccgatgcctt tcgctatgat ggcccaactt tccagggagg gagtgttggg ccatcgtttt
     2401 ttctgttctc agacgttcac gattgctgtg tgagcgtgct gcctcatctc tatgtcttgg
     2461 aggtctctgt ataccatcct gaaggagtcc ctatgtccaa tgccacggcc atcttattga
     2521 ttacctgtaa agaccataaa ggccttgtcg cgagggtgtc aggattcatc catgaatttg
     2581 gaggcaatat tctggattcc gatcaccata cggatcggga aaccggagac tttcttatgc
     2641 ggaccgtgtt tgatttggcc ggatttcaga ttcctcaaga ggagattgcc aatgcctttg
     2701 gtcccatcgc caaaattttt aacatgaatt ttcaaatata tttttcggac cggaagcccc
     2761 gaatcggcat tctggtttcc caatacgacc attgtctggt cgatgtgttg caacgacacc
     2821 ggcgagggga gctggcgatt gatattccgg tggtgatctc taatcaccag accacggagt
     2881 ggtgggcgga tctctttcat attccctttc ggtacgtccc tgtcacgcca caaaccaagg
     2941 aacagcagga gcgggaggtt ctgtccgtat ttcgtgagca taaggtggaa ttggttgtga
     3001 tggctcgcta catgcaaatt gtgagtgaga aatttttaca ggaaatccga tgccccgtca
     3061 tgaatatcca tcattccttt ctgccggcat ttgtgggtgc caagccctat catcaagcct
     3121 atgaccgtgg agtcaaaatc atcggagcaa ccgcgcatta cgccacaccc gaattggatg
     3181 aaggtcccat catcgaacag gacgtggtgc gtgtcggtca ccgggaaacc gtgcaggacc
     3241 tcattcgcaa aggccgtgat ctggaagtca tggtcctggc cagagcgatt cgactccatg
     3301 ttgagcatcg ggtgttggtc tacggccgca aaaccgtcgt gttcgattag ggggagtgtt
     3361 gaaagcggcc gccagcggag ttctcgccgc catgccgtgc tcacgtactc ctccgtacgc
     3421 tccgcccgcc caagcggctg cggcctggct ggacgagcct ttttgaacac tccctcattt
     3481 tcccctgagg atctcggtaa cccatgaatt ggtgaaaagg caaaatattt gaagctttca
     3541 acagtccctt aataggatgt tgaaggagtt cgccctgagc cgagtcaaaa gtgtctccaa
     3601 cagttaagaa tgttctgacc gtttgaattt cccttttccc ttcgcagacc tccaacttga
     3661 catattttgc cgtcttcaag agaatcgccg gcatgcgaca ggcttgaaat ggaggctccg
     3721 catctcatgc ggattcagat gatttttttg ataagtcctt catgcaaagt agccggtcac
     3781 ttggcatttt tcgaaagaaa gtgaacatca cggtgaaaca aatctggcta gtcctagtac
     3841 tgttttttgt cgtgggcaac gtggggacac tcctcgctca ggatttattg ctcatcgtgg
     3901 accccgaagc caagttcgag caggtgttca gcggtggatt ctggcttgag agtccggctg
     3961 tcgctccgga cggctctgtt tacttctcag acattcccca cacaactgtg agcgggatgc
     4021 aggccgggcg tatctggcgg tatgacccac gcacgggcgt gacgaccatc ttccgctcac
     4081 cgagtgggat ggcgaatggc atcgtgtttg atgcccgtgg cgatatgatt gtggccgaag
     4141 gtgccgactt cggtggccgt aggattacgc ataccgagat gaatacgggc aagagcaata
     4201 ttgttgccgg actttacaac gggaatcggt tcaactcgcc aaacgacctg accattgata
     4261 ctcgcggtag aatctacttc accgatccgc gatacacagg atatgagccc atcgaacaat
     4321 cggtgatggg catctatcgg attgaccctg acggcagcgt gcatcttctt gcggcgaatc
     4381 tgtggcagcc aaacggactc gccgtctcgc ccgaccagaa gaccctctac gtcgttgagg
     4441 ttggaaccta taataccaat atcgtggaga agcctccgat agttggcggt ccaccgagcg
     4501 cagttcacgc gtatgatctc ttcgacgacg gctctcttcg ctatcgaagc atggtggtga
     4561 catttcccac ggggacctgg ggtgatggga tgactgtgga tggggagggg aacatctacg
     4621 tgggagtcgg tggccctcct ggtagcaatg gagttcacat ctacacaccg agtgggcaac
     4681 aaatcgcatt ccttgagaca gaggagacgg ccgtgaatct cgactttggg cggggtgccg
     4741 acgccaatct tctgtatgta gtgtgcgccc ggcttccttc tggttcgggt ccgtggactc
     4801 caccctccag taacggcctg taccgcatcc ggcttcaaat ggagtagatt gcacgcagaa
     4861 tttttgtgag gtgtttggag tcaagcttaa ttgtggacta tagtctcatg tgcatatgta
     4921 gggtgaggtg ccgatggtgg aaactgcaag gagagactgg acatgaaaat tggagtcgga
     4981 gcagatccct ttggcctgga actgaaagag gccgtgaagc gacatgtcct gacactcggg
     5041 catgtgtgcg tggatgtggg cgggacaacg gacgaagcac gtccctatta tgaagtcgct
     5101 catgaactgg ccgggaaagt gggttcaggt gagtgtgatc gcgggatcct ggtgtgcggt
     5161 acagggatgg gcatggcgat cattgcgaac aagcatccga atgtctatgc cgcggtgtgc
     5221 gaagatcctt ccactgcaac caaagcccga tccatcaaca atgccaacgt actcaccatg
     5281 ggtggcatgg ttacagcatc ttacaaagcc aaggagatcg ttgatgcgtt tctgacgact
     5341 gaattcaaga gcgggtggga tgaggagatc caatcttttc ttgaccgttc gatggtcgat
     5401 atccagcgga ttgagtccga ggaattcaag tattgaattc agataaaggg gacagtgcgt
     5461 gaataaatgc ttttgccaat gaatcgcttc gatgcggacg caatcctgca cgtacgtctg
     5521 caggtgaaac gcacacgcaa attagatccc attcggaagg gacgtcatct tgatggaatg
     5581 gtttgacgga ctggtcttgg cctgagcgat ccgactgcat attgagcatg aggtgttagt
     5641 ctacggacga aaaaccgtcc tgttcgatta gcgtgttaaa aaaaatgccg ccagcagagt
     5701 tctccctacc tggctctctt cgggcgcaca gacttgacgc tcttcgcgtt gtgcgcactc
     5761 acgtactcct tcgtaggctc cgcccggcca gaggactgcg gccttgctgg accagttttt
     5821 tgagcactcc catatttgtc cgttttacta tcgggttttt gcaagtggcc aatcggtgaa
     5881 gggtcacttg aaataaggcc atttgaacag caatttattg aaattccaaa agttatcaga
     5941 tacttttcca gaaggagtaa tcggcgttat aaaaggtagg taatcgagga ttcacatttt
     6001 cctttggctc catctagaca tttttaaatc ccaggtatat cggctcatat gattaatcca
     6061 gtacttagcc ctgcaatact ccagaacatc tgtaccgcat ggagcgcaga ccaggcccat
     6121 ccgcttcgcc gtcggaggaa tgtgaaattg cctgtcctcg cagcactgca tcgctttgtg
     6181 gacactgttt tcctttccag tcttaggaaa gaggagggca agtcagtcaa tttttccgca
     6241 gtcctggtgg gaagacaaga gggtatcgag tctgtatcga tcaaccgttc cccacttttt
     6301 gttctagaag aaacagtgcc ctttacgagc tcaatactac ccaagtttgc tgccgccttc
     6361 gatccgaagc tctcgacgat agccgtagag tctaacgagg ctgatggcaa tctttatatc
     6421 tggggtgttt ttcagcatgc tcaaaaaacc aacatccttt ctgaagtctc cgttgttatc
     6481 ccagaaaatt ccagcatgcg tcctgacttc tttacccttt caaccagagg tcgcggctct
     6541 tttgctgtct cgcggggtag ggctctaatt ggtaggtttc aagccggata cttcgtggcg
     6601 gcaagtccga caccttttat gtccaattcg cttggtgctc acgtgcttgc agccataaag
     6661 cgtgaccccc tgtacacaaa atataacgcg aaatattggc atttcattcg tcccctactt
     6721 gatttattgt tggaagaaat atcactacga ggccatggag ccacgattgt tattctcgaa
     6781 gcagaccgtc catcaattgc aaaccattat gtgcctaaac atgaacttgt cggtaaatat
     6841 aatctcaaag atacgattga gaagtgcatc ctgaatggca gtaataccgt aatgggaatc
     6901 gcatatcgta aatttgcttg cgaaattatc cagcgaatcg cacagttggc aaccgtagat
     6961 ggagaacttg tcattgatac tttatttaac gttcttgctt ttggtgcaat ccttatcgca
     7021 cctgacagcg aacaccctgc gctaatagga ccagatggat ttggaaaccc taatggacag
     7081 ccttttgata tttcacgcta tgggactcga catcgatcgg ctttcaatta ctccgcggca
     7141 aatcataatt caacagtatt cgtaatctcc caagatggac ctattcgcgc gtttcgtgga
     7201 gatggagacc gtagtgtttt ggtttggcca gattgcaaca cctcaatgta cgtataggaa
     7261 agtttaaggg gcacgggaaa gagacctgga gtcgtttttt gacgggtcaa taggagttgg
     7321 tgcccaaagg attaagccac gtcgtccgcg cctgggcact ggcgtcctgg cttataaggt
     7381 gctcaatcgc tgggtggggc ggctctccct ttttaacaac cctgccgact atgccggatt
     7441 tgaaaacgtt ttggcggaat ttcaccggcg gatcgggaaa catttccccg cctattgttt
     7501 aatgcccaca agctggcacc ctatctggtc tcgcagggat aggtcatcgc aaggcttttg
     7561 ataattccct tttccttttt ccatttcttc gtcttgacat gtttcgtaaa ttgacgaaga
     7621 atccggactc gattattcca gattccccaa taggtgccaa ccaggaatat aataattggc
     7681 agatggaaaa taccttctta aggctaaaga acagggtaag cgcacaaaaa gaaatggtaa
     7741 cctttcatcc agaaaaatac agcctgtaaa tgcctttatt cagcctttat aaacatcctt
     7801 agatactcgt gaaacagcga gcatcatttc aacgacgcgt tgttgcatga accgcatatt
     7861 aactacttac atc
//
LOCUS       DATIAL010000065         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1194894, whole genome shotgun sequence.
ACCESSION   DATIAL010000065 DATIAL010000000
VERSION     DATIAL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194894"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            434..760
                     /locus_tag="VLA60_02155"
     CDS             434..760
                     /locus_tag="VLA60_02155"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HSF08184.1"
                     /translation="MSHIGKYVIMCGVSMLMAGCQEPPIHELQEARQAVEKAKQERAY
                     IYAPDLYNLAESELTIGEEEIHDQSRKIFWSRDYSVATRLMNLAQTDAHKALSLAQEE
                     KQKASL"
     gene            1142..2005
                     /locus_tag="VLA60_02160"
     CDS             1142..2005
                     /locus_tag="VLA60_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF08185.1"
                     /translation="MEQLIDSPGNMLIRYGLSTFFLVLIIIIIRMGIHHSLFRSTELS
                     VETQRRWAVNLRNGLLFLFILGMIFIWAPQLQTFAVSVFAVAVAFVLATKEIIDCLSG
                     SGLRMLTKAYSLGDRIEIGGIRGNVVDHNALTTTVLEIGPGQTSHQYTGRAMVIPNSF
                     IFDHPLTNETYTRKFRLHIVTVPLSTDDDWRTAERLLLQAGEEESAPFIEEARTYLKR
                     LEGKLWLDAPSVEPRVTIQLPEPGRINLLLRVPCPTQYPSRLEQAILRKFLSEFSFVP
                     RLFTQESHSSH"
BASE COUNT          528 a          583 c          460 g          550 t
ORIGIN      
        1 ggaacggaga ctatctcatt tttcgccctt ggatgtcaga tgtaccggcg aggtcactct
       61 tcattgttcc tcgaaacaat ggatctacaa ttcataatga aaaatggggc cacctctctc
      121 taataaagaa gacctgtcaa ctttgaggtc ggatcatgaa gtcatgccta acgagaatgt
      181 cacagccctc agaccgcatc gagacaaaca ttccaattct gaaccaacct gtcctcccac
      241 tccaagcccg gctttaataa aacttcagaa aattccgtta tctccagctc tttaacatca
      301 ccctctattg gcgtaggttt tgcttcatga gctttcaggt agcgatgaca gcagccatta
      361 gagttcccca aaaaagttcg atgaattact aagtgaatta tttagggaaa tatcggacgt
      421 aaggattcag caaatgtccc acatagggaa gtatgtcatc atgtgcggag tgagcatgct
      481 gatggccggc tgtcaggaac ctcccatcca cgaattgcag gaagcacggc aagcggtgga
      541 aaaggcaaaa caagaaagag cctatattta tgcccccgat ctttataatc tcgccgagag
      601 tgaactgact attggtgaag aagaaattca tgatcaatcc cggaagatct tctggagccg
      661 cgattactcc gtggctaccc gattgatgaa tctggctcaa accgatgctc ataaggcctt
      721 gtctttagct caagaagaaa aacagaaagc gtccctgtag gtcagatacc gtccgcatcg
      781 cggtcaattc cgacataatt cttcattgcc tggaatcacc ttcgcacctc tggctttccc
      841 acgagagagc attcttgcct ctcggcaacc acatcccgtc cgcccccttt gattaacaga
      901 gaaaatacag tatgatcgaa gaaaggttct ggtctcaacg ttatccacat acggctgcca
      961 ttgtgagttc gtcttcaagc tgatctgttg tgattcaccc gttattgatc gttcatcttc
     1021 gattcttcgt catccgtaac tttcaagcgc ccaggtcggg actgatgcct gatgctctgc
     1081 caatggtggg catttccatt ttgactcatc ccgatcatcc catcgttatg aggagcctca
     1141 cgtggaacag ttaatagatt ctcccggcaa tatgcttatc cggtatggcc tgagcacctt
     1201 tttcctggtg cttatcatca tcatcatccg gatgggcatc catcacagcc tctttcgctc
     1261 aacagaactc tccgtggaaa cgcaacgacg gtgggcggtc aaccttagaa atggactgct
     1321 ctttctgttt atcctcggga tgattttcat atgggccccg cagctccaaa ctttcgccgt
     1381 ctcggtcttt gccgtcgcgg tcgcgttcgt cctggccacc aaggaaatca tcgattgctt
     1441 gagcggatca gggctccgga tgctgaccaa agcctattca ttgggtgatc ggatcgaaat
     1501 cgggggcatc cggggaaatg ttgtcgatca caatgccctg actacgactg tcctggaaat
     1561 cgggcctggc caaacttccc atcaatatac gggacgggcc atggtcatac ccaatagctt
     1621 tatttttgat catcccctca ccaatgaaac ctatacacga aaatttcgtc ttcatattgt
     1681 gacggtccct ctgagcaccg acgacgactg gcgaaccgcc gaacgactgc tgctgcaagc
     1741 cggagaagaa gaatccgccc cctttatcga ggaggcccgg acctacttga aacgactcga
     1801 aggaaagctg tggttggacg ccccttccgt tgaaccccgt gtcaccattc aattgccgga
     1861 acccggccgc atcaacctct tactccgcgt gccctgcccc actcaatatc cttcccgact
     1921 ggaacaggcg atccttcgga aatttctgtc cgaattttcg ttcgttccgc gtctatttac
     1981 acaggaatct cattcttccc attaagactc gattggcctt gccccatctc tctatggtaa
     2041 acattagaaa ttcgttttga catttcagca tcctcgctga aacagatatc catccaacac
     2101 atggtcggtt tcaggactcc t
//
LOCUS       DATIAL010000066        10628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_657207, whole genome shotgun sequence.
ACCESSION   DATIAL010000066 DATIAL010000000
VERSION     DATIAL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657207"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VLA60_02165"
     CDS             <1..993
                     /locus_tag="VLA60_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08186.1"
                     /translation="FMFLRFSSAFRVAWCGIGIFVMVECNHHAVAAPRSPYDPEFVPF
                     TVKIKDEVNPYRVLGIYVMPSGTIDLECVFTNPASSFTVSAGGGNLERHAKERWKWKA
                     PNQKGLYLITITDTTTHATMVLNVFVMVPFAHHGEKLNGYRIGRYQDQPLKNNPVYNR
                     PQGFVEVTQNNRNVLVSPHFTLGQFVGKQPSGYPKYLLLRERLLLKLEMILEAVNQEG
                     IKTHTLHIMSGFRTPFYNHSIGNRTKYSRHLYGGAADIFVDVDGNNQMDDINQDGEIT
                     IADAVVLADVVKKKSRETWYQPFLGGLGIYGPKPHRGPFVHVDVRGFKARWMKP"
     gene            1225..2220
                     /locus_tag="VLA60_02170"
     CDS             1225..2220
                     /locus_tag="VLA60_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08187.1"
                     /translation="MALFFMSTAVIGLVGWKLTQLVDRLADRTGIGEALAGAVFLGAS
                     TSLPGIVTSVTTAWGGHAEFAVSHALGGIAIQTVFLAIADMTYRQANLEHAAASSANL
                     MNGVLLMSMLSLILLAIVGPEFSMWNVHPVTPCVVAIYIFGLRLVHHSHVNPLWSPKI
                     TSETKADEPEAGNCHESLQKLMMGFGASALLIGTAGWILAGSAVSLVTHTGFSETLVG
                     GVFTTMSTSLPELITSLAAVRRGALTLAVGGIIGGNAFDTLLVAFSDLAYQNGSIYHA
                     TTNQQVYLIALTMLMNGILLLGLLRREEHGIANIGFESFFILLVYLGGITILFLA"
     gene            complement(2285..3634)
                     /locus_tag="VLA60_02175"
     CDS             complement(2285..3634)
                     /locus_tag="VLA60_02175"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF08188.1"
                     /translation="MILAIEDRLARDEFVQENLIDVVSEKGIVTLLGEVDNILAKERA
                     AALTGTIKGVRSVINQILVNPSSRHEDQEIEDQVKSALLENPATELYDMNVHVTLGRV
                     TLTGTVQSWQEKQLALQVAKGVEGIKDIQDKLTIMTVKNRSDNAIKQEILQRLNHDVW
                     INPSSVRIAVRDGQVTVFGTVRSVDEKSRILSLSWVQGITSVNGEQLKVEWSDREPMR
                     RSGFHIPSDETIQDAIYDALSYDPRITNADITIEVKEGLVTLTGIVDNLQAKTTAEED
                     TWNTTGVYNVVNLLKVRMNPKYSDEDIAARVKAVFLRDPLLDHKDINVKVYNQAISLK
                     GTVKSRSQKSRAGLLASNVKGAVTVNNRLEYATEWQDKSDPELRQDIKDQLWWSPFLS
                     DHSISVHVEDGVATLMGFVPTWQQRYLAEEHAFVAGASFVHNRLKVDPSSWTPHSTQ"
     gene            complement(3868..4590)
                     /gene="smbP"
                     /locus_tag="VLA60_02180"
     CDS             complement(3868..4590)
                     /gene="smbP"
                     /locus_tag="VLA60_02180"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HSF08189.1"
                     /translation="MQQNFSRKSFITLISMTLCIFSFLDMSFGKTRSFEQTPIPPIKL
                     VKDVQENPEPYIKDVHQQLQKSRALLNDLKKMRTQASSQKKQEDLYAPIMKLTKELEH
                     AERISKNLDVIDPQPWLRNKTEMDAVLADLEVTYDETLPLLQDRTRFLQDAVHHAKIA
                     MEYGGEGRKDLFHKNALAAKKAASLAQKEGVDSAFVKEGISELEDALSHIKQDRIAAA
                     ATLTEGAYLHLNSALKKSQNSE"
     gene            complement(4647..6107)
                     /locus_tag="VLA60_02185"
     CDS             complement(4647..6107)
                     /locus_tag="VLA60_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF08190.1"
                     /translation="MRNIFRQSLISLFLWVMISLVTLPSIGLAEVDQNVITDKDITVA
                     IESRFLVDQMVPSNGIDVHTDNGVVMLSGEVPTLLARERAGKIVASIRGVQALINTIA
                     VNPTRRIGDKELRFRVFTALAADPASDSYEITVQVDQGRVMLTGTVESWQEKHLTEEV
                     VKSVKGVQSLRSRITVNPKASRPDSEIEAEIFRRLQSDVWVHESLIGIMVDQGHVTLT
                     GTVGSLAEKNSAYTDAWVGGVKGVNVAQLNVEWWARDKMLRHRKDAFTSTTHTAEAIR
                     TAFTYEPRLQDSDIDVRVVEGTAFLIGIVDNLAAKYAAEETTRNTEGIWRVRSFIKVR
                     PPVRLPDRDLEKRVREAFNQHPLINGYEIKISANSGKVSLEGYLNSPTEISQTVRAAA
                     RVKGVIDVVNYLQIQSPGKLDEEIWEEIRRAFWWDPGLFDQDITVTVSNGTVTLKGTV
                     PSIVEWRRAREVAQDSGAERVRNRLRVQYGPDFYST"
     gene            complement(6508..6981)
                     /locus_tag="VLA60_02190"
     CDS             complement(6508..6981)
                     /locus_tag="VLA60_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HSF08191.1"
                     /translation="MLTIFLNILFFSALILSLSHAEEIGSVDTKFNFLGPDHKIVIEA
                     FDDPKIEGITCHLSRAKTGGLKGMVGVAEDTSDASIACRQVGPIRIIDELEEGEKVFH
                     ESRSLIFKKLQVVRFFDKKRQTFIYLVYSDKVIEGSPKNSISTVPIRSWSNPQQR"
     gene            complement(7168..7380)
                     /locus_tag="VLA60_02195"
     CDS             complement(7168..7380)
                     /locus_tag="VLA60_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSF08192.1"
                     /translation="MGVGVGLAMIAVVVVSNLLGVLFPVLLTKVGINPAVASSPMVTS
                     TTDVTSLILYFFIVRLVIDSLQGGGL"
     gene            complement(7919..9280)
                     /locus_tag="VLA60_02200"
     CDS             complement(7919..9280)
                     /locus_tag="VLA60_02200"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSF08193.1"
                     /translation="MTLEPLETSMTVGRVAIAASALTHTLFATFIVGSSLIGAVTATF
                     SYVTEEEWFHRLAKLIAFALVLSTATISFFGVVLVFALNIYWPQFWHRLFHVMFWPFL
                     AEAALFLGEAIFAYAWYYLWEWGRHGLRQKVHLAFIWLAAGCALSAMFMIDITASYML
                     TPDPIHSAWENVFNPTMIHLDLHRWFGNLTWSGFALGGICAVRYLKSTIDTDRNFFHK
                     GGSYCFLIGYGALLVMPVVGYQYLLHLRYGQPQAFYTVMLGERSWLFDVVALLYGLLI
                     LVGFLYVMQMVRSQSKPPPSFIGFIPLSLTVIVIAAMILAMPYQLQHIPLLNRLTDQE
                     INSFGKMQPYKYFALSSLVIFGFAHWLYSFRVFGRMPQATVSRAVSGARRRMAGLLIT
                     LAGLTVIILLAMGWVRESARAVNGYLIYDEMRLEDEESTYQASPSEKGIFSQEIPYDS
                     SER"
     gene            complement(9277..10452)
                     /locus_tag="VLA60_02205"
     CDS             complement(9277..10452)
                     /locus_tag="VLA60_02205"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSF08194.1"
                     /translation="MMTSSSFWIGFFSLAHITLASLGVGFMVLAPIAEGLGRSRPYFT
                     DFAYLATRFTLVTYTTSIVLAVFMLELFIGLFPLTNSYLFNHFRYPLHLALIVFFIQV
                     LCLYPYYHFWDRLRSKSITWHITLGTVAAVLILIWGGILDGIGSYMMTPVEGDSGWAR
                     LWNPTWVPLMVHRFFGNLVIAGYVMAAYAGWRLRKQSGQKKDDAYYLTLIKTGMAIGI
                     VSLMIQPVSGFVFSQHIHNAQPKILQDLYEGQTVLLVIWQFTLLALLLLGSHLIFRSV
                     HLERGHSFRGEVFYVLTVVLMVVLAPYPWYRRIVNLIVLLQTGWYFLYVFPVWMRGST
                     PSLNKPFIHFIAMALGLAALVLYLTMGTIREVARGPDTIHGIINCQTESTFRGETFP"
BASE COUNT         2793 a         2563 c         2481 g         2791 t
ORIGIN      
        1 ttcatgtttt tgcggttcag cagtgctttc agggtcgcat ggtgcggaat aggaattttt
       61 gtgatggtgg aatgtaatca ccatgctgtc gccgcacccc gttcacccta tgatccggaa
      121 ttcgtgcctt ttactgtcaa aataaaggac gaagtcaacc cgtatcgggt tttaggcatc
      181 tatgtcatgc cctccggaac aattgacctt gaatgcgtat ttaccaatcc tgccagctct
      241 tttaccgtct ctgctggggg gggcaacctt gaacgtcacg caaaagagcg atggaaatgg
      301 aaggctccca accagaaagg attatatctt ataacaatta cggatacgac cacccatgcc
      361 accatggtgc tgaatgtgtt tgttatggtg ccgtttgctc atcacgggga aaagttgaac
      421 ggttatcgga tcggtcgcta tcaagatcaa ccattaaaga ataatcctgt gtacaatcga
      481 ccccagggct ttgttgaggt cacccagaat aatcgcaatg tgcttgtctc gccgcatttt
      541 acgcttggcc aatttgtcgg caaacagccg agcgggtatc ccaagtatct gctcttaagg
      601 gagcgactat tactcaaatt ggaaatgatt ctggaagcgg tcaatcaaga agggattaaa
      661 acgcacactc tacatatcat gagtggcttt cgcacgccat tttataatca ttcgatcggc
      721 aaccgcacaa aatacagccg ccatctgtat ggaggtgcag ccgatatttt tgtggatgtg
      781 gatggaaata atcagatgga tgatataaat caagacggtg aaattaccat agcggatgcc
      841 gtcgtactgg cagatgtcgt gaagaagaaa tctcgtgaaa cctggtatca accttttctg
      901 ggaggattag gcatttatgg gcccaaacct catcgaggtc catttgttca tgttgatgtt
      961 cggggattca aagctcgatg gatgaagcct taaggtcata ctgcatccct cacttctcac
     1021 aaaagcaaaa cacatgttca ttgatggcat catgtccagt gacaatgggg aggtttactt
     1081 ccgggccctt tctcaggttt caaatgttaa acatttaaga accataagct tctgccctgt
     1141 aaacgataat cgggtcaatt acaagcatag aagcggatag gatcttttat cctggaacat
     1201 gaaggttggt ctctcactct tagtctggct ctttttttta tgtccacagc cgtcatcgga
     1261 cttgtcgggt ggaaacttac tcaattggta gaccggctgg ctgatcgaac aggaattggg
     1321 gaggcgcttg cgggcgccgt tttcctgggg gccagtacat cattgccggg tattgtgact
     1381 tccgtgacga ccgcatgggg cggtcatgcc gaatttgcgg tgagccatgc cctgggaggc
     1441 attgccatac aaacggtttt tcttgccatt gccgacatga cctatcggca ggccaatctg
     1501 gagcatgccg cagcgtcctc cgccaatctc atgaatgggg tgctcctcat gagcatgctc
     1561 tcgctcattt tactggccat cgtgggtcct gaattcagca tgtggaatgt gcatcccgtc
     1621 actccatgtg tcgtcgccat atacattttt ggtcttcggc tggtacatca ttcccatgtc
     1681 aatccacttt ggagtccgaa aataacatcc gaaactaaag ctgatgaacc ggaggcgggc
     1741 aactgtcatg aaagtcttca gaagttaatg atgggtttcg gcgcttctgc cttgctcatt
     1801 ggaacggccg ggtggattct tgccggttca gccgtatctc tcgtgactca taccggattt
     1861 tctgaaactc tggttggagg agtctttacg accatgagta cctccctacc tgaattgatt
     1921 acctcacttg ccgcggtgcg taggggggcg cttacgcttg ccgtaggagg aattatagga
     1981 ggaaatgcct ttgatacgct tcttgttgcg ttttccgatt tggcctacca aaacggttca
     2041 atctaccatg ctacgacgaa tcagcaagtg tatctgattg cattaactat gctcatgaac
     2101 ggcattttat tgcttggatt actccgacgg gaagaacatg gcatcgccaa catcggattc
     2161 gaaagttttt ttattttact tgtctatttg ggaggaatca ctattctttt ccttgcctaa
     2221 atttttcaat ctcgcttgag attgctctcg ttggaaagaa gagaagtcgg ccttggatct
     2281 ttttttactg cgttgaatgg ggagtccaac ttgaagggtc cacctttaag cgattatgaa
     2341 caaaactggc tccggccaca aaggcatgtt cctcggctag gtatcgttgt tgccaagtcg
     2401 gaacaaaacc cattagcgtc gctacgccat cttctacatg gacagagatg gagtgatcgg
     2461 atagaaatgg actccaccag agctggtctt tgatatcctg tcgaagctca ggatcggact
     2521 tatcctgcca ttcagtagca tattccagtc ggttgttgac agttacagcc cctttgacat
     2581 tggaggcaag taacccggct cgggatttct gagatcggga cttcaccgta cctttcaggg
     2641 agatggcctg attatagacc ttgacattga tgtccttatg gtcgagcaag ggatcccgga
     2701 gaaataccgc cttgacgcgt gcggcaatat cttcatcgga gtatttggga ttcatgcgta
     2761 cttttaagag gttgacgaca ttgtaaacgc cagtcgtatt ccaggtatct tcttcggcag
     2821 tcgtcttggc ttggaggtta tcgacgatgc ctgtcagagt gacgagtcct tccttcactt
     2881 caatcgtgat atccgcgttg gttattctag gatcatagga aagcgcatca tagatggcgt
     2941 cttggatggt ctcatcggat ggtatgtgaa agcctgaacg acgcatgggt tcacggtcag
     3001 accattctac tttcagttgt tcgccgttga cggatgtgat gccttgcacc caggacaacg
     3061 ataaaattct agatttctca tcaacacttc tcacggtacc gaatactgtg acctggccat
     3121 cccgaaccgc tatcctcacg gatgaaggat taatccacac atcatgattc aggcgttgca
     3181 ggatttcttg ttttatggcg ttatcagaac gattcttgac ggtcatgatc gtcaacttat
     3241 cctgaatgtc tttgattcct tccactcctt tcgccacctg taaggccaat tgcttctcct
     3301 gccatgattg aacggttccc gttaaagtga cacgcccaag cgtgacgtgt acgttcatgt
     3361 catagagctc ggtcgccggg ttttctaata atgccgattt cacctggtct tcaatttcct
     3421 gatcctcatg gcgactcgat ggattcacga gtatttgatt aataacactg cgaaccccct
     3481 tgatggttcc tgtcagtgcc gctgcccgtt ccttggccag gatgttgtcc acctcgccaa
     3541 ggagcgtgac aatacctttt tcgctgacga catcgatcag attttcctgt acaaattcgt
     3601 ctcgggccag ccggtcttcg atggccagaa tcatttcagc ctccatcatc ataggctgtc
     3661 tttgttctgc accgcttctg tcgatagacc aaaaaaggaa aatgacggtc aggaaaatga
     3721 caaccaccct cgagacttgt gtgcttgtag ggttgttgaa acggagagga cgcatagttg
     3781 gcaattccct atttagcaac actccaatat gggcaatatt gaaatgtgag ggtattcatt
     3841 gtgattgttc cctatttcgg ccaatggtta ttccgagttt tgggactttt ttaatgccga
     3901 attcaagtgt agataggctc cttcggtcaa tgttgcagcc gcggcaatgc gatcttgttt
     3961 gatatgggaa agggcatctt ctaattccga aatcccttcc ttcacaaagg cggagtcaac
     4021 cccttccttt tgtgcgagag aggcagcctt ttttgcggcc aacgcatttt tatgaaataa
     4081 atcctttcgg ccttcccccc catattccat ggcaattttt gcgtgatgaa ccgcatcttg
     4141 gaggaatcgc gtccggtcct gcaggagggg taatgtctca tcataggtga cttccaagtc
     4201 tgctaacacc gcatccattt cagtcttatt cctcaaccac ggctgaggat cgataacatc
     4261 aagatttttc gatatacgtt ctgcatgctc caattctttc gtcagtttca tgatgggggc
     4321 atacagatcc tcttgtttct tttggctgga ggcctgtgtt cgcatctttt tgagatcatt
     4381 aaggagcgca cgggatttct gtagttgttg gtggacatct ttaatgtagg gttcgggatt
     4441 ttcctgaaca tccttgacta gtttaatagg gggaatggga gtctgttcaa agctcctggt
     4501 cttcccgaat gacatatcca aaaaagagaa aatacagagg gtcatgctga tgagggtgat
     4561 gaatgatttt cgtgaaaagt tctgttgcat acttactctc ctttctcaat actctttgtg
     4621 tgggtgaaga aaaaaagtaa cccctcttag gtagaataaa aatcaggacc atactgcact
     4681 cttaaacgat tacgaacccg ttcggctccg gaatcctgcg cgacctctct ggctcgtctc
     4741 cattccacga tggaaggaac ggttcccttg agggttacgg tgccattgga cacggtaacc
     4801 gttatatcct ggtcaaaaag acccggatcc caccaaaatg cacgtctgat ctcttcccaa
     4861 atttcctcat ccaatttgcc aggcgattga atctggaggt aattcacgac atcgatgacg
     4921 ccctttactc ttgccgcggc tcgaacggtt tgggaaattt cggtaggaga attcaggtat
     4981 ccctcaagag acacttttcc actattggct gaaatcttta tttcgtagcc gtttatgagg
     5041 gggtgctgat tgaacgcttc ccggacccgt ttttccagat cgcgatctgg aaggcgcacg
     5101 ggggggcgaa ctttaatgaa actgcgtacc cgccaaatgc cttccgtatt ccgtgtcgtc
     5161 tcttcggcag cgtatttagc cgccaagtta tccacaatcc caataaggaa agccgttccc
     5221 tcgacaacac gaacgtcaat gtcagaatcc tgaaggcggg gttcatatgt gaaggctgtt
     5281 cgtatggctt ctgcggtatg ggtagttgat gtgaatgcat ctttgcgatg acgaagcatt
     5341 ttgtcccgtg cccaccactc aacgttgagt tgggccacat ttacgccttt aactccaccc
     5401 acccaggcat ctgtataggc gctattcttt tcagccaagc tccccaccgt acctgtcaac
     5461 gtcacatgtc cctggtcgac catgatgccg ataaggcttt catgcaccca tacgtcggat
     5521 tgaaggcgac ggaaaatttc cgcctcgatt tcggagtcgg gacgagaagc cttcgggtta
     5581 acggtgatcc ggcttctgag agactgaaca cctttcaccg atttgaccac ttcctcagtc
     5641 aaatgctttt cctgccaact ttctactgtg ccagtcagca tcacgcgacc ctgatcaact
     5701 tgtacggtga tttcataaga gtctgacgcc ggatctgcgg ctaatgcggt gaataccctg
     5761 aatcgtagtt ctttatcgcc aatccgcctg gtagggttca cggcaatggt attgataagt
     5821 gcctgaaccc ctcgaatgct ggctacgatt tttcctgcac gctccctggc cagcagtgta
     5881 ggaacctctc ccgacagcat gacgactcca ttatctgtgt ggacatcaat tccatttgag
     5941 gggaccatct gatcgaccag gaaacggctt tcaattgcga cggtaatatc tttgtccgta
     6001 atgacatttt gatccacttc ggccaaacca atggatggca aggtgaccag ggatatcatc
     6061 acccataaaa aaagcgatat gagggattgt cgaaaaatgt ttctcatctg cccatctgat
     6121 ggttcttgag tggtccctga attgtgatat ctctttttaa gcaagaaatg accttctttt
     6181 tttttacaag aaatggcctt ctttgttgtt aaaatccgac gttacggtaa attgggttga
     6241 cttgaattaa gctgttggtc tgacaaaaaa ggcgtagcct ctttgtctta tataaacgaa
     6301 aacggaaaaa atgggtccta tgcaaaaccc tggatgtctg ggatgcgaag gtattttaga
     6361 ctcggtgcgt catgctggag agattgtcgg aaagtcattt ttgggagtag acatcaattc
     6421 aacgggacgt ccatcaaaag cgtttgctga gagagcattg acaaaactac catacattaa
     6481 aatgcatgac cattggcatg tattctttta tcgttgttgt ggattggacc acgatctgat
     6541 cggcaccgtc gagatcgaat ttttgggtga gccttcaatc actttatcgc tgtagacgag
     6601 atatatgaat gtctgacgct tcttgtcaaa gaaacggacc acctgcagtt ttttaaatat
     6661 cagtgatcga ctctcgtgaa atactttctc tccttcttca agctcatcaa ttatgcgaat
     6721 gggaccgacc tgtcggcagg caattgaggc atctgaagtg tcctcagcga caccaaccat
     6781 gcctttcaag ccccctgtct tcgctcgact cagatggcag gtgattcctt caatcttggg
     6841 atcatcaaac gcttcaatca caattttgtg atcaggaccc aaaaaattga attttgtatc
     6901 cacacttccg atttcttccg catgactcaa agagaggatc agagccgaaa agaatagaat
     6961 gtttaagaaa attgtcagca tgtaggcatt ctcccttacc cgatttttac catgtccaat
     7021 atgaataatt tgatgggctt tactttaccc tatagtcctt aggactcaaa accggataaa
     7081 tgttgaaacc ctgcatcaag aacgtccagg gctagacgtt caagagccac aaggggttct
     7141 tgtttttgct ccgcggcgga gcgcccatta taggccccct ccttgaagac tatcgatcac
     7201 cagccggaca ataaaaaaat acaggataag gctcgtcaca tccgtcgtag atgtgaccat
     7261 ggggctactg gcgacagcag ggtttatacc tactttggtc aagaggacag gaaataatac
     7321 accgagaaga ttggagacca caacgactgc aatcattgct aatcccacac ctactcccac
     7381 aactatccct ccccatgtgc aatcaactct tttaccatag atatctccat gtacgactcc
     7441 ttccagccgg tctagccaat gactgacgag tttaacgtat tcgacatttt ttaaaataaa
     7501 gggaaaacat catttttcca gaaccctcca cgggcagtgc acagagatac gaaaaggaat
     7561 ttttcagttt ggtgaatata gacaacaaag gaaacaccag ggttcgggtc gtcctgtcac
     7621 ctattcgtgg taaggtccat cggtgacagt tgatggaaag aaatcctaag taagatttga
     7681 tcaggccgtc ctgtctatga aacgacaagg cttaagccgt ctgtctatag tcgatcacaa
     7741 ccgaatcgtt ggaattctcg ctaaccagga cattgatatt gataatactc tttggggttg
     7801 ggacctcacg aagacttgca aattcatatt ggtagtcttt gaagtctcct gacacgccaa
     7861 tgtcagactt gcaagcggcg aaaactctgc ccttatcgta ggaaatggag gatgtggatc
     7921 atcgctcaga ggagtcgtaa ggaatctctt gggagaaaat acccttttcc gaaggagagg
     7981 cttgataggt ggattcttcg tcctccaacc gcatctcatc ataaatgagg tacccgttga
     8041 ctgctcgagc ggattcgcga acccatccca tggctaagag aatgatcacc gtcaaccctg
     8101 ccaacgttat caacaaaccc gccatccttc tccgggctcc ggacacggct ctcgatacgg
     8161 tggcttgtgg cattcgccca aacacccgaa aggagtacag ccaatgtgca aatccaaaga
     8221 tcacaaggct tgaaagcgca aaatatttat acggttgcat cttcccaaag gaattaattt
     8281 cctgatcggt caaccgattg agaagaggga tatgctgcaa ttgatagggc atcgccaaaa
     8341 tcatagcggc gatcacgatg acagtgagtg acaagggaat gaatccgatg aaagatggag
     8401 gtggttttga ctgcgaccga accatctgca tcacatacag aaaaccgacc agtattaata
     8461 atccatacaa caacgccacg acatcaaaaa gccacgaacg ttctcccagc atcacggtat
     8521 aaaaggcctg gggttgtcca tatcgcaaat gaagcaaata ttgatatccc acgaccggca
     8581 tgaccagcag agctccatag ccgattaaaa aacagtaact tcctccttta tggaaaaagt
     8641 ttcgatctgt atcaattgta gatttcagat aacggacggc acagatccca cccagggcaa
     8701 aacccgacca agtcagattg ccaaaccacc ggtgaagatc caaatgaatc atggtgggat
     8761 tgaacacatt ctcccatgcc gaatgaatgg gatccggggt cagcatatag gaagcggtga
     8821 tatcgatcat aaacatggca cttaaggcac agcctgcggc aagccatata aaggccaagt
     8881 gcaccttttg tcttaaccca tgccgtcccc attcccagag gtaataccag gcataggcaa
     8941 aaatcgcttc tcctaaaaac aatgccgcct ctgccaaaaa cggccaaaac atcacgtgaa
     9001 acaggcggtg ccagaattga ggccaataga tgttcagagc aaacaccaac acgacgccaa
     9061 aaaatgaaat ggtcgcagtg gaaagcacca atgcaaaggc aatcaacttg gccaaccggt
     9121 ggaaccattc ttcttccgtt acatagctaa atgttgctgt cacggctccg atcaaggatg
     9181 aacctacaat gaaggtggca aataaagtat gagtcagcgc agatgcggca atcgccacac
     9241 gcccgacggt catagaagtt tcaagtggct caagggtcat gggaatgtct cgcctctaaa
     9301 tgtcgattcc gtctgacagt tgataattcc atgaatggta tccggccctc tcgccacttc
     9361 ccggatggtt cccattgtca aatagaggac gagcgcggct agtccaaggg ccatggcgat
     9421 gaagtgaatg aacggcttgt tgagtgaggg cgttgagcca cgcatccaaa caggaaacac
     9481 atacagaaag taccaacccg tttgaagaag cacgataagg ttcacgatac gccgatacca
     9541 aggatacggc gccagaacga ccattaacac gaccgtcagc acataaaata cctccccccg
     9601 gaaggaatgc cctcgctcca gatgcaccga gcgaaaaatg agatgactgc ctaataggag
     9661 tagagccagg agggtaaatt gccaaatcac caacaggact gtttggcctt catagaggtc
     9721 ctgcagaatt tttggctgtg cattgtgtat gtgttgcgaa aatacgaaac cggagaccgg
     9781 ctgaatcatc aggcttacaa tgccgatggc catgcctgtt tttatgagcg tgaggtaata
     9841 ggcatcatcc ttcttttgac ccgattgttt ccttagacgc catccggcat acgccgccat
     9901 gacgtatccg gcgatgacca gatttccaaa gaatcgatgg accatcaagg gtacccatgt
     9961 cgggttccag agccttgccc atccgctatc gccttcaaca ggagtcatca tataagaacc
    10021 gatcccgtca agaattcctc cccatatcag aattaatacg gcggctaccg tacccaacgt
    10081 gatatgccag gtgatgcttt tcgaacgaag ccgatcccaa aagtgataat agggatagag
    10141 gcacagcact tgaatgaaaa acacgatcaa agccagatgc aagggatagc ggaagtgatt
    10201 aaacaggtaa ctattcgtca gagggaaaag tccgatgaat aattccagca tgaagacagc
    10261 caacacgatg ctggtggtat acgtcaccag cgtaaatcgt gtggccagat acgcaaaatc
    10321 cgtaaaatag ggacgactgc gtcccaatcc ttccgcgatg ggagccagaa ccatgaatcc
    10381 caccccaaga ctcgcgaggg taatatgggc caaactgaaa aagccgatcc aaaagcttga
    10441 actcgtcatc atgtttacca catgtccaaa gacgcgttaa ttaacccggt gtattcggtt
    10501 tctgcatcaa gcgactggtg gatggcgcat gttccgaaaa agtccgaagg aatgccacca
    10561 aggcccaact ttcttcctga gaaagaaagg gttcaaacgc gggcatcaca ttttctccat
    10621 gagtgaca
//
LOCUS       DATIAL010000067         6823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_227401, whole genome shotgun sequence.
ACCESSION   DATIAL010000067 DATIAL010000000
VERSION     DATIAL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227401"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..2088)
                     /locus_tag="VLA60_02210"
     CDS             complement(10..2088)
                     /locus_tag="VLA60_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSF08195.1"
                     /translation="MSTKICALMFEQSGSVWIFSALVLISFFPSVTTAADSLAEESDF
                     SQATAQAAHEATAPVYQLDPVVVTATKTPVPLTQTTSAVEVITEENMQRRHSRTLVDA
                     LNLSQGLATFSSGGPGTNNTLRLRGGSSAQTLVLIDGAIVNSATLGEFNFGTVTTDNI
                     ESVTVLRGAQSMLWGSDAVGGVVDIRTKRGTGDPVARIFSEYGSFNSIREGGSFAGQM
                     GPVDFSVTLTRWDMTKFSSVNYRRGATERDAFRNWQASSLLGVKGPWDGRLELAFRWI
                     NNDIDLDNPSTFGGPYDVFKAKSTGEQFIYSGSYHQPITDWWDQKITLSHAQETLITQ
                     AGDNQRSITTGLESTPGAFNNSDIRTKSNRIEWQSNFQIGKPLLLTVGYQFREQRGEN
                     KGTFSEKTLSSNAGFAQLQLNLFDRFFATGGFRQDSYNSFGDATTYRVTGGYLIKETG
                     TKIRTSYATGFRAPSINELFFPNFGNPNLKPEKSQSFDVGVDQVFWDKRVTLSAGYFW
                     NRYRQLIVTAFDPAGCAPFSPFGFCAQNLGSAKSQGWEGSAKLVLAEGLPYLKLLDLQ
                     GQYTYTITRDLETGARLPRWPVHQGSVVLTYQPIAPLSMTTTFRYVGSRFNTTGDQQP
                     LPDFYLINFSASYDITPTLQGYVRVDNILNRHYEEVLYFGTPVRSVFGGVRVTFDLPV
                     SKTSSASGNS"
     regulatory      complement(2117..2310)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2361..3191)
                     /locus_tag="VLA60_02215"
     CDS             complement(2361..3191)
                     /locus_tag="VLA60_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF08196.1"
                     /translation="MMDSGNLLTGPAYALRDVTFGYDTRFRDHSESVLKSVTCSIDSG
                     KILGILGPNGSGKSTLLKLLARVLCPRSGTVELFGDPLSRLSQVEVARQVALVPQETL
                     QIFPFTLAEMVLMGRFPHHQGYGGWHWEDADDWRIAYQAMQDLDVVHLGSRLVSDVSG
                     GERQRAVIARALTQVPQVLLLDEPTAFLDLHHQLDIARILRRLNRERGLTVILVSHDL
                     NLASQYCDQLMMLHHGKIMEVGSPRDVLRPDLLESVYGCQVLVDRHPQSGLPRVSLPV
                     "
     gene            complement(3191..4333)
                     /locus_tag="VLA60_02220"
     CDS             complement(3191..4333)
                     /locus_tag="VLA60_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HSF08197.1"
                     /translation="MVKVPPFTLSPSSKVSSEWDQASPPLPFAISSLPALTIRGWLLS
                     VGVSCVLAIVTLLVCLGFGTEPIPFPRIWSILVGALPGTESIRVGTDPTTVILLQVRL
                     PRLLLAFFVGGSLAMVGVALQALLRNPLADPFIIGISSGAALGAAIALLFGVGVSVLS
                     VSALPVCAFAGALLSLVIMYRISSVGFGFSIYTLLLAGVVLNAIFSAFIMFLTSVADP
                     NRAFGMYAWLMGSLTGPDFPTLGVLALYLGMGLVILATQAQSLNLLTLGEETARSLGV
                     EVERVKKLVFVAAALLTGAVVSFSGIIGFVGLIVPHAVRLVLSADHRLLLPAAGFVGG
                     MFLMLADTVARTALSPSEFPVGVVTALVGGPVFLYLLTTRNLRVGM"
     gene            complement(4453..5502)
                     /locus_tag="VLA60_02225"
     CDS             complement(4453..5502)
                     /locus_tag="VLA60_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSF08198.1"
                     /translation="MKNRVQSSRLCKPWGFFILSQCLRHLVFGIVAGWAIFHCAFSGD
                     VVLAFEGEMMKRRQQGILTGMPFMANVAPRTFVDDLGRKLYLAKAPQRIVSLAPSVTE
                     ILFAIGLNEEIVGVTEFCDFPPEALTKSKVGYATPNLESIVGLQPQLVLAPRSFLRVD
                     LLNKLEQLKIPTFIFDPHTVEDIFAHIQLLGRMVGHSEEANAQAALMRKQLSNLSSRL
                     ADLPRPSLLYVLNSEPLITVGPGSFIHRLIELAGGRNAADQTSAPYPRLTMEEVLRQN
                     PDILLFPTGRHEGIPQVEQDAWRRWTTLSAVQHGRLFQVDSDLLNRPGPRIIEGLKQL
                     AKILHPEVNQDDMSN"
     regulatory      complement(5552..5692)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6214..>6823
                     /locus_tag="VLA60_02230"
     CDS             6214..>6823
                     /locus_tag="VLA60_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSF08199.1"
                     /translation="MLNTVRIALLHLAPVPGDLIENRRLIERGIAVAARAGAAWIITP
                     ELCVCGYTFTDTIGTDWIMPQPDPWVSRICQMAARLRVTVFLSHPERDSQSNALYNTV
                     FVIASDGAIAGSHRKINSLKTGSESWATPGSIVAPVPVQSFKHAGILICADAFSPGIA
                     GSLAEQGARVLVSSAAWAPGFHGPNGEWERCTLDTGLLLLVCN"
BASE COUNT         1756 a         1938 c         1634 g         1495 t
ORIGIN      
        1 tcggaaaggt catgagtttc cacttgcgct ggaagtcttt gataccggaa gatcaaacgt
       61 cactcgaacg ccaccaaaca ccgaccgcac cggcgttcca aagtacagca cttcttcata
      121 atgccggttc aggatattgt ccacgcgcac atacccttgc agggttggcg taatatcata
      181 cgaagccgag aagttgatga gataaaaatc cgggagtggc tgttgatcgc cagtcgtgtt
      241 aaatcgggaa cccacatacc ggaaggtcgt ggtcatactc aaaggcgcga tgggttgata
      301 tgtcaacacc acgctgcctt gatgaaccgg ccagcgaggc agcctggccc ccgtctccag
      361 gtctctcgta atcgtatagg tatactgtcc ctgaagatcc aatagcttca aataggggag
      421 cccttcagcc aggaccagct tggcgcttcc ctcccatccc tgacttttcg cagatccgag
      481 attctgcgcg caaaatccga aaggggagaa tggagcgcag cctgcagggt caaacgccgt
      541 gacaataagt tgtcgatagc ggttccagaa atatcctgca ctcaaggtga cgcgcttgtc
      601 ccaaaatact tgatcgaccc caacatcgaa gctctgactt ttttccggtt tgagattcgg
      661 gttcccgaaa ttcggaaaaa atagttcatt tatcgatgga gctcgaaatc cggtggcata
      721 actggtccgg attttcgtac cggtttcttt aataagataa cccccggtca cacgataggt
      781 cgtcgcatcg ccgaacgaat tataggaatc ctgtcggaat cccccggtgg caaaaaaccg
      841 gtcaaacaag ttcaattgga gctgtgcaaa accggcatta cttgagagag ttttttcaga
      901 aaacgtacct ttattttctc cgcgttgttc tctgaattga tagccaacgg tcagaagaag
      961 aggcttcccg atttgaaaat tactctgcca ttcaatccga ttgcttttgg ttcgaatatc
     1021 ggaattatta aaggcaccag gcgtactctc caaccctgtg gtaatgctac gttggttatc
     1081 ccccgcctga gtgattaacg tttcctgggc atgcgagagg gtaatcttct ggtcccacca
     1141 gtcagtgatg ggttgatggt aactcccgct atagataaat tgttccccgg tggacttcgc
     1201 tttgaacaca tcgtagggac cgccaaaggt gctgggatta tccaagtcaa tatcgttatt
     1261 gatccagcgg aatgccaatt ccaaccgccc gtcccatggc cccttcaccc ctaacaacga
     1321 agacgcctgc caattgcgaa acgcatcccg ttcagttgcc ccgcgccggt aattcactga
     1381 agaaaatttc gtcatatccc aacgcgtcaa tgtgacagaa aaatctaccg gtcccatttg
     1441 tccggcaaaa ctccctcctt ctcgaatgga attaaacgaa ccatattcag agaagattct
     1501 ggcaaccgga tcacctgtcc cgcgcttggt tcggatgtcg actaccccac cgaccgcatc
     1561 cgaaccccac agcatgctct gcgccccccg caggaccgtc acggattcga tattgtccgt
     1621 ggtcacggta ccgaaattaa attccccgag tgtcgcactg ttcacaatgg caccatcaat
     1681 gagcaccaga gtttgagccg aacttcctcc acgaagcctg agcgtgttat tcgtgcctgg
     1741 cccaccagag gaaaacgtcg ccagaccttg gctcaaattg agcgcatcca caagcgttcg
     1801 actgtgtcgg cgctgcatgt tttcttctgt aatgacttca accgcactcg tggtttgggt
     1861 aagaggcacg ggtgtttttg ttgccgtcac caccaccgga tctaactggt aaacaggcgc
     1921 ggtagcctca tgggcggcct gcgctgtagc ctgcgagaaa tccgactctt cagccagact
     1981 atcggcagcc gtggtgacgg aaggaaagaa agagatcagc acaagagcag agaaaatcca
     2041 aacactacca gactgttcaa acatcaacgc acaaatcttc gtggacatga tccacctccc
     2101 tttcgctcga aggggtttta tggaagaaac agctgttggg tctcctgact tgcggatcct
     2161 cgtgtttctg cgccttctca tggacatctt gtccacaatg gactcttgca gaattactcc
     2221 ccgcttacag tggcggcacc gtgctggatt ttcaccagct tccccgtcac aaccgtcgtt
     2281 tttcctctaa ataaccctac tcctttatta aatggttaat accgataccc gtacacaaca
     2341 cacggctttg gtccactcgc tcacaccggc agcgacaccc ggggtaatcc cgattgtgga
     2401 tgacgatcga ccagaacctg gcagccataa accgattcca acaaatcagg acgcaagacg
     2461 tcgcgaggag atcccacttc catgattttc ccatggtgta acatcatgag ctggtcacaa
     2521 tattgactgg ccaggtttaa atcatgagac accagaatga ccgtgagacc acgctcccgg
     2581 ttcaacctcc gaagaattct ggcaatatcc aactggtggt gcagatccaa aaaggcggtc
     2641 ggttcgtcca atagcaatac ctgaggcacc tgcgtcaggg cacgagcgat caccgcccgc
     2701 tgacgttccc ctccggacac atctgagaca agcctggaac ccagatggac gacatccagg
     2761 tcctgcatgg cctggtaagc gattcgccaa tcgtccgcat cctcccaatg ccatcctcca
     2821 taaccctgat gatgcggaaa tcgtcccatc aggaccattt cggcaagggt aaatggaaaa
     2881 atctgcaggg tctcttgcgg aaccaaggcg acctgtctgg ccacctcaac ttgggacaga
     2941 cgggagaggg gatccccgaa caactccacg gtaccggacc ggggacataa aacccgggca
     3001 aggagcttaa gtaaggtact ttttccggaa ccattagggc ccagaattcc aaggattttt
     3061 ccggaatcaa tcgagcatgt gactgatttc agaaccgact ccgagtgatc gcggaaacgg
     3121 gtgtcatagc caaaggttac gtctcgaagg gcatacgccg gtccagtaag taaatttccg
     3181 gagtccatca tcacatcccc acccgcaggt tacgagtggt gagtaggtat aaaaacaccg
     3241 gtccccccac cagtgccgtc accacaccga caggaaattc actgggactc aaggctgtcc
     3301 gcgccaccgt atcagccaac atcaaaaaca tgcctcccac aaatcccgca gccgggagca
     3361 aaagacgatg atcggcactc aagaccaggc gaacggcatg cggcacgatc agccccacaa
     3421 acccgataat cccgctaaaa ctcacaaccg ccccggttag aagcgccgcg gcgacaaaca
     3481 ccagcttttt gactcgctca acttccaccc ctaacgatcg ggccgtttct tcaccaaggg
     3541 ttaggagatt taacgattgc gcttgtgtcg ccaaaatgac caaacccatt ccaagataca
     3601 aagccaggac acccagtgtc ggaaaatcag gcccggttag cgatcccatc aaccaggcat
     3661 acatgccgaa cgcccgattg ggatcagcca cactggtcag aaacatgatg aacgcggaaa
     3721 aaatcgcatt cagcacaacc cccgccaaca ataacgtata gatcgaaaaa ccaaatccca
     3781 cggatgaaat ccgatacatg atcaccaaag acagtaacgc accggcaaag gcacataccg
     3841 gcaaagccga cacactcaaa acagaaaccc ctacgccaaa caacagcgca atcgcggccc
     3901 ccaatgccgc cccgctcgag atcccgatga taaacggatc ggccagggga tttcgcaaca
     3961 aggcctggag tgcgacgcca accatcgcca gactgccccc aacgaagaat gccaaaagca
     4021 gccgtggcaa gcgaacctga agcagaatga ccgttgtcgg atctgtcccg acacgaatgg
     4081 actcggttcc cggcagtgct ccaaccagaa tggaccaaat ccgtgggaaa ggaatcggct
     4141 cggtcccaaa tcccagacag acgagaagtg tcacgatagc caacacacac gaaacaccca
     4201 cagacaagag ccacccacgg atcgtcagtg cgggcaatga tgatatcgcg aatggcaagg
     4261 ggggagatgc ctgatcccac tcactcgaca cctttgaaga tggggaaagg gtaaatggcg
     4321 gaacttttac catgggcttc accaacaggg ctttcttctt ggaatcgcta aacacgactg
     4381 cgtaatcgat tcaggagcga aaagaagatg atgggctccg tggcccgatc gtgatgtttc
     4441 aattgggagg tttcaattcg acatgtcatc ctgattgacc tccgggtgca gaatcttcgc
     4501 taattgcttc agcccttcaa taattcgtgg gccgggacga ttcagaagat cgctgtccac
     4561 ttgaaacaac ctgccgtgtt ggacggcgga gagcgtcgtc caccgtcgcc aggcatcctg
     4621 ttccacctga ggaatccctt catgccgacc ggtcggaaag aggaggatat ccggattttg
     4681 tcgcaagact tcctccattg tcagacgcgg atagggcgca ctcgtctgat ctgccgcatt
     4741 tctaccacct gccagttcaa tgaggcggtg aataaaacta ccggggccta ctgtgattaa
     4801 tggttcggaa ttcaagacat acagtaatga gggacgaggc aggtccgcca atcgacttga
     4861 taaattgctt aattgcttcc gcatgagggc tgcctgggca ttcgcttctt cagaatggcc
     4921 taccatgcgt cccaacaact gaatgtgggc aaaaatgtct tcaaccgtat gcgggtcaaa
     4981 aataaaggtg ggaatcttca attgttccaa tttattgaga aggtccacac gcaggaacga
     5041 tcgaggcgcc aagaccagtt gtggttgcag acccacaata ctctcaagat tgggagtggc
     5101 ataacccacc ttcgacttgg tcaatgcctc gggagggaaa tcgcagaatt cggtcacccc
     5161 cacaatttcc tcattcaacc cgatggcaaa cagaatttcc gtgacacttg gggcaaggga
     5221 gacaatacgc tgaggggctt tggccagata gagctttcgg ccaagatcat cgacaaacgt
     5281 gcgtggagcc acattggcca tgaagggcat acccgttaaa ataccctgtt gacgccgttt
     5341 catcatctct ccctcaaagg ccaacaccac atcaccggaa aacgcgcagt gaaagatcgc
     5401 ccacccggcc acgattccga agaccaaatg gcgcaaacat tggctcaaaa taaaaaatcc
     5461 ccaaggctta cacaaccttg aggattgcac ccgattcttc atcctcacca cttccccagc
     5521 ccacgaggga acattggtct tggaacgaat acggtattct gacttctgag tgattaccta
     5581 ctatccgcgc cttcccatcc gaaacacacg cacagatcgg acagtggctc tcgcggggtt
     5641 cgtcctcagt tacagcggcg ggaccgcgcg ggaatttcac ccgcttccca ttattcgttc
     5701 cgcatagttt tgggagaatg taggagggac gaagccgctt tgtcaagaat acacagttaa
     5761 tgaataattt gcccggcggt atttttcaaa cgccacctgg ggcgttggta ggaggacaat
     5821 gcctcaagat actctttccc tgctctcatg cgccgcccgt gacacatcca tacgctctaa
     5881 aattcccggt ggtacttatg ctctttcttt ttgcgccatg tcaatccttt cattgcccga
     5941 tccggaattt cactctgata gacgtcaagc aagatgggat tttcaagaac ccgtcatagg
     6001 ccaggtcgga tgcctatagg gctgttgtgt cgtcttgctg tcactatcat ttttcaattt
     6061 ttacttcgga catccagaga aaacagactt atattaaaca ccttcttatt acgtacctgc
     6121 ctacaaattc tttgatggtg cagcctttgt gggcgtatgt tacgttggcc ggagcagcca
     6181 ttcctcacgt caatcttcag gtcaggagtc agagtgctaa ataccgtgcg tatcgcattg
     6241 ctccatctcg caccagtccc gggcgacctc atagagaacc ggcggctcat tgaaaggggg
     6301 atcgcggtcg ctgcacgcgc aggagctgcc tggattatta cccctgaact ttgtgtctgt
     6361 ggatacacct ttaccgatac aatcggcacc gactggatca tgccgcaacc cgatccctgg
     6421 gtaagccgaa tctgtcagat ggcagcccgt ttgcgtgtca ccgtatttct gtcacatccg
     6481 gaacgggatt ctcagtccaa tgccctgtat aacactgtct ttgtgattgc gtcagatggc
     6541 gccatcgcag gctcccatcg caaaatcaac tccctcaaaa caggttctga atcgtgggcg
     6601 actcccggaa gcatagtggc tccggttccc gttcagtcct ttaaacatgc tggcattctc
     6661 atctgtgccg acgccttctc gccgggaata gctggaagcc tggcagaaca gggagcccgg
     6721 gtactggtct cctccgccgc atgggcaccc ggttttcatg gtcccaacgg cgaatgggag
     6781 cgatgcactc tcgataccgg actccttcta ctggtgtgta atc
//
LOCUS       DATIAL010000068         2737 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1087416, whole genome shotgun sequence.
ACCESSION   DATIAL010000068 DATIAL010000000
VERSION     DATIAL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087416"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VLA60_02235"
     CDS             <1..1145
                     /locus_tag="VLA60_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF08200.1"
                     /translation="GFTSKHVYYYCGDNVSAEPEYVDEGLARFRYTFPDGEAFHLNMF
                     PLRKEYTRRLMNEVGFQQVKTYADFQETHKVEDPDFYVHVAEKLYKNEERQISILANL
                     TNHGYSPTVDTAREYYNSSDADRFYATIWGGEDIHIGLYNSENDSIFQASRRTVEKMA
                     SLVKGLGSTTRVLDIGSGYGGSARYLVKHYGCQVGCLNLSDIQNKRNRELNRKEGISL
                     AINVIDGSFENIPMPDGSVDLVWSQDAILHSGDRKKVFDEVYRVLAKGGQFLFTDPMQ
                     SAKCPPDVLKPILDRIHLDSLGSIEYYQQLAIDRGFKEIRTVDLSPNLPAHYTRVLQE
                     LIAQHHALIRVCTEEYLSSMKVGLQHWVDAGQAGHLTWGILHFQRD"
     gene            complement(1205..2593)
                     /locus_tag="VLA60_02240"
     CDS             complement(1205..2593)
                     /locus_tag="VLA60_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08201.1"
                     /translation="MSNSVQDWLDALEDVDDATREEAAKALADKGDPKTLEPLLTALE
                     DDFWSVRSHIGWALAKIGGEKAIEGLITLFNDNMMEVQAEAVQAMASMGKEVIPHLLT
                     GLKDKRWRVREQAAKTLGELRDPQAVQGLILVCRDRDGAVKSAAAEALGKIGDARAVP
                     TLIKLFKDTSKIVRETAGTALMYVGEKSVEALLESLKDPHFVVRCHAVRALGGMTTDY
                     QMGRVWVREPRVVDALIEMLKDPDRAVREDATIALGNIGDPRAVDALMEAMKDGAVKR
                     HAIASLGMIGDPRAYPPVLDALKGKGIHQAGKPTPGCIISEDLLIKEAAATALGHFRH
                     PKVIPDLVLLLKDLVLRDYAANSLVLIGDAAIEPLVWFLHDPELSKVEKESERVLAFA
                     TTRMTASGALKKTILDTLDKLGWHPTEGTSKETREIEYTDTSNVLGEGGEGRFGKSGD
                     FAMPAKPPKIER"
BASE COUNT          652 a          672 c          689 g          724 t
ORIGIN      
        1 acggtttcac ttcgaaacat gtgtattact attgcggtga caatgtcagc gcggaaccgg
       61 aatatgtgga tgaggggctc gcccggttta gatatacgtt tcccgatggc gaagcctttc
      121 atctcaatat gtttccgcta cgaaaagaat atacgcgtcg gctcatgaat gaagttggtt
      181 ttcagcaagt gaaaacctat gccgattttc aggaaaccca taaggtggag gatcctgatt
      241 tttatgtgca tgtggccgag aaactctata aaaatgagga acgtcaaatt tccattctcg
      301 ccaatttaac aaatcatgga tattcaccca cggtggatac ggctcgtgaa tattacaaca
      361 gttccgatgc tgaccggttt tatgccacga tttggggtgg cgaagacatt catattggac
      421 tctacaattc cgaaaacgat tcaattttcc aggccagccg acgaaccgtg gaaaaaatgg
      481 cctctttggt aaagggtctt ggctctacaa cacgggtgtt ggatatcggg tcgggatatg
      541 gagggtcggc gcggtatctc gtgaaacact atggatgtca ggtgggatgc ctgaatttga
      601 gtgacattca aaataaacgg aaccgagaac tcaatcggaa agagggtatc agtttagcga
      661 ttaatgttat tgacgggagc tttgagaata ttcctatgcc ggatggaagt gtcgatctgg
      721 tgtggtccca ggatgccatt cttcatagcg gagaccgcaa gaaagtcttt gacgaagtgt
      781 accgggtatt ggccaagggt ggacagttcc ttttcacgga tcccatgcaa agtgccaagt
      841 gtccgccgga tgtgttgaaa cccattttgg accgaatcca tttggattcc ttgggatcta
      901 ttgaatacta tcagcagttg gccatagatc gtggattcaa agaaatacgg actgtggatt
      961 tatctcccaa tttaccagcg cactataccc gggtacttca agaactgatt gcgcaacatc
     1021 acgcgctcat tcgtgtgtgt accgaagaat atctttccag tatgaaagtg ggattgcagc
     1081 actgggtcga cgcgggtcag gcagggcatc tcacgtgggg aattttacac tttcagcggg
     1141 actaataggc gggagaaact catcgacggg tgttcgcctt cctccgaaga gaggaaggcg
     1201 gatgttatcg ttcaattttc ggtggtttgg caggcatggc gaagtcaccg ctcttgccaa
     1261 aacggccttc ccctccctca cctaatacat ttgaagtatc cgtatattca atctccctgg
     1321 tctctttgct ggtgccctct gtgggatgcc atccgagttt atccagggta tctaagatgg
     1381 tttttttgag ggcacccgac gcggtcattc gtgtcgtggc aaacgccaga acccgttcgc
     1441 tttctttttc gactttcgac aattccgggt catggaggaa ccataccaat ggttcaatgg
     1501 ctgcatctcc gatcaatacc aaggaattgg ctgcgtaatc tcgaaggacc aggtctttga
     1561 gcaacaaaac caaatcggga atgactttgg gatgacggaa atgaccgagg gccgttgcag
     1621 ctgcttcttt gattaaaaga tcctcactga taatgcagcc gggtgtcggt tttcctgctt
     1681 gatgaatgcc ctttcccttc aaggcgtcca gaacgggagg ataagcccgg ggatcaccaa
     1741 tcatgcctaa agaagcgata gcatggcgtt tgactgcgcc gtctttcatg gcttccatca
     1801 gagcatcgac cgctcgggga tctccaatat tccccaaggc gatggtggca tcttcgcgta
     1861 ccgctcgatc tggatccttt aacatttcga ttaaggcatc gacgacgcga ggttcgcgga
     1921 cccacacacg gcccatttga taatctgtgg tcatcccccc taatgcccga accgcatgac
     1981 agcgtaccac gaagtgggga tccttgagag actccagaag tgcctctact gatttttccc
     2041 ccacatacat caaggcggtt ccggctgttt ccctcacaat ttttgaggtg tctttgaata
     2101 atttgatcaa cgttggaact gcccgcgcat ctccaatttt gcccaaagct tctgctgcgg
     2161 cactcttgac cgcaccatca cggtcgcgac acaccaggat caatccttgg acggcctgtg
     2221 gatcacgtaa ttctcctaac gttttggctg cctgttcgcg cacccgccat cgcttgtctt
     2281 tcaagccggt cagtaagtgg ggaatgactt ctttccccat ggaagccatg gcctgaaccg
     2341 cttcagcttg cacttccatc atattatcat tgaagagtgt aatgaggcct tcaatggctt
     2401 tctcgcctcc aattttggcc agtgcccatc cgatgtgaga acggaccgac caaaagtcat
     2461 cttcgagggc tgttaataaa ggctctaatg tcttcgggtc acccttgtcg gcaagagcct
     2521 tcgcggcttc ctcgcgagtg gcatcatcga catcctcaag agcatcaagc cagtcttgca
     2581 ccgaattgga cataatcatc cttcttatcc catcggatta tttggggtaa tccggttcct
     2641 ggggataggg ttgggtatgg ctacaatgaa tcgtcaggac ttgcgaccct cttccatcca
     2701 cgcattgatc gagtgttggg gagtattcca gggtccc
//
LOCUS       DATIAL010000069        10188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_442318, whole genome shotgun sequence.
ACCESSION   DATIAL010000069 DATIAL010000000
VERSION     DATIAL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442318"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VLA60_02245"
     CDS             <1..195
                     /locus_tag="VLA60_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08202.1"
                     /translation="IGRLLGGPRDGRFLYCGPCLSAASWPAPHLASVLSNVAGLGVIG
                     FGYFCRTKSSSAAGPKPGTF"
     gene            complement(317..1189)
                     /locus_tag="VLA60_02250"
     CDS             complement(317..1189)
                     /locus_tag="VLA60_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HSF08203.1"
                     /translation="MHSPSKTKIQALLRLLSDPNEQVAKTIQHEFIRIGPSALPFLEK
                     AGADDTALESRVAFVKDEILFEKLKEEFSTFVTHCSRQMDWEQGAFLIAKIAYPDVDI
                     PHYQQCLDQLTEEFRKKWHSEGSAPGKVARLLSAFLFKDKGFSGNRIHYHDPDNSYLH
                     RVIESRQGIPISLSALYVFVGNRLNLPLSGVGMPGHFLVKIEGEPIPQFVDCFNGGAF
                     LREQDCEQFITASGLDYSPEFLEKSPTRLILARMLRNLLSIYEREQQNPMTKRVETLV
                     QILQEASEDEDPSL"
     gene            complement(1277..2056)
                     /locus_tag="VLA60_02255"
     CDS             complement(1277..2056)
                     /locus_tag="VLA60_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF08204.1"
                     /translation="MNEQIQEVLALTNRWVKRLSREKFSMLFTLIQPMLFWLIFFGNL
                     FQRAADIQVVQAPNYMSFLAAGVVVMTVLNNGLAGGVDLLFDKENGFLERLMATPIRR
                     SSVILSRFLFVMAITAMQILVILGVAFLFGVSAETGILGIALILVIGMLFGVGLTAIS
                     MAMAFSVKSHGDFFSVLGFLSLPMIFLSSALVPLAAMPGWMKSLAFFNPMTWVIDAIR
                     PLILTGWSEALPQVAIALGVLALFDALCLYGGARAFRRSVG"
     gene            complement(2100..3074)
                     /locus_tag="VLA60_02260"
     CDS             complement(2100..3074)
                     /locus_tag="VLA60_02260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF08205.1"
                     /translation="MSDAVAIEVKGLGKTYDTVTAVKNLSLSVMKGEIFGLLGPNGAG
                     KSTTLRILITTLSPTSGTATIFGHDVAKEADTIRRMIGYVPQERAIDRFLTGREHLLL
                     LADLYHLPKNEALTRIASLLKLVDLEEHADRVAKGYSGGMKRKLDIACGLLPNPKVLF
                     LDEPTLGLDVQSRLNIWQYLRQLREQGMTIVMTTNYLDEADQLCDRIAIIDRGEIRAI
                     GSPKDLKASLGGDGVWLTLGNPQPQVIETLSTALKALPFVRAIRPTPQGLDIRVDAPE
                     KALPAILEATNRVGCLLDGIEYHRPRLDDVFVAHTGRSLKDQPSEPTT"
     gene            complement(3080..3739)
                     /locus_tag="VLA60_02265"
     CDS             complement(3080..3739)
                     /locus_tag="VLA60_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HSF08206.1"
                     /translation="MPSDFPSAEFGKFPIPDSVPIFPLPNVVFFPETYLPLHIFEPRY
                     REMVHNASGQGDCIGMVLLKDGWEDEYEADPPIYPIGCVGRIISSHKLADGRFNIVLQ
                     GLQRCTFDEQEVSTPYRQARIIPNAQKGAPSLDIETRINLEQITQEYLTWKKAHELCQ
                     VIASGKLTDSILVHNLSAGLDLSPVEKQFLLESGHLIQQARRLIDLIRFKLADLRTAQ
                     G"
     gene            3920..5062
                     /locus_tag="VLA60_02270"
     CDS             3920..5062
                     /locus_tag="VLA60_02270"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HSF08207.1"
                     /translation="MSTRSLWREISLLFMFSLLGISAVLAETSSVGLHGFAPARPGYV
                     YAFPRDHGSHEQFQTEWWYFTGHLSTTNGRRFGYELTFFRRGIDSPDAWSNPSAWAMR
                     HLYFAHFALTDEANEQFQFAEKLSRAGINKAGAEGDRLHVWIDRWLLKTVAPDHRQFQ
                     LQAQAEDFSIDLTLESSKPPVIHGTDGVSRKGREAEATSHYYSMTRLQTTGSVVVQGV
                     PLAVNGVSWMDHEFGSADLSDGLVGWDWFSLQLENNYDIMAYGLRRADGTFDPASSGT
                     LVRPNGSSTSLSLDALKVSVQERWTSPASGARYPSQWTLSFPSEKVELNISPRMANQE
                     LVTRRSTAVTYWEGAVDVTGLWKGENIHGQGYVELTGYAEPYRPSR"
     gene            5105..6133
                     /locus_tag="VLA60_02275"
     CDS             5105..6133
                     /locus_tag="VLA60_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08208.1"
                     /translation="MSRMFVIWVVSMVLGLTACGQRSDTIVSIALHPTKPNIIYVATD
                     EAVYKSSDTGATWTRFAGELARTRVISLALDPQLSATVFAGTMGDGTYKSPDGGRTWH
                     PYNSGIQKGTISAIVNQIVFNPLGTEMVYAATTVGVFRSLDGGRHWTERMQGMTEVSF
                     VVTLAIDPQRPNVLYAGTTGGVYRTLNATESWEKASTGMVASDAKMASMALGVNGIVV
                     DPTNSDVVYAGTTDGLYKSTDQAEHWTKVGGSIQHAYISAIQLDPTNPSILYMATSDR
                     VQKSEDGGKTWQPKIQGLEAASIRSLQMSLLDPRTLYVGTNGGGLYRSTDAGESWSRL
                     PLTPASGD"
     gene            complement(6198..7142)
                     /locus_tag="VLA60_02280"
     CDS             complement(6198..7142)
                     /locus_tag="VLA60_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08209.1"
                     /translation="MILIACLSWWIFPQPARAESSAPGIHWGALDFPDQEPVLATGLS
                     IFRFTEFNGEGKRFNGIRETIGLNLITTSWTRHWSNSLEGWSTNLTFGIGPTRNQPSE
                     SLQNDFVHERLYDIPQVPVRAKRKETDFTITGSLTRWGEIPGQRRVLFLGGGGQTGSL
                     YQELFARGGFRRWSPVKTIEYLSGTHDGWVADMFRPLRLSGMVRAGRLSNGAAFHDLA
                     NHSFAAQGSLSYGWYDEKTFRPLFEVEIGATIDSGMFNGNQGNSLEERFWTVAIRAHP
                     FTFETWNDQLNGQDFGPTYGGKLMMDLSFLLPDSWKGQ"
     gene            complement(7351..7743)
                     /locus_tag="VLA60_02285"
     CDS             complement(7351..7743)
                     /locus_tag="VLA60_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08210.1"
                     /translation="MVNKRSPSLRKLVLIVDDEPAVRRTVRGALESSGIECAESENGA
                     EALAWLEENEVDLVLADYHMPIMSGLTLLERVHGTLNGRTPRVILLSGVIDEIHKHKA
                     MGLGAYAIIDKPCNFRELVEKVNEALDF"
     gene            complement(8440..8925)
                     /locus_tag="VLA60_02290"
     CDS             complement(8440..8925)
                     /locus_tag="VLA60_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08211.1"
                     /translation="MALRILSFFIEGVTWRVAKTPTGYCHRGLSTMPEWIPGIPSGVS
                     KDIVESTFQQFPSDDDSLQPHNITFKLTYRVGSSNIECFIFSSPDGDLFSVRIPPGTF
                     IALQEALPSQTIPRMRVARLAVQHAIASGMPGIELLPGSAIYESVKSQLSGSCLQKAQ
                     V"
     gene            9445..>10188
                     /locus_tag="VLA60_02295"
     CDS             9445..>10188
                     /locus_tag="VLA60_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08212.1"
                     /translation="MRNLIVLLGAFSISLGCAGGPNGNEAYKKEGFQVTQPSPGTKVV
                     VRGNHAAAVNQALGWLNDHQLLVVNRQVVQKEGVPQFASRNGTEGQAQALGGARKVGA
                     TLVVFVRVDETSLNSTIEPVSGGSPPMNKVVVDILGMNTGAGEMVFEGKAWSSDPLVV
                     SEGVIQDLTILALEQAWQKPASSPSAKQEVNAESPSELPTPQMAPVAVDSSSSPTTTQ
                     SATVLLDSPNPGAATRSEPGPPTEMAQEQV"
BASE COUNT         2585 a         2387 c         2764 g         2452 t
ORIGIN      
        1 attggacgcc ttctgggcgg ccccagggac ggacgatttc tatactgcgg gccttgtctg
       61 agcgcagcga gttggcccgc tccacatctt gcgtccgtcc tatcaaatgt ggccggactg
      121 ggcgtcattg gttttggcta cttttgccga accaaaagta gctcggctgc cgggccgaaa
      181 cccggcacct tctaaatacc aaggtcaaac cccaacacat gtgaggaaaa gaaaaatgtc
      241 aggcggatga aactctcaac aacagatttg aatccgttag gaagctaatg taccaaacgg
      301 atatggccgg taggagctag agcgaaggat cctcatcttc ggaagcttcc tgcaggattt
      361 gaaccagggt ctctacccgc ttcgtcatgg gattctgttg ctcccgttca taaatactca
      421 ataggtttcg gagcatgcgg gcaagaatca gtcgtgttgg actcttttcc aaaaactcag
      481 gagaatagtc caaaccggat gcggtaataa attgttcaca gtcttgttcc cgtaaaaatg
      541 ccccaccgtt aaaacaatcc acaaattgcg gaatcggctc tccttcaatc ttcaccagga
      601 aatgtcccgg cattccgacc ccggagaggg gcaaattcaa acggtttccc acaaacacgt
      661 agagggccga gagagagatg gggatccctt gccgggattc gatgacccga tgcagatagc
      721 tattgtcggg gtcatggtaa tggatgcgat tgccggaaaa tcccttgtct ttaaagagaa
      781 acgcgctgag gagtctggcg acttttccag gcgcagaacc ttcggaatgc catttcttgc
      841 gaaattcttc cgtgagctga tcgaggcact gctgataatg gggaatatcg acatccgggt
      901 aggcgatctt ggcgatgagg aacgctccct gttcccaatc catctgtcgg gagcaatggg
      961 taacgaacgt gctgaattcc tctttgagtt tctcaaaaag aatttcatct ttgacaaaag
     1021 cgacccggga ttccaaggcc gtatcatctg cgccggcttt ttccagaaac ggtagggctg
     1081 agggcccgat acggatgaat tcgtgctgga tcgttttggc gacctgttcg ttggggtcgg
     1141 acagcaaacg aagtagagct tgaatttttg tcttcgaagg tgagtgcaag aaagttcgtt
     1201 ccgggtaaat acgacaacgc ctcctcaaag aaaaagaggc gtcccataga ggagtttata
     1261 atgtgataaa aggccgttag ccgaccgagc gcctgaacgc gcgggcaccc ccatagagac
     1321 acagtgcatc aaagagggcg aggactccaa gggcgatagc tacctgaggg agcgcctcgc
     1381 tccaaccggt aagaatcaac ggccgaatgg catcaatgac ccaggtcatc ggattgaaaa
     1441 atgccagtga tttcatccaa cccggcattg ccgctaatgg tacgagcgcg gaactgagaa
     1501 agatcatcgg gagcgaaagg aagcccaaca ctgagaagaa atctccatga cttttcaccg
     1561 aaaatgccat ggccatggaa atggcggtga gtccgacacc aaacagcatg ccgatcacca
     1621 gaatgagtgc gatccctaat atcccggttt ccgcactcac cccgaataaa aaggccacgc
     1681 ccagaataac cagaatttgc atggcggtga tggccatcac aaataggaac cggctaagga
     1741 tcacggacga tcgtctgatg ggagtcgcca tcagtcgttc cagaaacccg ttttctttat
     1801 caaacagtaa gtcgacgcca ccggccagcc cgttattgag cacggtcatc acgacaacgc
     1861 cggctgcaag aaaactcatg taattcgggg cttgaacgac ctgtatatcg gctgctcgct
     1921 gaaacaggtt gccgaaaaag atgagccaaa acagcatggg ttggatcaac gtaaacaaca
     1981 tactgaactt ttcccggctc aatcgcttga cccatcgatt ggtcagggca agtacctctt
     2041 gaatttgctc attcatggaa aaacctttcg agaacagtga aaatctacag gataagatct
     2101 taggtcgtgg gttcagaggg ttggtctttg agggaccggc ctgtgtgtgc cacaaacacg
     2161 tcgtctagcc tcggccgatg atattcgatt ccatcgagga gacatcctac tcgatttgtg
     2221 gcctcgagaa tagcaggcaa tgccttctcg ggagcatcga cgcggatatc aagcccttgt
     2281 ggagtgggac gaatcgcacg cacgaagggc aaggctttta aggcggttga gagggtttca
     2341 ataacctgag gctgagggtt gcctagagtg agccagacgc catcgccacc caagctggcc
     2401 tttaaatcct ttggagagcc aatggcgcgg atttctcccc ggtcgatgat ggcaattcga
     2461 tcacaaagct ggtcggcttc atccaggtaa ttggtggtca tgacgatggt catgccctgc
     2521 tcgcgcaatt gccgcaggta ttgccagata ttgaggcggc tttgaacgtc aagcccgagg
     2581 gtgggttcgt ccaaaaagag taccttggga ttgggcaata aaccgcaggc aatatccaat
     2641 ttgcgtttca tcccaccgga atatcctttg gccacgcgat cggcatgttc ttccagatcg
     2701 accagcttca ggagcgatgc aatcctggtg agggcttcat tctttgggag atgataaaga
     2761 tccgcaagca acagcagatg ctcccgaccg gtaagaaaac ggtcgatagc ccgttcctgc
     2821 ggaacgtagc cgatcatccg tcgaatggta tcggcttctt tggcgacatc atgcccgaaa
     2881 atggttgcgg tcccggacgt gggactgagg gtcgtaatta aaattcgaag cgtggtactt
     2941 ttccctgccc cgttgggtcc aagaaggcca aaaatttctc ctttcatgac ggagagggaa
     3001 agattcttca ccgcagtcac ggtgtcgtag gttttcccca gccctttaac ctcaatggca
     3061 acggcatcag acatgtgtgt tatccttgtg ccgttcgaag atcggcaagt ttaaagcgga
     3121 ttagatcaat caggcggcga gcttgctgga tgagatggcc ggattcaagt aaaaattgtt
     3181 tttccacggg tgacaaatca agaccggctg aaaggttatg aacaagaatg gaatccgtga
     3241 gtttgccaga agcgatcacc tgacacaact catgagcctt tttccaggtc aaatattctt
     3301 gggtaatttg ctccagattg atgcgggttt cgatgtccag ggagggtgcg cccttttgcg
     3361 cattcggaat aattcgtgct tggcggtagg gcgttgagac ctcttgctca tcaaaggtac
     3421 atcgctgaag gccttgcaac acaatattaa accgaccgtc agccagtttg tgtgagctga
     3481 taattcgccc cacgcatccg atgggataaa tcggcggatc cgcctcatac tcgtcttccc
     3541 aaccgtcctt gagcaagacc atgccaatgc aatctccctg accggatgcg ttatgcacca
     3601 tttcccggta acggggttca aagatgtgta gtggcaaata ggtttcaggg aaaaacacga
     3661 cgttgggcag tggaaaaatt ggtacgctgt ccggtatcgg aaatttgccg aattcggctg
     3721 atggaaaatc tgaaggcata cggtacgata gcggagaatt ttcgggaaag tcaacgtaaa
     3781 atcgtgaccc aaaggagttc ggtaaactga gaaaatcctt gtgtgccaag ggatttgtcc
     3841 gttatgtaag gtcgtggtat aaacggaaca tgaagtggac gacggccttc acccacgcga
     3901 cagttttgca ggtctggagg tgagcacgag atctttgtgg agagagatct cgctcctgtt
     3961 tatgttttca ctgcttggga tttcggcggt ccttgcagag acctccagtg ttggacttca
     4021 cggatttgct cctgcccgcc ccggatacgt ctatgcgttc ccccgggatc atggcagtca
     4081 tgagcaattt cagacagagt ggtggtattt taccggacat ctgtccacca cgaatggccg
     4141 acgctttggg tacgaattga ccttttttcg tcgtggaatt gactcacccg atgcctggag
     4201 taatccttct gcatgggcca tgcgacacct gtactttgcc catttcgcgt taaccgacga
     4261 agcgaatgaa caattccagt ttgccgagaa gctgagtcgt gccggaatca ataaggccgg
     4321 agctgaaggg gatcggctcc atgtgtggat cgatcggtgg ttgctgaaga cggtggcgcc
     4381 cgatcatcga caatttcagc tacaggcgca ggctgaagat ttttccattg atttgacgct
     4441 cgaatcgagt aagccccccg ttattcatgg cacagacggg gtgagccgga aaggccggga
     4501 ggcagaggcc acctcccatt attattcaat gacccgtctt cagaccactg gatcggtggt
     4561 ggtacagggg gtgccgctgg cggtcaacgg cgtcagttgg atggatcatg agtttgggtc
     4621 tgcagacctt tcggacggtc tcgttggatg ggattggttc agcctgcaac ttgaaaataa
     4681 ttatgacatt atggcctatg ggcttcgccg tgcggatggg acctttgatc cggcatccag
     4741 tggaacattg gtgaggccaa atggatcctc gacatccctt tcccttgatg cgctcaaggt
     4801 aagcgttcaa gagcgttgga cgagtccggc cagtggtgca cgttacccta gtcaatggac
     4861 gttatctttt ccgtctgaaa aggtggagtt gaatatctct cccagaatgg ccaatcaaga
     4921 acttgtcacc aggcgaagta ccgctgtgac gtactgggaa ggagctgtag atgtcaccgg
     4981 gctttggaag ggagaaaata ttcatggcca gggctatgtc gaactgaccg gatatgccga
     5041 accgtaccgc ccatcacgat aatgtgtgat ttcttaagat ttggtaatga aaatcccttt
     5101 taaaatgtcc cgaatgtttg tgatctgggt tgtgtccatg gtgctcggat tgacggcctg
     5161 cggtcaacga agcgatacca ttgtgtccat tgccttacat cccaccaaac ccaacattat
     5221 atatgtggcc acggatgaag ccgtatacaa gtcttccgat acgggggcca catggaccag
     5281 attcgcaggc gaattagccc ggacccgagt gattagtctt gcccttgacc cccaattgtc
     5341 tgccaccgtg tttgcgggaa cgatgggcga tgggacctat aaaagcccgg atgggggacg
     5401 aacgtggcat ccctacaatt ccgggatcca gaaaggcacg atctcggcca ttgtcaatca
     5461 gattgtgttt aatcctttgg ggacggagat ggtttatgcg gcaacaaccg ttggggtttt
     5521 tcgaagtttg gatggcggtc gacattggac tgaacggatg caaggcatga cggaggtgag
     5581 ttttgtggtg accctggcaa ttgatcctca gcgtcccaac gtcttatatg cggggactac
     5641 gggaggtgtc tatcgcacgc tcaatgccac ggagagttgg gaaaaagcat ccacgggcat
     5701 ggtggcctca gatgccaaaa tggcttcaat ggcgttggga gtcaatggga tcgtggttga
     5761 tccaacgaat agtgacgtgg tgtatgcagg gaccacggat ggattatata aatcaacgga
     5821 tcaggccgaa cattggacaa aggtcggagg tagcattcaa cacgcctata tcagtgccat
     5881 tcaacttgat cccactaacc cgtcaattct gtatatggcg accagtgatc gggttcaaaa
     5941 aagtgaggac ggaggaaaaa cctggcaacc gaaaatacag ggattggagg cagccagtat
     6001 tcgtagtctt cagatgagcc tcctcgatcc ccgcactttg tatgtcggca cgaatggagg
     6061 agggctgtac cgaagtacag atgccggaga gtcctggagc cggttacctc ttactccggc
     6121 atctggtgac taggtggata ggagccacgg cgctttcact acaatttctg tttcagtggt
     6181 ggatgcagga gcgaaagtca ttgtcctttc caggaatcag gaagcaggaa cgagaggtcc
     6241 atcatgagtt tgcctccata cgttgggccg aagtcttgac cgtttaattg atcgttccat
     6301 gtttcgaaag taaatgggtg ggcacggatc gcaacggtcc aaaagcgttc ctccaaggag
     6361 tttccctggt ttccattaaa cattccggaa tcaatggtgg ccccgatttc gacttcaaac
     6421 aagggccgaa atgttttctc atcataccaa ccatagctga gggagccttg ggcggcaaat
     6481 gaatgattgg ctaaatcatg gaacgcagcg ccattggaaa gtcgcccggc cctcaccatt
     6541 ccggataatc ggagaggtct gaacatatct gcgacccagc catcatgtgt tccgctcaga
     6601 tattcgatgg ttttcaccgg tgaccagcgg cgaaaacccc cacgggcaaa aagttcctga
     6661 tagagacttc ccgtctgtcc cccgccgcca agaaacagta cccggcgttg tcccgggatt
     6721 tctccccatc gggtgagtga accggttatg gtaaaatcgg tctcttttcg tttcgctcta
     6781 accggaacct gagggatatc atacaggcgt tcatggacaa aatcgttttg gagagattct
     6841 gaaggttgat ttctcgtggg gccaattccg aaggtcagat tggtgctcca tccttcgaga
     6901 gaattggacc agtggcgagt ccaactggtg gtaatgagat ttaatccgat ggtttcccga
     6961 atgccattaa accgcttccc ttccccatta aattcagtaa agcgaaatat ggagagtccg
     7021 gtggcaagca ccggttcctg atcgggaaag tcgagcgcac cccaatgaat acctggcgcg
     7081 gaactttccg cccttgccgg ttgtggaaaa atccaccaac tcaaacaggc gatcaggatc
     7141 atggaaacac tcaaagatga atgaaatact cggcaagcca taggcaaaga tgggaagttt
     7201 aatattgtcg agcggtagtt gacttgtttc tagcacaagg gaatgcctta gaacatttgc
     7261 cgtattgtgg gggatgaccc tacagcaggc ttactctcga gttgtctagg cggataggac
     7321 ctttagcgag atcaaccggg aggggggaat tcagaaatcg agagcctcgt ttactttttc
     7381 gacgagttca cggaaattac agggtttgtc aataatcgca taggcgccaa gtcccatggc
     7441 tttgtgcttg tgtatttcgt caatgactcc actgagcagg atgacccgag gggttctgcc
     7501 gttcaaagtt ccgtggactc gttccaacaa ggtcagtcca ctcataattg gcatgtgata
     7561 gtcagccagg acgagatcga cttcgttttc ttctaaccac gcgagtgctt cggcgccgtt
     7621 ttccgactcg gcgcattcaa ttccggagga ttctaaggct ccccgtacag ttcggcggac
     7681 ggcaggctca tcatccacaa tcagcacaag ttttctaagg gaaggagaac gtttgttcac
     7741 cacgatgtgt tcctcggaaa caccataaga acattcaact ttttctcgga tcagttgttc
     7801 gttctttagg gcaaatgtac cctattgttg catacgtaat gtgacttggg gaataccctc
     7861 accgtatatc ttgtgttatt tttttacaac agcacaatgt ttgaatattt gacaaaatga
     7921 tgaaagtatt atttgtccat agcgtataac aatttatgca tagctgacca acaggattgt
     7981 gcacagcttt ggcgggtaaa aagggggaat cgacactatg aatgatttat gacggaaaag
     8041 aatgaggtaa cacttcggag cgttgggaga ttcagaaggt agattgtgcc ggatagcagg
     8101 attttcaggg acttgccacc tggttcatcg aaaggggctg ttgaaaaaag ccgccagcgg
     8161 cgttccctgc cttcgccgga gcggcttcgc gcaggcaggt cgccattttt ccgtgctcac
     8221 gtactggaag tacgctctgc gcgcaaaaat gactgcggcc ttgctggacg aacttttttg
     8281 aaccgccccg aggcccctga tttgcaacgt gcctgtgggt caaaatgctc ctggaaatta
     8341 gtattattca acattccccg aaatattttt caaccggtat ccctgaataa tggccttgaa
     8401 tggagacagg acgggtcagt tcgatagaaa gcgaaaggct tacacctggg ctttttgcag
     8461 acaggatcct gacaattgcg atttgactga ttcgtatata gcacttcctg gaagaagttc
     8521 tatccctggc atccctgacg caatcgcatg ctgcacggct aaacgggcga ctctcatgcg
     8581 tgggatagtc tgggatggaa gagcttcttg aagagcgatg aaagtaccag gaggaatcct
     8641 gacagaaaag aggtcgccgt caggcgagga aaagataaag cattcaatat tggaggaccc
     8701 gacacgatag gtcaatttga atgtgatgtt gtggggctgg agggagtcat catcagaggg
     8761 aaattgctga aaggtgcttt ccacaatatc cttggatacg cccgagggaa ttcccggaat
     8821 ccactctggc atggttgaaa ggccacggtg acagtagccc gtaggggttt tcgccactcg
     8881 ccaggtgacg ccttcgatga aaaatgacag aattcgtaaa gccatgaaaa tttttaaaaa
     8941 taaatttgag cgtaaatcgt ttatgcaggt ctatcggccg aattacttaa ttcattagca
     9001 tactagggaa gctggggctc acgcaattct cgtcatgaca gagtaatgat aaaagataaa
     9061 gtgtttggtg aaagatgagt cgaaaggtta aaaaccattc tcatttttcg gttaaggacg
     9121 ttcccgggat aggaagatct ccaacaagga tttttctcta ttctatcaga aaagaaagaa
     9181 attaacgggt ctctctccgt gaaggctcaa tttgtggtat tttaattttt caatacggtc
     9241 gtcatatgct aatttttcat ttgaaaccgg accgtacacg caaaatcctc cgattctcat
     9301 gtgaatgttg gacaggaagc ccggaccacg taattggtga gctggattat tggaaaactt
     9361 ctttgggggc ttacaaacgc acatctttac aatagaaatg gtaatgaagg gaacatcact
     9421 atgtcggatc taggtggtcg gcacatgaga aacctgatcg ttctgcttgg agcgtttagt
     9481 atttcgttgg gatgcgctgg tggtcccaac gggaatgaag cctataaaaa ggaagggttt
     9541 caggttacac aaccaagccc gggaacgaag gtagtggtgc gcgggaatca tgcggcggcc
     9601 gtcaaccagg ctttggggtg gttgaatgac catcaattac tggtggtcaa tcgacaggtt
     9661 gtgcaaaagg agggagttcc tcaatttgct tctcgaaatg ggacggaagg gcaagctcag
     9721 gctctagggg gagctcgtaa ggttggggcg acattggtgg tatttgtgcg cgtagacgaa
     9781 acttccttga attccacaat agagcccgta agtggtggat ctccacctat gaataaggtc
     9841 gtcgttgata ttcttggaat gaataccggt gcaggcgaga tggtctttga aggcaaggcc
     9901 tggagttctg acccactggt ggtgtctgag ggggtgatac aagatctgac cattctggcc
     9961 cttgaacagg cttggcagaa accagcgtcc tccccctccg cgaaacagga ggtgaacgct
    10021 gagagcccga gcgagcttcc tactcctcag atggcgccgg tagcggttga ttcgtccagt
    10081 tcacctacca caactcagtc ggcgacggtc ctattggatt cgcctaaccc aggtgctgct
    10141 acccggtcag agccggggcc gccaaccgag atggcacaag agcaggtg
//
LOCUS       DATIAL010000070         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_442429, whole genome shotgun sequence.
ACCESSION   DATIAL010000070 DATIAL010000000
VERSION     DATIAL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442429"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /gene="hflX"
                     /locus_tag="VLA60_02300"
     CDS             complement(<1..1493)
                     /gene="hflX"
                     /locus_tag="VLA60_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSF08213.1"
                     /translation="MERLYRRRIPPEKVVTPEVARECAELTYETRRQIGLLVNRQGQI
                     ESVLIGDHHELVIPHLSRTRSGLRLLRGVRLVHTHLNNQPLTQDDLTDLALLRLDLIM
                     ALGVGKDGSLRDVYVAHILSNPIQGRTTVELPPCAFHSFQLDCQQFIQSLEQEIVRSN
                     PGLREGVREGRALLVSVSTGNAASEEGRLQELQELVRAQNVEVLGCITQRLGAIHPKF
                     VVGSGKMKELAIRALQSGADTLIFDQDLTPAQVRGISEITDLRVLDRSQVILDIFAQR
                     AHSSIGKIQVELAQLRYLLPRLAQSSTALSRLAGGIGTRGPGETKLETDRRRARERIQ
                     RLERDLESMAHGRQEQRKMRMKRGLPIISIIGYTNVGKSTLLNALTKSQVSVKDRVFE
                     TLDTVNRRWYLPGIGDVLLTDTVGFIRDLPKDLMAAFQTTLLELQEADVLLHVIDAHV
                     PDPGQHIIAVDNILKELGLENIPCLRFFNKADLMREEDVQLLCQRYGG"
     gene            complement(1578..2618)
                     /gene="tsaD"
                     /locus_tag="VLA60_02305"
     CDS             complement(1578..2618)
                     /gene="tsaD"
                     /locus_tag="VLA60_02305"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSF08214.1"
                     /translation="MILGIETSCDETAVAVLDGDGHILSNILDSQVEVHARYGGVVPE
                     LASRRHMECLELLTREALGLANVSLSQLTGIAVTNRPGLVGALLVGVNFAKALAFATK
                     IPLVTVNHLEGHLASAWLSNPNFPTPAMVLIASGGHTHLALIPHRGEYQFIGWTMDDA
                     AGEAFDKGAKMLGLPYPGGPAIDKLAQQGNAQYVSFPRPTLQSQNFNFSFSGLKTALR
                     YFVRDEQQKGNSPLPVADIAASYQEAIVDVLVEKLCRAARHYEVKGISVVGGVAANSR
                     LRLRLEEGARTLGLHVTLPPRALCTDNGAMIAAAGLEKLKRKERAAWDVDAISTLQCK
                     LDGFAMAGLPSQ"
     gene            complement(2726..>5150)
                     /locus_tag="VLA60_02310"
     CDS             complement(2726..>5150)
                     /locus_tag="VLA60_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HSF08215.1"
                     /translation="ERFTDRGRKIIILAREEAERHQNDYLGTEHLVLAILRESDGIAL
                     MIIKKMGLSPEQIRLEIERNLPGGGTTVTFGEIPFSPRVKKVIEYGVEEARLLGHNHI
                     GSEHLLLGLLREEEGIGGKVLRSLGANLLTARQLTVTFLRKSGTRERDKKSSTPALDE
                     FGRDLTQLAQEGTLDPVIGRSDEIQRVLQILSRRTKNNPALIGESGVGKTAIVEGLAQ
                     RIIGLDVPDNLLNRRVIALDLGSLVAGTKYRGQFEERLKVVMKEISQAGNIILFIDEL
                     HTLVGAGAAEGSIDAANMLKPALSRGEIQCIGATTLDEYRKHIEKDGALKRRFQPIYV
                     QPPSVEETVQIIQGLRDRYEEHHGVEITDEAVEEAVKLSERYITDRFLPDKAIDLIDE
                     TGSRAKLLTYALPPELKSLEQELKRVAREKEVAIGLQNFEEAVKFREEEERFRKLLDD
                     SKRDWKKKQEKNKPVISGEDVAYVISKMTGIPLFKLEEEESEKLLHMEDYLHKRIIGQ
                     EEAISAVCRSIRRSRAGLKDAKKPIGSFIFLGPTGVGKTELARALAEFLFNTDDALIR
                     VDMSEYQEKFTSSRLFGAPPGYVGYEEGGQLTEKVRRRPYSVVLFDEIEKAHPDIFNV
                     LLQVLDDGVLTDSLGRKVDFKNTILVMTSNLGTRFIQKGVSLGFQRDVEAEHSSRMRG
                     EVLAELKRHFSPEFLNRIDELVVFHQLTKEHLVSIVDILIGELNARLVERGVFLDVTD
                     EVKQWLIDEGFEQQYGARPLRRTIQRRLGDPLSEELIKGRFKDAKKIKVVLTDQTPNF
                     IEEEVLASV"
BASE COUNT         1343 a         1412 c         1073 g         1322 t
ORIGIN      
        1 cctccatatc gctgacagag gagttgcaca tcttcttccc tcatcagatc ggctttatta
       61 aaaaaacgca ggcatggaat gttttcaagc cccaattcct tcaatatatt gtctaccgca
      121 ataatatgct gtccaggatc ggggacatgg gcatcgatca cgtggagcaa cacatcggcc
      181 tcttgcaatt ccagcaaggt ggtctgaaat gccgccatga ggtcttttgg caaatcccgg
      241 atgaatccta cggtatctgt cagaaggacg tcgcctattc caggtaaata ccagcggcga
      301 ttgaccgtat caagtgtctc aaaaacacga tcttttacag agacctggct ctttgtcaac
      361 gcatttaaca gagtcgactt ccccacattg gtatagccaa taatggaaat gataggaagg
      421 cctcgcttca tgcgcatttt tcgttgttcc tgacgaccat gtgccataga ctccaagtcc
      481 cgctccaatc gttgaatgcg ctcgcgggct cgacgtcgat ccgtttccaa cttggtttct
      541 ccaggccctc gtgtgccaat tcctcccgct aaccgggaaa gagccgtact ggattgagca
      601 agtcgtggga gcagataccg cagctgggcc agttcaactt gaatttttcc aatggaggaa
      661 tgggcccgct gtgcaaaaat gtcgagaatc acttgtgagc ggtccaagac ccgcaggtcc
      721 gtgatttccg aaataccccg tacctgagcg ggcgttaaat cttggtcaaa aatcaaggta
      781 tcggcaccgg attggagagc tcgaatcgcc agttctttca tctttcccga accaacgaca
      841 aacttcggat gaatcgcacc caaccgttga gtgatacacc ctaaaacttc cacgttctga
      901 gcacgtacca attcttgtaa ttcctggaga cgaccttctt ccgaagcggc attccctgtg
      961 ctgacactaa ccaggagcgc cctcccctcc cgaacacctt ctcgtaaccc tggattcgag
     1021 cgaacaattt cttgctccaa tgattgaata aattgctggc aatccaactg aaacgagtga
     1081 aaggcacaag gcggtaactc caccgtggtt cgtccttgaa tggggttcga aaggatgtgc
     1141 gccacataga catcccgaag gctcccatcc tttcccacac cgagcgccat aattaaatcc
     1201 aatcgaagaa gagccagatc ggtcagatca tcctgggtca ggggttgatt attgaggtgg
     1261 gtatggacta aacgaacccc tcgcaataat cgaaggcctg accgggtccg cgaaagatgg
     1321 ggaataacca attcgtggtg gtcaccgatt aaaaccgatt caatttggcc ctgtcgattg
     1381 accaaaagcc cgatttgccg tctcgtctca taggtgagtt cggcacattc ccgagccacc
     1441 tcaggggtca caactttttc tggcgggatc cgtcgacgat agagacgctc cattcggcta
     1501 atgtgattag cctttaaccc atgaagatta ccgagaacaa caggaatgat tcttctcctt
     1561 ttgcgttatc atctgagtca ttgagaagga agtccagcca tcgcaaaccc atccaattta
     1621 cattgcaacg tagagatggc atctacatcc caggctgctc tctctttgcg cttaagcttt
     1681 tccaaccccg cagctgcaat catggcacca ttgtccgtgc agagcgcgcg tggaggtagc
     1741 gtcacatgca gccccaatgt gcgagcaccc tcctcaagcc tcaggcgaag gcgggagttt
     1801 gcggccactc ctcccacgac agagatccct ttcacttcat agtgacgggc agcgcgacaa
     1861 agcttctcca ccaacacatc cacaatcgct tcctggtaac tggccgcgat atcagcgacg
     1921 ggcaatggag aattcccctt ttgttgctca tcccgcacga aatatcgaag tgcggttttc
     1981 agtccactga aactgaaatt aaaattttga ctttgaagag ttgggcgggg gaatgataca
     2041 tattgtgcat tcccctgttg ggcaagcttg tcaattgccg gaccacccgg atacggaagc
     2101 ccgagcattt tggcgccttt atcgaacgct tctcccgctg catcatccat cgtccatcca
     2161 atgaactgat attctccacg atgaggaatc aatgccaaat gcgtgtgccc accagacgca
     2221 atcaagacca tggccggggt tgggaaattt ggattcgaca accatgcgga cgccaaatgc
     2281 ccctccaaat gatttactgt caccaaagga attttggtgg caaacgccag agctttggca
     2341 aaatttactc caacgagtaa ggcccctacc aatcctggac gattggtgac cgctatcccc
     2401 gttaattggg aaagagaaac attggccaga cccaaagctt cccttgtcaa aagttccaga
     2461 cactccatat gacgacgcga agccaattcc gggacgactc ccccgtaacg ggcatgcacc
     2521 tcaacctgag aatccaaaat atttgaaagg atgtgaccgt cgccatccaa cacagccacg
     2581 gccgtttcat cacaggaagt ttcaattcct aaaatcactt aagagtcctt tccagcacca
     2641 aaattcattt ggcaaaaaac aacaaaaccc ccaggactcg cggtcctgag ggtttcatct
     2701 aggatttgac catccctata tccaatcaga cactcgctaa cacctcttct tcaatgaaat
     2761 ttggagtctg atccgttagg acaaccttaa tctttttggc atccttaaac cgccccttaa
     2821 tcagttcttc agagagagga tctcccagtc ggcgttgaat agttcggcga aggggacggg
     2881 caccgtattg ttgttcaaac ccttcgtcaa tcaaccactg tttcacctcg tcagtgacat
     2941 ctaaaaatac tcctctttcc acaagacgag cattgagttc tccaataaga atatcaacga
     3001 tactcaccaa atgttctttc gtcagttggt ggaagacaac taattcatcg atacgattta
     3061 agaactcagg actgaaatga cgctttaact cggcaagaac ttcgcctcgc atacgtgaag
     3121 aatgctcagc ctctacatca cgctggaacc caagggatac acccttttga atgaatcgag
     3181 tccccaaatt tgaggtcatt accaaaatgg tatttttaaa gtccaccttc cgacctaaac
     3241 tatccgtcaa tactccatca tcaagaacct gcagcaacac attaaaaata tctggatggg
     3301 ccttttcaat ttcatcaaat aagacaactg aatagggacg acgtcttacc ttctccgtca
     3361 attggccgcc ttcctcatac cccacgtaac cgggaggggc tccaaacagc cgtgaactgg
     3421 taaatttctc ctgatactct gacatgtcta ctcggattaa cgcatcatca gtattgaaca
     3481 agaattctgc taatgcgcga gccaactccg tcttacctac cccggtcggt ccaaggaaaa
     3541 taaacgaccc tatgggtttt ttggcatctt tcaaaccggc ccgcgaccga cgaatggacc
     3601 gacagactgc agaaatggct tcttcctgtc ctataatacg tttgtgaagg taatcctcca
     3661 tgtgcaacaa tttttcagat tcttcttctt caagtttaaa gagaggaatc ccagtcattt
     3721 tcgaaatgac atacgccaca tcctcgcctg agataaccgg tttatttttc tcttgctttt
     3781 tcttccaatc acgcttggaa tcatctaata atttgcggaa tcgctcttcc tcttcgcgaa
     3841 atttcacggc ttcctcaaaa ttttgaagcc cgattgccac ttctttttca cgagcgactc
     3901 ttttgagttc ctgttccagc gatttcaact caggggggag agcatatgtt agcaactttg
     3961 ctcgcgaacc ggtttcatcg ataaggtcaa tagccttatc aggtaaaaac ctgtcggtaa
     4021 tatatcgttc actcagcttc acagcctctt caaccgcctc atccgtaatt tcaactccat
     4081 gatgttcttc atatcgatca cgaagcccct gtatgatttg cacagtctcc tctactgaag
     4141 gaggctgaac ataaattggc tgaaacctcc gcttgagtgc cccatccttt tcaatatgtt
     4201 ttcgatattc atccagggtc gttgcaccaa tgcattgaat ttccccacgg gacaaggccg
     4261 gctttaacat attcgcagca tcaatggacc cttcagcagc accagcaccg actaacgtgt
     4321 gaagttcatc aataaaaaga ataatattcc cagcttggga aatttctttc atcaccacct
     4381 taagacgttc ttcaaattgt ccccgatatt tggttcctgc taccaaggaa ccaagatcca
     4441 aggcaatgac cctgcgattc aataaattgt ctgggacatc cagcccaatg atccgctgag
     4501 ccagcccttc cacaatggct gttttcccca cgccggattc accaattaat gccgggttat
     4561 ttttcgttcg acggctgaga atttgaagaa ccctttgaat ttcatctgaa cgtccaataa
     4621 cagggtctag cgttccctcc tgagccaatt gcgtcaaatc cctgccaaat tcatccaacg
     4681 caggtgtgct acttttttta tcccgctccc tggttcctga ttttcgaagg aatgtgacgg
     4741 tcaattgacg agccgttaaa agattggccc ctaaactacg aaggactttc cccccaatgc
     4801 cttcttcctc acgaagaagg ccaagaagca gatgttcgct cccaatatga ttgtgtccca
     4861 gcagtctggc ctcctcgacg ccatactcaa tcactttttt cactctggga ctaaacggga
     4921 tttcaccgaa cgtaaccgtt gtaccacctc cgggcagatt ccgttctatc tcaaggcgga
     4981 tttgttccgg cgagagaccc atttttttga tgatcatcaa ggctatgccg tctgactctc
     5041 gaagaattgc gagaaccaga tgctcggtac ccagataatc gttttggtgc cgttccgctt
     5101 cttctctggc aaggataatg attttcctac cacgatccgt gaatcgttcg
//
LOCUS       DATIAL010000071         6758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_657403, whole genome shotgun sequence.
ACCESSION   DATIAL010000071 DATIAL010000000
VERSION     DATIAL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657403"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /gene="rph"
                     /locus_tag="VLA60_02315"
     CDS             complement(<1..542)
                     /gene="rph"
                     /locus_tag="VLA60_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HSF08216.1"
                     /translation="MMLPTLLTPRSDNRRVDELRPVKITRPFTKYAEGSVLIEMGETK
                     VVCTASVEEKVPPFLRDKGQGWITAEYGMLPRSTHDRMSREASKGKQGGRTLEIQRLV
                     GRSLRAVTDMHQMGERSIWIDCDVIQADGGTRTASITGAYIALADAFSTLKEKGLIKQ
                     CPLLDYLAAISIGKIAGEIR"
     gene            904..2187
                     /gene="serS"
                     /locus_tag="VLA60_02320"
     CDS             904..2187
                     /gene="serS"
                     /locus_tag="VLA60_02320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HSF08217.1"
                     /translation="MHDIRILSDQLDSLRAQLGARAGDIPWETVQTLAAQRRVLITQT
                     EELRHQLKKGSDQIAELKRNKQPCEDATTALREIRDRIQTMDVELRTTEEQLQDQALR
                     IPNIPHESVPPGKDEHENVEVRKWGIPPEFSFPAKSHQDLGEALGILDFKRATKIAGA
                     RFCVSMGLGALLERALANFMLDCHIQVHGYTEVLPPMLVNRPTMTGTGQLPKFAEDLF
                     HLPEEDFFLIPTAEVPVTNLFREEILEAEQLPLRLVAHTSCFRREAGSYGKDTQGLIR
                     MHQFQKVELVNFVRPEDSYDQLEHLTQAAESILQKLGLPYRVVALCSGDLGFSAAKTY
                     DLEVWLPSQQRYREISSCSNFDAFQARRANIRFRSKKDKPQFLHTLNGSGLAIGRTVV
                     AILENYQQADGTVHIPEALQPYMKGVDVIRPVAFP"
     gene            2459..2546
                     /locus_tag="VLA60_02325"
     tRNA            2459..2546
                     /locus_tag="VLA60_02325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2493..2495,aa:Ser,seq:tga)
     gene            complement(3798..3980)
                     /locus_tag="VLA60_02330"
     CDS             complement(3798..3980)
                     /locus_tag="VLA60_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08218.1"
                     /translation="MGANKFTPGRGFFSLGGRWDVLTLQKIADRLVARHIADILQSSN
                     NAVVFPRAILFGKLPD"
     gene            complement(5361..5768)
                     /locus_tag="VLA60_02335"
     CDS             complement(5361..5768)
                     /locus_tag="VLA60_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006170457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSF08219.1"
                     /translation="MYRKSNVNQLAFEDFVLPFGGKLRSDNRWVILAKQIPWADVEIA
                     YEPQFSQEDLCSPAKSSRMAPGALILKERLGVTDRELVEQIAENPYLQYFLGLMAYQD
                     EVPFHHSLLTTFRKRFTHDSLGAINEAIARPIS"
     gene            6269..>6758
                     /locus_tag="VLA60_02340"
     CDS             6269..>6758
                     /locus_tag="VLA60_02340"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HSF08220.1"
                     /translation="MSKTLPSWDLQGLLRHPTKDFKRITKKLDSLISELEVARPQLSP
                     DISAARFKKIWEQYETVTEYMTTLRAFSFLWFSENTKNQEARAFDTQVRNRLTDCSNR
                     LVFLDLWWQSLDPTNAARLTAKAERFRYHLETLTRFTPHTLSEPEERILTIKSTTGRQ
                     ALE"
BASE COUNT         1782 a         1774 c         1609 g         1593 t
ORIGIN      
        1 aaccggattt ccccggcaat ctttccaata ctgatggccg ccagataatc cagaagcggg
       61 cattgtttga tcaaaccttt ttctttcaag gtggaaaagg catcagccag ggcaatatag
      121 gcaccagtga tcgaggcggt ccgtgttccc ccatccgctt gaatcacatc acaatcaatc
      181 caaatggagc gttctcccat ttgatgcata tccgtgacag cccggagcga tcgtcccacc
      241 agccgttgga tttccagggt tcgtccaccc tgcttgcctt tgctggcttc acgggacatc
      301 cggtcatggg ttgaacgggg caacatccca tactcagcgg taatccagcc ttgtcctttg
      361 tcccgtaaaa aaggcggaac tttttcttcc accgaggccg tacacaccac cttggtctct
      421 cccatttcga tgagcaccga tccttcggca tatttggtaa atgggcgagt aattttgaca
      481 ggccggagtt catccacccg tcgattatct gaacgcgggg ttagcagtgt cggtaacatc
      541 attcctcaca ttaaaaaaag ggttgaatta taaagagggc tcctgcaaaa cacaaataca
      601 ccagacacct tgtccatatg aggttgaaag aatggctgat caatttagat ggagatgaca
      661 cacaatcttc gtcgttaatt ttgagagccg gatgaatcat tttttacgat cctggacaac
      721 ataccagcga aagaatagca ctcccgcaac gatcaaactc accaggacaa caaacacgtc
      781 ttcatgggga aagagataag gcatggatgc taggctgcgg tcgaaatgac aagggttttc
      841 cggtttgcat tcattgactg agatggggac tcatgataga ttcgggttcc ctgccaggcc
      901 accatgcacg atatccgaat ccttagcgac caactcgact ctctccgcgc ccaactcggt
      961 gcccgggctg gcgatattcc ctgggaaacc gtccagacct tagcggccca acgacgcgta
     1021 ctcatcactc aaaccgaaga gcttcgccac cagctcaaaa aaggatcgga tcagattgcc
     1081 gaactgaagc gtaacaaaca accgtgcgag gatgccacaa ccgctcttcg cgaaatccgc
     1141 gaccgcatcc agaccatgga cgtggaatta cgcaccaccg aagaacaatt acaagaccag
     1201 gccctcagga tccccaatat tcctcacgaa tcagttccgc cggggaagga cgaacacgag
     1261 aacgttgaag tgagaaaatg gggaattcca ccggaatttt cctttccggc caaatcccat
     1321 caggacctgg gagaagccct cggcatactc gatttcaaac gggccacaaa aatcgccgga
     1381 gccagatttt gcgtgtcgat gggcctgggg gcgttattgg aaagagcgct ggccaatttc
     1441 atgcttgatt gccacatcca ggtacatggc tatacggaag tgcttccccc catgctcgtc
     1501 aatcgaccca cgatgaccgg caccggccag ttacctaaat tcgccgaaga tctctttcat
     1561 ctacctgaag aagatttctt tctcattccc acggcggaag ttcccgtcac caatttattt
     1621 cgagaagaaa tcctggaggc agagcaactc cccttacgcc ttgtggccca tacctcctgt
     1681 tttcgacggg aggccggatc ctatggtaag gacacccaag gcctgattcg catgcaccaa
     1741 ttccaaaaag tggaactggt caactttgtt cgtccggagg actcctatga ccagctggaa
     1801 cacctgaccc aagccgcgga atccattctt caaaaattgg gactccccta tcgtgtcgtc
     1861 gcattatgct ccggtgacct gggattttcc gccgcgaaga catatgatct tgaagtctgg
     1921 cttccttcgc aacaacggta tcgggagatt tcgtcctgta gtaattttga cgcgtttcaa
     1981 gcccgtcgag ccaatattcg attccgctcc aaaaaggaca agcctcaatt cctccataca
     2041 ctcaatggct caggcctggc cattggacga acagtggtcg ccattttgga aaactatcag
     2101 caagccgatg ggaccgtgca tatccctgaa gccttgcaac cctatatgaa gggagtcgac
     2161 gtcatccgtc cggttgcttt tccataagtg aaaaattgat gaaccctatc cccatgtggg
     2221 atcttcgagc tcctggatga cacaaatttt tagcggactc ccaccaaatt tcattaagcc
     2281 agagagttca cattcctgct acagtcggct actataagct tgaatggaga tgagccttgt
     2341 gagattcatt gcatcccgta tcaaacggaa ggtcctgtca ggtcattgga gttagggccg
     2401 caactgggtg agaatcaatc ctaccaacaa tcctcacgct tttgagtcta aaaaattcgg
     2461 aggggtgcta gagtggtcga atagaccggt cttgaaaacc ggcgtcgtga aagcgaccgt
     2521 gggttcgaat cccaccccct ccgccagcca tagcaagagc tacggctggc aagccagcac
     2581 agaagagagc caagatttca ccaagtaaat taccgaagta aagagggcga aggctgtccg
     2641 ccgaagtctt gcagccaacg atcgatactc agcttacttt cactttagaa gtgggtactt
     2701 caatagcaag caggatatag tgacgaaggc ggacttacct cgttcctctt ctctatgaaa
     2761 tatgtctatc tgatcaaaag cgaatcacat cctaagcaga gttacatcgg tatcacttcc
     2821 gatttaaaaa accgactggc cattcacaac ggtggcggat ctgtccatac gtcgaagttc
     2881 aagccttgga agctcataac ctttatggct ttctctgaag actcaaaagc ccttgcgttc
     2941 gaacgctacc tcaaaaccgg gtcaggaagg gcattcgcca agaaaagact gtggtaagtg
     3001 gggtttttcc ccttcatggg gtgaccgtag cctggcgaag gcacgagggg gcatggcccc
     3061 cttgtgggag tgcggggcta gccaggcccg cacgacttcg aattcccacc ccctccgcca
     3121 gctagtattt acaggagaaa tgtcatctct ctgcgttttg acatcgctaa ctcccagctt
     3181 cttttcgcga tgagattggt cattttattt ctcccgattt tcatcaccct gagcctttcg
     3241 tttcctctgc cagccaaatg ggcctcgttc ggtagaattg gtaacaacct ttcgcgaggt
     3301 taggaacacg agtggcaccg tgagttcgaa ttcccgtatg gttaaaaaaa tcgaaccctg
     3361 tgtttcatga ggatttacct cacaccctca gcactcccct cccacttgtc tctcccctcc
     3421 ccgatccccg atactccacc accccaagat gaccctcatc atccccgcgt ggaggacgga
     3481 gtggaaaacg ttactcgcct atattaacgg aacggtagat atagaaatct tggtgcggag
     3541 tgaatatctc gtggctgaaa accgcatttt gcggaaccag atccgggaaa gatgcggccc
     3601 acagaaggcg agcggaccac gctgacggca cttaataaac gcctggggaa acaggccctg
     3661 gcggacgtga tgtcggtggt caaaactgaa acgttgctgg cctggcactg gagactgatc
     3721 gcaaagaagt tcgatggctc tcctctcagc atcggcctaa tctaggaagg ccacgttgcc
     3781 atcaagaggt tgaggactta gtcaggcaat ttgccaaaga gaatcgcacg tgggaatacg
     3841 accgcattgt tggagctttg aagaatgtca gctatatggc gagcgaccag acggtcggca
     3901 atcttttgaa gcgtcaggac atcccatcgg cccccaagcg aaaaaaatcc acgacctggc
     3961 gtgaatttat tcgctcccac cagaacctcc tcgtcgccac caatttcttt accacagaag
     4021 ttttgacgag gagtggaatg gtgacctatt acatcctatt ctttgatatg aagtcggagg
     4081 gtactgccgg cctgactcca gcagtatttg gcccacgatt ttgaggggaa attctgcccc
     4141 gcctttccgg agttggtcaa agcggctgga ctcaccctca tcaaactgtc cccacgcaat
     4201 cctaatttga atgcctactg agagatgggt gaggtttgtg aaggaggaag tcttgtcgcg
     4261 gctgattctg cttggagaag gcactctcag gaaagtgcta aacgaatacg gcacgcatta
     4321 acatcaggaa cgcaatcatc aaggaaaaag gaatggtctc ttgttgacac tggccagtca
     4381 aggcactcag cacgtaactt gatacgctct cgagaatgac taggtggttt gctgaaagca
     4441 attctaccga aagaccgcat gagttttttg accatacggc caggctctcg aggaaaacat
     4501 caactaatca caggaaaata tggcactgat aatcttaaaa gggatcgtcc ccaaaggttg
     4561 aagctaggac ctcagattta ccatccgact tttgaggacg ttttaataag agataaggcc
     4621 catttggaat catacatacc gcctcccaac ctttgtttcc tagaagaaga agggccgcct
     4681 caatttcaac agaatcgcct gaccgcacct tgtggcagca atattcccat gcaatcatac
     4741 aattcccctc ctatggttga ttattcctac acagaatgtc caacagtaga atccacggca
     4801 ttgaggtact ctaatcgttt ttcgaataga tttattacaa acagatgatt tgcgcaacaa
     4861 ttctgtcact ggatttcacc aaattccgtt ttttatctta agggaatttc taaaaaccct
     4921 gacgttggcc gcaatcgtct cggttgctcg cagagacgtt tttcaagtgg ggttggcgac
     4981 agggcaagcg ggaatctgag acgcacttgt cggcctgtcc tcacctctgt tccgctctct
     5041 tacacctcat aaacggttcg aggaattgtg agaatcagaa gccgttggaa gttgtccgtc
     5101 aaattcttca gcccgatcgt tgccctggct cacgcgaggc caatcgtgct aatcagttta
     5161 atcccatcgc ggtgacctag tgaccgaaca atcgaacaca tgttgacccg ggcgcgaatc
     5221 cgcgaacggg ttcgattgca cgtgggctgt tgagccgtca aggccttatg gcgaagcaag
     5281 tgactgtatg gcataaattt ttcgtcttga ttttttaata aatccaaagg ggaaaaagtc
     5341 aagatatcgc cccgggcagt tcatgaaatc ggccgggcaa tggcctcgtt gattgctcct
     5401 aagctgtcgt gggtgaaccg tttgcggaag gtggtcagca gagaatgatg aaacggtacc
     5461 tcgtcttgat acgccatgag gcccaaaaag tattgcagat aagggttctc cgcaatctgt
     5521 tccaccagtt ctcgatccgt gacacccagg cgttctttca gaatgagggc tccaggggcc
     5581 atgcgtgacg acttggcagg cgagcacagg tcctcttggg aaaactgggg ttcgtaggcg
     5641 atctccacgt cagcccaagg aatctgtttc gccaaaatca cccagcgatt atcgctgcgg
     5701 agtttgccgc caaaaggcag cacgaaatct tcaaaggcca actgattcac atttgatttc
     5761 cgatacatga ggcccctggt gcaagctttt tggtgggaga acggccatat ctgtgcacgt
     5821 cgcccttggt tgtacaagca aaagcccgct cgaatcaact cattaggctt ttttcagcaa
     5881 gccctaatta cctgtatttc ccctgttaag gacggaatcg gagattggag ggtaatgact
     5941 acccgattag ggatgagtca gggacaaaca atggatggaa tgagtaaact ctatagtagt
     6001 aggaataagt tcgctgctga ctgcccactc aggcactatt ccacaagcac agaatctatt
     6061 taatgattgg agaatattgc accaaaatta gggcaaagaa tgaaaagaaa attcaaacat
     6121 gatttggggg ccgcccagac gaattagaaa aaattgagga taaggccgcg ccgtaaattt
     6181 tgaaaccgcc tggctttctc gggacatttg gtttttacca aagaaccctc atgccctaca
     6241 gaaatatgat catcaaaaag gagcacacat gtcgaagact cttccttcct gggaccttca
     6301 aggtctcttg cgccatccga ccaaagactt caagcggatc accaaaaagc ttgactccct
     6361 catttccgaa ctcgaggtag cacgaccgca gttatccccg gatatttctg ccgcccgttt
     6421 caagaaaatc tgggagcaat acgaaaccgt caccgagtac atgacgacgt tgcgggcctt
     6481 ttcgttttta tggttttcag aaaatacgaa aaatcaagaa gcccgggcat ttgacaccca
     6541 ggtacgaaac agactgacag actgttctaa tcgactggtc tttcttgacc tctggtggca
     6601 aagcctcgat cccaccaacg cggcccggct gaccgcaaaa gccgagcggt ttcggtacca
     6661 tctggagacc ctgactcgtt tcacacccca taccttgagt gagcctgaag aacggatttt
     6721 aaccattaaa agtacgaccg gtcgccaggc tctcgaaa
//
LOCUS       DATIAL010000072         7703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_12691, whole genome shotgun sequence.
ACCESSION   DATIAL010000072 DATIAL010000000
VERSION     DATIAL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12691"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VLA60_02345"
     CDS             <1..277
                     /locus_tag="VLA60_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSF08221.1"
                     /translation="RAAKMRKGPYMAWTVYMLECADSSLYTGITLDLERRLAAHAKGK
                     GAKYTKHRGPFTVVFTERQETRGQALKREAAIKSMKRKAKLGLIGTG"
     gene            complement(710..919)
                     /locus_tag="VLA60_02350"
     CDS             complement(710..919)
                     /locus_tag="VLA60_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08222.1"
                     /translation="MKTKTFDCVEMKHQGAEQLKKTIDQLTDQEKLAFWSERTRELKQ
                     RMAIAKTGKESSGNTSSTESSTMHK"
     gene            complement(2254..2466)
                     /locus_tag="VLA60_02355"
     CDS             complement(2254..2466)
                     /locus_tag="VLA60_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08223.1"
                     /translation="MWVLVRGIQEKIDGTCLVEAGFRPGSRATFVSAKVAKTIDAPFG
                     LIEEEGRQPCEERTNSLRSNKVRQLV"
     gene            complement(2568..2882)
                     /locus_tag="VLA60_02360"
     CDS             complement(2568..2882)
                     /locus_tag="VLA60_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08224.1"
                     /translation="MRFYILRNQVVVPASSAKEFGEWVETADRVVSHTQVADIEVSTV
                     FLGIDHQFFAGPPLLFETMVFGGDLDQTCRRCSTWDEAEAQHEEILSEVLNRVYGYYK
                     PG"
     gene            3526..3993
                     /gene="secB"
                     /locus_tag="VLA60_02365"
     CDS             3526..3993
                     /gene="secB"
                     /locus_tag="VLA60_02365"
                     /inference="COORDINATES: protein motif:HMM:NF004394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HSF08225.1"
                     /translation="MTEEQKPVFTIEKIYVRDLSLEVPHAPGIFREGGAPQVHVELKT
                     QHTRVEEGMYDASVTVTVTAKVNEKIMFIVEVEQAGLFRIRHVPESEMGPVLGIGCAN
                     ILFPYVRETVSDAVTRGGFPTVMLNPVNFEALYQQQQQQQQKQTTPDSAATIH"
     gene            complement(4446..4745)
                     /locus_tag="VLA60_02370"
     CDS             complement(4446..4745)
                     /locus_tag="VLA60_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSF08226.1"
                     /translation="MNIRTLDQHIEVTPEIAGGKARIAGHRITVQNIVIWHESFGKSA
                     DEIASEYGLTLADVYAALANYFDHREEIDQDITEHKAFIELLRQKTPSKLKEKVQ"
     gene            complement(5578..5856)
                     /locus_tag="VLA60_02375"
     CDS             complement(5578..5856)
                     /locus_tag="VLA60_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HSF08227.1"
                     /translation="MTLEAFQRDELVFDAVVRNLEIIGEAAKHWPRDIQEMMPEVEWS
                     KAAAFRDVIAHGYFGLNVHIIWDVARNKIPDISRSVEKLLTTLRNDQT"
     gene            complement(5921..6214)
                     /locus_tag="VLA60_02380"
     CDS             complement(5921..6214)
                     /locus_tag="VLA60_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSF08228.1"
                     /translation="MNHKNLVSLLSSRREEIVRRFAIKTLGIFGSAARDEMHEGSDID
                     VLVEFQSSPTFDAYMDLKFYLEDIFQTNVDLVLEDTVKPRMRPLIEKDLIRVA"
     gene            complement(6733..6957)
                     /locus_tag="VLA60_02385"
     CDS             complement(6733..6957)
                     /locus_tag="VLA60_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08229.1"
                     /translation="MLAVAWPFGCPARFTDTGGGQTRLAQTLPAFFPVPVARLGHATR
                     PGDHKGEERPSFGPAGRRRTTEETPFALQS"
     gene            7145..7348
                     /locus_tag="VLA60_02390"
     CDS             7145..7348
                     /locus_tag="VLA60_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSF08230.1"
                     /translation="MEYPIVIEQDPEAGGYVVYCPTLKGCVSQGETEEEALENIKDAI
                     KTYLESIEDLKRRKKMRTVEITV"
     gene            7345..7557
                     /locus_tag="VLA60_02395"
     CDS             7345..7557
                     /locus_tag="VLA60_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HSF08231.1"
                     /translation="MTKLPQVSHERVVRALKRAGFYLLREGKHISMTDDKHIVIIPRQ
                     HVIKPGTLKQILDGAEISSEQFRELL"
BASE COUNT         1862 a         2062 c         1973 g         1806 t
ORIGIN      
        1 ccgggctgca aaaatgagga aagggcctta tatggcgtgg acggtctata tgttggaatg
       61 cgccgattcc agtttgtaca ccgggatcac ccttgatctg gagcgtcgtc ttgccgcgca
      121 cgcgaaaggg aagggggcca aatatacgaa acatcgtggt ccctttaccg tggtgttcac
      181 cgaacggcag gagacgagag gccaggcact aaagagagaa gcggcgatta aatccatgaa
      241 gcgaaaagca aagctgggct taatcggcac gggctaagac gctcgcggcc ccgtcatgcc
      301 tctgttcctc atccgtcatc accatctttt tcctgtctgt catgccagac gcgagtcatc
      361 ggataccaat cttattttcc gcatggtcat cctgatccat tcgacaaggc tcaggacaag
      421 ggcttcggcg aaggatctgt gccccaagct acaccccgca gggctagcga agagccatcc
      481 tcccttcgtc atgcccgaca tttcccatcg ggcatccaac ttcattctct taacccatcg
      541 agaccaaaaa aaaccgaaaa gcagaattcc cacccgatgt aacgcgaggg caatgatctt
      601 tactaaagga gtatcggcat aggatatccg gaggggaaag gggggttctt gcagaaaaag
      661 cacagtaaga ggcaactggg agcgtactta tcgtctggat attcctcgat cacttgtgca
      721 tggtgctgga ctcagtagag gaggtgttcc ctgaagattc tttcccggtt ttggcgattg
      781 ccattcgctg cttcagctca cgtgtgcgtt cggaccaaaa tgccaacttc tcctgatctg
      841 tcaattgatc gattgttttc ttcaattgct ctgcaccctg gtgcttcatt tcaacacagt
      901 caaaagtttt cgtcttcata cacgatcacc tccagaggag agaatattga gaatgtgact
      961 tcatcctttc gtcctcaaca tcttctgtct tatgtcggct catagtcttc cttccggcgg
     1021 aactttaaca aatacgattc agcttagcat tataccgatc attttttggc ggaatggtcg
     1081 tatgggtctg gtgggtaaaa tcttcccatg agaacatcca tcttcaacag atgtggacaa
     1141 gaaccgtcag gcgaaggctt ttccgagaac tcgtcgcgtt ccgacctgcg gggtaacgta
     1201 tgctggtaat catcctgggc cagaggcgaa agaggtgtgt ccagagcgaa cctacataag
     1261 aacagcagag aggtttccct ggcatcattt ccgaccttgt tcaacgggca tccatcgtat
     1321 tccccgcatg gtcatcctga tccattcgac aaggctcagg acaggggctt cggcgaagga
     1381 tctgtgccca gcccaattct gtcaatcgaa acacgacgct cctctgtcgc ttcccacgtt
     1441 gatcatccgg catcttttcc gggaagcccc caagaaggtt ccgaccccaa ccctgagagg
     1501 agaaaaggaa ggtaggtgta tctaaactat caccttcagg tcggccaacc gggctgacgc
     1561 tcccataaga ggcggaccgt gtttgagccc tgcgagttgg accgcccctt cgttggcctg
     1621 cgccggcacg tatttgtttt gacccgacgc ctgccggctg gcccacagga gggacgctct
     1681 tatcagccag cggaccgtgt ttgagcctgg cgagttggtc cgctcttcgc agggttggcg
     1741 tccctcctct gcagtcaggc cagacggggc gtcaatggtt ttgggtcctt ttgccgaaac
     1801 aaaaggacct cggctgccgg gccgaaaccc ggcatcaaaa agacaaattc catctaaaca
     1861 atcgggaagg aaatgggctg tgagtctgtg tgagaagaag gttccgatcc caagtactgg
     1921 taggcgaaat tgaaagaagg tttaggttac ctccaccttc aggtcggcca accgggctta
     1981 ccttaaaatc gacaggcgaa ttgttgatcc gacgcctgcc ggctggctcc agggagggat
     2041 gctcttatcc gttggcgggc cttgtttgag cttggcgaat tggtcctact tcttttctga
     2101 gtgcaaccgg caccggagaa aaggcgggca gtgtttgagc ccttcggctt tgctcaggac
     2161 aggctccgcg agttggtcgt cctccatgga cctgcctcgg cacgcatttg ttttgacccg
     2221 gcgcctgccg gttggcccaa aggagggacg ctcttacacc agctggcgga ccttgtttga
     2281 gcggagcgag ttggtccgct cttcgcaggg ttggcgtccc tcctcttcaa tgaggccaaa
     2341 cggggcgtca atggttttgg ccacttttgc cgaaacaaaa gtggctcggc tgccgggccg
     2401 aaacccggcc tctacaagac aagtgccatc tatcttttct tggatgcctc tcacaagaac
     2461 ccacatagaa aattaaacca ttctgaagtg ctgtgccgct ggaaggggct tggagtatgc
     2521 gtgaatgaga aaccaaacag attcaagacc aaacgaacgt cccaggttca ccccggctta
     2581 taatagccat acacccggtt taacacttcc gacaaaatct cttcatgttg cgcctccgct
     2641 tcatcccagg tggaacaacg gcggcacgtt tgatccagat cgccaccaaa caccatcgtt
     2701 tcaaacagga gaggggggcc ggcaaagaat tgatgatcaa tcccaaggaa cacggtagac
     2761 acctcaatgt ctgcgacctg tgtgtggctc acaacccgat cagccgtttc cacccattcg
     2821 ccaaattcct tggcactcga agcgggaacg acgacctgat tacgtaaaat ataaaatcgc
     2881 atgctcagac ctcccggcta gattcgacgc caccgtggtc tcttcctctc tagcatagga
     2941 catgatgcca atctctggcc ttccacatca acctgggcca tggaatttcc tcatctgtaa
     3001 agtaccgggc tgacgtgcga atcaccaggc agcccgtgga cctgacgcct tccggctggc
     3061 acaaaggagg ggcgctctta tgagctggcg gaccgtgttt gagcccttcg aaaactcagg
     3121 gcaggctccg cgagttgggc cgctctctgc aaagttagcg tccctcctct tcaatgaggc
     3181 cagacggggc gtcaatggtt ttgtccactt ttgccgaaac aaaagtggct cgtctcaggg
     3241 gggcgaaacc ccggcctcta aaatccaagt gccacaagga cccatagaga aaagtgagtc
     3301 atgctcaaca ggcaggaatg ggtgaagggg cctgggagta tctagaaaat aaaacccttc
     3361 cccgaacccg cctcttcatg aagaagaaaa tggaagccgg gttgcgtcgc atgaggaatc
     3421 gatgacaata tgggtttctt ttttttcaga atgccggaag atcgggttgg tcaggcccgt
     3481 ttgtggcttg atgaccaact ctattcacgc acacgaggat gacccatgac tgaagaacag
     3541 aagcccgtgt ttaccataga gaaaatttac gtgagggatt tgtccttgga agttccccat
     3601 gcacccggca tttttcgtga gggtggagcc cctcaggttc atgtcgaatt gaagacgcaa
     3661 catacccgtg tggaagaagg tatgtatgat gcgtccgtga cggtgaccgt gacggccaag
     3721 gtcaacgaaa aaatcatgtt tatcgtggag gtggaacaag ccggcctatt tcggattcgg
     3781 catgtccccg aatcggaaat gggtccggtg ttagggatcg ggtgtgcgaa tattctcttt
     3841 ccctatgttc gtgagacggt gtctgatgcg gtgacacggg gagggttccc gaccgtcatg
     3901 ctcaatccgg tgaattttga agccctgtat caacagcaac aacaacagca acagaaacag
     3961 acgacacccg attccgcggc caccatccac tgaggcagct tcccgggcga agtgctatct
     4021 cgaagggagg gagcactaga taggaaaggg aggcggtaga ggcccgaaat ccgttttttg
     4081 attttgagca gagattgaaa ttgaaagggt atattcccta taaaaattca acctggtctt
     4141 gcatgtcttc tggatccagg acctgggaga tcaacagcaa tccttggatc atctcgccaa
     4201 tagaaattcc ttgcggtgcg taaacgattc cgctgtgttt tttgcctgcc gcatggaaac
     4261 gtagaaaatc atcatcttaa gtgacgatca cccggttttc actgtgagct cttttgaaat
     4321 gttccgcatc cgaagcactt agcatcccag tttcctggac agcgagtatg caacgccacg
     4381 ttggcgtaac cccaggatga tggctttggc aatatgctca tcagcataga atcgtatctt
     4441 ttgactcact gaaccttttc cttcagttta gaaggagtct tctggcgcaa taattcaata
     4501 aaggccttgt gttcagtgat gtcctggtca atttcttccc gatggtcgaa atagtttgcc
     4561 agtgcggcat agacatcggc taaggtcagg ccgtactcgc ttgcgatttc atctgcactt
     4621 ttgccgaagc tctcgtgcca gatcacaata ttctgcacag taatgcgatg cccggcaatg
     4681 cgtgctttgc cccctgctat ctcgggcgtc acttcgatat gttgatctaa ggttcttatg
     4741 ttcattggag cgctcccttt tttctaagct gaaaatcgta tgttagcaat gtaacaaaga
     4801 atttgtaagc cagaattggg gaaaccttta ctgcaagctt tcttgcccct ccgtaattcc
     4861 tccagtcgtt aaaggagtct ctccagttgt tatcctgagc ctccttccgc cttgtcatcc
     4921 tgagcagcag cgaaggatct gtgcccagcc tcatcccgcg tatcgaaaca ttacgccctt
     4981 ccgtccttcc aggtggtcgt cagtggggag gttgcgcgca aggtgggagt gatatgtgaa
     5041 tggaagccct gaccctgcag cccgaacgct gtttcttctc tagtccgacg cctgccggct
     5101 ggccaatgga gggacgttcc taatccgctg gcggaccgtg tgtgagcctg gcgagttggt
     5161 ccgccctccc gctccgtcat ccccgacatt cgttatcggg gatccatctt ccttgtattt
     5221 tctggggcat ccctggcctg gtggtatggc cgagccgtgc aaccggcccc ggagaaaagg
     5281 cgggcagtgt ttgagcgcag cgagtttgcc cgccaccggg gccggtgaac cgcgcagggc
     5341 acccgaaggg ccatgccacg gtcaaaatgg ttttggctac ttttgcccaa acaaaagtag
     5401 ctcggctacc gggccgaaac ccggcattta aaaagacaag tgccacaaag acccgcagag
     5461 aaatacaaca tttgactccg attgtgaaag acaggagagt tgaaggaaat ttagcggcag
     5521 gcgtggttga aagacctcac cacctcattt gttgactgag atgagaaatc agacggctta
     5581 agtttgatca tttcgtagtg ttgtcagcag tttttcgaca gacctggaga tgtctgggat
     5641 tttgttccgg gccacatccc agataatgtg gacattcaag ccaaagtagc catgggcgat
     5701 cacatcacga aatgcggcgg ctttggacca ttcaacctcc ggcatcattt cttggatgtc
     5761 tcttggccaa tgtttggcgg cctcgccgat aatttccaaa ttgcggacca cggcatcgaa
     5821 aaccagttca tcacgttgga aggcttccag tgtcaggccg ttggtctagc gaagcacttt
     5881 attggcggct tccagaatat cctcaaggac aaacgtccaa tcacgcgaca cgaatcaaat
     5941 ccttttcgat caaaggacgc atgcgaggct tcacggtgtc ctcaaggacc aaatcgacat
     6001 tcgtctgaaa aatatcctcc aaataaaact tgaggtccat ataggcatcg aaagtggggg
     6061 aggactgaaa ctccacaaga acatcgatat cgcttccctc atgcatttca tcacgggcgg
     6121 ccgacccaaa aatacccaat gtcttaatgg caaatcggcg aacaatttcc tcgcgcctgc
     6181 tgctcaataa cgaaacaaga ttcttgtggt tcatgacatc gcctccacat gaaagtatgc
     6241 tccctgccat ccaaaaagat gtcagagcca tccagacaac gaataggtgt cttctgtcag
     6301 ttcatcgtca tctttctggc tgaaccttac caaaaacgat tcaggttagc attatcccaa
     6361 ttttttggca gatggtctgc atgtgtcgta tagtcccgct tgatgcgaag actcatcctt
     6421 gcatccaata gaggcatcct taaccgtggt attttccctt tgcaaaggcg gtgggccata
     6481 accatgtggc ctaccggaca actgttatgg gacattaatc ttcatgcagc catgaaacat
     6541 gaggaggaaa gatccccggc caaaaggcga gacttcccct ttcgttatcg ggaaagccca
     6601 ggaggtttct ttcggatggt tcccggacga gagcagcctg gcaaaggaaa aatgggttgt
     6661 catcctgagc ccttcgactt cgctcaggac aagggctcaa taaggatctg tgcccagcta
     6721 tgaatggaag ccctaactct gaagcgcgaa tggtgtttct tctgtcgtcc gacgcctgcc
     6781 ggctggccca aaagagggcc gctcttcccc tttatgatcc cctggcctgg tggcatggcc
     6841 gagccgtgca accggcaccg gaaaaaaggc gggcagtgtt tgagccaggc gagtttgccc
     6901 gccgcccgtg tcggtgaacc gcgcagggca tccgaagggc catgccacgg ccaacatggt
     6961 tttggccact tttgccgaaa caaaagtggc tcagctgccg ggccgaaacc cggcatctct
     7021 taactcacat cactctcaac gttatgcccc agctaaaaga tcaaaatctg acctgcaatt
     7081 ccctggaagg gaacttctgg tatactttag ccacatcaac agaggtatca atggggaggt
     7141 cggcatggaa tatccaatcg ttattgaaca agaccctgaa gccggagggt atgtcgtcta
     7201 ttgtcccacg ttaaaagggt gtgtttcaca aggggagact gaggaagaag ccctggaaaa
     7261 tatcaaggat gccattaaga cctatctcga aagtattgaa gatttaaaac gacgaaagaa
     7321 aatgcggacc gtcgaaatca cggtatgacc aaactcccgc aggtctctca tgaacgcgtc
     7381 gtgagagccc tcaagcgtgc aggtttctac ctccttcgag aaggcaagca catttcgatg
     7441 actgacgata aacatatcgt catcattcct cgccagcacg tcatcaaacc gggcacatta
     7501 aaacagatcc tggacggggc ggaaatatcc tccgaacagt tccgggaact tctctaggtt
     7561 gccgcctcat cgcaattctc acgggcaacc agcactcatc cgccatcaag cacccttcat
     7621 cacatctccc catcaccttc cggtcggcca aacgggctgc ccccccaaat caccagacgg
     7681 accgtagtcc cgacgcctgc cgg
//
LOCUS       DATIAL010000073         2359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_120418, whole genome shotgun sequence.
ACCESSION   DATIAL010000073 DATIAL010000000
VERSION     DATIAL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120418"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            748..1338
                     /locus_tag="VLA60_02400"
     CDS             748..1338
                     /locus_tag="VLA60_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08232.1"
                     /translation="MGLIVFSGLTDHAQAKVIEPEESDSVVMSGAGGIFYPFQGQSGV
                     SGTIQAMGIISPQERMGVELEYRKFDTELFHAKNIDMQSYILRGIGQYFFRTHGISPY
                     VGLGVNIALNVFDENDVEKKRTSINVKGDKGFGYGIMGLLGVEAPVGQGVAVFAEGRV
                     SADFQLTRYTNASGKNKIDVENLSGLTGMVGIRFHF"
     gene            1966..>2359
                     /locus_tag="VLA60_02405"
     CDS             1966..>2359
                     /locus_tag="VLA60_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08233.1"
                     /translation="MRDNAVTWLLLGALLGGVINAAVRRYATFKEGKGIALAIQGEIT
                     AIIEIVLKRRYLQHLEKILQKLSDPAHQLTDNDFFSPRIRENYFPVFNSLANKVGLLV
                     GRSDTVADFYTKAKSIIEDIHALEEMRDK"
BASE COUNT          647 a          555 c          538 g          619 t
ORIGIN      
        1 gaaccatggg ttctccgaca tctttcgcac tgatgcgaaa cgattcagga tgtgcttgta
       61 gcccgcaaag catgagagtt tgataggcaa tcacatccgt cgatattaac tcgcgaatta
      121 tcatgcttgt cccgcagacc tccgtgtatt tctttggcac tcaaaaataa aaggatggat
      181 ccgccttagg agcagatccc ggatttcccg actgtattat agaccatcca ttcctccgga
      241 taatacgcga tcatcggtct gatttcagtt ccgtccctgc cctacggaca ttgattacaa
      301 catcatctgt caacaaacgc catcatgacc ctcccagttc acctttacca tcaagtggtt
      361 tgcaaccaac gtggggacag tcttggccaa gtgacaaaat cagtcacatg gttggtccat
      421 gataataaat aaaattaagt cgaaaaaggc aaagcgaagc attcaacaaa cattatcccc
      481 tatataaaat ttaagtttgt acaacttcct cgccgataga gagcttagcg agtttgtccc
      541 cgttctatca gggaggtcaa tgatcatctc aattatttcc cttcctcctg taatcgcatg
      601 acaagtcctt ctccccattg ttaccaaaaa tttcttgtgg tctgaatcag agctatggat
      661 gttcctttac cactatcaac aagccaatgc acaacccctc gagagcccct gaaccgatta
      721 gttttaatgt attggctagc cctcctaatg ggactcatcg tgtttagtgg actaacggat
      781 cacgctcagg caaaagtcat agaacctgag gaatcagatt cggtggtgat gtcgggtgcc
      841 ggagggatct tttatccgtt tcagggtcaa tccggggtga gcggtaccat tcaagccatg
      901 ggaatcatct ctcctcaaga acgaatgggc gtggaattgg aatatcgaaa attcgacacg
      961 gagctgtttc atgcgaaaaa tatcgatatg caatcctata ttctcagggg aatagggcag
     1021 tatttttttc gtactcacgg catttcaccc tatgttggcc taggagtgaa tattgccctc
     1081 aatgtctttg atgaaaatga tgttgaaaaa aagaggactt cgatcaacgt aaaaggagac
     1141 aagggatttg ggtatggcat tatgggatta ttgggtgtcg aggcccccgt gggacagggt
     1201 gtcgccgtat tcgcggaagg ccgggtgagt gcagattttc agttgacgcg gtatacgaat
     1261 gcctccggga aaaataaaat tgacgttgag aatttgagtg gcctaaccgg aatggttgga
     1321 attcgatttc atttttgacc tgccattgaa gcgatatctt cacagtacat cctaattggg
     1381 cggcatctga ctcactcaga tccccactac ctgcactccc gccgcttgct cttattcata
     1441 tacaccgcct gagcacttca gatcgcaggt ttggggatac gccttctctg cgctgaccgg
     1501 agcgagaaat ttcttacggc tgcttcccga gtagaatatc gatgtcgcgt agggggaatt
     1561 caaggcaaac ctggcctgag gatctcctga tttgcctcga caaccttttg aacttaatca
     1621 acgggaaaac cagtttgaga aaccgaattt cttgatcttg ttcatgaaaa attcactcaa
     1681 cccattttac ccttcacgaa caaggtgtaa ttgggtttct ctgcgatagg atcgttatcc
     1741 tgaaccaagg acatatgatc tgtgccccgg tggaaaggcc aaaaacgggt tcttcctcaa
     1801 atgatagaat aagatcagtt gtggacctgg aaagatggga aaggaagtga ggcatctgac
     1861 catgcccggc atggtcttgc aaaaatcacc gtaaagttct aatttaagga ggcccgggat
     1921 gaaaaccctc cgggagaact cttctccgtt tgaaaaaagg actacatgag agacaatgct
     1981 gttacctggc ttctcctcgg agcgcttctt ggaggcgtca tcaatgcagc cgtcaggcga
     2041 tacgccacct ttaaagaagg caaaggcatc gcccttgcca tacagggaga gatcaccgcc
     2101 atcatcgaaa ttgtcttaaa acgacgatac ctgcaacacc tcgaaaaaat ccttcagaaa
     2161 ctttccgacc cggctcatca gctgacggac aacgattttt tttcaccaag aattcgcgaa
     2221 aattatttcc cggtgtttaa cagcttggcg aataaagtcg gcttgcttgt tggccggagt
     2281 gataccgttg cagattttta caccaaagca aaatccatta ttgaggatat ccatgccctt
     2341 gaggagatgc gggacaaat
//
LOCUS       DATIAL010000074         7361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_765830, whole genome shotgun sequence.
ACCESSION   DATIAL010000074 DATIAL010000000
VERSION     DATIAL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7361
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765830"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1372)
                     /locus_tag="VLA60_02410"
     CDS             complement(32..1372)
                     /locus_tag="VLA60_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HSF08234.1"
                     /translation="MGITSAFFAATSGINATSRALSEIGNNIANAQTIGYKSRSVSFG
                     DLFGATIGGGGTSTALVEGRGVRVLGIDPSFTQGSLQTTSNALDLAIDGDGFFRVSDA
                     TGNIFYSRAGQFDIDSQGNLVNPAGLRLQGDQADDTGLLTGSIGNLTLTTSTQPGVQT
                     SEITMSGRLAANAPINAYTTAQVLDPQSNSGLFPGSTSVRVFDSQGRGHDLTVYFAKT
                     ANNTWDYNVIAPDSEVTVPVGDSNPTTGNALVAQGTLTYTPDGFLSLESDPSGHDITF
                     TGGVDAPQTIIFDFGTSITEGGAGTDGLLQQGESLNNKGLILDTLSQDGYGPGSLAGT
                     SIGEDGVITGRFDNGTTRTLGQVRLTRFINPDGLQPIGRNLFIQSGDSGTPLDGVPGT
                     GAFGKVSASTLEASNVDLGEELVNMITMQRGFQANSRIITTTNDLLGELVNLAR"
     gene            complement(1436..2104)
                     /locus_tag="VLA60_02415"
     CDS             complement(1436..2104)
                     /locus_tag="VLA60_02415"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HSF08235.1"
                     /translation="MEVTPTQPGTNATATGGANVQAAVSALGSDVFLRLLVTQLQSQD
                     PTNPVQNEDFVAQLAQFTTLEQATSTNKLLEKLIGQDTQRTQLDLVNLIGRTVVAQGD
                     TVSIAGEEQPTLGYALSGEARSVTIKVLDSNKQVVRTLESTDVQKAGANQVQWDGLND
                     SGDRVPDGVYRFIVKAEDANKQPVPNFTFARERVMTIAFGAENPVVLQSGKSLHTEDI
                     LSIL"
     gene            complement(2163..3743)
                     /locus_tag="VLA60_02420"
     CDS             complement(2163..3743)
                     /locus_tag="VLA60_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08236.1"
                     /translation="MINALPILFGLLESPPHVTRQAGGVGLDQAGTTENPSKNLFAKI
                     FRNQTTAASFLNSVSKTRSGEDGGRGSKLDWLHGTQALSGHTLPSLYLASLLENNGQE
                     VPEEGAVVGNSSIPLRAEGVNGNSAGGQVENQTALFGLLPGNGSQDESVVNGNVAPLA
                     FLQGKPGGEIRQPVGLGIQAGLPLLAGAPSSQTVVIGSTLADQSAAPFVPKIDSERVL
                     QNALAAVAGKAVTESDANQVNTVDRVLSPVQDTGPRLLQNPPSPIGPPQASVKLANTP
                     VNQAFQVPKPFDETPAGIADAAQLSQNRLKYVSGVSSVLSESPANGKESSIGIPLDRL
                     ADANVSLTGDRSRDVMEVTGKAEGVDPDAGQGLNHGMGNPTHSQSGFQQSSSVSSPGQ
                     AVRMAEERVPDLPGPALQRLQMEVQLSETNRVQIDVGVQHRQVYASLLMDQATLKNLA
                     VQFVPQLEEQLTQGEMELQEFSAEVRDHQSGQESDTRSDGAGRQTSQRGTTSLHQTPG
                     SLSYKAGRAEEQGFYLVA"
     gene            complement(3747..4589)
                     /locus_tag="VLA60_02425"
     CDS             complement(3747..4589)
                     /locus_tag="VLA60_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08237.1"
                     /translation="MSKKMSVQPMHDLDHQPNNGAFNMARDGSTLGTERNEWRSGERR
                     DLLASRYTPHTSRFPLRASQTQGLSWRQFSTLLSRTMLPSRSLIAWWVLAMILWATTV
                     GSVQETPEVGTTDSFENQAVENGLNEEAPSAETTLLEQLQARLKEVEERERGLQQREE
                     RLMALQQDLEALAARQTKEGKRLVGEASSLAEEQRRYVEQDPALIHLIKIYESMDPEE
                     AALRIAEMREGLALDILAAIKDKKAAGVLAGVEPVKAARLSEGLRRYRDIKLNQRQNS
                     KMQN"
     gene            complement(4601..5047)
                     /locus_tag="VLA60_02430"
     CDS             complement(4601..5047)
                     /locus_tag="VLA60_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HSF08238.1"
                     /translation="MNWTTLLRFRKQVEDLAREEVVLAEWEKSQIVSKRDDLMVEMEM
                     VATELDQRIREGIDNMIAEQRYQWLDQISTAIEQQSQQIQIAEAKLVDLRATLKKAHH
                     ARRVVELVIAKKEEEVMQKIATQEQQMQEDVTAHAYATSQLEEMIQ"
     gene            complement(5044..6387)
                     /locus_tag="VLA60_02435"
     CDS             complement(5044..6387)
                     /locus_tag="VLA60_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HSF08239.1"
                     /translation="MSLTTIQQRLDDIAPVTITGRVVKAVGLTLEGTGLGASVGQRCH
                     ILSKRGQSMVEGEVIGFREQRVVVMPFGAVRGIAAGNLIRYDPTSPRLLVGPQMLGRV
                     VDGLGQPLDEKGPLSRSRRYALYAPAPGPMLRERITTPMDLGVRAINALLTCGVGQKL
                     GIFAGSGVGKSVLMGTMCRHTSADVNVIALVGERGREVREFLERDLGREGLRRSVVVV
                     ATSDQSPLVRVRAAFVATAIAEFFRDQGNQVLLLVDSLTRLAHAQREVGLAAGEPPTT
                     KGYPPSVFTLFPQVLERVGPLGTGSITGLYTVLVDGDDLNDPIADSIRSILDGHIVLT
                     RRLAMQGHFPAIDVLQSVSRVMSDIASTAHQDGARFLVQMMAEYRNAEDLINLGAYQL
                     GTNPRLDAAIHMKGPIDAFLRQSREEGVGILESCQGLETLAQQGRRLLERKGKTV"
     gene            complement(6384..7022)
                     /locus_tag="VLA60_02440"
     CDS             complement(6384..7022)
                     /locus_tag="VLA60_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HSF08240.1"
                     /translation="MRSKWSKVRPLMEQDDTVKRFQMVELVHKSAKQRAEEAQPHDCR
                     ELQQAAFERGRQAGIAEGRAQCQAQVEEEVKKALRLANQIGRARVIALEEHERDIVEV
                     ALAISKKILLRECEIDKELVVRQVRQVLGLLPDQTLVTLKVHPQDFKILEPLQHTLRP
                     EWADGDHLALEAFDDVDPGGCLVEQAGLLFDARLTQQLALVASEFGLEAPRS"
     gene            complement(7003..>7361)
                     /locus_tag="VLA60_02445"
     CDS             complement(7003..>7361)
                     /locus_tag="VLA60_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HSF08241.1"
                     /translation="LTSLESKNPEIAEEIRGKMFVFEDLENVDDRGMQELLREVSKEE
                     LLLALKPIDGPLRDKFFKNMSSRAAESLMEDMETRGPVKLSDVETAQQNIIKTVQRLA
                     GEGRVALGGKGEEQMV"
BASE COUNT         1580 a         2185 c         1755 g         1841 t
ORIGIN      
        1 gggcttgatc agttagatcc tcagtgaaaa tctaacgggc cagattcacc agttctccga
       61 gtagatcgtt agtggtggtg atgattcgtg aattcgcctg gaatccacgc tgcatcgtaa
      121 tcatattgac taattcttca cccaaatcca cattggaggc ttcaagagtg cttgccgata
      181 ccttgccaaa tgcccccgtg ccgggcaccc cgtccagggg ggttccggaa tcaccggatt
      241 gaataaataa atttcgtcca atcggttgca agccatcagg attaataaac cgggtaagtc
      301 tcacctgtcc aagggtccgg gtggttccat tatcgaagcg gccagtgatc accccatcct
      361 caccaatgga agttcccgca agtgatcctg ggccataccc gtcttgggat aaagtgtcga
      421 gaatcaatcc cttgttattc aaactctcac cctgttgtaa taggccatct gttccagctc
      481 ccccttcggt aatacttgtc ccaaaatcaa aaatgattgt ctgtggcgca tcaactccac
      541 ccgtaaatgt aatatcatgc cccgaaggat cactttctaa tgacaaaaat ccgtccgggg
      601 tataagtcag agttccttgg gccactaagg cattgcctgt agtgggattg ctatctccga
      661 ctgggacggt cacctccgag tctggtgcaa tcacattata gtcccaggta ttattagctg
      721 ttttagcaaa atacaccgtt aagtcatgcc ccctcccctg agaatcaaaa acccggacag
      781 aggtacttcc tggaaacaac ccactatttg attgaggatc gagaacctgt gccgtggtgt
      841 atgcgttgat tggagcattg gccgccagac gtccagacat cgtgatctcc gatgtttgga
      901 cccctggttg agtgctggtg gttaaggtta agttgccaat actcccggtt agaagtcccg
      961 tatcatccgc ttgatcacct tgcaatcgca gacctgccgg gttcaccagg tttccttgtg
     1021 aatcgatatc aaactggccg gcacgggaat agaaaatatt tccggtggca tcactcaccc
     1081 ggaaaaatcc gtcaccatca atggccagat ccaaggcatt ggacgtcgtt tgaagcgatc
     1141 cttgcgtaaa gcttggatcg atccccaaca cccgcacgcc acgaccttcc actaaagccg
     1201 tgctcgtccc tccaccaccg atcgtagctc caaagagatc gccaaatgag accgaccggc
     1261 tcttatagcc gatcgtctgc gcattggcga tattgtttcc aatctcactg agcgctcggc
     1321 tggtggcgtt aattccactt gtcgcggcaa aaaatgctga ggtaattccc attggcgtgt
     1381 actccttgtt tctcgattga ccaatctaat gctgtttccc ctaccaccgt ccacttcaaa
     1441 gaattgaaag aatgtcctcc gtgtgtaagc tttttccact ttgcaggacg accggattct
     1501 ccgccccaaa ggcaatcgtc atgacgcgtt cacgagcaaa tgtgaaattc gggacaggct
     1561 gtttattcgc gtcttcggct ttcacaatga accggtagac gccgtccggc acccggtccc
     1621 ccgaatcatt caacccatcc cattgaacct ggttggcccc ggccttttga acatctgtgg
     1681 actctaatgt tcgaaccacc tgcttgttcg agtccagcac cttgatggtg accgacctgg
     1741 cctctccgct taaggcatag cccagcgtgg gctgttcctc cccggcaata ctgacggtat
     1801 caccctgggc cacgaccgtc cgtccaatca aattcaccag atctaattgt gtccgttggg
     1861 tatcctggcc gatcaatttt tccaagagtt tattggtact cgtagcctgc tccaatgtgg
     1921 tgaactgcgc taactgcgcc acaaaatcct cattttggac cgggttggtg ggatcctggc
     1981 tttgcaactg cgtgacgagc aagcgaagaa agacatccga accaagtgcc gaaaccgccg
     2041 cttgcacatt ggcaccgcct gtcgccgtgg cattcgttcc tggttgtgtc ggcgtaactt
     2101 ccataatgtc gggctcctat tttttccagc cttctttata cgagggcatc attgcatctt
     2161 ctttacgcca ccaagtagaa tccctgttcc tcagcccgcc ccgccttata ggagagcgat
     2221 cctggtgtct gatggaggga cgtcgtaccc cgttggctcg tctgccttcc cgccccatcc
     2281 gaacgtgtgt ctgactcctg accactctgg tgatcacgga cttccgcgga aaattcctgc
     2341 aactccatct cgccctgggt cagttgctct tccaattgtg gaacaaattg gaccgccaga
     2401 tttttcaaag tggcttggtc catcaacaag ctggcataaa cctgccggtg ctgcacgccc
     2461 acatcaatct gcactcgatt ggtttcggaa agttgcacct ccatttggag gcgttgaagc
     2521 gcggggccgg gaagatccgg gacacgctct tccgccatcc gaacagcctg tccaggggaa
     2581 gaaacagatg aggactgctg aaagcccgac tgagaatgtg tcggattacc catgccatga
     2641 ttgagcccct gcccggcatc gggatcgact ccctcggctt ttcctgttac ctccatgacg
     2701 tcccttgaac gatcgcctgt taaagacaca ttggcatcag caagacggtc caggggaatc
     2761 ccgatcgagg actccttccc gttcgccggc gattcactca gaacggacga aaccccgctc
     2821 acatatttca atcggttctg actcaattga gccgcatccg caatacctgc gggcgtttcg
     2881 tcaaaaggct ttggaacctg aaacgcctga ttgactggag tgttcgcaag cttgactgac
     2941 gcctgcggag gccctatcgg tgatggagga ttttgcagca aacgaggccc ggtatcttga
     3001 acagggctca agacgcggtc caccgtattc acctgattcg cgtcagattc tgtgacagcc
     3061 ttcccagcaa ccgctgcgag ggcattttga agaacccgtt ccgaatcgat cttgggaacg
     3121 aaaggagccg cactctgatc ggcaagcgtg ctccctatca ctacagtctg acttgatggg
     3181 gcgcccgcca agaggggcaa gcctgcctga atgcccaatc ccaccggttg ccttatctct
     3241 cctccgggtt ttccttggag gaatgccagg ggcgccacat tcccattgac caccgactca
     3301 tcctgggatc cattcccggg caagagtccg aacagcgccg tttggttctc cacctggccc
     3361 cctgcgctgt ttccattcac accttctgct ctcaagggaa tcgacgaatt tcccactacg
     3421 gcaccttctt cgggtacttc ttgcccattg ttctccaaaa gactcgccag gtaaagggac
     3481 gggagggtgt gaccagaaag ggcctgggtg ccatgaagcc agtccagttt cgaccctcgt
     3541 cctccgtcct cccctgacct cgtcttggat acactgttca gaaatgaggc cgctgtcgtc
     3601 tggttacgaa aaattttagc aaacaaattt ttggaaggat tctccgtggt gcccgcttga
     3661 tccagcccga ctccaccagc ctgacgggtc acgtgtgggg gactttccag aagtccgaaa
     3721 aggattggca aggcattgat catcgtttaa ttctgcatct tggagttctg gcgttggttc
     3781 agtttaatgt ctcgatatcg cctgaggcct tctgacaatc tggccgcttt gaccggttcg
     3841 acgcctgcta acacgcccgc ggcttttttg tctttgatcg cggccagaat atccaaggct
     3901 aagccttccc gcatttctgc gattcgcaac gccgcttctt cgggatccat ggattcatag
     3961 attttaatta aatgaattaa cgccggatcc tgttccacat accggcgttg ttcttctgca
     4021 agcgaagagg cctctcccac taaccgttta ccctctttgg tttgtcgtgc agccagcgcc
     4081 tctaaatcct gctggagtgc catgagccgt tcttcccgtt gttgcaaacc acgttccctc
     4141 tcttccacct ctttgaggcg cgcttgcagt tgttccaata atgtggtttc tgcactgggg
     4201 gcttcctcat tcaacccgtt ttcaactgct tgattttcaa atgaatccgt tgttcccacc
     4261 tccggggttt cttgaacgga tccgaccgtg gtggcccaca agatcatggc caacacccac
     4321 cacgcaatga gtgatcgact aggcaacatt gttctactca ggagtgttga aaattgccgc
     4381 cagctgagtc cttgggtctg tgaagcgcga agcgggaaac gggaagtgtg aggcgtatag
     4441 cgtgaggcca aaagatccct cctttctcct gacctccact cattcctttc ggtccccagc
     4501 gtgctcccat cacgagccat gttgaaggct ccattgttgg gttgatggtc aagatcatgc
     4561 ataggctgaa cagacatttt ttttgacata tttccattcc ctattggatc atttcttcaa
     4621 gttgagaagt ggcgtaagca tgagccgtca catcttcctg catctgttgt tcctgtgtgg
     4681 cgattttttg catgacctct tcttccttct tggcgatcac caactccacc acacgtctgg
     4741 catgatgggc cttttttaat gttgcccgta aatcaacgag cttggcttcc gcaatctgaa
     4801 tctgttggct ctgttgttca atggccgtac tgatctgatc caaccactga tagcgttgct
     4861 cagcaatcat attgtcgata ccctctcgta ttcgttggtc caattcagtg gccaccattt
     4921 ccatttcaac catcaggtca tcccgcttcg agacgatctg acttttttcc cattccgcca
     4981 agaccacttc ttcgcgggcg aggtcttcca cttgctttcg aaatcggagt aatgtcgtcc
     5041 aattcatacg gtctttccct ttctctcaag aagacgccgg ccctgttgtg ccaacgtttc
     5101 aagaccctgg cagctttcca gtattcccac cccttcctcg cgtgattgcc tgagaaaggc
     5161 atcgatcggt cctttcatgt gaatcgccgc gtcgagtcga ggattcgtgc ctaattgata
     5221 cgcccctaaa ttgatgagat cttccgcatt ccggtattcg gccatcatct gcaccagaaa
     5281 tctggcccca tcctgatgag cggtcgacgc gatatccgac atcacccgac tcacgctttg
     5341 taagacatcg atcgcgggaa aatgtccttg catcgccaac cgtcgtgtca acacaatgtg
     5401 tccgtctaaa atggaccgga tggaatcggc aatgggatcg tttaaatcgt ctccgtcaac
     5461 aagcaccgta tacagaccgg taatggatcc cgtgcccagc ggccccaccc gctctaagac
     5521 ctgaggaaag agcgtaaaga cagatggcgg ataccctttg gtcgttggcg gttccccggc
     5581 agccagcccc acctcccgtt gcgcatgtgc caaccgtgtt agggaatcaa ccaggagcag
     5641 gacctgattg ccctgatccc gaaaaaattc ggcaatggcg gtggccacaa acgccgcccg
     5701 aacccggact aacggcgatt gatcggaggt ggccaccacc accaccgaac ggcggagccc
     5761 ttcccgtccc aaatcccgtt ccaaaaattc cctgacttcc cgacctcgct cacccaccag
     5821 cgcgatcacg ttcacatccg cagacgtgtg ccggcacatc gttcccatca agacactttt
     5881 cccgactccg cttccggcaa aaatcccgag tttttggccg accccgcagg tcagcagggc
     5941 attgatggct cgtacgccca aatccattgg tgtagtaatg cgttcccgga gcatgggccc
     6001 cggagcgggg gcatagaggg catagcggcg gctacgggac aggggccctt tttcatccaa
     6061 cggctgcccg agtccatcca cgactcgccc caacatttga ggcccgacca ataatcgtgg
     6121 agaggtcgga tcgtagcgaa tgaggtttcc ggcggcgatg ccgcgtacag ctccgaacgg
     6181 catgaccacc acacgttgct cccgaaaccc gatgacttct ccctccacca tcgactgccc
     6241 tcgtttgctg aggatatggc atcgctgacc gaccgaagcc cccaagccgg tgccttccag
     6301 agtcagcccc acggctttga cgacgcgtcc ggtgatcgtc acaggagcaa tgtcgtccaa
     6361 acgttgttga atcgtggtga ggctcatgac cggggtgctt ccaatccgaa ttcgctggct
     6421 acaagtgcca gttgttgagt tagcctggca tcgaacagga ggccagcctg ctcaaccagg
     6481 catccacccg ggtcgacgtc atcaaaggct tcaagcgcca gatgatcgcc atcggcccac
     6541 tccggtcgta atgtatgttg aaggggctct aagattttga agtcttgcgg atggactttc
     6601 agcgtcacta acgtttggtc gggaagcagt ccaagaacct gacgaacttg tcgaaccacg
     6661 agttctttat cgatctcaca ttcccgtaac aggatttttt tgctaatcgc cagcgcgacc
     6721 tccacaatat cccgttcatg ttcttctaac gcgatcaccc gagcccgacc aatctgattg
     6781 gctaatcgaa gcgccttttt gacctcctcc tccacctgtg cttgacactg ggcacgcccc
     6841 tcggcgatcc cggcctggcg tccccgctca aacgcggctt gttggagttc cctgcaatca
     6901 tggggttggg cctcctcggc gcgctgcttc gcacttttgt ggactaattc caccatctga
     6961 aaacgtttga ctgtgtcatc ctgttccata agcgggcgca ccttagacca tttgctcctc
     7021 accctttcct cccaaagcta cgcggccttc accggccaga cgctgaacgg ttttaataat
     7081 gttctgctga gcggtttcca catcactcag cttgaccggg cctcgagtct ccatatcttc
     7141 catgagagat tccgccgctc gactggacat attcttaaag aatttatccc ggagtggacc
     7201 atcgatcggc ttcaaagcca gcagtaattc ttctttgctg acttcacgca acaattcctg
     7261 cattccgcga tcatcgacat tttccaggtc ttcgaacaca aacatcttcc cacggatttc
     7321 ttcggcgatc tccggatttt tgctttccag ggaggtcagg a
//
LOCUS       DATIAL010000075        10510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_980314, whole genome shotgun sequence.
ACCESSION   DATIAL010000075 DATIAL010000000
VERSION     DATIAL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980314"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VLA60_02450"
     CDS             complement(<1..317)
                     /locus_tag="VLA60_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HSF08242.1"
                     /translation="MNPLVQLREMGQSPWYDYIRRGLITSGDLQALIEKDGLMGMTSN
                     PSIFEKAIAGSTDYDEAIAQVASQVTSVKQIYEGVAIQDIQDAADLMRPVYETSDGRD
                     GYV"
     gene            complement(345..2435)
                     /gene="tkt"
                     /locus_tag="VLA60_02455"
     CDS             complement(345..2435)
                     /gene="tkt"
                     /locus_tag="VLA60_02455"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSF08243.1"
                     /translation="MKPACDSLDQLCINTIRTLSMDAVQAANSGHPGTPMALAPVAYW
                     LWNRILRSDPDDPIWPNRDRFVLSAGHASMLLYSLLHLCGVKSVNGQYERLGELSVTL
                     EDIKRFRQLESKCPGHPEYRWTSGIETTTGPLGQGVATSVGMAIAQRWMAGYFNRPDF
                     EMFNYNVYAICGDGCLMEGVSSEAASLAGHLKLSNLCWIYDNNKITIEGSTDLAFSED
                     VATRFIAYGWNVTRVGDANDLKMLDRAFGTFVKESERPTLIIVDSHIGYGAPNKQDTH
                     SAHGEPLGEEEIRLAKRYYGWPEDARFLVPDEVPAHFQQGIGKRGKELRSAWMARFAD
                     YQAKYPELAGHLYQMQHRQLPDGWDRKVPVFPPDAKGLAGREVSGIVLNALAAAIPWF
                     FGGAADLAPSTKTRLTFEGAGDLTGSNPSGRNIHFGVREHAMGAILNGLSLSKVRPYG
                     SGFLIFSDYARPAIRLSALMEIPVVHIFTHDSIGVGEDGPTHQPIEQISSLRAIPGLM
                     VFRPGDANEIAEAWKVIMELRHEPVIMIVSRQAIPTLDRMKYASASGVAKGAYVLADP
                     PDGCPEVLLLGTGSEVPLCVQAHEQLALEGIKSRVVSMPSWELFEQQSEDYRRSVIPP
                     DVTARVSVEQATVFGWGNYVGTQGHSIGMKSFGASAPLKELTKKFGFTVDHVVAAAKA
                     QIARHQAHAITADR"
     gene            2677..2871
                     /locus_tag="VLA60_02460"
     CDS             2677..2871
                     /locus_tag="VLA60_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSF08244.1"
                     /translation="MKVHLSHPTRDVVIQGPKRVADIFKELNLIPEAFLIIRGQDLMT
                     EDETVADGDSIEIRPVISGG"
     gene            2876..3811
                     /locus_tag="VLA60_02465"
     CDS             2876..3811
                     /locus_tag="VLA60_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08245.1"
                     /translation="MRCRKCPKRAVLGLPRHNTAFCGDCLTEFVRTQVQRAIKAQQMF
                     APEDRVLVAVSGGKDSLTLWEILLKLGYRVDALYVDLGIPGYSSRSKEKVEQFAKVVA
                     EPCGSQLTIHTVQEDAGAGIKELANLIKRPTCSACGTIKRYQFNRVAWQNHYDVMATG
                     HNLDDEAARLLGNVLQWQEEYLQKQSPNLPASVEGFAKKVKPLYRMTEREIAAYAVVN
                     RIDYLVEECPMAKGAKMLVYKDALNRLEAESPGTKQRFYWGFLDRQEKSSSVAPSMSQ
                     IDQTTLQPCTVCSQPTTAGTCSFCRMMARAKTAVK"
     gene            complement(4060..4491)
                     /locus_tag="VLA60_02470"
     CDS             complement(4060..4491)
                     /locus_tag="VLA60_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08246.1"
                     /translation="MDIELGKNTLRNTDGVFIAHGKEQLRIEWLEEEKKLALSMGVFM
                     PTGTEVAKLQRNVWEHNPGDRFVLTELPDSVKVEDTTLKTLVMEIHKKPHQAVAIPAA
                     KFYTSKGTLSEISPEWWRVGNKMELTGIDADLEGGSIELPE"
     gene            complement(4601..5590)
                     /locus_tag="VLA60_02475"
     CDS             complement(4601..5590)
                     /locus_tag="VLA60_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HSF08247.1"
                     /translation="MLFLVVGLAALVGIVLHIGLEPIQHTVSQLGLLHLGLILLPMIL
                     VYGLEALGWQLTLGSHTRHVGFMRLFAIRMAGETVNVTTPTAYVGGEPLKAYLLKRYG
                     VPMVDGLASVITAKTAMTFAQVLFILLGLAVAFWILGDSGHSWMAMLVSVGVLAFGVG
                     LFVLLQRHGLGMGCLGLLRACGIRFSFLEKREPQLKELDGTIRGFYSQHRQTFYAALA
                     VFFLAWMMETLEVYAILYFLGVRIDGWVALSIAALTVLIKGGTFFIPGSLGAQEGGYT
                     LLLMTFGYSEVTGITFALIRRLREILWIVFGLVCLMFLKGRGGEPDQMTSLER"
     gene            complement(5592..7076)
                     /locus_tag="VLA60_02480"
     CDS             complement(5592..7076)
                     /locus_tag="VLA60_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSF08248.1"
                     /translation="MDGILKQEATHAVDTAILLTSVGVFDPGAVGTGDAVPSPLTRVG
                     GMTLFQRAVFTLQRGGISQIWVLAGPEEQALRQLLREDSRVQAAVRWLPVREFPPHDP
                     QTWEALAEEINGACMIVGCHTVYSPALIQRLRAEGSQGKAVVVVGQPEEGYHRGNPGV
                     AFRPEGLEGRSTSTVVFHDQAVSQTSQSSTKRQTPDRSRLPLVGDLLVLPARLLGISG
                     VLHANGTNPLRLALEQAAVEGTIQTIGGASHWFRDVRGPKGPKLAERTLLEALQTMKG
                     GLDGLVDRYVNRKCSGLLTHGFLRLGWSPNMITMLSMVVGLVAAGLFVPGSWKLAILG
                     GLILQLSVIIDCCDGEVARLTFSESKFGQELDIWADNVVHIVLFAAIACGAFLQGPWE
                     HSHLPLLLGASAVLANVVSLLLVNHARQLRSRPREIRQLTEQEWANIEFMLGNVANRD
                     FSIMVLLSAGFGFLHWFLALAAIGSWLFVMSMAWMLRRSLIPRA"
     gene            complement(7087..7848)
                     /locus_tag="VLA60_02485"
     CDS             complement(7087..7848)
                     /locus_tag="VLA60_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HSF08249.1"
                     /translation="MKAIIFAAGVGKRLQGVTQGRPKCLVDLGGRTLLSRHVEALGQL
                     GVCQVVVVVGYAQDHIRKAMAADPFVQEVRWVVNEQFTRGSITSLWAARSEMDDDVVL
                     MDADVLYAPSILARLVHSPFPTALLMDETVKQESEECMIAAKAGRVLTLSKTLPSAYD
                     EAGEGVGFLKVQKQDIPALLQSVQAYVAAGALDMEYEDALKEFFEKVPVGYEKIGGLP
                     WIEIDFPEDIDRAESEILPAVINLEENTRATTLRP"
     gene            complement(7845..8933)
                     /locus_tag="VLA60_02490"
     CDS             complement(7845..8933)
                     /locus_tag="VLA60_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HSF08250.1"
                     /translation="MILFNPGPVNVSERVRQALLQPDICHRESEFSELLTDIRQKLLK
                     VFVPGEEDEYTAIVLTGSGTAAVEAALMSSIPTGKRAMVINNGVYGQRMSSMLAMQRL
                     GTPDLKYDWGIVPDPQTVDLALKQHPEVHTVAMVHHETTLGLINPVKEVAEVVDRYNR
                     VFMVDSVSGLGGEALDIAGNKLYMVAGAAQKCIQGFPGAAFVLVRKGFMERMMKYPKR
                     SWYLNLANYYEVQERGTIPFTPAVQVYYAFREAINELLEEGLDNRIQRFKGYATTIRT
                     RLEEWGVKPVLAPTLQSNTLTAFYLPEGCAYQGLHDELKRAGYVIYAGQGQLEEKVFR
                     IANIGALTNQNIEGFLSAFQSILSGARA"
     gene            complement(9002..9583)
                     /locus_tag="VLA60_02495"
     CDS             complement(9002..9583)
                     /locus_tag="VLA60_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF08251.1"
                     /translation="MIGPEEGVMQSRAQAMAAMLELMTDQLLIVCNGFPSREACKLRD
                     RTQNFYMIGSMGATAGIGLGLALAQPDKTVVVFDGDGNVLMSLGTLATISALRPKNLI
                     HVVFDNEVYGTTGNQPTYSRVVGLDKMAKAAGYKNVERVWEREDIVYEFKAMLGDDGP
                     SFLLVKVNEQLEDADRIALSPAELTKRFKGSVT"
     gene            complement(9736..10248)
                     /locus_tag="VLA60_02500"
     CDS             complement(9736..10248)
                     /locus_tag="VLA60_02500"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSF08252.1"
                     /translation="MSSNTVLEADDFVKGLQGIGFDFFTGVPDTILGGIIAHLTEERL
                     YTPAVREDEAVAMAAGAYLGGKIPVVLMQNSGLGNALNVLASLNLIYQIPCLLLVSWR
                     GFEGKDAPEHLVMGETMTTLLDTVRIPHRTLSQQTIVDDLKWTATTFMEKRIPVALLL
                     KKGIVKTIQP"
BASE COUNT         2709 a         3042 c         2410 g         2349 t
ORIGIN      
        1 ctcacatagc catcacgacc atccgaggtc tcatagaccg gccgcatgag atcggctgca
       61 tcctggatgt cttgaatggc tactccctca taaatctgtt tgacgctcgt cacctgcgac
      121 gcgacttgtg caatcgcttc atcatagtcc gtactccccg caatcgcttt ttcaaaaatc
      181 gagggattcg aggtcatgcc cataagacca tccttctcaa taagcgcctg aaggtcccca
      241 gaggtgatca aaccccgacg aatatagtcg taccagggac tttgccccat ctcacgaagt
      301 tggacgagtg gattcatggt gagattctcc ttgaaaggat gaagttagcg gtcagcggtg
      361 atggcatgag cttgatgccg tgcaatctgc gccttggccg ctgccaccac atggtcaacc
      421 gtaaaaccaa attttttagt taattccttg agcggagccg aggcgccgaa cgatttcatc
      481 cctatcgaat ggccctgcgt tccgacatag tttccccatc caaagacagt ggcttgctca
      541 accgataccc gcgcggtgac atcgggggga ataaccgagc gtcgatagtc ttccgattgt
      601 tgttcgaaca attcccagga aggcatactg acgacccgac tttttatccc ttcgagcgcc
      661 aattgctcat gggcttgcac gcacaagggt acctcacttc ccgttccgag caataagacc
      721 tcggggcacc catcgggagg gtccgccaat acataggcgc ctttggccac gcccgacgct
      781 gacgcatatt tcatgcgatc caatgtggga atggcctgcc gcgagacgat cataatcacc
      841 ggttcgtgcc gcaattccat aatcaccttc cacgcttcgg caatttcgtt ggcatctcca
      901 gggcgaaaca ccattaaccc gggaattgca cgaagcgaag agatttgttc aatgggctgg
      961 tgagtcggtc catcttcccc gaccccaatg gaatcatggg taaagatatg cacgacggga
     1021 atttccatca aggcacttaa tcggatagcc ggtctggcat aatcgctaaa gatgagaaat
     1081 cctgagccat aggggcgaac ttttgacaaa gacaggccat tcaaaatagc gcccatcgca
     1141 tgctcccgaa ccccaaaatg gatattccgt cctgatggat tgctgccggt caaatccccg
     1201 gccccctcaa aagttaatcg agtcttcgtg gatggagcca ggtctgcggc ccctccgaaa
     1261 aaccagggaa tagcggccgc cagggcattc aacacgattc ccgacacttc ccgcccggca
     1321 agccccttgg catcaggcgg gaaaacagga actttacggt cccagccatc gggcaattgt
     1381 ctatgttgca tttgatacaa gtggccagcc aattcgggat atttggcttg atagtctgca
     1441 aatcgggcca tccacgcgct ccgcaactct ttgccccgtt tcccgatacc ctgttgaaaa
     1501 tgagccggta cctcatcggg cacaagaaac cgtgcgtctt ccggccaacc ataatagcgt
     1561 ttggctaacc ggatttcttc ttcacccaac ggttccccat gagccgaatg cgtgtcctgt
     1621 ttattcggag ctccatagcc aatatggcta tcgacaatga tcagagtcgg acgctctgat
     1681 tcctttacaa acgtcccaaa agcgcgatcc agcatcttga gatcattcgc atcacccaca
     1741 cgggtcacat tccaaccgta ggcgataaat ctggtagcca catcttcgct aaaggccaag
     1801 tccgtagagc cttcaatcgt gatcttattg ttgtcataga tccaacagag attcgacaat
     1861 ttgaggtgtc cggcaagaga tgccgcttcc gaggacaccc cttccatcaa acacccatca
     1921 ccacaaatgg catacacatt atagttaaac atttcaaaat ccggacgatt gaaatacccg
     1981 gccatccatc gttgcgcaat cgccatgccg acactcgttg cgacaccctg acctaatgga
     2041 ccggtcgtcg tttcgatccc cgatgtccaa cgatactcag gatgaccagg gcatttactt
     2101 tctaattgcc ggaaccgttt aatatcctcc aaggtcaccg aaagctcacc caatcgctca
     2161 tattgcccgt tcaccgattt cacgccacag aggtgcaaca gagaatagag caacattgac
     2221 gcatgcccgg ctgagagaac aaaccggtca cgattcggcc agatgggatc gtccggatca
     2281 gaacgcaaga tccgattcca gagccaatag gcgacaggag ccaaggccat cggcgtaccg
     2341 ggatgcccgg agtttgcggc ttgtaccgca tccatcgata aggtgcgaat ggtgttaata
     2401 caaagctggt ccagggaatc gcatgcgggt ttcattctta ctccttcgtt atccataaaa
     2461 agtgagctat ctcgcgtgac gggttttcaa ttgtgagaac ggtaagtacc tgcatcctat
     2521 cacaggtctt cccttcactc tagattatgg cccactaggt tgttcggcaa acggacttgc
     2581 cgtgaagaaa taaaaaattt tcctcgggcg aagacaaaaa gtcgcataat ttaatcagtt
     2641 gccaatccca tgaacctgtc tttatgattc aaacctatga aagtccatct tagccatccc
     2701 acccgtgacg tggtcatcca aggccccaaa cgggtcgccg acattttcaa agaactcaat
     2761 ttaatccctg aggctttctt gatcatacgc gggcaggatc tcatgaccga agacgaaacc
     2821 gtggcggatg gggatagcat tgaaatccgc ccggtcatat ccggagggta aactcatgcg
     2881 ctgccgaaaa tgtccaaaac gggcggtgct gggactacca aggcacaata ccgcgttttg
     2941 tggggactgt ctcactgaat ttgtccgaac ccaggtgcaa cgagccatta aggcccaaca
     3001 gatgtttgcc cctgaagatc gcgttctggt tgccgtttcc ggaggaaaag acagcctgac
     3061 cctttgggaa attctcctga aattaggcta tcgggtcgat gccttgtatg ttgatttagg
     3121 cattccgggt tattccagcc ggtcaaaaga aaaggtcgaa cagtttgcca aagtggtggc
     3181 tgaaccctgc ggatcccaac tgacgattca taccgtgcaa gaagatgcgg gagccgggat
     3241 taaagaatta gctaatctga tcaagcgccc cacatgctca gcctgtggca cgattaaacg
     3301 gtatcagttt aatcgggtgg cctggcaaaa ccactacgac gtgatggcca ccggccataa
     3361 tttagatgat gaagcggcca gacttctggg caatgtcctg caatggcagg aggaatattt
     3421 gcagaaacag tcgccaaacc ttcccgcctc cgtagaaggt tttgcgaaga aagtgaaacc
     3481 gctctaccga atgaccgaac gggaaattgc cgcctatgcc gtggttaacc ggattgacta
     3541 tttagtcgaa gaatgcccga tggccaaagg cgcaaagatg ttggtatata aagacgcgct
     3601 taatcgctta gaagcggaat ccccgggaac caagcaacgg ttttattggg gattccttga
     3661 tagacaggag aaatcgtcct ctgtggctcc atctatgagc cagatcgacc aaaccacctt
     3721 gcaaccctgt accgtgtgca gccaacccac gactgcggga acctgctcct tttgtcgcat
     3781 gatggcccgc gccaaaacgg cagtgaagta ggctccattt tggcatggcc attctcaatg
     3841 gccttcagaa tatcgaccgc agtttcgcca cgaccccgca gaaacccgct ttcccatgct
     3901 cacctaacat tttggctcaa tcacaaaagt acctagtact tggcttcacg agccgtgaac
     3961 tatttccctg gcttcgtttc ccacatacca aaggagagaa agaggaacta gaaaatttgg
     4021 ccattcgcct aaaaaaggag ggaatttggc gacgtccagt tattcgggga gttcgatact
     4081 acccccttcg aggtcggcat cgatgccggt cagctccatt ttgttcccaa cccgccacca
     4141 ttccggggag atttcagaca aggtgccttt cgaggtataa aattttgccg cagggatggc
     4201 caccgcttga tggggctttt tgtggatttc catgacgagg gtcttcaggg tcgtatcttc
     4261 caccttgacg ctatccggca actccgtaag gacaaaccgg tcgccagggt tgtgttccca
     4321 gacgttgcgt tgcaacttgg ccacttccgt tccggttggc ataaatacac ccatactcaa
     4381 tgccagcttt ttctcctctt ccaaccactc aatacgaagc tgttctttac cgtgggcgat
     4441 aaagacgcca tccgtattcc gaagggtatt ctttcctagt tcaatatcca tcgttaccta
     4501 atcctttctc gggcaactat ccgtgagggt ccgattattc acaatagacc cggagagttc
     4561 aagtcaccga atatttccac agaatcatcc agtttgtcct ctaccgttcc aacgaggtca
     4621 tctggtccgg ctcaccaccc cgacctttca aaaacatcag gcaaacaaga ccaaagacaa
     4681 tccagagtat ttctcgcagt cgtcgaataa gcgcaaaagt gattcccgtc acttccgaat
     4741 aaccaaaagt catcaacagt aaggtatatc ccccttcttg ggcacccagg cttcccggaa
     4801 tgaaaaaggt tccgccctta atgagcacgg ttagcgccgc aattgagagg gctacccacc
     4861 catcgatcct cacaccgaga aagtagagaa tggcatacac ctcaagcgtt tccatcatcc
     4921 atgccaaaaa aaatacggct aacgcggcat aaaaggtctg ccggtgctga gaataaaatc
     4981 cacggatcgt gccgtccagc tcctttagtt gaggttctcg tttttcaaga aaggagaacc
     5041 tgataccaca tgcccgtaat aggccaaggc aacccatacc gagaccgtgg cgttggagca
     5101 gcacaaacaa cccgacgcca aacgctaaga ctcccacact gaccagcatt gccatccagg
     5161 aatgacctga atcgccaagg atccaaaaag ccaccgccaa tcccagcaag ataaaaagca
     5221 cttgggcgaa ggtcatagcg gtcttcgcgg taatcacgga cgccaaccca tccaccattg
     5281 gcaccccgta tcgttttaga agataggcct tcaggggctc cccgcccaca taggccgtgg
     5341 gagtcgtgac attcacagtt tcgccagcca tccggatggc aaataaccgc atgaacccga
     5401 catggcgagt atgagaaccc agagtgagtt gccatcccag ggcttctaac ccgtatacca
     5461 gaatcatggg aaggaggatg agcccgagat gtaggagacc taattgagaa acggtatgct
     5521 gaatcggttc gaggccgatg tggagaacta tcccgaccag ggccgcgagg ccgacgacca
     5581 ggaaaagcat tttaagcacg agggatgaga ctacggcgaa gcatccaggc catcgacatg
     5641 acaaagagcc acgagccaat cgcggccagg gccaaaaacc agtgcaggaa accaaaacca
     5701 gcactcagta acaccatgat ggaaaaatcg cgattggcga cattgcccaa cataaattca
     5761 atattggccc attcttgctc tgtgagttgc cgaatttcac gaggacggga tcgcaattgc
     5821 cgcgcatgat tgactaacag caacgacacg acattcgcta aaaccgctga ggccccaagc
     5881 agaaggggaa ggtgcgaatg ctcccacggc ccctggagaa acgccccgca ggcgatggca
     5941 gcaaacaata caatgtgcac aacattatct gcccaaatat cgagttcttg cccaaacttg
     6001 gactctgaaa acgtcagcct ggcgacctct ccatcgcagc aatcaatgat gacagacaat
     6061 tgcaagatca atccccccag aatggccagt ttccaggacc caggcacaaa aagccccgca
     6121 gccacgagcc ccacgaccat ggaaagcatc gtaatcatgt tcggtgacca tcccaaccgc
     6181 aaaaacccat gcgtgagcag gccggaacat ttacgattca cataccgatc caccaaccca
     6241 tccaagccgc ctttcattgt ttggagtgcc tccaacaggg tccgctcagc gagttttggc
     6301 cccttaggcc cacgaacatc tctgaaccaa tgagaagcgc cgccaatggt ttgaatcgtc
     6361 ccttccactg ccgcttgctc caatgccaac cgaagtggat tcgtgccatt agcatgcaac
     6421 acgcctgaaa tacccaacag ccgggccggg agaaccagaa gatctccaac cagaggcaac
     6481 cggcttcgat caggagtctg acgtttcgtt gaggactgag acgtctggga taccgcctgg
     6541 tcatggaaga ccaccgtcga ggttgaccgt ccctccagac cctcaggccg gaacgccacc
     6601 ccgggatttc cccggtgata tccctcctca ggttgaccaa cgactacgac tgccttccct
     6661 tgagatcctt cagctcgcaa gcgctggatc aatgcgggag agtacacggt atggcaaccc
     6721 acgatcatgc aggctccatt gatttcttcc gcaagggctt cccaggtctg ggggtcatgt
     6781 ggtgggaatt cccgaaccgg cagccaacga acagcggcct gtacccggct atcttcacga
     6841 agtaactgac gaagagcttg ttcttccggc ccagctaaca cccaaatttg agagattccg
     6901 cctcgctgta acgtgaacac cgcccgttga aacagcgtca tgccgccgac acgagtcaag
     6961 ggactgggaa ccgcgtcacc tgtcccaacg gctccgggat caaagacgcc gaccgacgtc
     7021 aataaaattg ccgtatcgac cgcatgggtg gcttcttgtt taagtattcc gtccatacac
     7081 tcattcctac ggtctcagtg tggtggccct ggtattctcc tctaagttta tcacggcggg
     7141 caagatctca gactccgctc gatctatatc ttcaggaaaa tcaatttcta tccacggaag
     7201 ccccccgatt ttttcgtatc ccaccggcac cttctcaaaa aactctttta gcgcgtcctc
     7261 atattccata tcaagcgccc cagctgcaac ataggcctga accgattgca acagtgctgg
     7321 gatatcctgt ttttggactt tgagaaaccc gaccccttcg ccagcctcat cataggcaga
     7381 tggcagggtt ttacttaagg tgaggactcg accagccttc gcggcaatca tacactcttc
     7441 cgattcctgc ttcaccgtct catccatcaa taaggccgtc ggaaagggag aatgaaccaa
     7501 acgagccaga atcgatgggg cgtagagaac atcggcatcc atgagcacga catcatcgtc
     7561 catttcagac cgtgccgccc acaatgacgt tatgctccca cgagtgaact gttcattcac
     7621 cacccatcgg acctcttgaa caaacgggtc agcggccatg gcctttcgaa tatgatcctg
     7681 agcgtacccg accaccacca ccacctggca cacacccaat tgccccagag cttcaacatg
     7741 ccgggataat aaggtccttc cgccaagatc aaccaggcat ttcggtcgcc cctgtgtcac
     7801 accttgcaac cgcttcccga cacccgctgc aaagataatt gctttcacgc tctggctcct
     7861 gaaaggatgg actgaaaagc agagaggaat ccttcaatat tctgattggt cagggcacca
     7921 atattggcaa tacgaaacac tttctcttcc agctgtccct gcccggcata gatcacgtat
     7981 ccggctcgtt ttaattcgtc atgcagccct tggtaggcac acccttcagg cagataaaac
     8041 gcggttaagg tattactttg aagcgtaggg gccagtaccg gcttgacgcc ccactcttcc
     8101 aggcgcgtcc gaatggtggt ggcatatcct ttgaatcgtt gaatccggtt gtcgaggcct
     8161 tcttctaaga gttcattaat cgcttcgcgg aaggcataat agacctgcac ggctggagta
     8221 aagggaatcg tcccccgctc ctgtacttca taataattag ccaaatttaa ataccaggac
     8281 cgcttgggat atttcatcat ccgctccata aagcctttcc ggaccaacac aaacgcggca
     8341 cctggaaaac cttgaataca tttctgagcg gcccccgcaa ccatgtataa tttgtttccg
     8401 gcaatatcca gggcctctcc tcccagcccg ctcacagagt ccaccataaa gacccgatta
     8461 taccgatcca cgacctccgc aacttccttc accggattaa tcaatcccag ggtcgtttca
     8521 tgatgcacca tggcgacggt atgcacttcc ggatgttgct tcagcgccaa atcaaccgtc
     8581 tgcggatccg gtacgattcc ccagtcatac tttaagtccg gcgtacccaa acgttgcatc
     8641 gccagcatgg aagacatccg ttggccatac accccattgt taatcaccat cgctcgcttg
     8701 ccggtaggga tactggacat taacgccgct tccacggcag ctgtgccgga acccgtcaac
     8761 acgatcgcgg tgtactcgtc ttcttcacca ggaacgaaga ccttcagcaa tttttgccga
     8821 atatctgtca ataattccga aaattccgac tctcgatgac agatatccgg ttgaagtaaa
     8881 gcctgacgga ctctttcaga cacattgacg ggaccgggat tgaataaaat catgaatcct
     8941 cttcctttac tttacacgct tacaggctgg cgattaggct ttcacgcgaa tacgtgtgcc
     9001 gttaggtgac actcccctta aaccgtttcg tcagctcagc tggactcaag gcaatccgat
     9061 ccgcatcttc aagctgttca ttcaccttga ccaacaaaaa actcggcccg tcatctccta
     9121 acatggcctt aaactcatac acaatatctt ctcgttccca gactcgctca acattcttgt
     9181 atcctgccgc tttggccatt ttatccaggc cgacaaccct ggaataggtg ggctggtttc
     9241 cggtggttcc atacacctca ttatcaaaga ccacatgaat gagatttttc ggtcgcaacg
     9301 cactaatcgt ggccaacgtg ccaagactca tcaacacatt tccgtcgcca tcaaagacca
     9361 ccaccgtttt gtccggctgg gccagagcca atcctaatcc gatgcccgcc gtcgcgccca
     9421 tcgagccaat catataaaaa ttttgagtcc gatcacgaag tttacaggct tcacgtgagg
     9481 gaaacccgtt acaaacaata agcagttgat cggtcatcag ttccaacatg gccgccatcg
     9541 cctgcgcccg gctctgcatg accccttctt ccggtccaat catcgttgca tctccaacac
     9601 aatggttatt tcttcgagtt cacacccaag ccgttgccca cagaccgggc agaaattgca
     9661 gattggctgc ttttccctta cgagaccaaa tcgggaaatc cgtccagggc cataggacat
     9721 ctcttcaaca tcccactagg gctgaatggt tttcacgatg ccctttttca acaataacgc
     9781 gacagggatc cgcttctcca taaaggtggt ggctgtccat ttcagatcat ctacaatcgt
     9841 ctgctgggac aacgtcctgt ggggaatccg taccgtatcc aaaagcgtcg tcatggtttc
     9901 ccccatcacc agatgttcag gtgcatcctt accttcgaag ccacgccagg ataccaacag
     9961 caaacagggg atctgataaa tcaaattgag agaagccaga acattcaggg cattacctaa
    10021 tccggagttt tgcatcaaca cgaccggaat tttccctccc aggtaagctc ctgcggccat
    10081 cgccacagcc tcatcttcac gaaccgccgg ggtatacaac cgttcttccg tcaaatgggc
    10141 aataatgccc ccaagaatcg tgtccggcac accggtaaaa aaatcgaacc caatgccttg
    10201 caggcctttc acaaaatcgt cagcttccaa taccgtgtta ctgctcatcg ccttagacag
    10261 acctcacaat tccccccgtt ttttcgacac gtaaaacttg tcacacacag aatcggggtc
    10321 agggtaccgt tggatggtgg gagtgttgaa taattaagat tttcaaggac aaatttgccc
    10381 aggattagat tactcatcaa aggctccgtg tcggttcaaa aaagcccgtc cagcaaggcc
    10441 gcagccgttt ttttcgcgcg gagcgtacgc ttagtacgtg agcacggaaa aatggcgaga
    10501 acgccgctgg
//
LOCUS       DATIAL010000076         2212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_873359, whole genome shotgun sequence.
ACCESSION   DATIAL010000076 DATIAL010000000
VERSION     DATIAL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873359"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(599..1219)
                     /locus_tag="VLA60_02505"
     CDS             complement(599..1219)
                     /locus_tag="VLA60_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08253.1"
                     /translation="MGNANLDPARHRIVEPEDQWQPRQVLLFSGHMIDAPGRQPPRFP
                     RDKEVVAAQKIAEVLDQLGAGPDDLALTQGACGGDLLFTEACQQRKVIMQWMQPFHEP
                     EFIQKSVICRGEAWRDRYVAAKAKLTTAIRSAPEHLGSLPEGVDPYERCNLWLLHTAL
                     GYGVEKVHFICLWDGGGGDGKGGTAHMYAEVKRRSGNVTWIDTRTL"
     gene            complement(1311..1997)
                     /locus_tag="VLA60_02510"
     CDS             complement(1311..1997)
                     /locus_tag="VLA60_02510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF08254.1"
                     /translation="MKSLVLPDIEAYAEAHSLPESEMCRRLREETYRNMDCPQMVVGP
                     LEGAFLKVMALSLGARRVLEIGTFTGYSALSMAECLPDDGMVITCDIDPESTAMAKRY
                     WAQSAHGTKIHLRLGPALETMATLTGRFDLIFIDADKANYVNYFRQALELISDHGVIL
                     IDNVLWNGDVLTHPAPDTNTAAIQELNRVVHAEPRVSAVLLTIRDGIFLIKPQSFPRG
                     NGQSQQQVQQ"
     gene            complement(2029..>2212)
                     /locus_tag="VLA60_02515"
     CDS             complement(2029..>2212)
                     /locus_tag="VLA60_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08255.1"
                     /translation="IGKKHSPMSEENRSGVEARDHLTEQRKTEQAKEKKNLKMDEMAC
                     GRRMEQRTMCVIEDQS"
BASE COUNT          466 a          631 c          525 g          590 t
ORIGIN      
        1 cgttttcgat atctttggtt tccttagggt tgattggtca ttacctcctc tgctccgacg
       61 cctgccgtct ggcccaagga agggacgctc tcctcagtgg gcgggccttg tttgagccct
      121 gcgagttgga ccgccctctt cagactggcg tccgttcgct ctcctaaggc cagacggggc
      181 gtccatggtt ttgggtcctt ttgccgaaac aaaagggcct cgcctgccgg ggcgaaaccc
      241 ggcatgtctc ggttaatcaa cgaattcccg gttagtgaaa tgtgagcaca aacctgaata
      301 tttgggttcg tgtaaagcaa aaatgtattg gattttgtag gtacgccctc ttcatcattc
      361 tgaacgtgcc cacacatccc gtcgttcgtt gattccttag atttgtttta ttgccggtac
      421 atattagatg gtcatcacga aactttatcc ctgtcaaagg tcaggatgtt tttacgaccg
      481 ggagattccc ttcgtatttg ccattttcca ttcatgtcgc ctccggcgta ttcgtacaat
      541 taaaccagcg ttttttcagc ctcactgtgt tccgctacga tcacatgaaa aatgttggtt
      601 aaagagtccg ggtgtcgatc catgtgacgt ttccggatcg gcgtttcact tctgcataca
      661 tgtgcgccgt cccgcccttt ccatcacctc cgcctccatc ccacaaacag atgaagtgca
      721 ccttctccac cccatacccc aatgcggtgt gaagcagcca tagattacac cgttcatatg
      781 gatcgacacc ttcgggtagt gagccaaggt gctcaggggc cgagcggatt gcggtcgtga
      841 gcttggcttt ggcggccaca taccgatcgc gccaggcttc gcccctacag atgacagact
      901 tttgaataaa ctccggctca tggaacggtt gcatccactg catgatcacc ttgcgttgct
      961 gacaggcctc cgtgaacaaa aggtcgcccc cacaggcgcc ttgggtgagc gcaagatcat
     1021 cagggcctgc gcccagttgg tctaagacct cggcgatttt ctgtgccgcc acgacttctt
     1081 tatcccttgg aaaacgcgga ggctgtcttc ccggggcatc gatcatatgc ccgctaaaaa
     1141 gcaaaacctg ccggggctgc cactgatctt caggctccac aatccggtgt cttgcggggt
     1201 caaggtttgc attacccatg tttgtctgat ttaccatgtc ctgtgtaatt gccgctggat
     1261 cgggttcgct gagaaacctg tcagtgcgag aagcatttct gggaaatgtg tcattgctgg
     1321 acttgctgtt gcgattgacc gttacctctt gggaaggatt gtggcttaat caggaatatc
     1381 ccatcccgaa tggtcaacag aaccgctgag acccgcggct cggcatgcac cactcgatta
     1441 agttcttgaa tggccgccgt attggtatcc ggagcaggat gtgtcagcac atccccattc
     1501 cacaaaacat tatcaatgag aatgacgcca tgatccgata tcaactccaa tgcctgacga
     1561 aaataattca catagttggc tttgtcggca tcgataaaga tcaggtcaaa ccttccagtc
     1621 aatgtcgcca tcgtttccaa ggcggggccg aggcgaagat gaatcttcgt cccatgagcg
     1681 ctctgagccc aataccgttt ggccatagcc gtggactcag ggtcgatatc gcacgtgatg
     1741 accatgccat catccggaag acattccgcc atggataagg cgctgtagcc ggtaaacgtc
     1801 ccgatttcta atacccgcct ggctccaaga ctcaacgcca tgacctttaa aaatgcgcct
     1861 tccaatggac cgaccaccat ctgggggcag tccatatttc gatacgtttc ctcccgaagt
     1921 cgccgacaca tctccgattc cggcaaggag tgcgcttcgg cataggcttc aatgtccggc
     1981 agcaccaagg atttcatgtt gtcccccctt tgcagtcaat tcatcgcctc acgattgatc
     2041 ttcaatgaca cacattgtcc tttgctccat cctcctgccg caagccatct cgtccatttt
     2101 caaatttttc ttctcttttg cctgctccgt ctttctttgt tcggtcaaat ggtcacgggc
     2161 ttccacgcct gaccggttct cttcactcat cggcgaatgc ttcttgccaa tt
//
LOCUS       DATIAL010000077         9686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1303743, whole genome shotgun sequence.
ACCESSION   DATIAL010000077 DATIAL010000000
VERSION     DATIAL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303743"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(672..902)
                     /locus_tag="VLA60_02520"
     CDS             complement(672..902)
                     /locus_tag="VLA60_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08256.1"
                     /translation="MKCVRCDGLMVVDDCLDIKGAMGELWIKAYRCIMCGNLMDPVIN
                     HHRTDGPVQAQVIPFRKRLRRAPRTPVARLTA"
     gene            complement(1265..2332)
                     /locus_tag="VLA60_02525"
     CDS             complement(1265..2332)
                     /locus_tag="VLA60_02525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSF08257.1"
                     /translation="MNRIDTELGKALKAKIRTQGPMTFRDFMAAALYDETMGFYAKAP
                     KIGSPDGPFDTNARFPAFGYAIAKAITYAEKAIGFHLRVLELGGGTGQLGNNIISCLE
                     NAHEYVVLDPSSGLRDKQKQRGLHAINNIDCLPPGPTFVFGNEVLDALPVHRVMGMGH
                     EEVLELYVGLDEDGEFCEQPSTLSSMELAERLSDDRVQLGRGQVAEICLELKPFLKSI
                     WRVVDPGYVIFIDYGDRASNLYSHRHRNGTLRSYYHQQQVYDPFFAVGQQDLTADVDY
                     TAACFIAEEVGFEVAGPIPQGTWLRNLGILDYKGPTGVRESDQEEIDMLTRPAGLGST
                     FDILIFKTKGLTDGPGLQPAS"
     gene            complement(2492..2845)
                     /locus_tag="VLA60_02530"
     CDS             complement(2492..2845)
                     /locus_tag="VLA60_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08258.1"
                     /translation="MKGIGIFLGTVGASTFALSMAFANPGLLPAHPGYPAAGKSPVTG
                     QATANDPGQTNAGGEKSLLSSQGFGSKAAMNDVSDPNRGRIKKSTGAGRLPEVEGPLN
                     KTNPNPAGAKSTVIK"
     gene            complement(3029..4831)
                     /locus_tag="VLA60_02535"
     CDS             complement(3029..4831)
                     /locus_tag="VLA60_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08259.1"
                     /translation="MSNRLESTLATKQQSTLQKILTSVVNEVGMEAVLVAIMTHDGGP
                     LVEQVSRGFSPREVRAILRALSMEDLKSLAARNGGGGELESVLRIRMVTPGSKVALVL
                     PLKFGGRVYGTLVLARRENSALTKREKTTLSTNVSRISTELKKAGLFGSSLILSKPVV
                     GNEPLALVVAGEEGPAPVARTYSNAEVQERIGSILTEEVDGGLSFDRGWVSIYDPLAA
                     TLEVLGVFGTQKKDVSPGQLLSLDDSASGWSVRHRKPRCDNNLASTQGRFHDYKQLYR
                     DRFASTIVVPFYVRGRVAGTVTLASKNPNNFDQIGSESKSLEAITTKLVELFEDPSCH
                     LSVMDVAPIQPEQPALKGQVITAQPEGGDVRKEERRAALHEVSSFLATEIREPIGFIR
                     AQLEEITTDADLDFDSQTRVENAMRDMIRIETVLNEILDFAKPLELDRKMCRVQDLLD
                     KAFSLVSTDIRVNRIEVLKKVPARLAQVRWDEVKMQHVFLCIFKNAIEAMSPGGHLRV
                     EVMLTRARKPELQIIIANDGVPIPAEFVDKVFEPYFTTKRSGTGLGLATVKKVVEEHQ
                     GQISIASEPDKGTTVTILMPAPRPRTPYRPRRPA"
     gene            complement(4977..5381)
                     /locus_tag="VLA60_02540"
     CDS             complement(4977..5381)
                     /locus_tag="VLA60_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSF08260.1"
                     /translation="MNGFLIRCGITGFAVLLASQIIPGIEITGIASGIAAVVILAFLN
                     SIIRPVLYLLSAPFIFITLGLFMVLINGFLLYLVSILVKGFIVSGFWPAVGGAIIISL
                     VSGVLNLWISEQGRIEIVTHHPSSGRKIRHIN"
     gene            complement(6314..7300)
                     /gene="hpnA"
                     /locus_tag="VLA60_02545"
     CDS             complement(6314..7300)
                     /gene="hpnA"
                     /locus_tag="VLA60_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HSF08261.1"
                     /translation="MKVLITGSSGFIGAAVTRAVVAKGDEVRVLIRPTSNLKNLEGLP
                     VETVQGDLQDPLSLKKAIAGCEGLYHVAAHYALWDQDPSIFYAINVEGTKNLFRAARV
                     SGVQRIVYTSTIGAIGLPDEGGLGNETLFPSLAQLAGDYKRSKFLAEQEVLRMAQEGL
                     PVVIVNPTAPVGERDVKPTPTGQIIVDFMRGRMLAYIETGMNLVDVEDVAIGHIRAME
                     RGRVGERYILGNQNLTLKEVCQMLSRLTGVSAPRLQLPWRLVLPMAHVNQWIANLVTH
                     RPPRIPLEGVRMAKYHMHYDCTKARRELSLPQTPVETALKKAVQWFRQHGYG"
     gene            complement(7431..8321)
                     /locus_tag="VLA60_02550"
     CDS             complement(7431..8321)
                     /locus_tag="VLA60_02550"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HSF08262.1"
                     /translation="MNIQAYLDQQRQRVDRFLEKSLPLPSANPQRLYESMQYSLLGGG
                     KRIRPILTIAAAQALGYDRDAILPFAASLEFVHTYSLIHDDLPAMDDDDYRRGRLTNH
                     KVFGDGMAILAGDALLTMAFELCSQVDATEGFSAAQQLAIVRELAKGSGHQGMVGGQV
                     MDIQAENQEIDLAHLQQIHTFKTGRLIRAAVRIGSIIGGATTQQMQCLTDYSEDIGLA
                     FQIADDVLDMVGTREELGKDAGTDEKRGKRTYPSFFGIEGARQLGEECVQRAIARLNT
                     FDKQADPLRHIATYIMARRS"
     gene            9178..9357
                     /locus_tag="VLA60_02555"
     CDS             9178..9357
                     /locus_tag="VLA60_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08263.1"
                     /translation="MKNVELTVNGTMLTITVDLSKDFGPSSSGKTTIIASTEGNVSIP
                     DREEKIGLNIYKKKT"
     gene            complement(9364..>9686)
                     /gene="uvrA"
                     /locus_tag="VLA60_02560"
     CDS             complement(9364..>9686)
                     /gene="uvrA"
                     /locus_tag="VLA60_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247168.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HSF08264.1"
                     /translation="LSKELSKRSTGRTLYILDEPTTGLHFVDIQRLLDVLDRLVENGN
                     TVLVIEHNVDVIQNADWVIDLGPEGGDQGGCVVAEGTPQSVMKVKASWTGQVLLEDFK
                     NRSS"
BASE COUNT         2625 a         2582 c         2117 g         2362 t
ORIGIN      
        1 ccatcgttcc cagataaaga gcaccaacag gattcccatt aggcccagga aaaagaggtg
       61 gtcactgttc acaaatgaga tgaatcctct atcagggcat caatgatggg ggtgggaagg
      121 gggagggaag ctgccaaaac actcctaaaa tctataacta tacgccattc atggcatgct
      181 caggtcattt ttagacttct taaacaacat agcgtatcaa cattgggaat gaaatgtcct
      241 acaaccccca aaccgcaggg ggaaaatagc attcacggac tggttgggat tctctcggaa
      301 aaggtgggcc caagcatgcc gagctgaatg ccggcttgga gatcgcccat atccccatgg
      361 ggggcatgtc taataaataa ctcaaggaag aattgtcagg caaaatcagg cagtcatgcc
      421 agaatagccg cagatgaaaa attggcagag tttcgaggaa aggactatac caaggccatg
      481 tgaataagaa agttaaatct aaacacaccc gattcaaaaa cgccggaaga accgggaatt
      541 taatctattg gtgccagatc ctggccacag cggagcacga gaacttcctc ctgagccagg
      601 agtcaatcgg tacctgggag agtttttcct cccaagtcgc acagaatctc tcaaaatttt
      661 cgcccgccct tttaggccgt caatcgagca acaggagtcc gtggagctct tcgtagtcgc
      721 ttccgaaatg gaataacctg cgcctgaacc ggaccatcag tgcgatgatg attaatgacg
      781 gggtccatca ggttcccgca cataatacat cgatacgctt taatccaaag ttctcccatc
      841 gcgcccttaa tgtccagaca atcatcaacc accataagcc catcacatcg tacacacttc
      901 ataactctcc ctcctttgaa agcctcctgc ttcccactct tctcaacagg tgagtcagtg
      961 catcacgcgt gccacgcacc acgtgagatc tcgaaaagca ccacttaatt aggtgtgtat
     1021 gattgcggaa aatgaaagga gatcactaga taatcgaatg aatttttgga gtctacaata
     1081 ttttgtgata aaaaaaaaga gcaaacacac attttgtggg tgctggaaaa attgactaaa
     1141 gtttaggcat catgctcgtt tttagaaaaa ttatttatct ctcaccacag tccccttcgc
     1201 ctatgtcggt tcaaataaaa atatggtgag atttggcagg ctagtggaaa ttttgacagg
     1261 tcctttatga agccggctgt agacctggcc catcagtgag gccctttgtt ttgaatatga
     1321 gaatgtcaaa agtacttccc aggccggccg gcctagtgag catatcaatc tcttcttgat
     1381 cagactccct aacacctgtt ggccctttat aatccagaat tccaagattt ctcagccatg
     1441 ttccttgcgg gataggccct gctacctcaa aaccaacttc ctctgcgata aagcaagcag
     1501 ccgtataatc cacatctgcc gtcaagtctt gttggcccac agcgaaaaac gggtcataca
     1561 cttgttgctg gtgatagtat gaacgcagcg tcccatttct atgccgatgt gaatataaat
     1621 ttgaggcgcg atcaccataa tcgataaaaa ttacataccc tggatccaca actctccata
     1681 tgcttttgag gaatggtttc aactccaaac aaatttctgc gacttgccct cgcccaagct
     1741 gcaccctatc gtcactcaac cgctcggcta gttccataga tgaaagagta ctgggttgct
     1801 cacaaaactc tccatcctca tccagaccca catacaattc caatacttcc tcatggccca
     1861 ttcccattac tcgatgaacg ggtaacgcat caagcacttc gttcccgaac acaaatgtcg
     1921 gtcctggggg taaacagtcg atattgttga tcgcatgcaa tcctctttgt ttttgcttgt
     1981 ctcgcaaccc agagcttggg tctagaacga catattcatg agcattttca agacaagaaa
     2041 taatattgtt ccccaactgg cctgttcccc cacctaattc caagacccgc aggtgaaaac
     2101 ctatcgcctt ctctgcatag gtgatggctt tggcaatggc atatccgaaa gccgggaatc
     2161 ttgcattggt gtcaaatggc ccatcaggac tcccaatttt gggtgccttt gcataaaacc
     2221 ccatagtttc atcatataaa gctgccgcca taaagtccct gaaggtcatt ggcccttgcg
     2281 tacgaatttt ggcttttaag gcctttccaa gctccgtatc aatccgattc aaggtaagtc
     2341 caagctaaat gtgttgaggg tgacagtgaa aaaaggcatg gaaggaggta ggagcagatc
     2401 atgaaaaatg atgggaaaaa aatgtcacac aaaaaaaaag ggtgccattg ctggcaccct
     2461 tctcctgagt attcacactg taaaaagtaa gttacttaat aacagtcgat ttggccccag
     2521 ccggattcgg gttggttttg ttgagtgggc cttcgacttc aggcaaccga ccggcaccag
     2581 tcgacttttt gatccggcca cgattaggat cactaacatc attcatagca gctttgcttc
     2641 caaaaccttg agaagacaac agactctttt ctcctccagc attcgtttgg ccaggatcat
     2701 tagccgtagc ttgaccggtc accggagact tcccagcagc cggatacccc gggtgagctg
     2761 gtaacaaccc tggattggca aaggccatcg atagggcgaa ggtgctagca cccaccgttc
     2821 caagaaaaat tccaatccct ttcataagac cgctcctttg ttggttatga ctggttaaca
     2881 atagaaaatg gtctggatat gtacatagcc caatttattc aaatagaaaa ggcgatctta
     2941 ttcagattgg ccggtagtgt caagcttacc caaccaaaat ggctaaaatt tcgaaatggc
     3001 accgcttcag ggactttctt tggttccatc aagctggacg gcgtggccga tacggagttc
     3061 ttggtcgtgg agctggcatg agaatagtga cggtcgttcc cttatctggc tcgctagcga
     3121 tactgatctg cccctggtgc tcctcaacga cttttttcac ggtcgccaaa cccaatccag
     3181 tccctgatcg ctttgtggtg aaatagggtt caaacacctt atccacaaat tcggccggta
     3241 tgggcactcc atcattggca ataattatct gaagttccgg tttgcgagct ctagtgagca
     3301 tgacttccac ccgcaaatgc cctcccggag acatggcttc aatggcattt ttgaaaatac
     3361 acaggaatac atgctgcatt ttcacttcat cccaccggac ttgggccaac cgagccggca
     3421 cttttttgag gacttcaatc cgattgacgc gaatgtccgt cgacaccaac gaaaaagcct
     3481 tatccaataa atcctgaacc cggcacattt ttcgatcaag ttccaacggt ttggcaaagt
     3541 ccagaatttc atttaagacc gtttcaatac gaatcatatc ccgcatggca ttttccaccc
     3601 gggtttgcga atcgaaatca aggtcggcat cggtcgtaat ttcttctaac tgagcccgga
     3661 taaacccgat aggttcacga atttctgtag ccagaaacga gctcacttca tgcagtgcgg
     3721 cacgtcgctc ttccttacga acatcccctc cctccggttg agcagtaatc acctgccctt
     3781 tgagagcagg ttgctcgggt tgaatcgggg caacatccat gacggagaga tgacacgacg
     3841 ggtcctcaaa caattccacc aatttggtcg taatggcctc aaggcttttc gattcgctcc
     3901 caatttgatc gaaattatta ggattttttg acgctaacgt gaccgtaccc gccacccgtc
     3961 ccctgacgta aaaaggaacg acaatggtcg acgcgaaccg atcacgatag agctgtttat
     4021 agtcgtgaaa acggccttgg gtcgaagcca aattattgtc acagcgaggt tttcgatgac
     4081 gaacagacca cccggaagcc gaatcatcca gagagagaag ctgcccgggg ctcacatcct
     4141 ttttctgagt tccgaataca cccaacacct ccagtgtcgc ggcaagaggg tcatagatgc
     4201 tgacccagcc acgatcaaaa gacagccccc catcgacttc ttctgtcaaa atgctcccta
     4261 tccgctcttg gacttccgca ttcgaatacg ttcgagccac gggcgcaggc ccctcttccc
     4321 cagccacaac caatgccaaa ggttcattac ccactactgg cttgctcaat atcagtgagg
     4381 aaccaaatag tcccgctttc tttaattctg tggaaatacg cgagacattg gtggaaagag
     4441 tcgtcttttc tcttttggtc agcgcggaat tttctctcct ggccagtacc agcgtgccat
     4501 atactcggcc gccaaacttt aagggaagga ccaacgcaac cttgctccca ggagtcacca
     4561 tgcgaattct gaggacgctt tccagctccc cgcccccccc attacgggcg gccaaacttt
     4621 tcaaatcctc cattgaaagt gcacgaagaa tcgcgcggac ttctcttgga gaaaaacccc
     4681 gagaaacctg ctcgaccaat ggtccaccat catgcgtcat aatcgcgacc aagaccgctt
     4741 ccattccgac ctcattgacc acggaggtca gaatcttttg aagggtactt tgttgcttgg
     4801 tagctaatgt ggattcaaga cgattggaca tgcatgcgtt tcctctcaga atatttgcgc
     4861 acaatgaatt gtcggatatg ccgatggtta aggagcgaag ggttttttcc ccagccctcg
     4921 aaaaattcct gaacctctcc tcaacaccat aatgaaatat ccaaggagtt gtcccgttaa
     4981 tttatatgac gaatttttcg acccgatgaa ggatgatgcg tgacaatttc tattcggcct
     5041 tgctcggaaa tccagagatt taatactcca ctaaccagac taataatgat cgcgccaccc
     5101 accgctggcc aaaatccaga gacaataaat cctttcacca gaattgatac caaatagagg
     5161 aggaatccat taatgagaac catgaatagc cctaatgtta tgaaaataaa tggggctgaa
     5221 agtagataaa gcacggggcg gatgatggaa ttgagaaatg caagaatgac cacagctgca
     5281 atccccgagg caatccctgt gatttcaatg cccgggatta tctggcttgc caacaatacc
     5341 gcaaaccccg ttatcccgca tcgaattaga aatccattca tgtttgcatc ataccggccc
     5401 tgcggtagtg gcctcaagcg acacaacccg caatggggtc aaaaattctt ggggatgccc
     5461 cttcctcccg cctcaagact cggcaattct ggatttgaac catcctcgcc acaatgaagc
     5521 gagaagaatt gcggtgagca ggaggtaaat aaagcaaccc tctatgccca tcagcatttc
     5581 ctcaattcca gaagccccac aaatttaaga ccaggatgcc atactagaga ccaataccta
     5641 atagcaattc ccatttcacc acctcacgag aaatggggtc tgttgcgttt cggctcggct
     5701 ccacccaacg atacccaaaa tgagagagga atccgacgac cgtccatccc gcaaaattgg
     5761 caagaggaac accacaataa attcccttct agggctgccc aaaaatattt tccaagatcc
     5821 atccttcacc cggaaggcct actggatcgg ttaccacatc cgcaaaatta aaacacacca
     5881 ccgttaaacc caacccaggc catgaggttt ttaatcccgg aacgaatagc cacccttttt
     5941 ggcccatccc ttttcccacg gattcacacc aatcagagcc aaagagtcgc cgccaaacaa
     6001 taaagatgag aatgaaaggg aatccatgaa gggaatgctg gaaagataaa actcatgatc
     6061 tgttgagccc gtataaaaat actcgcctta gggattccga tccgggtcga ggcataatca
     6121 caaataaaag caattgcctg cgtgaggccg aaaaacctgc ctgtcagccg gtatccgaag
     6181 atagtttccc aaacagagaa tggcactaaa aagacaaaag catcgggcat gagatcccaa
     6241 tgtgggcgag cagaaaaata gattccacaa tttggctttg taacggtgat gggcaaagct
     6301 tgcgaaggag agatcagccg tatccgtgct ggcgaaacca ctgtacggct tttttgagtg
     6361 ctgtttccac cggggtttgg ggaagcgaca gttcccggcg cgccttcgtg caatcataat
     6421 gcatgtgata cttggccatc cgcactcctt ccaaaggaat tctgggtgga cgatgggtca
     6481 ccagattggc aatccattga ttcacatgcg ccatgggaag gacaagcctc caaggcaatt
     6541 gaagccgcgg ggcagatact cccgttagtc ggctgagcat ctgacatact tccttcagtg
     6601 tgagattttg atttcccaaa atataccgct cacccacccg accgcgctcc atcgcccgga
     6661 tatgccctat ggccacatct tcaacatcca ccaaattcat cccggtctca atatacgcca
     6721 acattcggcc acgcataaaa tctacaataa tttggccagt tggagtgggc ttgacgtcgc
     6781 gctctcccac tggagccgtt ggattgacaa taaccaccgg caacccctct tgagccatgc
     6841 ggaggacttc ctgctcggcc aaaaattttg atcgcttata atcccctgcc agttgggcca
     6901 aggaaggaaa gagggtttca tttccaaggc ctccttcatc aggcaacccg atcgcaccaa
     6961 ttgtgctagt gtagacgatt cgttgaacgc ctgataccct tgctgcacgg aataaatttt
     7021 tggtcccttc gacattgatg gcataaaaga tagaagggtc ttgatcccac aacgcataat
     7081 gcgccgccac gtgataaagc ccttcgcatc cggcaatagc tttcttgagg gacaagggat
     7141 cctgcaaatc tccctgaacg gtttcgacgg gtaacccttc aagatttttc agattactgg
     7201 tgggccgaat gagtacacgg acctcatcgc ctttcgcaac gactgctcgt gtcaccgcag
     7261 ctccaatgaa acccgaagac cctgtaatga gtactttcat aaaaatctga cgttaccaag
     7321 gcgctcaata acaataccgc atgacctgta ccaaccaaac tgttgtcaat gagaaggggt
     7381 gggaaactgt gtaatgtcgc ttcaagaccg cagcgccata cgagagagaa tcaagaccgt
     7441 cgcgccatta tatacgtggc aatgtgtcgt aaggggtcag cctgtttatc aaaagtattc
     7501 aatcgggcta tcgcccgctg gacacattcc tctcccagtt gcctcgcgcc ttcaatccca
     7561 aaaaatgaag gataggtccg cttccctctt ttttcatccg tccctgcatc ctttcccaat
     7621 tcctcccgtg tccctaccat gtccaacaca tcatcggcaa tctgaaacgc cagcccaata
     7681 tcctcggaat agtcggttaa acattgcatt tgctgggtcg tggcccctcc gataatgctg
     7741 ccgatccgga cggcggcgcg aatcagacgc cctgttttaa aggtgtggat ttgctggagg
     7801 tgagctaaat caatctcctg attttcagcc tgaatatcca ttacctgtcc gcccaccatt
     7861 ccctggtgcc cagaaccctt tgccagttcc cgcacaatgg ctaactgctg agcggccgag
     7921 aaaccctctg tggcatcaac ttgactacat aactcaaacg ccatcgtcaa caatgcatca
     7981 cctgccaaaa tcgccatgcc atcgccaaat actttgtgat tggttaaccg gcctcgccga
     8041 tagtcatcgt catccatcgc cggaagatca tcatgaataa gggaataggt atgaacgaac
     8101 tccaaagaag cagcgaacgg caagatggcg tcacggtcat accccagcgc ttgggcagcg
     8161 gcaatcgtca aaatcggacg aatgcgtttt cctccaccca gtaggctata ctgcatggac
     8221 tcatatagtc gttgaggatt ggccgagggc agaggaaggc tcttctccaa aaaccggtca
     8281 acccgttggc gctgttggtc taaataggct tgaatgttca caaaggagcg aatagacgac
     8341 gtggaaggat ggatggaatc gttggtgacc ctatcaaatg gatttttgga gtgtcaaatt
     8401 gtgaacctaa aacctaggga tatcggctcg gtaaacggtt cccaaaagtt ttcttcattg
     8461 ttttcatctg aattactcag tatacttttc ctctatgagc attcgaaatc gagccggaga
     8521 ttgggagccc ctgttattgg gtatcgaacc cgaaatgtgc ggcgtatgtc acgaaggcct
     8581 gtaccggaac cttaccatgc tccgtggagg atggtctcgt tgcagcatct gtgaacattt
     8641 cgttcattat agttgcctgg ctagcggaaa ggtctcctat ctgaaaagac gcccgcgtgt
     8701 gtgcaaaacg tgtgcagacc agcagaccat cccccaacct gccatatcca cgccttcggt
     8761 gcctgccgaa gtcaaataat ggaacatcca gggatttcaa ccccacaagc tatgggctct
     8821 cctcgacttt cagcatcttt tctccatttt tttctggccc ccatcctttg tgcatttagt
     8881 ggttttcata cagccagcta tctgttcgtt ggtctctaca cgtggcctcg aaccagccgg
     8941 gtgcttgtcc tgaccattgg tatgcttatt ttctcatatg aatttattta taagaaccat
     9001 ttgtctcgcc ctacttctac atggaaatat tcctctctgg aatcggtatt ctatacctgc
     9061 gttcttccct atatcatcgg cgcaggagcg ctcttggtcc tcgcgggcct gtcagagtag
     9121 ggcaggaatt ctgccagcac ccttcaacct gcattgatct gcactgagga aataaccatg
     9181 aaaaatgtgg aattgactgt caacggaacg atgctaacaa ttacggtaga cctgtcgaag
     9241 gattttgggc cttcttcatc gggcaaaaca acgattattg cctcaaccga aggcaatgtc
     9301 tcgatacccg atcgagaaga aaaaatcggg ctgaatattt acaagaaaaa aacctagcat
     9361 tcctcaggaa gaccggtttt taaaatcttc tagcagcacc tgccctgtcc acgatgcctt
     9421 gaccttcatc acggattgcg gtgtgccctc ggccactaca caccctcctt gatctccacc
     9481 ttccggcccc aaatcaatga cccaatccgc gttctgaatc acatccacat tatgctcaat
     9541 cacgaggacc gtatttccgt tttccactaa ccggtcgagc acatccagca accgttgaat
     9601 atccacaaaa tgcagtcccg tcgtcggttc atccaaaata tagagcgtgc gaccagtgga
     9661 tcgtttcgag agctccttgg aaagct
//
LOCUS       DATIAL010000078         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_336240, whole genome shotgun sequence.
ACCESSION   DATIAL010000078 DATIAL010000000
VERSION     DATIAL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336240"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /gene="meaB"
                     /locus_tag="VLA60_02565"
     CDS             complement(<1..667)
                     /gene="meaB"
                     /locus_tag="VLA60_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HSF08265.1"
                     /translation="MSGSNPSTVPEYSTIPSLLKEVRTGNIRAISRAISLLETNGPER
                     KAVLDGLRPFPGHACVIGITGYPGAGKSTVIDQLITAYRSQGKKVGVLAVDISSSLTG
                     GAILGDRIRMQHHSLDERVYIRSMGTRGHRGGLAIATRDAIHVLDAAGFDIVLIETIG
                     MGQEEGEIAQIAQTVVDVVAPGLGDDVQAMKAGIFEIAHIVVVNKADHAGAHETLQSL
                     REWV"
     gene            complement(636..1133)
                     /locus_tag="VLA60_02570"
     CDS             complement(636..1133)
                     /locus_tag="VLA60_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HSF08266.1"
                     /translation="MKHSETTRKPMEKRQETNLPEGAPLIIQDHYEIDYRHSYAQDSP
                     FFVGLSQGRLLGSRCTGCRYMYATPRAHCMECGAPTTWEALPPTGRVHTFTICYYGGE
                     SFLKETPFLLVLAEFDQVNTLFLSRLKGVPPEEVHIGLPIRPQFSDTPTFKVTDVWFE
                     PLDRT"
     gene            complement(1141..2424)
                     /locus_tag="VLA60_02575"
     CDS             complement(1141..2424)
                     /locus_tag="VLA60_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08267.1"
                     /translation="MRPVYMISGGITKFAKAHPDKDFRLMVKEAYDAALQDVPDLSKD
                     MIDGGVGSYFSDHFTRQLKAASMAHDYLGLCPKPSKRVEGGGATGGLCFQSAWEAIAS
                     GRMHCCVAYGFETMSRVNTWKGNEFIALASDTNFDFPVGGFYSGYYAMMVNRHMHEFG
                     TTVEQMAMVSVKNHLNALYNPYAQKAKRLTVKQVRESPMVATPLTMEDICTMSDGAAV
                     CILADETIAEQVCDRPVHITGIGSGSDAMRMADRPHGKVPLLPHEQESDYARLKYPGV
                     HSFRGGRMAAKLAYEMAGIRNPLEELSFVELHDAYTSSEIQTYEDLGLCKYGEGGSFI
                     EKGIPFMPGIEYGLPLPDQGRFPVNPSGGLLACGHPVGATGLMQAVFAFWQLQGTIEK
                     HLGSPALQLRDPRRGLIHSHAGTGTYITVSILERT"
     gene            complement(2421..2822)
                     /locus_tag="VLA60_02580"
     CDS             complement(2421..2822)
                     /locus_tag="VLA60_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSF08268.1"
                     /translation="MTTATTPIRVLIGKVGLDGHDRGVKLVARALRDSGVEVIYTGLH
                     QTPEQVVNIAIQEDVNAIGLSIHSGAHTSLFPRVLELLKTHHAEDIAVFGGGIIPSDD
                     VGPLMETGVRALFPPGTSMSRIVEFVKGLPR"
     gene            complement(2819..3652)
                     /locus_tag="VLA60_02585"
     CDS             complement(2819..3652)
                     /locus_tag="VLA60_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF08269.1"
                     /translation="MYPVTDGRFRLDGGAMFGVVPRVLWEKCCPADDHHRIPLSLTCL
                     LIQAHGKNILVDTGLGSKQDKKFHRMFAVDQSSSLHDALHHFRLAPKDIDMVINTHLH
                     FDHAGGNTRRNDKGQTIPTFPMARYLVQRGEFEDAIKANERTKASYRQENFIPVLEHH
                     QCEFVTGTTEWLPGVSMVVTPGHTKYHQSVKIESEGHTAFFLGDCIPTVSHLPLPYIM
                     GYDLFPLQTLETKRWILDQAYEEGWLLIFEHDPRIAMGYVHKDPEGKYFLKPAEEART
                     A"
     gene            complement(3821..>4092)
                     /locus_tag="VLA60_02590"
     CDS             complement(3821..>4092)
                     /locus_tag="VLA60_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HSF08270.1"
                     /translation="YVESEQYTIPTLRIGKEVEQYQRERLRSFRASRDPKTVAEALHE
                     IQWFAQSGENLLPHLMHAVKAKATLGEMCGALKGVFGTYREPVVL"
BASE COUNT          898 a         1106 c         1107 g          981 t
ORIGIN      
        1 gcacccattc ccgcaacgac tgaagggttt cgtgggcgcc agcatgatcg gccttattga
       61 cgacgacgat atgggcaatc tcaaaaatcc ccgccttcat ggcctgcacg tcatcgccaa
      121 gaccgggagc gacgacgtct accactgttt gggctatctg ggcaatttca ccttcttctt
      181 gtcccatccc gatggtttca atcagcacga tatcaaatcc ggccgcgtcg agcacgtgaa
      241 tggcgtcccg tgttgctatg gccaggccac cccggtgtcc tctcgtgccc atgctccgga
      301 tgtagacacg ctcatccaac gaatggtgct gcatacgaat gcgatctccc agaatggcgc
      361 cgccggttaa tgagctgctg atatccacgg ccagcacgcc gacctttttc ccttgtgagc
      421 gatacgccgt aatcaattgg tcgatcacag tactttttcc cgcaccggga tacccggtga
      481 tcccgatgac gcatgcatgg cccgggaaag gcctaagccc atccaggacc gcttttcttt
      541 caggcccatt cgtttccagt aatgaaatgg cgcgggatat tgcccggatg tttcccgttc
      601 ggacttcctt gagtaaggaa ggaatcgtgg aatattcagg tacggtcgag gggttcgaac
      661 cagacatcgg tcaccttaaa agtgggggtg tcggaaaatt gtggacgaat cggcagtccg
      721 atgtgaacct cttccggtgg cacgcctttg aggcgggaga gaaagagcgt attcacctga
      781 tcaaattccg ccagcaccaa cagaaagggg gtttctttca gaaaactctc cccgccgtaa
      841 tagcagatcg tgaaggtatg cacgcgtccg gttggcggaa gtgcttccca ggttgtgggc
      901 gccccgcatt ccatacaatg tgcccgtggt gtcgcataca tataccggca ccccgtacat
      961 cgtgatccca gcaagcggcc ttgactcaaa cccacaaaga agggggaatc ctgggcataa
     1021 ctatgccggt agtcgatttc gtagtggtcc tggatgatca acggggcgcc ttccggcaga
     1081 ttcgtctcct ggcgtttctc catgggtttg cgggttgttt cagagtgttt catggtgtgg
     1141 ttatgtcctc tccaagatcg acacggtgat ataggtcccg gtgcccgcat gactatggat
     1201 gagtccacgt cggggatcgc gaagttgtaa tgccggactg cctaaatgct tctcgatcgt
     1261 gccttgtagt tgccaaaacg caaagacggc ctgcatcagg cctgtggctc cgaccggatg
     1321 gccgcaggcc aacagtcccc cggatggatt cacgggaaat cgcccttgat ccggtaacgg
     1381 caagccatac tcaatccccg gcatgaacgg gatacccttt tcaataaaac tgccgccttc
     1441 cccatattta cacaacccca ggtcttcata ggtttgaatt tcagaggagg tataggcatc
     1501 gtgaagctca acaaagctta attcttcaag tggattccgg atgccggcca tttcgtaggc
     1561 gagtttggcc gccatgcgac caccgcgaaa ggaatgcacc ccggggtatt tgagccgggc
     1621 ataatcggat tcctgctcgt gcgggagcag cggaactttt ccatgcggcc ggtcagccat
     1681 acgcatggca tccgaccctg atcctatgcc ggtgatgtgg accggccgat cacagacctg
     1741 ttccgcaatg gtttcgtcag ccagaatgca gacggctgct ccatcggaca tggtgcagat
     1801 atcttccatg gtcagtgggg tggctaccat gggcgattct cgaacctgtt tgacggtgag
     1861 acgttttgcc ttctgcgcat aaggattgta cagggcattc agatgatttt tgacggagac
     1921 catggccatc tgttcaaccg tggtgccaaa ttcatgcata tggcgattga ccatcatggc
     1981 atagtacccg ctgtaaaacc cgccgaccgg aaagtcgaaa ttcgtatcgg aagccaacgc
     2041 aataaattcg ttgcccttcc aggtgttgac ccgggacatg gtttcaaatc catatgccac
     2101 acagcaatgc attcgtccag aggcgatcgc ttcccaggcc gattgaaaac acaggcctcc
     2161 cgtggctccg cccccttcga cccgttttga cggtttaggg cacagtccca ggtaatcgtg
     2221 tgccatgctg gcggctttga gctggcgggt gaaatgatct gaaaaatagg agccgacccc
     2281 gccatcaatc atgtctttcg ataagtcggg cacgtcctgg agggcggcgt cataagcttc
     2341 tttgaccatc agacgaaaat ctttatccgg atgggctttg gcgaatttgg tgatcccacc
     2401 actgatcata tagaccggtc tcatcggggc agtcctttca cgaattccac aatgcgtgac
     2461 atcgaggtgc cgggagggaa caaggcccgc acaccggttt ccatcagagg gccgacatca
     2521 tcagagggga taatgcctcc accgaagacg gcgatatcct cggcatgatg cgttttcagt
     2581 aattccagta cccgtggaaa cagagaggta tgggcaccgg aatgaatgct gaggcctatt
     2641 gcgttcacgt cttcttgaat ggcgatattg acgacctgct cgggggtttg atgtaacccg
     2701 gtataaatga cttccacgcc ggagtctcgc agggcccggg ctaccagttt cacaccgcgg
     2761 tcgtggccat ccaaaccaac ctttccgatc aggactcgaa tgggcgtcgt cgctgtggtc
     2821 atgctgttct cgcttcctct gcaggtttca agaaatattt gccttccggg tctttatgga
     2881 cgtaccccat tgcaatccgg gggtcatgtt cgaaaatcag gagccatcct tcttcgtagg
     2941 cttgatcaag aatccatcgt ttggtttcca gcgtctgtag cggaaagagg tcatagccca
     3001 taatgtaggg gaggggcagg tgagagaccg tgggaataca atcacctaaa aagaaggccg
     3061 tgtgtccttc ggattcaatc ttgacgcttt ggtggtattt ggtatggccg ggggtgacta
     3121 ccatcgagac gcccggcagc cactcggtcg tgcctgtgac aaactcacac tggtgatgtt
     3181 ccagaacagg aatgaaattt tcctgacggt aactggcctt ggtccgttca ttcgccttga
     3241 tggcatcctc aaattctccg cgttgcacca gatagcgggc catcggaaac gtcgggatcg
     3301 tctgtccttt gtcattacgc cgcgtgttgc ctccggcatg atcgaaatgg agatgagtat
     3361 tgatcaccat atcgatatcc ttcggggcaa ggcggaagtg gtgtaaggca tcatgaagcg
     3421 aggaggattg atccacggca aacattcgat gaaatttttt gtcctgtttt gagcccagtc
     3481 ccgtatcgac gaggatgttc ttcccatgtg cctggatcag cagacaggtc aagctgagcg
     3541 ggatgcgatg gtgatcgtcg gccggacaac atttctccca gagcaccctg gggaccaccc
     3601 cgaacatcgc tccgccgtca agccggaacc ggccgtctgt cacaggatac atgttaaatt
     3661 gtccaagttt cacaggattg gtcccttctg ttttgaggaa agggttttcc ggctacagga
     3721 acattggtga aaactgtatg ccggttttgc tctgttacct ccttgtgaat tgggtcctaa
     3781 aggccaacat ggcttgtggt cctcaggctg cggcaacagt tcataacacc accggttcac
     3841 gataagttcc aaagactccc tttaaggctc cacacatttc tcccaatgtc gccttggctt
     3901 tgacggcgtg catcaggtgt ggcaacagat tttcgccaga ttgcgcgaac cattgaattt
     3961 catgaagcgc ttcagccact gtcttgggat cccggctggc gcgaaatgac cgtaagcgtt
     4021 cccgctgata ctgttcgact tccttcccga ttcgcagggt gggaatggtg tactgctccg
     4081 actcaacgta ct
//
LOCUS       DATIAL010000079         2774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_228714, whole genome shotgun sequence.
ACCESSION   DATIAL010000079 DATIAL010000000
VERSION     DATIAL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228714"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VLA60_02595"
     CDS             <1..524
                     /locus_tag="VLA60_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HSF08271.1"
                     /translation="YAIRQMPPLSLGPLRRKKLYLMGHDVSLIEQVAREYVELFVIPR
                     VSTRALSRLVRHQEAGHVVAFVSGSPECLVRPLAAKLGVSLVFGARLETQAGMYTGNV
                     FAPLPYGEGKGRHVERLAARYNLDLSRSYAYGDSPGDFHALQLVGHPFVVNPIRGMAR
                     IAHRQGWPITQWA"
     gene            602..1255
                     /gene="queE"
                     /locus_tag="VLA60_02600"
     CDS             602..1255
                     /gene="queE"
                     /locus_tag="VLA60_02600"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HSF08272.1"
                     /translation="MATLPLLNITEIFHSIQGESTRVGQPCVFVRLTGCPLRCTWCDT
                     AYAFYGGTSMPMEAILAQVRSYGCSLVEVTGGEPLHQPGSLVLLTELCEAGFQVMLET
                     SGACDISQVDTRVSIILDVKCPGSGMTDRMRWDTLTHLSRKDEVKFVLKDRADYEWAR
                     DIIQQYALGSRCPVHLSPVFGALHLQSLAEWILEDRLPVRFQLQLHKVIWDPQTRGV"
     gene            1312..>2774
                     /locus_tag="VLA60_02605"
     CDS             1312..>2774
                     /locus_tag="VLA60_02605"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSF08273.1"
                     /translation="MNIQVKSGEATTAATDMLVCLEYEQEKNWSKALRPVDRKLGGQL
                     SDLRKSGEFSGKPNNMALLHIDGKLSAKRVLLVGLGPRETVTLERIRQAMGTAAKRAR
                     SVKAKGIVSVMPDVPKAAGHMTDVAQAMVEGLILGDYRFNEYRTDRSDDNHTLQSCTV
                     LAPSSSSMSEVKEGTNRGKILGDATCFVRDLCNHPANVMTPSRVVTEARKIAREAKIR
                     LKVFDRRQQEKLGMGGLLGVSRGSIEPPQFIILEYAGGPRTQKPIVFVGKTVTFDSGG
                     ISLKPSENMEQMKADMTGGAEVLATIRAASRLRLPINVIGLLPVTENMPGGRATKPGD
                     ILNMLSGKTVEVQNTDAEGRLILADGLAYATRLKPACVIDIATLTGAAIVALGQFAIG
                     MLGNDATLKADLRKAGDHTGERVWEMPLWDEYFEQLKSDVADMRNIGGRGAGMITAGM
                     FLSKFVGNHPWVHLDIASTDWSSGERPYIPKGPTGTGT"
BASE COUNT          655 a          685 c          797 g          637 t
ORIGIN      
        1 agtatgcgat acgacagatg ccgccgttgt cgctgggccc ccttcgacgg aagaagctgt
       61 atctcatggg acatgacgtt tccctcattg agcaggttgc gagggagtat gttgagttat
      121 tcgtcattcc acgtgtctca acccgggcct tgagccgctt ggtccgtcat caggaagcag
      181 gacatgttgt cgcattcgtc agcggatcac ctgaatgttt ggtcagaccc ttagcggcca
      241 aacttggcgt ctcgctggtc tttggcgcaa gattggaaac acaggcagga atgtacacag
      301 gaaacgtctt tgccccgcta ccctatggcg aagggaaagg ccgacatgtg gagcgtctcg
      361 ccgcccggta taatctggat ctgagtcgca gctatgctta tggggatagc cccggtgatt
      421 ttcacgccct tcaactggtt gggcatccct ttgtggtgaa tcccattcgc ggcatggccc
      481 gtatcgccca ccggcaggga tggcccatta cccaatgggc ttaacgatac tgctctcctc
      541 ccggtcacca ccacttgtca gtcccatatg acccgacgtc tgaagagagt tggaaacctg
      601 tatggctaca ctgcctttac tgaacatcac agaaatcttt catagcattc aaggagaatc
      661 gactcgtgtg gggcaaccat gcgtgtttgt ccggttaacg gggtgtcctc tccgttgtac
      721 ctggtgcgat acggcatatg cattttatgg tggaaccagc atgccaatgg aggccatttt
      781 agcacaggtg cgatcttatg gttgctctct cgtcgaggtg accggcggag aaccgttgca
      841 tcaacccggc tctctggtat tgttaacgga actgtgcgag gcaggatttc aggtgatgct
      901 ggaaaccagc ggggcatgtg atatttctca agtggatacg cgtgtttcga ttattctgga
      961 tgtgaaatgc ccggggagcg ggatgacgga ccgtatgcgg tgggataccc ttacgcatct
     1021 gtcccgaaaa gacgaggtga aatttgtctt gaaggaccgg gccgattacg aatgggcgcg
     1081 agatattatc cagcaatacg ccttggggag ccggtgtcct gtgcatttga gccctgtatt
     1141 tggtgcactc cacctacaat ccttagcaga atggattctc gaggatcgac tacccgtccg
     1201 ctttcaactc caattgcata aagtgatctg ggatcctcag acccgcggcg tgtaatgggc
     1261 cgggaaggaa tcgtatacag tatgggcgac tttatttatt gaaggaggca tatgaatatt
     1321 caagtaaaaa gtggtgaagc caccactgcg gctacggata tgttggtgtg tctggagtat
     1381 gaacaggaaa aaaactggag caaagctctc aggccggtcg atcggaaact cgggggacag
     1441 ttgagtgact tgcgaaaaag tggagaattt tccggaaagc cgaataatat ggcattgctg
     1501 catatcgacg ggaaactgtc cgcaaaacgc gtgcttctcg tcggacttgg tccacgtgaa
     1561 acggtgacat tagaacgaat tcgccaggca atgggtaccg ccgccaaacg cgctcgaagt
     1621 gtgaaagcca aagggatcgt cagtgtgatg ccggatgtgc caaaggccgc tggccacatg
     1681 actgatgtgg cccaagcgat ggtggaagga ttgattcttg gggattatcg gtttaatgag
     1741 taccgcactg atcgttcgga cgataaccac accctgcaat cctgcacggt actcgctccc
     1801 tccagctcca gcatgtctga agttaaagag ggaactaacc gtggaaagat tctgggtgac
     1861 gcaacctgct tcgtccggga tctgtgtaac catcccgcca acgtgatgac gccttcccgc
     1921 gtggttaccg aagcccgaaa aattgccagg gaggccaaaa tccggctcaa ggtgtttgac
     1981 cgtcggcagc aggaaaaact cggcatgggg ggattgttgg gagtttcaag aggcagcatc
     2041 gaaccgcccc aattcattat ccttgaatat gcgggtggcc cacgcacgca aaagcccatc
     2101 gtgtttgtgg gcaagactgt gacgtttgac agtggtggta tctccttgaa accttccgaa
     2161 aacatggagc agatgaaggc agatatgact ggcggagccg aagttttggc aacaatccgg
     2221 gctgcctccc gtctccggct tccgataaat gtcatcgggc tcttgcctgt gacagaaaat
     2281 atgccgggag gacgggcaac gaaacctggc gatattctga acatgttgtc cggcaaaacg
     2341 gtagaagtcc aaaataccga tgccgaaggc cgattaattc tggctgacgg gttggcctat
     2401 gccacacgcc tcaaacccgc ctgcgtaatt gatattgcga cgctgaccgg cgctgccatc
     2461 gtcgccttgg gacaatttgc cattgggatg ttgggcaatg atgctacctt aaaagccgac
     2521 cttcgaaagg ccggcgatca taccggagaa cgcgtatggg aaatgccgct ttgggacgaa
     2581 tactttgagc aattgaagag cgacgtggcc gatatgcgga acattggcgg acggggagcc
     2641 ggcatgatta ccgccgggat gtttttatct aaattcgtcg ggaatcatcc gtgggtccat
     2701 ctggacattg ccagcaccga ctggagtagc ggagaacgcc cttatatacc caaaggcccg
     2761 actggtacgg gcac
//
LOCUS       DATIAL010000080        10063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_336316, whole genome shotgun sequence.
ACCESSION   DATIAL010000080 DATIAL010000000
VERSION     DATIAL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10063
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336316"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VLA60_02610"
     CDS             <1..280
                     /locus_tag="VLA60_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08274.1"
                     /translation="AVARWIQSEHEAGHKRREESPTEEEIPKILPDVLRRLHAEQAEK
                     LAEQAWINPVGEKQYLISDDELRSFYGLTIGQISHSHWWSVQKIEHEF"
     gene            320..2236
                     /locus_tag="VLA60_02615"
     CDS             320..2236
                     /locus_tag="VLA60_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08275.1"
                     /translation="MDRIKVACLLRIADALHLDQLRAPPFLRTLIRPEGLSALHWSFQ
                     EKLARPYIESDEVVFTTGHPFDRDDAEAWWLAYDTLSLIDRELRDVDLLLRMKRREVL
                     KVSRVKGIGSPEALAKTVQTRGWRPVDARIKVSDVPRIVESLGGSKLYGDDPFVPIRE
                     LIQNSADAIQARRKFQKRSENWGEIFISIRELNGGNWLIVEDNGIGMSESVLTGPLLD
                     FGNSFWRSSLVMEEFPGLVSSGMNAIGRFGIGFFSVFMLGDIVRVTSRRCDRGEETAS
                     VLEIRGGLAGRPILWKAKPGEAPLDGGTRVEVLLKNAPNSPNGLLWYSNPFQKRNLSL
                     DKIVGSLAPALGVSLASEGGDGSEKKLVSKAEDWLSVTDSELLERLCLFGNVDKSEIS
                     AVSLLRPLVDDKGTVYGRASITPRKYSFWNEGSGCVTVSGLRANLLTNVQGVLMGEPL
                     TAVRDLALPLVPKEVLATWATEQGNLLLERRVYDEVKAKAAETILECGGDIGDLPIVR
                     WGGAWLNTDDFSVKIRECKELFINFEGEFSYDEEVDSMHPRDFKDGFEEDDSIIIVPK
                     HDGNIIVDRRTKWPACLYDERSGTNRLTEHVKKLIQTEWADQFEEYEEFCLVGEANNQ
                     EVMREISVFRRADV"
     gene            complement(2509..2811)
                     /locus_tag="VLA60_02620"
     CDS             complement(2509..2811)
                     /locus_tag="VLA60_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08276.1"
                     /translation="MSEGAQIGKPHPTAGELTKVFVTPKHYQQKTPDLIKRLQDHVLQ
                     EPCKIAVSLEIEVVPSLPKIRSDKNMSPKGQGTRSRSRRHFHPGGINSDHSVFILC"
     gene            3119..3529
                     /locus_tag="VLA60_02625"
     CDS             3119..3529
                     /locus_tag="VLA60_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08277.1"
                     /translation="MVWNGFPSEDGEPCRWNLGQTASHLVDHNAILYWPRAGTFNMPY
                     GVTATGSWLIAADTANSRLIGWHDSNLQTGAKAQASAGQPTFQFKGDNQWSFPVRGSL
                     CWPYNVQVCGKFVVVSDSGNNRVHLWDIDGALLS"
     gene            3526..5943
                     /gene="hypF"
                     /locus_tag="VLA60_02630"
     CDS             3526..5943
                     /gene="hypF"
                     /locus_tag="VLA60_02630"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HSF08278.1"
                     /translation="MMETVKVRSSNDQAFNIQVFGLVQGVGFRPRVWQLAQRFDLRGR
                     VSNNGKGVQILVAGEEDNLRHFIHELTHNPPPLAKIAEIFTSGISLSDVPEDTFVIAG
                     TAKSPVQTGIVPDAAPCPECVKEIFDPFARRYRYPFTNCTHCGPRLTIQEGIPYDRPS
                     TTLKNFPLCEACLKEYHDPQDRRFHAQPIACHACGPKVWIERMDGKPLTVHSYTMLDE
                     ADAVCSLLRKGHILAIKGLGGFQLACDATRENAVSRLRALKHREGKPLALMARDLEVI
                     RRYCAVGEREAELLQSQAAPIVILKRHPERALAPSVAPGMSTYGCMLANSPLHHLILH
                     RMAHPIVLTSGNHAGEPQWIDNDQAKGHLKNIAEFVVLHNRGIVQRVDDSVVKVMDQV
                     PRVLRRSRGFAPIPIWLPSGFEHSPAILAMGGELKNTFCLLKDGQALLSHHIGDLEDS
                     LTYADYQRALSHYQHVFEHKPDRIAVDLHPEYLSSKFGRAQANLRHLPLVDIQHHHAH
                     LAACLVENGVPLTTEPVLGVILDGLGYGEDDTMWGGEFLLADYGGFTRLGSFKPVPML
                     GGTQAIKEPWRNTYAHVMAEMGWAEFAMNYSDLPLFDFLEKKPRMMLDRMLSHRINSP
                     LASSCGRLFDAAAAAMGICTEHVLYEGQAAMEMEALVDDKTLHHEDELLAYPFSIPRL
                     KHSGLPYVEPLGMWQALLGDLILHTPVPVMAARFHKGLANVICNMVTQCSQFPDGHQT
                     VVLSGGVFQNQVLFELVKRRLAAKGFTVLSHKQVPMNDGGIALGQAAIAAARSLQETN
                     EVRSTCV"
     gene            5934..6215
                     /locus_tag="VLA60_02635"
     CDS             5934..6215
                     /locus_tag="VLA60_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HSF08279.1"
                     /translation="MCLGIPGQIVEISNVGHKLAIVNVGGVRREVNIACIVDEEHSPG
                     SCVGDWVLIHVGFAMSRIDEKEAKRTIELLTEMGEVQTEIQAMRQSNTS"
     gene            6227..6919
                     /locus_tag="VLA60_02640"
     CDS             6227..6919
                     /locus_tag="VLA60_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HSF08280.1"
                     /translation="MSNHKTLASLYPFLQPSSKDSAHAKSILLESVRMKSQDSTQVIQ
                     TFFDTQAEKVVAAARAVADIYQHEGRLFTMGNGGSSCDAAHIAVEFLHPVTTGRPALP
                     AVNLAADQAMMTAVGNDVGFDHIFVRQLIAQGKAGDGVIGVSASGNSENIMRTFRKAK
                     EMGVTTIGLTGGLRGAMATSSAIDHCLVVPTESIHRIQECHVLIYHILWDLVHTLLAD
                     HRGASATNEETL"
     gene            6916..8061
                     /gene="hypD"
                     /locus_tag="VLA60_02645"
     CDS             6916..8061
                     /gene="hypD"
                     /locus_tag="VLA60_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HSF08281.1"
                     /translation="MKFVDEFRDPQKARILLQEIEGLVKRIPICRDRPIWIMEVCGGH
                     THTIFRYGLNKMLPKEVEFIHGPGCPVCVLPRGRVDDCIALAERDEVIMTTFGDAMRV
                     TGSKKNLLQAKAEGADVCMVYSPLDALNLARVNPDREVVFFGLGFETTMPCTALTILQ
                     AKQEGIKNFSIFCNHITIIPTIKAILDSPDLQLDGFLGPGHVSMVIGTKPYSFIAEYY
                     RKPITIAGFEPLDILQSVWMVLKQLAERRCEVENQYTRIVPPDGNTQSLEAIQKVFEL
                     REFFEWRGLGSIDHSGVRVREEFSAFDAERKFPLPNLKIADPTSCQCGEVLKGVIKPS
                     QCKVFGTACTPQNPLGALMVSTEGACAAYYNYGWIPEEDLHPARSPV"
     gene            8094..9191
                     /gene="hypE"
                     /locus_tag="VLA60_02650"
     CDS             8094..9191
                     /gene="hypE"
                     /locus_tag="VLA60_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HSF08282.1"
                     /translation="MINPESLTSPQPAFVKTKPMLNRATNDERITLAHGSGGKAMRDL
                     IQNLLVPTFNNPILDNLEDQATIPLGQLFQHGDRLAFTTDSYVVDPLFFPGGNIGELA
                     VNGTVNDLAVSGARPLFLSCGLVIEEGFPLDTLALIASAMKQAADRAEVSIVTGDTKV
                     VEKGSADKLFINTAGIGVIREGVAMAATKAQAGDAIITNGFIGDHGVAILLARNELAL
                     EADVQSDTQSVHDLISHMLDVCSNIHCLRDATRGGLATVLNEFASASNVSIQINEPQV
                     PIRESVRGACEILGLDPLYLANEGKIVAVVPYENAHELIEAMRDHPLGKHSAIIGRVN
                     ASPAGIVILATTFGGTRIVDTLVGDQLPRIC"
     gene            9204..9770
                     /locus_tag="VLA60_02655"
     CDS             9204..9770
                     /locus_tag="VLA60_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08283.1"
                     /translation="MKLLTSEILEILEDGQLQRLTYAGLLAFHQDNAVWGASVGYRAM
                     QAAGWALSHEQLWDRKGLFVVSARPGPGVRDAIEFVTRCVSRKRFQLLDPHQPAVCNQ
                     HMQFRWWVTHHDRTVDVELKKGFVPAQFFELLERVGSDRETPSDSLNLEKLKADLTER
                     LWHEPLTALFDVTASGKSTVLDRFACTN"
     gene            9758..>10063
                     /locus_tag="VLA60_02660"
     CDS             9758..>10063
                     /locus_tag="VLA60_02660"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HSF08284.1"
                     /translation="MHELGITRNIVSIVTEKAGGEPVRRVTIAIGKLSAVLPEAIRFC
                     FDIVSQGTVVEGADLEILEIPGTAECRTCGTHFELEQLYGRCPCGSVDITRLTGEELL
                     "
BASE COUNT         2445 a         2321 c         2727 g         2570 t
ORIGIN      
        1 tgcggttgct cgatggattc aatcagagca tgaagctggg cacaagagga gggaggagag
       61 tcctacggaa gaagagatcc caaaaattct ccctgatgtg cttcgacgac tgcatgctga
      121 acaggcagaa aaattagccg agcaggcttg gattaaccct gttggtgaaa aacagtattt
      181 gattagtgat gacgaattac ggtcatttta tggattaact atagggcaaa tatctcacag
      241 tcattggtgg agtgttcaga aaattgagca tgagttttga gaaagtttag ggggttgtcc
      301 ccaaagaacc cgtaatagtg tggatcgcat taaggtagcc tgccttctgc ggatcgccga
      361 tgcattacac cttgaccagc ttcgtgcccc gccattcttg cgaacattaa tcaggccaga
      421 aggcctatca gcactgcact ggtcttttca agagaaattg gctcgcccgt acattgaatc
      481 agatgaagtt gtttttacga ctggtcaccc ctttgatcgc gatgacgctg aggcttggtg
      541 gttggcttat gataccctgt ccttgatcga tcgtgagctt cgagatgttg atttattgct
      601 aagaatgaaa agacgggaag ttttgaaagt aagccgagtg aagggaatcg ggtctcctga
      661 ggctttagca aaaacagttc aaacccgggg ttggcggcct gtcgatgctc gaatcaaggt
      721 ttctgatgtt ccacgtatag tcgagagtct cggcggatca aaactatatg gagatgatcc
      781 attcgttcct ataagggaac ttattcaaaa ttcagcggat gccattcaag cacgtagaaa
      841 atttcaaaag cgatccgaaa attggggaga aatttttatc tcaattagag aacttaatgg
      901 tgggaattgg cttattgtgg aggacaatgg aattggaatg tccgagtctg tcctgacagg
      961 gccattgttg gattttggaa attcattttg gcgatcttcc cttgttatgg aagaatttcc
     1021 cggactggta tcgtctggaa tgaatgccat cgggcggttc gggattgggt tcttttcggt
     1081 tttcatgctt ggtgacattg tccgagtgac aagccgtcgt tgtgacagag gcgaagaaac
     1141 agcttccgtg ttagaaatta gaggagggct agctggtcgg ccgattttat ggaaggcaaa
     1201 acccggcgag gctccccttg atggtggtac acgggttgag gttctcttga aaaatgcccc
     1261 taattctccc aacggattac tttggtatag caatccattt caaaaacgga acttatcatt
     1321 ggataaaatc gttggatcct tggcacctgc tcttggggtc agtctggcat ccgaaggtgg
     1381 agacggttca gagaaaaaat tggtttccaa ggccgaggac tggttaagcg taactgattc
     1441 agaattattg gaaagattat gtctttttgg aaatgtcgat aaatcagaaa tctcagcagt
     1501 ctctcttttg cgacctttgg tcgatgacaa aggcaccgtc tatggacgtg cctcaatcac
     1561 tcccaggaag tattctttct ggaatgaagg ttctggctgt gttacggttt ccggcttgag
     1621 agcaaacctc cttacaaatg tacaaggggt tcttatgggt gagcctctca cagcagtacg
     1681 ggacctggct cttcctctgg taccgaaaga ggttttagcg acttgggcaa ctgagcaagg
     1741 aaaccttctt cttgaaaggc gggtctatga cgaggttaag gccaaagccg cagaaactat
     1801 cttagagtgt ggaggtgata taggggatct accgatagtc cgttggggag gggcatggct
     1861 taacactgac gatttttcag ttaaaatcag ggagtgtaaa gaattgttta tcaattttga
     1921 gggggaattt tcttatgatg aggaagtgga ttcaatgcac cctcgggact tcaaagatgg
     1981 tttcgaagaa gatgacagta ttattatcgt tccaaaacac gacgggaata tcattgtgga
     2041 tcgtagaaca aaatggcctg catgtttgta tgatgagaga agcggtacaa acagacttac
     2101 agaacacgtc aaaaaattaa ttcaaactga atgggcggat caatttgaag agtatgagga
     2161 attttgtcta gttggtgagg caaataatca ggaggttatg cgagaaatat ccgttttcag
     2221 gcgagccgac gtgtaatcca tctaaaatta atgctgtaac ttttatctac gaactattct
     2281 ctgctgtgtt aaaagcctaa cttttctggt aaaagaaatt ggaaaaactt taagcgattc
     2341 tttaacactt ccagtgcatg tcaggagaat gagtttttct aaagtacctt agttatttat
     2401 ttctcagcgt atcttcctca taatttccat gaaatttgtc ggcggaattt tttgataaaa
     2461 gattgagcgg cccgctgatg aagagtcata gggctatatt ggaaggattc agcaaagaat
     2521 aaatactgag tggtcggaat ttattcctcc agggtggaaa tgtctcctgg atcttgaccg
     2581 agttccttgg cctttaggac tcatgttttt gtcggagcga attttgggaa gtgaggggac
     2641 cacctcaatt tcgagtgaga cagcgatctt gcacggttcc tgtaagacat gatcctgcaa
     2701 acgtttgatg agatcgggtg ttttctgctg gtagtgcttc ggagttacga agactttggt
     2761 cagttctccc gcagtcggat gtggtttgcc gatttgggcg ccttccgaca cacctttacc
     2821 ccctcggaat tcgattactg gtaattctca tctggggttt ttccgatggc aagaaattgt
     2881 gacgatttac tgaaaaggtt ttttacgatg cgagctttgt tggccgctga agggatgatg
     2941 attcctacat tgattactcc caacgtagtt ttcactttgg aatatttgtt gacatccata
     3001 actggccgtc aaataataaa aaaaacgagc gattcagcat gaatcaggaa gccttatcgg
     3061 gcctttccga tcatcgcggt aatgcaggtc tgaatgcaat gcggggaata accggatcat
     3121 ggtctggaat ggatttcctt cagaagatgg agagccatgt cgttggaatc tcggccaaac
     3181 tgcgtctcat ctggtcgacc ataatgccat cctgtattgg ccccgggccg gaacattcaa
     3241 catgccatac ggggtcacgg ccaccggttc ctggttaatc gcggcagata ctgcaaattc
     3301 ccgattaatt ggttggcatg attccaacct acagacagga gccaaggcgc aggcgtcggc
     3361 aggccaaccc acttttcagt ttaaagggga taatcaatgg agttttcccg tgagggggag
     3421 tctgtgctgg ccctataatg ttcaggtgtg tggcaaattt gtagtcgtgt cggactcagg
     3481 caacaaccgt gttcatctgt gggacattga cggagccttg ctttcatgat ggaaacagta
     3541 aaggtaagaa gctccaacga ccaggcattc aatatccaag tgtttggatt ggtccagggt
     3601 gtcggttttc gtccgagggt ctggcaactg gctcaacgct ttgatcttag aggtcgtgtt
     3661 tccaacaatg gaaagggtgt tcaaattctg gtcgcaggcg aggaagataa cctcaggcat
     3721 ttcattcatg aactgactca caatcctcca ccactcgcta agattgccga aatcttcaca
     3781 agcggcattt ccctgtctga cgtcccggaa gacacgtttg tcattgccgg taccgccaaa
     3841 agtcctgtgc agaccggcat tgttccggat gcggcccctt gtccggagtg cgtgaaggaa
     3901 atatttgatc cttttgcacg gcgctaccgc tatcccttta ccaactgcac gcattgcgga
     3961 ccccgcctga cgattcagga agggattccc tacgaccggc cttctaccac actgaagaat
     4021 tttcctctgt gcgaggcctg cctgaaggaa tatcacgatc cccaagaccg gcggtttcat
     4081 gcccaaccga tcgcgtgcca tgcatgcgga ccgaaagtgt ggattgaacg tatggatgga
     4141 aaacccttga cggtgcattc ttatacgatg ctggatgagg cagatgcggt ctgcagtctc
     4201 cttcggaaag ggcacattct ggccattaaa ggattaggcg gttttcaact tgcctgcgat
     4261 gccacccggg agaacgcggt cagccggctt cgtgccttga aacacaggga gggaaaacct
     4321 ctggcattaa tggcgcgcga tctggaagtg attcgccggt attgtgcggt aggggagagg
     4381 gaagccgaac tcctacaaag ccaggcggcc cccattgtca tccttaagcg ccatccggag
     4441 agggcccttg cgccaagtgt tgctcccggc atgtccacct atggatgtat gttagccaac
     4501 tcaccgctgc accatttgat cctccatcgc atggctcacc cgattgtgtt gaccagcggg
     4561 aatcatgccg gcgaacccca gtggatcgac aatgaccagg ccaaaggaca tttgaaaaac
     4621 attgccgagt ttgtggtcct gcataatcgg gggattgtcc aacgtgttga cgattcggtg
     4681 gtcaaagtca tggatcaggt tccacgagtg ctgcgacgga gtcgagggtt tgcgcccata
     4741 cccatatggc ttccctcggg cttcgagcac agtcctgcta ttcttgctat gggcggtgaa
     4801 ctcaaaaata ccttctgctt actcaaagat ggacaagccc tgctctccca tcacattgga
     4861 gacttggaag actctttgac ctatgccgat tatcaacgtg ccctctccca ttaccaacac
     4921 gtgtttgagc acaagcctga ccgcatcgcc gttgatctgc acccggaata cctgtcgagc
     4981 aaattcggac gggcccaagc caacttacgc catctgccgc ttgtcgatat ccaacatcac
     5041 catgctcatc tcgctgcctg tctggtcgaa aacggggttc ccttaacgac tgaaccggtc
     5101 cttggggtga tcttggatgg gttgggctat ggggaagacg atacgatgtg gggcggagag
     5161 tttttgctgg cggattatgg gggctttact cgtcttgggt cctttaagcc cgtaccgatg
     5221 ctcggcggaa ctcaggcgat aaaggaaccg tggcgaaaca cctatgcgca tgtgatggca
     5281 gaaatgggat gggcggagtt tgcgatgaac tactccgatc tgccattgtt tgattttctc
     5341 gagaaaaaac ctcgcatgat gctggatcgc atgctgagcc atcggattaa tagcccgctt
     5401 gcgagttcct gcggtcggtt gttcgatgcc gccgccgctg cgatgggtat ttgcacagag
     5461 cacgtgttat atgaggggca agctgcaatg gagatggaag cgctggtgga tgacaaaaca
     5521 cttcatcatg aggacgaatt gttggcgtat ccattttcta ttccgcgcct gaagcactct
     5581 ggattaccct atgtcgaacc gttaggaatg tggcaagcgc ttttgggcga ccttatcctc
     5641 cacacaccgg ttccggtcat ggccgcacgg tttcataagg ggcttgccaa cgtgatttgt
     5701 aacatggtca ctcagtgctc acaatttcct gacgggcatc aaacggtggt cctttcaggt
     5761 ggcgtgtttc aaaatcaggt gttgtttgaa ctggtgaaac gtaggttggc ggcaaaagga
     5821 ttcaccgtcc tttcccacaa acaggttcca atgaacgatg gtggcattgc ccttggacag
     5881 gccgcgattg ctgcagctcg ttccttgcaa gagaccaatg aggttcgatc cacatgtgtt
     5941 taggtatacc cgggcaaatt gtagaaatca gcaatgtggg acataagctc gcgatcgtga
     6001 acgtgggtgg ggtgcgacgg gaagtgaata tcgcctgcat tgtggatgag gagcattccc
     6061 ctgggtcatg tgtcggggat tgggtgctga ttcacgtggg atttgccatg agtcggattg
     6121 acgaaaagga agctaagcgg acaatagagt tgctgactga aatgggagaa gtgcaaacag
     6181 aaatccaggc gatgcgtcag tcaaatacct cctaaaggat gaccgcatgt ctaatcacaa
     6241 aacattagcc tccctctacc ccttcctgca gccgtcttcg aaagattcgg cacatgcaaa
     6301 atccatattg cttgaatcgg tgcgaatgaa atcgcaagat agcacgcagg tcatccaaac
     6361 attttttgac acacaggccg aaaaagtcgt ggccgctgcc agggccgtgg cggacattta
     6421 tcaacatgag ggacggctgt ttacgatggg caacggcgga tcgagctgtg atgccgccca
     6481 tattgccgtc gaatttttac atcccgtgac caccgggcgc cctgcgcttc ccgccgtcaa
     6541 tcttgcggca gatcaggcca tgatgaccgc tgtaggaaat gatgtggggt ttgatcacat
     6601 ttttgtgcgc cagctgatcg ctcagggaaa ggccggggac ggggtgatcg gcgtgtccgc
     6661 gagcggtaat tccgaaaata ttatgcggac ctttaggaaa gctaaggaga tgggggtgac
     6721 caccattggc ctcaccgggg gacttagagg agccatggca acctcttctg ccattgatca
     6781 ttgcctggtt gtgcctactg agagtattca ccggatccag gaatgtcacg tcctgatcta
     6841 tcacattctt tgggatctcg tgcacaccct cctggctgat catcggggcg cgtcggccac
     6901 aaatgaggag acgctatgaa atttgtcgat gaatttcggg atccccaaaa agcccggatc
     6961 cttctccagg aaattgaggg actggtgaaa cggataccta tctgccgcga tcggcccatt
     7021 tggatcatgg aggtctgcgg tgggcatacc cacacgattt ttcgctatgg cctcaataaa
     7081 atgctcccga aggaagtgga gttcattcac ggaccgggct gtccggtttg cgttctgcca
     7141 cgaggccgcg tggatgactg tattgctctg gctgaacggg acgaggtgat catgaccaca
     7201 ttcggggatg ccatgcgggt gaccggctcc aagaaaaatc tcctacaggc caaagccgag
     7261 ggagccgatg tgtgcatggt ctattcacca ttggacgccc tgaatcttgc gcgagtgaat
     7321 cctgacagag aagtggtgtt tttcgggctt ggttttgaga ccaccatgcc ctgtacggcg
     7381 ttgaccatac tccaggccaa gcaagaaggg atcaaaaatt tctcgatctt ttgtaaccac
     7441 atcacgatca ttcccacgat caaggccatc ctggattctc ctgatcttca gcttgacggc
     7501 tttttgggcc cgggccatgt gagcatggtg atcggaacga agccctactc atttatcgct
     7561 gaatattatc gaaagcccat caccatcgcc gggtttgaac cactggacat tttacaatcc
     7621 gtgtggatgg ttctgaaaca attggcggag agacgctgtg aagtcgagaa tcaatataca
     7681 cggattgttc ctcctgatgg gaacacgcaa tcgttagagg ctattcaaaa agtgtttgaa
     7741 ctgcgggagt tttttgaatg gcgggggttg gggtctatcg atcactccgg ggtccgagtc
     7801 cgtgaggaat tttctgcgtt tgacgctgaa cgaaaatttc ctcttcccaa tctgaaaatt
     7861 gccgatccca cgtcctgcca atgcggggaa gtgttgaagg gggtgattaa gccgagtcaa
     7921 tgtaaggtat ttggcacagc ctgcactcct caaaatccct tgggagcgtt gatggtttcg
     7981 acggaaggcg cctgtgccgc ctactacaac tacggctgga ttcctgaaga agatctgcat
     8041 ccggcaagaa gcccggtgta agtcataaaa aaaatgtaaa gaatcggttg aatatgataa
     8101 atcctgaatc gcttacttct ccacaaccgg ctttcgtcaa gaccaagccc atgctgaacc
     8161 gagccactaa cgatgaacgg attaccctgg ctcatggaag tggcggcaaa gccatgcgag
     8221 atctcatcca gaatctgttg gtcccaacct ttaataatcc cattctcgat aacttagaag
     8281 atcaggccac gattccgttg gggcaattgt tccaacatgg tgaccggctg gcatttacga
     8341 cggatagcta tgtcgtggat ccgttgtttt ttccgggagg caacattggc gagttggccg
     8401 tgaacgggac ggtcaatgac ctggctgtca gcggggcacg cccgctattt ttatcgtgcg
     8461 ggttggtgat tgaagagggt tttcccttgg acacgctcgc cctcattgcc tcggctatga
     8521 aacaggccgc tgatcgagca gaggtctcta ttgttacggg agataccaaa gtcgttgaaa
     8581 aaggttccgc ggataagctc ttcattaaca cggcgggtat cggtgtgatt cgagaagggg
     8641 ttgccatggc agcgaccaag gctcaggctg gggatgccat cattacgaat ggttttatcg
     8701 gtgaccacgg cgtggccatt cttctggctc gaaacgaatt ggccctggaa gccgatgtac
     8761 aaagcgacac ccagtcagtc cacgatttga tttcccatat gctggatgtc tgctccaata
     8821 tccattgttt gcgggatgcc acgcgcggcg gattggccac cgtccttaat gaattcgcct
     8881 cggcctcgaa cgtgtccatt cagatcaacg agccacaggt gcctattcga gaatccgttc
     8941 gaggagcctg tgaaattctc ggtcttgatc ccctgtactt ggccaatgag ggaaaaattg
     9001 tggcggttgt accttatgaa aacgctcacg aacttattga ggccatgcgg gaccatcccc
     9061 taggcaaaca cagcgccatc atcgggaggg tcaacgcctc accggcggga atcgtgatac
     9121 ttgccactac cttcggaggt acacgaattg tggatacgct ggtgggggat caattgccgc
     9181 gaatctgcta gagaagaata gtcatgaaat tattgacttc tgaaatactg gaaatccttg
     9241 aagacgggca actccaacgg ttgacctatg cggggcttct tgcgtttcat caggacaatg
     9301 cggtgtgggg agccagcgtc gggtatcgcg ccatgcaagc ggcgggttgg gctctgtctc
     9361 atgagcaatt atgggatcga aagggtttat tcgttgtcag cgcccgtccg ggtcctggtg
     9421 tccgtgatgc cattgagttt gtgacacggt gtgtgagtcg aaagcggttt caattactcg
     9481 atcctcacca acctgctgta tgtaatcagc acatgcaatt tcgttggtgg gtcacccatc
     9541 acgatcgaac ggttgacgtg gaactcaaaa agggatttgt ccctgctcaa tttttcgaac
     9601 tcctggagcg agtggggtcg gaccgggaaa ccccttcaga ctctctcaat ctcgaaaagc
     9661 tgaaagccga tctcaccgag cggctgtggc atgaaccctt aacggctctt tttgacgtca
     9721 ccgccagtgg aaaatccact gttctggacc ggttcgcatg cacgaattag ggatcactcg
     9781 aaatattgtg tccattgtca cagaaaaggc cggtggagaa ccggtaaggc gcgtgacgat
     9841 cgccatcggt aaactcagtg cagtattgcc cgaagccatc cggttttgtt tcgacattgt
     9901 cagtcaagga accgtggtgg aaggcgctga tttggagatc cttgaaattc cgggaacagc
     9961 tgaatgccga acctgtggaa cgcattttga actcgagcaa ctctacggcc gatgtccgtg
    10021 tggttccgtc gacatcacgc ggctcacggg agaggaactc ctc
//
LOCUS       DATIAL010000081         4344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1304160, whole genome shotgun sequence.
ACCESSION   DATIAL010000081 DATIAL010000000
VERSION     DATIAL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304160"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..714)
                     /locus_tag="VLA60_02665"
     CDS             complement(241..714)
                     /locus_tag="VLA60_02665"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HSF08285.1"
                     /translation="MNPEPDKKGAAVKVPPPLIVLSVLLLAYALHYVIPFRIAHSSVL
                     VFAGFALVGISLLIIGITAWSFRRLKTHIEPWKPTTTIITTGCFAVSRNPIYLALCVA
                     CLGIGMILHSWWVVFSVIPAALLLYHFVIAREEAYLEKKFGEAYVQYKARVRRWL"
     gene            complement(711..908)
                     /locus_tag="VLA60_02670"
     CDS             complement(711..908)
                     /locus_tag="VLA60_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08286.1"
                     /translation="MYRPHALCMCALLLTGCSSNIKDTDVLSGKSGILPPATAKTVFI
                     QNRNSSDNHAVTLSDLGSRLG"
     gene            complement(1124..2071)
                     /gene="corA"
                     /locus_tag="VLA60_02675"
     CDS             complement(1124..2071)
                     /gene="corA"
                     /locus_tag="VLA60_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSF08287.1"
                     /translation="MINAYALHDGKFQALPVPELSNSLDVAMWIDLTAPTDDERQIIE
                     TLYEQPLPTSKEVREIEASARVNEGEDSLHIYSFFFQEEDHRATTRAVAFTIKDNCII
                     TLHDHPIPLFRLMRRRARHESDLIRDPLSVLLALFELKIEQLADQLEATHLALETISQ
                     TVLAKEGRDLQGAIDLLTREEDGNGKVRLCLMDTQRALAFLLRRGRGDETVRDWIGEI
                     LRDIDSLLPHNAFLFEKVNFLMNAAMGLINIEQNQIIKIFSIAAVVFLPPTLIASIYG
                     MNFDVMPELHWWWGYPLAVCAMVLSGIAPYWYFKRKGWL"
     gene            2168..2389
                     /locus_tag="VLA60_02680"
     CDS             2168..2389
                     /locus_tag="VLA60_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08288.1"
                     /translation="MIAGVTASSEPHPDQKTITKRPVLQQRDSMKQAMHPWVTVLCYT
                     AQTGRFSPGEEEPRERKNANIFNSGKNQE"
     gene            complement(2518..4047)
                     /locus_tag="VLA60_02685"
     CDS             complement(2518..4047)
                     /locus_tag="VLA60_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08289.1"
                     /translation="MRPRRVTILDLVTKGPTNSLYARVMNQNLASIMPQVVGVWCEEL
                     GHQVRFVCYTGREDLQQELLDETDIVFIGAFTRSALTAYAISQMFRARGAVTVLGGPH
                     ARSYPQDAAKYFDYVVGFTDKTLIQDILADCAQHRPLGVQLAAARQPTALPGAKERWK
                     FIEPTIAKAPTSFKVVPMIGSMGCPYTCGFCVDANVDYQPLSFDQISEDLKFLRTKLK
                     RPIVAWHDPNFGVRFQEYMNAIETAVPPNSIDFIAESTLSLLAEPHLKRLKQNGFKAI
                     LPGIESWYDMGDKSRTGRHRGQEKVKQVADHINLILRYIPYVQVNFVLGLDSDQGPEP
                     FDLTKQFLDLAPGAFPAFSLLTAFGQAAPFNRELQRDGRVLPFPFHFLDNNHAMNVRP
                     KHYAWPEFYDHVVGLSQHAFSWPMIAKRMMLNRGWIPKWFNFIRSVSSEGFGRIKYHT
                     KIRGLLDTDVSVRNFLEGETLELPKFYARKIRGKLGPLYDLLPEGATMHDHHAYLNSY
                     Q"
BASE COUNT         1088 a         1161 c         1122 g          973 t
ORIGIN      
        1 aaattcccct cctttgtaag gaggggcata ggggaggtag agataccaaa gagaaaacgt
       61 taggaggcgc cgtttcccgg ttaaaaacca atattggagt ttctggggtc aggattagag
      121 gaggatatag tcaggagaca ggtatgggtg acaaaagcga aggatgcatc cgcgttaaca
      181 gatacccgcg ataccgacgt gcaaagtttt ggcaaccgct atcgtggtag aactctgcgc
      241 tcacagccag cgccggacgc gcgctttata ctgcacatag gcctcaccaa actttttctc
      301 gagatacgcc tcttcgcgtg caatgacgaa gtgatagagc aaaagggctg cagggatgac
      361 gctgaatacc acccaccaac tatgaagaat catgccgata ccgagacacg ccacacacaa
      421 cgctagataa atcggattgc gtgagacggc aaagcatccg gtcgtgatga tcgtggtggt
      481 tggcttccat ggttcgatat gagttttcag ccgtcggaat gaccatgcgg tgatgccgat
      541 gatcaagagt gagatcccga ccagtgcaaa accggcaaac actaaaaccg atgaatgtgc
      601 aatcctgaag ggaatcacgt agtgaagagc ataggccaga agcaggacgc tgagaacaat
      661 gagtggcggc ggcaccttga ccgccgcacc ttttttgtca ggctctggat tcatcccagg
      721 cgagagccga ggtcgctcag ggtgacggcg tgattgtcgg atgaattgcg gttttgtatg
      781 aagacggttt ttgcggtggc cggcggcagg atgccactct tgcctgagag tacatccgtg
      841 tctttgatgt ttgatgaaca tccggtgaga agcagcgcac acatacacaa ggcatgcggt
      901 cgatacatca agtccccctc acacgcatag ggtgtggaat gtgtggcgct ccgtccggtg
      961 acggtgcagg tcctgacggg tgatttggga ggctattgtg cttgggagcc ccccaccacg
     1021 tcaatgacgt cgcgaccgtc ttttgccatc ccgttcgtct gtctcgtttg gttgaagccc
     1081 cactcgttgc cgtctgcctt agacgctcaa accccgttat ccctcacaac catcccttcc
     1141 gcttaaaata ccaatatgga gcaattccgg ataacaccat ggcgcacacg gccaggggat
     1201 atccccacca ccaatgcagt tccggcatca catcgaaatt catcccgtag atgctggcaa
     1261 tcaaggtcgg gggcagaaac accacagcgg caatggaaaa gatcttgata atttgatttt
     1321 gctcgatatt tatgagtccc attgcggcat tcattaaaaa attgaccttt tcaaacagga
     1381 aggcgttatg cggcagaaga gaatcgatat cacgtaaaat ttcgccaatc caatcgcgta
     1441 ccgtttcatc tccccgcccc cgccgaagga ggaaggctaa ggcgcgttgg gtatccatca
     1501 agcacagccg cactttccca ttcccatctt cctccctggt cagcaaatcg atcgccccct
     1561 gcagatctcg gccctccttc gccagtaccg tttgactgat ggtttccaaa gccaaatgcg
     1621 tggcttccaa ctgatcggcc agttgttcaa ttttcagttc aaacaacgcc aggagaaccg
     1681 acaatggatc ccggatcaga tccgattcat gtcgggctct tcgccgcatc agacgaaaca
     1741 acgggatggg atgatcgtgt aaggtaatga tgcaattgtc tttgatcgta aaggccaccg
     1801 ccctcgtggt ggcacgatga tcttcttcct gaaaaaagaa tgagtagata tggaggctat
     1861 cctctccttc attgacacgg gcactggctt caatttcccg cacctctttg ctggtgggca
     1921 atggttgctc gtacagcgtc tcaattattt gccgttcgtc gtccgtcggg gcagtgagat
     1981 cgatccacat ggccacgtca agagaattgg aaagctccgg gacgggtaaa gcctggaact
     2041 ttccatcatg taaggcatac gcgttgatca atgctcgatc ctctcttgtt cgcgagatcg
     2101 aaaaaagtat cccttcattg agatccttcg tgcaatcccc ggctcggtac caccaggccc
     2161 ctgcaacatg attgccggcg tgaccgcctc atcggagcca catccggatc agaagaccat
     2221 taccaaaagg ccggttttac agcaacggga ttcgatgaag caagcgatgc acccgtgggt
     2281 gaccgtcttg tgttacacgg ctcaaacggg gcgattctct cccggtgaag aagagccaag
     2341 ggaacggaag aacgcgaaca ttttcaactc cggtaagaat caggaataga tcacagatgg
     2401 aggaaggttc tagccctgct gacgggagcc ggtcttctta tgacccgatg aagtggttcc
     2461 agggccttaa tgcaccaatc ccgggagtgg ccccacctca acaagcgagg gagtgggtta
     2521 ctgataggag ttgaggtacg catgatgatc atgcatggtg gccccttccg gaagcaggtc
     2581 gtataatggg ccgagttttc cacgtatttt tctcgcgtaa aacttcggca attccaatgt
     2641 ttccccttcc agaaagtttc ggaccgacac atccgtatcc aataatcccc ggatcttcgt
     2701 gtggtatttg atccggccga acccttcact cgacaccgac cggataaaat taaaccactt
     2761 gggaatccag ccgcgattca gcatcatccg cttggcaatc atcggccagg aaaacgcatg
     2821 ctgtgacaga cccacgacat gatcataaaa ttccggccag gcataatgct taggacgcac
     2881 attcatcgca tggttgttat caagaaaatg aaaaggaaag ggtagcacgc gcccgtcccg
     2941 ttgcaattcc cgattaaagg gggctgcctg gccaaatgcc gtcagcaggg aaaaggccgg
     3001 gaaggccccg ggggctaaat ccaaaaattg cttggtgagg tcaaacggtt caggtccctg
     3061 atcagaatcc aagcccaaca caaaattcac ctgaacgtag gggatatacc gcaggatcaa
     3121 attgatatga tcggccacct gcttcacttt ttcttgtcct ctgtggcgac cggtccggga
     3181 tttatcgccc atgtcatacc acgactcaat ccccggcaaa atggccttga atccattttg
     3241 ttttaatcgt ttcaggtggg gttcggccag aagcgacagg gtgctttctg caatgaagtc
     3301 aatactgtta ggtggaaccg ccgtttcaat ggcattcata tattcttgaa accgcacccc
     3361 aaaattcggg tcgtgccagg ccacaatcgg tcgtttcaac tttgtccgca aaaatttgag
     3421 atcctcgctg atctggtcaa acgacaaagg ctgatagtcg acattggcgt ccacacaaaa
     3481 cccgcaggta tagggacacc ccatgctgcc gatcatcggc acgactttaa atgacgtggg
     3541 cgccttggcg atggtcggct caataaattt ccagcgctcc ttcgctccag gcaacgcggt
     3601 cggttgccgg gctgctgcaa gctgcactcc cagaggacga tgctgcgcac aatccgccag
     3661 aatatcctga atgagggtct tgtcggtaaa gcccaccaca tagtcgaaat acttagccgc
     3721 atcctgcgga tagcttcgcg catgaggccc gcccaatacc gtcacggccc cgcgtgctcg
     3781 aaacatctga ctgatggcat aggctgttaa cgccgatcgg gtaaacgccc cgatgaacac
     3841 aatatccgtc tcgtccaata attcctgctg gagatcttcc cggccggtgt agcacacaaa
     3901 ccgcacttga tggcccaatt cctcacacca caccccgacg acctgcggca tgatgctggc
     3961 cagattttga ttcatcaccc tggcatagag agaattcgtg ggacctttgg tgaccagatc
     4021 cagtatcgtc acccgacgtg ggcgcataga caacctccta aagtggggag aacagaaaaa
     4081 tgtggtggag ggggaaaaga ggggtctcct gaagctgttc taccgtagag gaaatcctgg
     4141 ataaggtcaa ggaaaaccag ttgcattagg gccgatcttc ctgtacggtt ttgtaagaat
     4201 cgaccgtctc gcaggattca agccattctc cggctgttct ggccggtgtc gtagcctaaa
     4261 cctcctcaga ccggcctcga tacgcaacct cagcatagaa caatgtcgtt cgtgcttcgt
     4321 ctggttgtgt ccaacaaagg aacc
//
LOCUS       DATIAL010000082         2465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_443886, whole genome shotgun sequence.
ACCESSION   DATIAL010000082 DATIAL010000000
VERSION     DATIAL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443886"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1065)
                     /gene="nuoH"
                     /locus_tag="VLA60_02690"
     CDS             complement(4..1065)
                     /gene="nuoH"
                     /locus_tag="VLA60_02690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSF08290.1"
                     /translation="MDTGIRLALTLSQIGVVMGIVLLTVAALTLLERKVLGWMQDRMG
                     PMEVGPYGILQPVADGLKLFFKEDIIPAGANKFMFSLAPVLSLVPALIGFAVIPFGPD
                     WTIEVGGVAFKPFVITDINIGILYILAFASIGAYGVILGGWSSNSKYSLLGGLRSAAQ
                     LISYELNVGLSIVGVLLLSGSLSLVTITNAQAGGFWNWYFFGGGSFPQAVAFVIFIIS
                     AVAETNRTPFDLPEAESELVAGFFTEYSGMRFAFFFLAEYANMILVSCVATVLFFGGW
                     HAPFEFLQGPESLYWVSGIFWFTLKVYTFLFLFFWLRATLPRLRYDQLMRFGWKVLLP
                     IALGNIVLTSIVAYIFPGH"
     gene            complement(1117..>2465)
                     /locus_tag="VLA60_02695"
     CDS             complement(1117..>2465)
                     /locus_tag="VLA60_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF08291.1"
                     /translation="DAYAAAILGMLKAVVEQGVLDPEMNTKAPQFLKDISAQLELISW
                     AGIEGMTGATTSVYKEAAETFAKGQKVIILVGPGILRLSGGFGMIVNLLDLLLLTGKL
                     SNPGCGLAPLAEENNDQGTFEMGGVGDVLPGGKSLRDSEARRTLSQTWGRPVPDSAGA
                     SLPQMLEAAQKGQLKALFIVGENPLGSLPPSSGIQQALESLEFLVCQELFLTETAKQA
                     NVVLPACSYAEKNGTFTNTEGHLQQVRRAIDPVGESRPDWEVFSALSVLMNESMEYLD
                     VKDIGKEIRNLLPGTRTLGPAPLPSKPDPSAIAHYISSEYKRDLASRYRMPSWTSTLS
                     DGVTLIIAQSLFHSGKYSLKAKGLMQIESKGKLYLHPEEAARRGLEDGDRVKVRNRLG
                     EVITTVGFRERIPHGMAVFPEHFDQDIRRLLPYAVDPETQVPYYRRTEVQIEKAPS"
BASE COUNT          677 a          687 c          564 g          537 t
ORIGIN      
        1 ttcttagtga ccaggaaaga tgtaggcgac aatcgacgtc aagacaatat tgccgagggc
       61 gatgggaagg aggactttcc agccaaaccg catgagttgg tcataccgta atctgggtaa
      121 tgtggctcgc agccaaaaga ataaaaacag gaatgtgtac accttaaggg tgaaccagaa
      181 gatgcccgaa acccaataca gggactcagg gccttgcaaa aattcaaatg gggcatgcca
      241 cccgccgaag aacaaaactg tggccacaca cgacacgaga atcatattgg cgtattccgc
      301 caggaagaaa aaggcaaacc gcatcccgct atattcagta aaaaacccgg ctaccagttc
      361 actttccgct tccggcaaat caaatggcgt gcggttcgtt tccgccaccg cggaaataat
      421 gaaaatgacg aaggctacgg cttgaggaaa ggatcccccc ccgaaaaagt accagttcca
      481 aaatccaccg gcttgcgcat tggtaatcgt caccagactc agagatcctg aaaggagcaa
      541 caccccgaca atggagagcc ccacattcaa ttcgtagctg atcaattgtg ccgcagaccg
      601 caggcctccc aggagagaat atttgctgtt tgaggaccac ccgcccaaaa taacgccata
      661 ggccccaatc gaggcaaagg ccagaatata gagaataccg atattaatat cggtgatcac
      721 aaacggttta aaggccaccc caccgacttc aatcgtccaa tcgggcccga aaggaatgac
      781 ggcaaatccg atcaatgcag gaactaacga cagcaccggc gccaggctga acatgaattt
      841 gttagcaccc gctggaatga tgtcttcttt aaaaaagagc tttaagccat cggctaccgg
      901 ttgcaggatg ccataaggcc cgacttccat cggtcccatc cgatcctgca tccaacccag
      961 caccttgcgc tccagcaagg tgagcgctgc gacagtcagg agcacaatcc ccatgacaac
     1021 gccaatttgg cttaacgtca atgctaatcg aatccccgtg tccatgacgg aaccactcct
     1081 atgatgtgaa acatctcatc gagtcctgac tcgacgttat gaaggtgctt tttctatttg
     1141 gacctcagtc cgccgataat atgggacctg tgtctccgga tcgaccgcat atggtaataa
     1201 ccggcgaata tcctgatcaa aatgctccgg aaataccgcc attccatgcg gaatacgttc
     1261 tcgaaatccc acggtcgtga tcacttcgcc taacctattc ctgactttca cgcgatcacc
     1321 atcttccaac cctcgacgcg cggcctcttc cggatggaga tacagttttc ctttactttc
     1381 gatctgcatc aatcccttgg ccttgaggga atattttcct gaatgaaaca gagattgagc
     1441 aatgatgaga gtcaccccgt cagaaagagt agaagtccaa gaaggcatcc gatatcgcga
     1501 cgccaggtct cgcttatact ctgagctgat atagtgggcg atggccgaag gatcaggttt
     1561 ggaaggtaac ggggcaggtc caagggtccg ggtcccggga agcagattcc ggatctcctt
     1621 cccgatgtct ttgacgtcaa gatattccat agactcattc attaaaactg atagcgctga
     1681 aaacacttcc caatccggac gactttcccc caccgggtcg atcgcccgtc ttacctgctg
     1741 aagatgccct tcggtattgg taaacgtacc gttcttttca gcataggaac acgcaggaag
     1801 aaccacattt gcctgcttcg cagtctccgt cagaaacagt tcctgacaga cgagaaattc
     1861 caggctctca agggcctgtt ggattcccga tgatggcggc aaggagccga gagggttttc
     1921 tcccacaata aataaggcct ttaattgacc cttttgtgcc gcttctaaca tctgaggcaa
     1981 tgaggccccg gccgaatcag gaaccggtcg tccccatgtc tgggacaaag tcctacgagc
     2041 ttcactatcc ctcaaggatt tcccacccgg aagcacatct ccgactccac ccatttcaaa
     2101 tgtgccttga tcattatttt cttcagccaa tggcgccagt ccacatcctg gattggaaag
     2161 ctttccagta agcagtaaga gatccaaaag attgacgatc atcccgaaac cacctgatag
     2221 acgaagaata cctggaccaa ccagaataat gaccttttgc cctttggcaa aagtctccgc
     2281 ggcctcctta tagacggagg tagtggcacc cgtcattccc tcaatcccgg cccaggagat
     2341 caactcgagc tgcgcgctga tatccttcaa aaattgggga gcctttgtgt tcatttcagg
     2401 atcgaggacc ccttgttcca caaccgcttt taacattccc agaatcgctg cggcatacgc
     2461 atcag
//
LOCUS       DATIAL010000083         6174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_336675, whole genome shotgun sequence.
ACCESSION   DATIAL010000083 DATIAL010000000
VERSION     DATIAL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336675"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VLA60_02700"
     CDS             complement(<1..652)
                     /locus_tag="VLA60_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08292.1"
                     /translation="MKHSKADTSQKAGLATLDAEGLAPQETPWEEFLRIFTKDYQALF
                     GFWVLVVLLLMALSGKVLTEWWVVFDPEAVRLTDKFLPPLSVPSPDSIPEDRPFGGVY
                     LMGTDELGRDVFARMFQGSFVSLSIGFVAVGISLGIGILLGGLSGFYGHVKLWFITVD
                     TLIMRFTDAMMCFPTFFLILTAVALLPPSIYTIMIIIGLFSWMGTARFVRAEFLSIR"
     gene            complement(681..1643)
                     /locus_tag="VLA60_02705"
     CDS             complement(681..1643)
                     /locus_tag="VLA60_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF08293.1"
                     /translation="MWNFIIRNIAQRLVLLVIVSFLAHSFIHLAPGEPSEVDPMNPRM
                     KPEDIAKIRAAFHLDDPLYMQYAYWIRDLASGELKSFKDSQPVLPKIWDRFLNSLPLF
                     ILATILVWTWAFPAGIYGAVFRGGLYDRLTVFSSYALISVPGFFLSFLAILWVVGWLG
                     VPVISIKTFGMEHAQPVYQLMDRIWHLVIPSVMTAIGGIAVLSRYVRSQMLEVLGQDY
                     VRTARAKGLQEDTVIYGHALGNALLPFVTMFGLLLPGLIGGAVIFEQIFAWPGLGRLA
                     YEAILSRDFPIIMTLNFIAAVLTLLGTLISDILYAVVDPRIRLD"
     gene            complement(1672..3816)
                     /locus_tag="VLA60_02710"
     CDS             complement(1672..3816)
                     /locus_tag="VLA60_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF08294.1"
                     /translation="MFVKRLLLFIPLTLVLFLVQSYFWVPTYEHQAAGNPDRLITYIE
                     ASSGDAKILNPILNADSASSNIVSHVFEGLLDLDEDLKLRSRLATDWQITEQAYLLVN
                     PHHRFPDGTEVTGSKLFRKIHEAWKAGTIEGLQERVQSIELLPSGQRSEPISLLVPNA
                     EGKPQLKDLSVTIDVPERVAFTLSEVDQDLFDRLKPVLGERYFEHFPYNKFIHPQEPV
                     SKELEEPLRAKFPDILPVGEHNPTILFHLRQGVKFHDGHVLDAGDVKFTYESIMNPKN
                     LSPRTPDFEPIKTVNILDPSTIQIVYKRLYSPAINAWTMGILPEHLLNAEALAREKQE
                     RGLSEEAQKTFGMRESLFNRHPIGSGRFEFVEWQGDEFIHLRRYEDYWEGPAEYHEYY
                     MRIIPELFTQEVEFRTGAVDFYAAQPHQVDRYKNDPTYQWFSSLGFAYTYIGYNNRKP
                     LFADPKIRTALGMAINVDEIITYLVYGEGERTTGPYPRNTEWYDQTIQPLPYDPQGAV
                     AIFENAGWKMNRDGWLEKDGKIFEFNLITNNGNPIRKNLMTIAQNAWKKIGVKVNTQV
                     FEWAVFLNDFVNTGDFDAVVLGWSMGIDPDLYQIWHSSQAGPQQLNFVGYNNPRADAL
                     IVRIRQEYNRDRQEQLTHELHRLIHEDQPYTFLYAPLSTRVLDKKIVLVEKGPDGKEQ
                     FKKIYPTKSGDITFYFHKWRKLEFTPKFSLDG"
     gene            complement(4190..4498)
                     /locus_tag="VLA60_02715"
     CDS             complement(4190..4498)
                     /locus_tag="VLA60_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSF08295.1"
                     /translation="MSELVRIAKIEEVEEGKGMVVEAGEKCLAVFKVAGAFHVIDNTC
                     LHRGGPLGEGDVEGETVTCPWHGWEYNVKTGHCLTNPSSHVRSYPTVVEDGEVKVDLS
                     "
     gene            complement(4529..5425)
                     /locus_tag="VLA60_02720"
     CDS             complement(4529..5425)
                     /locus_tag="VLA60_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSF08296.1"
                     /translation="MAAEQNPAGPATPPNQPVGNPLPGVKHIIAVSSGKGGVGKSTVT
                     VNLAVALKQQGYAVGLMDADVYGPNIPLMIGVSKEPGKDGDKILPAEGQGVKVISMGF
                     FVPEDTPVVWRGPMVHSAIQQFFRDVVWGELDYLLIDLPPGTGDVPLTLSQLVPLTGA
                     ITVTTPQEVALQDVRKGMTMFKKVNVPLLGVIENMSYFVCGHCHERTEIFSTGGGERA
                     AQKFEIPFLGRIPIDPAIREGGDKGTPIVSNDPSSPQAQAFLQIAKTLTANIDQAEKG
                     NGDTAPSISSLLKKITDPLKKS"
     gene            complement(5629..5832)
                     /locus_tag="VLA60_02725"
     CDS             complement(5629..5832)
                     /locus_tag="VLA60_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08297.1"
                     /translation="MDCPRCQGIMIQDKFGDVADESGAIYFSGWRCITCGEILDPVIS
                     ENRRHHHEPLIGRSRKKFATQLG"
BASE COUNT         1716 a         1635 c         1377 g         1446 t
ORIGIN      
        1 cacgaatgga taaaaactcc gcccgcacaa atcgggccgt ccccatccag ctaaacaatc
       61 caataatgat catgatggtg taaatgctcg gcggaagtag agccacggca gtaagaatga
      121 ggaaaaatgt cgggaagcac atcatggcat cggtaaatcg catgatcagc gtatcgaccg
      181 tgataaacca caatttcacg tgcccgtaaa aaccggacag tccgcctaat aagatgccga
      241 tacccaggga aattccgaca gccacaaatc caatggataa agacacaaac gaaccctgga
      301 acatccgggc aaacacgtca cgtcccaatt cgtccgtgcc cataagatag acaccgccaa
      361 acggcctgtc ctcgggaatg ctatcaggag aaggcacaga cagcggagga agaaatttat
      421 ctgttagtcg tacggcttcg ggatcgaaca cgacccacca ttctgtcagc actttcccgg
      481 ataacgccat cagcaagagc accacgagga cccaaaaacc gaagagggcc tggtagtcct
      541 tggtaaatat gcgaagaaat tcttcccacg gcgtttcctg cggagccaga ccttccgcat
      601 cgagggtggc caacccggct ttttgtgaag tgtcggcctt cgagtgcttc atgaagaaat
      661 cgttcctccg gttctcgtaa tcaatccaag cggatccttg gatccaccac cgcatagaga
      721 atatccgaaa tcaatgtgcc caataaggtc agaactgcgg cgataaagtt gagcgtcatg
      781 atgatgggaa agtcccgtga aagaatggct tcataggcta accgtcccaa ccccggccag
      841 gcaaaaatct gttcgaagat cacggccccg ccaatcaacc cggggagcag aaggccgaac
      901 atggtcacaa acggtaatag agcattcccc aacgcatggc cataaatcac ggtatcttcc
      961 tgaagacctt ttgccctggc ggtccggaca taatcctggc ccaacacctc caacatctga
     1021 gaacggacat agcgagacag cactgcgatc cccccgatcg cggtcatgac ggatggaata
     1081 accaaatgcc agatacgatc cattaattga tagacgggct gcgcgtgctc catcccgaaa
     1141 gttttgatac tgatcaccgg aacccccaac catcccacta cccacaaaat ggctaaaaac
     1201 gacagaaaaa accccggaac cgaaataagc gcatacgacg aaaacacggt cagccggtca
     1261 tacagtccgc ctcgaaagac cgccccgtaa atcccggctg ggaaagccca tgtccacacc
     1321 agaatcgtgg ccagaataaa caaaggcaag gaattcagaa atcgatccca aatcttaggg
     1381 aggaccggtt ggctgtcctt gaaagactta agctcccccg aagccaagtc ccttatccaa
     1441 taggcatatt gcatatagag tgggtcatct aaatgaaaag ccgcgcgaat cttggcgata
     1501 tcttccggtt tcatccgcgg attcatggga tccacttcac tgggttcccc tggggccaga
     1561 tgaataaagg aatgggcaag aaaagacaca atgactaaca ggaccaatcg ttgagcaata
     1621 ttacgaataa taaaattcca catagtggcc agccgtcaga ctatgtcgtc gttatccatc
     1681 aagggaaaat ttcggcgtga attcgagctt ccgccattta tggaaataaa acgtgatgtc
     1741 cccgcttttg gtgggataaa tctttttaaa ctgctctttc ccatccggtc ctttttcgac
     1801 cagcacaatt ttcttatcca agacccgcgt actcaacggg gcatagagaa aggtataggg
     1861 ttggtcctca tgaatcaacc gatgaagctc gtgcgttaat tgctcttgtc gatctctatt
     1921 gtattcctgg cggatccgga caatgagggc gtcagcgcgc gggttgttgt accccacaaa
     1981 atttaattgt tgcggcccgg cctgactgga atgccaaatc tgatagagat cgggatcgat
     2041 gcccatgctc cagcccaaca caaccgcatc aaagtctccg gtattcacaa aatcgttcaa
     2101 aaacacggcc cattcaaaga cctgtgtatt cactttaaca ccgatctttt tccaggcgtt
     2161 ctgcgcgatg gtcatcaaat tttttctgat agggttcccg ttattcgtaa tgaggttaaa
     2221 ctcaaaaatc ttcccatctt tttcgagcca cccgtcccta ttcattttcc atccggcatt
     2281 ctcgaaaatc gcaacggctc cttgagggtc gtaaggaaga ggttggattg tctgatcata
     2341 ccattcggta tttcggggat agggcccggt cgtccgttcg ccttctccat acacaagata
     2401 ggtaatgatc tcgtcaacat taatcgccat ccccagagct gtcctgatct tcggatccgc
     2461 aaataacggt tttcggttgt tatagccaat ataggtatag gcaaagccca ggctgctaaa
     2521 ccattgataa gtcggatcgt ttttataccg gtcaacctga tgaggctgag ccgcatagaa
     2581 atcgaccgcg cccgtacgaa attccacttc ttgtgtgaac agttcgggaa tgatgcgcat
     2641 ataatattca tgatattcag cgggcccttc ccaatagtcc tcataccgtc gaagatgaat
     2701 aaattcatct ccctgccatt cgacaaactc aaagcgcccg ctgccaatgg gatgtcgatt
     2761 aaacaaactc tctcgcatcc cgaatgtctt ttgagcctcc tccgacaatc cgcgctcttg
     2821 tttttcacgg gctaaggcct cggcattgag aagatgctcg gggaggatcc ccatcgtcca
     2881 cgcattgatg gccggggaat agaggcgttt atagacaatt tgtatggtcg agggatccag
     2941 aatattcacg gttttaatcg gctcgaagtc cggagtccgt ggagacaaat tttttggatt
     3001 cataatggat tcataggtaa acttcacatc tccggcgtcg aggacatgcc catcgtggaa
     3061 cttcacgccc tggcgaagat ggaacaggat ggtgggatta tgctcgccca cgggaagaat
     3121 atcggggaat ttggcccgca ggggttcctc caactccttg gaaaccggct cttgaggatg
     3181 aatgaatttg ttataaggaa agtgctcaaa gtaccgctca ccgagaaccg gctttaagcg
     3241 gtcgaataaa tcctggtcca cctccgagag cgtaaatgcc actcgttccg gtacgtcaat
     3301 ggtcacggag aggtctttga gctgaggttt gccttccgca ttgggcacta agagtgagat
     3361 gggttccgat cgttgcccgg aaggtaataa ttcaatggat tggaccctct cctgcaaccc
     3421 ctcaatggtt ccagccttcc atgcctcatg aatcttccga aaaagcttac tccctgtcac
     3481 ttctgttcca tccggaaagc ggtgatgcgg gttaaccagg agataggctt gttcagtaat
     3541 ctgccaatcc gtggccaatc ggctccgaag cttcaagtct tcatctaaat ccagcagccc
     3601 ctcaaacacg tggctcacaa tattggagct ggccgaatcc gcgttcaaga taggattgag
     3661 aattttggca tccccgctgg acgcctcaat ataggtgatt aagcgatccg gattaccggc
     3721 agcctggtgc tcataggtag gaacccaaaa ataggattga accagaaaaa ggaccagagt
     3781 caacgggatg aacagcaaaa gacgtttgac aaacatagaa gtctacatca acaccacaca
     3841 gttaatacct caatgttatc aagaacccaa aggggatgct atgcctgatt gattgtaatc
     3901 aaaaaatcat gacaccggat gtgaaatatg ctctctaaaa tttaatacgg ggaaagggaa
     3961 taaacacgag acgatggcca agaccagaca tgcggattaa ctgtctgatt ggatctgact
     4021 gggccaagaa ggcatcaaaa aagatcgcat tgactcatga ggggaatgcc acacatgacg
     4081 gagatgtggg cgcgtgaggc cttactacaa gccgcaagtt tcccgccatt tgcgatgtgg
     4141 gatagccgct cctggccacc gtgccactat acaatggcct tttctatgcc taggacaaat
     4201 cgactttcac ctctccatct tcaacgacgg tcgggtagga tctcacatgg ctggagggat
     4261 tcgtcagaca atgcccggtt ttcacattgt actcccaccc atgccagggg caggtcaccg
     4321 tctccccctc gacatcgccc tcacctaaag gaccgcctct atggagacag gtgttatcaa
     4381 tgacatggaa ggctccggcc actttaaaga cggccaaaca tttctcaccg gcttccacca
     4441 ccattccttt tccttcttcc acttcttcta ttttcgcgat acgcactaac tccgacatag
     4501 gttctcctca acatgaatat gctttgattt aagatttttt caagggatcg gtaatttttt
     4561 tgagtaaact ggaaatggag ggcgcagtgt ccccgttacc tttttccgct tgatcgatat
     4621 tggccgtcag ggttttggca atctgaagaa aggcctgggc ttggggcgac gagggatcat
     4681 tgctgacaat tggagtaccc ttatctcccc cctcccggat ggccggatca atcggaattc
     4741 gtcccaaaaa tgggatctca aatttctgag cggcacgttc cccaccgcct gtcgaaaaaa
     4801 tttctgtccg ttcatggcaa tggccacaga caaaataact catattttca atcaccccga
     4861 gcaacgggac attcaccttt ttgaacatcg tcatgccttt tctcacatcc tgtagagcca
     4921 cttcctgcgg agtggtcacg gtaatcgctc cggtcaacgg cactaattgt gacaacgtca
     4981 acggcacatc tcccgtccct ggcggtaaat cgatcaataa ataatcgagt tctccccaga
     5041 ccacatcacg aaaaaattgc tggatcgccg aatggaccat gggtccccgc cacaccaccg
     5101 gcgtgtcttc cggcacgaaa aatcccatgg aaatgacttt cactccctgt ccctcggcag
     5161 gcagaatttt gtctccatcc tttccaggct cttttgacac cccgatcatc agaggaatat
     5221 tcggtccata cacatccgca tccattaatc ctaccgcata gccttgctgc ttaagcgcga
     5281 ccgccaagtt cacggtgacc gtggatttgc cgactccgcc tttgccactg ctcaccgcga
     5341 tgatgtgttt cacgcccggc aatggattgc ctacaggttg attgggtggg gtcgcgggac
     5401 cggccgggtt ttgttcagca gccattcagt aatctcctta tgtcacagac actcataata
     5461 aattcacata tgcgatgcag taggccttca ccacttctcg ccacaccatt agaccatgaa
     5521 tcaaagagaa cccgaaaccc tcccagactc aagaggattt cgggtctctc accacccatg
     5581 cacaattgac agtagggata cgaacaaaag aacgacgggc agcaacacct accctagttg
     5641 cgtggcaaac tttttacgcg atcgcccgat gaggggttca tgatgatgcc ggcgattttc
     5701 agaaatcacc ggatccaaaa tttccccaca ggtaatacac cgccaacccg aaaaatatat
     5761 ggccccggat tcatcagcca catcaccaaa cttatcttga atcattattc cctgacaacg
     5821 cggacaatcc ataaaaagca ctccttctcc caggaaaaag aattataaaa tcaatgcgta
     5881 attaattagg tagaaaagca atccatgtgc cgtaataaat tacgcataag cacttgataa
     5941 ataatgaatt tacataaagc atccttccat aaacgtttca gaatgaaacc aatacaatta
     6001 aattacacga attaaatctg cataaactat tgaaaatata tcactttaat acaaatgaca
     6061 caattaggta acaacgtgga ccaaattgaa acgtctttag gttgtttgag ttattccaaa
     6121 ttgtttgacc tttcgccaca aggtcgatcg accgattcct aactgttgag aagc
//
LOCUS       DATIAL010000084        14332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1519722, whole genome shotgun sequence.
ACCESSION   DATIAL010000084 DATIAL010000000
VERSION     DATIAL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519722"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VLA60_02730"
     CDS             complement(<1..1031)
                     /locus_tag="VLA60_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HSF08298.1"
                     /translation="MDDWKKVLAASITKPKDLAKYLGVDPKEVEAVVGPYPMRITPTV
                     LATIKSKGDAIWKQVVPEAIELDDIDAPDDPLEEDTDSPVPHLVHRYPDRVLLMVTNQ
                     CPIYCRFCTRKRLVGKPGFLKKGELDRAVAYLREHTEVRDVILSGGDPLLLPDRLIER
                     ILKGLRSIPHLELIRFGTRVPGTLPQRITPELCEIVKRFHPIYMNLHFNHPDELTPEV
                     KEACGRLADAGVPLGAQTVLLKGVNDDPEIMKRLMHQLLLARVKPYYLYQADLTKGTN
                     HFRTTVETGLKIIQSLQGHTSGMAVPHFVIDAPGGGGKIPLLPNDYLLNLDENGAVLK
                     NYENKTYHYP"
     gene            complement(1035..1346)
                     /locus_tag="VLA60_02735"
     CDS             complement(1035..1346)
                     /locus_tag="VLA60_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSF08299.1"
                     /translation="MSDTLNRMKLYHTEWCPECQVVRERLAELGLPYEDEVVPDVRPF
                     RKNLYEVSGQNYVPVLVHGETVLTETSDILAYLDDYEDHGIPSGEATKPPSSNLDERN
                     H"
     gene            complement(1415..2911)
                     /locus_tag="VLA60_02740"
     CDS             complement(1415..2911)
                     /locus_tag="VLA60_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08300.1"
                     /translation="MPGPLHPSSTINPGSVISSHVEQSGPILPLSPSISDSPYPLFRD
                     RLIKDGLFVKGQEDHWRVACGPFSLSTEEAKFFEALGQHLLVFYQALNRLYFESLKGL
                     QPAWVHEYLDIGKPEDLLVLARMNRFKNHLPGVIRPDLIPTNAGMALTELDSVPGGIG
                     LTGSLSRMYAQQGFPVWPQDDGLVQGFSRMLRGALHGRPPRVAIVVSDEAEAYRAEMQ
                     WVVTQLRQEGWEAYCGHPRDIRFTEEGLLIHHEGQEQPLSVVYRFYELFDLKNIPKGE
                     LIAYSAKKGRVVVTPPYKPWMEEKLALALFHHPMLEKFWEHELKSDTFDFLRAIIPET
                     WILDPRPLPPSAVIPGLRHGNRAVADWQWLGEATQKERQYVIKVSGFSDQAWGSRGVS
                     IGHDMSQVQWKEVVSHALDQFETSPSILQTFHKGRLVVVEYYDSHSGDVVSMEGRVRL
                     SPYYFVNEGQARLGGVLATVCPKDKKIIHGMRDAVMAPCSIMASHLSS"
     gene            3108..3731
                     /locus_tag="VLA60_02745"
     CDS             3108..3731
                     /locus_tag="VLA60_02745"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSF08301.1"
                     /translation="MPQAIPIIPGPQVVPSAVCFRCDVCCRFPEQDSILRPYFTEDEI
                     RQAVTHGISHSSFPDYRGSQIEVVRHPTDEGFLCPAFDPITQHCRIYEVRPLDCQLYP
                     FALMWNAQHERVVLGWDTLCPFLLEQAGEENPLRRAGLEPSALTLPKAFMEQAQRIAN
                     YLESDNVLNSLAVHPRLVTPFQPDVVVLQTLDRLTATLRSSNMRDTR"
     gene            3787..4743
                     /locus_tag="VLA60_02750"
     CDS             3787..4743
                     /locus_tag="VLA60_02750"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HSF08302.1"
                     /translation="MAIHLSPLTIKDQVRLNQAIASTCIGGKTPLSTWSFPPHYIWKD
                     LFTFSWTDIDGWLCLFAEYSDGIFMPLPPVGPWSDIGSSSSSPLKNVLSQAMAYMETR
                     NRGSNVTRIENIPADLKEEIQEWGYTLTPKDSDYLYRTADLIHLKGNPYKSQRAACNR
                     FIRAHRIRIAPYQIIDRDACFTLFHRWVHQKEDIPISLLGPNEDVARMMLRDAANAHR
                     VTLQEYRELGLTGRIVWVDGAVKAYTFGFPRSLDVFCVLLEVADRSVYGLAQYLFREF
                     CREFQAYSFVNTMDDSGLPSLARTKLAYHPCQLVPNYIAQPS"
     gene            5132..5944
                     /locus_tag="VLA60_02755"
     CDS             5132..5944
                     /locus_tag="VLA60_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HSF08303.1"
                     /translation="MKGLEFGIEQFRVTDEPFYQEVNGEIDLFTVGAKNKIPVMLKGP
                     TGCGKTRFVQHMAHRLNRPLITVACHEDLTASDLIGRYLLKGEETVWVDGPLTLGVKH
                     GALVYLDEVVEARKDTTVLIHPLSDDRRFLPIEKKGQMIQAVDDFLLVISYNPGYQSV
                     LKELKQSTKQRFMAIEFGYPPKDIETRIVEHEAKVSRDVAIRLVKLGEKVRNLRNHGL
                     EEGVSTRLLIYAGTLMQQGVAPDRACDAAITRPITDDTDMQRSIQELVKAIF"
     gene            6354..6692
                     /locus_tag="VLA60_02760"
     CDS             6354..6692
                     /locus_tag="VLA60_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF08304.1"
                     /translation="MKPKMTNHPMYTLLREGKITEFNARFKTGEKPDLSNCDFRSVDL
                     RGIEVAGMDFRGSYFRQADLRGVDLSRCNLEGASIHGTKISGTLFPKELSAQEILLSN
                     QQGTRMRYGV"
     gene            6864..7742
                     /locus_tag="VLA60_02765"
     CDS             6864..7742
                     /locus_tag="VLA60_02765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF08305.1"
                     /translation="MNQPQNIIAIVYDFDHTLSPHYMQDHTILRHAELDPSTFWKSCT
                     ALIEARDYDQELAYMKRMLEEPRIRMLSNQDLRDMGNQLSFFPGVPSFFEELNQIFKK
                     PKYEEVPIRLEHYVVSSGLKAILEGSVVATQVRAIFGCEFDEEDGHISFPKRTISHTQ
                     KTQFLFRVNKGLTDLKEDVNDHMPEESRRVPFRQMIYVGDGPTDVPCFTVMKKNGGFA
                     LAVYNPEDQTRRSFEKCYQLTFHADRVHFMAPADYRPGSHLRLILEKHIAEVADRIVD
                     SRRQGIEGSRVPAPLP"
     gene            7879..8514
                     /locus_tag="VLA60_02770"
     CDS             7879..8514
                     /locus_tag="VLA60_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08306.1"
                     /translation="MHIVESFFMKLFRKSVGGSCFLRTVTALVLIGLLSSISWADDPF
                     GSSTLAPNKNDMIKDMAFDQVNWKRPQGVFTWITMATGYDTPWDSLGRPGWMTTGDAY
                     VEPLNPGVSEFPLDTQTFYIVFEAQPLDAPGQFAAAWFQMNDGQVTSNTPVGKDVIEL
                     EMNQRSGYFQISKPAEGWKPGKYQVKIFYGSPGQALHAVNVIGTMEFTIKG"
     gene            complement(8692..10323)
                     /locus_tag="VLA60_02775"
     CDS             complement(8692..10323)
                     /locus_tag="VLA60_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF08307.1"
                     /translation="MNSVLYVFLPCKKVYPTGITYLADFIHRRRPDVRQQILDLSLFP
                     PDQRQAQLREVTKAFQPELVCFSWRDIQIFSPHEGDASLEHAFNFYYASNPLKRVVAS
                     FQGLQNLYRYYTDIRSNLSYPWLVQKEFPSIGMMIGGGAFTAFADQLIEKLPEGIIGI
                     LGEGEDAILKVLNGEPLGDERFIIKEQGRVTKGTKNTPALLDALSVDITYLTSIFPQY
                     REYIGECIGVQSKRGCPYDCAFCLYPYIEGKRVRYRPADNVVKDIAQYYHQWGARRFW
                     FTDAQFITGKDAYPQCTEILERIISQQLHIEWSGYVRTSLINPKLAKLMVQSGVGDLE
                     VAITAGSQKVLNSMHMGFKLEHLYEGCRYLKEAGFNGRVILNYSLNSPDETEETLLQS
                     IESYKIVASILGEDRVFPLMFFLGIQPNTDLEQRLLSEGYLSKGYNPLSLTPWNIKKM
                     LYNPAPLNKIIAKACLAAWGRKHGSQDPRPWSGSLSQSATQQQGHTYADTSLSKGFET
                     NSGRDALLTLEEILRSRRPSNSQPLSPAPEPNPSR"
     gene            complement(10328..11077)
                     /gene="ubiE"
                     /locus_tag="VLA60_02780"
     CDS             complement(10328..11077)
                     /gene="ubiE"
                     /locus_tag="VLA60_02780"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HSF08308.1"
                     /translation="MNKIDIPGKAASTWTGPAREQAVQRMFTAIAKFYDLNNSLLSFG
                     LHHSWKRKAISYIPDIPGTRALDLGAGTCDLAILVDQHLHYTSQIIAADLNLAMLRVG
                     QDKVRSQSLANRITCLQMNAEHLTFPSATFDTVTAGFCIRNVGNLPQALLEICRVLQP
                     GGRFVCLEFSRPVSAGLRKLYDWYSFHLLPWIGTKVARDGTGVYEYLPASIRNFPDQN
                     RLAQLLKEAGFQSVDYHNLTGGIVAIHVAVK"
     gene            complement(11202..11621)
                     /locus_tag="VLA60_02785"
     CDS             complement(11202..11621)
                     /locus_tag="VLA60_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08309.1"
                     /translation="MGVKPRFVIYAHNATYDKLHQVATLGLTASAMGKDVMVVLLFWT
                     IKKLAEGRLNELDFPPEYAAQAPEVARLIRERKVPLISDMFEEARQVGQFKLVACSAG
                     LEYMGVDAKAVTQGVDEVMGLPAIISACSGAETTLFI"
     gene            complement(11921..12646)
                     /locus_tag="VLA60_02790"
     CDS             complement(11921..12646)
                     /locus_tag="VLA60_02790"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HSF08310.1"
                     /translation="MVQESEDQREYIEEVAVVGVKVRDLGEVKKTRTDDHTVKVGDWV
                     LLDLDNDHTFGKVYLPPQFLPFTPPMRVMRQIIRKATEEDERQIARQLRLAQEGEEFC
                     QERIAELGLGMTLVEVFGSFAQRSLTFTYTADDRIDFRQLVKDLARRFGCRIEMRQIS
                     SREEAARLSGVDTCGLVLCCSSFLTDFKPVYPRGRADAPMNGMDSHRIGVCGRMKCCL
                     LYEEEGWTLTRRKPQPLIRPTRR"
     gene            complement(12783..>14332)
                     /locus_tag="VLA60_02795"
     CDS             complement(12783..>14332)
                     /locus_tag="VLA60_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF08311.1"
                     /translation="STEPPGPFPFLGGVDPEVVSSLHQVHEIMMSGIETAISDIMAKR
                     ASVDDPQLSRRLEDAYAEVVQSRPSLQRHIQCGRKSDGTFHWDYPKNPTASAKMTYGG
                     LRIFNSVTRQAIPFGFERPIGPNVGHFLGFLTGRHTMVEIQTVAQAGGRDLERQLAQL
                     FTLLKDHDCLAIAPNTSLEAHWRKVTRDQDVVHLGHAALMYRHQDNFLWFDPWLMPWF
                     AESPVPSLWADLLPRPAGIFLTHDHDDHVDPRTLYHLPKDVPIFVPSRRNRKALHYDY
                     LSLLRELGFSQVRELAHGESWRIGEAQVVSVPFFGEDPCDVELPRNCYLVADRGRNTL
                     VHADSGPTNDGRSALQDKVMANLVSKYGPIPLVFASQQQLKEVRSYAAYACLSPPGTW
                     LEVGENGFLTNAYLAELCRTAQAKLFVSYATGGADWYPDHLSFMFSGRNPARTALLTA
                     NWDPPESLRQELEPFGCRYHFGQAFDVFGAGPNGETLVSHLVDQLTPIGLYQLDHAPP
                     PFLRKHR"
BASE COUNT         3576 a         3878 c         3476 g         3402 t
ORIGIN      
        1 ggataatggt aggttttatt ttcgtaattt tttaatacgg ccccgttctc atccaaattc
       61 aataaataat cgttgggcaa cagaggaatc tttccgccac ctccgggcgc atcaatgaca
      121 aagtggggaa cagccatgcc actcgtatgc ccctgaagag attgaatgat tttcaacccc
      181 gtttccaccg tggtccggaa atgattcgtc cccttggtga gatcggcttg atacaggtaa
      241 tagggtttca ccctggccaa caacagttga tgcatcagcc gtttcatgat ttccgggtca
      301 tcattgacgc ctttcagcaa aacggtttgg gcacccaagg gcaccccggc atccgccaat
      361 cgaccgcaag cctccttgac ctccggcgtc aactcatccg gatggttgaa atgcaaattc
      421 atataaattg gatggaagcg cttaacgatc tcacacaatt ctggagtaat gcgttgaggg
      481 agagtacctg gcacgcgggt cccaaaccga atcaattcta aatgcgggat ggaccgaagg
      541 cctttcagaa ttctctcaat gagacgatcg ggcagtaaca aagggtcacc gccggacagg
      601 ataacatccc gtacttcagt atgctctctg agatacgcca ccgctcgatc cagctcaccc
      661 tttttgagaa accccggttt ccccaccagt cgctttctcg tgcaaaaccg acaatagatt
      721 ggacattggt tggtcaccat caacaatacg cgatcgggat atcggtggac caaatgagga
      781 accgggctgt ccgtatcttc ttccaacgga tcgtccgggg catcaatatc atccaattcg
      841 atggcctcgg ggactacctg cttccagatc gcatcccctt tacttttaat cgtggccagc
      901 acggtcgggg taatgcgcat tggatagggc ccgacgacgg cttccacctc cttgggatcg
      961 acccccagat acttggctaa atccttgggt ttggtgatac ttgcggcgag gacctttttc
     1021 caatcatcca tagcttaatg attcctttca tcaaggttgc tggagggtgg cttggtggct
     1081 tccccggagg gaatcccatg gtcttcgtag tcgtccagat atgccaggat atcggatgtc
     1141 tccgtcaaca ccgtttctcc atgaaccaac accgggacat aattctgtcc cgagacttca
     1201 tacaaatttt tccggaaagg tcgaacatcg ggcacaactt catcttcgta gggcagaccc
     1261 aactcagcca acctctcgcg gaccacttgg cattccgggc accattccgt gtgatacagt
     1321 ttcatgcggt taagagtatc agacatgtca acttggggtc ttccccatga gaggaaaaat
     1381 ataccaggac cgaaagaacc aggcgccctg ggccttatga gcttaggtga ctagccatga
     1441 tggaacaggg agccatgacc gcatcccgca ttccatgaat aattttttta tctttgggac
     1501 ataccgtcgc caggacaccg cccaaccggg cctgtccctc attcacgaaa taatacggag
     1561 ataagcgcac ccgtccctcc atactgacga cgtcaccgga atggctgtcg taatattcaa
     1621 ccacaaccag tcgccctttg tgaaatgttt ggagaatcga agggctcgtc tcaaattgat
     1681 ccagagcatg ggacacgacc tctttccatt ggacttgcga catatcatgc ccgatcgaaa
     1741 cgccccggct cccccaagcc tggtcggaaa atcctgacac tttgatgaca tactgacgtt
     1801 ctttttgggt ggcttctccc aaccactgcc aatccgccac cgcacgattt ccatgacgta
     1861 ggccgggaat cacagcagaa ggcggaaggg gtctgggatc caaaatccag gtctcgggaa
     1921 tgatggcacg aagaaaatca aacgtatcgg atttcaattc gtgctcccaa aatttctcca
     1981 gcatcggatg gtggaacaac gctaaggcca gtttttcctc catccagggc ttatacggcg
     2041 gggtgaccac aacccggcct tttttggcac tgtaggcaat caattcccct ttgggaatat
     2101 ttttcaaatc gaacaattcg tagaaccggt agaccaccga aagaggctgt tcctgtccct
     2161 catggtgaat caaaagaccc tcttccgtaa atcgaatatc acggggatgt ccgcaatagg
     2221 cctcccaacc ttcctgccgg agttgggtca ccacccattg catttcggcg cgataggctt
     2281 cagcttcatc cgagactaca atggccaccc gaggaggccg cccgtgcaat gcccctcgta
     2341 acatacggga aaacccttga accaacccgt cgtcctgcgg ccacacagga aagccctgct
     2401 gggcatacat ccgactcaaa ctgccggtta agccaatccc tccagggacg gaatcaagtt
     2461 ccgtcagggc catgccggcg ttagtgggaa taagatcggg acgaatgaca ccgggaaggt
     2521 gatttttgaa tcgattcatc cgtgcaagga ccagaagatc ctccggcttt ccaatatcca
     2581 aatattcatg aacccacgca ggttgcagac ctttcaggct ttcaaaatac aaccggttca
     2641 acgcctgata gaacaccaac agatgctgcc ctaatgcttc aaaaaatttg gcttcctcgg
     2701 tggaaagcga aaagggtccg catgccactc gccaatgatc ctcttgcccc ttcacaaata
     2761 atccgtcctt gatcagcctg tcccgaaaga gaggataggg gctgtcagat atcgacggac
     2821 tcaatggcag aatggggccg gattgttcta cgtgggaaga aataaccgat cctgggttaa
     2881 tcgtggaaga cgggtgtaac ggaccaggca cgctgacgtc cttcactcgt cgatcacaag
     2941 aggttggacc tgcacgaaat acttcacggg cctaacatga aatgcttgga ccttaatgac
     3001 ggcccttttg ctagaatagg ggagatcgta gcacgctctc ttgaaaccag acaaggcacg
     3061 cacccgatgg aaatggcggg ccaagtcgcc atgaaagtta tacgcctatg cctcaggcca
     3121 tccccataat tcctggtcca caagtcgttc cctcagccgt ctgttttcga tgcgacgtgt
     3181 gctgtcgttt ccccgaacaa gacagtatcc tcagacccta ctttacggaa gatgaaattc
     3241 gacaggccgt cacgcacggc atctctcact cttcctttcc tgattatcgg ggtagtcaga
     3301 ttgaagtcgt tcgacatccg acggatgaag gatttctctg tccggcattt gatcccatca
     3361 cacaacactg tcgtatttat gaggtgcgtc ccctggattg ccaattgtac cccttcgcgc
     3421 tcatgtggaa tgcccaacac gagagggtcg tacttggttg ggatacgttg tgcccgtttc
     3481 ttcttgagca agccggggaa gaaaaccctc tcagaagagc cggcctagaa ccctcagcat
     3541 tgacccttcc aaaggcattc atggaacagg cccaaagaat tgcgaactat ctggaatctg
     3601 acaacgttct aaattccctt gccgttcatc ctcgattagt gactccgttt caaccggatg
     3661 tcgtcgtact gcaaaccctc gaccgcctga ctgcaactct tcgttcctcc aatatgcgcg
     3721 acacgcggtg acggccacag actcgctctt ttctcacaaa ccctccatcc cgtataacta
     3781 tcgttcatgg ccattcatct cagtcccttg accatcaaag atcaggttcg tctaaaccag
     3841 gccattgcct ccacatgtat cggggggaaa acccccttga gcacctggtc atttccaccg
     3901 cattatatct ggaaagacct gtttaccttt tcctggacgg acattgacgg ttggctatgt
     3961 ctcttcgcgg aatattccga tgggatcttt atgcctctgc ctcctgtcgg accttggtca
     4021 gacatcggat cctcatccag cagccctctc aaaaacgtgc taagtcaggc gatggcgtat
     4081 atggagaccc ggaatcgtgg atccaacgtt acgcgaattg aaaacattcc cgctgattta
     4141 aaggaagaga ttcaagaatg gggatatacc ctcacaccaa aggactccga ttacctctat
     4201 cgcactgccg atctcatcca tctgaaagga aatccctaca aatcccaacg cgccgcgtgc
     4261 aatcgattta ttcgcgcaca ccgcatccgg attgccccct accagatcat cgatcgtgat
     4321 gcgtgtttca ccctttttca ccgttgggtt catcaaaagg aagatatccc catttccctt
     4381 ttaggaccca acgaggatgt ggctcgcatg atgcttcggg atgccgccaa tgcccataga
     4441 gtaaccctcc aggagtatcg tgaactcggt ctcaccgggc gaatcgtgtg ggtggatgga
     4501 gctgtcaaag cctatacgtt cgggttccca cgctcccttg atgtgttttg cgtcttgctg
     4561 gaggtggcag atcgatcagt ctacggttta gcccagtatc ttttccgtga attctgtcgc
     4621 gagtttcagg catattcatt cgtgaatacc atggatgatt cagggctacc gagtttagca
     4681 aggacaaaac tggcctatca tccctgtcaa ttagtgccca actacattgc ccagccctcg
     4741 tgagtgatca ggatctcccc cggtggaggg taaaataagt ccgccggcgg cgtggttgtc
     4801 ccttggtcat gcttacgttt tcacctgttc gctccgcttg cgaacaagcg gttacgtcta
     4861 ccggacgtcg caagcaatat tgcggatttc ccttcggcat ggcgcagggc gggaccggat
     4921 ggacgctttg aaccctcctt tgttgttctc gattgctccg tcattaaagg gttcggaggg
     4981 gaacaggtga aaattctgaa tttttcaaca atctcccatg gaaatgcatc gatcgtactt
     5041 gcaggttgtc ctccctgaaa tgacacccgt ataatgccct cttagccgtc catgtgtttg
     5101 ctgtttcatt ccttaatttg gatgagatgt tatgaagggt cttgaattcg gtattgaaca
     5161 attccgggtc accgatgagc cgttctatca agaagtgaac ggggaaattg atctgttcac
     5221 cgtgggtgcc aaaaacaaaa taccagtgat gttgaaaggc cccactggat gcgggaaaac
     5281 ccggtttgtc cagcatatgg ctcatcgact caatcggccg ctgattactg tcgcgtgtca
     5341 tgaagatttg accgcctccg atttgatcgg acgctacctg ttgaaagggg aagaaaccgt
     5401 atgggtggat ggcccgctga ccctgggagt caagcatggc gcccttgtct atctggatga
     5461 agtggttgaa gcccgaaaag ataccaccgt gctgatccat cccttgagtg atgaccgacg
     5521 gtttttgcct atcgagaaaa aagggcagat gattcaggcc gtggatgatt ttttgctggt
     5581 gatctcctat aaccccggat atcaaagtgt cttaaaagaa ttaaagcaaa gcaccaagca
     5641 acgtttcatg gccattgagt ttggttatcc tcccaaagat atcgagacca ggatcgtgga
     5701 gcatgaagca aaggtttcca gggacgtggc catccggtta gtcaaattgg gtgaaaaggt
     5761 acgaaatctc cgtaaccatg gcctggaaga aggtgtcagt acccggctgt tgatttacgc
     5821 agggaccttg atgcaacaag gcgtggcccc cgaccgggct tgcgatgccg ccattacccg
     5881 cccgattacg gacgacaccg atatgcaacg cagcattcag gaactcgtca aagccatttt
     5941 ttagtctgag ctgaccaaca cgcgacaatc cccgcctgcg gacaggccgt gacactcaaa
     6001 aagaaatata gacggcgtgt ttcaggctct ggaagtttga aaaagaccct tctgataata
     6061 ccggccaatc ggatctcttt ttaacaatta tccaggaact tcctcccacc tttcaaacta
     6121 tccccaactt aattcgagaa tatccggtgg tccgctgttt gtcggtttgg gagtaaaatc
     6181 ccttctattt cctgacctga cttgattcga ctgggacccc ctaagtcgct accggcctag
     6241 gcggaaaaat ggccttaacg ctcttcattc caggcgacaa tcaaccgata cggtcaatgg
     6301 attaccgaca caaatcatcc tcagatgacc tcaacagaac cagagatcac cgtatgaagc
     6361 caaaaatgac caaccacccc atgtatactc tgcttcgaga gggaaaaatt actgaattca
     6421 atgcgcggtt caagaccggt gagaaacccg atctgagcaa ttgcgatttc cggagcgtgg
     6481 atctgcgggg cattgaagtg gcaggaatgg atttccgggg aagttatttc cggcaggccg
     6541 atcttcgagg agtagatctt tctcgatgca atttggaagg ggccagcatt cacggcacca
     6601 agatctccgg cacgcttttt cccaaagaac tcagtgctca ggaaattctc ctttcgaacc
     6661 agcagggcac gcgtatgcgc tatggcgtat aaatgctcgt ccttgttctg atccgccttt
     6721 cacacagcat catttcttct acgaccgcaa cccacaaacg aaaaaggcct ccagggccct
     6781 cctccctcgc gaaggctcat tcgttgacac ctttcgctcc tcaacttaca ctgcctttcc
     6841 caagaatcgg taagggaaac ctcatgaatc aaccccagaa cattatcgct atcgtctatg
     6901 acttcgatca tacgctgagt ccgcattaca tgcaggatca cacgattctc cgacacgccg
     6961 aacttgaccc gtccacattt tggaaaagct gcaccgctct gatcgaagcc cgggattacg
     7021 atcaggagct ggcctatatg aaacggatgc tggaagaacc ccgcatcagg atgctttcga
     7081 atcaggacct ccgtgacatg ggcaatcaat tgtccttttt cccgggtgtc ccctcatttt
     7141 ttgaagaact caaccagatt tttaaaaagc ccaaatatga ggaagtgccc atccgcttgg
     7201 aacattatgt ggtcagttcc ggactcaagg ctattttgga gggaagcgtg gttgccaccc
     7261 aggtccgcgc cattttcggc tgtgagtttg atgaagaaga tgggcacatc agtttcccca
     7321 aacgcaccat cagccacaca cagaaaaccc aattcctgtt tcgggtcaac aaaggactga
     7381 cggatttaaa agaagatgtg aacgaccata tgcccgaaga aagccgtcgg gtgccgttcc
     7441 gacaaatgat ttatgtaggc gatggaccga cagacgtccc ctgctttacc gtcatgaaga
     7501 aaaatggcgg gttcgccttg gcggtctata atcccgaaga tcagacccgt cggtcctttg
     7561 aaaaatgtta tcaattgacc ttccacgccg accgtgttca tttcatggct cccgccgatt
     7621 atcggcctgg cagccatctc cgtctcattc tggaaaaaca cattgccgaa gttgccgata
     7681 gaattgtcga tagtcgacgg caagggattg aaggttcccg ggtgcctgct cctctgcctt
     7741 aatcgttcct ccattttctc aacattcttt ccttcccggt cgtaattgtc gcctgattgc
     7801 gcaacacttg tgggtcaagt attgtaaaat aaggaaaatg gactagacag agaaggactt
     7861 cccaccagga ggtggactat gcacattgtc gaaagttttt ttatgaaatt gtttcgtaag
     7921 tctgtcggcg gttcctgttt ccttcgcact gtaacggccc ttgttcttat tggcctgctc
     7981 agcagtatca gttgggcgga cgatccgttt ggcagctcga ccctggctcc caataaaaac
     8041 gatatgatca aagacatggc gtttgaccaa gtcaattgga aacgcccaca aggcgtcttt
     8101 acatggatta ctatggccac gggatacgac acaccttggg attcccttgg acggccagga
     8161 tggatgacga cgggggatgc gtatgttgag ccccttaacc caggcgtttc ggaattcccc
     8221 ttagacacgc agacgtttta catcgttttc gaagcacaac ccttagacgc ccctggccaa
     8281 ttcgcagctg cgtggtttca aatgaatgat gggcaagtga cgtcgaatac gcccgtcgga
     8341 aaagacgtga tcgaactgga aatgaatcag cgatccggat actttcaaat ttcaaagccg
     8401 gcagagggat ggaaacccgg gaagtatcag gtcaagattt tttatggttc accaggccaa
     8461 gcgctgcatg ccgtcaacgt tatcggcacc atggaattta cgatcaaagg gtaaatccta
     8521 gaccctcaga tggtttccac ttattttgtg ggtatccatt tgatagggaa tacctcctcc
     8581 ctccgtttgt aacagattcg tttttacaca taatcaccca tgaagatggg ggatctagcc
     8641 taatcttcct gcaggcctat cggtttcgca caccacaatt gtacagaaga atcaacgcga
     8701 gggattgggt tcgggggcag gagagagcgg ctgggaattg gatgggcggc gggaacgaag
     8761 aatttcttcc agcgtgagca gggcatcacg tccggaattg gtttcaaatc ctttcgataa
     8821 acttgtgtcc gcataggtgt gtccctgttg ctgcgtggcg ctctggctca agctgccaga
     8881 ccagggacgg ggatcttgcg acccatgctt tcgaccccag gccgccagac aagccttggc
     8941 gatgatcttg ttaagcggcg ccggattgta cagcatcttt ttgatgttcc agggcgtcaa
     9001 actgagcggg ttgtagccct tcgataaata cccttccgac aataatcgtt gctccaaatc
     9061 cgtattgggc tggattccta ggaaaaacat caagggaaac acccggtctt ctcccaatat
     9121 cgaagccact attttgtagg actcgatact ttggaggagc gtctcttctg tttcgtccgg
     9181 ggaattcaag gaataattga gaatgacccg cccattgaat cccgcttcct ttagataccg
     9241 gcatccctca tacaaatgtt caagcttgaa gcccatatgc atgctgttca ggaccttttg
     9301 cgagcctgca gtgatcgcga cctctaaatc tcccactccg gattgcacca tcagtttggc
     9361 caactttgga ttaatcaggg aggtccggac gtatcccgac cattcaatat gtaattgttg
     9421 agagatgatc cgttccagga tttccgtaca ttgggggtag gcatccttgc cggtgatgaa
     9481 ttgcgcgtcg gtaaaccaaa accgtctggc cccccattga tggtaatact gagcaatgtc
     9541 cttcaccaca ttatccgccg gtcgatagcg cacccgtttg ccttcaatat agggatagag
     9601 acaaaacgca caatcatacg ggcagccgcg tttgctctgc acgccgatac attcgccgat
     9661 atattcacgg tattgtggaa aaattgacgt gagataagta atatccacgg agagcgcatc
     9721 taacagggcc ggcgtatttt tggtgccctt ggtgaccctt ccctgctctt tgatgatgaa
     9781 ccgttcgtca ccgagaggct cgccattcag gactttgaga attgcgtcct ccccttctcc
     9841 cagaatacca atgatgcctt ccggtaattt ttcaataagc tgatccgcaa aggccgtgaa
     9901 cgctccgccg ccgatcatca ttccgattga gggaaattct ttctgaacca gccagggata
     9961 cgaaaggtta ctccggatat ccgtgtaata gcgatacaga ttctgcaggc cttggaagga
    10021 ggccaccact cgcttcaggg gattgctcgc gtaataaaaa ttaaacgcat gctcgagcga
    10081 ggcatcgcct tcatgcggag aaaaaatctg gatatcccgc catgagaaac acaccaattc
    10141 cggctgaaaa gccttggtca cttcccgtaa ttgtgcttga cgttgatccg gaggaaataa
    10201 ggagagatca agaatctgct gacgcacatc cggccggcgc cggtggatga agtcagccag
    10261 gtaggtaatc ccggtgggat aaaccttttt acaggggaga aagacgtaga ggacagagtt
    10321 catcgtgcta tttcaccgcc acatgaatgg cgacaattcc accggtcaga ttgtgatagt
    10381 ccactgattg aaacccggct tccttcagta attgggcgag ccggttttgg tccgggaaat
    10441 tgcgtattga cgccggtaga tattcataca cccccgtacc atcgcgagcc accttggtgc
    10501 cgatccaggg gagcaaatga aaggagtacc agtcgtaaag ctttcgcagg ccggctgaaa
    10561 ccggacggga aaattccagg cacacaaagc gacccccagg ttgcaatacc cggcagattt
    10621 ccaaaagcgc ttggggaaga ttcccgacgt tgcgaataca aaatccggcc gtcaccgtat
    10681 caaatgtggc agacggaaag gtgagatgct cggcattcat ctgcaaacag gtaatacggt
    10741 tggccaagct ctgggacctg actttatcct ggcccacacg caacatggct aaattcaaat
    10801 ccgctgcgat aatttggctc gtatagtgga gatgttgatc cactaagatc gcgaggtcgc
    10861 aggttcccgc acccaaatcc aacgctcgtg tcccagggat gtcaggaatg taggaaatcg
    10921 ccttgcgttt ccaggaatgg tgcagaccaa aactcaggag cgaattattc aggtcataaa
    10981 atttggcaat ggccgtgaac attcgctgga cggcctgctc ccgagccgga ccggtccatg
    11041 tggacgccgc tttgccaggg atgtcgattt tattcattac aggtaccacc caaaggacct
    11101 agcacccgat cttaagtttt gtcaagaatt aaacccttca gaaaactgga gtgaacgatc
    11161 aaagcctgcc aaaccactgc agggaaaaac cggagaggga attaaataaa tagggtggtt
    11221 tccgcaccgg agcaggcaga aataattgca ggcaagccca ttacttcatc gaccccttga
    11281 gtgaccgctt tggcatcgac tcccatatac tcaagtcccg cgctacaggc gactaatttg
    11341 aactggccga cctgacgggc ttcctcgaac atgtccgaaa tgagcggaac tttgcgttca
    11401 cggatcaatc gggctacttc gggggcttgg gcggcatatt ccggaggaaa atccaattca
    11461 tttaaccggc cttcagccaa ctttttgatg gtccagaaca acagcaccac catcacgtct
    11521 ttccccatcg cggaagccgt taatcccagc gtggccactt gatggagctt atcgtaggtc
    11581 gcattatgtg cataaatgac gaatcgtggc ttaaccccca tggcgcgtca attctccact
    11641 cattactgga agtgttgaaa aatgccgcca gcggcgttct cgccccatgg ccatgctcac
    11701 gtactcctcc gtacgctccg ctcgtccaag cagctgcggc cttgctggac gagccttttt
    11761 gaacactccc tcatagtcct cggggaaatc tctgtcactc atacgcctat gaaaaggcag
    11821 aaaattaaaa taatcaacag ccctcaaggt gaccacccca gattgcggat gtggctcagc
    11881 agcccctgac tcacaactaa cgtttttgac caccatagta ttaccgtctt gtgggacgaa
    11941 ttaagggctg tggtttgcgt cgggtgaggg tccaaccctc ttcctcatat aacaaacagc
    12001 atttcatccg accacagacc ccgatccgat ggctatccat gccattcatg ggtgcgtcag
    12061 cccgaccgcg aggatagacg ggcttaaaat cggttaaaaa gctggagcaa cagagcacca
    12121 atccgcaggt atcaacgcca ctcagtcgag cggcttcctc ccggcttgaa atctggcgca
    12181 tctcaatccg acatccaaat cgcctggcca gatctttgac cagttggcga aaatcaatcc
    12241 gatcatcggc cgtataggtg aaggtgagtg atcgctgggc aaatgaacca aacacttcca
    12301 ctaacgtcat gccaagcccc aattcggcaa tccgttcctg gcaaaattcc tcaccttcct
    12361 gggccaaacg cagctgtcgc gcaatctggc gttcatcttc ttcagtggct tttcgaataa
    12421 tctgccgcat cacccgcatc ggcggggtaa acgggaggaa ttgaggaggg aggtagactt
    12481 tgccaaaggt atggtcattg tctaaatcca acaagaccca atcaccgacc ttcacggtat
    12541 gatcatcggt ccgggttttt ttcacctctc ccagatcccg tactttgacg ccgaccactg
    12601 ccacttcttc tatatattct ctttgatctt cggattcctg caccatgaat cattgcctcc
    12661 ctttactacc aaggaattct gtaggcgtga gggtcaatca taccggactt actggaagag
    12721 gccaagcagg aatcctgttg gctcaaactt gggtactcta acccgttccc gactcacacc
    12781 ggctaacgat gttttcgcag aaagggagga ggggcatgat ccaactggta taaaccgatc
    12841 ggtgtcagtt gatccacgag atgagacacc agggtttccc cgttgggtcc ggctccaaac
    12901 acatcaaacg cctggccgaa atgatagcga cagccaaatg gctccagttc ctgcctcaga
    12961 ctttcaggag gatcccaatt cgcggtgagc aaggccgtac gggccgggtt acgcccactg
    13021 aacataaatg ataaatgatc gggataccaa tccgcacccc ctgtcgcata ggagacaaag
    13081 agtttggctt gagccgttcg acacaactcg gctaaatagg cattggtcaa aaagccgttt
    13141 tctcccacct ccaaccaggt tcccggtgga gagagacagg catatgccgc ataactccgg
    13201 acttctttga gttgttgttg cgaggcaaag accaagggaa tcggaccgta ctttgacaca
    13261 aggttggcca tgaccttgtc ttgcaaagcc gagcggccat cattggtcgg cccgctatcg
    13321 gcatgcacca gcgtgttccg cccccggtct gctaccaaat agcaatttcg cggaagttcc
    13381 acatcacatg gatcttcacc gaaaaacggc acggatacga cctgggcctc ccctattcgc
    13441 caactttctc catgagccaa ctctctgacc tgcgaaaaac ctaactctct cagaagggac
    13501 agataatcat aatgcagcgc tttccggttg cgtcgactcg gcacaaagat cggcacatct
    13561 ttaggtaaat ggtacaaagt gcgaggatcg acatggtcgt catggtcatg ggtgagaaaa
    13621 attccagccg ggcgcggaag gaggtcggcc cacagagacg gcacaggcga ctccgcgaac
    13681 catggcatga gccagggatc aaaccataaa aaattatcct ggtgtcgata catcaaagcc
    13741 gcatgtccca gatgcaccac atcttgatcc cgcgtgactt tacgccaatg tgcctcaagg
    13801 gaggtgttag gagcgatggc cagacaatca tggtctttca ataaggtaaa gagctgcgcc
    13861 aattgccgtt caagatctcg cccgccggcc tgggccaccg tttgaatctc caccatcgta
    13921 tgccggccgg tgagaaaacc aaggaaatgt cccacattcg gcccgattgg ccgctcaaac
    13981 ccgaagggta tcgcttgccg cgtgacggag ttaaaaattc gcaaccctcc atacgtcatc
    14041 ttggccgagg ctgtggggtt tttgggatag tcccagtgaa aggtcccatc ggatttccgt
    14101 ccgcactgaa tatgtcgttg tagggaagga cgagattgca ccacttccgc ataagcatcc
    14161 tccaaacgac gggataattg agggtcatcc acggaagcac gtttggccat aatgtcgctg
    14221 atggcagtct cgataccgct catcatgatc tcatgcacct gatgaagact actgacgacc
    14281 tccgggtcca ccccgcccaa aaacgggaaa ggtccgggcg gttcggtact ct
//
LOCUS       DATIAL010000085         6289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_122211, whole genome shotgun sequence.
ACCESSION   DATIAL010000085 DATIAL010000000
VERSION     DATIAL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122211"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VLA60_02800"
     CDS             complement(<1..279)
                     /locus_tag="VLA60_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08312.1"
                     /translation="MYFLFIQNREGADCSSIEKKKCESNLMMNQKWRRLGARLQTRDA
                     ILLTAICVLMGAIFALDLFTQTGVAIGMLYVSAIMLTPWVPHAKSPFIV"
     gene            619..804
                     /locus_tag="VLA60_02805"
     CDS             619..804
                     /locus_tag="VLA60_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08313.1"
                     /translation="MTTSLEHETVHPKGLGQTRMSRCLFGDWPRRMVLKLALPVTDLP
                     LSLPSCKEHMSTDMKAC"
     gene            828..980
                     /locus_tag="VLA60_02810"
     CDS             828..980
                     /locus_tag="VLA60_02810"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HSF08314.1"
                     /translation="MSTIVLVILILLLVGALPVWPYSSSWGPYPSGGLGLVLVIVLIL
                     ALTGRL"
     gene            1034..1504
                     /locus_tag="VLA60_02815"
     CDS             1034..1504
                     /locus_tag="VLA60_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08315.1"
                     /translation="MPLKKPTWSAIVLASLFLGSMGGMLLASTNPTDSKVSATNVGEP
                     ISTSALHIVNEEGKVRATLSLWDGDHPALIMGDEYCDRRASLAVYGKERTGLTLYGED
                     CKRRVALEVQSDDLPMFVLRDHLDTPRVQVVLNKDGSPLVRLFDANGKTLWEKP"
     gene            1589..3163
                     /locus_tag="VLA60_02820"
     CDS             1589..3163
                     /locus_tag="VLA60_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08316.1"
                     /translation="MFSDSTIRNMTWRVKRMFITGIIVLIASGLFGRVFAADQDKPKS
                     WKAVQGPKELVPDIGPPAVVKDWQRAYPQYQPVGKGDTEEAPDAKPGTPNPPDIHTLG
                     GRTPSSFIFDLSPKDPLEKVIERERQQRSKIKTQQQQLLEERYDLSPQFVEGVTMTRG
                     KPIPKGPTARLPKDLTFEQLANMPPEEIKAKDLFPYLPLPHPLHSTGGMVFPSMMTDI
                     HPERERFDVLHDVPDAFLPEFPPPLFLTSRPDLGDVSQGQEITNENFYALFDGILSPV
                     QFDGVRLLVEKIPQQQFNATDDRKTISPSRGVACFDCHVNGHTNGAIHLNPDNRPQFS
                     RFRIETPSLRGVNIQNVFGSKRALSSVEDFSEFENRSAYFDHDLATAEKKGRRDMTRT
                     EVMKMAQFQKLVDFPPAPKLDLFGRLNSHKASEEELRGETLFFGKAKCVACHTAPYFT
                     DNLMHDLRVERFYHGRAEGSIKTFPLRGIKDSPPYLHDGRLLTLEDTVEFFALVTGVQ
                     LTKEEKADLSAYLRAL"
     gene            3229..3642
                     /locus_tag="VLA60_02825"
     CDS             3229..3642
                     /locus_tag="VLA60_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08317.1"
                     /translation="MKKPAHANTVCIVLLRCLGLMFALAFSLGEEVALGDSFQSTSSD
                     GIQSNGHSNDPSGEDFPLIGGGVRTPPDIGASGTASPTPPVRSESPTDEPQILPDISP
                     VPTHPSTPEKAPVKESEPLGQPNHLQKTPEKIKRF"
     gene            complement(3813..4391)
                     /locus_tag="VLA60_02830"
     CDS             complement(3813..4391)
                     /locus_tag="VLA60_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF08318.1"
                     /translation="MICSFFIAAMMEHTHGQILSVPAVGFDEAITPEPNDPRSVDQTS
                     VLSDVEIQYRIQRRLEVSPYENGEITVHVGQGETVLSGYVEDQDALVDLIEIAYDAGA
                     TNINNQLRIKNHDLPWKKMTDQELKEAVEEELYWSPFVDSVPIRVETHNGIVTLSGRV
                     ENRGEIVDAVENAYEAGAKNVRIRLWIDPTLD"
     gene            complement(4814..5308)
                     /locus_tag="VLA60_02835"
     CDS             complement(4814..5308)
                     /locus_tag="VLA60_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08319.1"
                     /translation="MKIQFNISDEISKEVLALQSTLGMKRVEDLTIHAWKVLRFLIET
                     IRHDRFTLSPEAIHWISQSPRTSPTAETAKELSFTEIDTQILHDIHRLLDDDQLDQAL
                     KATHILKLLKSHTNQGSNSQLVQSEMLLTAAETFAAESDPEKAVQLIHATHQTTKKPS
                     DGDD"
     gene            5534..6148
                     /locus_tag="VLA60_02840"
     CDS             5534..6148
                     /locus_tag="VLA60_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HSF08320.1"
                     /translation="MKPSKDYPVKDFTLKGLDGISDKTLEMHFKLYEGYVKATNSLTK
                     QIGEFLIDGQVDQEEMPAYSELTRRLGFEYNGMVLHELYFENMTSQASKSPQGQDSLL
                     KALKGSFGSFETWKTDFSSVGKMRGVGWAVCNLNPENGRLSNHWISLHEFGNIAGFRP
                     ILVMDVWEHAYLLDFQPSERGEYIEHFLSQVHWDIVTKRLEGRL"
BASE COUNT         1591 a         1468 c         1586 g         1644 t
ORIGIN      
        1 gacgatgaaa ggggattttg catgtgggac ccagggcgta agcattatgg cgctcacata
       61 taacatgcca attgccactc ctgtttgggt gaacaggtcc aaggcaaaaa ttgcacccat
      121 aagaacgcat atggctgtta aaaggatggc atcccttgtt tggagacgag caccgagtct
      181 acgccatttt tgattcatca tcaggttact ttcacacttt ttcttctcaa ttgaggagca
      241 gtccgcgcct tcccggttct gtatgaataa gaaatacatg aactccaaac attaccccga
      301 aggatgagaa tgatgcggct ctccctcggc tgcggttacg tagaagaacg ttgggacctg
      361 tcgtgaaagg gcgtaactcg gaaaagtata gcggcaacga ataagtattg tctaagaggg
      421 aaaaccccaa gatgttaatc cactgtatca ttcagggttg ttgctcaatc aacccagggt
      481 ttttctcagg tcttaccgcc caaacgattc tgtataaata aaaaaatcaa ggatgaagag
      541 gcaaatgaca ttcttgatct cggcatcctg gttttgaacc gatcgccaca tggattcaac
      601 ataaggagag caaaacccat gaccacaagt cttgaacatg aaacggttca tccaaaagga
      661 ttggggcaga cgagaatgtc ccgttgtttg tttggggatt ggccgcggcg aatggtgctc
      721 aagttggctc tgcccgttac agacttaccc ctttccttgc cgtcctgcaa ggaacacatg
      781 agcactgata tgaaagcctg ttaaaaacag aaggaggaac cagatttatg tctacgatcg
      841 tcttagtcat tctcattctt ctgctggtcg gcgccttgcc ggtctggccg tacagttcat
      901 cctggggccc atatcccagt ggcggtctgg gattggtgct ggtgatcgtg ctgattctgg
      961 ctttgacagg aagattataa gccgtgctcc gaaatccttt ttgtacctca tcagaaaaag
     1021 ggagaagttt attatgccct taaaaaagcc cacatggtcg gctatcgtac tcgcttcatt
     1081 gttcttgggt tcgatggggg gaatgctgtt ggcttctaca aaccccactg actcgaaggt
     1141 atcagcaacc aacgtcggtg agcctatttc tacttcagcc ctgcatattg tgaatgagga
     1201 aggtaaggtc cgtgccacgc tgagcctgtg ggacggtgac catccggcac tcatcatggg
     1261 agatgagtac tgtgaccgtc gagcttctct tgccgtgtat gggaaggagc gaacggggtt
     1321 aactctctat ggagaggatt gtaagcggcg ggtggcattg gaagttcaaa gcgacgatct
     1381 ccctatgttc gtgttgcggg atcatttgga tactccccgg gttcaggtcg tgttaaataa
     1441 ggacggaagt ccccttgttc ggctgtttga tgcgaatggg aaaaccctgt gggaaaagcc
     1501 atagcatatt cagaaatgtt ttcatacact tttttccctg aattgaggac catcaatgaa
     1561 cgacagaggg cataaaagaa ggagatcaat gttttccgat tcaacaatta ggaacatgac
     1621 ctggcgggtg aagcgcatgt tcatcacggg aatcatcgta ttgatcgcca gtggtctttt
     1681 tgggagggtg tttgctgcgg accaggacaa gcctaaaagc tggaaggcgg tgcaggggcc
     1741 gaaagagctg gttccggaca ttggtccccc ggctgtggtt aaggactggc agcgggcata
     1801 tccacagtat caacccgtcg gaaaaggcga cacggaagag gctccggacg ctaagccggg
     1861 aacacccaat cctccggata ttcatacatt gggggggcga acccctagtt catttatttt
     1921 tgatctgagt cccaaagatc cacttgagaa ggtgattgag cgggaacgcc agcaacgatc
     1981 aaaaatcaaa acacaacagc aacaattgct cgaggagcgg tatgatttgt cccctcaatt
     2041 tgttgaaggg gtgacgatga ctcgggggaa gcccatcccc aaagggccca cggcccgatt
     2101 accgaaggac cttacttttg aacagcttgc caacatgcct ccggaagaga ttaaggccaa
     2161 agatcttttc ccctacctgc cgttgccgca tccgcttcat tcaacaggcg gcatggtgtt
     2221 tccgtcgatg atgacagaca ttcatcctga gcgtgaacgg tttgacgtac ttcatgatgt
     2281 gccggatgcg tttttgccgg aatttccacc tcctctgttt ttgacaagcc ggccggactt
     2341 aggagatgtc tctcaaggtc aggaaatcac caacgaaaat ttttacgcgt tgttcgatgg
     2401 cattctctcg ccggtgcaat ttgacggcgt tcgattactg gtcgaaaaaa tcccccaaca
     2461 gcaatttaat gcgactgacg accggaaaac gatttcgcct agccgcgggg ttgcctgttt
     2521 tgattgccat gtgaacgggc ataccaatgg cgccattcat ttgaatccgg ataaccggcc
     2581 tcaattctca cggtttcgta ttgaaacgcc gtccttacgc ggtgtcaata ttcagaatgt
     2641 ctttggatcc aagcgagctc tgtcgtctgt tgaggatttt tccgaattcg aaaaccggtc
     2701 tgcctatttc gatcatgatc tggcgacagc cgaaaaaaag ggtcgtcgtg atatgacccg
     2761 aaccgaagtc atgaaaatgg cgcagtttca gaagcttgtc gattttcccc ctgcgccgaa
     2821 gttagacctc tttggccgat tgaactctca taaagccagc gaagaagagt tgcgaggtga
     2881 aacgctcttt tttggtaagg ccaaatgtgt ggcctgccac acggctccct attttactga
     2941 taacctgatg catgacttgc gggtggaacg gttttatcat ggccgggcag agggatctat
     3001 caaaacgttc cccctcagag gaataaaaga ttccccaccc tatctgcatg acggtcgtct
     3061 cttgactctc gaagacacgg tagaattttt tgccttagtc actggagtcc agttgacaaa
     3121 agaagaaaaa gcggatcttt ccgcgtatct tcgagccttg taagctaagt ttttcaagtg
     3181 aaaggttgac gagcccggtg tcgggttccc agaagaggag aatgccctat gaaaaaaccg
     3241 gcccatgcca atacggtctg tatcgtattg ttgcgttgct tgggtttaat gtttgcgctg
     3301 gcattttccc taggggaaga agtggcctta ggggatagtt ttcagagcac atcctccgat
     3361 ggaattcaat ccaatggcca ttcaaacgat ccatccggtg aagatttccc cctcataggg
     3421 ggaggggtcc ggacgcctcc cgacatcgga gcgtcaggta ccgcatcccc cactcctccg
     3481 gttcgatccg aatcacccac agacgaaccc caaattcttc ccgatatctc accggttccc
     3541 acgcatccct cgactccgga aaaggccccg gtcaaagaga gtgagccgtt aggacaaccg
     3601 aaccatcttc aaaagacgcc cgaaaaaata aaacgctttt gagcgttgtc tgtgtgccgg
     3661 gagaaaaggc cgattcgttg ttgaaaattt tgaaaccgta cccccttccc caaggtttca
     3721 cctagaaaac caatgagact caattggttg tctttgtgct gagcatattg aggacctctt
     3781 gtattgagag gaaaggtttt ggcactctca atctaatcta atgtgggatc gatccataaa
     3841 cgaatgcgga cattttttgc tccggcttcg taggcgtttt ccaccgcatc cacaatttcc
     3901 ccgcgatttt cgactcttcc agagagtgtg acgatcccgt tgtgagtttc aacacgaatg
     3961 ggaacggaat ccacaaaggg gctccaatac agctcctctt caaccgcttc tttcaattcc
     4021 tgatcggtca tctttttcca tggaaggtcg tgatttttga tacgaagttg attgttgata
     4081 ttcgtggctc cggcgtcata ggcgatttca atcagatcca ccaaggcatc ctgatcttcg
     4141 acatatcccg agagtaccgt ttcgccctgt cccacgtgaa ccgtgatctc accattctca
     4201 tatggactga cctccaagcg ccgttgaatg cgatattgta tctcgacgtc tgaaagcaca
     4261 gaagtctggt cgacggatcg cggatcattg ggttcaggag taatggcttc gtcaaatcct
     4321 actgcaggga cagacaggat ctggccatgg gtatgttcca tcatggctgc gatgaaaaat
     4381 gaacagatca tacacacgca tacgcatacg aaaaaacgtt tgcatgggga attcataagg
     4441 cacctccaga acgtgtttgc cattctcaga ttggccagga aagctctcac tccgatttat
     4501 tccggtaccc ggctgtggcc ggagagatgg cagagagcag gcaatttctt atcaaaacat
     4561 atcaaaatga gaaaaaacga aacctgggtt aaaccctggg tgttcgagca aagttcaggg
     4621 aaaaggtgga aacgagagga ttgtgtggaa gacccgggct cctctggcgg aaaaaatcaa
     4681 ggaaatcgtt cccttgaagt gcaatcgttc gttcgcaggt gcagaatctt ctcttttttt
     4741 aatgtccagc ggtcaggaaa ttgttcccaa tgccagagtg gtcaatccag gcttcaaaat
     4801 gccagctatg tcttcaatca tctccatcag agggtttttt ggtggtttgg tgggtggcat
     4861 gaattagctg aaccgctttt tcgggatcac tttccgccgc aaatgtttcc gcggcggtaa
     4921 ggagcatctc ggattgtacc aattgactgt ttgaaccctg gtttgtgtga gacttgagga
     4981 gtttgaggat atgggtggct tttaatgcct gatcaagctg atcatcgtca agcagccgat
     5041 ggatgtcgtg aagaatttgt gtatcgattt ccgtaaagga gagctccttc gccgtttcgg
     5101 cggtaggcga ggtccttggc gattgggaaa tccagtgaat cgcctcagga gagagagtga
     5161 aacgatcatg gcgaatggtt tcaataagaa accgtaatac cttccacgca tgaattgtga
     5221 gatcttcaac tcttttcatg ccgagtgtgg attgaagtgc caatacttct ttgcttattt
     5281 catcggatat gttgaattgg attttcatgt ttcataggcc actttttttc tcataaaatc
     5341 aacttcaaca tataacagga tagagctgga aggcgactgg gcaaaaccct ggccgtagac
     5401 cttttaatcc atccttcaaa taggaggttc aagggttttc tccagttacg ctttttctta
     5461 tttcttccta tattgggctc ctagaacaga ttgggagccg atggttggat atatgtgggg
     5521 ggaacttatg cgcatgaagc ccagtaaaga ttatccggtc aaagatttca cgctcaaggg
     5581 attggacggt atttcagata agacgttaga gatgcatttt aagctctatg aaggctacgt
     5641 gaaagccacc aattccctca ccaagcaaat cggggagttt ttgattgacg ggcaggtgga
     5701 tcaggaggaa atgccggcgt attctgaact cacccggcgg ctgggatttg aatataatgg
     5761 gatggttctc catgaattat attttgaaaa tatgacctcc caagcgtcca aatctcccca
     5821 aggacaggat tccctgctga aagctcttaa aggcagtttt gggagttttg agacctggaa
     5881 aaccgatttt tcaagcgtgg ggaagatgcg gggggtcggc tgggctgtat gcaacctcaa
     5941 tccagaaaac ggcaggctct ctaatcattg gattagcctt catgaatttg gcaatattgc
     6001 cgggtttcgt cctatcttag tgatggacgt gtgggaacat gcgtatctgc tggattttca
     6061 accgtcggaa cgaggcgaat atattgaaca ttttctttcc caagttcatt gggatattgt
     6121 gactaaacgt ttagaggggc gattatgatc aacgtcatat gccatggctg gtgcctgcca
     6181 cgtttcgatg ttcgatggac ctctccagtt ccgcaacccg atcctaaccc taacccggac
     6241 cctttcccta atcctggacc tcttccaaac cctatgcctg acccaccgg
//
LOCUS       DATIAL010000086         9889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_122409, whole genome shotgun sequence.
ACCESSION   DATIAL010000086 DATIAL010000000
VERSION     DATIAL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122409"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..744)
                     /locus_tag="VLA60_02845"
     CDS             complement(214..744)
                     /locus_tag="VLA60_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HSF08321.1"
                     /translation="MSVDQWLGLVTGVLFGFFLQKGRVLRFEKQIGAMLLKDMTILKF
                     MLSAILVGMVGITLLTDAGLASFHHKPMNLGAVLLGGALFGAGWAVMGYCPGTSIGAL
                     GEGRWHAVFAVAGMLLGAALYAELYPFFTSTVLAWKDFGQIGLPDALGVSAWIIIPMF
                     WAVVLFLFRWFERKGL"
     gene            complement(741..1400)
                     /locus_tag="VLA60_02850"
     CDS             complement(741..1400)
                     /locus_tag="VLA60_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HSF08322.1"
                     /translation="MRPVLSSHCCSYCLFSHVSRRVVSLQTNDPKCTEEFLMNVKWKT
                     QDGGWSPYLAGGFVGLLAIVSVYATTQLTGKTTYLGASTTFVRAAGMLEQTVVPEHVT
                     VNEYFTKEKVRVDWQFMLVLGIFLGALISSATDRSFKLERLPPTWEERFGSSLTIRAT
                     GALLGGVIAMIGVRMADGCPSGHGLSGMMQLSVSGLVAMAMFFGVGVLVAALVYGRRV
                     S"
     gene            complement(1434..1811)
                     /locus_tag="VLA60_02855"
     CDS             complement(1434..1811)
                     /locus_tag="VLA60_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08323.1"
                     /translation="MKRILLVDDHQMAREALKYFLEMQGYSIEEAENGAVGLALIQKG
                     RSFDLVISDNQMPVMTGLEFIQRLAQQSYISTHPLIMYSGQLTPELEQQVRALGVYAV
                     LQKPYNLHDLLVIVRQAISDSDA"
     gene            complement(2065..3177)
                     /locus_tag="VLA60_02860"
     CDS             complement(2065..3177)
                     /locus_tag="VLA60_02860"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSF08324.1"
                     /translation="MKEACWSFEEYCDDLGPGKIVHLYDPASGMRGIVAIDNIACGPA
                     IGGVRMAVDVSTREVVRLARAMTFKNAAAGLPHGGGKAGILADSRTPDKARVIRAFAC
                     AIRDLVEYIPGPDMGTDETCMGWIHDKIKRAVGLPRVLGGIPLDEIGATGFGLAECAE
                     VAAASCGLTLEGANVAIEGFGNVGKHAALFLQHKGVKLVAASDRRGTMYDPAGIPVAA
                     LVEAKAVSGSVLGYAKGKKLSQQEIFGLPCDILIPAARPDVIHLNNVDGIQAKLILQG
                     ANIPATPEAETRLHQRGILTIPDFIANAGGVICASVEYHGGNEAQAFQAISEKIRRNT
                     EEVLMRSLKENIEPRRAAMDLSRERVLKAMTFRSCE"
     gene            3566..5020
                     /locus_tag="VLA60_02865"
     CDS             3566..5020
                     /locus_tag="VLA60_02865"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HSF08325.1"
                     /translation="MAHCFSTASPFYPKIRFAFTDHPITVWAGAILLRLYFELIGLRA
                     VLQRTLLPMAKRSNHQIPVSDVLLAWFYGLALGAERFAHFTRYRRDPLLPQLLGLPRF
                     PSPDPLRRFFLAFSYARLTTVSETLMRQSLERMPRFLLGHPLDLDSTIFCRYGTQEGS
                     QIGYNPQKRGRPSHHPLIAFLSEARRLLWPTLRAGRAGTANGCVELLTQALTLLPPGH
                     RVALVRADAGFFLTRFLTFLEHQDLPYIIMARLTPVLRRVVLHRLPETAWRRVSTGVE
                     VAEYTATLPSWAGHSRRFVCLRQALRERPHAAGRRLIDCPGYTYQLMVTSVPYAAEIV
                     CRMYAGRADSENRIKELKEDLSLDTFCLQSFDATDAAFRLGCVAYNLLAGFRETVLPR
                     CWVERRLRAVRDFVFLVGADLIPQGRRLQIRFAVPREEREEFLVRLRPLSAGLPIAAQ
                     LEWTLTDEESPSESSQPRPSGAHPAGIPVAQPPP"
     gene            complement(5143..6333)
                     /locus_tag="VLA60_02870"
     CDS             complement(5143..6333)
                     /locus_tag="VLA60_02870"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HSF08326.1"
                     /translation="MAYHSFQQLLQVQLEEIRSAGLFKGEREILGPQGARIRVGQGEV
                     INLCANNYLGLANHPQVIQAAGEGLCHYGFGMASVRFICGTQDQHKRLEEGISRFLGT
                     DDTILYTSCFDANTGLFETILNEEDAIISDALNHASLIDGIRLCKAKRFRYVHSDMQE
                     LESALQKAAGSRVRLIATDGVFSMDGDLAKLDRITELAERYDAVVLVDDSHATGVLGK
                     NGKGTPEHFGVADRVDILTSTLGKALGGGAGGFTSGRREIIALLRQRSRPYLFSNALP
                     PVITAAALKALDIVREGEDLRTTLKKHAQMFRSHLEALGFRLVPGHHPIIPVMLGDAE
                     LATRMAEWLLEEGIYVIGFSFPVVPKGQARIRVQMSAAHTREELERALQAFETVGREL
                     DVIP"
     gene            complement(6394..7428)
                     /gene="tdh"
                     /locus_tag="VLA60_02875"
     CDS             complement(6394..7428)
                     /gene="tdh"
                     /locus_tag="VLA60_02875"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HSF08327.1"
                     /translation="MKALLKTKPEKGFTLMDWPDPQPGPSEVLVRVQATSLCGTDAHI
                     YNWDEWASRRIHLPRIIGHEMFGEVVEVGREVSSVKVGDRVAAESHLTCGVCFQCRTC
                     QAHVCKNYRILGIDFDGSYSEFVALPERVLWKTAPDIPPELGCLHEPLGNAVDAALAE
                     DLTGHTVLVTGCGPTGLFAIAVSRLAGAARIIATDISSYRLQLAKQLGADHVLNAHTE
                     SPEALGASLSDLLGGEGVDAALEMSGDPVALHLAFQTVKNGGRVTLFGIPKGQVCFDL
                     PNEIIFKGIRVYGVTGRRLFGTWYRLAGLFKAGLNIRPVVTHTIPMADFAKGFDLVNA
                     GQCGKVVMVP"
     gene            8454..9395
                     /locus_tag="VLA60_02880"
     CDS             8454..9395
                     /locus_tag="VLA60_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF08328.1"
                     /translation="MRSSWRIPFHLILFTAVLLFWSVVPGQAEPRISILMTQQSGTYQ
                     DVRKGFQRYLDKQGIAVEYDIRNFEGPVQETDAVLQAMKATNPSLILTLGSPATETVI
                     REVKNIPIIAGMIMNEAELTKGENITGIVLEFPLQTHISWIQKLLPQKKTIGALFNPR
                     ENTGTIEQARRIIEDSGLTFYAKKVETPRDLPDALDSLPGNADVLLGLTDSVVFSPET
                     VKGILLFSFRNRIPFVGLSTSWVKAGAVYALDRDYVDIGVQCGELAEQVLKGVNIHTL
                     KPQSPRKVLYALNLNSINHMHLNLSDGVIRGAHPVFE"
     gene            9409..>9889
                     /locus_tag="VLA60_02885"
     CDS             9409..>9889
                     /locus_tag="VLA60_02885"
                     /inference="COORDINATES: protein motif:HMM:NF021499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08329.1"
                     /translation="MMKNLRINLVTKFNTLILSLIIIMALGIGLIVALVQQNHQMEAL
                     HHHGMAVAAMVAQAGEYAMYTENQAALRQVIAQLDTNPDIAYVALRNAQTNILLSKSI
                     NESIQIPMIDLQEKTPAFIRSYNAVNHGDGQTYLEFLAPVVTQPRTDDLFSETEFVPP
                     "
BASE COUNT         2292 a         2894 c         2367 g         2336 t
ORIGIN      
        1 gtctacgaac tctcttgatt gccgcggcca ccaccggatt cattttcgcc ttaagtccgg
       61 aataatcgtc cttccataca ctaaccttcc ccgacctttc acttcacctg aatccaaccg
      121 tttgctctct atggatcaag gacgctgatc ctctatctca ccgggcgttt gctatcggtc
      181 ggtgaaaccg atgcttcgtt cttcactgaa cgattagagt ccttttctct cgaaccaacg
      241 aaataggaaa aggactacgg cccaaaacat cggaatgatg atccaagccg acacacccag
      301 cgcatccggc aggccgatct gtccaaaatc tttccatgcc agaaccgttg atgtgaagaa
      361 gggatagagt tccgcataga gagccgctcc tagcagcatt cccgctaccg cgaagacggc
      421 gtgccagcgc ccttcgccca acgcgccgat ggacgtgccg ggacaatacc ccatcaccgc
      481 ccacccggcc ccgaagagag cccctccaag caggaccgcg ccgagattca tcggcttatg
      541 atggaaagag gccaacccgg catccgtcag caaggttatt ccgaccatgc cgacaagaat
      601 cgcagacagc ataaacttga ggatcgtcat atcctttagc agcatggccc caatttgttt
      661 ttcgaaacgt aacactcgac ctttctgcaa gaaaaatcca aacaacaccc ccgtcacgag
      721 accaagccac tggtcgacac tcatgacacc ctcctcccat agacgagggc ggccaccaat
      781 accccgactc cgaagaacat ggccatggcg accaatccac tgaccgagag ttgcatcatg
      841 ccgctcaaac catgcccgga gggacagccg tcagccattc gcacaccgat catggcgata
      901 actcctccca gaagggcccc cgtggcccgt atcgtcaggg atgacccaaa gcgctcctcc
      961 catgtcggtg ggaggcgctc cagtttaaag cttcggtccg tcgctgagga aatcagcgcc
     1021 ccaaggaaaa tgccgagaac caacatgaat tgccaatcaa cgcgcacctt ttcttttgta
     1081 aaatactcgt tgacggtgac atgctccgga accactgttt gttcaagcat gccggccgca
     1141 cggacgaacg tcgtcgacgc gcccaagtaa gtggtctttc ccgtcaactg agtggtggca
     1201 tacaccgaaa caatggccaa cagaccgacg aatcccccag cgaggtaagg actccatcca
     1261 ccatcctgag ttttccactt cacattcata aggaattcct ccgtgcattt tggatcattt
     1321 gtctggagcg aaacgaccct gcggctgaca tgtgaaaata ggcaatatga acaacaatgg
     1381 gaagaaagga ccggtcttat cccgactcct gacgtaggat cgcgtttttg cgctcaagcg
     1441 tcagaatctg aaatggcctg acgaacgata acgagcagat cgtgtaaatt gtagggtttt
     1501 tggagaaccg cataaacccc taaagcacgg acctgttgtt ctaattccgg agtcagctgg
     1561 ccagagtaca tgatcaaggg atgggtggag atataagatt gctgtgccag tcgttggatg
     1621 aattccagac ccgtcatcac aggcatctga ttgtcggaaa tcacaagatc gaaggacctc
     1681 cctttctgaa tcagggccaa ccctaccgcc ccgttttccg cttcttcaat gctataccct
     1741 tgcatctcaa ggaaatactt taaggcttcc cgggccatct gatgatcatc gaccaggaga
     1801 atacgcttca tcgcttccag ggtccttgct ctctttcgct ttgagagggt aaaggttgtc
     1861 tttgtatatc acatgaaact ctgccaaatc tgggaaatcc atgcgttttg cgtgcgagat
     1921 taatcatatc agctacatcc agaattcgat gctgataaga atcaccccac atcatatgca
     1981 ataccggaga ccatcccgtc agtgaccacc agcccgactc tcccgctcat ccgttgccac
     2041 ggctgtcacc agagatcgcg ttcattattc gcaggatctg aaggtcatcg ctttcaggac
     2101 tcgttcccgg gacagatcca tagctgccct gcggggttca atgttttcct tcagactgcg
     2161 catgagcact tcttccgtat tgcgtcggat tttttctgaa atggcctgaa acgcttgagc
     2221 ctcattcccg ccatggtatt ccacggaggc acagataaca cctccggcat tggcgataaa
     2281 atcaggaata gtcaggatcc cccgttgatg caatctggtt tcggcctcgg gcgtggcagg
     2341 aatattggct ccctgcaaaa tcaatttggc ttgaatgcca tcgacattgt tgaggtgaat
     2401 gacatccgga cgggcggcgg gaataagaat atcgcagggc aaaccaaaaa tttcctgttg
     2461 actcagcttc ttgcctttcg cataacccag aacactaccc gataccgctt tggcttcgac
     2521 cagagcggcc acggggattc ctgccggatc atacatggtt ccccgacgat cactcgcggc
     2581 gacgagtttc acgcctttgt gttgcaggaa gagggcggca tgctttccca cattcccaaa
     2641 gccttcgatg gccacgttag cgccttccag cgtgaggccg catgaggctg ccgccacctc
     2701 cgcgcattcg gcaagcccga accccgtggc cccgatctca tcaagtggta tgcccccgag
     2761 cacccgcgga agtcccacgg cccgtttaat tttgtcatgg atccagccca tgcaggtctc
     2821 gtcggttccc atatccggcc cgggaatata ctccacaaga tcgcgaatgg cgcaggcaaa
     2881 ggcgcgaatc acgcgtgcct tatcgggagt tcgagaatca gccaggattc ccgctttgcc
     2941 ccctccatgc ggcagcccgg cggcggcatt tttaaaagtc atcgctctcg ccagacgaac
     3001 gacttcccgc gtggacacat ccacagccat ccgcaccccg ccgatcgcag gaccacaagc
     3061 gatattgtca atcgccacta ttccccgcat accactggct ggatcataga gatgcacgat
     3121 tttccccggt cccaggtcgt cacaatattc ttcaaaactc caacaggctt ctttcatgct
     3181 ggcactcctt ttcaacgtat caccatcggt tccacatacg ggaaccagtc gaacattcag
     3241 gttctggaat ccatgccctc ggtccttttg acctgctccc aatattgcgg gccgtggcca
     3301 tttggagcaa cacccattca agattattgg tcgaccagat cccgacacgg tctccacgcc
     3361 cgacgttcag cgccatcaac ccttttgcca gtttcgtggc ctcttggtga aacccggcat
     3421 aggagaatcg cctgccttgt ggaatggaaa cgagcgattc agcctgcgga caggtcttaa
     3481 cggtttgttc aacccggatt ccgcagtagg gttttgatca ggtagcatgg gagccatctt
     3541 tcttcctctg ttttaaggag ttccgatggc acactgcttc tcgacggctt caccgttcta
     3601 ccctaagatt cgctttgcct ttaccgacca tcccatcacc gtgtgggccg gagcgatctt
     3661 gctccggctc tatttcgaac tcatcggcct gcgggcggtt ctccaacgga ccctgctccc
     3721 catggcgaaa cgatcgaatc atcagattcc cgtctcggat gtcctgttag cctggttcta
     3781 cggactcgcc ttgggagccg aacgctttgc gcacttcacc cgctatcgcc gcgacccttt
     3841 actccctcaa ctgctgggcc tcccccggtt tccttcccct gatcccctcc ggcgcttttt
     3901 tctggctttc agctatgccc ggctgacgac ggtgtccgaa accttgatgc gtcagagtct
     3961 ggaacgcatg cctcgttttc tgctggggca ccccctggat ctggactcga cgattttctg
     4021 tcgctacggg actcaagaag gcagtcagat tggctacaat ccccagaaac gcgggcggcc
     4081 gtcgcatcat ccgctgatcg cctttctgag tgaagcgcgt cgcctgttgt ggcccacgct
     4141 ccgtgcaggt cgggccggta cggcgaatgg ctgtgtggag ttgctcaccc aggccctcac
     4201 gcttcttccg cccgggcatc gcgtggcctt ggtgcgggcc gatgcgggct tctttctcac
     4261 gcgctttctg acctttctgg aacaccagga tctgccttac atcatcatgg cccgtctgac
     4321 ccccgtcttg cgccgtgtgg tgctccatcg gctgcctgaa acagcctggc gccgtgtcag
     4381 cacgggcgtt gaggtggccg agtatacggc gaccttaccg agttgggccg ggcacagtcg
     4441 ccggtttgtc tgtctccgtc aagcactgcg ggaacgcccg cacgcggcgg gtcggcgcct
     4501 catcgactgt ccgggctata cgtatcagct catggtgacg agtgtgcctt atgccgccga
     4561 aatcgtctgt cgcatgtatg cgggccgggc cgacagtgaa aaccggatca aagaactcaa
     4621 agaggacctc tccctggata ccttttgtct gcagtctttt gatgcgaccg atgccgcgtt
     4681 tcgcttgggt tgtgtcgcct ataatctcct ggcgggattt cgtgagacgg tcttaccgcg
     4741 ctgttgggtg gaacggcgcc tccgggccgt gcgcgatttc gtcttcctcg tcggagccga
     4801 tctgattccg cagggacggc ggcttcagat ccggtttgct gtcccgcggg aagagcgaga
     4861 agagtttcta gtccgcctcc gtcccctttc ggcgggattg ccaattgcgg cgcaattgga
     4921 atggacattg accgacgaag agtcgccgtc tgagtcctcg cagccacgac cgtcgggggc
     4981 tcatccagct ggcatcccag tcgctcaacc cccaccctag gaaatttcta ttgctgaatc
     5041 cgggttcaaa ggcactccag atggtcaccc caagcagagg cgtttctcct ccccgatgaa
     5101 cataggaaca ttgtcgtttg ttggaagtca taccgtcatg ggttatggaa tgacatccaa
     5161 ttcccgtcct acggtctcaa aagcctgaag agcccgttct aactcttccc gggtgtgggc
     5221 ggctgacatt tgcacccgaa tgcgggcctg ccccttgggc actaccggaa aactgaatcc
     5281 gatgacatag ataccctctt caagcaacca ttcggccatc cgtgtcgcca attccgcgtc
     5341 tcccagcatg acaggaataa taggatggtg ccccggaacc aggcgaaagc ctaaagcctc
     5401 cagatggctt cgaaacatct gcgcatgctt cttaagagtt gtccgcaagt cctcaccttc
     5461 cctcacgatg tccaatgctt ttagggctgc agccgtaata acggggggca aggcattgga
     5521 gaacagatac ggtcgcgaac gttgccgcag gagtgcgatg atttcacgcc gcccggaagt
     5581 gaaaccaccg gcacctccac ccagggcctt tcccagcgtg ctggtaagaa tgtccacacg
     5641 atcagccaca ccaaaatgct ccggagtgcc ttttccgttt ttacccaaca ctcctgtggc
     5701 atggctgtca tcaaccagca caacggcatc ataccgctca gccagttcgg taatgcgatc
     5761 gagcttcgcc aaatccccat ccatcgagaa gaccccgtcg gtggcaatca gacgcacccg
     5821 tgagccggca gccttctgca gggctgattc caattcctgc atgtcggaat ggacataccg
     5881 gaaccgcttg gccttacaga gtcgaatgcc atcgatcagg ctcgcatgat ttaatgcatc
     5941 actgatgatg gcatcctcct cattcaaaat cgtttcaaag aggccggtat tggcatcgaa
     6001 gcaggaggta tacaggatgg tgtcatcagt acccaaaaac cgactgatgc cttcctccag
     6061 tcgtttgtgc tgatcctgtg tcccgcaaat aaaccgaacg gaggccatgc caaacccata
     6121 atggcaaagt ccttctcctg cggcctgaat gacctgagga tggttagcca acccgagata
     6181 gttattggcg cacagattga tgacctcacc ctgccctaca cggatgcgtg ctccttgcgg
     6241 tccaaggatt tccctttcgc ctttgaacaa accggcggag cgtatttctt ccagttggac
     6301 ttggaggagt tgttggaagg aatggtaggc catacgaatt tttgaccttt gaacatgtgg
     6361 aatttcatct aatgatgaat ttgctttttg gccttaaggc accatcacaa ccttgccgca
     6421 ttggcctgca ttgacgagat caaacccttt ggcaaagtca gccatcggaa tggtatgggt
     6481 cacgacgggt cgaatgttga gccctgcctt aaacaacccg gccaaccggt accacgtccc
     6541 gaacaaccgg cggcccgtca caccatagac ccgtatgcct ttgaaaataa tttcattggg
     6601 caaatcaaag cacacttgtc ctttggggat gccaaacaag gtcacgcgcc ccccgttttt
     6661 cacagtctga aaggccagat gcaaggctac cggatcaccc gacatttcca aagcggcatc
     6721 cactccctcc cctcctaaga gatcggacaa ggaggcaccc aaggcctcgg gcgattccgt
     6781 gtgggcattg agcacatggt cggcgccaag ctgcttcgct aattgcagtc ggtagctact
     6841 gatatcggtc gcgatgatac gtgcggctcc cgcaaggcgg gagacggcaa tcgcgaacaa
     6901 tccggttggg ccacaaccag tcacaagaac cgtgtgcccg gttagatctt ccgccaaggc
     6961 ggcatcgacc gcattgccta atggttcatg caggcaaccc aactccggtg ggatatcagg
     7021 tgctgttttc cataaaaccc gttcgggaag agcgacgaat tccgaatagg aaccatcgaa
     7081 atcaatgcct aaaatgcgat agtttttaca aacatgcgcc tggcaagttc ggcactgaaa
     7141 gcacacacca caagtaagat gagactccgc agccacccga tccccgactt tgaccgagga
     7201 cacctcacgg cccacctcca ccacttcacc gaacatttca tgcccgataa tcctgggaag
     7261 atgaatacgg cggctcgccc attcatccca gttataaata tgtgcatccg ttccacacaa
     7321 ggacgttgcc tgcacacgca ccaggacttc agaaggacct ggttggggat ccggccagtc
     7381 catgagagtg aatccttttt ccggttttgt tttcagaagg gccttcatac taattttgag
     7441 aatctcactc gttaaggaat gtcgtggttt gctccaagga aaattttttc gaagctacct
     7501 attgaaatac attctccgta aagaacagag agaccttttg gtcttccaga gtccgcactt
     7561 ctcttttgac ttttggcaca ttctcaaatt cgttcgttat agcatgtcct tcctacctgc
     7621 acggcatgtt ggtggaagag taatcgcctc ttcctcttat tctttgggaa ttttttcagg
     7681 agaggcccct ttatgatcta tccctctcac aaaaatctgg gaatttccca cgctttttcc
     7741 aatcaccgct aactgaccat ttgctgctcc cttaataatc ctcaatgcca ccgattcgaa
     7801 atcaataccc cccttttatt atctcataag agatacagca agggtcatgc ctcttcaatg
     7861 agagtctgtg tttttatcag taggaagaaa tatgctgaag atgacccact cccctttggg
     7921 cctttataat taagggtaac ctccataatt tctttgcttt aatctctgct catcattttc
     7981 aattctgcca agttgagtgc ctaaaagggt tattactctc aactctctga ggcattttgc
     8041 cccaactcat ttacctgtga gttcaaccct tccccactcc ctcccatttc tggttgttcc
     8101 ttcgtatccc caactcactg aaaaatataa agaagaaccc atcccaatcc gaaagggatg
     8161 aagatgccct ggcttgattc acagaaaaat ggccgcaacc atttggaatt tcccttctgc
     8221 tgctgcgggg gggggggcgt aagtgaagga gtgccatttt catcaagatt attgtcaatt
     8281 tcctcagtct ctccgctgaa aggagaacaa acgagggggg ttacgccacc accgttcagt
     8341 attatagcgc tccggctcca atgggacatc gccggaatca cattccctta gaataggcgt
     8401 aggtaccgaa tcagcatgac tttggagctt tgaatgacag cgcgagacac tcgatgagat
     8461 cctcctggcg gatacccttt cacctgatct tattcacggc tgtgcttctc ttttggtcag
     8521 ttgtgcccgg ccaagcagaa ccccgaatca gcattctcat gactcagcaa agcggtacct
     8581 accaggatgt ccggaagggt tttcagcggt atttagacaa acagggaatc gccgtggaat
     8641 atgatattcg aaactttgaa ggacctgtcc aggaaacaga tgcggtcctg caagccatga
     8701 aggcgacaaa tccttcgctg atcttgaccc tcgggtcccc tgcgacagaa accgtcattc
     8761 gtgaagtgaa gaacattccg atcattgccg gaatgatcat gaatgaagcc gaactcacaa
     8821 agggtgagaa cataacagga attgtgttgg aatttcccct gcaaacacat atttcttgga
     8881 tccaaaagct gttgcctcag aagaaaacca tcggggcgtt gtttaatccc agggaaaaca
     8941 ccggaaccat tgaacaggcc cgtcgaatta ttgaagactc cggcctgacc ttctatgcca
     9001 aaaaggtgga gactccacgg gacctgcccg atgccctgga cagtctgcct ggcaacgccg
     9061 acgtcctgtt gggtctcacg gattctgtcg tgttttcccc ggaaacagtc aaaggtattt
     9121 tattgttttc cttccggaat cgaattccat ttgtcggcct ttccacgtct tgggtcaaag
     9181 cgggcgccgt gtacgcgttg gatcgggatt acgttgatat tggcgttcaa tgcggagaac
     9241 tggcagagca agtcctcaag ggggtgaata ttcatactct gaagccccaa tcccctcgaa
     9301 aagtgttgta tgccctcaac ctcaactcca tcaaccacat gcacttaaac ctttcagatg
     9361 gagtcattag gggtgctcat cccgtgtttg agtaagcgac caggagatat gatgaaaaat
     9421 ctacggatta acctcgtcac caaattcaat accctgattc tctccttgat tatcattatg
     9481 gccttaggga tcggcctcat tgtggctctc gtccaacaga atcatcaaat ggaggccttg
     9541 catcatcatg gaatggccgt cgccgcaatg gtggcgcagg cgggagagta tgcgatgtac
     9601 accgaaaatc aggcggctct ccgccaggtc attgctcaac tggacacgaa tccagacatc
     9661 gcctatgtgg cgttgcgtaa tgctcagact aacattcttc tatccaaatc catcaatgaa
     9721 tctatccaaa tacccatgat cgatcttcag gaaaaaaccc cggctttcat ccgctcctat
     9781 aatgcggtca atcacggtga cggccaaacc tatttggaat ttctggcccc cgtcgtcacc
     9841 caaccccgaa ccgatgattt attttccgaa accgaattcg tcccacctc
//
LOCUS       DATIAL010000087         5292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_767905, whole genome shotgun sequence.
ACCESSION   DATIAL010000087 DATIAL010000000
VERSION     DATIAL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767905"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VLA60_02890"
     CDS             <1..191
                     /locus_tag="VLA60_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08330.1"
                     /translation="KTMAQLFAYEAIRRLIEQPEQVIGCMLAYRDPGTIQPIPLHAVA
                     PKPFDWELFARMHGTELV"
     gene            415..891
                     /locus_tag="VLA60_02895"
     CDS             415..891
                     /locus_tag="VLA60_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08331.1"
                     /translation="MANLGLFFGFGIFDDAMPQIHAACTYLRQMGFQKIILAGHGLGG
                     AMAIRYGALQPQQFPSPDIQGIIAIATAYSLPGTIRKRWTRFGSQPSFQEVYEKAKTL
                     YDPNSGHDRPDDETIVVKRAHGPTLRPEHSEIYTLKTWWALAGPEADGPKAYQHIV"
     gene            939..1100
                     /locus_tag="VLA60_02900"
     CDS             939..1100
                     /locus_tag="VLA60_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08332.1"
                     /translation="MIDPQECDALGNVVKTAGNPDVTMLHLEAGHDLSGKHEELGQGV
                     VKWIQERFE"
     gene            1150..2043
                     /locus_tag="VLA60_02905"
     CDS             1150..2043
                     /locus_tag="VLA60_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF08333.1"
                     /translation="MIHPHQKLFRLRTRDGFMMSGHLVIKENDLDTNILKTPIVIEVH
                     GLLGNFLARGTPRLLPQALRERDISSFSINTRLAFAGQINGRGIFDETIHDIDAAVDF
                     LTQEGFHHIFILGYSLGASMVLHWAGHRHVPNVKGLILEGTHYAIPDTQRKRLAKWES
                     TPSYEELYAQAKSILGEDPSHSEHDEMVVIYQARGPSRSPLHDEIFTYKTWWHMMGPE
                     AYSAMAYKQISRVTIPMLLLRGENDPLIEAGEAEALKRIAQEEGNTFVSIKEIPRAGH
                     DCMENPEAMLQEILNLLRPSR"
     gene            2547..3965
                     /locus_tag="VLA60_02910"
     CDS             2547..3965
                     /locus_tag="VLA60_02910"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSF08334.1"
                     /translation="MEPNRKLVLLFSIIFLEGYVVLSAELLAIRQTLPFVGSGTDTVS
                     IIIAAILMPLAFGYYAGGQYRGRIRNKLVRNLLIAGAFLTIGLSYLVLTSFFVTLTEG
                     LEWRNRLLLTTFYALLFIITPVYLLGQTVPLVSNYFRRAHLPHAAGKILFYSTIGSFL
                     GAVLTTLFLMNTIGVSGTVTVTVACIAVLIILLSKLRICRSTTAAIFLFLLAAALNSP
                     QALETNGVISNNAYNTAQMEAYGDTLYLRLNGSYASAIYPDKPEYPVFEYVRYIENQY
                     IRYFRHMNMKGKILVLGAGGFTIGLGDTWNDYVFVDIDSDLKELAENQFLKRKLDKNK
                     IFVAVPARGYLNQTKEKFDLIVVDVFHGLNNAPEHLVTKEFFMQVRNRLTPHGIVIVN
                     HLGSPLSEDDYARGLDNTIRSIFPHANRIPLYNFNPWRIVPSDDKNVLYIAQNFSPGG
                     HENIYTDNLNRAAFDKKQEVPN"
     gene            4556..5167
                     /locus_tag="VLA60_02915"
     CDS             4556..5167
                     /locus_tag="VLA60_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08335.1"
                     /translation="MATKKPSKKPAKPTEPEPFNKRKVNPAKFPSDLPLKDLKELFEP
                     VSGWRNVGFFVFPIETLSPAKTVGRGRTNLTLIRPTIVQVDATTPYAGFDRRESPNRD
                     PAVSMHFEPKAYGITAVSTYVMVFTIEVFGQASTFNLSGFAGGGTVANSGSRTLSGQT
                     TVSLVLRNVPPSAQVWGAITQTAGGQWNWYTVQARYPALVVKA"
BASE COUNT         1333 a         1320 c         1356 g         1283 t
ORIGIN      
        1 acaaaaccat ggcccaactc ttcgcctacg aggccatccg gcggttgatc gaacaacctg
       61 aacaggtcat cggctgcatg ttagcctacc gcgatcccgg aaccattcag cccattcccc
      121 tgcatgccgt ggcacccaaa ccctttgatt gggaactgtt cgcgcgcatg catggaaccg
      181 aattggtcta gaaaacgagt gagttccttt ggtaggtgac agggttgaat tcaacgcata
      241 cctgcaactt gggccgttgg ttgagcgggt cagctccaat gacccgcgac ccttacggga
      301 acttcctctt ccctgtcttc tatcgatcgc ttcaatctcc tggatgaaac cgagcatgat
      361 cttccgtaca ttctggcgaa ggatggatat tcctccatta ccatcaatac catcatggcc
      421 aacctgggcc tattttttgg atttggtatt ttcgacgatg caatgcctca aatccatgct
      481 gcgtgtacct atcttcgaca gatgggcttt cagaaaatta ttcttgcagg acatggattg
      541 ggaggagcca tggcgattcg ctatggcgcg ttgcaaccgc aacaattccc atctcctgac
      601 attcaaggaa ttattgccat tgccacagcg tattctttgc ccggcacgat ccggaaacga
      661 tggacacgat tcggcagtca accttccttc caggaagtct atgaaaaggc caaaacactg
      721 tatgatccaa attcagggca cgatcgaccc gatgatgaaa ccattgtcgt caaacgggcg
      781 catggtccaa cactccgacc agagcattca gaaatctata cgttgaaaac ctggtgggct
      841 ttagcaggac ctgaagccga tggtcccaaa gcctaccaac atatagtata gggggtatca
      901 aggttccggt cctgttggtt cacgggaaat atgatgaaat gatcgaccca caggagtgtg
      961 acgcgctggg aaatgtggtc aaaacagcag gcaacccgga tgtgaccatg ctccaccttg
     1021 aagccgggca cgatctttct ggaaagcatg aggaactcgg acaaggggtg gtgaaatgga
     1081 ttcaggaacg atttgaataa tggttgtctt ctacgggact gtccgaaaat ctatttacca
     1141 aagattccga tgatccaccc ccaccaaaaa ttgttccggc ttcgaacacg cgatggtttt
     1201 atgatgagtg ggcatctggt catcaaagaa aatgatcttg atacgaatat tctcaagaca
     1261 ccaattgtca ttgaagtgca tggtctgtta gggaacttcc tggcacgagg aactcctcgc
     1321 cttcttcctc aggcactccg ggagcgggat atttcatcgt tttccattaa caccaggctg
     1381 gcattcgccg ggcaaataaa cggaagagga attttcgatg agacgatcca tgatatcgat
     1441 gcagcagtgg acttcctgac tcaagaagga tttcatcata ttttcatctt aggctatagc
     1501 ctcggtgcca gtatggtcct gcactgggcc gggcatcgcc acgttcccaa tgtcaaaggg
     1561 ctcattcttg aggggacgca ttatgctatc cccgatacac agagaaagcg attggccaaa
     1621 tgggaaagta ctccgagcta tgaggaattg tatgcccagg ccaagtccat tctaggcgag
     1681 gatccctctc acagtgagca tgacgaaatg gtggtgatct accaggcccg tgggccaagc
     1741 cggagcccat tgcacgatga aatctttacc tataaaacgt ggtggcacat gatgggaccg
     1801 gaggcgtatt ctgctatggc ctacaaacag ataagccggg tcacaattcc catgctgctg
     1861 cttcgggggg aaaatgatcc gctcatcgag gcgggggagg cagaagcatt aaaacgcatt
     1921 gcacaagagg aaggaaatac cttcgtgtct atcaaggaaa ttcctcgcgc cggtcatgat
     1981 tgcatggaaa acccggaggc gatgctacaa gaaatcctta atctactcag accctcccgt
     2041 tgactgacct ccttccggta tgggcaagtt aagggcatct gcacaaaaag taagctatac
     2101 aagattcaat agcacttcct cggtgagacc cttggaaggt tactccggtt ctggctcaca
     2161 agtggcgaag cggctggctg gaaagcgtag gaaggactaa ttgtcctggc gaaatgaagg
     2221 cccaaagctc tgattgctga taaaggatag gtgacagcaa caaccgtagg atgacccgaa
     2281 aaaggtgtac atgaaatctg ttatcccgcc gcgttcaaca cgcaaatgcc gatccggtag
     2341 agctacaata taccacacta aaaatagggt cggacgcatg tttggtattc gcaagcacag
     2401 ccgccgtatt accgcatgct atggcaggac cttcgcttcc ttctttgcct ttcgatgtct
     2461 ggctggtata gcgatatgga caaatgactt atagagtaac gttcgaagga ttcttccgat
     2521 tttagcggga gcataataag aagcccatgg aaccaaatcg caagctggta cttttatttt
     2581 ccataatttt tttggaaggc tatgtcgttc tgtccgccga acttctcgcc attcgccaga
     2641 ccttgccctt tgtcggcagc ggcacggata cggtgtccat tattatcgct gccattctga
     2701 tgcccctggc tttcgggtac tatgcgggcg ggcagtaccg gggaagaata cgtaacaaac
     2761 ttgtccgcaa cctgctgata gccggggcgt ttttgactat cgggctttcc tatcttgtct
     2821 tgacatcgtt ttttgtcacg ctgactgaag gacttgaatg gagaaaccgg ttgttgttga
     2881 caacttttta cgcgttgtta tttatcatca cgccggttta cctgctggga cagaccgtgc
     2941 cgctcgtcag caattatttc cgtcgggcgc atttgccgca cgctgccgga aaaattttgt
     3001 tttactcgac gatcggctcg tttctgggtg cagttttgac cacccttttc ctgatgaata
     3061 cgatcggcgt atcgggcacc gtcacagtga ccgtggcctg cattgctgtt ctgatcatcc
     3121 tgctatcaaa gctcaggatc tgtcggagca cgactgcggc aattttcctg ttcctcctcg
     3181 ccgctgccct taacagtcct caggcgcttg aaacgaacgg ggttatttca aataacgcgt
     3241 ataataccgc gcagatggaa gcctacggcg atacccttta cctgcgtctg aatggatcgt
     3301 atgcctcggc tatttatccg gataaaccgg aataccctgt ttttgaatat gtgcgttata
     3361 tagagaatca gtatatccgg tatttcaggc acatgaatat gaaggggaaa attctcgtcc
     3421 tcggcgcggg cggctttacc atcggtcttg gcgacacatg gaacgattat gtttttgttg
     3481 atatagactc tgatctgaag gagttagcgg aaaaccaatt tctcaaaaga aaactggata
     3541 aaaacaaaat ttttgttgcc gtaccggcca ggggatatct taaccagacg aaagaaaaat
     3601 tcgatctgat tgtcgtcgat gtgttccacg ggctgaacaa tgcgccggag catctggtaa
     3661 caaaggagtt ttttatgcag gtgaggaaca ggctgacacc gcacggcatt gttatcgtca
     3721 atcacctggg ctccccgctc agcgaggacg attatgcgcg tgggctagac aacacgatcc
     3781 gtagcatctt cccccatgcc aaccgcatcc ctctatacaa tttcaatcca tggcgaattg
     3841 ttccgtccga tgataagaac gttctgtaca tcgcacagaa tttttcgccg ggcggtcacg
     3901 aaaatattta cacagataat ttgaacagag cagccttcga caaaaagcag gaagtgccga
     3961 attaactgcc gctgtggcct cggaagttgg cagtcgtctt cccccgtgtg ctgacagtct
     4021 ccgaatagtt tccggctgtc tggttgacgc cgactccaaa cgtcccgccg atataataat
     4081 aaaatgaacg cagcctcttt acagatagaa tcgtccaacc aggtccgtcg gatgccgagc
     4141 cgggatgttc ggattatgcc aggggagata ctcttggcct attggaggat tatgaaccca
     4201 ttgaattttc aatcgacgcc aacaactcct gattttggta gaaggtcgga accctcaagc
     4261 ggagagaaca ttgccggtgg aatttcttaa cacttcctga agtggaaatt gtatattagg
     4321 gaacatgcca ttctgaatca tggcatgagt gagagggcca taggaatttt gcaatcatca
     4381 gcgcctgatt gcctagtgag acggattgtt gatttttgga gaaattgtca cctcaagcgc
     4441 aattcctccc gttagagggg gagttgattt ttaagccgaa aagagtagag tagcgcaatc
     4501 gcccggtccg gaaatgggag gcgaacggac ttcacccacc acaaagagag tcattatggc
     4561 aacgaaaaaa ccatccaaga aacctgcgaa gccgaccgag cctgaaccat tcaacaaacg
     4621 caaggtgaat cccgccaaat ttccatcaga tctgccattg aaagatctaa aggagttgtt
     4681 cgaaccggtc agcggctggc gtaatgtcgg cttttttgtt tttccaatag agaccttgag
     4741 ccccgccaag accgtcggtc gtggccgcac aaatctgacg ctcattaggc cgaccattgt
     4801 tcaggtggat gcgaccacgc cgtatgccgg attcgatcgt cgggagtcgc ccaatcggga
     4861 tcccgccgtg tccatgcact ttgagccgaa agcctacggg attacagccg tctcgactta
     4921 cgtcatggtg ttcacgatcg aggtctttgg acaggcatcg acgttcaacc tatcgggatt
     4981 tgccggaggc ggcacggtgg cgaacagcgg ctcgcggacg ctcagcggcc agacgacggt
     5041 gtcgctggtg ctcaggaacg tgccgccatc ggcacaggtc tggggagcca tcacgcagac
     5101 ggcgggcggg caatggaact ggtacaccgt gcaggctcgc tatccggcgc tggtcgtgaa
     5161 agcgtaaacg ctcgcagtcc ccgtgagtag aggcttcacg agctgagcag gtcgctgcac
     5221 gaaatttcct ggactgtgga aggcttgagg ggatatttcc gagcgatagc tatggaatga
     5281 tccaggacga tc
//
LOCUS       DATIAL010000088         6956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_552672, whole genome shotgun sequence.
ACCESSION   DATIAL010000088 DATIAL010000000
VERSION     DATIAL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552672"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VLA60_02920"
     CDS             <1..1347
                     /locus_tag="VLA60_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSF08336.1"
                     /translation="VSTWAFSADQSGPSGKILRKVHTFTPHARSVTRLSVSQRDKGFL
                     TADEQGTVLLHYSTTGETILEIPGRGKAIESLRYAPKADGVVWLEEGGTLQTYAIDNP
                     HPEVTFQSLFFPVTYEGYDRPEMIWQSSSGSDEFEPKLGLIPLMFGTLKGTLYAMVLA
                     IPLAVMGAIYTGMFMHPHLRAVVKPSLELMAALPSVVLGFLAGLWFAPLLEKIFPAVV
                     AIVGLLPLVIAVCCLAWQFLPIRVKRFEAYGLDLMVMMVAIVLTVSACLMANQSIEGW
                     LFGGNFKQWLIQNFGLTYDQRNAIVISFAMGFAVIPIIFSIAEDAIANVPRQLVAGSL
                     ALGATRWQTLTRLVLISASPGIFSALMIGFGRAIGETMIVLMATGNTPIMDWSMFNGF
                     RTLSANIAVEMPEAPHGGTLYRVLFLSGLILFGFTFTINTIAEIVRQRLRQKYSQF"
     gene            1359..2990
                     /gene="pstA"
                     /locus_tag="VLA60_02925"
     CDS             1359..2990
                     /gene="pstA"
                     /locus_tag="VLA60_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSF08337.1"
                     /translation="MKFLQRFFASGTLFIWLAGGALACSLLLIGGLLTLIFINGMGYF
                     WPKDLVQLTLKDDTRVLGEIVDTQRIPETVTADFPDGQSRIQLKVGNRDVYGLDFQWV
                     DEADIAQRTWPQDVIAFERREWGNFYGWVTEVWDGETRLAQGRNIGWDVWYPFLEEAE
                     GLHQKIHHIERDVIGAINADMERARLNLKVLESKGEISQDSADILQREIQQREAAYQE
                     QIERLVGLYERLNRYRVTLVDVTGEEKTFPVGGLVKAWRPNAMSVWDKTLMYVKNFWN
                     VLTDEPREANTEGGIFPAIFGTVMMVLVMSVVVVPFGVLAAVYLKEYAKQGLLTRMIR
                     IAVNNLAGVPSIVFGVFGLGFFVYAVGGTIDQLLFSASLPNPTFGTGGMLWASLTLAL
                     MTVPVVIVATEEGLSAVPREYREGSLGLGATKFESLIRVVLPCAMPGILTGLILAVSR
                     ATGEVAPLMLTGVVKLAPALPVDNQFPFLHLDRKFMHLGFHIYDVGFQSPNVEAAKPM
                     VYVTTLVLVLVVLLLNMTAIALRTRMRKRYAGASV"
     gene            3004..3912
                     /gene="pstB"
                     /locus_tag="VLA60_02930"
     CDS             3004..3912
                     /gene="pstB"
                     /locus_tag="VLA60_02930"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HSF08338.1"
                     /translation="MEMEQNFLEQSIPLVKTSGAKPLRLVVETRQTDPMKKTVVESEM
                     AQHSSTDGPAKIVIQDMNFYYGNVQALKCVNMTVPANKVTAFIGPSGCGKSTLLRCLN
                     RLNDLVDGARVEGTILLDEENIYRPEVDATDLRTRVGMVFQKINPFPKTVWQNVAYGP
                     QLQKIRKRAILDEIVETSLQGAGLWDEVKDRLHASALGLSGGQQQRLCIARALAVNPE
                     VLLMDEPCSALDPISTGRIEELLHTLKERLTVVIVTHNMQQAARVSDLTGFFLLGELI
                     EFDDTKKIFTNPGDSRTEDYVTGRFG"
     gene            3942..4601
                     /gene="phoU"
                     /locus_tag="VLA60_02935"
     CDS             3942..4601
                     /gene="phoU"
                     /locus_tag="VLA60_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HSF08339.1"
                     /translation="MKRHFDDSLADLRQRILRMGALVEGQIRQALTALVDRDDVVANQ
                     VIQNDRQVNTMDVVIDELCLELLALHQPAAKDLRFVTTAMKISTELERMSDLAENICE
                     RAIELHAEPQLKPYIDIPRMAERAIKMVGEALDAFVRGDSVLARQVLNEDDYVDELNE
                     QIFRELLSFMMENPQTISRAIRLSFISKYIERIADHATNVAELVVYMVEGKIIRHMNP
                     T"
     gene            4787..5854
                     /locus_tag="VLA60_02940"
     CDS             4787..5854
                     /locus_tag="VLA60_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF08340.1"
                     /translation="MQKWIDTRGGSSRYFQGQVTPLRSQGPGSLWSLVLAGGHGERLR
                     EYTEQRFGAYRPKQYCAFMGKRSMIQHTWRRAKALCLPHRVVTVMDQSHWGYVRTQLI
                     QESLGRVVWQPDNRGTGPGVFLPLSYIRAHDPHATVVLWPSDHFIFPVEPMIQVVRDA
                     AALVQEQPHRMVLLTVPPQSREQDYGWVIPGGRLPHPGGIQVREVRRFVEKPQGSQLE
                     DIALSGGQWNTLVLIANVQTLWEAGRQCIPEVVLPLEEHAGGIGTSSEHQQLLHLYRT
                     MPTRNFSSDLLSLIPQQLHMMDLTGVCWSDWGRPERIQETIACMRQPELRGIGSESVG
                     RVWMTSCQKETVQEEFNGVMG"
     gene            complement(6288..>6956)
                     /locus_tag="VLA60_02945"
     CDS             complement(6288..>6956)
                     /locus_tag="VLA60_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HSF08341.1"
                     /translation="SLFTCHPVFGEDVTDLFNALTGYSKKDSYAKLLIAPVGIRTNII
                     ERINREISRQQQHGDGYLAFKVNSLVDKASIQALYRASQAGVKIELMVRGMCGLRPGI
                     PGLSQTITVTSLVGRFLEHSRCFYFRNGGQDELFLGSADLMPRNMDRRVEILFPIENP
                     TLRTFIVETVLRPYLKDNMHTRQLQSDGTYTRVKPLEHEAPFQAQDWFIANWKGKYTK
                     PPSF"
BASE COUNT         1585 a         1548 c         1985 g         1838 t
ORIGIN      
        1 gtgagtacat gggcgttctc agccgatcag tccgggccgt ccggaaaaat cttgagaaaa
       61 gtccatacct tcactcctca tgcccggtcg gtgacaaggc tctccgtgtc gcaacgggat
      121 aaaggatttc tcacggccga cgagcaagga accgtgttgc tgcattattc cacgactgga
      181 gaaacgattt tagaaattcc tggtaggggc aaagccatcg aatcgctacg ctatgctcca
      241 aaagccgatg gcgtggtgtg gttggaggaa ggtggcacgc ttcagaccta tgctattgat
      301 aatccccatc cggaggtgac gttccaatcc ttattttttc cggtgaccta tgaaggctat
      361 gaccgtccgg agatgatttg gcaatcctcc agtggatcgg atgagtttga gccgaaattg
      421 ggacttatcc cactcatgtt tgggacgttg aaggggacct tgtatgccat ggtgttggcc
      481 atacctcttg cggttatggg agccatttat acggggatgt tcatgcatcc ccatcttcga
      541 gctgtggtga agccgtctct ggaattaatg gcggctctac catcggtggt attgggcttt
      601 ctcgcgggcc tctggtttgc tcctctctta gaaaagatct tccctgcggt ggtggccatt
      661 gtgggattgc tgcctctggt gatcgccgtc tgttgtctgg cgtggcaatt tcttcctatt
      721 cgggtaaagc gctttgaggc gtatggcctt gatctgatgg tcatgatggt tgccatcgtt
      781 ctcacggtga gtgcctgttt gatggccaat cagtcgattg agggctggtt gtttggagga
      841 aacttcaaac aatggttaat acaaaatttt ggtttaacgt atgatcagcg taatgccatt
      901 gtcattagtt ttgcgatggg ctttgcggtg attccgatta ttttcagtat tgctgaagat
      961 gccattgcta atgtgccgag gcaacttgtc gccggatctc tggccctggg ggcgacgcgt
     1021 tggcagacct tgacccggtt ggtcctcatt tcggccagtc cggggatctt ctcggcattg
     1081 atgatcgggt ttgggcgtgc gataggcgaa acgatgattg tgttgatggc caccggaaat
     1141 actccgatca tggactggag catgttcaat ggatttcgta cgctatcggc caacattgcc
     1201 gtggaaatgc ctgaagctcc tcatggcggg accctgtatc gtgtgttgtt tctgtccggt
     1261 ctgattttgt ttggattcac ctttacgatt aatacgattg ccgaaatcgt ccggcaacgg
     1321 ctccgccaaa aatattctca gttttaagtc gagcagccat gaagtttctt caacggtttt
     1381 ttgcatccgg cacattattt atctggttgg caggcggtgc cttagcctgt tctctgctcc
     1441 tgatcggcgg tctcctgacg cttattttta ttaatggcat gggttatttt tggcccaagg
     1501 atcttgtgca attgacgctc aaggatgaca cccgggtgct cggggaaatc gtggataccc
     1561 agcgcattcc ggaaacggtc acagcagatt ttcccgatgg gcaatctcgc atccagctga
     1621 aggtaggcaa ccgggatgtg tacggactgg atttccagtg ggttgatgaa gccgacattg
     1681 cccagcggac ctggcctcag gatgtcatcg cttttgaacg acgggaatgg gggaattttt
     1741 atggctgggt cacggaggtg tgggatgggg agacgcgcct ggctcagggc cggaatattg
     1801 ggtgggacgt ctggtatccc ttcctggagg aggccgaagg tctccaccag aaaattcatc
     1861 atattgaacg cgatgtgatt ggcgctataa acgctgacat ggagcgggcg cgcctgaatc
     1921 tgaaggtttt ggaatcaaaa ggggaaattt ctcaggattc agccgacatt cttcaacggg
     1981 agattcagca acgggaagcg gcctatcagg agcagatcga acgattggtg gggttgtatg
     2041 agcgattgaa ccggtatcgc gtcaccctcg ttgatgtcac cggtgaggaa aaaacatttc
     2101 cggttggcgg ccttgtaaag gcctggcgtc cgaatgccat gagtgtatgg gacaagaccc
     2161 tgatgtacgt gaaaaatttc tggaatgtct taacagatga gccacgggaa gcgaataccg
     2221 aaggtgggat ctttccggca atttttggta cggtcatgat ggtcttggtg atgagtgtgg
     2281 tggtggtgcc gttcggagtg ttggccgccg tgtatttaaa agaatatgcc aagcagggcc
     2341 tgttgactcg aatgatccgg attgcggtga ataatcttgc gggggttccg tcaattgtct
     2401 ttggtgtgtt cggcctgggg ttttttgtct atgcggtggg aggaacgatt gatcaattac
     2461 ttttttccgc ctcgctgccg aatccgacgt ttgggacagg gggaatgtta tgggcttcgc
     2521 tcacgttggc cctcatgacc gtgccggtgg ttattgtggc gacggaggaa ggcttgtcgg
     2581 cggttcccag agagtatcgg gaagggtcgt tggggttggg cgcgacaaaa ttcgaatcgc
     2641 tcatccgggt tgtgctgcct tgcgccatgc cggggatatt aaccgggttg attctcgcgg
     2701 tgtcccgggc tacgggggaa gtggcgccac tcatgttaac cggcgtggtc aaacttgctc
     2761 cggctctgcc ggttgataat cagtttcctt ttctgcatct tgatcgaaaa tttatgcatt
     2821 taggatttca catttacgat gtgggatttc aatcccctaa tgtggaggcg gccaaaccca
     2881 tggtctacgt cacgaccctg gttctggttc tggtcgtcct tcttttgaac atgacggcaa
     2941 ttgcgcttcg aacccggatg cggaagcgct atgctggtgc ttcggtctaa atgaggtaca
     3001 attatggaaa tggagcagaa ttttctggaa caatctattc ctctggtcaa gacctccggg
     3061 gcaaagcctc taaggctggt ggttgaaaca aggcagacgg atcccatgaa gaaaacggtt
     3121 gtagaatccg aaatggctca acattcatct acggatggac ctgcgaaaat cgtcattcaa
     3181 gatatgaact tttactacgg aaatgttcag gctctcaaat gtgtgaatat gaccgttccc
     3241 gccaataaag tgacagcatt tatcggacct tcaggttgtg gaaaatcgac tctcttgcgc
     3301 tgtctcaatc gtctcaatga tctggtcgat ggtgccaggg tcgaaggaac cattctcctg
     3361 gatgaggaaa atatttatcg acctgaagtc gatgcgaccg atcttcgaac ccgggtgggc
     3421 atggtgtttc agaagattaa tccgtttccg aaaacggtct ggcagaatgt ggcctatggc
     3481 ccccaattgc agaagatccg caagcgtgcc attcttgacg aaatcgttga aacaagtctt
     3541 cagggagcag gattgtggga tgaggttaag gatcggttgc atgccagcgc cttgggcttg
     3601 tcgggaggtc aacaacaacg actctgtatt gcgagggcac ttgccgtgaa tccagaagtg
     3661 ttgttgatgg atgaaccgtg ttccgcactg gatccgatct ccaccgggag aattgaagag
     3721 cttcttcata cgttaaaaga gcggctcaca gtcgtcattg tgacacataa catgcagcag
     3781 gctgcccgag tctctgattt gacgggattt ttcctcttag gggaattaat cgaattcgat
     3841 gacaccaaga aaattttcac caatccaggc gattcccgaa cggaagatta tgtgacgggg
     3901 cggttcggct gatttccgat agaccagaat aaaggatcac gatgaaacga cattttgatg
     3961 attcgttagc ggatctcagg cagagaattt tgcgcatggg agcgctcgtt gaggggcaaa
     4021 ttcgtcaagc ccttaccgca cttgtggatc gtgatgatgt cgtggctaat caggtcattc
     4081 agaatgatcg tcaggtgaat acgatggatg tggttatcga cgaactttgt ctcgaattgt
     4141 tggcacttca ccaacctgcg gcgaaagacc ttcggtttgt cacgaccgcc atgaaaattt
     4201 ccaccgaatt ggagcgtatg agcgatttgg ccgaaaatat ctgtgaacgt gccatcgaac
     4261 tgcatgccga acctcaattg aagccgtata ttgatattcc gcgtatggct gagcgtgcca
     4321 tcaaaatggt gggagaagcc ctggatgcgt ttgtccgagg ggactcggtc cttgccagac
     4381 aggtcctcaa cgaggatgac tacgtggatg agctgaatga acaaatattt cgcgaactct
     4441 tgtcttttat gatggaaaat ccccaaacaa tttcgcgtgc gatccggttg tcgtttatct
     4501 ccaaatacat tgaacgcatc gccgatcatg cgaccaatgt ggcggaactg gttgtgtata
     4561 tggttgaagg gaaaattatc cgtcatatga atccaacctg acacttcgtc tccctgcctg
     4621 ctgttttctc cttgtcattt tctcctgttg gggtgttccc gctccagcct cttcgcgtct
     4681 gtcacaaaaa atttacctgt ccgtgacagg ggtgtcattt ctcaaacgta tagtgtgggt
     4741 atgaagaatc atcaacatac accagcgcat gcggaaggag aatgatatgc aaaaatggat
     4801 tgacacccgc ggaggttcct cgcggtattt ccaaggtcag gttacgccgt tacgttcaca
     4861 gggccctggt tcgctgtggt ctctggtcct ggcaggtgga catggggagc gccttcggga
     4921 atataccgaa cagcggttcg gtgcgtatcg accgaagcaa tattgtgcgt ttatggggaa
     4981 acgatcgatg atccaacata cgtggaggcg cgcgaaagcc ctttgtcttc ctcaccgggt
     5041 tgtgacggtg atggaccaat cacactgggg ctatgtacgg acacaattga tccaggagtc
     5101 ccttggacgg gtggtctggc aacctgataa tcgaggaacc ggacccggtg tgtttctgcc
     5161 gctctcctat attcgagcgc atgatcctca tgccacggtg gttctgtggc cttctgatca
     5221 tttcattttc cccgttgagc cgatgattca agtggtgcgt gatgctgccg ccctggtaca
     5281 ggaacaaccg catcgtatgg tgttgttgac tgttcctcct caatcgcggg agcaggacta
     5341 tggatgggtg attccaggtg gtcgtctccc gcatccggga gggattcagg tcagggaagt
     5401 gcgaagattt gtggagaaac cacaggggtc tcagcttgag gacattgccc tgagtggagg
     5461 gcaatggaat acattggtgt tgattgccaa tgtccagacc ttatgggaag ccggtcgtca
     5521 atgtatcccc gaagtcgtgc tgcctctaga agaacatgcg ggcgggattg ggacctcaag
     5581 cgaacatcaa caattactac acctctatcg aacgatgccc acgagaaatt tctcgagcga
     5641 tctcttatcg ctgattcctc agcaactaca catgatggat ctgaccgggg tctgttggag
     5701 tgattgggga aggcctgagc gcattcagga gaccatcgcg tgtatgagac aacccgaact
     5761 ccgtggaatt ggtagtgagt cggttggccg ggtttggatg acgtcctgtc agaaggaaac
     5821 ggttcaggaa gaattcaacg gtgtcatggg atagagaatc tgatgattca atgcgatgta
     5881 tgtgtcggct ggatggttga ctaggaggtt ttcggtcgaa aaatattctg atggcccaga
     5941 ttaggatgaa gtgccagccc cggttatcgg ggcttggttg ataaagatgt tccaagaaaa
     6001 gaggagggaa tgtatgagga gagttagaat ggaaggtgag ggagactgtg ccccaaatgc
     6061 ttcgtttcag gaccgatccg gtacgatcca attgcggtgc cgtagttgtg gtctggagtg
     6121 ggagcccgct tattatcaag agggatgtcc gctgatggag ttggaaacag tatttctttc
     6181 ggaaaattgg tttttagtgt ctagcgaagc caccaggtaa ttttggcaga ggcgcggaga
     6241 tatgttggca tagcgaaaag aaatatacga cttctcattc accgtcttca gaaggaaggc
     6301 ggcttggtgt acttcccttt ccagtttgca ataaaccaat cctgggcctg aaatggtgct
     6361 tcatgctcca gaggcttgac gcgagtatag gttccatcgg attggagttg tcgcgtgtgc
     6421 atgttgtctt tgaggtaggg acgtaagacg gtctcaacaa tgaaggttcg gagtgtggga
     6481 ttctcaatcg gaaacaaaat ttcaacccgc cggtccatat tgcggggcat gagatccgca
     6541 cttcccaaaa ataattcatc ctgccctccg ttacgaaaat aaaaacagcg ggaatgctcc
     6601 aagaaccgtc cgacgagaga cgtgaccgtg atagtttggc tgagcccagg aatcccaggg
     6661 cggagcccgc acatcccacg gaccatgagt tcaattttga cccctgcttg agaggctcga
     6721 tacaaagcct gaattgaagc tttgtcgacg agagaattta ccttgaatgc caggtagccg
     6781 tctccatgtt gttgttggcg ggatatttcc cgattgattc gctcgataat attcgttcga
     6841 atcccgaccg gcgcaatcaa aagtttcgcg taggagtcct ttttggaata cccggtcaag
     6901 gcattaaaca gatcggtgac atcttcgccg aataccggat gacaggtaaa taaact
//
LOCUS       DATIAL010000089         7476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_768101, whole genome shotgun sequence.
ACCESSION   DATIAL010000089 DATIAL010000000
VERSION     DATIAL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768101"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VLA60_02950"
     CDS             <1..354
                     /locus_tag="VLA60_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSF08342.1"
                     /translation="HRVKSLKNPDKRVKELVGYVKSLNMATPHLDFALEVEKITAVKK
                     DNLILNVDGTMAAVLVDIGFPVDSLNGFFILSRTIGMIGHWTDQKRQGSRLIRLFDYL
                     VNYASPKRREVPPLK"
     gene            431..2677
                     /locus_tag="VLA60_02955"
     CDS             431..2677
                     /locus_tag="VLA60_02955"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HSF08343.1"
                     /translation="MSLEMVQKLYASMPGIVDKARKKFGRPLTLTEKILVSHADDFDT
                     QVWERGKAMLALRPDRVAMQDATAQMAMLQFMQAGKDKVAVPSTIHCDHLIRAEVGSE
                     KDLLRACDENREVYNFLASAAKKYGIGFWKPGAGIIHQVVLENYAFPGGLIIGTDSHT
                     PNGGGLGMLAIGVGGADAGEVMAGLPWEVLHPKLIGVKLTGKLNGWASPKDVILYICG
                     LLTVKGGTNKIVEYFGPGAETISATGKGTITNMGAELGATTSIFPFDEKMVAYLNITE
                     RADIAKLAAANRSMLVADPEVYQSPEKYFDEIVEIDLSTLEPHVVGPHTPDLARPISK
                     MAAEAKEKGYPVQLKATLVGSCTNSSYEDIGRAAHIAKQGLQAGLKAKTAFLVSPGSE
                     RIYHTMKREGFLETFESMGATVLANACGPCIGQWKRADAVKGKADSIVSTFNRNFPGR
                     NDGVSETLSFLASPEVVTAYAFSGDLGFDPIKGTLKTADGKDFKFEAPQGDELPAKGF
                     AKGEEGLVQPAEDGSKLQVDIPPTSERLQLLKPFPKWDGKDFEKLPLLIKTKGKTTTD
                     HISPAGPWLKFRGHLDKISDNMFLGANNAFADEAGKANDVLTGESGLTPAQVARRYKE
                     KGLGSFVVGDENYGEGSSREHAAMSPRFLGVLAVLTKSFARIHETNLKKQGILALTFA
                     DPKDYDQIEQEDRISVKGLSGLAPGKPVQVVLHKKNGQEVVIQANHSMTEQQITWFKA
                     GSALNALN"
     gene            2815..3471
                     /locus_tag="VLA60_02960"
     CDS             2815..3471
                     /locus_tag="VLA60_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSF08344.1"
                     /translation="MVTVEIEGKPFQVPAGITLIKALWYAGQDVIRGVGCLGGFCGAC
                     ATYYRTKDDPKVKTCLACSMAVEDGMSFSFMPAFPARKATYNLLELKDPKQDLFTLYP
                     EAPLCRNCNACTEACPQQIDVREGVWKAVFGDFKAVSEMFMDCVMCGMCAPVCIADIA
                     PNLVALYASRAQGVHFTEKPEGLSKRIQEITDGHFQQEWDRILKLSDEELQNTSAATT
                     "
     gene            3493..5070
                     /locus_tag="VLA60_02965"
     CDS             3493..5070
                     /locus_tag="VLA60_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF08345.1"
                     /translation="MELTGQRNLVHQTRDARRSKSFPKLSPAERDSLIKKYHPDHRAH
                     AYRPIIFGPNAGESTVTEVATLLEGESPVPADLNLTPDYSVDVLVVGGGGAGCASALH
                     AHAQGANVLLATKLRLGDSNSVMAQGGMQIAVGSDDSPVQHFLDTLKGGHMKNDHQLL
                     KVMVEEGPSIAKWLLELGVLFDRDADGNLHVKKGGGSSKARLLTCSDYTGLEIMRVLK
                     DEVLNQKVPLLEFVAAVELLSDANGACTGAVLKDLDNNRFIVVAAKTVILATGGIGRL
                     HIQGFPTSNHFGATGDALVLAYRLGVPLVQIDTFQYHPSGGVYPEQLVGALVTEGIRS
                     EGGHLVNGKGERFVNELDTRDVVSSSIIRECEEGRGVRTPSGRVGVWLDTPLLDVEHG
                     PGTLDKHFPAMVRQYERYQVDIRKDPVLIYPTLHYQNGGVKIDVNGETPVANLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRTGILAAERSKSLPKSLLTLDHLKRFRAEAKRHH
                     VSAGIISPMVLPAYTNK"
     gene            5154..6329
                     /gene="sucC"
                     /locus_tag="VLA60_02970"
     CDS             5154..6329
                     /gene="sucC"
                     /locus_tag="VLA60_02970"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HSF08346.1"
                     /translation="MNIHEFQAKQLFAQFGIPVPKGKEIKNVKAAEKWASVLDTPVFV
                     VKAQIHAGGRGKAGGVKLTKNKTEVPELAKEILGKTLVTHQTGPKGRKVRRLLIEEGA
                     GIAKELYLSLLVDRESGFPTFIASTEGGMDIEEVAEHTPDKLLKESIDPAVGFQGYNG
                     RNLAFGLGLPELEPAVVKPFFQMLENLYRLFMEKNASLVEINPLVITTDKRLVALDGK
                     VSIDDSALFKHPDIQKFRDLHEEEPLEIEAGSNNLNYVKLDGDIGCMVNGAGLAMATM
                     DVIKLAGSEPANFLDVGGGATKETVAAGFRILLKDKKVKGIFINIFGGIVRCERIAHG
                     VIDAAKEVGIKLPVVVRLQGTNAEEGRKLLAESGLKVEGVADLWEAAQRIVALRKKK"
     gene            6380..7249
                     /gene="sucD"
                     /locus_tag="VLA60_02975"
     CDS             6380..7249
                     /gene="sucD"
                     /locus_tag="VLA60_02975"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HSF08347.1"
                     /translation="MSILVNKNTRVVVQGITGKEGSFHATQCKAYGTQVVAGVTPGKA
                     GQEVEDIPVFNTVREAVKKTEATTSLIFVPPPFAADAILEAADAGIWLIICITEGIPV
                     NDMVRVQRALYGKTTRLIGPNCPGIITAEECKIGIMPGFIHKKGRVGVISRSGTLTYE
                     AVNQLTNLGLGETTCVGIGGDPLIGTGYIDLLHMFQEDDETEAVVMIGEIGGDAEERA
                     AAYIKKEMTKPVVSFIAGITAPPGRRMGHAGAIITGGKGTAADKMAALESAGVRVVKN
                     PAEIGETVKSVLG"
BASE COUNT         1905 a         1630 c         2117 g         1824 t
ORIGIN      
        1 caccgagtca aaagtttaaa aaatccggat aagcgggtga aggagttggt tgggtacgta
       61 aaaagtttga acatggccac tccgcacttg gattttgcat tggaagtcga aaaaattacc
      121 gctgtcaaaa aagataatct cattctcaac gttgatggaa ccatggcggc ggtgttagtc
      181 gatatcggtt ttccggttga tagcctcaat ggctttttca ttttatcgcg aaccatcggg
      241 atgattggac attggacgga tcaaaagcgg caaggcagtc ggctcatccg attgtttgac
      301 tatctggtaa attatgcctc cccgaagcgt cgcgaggtgc caccgttaaa gtaatgaggg
      361 tgtgagcgga cttttttggt tgaatgttat caatcttatc catgttgaac tgaacccagg
      421 agatagtcct atgtccctag aaatggtcca aaaactgtat gcgtcaatgc cgggaattgt
      481 ggacaaagct cgcaaaaaat ttgggcgacc tctcacgctg acagaaaaaa tcctggtcag
      541 ccatgccgat gatttcgata cacaagtctg ggaacgtgga aaggccatgt tggcgcttcg
      601 tcccgataga gtcgcgatgc aggatgcgac ggcccaaatg gccatgttgc agtttatgca
      661 agcggggaaa gataaagtcg ctgttccgag tactatccat tgcgatcact tgatcagggc
      721 tgaagtcgga tcggaaaagg atcttctgcg cgcatgtgat gaaaacaggg aagtctataa
      781 ttttctcgcc tcggccgcga agaaatatgg gatcggattc tggaagcctg gggccgggat
      841 catccatcag gtggtgttgg aaaattatgc atttcctggc ggattgatca ttggaaccga
      901 ttcacacacg ccaaacggtg gcgggttggg aatgttggcc attggcgtcg gtggagcgga
      961 tgcgggggaa gtgatggcgg gtctcccatg ggaggtcctc catcctaaac ttatcggcgt
     1021 gaagttaacc ggaaagctga atggctgggc ttccccaaaa gatgtcattt tgtacatttg
     1081 tggccttctg acggtgaagg gtggaaccaa taagatcgtg gagtattttg gccctggcgc
     1141 ggaaaccatc agtgcgacag gcaaaggtac gattaccaat atgggagctg agcttggcgc
     1201 cacgacttct attttcccgt tcgacgaaaa aatggtggca tacttgaata tcacggagcg
     1261 ggctgacatt gccaaattgg cagctgccaa tcggagcatg ttggttgcag accctgaggt
     1321 ctatcagtct ccggaaaaat actttgatga aatcgtcgaa attgatctgt ccactcttga
     1381 gccgcatgtt gtgggaccgc atacccctga tttggcgcgg ccaatttcaa agatggcagc
     1441 ggaagccaaa gaaaaaggtt atcccgttca attaaaagcg acattagtcg gaagctgtac
     1501 caattcttca tatgaagata tcggtcgcgc ggcgcatatt gccaagcaag ggttacaagc
     1561 tgggcttaaa gccaaaacgg cctttcttgt tagcccggga tcagaacgga tttaccacac
     1621 catgaagcgc gaaggatttt tggagacctt tgaaagtatg ggggccaccg tcctcgcaaa
     1681 tgcctgtgga ccctgcattg gtcaatggaa acgagcggat gccgtgaaag gcaaagccga
     1741 ctctattgtc agtacgttta accggaactt tcctgggcgg aatgatgggg tgagtgagac
     1801 gttatccttc ctggccagtc ctgaagtggt cacggcttat gcgttttcag gtgatctggg
     1861 ctttgatccg attaagggga ccttgaaaac cgcagatgga aaagacttca agtttgaggc
     1921 gcctcaggga gatgaattgc cggccaaggg ttttgccaaa ggggaagaag gtttggttca
     1981 acctgctgag gatggttcga agcttcaagt ggacattcct cctaccagcg agcggttgca
     2041 attattaaag cctttcccaa aatgggatgg gaaagatttt gaaaagttgc cgctattaat
     2101 taaaaccaaa ggcaagacca caacggatca tatttcccca gccggtccgt ggctcaaatt
     2161 caggggacat ttggacaaga ttagcgacaa catgtttctt ggagccaaca atgcttttgc
     2221 agacgaggcg ggcaaggcca atgacgtctt aaccggagag tccggtttga ctcctgcaca
     2281 agtcgccaga cggtataaag aaaaaggttt ggggtcgttt gtggtgggtg atgaaaacta
     2341 tggggaaggg agcagccgtg agcatgcggc gatgtctcca cgatttttgg gtgtcctagc
     2401 tgtcctgaca aaaagttttg ctcgcattca tgagaccaat ctcaagaagc aaggcatcct
     2461 tgcactgaca tttgcggatc cgaaggatta tgaccaaatt gaacaagaag accggatcag
     2521 tgtgaagggg ttaagtggac ttgcgccagg caaaccggtt caggtcgtcc ttcacaagaa
     2581 aaatggtcag gaagtggtca ttcaagccaa tcatagtatg accgaacaac agatcacgtg
     2641 gtttaaggct ggttccgcat tgaatgcgtt aaattaatac ccaattaagt gggccgggtg
     2701 gttgaaacgc acgacgagaa atttgtcaat cgtataaggt actatctttg agctatgcct
     2761 gacacattgg ataaaaaaaa tactctagat caagaagaga gtctccaacc taagatggtt
     2821 accgtggaga ttgaagggaa accgttccag gttcccgccg gtatcaccct gatcaaagcc
     2881 ttgtggtatg cagggcagga tgtgattcgt ggagttggat gcctaggcgg attttgtgga
     2941 gcctgtgcga cttattaccg tacaaaagac gatcctaagg tcaaaacctg tttggcgtgt
     3001 tcgatggcag tcgaggatgg catgtccttt tcgtttatgc cggcttttcc ggcgagaaaa
     3061 gcgacctaca accttcttga gctcaaagac cctaagcagg atttgtttac gctctatcct
     3121 gaagccccct tatgccggaa ctgtaatgcc tgcacagaag cctgccctca acaaattgat
     3181 gtgcgggaag gggtgtggaa agcggtgttt ggggatttta aagcggtctc tgaaatgttc
     3241 atggattgtg tcatgtgtgg aatgtgcgcg cctgtctgta ttgctgatat tgctcccaat
     3301 ctggtcgcgc tctatgccag tcgggctcaa ggcgtgcatt tcaccgaaaa gcctgaaggc
     3361 ctatcgaaac gtattcaaga gattaccgat ggacattttc agcaggaatg ggatcgtatc
     3421 ctcaagttgt ccgatgagga attacaaaat acctccgcgg ccacaaccta accagttttt
     3481 tttgcttttc tcatggaact taccggtcag agaaatttag ttcatcaaac ccgagacgcc
     3541 cgtcggtcta aaagttttcc caagctttct ccagccgaac gggatagcct tatcaagaaa
     3601 taccatcctg atcatcgggc tcatgcctac cggcccatta tttttggccc gaatgccggt
     3661 gaatcaaccg tgaccgaagt ggcgactctg ttggaaggag agagcccggt tccggctgat
     3721 cttaatctta ctcctgatta ctccgtggat gtcctggtcg ttggtggagg aggggcgggg
     3781 tgtgcgtcgg cgctccatgc ccatgcccag ggtgccaatg tgttgttggc cacgaagttg
     3841 cggctcgggg attccaactc ggtcatggcc caaggcggga tgcaaattgc cgttggctcg
     3901 gacgattcgc ctgtccagca tttcttggac accttaaagg gcggtcacat gaaaaatgac
     3961 caccagttgt taaaggtcat ggtggaagag ggaccgtcta tagcgaaatg gctgttggaa
     4021 ttgggtgtct tattcgatcg ggatgccgat ggaaatctgc atgtgaaaaa agggggagga
     4081 agctcaaagg ccaggctgtt aacctgctcc gattacaccg gtttggaaat catgcgagtg
     4141 ttgaaagacg aagtgctgaa ccagaaggtt ccgttgttgg aatttgtcgc tgcggtggaa
     4201 ttgttaagtg atgccaatgg ggcctgcacg ggagcggtgc tgaaagactt agacaacaat
     4261 cggtttatcg tcgtcgccgc taaaacggtg atcctggcaa caggcggaat cggccggttg
     4321 cacattcagg gatttccaac cagcaatcat ttcggggcga caggggatgc cctggtcctg
     4381 gcctatcgcc tcggcgtgcc tctcgttcaa atcgatacct ttcaatatca tccttcaggt
     4441 ggggtgtatc ctgagcaatt agtcggtgcg ctggtgacag aaggcattcg ctctgaaggg
     4501 ggccatttgg tgaatgggaa gggcgagcgc tttgtgaatg aattggatac ccgtgatgtt
     4561 gtgtcctcgt cgatcattcg ggaatgtgaa gaggggcggg gcgttcgaac accatctgga
     4621 agggtgggcg tctggctgga tacgccgttg ttagatgtgg aacatggacc gggaacgttg
     4681 gacaaacatt ttcccgcgat ggttcggcag tatgagcgct accaggtcga tatccggaaa
     4741 gatccggtac tgatctatcc cacgctgcat taccaaaacg ggggcgtcaa gattgatgta
     4801 aatggagaaa cgcctgtggc caatctgttt gtggcgggag aggcctccgg cggattacat
     4861 gggcgaaacc ggctcatggg gaattctttg ttggacttaa tggtgtttgg caagcgaaca
     4921 ggtattcttg ctgcagagcg gtccaagtct ctgccgaaaa gcttgttgac cttagaccat
     4981 ctgaagcgat ttcgagccga agccaaacgg catcatgtgt ccgctggcat catttcacct
     5041 atggtcttgc cggcttacac caacaagtaa ttttcaagtc gattgatcac caaacgggat
     5101 attgtcatct ccgaatgttc cgatttgagc tgtagaacct accaagagga tttatgaata
     5161 ttcacgagtt tcaagccaaa cagctctttg cacagtttgg gattcctgta cccaaaggca
     5221 aggaaatcaa aaacgtcaaa gcggctgaaa agtgggcctc agttctggat acccccgtat
     5281 ttgtggtgaa agcgcagatt catgcgggtg gacgtggtaa agccggcggg gtcaaattaa
     5341 ccaaaaataa aactgaagtc ccggagctgg ctaaggaaat tctcggaaaa acactggtga
     5401 cccatcagac aggtcccaag ggcagaaaag ttcggcgcct attgattgaa gaaggtgcag
     5461 ggatcgctaa ggaattgtat ttgagtttac tggtagaccg tgagtcaggc tttccgacgt
     5521 ttattgccag tacggaaggc ggcatggaca ttgaggaagt ggccgaacat acaccggaca
     5581 aattgctgaa agagtccatt gatccggcgg tcggattcca agggtacaat ggaagaaatc
     5641 tggccttcgg cttgggactt ccagagcttg agccggcggt ggtgaagccg ttttttcaaa
     5701 tgttggaaaa cctgtaccgc ctgttcatgg aaaaaaatgc atcgcttgtg gaaattaatc
     5761 cattggtcat caccacggat aagcgtctgg tcgcgttgga tggaaaagtc tcaattgatg
     5821 acagtgcact atttaaacat cctgatattc aaaagtttag agatctgcac gaagaagagc
     5881 ctttagagat tgaagcgggt agcaataatc tgaactatgt caaactggat ggagacatcg
     5941 gttgcatggt caacggggcg ggtttggcga tggcaaccat ggacgtcatt aagttggcgg
     6001 ggtccgaacc ggcgaatttt ttagatgtgg ggggtggggc gacaaaagag acggtcgcag
     6061 ccggttttcg cattctgttg aaagataaaa aggtcaaggg catattcatt aatattttcg
     6121 gtgggattgt gcgttgcgag cgtattgccc atggagtgat tgatgcggcg aaagaagtcg
     6181 gcatcaagct acctgttgtg gttcgacttc aaggaaccaa tgcggaagaa ggccgaaagc
     6241 ttctggctga atcaggcttg aaagtggaag gggtcgctga cctctgggaa gccgctcaac
     6301 gtattgtcgc tctcagaaaa aagaaatagt taacctggtc gtggaaatat tctccttgat
     6361 tggtgaaagg tgatgtggca tgagcatttt ggtcaataag aatacccggg ttgtcgttca
     6421 agggatcacc ggcaaagaag ggtcatttca cgcgacccag tgcaaggcgt acggaacgca
     6481 agtcgttgcc ggcgtgacgc ctggcaaagc cggtcaggaa gtagaagaca tcccggtgtt
     6541 taataccgtt cgtgaagctg tcaagaagac tgaagccacc acctcattga tctttgtgcc
     6601 acctcccttt gccgccgatg ccattttaga agccgccgat gccggcatat ggctgatcat
     6661 ctgcattaca gaggggattc ctgtcaatga tatggttcgg gtccagcggg cgctgtatgg
     6721 gaagaccacg cgtctgattg gcccaaattg tccgggaatt ataacggccg aggagtgcaa
     6781 gatcgggatc atgccaggat tcatccataa aaaagggcgg gtcggggtga tttcaagaag
     6841 tggcacctta acctacgaag ccgtcaatca gctgaccaat ttaggtcttg gcgaaacgac
     6901 ctgcgtcggc atcggtggcg atcccctcat cggaaccggg tatatcgatt tgttgcacat
     6961 gttccaggaa gacgacgaaa cggaagcagt cgtgatgatt ggggaaattg gcggcgatgc
     7021 cgaggaacgg gctgcggcct atattaaaaa ggagatgacc aagccggtcg tgagtttcat
     7081 tgccggaatt acggcacctc ccggaagacg gatgggccat gccggcgcca ttatcaccgg
     7141 aggaaaaggc accgcagccg ataaaatggc cgccctcgaa tcggctggag tgcgagtggt
     7201 aaaaaaccct gctgaaattg gcgagacggt gaagtcagtg ttgggttgat cagtcgatat
     7261 ttctttccgg acactctcat gtgcccattc ctttaaaggt tcggtagttt ttctatccat
     7321 aagtgaccct gaagtctggt tttgagacgt gccacgcgtg tcctccatgg ctgtctgatt
     7381 ctcaaatttg ctcagctcat cgacggcatt gatttcctac atctcattca catcatcaaa
     7441 gtctccttgg tgagtcgtct ccacgcgcag aggcta
//
LOCUS       DATIAL010000090        16298 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_445259, whole genome shotgun sequence.
ACCESSION   DATIAL010000090 DATIAL010000000
VERSION     DATIAL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16298
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445259"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VLA60_02980"
     CDS             complement(<1..309)
                     /locus_tag="VLA60_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSF08348.1"
                     /translation="MSEVHILRDVLIIFSISVLVVFVFQKLRLPAVAGFLVSGTLVGP
                     HGLNLISDLHQVETLAEIGVVLLLFTIGLETSLSRIRASRRLLWVGGHLQILSMLSAV
                     A"
     gene            complement(556..1191)
                     /gene="leuD"
                     /locus_tag="VLA60_02985"
     CDS             complement(556..1191)
                     /gene="leuD"
                     /locus_tag="VLA60_02985"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HSF08349.1"
                     /translation="MQAFTILTGTVAPLNRGNVDTDQIIPKQYLKTIHRTGLKEGLFA
                     DWRRRADGSPDPDFFVNQPRYQKATILLTRENFGCGSSREHAPWALLDYGIRCILAPS
                     FADIFYNNCFQNGILPVELPGEDIQTLFDRVAHTDQYHVTVDLAQQVVTLPEGEKYSF
                     TLDPFRKDCLLKGLDGIGLTLQHESAIATYEKRRAQEAPWLFQDFSPSDHS"
     gene            complement(1261..2667)
                     /gene="leuC"
                     /locus_tag="VLA60_02990"
     CDS             complement(1261..2667)
                     /gene="leuC"
                     /locus_tag="VLA60_02990"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HSF08350.1"
                     /translation="MASKTLFEKIWDQHVVREEPDGTTLLYIDRHLVHEVTSPQAFEG
                     LRLAGRRPRRPTAALAVPDHNVPTTDRSQGIADPMSALQISTLEGNCAEFGITYYGME
                     DIRQGIVHVIGPEQGLTLPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLV
                     QKRPKTMEIRVEGTLPDYCSSKDVILAIIGQIGIGGGNGFVVEYTGSVIRSFSMEGRM
                     TLCNMSIEAGARAGLVSPDESTVAYVKSRPLSPKGALFQQAKSDWLNLKTDPGARFDK
                     VVELRGEDVSPQVTWGTNPGMVTDVNGHVPDPGSIADEKLRNATERALAYMGLPAGIP
                     ICDIRVDRVFIGSCTNSRIEDLRVAARYVKGKRVAQSVKAMVVPGSGLVKHQAEEEGL
                     DRIFREAGFEWREPGCSMCLAMNADVLTPGERCASTSNRNFEGRQGQGGRTHLVSPAM
                     AAAAAVEGHFVDIRDWKM"
     gene            3085..4122
                     /locus_tag="VLA60_02995"
     CDS             3085..4122
                     /locus_tag="VLA60_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSF08351.1"
                     /translation="MFNYFISHFSQDMAIDLGTSSTLIYIKGKGIVLNEPSVVTVETN
                     SKKLLAVGEEAKRMIGRTPGNLTAIRPMREGVIADFDMTEHMLRHFIQKVHRGGTLLR
                     PRIIIGVPSRITQVEQRAVKESAELAGAREVYLIEEPVAAAIGAGLPITEPSGNMVVD
                     IGGGTTDIAVISLGGIVYSESVRIAGDQLDGAITSYLKREYSLLIGEHMAERIKMEIG
                     SAYPLPEKKQMAVKGRDVVSGIPRTILVDDNEIREALQDCLTTIIRAIRLALENTPPE
                     LAGDIIDRGIVLTGGGSMLQGLDNRLREETSLPIVTVDDPLTSVVLGVGKTLEEFSLL
                     RKISSHSRLNP"
     gene            4265..5020
                     /gene="mreC"
                     /locus_tag="VLA60_03000"
     CDS             4265..5020
                     /gene="mreC"
                     /locus_tag="VLA60_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HSF08352.1"
                     /translation="MGGPFARILEVPLHVVASIQDSIRHGWDRYIALQNVWEENQQLK
                     QEVQRLQGEQNFLREHAIIAMQFQQLLAYQETTPMTTLPARIIGRNVSNWYRAIIINK
                     GTQDGIHPEMGVITDAGVVGRVVRVNPTTAVVLLLSDPNVAITGMIQKSRDEGLVQGT
                     PQGTIHMKYLPPLSPVQPGDPVVTSGLTDDFPRGLQIGRIQQVTKADTDLFQLGEIDP
                     IVDFAKLEAVLVITSFQPDPPSSLPKPPVSPSP"
     gene            5030..5539
                     /gene="mreD"
                     /locus_tag="VLA60_03005"
     CDS             5030..5539
                     /gene="mreD"
                     /locus_tag="VLA60_03005"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HSF08353.1"
                     /translation="MTIFLDILLCFGVVLAQATFSTILSAKGIYPDLCFILACVMGFH
                     SGEYKGLRIGLTVGLFQDLFSPGGIGLNMILKGLAGALAGVTTHTFSTVTSPAILLVS
                     FALSLGCGLASLVVAYPVVDAPILFHALSSGLLPQGLYNSLLTLGMFWVIKRIGPSLS
                     VVHLGRGPR"
     gene            5550..7382
                     /gene="mrdA"
                     /locus_tag="VLA60_03010"
     CDS             5550..7382
                     /gene="mrdA"
                     /locus_tag="VLA60_03010"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSF08354.1"
                     /translation="MLDTSRQANELADIQNRLIFVKIGFLMLLVLLGLRLWQLQVRDG
                     LHYQELARDNRTRSIVLEPARGLLYDRNGELLANNIPSFQLYVSLEDIQDREALLAQL
                     PHYVDIDPQEVSNKLSIKSRRGRVKIKADITLKEAALIESHRLELPGVVIQPEYQRHY
                     PLNTYASHVLGYVGEISESQLKDPEFSHLQGGRIIGQNGVERTFDEYLLGETGEEVIE
                     VDALGYPKRSLSVRRPLAGDDLYLTLDIRLQRLAEDLLGQEAGAIVALDPWNGDVLAL
                     ASKPGFNPNDVSGGISAKDWQRFLKDTRHPLTNRAIQGQYPPGSVFKIIMAAALLETQ
                     TLSAQEMIPCHGTFPFGRRVFRDWKAGGHGQVDLTKAIAESCDVYFYKAGNQLGIDPI
                     ALYARQFGLGEKTGIALPSERSGLVPSSEWKKRVKREPWYPGETISISIGQGFLTVTP
                     IQMAKVASIVATNGRVVQPRLLKATRLRRTGTLKEKPEPPIQQLAIYPQTFAHIKEGL
                     AAVVTKGTAKLAQSEFVSIAGKTGTAQVVALKPDGDKDKNKEPQKEFRDHAWFVAFAP
                     VDHPKIAVAVLVEHMGHGGSASAPLAKTLIEAYMGFETTQEPSL"
     gene            7424..8548
                     /gene="rodA"
                     /locus_tag="VLA60_03015"
     CDS             7424..8548
                     /gene="rodA"
                     /locus_tag="VLA60_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HSF08355.1"
                     /translation="MSLMNDNVNRRGLDTFDWCFLGVIMAIISVGILSIYSVTNPQST
                     TGFPIYLKQATWMVVGSLAFLVAVGIDYHKLARLSYLLYGIGLILLIIVLIAGKTSRG
                     SQRWIPLGPFAVQPSEFIKIPLLLMLAAYYGATTREGWVRRLIIPGLIVLPGFLLILK
                     QPDLGSSLGFLAIFLVVILTVGMKSKAFGFVILAALMLFPFAWGGVWGSLHEYQQDRI
                     LSFVDPNSDPGGKGYHGLQSRIAIGSGQLLGKGLYGGTQTQFKFLPEGHTDFVFAVFA
                     EEWGFIGSIVLLSLYILLFLMGLEIALKAKDIVGTLLAVGVVTMIAFGVVVNIAMTSG
                     LAPVVGIPLPLMSYGGSAMVVNLTALGLLLNVKRRRLSLL"
     gene            8877..10430
                     /locus_tag="VLA60_03020"
     CDS             8877..10430
                     /locus_tag="VLA60_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HSF08356.1"
                     /translation="MGVEIAISVTREETRVAVLDNRVVTELYVDRPRKKDFVGDIYKG
                     KVIKVLPGMQAAFVDIGLDRAAFIHVSDLSVGTEPGDTLVDSEEDERSQEVPRPRRQT
                     ARPIENLLAEGQELLVQISKGPIGTKGPRITTYVSLPGRFLVLMPNVDHIGVSRRITN
                     DEERHRLKDLMRRIRKPEYGYIVRTVCEDVPEEDLQTDVRFLSALWEDTLKAANSQPA
                     PSLLHTDLSLPLRVVRDLFTKKVDRLLIDAKADFEEVKDFVRRYLPEQAARVHFYDKK
                     QEGLFDHLGIELEITRALSRKVWLKSGGHIVIDHTEAMTVVDVNTGRFVGKRDQEETI
                     LKNNLEAAKEIGYQIKLRGIGGIIIIDFIDMERERNREKVYQATLDALAGDKAQTRIS
                     RISDLGLIEISRERVREDLLRTLSEVCSDCEGRGYTKSTMTVAYEIFRDIRRIGMTRG
                     QPQQIVVGANPKVIELIFETEHSSIEQLEQEFQQPILFEADPLLHLEQYDIVVVGKLT
                     SRAESRTAS"
     gene            10673..11809
                     /gene="dprA"
                     /locus_tag="VLA60_03025"
     CDS             10673..11809
                     /gene="dprA"
                     /locus_tag="VLA60_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HSF08357.1"
                     /translation="MTSTLQGWLELLAVKGLGPVTYTRLINRFGSPEAIRSLNTHTLV
                     SMGEISPSLAMAIHQPLSAEAQDHIAKELQAVQDGRYAILTLADAHYPPRLKTITDPP
                     PVLWYTGQLQERDQQALAVVGSRKGSHIGRTFTRQLSGDLAALGFTIVSGLARGIDAA
                     AHEGTLATSGRTLAVLGCGIDRTYPPEHGPLRQRIEQQGAVLSEFPMGTPPHSYHFPQ
                     RNRIISGLSLGVIVAEATSRSGSLITARMALEQNREVFAVPGNVTNTLTRGPHRLIKE
                     GAKLVENSLDVVEEILPMLEPSFREQLEKQQGAFQPQPSAPALGTEEQHLLDCISLEP
                     VSLDDLINQCAYAPSEVMSILLSLEIKGLIKQIPGLQYLRMCIG"
     gene            12433..15051
                     /locus_tag="VLA60_03030"
     CDS             12433..15051
                     /locus_tag="VLA60_03030"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF011313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HSF08358.1"
                     /translation="MSKSLIIAEKPSVAADIAHSLGGLKREKDYFEGDDYVVTSAVGH
                     LLEIKAPDDVEVKRGKWSFANLPVIPDHFDLNPIARTEDRLKLLAKLISRKDIDRIIN
                     ACDAGREGELIFRLIVQHTKAKQPISRLWLQSMTPKAIREAFEELREDEQMKPLADAA
                     RCRAEADWLVGINGTRAMTAFNSQDGGFYLTTVGRVQTPTLAIVVEREEQIKQFVSRP
                     YFEVHAQFDVEAGQYSGRWFDTNFKKNDEDAERRAERLWERNQAEAIRQACQGQQAVV
                     TEETKPTTQSAPMLFDLTSLQREGNARFGFSAKTTLSLAQALYERHKVLTYPRTDSRA
                     LPEDYLSTVQDTLGILRDEASERLSAMTGGSGGNPIGPHAAKILSNDWIQPNKRIFND
                     AKISDHFAIIPTLQAPKNLSEAEAKIYDLVVRRFMAVFFPPAEFRNTTRISQIDRHHF
                     KTEGKVMVKPGWLAIYGREAIAGEGTLVAVGENEKATVSDIEIRDMATKPPARYTEAT
                     LLSAMEGAGKLIDDDDLREAMSGKGLGTPATRAAIIEGLISENYLLRKDKSLMPTTKA
                     FQLLTLLRGLGVTELTMPELTGEWESKLSQIERGQLDRKAFMEQISKIVEQIVARAKN
                     YGATTVPGDYSTLQSPCPKCGGLVKENYKRFSCTQCDFSITKIPGSRQLEVPEAESLI
                     AERTIGPLQGFRSRLGRPFAAILKLNDEHKLEFDFGQNDDDENCEPVDFHGQESLGSC
                     PKCQSSVYEHGMNYTCEKAVGPNKTCDFRTGKVILQQEIESAQIRKLLSEGRTELLTG
                     FVSSRTRRKFKAYLKCGDDGKVGFEFEARKPKTGDDQAASGKKLPAKKAETKKPSAKK
                     SATKKPARTAKKVKPR"
     gene            15171..15359
                     /locus_tag="VLA60_03035"
     CDS             15171..15359
                     /locus_tag="VLA60_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08359.1"
                     /translation="MVQKGTKKGKTFYSWSNFPTCTVAIWDRPINRPCPQPGSIFDRK
                     NQETKRKQNIPSQCGMRV"
     gene            complement(15399..>16298)
                     /gene="clpX"
                     /locus_tag="VLA60_03040"
     CDS             complement(15399..>16298)
                     /gene="clpX"
                     /locus_tag="VLA60_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSF08360.1"
                     /translation="KTLFAQTLAHYLDVPFTIADATTLTEAGYVGEDVENIILKLLQA
                     ADYEVERAERGIVYIDEIDKITRKADSPSITRDVSGEGVQQALLKLIEGTVANVPPQG
                     GRKHPHQEFIQVNTTNILFICGGAFVGLDSIIERRLNQKRMGFGADVQIRGQHQIGDL
                     FSKVEPEDLLQYGLIPEIIGRLPIVAALDQLDEPALVRILTEPKNALIKQYQKLLSLD
                     KVKLKFADGALLAIAKKAHLQKTGARGLRAVLESVMLDLMYELPSQAEVKEVLVTEEF
                     IHGKGEPIKVFEHQKGIKPAVGS"
BASE COUNT         4235 a         4285 c         3933 g         3845 t
ORIGIN      
        1 cgccacggca ctcagcatgc ttaaaatttg cagatgacca ccaacccata agagccttct
       61 ggaagcccgt atgcgagaaa gagaagtttc caatccaatg gtaaacaaca acaacacgac
      121 accgatttcg gcgagagttt cgacttggtg cagatcagaa atgaggttca ggccgtgagg
      181 cccaactaat gtgccagaaa ctaaaaatcc cgcaaccgct ggtagccgga gtttttgaaa
      241 aacaaaaaca accagaacgg aaattgaaaa aataatgagg acatcccgga ggatgtggac
      301 ttcactcatc tttcagagct gcggctctgg gcaatgacaa tcattcagga tacttccagc
      361 tatatgcgag aacaggaaaa gggagacggg gatggtctcg agaacccatc atccctcgaa
      421 ttgaggataa tgtctaccat ccgctgcgtc ccattcgacg ggcaggggcc aaagctagtc
      481 cggctaggag cggcaggaga atcgaaatgg atccttccag gcccatcccg attagtcgag
      541 aaggtcaaga gactttcatg agtggtctga aggcgaaaaa tcctgaaata gccagggagc
      601 ctcctgagcc ctgcgttttt cataggtggc aatggcagat tcgtgttgaa gcgtgagccc
      661 tattccgtct aatcccttta acaagcaatc cttgcgaaat ggatccaagg tgaacgaata
      721 cttctcccct tcaggaagag tgaccacctg ctgagccaag tcgactgtga cgtgatattg
      781 gtcggtatgg gctacccggt caaataatgt ttggatgtct tctcctggaa gctcgacggg
      841 gagaatccca ttctgaaaac aattattgta gaaaatatcg gcaaaactcg gagccagaat
      901 acaacgaatg ccataatcca acaaggccca tggagcatgc tcccttgagg atccacaccc
      961 gaaattttca cgggtcaaca gaatcgtggc cttttgatag cgtggttggt tgacaaaaaa
     1021 atcaggatcc ggagagccat ctgcccgtcg ccgccaatcg gcaaacaatc cctctttgag
     1081 gccggtgcga tggatcgttt tcaaatattg cttcgggatg atttgatccg tatcgacatt
     1141 gccccgattt aacggagcga ccgttccggt caatatagta aacgcctgca tatctatctc
     1201 ccgtgttatg ctagaggaag taatggccat cctgaagaca aaagaattgc cttagtaacc
     1261 tcacatcttc caatcacgaa tatccacaaa atgtccttca accgccgccg cagcagccat
     1321 ggcaggggag actaagtgcg tccgtccgcc ctgaccctga cgcccctcga agtttcgatt
     1381 actggttgag gcacatcgtt ctcccggtgt aagcacgtcc gcattcatgg cgaggcacat
     1441 actgcacccc ggttcgcgcc attcaaaacc cgcctcgcga aaaatccgat ccaacccttc
     1501 ctcttccgct tggtgtttga ctaaccctga acctgggacc accatagcct tgacgctctg
     1561 agcgacgcgt tttcctttga cataccgtgc cgccacccgc aaatcctcaa ttcgcgagtt
     1621 tgtgcaggac ccgatgaaaa cccggtcgac ccggatatca catataggga tgccagctgg
     1681 caaccccata tatgctaagg cccgttctgt ggcgttccgc aatttttcat ccgcaatgga
     1741 tccaggatcg ggcacatggc cattcacatc agtcaccatt ccaggattgg tcccccaagt
     1801 cacttgagga gaaacatctt cccctcgcag ctcaacaacc ttatcaaacc tggctccagg
     1861 gtcggttttc aaattcagcc aatccgactt ggcctgttga aacagcgctc ctttcggact
     1921 gaggggtcgg ctcttcacgt atgcgaccgt cgattcgtct ggactgacaa gaccagcgcg
     1981 cgctcccgct tcaatagaca tattacaaag ggtcatccgg ccttccattg aaaacgatcg
     2041 gatcaccgaa cccgtatatt ccacgacaaa accgtttcca ccaccaatac caatttgccc
     2101 gataatcgcc agaatcacat ctttggaaga acaatagtca ggaagcgtgc cctccacacg
     2161 aatttccata gtttttggtc gtttctgaac caagcattgc gtcgccagca catgctcgac
     2221 ctctgaagta ccaataccaa acgccaaggc gccaaacgct ccatgagtag aggtatgaga
     2281 atctccacac acaattgtca tccccggtag cgtcaaccct tgttcaggtc cgatcacatg
     2341 aacaatgcct tgccgaatat cctccatccc ataataggtt attccaaatt ctgcgcagtt
     2401 gccttccaag gttgaaattt gcaaggcact catgggatcg gcaatgccct gcgaccggtc
     2461 ggtggttgga acattgtgat caggaacggc aagcgccgca gttggacgtc gtggccttct
     2521 tccggctaac cgaagtcctt caaaggcttg aggagaggtc acttcatgca ccaaatgtcg
     2581 gtcaatatat aataaggtag ttccatccgg ttcttcacga accacatgtt gatcccaaat
     2641 cttttcaaac aatgttttgc ttgccatgga atgtatcctt ctttttgaaa attttcggac
     2701 tctaattatc tacattcatc cccttctggc aagagccagg aaaagttttt tccagagttg
     2761 ttttcatatc cgccttttct ttgtggtctt ttaacacttc tgatatactt tcgcctgcga
     2821 caggggacaa tccaacctta tggctccacc ttctaactac ttaggtggaa ataagtgagt
     2881 gccgacaacg attcatccag cccagaacca aaattaagtg gtttccggcc aatcgcaaac
     2941 aacgaccgaa cgtcgataca tccataaaat ggcaaaggtt atctttttcc cctggagcgc
     3001 agaaagactc taacaatcct aattccgatt ccgccattgt cgcccaccct ctgaaattaa
     3061 taggtctcaa tacttcccat gggtatattc aattatttca ttagccattt ttcccaagac
     3121 atggccatcg atctgggaac ctcttctacc ttaatttata tcaaggggaa agggattgtc
     3181 ctgaacgaac cttccgtcgt gacagttgag acaaactcca aaaaacttct cgcggttggg
     3241 gaagaagcca agcgaatgat cggacgaacc ccgggtaatc tcaccgccat tcgtcccatg
     3301 cgagagggtg ttattgctga ttttgacatg acggaacaca tgttgcgcca ctttatccaa
     3361 aaagttcatc gtgggggaac tctcctcagg ccacgcatta ttattggtgt cccgtccagg
     3421 atcacgcaag tcgaacaacg tgccgtcaag gaatcagctg agctcgctgg tgcccgtgaa
     3481 gtttatctca ttgaggaacc ggtggccgct gccattggag caggattgcc gattaccgag
     3541 ccttccggca acatggtcgt cgatatcgga ggaggcacta ccgacatcgc cgtgatctcc
     3601 ctaggaggca tcgtttacag tgaatcggtc cgtatcgcgg gcgatcaact agatggagcc
     3661 atcacaagct atctcaagcg ggaatacagc ttgttaatcg gcgaacatat ggcagaacgc
     3721 atcaagatgg aaatcggctc agcctaccct ctgcctgaaa agaagcaaat ggccgtcaaa
     3781 gggcgagatg tggtgtccgg catccccaga acgatccttg ttgacgacaa tgagatacga
     3841 gaggcgttac aagattgcct caccaccatc attcgagcca tccgtctggc cttggaaaac
     3901 actccgcctg aattggcagg ggatattatt gaccggggga tcgtgctgac cggaggcgga
     3961 tcgatgcttc aagggctgga taatcgccta cgtgaagaaa cgtccttacc cattgttacg
     4021 gtcgatgacc ccttaacctc ggttgtcttg ggagtaggga aaactctgga ggaattcagc
     4081 ctccttcgga agatttcttc gcattccagg ctgaatccct gagcttccct caatttgcat
     4141 gcctcgatca gagacaaatt ttcccatatc cacaagtatc cggcgagttc taggaattat
     4201 cttcattcta ttcctcgccc tcctgctgtt tctgccgcga caatctcagg aatggttgag
     4261 tcatgtggga gggcctttcg cacgaattct ggaagtcccc cttcatgttg tcgcctcgat
     4321 acaggattct atccgtcacg gttgggaccg ctacatcgcc ctacagaatg tctgggaaga
     4381 aaaccaacag ctaaagcagg aagtacagcg cctacaaggg gagcagaatt tccttcgaga
     4441 acatgccatc attgccatgc aatttcaaca attgctggcg tatcaggaaa ccacacccat
     4501 gacgaccctt ccggctcgaa tcatcggccg gaatgtgtct aattggtatc gggcgataat
     4561 catcaataaa ggcacccaag acggcattca tccggaaatg ggagtcatca ccgatgccgg
     4621 agtcgtaggt cgtgtcgtac gagtaaaccc cacgacggcg gtcgtgcttc tgctttccga
     4681 tcccaacgtc gcgattaccg gaatgattca aaaaagccgg gatgagggtc tggtacaggg
     4741 cacccctcaa ggaaccattc atatgaaata tctgcctcct ctatcgcctg tccagccggg
     4801 agatccagtg gtcacatctg gattaacgga tgattttccc cgtggactcc aaattggccg
     4861 gattcaacag gttaccaaag ccgataccga cttatttcaa ttgggtgaaa ttgatccaat
     4921 tgtcgatttt gccaaactgg aggcggttct ggtcattacc tcgtttcagc ctgatccccc
     4981 ttcatcttta ccaaaaccgc cggtctctcc ttctccctaa tataatctcg tgactatttt
     5041 tctcgacatc cttctgtgct ttggagtggt tctggctcaa gccaccttta gcacaattct
     5101 ttccgccaaa gggatttacc ctgacctatg tttcattcta gcctgtgtaa tggggtttca
     5161 ctcaggagaa tacaaaggac tcaggatcgg actgacggtg ggacttttcc aagatttatt
     5221 cagtccaggc ggcattgggc tcaatatgat cctaaaagga ctcgcgggag ccttggccgg
     5281 cgtcacaacc cacacgtttt ccaccgtcac cagtcccgca attttgcttg tcagcttcgc
     5341 cctttcgtta ggatgcggat tggcctctct cgttgtagca tatccggttg tggatgcccc
     5401 gatccttttt cacgccctct cctccggact tctcccgcaa ggactctata atagcctcct
     5461 gaccttggga atgttctggg ttattaagcg cattggccca tcgctgagtg tggtacactt
     5521 aggacgggga ccacgataag acccgcacaa tgctggatac ttcccgccag gctaatgaac
     5581 ttgccgatat tcaaaatcgg ttaatattcg tcaagatcgg ctttctgatg ctattggtgt
     5641 tgctggggtt acgcctttgg caattacagg ttcgagacgg attgcactat caagaacttg
     5701 cccgcgacaa tcgcactcga tccattgtct tagaaccggc tcgaggactt ctgtatgatc
     5761 ggaacggcga actcctcgcc aataatatcc ccagcttcca attgtatgtt tccttggaag
     5821 acatacagga ccgtgaggcc cttttagctc agttacctca ttacgtagat attgacccac
     5881 aagaggtttc caataaactt tcaataaaaa gccgaagagg ccgagtcaag atcaaagccg
     5941 acattacgct gaaagaggcg gcactcatag aatcgcaccg acttgaactc cccggtgtgg
     6001 tgattcagcc ggaatatcag cgtcactatc cacttaacac ttacgcctcg cacgtcctgg
     6061 gttacgtggg tgaaatctcc gaatcacagc ttaaagatcc ggaattttcc catcttcagg
     6121 gaggacgcat catcgggcaa aatggagtgg agcggacatt tgatgaatat cttcttggag
     6181 aaaccggaga ggaagtgatt gaagtcgatg cgctggggta tccaaaacgg tctctttcag
     6241 ttcgacgtcc cctggccggg gatgacctgt acctgacgct cgacattcga cttcagcgcc
     6301 tcgccgagga cctacttggg caggaagcgg gggccattgt tgccctggat ccctggaatg
     6361 gggacgttct ggctctggcg agtaaacccg ggtttaatcc caatgatgtt tcgggaggga
     6421 ttagtgccaa ggactggcaa cgatttctca aagatacacg ccaccctctc accaatcgcg
     6481 ccattcaagg ccaatatcct ccagggtcgg tatttaaaat cattatggcg gcggccctct
     6541 tggagactca aaccctcagc gcacaggaaa tgataccctg ccatggaacc tttccctttg
     6601 gaaggcgggt ctttcgtgat tggaaggcgg gaggccacgg gcaggtcgac ttaaccaaag
     6661 ctatcgcgga atcgtgcgat gtctactttt acaaagccgg caatcaatta ggcatagatc
     6721 ccatcgccct ttatgctcgt caattcggat taggtgaaaa aaccggcatt gcgctccctt
     6781 ccgaacggtc cggccttgtc ccttcctcgg aatggaaaaa aagggtcaaa cgggaaccat
     6841 ggtatcccgg cgaaaccatt tccatttcta ttggacaagg atttttaacc gtgactccca
     6901 ttcagatggc gaaagtggct tccatcgtag caaccaatgg acgagtggtc caaccccgct
     6961 tgctcaaagc cactcgactt cggcgaaccg gcacactcaa agaaaagcct gagccaccta
     7021 ttcagcaatt ggccatttat ccccaaacct ttgcccatat caaggaagga ttggcggcag
     7081 tcgtcacgaa gggaacagcc aaactggcac aatcagaatt cgtgagcatt gcaggcaaga
     7141 ctggaacggc acaagttgtt gccctgaaac cggatgggga caaggataaa aacaaggagc
     7201 ctcaaaaaga atttcgagat cacgcgtggt ttgtggcctt tgctcccgta gaccacccga
     7261 agattgccgt ggcggtatta gttgaacaca tggggcatgg aggctcagcg tccgccccgt
     7321 tggccaagac actcatcgaa gcctatatgg gttttgagac cacccaagag ccttctcttt
     7381 aatgaggaat ccgctctctt aaggatggag aacaacgcat ctcatgtcat taatgaacga
     7441 caatgtgaat cgccgtggac tggatacctt cgattggtgt ttcctgggtg tcattatggc
     7501 aattatctct gtgggcatcc tatcaattta tagcgtgacc aaccctcagt cgacgaccgg
     7561 attccctatt tatcttaagc aggccacatg gatggtcgtc ggctccttag cctttctggt
     7621 cgcggtcggt attgattatc acaagctcgc ccgtctttcc tatctgctct atgggatcgg
     7681 actgatctta cttatcatcg tgttaatcgc aggaaaaacc agtcggggct ctcaacgatg
     7741 gattcctctc ggcccctttg cggtccagcc ctcagaattc attaagattc ctttgctact
     7801 catgctggct gcctactatg gggcaaccac ccgtgaaggc tgggttcgtc gattgatcat
     7861 acccggactg atcgtacttc caggattttt actcatcctc aagcaacctg acctgggaag
     7921 tagcttaggc tttctggcca tttttcttgt ggtgattctc acggtgggca tgaaatccaa
     7981 ggcctttgga tttgtgattt tggcggcact gatgttgttc ccctttgcct ggggcggggt
     8041 ctggggctcc cttcacgagt accaacagga tcggattctc agctttgtcg atcccaattc
     8101 tgatccaggc ggcaaaggtt atcacgggct tcaatcgcgc atcgccatcg gttcaggcca
     8161 attactggga aagggattat atggaggaac gcaaacccag tttaaatttc ttcctgaggg
     8221 ccacaccgat tttgtgtttg cggttttcgc agaggaatgg ggctttattg ggtcaatcgt
     8281 cttactttcc ttatatatcc tcttattttt gatgggttta gaaatcgcgt tgaaagccaa
     8341 agacatcgtc ggcactctct tggctgtcgg agtagtaacc atgatagcct ttggagttgt
     8401 cgtcaatatt gccatgacat ccgggctggc ccccgttgta ggcattcctc ttcctctcat
     8461 gagctatgga ggcagcgcta tggttgtcaa tctcacagct ttggggctct tgctcaacgt
     8521 gaaacggaga cgtctgtcac tcctttaggt atcatgtacc ctgtggctcg atttcgatag
     8581 cgtgggccag gttatcgcac aaagagggaa acaacggaga attcgattca gaaaagtccc
     8641 agagccgtgg attttttcac atacttgcgg gaacaaggaa gtgaacacac agaaccatcc
     8701 tcaaatggtt ccataacagt attcatttac aattattgac agaatgggca caaagctatt
     8761 tcagattttg acaggcagat ccaacaaatt ggctcagcca catcagcgac atctcttcac
     8821 caagaatgtt gacaaagaat agcttctggt cccttctgag aatgtaggag atcactatgg
     8881 gagtagaaat tgcgattagt gtcactcgtg aagaaacacg ggttgccgtg ctcgacaatc
     8941 gtgtcgtgac agaattgtat gtcgaccgtc caaggaaaaa agattttgtg ggggacattt
     9001 acaaaggcaa agtcattaaa gttctgccag gtatgcaagc ggcctttgtc gatatcggac
     9061 tggatcgggc cgcattcatc cacgtatctg atctgtcggt cggaacggaa cccggggata
     9121 ctcttgtgga cagcgaagag gatgaacgca gtcaggaggt gccgcgccct cgacgccaaa
     9181 cagcacgtcc catcgaaaat ctgttagcag aagggcaaga actcctggtc caaatttcca
     9241 aaggccccat cggcaccaag ggacctcgaa ttaccacgta cgtctctctc cccggacgat
     9301 tcttagtgct catgcccaat gtcgaccata ttggtgtgtc gcgaagaatt acgaacgacg
     9361 aagagcgtca tcggctgaag gacctcatgc ggcgcatccg gaaacccgaa tatggatata
     9421 ttgtcaggac cgtgtgcgaa gatgtacccg aagaggatct gcaaacagac gtcagattcc
     9481 tgagcgcgtt atgggaagac acgctcaaag cggccaacag ccaaccggct ccgtcccttt
     9541 tgcatacgga tctgtctctc ccgctgcggg tggtacgaga cttattcacc aaaaaagtcg
     9601 atcgactatt gattgacgcc aaagcggatt ttgaggaagt gaaggatttt gtccgtcgct
     9661 atcttccgga acaagcggcg cgcgttcatt tctatgacaa gaagcaggaa gggttattcg
     9721 atcacctggg aatcgaatta gaaattacca gagccctgag tcggaaagtc tggcttaagt
     9781 ccggaggaca cattgtcatt gatcacacag aggccatgac cgtggtggac gtcaatacgg
     9841 ggcggtttgt gggaaaacgc gaccaggaag aaacgatttt aaaaaacaat ctggaagccg
     9901 caaaagaaat tggctatcag ataaaattac gaggcattgg cggcattatc atcattgatt
     9961 tcattgacat ggaacgggaa cggaaccgtg aaaaagtgta tcaggctacc ctggacgccc
    10021 tagcgggtga taaggctcaa acacgtattt cccgcatatc cgatcttggg ctcatcgaga
    10081 tttcccgtga acgtgtccgg gaagatctat tacggacatt atcggaagtt tgcagtgact
    10141 gtgaggggag ggggtacacc aaatccacca tgacggtggc gtatgaaatc tttcgggata
    10201 tccggcgcat cggaatgact cgtggacaac cacaacaaat tgtggtcggg gccaacccca
    10261 aagttattga gctcatcttc gaaaccgaac acagcagcat tgagcaactc gaacaggaat
    10321 ttcaacaacc aatattattt gaggcggatc cattacttca ccttgagcaa tatgacatag
    10381 tcgtggtggg aaaattgact tcacgcgccg aatctcggac cgcatcctaa ccacgagaga
    10441 acgattgaat catgaacgat gccctccacg attctttctc attatccgta acacttgtct
    10501 ccaggaacag aaaggaatgg ggacccagat cccccctcca acgctggaaa aatcaggagg
    10561 tcttcattct tcatagtcca acctggaaca gtttctgggt ggagagccat gcaggcagat
    10621 catttcacga tgatctccgg actcgtattc gccatcatcg caaaggatcc ggatgacctc
    10681 aaccctgcaa ggctggttgg aacttctggc agtcaaagga cttggccctg ttacttatac
    10741 cagactcatc aaccggttcg gctcaccaga agccattcgc tccttgaaca cccatacact
    10801 cgtatcaatg ggtgaaattt ctccatcatt ggccatggcc attcatcaac ccctatctgc
    10861 tgaggcacaa gatcacattg ccaaagaatt gcaggccgtg caggatgggc gatacgctat
    10921 cctgacactc gcagacgctc actatccgcc ccgattaaaa accattacgg atcctcctcc
    10981 ggtactctgg tataccggcc agctccaaga gcgtgatcaa caggcccttg ccgtcgtcgg
    11041 ttcaagaaaa ggctcgcaca tcgggcggac attcacacga caactgagcg gcgatttagc
    11101 tgcactgggc tttacgattg tgagtggcct ggccagaggc attgatgccg cggcccacga
    11161 aggcacactg gctacatctg ggagaaccct ggcggttctt ggatgcggca ttgaccgcac
    11221 ctatccgcct gagcacggtc cgctacgaca acgcattgaa cagcagggag ccgttctctc
    11281 tgaatttccc atgggaaccc ctccacattc ttaccatttt cctcaacgta atcggatcat
    11341 tagtggactc tcacttggtg tgatcgtcgc ggaggccacc tcgcgcagtg ggtccctcat
    11401 taccgctcgg atggccttag agcaaaaccg tgaagtcttt gccgtaccag gaaatgtgac
    11461 caatacgttg actcgtggcc ctcaccgcct aattaaagag ggagcgaaat tagtcgaaaa
    11521 ttctttagat gttgtggaag aaattcttcc tatgttagaa ccctcattcc gggagcaact
    11581 cgagaaacaa caaggcgcgt ttcagcctca accatccgcg cctgcactag gaacagagga
    11641 acaacatcta ttggattgca tttcactgga acctgtttcc ctggatgatt taatcaatca
    11701 atgcgcctat gccccatctg aagtcatgag cattctcttg tctttggaga tcaaagggct
    11761 tataaagcag ataccgggcc tacagtatct ccggatgtgc atcggatagt ataccagcat
    11821 gtcttttccc tctggaaaat tttactcgta aacaattcat gattttcatc atggtctatg
    11881 cctaaatcac tcgtcatcgt cgaatcgcct accaaagccg aaaccctgtc caaatatttt
    11941 ggatacaatg accaagcctc ggcatctgtg ggtcatctta aaggcgtccc gaagagcaac
    12001 ctgggcattg acctcgaaca caactttggg gattctgtcg caaaaaatag atttgtaaga
    12061 tacgccaggg gttttcaggt tggcccgagt tgaaaaaacc agtaaagata gccattttca
    12121 tctgtcagac acggagcaaa aaccccttat tgccttcaaa aaaaatttgc gacagaaccg
    12181 tcggtaggct ggccttggcg gctggccgga acggctgcgg ccgctggaaa aaatgagttg
    12241 aatacccagc ctaacttgcc tctaccctac gccatcatta tgatgagcac gcgaaaaaac
    12301 cagtcacatc gcagcaggtg gattgtgacg acaccgtttt caccctgagt ttgtagtgga
    12361 tcttacacgc gtgagtatcg gtttcccgta catcaaagat accaaaggag ccttccctgg
    12421 tagtggttgc taatgagtaa atcgctgata attgccgaga agccctctgt tgcagccgac
    12481 atcgcgcatt cgcttggcgg actgaaacgc gagaaggatt attttgaagg tgacgactat
    12541 gttgtaactt cagccgttgg gcacctactt gagatcaagg cgcctgacga cgtagaggtc
    12601 aaacggggca agtggtcttt tgccaacctg ccggtcattc ccgatcactt tgacctgaac
    12661 ccaatcgccc gcaccgagga ccggctcaag ttgctcgcca agctgatcag ccgcaaagat
    12721 atcgaccgaa ttatcaacgc ctgtgacgcc gggcgcgaag gcgagctgat cttcagactg
    12781 attgtccaac acactaaggc taagcaaccg ataagccggc tttggctgca gtccatgacg
    12841 cccaaggcca ttcgcgaagc atttgaggaa ctgcgcgagg atgaacaaat gaagccgctc
    12901 gctgatgccg cccgctgccg ggcagaagca gactggctgg ttggcattaa cggcacccga
    12961 gctatgacgg cgttcaactc ccaagacggg ggtttttacc tcaccaccgt tggccgggtt
    13021 caaacaccca ccctggccat cgtcgtggag cgcgaggaac agatcaagca attcgtctcg
    13081 cggccctact tcgaagtgca tgcccagttt gatgtcgagg ccggacagta ttcaggtcgc
    13141 tggtttgata cgaacttcaa aaagaatgac gaagacgcgg aacggcgcgc agagagactt
    13201 tgggaacgca atcaagctga agcaatcagg caggcctgcc agggtcagca ggcggtggtc
    13261 actgaagaaa ccaagccgac cacgcaaagc gccccgatgc tgtttgacct gactagtctg
    13321 caacgtgaag gcaacgcgcg gtttggcttt tcggccaaga caacattatc gctggcgcaa
    13381 gcgctctatg aacgccacaa ggtgttgacg tatccaagaa ccgattcgcg tgctctgccg
    13441 gaagactatc tctctaccgt gcaagatacc ctgggcattc tgcgtgatga ggcaagtgag
    13501 cgtttgtcag ccatgaccgg cggtagcggc ggcaatccca tagggccgca tgcggcgaaa
    13561 attctgagca atgactggat ccaaccgaac aaacgtattt tcaatgacgc gaaaatttcc
    13621 gatcacttcg ccattatccc cacattgcag gcacccaaga acctcagcga agccgaagcc
    13681 aagatatacg acctggtcgt acgtcgattc atggcggtct ttttcccacc tgcggagttt
    13741 cgaaacacca cccgtatctc acagattgac aggcaccact tcaagaccga aggcaaggta
    13801 atggtcaaac cgggttggtt ggccatttac ggccgtgaag ccattgccgg tgaaggcacg
    13861 ctcgtcgcgg ttggggagaa tgaaaaagct acggttagcg atattgaaat tcgcgacatg
    13921 gcgaccaaac caccagccag atataccgaa gccacactgc tttctgcgat ggaaggcgcc
    13981 ggcaagctga ttgatgatga tgacttgcgc gaagccatgt ctggaaaagg tctgggcaca
    14041 ccggcaaccc gcgcagcgat catcgaaggc ttaattagcg agaactatct attgcgcaaa
    14101 gacaagtcac tgatgcctac caccaaggcc tttcaactgt tgaccttatt gcgcggcctg
    14161 ggggtgaccg aactcacgat gcctgaactg accggcgaat gggaaagcaa gctttcgcag
    14221 atagaacgag gtcagttgga ccgcaaagcg ttcatggaac aaatcagcaa gatagttgaa
    14281 caaattgttg cgagggccaa aaactacggc gcaacgaccg ttccaggcga ttactcaaca
    14341 ttgcagtcac cctgccccaa atgcggcggc ctggtcaaag aaaactacaa acggttctcc
    14401 tgcacacaat gcgatttttc aattacgaaa attccgggca gccgtcaatt ggaagtaccc
    14461 gaagcagaat ctctgattgc cgaacgcacg attggtccgt tgcagggatt tcgcagtcgg
    14521 cttggccggc cgtttgcggc aatcctcaaa ctgaacgatg aacacaagct tgaatttgac
    14581 ttcggtcaaa atgatgacga tgaaaactgt gaaccggttg attttcacgg ccaagaatca
    14641 ctggggagtt gcccgaagtg tcagtcatcg gtttatgaac atggaatgaa ttatacctgt
    14701 gaaaaggcgg tcggcccgaa caagacttgc gattttcgaa cgggcaaagt catactgcaa
    14761 caggaaatcg aatccgctca gatcagaaaa ttgctgagcg aaggcagaac cgaattgctg
    14821 accggctttg tttcatcacg aacccgtcgc aagttcaagg cttacctgaa atgcggagat
    14881 gacggaaaag tcggcttcga atttgaggcc cggaaaccca agaccggcga cgaccaggct
    14941 gcgagcggca aaaagttgcc cgcgaagaag gccgagacca agaaaccgtc ggcaaaaaaa
    15001 tcggctacga agaaaccagc tcgtacagcc aaaaaagtca aaccccgcta atgatcgggg
    15061 gaacgcggcc gattttgaaa tttcctcacc tgctctcaat accccgaatg taaaaccaca
    15121 aaacccttaa gctaagcgtc aaatgcgctg tcaaggattg cgggggagac ctggttcaaa
    15181 agggcacaaa aaaaggcaag acattttatt cctggagcaa ttttcctacc tgcaccgttg
    15241 cgatctggga tcgacccatc aatcgtccct gcccacaacc aggctccatt tttgatcgaa
    15301 aaaatcagga aacaaagagg aagcaaaata ttccgtcaca atgcggaatg cgggtatgag
    15361 aatatagcga caccaacgga gaagagcccg gaaccagctt aagatccgac ggcgggcttg
    15421 atccctttct gatgctcaaa gactttgata ggctcacctt tgccgtgaat aaactcttcg
    15481 gtcaccaaca cctctttcac ctccgcctgt gacggcagct catacatcaa gtccaacatc
    15541 accgactcta agaccgcccg aagtccacgc gctcctgttt tttgcagatg ggcctttttg
    15601 gcaatcgcca ataacgcccc atcggcaaac tttaatttca ctttatccaa ggacaataat
    15661 ttctgatatt gcttgattaa ggcgtttttg ggttccgtta aaatgcggac caatgccggt
    15721 tcatccaatt gatcaagagc cgcgacaatc ggcaggcgac cgataatttc aggaattaac
    15781 ccatattgga gtaaatcttc cggttccact ttggagaata agtcaccaat ttgatgttgc
    15841 ccacgtatct gtacatcggc gccaaagccc atccgctttt ggttcaacct ccgctcaatg
    15901 atggaatcca accccacgaa cgccccacca caaatgaata atatattggt cgtattgact
    15961 tgaataaact cctgatgagg atgctttctt cccccttgag ggggcacatt ggccacggtc
    16021 ccttcaataa gtttcaacaa tgcctgctgg acaccctcac cggagacatc acgggtaatc
    16081 gaaggactat cggccttgcg ggtaattttg tcaatttcat caatatacac aattccccgt
    16141 tctgcacgct caacttcata gtccgcagcc tgcagaagtt ttaagataat attttcaaca
    16201 tcttcaccga catacccggc ttccgtcagc gtcgtcgcat ctgcaatcgt aaatggtaca
    16261 tccaggtaat gggctagtgt ctgggcaaat agagtttt
//
LOCUS       DATIAL010000091         5511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_982900, whole genome shotgun sequence.
ACCESSION   DATIAL010000091 DATIAL010000000
VERSION     DATIAL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982900"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VLA60_03045"
     CDS             complement(<1..1238)
                     /locus_tag="VLA60_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08361.1"
                     /translation="MSSLTPKDELHELLLDRLDATTAHGLDEQLADPRLRVPVLELLI
                     ELKEISSKIQGEAVWALGEVYRKRCLDSAIPWLDLGITFAQASGALGLRYFKESPMIL
                     GFLEKESNRDELLAHVLELADGSNEAAPQCAYEWFKVLPQLCGEIALPEIREWARLGM
                     ELAEWNYVLGNEFFRECPSIAKAIPLGSAKSWIGFGMKLMVQNSLGKPDYIGTLEFFR
                     ASPSLFLEIHDENVKQGVIDLGTNLADHSPEQAVVLLAKAPEVLARVSMVEWKIRILK
                     FGLLVADRDPEATLAYFTQVPEVVVLTGKEDDSGVFDAWFGQGMEALEYSVEAGRAFF
                     GLETRQACSAVEQAMRGVSLRQVARSLKMFARALCGEDVAIEGLPEGGGSIGASHMST
                     SPVSGKAQVSADGKTVYLPLV"
     gene            complement(1444..1770)
                     /locus_tag="VLA60_03050"
     CDS             complement(1444..1770)
                     /locus_tag="VLA60_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HSF08362.1"
                     /translation="MEWARCLIEKSEGIEIRIYIQPRASKTEIVGLYGEALKIRVASP
                     PINGQANAELCRFLARHVGIPRQYVQLKSGVSSRQKRVLIEGKTLSDVAAALTESLSS
                     KNSEHP"
     gene            complement(2443..3195)
                     /locus_tag="VLA60_03055"
     CDS             complement(2443..3195)
                     /locus_tag="VLA60_03055"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HSF08363.1"
                     /translation="MHRLDGRTKVLTVLGVFGLALCFSDPFYLLVLLGFVMSGLALSG
                     AWANVRKMWMLTVLLFVYSVALWPFFVEGVTPVPLLHGLGVTWEGVMVGLGMGLRLLI
                     MLWAGIWLLSTMSVEELAQASQQLGLPSRAGFVFSLAFRWVGMLLGAGAGVVQAQRSR
                     GLDVSTGGILQRIRKYVPLVIPLIGHALRQTNLLAMSLESKGFHPLATRHFYSTGKLD
                     TQDYAVMSVMLILVVVGIWLRWQGIGTLDVRF"
     gene            complement(3309..>5511)
                     /locus_tag="VLA60_03060"
     CDS             complement(3309..>5511)
                     /locus_tag="VLA60_03060"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HSF08364.1"
                     /translation="GPAAAWGAGIGNVIGDCFGTLGPASFFGLLGNFIFGYLPYVLWG
                     HLGWFSSGQPPLGKSWRQMMEYGVVCVIASAACAGMIAWGVEWLGLLPFVILAPAIFF
                     NNVVMGLFLGPPLLGFLYPRVQRWRLRYEDIRESDSSHIHLSKTLRSHESMGSEKAND
                     HLDDAIVECRGLSFQYASGSAPVLRNVSFSLASGELVVLLGRSGSGKSTICYACNGLI
                     PHMIPGIFSGTLRVKGRETVDDPVWKQAGRVGLVFQDFDTQLVATTVEGELLHPLEYR
                     DPLLSSDEVRRRVVHALSQIGLGNCAHRDPMRMSGGQRQRLVMASVLVQEPALLVLDE
                     PGSDYDPAGRAELREVLRNLRQEGITVLMTEHDDGYLLQADRVLVLDQEQIVWEGKPE
                     ALLRQPRLMRDYGLRPFALTECFEEWGVEHLPISVEEAWTQAEVLNLRLSPPAAVLDD
                     SIRLGHLPERKSTMALPLIQVDNVSFRYEEQTVLNEVSFTICPGEFLALLGQNGSGKS
                     TLARLLNGLLLPTHGTIIVEGLDTRTTSMNELARRVGLVFQNPDHQIFADTVWEEVAF
                     SAKNMGCSSDEIVDRVQESLAAVGLPFEGSRDLDPFSLRKGERQRIAVASVLATRPAV
                     LIVDEPTTGLDPEETDRMMAMIRRLNQQGHTIVMITHSMGLVAAYARRCLLIHSGRIL
                     ADGTPREIFADPGLIQSASLEIPAISRFSQRWGQTLLTVEEVKASCRPGFP"
BASE COUNT         1557 a         1589 c         1144 g         1221 t
ORIGIN      
        1 accagaggaa gatataccgt tttcccgtcc gcgctcacct gagcttttcc ggagacagga
       61 ctcgtggaca tgtgactggc accgatcgaa cctccgccct ccgggagtcc ctctatggcc
      121 acatcctctc cacacaacgc cctggcaaac attttcaacg atctggcgac ctgacgaagc
      181 gacactcccc tcattgcctg ctctacggct gaacaggcct gccgcgtttc caaaccaaaa
      241 aaggctcgtc cagcctcaac actatactca agggcctcca tcccctgacc aaaccacgca
      301 tcaaacacac cggaatcgtc ttccttgccg gtcaagacga ccacctcggg aacctgagta
      361 aaatacgcga gagtagcctc gggatcccga tccgccacaa gaaggccgaa tttcaagatt
      421 cgaatcttcc actcgaccat cgagacccta gccaaaactt cgggagcctt cgccaataaa
      481 actaccgcct gttcgggaga atgatctgcg aggtttgtac ctaggtcaat gaccccctgc
      541 ttgacattct catcatggat ctcaagaaac aaacttgggc tggcacgaaa aaactccaag
      601 gttccaatgt aatcaggctt tccaaggctg ttttgaacca tgagtttcat gccaaaccca
      661 atccaggact ttgccgatcc caacggaata gctttagcaa tggagggaca ctcccggaaa
      721 aattcatttc ctaatacata attccattcg gccagctcca tccctagtcg ggcccattcc
      781 cgaatctcag gaagggcaat ctccccgcac aattggggaa gtactttgaa ccattcatac
      841 gcacattgtg gagcggcctc atttgaccca tcggccaact ccaagacatg agccaacaat
      901 tcgtcccgat tcgactcttt ttccagaaat ccgagaatca tagggctttc tttgaaatat
      961 cgaagaccga gtgcgccaga ggcctgagcg aatgttatcc ctaaatccaa ccacggtatg
     1021 gcactgtcta aacatcgttt tcgatacacc tcacccaggg cccatacggc ttcaccctgt
     1081 atttttgacg aaatctcttt taattcaatg agtaactcta gaacaggcac ccggagacga
     1141 gggtccgcga gttgttcatc gagaccatgc gccgtggtcg catcaaggcg gtcgagtaat
     1201 aactcatgaa gttcatcttt tggggtaagg gaagacatgt aattgattaa taaccgaggg
     1261 agttaaatgg atacaagaag gattcttacc cagcacattc ccataccgca ttgtaaccac
     1321 aactacggct gggcattata aaagtggctt caagggttgt aaactcaccc aactatcttg
     1381 cagattccct ctttatactc ccaacaccca cttctatcta cctgcgttta ggtgtcccgt
     1441 acctcatgga tgttcagaat ttttcgagga gaggctttcc gtcagggctg ctgctacgtc
     1501 ggataaggtt ttcccttcaa ttaagactcg cttctgtctt gaactaactc cggatttcag
     1561 ctgcacatat tggcggggaa taccaacatg tcgtgccaaa aatcgacaca attccgcatt
     1621 ggcctgacca tttatcggcg gagacgctac gcgaatcttc aaggcttccc catagagccc
     1681 aacaatctcc gtttttgacg ctcgtggctg aatataaata cgaatttcta taccttctga
     1741 tttttctatc aggcacctgg cccattccat gtaaaatttc ctataaccga taaaaataat
     1801 tgagcttttt ttgtgggata aaaccactga tccgataaag ataaacaaaa aaacaatatc
     1861 acataaatag caataacata ttaactttta ataccttatg tcaggcacca agaccttgac
     1921 atgaacccag ttggtgagta gaatattttt agattgataa cggttatcat tttcgaaagt
     1981 aaaatcattc agtaacggat tttttatgtt catctgtatc tgcaaaggta tcaaagaatc
     2041 tgatgtccgt gaacttggac aagcaggtgt cacttgtccc ataaagcttg cggacgccct
     2101 cgaactcaat gacaaacggc agtgctgtgg ccgttgcatc aaaaagatat caaaatttgt
     2161 cgcactcgct gaagaagaaa cgtcctgccg acacaccccc atggtaagtt aggggttccg
     2221 ttcctaccac cgcccacccg attcccagca aattcttctc aggttcctca cctgacctct
     2281 ccacgagaaa agcattgtgc ctaaagcttt ttggggaacc aatcttgcca gggtcaacct
     2341 catccagcca gcaattgccg catctcaaaa tggatggagg actcgttttg ttatccttgc
     2401 ctataggaag acgcccggcg aaatgatgca gacctcgaat tcttaaaatc tcacatcaag
     2461 cgttccaatg ccctgccatc gcaaccaaat gcctaccaca accaaaatca gcattacact
     2521 cataaccgca tagtcttggg tatccaattt ccccgtagag taaaaatgcc gcgtcgctaa
     2581 gggatggaac cctttggatt ctaaagacat ggccagaaga tttgtttgac gaagggcatg
     2641 gccaatcaat gggatcacca gaggcacata tttgcgaatg cgttgcagaa ttccgcccgt
     2701 cgacacatct aatccgcgtg agcgctgcgc ttgaaccacg cccgcccctg ctcccaacaa
     2761 cattcccacc caccgaaacg caagtgagaa aacaaaccct gcccgtgaag gaagccctaa
     2821 ttgctgagag gcctgcgcga gttcttcaac cgacatcgtc gaaagtagcc aaattccagc
     2881 ccagagcata attaacaatc gcaagcccat gcctaaacca accatgacgc cttcccaggt
     2941 cactccaagc ccatgcaaga ggggcacagg tgtcacacct tcgacaaaga aaggccagag
     3001 cgccacgcta taaacaaata ggagaaccgt cagcatccac attttcctca cattggccca
     3061 cgcgccggac aaggctaacc cgctcattac gaatcccaat agtaccagca agtagaacgg
     3121 atccgaaaaa cagagagcta gcccaaacac cccaagcacc gtcagaactt tggttcgtcc
     3181 gtctaaccgg tgcatccacg aattccggaa aagatacaag gaaacaatca tccgtattta
     3241 ccaggcccta cgaaactcac gcatcccaaa aacgctctcc attttcttaa aagtatcggc
     3301 cacaagtttt atggaaaacc tggtctgcag gaagccttca cttcttccac cgttagcaag
     3361 gtctgtcccc atcgttgact aaagcgagaa atggccggaa tctctaatga tgcggattgg
     3421 atcaagcccg gatcagcaaa aatttcacgg ggggtcccat cagcaagaat tctcccgctg
     3481 tgaattaaca aacaacgccg ggcataggcg gcaacaagcc ccatcgaatg ggtgatcatc
     3541 acaatcgtat gcccttgttg gttcaatcga cgaatcatcg ccatcatccg gtcagtttcc
     3601 tcaggatcaa gtcctgtcgt cggctcatca acaatcaaca cagctggtct ggtagccaac
     3661 acggatgcga ccgcaatccg ctgtcgctct ccttttctta gggaaaacgg atctaagtct
     3721 cggcttccct caaatggcaa tcccacagca gccagcgatt cttgcacccg atcgacaatc
     3781 tcatcgcttg agcaacccat attcttcgcg ctgaaggcca cttcttccca gacggtatcc
     3841 gcgaaaattt gatgatcagg attttgaaag actaatccga cgcgcctcgc caattcattc
     3901 atcgatgtgg tgcgggtatc cagaccttcc acaataatgg tgccgtgtgt cggcaacaag
     3961 aggccattga gcaatcgtgc aagcgtactt tttcctgacc cattctgacc cagcaaagcc
     4021 agaaattctc ctggacagat ggtaaatgac acctcattca ggaccgtctg ttcttcatac
     4081 cggaaagaaa cattgtcaac ctgaatcaag ggcaaagcca tggttgactt tctctccggc
     4141 agatgaccca aacgaatcga gtcatccaag accgccgccg gaggagacag acgaagattc
     4201 aatacctcag cctgggtcca ggcctcctcg accgaaatag gcaaatgttc cacgccccat
     4261 tcttcaaaac attctgtcag ggcgaagggt cgaagaccat aatcccgcat caacctaggc
     4321 tgtcgcaaca aggcttcagg ttttccctcc cagacgattt gctcctgatc taaaaccagt
     4381 acccgatctg cttgcaaaag atagccatca tcatgctccg tcataagcac agtaatcccc
     4441 tcttggcgaa gattacgtaa aacctcccgc aattctgctc gccctgccgg atcataatct
     4501 gaaccgggtt catctaagac caacagcgcc ggttcctgga cgagcacaga agccatcacc
     4561 aaacgttgcc tctggccacc ggacatcctc atgggatcac ggtgggcaca attgcccaac
     4621 ccgatttgac tgagggcatg aaccacccgc cgtcggacct cgtccgaaga aagcaggggg
     4681 tcacgatatt ccaatgggtg aagcagttcc ccctcaacag tcgtcgccac aagttgcgta
     4741 tcaaaatctt gaaacaccag gccgacccgt ccggcctgtt tccacacggg atcatccacg
     4801 gtttcacggc ccttcacccg caatgttccg gagaaaatcc cggggatcat gtgaggaatg
     4861 agaccattac aggcatagca tattgtggat tttccacttc cactcctgcc taacaggaca
     4921 acgagttccc cagaagccag agaaaaggaa acattccgga gaaccggcgc tgaacccgag
     4981 gcatattgga aggaaagtcc acgacattcc acaatagcgt cgtcgagatg gtcattcgcc
     5041 ttctcactcc ccatcgattc atgtgaccgc agggttttgg aaaggtgaat atgggaagaa
     5101 tctgattctc gaatatcttc ataccgtaat cgccaccgtt gcaccctcgg atagagaaaa
     5161 cccaataatg gaggaccgag aaacaatccc attaccacat tattaaaaaa gatcgctggg
     5221 gcgagaataa cgaagggtaa caaccccaac cactccactc cccacgcaat cattccggca
     5281 caagcagctg aagcaatcac acacaccacg ccatattcca tcatctgtcg ccaagatttt
     5341 cccaaaggcg gctgcccgga ggagaaccac cccaaatgcc cccacaatac atacggaaga
     5401 tacccaaaaa taaaattgcc taataatcca aagaaacttg ccggccctaa ggtgccgaaa
     5461 caatctccga tgacattgcc aatacctgca ccccaggccg cggcgggacc a
//
LOCUS       DATIAL010000092         4457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1306180, whole genome shotgun sequence.
ACCESSION   DATIAL010000092 DATIAL010000000
VERSION     DATIAL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306180"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VLA60_03065"
     CDS             <1..667
                     /locus_tag="VLA60_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF08365.1"
                     /translation="IDVERHFMGGYAIVGGHLPLAVGLAFASRYRKEDRVVLCFFGDG
                     AVPSGHTHEAFNLAALWKLPVIFICENNRYGMGTPVEREMLLYKDIAERARAHGIEAE
                     QVDGMDVLAVKELTGRVVKKLRKEPQPYFIEASTYRFMGHSMSDPAHGHYRTKEEVGE
                     ARKQDPLIFLKTRILQEGLATEADFKQMDKDVIKTVTESVKFADESPFPEPGALHEDI
                     LVE"
     gene            765..1733
                     /locus_tag="VLA60_03070"
     CDS             765..1733
                     /locus_tag="VLA60_03070"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HSF08366.1"
                     /translation="MLISYREALNQAMREEMQRDSRVFLIGEEVGYYQGAFKVSKGFV
                     EEFGVERVLDTPITEAGFTGLAIGAAMAGLRPIVELMTFNFGILALDQIVNNAAKIRY
                     MSGGQLSAPMVIRGPGSAAHQLAAQHSQSLEAWFCHVPGLKVIAPATPSDAKGLLKSA
                     IRDENPVIFIEAQLLYGTKGEVAEQDYTLPIGKAAVTRTGSDVTIVAYSKMALLALEA
                     AEALSKEGINAEVIDLRTLKPLDTETLLESVKKTGRVVIVEEGWEFCGLGAQIAETIY
                     SKGFDFLDGPIVRVAGEEVPMPYTRPLEDLAIPDQARVISAVKQIL"
     gene            1830..3134
                     /locus_tag="VLA60_03075"
     CDS             1830..3134
                     /locus_tag="VLA60_03075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HSF08367.1"
                     /translation="MITRVVMPKLTDTMEEGVVVAWKKHEGDAVDSGDILAEIETDKA
                     VLDLESFGSGVLKKVLVPEGETVKAGKLIAVIGEEDDDIEATLAETSEDQAAAREPQK
                     DSAAPSRKEKPSPPSPSPSIPNKVDKSSSAKKEIPETKADTSPEAKFKVSPRAKALAK
                     EQGIDLSTIKGSGPEGRIVEADIQGRVEAPKVLERETKDVPLSQMRKAIARVTTSSKA
                     PVPHFYLTTEVAMDQAERLRKQMEEVNNSPLSLTVLFVRAAALALVRHPEINVSFMGE
                     SLRRHATIDIGIAVALEEGLITPVIRDCAGKNILQVAEEIRELFVRAKGQQLAPEEYA
                     GATFSISNLGMYEVDNFIAVLMPPQAASLAIGAVNIVPVARGRAIKLSRRMKVTLSCD
                     HRALDGAQGARFLQSFKRALEKPLELFLPLKTPSTHHKQAGQ"
     gene            3370..4029
                     /locus_tag="VLA60_03080"
     CDS             3370..4029
                     /locus_tag="VLA60_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSF08368.1"
                     /translation="MMTALLIVVGGQDFGMPEESSRDLDGALLQAAERNDAAMVRQLL
                     QNGASLHAQDEKGQTALLLAVDRNHVESAKVLIEAGADVNTQDRQLDSPLLLAGARGY
                     LEILKLILGAKPDFAIYNRFGGTALIPACERGHVEVVRELLQTDVDIDHVNRLGWTAL
                     LEAIILSDGGPRHQEIVRLLIQAGADINLPDRDGISPLQHARNKGFTPIVHMLESAGA
                     R"
     gene            4122..>4457
                     /locus_tag="VLA60_03085"
     CDS             4122..>4457
                     /locus_tag="VLA60_03085"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HSF08369.1"
                     /translation="MIGKKNLHFTSGKGWYLSGMVTLSLSGLFAAGALGAELPREEVL
                     PLSLATKAASAAVEQCTKDGYQVSAAVVDRAGVVRALLREDGAGPHTVDSSRKKAYTS
                     ASLKRPTAEL"
BASE COUNT         1082 a         1024 c         1304 g         1047 t
ORIGIN      
        1 catcgatgtc gaacgtcatt ttatgggcgg gtatgccatc gtcggtgggc atttaccgct
       61 tgcagtcggg ctggcgtttg cctcacggta tcgcaaggaa gaccgggtgg ttctctgctt
      121 ttttggtgat ggagcggttc ccagcggaca tacgcatgaa gccttcaatc tggctgcgtt
      181 atggaagtta cctgtgatct ttatctgcga aaacaaccgg tacgggatgg ggacgccggt
      241 tgaacgcgag atgcttctgt ataaagacat tgccgaacgg gccagggcgc atggcattga
      301 agcggaacag gtggatggga tggatgtgtt ggcggtgaag gagttgactg ggcgtgtcgt
      361 caagaagttg cgaaaggaac ctcaaccata ctttattgag gcgtcgacct atcgatttat
      421 gggccattcc atgtccgatc ccgcccatgg ccattatcga acgaaggaag aagtgggaga
      481 agcacgcaag caggatccat tgatttttct caagacccgg attcttcagg agggtttggc
      541 tacggaggcc gactttaagc agatggataa agacgtgatt aagacggtga ccgagtctgt
      601 gaagtttgcc gatgaaagcc cgtttcctga gcccggcgcg ctccatgagg atattttagt
      661 cgagtgaaaa ttgtgccctt acaaaggcta agccggaagt attgggctgg gattttcttt
      721 atcatgaaac acgatgatca accattcttg aatgatgacg aaccatgttg atttcctata
      781 gagaagcctt gaatcaggcg atgcgtgagg aaatgcaacg ggattctcgc gtctttttaa
      841 tcggggaaga ggtcgggtac taccaggggg cctttaaagt gagcaaaggc tttgtggagg
      901 aatttggcgt tgagcgggta ttagatactc cgattaccga agccggattc accgggttgg
      961 ccattggcgc ggcgatggcc gggttgcgtc ccattgttga attgatgacc tttaatttcg
     1021 gcattcttgc ccttgaccaa atcgtgaata atgcggcgaa aattcgctat atgtccggag
     1081 gccagttgtc ggctcccatg gtgattcgcg ggccggggag tgcggcccat caattggccg
     1141 cccagcattc gcaaagtctt gaagcctggt tctgccatgt tcccggactg aaagtgattg
     1201 cacccgcaac gccctctgat gcgaaaggat tattgaagag cgccattcgc gatgagaacc
     1261 cggttatttt tatcgaagcg caacttctct atggcacgaa aggcgaggtg gctgagcagg
     1321 attacaccct gccgatcggc aaagccgctg tgacccgaac aggaagcgat gtgaccattg
     1381 tagcctattc aaagatggcc ttactggcgc ttgaggctgc ggaagcgtta tcaaaggaag
     1441 gcatcaatgc cgaagtcatc gacctgcgga cattaaaacc cttggatacc gaaaccctcc
     1501 tggaatctgt caaaaaaaca ggacgtgtag tcattgtgga agaggggtgg gagttttgcg
     1561 ggttgggcgc tcagattgct gagaccatct attcaaaagg atttgatttc ctggatgggc
     1621 ctattgtcag ggtcgctggt gaagaagtcc ccatgccgta tactcggccg cttgaagatc
     1681 tggccattcc agatcaggct cgggttatat cggcggtgaa acagatttta taacacgcaa
     1741 catatttttg aaatggttgt tcctcagaca gtcatcacgg caaattcttt cgaaggattc
     1801 ccctgtcata tcataagtag gagaggccta tgattacgcg cgttgtcatg cccaagctca
     1861 ccgataccat ggaagaagga gtcgtggtgg cctggaaaaa gcatgagggg gatgctgtcg
     1921 actcgggaga cattctggcg gaaattgaaa cggacaaagc ggtccttgat ctggaatctt
     1981 ttgggtccgg tgtgctgaaa aaagtgttag tgccagaggg ggaaaccgtt aaagccggga
     2041 agctcattgc cgtgatcgga gaagaggacg atgacattga ggccacgctg gctgagactt
     2101 ctgaagacca ggctgcagca cgtgaacctc aaaaagattc tgctgctcct tctcgaaagg
     2161 aaaagccttc tcctccttcg ccgtccccct ccatcccgaa caaggtcgat aaatccagca
     2221 gtgctaaaaa ggaaatcccg gaaactaagg ctgatacgag cccggaggca aagttcaaag
     2281 tgtcgccccg ggccaaggcg ttggcgaaag aacaggggat tgatttgtca acaatcaaag
     2341 gcagcggccc tgaaggccgg attgttgaag ccgatattca aggacgggtt gaggcaccca
     2401 aggttcttga gcgagaaacc aaggacgttc ccctgagcca aatgcgcaag gcgatcgctc
     2461 gagtgaccac gtcaagtaaa gcccctgtcc ctcattttta tctcacaacc gaagtggcca
     2521 tggatcaggc cgagcggctt cggaagcaga tggaggaagt gaataactcc cctctgagtt
     2581 taacggtctt gttcgttcgt gcggctgccc tggctttggt ccgtcatcct gaaatcaatg
     2641 tttcattcat gggggaaagc ttacggcggc atgccacgat tgatatcggg atcgcggtgg
     2701 ccctggagga gggactcatt actcctgtga tacgggattg tgccgggaaa aatattctgc
     2761 aagtcgctga agagattcgt gagttgtttg tccgggccaa aggccaacag ctcgctccgg
     2821 aagaatatgc cggggccacg ttctccatat caaatcttgg gatgtatgag gtggacaatt
     2881 tcattgcggt cttgatgccg ccccaagcgg cttctctggc cataggcgcg gtaaatattg
     2941 ttcccgtagc acgaggacgg gccattaaac tgtcccgccg aatgaaagtc acgctctcct
     3001 gcgatcatcg agcgcttgat ggtgcgcaag gggcacggtt tctccagtca tttaaacgtg
     3061 cgctggaaaa acccttggag ctttttcttc cgttaaaaac cccatcaaca catcacaagc
     3121 aggctggcca ataattggtg tgccggtatt ccgaagtttc aaggaagaag tcattggttg
     3181 ccattcgtca gtgagcggag agtgtgccgt gctgaaaatc ctccattctc cttctctttt
     3241 ttttcttgcc cgacccaggc cttgtcgtac ctcgtcccct gtgctgactc gacggtcagt
     3301 gaagcgggac acgccatggg aagtcaggat tgaccgcaga taacatgcat gaaaacaata
     3361 ggacttctca tgatgacagc tcttctgata gtggttggcg gacaggactt tggcatgcct
     3421 gaagagtcaa gccgagatct ggatggagcg ttgcttcaag cagcggaacg aaatgatgca
     3481 gccatggtcc gccaattgtt gcaaaacggg gcaagccttc atgctcaaga tgaaaaaggg
     3541 caaacggcgt tactcctggc cgttgatcgg aatcatgttg aaagcgcaaa ggtgttgatt
     3601 gaggctggag cggatgtgaa tacgcaggat cgtcaactgg acagtccatt gcttttggcc
     3661 ggagcgagag gatacctcga gattttaaag ttgatcttag gggccaaacc cgattttgca
     3721 atttataacc ggttcggggg aacggccttg attccggcat gtgaacgcgg acatgttgag
     3781 gtcgtcaggg aattattgca aaccgatgtg gatatcgatc atgtgaatcg attgggttgg
     3841 accgccttgt tggaggccat tatcctgagt gacgggggac ccaggcatca ggaaatcgta
     3901 cgattgctta ttcaggcggg tgccgatatc aacttgccgg atagggatgg catcagcccg
     3961 cttcagcacg ctcgaaataa ggggttcacg cccatcgttc acatgctgga atccgcaggc
     4021 gcccggtagg gatgtgagat atattgacaa cgttgcagcc tgaacaagac aatcgtcagg
     4081 tcagcacatg aatgatctca tgtttatcaa aggagcgttt catgatcggc aaaaaaaacc
     4141 tgcattttac atcgggaaaa ggttggtatc tatctggaat ggttaccctc agtctaagcg
     4201 gactgttcgc tgcgggcgcg ttgggggctg agctgccgcg tgaggaggtg ctgccactct
     4261 cactggccac gaaggccgca agtgcggcgg tcgaacaatg caccaaagat ggatatcagg
     4321 tgagtgcggc ggttgtggat cgggccgggg tggtgagggc gttattacga gaggatggtg
     4381 ccggtcccca tacggtggat agcagccgca aaaaggccta tacctcggcc agtttgaaaa
     4441 ggcccacggc tgaattg
//
LOCUS       DATIAL010000093        17563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_660862, whole genome shotgun sequence.
ACCESSION   DATIAL010000093 DATIAL010000000
VERSION     DATIAL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 17563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17563
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660862"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VLA60_03090"
     CDS             <1..699
                     /locus_tag="VLA60_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08370.1"
                     /translation="EHPGLPIQVILAPKESPLAQEAPEWMSGYAVSQTSTIVLLTDRS
                     LTYPNDSLNEVFLHEISHILAHRAAGEQPLPRWFDEGLAMMTARRWDLEDRARLVWAM
                     VSGTQVSLNELNTLFSKDETSVRRAYVLAYAFTRDLLEHTGQDTPKRILAKILQGMSF
                     PEAFAQTTFMTLAQAEEQFWSRQTLWNRWIPVATSSGMVWLAITLLAFWAFTRQRKRA
                     AAIKKQWREDDFDL"
     gene            1181..1933
                     /locus_tag="VLA60_03095"
     CDS             1181..1933
                     /locus_tag="VLA60_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF08371.1"
                     /translation="MSQRLKDKVAVITGGNSGIGLATAKLFQAEGARVIITGRRKEAV
                     EAAVKEIGGSSAGFVSDTGNLNDIANLYQQIHKTAGRIDVLFLNAGIAIFGPFDTVDE
                     ATFDRMVNVNFKGLFFNIQKASPLLHEGASIIINSSIADQKGFPNSSVYGATKAAVRN
                     LARTLSTEFLERKIRVNALAPGPIDTPIFEKVGVPAEEVPGMKASFGDQNPMKRMGTS
                     HEVATAALFLASDDSSYITGIDLTVDGGATQL"
     gene            complement(2027..3178)
                     /locus_tag="VLA60_03100"
     CDS             complement(2027..3178)
                     /locus_tag="VLA60_03100"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF08372.1"
                     /translation="MKTNPLGDLSVETFLADYWQKKPLLIRQALPGIKPPIAADELAG
                     LACEEQVESRLIIHDPISDQWELSHGPFTDATFSALPTAHWTLLVQAVDHWVPAAADF
                     LAQFYFIPSWRVDDLMISYSGDKGGVGPHYDNYDVFLVQVSGCRQWEVGGIYDETSPR
                     RSDVPVKILSEWKPEQRWILEPGDLLYVPPRVGHNGIAVGEDCMTCSVGFRAPSHRDI
                     LLDYSESVGEALSEEVRYADPDLVPQANPGQITSEAVRKVQRIFTRYVEDDAKLAQWF
                     GCYMTSPKYQEEETTPEEHRLEEVRTHLTEGGKLVRNEGSRFAFHEHGQNIWLFVDGR
                     QYTCSGLMTNLVKTLCAERQIGSEHYVSSEGHDSLLLDLLSHGSLYLSA"
     gene            complement(3795..4259)
                     /locus_tag="VLA60_03105"
     CDS             complement(3795..4259)
                     /locus_tag="VLA60_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF08373.1"
                     /translation="MANIQWEVKTFQHLTVQQLYDVLQLRVDVFVVEQQCAYRELDEY
                     DRHPEARHISGRNEGGQLIAYARILPPGLRHPEVNLGRFVVKADFRQQGIGHQLLQTA
                     LQNIAGCWPEIPIKMSAQSHLHRFYAQYGFLQVTDGYLEDGIPHVEMVKESC"
     gene            complement(4437..6125)
                     /locus_tag="VLA60_03110"
     CDS             complement(4437..6125)
                     /locus_tag="VLA60_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08374.1"
                     /translation="MMKIGNMNKEGQERYAKVVNWSCLVCLLFFSATGHVLASDINES
                     FDVQVILKQVAGLVSTLKDGPDKYVLSGELGVVQAKVGHIGDASRSLVGIPDGFQNDY
                     QKNRLKKEISLAQARTDKMAEALNVAASISSLEREFTLGGIALEKAKSGDVPGALLIL
                     QRIRMGTNAKADALKAIGEIQAKRGDLRGGIQTALQAADKDPQALWGIAKKQIKLGHL
                     KEMLAEIRRIPPELSRQYALWGVVLAQIDSKDIHGAIEVAKTIPDGHASANAWEDIAF
                     VQIEAGRIQEGLDNLKRALGGASASQNSLAKSDILWRIAVCQAKAGDVASALQTTELV
                     EIVAHKNHALREIAVYQAKAGDVKEALQTASRFNAEISGPPPYVFILQDLARSGKVNQ
                     ALQIADSLDSELEYSPYEAIAVGQAESGDIEGALRTLDAIKWKGEMAPYNRAMAFEKI
                     SYMQVQNGHLEEAVRIGSRIDEPHRARALRDVSLAYVKEGKVDRALEIVSSIKGEQAL
                     ADLSQKIAYAQAFQGDTQGVMKWTQKIASPFVKSTVLLGAVQGILDASNRPILQ"
     gene            complement(6174..7421)
                     /locus_tag="VLA60_03115"
     CDS             complement(6174..7421)
                     /locus_tag="VLA60_03115"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HSF08375.1"
                     /translation="MATIAPFRAVRPKSELASQVAAPPYDVVSLQEARDLADGNPYCF
                     LRIGRAELELGDGVDPYSVEVYQRGADNLRKLIKDGVLVQEPQPLFGVYRQKWGQHEQ
                     TGIVALASVDEYDRGIIKKHEYTRPVKETDRVRIIETHESQSGPVLLFFRQTAKIDQW
                     LKEVTGTKPDVHFVAHDKIEHTVWSVRDPSAIQEVMKNFQDLPALYIADGHHRSAAAS
                     RVRASRGQAGALSAGKHEEGFLAVIFPHDQLQILPYNRVVRDLNGLTPQELLEKLSPH
                     FELQIIARPGDPPAAGFDMYLEGQWHRAKPKQDPSLNSQQDPVRALAVSELTERVLDP
                     LLAIKDQRSDPRIDFVGGIRGTQQLVSLVDSGDWAVAFWLYPTTVEELMAVADADRVM
                     PPKSTWFEPKLRDGLFIHMLRDE"
     gene            complement(7598..8353)
                     /locus_tag="VLA60_03120"
     CDS             complement(7598..8353)
                     /locus_tag="VLA60_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSF08376.1"
                     /translation="MVESGTHISQVTLSAAFGAGLLSVVSPCVLPLVPSHVLYLTGLS
                     IDQLRAPTGRHHVRKTIIVNSLLFIGGFSVVFIAFGLSASLFGQWLINYQDHLRKIGG
                     GLIVLFGLYVLGLFNFGFLSQERRLHFQSHPVGYLGSFLIGVTFALGWTPCVGPILTS
                     ILLYAGTTDTAADGLALLVFYSLGLGLPLLVTAVWLDRFLTHFRQIRNYIRPLSIMSG
                     LLLVAVGGLLYTNSFFLLTSYLERSSIGWYIGQ"
     gene            complement(8397..8990)
                     /locus_tag="VLA60_03125"
     CDS             complement(8397..8990)
                     /locus_tag="VLA60_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSF08377.1"
                     /translation="MRNELYKWTSQKRATFGGSLPGFHRGGRFSAIRSAVAGFMMVGL
                     SLLFGALPVPAASSVPAPEFQLRAFDGHTYSKNSLQGRPTLLVFWAPWCRYCQMELPI
                     LSKFYRGSKPDQLQVLTVAFSDSLAHVEEYVTANPDTFIFPTAYDQDNEVAQAFGVNV
                     TPTFVVMDAQGEMILAHRGAGINQNPQYQKFLKSLKQ"
     gene            complement(9000..9476)
                     /locus_tag="VLA60_03130"
     CDS             complement(9000..9476)
                     /locus_tag="VLA60_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HSF08378.1"
                     /translation="MPNLIPKTFLMLSTLMFACVYGLILSPGLVQADKISGQDNVKVL
                     YHLDSNDPALAKYALALINKHIEAEGGPDKIDLVLVVHGPALKLFESDTVDQELKDKL
                     KTVIDKGIKAEMCQVSMKLYGTPLEKLLAGFTPTEHPVAVKRIADLQRAGYVYIKP"
     gene            complement(9634..9792)
                     /locus_tag="VLA60_03135"
     CDS             complement(9634..9792)
                     /locus_tag="VLA60_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08379.1"
                     /translation="MIETYTGKIGGVFKNVRPARLQPIGRAERTEEYVSTARRRKRRW
                     RQFFNTPH"
     gene            complement(9839..10498)
                     /locus_tag="VLA60_03140"
     CDS             complement(9839..10498)
                     /locus_tag="VLA60_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF08380.1"
                     /translation="MPRRKTNTIRLLLVDDHEVLRLGLKTLFTETESIQVVGEAGTRA
                     AAVSEADRLHPDVVLMDVRLPDGSGIDACREIRNASPDIRVLFLTSFADDEAVMATIM
                     AGAKGFLLKEISGEELVRAVNTVAAGQSILDPAITQRVLTKMQHLSISSADGKKESLA
                     PQELKVLTLVAEGQTNKEIAVALDLSDKTVGHYLENIFQKLQVTRRSQAAAEFVRRFP
                     Q"
     gene            complement(10485..11927)
                     /locus_tag="VLA60_03145"
     CDS             complement(10485..11927)
                     /locus_tag="VLA60_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSF08381.1"
                     /translation="MKPYSIYLVIGGCGMAALIMGVVLPEVLPGWRWAHEPLHSTLET
                     VGGLAAISMGIVLLQRTTDHTRQKFFPLAGGFLGMGILEIFHAISVPGNGFVLLRSLA
                     SLLGGLGFGLMWLSATGKHHTTIKWMPWVMTGGALALGLWTIGFPQYVPVMVREGVFT
                     PTAVAPKSFASMLFFAGSLRFFLDYRTSGRSEDILFASLALMFGFAELMFTYSVIWGS
                     RWWFWHLLRLLVYFFVLGYVGRGYLATVSKLRSSLIQTRRAEQASRQSEQQLRTVLEA
                     RERLAQDLHDGVIQSTFALKLNLERCQRLIPQNPQEALTGISDVLMELKFVIQDLRRH
                     ISGLEPECLNGRQWVEEMRTLVQTVEKSQNLSFHLQIDQEAADLLTPEEATHLLYVVK
                     ESISNSVRHSHATSGTVSLQVREGAVCLEVMDDGLGFDPENSDEGVHGLKNMAARAKR
                     LGAHFQVWSKPHRGTRVIFEIAKKGSHAQA"
     gene            complement(12475..14301)
                     /locus_tag="VLA60_03150"
     CDS             complement(12475..14301)
                     /locus_tag="VLA60_03150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF08382.1"
                     /translation="MSDAKDSYPAQMRFRAVAQSSHDAIIIADQSGTILFWNKGAKDI
                     FGYESEETVGQPLTMLMPDRYRQAHQAGLERYSTRGETRILGETVELSGLRRTGEEFP
                     LELTLSAWKEEERLFFSGIIRDISQRKEAEEALQRSEEKYRAIFNQAVEGIYQATPAG
                     AFLNANAALSHLLGYDSPQALMETVKDIGSQLYVEPTKREEFCRLLEHQDVITDFESQ
                     VYRADGTPIWISENARVIRNAEGAVQWYQGFLVDISGRKQAEALLERQNRLQAENRYL
                     QEEVLEAGAFGDLVGQSPALHNVIRQIDLVAPTEATVLILGESGTGKELVAREIHKRS
                     QRKDRPLIRVNCASIPRDLFESEFFGHVKGAFTGAVKDRAGRFGAADGGTLFLDEVGE
                     IPLDLQSKFLRVLQEQQFERVGEERTRHVDVRVIAATNKDLKQEVENGRFRQDLYYRL
                     NVFPLEIAPLRERKEDIPLLTEYLLGVTSKKLHCAQPKLTKALVGQLQRYNWPGNVRE
                     LQNVIERGLILSERQGLAFDIPQPGGGRASSQPQESHASLMAWSVMTDQEMRLREREN
                     TLAALEQAEWRIYGKGGAAERLGIKPTTLVARIKKMGIVKGG"
     gene            14602..15573
                     /locus_tag="VLA60_03155"
     CDS             14602..15573
                     /locus_tag="VLA60_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSF08383.1"
                     /translation="MKNRHSGRIGLGIALVAMTGSLFTFASQVEAKKVKVELTAVETE
                     VVIDGKGTTYKAWTFNGQVPGPVVRVTEGDQVHFILKNHENNSRPHSMDFHAAETDFL
                     ANYRDVNPGESLEYVWEAKRPGVFAYHCGAFPMIQHIARGMFGAVIVDPKDASAMPKA
                     DREYVLVQSELFTKDPDDVAAMMDAKNDHVVFNGGIFKYDPVHAAKGGEFLTAKPGDR
                     VRFYFVNIGPNNFSSLHPIAEIWDDVWPSGNPANRMQGVQTQVIGAADGAILDVVVEQ
                     KGVYPIVTHSLKDALTGAIALLEVSDEAKDLALMPSVGPKAIDTASK"
     gene            complement(15789..16148)
                     /locus_tag="VLA60_03160"
     CDS             complement(15789..16148)
                     /locus_tag="VLA60_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08384.1"
                     /translation="MGPTKVIPKEGALYEVKTGKLVQDGLPTRTEQEAYVAQHYLVLP
                     VIDKAGKPWALDGQPVFCLHGTQFETVDDQKVHLARCPDCGGMGIRDDEPLVESDCIR
                     CVQCGHEFDTRLEMMEN"
     gene            complement(16744..17400)
                     /gene="elbB"
                     /locus_tag="VLA60_03165"
     CDS             complement(16744..17400)
                     /gene="elbB"
                     /locus_tag="VLA60_03165"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HSF08385.1"
                     /translation="MMKKVAVILSGCGFLDGAEITEAISTLIAIGQNGAAYQVFAPNK
                     DVEETNHLTQKPTGQKRNVLQEAARIARGEIQPLEQLKAKDFDALAFPGGFGAALHLC
                     DFGEKGSGGHIDPQVGRIVKEFNDSHKPIAAICIAPAIMALALGKKGVNVTIGEDAGT
                     ASELEKTGAKHQNCAVEKYVVDHSNKVITTPAYMYGSARPHQIFAGVSGAIAELIKMA
                     "
BASE COUNT         4356 a         4737 c         4168 g         4302 t
ORIGIN      
        1 gaacatcccg gacttcccat tcaagtcatc ctggcgccaa aggaatcgcc ccttgcacag
       61 gaagcgccgg aatggatgtc tggttacgca gtcagtcaga cttccacaat tgtgctcctc
      121 actgaccgga gtttgaccta tccgaatgat tcactcaacg aagtcttcct gcatgaaatc
      181 agccatatcc tggcccatcg ggcggcagga gaacagcctc tgcctcgatg gtttgatgaa
      241 ggattagcga tgatgaccgc ccgaaggtgg gatttggaag accgggcccg attagtctgg
      301 gcaatggtct caggcacgca agtctccctg aatgaactca ataccctctt cagtaaagac
      361 gagacctctg tccgacgggc ctatgtgctg gcatacgcct ttacgcgaga cctcctggaa
      421 cacacaggac aggatacccc caaacgtatt ctggcaaaaa tcctacaagg catgtccttt
      481 cctgaagcct ttgcgcaaac caccttcatg acactcgcgc aagcagaaga gcagttctgg
      541 agtcggcaga cactctggaa tcgctggatc cctgtggcca ccagttccgg aatggtctgg
      601 ctcgcgatca ccctcctggc attttgggcg tttaccagac agcggaagcg agccgcagcc
      661 attaagaaac aatggagaga agatgacttt gatctatgat aggctctaac acgacgatta
      721 cattagacct ttgcaccaat ttagcttcgg ttgagagaag gatgcaattg tataggcatt
      781 tcccttacct tccgactggt gcgaaaaaaa aaggaccata tatctcaagg acatttcatt
      841 aacgggaatt ttttgaattc ctgatagttg ccgggtttcg gcccggcagc cgaggtcctt
      901 ttgtttcggc aaaaggaccc aaaaccagtg acgccccgtc cggtctcatt aagatgggcg
      961 gacgcgagta ttgaggaggg cggcccaact cgctgcgctc aaacaaagtc cgccggttca
     1021 tgagagcgtc cgacccaggg accggacggc agtcgtcagt taaggtcgag agggacacac
     1081 agacaaagaa gccggaaatt aattggaatc actagaaatt atctggcatc aaaaatagcc
     1141 ggtcttgaaa tcatctatac accttgaaag gaaaattcct atgtctcaaa gactcaaaga
     1201 caaagtggcc gtcattacgg gtggcaatag tgggattggt ttggccacag ccaaattatt
     1261 tcaggccgaa ggggccaggg tgatcattac gggaagaagg aaggaggcgg tggaagcggc
     1321 agtgaaagaa atcggaggat cgagtgccgg tttcgtttcg gataccggga atctcaatga
     1381 catcgcgaat ttgtatcaac agatacacaa gacggcggga aggatcgatg ttctcttcct
     1441 caatgccggc attgcgatct ttggcccatt tgacaccgta gacgaagcca cattcgatag
     1501 gatggtcaat gtcaatttca aaggcttgtt cttcaacatt caaaaagcct cgccgcttct
     1561 gcatgaagga gcgtcgatca ttatcaattc gtccatcgcg gaccagaaag gatttcccaa
     1621 ttccagtgtc tatggcgcaa ccaaagcggc cgttcggaat ctggccagaa ccctgtcgac
     1681 tgaatttctt gagagaaaaa tccgggtgaa cgctctggcc cctggaccaa ttgatactcc
     1741 tatttttgag aaagtgggcg ttcctgcaga agaagttcca ggcatgaaag caagctttgg
     1801 tgaccaaaac cccatgaaac gaatgggaac ctcacatgaa gttgccacag ccgcgctctt
     1861 tctcgcatca gatgattcat cttacattac agggattgac ctcaccgtcg atggaggcgc
     1921 cacacaactc taatttttca cagatgatgg cagccaacca gggggcgatc agttgctttg
     1981 gcctgaaagg gagcctctct ttccaggaaa ggcggaaaag tatttttcag gccgaaagat
     2041 agagactgcc atggctcaac aaatcgagga gtaaggaatc atgcccttcc gacgagacgt
     2101 agtgctccga accgatttgg cgttcggcgc acaaggtttt caccagattc gtcatgagcc
     2161 cactgcaggt gtactgacgc ccatcaacaa atagccagat attctgtcca tgttcatgaa
     2221 acgcaaaacg tgagccttca tttctgacaa gttttcctcc ctccgtaaga tgggtacgaa
     2281 cttcttccag acggtgttcc tccggcgtcg tctcctcttc ctggtatttc ggagaggtca
     2341 tataacaacc aaaccattga gccagtttcg catcatcctc gacataccgg gtaaaaatcc
     2401 tctgaacttt tctaaccgcc tcggatgtaa tctggccggg attggcttga ggaaccagat
     2461 ccggatccgc atagcgaacc tcttcgctca acgcttctcc aacagactcg gaatagtcaa
     2521 gcaagatatc ccgatgagac ggcgcacgaa atcccaccga acaggtcatg caatcctcac
     2581 ccacggctat cccgttatgt ccgacacggg ggggtacata aagcagatcg cccggctcca
     2641 atatccaccg ttgctccggt ttccattcgg acaagatttt gaccggaaca tcggagcgac
     2701 gaggagacgt ttcatcatag atgccaccca cttcccattg gcggcagcca ctgacctgca
     2761 ccagaaacac atcatagtta tcataatggg gcccgacacc ccccttgtcg ccggaatagc
     2821 tgatcatcaa atcatcaacc cgccaactgg ggataaaata aaattgcgca agaaagtcag
     2881 cggcggccgg cacccaatga tcgacagcct ggaccaacaa ggtccaatgc gcggttggca
     2941 gtgcggaaaa tgtcgcatca gtaaacggac catgagaaag ctcccattgg tcacttatgg
     3001 gatcatgtat aatcagcctc gattcgactt gttcttcgca agcgagacca gccaattcat
     3061 cagccgcaat cggcggcttg atcccgggca gggcctgccg aatgagcaac ggcttcttct
     3121 gccaataatc tgccaaaaac gtttctaccg acaaatcgcc tagggggttt gttttcatta
     3181 gccaggacaa gccatcagtt taaacagcaa ccacgcaact catctgtggt catcctcaat
     3241 attttcatcc gaaagctatc tgggtcttga gattcttcta acgtttgtat ggtcaccaca
     3301 tttggtagcg agttggacca ctcaaagaaa agcctccgtc tttcagcagc ttcccctggc
     3361 ctggtggtgt agccgagcag tgcagccgac accgtggaaa aggcgcgcat tgtttgagcc
     3421 cttcgacatg actcagggta gactcgcagg tttccgcgac gcctgccgtt cggtcctatg
     3481 ggaggatgct cttattcttg gcgggccttg tctgagcctg gcgagttggc ccgccctctt
     3541 ccctgcatcc gcccaatcca attaggccga acggggcgta aatggttttg ggtccttttg
     3601 ccgaaacaaa aggacctcgt ctgccggggc gaaacccggc aataaacctc atcggttgga
     3661 tatgatcgat agtagtttag ttgaaatgga cgtcctggaa agtgcccaag aactttctgg
     3721 tgccacactg aaatgcctta taaaccataa aaaaacatac aggttcccct agacatgaca
     3781 aacagtcaaa atattcaaca agattctttc accatttcaa catgaggaat gccatcttcg
     3841 aggtagccat ccgtcacttg gagaaatcca tactgcgcat aaaatcggtg gaggtggctt
     3901 tgtgcagaca tttttatagg tatctctggc caacaaccgg ctatgttctg caaagctgtc
     3961 tgtaataatt gatgaccaat cccctgctga cgaaaatcag ccttcaccac aaaccgcccg
     4021 agattcacct caggatgacg gagcccaggg ggaagaatcc gcgcataggc tatgagttga
     4081 ccaccttcat tgcgtccaga aatgtgcctg gcctctggat gtcggtcata ctcatccaat
     4141 tcccgatatg cacattgttg ctccaccaca aacacatcca cccgcaactg caatacgtca
     4201 tacaactgct ggacagtcaa atgctgaaag gtcttcactt cccattgtat gttagccatg
     4261 gcttctgacg agaatttaag gagcgaaatg ggttagttct acggaattgc gggctgaaaa
     4321 acctttggag ttttattgaa tatacttaaa gaggataaat tagaaagggg attccctcag
     4381 tctctcttcc cttctctggc aatccctcat gattcttttc ccctatccaa cttctttcac
     4441 tgcaaaatgg gtcggttact tgcatcaagt atcccttgga ctgcacccaa caaaacagtt
     4501 gatttcacaa atggggaagc aatcttctgt gtccacttca tgactccttg tgtatccccc
     4561 tgaaatgcct gagcgtaggc aattttctgt gaaagatcgg ctagagcttg ctccccctta
     4621 atggaagaaa cgatttctaa cgccctatcc acttttcctt ctttgacata agccaaggag
     4681 acgtccctaa gcgctctagc ccggtgcggt tcatcaatcc ttgaaccaat tcgtacagct
     4741 tcttccaaat ggccattctg aacctgcatg taagaaattt tttcaaacgc cattgctcga
     4801 ttgtaaggag ccatttcgcc tttccattta atcgcatcca aagtccttag cgccccctcg
     4861 atatccccag attcggcttg cccaactgca attgcttcat atggagaata ttccaattca
     4921 gagtccaacg aatcggcaat ctgtaatgct tggttgacct tacctgacct cgcaaggtct
     4981 tgtaatatga aaacatatgg tggaggccca gaaatttctg cattaaagcg agaagcagtc
     5041 tgcaatgctt ctttcacatc tccagctttt gcctgataga ctgcaatttc tcgtaaggca
     5101 tgatttttat gagcaacgat ctcaaccaac tcagttgttt gtaaggcact tgccacgtct
     5161 ccggccttgg cttggcatac cgcaatccgc cacagaatat ctgacttcgc caagctattt
     5221 tgagaggcag atgctccgcc aagggcacgc ttaagattgt cgagaccttc ctgaatcctt
     5281 ccggcttcaa tttgtacaaa agcaatatcc tcccatgcat tggcgctggc atgtccatcc
     5341 ggaattgttt tggcaacctc aatggcaccg tgaatatctt tgctgtctat ttgggccagg
     5401 accactcccc acagggcata ttgccgggaa agttccggcg gaattctgcg aatctccgcc
     5461 aacatttcct ttagatgacc caacttaatc tgttttttgg cgattcccca gagggcctga
     5521 ggatctttat cggctgcctg aagagccgtt tgaataccgc cacgcaggtc tccccttttg
     5581 gcctggatct ctccgatggc ttttaaggca tcggcctttg cattcgttcc catcctaatt
     5641 ctctgtaaaa tcaggagtgc tccagggacg tctccggatt ttgctttctc caaagcgatt
     5701 ccccctaaag tgaactctcg ctcaagactt gaaatgctcg cagctacatt caatgcctct
     5761 gccatcttat ccgtccttgc ttgcgcgaga gaaatctctt ttttcagtct gtttttttga
     5821 taatcatttt gaaacccatc tggaattccc accaatgacc gtgaggcatc gccaatatgt
     5881 ccgacttttg cctgtacaac acccaactct cccgataaaa catatttatc tggaccatcc
     5941 tttagagtgc tcactaaacc agccacctgt ttcagaataa cttgaacatc aaatgattca
     6001 tttatgtcac tggctaagac atgccctgtc gcagaaaaga acaggaggca caccaaacaa
     6061 gaccaattta caactttggc atatctttct tggccctctt tattcatgtt tccaattttc
     6121 atcatgaatt tttcaaaacg gccatttaat ttttggggta taacaaatgt tactcattca
     6181 tcccgcaaca tatggatgaa gaggccatca cgaagtttgg gttcgaacca ggtgcttttg
     6241 ggaggcatga cgcgatccgc atcagccacc gccatcaatt cctcaaccgt tgtcggatac
     6301 aaccagaagg cgacagccca gtccccactg tccaccaacg atacaagttg ttgggttccg
     6361 cgaataccgc ccacaaaatc tatgcgtgga tctgaccgtt ggtctttgat ggctaacaaa
     6421 ggatccagga cacgttccgt caattctgaa acggctagcg cacgcaccgg gtcctgctga
     6481 ctgttcaacg acggatcctg cttgggtttc gcgcgatgcc attgcccctc cagatacata
     6541 tcgaacccgg cggcaggtgg gtcccctggt cgagcgataa tctgcaattc aaagtgtgga
     6601 gacagttttt cgagcaattc ctggggggtg agcccgttca aatcccgaac aacccgattg
     6661 tagggaagga tttgtagttg gtcatgtgga aagatgactg ccagaaagcc ttcttcatgc
     6721 tttccagcag acagtgcgcc tgcctgacca cgggatgcac gaacccgact tgccgcagcc
     6781 gatcgatgat gcccatcggc gatatataag gccgggagat cttgaaagtt tttcatgact
     6841 tcttgaatgg cggatggatc tcgcacgctc cagacggtgt gctcaatctt atcatgagcg
     6901 acaaaatgga catccggctt cgtacccgtc acctctttta accactgatc aattttggcc
     6961 gtctggcgaa aaaagagcaa aaccggcccc gattgcgatt catgggtttc gataatccgc
     7021 acccggtcag tttccttgac cggccgggta tattcatgtt ttttgatgat gccccggtcg
     7081 tattcgtcga cggatgccaa tgccacgatg ccggtctgct catgctgacc ccacttttgt
     7141 cggtagacac cgaaaagtgg ttgtggctct tggaccaaga ccccgtcctt gatgagcttc
     7201 cggagattat cggcccctcg ttgatagacc tcgacagaat atggatcgac tccatcacca
     7261 agctcaagtt cggcccgacc aatgcgaagg aagcaatagg gatttccatc cgctaaatcc
     7321 cgcgcctctt gcaaggagac cacatcatag ggaggggccg ccacttgact ggctaattca
     7381 gatttgggcc tcacagctcg gaagggcgca attgtcgcca tacggtcatt ctccttttta
     7441 aatggatgct cgaaagttga accactgcta atgtccgcgg agccggacct cgcgttcatc
     7501 tgtcgcaagg gtaaacgata ttattgtggc cgggcaacgt attaacgtaa attaccagga
     7561 aaggaattga aaacgagaga cagcagccaa ggagaatcta ctggccgatg taccatccaa
     7621 tactcgagcg ttccaaatag ctcgtcagga gaaagaatga atttgtatag aggagaccgc
     7681 cgactgccac cagaagcaat ccactcatga tggagagcgg tctaatatag ttccggatct
     7741 gccggaaatg ggttaagaac cgatcaagcc atacagcggt gacaagcagg ggaagtccaa
     7801 gcccaagtga gtagaacacc aggagggcca gtccatcagc agcggtatcc gtggtgccgg
     7861 catacaataa gatagacgtg agtataggcc ccacgcaggg ggtccagcct aaggcaaagg
     7921 tgaccccgat gagaaaagaa cccaaatagc ctaccgggtg gctctgaaaa tggagacgcc
     7981 tttcttgaga caagaaacca aaattgaaca gccccaacac atacagacca aacaggacaa
     8041 tcagccctcc tccgatcttt ctcagatggt cctgataatt gatcagccat tggccgaaca
     8101 gactggccga gaggccaaac gcgatgaaga cgactgaaaa tcccccgatg aataacaggg
     8161 aattgacaat gattgtcttc cgaacgtggt gccgacccgt gggagctctg agttggtcaa
     8221 tggaaagtcc cgtgagatat aagacgtggg acggcaccag aggaagcaca caaggggaaa
     8281 cgactgacaa gaggccggct ccaaacgccg ccgacaaggt cacctgagaa atgtgtgtgc
     8341 cggactccac catgagtgtt ctatccctgg cgaatcgatt cgtcccggtt gaacatttat
     8401 tgtttcaaac tcttaagaaa cttctggtac tggggattct ggttgatccc tgcaccacga
     8461 tgcgcaagaa tcatttcccc ctgtgcgtcc atcaccacga atgtcggggt gacattgaca
     8521 ccgaaagcct gcgccacctc attatcctga tcataggcgg tgggaaagat aaaggtgtca
     8581 gggttggctg tgacatactc ctccacatgg gccagactgt cagagaaggc cacagtcaac
     8641 acctggagtt ggtccggttt gcttccccga taaaatttcg aaagaatcgg caattccatt
     8701 tggcaatacc gacaccatgg cgcccaaaag acgagaagcg tcggccgccc ctgtaatgaa
     8761 tttttgctgt acgtatggcc gtcaaatgcc cggagctgaa attccggagc cgggacggat
     8821 gaagccgcag gaaccggcaa ggctccaaaa agcagactca ggccgaccat catgaacccc
     8881 gcaacggcgc ttctgattgc cgagaaccgt cctccccggt ggaagcctgg gagactccca
     8941 ccgaatgtag ctcttttttg gcttgtccat ttataaagct cattccgcat attggctcct
     9001 tacggtttga tatacacata accagcgcgt tgaagatcgg caatccgctt caccgcaacc
     9061 ggatgttcgg tgggtgtgaa cccggcaagt aatttctcca ggggtgtccc atagagcttc
     9121 atggagacct gacacatttc agccttgatc cctttgtcaa tcaccgtctt caatttatct
     9181 tttaactcct gatcgaccgt gtcgctttca aacaatttca gggccggccc atgcacgacc
     9241 agcaccaggt cgatcttgtc tggcccgcct tcagcctcaa tgtgtttatt gatcaatgcc
     9301 aatgcatact tggcgagagc cgggtcgtta ctatctaagt gatacaacac tttcacgttg
     9361 tcctgccccg atatcttatc tgcctgaacc aaaccgggac tcaaaataag tccgtacaca
     9421 caagcaaaca tcagtgtcga gagcatcagg aatgttttag gaatgagatt gggcataata
     9481 gcctccttgg attaaacagt ggatgagaac aggctccgtt tgggagtctt catcgtctga
     9541 ggctattgtg tatgaattga gaggaaagcg gtaggtgcga acacccggag tcgggcgact
     9601 attcttcccg tatgcgaata ggggtaaatg cccctaatgg ggagtattga aaaattgccg
     9661 ccagcggcgt tttcgccgcc tggccgtgct cacgtactcc tccgtacgct ccgctcgccc
     9721 aatcggctgc agccttgctg gacgaacatt tttgaacact cccccaattt ttccggtgta
     9781 agtttcaatc attcataggc tgctgaacag gcaaaacatt tgaaatatcc aacagcccct
     9841 attgggggaa ccggcggacg aactctgccg cagcttgaga tcggcgggtc acttgtaatt
     9901 tctggaaaat attttcgaga taatgaccaa cggttttatc gctcaggtcg agtgctacgg
     9961 caatttcctt attggtttga ccttcggcca ccagtgtcag cacctttaat tcctgtggag
    10021 ctaaggattc tttcttgcca tcggcggaag agattgagag atgctgcatt ttggtgagga
    10081 cccgttgcgt gatcgcagga tcgagaatag actgaccggc agccaccgtg tttaccgcac
    10141 ggactaactc ctccccgctg atttctttca acagaaagcc tttggcccct gccatgatgg
    10201 tcgccatgac cgcctcgtcg tcggcaaagg acgtcaaaaa caacacccgg atgtcgggag
    10261 atgcattccg aatctcacgg caggcgtcga taccgctccc gtcgggcaga cggacatcca
    10321 tgagcaccac atcgggatga agccggtcgg cttcggacac cgccgcggct ctggtgcctg
    10381 cttcacccac gacctgaatg ctctctgttt cagtaaagag cgttttcagg cccaggcgaa
    10441 gcacctcatg atcatcgacc agtaagagac ggatggtgtt tgtcttacgc ctgggcatgg
    10501 ctccctttct tggcaatctc aaaaataact cgagtcccac ggtgaggttt tgaccacacc
    10561 tgaaaatgcg ctcccaggcg cttcgcgcgt gcggccatat tttttaaccc gtgaactccc
    10621 tcatccgaat tctcaggatc aaagcccaga ccatcgtcca taacttccaa gcacacggcc
    10681 ccctcacgca cttgaagcga caccgtgcct gacgtggcgt gtgaatgtcg cacactgttg
    10741 ctgatggatt ctttcacgac atacagcaga tgggtggctt cctccggcgt gagcagatca
    10801 gccgcctcct gatcgatctg cagatggaaa gacaggttct gtgatttttc cacggtctgc
    10861 acaagggtgc gcatctcttc aacccattgc cgtccattca ggcattccgg ttcaagaccg
    10921 ctaatatgtc tacgcaagtc ttgaatcaca aatttgagtt ccatcaagac atcgctgatg
    10981 ccggtgaggg cttcttgagg attctgtgga atgagccgct ggcatcgctc taaattcagc
    11041 tttaaggcaa atgtcgattg aatgacgccg tcatgaagat cctgggccag acgctcccgg
    11101 gcttccaaga ctgtccgaag ctgttgttca ctctgccgac tggcttgttc agcccgtctg
    11161 gtctgaatca atgatgaccg taacttcgag acggtggcca aatatcctcg cccgacatac
    11221 cccagaacga agaaatacac caacaaccgg agtaaatgcc agaaccacca tcgactgccc
    11281 cagatcacgg aataggtaaa catcaattcc gcaaatccga acataagggc caatgaggca
    11341 aacaggatat cttccgatcg acccgaggtt cgataatcca aaaagaagcg aagcgatccg
    11401 gcaaaaaaca gcatgctcgc aaaacttttg ggggcaaccg cggttggcgt gaatacccct
    11461 tcacgaacca tcaccggtac atattgtgga aatccaatcg tccataagcc aagggcaagg
    11521 gctccaccgg tcatcaccca cggcatccat ttaatggttg tatgatgttt tcccgttgca
    11581 gagagccaca tcagcccgaa tcccaaacct cccaacaagc tcgcaaggct ccgcaataat
    11641 acaaatccat tccctggtac tgatatcgcg tgaaagattt caagaatacc catcccgaga
    11701 aagccaccgg cgaggggaaa gaatttctgc ctggtatggt cagtggttcg ttgaagtaaa
    11761 acaattccca tggaaatggc ggccaatcca ccgacagttt ccaatgtgga atggaggggc
    11821 tcatgggccc aacgccaccc cggcaacacc tccggaagga cgacgcccat tatcaacgcg
    11881 gccataccgc atccaccaat gaccagataa atcgagtatg gtttcatagg tccggtttcc
    11941 gcatgtatct tcccagccac cattaagctt tgtctagccg agatggcaca gaccaatcat
    12001 cgtctgttcg atgtgcggtc agagatcaga cacgcgtctc cacatctcaa atgtcaagat
    12061 tgagcatcga tgtgaggtag tggaatttta cgatcttgat gcgttggaca ggtcgaagac
    12121 tgatgtctat tcctgagagt aacagcgcct ggagaagaac aggccgttcc cgccgaattc
    12181 cttatagggc ctgttgaaaa aagcccccag cggcgttctc gccattttcc cgtgctcacg
    12241 tactccgtgt acgctccgcg cgcaaaaatg gctgtggcct tgctggacga cttttttgaa
    12301 ccggcccgaa gcctctgcca ttccagatga tcataggcgt atttgcctat gggtgacctt
    12361 atttttcaac actgtcttaa actctctgtg ggtccaataa acactactca gggaagtgag
    12421 gggtaaggcg tggccatcac gccgcaacga ggttccgact ttcaccttgc gtgtttatcc
    12481 tcccttcaca atccccattt ttttgatacg ggcaaccaac gtcgtgggtt taataccgag
    12541 tcgttcggcc gcgccgcctt ttccataaat tctccattcc gcctgttcaa gggccgccaa
    12601 cgtattttcc cgttcccgaa ggcgcatctc ttggtccgtc atcacactcc atgccatgag
    12661 ggaagcatgg gactcctgag gctgcgaaga agctcgtcct ccccccggct gagggatatc
    12721 gaatgctaat ccctgtcgtt cggaaagaat cagaccccgc tcgatgacgt tttgcagctc
    12781 ccgaacattt ccaggccagt tataacgctg caattgcccc acgagggctt tcgtcagttt
    12841 cggttgcgca cagtggagct tctttgatgt gaccccgaga agatactcgg ttaagagcgg
    12901 aatatcttct ttccgttcgc gaagcggggc aatttcaagc gggaaaacat taaggcgata
    12961 atacagatcc tgccggaagc gaccgttttc cacttcctgt tttaagtcct tgttggtcgc
    13021 agcgatcact ctgacgtcca catgacgagt gcgttcctcg cccacgcgtt caaattgctg
    13081 ctcctgcagt acccgcagga atttgctttg gaggtccagg ggaatttcgc cgacctcatc
    13141 cagaaacaaa gttcccccat ccgccgctcc aaatcgcccg gcccgatctt tgaccgcgcc
    13201 ggtaaatgcc cctttcacgt ggccgaaaaa ctcactttca aacaggtccc gcggaatgga
    13261 ggcacaattc acgcggatta acggccggtc tttccgttga ctgcgtttat gaatttccct
    13321 ggcaaccaat tcttttcctg ttccggattc accgagaatc agcacagtgg cctcggtggg
    13381 tgccacgaga tcaatttgac ggatcacgtt gtgcagggcc gggctttgcc ctacgagatc
    13441 cccgaaggcc ccggcttcga ggacttcttc ctgtaagtac cggttttccg cttgcaagcg
    13501 gttttgccgt tccaggaggg cttctgcctg ttttcgtccg ctaatatcaa ccagaaatcc
    13561 ttgataccac tgcaccgctc cttccgcatt gcggatcacc cgggcatttt ctgaaatcca
    13621 gattggtgtt ccgtccgccc gatagacctg cgactcaaaa tccgtgatga catcctggtg
    13681 ctccagcaac cggcaaaact cctcccgctt cgttggttcg acatagagtt gggaaccgat
    13741 atctttaacg gtttccatca gggcttgcgg ggagtcgtac cccaacaaat gactcagggc
    13801 cgcattggca ttgagaaagg ctccggcagg tgtcgcctgg taaatccctt ccaccgcttg
    13861 attaaaaatg gcgcgatatt tttcttccga tcgctgaagg gcttcttcgg cttcttttcg
    13921 ctgggaaata tctcgaatga ttccgctgaa aaagagccgc tcttcttctt tccaggccga
    13981 aagcgtgagt tcgagaggga attcttctcc ggttcttcgc aaaccactca gttccacggt
    14041 ctctccaaga attcgcgttt ctcccctggt gctgtatcgc tcaagaccgg cttgatgcgc
    14101 ctgtcggtat cgatccggca taagcatggt gaggggctgc ccgacggtct cctcgctttc
    14161 atagccgaag atgtctttgg cccccttatt ccaaaacagg atggtccctg attgatccgc
    14221 gataatgatg gcatcgtgcg aggattgggc tacggcccga aaccgcatct gtgccggata
    14281 actgtccttg gcatcgctca tcggctcgat tgtcccaata ggggtgttga aaaatggaaa
    14341 aaacatgcag aatttgatgt acctctcaca ttaaacaata ttttgctgca atacagcaaa
    14401 atattgttgt aaaacaaaat attgttgaaa tcattttgaa atacatgcaa ttaattactt
    14461 aaaaaacaac catttataca ttaattatgt ttggcacagt atatgctgtt aacattgaca
    14521 ggatatgttg catctattgg caaatatagc caggcattac tcgcattaat cacatttatg
    14581 tctgttggaa aggatggaag aatgaagaat cgacattcag gccggatagg gttgggaatt
    14641 gcgctagtgg caatgaccgg aagtcttttt acatttgcct cacaagtcga ggctaaaaag
    14701 gtgaaggtgg aactcaccgc cgtggaaacg gaagtcgtca ttgacggtaa aggcactacg
    14761 tataaagcat ggacctttaa cggacaggtt ccgggtcccg tcgtccgcgt aactgagggc
    14821 gaccaggtgc atttcatcct caaaaaccac gagaacaata gtcgcccaca ttccatggac
    14881 tttcatgcag cggaaacgga tttcctcgcg aattatcgtg acgtgaaccc tggtgagtct
    14941 ctggaatatg tatgggaagc gaaacggcca ggcgtctttg cctaccactg cggcgcgttt
    15001 ccgatgattc aacatattgc ccgcggcatg ttcggggccg tcattgtgga ccccaaagat
    15061 gcgagtgcaa tgccaaaggc tgaccgggaa tatgttctgg tgcaatcgga gttattcaca
    15121 aaagatcctg atgacgtcgc ggcaatgatg gacgcgaaaa atgaccatgt ggtcttcaac
    15181 ggcgggatat ttaagtatga tcccgttcat gcagcaaagg gaggagagtt tctcaccgcc
    15241 aagccgggtg accgggtacg gttttacttc gtcaacatcg ggccgaataa tttttcctca
    15301 cttcatccga ttgcagaaat ctgggatgat gtctggccca gcggaaatcc ggccaaccgc
    15361 atgcaggggg tgcaaaccca ggtgatcggt gcggcagatg gagccattct ggatgtcgtg
    15421 gtggagcaaa aaggggtcta tccgatcgtg acccactcac tgaaagatgc gctcacggga
    15481 gccattgctc ttcttgaagt aagcgatgaa gccaaggatt tggctctgat gccttcggtg
    15541 ggccccaaag ccatcgacac agccagcaaa tagtcgagga gggaataccc tcaaatctcc
    15601 actacaaccc aacgctcatc tcttcgtggg aggcagattc atcgcctccc acgaagaacc
    15661 gggcttgccc caccatccat tcacgctctt tgagtatttc tgccaaaatg gctgtgacct
    15721 ttatagtaga cctcgagaac cttgcctttt tgccctaaac ccggacaaga gagaacccct
    15781 ttccagtctc agttctccat catctcgagc ctggtatcga actcatgccc gcattggaca
    15841 catcggatac aatcggattc caccaacggt tcgtcatctc ggatccccat tcccccacaa
    15901 tccgggcatc gggctaaatg gaccttttga tcgtcgacgg tttcaaattg cgttccgtgg
    15961 aggcaaaaca ccggttgccc atcaagagcc catggcttcc ccgccttatc aataaccggc
    16021 aggaccagat aatgttgcgc cacataggcc tcctgctcgg ttcgtgtcgg aagaccgtct
    16081 tgaacaagtt tgccggtctt gacctcgtaa agtgcacctt ctttgggaat gacttttgta
    16141 ggtcccatct tcacctcctt tacatgtaaa cacaccgttt taaaaatcag gttgccactt
    16201 gcgacgacaa ctcgagttgt ggtcgtacaa gcgcagcaaa gtcatcgttg acagaccgtc
    16261 acgttcatcc aaaatgcttc tttcaatttt tcgctgaatg aggccgataa cattcacaat
    16321 gaatcacaag aaaatttcgc tagtcatgac tcatcctcat tagggttcat aagcatgccg
    16381 gccttgcccg taagacgttt tttgctggtc atagggtggg tctgtcttgc tctcgggatt
    16441 ttggggctat tttttccact cctgcccacc atgccatttg tcctgcttgc cgcctcgtgc
    16501 ttctccaaaa gttctcctcg cctccattca tggctgctca ggcaacccct gttgggcccg
    16561 atgatacaga attggcacca tgaaggcagt atcaatcaga acacgaaagt cacggcaacg
    16621 gttttcatgc ttggactttt cgggtgctcc ctcctgtttt ttcctttgcc ggctctcctc
    16681 acaatttttc ttgtctgcat tggcaccgga gctttactgt ttatctggac tcgacccctg
    16741 ccgtcaggcc atcttaatca attcagcaat cgccccactg acaccggcaa aaatctggtg
    16801 gggcctggct gatccataca tataggcggg cgtggtgatg actttattac tgtgatccac
    16861 gacatatttt tccaccgcgc aattctgatg tttggcaccg gttttttcta actcagatgc
    16921 ggttccggcg tcctctccaa tggtcacatt gacgcccttt tttcccaaag ccaatgccat
    16981 gatagccggc gcgatacaaa tggccgcgat aggtttatgg ctgtcattaa attctttcac
    17041 gattctgccc acctgtggat caatgtgccc accgctcccc ttttctccaa aatcacagag
    17101 atgcaacgca gctccaaaac ctccgggaaa ggccagggca tcgaaatcct tcgccttgag
    17161 ttgttctaat ggctgaatct cgccgcgagc aatgcgagcg gcctcctgca agacatttcg
    17221 tttttgtccg gtgggttttt gagtgaggtg attcgtctcc tcaacatctt tattcggggc
    17281 aaacacctga tatgccgctc cgttttggcc gattgcaatg agcgtgctaa tggcttcggt
    17341 aatttcagct ccatctaaaa acccacaacc tgaaagaatc acggctactt ttttcatcat
    17401 tttctccttt ccctgaaaac cgaagaaaga acacccttcc caacccggac ggtcccggta
    17461 tttcgtcata agtgttacgc tagtatgact ccgggatgaa ggtcaatctc cagatgtcct
    17521 gaaattctct tttgcttcgg tgcgctccgc ttcttgggat gct
//
LOCUS       DATIAL010000094         8718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_983622, whole genome shotgun sequence.
ACCESSION   DATIAL010000094 DATIAL010000000
VERSION     DATIAL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983622"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VLA60_03170"
     CDS             complement(<1..1226)
                     /locus_tag="VLA60_03170"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSF08386.1"
                     /translation="MGTNNRGSRGWQFQWPLCFTRMNTRKAGLAGIGFLAGILLLGSW
                     NMPFHSQAAISQPADPASVGSPPNMDKGGFADIAQAVTPAVVNITVRKEAPVPMSGMP
                     SDPMREFFGLPGMPEMPGMPKRPDGPPAPEGQGAGSGVIISSDGYVLTNDHVVDGAKE
                     IMVTLPDNREFTGKVIGLDPQTDLAVIKIDGKDLPYVPWGNSGELRVGEYVLAVGNPF
                     GLNSTVTLGIVSALGRGGMGITQYEDFIQTDAAINPGNSGGALVNTRGQLVGINTAIF
                     SQSGGYQGVGFAVPTNLAKPVYDSLVSTGKVVRGFLGVGIQAVTTDLADSFKLDQSKG
                     ALVTNVVPGSPADKAGLKRGDVIVEYQGKPVLDPRNLQHHVIRTTIGTEVKMVVIRDG
                     QKHTLKAEIREQDKPV"
     gene            1807..2130
                     /locus_tag="VLA60_03175"
     CDS             1807..2130
                     /locus_tag="VLA60_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08387.1"
                     /translation="MEILALVIAIVALVIAIMAFQRTGGGMRDLRRQIEDLTEKSEQA
                     TKGARDMTADALSRLETFIRGQQKPSPAQQEPLKDEPLTTQQTSAPTEPEKPKDEPPP
                     TEQRP"
     gene            2127..2567
                     /locus_tag="VLA60_03180"
     CDS             2127..2567
                     /locus_tag="VLA60_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08388.1"
                     /translation="MRPWVIPATVFFLGLGLGLSSPMLASRYLDSYFPAFLKDAMHPL
                     EGSVAHKQRQEDRLLVTITTQDGSILATFQQQVPEIDLLIEERDLVTLDVQKYKPFIH
                     DPTLLRVTKHKEMNPTIAAPPNPSMPSAPTKEPGSTIDYSGPVP"
     gene            2690..3289
                     /locus_tag="VLA60_03185"
     CDS             2690..3289
                     /locus_tag="VLA60_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08389.1"
                     /translation="MLGILQVLLLVGMLTACGAMNAIPPPASPTAVQQFLMAQAVERS
                     LYGENTMPIPLTSGDSVSLDISGLSVEQGLAVAQDFFKGAIEGWLGERGLKIALDPTK
                     AKYRIHILVQSLGVEQRSSLFGIPPIQSTLLPIALPEMAIYSSQKLSGYTRFRLDIYE
                     TATGQFVRSTPWFQGSTYFNEYTILFFIDFEFTNLIAPF"
     gene            complement(3441..4931)
                     /locus_tag="VLA60_03190"
     CDS             complement(3441..4931)
                     /locus_tag="VLA60_03190"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSF08390.1"
                     /translation="MGIKQCKVSRHFPRLTAILSSGLAMWIGCGGVVALSIGMSISAG
                     WAGESSGSSLERTEEKTFKQGGFADVAQAVTPAVVNITATVDIVESRGGGIPLPFRGF
                     GEGQPPPGIPKRQKGSGSGVILSSDGYIMTNNHVIAQSRDLLVTLPDAREFPAKVIGA
                     DPETDLAVIKIQADKLPVIPWGNSASLAVGQYVLAIGNPFGLNSTVTLGIVSALDRGG
                     MGLARFEDFIQTDAAINPGNSGGALVNTRGELVGINTAIASQSGGYQGVGFAVPSSLA
                     RPVLEELIKHGKIIRGYLGLGVQEMTTELAPWFGLKSGEGALVTDVVPGGPADKAGIH
                     RGEVILEYQGKTVKDGQMLLEQVTRAPIGEQIELGMVENGKERKVRAVIREQPPIPQA
                     GRTRTPRVEHPLAGVEVADVDEAAVQEYGLNPVTKGAVVAFLEEGCAAELAGIIEGDV
                     IIELNKHPVESVREYSSWFKRLKKEQAVLVVLVRSSRHLYVVVKSS"
     gene            complement(5448..6215)
                     /locus_tag="VLA60_03195"
     CDS             complement(5448..6215)
                     /locus_tag="VLA60_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF08391.1"
                     /translation="MGSQKTSKTDRVGTLDQDITRIREQLEILKKFLIDEPSTVALNE
                     FDSATEDILADALGSSSPLLDTYDYAQLGEAAGLMNLSEEAPEGTTHQSQRETIRQRQ
                     RVLESAIADLEARRASLSQVSKKRKASGPSVSDYMAKTVRSVSLDATLQEAGQCLQKW
                     KIGSVLIQSGDEYLGYITETELTREVVASGTDPVTTTVKTCMREPLVTVETSDPIVEA
                     VRLMKEKGTRHLAVTESSRIVGVVSVSDIIRYYSGVM"
     gene            complement(6389..7696)
                     /gene="mgtE"
                     /locus_tag="VLA60_03200"
     CDS             complement(6389..7696)
                     /gene="mgtE"
                     /locus_tag="VLA60_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSF08392.1"
                     /translation="MNVRARGHLLNEAFFQNHHEEAARILEGYPVEDILRVLQGTSPK
                     NAANLLACVTPPVAADTLAIMPTDLLKQVVPHLSPSLAASLFQRLDDDLQRAILFRVP
                     ERYAQEIRSYMTYPEESVGRIMDPKFFVLQEESTVREAMVQVRIRAQKDVHDVYVIDR
                     TQKLVGRISVRDLLLVDPEEKLQSVMVQDLPTIHPLESREEIVELFGERHFFTIPVVD
                     LDERLLGVVRNEAIIKASQEDASADLLTMVGANKDERALSPVWFSVRKRLPWLQLNLL
                     TAFLAAFVVGMFEGTIAKFTALAVLLPIVAGQSGNTGAQSLAVVIRGLALRDIRPSQW
                     LRVSGKEVYVAFLNGLAVSATACLAVGLWSRSLGLVFIIGLSMIISMVIAGLSGAIIP
                     IALKALKQDPAQSSSIVLTTVTDVVGFFSFLGLATLLSSLLET"
     gene            complement(7720..8556)
                     /locus_tag="VLA60_03205"
     CDS             complement(7720..8556)
                     /locus_tag="VLA60_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF08393.1"
                     /translation="MELEHRLTLGYLQAHPVEAARHLESLDPHEAASLLEALPGEEIA
                     GVLEHCLPVSTAKIIEELSNDLGAGVLGAMNATSAIGVLRQFEEPVRQDVLDRLDNPM
                     GATLRRALRYSPNTAGSLADPQVFTLPPDIAVEEAIDRIRTYGQKAMYYVYVIDRDSK
                     LEGVLTLRQLLIDRSDHLVGTLMETQIITLSAEANLEEILKHSEWSRFHTLPVVDRWG
                     TFFGALRYRMLRRIEKDVGGKAPLGSVSDSLMQLWEVYSLTGIRLMTDVAETLQERNK
                     IG"
BASE COUNT         2217 a         2435 c         2024 g         2042 t
ORIGIN      
        1 tggacaggct tgtcttgttc acgaatctcc gccttcaggg tatgcttctg accgtcccgg
       61 attaccacca tcttcacttc cgttccgata gtggtcctga tgacatgatg ctgaagattc
      121 cgcggatcca atacgggttt gccctgatat tcgacaatca catcaccccg tttgaggcct
      181 gccttgtccg caggacttcc aggaacaaca ttcgtgacca gggcaccttt ggattgatcc
      241 aatttaaacg agtctgcgag atcggtcgtg acggcttgta taccgacgcc caaaaatcct
      301 cgcacaactt ttccggtact gaccagactg tcatacaccg gtttggcaag attggtggga
      361 actgcaaatc ccactccttg gtacccgccg gattgggaaa agattgcggt gttaattccg
      421 accagttggc ctcgcgtatt caccaatgca ccaccggaat tccccggatt gatcgccgca
      481 tcggtttgaa taaaatcttc atactgagtg atgcccatgc ctcctcgacc taatgcgctg
      541 acaatcccca gcgtgacggt ggaattcagt ccaaaaggat tgccgaccgc caggacatat
      601 tctccgactc gaagctcgcc cgaatttccc catggaacat acgggagatc cttcccgtca
      661 attttaatga cggctaaatc agtctgggga tcaaggccga tcacttttcc ggtgaattcc
      721 cgattatccg gaagggtcac catgatttcc ttggccccat cgaccacatg gtcgttggtc
      781 aacacatatc catcactaga gataatgaca ccggaaccgg ctccctgacc ttccggagca
      841 ggaggcccat ccggcctttt cggcatgcct ggcatctccg gcattcctgg cagaccaaaa
      901 aactctctca tcggatcaga cggcatcccc gacatgggga ctggcgcttc ttttcgaacc
      961 gtaatattaa ccactgcggg ggtcaccgcc tgggcaatgt cagcaaatcc tcccttatcc
     1021 atgtttgggg gagatcccac ggatgcagga tctgccggtt gggaaattgc tgcttgagaa
     1081 tgaaacggca tattccatga cccgagcaga aggattcctg ccaggaaacc gatcccggcc
     1141 agtcccgcct tccgggtatt cattctggta aaacacaacg gccattgaaa ctgccaacca
     1201 cgagaaccac gattgttagt acccattgac attcctcctt gatagttaga aagccgacac
     1261 tgagatattt cgtatctgca tcctatccat tcagaataag ctgaggatta actgactatt
     1321 aattttgatt aattttcaca cgattcgctg atgggtctgt ccatatgttg gccgtctcaa
     1381 ggagttccgt tcgataaggg caacaggcac atcaacacaa caggtaatcc tgactcactt
     1441 tttcacgaag aaatcaaatg ccggcggcaa cgccatcgcc aacagaaagg cgcctctgcg
     1501 ctcatgtcat ccggaggccg gggttagcca tgttcccgat ttgcgcaaac agacctcgga
     1561 cccatccatg agaagcgtca acatctacct atcaagttgt ccgtgtacag acaaaaacaa
     1621 gttcacggta gattcccaat gaatgacgtg tcacaataat gaaaggaggg aagacagact
     1681 ggaatccgga aggacaagag gacggtcatt tcagcatagg tgcctcaatc tgcaaattgg
     1741 tacttacaag accgccaacg cttttcacat ttcaaaagga cggcttgacc ggaacaggag
     1801 gacatcatgg aaatcttggc cctagtgatc gctatcgtcg ccttagtgat tgcgatcatg
     1861 gcatttcaac gaaccggcgg gggaatgcgc gatctccgtc gacaaattga agacctcacc
     1921 gaaaaatccg aacaggcgac caagggggcc cgcgacatga ccgctgatgc cctcagtcgc
     1981 ctggaaacgt ttattcgtgg acagcagaag ccttctcccg cgcagcagga acccctgaag
     2041 gacgaacctc taaccacaca gcaaacctct gctccaacag aaccggaaaa accaaaagac
     2101 gaacctccac ccacggaaca gagaccatga gaccatgggt cataccggcg acggtatttt
     2161 ttctaggatt aggccttggc ttaagtagcc cgatgttagc ctcacggtat ttggattcct
     2221 attttcccgc atttttgaag gacgccatgc atccacttga ggggtcggtg gcacacaaac
     2281 aacgccaaga ggatcgttta ctggtcacca ttacaactca agacgggagc attttagcca
     2341 cctttcaaca acaggtgccg gagatcgacc tccttattga ggaacgagat ctggtgacat
     2401 tagacgtgca gaaatacaaa ccttttattc acgaccccac attgcttcgg gtcaccaaac
     2461 acaaggagat gaaccctacc attgcggcac ctccgaatcc ttccatgcca tctgcaccga
     2521 caaaggaacc cggctcaacg attgattact caggacctgt cccctagact gatgaggaag
     2581 gactgagcgc gagctcgcct tcacatggac ccgggtattt ttcctgtctc atcaaaaatt
     2641 agaggtgaaa gtggtttggt taaccgaagc acgtccgtgg aataggtcca tgttaggtat
     2701 tctacaagtt ctcctgttag tgggaatgct gaccgcctgc ggcgccatga atgcaatccc
     2761 cccacccgca tctccaactg cagtccaaca attcctcatg gctcaggctg tggagcgcag
     2821 cctatacgga gagaacacca tgcccattcc cttaacctct ggagattccg taagtctgga
     2881 tatctctgga ctaagcgttg agcaaggatt ggcggttgca caagattttt ttaagggcgc
     2941 gattgagggg tggctgggag aaagagggtt gaaaattgcc ttggatccga ctaaagccaa
     3001 gtatcggatc cacatccttg ttcaaagtct aggagtcgag caacggtcct ccttgttcgg
     3061 gatacctcca atacagagca cgctcctccc aattgcatta cccgagatgg caatatacag
     3121 ttctcaaaaa ttgtcaggat atacccgctt ccgcctggat atttatgaaa cggcgacggg
     3181 acaatttgtt cgctccactc cctggttcca agggtcaacc tacttcaacg aatatactat
     3241 tttgtttttc attgatttcg aatttacgaa tctgatcgct ccattttaat ccggtgctct
     3301 tatttgcaac caggaacctt caaacatcca cggcctcttg ggaaaacaca aggaagcatc
     3361 gctcaaaacg tgtaaatgcc agacaacagt tcgtctgaag aatccgtgaa tcgccggaaa
     3421 cccttctcat cctgactcat tcaggaggat tttaccacca catacaaatg acgggaggaa
     3481 cggacaagca caaccagtac cgcttgttcc ttcttcagcc gtttaaacca tgaggaatac
     3541 tcccggacgg attccacggg atgcttattc aattcaataa tgacatcgcc ttctatgatc
     3601 cccgctaatt cagcagcaca tccttcctca aggaatgcga ccaccgcgcc tttggtgaca
     3661 ggattcagcc catattcctg aacggccgcc tcatcgacat cggccacttc cactccggca
     3721 aggggatgtt ccacgcgagg cgttcgagta cgccccgcct gtgggatcgg tggttgctca
     3781 cgaatgaccg ctctgacctt acgttctttt ccattttcga ccatccccaa ttcaatctgt
     3841 tctccgatgg gagcccgcgt cacttgttcc aacaacattt gtccatcttt cacggttttt
     3901 ccttggtact caagaatcac ctctccccgg tgtatgccgg ctttatcggc cggcccacca
     3961 ggaacgacat cggtcactag ggccccttct ccgcttttca ggccaaacca cggagccagt
     4021 tccgtcgtca tttcctgtac tcccagccct aaatagcccc tgataatctt gccatgtttg
     4081 atcaattcct ctaacacagg ccttgccaat gaagagggca cagcgaatcc gactccttga
     4141 taccctccgc tttgagaggc tattgccgta ttgatgccga ctaattctcc tcgggtgttc
     4201 accaatgccc cacctgaatt tcctggatta attgccgcat cggtctgaat gaaatcttca
     4261 aagcgggcta accccatgcc tcctcgatca agagcgctga cgatacctag cgtcaccgtt
     4321 gaattcagac caaacgggtt gccaatggcc aagacatatt ggccaacggc taaggaggcg
     4381 gaatttcccc agggaatcac aggaagtttg tctgcctgaa ttttgataac cgccaaatcc
     4441 gtttcgggat ccgccccaat aaccttggca ggaaattcac gtgcatcggg aagagtgaca
     4501 aggagatcac ggctttgggc aatcacatgg ttattggtca taatatatcc gtcggaagac
     4561 agaatgacgc ctgaccctga acccttttga cgtttgggaa tacccggtgg cggttggccc
     4621 tcaccaaaac ctctaaacgg aagtggaatc cctcctcccc gggattcgac aatatcaacg
     4681 gttgccgtga tattgaccac agccggggtc acggcctgag ccacgtccgc aaatcctcct
     4741 tgcttgaatg tcttttcctc cgttcgttcc agggaggacc ctgaggactc acctgcccat
     4801 ccggcactta tgctcattcc gatactcaga gcaaccacac cgccacaccc gatccacatc
     4861 gccaggccgg aactcaagat cgccgtaagc ctgggaaagt gacgcgagac cttgcattgc
     4921 ttaataccca ttgttatccc catgatcggt tcttgatgta tggtctgcga tttcggaata
     4981 aaatatggac ctgttgaaaa aatccgccag cggcgttccc tgccttcgcc ggagcggctt
     5041 cgcgcaggca ggtcgccatt tttccgtgct cacgtactaa gcgtacgctc cgcgcgaaaa
     5101 aacggctgcg gccttgctgg actgactttt ttgaaccgac ccggagcctt tgatgagtaa
     5161 tcaaatcttg ggcaaattgg cccttgaaaa tctttattat tcaacactcc caatatgcga
     5221 ttgactaaca acacgaatgt ccccagaggt ccatcaggca gatttttctc agcttaggtc
     5281 cgctccctcc gaaagcttca ttcaatggcg gatcaaatat ttggtggaaa aatgtggaac
     5341 cttcctcgag aaacacgacc acgaaaaaac ccgggtgtgt ccccgaggaa gagagcctga
     5401 tagcccgagg ctagaaggcg agtgataatc tctcagaaga ctaatgttta cataactcca
     5461 gaataatacc ggattatatc tgaaacggag accacaccga cgatcctgct actttctgtg
     5521 accgcaaggt gcctggtacc tttttctttc atcagacgca cggcttcgac aattggatca
     5581 ctggtctcaa ccgtgactaa cggttcgcgc atgcaggtct tcaccgtggt ggtcacaggg
     5641 tctgttccgg atgcaacaac ctctcgagtc aattcggttt cggtaatata ccccaaatac
     5701 tcatctccgc tttgaattaa caccgaccca attttccatt tttgaaggca ctgcccggct
     5761 tcctgcaagg tggcatcaag cgacacagat cgcacagttt ttgccatata gtcggaaacg
     5821 ctcggcccgg acgctttccg ttttttggaa acctgcgaca gggaggctcg ccgggcttca
     5881 agatccgcaa tcgcgctttc cagcactcgc tgccgttgcc ggatcgtttc ccgctgtgat
     5941 tggtgggtgg tgccttccgg tgcctcttca gaaagattca tcaaccccgc tgcctctcca
     6001 agttgggcat aatcataggt atctaagagc ggagaggaac tgcctaacgc atcggcgaga
     6061 atgtcttctg tagcggaatc aaattcgttt aaagcgaccg tggaaggctc atctatcaga
     6121 aattttttca agatttctaa ctgttcacgt atccgggtaa tatcctgatc caatgtcccc
     6181 acccgatccg ttttagacgt cttctgtgat cccatagcgc ctcctttcct caacgacata
     6241 agataaaaat gagagcaaaa acgatactac cctaccatca attagtaggc aatcagaaat
     6301 tttcttggaa ggaaaagtgg cccgcgaggg gtatgggagc gggatggagc ggacatgaag
     6361 aggatcctgt ccacaaaccg atcaatgatc aggtttccaa taaacttgaa agcaaggtgg
     6421 caagccccaa gaaactgaaa aacccaacca catcggtcac cgtggttaac acaatggagg
     6481 aggattgagc cggatcctgt ttgagtgcct tcaaggcgat gggaatgatc gcaccggata
     6541 accctgcaat gaccatcgaa ataatcatgg acaagccgat gatgaagacc aatcccaacg
     6601 accggctcca taatcccacg gcgagacagg ccgtggcaga aacagccagg ccgtttaaaa
     6661 atgcgacata gacttctttc ccactaaccc tgagccattg ggatggtcgt atatcccgca
     6721 gtgccaatcc gcgaatgacc acggcaaggg attgggcccc ggtattgcct gactgcccgg
     6781 cgacaatcgg gagtaagaca gccagagcgg taaatttcgc tatggtgcct tcaaacattc
     6841 ccacgacgaa tgcggccaag aatgccgtta acagattcag ttgtaaccac ggcaatcgtt
     6901 ttcggaccga aaaccacacg ggggaaagcg cacgctcatc cttattggct cccaccatcg
     6961 taagaaggtc tgcactcgca tcttcctgac tggccttaat aatggcttcg ttccggacca
     7021 cgcctaagag ccgttcgtcc agatccacaa ccggaatcgt aaaaaaatgc cgttccccaa
     7081 agagttcgac aatctcttca cgcgactcca aaggatgaat ggtgggaaga tcctgaacca
     7141 taaccgactg caatttttct tccggatcca ccagcaacaa gtcacggacg gaaatcctgc
     7201 ccacgagctt ttgggttcga tcaatgacat agacatcatg gacatctttc tgcgccctga
     7261 tccgcacctg taccatggct tcccgaaccg tgctttcctc ctgcaacaca aaaaatttgg
     7321 gatccataat acgcccgacg gattcctccg ggtaggtcat ataggaccgg atctcctggg
     7381 cataccgttc aggaactctg aaaagaatcg cccgttgcag atcgtcgtcc aaccgttgaa
     7441 acagggatgc agctaatgac ggcgagagat gcggcacaac ctgtttcagc aaatcggtgg
     7501 gcatgatcgc caacgtatcc gcagccacgg gaggagtaac acaggccaga agattcgccg
     7561 catttttcgg actggttcct tgcaagactc gcagaatatc ctccaccgga tacccttcca
     7621 gaatgcgtgc ggcttcctca tgatggttct gaaaaaatgc ctcgttgagg agatgacccc
     7681 ttgctcgaac gttcatactc ctctgcgtgg tcagatccct caccctattt tgttccgttc
     7741 ctgtagcgtt tccgctacat cggtcattaa gcgaatgccg gttaacgaat agacttccca
     7801 aagttgcatg agcgaatcac tcaccgatcc caacggagcc ttgcctccga catctttctc
     7861 aatcctgcgt aacatccgat agcgcaaggc tccaaaaaat gttccccatc gatcgaccac
     7921 aggcaaggta tgaaatcggc tccactcaga atgtttgaga atttcttcca gattggcttc
     7981 tgctgataag gtgatgatct gggtctccat caatgttccg actaaatgat cagaccgatc
     8041 aatcagcagc tgcctcaatg tgagaacacc ttctaattta ctatcgcgat cgatgacgta
     8101 gacatagtac atggctttct ggccataggt acgaatacgg tcgatggcct cttccacggc
     8161 aatatcaggt gggagggtaa acacctgagg atccgctaaa cttccggccg tgttgggtga
     8221 atatcgtaag gcacggcgaa gggtagcccc cattggatta tccaaccggt ccaacacgtc
     8281 ctggcgaaca ggttcttcaa attgccgaag aaccccgatt gctgatgtgg cattcatggc
     8341 gcctaagaca cctgctccaa gatcattgga aagctcttcg atgattttgg ccgtggacac
     8401 cggcaaacaa tgctctaaga caccagcgat ttcttcccca ggtaaagcct ccaggagcga
     8461 agccgcttca tgaggatcca gcgattccaa atgacgagcc gcttccacgg gatgggcctg
     8521 caaatacccc agagtcagcc gatgttcgag ttccatatgc taatgctcgg gatgggtaat
     8581 gacggctgtt gattcggtaa cctgggtcgc aggaacaatg acccggccct cacgggtttc
     8641 taacacgacc gagatgggtg tgaccgcaac cacggttcct tggttctcgc caatttttac
     8701 ggtcatgccc actcgaac
//
LOCUS       DATIAL010000095         3977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_123979, whole genome shotgun sequence.
ACCESSION   DATIAL010000095 DATIAL010000000
VERSION     DATIAL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123979"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VLA60_03210"
     CDS             <1..1087
                     /locus_tag="VLA60_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08394.1"
                     /translation="GSEEHHVVSDSGTEKSVGHAIAGAPFQIYLSEEVGKDEQGSREQ
                     ARADDALQTVIGAFNYMMEHRTDYARFQEALAKDALQKVIIEPKVANREGKEFLLLVA
                     RTSQPNRVNLLISASGLEEQGYLNHPEQLVPVLAREFQWVVSKSDTTPKRQAKVLPSD
                     LTQAPIKTNQEIAELSGEDREHLLQALFRTYLTTTDRHNSLEGQPYYETGSTTRIPPA
                     QPDSTTKLYDIRVREALQKIVREPDFQEQTPKAVRSLLNGKIWNVAFADIHNRDWATR
                     TRVVPKEQSITAGEQDTTIQPAMVLINIHRSASPDDPLFEETRGLPMGALPAEILARV
                     IAREIQDNITEKSMRGHVAQDELSAPQ"
     gene            1409..1612
                     /locus_tag="VLA60_03215"
     CDS             1409..1612
                     /locus_tag="VLA60_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF08395.1"
                     /translation="MIQEIVAKIREGMNIRNDLLAGLVVALALIPEAMAFSIIAGLDR
                     GADKNLRRPWIGHLHGGRHGPTS"
     gene            2423..3433
                     /locus_tag="VLA60_03220"
     CDS             2423..3433
                     /locus_tag="VLA60_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08396.1"
                     /translation="MVKKIAATFGEGIKNASDIGYHIVILCLSAGIALSLTTVARSFL
                     TYWTRVEKEKMSIVALEIGVAALLIVVMTYIRRSWQDRKLAKMAIDAGFVSFFPKRAR
                     RAEDGIRKLRQTQGRGRTVLVIGSSGYDTLRDQVGDLSTILDKCLGANIMLVNPFSQG
                     ASARIRAIADPMMSIEKYRAGVRQSIELLKRLQGVGKSVKLKLYSDPPLLKLVILGDY
                     LWMQHYHTDLDIQEMPEYVLRHNANDHGLYTLCYQYFMQRWEQTDIPEYDLVTDELVY
                     RDKNGREIRREQFWPDDIPQSIEQGKEIPLLPSTPHTPRERPALEHVMCRTATEVVRS
                     HF"
     gene            complement(3698..>3977)
                     /locus_tag="VLA60_03225"
     CDS             complement(3698..>3977)
                     /locus_tag="VLA60_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08397.1"
                     /translation="VKNISDAVKFVRDAICHVDSDNRNYEECNARLSYNIAYGKSNLA
                     KIGDVEIRSDFEDDVCFFFGSQKLYLRRHIRRAYEEAKGKLLPLVEKA"
BASE COUNT          972 a          983 c         1085 g          937 t
ORIGIN      
        1 aggttccgaa gagcaccatg tggtttcgga ttccggaacc gaaaaatccg tgggacatgc
       61 cattgcgggt gcgccgtttc agatttatct ttccgaggag gtcgggaagg atgaacaagg
      121 cagccgggaa caagccagag ccgatgacgc gctccagacg gttattgggg cgttcaatta
      181 catgatggaa catcggactg actatgccag atttcaagaa gccctggcca aagatgccct
      241 gcaaaaagtc atcattgaac caaaggtcgc gaaccgggag ggcaaagagt ttttgttact
      301 ggtggcccgc acctcacagc ccaaccgggt caatctcctc atcagcgcat ccgggcttga
      361 agaacaaggc tatctcaatc atccggaaca actggtgccg gtcctggcca gagaatttca
      421 atgggtggtg agcaaaagcg acacgacgcc gaaacgtcag gcgaaggtgc tccccagcga
      481 cctcacgcag gccccgatca agaccaatca ggagattgca gaattgtcag gagaggaccg
      541 tgaacatttg cttcaagcat tgtttcgcac ctatttaacc actaccgacc ggcataatag
      601 tttggagggg caaccctatt atgaaaccgg ttccaccaca cggattcccc cggcgcaacc
      661 cgattccacc accaagttgt atgacattcg ggtgcgtgaa gccctgcaaa aaattgtgcg
      721 ggaacctgac tttcaggaac agactccgaa ggcggtgaga agtctgctta atggcaaaat
      781 ctggaacgtc gcgttcgcgg acattcataa tcgggattgg gccacgcgaa cccgggtggt
      841 ccccaaagag cagtccatca ccgcaggcga acaggacaca accattcaac cggccatggt
      901 gttgatcaat attcaccgat cggcctcgcc tgatgatcct ttgtttgaag agacgagagg
      961 gctccccatg ggtgctctgc cggctgagat cttggcacgg gtcatcgccc gggaaatcca
     1021 ggacaatatt acggagaagt ccatgcgtgg ccatgtagcc caggatgaac tctccgcccc
     1081 tcagtgatct ggaggcgtgt tcgccatctt tcttttccca cccttccaat catgccgctt
     1141 gtgcgtgtga agagtctgtg atattgtcgg atgcatcgtg taagacctct tcttccgggg
     1201 cgacagaccc cttaaaagcg aagcaaggcc ttacacgatt ttttttgttc gaaccctcct
     1261 tggtctcctg aaagcaggaa cattccaaca agcatccggg agttgtgtga tgggacagaa
     1321 agggtgtcgg caggtgccgt tactcctagg gccccttggc gtgtgttttt gagaagcatg
     1381 tctgtctgaa tcaaaacaag gatcattgat gatacaagag atagtggcca aaatccgtga
     1441 agggatgaat atccggaacg atctgcttgc aggtcttgtc gtcgcactgg cactcatccc
     1501 cgaagcgatg gccttttcca ttattgccgg gcttgatcgt ggtgctgaca agaatctccg
     1561 tcgtccttgg attggacatt tgcacggtgg gcgacatggg ccaacttcct gaccgccttc
     1621 ccatattcct gctcccggat gtgccgttga actgggaagc cttgtggatc atttttccgg
     1681 tgtcagccac cctcgcgtgg tgggactgtt ggaatccatg attcgcctta ttgcggtccg
     1741 ctaaagttcc gacgctgttg ctccgataga gtatctggcg ttacttcgtt ttaatctggt
     1801 accaccaaat taaaaaaagt gaaaacagag tggctgtgac tggttgtcgc cacttgcagc
     1861 ttcggtagac gagccgggga atctcagtga tgttcaacat tcgaaggggt gatcgtagaa
     1921 gaaaaaataa taagccaggc atgcacatgc ctcgcttgtc ggattgaaaa cggtgtgata
     1981 ccgtaaagcc tatcgtgtaa gacctctttt cccggggcga gagacccctc gaaatggaaa
     2041 cagagactta cacgattttt ttgtgcaaac cctccgtggt ctcctgaaag caggaacaat
     2101 cccacaagga tccaggagtt gtgtaatagg gagtgttgaa aaatacggtt tcccaaaggc
     2161 aaatacgccc atgatcattt gggattccag tgtctcgggc cggttcaaaa aagtccgtcc
     2221 agcaaggcca cagtcatttt tgcgagcgga gcgtacacga agtacgtgag caaggaaaaa
     2281 tggcgcgaac gctgctggcg gcttttttca gcaggccctg atagggcagc aatggttgtc
     2341 ggagtgggct gatacctgaa tctgttaaat cgtatgtgta aattatttgt agttttctcg
     2401 tgaaaagaaa gaaggaaaag tgatggtaaa aaagattgcg gccacattcg gtgagggtat
     2461 caagaatgcc tccgacattg gctatcatat tgtgatcctg tgcctgagtg cggggattgc
     2521 gttgtcgctc actaccgtgg ccagaagttt tctgacctat tggacaaggg tggagaagga
     2581 aaaaatgtcg atcgtggcct tggaaatagg tgtcgcggcg ttactgattg tggtgatgac
     2641 ctatatccgg cggagttggc aggatcggaa gctggccaaa atggcgatcg atgccggctt
     2701 cgtatcattt ttccccaaac gggcgcggcg ggctgaggat gggattcgca aacttcggca
     2761 gacccagggg cgtggccgga ccgtcctggt catcgggtca agcgggtatg acaccttgcg
     2821 tgatcaggtg ggggacttat cgaccattct ggataaatgc ctgggagcca acatcatgct
     2881 ggtgaatccc tttagccagg gtgccagtgc acggatccgg gcgattgcgg atccgatgat
     2941 gtcgattgag aaatatcgag cgggcgtcag gcaaagcatt gagttgctca aaaggcttca
     3001 gggggtcgga aaatccgtca aactgaagct ctattcggat cctccccttc tgaaattggt
     3061 cattcttgga gactacctct ggatgcagca ttaccatacc gatctggata ttcaggagat
     3121 gccggaatac gtattgcggc acaacgcaaa cgatcacgga ctgtatacgt tgtgttatca
     3181 atatttcatg cagcgttggg agcagaccga cataccggaa tacgatctgg ttaccgatga
     3241 gctggtctat cgggataaaa atggccggga aatcagaaga gaacaattct ggcccgatga
     3301 catcccgcag agcatcgagc agggaaaaga aatccccctg ttgccctcaa cgccacacac
     3361 gcccagggag cgtcccgctc tggagcatgt catgtgtcga acggcgactg aagttgtacg
     3421 atcgcatttc tgaccgtctg aataggatat ctcccagcta cttttcagca aggctcggac
     3481 aaaaaacctc atttcctttc gtccgacgcc tgtaggttgt catgggacct gcgtgtcgtc
     3541 ggcgcccagt aaggcctagg ctggcgcggg tcatgatcgc ggaggaactc gtagataaat
     3601 ccgaagcctt gtgacgcaag ttcttgtgca atttaacgtt gatggtcagg ggcgacaacg
     3661 aagcgcaggg tagttgccgc ccccgttgac caccttatta tgccttttcc actaatggca
     3721 ataatttccc cttcgcttct tcgtaagctc tgcgtatgtg ccgccggagg tagagcttct
     3781 ggctcccgaa gaagaaacaa acatcgtctt cgaagtctga cctgatctcg acgtctccga
     3841 ttttagcgag attactcttg ccgtatgcga tgttgtaact taggcgcgca ttacactctt
     3901 catagtttcg attgtccgag tcaacgtggc aaatcgcgtc ccgcacaaat ttcacagcat
     3961 cggaaatatt cttcact
//
LOCUS       DATIAL010000096         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_553968, whole genome shotgun sequence.
ACCESSION   DATIAL010000096 DATIAL010000000
VERSION     DATIAL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553968"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VLA60_03230"
     CDS             <1..826
                     /locus_tag="VLA60_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSF08398.1"
                     /translation="NTRDEPHAHQADYRRLHVIVGDANMSEISTFLKVGTTAIIMEML
                     EQQGPLPHIELTDPVKAIKAVSRDLTVKSSLPLNNGQHTTALAIQRAFLQSAHDFYRT
                     REVSPVTKEVLVRWESTLDTLERDPFELRREVDWVAKHALIQSYMDRKHCSWNDPRVA
                     MMDLQYHDVRPNKGLYYTLERTGQIERLTLELEVERARQTAPAFTRAFFRGQCLKHLP
                     GQVYGMSWTSVLLHTGNSTIKRIPLMDPYRGTQQLTQELFRDITSVDQLLSRLVKS"
     gene            869..1711
                     /gene="prcB"
                     /locus_tag="VLA60_03235"
     CDS             869..1711
                     /gene="prcB"
                     /locus_tag="VLA60_03235"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HSF08399.1"
                     /translation="MNSENVSLPNFSADPCSSFYQYLTSQRPDLLPMARWENSLPQPG
                     PTDPSLISNRHLPWPHGTTVLAFTYDNGVLIAGDRRATEGFQIAANRMEKVFQTDGFS
                     AMAIAGAAGPCVEMAKLFRIELEHYEKLDGQPLTCEGKANRLGHMVKANFPMVLHGLV
                     VIPLFVGYDHKREIGRLFKYDITGGRYEDTDFHAVGSGGKDARSTLKEYFRKNLPEDA
                     AVKVALRALLNAADEDVGTGGPDLVRRIFPTVKLVDSRGVRDVDDTQLATVCEALVNE
                     NQET"
     gene            1715..2398
                     /gene="prcA"
                     /locus_tag="VLA60_03240"
     CDS             1715..2398
                     /gene="prcA"
                     /locus_tag="VLA60_03240"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSF08400.1"
                     /translation="MALPYYVSPEQMMQDRAEYAKKGIAKGRSIIAMEYSDGVLFVAD
                     NPSASLFKISEIYDSIAFSGAGRYSEFENLRKAGIQHADVKGMMYSREDVTARSLANG
                     YSQILGTLFSQEMKPYEVELLLAQVGETPERNELYRISFDGSIVDERKFTAIGGRSET
                     ILTLLRKEIPHTCPSLKEALTLCQHAFEQGADPRSNLEGLEVAILDRTRIGRRFRRVP
                     IFEATQILA"
     gene            2683..4059
                     /gene="pafA"
                     /locus_tag="VLA60_03245"
     CDS             2683..4059
                     /gene="pafA"
                     /locus_tag="VLA60_03245"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HSF08401.1"
                     /translation="MMKRIVGLESEYGLTFSPNGRVYLPIEKILGYIFEGLIPNSWPS
                     NAFLTNGARFYQDTGCHPEYSTPECDDLLDLIIHDKAGERILESCLPIAEERLREEGL
                     SGEIFIFKNNTDSLGNTYGCHENFLMRRDVDFWKVSEQLIPFFVTRQIFSGAGKILKV
                     SGKSQYFISQRAQHIHEKTSSSTTSSRSIINTRDEPHADAEKYRRLHIILGDSNMSEF
                     STYLKVGTAMIVLSMIEDGFTIPHIELEEPVKAIRDISRDPTLKKTVKLEDGSNLTAL
                     EIQQTFWERAGEYLALQPPNKVMAEVHYEWGRVLRMLKTSPMTLVREIDWITKQWLLE
                     NYMAKKSCGWDDPRLGMMDLQYHDINRQRSLFHLLAERNHIRKMIDEDAIEQAKTVPP
                     QTTRAKVRGDFIRFARAKNRSYTVDWTYLKLNGYWEETILCMDPFCPFNRRVEELLSQ
                     MPHNRLFP"
     gene            4056..>4584
                     /locus_tag="VLA60_03250"
     CDS             4056..>4584
                     /locus_tag="VLA60_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08402.1"
                     /translation="MMSSLSPRHPSASWKILFVVCLLLASIFPIQIVPSAERNRPGAN
                     GHLSGKQGQVLWIDVPISQPTAKVSGLFLKRKIPFFPHGDTHFAGMVGIDMEDPPGMQ
                     ELSITVSSDSGSEHLSYTVQIIKEDYTVQHLTLPKNTVDLDAKTLQRVQEEKKELAEA
                     FHHVGARPLWDGPFLE"
BASE COUNT         1234 a         1209 c         1094 g         1047 t
ORIGIN      
        1 taatacccgt gatgaacccc atgcccatca agcggactac agacgccttc acgtcatcgt
       61 gggtgatgcc aatatgtccg aaatatcaac cttcttaaag gtcggcacga cggccattat
      121 catggaaatg ctggaacagc aagggcctct cccacatatc gaattgacgg atccggtcaa
      181 ggccatcaag gcggtatcac gagatctgac tgtaaaaagt tcgcttccgc taaacaatgg
      241 gcaacatacc accgccctgg ctattcagcg ggcctttctg caatcagccc atgactttta
      301 tcgaacgaga gaggtctccc ctgtcaccaa ggaggtgctt gtccgctggg aatccacctt
      361 ggataccttg gagcgtgatc cctttgagct tcggcgagaa gtcgattggg tcgctaaaca
      421 tgcattgatt caatcctata tggatcgcaa acactgttcc tggaatgatc ccagggtggc
      481 catgatggat ctacagtatc acgacgtgcg accaaacaaa gggctgtact ataccttgga
      541 acggacggga caaattgaac gtttgactct cgagctggaa gtcgaacgtg ctcgccagac
      601 tgcaccggct ttcacacggg catttttccg gggtcaatgt ttaaaacacc tgccaggcca
      661 ggtatacggc atgagttgga cttccgtcct ccttcatacc ggaaattcta ccattaagcg
      721 gattccactg atggatcctt atcgaggcac ccaacaactc acccaggaat tgtttaggga
      781 cattacctcc gtggatcagt tattatcccg actcgtgaag tcctgaatga aacaaggaac
      841 ccagtcggtc aggtaacatt gaggtcgcat gaattctgaa aacgtttccc ttccaaactt
      901 ctcagctgac ccgtgttcca gtttctacca atacctcacc tcccaacgac ccgacttact
      961 ccccatggcg cggtgggaaa actcactacc ccagccaggc ccaacagacc cttcgttgat
     1021 atccaacagg catcttccct ggccacacgg caccacggtt ctcgccttta cctatgataa
     1081 cggcgtcctc atcgccggag accgtcgcgc caccgaagga tttcaaatcg ccgcgaatcg
     1141 aatggaaaag gtctttcaaa ccgatggctt ttctgccatg gctattgcag gagctgcagg
     1201 gccctgcgtg gaaatggcaa aacttttccg catcgaactg gaacattatg aaaaactgga
     1261 cggacaaccc ttaacctgcg aaggaaaagc caatagactt gggcatatgg taaaggccaa
     1321 ttttcctatg gtcttacatg gactcgtggt cattcccctg tttgtcggct atgaccacaa
     1381 acgagaaatt ggtcgactct tcaaatatga cataacggga ggacgctacg aggatacgga
     1441 ttttcacgca gtcggctcag gggggaaaga tgcgcgatcg accctcaaag aatatttcag
     1501 aaaaaaccta ccagaggacg cggcggtcaa agtggctctt cgggccctac tcaatgccgc
     1561 agatgaagat gtcgggaccg ggggaccaga cctggtgcga cgaatcttcc cgaccgtcaa
     1621 attagttgac tcccgggggg ttcgggatgt ggatgacacc caattggcta cagtgtgcga
     1681 ggccttagtc aacgagaacc aggagacata aaccgtggcc ctgccctatt acgtttcacc
     1741 cgaacaaatg atgcaggacc gggcggaata tgccaaaaaa ggcatcgcca aaggccgatc
     1801 gatcatcgcc atggaatatt cagatggagt cctgtttgtc gccgataacc ccagcgcctc
     1861 cctgtttaaa atttctgaga tctatgacag catcgcattt tccggcgcgg ggagatacag
     1921 tgaatttgag aatctccgaa aagccggcat tcaacatgcg gacgtcaaag gcatgatgta
     1981 tagccgcgaa gatgtgaccg cacgatcact ggcaaatggc tattcacaaa ttctcggcac
     2041 gctctttagc caggaaatga aaccctacga agtggaactc ctgttggctc aggtcggtga
     2101 gacaccagag cgcaatgagt tataccgcat ctcgtttgat gggagtatcg tggatgaacg
     2161 aaaattcacg gccattggag gacgatctga aaccatctta accctcctca gaaaagaaat
     2221 ccctcatact tgcccgtccc tcaaggaagc cttaacgctc tgccaacatg cgtttgaaca
     2281 gggcgcggat cctcgatcaa atctagaagg tttggaagtc gccatcttgg accgaactcg
     2341 aatagggaga agatttcggc gagtcccgat cttcgaagct acccaaattc tcgcataacc
     2401 tctcgtacgt tcctccctcg cactcagttg atcattacct gatctgggtg tattctgagt
     2461 ataagattca ttgaaataat gcccatattt ccgagcatga tctatttcca aaacccggaa
     2521 agtgaaagat agaaatactc aagaaggcct gctcaagagg accgaaccac ttcaggagga
     2581 ttattgtcga ttggcagtca ggtccttaac acgagaagaa gtccggtggc ggagatggga
     2641 taacatgccc ttaaaaagcc tcaggagatg gaggggccgg gtatgatgaa acgcattgtt
     2701 ggccttgaga gtgaatacgg cctgacattt tcgccgaatg gacgggtcta cctccccatt
     2761 gaaaaaatcc tcgggtacat ttttgaggga ctcatcccca atagctggcc gtccaatgcc
     2821 ttcctcacga atggggcgcg gttctaccag gacacgggtt gccatccaga atactccacc
     2881 cccgaatgcg acgacctgct tgatttgatc atccacgaca aagccggtga gcggatcctg
     2941 gaaagctgtc tgcccattgc cgaagagcgc ttgcgcgaag aagggctgtc cggcgaaatc
     3001 ttcattttca aaaacaacac ggattccctt ggcaacacgt atggctgcca tgaaaatttc
     3061 ctcatgcgcc gggatgtgga tttctggaaa gtcagcgaac aactcatccc cttcttcgtc
     3121 actcgtcaaa ttttttcggg agccgggaaa atactgaaag tatccgggaa gtctcaatat
     3181 ttcatctccc aacgagcaca acatattcat gaaaaaactt cttcctctac aacgtcgtcc
     3241 cgaagcatta tcaatacacg cgatgaaccc catgctgatg ccgaaaaata ccgtcggctt
     3301 catatcattc tcggcgattc caatatgtcg gagttttcca cctacttaaa ggtagggacc
     3361 gccatgatcg tcctctccat gatcgaggac ggattcacca ttcctcatat tgagctagaa
     3421 gaacctgtta aagcgattcg agacatctct cgggatccca ctctgaaaaa aaccgtcaag
     3481 ctagaagatg ggagtaacct gaccgcattg gaaatccaac agacattctg ggaacgggcg
     3541 ggtgaatatt tggcattgca gcctcccaac aaagtcatgg cagaggtgca ttatgaatgg
     3601 ggtcgcgtgt tgaggatgct gaagacctcc cccatgacac tcgtccgcga aatcgactgg
     3661 atcaccaagc aatggcttct ggaaaattac atggccaaaa aatcttgtgg atgggatgac
     3721 ccccgattag gcatgatgga tttgcaatat catgacatta accgacaacg cagcctgttt
     3781 catctcctgg cagaacgtaa tcacattcga aaaatgatcg atgaagacgc aatagaacag
     3841 gccaaaacgg ttcctcctca aaccacacga gcaaaagtgc gaggagattt catcagattt
     3901 gcccgtgcaa aaaatcggtc gtatactgtg gattggacct atctcaaact caacgggtac
     3961 tgggaagaaa ctattttgtg catggacccg ttttgtcctt ttaatcgacg cgtagaagaa
     4021 ttgctcagcc agatgcctca taatcggctt ttcccatgat gagctcattg tccccacgcc
     4081 atccttcagc ctcatggaag atcttgtttg tcgtttgcct gctgctcgcc agcattttcc
     4141 ctattcagat cgttccgagt gcggaacgga accgaccggg tgccaatggc catctcagcg
     4201 gaaaacaggg ccaagtcctc tggattgacg tgccgatatc tcaaccgacg gccaaagtga
     4261 gtggcctatt tctcaaacgg aagattccct tcttcccaca tggtgatacc cattttgcag
     4321 gcatggtcgg aatcgatatg gaagatccac cgggaatgca ggagttgagt atcacggtca
     4381 gttccgactc cggatcagaa catctcagct acaccgtcca aatcatcaaa gaggactata
     4441 ccgtccaaca cctgacgctg ccgaagaata ccgtggacct tgacgccaaa actttacagc
     4501 gcgtccaaga agaaaaaaaa gaattggcag aggcctttca ccacgttggc gcgcgtcctt
     4561 tatgggacgg tccgtttctt gagc
//
LOCUS       DATIAL010000097         4584 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1199482, whole genome shotgun sequence.
ACCESSION   DATIAL010000097 DATIAL010000000
VERSION     DATIAL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199482"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2881)
                     /locus_tag="VLA60_03255"
     CDS             complement(<1..2881)
                     /locus_tag="VLA60_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08403.1"
                     /translation="MSLLQETGLSALLIEDNSHERIAIRRSLKKEVHTLLECESAEEA
                     IRHLERSPSEFDVIIADFKLPGMDGLTLFRTLRQMKCLSPFILLTGNGSEQIAIEAFK
                     AGVNDYLVKASESDYLNLLPALVTEVIQHHRLKIRNELLELSLKEAHERFHQFMEHYQ
                     GIFWLTDFQEPRKLLYVSPTYERIWDRPALQLAQNSLDWLEALHPKDQDRIRNAYLKL
                     NITGGYNEIYRIVKVDGSTRWIHERGLINHNEGGHPYRITRLAEDITDRTTMSEALQA
                     SEERFAQFMAHLPGLAYMKDEQYRLTYLNPEFEQVFNIDIKNWIGKTVHDVLPPDTAA
                     MLQHDDDWVLSHGQSLEMIKDIPLNGGLHQYLTCKFPILQPDRPPILGGVSIDVTGQF
                     KAEIQRDHIFSLSLDLMCSIGIDGYLKKTNPAFEKLLGYTQEELLSQPLIFFTHPDDR
                     ISTQTAFHQLAEEKDVVDFQNRIRDIRGNYLWLEWRATPQLQEKTIYAVARDITQRYH
                     ATKALRRYEQIVSTSSDFLCFLDEKNCLQAVNHGFCEYFNLPARDITGRHIRDFVGTD
                     LFESQLNSHLDDCRKGRVVCFHQQVTFPEENVRFMEVRLNPYVESDGTISGVTAIWRD
                     LTEQKRLEQHVRQTQKMESIGTLAGGIAHDFNNILMAILGWAEMGTWKHPKGDPAYKY
                     FEQIGIAGKRGRDLIQQILTFSRQNEHHPRPVDLAPIIEESLALLRATFPSNIELRFG
                     NHHNINMVFADPVQIHRIIMNLCSNAEHALRPNGGTLTIDLQAILIEKGDLQFPPTLN
                     PGRYVQIVVSDSGPGIPDHLLPKIFDPFFTTKPVGEGTGMGLSVVHGIVENLRGHISV
                     KSSNKDGTTFLIAIPEYQIPADSSLHSNPSPICPGKGHVLIVDDEVSITEVLKEFLEH
                     LGYTVTVRTHPVEALEEFRMNPHRFDCLITDQTMPTLGGDQLSQEI"
     gene            complement(2897..3358)
                     /locus_tag="VLA60_03260"
     CDS             complement(2897..3358)
                     /locus_tag="VLA60_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08404.1"
                     /translation="MMMHEPRNLTILMAEDNPHDVLATKRAWEQFGFGNHLHIVSDGE
                     ECLEYLFHQGKYLESNIFPDPDLILLDLKLPKLDGHQVLKTIRANDQFRFLPVIILTN
                     STLETDRNKGYEYGCTAFLKKPVGYANFANLLKSLRTFWSCVEPYAKPLTA"
     gene            complement(3345..>4584)
                     /locus_tag="VLA60_03265"
     CDS             complement(3345..>4584)
                     /locus_tag="VLA60_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08405.1"
                     /translation="NIHTHRAFHSLTGDLEAKYALRTNEMKEQNLAMMNLLEDMEQVK
                     NNLEDKERRLRALIDAFPSGMLIANHLGNVVFANKLIETLFGYSQQELVGQSIDILVP
                     KRFQQEHSQHRSEFLHHPSARAMGAGRDLFARRKNGSEFPVEIGLNPIVTPDGVMVLS
                     SVVDITTRKKTEAELKRVNQQLLAQNQELEAYSYAVSHDLRTPLRAIHNYADFLLEDL
                     STKVSSDQTVYLAGITTAVCEAEELVSDLLKLSRLNIKDSVPQVCHVGNIISKTLFLL
                     NFDKDVEIHQPAEWPTVLGHEHLLKQIFQNLLTNGVKFNHSSPKVLTMNWRQEPHGFI
                     TFTVEDNGIGISEQYHDKIFQVFERLHTTREYEGTGIGLAIVKKAVARLGGEIRMKSE
                     LGKGSVFSFTVPIGENHDDA"
BASE COUNT         1100 a         1039 c         1198 g         1247 t
ORIGIN      
        1 ggatttcttg agataactgg tctcctccca gtgtgggcat cgtttgatcg gtgatgagac
       61 agtcgaatcg atggggattc atgcgaaact cttcaagggc ctccaccgga tgagtccgga
      121 ccgtgacggt atagcccaga tgctccagaa attctttcaa gacttcggta atcgatacct
      181 catcatccac aatcagcaca tgtccctttc cgggacagat gggggaaggg tttgaatgaa
      241 gggaagagtc agcgggtatc tggtactcag ggatagcgat cagaaaggtg gttccgtcct
      301 tattagaaga tttgaccgaa atgtgtcctc ggagattttc tacaatgcca tgtaccacgg
      361 acaatcccat tccggttcct tctccgacgg gtttggtggt gaaaaacggg tcgaaaattt
      421 ttggaagaag atgatcggga attccaggac cggaatcact gacgacgatc tgcacatacc
      481 ttccgggatt cagggtcggg gggaattgga ggtcgccttt ttcaatgagg atggcctgaa
      541 ggtcgatggt cagggttccg ccattggggc gaagggcatg ttccgcattc gaacagaggt
      601 tcatgatgat gcgatgaatt tgaacggggt cggcaaacac catatttatg ttgtgatgat
      661 taccaaaacg cagctcaatg tttgagggaa aggtggcccg gagaagagcc agcgattctt
      721 cgatgatggg ggccagatcg accgggcgag gatggtgttc gttttgccgg ctgaatgtga
      781 ggatttgctg gatgagatcc cgcccccgtt tccccgcgat tccgatttgc tcaaagtact
      841 tgtatgcggg atcgcctttg ggatgtttcc atgtccccat ttctgcccat ccgagaatcg
      901 ccatcaggat attattgaaa tcgtgagcga ttcctcctgc cagggttccg atggattcca
      961 ttttttgtgt ctgtctcaca tgctgttcca gacgtttttg ctcggtaagg tctctccata
     1021 tggcggtcac gcctgaaatc gttccgtccg actcgacata gggattcagt cttacctcca
     1081 tgaagcgcac gttttcttct ggaaaggtta cctgctgatg aaagcagacg acccgccctt
     1141 ttcgacagtc atctaaatga ctgttgagct gagattcaaa tagatctgtt cccacaaaat
     1201 cccgaatgtg ccggccggtg atgtcccggg caggaagatt gaagtattca caaaatccat
     1261 ggttcacggc ttgcaggcaa ttcttctcat ctaaaaagca gagaaagtcg gagctggttg
     1321 acacaatttg ttcataccga cggagtgctt tagttgcgtg ataccgctgg gtaatatccc
     1381 gtgccacggc atagatggtt ttttcctgaa gctggggagt ggcgcgccat tccagccaga
     1441 ggtaattgcc acggatatca cggatgcggt tctggaaatc taccacatcc ttttcttcag
     1501 caagttgatg gaacgctgtc tgggtggata tccggtcatc cggatgagtg aaaaagatca
     1561 atggttggga taacagttct tcctgggtgt atccaagaag tttttcgaag gcaggattgg
     1621 tttttttgag atatccatcg ataccaatgc tacacatgag atccagggag agggaaaaaa
     1681 tgtgatcccg ttggatttcc gccttgaatt ggccggtgac atcgatcgaa acacctccca
     1741 gtattggtgg gcggtcaggt tgcagaatag gaaatttgca cgttaaatac tgatgcaggc
     1801 cgccattgag tggaatgtcc ttgatcattt ccagggattg accatgggag agcacccagt
     1861 catcatcgtg ttggagcatg gcggccgtgt cgggaggcaa gacgtcgtga acggttttgc
     1921 ctatccagtt tttgatatca atattgaaca cctgttcaaa ttccggattc agatatgtca
     1981 aacgatactg ttcgtctttc atataggcca aacccggaag atgggccatg aactgtgcaa
     2041 agcgttcctc gctggcctga agggcttctg acatcgtggt gcggtcggta atatcttcag
     2101 ccagccgtgt gatccgatac ggatgccccc cttcattgtg attgatgagt ccgcgttcat
     2161 ggatccagcg agtcgacccg tctactttca cgattcgata aatctcgtta tatcctcccg
     2221 tgatattcag cttcagataa gcgttcctga tacggtcctg atctttggga tgaagggcct
     2281 caagccaatc caacgagttt tgagcgagct gaagagccgg gcggtcccaa atcctttcat
     2341 aggtcgggct gacatacaat aattttcttg gttcttgaaa gtcggtcagc caaaaaatcc
     2401 cctgataatg ctccatgaat tggtgaaacc gttcatgggc ttctttgagt gacaattcca
     2461 gcaactcgtt tcggattttc aaacgatgat gttggatgac ttcggtcact agcgccggca
     2521 ataaattcaa atagtcgctt tcggacgctt tgaccaaata gtcattcact ccggctttga
     2581 aggcttcaat ggcaatctgt tcggatccat tgccggttaa gagaataaac ggagataggc
     2641 atttcatctg acgcagggtg cgaaatagcg tgaggccatc catgcctggg agtttgaagt
     2701 cggcgatgat gacgtcaaat tccgaaggag agcgttcaag atggcgaatg gcttcctcag
     2761 cactttcaca ttcaagaagg gtgtgaacct ccttcttgag ggaacggcgg atcgcaattc
     2821 gctcatggga attgtcttcg atgaggagag ctgataatcc ggtttcctgg agaagggaca
     2881 tgggccaccc gttcgtttag gctgttaatg gcttggcata aggttccaca caactccaga
     2941 aggttcgtag ggattttaag agattggcaa agttggcgta cccgaccggt tttttgagga
     3001 acgccgtaca tccgtattca tatcccttgt ttcggtcggt ctcaagggtt gaattcgtga
     3061 gaatgatgac aggtaaaaac cggaactgat cgttcgcccg aatcgttttg agaacctggt
     3121 gtccatccag ctttggaagt ttgaggtcca gaagtatgag gtcagggtca ggaaagatgt
     3181 tcgattcgag atatttccct tgatgaaaca ggtattccag gcattcttcg ccatccgaca
     3241 cgatgtgcag atgattccca aagccgaatt gttcccaggc acgtttggtg gccaggacat
     3301 catggggatt atcttccgcc attaaaatcg ttaaattccg tggttcatgc atcatcatga
     3361 ttttctccaa tcggtaccgt aaatgaaaag acgctgcctt tgcccagttc ggacttcatg
     3421 cgtatctctc cgccaagcct ggctacagcc ttcttgacaa tagccaaacc gatgcccgtt
     3481 ccctcatatt cccgggttgt gtggagccgt tcaaagacct ggaatatttt gtcatgatat
     3541 tgttcggaaa ttccaatgcc gttatcctcc accgtgaagg tgataaagcc atgaggttcc
     3601 tgcctccaat tcatggtcaa gacttttggg gatgagtggt tgaatttgac tccattggtc
     3661 agaaggttct ggaaaatctg tttcagcaga tgttcatggc caaggactgt tggccattca
     3721 gctggttgat gaatttccac gtctttatcg aaattcagca ggaaaagcgt tttggaaatg
     3781 atgttgccga cgtgacagac ctgaggaacg gaatctttga tatttaaccg tgagagttta
     3841 agaagatcag agaccagctc ttccgcttcg catactgcgg tcgtaatgcc ggccaagtac
     3901 acggtttgat cggatgaaac ctttgtggac aagtcttcta acagaaaatc cgcataatta
     3961 tgaatggctc tcaagggtgt tcgcaaatcg tgtgagacgg cataactata ggcttccaac
     4021 tcctggtttt gtgccagaag ttgttgatta acccgtttca gctcggcctc ggtttttttt
     4081 ctggtcgtga tatcgacgac agagctaagg accatgacgc cgtcgggtgt cacaatagga
     4141 ttcagaccga tttctaccgg aaattcactc ccgttcttgc gacgggcaaa cagatcccgt
     4201 cccgctccca tggcccgggc actcgggtga tggaggaact cggaccgatg ttgggaatgt
     4261 tcctgttgaa atcttttggg gacgaggata tcaatggatt gccccactaa ttcctgttgt
     4321 gaatagccaa agagcgtttc gattaatttg ttggcgaaga ccacgttgcc gaggtgattc
     4381 gcaatcaaca tgccgctggg gaaggcatca ataagtgccc ggagccgacg ctccttgtcc
     4441 tccaggttat ttttaacctg ttccatatcc tccaaaagat tcatcatcgc caaattctgt
     4501 tccttcattt cattcgttcg cagggcatat ttggcctcaa ggtctcctgt taaggaatga
     4561 aatgcacgat gggtgtggat attc
//
LOCUS       DATIAL010000098         7548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1092054, whole genome shotgun sequence.
ACCESSION   DATIAL010000098 DATIAL010000000
VERSION     DATIAL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092054"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1430..2845)
                     /locus_tag="VLA60_03270"
     CDS             complement(1430..2845)
                     /locus_tag="VLA60_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HSF08406.1"
                     /translation="MLPSLPLPTRLASWNPTATTRRFFPHGQLSAGQLVTLGGIALIT
                     IGTFLLKTPWATPPGKSLSFLNALFTATSAVTVTGLIVADTEKDFTVFGQIVILTLIQ
                     LGGLGYAMLATVLLIAMGQRIGLRNRMMVAEALSSLDLAGSIRFVKTILIITFTFEGV
                     GASILAARFSQEMPFLEACWHGIFHAISAFNNAGFSTFSQNLIPYRNDVLVNVSISLL
                     FVLGGIGFIVFRDLLSYYRKERYRIQTHTRLALLVSVSLWVIGTIGFYLLEMHSPATL
                     GSQTPAEGAMVAAFHSGAARTAGFNALDLSLLRPSTLYMLILLMIIGGSPGGTAGGIK
                     TTTFGIVCLSVWAVFRKRLDVEFHYRRMPPHLVLQALAIMFLAIGAVTTLTFFLAFIE
                     NKPFLALMFEVASALGTVGFSVGNGGVLSLSAILTDFGKVIILCSMFLGRFGPLLVGL
                     VSLTPPVRMLYRFPQAKVAIG"
     gene            complement(3070..3492)
                     /locus_tag="VLA60_03275"
     CDS             complement(3070..3492)
                     /locus_tag="VLA60_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08407.1"
                     /translation="MEAPNSSVSPQILPKNQTIILVEDQSDLRWLLKRRLEMNGYTCL
                     EAANGREALSLIQQHPTIRLILSDYLMPEMNGLQLLQALRQNSMNRKIPFILITASWS
                     DQLQHQGMNAGAFAILAKPYHHGELVQLLEQGISTQAA"
     gene            complement(3982..5970)
                     /locus_tag="VLA60_03280"
     CDS             complement(3982..5970)
                     /locus_tag="VLA60_03280"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HSF08408.1"
                     /translation="MSTIPKTLQTLIIEHVEPELDGGRYPVKRIVGENLDVTADVFKE
                     GHDTIGAVLRYKLLGQTEWSETPMHHVDNDRWAGSFLLSENTRYLYSVGAYVRSFETW
                     RIELTKKHGVLPDLSSELLEGEAQVKEAMTRAKGPDKSELKLWLEKWKSSSDQEGRIA
                     IALDPIVATLVDRHEQRAAWSTYEKELEVIVDRERARYGAWYEIFPRSEGTVPGKGGT
                     FKDCELRLPAIRDMGFDVLYLTPIHPIGETNRKGRNNSLKAKPGDPGSPWAIGSRHGG
                     HDAVEPALGTMNDFDHFQQAVRKHGMEVAIDFAINATPDHPYVTQHPDWFKQRPDGTI
                     KYAENPPKKYEDIYGFDFYTEAWQDIWQEMKRVLLFWIEHGVKIFRVDNPHTKPVIFW
                     EWLIREIQLEHPDVLFLAEAFTRPKMMRVLAKAGYTQSYTYFTWRNSKGEMTEYLTEL
                     TRTQMQEYFRPNFFANTPDILPEILQQGGRPAFKFRLVLAATLSPTYGIYNSYELCEN
                     KAIPGTEEYQDSEKYEIRHWDWDRPGNIRDYITRINQIRRDHPALHTFTNLQFYQSDN
                     DHILFYGKMNADKTDILLFAINMDPYTIHEARLRIPIEACGIGEQDTYQLHELIQDYR
                     HQVVGGDYTIRLDPNDEPAAIFALRRRTRRESDFDYFM"
     gene            complement(5954..6112)
                     /locus_tag="VLA60_03285"
     CDS             complement(5954..6112)
                     /locus_tag="VLA60_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08409.1"
                     /translation="MFRKPSLEPEGLLTYRDRPDKSGLLDGEQLPIVIYRNIQGATHE
                     GVRHVYYS"
     gene            complement(6112..>7548)
                     /locus_tag="VLA60_03290"
     CDS             complement(6112..>7548)
                     /locus_tag="VLA60_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HSF08410.1"
                     /translation="EYTEGEPEMYLLPLQYLPADRMASLVDSPAAIARVRVKMKDGEQ
                     EGLLIDAMWDREFQKMLLDSISRNRRFNGPAGDLATQATKIFRRQLQKEVPTLEPTLL
                     KGEQSNSSVLFGHDFILKLYRRAEVGVNPDFEIGRFLTNKGFPHIAPLAGSIEYQRDN
                     GDLLTFGILQKFIQNEGDAWKYTLDELSRYLEEALTHPTAITTSSIPQKSLMALVDEE
                     IPTGAREWIGPYLEEARLLGLRTGELHAALASDFEDPEFKPEPFTDFYRRGLYQSMLG
                     TVNMNFPLLRTQVKGLQDPVQSIAKHVLEGEGRLRKRLLTIRDRKLTCTRIRCHGDYH
                     LGQVLYTGKDFIIIDFEGEPARPLNVRRLKESPLRDVAGMLRSFHYAAHASSIGLVEG
                     VRPEDFSLLEPWARYWQIWVSVSYLKAYLSVKEVRDILPPSSDDIQILLNGYLLQKAI
                     YELGYELNNRPNWVRIPLDGILQILEVD"
BASE COUNT         1838 a         1795 c         2023 g         1892 t
ORIGIN      
        1 cgtacgtttt ccttgcaggc ttcctttggt ggccccgcct cgatggattc gttaggggca
       61 aggccatcca gtaaggtgag agattatttt gagttttttt cttcagtgtt ttctttaccg
      121 ggcgctaccg ggtacctcga cgtttaagta cttccaggac ctctctaaaa aatcctgaca
      181 ataggcgtgc tccttgcggt ccttccatga caaggggatt gcgtcaggcg acccccgaca
      241 actcagccgt tttaatcctt ccactcgtaa ggataccctg ctcatgttga aagccctcgt
      301 ccttcgaacc ttgtcctatt taaccgatga tcaaagtgaa gatcaaattc gtacccggga
      361 gtgccgtatt gttctgtctg tcatctttgg aaaatgttca tcttccattc caggagtcgt
      421 tggcccaatg cgtaccggcc ggtcgaagtc agaggacccc tgagtccggc tctaagggcc
      481 aatgtggaaa gggccttcgc ctctctgaaa agtcaaatcc tgtttggatc caccaatttc
      541 cctaggagga ttcacaagag acgaacgaat tcagtcgagc tgggatcttt taccaattcc
      601 actaattatg cactatcttt cggccatcct ctggaaagcg acgaaatgaa aggagaggtg
      661 gctggcttta cacaggcaac acgtctttga ccctcctgca tggcagaacc gtcatggaat
      721 aattaagtta atcaaatcaa aagaagacgc cgtgttttct tcatatttca tgcaatgaga
      781 aaactatcaa acccttcaca gcgtaaggcc atttgataga aaaaatccca ccgggaagcc
      841 tcatgagact tcatgggatg cgcgaaaatg cactttaaga acggagcgaa attgctcaag
      901 ggcagcattt caatcttggg tgatgcaaac aggactgaat tgaaccggtt acttgagtcc
      961 tgatcgtgga aaagaacaat gctttgagtg gtgcagggaa ggctcgcttc tttttaatgt
     1021 tgattccgac ttggcgggca tgcctacaga ggggattggg cgagagaggt tagatgggtg
     1081 ataaaaaact agttgaacca gaaaataaat atggcctcag tcaacatgcc attaccgacg
     1141 attgccggga attattgtgg gccctgagag accattactg tggcgagtga ggctatagaa
     1201 gtattttgtc ctaaacggat cggtctttca ccgtgcaggg aagttctcga agagcaccca
     1261 tgtccatgag aactgcccca tcaagcatcg gcgctcttaa gatgaaccgg agaggaaaag
     1321 tgtgatgcct atcacacgga accaggctga atgccctgat gtagggtggt ggaagatccc
     1381 tcatcacatc tttccggtga atggggcagg tgttgtcatg tgtcccatct taccctatcg
     1441 caactttggc ttgaggaaaa cgatagagca tgcggacggg tggagtcagg gagacgagac
     1501 caaccaggag aggtccgaat ctcccgagga acatactgca taaaataatg acctttccaa
     1561 aatccgttaa gatcgccgaa aggctgagca cgcctccgtt tccgacggaa aacccaactg
     1621 ttcccaatgc ggaggccact tcaaacatga gagccaaaaa aggcttattt tcaataaagg
     1681 cgagaaaaaa ggttaacgtg gtgacggctc caatggccag gaacatgatt gccagagctt
     1741 gcaagacgag atggggaggc atccgccggt agtgaaactc cacatccagt cgttttcgga
     1801 agacggccca gactgataga cacacaatgc cgaacgtggt ggttttaatt cctccagcag
     1861 ttcctcctgg tgatcccccg atgatcatca gcaaaattaa catatacaac gtgctgggtc
     1921 ggaggaggct caggtccagc gcattgaatc ctgccgtccg ggcggctccc gagtgaaagg
     1981 cggcgaccat ggctccctct gcgggagttt gcgagccaag cgtggccgga ctatgcattt
     2041 ccaacagata aaatcctatc gttccaatca cccataagct taccgaaacg agtaaggcca
     2101 gcctcgtatg ggtttgaatt cgatagcgtt cttttcggta ataactcagc aggtcgcgga
     2161 agacaataaa cccgatgccg cccagaacga aaaggaggct aatggaaaca ttgaccagga
     2221 catcgtttcg ataggggatc agattttgcg aaaacgtcga aaatcctgca ttgttaaagg
     2281 cggaaatggc gtggaaaatg ccatgccagc aggcttccaa aaagggcatt tcctgggaaa
     2341 accgcgcggc gagtatggac gcgcccacgc cttcaaaagt aaaggtgatg atcaaaatag
     2401 ttttgacaaa acgaatagat cccgccaaat ccaatgaact gagcgcctcc gccaccatca
     2461 tgcggtttcg taatccgatg cgttgtccca tggcgatgag aagaacggtc gccagcatgg
     2521 cataccctaa tccgcccaat tggataagtg tcaaaatgac gatttgtccg aatacggtga
     2581 aatctttttc cgtgtccgcc acaatcaggc cggtcaccgt tacggccgaa gttgccgtaa
     2641 agagggcgtt gagaaaactt aatgatttac cagggggggt ggcccatggg gtcttcagga
     2701 ggaacgttcc gatagtgatc aaggcaatgc cgcctagtgt gacgagttgg ccggctgaca
     2761 gttggccgtg ggggaaaaat cgacgagtcg tggcggtcgg attccacgag gccagacgag
     2821 taggaagtgg taatgaaggc agcatgaagg ctaaggaacg aattctttta ccttctcaca
     2881 taagcagttc cgaaaaataa tcgaaattca atgtcgctca gctgagtcaa tcgggtgaga
     2941 agtctcattc acaaattttc ttctaggcaa tttacattcc ggggggaaaa caaaggaagc
     3001 agagaaattg tctggcactt ggactggcaa tcttgtctga ggagatgttt tctgtcaaga
     3061 ccgccggcat caggcggctt gagtggatat tccttgttca agaagttgga ccagttcccc
     3121 gtgatgataa ggcttggcga ggattgcaaa ggcccctgca ttcatgcctt ggtgctggag
     3181 ctgatctgac caactcgccg tgatgagtat aaacgggatt ttccgattca tggagttctg
     3241 ccgaagggct tgaagcagtt gcaggccatt catttccggc atcagatagt cggacaggat
     3301 cagacgaatg gttgggtgct gctgaataag agacaaggct tctctaccgt ttgccgcttc
     3361 caggcaggta tacccattca tttccagcct acgtttcagt agccatcgga ggtcggattg
     3421 atcctccacc aggattatgg tttggttctt gggcaggatc tgaggggaga ccgatgagtt
     3481 cggtgcctcc aaatccctcc aaggtgagat tgtcatgtgc atgacagcag agaagtgcaa
     3541 ggaacattcc atgaatatta ctaggagtcg gcttcggggg ggctaagaga cattcctaat
     3601 tactgggtat ttaaaggtat attcagaaat gtcaaaaacg gggctgagaa gcgaatgtga
     3661 ttggcttcaa aaggaaagaa acgttgtctc aaaaggagac aaactgtctc ttctgttgct
     3721 gggatttttc tcctgagatg gcctaagcgc gaggggttgg gtttttctgt ttctcattat
     3781 ccttgcagat gagtcgcggg gatcgatctg aagcgcggca aataggggtt gtgtgcctat
     3841 attcgtcggt tatgtgggag agaggaggtg ctcgttgaac attgcgccta tgaaggctct
     3901 gtcgaagttc aggcccctgc caagaattgg caggggctgt tttttcattg tggggtaaga
     3961 aaacctgcgg atcgttctct gttacatgaa atagtcaaag tcgctttcac gacgggtccg
     4021 gcgtcgaagt gcgaaaatcg cggccggttc gtcgttaggg tccaatcgaa tggtgtagtc
     4081 acctcccacg acttgatggc ggtaatcttg aatgagttca tgcagttgat aggtgtcctg
     4141 ttcaccaatg ccgcatgctt caatcggaat tcgcaggcga gcctcatgga tggtataggg
     4201 atccatattg atcgcgaaca gaagaatgtc cgtcttgtcg gcattcattt ttccatagaa
     4261 aagaatgtgg tcgttgtcgg attggtaaaa ctgcagattg gtgaaggtgt gtagggcagg
     4321 gtgatcccgt cggatctggt tgatgcgggt gatatagtca cggatgtttc ccggacgatc
     4381 ccaatcccag tgccggattt catatttttc cgaatcctga tattcctcgg ttccgggaat
     4441 cgccttattt tcgcacagtt catagctatt gtagatccca taggtcgggg agagggtggc
     4501 ggccagcacg agccggaatt taaaggccgg ccgacctccc tgttggagaa tttccggcag
     4561 gatgtcgggt gtattggcga aaaagttcgg ccggaaatac tcttgcattt gggtccgggt
     4621 taattcggtc aggtattcgg tcatttctcc tttggaattt cgccacgtaa aatacgtgta
     4681 ggactgggta taaccggctt tcgccaggac ccgcatcatt ttaggtcggg tgaaggcttc
     4741 tgccagaaac aggacatccg gatgctccaa ttggatttcc cgaatgagcc attcccaaaa
     4801 gatcacgggt ttggtgtggg gattatccac tcgaaaaatt ttgactccat gctcaatcca
     4861 aaagaggagg acccgcttca tttcctgcca gatgtcctgc caggcttcgg tatagaaatc
     4921 aaatccataa atgtcctcat attttttggg agggttctcg gcgtatttga tcgtcccgtc
     4981 agggcgttgc ttgaaccagt caggatgttg tgtgacgtag ggatgatccg gggtggcatt
     5041 gatggcgaaa tctatggcga cttccattcc gtgttttctg acggcttgtt gaaaatggtc
     5101 aaaatcgttc atcgtgccga gagccggttc gaccgcatca tgtcctccat gtcgacttcc
     5161 gatggcccag ggactgcccg gatcgcccgg cttggccttc aaactgttgt tgcggccttt
     5221 gcgattggtc tctccgatgg gatgaatagg ggtgagatag aggacatcga atcccatatc
     5281 ccggatggcc ggaaggcgta gctcacaatc tttaaatgtc cccccttttc ccggcacggt
     5341 tccttctgag cggggaaaga tttcatacca ggcgccatac cgggcacgct cacggtcgac
     5401 aatcacttcc aattccttct cataggtgga ccaggcagcc cgctgttcgt gtcggtcgac
     5461 gagtgtggcc acaattggat ccagggcgat ggcgatccgg ccttcctggt cggaagacga
     5521 cttccatttt tccagccaga gtttcagctc ggacttatcg ggacctttcg ccctggtcat
     5581 cgcctctttg acttgcgctt ccccctccaa taattcactg gaaaggtccg gaagcacccc
     5641 atgtttcttg gtcagttcaa ttctccaggt ttcaaaactc ctgacatagg ctcccacgga
     5701 atacagatat cgggtatttt ctgaaagaag aaatgatcca gcccaccggt cattatcgac
     5761 atgatgcatg ggggtttccg accattccgt ttgccccagc aatttgtaac gaaggacggc
     5821 cccgatggtg tcgtgtcctt ccttgaagac gtcggctgtg acgtccaggt tttccccgac
     5881 gatccgtttg accggatagc gtcccccatc cagttcaggc tcgacatgtt caatgatcag
     5941 ggtttgtaac gttttaggaa tagtagacat gacgcactcc ttcgtgtgtg gctccttgga
     6001 tattcctgta gatgacgatt ggcaattgtt ccccgtcaag gaggcctgat ttgtctggac
     6061 gatcgcgata agtcagtagg ccttccggtt caaggctcgg tttgcgaaac atcagtccac
     6121 ttctaaaatt tggagaatcc catccagggg gatacgcacc caatttggcc ggttattgag
     6181 ttcatagccg agttcataaa tcgctttttg cagtaaatat ccattcagca aaatttggat
     6241 gtcgtccgat gacgggggca gaatgtcacg gacttctttg acggacaggt atgccttgag
     6301 gtagctgacg ctgacccata tttgccaata gcgggcccaa ggttcgagta aggaaaagtc
     6361 ttccggtcga acgccctcga ccaatccaat agaggaggca tgcgccgcgt aatgaaacga
     6421 tcgcaacatg cccgcgacgt cgcgcagggg cgattccttg agtcgtctga cattcaacgg
     6481 cctggccggt tcgccttcaa aatcgatgat gatgaaatct tttccggtat agagcacctg
     6541 gcccagatgg tagtcgccat ggcaccggat gcgggtgcag gtgagtttcc ggtcccgtat
     6601 ggttaacaga cgtttgcgta atcggccttc tccttccaac acgtgtttcg caatggattg
     6661 cacagggtcc tgaagccctt tgacctgagt gcgaagaaga ggaaagttca tgttgaccgt
     6721 tcccagcatg ctctggtaca gaccccgccg gtaaaaatcg gtaaagggtt ctggtttaaa
     6781 ctccggatct tcgaaatcgg aggcgagcgc ggcgtgcagt tctcctgttc gaaggcccaa
     6841 caaccgggct tcttccagat acgggccaat ccattcacgg gcgccggtcg gtatctcctc
     6901 gtcgaccaga gccatcagcg atttctgagg aattgatgag gtggtaatgg ctgtgggatg
     6961 cgtgagcgct tcttccagat atcgactcag ttcatccaag gtatatttcc aggcgtctcc
     7021 ttcgttttga atgaatttct gtaagatacc aaatgtcagg aggtcgccat tgtcccgttg
     7081 atattcgatg gatcctgcca gaggggcgat gtgcggaaac cctttattgg ttaaaaaccg
     7141 tccgatttca aagtcaggat tcacgccgac ttcggcccgg cggtagagtt ttaaaatgaa
     7201 atcatgtcca aataagacag aggagttgct ttgttcaccc ttgagcaatg ttggttcaag
     7261 ggtcgggacc tctttctgaa gctgccgtcg aaagattttc gtggcttggg tggccaaatc
     7321 gcccgccgga ccattaaacc ggcggtttcg ggaaatacta tccaacagca ttttttgaaa
     7381 ctcccgatcc cacatagcat cgataagaag gccttcctgc tcaccgtctt tcattttgac
     7441 tcgcacgcgt gcaatggctg ccggagaatc caccagggag gccatacgat cggccgggag
     7501 atactgcaag ggtaagaggt acatttcggg ttctccctcg gtatattc
//
LOCUS       DATIAL010000099         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1199932, whole genome shotgun sequence.
ACCESSION   DATIAL010000099 DATIAL010000000
VERSION     DATIAL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199932"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1380
                     /gene="glyA"
                     /locus_tag="VLA60_03295"
     CDS             118..1380
                     /gene="glyA"
                     /locus_tag="VLA60_03295"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HSF08411.1"
                     /translation="MRHSVGTLKAIRDTDYEVYAAIRAEEKRQRENLVLIASENYASP
                     AVLGAQGCLMTNKYAEGYSGRRYYGGCQHVDTVEDLAIARAKEIFGCEHVNVQPHSGS
                     QANMAAYLSVLKTGDTILGMDLAHGGHLTHGSRVSFSGKLFQAFSYGVDRETEEINYD
                     VVEQQAQEVRPRMIVVGASAYSKIIDFPRFQAIAKSVGAYLLVDIAHIAGLIAVGLHP
                     SPVPYADFVTTTTHKTLRGPRSGMIMCKEEHAKAVDKMVFPGTQGGPLMHVIAAKAVA
                     FKEALSPGFKTYQQQVLANAKELAEGFLKRGYRVVSGGTENHLFLLNLTSKGVTGKEA
                     EAALNASGIVVNKNAVPFDEKPPAVASGIRIGTPTVSTRGMQKPEMEQILSWMDEVIQ
                     HSQEASLHKRILRDVKDLCSRFPIFHPY"
     gene            1426..1890
                     /gene="nrdR"
                     /locus_tag="VLA60_03300"
     CDS             1426..1890
                     /gene="nrdR"
                     /locus_tag="VLA60_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HSF08412.1"
                     /translation="MKCPFCDQLEDKVIDSRTAREGEVIRRRRECLGCKRRFTTYERI
                     EENLPMVVKKDNRREPFDRAKILAGLKKACEKRPVSIGALGAAVDRIEKRIQDLGETE
                     VPSRTVGEEVMRALKELDPVAYVRFASVYREFKDIDQFMDTIRALTQDTRMS"
     gene            2017..3153
                     /locus_tag="VLA60_03305"
     CDS             2017..3153
                     /locus_tag="VLA60_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF08413.1"
                     /translation="MPSPKPSPRSPRITKRSPRRSKAKVTSSRVAIIGGGHGGTALME
                     IFAEDPLVTVVGLSEIRPHTMGVRLAKKIGIPVLRRYQDLLKIKDVDLVIDVTGDPDV
                     EKTLLESHAPHLALVGGASAKFMWQLIEARIRASAEIENTLTRYQSLFRLYVKEEAEG
                     AVDEERTRIACEIHDGLVQTLVGVSLKMERVRELVAEDPPKCLTMLNQTTVQLKHAIQ
                     EAREVVYNLRPGQYDHLAFIPALSNYLKAYEREHRIRTEFEGSGDESQLDPKAKVFVF
                     RMVQEALSNVAKHAGATKVIVKVALKKDVLKATVSDNGQGFDVKAEGQNPEKWDHFGV
                     RSMTERARMLGGNVQWVSKPGTGTKVEIFIPLVLKEKVPYAQND"
     gene            3137..3814
                     /locus_tag="VLA60_03310"
     CDS             3137..3814
                     /locus_tag="VLA60_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF08414.1"
                     /translation="MPKTTKVLIVDDHRVVREGLCAILETKDDIQVVGEAKDGGEAVE
                     QTRLLMPDVILMDVSMPGMTGVEATRIIKREFPHIGVVALTMYEEQQYIFDLVRAGAT
                     GYLLKDSDSAQIVAAIRTIARGESLIHPSVASKILVEFSLLSEGKGKKRGILEHDLTD
                     REITVLQLVADGKTNKEIANVLDLSEKTVKNHVRNIFHKLHVFDRTQAAILAIRKGII
                     ELEPRKM"
BASE COUNT         1010 a          905 c         1098 g          953 t
ORIGIN      
        1 catcgggatg tggttcctaa gattaatgtg acccttgtga agaaagctac agaagaggct
       61 actcatgaag ggggatcaga caaagatcag gccgatgaag cggttgtcga gtaagcgatg
      121 agacattccg tcggtacact caaagcgatc cgtgacactg attatgaagt gtatgcggcc
      181 atccgggctg aagaaaaaag acagcgggaa aacttagttc ttatcgcgtc agaaaattac
      241 gccagtcccg cggtcctggg tgcccaaggc tgtttgatga ccaacaaata cgcggagggg
      301 tattccggcc ggcgatatta cggcggatgc caacatgtgg atacggtcga ggatttagcc
      361 attgcgagag ccaaagaaat tttcgggtgt gagcatgtga atgttcagcc ccactcgggt
      421 tctcaagcca atatggcggc atatctttct gtcctaaaaa caggagatac catcctaggg
      481 atggatctgg cccatggtgg gcatcttacc cacggcagcc gtgtaagttt ttcaggaaaa
      541 ctgtttcagg cgttttccta cggagtggat cgggaaactg aggagatcaa ttatgacgtg
      601 gtagagcagc aggctcaaga agtccgccct cgtatgattg ttgtgggtgc cagcgcgtat
      661 tccaaaatta ttgacttccc tcgttttcag gccatagcga aatcggttgg tgcctatctt
      721 cttgttgata ttgcccacat tgcgggtttg atcgcagtcg gattgcatcc aagcccggtt
      781 ccctatgctg actttgtcac caccacaacc cataaaacgt tgcgtggccc acgttccggc
      841 atgattatgt gcaaagaaga acatgccaaa gccgttgaca aaatggtgtt ccctggaacg
      901 cagggtgggc cgcttatgca tgtcattgca gccaaagcgg tggcttttaa agaagccttg
      961 tctcctggat ttaaaaccta tcaacaacaa gtgctggcga acgccaagga gttagctgag
     1021 ggctttttga agcggggata tcgggtggtg tctggtggga ccgagaatca tttgttcctc
     1081 ttgaatctga catccaaagg cgtgacagga aaagaagccg aagccgccct aaatgcctcc
     1141 ggcattgtgg tcaataaaaa tgcggtaccg tttgatgaaa aacccccggc tgtcgcaagc
     1201 ggcattcgta ttggcacgcc gacggtttca actcgtggca tgcagaaacc cgagatggag
     1261 cagattctct cctggatgga tgaggtcatc caacattctc aggaagcgtc cctacataaa
     1321 cggattctgc gggatgtcaa agacctgtgc tcacgatttc cgatctttca tccatactaa
     1381 ccttcctcca ccgccttcca tccttaaggt aagggccgct ctttcgtgaa atgtcctttt
     1441 tgcgaccaat tagaagataa ggtcattgat tcgcggaccg cacgtgaagg agaagtgatc
     1501 aggcgccgtc gcgaatgttt aggctgtaag cggcggttca ccacctacga acgaatcgaa
     1561 gaaaatttgc cgatggtggt caaaaaagac aatcggcgag agccgtttga tcgggccaaa
     1621 attttagcag gcctcaaaaa ggcctgcgaa aaacggccgg tcagtattgg agcgctaggg
     1681 gccgcggttg atcggattga aaaacgtatt caggaccttg gagaaacaga agttccaagc
     1741 cgaaccgtcg gagaggaagt catgcgggcc ttgaaagagt tggatcccgt cgcgtatgtt
     1801 cgatttgcct cggtgtatcg tgaatttaaa gacatcgatc aatttatgga taccatacgg
     1861 gccttaaccc aagacaccag gatgtcctga ttcatgctta tttgtctgtg gcggttagcg
     1921 agtctctctg ttttgtccgc gtcataacgt gacaagggta gagatcttct cctcgaaaag
     1981 atcttccacc tattccgatc atgaaagtgg gtgtctatgc catcccccaa accctccccc
     2041 cgttccccac gcatcaccaa acgtagtccc cgccgatcaa aggcaaaagt gacctccagc
     2101 cgggtggcga ttattggtgg agggcatggt gggaccgcac ttatggaaat ttttgcggaa
     2161 gatcccttgg tgaccgtggt agggttgagt gaaatccgtc cgcacaccat gggcgtccgt
     2221 ctcgctaaaa aaatcgggat tcctgtcctt cgccgttatc aagatttgct caagatcaaa
     2281 gatgtggatc tggtgatcga cgtgacgggc gatcccgatg tggagaagac cctgcttgag
     2341 tcgcatgccc ctcacttggc attggtgggg ggcgcgagcg ccaagtttat gtggcaattg
     2401 attgaagccc gaatccgggc ttctgccgaa attgaaaata ccctcacccg gtatcaatcg
     2461 ctgtttcgtt tgtacgtcaa agaggaagcc gaaggggcgg tggatgagga gcggacgcgc
     2521 attgcctgtg agatccatga tggtctggtg caaactctgg tgggagtgag tttgaagatg
     2581 gagcgtgtcc gtgaattggt ggcagaggat ccccccaagt gtttgacgat gctcaatcag
     2641 acgaccgtgc aattgaagca tgccattcag gaggccaggg aggtggtcta taaccttcgc
     2701 ccggggcaat atgaccattt agcctttatt cccgcgttgt caaattatct gaaggcctat
     2761 gaaagggagc accggatacg gactgagttt gaagggagtg gggatgaatc ccaacttgat
     2821 cctaaagcga aagtctttgt ttttcgcatg gtccaggaag cattaagcaa tgtggccaaa
     2881 catgccggag ctaccaaggt aattgtaaaa gtagcgctca agaaagatgt cttgaaagcg
     2941 accgtttctg ataatggcca gggctttgac gtcaaggcag aggggcagaa tcctgaaaaa
     3001 tgggatcatt tcggagtcag aagtatgacg gaacgggcga gaatgttagg cggaaacgtg
     3061 cagtgggtgt cgaaacccgg caccggcacg aaggttgaaa ttttcattcc tctcgtatta
     3121 aaggaaaagg tgccttatgc ccaaaacgac taaagtcctc attgttgatg atcatcgtgt
     3181 ggtgcgtgaa gggttatgtg cgattctgga aaccaaggac gatatccagg tcgtggggga
     3241 agcaaaagat ggaggagagg ctgtcgaaca aacccgactg cttatgccgg atgtgattct
     3301 catggatgtc agtatgccgg gcatgacggg ggtggaagcc acgcgaatta ttaaacggga
     3361 atttcctcac atcggggtgg tggcccttac gatgtacgaa gaacagcaat acatttttga
     3421 tctggtgcga gccggagcca ccgggtattt actcaaagat agtgattcgg cgcaaattgt
     3481 ggctgctatt cggactattg cccgtggcga gtctctgata catccatccg tcgccagtaa
     3541 gattctggtc gagttttctt tgttgtctga aggcaaaggc aaaaaacggg gaattttgga
     3601 gcacgatctc accgaccgtg aaatcaccgt gttgcaatta gtggcggatg gaaaaaccaa
     3661 taaggaaatt gcgaacgtcc tggatctcag tgaaaagacg gtcaaaaatc acgtccggaa
     3721 tatctttcat aaactgcatg tgtttgatcg aacacaagcg gccatcctgg ccattcgcaa
     3781 aggcatcatc gaactcgaac ctcgcaagat gtaagccgcg ccgcacgtcc atttcgttgt
     3841 ctcctttcgc attgttctct cgtggcgcgg aaattgcggt ttcttcgttt taccttgccc
     3901 gcctattttc gtcagtgcta acatgacagc attgggaagt ttatcaagga ggagtgaatg
     3961 caatgg
//
LOCUS       DATIAL010000100         9099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_340513, whole genome shotgun sequence.
ACCESSION   DATIAL010000100 DATIAL010000000
VERSION     DATIAL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340513"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..955)
                     /locus_tag="VLA60_03315"
     CDS             complement(203..955)
                     /locus_tag="VLA60_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08415.1"
                     /translation="MRVSRIRLSLLVTVFLCLSTLTGCLGKREWQYPPASTGSFLNEK
                     AAAPIPAKAIVLPLEDLRGNEVKEEYWKAAIPLVPYGDTRYQRPEAAPNPEEVDIVKF
                     DPTNDLAQSIVAELKHAEIFSSVDFAKDEGQQPADLVFRGRLRSTDWNRRLYSYLLAP
                     VGVVFWMLGLPMSETTTALELDLRLTPLNDPSKVLWNMTMEFEGKQLDGPYYGLENAV
                     ESYPEAMQEALKPAIADLVGLANEDPTRLMPR"
     gene            complement(1277..1897)
                     /gene="thrH"
                     /locus_tag="VLA60_03320"
     CDS             complement(1277..1897)
                     /gene="thrH"
                     /locus_tag="VLA60_03320"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HSF08416.1"
                     /translation="MAQPVVTCLDMEGVLFPEIWLAVADKVGIQELRLTTRDVADYDV
                     LMKKRLEVLKMHRITLRDIQEVASTISPLPGVPDFIAWLRERCQIIILSDTYYEFVGS
                     LMKKLEFPTIFCHTLGVDSHGFITDYFLRQHDQKRHAVSAMKGIGFRVLAGGDSYNDV
                     SMLKEADAGFFFCPPDSIVQEFPQFPVARSYAEFQEHLGRAGGFPS"
     gene            complement(2066..2713)
                     /locus_tag="VLA60_03325"
     CDS             complement(2066..2713)
                     /locus_tag="VLA60_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSF08417.1"
                     /translation="MTTWRAEVLFFDFDGTLIDSKIDIATSVNLTLRDLGLALRSREE
                     IFSFVGDGVKRLLRLSVGEENLDQYEKALEIFRKHYLEHCVETTRFYPGIWEVLHHYQ
                     DRRKAIVTNKSLEYTLSIVDGLHAQDLFHHVEAPRDTMELKPEPVMLLRALEGLGVDP
                     SDTVMIGDSTNDVRAAQAAGIRACAVGYGYGNREKVAALRPDFYCENPKDLLGLF"
     gene            complement(2710..3285)
                     /locus_tag="VLA60_03330"
     CDS             complement(2710..3285)
                     /locus_tag="VLA60_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08418.1"
                     /translation="MSLFSWFIRVLLCVLGIVVFSASSLQAIGGHITEDPTKILQKYL
                     SLDKKGVRLEAHSWQVVRPFVAWLEEPVWGHVVVISRYEVVDDVSQWEVINGLEAKIP
                     VIFEVLGTMHWERATFVTNPQTEIQYFHLKAVGDRWQIVGPQLPPHVGRQRLVDFVRW
                     AELNESGPERKMLLNSLIQQLEFTNEKDVQK"
     gene            complement(3348..3824)
                     /locus_tag="VLA60_03335"
     CDS             complement(3348..3824)
                     /locus_tag="VLA60_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08419.1"
                     /translation="MAERILVVDDDKGVREALSEFLLSLGYTVVMAENGEEALKQYRK
                     GDFDVIMADLIMPNMDGMELLRRIRDVKNDEVIFLMITGHPSIGTAVEAINRGADDYI
                     TKPFHLEDVRLRVTKALEKQTLKGRLKTAQGLAWGLMLSIPLWLLLGIILVILFKG"
     gene            complement(3886..4575)
                     /locus_tag="VLA60_03340"
     CDS             complement(3886..4575)
                     /locus_tag="VLA60_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HSF08420.1"
                     /translation="MMSLPFLRGAIYLGIICFMMPVSATSAASVSVMEDIVAKVDARY
                     AQTKDLQADFVQETILEGFTTGFTSSGRLYLKKPGLLRWDYLHPSEEQIYVNGDQVMM
                     YVPEHQQVVKGALTQMAASKGPLALLQGAGNLSQQFTILESADGSKDNEKFPSLTLVP
                     KPVDQTPPTIKKIVLKLFPDTYFIQGITLFEVSGNISHVMFDHIQANTGLSTSQLTFD
                     VPPDVVVVELP"
     gene            complement(4572..6953)
                     /locus_tag="VLA60_03345"
     CDS             complement(4572..6953)
                     /locus_tag="VLA60_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HSF08421.1"
                     /translation="MSVFPSSKSKPTKDQPKGFSVLTTQVVGILVATLGILGLLSLAT
                     FSPSDPILFAEQPSSNSIPENAVGLIGATLAFSLLTMAGGGAYMVPCLLIVLGLSVLW
                     GEKLRITSGSLLGSLIGVLSFSALLHLRFSAGPQVYGINVLGVSQGGVVGQWVAAGLG
                     SYLAVTGATIILAALLLIAFLLLVPVSIGQIVRTLLNFMGRLKNTVVESVRERDWWVS
                     NYQPKMNKSIRINRQLAARGGIGAMVLETLEPKSKLSKIKRDDLPATIEITPTLPLVE
                     EVDDSFISRKKISKSYQMPSPTDLLDTHAARAAHQTDAVLKSQSEVLVNTLASFGIEG
                     GVTDVHPGPVITMYEFAPGPGIKVARIVNLADDLAMALKALKVRVVAPLPGKSTVGIE
                     VPNPQRETVGLKEMLTSEAFTRARSKLALALGKDIFGRPVVTDLRTMPHLLVAGATGS
                     GKSVGLNCLLLNILFTAHPDEVKLLLIDPKVLELQVYDGIPHLIRPVITNPKEAARGL
                     GWVVQEMERRYQVLAELGVRNIDAYNKKVTDSQETGSPLQTVKKLSKGQSMDVEEGAP
                     LLESSQEERVLLPYIVVVIDEFADLIMVAPKDIEDRIARLAQMARASGIHLVLATQRP
                     SVDVVTGLIKANFPARIAYQVSSKIDSRTILDANGAESLLGKGDMLFLSSGTGGINRL
                     HGPYVSDEEVRGVVEWVKSQATPVYDPVALESVQEPSVDEQDRDETYERARELVMTTG
                     QASASFIQRRLRVGYPRAARMIEQMEEEGLVSAPGRDGRREVLARSTAIAEIG"
     gene            complement(7034..7849)
                     /gene="rsmA"
                     /locus_tag="VLA60_03350"
     CDS             complement(7034..7849)
                     /gene="rsmA"
                     /locus_tag="VLA60_03350"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HSF08422.1"
                     /translation="MSPLPPYPRKRLGQHFLIDPNLLRKIINLADLQPDDYVCEIGPG
                     GGALTRLLCQSAKQVLALEVDPRMVDFLKEEFAEFSNLDIQEHDALRFPFDQLPEPAV
                     VVANLPYNISTPIMFRLLEARPKIRRMVLTVQLEVAKRLTAKPNTQDYGVLSVMAQRL
                     ADVRFGFPVSRKCFRPVPDVDSGVVRLDIRVHQTFSAQEADAFALVVRAAFGQRRKTL
                     LNALRGAGFSLICLESAQERTGIALTRRAETLSVGEFQNLSEALNNPMLPSAS"
     gene            complement(7908..8225)
                     /locus_tag="VLA60_03355"
     CDS             complement(7908..8225)
                     /locus_tag="VLA60_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08423.1"
                     /translation="MMTDQTSELLAYIQSQIEEITTIHAQAEKALNAVQGKDHVTKWK
                     KKVVDGLKPYVSEAYFQHITKEWLETTYFVGDVFDELADEVDMCRRHLKKLVKDIQTT
                     GIP"
     gene            complement(8287..>9099)
                     /gene="fmt"
                     /locus_tag="VLA60_03360"
     CDS             complement(8287..>9099)
                     /gene="fmt"
                     /locus_tag="VLA60_03360"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HSF08424.1"
                     /translation="VQPEKMKDPKLLEILRAWEPEVVVVAAFGRILPKPILDLPPKGC
                     LNVHGSLLPKYRGAAPIQWAVIQGEVKTGVTIMLMDEGMDTGAILQQEEISIGPDETS
                     GELAPRMAQFGGALLVRTLRGWVTGTIPPIPQNDSAATMAPILTKEDGLMYWDRPART
                     LANRIRGLSPWPGVYTFLEGERWGIWKVQVEDQEPSIRHTALDISQVPGTITAVTKQA
                     IRVQTGQGRLNLLEIQPENKKRMHVSDYIAGHRVTIGMTFSMGKQGDCGVIV"
BASE COUNT         2518 a         2401 c         1971 g         2209 t
ORIGIN      
        1 cctgaaatct tgaatgaaag aaaaatatct catgaattca tgagagacaa caacgaacgt
       61 ccttgcaaga tgtcagtgtt tcctaatcat tatcggccgt tttttcaatt cctgaagctt
      121 aaaataatct tctgcttacc aaatatcaac cagatatttc atattttttc acgattcttc
      181 acatggcaat aaattcaatc acttagcgtg gcattagccg ggttgggtcc tcgttggcca
      241 agccaaccaa atctgcaatt gcaggtttca aggcctcttg catagcctca ggataacttt
      301 cgacagcatt ttcaagacca taatatggtc catcaagctg cttcccttcg aattccatgg
      361 tcatattcca taacactttc gatggatcat ttaggggggt taatcgaaga tcaagctcca
      421 gagcggtggt ggtttcactc atgggaagac ctaacatcca aaaaaccact cccacgggag
      481 ctaatagata cgagtacaat cgacggttcc agtcagtaga acggagacga cctcgaaaga
      541 ccaaatccgc cggttgttgt ccttcatctt tggcaaaatc aaccgaggaa aaaatttctg
      601 catgctttaa ttccgcaacg atggattgag caagatcatt agtcggatca aatttgacga
      661 tatcgacctc ttcagggttt ggtgcggcct cagggcgttg atatcgtgtg tccccatagg
      721 gaaccagagg aatagcggcc ttccaatatt cctctttgac ctcattcccc ctcaaatctt
      781 ctaagggaag aacaatggct ttagccggga ttggcgcggc ggctttttcg ttcagaaatg
      841 accccgtaga ggccgggggg tattgccact cccgtttccc aagacaccct gttagggtag
      901 aaagacagag aaataccgtt accaatagag acaggcgaat gcgagagacc ctcatgtaaa
      961 tgctcctttc aagtttgtca gtgctcttgg cataaaaact ttgcagccaa cagtcccaga
     1021 ggatctagaa aaatttctgg caattttttg aggattacat ctcttttctt caaaaaatga
     1081 ttctttgatc ctttttcgct ttatcatcag tacttttctt cagccatcta taaaacgcca
     1141 tacatctagc aagtacgatt tggaaacgca acaatggttc ccctcagaaa accctccata
     1201 tattttggca ttagccagag gcctcttgaa cgaaaacacg ggggaaggaa tacaaaccta
     1261 cctcagcatg accacgtcag gaagggaagc cgcctgcccg accaaggtgt tcctggaatt
     1321 ctgcatacga acgagctacc gggaattggg gaaattcctg gacaatcgaa tcaggaggac
     1381 agaaaaaaaa tccggcgtcg gcctccttta acattgaaac atcattatag gagtctcctc
     1441 ccgccaacac tctgaacccg atacccttca tcgcactgac agcatgtcgc ttttgatcat
     1501 gttggcgtaa gaaataatcc gtgataaaac catgggaatc aactcccaat gtatggcaga
     1561 aaatcgtagg gaattcgagc ttcttcataa gtgatccgac aaactcataa tacgtgtccg
     1621 acaatataat aatttgacat cgttcacgca gccacgcaat aaaatcagga acgccaggga
     1681 ggggagaaat cgtactggca acttcctgaa tatcccgcag agtaatgcga tgcattttca
     1741 ggacttccag tcgttttttc attaagacat catagtccgc aacatccctg gtggtcagac
     1801 gtagctcttg gataccaact ttatccgcca cagctagcca gatctcagga aataacaccc
     1861 cttccatatc caaacaggta acaaccggct gcgccatcaa aacctcttct cgtgattaat
     1921 attcaaaaat gttcctagcc atcaaaaata tcaatggatc ataaaagaat tcccgggtta
     1981 gccacaacta gaaaaatcac agggcgacca agcccctttt gtcggatgct gccactcgca
     2041 gacaatgaat tagaggtggg atgaattaaa acagccccag aaggtctttc ggattttcac
     2101 aatagaagtc aggccgtaat gctgcaactt tttctcgatt cccataccca taccccacgg
     2161 cacaagctcg aatacccgcc gcctgggctg cccgaacatc attcgtacta tccccaatca
     2221 tcaccgtatc agaaggatcg acgcccaatc cctctagtgc ccgcaacaac atcacaggct
     2281 caggctttaa ttccatcgta tcccgaggag cttcgacatg atgaaagaga tcctgtgcat
     2341 gtaacccatc cacaattgaa agggtatact ctaaggactt gttggtcaca atagcttttc
     2401 ttcggtcctg ataatgatga agcacttccc aaatcccggg ataaaaccgt gtggtttcca
     2461 cgcaatgctc tagataatgt tttcgaaaaa tctccaaggc tttttcatat tgatcaagat
     2521 tttcttcccc caccgataac cgcaatagcc gcttcacccc atcacccaca aaactaaaaa
     2581 tttcttcccg tgaacgtaag gccaagccca aatccctcag cgtcaggttt accgaggtgg
     2641 caatatctat tttcgaatcg atcaaagttc cgtcaaagtc aaagaacaac acttccgcac
     2701 gccatgtagt catttctgaa catctttctc atttgtaaac tctaattgtt gtatcaaaga
     2761 attgagtagc atttttcttt ccggcccaga ttcatttaac tccgcccaac gaacaaagtc
     2821 gaccagacgt tgacggccca catggggagg caattgaggc ccaacaattt gccaccggtc
     2881 cccaaccgcc ttcagatgaa aatattggat ttcagtttgt ggattggtca caaacgttgc
     2941 gcgctcccag tgcattgttc ctaacacctc aaagattacg ggaattttgg cctctagccc
     3001 attaataact tcccattgcg atacatcatc caccacctca tatcgagaaa tcacgactac
     3061 gtgcccccac accggttctt ccagccaagc cacaaagggc ctcaccacct gccaggaatg
     3121 cgcctccaac cgaacgcctt ttttatcgag ggaaagatat ttctgtaaaa ttttggtggg
     3181 atcttcagtg atgtggccgc caatggcctg aagagagctt gcactgaaca ccacaatacc
     3241 caaaacacac agtaagaccc gaatgaacca cgaaaacagg ctcacaatga atttgggatt
     3301 ggcgattgag ccatgaccac acaagaatca ctggaagaaa cagcgactta tcctttaaac
     3361 aggataacca gaattatccc caataagagc caaagaggaa tggacaacat taaaccccag
     3421 gccaagcctt gggctgtttt taagcggcct tttaaggttt gtttttccaa cgctttcgtt
     3481 acccgtaatc tgacgtcctc gagatggaag ggtttggtaa tataatcatc ggccccacga
     3541 ttaatagcct ccacagctgt gccaatggag ggatgtccag tgatcatcag aaaaatcacc
     3601 tcatcatttt tgacgtcccg aatacgcctc aacaactcca tgccatccat attgggcata
     3661 atcaagtccg ccatgatcac gtcaaagtca cccttccgat attgtttcaa ggcctcttca
     3721 ccattttcgg ccatgacgac ggtataaccc aacgagagca agaactctga caaggcttcc
     3781 cgaactccct tgtcgtcatc cacaaccaag atgcgctcag ccatttcttt ccaccttttc
     3841 cttatcagag aacaccatct aaaacgaccg acctgaatgt tgaaatcagg gtaattcgac
     3901 caccacaaca tccggcggaa catcgaaagt cagctgacta gtcgaaagac ccgtattcgc
     3961 ctgaatgtga tcaaacatca catgagagat attcccgctc acctcaaaca aggtaatacc
     4021 ctgaataaaa tacgtatcag gaaagagttt gagcacaatt ttcttgatag ttggaggggt
     4081 ttggtcaaca ggcttaggga cgagggtgag actcgggaac ttttcattat ctttcgagcc
     4141 atctgcggat tccagaatcg taaattgctg agatagattt cctgccccct gcagaagagc
     4201 gagaggaccc ttggacgcgg ccatttgggt aagcgcccct ttgaccacct gctgatgctc
     4261 agggacatac atcatgacct gatcaccatt cacataaatc tgctcttccg atgggtgaag
     4321 ataatcccat cgaagtaatc caggcttttt taaatagagc cgccctgaag aggtaaaccc
     4381 cgtcgtaaat ccctcaagaa tggtttcctg gacgaagtca gcttgcaaat ctttggtctg
     4441 agcataacga gcgtcaacct tcgcaacgat atcttccatg acggaaaccg atgcagccga
     4501 ggtagcggat acaggcatca taaagcaaat aatgcctaga tagatggccc ctcgcaggaa
     4561 cggcagtgac atcatcctat ctccgcaata gccgtacttc gagcgaggac ctctcgtctc
     4621 ccatcacgcc ctggagcact caccagacct tcctcctcca tttgctcaat catccgggct
     4681 gctcggggat accccacacg caagcgacgc tgaataaacg aggctgaggc ctgtcctgtg
     4741 gtcatcacca attctcttgc ccgctcatag gtctcatcgc gatcctgttc atccaccgag
     4801 ggttcctgaa cggactctag ggccaccgga tcatagacgg gagtggcttg ggatttcacc
     4861 cattccacta ccccacgcac ttcctcatcc gagacataag gaccgtgcag tctgttaatt
     4921 ccaccggtgc cggaggataa aaacagcata tcaccttttc ccaaaagact ttctgctcca
     4981 ttcgcatcta aaatggtccg ggaatcaatt tttgaagaaa cctgataagc gatacgtgca
     5041 ggaaaattag ccttgatcaa tcccgtcacg acatcaaccg atggtcgttg agtcgctaag
     5101 acaagatgaa tgcctgacgc acgggccatc tgagcgagtc gcgctatgcg atcttcaata
     5161 tccttaggcg ccaccataat cagatcggca aattcatcaa tgaccactac aatgtaaggc
     5221 aacagaaccc gctcttcttg cgaagactcc agcagggggg ccccttcctc cacatccatc
     5281 gattggcctt tgctcagctt cttcacggtc tgaagtggag aacccgtctc ctgagaatcg
     5341 gtaacttttt tattataggc atcaatattt cgaaccccca attccgctaa cacctggtaa
     5401 cgacgctcca tttcttgtac gacccaccct aacccacgag ccgcttcttt gggatttgtg
     5461 ataaccgggc gaatgagatg gggaatccca tcgtacacct gcaattccaa cactttggga
     5521 tcaattaaca ggagttttac ttcatcggga tgggcggtaa ataggatatt caataacagg
     5581 cagttcagcc ccacactttt ccctgacccc gtagcacccg ctaccaataa atggggcatg
     5641 gtacggagat ccgtcaccac cggtcgccca aaaatatctt tccctaaggc caaggccagc
     5701 tttgagcggg cccgggtgaa agcctccgat gtcaacattt ctttcaatcc aaccgtttcc
     5761 cgctgaggat tgggcacttc aataccaacg gtggattttc ctgggagagg agccaccact
     5821 cgaactttta aggcttttaa ggccatggct agatcatcag ccaaattcac aatgcgggcg
     5881 actttgatgc cgggaccggg agcaaactca tacatggtaa tcaccggtcc aggatgcaca
     5941 tccgtgacac ccccctcaat cccaaaactt gctaacgtgt tcacaagaac ttcggactga
     6001 gattttaaca ccgcatcggt ctgatgagcc gcccgcgcag catgagtatc cagcagatcg
     6061 gtgggggaag gcatttgata ggactttgaa attttcttgc gagagataaa gctgtcgtcg
     6121 acttcttcaa cgagtggaag agtaggcgtt atctcgattg tagctggcaa atcatctctt
     6181 tttatcttgg ataacttgga cttgggttcc aaagtttcca ataccatcgc accaatacca
     6241 ccacgagcag ccaattgacg attaattcga atcgatttat tcatttttgg ttggtaattg
     6301 gagacccacc agtctcgttc cctcaccgat tccaccacgg tatttttgag gcgccccata
     6361 aaattcaaga gagttcgaac aatttgccca atcgataccg gcacaagcaa aagaaaggca
     6421 atgagtaaca gtgccgccaa tatgatcgtg gcaccggtga ccgccagata cgacccgagt
     6481 ccggccgcaa cccattgtcc cacgactcca ccctggctta cccctaagac attaatcccg
     6541 tatacttgag gcccggctga aaaccgaaga tgtagcaggg cgctgaaaga tagaacacca
     6601 atcagcgatc ccagaagact tccggacgtt atacgaagtt tttcgcccca aaggacgctt
     6661 aaacccaaca cgatgagcaa gcatggcacc atataagccc cccctcccgc catagtgagg
     6721 agggaaaacg cgagagtagc cccgatcaga cctacagcgt tttcgggaat tgaatttgag
     6781 gaaggttgct cggcaaaaag gatcgggtcg gaaggagaaa aggttgccag gctgagcagc
     6841 cctagaatac ccaaagtagc gactaaaatt cctacaactt gtgtggttag gacagaaaat
     6901 cccttgggtt ggtctttagt tggcttgctc tttgaactag ggaagacact catcaaaaaa
     6961 tcctaccata agtgtttcta tcgagcaaac aacccttacg catatgacaa ggttatacaa
     7021 aatcctgggg atgttacgaa gcggagggca gcattggatt attgagtgct tcacttaaat
     7081 tctgaaactc ccccaccgac agcgtttctg ctcgcctagt taatgcgata cctgtgcgtt
     7141 cttgagcact ttctaggcag atcagagaaa aacccgctcc tcgtagtgcg ttgaggaggg
     7201 tttttcggcg ttgcccaaat gctgcccgca ccaccaaagc aaacgcgtcg gcttcctgag
     7261 cagaaaaagt ctgatgaaca cgaatatcca atcgtaccac tcccgaatcg acatcaggaa
     7321 cggggcgaaa gcattttcga gacacgggga aaccgaaacg gacatcagcc aagcgttgcg
     7381 ccatgacgga tagcactcca tagtcctgag tattgggctt ggcggttaac cgcttggcga
     7441 cttccagttg cacggttagg accattcgac gaatcttggg ccgggcttct aacaaccgaa
     7501 acataatggg cgttgaaatg ttatagggca gatttgctac caccactgca ggttctggga
     7561 gttgatcaaa gggaaagcgg agcgcatcat gttcttgaat atcaaggttc gaaaattcag
     7621 caaattcttc cttcaagaaa tctaccatcc ggggatcgac ttccaaggct aacacctgct
     7681 tggccgattg gcacaacaat ctggtcaacg cgccgccacc cggtccaatt tcacagacat
     7741 aatcatcagg ctggaggtca gccagattaa taatttttcg gagcaaattt gggtcaataa
     7801 ggaaatgctg accgagccgt ttgcgcggat aaggagggag gggtgacatt ataggcaggg
     7861 gtaaaaatga gaggggtcaa accggtgctt catatgggaa gaaagcatca ggggatgccg
     7921 gtggtctgaa tgtccttaac taacttctta agatgtcgac ggcacatgtc cacttcatcg
     7981 gctaattcat caaacacatc gcccacaaaa taggtggtct ccaaccattc cttggtaatg
     8041 tgctgaaaat atgcctcact cacgtagggt tttagaccat caaccacttt tttcttccat
     8101 ttggtgacat ggtctttccc ctgcaccgca ttgagggctt tctccgcctg tgcgtgaata
     8161 gtagtgattt cctctatttg actctgtata tacgccaata actctgaggt ctgatcggtc
     8221 atcatagtat cctttttcac caaaacgggt catacgaaaa ggcctgacac cagaaccctg
     8281 ccggcctcac acaattacac cacaatcgcc ctgcttcccc attgaaaagg tcatgccgat
     8341 ggtaactcga tgtccggcta tatagtcaga aacgtgcata cgttttttat tttcaggttg
     8401 aatctccaaa agattcaacc ttccctgacc ggtttggaca cggatggcct gcttcgtgac
     8461 ggcagtaatg gtgcctggga cctgagaaat gtcaagggca gtgtgccgga tacttggttc
     8521 ttgatcttca acttggactt tccaaatccc ccatcgttca ccctccagga acgtatacac
     8581 accaggccag ggcgacagtc cgcgaatgcg attggcgagg gttcgcgccg gccgatccca
     8641 atacatcaac ccatcttctt tggttaaaat gggcgccatg gtcgccgccg aatcattttg
     8701 ggggataggg ggtattgtgc ccgtgaccca tccacgtaga gtcctgacca gtagggctcc
     8761 gccaaactga gccattcgtg gagctaactc tcctgaggtt tcgtccgggc caatagaaat
     8821 ttcttcctgt tgaagaatgg cccccgtgtc catcccttcg tccataagca taatggtaac
     8881 tccggtcttc acctctccct ggattacagc ccattgaatg ggagcagccc cccgatattt
     8941 cgggagaagg gaaccatgaa cattcaggca tcctttcggg ggaagatcaa gaatgggttt
     9001 gggtaaaatt cgaccaaacg cagccaccac taccacttca ggctcccatg cgcgaagaat
     9061 ttcaagtaat ttgggatcct tcattttttc aggctgcac
//
LOCUS       DATIAL010000101         6018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_662725, whole genome shotgun sequence.
ACCESSION   DATIAL010000101 DATIAL010000000
VERSION     DATIAL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662725"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..2488)
                     /locus_tag="VLA60_03365"
     CDS             complement(266..2488)
                     /locus_tag="VLA60_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSF08425.1"
                     /translation="MNNHKCAIFPNVIIRVMIFTLTGFFLFQPPFSRADEQIETLPTV
                     TVEGTRIENIDAVKEELARRPSSTILIPEKEIEESRAFNLTDVLQFAPGVRFQSRFGA
                     DEGQFQIRGTSLRNNFHHRGINILINGIYFGDADGFSDFESIDLMAYERIEVYKGSNA
                     LRYGANTLGGAINFVPRTGYNASLFQTRGMAGSFGLYSGQVSSGQVTKPFKVGTMDTT
                     ADYYISASGNHQDGFQDNSQQARGRLNANFGFQIGDHQEVRAYILGAEVSEALPGSIT
                     QAQLDSNRRQANTLGGISPFSCGPNEACKWGRYYSYIRVGAAYRNEFAAHQFFEIIPY
                     YSYQYLDHPIFQTIVQDNYNVGGEIRYINSHPIFGHENKFLIGNQPRYGDTNQRRFVN
                     NLGKRGTQTQNLTLETTNLSTYLEDQFNVTDDFLVIAGGRWEYGIRQGRNQLYSPFTG
                     NLTTNDTGVRHFNALIPKMGLVYQTTPTSQIYGNVSRGYEPPVNVELTSSINPDGSIP
                     TSPFINLDAQRAWQFEIGTRGSTADNRYTWDLTFYNLEMQKEILTFNVNNQPTYQNAK
                     GTRHTGIEAGGSMVVANGLLAQGPKNQTDTITVRTAYTWSLFKFTDDVYGGGENGTQK
                     FLVAKDGNRVPGAPEHQLSGEIRYDHPVGFFIAPNFEWIIAGYYVDSLNTAKNSAYFI
                     VNLKAGYNYSKNLSIFAEGRNLSNQTYAGAVVVNDSLGRYFNPGQGISGFGGIEWKFN
                     "
     gene            complement(2601..2945)
                     /locus_tag="VLA60_03370"
     CDS             complement(2601..2945)
                     /locus_tag="VLA60_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08426.1"
                     /translation="MPNKPSSLPIIPRQLTLPSIILAVVYLALVSIATYCVASHEIQH
                     SPINHHSKNTSSHSLLCSWACQVSSKANSADTTHKALLPLTLFIVGVFFLCFVIPTQA
                     TLSPILARGPPV"
     gene            complement(3006..4502)
                     /locus_tag="VLA60_03375"
     CDS             complement(3006..4502)
                     /locus_tag="VLA60_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08427.1"
                     /translation="MSETNRGAIVEAERQWGLSKQAAHAMLDMTSLFLIVGLGLVGCS
                     TTTHPPPTPSFESVHNGNAAKTYAFINGQWFDGQGFTRDTWYAVQGSLTRFRPESVEQ
                     TIDLKGGFVVPPFGEAHTHNVEGAWNIDQVISHYVRDGVFYVKNPNDISEFVEQIRGK
                     LNTPTTIDVVFAHAGLTGPNGHPIDLYENMLRTHRYEPVIGKREKAWFNGRAYFPISS
                     LKNFEEQWPTIMATKPDFLKLYLADSEHFENEPHSVTHGFRKGLGPQLIAPIVAHAYQ
                     QGLRVSAHIETAADFRAAIQGQVDEIAHLPGWYLPSPAQRSAVLLTQEDAQLAAAHRT
                     VIITTTVAVHFHPPGHHQSDSEAHPHSPANTGDHKGQSTENIREVARAIQTDNIRLLQ
                     QARVLIAIGSDHAETALSEALHLHQMGIFDNLTLLKMWSETTPQTIFPSRKIGRLDEG
                     YEASFLVLQGNPIEDFKQVRNIIFRLKQGSPLSWSEPQNADFTGKHAH"
     gene            complement(4593..5180)
                     /locus_tag="VLA60_03380"
     CDS             complement(4593..5180)
                     /locus_tag="VLA60_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSF08428.1"
                     /translation="MIQLLMGGGWMMIPILGCSLLALTISIERGFQFRSLRFSRLADR
                     MVKLVEQGKWTDALALAEQRQSPTLRVLAAGIFHRDQQPDKAMEATGIAEISRLKRGL
                     SVLDTVITLGPLLGLLGTIIGMIDSFGIMSKTGLGNPHAVTGGVAEALICTAAGIGVA
                     VITLIPYNFFLSLVERETERIEQYATKLQSFLGHS"
     gene            complement(5208..>6018)
                     /locus_tag="VLA60_03385"
     CDS             complement(5208..>6018)
                     /locus_tag="VLA60_03385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSF08429.1"
                     /translation="AAILAALAYIPSPPTFKDPTPTVQVTLVPALQEISPTPQPVTKP
                     LTPTTPHFAQPTPPPPRTEPTPSMPAPPMQASIKQTPSKPLTPPPPLPQAQPMLKDTR
                     TEQAMKSRQMMKMAAPSQHQQRPPLSTAPPQQEQPTARTPLPMNNREPRAQHTLPPPP
                     SPAARPALKTPPPMAAGSSTSKPKIVASSKPLYPRVARESGWEGTVIVRTLITADGAP
                     SQVEIRKSCGHEALDMAAQEAIKNWKFLPAKDGNIPIAKWVDIPIKFDLNS"
BASE COUNT         1345 a         1427 c         1692 g         1554 t
ORIGIN      
        1 aaagaacacc aagagaaaga acatcgtgtc gatcatcgga atgatttcga tacgggcttt
       61 cttttgtgtg gtggtagata gtctcatgaa aatctcattg cccagttcgt tgtggtaaac
      121 aggttattag ggtgaattgg aaattaaaaa aatcttcttc ccttaagttg acggcctatt
      181 gagcgtcaca cctagaataa cgaactaggt gtgacaacct caatatattc catatatccg
      241 cacgatggtg tgtagcatga atcgattaat tgaatttcca ttcgattccc ccaaacccgc
      301 tgatcccctg gccggggttg aaataccggc ccagactgtc attgacgacg acagcacccg
      361 cataggtttg attggacagg tttcgtccct ctgcgaaaat ggacaggttc ttactgtaat
      421 tatagcctgc tttcagattg acgatgaaat aggccgagtt cttggcagtg tttaagctgt
      481 ccacgtaata tccagcgatg atccattcga aattcggcgc gataaaaaat cccactggat
      541 ggtcatagcg tatttccccg gaaagttggt gctcgggcgc gccgggaacc cggttgccgt
      601 ccttggccac caagaatttt tgagttccat tttctcctcc gccgtacaca tcatctgtga
      661 atttaaataa cgaccacgta taggcggttc gaacggtgat cgtgtcggtt tggtttttgg
      721 gaccctgagc cagcaatccg ttggccacca ccatgctgcc tcctgcttca atgccggtgt
      781 gtcgcgttcc tttggcattt tgataggtcg gttggttatt gacattaaat gtcaggattt
      841 ccttttgcat ttccaggtta tagaaggtga gatcccaggt ataacggtta tccgccgtgc
      901 tgcctcgtgt gccgatttca aactgccagg cccgttgtgc atccaggttg atgaaggggc
      961 tggtggggat tgagccgtcg gggttgatcg atgaggtcaa ttcgacatta acggggggtt
     1021 cgtacccacg gctgacgttt ccgtaaattt gcgaggtcgg ggtggtttgg tacacgagcc
     1081 ccatctttgg gataagagca ttgaaatgac gtacgccggt atcgttggta gttaaattcc
     1141 ccgtaaaggg actgtagagt tggtttctgc cctgccggat cccatattcc cagcgtccgc
     1201 ctgcaataac aaggaaatca tcggttacat tgaattggtc ttccaggtag gtgctgagat
     1261 tggtcgtctc caacgtgaga ttttgggtct gagttccgcg tttccccaaa ttattgacaa
     1321 aacgccgttg attggtgtcc ccgtatcgtg gctgattgcc gatgaggaac ttgttttcat
     1381 gcccgaaaat gggatgtgaa ttgatgtatc gaatctcccc tcccacatta tagttgtcct
     1441 ggacgatcgt ttgaaaaata ggatgatcca ggtactggta ggaataataa gggataattt
     1501 caaaaaactg gtgggcggcg aattcgttgc gataggcggc cccgacacgg atataactat
     1561 agtatcgccc ccatttgcat gcctcgtttg gcccgcagga gaacggactg attcccccca
     1621 aggtgttggc ctgtcttcga ttggaatcca attgcgcttg ggtgatcgac ccggggaggg
     1681 cttctgacac ctcggctccc aggatatagg cgcgcacttc ctggtgatca ccgatctgaa
     1741 atccgaagtt ggcatttaac cgtccgcgtg cctgctggct attatcctgg aagccgtcct
     1801 gatgattgcc cgacgcgctg atatagtaat cggctgtggt gtccatggtg cccaccttaa
     1861 atggtttggt cacctggccg ctggagactt gtccggaata gaggccgaaa ctcccggcca
     1921 ttccacgcgt ctggaagaga gaggcgttgt agcctgtccg gggcacaaaa ttgatggctc
     1981 ctcccagagt attggctcca taccggaggg cgttggaccc tttgtacacc tcaatacgtt
     2041 cataggccat caagtcgatg gattcgaaat cggagaaccc gtccgcatcg ccgaaataga
     2101 tcccgttgat gagaatgttg attccacgat gatgaaagtt gttcctgagg gaagtccccc
     2161 ggatttggaa ttgtccctcg tcggcaccga accgggattg gaatcgcacg cccggggcga
     2221 attgaaggac gtcggttaaa ttaaacgcac gggattcctc gatttctttt tccgggatca
     2281 ggatggtact ggaaggtcga cgagccagtt cttccttgac cgcatcgatg ttctcgatcc
     2341 gtgttccttc cacggtgact gtcggcagtg tttcaatttg ctcgtcagcc ctgctgaatg
     2401 gaggttggaa tagaaaaaac cccgtcaacg taaaaatcat cacgcgtatg attacattag
     2461 gaaatatcgc acatttgtgg ttgttcatcg gtgcataaat cctttccgga acctatgaaa
     2521 atagatcatg ctcagagcaa acaaaggcct agccctgatg gagaaaaatt atggattggt
     2581 tgggaaacgc gagactttgg ttagacggga gggccgcgag cgagaatcgg actcagggtc
     2641 gcctgcgtcg ggatgacgaa acagaggaag aagactccga caatgaatag ggtgagggga
     2701 agaagtgcct tgtgggtcgt gtcagccgag ttcgccttcg aactgacctg acaggcccat
     2761 gagcagagca aggaatggga actggtgttt ttggaatgat gattgattgg agaatgttgg
     2821 atctcatgag atgccacgca atacgtggct atgctcacca gtgcgagata caccactgcc
     2881 aggatgatgg aggggagtgt aagttgacgc gggattatgg gaaggctcga tggcttgtta
     2941 ggcatgttaa agcttgtatg tcttgtgcct taaaaaatcc cggttatctc agaacgactc
     3001 gcaggttaat gcgcatgttt gcctgtgaaa tctgcattct gaggttcgct ccatgaaaga
     3061 ggggaacctt gcttgagacg aaagataatg tttcgaacct gcttgaagtc ctcaatcgga
     3121 ttgccctgta agacaagaaa gctggcttca tatccctcat ctaatcgtcc aattttcctt
     3181 gaaggaaaaa tcgtctgagg agtggtctca ctccacattt tcaataaggt cagattgtca
     3241 aatattccca tttgatgaag gtgcagggct tccgatagtg ccgtttccgc atggtcgctt
     3301 ccgatggcaa tgaggaccct cgcttgctgc agtaacctga tattatccgt ctgaattgca
     3361 cgggcaacct cacggatatt ttcggttgat tgtcctttgt gatcaccggt attcgcaggg
     3421 gagtgaggat gtgcctccga atcagactga tggtgccctg gcggatgaaa gtgcactgcg
     3481 acggtcgtcg tgataatcac ggtccggtgt gcggccgcta gttgggcatc ctcttgagtt
     3541 aacagcacgg ctgatcgttg agcgggtgaa gggagatacc accccggaag atgggcgatc
     3601 tcatcgactt gcccctggat ggctgcccga aagtcagcgg ccgtttcaat gtgtgcggaa
     3661 actcgtaagc cttgctggta cgcatgcgcg acaatcgggg cgatgagctg aggacccagc
     3721 ccttttcgaa acccatgcgt aacggagtgg ggttcgtttt cgaaatgttc tgaatcggca
     3781 agatacagct tcaggaaatc cggcttggtg gccatgatcg tcggccactg ctcctcgaaa
     3841 tttttcagtg aagagatagg gaagtaggcc cgaccgttga accaagcctt ttcacgttta
     3901 ccaatgaccg gttcatatcg atgggtgcga agcatgtttt cgtaaagatc gatgggatga
     3961 ccgttaggtc ccgtgaggcc ggcatgagca aagacgacat caatggtcgt tggtgtgttg
     4021 agtttacccc ggatttgttc cacaaattca gaaatgtcat ttgggttttt cacataaaaa
     4081 acaccatccc gcacataatg actgatgacc tggtcgatgt tccaggcgcc ttcgacatta
     4141 tgggtgtggg cttcaccaaa cggcggcacg acaaaaccgc ccttcagatc gatggtctgt
     4201 tcaacggact ccggccggaa gcgggtgagt gagccctgca cggcatacca tgtgtcccgg
     4261 gtaaagcctt gcccatcaaa ccattgccca ttgataaatg cataggtctt ggccgcattc
     4321 ccattgtgta ctgattcgaa acttggagtc ggtggcggat gagtggtcgt gctgcatccc
     4381 accaatccta gtcccacaat gaggaacagg ctggtcatgt ccaacatggc atgagccgcc
     4441 tgcttgctca gaccccattg tctttcagcc tcaacgattg cgcctcgatt ggtttcagac
     4501 atggtttgag tgaaccttcc ggtggccgac cgcgatcctg ctgcgttgtt gtcctgggtt
     4561 ttgcatgacc gcacgtattc tgaatgcgag agttaggaat gtcccaggaa cgactggagt
     4621 tttgtggcat attgttcaat acgctcggtt tcacgttcga ccagggaaag aaaaaagttg
     4681 taaggaatga gcgtaatgac ggcaacccca attcctgccg cggtgcaaat gagtgcttca
     4741 gccactcccc ctgtgaccgc gtggggattg ccgagtcctg ttttggacat gatgccgaag
     4801 gaatcgatca ttccaatgat ggtcccaagc agtcccagta agggccccaa cgtgatgacc
     4861 gtatccagga cggacaaccc gcgctttaat cgagatatct cagcgattcc cgtagcttcc
     4921 atggctttgt caggctgttg gtctcgatgg aatatccctg ccgcaagcac gcgcagtgtg
     4981 gggctctgac gctgttcagc caaggccagc gcatcggtcc attttccctg ttcgaccagt
     5041 tttaccatcc ggtccgccaa gcggctaaag cgcaacgagc ggaattggaa tccccgttcg
     5101 atgctgatag tcagagctaa caatgagcag cctaagatgg ggatcatcat ccatccgcca
     5161 cccattagta attgaatcat gtctcggttc ccttcttctc gcgtcgctta actgttcagg
     5221 tcaaatttaa tcgggatgtc aacccatttg gcgatgggga tgttcccgtc tttcgctggc
     5281 agaaatttcc agtttttaat ggcttcttgc gcagccatat ctaaggcttc atgcccgcag
     5341 ctctttcgaa tttctacctg gctcggagcc ccatcggcgg taatcaatgt tcggacaata
     5401 accgtgccct cccaaccaga ctcccgtgcc acacggggat acaatggctt ggatgaggcc
     5461 acaattttcg gtttggaagt tgaggatccg gccgccattg gtggcggagt ttttaatgcg
     5521 ggacgagccg ccgggcttgg cggaggcggc agtgtgtgtt gggctcttgg ttcgcgattg
     5581 ttcattggta agggagttct ggcagtgggt tgctcctgct gcggcggcgc tgttgaaagc
     5641 ggaggtcttt gctggtgttg agaaggcgct gccattttca tcatttgacg actcttcatg
     5701 gcctgctccg ttcgcgtgtc tttgagcatc ggttgtgcct gaggaagagg cggcggagga
     5761 gtcagaggtt tggatggggt ctgtttgatc gatgcctgca tcggtggcgc cggcatggac
     5821 ggcgtaggct cggtccgggg gggcggtggc gttggttgcg caaaatgtgg tgtggttggc
     5881 gtgaggggtt tcgtgaccgg ttgtggggtg ggactgatct cctggagagc cggaaccaaa
     5941 gtcacctgaa ccgttggcgt ggggtccttg aatgtgggag gacttggaat ataggccagg
     6001 gctgcaagaa tggccgca
//
LOCUS       DATIAL010000102         3687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_770729, whole genome shotgun sequence.
ACCESSION   DATIAL010000102 DATIAL010000000
VERSION     DATIAL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770729"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VLA60_03390"
     CDS             complement(<1..1480)
                     /locus_tag="VLA60_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF08430.1"
                     /translation="MSSHFFIDRPIFASVISIVIVVVGLVSLQILPIAQFPEITPPVV
                     QIEADYPGASAEVVAEAVARPIEVQLPGIDNLLYYDSSSTNDGHMTIRLTFEIGTDVD
                     IAQVQTQNRQRLAEPQLPPEVVRQGITVKKVSPDLLGVVALSSSDPRQDTVFLSNFAI
                     LRIVDNIKRLPGVGDALIFGGQNYSMRLILDPIRMAQLNVTPTDIANVVREQNRDFPA
                     GRIGREPAPVGTELTIPVITQGRMSEAKEFEEMIVRAYADGSMVRLGEVAKVELGAQS
                     YDLEGRWNGKPNVFLLTFLSPGANALETMKRIKDEMESLKNIFPAGVSYDIPYDTTRF
                     IEVSIQQVVKTLGEALILVILVVYLFLQSWRATVIPAVAVPVSLIGTFAGMVALGFSI
                     NTLTLFGMVLAIGIVVDDAIVVVENVARHIQNGHPPKEAAKLAMTEVTGPVIALVVVL
                     AAVFLPVAFLGGITGELYQQFAITITLSVVLSGIVALTLSPAL"
     gene            complement(1477..2733)
                     /locus_tag="VLA60_03395"
     CDS             complement(1477..2733)
                     /locus_tag="VLA60_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF08431.1"
                     /translation="MNLAILRLVASPLFNGGVVIRWTIGILLIFLAGCGQGATSPPAP
                     PPPMVEVVTVTTQDLPDEPEFIGQTEAFRPVEIRSQVTGIIKKVFFTEGRNVKQGDRL
                     YLIDPVPFKAAYLSAKARVTQAEARLVQAKQDFARVKPLLGEQAVSQKDVDDALAEGL
                     AAKAALEAAQGDLVKSRFDLSNTLIVAPIAGRISKSRFYEGRLVSAQTDLMTTIDQLD
                     PMYVNVSVPETYLLRRRRELAAHRVQRPDLFQLRGVMTFADGSVYPHEGKLDFADVAI
                     RSETGTLQGRFAFPNPEADLSPGKSYLYPGQFVRIRLKGYIRTDAILIPQRAVQQGPK
                     GTFVNVIGPDDKVEVREVDATGWRGGDWLIEEGLQPGERVVVEGFHRIQPGVQVNPVP
                     FGNGEVTSESPSHKNPTSSEITQENS"
     gene            complement(3031..>3687)
                     /locus_tag="VLA60_03400"
     CDS             complement(3031..>3687)
                     /locus_tag="VLA60_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HSF08432.1"
                     /translation="TIVYQGKVAHRDSAGNGGTIGPGDVQWMTAASGVVHEELHEQAW
                     AREGGTLEMIQLWVNLPRSLKMSAPRYQTVLNEDIPQANLGGQGGVLRVIAGEYEGRK
                     GPAKTFTPVHLYDLKLVAGHRGELNLPVGYNTGLFVLSGGLILNGSHKVGEAEMALCD
                     PQGTKVDLEATEEARVLVLSGEPILEPVARMGPFVMNTEEELVQAVNDYRAGRMGHLD
                     "
BASE COUNT          865 a         1115 c          892 g          815 t
ORIGIN      
        1 agagcgcggg actgagggtc aacgccacga tcccggaaag gaccacggac aacgtaatcg
       61 tgatcgcaaa ttgctgatac agctcaccgg taatgccacc aaggaacgcc acgggcagga
      121 agaccgccgc cagcaccacg accagcgcaa tgaccggtcc ggtcacttcc gtcatggcca
      181 gcttcgcggc ttcctttggc gggtgtccat tctgaatatg ccgggccacg ttttccacga
      241 ccacaatcgc atcatccacc acaatcccaa tggccaacac catgccgaac agagtcaaag
      301 tattaataga aaacccaagc gccaccatac ccgcaaacgt gccgatcagt gacacaggaa
      361 ccgccaccgc aggaatgacc gtggcacgcc agctttgaag aaagagatag accaccagaa
      421 taaccaggat caaggcttcg cccagggtct tcacgacttg ttgaatggac acttcaatga
      481 atcgggttgt atcgtacggg atgtcgtatg agactccagc cggaaaaata tttttaaggc
      541 tctccatctc gtccttgatg cgtttcatgg tttcaagggc attggctccc ggggaaagaa
      601 aggtcaacag aaagacattc ggctttccat tccaccggcc ttcaagatca taggattgtg
      661 cccccaactc aactttggcc acttcgccaa gccgaaccat ggagccatcc gcataagccc
      721 ggacaatcat ctcctcgaat tccttggcct cgctcattcg tccttgcgtg atgacgggaa
      781 tggtcagctc agttccaacg ggggcaggct cacgtccgat ccgaccagcc gggaaatccc
      841 ggttttgctc acgaacgaca ttcgcaatgt cggtgggcgt tacattaagt tgcgccatcc
      901 ggatcgggtc caggatcaac cgcatggaat aattctgtcc cccaaaaatc agcgcatctc
      961 ctacgccggg aagccgttta atattatcca caatccggag aatcgcgaaa ttggagagga
     1021 agaccgtatc ctggcgcgga tcactggaac tcagcgccac cactccgagt agatcaggag
     1081 aaaccttctt cacggtgatg ccttgccgga ctacttcggg gggaagctgt ggttcggcca
     1141 gccgttggcg gttttgagtc tggacctgtg cgatatcgac atccgtgccg atttcaaagg
     1201 tcaaccggat cgtcatatga ccatcgttcg tacttgagga atcgtaatac aagagattgt
     1261 cgatgccggg gagctgcacc tcaatcggcc gcgccacggc ttcggccacg acttctgcac
     1321 tggcgccagg atagtcggct tcaatttgga ccacgggcgg agtgatctcc ggaaattggg
     1381 cgatcggaag aatctggagc gaaaccagcc ccacgaccac gatcactatg gaaatgaccg
     1441 aagcaaaaat aggccggtcg ataaaaaaat gagaactcat gagttctcct gtgtgatttc
     1501 agaagaggtc ggattcttat gactcggact ttccgatgtg acctctccat tcccgaacgg
     1561 caccggattg acctgcactc ccggttgaat ccgatgaaat ccttcgacga cgacccgctc
     1621 acccggctga aggccttctt caatgagcca atcgccccca cgccaaccgg tggcgtccac
     1681 ttcacgcact tcgactttat cgtcgggtcc gatcacattc acaaacgtgc ctttaggacc
     1741 ctgttgaacc gcgcgctgcg gaatcaaaat cgcatcggtc cggatatatc ccttcagccg
     1801 gatacggacg aattgtccgg gatacaaata ggatttgccg ggcgaaagat cggcctcagg
     1861 attgggaaag gcaaaccggc cctgcaaggt tcccgtctcc gaccggatgg ccacatcggc
     1921 aaaatccagc tttccttcat ggggatagac actgccgtct gcgaacgtca tgactccgcg
     1981 taattgaaag agatcgggcc tctggactct gtgggccgcc agttcacgcc tccgtcgcaa
     2041 cagatatgtt tccggaacgg atacattcac atacatggga tctaactgat cgatggtggt
     2101 catcagatct gtctgggcag acaccagacg tccttcataa aaccggcttt tcgaaatacg
     2161 tcctgcgatc ggagcaacaa tcagggtgtt actcaggtca aacctggact tgaccaaatc
     2221 cccctgcgcc gcttccaggg cggctttggc cgctaacccc tccgccaggg catcgtccac
     2281 gtccttttga ctgaccgctt gttctcccag gagaggtttg acgcgagcaa aatcctgctt
     2341 cgcttggacc aatcgagcct ccgcttgagt caccctggct ttggcactga gatacgccgc
     2401 tttaaagggc acagggtcga tgaggtacag ccgatcacct tgcttgacat tcctgccttc
     2461 ggtgaaaaac accttcttaa tgatgcccgt tacctgggac cggatctcca ccgggcgaaa
     2521 ggcctcggtt tgtccgatga attccggttc atcggggaga tcctgggtag tcaccgtcac
     2581 cacttccacc atcggcggtg gaggagccgg gggtgaggtt gctccctgcc cacatcctgc
     2641 aagaaaaatc aggagtatcc cgatggtcca acgaatcacg acgccaccat tgaacaaggg
     2701 tgatgcgacc aacctgagta ttgctaaatt catatgccgg atccccacca actcccgcca
     2761 atcccattca gggcatcctt ggcaaaacgt tcacatagga cacttctctg aattatttga
     2821 tcatccatac ggttttactt ttcctatctt cagcatcagg agtgggattc ttttctcttt
     2881 gaaggtgtag acggaaaaga gccaccaatc ttacagttga aacatgagct ttacaatttc
     2941 ccccggactt atatgtattc ccaccatttc cgccccttcc atccatcccg actctctgtc
     3001 acttgaaaac cacattgccc gaacaaccgc ttaatcaaga tgtcccatcc ttcccgcacg
     3061 gtaatcgttc acggcttgca cgagttcttc ctccgtgttc atgacaaagg ggcccatccg
     3121 ggcgaccggt tccagaatcg gttccccact cagcaccaac acccttgcct cctccgtggc
     3181 ctcaagatcc accttcgtcc cctgcgggtc acagagggcc atttcggcct ccccgacttt
     3241 gtgtgatccg ttcaaaatca atcccccact caacacaaat aaaccggtat tatatccaac
     3301 cggaagattc agctcacccc gatgtccggc taccaatttc agatcataca ggtgcacggg
     3361 cgtgaacgtc tttgccggcc ctttgcgtcc ctcatattcc ccggcgatca cacggagcac
     3421 accgccttgt cccccaagat tggcttgagg aatgtcttca ttgagcaccg tttggtagcg
     3481 tggcgcgctc attttcaatg acctgggaag attgacccag agctgaatca tttctagcgt
     3541 gccgccctcc ctggcccagg cttgctcatg gagctcctca tggaccacac ccgacgccgc
     3601 cgtcatccat tgcacatcac ccggtccgat ggttcctccg tttccggctg aatcccgatg
     3661 cgcgactttt ccctgataga cgatggt
//
LOCUS       DATIAL010000103         6160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1415729, whole genome shotgun sequence.
ACCESSION   DATIAL010000103 DATIAL010000000
VERSION     DATIAL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415729"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VLA60_03405"
     CDS             <1..956
                     /locus_tag="VLA60_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSF08433.1"
                     /translation="TKQRLQHTNEELQTSNEELKSTNEELQSTNEELQSTNEELETSK
                     EELQSLNEELLTVNAELQGKIDEQSATNDDLHNLLNSTQIATIFLDNDLSIKRFTPEA
                     KQIVNLIATDVGRPLTDIASKLSDGRLIEHAQHVLRTLVPYHGEVQTTDGEWHVLRIL
                     PYRTARNTIDGLVLTFVNMTKVKKAEILARQAQEFAESIIQTVRQPLIVLDGQLRVVT
                     ANQVFFETFHLSPAEVEHRHFYEVGECEWDVPALRRLLEEILPQNSTFQDFLVDHTFA
                     RVGRRVFALNGRRLEQETGDSRLILLVMEDVTQKKPNLESR"
     gene            1162..3291
                     /locus_tag="VLA60_03410"
     CDS             1162..3291
                     /locus_tag="VLA60_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HSF08434.1"
                     /translation="MDPQKQAFTNSDTLRQKAEAGLTTTRAQVKNLTLEEVQRLVHEL
                     QVHQIELEMQNEALRATQMDLEIARDRYASLFDFTPVGNVTLDENGRILEANLTFGHL
                     VGMLRSAILQQKFEQFVDQEDQSAFRLHLESLKKSSGIRSSDVFTLRHSKTPHRVRLE
                     SSQEAGESPGRAKLIRIAVVDVTERERLAAVQEKHKALMEVVVGGVIDAIVTTEKDER
                     IVVFNKAAEKMLRCPASHAIGQPIDRFIPERFRKAHHMQHRPFGQDAVNNHHMDAARD
                     VILRRADGEEFSAGVTISKVEGKGWDKGKPLFILVLRDITDQCRQQDEQQRISKLNSL
                     GILAGGLAHNFNNLLTSILGNVFVAKLRMDPQDDHLNHNLEQAEQDCLRGKGLTQQLL
                     TFAKGGAPIKNSILVGDLLRKSTIFALSGSPISCDFNIPNDLWPLDADPGQFPQVIQN
                     ITINARQAMPEGGHLMVKVENVELKDRSVLPSPALVPGNYVKISFNDQGNGIENHHLS
                     KIFDPYYTTKPGALGLGLATAHSIIQQHHGHISVTSTVGIGTTFTVYVPSSQFTLEPG
                     STRVPVITKTNRGRVLVMDDEQSICRMVMDALTHFGYEVVTVGDGKAAIDQVSRSLAD
                     GSTFEVVILDLTIPGAMGGKEAIQHLRRIDPHIKAIVTSGYSEDPILSEFQKYGFQGI
                     LIKPYKISHLANLLETIGDPVPHDTIQ"
     gene            3854..4012
                     /locus_tag="VLA60_03415"
     CDS             3854..4012
                     /locus_tag="VLA60_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSF08435.1"
                     /translation="MIKSPCVYILASKPHGTLYIGVTSYLIKRCWEHQNGFVKGFTDT
                     YNVHRLVY"
     gene            4312..>6160
                     /locus_tag="VLA60_03420"
     CDS             4312..>6160
                     /locus_tag="VLA60_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08436.1"
                     /translation="MSLKKKPAGGLNVLRQQAEKALTTTRAQVKKMTPEEVQHLVHEL
                     QVHQIELEMQNEELRRTQVDLELARDRFATLYDFTPVGYATLDGGGNILEANLTFCHL
                     LGLSRTDILHKKFEKYLDPDDQPAFYRYLDTLRQKPGTHPSDVLTLRNSDLLHRVRLE
                     GCLEPTEASGIGSLFRIAVEDVTMQERIELAQEEQKALMTMVVGGVMEAIISTDEDER
                     IVLFNEAAETMLRCPASKAIGQTIDQFIPARFRAAHHTHHRQFGEEGVASRPMGVARE
                     IFALRTDGKEFPVEASISKIKLKEKEKKLFTVVLRDITERRQAQEMVKKEQQFASNVL
                     ETAGALLVILDPQWQILRMNRLSEEIFQHSIERVHGKPFWNLWASSEKEAQGVRQTVK
                     SYKAGTLPMSFESAVVDKDRQRRWIHWNTTAMYDEEKVVRQFIATGVDVTARKEAEIT
                     LQATYQRLERQQRELRSLAAQLLSVQEEERRRISRDLHDDVNQRLALLSLKLQAARDV
                     LPDHHLVTPMIQELYENVANLSDDIRHLAYQYHPSILDDLGLGSALRSLCEDFEKWER
                     ISVTCELPDGARKISQAVGTCLYRVAQESLRNVSKHTQASAVHLILREDE"
BASE COUNT         1613 a         1461 c         1649 g         1437 t
ORIGIN      
        1 ataccaaaca acggttacag cacacgaacg aagaattaca aacgtcaaat gaagaactga
       61 aatccaccaa tgaggaactt caatccacca atgaggaatt acaaagcacg aatgaagaat
      121 tagaaacgtc caaagaagaa ctgcaatcgt tgaatgagga attgctgacg gtcaatgccg
      181 aattgcaggg caagatcgac gaacagtctg cgaccaatga tgacttacac aacttactca
      241 acagcaccca aattgccacc atttttttgg acaacgatct cagcattaaa cggtttaccc
      301 cggaagccaa acagattgtc aaccttattg cgacggacgt cggccgacca ctgacggata
      361 tcgcctcgaa actttccgat ggtcgactta ttgagcatgc tcaacacgtt cttcggactc
      421 tcgtgcctta ccatggggag gttcaaacga ccgatgggga gtggcatgtt ctccggattc
      481 ttccctatcg gacggccaga aacacgattg atggtcttgt cctcaccttt gtgaatatga
      541 caaaagtgaa aaaagcggag atcctggcca ggcaggctca ggaatttgcc gagagtatta
      601 ttcagactgt ccgccaaccc ctgatcgttt tagatggaca gttacgagtc gtgactgcca
      661 atcaggtttt ttttgagacg tttcatctct cccctgcaga ggtcgagcac cgccattttt
      721 acgaggtggg agaatgtgaa tgggatgttc cagccttgcg tcgactgctc gaagaaattc
      781 tcccccaaaa cagcacgttt caagactttc tcgtcgatca cacctttgcc cgtgtcggac
      841 gccgtgtttt tgctctgaat ggccgtcgtc tggaacagga gacaggggat tcacggctca
      901 ttcttcttgt catggaggat gtgacgcaga aaaagccgaa tctggagtcc cgatgaaata
      961 tgacgggtat gacacattat ctttgtcatc ctgagtgaaa cgaaggatct cgctaagccg
     1021 tggtctcgtc aattttggta cagatccttc tcccctagct caggatgaca gacgctatgg
     1081 ttatccccac aattcattag cggggaggta tctttaggat cgtaccgcaa tacctattcc
     1141 tagtgccacg gagggcatca tatggatcca caaaaacagg cgttcaccaa ttccgacacc
     1201 ctacggcaaa aagctgaggc tgggctaacg accacgcgcg cgcaggtgaa gaatctgacg
     1261 ctcgaggagg tacagcgcct cgtgcatgaa ctccaagtac atcaaatcga actggaaatg
     1321 caaaacgagg cactacgagc aacccagatg gacctggaaa tcgcgcgtga tcgctatgcg
     1381 agtctctttg attttacacc ggtgggaaat gtgacactcg atgaaaatgg ccgcattctg
     1441 gaggccaatt tgacttttgg ccatttggtg ggaatgttac gaagcgcaat tctacaacaa
     1501 aaatttgaac aatttgtcga tcaggaagac caatcggcct ttcgccttca tttggagtcc
     1561 cttaaaaaga gctcagggat ccgctcgtcc gatgttttca ccctccggca ttcgaaaact
     1621 ccgcatcgag tccggttgga aagctcccag gaggcggggg agtctccggg ccgggcgaag
     1681 ctcattcgaa tcgctgttgt cgatgtgacc gagagggagc gcctggccgc tgtccaggaa
     1741 aagcacaaag ccttgatgga ggtggtggtg gggggagtga ttgatgcaat tgtgacgacc
     1801 gaaaaagatg agcggattgt ggtatttaac aaggctgccg agaaaatgtt gcgctgtccc
     1861 gcttcacatg cgattggaca gccaattgac cggtttattc cggaacggtt tcgaaaggcc
     1921 catcacatgc agcatcgacc atttggacag gatgcggtga acaatcatca catggatgct
     1981 gcacgagacg tcatactaag acgagcggac ggggaagaat tttcagctgg tgtgaccatt
     2041 tcgaaagttg agggaaaggg atgggataag ggaaagcccc tgtttattct ggtgcttcgg
     2101 gatatcaccg accaatgccg tcaacaagat gagcaacaac ggatcagcaa actcaattcg
     2161 ttaggtattc tggctggggg gctggcacac aatttcaata acttgctcac gtctattttg
     2221 gggaatgtgt ttgtggcgaa actgcgtatg gatccgcaag acgatcattt gaatcacaat
     2281 cttgagcaag cggaacagga ttgcctgcgt gggaaagggt tgacccaaca acttctgacc
     2341 tttgccaaag gcggggcccc gattaaaaat tccattttgg tcggagatct tctccgaaag
     2401 agtacgatct ttgccctctc cggatcgccg atcagttgtg attttaatat cccaaacgac
     2461 ctctggcccc tggatgccga ccccggccaa ttcccgcaag taattcaaaa catcacgatc
     2521 aatgccaggc aggctatgcc ggagggtggc cacctcatgg tcaaggtgga aaacgtggag
     2581 ttgaaggacc ggtcggttct tccttcaccg gcacttgttc ctgggaacta tgtcaaaatt
     2641 tcgtttaatg accaggggaa cggaattgag aaccatcacc ttagcaagat tttcgatccg
     2701 tattacacga caaagcctgg cgcgctcggg ttgggtttag ccactgccca tagcatcatc
     2761 caacagcatc atgggcatat tagtgtgaca tccaccgtgg ggatcggcac cacctttacc
     2821 gtgtatgtgc catcttccca attcacgctt gagcctggtt caacgagggt tccggtcatc
     2881 acgaaaacga atcgcggacg agtgctggtt atggatgacg aacagagtat ttgtcgaatg
     2941 gtgatggacg cactgaccca tttcggctac gaggtcgtga cggtggggga tgggaaagct
     3001 gccatcgacc aggtttcccg ttcactggcg gatgggagta cctttgaagt ggtgatcctt
     3061 gaccttacca tccccggcgc catggggggg aaagaggcca ttcagcatct tcgacgtatc
     3121 gatccccata tcaaggccat tgtcaccagc ggatattctg aagatcctat tttgagcgaa
     3181 tttcaaaaat atggattcca agggatccta atcaaaccct acaaaatttc ccatctcgcc
     3241 aatctactcg aaacgatagg cgatcccgtc cctcacgata cgattcagtg acgtctgagc
     3301 gacaattgcc tgttccgagc gtctaaggtc ttgatatcgg ttctgctaaa cggtgcgggc
     3361 cgaggctctc aacgaaggtg atggattctc gattccagat gtggtgaagg gccgtgagaa
     3421 tgatcccact tccaggggga caaagaacaa ggcctcaaag gccttgatat tccagcgagc
     3481 tgtacctttc ggttgaaaat gaatatgatt tgacttctgt gaaggtgcct gtccaccttg
     3541 ttcctaagga aaagtcttca tggatactcc agcaacgccc ataaaatcct ccggagtagg
     3601 gggatgcccg agagaaaatg tttggcatgg tctatggttt ccgtcatccc cgtcggtggt
     3661 aagcggggat ccagccggaa cgaatctcag atggactccc gatgaccaat gtcgggagta
     3721 acggcagggg atggatgccc gatgaacaat ctcgggcatg ccacctggga atagatgccc
     3781 gatacgagct gtcgagtttg tcacttttct tcgtcatccc cgcccgtggt gagcggggat
     3841 ccatctttca aatatgatta aatccccttg tgtgtatatt ttggccagta aacctcatgg
     3901 caccctatat attggagtaa ccagttatct catcaagcga tgctgggagc accaaaacgg
     3961 attcgtgaag ggatttaccg atacctacaa tgtccataga ttggtgtact aagaaattct
     4021 cccggacatg actttggcaa ttgccaggga aaagcaactt aagagatgga atcgcgcatg
     4081 gaagattcaa ttgattggaa aacagaattc cgaatggcgt gatctgtggg aagtgattgt
     4141 cttatgacgg gattcctggt tgcatagaca agtcaaggat aaagaccaaa tggatgtcgg
     4201 gcatgaccca tgtttccggc atccccgccg gttggtagcg gggatccatc cgaaggcaat
     4261 gccacctaaa ttcacgcctc tgcgggatga cttggggaaa gcaagaaacg catgagtcta
     4321 aagaaaaaac cagccggcgg gcttaacgtc ctacgtcaac aggctgagaa ggccctgacg
     4381 acaacgcgcg cacaggtgaa gaagatgacc cctgaggaag tgcagcacct tgtgcacgaa
     4441 ctccaagtgc atcaaatcga actggagatg caaaacgagg aacttaggcg gactcaggtt
     4501 gacctggagt tggctcgtga tcggtttgcc acgctgtatg atttcacacc agtgggatac
     4561 gctacactcg atggaggcgg gaacattctg gaagccaatt tgaccttctg ccatttgttg
     4621 ggcctgtcac gcacagatat tctccataag aaatttgaaa aatacctgga tccggatgac
     4681 caaccagcgt tttaccggta tttggatact ctgagacaga aaccaggcac tcacccttcc
     4741 gatgttctga ccctacggaa ttccgatttg ctgcaccgcg tccggttgga aggctgcctg
     4801 gagcccactg aagcttccgg tattggaagt ctgtttcgga tcgccgtcga ggatgtgaca
     4861 atgcaggaac gaatagaact cgcgcaggaa gagcaaaagg ccttgatgac gatggtagtg
     4921 ggcggggtga tggaggcgat catcagtact gatgaagatg agcggattgt gctgtttaac
     4981 gaggccgctg aaaccatgct tcgctgtccg gcttccaaag ccattgggca gacaattgac
     5041 caatttattc cggcccggtt tcgagcggcc catcacactc accatcgaca atttggagag
     5101 gagggggtgg cctcccgtcc aatgggtgtt gcaagggaaa tttttgcact tcgaactgac
     5161 ggaaaagaat tcccggttga agcctccatt tccaaaataa aactgaaaga aaaggaaaag
     5221 aaactgttta cagtggtgct gcgggacatt actgagcgtc gacaagccca ggagatggtg
     5281 aagaaagaac aacagtttgc ctctaatgtg ttggagacag caggagccct cctggttatt
     5341 ctggatccgc aatggcaaat tcttcgcatg aatcggttgt cggaagaaat atttcaacat
     5401 tccattgagc gtgtgcatgg gaagccgttt tggaatttgt gggcgagttc cgaaaaggag
     5461 gcacaagggg ttaggcaaac ggtgaagtct tacaaggctg gaacgttgcc tatgtcgttt
     5521 gagtcggccg tggtggataa agaccgtcag cgccgatgga ttcattggaa tacgaccgcc
     5581 atgtatgatg aggaaaaggt ggtgaggcaa ttcattgcga ccggagttga tgtgactgcg
     5641 agaaaggaag cggaaatcac tcttcaagcc acctatcaac ggctcgaacg gcaacagcgg
     5701 gagctgaggt cattggcggc ccaactgttg tcggtccagg aagaggaacg tcggcgtatt
     5761 tcccgtgatc tgcatgatga tgtcaatcaa cggcttgcgc ttctgagctt gaagctgcaa
     5821 gctgcgcgag acgtccttcc ggatcatcac ctcgtcacgc caatgatcca ggagctctat
     5881 gaaaacgtag ccaatctgtc cgatgatatc cggcatttag cttaccaata tcatccgtcg
     5941 atcctggatg atctgggatt gggctcggct cttcgttcat tgtgcgaaga ctttgagaag
     6001 tgggaacgga tctcggtcac ctgtgagttg cctgatggag cacgaaaaat ttctcaagcg
     6061 gtgggcacat gtttgtaccg agtggcacag gagagtttgc gaaatgtttc aaagcatact
     6121 caggcctcgg cagtccatct cattttgagg gaggacgagc
//
LOCUS       DATIAL010000104         3836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1415913, whole genome shotgun sequence.
ACCESSION   DATIAL010000104 DATIAL010000000
VERSION     DATIAL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415913"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VLA60_03425"
     CDS             complement(<1..189)
                     /locus_tag="VLA60_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08437.1"
                     /translation="MNAELITVGVQDNRCPAVRKVERLKGELHVVMSEMFYRLVEVVH
                     FQHKLRTVARWLQEWFFAD"
     gene            complement(457..888)
                     /locus_tag="VLA60_03430"
     CDS             complement(457..888)
                     /locus_tag="VLA60_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HSF08438.1"
                     /translation="MIAVDTNILVYAHREDSPWHNSAYDHLVSLAEGAKSWAIPWPCL
                     HEFLAIVTHPRIFKPPTPLQAACEQVDAWLESPKVLLLSETHDYWQVLRQAVIEGKVQ
                     GPLVHDARIAALCRHHGIQELWTADRDFSRFPTLSPRNPLL"
     gene            complement(885..1130)
                     /locus_tag="VLA60_03435"
     CDS             complement(885..1130)
                     /locus_tag="VLA60_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08439.1"
                     /translation="MKTTVDIPQSLLDEAKKLAANHRTTVKALIEEGLRRVISEHQRS
                     GVFRLRRATFKGEGLQPDMEGASWENLRERAYEGRGG"
     gene            complement(1281..2135)
                     /locus_tag="VLA60_03440"
     CDS             complement(1281..2135)
                     /locus_tag="VLA60_03440"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF08440.1"
                     /translation="MKTRLLVTGGAGFLGNHLLRRANQFVAAGTLHATPSTSLPGVTF
                     HVCNLQNPEEVRVLMGRVQPDVIIHTACSEQGKGIEAILPAAGLLAMQSRERNIRFIH
                     LSTDQVFDGTSAPYTEESPTNPINPYGKAKAQAEELIRSLNPQATIVRTSLLYDLRTP
                     DRQTTRLIESTKTGEPYRLFIDELRCPIWVENIAEVLLEVATKDVPGIVHLGGPESLN
                     RWDLGVGLLQHFGITQTSYIQQGTIEESGLVRPKNLTMDSSRTKQLLHTPILSLSEAR
                     EKVQHLKI"
     gene            2236..3276
                     /gene="ltaE"
                     /locus_tag="VLA60_03445"
     CDS             2236..3276
                     /gene="ltaE"
                     /locus_tag="VLA60_03445"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HSF08441.1"
                     /translation="MQKIDLRSDTVTKPTPAMREAMAQAEVGDDVYGEDPTVNRLEAM
                     AAEMLGKEAAVFVPSGVMGNQLALRLHTRPGDEVIVDSTSHLIRYEGGSASSLSGVQL
                     VCVPGDRGRLSPESVEAAIRPKGLHNPPTTLVCLEQTHNVGGGSIYSLELIHQIAEVA
                     RIHGLSLHLDGARLFNAVVSTGVAAADYARPFDTVSFCLSKGLGAPVGSMVVSDAARV
                     QTLRRLRKVFGGGMRQVGILAAAGVYALEHHIARLAEDHVNAHYLATLLEDIPGVVVD
                     VKAVETNMVMFQVPHSSKTTDTLLADCREAGVLLNAMGDRAFRVVTHLDVNHEDMVTA
                     GRVFRQVFSPAV"
     gene            3359..3592
                     /locus_tag="VLA60_03450"
     CDS             3359..3592
                     /locus_tag="VLA60_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08442.1"
                     /translation="MSLLDTVSFQHITRILEVTDELDISREAVEIPLAPASPGVVRRL
                     SNGKLEIVVDADLPFDDWVQTLPEKIQAEMPDD"
     gene            3645..>3836
                     /locus_tag="VLA60_03455"
     CDS             3645..>3836
                     /locus_tag="VLA60_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08443.1"
                     /translation="MAVRDREWPGYTIWYCCQECGRLWTYQGSDVVALDLCSSLGPAM
                     VGDGVRGRMCAICEGRCHAP"
BASE COUNT          821 a          876 c         1143 g          996 t
ORIGIN      
        1 atcggcaaag aaccattcct gaagccatcg ggcgacggtc ctcagcttgt gctggaagtg
       61 gacgacttcg acaagacgat agaacatctc agacatcaca acgtgcagtt cgcccttgag
      121 cctttcgacc ttcctcactg ccgggcagcg attgtcctgg accccgacgg taataagctc
      181 ggcattcata aacgaaaccc ctaacgaatt tggattaggg ttcaggtttg ttggtgagag
      241 tttaaaggta aggtatcagg agcatagctt ttgggtactc ttttccctgg ttttttccat
      301 gcagccgggg ttcctttggt ttggctcatg ggcgtaatca ggtcgtgccg gactattcat
      361 caggtttatc cctatgagaa cgaagtcatt tctcgttgtg atttttccat tcttcttaag
      421 caactccatt ccccatggag gacaggcgcc aatttattag aggagtgggt tgcgaggaga
      481 aagggtggga aatcgactga agtcacgatc cgctgtccac aactcctgaa tgccatgatg
      541 tcggcagaga gctgcgatgc gggcatcatg gacgagagga ccttgaactt ttccctctat
      601 gaccgcctgc cgtaatactt gccaatagtc atgtgtttct gaaaggagta agacttttgg
      661 ggattctaac caggcatcta cttgctcaca ggcagcctgg aggggtgtgg gtggtttgaa
      721 gattcgcgga tgagtcacaa tcgccagaaa ttcatgcagg catggccagg gaatggccca
      781 ggatttggcc ccttccgcca agcttactag atggtcgtag gccgagttat gccagggaga
      841 atcttcacga tgggcataca ctaagatatt cgtgtcaact gcaatcaccc tccgcgacct
      901 tcgtacgcgc gttcccggag gttttcccat gacgcccctt ccatgtctgg ctgaagacct
      961 tctcctttaa aggtcgccct gcgaaggcga aacacccctg aacgctgatg ctcacttatg
     1021 acacgccgta acccttcctc aatcaaggcc tttaccgtgg ttcgatgatt ggcggccagc
     1081 tttttagctt catctaaaag tgattgaggt atatcgacag ttgtcttcat atgtgtagat
     1141 gtatcatcta atatgggttt atgtcaaaac gaagaatttt aacggatagc cgtgggtaaa
     1201 tcccttgacg gcgaacatga ttgtaagtgt ttgggtataa gaagcttatg gctttttctt
     1261 cctggtggat gccagatttt ttagattttg agatgttgaa ctttttcccg ggcttctgaa
     1321 agggagagta tgggggtatg gagaagctgt ttggttcgag aagagtccat ggtgaggttt
     1381 ttggggcgaa ccaatccgga ttcctcgatg gtaccctgtt gaatatacga tgtttgtgtg
     1441 atcccaaagt gctggagcaa tcccactcct aagtcccacc ggttcaggct ttcagggccc
     1501 ccgagatgta caattcctgg cacatctttg gtggcgactt ccagtaacac ttcggcaata
     1561 ttttccaccc agatgggaca gcgaagttca tcaatgaaca agcgataggg ttcgccggtt
     1621 ttcgtggact cgattaagcg tgttgtctgg cggtccgggg tgcggagatc atagagcaag
     1681 gaggtgcgga cgatggtggc ctgtgggttc agggaacgga tgagttcttc cgcctgggct
     1741 ttggccttac cgtaggggtt gatggggttc gtggggctct cttcggtata gggcgcggaa
     1801 gtcccatcaa agacttgatc ggtcgagagg tgaatgaagc gaatgtttcg ttccctcgat
     1861 tgcatggcga ggagtcctgc tgcagggagg atcgcttcaa ttccttttcc ctgctccgag
     1921 caggcggtgt gaataatgac gtctggttgc acccgaccca tgaggactcg aacttcctca
     1981 gggttttgga gattacacac atgaaacgtc acgccgggga gggatgtgga gggcgtggcg
     2041 tgcagcgttc cggctgcgac aaactgattg gcccgacgta ggaggtgatt gcctaaaaag
     2101 cccgctccgc cggtgacgag gagacgtgtt ttcatcgccc gcattgtaga ccaatgccaa
     2161 aaggaaaatc ttgtgtgaag acagacggtt ccttagaata cctgcagggt caataatttt
     2221 ggaaggaatc ggttcatgca gaagattgat ttgcgcagtg ataccgtgac gaaaccgaca
     2281 cccgccatgc gggaggcgat ggctcaggcc gaggtgggcg atgatgtgta tggagaagat
     2341 ccgaccgtga atcgattgga agccatggcc gctgaaatgt taggcaagga ggccgcggtg
     2401 tttgttccct caggagtgat gggcaatcaa ttggctctcc ggttgcacac gcggcccggt
     2461 gatgaagtga tcgtggatag tacatcccat ctcattcgct atgaaggggg atcggcgtct
     2521 tcgctctccg gtgtccaatt ggtttgtgtg ccgggtgatc gcggtcgcct ttcgccggaa
     2581 tcggttgaag ccgctattcg gccaaaggga cttcataacc ctccaaccac gttggtgtgt
     2641 ctggagcaga cgcataatgt gggtggagga tcgatttatt ccttggagct catccatcaa
     2701 attgcagagg tggcccgcat ccatggactg tccctgcatt tggatggtgc tcgattgttc
     2761 aatgccgtgg tctcaaccgg tgtggcggct gctgactatg cccgtccctt tgatacggtg
     2821 tccttttgtt tgtcgaaagg tttgggggct cccgtcgggt cgatggtggt gtcggatgcg
     2881 gcacgcgttc aaacattgcg ccgtcttcgc aaagtgtttg gcggggggat gcggcaggtg
     2941 ggcattctgg ccgctgccgg ggtgtatgca ctggagcatc atattgcccg attggctgag
     3001 gatcatgtca atgcgcatta tctggcgaca ctcttagagg acatccccgg tgtggtcgtt
     3061 gatgtcaagg cagtggagac caatatggtg atgtttcagg ttccccattc ttctaaaacg
     3121 accgacacgt tgttagctga ttgccgggag gccggagtcc tgttgaatgc catgggggat
     3181 cgggcgtttc gggtggtgac gcatttggat gtcaatcatg aagatatggt tactgccggg
     3241 cgtgtcttca ggcaggtttt ttcacccgcg gtgtgaaagt cgccacccct gagttcgttg
     3301 acagggtctg ggacagttcc tacaatttct attgatcagc ctgatgaagg gagccaacgt
     3361 gagtttattg gatacggttt cgtttcagca tatcacgaga attttagaag tgacggatga
     3421 gttggatatt tcccgggagg cagtggaaat tcccctggcc ccggcaagtc caggagtggt
     3481 tcgtcgattg tccaatggca aactggaaat tgtcgtcgat gccgacctgc ccttcgatga
     3541 ttgggtacag acactgcctg aaaagattca ggcggaaatg ccggacgatt gagtccgatt
     3601 agtcagatcg tgtaaccaag atgtaggcaa aggagaccgt actcatggct gtgcgtgatc
     3661 gggagtggcc tgggtatacc atttggtatt gttgtcagga atgtggccgc ttgtggacat
     3721 atcaagggtc agatgtcgtg gcgctggacc tctgcagttc actgggacca gccatggtcg
     3781 gggacggcgt ccgcggacga atgtgtgcaa tttgtgaggg gcgatgtcac gccccg
//
LOCUS       DATIAL010000105         4963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_448423, whole genome shotgun sequence.
ACCESSION   DATIAL010000105 DATIAL010000000
VERSION     DATIAL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448423"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="nadA"
                     /locus_tag="VLA60_03460"
     CDS             complement(<1..456)
                     /gene="nadA"
                     /locus_tag="VLA60_03460"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSF08444.1"
                     /translation="MTLSTIIERPIADYQSLPETELFERIVEAKRRLGTDVLLLGHNY
                     QRDDVIVHADFRGDSLLLAKYAAEHPEHPYVVFCGVHFMAETADILSRSNQTVILPDM
                     AAGCSMADMASMEQVDDCWDRLGRFLPVEDTVTPIVYVNSAAALKAFCGE"
     regulatory      796..898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1002..2930
                     /gene="thiC"
                     /locus_tag="VLA60_03465"
     CDS             1002..2930
                     /gene="thiC"
                     /locus_tag="VLA60_03465"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HSF08445.1"
                     /translation="MTPAQTTSPSTPSLHVISEEPRIPLSSLSIQPFPGSQKIYRTGS
                     RPDVRVPMREIHQTSSRSVHDDTEVQNPPVIVYDTSGPYTDPGIQIDVRKGLHPIRKD
                     WIAERQDVEPLKEVSSRYGRIRANDPALQAIRFHLQRPPLRAKSGRNVTQLHYARKGI
                     VTPEMEFIAIRENQAREERLSAQHGTGQAFGVVQHPGQNWGASTPSYITPEFVRDEVA
                     RGRAIIPANINHPEIEPMIIGRNFLVKINANIGNSAVTSSIEEEVEKLIWSIRWGGDT
                     VMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVGGKPEELTWEIFRDTLVEQAE
                     QGVDYFTIHAGVRLAYVPLTASRMTGIVSRGGSIHAKWCLAHHQENFTYTHFEEICEI
                     MKSYDVSFSLGDGLRPGSLADANDAAQFAELETLGELTKIAWKHDVQVMIEGPGHVPM
                     QLIKENMDKQLAACDEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWYGCAMLCYVTP
                     KEHLGLPDKEDVKAGVMAYKIAAHAADLAKGHPGAQHRDNALSQARFEFRWQDQFNLS
                     LDPETAKDFHDATLPAQGAKLAHFCSMCGPHFCSMKITQDVREYAAEKQLADDAALKQ
                     GMQEKSEEFRKTGGDLYR"
     gene            3051..3851
                     /locus_tag="VLA60_03470"
     CDS             3051..3851
                     /locus_tag="VLA60_03470"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HSF08446.1"
                     /translation="MNPINIAPLRERDITRHIAREYYKEFDSLIESDIIIVGGGPSGL
                     LCARDLAAIGFRTLLIEQSLALGGGFWSGGFLMNKATICEPADRILEELGIPFKPIKD
                     CPGMTMVDPPHVTSRLISAAYEAGVKIMNLTKVVDLILRQDQRIEGVVVNNSTVEMAG
                     HDTIHVDPIALESQIVVDATGHDAVVVNLLHQRNLYQKVPGNGAMWVARSEALVVENT
                     REIYPNCFVTGLAVAAVDGSPRMGPAFGSMLLSGRRAAELVQHKLKGE"
     gene            3904..4419
                     /locus_tag="VLA60_03475"
     CDS             3904..4419
                     /locus_tag="VLA60_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF08447.1"
                     /translation="MDIQDFLDQGEGNEEDKVAAWNLFQQAYELQMKGQLEEAVDLYK
                     QSIDLFPTAEAHTFLGWAYSFMGQLQEAIEECHQAIRQDPEFGNPYNDIGAYLIELNQ
                     LEEAIPWLEKAMKAKRYENPSFPHMNLGRVYERQGAWDQAVDSYKKSLALNPEYKTAK
                     QALMRILTLMN"
     gene            4438..4821
                     /locus_tag="VLA60_03480"
     CDS             4438..4821
                     /locus_tag="VLA60_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF08448.1"
                     /translation="MPDTKTILVAVSDIFFYTKIRDAFLPQGYTLERLRTGDDWQTKA
                     LASQPMGIIINMNDDRLNASEIVKSLRTLPQTHSLPILAFANHEEVQTWKLAKDLGIQ
                     KIVSRNEFSARTLALFEEITAATTS"
BASE COUNT         1350 a         1307 c         1184 g         1122 t
ORIGIN      
        1 ttccccgcaa aaggctttga gcgcagcagc cgaattcaca tagacgattg gggttacagt
       61 gtcttctacc ggcaaaaacc gtccgagccg atcccagcaa tcatccacct gttccatgga
      121 tgccatatcc gccatagaac agcctgcagc catgtcaggc agaatgacgg tttgattgga
      181 ccgactcaat atgtcggctg tttcagccat aaaatgcacc ccacaaaaca ccacataagg
      241 atgctctggg tgctcagctg catacttggc aagaagaaga gaatcgcctc ggaaatcggc
      301 atgcacaatc acgtcgtccc gctgataatt gtgcccgagc aggagcacgt ccgtgccaag
      361 tctccgctta gcctcgacaa ttcgttcgaa caattccgtc tctgggagag attgatagtc
      421 cgcaatgggc cgctctatga tggtcgataa ggtcacgcca aattccttaa gttaatgagt
      481 aaattctttc agatcagtcc acagcttcgc attgatcatc ttgatcatgt ctcaattgca
      541 tgggaaacca gtctttcatt gtagcaaatc attccctcat gaaaaagcct gaatgcctga
      601 gagcgaaatc cccttggcct acgtataaga tgatcgattc aatttctgca cacaacaacg
      661 ttaccgtcaa acctttcggt gacacacttt gaaatcaaag tgatagcgct ttctttacat
      721 gaacattttc agcgattgat ccaccatctc gcatggcgta agggtttgac aagggaaaag
      781 acccagcgtt acgatgattt tcgctagggg tgcgacacgc tgagagtccc aataaggggg
      841 acgaccctca cgacctgacc tggataatac cagcgtaggg aagcaggagc cacgaaagcc
      901 cgcacaagca gccgcactct ttagctgaaa aggggcggct ttttgttttt tggaataagc
      961 tccctggacc aattccctaa ccccagaaag agagggtttg catgacacca gcccaaacca
     1021 ccagtccttc cacaccatca ttacacgtca tttcggagga gccccgcatt cctctttctt
     1081 ccttatcgat tcaaccgttt cccggctcac aaaaaatata tcgaaccggc tcgagaccgg
     1141 atgtacgggt ccctatgcga gaaattcatc aaacaagcag tcggagcgtg cacgatgaca
     1201 ccgaagttca gaacccccct gtcattgtct atgatacctc aggaccctac acggatcctg
     1261 ggattcagat tgatgtgcgc aaggggcttc atcccattag aaaggattgg atcgccgaac
     1321 gtcaggacgt cgaacccttg aaagaagtca gctcacgata tggacgaatt cgtgcaaacg
     1381 atcccgcttt acaggccatc cggttccatt tgcaacgccc gccccttcgg gcgaaatcag
     1441 gccggaacgt cacacaattg cattatgccc ggaagggaat cgtcacaccg gaaatggaat
     1501 tcattgcaat ccgggagaac caagcacgtg aagagcgcct atccgcccaa cacgggacag
     1561 gacaggcctt cggggtagtt caacatcctg gacaaaattg gggcgcgtct actccctcct
     1621 acatcacacc ggagtttgtc cgtgatgaag tggctcgagg acgggccatt attcctgcca
     1681 atatcaatca tccagaaatt gagcctatga ttatcgggcg caattttttg gtaaaaatca
     1741 acgccaacat tggaaattct gccgttacgt cctctattga agaagaagtt gaaaaattaa
     1801 tctggtccat ccgatgggga ggagacaccg tcatggacct ctccacaggg aaaaacatcc
     1861 acgagacccg tgaatggatc atacgaaatt ctcccgtgcc catagggacc gtacccatct
     1921 accaggcctt agaaaaagtg ggcgggaaac cagaggaact gacctgggaa atctttcgag
     1981 atacactcgt agaacaggcc gagcaaggcg tggattattt caccattcat gccggtgttc
     2041 gcctggccta tgtcccactg acggcctctc ggatgaccgg catcgtctca cgcggcggat
     2101 ccatccatgc caaatggtgc ctggctcacc atcaggaaaa ctttacctat acgcattttg
     2161 aagaaatttg cgagatcatg aaaagttatg atgtgtcatt tagcttggga gacggcttgc
     2221 gaccgggttc actggcggac gccaacgatg cggctcaatt tgccgaactg gaaacgctgg
     2281 gagaactcac gaaaatcgcc tggaagcatg acgtccaagt catgattgag ggcccgggac
     2341 acgtccccat gcaactcatc aaagaaaaca tggacaaaca attggctgcc tgtgacgaag
     2401 ccccatttta cactctgggc ccgcttacca ctgatattgc gccaggctac gaccacatca
     2461 catccgccat cggcgcagcc atgatcggct ggtacgggtg cgcgatgctg tgttacgtca
     2521 cccccaaaga acatctaggg cttcctgaca aagaagatgt aaaagccgga gtgatggcgt
     2581 acaaaattgc ggcacatgca gcagatttgg ctaaaggcca cccgggggct caacaccggg
     2641 acaatgccct ctcacaggca cggtttgaat ttcgatggca ggatcaattc aatttatcgc
     2701 ttgatccgga aacggcgaag gatttccatg atgccacact ccccgctcag ggggctaaac
     2761 tggcccattt ttgctccatg tgtggcccgc atttttgttc tatgaaaatc acccaggacg
     2821 tgcgagagta tgctgcggaa aagcaattgg cagatgatgc cgccctgaaa caaggcatgc
     2881 aggaaaaatc agaagagttc cgaaaaaccg gtggagactt atatcgataa agcaggaagg
     2941 gtcaatgaca gaacctggag aaaaccagca ggaatttgta cagagacaaa gaccaaaacg
     3001 tgattggcct ttatgttgga taaaaacgaa atattacctg gagctcccct atgaatccta
     3061 ttaacatcgc tccgttacga gaacgggaca tcacccgtca cattgcccgg gagtattaca
     3121 aagaattcga ttcactgatt gaaagcgata tcattattgt cggagggggt ccatccggac
     3181 tcctttgtgc ccgcgatctc gccgcgatcg gcttccggac tctgctcatc gaacaatcac
     3241 tggctcttgg tggcggcttt tggtctgggg gatttctcat gaataaggcc acgatttgcg
     3301 aaccggcgga tcggattctt gaggaattgg gcataccctt taaaccgatt aaagactgcc
     3361 ctggcatgac catggttgat cctcctcatg tgaccagccg gctcatttcc gccgcttacg
     3421 aagccggcgt gaagattatg aatctcacca aagtcgtcga tctcattctt cggcaagacc
     3481 aacgaatcga gggtgtcgtt gtcaataatt ccactgtgga aatggccgga catgatacta
     3541 ttcacgtaga tcccattgcc cttgaaagtc aaattgtggt tgatgctacc ggtcatgatg
     3601 ccgtcgtggt taatctcctc caccaacgta acttatatca gaaggttcct ggaaacggag
     3661 ccatgtgggt agcgcgttct gaagcattgg tggtagagaa tacccgtgaa atctatccaa
     3721 attgctttgt gaccggctta gccgttgctg cagttgatgg cagtccacgc atgggaccgg
     3781 cgtttgggtc gatgttgctt tccggccgac gtgcagctga attagttcaa cacaagctca
     3841 aaggcgagta accgcctcta ttcctgatag acacaaaaag gactgtgcgc aatacaattt
     3901 cctatggata ttcaagattt tctcgaccag ggtgagggca acgaagagga taaggtggca
     3961 gcctggaacc ttttccagca ggcctatgag ctccaaatga agggacagct ggaagaagcg
     4021 gtggatctct acaaacaatc catcgacctc tttccgaccg ccgaagcaca cacatttcta
     4081 ggatgggcct atagtttcat gggacaattg caggaagcca ttgaggaatg ccatcaggct
     4141 attcggcagg accctgaatt cggaaatccc tataacgaca tcggggccta ccttattgag
     4201 ctcaatcagc tggaagaggc cattccctgg cttgaaaaag cgatgaaagc caaacgatat
     4261 gaaaacccct cctttcctca tatgaatctc ggacgggtat acgaacgaca aggggcgtgg
     4321 gaccaagccg tcgattccta caaaaagtct ctggctctta accccgaata caagacagct
     4381 aaacaagcgc tcatgcgcat tctgactctc atgaactaac tgtaaaggcc ttaccaaatg
     4441 ccagacacca aaacaattct tgtggccgtg agtgatatct tcttttacac caaaatacgc
     4501 gatgcctttt taccacaagg ctacacactg gaacgcctcc gtaccggaga tgactggcaa
     4561 acaaaagctc ttgccagcca acccatgggc atcattatta acatgaacga tgaccgtctt
     4621 aatgcctccg aaattgtgaa atctctacga acccttcctc aaacacactc attgcccatt
     4681 ctggcctttg ccaatcatga agaggttcaa acctggaaac tggccaaaga ccttggaatt
     4741 caaaaaattg tctcgcgcaa tgagttttcg gcgcggacac tcgcgctgtt tgaggaaatc
     4801 acggcggcca ctacatcatg aaaacactac ctcttcataa gcagcatgaa gccctaggag
     4861 ccacgtttca accttgtggg gaatgggaag tcccattgca ttacggtaat gctgcctcag
     4921 agtatgagtc cattcatcgg cgagccggcc tcgccgacct ctc
//
LOCUS       DATIAL010000106         4439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_18600, whole genome shotgun sequence.
ACCESSION   DATIAL010000106 DATIAL010000000
VERSION     DATIAL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18600"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VLA60_03485"
     CDS             complement(<1..970)
                     /locus_tag="VLA60_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF08449.1"
                     /translation="MDKQRDIPEVLSTPTWEIPIEDSHDLQLDAPSRSAGGIPAILTS
                     LKHTAHELGFLRGGQALLKVNQKQGFDCPGCGWPDPDGGRAITEFCENGVKAVAHEAT
                     TNIITKEFFQRYSLEQLSRQSDYWLGQQGRLIRPMIKRREDTHYHPISWVKAFETIAG
                     HLNNLDDPNEAIFYTSGRTSNEAAFLYQLFARLYGTNNLPDSSNMCHESSSVALKEVI
                     GIGKATITLHDFDKADAIFILGQNPGTNHPRMLATLQQAARRGCQIVSINPLPEAGTT
                     RFIHPREVTRWLGKGTPIATLFLPIKINGDVALLKGIMKELLMREEK"
     gene            complement(885..1862)
                     /gene="fdhD"
                     /locus_tag="VLA60_03490"
     CDS             complement(885..1862)
                     /gene="fdhD"
                     /locus_tag="VLA60_03490"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HSF08450.1"
                     /translation="MATKLYSTQDTTFFAGSARPLAHARADQLATEEPLEIRLEYWEH
                     NQPHRKSVSITMRTPGQDMELAAGFLFTEGIIAGWNDIQQIRPCGPIVEGQNFHNIVR
                     VKLHSHVSVKTSTMDRNFYTTSSCGICGKTSIEALKINNQFGQTIKNLTSPSVTPDML
                     FCLPERMAAEQELFQKTGGCHASGLFDEKGTLLCVREDVGRHNALDKVLGWALINNHL
                     PLHHHVVLVSGRASFELMQKSSMGGIPFVAAVGAPSTLAVQMAQEFNMTLVGFLDHQR
                     MTVYHDSGRIQASQAQQLEDTGGQTTRHTGSPVNPDVGNPDRRFSRPST"
     gene            2207..3640
                     /locus_tag="VLA60_03495"
     CDS             2207..3640
                     /locus_tag="VLA60_03495"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF08451.1"
                     /translation="MKILLTGATGYVGGRLLARLEQHGRKVRCLARRPEFLKEGVGPD
                     TEVVAGDVLDPASLATAMSGVEVAYYLVHSMGASGGFEETDRIAARNFGKAAWSAGLK
                     RLIYLGGLGDDSESLSPHLRSRHEVGEILRQSGVPVVELRASIVIGSGSLSFEMIRAL
                     VERLPVMLTPRWVHVPAQPIAIDDVLAYLIASLDVPLLESLIVEIGGDDIVSYGDLMR
                     EYAHQRGMRRIMIPVPVLTPRLSSLWLGLVTPLYACVGRKLIQSIRYPTVVRDHSARR
                     LYAIEPISVREAIAHALRNEDRELAESRWSDALSASGDVRQYGGVRFGNRLHDVRTVQ
                     VPVSNPDAFRPIQRIGGSTGWYFADWLWALRGWLDLLVGGVGLRRGRRDPEALRVGDV
                     VDWWRVEAIEPGRRLRLFAEMKLPGRAWLEYLVEPEGAGSRITQTASFDPIGLGGLVY
                     WYGIWPVHELVFRGMIRGIAREAQRSK"
     gene            complement(3785..>4439)
                     /locus_tag="VLA60_03500"
     CDS             complement(3785..>4439)
                     /locus_tag="VLA60_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08452.1"
                     /translation="VLLNNPLRTTSDYWRLVRNDEAVPREIGNHDYFYQIKYMAGRMD
                     LLPSMRLLKIATRSGKEKLNPVVTPEGSYAVNSEGFRLTTRIFEEFYCAALQHESLPV
                     IIIFPDLGDFSRHRSHQGKRYEPLLEELHIKGFRVLDILDALVAFDSALPTDRFTVGK
                     WGHFSRLGNSIVATFLQNYLNNEKLTVRDNVKNLVRAACTTNHCCPNSAPRKFTTAD"
BASE COUNT          880 a         1062 c         1346 g         1151 t
ORIGIN      
        1 ttttttcttc ccgcatgagc agttccttca tgatgccttt gagcagggcc acgtcgccgt
       61 tgattttgat tgggagaaac agcgtggcga tcggagttcc cttgcccagc cagcgggtga
      121 cttcccgtgg atgaatgaac cgggtggtcc cggcttcggg aagtggatta atgctgacaa
      181 tctgacagcc tcgccgtgcg gcctgctgaa gggtggcgag catacgagga tggttggtgc
      241 ccggattttg ccccagaatg aagatggcgt cggccttgtc gaaatcgtgg agcgtgatag
      301 tggctttgcc gatgccgatg acttctttta acgcgacgct gctggattcg tggcacatgt
      361 tggaggaatc gggcagattg ttggtgccat agagtcgcgc gaagagttga tagaggaagg
      421 cggcttcgtt gctggtgcgc ccggatgtat agaagatggc ttcgttggga tcgtcgaggt
      481 tattcaggtg ccccgcgatg gtttcaaatg ctttgaccca tgaaatggga tgataatgcg
      541 tatcctccct ccgcttgatc atcggacgga taaggcgtcc ctgctgccct agccagtaat
      601 ctgattgtct gctcagttgt tccagggaat atcgttggaa gaattctttc gtgatgatgt
      661 tggtggtggc ttcgtgggcg acggctttga ccccgttttc acaaaactcc gtgatggccc
      721 gtcccccatc gggatcaggc cagccacacc cggggcaatc aaacccttgt ttttgattga
      781 ccttaaggag ggcctggcca cctcgcagaa agccgagttc gtgcgcggtg tgtttgaggg
      841 aagtgagaat ggcggggatg cccccggcgc tcctggaggg cgcgtcaagt tgaaggtcgt
      901 gagaatcttc gatcgggatt tcccacgtcg gggttgacag gacttccggt atgtcgcgtt
      961 gtttgtccac ctgtgtcctc tagttgttga gcctgggagg cttgaattcg tccggagtca
     1021 tgatagaccg tcatgcgttg atggtctaaa aaccccacca gggtcatgtt gaattcttgt
     1081 gccatctgca ccgcgagtgt ggagggtgcg cccacggcgg caacaaacgg gatgccgccc
     1141 atggaggatt tctgcatgag ttcgaagctg gcccgaccac tgaccagcac cacatggtga
     1201 tgcaggggaa ggtggttatt gatcagggcc caccccaaga ctttatccag cgcattgtgt
     1261 cgcccgacat cttcccgcac acagagcaga gtgcccttct catcaaacag gcctgaggca
     1321 tgacatcccc cggttttctg aaagagttct tgctcggcgg ccatgcgttc cggcagacag
     1381 aataacatgt ccggggtgac ggaaggggag gtgagatttt ttatagtttg gccgaattgg
     1441 ttgttgattt tgagggcctc gatggaggtt ttgccgcaga tcccgcaact ggaggtggta
     1501 taaaaattcc ggtccatcgt tgaggtcttc accgacacgt gagagtgcag cttcactcgc
     1561 acaatattgt gaaagttctg cccttcgacg attggtccac atgggcgtat ttgttgaatg
     1621 tcgttccatc cggcaatgat cccttcggta aaaagaaatc ccgcagccag ttccatatcc
     1681 tgtccagggg tccgcatggt tatcgaaaca cttttccgat gaggctggtt gtgttcccaa
     1741 tactccagtc gaatttcaag cggttcttca gtcgctaact ggtctgctct ggcgtgtgca
     1801 agcgggcggg cggaaccggc gaaaaaggtc gtgtcctgcg tcgaatagag tttggttgcc
     1861 atgctcacaa cctttttttt tatggttgaa tgcctaaccc ccagtggcat cactgggata
     1921 ttgttattat agcccccttc cttcttctat gcacggcaga tttcttcgta gtcgaagaag
     1981 atctgatgtg tagaatccca ggcctcgctt gcattgtctt ccctaccatc gacaacgtca
     2041 tcctgagaaa atcaaagggc ctcgccaggc catgaggcac ctcgtcttcg gtaacccttc
     2101 atctcaattt gaggtctttt ttggctatat tgtgtgggtg ctttaccctt cttctctttg
     2161 gacggcacat ttcctgatag gatagtgaat gctcgaaaga tgacacatga aaattcttct
     2221 gacaggagcg acagggtatg tgggcgggag actgctagct cggctggagc agcatggcag
     2281 gaaggtacgg tgccttgcgc gacggcccga gtttttgaaa gagggggtcg gtcccgacac
     2341 ggaggttgtt gccggtgatg tgcttgaccc ggccagcttg gcgaccgcta tgagtggagt
     2401 ggaagtggcc tattaccttg tgcattccat gggggcgtcc ggaggtttcg aggaaactga
     2461 ccgtatcgca gcgcggaatt tcggcaaagc cgcttggtcc gccggcctga aacgccttat
     2521 ttatcttggg gggcttgggg acgacagtga atcattgtct cctcatctgc ggagccggca
     2581 tgaagtaggg gaaatccttc gtcaatccgg tgtgccggtc gtggaattgc gggcgtcgat
     2641 cgtgatcggc tctggcagtt tgtcatttga aatgattcgc gctctggttg agcggttgcc
     2701 ggtgatgctg acgcctcgct gggttcatgt ccccgcccaa cccatcgcga tcgatgatgt
     2761 gctggcctat ctgattgcct ctcttgatgt gccacttctg gaaagcctga ttgtggaaat
     2821 tggtggggac gatattgtgt cgtatgggga tttgatgcgg gagtatgctc accagcgtgg
     2881 aatgcggcgg atcatgattc cggtgcctgt tctcacgccg cgactgtcga gcctatggct
     2941 tggccttgtg acgccgttgt atgcgtgtgt cgggcgtaag ctcatccaaa gcatcaggta
     3001 tcctaccgtg gttcgggacc attctgcgag acgtttgtat gcaatcgaac ccatcagtgt
     3061 gcgggaggcc attgcgcacg cgttacgcaa tgaagaccgg gaacttgcgg aatcgcgttg
     3121 gtcggatgcg ctttctgcgt caggggacgt gcgacagtat gggggggtgc gattcggcaa
     3181 tcggctgcat gatgtgcgca cggtgcaggt acctgtctcc aaccctgacg cgttccgccc
     3241 gattcaacgg atcggcggga gtaccggctg gtactttgcc gattggctgt gggcgctccg
     3301 gggctggttg gatttattgg taggaggggt aggccttcgt cgtggccggc gggatccgga
     3361 agccttgcgg gtgggggacg tggtggattg gtggcgggtg gaggctatcg agccggggag
     3421 gcgtctccgg ctctttgccg aaatgaagtt gcccggacgc gcctggctcg agtatctggt
     3481 ggaacctgag ggtgccggct cgagaattac ccagacagcc agcttcgatc ccattggtct
     3541 gggaggtttg gtgtattggt atgggatttg gccggtgcat gaactggtct ttcgcggaat
     3601 gataagaggt atcgcccggg aagcgcagcg atcgaaatga ctttcaccaa tgttttgcat
     3661 gcctcagtct gcaagccgtt ttgagttttt cttctccttc ggctagaaag gtcacatctg
     3721 caaaacataa atccgctttt ggcggacaag tcatgatgag actggtaggt tattcagaat
     3781 ctggtcaatc ggctgtggtg aattttcggg gagcggagtt cggacagcaa tggtttgtgg
     3841 tgcaggctgc gcgaacgaga ttctttacgt tatcccttac cgtcaatttc tcgttgttca
     3901 aataattttg gaggaaggtg gcgacaattg aattcccgag gcgggagaag tgtccccact
     3961 ttccaaccgt gaatcgatct gtgggcagcg cagagtcaaa ggcaaccaat gcatctaata
     4021 tgtctagaac acgaaatcct tttatgtgta attcctccaa cagtggttca taccgtttcc
     4081 cctggtggct gcgatgccta ctgaaatcgc ccaggtcagg aaagatgata atgacaggta
     4141 aggattcatg ctggagtgcc gcacaataga actcctcaaa tatccttgtg gtgaggcgga
     4201 agccttctga attgacggca taagaccctt cgggtgtcac caccggattg agcttttcct
     4261 tcccactccg tgtggcaatc tttaggaggc gcatggaggg caggagatcc attcgtcccg
     4321 ccatatattt tatctgataa aaataatcat ggttcccgat ttctcggggg acggcctcat
     4381 cgtttctgac tagacgccaa taatcgctgg tggtccttaa gggattgttg aggagtaca
//
LOCUS       DATIAL010000107         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1309087, whole genome shotgun sequence.
ACCESSION   DATIAL010000107 DATIAL010000000
VERSION     DATIAL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309087"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..550)
                     /locus_tag="VLA60_03505"
     CDS             complement(317..550)
                     /locus_tag="VLA60_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSF08453.1"
                     /translation="MPRATSRAPSIPKDRWHDPKDLEAKLQKNKVNVLYCYSHVCHLA
                     ASVAVEMSGKGYPVMELEGGWRWWKNDGFDIEK"
     gene            856..>2228
                     /locus_tag="VLA60_03510"
     CDS             856..>2228
                     /locus_tag="VLA60_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HSF08454.1"
                     /translation="MSTWKRGIALLPSLFVAVACSHTIPPYTAKPVPPQQFTPPTPGA
                     IEVPVVQLPDSALPVTLVLDLTSLEQTLQASMPEHFSEAFHPLKNDYRWDFVREAEPQ
                     VSIQDGFVTIHSTYRGDIEAKTELRGCRLDPIFPILHTTGQLELGQVGDALVLSLKSP
                     QMDIDLKPESESKCNMFNIPVKDQLAELLNTPVLVENMTRAVDEGGFQIPLEQVWTRL
                     QAPVAVNVVKFNTQACIYGKPTEMAIGTLKGNVQRTTIPIVVNETPTARFENSCGKPS
                     AEAMKITSGASLLEGKPYKVLASVSVPYSEVNRGLQERLVHQPMKDGNVAMVIDKATA
                     SDSSGKVLLTINTTGDLNGTIYYWGTPRLEGEGSVLTMSDLQMASESKTMLDDIKVGY
                     WQIVDQQLRDKLQTATQVDLSDRIAKMKSAITGTHASGDVTREMTIAQQQPQRAYSIP
                     GALVADIL"
BASE COUNT          582 a          588 c          563 g          495 t
ORIGIN      
        1 gaagagagga gtaataagaa tagcaaaccg gcaatatttg cgaaaccaca cgcaaacgat
       61 acgcaagagt tggagttgaa agggtaagga cttgataccg gaaccgggaa cagcctgagg
      121 aaaaggatta ctgcgtcaca accacatgaa agaggtcacg cgaggcagga caaactattc
      181 tgtttccttt attggctacc aactatcttc ctaattcctc ttgggtgcga ggttgaaggt
      241 acttggctat aagtattcaa attacccaag cctataaccg gtgttaacac cttttctatc
      301 tcatggaagc ggattgtcac ttttcaatat caaatccgtc gtttttccac cagcgccatc
      361 cgccctccaa ctccatcacc ggataccctt tcccggacat ctccaccgca actgaggcag
      421 caagatgaca gacatgggaa tagcagtaca ggacgttgac tttgtttttc tgtagcttcg
      481 cttcaaggtc tttgggatca tgccaccgat cctttggaat tgatggcgct cgggatgtgg
      541 ccctcggcat aatcctcggc gcctgcctcc agtgttctcc ctgggttgaa tatttcttcc
      601 ccacatgcat gtattttatc catcgccttt actttattga tcctgatatt gatgtgctga
      661 aggaaaagga gaggaggaaa aagttgtcgg acagccagaa ctgctccagg caacagtaaa
      721 aacacaatcc aacaacaaag gagatgacgg ccgatagcca cgtccgtttg gcactagggc
      781 agtgggcgtc gaagtgtttc ggcagaaatt tcttccattt tatgtgaacg ggttgtgata
      841 cccaaggaga atactatgag cacctggaaa cgaggcattg cattattacc ctctctgttc
      901 gtcgcggtcg cgtgcagcca tacgataccg ccgtacacgg ccaaaccagt tccgccccag
      961 caatttaccc caccgacccc cggcgcgatt gaggtgcccg tggtccaact tcctgactca
     1021 gcgcttcctg ttacgctggt ccttgatctg acttccttgg aacagacatt acaggcatct
     1081 atgccggaac actttagcga agcctttcat ccgttgaaga atgactacag atgggatttt
     1141 gtcagagaag cagagccaca agtctccatt caggatggtt ttgtgaccat ccattccaca
     1201 tacagaggag atattgaagc caaaactgaa ctccggggct gtcgtttaga tcccatcttt
     1261 cccatcctcc ataccacagg gcaacttgag ttgggacagg tgggtgatgc gctcgtcctg
     1321 agtctcaaaa gccctcaaat ggacatcgac ctcaaacctg agagcgagag taagtgcaac
     1381 atgtttaata tcccggtcaa agatcagctg gcggagctcc tgaacacccc cgtgctggtc
     1441 gagaacatga ctcgggccgt cgatgaaggc ggattccaga ttcccctgga acaggtctgg
     1501 acgcgattac aagcgccggt ggccgtgaat gtggtgaagt ttaataccca ggcctgcatc
     1561 tatggaaaac ccacagagat ggcgatcgga accctcaagg gcaacgtgca gcggacgacc
     1621 attcctatcg tcgtcaacga aaccccgacg gcgcgttttg aaaattcgtg cggcaagcca
     1681 tccgccgaag cgatgaaaat cacctccgga gcctctctcc ttgagggaaa gccgtataaa
     1741 gtcctggcat ccgtgagcgt gccatactcc gaggtgaacc gagggctgca ggagagactg
     1801 gtgcaccaac ccatgaaaga cgggaacgtc gccatggtta tcgacaaggc cacggcttct
     1861 gactcgtcgg ggaaagtgct gctcaccatc aacaccactg gagatctcaa tggcacgatc
     1921 tattattggg gaacgccgcg tctggaaggg gaggggtctg tgctgacgat gtctgacctt
     1981 cagatggcca gcgagtcgaa aacaatgttg gatgatatca aggtgggcta ttggcagatc
     2041 gttgaccaac aattgcgaga caaattacaa accgcaacac aggtcgatct gtccgaccga
     2101 atcgcaaaga tgaaatctgc gatcacaggc acacatgcca gcggcgacgt aaccagggaa
     2161 atgacgatcg cccagcagca accgcaacgc gcctattcca tccctggggc gctcgtggct
     2221 gatatcct
//
LOCUS       DATIAL010000108         5150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1201419, whole genome shotgun sequence.
ACCESSION   DATIAL010000108 DATIAL010000000
VERSION     DATIAL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201419"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            727..1461
                     /locus_tag="VLA60_03515"
     CDS             727..1461
                     /locus_tag="VLA60_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HSF08455.1"
                     /translation="MSTISTHTVRSSIFAKYQPIIDLSTNRIVGCEALARWRADGGRE
                     ISIEPVIEEFESQEDLALELTTCMFACLKADLGTLLLEHPTFSVSVNLPPIVMGKNKI
                     LPVLEQLGLIPHLQQLTGEITERQALNEKGRDAIRLARQLGARVAIDDFGTGHSGLQQ
                     LIDLEVDTLKIDQSFIQQLGTNVAAERLVRGIAALATILEVDIVAEGIETPEQADFLR
                     AIGIEKGQGWLWAKALDANELYAWLD"
     gene            complement(1843..2220)
                     /locus_tag="VLA60_03520"
     CDS             complement(1843..2220)
                     /locus_tag="VLA60_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08456.1"
                     /translation="MRQVVAGWLGKQGVIIREEQEDATYRVHLIVESIGTTQNARLLG
                     LPATQATIFPIATPELAFYKRVQSQGYVRFYFDIFERASGRFVLSTEPQIGSVTQTVY
                     TAFFFFNWRKTDMEASPPIFENN"
     gene            complement(2277..2462)
                     /locus_tag="VLA60_03525"
     CDS             complement(2277..2462)
                     /locus_tag="VLA60_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08457.1"
                     /translation="MTRLKTVRAIMIYWLILSGCAIPSKKATDPLTPTEQLLFSSAIN
                     KDLKNYDVDILKEHQYS"
     gene            complement(2668..3321)
                     /locus_tag="VLA60_03530"
     CDS             complement(2668..3321)
                     /locus_tag="VLA60_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08458.1"
                     /translation="MSPSKYLVVMVTCLIMITMGLSPEVAAKSPKSEPQTSQWAWLGE
                     NGGTYGYVPSEYLKAYTWDTDDPSNPNTTVPIDDQTVWHIEKFDDGFFFGPVVVKFEG
                     QPALCQYMIGSVTPGPPSTPGQPGGRVEISFNGIPELPGPKSPTITTGTGEMVKQRGS
                     WTFLMQMASGTQSTQVAHWAFMQQCIAGSDQPCWVDDFPGKGVDQTIQELLAGCGAI"
     gene            complement(3698..4234)
                     /locus_tag="VLA60_03535"
     CDS             complement(3698..4234)
                     /locus_tag="VLA60_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08459.1"
                     /translation="MKHIISLGLHWREASHLLFGLSMLLVFILASGNDLYAGNRDHRD
                     GFSPKTNPSNYGHRYSKSILRGVRLSDVYTFNDTVTTAFGPAYANVWTSAANFLQCTP
                     PSGRPFFYALCYYSGPDAPTGNSDENPSLPCTLSPDGTLADVRVSKYLLMRYLLRCPT
                     SSIFMPTASFSPVAGPSN"
     gene            4664..>5150
                     /locus_tag="VLA60_03540"
     CDS             4664..>5150
                     /locus_tag="VLA60_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSF08460.1"
                     /translation="MGRDKIRIVGCVMNGQLFIESYGKVVMNRRIQEIEKPENRPGGN
                     CRLVSGLIGLLVCVGVLLINPGPGRAVVEEDVRALREDMEQVKKDLAEIKNILQSATK
                     RQRPDKSTGTVGVTEGAVLGEVDAPVTIVEFSDYQCPFCRRYSLTVFPILKREYIDTG
                     KV"
BASE COUNT         1276 a         1206 c         1300 g         1368 t
ORIGIN      
        1 gagcggcaaa tcggttccgc aaaacaggac gattgtatta aacaacaaac catgggtgtg
       61 tgtggtggta gaatctccgg attgtgaggc cgggctagta gatcgtgtca tcatcccctc
      121 ctgtcgaatt tctgaatgcg gaaggggagg cacgaggcca ggtaaccacc gccgacctag
      181 gatcagctag cccgttgcct ctcttgtttt ggtgcatcaa attaacaaac atcttcaatg
      241 aaaaatctcc cttttgtgat ggcaattggg gaggcaatta gaccatacca cttaaggtaa
      301 tacgaaacga caataaaacg actaaacgcc ttgtcagtat tgacgtttct gtgatgcggt
      361 ctagagtcgt gttgactggc gttgcaatat gtaatcacct ttgggagcag gatgtcggag
      421 gttcaaatcc tctcgccccg accaaatact ctgttgaaac cccttctcct cgtatcaccc
      481 ctcccaattt ttaacaacaa cctgggcgcc tgtcgcttag ttggatagac caaattgaac
      541 aaaccaggcg ttgtgtttgc atgactcctc ctgctttatc tcgtatgttg gggacgagag
      601 agatcgctcg ctcgcagtgg tctagctaat tgaggtagga tgacactgtc ctgacttgac
      661 cagccgtaat ttcatcatct gttggatctt gagggtacaa tccacacaca aaaacgctca
      721 ccactcatgt ctactatttc tactcatacc gttcggtcct ccattttcgc taagtatcaa
      781 ccgatcatcg atctcagcac caatcgtatc gttggctgtg aagcccttgc caggtggcga
      841 gcggatggtg gacgtgagat cagtatagaa cccgtcattg aagagttcga atcgcaggaa
      901 gacttagcct tagagttaac cacctgtatg tttgcctgtc ttaaagcgga tttaggcacc
      961 ctgctgctcg aacatcccac gttttcagtg agcgtgaatc tgccccccat tgtgatgggg
     1021 aaaaacaaga tcttgccggt gctggaacaa ttaggattga taccccatct ccaacagctc
     1081 accggggaaa tcactgaacg gcaagcactc aatgagaaag gccgggatgc catccgcctg
     1141 gctcggcaat tgggtgccag agtggccatt gatgacttcg ggactgggca ttcaggactg
     1201 caacaactca ttgacttgga agtggatacc cttaaaattg accaatcctt tattcagcag
     1261 ttgggaacca atgttgcggc ggaacgcctg gtgcgtggga ttgcagcctt ggccactatt
     1321 ctcgaggtgg atattgttgc cgagggtatt gagaccccag aacaagcgga ctttctacgg
     1381 gctattggga tcgaaaaagg acaaggatgg ttgtgggcga aagccctcga cgccaatgaa
     1441 ttatatgcgt ggttggacta acgctgcgct gtcatgaagg ttctatgtgt ggcttagcgt
     1501 tcgttgtcta gaaaagccag gtaatccaaa cctaaccatg taccttctta caagcctgac
     1561 tcctcagggg gggcaggctt tgttctttta ggcaaacata gagatggagt aaaaactaaa
     1621 gaaatatttg gctccctggc gcgtgaggga tccgatattc taaaagccag aggggcaacg
     1681 aagggtagtt tattgaggac aattttgatg aacaattagc catgtttttg taacgatgga
     1741 aagtcaaagg atcggatttt tgttacagtt aatctgtgca accaagcgtc atgtacgttc
     1801 ataagcatag agtcgtgaga ttggccggac cctaattttt atctaattat tttcaaatat
     1861 cggcggtgag gcctccatat cggtttttct ccagttgaaa aagaaaaatg ccgtataaac
     1921 agtttgcgtc accgatccta tctgcggctc ggttgagagc acaaaacgtc cggatgctct
     1981 ttcgaaaata tcaaaataaa agcgtacata gccctggctt tgcacacgtt tatagaaagc
     2041 caattcgggt gtggctatgg gaaatatcgt tgcctgagtg gccggcaacc ccaacaacct
     2101 tgcgttttgg gtcgtcccta tagactcaac gattaaatgc acacgatacg tcgcatcctc
     2161 ttgctcttcc cgaatgatca ctccctgttt tcctagccaa ccggcaacga cctgtctcat
     2221 atgctgactg atgatatttc cgtttaaact ctggtctacc ctcagatctg tagcggtcag
     2281 gagtattgat gctccttcag aatgtccaca tcataattct tcaggtcttt gttgattgct
     2341 gagctgaata acaattgctc ggtgggtgtc aggggatccg tcgccttctt tgagggtatg
     2401 gcgcagccgg acaggatcag ccaataaatc atgatggctc gcacggtctt taatctggtc
     2461 atcttccctt acttcctcat tttagagtga tggtctaacg aaagtttttc ctcaaaggaa
     2521 aacatttgca cgtgctagag tttcgaaggt ccttgatggg tgggataaca tacccgataa
     2581 aacactatta tcctgaaaaa gcagttagat catgaacgtc agcacaacgt ttattgtcta
     2641 tacatgcttt tgataaaccg atcttcgcta aatggcaccg cagcccgcga gcaactcttg
     2701 aatcgtttgg tcaaccccct tgcccggaaa atcgtcgacc cagcacggct gatctgagcc
     2761 ggcaatacat tgctgcataa atgcccaatg tgcgacctgg gttgattgag ttcccgatgc
     2821 catttgcata aggaacgtcc atgagccacg ctgtttgacc atttcgcccg tgccagtcgt
     2881 aatggttggg cttttcgggc cgggaagctc cgggatgcca ttgaaggaga tttccactcg
     2941 ccctcctggt tgacccggtg tactcggtgg tcccggtgtc accgagccga tcatgtattg
     3001 acagagagca ggctggcctt cgaattttac aaccacaggt ccgaagaaga atccgtcatc
     3061 gaatttctcg atatgccaga ccgtctggtc atcgattggc acagtcgtat ttggattgct
     3121 gggatcatcc gtgtcccacg tgtaggcctt gagatactca gatggcacgt acccgtaggt
     3181 gccgccgttt tctcccagcc aggcccactg agaagtttgt ggctccgatt taggactctt
     3241 cgccgcaact tcaggagata atcccatggt gatcatgatc aaacacgtca ccatgacaac
     3301 aagatatttc gatggtgaca tttcgctcac cagtcttttt ttcgagatac gtttttattt
     3361 gtgcttgatt ggataacgtc cctctacctc cagactatgt tcgcgtaatc atttatcctt
     3421 tctgaataaa tgaccgcgaa ttagttacca caaagggtgt gattaatttc gcattgggtc
     3481 ataatgccgg cgcctgtctg ttcggcagtc ggtgaggatt ataggaccac cggcagggtt
     3541 ataggctgcc gaccagacat tgtcgcctcg accatgcctc ttttgggtac cggaggaaat
     3601 ggtgccggga ggcgggtcgt ttgcgttgca actttggtct ccttgaccaa tttggaacgg
     3661 tccatcatag ctgcaggcct ctcccaataa ctgcgcttca gtttgatggt ccggcgacgg
     3721 gagaaaaaga tgcggtcggc atgaatatcg acgaggtagg acatcttagg agatacctca
     3781 tcagtagata tttcgaaaca cgtacatcgg ccaatgttcc atcaggggaa agcgtgcagg
     3841 gcaaagacgg gttttcatca ctattccctg tcggggcatc ggggcctgag taatagcaca
     3901 gggcataaaa aaatggtctg ccggatggcg gtgtacattg caggaaattc gctgcggacg
     3961 tccagacatt cgcatacgca ggaccaaagg ctgtggttac cgtatcattg aaggtataca
     4021 catcactgag tctcacgcca cggaggatcg acttactata tcgatgccca tagttgctag
     4081 gattggtttt gggagaaaac ccgtcacggt gatcccggtt gccggcatag agatcattgc
     4141 cactcgccag tatgaaaacc aaaagcatac tcaagccaaa gagcaagtgg ctcgcttccc
     4201 gccaatgcaa gcctaggctg atgatgtgtt tcatgatcaa cctccattca ggacattcct
     4261 aagccacaaa ctctagggct tcgatgtgct tccggttttt gtgaacgact ttcttggtag
     4321 atctctctac agcctgtatc ccttaaaacg gattcgttgg ctccggatcc gatgcggacc
     4381 aggatttttt gtctatacaa tatccatcat gctttgcctg ttggtccgtt gccgtttatc
     4441 tgcgattggt caacgcatct gatgtgatcc cttgcctgca cgttcatcta cctgatcgga
     4501 agaagccgga tgatttttgt tcggttaaca agaaaagtca aggaaattct cgtcgaactc
     4561 tgtttccagt ggttgcgcaa tccgtgttaa tcaatgtaac cgaatccctc gatcttctgg
     4621 ccgggacgtg ttaccttttc tttgggagtg ggttttgatc gaggtgggtc gtgataaaat
     4681 tcgcattgta ggctgcgtga tgaatgggca attatttatt gaaagttatg gaaaggtggt
     4741 catgaatcga cgaattcagg aaattgagaa accggagaac agaccaggag gaaattgcag
     4801 actcgtctca ggactgatcg gcctgctggt gtgtgtaggt gtgctcttga tcaaccctgg
     4861 accgggtcgg gccgttgttg aagaagatgt ccgggctctt cgtgaggata tggagcaggt
     4921 gaagaaggat cttgctgaaa ttaagaacat ccttcagagt gcgacaaaac ggcaaagacc
     4981 ggacaaaagt acaggcactg tgggtgtgac cgagggtgcg gtgctggggg aagtcgatgc
     5041 gccggtcacg atcgtggaat tctcagacta tcaatgccct ttttgtcgac ggtattcgct
     5101 aacggtgttc cctatactta aacgtgagta tatcgataca ggaaaagttc
//
LOCUS       DATIAL010000109         5748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_879053, whole genome shotgun sequence.
ACCESSION   DATIAL010000109 DATIAL010000000
VERSION     DATIAL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879053"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VLA60_03545"
     CDS             complement(<1..182)
                     /locus_tag="VLA60_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSF08461.1"
                     /translation="MWIFLNGEFVKKEQARISVFDHGFLYGDGVYETLRVYQGRIFLL
                     ERHLARLRRSCELIGLV"
     gene            complement(202..1710)
                     /locus_tag="VLA60_03550"
     CDS             complement(202..1710)
                     /locus_tag="VLA60_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HSF08462.1"
                     /translation="MSPISKHAERGVPSSVHSNPCFPLIQHCSPAFDDPFDLFGRVTG
                     YAPHSFFMEHDAVQEGVGHRYSYMGCDPYRVVRGKGHTYEIISVDKKETHIGDPFALL
                     QRTFVGQPVSSWSHLPPFQGGAIGCFSYDLVRMFEVLPERLRDDLHFPDMYFLFVEMF
                     VIVDHHAPGAWLIFSPSPDRLATENWDHLYREGQGRLSDLQAKVMIPENLPTKMHSAR
                     SSLCIEGEQSSLEYMDRVRACQQFIAAGDIYQANLSHRFRIEGVTHGFASQAEAGADL
                     YRQLRKVNPSPHSAFLVLESDVIVCNSPERLVRLSEGYADMRPIAGTRPRGREPHDDR
                     LLAEDLLSCPKERAEHLMLVDLARNDLGRVCDYGSVRVNELMTVERYSHVMHMVSQIS
                     GRLRDPYHGFDLIRATFPGGTITGVPKVHCMELIEQLEPVRRGIYTGSIGFIGWNGNL
                     DLNIAIRTLLLTEGHGLLHVGAGIVADSEPDREYQETLQKAEAFFQVLKGNG"
     gene            complement(1714..2298)
                     /locus_tag="VLA60_03555"
     CDS             complement(1714..2298)
                     /locus_tag="VLA60_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08463.1"
                     /translation="MMMKHFVLLLVMAVGTSGNSVWAISSGVDDLPDLTGGVVPVVQY
                     KQLDPFSGNDILTSVSEVEYTVKVKNQTGQPLIADSLVLVVDSVLEISGKEIINRVRI
                     EGSDGMTQDGKPFFRIPSTGPELSPYGESETVTIRVTNPDFLRFYPPGVRVRGIRRSS
                     EKAVKDLLDTLVQKGLLSPEDAMRALESPSSGSP"
     gene            2398..2700
                     /locus_tag="VLA60_03560"
     CDS             2398..2700
                     /locus_tag="VLA60_03560"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSF08464.1"
                     /translation="MGPLLLDMIQDFLLECDPFQRLALPIEKMAPSQDPMFAGLGQAM
                     RVGTDLLAALIVGGFLGWLIDSYVWDTTPWGVAIGLVLGLIAGVRNAYRAAQRWKQ"
     gene            2724..3449
                     /locus_tag="VLA60_03565"
     CDS             2724..3449
                     /locus_tag="VLA60_03565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSF08465.1"
                     /translation="MEDPLHPFELHNFLPLSIFGLDVSVNKAVLMMWVVVGLVAFILL
                     KAGGARALVPSKLQSLAELLVDFIRGIIHDTMGASGMRYFPLISALFLFILFSNLVGL
                     IPGSYTITSQIIVTGVFAVGVYLLSLIVGFQLHGAKFLGILVPPGTPGWLLPLMIPIE
                     LISQIARPISLAVRLFANMTAGHVILGVLFGLTIAGGLLIGWLPFSFTIALYGLEVGI
                     AFIQAYIFTVLTCVYIGDAMHLH"
     gene            3523..3750
                     /gene="atpE"
                     /locus_tag="VLA60_03570"
     CDS             3523..3750
                     /gene="atpE"
                     /locus_tag="VLA60_03570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HSF08466.1"
                     /translation="MDSAAAALLGMGLAAAGFAGAGIGIGYIFGKMIEAVARQPEAEA
                     RVGKYMWIGFALVEAIALYGLVIAFIIMGKG"
     gene            3765..4271
                     /gene="atpF"
                     /locus_tag="VLA60_03575"
     CDS             3765..4271
                     /gene="atpF"
                     /locus_tag="VLA60_03575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HSF08467.1"
                     /translation="MPQFDSHFFSSLIFWELLSFGILLWVLYKYALPPILETLENRER
                     KIRESLDQAEQNRLAAERKLKEYDAKLQVAAKEVETILAEAKQQAQRLLDENEQRLRA
                     ESQRIKEETTQDIERERRKAIQDIKTQSAELALMIAEKVIGRSLSDDDHRRYAEEALA
                     AVAAQSKN"
     gene            complement(4473..5300)
                     /gene="larE"
                     /locus_tag="VLA60_03580"
     CDS             complement(4473..5300)
                     /gene="larE"
                     /locus_tag="VLA60_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HSF08468.1"
                     /translation="MLSLEPTLQGKYEALQELFRHMGSVVVAFSGGVDSTLVLKVGFD
                     TLGAQTKAVTAISPTFPSLEVEEVRRLSTAIGAPLQFVETDQLQIDAFVKNDATRCFH
                     CKTDLYRLLSSLREAVGFQTIVDGTNVDDLGEDRPGINAARQLGVRSPLVEAGFSKAD
                     IRELAKGLGLANWNKPAAACLSSRITRGLPITKTYLSRVERAEAFLVAQGVTQVRVRL
                     HGDLARIEIDPTQMSILTQEPERSRLVEEFKKLGFQTVTLDLEGYRAGGGNLSSTNN"
     gene            complement(5287..>5748)
                     /locus_tag="VLA60_03585"
     CDS             complement(5287..>5748)
                     /locus_tag="VLA60_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HSF08469.1"
                     /translation="NLAISLNSTTNEQRSHLMPAVNGRYDLSELLNACREYTQFSERP
                     LTFEYVLLAGVNDSLDDARRLVRLLRGIRCKVNLIPFNEFPGNPFRRPSSEVIDTFQG
                     IVRSKGLDVFLRKSRGDDVLGACGQLGRSASEVLNQTTTNKKRSLPLYAQP"
BASE COUNT         1461 a         1562 c         1365 g         1360 t
ORIGIN      
        1 accaggccga tcagttcaca ggatcgtcga agtcgcgcca ggtgccgttc tagtaaaaaa
       61 attcgtccct ggtagacgcg cagagtttca tagactccat ccccgtacag aaacccatgg
      121 tcaaagaccg aaatccgagc ctgttctttt ttcacaaact caccgttgag aaatatccac
      181 atctcctatc cccgtttgtc gtcagccgtt ccctttcaga acttgaaaaa aggcttcggc
      241 tttttggagc gtttcctgat actcccggtc cggttcggag tcagccacga tccctgctcc
      301 cacatgaagc aacccgtgtc cttccgtgag caacaacgtg cgaatggcaa tattcaagtc
      361 aagattcccg ttccatccaa taaacccaat cgacccggtg tagattcccc gacgaacggg
      421 ctcaagctgc tcgataagtt ccatgcagtg gactttcggc actccggtaa tcgtccctcc
      481 gggaaacgtg gctcgaatga ggtcgaaacc atggtaaggg tcccgcaaac ggccggaaat
      541 ttgggaaacc atatgcatga catgggaata gcgctctacc gtcatcaatt cattgacccg
      601 aacggatcca taatcgcaca cccgccccag atcatttcga gccaaatcaa ccaacatgag
      661 atgctccgcc cgttccttgg gacaagacaa cagatcttcc gccaggagtc gatcatcatg
      721 aggttctctg ccacgaggcc tggttcccgc gattggacgc atatctgcat atccctctga
      781 aaggcggact aatcgttccg gggaattaca cacaatgacg tcggactcca acacgaggaa
      841 tgccgaatga ggagagggat taaccttgcg caattgccga tacaagtcgg ctccggcttc
      901 ggcctggctt gcaaaaccgt gagtcacgcc ctctatccgg aatcgatgag agagattggc
      961 ctgataaata tctcccgccg caatgaactg ctggcaggca cgcacgcgat ccatatactc
     1021 cagagacgat tgctccccct caatgcaaag cgaggacctg gcggagtgca tcttcgttgg
     1081 gagattttca gggatcatca ccttggcttg cagatcagac aggcgcccct ggccttcgcg
     1141 atacaagtga tcccagtttt cggtcgccag gcggtctggc gaaggcgaaa atatcagcca
     1201 ggcccctggc gcgtgatgat ccacaatgac aaacatctcg acaaacagaa aatacatatc
     1261 aggaaaatgc agatcatccc gtaggcgttc tggtaacacc tcaaacatgc gaacaaggtc
     1321 gtagctgaaa cagccgatcg ctcctccctg gaaaggcgga aggtgggacc acgaactgac
     1381 gggctgacct acaaaggtac gctgcaacaa cgcgaatgga tccccaatgt gcgtttcctt
     1441 cttatctacc gaaataattt catacgtgtg ccccttcccc cgaaccacgc gatagggatc
     1501 gcaccccata taggaataac gatgccctac tccttcctga acggcatcat gttccatgaa
     1561 aaaggaatgc ggagcataac cggtcacccg gccaaagagg tcaaagggat catcaaaagc
     1621 aggggaacaa tgctgaataa gcggaaaaca cggattcgaa tgaaccgagg aaggcactcc
     1681 ccgttcggca tgcttggaaa tgggggacaa gggttaagga ctacctgatg aaggagattc
     1741 caacgcccgc atggcgtctt cgggagacaa caaccccttt tgcaccaggg tatcgagtag
     1801 atctttgacg gctttttcag aagagcgccg aatcccgcga acccgaacac caggaggata
     1861 aaatcgaaga aaatcaggat tcgtcacccg tatggtgacc gtctcgctct ctccataggg
     1921 tgacagttca ggcccggtac tgggaatcct gaaaaaaggt tttccatcct gggtcatccc
     1981 atcggaacct tcgattctga cacggttgat gatctctttt ccggaaattt ccaggacgga
     2041 atccaccacc aggaccaagg aatctgcaat taacggctgg cccgtttggt ttttgacctt
     2101 caccgtatat tcaacttcac tcactgaggt taaaatatcg tttccagaga aggggtcgag
     2161 ttgcttatat tgcacgacag gcaccacccc accggttaaa tccggcaaat catcaacccc
     2221 ggatgaaatg gcccacacgg agttcccact cgtgccaacc gccatgacca ggagtaacac
     2281 aaaatgtttc atcatcatga atgttggcca aaaatgaacc cggaactata cccgcgagga
     2341 gacttcatgt ttttttcctt tgtccctgac tgtaccttac cataacaatc ttgagaaatg
     2401 ggacctttgt tgcttgacat gattcaggat tttttgcttg aatgtgaccc cttccaacga
     2461 ctcgccttgc cgattgaaaa aatggcacct tctcaggatc caatgtttgc ggggctggga
     2521 caggcaatgc gagtggggac ggaccttctt gccgccctta tcgtcggtgg attccttggt
     2581 tggttaattg attcgtatgt gtgggatacc acgccgtggg gtgtggcgat aggactggta
     2641 ctgggattaa tcgctggagt tcgcaacgcc taccgtgcag ctcagcgttg gaaacaatga
     2701 gtcgctagac acaaggttta gttttggaag atccgttaca tcccttcgag cttcataact
     2761 ttctcccgct ttccattttc ggccttgacg tttccgtcaa taaggccgtc ttgatgatgt
     2821 gggtcgtggt gggacttgtg gctttcatat tattgaaagc cggtggcgcc agggcactcg
     2881 tgccttctaa gctccaaagt cttgcggagt tgctggtcga cttcattcgc gggattatcc
     2941 acgacacgat gggagcctcc gggatgcgat atttcccgct catcagcgcc ctctttctgt
     3001 ttatcttatt ttcaaacctg gtcggtctta tacctggatc ctacacaatt accagccaaa
     3061 ttattgtgac cggggtgttc gctgtagggg tctatctact cagtcttatc gtcggatttc
     3121 aactgcatgg ggcaaaattc ctgggcatac tcgttccccc tggcacgcca ggctggctcc
     3181 ttcccttaat gattcccatt gaattaatta gtcaaatagc tagaccaatc tcactggccg
     3241 tccgattatt cgccaatatg actgcaggcc atgtgattct tggggtattg ttcggcctca
     3301 ccattgccgg cggcctgctg ataggatggc tgcctttttc ttttaccatc gcactctatg
     3361 gattagaagt cgggattgcc tttattcagg cctatatttt taccgtttta acctgcgtct
     3421 atatcggcga cgcgatgcat ttacactaac tgacattcga acgaaacgat cagaggacgt
     3481 ttttacaatt agtccctttt aaactgggag gacaacacta caatggattc tgcagcagca
     3541 gcacttttgg gaatgggcct ggcagcggcg ggctttgcgg gtgccggtat cggcatcggg
     3601 tatatttttg gaaaaatgat tgaggcggtt gcccgccaac cagaggcgga agctcgagtc
     3661 ggcaagtaca tgtggatcgg gtttgccttg gtcgaagcca tcgcgctcta cggactggtc
     3721 atcgccttta tcatcatggg caaaggataa agggctcctg acctatgcca caatttgact
     3781 cacatttctt ttcgtcgctg atattttggg aacttctgtc atttgggatc ctcctatggg
     3841 tgctctataa gtacgccctc cccccgatcc tggaaacact ggaaaaccgg gaacgaaaga
     3901 tcagggaaag cctcgaccag gccgaacaaa accggcttgc agccgaacga aagctcaagg
     3961 aatacgatgc caaactgcag gtggccgcca aagaagtgga aacgatcctg gcagaggcca
     4021 agcaacaagc tcaacgactg cttgatgaaa atgagcaacg gcttcgtgcc gaatcccaga
     4081 gaattaaaga ggaaacgaca caagacattg agcgggaacg acgtaaggcc attcaagaca
     4141 tcaagaccca atctgctgag ctggccctca tgattgcgga aaaggtgatc ggtcgaagcc
     4201 tatcggacga tgatcatcgg cgatatgccg aagaagcgct cgcagccgtc gctgcccaat
     4261 caaaaaacta agactttccc cattctctcc gaatagcctc aagtacagag ccattctttc
     4321 aagattcgcc aacatcaaaa ggccacgcac aataaaaaag agggaagatt cacttccctc
     4381 tttttttatg gtgttcccaa gaacacctac aaaccacggt ttttgagtaa gagactccca
     4441 gtgacccgct cccaaaaatc atgatctacg cttcaattgt tcgttgagga aagattccca
     4501 cctccggccc gatacccttc taagtccagc gtgaccgttt gaaatcccag cttcttaaat
     4561 tcctccacta aacgggatct ttccggttct tgtgtcaaaa tcgacatttg ggttgggtca
     4621 atctcgattc tcgccaaatc gccgtgaagg cgaacccgca cttgcgtcac tccttgagcc
     4681 accaaaaagg cctcggcacg ctctacccgg gaaaggtagg tcttcgtgat gggaagacca
     4741 cgcgtaattc gtgaagacaa acaggcggca gccggcttat tccaatttgc caaccccaaa
     4801 ccttttgcta attcccgaat atccgcctta ctgaatcccg cttcaaccaa aggacttcgc
     4861 acgccgagtt gtcgggccgc attgataccg ggacgatctt ctcccaagtc atccacatta
     4921 gtcccgtcta cgattgtctg aaatcccacg gcctcccgaa ggctggaaag caaccggtat
     4981 aagtctgttt tgcaatgaaa gcaccgggtt gcatcatttt tgacaaacgc atcaatttgc
     5041 aactgatccg tttccacaaa ttgcaagggc gccccgatcg ctgtgcttaa tcgcctgacc
     5101 tcttccacct ccagtgatgg aaatgttggc gaaatcgccg tgaccgcttt ggtctgcgca
     5161 ccaagtgtgt caaagcccac ttttaacacg agggtgctgt cgaccccgcc agaaaacgcg
     5221 actacgaccg atcccatatg gcgaaagagt tcttgcaagg cctcatactt cccctgcaga
     5281 gttggttcaa ggctgagcat agagagggag actccttttc ttgttcgtcg tcgtctgatt
     5341 taagacttcg gaagctgatc gacccaattg gccgcaagct cccaacacat catctccccg
     5401 gctttttcgg agaaacacat ccagaccttt gcttctgaca ataccttgaa aggtatcaat
     5461 aacttctgat gacggacgcc gaaacggatt gcccgggaat tcattaaacg gaatcaaatt
     5521 caccttacac cggattcccc gtaaaagccg caccaaacgc ctggcatcat ctagagagtc
     5581 attgaccccc gctaacaaca catactcaaa cgtcaggggg cgctcgctga attgagtata
     5641 ctcgcggcag gcattgagca attcggataa atcatagcgc ccattcactg caggcatcag
     5701 atgtgaccgc tgctcattgg ttgtgctatt taaagatatg gccagatt
//
LOCUS       DATIAL010000110         6549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_19115, whole genome shotgun sequence.
ACCESSION   DATIAL010000110 DATIAL010000000
VERSION     DATIAL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19115"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VLA60_03590"
     CDS             complement(<1..561)
                     /locus_tag="VLA60_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08470.1"
                     /translation="MNYTQPMFSGIPTHAYPGAGAFSGPHFQHGGTGFCPGCSHPRAK
                     CCCGHRECRKEAKELLVEADISVDGKTDPKNSMVLQRVRAMAMFVAPVEMSAGETSGK
                     NAETKAAVAPIVGDVDVRGKAGIGKAFIGGGCCVHLSIEYTLTNAASTAPALVFVVVQ
                     DSEKTTLGWMKQAGPHDGYQIKEGIIT"
     gene            complement(646..1122)
                     /locus_tag="VLA60_03595"
     CDS             complement(646..1122)
                     /locus_tag="VLA60_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08471.1"
                     /translation="MHHHLPHCCVSTSHHSSPHYHTTGHRSMVSVGYPVLMPMPYYPA
                     VGYPAYGWGTQATHSMPLPQEVSVDSATGMKDVLIGGTNNASLTLEYAPDAGATSPLV
                     KVTVDIDGSISTWQETSMTEGYHVKSHLLSIEPGARVTLEVTEAMARLRWCETICC"
     gene            complement(1122..1316)
                     /locus_tag="VLA60_03600"
     CDS             complement(1122..1316)
                     /locus_tag="VLA60_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08472.1"
                     /translation="MATVIESVNKLINQTSTQILENHIPQKMADMVNTVVNTGFQIVE
                     DVLKITQDLTKASDSNQGDS"
     gene            complement(1360..2820)
                     /locus_tag="VLA60_03605"
     CDS             complement(1360..2820)
                     /locus_tag="VLA60_03605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF08473.1"
                     /translation="MTYDVIVVGAGPGGSSTAAFTARKGLSTLILDRTDFPRDKVCGD
                     GLTPQALYWLDQLGCIDEVLTHTKSCIKTCDLFINGELLLTGGFPNTTHYPDFCVLLD
                     RRRFDHILLQHAVASGAEFQAYSLVREIHQTPDHARVVLDHKGQRMEVQGKIVIGADG
                     VSSVVSRSIGNVLKNGATALSLRAYYQNVEAEGAQIKVYFDERFFPGYAWVFVDDDGF
                     ANIGLGYAFDKPFPMIPNLKQVFQDFLDQDLGHLLRHASRCGAVSGGAVSFFKPKAIV
                     ADRVMLVGDAANQADPLNGGGIHKAMEGAYLAAETAHQAVLSGDCSVRALQQYQTQWE
                     QQFELDWRTGELFLSVAKNPDLKEFCLFVLANIARMTTHDRQFQEFCSGVFSGVLSQN
                     ICLVPQALFHAMPRNPAAWMAMLQLDEKGLVTGPLSLAIDALTSSAKASSRMLMSPWQ
                     SLNWSLEVMTKVLRLARGHAEGSFTSTGSALAKCPI"
     gene            complement(2881..3609)
                     /locus_tag="VLA60_03610"
     CDS             complement(2881..3609)
                     /locus_tag="VLA60_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08474.1"
                     /translation="MKSESKATASKNSKKKSPSRRSSTPKKSTAQASAHTDQNTKSED
                     SMFGHGDQIGTTFAKGLDLAEAGLSLGLTLINRFGTMMQDSVLEKFSGVMGAATASPQ
                     ESTPSSSRSHDSTAPGPSSEHSESIEPQDPAYYIANRLPLIPGQSVHVSFSINNDSMD
                     SAKNVKLKVDGFVGSTQGQVFPCKGFTVSPGTKTIDPMDFEKFILKGVIPAGLSPDIY
                     HGRIVVMSEQAIEIPVKLLVTSAT"
     gene            complement(3920..4684)
                     /locus_tag="VLA60_03615"
     CDS             complement(3920..4684)
                     /locus_tag="VLA60_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08475.1"
                     /translation="MGDIKIKEAKNLDKLRISELSNVDPLVISEIRHIAPAAVHIKEL
                     NHIDPLTIESLRINEIRNLDPIRVEQFNVTNLPTVNLSLRQLPAVDMNIRRLPPVSIG
                     LHQDFCLPSSYTLRARVLGIEFLRLNLEGRTMVSPRDRVRREQSRIPAQSFPQVATAG
                     NPGIPSRSVTKGSRVVVTGSPCQSMPSSHQSKPGTSAGGPISREGFRPGGEYGGGRQA
                     SSGGLKVGNVPSPTFPMAKAGSQRDFMDSAVSSGEN"
     gene            complement(4709..5443)
                     /locus_tag="VLA60_03620"
     CDS             complement(4709..5443)
                     /locus_tag="VLA60_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08476.1"
                     /translation="MIEKGLSVSVHPAPCRHSHLTYQVYHQVVCQHQIEVEVWPANAD
                     VYFRDERYADPVNTQVRFHASVYNGMSSKVNWAVQDPAGNPGAGSIDPTGLYLAPPKG
                     SLSHGTTDIIIATAAESPFRKAYAFVTLMGNGPEPLPQPILEIFPKQSYLYYPQGHDN
                     AYIDQSNTQQLFRATIRHSSGTAVQWLVDSVVQSGAGTVPWFLYQLTGSGASKVVTIS
                     ARLQTQPAILDEAKAIHLNYSWPGLV"
     gene            complement(5460..5888)
                     /locus_tag="VLA60_03625"
     CDS             complement(5460..5888)
                     /locus_tag="VLA60_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08477.1"
                     /translation="MNFKGHLGMESCPDFSVQAKTEPIAFATKAKGSLVAQVSAISVR
                     VGEIPINLSIPFLRRAGGIHTVASIGSFGIKLSPFTVAVEGANVQLAGVLGTKGITTQ
                     LEGKVGCKSKISFNGKLFGKIANCSVDLPDDDFEEHVDTM"
     gene            complement(5885..6130)
                     /locus_tag="VLA60_03630"
     CDS             complement(5885..6130)
                     /locus_tag="VLA60_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08478.1"
                     /translation="MGTPVSFFTKGDKMAANMVLRIKLGPFVSFEIEGENCEEICQSL
                     KGFEKLNERIDAMCSDLAERVYPDGMTAQQTGQEEAT"
     gene            complement(6194..>6549)
                     /locus_tag="VLA60_03635"
     CDS             complement(6194..>6549)
                     /locus_tag="VLA60_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08479.1"
                     /translation="ISILDQFGTVLRERGKEYRTSSDGQELERKLEDLYQNLDRQQKD
                     TIEVMLHSMDQMVKFLREADRNLSQVHDKPMNGHVQTNGHGMAKKKANGTGAMKAGNG
                     NGKRPVRRQDSRKSK"
BASE COUNT         1583 a         1789 c         1586 g         1591 t
ORIGIN      
        1 ggtgataatt ccttctttaa tttgatagcc atcatgaggg ccggcctgct tcatccagcc
       61 tagagtcgtt ttttcgctgt cctgtacgac cacaaaaact aaagcgggtg ccgtcgatgc
      121 cgcattcgtc agggtatatt cgatggaaag atgcacgcaa catcctcccc cgataaaggc
      181 cttgcctatt cccgcttttc ctcgcacatc cacatcaccg acaatcggag ccacggctgc
      241 cttcgtctct gcgttcttgc cacttgtttc gccggctgac atctcaaccg gagctacaaa
      301 catggccatt gcccggactc gttgaaggac catggaattt ttgggatccg tctttccgtc
      361 gacactgatg tccgcttcaa caagcaattc cttggcttcc ttccggcatt cccgatgccc
      421 acagcaacat ttagccctgg ggtggctaca accaggacaa aacccggtgc caccatgctg
      481 aaagtgtggt ccggaaaagg ctccggcacc aggataggca tgagtgggaa ttccagaaaa
      541 catcggctga gtgtaattca ttggtgtgcc cttccttaat cttgcctcaa attaaataac
      601 agctcaaaaa gacaatacgc ctatgcttga ggtctcctca tccgatcaac agcagattgt
      661 ttcgcaccag cgcaatcggg ccatcgcttc tgtcacttcc aacgtcaccc tggcccccgg
      721 ctcgatggat agcaaatgcg atttcacatg atagccttcc gtcattgagg tttcctgcca
      781 ggttgagata ctcccatcaa tgtccaccgt gaccttgacc aagggggaag tcgccccggc
      841 atccggcgcg tattccaggg tcaatgaggc gttgttggtt cctccgatca acacgtcttt
      901 cataccggtt gcgctgtcca cactgacttc ttgaggaaga ggcatggaat gggtggcctg
      961 ggttccccag ccgtatgcgg gatatccgac tgccgggtag tatggcatcg gcatcaggac
     1021 cggatacccc actgaaacca tgctgcgatg accggtcgta tgataatgcg gggacgaatg
     1081 gtgagacgtc gagacacagc aatgcggtaa atggtgatgc attaggaatc tccttggtta
     1141 gagtccgatg ctttcgtcaa atcctgggta attttcaaca catcttccac aatctggaaa
     1201 cccgtattga ccacggtatt gaccatgtcg gccatttttt gagggatgtg attctctaga
     1261 atttgtgtgg aagtctgatt gatgagtttg ttgacggatt ctataacggt ggccatgtcg
     1321 tgtacctttc tccggacggg ttggaatccc tgccggtgat caaattgggc attttgccaa
     1381 tgcggagccc gtcgacgtaa aggatccttc ggcatggccc ctggccagcc gaagaacttt
     1441 ggtcatcacc tcgaggctcc agtttaagct ttgccaggga ctcatcaaca tgcggcttga
     1501 cgctttcgca ctactggtca atgcgtcgat ggccagactc aggggtccgg tcaccaaccc
     1561 tttttcgtct aattgtaaca tagccatcca ggccgccgga ttgcgtggca tcgcatgaaa
     1621 caaggcttgc gggacaagac agatattctg agaaaggacc ccactgaaca ctccactaca
     1681 gaattcttga aattgccggt catgcgtggt catccgagca atgttggcaa ggacaaacag
     1741 gcagaattct tttaaatcag gatttttggc tacggataaa aataattccc ccgtccgcca
     1801 gtctaactca aattgttgtt cccattgcgt ttggtattgt tgaagagcac ggacagaaca
     1861 atccccgctc aacacggcct gatgcgcggt ttcggcagct aaatacgccc cttccatcgc
     1921 tttatgaata cctccgccat tgagcggatc ggcttgattg gccgcgtcac ccaccagcat
     1981 cacccgatct gccacgatcg ccttgggttt aaaaaatgag acagccccac ctgaaacggc
     2041 tccgcaccgt gaggcatgac gcaagaggtg gcccagatcc tgatccagaa aatcctggaa
     2101 cacctgcttc agattcggga tcatgggaaa cggcttatca aacgcgtacc ccaacccgat
     2161 attggcaaag ccgtcatcgt ccacaaagac ccaggcatac ccgggaaaaa aacgctcatc
     2221 gaaatacacc ttgatttggg cgccttcagc ctccacattt tggtaatatg cacggagtga
     2281 aagggccgtc gctccatttt ttaagacatt cccgatagag cgggacacga cggaactcac
     2341 gccgtctgcc ccgataacaa ttttcccctg tacttccatg cgctgtcctt tatgatccag
     2401 gacgacgcgg gcatgatcgg gggtctggtg aatctctcga acaagggagt aggcttggaa
     2461 ttcggctccg gaagccacgg catgttgaag aagaatatga tcaaaacgcc ggcgatccaa
     2521 caacacgcaa aaatcaggat agtgagtggt attcggaaat cccccggtca gcagtaattc
     2581 gccgttaata aagaggtcgc acgttttaat gcaggatttg gtgtgagtca gcacttcgtc
     2641 gatacagccc aactggtcca gccaatataa agcttgaggg gtcaacccat ccccacagac
     2701 cttatcccgg ggaaaatcgg ttcgatcaag gattagggtc gagagcccct tccgtgccgt
     2761 aaacgccgcg gtgctgctcc ctcccggtcc ggccccaacc acaatcacat cgtacgtcat
     2821 aggttctccc gtcaaatatc cctatgggta gcataaccct tattcgagag cttctcccct
     2881 ttacgttgcc gacgtcacca ataatttcac cgggatttcg attgcctgtt cggacatcac
     2941 aacaatacgt ccatgataaa tgtccggcga aaggccggcg ggaatcacgc ccttcaaaat
     3001 aaatttttca aaatccatcg gatcaatcgt tttcgttcca gggctgaccg tgaagccttt
     3061 gcaaggaaat acctgtcctt gagtcgatcc gacaaatccg tcgaccttca gcttgacgtt
     3121 cttggcggag tccatcgaat cattattaat agaaaatgac acatgcaccg attgcccagg
     3181 aataagcggt aaacgattcg cgatgtaata ggcgggatcc tgcggttcga tactctcgga
     3241 atgttccgag gaaggccccg gggcagtcga atcatgtgat ctggaggacg agggagtaga
     3301 ttcctgaggt gaagcggtgg cggcgcccat gaccccggaa aatttctcca gcacggaatc
     3361 ctgcatcatc gttccaaaac ggttgatgag ggtcaagccc aaacttagac ccgcttcagc
     3421 aagatccaat cccttcgcaa acgtcgtgcc gatttgatcg ccatgcccga acatcgaatc
     3481 ctccgatttg gtgttttggt ccgtgtgagc ggaagcttgg gctgtcgact tttttggagt
     3541 tgaagatcgc cgtgacggag actttttctt actgttttta ctggcagtag ccttgctctc
     3601 ggacttcatg ctcatgactc caaagacaat atgcgaatta acaacatgaa tggcacgcac
     3661 aaggtgaagg ttggcactgc acacaataat ggtgctggac aaccggtcca cacccacgac
     3721 gcatcgtaca ggcgcatccg tgtgaacatg gtggctccag ccagatgagc ccgtgcatga
     3781 attgccggaa caattgggca ccagcaccga ccagatggaa ggccaggcca atagcctgac
     3841 cagtggtctg tcccattaca gtagctccat cgtgtccatg ctttgagtat gagcattgcg
     3901 aatgtacaaa ggacataggt taattctccc cgctgctgac agcactatcc atgaaatccc
     3961 tctgagaccc ggccttggcc atcggaaagg taggagaagg gacatttccc actttcaatc
     4021 cacccgaaga cgcttgtctc ccccctccgt actcacctcc aggcctgaaa ccctctctgc
     4081 ttataggtcc ccctgcagac gtcccgggtt ttgactgatg agacgaaggc atggactgac
     4141 aaggtgatcc cgtaaccacg acccgtgagc ctttagtaac cgatcgagat ggaatacccg
     4201 gattgccggc tgtcgccacc tgagggaagc tttgcgcggg aatacgcgat tgctcacgcc
     4261 tgaccctatc ccttggggaa accatggtcc gcccctccag attaaggcgg aggaattcaa
     4321 tccctaacac ccgtgcgcgt aatgtgtagc tggagggaag acaaaaatcc tgatgaagtc
     4381 cgatcgatac aggaggcaag cgccggatat tcatatcaac cgcagggagt tgccggagag
     4441 acaaattgac ggttggcagg ttggtcacat tgaactgttc aacacggatc ggatccaggt
     4501 tgcgaatttc attgatgcgg agggattcaa tcgtcagggg atcaatatga ttcagttctt
     4561 taatgtgaac ggcggccggg gcaatatgtc gaatttcgct aatcacaagc ggatcaacat
     4621 tactcagttc actgatccgc aatttatcaa gattttttgc ctctttaatt tttatgtcgc
     4681 ccatatggat gcccttccct ggcggatgct acacgagtcc tggccagcta tagtttaggt
     4741 gaatcgcctt ggcctcatcg agaatggccg gctgggtttg taatcgggct gaaatcgtca
     4801 cgactttgga agcacccgac cctgtaagct gataaagaaa ccagggaaca gtgcccgccc
     4861 cgctctgcac caccgaatcc accagccact gaactgccgt ccctgaactg tgccgaatcg
     4921 tcgcccgaaa taattgttgg gtgttgctct gatcgatata ggcgttatca tgaccttgag
     4981 gataatagag atacgactgt ttggggaaaa tttcaaggat tggttgagga agcggctcgg
     5041 gcccatttcc catcaacgtg acaaaggcat aggctttccg aaaggggctt tccgccgctg
     5101 tggcgatgat aatgtcggtt gtcccgtgag agaggctgcc tttcggagga gcaagatata
     5161 agccggtcgg atcaatcgaa cccgccccgg gatttcctgc aggatcctga acagcccaat
     5221 tcaccttact gctcatcccg ttatacactg aagcgtgaaa ccggacctgc gtattcacag
     5281 gatcggcata gcgttcatca cgaaaataca cgtcagcgtt ggccggccag acctccactt
     5341 cgatttgatg ctgacagacg acctgatggt agacctggta agtcagatgg gagtgacggc
     5401 atggtgcagg atgaactgac acggacagac ctttctcaat catgttctca cgatgatagc
     5461 tacatcgtgt cgacgtgttc ctcaaagtca tcatccggaa gatccacgga acaattggca
     5521 atcttcccga agagtttgcc attgaagctt attttggatt tacatccaac cttcccctcc
     5581 agttgtgtgg tgataccttt tgtgcccagg actccggcta actgcacatt ggccccttca
     5641 acggcgacgg taaatggaga aagtttaata ccaaaactgc caatcgaagc caccgtatgg
     5701 attccaccgg cacgccgaag aaagggaatc gacagattga tcgggatctc ccctacccgt
     5761 accgagattg cactcacctg tgcgaccaac gatcctttgg ccttcgtggc aaaggcgatg
     5821 ggttcggttt tagcctggac agaaaaatcc gggcaactct ccatacccag gtgaccttta
     5881 aaattcacgt cgcctcctcc tgtcctgtct gttgagccgt catcccatcc ggatagaccc
     5941 gctcggccag atcactacac atcgcatcaa tccgttcatt taatttttca aaccccttca
     6001 aggattgaca aatttcttca cagttctccc cttcaatctc aaacgagaca aagggaccga
     6061 gtttaattcg aaggaccata ttggccgcca ttttatcacc tttggtaaaa aatgaaaccg
     6121 gggtacccaa attagggcgt tcccttcaat accttgcttc ccctgaattt gacgtggcca
     6181 tactcaccct tgattatttc gacttccttg aatcttggcg acgcaccgga cgcttgccgt
     6241 ttccattccc ggctttcatc gctccggtcc cattggcctt ctttttcgcc attccatggc
     6301 cattcgtctg cacgtgacca ttcatcggct tgtcgtgcac ctgactcagg tttctatccg
     6361 cctctcgcaa gaatttcacc atctgatcca tggaatgcaa catcacttca atcgtgtctt
     6421 tttgttgtcg atcaaggttt tggtataaat cttcaagctt tcgttcaagt tcctgcccgt
     6481 cactggaagt gcgatattct ttgccacgtt cccgcagaac agttccgaac tgatccagaa
     6541 tggagatca
//
LOCUS       DATIAL010000111         7221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_449397, whole genome shotgun sequence.
ACCESSION   DATIAL010000111 DATIAL010000000
VERSION     DATIAL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449397"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VLA60_03640"
     CDS             complement(<1..912)
                     /locus_tag="VLA60_03640"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HSF08480.1"
                     /translation="MNEKKIRVLIVDDSALIRNVMTEILAQDPEIEVVGTAPDPYAAR
                     DKMKALNPDVLTLDVEMPKMDGLTFLQKIMAARPMPVVMVSSLTEQGAATTLQALEAG
                     AVDFVTKPTVDIQHGLSDLAHQIISKVKIAALASVKKRTPPADCTERIKALAAQSAMI
                     KTTDTIITIGASTGGTEALRELLEVLPPNTPPIIMTQHMPEQFTKSFANRLNELCQIH
                     VKEAQEGDSVLPGQALLAPGNYHMELRRSGARYYVSLNQAPPVNRFRPSVDVMFRSVA
                     RFAGGNSLGVILTGMGNDGAAGMLEMKQ"
     gene            complement(1230..1838)
                     /locus_tag="VLA60_03645"
     CDS             complement(1230..1838)
                     /locus_tag="VLA60_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08481.1"
                     /translation="MSSTGLMEFGHIRRMQDTRFPYEVAVILPGEYFVSREPKVIYTV
                     LGSCISVCLRDPLAGVGGMNHFMLAAPSNTEGHDNWADSGRYGSFAMEMLMNDIFKRG
                     GKKERLEAKVFGGGKIYDGTIDIGAQNAAWALAFLEQEGLSPIKADVGDVCPRKVYYF
                     TDSGKVLMKKLDRIVASEIAKEEGQYRKKLQHAPVQADVTLF"
     gene            complement(1919..2746)
                     /locus_tag="VLA60_03650"
     CDS             complement(1919..2746)
                     /locus_tag="VLA60_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HSF08482.1"
                     /translation="MLTDKEFELFKHLIYQQVGIKLDEPKKTLLVSRLGKRLRDLHLS
                     SYQAYYDCVSGKGGEEELIKLLDLVSTNKTEFYREPVHFDFLRDQVLPEVQSSKTLRI
                     WSSASSSGEEPYTIAMTLSDAITDINRWDIKILASDISTRVLAKASSGIYEEERVSQL
                     PNDLVKRHFLRGKGPQAGKLQVRPQVAHLVAFRRINLMDPTFPIRSQLDVIFCRNVMI
                     YFDRPTQAKLMEKFFRYLRPGGYLFIGHSESLQWIDHQFTYLRPTIYQKPVGVNSKA"
     gene            complement(3785..4549)
                     /locus_tag="VLA60_03655"
     CDS             complement(3785..4549)
                     /locus_tag="VLA60_03655"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSF08483.1"
                     /translation="MRAFVSLLFFAVVQFGLSGISWSADASIFGTFALFEVYKDWGNV
                     SAEDRQKGGEEAKALLESFKGQVTVDGYWTYGLTTDSHFMVRLHSADLKANQQLLTQL
                     RSTGLGRHLDLEYTISGMTKGLNYAPDFPELLEKLKAAKYEGDPSTYAIMIPIRKNAE
                     WWNLPNEERVALMKEHTIPTVAYLKTVKRKLYHSTGLSDVDFLTIFETNNLKEFNDLV
                     IALRMVREDTFNVQLGEPTIIGTLKNWNDIVDLLVK"
     gene            complement(4740..5366)
                     /locus_tag="VLA60_03660"
     CDS             complement(4740..5366)
                     /locus_tag="VLA60_03660"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HSF08484.1"
                     /translation="MVTGRTSVFSCAANFLRGRKCIFCGSFQVLHTNRGYVKCQLCGK
                     SKSLRRLRREITILQGFYLLQPAYRLSRDLAVDSKVVTRVYQRMREALYHLAELEGGK
                     LKREIELDEASFGGRRKGKRGRAAAGKSVVFGLLERENRVYTKVVESVSPEELMRHIQ
                     AKTRKGSVYYTDAFRGYQSLKRYGKHQTINHAKTFVRGRRVKNHINGI"
     gene            complement(5481..6449)
                     /locus_tag="VLA60_03665"
     CDS             complement(5481..6449)
                     /locus_tag="VLA60_03665"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HSF08485.1"
                     /translation="MRSFTHLTLEQRYQIQALMKMGHSYQEIGGVIGVHKSTITREVR
                     RNQGRGGYRPQQAHRFTLSRRRTKVRRRIAAATWHCVNVLLKAEWSPVQISGWLRTQQ
                     GTLISPEWIYQHVYRDKRARGTLYRHLRCQRHSRKRYGTYSRRGHIPNRLSIERRPAV
                     VERRSRLGDWEVDTMIGKGHQQALVSLTERKSRLSLIAKVARRGANTVATAVINLLKP
                     LGLPLHTVTSDNGKEFAHHAIMVQALKVRFFFAHPYAAWERGLNENTNGLIRQYFPKQ
                     RPFTTITQQEIQQVMNKLNNRPRKSLGFKTPNQVCFGIHPIVALTS"
     gene            complement(7006..>7221)
                     /locus_tag="VLA60_03670"
     CDS             complement(7006..>7221)
                     /locus_tag="VLA60_03670"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF08486.1"
                     /translation="FGDSAYPRQGDKIASEATGGDVRVMLPLFYGKGCMGCHGGPKGE
                     MDVSGYKKEGAKEGDLGGAISVKLPAK"
BASE COUNT         1834 a         1955 c         1582 g         1850 t
ORIGIN      
        1 ctgtttcatt tccaacatac cggcagcgcc gtcattcccc attccggtca ggatcacccc
       61 gagactattc cccccggcaa acctcgcgac cgatcgaaac atgacatcta ctgaaggacg
      121 aaatcggttg accggtggcg cttgattgag agagacataa tagcgagcac cgcttcggcg
      181 aagttccata tgataattac ccggcgccag caaggcctgt ccgggcagga cactgtcacc
      241 ttcctgagct tctttcacgt gaatctggca aagctcattt agccggttcg caaacgattt
      301 cgtaaattgc tccggcatat gctgcgtcat aattatgggt ggagtattcg gtggcagcac
      361 ttccaataat tcccgaagcg cttcggtccc acccgttgac gcaccaatgg ttataatcgt
      421 gtcagtggtt ttaatcatcg cggattgagc agccagagct ttaatccgtt cagtgcagtc
      481 agcaggtgga gttcgttttt tcacgctggc tagggcggcg atcttcactt tgctgataat
      541 ttgatgagct aagtccgaga gcccgtgctg gatatccact gtgggtttcg tcacaaaatc
      601 cacagcaccg gcttccaaag cctgtagcgt cgtcgcggct ccttgttcag ttaacgaact
      661 caccatcacc accggcatcg gccgcgcggc cataattttc tgcaaaaagg tgagcccatc
      721 catttttggc atttccacat ctaatgtcag cacatcagga ttcagggcct tcatcttatc
      781 cctggcggca tacgggtcag gagccgttcc aaccacctca atttcaggat cttgcgctaa
      841 aatttccgtc atgacatttc gaattaacgc cgagtcatca acaatcaaca cgcgtatttt
      901 tttctcattc atacggttcc cctctgtgac aaaattattc cctactaaag gagtgaaaag
      961 tcagaagtta aaaaaaacca cccgcagcct tttggtccct gaactctttt ttcccctgtc
     1021 ttgactcctt actgtgcaaa cccttcttcc ttgcacctgc ttcgtcatgc ccgacaggtc
     1081 aactcgggaa accatcttag ccctttttga atggataccc gcaaaccacc tgcggatatg
     1141 acgaaggggg tcttttctct tcacgcctta agcttttcca cttacctctt ccccgaatga
     1201 gactgcaccc ttaacgtcat actctttcct caaaatagcg tgacgtccgc ttgcacagga
     1261 gcatgttgaa gcttcttccg atactgcccc tcctccttcg caatctcact tgccacgata
     1321 cggtcgagtt ttttcatgag cactttcccc gaatcggtaa aatagtaaac cttccgagga
     1381 cagacatctc cgacatccgc tttgataggc gacagaccct cctgctccaa aaaggcaaga
     1441 gcccaagcgg cattttgggc cccaatatca atggtgccat cataaatttt cccaccgccg
     1501 aagaccttcg cttcgagtcg ctcctttttt ccgcctcgct tgaaaatatc gttcatcagc
     1561 atttccatgg caaaactgcc gtaccgtccc gaatccgccc aattatcgtg tccttcagta
     1621 ttggaaggag ccgctaacat gaaatgattc atccctccca cgccggccag tggatctcgc
     1681 aaacacactg agatacagga acccaacacc gtatagatga ctttgggctc ccggctcaca
     1741 aaatattcac cagggagaat gacggccact tcataaggaa agcgggtatc ctgcatgcgg
     1801 cgtatatgtc caaattccat caatcctgtc gatgacataa tttccttacc ctaaggagta
     1861 aaccgtcagg tgcaggagaa acgggttgaa tgttttctcc ttcacttctt accccccctt
     1921 acgccttact attcacgccg accggttttt gataaatggt tgggcgcaga tacgtaaatt
     1981 gatggtcgat ccattgcaaa ctttctgaat gtccgataaa taaatatccc cccggccgga
     2041 gataccggaa aaacttctcc atgagcttgg cttgggtggg acgatcgaaa taaatcatca
     2101 cattccgaca aaaaatcaca tccagttgac tgcgtatggg aaatgtcggg tccattaagt
     2161 ttatccgtcg aaaggccaca agatgcgcga cctgagggcg cacctgcagt ttccccgctt
     2221 gcggaccttt ccctcggaga aaatgtcgtt tcaccagatc attgggaagt tgactaaccc
     2281 gctcttcctc atagataccc gaggaggctt ttgccaacac tcgcgtagaa atatccgagg
     2341 caagaatttt gatgtcccat cgattgatat cggtaatcgc atcagacaag gtcatggcaa
     2401 tcgtataggg ttcctcaccg gaagatgacg cagaagacca aatcctcaag gtcttggaag
     2461 attgcacctc tggaagaacc tggtctcgaa gaaaatcaaa atgcacaggc tcccgataaa
     2521 actcagtctt attcgtcgac accagatcca gcaatttgat caactcctct tccccgcctt
     2581 tcccgcttac gcaatcgtaa taagcctgat aagaagaaag gtgaagatcc cgaagccgtt
     2641 ttcccaaacg agacaccaag agggtttttt ttggctcatc aagtttgata ccaacctgct
     2701 gataaatcag atgcttaaac agttcaaatt ctttatcagt caacattgcc gtcatcatct
     2761 tgtcctttat gttcaattac accaacgtca ccagtgagga agcgggtagt gaagagaagg
     2821 gtatgaacag tcagaacttc cctttcgact taactctcac tccttacccc tcacctttca
     2881 attcgctggg aaccaatcgc acaacatccc atagaccaca tacaccgtca gaaggtttca
     2941 tccagaaaat ggccagaaat tcctctcacc atcgctccaa ccctcgtttc atatcgaagc
     3001 ttccggagtc caacccccag attggtggtt cccaatctta tagtggtcat cgagaggccg
     3061 gtatgcacaa gaagaaccgg tataaataga gacctatatt tctcgacggt tccatcaaac
     3121 cgatctcgcc cgaccagcca ttgttgggtc acatacgcca ccagtcaaag acctatctga
     3181 tcgcaggcaa ccatcatcga atttctgtat tcggtcacat catgtcggtt ggcggcacgg
     3241 attccaaata gcgacagacc ataggcatgg tcattccgac cggaaccaaa tttcataaac
     3301 gattgttgat gtccatattt tctgctggag gatttccatt ccaggggaac aatgaagtga
     3361 aaacaatcgg caaggaaggt aagaaatata ttacctcatg tggcgtaaaa ggatagccta
     3421 ttcacgttac ccttcagcct tacttctttc cctttttctt ttccatttca tcttatcggc
     3481 acacctctga ccgaattaaa ggaccggaga tactttttcg aattatggaa actcaaggtg
     3541 ggaataaaaa aaggcgggga aggttgttaa accctccccg ccttttctca agacgatgag
     3601 tcttaaaatg aaattatttc agtctgacaa caatggctcc cataaagtca cccttcttcc
     3661 aatctttttt ggtcgtctta gggtgtttgt tatggcaatc ggcgtagcta tcaccccgga
     3721 caatatgatg ccacaaccgt ttgttcaaaa atcatgaggc ccaactgatc agactgggac
     3781 ccgtctattt cacaaggaga tctacaatat cattccagtt tttcagtgta ccaatgatcg
     3841 tcggctctcc gagctgaaca ttgaaggtat cctcccgaac catacgcaac gcaatgacca
     3901 gatcattgaa ttccttcaaa ttgttggttt caaatatggt caagaagtcc acgtctgata
     3961 gcccggtaga atggtacaat ttccgtttga ccgtttttaa ataagctacg gtgggaatcg
     4021 tatgttcttt catcagcgcc acacgctcct cattgggaag attccaccat tcggcatttt
     4081 tccgtatggg gatcataatg gcatatgtcg aaggatctcc ttcgtatttc gccgctttca
     4141 gtttttccaa caattctgga aaatccggag cataattcag ccccttggtc attccactga
     4201 tggtgtattc caaatccaaa tgccggccga ggccggtact ccgaagttgg gtcagtaatt
     4261 gctgattggc cttgaggtcg gcggaatgca gccgaaccat aaaatgggag tccgttgtca
     4321 ggccataggt ccaatagcca tccacggtga cctgaccttt gaatgattcc agcagagctt
     4381 tggcctcctc ccctcctttt tgtctatctt ccgccgacac attcccccag tctttatata
     4441 cctcaaacag ggcaaaggta ccaaaaatcg aggcatcggc cgaccaggat ataccagaca
     4501 aaccaaactg cactacagcg aagaacaaaa gggatacaaa tgcgcgcata gatttttctc
     4561 ctattccaat taggtaatta gggcgaaacg tacccaaaat agattttcag aaaccgttta
     4621 aagaggttct ctgatcggtg attaaaacga aattcgattt ctttcagata catcgggaaa
     4681 tggtacttcg aaaggccccg atagtgatac aaaatatgtt tggcataact ccagaaccct
     4741 taaatgccat tgatgtgatt cttcactctg cgacctcgga caaaggtttt ggcatggttg
     4801 atcgtctgat gtttcccata gcgtttcaac gactgatatc cccgaaacgc atccgtgtaa
     4861 tagaccgacc ccttccgggt cttggcttgg atatgccgca tcaattcctc gggactgacc
     4921 gactcgacca ctttggtata gacgcggttt tcccgctcca gaagcccaaa gacgacgctt
     4981 ttgccggcgg cggcccggcc acgtttgccc ttccgtcgtc ccccaaagga ggcttcatcc
     5041 agctcgattt cccttttgag ttttcctccc tccaactcgg ccaaatgata cagggcttcc
     5101 cgcatccgct ggtacactcg ggtgaccact ttgctatcga cagccaagtc tcgactcaat
     5161 cggtaggccg gttggagcag atagaagcct tgcagaatgg tgatctctcg ccgaagccga
     5221 cggagacttt tgctcttgcc acacaactgg catttcacgt agccccgatt ggtgtgaagc
     5281 acctgaaacg agccacaaaa aatacacttg cgcccccgca aaaagttggc cgcacaacta
     5341 aaaaccgagg ttcgtccagt aaccatgcgc cttttctagc acatactggt tatgaaacgc
     5401 cctaattccc tccaattaat gtcatggatt tttaattttt gggtaaggta attgctgaca
     5461 atctttaatg aaccttggat tcaactcgtt agtgcaacga ttgggtgaat gccaaaacat
     5521 acttgattcg gggttttaaa cccaaggctt ttcctcgggc gattattcag tttgttcatg
     5581 acctgctgaa tctcttgctg agtgatggtg gtaaagggcc gctgcttggg gaaatattgc
     5641 cgaatgagcc cattcgtgtt ttcattgagc ccgcgttccc aagccgcata gggatgcgca
     5701 aagaagaagc ggaccttcag cgcctgcacc atgatcgcat gatgtgcgaa ttcttttcca
     5761 ttatctgacg tcaccgtgtg gagcgggagt cccaggggct tcaataggtt tatgacagcc
     5821 gtggcgacgg tgttggcgcc ccgccgtgcg accttggcga tgagactcag tcgagatttg
     5881 cgctctgtta aggagacgag ggcctgttga tgtcctttgc cgatcatggt atctacttcc
     5941 caatcgccga ggcggctccg gcgttcgacc acggcaggcc ggcgctcgat tgaaagccga
     6001 ttcggaatgt gaccccgccg actgtaggta ccataccgct tccggctgtg gcgctgacag
     6061 cgcaggtgcc ggtagagcgt gccccgcgcg cgcttatcgc gatagacatg ttggtagatc
     6121 cattccggac tgattaacgt cccctgctgc gtgcgcaacc agccactgat ctgtaccggg
     6181 ctccactcgg ccttgagcag gacattgacg cagtgccacg tggcagcggc aatacgccgg
     6241 cgaactttgg tgcgtcgccg gctcagcgtg aagcggtggg cttgttgcgg acgatagccg
     6301 cctcgcccct gattccgccg cacttcacgg gttatggtag atttgtgaac accaatcacg
     6361 ccgccaatct cctgatagga gtgtcccatt ttcatcaaag cctggatctg atagcgttgt
     6421 tccagggtca ggtgggtgaa ggatctcata agtgctcctc ttactggccg gtaaaagaag
     6481 ccaggatgct accgcatctg accctttaaa ccgaaccgtt gagttgcgct tacaagttga
     6541 attcaccaac tcacttaaga aaaatttttg gctctaacaa attcgaaggt gagagcactt
     6601 gggacagagt caatgtaaac aaagaaactt gtagaaatgt ttgccataac aagcaacaat
     6661 aaacttttct tactatgaac ttaatgtgaa ggtttggcta aaaaaaagtt gcaattgccc
     6721 caaatgactt tcgtcttgat ccttatggct acaagatact cgctagaaaa ctcataagcc
     6781 ttctggacca ttccggtacc caatcaacca ccccacgggc atctgcatcg aaggagaaaa
     6841 agcaaatccg attcctatcc aggaaaccag tctgccgaag ttcgataagg ctaaacctgg
     6901 gctatgtacc tttactcaag cttccggaag cacccaggct tccggaagct cacaaaaatc
     6961 tttaactaat tactcggagg ccctcaggct tttattgaaa tttgattatt tggcgggaag
     7021 cttaacacta atggccccgc ccagatcgcc ttccttggcc ccttcttttt tgtagccaga
     7081 aacatccatt tcgcccttag gaccaccgtg gcagcccatg caacctttcc catagaacaa
     7141 gggcagcatc acacgcacgt ccccgccggt tgcctcgctc gcgatcttgt ctccctggcg
     7201 cgggtaagcg gaatcaccaa a
//
LOCUS       DATIAL010000112         3124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1095105, whole genome shotgun sequence.
ACCESSION   DATIAL010000112 DATIAL010000000
VERSION     DATIAL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095105"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..576
                     /locus_tag="VLA60_03675"
     CDS             265..576
                     /locus_tag="VLA60_03675"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HSF08487.1"
                     /translation="MKKLYVSQSLIDVESRKELLDQAQIPATIKNQRSAMLGGEVPFV
                     EVFPELWVLKDEDFDRARTLLKDWEQAKPLETTKWICSGCGELHQKEFTTCWKCGLER
                     R"
     gene            787..1845
                     /locus_tag="VLA60_03680"
     CDS             787..1845
                     /locus_tag="VLA60_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08488.1"
                     /translation="MGFALSGQALPHDLTLLPIPLQSGDASLSWPIFGFLTLLISATV
                     APFLWRYGKTRCHIPCKSTSAKNFPWWGWMGVGWTAFAWILAWTRFSWFQEWQPYTFP
                     LLWFGYIVVINALGFSRTGQCLLMNQPKLLLKIFLLSAVFWWMFEYLNQFVQNWHYVG
                     MTEAPSLLTFFWMTIAFSTVLPAVLGTYAYLFSFLQMKAPFENWYATSIFDSPQTGWL
                     MFSLGCFGLVMIGIWPTLLYFLLWVSPLLIMLGIQIIRRVPTLLSPLAKGNWSPVVLS
                     ALAALVCGGFWEMWNALSLVHWEYSIPYVHALPMGEMPILGYAGYLLFGMECLTVATF
                     FLENPLRRGETYLETLTS"
     gene            1911..3065
                     /locus_tag="VLA60_03685"
     CDS             1911..3065
                     /locus_tag="VLA60_03685"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HSF08489.1"
                     /translation="MKKIHSTPGASIHKPSFVPPVIESASTSTVAIIGSFSKGSMTSP
                     QQVRTWEAFEKEYGGLETEALSSLCIKQFFDNEGKAIWVVRIGTGQIKGVSPFLQGLS
                     LLDRIGDFNILFIPQTEQLRDTHASKVMQAAIALVAKHRAMYVLDVPQRDAPRKTVRA
                     LTTWLNQQSGIHHPNVVMYVPHVQVPQSLKKAPLHIPASGAMAGVWGRTDQQRGVWKA
                     PARTEAILHGVQGIEQTLTQPEMGQLTQLGINPIRETSPSQVVAWGARTLSSNREWQY
                     LSVRRLALFLESSIQQGLGWVVDEPNDEPLWAHIRQAIDVFLQTLFRKGAFQGQKAQE
                     AYFVKCGPDTISSADQNAGSVNIMIGFAPIKPAEFIILTIQQKAKSVRQA"
BASE COUNT          762 a          822 c          730 g          810 t
ORIGIN      
        1 tacttcaacc ataaatcaat gccaggcacc ctgaagcaga aatgaaactc ccagcttgct
       61 tggggatcct tgctcaagaa atgattcctc cggacggcct tctagacctc acagggacat
      121 ggtatgttac tgatagtccc attgatatga gagggagagc ttttgtgaac ctctgaatga
      181 ggccaacatg aaggtatgac cactgcggta tcctcacttg agtattgcct cacatccatc
      241 atcgtctagg tgtataggta aaccatgaaa aaactgtacg tttcgcaaag cctcatcgac
      301 gtggaatccc gaaaagaatt gttggatcag gcccagatac cggccacgat taagaatcaa
      361 cgctcagcga tgttgggagg ggaagtgcca tttgttgaag ttttcccgga attatgggtg
      421 ctgaaagacg aggattttga ccgcgcccgg accttactca aagactggga gcaggccaaa
      481 cctcttgaaa caacgaaatg gatctgctcc ggatgcgggg aattgcatca aaaagaattc
      541 accacatgct ggaagtgcgg gctggaaaga cggtaaaaga atccagccgt gtctcaccaa
      601 gactattcca tcttgaccca gattctttac cgttggctca ggcggccggt ctggtttaag
      661 ctgcaccaag ctttcatgtg tgcctccaat atctacacct agaaatctgt tttttacact
      721 cccatgtctt ccccaaaggt aatcaacggc agcattctta tttttttgct tgttgggctg
      781 cctcctgtgg gttttgcctt atctggccaa gctcttccac acgatttgac tctattaccc
      841 attcccctac aatcaggaga cgcctctctt tcctggccga tcttcggatt cctgaccctg
      901 ctcatctccg caacggttgc cccgtttctt tggcgatacg ggaaaacccg gtgccacatc
      961 ccatgcaaat ccacctctgc caaaaatttt ccctggtggg gttggatggg agtcggatgg
     1021 acggctttcg cctggatatt agcctggacg cgattttcct ggtttcaaga atggcaaccc
     1081 tataccttcc cgctcctttg gttcggctat attgtcgtca tcaatgcctt aggtttttca
     1141 cgaaccggcc aatgccttct tatgaatcag cccaaattgc tcttaaaaat ctttctcctc
     1201 agcgccgttt tttggtggat gtttgaatac ctcaatcagt tcgtgcagaa ctggcattac
     1261 gtcgggatga cggaagctcc ttcgcttctc acattctttt ggatgaccat cgcattttca
     1321 actgttctgc cagccgtcct gggtacctat gcgtacctct tttcatttct gcagatgaag
     1381 gcaccgtttg agaattggta tgccacctcc atattcgatt ctccacaaac cggatggctg
     1441 atgttcagcc ttggttgttt cgggcttgtg atgattggga tttggcccac gctgctatat
     1501 tttcttctct gggtgagtcc gctcctgatc atgttaggca tacaaataat acggagggtt
     1561 ccaacccttc tgtccccatt agccaaagga aactggagtc ccgtggttct ttccgctctg
     1621 gcagccctgg tctgtggagg cttttgggaa atgtggaatg ccctcagcct ggtccattgg
     1681 gaatattcga ttccctacgt ccacgcactc ccgatggggg aaatgccgat cctcggttat
     1741 gccggctatt tgctgttcgg gatggaatgt ctgacggtcg caactttttt tctggaaaat
     1801 ccgctccgtc gaggagagac ctatctggaa accctaacaa gctaatgaag gccgtattag
     1861 gctattcctt ctgaaactaa gctccacaga aggagataaa acaatttctt atgaaaaaaa
     1921 ttcactcgac tcctggcgca tctatccaca aaccgtcgtt cgtcccgccc gtgatcgagt
     1981 cggcctccac ctcaaccgtg gccatcatcg gctcattttc caaggggtcg atgacgtctc
     2041 cacaacaggt ccggacatgg gaagcatttg agaaagaata tgggggactt gaaaccgagg
     2101 ccctttcctc cctttgcatc aaacaatttt ttgacaatga aggcaaagcc atttgggtgg
     2161 tacggattgg aacaggacag atcaaaggcg tgtctccctt tctgcaaggc ctttctcttc
     2221 tcgaccgcat aggggatttc aatattttat tcattcctca aaccgaacag ctccgtgata
     2281 cccatgcgag caaagtcatg caagcagcca ttgctcttgt tgccaaacac cgtgcgatgt
     2341 atgtcctgga tgtccctcaa cgggatgccc cccgaaaaac ggtcagggct ctgacaacct
     2401 ggttaaatca gcaatcagga attcaccatc caaatgtggt catgtacgtt ccacatgtcc
     2461 aagtccccca gtcacttaag aaggcacctc tgcacatccc cgccagcgga gccatggctg
     2521 gggtatgggg aaggaccgat caacaacgag gagtatggaa agccccggca agaacggagg
     2581 ccatccttca tggcgttcag ggtatcgagc agacactgac gcaaccggaa atgggacaac
     2641 tcactcaatt agggatcaat ccaatccggg aaacgtcccc gtcacaggtt gtggcgtggg
     2701 gtgcacgaac cctgtcatcc aacagagagt ggcagtatct ttccgtccgg cgtctggcct
     2761 tgtttctgga atcaagcata caacagggat tagggtgggt agtagatgaa ccgaacgacg
     2821 aacccctgtg ggctcatatt cggcaagcaa ttgacgtatt tctccaaaca ctcttccgaa
     2881 agggggcctt tcaagggcaa aaagcccagg aggcctattt tgtcaaatgt ggacccgata
     2941 ccatttcatc cgctgaccag aatgccggga gcgtcaacat catgatcggg ttcgctccaa
     3001 tcaaacccgc tgaattcatt attcttacaa tccaacagaa agccaaatct gtccgccaag
     3061 cgtgatccct attttcattg aaatctcaaa gctcctgctt tttaaagctt cagtgaatgg
     3121 gaaa
//
LOCUS       DATIAL010000113         2330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_342980, whole genome shotgun sequence.
ACCESSION   DATIAL010000113 DATIAL010000000
VERSION     DATIAL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342980"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..744
                     /locus_tag="VLA60_03690"
     CDS             214..744
                     /locus_tag="VLA60_03690"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSF08490.1"
                     /translation="MNQKRKSILAGLAIVGIMGWIILYPPMSQATNIPAPDIVSPTWL
                     NSEPLKLENLRGKVVMVEFWTFGCWNCRNIEPYVKEWHKKYASKGLVVIAVHSPEFKY
                     EYDVEKVKTYIQEHRIPYAVPIDNEFRNWRQYRNRYWPTLYLIDKRGEIQYTKIGEGG
                     YEETERTIQQLLAEPA"
     gene            complement(950..1756)
                     /locus_tag="VLA60_03695"
     CDS             complement(950..1756)
                     /locus_tag="VLA60_03695"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HSF08491.1"
                     /translation="MTLSRSEFHHLTDVAIRAARQGGAILLDYAQKGFQIRKKDQAIN
                     LVTEADLRSEETIIQTIRHAFPEHQILSEEQGLQDIPTNPVKWIIDPLDGTTNFAHGF
                     PMYNVSIGVEYEGGCILGIVYDPTRDELFLGQQGQGATLNGTPIHVSATPKLNEALLV
                     TGFAYDVHTAKDNNLKEFCAFTLRARGIRRTGTAAIDLCYIACGRFDGFWELHLNPWD
                     TAAGKVIVEEAGGTVTNYLGEPYSIYGSTLIATNSCIHQEMIEVLKSCGA"
     gene            complement(1888..>2330)
                     /locus_tag="VLA60_03700"
     CDS             complement(1888..>2330)
                     /locus_tag="VLA60_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HSF08492.1"
                     /translation="LHWGGDRVDMANGMESRPAFLDHHLAEVARHIPPHYRIHQTTEK
                     WILREAMKNILPDVLYRRQKMAFMAPPAHTQSHQRHALQQLIDKYLNPQHLNTVGLFN
                     PRQIETFLSEYRIDTDPASLVQKDALLNHVLGLHMLHHTFIQTA"
BASE COUNT          609 a          539 c          611 g          571 t
ORIGIN      
        1 ttcaggttta tccgtttgta gaaccattcc gttcggtttt ccctggtctc atttcaggct
       61 gacctctgat cctgaatgag aatgagcagg acttgtaaca aatgatcggc tactgagacc
      121 atttcttcac tggatagatt acatacagag agaaggatgg aatctcaaaa gcatccaggt
      181 gaaagacccg ttcatccaca acagaggcga cccatgaacc aaaaacggaa gagcatactg
      241 gctggtttgg ccatcgtagg cataatgggc tggattatcc tttatccacc gatgagtcag
      301 gcgacaaaca tccctgcccc cgacattgtg agtccgacct ggttgaattc tgaaccatta
      361 aaattggaaa acctgcgtgg gaaggtggtg atggtagaat tttggacctt tggatgttgg
      421 aattgccgga acatcgaacc ttatgtcaaa gaatggcaca agaaatacgc ttccaaggga
      481 cttgtcgtga tcgccgtgca ttctccggag ttcaagtatg aatacgatgt ggaaaaagtc
      541 aaaacctata tacaagaaca ccgtattccc tatgccgtgc ccatcgataa tgaattccgg
      601 aattggcgcc agtaccgcaa ccgctattgg ccgacgctgt atctcatcga caaacgaggg
      661 gaaattcaat acaccaagat tggcgaaggt ggctatgagg aaacggaacg aactattcaa
      721 cagctacttg ccgaaccggc ttagagacgt ttgtgaagag tctgcaaaaa ccaatggtag
      781 tgtgaagagt ggccggaagg gattccaatc aaggcatgtc tcgcgtcgat gaggtgagaa
      841 atggacgggg taaaaccgac ccgtcattca aagttcgaaa ggcattccac cttgacgtaa
      901 ctcatcccac gattaatcga ctcacccgct attgaaatgt tatttatgat tatgcaccgc
      961 aactctttaa gacctcaatc atttcctggt ggatacagct gtttgtggca ataagggtgc
     1021 tgccgtagat ggagtagggc tcacccaggt aattcgtgac tgttcctccc gcttcctcca
     1081 caatgacttt tcccgccgcc gtgtcccacg ggttgaggtg cagttcccag aatccgtcaa
     1141 atcttccgca ggcgatataa cacaggtcaa ttgccgccgt gccggtccgg cgtataccgc
     1201 gagcgcgtag ggtgaacgcg caaaattctt tgaggttatt atctttggca gtatgcacat
     1261 cataagcaaa gccggtgacg agcagcgcct cattcaattt cggtgtggcc gacacatgaa
     1321 tcggcgtgcc gttgagcgtg gcaccctggc cctgttgtcc aagaaacaat tcgtcgcgtg
     1381 tgggatcata gacgataccg agtatgcagc cgccctcata ttccactcct attgaaacgt
     1441 tatacatggg aaagccatga gcaaagtttg tggtgccgtc taaaggatca atgatccatt
     1501 tgacaggatt ggtggggata tcctgcaaac cctgttcttc gctgagaatc tggtgttcgg
     1561 gaaatgcgtg ccggatggtc tgaatgatcg tttcttctga tctaaggtcg gcttcggtca
     1621 ccagattaat ggcttgatcc tttttgcgga tctgaaatcc tttttgggca tagtccagaa
     1681 gaatggctcc accctgcctg gcggctcgga tagccacgtc tgtgagatga tgaaactcgg
     1741 atcgggaaag ggtcatgagt ttacacaatt aatccgctca agcatacacg aagtgcagaa
     1801 gaatcccaaa gcgccacgcg aagagatgct tattgggatg gcgaaccatt tacacacgtg
     1861 ggccgtcgga agaaaccacc tcgccaatca ggcagtctgt atgaacgtgt gatgaagcat
     1921 gtgaagtccc agaacatgat tcagcaaggc atccttttgg accagggagg cgggatcggt
     1981 atcgattcga tattccgata gaaatgtctc gatctgccgg gggttgaata acccgacagt
     2041 gttgaggtgt tggggattca ggtatttgtc gattaactgt tggagggcat ggcgttgatg
     2101 agattgggta tgggcgggag gagccatgaa agccattttc tgtcgtcgat acaggacatc
     2161 cggcagaatg ttcttcatgg cctcccgtaa aatccatttt tccgtggtct ggtgaatgcg
     2221 ataatgggga ggaatatgcc gtgcgacctc agctaagtga tgatccaaaa aggcgggacg
     2281 ggattccatg ccattagcca tatccacgcg gtctccgccc caatgtaaaa
//
LOCUS       DATIAL010000114         5429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_343228, whole genome shotgun sequence.
ACCESSION   DATIAL010000114 DATIAL010000000
VERSION     DATIAL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343228"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VLA60_03705"
     CDS             <1..1756
                     /locus_tag="VLA60_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF08493.1"
                     /translation="FTLEAQEGRLFKPLAYTKTFAMFFAALLSIGLAPLLMKWFIRGR
                     IMAERRNPLNRFLVWIYTPLLKGALNARWLVVALAILGMILVVPVYQQLGSEFMPPLN
                     EGTILYMPTSIPGMSIKQASTILQTQDRLLKEFPEVDQVMGKMGRARTATDPAPLHMG
                     ETIVTLKPQEDWRPGMTWDKLIAEMGKKVKLPGMPNIWWMPIQTRTEMLATGIRSSLG
                     IRILGPELEELERIGLHIESLLHTLQGTRSAYAERVTGGYYVDIDVNRTAAARYGLTV
                     GDVQDVIESAIGGKNIAWTVEGRERYPINVRYPRDLRQDVEALKRVLVSTPNGEQIPL
                     AQLSHISTTTGPPSVRDENGSLASMVFVDIAGEDLGTYVHRAKDLVQKHITLPSGYTL
                     QWAGQYQYLERAQEKLKIVIPLTLFLIFLLLYPNFHSVPRCLLVLLSVPLSLIGAIWY
                     LDFLNYPLSIAVWVGLIALAGVAAETGVIMVMFLDEACARRQRENRLHSLADLRDAII
                     EGAVLRVRPKIMTASAIILGLLPIMWSQGTGADVMKRIAAPMIGGMVTATVLTLLVIP
                     VIYFIWRSRSLKSRIQEA"
     gene            complement(1967..2305)
                     /locus_tag="VLA60_03710"
     CDS             complement(1967..2305)
                     /locus_tag="VLA60_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSF08494.1"
                     /translation="MTALGQGRFLFDTNAALYFMGNALADGLPIGSYHISVISEIELL
                     SYPNLSTKEEAAIREFLSKIETIGLRSDVTETAIAFRRQHRMKLPDAIIAASASVLDA
                     VLLTNDSARC"
     gene            complement(2302..2514)
                     /locus_tag="VLA60_03715"
     CDS             complement(2302..2514)
                     /locus_tag="VLA60_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08495.1"
                     /translation="MLNIRRKIVTDEKKRPVAVMIDYEDWEKIEKLLQEQESATSAPS
                     IAQFAGVLRLTEEPLAYQHRFRQEWG"
     gene            2611..2772
                     /locus_tag="VLA60_03720"
     CDS             2611..2772
                     /locus_tag="VLA60_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF08496.1"
                     /translation="MMTASAIILGLLPIIWSQGTGADVMKRIAAPMIGGMVTATALTL
                     LIIPVIYFI"
     gene            2900..3406
                     /locus_tag="VLA60_03725"
     CDS             2900..3406
                     /locus_tag="VLA60_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08497.1"
                     /translation="MIQAFFHVQGTGCRLNGCLLLLLALWTITACATTLQPPQNEPLP
                     PPENATPQVAQLPLDGNRLIGEHRWIAGILKYKEAVKIQPTLAEADYNLGMALYKKGP
                     VSAASSHFIEAADLASGHPAIWNAPPFRKYGTGELSTPTSAPVGHFGHQHWRQASFVC
                     PNIMRDHA"
     gene            3403..5283
                     /locus_tag="VLA60_03730"
     CDS             3403..5283
                     /locus_tag="VLA60_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HSF08498.1"
                     /translation="MSINKDSQDTRMTRRSLLKGAGALGIMAALGRLLPSYALADTTK
                     AKAVYPSELRGKIIDLRIAESPFQIGELTGMAKTINGTLPGPLIRLKEGDNVTLNVTN
                     RLMEDTSIHWHGLLLPPNMDGVPGVSFAGIKPASTFSYRYPIRQSGTYWYHSHSGFQE
                     QSGVYGPLIIDPIEPEPFHYDRDYVVVLSDWTFESPESVFGNLKKQSDYYNFQKRTAP
                     EFFSDVSRMGLGPALQNYLMWDRMRMDPTDFADVTGYTYTYLMNGMAPDQNWTGLFRP
                     GERVRLRFIAATAMTFYDVRLPGLTMTVVQADGQNVQPVVVDEFRIGPAETYDVIVQP
                     TEERPYTIFADTMDRSGYARGTLAPRAGMIALLPDRRPRPLRTMAAMGMGMHMDGMDM
                     ADMNKHDQHGGTHMSPPGMDKSKNYGTEHMHDKHDISDLPPDESRHSMIPGSTPVKHA
                     PGHHGSGNQSVAEYSRQQLDEPGIGLENTGTRVLVYTDLKSLAPYPDQREPGREIELH
                     LTGHMERYMWSFDGKKYSEAKEPIFFRHGERLRFTFVNDTMMEHPLHLHGMWMHLENG
                     AGSFLPRKHTVIVKPAERISVTITADAPGRWAFHCHLLFHMEAGMFRVVEVSGEEPSV
                     RS"
BASE COUNT         1302 a         1491 c         1378 g         1258 t
ORIGIN      
        1 ctttacatta gaagcgcagg aaggccgtct gttcaagccg ctggcctaca ccaaaacatt
       61 cgccatgttt tttgcggcgt tattgtccat cgggttggcc cccctcttga tgaaatggtt
      121 cattcgcggc cggattatgg ctgaacgacg aaacccgctg aatcgttttc tggtatggat
      181 ctatacccct cttctgaaag gtgctctgaa tgcgcgatgg ctggtcgtcg ccttagccat
      241 actcggaatg atcctcgtcg ttcccgtcta ccaacagtta ggctctgaat ttatgccgcc
      301 cctgaacgaa ggcacgattc tgtatatgcc caccagcata ccgggcatgt ccatcaaaca
      361 agcctccacc atcctccaaa cacaagaccg gttattaaaa gaattcccgg aagtggatca
      421 agtgatgggc aaaatggggc gggcgcgcac cgcaaccgat ccggccccct tacatatggg
      481 cgagaccata gtgaccctga agcctcaaga agattggcgg cccggcatga cgtgggacaa
      541 gctcattgcc gaaatgggca aaaaagtgaa attgccgggc atgccgaata tttggtggat
      601 gccgattcaa acgagaacgg aaatgctcgc caccggcatt cgaagcagtt taggcataag
      661 aatcctgggg cccgaattag aagaactgga acgaatcggg ctacacattg aaagcctgct
      721 tcacacactt cagggcacca ggagtgccta cgccgaacga gtcacgggag ggtactatgt
      781 cgatatcgac gtgaaccgca ccgccgctgc ccgttatggc ctcactgttg gggacgtgca
      841 ggatgtcatt gaatccgcta ttggcgggaa aaatattgca tggaccgtgg aaggccgtga
      901 acgctatccg attaatgttc ggtacccacg ggatctgcga caggatgtgg aagcgcttaa
      961 gcgcgttctt gtcagtacgc caaacggcga acagatccct ctcgcccaac tttctcacat
     1021 ctccaccacc acgggaccgc cttccgtgcg agatgaaaat ggatcgttag cgagtatggt
     1081 gtttgtcgat attgcaggcg aggatctcgg cacctatgtt cacagggcca aagacctggt
     1141 tcagaagcac atcacccttc catccgggta taccttacaa tgggccggtc aatatcagta
     1201 tctggagcgg gcacaagaaa aactcaaaat tgtgattccc ctcacgctgt ttctcatatt
     1261 cctcctcctg tatcccaact ttcattccgt tccacgatgt ctccttgtat tgctatcggt
     1321 tccattgtca ttgatcgggg ctatctggta tctggatttc ctgaactatc ctctcagcat
     1381 tgccgtgtgg gtcggactga tcgccttggc aggagtggcc gcggaaaccg gcgtgatcat
     1441 ggtgatgttt ttggacgagg cttgtgcccg ccggcaacgg gagaaccgac tgcactcatt
     1501 ggcggacctt cgtgatgcca tcatcgaagg tgccgtctta cgagtccgcc cgaaaatcat
     1561 gaccgcctcg gccatcatcc tgggtttgct gcccatcatg tggtcccagg gaacgggtgc
     1621 cgatgtgatg aaacgcatcg cggcgccgat gatcggcgga atggtcacgg caacggtgct
     1681 cacactgttg gtcatccccg tcatctattt catctggcga agtagaagtc tcaagagtcg
     1741 tattcaggaa gcatgaacgt tccatctctc agccacattt ttcagccata cgcctcatcg
     1801 agtctgtgta atgtcttgcc cttcgggggt aagcaacgac agataaatgg aggaaaagct
     1861 tcagttccga gtcaaggaag aaatcaaggt gacagggctt catgtttgcg attgtccgat
     1921 ggccttcatc gaagcttgag tgccatcgcc cgaatacccg gcagttttag cagcgcgcgg
     1981 aatcgttcgt caaaagaacg gcatccaata cgctggcaga agcggcaatg atcgcatctg
     2041 gtaatttcat ccggtgttgt cgacggaatg cgatcgcagt ttctgtgaca tcggatcgta
     2101 atccaatggt ctcgatttta gagagaaatt cacgaatggc agcttcttcc ttcgtggaca
     2161 gattgggata agagagcaat tcaatttcag aaatgaccga aatatggtac gaaccgatgg
     2221 gaagaccatc agctaatgca ttgcccataa aatacaatgc ggcattggta tcgaataaaa
     2281 accgcccctg ccccagagct gtcatcccca ctcctggcga aaacgatgtt gataggccag
     2341 cggctcttct gttagtcgaa gcacacccgc aaattgtgct atggatggtg cggatgtggc
     2401 agattcttgc tcttgtaata gtttttcaat tttttcccaa tcttcatagt caatcatcac
     2461 agccaccggc cgcttctttt catccgtgac gatttttcgc ctgatattca gcatatttcc
     2521 tcatttcttt aaaaagatcc ccggatgggt tgtcttgtgc gtttcatggt atcacaggag
     2581 aggattgaac caaaatagat tcgcccgaaa atcatgaccg cctcggccat tatcctgggc
     2641 ttgctcccca tcatatggtc ccagggaacg ggtgccgatg tgatgaaacg catcgcggcg
     2701 ccgatgattg gcggaatggt cacggcaacg gcgctcacgc tgttgatcat ccccgtcatc
     2761 tatttcatct aacggcggcg acaattaaaa ttaaatcatt gaaaatttct gatacgggac
     2821 catggcagca acacaccaaa ttgtaaaagc tccgccattt gtggaatatc tacatcatag
     2881 gagaaaggca gttcaattca tgattcaagc attctttcac gtacaaggga caggttgtcg
     2941 attgaacggg tgtctcctgc ttcttctcgc gctatggaca atcacggcct gtgccaccac
     3001 acttcagccg ccgcaaaacg aacccctccc gccacccgag aatgcgactc cacaggtcgc
     3061 ccaactgccc ctggatggca atcgtctgat tggcgaacac cggtggatcg ctggaatcct
     3121 gaagtacaaa gaggcagtga agattcagcc aacattggcg gaggcggatt acaacctggg
     3181 catggccctc tacaaaaaag gtcctgtttc agcagcgagt tcccatttta tcgaagcggc
     3241 cgatttggct tccgggcatc ccgccatttg gaatgctcca ccgtttagaa aatatggcac
     3301 gggtgaattg agtacaccaa catcggcacc agttggtcat tttggacatc agcattggcg
     3361 ccaagcatct ttcgtatgcc cgaatataat gagagatcac gcatgagcat caacaaggat
     3421 agtcaagaca ctcgaatgac gcggcgctcg ttactgaaag gggccggtgc gctgggaata
     3481 atggcggctt tgggtcgcct ccttccttcc tatgctctgg ccgacacaac caaggcaaaa
     3541 gcggtttatc cttcagaact acgcggaaag atcattgatt tacgcattgc cgaatctcca
     3601 tttcaaatcg gagagttgac cggaatggcc aagaccatta acggcacgtt acccggtcct
     3661 ttaattcggt tgaaggaagg tgacaacgtg acactcaatg tcacgaaccg gttaatggaa
     3721 gatacgtcca ttcactggca tggactactt cttccaccaa acatggatgg cgtgcccggc
     3781 gtcagcttcg ccggcatcaa gccggcctca accttcagct accgctaccc catccggcaa
     3841 agtggaacct attggtacca cagtcattcc ggcttccagg aacaatcggg tgtttacgga
     3901 ccgctcatca tcgatccgat tgagccggag ccgtttcact acgatcggga ttatgttgtg
     3961 gtgctctcgg attggacctt cgagtcgccg gaatcggttt ttggcaatct caaaaagcaa
     4021 agtgattact ataatttcca gaagcgcacc gcccccgagt tcttttccga tgtgtcacga
     4081 atgggattgg ggccggcgtt gcagaactat ctgatgtggg accggatgcg gatggaccct
     4141 acggacttcg cggacgtcac cgggtatacc tatacctatt taatgaatgg aatggcacct
     4201 gaccaaaatt ggaccggatt gtttcggccc ggcgaacggg tgcgattgcg cttcatcgcc
     4261 gccaccgcca tgacattcta tgacgtacgc ttgcccggac tcaccatgac cgtcgttcag
     4321 gcggacgggc aaaatgtgca gcccgtcgtg gtggatgaat ttcgtatcgg cccggcagaa
     4381 acctacgatg tgatcgtcca acccaccgag gagcggccct ataccatttt tgccgacacg
     4441 atggatcgaa gcgggtacgc gcgcggcaca ctggctccgc gcgcaggaat gatcgccctc
     4501 ctccccgacc gccgtccgcg gccactccgg accatggctg ccatgggcat gggcatgcac
     4561 atggacggca tggacatggc cgacatgaac aaacatgatc agcacggcgg gacacacatg
     4621 tcgcctccgg gaatggacaa gtctaaaaat tacgggacgg aacacatgca tgacaagcac
     4681 gacatctcag accttccacc ggatgaatcg cgccattcga tgattcccgg cagcacccct
     4741 gtcaaacacg ctcccggtca tcatggttcc ggcaatcaat ccgtcgccga atattcgcgt
     4801 caacagctgg atgagcctgg catcgggctg gagaataccg gcacacgggt gctggtctac
     4861 accgatttga aaagccttgc gccctatccc gaccaacggg aacctgggcg cgaaattgag
     4921 cttcacctca cgggccacat ggaacgttac atgtggtcat tcgacggcaa aaaatattca
     4981 gaagccaaag agcccatttt ctttcgtcac ggtgaacgcc tgcgcttcac cttcgtcaac
     5041 gatacgatga tggagcatcc cctgcatttg catggtatgt ggatgcacct tgagaacggg
     5101 gcgggttcct tcctgccgcg caaacacacc gtcatcgtga aaccggccga gcggatctcc
     5161 gtcaccatca cggctgacgc tcccggacgt tgggcctttc attgccatct acttttccat
     5221 atggaagcgg gcatgttccg cgtcgttgag gtttccggcg aggaaccttc ggtgcgttca
     5281 tgaggacaat ttttcgatgc atcctctcac tcacagttat cagtcttttt atttgggatt
     5341 tcaattcgtc aggtatcttc gcccaatcca tccccgacga atcgacgtcc acccatcaac
     5401 acacacacca cccccgaacg atacagccg
//
LOCUS       DATIAL010000115         5553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1310936, whole genome shotgun sequence.
ACCESSION   DATIAL010000115 DATIAL010000000
VERSION     DATIAL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310936"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VLA60_03735"
     CDS             <1..871
                     /locus_tag="VLA60_03735"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HSF08499.1"
                     /translation="ARAPVMNINAQTQLCGLLGNPVDHSLSPAIHNAAFRQLGLNFVY
                     LAFPVQDLEGAVRGLRALGHIRGLSVTIPHKVTILPLLDSVETTANHIGSVNTIVKDR
                     GLLVGSNTDASGALQALQQGGVERTAQRVVILGSGGAARSIAFALGVEGKIEHLTILG
                     VDNQERTTLANDLKAKTSIRLHDNSLTPETLQSALAQAQLLIHCTPIGMHPKIDESCV
                     PKHLLHRDLTVMDIVYNPLNTRLLQDAQAAGCRTIQGINMFLYQAVGQFELWTGKSAP
                     IEVMRHVLTSHFS"
     gene            1119..1625
                     /locus_tag="VLA60_03740"
     CDS             1119..1625
                     /locus_tag="VLA60_03740"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HSF08500.1"
                     /translation="MNIVFIGYRGTGKSTVATILGQRLGRRVISTDEEIVKETKQSIP
                     QLIEQFGWDHFRGLETQMCQKLTSQDNLVIDTGGGLILKEENVRMLKENGKIFWLTAE
                     VPTIASRISGDTQRPSLSGTKSFVEEIEEILEIRKPHYQAAADHVIPTDQISPEQIAD
                     TILSRLSI"
     gene            2624..3121
                     /locus_tag="VLA60_03745"
     CDS             2624..3121
                     /locus_tag="VLA60_03745"
                     /inference="COORDINATES: protein motif:HMM:NF020254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HSF08501.1"
                     /translation="MNPSTHNQMNPLADHWQKPWVIEWSQYLLDSYAYWLKKELIPRQ
                     GTSLEQAERLFTCPFVVVSHGLEDDPIFNYGNRTASTLWEMDWDQLTQTPSRQTAEPV
                     NREKRARMLHQAKTKGFIADYRGVRISRSGKRFVVERATVWNIRTPDGTPLGQAATFS
                     TWTFI"
     gene            3277..4338
                     /gene="glk"
                     /locus_tag="VLA60_03750"
     CDS             3277..4338
                     /gene="glk"
                     /locus_tag="VLA60_03750"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HSF08502.1"
                     /translation="MILAADIGGTKINLALFDWNKERVDPIREDTVWTADYESFEEVL
                     TEFLEEPTSGDSESGEAPGDSEALSSESTPPSPLGPLTAACFGVPGPVLNNTCRPTNI
                     PWTIEGDKLAEFLNIPHVRLLNDLEATAYGLRLLQPDEVEDLNPNAPSPPPDGTRVLL
                     AAGTGLGESLLIWTGEDYQICPSEGGHADFAPNSDLEIDLLRYLRTSYLHVSYERVLS
                     GPGLHSIYQFLRDTKKNEPTWFAEKLPTGDPAALITEAGLTGKPEICKEALQLFISIY
                     GAEAGNMALKALALGGVYLGGGIAPKIISALREDTFMKAFLAKGRYKRLLAKIPVRVI
                     LNPHTALLGAASVAAGMAG"
     gene            4571..5272
                     /gene="rpiA"
                     /locus_tag="VLA60_03755"
     CDS             4571..5272
                     /gene="rpiA"
                     /locus_tag="VLA60_03755"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HSF08503.1"
                     /translation="MPSPSTPLTPDQQKAQAARKALEFIQDGQILGLGTGSTVHYFLT
                     ALGQRVQQGLRVRGVPTSQTTAVYARQLGIPLLNNDDPWDIDVAVDGADQVDPQLNLI
                     KGGGGALMREKIVAKAAQQFIVIVDQAKQVDRLGLPFPLPVEIVPFGWRTTQRHLENL
                     GWPSPLRHRAGQPFLTDNGNYVADLHIPEITDPSALELSLNQIPGVVECGLFLMMTSL
                     LITGTNDGPVLTRAS"
BASE COUNT         1373 a         1525 c         1372 g         1283 t
ORIGIN      
        1 tgcgagagca cccgttatga acattaatgc acaaacccaa ctctgcggcc tgttaggcaa
       61 cccggtcgat cattcgcttt ctcccgctat tcacaatgcg gcctttcgtc aattgggatt
      121 aaattttgtc tatctggcat ttcccgtcca ggatctcgaa ggcgcagttc ggggcctccg
      181 ggctttgggc catattcggg gactcagcgt gaccatccca cataaagtca ccattctccc
      241 tttgttagat tccgtcgaaa cgaccgccaa ccacattggc tcggtgaata ccattgtcaa
      301 agaccggggc ctgttagtgg gctccaacac ggatgcatcg ggcgcgctcc aagccttgca
      361 acaaggtgga gtggaacgca cggcacaacg ggtcgtcatt ctaggttccg ggggagccgc
      421 tcgctccatt gccttcgcac tcggcgtgga aggcaaaatt gaacatctga ccatcctagg
      481 cgtcgataat caggaacgaa caaccttggc gaacgacctc aaagccaaga catccatccg
      541 cttgcacgac aactccttaa ctcctgaaac actgcaatct gcattggcac aggcccaatt
      601 gcttattcat tgcacaccca ttggcatgca cccaaagatc gacgaaagct gtgtccccaa
      661 gcatctgttg catcgcgatc tgacggtcat ggatattgtc tacaacccac tcaatacacg
      721 tttattgcag gacgcccagg cggcaggatg ccgcaccatc caagggatca acatgtttct
      781 ctatcaggct gtgggacaat ttgaactctg gaccggcaaa tccgcaccaa tcgaggtgat
      841 gcgtcacgtg cttacctccc acttctcttg aataaaggat atcgggctcg gatattgttc
      901 ccagcctcaa cgagagtctt tgcgatgaag aattccatag gagaaaggag tggccgtggt
      961 gacattcctc caaatgtgtc acctgcccgc tggcctgaac aaaattctcc ctggttcccc
     1021 tccggtattc ccaacggttt ttacaagaat tttctctccg gttctgttcc aacctgtccg
     1081 aaaagggttt ctaaccaaca tttcaaaaca ggagatttat gaatattgtc ttcataggat
     1141 accgaggaac ggggaaaagc actgtggcaa caatcctggg tcaacgtctc ggacgaagag
     1201 tcatctccac tgatgaggaa atcgtcaagg agaccaagca atccattcct cagcttatcg
     1261 agcaattcgg gtgggatcat ttccgcgggc tggaaacgca gatgtgtcaa aagctcacca
     1321 gccaggacaa tctggttatc gacacgggcg ggggactcat ccttaaggaa gaaaacgtca
     1381 gaatgcttaa agagaatgga aagattttct ggctgactgc cgaagttccg actattgcaa
     1441 gtcgaatttc aggtgatacc caacggccct cgctgtcagg aaccaaatcc tttgtggaag
     1501 aaatcgaaga aattctcgaa attcgtaaac ctcattacca agccgccgcc gatcatgtca
     1561 ttccaaccga ccaaatttcc ccggaacaaa ttgccgatac cattctttcc cgcctttcga
     1621 tctagaacgt tcactcagga aactggacag cagggtcggt cagtacaaaa aaaagagaga
     1681 gcgtaagaaa ctccgctgtg cttacatacc gtctgtcttt tctccggtgg cggctgtacg
     1741 gcttcgccac gccacagacc aggttccctt tccaccctcg ccccagtgga ggaaaaggag
     1801 agccaaacag ttaagaattg ggtaataaag cagtctaatg cagttaacca actatggtat
     1861 ccatgtcgtt tttggagatg ccgggtatcc cccggcagcc gagccacttt tgtttcggca
     1921 aaagtggcca aaaccattga cgcccagaca ggcttcattg gatgggacag acgcgagcct
     1981 tataagagca gaccaactcg ctgcgctcag acaaggtctg ctgaatgccc agagcgtccg
     2041 tcccttgagc ctgtcagcag gcgtcggatg aggcaaagtt ggagaggtaa ggtggggttt
     2101 ttacatacca cgttccaaag cagcacgagc ggtttttgtg atgccgagat tcgtcctaga
     2161 agatcagagc atcattggaa tcccgtggcc ttgtggcgag gccaagtcgt tttgccgact
     2221 tcggaggaag gcgcgcattg cttgagcgca gcgagtttgc gcgccgccgg agtcggcgac
     2281 acggcgcagg gcacccgaag ggccacgcca cggccaccat ggttttgggt ccttttgccg
     2341 aaacaaaagg acctcgtccg ccgggcgaaa tccggcacaa aaagatacca agaaagcgcc
     2401 aattaatttt ggatacaagc catcctggcc cagggaaaca gacatgggat ccaccacggc
     2461 cggaccagct cagatgatct tgaattgaaa gatgcatgat ttcaagacct gaccaggaat
     2521 ccgcttttcg ctgttttgga aaattagtaa tgcttggtcc ttttcaacga atccaaaaga
     2581 gcattacact gcttctgatt cgacgctccg catcatcacc cggatgaatc cctccactca
     2641 taatcagatg aatccgttag ctgatcattg gcaaaaaccc tgggttatcg aatggtccca
     2701 atatttattg gacagttatg cctattggct gaaaaaagaa ctgattcccc gtcagggtac
     2761 ttcactggaa caggcggaac gcttgtttac ctgcccattt gtggtcgtct ctcacggatt
     2821 ggaagatgac ccaatcttca attatggaaa ccggactgcg tcaaccctgt gggaaatgga
     2881 ttgggaccaa ctgactcaaa cgccctcccg gcaaacggcc gaacctgtga atcgtgaaaa
     2941 acgggcccgc atgctgcatc aggctaaaac caaggggttc attgccgatt atcgcggagt
     3001 ccggatctcc cgatccggaa aacggtttgt agtggaacgc gccacggtgt ggaacattcg
     3061 cacaccggat ggaaccccgc tcggacaggc ggccaccttt tcaacctgga cgttcatata
     3121 agtcgtcgaa cgtctcctcc ttaccgcctt tccctgtgat ccagatctgc caagagaggc
     3181 tctcccggcc ctcgcttgcc ctgaggattt tacctgggct atggtgccgt ggggttgata
     3241 attgacgctt caaccatttt tcatggggaa cgtaacatga ttttagcggc tgacatcggt
     3301 ggaacaaaaa tcaatctggc cttgtttgat tggaacaagg aacgggtaga tcctattcgg
     3361 gaggatacgg tttggaccgc tgattacgag tctttcgaag aagtgttgac ggaatttctc
     3421 gaagaaccga cttctggcga ttccgaatcg ggagaagccc cgggcgacag cgaggccctt
     3481 tcgtccgaat ccactccccc ttctccactg ggacctctga ccgccgcctg ttttggcgtg
     3541 ccgggaccag tcttgaacaa tacctgccga cccacaaata ttccttggac aattgaagga
     3601 gacaaactcg cggagtttct gaatattccc catgttcgtt tgttaaacga tttggaggcc
     3661 actgcctacg ggctacggct ccttcaaccg gatgaagtag aggacctcaa ccccaacgcc
     3721 ccttctcccc cacctgatgg cacgcgggtc ttattggccg cgggaaccgg cctgggagaa
     3781 tcgctgctca tctggacagg ggaggactac cagatctgtc cgtccgaggg aggacatgcc
     3841 gattttgcac ccaacagcga tttggaaatc gatttgctgc gatatttgcg aaccagctat
     3901 ttacatgtca gttacgaacg ggttctttcc ggcccaggcc tccacagcat ttatcaattt
     3961 ctccgggaca ccaagaaaaa cgagcccacc tggtttgccg aaaagctccc caccggtgat
     4021 ccggcagcat tgattacgga agccggcctg acggggaaac cagagatttg taaagaagcc
     4081 ctgcagctct tcatctctat ttacggggca gaagccggca atatggccct caaggccctg
     4141 gccctgggtg gcgtctatct tggcgggggc atcgccccaa aaatcatatc cgcgttacgg
     4201 gaagacacat tcatgaaagc ttttctggca aagggccgct acaaacggtt actggccaag
     4261 attcctgttc gggtgatttt gaatccccat accgcattgc tcggggcggc ctccgtcgct
     4321 gcaggcatgg ccggctaaca ccatgaaatc aaagcatacc ctgggtattc ctcagggctc
     4381 agaccccaca tcctcccttc gatatcatac tgacagacct ttgactctgt ttttttcacc
     4441 ctcgtcccgg ccgttttgcc cgataacact cgcagagcac caggctttga gaaagaatgt
     4501 ctgctagttt cgatgacacc aagcctccgg ccgtttacca acgcataatt cccgagcgtg
     4561 agacctttct atgccctccc catcgacgcc attgacaccg gatcagcaaa aagcccaggc
     4621 cgcccgcaaa gcccttgaat ttatacaaga tggccagatc ctgggactcg gtaccggttc
     4681 cacggtccat tattttttga cggccctagg ccagcgcgta caacaaggat tacgggtccg
     4741 tggcgtgccg acttcacaaa ccacagcggt ttatgccaga caattgggca ttcctttact
     4801 gaacaatgat gacccctggg acattgatgt ggcagtggat ggcgccgatc aggtcgaccc
     4861 ccaactgaat ctgattaaag gaggaggggg agcgttaatg cgggaaaaga ttgtggcgaa
     4921 agctgctcag caattcattg tcattgtgga tcaggccaaa caggtggacc gtcttgggct
     4981 tcctttcccc ttacccgttg aaatcgtgcc attcggttgg cgaacgacac agcgacatct
     5041 tgagaatctg ggttggcctt ccccattgcg ccatagagcc ggccagcctt ttctcacaga
     5101 caatggaaat tatgtggcgg atctccatat tcctgaaatt accgacccct ccgcactcga
     5161 attgtcgctg aatcaaattc cgggcgtggt ggaatgcgga ctcttcctga tgatgacatc
     5221 cctattgatt accggcacaa atgatggtcc cgtgctcacc cgcgcatctt gagtcatgcg
     5281 tcttctctgg taaaattaac cgatgattgt ttgccgttgc atgctaccgc ccttacattc
     5341 aaggttgata aaatagggaa catgcctaag gatcaagaaa tcaggggtct gataacagga
     5401 ctcccctaac caaagaagaa gatatcctgc atgaggagac gatatctatt gcgaacatcc
     5461 tctttaaaaa acagacatta tttctggagg gttgcgatcg cggtgggcct cctgggattc
     5521 acccatattc aatgctctca ggcaccatcc tcc
//
LOCUS       DATIAL010000116         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_450613, whole genome shotgun sequence.
ACCESSION   DATIAL010000116 DATIAL010000000
VERSION     DATIAL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450613"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1026
                     /locus_tag="VLA60_03760"
     CDS             166..1026
                     /locus_tag="VLA60_03760"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSF08504.1"
                     /translation="MSRTLDPSLAGTPQRDYPTMFLFGLIVVATLVGLPVYAYFYDFS
                     WVDWTMFIVLYMTTGLGITVGYHRLITHRSFKCPDWVKAAFLIAGGMALENSALRWAG
                     DHIRHHARCDQSEDPYNATLGFWHSHCGWIFWKDPNRDPKYATRLLQDPLIMWQNQYY
                     LPIVLSGLFLPFIVGLLYNGWIGGIGCFLLAGLARTFFVLNSTFFINSICHIWGDQPH
                     GTSDSSRDSWWISLLTFGEGYHNYHHMYQSDYRNGVRWYNFDPSKWLIWILYKLGLAY
                     DLRRQSHNQL"
     gene            1222..2391
                     /locus_tag="VLA60_03765"
     CDS             1222..2391
                     /locus_tag="VLA60_03765"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002874.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HSF08505.1"
                     /translation="MTNRYVMNTYTRLPISLVRGNGCLVYDVEGREYLDCLAGIAVNI
                     LGHAHPDLVETITRQARHLIHTSNLFYTEPQARLAQRLVELSFADKVFFCNSGTEANE
                     AAIKLARRYAHNTFGPERFEILTMVNSFHGRTLASLSATGQPKLQEGFDPLVPGFRYI
                     PFDDLETLKANITPQTAAVMVEPIQAEGGVRVPHPEYLKGLREFCTERQILLIFDEIQ
                     TGMGRTGTLFAYQQFGIQPDIMTLAKGLGGGIPIGACLATDTVSSAFEPGTHASTFGG
                     NPLACATALKVLDILIDGGKLEQSRVAGNYLAKGLGSLKEQFSCIREARGMGLLQGLE
                     LSVDGKPLVLKCLERRVLINCTMGQILRFVPPLIISMTQIDRLLSVLSDVFSKQR"
     gene            2395..3387
                     /gene="argF"
                     /locus_tag="VLA60_03770"
     CDS             2395..3387
                     /gene="argF"
                     /locus_tag="VLA60_03770"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSF08506.1"
                     /translation="MPSFDQSQRTHKSSSVAQKNPPLLPKDLLTVADIPRQTIHHLLT
                     LAQTLKASRGKGRAVRPLKGKTLGLIFEKPSTRTRVSFEAGMNQLGGQALFLASEKIQ
                     LSRGESLADTAKVLTRYLDGLVVRTFEQASLEEWARHTSIPIINGLTDHCHPCQALAD
                     LLTIVEKKGQAKGLKLAYIGDGNNITHSLLEAGAKVGMHVAVGCPEGYEPDPVIVEAA
                     KEEGLQTGARIEITHDPYIAAQDADVIYTDVWISMGQEHEQQPRMSALAPYQVNSQLM
                     QQAKPHALVMHCLPAHRGEEITEDVLDGPQSVILDQAENRLHIQKAILLTWIGK"
     gene            3393..>3919
                     /locus_tag="VLA60_03775"
     CDS             3393..>3919
                     /locus_tag="VLA60_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HSF08507.1"
                     /translation="MKRRAGSDPSTTSTRSTVPIRSTKPKVILAYSGGLDTSVILHWL
                     KVEHGCDVIAFCADLGQGEDLSAIRKKALQVGAIKAYVEDLREEFVSDYVFPMLRGNA
                     VYESSYLLGTSIARPLIARRQVELAKKERAIYVSHGATGKGNDQVRFELAFAALAPHL
                     KVIAPWREWSMGSRQ"
BASE COUNT         1009 a          952 c          925 g         1033 t
ORIGIN      
        1 aagggcaggt catgacctgt attcttcggt gcaaccaacc cacttgaatg taaaagagat
       61 accaattaca ggtttcttta attaactcat ccctcaccgt tgggcttcct tttttggatt
      121 aatctcccaa ttcggtgttg gagatatgtg aggacttaga ctgctatgtc acgaacccta
      181 gatccctctt tggcggggac cccacaacgg gattacccca cgatgtttct gtttggtctt
      241 attgttgtgg ccactctcgt cgggctcccg gtgtatgcct atttctacga tttttcctgg
      301 gtcgactgga ccatgtttat cgtattgtac atgaccacag gactaggtat tacagtaggc
      361 taccatcgat taatcaccca tcgtagtttt aaatgccctg attgggtcaa agccgctttc
      421 ctaatcgctg gaggtatggc actagaaaat tcggcgttac gatgggcagg tgatcatatc
      481 aggcaccatg cccgttgcga ccaaagcgaa gatccttata acgccacgct gggattttgg
      541 cacagccatt gtggctggat tttctggaaa gatcccaatc gtgatcccaa atatgccact
      601 cgcctgctac aagatccttt aattatgtgg caaaaccaat attacctccc catagtcctg
      661 tcaggacttt tcctgccttt catagtggga ctcttgtaca atgggtggat tgggggtatt
      721 ggatgttttc tcttggccgg gctcgcgcga acgttctttg ttttaaattc aactttcttt
      781 atcaactcta tttgtcatat ttggggtgat caaccccatg gaacatccga ttccagccga
      841 gatagttggt ggatttcttt actcaccttc ggagagggtt atcacaatta tcaccacatg
      901 taccaaagcg actatcggaa cggggtgcgg tggtataatt tcgatccatc caaatggctt
      961 atttggatat tatacaaatt gggattagcc tacgatcttc ggcgtcaatc tcataaccaa
     1021 ttgtagccac tgatcaactc agacaacact cctggtctga acctggattg aacagtccaa
     1081 ggttctcctg ttgtatttca ctatcgatca ataacgcctc catctcagct tacccatcat
     1141 tgcggacttg agaaacgtgt gataaattcg cctgtaatca tgcgggtttt ggaaaatatg
     1201 ctcatcaacg aaatccaaga aatgacaaat aggtatgtga tgaataccta tacccgcctc
     1261 cccatttcgc ttgtccgagg aaatggatgt ttggtttatg atgtagaagg tcgggaatat
     1321 ctcgattgtc tcgctgggat tgccgtcaat atcctgggcc atgctcatcc ggatcttgta
     1381 gaaactatca cacgacaagc ccgacacctt atccatactt ccaatctgtt ttatactgaa
     1441 ccccaagcca ggttggccca acgcctggta gaactatcct ttgctgacaa agtgttcttt
     1501 tgcaatagcg gtacggaggc caatgaagca gccatcaagt tagccaggcg gtatgctcac
     1561 aacacttttg gtcctgaacg atttgaaatt ctgaccatgg tgaattcatt tcatggacga
     1621 acattggcaa gtctttcagc aacgggccaa ccgaaattac aagaaggatt tgatccactt
     1681 gttcctggat ttcgatacat tcccttcgat gatctggaga cgttgaaagc caatattacc
     1741 ccacaaaccg cagccgtgat ggtagaacct attcaagcgg aaggtggggt ccgagttcct
     1801 catcctgaat acctgaaagg cttgcgagag ttctgcaccg aacgacaaat tctcctgatt
     1861 ttcgatgaga tccaaacggg gatgggacgc acgggaacat tgtttgccta tcagcagttt
     1921 ggcattcaac ctgacatcat gacgctggcc aaagggctgg gaggtggaat ccccatcggg
     1981 gcctgcctgg ctacggacac cgtctcttcc gcatttgaac caggaactca cgcatcgact
     2041 tttggcggga atcctctagc ttgtgcgact gccttaaaag tattggatat tcttattgat
     2101 ggtggaaaac ttgaacagag ccgggttgcc ggtaactatt tagccaaagg gcttggttct
     2161 ttgaaagaac aattctcctg cattcgtgaa gcacggggca tgggcttatt acagggattg
     2221 gagttatccg tagacggaaa accactcgtt ctcaaatgtc ttgaacgccg agttctgata
     2281 aattgcacca tgggacagat actccgtttt gtaccgccgt taattattag catgactcag
     2341 attgaccgac tcttatccgt cctctctgac gttttttcca aacagcgata agtcatgcca
     2401 tcatttgatc agtcccaacg cacccataaa tcctcttctg tggcacaaaa gaatccgccg
     2461 ttgcttccta aagacttgtt gactgtggcc gatattccac ggcaaacaat ccaccatctg
     2521 ctgaccctcg ctcaaacctt aaaggcttct cgaggaaaag gccgtgctgt caggccgttg
     2581 aagggcaaaa ctcttggact catttttgag aagccctcga cgcgcaccag agtgtctttt
     2641 gaagccggca tgaatcaact gggcggacag gccttatttt tggcgtcgga aaaaatccaa
     2701 ttgagccgtg gtgagagcct ggccgatacg gcaaaggttt taacccggta cttggatggc
     2761 ctcgtcgtga ggacctttga gcaagcttct ttagaagaat gggcaaggca tacctccatt
     2821 cccatcatca atgggctaac ggaccattgc catccatgcc aggccttagc ggatctattg
     2881 acgattgtag aaaaaaaagg gcaagcgaag ggtttaaagc tggcctacat tggagatggg
     2941 aacaatataa cccattcgct tctggaagca ggggccaaag tgggaatgca tgttgccgtc
     3001 ggatgcccag aaggctatga acccgatccc gttattgttg aggcagccaa agaggaaggc
     3061 ttgcaaacag gggccaggat tgaaatcacc cacgatccct acatagcagc tcaagatgct
     3121 gacgtgatct acacagatgt ctggatcagc atgggacaag aacatgagca acaacctcgc
     3181 atgtcagctt tagctcctta tcaggtcaac agtcagctta tgcaacaggc aaaacctcat
     3241 gcactcgtca tgcattgcct acccgcccat cggggggaag aaatcaccga agatgtgctg
     3301 gatggtcctc aatcggtgat attagatcaa gcagaaaacc gtctccacat ccaaaaagct
     3361 atcttgctta catggatagg aaagtaaggg ttatgaaaag acgcgccggt tcagaccctt
     3421 ccaccacttc gactcggtca accgtaccca ttcgatcaac aaaaccaaaa gtgatcctgg
     3481 catattccgg tggattggat acttcagtca tcctgcattg gctcaaagtg gagcatgggt
     3541 gtgatgtgat agccttttgt gcagatttgg ggcaaggcga agacttgtcg gctattcgaa
     3601 aaaaggcctt acaagtcggc gcaatcaaag cctatgtaga agatcttcgg gaagaatttg
     3661 tttctgatta tgttttccct atgcttcgcg ggaatgcggt atacgagagt tcttaccttt
     3721 taggaacatc catcgcccgc ccccttattg ccagacgcca ggtggaactg gccaaaaaag
     3781 aaagggcgat atacgtcagc cacggtgcca ccggcaaagg aaatgatcag gtccgttttg
     3841 agcttgcctt tgcggctctg gctcctcacc tcaaagtgat tgctccgtgg cgagagtggt
     3901 ccatggggtc acgacagga
//
LOCUS       DATIAL010000117         2891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1418531, whole genome shotgun sequence.
ACCESSION   DATIAL010000117 DATIAL010000000
VERSION     DATIAL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418531"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            837..1955
                     /locus_tag="VLA60_03780"
     CDS             837..1955
                     /locus_tag="VLA60_03780"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HSF08508.1"
                     /translation="MAGIIKTPFHQRIVIGVEIEAYSINVTDHKIGRRLSKPRPGLSE
                     SGERFTRDASIGSEYNSRPFTTIRESLFLLKAGLRKYLRGLYRSREDDQDYRVPLFVG
                     GWTNRFAGTHLHISVANRKLTKQEATALSWHLHDQLPLLIATGANSPIWDKKVTGKAS
                     NRFLRGNTTYFTPTKRGDLTSVDTRELVFSKGRKTKPPTLEIRVFDSNIPEFVVANLC
                     LVKAVCLRWLRGEAAANRMSHADYLLARTEAATKGMKARLPWKREWMPAPDYLDQFLW
                     EHREEFDAMDIPEEIYEVLRLLKRRYNGARLIHDAVALAIREHPQTWQRRFAKRYRSG
                     LSHLLSGNTLQDFAAELKVPFPSTERVWLGRKRASIDE"
     gene            1948..2694
                     /locus_tag="VLA60_03785"
     CDS             1948..2694
                     /locus_tag="VLA60_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSF08509.1"
                     /translation="MSKRFPAPLIGITCEAVSKRKDFANYDLLCDHRYAAAVTEAGGH
                     PVLLPINHRETLRNRYLEGIDGLVIVGGDDLDPTWYGERPQKRTKVAFQNRSKFEAWL
                     YKAGKTRRLPILGICYGMQLINVLEGGTLHQHIHPPKKGYNVDHEGKKNGLHPVKVIP
                     GTRLAKILGKGRKVVATQHHQGVRTLAPDFIPAAIASDGLIEAMEHPKYPHIFTVQWH
                     PERQLRSQTTQRLLRAFVRSCQHYQRTRHP"
BASE COUNT          758 a          838 c          698 g          597 t
ORIGIN      
        1 acagtccggg aaacgaacca gagaagacct acatgatctt gcttccgata ccttggatca
       61 agccgaagac tggctagata ataccaaaga aacagttgac gattttgtca aaagaggaaa
      121 agcggccgtc atgggcgcct aattccggca cttgttgtac tcgagtggtc agacgatgtt
      181 caaggtattt aaccatgaac gaagcgaaca cgccttggga ggctatgtca gcctgagcga
      241 ccttgtcatc tagagctgtt cgacaagcct gccttcaggg cagtcgaaga ggctcaaccc
      301 aagttcaccg gagtctctca gtcaggtgtt gcctatccta acttgtcgca ggaccttccc
      361 acggggtcag catgaccaaa ataggagata acctcgctaa aggcgcaggt gtttgtcccc
      421 tgcctgaata aggttgtccc ttgttgcaca cgtactcccg gacctccttc aaaccctggg
      481 gcctcgagcc ccaggaacaa tctgaaaaaa tcgaggaaca atttaaccct tctagactct
      541 tcctgcgcag agggatacaa ttagctccta gttgtctgtc ccattgacct tccaagattc
      601 cggtacctat tgaacgtgtt tccacattac aagttattcc cgaaattttg agcggaatac
      661 tctgcagtgc cctgccgtac attcaccacg taccacccct tcccattcgc aagcaatttc
      721 ccttctctga cggatggttc cgccaaggga aggtggcacg aatccgataa gacaccggac
      781 acaaaaccac tcccgttctt ggaccaccac aacagataag gacatacgat cttgccatgg
      841 caggcataat aaaaactccg tttcatcaac gaatcgtcat tggcgtcgaa atcgaggcgt
      901 acagcatcaa tgtcaccgat cacaagatcg gcaggcgcct gtcaaaaccc cggcctggtc
      961 tttcggagtc cggcgaacga tttacccggg atgcctccat cggcagtgaa tataatagcc
     1021 gaccattcac gaccatccgg gaaagcttat ttttactcaa ggccgggttg cgaaaatacc
     1081 tgcgaggcct gtatcgcagc cgagaagacg atcaggacta tcgtgtgccg ttatttgtgg
     1141 gaggctggac gaaccggttt gccggcaccc acctccacat ctctgtcgcc aatcgcaaac
     1201 tcaccaaaca agaagccacc gcgctcagct ggcatctcca tgaccagctc ccgttgctca
     1261 tcgcgaccgg agctaattct cccatatggg acaaaaaagt cacgggcaaa gcctccaacc
     1321 ggtttcttcg tggaaatacc acctacttca cccccacgaa acgaggcgat ctcacatcgg
     1381 tcgataccag ggaattggtt tttagcaaag gtcgaaaaac caaacctccc actctggaaa
     1441 tccgggtctt cgattccaac attcccgaat tcgtcgtggc caatctatgt ctggtgaaag
     1501 cggtctgcct gcgatggctc cggggagaag ccgccgccaa ccggatgagc catgccgatt
     1561 acctcctcgc ccggacagaa gccgccacga aaggcatgaa agccagactt ccctggaaac
     1621 gcgaatggat gccggccccg gactatttag accagttctt atgggaacac cgggaagaat
     1681 tcgacgccat ggatattcct gaagaaattt acgaggtcct gcgcctactc aaacgccgat
     1741 ataacggagc ccggctcatc catgatgcgg tggccctggc cattcgcgag cacccgcaaa
     1801 cgtggcaacg ccgtttcgcc aaacgctatc gctcaggatt aagtcatttg ctcagtggca
     1861 ataccttgca ggattttgcc gcggaactaa aagtaccctt tcccagcacc gaacgggtgt
     1921 ggctgggccg gaaacgagcc tccatcgatg agtaaacgtt ttcccgcccc gttaatcggg
     1981 atcacctgtg aagcggtgtc taaacgcaag gacttcgcca actacgactt gttatgtgat
     2041 caccgctatg cggccgccgt caccgaagcg ggcgggcatc ccgtattgct cccgattaac
     2101 catagggaaa cccttcgcaa ccgctacctg gaaggcatcg acgggttggt cattgtcgga
     2161 ggagacgatc tggatcccac ctggtatgga gagcgccccc aaaaacgcac aaaagttgcg
     2221 tttcaaaacc ggtccaaatt tgaagcctgg ttatacaagg ccgggaaaac ccgtcggttg
     2281 cccattttag gaatctgcta tggcatgcag ctaatcaatg tcctggaagg cggaactctt
     2341 catcaacata ttcatccgcc caagaagggc tacaatgtgg atcatgaagg caagaaaaac
     2401 gggctgcatc cggtcaaagt cattccgggc actcgattag cgaaaatcct cggcaagggt
     2461 cgcaaggtgg tggccacaca acatcaccag ggcgtccgga cactggcccc cgatttcatt
     2521 cctgccgcta tcgccagcga cggactcatt gaggcgatgg aacatcccaa ataccctcat
     2581 atatttaccg ttcagtggca tccggaacga caactccgca gccaaaccac acaacggctc
     2641 ttgcgggcct tcgtccgatc ctgccaacac taccagcgaa caagacaccc ataatcgggc
     2701 ctcaactcgt cctatggtcc aacccaaaaa atcgtaaaca atacgggcaa ggaatcagca
     2761 atgagacggg aaagagtagc aggctgtgtc atgcggcaat ctgaaaggca ctcatccggc
     2821 atgagacatt ggcacggtgt accggcaagc tcccacctca ttatcattct gagccggaga
     2881 agaagaatct g
//
LOCUS       DATIAL010000118         3941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_343891, whole genome shotgun sequence.
ACCESSION   DATIAL010000118 DATIAL010000000
VERSION     DATIAL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343891"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VLA60_03790"
     CDS             <1..711
                     /locus_tag="VLA60_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HSF08510.1"
                     /translation="FFSNYGSAYTIRIGDITPARSGFGDPAQKLFKFKDGERIIDGWS
                     FDPRVLSTTPSSPAPAKDRSLPLFDTVPGGEYGEAVAITSSGMGVRFDLATFQEPSTR
                     LGRKFCKLKEGEEVVGVSHVTAPLKKTVVAVVSGRGRALLCQAEEVAQLAGPGRGVIV
                     MKLDQNDTVVGFKLLSSKEEELVVLKEDGGTLPISLRKYQVVGRGGKGHPLMKRGRLT
                     GMVQGELTIPIFQTEATL"
     gene            780..2723
                     /locus_tag="VLA60_03795"
     CDS             780..2723
                     /locus_tag="VLA60_03795"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HSF08511.1"
                     /translation="MGKTYAASDIVVLEGIEPVRRRPAMYIGGTDNTGLHHLVWEILD
                     NAIDEVMNGYATHIIVELDKSREGLRITDNGRGIPVDKHPKHKKSALEIIMTTLHAGG
                     KFDTGSYIHSGGLHGVGASVVNALSRHLEVQVKRDGKEWEQTYQAGVPQGPVKVLGNA
                     RGTGTSVYFRPDPKIFGKTIQFDPAVLQETLDAKAYLHKGLKLTFKDGTTGETHQFQH
                     EQGIQEYLRKLVADRGRKTTVDSVFYLERQLKNGGSGSEGAGQGVKMELALQWTDEPA
                     EFVKSYVNGVATPNGGTHEMGLRGGIVKAVRNYIDTHNLTPKGLSLQAEDIREGMAAV
                     LSVLVLHPQFQGQTKERLNNPEVQAQVDSLVRPALETYLNENQSIAETLVGRMILAAR
                     AREASREASKAVMRKSAISHRLNLPGKLADCQSTDPTISELFIVEGDSAGGTAKQGRD
                     LKNQAILPIRGKVLNTEELTLAKVVENKELADVVKVLGCGIGKDFDLKKLRYHKIILL
                     MDADIDGYHISTLLLTFFFRHMPELIRQGHIYIAQPPLYRIDIGKDIHWAGTDEEKDR
                     IVAQARSNAKPTISRFKGLGEMFPQQLKVTTLHPATRRLLKVDLLDELHTDRTFQDLM
                     GKRTEARYEFLMANAGLADNLDV"
     gene            2838..>3941
                     /locus_tag="VLA60_03800"
     CDS             2838..>3941
                     /locus_tag="VLA60_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSF08512.1"
                     /translation="MARQQTEIALDQVKQYHEQTKHEFNRYARSLGYLDWANQPDPFR
                     RFDGAPLISLPHLTLEESPHSPPYEALFGSPSIPSQPITINTLSRFFEYALSLTAWKE
                     YNGTRWALRSNPSSGNLHPTEGYIFVSGASHLALEPGLYHYAPKEHALEHRWVLPFDV
                     AQAVLQEMPSEGFLVGLTSIHWREAWKYGERAFRYCQHDTGHAIGTLRIAAATLGWNL
                     LVLNGTTDETIARLLGLTRLQDFHQAEREYPELLAAVWPGNLHPSPTLNLAFHDPDDS
                     LLSSGEWKGRANRLSKDEPVLWEIIDTVTEASWKSTQENARMVFHTPTAHTSNHEGVR
                     PGLLAGHIIHQRRSAVAFDGRTSLSAGRFFTMLQ"
BASE COUNT         1014 a         1058 c         1029 g          840 t
ORIGIN      
        1 ttcttttcaa actacgggag cgcgtacacc attcgcatcg gcgatatcac gcccgcacga
       61 agcggatttg gagatccggc acaaaagctg ttcaaattta aagatggtga acgcatcatt
      121 gacggctgga gcttcgaccc tcgggtgctc tcaacgaccc cgtcttctcc tgcacctgca
      181 aaagatcgct ctttgccgct attcgacacc gtgccgggcg gggagtacgg agaagccgta
      241 gccattacct caagcggaat gggggtccgg tttgacctcg ccaccttcca ggaaccttcc
      301 actcgtctgg gccggaagtt ttgcaaactg aaggaagggg aagaagtcgt cggggtcagt
      361 catgtgacgg ctcccctcaa aaaaaccgtg gtggccgtcg tctcgggtcg aggacgcgct
      421 ttgctctgtc aggcggagga ggtcgctcaa ttggccggac cgggccgtgg agtcatcgtc
      481 atgaaattag accagaacga taccgtcgtt ggcttcaaat tattatcatc caaggaagag
      541 gaactggtgg ttctgaaaga agacggcggc acccttccca tctcccttcg caaatatcag
      601 gtagtgggac gcggagggaa agggcatccc ttgatgaagc ggggtcgcct gacaggcatg
      661 gttcaggggg aattaaccat tccgatcttt caaaccgaag ccaccctgta actccctcat
      721 agaccccata cccaatagtg tgaaattaat gcttcccatt cactcacgag gatctgttca
      781 tgggtaaaac atatgctgcc agtgacattg tggtgttgga gggtattgag cccgtccgac
      841 gccgccctgc gatgtatatc ggaggcaccg ataacacagg cttgcaccat ttagtctggg
      901 aaattctgga taacgccatc gacgaagtca tgaatggcta cgcaacccac attattgtgg
      961 aactggataa atcacgagag ggtcttcgca tcacagacaa cggacgtggc attccagtgg
     1021 acaaacatcc caaacacaaa aaatcggcgt tagaaatcat catgacgacc ttacatgcag
     1081 gaggaaagtt tgatacggga agttatattc attccggagg cttgcatgga gtcggtgcct
     1141 cggtcgtgaa cgcgttgtcc cgtcacctgg aagtgcaggt gaaacgtgac ggcaaggaat
     1201 gggaacaaac gtatcaagcc ggtgtgcccc aaggtcccgt caaagtcctg ggcaacgcac
     1261 gaggaaccgg cacctcggtc tattttcgtc cggatcccaa aatcttcggc aaaaccattc
     1321 aattcgatcc cgccgtgctg caggagacac tggatgccaa agcatacctt cacaaaggcc
     1381 tgaaacttac cttcaaggat ggcaccaccg gcgaaactca tcagtttcag cacgagcagg
     1441 gcatccagga atatctgcgc aaattagtcg cagatcgcgg gcgaaagacc accgtcgatt
     1501 ctgtgtttta cctggaacgc caattgaaaa atggcgggtc aggctctgaa ggagccgggc
     1561 aaggcgtaaa aatggaactc gccctgcaat ggaccgatga accggccgaa ttcgtcaaaa
     1621 gttatgtgaa tggggtggca actccaaatg ggggcactca tgaaatggga ttacgtggcg
     1681 gcattgtgaa agccgtacgc aattacatcg atacccacaa tctcaccccg aaaggtcttt
     1741 ccctgcaagc ggaagatatc cgtgaaggca tggcggccgt gctgagcgtg ctcgttttac
     1801 accctcagtt tcagggccaa acaaaggaac ggctcaataa tccggaagtg caggcgcaag
     1861 tggatagtct cgtccgacct gccttggaaa cctatttgaa cgaaaatcag agtattgcgg
     1921 aaactctcgt ggggcgcatg attctggccg cgcgagcccg tgaggcctct cgagaagcgt
     1981 ccaaagcggt gatgcggaag tctgccatca gccaccgcct caatctcccg gggaaactgg
     2041 ctgactgcca aagcacggat ccgactatca gcgaactgtt tatcgtcgag ggcgactccg
     2101 caggcgggac agcgaaacaa ggacgcgact taaaaaatca ggccattctt cccatacggg
     2161 gaaaagtgct gaatacggaa gaactcacac tggctaaagt ggtagagaac aaagagctgg
     2221 ccgatgtcgt gaaggtgtta ggctgtggaa tcgggaaaga tttcgatctc aaaaagctcc
     2281 ggtatcataa aatcattttg ttgatggatg ccgatattga cggctatcac atcagcacat
     2341 tactgctgac atttttcttc cggcacatgc cggagctcat ccgccaaggc catatctata
     2401 ttgcccaacc tccgctctac cggatagata tcgggaaaga catccactgg gccggaaccg
     2461 acgaggagaa ggatcgaatc gtggcccagg cccgcagtaa tgccaagccg acaattagcc
     2521 ggttcaaagg attaggggag atgtttcctc aacagctgaa ggtcacgacc ttacatcccg
     2581 ccactcgtcg actactaaag gtcgatctcc tggatgaact acacaccgac cgaacctttc
     2641 aggacttaat gggtaaacgc acagaagccc ggtatgagtt tctcatggct aacgccgggc
     2701 tggctgataa cctcgatgtg taaaaaacac gagtatatga atagggcgaa aaaaggagaa
     2761 cctaattttg gtggtgagac gacgagcttt ttcacctcgc gtctaacgat ggtacactac
     2821 cttctatgca taacgacatg gcccgacaac aaaccgaaat agccctagac caggtcaaac
     2881 agtaccacga gcaaaccaaa catgagttca accggtacgc ccgctcgtta ggatatttgg
     2941 actgggcgaa tcagccggat ccgtttcgtc gatttgacgg cgcacccctc atttcacttc
     3001 ctcatctcac actcgaggaa agtccgcatt ctccaccgta tgaggcgcta tttggttcgc
     3061 cttcgattcc ctctcaacct ataaccatca acaccctctc ccgctttttc gaatatgcgt
     3121 tatcgctcac cgcatggaaa gaatacaacg ggacccgctg ggcgctgaga agcaatcctt
     3181 ccagcggcaa cctccaccct accgaaggct atatctttgt gagtggtgcg tcccaccttg
     3241 cactggaacc gggactttac cattatgctc ccaaggaaca tgcgctcgaa caccggtggg
     3301 tacttccttt tgatgtcgct caagcggtcc tccaggaaat gccttctgag ggatttttgg
     3361 ttggcctcac gtccattcat tggcgggaag catggaaata tggggaacgg gcctttcgct
     3421 actgtcaaca tgataccggt catgccattg gcacactacg cattgctgca gcgacgttag
     3481 gctggaatct gttggtgttg aacggcacaa cggacgaaac tattgcacga ctcctgggtc
     3541 tgacgcgact gcaggacttc catcaggccg aacgtgaata tccggaactt ctggcggcag
     3601 tctggcctgg aaatctccat ccctctccca cgctgaatct ggcatttcac gatcccgatg
     3661 attccctgtt gtcatccgga gagtggaaag gcagggcgaa ccgattgagc aaagatgaac
     3721 cggttttgtg ggaaattatc gatacggtca cagaagcgtc ctggaaatcg acccaagaaa
     3781 acgcacgtat ggtctttcac accccgactg ctcacacgag taaccatgag ggggtaagac
     3841 ccgggctcct ggccggccac attatccacc aacgacgaag tgcagtcgcc ttcgacggac
     3901 gaaccagcct ctccgccggt cgttttttca cgatgctgca a
//
LOCUS       DATIAL010000119         8371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_774041, whole genome shotgun sequence.
ACCESSION   DATIAL010000119 DATIAL010000000
VERSION     DATIAL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8371
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774041"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(411..1670)
                     /locus_tag="VLA60_03805"
     CDS             complement(411..1670)
                     /locus_tag="VLA60_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HSF08513.1"
                     /translation="MKASSPHHLLIGMLLGIVVGILLGGLAPSIGLSLAFIGDLFLQA
                     LFALVVPLVMSSMIVGIASLGDVRQLGSIGLRTILFFMTTTGLAVLVGLILVIVIHPG
                     TSTEQASPDTPTVQTTNLSERMQDKPSTLGDLFQSILTSLVPKNLFAAMAETQILPLI
                     VFALVFGAVLTTIGEKGTLVIRVFEGINEAMMAIVHLLMWIAPVGIGALLAGRLGEAG
                     GFTGFLPQLMSLGTYVGTVLLALAIHGLVTLPLILRFLGQRPVPAYAKAMSTPLMTAF
                     STSSSSATLPLTMEGLIEEAKVSRRVVSFVVPLGATINMNGTALYEAVAAMFIAQTYG
                     IEMGFGETIVVLLTATLSAIGAAGIPEAGLVTMVIVLKAVDLPIEGISLILVVDWFLD
                     RCRTTVNVWGDAVGAAVIDRWESANKT"
     gene            complement(1744..4596)
                     /gene="gcvP"
                     /locus_tag="VLA60_03810"
     CDS             complement(1744..4596)
                     /gene="gcvP"
                     /locus_tag="VLA60_03810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HSF08514.1"
                     /translation="MTTFAHRHIGSSESQIQEMLGALGLSSLDALMQATVPKDIRLAR
                     SLDLHPAQSEEEVLAELRHLSARNRVYRSFLGMGYYDCLTPPVIGRNILENPAWYTQY
                     TPYQAEIAQGRLEALLNFQTMVADLTALPLANASLLDEATAAAEAMSMCRALSTLQGR
                     TFFVAEDCHPQIIGVLQTRAKPLNISITIGNPDTLNFSNDETFGILLSYPSTDGTLRS
                     YEALVQKAHESGALVVVSTDLLALTLLVPPGEWGADIAVGSSQRFGVPLGYGGPHAAF
                     LATKEAFKRHIPGRMVGVSKDAHGQTAYRLALQTREQHIRRDRATSNICTAQVLLANM
                     AGMYAVYHGPEGLKNIATKVHHLTALTAEGLRRLGCQLDSNAFFDTLRVRCPHLSPAQ
                     IIKNGQQSFINLRQFPDGSFGIALDEATTITDVSQLLTLFTDNTSLPFSLDDLAEQLG
                     SSIPNTFQRHSPFLTHPVFHDYHSEHELLRYMYRLQSKDLSLVHSMIPLGSCTMKLNA
                     TAEMVPVTWPEFSRIHPFAPSEQAQGYQLLFRQLEAWLAEITGFEAVSLQPNAGSQGE
                     YTGLMVIRAYQEEQGQKGRNICLIPVSAHGTNPASAVIAGLKVVPVACDNHGNIDIQD
                     LEAKSAQYQDTLSCLMVTYPSTHGVFEEGIRKVCEIVHTHGGQVYLDGANMNAMVGLV
                     HPGAFGADVCHLNLHKTFCIPHGGGGPGMGPIAVASHLAPFLPGHPLVQVGGPKGIGP
                     IAAAPYGSPNILPISWVFIKLMGGEGLTKASQVAILNANYMANKLDKYYSMLFAGKNG
                     MAAHEFILDLRPFKKSADIEVEDVAKRLMDFGFHAPTISWPVPGTMMIEPTESESKAE
                     LDRYCDALILIRQEIAEIEAGRLPRHNNPLKHAPHTIETIAQSDWPHPYSRETAAFPA
                     PWLRQHKFWPSVSRIDNVYGDRNLICTCPPMDTYSS"
     gene            5047..6354
                     /locus_tag="VLA60_03815"
     CDS             5047..6354
                     /locus_tag="VLA60_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSF08515.1"
                     /translation="MIVLSNIHKLYDGTSATTRSIHERVDVWIDEGTIKAVSPHNPAR
                     PQGSDVHRIDCTTYTVTPGLIDCHGHVTLWGVGSAELDRMNSPEAPFWAERILYRTLV
                     QGGVTTLRDVGGATSVLKRLVDQGVVLGPRLKLAICMLSTTGGHADFRGPDRCCGELS
                     RLWPAGPGRPSSIVDGPWECRKRVREIAACGGDLVKICASPGVVSPTDHLEHRDFTLE
                     EMEAICDEAAGRGMYVAAHAHSRNGIKLAIQAGVKDIQHISFMNEDLAELAYAKGCVV
                     TPTAWVGQKLAQSEGFNAFIRAKVQQVAESHAHAVKSAQASGLKLLAGTDPVLPDMHG
                     RNYMELVALIAEGVPPLAAWFGATGLAANQLGQTDAGTLVEGKRADILVCREDVVEDP
                     SRFEYGALVEVLKDGWAYRNGLPGVRQRTFTDCVDLALGSSSL"
     gene            complement(6401..7003)
                     /locus_tag="VLA60_03820"
     CDS             complement(6401..7003)
                     /locus_tag="VLA60_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08516.1"
                     /translation="MAKELPVIPFPKQSESSEQEELIYSRVFCTREDSPPLKLLLDFL
                     KSKNQIPLIPKMDPAALEDWDWVHVSLGYSRERKPIRLFCLRDRGTYQDVFEGEKTSF
                     FNRISVFDDIEAGIARECISKARFIATTQMVKKDMTDEGYDFNGWILEFFQENCNGIV
                     QIDGQGFYSPKGELIVDMEEVAETGDDIAPTPKADTQTLA"
     gene            7339..7629
                     /locus_tag="VLA60_03825"
     CDS             7339..7629
                     /locus_tag="VLA60_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HSF08517.1"
                     /translation="MGKAMTKSQIADHLAKKADVTKKIATQILDDFAALAYKEAKNAF
                     VVPGVGKLVLAHRKARMGRNPQTGAAIKIPAKRVVKFRVAKAAKDAILGVKK"
     gene            8022..>8371
                     /locus_tag="VLA60_03830"
     CDS             8022..>8371
                     /locus_tag="VLA60_03830"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF08518.1"
                     /translation="MASSASRLGEIFLHRGDLSKARECYEQATAEARQLGSLKALSDA
                     LGNLGNVSALLGHYEQAEACYREVLEIQRTLQEGHAIGETLVNLGSLKADRGEPETAH
                     AFYLEAIDYLAPLG"
BASE COUNT         2050 a         1941 c         2340 g         2040 t
ORIGIN      
        1 gggttccaga tggaaagtcg ggaagcgaat gatgacccgc gcgctttcgg atcatgcaga
       61 atatagggtt cctcgctcat tcatgtgtgt cctgaaaagg tttgtttccc ctggtccgac
      121 gcctgccgcc cggccctttg gatgaacgct ctcatgtgcc agcgagcctt gtttaagtgc
      181 agcgagttgg ctcgcccttc cgtgccttgc gtccatctca ttgaatgaag ccggacgggg
      241 cgtcaatggg ttttggctcc ttttgccgaa acaaaagggg ctcggctgcc gggccgaaaa
      301 ccggcttgcg tcaatttaaa atgggatcaa catttactag gatccctaga gtgggcattc
      361 ctttggggta gttacgggaa cgaaaaggct ggtttagacg aagagaagct ttatgtcttg
      421 ttcgcagatt cccagcgatc aatgactgcg gccccgacgg catcacccca gacattcacc
      481 gttgttcggc agcggtccag aaaccaatcc accactaaga tcagggaaat gccttcaatt
      541 ggcaggtcca cggctttgag cacaatgacc atcgtcacga gtccggcctc tggaatacct
      601 gcagccccta tcgcagagag agtggcggtg agcaagacca caatggtttc cccaaaaccc
      661 atttcaatcc cgtaggtttg tgcgataaac atggcggcca cggcttcata aagcgcggtc
      721 ccattcatgt tgatggtagc tcctaacggg actacgaagc tgaccacgcg tcgagaaacc
      781 ttcgcttcct caatcagtcc ttccatagtt agtgggaggg tggccgaact ggaactggtt
      841 gagaaggcgg tcatcagtgg ggtactcatg gctttggcat aagctgggac gggacgttga
      901 ccgagaaatc gtaagattaa aggaagcgtg accaagccat gaatggctaa ggcaaggagc
      961 actgtaccca cataggttcc taagctcatc aactgaggca aaaatcctgt gaacccccct
     1021 gcttcgccta atcgtccagc taacaaagcg ccaatgccta ccggggcgat ccacatgagc
     1081 aggtggacga tggccatcat agcctcgttg atcccttcaa agacccgaat cactaaagtg
     1141 cctttttctc caatggtggt gaggacggca ccaaagacta aggcaaagac gatcaacggc
     1201 aagatttgtg tttcggccat ggccgcgaat aaatttttgg ggactaagct cgtgaggatg
     1261 gattgaaaca aatcacccag ggtggagggt ttatcttgca tacgttctga caggtttgtg
     1321 gtctgtaccg ttggtgtgtc agggctggct tgttcggttg atgttccagg gtgaatgacg
     1381 atgaccaaga tcaagccgac caacaccgcc agtccagtcg ttgtcatgaa aaataggatg
     1441 gtgcgaaggc caattgatcc gagttgccgc acatcgccta agctggcaat gccgacaatc
     1501 attgagctca tgaccaaggg gacgacgagc gcgaacaagg cttgtaaaaa caggtcaccg
     1561 ataaatgcga ggctcaatcc gatggatggt gcaagcccac ccaggagaat gccgacgaca
     1621 atgcccaaaa gcatgccaat gagcagatga tgcgggctgg aggctttcat gtggttggat
     1681 gctgtttccc catcaagaat aggaacgggc tatccaatgg tcgatgaaaa gcctagagta
     1741 agtctaggat gaataggtgt ccatgggtgg acaggtacat atcagattcc ggtctccgta
     1801 aacattatct atcctcgaaa cagatggcca aaatttatgc tgccgtaacc agggggctgg
     1861 aaatgccgcg gtttcacggg aataggggtg cggccagtcg gattgagcga tggtttcaat
     1921 agtatgaggc gcatgcttta aaggattatt gtggcgaggg aggcgacctg cttcaatctc
     1981 ggcaatttcc tggcggatca ggatgagcgc atcgcaatac cggtctaact ccgccttgga
     2041 ttcactttcg gttggctcga tcatcatggt gccggggacc ggccatgaaa tcgtgggagc
     2101 atggaatccg aaatccatga gccgtttggc cacatcttcc acttcaatgt ccgctgattt
     2161 tttgaaaggg cgaagatcta aaataaattc gtgggccgcc ataccgtttt taccggcaaa
     2221 gagcattgaa taatatttat ccaatttgtt ggccatatag tttgcattga ggatggccac
     2281 ttgggaggct tttgtgagtc cctctccgcc catgagttta atgaagaccc aagagatagg
     2341 aagaatattc gggctgccat atggggcagc ggcgatgggc ccgatacctt ttggtcctcc
     2401 tacctgtacc aaaggatgac ctggtagaaa gggggccaag tgagaggcaa cggcaatggg
     2461 gcccatccct gggcctccgc caccgtgggg gatacaaaag gttttgtgca ggttgagatg
     2521 gcacacatcg gccccgaatg cacctggatg caccaagcca accatggcat tcatattggc
     2581 gccatctaaa tagacttggc ctccatgtgt gtgcacgatt tcacagacct tgcgaatgcc
     2641 ttcttcgaat acgccatggg tagatgggta tgtcaccatt aaacaggaga gggtgtcctg
     2701 gtattgggct gacttggctt ctaaatcctg aatgtcgatg tttccatggt tatcacaggc
     2761 gacaggaacg accttcaatc cggcgatgac cgcactcgcc gggtttgtcc cgtgcgcgga
     2821 caccggaata agacagatat tccgtccttt ttgcccctgc tcctcttggt aggcccgaat
     2881 aaccatcaat ccggtatatt ctccttgaga gccggcgttg ggttgaagtg aaacagcctc
     2941 aaatcccgtg atttcggcga gccatgcctc caattgccga aacaacaact gatatccttg
     3001 cgcctgctca ctgggggcaa aggggtgaat ccgactgaat tcgggccatg tcacgggaac
     3061 catctccgct gtcgcattga gcttcatggt gcatgatccc aagggaatca tggaatggac
     3121 gagggagagg tccttcgatt ggagccgata catatacctg agcaattcgt gctcggaatg
     3181 atagtcgtga aaaacaggat gtgtgaggaa aggagaatgt cgctgaaagg tgtttgggat
     3241 ggaggaaccg agttgttccg ccagatcgtc taaggagaag ggaagcgatg tattgtcggt
     3301 gaaaagggtc aggagctgac tcacgtctgt gatggtggtc gcttcgtcca acgcgatgcc
     3361 aaaggacccg tctggaaatt ggcgaagatt gatgaaactc tgttgaccat tcttaatgat
     3421 ttgggccggg ctcaggtgcg ggcaccgaac ccggagggta tcaaaaaagg cattggagtc
     3481 gagttgacat cccaagcgac gaagtccttc ggctgtgagc gccgtaaggt gatgaacttt
     3541 ggtggcgatg ttttttaatc cttccgggcc atggtagacc gcatacatgc cggccatatt
     3601 tgccagcagc acctgtgcgg tacaaatatt actcgtggct cgatcgcgcc ggatatgttg
     3661 ctctcgggtt tggagggcca ggcggtatgc cgtttggccg tgagcgtctt tggatactcc
     3721 caccatcctg ccggggatgt gtcgtttaaa ggcctctttt gtggccagaa atgccgcgtg
     3781 gggaccgcca tagcccagcg gaaccccaaa tcgttgcgat gaccccaccg caatatccgc
     3841 tccccattct cccggaggca ctaaaagggt taaggcgaga agatccgtac tgaccacgac
     3901 gagtgccccg gattcatgcg ccttctggac cagggcttca taggagcgga gcgtcccgtc
     3961 agtggacgga taggagagga gaatgccgaa ggtctcatcg tttgaaaaat ttaaagtatc
     4021 tggattccct atggtaattg agatattcaa gggcttggcc cgagtctgca ggacgccaat
     4081 gatttgcggg tgacaatcct ccgcgacgaa aaaggtacgt ccctgcaggg tgctgagtgc
     4141 ccgacacata ctcatggctt ccgccgccgc cgtggcttcg tctaacagtg aagcattagc
     4201 cagaggaagc gcagtcagat cggcaaccat ggtttggaaa ttgagtaacg cctccaatcg
     4261 tccctgggca atttccgctt gatagggggt atattgggta taccaggcgg gattttccag
     4321 aatgttccgt ccaatcaccg gtggggtcag acaatcgtaa taacccatgc cgagaaatga
     4381 tcgatagacc cgattccgtg cgctgaggtg gcggagttca gcaagcactt cttcttccga
     4441 ttgtgccggg tgtaaatcca aggaccgggc gaggcgaata tctttcggaa ccgtggcttg
     4501 catgagggca tcgagggaag agagcccaag cgctcccaac atttcttgaa tttggctctc
     4561 actggatccg atatggcggt gggcgaacgt ggtcatgcct ggctacacct catgtgatgt
     4621 gaaggttatc cctcaagcag atgggaaatt tgttgcgtta accgtattga ggaaatgatg
     4681 atttctgttc gggaccaatg gtgaaaaact tcgagaggta ttcggaaaga aaacataact
     4741 gacaaatgcg tctctcatga atagagaaaa gaaatgagcc aatatcttcg aagattatca
     4801 tgtttattat gaatccgcaa aatttccatg cccacaaatg ttattgatgt gatttagtac
     4861 caggtgtgaa agaatgaacg cgaccagatt aaggggtgct cagaggggag acactgaata
     4921 tttcctgttt gtcaggagga gttgcccggg aatgagcgtg atcgaatgtg ttgcagatat
     4981 tgacggatat acggtaaaga aaaaatgaag ggttcataag atttatatct tgaagagagg
     5041 aatgtaatga ttgttctctc aaatatccac aaactctatg atgggacgtc cgcaacaacc
     5101 aggtctattc acgaacgagt tgatgtgtgg atagatgagg gaaccatcaa ggccgtttct
     5161 cctcataacc ccgctcgtcc tcagggatct gatgttcatc ggatcgactg cacaacctat
     5221 accgtcaccc caggacttat cgactgtcat gggcatgtga ccctttgggg agtgggaagc
     5281 gctgagctgg accgaatgaa ttcccctgag gctccgtttt gggctgaacg tatcttatat
     5341 cgcaccttag ttcagggagg cgtgacgacc ctgcgggatg tgggtggagc tacttctgtt
     5401 ttgaagcgac tcgtggatca gggggtggtc ttgggtccac gtctcaagtt ggccatttgc
     5461 atgctttcca ctactggagg gcatgcggat tttcgagggc cggatcggtg ctgtggggag
     5521 ctttcacgtt tgtggcctgc tggtcccggt cgtccgtcca gtatcgtcga tgggccgtgg
     5581 gaatgtcgaa agcgggtaag agagattgcc gcctgcggtg gcgatcttgt gaaaatttgt
     5641 gccagcccgg gtgtggtgtc tcctaccgac catttggagc atcgggattt tacccttgag
     5701 gaaatggaag cgatctgtga cgaagccgct gggcggggga tgtatgtggc ggcccatgcc
     5761 catagtcgga atggtatcaa gttggccatt caggctggag ttaaagatat ccaacacatt
     5821 tcatttatga atgaggacct ggccgaattg gcctatgcga aggggtgtgt ggtgactccg
     5881 actgcatggg tggggcaaaa attggcccag tcagaggggt tcaatgcctt cattcgggcc
     5941 aaagttcaac aggtggccga gagccatgct catgcagtca aatctgcgca agccagcggg
     6001 ctcaaactgt tggctggcac tgatccggta ttacccgata tgcatggccg gaattacatg
     6061 gagttggtgg cattgatcgc agagggcgta ccgcctctgg cagcatggtt cggagcgacg
     6121 gggttggcgg caaatcaact tggccaaacc gatgcaggaa cactcgtgga ggggaaacgc
     6181 gccgatatcc tggtgtgcag ggaagatgtg gtggaggacc cgtcgcgatt tgaatacgga
     6241 gccctcgtgg aggtgttgaa agatggctgg gcctatcgca atggacttcc gggtgtgagg
     6301 caacggacct ttacggattg cgtggatttg gccctcggat caagctcttt gtgaggctct
     6361 tcttcagttc cttcggagca ggattgaact ataaagtttc ttatgccaat gtttgggtat
     6421 cagctttcgg tgtaggagct atatcatcgc cagtttccgc gacttcttcc atatccacaa
     6481 tgagttcgcc cttcggagaa taaaatccct gtccatcgat ttgcacaatg ccattgcagt
     6541 tttcttgaaa aaattccaga atccatccgt taaaatcata gccctcatca gtcatatctt
     6601 ttttcaccat ttgggtggtg gcaatgaagc gggctttgga aatacattcc cgggcaatcc
     6661 cggcttcaat atcatcaaag acggatatgc ggttgaaaaa tgaggttttt tctccctcaa
     6721 acacgtcctg ataggtaccc cggtcgcgca ggcaaaataa ccggatgggc ttcctctccc
     6781 ggctataacc aagggataca tgaacccaat cccaatcttc cagtgcggcc ggatccattt
     6841 tgggaatcaa tgggatctga tttttggatt tgagaaagtc cagaagcaac ttcaggggtg
     6901 ggctatcctc ccgcgtgcaa aacaccctgg aatatatcag ctcttcttgt tctgaggatt
     6961 ctgattgttt ggggaatggg ataacaggta attcttttgc catgatgtat gcctcgtgtg
     7021 tgttgtgcca attgcacgaa tgactaaagt aagtaaaatg tgtattttgg ctactttacc
     7081 gtgacaaaag ccgaggcagc aagacagaaa aatcattggg aaacttatga caaaagaata
     7141 ttcgccgcac tgggcttgtt ttttgttgac agtttttgag gcattcggta gactctgccg
     7201 ccgttgtgac agaggggaaa ggtgttcccg cgtttaaccc ttcttccaaa atgacagaca
     7261 aggcaatcct tccttggaat tctagaatca acaaatggtg tctcttacta aaacaattca
     7321 aatgaggagg aaatgtgtat gggaaaggcc atgacaaagt cgcagattgc cgatcacctg
     7381 gctaaaaaag cggatgttac caagaaaatt gccacgcaaa ttcttgatga ttttgccgcg
     7441 ttggcttaca aggaagccaa aaatgcgttt gtggtacctg gagttggcaa gttggtcctt
     7501 gcgcaccgaa aagccagaat gggcagaaat ccccaaactg gagctgcgat taaaatccca
     7561 gccaaacgtg tcgtgaaatt ccgcgtagcc aaggcagcta aagatgcgat cttgggggtt
     7621 aaaaagtaac tgtacccttc tcaaggcatt gtcctgtcgg ctttcccctc tctaagccgg
     7681 ttggtttgaa tgaatgaaag ggcgagaccc tgccttttta ttttgggtgt ctctcaacag
     7741 ccgagtccag atgtctggac tcggctgttg tgtttttagg ttgggtgggc tctagacaga
     7801 ccaacgtaga tggaacgcgt ttcaggctga atgtcgcaac agcccttcga tccggtgagg
     7861 tctccctgat ccttgtcagg gaaaaggtgc aatcttgcta aggcgtgctt acatgtcaaa
     7921 ggttttcagg caggtacaag cctagagatt tggtcagaat gtatcttttc ccggctttcg
     7981 tctgagaact gaacaattgt tacgggattt tcattgctat catggccagc tctgcttctc
     8041 gtctcggcga aattttcctt catcgtggcg atctgtcaaa agcgcgtgag tgctatgaac
     8101 aggctaccgc agaggcaaga cagctgggat cactcaaggc cttgtcagat gctttgggca
     8161 atctgggaaa tgtcagcgcc ttgttaggac actatgaaca agccgaggcc tgttatcgag
     8221 aagtcctgga aattcaacga accttgcaag aagggcatgc catcggggaa accctggtga
     8281 atttaggaag tcttaaagcc gacaggggtg agccggaaac ggctcatgcg ttctatttgg
     8341 aagctataga ttatttggcc cctttaggcc a
//
LOCUS       DATIAL010000120         5697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_236627, whole genome shotgun sequence.
ACCESSION   DATIAL010000120 DATIAL010000000
VERSION     DATIAL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236627"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1626)
                     /locus_tag="VLA60_03835"
     CDS             complement(94..1626)
                     /locus_tag="VLA60_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSF08519.1"
                     /translation="MHNRIFGIETEYGLLIKADQPDVSSPWIAHRIIEHLFGRKKHGV
                     IDWHYRGHDEPPGNGGFLLNAGRMYVDMGHLEYASPECHSLWDLVAVDRAGDWLLQDC
                     LEELGLSEQVSIIKNNIDHETDATFGCHENYLVTRDFPFTYQGLGRLMPFLVTRQVFT
                     GAGRIGKARSVDGWIGLDDIEGRGGPRSTKSTEDGVIYQISQRPDYIVNDFFEWVQHN
                     RAIVNTRDEPLADPERFRRLHLLMGDSNMAEEATLLKMGTTGLVLQLIEEGHAPQGLD
                     FDDPVQTLRAISRDPQQKWMVTLSTGRHLSALDIQEQFWEAAAKQYAGQDEETDWVLA
                     HWESVLRDLREGYQAVVGRVDWASKLWLLENFRNAEKLEWDDPWLKSLDLEYHNVDPR
                     AGLYHGMTEEGDAPRFTTNDLVDLAKGQPPRNTRAFGRAEIIRHVVKEGWADFLEKAT
                     GHHDPRRPPYVINWSVLQLQGSPAFVMADPFRSYVRESREYCAEWSSRPSLESLSNKG
                     DT"
     gene            complement(1649..2467)
                     /locus_tag="VLA60_03840"
     CDS             complement(1649..2467)
                     /locus_tag="VLA60_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08520.1"
                     /translation="MYEEPYRWIEAVANRREYLDDQFTQGNPLVAVSFTQGILLLTFN
                     RGTDKIYEIYDRIALGGMGHPADLEKLRYTLLDMAHVEGFQRSPADVTASRLMKNGLA
                     PVIKQAFEEIFKAPYIAKILLAEVGASQERDRLFSINYDGMFEEQRGFGLLAASASIR
                     GLLTQEWKSQSLLSSLQSAVEQSLRLWGIASLLQTVPPSSDTEEEGTTSSADSSISTI
                     PEDEAIASRIRETLSKETLECVFLDRESGKMETYRALSQSELQSFIPPVFQPAI"
     gene            complement(2460..3254)
                     /locus_tag="VLA60_03845"
     CDS             complement(2460..3254)
                     /locus_tag="VLA60_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HSF08521.1"
                     /translation="MMGQQGDFLQVLRERGYAVEDLRQPFSGVTPSGHTEITAGTTVF
                     AIKYADGVLVAGDRRATAGNVVMYDRTDKVLELDRFSVLAIAGVPATAFEMARILEHS
                     FKYYRRSQLQDLSFEGKLRALSTLLKNNAPAALAGTGTVAPIFAGYDQLEGCGKIFFY
                     DILGAEFEAVEYAVSGSGSSTIRGILFYMNRWGNGPVAGMNEEEALTFVLRLMTSAAE
                     FDSATGGVDAQAQLYPIVKRISAEGVRAIPAERLKSLFSEKVLNHV"
     gene            complement(3257..3472)
                     /locus_tag="VLA60_03850"
     CDS             complement(3257..3472)
                     /locus_tag="VLA60_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HSF08522.1"
                     /translation="MRNEYGYAQAGGSLERKERPIDPLSKPSGPGDQESGPKRPETDS
                     PSRDNLMKRMRKVDPKQAEKYRQRTGE"
     gene            complement(3494..5017)
                     /locus_tag="VLA60_03855"
     CDS             complement(3494..5017)
                     /locus_tag="VLA60_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HSF08523.1"
                     /translation="MNPIRLFGIETEYGIAREDIETADPVVESMELVRAYLDGHFTRR
                     WDYRGEHPHEDQRGFRVTELAQDKEEDLFAEQDAHRPFSFHEMKSDLVLPNGARFYND
                     HTHPEYSTPECRSLKDMVAHDRAGERILWMAAQRRNQALGGPMVQLYKNNTDFHGHSY
                     GCHDNYLVSRELKFENLARGLMPFLVSRQLIAGAGKVGREAQERGFEPGGFQLSQRAD
                     FMEAELGVDTMHNRPILNTRDEPHANRERYRRLHLIVGDANMCEYATALKVGTTRVVL
                     DMIAGGLLPPCELDSPVQAIQMLSRDPELSTLVKRQSGGLISGVDIQQEYLNLARKFF
                     VDMDEETAWILGEWEQVLNLLERDRWQLVGKIDWVTKWWLLETFRQAERIGWDDPWLA
                     SLDLEYHNLDPDRGLFFGLEAEGRTTRISQEQEIHEAMKHGPRDTRGGLRGLCVQRFG
                     QDITSIQWEGVHFQGQNGGLHLDLLDHLEPVAVAQCRSVIEHAVTPFDMMESMVLKK"
     gene            complement(5076..>5697)
                     /locus_tag="VLA60_03860"
     CDS             complement(5076..>5697)
                     /locus_tag="VLA60_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF08524.1"
                     /translation="LDGIESLSQVVVILASNRPDMIDPAVLRPGRIDRKIKVGRPGRS
                     DVAEILRVYLTADLPYDTKKAAGSGQKDEAPVSQIIDGLLENLFQRSQENRVLAIRLR
                     NGRREILYRRDLLSGAVLAGIVRRAKERAIERAIQAGNQEASGLSLDDLLIASNEEFK
                     ESEIFPPDDSAEEWLKLLDYHPDQVVGVSSLQPGKAGEEHRTSQIV"
BASE COUNT         1384 a         1652 c         1314 g         1347 t
ORIGIN      
        1 taggaatttt aaaataccac tggtaaaaat ggcagtaaag gagcaaaaag atcaaaacgg
       61 ctcagtcccg gggtattttc cctcgaccga acattaggta tctcccttat tcgagagcga
      121 ttcaagggaa ggtcgagaag accattctgc gcaatattcc cgactttccc gcacataact
      181 gcggaaggga tcggccatga caaaagccgg actcccctga agttgcaaaa ccgaccaatt
      241 gatgacatag ggtggacgac gcggatcgtg atggcctgtc gccttctcta aaaaatccgc
      301 ccaaccttcc ttgacgacat gacgaataat ttctgctcgc ccaaacgcac gggtgtttcg
      361 tgggggttgc cctttcgcca aatcgaccag gtcattcgtc gtgaagcgcg gcgcatcgcc
      421 ttcttctgtc atgccatgat agagccctgc ccgcggatcg acattgtgat attccaaatc
      481 cagacttttc aaccagggat cgtcccattc caacttctcc gcgttccgaa agttctccaa
      541 caaccagagc ttggaggccc aatcaacccg gccgaccacc gcttgatagc cctctcgtaa
      601 atccctcagg accgattccc aatgggccag gacccagtcg gtttcctcat cctgaccggc
      661 atactgtttg gcggcggctt cccaaaactg ctcttgaata tccaaggctg aaaggtgccg
      721 gccggttgag agcgtaacca tccatttttg ctgagggtcg cgggagatgg ctcgcagagt
      781 ctggacagga tcatcgaaat ccaatccttg cggggcatgc ccttcttcga tcaattgcag
      841 aaccaagccg gtcgtcccca tttttaaaag agtcgcttct tctgccatat tcgaatcgcc
      901 catgagcaag tgcaaccgcc ggaatcgttc tggatcggcc aggggttcat cccgggtatt
      961 cacaatagcc cggttatgct gaacccattc aaaaaagtcg ttgacgatat aatccggacg
     1021 ctgggaaatt tgatagatca ccccgtcttc ggttgatttt gttgaccggg gccctccacg
     1081 tccctcaata tcgtccaagc caatccagcc atccacggaa cgcgccttcc cgattcggcc
     1141 ggcgcccgta aagacctggc gtgttaccag aaacggcatg agcctcccca gcccttgata
     1201 ggtaaacgga aagtcccgcg tgaccagata gttttcatgg caaccgaaag tcgcgtctgt
     1261 ctcatgatca atattgtttt taatgatcga gacctgttct gaaagcccaa gctcttccag
     1321 acagtcctgc aacagccaat ctccggcacg gtctactgcc actaaatccc acagcgaatg
     1381 acattcgggt gaggcatatt caagatgtcc catatcgaca tacatccggc ctgcattcag
     1441 cagaaagccc ccattgcctg gcggttcatc atgcccacga taatgccagt caatgacgcc
     1501 gtgttttttc cggccaaaca aatgttcaat gatccggtgg gcaatccaag gagaagacac
     1561 gtccggttga tcggccttga taagcaggcc atattcggtc tcaataccga agattcgatt
     1621 atgcatagga acctcgctct gccaggattt agatggcagg ttggaacacg ggaggaatga
     1681 aggactgtaa ttccgactga ctcagagcac gataggtttc cattttcccg ctctcccgat
     1741 ccagaaacac acattccagt gtttctttgg acagcgtttc acgaattcgt gaagcaattg
     1801 cctcatcctc tggaatggtg gagatggaac tgtctgctga agaggtggtt ccttcctctt
     1861 ctgtatctga agagggaggt acagtttgga gaagacttgc gatcccccac aaacggagac
     1921 tctgctccac agccgactgg agagaggaca gcagactctg gcttttccat tcctgcgtga
     1981 gcagcccacg gatggaggcg gaggctgcca gcaaaccgaa tccgcgttgt tcttcaaaca
     2041 tgccgtcata attgatgctg aacaggcggt cccgttcttg agaagccccg acttctgcca
     2101 atagaatttt ggcgatgtac ggtgccttga aaatttcttc aaacgcttgt ttgatgacag
     2161 gggccagacc atttttcatc agtcgggaag ccgtcacatc cgccggggac cgctggaatc
     2221 cttcaacgtg ggccatatcc aatagggtat accgtagttt ctccagatct gcaggatggc
     2281 ccatgcctcc gagcgcaatg cgatcgtaaa tttcatagat tttgtccgta ccccgattaa
     2341 aggtcagcaa caatatcccc tgtgtaaagg agacggcaac taacggattg ccttgtgtga
     2401 attgatcgtc caaatattcc cgccgattgg cgacggcttc aatccatcga tagggttctt
     2461 catacatgat tcaacacctt ttcagaaaac agtgatttca atcgttccgc gggaatggct
     2521 ctcacccctt ccgcggaaat gcgtttcacg atgggataga gttgcgcctg cgcatccact
     2581 ccacctgtgg cggaatcaaa ctctgcagca ctggtcatca gccgaagcac gaacgtcaag
     2641 gcttcctctt cattcattcc ggcaacggga ccattccccc accgattcat gtaaaacaaa
     2701 atcccccgaa tcgtggaaga cccggatcca gagaccgcat attccaccgc ctcgaattca
     2761 gctcctaaga tgtcatagaa aaatattttt ccgcaccctt ccagttggtc ataccccgca
     2821 aaaattgggg ccacggttcc ggttcctgcc aacgccgcag gggcattatt tttcaacaac
     2881 gttgataagg ctcgcaactt tccctcaaaa ctcaaatcct gtaattgact tctccggtaa
     2941 tacttaaagg aatgctctaa tattctggcc atctcaaatg cggtggcagg aacaccagca
     3001 atggctaaga cagaaaaccg atccaattcc aagaccttat cggtgcgatc atacatcacg
     3061 acatttccgg ccgttgcgcg ccggtcacca gccacgagga ccccgtcagc atacttgatg
     3121 gcaaagaccg tcgtcccggc tgtaatttca gtatggcctg atggcgtaac accactgaac
     3181 ggctgtcgaa gatcctcgac cgcatagcca cgctctcgta gaacctgaag gaagtccccc
     3241 tgttgaccca tcatccttat tccccggtcc gttggcgata tttttccgcc tgcttgggat
     3301 ccaccttgcg catgcgcttc atgagattat cacgggatgg tgaatccgtt tccgggcgtt
     3361 taggacctga ttcctgatcg ccaggtcccg atggcttaga aagtggatcg atcggccgtt
     3421 cttttcgttc aagacttcca ccggcttggg catatccata ttcgtttctc ataaatctca
     3481 tcctccacat gaactatttt tttaatacca tggattccat catatcaaag ggtgtcacgg
     3541 catgctcaat caccgaccga cattgagcga ccgccaccgg ctcaaggtga tccagcaggt
     3601 caagatgcag tcccccattt tggccctgaa aatgtactcc ctcccattgt atcgacgtaa
     3661 tatcctgacc aaatcgttgc acacacaacc ccctgagacc tccacgcgta tcacgcggcc
     3721 catgcttcat cgcttcgtga atctcttgtt cctgggatat ccgggtggtt cgcccctcgg
     3781 cttccaaccc gaaaaacagg ccacgatcag gatccagatt atgatattcc agatccaggc
     3841 tggcaagcca aggatcatcc cagccgattc gctcggcctg cctaaaggtc tcgagcaacc
     3901 accatttagt cacccaatcg atttttccca caagctgcca tcgatcccgt tcgagaaggt
     3961 ttaagacctg ttcccattcg ccaagaatcc aggccgtctc ttcatccata tcgacaaaaa
     4021 atttcctggc caaattcagg tactcctgtt gaatatccac cccggaaatc aggccaccag
     4081 actgccgttt cacaagggtg ctcaattcag ggtcacgaga gagcatctga atggcctgca
     4141 ccggggagtc aagctcacag ggcggcaaga gccctccggc aatcatatcc aacaccaccc
     4201 gcgtcgtgcc gaccttcaag gccgtggcat attcacacat attggcatca cccacgataa
     4261 gatggagtcg ccgatagcgc tcacgattcg catgaggttc gtctcgcgta ttgagaatcg
     4321 gtcggttgtg catggtatcc acgcccaact cggcctccat aaaatctgcg cgttgggaca
     4381 attgaaaacc gccaggctca aacccgcgtt cttgcgcttc ccgaccgacc ttgcctgcgc
     4441 cggctatcaa ttgtctgctg accagaaaag gcatgagtcc acgagccaaa ttctcaaact
     4501 tgagctcgcg cgagaccaga taattatcat ggcagccata actatgccca tgaaaatccg
     4561 tgttgttttt atacagctga accattgggc caccaagggc ttgatttcgg cgttgtgccg
     4621 ccatccaaag gattcgctcg ccggcccggt catgcgcgac catatccttg agagagcggc
     4681 attccggcgt ggaatactcc ggatgcgtat ggtcattata aaatcgtgcc ccattgggga
     4741 ggacaaggtc acttttcatt tcatggaagg aaaaaggtcg atgggcatcc tgctccgcga
     4801 acaggtcctc ttccttatct tgagctaact cggttacccg aaaaccccgt tgatcttcat
     4861 ggggatgctc gccccgataa tcccagcggc gggtaaaatg tccgtcaaga taggcccgca
     4921 ccaattccat ggattccacg acaggatcgg cggtctcgat atcttctcgc gcaatcccgt
     4981 actcagtctc aataccaaag aggcgaatag gattcatttg ttccaatctt tcagctgaga
     5041 aggtcgcatg tggcacagcg tgctggaaag ggagtttaca caatctgaga agtccgatgc
     5101 tcttctccag cctttccagg ctgcagggag gatactccga caacctggtc gggatgataa
     5161 tccaggagtt tcaaccattc ctcagctgaa tcatccggcg ggaaaatttc actctccttg
     5221 aactcctcat tgctggcaat gagtaaatca tcgaggctca gacccgaggc ctcctgattg
     5281 cccgcttgaa tcgcccgctc aatagcccgt tcttttgcac gccggacgat tcccgccaac
     5341 accgctccac tcaataaatc ccggcggtag agaatttcac gccgtccgtt acgcaaccga
     5401 atggccaaca ctcgattctc ctggcttcgc tgaaagagat tctctaacag tccgtcgatg
     5461 atctgcgaca ccggagcctc atccttttgt ccactaccgg cagccttctt cgtgtcgtaa
     5521 ggcagatcag ccgtcaaata aactcgcaat atttccgcca catcgcttcg tccaggtcgg
     5581 cccaccttaa tttttcgatc aattcttcca ggtcgcagca cggccggatc aatcatatcc
     5641 ggacgatttg aagccagaat aaccaccact tgggaaaggc tttcaatccc gtcgagt
//
LOCUS       DATIAL010000121         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_21368, whole genome shotgun sequence.
ACCESSION   DATIAL010000121 DATIAL010000000
VERSION     DATIAL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21368"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VLA60_03865"
     CDS             <1..240
                     /locus_tag="VLA60_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08525.1"
                     /translation="NEQRIIQLCQGATTLFCEAPFLEADRDRAANRYHLTARQAGILG
                     RKAGVEQLVVFHFSPRYTGQGDKLHQEAMEAFHEQ"
     gene            265..603
                     /locus_tag="VLA60_03870"
     CDS             265..603
                     /locus_tag="VLA60_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HSF08526.1"
                     /translation="MPEWVRYGLYFVLGGTLVSVSTYLGSHGRGFLAALASTLPIISG
                     VTFILIFINAGSTPTVSFAKHLIWLSPPWFVYVGAMIAFVPKLGFWPAYGLAIGLYLA
                     GVGLTRLFID"
     gene            657..1076
                     /locus_tag="VLA60_03875"
     CDS             657..1076
                     /locus_tag="VLA60_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08527.1"
                     /translation="MAEFIKPIIHAFLVCDTIIIDSLTGKKSIIGAFTHLWAKTFPCQ
                     HPQVGVYFSLTDAEGRYAFEIQLVYLDQDQIVGKGSLPPIDISNRLTTHDFGVNIPYL
                     VFPGPGRYEFRLYADGHFITQKDFQVIRQESSPSPQS"
     gene            complement(1147..1278)
                     /locus_tag="VLA60_03880"
     CDS             complement(1147..1278)
                     /locus_tag="VLA60_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08528.1"
                     /translation="MQRLRCEVEIAGGCGWFLSNAAGKGMFLPSRFADIDQVVRIVE"
     gene            1429..2364
                     /locus_tag="VLA60_03885"
     CDS             1429..2364
                     /locus_tag="VLA60_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HSF08529.1"
                     /translation="MAASHHQKGKLRLGCLFILILFVWGTGTVNGQVVGDGYNNSLIY
                     KIQSDTNTVYILGSIHVLAEEYYPLTRSFSYAYYNSQKVVFEIDPEILFSPDSFSKHE
                     KQYTLPKGKTLETVLSPRTYGLVKQKIQALGGNIQNFQQYKPWVLYLTLSGRFDSSID
                     FRPELGIENYFYRMAKDAGKPTGGLETIQDQLNVFDTLPLNIQDALLQESLAATGSKE
                     REQAFLHMVKDWHQGNLDGLEQLVESFKTYPLFYKNLLVQRNQNWIPQIESFLQEEKN
                     VLIIVGAAHLAGKDGLLALLREKGHHLERVSYAMP"
     gene            complement(2385..>2850)
                     /locus_tag="VLA60_03890"
     CDS             complement(2385..>2850)
                     /locus_tag="VLA60_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF08530.1"
                     /translation="FFGSVYCTKLALPFLKATQGRIVAISSVASLTGVPAHSAYCASK
                     HAMNGFFESLRIELTGTGVTVTIVAADFVQSEIHERSLGPHGQPFGRVLEGHARFLTA
                     RKCADMIIKGMTRRERLVFTSWRGQWGRWMKLMSPQMIDWMARKGMADADRP"
BASE COUNT          760 a          756 c          644 g          690 t
ORIGIN      
        1 aatgagcagc ggatcattca attatgccaa ggggccacaa cgctattttg cgaagcaccc
       61 tttttagaag ccgaccggga cagggctgcc aatcgctacc acctcacggc ccgccaggca
      121 ggaattcttg gacgaaaagc cggggtggaa caattggtcg tctttcattt ttctcccagg
      181 tataccgggc agggggacaa acttcaccag gaggccatgg aggcctttca tgaacagtaa
      241 tcacacatga catgaggagg gacgatgccg gaatgggtca gatatgggtt gtattttgtc
      301 ctgggcggaa cattggtgag tgtctccacg tatctgggaa gtcatgggag agggttcctt
      361 gccgccctgg ccagcactct gccgatcatc agtggagtga cctttatcct catcttcatc
      421 aatgccggat cgactccaac cgtctctttt gccaaacacc tgatttggct ttctccccca
      481 tggtttgtct atgtgggagc gatgattgca tttgtcccca agttagggtt ttggccagca
      541 tatgggttgg cgatcgggtt atatttggcc ggagtaggac tgactcgact gtttattgat
      601 tagtacagat atagagattg tgagaagcat ttggtcagat attgagaacc aagaatatgg
      661 cggaattcat taaaccgatt attcacgcat ttttagtgtg tgacaccatt atcattgata
      721 gtctgacagg gaaaaagagc atcatcgggg cctttaccca tctctgggca aagacatttc
      781 cctgccagca cccacaagtc ggcgtctatt tttcgctcac cgatgcggaa ggcaggtacg
      841 cattcgagat ccaactcgtg taccttgatc aggaccagat tgtcggcaaa ggttccctcc
      901 cgccaattga catttccaac cggttaacga cccatgactt cggcgtaaac atcccctatc
      961 tcgtgtttcc tgggccggga cgatatgagt ttcgtctgta tgctgatggt catttcatca
     1021 cccaaaagga cttccaggtt atccgacaag aatcttctcc ctcaccacag tcctgatttc
     1081 atattacttt ctcgtctatc gattccccct tcgaatggag cggaggacgg gaaaatccaa
     1141 caggactcat tccacaatcc ggaccacttg atcaatatca gcaaatcgac tgggcagaaa
     1201 catgcccttt ccggctgcat tcgacaagaa ccagccacac cctccagcaa tctcgacctc
     1261 acacctcagt ctctgcattt tacctttttc ctctagtcac cgatgaacta cccggtgaga
     1321 gacgaccaac ggccccaaga acccaatcaa gatgtcagac aacaggggaa aatcctttct
     1381 catcataaaa accggacaaa attacaactt cagagaggga gacttgcaat ggctgcatcg
     1441 caccatcaaa agggaaagct ccgcctaggc tgtctgttca tcctgatcct ctttgtttgg
     1501 ggcaccggaa cagtcaatgg tcaggtggtc ggagatgggt acaacaacag cctgatttat
     1561 aaaatccaat ctgatacgaa taccgtctat attttgggat cgatccatgt gctggccgaa
     1621 gaatactatc ctctcaccag atccttctcc tatgcctatt acaattcaca aaaggtcgtc
     1681 tttgaaattg atccggaaat attattttct cccgactcat tttctaaaca cgaaaaacag
     1741 tacacactcc caaagggcaa aacactggaa acggtgctct ccccaagaac ttatgggctg
     1801 gtcaagcaga aaattcaagc cttgggagga aacattcaga atttccaaca atacaaaccc
     1861 tgggtgctct atctcacgtt aagcggacga tttgattcgt ccatagattt ccggcctgaa
     1921 ttaggaattg aaaattattt ttatcgcatg gccaaggatg ccggaaaacc gaccggcggg
     1981 ctggaaacca ttcaagacca attgaatgtg ttcgacacac ttccgctcaa catccaagat
     2041 gctcttcttc aggaatcact cgccgccacc ggttcaaagg aacgagaaca agcgttttta
     2101 catatggtga aagactggca tcagggaaat ctcgacgggt tggaacaatt ggtggagtca
     2161 ttcaaaacct acccgctgtt ctacaaaaac ctgctcgtcc aacggaacca gaactggatt
     2221 ccccaaatcg aatcgttctt acaagaggag aaaaacgtct tgatcattgt gggtgcggct
     2281 cacctcgcgg ggaaagacgg attactcgcg ctgctgagag aaaaaggcca ccaccttgaa
     2341 cgagtgtctt atgccatgcc ctaaaaccgc aaaggagagt cccgttacgg ccgatcagca
     2401 tcggccatgc cctttcgggc catccaatca atcatctgcg ggctcatcaa tttcatccac
     2461 cgaccccact gtcctctcca ggacgtaaac accagtcgtt cccgcctggt catccccttt
     2521 ataatcatgt ccgcacattt cctcgcggtt aagaatctgg catgtccttc aaggactcgc
     2581 ccgaaaggtt gaccatgagg cccgagactt cgttcatgaa tttcggattg cacaaaatcg
     2641 gcggccacaa tggttacggt gactcccgtc cctgtcaact caatacgaag cgactcaaaa
     2701 aatccgttca tcgcatgctt actggcacaa taggctgaat gagcaggcac tcccgtcaaa
     2761 cttgcgacgc tggagatcgc aacaatccgg ccttgcgtgg cctttaagaa cggcaacgcc
     2821 aatttggtac agtagacgct gccgaaaaaa
//
LOCUS       DATIAL010000122         2551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_451544, whole genome shotgun sequence.
ACCESSION   DATIAL010000122 DATIAL010000000
VERSION     DATIAL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451544"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VLA60_03895"
     CDS             complement(<1..422)
                     /locus_tag="VLA60_03895"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSF08531.1"
                     /translation="MASFHSPEPADSLSKPTTMYPPQPDTFDELVASDGSVRPHWQNL
                     LKQFESLDATQRRLARETAARMLKDDGMTYLASQSERQRDRPWQLDLFPLLISQEEWQ
                     HLEAGLIQRARLLNHILSDLYGPQQLLKNGTLPPAVVFG"
     gene            complement(739..>2551)
                     /locus_tag="VLA60_03900"
     CDS             complement(739..>2551)
                     /locus_tag="VLA60_03900"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSF08532.1"
                     /translation="GPRRWVTAAWTTRSQHLFLVPGDSPIGFRLPLPSLPVIDPKDYP
                     YIIPTDPFDIRGPLPDPIQESTLQEHRLQKLQGEDGQHEPAQVIMGQKSGIGAGGVVR
                     ASLTVEPRNGHLCVFMPPLESAEDYLDLVAVIKDTAAELNQPIHLEGYTPPYDPRINV
                     LKITPDPGVLEVNIHPAKNWADMVAITTAIYEEARLCRLGTEKFMLDGRHTGTGGGNH
                     VVMGGSTPADSPFLRRPDLLRSIIAYWQRHPSLSYVFSGLFIGPTSQAPRIDEARHDS
                     LYELELGFAQVPAPNVGAPPPPWLVDRIFRNLLIDVTGNTHRTEICIDKLYSPDGPTG
                     RLGLVEFRAFEMPPHERMSLAQQVLLLALVARFWKTPDQGNLVRWGTQLHDRFMLPHF
                     LAQDLHEVIQEMNHAGYELQDDWFAPHLEFRFPLFGSINYGSLVMEVRQALEPWHVMG
                     EQGAPGGTVRYVDSSVERLQIKLSGLTENRYIIACNGKRVPMIPTGRQGEAVGGVRYR
                     AWQPPNCLHPKIGIDAPLTFDIYDQWAGRAVAGCTYHVAHPGGRNFEHFPVNAYEAES
                     RRLARFHSFGHTAGPYAEPPDTSRPEFPCTLDLRWPA"
BASE COUNT          559 a          662 c          750 g          580 t
ORIGIN      
        1 ccgaacacga cagcaggggg taaggttccg tttttcagca attgttgcgg gccatacaaa
       61 tcgctcaaaa tatggttgag gagacgggct cgttgaatca ggcctgcctc aagatgttgc
      121 cattcctcct ggctgattaa cagtgggaat aagtcaagct gccagggtct gtcccgttgt
      181 cgttcactct gactggcaag ataggtcatg ccgtcatctt tcaacattcg tgcggcggtt
      241 tcgcgagcca aacgccgttg agtagcgtcc aggctctcga attgtttcaa cagattttgc
      301 caatgcggac gaacagagcc gtccgatgcc acgagttcat caaaggtatc cggttgaggc
      361 gggtacatcg tggtgggttt ggaaagggag tccgctggtt cgggagaatg gaaggacgcc
      421 atggaattta tcaggggaat gttgaaaaag gccgccagcc gcgttccctg ccttcgccgg
      481 agcggcttcg cacaggcagg tcgtgcttgg ccgtgctcac gtactcctcc gtacgctccg
      541 cttgcccaag cggctgcggc cttgctggac gagccttttt gaacactccc ttattttttc
      601 catgcgggtt tctgccaatc atatgctgct gaaaaggcaa atattgaaaa aaatcaagag
      661 tcccatcagg gtgtttttcg ttgagggatg gtcaaaatca ccttaccgtt tcctggatac
      721 actgtccact gtatctgatt atgccggcca tcggaggtca agtgtgcaag gaaattccgg
      781 gcgggaggta tctggtggtt cggcataggg tccggcggta tgaccaaagg aatgaaacct
      841 ggccaaccga cggctttcgg cttcataggc gttgacggga aaatgttcaa aattacgtcc
      901 gcccgggtgg gccacatgat aggtacaccc ggcaaccgcc cgcccggccc attgatcgta
      961 aatatcgaaa gtcagcggcg cgtcaattcc gatcttcgga tgcaaacagt ttggaggctg
     1021 ccaggcccgg tatcgcacgc cgccgaccgc ctctccttgt cgtccggttg gtatcatggg
     1081 cacgcgtttg ccattgcagg caataatata acggttttca gttaagccgg acagtttgat
     1141 ctgtaggcgt tcgaccgatg aatccacata tcgtacggtg ccgccaggtg ccccttgctc
     1201 tcccatgaca tgccagggtt ccagagcctg tcgaacttcc atcaccagac tgccatagtt
     1261 gattgagcca aagaggggaa aacggaactc caggtgaggt gcgaaccagt cgtcttgcag
     1321 ttcgtacccg gcgtgattca tttcctggat gacttcatgg agatcctgag cgagaaagtg
     1381 ggggagcata aaacgatcat ggagttgggt cccccatcga acgaggtttc cctggtccgg
     1441 agttttccag aaacgcgcaa cgagggccag gagcagaacc tgttgagcca gactcatgcg
     1501 ttcgtggggc ggcatttcga atgcccggaa ttctaccagt ccgagtcgac cggtcgggcc
     1561 atccggggaa tagagtttgt cgatacaaat ttccgtgcga tgcgtattcc ccgtgacatc
     1621 aatgagcaaa tttcgaaaga tccgatcgac caaccatggc gggggtggcg ccccgacgtt
     1681 gggagcgggg acctgggcaa atcccagttc cagttcatac aaggaatcat gacgggcttc
     1741 atcgatgcgg ggagcctgac tggtcgggcc gatgaagaga ccggaaaaca cataggagag
     1801 ggaaggatgc cgttgccagt aggcgattat gctgcgcagg agatcgggcc gacgcagaaa
     1861 gggactgtcg gccggggtgc tgcctcccat gaccacatga ttgcctcctc cggttcccgt
     1921 gtgccgccca tccaacatga acttttccgt gcctaatcga cagagcctcg cttcttcata
     1981 gatggccgtg gtaatggcaa ccatgtccgc ccaatttttc gcggggtgga tattcacctc
     2041 gagcactcca gggtcgggag tgattttcaa gacgttgatg cgagggtcat aggggggtgt
     2101 gtagccttcc aagtgaatcg gctggttcaa ttccgcagcg gtgtccttaa tgacggcgac
     2161 cagatcaaga taatcttctg ccgattccag gggcggcatg aatacgcaga ggtgcccgtt
     2221 tcgaggttcc acggtgagag atgcgcgaac cacacctcct gcgccgatcc cgctcttctg
     2281 tcccataatc acttgagccg gttcatgttg cccgtcttct ccctgtagtt tttgcagccg
     2341 gtgttcctga agggtggatt cctgaatcgg atcgggtaac ggtccgcgga tgtcgaatgg
     2401 atcggtcgga ataatatagg gatagtcttt gggatcaata accggtaatg atggcagggg
     2461 caatcgaaaa ccgatcggcg agtcacccgg gaccaaaaac aggtgctgac tgcgtgtggt
     2521 ccacgccgcg gtgacccagc gacgtggtcc a
//
LOCUS       DATIAL010000123         7173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_21913, whole genome shotgun sequence.
ACCESSION   DATIAL010000123 DATIAL010000000
VERSION     DATIAL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21913"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..1197)
                     /locus_tag="VLA60_03905"
     CDS             complement(403..1197)
                     /locus_tag="VLA60_03905"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HSF08533.1"
                     /translation="MTTLNHIDEQNLALAVDLLENPSFAAKVTNLIGGPVERAITMLP
                     EGFSNKLLTTTQKALSKALDLAVSSIDERYRGEPANGAHRVLTTVSGAIGGAMGLMAL
                     SVELPISTTLMLRSIADIARSEGEPIMEAGSKLACLEVFAMGGTSKADDGAETGYFAV
                     RAALAGAMTEAGKYLLERGFTRSGAPALVRFLSEIASRYSIQVSEKAAAQAIPLLGAA
                     GGATINLLFMDHFQNMARGHFIVRRLERTHGKEAVREAYRQFAARN"
     gene            complement(1306..2406)
                     /gene="ribD"
                     /locus_tag="VLA60_03910"
     CDS             complement(1306..2406)
                     /gene="ribD"
                     /locus_tag="VLA60_03910"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HSF08534.1"
                     /translation="MKRALSLASKGKGRTSPNPMVGAVIVKDGQIVGEAYHRQSGEPH
                     AEILALHQAGPRARGAVLYVTLEPCCHTNKRTPPCVPLLIQSGLARICVAMVDPNPQV
                     NGSGLQQLKQANVPVSVGVLEQAARQLNKVYAYWITTGRPLVTLKGAMTLDGKIATAT
                     GESRWITGERARQDVHRLRNQMDAILVGVGTVIADDPELSARGTTMTNRRVGRQPVRV
                     VLDSRLRIPSNAKVLQWVGEQPTILCTTTQAPRQRIAHLRKRGIQVWVLPGQGGRVSM
                     KACLSRLGKEGLTSVLIEGGGRVNAAAFQQGLVNQVRLYMTPKLLGGQDAIGLIGGQS
                     PKTLKRAWPLADCQLNKLGNDWLVTGTIEPRR"
     gene            complement(2433..5534)
                     /gene="ppdK"
                     /locus_tag="VLA60_03915"
     CDS             complement(2433..5534)
                     /gene="ppdK"
                     /locus_tag="VLA60_03915"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HSF08535.1"
                     /translation="MRHKKYVYFFGGGKAEGSGNMKELLGGKGSGLAEMTNLKISVPS
                     GFTITTEACVEYFHAKKRFPAGMWDQALNGLRQVEKTMKARLGDPDNPLLVSVRSGAR
                     ASMPGMMDTVLNLGLNQQTVQGLGKKTGNRRFAVDAYRRFITMFGSVVMGIPRERFEH
                     TLEAMKQAQGVKHDTDLTEDALAQLVVQYQELVKTETARDFPQDPFEQLRMAVDAVFD
                     SWFGDRAVTYRRLYDIPDEWGTAVNVQSMVFGNMGDTSGTGVAFTRNPANGAPVFFGE
                     CLLNAQGEDVVAGIRTPLPVVALQDTLPEAYKDLIKTQRTLEKHYRDMLDLEFTIQEG
                     KLYMLQTRIGKRTGIAAVRIAVDMVRQGLIDRREAVKRVAPEQLSQYLYPIFESSDEA
                     KFQSVGKGLPAGPGAAAGRIALTPDQAVAMKAKGERTILVRHETSPDDIHGMHAAEGF
                     LTAKGGMTSHAAVVARQMGKVCIAGCDAVEVLSNGTVQFGSMILSEGDFLSLNGFTGQ
                     VYAGDIPVVDSEVIQVIQGRMDQKDSEKYQYFSTILKWADAFRVLKIRSNADIPEQAH
                     IARGFGAEGIGLCRTEHMFFAEDRVPIMQQMILASSREERELYLEQLLPLQRQDFIGL
                     YREMKGFPVTIRLLDPPLHEFLPKREHLLVELAKLEVTNGDPQVIQEKQRILDRVEEL
                     HEYNPMLGLRGCRLGITMPEITRMQVQAIFEAACEVAREGKKIVPEIMIPLVGMASEM
                     KAQKELIQEVAQQTMAEYGMTLNYLIGTMIELPRAAVTAGRIAEHAEFFSFGTNDLTQ
                     TTFGFSRDDAGPYIGFYMDRQDRCPVCQRTNVDWKKMVCRMCKATIDKKAENILDADP
                     FAVLDQEGVGALMKWAIQEGRKTRPLIKLGICGEHGGEPSSVAFCHELGLDYVSCSPY
                     RVAIARLAAAQAAVTVLEREESQRQPRSTPSKSGKSRTVATKSGPTKTTKKAPSTKTT
                     GKKVTTRKVVAGKKPATKKPAAKSAVKKNVVTKAKSKKSPSKKMASSSPTRKKRATAA
                     KRR"
     gene            complement(5756..6241)
                     /locus_tag="VLA60_03920"
     CDS             complement(5756..6241)
                     /locus_tag="VLA60_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08536.1"
                     /translation="MGDGTDRVESPEISRRLKRFTLVVTVALIIGCNALILTGLWVSG
                     VDLEKIFSKSELYDPVNGHCVQVTWLKVVGADGLVKVCSEWLDFSDISGETHLLPEGK
                     TLAMGPDGNLYVSGNPAENYRLIALMIFAIVVIISGMWTKQMLIAKYQDRLQSVNHHS
                     T"
     gene            complement(6311..>7173)
                     /locus_tag="VLA60_03925"
     CDS             complement(6311..>7173)
                     /locus_tag="VLA60_03925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSF08537.1"
                     /translation="VYARHDGESAEVCRALGEQYFPRFPEDQLPTGLPGVLLAVADRC
                     DSIVAFFAAGMSPSGSEDPLGLRRASYGLVRIIAETPLRLDITSVVNRALQILDGQGG
                     RVRGTPQTGSEVVAFILDRLRFYGRQHLGLREDVMEAVIRIPHAMDCDMGDLVARMQA
                     LEAMVPQPDFDPLMIGFKRAHRIVEKEQWTNAQALPEQFVHESERRLFQALGVIQQQV
                     RSSVNDRNYGKALHSLLELKGPIDEFFGAVMVNDSDPQIRANRLSLLKATDDLFLTVA
                     DLSCLQSASG"
BASE COUNT         1591 a         2117 c         1726 g         1739 t
ORIGIN      
        1 ggatcatacg gaaataatgg ttcgatccct tcgacgttgc tcgcaggaca aacccttcgg
       61 ccaagtgcag aacaggctct agaagatttc gcacctctcc ttctcatcct aagagcaacg
      121 aaggatctga gcccagccca agccggccat ggcttagcca aatgcttcgc gctgctcagc
      181 atgacaattc ggttgaggat gtctcccacc tggtcttctt gccattctca gacgatcaat
      241 gcaccccgtc tagatccatc ggttctgatt aataagcgct ataggccgtg gaaccctcta
      301 ttctgtatga ttcccaattt ccccagtgcg atcttcctgg aatgcaatca gatggatatc
      361 cgcgagcgac tgactgggat gacaaaccgg aaagcgacga atctaattcc tggcggcaaa
      421 ttggcgatag gcctcccgca cggcctcttt gccatgcgtc cgttccaatc gccggacaat
      481 aaaatggccg cgcgccatat tttgaaaatg atccataaac agcagattaa tggtggctcc
      541 ccctgcggcc cctaacaacg ggatcgcctg ggcggctgct ttttctgata cttgaatact
      601 gtatcgtgag gcaatttccg acagaaaccg gaccaatgcc ggggcacctg acctcgtgaa
      661 accacgttcg agcagatact tgcccgcttc ggtcatcgca cctgccaacg cggccctcac
      721 cgcaaaatat cctgtttccg ctccgtcatc cgctttactg gtgcctccca tggcgaacac
      781 ctccaaacag gccagtttgc tcccagcctc cataatgggt tctccctcac ttctggcaat
      841 atcggcgata gatcgcaaca tcagcgtcgt agagatcggc aattctacac tcaatgccat
      901 gagccccatc gctcccccga ttgcaccact gacggtcgtc aatactcggt gagccccatt
      961 ggcgggttca ccccgatacc gttcatctat actggagacg gccagatcta acgctttgct
     1021 caaggctttt tgggtggtcg tcaggagttt attggaaaat ccctcgggca gcatggtgat
     1081 ggccctttcg accggccctc cgatgagatt tgtgactttg gcggcaaagc tggggttttc
     1141 taagagatct acggctaatg ctaaattctg ctcgtcgatg tgattgagag tggtcatgag
     1201 cgcaatctcc cctgttgttc tgttcaccac acgattggcg ttttatctct tgtaatggtt
     1261 agcagacacc atcaccttgc ttattgtcga tgcctaagta gaccctcacc gtcgtggctc
     1321 aatcgttccc gtgacgagcc aatcattccc gagtttgttg agttggcaat cagcaagtgg
     1381 ccaggcccgc ttcagcgttt tcggggactg tcccccgatg aggccgatgg catcctgccc
     1441 cccgagcagc tttggagtca tatacaatct tacctgattc accaggccct gttgaaacgc
     1501 cgcagcattc accctacctc ctccctcgat gagcaccgag gtgagaccct cttttcctaa
     1561 tcgggataga caggccttca tggaaactct tccgccttgg ccaggcaata cccacacctg
     1621 aataccacgt tttctcaaat gtgcgattct ttggcgtgga gcctgtgtgg tcgtgcacaa
     1681 aatggtgggt tgctcaccca cccattgcaa aaccttggca ttggagggaa tgcgcaatcg
     1741 gctatcgaga acgactctta ccggttgccg tcccacacgt ctgtttgtca tcgttgttcc
     1801 tctagccgac aattcaggat cgtcggcaat gaccgttccg acgccaacca gaattgcatc
     1861 catctggtta cgcaaacgat ggacatcctg acgtgcacgt tcccctgtga tccaccgtga
     1921 ctcaccggtg gccgtcgcaa tttttccatc caaggtcatt gcccctttga gagtcaccaa
     1981 cgggcgtccg gtcgtgatcc aataggcata aaccttattc aattgccggg cagcttgttc
     2041 taatacccca accgataccg gaacattggc ttgcttgagt tgttgaagcc cgctcccatt
     2101 gacctgtgga ttgggatcga ccattgccac gcatattcgg gctaacccgg actgaatgag
     2161 gagtgggaca cagggaggcg tgcgcttgtt ggtatgacaa cagggctcta acgtcacata
     2221 gagaacggcc cctcgcgctc gagggcccgc ttgatggagc gctaaaattt ctgcatgagg
     2281 ttcgccggac tgacggtgat aggcttctcc gacgatctgc ccatccttga caatcacggc
     2341 cccaaccatg ggattgggac tcgtacgccc tttccccttg gaggcgaggg aaagggctcg
     2401 cttcatgtag gtgtcatctg atgggacgga cgtcaccgcc tttttgcagc cgtagcccgt
     2461 tttttacgtg taggagaaga agaagccatt tttttggatg gactcttttt gctttttgcc
     2521 ttcgtcacga cattcttctt cactgcgctc ttcgctgccg gtttctttgt ggccggtttc
     2581 ttcccggcca cgaccttcct ggtcgtgact tttttcccgg tcgtttttgt ggatggcgcc
     2641 tttttagtcg ttttggtagg accggatttc gtcgccaccg ttcgggactt accgctcttg
     2701 ctcggcgttg atcgaggctg tcgttgactc tcctcccgtt cgagcaccgt cacggcggct
     2761 tgagccgcag cgagccgcgc gatagccaca cggtagggcg aacagctcac ataatccagg
     2821 cctaattcat gacaaaaggc cacggaactg ggctcaccac catgctctcc gcagattcca
     2881 agtttgatta acggtcgcgt cttccgccct tcctgaatcg cccatttcat gagggctcca
     2941 accccttctt gatccaatac ggcaaaggga tctgcgtcca aaatattttc ggcctttttg
     3001 tcgatcgtgg ccttgcacat ccggcacacc attttcttcc aatccacgtt ggtccgttga
     3061 cagaccgggc accggtcctg gcgatccatg taaaacccga tatagggtcc tgcgtcatcg
     3121 cgagaaaacc cgaacgtcgt ttgcgtcaga tcattggtcc caaaggaaaa gaattccgca
     3181 tgttcggcga ttcgccctgc cgtcaccgcc gcgcgaggga gttcgatcat cgtgccgatc
     3241 aagtaattca gcgtcatgcc atattccgcc atggtctgtt gtgccacttc ttgaatcagt
     3301 tctttttgag ctttcatctc cgacgccatc cccaccaacg ggatcatgat ctccggcacg
     3361 atctttttcc cttcgcgcgc gacttcacac gccgcttcaa aaatcgcctg aacctgcatg
     3421 cgcgtaattt ccggcatggt tattccaagc cggcatccac gaagacccag catgggatta
     3481 tactcatgca actcttcgac acgatccaat atccgttgtt tctcctgaat gacctgagga
     3541 tctccattgg tcacctccaa cttcgcaagt tccaccagca ggtgctcgcg tttgggcaaa
     3601 aattcatgga ggggaggatc gagtaggcga atcgtgaccg gaaatccctt catttcacga
     3661 tagagtccaa taaagtcctg tcgttgaagt ggcaacagct gctctaaata caactcccgc
     3721 tcttctctgg aggatgccag gatcatttgc tgcatgatgg gtacgcggtc ttcggcaaaa
     3781 aacatgtgct cggtccggca gagcccgata ccttcggccc caaacccacg tgcgatgtgc
     3841 gcttgctcgg gaatatccgc attcgatcga atcttcagca ctctgaacgc atcggcccat
     3901 ttcagaatcg tcgagaaata ctgatacttt tcagaatctt tctggtccat tcttccctga
     3961 ataacttgga tcacttcgga atccaccacg ggaatgtctc cggcatagac ctgtccggta
     4021 aacccgttca aggagagaaa gtctccttca gagagaatca tggatccaaa ttgcaccgtt
     4081 ccgttcgaga ggacttccac ggcatcacag ccggcaatac agacttttcc catctgacgg
     4141 gccactacag ccgcatgcga ggtcatcccg cccttggccg tcagaaatcc ttccgcggca
     4201 tgcatgccat gaatatcatc cggactcgtt tcatgacgga ccaggatggt ccgttccccc
     4261 ttggctttca tggccacggc ttgatccggg gtaagagcaa tgcggccggc cgcagctcca
     4321 ggacccgctg gaagcccttt accgactgac tgaaatttcg cctcatcact gctttcgaaa
     4381 atcgggtaca gatattggga aagttgttcc ggggcaaccc gcttgacggc ttctctccgg
     4441 tcgatgagcc cctgacgaac catatctacg gcaatccgaa ccgcagcaat acccgtccgt
     4501 tttccaatcc gggtttgcag catatacaat ttgccctctt ggatcgtgaa ttccagatcg
     4561 agcatgtccc ggtaatgttt ttccagggta cgctgggtct tgatgagatc cttataggcc
     4621 tccggcaacg tgtcctgtaa cgcgacgacc ggcagggggg ttcgaatacc tgccacgaca
     4681 tcctcgccct gggcattcaa cagacactcg ccaaaaaaga ccggggcgcc gttcgcgggg
     4741 tttcgggtaa aggctacccc tgtcccgctg gtgtcgccca tgttgccgaa caccatcgac
     4801 tggacattca cggccgtccc ccattcatcg ggaatatcgt aaagccggcg atacgtcacg
     4861 gcacgatccc caaaccagga atcaaacacc gcatccaccg ccatccgaag ttgttcaaat
     4921 ggatcttgcg gaaaatctcg cgcggtttcg gttttgacca attcctgata ctgcaccact
     4981 aactgcgcca aggcgtcttc ggtcaaatcg gtatcgtgct tgaccccttg tgcttgcttc
     5041 atggcctcca gggtatgttc aaatcgctca cggggaatgc ccatcaccac gctgccaaac
     5101 atggtgataa accgccgata ggcatcaacg gcaaaccggc gattgccggt ttttttgcct
     5161 aaaccctgaa ccgtttgttg gttgaggccg agattcaata ccgtatccat catacccggc
     5221 atcgacgctc gtgcccctga ccgtaccgac accagcaacg gattgtccgg atcgccgagt
     5281 ctggccttca tggttttttc gacctgccgc agaccattca gcgcttgatc ccacatgccg
     5341 gcgggaaagc gttttttggc atgaaagtat tccacacagg cttcagtggt aatcgtgaat
     5401 cctgaaggaa cagaaatttt caggttggtc atttcagcca atcccgaccc tttccctcct
     5461 aagagttcct tcatgttgcc ggacccttcg gctttgccgc cgccaaagaa atagacatat
     5521 tttttgtgcc gcatttcttc gctctccttt acccgaccca tcggcccttc gaaaaagaag
     5581 acgtcgtcac gggaggagac tgaatgtctg acattcttct cccaaccatt ctccgcgctg
     5641 aatggagacc ctcacgggca acagtctttt ctctcaaaga tgcctgagac aggcgtggtg
     5701 tttgaatatg aatatatgag ctggctgtca gaacaaaggt aggatacacg agcattcagg
     5761 ttgaatgatg gtttacagat tgaagacggt cctgatattt ggctatcagc atctgcttgg
     5821 tccacatgcc ggaaattatg acaactatgg caaaaatcat gagcgcaatc aatcggtaat
     5881 tctcggctgg gttcccggag acatacaggt tcccatccgg ccccatagcc agggtttttc
     5941 cttccggcaa cagatgcgtt tcacccgaga tatcggaaaa atcaagccat tcggagcaga
     6001 ccttcaccag cccatccgcg cccacaacct ttaaccaggt gacctggaca caatgcccat
     6061 tcacgggatc gtagagttcc gattttgaga aaattttttc caaatcaact ccgctcaccc
     6121 aaagacctgt caggattaag gcattgcagc cgataataag ggccaccgtg acaaccagag
     6181 taaagcgctt cagccggcgt gaaatctcgg ggctctctac acggtccgta ccatccccca
     6241 tcggatgttc cttcccatga ccgtctcgct ttcacaaggg cggcccggaa tcttcttcga
     6301 ttaacgactg ttatccactt gccgattgca gacaggacaa atcggctacc gtgagaaaca
     6361 ggtcatccgt cgcctttaac agggaaaggc gattggcccg tatctgtggg tctgaatcat
     6421 tgaccatcac ggctccgaag aactcatcaa ttgggccctt gagttccaac aacgagtgaa
     6481 gagcctttcc gtaattccgg tcattcactg aactgcggac ttgttgttgt atcaccccca
     6541 aggcctgaaa cagccgacgc tccgattcat gcacaaattg ttcaggcagg gcctgcgcgt
     6601 tggtccattg ctctttttcg acaattcggt gagcccgctt aaatccgatc atcagcggat
     6661 caaaatccgg ttgaggcacc atggcctcca aggcctgcat gcgtgcgacc agatcaccca
     6721 tgtcacagtc catcgcatga ggaatccgga tgaccgcttc catgacatct tcccgtaatc
     6781 ccaggtgttg cctgccataa aatcgaagcc ggtcgagaat aaatgccacc acttctgacc
     6841 cggtctgagg agtgccacgc acccgcccac cttgtccatc caagatctgc aacgcccgat
     6901 ttaccacgga ggtgatgtcc agccggagtg gcgtttctgc aattattcgc actaacccat
     6961 acgaggctcg acgtaacccc aacggatctt ctgagccgga gggggacatt ccggccgcaa
     7021 aaaaggccac gatggaatca caccgatcgg ccaccgcgag taggacaccc ggtagtccgg
     7081 tcggcaactg atcttctgga aatcggggaa aatactgctc gccaagagcg cgacagacct
     7141 ctgcagattc gccatcgtgt cgggcatata ctt
//
LOCUS       DATIAL010000124         5315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_774983, whole genome shotgun sequence.
ACCESSION   DATIAL010000124 DATIAL010000000
VERSION     DATIAL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774983"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VLA60_03930"
     CDS             <1..427
                     /locus_tag="VLA60_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF08538.1"
                     /translation="GSTDFYNMGGGLVLSYPIGNRSAQSQYQRRILETQQSQVSLLRV
                     RQQIILDVKEAIRQIQTSFKRTRTTQTARQLSERQLNAEQERLNLGLSTTRLVLEFQR
                     DLRIARGRELRAILDYNQSLSRLRLVTASTLDHYHIEIQ"
     gene            736..1875
                     /locus_tag="VLA60_03935"
     CDS             736..1875
                     /locus_tag="VLA60_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF08539.1"
                     /translation="MNKTSWILTIIILAGAVGGWVYYSRSTGQPSPETAHHNVVEVVR
                     TSLQTKVSETGTIMPSQTIEIKSQFSGEVAQLFVTAGQEVQKDQVLAIIRQESSQARQ
                     VAQLRAGISEERLNVDTAHREWIRAESLFRKGFIPQKELELSHRDYQQSLVRLELAER
                     QLLLALGGNKELYERYRDGSASKTRPEEFQVRSPSQGTVLEILVHTGEIITSGTATFG
                     GGTILMRIADLSHMVVKAKINEVNIPRVSVGQSVDIRLDALPEKLFTGRVMAIAAQGV
                     KENNLVTYEVSIDIENSHSDLKPMLTANIDIETKRLDNVLTVPLDVLRVNKGDDVVDV
                     LVEGQPQTRKVRVAFRTDTQAIITQGLQEHDQVVVPSFKAAEARR"
     gene            1896..2597
                     /locus_tag="VLA60_03940"
     CDS             1896..2597
                     /locus_tag="VLA60_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF08540.1"
                     /translation="MLIELNHIFKIYQAGHVEVPALIDITLQVNKGEFVAIIGQSGSG
                     KTTLLDILGCLSRPTSGEYWLDGDLVNTLPDSRLTGIRNKKIGFIFQTFHLLARKTAL
                     ENVQLPLMYAGASRTVQHDTARTLLTKVGLQDRLRHYSNQLSGGQQQRVAIARALANS
                     PALLLADEPTGNLDSQSGKDILALFEQLHRHGQTIVMITHDPLIAQQAERRITLADGR
                     VVADEYMHHPLPVPG"
     gene            2602..3822
                     /locus_tag="VLA60_03945"
     CDS             2602..3822
                     /locus_tag="VLA60_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF08541.1"
                     /translation="MLRSIFLDALKNLSGNALRSGLTMLGVIIGVAAVITMIAIVEGG
                     QVWLVNSLERMGTNLLFVWKKRLTVEERQLFAGRNTELRYDDALAIQTRFPDLLVAPI
                     IELDGQLKAGDRDYSGRITGTSPVYSQIRNFRPESGRFVSSIDIKEWNRSVVLGHTIA
                     EVLFGSQPAIGEDVKIGDQRFTVKGIMEPKGEIYGHDYDEMVIIPISTGLRFFQGSDK
                     IRSMIIHVPDRQRMDEISQALHQFLVQRHEGVDDIHIRNQGEFLSAVDQTLLTFRLVL
                     GGIAVIALLVGGIGIMNIMLVTVTERTREIGLRKAIGARRQDILLQFLIESTTISVIG
                     GSIGILVGILAAYGIGDVVARAMPGGGDWGAVVQPTAIAIAFAFAVVVGVGFGLFPAM
                     KASKLEPAEALRYQ"
     gene            3886..4581
                     /locus_tag="VLA60_03950"
     CDS             3886..4581
                     /locus_tag="VLA60_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08542.1"
                     /translation="MIPQKERAAGLAFLPIFTTIGFYLLPTNWQLHTAVQFVPQLMAY
                     AALSAWAYKNGDLRHKLGLGFCNIQAGVTRGTAVGLTLGILNTGIILYVMPAWGVDIN
                     FLSQTPHAQIPFWIMMPWFIILIAMGVELNFRGFLLGRLLVWFQHVRQPGHPMTTRIR
                     LQSFLPLSLSALTFAFDPFMVSTFRDLHWIAVWDGLVWGWLWMRMHNLYTVIAAHAVE
                     VILLYLIIRGILT"
     gene            4578..>5315
                     /locus_tag="VLA60_03955"
     CDS             4578..>5315
                     /locus_tag="VLA60_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08543.1"
                     /translation="MTPSFSPHLVNHPFGDPGLYVDIRWSRRALLFDLGDNVSLSPTQ
                     LLRAQDIFISHTHMDHFIGFDRLIRVALGRGKHLRLHGPPGLIENVQGKLRGYTWNLV
                     DGYPLTIEAREYHSTYIQPAQFHAKDAFACKRETVIPGDHSRIFPVLQEPALLVKAVA
                     LNHRIPSLAFSLEEPFHINIKKARLHEAGLPVGSWLKEVKQLILDGAPDSHIFSATIY
                     VEHRKEERVFDVGTMKREFTTITKGQKI"
BASE COUNT         1296 a         1438 c         1298 g         1283 t
ORIGIN      
        1 aggcagcacg gatttttata atatgggagg aggactcgtc cttagctacc ccataggaaa
       61 ccgctcagcg cagagccaat atcaacgtcg gatccttgaa actcaacaaa gccaggtctc
      121 tcttctacgt gttcgccaac aaattattct ggacgtcaaa gaagccatcc gtcagataca
      181 gacaagcttc aagcgcacca ggacgactca aacggcgcgc caattgtccg aaaggcaact
      241 caatgcggag caggaacgcc tcaatctcgg tctcagcacc actcgattag ttttagaatt
      301 tcaaagagat cttcgaatag ctcggggtag ggaattacga gccattctgg actacaacca
      361 atcattatcc cgcctccgcc tcgtcaccgc atcgacactc gatcattacc acattgaaat
      421 tcagtaaaga aaaaaatgaa aaatccccgt aaagattttc tccccgcccc gagaatgttc
      481 catgtgggtt gataccacac cattttcatt tcgggaggcc ttgccaaaat gaccatgccg
      541 gtcctcctgc ccgaagcgaa gtaagcttcc ggcaaagggg ccttgttacg aacctctcct
      601 ttttactccg gtttgtctga attcaatcaa acccctatga gcaaagcgga caaccctctc
      661 atcccgcaat ttatcccttt cctgtctttc tatttcccag gcctttgcta gacttgttac
      721 catacaaata aagatatgaa caaaacatct tggattttga ccatcatcat acttgcggga
      781 gcggtgggtg gttgggtgta ttattcccgg tcgactggtc aaccttctcc tgaaaccgca
      841 catcacaacg tggtcgaagt tgtccgaacc tcgcttcaaa cgaaggtgtc ggagacgggt
      901 acgatcatgc cttcccaaac catcgaaatt aaatctcaat tttccggcga agtcgctcaa
      961 ttgtttgtga ctgcggggca agaagttcaa aaggatcagg tcttagcgat tattcggcag
     1021 gaatccagcc aggcgcgcca ggtggcccaa ctcagagcag gtatctcgga agaacgctta
     1081 aacgtggata ccgctcatcg ggaatggata cgtgccgaat ccctcttcag aaaagggttt
     1141 atccctcaaa aagaattgga actttcccat cgggactatc aacaatccct ggttcgtctg
     1201 gagttggccg agcgacaact attattggca ttaggcggca acaaggaatt gtatgaacga
     1261 taccgtgatg gctctgcctc caaaacccgc cctgaagaat ttcaggtccg gtcaccctcc
     1321 caaggtaccg tcctggaaat tcttgtccat accggagaaa tcattacgtc cggaaccgcc
     1381 acattcggcg gcggaacaat cctcatgcgt atcgccgatc tgtcccacat ggtggtcaaa
     1441 gccaaaatca atgaagtcaa tattccaagg gtttcggttg gccagtcggt ggacattcgc
     1501 ctggatgcct tacccgaaaa actttttacg ggccgggtga tggccattgc cgcccagggg
     1561 gtcaaagaaa acaatctggt gacgtatgaa gtctctatcg atatagaaaa ttcccactcc
     1621 gatttaaaac cgatgctgac ggcaaacatc gatattgaga caaaacggct ggataatgtc
     1681 ctcaccgttc cgcttgacgt cttacgtgtc aacaaagggg atgatgtggt ggacgtgctc
     1741 gtggagggac aaccccagac ccggaaggtc cgtgtcgcct tccggactga tacgcaggcc
     1801 atcatcactc aaggacttca ggaacacgac caggtcgtcg ttccatcctt taaagccgca
     1861 gaggctcgcc gataacgccg gaagtagatt tggccatgtt gattgagctc aaccacatct
     1921 tcaaaatcta ccaagcgggg catgtcgagg tccccgcctt aatcgatatc actctccagg
     1981 taaataaggg agaatttgta gccataatcg gacagtccgg aagcgggaaa accaccctcc
     2041 tggatattct tggctgtttg agccgcccga cgagtggcga gtattggctg gacggggatc
     2101 tggtcaacac cctccccgac tcccgcttaa ccggtatccg taacaaaaaa atcgggttta
     2161 tctttcaaac cttccattta cttgcgcgaa agaccgccct ggaaaatgtc caacttcctc
     2221 tgatgtatgc gggcgcctca cgaacagtcc aacacgacac cgcacggaca ttattaacca
     2281 aggtcggcct gcaagaccgc ctgcgccatt acagcaatca gttatcagga ggccaacagc
     2341 aacgcgtggc gatcgcgcgt gcgttagcca acagtccagc gctcctcctg gcggacgaac
     2401 ccacgggaaa cctggatagt caatctggca aagacattct tgcgctcttt gaacaactgc
     2461 atcggcatgg acaaaccatc gtgatgatta cccacgatcc cctcatcgca cagcaggcgg
     2521 aacgccgcat caccctggca gatggcagag tcgtggccga tgaatacatg catcaccccc
     2581 tcccggtgcc gggataaaat catgttacgt tcaatttttc tggacgcgct caaaaatctc
     2641 tcagggaacg cgcttcgttc ggggcttacc atgttgggag tcattattgg cgtcgccgcg
     2701 gtcattacca tgattgccat tgtggaaggc ggacaagtct ggctggtgaa ttcgcttgag
     2761 cgcatgggca ccaatttgct gtttgtctgg aaaaagcggt tgaccgttga agaacggcag
     2821 ctctttgcgg ggagaaatac ggaactccgc tatgacgacg ccctggccat tcaaacacgt
     2881 tttcccgatc tgcttgttgc tcccattatt gagctggatg gtcaattaaa agcgggggat
     2941 cgtgactaca gcggacgaat caccggcacc tctcccgtat atagccagat ccgaaacttt
     3001 cgccccgaat ctggacgatt tgtttcttcc atcgacatca aggaatggaa ccggtcagtg
     3061 gtattagggc acaccattgc cgaagtttta ttcggatccc aacccgccat cggagaggac
     3121 gtaaaaatcg gcgatcaacg atttacggta aagggcatca tggagcccaa aggggagatt
     3181 tacgggcacg attacgacga aatggtgatc attcctatca gcacgggcct gcggtttttc
     3241 cagggatccg acaaaatccg atcgatgatc attcatgttc ctgaccggca gcgcatggat
     3301 gaaatctccc aggcactgca tcaatttctg gttcaacgtc acgaaggggt agacgacatc
     3361 catatccgga atcagggaga atttctgagt gcggtggacc aaacgctttt gaccttccgg
     3421 ctggttctgg gaggcattgc ggtgattgcc ctcctcgtcg gcggcattgg catcatgaac
     3481 atcatgctgg ttaccgtcac cgagcgaacc agggagattg ggctacgaaa agccatcggg
     3541 gccaggcggc aggacattct gttgcaattc ctcattgagt ccacgaccat tagcgtgatt
     3601 ggcggatcca ttggcattct tgtgggcatt ctcgcagctt atggcattgg ggatgtagtg
     3661 gctcgggcca tgccgggcgg aggagattgg ggagcagtgg ttcagccgac cgccattgcc
     3721 attgcgtttg cctttgcagt ggtcgtcgga gtgggatttg gtctctttcc cgccatgaaa
     3781 gcctcgaaac tggagcccgc cgaggccttg cgctatcaat aagtttcagg agtgcgctcc
     3841 tttttacccg gaaccggcca caccgtccat gaccattgcc cagctgtgat acctcagaaa
     3901 gaacgagccg caggattagc cttcctcccg atcttcacca ctatcgggtt ttacctctta
     3961 cccacaaact ggcaactcca cactgccgtc caatttgttc cacaactgat ggcgtatgcc
     4021 gccttaagcg cctgggccta taaaaatggg gatctacggc acaaacttgg gctaggattc
     4081 tgcaacatac aggcaggggt tacccgagga actgcggttg gcctcacgct gggtatactg
     4141 aataccggga tcatcttgta cgtcatgcct gcttggggag tggacatcaa ttttctctct
     4201 cagacccctc atgcccagat accgttttgg atcatgatgc catggttcat catcctgatt
     4261 gctatgggag tggaattgaa tttccgcgga tttctccttg gccgactact ggtatggttt
     4321 caacatgtgc gtcaacccgg tcatcccatg accaccagga ttcgcctaca gtcctttctc
     4381 cccttatccc tatcggctct gacgtttgcc tttgatccct ttatggtgtc gacattccgt
     4441 gatttacatt ggattgccgt atgggatggc ctggtgtggg gatggctatg gatgcgaatg
     4501 cacaatctct atacagtcat agcagctcat gccgtggaag tgattcttct ctatctcatc
     4561 atacggggaa tattgacatg acaccctcgt tttcccctca tctggtgaat catccctttg
     4621 gagaccccgg cctctatgtc gatatacggt ggagccggcg tgccctgctc tttgacctag
     4681 gggataatgt gtccttgagc ccaacccagc ttttacgggc gcaggatatt ttcatctctc
     4741 atacgcatat ggatcacttt atcgggtttg atcggctcat tcgtgtggcc ctgggtcgtg
     4801 gcaaacatct tcgattgcat ggtccaccgg gccttattga aaatgttcaa ggcaaacttc
     4861 gtgggtatac ctggaatctg gttgatggat atccactcac catcgaagcc agagaatatc
     4921 actccaccta tattcaaccc gcgcagtttc atgcgaagga tgccttcgcc tgtaaacggg
     4981 aaaccgtaat cccaggcgat cattcccgaa tttttccggt tcttcaagaa ccggctctcc
     5041 tcgtgaaagc cgtcgcgctc aaccatcgga tcccttctct tgccttttcc ctggaagaac
     5101 cgtttcacat caatattaag aaggcccgac ttcatgaggc aggccttccg gtgggctcct
     5161 ggctcaagga agtcaagcaa ttaatcttag atggcgcccc ggattcacac atcttttccg
     5221 ccacaattta tgttgagcac cgaaaggaag aacgcgtatt tgatgtaggc accatgaagc
     5281 gagaatttac gaccatcacc aaggggcaaa aaatc
//
LOCUS       DATIAL010000125         5913 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_129848, whole genome shotgun sequence.
ACCESSION   DATIAL010000125 DATIAL010000000
VERSION     DATIAL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129848"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(601..1227)
                     /gene="msrA"
                     /locus_tag="VLA60_03960"
     CDS             complement(601..1227)
                     /gene="msrA"
                     /locus_tag="VLA60_03960"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSF08544.1"
                     /translation="MKRIRYFLFSIGLAALSIGGMVALSQESLAAEKTQYAKATFAGG
                     CFWCMEEVYEKVDGVVSVVSGYTGGQLSNPTYEQVSAGGTGHAEAVEVTYDPNQVTYQ
                     HLLEVFWHNVDPTTPNAQFCDHGNQYRTAIFYHDEKQKMLIDESKQAVESSKSFPQPI
                     VTEIIPFSVFFSAEDYHQDFYKKNPVRYKFYKWNCGRSQRLEELWGKP"
     gene            2120..3523
                     /locus_tag="VLA60_03965"
     CDS             2120..3523
                     /locus_tag="VLA60_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08545.1"
                     /translation="MVRTVLRLVMGSLLLPFASGSAFSADTIPHIDRLELRTDSHSNA
                     CDSPNYSSLGRSSPFFSPSTPCTVSKDEYALTRFNFNQKREPFNLQNARDMPSLVNQG
                     GSSLYEASNDIPLFHDALSAGVQMMYRDPFVTDNSATPLTNRMGAQIVLKGTTASMKY
                     QAQYGFSGQETGNTSLTTPNDRVGGKLAWEWNLPFVTPQIELSRFTSNVEGDLSRSQT
                     VANQQKFSLLLSAPNWPSLSLGYARQQKDIFTRPEGPLSNAISTESVSANMSFLHGAG
                     KGDWSSYYKTSQSEYGENGSEEEFGSKLGGTLNLLEPVDLAPQWGFTRRVKSRGTMVN
                     DRYFARLGSNFRVSPTSTLNPGVEFARDLNRFDALRTDTLAAKLGYAYLAVDDSLHVS
                     ILGQYILKQRTNTQANPQIYDISLLFKKDVRDYLHLNHRQQTLSLKIAHNQQMNTSSS
                     QTQSGQTSAMLLVSIIP"
     gene            complement(4050..4838)
                     /locus_tag="VLA60_03970"
     CDS             complement(4050..4838)
                     /locus_tag="VLA60_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08546.1"
                     /translation="MKYLIQRKLRSGRHFFMVCGGVLLGISLCGLPDWGLASDLQIPL
                     HVDSGRLTGQLNQVTLRTVLGQLHKELGLDYVAPDVELEKVISVRLQKEPLHRALSKI
                     LVQWDYAFTLNAAGNITTLHVMAKVSPEVSLSDKKAKGWGPESSEEHANSLQKYGLAD
                     NGIDLVRENQMNKNVLRSNRRTNGKNVSNGQVFAISNVPMEIRPPAPGASMPISAASS
                     KAMLITPGSSGSVMEIIPPTAYPPMNIQPVSKYRQQEMLQPLNP"
     gene            complement(4915..>5913)
                     /locus_tag="VLA60_03975"
     CDS             complement(4915..>5913)
                     /locus_tag="VLA60_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSF08547.1"
                     /translation="TLHLYARAFPAGTITLGGNASGGSSGSMYSVIVQPAESPAPDIT
                     PPTTNVLWRNASSGEVAVWRMNGLTIISVGFPGSTSTEWKIKQVGDINGDGEGDILWQ
                     NTISGVVGIWLMKEGTIQSFGFLGGVSAEWVIKGVGDINGDGKADVIWRNPDSGMVTV
                     WFMDGLSIHSVGLLGGTPKVWEIQQVGDINADGRADLVWQNRTNGTVAVWLMNGLTIT
                     SVGFPASTSLDWEIQLLGDVNGDSKGDVIWRNKKTGMVGVWLMNGATTPLAGFIGGIP
                     LEWEIRQVSDANGDGKGDVILQNRTNGKVAVWLMDGLKIGSVGFPGSTPVNWEIQK"
BASE COUNT         1559 a         1564 c         1244 g         1546 t
ORIGIN      
        1 cttccgttgt taaggcaggt gtcattcaat ctggccttca tccaaaggaa ggaatatatc
       61 agactacaac tgaagatgtc ggttaagatg cggtcatttg ttagtaacgc ggttgaaaga
      121 atcgcaatcc aacagccaaa aggagggaga ggggctaatg cggtcttggg gcattgctca
      181 ttcttatggg caataccatt actgaaggga cggggaattc ataaacacta cgattgtctt
      241 ttttccggac aggaaagatt tggccaaatt tccgaaaact tttcgcacac ctcaccagca
      301 tggataccag gaatagactg gactcttaga ttttgctgca gtgcctcgcc ataggattcc
      361 attatagaac catgaaaaaa tgatcactgg ctgccccatc ctactacccc attgcgcgtg
      421 agcaggcgga agaaaaagaa gccgtccgac gccagcaacg tggatcatgt caatcaagct
      481 cacacggtat gtgccggctg gtaaaggtca agagaaccgg aagaagccta ccccccttac
      541 aaaaccaaga ctataaaata aatgccagaa aggggtgggg tagtaggtcc tattggaaaa
      601 ttatggcttt ccccataact cttccaaacg ctgcgaacgt ccacaattcc atttataaaa
      661 tttgtagcgg acgggattct ttttataaaa atcctgatga tagtcttcgg cagagaagaa
      721 gaccgaaaac ggaataatct cggtcacgat tggttgtgga aatgacttgg aactttccac
      781 tgcctgcttg gattcgtcga ttaacatttt ttgcttttcg tcatggtaga agatcgcggt
      841 tcgatattga ttgccatgat cacagaactg ggcattcggc gtcgtagggt caacattatg
      901 ccaaaacacc tccaacaggt gttgatacgt gacctggttg ggatcgtagg tcacttcaac
      961 ggcttcagca tgcccagttc ctccggccga cacttgctca tatgttgggt tggacaactg
     1021 tccgccagta tacccggaga ctacagatac caccccatca actttttcgt acacctcttc
     1081 catgcaccaa aagcagcctc cggcaaatgt ggctttagca tattgagtct tttctgctgc
     1141 aagactctct tgagacaagg ccaccattcc ccctatcgac agtgctgcca atcctatact
     1201 gaataaaaaa taccgaattc gtttcatcac tacctccatt ttatcaccca ttcgtttaag
     1261 aactcacggg ctttgaggag ttaaaaatat atatcttccc atcaaaatga gcatactcgt
     1321 tagtcccaag aaactgaata tcattacagg acccggttct ttcggaatta caaggctttt
     1381 cataatgccc aaacttctcg ccaaactccc atacttgatg agatgaaaag tggtaaccac
     1441 aggcatccac taattcccaa attcacttgt ggcgtcagac cattttgaac atgctgcggt
     1501 cgttcgtgca taaatatacc tttaggctac ccacatctgt gatgccaagt cctatcccgc
     1561 atctctaatc cctcgaattc tcgccccaac aacaccaaga tattgacccc ttcaaatgat
     1621 ctgccattca gaattataca aatccattgg ttttttgaag cctgctgcgc agattgtcca
     1681 gtgataaaaa gtaattggct atagtcgtca tttttatcgt tttgggcatc ctttatgact
     1741 tttacataca gattttcgat tatttcacga ttatttgcaa aggcgggata gacacctgat
     1801 tcaccgagtt tttggggtgt ccgccatctg cctccacttt atatcgtttc tatttcacca
     1861 caattccttt gtgggtataa aagcacaggg aacctgtgtc tggaatcccg cagattcact
     1921 caggtttccc gcacatggga aaatttttcc aactccagaa ttgttgcaat ctgaaatatt
     1981 agcaataagt tcaactcttt tatttgtttc tgacaaatat caacaatttt ggaggaaatt
     2041 tctgcccatt tttccctacg gcatctgggt tgcttaccga taaagatagg agaaaaaaac
     2101 ataatgaagg gggcaggtga tggtgcggac tgttctccgg ttggttatgg gaagtctact
     2161 tctcccattt gcaagcggct cggccttttc tgcggatacg atcccgcaca ttgatcggct
     2221 agaactgcga accgactcac attccaatgc atgtgattct ccaaactatt cctctttagg
     2281 acgttcctcg ccgttcttct ccccaagcac gccctgtact gtatcgaagg atgaatatgc
     2341 tctcactcgt ttcaacttta accaaaaacg tgaaccgttc aatcttcaga acgctcgaga
     2401 tatgccctct ctggtgaacc aaggtggtag cagtttgtat gaagcatcaa atgatattcc
     2461 tctttttcat gatgccctgt ccgctggagt ccagatgatg tatcgggatc cctttgtaac
     2521 ggacaactcc gcaacgccgt taaccaaccg catgggagct caaatcgttc ttaaggggac
     2581 cacagccagt atgaagtatc aagctcagta tggcttctcc gggcaagaaa ccgggaacac
     2641 ttcattgacg acgcccaatg atcgggtagg aggaaaactg gcatgggaat ggaaccttcc
     2701 attcgtcact ccccaaattg aacttagccg attcactagt aatgtggagg gagatctgtc
     2761 tcgttctcaa accgtcgcta accaacaaaa attttctttg ctcttgtccg ctcccaattg
     2821 gcccagccta tcccttgggt atgccaggca gcagaaagac attttcactc gtcctgaggg
     2881 gcctctctcc aatgccattt ccactgaatc agtttccgcg aacatgtcgt ttctccacgg
     2941 ggcgggcaag ggggattggt cctcttatta taaaacgtct caaagtgaat atggagagaa
     3001 tgggtcagag gaggaatttg gatcaaaatt gggtggcacc ttgaatctgt tagaaccggt
     3061 agatcttgcg ccccagtggg gatttacccg gcgagtcaaa tcccgtggga ctatggtaaa
     3121 cgatcgctac tttgccaggc ttgggtcaaa tttccgagtt tccccaactt cgacgcttaa
     3181 cccgggagtt gaattcgccc gggacctgaa tcgatttgat gcccttcgta ctgacacact
     3241 tgcggctaag cttgggtacg cctatcttgc tgtggatgat tccctgcacg tttcgattct
     3301 tggacaatat attctcaaac aacgcactaa tacccaggcc aatcctcaaa tctacgacat
     3361 ttcactgcta ttcaaaaaag atgtccggga ctatttgcat ctgaaccatc ggcaacaaac
     3421 cctcagcctc aaaatcgcac acaaccagca aatgaacacc tcttcttctc aaacccaatc
     3481 cggccagacc tctgccatgt tgctcgtgag catcattccc tgagtactcg gaatctcccc
     3541 tttcctccaa ccatcttttc ccatgacttt ttagaagcat agggagaacg tatcctacat
     3601 ctaatccaga agagaaagat ggagacctca aaagagcacc tccagtaaag ctactcctag
     3661 tgagacaaga tgagaattct gaggagtacg tgagcaggtc caggtggcct gagcactact
     3721 agcggaactc ccaagactcc ttaaaaaaat ttctccttca agaaggggag ggcaggcaaa
     3781 ggaaaaaatg gggtatgccc agagcgacac gactccatac tcaagttcag aatatcggta
     3841 tggtaggagc agaaagcagt ctctgctgtt aaccatacga attttccaat aggagaggtc
     3901 cgctctaatc agtatgaatc attccctgaa ttaaaacgaa ctcactgcta aagactcttg
     3961 tgtaagagtt ctgccttttc gcctgaaagt ttggagcggt tggtcgtgct aataaatgta
     4021 agacagtaag gaaaactttg ggcgcgatct tatgggttca agggctgaag catttcttgt
     4081 tgcctatact tcgacacagg ttgaatattc attggaggat acgcggtcgg aggaatgatt
     4141 tccatcactg acccactact tcccggggtt atcaacatgg ccttactact ggctgcggag
     4201 attggcatgg aagctccagg tgcaggtgga cgaatctcca tgggaacatt gcttatggca
     4261 aacacctgtc cattggacac gtttttacca ttcgttcgcc ggttagacct gaggacattc
     4321 ttgttcatct gattctcacg gaccaaatct atcccattgt ctgctaaccc gtatttttgg
     4381 agtgaattgg cgtgttcctc ggaagattcc ggtccccagc ctttagcctt tttatccgat
     4441 agggatactt ctggtgacac cttagccatc acatggagtg tggtgatatt tccagccgca
     4501 ttcaatgtaa aggcatagtc ccactgaacc aggatcttcg agagtgccct gtgcaaaggc
     4561 tccttctgta acctaaccga aatgactttc tccaattcta catccggtgc cacataatca
     4621 aggcccaatt ctttatgcaa ctgaccaagg actgtgcgca gagtcacctg attcaactgg
     4681 cccgtcaatc gtccggaatc gacatgaagc ggaatctgca gatcagaggc caacccccaa
     4741 tccggcagtc cgcatagact gatgcccaga agtactccgc cacacaccat gaagaagtgg
     4801 cgtccgctcc ttagttttcg ttgaattaga tatttcatcg ctcacccctg catgcaccgc
     4861 agaccaatca ctttttgaaa actgccccga ctggtgatgt tttaaccacc catcttattt
     4921 ttgaatttcc caattcaccg gggtactgcc aggaaacccc accgaaccga ttttcaaacc
     4981 atccatcagc cacaccgcaa ctttgccatt ggtccgattt tgcaagataa catctccctt
     5041 accatcccca ttcgcatcac tcacctgcct aatttcccat tcgaggggta tgccacctat
     5101 aaaacctgcc aggggtgtcg tcgccccatt catcagccac acccccacca ttccagtttt
     5161 tttattgcgc cagatcacat cccctttact gtccccattg acatctccaa gtagttgaat
     5221 ctcccaatcc agcgaagtgc ttgcaggaaa gcccaccgag gtaatggtca acccattcat
     5281 taaccagacc gccactgtgc cattcgtccg gttctgccaa accaggtctg ccctcccatc
     5341 ggcattgata tcacccactt gctgtatttc ccacactttt ggagtgcctc caaggaggcc
     5401 gactgagtga atcgacaacc catccataaa ccagacagtc accattccac tatcaggatt
     5461 gcgccagatc acatccgctt tgccatcccc attgatatcg ccgaccccct taatcaccca
     5521 ttcggctgaa acaccaccga gaaagccgaa cgattgtatt gtcccctcct tcataagcca
     5581 aatacccacc actccactta tggtgttttg ccaaaggatg tctccttcac catcaccatt
     5641 gatatctccc acctgcttaa tcttccattc cgtggaagta cttccaggaa aaccgaccga
     5701 gataatagtc agcccattca tccgccaaac agcgacttca ccgctactcg catttcgcca
     5761 aagcacatta gtcgtgggag gggtgatatc gggagcgggg ctctctgctg gttgaacgat
     5821 gacggagtac atactgccgc tactcccccc gctggcattg cccccgagag tgatcgtccc
     5881 ggccggaaag gcccgagcat agaggtggag ggt
//
LOCUS       DATIAL010000126         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_130180, whole genome shotgun sequence.
ACCESSION   DATIAL010000126 DATIAL010000000
VERSION     DATIAL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130180"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VLA60_03980"
     CDS             <1..959
                     /locus_tag="VLA60_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF08548.1"
                     /translation="LGAVGCAQKEKSMFTLQDEHYQQILERQKAGINPESQAPVDLTQ
                     VMNSDEFEKLGDAHLQQGDFQTAKIQYEKVLEASPEQIPARYKLAVIYLEKGSPQNAY
                     DQFHQILDYDLNFAPAYEGMGRALLKMEKDQEAEKEFQAALLYDAEMWTSKNYLGIVA
                     DRRHDHKEAIHLYEVALEKRADDPSILNNLGMAYYLDNQYEEAVRTFQHAIQVGGSND
                     KISNNLGLALTKLGHFDLAYEAYARGMDSAKAYNNLGVAFLEVGKFARASRCFEKAIE
                     TQPTFYEKAKENLMLSNRMLAKLPMVQQQALIRRDPVCVGAI"
     gene            complement(1767..4457)
                     /locus_tag="VLA60_03985"
     CDS             complement(1767..4457)
                     /locus_tag="VLA60_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08549.1"
                     /translation="MDRQHFFQELKQMDIPGLEAALRSSQAFVHTLMDSINIGICHVD
                     TQGQILSLNLEGARILHRTETSCLGQSFHAHAECLHIDLVTQEEHCPITRSITTGKSI
                     WRPQLLIRRPSGETRWVEFQSTPLTNPRHSGALIIFRDLSQELRQHEAHQHLASMPEE
                     SPNPIVEVDAQGHLAYANPAMIRLLDTYGFREDGVPMVLPATLTQLALGCLKTAIPAR
                     GLTVIVEHHHFDWTFSPLQEKGLVRGYGLDITHHVRVSQTMTELHSQFSSLVDSAHEG
                     IISADLHGTIVSWNPAAESIFGYQPDEVLGQSIVSLLEEGYRDMYRKGLGDISYGRSS
                     TFPLQQPLELTGLRKSGESFPLEISSTSWKVGMDTHYGFILRDISDRKRLEHALIAEK
                     DHLVTTLQSVAEGIVTTDREGRISFLNPLAEQITEWTTQEALHRPFQEIFRLTGDSQK
                     IDEESSLQFSSRTLVLSEQDAPHQLLTKHGRQRKISLREGPIRDHSGHLVGTVIVFRD
                     ITDQSRHQEEQQRINKLNSLGVLAGGLAHDFNNILTTILGNVFVAKLRMVSNDPLTQN
                     LEQAEQACLRAKELTQQLLTFAKGGAPIKTSIALGDLLRKSTIFALSGSSISCHFDIP
                     KDLWPLDADPGQIRQVFQNITLNARQAMPHGGHLSIKVENVGLKDPSTLPSSSLIPGN
                     YVQVSFEDQGNGIEAHQLPNIFDPYYTTKPGASGLGLATAHSIIQQHHGYISVTSKVG
                     VGTTFTVYIPSTYVTPESETQHISALPRKCRGRILVMDDEHSIRRMVEDALTQFGYDV
                     VSVPDGQTAIDLFSKALADGMKFEVVILDLTIPGAMGGVKAIQHLRNLDPHIKAIVTS
                     GYSDDPIMCHFQDHGFQGILIKPYKIFDLAKTLESMFSRN"
BASE COUNT         1112 a          995 c         1211 g         1181 t
ORIGIN      
        1 atttgggtgc cgttgggtgt gcccaaaaag aaaaatccat gttcactctc caggatgaac
       61 attaccagca gatcctggaa cgacaaaagg cggggatcaa tccggaatct caagcgcctg
      121 tggatcttac tcaggtcatg aacagcgacg agtttgaaaa actgggagat gcccatctac
      181 aacaaggcga tttccagacg gcaaaaatcc agtatgaaaa agtgttagag gcctcgcccg
      241 aacagattcc tgcacggtac aagttagcgg tgatctattt ggaaaaagga tcccctcaga
      301 atgcctatga ccaatttcac caaatcctgg attatgattt gaattttgct ccagcttatg
      361 aggggatggg acgggcattg ctgaaaatgg aaaaggatca ggaggcggag aaggaattcc
      421 aggcggcctt attgtatgac gctgagatgt ggacctctaa aaattacctt ggtattgtgg
      481 cggatcggcg tcatgatcat aaagaggcga tccacctgta tgaagttgcc ttggaaaaga
      541 gggcagatga tccttcgatc ttgaacaatt taggtatggc gtattacctt gataaccaat
      601 atgaagaggc ggtcagaact tttcaacatg cgattcaggt tggtggatcc aacgacaaaa
      661 tttcgaacaa tctcgggtta gctctcacca aacttgggca tttcgatctg gcctacgaag
      721 cctatgcccg aggaatggat tcggccaagg cctataataa cttgggtgtc gcatttttgg
      781 aagttgggaa gtttgcgcga gcttcccgtt gttttgagaa agccattgaa acccaaccga
      841 ctttttatga aaaagccaaa gagaatttaa tgctcagcaa tcgaatgctg gcgaaacttc
      901 ctatggttca gcagcaagca ttaatccgac gggaccccgt gtgtgtcggg gcaatttgac
      961 cgatctcgtt gagggaatcg tcgcggccat gctttctgcc cggaattgat tggccggctt
     1021 gagcgatttt tttgactggg gtgatcggga gtgcagttgt tatgtaagaa atggatttcc
     1081 tctgataatt agttgaaatg atcattacga ggatcctctt cgaatccacc ttcagatgga
     1141 attcgctcct atcaggagga aaatccgctg gtccttccat cctttcaact cttcgtgttg
     1201 aatctctatg aactcaattc tcctgtccta taagcttgtg cgtgtggctt gacgggtaag
     1261 cctcctggtt tcctggcatg tgccctttcc tttttctctt ccgaagtcat taaggacatc
     1321 caacatagaa gtgggtatga aatctaacgg gaatagagtg aggttcaggt cgttgccaga
     1381 cttgagcatg aacgacgacc agtatatggc ctcactggat gtggacaaac tcaaccaaga
     1441 gatatccgga actaaactga ggcaaagata ttggcagacg catcctccaa agagttcatt
     1501 gatggcagga ttgttgatcg gtctaagcta ttccagcgta agttctctgg attaaaggat
     1561 gagaacgata aacaggtgat gtctcgggag ggaataataa ggaattttgc gtcaaagtcg
     1621 aatcgctagt ccttaaattg agataaatgc ctcccttttt attttgaata tgagggttgg
     1681 ggcgtccgtc gttcaggata cgacttgagt cggatgacct actaaatacc atgagggtct
     1741 tgaggaggtc agtgggttgt tttacgctag tttcgagaaa acatggattc aagagttttt
     1801 gcgaggtcga agattttata gggtttgatc aagattccct ggaatccatg gtcttgaaaa
     1861 tggcacataa tgggatcgtc tgaatatccg ctggtgacaa tagccttaat gtgaggatct
     1921 aaatttctca gatgctgaat ggctttaact ccccccatcg cgccggggat ggtaagatca
     1981 aggatgacta cttcgaattt cattccatcc gctagtgcct tggaaaacag gtcgatagcc
     2041 gtttgcccat ccgggacgct gacaacatca tagccgaact gcgtcagcgc atcctcaacc
     2101 atccggcgaa tgctgtgttc gtcatccata accaggattc gtcctctgca ttttctggga
     2161 agggctgaaa tgtgctgtgt ctcactttcc ggggtgacat atgtggaggg gatatacacg
     2221 gtaaaggtcg tgccgactcc gacttttgat gtcacgctga tgtatccatg atgctgctgg
     2281 atgatgctat gggcggtggc taaccccaac ccggatgcac ccggtttggt ggtgtaatac
     2341 ggatcaaaaa tatttggcaa ttggtgggcc tcaattccgt tcccctggtc ttcaaaagac
     2401 acttgcacat agttcccagg gatgagtgag gatgaaggaa gggtcgaggg atcttttagt
     2461 cctacgtttt ctactttgat gcttaagtgg ccaccgtgag gcatggcctg tctggcattg
     2521 agggtaatgt tttgaaaaac ctgccggatt tgaccggggt ccgcatcaag aggccaaagg
     2581 tccttgggga tgtcgaaatg acaactgatt gatgaccctg atagggcaaa aatcgtactt
     2641 tttcgaagca gatctcctaa cgcgatggat gtctttatcg gcgctccgcc cttggcaaag
     2701 gtcaggagtt gttgggtcaa ttctttggcg cgcaagcagg cctgctcggc ttgctcgagg
     2761 ttttgcgtca gagggtcgtt ggacaccatt cgcaatttgg ccacgaagac gttccccaat
     2821 atcgtcgtga ggatattatt gaaatcgtga gccagccctc ccgccaaaac ccccaaagaa
     2881 ttgagcttgt tgattcgttg ttgttcttct tggtgacggg attggtctgt aatatctcgg
     2941 aaaactatta ccgtacctac gagatggccg ctatgatcgc ggatcggtcc ctcccgcagg
     3001 gaaatcttcc gttgccggcc atgtttggtc agtaattgat gaggggcatc ctgctcagag
     3061 aggacaaggg ttctggaaga aaactgcaaa gacgattcct catcaatttt ttggctgtca
     3121 ccggtcaggc ggaaaatttc ttggaatggt cggtgcaagg cttcctgggt tgtccattcg
     3181 gtgatctgct ccgccaaagg gtttaagaaa gaaatgcgtc cttctcgatc agtcgtgacg
     3241 atcccttccg cgaccgattg aagcgtggtg accagatgat ccttttcagc gatgagggca
     3301 tgttcgaggc gtttccggtc tgaaatatct cgaaggataa agccgtaatg ggtgtccatg
     3361 ccgactttcc aacttgtgga agaaatctcc aagggaaatg attcgccgct tttccgtagg
     3421 cccgtcagtt ccaagggctg ctgaagagga aaggtcgaag atcggccata actgatatct
     3481 cccagtcctt ttcggtacat gtcccgatag ccttcttcca ataaggacac aatcgactgt
     3541 cccaggacct cgtcaggctg ataaccgaag attgactcgg ctgcaggatt ccacgagaca
     3601 atggttccgt gtaaatcggc agaaatgatc ccttcatggg cggagtccac cagtgaggaa
     3661 aattgagaat ggagttcggt cattgtctgg ctgacccgaa catgatgcgt gatatccagg
     3721 ccatatcccc gcacgagacc tttttcctgg agaggcgaaa aggtccaatc aaagtggtgg
     3781 tgttcaacaa tgaccgtgag acctctggcg gggattgcag ttttgaggca tcccagggcg
     3841 agctgtgtca gtgtggctgg aagcaccatt ggcacaccgt cttcacgaaa tccataggtg
     3901 tcgaggaggc ggatcatggc agggttggca taggccaaat gtccctgggc gtccacttcc
     3961 acgatgggat tcgggctttc ttctggcatc gacgctaggt gttgatgcgc ttcgtgttgc
     4021 cggagttcct ggctgagatc acgaaaaata atcagcgcac ctgagtggcg cggatttgtc
     4081 agtggtgtgg attggaattc cacccagcgg gtttccccgc ttggccgtcg gatcaaaagt
     4141 tgaggcctcc atatggattt tcccgtggtg atcgatctcg tgatcgggca atgttcctct
     4201 tgtgtgacaa gatcaatatg gagacattcc gcgtgtgcat gaaaggattg cccgaggcat
     4261 gaggtttcgg ttcgatgaag aatcctggcg ccttccaggt tgagggaaag aatctggcct
     4321 tgggtatcca cgtggcaaat gccaatgtta atggagtcca tcagggtatg cacaaaggct
     4381 tgggaggaac gtaatgccgc ctctaatcca gggatgtcca tttgtttgag ttcttgaaaa
     4441 aaatgttgcc gatccactca gcgattcctt cctatcatag cgacgacatc gtgtgaacg
//
LOCUS       DATIAL010000127         6610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_345634, whole genome shotgun sequence.
ACCESSION   DATIAL010000127 DATIAL010000000
VERSION     DATIAL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345634"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VLA60_03990"
     CDS             complement(<1..1700)
                     /locus_tag="VLA60_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08550.1"
                     /translation="MTSDTPRQDTPPSATPPRSRKALKWFLWLGLGMLALLLCAGLLL
                     TYWFPSDMVRQELEIRLSELVQGTVRIQSLSFNLLTGLQVSQLELTKPGQTPLTLEHL
                     TLDYSLFGLLKGTFTINEISIDQADISLNLPELTKAAPEPPPPPSSEPAALPTLPVSI
                     DLETLKIIESNIQLVVSPDLTVNLTHLNLQSSGAVSSDDAHLNGTLQVKQVAVALQGK
                     HIQLPLVVKFDTAIHLSTQHLDLKQLTIESDPALRLTLAGTINEFLTQKAVNLSLADT
                     NLDLGIIMALAKDFVPPEFATASIRGNLSPSFTLTGSLPDSGFEGTIHAGLDGKNVEL
                     HLPGHALTVGPTDLAFKAKELQIKENLPLMGNISAKLSIHDVTFQAYGIQNFGLSLQS
                     DYQASGPFSGILQVSGTTVLPGDLLGTPFNLPFAVTLDTSGNHHTRHIEVKHLNADLS
                     PYATLQMNGVIQPHPAPREGMDASLKLRLSPKINALLSLIPKDRLQGLVLQKGSEPDT
                     FVLHATGSLHPDFHPEWAKATAALKLSSLQAQVKPFGAEGTMHQLTFLLSSGYQESNG
                     A"
     gene            complement(2042..2294)
                     /locus_tag="VLA60_03995"
                     /pseudo
     CDS             complement(2042..2294)
                     /locus_tag="VLA60_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
     gene            complement(2399..3025)
                     /locus_tag="VLA60_04000"
     CDS             complement(2399..3025)
                     /locus_tag="VLA60_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HSF08551.1"
                     /translation="MLLSLNTFGCQSIYYDAMEKIGYHKRDLLVSDVKKARDAQQEAK
                     EQFKSALDRFTKTLNIEGGELQDKYDVLNAEYEQSEAKAQAVRDRIASVEDVSEALFD
                     EWEAELKEYSSTALRKNSQKQLTQTRTQYAQLIKAMKRAETKMDPVLAKFKDQVLFLK
                     HNLNAQAIASLKSELVSVEGNIASLIKEMEASIKEADSFIASMEKEKT"
     gene            complement(3133..3570)
                     /locus_tag="VLA60_04005"
     CDS             complement(3133..3570)
                     /locus_tag="VLA60_04005"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HSF08552.1"
                     /translation="MELIQQLVNNLGVNEGQAKGGVGLLFKLAKEKLSSGEFQQLAEK
                     VPGVRDLLGAAPAPSPAASAGGGMMGALGGIAASLGAGGLGNKIGGLENLANLASGFS
                     QLGLSPDMIGKFLPIVLSFVQNQGGDSMKGLLEKVLKPAASAT"
     gene            complement(3759..6224)
                     /locus_tag="VLA60_04010"
     CDS             complement(3759..6224)
                     /locus_tag="VLA60_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08553.1"
                     /translation="MSDFEKLGVFYLGRPYDLATKSAKSGLLLYDSKDLVTHAVCVGM
                     TGSGKTGLCIGLLEEAAIDNIPAIIIDPKGDIANLLLTFPDLHGRDFLPWINHDDAKK
                     KALTPEAFADKEAAKWKAGLEAWGQDGARIAKLREAADFLVYTPGSTAGLPVSILQSF
                     AVPSPNILEDAELLGDRVGTTATSLLNLVGLDADPLQSPEHILLANVLKTSWEAGQDL
                     TLSSLIQQIQTPPFSKLGVMDLESVFSSKDRFALAMRFNNLLGAPGFSAWLEGQPMDI
                     DQILHSPTGKPRIAIFSIAHLNDIERMFFVSLLLTQMVSWMRSQSGTTSLRALLYMDE
                     VFGFFPPIKNPPSKAPMLTMLKQARAFGLGVVLATQNPVDLDYKGLGNTGTWFIGRLQ
                     TERDMARVLDGLEGAAANSQGNFNRQRMEQIISGLGNRVFLMNNVHDDGPEVFETRWA
                     LSYLRGPLTRTQIKTLMDPVKAALSDSAASPASTKTLTAASQSHTGTLPKGSANTHPP
                     VLPPDIRQYFTPIRGARPANVSLMYYPRLYGATSVHYQDPKTKLDEVREVQALVEITT
                     GAVPVHWDQADPLDIPPADLNTHPADGAQFGELPGAATQAKSYKEWEKDFEDWVYRTQ
                     TLDIYRNASLGVTSGPHESERDFRLRLQQLVRERRDEETDALRKKYAGKMTTLEDRIR
                     RAEQAVQRESEQAKQQKLQTVISIGATLLSVFMGRKTVSRSSMGQATSAVRGMSRTMK
                     EGKDVDRAEDNVDALQQQLAALEAELQEEIQSLTAQWDVETIPLETLNVRPKKSNIST
                     RLVSLAWAPYWTETNGQQTPAWL"
BASE COUNT         1565 a         1554 c         1840 g         1651 t
ORIGIN      
        1 gcaccattgc tttcctgata gccggaagaa agcaggaagg tgagttggtg catggtgcct
       61 tctgcgccga agggtttcac ttgagcttga agggaggaca gcttgagagc cgccgttgct
      121 tttgcccatt cgggatggaa atcaggatga agtgatccgg tggcgtgcag cacaaaggta
      181 tccggttcgg accctttctg gagaacgagt ccttgaagcc ggtctttggg aatgagggag
      241 agtagggcgt taatctttgg tgacagacga agcttcagcg aggcatccat gccttccctc
      301 ggggcaggat gaggttgtat cacgccgttc atctgtaagg tcgcataagg gctaaggtcg
      361 gcgttcaaat gcttcacctc tatgtgacgg gtgtgatgat tgccactggt atcgagggtc
      421 acggcaaaag gaagattgaa tggtgtgcca agtaaatcgc cgggtaatac cgtggtgccg
      481 gagacctgca ggattccgga aaagggtccg gaggcctgat agtcgctctg gagactcaaa
      541 ccaaagtttt gtattccata ggcttgaaac gtcacatcat gaatagaaag tttcgctgaa
      601 atgtttccca tcaacgggag gttctccttg atctgaagtt ccttggcttt gaaagccaga
      661 tctgtgggtc ccacggtcag ggcgtggcct ggcagatgca attccacatt ttttccgtct
      721 aatcccgcgt ggatggtccc ctcaaatccg gagtcgggaa gtgagccggt gagggtgaat
      781 gagggagaca gatttccgcg aattgaagcc gtggcaaatt ccggcggtac aaaatctttg
      841 gcgagggcca taattattcc gaggtcaaga ttcgtatcgg caagagaaag attcacagct
      901 ttttgggtta agaattcgtt gatggttccg gcaagtgtga gacgaagggc cggatccgat
      961 tcaatggtga gttgcttcag atcaagatgt tgagtcgaaa gatgaatggc ggtgtcaaac
     1021 ttcacgacca ggggcagttg aatgtgtttt ccttgaagcg ccaccgcgac ttgcttaact
     1081 tgcagcgtgc cgttgagatg tgcatcatcc gatgaaacgg ctcccgaact ctggagattg
     1141 agatgggtca ggttcaccgt gaggtcgggc gataccacaa gctggatatt gctctcgata
     1201 attttaaggg tttccagatc aatcgagacg ggaagagtgg gaagcgctgc cggttcagaa
     1261 gagggtgggg gaggtggttc cggtgcggcc ttcgtgagtt caggcagatt gagtgatatg
     1321 tctgcctgat cgatggaaat ttcgttgatg gtgaaggtgc ccttcagcag tccgaaaagg
     1381 ctgtaatcca atgtcaggtg ttcgagtgtg agtggggttt ggcccggctt ggtcaattcc
     1441 aattgactga cttgaaggcc ggtcagcaga ttaaacgaaa gagattggat tctgaccgtt
     1501 ccttgaacaa gctcggagag ccgaatttcc aactcttgtc gaaccatgtc cgaaggaaac
     1561 caataggtca gtaacagacc cgcacagaga agaagggcca acatgcccag gcccaaccag
     1621 aggaaccatt ttaaggcttt cctggatcgt ggtggcgttg ccgaaggagg cgtgtcttgt
     1681 cggggggtat cggacgtcat ggaagagaaa gaatcctatt accggaaagc gaccagatct
     1741 cttttgttat tggagttgag actccaatgc tatgtaatcg ctattaccta agagtgtagt
     1801 caactttcct cattaaataa aggaaatgcc tctttgcgag gatcataaat gagggggcgt
     1861 gaggagagta agagatcggc aaatctttcg gggtgcctcg gcacatattt cgtcatcccc
     1921 gcaaggattt agcgcagatc aatctgaaat ggagaccaaa gggatttccg aaacaggttg
     1981 gtgcgaatga ctgaggtggg gaaatcgata agaggataaa tgcgggcgac ggtgccgatt
     2041 attaagagcc tttgataatt cggtaggcaa aaaggagcac gaaggctccg ccaatggcga
     2101 tgccaagact atgcagatta aatccggtga catcaccata gtcaaaatat gtgctgatca
     2161 tccccgccca ccatggcgcc ggaaatcccg atcaagatgg tgataatcat tccgccaggg
     2221 tcctttcccg gcataatgac ctttgccagg atccctgcaa tgaggccgaa cacgatccag
     2281 gatatgatgt ccaatgagga atctccccgt gagccagtat gaacgacaat atgtgtcact
     2341 cgcctgccta ttgccggcag gtcgagtgac acgttgagac gggcaggggc ccaaattgtt
     2401 atgtcttttc tttctccatt gaggcaataa aggaatcggc ttctttaata gacgcttcca
     2461 tttctttgat gagagaggca atgttgcctt ccaccgagac caactcactt ttcagcgacg
     2521 cgatggcttg ggcatttaga ttgtgtttca gaaagaggac ttggtctttg aatttggcca
     2581 agaccggatc catttttgtt tccgcccgtt tcatggcttt gatcaattga gcatattggg
     2641 tgcgtgtttg tgtgagttgc ttttggctgt ttttgcgcaa ggcggtgctg gaatattctt
     2701 tcagttcagc ctcccattca tcgaataagg cctcagagac atcttccacc gacgcgatgc
     2761 gatcccgaac cgcttgggct ttggcctcgc tctgttcgta ttccgcattc aggacgtcgt
     2821 atttatcctg taactcaccc ccctcaatgt tgagggtttt ggtaaaccgg tcaagagcgg
     2881 atttgaattg ttctttggct tcctgttggg cgtctcgagc ttttttcaca tcactgacca
     2941 acaggtcgcg tttgtggtag ccgatttttt ccatcgcatc ataatagata ctctgacaac
     3001 cgaaagtgtt cagactcaag agcatgatta accatgtcag gtaaggaaag agtctgcaag
     3061 aacctcctga agaatagtga aagtacatca tggcatcctt gtgcaaaggg ttcatattca
     3121 ttggaacaat catcaggtag cggaagccgc aggctttaag actttttcca gtagaccctt
     3181 catggaatcc ccaccttgat tttggacgaa ggaaaggacg atcgggagaa attttccaat
     3241 catgtcgggg cttaatccca gttgggaaaa tccacttgcc aaattggcaa ggttttccaa
     3301 tccccctatc ttgttcccta atcccccggc tccgagtgaa gcggcgatcc ctcctaacgc
     3361 ccccatcatg ccgcctcccg cagaggctgc tggggaggga gctggtgctg cccctaagag
     3421 gtccctgacc cctggaacct tctcggcaag ttgttggaac tcgcctgagc tcaatttttc
     3481 tttcgcgagt ttgaacaaga gaccgactcc gcctttggct tggccttcat tgacacccag
     3541 attgttgacg agttgttgaa ttaattccat ggcaagacct cctgatgaac aatcgaatgc
     3601 atggacacac gggtaaaaga tcagtgaggg gtgggccgat cgctcccact tcagtcgatc
     3661 attgatgatg tagcatcagc cgtaggtgat aagatacggg atatgccgct ctaaaagaaa
     3721 ccagaatggc caaatctttg gctggaaact ctggagggtc aaagccacgc tggagtctgt
     3781 tggccattgg tttctgtcca atacggggcc cacgccaacg acaccaacct tgtcgaaata
     3841 ttcgactttt tggggcgcac attgagagtc tccaggggaa tggtttcgac atcccactga
     3901 gcggttagtg attgaatctc ttcttgtaac tccgcctcta acgcagccaa ttgttgttga
     3961 agggcgtcca cgttatcctc ggcacggtcc acatccttgc cttctttcat ggttcggctc
     4021 atgcctcgaa cggcggatgt ggcctggccc atcgaagacc ggctgacggt tttacggccc
     4081 atgaataccg ataagagagt cgcgccgatg gaaataaccg tttggagttt ttgttgtttg
     4141 gcttgctcgg attcccgttg gacggcctgt tcggcccgcc ggatgcgatc ttcgagtgtc
     4201 gtcatttttc cggcatattt tttacggagt gcgtcggtct cctcatcccg acgttcgcgg
     4261 acgagttgtt gtaatcgaag gcggaaatca cgttccgatt catgtgggcc ggaagtcacc
     4321 cctaacgagg cattcctgta tatgtccaag gtttgcgtcc ggtagaccca atcctcaaaa
     4381 tccttttccc attccttata ggatttcgcc tgggtggcgg cgcctggcag ctccccaaac
     4441 tgtgctccgt cagcaggatg ggtattcaaa tcggcaggtg gaatatcgag agggtctgct
     4501 tgatcccaat ggaccggtac cgcgcccgtg gttatctcta ccagggcttg gacctcacgg
     4561 acttcatcca attttgtttt gggatcctgg taatggaccg atgtggcacc atacagtcgt
     4621 gggtaataca tgagggagac gtttgcgggc ctggctcccc gaatgggtgt gaaatattgg
     4681 cggatgtccg gaggaagaac cggtggatgg gtgttggcgc tccctttagg aagtgttccg
     4741 gtatgagatt gagaagcggc ggttagggtt ttggtggaag cgggtgaggc ggcagaatcg
     4801 gacagcgcag cttttaccgg atccatcaag gttttgattt gcgtccgggt taaaggtcct
     4861 cgaagatagg acaatgccca acgggtctca aaaacttccg gcccatcgtc atggacgttg
     4921 ttcatgagaa acacccggtt cccgaggccg gagataatct gctccatcct ctgacgattg
     4981 aagtttcctt gactgttggc cgcggcgcct tccaatccat ccaatacgcg ggccatgtcc
     5041 cgttcggttt ggagtcgtcc gataaaccac gttcccgtat tgcccaatcc tttgtaatcc
     5101 aggtcaaccg ggttttgagt ggcgagcacg acgccaaggc caaaggctcg agcttgtttc
     5161 agcatggtga gcatcggggc tttggatggc gggtttttga taggaggaaa gaagccaaag
     5221 acttcatcca tatagagcag tgcgcgcaga ctggtggtgc ccgattggct tcgcatccaa
     5281 ctgaccattt gagtgagcag gagactgaca aaaaacatgc gctcgatgtc attcaggtgc
     5341 gcgatggaaa aaatggcgat gcggggcttg ccggttgggg aatgcaaaat ctgatcgata
     5401 tccatcggct ggccctccag ccacgcggag aaccctggcg ctcccaataa attattaaaa
     5461 cgcatggcta atgcgaagcg atccttcgag gaaaaaaccg attccaaatc catcacgccc
     5521 aactttgaaa acggcggggt ttgaatttgt tggatgagcg aggaaagcgt gaggtcctgt
     5581 ccggcttccc aggaggtttt caggacattg gccagcagga tatgttcggg gctttggagg
     5641 gggtcggcat ccagaccaac cagattgagt aagctggtcg ccgtggtgcc cacccggtcc
     5701 cctaaaagtt ctgcgtcttc aagaatattg ggggagggca cggcaaacga ttgaagaatg
     5761 gatacaggaa gtcctgccgt gctgccaggg gtataaacca gaaagtccgc tgcttcccgg
     5821 agtttcgcga ttctggctcc atcctgcccc caggcttcca agcccgcttt ccatttggcc
     5881 gcttccttgt ccgcgaaggc ttccggcgtg agggcttttt tctttgcgtc atcgtgattg
     5941 atccagggaa gaaaatcgcg gccatggaga tcgggaaagg tcagtaagag gttggcgata
     6001 tcgcctttgg ggtcaatgat aatggccggg atattgtcga tcgcggcttc ttccaatagg
     6061 ccaatacaca agccggtctt gccgctgccc gtcatgccga cacagaccgc atgggttacc
     6121 agatctttcg agtcatagag gaggagaccg gatttggctg atttggtggc gaggtcgtat
     6181 ggcctcccta aataaaacac cccgagcttt tcaaaatcag acatgatcag ccactccctt
     6241 cgttcacttg caaattgaag ttccctggtt aaaaatggat gagggaaact cgtttcctgg
     6301 ttattctttt caaatgtact gtagccctga gcgcggattg ccctgagcca gggatacgat
     6361 accaaagaag ccaggaccgt cgcatcacat aaggattcag gagaccaata ccagttcagc
     6421 caatttagag ctcaacctta tgagccctat ggcaaattga gatccttagt cagttgacca
     6481 tcaatcacat ttgttaggct cgaatatttt ttaagcagcc aggtggatat tcaatatcct
     6541 gaaggaagcg gctcaatacg gaatgaggag agtctcatga ccataacgaa taagcagtca
     6601 gggacgaacg
//
LOCUS       DATIAL010000128         2721 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1205347, whole genome shotgun sequence.
ACCESSION   DATIAL010000128 DATIAL010000000
VERSION     DATIAL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205347"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VLA60_04015"
     CDS             complement(<1..756)
                     /locus_tag="VLA60_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08554.1"
                     /translation="MFKVKKRPLKKEKPPVLYNILENYTDYDPPGNVMVCAMTEPEDL
                     EMHARVLSESYDIPLEAITGLLQDGGIYLYPHEGTLVTKGAFVCRVDPLGKDPKHTWV
                     MDLEQYAAAERMRQSYGVTLEEGMERVFYRGLPQELQDRLRQKNLGIDLTKVDSGNPC
                     SGDIQFIDFRKDWSPHFKRKCVMPDGRLIETSGLHDFAELHGISVDETRALFDHGGTL
                     ALKDGGGLACQIINGQPSVARFNSRQFSKAKALA"
     gene            974..1549
                     /gene="pyrE"
                     /locus_tag="VLA60_04020"
     CDS             974..1549
                     /gene="pyrE"
                     /locus_tag="VLA60_04020"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HSF08555.1"
                     /translation="MPKNTLIQAFHQLEAFKWNQQEGFRLASGKNSPYYVDCRIVLAH
                     PHSRHLVAQLAYHLLKSLDFTLIGGLEIGAIPLATCISDYGYRADPQREWRTFVVRKQ
                     PKDHGLGKLIEGTIHSGETALVVDDVLTTGGSLIKAAEAARAQGLIVTHGLVVVDRSE
                     EEGKSNLAQMGIQLLPLLTLEDLRQAPTSPR"
     gene            1714..2532
                     /locus_tag="VLA60_04025"
     CDS             1714..2532
                     /locus_tag="VLA60_04025"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HSF08556.1"
                     /translation="MDSPFKDGIECAKLDDGPTSFQHKGGVTMKRTIIGIDRAKNVFE
                     VYVEDEGGQGITRCRLSRKKVLPWFANRAPALVGMEACGGAHHWAREVTKLGHEVRRI
                     APQYVKPWVQTNKSDAADARAICRAVQEPGMRFVGLASEAQQAVQALHRVRSRYLNNP
                     TALGNQTRGILLEYGVAIPTGVASVRNALALLLTEDRWSGLVRQFLADQQAELEEWDS
                     KIEAVTAQIKREQARQPICQRLEQARGIGPLGASALWAKMAGQAYRNGRHVAAA"
     gene            2536..>2721
                     /locus_tag="VLA60_04030"
     CDS             2536..>2721
                     /locus_tag="VLA60_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSF08557.1"
                     /translation="MVPKHTGTRGKIRLGRISKRGDRYLRQVLIHGARAGVRQVGEKP
                     DCLICWIRRVVARRGMNK"
BASE COUNT          643 a          724 c          721 g          633 t
ORIGIN      
        1 ggcgagagct ttagctttac tgaattgtcg tgaattaaag cgggccacgg atggctgacc
       61 attgatgatc tgacacgcaa ggcctccacc atctttcagt gcaagagtgc ccccgtgatc
      121 aaacaaggca cgagtctcat ccacgctgat cccatgcagt tccgcaaaat catggagccc
      181 actggtctca atcaatcgtc catctggcat gacacatttt cgcttaaaat ggggagacca
      241 atcttttcgg aaatcaataa attgaatgtc tccactacat gggttccctg agtctacttt
      301 tgtcaaatca atccctaagt ttttttgccg caaccgatcc tgtaattcct gaggaaggcc
      361 tcgataaaaa acccgctcca tgccttcttc caatgttaca ccataacttt gacgcatgcg
      421 ctcggctgcc gcatattgct caagatccat aacccacgtg tgtttcggat cttttccaag
      481 aggatcaaca cgacagacga atgcgccttt tgtgacaagg gtaccttcat gcggatagag
      541 atatatcccc ccgtcttgaa gcaggcctgt tatggcttca agaggaatat cgtaactttc
      601 ggacaggact cgtgcatgca tctccaaatc ttccggttcc gtcattgcgc acaccattac
      661 atttccaggg ggatcataat ccgtatagtt ctctagaata ttatagagaa ccgggggctt
      721 ctcttttttc aatggacgtt tttttacttt aaacattcaa tacgctcctt cacgctgagt
      781 ttcctttagg ccttggactt ctccaccccc gagggagagg ctaaatttcc acgacaccgg
      841 tgcataccga ttccacacaa acattctcat ttttaacgcc ataatttttt ccgcgcactg
      901 ttgcagatcc cgaagtccta atggtagaag gaaatcgctt atgactcaac cgtaacactc
      961 tcctaaactt cctatgccaa aaaacactct tattcaggct ttccatcagc tggaagcctt
     1021 caaatggaat cagcaagaag gctttcggct cgcctcagga aaaaacagcc cctattacgt
     1081 tgactgtcgt attgtattgg ctcatccgca ctcacgtcac cttgtggctc aattagccta
     1141 tcacctgctt aaatccttag atttcacact cattggcgga ctggaaatcg gagcaattcc
     1201 cctggccacc tgtatttcag attatggcta tagggccgat ccacagcggg agtggcgaac
     1261 gttcgttgtg cgcaaacaac ctaaggatca tggattaggc aaattaattg aaggcactat
     1321 tcattccggg gaaacagctt tagtggtcga cgatgtttta acaacaggcg gatcattgat
     1381 aaaggccgcc gaagcagccc gcgcccaggg cttaattgtg acgcatgggt tagtggttgt
     1441 ggatcgatct gaagaggagg ggaaaagcaa tctggctcaa atgggcattc aattgcttcc
     1501 tctactcacc ctggaagatt taagacaagc gcctacctcc ccccggtaag tcctcggaac
     1561 atttcccacc acggttcaaa accttagccg gttcttgtgg cctcaacggg taggcatcct
     1621 atttgtcaaa aattatccag tccaaattga tcgatgatga ccattttaac ttcataaccc
     1681 cggcggtact ccccccctat tcaggataat ggtatggact ccccctttaa ggacggcatc
     1741 gagtgtgcca agctggacga cggaccaacc agctttcaac ataaaggagg agtcaccatg
     1801 aagcgtacca tcattgggat cgatcgtgcg aagaatgtgt ttgaagtgta tgtggaagat
     1861 gaagggggac agggcatcac acgctgccgg ctgtctcgaa aaaaggtgtt gccgtggttt
     1921 gcgaatcgtg cgcccgccct cgtcgggatg gaagcctgtg ggggggcaca tcactgggcg
     1981 cgggaggtca cgaagttggg gcacgaggtg cggcggatcg ctccgcagta cgtcaagccg
     2041 tgggtgcaga cgaacaagag tgatgcggcc gatgcccgag cgatctgtcg cgccgtgcaa
     2101 gagccgggca tgcggtttgt cggcctggct agcgaagcgc agcaggccgt gcaagccctc
     2161 catcgggtcc ggagtcgcta cctgaacaat cccaccgccc tggggaatca gacccgtggc
     2221 atcttactgg aatacggcgt ggccattccc accggggtcg cttcggttcg gaacgcgctg
     2281 gctctcctcc tgaccgagga tcgatggtcc ggcttggttc ggcagttctt ggccgaccag
     2341 caggccgagc tcgaggagtg ggatagcaag attgaagcgg taacggccca gatcaaacgg
     2401 gagcaggcgc ggcaaccgat ctgtcagcgt ctcgagcagg cgcgggggat tgggccctta
     2461 ggcgcctcgg ccctctgggc caaaatggcg ggccaggcct atcggaatgg ccgccatgtc
     2521 gcggcggcgt aagggttggt ccccaagcac accggcacca ggggaaagat ccggctcggc
     2581 aggatcagta aacgggggga tcggtatctc cggcaggtgc tgattcatgg ggcccgggcg
     2641 ggggtgcgcc aagtggggga gaaacccgat tgcctcattt gttggatccg acgcgtggtg
     2701 gcccgacggg ggatgaataa g
//
LOCUS       DATIAL010000129        11101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_22733, whole genome shotgun sequence.
ACCESSION   DATIAL010000129 DATIAL010000000
VERSION     DATIAL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22733"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VLA60_04035"
     CDS             complement(<1..238)
                     /locus_tag="VLA60_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08558.1"
                     /translation="MMKVLLAVEQTRISEGAIQVLSKLTLPRGSDLFLLHVNPVPQKI
                     TGLAKERILKISQQVQEVQKQALEQAREFLGKVEK"
     gene            608..1243
                     /locus_tag="VLA60_04040"
     CDS             608..1243
                     /locus_tag="VLA60_04040"
                     /inference="COORDINATES: protein motif:HMM:NF008320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HSF08559.1"
                     /translation="MLDYTEYLKFFVALVAVINPLGAISMFIALTPGKEMKARQNIAR
                     IASFSATLILLVTLVAGEGVLAIFGISVSSFRVGGGILILLLAISMLQAQRSLTVQTK
                     EEAEESDRKQDIAIVPLSTPLLAGPGSLSTVVLQAHKGTGWAHEAFIALAIVAVGMCI
                     WASLHLAPWISRHLGQTGINIFTRIMGLILAAIAVEFIANGLKGLFPALAG"
     gene            complement(2034..2348)
                     /locus_tag="VLA60_04045"
     CDS             complement(2034..2348)
                     /locus_tag="VLA60_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08560.1"
                     /translation="MYDTQEQVLWFHFDLISRTNRWGLQVLVGLKIKDVINSRLRVTF
                     NNRKKTTLDGKNGIEKPSSLNNTKGTWPESPKGFNPKVQPGKIHLPQWEKNAVWVGKD
                     GR"
     gene            complement(2517..2825)
                     /locus_tag="VLA60_04050"
     CDS             complement(2517..2825)
                     /locus_tag="VLA60_04050"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSF08561.1"
                     /translation="MAKKPLVQPSKNDTRQKSSSSGKLKGMLMKKPLKKPAPLEVEEG
                     LIAAVTESLNFQPANGDGPARIARRAYELFQGRGGHPGQDLDDWLAAEQEILAKDLSS
                     "
     gene            complement(2825..3814)
                     /locus_tag="VLA60_04055"
     CDS             complement(2825..3814)
                     /locus_tag="VLA60_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF08562.1"
                     /translation="MKNMLRAGAHPLWSIILAGGEGERTRPFIEQWLGGHKPKQYCTF
                     VGNRSMLQHTLDRADRLVRPEQKVTVIGRNHRKFLNEAMEGSTSGHVIVQPRNCGTAP
                     GVFLPLTFVRAWDADATVVIFPSDHFVFPEARFLETIRRGIRASHFLQDRLILFGVPP
                     SHPELDYGWINLGEVLGWSHGSCIRHVGSFLEKPDLLEGKQVMASGALWNTLVIVAKV
                     ATLWKLGWRTLPDMMERFEQLGATIGTVHENETLHEIYRDMPILDFSRTLLQRIPEHL
                     GVMEFEEVLWSDWGQPERIAHTLKTLGKKPAFPSEILQRSLAPTDAIPHVEVT"
     gene            complement(4305..4661)
                     /locus_tag="VLA60_04060"
     CDS             complement(4305..4661)
                     /locus_tag="VLA60_04060"
                     /inference="COORDINATES: protein motif:HMM:NF023390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HSF08563.1"
                     /translation="MYRILDESEDGSVGIRIEGKLSAEEYDLLNAYLEHLMQEVGPIN
                     FLCDMATVEGENGQALWEEMTHHLRNVQDYQRIAVVGSRRWLEGGPKVSVPWPRAQFK
                     YFTSDQTDEAWHWVKG"
     gene            complement(5256..5582)
                     /locus_tag="VLA60_04065"
     CDS             complement(5256..5582)
                     /locus_tag="VLA60_04065"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSF08564.1"
                     /translation="MSRQRVLATSERKEPEEPSGATRQSRRMTDKQPRAKTSSRGKNR
                     KPLRPEQEHDGETSFANEGIYPRIAERAYELYQRRGGHHGQDLDDWFLAEQEVMAEET
                     RENIEP"
     gene            complement(5579..6556)
                     /locus_tag="VLA60_04070"
     CDS             complement(5579..6556)
                     /locus_tag="VLA60_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF08565.1"
                     /translation="MSQSHALWSIILAGGEGERTRPFIERWLGYPKPKQYCTFVGNRS
                     MLQHTLDRADRLGAPHQKITVVAQSHQGFAGEALGGQRAGQVILEPNYCGTAPEMFLP
                     LTYVRAWDPSATVVIFPSDHFVFPENRFLEMVRRAIGGSRILQDRLLVLGVRPTHLEL
                     DYGWMNVGGVLGWSGGSCVRQVDSFIEQLDHVQALNAMAKGALWNTSVIVGKVSTIWK
                     LGWQYLPVIMERFERLGKAIGSTHEGRMLRQLYQKMPILDVSSELLQRVPEHLGMIEL
                     DDVLWSDWEGSDRIRDTLNVLGKEPTFPSELVTMPCTSPHALSSLEVTR"
     gene            complement(6884..7129)
                     /locus_tag="VLA60_04075"
     CDS             complement(6884..7129)
                     /locus_tag="VLA60_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08566.1"
                     /translation="MKDILFVSKKQGEMQNLPVSVNENPDLRNDGSSNSLAIIIDNRA
                     QEISQQGGEPRGPSLASWLKAAREILSEDSERPKLTA"
     gene            complement(7406..7576)
                     /locus_tag="VLA60_04080"
     CDS             complement(7406..7576)
                     /locus_tag="VLA60_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08567.1"
                     /translation="MNDHKAGRVNGTIKTGRKEVCAHQRLVDEQVNIQGEKTGHLVCR
                     ECGEVILNPVEA"
     gene            complement(7651..8694)
                     /locus_tag="VLA60_04085"
     CDS             complement(7651..8694)
                     /locus_tag="VLA60_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08568.1"
                     /translation="MNHLFSSSPLAEDLMNTAAVPLRPGRFEKGLALQFLSGQYSGWP
                     VVDSSREILGVVTEFRLLQACSRVNSLDELRVEDTMTTPVYVCEQDPLDVVMKVMVHS
                     QVLRMPVVRDRQLVGVISRGDVLRHSLLRSSPTSQFVFSCAWCERVHDPSEGRVGTDA
                     WQVLDAYLSRHQLTFSDIEPAQTYCPSCLEILQGLQTTSPDVSRAKTEAQTQEVRPCL
                     LVVDDDPAVAGLLDQALQEWGYEVLVARNGREGLDLLGDRCVNGILLDMHMPIMDGRT
                     MLDELRWLGHQMPVLMMSGATEEPLLRRMLQEGAQGFFLKPFHLASVQQACRKILQND
                     GVKAVAASRFHVT"
     gene            complement(8872..9558)
                     /locus_tag="VLA60_04090"
     CDS             complement(8872..9558)
                     /locus_tag="VLA60_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF08569.1"
                     /translation="MPISHPYPSSCAGKSSRNLPRIVLADDHPMVLEGVAKLVEEFAE
                     VVGKVEDGRALLDIVPHLNPDVVVMDISMPSLNGLESARCLTKLIPHCKIIFLSMHGD
                     SSYITAAFEAGASGYLLKRSAGSELKQAVQSVLAGHKYVTPLVLRGDIFPRDSPRMGI
                     PSLNQLTPRQREVLQLIAEGRSTKAIATLLNISPKTVEFHKAKFMETLGMHTIPELTQ
                     FAVACGLVEK"
     gene            complement(9694..10341)
                     /locus_tag="VLA60_04095"
     CDS             complement(9694..10341)
                     /locus_tag="VLA60_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF08570.1"
                     /translation="MVTRPRVLLADDHVLVLEGLAKLVTEDCDLVGKVEDGRVLLQVA
                     PNLQPDVIVLDISMPKLNGLDAARQLKKLLPSVKLIFLTMHGDPLYAKEAFQIGASGF
                     LLKRSAASELMQAINAVMKGQYYVTPAIAKDFLGTFTQEMSAPQAEGNCLTPRQREVL
                     QLIAEGHSTKEMARMLHVSPKTIEFHRAKIIRELNCQNTAELTRYAITHGLVSPE"
     gene            complement(10655..>11101)
                     /locus_tag="VLA60_04100"
     CDS             complement(10655..>11101)
                     /locus_tag="VLA60_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HSF08571.1"
                     /translation="DDIRHLAYQYHPSILDDLGLASALRSLCEDFEKWEGISVTCELP
                     DGARIISQAVGPCLYRVVQESLRNVSKHAQASAVHLILREDEQEITLSIQDNGKGFEV
                     DGLLSRGLGLVSMRERVRLVGGTLLVESQPGYGTTVRVSIPVDAQS"
BASE COUNT         2818 a         3016 c         2592 g         2675 t
ORIGIN      
        1 ttttttcgac cttacccaga aactcacgcg cctgttcaag cgcctgcttt tgaacctctt
       61 gtacctgctg ggagattttc aaaatccgtt ccttcgccag acccgtaatc ttttgaggga
      121 cggggttcac atggagaaga aacagatcgg atccacgggg cagtgtcagc ttgctcagaa
      181 cctgaatggc cccttcagaa atacgagttt gctctaccgc caacaacact ttcatcattg
      241 cgctcctttt caactcataa ccacagaaac cgccttcccc attccagttt accttatgtc
      301 ttcctttcct gtattttctt ttctcatttc cgtttccccc aggtgaatga cagatagccg
      361 aaccaacgac acacgatccc acggcagccg gaccagtgga gcgtcaggcc ccacgcatga
      421 caatccggca atagggaatg aggtgaaaca tttctttgat ttttacaatg agaggcgtct
      481 catgactgta caggttttcc atgtcatacc ccatcacaac tcatgccttt tgtggccagt
      541 gagcatttaa agagtgacaa gtccctgata cgcagatgta tgagaggaac aaacaaatgg
      601 atacccgatg ctggattata ctgaatacct aaaatttttt gtggcgctgg tagccgtcat
      661 caatcccttg ggggctattt ccatgttcat cgcgctcact ccaggaaaag agatgaaagc
      721 ccggcaaaac attgccagga tcgcttcatt ctcggcgact ctgatcctcc tggtgacctt
      781 ggtcgcaggg gaaggagtcc tcgcaatttt cggaatcagt gtgagttctt ttagagtggg
      841 tggcgggatc ctcattttac tcctggcgat ttccatgctt caggcacaac ggagcctcac
      901 ggtgcagacc aaagaagaag ctgaggagtc cgacaggaaa caggacattg ccatcgtccc
      961 gctctctacg ccgttactgg cgggacccgg ttcgttgagc accgtcgtgc ttcaggccca
     1021 taaaggaacc ggctgggctc atgaagcctt tattgccctt gcgatcgttg cagtaggcat
     1081 gtgcatttgg gcgtctttac atttggcacc gtggatctcc cggcatttag gccagaccgg
     1141 cattaatatt tttacacgta tcatgggact catcctggcc gccatcgccg tagaatttat
     1201 tgctaacgga ttgaaggggt tgtttccggc tttggcggga taagacaaaa agagtttggt
     1261 gcgtcggcat gaattcgcat gaggccaaag ccgactcgta tgttcgcagc tctaccccgc
     1321 tcgactcaac aggaaattgc gtgtggatcg ctctcaatcc actgcctgct ttaaaggcgt
     1381 tttgccgtaa ctgacccttt tgtgaattac cagaaactac ccatgattcg gtaggggttg
     1441 ccccatagga ttcatatgtg gggttagaac gctcttccgg gataggagga aggaacaagc
     1501 gaatgagagg tcgtattcgt ctcaatctcc atgagattcc atgaactcaa tacagacatg
     1561 aggaattccg gaatcttaag acgatcatgt ggtcgtcggt gagaggtcga acctaaaacc
     1621 attctgactg gagaaacggg tgaagccttt atcctgtaga taaagtccgg agagcgatat
     1681 gtagaccatt cgcgctctgc catgcccttc ccctgcagga cgtatctctc cactcactga
     1741 tggcgtgggc gaacacccaa acatggatac accaaaaatc cacaatatga tcaaggccca
     1801 tcttgaacca cgcatcattt ctcccttgaa aagaagacgt tccaattttc tttcacgcga
     1861 cagactctca atcagtccaa caacttcccc aatggggtaa cagacgtcaa aggaaaccgg
     1921 tgaacgggac tggaccaatg cctacctgat gaacgccggg ctctgggttg ttctgcgcat
     1981 agagctttgg ctcatggatt ttattcatcc atggcagccg gagatgggtc ttgctaccta
     2041 ccgtccttgc cgacccatac cgcattcttt tcccattgag ggagatggat cttccccggt
     2101 tggactttag gattgaatcc ttttggactt tcaggccagg tgcctttcgt gttgtttaag
     2161 ctgctcggtt tctcgatccc gtttttgccg tccagtgttg tttttttccg gttgttgaag
     2221 gtcacccgga gtctggagtt gatcacgtct ttaatcttca gaccaactaa aacctgcaac
     2281 ccccatcgat tggtacgact aatgagatca aaatgaaacc acagaacctg ttcctgggta
     2341 tcatacaaac acctcctttt cagaaacagc aaaggcgaca tggctttgac ctaacggcaa
     2401 tctaccaaca caaacagcga aaaaacattc tagtaaaccg ggccgtcatt cagaacaaaa
     2461 ggggaagatg tgcgaccgga tttcttgcaa cccggctgat gaacagacct cctggactag
     2521 gatgatagat ctttggccaa tatctcttgc tcggcagcta accaatcatc gagatcctgc
     2581 ccaggatgcc cgcctcggcc ctgaaataat tcatacgccc gccgggcaat ccgcgcgggt
     2641 ccatccccat tagcaggctg gaaattcaga gactcggtga cggcagcgat caggccctcc
     2701 tcaacctcta aaggtgccgg cttctttaaa ggctttttca ttaacatgcc cttgagcttg
     2761 ccagacgagg aagacttttg gcgcgtatca tttttggatg gttgaactaa tggctttttc
     2821 gccatcacgt cacctctacg tgaggaatgg catcggtagg agccaaggac ctttgtaaaa
     2881 tttcggaagg aaatgcaggt ttttttccga gagttttgag tgtatgggca atgcgttcgg
     2941 gttgtcccca atcactccac agcacttctt cgaattccat cactcccaga tgctcaggaa
     3001 tccgttgaag caacgtacga gaaaagtcca ggataggcat gtctcgataa atctcgtgca
     3061 gcgtctcatt ttcatgcacc gttccaatcg tcgctcctaa ctgttcaaac cgctccatca
     3121 tatccggcag ggttcgccaa ccaagtttcc agagagtggc cacttttgcc acaatcacca
     3181 aggtattcca aagagcgcca cttgccatga cctgcttccc ttcaaggagg tctggttttt
     3241 caagaaagga accaacgtgg cgaatacatg acccatggct ccagcccagg acttcgccca
     3301 ggttgatcca cccatagtcc aattcgggat gggaaggagg cacgccaaac aggatcaaac
     3361 gatcctggag aaagtgactg gcgcgaatcc ctcgcctgat cgtttcgaga aaacgagctt
     3421 caggaaagac aaaatgatcg gatgggaaaa tgacgactgt agcatcagca tcccatgctc
     3481 gaacaaaggt tagcggaagg aacactcctg gggctgttcc gcaattccgg ggctgaacaa
     3541 taacgtgccc agaggtactt ccctccatgg cttcatttaa aaacttccga tggttccgac
     3601 cgatcaccgt aaccttctgt tcaggtctga ccaatcggtc tgcccgatcc agcgtatgtt
     3661 ggagcatgga tcgatttccg acaaaggtac aatactgctt gggtttatgc ccgcccaacc
     3721 actgttcgat aaacgggcgg gtcctttccc cctccccacc agctaaaata atcgaccaca
     3781 acggatgagc cccggctctt agcatgttct tcatctccct ccctgggtca tgaatgccat
     3841 agcacaatag gggtcttccc ccattatgga atgtcctttg taaaacattt cacacatgga
     3901 aggaactagg gatttcccta gtcaaccagg aaaatgtccc tagggcctat aataaaagtc
     3961 caattcacca tgtcgggcat gacagggggg aggcattgcc gaaaatgcaa aaaagttaga
     4021 ggtcaatgga atggaatcgt attactttgc ctgtcatcct cgcaggtttg tagtggggat
     4081 ccatccggag aagacgtcaa aggtgaagtg tgaggcgtca ggggtcacaa tgggagatag
     4141 aaaaccagat ggactgccaa tgaccaatgt cgggagtgac aagatcaaac aggtgtccga
     4201 ttcaacatga tgggaatgct atccaaaatc cccacaaact cattcacggc cacccccctc
     4261 tgcggaatcc ggaggaagcc accttggcaa tgggtttgag cctctcaccc ctttacccaa
     4321 tgccaggcct cgtcggtctg gtctgatgtg aaatatttga attgagccct gggccaggga
     4381 acagaaacct ttggtccgcc ctccaaccat cgccggcttc ccaccaccgc aattcgttga
     4441 taatcctgaa cgttccgaag atgatgggtc atctcctccc ataaggcttg cccattttcg
     4501 ccttcaacgg ttgccatatc gcataagaaa ttaatcggcc caacttcctg catcagatgt
     4561 tccaggtacg cattcaagag atcatactct tcggctgata atttaccctc aatccgtatt
     4621 cccacacttc cgtcttcact ttcatctaaa atccgataca tactgtacct ctcaccctcg
     4681 agacacaaat tcgtgaccgg ccctcacgcc gtacttgcca cggaggaacc ccggttaatg
     4741 acttaatatc tttgatttcc aggctccact cacatctcct ttgccctaga agcatttctc
     4801 cttccctgac ggtatagact cgctattctt tctctagcga gggttatgcc aggagagggc
     4861 ctcgaaccaa ttctctctgt tcgcaatggg ttttgaggaa acggaccagg ccgggaaagg
     4921 tctatttgac caaaaaagct acagggatta atggggaact gttgaagatt gcaacaacaa
     4981 ggggggggaa agcgtaaatc agacccattg gtcattccca gagttgatta tgaggaaccc
     5041 agacggaatt gagtcattcg aagatggatt ccccctcagg atccgcgggg atgtccgagc
     5101 taagttgaca ccctgatacc atgtatcgac aactaagtcc catcgggacc atccctaacc
     5161 ctatatttcg gaaatccttt aagtcttcat ttggggtggg tgtctgctga cctatgctcg
     5221 ataacggggc aaggccggct gtcccagatt tcgctctatg gttcaatatt ctcccgtgtt
     5281 tcttcagcca tgacctcttg ctccgcgagg aaccaatcat cgaggtcctg gccgtggtgt
     5341 cctccccgcc gctggtagag ctcataggcc cgttcagcaa ttcgtggata aataccctca
     5401 ttcgcgaatg aagtctcgcc atcatgttcc tgttccggcc gaagaggttt gcggttcttg
     5461 cccctggaag atgtcttggc gcgtggctgc ttatccgtca ttctcctgct ttgacgagtt
     5521 gcgccagacg gttcctccgg ttccttgcgc tcacttgttg ctaatacgcg ttgacgactc
     5581 atcgggtcac ctcgagtgaa gagagtgcat gaggggaggt acagggcatg gtgacaagct
     5641 cagaggggaa cgtaggctcc ttaccaagaa cattcagcgt gtcacgaatg cggtctgagc
     5701 cttcccaatc actccaaagc acgtcatcta gctcaatcat ccccaggtgt tccggaacac
     5761 gctggagtaa ttcggaagac acatctaata tgggcatttt ctgatataac tgacgaagca
     5821 tacgaccctc atgagtgctc ccgatagctt ttcctaatcg ctcaaaccgt tccatgatga
     5881 ccggaaggta ttgccagcca agcttccaga tggtggaaac tttcccgacg atcaccgacg
     5941 tgttccagag ggctcctttg gccatggcat tgagggcctg gacatggtcg agttgctcaa
     6001 taaaggaatc gacttgccga acacaggatc ctccactcca acccaacacc ccccccacat
     6061 tcatccagcc atagtccaat tcaagatgag tgggacgtac ccccaacacc aggaggcgat
     6121 cctgaagaat acgactcccg cctatggcac gccgaaccat ttccagaaaa cgattttccg
     6181 gaaacacaaa atggtcagaa gggaagatga ccaccgtggc acttggatcc catgcccgga
     6241 cataggtgag agggagaaac atctcagggg cggtgccgca atagtttggt tcaagaatca
     6301 cttgtcctgc ccgctgacct cctaaggcct cacctgcaaa cccctgatgg ctttgggcca
     6361 ctaccgtgat tttttgatga ggagccccaa ggcgatccgc ccggtctaag gtgtgttgga
     6421 gcatcgaccg gtttcccaca aatgtacaat actgtttggg tttgggatag cctaaccatc
     6481 gttcaataaa cggacgcgtt cgttccccct ccccaccggc gagaataatg gaccataagg
     6541 catgggattg agacataggg cggagctcct tcctttgcac gaaaaaattg tatgaaagct
     6601 atgaataaat tatgaataaa tctacgcgga cctttatatc gtgaaaaaaa agggagggat
     6661 actgggtatt tccctaggaa accaagaaaa tattcctagg gttctgaggg atatccgaaa
     6721 caacataccg agcctatcct tcctccttag tcatccccgc cgtcgccagc ggagccccat
     6781 tttctggaga aattaagaaa aaaaggagtc ttgattgaaa atgtcagaat tcccgcgtgg
     6841 ggatgaggac ccgagtaaaa ctgtcgaacc aagcaaacag gctttaggca gtcaattttg
     6901 gcctttcaga atcctcggac aaaatctccc ttgcggcttt caaccaactg gccaaactcg
     6961 ggccacgagg ctcacctccc tgctgggaga tctcctgggc acgattatca atgatgatcg
     7021 ctagcgaatt agaagaacca tcattcctca agtccgggtt ttcattgaca gaaaccggca
     7081 gattttgcat ttctccctgc ttcttggaca caaataaaat atctttcatt ggatcaacct
     7141 ccgccttgta ttgccttggt attagcccag ctccatatca tctcaagaca gatgagcaga
     7201 atacggacgt gctctttcct gcgacgatca aaccggattg aacgctcacg ggcgaagaca
     7261 tatcaaatcg ttcaaaaccg cctgtgaaca gtggcgacca acaccgcgcc ccttggcaca
     7321 ttcagcgtcg taaagcacga gaagaatcaa aggccttcac cttccttctt taggacccag
     7381 gggatgacat tcatctccat cgatttcagg cttccaccgg attaaggatg acctcaccac
     7441 attcccgaca aaccagatgc cccgttttct cgccttgtat gttgacctgt tcgtctacca
     7501 ggcgttgatg ggcgcagact tctttcctcc cggttttaat ggttccattc acacgaccgg
     7561 ccttatggtc attcatacgt ccccccttct gaaaaatgcc caatacctta tttgaaaatc
     7621 catcacctct aaccggaatt ccgtcttcca tcaagtcaca tggaagcgag aggctgccac
     7681 tgccttgacc ccatcgtttt gcaagatctt acggcaggct tgttgaacag aagcgagatg
     7741 aaacggcttg aggaaaaatc cttgagcacc ttcctgtaac attcgccgga gcaatggttc
     7801 ctccgtcgcg cccgacatca tgagcaccgg catctgatgt cccagccatc gtagttcatc
     7861 cagcatggtc cgcccatcca taatgggcat gtgcatatcc aataaaattc cattcacgca
     7921 acggtcgccc aacagatcca ggccctcccg tccatttctg gcaacaagca cctcatatcc
     7981 ccattcctga agggcttgat ccaacaaccc ggctacggca ggatcatcat ccaccaccaa
     8041 caaacaggga cggacttcct gagtctgagc ctcggtcttg gcacgcgaca catccggcga
     8101 tgtggtttgg agcccttgaa gaatttccag acaggaaggg caataggttt gtgcaggctc
     8161 gatatcggag aatgtgagtt gatgcctgga caaataggca tccaaaactt gccaggcatc
     8221 tgttccgacc cgcccctcag agggatcatg aacccgttca caccaggcac aggaaaacac
     8281 gaattgggag gtaggcgaag atctcaggag gctatgccgt aagacatctc cccgggaaat
     8341 gactcccaca agctgtcgat cacgaaccac aggcatcctc agaacttggc tgtgaaccat
     8401 caccttcatc acgacatcga gcggatcctg ctcacacaca tataccggcg tggtcatggt
     8461 gtcttcgacc ctcagttcat ccagggaatt cacgcgtgaa caggcttgta acaggcggaa
     8521 ttcggtcacg acccccagga tttctctgct ggaatcgacc accggccatc cgctgtattg
     8581 accggaaaga aattgcaaag ccagaccctt ctcaaaacga ccgggtcgaa gaggaacggc
     8641 ggcggtgttc atcagatctt ccgccaacgg agaagatgaa aaaaggtggt tcatccgggg
     8701 gcctctcttt cacatcggtt tcaaccatga aaccatcaga gtacaggggg attttgccgc
     8761 taaggtgagg gtgtaaaata ccgatcgacg taaaaaaaaa gaactaggga aattcctagt
     8821 cctttcggga aataatccta ggatgaagtg aggagaggtg agcggaatcg tctacttctc
     8881 aaccaggccg caagccaccg caaactgggt caactctgga atcgtatgca tccctaaagt
     8941 ttccatgaac ttggctttat gaaattccac cgtttttggg gaaatattga gaagggtggc
     9001 tatggctttg gtggaacgac cttcggcgat caactgcaag acttcccgtt gacgaggagt
     9061 gagctggttc aacgatggta ttcccattcg tggtgagtcc cgagggaaaa tatcccctcg
     9121 caacactaat ggggtgacat acttgtgacc tgccaacacc gattgcacag cctgcttgag
     9181 ttctgatccg gcagaccgct ttaaaaggta gccggaagcc ccagcctcaa acgccgcagt
     9241 tatataggag gaatccccat gcatcgatag aaaaataatt ttacagtgag gaataagttt
     9301 tgttagacag cgagcggatt ccagaccgtt taaacttggc atggaaatat ccatcaccac
     9361 cacatccggg ttcagatgcg gaacgatatc aagcaacgct cgtccatctt ccacctttcc
     9421 cacgacctca gcgaactcct ccacaagctt ggccacccct tccaggacca tgggatgatc
     9481 atccgccaac acaatacggg gaaggttcct tgaggatttc ccggcacacg agcttggata
     9541 aggatgtgag atcggcaccg gaaacttgtc ccttcaaaaa attgataccc gtctgaatga
     9601 ggctggtact atatccaatc gcaggcaaat caacactagg aaaatcccta gttcccgaaa
     9661 aaaatatccc tggtttaaaa aattcgcatt tttttattcc ggagacacga gaccatgggt
     9721 aatggcatac ctcgttaatt cggccgtgtt ttgacaattc aactcccgga taatcttggc
     9781 tcgatgaaac tcaatggttt ttggagatac atgtaacatc ctcgccatct ctttggtgct
     9841 gtggccttcg gcaatgagtt gcagcacttc gcgctgtcga ggcgtgagac agttaccctc
     9901 agcttgaggg gccgacattt cctgggtgaa cgtacccaga aaatccttgg caatggccgg
     9961 agtcacatag tactgccctt tcattacggc attgatcgct tgcatcaact ctgaagccgc
    10021 agaacgtttt aggagaaagc cggaggcccc gatttgaaag gcttccttgg catacagagg
    10081 atccccatgc atggtcagaa aaatcagctt gaccgatgga agtaatttct tcagttgacg
    10141 ggcggcatcc agtccattca attttggcat ggaaatgtct aagacgatga catccggttg
    10201 caaattcgga gcaacctgaa gaaggacccg cccatcctcc actttcccga caaggtcaca
    10261 gtcctccgtg accagtttgg ccaggccctc cagaaccaat acatggtcat cagctaataa
    10321 tacacgaggt cgggtcacca tggaaaaggc cctatgtcgt tctgcttttt gtttaatcgt
    10381 ataggcattc gttggtacct ctcatcgaat agcgtgatcc aaaatcctcc tccggacgtc
    10441 actcccgaca ttccttatcg ggagcctctc cctcagttcc cgtcaccccc gacagtcgtt
    10501 attgggagtc catcttactt tcgtttcgaa tggatccccg cttactcatt gcggggatga
    10561 cagatgaagg agaggatcgg tcattcccga cggtttgcag cgggcatccc tcctgatttc
    10621 tgtctcccca ttgttgccca taactcctaa tgtctcacga ctgggcgtct acaggaatgg
    10681 agaccctcac agttgtgcca tagccaggct gactctccac caacagggtt ccacccacca
    10741 gacgaacccg ttctctcatg ctgactaatc ccaaacctcg ggaaagaagc ccgtccactt
    10801 caaatccttt tccattgtcc tgaattgaga gcgtgatttc ctgctcgtcc tccctcaaaa
    10861 tgagatggac tgccgacgcc tgagcatgct ttgaaacatt tcgcaaactc tcctggacca
    10921 cacggtacag acatgggccc accgcttggg aaattattcg tgctccatca ggcaactcgc
    10981 acgtgaccga gattccttcc cacttctcaa agtcttcgca caacgagcga agagccgagg
    11041 ccaaccccag atcatcgaga atcgacggat gatattggta agctaaatgc cggatatcat
    11101 c
//
LOCUS       DATIAL010000130         9848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1205444, whole genome shotgun sequence.
ACCESSION   DATIAL010000130 DATIAL010000000
VERSION     DATIAL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205444"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VLA60_04105"
     CDS             <1..796
                     /locus_tag="VLA60_04105"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSF08572.1"
                     /translation="RVCIHCGRNIVTGRTEKMSKSKKNIASPEDLCERYGADTARMFS
                     LFAAPPEKDLEWSDTGVEGCYRFLNRVWRLVQDLLPVVNGVAANPGSGESVLLPQLER
                     ATHQTIKKVTEDFERGFQFNTAIAALMEFVNELYKFWKEFPGEALTAGERAEWRTTLE
                     TLVVLLAPFAPHVTEELWELLGKRPGMSRQAWPDFDPVLVASAEWTIPLQVNGKLRSK
                     IVVPAGSTREQIIAGAQADPKLVEWLQGKVPRKIIYVEQKLVNFVI"
     gene            786..1511
                     /gene="lptE"
                     /locus_tag="VLA60_04110"
     CDS             786..1511
                     /gene="lptE"
                     /locus_tag="VLA60_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HSF08573.1"
                     /translation="MSSKSGRPGWLFQRVRWLISGWGILLCLTAGCGYQFTVEGPGPV
                     IGGSAGHVVQGPPIRLVFPVLKNNSFEPNLEFKYTRYLRQALQSVGSAEFVDDASADF
                     VMEGAILSVGLSSLAFTRTQTQESRVMVKVLLKVKDRKTGKVRWSQTGTSTAEFFVNA
                     TSTSDSERGLQFNRVLQDRAVEQAGQQVAANLADQFLGARERGVFDRNGKEIIPDPES
                     GGDESPQISAPSGDLVPPDSQMP"
     gene            1561..2652
                     /gene="holA"
                     /locus_tag="VLA60_04115"
     CDS             1561..2652
                     /gene="holA"
                     /locus_tag="VLA60_04115"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HSF08574.1"
                     /translation="MKVHELQSQINRHGLSPLYLVIGEEPYFRDQALSILRAAGQETD
                     PSQSSDHSTAHDSSQMFHVDVVYGDETDASEILAISEEASFFSLRRLLIIKWADKLSA
                     RDGESLMPYFQTPNETTTMVITAAKLDGRTKWVQELKKRGTVVECAPLFEGQRAGWVT
                     QRARELGLQLEDSALEILKDQVAEGLYGTAGELEKLVAFMPEGHPVRAQDVETIRGKP
                     PGISVFDWSEAVARGDQGRALDIVAKNLETGEAPLRMLGAFLWQMRKIWKTHALMQEG
                     HDAGQAARQAGIPPFRAREFIQQVQRWKQSHLRRAWELFAQADSALKGGRASRPKLIL
                     DDLVIQLCRATKAGQGSKASGVRMKSHKA"
     gene            complement(2677..2949)
                     /gene="rpsT"
                     /locus_tag="VLA60_04120"
     CDS             complement(2677..2949)
                     /gene="rpsT"
                     /locus_tag="VLA60_04120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HSF08575.1"
                     /translation="MANRHHSAIKAARQTLKRQERNRSILRRVRTFIKKVHGAVKENK
                     GDEAKASLRGATSELHKAVTKGTLHRNTASRWVSRLASRVNALSPK"
     gene            3185..3261
                     /locus_tag="VLA60_04125"
     tRNA            3185..3261
                     /locus_tag="VLA60_04125"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3219..3221,aa:His,seq:gtg)
     gene            3543..3860
                     /locus_tag="VLA60_04130"
     CDS             3543..3860
                     /locus_tag="VLA60_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08576.1"
                     /translation="MRNSMKYPEGATPLDPDEMDGLRHKHVTTREELDCFEQANIQKG
                     LQWMKRSRQADIIQESYMRELYRQLFWGGLGLGWNISYHRKKYRSRSGGLRVPFGIGR
                     GKN"
     gene            complement(4043..4519)
                     /locus_tag="VLA60_04135"
     CDS             complement(4043..4519)
                     /locus_tag="VLA60_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08577.1"
                     /translation="MQASVGGIDYKDVNFPESEKNFDFTQDGKHFIRKVKVCGGHAGL
                     LPQQAHSHQMWEIKGVKIMDSEVKLEDSPALNPSVIELQNIEKFTRANCLDKVEQAGE
                     NKPGWSEWSECFTGQVATGIRAYYGKDKYVTGLKLHCQSVASKRIEAFPVKDNIGY"
     gene            complement(4534..4974)
                     /locus_tag="VLA60_04140"
     CDS             complement(4534..4974)
                     /locus_tag="VLA60_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08578.1"
                     /translation="MISFNRVTGVSTWVWVTAAVGTLSFVSLVFGETAIRSDYDKDDY
                     ILYMATVIQNDGSKPGLTDDTATGTTSQAVRQGVGETSGLSGSQGEASVEVNVGKNKK
                     LPNPTNYYAITGLGLEERKNNPCLLKLYGTLVDPRYQGGIRVVA"
     gene            5563..7257
                     /locus_tag="VLA60_04145"
     CDS             5563..7257
                     /locus_tag="VLA60_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSF08579.1"
                     /translation="MTTAVFIPLLPLLAAMLIGWQGKRLASRVALIGIVATAGSSVFS
                     FLTLYHVSTEGPFQLTFWSWGHGASPSLTFGMLADRLTAVMMVLISSVSMVIHLYSRR
                     YLQGDAGYQRFFGLLGLMTFTILSLVASANFLMLFLFWQLLSWVLYLLLAFNFSYPAS
                     YEYARKTLLVHRIGDISFLCGLLLIYQYFGTFEFAAVFQLAAIDPPMIRLWSGFPFEI
                     SIVPVIGLLIFVGAMAKSAQFPLHVWLPDTMDSPTPVSALMHAGIINAGGFLLNRLAP
                     FYALSPSTLHLVFIVGVCTVLLGASMMLVQNDIKKTLGFSTMGQMGYMIMECGLGAFA
                     LAIFHLIAHGFFKATLFLASGSIIQAARHEPKFPPTSGHRPTRLPTTLTWTTGLSVTL
                     LMPLFILLVAHGVLDVPLRDDHGAVIFLFFGWVTASQAIFSLYRLQAVASWKVAGAMI
                     ATLFFIGFIYLWAGESFTHFLYPEPGVANGFFEAAAFPPQVFDALIIMSTLVVLFGWL
                     IMYTTARGQKILNPHWVLSIRQTGWVLLINRCYVDGLYKKLGAALLRLAEKVAYRY"
     gene            7274..8512
                     /locus_tag="VLA60_04150"
     CDS             7274..8512
                     /locus_tag="VLA60_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08580.1"
                     /translation="MSPKNNHYLLLSAVPGLLMPENTTGRNGSSALWGIGIATILVAA
                     GVLGLLGWNTASDPSFSFLAAAILLACFCGILGQKGSIGGSGDVAPIILLGGLGLGGV
                     INVEPVNRLFLIGLLGYAGFFLARHMAASLQKSLALVHVSLAIMLGVLAAFMEERVPI
                     VGVLFLLITFLPLVPFHLPFLGIVRSSRESLAGVWVVVWLASGLSRLKDLEGLLPLDG
                     MEVFPVLALGSALYTSLKAMGHRLPREGIAYATITLLALLWGLIGELTHTAVWGIPFG
                     IAVALLMSAMLCSYAFLSERYGTHNLSTLQGLGSGLPRFRGVFTFLISLIMLLPVLPM
                     VGGFRSLPPDVSGSHALLPVWLLVFTVWLSISWIFTTLLHRTAFGKPRSDVPDWDLSF
                     YELWTLFLLMGMAGLLGILA"
     gene            8555..>9848
                     /locus_tag="VLA60_04155"
     CDS             8555..>9848
                     /locus_tag="VLA60_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HSF08581.1"
                     /translation="MTVATVQEVQSFKDIERIEVRSLIHLAGESISQFWPMQTFIHHN
                     PLHGLEHLHIHEAIAQAGTFLGGRGYLSNREFRDLYRQGRILEDAITDAVAPFAGNHF
                     VTIGTRNISHLEILRTLLIHGTGTVPADVTDAVFLKACEDPHVTALVTGLQSRGTHRD
                     AHLSLRQQADQARQNLATQQTLAAWCDHMCETSIQEEINQELIKWSAGFLDQGHAPWP
                     MPMREQTFYGGWKILAQQDGAGFLQGIPDWKRKLQQLPDRSEDAVLVCLQALGLPQSV
                     WSNVFTLHLAQLPGWTGFLKWRADQVDHEWQNAFPIDLVQYLAVRLFYERELVQRVCH
                     HRFGIPGTYPAIVSSLEDFSFGFGLYHAFRTGRLNLEILPALDEALFTTIPLPLQTLD
                     ALGREADAKWGTVQQDLTAKGHTLMLCHLAGSLGLALKD"
BASE COUNT         2051 a         2447 c         2716 g         2634 t
ORIGIN      
        1 gcgggtctgt attcactgtg ggcggaacat tgtgacaggc cgtactgaga aaatgtccaa
       61 gtctaaaaag aatattgctt cccctgagga cttgtgcgag cggtatgggg cggatacggc
      121 cagaatgttt tcgttatttg ccgcaccacc ggaaaaggat ttggaatgga gcgataccgg
      181 agtggaaggg tgttaccggt ttctcaaccg ggtctggcga ttggtgcagg atctccttcc
      241 ggtcgtgaat ggggtggctg ccaatccagg gtccggcgag tcggtgcttc tgcctcaatt
      301 ggaacgggcg acgcatcaga cgattaaaaa ggtcacagaa gattttgagc ggggctttca
      361 atttaacacg gccattgcag ctcttatgga atttgtgaat gagttgtata agttctggaa
      421 agaatttccg ggggaggcct taactgccgg cgagcgagcg gagtggcgaa caacgttgga
      481 aacgttagtg gtgttgctgg caccctttgc tccgcacgtg actgaggagt tgtgggaact
      541 cttagggaag cgcccaggga tgagccggca ggcgtggccc gactttgatc ccgttctggt
      601 ggccagtgcc gaatggacga ttcctctaca ggtgaatgga aaacttcgga gtaagattgt
      661 ggttccagcc ggttcgacaa gggagcagat cattgcaggc gctcaggcgg atcctaaatt
      721 ggtggaatgg ttacaaggca aggtccctcg taaaattatc tatgtggaac aaaagctggt
      781 gaattttgtc atctaagtca ggccgaccgg gttggctctt tcagcgggta agatggctga
      841 taagcggctg gggaatcttg ctgtgcctga ctgccggatg cgggtatcaa ttcacggttg
      901 aggggccggg cccggtcatc ggggggagtg ccggccatgt ggtgcaagga ccgccgattc
      961 gtctggtctt tcctgtgctc aaaaataaca gctttgaacc aaacctggaa ttcaaataca
     1021 ccagatatct tcgccaggcg ttgcaatcgg tcggaagcgc cgaatttgtc gatgatgcct
     1081 ccgccgattt tgtgatggaa ggcgcgatcc tatcggtcgg gctttcctcg ttagccttta
     1141 ctcgcacgca gacacaagaa agccgggtta tggtgaaagt gttgttgaag gtgaaggatc
     1201 ggaaaacggg gaaagtccga tggtctcaaa ccggaaccag taccgcggaa ttttttgtga
     1261 atgctacatc cacgtcggat tccgagaggg gattgcaatt taaccgggtt ttgcaggacc
     1321 gggcggttga acaagcgggg caacaggtgg ctgccaattt agccgatcaa tttttggggg
     1381 ccagggaacg gggggttttt gacaggaatg gaaaagaaat tatcccggat ccggaatccg
     1441 gtggtgacga atccccccag atctcagcgc cttccggaga tctcgtgcct ccagattccc
     1501 aaatgccttg attacctgaa tggtttggca ggattgtctg ctttttgagg cgtattcacc
     1561 gtgaaagttc atgaacttca aagtcagatc aaccggcatg ggttatcccc tctgtatttg
     1621 gtgattgggg aagagcccta ttttcgcgat caggccttga gcattcttcg tgcggcaggt
     1681 caggaaacgg atccttcaca gtcatcggac cattcaacgg cccatgattc ctcccagatg
     1741 tttcatgtcg atgtcgtcta tggcgatgaa accgacgcgt ccgaaatatt agccatttcg
     1801 gaagaggcct cgtttttttc attgcgacga ctcctcatca taaaatgggc agataaactc
     1861 tctgctcgag atggggaatc gctcatgccc tattttcaga ctcccaatga aacgactacg
     1921 atggttatca ctgcagcaaa attagacggg cggacgaagt gggtgcaaga gcttaagaag
     1981 cgggggacgg tggtggaatg cgctccccta tttgaggggc agcgagcagg gtgggtcacg
     2041 caacgagcgc gagagttagg cttgcagttg gaagacagtg cccttgagat attgaaggat
     2101 caggttgccg aagggttgta tgggacggcc ggtgaattag agaaattggt ggcgtttatg
     2161 ccggaagggc acccagttcg ggcacaggac gtggaaacga tcagaggaaa accgccgggt
     2221 atttcggtgt ttgattggtc ggaagccgtt gcgcgagggg accaggggcg ggcattggat
     2281 atcgtggcca aaaatttaga aaccggcgaa gcacccttac ggatgttggg tgcctttctg
     2341 tggcagatgc ggaagatctg gaaaacgcac gcgttgatgc aagaagggca tgatgcggga
     2401 caggccgcac gccaagcagg cattcctcca tttcgtgcgc gggaatttat ccagcaggtg
     2461 cagcggtgga agcaatccca cctccgcagg gcatgggaat tattcgctca agcggattcg
     2521 gctctaaaag gagggcgggc atcacgtccg aagctcatcc tggatgatct ggtcatccag
     2581 ctttgccgag ctactaaagc tggtcaaggc agcaaggcct caggtgttcg catgaaatct
     2641 cacaaggcgt gagaaccctt aacccttttg accgtattat ttgggtgaga gagcgttgac
     2701 gcgggatgcg agacgggaaa cccaacgaga ggcggtgttc cgatgaagcg ttcctttggt
     2761 tacggcttta tgcagctcag aggtcgctcc ccgcaaagat gccttagcct cgtcgccttt
     2821 attctctttg accgctccat gcaccttctt gataaaggtc ctgacgcgtc gcaggataga
     2881 ccgattgcgt tcctggcgct tcagagtttg acgagctgct ttgattgctg aatgatgtcg
     2941 attagccata ttttcctcca gaaaaaatcg aagtgtggtt tttaacacaa ttttatgaaa
     3001 gtggtcaatt gaacgcgggg taaattgttg tggtgtttaa aaggctttat ggcccttgac
     3061 atatgaggtt ctttcgagaa ttaggtcaat catcctcgat tgtcgtaatc ggtatgtggc
     3121 ttgcgacacc tatggccctc tgataagatt gccctcttct tccgctcgaa acttccatgt
     3181 gatggtgggt gtagctcagt tggttagagc gccggattgt ggatccggag gtcgcgggtt
     3241 cgaaacccgt cactcacccc atcttcctct ttcaaaaaac gctacaatct gaaagactta
     3301 gtatggcagc acgccttgac ggtgatgcat tggttgcgga gattaaggcc atattagagg
     3361 atctattttg tccgcataaa gttgggctta agcgaaccat tcatcgactt ttgggttggg
     3421 ttcactcgat ttcatccttc gatccatggt ttccttgagt gcggctagat aggctcggat
     3481 ttgttgagcc ttctaaccaa tgaaatcctt aaggatcctc ctcccgactt ttggaataat
     3541 gaatgcggaa ttcgatgaag tatccagaag gggcaacgcc ccttgatccg gatgaaatgg
     3601 atggactccg acacaaacat gtcacaacac gggaagagct ggattgcttt gagcaggcga
     3661 atattcaaaa aggtttgcaa tggatgaaaa gaagccgtca agcagacatt attcaggaaa
     3721 gctatatgcg tgagctttat cgccaattgt tttggggagg tttggggctg ggctggaaca
     3781 tttcgtacca caggaaaaaa tatcggagtc gatcaggggg attacgagtc ccttttggaa
     3841 ttgggagggg taaaaactga gagatcacaa aattaattca caggagagtg taaaaatttc
     3901 gacttttccc tctcaggcta aacctaattt ccatccagga ttttcctgaa agcgatccac
     3961 tccagaataa caaccagatg gagtggcagg tttccaggtc ggtcattttt caatggcgcc
     4021 ggctttgccg ttcacttcca ctctaatagc caatattgtc cttgacggga aaagcctcta
     4081 tccgcttgga agcgacgctt tggcaatgaa gttttagacc ggtaacgtat ttgtcttttc
     4141 cgtaatacgc gcgaataccc gttgcaacct gtcccgtgaa gcattctgac cactcactcc
     4201 agcccggttt attttctcct gcctgttcaa ctttgtcgag acagttggcc cgggtaaatt
     4261 tctctatgtt ctgaagctcg atgacggacg gatttaatgc aggcgaatct tcgagtttga
     4321 cttctgagtc catgattttg actcccttga tttcccacat ttggtggcta tgggcctgtt
     4381 gaggaagcag tccggcatgc ccgccacaca ccttcacttt acggataaaa tgtttgccat
     4441 cttgggtaaa gtcaaaattc ttttcgctct ccggaaagtt gacgtctttg tagtcaatcc
     4501 ccccaacaga tgcttgcacc gctcaagttc ctgttacgca accactctta ttcctccctg
     4561 gtaacgggga tccaccaatg tcccatacag cttcaggaga caggggttgt ttttgcgttc
     4621 ctcaaggcct agccctgtaa tggcatagta gttcgtcgga ttcggcagtt ttttgttctt
     4681 tccaacgttc acttccaccg atgcttctcc ttgagaaccg gatagcccgc tggtctcgcc
     4741 cactccctgt ctgacagctt ggctggtggt gcctgttgcc gtgtcatccg ttaaccctgg
     4801 cttgctgcca tcattttgga tgacagtggc catatagaga atgtaatcgt ctttatcata
     4861 gtcagagcgg attgcagtct ctccaaacac caatgaaaca aagctcagtg tacccactgc
     4921 ggccgtcacc cacacccagg tgcttacacc cgttacccga ttgaatgaaa tcatgatcaa
     4981 cctccttgtt ctgctcatca actaagcggg gttatacaga cacttaaatg ctttacccac
     5041 cgtacgattg ctcaatatta taaacctctt aaggggctat gccctcccgc aggctcaatt
     5101 cttcggtagg catgtcccgc agtactgtgg cgcaattctt caagaaattg attcccgggc
     5161 atgctactca ttctcattct gaaaatcgga agcccataga atttcgttca gaatgaaaac
     5221 ggtttcatta aagattttca gcataattag ccagagaggc cccttctcct ttctgttaaa
     5281 tgcatcgggc cttgaatttc cgtccttctg atcggacatg tggatcgcgc ggttcagcgg
     5341 gcatgatttt ctctccccaa aggcaccaaa atgtgtgaac ccgaaaggcc catgagttgg
     5401 gtctaacaca ttattgctta tcgcaatatc aatcgttaag taaaaggatt atgcttgatc
     5461 ggaacgggat acgattttaa ttacaggaat ttccgtctcg gcgattgtgc tatcgggatg
     5521 ttcccgatgt cgcctcgctt ttgagactgt tgttgactcc atatgaccac agccgttttc
     5581 attcctctcc ttccgctgct ggccgccatg ctgattggtt ggcagggtaa acgtctggcg
     5641 tcccgcgtgg cgttgatcgg gattgtcgcc accgcagggt ccagcgtttt ctcgtttttg
     5701 accctgtacc acgtcagtac cgaaggcccg tttcaactca ccttctggtc gtgggggcat
     5761 ggagcctcgc cttctctgac attcgggatg ttggccgacc ggctgacggc cgtgatgatg
     5821 gtgctcatct ccagtgtcag tatggtcatt catctatatt cccgacgcta tttacagggt
     5881 gatgcggggt accagcggtt tttcgggtta ctcgggttga tgaccttcac catcctctcc
     5941 ttggtcgcca gtgccaattt tctgatgttg tttttgttct ggcaactcct gagctgggtg
     6001 ttgtatctcc tgttggcctt caatttttct tatcccgcgt catatgagta tgcgcgaaaa
     6061 acgttattgg ttcatcgaat cggggatatc agttttctct gtggcctcct gcttatttat
     6121 cagtatttcg gcacgttcga atttgcggcc gtgtttcaac tggcggctat agatcctcct
     6181 atgatccggt tgtggtccgg gttccccttt gaaatcagca tcgttccggt catcggactg
     6241 ctgattttcg tcggggcgat ggccaaatcc gcgcaatttc cattgcatgt ctggctcccg
     6301 gacacgatgg actccccgac gccggtctcc gccctcatgc atgccgggat catcaacgcc
     6361 ggtgggttcc tgctgaaccg gttggccccg ttctacgccc tgtctccctc gactctccat
     6421 ctggtgttca tcgtgggagt ctgcactgtt ctgctcggag cctcgatgat gttggttcag
     6481 aatgacatta agaaaaccct gggtttttcc accatgggac agatggggta catgatcatg
     6541 gaatgtgggc tgggggcctt tgccctggcc atcttccacc tcattgccca tggattcttc
     6601 aaggccaccc tctttctggc ttcgggaagt atcattcagg ccgcccgaca cgaaccgaaa
     6661 tttccaccga cctccggtca tcgaccgacg aggctgccga ccacgttaac ctggacaacc
     6721 gggctgagcg tgacgctgct tatgccgttg tttattcttt tggtggccca tggcgtgttg
     6781 gacgtgcctc ttcgagatga tcacggggcg gtgatctttc tctttttcgg atgggtaacg
     6841 gcctcccagg cgattttcag cctttatcgt ctgcaggccg tggcttcctg gaaggttgcc
     6901 ggtgcaatga tcgcgacgtt gttttttatc ggattcatct atttatgggc gggtgaatcc
     6961 ttcacgcatt ttctttatcc ggagccgggc gtggccaatg ggtttttcga ggccgcggcc
     7021 ttcccgcccc aggtattcga tgcgctgatc attatgtcca cgctggtggt gctgttcggt
     7081 tggctgatta tgtataccac cgcccgtgga caaaaaattc tcaaccctca ttgggtgctc
     7141 tcgatacggc agaccggttg ggttttgctc atcaaccggt gttatgtaga tggactgtat
     7201 aaaaaactgg gtgctgccct gctccgcttg gcggagaaag tggcctaccg gtattagctt
     7261 tggtcagatg agcatgtctc ctaaaaataa ccactatctg ctcctctctg ccgttccggg
     7321 tcttctgatg ccagaaaata ccacggggcg aaacgggtct tcggctttgt gggggatcgg
     7381 aatagctacc attttggtgg ccgccggagt cctcggcttg ctcggatgga acacggcatc
     7441 cgatccctct ttcagtttcc tggccgccgc tattctgctt gcctgtttct gcggcatctt
     7501 gggtcaaaaa ggctcaatag gaggctccgg tgacgtggcg cccatcattc tgctgggcgg
     7561 actgggcctg gggggagtga ttaatgtgga gccggtcaac cggctattcc tgatcggcct
     7621 tctcggttat gccggctttt ttctggcccg gcacatggcc gcctcgcttc aaaagagcct
     7681 ggccctggtg catgtgtcgt tagccataat gctcggtgtc ctggcggcct tcatggagga
     7741 acgcgttccc attgttggag tgttgtttct gttgatcacc tttttgccgc tggttccttt
     7801 tcatctgcca tttttgggaa tcgtccggtc ttcccgggaa tcgctcgccg gtgtctgggt
     7861 cgtggtctgg ctggcttcgg ggctgtcccg gctcaaggac ctggaaggcc tgcttccttt
     7921 ggatgggatg gaggttttcc cggtgcttgc gctggggagc gccctctata cctccctgaa
     7981 agccatggga catcgtcttc ctcgtgaggg aatcgcctat gccaccatca ccctcctcgc
     8041 attactgtgg gggttgatcg gtgagttgac ccacactgcc gtctggggaa ttccattcgg
     8101 gatcgcggtc gcattgttga tgagtgcgat gttgtgttcg tatgcctttt tatccgaacg
     8161 atacgggaca cataatctga gcacgctgca agggttgggt tcggggttgc cgcgttttcg
     8221 cggagtcttt acgttcctaa tctctctcat catgctgtta ccggtgctgc cgatggttgg
     8281 cgggttcaga tccctcccgc cagacgtttc cgggtcacac gccctgcttc cggtttggtt
     8341 gttggtgttt acggtgtggc tctcaatcag ttggatcttt accacgctgc tgcatcggac
     8401 cgcgttcgga aaaccccgat ccgatgttcc cgattgggat ttatcgtttt atgaactgtg
     8461 gacgctattt ctgttgatgg gcatggcagg tttgcttgga attctggctt aaccttgttg
     8521 agcgaatgat gcaacacacc agaggatgcg gaagatgacg gttgccacgg tgcaggaagt
     8581 tcagtcattt aaggacatcg agcgcattga ggtcagatcg ctgattcatt tggcggggga
     8641 atcgatttct cagttctggc caatgcagac tttcattcat cacaatcccc tgcatggcct
     8701 ggaacatttg catatccacg aggcgattgc gcaggccgga acctttctgg gggggcgggg
     8761 ctatttatcc aatcgggaat tccgtgacct ctaccggcag ggccggatct tggaagacgc
     8821 gatcaccgat gcggtcgcgc cattcgccgg gaatcatttt gtcacgatcg gcacccggaa
     8881 catttcccac ctcgaaattt tgcggacgct cctgattcat gggaccggca cagtgcctgc
     8941 tgatgtcacg gatgcggtgt ttctcaaggc gtgtgaagac ccacatgtga cggcgttagt
     9001 caccggcctt cagtcccgtg gcacccatcg ggacgctcac ctgtcattgc gtcaacaggc
     9061 agatcaggcg cgacagaatc tggcgacaca acagacgctg gccgcatggt gcgatcacat
     9121 gtgtgaaacc tccattcagg aggagatcaa tcaggaactc atcaaatggt ctgcggggtt
     9181 tctggatcag gggcacgctc cctggccgat gcccatgcgt gaacaaacgt tttatggcgg
     9241 ttggaagatc ctggctcagc aggacggcgc cgggttcttg cagggcatcc cggattggaa
     9301 acgcaagctt caacagctgc cggaccggtc ggaagatgcc gtgttggttt gcctccaggc
     9361 cttgggtctt cctcagagtg tctggagcaa cgtgtttacc ctgcatttgg cccagctgcc
     9421 gggatggacc ggatttctga agtggcgtgc cgaccaggtt gaccatgaat ggcagaatgc
     9481 ctttcctatc gacctggttc aatatctggc ggttcgcctc ttttacgagc gtgagttggt
     9541 gcaaagagta tgtcaccatc gattcgggat tcccggcacc tatccggcca tcgtgtcctc
     9601 ccttgaggat ttttcattcg gattcgggct ctaccacgcg tttcgtaccg gaaggctcaa
     9661 tctggaaata cttcccgccc tggacgaggc tttgttcacc acaattcctc ttcctttgca
     9721 aacacttgat gcattgggac gggaagcgga cgccaaatgg ggaactgtcc aacaagattt
     9781 aacggccaag ggacacacgt tgatgctttg tcatctggct ggctccctgg gacttgctct
     9841 gaaagatg
//
LOCUS       DATIAL010000131         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1205550, whole genome shotgun sequence.
ACCESSION   DATIAL010000131 DATIAL010000000
VERSION     DATIAL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205550"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VLA60_04160"
     CDS             <1..282
                     /locus_tag="VLA60_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08582.1"
                     /translation="EISRAVARIQAGVLAFVCAVLGGLGLFIATVWLLLDGGPQVGSH
                     LQLLSNYYIGYSVTWRGSVIGMLYGALTGAILGWTIGFIYNKVVGLRGA"
     gene            344..1321
                     /locus_tag="VLA60_04165"
     CDS             344..1321
                     /locus_tag="VLA60_04165"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF08583.1"
                     /translation="MRVLITGAAGFVGNYLAESLLRDGHAVRGFVRPTKDTSVLEQLG
                     VEIARGDLADSASVERAIQGCDQVYHLAALTSRQKPTRKEAMAVNCEGTRHVVRAALK
                     AGISRMVYSSTAGVYGVISDAPVNEASPVNPDSPYQEAKVAGERLVLAAYRDHRFPVV
                     IARFPGVLGKGSMSWVPLFRAIGTGRFRIIGSGHNHTHTGHILDIVDGLRRCAETPHI
                     EGQCYLLAGKTATRIDEFVKLFAKELGVSISSMRLPNFPYRVFHRLSCLLYRSFGITL
                     PRANDYELFISDKVLSLEKAKRELGYEPKIQIEQGIRETLQWYREQSLI"
     gene            1352..2566
                     /locus_tag="VLA60_04170"
     CDS             1352..2566
                     /locus_tag="VLA60_04170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSF08584.1"
                     /translation="MRILLLNDYGVPMGGAEVLSFALRDELRKRGHDARLMTTTAGEL
                     DAKDCEEKHEADYRCFGTTTRYRTLLQTVNVWAYRALQQVLREFRPDVVHVRMFLTQL
                     SPLILPLLKSVPSLCHVVWYRPVCPVGTKLLPNGHPCRVSPGIACYQNRCLPLRDWIP
                     LMVQMSLWHQWKGAFARIIANSEATKAALVAEGIGPVEVIWNGVPVNKSKAALAKDPT
                     VVFAGRLTREKGVDVLLRAFGSLRHKVPNAKLMIAGSGHEQAALKTLVQDLGLEEAVS
                     MPGRLNRNDLENLSKTAWVQAVPSRWAEPFGLVAAEAMMQGRAVVASATGGLQEIIEH
                     GRTGFLVPPDDTEALAEKLSVLLLDQRLAETMGNAARSVARIRLSLARQVDEFVGVYE
                     QLVAGHPTPAVL"
BASE COUNT          597 a          594 c          779 g          644 t
ORIGIN      
        1 gagattagtc gggccgtggc caggatacag gcgggagtgt tggcttttgt ctgcgcggtg
       61 cttgggggat taggcttgtt tattgcgacc gtgtggctgc ttctcgatgg gggaccacag
      121 gtcggctccc atcttcagtt actttcgaat tattacatcg gatattcggt cacatggcgc
      181 ggcagtgtta tcggaatgtt gtacggggcc ctaaccgggg ctattctggg ttggaccatc
      241 gggtttattt ataacaaggt ggtggggctt cggggagcgt aaggccactt caaatattcc
      301 atagcagccg gagaatgaga cacccacgaa ggtggaaaga tcaatgagag tgctgatcac
      361 cggagcagcg ggttttgtcg ggaattatct tgccgagagt ttgctacgag acgggcatgc
      421 cgtacggggg tttgttcgtc cgacaaaaga cacctcggtt ttggaacaac taggagtgga
      481 aatcgccagg ggcgatcttg ccgactccgc ctcggttgaa cgggccatcc aggggtgcga
      541 ccaggtttat catttagctg ctctaacctc cagacaaaag cctacccgaa aagaagcgat
      601 ggcagtaaat tgcgagggaa ccaggcatgt cgtgcgtgca gctttaaaag cgggaatctc
      661 cagaatggtg tactccagca ctgcaggggt gtatggagtg atttccgacg cacccgtgaa
      721 tgaggccagt ccggtaaatc ccgactctcc ctatcaggag gcaaaagtgg cgggagagcg
      781 actggttttg gcggcatata gggaccaccg gtttcccgtg gtcattgccc gatttcccgg
      841 ggtcctggga aaaggatcga tgagctgggt gccgttattt cgggcaatcg gcaccggaag
      901 gtttcgcatc atcggatccg gtcataatca tacccatacc ggccatatat tagatatcgt
      961 ggacggactc cgtcgttgtg ccgagacacc ccatattgaa ggacaatgtt atttgttggc
     1021 gggaaaaact gcaaccagaa tcgatgaatt tgtgaagcta tttgccaagg aactgggggt
     1081 gtccatatct tcgatgcgcc ttcccaattt tccgtatcgg gtatttcata gattgagttg
     1141 tttgctgtac cgatcgtttg gaataacgct tccgcgtgcc aatgattatg aattgttcat
     1201 ctcggataaa gttctgtcac tcgaaaaagc caaacgggaa ttgggatacg aaccaaaaat
     1261 tcagatcgaa cagggtatcc gcgagacatt acagtggtat cgggaacaga gcctcatttg
     1321 aaaagtgttt tatctccgag gtcgagtccg gatgcgtatt ttgttgctga atgattatgg
     1381 cgtgcccatg gggggggccg aggtactttc attcgctctt cgcgacgaat tacgcaagcg
     1441 aggacatgat gcgcgattga tgacgacaac tgctggagaa ttggatgcga aagactgcga
     1501 ggagaaacac gaagccgatt atcgatgttt tggtactacg acgcgatatc gcactttgct
     1561 tcaaacggtc aatgtgtggg cctatcgtgc gctccaacag gttttgagag aatttcgacc
     1621 tgatgtcgtg catgtccgga tgtttctaac tcaattgtct cccctgattc tgccgttact
     1681 caaatccgta ccgagcctct gtcacgtcgt ctggtaccga ccggtatgtc cggtaggaac
     1741 caagttgtta cccaatggcc atccgtgtcg ggtgtcgccg ggcatcgcat gttaccagaa
     1801 ccgatgctta ccgttacgag actggattcc tctgatggtt cagatgagtc tttggcacca
     1861 atggaagggg gcgtttgccc gtattattgc caacagtgaa gccaccaagg ccgccttggt
     1921 ggccgaggga atcggacctg tggaagttat ttggaatggt gtcccagtga acaaatccaa
     1981 ggctgccctt gccaaagacc ccacagtggt tttcgctggt cgtctgacca gggaaaaggg
     2041 cgtcgatgta ctgcttcgtg cgtttggttc gttgcgtcac aaggttccaa acgccaaatt
     2101 gatgatcgcg gggagcggtc atgagcaggc ggcattgaag acgctcgttc aagacctcgg
     2161 ccttgaggaa gctgtctcga tgcccggtcg tctgaatcgg aacgatttgg agaacctgtc
     2221 gaaaacggcc tgggtgcagg cggtgccttc caggtgggcc gaaccatttg gactcgtggc
     2281 ggctgaggcc atgatgcagg ggagagccgt tgtggcgagt gctaccggag gactacagga
     2341 aatcattgag catggacgca caggtttttt ggtcccaccg gatgacactg aagctttggc
     2401 ggaaaagttg tcggtcctat tactggatca gaggctggca gaaacgatgg gaaatgcagc
     2461 acggagtgtg gcccggattc gattgagttt ggctcgtcaa gttgatgaat ttgttggggt
     2521 ctatgaacaa ctggttgccg gacacccgac gcctgcagtt ttgtaaagtt ccttctttac
     2581 ccagggagac caaatggatc aaggtctgaa taaa
//
LOCUS       DATIAL010000132         8536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_345944, whole genome shotgun sequence.
ACCESSION   DATIAL010000132 DATIAL010000000
VERSION     DATIAL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345944"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /gene="infB"
                     /locus_tag="VLA60_04175"
     CDS             <1..2113
                     /gene="infB"
                     /locus_tag="VLA60_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HSF08585.1"
                     /translation="PAVSPTESGGLSGVVAPPPVLEEKSVATPPSSKSPEKEKKGEGK
                     PDKKAPVLSREVPIEEKGKKIKKVARVKDEDLFAARFEDAAVWQDLRPLPTLRREERT
                     RQVQQPSVGEVTKPRKKVMKVTAGISVKDFAEVIGQKPTEIIRKLMDLNIAKTLNQPM
                     DLEAGVLIAEGYGLAVEAVAAKGGEALLDEVLEGGEQGNLEPRAPVVTVMGHVDHGKT
                     SLLDAIRKTQVTDQEAGGITQHIGASFVKAGERGVTFLDTPGHEAFTAMRARGAQVTD
                     IVVLVVAADDGPMPQTIEAINHAQAANVPIIVAVNKMDKPGANPDRVKQSLAEHGLQP
                     EAWGGQTIFVEVSAKQNKGLDQLLDMLLLQAEIMELKGDSTCSARGVVLEAKLDKGRG
                     PVATVLIQAGTLRIGDSFVVGSVSGRVRGLTSDKGERLKEAGPSIPIEVIGLLGVPEA
                     GDQLIVVKDERAAREIAQARQQKQKDVGLASTSRRTLEELYAESKDGEIKELPLLIKA
                     DVQGSVGALGDALLKISTDAVKLKIIHSGVGGINETDVLLAAASKAIIIGFHVRPDSK
                     AAAIAEREGVEIRLYTIIYNILDDIRSAAEGLLAPTIKERVMGHAEVRQLFSVPKMGT
                     IAGCYVNDGLISRSNTKVRVVRDQVMVYEGRIGSLRRFKDDAREVQQGYECGIGVENF
                     NDLKIGDVLEAYVLEEEATKL"
     gene            2139..2426
                     /locus_tag="VLA60_04180"
     CDS             2139..2426
                     /locus_tag="VLA60_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HSF08586.1"
                     /translation="MGMIVGLCTLELHIPDVQSLKGKRQVLSSLIARLRNRFNVSVAE
                     IDEQDLWQKAILGVVCVANDTGRVNQVLDQVLNFIRSNPSLEILRSQIEVL"
     gene            2449..2868
                     /gene="rbfA"
                     /locus_tag="VLA60_04185"
     CDS             2449..2868
                     /gene="rbfA"
                     /locus_tag="VLA60_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HSF08587.1"
                     /translation="MAKINSSRATRVADQIRMEVAEILSRKTKDPRIQFVTVTGVKMT
                     ADLKIARIYVTALDQAHFDQVTGPGLKSAVGFVRTELGRRLNLRYTPELVFYRDTSAE
                     YGNRIEKLLDSLHEESGDSGETEASSSLPNKSGSVEG"
     gene            2868..3857
                     /gene="truB"
                     /locus_tag="VLA60_04190"
     CDS             2868..3857
                     /gene="truB"
                     /locus_tag="VLA60_04190"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HSF08588.1"
                     /translation="MAPVASCIDHSNIGKAAQGMALHGVLNVNKPDGWTSHDVVQRLR
                     SGLGIQKVGHAGTLDPHATGVLPVLIGKGTKIAQYLLGWEKEYGAVLQLGQRTDTQDA
                     WGKVLEESPVESLTEDRIRSVFATFQGSIQQVPPMYSAVKIGGQPLYKKARRGETVDR
                     PAKTVVIHDLEIQRLNVPEVEFRVVCSKGTYVRTLCEDIGNILQVGGHLKWLQRRRVG
                     PIHVDQALEIESLLGGLEFSHLHGAFLGLDQALEGFPAVEVDRDDARKVLHGNAISWD
                     RVVDSYLEPSVSPMGDNMVRVKQRDGQLLALGRGPVFQAGKGVPVLAIETVFA"
     gene            3955..4224
                     /gene="rpsO"
                     /locus_tag="VLA60_04195"
     CDS             3955..4224
                     /gene="rpsO"
                     /locus_tag="VLA60_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HSF08589.1"
                     /translation="MGLTKEAKTAAIESHRIHDKDTGSSEVQISLLTNRITSLTEHFK
                     THKKDHHSRRGLLRMVSKRRKLLDYLRRRDEGKYQAVIAALGIRK"
     gene            4351..6456
                     /gene="pnp"
                     /locus_tag="VLA60_04200"
     CDS             4351..6456
                     /gene="pnp"
                     /locus_tag="VLA60_04200"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HSF08590.1"
                     /translation="MIHFVEMEVGGRPLRLETGRMAKQADGAVLATYADTVVLATAVA
                     SKTLKADADFLPLTVNYQEKAYSAGKIPGGFFKREGAPSEKEVLTSRLIDRPIRPLMP
                     EGYFYETQIIVSVLSIDQTMSSDVIGIVAASAALAVSDIPGSGLLAGVRIGRVNGQFV
                     VNPDKKAQEESELNLVVAGTKDAVMMVEAGADGLSEDTMIDAIELAHREIKNIVAKIE
                     ELQAKAGKPKRVLQVEAIDPELEQAVRRVVAAPICEAMYIAAKAERQDRFAQILQEAI
                     ETVAGEDADRKAKVKKVFADLEYSEVRRMILEKKKRADGRGLSDIRPISSEAGILPRT
                     HGSALFTRGETQALAVVTLGTSDDEQRIDALEGEYFRSFMLHYNFPPFSVGEAKMLRG
                     PGRREVGHGKLAERALAAVLPTKEEFPYTIRLVSDILESNGSSSMATICGGALAMMDA
                     GVPIKTPVAGIAMGLILEQGGVAILSDILGMEDHLGDMDFKVAGTRDGVTALQMDIKI
                     AGITPDLMRQALAQAREGRLHILGKMDECLAVPRTGLAAYAPRITTIQIKQDKIRDVI
                     GPGGKMIRSIIADCGVKVNVDDSGVVTIAAVDGESAQKAMDMVQRIVEEAEVGKIYLG
                     TVRKIVDFGAFVEILPGMDGLVHISQLAPHRVASVTDEISEGEQILVKVLEIDRQGKI
                     RLSRKDAMAEQTDKDQLTS"
     gene            6475..7743
                     /locus_tag="VLA60_04205"
     CDS             6475..7743
                     /locus_tag="VLA60_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSF08591.1"
                     /translation="MYKKVILDNGVRVVLERMSSLKSVALGVWATVGSRDEGKGEGGL
                     SHFIEHMMFKGTPKRTASQISNEIDALGGEMNAFTTHEATAFYVKVLDQQMRLAFDLI
                     ADLFHHSRFSAKDIAKEKQIVLEEIRTVQDDPEDYIHELHAKDIFGSHPLGRPILGEP
                     GVMKRLSRQALMQYRQQHYRPEHTIVAVAGNFSFSEIIDTVNTYFGKWKQGGYGKRSS
                     TLQKAPWPDQPQERQSLHVKPLEQVHVCVGFKGLPVGHPDRYAAHVLSTILGGGVSSR
                     LFQEIREKRGLAYTIYSHLSGFLDGGTLTVYAATRPNEMAAVIDRICQETKKLCRREV
                     ASNELERTKTQLKGSLMLGLEGTYGRMNKLAKDEMYQGRHVTLQEMVKAIDRITPEQI
                     RHLSQKLFDLQQFVVTALGPIPKRGIPRWG"
     gene            7933..8472
                     /locus_tag="VLA60_04210"
     CDS             7933..8472
                     /locus_tag="VLA60_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08592.1"
                     /translation="MKKSSLRQELVALCAFGLLSLGLVSVGYAAGPGPDKSKGDPNDI
                     THQGDISFKGVPATSLKGRVFGHWADNPEGEILFGIQYWNTSAPAETGNPPGRASDDR
                     IQTLPDSLFSCCAWGYQGGNEKNNPYSGWYTPQTTVRLAVKDKALMDQITKAAQDFVA
                     MEVQVDGRTVTGFKLLQEE"
BASE COUNT         2260 a         1763 c         2458 g         2055 t
ORIGIN      
        1 ccccgccgta tcgcccactg aatcgggagg gctttcgggt gttgtggctc ctccccctgt
       61 tcttgaggag aaaagtgtcg caacccctcc ctctagtaaa tctcctgaaa aagagaagaa
      121 aggcgaaggg aagcccgaca aaaaggctcc agttctatcg cgagaagttc ccatagagga
      181 aaaaggcaag aaaattaaaa aagttgcgcg tgtgaaagac gaagatcttt ttgccgcacg
      241 gtttgaagat gctgcggtct ggcaagatct tcgcccatta ccaacgttgc gacgtgagga
      301 acgtacccgt caggttcaac agccttcggt gggtgaagtc accaaacccc ggaaaaaagt
      361 tatgaaggtt actgcaggga tatccgtgaa agattttgcc gaagtgatcg gccaaaaacc
      421 cacggaaatt attcggaaat tgatggattt aaatattgca aaaaccctca atcaacccat
      481 ggatttagaa gctggagtgc tcattgcgga ggggtatggc ctcgcagttg aagcggtggc
      541 tgcaaaggga ggggaagccc tcttggatga ggtgcttgag ggaggagagc aaggaaattt
      601 ggaacctcga gcgcctgtgg tgacggttat gggacatgtc gatcatggaa aaacctcgtt
      661 gttggatgcg attcggaaga cccaggtgac cgaccaagag gccggaggga ttactcaaca
      721 catcggggcg tcatttgtga aagctggtga acgtggtgtg acgttcttgg ataccccagg
      781 ccatgaagcc tttacggcca tgcgggctcg tggggctcag gttactgata ttgtggtctt
      841 ggttgtggct gcagacgatg ggccgatgcc tcagacaatt gaagctatta atcatgcgca
      901 agcggccaat gtgccgatta ttgtggccgt gaataaaatg gacaaaccgg gggctaatcc
      961 tgaccgggtt aagcaaagtt tagccgagca tggtttgcaa cctgaagcat ggggaggcca
     1021 aaccattttt gttgaagtat cggcaaagca aaacaagggg ttagaccagt tgcttgatat
     1081 gttgttgctt caagcagaaa ttatggaatt gaagggggat tctacctgtt ccgctcgagg
     1141 ggttgtcttg gaagcgaaat tggataaggg gcgtgggcct gttgccacgg tgttaattca
     1201 agccggaacc ttacgaatcg gggattcgtt tgtcgtgggg tcagtgagcg gccgggtgcg
     1261 aggattaaca tcggacaaag gtgaacgctt gaaggaagcc gggccatcga taccgattga
     1321 agtcatcggc ttattgggtg tgcctgaggc cggggatcag ctgatcgtgg tgaaggatga
     1381 gcgggctgca agagaaattg cccaggcccg gcagcaaaag caaaaagatg taggcctcgc
     1441 atccacttcc agaagaaccc ttgaggagct ttatgcagaa tctaaagatg gagaaattaa
     1501 ggaattgcca ctactgatca aggcagatgt gcaaggatcg gtgggggcgt taggcgatgc
     1561 acttctgaag atttctactg acgcggtaaa actgaagatc attcatagtg gagtcggggg
     1621 catcaacgaa actgatgtat tgttagcggc tgcttcaaaa gcgattatca ttggatttca
     1681 tgttcgtccg gattcgaagg cggctgccat tgcggaacgt gagggtgtgg aaattcggtt
     1741 gtacaccatc atttataata ttcttgatga tattcgttcg gctgcagagg gactcctggc
     1801 tcccacgatc aaggagcgag tgatggggca tgcggaggtt cggcaactat tctccgttcc
     1861 gaaaatgggc accattgccg gctgttatgt gaatgatgga ttgatatccc gttcgaatac
     1921 caaagtgaga gttgttcgtg atcaggtgat ggtctacgaa ggaagaattg gatcattaag
     1981 gcggtttaag gacgatgcac gggaagttca gcagggctat gaatgcggaa ttggagttga
     2041 gaattttaat gatttaaaaa tcggcgatgt gttagaggcc tatgtattgg aagaggaggc
     2101 cactaaatta taatgctgca tggataaggg aatcctgaat ggggatgatc gtggggttgt
     2161 gcacgctcga actccatatc cctgatgtcc aatcgttgaa agggaagcgt caggtcctct
     2221 ccagcttgat tgcccggttg cgaaatcggt ttaatgtttc cgttgctgaa attgatgaac
     2281 aagacctttg gcaaaaagcc attttggggg tggtgtgtgt ggcgaatgac acgggaaggg
     2341 tcaatcaagt cttagaccaa gtcctcaact ttatacgatc aaatccctcc ctcgagattt
     2401 tacgatccca gattgaagtc ttgtaaactc ccattatttt ttcacgtcat ggcaaaaatt
     2461 aatagtagtc gggcaacacg ggttgccgat cagatccgga tggaagtggc agagattctt
     2521 tctaggaaaa cgaaagatcc acgtatccaa ttcgtcaccg tcacaggtgt gaagatgacg
     2581 gctgatctca aaattgctcg gatatatgtg accgccttgg atcaagccca ttttgatcag
     2641 gtgacgggtc cgggtttaaa aagcgcagta gggtttgttc gaaccgaatt gggacgacgg
     2701 ttaaatctgc gttatacacc ggagttagtc ttttatcgtg atacgagtgc agagtatggg
     2761 aatcgaattg aaaagttatt ggattccctt cacgaggaat ctggagattc cggggaaacc
     2821 gaggcatcct cctctctccc caataagagt ggaagtgtag aggggtaatg gcaccagtgg
     2881 cgtcttgcat cgaccactct aacatcggga aggcagcgca ggggatggcc ttacatggag
     2941 ttctgaacgt caataagcct gatggatgga cgtcgcatga tgtggtgcaa cgtctcaggt
     3001 cgggtttggg tattcagaaa gtcggacacg ctgggacctt ggatccccat gcgacaggtg
     3061 tattgccggt cttaattgga aaaggaacca aaattgccca gtatctattg ggatgggaaa
     3121 aagaatatgg cgccgtgctt caattgggcc aacggaccga tacgcaagat gcttggggta
     3181 aggtattaga agaatctcca gtggaatctc tgacggagga ccgtatccgg tcagtgtttg
     3241 cgacttttca agggtctatc cagcaagtgc cacccatgta ctcggcggtg aaaatcggtg
     3301 ggcaaccctt gtataaaaaa gccaggagag gggaaacggt tgaccgaccg gcaaaaaccg
     3361 tggtgatcca tgatttggaa atccaaagac tgaatgtccc tgaggtcgaa tttcgggttg
     3421 tctgttccaa gggaacctat gttcgaacgc tctgcgagga tattggaaac atattgcagg
     3481 tgggtggaca tttgaaatgg cttcaacgac gacgggtcgg gcctattcat gttgatcagg
     3541 cccttgagat agaatccctg cttgggggac ttgaattctc ccatctgcat ggggcatttt
     3601 tgggtttgga ccaggcttta gaagggtttc ccgcagttga ggtcgataga gatgatgcac
     3661 gaaaggtgct ccatgggaat gcgatctcct gggatcgggt ggtcgactcg tatttggaac
     3721 catcagtttc acccatgggg gacaatatgg tgagggtgaa acagagagat gggcaattac
     3781 ttgctttggg gagaggcccg gtctttcagg caggcaaggg agttcccgta ttagcaattg
     3841 aaacggtgtt tgcctagggt ataaaaaatt ggttttaatt agtgggataa gacgacttcg
     3901 tagaaggaat ttcttactga attcatgtaa ccctgaggaa ttggaggagg agttatgggc
     3961 ttaacgaaag aagcaaaaac cgcggcaata gaatcgcatc ggattcacga caaggacacg
     4021 ggttcgtctg aagtgcagat ttccttgcta acaaatagaa ttacgagttt gactgaacat
     4081 ttcaagacac acaaaaaaga ccatcattcc cgtcgaggcc ttttgcgaat ggtgagcaag
     4141 cgaagaaagt tattagatta tttgcggcgt cgagatgagg gaaaatacca agccgtgatt
     4201 gctgcactgg gcatacgaaa ataaaacaga atacaggttt tagggtgagc agaatcaatg
     4261 agatgggaga cagatgcccc gttctcaatg cgaagcgaag cctcacactc catatcaata
     4321 tatccacaca aggatcagaa aggttgaaac atgattcatt tcgttgaaat ggaagttggt
     4381 ggtcgcccgc ttcgattgga aactggtcga atggccaagc aagcggatgg tgccgtgttg
     4441 gcgacctatg ctgataccgt ggtgttagcc acggcggtag cctcaaagac cttgaaggct
     4501 gacgccgatt tcttgcctct gaccgtgaac tatcaagaga aagcctactc agctgggaaa
     4561 atacctggcg ggtttttcaa acgggaaggg gcccccagcg aaaaagaggt gttgaccagc
     4621 cgactcattg accgtccgat tcggccatta atgcccgaag gttactttta tgaaacccag
     4681 atcattgtat cggtgctctc tattgatcag accatgtctt cggacgtcat tggcattgtg
     4741 gccgcgtcgg cagcactcgc cgtttctgat attcccgggt ccgggttatt agcaggggtg
     4801 cgaattggaa gagtgaacgg ccaatttgtg gtcaatcccg ataagaaggc acaggaggaa
     4861 agcgagttaa atctcgtggt ggccggaacg aaggatgccg tgatgatggt cgaagctggt
     4921 gcggatgggc tttcggaaga cacgatgatt gacgcgatcg aactggctca tcgtgaaatt
     4981 aagaatatcg tcgccaaaat tgaagaatta caagcaaagg cgggcaaacc caagcgggta
     5041 ttgcaggtgg aagccatcga ccctgaactt gagcaagccg ttcgccgggt ggtggccgct
     5101 cccatttgtg aggccatgta tattgcagcc aaagccgaac gccaggatcg gtttgcccaa
     5161 attctccaag aagcgattga gaccgtggcc ggagaagatg ccgaccggaa agccaaggtt
     5221 aaaaaagtgt ttgcagattt ggaatattcc gaggttcgaa gaatgatcct ggaaaaaaag
     5281 aagagagccg atggccgggg tttatccgac attcgtccca tttcttctga ggcagggatt
     5341 cttccccgga cgcatggctc tgccttattt accagagggg aaacccaggc attggctgtg
     5401 gtgaccttgg gcacttcaga tgatgagcaa cggattgatg cgttggaagg agagtatttc
     5461 cggtcattta tgttgcatta caatttcccg ccttttagcg tgggtgaggc gaaaatgctt
     5521 cgtggtccgg gacggcgtga agtcggccat ggcaaattag ccgaacgggc gttagcagca
     5581 gtgcttccca ccaaggaaga gttcccttat accattcgac ttgtctcgga tattttggaa
     5641 tcaaacgggt cctcttcaat ggcgacgatt tgtggtgggg cattagccat gatggatgcg
     5701 ggagtaccca ttaaaacgcc ggtggcagga atcgcgatgg ggttaatttt ggaacagggt
     5761 ggcgtcgcga ttctttcaga tattttagga atggaagatc atctgggcga tatggatttc
     5821 aaagtggcgg gaacacgaga tggagtcaca gccctgcaga tggatattaa gattgccgga
     5881 attacccccg acctgatgcg gcaggcgttg gcgcaggccc gtgaagggcg attgcacatc
     5941 cttgggaaaa tggatgaatg cttggctgtc cctcgtaccg ggctggcagc ctatgcgcca
     6001 cgtatcacca ccatccagat caaacaagat aaaatccggg atgtcattgg gcctgggggc
     6061 aaaatgattc gtagcatcat tgccgattgt ggagtgaaag tgaatgtcga tgactcagga
     6121 gtggtcacga ttgcagccgt agacggggag tcggcccaaa aggcaatgga tatggttcag
     6181 cggatcgttg aagaagcgga ggttgggaaa atctatctcg ggacggtgcg aaagattgta
     6241 gattttggcg cctttgtgga gattctcccc ggcatggatg ggctggtgca catttcgcaa
     6301 ttggcccctc accgcgtggc atcggtcacg gacgaaattt ctgaaggtga acagattctt
     6361 gtgaaagttc tggaaattga tcgacaaggg aagatccgtc tgagccgaaa agacgccatg
     6421 gctgaacaga cggacaagga tcagctcacc tcctaagcct gcctgagtcg caacgtgtat
     6481 aaaaaagtca tattggataa tggagtccgg gttgtcctgg agcggatgtc atctttgaaa
     6541 tccgtggcct taggggtatg ggccaccgtg ggaagtcgtg acgaaggaaa gggggagggt
     6601 ggcctctctc atttcattga acatatgatg ttcaagggca cgcccaaacg gactgcatcc
     6661 caaatttcca atgagatcga tgccctgggt ggtgaaatga atgcctttac cactcatgag
     6721 gccacggctt tttatgtcaa ggtgctggat cagcagatga ggctggcttt tgatctcatc
     6781 gcggatctgt ttcaccattc aaggttttcg gccaaagaca ttgcaaaaga aaaacaaatc
     6841 gtccttgagg agatccgtac cgtccaggat gacccagagg actatattca tgaattgcat
     6901 gccaaggata tcttcggctc ccacccgttg gggcgtccga ttctgggaga gcctggggtg
     6961 atgaaaagac tcagccgaca agcgctgatg cagtataggc aacagcatta tcgccctgaa
     7021 cacacgattg ttgctgtggc cggcaacttt tccttttcag aaatcattga tacggtcaac
     7081 acatattttg gcaagtggaa acagggtgga tacggcaagc gttcctcaac cctccagaaa
     7141 gcaccctggc ccgatcagcc gcaggaacgc caaagtcttc acgttaaacc cttggagcag
     7201 gttcatgtgt gtgttgggtt taagggattg ccggtggggc atcctgatcg gtatgcggcg
     7261 catgtcttga gcacaattct gggaggcgga gtcagttcgc gattattcca ggaaatccgg
     7321 gaaaaacggg gattggccta taccatttat tcgcatcttt caggtttttt ggatggtggg
     7381 acgctgaccg tttatgccgc cacccgcccg aatgaaatgg cggccgtcat tgaccgaatt
     7441 tgtcaagaaa ccaagaaatt atgtcgtcgg gaagtggcga gcaatgaatt agagcgaacc
     7501 aaaactcaat taaaaggaag tctgatgttg ggcttggaag gcacatacgg gcggatgaat
     7561 aaattggcaa aagacgaaat gtaccaaggg cgtcatgtca ccctccagga gatggtgaag
     7621 gccattgatc ggattacccc tgaacaaatt cgtcatttaa gccagaaatt atttgatcta
     7681 cagcagtttg tggtcacggc attgggaccc atcccaaaac gaggaattcc gaggtgggga
     7741 tgatcatcaa aaccgcttat tttttcgctt gacaaccatg ccctggatga gtagcattgg
     7801 accctctcga atacctggtt ttcctttttg gattaggggt atgcagaact tgggaaaagt
     7861 ggtttagaag ataaacaaag ggtaagtcgt tttttaattt tctgtcattc taacactgta
     7921 aggagggagc agatgaagaa gtcatctcta cgtcaggaat tggtagctct ctgtgccttt
     7981 ggcctgttga gtcttggatt agtaagcgtt gggtatgcag ctggtcctgg tcctgacaag
     8041 tcaaaaggtg acccaaatga tatcactcac cagggtgata tttcttttaa aggcgtacca
     8101 gctaccagtt taaaggggcg ggtttttggc cattgggcgg acaaccctga gggagaaatt
     8161 ttattcggaa tccagtattg gaacacctct gcaccggcag aaacaggcaa tcctccaggc
     8221 agggcttccg atgatcgaat tcaaacgctt cctgactcat tgttttcctg ttgtgcctgg
     8281 gggtatcagg gcgggaacga aaagaacaat ccatattcag gatggtatac cccgcaaaca
     8341 acagtccgtt tagcggtcaa ggacaaagcg ttaatggatc aaatcacgaa ggcggcccaa
     8401 gatttcgtgg ccatggaagt gcaagtagat ggccgtacgg tcacgggatt taaattgtta
     8461 caagaagaat aagaaattgt ctgataattc atgattagaa ttaaaatttt agtcgaggag
     8521 ggaagccatt atgaaa
//
LOCUS       DATIAL010000133         4675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_560602, whole genome shotgun sequence.
ACCESSION   DATIAL010000133 DATIAL010000000
VERSION     DATIAL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560602"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..372)
                     /locus_tag="VLA60_04215"
     CDS             complement(61..372)
                     /locus_tag="VLA60_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HSF08593.1"
                     /translation="MVLLEFSMSPLGKGESVGKYVSRSLDLIDKSGVEYRLNPMGTVL
                     EGEWDDVFRVVKQCYTRMKKDCPRISCVIKVDYRRGHKGRLAGKVASVEKHLKRKLKT
                     A"
     gene            complement(372..1028)
                     /locus_tag="VLA60_04220"
     CDS             complement(372..1028)
                     /locus_tag="VLA60_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HSF08594.1"
                     /translation="MTKNAGFQVTVDLDGRQCMVIGGDEEAVEKVHRLLDAGAKITVV
                     NPTLHVDLRKLTASGKIIHRGRTFRSGDAQGVVLIMNVLKDDLDLAKSLFELAKTERF
                     LVWSMDLPEYSTIMMPALVKRGNLRIAISTSGTAPALAKVLRHNLELLFDEEFDQFLD
                     WLGALREELKSTEMSDLRRRERLAEAVNGFQLSGELQYPNSWKLSSEEQVEPVGKEGG
                     "
     gene            complement(1242..2141)
                     /gene="metF"
                     /locus_tag="VLA60_04225"
     CDS             complement(1242..2141)
                     /gene="metF"
                     /locus_tag="VLA60_04225"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HSF08595.1"
                     /translation="MHISEVLRTYNPAISFEFFPPKTERGFQELFEAISALMPLKPAY
                     VSVTYGAGGSTRERTHDLVVKLQRETDLTIVSHLTCVGSRRDEIHHILSKYQESGIHN
                     IMALRGDPPKGSTDVQIPEDGFQFASDLVRFIKQQFPEMGVGVAGFPEGHPGTPNRLQ
                     EMDNLKRKVDAGADYICTQLFFDNRDFFDFCERCEVVGIKVPIIAGIMPIVSRKGMAR
                     MSELALGARIPAKLLRAMDRAENDAYAEGVGIHWATEQVVDLVDHKVKGIHFYTLNKS
                     KATLKIYESLGIQSSESLQQPVE"
     gene            complement(2174..3262)
                     /locus_tag="VLA60_04230"
     CDS             complement(2174..3262)
                     /locus_tag="VLA60_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08596.1"
                     /translation="MVDGFLLFTVLDVWRMVLPLGLTLLLLMTDLLCRWQSDSPHKGL
                     RDVLSPLVLRLTLGLLVLSGIVGLLVPAILGYPLPESHIAGGPWSYEEILGFVLGGIC
                     LWKSRPQECPGGNSGLVWLACASMFFFGAGVVWIGRWDAMAHEGYVGGGWFLLGLSTV
                     DWTRVIPKSLHLLFASLAAGGLVVTLLGLLGWSGASNGSASTVLSRFLPSDERVRYGV
                     GWMLTGLVPQVLIGPWLFLVLGEVPRGGLIDGAGLPSVFFFVGVTTALLAMVLLNASF
                     MVPQVKGLVWGGLICTVITLVLMGMIRYVMFLGTLQAQGIPIAIGNLTLFHLLTVLVL
                     TGLVGAILVRWCVWPIAVLQSGPNRAGD"
     gene            complement(3413..4231)
                     /locus_tag="VLA60_04235"
     CDS             complement(3413..4231)
                     /locus_tag="VLA60_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF08597.1"
                     /translation="MSDDMLSSDTSAFIQIQHATVYRGTQPVLYDLSLVIAQGGSTVI
                     LGPNGSGKSTFMKLLSMELYPVLGPDRYVRLLGKEHWNVWDLRSQIGLISAEMQQAYP
                     CHTTGLDIILSGFFSSLEVYEHQTISLRHRTRSSEMLSMLGIAHLRDRPFGEMSTGEQ
                     RRALLGRALVHEPQILVLDEPTTGLDVQACFQYLQIIRNFILQGGTVILVTHHVHEIP
                     PEIERVVLLKQGRIMKDGPKGDVLNDQSLSALFETPVRLFHDQGWFQIVPALPG"
BASE COUNT         1352 a         1394 c          956 g          973 t
ORIGIN      
        1 cctcaacaca ccgatgcctt caccacacgg ccaccctagc tcgttcaaac cgtttcagga
       61 tcatgccgtt ttcagcttac gcttcaaatg tttctccacg ctcgccacct taccggccaa
      121 ccggcctttg tggccacgcc ggtaatcaac cttaatcaca caagaaatcc tcggacaatc
      181 ctttttcatc ctggtatagc attgcttcac gactcgaaac acgtcatccc actccccttc
      241 caacaccgtt cccatgggat tgaggcgata ctccaccccg cttttatcaa tcaggtccag
      301 tgaccgggac acatattttc cgacactttc acccttacca agaggtgaca tgctaaattc
      361 caacagcacc attacccacc ttcctttcca acgggttcca cctgctcttc actggacaac
      421 ttccaactat tgggatactg caattccccg gacaattgaa agccattgac tgcttccgcc
      481 aagcgttccc gacgccgcag atcgctcatt tccgtgctct ttagctcctc gcgtagggcc
      541 ccgagccaat ccaaaaactg gtcgaattct tcatcaaaca gcaattcaag attgtgacga
      601 agcaccttgg ccagagccgg agcggtccca cttgtactaa tcgctattcg aagattcccg
      661 cgtttgacca gcgcaggcat catgatcgtg gagtattcag gcagatccat cgaccacacc
      721 aggaagcgtt cggttttcgc cagctcaaat aaggacttgg ccaagtccaa atcatccttc
      781 aacacattca taatcaacac aaccccttgg gcatcccccg atcgaaatgt ccgtccccgg
      841 tggataattt ttcctgaggc ggtcaacttc cgcaaatcaa catgaagggt gggattgacc
      901 acggtaattt ttgctcccgc atccaacaag cgatgcacct tctccacggc ttcctcatca
      961 ccaccaatca ccatacactg gcggccatcc aaatcaaccg ttacttgaaa ccctgcattc
     1021 ttcgtcatga gtatcctcct ttagaaccca ggttatctta cccaagatac accacaaggg
     1081 gaaaggggaa aatccccaat cccctggccc acccaattac ataactcacc gccaccacaa
     1141 aacacaggaa gagggaaaca gtggaccagg ccacaagagc tgagaaaccg aatgcccatc
     1201 attgatgaat ggagaaaaat tcaggattac tgaggcagga cttattcaac cggttgctgc
     1261 aaactttccg aactttgaat accgagagac tcatagattt tcaaggtcgc ttttgattta
     1321 ttcaaggtat aaaaatgaat cccctttact ttatgatcca caagatccac cacctgttcg
     1381 gtcgcccaat gaatgccaac cccttctgca taagcatcat tctcagctcg atccatagcc
     1441 cgtaacagtt tggcaggaat acgggcgcca agcgccaact cagacatgcg tgccatgccc
     1501 tttcgggaaa ctatcggcat aatgccggca ataataggca ctttaatgcc cacaacttcg
     1561 caacgttcac aaaaatcgaa aaagtcccga ttatcaaaaa ataattgggt gcagatataa
     1621 tcagctcccg catcgacttt tcgcttcaaa ttgtccattt cctgtaaccg gttgggcgta
     1681 ccgggatgcc cctcgggaaa tcctgccaca cccacaccca tttcaggaaa ctgctgttta
     1741 ataaaccgca caaggtcaga cgcgaactga aagccgtctt ccggaatctg gacatccgtg
     1801 cttcccttag gaggatctcc cctcaaagcc ataatgttat gaataccgct ctcctgatat
     1861 ttcgagagaa tatgatgaat ttcgtcacgc ctggacccca cgcaggttaa atgcgacaca
     1921 atggtcaaat cggtttcccg ttggagcttt accactaaat catgcgtgcg ttcacgggta
     1981 gagccgccgg caccataggt cacactgaca taggcaggct ttaacggcat gagggccgaa
     2041 atcgcttcaa acagttcttg aaacccccgt tccgtttttg gagggaaaaa ttcaaaacta
     2101 atggcaggat tatacgtccg caacacttct gaaatatgca tgtccatacc ttaaattgct
     2161 caccaagatt tgtctagtct ccagcacggt tcggccctga ttgaagaacc gcaatgggcc
     2221 atacgcacca gcgaacgagg atcgctccaa caagacctgt cagaaccaga acggtcagga
     2281 gatgaaacag tgtcagattt ccaatcgcaa tgggaattcc ctgtgcctgc agggtgccca
     2341 gaaacatcac ataccggatc atccccatca gcaccagtgt gatcaccgta caaatgagtc
     2401 ctccccaaac aagccctttg acctgcggca ccataaatga cgcattcaac aagaccatgg
     2461 ccagcaacgc cgtcgttacc cccacaaaga agaagacaga gggcaaaccc gccccatcaa
     2521 tcagacctcc cctcggcacc tcacccaaga cgagaaacaa ccagggtccg ataagcactt
     2581 gcgggaccag ccccgttaac atccatccaa ccccgtaacg aacgcgctca tcagaaggaa
     2641 gaaagcggga tagaacggtt gaggcggaac cattcgacgc accggaccat ccgagcagac
     2701 caagcaacgt caccaccaaa cctcctgcag ccaagctggc aaaaaggagg tgcaggcttt
     2761 tcggaatgac ccgagtccaa tccacggtcg acaatccaag gagaaaccac ccaccgccca
     2821 catacccctc atgagccatc gcatcccatc gcccaatcca tacgacacct gctccaaaaa
     2881 agaacatgct tgcacaagcc agccagacaa ggcctgaatt tcctccagga cattcctgcg
     2941 gccggctttt ccaaagacag attcccccca acacaaaacc cagtatttct tcatagctcc
     3001 acggtccccc ggcaatatgc gattccggca gcggatatcc aaggatggcg ggcacaagaa
     3061 gaccgacaat cccggacagc accaataaac cgagcgttaa gcgaaggacc aagggactga
     3121 gaacgtcgcg caggccttta tggggggaat cgctctgcca tcggcacagt aagtccgtca
     3181 tcagcaacag aagcgtcaat cctaacggca ataccatccg ccatacatcc aagaccgtaa
     3241 agaggagaaa cccgtcaacc atatccgttt tctttcctta acatatgatt taaaaaaatg
     3301 gaaaacttca ggtccaggcc atgccggatg aacaaaaagc ctttagggaa tagaacacgt
     3361 cccggaaggc aaagtcatcc tcagcccatt aaaaggtctt catgacccga ttttatccgg
     3421 gtaaggccgg aacaatctga aaccatccct gatcatgaaa aagacgaacc ggagtctcaa
     3481 aaagggcaga caagctctga tcattcagaa catccccctt aggaccatct ttcatgatcc
     3541 ggccctgttt caacaacaca acccgctcaa tctccggagg aatttcatgg acatggtgcg
     3601 tcaccaaaat caccgtgcct ccttggagta tgaaattccg aatgatctga aggtattgaa
     3661 aacatgcctg cacatccagc ccggtggttg gctcgtccaa cacaagaatc tgaggctcat
     3721 gaaccaaagc ccgacccaac aaagctcgcc gttgttctcc tgtcgacatc tccccgaaag
     3781 ggcgatcccg aagatgggca atcccgagca tgctcaacat ctcagaactt ctggtgcgat
     3841 gtcggagact aatggtttga tgttcataca cctccaaact agagaaaaac cccgaaagaa
     3901 taatatccag cccggttgta tgacagggat acgcctgttg catttctgcc gaaattagcc
     3961 cgatctgtga acgtaggtcc cacacattcc agtgctcctt ccccaacagc cggacatagc
     4021 gatccggccc gaggaccgga taaagttcca tcgatagcag cttcataaat gtcgatttgc
     4081 ctgaaccatt tggacccagg atgaccgtcg aaccgccctg agcaatcacc agagacagat
     4141 catagaggac aggctgagtg ccccggtaca ccgtcgcatg ttgaatctga atgaaagcgg
     4201 atgtgtctga actcaacatg tcatctgaca tccacaaagc cctcctgccc catgaacaag
     4261 taaccacggc ccacaggcca tatcaaacac ataacgtctt cccggaattg gtagagtcca
     4321 tagacctcaa gcgagggccg tggatgtccc ggcaaattct cttaccccaa aacccagcaa
     4381 ctctatcctt taccaaaaca aaccgtctca cacaaagaca aattgttcca tcctgaattc
     4441 ataaccaaac tggaaatcca cgtaatgctc acaatcaggc catcgtttat aataaacaga
     4501 tacaaccatc actggcacat cagttgcttc tgaaaaggtt gagagagaga aagaaaaccg
     4561 aacgggacgg ccaacattca ccattttcag cagacgtata aaacgaggga ggtcgcaatg
     4621 aaaaggatgt cttcaggatg cgcaatcggg actatggcct ttgtgtccgg aatcg
//
LOCUS       DATIAL010000134         5680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_668084, whole genome shotgun sequence.
ACCESSION   DATIAL010000134 DATIAL010000000
VERSION     DATIAL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668084"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VLA60_04240"
     CDS             <1..379
                     /locus_tag="VLA60_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08598.1"
                     /translation="GRHTAESTSNNITRPPCTGKNVHDKQTIKFDKADHVIQSIQVCT
                     TDKKKKDDKIKGAEIWAVRVGPDGTLFEASLSDKFRRPNCERWREKVSCPSNQIAIGI
                     EASWGDGGFAGLRLRCKAVGPKE"
     gene            926..4075
                     /locus_tag="VLA60_04245"
     CDS             926..4075
                     /locus_tag="VLA60_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08599.1"
                     /translation="MASEKSDDFKIPITGLLILLTVLGGILFYQQPFKSERPVHEQQN
                     QAVIVGDKRVQSRLWQDPFASVKAHKLAEPKANQSQTVAQSSQGSLHGSETTDHHNLR
                     MQLREITRPFGEHPPTFKALIVVTEGTPFASGTEYRLRHRYAVVSALGEAGYIPEEGE
                     FFRYFSWNRTQLQGKDTCWDESCPALDVPLEWFKPGNTGKDHVLVFWVKDQDLEKNPL
                     ASLEGLVAFVNDSVPDSLQERRVNDSVDATMSFTVLGPFHSGTLRAMISEVLGRHKMA
                     PPSNTSTAESTPIQYLSYSATASEAVLFEGLNIDKAQCFSEAECWKYMKSLGFDVHRV
                     IGTDEELATELAEELHKRNVDFTDEPDHIALIGEWDTLYGRKLPLTFLEVTHRTRDLD
                     LLTKIRNLGDVEWPDWAHRFSYLRGIDGELPQSANASGGKSEGDGTLSQNVFKINKGL
                     ERPEGPAQFDYLRRLALQIQVEDRRIREACLNKVGEGSWPTCPGFKAIGILAGDVYDK
                     LLLLQALRKEFPGVLFFTTDLDARLLHPGQYQWTRNLLIASHFGLRLDDKVQGSIPPF
                     RDAYQTSLFLSTLLAIDPDAVCHTASGLFAHGICDNSPDQPSLHRAPRVFEVGLTGAY
                     DLSDGTRSIHVSRPDFEHGEPAPPIGLRTFILLGCILLSSWLIYAAMSRKAVFLTGAA
                     VKSPMFWAFLFAVVGSYSWLFVSITNQLEHGEPFVWLEGISVWPTEILRLAAFFLSLC
                     FLCRGYRTLQAKFRQLEADFDLSPGECSGCFEWKKWMEFGPDFSRGSAPPKHTVRETW
                     RSYTSLMGLRCAGSRVVAQTAVYLILFAFPIFLLIGFPTTPCRGETCFYWDKAILILS
                     VVTMWLLVFFVVYVTRLCARMVDWVLQPGVEWPPRNTAREPWFHSSRKDALANIQRSL
                     WFCGQLTEVVGTLIYYPFIIVLVMIVSRSSFFDDWDLPISLLIVIGSNIGLAIFSAFH
                     FRQTAENARGRVLNDLTSMLSTNSSSLGKARVQKVISEVQNFKKGAFCPFAEQPILKA
                     FAIPSGGYFLMLFIEYLGR"
     gene            complement(4155..>5680)
                     /locus_tag="VLA60_04250"
     CDS             complement(4155..>5680)
                     /locus_tag="VLA60_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF08600.1"
                     /translation="HGVRIKDSALVAASRLSNRYIGDRFLPDKAIDLIDEASARLRTE
                     IDSMPAEMDEISRKVMQLEIEREALKKETDAASRTRLTHIEEELKAKQAALNDLKTQW
                     DAEKSSVGKLQTIRQEIEGVKQQIEKAERQYDLNRVAELRYGTLPKLEKSMRDEEERL
                     ANQQGTSRLLKEEVDEDDIAEVVSRWTGIPVSRLLQGEVEKLLHLDEWLHKRVIGQDE
                     AVKAVAEAVLRARSGIKDPNRPIGSFLFLGPTGVGKTELARALAHTLFDNEANMVRID
                     MSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRRPFSVILFDEIEKAHHDVFNVLL
                     QVLDDGRLTDSQGRTVDFKNTVLIMTSNLGSQDILEAQQRNMDYEGIRALVLKEIQQH
                     FRPEFLNRVDEIVVFHPLKREELAQIVEIQLERLRVRLADRRMSLELSPAAVADLAAR
                     GYDQVYGARPLKRLLQQEIETPLSRLIIQGKAMDGQRIVGDLVDGKFVLTARENVEA"
BASE COUNT         1230 a         1496 c         1496 g         1458 t
ORIGIN      
        1 tggtcgccat acggcagaaa gcacatcgaa caatatcacc cggccgcctt gcacggggaa
       61 aaatgttcac gacaagcaga cgattaaatt cgacaaggcg gatcatgtta tccagtccat
      121 ccaggtctgc accaccgaca agaaaaagaa agacgacaag attaaagggg cggagatctg
      181 ggccgtgcgt gtgggcccgg atggaactct cttcgaagcc agcctcagtg acaagttcag
      241 acgcccgaac tgtgaacgct ggcgcgagaa ggtttcctgc ccgagcaacc aaattgccat
      301 tggcatcgag gcctcctggg gtgatggcgg ttttgccggc ttgcgccttc gttgtaaagc
      361 cgtcgggccg aaggaatgag cagcgggtat gcgtcggtca agcctgcgcc ttaccgtggt
      421 cgagaatgga tcgacgtgtc tgcggatctg ttcgacgata aaatcccata cacacgcttc
      481 tgtaatctgc acaaacagga taggttcccc tggcgtatgg tggacagatc gccaaatttc
      541 tccctggaaa aaagacggga gtccgttcct tcaacgaatg acaggaccac gtgtctgatt
      601 ggatacaggg tcgtatctga ttggatacgc aatcgaccac tcaacaccta gaccgttttc
      661 catcggcgtt ccttttgttt cattcttttt ctttcattac gcgggtttgg cattcgttcc
      721 ctcagtggcg agagggtcat ccgctgcgct cgcatggatc ttgagtttcc tgtaggtgag
      781 gcacggcacc ggatccggca gtatggatca ctggacgttt tgaatggttc acccaaatat
      841 atttctttcc gcatccgcaa tgagatggaa aagaaaagcc tcatccggca gttgcccggt
      901 tccagggaac gctcgcgagt gacttatggc ctctgaaaaa agcgacgatt ttaaaattcc
      961 tattacgggg ttattaattc tgctcaccgt gctgggtgga attttgtttt accaacaacc
     1021 ctttaaaagt gagcggcccg ttcatgaaca acagaaccag gcggtgatcg tgggtgacaa
     1081 acgagtgcaa agccgtttgt ggcaggaccc gttcgcatcg gtgaaagctc ataaactggc
     1141 cgaacccaaa gccaaccagt ctcagactgt ggctcagtca tcccaaggtt ctctccatgg
     1201 ttcagagacc accgaccacc ataatttacg catgcagtta cgtgagatca ccaggccttt
     1261 cggggaacac ccccccacat tcaaagccct gattgtcgtg acagaaggca ctccctttgc
     1321 ttccggaacc gaataccggt tacgacatcg ctatgcggtg gtgtccgctc tgggagaggc
     1381 ggggtatatt cccgaggaag gggagttttt ccgatatttt tcgtggaacc ggacacagct
     1441 tcaaggaaaa gacacctgct gggatgaaag ttgtcccgcc cttgatgtgc cgcttgaatg
     1501 gtttaaaccc ggcaacaccg gaaaagacca cgtgttggtc ttctgggtca aggatcaaga
     1561 cctggagaaa aacccgttgg cctccctgga aggcctcgtg gcctttgtga acgattcggt
     1621 acccgactct ctacaggaac gaagggtgaa cgattctgtt gatgccacga tgtcgtttac
     1681 ggtgttaggc ccgtttcatt ccggaacctt acgcgccatg atcagtgaag ttctgggaag
     1741 acataaaatg gcgcctccct caaatacctc tacagctgaa tcaacaccca ttcagtatct
     1801 ttcctattcg gccaccgcct ctgaggcggt tctttttgaa ggccttaata ttgataaggc
     1861 acagtgtttc agcgaagctg agtgctggaa gtatatgaag tccttgggtt ttgatgtgca
     1921 tcgggtcata gggacagacg aagaactggc cacggaactg gcagaggaat tacacaaacg
     1981 aaatgtggac tttaccgacg aaccagatca cattgccttg atcggggaat gggatacgct
     2041 ctacgggcgg aaactgcctc tgacctttct tgaagtgact catcgtactc gggatcttga
     2101 tctgttaacc aagatcagga atttgggaga cgtggaatgg cccgattggg cccatcgctt
     2161 tagctacctt cgtggcattg acggtgagct tcctcagagt gcgaatgcct ctggaggaaa
     2221 atccgagggt gacggaaccc tttcccagaa cgtttttaaa attaataagg ggcttgaacg
     2281 gccggaaggc ccggcccaat tcgattatct gagacggttg gcgttgcaaa tacaagttga
     2341 agatcgaagg attcgagaag cgtgtctgaa caaggtggga gaaggatcat ggccaacctg
     2401 tcccggcttt aaggccattg gcattttggc tggagatgtc tacgacaaac tgctcctcct
     2461 ccaggccctg cgaaaggaat ttcccggagt ccttttcttt accaccgatc ttgatgcccg
     2521 cctcttacat ccgggccaat accaatggac tcgcaacctt ctcattgcct cccatttcgg
     2581 ccttcgtctt gatgacaagg tgcaagggtc gattcccccg tttcgggatg cttatcaaac
     2641 ctcattgttc ttgtccacgt tgctggccat tgaccccgac gccgtgtgtc acacggcttc
     2701 aggattgttt gctcatggga tatgcgacaa ctcaccagac caaccctccc ttcatcgagc
     2761 accaagagtc ttcgaggtcg gactcacggg tgcgtacgac ctgagtgacg gcacccgctc
     2821 catccatgtt tcacggccgg actttgaaca tggagagccg gcgcccccca taggtttacg
     2881 gacattcatc ctgctgggat gtattctgtt gagtagttgg ctgatttatg cggccatgag
     2941 ccggaaagcg gtgtttctta ccggagcggc ggtcaagtct cccatgttct gggccttctt
     3001 gtttgccgtc gtcggtagct attcctggct gtttgtgtcc atcaccaacc aactcgagca
     3061 tggagaaccc tttgtctggc tggaaggtat cagtgtctgg ccaacggaaa tactcagatt
     3121 ggcggcgttc tttttgagtc tgtgctttct ttgccgggga tacagaaccc tgcaggccaa
     3181 atttcgtcaa cttgaggcag atttcgatct ctctccagga gaatgttccg gatgtttcga
     3241 atggaaaaag tggatggaat ttggtcctga tttttcaagg ggcagcgcac cccccaaaca
     3301 tacggtgcgg gaaacatggc gcagctatac gtcattaatg ggtctacggt gcgcaggatc
     3361 ccgtgttgtc gcccagacgg ctgtctactt gattctgttt gccttcccta tctttttgct
     3421 catcggattt cccaccaccc cctgccgggg tgagacgtgt ttctattggg ataaggcgat
     3481 tcttattctg agcgtggtca ccatgtggct cctggtgttt tttgtggtct atgtcacacg
     3541 gctttgtgcc cggatggtgg attgggtgtt acaaccaggc gttgaatggc caccccgaaa
     3601 tacggcacga gaaccatggt tccatagttc acggaaggat gctctggcca acattcaacg
     3661 cagtctgtgg ttttgtggtc agctcacaga agtggttggg accttaatct attatccctt
     3721 tatcattgtc ctggtcatga tagtatcacg ctcttcattt tttgatgact gggatcttcc
     3781 gatcagtctt cttattgtga tagggagcaa catcgggctg gccatttttt ccgctttcca
     3841 tttccggcaa acggctgaaa acgcacgagg acgcgttctc aatgacctga cgtccatgtt
     3901 gagcacgaat tcaagctctc taggaaaggc tcgggtgcag aaagtcatca gcgaggtgca
     3961 gaactttaag aaaggggcgt tttgcccgtt tgccgaacag cccatcttga aggcctttgc
     4021 tattccttcc ggtggctatt tcctcatgct gttcattgaa tatcttggca gatgatatga
     4081 cggaaatccg ttgaggaggg caatccaacg gggagcacca agatccttct ccccgttgga
     4141 ttgatgcagt ggcgttaggc ctctacgttt tcccgggcgg tgaggacgaa tttgccgtcg
     4201 accagatcgc ccacaatgcg ttggccatcc atggccttgc cctggatgat cagccgggag
     4261 agcggcgttt cgatctcctg ttgaaggagg cgtttcaagg gacgggctcc atagacctgg
     4321 tcgtagcctc gcgcagccag atcggccaca gccgccggcg aaagctccag ggacatccgc
     4381 cggtccgcca atctgaccct gagccgttcc aactgaattt cgacgatttg cgccagctcc
     4441 tcccgtttta acggatggaa gaccacaatt tcatcgaccc ggttcaagaa ttctggacgg
     4501 aaatgctgtt ggatttcttt gagcaccaag gcgcgaatgc cttcgtagtc catattccgt
     4561 tgctgggctt ccaaaatatc ctgactgccc agattcgagg tcatgatgag caccgtgttt
     4621 ttgaagtcca ccgttcggcc ttgtgagtcg gtcaaccgcc cgtcatctaa gacttggagc
     4681 aggacattaa agacatcatg atgcgccttt tcgatttcat cgaagaggat gaccgagaag
     4741 ggtctccgcc tcaccgcttc ggttaactgc ccgccttctt catagcccac ataccctggc
     4801 ggggcgccga tcaaccgggc cacggtatgt ttttccatat attccgacat atcgatacgc
     4861 accatgttgg cttcgttatc gaagagggta tgcgccagcg cccgcgccaa ctcggttttg
     4921 cccacgccgg tcggtcccag gaagaggaac gacccgatgg ggcgattggg atctttgatg
     4981 ccggaccggg cacgcaacac ggcctcggcc accgctttga ccgcttcatc ctgaccgatc
     5041 acgcgtttgt gtaaccattc atcgaggtgc agcagttttt ctacttcgcc ttggagtaac
     5101 cggctcacgg gaatgcctgt ccaccggctg accacctcgg caatgtcatc ttcatcgacc
     5161 tcttctttga gcagacggct ggtgccttgc tgattggcca gccgctcttc ctcgtctctc
     5221 atggattttt ccagtttggg caaggtgcca tagcgtaatt ccgccacgcg attcaaatcg
     5281 tactgccgct cggccttttc gatttgttgt ttgacgcctt cgatttcctg ccggatcgtt
     5341 tgtaattttc cgacagacga tttctccgca tcccattggg ttttcaagtc attcagcgct
     5401 gcttgtttgg ctttcaactc ttcctcaata tgggttaacc gggtgcggct ggcggcatcg
     5461 gtttctttct ttaaggcttc ccgttcaatt tccaactgca tgacttttcg ggagatctca
     5521 tccatttcgg caggcatgct gtcgatttcg gttcgcaatc gcgcgctggc ttcgtcgatg
     5581 aggtcaatcg ctttatcggg tagaaaccgg tcaccgatgt agcgattgga caggcgtgaa
     5641 gctgccacta atgctgagtc tttgatgcgc acgccatgat
//
LOCUS       DATIAL010000135         4359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_239031, whole genome shotgun sequence.
ACCESSION   DATIAL010000135 DATIAL010000000
VERSION     DATIAL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239031"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VLA60_04255"
     CDS             <1..670
                     /locus_tag="VLA60_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF08601.1"
                     /translation="EHLSLTVEPGEHVALVGPSGVGKSTLMKLLFRYYDVGEGAIELD
                     GYDIRHLPLFYLREQIGFVQQEPFLFNGSVRANLAYGDLSASHDQIEAVARAAQAHEF
                     IQALPDGYDTWIGERGVKLSVGQKQRIAIARVLLKNPPIVVMDEATSNIDTETEVEIR
                     IAMDELMRGRTTFIVAHRLSTLQTVDRIVVLEHGQIVEEGDHATLLARGGLYAGLYEA
                     QFHV"
     gene            1013..1204
                     /gene="yacG"
                     /locus_tag="VLA60_04260"
     CDS             1013..1204
                     /gene="yacG"
                     /locus_tag="VLA60_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HSF08602.1"
                     /translation="MTVKCPMCGNVEQWEGNVFRPFCSKRCQLLDLEGWLSERYRIPD
                     AEEDGLDETNSETSPTSSD"
     gene            1226..1765
                     /locus_tag="VLA60_04265"
     CDS             1226..1765
                     /locus_tag="VLA60_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08603.1"
                     /translation="MPKMITAKRHEDDTEKAQEFVRTNILLPASLLGLISVVVGVIAL
                     GFQFWAGTYGWETFTYSSALIIAGVLVGIVQTKYQQYLLREFPGHFASRMKAYSQRSV
                     RKAKKAITEHDIEHRGRGLVPLWYFLGIVMFFALSGLAVATGFMQPVAAFALPWAGYF
                     WAKLFFWKGVVLLPERKKR"
     gene            complement(1805..2914)
                     /gene="lpxD"
                     /locus_tag="VLA60_04270"
     CDS             complement(1805..2914)
                     /gene="lpxD"
                     /locus_tag="VLA60_04270"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HSF08604.1"
                     /translation="MTISSSSRTVNIPLQELAQAIHATIHGSSDILISGLSHLEGASS
                     GDVSFVLKPSFQKVARQSQAAAFIVTEPIPEDPRPQLMVPNPLVAVTTLAQKFFLPPL
                     PPRGIHPSAVSGLDVRIGPDVSIGALVTIGDRVLIGSGVTIHAGVHVGDDAIIGDECI
                     LYPHVSLLSKCVIGNRVIVHSGTVIGSDGFGYVQHEGRHHKIPQLGHVIIEDDVELGA
                     NVTVDRATFGSTVIKRGTKIDNQVQIAHNVVIGEDCILVAQVGIAGSTTLGRHVMVGG
                     QAGLVDHVTIGDQVKIAAGSGVTNDVKSGQIVGGRPAVEHGIWRRSQVLQYQLPELRK
                     ELRALQKQVRHLESLLTDQSASKSPPVKRRSTSKP"
     gene            complement(2911..3282)
                     /locus_tag="VLA60_04275"
     CDS             complement(2911..3282)
                     /locus_tag="VLA60_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSF08605.1"
                     /translation="MKSSVNKSSQLSQQVYHTLGKYLQRNPKDLHPEDSLRDDLGLDS
                     LQTIELVYEVESAFDLQIPDEDFGRLTTIGAVILYLDERIHGKESTLSGPQATPLAKN
                     SRPTPFVKKPKSRPTSKKSRT"
     gene            complement(3279..>4359)
                     /locus_tag="VLA60_04280"
     CDS             complement(3279..>4359)
                     /locus_tag="VLA60_04280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HSF08606.1"
                     /translation="FRLPDPSEDKFSSRVDRYAQFALAATREAIQDSRLDLEKEPAER
                     IGVMAGVGMGGMMMGERELTTLYQSRKPHRVHPNFIPTITLNSASGILALAFGAKGPN
                     LTISTACSSSIHSIGQAMQAIRLNQADVVIASGADASITPLVFAGFCSLRALSTKYND
                     HPDQSSRPFDRGRDGFVMGEGAGTLILESLRHATRRKAKIYAELAGYAATSEAYHMVI
                     PREDGSDIARTVTLALKDAGVSPSQVDYVNAHATSTVVGDDVEVKGLRAVFGKRLNSI
                     MVNATKSLIGHTLGAAGAIGTIVSALSIQTGIIHPTVNYDDPDPHCALPGLSTKVQKK
                     SVRVGLVNAFGFGSNNAVLVLKKFS"
BASE COUNT         1009 a         1050 c         1253 g         1047 t
ORIGIN      
        1 tgaacatctt tcactgacgg tagagccggg ggagcatgtg gctttggtcg ggccgagcgg
       61 ggtgggaaaa agtacgctca tgaaactgct cttccggtat tacgatgtcg gggagggcgc
      121 gattgaactg gatggatacg atattcgtca tctcccatta ttctaccttc gagaacaaat
      181 cggctttgtg cagcaagagc catttttatt caatggcagc gtgcgggcca atctggccta
      241 tggagacttg tcggcatcac acgatcaaat agaagctgtg gcgcgcgctg cgcaagcgca
      301 tgaatttatt caagcgcttc ctgacgggta tgacacctgg atcggggaac gcggggtgaa
      361 attgtccgtc gggcaaaaac agcgtatcgc cattgccagg gtgctgctca aaaatccacc
      421 cattgtggtc atggatgaag ccacctccaa tattgacacc gaaaccgaag tcgagattcg
      481 cattgcgatg gatgagttga tgcgggggcg gacgaccttt atcgtggccc atcggttgtc
      541 gactctgcaa accgtggatc ggattgtggt cctggaacat ggacagatcg tggaagaagg
      601 cgatcatgcg accttactgg cgagaggggg attgtacgcc ggtttgtatg aggcccaatt
      661 tcatgtctaa catgacaccg cacacatcta tacttgtcca ggcttgtggt ctatcgacct
      721 atgagtaggc atgaacgaag gggccgggtg tatcggaagg actgttgatt atttttcgaa
      781 ttttttctct tcatgactag atgagtgaca gaactccaca cgggaaatca tgagggtaaa
      841 aaaagcttgt ccagccctgc cctaagttct ctcgaaggga agtccgcccc tgctttgggc
      901 ggggagagcg tacggaggaa gacgtgagca cggccaaagg gcgagaaggc atcgttataa
      961 gtccctccaa gggtgcaaca cgtgtttccc acactccctc gggaggaacg atatgaccgt
     1021 gaaatgtccc atgtgtggca acgtggaaca atgggaagga aatgtcttcc gacccttctg
     1081 ttccaagcga tgtcaattgt tggatttgga gggatggttg agtgagcgct atcgcattcc
     1141 ggatgcggaa gaagatgggt tggatgagac gaattctgag acaagcccaa catcttccga
     1201 ctagaccgta tatggagatc aatgtatgcc aaaaatgatc acggctaaac gtcatgagga
     1261 cgacacggaa aaggctcagg agtttgtccg gaccaatatc ctgcttcctg ccagtttgtt
     1321 ggggttgatt tcggtcgtcg tcggagtcat cgccttagga tttcagttct gggcaggaac
     1381 gtatggatgg gaaaccttta cctacagctc ggcactcatc atcgccggcg tgcttgtggg
     1441 aatcgttcaa acaaaatacc aacagtatct tttgcgggag tttcccggac attttgccag
     1501 tcgcatgaaa gcctatagcc aacggtcggt ccgtaaggca aaaaaggcca tcacggagca
     1561 tgacattgag catcgaggtc gtggattggt tccgctctgg tactttctgg ggatcgtgat
     1621 gtttttcgct ctttccggat tagccgtggc cacgggtttt atgcagccgg tcgccgcgtt
     1681 tgccctgccc tgggcaggct atttttgggc aaagttattt ttttggaaag gggtggtgtt
     1741 gctgccggaa cgcaaaaaga gatgaagccc tacggctgct gggtttcgga tccgctctgg
     1801 tggattaggg ctttgaggtt gaacgacgtt tgacaggtgg ggacttggat gcagattggt
     1861 ccgtgagcag cgattcaaga tgtcggacct gtttttgaag agcccggagt tccttgcgta
     1921 attcggggag ttggtattga agaacctgtg accgtcgcca gatgccatgt tcgaccgccg
     1981 gccgaccacc cacaatttga ccggatttca catcattggt cacccccgag ccggccgcaa
     2041 ttttcacttg atcaccgatg gtgacatgat cgaccaggcc ggcctggcca cccaccatga
     2101 cgtggcggcc taatgtggta cttcccgcaa ttcctacctg tgcgacgaga atacagtctt
     2161 ccccgatcac cacgttatgg gcgatctgca cctggttatc gatttttgta ccgcgcttaa
     2221 taacggtgct cccaaatgtt gcccgatcca ccgttacatt ggccccgagc tccacatcat
     2281 cttcaatgat cacatggccc aattggggga ttttgtggtg acggccttcg tgttgcacat
     2341 agccgaaccc atcgctcccg ataacggtgc cgctgtgtac aatgacgcga ttgccgatga
     2401 cacacttgct cagcagcgag acatggggat acaggatgca ctcgtccccg ataatagcat
     2461 catcccccac atgaaccccg gcatggatgg tgaccccgga tccgatgagc acgcggtctc
     2521 caattgtgac gagggcgcca attgagacat ccggaccaat gcgcacatcg agtccggaga
     2581 cagcactggg gtgaatgcca cgtggtggaa gaggggggag gaaaaatttt tgggccaacg
     2641 tcgtgacggc gaccagcgga ttcgggacca ttaattgtgg tcgaggatcc tcgggaatag
     2701 gctctgtcac aatgaaggct gcagcctgtg attgacgcgc cactttttga aacgaaggct
     2761 tcaggacaaa ggagacatct ccggaggagg ctccttcaag atgggagagc ccggatatca
     2821 gaatgtcaga ggatccgtga atggtggcgt gaatggcttg ggccaattcc tgaagaggaa
     2881 tattcacggt tcgtgaggaa gacgatatcg tcatgtgcgc gatttttttg aagtgggacg
     2941 gcttttgggt tttttgacaa acggagtcgg acgtgaattc ttggccaacg gtgtggcttg
     3001 agggccggag agggtgctct ctttaccatg tattcgttca tccagataaa ggatgacggc
     3061 gccgatggtt gttaatcgcc cgaagtcctc atccggtatt tgaagatcaa aagccgattc
     3121 gacttcatag actaattcga tggtttgcag agagtccaac cctaaatcat cacggagcga
     3181 atcttccgga tgcagatctt tcggattgcg ttgaaggtat ttaccgaggg tgtggtacac
     3241 ctgttgactg agttgagagg atttgttgac ggaagatttc atgaaaactt ttttaagacc
     3301 aggaccgcat tgttacttcc gaatccgaag gcattcacca agcccacccg aacagatttt
     3361 ttttgtacct tagtagaaag tcccggcaag gcacaatggg gatccgggtc atcatagttc
     3421 acggtggggt gtattatacc agtttgaatg gataaggccg agacaattgt cccgatcgct
     3481 ccggccgctc ccagtgtatg gccaatgagc gatttggtgg cattaaccat gatggaattt
     3541 aggcgttttc cgaacaccgc ccgtaaccct ttgacctcga catcgtctcc gaccacggtg
     3601 gatgtcgcat gggcattcac atagtcaacc tgggagggcg aaacccccgc gtctttcaat
     3661 gccagagtaa cggttcgcgc aatatcggag ccatcctctc tgggaatcac catatgatac
     3721 gcttcgctgg tggcggcata tccggccagt tccgcatata ttttcgcttt tcgacgggtc
     3781 gcatggcgaa gtgattccaa gatcagggtg ccggcccctt cgcccatcac aaatccgtcc
     3841 cgccctcgat cgaatggcct ggaggactga tcgggatggt cattatattt ggtggataag
     3901 gcgcgtaatg agcaaaagcc ggcaaatacc agcggggtaa tgctggcgtc cgctcctgag
     3961 gcaatgacga catctgcctg attcaggcga atggcctgca tggcttgtcc aattgaatga
     4021 atgctggagg agcaggccgt agaaatggtg agatttgggc cttttgcccc aaaagccaag
     4081 gccagaatgc ccgaggcgga gttgagcgtt atggtcggaa taaagttcgg gtggacccga
     4141 tgtggcttac gagactgata gagggtggtg agttcgcgtt cccccatcat catgccgccc
     4201 atgcccacac cggccatgac tcctatccgt tcagccggtt ccttctcaag gtccagtcta
     4261 ctatcctgga tggcttcccg tgtggcggcg agagcaaatt gtgcataacg gtccacgcgc
     4321 gatgaaaatt tatcttctga aggatcggga aggcgaaag
//
LOCUS       DATIAL010000136        15443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1098750, whole genome shotgun sequence.
ACCESSION   DATIAL010000136 DATIAL010000000
VERSION     DATIAL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15443
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098750"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VLA60_04285"
     CDS             complement(<1..624)
                     /locus_tag="VLA60_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HSF08607.1"
                     /translation="MANPLSKKELDLIDAYWRAANYLSVGQIYLYDNPLLKKTLTKEH
                     IKPRLLGHWGTTPGLNFLYVHLNRLIKKHDLNMIYITGPGHGGPGLVANAYLEGTYSE
                     VYPNISQDEEGLKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAAYDNP
                     DLIVACVVGDGEAETGPLATSWHSNKFLNPATDGVVLPILHLNGYKIA"
     gene            complement(717..1970)
                     /locus_tag="VLA60_04290"
     CDS             complement(717..1970)
                     /locus_tag="VLA60_04290"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HSF08608.1"
                     /translation="MRMLVLNSGSSSVKFRVFEYGREGSSHEVCLNRTLIKGAVKGIG
                     STASFEYEIEDYPGTKTTRLISDHQQAIHWMFEQLERFEREGHHQCSLAGVEAVGHRV
                     VHGGERFSQPVVITEEVLGEIDVLSELAPLHNPACLEGIRGARAILGTRVPMVAVFDT
                     AFHRTMPTHAKVYALPYELADRYHIRRYGFHGIAHASLAGNYFDKTGKPSQESRLITL
                     QLGNGCSISAIKGGQSLETSMGFTPLEGLVMGSRSGDLDPSIVNYLCQKEKVDPSEVD
                     RWLNEQSGLLGLSGRTNDMRELLQAAEREQHEPSQMAIEVFCYRIKKYIGAYLAVLGG
                     ADAIVFGGGIGEAAPVIRRRICEGMEWCGLRLDHARNKAAIGLQQGDVMQISQDGQPL
                     GMYVGAADEETWIARETVRCLKATQ"
     gene            1969..2109
                     /locus_tag="VLA60_04295"
     CDS             1969..2109
                     /locus_tag="VLA60_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08609.1"
                     /translation="MISSYSERLPGHERTRRNGWLGVEIEEAKEGMAETRGKHSSGPV
                     AP"
     gene            complement(2332..3399)
                     /locus_tag="VLA60_04300"
     CDS             complement(2332..3399)
                     /locus_tag="VLA60_04300"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSF08610.1"
                     /translation="MKHGSLEATAMALVAEGKGILAADETVGTISKRFESLKISSTEE
                     SRRTYREMLFTCPGVSEFISGVIMYDETIRQKDSKGTPLAQVCTSMGIIPGIKVDLGA
                     KDLAGSLGEKVTEGLDGLRDRLSEYYRMGARFAKWRAVIRITDRLPTQACLSVNAHAL
                     GRYAALCQEQGLVPIVEPEVLMDGPHTIKRCGEITGQMLHAVFNSLYAQRVLLEGMLL
                     KPNMVISGKECAKQASVQEVAQATLQCLCRHVPAAVPGIVFLSGGQHDRLATAHLNAI
                     HQLPSPKPWKISFSYGRALQDAALEAWHGKSENFQAGQQALLHRAKCNNAASLGQYTE
                     EMEEDPARITAPPHREDWHDD"
     gene            3826..4416
                     /locus_tag="VLA60_04305"
     CDS             3826..4416
                     /locus_tag="VLA60_04305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSF08611.1"
                     /translation="MSEKPQEVYVIRHGETEWSLSGQHTGTTDLPLTENGRNLARRLK
                     PLIANHSFDLVITSPLQRARETCLLAGLGEKAEVDSNLVEWNYGEYEGLTPKEIHEKR
                     PGWMIFTDGCPGGETPEQVGTRADRAIGRVRMVEGDVILFAHGHILRVLVARWLGFQA
                     TAGQHFLLHTGTLNVLSYYRGIPALKIWNAPLFPVQ"
     gene            complement(4800..5054)
                     /locus_tag="VLA60_04310"
     CDS             complement(4800..5054)
                     /locus_tag="VLA60_04310"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HSF08612.1"
                     /translation="MGSDPFSALLQWFGSSNGGPGTGFLTLGRYAALCQEQGLVPIVE
                     PEVRMDGSHTIDRCEEMNGHALHTIFNALYAGGFYWRASF"
     gene            complement(5107..5670)
                     /locus_tag="VLA60_04315"
     CDS             complement(5107..5670)
                     /locus_tag="VLA60_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08613.1"
                     /translation="MLKNVQAMGMSFTFITVIVLALGWPTAQAMTAAEIDVDVQKVLT
                     DLYAQSDTAKDFGAVAKGILVFPDVYKAGFLVGANFGDGALLKNGRTAGYYRSLELHT
                     DCRPASNPSAMCCFFMQDSVLEYLDKSAGWEIGVGPSLVVVDKGFAGSMTTTTAKDDI
                     YAFFFDQKGLMAGLGLKGSKITRIHPD"
     gene            complement(5781..6776)
                     /locus_tag="VLA60_04320"
     CDS             complement(5781..6776)
                     /locus_tag="VLA60_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08614.1"
                     /translation="MVGVAVSICLILVDFTGIAMAVPTTEELLKALPLSDSEREDVLK
                     GEIVRFTTEEGSDRELAMGAVLLVRNVPENLVPLWREAIVFKLVGAVTAFGEISDKAP
                     VNDFAELKLQPNGEDETKRYLEAEPGDKLNLDPKEISVFQALKAKGGSQADVEALVRE
                     QLLDRYKAYREKGLSGIPPYARDGEKMFQLSQDLLQATTEAKLVAKYFPSFHQTLLKY
                     PAITAKQLESRFYWANVEVFGRPTLILSHRMSFNEGDAYVAVDRHFYASHEYNGLQSI
                     GGALPAKEGSLLVSLHRISTDGVAGFGSSAKHPVARGLMGPYFEEIFEGVREKVK"
     gene            7379..8212
                     /locus_tag="VLA60_04325"
     CDS             7379..8212
                     /locus_tag="VLA60_04325"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HSF08615.1"
                     /translation="MGLQSKIADLGRLHEIIRAFSIAGFGYIFKKMGLEAAAERTGKI
                     MGWKYAEDIAHLERPQRIRKLFEVLGPTFVKLGHILATRPDLFGPEWISEFEKLQSQA
                     PQLNFEELRPQIEEDLGAPIEEIFEEVDTKPLAAASMAQVHRATLKDGTKVILKIQRP
                     GIRAKIESDMRLLIYLASLAEKEVPELATYHPQKVVQQFVKSLQNELSFMTEGQNTEQ
                     VAANFEGNDQVVIPKIYWEWTKERISAQEFVEGIPGVNIQAIDEAGMDRKRIALRGFR
                     F"
     gene            complement(8856..9230)
                     /locus_tag="VLA60_04330"
     CDS             complement(8856..9230)
                     /locus_tag="VLA60_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08616.1"
                     /translation="MRAGQDSNHSMPLESSQIITCVLPDDGTDRILLRILREEKDIIR
                     AASVSCRGIAVLREALTKKDRLPESTLVRMVTVVVLDTEAEALFAYIYEKARIGRPGG
                     GMLLLSKPVVSTPFSIPEDLPY"
     gene            complement(9227..9595)
                     /locus_tag="VLA60_04335"
     CDS             complement(9227..9595)
                     /locus_tag="VLA60_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HSF08617.1"
                     /translation="MTYLTDVAMITCVVQSGEADVIVKAARDAGATGVLVYHGRGTGI
                     RERLGILGFAVDASKDVISVLISSERTDIVASNIFKAGQFDTPARGFLYITPLEKAAI
                     YIPEAVKERLQNKGSAKEGL"
     gene            complement(9609..11396)
                     /locus_tag="VLA60_04340"
     CDS             complement(9609..11396)
                     /locus_tag="VLA60_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HSF08618.1"
                     /translation="MSDSYRFGDYQRALGHGYDELSFNSMKPNLEWDAQGHLIPYQPL
                     RICLTAEEIYKLLRPYVSVRFMEQVKSVIPLALYLALFQILILRQLVQNSWMIMSGLF
                     AVIVGLMFFMEGLKLGLMPFGTIIGKRLPEKSSLPLVLVITLLLGIGVTFAEPAIGAL
                     KVAGQNVVVEKAAYLYVLLNEWSGTLVLVIGFSVGLAAVIGTLRFLYGWSLKPFIYAS
                     LAPVLALTLYGASDPDLINVLGLAWDAGAVTTGPVTVPLVLALGIGIATAAGKGDSGL
                     SGFGIVTMASLFPVIGVLSLAFYIASTVTPAEIIAAANHVSGAGQSVAPVWYARSPGM
                     EIVLGVRAILPLVLFLFVVLRLILKDSLPNKGEILYGIGLTIIGMCVFNLGLTYGLSQ
                     LGGNVGSLVPAAFMSLSGVPISPIYVYSVGLILSLVFAWGLGFGATVAEPALNALGTT
                     AENLTNGVFKKKTLIMAVSLGVAVGIATGLAKLIFDLPLVWLILVGYLLAFMLTIFSS
                     EAFVNVAWDSAGVTTGPITVPLVLAMGLGFGNATHAVEGFGILSMASIGPILTVLATG
                     LWADYKVKAQARAALEVRASTQVEEVQAI"
     gene            complement(11861..12238)
                     /locus_tag="VLA60_04345"
     CDS             complement(11861..12238)
                     /locus_tag="VLA60_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HSF08619.1"
                     /translation="MVTSKRLQLLCFSSSMIILAGCGELGIHDTESLLLPSFRRSEVI
                     GFVAGLGTTFAALPDLIGMLKRRSSKGMNPRMAAIMGVFQIVWVYYGLLILSRPVILW
                     NIIAVVTNFISVGAYFYFARREH"
     gene            complement(12261..12464)
                     /locus_tag="VLA60_04350"
     CDS             complement(12261..12464)
                     /locus_tag="VLA60_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08620.1"
                     /translation="MNTAFAQKPALQKWARETLTVDLVVMMNPRTGGLDGDDRVGPEL
                     QSSGFYVDLKPWAYHVFKVSIWT"
     gene            complement(12512..13987)
                     /locus_tag="VLA60_04355"
     CDS             complement(12512..13987)
                     /locus_tag="VLA60_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSF08621.1"
                     /translation="MKAFSAHPLIYQINTRVWLTDLSKSIGRPATLDDTPDTELDRLA
                     KLGFDWIWFLSVWQTGPAAQAISRANPGWRREFAETLPDLKDEDIAGSGFAIQDYTVH
                     RDLGGAEALARLGKRLHTRGLKLMLDFVPNHMAPDHPWINEHPEYFVHGSETDLTRSP
                     RNYCRVHTKNGPLVLAYGRDPYFEGWPDTLQLNYGNPETQEAMIGELLRISGQCDGVR
                     CDMAMLVLPDVFERTWGLQAELFWPKATQRVRKQFPDFCFMAEVYWDREWTLQQQGFD
                     YTYDKRLYDRLREGHARTVRDHLRADPTYQNKMARFLENHDEPRAATTFAPGVHEAAA
                     IITFLSTGMRFFHQGQFEGRRKRISPHLVRAPLEPTNDTLHRFYDRLLAVLRQRAVRD
                     GQWCLLDAVPAWDGNGTWDSFLIFAWQGPRKERLLVTVNYAATQSQCYVRLPFPDLAG
                     NKVRLKDQMGDACYERDGDELLSRGFYLDIPAWGYHVFELS"
     gene            complement(13984..>15443)
                     /locus_tag="VLA60_04360"
     CDS             complement(13984..>15443)
                     /locus_tag="VLA60_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSF08622.1"
                     /translation="WEYPWYAAWDLAFHTLPLSIVDPDFAKQQMQLMLKGVYLHPNGQ
                     MPAYEWNFSDVNPPVHAFATLFLLRTEQALSGETDLDFLRETFNKLVLNFTWWVNRKD
                     RFGKNVFEGGFLGLDNIGVFDRSAPLPTGGHLEQADGTAWMALFSQNMLELAIELAAH
                     DPIYEDMVQKFAEHFYYIAGAMNRPGRDGMWDEEDGFYYDLLRLPDGSAQRLRIRSMV
                     GLLPLCATTVIEKSQRESIPEALAQIAERIRRIPELIKTIHPTGPGHYGMAERGIMAL
                     VNPDRLRRILTKMLDENEFLSPYGIRSISKFHEQHPFVFHVQGQEYRVDYLPGESNTG
                     MFGGNSNWRGPVWMPVNVMIIRALLNFYLYYGDNFKIECPTGSGKMMNLFEVSKDIAD
                     RLSRIFLRDERGRRPVYGGTEKFQSDPHWRDHLLFYEYFHGDNGAGLGASHQTGWTGL
                     VAKTIQLYGFLDAKLVLETGKRGAFKETVGSRAKS"
BASE COUNT         3764 a         4069 c         3984 g         3626 t
ORIGIN      
        1 ggcaattttg tagccgttga ggtggagaat cggtaagact accccatcgg ttgccggatt
       61 caagaatttg ttggaatgcc agcttgtcgc aagcggtccg gtttctgcct cgccatcacc
      121 gaccacgcaa gccacgatca gatcgggatt gtcgtatgca gcgccatagg catgtgagac
      181 ggcatagcct aactcaccgc cttcatggat cgagccgggc gtttcaggag cgacatgact
      241 aggaataccg cccggaaagg aaaattgggt gaagaggcgc ttcaaccctt cttcatcctg
      301 tgagatgttc ggatacactt cactatatgt cccttctaga taagcattcg caaccaggcc
      361 aggcccacca tgtccaggtc ccgtaatgta aatcatgttc aagtcgtgct ttttgattaa
      421 ccggttcagg tgaacataga ggaaattcag tccaggggtt gtcccccagt ggccgagaag
      481 cctgggtttg atatgctcct tggtcaaggt ctttttcaat aacgggttgt cgtacagata
      541 aatttgtccg acggacagat aattcgccgc ccgccaatag gcatcgatca agtccagttc
      601 ttttttggat aagggattcg ccatttgacc tcctttataa tgacatcata aagcctgaaa
      661 aactgcagtg aataatttgg atgcggcgct ccatgttcgg agagaaccgt cgggagttac
      721 tgtgtcgctt tgagacaacg cacggtctcc cgtgcaatcc aggtttcttc atccgcagct
      781 cccacataca ttcctaatgg ttgcccatcc tgactgatct gcatgacatc tccttgttga
      841 agtccgatag ctgccttgtt gcgggcatgg tccaaccgta atccgcacca ttccatcccc
      901 tcgcaaatcc tccgccggat aacgggagcc gcttcgccga ttccacctcc gaatacaatg
      961 gcgtccgctc cgcccagcac ggcaagatag gcgccgatat attttttgat acgatagcaa
     1021 aacacctcaa tggccatttg cgatggttca tgctgttcac gttcggctgc ttgcagtaat
     1081 tccctcatat cgttcgttct tccggaaagt cccagaagcc cggactgttc attcagccag
     1141 cggtccactt ctgacgggtc cactttttct ttctggcaga gataattcac aatcgaagga
     1201 tcaaggtcgc cggatctgct tcccatcacc agcccctcta acggggtgaa tcccatagaa
     1261 gtctctaagg attgaccgcc ctttatggca gatattgaac atccattgcc gagttgcaag
     1321 gttatgaggc gggattcctg ggaaggtttt cctgttttat caaaataatt tccagcgagg
     1381 gaggcatgcg caatgccatg gaatccgtat cgacgaatgt gataccgatc ggccaactca
     1441 tagggaagcg cgtacacttt tgcatgggtg ggcatggtcc gatggaaagc ggtgtcgaac
     1501 accgccacca tcgggacccg cgtgcccagg atggccctcg ctccccgaat cccttcaaga
     1561 caggccgggt tgtgcaacgg ggctaattcg ctgagtacat caatttcgcc aagcacctcc
     1621 tcggtaatca cgaccggttg tgaaaatcgc tctcctccat ggacaacccg gtggccgacg
     1681 gcttccactc cggcaaggga acattgatga tgtccctcgc gttcgaatcg ttcgagctgc
     1741 tcaaacatcc aatggatagc ctgttgatga tccgagatca atcgggtggt tttggtgcct
     1801 gggtaatctt cgatttcata ttcaaagctg gctgtactcc cgatgccttt caccgcacct
     1861 ttaatcagtg tccggtttag gcagacttcg tgacttgacc cttcgcgccc atattcgaac
     1921 acacgaaact tcacggatga acttccgcta ttcaacacga gcattcgcat gatctcttcc
     1981 tattcagaac ggttgccagg tcacgaacgg acgcgtcgga acgggtggct cggcgtggag
     2041 atagaagagg ccaaggaagg aatggcggaa acccgcggga aacattcatc cggaccggtc
     2101 gccccataga tgcaaaactg ttccttcttg tcctccgcaa aaaaaccgtg attgttccaa
     2161 aggcgcgtcg tttcgatgaa catttccacc ctgtactcgt tcaaaaaagt cttcatcacc
     2221 ctttgcgtgg acatatttgc tgagagcgca catgtccgca tttgttgtag tattttgggg
     2281 gtcgctgcat tgttcctttc gccagacaac gggaaacttc ccaagtgcgg cttagtcatc
     2341 gtgccagtct tcgcggtgtg gcggggcagt tatacgagcc ggatcttctt ccatttcttc
     2401 cgtgtattgc ccaagactcg ccgcgttatt acatttggct ctatgcaata atgcctgctg
     2461 gccggcctga aaattttcgg acttcccatg ccaggcctcc agcgcagcgt cctgcagggc
     2521 ccggccatat gaaaagctga ttttccaggg ttttggcgaa gggagttggt gaatggcatt
     2581 caaatgtgcc gtggccaggc ggtcatgttg gccgcctgat aaaaagacaa tacccggaac
     2641 agccgcgggt acatgacggc aaaggcattg caatgtggct tgagcgacct cctgtaccga
     2701 agcctgcttg gcgcactctt tgcctgaaat aaccatattg ggtttcaaaa gcatgccctc
     2761 cagcaaaacc ctctgggcat acagggagtt gaaaacggca tggagcattt gaccggtaat
     2821 ctctccgcaa cgtttgatgg tatggggccc atccatcaga acctcaggct cgactatggg
     2881 aacgagccct tgctcctgac atagggccgc atagcgtccc agcgcgtgtg cattgaccga
     2941 caggcaagcc tgagttggta gcctgtcggt aatccgaatg acggctctcc atttggcgaa
     3001 tcgggcgccc attctgtaat actcgctcaa tctgtcacga agcccgtcca atccttccgt
     3061 cactttttcg ccgagcgatc ctgcaaggtc tttagccccc aaatccactt tgatccccgg
     3121 gatgatcccc atactggtac agacctgcgc caggggagtt cccttggaat ctttttgccg
     3181 gatcgtttcg tcatacataa tcacgccgct gataaactcc gatactcccg gacatgtaaa
     3241 aagcatttcg cggtaggtgc ggcgactttc ttccgttgag ctgatcttca gggactcgaa
     3301 tcgttttgag attgttccta ccgtttcatc ggccgcaagg atgccttttc cttcagctac
     3361 caaagccatt gccgtcgctt caagactgcc gtgtttcatg gtgtgaagtc ctcccttgcc
     3421 tttgcttgcc gaaggagcaa aagaaatgcc atttagttca aattggggcg cctatcataa
     3481 cataaagtgg actggcttca taaagggctg tttcaagttc aagtgcaacg ggtaattgtt
     3541 ggtgacgcag gtgtgtaaac acctccaggg ggtggcgacc agtcaagact ttttctcggt
     3601 tttcttcgac actcatgtgt gtgtaggata tcgtgtttct tgcaacaccg aagtccatcc
     3661 tagctaaagt gcctcacttg tagctagaac ctaatcagga aaaaatgggg aagaatggga
     3721 caaggggaat cctaagactg ttctcttttg ggacaggtat tgacagtagg gaaggacaaa
     3781 aaggtagagt tttctctttc cttgataatt tcatttggaa tactgatgtc agaaaaaccg
     3841 caagaagtct atgttatacg tcacggagag acggaatgga gccttagcgg ccaacacaca
     3901 ggtaccaccg atttgccgct taccgaaaat ggtcgaaacc tggccagacg tcttaagccc
     3961 ttgattgcga atcactcctt tgacctagtt atcaccagtc ctctgcagcg tgcacgggaa
     4021 acgtgtctgt tggcaggtct gggcgagaag gcagaggtgg actccaatct tgtcgagtgg
     4081 aattatggag agtatgaagg gcttaccccc aaggagattc acgaaaaaag acccgggtgg
     4141 atgattttta ctgatggatg cccgggtggg gaaacccctg agcaggtcgg aacgcgagcc
     4201 gaccgtgcca ttggcagagt ccgaatggtg gaaggcgatg ttatactatt tgcccatggc
     4261 catattctac gggtgctggt cgcccgatgg ctcggttttc aggcaactgc cggccaacat
     4321 tttctccttc ataccggcac cttgaatgtt ctgagttatt accgcggaat ccccgccctg
     4381 aagatttgga atgctccgct ttttcccgtc caataagggc tccgcctgct ctggtcccgt
     4441 ctcgaggttc ttctaaagtt cattacgtga ggtatcgggc ctcctgacct tcatgggacg
     4501 tttgttcaat agaccctgac caaatatggt tttgtcatca tgtcagtctg cccggcgcgg
     4561 tcgagtcgtt ttccgggaag aatttccttc catctcttct gtgtattttc cgaggctggc
     4621 ggctccgtta cctttggctc tctgagaaaa tgtctgttgg ccagttttga tattgttact
     4681 atcgccatgt caggcgcttc aacgcagcat tctgcagggt ccgctcatat gaaaagtttt
     4741 ttcacggctt cggcaaagga agggtattga tggcattcaa aaaatgacca tattgggttt
     4801 tagaaggatg ctctccagta aaatcctccg gcataaagag cgttgaagat agtgtggagc
     4861 gcatgaccgt tcatctcttc acagcggtct atagtgtggg aaccatccat ccgcacctcg
     4921 ggctcaacta tgggaacgag cccttgctcc tgacagaggg ccgcatagcg acctagtgtc
     4981 agaaatccgg ttcccggccc gccatttgac gaaccgaacc actgtaatag agcagaaaat
     5041 ggatcacttc ccatttttcc ccctaatata aaaaggaaag cgtctccttg gagaataggg
     5101 gtttccttag tcaggatgaa ttcgagtaat tttggatcct ttcaatccaa gccctgccat
     5161 gaggcccttc tgatcgaaaa agaaggcgta gatgtcgtcc ttggcagtcg tggtcgtcat
     5221 ggaacccgca aaacccttgt ccaccacgac aaggcttggg cctacgccta tctcccatcc
     5281 cgcactttta tcgaggtatt ccagaaccga atcttgcata aaaaaacaac acatagccga
     5341 aggattggac gccggcctgc aatccgtatg aagctcgagt gaacggtaat accctgcggt
     5401 ccttccgttt ttcagaagcg caccgtcgcc aaaatttgca cctaccaaaa aaccggcttt
     5461 atagacatcg ggaaagacca ggatgccctt ggcaaccgcc ccgaagtctt tggctgtgtc
     5521 gctttgcgca tagaggtctg tcaatacctt ttgcacatcc acatcaatct cagcagcggt
     5581 catcgcctgt gccgtgggcc agccgagtgc taaaacgata acggttataa aggtaaatga
     5641 cattcccatc gcttgaacat tcttcagcat gaatgcctcc tgatgaaaac atctttgaga
     5701 aagtttggat gtatggtcgg tctaaaaaat aagaacgctt ccattttcat gaagatagat
     5761 ttgtagacca ggtgctcagg ctatttcact ttttcacgaa cgccttcaaa gatttcttcg
     5821 aaatagggcc ccattaatcc acgggcgacc ggatgtttcg ccgaggatcc gaagccggcc
     5881 accccatcgg tggaaatacg gtggagggaa accaacagcg agccctcttt cgcgggaagt
     5941 gcgcccccga tcgactgcaa gccattatac tcgtggctgg cataaaagtg tcggtcaacc
     6001 gccacatacg catcgccctc attgaatgac atccgatgac tcaagatcaa tgtcggacgg
     6061 ccgaacacct caacattagc ccagtaaaat cggctttcga gttgcttagc cgtaatggct
     6121 ggatatttga gtaatgtttg atggaatgag ggaaagtatt tggcgacaag ctttgcctcg
     6181 gtggtggcct gaagaaggtc ttgactcagc tgaaacatct tctcaccatc tcgtgcgtag
     6241 ggaggaatgc ctgacaaacc cttttcacga taagctttgt accggtccag caattgttcc
     6301 cgcaccagtg cctcaacgtc agcctgagac ccgcccttgg ctttaagagc ctgaaaaacc
     6361 gaaatttctt tcggatccaa attcaactta tcccccggtt cagcttcgag gtagcgtttg
     6421 gtttcgtctt ccccattggg ttgcaatttc aactctgcaa agtcattcac tggcgcctta
     6481 tcagaaatct cgccaaatgc agtaaccgcg ccgacaagct taaatacgat agcctcccgc
     6541 cagagcggaa ccaaattttc agggacattc cgcaccagca ataccgcccc cattgccagt
     6601 tcccgatctg agccttcctc ggtcgtaaat cgcacaattt cccccttgag gacatcttcc
     6661 cgttcactat cggaaagagg caaggctttg agtaattcct cggtcgttgg aacggccatc
     6721 gcaataccgg tgaagtccac caggatcaga cagatactta ccgccacccc aaccatggag
     6781 acgaacctca aattcggaaa acatttttta gccctgagga atgatgaaga aggactcaag
     6841 ccagatgatg gcaacatagt catttcccct cccatgatac aaatagacta ttaataaatt
     6901 tatatttggc tttgggttac tagggttatg agaaagatag ccagatggga aacatatacc
     6961 cttagggagc acaaaatttt ttctggtaat atcgcaaccc tattcatgga aaaaattacc
     7021 ctctcctttt aaaactaatg aacacacaca atccgccgtg gagcgggatc agttcaatgc
     7081 ctcaagccaa aatttgattg tgagatttga aggaaggccc acaggcttgc cagggataac
     7141 gcctttagtt atttgcaatt tctttttctt cccaaacata ctatattaca gcaaatttcc
     7201 ataggggagt ccagatttga acaaagacgg aaaacttaaa gacgaacaac cagcaagcat
     7261 tcaccacggc ttttttcacg ctgggcattt ttcacaactc gtagttgggg ttcagccgaa
     7321 ctttgcagcg aatttcagtg gcacaaaaaa ggtacgggga tccaaggaag gctagtagat
     7381 ggggctccag tccaaaattg ccgacctggg gcggcttcac gaaatcatca gggcgttcag
     7441 tatcgcaggc tttggctata tcttcaaaaa gatgggactt gaagccgcag ccgaacgcac
     7501 aggcaagatt atggggtgga aatatgcaga agacattgcc cacctcgaac gtcctcagcg
     7561 tatacggaaa ctctttgaag tacttgggcc aacttttgtc aaactcggtc atattcttgc
     7621 cactcgtccg gacctgttcg gccctgagtg gatcagtgaa ttcgaaaaac tccaatctca
     7681 ggcaccacaa ctgaacttcg aagagcttcg tcctcagatc gaagaagatt tgggtgcacc
     7741 gattgaagaa atcttcgagg aggtagacac caaaccgctg gctgccgctt ccatggccca
     7801 agttcaccgg gccactttga aagacgggac caaagtgata ctcaagattc aacgcccagg
     7861 gatccgggcg aagattgaat ccgatatgcg gctgttaata tatttggcgt cattggctga
     7921 aaaggaagtc ccggaactgg ccacctatca tccccaaaaa gtcgtccagc aatttgtgaa
     7981 atcgttacag aatgagctca gcttcatgac ggaagggcaa aataccgagc aagttgcggc
     8041 caactttgag ggaaatgacc aagtcgtgat tcctaaaatt tactgggaat ggaccaagga
     8101 gcgcattagt gctcaggaat ttgtggaggg gattcccggc gtaaatattc aggccatcga
     8161 tgaagccggc atggatcgca aacgcattgc cctaaggggg ttccgattct aaatgtctct
     8221 gaatatcggt aagggcaggc aagccgtctt gcaatttcca aatacctgac agcgtcacga
     8281 gtagagtgtt atcggcagga gagctaaaag agagcttggt atgttcggat ggttgaacca
     8341 tggaagcaag gaacgcacga ttcaaacggt acgcctctta tggtagcgct aacgatagga
     8401 aagtggaata gactttgcgg tgcgccatta gtaattgaat tttcgaggtg ggcttacgaa
     8461 acagacttgg aaatggaccg gagggaatca aagacagtcc tgatatcctc cggattcatg
     8521 gatccagcct tcttcctgac acataaggga aggcaagagg ggctggatta gtgcggattg
     8581 ctttctgggt gccctgtaac ttttaacttc atggggtatg gccaggggga atattaatcg
     8641 agttatgcga atcgtttcgg cttcccgaat ttgctagaaa agtcatccgg atagtatttg
     8701 agaggagtaa agcaatcgtc gcaggtcgcg cttttcctac gcacgaggcc ggataataaa
     8761 tcaccgtttg aatcattcgg cttctctcga atgtttgaga ggaaaaaagg gaacagtatc
     8821 ctttctcggt agtgagcgcg actttatttg ccggtttagt agggtagatc ttcaggaata
     8881 gagaacgggg tggatacaac aggcttactc aagagcagca taccaccacc cggacggccg
     8941 atacgggcct tttcgtatat gtacgcgaag agcgcctcgg cttctgtatc caagaccact
     9001 acagtaacca tcctgactaa agtcgattcg ggcaaacggt cctttttggt caaagcttcc
     9061 cgaaggacag caatgccccg gcaggaaaca ctggcggcac gaatgatgtc cttttcttca
     9121 cgcaatatcc ggagtaatat cctgtccgtg ccgtcatcgg gaagaacgca ggtaatgatc
     9181 tgactggact caagaggcat ggaatggttg gagtcttgtc cggccctcat aatccttcct
     9241 tcgcagaacc cttattttgt aatcgttctt tcacggcctc tggaatatag attgcggcct
     9301 tttctaatgg cgtgatgtat aggaaacctc tggctggcgt atcgaactgg ccggccttaa
     9361 aaatgttgct ggccacaata tcggttcgtt cagaagagat caagacactg ataacatcct
     9421 ttgaggcgtc cacggcaaat cccagtattc ccaaacgttc gcgaatacct gttcctctac
     9481 catggtagac caaaactccc gttgctccgg catctcgcgc ggctttgact atcacgtcgg
     9541 cctcccctga ttgcaccacg catgtaatca tggctacgtc agtgagatat gtcatgttcg
     9601 tactcatttt atatagcttg aacctcctct acttgtgtgg aagcccttac ttcaagggcg
     9661 gcacgagctt gtgccttgac tttgtaatct gcccaaagac cggtcgccaa cacagtcaga
     9721 atggggccga tggaagccat ggaaagaatg ccaaagccct ccacggcgtg cgtagcattt
     9781 ccaaatccca gccccatggc taagaccagc gggacggtta tgggtccggt ggtgacaccg
     9841 gcactgtccc aggccacgtt gacgaaggct tcactggaaa aaatggtgag catgaacgcc
     9901 aagagatatc cgactaggat caaccaaact aacggcaaat caaaaatcag tttggctaaa
     9961 cccgtcgcaa tcccgaccgc aacaccaagg gagacggcca taatcaatgt ttttttctta
    10021 aacaccccgt tggtcagatt ttcggccgtt gtgccgagcg cgttcagggc cggttccgcc
    10081 actgtcgcac caaagccaag tccccaggca aataccagtg aaagaatcaa ccctaccgaa
    10141 tagacataaa taggtgatat tggcacgcct gagagggaca tgaatgcagc aggaaccagg
    10201 ctgcccacgt tccctcctaa ttgtgaaagt ccgtaggtca gtcccaagtt aaagacacac
    10261 atgccgataa tcgtcaggcc aatcccgtac agaatctcgc ccttattagg gagcgaatct
    10321 ttgagtatga gcctgaggac cacgaataga aacaggacga gtgggaggat ggcacgcaca
    10381 cccaacacaa tctccatacc agggctgcga gcataccaga cgggtgcaac ggattgacct
    10441 gctccggata cgtggttggc ggccgcaata atttcagcgg gtgttacggt ggatgcaatg
    10501 taaaaagcca ggctaagcac gccaatgacg gggaaaaggg aggccatagt tacaattccg
    10561 aaacccgata atcctgaatc tcctttcccg gctgccgttg cgatgccgat acccaatgcc
    10621 agaaccaatg gcacggtgac cggccccgtt gtcacggcgc cggcatccca ggccaaacct
    10681 agtacgttga tcagatccgg atcggatgcc ccatagagcg tcaaggcgag caccggagcc
    10741 agggacgcgt aaataaacgg cttaagactc cagccatata aaaaccgcaa ggtaccaatg
    10801 acggctgcta aacccacact gaacccgatc accagcacaa gggttccaga ccattcattc
    10861 aacaggacat acagataggc tgctttctca actacgacat tctggccggc gactttcaag
    10921 gccccgatag caggttccgc aaaagtgaca ccgatgccga gaagaagggt gataactaat
    10981 actaatggta aggatgattt ttctggcagg cgcttgccga tgatggtgcc aaaaggcatg
    11041 aggccaagct tgagaccttc catgaaaaac atcagtccaa cgatcacggc aaacaggcca
    11101 ctcatgatca tccaggagtt ctggaccagt tggcgcaaaa tcaagatctg gaacaacgcc
    11161 aagtatagcg ccagggggat gaccgatttg acctgttcca tgaatcgaac cgagacatag
    11221 gggcgtagga gtttgtaaat ttcctcagcc gtcagacaaa tccggagcgg ttggtagggg
    11281 atcaaatgcc cttgcgcgtc ccattccaaa tttggcttca tggaattgaa actgagttca
    11341 tcgtatccat gccccagtgc gcgttgataa tctccgaatc gataggaatc tgacattttt
    11401 ttctattggg gtagacctag agagtttccg gcagtatacc gttcataatc tttccaggaa
    11461 aggacaaaag caaaaagaga acgtttccct tttatttgat atgcgcatgg aacattcacg
    11521 gaaccagcct gagttcaaga aacatatttt ttataatcat ttcgtgatcg gggaaccgga
    11581 atagcaacct aagaagtcct ttggagtgaa atggcgatcc atctcgaatt caaggggtgg
    11641 gcgaccggta ggcagggaga agagcgcagg aggtaatgtt tgaatattat ctaaggcccc
    11701 tttgctgttc gatacggcct ggacacagcc ccattcagtg accgatggct ttcagggatc
    11761 acctcgacac gcgagttccg aaaatgtgtt aaaaattttg tgaatattag gtttaggttt
    11821 agtgggcccc ccctggtcct tgttccccag tccgtgccgg tcaatgctcg cggcgggcga
    11881 aatagaaata tgcgcccacg ctgataaaat ttgtcaccac agcgatgata ttccaaagga
    11941 tcacgggtct tgaaaggatc aacaacccgt agtagaccca tacgatttgg aagacgccca
    12001 taatggcagc catcctcgga ttcatgcctt tgcttgatct ccgcttaagc atcccgatca
    12061 aatccggtag ggcggcaaag gtcgttccta atcccgccac aaagccgatg acttcagacc
    12121 ttcggaagct ggggagtagc agactctccg tatcgtgtat gcctaattcc ccacacccag
    12181 caaggatgat catgctggaa gagaaacaaa gcaattgaag tctcttagaa gtaaccatgt
    12241 ttgtctccgt ttcacgcgac tcaggtccaa atgctcacct tgaagacgtg ataagcccac
    12301 ggcttcaggt ccacatagaa gcccgatgat tgaagttcgg ggcccactcg atcatcccca
    12361 tccagaccac cggtccttgg gttcatcatc accacaagat cgacggtgag agtttcgcga
    12421 gcccactttt gtaacgccgg cttttgggcg aacgcggtgt tcacgaaaag aaaattacag
    12481 tagggcagac gcctagcacg ccggccaacc gtcaggacag ctcgaagacg tgatagcccc
    12541 aggcagggat atcgagatag aagccccgtg ataggagttc gtcgccatcg cgttcgtaac
    12601 aggcgtcacc catctgatcc ttgagccgaa ccttgttgcc ggccaagtct ggaaacggga
    12661 gccggacata acactggctc tgggtggcag catagttcac cgtcaccaat agccgctcct
    12721 tacgggggcc ttgccaggca aagatcagga aggaatccca tgtcccgttc ccatcccacg
    12781 caggcacagc gtcgagcaag caccactggc cgtcccgtac ggcccgctgc cggagcaccg
    12841 cgagaaggcg atcgtagaag cggtgcagcg tgtcgttggt cggctccagc ggggcacgca
    12901 ccagatgcgg cgagatacgc ttccttcggc cttcaaactg cccctgatgg aaaaagcgca
    12961 tgcctgtgga caagaacgtg atgatggctg cggcctcatg cacgccgggc gcgaaggtcg
    13021 tggcggccct gggttcgtca tggttttcca ggaagcgggc cattttgttc tggtaggtag
    13081 gatcggcgcg taaatggtcg cgcaccgtcc tggcatgccc ttcgcggaga cgatcgtaaa
    13141 gccgtttgtc gtaggtgtag tcgaaaccct gttgctgcag ggtccattcg cgatcccaat
    13201 agacctccgc catgaaacag aagtcgggaa attgttttcg aactcgttga gttgctttcg
    13261 gccagaacaa ttccgcttgg aggccccacg tccgttcgaa gacatcgggt aggacgagca
    13321 ttgccatgtc gcaacgcacg ccgtcacact gtccggagat cctcagcaac tctccgatca
    13381 tcgcttcttg cgtttcggga ttgccatagt tgagttgtaa ggtgtccggc cagccctcaa
    13441 agtacgggtc tcgcccatag gcgagtacca gcggcccatt tttcgtatgc acgcggcaat
    13501 agtttcgggg tgagcgggtc aaatccgtct cactgccatg gacaaagtat tccgggtgct
    13561 cgttgatcca cgggtgatcc ggggccatgt ggttggggac gaagtcgagc atcagcttta
    13621 gtccgcgtgt gtgcagccgt ttccccaaac gggcgagcgc ctccgccccc cccagatcgc
    13681 gatgcacggt gtagtcttga atcgcgaatc ctgagccggc gatatcctcg tccttgagat
    13741 ccggcagtgt ttcggcgaat tcgcgacgcc atccgggatt cgcgcgcgag atcgcctgtg
    13801 ccgccgggcc cgtctgccaa acgctcagga accagatcca gtcaaaaccc aacttggcca
    13861 ggcgatccag ttcggtgtcc ggagtgtcgt ccaaggtcgc cggccggccg attgatttgg
    13921 acagatcagt cagccacacg cgggtgttga tctgatagat caggggatgt gcggagaagg
    13981 ccttcatgat tttgcgcggc tcccaacggt ctccttaaac gcacctcgct ttcccgtttc
    14041 caggaccagc ttggcgtcga ggaacccgta gagctggatg gttttggcca cgagcccggt
    14101 ccaccctgtc tggtggctcg cgcccagtcc cgccccgttg tcgccgtgaa agtattcata
    14161 aaagagaagg tggtcgcgcc agtggggatc ggattggaac ttttccgtgc ctccatacac
    14221 aggtcgccgg ccacgttcgt cccgcaggaa gatccggctc agccggtctg cgatgtcctt
    14281 ggagacttcg aagaggttca tcatcttgcc ggacccggtg ggacattcga ttttgaagtt
    14341 gtccccgtag taaagataga aattcaggag agcccggata atcatgacat tcaccggcat
    14401 ccaaaccggt cctcgccagt tggagttgcc gccgaacatg ccggtattgg attcccccgg
    14461 caggtaatcc acccggtact cctggccttg cacgtggaag acgaacgggt gctgctcgtg
    14521 gaacttggag atcgagcgga tgccgtaagg gctcagaaac tcgttctcgt cgagcatttt
    14581 tgttaggatc cggcgcagcc gatccgggtt gaccagggcc atgattccgc gttcggccat
    14641 gccgtaatga cccgggcctg tagggtgaat ggtcttaata agctcgggta tccggcgtat
    14701 gcgttcagcg atctgggcca gcgcctccgg aatggattcc cgctgcgact tttcgatgac
    14761 cgtagtggcg cacagaggca ggagccccac catcgacctt atgcgaagcc tctgggcgct
    14821 gccgtccggc agccgcagca ggtcgtagta aaagccgtcc tcctcatccc acatgccgtc
    14881 ccggccagga cggttcatgg ccccggcgat gtagtagaaa tgctcggcga acttttggac
    14941 catgtcctcg tagatcgggt catgggcggc gagttcgatc gcgagctcta gcatattctg
    15001 gctgaagaga gccatccagg ccgtgccgtc cgcctgctcc aggtggccgc cggtgggcag
    15061 cggggcgctg cggtcgaaga ccccgatgtt atcgaggccg aggaatccgc cctcgaacac
    15121 gttcttgccg aaccggtcct tgcggtttac ccaccaggtg aagttcagca ccaatttgtt
    15181 gaacgtctcc ctgagaaaat ccaggtccgt ctctccactc agggcctgct cggtgcggag
    15241 caggaacaag gtcgcaaagg catggaccgg gggattcaca tcgctgaagt tccactcata
    15301 ggcgggcatc tggccgttgg ggtgcaggta gacgcccttg agcatcaatt gcatctgctg
    15361 cttggcgaaa tccgggtcca cgatcgaaag cggcagggtg tgaaaggcca gatcccaggc
    15421 cgcataccag gggtattccc act
//
LOCUS       DATIAL010000137         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_561437, whole genome shotgun sequence.
ACCESSION   DATIAL010000137 DATIAL010000000
VERSION     DATIAL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561437"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..717)
                     /locus_tag="VLA60_04365"
     CDS             complement(58..717)
                     /locus_tag="VLA60_04365"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSF08623.1"
                     /translation="MDLHELKSSLHDCQRCGLSSGRTQVVFGTGHPQADIMFVGEAPG
                     FYEDRQGEPFVGAAGKLLNELLQSIGLSRADIFIANVIKCRPPNNRDPLPEEIETCKP
                     FLLQQIELIKPKLVCTLGNFATQTLLERKVGITKVRGQVIRMPNFIVFPLLHPAAALH
                     QGNLRIPLKEDFQKLKTVLEEMSKTPTLPQTASPPTLSTSNASSKEETSAEPPTQMSL
                     F"
     gene            complement(754..2142)
                     /locus_tag="VLA60_04370"
     CDS             complement(754..2142)
                     /locus_tag="VLA60_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF08624.1"
                     /translation="MTHHILILSGNLPGLLAAYRLIPYGFRITILEDHNPDSPAPSFP
                     HFAKPIEPDHPPSLQRLTTQPFPLILPRYYHATWALFQELAFEHATQCIQPVNLEFGT
                     SEGQTLTLSDAKGIATLHPMIRLAGFRALPWSDRWHLINFLEKKWEEPRSPDHHPDML
                     TVESWLISAQQSALARQRIWNPFCRLFLGCDVTQASLGYFLEVLSRFWLSKPNGPETF
                     LASPDMQSHLQHKLRHVLLEKGVTFYPSNAITGIHADTERIQAIGLAKGERLNADIYV
                     SPLSPLELLRLLPERAPARFSCFSHLAQLQESSGTLVQLTLNDTLLPPRLILNSGVFD
                     WVTSQAVPYTDGPTTLITGISLSSSPSALHTNDWLRETVWPHINKIFNISPKPSLPYS
                     GSSDTPSVYRYTPCLTGFRTFRPLSNTPIPNLFLAGPWTATPLPPSLESTVLSAYACA
                     RAVTELVQASSH"
     gene            complement(2139..>2560)
                     /locus_tag="VLA60_04375"
     CDS             complement(2139..>2560)
                     /locus_tag="VLA60_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HSF08625.1"
                     /translation="LGLAFQLTNILRDLKGDAEQNRIYLPLEDLQRFSYSENALLHQQ
                     GSPALVELLKFECERARTYYRQAQEILQTLPPSDQKSLVVAEIMHGVYSRILKQLEDP
                     HYQVFGPRVRVAPLQRLGIAAHIWIRSFLSHNIAPTV"
BASE COUNT          623 a          517 c          789 g          631 t
ORIGIN      
        1 ggacctcggc tgctgggccg aaacccagca tctgctagta aattgtttac atggtgtcta
       61 gaacaggctc atttgtgtgg ggggctcggc tgaggtctct tctttggagg aggcgttgga
      121 tgtggaaagg gttggtggtg acgcggtttg cggaagcgtg ggagttttgc tcatttcttc
      181 cagaaccgtc ttgagttttt gaaaatcttc cttgagagga attctcagat tgccctggtg
      241 gagtgcggct gccggatgca gcagtgggaa cacgatgaag ttgggcattc ggatgacctg
      301 cccacggact ttggtgatac cgacttttct ttccaataac gtttgggtgg caaagtttcc
      361 taatgtgcaa accaatttag gtttaatgag ctcgatttgt tgaagcagaa acggcttgca
      421 cgtctcgatt tcctctggaa gaggatctcg attgttgggt ggcctgcact taatgacgtt
      481 ggcaataaaa atatccgcac gactgagccc gatggattgt agtaactcgt tgagtaattt
      541 tcctgcggcc ccgacaaaag gctctccctg ccggtcttca taaaagcccg gggcttctcc
      601 gacaaacatg atgtcagctt gaggatgccc tgtcccaaac accacctgcg tgcgtcctga
      661 agacaagcca catcgttggc agtcgtgaag ggatgatttg agttcatgaa gatccattgt
      721 gagaggggat cttaggtttg acgatttagg atgtcaatgt gaagaagcct ggaccaattc
      781 agtcacagcc ctggcacagg cataggcact caggacggta ctttccagag aaggggggag
      841 tggggtggcc gtccagggac cggcaagaaa gagattgggg atgggtgtgt tggacagagg
      901 acgaaaggtg cggaaaccag tcaggcaagg agtatagcgg taaacggatg gcgtgtctga
      961 tgacccacta tatggcaatg acggcttagg ggaaatgttg aatatcttat tgatatgagg
     1021 ccagacggtt tctctcagcc agtcgttggt gtgtaacgcc gagggtgaag aagagaggct
     1081 gatgcccgta atcaacgttg ttggaccatc cgtgtatgga acggcctggc tggtgaccca
     1141 atcaaataca ccggaattaa ggataagccg aggaggaaga agtgtgtcgt taagggtcaa
     1201 ttggaccagt gttcccgatg attcctgcag ttgagccaaa tgggaaaaac acgaaaaacg
     1261 tgctggggct ctttccggta agagtcgaag tagttctaga ggggaaaggg gcgagacata
     1321 gatgtcggcg ttaaggcgct cgccttttgc caggccaatg gcttgaatcc gttcggtgtc
     1381 ggcatggatg ccggtgatgg cgtttgaggg ataaaacgtg accccttttt ctaataagac
     1441 atgtctgagt ttgtgttgaa gatggctctg catgtcaggt gaggctaaga aggtttctgg
     1501 tccattcggt ttggataacc agaatcgaga aagtacttcc aggaaatacc cgagtgatgc
     1561 ctgggtcaca tcacacccta aaaacaatcg acagaatgga ttccagatac gttgtcgtgc
     1621 aagagccgat tgttgagcgg aaatcagcca ggattcaacc gtgagcatgt cagggtggtg
     1681 atctggtgaa cggggttctt cccatttttt ttctaaaaaa ttaatcaagt gccagcgatc
     1741 tgaccaggga agggcgcgaa agccggcaag tcggatcatg gggtgaagag tcgcaatgcc
     1801 cttcgcgtcg gagagggtga gtgtttgacc ttcggatgtc ccaaactcca ggttgaccgg
     1861 ttgaatgcat tgggttgcgt gctcgaaagc cagttcctga aaaagtgccc atgtggcatg
     1921 gtaataccgt gggagaatga gtgggaaggg ctgcgtcgtg aggcgttgaa gggatggtgg
     1981 atgatccggt tctatcggtt tcgcaaaatg gggaaatgat ggtgccggag aatcagggtt
     2041 gtggtcctcg agaatggtga tccggaatcc gtaggggatg agccgatagg ccgcgagtaa
     2101 tcctggcaga tttccgctca gtatcagaat gtggtgtgtc acactgttgg agcaatatta
     2161 tgggaaagaa aagaccgaat ccaaatgtga gcggcaatcc cgagtcgttg taggggagcg
     2221 actcgaaccc gtgggccgaa tacctggtag tgcgggtctt cgagttgttt gaggatgcgg
     2281 ctgtagactc catgcatgat ttctgcaacc accagcgatt tttgatcgga ggggggcagg
     2341 gtttggagaa tttcttgtgc ttggcggtaa taggtgcgag ctctttcgca ttcaaatttc
     2401 agaagttcaa ccagcgccgg cgacccttgt tggtgcagga gagcattttc cgaataacta
     2461 aaacgctgta agtcttcgag cggaagatag atgcggttct gctcggcatc ccccttgaga
     2521 tcacgaagaa tattcgttaa ttgaaatgcc aatcccaagt
//
LOCUS       DATIAL010000138        11846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1099080, whole genome shotgun sequence.
ACCESSION   DATIAL010000138 DATIAL010000000
VERSION     DATIAL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11846
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099080"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VLA60_04380"
     CDS             <1..743
                     /locus_tag="VLA60_04380"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF08626.1"
                     /translation="SIEVTKSKPASFFVPLLMMNTNMLEACRLNKVHRVVYTSSIGAY
                     PSAEIFKEQEGEDEGPPMDMYPGWAKRMAELQVRAYQIQYSLTHYAVVRPCNVYGPGD
                     NFDPANAMVIPTLMRRIFSKEDPVLIWGDGTAIRDFAYSGDVADGVILALYHGTGGRY
                     VNLGSGIGYTIGELVESLQSVVPFRAQFDHTKPTGFPRRVMDISLAKHLLGYAPQISL
                     QEGLRHTWDWYLEHHDEYLQRQNYFAAS"
     gene            703..1260
                     /gene="rfbC"
                     /locus_tag="VLA60_04385"
     CDS             703..1260
                     /gene="rfbC"
                     /locus_tag="VLA60_04385"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSF08627.1"
                     /translation="MNIFNGKTISPPPNLQISSCRLEGVRLVTPPTIFEDFRGTYVET
                     FNEDLYRQAGIDMPFVQDDISVSGRHVLRGVHGDQETWKLVSCLWGKFYLVVVNWDPT
                     SPQYQQWESFTLSDQNRQQVLIPPKFGNAHLVLSDQAIFHYKQTTNYNREGQFTLPWN
                     DPALGIWWPVKRPIVSQRDEGVAHV"
     gene            1253..2266
                     /locus_tag="VLA60_04390"
     CDS             1253..2266
                     /locus_tag="VLA60_04390"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF08628.1"
                     /translation="MFKGSKVLVTGAGGFLGTNMAKRLVEEGCHVRGTLHARRPQFEH
                     PHLEYVSADLTNMEDCRRVVEGMDYVFMCAANTSGAAVMATQPLAHVTPNVVMNAQML
                     EASYQAKTKKFLFISSSAAYPPSGERPVSEAEMFAGDPYEAYFSVGWMKRYGEVLCRM
                     YSEKIKEPMKTVVVRPSNVYGPYDKFDFERSHVTAALIRKVVERQDPLEVWGTGEDVR
                     DLLYVDDFIDAVMLAMARLDRFDPVNIGYGKGCRVRDVLNAILEVDGFKNAHVVVNPT
                     KPSMIPIRLLDVEKARVQLGFSPKFNLQAGLKKTLRWYREQIETQGRVEPVQFSAAHI
                     RAS"
     gene            2292..7490
                     /locus_tag="VLA60_04395"
     CDS             2292..7490
                     /locus_tag="VLA60_04395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF08629.1"
                     /translation="MKHIFPESSMAHRWLDGLKGLEVGPSSHNPFGLETRTVGLPDEI
                     YHQEQIALVGQAAPLDILAEADAIPVPSESEEFVLSSHVIEHCPNFLKTLWEWYRIIK
                     PGGLLYLIAPHPDAAPGDRGKPLTDWGHVVQDYVFNATAETEPEAGRFLHCHYHIFSP
                     ETMKEFFFRFFGERLILVDSREKDDKIGNGFALVYQKHDSLIHAYSWSLTCQGKTVSI
                     PKPADFYLLKESAHSSSLVLSHVEAQVTAQTDSVLSFQGPTIQNSKTVSPRVSVIVAA
                     YNAERFMRGALVDLEEQTIANEIEIIIVETGSQTEEYKIITEFQARFSNIIYVRISAR
                     ENPASACNRGIQMARGQYITLAPTDDRRRVDALEILAAKLDAHQEVALVYGDVFVTNF
                     ENQRFVNHIRCGYHIRPEFSPDIMLSGCHMGPQAMWRKSVHDEVGYFDETLPSATDYE
                     FWCRIAERYPMKHISSFLGVYYENPGGIINSNPERAHRETRNVQQAYRDKFPVPKAQN
                     INNYQFQGKIKTGKFVNICMVTFNRLEFTKQSITGLFQHTCFPHVITVVDNGSTDGTP
                     EYLQALLKEGFITNLILLPENVGIAKASNLAWSQEPESEYYLKLDNDIVIQKPGWLSN
                     MVDVIDHIPELGAIAYSFEPVSYPVRETRGVRIRPKDGNVNGACTLIPKRTHQILGFW
                     CEDYGLYGEEDADYGLRIHLHGLQNAYMEDEKVGLHLPAGRAAHIDAQTLKATDGIEE
                     EIHAEYREFKDGCRRNNVQNIAKKNCQAYQTGVKPLFSSSSFVKEFHKNREHCERSHG
                     PTRQRIQVEELPREMILERAKDSSPECLPTVSVIASDYICGRIRLLQPLLAGEGIWGK
                     GPIVGDANILLGDVTLPVMGAGQIWAIQRNCRLTEEILKEAQSLGTRIVYDCDDLLWE
                     IPVDNPNHGYFTPECLQRMFGLMYMADCVTVSTEPIQTDLYKRGIESMVLLNCLIRQE
                     WEDFKPQRQTGSRPRVGWAGQASVHKCDTAILETIIEALKDEVEWAFLGDAPRMAMSP
                     GVISETFSMVDVAQYPKKLASLNLDLALAPLVPNPFNEAKSDIRILQYGILGYPVIGT
                     DIFPHQHAPIIRLPHEPQAWIKAIRERIHDLGALEREGDHLRAWVLKHRLMEHVLPQY
                     EQAWVGRDVKSSKGVPQSKIQSLPVPSYSPRNRGEYSFDCSIIIPVWNKVELTRQCLT
                     HLAEVTDGCSYEVIVVDNASTDETPAFLSSLSGDIQVITNPANYGFAKACNQGAASAK
                     GRFLVFLNNDTIPKPGWLRALLNEVESHQDVAIVGSKLLYPNNTIQHAGVVFAKHCLT
                     PYHIFRGAPGDLRAANVRQEFQVVTAACMLIRREEFNAVGQFDEIYQNGFEDVDLCLK
                     VGKRGKKVIYQPESVLYHLEQQTPGRKDHEAERHNGIVLMERWASSIVVDEDFHTVPA
                     GYANRYSLRDGRLQPSLQALQGDREITQWKRVQRVQEVQLRQRHAAGEALISWDNREL
                     YTLLSECRDWPDDGEVLRWAAKLCQSLQLSDGERAFLKRILSMGEDRAALERLAKLAL
                     SIKDLSEATDQIQALLQSDPSNGSGHWLQGILLMQSQQCAEAALSFRRALQCGADSRK
                     TSIGLGMACMGMGDFEEAWRIFEQIVADYPDDVEAMNGLIQAGTSLERWKGLGDRLGR
                     YVERNPANCDMRFALAGVEFRASRLDVAKQQFEMLSLLKPDYEGLHDLEILLQKVPLD
                     GHALAT"
     gene            7524..8759
                     /locus_tag="VLA60_04400"
     CDS             7524..8759
                     /locus_tag="VLA60_04400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSF08630.1"
                     /translation="MSRALLIQLARLGDLVQSLPVLTSLQTAYPERPLDLLCPSPLVG
                     LGELFPCVDRVFPWNGEQWHELAKTKMTDCDQQLALATRYLADRAFPPYALAYNLNNH
                     PRGILAAHVLSGMVVGPGEHGPLNQTLPAWAGYLRRIAQDRGSNRVHLADAFCGLCQV
                     FPPLDVPHLKAPEEAWPFELELPVNGEPLTLIGIVLGAGDADRRVPLSVWQDLIVACA
                     EHIPQCYLLLVGGAGEREAALALEHRLPAQYLNRVVNGCGRTSLPQLVALLNRCQWVV
                     GSDTGPLHLGTMCGARAIGWYFSQARVHETGPYGVGHYVWQRAGARLWESQDVRDARA
                     VGSSTAYWPVMETVNLIREDTVNTTTDEWDLWTSNRDEWGAFYTRDGLPDEAVLRRKD
                     TWEALSQLANQGIRMEVSK"
     gene            8827..10644
                     /locus_tag="VLA60_04405"
     CDS             8827..10644
                     /locus_tag="VLA60_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF08631.1"
                     /translation="MDVLKQNIDLLRDQDPILAAQILQVPGGTLSIQPSKSGMPTALV
                     NSRYLHSAYDPVREAERWAEERVKDCQAGETIVLLGVGMLYHVQALRQMLPRDQVIMV
                     VVPDLSEFADCVSVRALEGWGEGVMWVTGSTADMAVQVTEKAKRVRVLTYEPAATVHR
                     ETYEHFRLQLREHLAHQLSGTLHIMIVGPIYGGSLPIARYVVNALEGLGHRVSWVDHS
                     PHYAGYQNLETIHDHRLRLTVQQRMSETLAVISLAHVAEDPPDLVLALSQAPLTMAVL
                     EQMRRKKVLTAMWFVENFRHLTYWQQMVTGYDFWFVMQQAACLDAFRKAGAKQVSYLP
                     LAADPAIHQPMAFTEEERREFGADVSFLGAGYRNRRHILPSLVGHEWTFKLWGNEWDN
                     PGVLAPVLQRGGTRIDTSTSVKIFNATSVNINLHSYTGDGFDPEGDGVNPRTFELASC
                     GAFQVVDTRTLLPALFDESMMAVINNPGQLLPTVQTYLHDPARRVAMAELSRKRVLEA
                     HTYGHRMKTFLGAVGMASPDRLGGILQGDRHAESLVARSGKTPELIPMLKQFPPTDRV
                     ELVDVAKSIRNKGPEARLNREELLILMMDEYRQEKRDFL"
     gene            10684..>11846
                     /locus_tag="VLA60_04410"
     CDS             10684..>11846
                     /locus_tag="VLA60_04410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSF08632.1"
                     /translation="MNKQVLIINITRMGDLIQMVPLLSRLEEEWPGVAIDLIVDKEFA
                     HVASLIPGLRQVLMFDFQLLMDESRVRARDVVALYRELAGWVQPLLQVGYDRVVNLTF
                     NRRSAFLVKYFGCADERGMTTTQDGSFVVKNPWMKYFVDFHVYRQLNRFNIVDLFSLG
                     GSGPGSFSPLRLSVPQHMKEWARTYVRQAGTANEWIGIQVGASDPMKAWLPEYFGQMM
                     AYLSQKRPVGFVLIGTKKEEADVQEAIRCYRQAGGLGNICEAVGQTTVPQLVALLEHC
                     VLMVTNDTGPMHMAVGVNTPVLNVSVGHVNFWETGPFGPGHWVVQPEIICGPCGFDKV
                     CPHHACKDQIIPQEVAELCLHLLGKRPFPSFSSKIRVYEGGVNEDQLGTFMIRA"
BASE COUNT         2886 a         2602 c         3258 g         3100 t
ORIGIN      
        1 gttccattga ggtcacgaaa tccaagccgg caagtttttt cgtcccgttg ttgatgatga
       61 ataccaatat gttggaggcc tgtcggctga ataaggttca tcgggtggtc tatacctcat
      121 ctattggagc atacccgagt gcggaaattt tcaaggaaca ggaaggagaa gatgaagggc
      181 caccaatgga tatgtaccct ggctgggcca aacgaatggc tgaattgcaa gttcgtgcct
      241 atcaaataca gtacagtctg acgcactacg ccgtggtgcg tccctgcaat gtgtatggac
      301 caggagataa tttcgatcct gccaatgcca tggtaattcc caccctcatg cgacgcatat
      361 tttctaaaga agatccggta ttgatttggg gagatggcac cgccattcgg gattttgcct
      421 atagtgggga tgtcgctgat ggagtgattc ttgctttgta ccatggaacg ggtgggcgtt
      481 acgtcaattt aggcagtggt atcggctata ccattggaga attagtcgaa tctcttcaat
      541 cggttgtgcc gttccgggcc caatttgatc acacgaaacc caccggattt ccccggcgtg
      601 tgatggatat ttctttggca aaacatcttc tggggtacgc accacaaatt agtcttcaag
      661 aggggttacg gcacacatgg gattggtatc tggagcatca tgatgaatat cttcaacggc
      721 aaaactattt cgccgcctcc taatttacag atctcttcct gtcgcttgga aggagtgaga
      781 ttagtgaccc caccgacaat ttttgaagat tttcggggaa cctatgttga aacttttaac
      841 gaggacctgt accggcaagc tggaattgat atgccattcg tgcaggatga tatctccgtg
      901 tctggcaggc atgtgttgcg gggcgttcat ggagaccaag agacctggaa gctggtgtca
      961 tgcttatggg ggaagtttta tttggtcgtc gtcaattggg atcccacctc tccacagtat
     1021 caacagtggg aatcgtttac gctatcagat cagaatcgtc aacaagtctt gatcccaccc
     1081 aagtttggaa acgctcatct ggtgctgtct gaccaggcca ttttccacta taagcaaacg
     1141 accaattata accgcgaagg gcaatttacc ttaccttgga atgatccagc tttgggaatc
     1201 tggtggccgg tcaaacggcc gattgtttcc caacgggatg aaggagtggc tcatgtttaa
     1261 gggtagcaaa gtattagtga cgggggcggg aggatttctg ggaaccaata tggctaagcg
     1321 tttagtggaa gaggggtgtc acgtgcgtgg gacgttgcat gcacgtcgcc cacaatttga
     1381 gcatccgcat ctggaatatg tcagtgcgga tttgaccaac atggaagatt gtcgccgggt
     1441 cgtagagggc atggactatg tctttatgtg tgcggcgaat acctcgggag ccgcagtcat
     1501 ggcgacccag ccattagccc atgtgacccc gaatgtggtg atgaacgctc agatgttgga
     1561 agcttcctac caagcaaaaa caaagaagtt tctctttatc agtagcagtg cagcctatcc
     1621 acctagcggg gaacggccgg tctccgaggc ggaaatgttt gccggggatc cgtatgaagc
     1681 ctacttcagt gtgggatgga tgaaacggta tggggaggtc ctgtgccgaa tgtattctga
     1741 aaaaattaag gaaccgatga aaacagtcgt cgtgagaccg tctaatgtct atggaccata
     1801 tgacaaattt gattttgagc gttcccacgt gaccgcggca cttatccgta aagttgtaga
     1861 aaggcaagat cctttggagg tgtggggaac cggagaagat gtcagagact tattgtatgt
     1921 ggatgacttc attgacgccg tgatgttagc aatggctagg ctagatcggt tcgacccagt
     1981 gaatattgga tatggaaaag gttgtcgagt tcgggatgtg ttgaatgcca ttttggaagt
     2041 tgatgggttc aagaatgcgc atgttgtcgt gaatccaacg aaaccttcta tgattcctat
     2101 tcgcctgctt gatgtggaga aggcaagagt ccaattggga ttttccccaa agttcaatct
     2161 ccaggccggg ttaaagaaaa ctttacgctg gtatcgggaa cagattgaga ctcaggggag
     2221 ggtggagcca gttcagttta gcgcagccca tattcgagcc tcctaatgat ctggatgaat
     2281 ctgaagcgcc tatgaaacat atctttcccg aaagttcaat ggctcatcgc tggctggatg
     2341 ggttgaaagg tttagaagtt gggccctcct ctcataaccc atttgggtta gagacgagga
     2401 cggtcggact tccagatgaa atctatcatc aggagcaaat tgctctagtt ggacaagcag
     2461 ctccattaga tatcctggct gaggctgatg ccattccggt tcccagtgaa tctgaagaat
     2521 ttgtgttgtc gtcccacgta atcgagcact gtcccaattt tttgaaaacg ttgtgggaat
     2581 ggtatcgaat cattaagccg gggggattgt tgtatctcat cgctccacat ccggatgctg
     2641 ccccagggga tagagggaag cccctgactg actgggggca tgttgtacaa gattatgtat
     2701 tcaatgccac ggcagagact gagccggagg ctggacgatt tcttcactgc cattaccata
     2761 ttttttcacc agagaccatg aaggagtttt ttttccgatt ctttggagag aggcttattc
     2821 tggttgatag tcgggaaaaa gatgacaaga ttggaaatgg ttttgccttg gtctatcaga
     2881 agcatgattc attgatccat gcgtattcct ggtctcttac ctgtcagggg aaaacggtgt
     2941 ctattcccaa gcctgcagat ttctatttac tgaaggaatc tgcacatagt tcttcccttg
     3001 ttctatccca tgtggaggca caggtgaccg cacaaaccga ttccgttctt tcctttcaag
     3061 gtccaacaat tcaaaattcg aaaacagttt ccccccgagt ctccgtcata gtggcggcgt
     3121 ataatgccga acgatttatg cggggagcat tagtagattt agaagaacaa acgattgcga
     3181 acgaaattga aattattatt gtggaaaccg gttcccaaac tgaagagtac aaaattatta
     3241 cagaatttca agctcgattc tctaatatca tttatgtacg gatcagtgcg agagaaaatc
     3301 ccgcttcagc ctgtaatcga ggaattcaga tggctcgcgg tcaatatatt accttggctc
     3361 caaccgatga tcgacgtcgg gtagatgccc tggagatctt agccgcaaag ttggatgctc
     3421 accaggaggt ggccctggtc tatggagatg tgtttgtcac gaattttgaa aatcagcgat
     3481 ttgttaatca tattcgttgt ggctatcaca ttcgtcctga attctcacca gatatcatgc
     3541 tttcagggtg tcatatgggt ccgcaggcta tgtggcggaa atcggtccat gatgaagttg
     3601 ggtattttga tgaaacgttg ccgtcggcta ccgattatga attttggtgc aggattgccg
     3661 aacggtatcc catgaaacat atatcctctt ttctcggggt atattacgag aatcctggcg
     3721 ggatcattaa ttccaatccg gaacgtgctc atcgggaaac ccgaaacgtt caacaagcct
     3781 accgggataa atttcctgtt cccaaggcgc agaacataaa caattatcaa tttcaaggca
     3841 aaatcaagac tgggaaattc gttaatatct gcatggtgac ctttaatcgg ctggaattca
     3901 cgaagcagtc cattaccggg cttttccagc atacctgctt cccacatgtc atcaccgttg
     3961 tggacaacgg aagtacggac ggcacgccag agtatttaca agcgcttctc aaagagggat
     4021 ttattaccaa tcttatcttg ttgccagaga atgttggaat tgccaaggct tcgaatttgg
     4081 cgtggagtca ggagcctgaa tcagagtatt acctcaagct ggacaatgac attgtgatcc
     4141 aaaagcctgg ctggttatcc aatatggtcg atgtcatcga tcatattcct gagttgggtg
     4201 ctattgccta tagcttcgaa ccggtgagct atcccgtccg tgaaacacgg ggagtgcgca
     4261 ttcggcccaa agatgggaac gtaaatggag cttgtactct tatccccaaa agaacccatc
     4321 aaattttggg gttttggtgc gaggattacg ggttgtacgg ggaagaggat gcggattatg
     4381 ggctgagaat ccatttgcat ggtttgcaaa atgcctatat ggaggatgaa aaagtgggat
     4441 tgcatctacc tgctggcagg gcagcccaca tcgacgcaca gacgcttaag gccactgatg
     4501 gcattgaaga agagatccac gctgagtacc gggaattcaa agatggatgt cggcgaaaca
     4561 atgttcagaa tatcgcgaag aaaaattgtc aggcttatca aactggtgtg aagcctctgt
     4621 tttcttcgtc ttcctttgtt aaagaattcc ataaaaatag agaacactgc gaacgatctc
     4681 atgggccaac ccgccaaagg attcaagttg aagaattgcc tagagaaatg attcttgaaa
     4741 gggctaaaga ttcttctcca gaatgtttac caaccgtttc cgtcattgcg tctgattata
     4801 tttgtgggcg tattcgactt cttcagccac tcctggccgg tgaagggatc tggggaaaag
     4861 gccccattgt cggcgatgcc aatattcttt taggggatgt tacacttccg gtaatggggg
     4921 cagggcagat ttgggctatt cagcgtaatt gtaggctgac agaagaaatt ttaaaggagg
     4981 cgcaaagcct aggtactcga attgtttacg actgtgacga tttgctctgg gaaattcctg
     5041 tcgataatcc gaatcatgga tactttactc ctgaatgtct tcaaagaatg tttggtctta
     5101 tgtatatggc ggattgcgtg acggtgtcta cggaacctat tcagactgat ttgtataaac
     5161 gcggaataga atcgatggtt cttctcaatt gcttaatacg ccaggaatgg gaggatttca
     5221 aacctcaacg acaaacgggt tcccgtcctc gagttggatg ggcaggacaa gcaagcgtgc
     5281 ataaatgtga tacggcgatt ttggaaacga ttattgaggc attgaaagat gaggttgaat
     5341 gggcattcct cggagatgca ccacgcatgg ccatgagtcc aggtgtgatc agcgaaacat
     5401 tttcgatggt agatgtggct cagtatccta aaaaattggc gagtctcaat cttgatcttg
     5461 cattggcgcc attggtgcca aacccattca atgaagccaa aagtgatatc agaattttac
     5521 agtatggcat attgggctat ccggttattg gcacggatat ttttccccat caacatgcgc
     5581 ccatcatacg gttgccgcat gaaccacaag cctggatcaa ggcgattcgc gaacgaattc
     5641 atgatttagg tgccttagaa agggaaggag atcatctccg agcctgggta ttaaagcatc
     5701 gtcttatgga acatgtgctt cctcagtatg aacaggcctg ggtgggccgt gacgtaaaat
     5761 cctcaaaggg ggtgcctcag tcaaaaatac agagtctacc agtgccctct tattccccga
     5821 ggaaccgggg cgagtattcc tttgattgtt caatcattat tcctgtttgg aataaagtgg
     5881 aattaacccg tcagtgctta actcatttgg cggaggtcac ggatggttgt tcgtatgaag
     5941 ttattgtcgt cgataatgca tccaccgatg agactccggc atttctgtct tccttaagtg
     6001 gggatattca ggtcatcacg aatccggcca attatgggtt tgccaaagcc tgcaaccaag
     6061 gggccgcttc tgccaagggc cgttttctgg tctttttaaa taacgatacg attccgaagc
     6121 caggatggtt gcgggcttta ttgaatgaag tggagagcca tcaggatgtt gccattgtcg
     6181 ggagcaagct gctctatccg aacaacacca ttcaacatgc cggcgtggtt ttcgccaaac
     6241 attgtttaac gccctaccat atatttcgtg gtgctccagg agacctgcgt gcggccaatg
     6301 ttcgtcaaga gtttcaggtt gtcactgcgg cttgtatgct cattcgccgt gaggaattta
     6361 atgcggtcgg gcaatttgac gagatctatc agaacgggtt cgaggatgtg gacttgtgcc
     6421 tcaaagtggg caaacggggc aagaaagtca tctatcaacc ggaaagtgtg ctctatcatt
     6481 tggaacaaca aactcccgga aggaaggatc atgaggccga acggcacaat ggtattgtgc
     6541 tcatggaacg gtgggcttcc tccattgtgg tcgatgagga tttccatacg gttcctgcag
     6601 gctatgccaa ccggtattcc ttgcgtgacg gccggcttca gccctcgctc caggcattgc
     6661 agggcgatag ggaaataacg cagtggaaac gagttcaacg ggttcaggaa gtccaattac
     6721 gtcagcggca tgctgcaggc gaggctttga ttagctggga caaccgtgaa ttatatactc
     6781 tcctttccga gtgtcgagat tggccggatg atggtgaagt gcttagatgg gcggccaaac
     6841 tttgccaatc gttgcagcta tcagacgggg aacgggcatt tttgaaacgg atactgagta
     6901 tgggagaaga tcgagcggca ctggaacgtc tcgctaagct ggcgctcagt ataaaggatc
     6961 tatccgaggc gactgatcaa atccaggcgc tccttcaatc tgatcccagc aatgggtcgg
     7021 gtcactggtt gcagggaatt ctgttgatgc aatcgcaaca gtgtgccgag gcggccctgt
     7081 catttcgtcg cgctcttcaa tgtggcgccg attctcgaaa aaccagtatc ggtttgggaa
     7141 tggcgtgtat ggggatggga gattttgaag aagcctggag gattttcgag cagattgtgg
     7201 cggattatcc tgatgatgta gaagccatga atgggttaat tcaggccgga acatctctgg
     7261 aacgatggaa aggcttgggg gatcggcttg gaaggtatgt cgaacgaaat cccgcaaact
     7321 gcgatatgcg ctttgctctg gccggggtgg aatttcgtgc cagtcgactc gatgtggcca
     7381 agcaacaatt tgagatgtta agcctcttga agcccgatta tgagggatta cacgatttag
     7441 agattctcct gcagaaggta cctcttgacg gtcatgcgct tgcaacttaa atagaagaac
     7501 tttttgccta gtcaagttaa cctatgtccc gagccctctt aattcaatta gctcggctgg
     7561 gtgatctggt ccaatccctt cctgttctga cctctctgca aactgcctat cctgagcgac
     7621 ctcttgacct cctttgccct tcgcccctgg tgggccttgg agaacttttt ccttgcgtgg
     7681 acagggtttt cccttggaat ggggaacaat ggcatgaatt ggccaagacc aagatgacgg
     7741 attgtgacca acaacttgcc ctagccacac gctatttggc tgaccgtgcc tttcctccgt
     7801 atgcattggc ctataatttg aataaccatc cccgagggat acttgctgcc catgtattga
     7861 gtggtatggt cgtcggtcct ggcgagcatg gaccattgaa tcagacgctt ccggcatggg
     7921 cgggatattt acgtcgaatt gcgcaagatc gagggagcaa ccgggtgcat ttagcggatg
     7981 ccttttgcgg gttgtgtcag gtttttccgc cattggacgt tccccacttg aaggcccctg
     8041 aggaggcatg gccctttgag ctggaactgc cggtgaatgg tgaacctttg actctcatcg
     8101 gaattgttct tggtgcggga gatgccgacc ggcgagttcc cctttcggtg tggcaagacc
     8161 tgattgttgc atgcgctgag cacattcctc agtgctatct gcttttggtc ggtggtgcgg
     8221 gagaacggga agcggcattg gccttagagc atcgccttcc cgcccaatat ctcaatcggg
     8281 tggtgaatgg gtgtggtcgg acttcgttgc cccagcttgt ggctcttctt aatcggtgtc
     8341 agtgggtggt cggatcggat acgggaccat tgcatctcgg aacgatgtgt ggggctcggg
     8401 cgatcggctg gtatttctcc caggccagag tccatgaaac gggcccctat ggggttggtc
     8461 attatgtgtg gcaacgcgcg ggagcgcgac tttgggagag tcaggatgtt cgtgacgcac
     8521 gcgctgtagg atcatccact gcctattggc ctgtgatgga aaccgttaat ttaatacggg
     8581 aagacacggt gaatacaaca acagatgagt gggatctctg gaccagcaat agagatgagt
     8641 ggggggcgtt ttataccagg gatggccttc ctgacgaggc tgttctccgg cgaaaagata
     8701 cgtgggaggc gctgtctcag ctggcgaatc aaggtattcg gatggaagtc tcgaagtagt
     8761 cgaaaatgct ccggtagctt aaaggcaggg gctgtgtttc caggacaata aaccaggaac
     8821 agataaatgg acgtgttgaa gcagaatatt gatcttcttc gcgatcagga tccgattctt
     8881 gccgctcaga tcctccaggt accaggagga accctttcga tccaaccatc taaatctgga
     8941 atgcccaccg cgcttgtcaa ctcccgatat ctccatagtg cgtatgatcc ggttcgcgaa
     9001 gcggagcgct gggctgagga acgcgtgaaa gactgtcaag caggggaaac tattgtcctc
     9061 cttggcgtgg gaatgctcta ccatgtgcaa gccttgcgtc agatgttgcc ccgtgatcag
     9121 gttataatgg tcgtcgtgcc ggatctttct gaatttgcgg attgtgtctc ggtccgggcg
     9181 ctggaagggt ggggtgaagg ggtgatgtgg gtgacgggtt ccacggctga tatggctgtc
     9241 caagtgacgg agaaagccaa gcgggtgcgc gttctgacct acgaacctgc cgccacggtt
     9301 catcgtgaga cctatgaaca ctttcgactg cagcttcgcg agcatctcgc tcaccaatta
     9361 agcgggacgc ttcacatcat gatcgtcgga cctatttatg gcgggtcgtt gccgatcgcc
     9421 cgatatgtgg tgaacgcctt ggaaggcctt ggacatcgtg tgagttgggt ggatcatagt
     9481 cctcattatg cgggatatca gaatttggaa acgatccatg atcaccggtt gcggctcaca
     9541 gtccaacaac ggatgagtga aacgctggcg gtgataagcc ttgctcatgt cgcagaagat
     9601 ccccctgacc tagtgttagc cctgtcccag gcgccattga ccatggctgt tttagagcaa
     9661 atgcgtcgaa agaaggtgtt aacggccatg tggtttgtag aaaattttcg acatttaacc
     9721 tattggcagc aaatggtgac gggatatgac ttttggtttg tgatgcaaca ggcagcatgc
     9781 ttggacgctt tccgaaaagc cggcgccaag caggtctcgt atcttccctt agcagctgat
     9841 ccggccattc atcaacctat ggccttcacc gaagaagagc gacgggagtt tggtgccgac
     9901 gtctcgtttt taggagcagg gtatagaaat cggcgacata ttctcccatc cctggtcggc
     9961 catgagtgga catttaaatt gtgggggaat gaatgggaca acccgggggt gttagcaccg
    10021 gttcttcaaa gagggggcac gcgtattgat acctccacca gtgtgaaaat tttcaatgcc
    10081 acctcggtca atataaattt acattcgtat acgggagacg gattcgatcc ggagggggat
    10141 ggggtgaatc ctaggacatt cgaattagcc agttgcggag cgtttcaagt ggtcgatact
    10201 cgcaccttgc ttccggccct ctttgatgaa tcgatgatgg cggtaattaa taatccgggt
    10261 cagcttctcc caactgtcca aacctatctc cacgacccag ccaggcgagt ggccatggct
    10321 gaattgtccc ggaaacgggt attagaagcg catacgtacg gacatcggat gaagaccttc
    10381 ctaggcgccg tgggtatggc gagtcctgac cggttaggag gtatccttca gggggaccgt
    10441 cacgcggaat ccttagtggc tcgaagtggg aagaccccgg aattaattcc aatgctgaaa
    10501 caatttcctc caaccgatcg tgtggagtta gtggatgtgg cgaaaagtat tcggaataaa
    10561 gggcctgaag cccggttgaa ccgggaggaa ctactgattc tgatgatgga tgaataccgc
    10621 caggaaaaac gggattttct ctgaccctgt gatgggataa ttttccgtta ccaccaattt
    10681 ttgatgaata aacaagtcct cattattaat attacccgga tgggtgattt aattcagatg
    10741 gttccccttc tttcacggtt agaagaagaa tggccgggtg tggccattga tctaattgtt
    10801 gataaagagt ttgcccacgt cgccagccta attcctggcc ttcgacaggt gttgatgttt
    10861 gactttcagc tgctgatgga tgaaagccgg gtgcgtgcgc gagacgtggt cgccctctac
    10921 cgggaacttg ccggttgggt tcaaccgtta cttcaggtgg ggtacgaccg tgtcgtgaac
    10981 cttaccttta atcgtcgcag tgcgtttctg gtgaaatatt tcgggtgtgc agatgagcga
    11041 ggaatgacca cgacccagga tgggagtttc gtggtgaaaa atccttggat gaaatatttc
    11101 gtggatttcc atgtgtatcg acaattgaat cgatttaata tcgtggatct tttttcgctt
    11161 ggtgggagcg ggccgggttc gttttctcct cttcgcttat ctgtgcccca gcacatgaaa
    11221 gaatgggcac ggacctatgt gagacaagca gggacggcca atgagtggat tggcattcaa
    11281 gtcggcgcca gtgatccgat gaaagcctgg ctaccggaat actttggaca aatgatggca
    11341 tacctcagcc aaaaacggcc agtcggattt gtgttaattg ggacgaagaa agaagaagcg
    11401 gatgttcagg aggccattcg gtgttaccgg caggcgggag gattgggaaa catctgtgaa
    11461 gccgttggcc aaactactgt gccgcaactg gtggccttgc ttgaacattg tgtcttgatg
    11521 gtgaccaatg acacggggcc catgcacatg gctgtgggag tgaatacgcc ggtcctgaac
    11581 gtatcggtgg ggcatgtgaa tttttgggaa accggaccgt ttggaccagg gcattgggtg
    11641 gttcaacccg agatcatttg tggcccatgc ggattcgata aggtctgccc ccaccatgcc
    11701 tgcaaagacc agatcattcc tcaagaggtg gccgagctct gcttacatct cttgggtaaa
    11761 cgtccctttc catcgttttc gtccaagatt cgagtatatg aaggcggagt taatgaagat
    11821 cagttaggga cattcatgat aagagc
//
LOCUS       DATIAL010000139         3329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_883743, whole genome shotgun sequence.
ACCESSION   DATIAL010000139 DATIAL010000000
VERSION     DATIAL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883743"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2537
                     /locus_tag="VLA60_04415"
     CDS             <1..2537
                     /locus_tag="VLA60_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSF08633.1"
                     /translation="KSVSRESSGPQDSIHSVEWSTLEIDTIDIRYDDPSLKTRVRAQI
                     EENNSERLIVKAKGLYRNERIDMTVTAPAMKTFANLSPDSQSRKHLSAEFKLAGTTVS
                     LSAMVEPVWPPMWLDLTFSMSSESPDTLAAIFDGDLPALDTLALNGSLSKRTHLWSLQ
                     KFAAIIGESDISGQATIDTADELRFKGNLQSNTLNIASLMPANGKDTPTAQAVTSIPE
                     SSEQDTGQSHWPEKLLPPWLNNLQGVLGLQVEHLVLPGATLKDVTVQTTLDNGLLRIS
                     PMNIDLGGGTIKTTAQLNLRNPSWSGHLQTDIQEVDLNEAMQTLGREAGALGQISGRL
                     ALSLPAAHQTPKHLLNTDALLDQLRIDEVRLRYDDPALQAKTDLRLMADSVASGIQVT
                     GTVEYRENPVEVSITTGSIRQALQDYGAMPVDATFTTQETTLTLEGNVGELFPVAKFK
                     GTVGMKGPDPARLGEAIGIPLPHLPPYRLSAQVHREQQAESQQSFNLSDLDGTIGDSD
                     VAGKLRVTTGGERPMIFARLESRTLDFDDLAGLIGEPPDPDEAASKEQEAQAEAAEKR
                     KTLLPDKPIDFTQLRKLDADVEYRAKGVKALDLPLNDLVLNVLLKDGHMQMERLDFGV
                     ATGKVAMQLEVNAHESPAQAKLHTNFDHINLSQLLARFEVADDSFGDIGGRATLWMQG
                     ESLANWFASADGGLYLTMTGGKIDALLVELAGMDFSESAAVLLSTDTRVKIECAYTDL
                     QARSGIVTIHPFLLDTQDTKFKGHGSIDLRQEKLNLTVEPYPKDFTIMSSRGPLHVTG
                     TFSNPEFSVEPTFPSPEFGMADDSARCRGMVDALRNARKEQLADKN"
     gene            2732..>3329
                     /locus_tag="VLA60_04420"
     CDS             2732..>3329
                     /locus_tag="VLA60_04420"
                     /inference="COORDINATES: protein motif:HMM:NF037926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08634.1"
                     /translation="MSINTNDLQDLLEQKESPCVSLYMPTHPAGPEIRQDPIRLKNLL
                     KETEQQLTEQGIRSTDARTWLDPLSKLLEDEQFWRHQDQGLAMYVAKAWHRVFRLPLS
                     LPELTMVASRFYLKPILPIFMYGGTFYLLALSQNQIRFFQGSPDDLQELHIPDIPLSI
                     EETNQTAAGEQSLQWHTKAPNVGDQRAAMFHGQGGGDDE"
BASE COUNT          841 a          929 c          844 g          715 t
ORIGIN      
        1 agaagagcgt ttcacgggaa tcctccggcc cgcaagattc tattcactcc gttgaatgga
       61 gtacgctcga aatcgatact attgatatcc gatatgatga tccttccctg aaaacgcgcg
      121 tccgggcgca gatcgaggaa aataactcgg agcggttgat cgtcaaagcc aaaggtctgt
      181 atcgaaacga acgcattgac atgaccgtga cggctcctgc aatgaagacg tttgccaatc
      241 tctctcccga cagtcaatcc cggaaacatc tgtcggccga attcaaactg gcgggcacca
      301 cggtctcgct ttctgccatg gtcgaacctg tctggccgcc aatgtggctt gaccttacct
      361 tttccatgag cagtgagtct ccggatacgc ttgccgccat attcgatggt gacctccctg
      421 ctttagacac attggccctc aacggctcac tctccaaacg gacacacctc tggagtcttc
      481 aaaagtttgc cgccatcatc ggggagagcg acatttccgg acaagccacc atcgatacgg
      541 ctgacgaact gcgctttaag ggaaacctgc agtcgaacac cctgaatatt gcatcgctga
      601 tgccggccaa cgggaaggac accccgacgg cacaagccgt gacatcaatt cctgaatcct
      661 cggagcagga taccggccaa agccattggc cggagaagct tctcccaccg tggctgaaca
      721 atctccaagg cgtactcggc ctacaggtgg aacacctggt gctgcccggc gcgaccctca
      781 aggatgtgac ggttcaaacg actctcgaca atggactcct tcgcatttct cccatgaaca
      841 ttgaccttgg cggtggcact atcaaaacca ccgcacaact gaacctccgc aacccgtcat
      901 ggtcaggtca tcttcagacc gacatccagg aagttgatct caacgaagcc atgcagaccc
      961 taggccgcga ggccggagca ttgggtcaga tcagtggccg tctcgctctg agtcttcccg
     1021 cggcccatca gaccccgaaa catcttctca ataccgacgc attactcgat caattgcgca
     1081 tcgatgaagt gcgtctgcgt tacgacgatc cagcgttaca ggcaaagacg gatttgcgcc
     1141 tcatggcaga tagtgtcgca tccggaattc aggtcacggg cacggttgaa tatcgagaaa
     1201 accctgtaga ggtgtccatt actacgggtt ccatacgcca ggccttgcaa gactatggtg
     1261 caatgccggt ggatgcgact tttacgacac aagaaaccac ccttacgctt gaaggcaatg
     1321 ttggtgagtt gttccccgta gcgaaattta aggggacagt cgggatgaaa ggtcctgatc
     1381 ccgctcgtct gggcgaagct atcggcattc ctcttccgca ccttcccccg taccgattaa
     1441 gcgcacaagt tcatcgcgag caacaggctg agagtcaaca gagttttaac ttgagtgacc
     1501 tagacggcac catcggcgac agtgacgttg caggcaaatt acgtgtgacg accggcggag
     1561 agcgtccgat gatatttgcg cgattggagt cacgtacact cgacttcgat gacctggccg
     1621 gcctcatcgg cgaaccaccg gatccggacg aggcagcttc gaaggaacag gaagcgcaag
     1681 ccgaggcagc cgaaaagcgg aagaccctct tgccggacaa acctatcgac ttcactcaac
     1741 ttcgtaaatt ggacgcggat gtagaatacc gcgccaaggg ggtcaaggcc ctcgatctcc
     1801 ccctgaatga tttggtgctg aatgtgcttc ttaaggacgg acacatgcag atggagcgcc
     1861 tggattttgg cgtggccacg gggaaagtgg ccatgcaact tgaggtcaat gcccatgaat
     1921 caccagcaca agccaagctt cacactaatt tcgatcacat caatttaagt caattgcttg
     1981 cccgcttcga ggtggcggat gactcttttg gcgatatcgg aggacgtgcc acactttgga
     2041 tgcaaggaga atccttggcg aattggttcg cctcggcgga cgggggtctt tacctaacga
     2101 tgaccggagg aaaaatcgat gccctactcg tggagttggc cggtatggac ttcagcgaat
     2161 cagcggcggt actcctgagc acggatacgc gagtcaagat agaatgtgcc tataccgatt
     2221 tgcaggcccg cagtggtatc gtaaccatcc atcccttctt actggatacc caagacacca
     2281 aattcaaagg tcacgggtcg atcgatcttc gccaggaaaa attaaatctg accgtagagc
     2341 cgtatcccaa ggactttacc atcatgagtt cacggggccc cttgcacgtc accggcacat
     2401 tttcgaatcc ggaattttct gtcgaaccta cttttccctc ccctgagttt ggaatggctg
     2461 acgatagcgc ccgctgtagg ggcatggtgg acgcgctccg gaacgcccga aaggagcaac
     2521 tggcagacaa aaattgatcc tgcctttcgt atttaccacc tacaatctga aaggaaagag
     2581 tgctttccaa tcgtacccca tataactaag catcaggtga tggagagaaa agacttcagg
     2641 gaaattacca ggtttctacg caaattttcc gtgttaccct gaaaagtaaa tttgtgcagc
     2701 cgcccatctc tgaaccatcg aaagggtccc catgtcaatc aacacaaacg atctacagga
     2761 tctccttgaa cagaaagaaa gtccatgtgt gtccttatac atgccgaccc atcccgcagg
     2821 accggaaatc cgacaggacc cgattcgctt gaaaaacctt ctcaaagaga ctgaacagca
     2881 attgacggaa cagggcatca ggagcaccga cgctcgaacc tggctggacc ctctctcgaa
     2941 gctcctggag gatgagcaat tttggcggca tcaggatcag gggctggcca tgtacgtggc
     3001 gaaggcttgg caccgtgttt ttcgtctgcc cctcagcctt ccagaactca cgatggtggc
     3061 atcccggttt tatctgaagc ccatcctgcc catattcatg tatggaggaa cattttatct
     3121 tctcgcgctc agtcagaacc aaattaggtt ttttcagggt tcaccggatg acctgcaaga
     3181 attgcacata ccagacattc ccctcagtat agaggaaacc aatcaaaccg cagccggcga
     3241 gcaatcattg caatggcata ccaaagctcc caatgtaggc gaccagcggg ccgccatgtt
     3301 tcatggacag ggcggtggag atgacgaag
//
LOCUS       DATIAL010000140         7530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_561818, whole genome shotgun sequence.
ACCESSION   DATIAL010000140 DATIAL010000000
VERSION     DATIAL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561818"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VLA60_04425"
     CDS             <1..1186
                     /locus_tag="VLA60_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF08635.1"
                     /translation="QGSRSTFNNVSGMFFPNSFLQPISGPDLGRNSYSSSWGSAAGIL
                     LEWEPFDFGLRSAQVSTAQSMERHSQAFITLTQLEVALAVGDAYIDVMMAHESRQALQ
                     SNLDRRVVFAKTVDVLVRNQLRAGVDGSRAQAETAVAQTHLLEAEQVERSRMATLSQV
                     LGVAGTEISIKDPILGSSPQGSVSDKKDPAQHPLALVQKAAADIPKMREEALAHAWVP
                     KFNLQSAFFGRGSGWDNQGNRGGGGEGLLPDVPNWSVGLTATFSLLDSSTIRAQKQQE
                     HYRNLAEMARYDRVIQELTAQQVKARSLMESTKRIAENTPIQLQAARLTESQAAAQFK
                     AGLATVVDVAEAQRLVVQAEVDDARARLGVWKALLALSGVQGELSEFLRLARASSSSP
                     GH"
     gene            1186..4344
                     /locus_tag="VLA60_04430"
     CDS             1186..4344
                     /locus_tag="VLA60_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF08636.1"
                     /translation="MKLLLFALRRPLTIMVLVASMALFSYLVVKRMAIDIFPNLGLPA
                     IYVAQPYGGMDPAQMEAYLVSFYEYHFLYITGIEHVESKSIQGAALMKLFFHPDTDMD
                     QALAQTVAYVNRSRAFMPPGTVPPFISRFDAGSVPVGYLVFQSESRSLKDIQDLALFR
                     VRPMFSAVPGVSAPPPFGGTARTIVIKVNPERLRAFGMSPEEVVQAVRTGNMLMPAGN
                     VRIGELAYLTPVNSVVENITELEHLPIRLGAGPTVFLKDVGTVEDGEDIVTSYAMVNG
                     RRTVYIPVTKRADASTMAVVDRIKASLPTFRDAVPDDIQISFEFDQSTYVTNAVKAVA
                     VESALGAVLTGLVVWLFLRSWRSSVIVVMTIPFALLSAVVALWATGQTINIMTLGGLA
                     LAVGILVDESTVTIENVHAHLSRGKSLGRAVKDSRGEVVVPLLLAMLSILAVFLPSFF
                     MSGVAKALFVPLALAVGFAMAASYILSTTLVPVLAVWLLGGHPKAEGREGPTIPAWYV
                     NALSWTLSHKELVLIGYLLLSMAIVVGFGSRLGLDIFPRVDTGQFQLRILAPDGTRVE
                     RTEVLTKEILETIKKEIGPDRLNISLGFVGVHPSSYPVNTLYMWSSGPHEAVLLVALK
                     PGSDISLEQVKERLRELLPKRFPGTRYTFEGGDIVTQVMSMGSPTPIEITMSGPNLDA
                     NRAYAERVREELSHLPAIRDLRFGQPLDYPTLAVRVDRVRAGQLGVTAGEVARAVVSA
                     TSSTRFVEPIYWRDPESGRAYQVQLEIPPSEFQSVQDMLNLPVMLEGGRGPLLRDVAE
                     VIPGTSIGEYARYNMQRMVTIVANVTGEDLGRAAEHIEAALKRVGPPPKGVRVDVRGQ
                     IAPMKEMFVNLQLGLALALLAIFLLLAANFQSWRSALVVLLTMPAVLMGVVLGLALFG
                     ATLNIQSFLGAIMATGVAVANAILLVSFAEQYRKGGHSSFEAAREGACGRARPILMTG
                     LAMIVGMLPMAAGIMEGGEQVAPLGQAVIGGLVLATVSSLLILPAIYAILEKGGAISS
                     PSLDPDDPSSIHYEPSHVTVPK"
     gene            4357..5502
                     /locus_tag="VLA60_04435"
     CDS             4357..5502
                     /locus_tag="VLA60_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF08637.1"
                     /translation="MSGKMIGIGLLLLGLIGAGVMVLSNEKHVPRDPTVSSSSTHSLE
                     ISGIPAGSTQRVSVEVVPVSSQHLKETIPLPGELLPFLSVDLSPKIMGIVESVHVDRG
                     AKVKKGDILIQLRAPELQAQRREAEAQLRAANITYTRLQAAASTPGIVAGNDLELAQH
                     HQEALTARLQSLQEMEKYLRVVTPFDGVITQRNIHPGAVVGPDGGGGRASSLLRLEQI
                     VHLRLIVPVPEVYAGSIDKGATVPFSVPAYPEEIFQGVVSRISQSVDPKTRSMPVEMD
                     VDNPTMRLAAGMFPEVRWPIRRSTPTLFVPATAIVTTTENVFVLLVKENLVEWIPVRK
                     GAKSGFMVEVFGALQSGDLVVFRGTDEIRPGTKIFPVSKEIPLNPRT"
     gene            6490..>7530
                     /locus_tag="VLA60_04440"
     CDS             6490..>7530
                     /locus_tag="VLA60_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSF08638.1"
                     /translation="MNVLMMAGLGLGMAMLINSTSVLAAEPVSSSSSSLAVEKGSAYG
                     EFFTQIKDRAEAQNGKNWKETADASLIQSKETQWNRYLKAAMGLPDFVDLGIENRTRF
                     ESVDHPWRSTAAVGGGRTDSQWLLRSRLRFGLGNGPLRFLFEGQDSREYGAQIGGFVN
                     NTTVNEWDILQLFGSLTVDNIAGSELRTDLHFGRMTLDLGGRRYVARNDFRNTTNSFD
                     GFHWQIGKEKVWRFRTFLTEPVIREEVKLDEQNEKFLFWGAYVENLQIPWMNMEAYYY
                     GLNDQRQKNKNLHRTYGTYGMRLYKDPKVGEFDYELEAAVQVGHLGQVDHFAYNPNAQ
                     VGYTFNVPWTPQF"
BASE COUNT         1690 a         1732 c         2163 g         1945 t
ORIGIN      
        1 tcaaggaagt cgatcgacct ttaataatgt ctcggggatg tttttcccga attctttttt
       61 gcaaccgatc tccggtcctg atctgggacg taattcttac agcagtagct gggggagtgc
      121 cgcgggcatt ctgctggaat gggaaccctt tgacttcggg ttgagatcgg cacaggtgag
      181 taccgcgcag agcatggagc gtcattcgca agcctttatc acgctcaccc aactcgaagt
      241 ggctttggct gtgggggatg cgtatatcga tgttatgatg gctcatgaat cacgccaggc
      301 tcttcaatca aatttagatc gccgcgtggt cttcgccaag accgtggatg ttctggtacg
      361 caatcaattg cgggctggag ttgatggatc ccgtgcacaa gctgagacgg cggttgcgca
      421 aactcattta ttggaagcag aacaagttga acgttcacga atggcgacat tgtctcaagt
      481 gctgggagtt gcgggcacgg aaatttcaat taaggatccc attttgggaa gttcccctca
      541 aggatcagtt tccgacaaga aggatccagc acagcacccc ttagccttag tccaaaaagc
      601 cgcagccgat attccgaaaa tgagggaaga ggccttagcc catgcctggg tgccaaaatt
      661 caatttacaa agcgcctttt tcggccgtgg cagtggatgg gacaaccagg gcaatcgagg
      721 tggcggcggg gaagggctat tgccggatgt gccgaattgg tcggttggcc taacggcgac
      781 cttttctctg ttggattctt cgaccatccg cgcacaaaaa caacaagagc attatcgcaa
      841 tctggccgaa atggcacgct acgatcgagt gattcaagaa ctgaccgcgc agcaggtaaa
      901 agctcgatcg ctcatggaga gtaccaagcg cattgccgaa aacacgccca ttcaattaca
      961 agccgctcgc ctgacggaat cccaagcggc tgctcaattt aaagccggtt tggccaccgt
     1021 cgtggacgtg gctgaggcgc agcggcttgt ggttcaggca gaagtggatg atgcgcgggc
     1081 ccgtcttggt gtatggaagg ccttgttggc cttatcagga gttcagggcg agctgtcaga
     1141 attcttacgg cttgcgcgcg cgtcttcttc ttcacccggc cattaatgaa gctgcttctc
     1201 tttgcccttc ggcgacctct gaccattatg gtgctggtcg cgtccatggc cttattttca
     1261 tatctggtgg tcaaacggat ggccatcgac atcttcccga atctgggact tcctgccatt
     1321 tatgtcgccc aaccctatgg tggcatggat ccggctcaaa tggaggccta tctggtctcg
     1381 ttctatgaat atcatttcct ctatatcacc gggattgagc atgtcgaatc caaatccatc
     1441 cagggtgccg cattgatgaa attgtttttt catccggata cggatatgga tcaggctttg
     1501 gcgcagacag tcgcctatgt gaatcgatcc cgcgcattta tgcctcccgg tacggtgccg
     1561 ccttttattt cgagatttga tgctggaagc gtgccggtgg gctatttggt ttttcagagc
     1621 gagtcacgga gtctaaaaga cattcaagat ctggcgttgt tcagagtgcg tccgatgttt
     1681 tccgcggtcc ctggggtgtc tgcccctccg ccatttggag ggacagctcg aaccattgtg
     1741 attaaggtta atccggaacg gttgcgggcc tttggcatgt ccccggagga agtggttcag
     1801 gctgtgcgaa cgggtaatat gctcatgccg gcaggaaatg ttcgcatagg agagttggcc
     1861 tatttgaccc ctgtgaattc tgttgtcgaa aacatcacag aattggaaca tctgcctatt
     1921 cgattggggg ccgggcccac agtctttttg aaagacgtgg gaaccgtgga agatggagag
     1981 gacatagtca ccagttatgc catggtgaat gggcgacgca cggtctacat cccggtcacc
     2041 aaacgggccg atgcctccac catggctgta gtggatcgca ttaaagcctc cctcccgact
     2101 ttccgggacg cggtgccgga cgatattcag attagttttg aattcgatca atcgacctat
     2161 gttacgaatg cggtaaaggc tgtcgccgtg gaaagtgccc tgggagccgt cttgacggga
     2221 ttagtggtgt ggctatttct ccggagttgg cggagttcgg tgatagttgt gatgaccatt
     2281 ccatttgctc tcttatccgc cgtggtggct ttgtgggcaa cgggacagac catcaatatt
     2341 atgacgctag ggggcttggc cctagcagtg ggcattttgg tggatgaatc cacagtcacg
     2401 attgaaaatg tacatgccca tttgtctcgt gggaagagcc tggggagggc cgttaaggat
     2461 tcacgtggtg aagtggtggt gcctcttctc ctggccatgc tgagtatttt agcggtgttt
     2521 ctcccgtcat tttttatgag tggcgtggcg aaagcgttgt ttgtgccgtt ggcattggca
     2581 gtgggatttg ccatggcggc ctcctacatc ctttccacga cattagtacc ggttttggcc
     2641 gtgtggctgt tgggtgggca tcccaaggct gaggggagag aaggtccgac gataccggcc
     2701 tggtatgtca atgccttgag ctggaccttg agccataagg aactggtcct catcggctat
     2761 ttgctgctca gtatggcaat tgtcgttggt tttggttcca ggctggggtt ggatatcttt
     2821 cctcgggtcg ataccggcca atttcaatta cgaattctgg ctcctgatgg gacccgtgtc
     2881 gaacggacgg aagtgttgac gaaggaaatt ctggagacaa tcaaaaagga aatcggtccg
     2941 gaccggctta atatcagtct ggggttcgtg ggagttcacc cgtcaagtta cccggtgaat
     3001 accttgtata tgtggagcag tggtcctcac gaagctgtgt tgttggtggc gctgaaaccg
     3061 ggttccgata tttccttgga gcaggtcaaa gaacgattga gggaactgtt acctaagcgc
     3121 tttcccggga cgcggtatac ctttgaggga ggtgatattg tcacgcaggt catgagcatg
     3181 ggatccccca ctccgattga aatcacgatg agtggaccca acttggatgc caaccgggcg
     3241 tatgcggaac gagtgcgaga ggaactctcg catctacctg ccatacgcga tctgcgcttc
     3301 gggcaaccct tggattatcc caccctggca gtgcgcgtgg atcgagtgcg agcgggccag
     3361 cttggagtga cggccggtga agtggccagg gcggttgttt cggcaacctc ctcaacccgt
     3421 ttcgtggaac cgatttattg gcgtgatccg gaatctggaa gggcctatca ggttcaattg
     3481 gaaattcccc catcggaatt ccagtccgta caggatatgc tcaatcttcc ggtgatgctt
     3541 gaaggcggac gtgggccgtt gcttcgtgac gtagcggagg tcattcccgg aacctctatt
     3601 ggtgagtatg cacggtataa tatgcaacgg atggttacga ttgtggccaa tgtgacggga
     3661 gaggatttag gacgggcggc ggaacacatt gaagccgcgt tgaagcgtgt cggtccacct
     3721 ccgaaagggg ttcgggtgga tgttcgaggg caaattgctc cgatgaagga gatgttcgtc
     3781 aacctacaac tgggattggc cctggcattg ctggctattt ttctgctctt agccgcaaac
     3841 tttcaatcct ggcgaagcgc attggtggtc ttgctgacga tgcctgcggt attgatggga
     3901 gttgtgcttg ggctggcact tttcggggcc acgctgaata ttcagtcgtt cctgggggcc
     3961 attatggcca cgggggtggc tgtggcgaat gcgatccttt tggtgtcgtt tgctgaacaa
     4021 tatcggaaag gaggacactc atcattcgaa gctgcccgcg aaggggcatg cggccgggct
     4081 cgcccgatcc tcatgaccgg gttggccatg atcgtcggaa tgttgccgat ggcagcgggc
     4141 atcatggaag gtggagagca ggtagcccct ctgggacagg ccgtgattgg cgggttagtg
     4201 ctcgctaccg tgtctagctt actgatcttg cccgcaatat atgcgattct ggagaagggg
     4261 ggagccatct cttctccttc attagatccc gatgatccat caagtattca ctatgaaccc
     4321 tcgcacgtca ccgttcccaa atagctggga gttgctatgt caggcaagat gatcggaatc
     4381 gggctgttgt tgctgggact tattggggca ggtgtgatgg tcctgagcaa cgaaaagcat
     4441 gttcctcggg accccacggt ttcttcttcc tcaactcatt ccctggaaat atcaggaatt
     4501 cctgcgggga gtactcaacg ggtctcggtt gaagtggtgc ccgtttccag ccaacatctg
     4561 aaagagacga ttcccttgcc gggagaattg cttccttttt tgagtgtcga cctttccccc
     4621 aaaatcatgg gaatcgttga atccgtccac gtggatcgtg gggcaaaagt caaaaagggg
     4681 gatatcctca tccaattgcg cgcgccagag ttgcaagcgc agaggaggga agcggaagct
     4741 cagcttcgtg cggcaaatat cacatatacc cgtcttcaag ctgcggcctc tacgccgggt
     4801 atcgtggctg gaaatgattt ggaattggcc caacatcatc aagaagcact taccgcccgt
     4861 ctccagtcgt tacaagaaat ggaaaagtat ttgcgggtgg tgaccccgtt cgatggggtg
     4921 atcacacaac ggaatattca tccgggagcg gttgtggggc ctgacggcgg aggcggccgc
     4981 gcttcatcct tgttacggtt agagcaaatt gttcacttgc ggttgatcgt tccggttccg
     5041 gaagtctatg ccgggtcgat tgataaaggg gcaaccgtgc catttagcgt gccggcctat
     5101 ccggaggaaa tttttcaggg agtggtgtct cgtatttctc aatcggtaga tcctaaaacc
     5161 cgatccatgc ccgtggaaat ggacgtcgac aatccgacta tgcggttggc tgcagggatg
     5221 ttcccggagg ttcgctggcc gattcgccgg tccactccca cattgttcgt tccggccacg
     5281 gcaattgtca ccacgacgga aaacgttttt gtgctgttgg tcaaagagaa cctggtggaa
     5341 tggatccccg ttcgaaaggg agcaaagagt ggcttcatgg ttgaagtgtt tggcgctctc
     5401 caatcgggtg atctcgttgt gtttagggga actgacgaaa tcaggccagg tacaaaaatt
     5461 tttccggttt ctaaggagat acccctcaac ccccgtacct agatcagctt ccttcgagaa
     5521 agccccttcg attaacggtg aaaattcttc tgctcataca ccgaattttt tatggtgtgt
     5581 cattcaaggg ctattcatgc agacatccat tcggcgattc aaggcaaggt gcttttgagg
     5641 ggctaccttg tgtcgaatgc tgaatcaaaa aaatcgtgct gtacttccgg tggattccaa
     5701 aggaaaagca tgacattcta acggccgacg ggcaaagaca ggctttagca ttagggcatt
     5761 ttcttcatga gaaatgcatt gtcggcattc taagttctca tgcgggtcga acgtgtgaga
     5821 tggcaaaagc tatcgggcag tgcatgggct tgaaggcagg atatgaggtg gatcaggcta
     5881 ttgcggcatg gcgtgggggg ggaagcctgg atgggtgcca gatttcatgg tgttggcgtg
     5941 agaagcagtg gctttaggac agggttcacg ccctgaagga tgagagtctc tcgcagatgg
     6001 aaaatttcat gctaaggctg gctttaaaaa gcaggctgaa aaatccccgg aaaagccggt
     6061 cgtaattgtc tcgcacggtg ataggtctaa tgcgttgctg ggggtatgca gcgaatatcc
     6121 cgattcccaa acgagatgtg tcactcagga tgtcccaacc ggatctgtca acgaactcat
     6181 cctgacaaat acaggctggc acatcatcga agaagggggg caatcattcg aataggtctc
     6241 ctttgctttc ccaagtaacg gactctcttt attttttctc ccgatcctaa aattcctcga
     6301 gatgtgttcc cctgagtaac aatattccac cagccaggag gactttttgg tttcccggaa
     6361 tgatgtatct ttaacttgac ataaatacat tttaaaatta cattaaaccc aaaatttttt
     6421 ggggaaacat tttttgatca gttaaacgtc caaggaagaa tgtgtcgtaa tgagaaaaag
     6481 gcaagtaatg tgaatgtctt gatgatggct ggattgggat tgggtatggc catgctgatt
     6541 aattccactt cggttttggc ggccgagccg gtttcttcga gttcctcttc cttagccgtt
     6601 gaaaagggtt cggcttatgg agagtttttt acacagatta aggatcgggc agaagcacag
     6661 aacggaaaaa attggaagga aacggctgat gcttcgctca tccagtcaaa agagactcaa
     6721 tggaatcgtt atctgaaggc ggcgatgggt ctgccggatt tcgttgactt ggggattgaa
     6781 aaccgcacgc gcttcgaaag tgttgatcat ccctggcgat ccactgcagc agttggaggc
     6841 gggcgaacgg attcgcagtg gctcctcagg tccaggttgc gcttcggatt gggtaatggc
     6901 cctctccggt ttctctttga gggacaggat tctcgcgaat acggggccca gattgggggt
     6961 tttgtcaata atacaacagt taatgaatgg gatattttac agttattcgg atcattaacc
     7021 gtagataata ttgcaggaag tgaattgcga accgacctgc atttcggtcg tatgactctg
     7081 gacctgggag gtcggcggta cgtcgcccgt aatgatttcc gaaataccac gaattccttc
     7141 gacggctttc actggcagat tgggaaagag aaggtttggc gattccggac ctttctcacc
     7201 gaaccggtta ttcgtgagga ggtcaaactc gatgagcaga atgaaaagtt tttattttgg
     7261 ggggcgtatg tggaaaacct tcagatcccc tggatgaata tggaggccta ttattacggt
     7321 ttaaacgatc aacgacaaaa aaataaaaat cttcatcgta cgtatgggac gtatgggatg
     7381 cgcctctaca aggatcctaa agttggtgaa ttcgattatg aattggaagc ggccgttcaa
     7441 gttgggcatt taggacaggt cgaccatttt gcctataatc ccaatgcgca agttggctat
     7501 acgtttaacg tgccctggac tcctcaattt
//
LOCUS       DATIAL010000141         9943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1207628, whole genome shotgun sequence.
ACCESSION   DATIAL010000141 DATIAL010000000
VERSION     DATIAL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207628"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VLA60_04445"
     CDS             <1..500
                     /locus_tag="VLA60_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HSF08639.1"
                     /translation="IVVSSVEGSRPLLVELQALVAETTYPMPKRMAKGVEVNRVSLLL
                     AVLEKRLGLHFTGYDVYVNVVGGLRIEEPTVDVGIVCAVLSSFRELALDSRLVVMGEV
                     GLGGEVRPVQHADLRIREAMKLGFQRCVVPEPNLHQWKPVAGMEVVGIRDIGDIWETV
                     VSPAS"
     gene            528..1313
                     /locus_tag="VLA60_04450"
     CDS             528..1313
                     /locus_tag="VLA60_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08640.1"
                     /translation="MNGVSRWFLLPVVFLVGCAGMFQGSLHLLEDERDRQEVFQALRQ
                     KESTIRTLRGLFQASISGSGVPLSQNVLGTVSYVHPDVLDLKTFIRMGVPVMNFHRKG
                     NEYELNLPGEDKVITGRLDEPQERSRWDHTVMLSLRALDAMLGKVSRGVGEEARVWKN
                     NEHYRIDLAANPSSSTSSQDDFTVRTWVDAQTMDLTSIEYRRSFDDIVVFVECEDYRE
                     VKVTTGEESAPVRLPFLVRATDYRPSGGSITLHFKEFIVNAAS"
     gene            1363..2058
                     /gene="tsaB"
                     /locus_tag="VLA60_04455"
     CDS             1363..2058
                     /gene="tsaB"
                     /locus_tag="VLA60_04455"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HSF08641.1"
                     /translation="MLFLALESATSHQSVAVFRDQQLLASRACESGQPLTPQLIPTID
                     RLLFSVSLQLSNLEGLVVSIGPGTFTGLRVGLATMTAFRLALQIPLVGVSTLEGLAWN
                     HPVTELPLLSTIGIRQGIVYWALFRWENHQMVCVKESQIGDIIEVCGALTEPTIVLGD
                     GWMQNRATLLSKGFSLIEAPPDYQWPSAKGIGMAGRVLLERGALLPLGCTPHYIQPSY
                     AEISKTKSLNPIG"
     gene            2106..2660
                     /gene="rimI"
                     /locus_tag="VLA60_04460"
     CDS             2106..2660
                     /gene="rimI"
                     /locus_tag="VLA60_04460"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HSF08642.1"
                     /translation="MDTNDPVAGSEVSCNEGIRLATLADLDALVSLEESCFSVPWSKK
                     SFEAELHGNAFSQILVIPGPKEQQEIPLLAYSCVWVIFEEIRFLNLAVHPHFRGQGLA
                     KQLILQALCIGSARGCCRGMLEVRNSNQVAKRLYRGFEFKEYGRRSSYYTNPSEDAIL
                     MFLEPLFKPKSDKNDLQGIPAECK"
     gene            2708..2953
                     /locus_tag="VLA60_04465"
     CDS             2708..2953
                     /locus_tag="VLA60_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HSF08643.1"
                     /translation="MVTDEQIAENLRASNVEYQELEESHHRLDLELQQLLKHHVLTPQ
                     EEILKKHLQKEKLGKKDRMAALIREHRASCDNAKTPG"
     gene            2992..3792
                     /gene="tatC"
                     /locus_tag="VLA60_04470"
     CDS             2992..3792
                     /gene="tatC"
                     /locus_tag="VLA60_04470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSF08644.1"
                     /translation="MAAFSPTMAMNAVVHPLQRHIRDIKRRLLIVGATIMVFFVIAFS
                     YSSILIDWFKRPFEDDLIFYAPAEALFASIKISFMAAVIASVPIILYQFWKFIEPALL
                     QKEQRWAVPLFFLGLGFFLLGLGFCNMVILPLVINFFVTFGMDRAITPELAVGTYVDF
                     NVKFLLAFGFAFEIPLVLSLLARVGVIQASVLIRFRKHAALVALILSAVITPDATMFT
                     MLLMAVPLIILYEIGIWGAKVFGRAPLPAGEKCEATEEGEASDHAAKG"
     gene            3851..4918
                     /locus_tag="VLA60_04475"
     CDS             3851..4918
                     /locus_tag="VLA60_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08645.1"
                     /translation="MGTVASLWLMGFSGIGENHSLISSLYSVSNIPSLHPDDSQKTQK
                     GFSPSIQTLVVTPDGVVYAGSFGMGLFRSQDKGKSWEALNRGLTDPFLLCLAVIPGKH
                     IYAGTMRGGIYRLELKGTTWESIGTGLHESEVKSFLVHQDVVYAGTGTGVYRWVEAEK
                     QWVTVGAGLDRILVPGLAIMDDGKLLAATSGKGLFHLETQHSSPSTWVDSQSIFVDPR
                     ERLPHRYLRVIAVNEAQHIFLGTQDGGIFTSTDRGQSWSSLSRNLPNDSIRSIVPDHK
                     DVIVATGNGIFRWKTDQQKWMGMNTGLTNLAIQSLTISNTGELYAGTSSGAFRSQDGG
                     AHWTNISQGLGVQLVPKGPYE"
     gene            4945..5466
                     /locus_tag="VLA60_04480"
     CDS             4945..5466
                     /locus_tag="VLA60_04480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSF08646.1"
                     /translation="MSDATHECQIITIHTQVNGEAWGDIHLRLFPDVAPNHVQNMVKL
                     AKEKFYDGTTFHRVIPNFMIQGGDPNSKEHDRSRHGMGGPGHRVNAEFSSKPHKRGTL
                     SMARSQDPNSAGSQFFICVADSSFLDGQYTVFGEVVSGMETVDRIVSAKRDANDNPLD
                     RIEMTMSVSDANA"
     gene            5645..6139
                     /locus_tag="VLA60_04485"
     CDS             5645..6139
                     /locus_tag="VLA60_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08647.1"
                     /translation="MSRRQMIEALSGLRVREYRSGPIRWIRGLADPEPAFNKGEIELL
                     VPRLLEGLGLAVPQELVTFYISQPVNATKREVTSGGMYVRDGYLHVIISNHRTSYEIP
                     PAGLIYDRRYPLFSLAPLDVDLLYETEETVLPKKKNFWDAIFGDEHSGEIVLDLSRLS
                     MMKM"
     gene            6311..7510
                     /locus_tag="VLA60_04490"
     CDS             6311..7510
                     /locus_tag="VLA60_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HSF08648.1"
                     /translation="MKKMVSKIPKISGEAKVSLQESVCNRLRDRRKKQGISQAELAVL
                     VGLTRQAVYAIEANQYLPSTHIALRFARALKCRVEDLFILADHEEIVEAEFIGGTTPL
                     GQPTRVKLSYVGSRILARPMAELGDVLNFAMPADGVIQEQAKKSSSGKRPSVRVQLLN
                     SSDVIKNGILIAGCDPALFLAGEHVRKINTLAGITNWTMGSANALRALQRDEVHMAGL
                     HLVDVKSGQSNVPYLKRHIPGQDFVGVRFASWVQGLLVQPGNPKHIGGVDDFGQSGLR
                     LVNRELGAGARFLLDSLLQKSGLTGNQLLGYGHEVPSHLEVARLIRDGMADVGIGVEA
                     AARHYGLGFIPLREEQYDLIMRREFLKSHPVMSQFLDTMVSQPFRREIESLGGYTVTE
                     IGKILHW"
     gene            complement(7683..8033)
                     /locus_tag="VLA60_04495"
     CDS             complement(7683..8033)
                     /locus_tag="VLA60_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08649.1"
                     /translation="MLIPHHLLGFLLGLIVFFQPGSQVFAEASTSLQKFNSPIMKIES
                     DKGVFLITTDSGIQWVQVEEAAKIQLKTLDVGDIIDVIVEMRPDNTPPLVKSWKLARS
                     GSSCKVFNGKTCSP"
     gene            complement(8145..8654)
                     /locus_tag="VLA60_04500"
     CDS             complement(8145..8654)
                     /locus_tag="VLA60_04500"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HSF08650.1"
                     /translation="MLTIPSSIINNMIVHARELAPHECCGILSGTENTIAEDYRITNI
                     LAALTEQDLTRFDAAKLTDLQRLSPEERADIAFQMDAREMAMAQRDIRSKKLDLLGFY
                     HSHTFSPARPSQTDITIAMEFESYRAKLHLPEPFHLIISLEHTDHPVVRAYKIQESKA
                     TEVPIHTLP"
     gene            complement(8659..9459)
                     /gene="moeB"
                     /locus_tag="VLA60_04505"
     CDS             complement(8659..9459)
                     /gene="moeB"
                     /locus_tag="VLA60_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HSF08651.1"
                     /translation="MEFTESQIQRYSRQIILSEVGGKGQKKLQDAKILVIGAGGLGSP
                     AALYLAAAGIGTLGLTDGDVVDLSNLQRQILHTTDRIGVSKVESGGTLLSALNPETTL
                     KLYPEHVSVTNILSLIEGYDIILDGSDNFSTRFLVNDACFFAKRTLISGSIFRFEGQL
                     ATIKPHEGFPCYRCLYPEPPPAGLVPNCQEAGVLGVLAGTIGVLQANEAIKEVLGLGE
                     SLAKHLLLYDALDMTFRKVGRPKSPDCPLCGPHPTITKLVEQEVSCSI"
     gene            complement(9474..9716)
                     /locus_tag="VLA60_04510"
     CDS             complement(9474..9716)
                     /locus_tag="VLA60_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HSF08652.1"
                     /translation="MMAKLRFHIRYPEDKIPSPILYEIGQEYQVVPSIRRADVRETTG
                     WMDVEFSGETDEIDRAIQGLRQKGCMVDPIELNVVE"
     gene            complement(9713..>9943)
                     /locus_tag="VLA60_04515"
     CDS             complement(9713..>9943)
                     /locus_tag="VLA60_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HSF08653.1"
                     /translation="GKSEVEITGNTVSEIIDNLGSAHPGIKERVYDEQGEVRRFINIY
                     VNEEDIRFLTGKDTPLKDGDEVSIIPAIAGGS"
BASE COUNT         2376 a         2231 c         2672 g         2664 t
ORIGIN      
        1 cgattgttgt gtcgagcgtg gaagggtctc gtcccttatt ggttgagttg caggctttgg
       61 ttgccgagac cacctacccc atgccgaaac gtatggccaa aggtgtggaa gtgaatcgag
      121 tgtccttgct gttggccgtc ctagaaaaac gattagggtt gcactttacc gggtacgatg
      181 tgtacgtgaa cgtggtggga gggcttcgga ttgaggagcc gacagttgac gtgggcatcg
      241 tatgtgcggt gctttccagc tttcgggagt tagcgttgga ttccaggttg gtggtgatgg
      301 gcgaggtcgg gttgggtgga gaggtccggc cagtgcaaca tgcagatcta cgcattcgtg
      361 aggcgatgaa attggggttt caacgctgcg tggtgcctga gccgaatctt caccaatgga
      421 aacctgtggc tggcatggaa gtcgttggca ttcgtgacat tggcgatatt tgggaaacgg
      481 ttgtgagtcc tgcctcataa acacagtgga ccatcggata tgttgcaatg aatggtgtca
      541 gtcgctggtt cttgttgccg gtggtgttcc ttgtcggatg cgccggaatg tttcaaggat
      601 cattacacct gcttgaagac gagcgggacc gacaggaggt tttccaagcc ctacgtcaaa
      661 aagagtcgac cattcgaact cttcgaggat tatttcaggc gtccatctcc ggttctggag
      721 tgccgctttc ccaaaatgtt ctgggcacgg tttcgtatgt gcatcccgat gtcttggatc
      781 tgaaaacctt tatccgtatg ggtgttcccg tgatgaattt tcatcgcaag ggtaacgagt
      841 atgaactgaa tttacccggt gaagacaagg tgatcaccgg aaggctagac gaacctcagg
      901 agcgttcacg ctgggatcac accgtgatgc tgagccttcg tgctttggat gccatgttgg
      961 gtaaagtctc gaggggagtc ggtgaggagg ctcgcgtgtg gaaaaataac gaacactatc
     1021 gcattgatct ggctgccaac ccatcctcat ctacctcatc ccaggatgat ttcaccgtac
     1081 ggacctgggt ggatgcccag actatggatt tgacgtcgat tgaataccga cgctcgttcg
     1141 atgacattgt tgtatttgtc gagtgtgaag attatcgaga ggtgaaagtc actacgggtg
     1201 aagaaagcgc tccggttcga ttgccgtttc tggttcgggc gacagactat cgacccagcg
     1261 gaggatccat tactctgcac ttcaaggagt tcatcgttaa tgcggcctcg tagttgccct
     1321 tagcacaagc aatcactaat cggtaagtta ggacccatca ttatgttgtt tttagcttta
     1381 gaatctgcca cctcccatca gagtgtggcc gtatttcggg accaacagct gttagccagt
     1441 cgggcctgtg agtctgggca gcctttgact ccgcaactta tccccaccat cgatcggctg
     1501 ttattctcgg tctctttgca gttatcaaat cttgaaggct tggtggtgtc cataggaccg
     1561 gggacgttca cgggattacg ggtggggttg gcaaccatga cggcctttcg gctggcttta
     1621 cagattcctt tggtgggggt ttccacactc gaagggcttg cctggaatca tcctgttacc
     1681 gaactccctc tgctcagcac tattggtatt cggcagggga tcgtgtattg ggcactgttc
     1741 cgttgggaga atcaccagat ggtgtgcgtg aaagagtcac aaattgggga catcattgag
     1801 gtctgtggcg ctctgaccga gccgaccatt gtgctcggag atgggtggat gcagaaccgg
     1861 gcgactctcc tttcaaaagg gttctccctt atcgaagccc ctccggatta tcaatggcca
     1921 tcagccaaag ggattggaat ggcaggacgg gtcctgctgg aacgaggggc tctccttccg
     1981 ctaggctgca ccccacatta tattcagcca tcatatgcag aaatatcgaa aacgaaaagc
     2041 ttgaatccca tcgggtgaga cttttggggt acctgttcgt gtgacatcaa cagaggagtg
     2101 gaagcatgga cacaaatgac cctgtcgcgg gaagtgaggt atcatgcaat gagggtattc
     2161 gattggccac attggctgat ttggacgcat tggtgagtct ggaagagtcc tgcttttctg
     2221 tcccctggtc gaaaaaaagt tttgaagccg aattgcacgg gaatgccttt agccaaattc
     2281 ttgtcattcc aggcccaaag gaacaacagg aaattccctt gctggcctat agctgcgtgt
     2341 gggtgatctt tgaagaaatc cgctttttaa atctggcggt tcaccctcat tttcgtggac
     2401 agggattggc taagcagtta attctgcaag ctttatgcat cgggagtgct cggggatgtt
     2461 gtcggggaat gttggaggtt cggaactcca atcaggtggc aaaacgtctc tataggggct
     2521 ttgaatttaa ggaatatggc agaagaagtt cctactatac gaatccaagt gaggatgcta
     2581 tccttatgtt tttggagcct cttttcaagc caaaatctga caaaaatgat ctccagggaa
     2641 tcccggccga atgtaaataa gaaacgctca ccagttcaac gttcactact aacacaggag
     2701 ggcaccgatg gtgacggacg aacaaatcgc cgaaaatttg cgggcatcca atgtggagta
     2761 tcaggagttg gaggaatccc atcaccggtt ggatctggag ctccagcagt tattgaaaca
     2821 ccatgtattg actcctcagg aagaaatact taaaaaacat ttacaaaagg aaaaacttgg
     2881 caagaaagat cgaatggccg ctctgattcg tgaacatcgt gcctcatgtg ataatgccaa
     2941 aacacctgga taatccgaac ggctgcttcg cactttaagt cccactttct aatggctgct
     3001 ttttccccta ccatggccat gaatgcggtg gtgcatcccc ttcagcgcca cattcgtgat
     3061 attaaacgcc ggttactgat cgtgggagcc acgatcatgg tgttttttgt tatagcgttt
     3121 tcttattctt ccattctcat cgactggttc aaacggccct ttgaagatga tctgattttt
     3181 tacgctccgg cagaggcatt atttgcctcc ataaaaatct cttttatggc ggcggtgatt
     3241 gccagtgtcc ctatcattct gtaccaattt tggaagttta ttgagccggc gttgctgcag
     3301 aaggagcagc gatgggccgt gccgcttttc ttcctcgggt tggggttttt ccttctgggg
     3361 ttgggctttt gcaatatggt gatcctgccg ttggtcatca acttttttgt cacgtttggc
     3421 atggatcgag ccattactcc agaattagct gttggcacct atgtcgattt taacgtcaaa
     3481 tttcttctgg cgtttggctt tgcgtttgaa attccactgg tgctttctct tttggcccgt
     3541 gtgggtgtca ttcaagcatc tgtccttatt cggttccgca agcatgccgc tctggtggcg
     3601 ttaattctct ctgcggtgat caccccggat gcaacaatgt tcaccatgtt attaatggct
     3661 gttcccttga tcatactgta tgaaataggc atttggggtg caaaggtctt tgggcgtgct
     3721 cctctgccgg cgggggagaa gtgtgaagcg actgaggagg gagaggcatc cgaccatgca
     3781 gcaaaaggtt aagcggattg gacccgtcca cccatcgacg cgaatgccaa gacctctatg
     3841 gaattgtctt ttgggcacag ttgcctcgct ctggcttatg ggattttccg gaattggaga
     3901 aaatcattct ctgatttcgt cgctgtattc cgtctcgaat attccctctc ttcatccaga
     3961 tgactcccaa aaaacccaaa aaggattttc ccccagtatt caaaccctcg tggtgacgcc
     4021 agacggagtg gtctatgcag ggtccttcgg aatggggctt tttcgaagcc aggataaggg
     4081 gaaatcctgg gaggcgttga atcgaggttt gactgaccct ttcttgctgt gtctggctgt
     4141 gattcccggg aagcatatct atgccgggac aatgcgtggt ggaatttatc gtcttgagct
     4201 caagggtacg acgtgggagt cgattggaac cggattacat gagtcggaag tcaaatcatt
     4261 tttggttcac caggatgtcg tgtatgcggg aacaggaacc ggggtgtatc ggtgggttga
     4321 agccgagaaa cagtgggtca cagttggagc cggattggat cggattctgg ttccgggcct
     4381 tgcgatcatg gatgatggca agctgttggc ggcgacttca gggaaaggtc tctttcatct
     4441 ggagactcaa cactcctctc cttctacatg ggtggactcc caatccatat ttgtcgatcc
     4501 acgggagcgg ttgccgcatc gctatcttcg agtcattgcc gtgaatgagg cgcaacacat
     4561 ttttttaggg acacaggatg gggggatttt caccagtacg gatcgtggcc aatcctggtc
     4621 ctccctcagt cggaatcttc cgaatgattc aatcaggagc attgtccccg atcataaaga
     4681 tgtcattgtg gcgaccggaa acggaatttt tcgatggaaa actgaccagc aaaaatggat
     4741 gggcatgaat accgggttaa ccaacctggc tatccaaagt ctcacgatat cgaataccgg
     4801 ggagttatat gccggaacca gtagcggagc atttcggagc caagacgggg gtgcgcattg
     4861 gacgaatatt agccaggggt tgggtgtcca gttggttccc aagggccctt atgaataaat
     4921 cggtggaacg gaaaggatgg gataatgagc gacgcaactc atgaatgtca gataatcact
     4981 attcacaccc aggtgaacgg tgaggcctgg ggggatatcc acttaaggct ttttccggat
     5041 gtcgcgccta atcatgtcca gaatatggtg aaattagcca aggagaagtt ttatgacggc
     5101 acgacatttc atcgggtgat tcccaacttt atgattcaag ggggagatcc caacagtaag
     5161 gagcatgatc ggtcacgcca tggaatgggt ggtccaggtc atcgggtcaa tgcggaattt
     5221 agctccaagc ctcataaacg cgggacgcta tcgatggcga gatcacaaga tcccaatagc
     5281 gcgggctccc agttttttat ttgtgtggcg gattcgagtt ttttagatgg tcaatatacg
     5341 gtgtttggtg aagtggtgag tggtatggag accgttgatc gaatcgtcag tgccaagcgg
     5401 gatgccaatg ataatcctct tgaccgaatt gaaatgacca tgagtgtttc tgacgcgaat
     5461 gcctaagaag tggcggcgcc tctggccttg ttgtctactt tttggatggc tattcgcggg
     5521 ctgtgtcgct cctccattct attcctacct ggtctatgaa aatcctacat cgttcgttcg
     5581 acttgaggtc tccccctggg tcgaccctga tgttcctcag acattgaacg agcatcccgc
     5641 caccatgtct cgtcgccaga tgatcgaggc cctgagcgga ttacgggtgc gtgagtatcg
     5701 ttccggcccc attcgctgga ttcgtggcct cgcggatcct gagccggcat tcaataaagg
     5761 cgaaatcgaa ttattggtgc cacgcctgct ggagggatta ggattagcgg ttccacagga
     5821 acttgtcacg ttttacatca gtcagccggt gaatgctacg aagcgggaag tgacctctgg
     5881 agggatgtat gtcagagatg ggtatctcca cgtcatcatc agtaatcacc gtacaagcta
     5941 tgagattccc cctgcagggc tgatctatga tcggcggtat ccgctgtttt ctcttgctcc
     6001 acttgacgta gatttactct atgagacaga agagacggtg ctgccgaaaa agaaaaattt
     6061 ttgggacgct atttttggag acgaacatag cggtgagatt gttctcgatc tttcccgatt
     6121 gtcaatgatg aaaatgtaag gataagttcc taagttgacc ggcatgggat ggaagggggc
     6181 gggaatgaca tctacgagtc cttgagcaat gaggttacta ttgagaaact taaggatgat
     6241 taatatgttt ttagcagatt atggagacca gcgcccatcc tccgtgatat tccgttagaa
     6301 taatacgatt atgaaaaaaa tggtttctaa aattccaaaa atttcagggg aggcgaaggt
     6361 ctcgcttcaa gaatccgtgt gtaatcgctt acgggataga cggaaaaaac agggaatctc
     6421 acaggcggaa ttagcggtcc ttgttggtct gacacgacaa gccgtatatg ccatagaagc
     6481 caatcaatat ttaccgagca cccacattgc cctacggttc gctcgtgcgt tgaagtgccg
     6541 agttgaggat ctttttatcc ttgcagatca tgaagaaatt gttgaagctg agttcattgg
     6601 tgggacaacg ccactgggtc aaccgacaag ggtgaagctg tcatacgttg gatcacggat
     6661 actagccagg ccgatggcgg aactgggaga tgtgttgaat tttgcgatgc cggctgatgg
     6721 agtcattcag gaacaggcga agaagtcttc cagcggaaaa aggccttctg tgcgggttca
     6781 attactgaat tcttcggacg tgatcaaaaa tgggattctt attgcagggt gtgacccggc
     6841 gttatttctc gccggggagc atgtccgcaa aattaatacc ctggcgggaa ttacgaattg
     6901 gaccatggga agcgcgaatg cgctccgtgc gcttcaacgg gatgaagtgc acatggcggg
     6961 attgcatctt gtggatgtga aatccgggca aagtaacgtt ccttatctga aacggcatat
     7021 tcctggtcag gattttgtcg gagtgcgctt tgcttcctgg gttcaaggtc tgttggttca
     7081 gccgggaaac cccaaacaca ttggtggggt ggatgacttt ggccagtcgg gacttcgtct
     7141 cgtcaatcgg gaattgggag ccggagcgag atttcttttg gactccctgc ttcaaaaatc
     7201 agggttaact ggaaaccagt tgctgggata tggccacgaa gtgccgtctc atctcgaagt
     7261 cgctcgcttg attcgagatg gtatggccga cgtgggcatt ggagtggaag ccgcagctcg
     7321 tcattatggt cttggcttca ttccgcttcg tgaagaacag tatgacttga ttatgcgcag
     7381 agaatttctt aaatcccacc ccgtcatgtc tcaatttctt gatacgatgg tcagtcaacc
     7441 attcaggcgg gaaatcgaat cactgggggg atacacggtg acggaaatcg gaaaaattct
     7501 tcattggtaa atctgcctct ctccctgcat ctccggcatt tccggagtca tggccaatgg
     7561 acatatgttc tacttgcgta aagctgccag gaatgtatcg gctgggttat ggcccattga
     7621 ggaaatggac atcagctgaa gttggtgaaa agtagcgggt agattgtgcc gttgaaaaag
     7681 tcttaaggac tgcaggtctt gccattgaat actttacagg acgaaccact tctggctaat
     7741 ttccatgatt taaccaatgg cggagtatta tccggtcgca tttcgacgat cacgtcgatg
     7801 atatctccaa catccaatgt tttcagttga atttttgccg cttcttcaac ttgaacccat
     7861 tgaatgccag aatcggtcgt aattaaaaat acccctttgt ccgattcaat tttcataatg
     7921 gggctgttga acttctggag cgaggtcgaa gcttcggcaa acacctgtga tccaggctga
     7981 aaaaatacaa tcaaccctag cagaaaacct aacaggtgat gggggattag cataaatttc
     8041 tcctcttgtt gctttgagaa gcctttctca ttatctcaag attcctggtt agaagtctat
     8101 ggtctttcca ataaaaatta caggcacggg tgggaggccc tgacttaggg taaggtatgg
     8161 atgggaactt ctgtggcttt ggattcctgg attttatagg cccggactac agggtgatcc
     8221 gtatgctcca aggaaataat caagtgaaat ggttccggca ggtggagttt ggcgcggtag
     8281 ctttcaaatt ccatggcgat ggtgatatcc gtttgagagg gtctagccgg actaaaggtg
     8341 tgggaatggt aaaaacctaa gagatcaagt ttttttgaac gaatgtcccg ctgagccatg
     8401 gccatttctc tggcatccat ctgaaaggcg atatcggctc gctcctcagg cgataagcgt
     8461 tgcaggtcag tcaatttggc tgcgtcaaag cgtgtaagat cctgttctgt taaggcagcc
     8521 agaatattgg taatgcgata gtcctcggcg atggtatttt cggttcctga gagaatgccg
     8581 caacattcat gaggagctaa ctcccgggca tgaacaatca tattgttgat gatgctggag
     8641 ggaatggtaa gcatggacct agatgctaca ggagacttcc tgttcaacaa gtttggtaat
     8701 agtgggatga ggtccacaaa gaggacagtc tggagatttt gggcgcccaa cctttctgaa
     8761 tgtcatatcc agggcatcgt aaagaagaag atgtttggcc aaactttctc caagaccaag
     8821 gacttcttta atggcttcat tagcctgcaa aacaccaatg gtcccggcca acacgcctaa
     8881 caccccagcc tcttgacaat ttggcacaag gccggcaggt ggaggctctg gatataaaca
     8941 gcgataacag ggaaaacctt catggggctt gattgtggcc aattgtcctt caaagcgaaa
     9001 aatgctccct gaaatcagtg tccttttcgc aaaaaaacag gcatcattga cgagaaaccg
     9061 tgtggaaaaa ttgtctgatc cgtccaatat gatgtcatag ccttcaatta aggagaggat
     9121 attggtgacg cttacgtgtt cgggatagag cttaagcgtg gtttcaggat tgagggctga
     9181 caataatgtc ccgccggatt caacctttga aacacctatc cggtcggttg tatgcagaat
     9241 ttgccgttgc agattggata agtccacgac atcgccatca gtcagaccta aggttcctat
     9301 cccggccgca gccaaataca gagctgccgg agaaccaagc cctcctgctc caatcaccag
     9361 gattttggca tcctgtaact ttttctgtcc tttgccgccc acttccgaca gaatgatttg
     9421 acggctgtac cgctggattt gagattcagt aaattccatg agaagcgaaa tccttattca
     9481 acgacgttca gttcaatcgg atcaaccatg cagccttttt gacgaagacc ctgaatagcc
     9541 cggtcaattt catcagtttc tccagaaaat tccacatcca tccatccggt cgtctcacgg
     9601 acatcggccc gtcgaatact gggcacgacc tggtattcct gcccgatttc atacaggatg
     9661 ggagaaggaa ttttatcttc gggatagcga atgtgaaagc gtagtttggc catcatgaac
     9721 ctcctgcgat tgcggggatg atggagactt catctccgtc cttcagtggg gtatccttgc
     9781 cggtcaaaaa ccgaatatct tcctcgttga cgtagatatt gatgaaccgc cgtacctctc
     9841 cctgttcatc atagactcgt tccttaatgc cgggatgggc agaccccaaa ttgtcaataa
     9901 tttccgacac cgtgttccct gttatttcca cctcgctttt tcc
//
LOCUS       DATIAL010000142         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_884796, whole genome shotgun sequence.
ACCESSION   DATIAL010000142 DATIAL010000000
VERSION     DATIAL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884796"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VLA60_04520"
     CDS             <1..196
                     /locus_tag="VLA60_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08654.1"
                     /translation="IELVQQNTLLAGQVEELSAKARQLEENLQLKWLLYGGALVLIGL
                     LIGVILKARPRQTTSFSRYT"
     gene            complement(298..732)
                     /locus_tag="VLA60_04525"
     CDS             complement(298..732)
                     /locus_tag="VLA60_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08655.1"
                     /translation="MQKIATMLLTAALIIGLSGCSYVFYPRADEFAQKAKGTTSVETV
                     LNLTTMMEASAEAAKGGTGHDQPLDDLHNQFHAFDNSLCCVDEAKQETPAYALAVTHN
                     KELWAIFKRLWKFKDTQPQRDEHLALFKTEVQELRATLEALK"
     gene            899..1024
                     /locus_tag="VLA60_04530"
     CDS             899..1024
                     /locus_tag="VLA60_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08656.1"
                     /translation="MKSGKVLAGYAIVDTKRQSSFLNFHRTSTRATFSHYGIDVF"
     gene            1058..1942
                     /locus_tag="VLA60_04535"
     CDS             1058..1942
                     /locus_tag="VLA60_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF08657.1"
                     /translation="MAKLSKRLKTNVEGNFFVDSTCIDCDTCRQLAPDTFSEDGDYST
                     VSRQPETAGDEFAAYQALIACPVGSIGTKKKDRKVFSEAQDSFPLLIEGGVSYVGFNS
                     EKSFGANSYFIQHSQGNWLVDSPRFLKRLVQAFEEKGGIRYIFLSHEDDVAEAARYGK
                     HFGATRIIHRGDASAMPGAEQVVDGDEAVQINRDFFCIPVPGHTAGSLALLYQHRFLF
                     SGDHLWWDRKLQQLGTPERLVWDEAKLEQSVRTLLDYSFEWVLPGHGERIHLPCRDMK
                     KALDQLLERRWNSQHSPA"
     gene            complement(2984..4177)
                     /locus_tag="VLA60_04540"
     CDS             complement(2984..4177)
                     /locus_tag="VLA60_04540"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSF08658.1"
                     /translation="MISLAWWMGMITLVTCSITMTQALAEEPERLPRKKEELQNVSAQ
                     PPKPQPPQWHYGGFVDLGYSHDFNFPDNHLFRNRGTTPRVNELELNMGGAYIRKSASE
                     QSRWGAELLGQGGQDSKAFGFGTNLPHVPGSDVWRHFGRANLSYLAPVGNGLTIQAGL
                     FNSLIGYESLYAKDNFNYTRSWVADYSPYLMFGANAVYPFNDRWTGAVFVINEYFHLQ
                     NANDLPSYGAQAIYTPGRSWTIKETIYYGPDQSNTSLEFWRFFSDTIVEWKGKQVTIA
                     GQYQMGTQKNASVPGNPRLIYMGAALHTRWQITKPWFVALRPELYSDPNGLITGFDQF
                     IWAVTATSEYRLPYGWTNSIFRIEYRHDTSTGSGGGFFKGGQNTLTPSQNLLIFSVIW
                     TFDSP"
     gene            4821..5795
                     /locus_tag="VLA60_04545"
     CDS             4821..5795
                     /locus_tag="VLA60_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HSF08659.1"
                     /translation="MATEKFRLVTRSDFDGLVCAVLLKKVGIIDEIKFVHPKDMQDGK
                     VEISANDITTNLPYVEGVHLAFDHHLSETIRNKGERSNHIIDPKAPSASRVVYRYYGG
                     EKTFPAAWNEMMAAVDKGDSAKYDINEILNPRGWALMNFLMDARTGLGRFKEFRISNY
                     QLMMELIEKCISLSIDEIIALPDVQERVQLFHKHAELAKEQIQRCSTVHDNLVVLDLR
                     DEETIYAVNRFMIYALYPDCNLSIHVMWGLKQQNTVFAIGASIVNRSSMVNIGELCLA
                     YGGGGHRNAGTCQVANEVAGKTLKEIIGMIRLRSMTQVDAGMPAYATA"
BASE COUNT         1443 a         1391 c         1482 g         1502 t
ORIGIN      
        1 catcgaactt gttcagcaga atacactctt agctggccag gttgaagagc tatccgccaa
       61 agccaggcag cttgaagaaa acttacaact caaatggcta ctatatgggg gcgctctggt
      121 actcatcggc ctgttaatcg gagtcattct caaagcacgg ccccgacaga cgacgagttt
      181 ttcccgctat acgtaaggga caagcgaaaa aaattctgcc caaaagtctg tgttccctca
      241 ggcacctctc tcgcacaaaa tcgattcagc cctgtcgggt caatccacgc cgtccgatta
      301 tttcaatgcc tcaagggtcg cacggagttc ctggacctcc gtcttaaata aggcgagatg
      361 ttcatcccgt tggggttgcg tatccttgaa cttccaaagc cgtttaaaga tagcccacaa
      421 ttccttatta tgggtcaccg cgagagcgta ggccggtgtt tcctgtttgg cctcgtccac
      481 acaacacaat gaattgtcga aggcatgaaa ttgattgtgc aggtcgtcca atggctgatc
      541 gtgccctgtt ccgcctttcg cggcttccgc actggcttcc atcatagtgg tcaggttcag
      601 tacggtttcg acgctggtgg tgcctttcgc tttttgggca aattcatccg cacgcggata
      661 aaacacataa ctgcatccgc tgagtccgat gattaaggcc gctgtcagta gcatggttgc
      721 gattttttgc ataaatccct ccatcgtgta tgtgaatgga tacggtaccg tttggttttc
      781 catgtcctgt aaaggtctgc ttgggtaatt cttgttgatc tgattgtgag cctatcttgg
      841 attgcagggt tattcaaccc tctttgaaat ggaaatcgaa aattgaatcc ggatgcccat
      901 gaaatccggg aaagtattag cgggatatgc catcgtagat accaagcggc agtcctcatt
      961 cctcaacttt caccggacat ctacccgagc cacattttct cactatggca tagacgtgtt
     1021 ttagcccgat atactgaaat ccgaagcgaa gcgaatcatg gctaaactct ccaaacgatt
     1081 aaagacgaat gtcgagggca acttttttgt cgactccacc tgtatcgatt gtgatacctg
     1141 tcgacaattg gcccccgaca catttagtga agacggcgat tattcaactg tctcccgtca
     1201 gccagaaacg gcaggggatg aatttgctgc gtaccaagcc ctgatcgcct gtccagtggg
     1261 gtccattggc acgaaaaaga aggatcgcaa ggtattctca gaggctcaag actcctttcc
     1321 gctacttata gaaggcgggg tatcctacgt tggattcaac tcggaaaaat cgtttggtgc
     1381 caatagctat ttcatccaac attctcaagg aaactggtta gtcgattctc cacgtttcct
     1441 caaacgcctg gttcaggcat ttgaagagaa gggtgggatt cgatatatct tcctcagcca
     1501 cgaggatgat gtggcggagg cggctcgcta tggcaaacac tttggcgcca cccggatcat
     1561 tcatcgtgga gacgcttcgg cgatgcccgg tgcggagcag gtggttgatg gtgatgaagc
     1621 cgtacagatt aatcgggatt ttttctgcat acccgttccc ggccataccg caggtagcct
     1681 ggccttgctt taccaacatc ggtttttatt ctccggtgat catctgtggt gggatcggaa
     1741 acttcagcag cttgggacgc cggaacgcct ggtgtgggac gaggcgaaac tggaacaatc
     1801 ggtccggaca ttactcgact attcctttga gtgggtgtta cccggccatg gcgaacgtat
     1861 ccaccttcct tgtcgcgaca tgaaaaaggc ccttgaccaa cttcttgaac gacggtggaa
     1921 ttcccagcac tccccggctt gatcacacaa ttctccccaa caaatcagct tcatccacac
     1981 cactcagggg agaagctcca atttttcaat gagaaaagcg tgaccgaata cgtttctgca
     2041 atttgaatga cgaatttatc tcactaagaa taaatgtccg tcttctatgt tagggcgtaa
     2101 tggttgaata gaaagccaaa tattaggagg ccatgatcat ggccacaaca aattttcctg
     2161 tggcttatgg attatccatc gtcaaatgaa ggagcttggg ttgtatatct ctcacccgcg
     2221 atatcccgag gaaacggata caccttttcc agaacctgga atgtttgcaa tcagtccgga
     2281 cgccagggtc cagttaattg atatttccaa catgcccttc aacaggtccg attccgagga
     2341 attgatcgaa gccttggaag agattcagga aactaataaa ataaacccat caggggaaca
     2401 tatggttaac tttgaagtct cggtatttcc attttttatt cttcttctat tccttcattt
     2461 tggaagtaat cgtttgacgc ttttgtcctc gctttaggtc gagttggggg tttgttctcc
     2521 gcccaaatcc aacccatggc gattaacgtg atcacaatgg tgagactgat ggttattacc
     2581 aaggaccatg tcatgtcaat gcctcacttg attggatgtg taaatattat cttttgcctt
     2641 ggtgaagtaa gtgagcaatc atgatgccac ttggcaaaag aacccttttc cgacaatccg
     2701 gagtttaaac gcttgaattc ttagaagaaa tgaaagttag gcaaccgatg gagggagatc
     2761 gacaaagtgg ttacatcgtg gacaggtgtc tatgaggtca caataaccag ttttctgttt
     2821 ttccttctgt cctatcactt gatgggaaaa accgggcgat agtttctatg tgggtttcct
     2881 gccttgattc ctgtgccgcc atttcagtca gggcgtttct tgtgaaccag tactttccgg
     2941 ggcaatacgc atcctcatct tcatccaggt tcgggatgct ctgtcagggt gagtcgaagg
     3001 tccaaatgac agagaaaatc aataagttct gggaaggcgt cagagtgttt tgcccaccct
     3061 tgaagaaccc gccaccggaa cctgtggagg tatcatggcg gtattcgatc cggaagatgg
     3121 aattggtcca tccataggga agccggtatt ccgaagtggc ggtgaccgcc cagatgaatt
     3181 ggtcaaagcc cgttataaga ccattcgggt ccgaatagag ctccggtcga agcgcgacaa
     3241 accatggttt ggttatttgc cagcgcgtat ggagcgctgc tcccatatag attaatcggg
     3301 gattgccagg aacggaggca ttcttttggg ttcccatctg atactggcct gcaatggtga
     3361 cctgtttgcc tttccattca acgatggtgt ctgaaaagaa tcgccagaat tccagtgagg
     3421 tgttcgattg atcaggccca taatagatgg tttcttttat cgtccaggag cgaccgggtg
     3481 tgtatatcgc ctgggcacca taactcggca gatcgttggc gttttgtaga tggaaatatt
     3541 cattgatgac gaagaccgca ccggtccacc ggtcgttgaa cggatagacc gcattcgccc
     3601 cgaacatgag gtacggtgaa taatcggcta cccaggagcg cgtatagtta aaattatcct
     3661 tggcgtacag ggattcataa ccgatgaggc tgttaaagag tccggcctga atcgtcaagc
     3721 cgttgccgac cggagccagg taggagagat tcgcgcgacc gaagtgacgc cagacatcgg
     3781 acccgggaac atgaggaagg ttggtcccga acccgaaagc tttggaatcc tgtccgccct
     3841 gccccaatag ctctgcaccc catcgggact gctcggacgc actcttccta atataggcac
     3901 cgcccatgtt gagttcgagc tcgttgactc tgggcgtggt gccgcgattg cgaaagaggt
     3961 gattgtcggg aaaattaaag tcatgtgaat atccaagatc cacaaacccg ccatagtgcc
     4021 attgaggcgg ctggggtttc ggtggctggg ccgaaacatt ctgcagctct tcctttttcc
     4081 gtggcagacg ctcaggttct tctgcaaggg cctgagtcat cgtaatcgag catgtcacca
     4141 atgtgatcat tcccatccac caggcaagcg atatcatggt caaatcgttc tccgttttcc
     4201 actgcttccg atcggtcaag ctcgttttga tgctctcccc atctcatgga tgaccggatt
     4261 gaggggacct cgcacctgct ggtttgctgg gtcatgtgaa gatatgtgtc tgacgatagc
     4321 ggtgcccatt acctatagca gcagaaccgt atcaggataa aggctcatgt ggccggtgtc
     4381 atccagaaaa cttttgtacg gtatttttgt ggggaaaaag ggtcatggcc tggaccatgc
     4441 caacaatgga caacgtccag gtaggttctt tcaggccctt cttcaaggaa ataaatgaaa
     4501 tccatgcgtt cgcctgcgtc accgcatttc ttcgcctgga tatagagctc gcgaacctgg
     4561 ttgatctggg gcatcaactg gatttggggc aagggcatcg acttctctaa aagggcgaaa
     4621 aagagttccc ggcttcccgt ttcgggctat gaagtccaac acggaagtca tatatcctta
     4681 agatctgaac ctcatggccg aaaatatgag taggccttgg ccgttcgggc tgcgaacagg
     4741 atgctgcaaa cctcgtcaat cataaacaag cctgtggtat tttcttatca accttgctcc
     4801 ccttttgagg atcaaatctt atggctactg aaaaatttcg attagtgacc cgcagtgatt
     4861 ttgacggact ggtctgtgcg gttctgctga aaaaagtggg aattattgat gagatcaagt
     4921 ttgtgcatcc gaaagacatg caggacggga aggtggaaat ttcggcaaac gatatcacca
     4981 ccaatcttcc ctacgtggag ggtgtccatc tggcgtttga tcatcacctg agtgaaacta
     5041 ttcgaaataa gggtgagcga agtaaccaca tcattgatcc gaaggcccca tcggcttcgc
     5101 gggtggttta tcggtattac ggtggtgaaa agacctttcc ggccgcatgg aatgagatga
     5161 tggccgccgt cgataaaggc gattcggcca aatacgatat caatgaaatc ctcaacccga
     5221 ggggctgggc cttgatgaat ttccttatgg atgcgcgtac cggacttgga cgcttcaagg
     5281 aatttcgaat ctccaattac cagttgatga tggaactcat cgaaaaatgt atcagcctca
     5341 gtatcgatga aatcattgcc ctgccggacg tgcaagagcg ggtgcagctc ttccataaac
     5401 atgccgagtt ggcgaaggaa cagatacaac gatgctcgac cgtccatgat aatctggtag
     5461 tgctggatct gcgggatgag gagacgattt atgccgtcaa ccggtttatg atttatgcac
     5521 tgtatccgga ctgcaatctg tccatccatg tgatgtgggg attgaaacaa caaaacaccg
     5581 tattcgcaat tggcgcgtcg atcgtcaacc ggagttcaat ggtcaatatc ggagaactct
     5641 gcctggcgta tggcggcggg gggcaccgga atgcaggcac ctgtcaggtc gcgaatgaag
     5701 tggccggtaa aacactcaaa gaaatcattg ggatgatacg cctgcgaagc atgacccagg
     5761 tagatgcagg aatgccagcc tatgcgacgg cttaacaggc aatagatgga tggtgtcc
//
LOCUS       DATIAL010000143         8275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1100031, whole genome shotgun sequence.
ACCESSION   DATIAL010000143 DATIAL010000000
VERSION     DATIAL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100031"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(443..1111)
                     /locus_tag="VLA60_04550"
     CDS             complement(443..1111)
                     /locus_tag="VLA60_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08660.1"
                     /translation="MNYSASDHSRTSPLGGILSAILPGLGLLVRGFPVQMTWVLMVGG
                     LLGVATWALGAVGGPGAGWFFGMLMILPWWCLQAYQAYLPTPPGQLATLRLVWKRGHD
                     IRFLGGLFLWTALMDLYIILANPEYHLSVFCTKPTGILGLLFKVQSPVLHIAIGYGFL
                     TLRRWALFVFLAYAGFGILNATTNFACFGFGRIRTVFFISLLAFTAYVIWRRDSLVTH
                     SRPS"
     gene            complement(1580..2203)
                     /locus_tag="VLA60_04555"
     CDS             complement(1580..2203)
                     /locus_tag="VLA60_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HSF08661.1"
                     /translation="MIPRVLLLVIGLFSLLGEELAAQTEGVVLERRYFYKQGIVGVFG
                     IDGEFNPGRYKWSILTPSQESLPGLPAYVDPNRILPFPETSQLPETSTFSALVYEGCS
                     DSNQCEFSFQQTNAPAAKRLVIRLAGIQTPHIKASCEQETLLGRQAQQLIHGYLSSAV
                     HIELISSSIDRKEIVGRLIADDQDLSDLLVSQGLATPVREGKRDWCS"
     gene            2438..4762
                     /locus_tag="VLA60_04560"
                     /pseudo
     CDS             2438..4762
                     /locus_tag="VLA60_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(5466..7268)
                     /locus_tag="VLA60_04565"
     CDS             complement(5466..7268)
                     /locus_tag="VLA60_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSF08662.1"
                     /translation="MELQERLGAWLTSNGCFFRVWSPHADAVAVLVQDGPYWEVDDTV
                     IRQDLVKEGDFWSGTVTGVRAWQLYRFEIKQHGGDSSQRLDPAARDILNSELTRSDPS
                     SRNGSIVLGTEPFPWAPFQTPRFENFIIYQLHIGSFAGRSDQFNKPSSNFLDVESKFS
                     YIREMGFNCIQPLPVHEFAMDRSWGYNPASFFAPESSYGSPYNLAHFVDTAHRQGLAV
                     IFDVVYNHAGPGDNVLWEFDGYTNEGGIYFEGGQQTDWGRGPAWWKREVQDFFYQNAR
                     MFFEDYRADGLRFDVTTQINGEHLKLVLDRIRREFPDKYLIAEHLPPHPWIIKTGRFC
                     ATWHADSHHECQRALAGQDPLNKVRRFLGWDGYDHAWNLVKYTLGSHDDVGDQNNGDA
                     EDGLTNWDKRHRYFVDQLGGRENWEARAKCRLAWALNVAMPGTPMMFMGSECHMASPH
                     VAWGHWHDGWDTHGDHRFNWGIAGDHLGMEMRRLIAACNAVRWQNPALRADSLTIPHE
                     DPTNQVLGFVRQLDDNVVLTVLNLSEQNFADYGYGVRTGGHTGQWTQILCTQDRAFGG
                     WDGAGNAFHEPWTQPDGQAYINLPKWSVVMLRRK"
BASE COUNT         1928 a         2170 c         2289 g         1888 t
ORIGIN      
        1 gtctcctaag ccttccaggg tagataaagg tggttttagg taggacagaa ttttccatac
       61 cacctccaac ctcagcctac tttttatagc atgagttaga tgacctgata tgttctactc
      121 ctatttcttt tatgaaaaac aggtccaaaa atggctttat ggggaacggg ttagtggcgg
      181 gaacaggcaa ggcggtgaac gaaatggtgg accgtgtagg ggggcgaacc taaaggccgg
      241 ctggtcaaaa gttcgaaggg gagtgtaaaa gccagcctgc cattgccttt caggcccaga
      301 tttttgtcat ggctttctgg tctatccccg acttcgtgta aacatctgaa tagcgcaata
      361 ggaggcgagg aggcgtgaca tggagaaacg ctgaaagtct cgccaggagc ttgggcaaac
      421 ttttttcacc caactcccat ttctaggatg gtcgggaatg tgtcaccaaa ctgtctcgcc
      481 gccagatgac gtaagccgta aaggccagga gtgagatgaa gaagacggtg cgaatgcgtc
      541 cgaatccaaa gcatgcgaag ttggtggtgg cattgagaat gccaaagccg gcataggcca
      601 ggaagacaaa tagggcccaa cgacggagtg tgagaaagcc atatccgatg gcgatatgca
      661 gcactggtga ttgcactttg aacaagagac ctaagatgcc ggtcggtttg gtgcaaaata
      721 cggacaggtg gtattcaggg tttgccaaaa taatgtacag gtccatgagg gctgtccata
      781 gaaataaccc tccgagaaat cggatgtcgt ggccacgttt ccagaccagg cgtaacgtgg
      841 ccagttggcc tggtggggtt gggagatacg cttgataggc ctgaaggcac caccacggca
      901 aaatcatcaa catgccgaaa aaccagcccg ctcccggtcc cccgacggct ccgagagccc
      961 aggtggcgac cccgagcagt cctcccacca tcagcaccca ggtcatctga acgggaaacc
     1021 cccggaccag gagcccaagt cctggaagaa ttgccgagag gatcccccca agcggagacg
     1081 tgcgcgaatg atcggatgca gagtaattca cttcagattc catgggaagg aaaatgatag
     1141 agatgtaaga tcctgagggc aagccggact gaggggattg agagggtgcc atcgaaaaaa
     1201 atccggcctc taggaagtga gatttcaacg ccttattcgc cggaacttct actcaattag
     1261 caggcttgtg gaggaaggca agggcgccgt gctgatctgt cctggtctct gatcaagttc
     1321 ggggaggccg gtgttgacca tgaccggcaa ttgaggatca atttaggcta ggttgctgag
     1381 acgttgctat ggtcaaaaaa ctcatccagc ctaggaaaac caaaaacaca tgtatttcca
     1441 tgcggatgaa tgaatagaag ggataggcga aatttgactt cagtggacca tgccgcttgt
     1501 gaccaaggcg gcgcactggc gcgttaccga aaacgtggaa cgtctctttt ttcctcttca
     1561 ccagaaccga tgatgatttc tatgagcacc aatccctttt cccttctcta acgggagtgg
     1621 caagtccttg gctgactaag aggtcggaaa gatcctggtc atcagcaatc aaccgtccca
     1681 cgatttcttt ccggtctatc gaggaggaaa tgagctcgat gtgaacggcg gaggaaagat
     1741 atccatggat gagttgctga gcctgtcggc ccaacaacgt ttcctgttca caggacgcct
     1801 tgatgtgcgg agtttggatg cccgcaagtc ggatgactag gcgttttgca gcaggcgcgt
     1861 tcgtctgttg gaaagaaaat tcgcactggt tgctatcgga gcagccctcg tatacaagtg
     1921 cagaaaaggt ggatgtctcc ggcagttggg aggtttccgg gaatgggaga atgcgattgg
     1981 gatccacata agcgggtagt cccgggagcg attcctgcga aggcgtaaga atcgaccatt
     2041 tatagcgacc cgggttaaac tctccgtcga taccaaacac accgacaatt ccttgtttat
     2101 aaaagtatcg ccgctcaagg actacgccct cggtctgggc tgccaattct tcgcccaaca
     2161 gcgaaaacag accaattacg agtaggagga cgcgtggaat catggtaaga aatcctatca
     2221 cttctgatgt cctggaaaaa gtaggactag gcgatggtag accgcttttg tttgaaaaaa
     2281 gcgtctactt cttccattct cttgaaggtg gactttgcca ttgacggtat gtcctaatcc
     2341 acccacgtgt ttcttcacca tacttaacga ttccatgctt tgcgattgat cagttgttgt
     2401 agtaatcagg aggagcgttt tcatgaaaaa acttcagatg ctgcttttca tcttatttcc
     2461 tgtactcttg cacacaacgg tctccgccca agaagtgctt ccccgaccgg aaggcccctt
     2521 tgagggaaag attggattaa cgtacaaaga ttcccagcct gtgaaacccc ggctgaaggt
     2581 ccctgagacg tttggcttga ccgatccacc caatattctc attgttctgc ttgatgacgt
     2641 gggttatggg cagatgggca ccttcggtgg cgccattccg actcccacct tagaccgtat
     2701 tgccaataat gggttgcgtt acacccggtt ccacaccacg gcgctctgca gcccgactcg
     2761 ggcggcactc ctgaccgggc gcaaccacca ctccgtgggc acgggggtca ttggcgaggc
     2821 cgggacaggc ttccctgggt ataccggcat tatcccggca tcagccgcga catttgcgga
     2881 ggtgcttcgg gaatacggat acgccaatgc ctggtttggc aaaaaccaca atgttccaga
     2941 ctgggagacc agtcttgtgg ggcccttcga tcgttgggcg ggtggattgt gatttgatta
     3001 cttttacggt ttcgtcggtg gtgataccga ccagtttcat ccggccttgg tcgaaaataa
     3061 gaaacgtatt gaaccccctg ataccaatga agatggctcg ccgtatcact tgacgacaga
     3121 tctggctgac cacgccatcc ggatgatccg ggcgactaaa gccgtggccc cccaacgacc
     3181 ggtctttgtg tacttctcca ccggcgccac ccatgctccg caccaggtgc cacaggaatg
     3241 gatcgccaaa ttcaagggac agtttgacat ggggtgggac caatatcgag agcagacctt
     3301 tgcgcgtcaa cagcgtctcg gggttgttcc taagcacgca caactcacca cgcgccctgc
     3361 ctcgttacca gcctgggatt cattgcctga agatgaacgg agggtttatt ccagaatgat
     3421 ggaggtcttt gccgccttca ccgctcatac ggaccacgag gtaggacggc tcatcgatgc
     3481 gatcgagaat ttggggcaac tggataacac ccttgtgatc tatatggccg gggacaacgg
     3541 gtcgagtgcc gaaggaggct tacatgggct gctcaatgaa atgacgttct ttaacgggct
     3601 tcccgaatcg ttggaggcta aggtcgcttc cctcgacacc ctagggggcg agaaacatta
     3661 caatcatttt cccgcggctt gggcctgggc catggacacg ccgttccagt ggacgaagca
     3721 gattgccagc cattttgggg gaacgcgcaa tgggcttgcc atttcatggc cgcagcgcat
     3781 caaggcccga ggggaaatcc gaaatcagtt ccaccatgtc atcgatatcg ccccgactat
     3841 cctggaagcc gtcggcgtgc aagccccggc tcaattcaat ggagttgccc aaaagcccat
     3901 tgaggggatc agcatggcct acacctttga taatccggat attcccggtc ggcgaactac
     3961 ccagtatttc gagatgctcg ggaaccaagg catctaccat gacggatgga tggccagtgc
     4021 gatacgtggg ataccctggc tcagcgagaa cacccctggc gacctgctga acatgtcctg
     4081 ggagctctac catgtggagg aggacttcag ccaagcggtg aatcttgcag ataagaatcc
     4141 ggagaagctc aacagcctcg tcaaactctt ctttgccgaa gctgcgaaat ataacgtttt
     4201 gcctctcgat gatcggaaaa ccgagcggtt gaatgtggca aaccgcccaa gcctcacaga
     4261 aggtcgtaca tcttttacct atccgaactt gcttcggctt cctgaggggg cctcccctga
     4321 tctcaagcac aaggaccata cgattaccgc caaggtggtg atacctgaga gtaaagctga
     4381 gggcatgctc ctcactcagg gaggtcgctt cggggggtat gggctgtatg tgcagaagga
     4441 acggctcatc tatcactaca acctcgttgg tgtcgcacgg tataccatca tgtctaagga
     4501 ccccattccg gtaggagacg tgacgctcaa agccgtgtat aaatcggaca cggacaaacc
     4561 gtttgccgga gccaccgtga ccctttatgc gaacgggaaa cagatcgggg aaggccgagt
     4621 ggagaaaagt attccaaacc gcgtgactct tgacgaaacg ctcgacattg gttttgacac
     4681 gggtacgcag ttgacggaag gatatgagat acccttcaaa ttcacgggcc aattggcggc
     4741 agtgaccatt gaattgaact gagtcatcag tctacttcgg tcccagattg gttgactcta
     4801 gcttttaata ccacaagcgt aagtggtcaa aattttgtct gatccccata tggtcaaaca
     4861 actccaagcc tgccgttaaa ggaacgcgtt cgccggattt ccagaaacag atctaaaaca
     4921 ggttttatgg aaaagagggt ctgggcagga acgagggtag tgcttgattt tattggtggg
     4981 ccgtgtaggg gtcgaaccta cggcccgctg agttaagggg gtaggtgttt tccgtccata
     5041 agactcccta gactacggag cgcctgattc actgattcgg cattggaaaa gcgttcggcc
     5101 acattagggt ctagaacgac cacattcgat ccttgatgat aggccttagc atattttcct
     5161 ctcatcccct gtgaaaaatc atacccccct ccttcattct catcgttttg gattctttgc
     5221 tcctagcttt ctcattgctg gttcgtatca tgctgagcca catctacgtt ttcgataggt
     5281 tccttaaaat gtatttgcct ttaaatgaat ttttggaaac caggctctag cttcggtgga
     5341 cgtttgccaa gagactacca ggctttgccg ggtccgctta acccagtggt agccagaagc
     5401 gactttgcaa aagtgaatcg agatatttcc tgcggcatgt acacgacaat gtggagcgga
     5461 cctgttcatt tccggcgcaa cattacgaca ctccacttcg ggaggttgat gtacgcctgc
     5521 ccatctggct gagtccaagg ctcgtgaaac gcattgccgg cgccgtccca tccgccgaat
     5581 gccctgtcct gggtacatag tatctgcgtc cactgaccgg tatggccgcc cgtccggact
     5641 ccatagccgt aatccgcgaa gttctgttcg ctgagattca ggacagtgag caccacattg
     5701 tcgtcaagct gccggacgaa gccgagtacc tgattcgtgg gatcctcatg ggggatggtc
     5761 agagagtcgg ctcgtaaggc gggattttgc cagcggaccg cattgcacgc cgcgatgagc
     5821 ctgcgcatct ccataccaag gtgatctccg gcgatgcccc agttgaagcg gtggtctcca
     5881 tgtgtgtccc agccgtcatg ccaatggccc caggcgacgt gcggagaggc catgtgacat
     5941 tcggacccca tgaacatcat cggcgtgccc ggcatggcga cgttaagtgc ccacgctaat
     6001 cggcacttgg cccgcgcctc ccagttttcg cgaccgccga gctgatcaac aaagtagcgg
     6061 tgtcgcttgt cccaattcgt aagcccgtcc tccgcatcgc cgttgttctg atcccccacg
     6121 tcgtcgtgag accccagcgt gtacttgacc agattccagg cgtggtcgta gccgtcccag
     6181 ccgaggaatc ttctcacttt gttcagcggg tcctggcccg ccaacgcccg ctggcattcg
     6241 tggtgtgagt cggcatgcca cgtggcgcag aaccgaccgg ttttgatgat ccacgggtgg
     6301 ggcggcaggt gctcagcgat caggtattta tcagggaact cgcgccgtat tcggtcgagg
     6361 acgagcttca gatgctcgcc gttgatctgc gtcgttacat cgaagcgtag cccgtcagcg
     6421 cgatagtcct cgaaaaacat tcgggcgttc tggtagaaaa agtcctgaac ctcccgcttc
     6481 caccaagcgg gaccgcggcc ccaatcggtc tgctggccac cttcgaagta gatgccgcct
     6541 tcgttcgtat agccgtcaaa ctcccagaga acgttgtctc cggggccggc gtggttgtac
     6601 accacgtcga agatgacggc tagaccctga cggtgagcgg tgtcgacgaa atgtgctaag
     6661 ttgtatggtg agccgtagga ggactcgggg gcgaagaagg aggccggatt atacccccag
     6721 gagcggtcca ttgcgaactc atgaacgggc aagggttgga tgcaattaaa ccccatctcc
     6781 cggatgtatg agaacttact ttcgacgtca agaaaatttg acgagggttt gttgaactga
     6841 tcgctgcggc cggcgaagct cccgatgtgt aactgataaa tgatgaagtt ctcaaagcga
     6901 ggcgtctgga acggcgccca agggaatggc tcggttccta gcacgatgga gccattgcgg
     6961 ctgctcgggt cggatcgcgt cagctcagag ttgaggatgt ctcgtgcggc cgggtcgagg
     7021 cgctggctgg agtccccgcc gtgctgcttg atctcgaacc gatagagttg ccacgcccgg
     7081 acacccgtaa cggtcccgct ccagaagtcc ccttccttga ccaggtcctg ccggatcacc
     7141 gtatcgtcga cttcccagta cggcccgtct tgaaccagga cggccacggc atctgcatgc
     7201 ggggaccaga cccgaaaaaa gcaaccgttc gaagtgagcc aggctccaag gcgttcctga
     7261 agctccatcc ggtatctcct cagtttctgt cgtatagaaa actggcctgt gggagatccc
     7321 cccagtgccc gcaggctttc aaaactagag agagaagcct caagaatagc acaacaaacg
     7381 tttacgaaaa cacccgcgaa ccacatgcga tgattggact tgaaggagca catcgagaag
     7441 ttccctctct ctccttactt gtgacttctg gcactttctc ccacgttgga atttcccaat
     7501 ggttttgggt gacacaggat ctggagggaa cgacgtaagt tgctgctttc ctccgtttgt
     7561 ctgggatgag cggaatgaac tttgtgcccc ccgaaaagtt ttcggtcatc cagatcgtac
     7621 aaacagccag ccgtgccggt gagtgtgctg ccaatctggg aatatcggcc aaatttgttg
     7681 gattccagtg ccatggtttc acacagaaga atctgtgttc gcaaatcctg tagcagttgg
     7741 gcagcgtttt ccagcctata ccctgccctt gccaagaagg ccgatttatc gcctcgcgct
     7801 tatggcaaaa gcaattagtg cgtcagcttg tcttcggcga tggttgtgcc cgcggaaagt
     7861 ttcgcagcgt cttcagcggc gcagtataaa aacgttctgc tgcacagtct gcatcctctc
     7921 agtcacgtct gcattgtgaa ccactgcttc aggtgcgggg ggcaaggcgt cagcctaggg
     7981 taggaattct gtggaggcaa gaagactttt agaatttata gtcattccaa taaatttccg
     8041 ccttgttagg agcgaacaaa tgcaagtgcc gggtttcggc cggtagccga ggcccttttg
     8101 ttttggcaaa aggacccaaa accattgacg ccccgcccgg cttcatcgga tgggacggac
     8161 gcaggatacg gagggcggac cacctcgcta cgcttgtatg gtcttttctt gtcattcttg
     8221 agaagctccg aggggtcggg ggtggaggga catcgcttcg catctgccag ttact
//
LOCUS       DATIAL010000144         2434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_455192, whole genome shotgun sequence.
ACCESSION   DATIAL010000144 DATIAL010000000
VERSION     DATIAL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455192"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1022
                     /gene="atpG"
                     /locus_tag="VLA60_04570"
     CDS             150..1022
                     /gene="atpG"
                     /locus_tag="VLA60_04570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSF08663.1"
                     /translation="MASLQALRRKIGSVKNTQKITKAMKLVASSKLRRSQQRILAFRA
                     YGQELRRVLANLSGRVNQSVHPAFQKRPVKIIQVTVVSSDRGLCGAFNMNVFRKAFEV
                     IHECEGRGAKVIIGVVGRKANEFFRRRPWPIRDPNVNVFQRLTYEHALEIGLGGVEDF
                     EQGVFDELYIVYNEFKSIIQQRVVVEKLLPIEPLEEFGAAETASGGGYLYEPDQEELL
                     KTLLPKYAQGQVFRILLESSAAEQAARMAAMDGATRNAGELIKQLSLKYNRSRQEAIT
                     KELMDIVGGAEALK"
     gene            1580..>2434
                     /locus_tag="VLA60_04575"
     CDS             1580..>2434
                     /locus_tag="VLA60_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF08664.1"
                     /translation="MRQDEITKSALEAVTKIGLIALLIIWCFQIIHPFVVPILWGIII
                     AVGIFPIYGWVHHRVTERNWLAATLMTVALLLILVFPVVFLVSVLIDNLRVVSDQLRQ
                     GLLVIPPPPQGIKDWPVIGAPIEKVWGMASENLEVAIQHITPQLRAFISPLLGAAATV
                     GLNILQLIVAVILSGVFLAYSEEGYRLFRAIGRRLAGQQGVELVDLAEATMRSVARGV
                     LGVALIQSTAAGIGLVMAGVPLAGLWTVIALILCVVQIGPFLIMAPAVVYVFSTSDTL
                     PAVLFLIWS"
BASE COUNT          587 a          591 c          635 g          621 t
ORIGIN      
        1 tggttgcggg gatttcccat ttcaaaaatg ggagattttc aaaatttgtg ttcgtattat
       61 tcggacactc ctcagaattc ctaaagagta aaggaagtag ttaatgggtc tgttccaaag
      121 atcatattca agatatggga ggatagggga tggcaagtct tcaggctttg cgccgtaaga
      181 tcggctcggt caaaaataca caaaagatca caaaggccat gaagttagtg gcttcatcaa
      241 agctccgtcg ctcccaacaa cggattctgg ctttccgcgc ctatggccag gaattacgcc
      301 gtgtcctcgc aaacctgagc ggtcgtgtca atcagtctgt ccaccctgca ttccaaaaac
      361 gccccgtcaa gattattcag gtgaccgtgg tctcgagcga ccgtggcctg tgcggggcat
      421 tcaatatgaa cgtgtttcgc aaagccttcg aggtaatcca cgaatgtgag ggaaggggcg
      481 ccaaggtcat catcggagtc gtcggcagga aagcgaacga attttttcgc cgtcgtcctt
      541 ggcccattcg cgacccgaat gtcaacgtgt ttcagcggtt aacctatgag cacgctctcg
      601 aaatcggatt aggcggcgtg gaggactttg agcaaggcgt ctttgatgag ttgtatattg
      661 tctataatga atttaaatct atcatccaac agcgggtggt tgtggaaaaa ctcctcccca
      721 tcgaaccgct tgaagaattc ggagccgctg agacagcatc cggtggtggg tacctgtatg
      781 aacccgatca ggaagagttg ctcaagaccc tgcttcccaa gtatgcacaa ggtcaggttt
      841 tccggatttt attggaatca tcggccgccg aacaagccgc caggatggct gccatggatg
      901 gtgccactcg aaacgccggt gaactcatca agcaactcag cctcaaatac aaccggagcc
      961 gtcaggaggc cattaccaaa gaactgatgg atattgtggg cggagccgaa gcgctgaaat
     1021 aaccccacta ataaaaaaga aattcagaac aagccgggga accccagaga aaaaagatta
     1081 ccctttctgt tatccctcta gcattgccac agcccttggc gtcagatctt acattctaca
     1141 aatcctttga tctccactta cgccttatcc aatcgaatgc aagcatggcc agacctctgc
     1201 gaagcgcctt ttctggtgag ctgtatcatt tctcctcacg gggcagcccg ggagaatatg
     1261 taccggggga taatgaattt ccataatctg gcgagccatt cttgtagatg ccgattggag
     1321 tgagttatgc aaccgtcaat ctggggcaat gccttgtgaa gtgagtaaaa tgtaaaccct
     1381 gctcccctac cctgccccca aacaattgga ttgttcaagg ggaataatct aagatacttg
     1441 aaatttttct gctaaaagta attttctcga ggcaatctac agcagttcat aagaaattta
     1501 cgaagaatta cccttctctt aatccgacac gattatccct cttaaaaagc taaacccgat
     1561 cgaaatgaag tgaggctcca tgcgccagga tgaaatcact aaaagtgcat tggaagctgt
     1621 cacgaagatc ggcctgatag ccctcctcat catctggtgt tttcagatca tccacccgtt
     1681 tgtagttcct atattgtggg gaatcattat tgccgtggga atctttccga tatatggctg
     1741 ggtgcaccac cgggtaacag agcggaactg gctggcggcc acattgatga ccgtggcttt
     1801 actgctgatc ctggtttttc ccgtggtgtt tctggtctcg gtgctgatcg ataatctccg
     1861 cgttgtctct gatcaattac gtcaaggcct gctggtcata ccgccgccgc ctcaagggat
     1921 caaagattgg ccggtgatcg gcgctccgat tgaaaaggtc tggggaatgg cgtccgaaaa
     1981 ccttgaggta gccattcaac acatcacccc gcagcttcgt gcatttatct ctccgctcct
     2041 gggagcggcg gccacggtgg ggttaaatat cctgcaatta atcgtggcgg tgattttatc
     2101 aggagtgttt ctggcttatt cggaagaggg atatcgacta ttcagggcca ttggcaggag
     2161 attggccggt cagcaggggg tggagcttgt agaccttgcc gaagccacga tgcgcagtgt
     2221 ggcgcgaggt gtccttggcg tggctctgat tcagtcgacg gcggctggaa ttgggctggt
     2281 catggccggg gtaccgcttg cgggcttatg gacggtgatc gcgctcatcc tgtgcgtggt
     2341 tcagatcggt ccctttctca tcatggctcc ggctgtagtc tatgtgtttt cgaccagtga
     2401 cacacttccc gcagtgttgt ttttgatctg gagt
//
LOCUS       DATIAL010000145         3396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_992646, whole genome shotgun sequence.
ACCESSION   DATIAL010000145 DATIAL010000000
VERSION     DATIAL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992646"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VLA60_04580"
     CDS             <1..640
                     /locus_tag="VLA60_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HSF08665.1"
                     /translation="FERVQAAIQVLQELGGNIQEVTLPSTDRAIATYYLLATAEASSN
                     LARYDGVRYGFRAKESRELAEMYRNTRSQGFGPEVKRRIMLGTYALSAGYYDAYYGKA
                     QAVRTLIQRDFETVFKDVDLLVSPVMPTPAFQLGERLDDPLQMYLSDVYTIPASLSGL
                     PAISIPCGFSTMGLPIGLQLMGRPFEESTVLRAAYAYEQATNWRGKRPVIRS"
     gene            655..1062
                     /locus_tag="VLA60_04585"
     CDS             655..1062
                     /locus_tag="VLA60_04585"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HSF08666.1"
                     /translation="MVDLAAIIIAVAFVVLVGYLVPMILQVKRTIGQSERMLIRLNHE
                     LPGLLKDVKGTNENIRALTDQARLGVDRATIFLNAVGDVGQTVNHVHQAVRGKGGALV
                     VGFTSLLAGLKAATSTVKKRVHKEQEGGPYYGD"
     gene            1052..1366
                     /locus_tag="VLA60_04590"
     CDS             1052..1366
                     /locus_tag="VLA60_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSF08667.1"
                     /translation="MATNHSGASAGSVALAFLSGALLGAVTAIMLAPQPGRESRERIT
                     GYARRTGEDLRDLKERATDAVEDVVGRGRELVDEISCALREALDAGRDAMRREREMKS
                     LD"
     gene            1409..2839
                     /gene="gatB"
                     /locus_tag="VLA60_04595"
     CDS             1409..2839
                     /gene="gatB"
                     /locus_tag="VLA60_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSF08668.1"
                     /translation="MAYEPVIGVEVHAQLLTKTKLFCGCPTSFGLPPNTQACPTCLGL
                     PGSLPVLNQRAVEMAVRTGLALHCEIAGTNQFSRKNYFYPDLPKGYQISQYDLPICGP
                     GWLDIAVNGEKKRVRIRRAHLEEDAGKNVHDVSVEGSGVDVNRAGTPLLEIVTEPDLH
                     SSDEVDAYLKTLRDLLVYLEVCDGNMEQGSFRCEPNVSLRLQGQNSLGTKVELKNINS
                     FRFAKEALDFEISRQTEVLTEGGTIRQETRLWDMQKEHTVVMRSKEEAHDYRYFPDPD
                     LLPIHVEAEWIEVLRHTIPELPAARLQRFVKDYHLPEYDAKILTDSKGLADYFESCVK
                     LFLHPKTVSNWVMGELLRELNATGTSPESSPMTPERLVHLLQLVEDGTISLKVARDLF
                     PELYASGKDPDQLVKEKGLAQVSDEGMLEKIIEQVLAQYPAQVEQYRAGKETVLGFLV
                     GQVMKASQGKANPGKVNTLLKHHLIS"
     gene            2909..>3396
                     /gene="eno"
                     /locus_tag="VLA60_04600"
     CDS             2909..>3396
                     /gene="eno"
                     /locus_tag="VLA60_04600"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSF08669.1"
                     /translation="MSLIQHIHSRMILDSRGNPTVEVDVHLASGMSGRAAVPSGASTG
                     TREALELRDGDKKQWMGKGVSKALAGIQKHMLPALRGMEAQDQVTIDSRLIQLDGTKG
                     KTRLGANATLGVSLAVARAAAQELGVPLFRYVGGLSARELPVPMMNIINGGAHADNGL
                     DL"
BASE COUNT          836 a          741 c          970 g          849 t
ORIGIN      
        1 attcgagcga gttcaggctg ccatacaagt gttacaggag ttgggcggca acattcaaga
       61 agtgacactg ccatctaccg accgggccat cgcgacctat tacctccttg ccacggcaga
      121 ggccagttca aatttagccc gttatgacgg tgtaaggtat ggatttcggg ccaaggaatc
      181 gagagaattg gcagagatgt accggaatac ccggtcgcag ggattcggac ctgaagtaaa
      241 gaggcgtatt atgttgggaa cctatgccct gagtgccggg tattatgatg cgtattatgg
      301 aaaagcccaa gcggttcgaa ccttgattca gcgtgatttt gagactgtgt ttaaggacgt
      361 tgatctcttg gtcagtcctg tcatgccaac cccggctttt caattaggtg agcggttgga
      421 tgatccctta caaatgtatt tatccgatgt gtataccatc ccggcaagtt tatccgggct
      481 ccccgctatt tctatcccct gtggatttag tacaatgggt ctgccaatcg gattgcaatt
      541 gatgggacgt ccgtttgaag aatccacggt cttacgcgct gcatatgcct atgaacaagc
      601 gacaaactgg cgagggaaac gaccagtgat ccggtcatga aaggatagcg aggtatggtg
      661 gatttggcag caatcattat cgcggtggcg tttgtggtct tggtgggtta tttagttccc
      721 atgatccttc aagtgaaacg caccattgga caatccgagc gcatgctcat tcgtctcaat
      781 catgaattgc ctggattatt aaaggacgtc aaagggacca acgaaaatat tcgggctctg
      841 acagatcaag ccaggcttgg cgttgatcgg gcgaccattt ttttgaatgc cgttggagat
      901 gtcgggcaaa ccgtgaatca tgtccatcag gctgttcgtg gaaaaggggg ggccttggtg
      961 gtgggcttca ccagtttgct tgccggactc aaagcggcaa cgtccaccgt taaaaaacgt
     1021 gttcataaag aacaagaagg gggaccatat tatggcgact aatcattcgg gtgcatcagc
     1081 tgggtcggtc gcattggcat ttttaagcgg ggcgttactg ggtgccgtca ccgcgatcat
     1141 gttagcccct caaccgggtc gcgagtcacg tgaacggatt acggggtatg cacggcggac
     1201 gggtgaagat ctgcgggatt tgaaagaacg ggccacagat gccgtggaag atgtggtcgg
     1261 tcgtggacgg gaattagtgg atgaaatctc ttgcgcactt cgggaagcgt tggatgccgg
     1321 tcgggacgct atgcgtcggg agcgggaaat gaaatctctg gattaaattg cttgaagacc
     1381 ggattgagag gagggcattt gccgcgccat ggcctatgaa cccgttatcg gagttgaggt
     1441 gcatgcccaa ctcctgacga agacaaaatt attttgtggg tgtcctactt cattcgggct
     1501 tcctccgaat acccaagcgt gtcctacgtg tctgggattg cctgggagcc ttcccgtgct
     1561 gaatcaacgt gcggtggaaa tggctgtccg cacgggatta gcgcttcatt gcgagattgc
     1621 cggcacgaat caattttccc gtaaaaatta tttttacccc gatcttccga agggctatca
     1681 aatttcgcag tatgatctgc cgatttgtgg accggggtgg ctggatattg ctgtcaatgg
     1741 agaaaaaaaa cgggtccgca ttcgtcgagc acatttggaa gaagatgccg gaaaaaatgt
     1801 gcatgatgtg tcggtggagg ggagtggagt ggatgtcaat cgtgcgggca cccccttgtt
     1861 agaaattgtg actgaaccgg atttgcattc ttctgatgag gtggacgcct acttaaaaac
     1921 tctgcgtgat ctcctggtgt atctggaagt ctgtgacggg aatatggaac agggaagttt
     1981 ccgttgcgaa ccgaatgtat cgcttcgcct gcagggccag aatagtctgg gaacaaaggt
     2041 ggaattaaag aatattaact ccttccgttt tgctaaggag gcgctggatt ttgaaatctc
     2101 caggcaaacc gaggtgttga cagaaggagg aacgattcgt caggagaccc ggttgtggga
     2161 tatgcaaaag gagcatacgg tcgttatgcg tagtaaggag gaagcgcatg attaccggta
     2221 ttttcccgac ccggacttgc ttcctatcca tgtggaggct gaatggatcg aggtattgcg
     2281 tcacaccatt cccgaattac ctgccgctcg cttgcaacgc tttgtaaagg attaccatct
     2341 ccctgagtat gatgcgaaga ttttgaccga ttcgaaaggt ctggccgact attttgaatc
     2401 gtgcgtgaaa ctttttctcc accccaaaac cgttagtaac tgggtcatgg gcgaattact
     2461 tcgtgagctc aatgcgactg gaaccagccc tgaatcttcg ccgatgactc cggaacgatt
     2521 ggtccacctc ttacaattgg tggaagatgg gaccatcagt ttaaaagttg cgagggatct
     2581 ttttcctgaa ttgtatgcca gtgggaaaga tcccgaccaa ttagtcaagg agaaaggtct
     2641 agctcaggtt tctgatgaag gcatgcttga gaagatcatt gaacaagtgc tggcccagta
     2701 tccagctcaa gtggagcaat accgggcagg aaaggaaacg gtattaggtt ttttggttgg
     2761 gcaggtgatg aaggcctcac agggaaaagc gaatccaggt aaagtcaata cgctgttgaa
     2821 acatcacctc atatcttgat ctaagacttg tctcagggta caaaggtata cgagaggatc
     2881 attatttact ccaaggaatt ttttttctat gagtctcatt caacacattc actcacgtat
     2941 gattctggat tctcgcggaa atcccacggt agaagtggac gtacacctgg ccagtggcat
     3001 gtcaggtcgt gcggcggtcc cctccggagc ctcgaccgga acgcgagaag cattggaatt
     3061 acgggatgga gataaaaaac agtggatggg caagggcgtc tctaaggcct tagcgggaat
     3121 tcaaaaacat atgctacctg ctctccgggg catggaagcc caggatcagg tgactatcga
     3181 ttcacggtta atccaactgg atgggaccaa aggcaaaaca cgattagggg cgaatgccac
     3241 tcttggcgtc tcgttagccg tcgcacgggc tgcggcacag gaactcggcg ttcccttgtt
     3301 tcgatacgtg ggtggactgt ctgcgaggga attgccggtt cccatgatga atatcatcaa
     3361 tggtggtgcg catgctgata atgggctgga tctcca
//
LOCUS       DATIAL010000146         3473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_348559, whole genome shotgun sequence.
ACCESSION   DATIAL010000146 DATIAL010000000
VERSION     DATIAL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348559"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..689
                     /locus_tag="VLA60_04605"
     CDS             198..689
                     /locus_tag="VLA60_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08670.1"
                     /translation="MPKQIKTIMGCLLVVFLVVGFYMLNRFVQPTEYRLPDDPHALEA
                     LELVKKHRSRQGYTLAEAIQVLVDDLEAKDIPFREGGWQVSSQGEDHYVVRKIIREKG
                     SVEWIEREYAWRVDTKEKSIRVISLAAQQLMPFENLPPLPHGDQISLLPSIQPPVPVA
                     RHG"
     gene            complement(649..978)
                     /locus_tag="VLA60_04610"
     CDS             complement(649..978)
                     /locus_tag="VLA60_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HSF08671.1"
                     /translation="MKFYPVAKITDLPPGSCKSLELLDEGIALFNLNGTVYALDNTCP
                     HAGGPLGGGSIEGDFVECPWHGWKFNIKTGACKKNPGPTWNVRCFPVQVIDGVIHVAP
                     PEPEVGY"
     gene            1128..1664
                     /locus_tag="VLA60_04615"
     CDS             1128..1664
                     /locus_tag="VLA60_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSF08672.1"
                     /translation="MEMTLLLNATYEPLRVVHWQKAINLLWQGKVEVLEFYDRDIRGV
                     SISFKLPSVMRLLNMVKLRSNHHAVKFSRINIFTRDRYTCQYCYKRFRTEELTFDHVI
                     PIAKGGRKTWENIVTACWRCNNRKSGRIPDEAGMKLLKEPVKPKWTPRLTLMIGIRHA
                     PESWRDYLYWNLELDADP"
     gene            complement(1940..2092)
                     /locus_tag="VLA60_04620"
     CDS             complement(1940..2092)
                     /locus_tag="VLA60_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08673.1"
                     /translation="MHRCEKCQGTMLLDREIDMESGMSLLVLWCINCGLRKQAERAPI
                     PLIEVS"
     gene            2416..>3473
                     /locus_tag="VLA60_04625"
     CDS             2416..>3473
                     /locus_tag="VLA60_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08674.1"
                     /translation="MITVNVGEGGLDMLSDVQSSAPAEPEWVTWSDEQLLDLRLCDLK
                     IRIPGTELGKCVRQLYRELKSQGLSFRPHVWLSDDWYSPDGVPGLAVPFYMGHPRLAK
                     LELNQMLEVEGGTPEWCMRILRHETGHAIENAYRLRRRRSRQQIFGHTSVPYPDCYLP
                     KPYSKSFVLHLDMWYAQSHPDEDFAETFAVWLTPHSHWRERYAGWPALKKLEYMDGLM
                     KELVGLPPLVTTKAQVDPIRRLQKTLREHYQSKRERYGLDYPNFYDRDLRRLFSGDPD
                     QSANPSAANFIRKFRKEVRRKVAEWTGEYQYTIDQVLQDMMAHCRERNLRLAVPEEQA
                     KVDFTILLTVQTMNYLHGG"
BASE COUNT          937 a          759 c          879 g          898 t
ORIGIN      
        1 cgaagcaaac aagatcggga tatgtcagat tggcccataa ttttgaacta tccaaaagtt
       61 gaagaaaatg gcagggccat gtatggaaaa ttatgctata acatcataca aattccgaat
      121 ttactggggg gaattgcgag aatcggaaat tattcgggga gaaaggatca agagtgttca
      181 tgaaatggat gaatctcatg ccgaaacaaa taaagacaat catgggatgt ctactggttg
      241 tatttctcgt ggtagggttt tatatgctga accgctttgt tcagccaacg gaataccgtc
      301 taccggatga cccacacgct ctggaggctc tggaattggt aaaaaagcat cggtcccggc
      361 aagggtacac ccttgctgaa gctattcagg ttctggttga tgatctggaa gccaaggaca
      421 ttccctttcg agagggaggc tggcaggttt catctcaggg cgaagatcat tatgtggtac
      481 gtaaaattat ccgggaaaag ggatctgtgg agtggatcga gagggaatat gcctggagag
      541 tggataccaa ggaaaaatcg attcgagtga tatccttagc agcgcaacaa ttaatgcctt
      601 ttgaaaatct gcctcctctt ccccatgggg atcagatctc tcttcttcct agtatccaac
      661 ctccggttcc ggtggcgcga catggatgac gccatcgatg acctgaaccg gaaaacaccg
      721 tacattccag gtggggcctg gatttttttt gcatgctccc gttttgatgt taaacttcca
      781 tccgtgccag ggacattcga cgaaatcccc ttctatcgag cccccgccca atggtcctcc
      841 ggcatgcgga catgtattat caagagcata gacagtgcca ttgaggttaa agagggcaat
      901 accctcgtca aggagttcaa gactcttgca gctaccggga gggagatccg taatcttggc
      961 aaccggataa aacttcattc ttcatctcgt gcgagggaaa aacccctaat atatcaactt
     1021 cttccttgcg caacaacaga ctcatggcgt ctcttgattc taaggatgag tcggtgatat
     1081 aggaacagag tttatccaac ctggcatata ttaatgggtg aattggtatg gaaatgacct
     1141 tgttactgaa tgccacctat gagcctttgc gagttgtgca ttggcaaaaa gccattaatt
     1201 tgttgtggca aggaaaagtt gaagtccttg aattttatga tcgggatatt cgaggagttt
     1261 ccatttcatt caagctgcct tccgtgatgc gtctcttaaa tatggtcaag cttcgatcaa
     1321 accaccatgc ggtgaaattc tctcggatta atatttttac ccgtgaccgg tatacctgcc
     1381 aatattgcta taaacggttc cgcaccgaag aattgacctt tgatcatgtc attcctatag
     1441 caaagggtgg aaggaaaact tgggaaaata tcgtgaccgc gtgctggcgc tgtaataacc
     1501 ggaaaagcgg ccgtattccg gatgaggcgg ggatgaaact actcaaggaa ccggtcaaac
     1561 ctaaatggac tccacggctc actctaatga ttggcatccg tcatgcgccg gaaagttggc
     1621 gggattattt atactggaat ttggaactgg acgcggatcc ctgatcgctt gatcctctta
     1681 aattttcctg tttcgtgctg cgaactcatg ccttgcctcg ctcagctgaa cgtctaataa
     1741 gtgtcagacg atcttacgtc tcacacgaat ggatactctg tcggttctcg cgttagagca
     1801 ggatatggca atcagatgaa cgtcccttac gcatgtcccc accaaattac tgagaaaata
     1861 tcaggaataa catttatctt atctttgctc agccctcggc gcgcatgtgc tttggaaggt
     1921 tcgaagatta agccgtttgc taggaaactt caataagggg aatcggcgct cgttcggctt
     1981 gtttccgcag gccgcaatta atacaccata agaccagaag agacattcct gattccatgt
     2041 cgatttcccg atcaagtaac atcgttcctt ggcatttttc acagcgatgc atagattaaa
     2101 tctcctgtgg ttcttccggt caagctgaag agtcgtaaca atcaacatgt gaatggcata
     2161 caagcagaat agtccatggt gaatggacac cataaatgaa taaagccgag aaatgattct
     2221 tcagaaggct gatccggagg gtgaatggaa agttcccaaa aaagtggggc aatctaccat
     2281 agctaaattg gtaaaagcta tggagatgaa gaggtgaagt aaataaatct gaggcatgga
     2341 ttatgctttt aaaaaggaat gcagtgaaga tttgaatatg ataatcccac tcgtttcgcc
     2401 ggcggagaag gtacattgat aacggtcaac gttggcgaag gtgggttgga tatgttgtcc
     2461 gatgtccaat catcagcacc cgcagagcct gagtgggtca catggtcaga tgaacaactt
     2521 ctggatcttc gcttatgcga tttgaagatc cgtattccag gaacagaact gggcaaatgt
     2581 gttcgtcaac tgtaccgcga attgaaaagc caaggattgt ccttccgtcc ccatgtctgg
     2641 ttgtctgacg attggtattc tcccgatggg gtgccgggtc ttgctgtccc tttttatatg
     2701 gggcatcccc ggttagccaa gctggaactg aatcaaatgt tggaagtgga ggggggaacg
     2761 ccggaatggt gtatgcggat tcttcgccac gagacgggcc acgccattga aaacgcgtat
     2821 cgcctgcggc gtcggcgtag tcgacaacag atattcggcc atacctcggt tccctatccc
     2881 gactgttatt tgccaaaacc ctatagcaaa agtttcgtgc tgcatctgga catgtggtat
     2941 gcacagagtc accctgatga agattttgca gaaacatttg cggtctggtt gacgcctcac
     3001 tcccattggc gtgaacgata tgccggatgg ccggcactca aaaaattgga atatatggat
     3061 gggcttatga aggagttggt gggattgccc ccactggtga ccaccaaagc tcaggtggat
     3121 ccgattcgcc ggcttcaaaa aactcttcgt gaacattatc aaagcaaacg ggaacggtat
     3181 gggctggatt accccaattt ttatgatcgg gatttgcggc ggttattttc cggggatcct
     3241 gaccaatcgg ctaatccgtc cgcggcgaat tttatcagga agtttcgaaa ggaggttcgc
     3301 cggaaagtcg ccgaatggac cggagaatat cagtacacca ttgatcaggt tctgcaagat
     3361 atgatggctc actgccgcga acggaacctg cggttagcag taccggaaga acaggcgaaa
     3421 gtcgacttta cgattttgtt gacggtgcaa accatgaatt acctgcatgg tgg
//
LOCUS       DATIAL010000147        18068 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_992858, whole genome shotgun sequence.
ACCESSION   DATIAL010000147 DATIAL010000000
VERSION     DATIAL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992858"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VLA60_04630"
     CDS             <1..595
                     /locus_tag="VLA60_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSF08675.1"
                     /translation="PDQPSSNPTLGRRMFLRQSAISIGATVHEFVKHRDAPSPIKKEQ
                     PVPEKTGWLRPPGAVDEELFLERCTGCADCVEVCPHDAIQVLLSNDTPVIYPGEIPCK
                     LCEDLPCIGACTTEALLPVADRFQVNMGVAKVFSKICTAEAGCNACVSKCPTEALSMD
                     FASYHIRVDDSVCVGCGICQYICGTVNDRSAIRIVSR"
     gene            931..1902
                     /locus_tag="VLA60_04635"
     CDS             931..1902
                     /locus_tag="VLA60_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HSF08676.1"
                     /translation="MTSGVKEGKKAGKNGMPVSGNEKAVKISPKDAPAVDRALCRSKL
                     YLLVSWGYLYPEDEEFLDYLQSGEFVEDGKTALEGLKKELQNIGGDEAQERLDALVGN
                     FDAIEAWISSEGENWSLQDLRDEHRRVFSNVIALDCPPYETLFGNDHVFGQSYVMGDI
                     AGFYSAFGLQLSPDIHERLDHLSVELEFMHYLAYKESYAILHDGAEKLKTVIEAEKKF
                     VKEHIGRWVPLFSGMLNRKADYGFYKILADFTSHWIAFDIAFLGVTPQPYSETDYRPA
                     SYNNPEGQSFECGAQDQGNELSLLMSEVGADAFLDKESGTSGQSGNA"
     gene            2019..2975
                     /locus_tag="VLA60_04640"
     CDS             2019..2975
                     /locus_tag="VLA60_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSF08677.1"
                     /translation="MRLVQTRNKRLVFGVLLSVLVVCVGLTMGQIPLAVSQPVTIPVG
                     KITGPIPMDAANPVWESVPGIVAPLSGQTITTPMHPNISVKTVMIKMATNGEEIGVWA
                     NWGDQTLNNTTIGPQDFRDQVAVQFPVQSAGAPPFQCMGQSGGTVNIWRWNAEWQKDL
                     GAGVAGMWDVDNQYPSIAWDYYYEEPAGGVTYPDRIGRSLGPFNPGIWSGNIMSDPNL
                     RLGSVEDLNANGFSTLTTQASQDVVGNGLWEPYGSLKGGCCSGPTWRVVMKRSLKTQD
                     PNDVQFAAGASFPVAFAVWDGSNVERNGMKGISTWFTAQMPN"
     gene            3226..4998
                     /locus_tag="VLA60_04645"
     CDS             3226..4998
                     /locus_tag="VLA60_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF08678.1"
                     /translation="MKISLLFPPSWHPSQPYLSVPCLSAFLEKSGVPVHQQRDLNIEL
                     LDRVLTKGFGLDIYPRLVDLVKKLEVSVSGDTGYGSKEHYARVVESLDRFPYLIDEIE
                     PVKASLRGEEFYDIERYRECLFLIDRWLEVVSSLYFPTRMTVVDNQFAYSIYSSREIL
                     QAIHDENQNPYLELYRKFFLPAFGQDISSFVGVSITATSQIIPGLTLCKLIKENHPEV
                     HVTVGGSIFTRLVDNLRRCDRLFQLTDDFIVFEGETALLELINQLDGKKDFTKVPNLI
                     WRQDGKIIVNQPFYSENLADHPAPNYDGFPLSDYLAPHTVLPVQFSRGCYYKDCAFCA
                     LTLDHQNFRQKDPIKVVDELEALSHKYETPYFFFTDECLALSPTKRLCKELIHRGLGL
                     QWTAELRFEKNLSRELLTSMREAGCQKIVFGLETYNARLMDFMVKGITQENVDRICSD
                     CVDLGIAVHCYVIVGFPTETEEESLETLNFIVNNTKLHSSYGFSCQPCLFDLEKEAPI
                     MSDPGGYGIQRIMRPASEDLSLGFFYEVKSGMTPEQAEKVYQYVYEKISEVVCELPFN
                     YSMADGLLYITRHNKELKVEPSRV"
     gene            5077..6189
                     /locus_tag="VLA60_04650"
     CDS             5077..6189
                     /locus_tag="VLA60_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08679.1"
                     /translation="MISTTAKNQERIDGPGLLFEWGLKSLLFAAFFELVLYRLVSRLG
                     MHLGKLAEKYEAVRIGFRTLSSLGFVLLNMTAILVFLILGILLVQKLRTKLWVGEWDK
                     VVIPLAALLVACTVGYLFFPPVMVGTIVYNILAFGLIVFLVLEYWATHRLLSQRLMVG
                     SFFLGISGWLYYQTMSTTYGWLNIFDAPPLVHEGNRLGEALMVLSSILVFWAYGTTSI
                     ITKNKQQRRWGLTLVISGGSAFVALLFMDFFLSLWNPEVAKEVRKAGEGIGWIFQMGM
                     GYTFYLPFAFYVLGFTCWAYTVIKLVLIGRLAGYGLGLMFMAGYALQLSHLTLMVVLG
                     LMLLNIDKRGTLGKLRKKTQEGVVYQPIRPVYGQTQ"
     gene            6204..7019
                     /locus_tag="VLA60_04655"
     CDS             6204..7019
                     /locus_tag="VLA60_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF08680.1"
                     /translation="MEEQGAGSQTMDPQSGETTTEERPLTVDEEIAEIEKLLSEEPDD
                     FQARCRLGELYFNKGRMDEALAEVKKSIEMAEGIRIEMDRSLAMYYSNLGTIYGTKGM
                     LDEATAQFKRALELNPYDVLALFNVGRLYNDKNAFLEAKEYFERLIEISPEDPIAWYN
                     LAGVYEKLDDPNVSDFNTIDMAMQAYMKVLQFDPSHLESSFKLMEIALNLKKPDMAVK
                     IMEDAVENNQDEPLAYYNLINTYEKCKMFEQAEETRNRLKDRFNKRSRETVKK"
     gene            7042..7179
                     /gene="tatA"
                     /locus_tag="VLA60_04660"
                     /pseudo
     CDS             7042..7179
                     /gene="tatA"
                     /locus_tag="VLA60_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            7454..8707
                     /locus_tag="VLA60_04665"
     CDS             7454..8707
                     /locus_tag="VLA60_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08681.1"
                     /translation="MSTETALEIGVIETFAGNGKSRSTGDGKRAVKSGIPLPNHITVD
                     RHGRFFYIAESGSDRIRRVDMETGLLHNFAGTGETCYSGDYGPCGEAGLYLPLDVLCD
                     SHNHIYVCDSGSNRIRKIDADTGIITTIVGTGQWGYNGDGPALECNLTYPAAIAIDDQ
                     DHLYIADTQAHRIRHYNPQTGMLTTIAGCWTAEDDERVSPLTAQNLIVLSGDAIGIDF
                     SNDEGLLRPRCSDGLDLSLYLDDGKPALECKLYDVVDIDVDKHGDLYLTDKGTNRIRK
                     LDRKTGTMSTVAGICRYGFDGDGKLAVKSMLNVPEGAVVDSDGNVYISDSMNHRVRKV
                     DCQTGIITTIAGNGDSGYDDKNMGGCGAARFVAKEDAGMLKHGDGLIAIEAIVNTPAG
                     LTLDSQGYLYICERNENKIRRVKLR"
     gene            9092..9970
                     /locus_tag="VLA60_04670"
     CDS             9092..9970
                     /locus_tag="VLA60_04670"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSF08682.1"
                     /translation="MSTIHAPKRIVLFVVFALVVLFGLTWYQVPLVSSQGMVVLAHFS
                     KGEVPSHPSDSAWETVAPLPVPLSGQIITRPVWPEPSARALSIRSIHNGKDIAFLLEW
                     QDATINESLTPGVFRDGVAVALPLGDAPAFFCMGQLDHYVNIWHWKADWQSDVDRRAE
                     RAKESKRGESGPRRFEVIPRRPSSVEDLVGGGFSTLTSKEQQGRIQGQAEWKNGLWRV
                     VMKRPLTSMGDDLDNEAMLVPGRMQAIAFAVWNGENKERNGQKSVASWMQLLIDPVQP
                     ASPNGAGGIESTHSSS"
     gene            10051..10440
                     /locus_tag="VLA60_04675"
     CDS             10051..10440
                     /locus_tag="VLA60_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSF08683.1"
                     /translation="MVLQGEAFGQPPSENMTNEKALILAEKFGIDVGEVDEEVKKILG
                     LTKAEGVVVYAVIGGSPAELSGIKVKAIIKEVDKYEIKTLVDLGTALEQTLPTKNFTV
                     ATYEPADPDNQGVTGGLNFHFVRILQD"
     gene            10450..11787
                     /locus_tag="VLA60_04680"
     CDS             10450..11787
                     /locus_tag="VLA60_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSF08684.1"
                     /translation="MKNLFSKNFRLLEIIPMHNRWILSIILGIFCLSDVGFGQQSSTV
                     VGEAEESWIKEIEQVFIPSEQCKQCHDRHYEEWKGMREQTMDLKTFGRVDGALLHGTA
                     FTSPVFKTVLGLWLHTDPDQEQRTRCLSCHAPAVTVFPQHTDRIIDQVMKGEKRVKIE
                     GIGCSACHLITGIQENAYGHPTFKITSGKTLYGPYAEPEENLVHPSGQVDIYRGAHYC
                     ASCHFDKVRDVTRPDIPGEILKGTICQDCHMERSTGSSTSQRGALTRPIGRHWFQGIV
                     IPGIMLSNRNLQAEWFSRVDIEVEKVKGQVEGEVLVRNGALPHTFPGGDPVLKQFYVT
                     ITLKNSDGQVIDQYQERFGRTFEELLRGPIPRPFVNGGTTRHIPFTLKYPQDSEASLI
                     EASVSYSLIPEPSKALAAKFLESLDTDKEREAAERIIHDYSSPRLLTFRTMSL"
     gene            11838..13091
                     /locus_tag="VLA60_04685"
     CDS             11838..13091
                     /locus_tag="VLA60_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSF08685.1"
                     /translation="MRKSYKKSAPTGMRGGVFLLVLGCFLLTAPLPGSVAGESSSTKK
                     PLEKSFPNSEKCKRCHLRVFEEWEASAQSRSIVTAPFRVTLDQFLASTDKKDHAMCFR
                     CHAPHILEYADHLPRFIKEVQSKDPQMDGVGCPQCHLIQDVDMNSHPPTPTFQLGTTI
                     FGGYDKAAENLAHQSQKLNLYRESKFCVTCHDSLPKISGSAKDLPGWLGSWEKTKSET
                     NGKPCQSCHMPEAVDESANGEKVRKVANHSFPGRFGKVRAEAVTLDFTTETTPETSQV
                     QVSIQSLVPHNLPLPHPGWSRVVVDLSIFGKNLKKVYNEQRFYERTFGNGEEKETVFD
                     FEAKKVLRDTLLKPEEMRVEVFTFPTPQDAPSMDVVVTLTYAPVHGPEDFLKKVEQEA
                     ALGQKDKAFQSVQIAQKKSNVPLKK"
     gene            complement(13117..14790)
                     /gene="ilvD"
                     /locus_tag="VLA60_04690"
     CDS             complement(13117..14790)
                     /gene="ilvD"
                     /locus_tag="VLA60_04690"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HSF08686.1"
                     /translation="MSKSLKKNSLPLTEGPDRAPARAMLRAVGLTDDDFTRPLIAIAN
                     TWSEITPCNYHLRDLAASVKQGIREAGGTPIEFNTIVVSDGISMGTEGMKASLISREV
                     VADSIELVVRGHLFDGVIALSGCDKTIPGCVMALARLNLPSIMLYGGSIMPGEFHGKP
                     VSIQDVFEAVGSHAKGKMTTEELIELERRACPGAGACGGQFTANTMSIAFEFLGISPM
                     GFNGVPALDPRKHQVAKECGKILINLLKNDLRPRDIITRPALENAIAAIATTGGSTNG
                     VLHLLALAHEMSLPLNIDDFDTINRQVPLLADLKPGGQFMAADLYQAGGTPLVAKWLL
                     ESGHLHGDQITVTGRTLAEEAATAKETPGQTVLFPPSHPIKPTGGLVILKGNLAPEGC
                     VVKVAGHAKLAHHGPAKVFDCEEDAFQAVQQGKIVDGDVVVIRYEGPQGGPGMREMLG
                     VTSAIVGAGLGQSVALLTDGRFSGATYGFMAGHVAPEAAKGGPIAAVQNGDLIHIDIT
                     ARRLDVELSEQEIQGRLAKWTPPAPRYTTGVLAKYARMVSSASLGAVTS"
     gene            complement(15040..16008)
                     /locus_tag="VLA60_04695"
     CDS             complement(15040..16008)
                     /locus_tag="VLA60_04695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF08687.1"
                     /translation="MTSISLSILIMNKPLCTSQLPSLKRKMTSNLPRSLRYYQKGILH
                     SEAWFSIVENDYTKILGAVNWDSLFPEQNAGYQVLDIGCGPGRFPRLLQAWLPSTIRL
                     HYDYFDPSHYCLSTCGQSLRLPFLPRHAWQTTWDNAEKFLTPGSYDVAWAIQSFSCLD
                     HAALHTSLSRLIGTLHPSRGTACIVLAKQEAFFSQLHHTFFQECSPHAPMPYLSAESV
                     VAVLEKLEAFITIREIACTHSISIRHDRLLEQYLQQVVLDSTPLPNWMKHPRLRQLVE
                     SCRHGDTYHFTNPYWLILCTPASAGAGGRLRLQQYLTSVTSCKLAS"
     gene            16415..17017
                     /locus_tag="VLA60_04700"
     CDS             16415..17017
                     /locus_tag="VLA60_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08688.1"
                     /translation="MKPIQKEKINVRLAYTYKPWVPGHLFFADREAASSPQPSPNFSS
                     KRTGSTLVGSVMALLATFESANVLPPEGTSQANQLIHGLIQMQSALVKSQSSELRDYF
                     SAAVEQRFEKESAGLLQSIHQSGLTSKLLEALVSYNHKIPMWEKPALIKLFQRYNISR
                     PDWELIEKIFGEAEVAYRMKGSSIHDAYELWRAQMPGGRS"
     gene            complement(17029..17997)
                     /locus_tag="VLA60_04705"
     CDS             complement(17029..17997)
                     /locus_tag="VLA60_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSF08689.1"
                     /translation="MKPILLGLIGVGRHGYRYLHHLLSVETGGKLIAISRRNMEEGIR
                     LGTEYSLRFYPNYQDLLADSAIQAVLIVTPPSLNLPIALDAIQLGKAVLLEKPLALNQ
                     IQGRQIVEAATKARIPVMTAQTLRYEPTIRQLQAIASSLGQWHYLVCTMRFEWRQGLP
                     EKNTSWSNYGALMEFGIHLLDLVRVLTQDEIHSVSADISRPTAQDPENRAFIKLVTQR
                     GLPCYLDISRVSQGRVTRAEIMASKGQVLADWTTNIVRKISGRNEILDYPCPSSATLI
                     EVLRDFCQAIRTGGPMPVTAEDGLRAVEIADACYRSAQTGEPVFLN"
BASE COUNT         4701 a         3703 c         4726 g         4938 t
ORIGIN      
        1 gccagaccag ccttcgtcca atccgacatt aggtcgaaga atgtttcttc gacagtcggc
       61 catttccata ggcgccacag ttcatgaatt cgtaaaacat cgtgatgcgc cttccccgat
      121 aaaaaaggag cagcctgttc cagagaaaac gggttggctc cgtcccccag gagctgtgga
      181 tgaggagtta tttttggagc gctgcactgg atgcgctgat tgcgtcgagg tctgtcctca
      241 cgatgcaatt caggtgttat tgagtaacga tactcccgtt atctatccag gagaaatccc
      301 atgcaaatta tgtgaagatc ttccttgtat tggggcgtgt actacggagg cccttttgcc
      361 cgttgcggat cggtttcagg taaatatggg agtggcgaaa gttttttcga aaatatgtac
      421 ggcagaagct ggctgcaatg cgtgtgtttc gaaatgtcct acggaggcgc tctcgatgga
      481 ctttgcctcc tatcacatac gtgtagatga ttcggtatgc gtgggttgtg gcatatgcca
      541 atatatctgt ggaacggtaa atgaccgtag cgcaattcgg attgtttcta gataaggatc
      601 gtgtggtatt ttacgctaag tggtgttgat agcttgaatg atctggagaa gaaaagtgta
      661 aatatggacc ctttagctgg gaggtatgga aaatggacgg aggcataaaa gtggattttt
      721 ctgtgtgtat tttctagggt aaatttaggg gattcaaatt gctggaaaat tctggaaatc
      781 gtggggctat cagggatatt gaagtcttga cttcataggg ctgttgtcct attatctgat
      841 ccctttttca tgaatcctta atggccctga caatgatcct aattgaagca aatgctcgag
      901 aaatgaaaaa ttttaaaagt taggtgaaat atgacatcag gcgttaaaga ggggaaaaaa
      961 gcaggaaaaa acggaatgcc agtttctgga aatgaaaagg ccgtcaagat ttctccaaaa
     1021 gatgcgccag ctgttgatcg ggcactttgt cgaagcaaac tttatctgct ggtgtcctgg
     1081 gggtatctct acccagaaga cgaggagttc ctggattacc tccagagtgg ggaatttgtt
     1141 gaagatggaa aaaccgcatt agaagggttg aaaaaagaac tacaaaatat agggggagat
     1201 gaagcacaag agcgtcttga tgctttggtt ggtaattttg atgcgattga agcttggatt
     1261 tcatcggaag gggaaaactg gagtctccaa gacttacgtg atgagcatcg tcgagtgttt
     1321 agcaatgtca tcgcgttgga ttgtccccca tacgagacct tatttggaaa tgatcatgtt
     1381 ttcggccagt cctacgtaat gggagacatt gccggttttt atagtgcctt tggccttcag
     1441 ctttctccgg atattcatga acgcctggat catttgagtg tagagttgga atttatgcat
     1501 tatctggcgt acaaagaatc atatgcgatt cttcatgatg gagcagaaaa actaaaaacc
     1561 gtcattgaag cggagaaaaa attcgttaaa gagcatattg gtcgatgggt ccctttgttt
     1621 tcaggaatgt taaatagaaa agccgattac ggtttttata agattttggc tgattttacc
     1681 tctcattgga ttgctttcga tattgcattt ttaggggtaa cgcctcaacc ttattcagaa
     1741 acggattatc gtcctgcgtc atacaataac cctgaaggtc aaagctttga atgtggtgct
     1801 caagatcaag gaaatgaact aagcctcctg atgagtgaag ttggggctga tgcatttttg
     1861 gataaagaaa gtggaacatc gggccaatca ggaaatgctt agtgaagcat tgggttaagt
     1921 catttttgaa aatttgtttt agtggcgaca ggtttgagga gacaggtagt tagcagtctt
     1981 ttcggtttta atcttttatc aaaggaggat gcgtcattat gagattggtg cagacgcgta
     2041 acaaacgact ggtgtttggc gttcttctgt ctgtcctggt ggtatgtgta ggtttgacca
     2101 tggggcagat tccgctggcc gtctcgcagc cggtgacaat tccggttggc aaaattactg
     2161 gcccaatccc tatggatgcg gcgaatcctg tatgggagtc cgtgcccggt atcgttgctc
     2221 cattaagtgg gcagacgata acgaccccaa tgcacccgaa catttcagtg aaaactgtga
     2281 tgatcaagat ggcaacaaat ggcgaggaaa tcggcgtttg ggccaactgg ggtgatcaaa
     2341 cattaaataa caccaccatt ggtcctcagg atttcagaga tcaagtggcg gtgcaattcc
     2401 ctgtgcaatc tgcaggcgcg cctccattcc aatgtatggg acagtctggt gggacggtca
     2461 acatatggcg ttggaatgcg gagtggcaaa aagatcttgg agctggcgtg gccggtatgt
     2521 gggatgtgga caatcaatat ccgtccatag catgggatta ctactatgaa gagccagctg
     2581 gtggtgtgac ctatcctgac cgaattggtc ggagcttagg gccatttaac cctggtattt
     2641 ggtctggtaa catcatgtcc gaccctaacc tgcggctggg atcagtcgaa gatttgaatg
     2701 ctaacggatt tagtacgttg actacccaag cgtcccaaga cgtggtagga aatggattat
     2761 gggagccgta tgggtcactg aagggtggat gctgcagcgg tcctacctgg agagtggtaa
     2821 tgaagcggag tctcaagacc caagacccca atgatgttca gttcgcggca ggagcgagct
     2881 tcccagtagc ttttgcggtc tgggacggtt ccaacgtaga acgcaatggg atgaaaggga
     2941 tttcgacttg gtttacggct caaatgccaa actaagttca ttccttttct gagtcaggtc
     3001 cttttttata taggacatta gctaagcctg aaaacctccg gtaatggact ccagtaatgg
     3061 gggaagttgc cggaggtttt tttataagcg catttttccg ggtaaatgtt tatgcgacaa
     3121 ggcaagaaaa ggctaattgt tgcattgaag agaatgggac gtgtataaat ttaaaatagt
     3181 caaattttcc gtttcttatt atccccatta cctgggagcc gcattgtgaa aatttcactt
     3241 ttattccctc caagctggca tccctcccag ccctatttga gtgttccttg tctttcagcc
     3301 tttcttgaaa aatccggtgt tccagttcat cagcaacgtg atttaaatat tgagttgctt
     3361 gatagggtgt taaccaaagg ctttgggtta gatatttacc cacggttagt tgatttggtg
     3421 aaaaaactgg aagtatcggt ttctggagat accggttatg ggagtaagga gcattacgct
     3481 cgtgttgttg aatcacttga ccgatttccc tatctcatag atgaaatcga acccgttaaa
     3541 gcctcattgc gtggggaaga gttttacgac attgagcgtt accgagaatg tctctttttg
     3601 attgatcggt ggttggaggt tgtatcctct ttatatttcc cgaccaggat gacggtggta
     3661 gacaatcaat ttgcatattc aatctattct tcacgagaaa ttcttcaagc tatacacgat
     3721 gaaaaccaaa atccgtatct tgagctttat cgaaaattct ttcttccggc ttttggacaa
     3781 gatatttcaa gctttgttgg agtctctatt accgcaacct cacagattat ccctggttta
     3841 actctgtgta aattaattaa ggaaaatcat cctgaggttc atgtaactgt cgggggtagt
     3901 atttttacaa ggctggtaga caatctcaga cggtgtgatc ggttatttca attgactgat
     3961 gatttcattg tgtttgaagg cgagaccgcc cttttggaat tgattaatca gctagacggg
     4021 aaaaaggatt ttacgaaagt gccgaatctt atctggcggc aagatggtaa aataattgtg
     4081 aatcaaccct tctactctga aaaccttgcg gaccacccag ctccaaatta tgacggtttt
     4141 cctttgagtg actatttagc cccccatacg gtgttgccgg tccaattttc tagaggttgt
     4201 tattacaagg attgtgcttt ttgtgccctg actttggatc atcagaattt tcggcaaaag
     4261 gatccgataa aagttgttga tgaattggaa gcgttgtccc ataaatatga aacaccgtat
     4321 tttttcttta cggatgaatg cctggccctt tcgccaacca agcgattgtg taaggaactc
     4381 attcatcgag ggttgggttt acagtggacg gctgagttgc gatttgaaaa gaatttatct
     4441 cgtgagttac tcacctccat gcgggaggca ggatgtcaga aaattgtctt tggcttggaa
     4501 acctataatg cccggttgat ggattttatg gtgaagggga ttacccaaga aaatgttgat
     4561 cggatctgtt ccgattgtgt tgatcttgga attgcggtgc attgctatgt gatcgtgggg
     4621 tttccgaccg aaacagaaga agaatcattg gaaacattga attttattgt caataatacg
     4681 aagctgcatt cctcctatgg gttttcctgt caaccgtgtt tgtttgattt agaaaaggaa
     4741 gcccctatta tgagcgatcc agggggatat gggattcaac gaattatgcg accagcctcg
     4801 gaagatttga gcttagggtt tttttatgaa gtaaaatcgg gaatgactcc agagcaggct
     4861 gaaaaagtat accaatatgt gtatgaaaag attagcgaag ttgtgtgtga attacccttt
     4921 aattactcca tggccgatgg gttgttatat atcacccggc ataacaaaga gcttaaggtg
     4981 gagccctcca gggtgtaaat aggaagatat cctggcctgg atgaaagtct agttatctta
     5041 ttacgcatgc gcatcaatat ctgaagttct aaaaaaatga tttctactac tgcaaagaac
     5101 caggaaagaa tcgacggccc ggggcttctt tttgaatggg gtctcaagag tttgctgttt
     5161 gcggcctttt ttgaattggt actttatcgg ctggtttcac ggttgggtat gcaccttggc
     5221 aaattggctg aaaaatatga agcggtcagg atcggatttc gcacactctc ttcattaggg
     5281 tttgtcctgt tgaatatgac ggccattctg gtttttctga ttttgggtat tttgctggtt
     5341 caaaaattac ggacaaagct atgggtagga gagtgggata aggttgttat tccattggcg
     5401 gccttattgg tagcttgtac ggtgggttat ttattttttc ctccggtcat ggtcgggacc
     5461 attgtttata atattttggc gttcgggttg atcgtttttc tggttttgga atattgggct
     5521 actcaccggc tgttgagtca acggcttatg gtaggtagtt ttttcctggg tattagcgga
     5581 tggctgtatt atcagacgat gtcaacgact tatggatggc tcaatatttt tgatgcccct
     5641 ccgctggtgc atgaagggaa taggctcggg gaagcactaa tggttctttc aagcatcctg
     5701 gtgttttggg cctatgggac cacttcaatt attaccaaaa acaagcaaca acgccggtgg
     5761 ggattaactt tggttattag cgggggcagt gcttttgtgg ccttactgtt catggatttt
     5821 ttcctttctc tttggaatcc tgaagtggcg aaagaagtca ggaaggctgg agaggggata
     5881 ggctggattt ttcaaatggg aatgggctat accttttatc tcccttttgc attttatgtg
     5941 ttgggcttta cctgctgggc gtataccgtg attaagttgg tgttgattgg acgattagca
     6001 ggatatgggc taggtctcat gtttatggca ggctatgcgc tccaattgtc acatttgacc
     6061 ttgatggtag tgcttgggtt aatgttgcta aatattgata aaagaggaac tttagggaaa
     6121 cttcgtaaga aaacccaaga gggggtagtc tatcaaccaa ttcgtcccgt ttacgggcaa
     6181 acacagtagt taggacacat gaaatggaag aacaaggtgc tggaagccaa acaatggatc
     6241 cgcagtcggg ggagacgacc acagaagaac gccccttaac ggttgatgag gaaattgctg
     6301 agattgaaaa gttactgagc gaggagccgg acgactttca agcgcggtgt cgattgggtg
     6361 aactttattt caataaagga cggatggatg aagccctggc cgaagtcaag aaatccattg
     6421 agatggcaga gggaattcgg attgaaatgg atcggtctct ggccatgtac tactcgaatc
     6481 ttggaacaat ctatggaacg aaagggatgt tggatgaggc cacggctcaa tttaaacgag
     6541 cgctggaact aaacccctat gatgttctgg cactctttaa tgtcggccga ctctataatg
     6601 acaaaaatgc atttctggag gccaaagagt atttcgaacg tctgatcgaa atttcacctg
     6661 aagatccgat tgcttggtat aatttggctg gagtctatga gaaattggat gatcccaacg
     6721 tctccgactt taatacgata gacatggcta tgcaggctta tatgaaagtc ctgcagtttg
     6781 atccaagtca tcttgaatcg agttttaagc tcatggaaat tgccctaaat ctgaagaaac
     6841 ctgatatggc agtaaagatc atggaagatg ccgttgaaaa taatcaggat gaaccattgg
     6901 cctattataa tctgataaac acctatgaaa agtgtaagat gtttgagcag gcggaagaaa
     6961 ccagaaatcg tctcaaggat cgatttaaca agcgttcgcg tgaaacggta aaaaagtgat
     7021 accccaacaa ggagaggtac catgtttgga agcttaggat ttacagaatt aattctcatc
     7081 ctcatgattg tgctgatcat ttttggggcg ggaaaattac cgcaattggg cgaaggggtc
     7141 ggaaaagcga ttaaagggtt caagaaatct gttcaagagg cagaagcgta tgacctagac
     7201 ccccaggcac aggcggaaaa tacagcatcg gccaaccagg gtaatgctca accgcaaata
     7261 tctcatccta tgcatactgc cccgttagaa acggtttccc cagtgccacc ggcagcagtt
     7321 cagccggctc aacaggaaca ggttcctgcg tcatctaagg cctaattctg ctaaaccggc
     7381 aagggggcca taatcttttt ggtggtgatt ggacaggatt ttttgaaata aacgttgaag
     7441 ggtgtaattg tctatgagta ccgagaccgc gttagaaatc ggggtgattg aaacctttgc
     7501 cgggaatgga aaatctcgca gtacgggtga tggcaagcga gcagtgaaat ctggaattcc
     7561 actgccaaat catattacgg ttgatcggca tggtcggttt ttctacattg ccgaatccgg
     7621 ttctgaccga atcaggcgag tggatatgga aacaggcctt ctgcataact ttgcgggtac
     7681 cggggagaca tgttatagcg gcgattacgg accttgtggg gaagctgggc tttaccttcc
     7741 attagatgtc ttgtgtgatt cgcacaatca tatctatgtt tgtgattcag gcagcaatcg
     7801 aattcgaaaa attgacgcgg atacaggaat cattacgacc atcgtgggga cggggcagtg
     7861 gggatataat ggcgatgggc cggccttgga gtgcaattta acctatccgg cagccatagc
     7921 aatcgacgat caggatcatc tttacattgc agacactcaa gcccatcgca ttcggcatta
     7981 taatcctcag acaggaatgc ttacgaccat tgccgggtgt tggacggcag aagatgatga
     8041 gcgcgtgagt cctctgaccg cacaaaatct aattgtcctt tctggtgatg caattgggat
     8101 agattttagc aacgatgaag ggttactgcg tccgcgttgt tcagatggac tagatttatc
     8161 gttatatctt gacgatggca agccggcttt ggagtgcaag ttgtatgacg tggtcgatat
     8221 cgatgtggac aaacacggcg atctttatct cactgataag ggaacaaacc gaatccgaaa
     8281 gctagatcgg aaaacgggta cgatgtccac cgtggctggc atctgccggt atgggtttga
     8341 cggggatgga aagttagcgg tgaaatccat gcttaatgtc cctgaggggg ctgtggtgga
     8401 ctcagatgga aatgtgtata tttccgattc catgaatcac cgggttcgta aagttgattg
     8461 ccagaccggt atcattacga ccatcgctgg gaatggcgat agtggctacg atgacaaaaa
     8521 tatgggtggg tgcggtgctg cgcgctttgt ggcgaaagaa gacgctggga tgttgaaaca
     8581 tggtgatggc cttattgcca ttgaggcgat tgtgaacact ccagctggcc tgaccttgga
     8641 ctcacagggg tatttgtata tttgtgaacg gaatgaaaat aaaattcgtc gggtgaaatt
     8701 acgataatga ggctaatttg atttttcaat agagattagc tgtatccagt acagctactc
     8761 ctgccccagt gagataaata attgctgcca catttattag ttccaagcta tatcatcatt
     8821 ggtttcgctt ccctcctcat aaccttttgg tgggactccg tcatttctcg ttgctcattt
     8881 ttgttcaaaa tgcctgaaag cgtcgttccg gtttgtttta cgcgctgctc tgtcttcaag
     8941 atgggatagg gctgtaattt gaaaaatatg catacacata gacaatgatc atgaatctgt
     9001 tcgcgagtat ctatgaaata atcaactgat ttttctcatt cttgaaatat cgttggcatt
     9061 gaatctttaa aaattccaag gaacccgagg attgagcaca attcacgccc caaaacggat
     9121 tgttttgttt gtggtttttg cgttggtggt tttattcggg ttgacttggt atcaagtccc
     9181 acttgtcagt tctcagggca tggttgtcct ggcgcatttt tcaaaagggg aagtgccctc
     9241 tcatccttcc gattcagcgt gggagaccgt ggctccgctc cctgttcccc tcagtggcca
     9301 aatcattacc agaccggtat ggccggagcc cagtgcgaga gctctctcta tccgatcaat
     9361 tcataacgga aaagacattg cgtttttgct ggaatggcag gatgccacaa ttaatgaatc
     9421 gttgacgcct ggggtcttca gggatggggt tgccgtggct ctgcctctgg gagatgcacc
     9481 ggcctttttt tgcatgggcc aacttgatca ttatgtgaat atttggcatt ggaaagcgga
     9541 ttggcaaagc gacgtggatc ggcgtgccga gcgagccaaa gaatctaagc gtggggaatc
     9601 aggtcctcgc cggtttgaag tgatcccgcg tcggccctca tcggtcgagg acctcgtggg
     9661 aggtgggttt agtaccctga ctagcaaaga acagcagggg cgaattcagg gacaagcgga
     9721 gtggaaaaat ggcctatggc gagtggtcat gaaacggcct ctgacgtcta tgggagacga
     9781 tttggacaat gaagcgatgc tggttcccgg gcgaatgcaa gccatagcct ttgccgtttg
     9841 gaatggagaa aataaggagc ggaatgggca aaaatctgtt gcctcctgga tgcaattact
     9901 tattgatcct gttcagcccg catctccaaa tggggcaggt ggtattgaat ccacgcatag
     9961 ctcatcatga gggtcccaat aatgaagagg atgagatcta cagggggaat gcaatggttc
    10021 ttaatatgtt ggtgcctgtg gtttggattg atggttcttc aaggagaagc gtttggtcaa
    10081 ccgccaagtg agaatatgac caatgaaaaa gccctgattt tagccgaaaa atttggcatc
    10141 gatgtggggg aagtagatga ggaggtcaag aaaattcttg gattgaccaa agctgaaggg
    10201 gttgtagtgt atgccgtcat aggagggtct ccggctgaat tgtcaggcat aaaggtgaaa
    10261 gcgattatta aggaagtgga taaatatgaa attaaaaccc ttgtagattt gggaacagcc
    10321 ttggagcaga ccctccccac aaagaatttt actgttgcca catatgaacc tgcggatcct
    10381 gataatcaag gggttaccgg cgggctaaat ttccattttg tacgaattct tcaggattaa
    10441 atttcttgca tgaagaatct gtttagcaaa aattttcggt tattggaaat aatacccatg
    10501 cacaaccgat ggattttatc gatcatatta gggatttttt gcttatctga cgtcgggttt
    10561 ggtcaacagt caagcactgt tgttggtgaa gcagaagagt cctggattaa ggaaattgag
    10621 caagttttta tcccttctga acaatgcaaa caatgccatg accggcatta tgaagagtgg
    10681 aaggggatgc gcgagcagac catggattta aagacatttg gtcgcgtgga tggagctctt
    10741 cttcatggga cggctttcac ctctccggtg ttcaaaaccg ttttaggtct gtggcttcac
    10801 acggatccgg atcaggagca acgtactcgc tgtctttctt gtcatgctcc agcggtgacc
    10861 gtgttccctc agcacactga tcggattatt gatcaagtga tgaaagggga aaaacgtgtc
    10921 aaaattgaag gcatcggttg tagtgcctgc cacctcatca cgggcattca agagaatgcc
    10981 tatggtcacc ccacttttaa aatcacgtct gggaagactc tttacggacc ctacgcagaa
    11041 ccagaggaga atcttgtgca tccatctggg caggtggata tttatcgagg ggcccattat
    11101 tgtgcatcct gccattttga taaggtaagg gatgttactc ggcctgatat tccaggggaa
    11161 attttgaaag gcaccatttg tcaggattgt catatggagc gctctacggg gagttcaacc
    11221 tctcaacgtg gagctctcac tcggccaatc gggcggcatt ggttccaggg tattgtgatt
    11281 cccgggatta tgctgagtaa tagaaattta caggccgaat ggttttcgag ggtggatatt
    11341 gaggttgaaa aggttaaggg gcaggttgag ggtgaagtcc tggtgcgaaa tggtgccttg
    11401 cctcatactt ttccgggtgg cgatccagtg ttaaaacaat tttatgtcac gataactctc
    11461 aaaaattcag atggacaggt tatcgatcaa tatcaggaac ggtttggccg aacatttgaa
    11521 gagctgcttc gtggtccgat ccctagacca tttgtcaatg gaggcacaac cagacatatc
    11581 ccatttacgt tgaaatatcc acaagattca gaggcttccc tcattgaagc ctcagtcagt
    11641 tattctctca ttcctgagcc ctcgaaggca cttgccgcga agttccttga aagcctggat
    11701 accgataaag aacgggaagc tgcagaacgc attattcatg attattcttc cccgcgtctc
    11761 ctcacgtttc gcaccatgag tttataggca gcaggaacgt tcagattttt tgttgaataa
    11821 cataaaagga ttgtctgatg aggaaaagct acaaaaagag tgcccccaca ggtatgaggg
    11881 gtggggtatt tctgctggtt ttgggatgct ttctgctgac ggcacctctt cctggctcgg
    11941 ttgcaggtga atcgtcttca accaaaaaac cattagaaaa atcatttccc aattccgaaa
    12001 aatgtaaacg ctgccacctt agggtcttcg aagaatggga agcttcggct caatcgcgtt
    12061 ctattgtgac tgcccctttc cgagtaacct tggaccagtt tctggcttca accgataaaa
    12121 aagatcatgc aatgtgcttt cgttgccatg ctccgcacat tcttgaatat gccgaccact
    12181 tgcctcgttt cattaaggaa gtgcaatcaa aggacccaca gatggatgga gtcggatgcc
    12241 cccaatgcca tcttattcag gatgtggaca tgaattccca tcccccaacg ccaacatttc
    12301 aattggggac gacgattttt ggaggatatg ataaagccgc tgagaacctg gctcatcaat
    12361 cacagaagct gaatctctat cgagagtcaa aattctgcgt gacctgtcat gattcacttc
    12421 caaagatctc aggctctgcc aaggacctcc ctggttggct tggatcttgg gaaaaaacca
    12481 aatctgaaac caatgggaag ccttgtcaat cctgtcatat gccagaggca gttgatgaat
    12541 ctgctaatgg ggagaaggtc aggaaggtcg ccaatcacag tttccctggg agattcggaa
    12601 aagtccgcgc ggaagctgtt accttggatt ttactactga gaccactcct gagacttctc
    12661 aagtgcaggt cagtattcag agcctggttc cccataattt gcctttgccg catcccgggt
    12721 ggtctcgggt cgtcgttgat ctttctattt ttgggaaaaa tctcaaaaag gtttacaacg
    12781 aacaacgttt ttatgagcgg acgtttggga atggtgagga aaaagagacg gtctttgatt
    12841 ttgaggcaaa aaaggtatta cgcgacaccc ttcttaagcc tgaggagatg cgggtagagg
    12901 tttttacatt tcccacacca caggatgcac catcaatgga tgtagtggtg actctcacat
    12961 atgcccctgt acacggacca gaggatttcc ttaaaaaggt tgaacaagaa gcggcacttg
    13021 ggcaaaagga caaggccttt caatcggtcc aaattgccca aaagaagtcg aacgttcctc
    13081 ttaaaaaata attactgctc cctggagggt gacccgttaa gaagttacgg ctccgaggga
    13141 ggccgaggaa accatcctgg catatttggc caggacaccg gttgtatatc gtggtgctgg
    13201 tggagtccat tttgccaaac ggccttgaat ctcttgctct gaaagttcaa catccaaccg
    13261 tcttgcagta atgtcaatat gaatcaggtc tccgttttga accgccgcaa tcggtccccc
    13321 ttttgcggct tcaggggcga cgtgtccagc catgaagcca tacgtggctc ctgaaaaccg
    13381 tccgtccgtc aaaagagcga ctgattggcc taatcctgct cccacaatag cactcgtcac
    13441 ccctaacatc tctcgcatcc ctggtccgcc ttgtggccct tcatagcgga taacgacgac
    13501 atccccatcc acgatttttc cttgttggac cgcctgaaac gcatcttctt cgcagtcgaa
    13561 gactttggcc gggccatggt gtgccaattt ggcatgccca gccactttga cgacacagcc
    13621 ttctggggca aggttgcctt tcagaatcac aagtcctccc gttggtttaa ttggatggga
    13681 agggggaaaa agaactgttt gccctggagt ttccttggct gtggcagctt cttcagctaa
    13741 cgttcgtccg gtgacggtga tttggtctcc atggagatgg ccagattcta aaagccattt
    13801 tgccaccaac ggtgtgcctc ctgcttgata gaggtcggct gccatgaact gacctcctgg
    13861 tttcagatca gccaggagcg ggacttggcg gttaatggta tcaaaatcgt caatatttaa
    13921 tgggagcgac atttcatggg ctagggctaa aagatggagg acgccattgg tggatccacc
    13981 ggtagtggca attgccgcga tggcgttttc caatgcgggg cgagtaataa tatcccgtgg
    14041 gcgaagatcg tttttcagga gattgatcag gatttttccg cattccttgg ccacttggtg
    14101 ctttctgggg tcgagggctg gcacgccatt gaatcccatt ggggaaattc ctaaaaactc
    14161 aaacgcaatt gacatggtat tggccgtaaa ttgaccaccg caggcgccgg ctcccgggca
    14221 ggctcgtcgt tctaattcaa tcagctcctc cgttgtcatt ttccccttgg cgtgtgatcc
    14281 gacggcctca aagacgtcct ggatcgacac cggttttcca tggaattctc caggcataat
    14341 ggatcctcca tagagcatga ttgatggaag attcaggcgg gccagcgcca tcacgcatcc
    14401 tggaatggtt ttgtcgcaac ccgataaggc aatgactccg tcaaacagat gcccgcgaac
    14461 cactaattcg atagaatccg ccaccacttc tcggctaatc aaggaggctt tcatcccctc
    14521 cgtccccatg ctgataccgt ctgagaccac gatcgtgttg aattcaatgg gggtccctcc
    14581 cgcctcacga attccttgtt tcacactcgc cgcgagatcg cggagatgat aattacaagg
    14641 ggtgatttcc gaccaggtat tggcgatcgc gatgaggggc ctggtaaagt cgtcgtcggt
    14701 caagccgaca gccctgagca tcgcacgagc gggggcacgg tccggtccct cggtcagggg
    14761 gaggctattt ttcttcaagc tttttgacat gaaacagtgc tccttcgatc ataaccgttg
    14821 atgaatggaa tataagggca gtcgatgatt cgtttcctga atgtctcata atgctaggaa
    14881 aatggtcaat gttggtggga caaaagattg cacgtgcaac cctggcgatg atgattttcc
    14941 tgggaatcag caggagaaga gaaatggccc cgcaggggag agacgccaca cagtcttgtc
    15001 cactgacatg gcaaggatgg tggtttgctc gtgagattcc taggaggcga gcttacaaga
    15061 tgttaccgat gtgagatatt gctggagccg aagcctccct ccggctcctg cggatgcggg
    15121 tgtacacaat atgagccaat atggattggt aaaatgatag gtgtctccat gacggcagga
    15181 ctctacaagc tggcgtaatc tggggtgctt catccagttt ggcagggggg ttgaatccag
    15241 gacgacttgt tggaggtatt gttctaacag tcgatcatgc cggattgaaa tgctatgggt
    15301 gcaggctatt tctcgaatag tgataaaagc ttctaatttt tctaaaacag caactacgga
    15361 ctctgcagaa agatagggca ttggagcgtg aggggaacat tcttgaaaga aagtgtgatg
    15421 cagttgtgag aaaaaagcct cttgcttggc aaggacaata catgcggttc ctcgagatgg
    15481 gtgcagagta ccgataagtc gggacaatga agtatggaga gcggcatgat ccaggcagga
    15541 gaaggattgg atcgcccatg ccacatcgta agaacctgga gtcaggaact tttcagcatt
    15601 atcccaggtg gtttgccagg catgtctggg gagaaatggc aggcgcagag actggccgca
    15661 ggtggagagg caatagtggg aagggtcaaa ataatcataa tgaaggcgta tggttgacgg
    15721 aagccatgcc tgcaacaatc tcggaaatcg gccgggtcca catccaatgt ctagtacttg
    15781 atagcctgcg ttctgttccg gaaatagcga atcccaattc acagcaccca agattttggt
    15841 gtaatcgttt tccacaattg aaaaccaggc ttcggagtgt aagatcccct tttggtaata
    15901 acgaagtgat cggggaaggt ttgaggtcat ttttcgcttg agagaaggca attgagaggt
    15961 gcataagggt ttgttcatga ttaagatact gagagaaatt gaagtcatca gttgttccta
    16021 gaagcaaaaa tggtaccggc tatcatttgg taaaaagagg aaagaagaca atgggatttt
    16081 atcaaattgt ctgttaggaa gggggaaggt ctttttcctg aagcaaaaaa attccccaac
    16141 acacaggaag aatctgccgg ttgcctattg gactcatatt aacggtcatt tgaccataca
    16201 tcggttttgc tgaaaacgtg aagacagcaa ggaaatggtt tccaaagaac cagtaaaggg
    16261 catgaatctg atgatctgcc agggtgacac tcgtaaatcc tcgtggatct tcttgactgt
    16321 atggataggg gcgactgtct tgttcctgtg ggaggttggt ctaagtagaa gtgagggagg
    16381 tttggagaat gagtcctttt ccgaagttgg aaatttgaag ccaattcaga aggagaagat
    16441 aaacgttcgc ttagcctata cgtataaacc gtgggtgccg ggtcatttat tttttgctga
    16501 ccgcgaggct gcctcgtctc cacaaccttc gccaaatttt tcttcaaaga gaactggctc
    16561 gaccttggtt ggttccgtca tggccctctt agccacgttt gaaagtgcta atgttcttcc
    16621 accggagggg acctcccagg ccaatcaact gatccatggc cttattcaaa tgcagtcggc
    16681 cttagtgaaa tctcaatcct cagagttgag ggattatttc tcagcggcgg ttgaacagcg
    16741 ttttgaaaag gaaagtgcag ggcttttaca gtcaattcat cagagtggtt tgacatccaa
    16801 attgttagag gccctcgtga gctataacca caaaatacca atgtgggaga agccagctct
    16861 tattaaactc tttcaacgct ataatatttc ccgaccggat tgggaattga ttgaaaaaat
    16921 attcggcgaa gccgaagtag cctatcgtat gaaaggatca tccatccatg atgcctatga
    16981 gctttggaga gcacaaatgc caggaggccg gtcatgaaga tacagtaatt aattgaggaa
    17041 aacgggttcc ccggtctgtg ccgatcggta acatgcatcg gcaatttcga ctgctcgtaa
    17101 cccatcttct gcggtaacgg gcattgggcc tccggttcga atcgcctgac aaaagtctcg
    17161 aagcacttca atcaacgttg cactggaagg gcaggggtag tctaatatct catttcttcc
    17221 ggaaattttt cgaacgatgt tggtcgtcca atcggctaag acttgtcctt ttgacgccat
    17281 aatttctgcg cgggtgactc ggccctggct gacgcgtgaa atatcgagat aacagggaag
    17341 tcctctttgc gtaaccagtt tgatgaaagc ccgattttcg gggtcctggg ctgttggtcg
    17401 actaatatca gccgagaccg aatggatctc atcctgagtg aggacgcgga ccagatccag
    17461 caagtgaatc ccaaactcca tgagggcccc ataattgctc catgatgtgt ttttttcagg
    17521 caacccttgg cgccattcga atcgcatagt gcaaacaaga tagtgccatt ggcctaagga
    17581 tgaggcgatg gcttgcaatt gccggattgt tggctcatac cgcagagtct gggcagtcat
    17641 tacagggatt ctagccttgg tggcggcttc aacgatttgg cggccttgga tctggtttag
    17701 ggcgaggggt ttttccagaa gaaccgcttt gccaagttga atggcatcta aggcaatggg
    17761 aagattgagt gagggagggg tcacgatcaa aacggcctga atagccgaat cggctaaaag
    17821 atcctgatag ttcgggtaaa agcggaggga gtattcagtg ccaagtcgaa tgccttcttc
    17881 catgttcctt cggctgatgg caatcaattt tcctcccgtc tcgacggaga gcaaatggtg
    17941 aagataacgg tagccgtgtc gtcctacgcc aatcaaccca aggagaatcg gtttcataac
    18001 aaccctggtt ctttccctta accccaagga gtgataacaa aagagaagtt gttctcatcc
    18061 aatatgta
//
LOCUS       DATIAL010000148         8125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_886165, whole genome shotgun sequence.
ACCESSION   DATIAL010000148 DATIAL010000000
VERSION     DATIAL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886165"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..334)
                     /locus_tag="VLA60_04710"
     CDS             complement(53..334)
                     /locus_tag="VLA60_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF08690.1"
                     /translation="MLTGFGTYYDKIPDEQLSSAIKERFRLDGRINADQIVIEVKSGH
                     VMLSGVVDTVTEKSLAEGLVAGSIIRVKSVVNNISVRPAVSQDDAIKQQ"
     gene            complement(456..1457)
                     /locus_tag="VLA60_04715"
     CDS             complement(456..1457)
                     /locus_tag="VLA60_04715"
                     /inference="COORDINATES: protein motif:HMM:NF022391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HSF08691.1"
                     /translation="MNLHHLKSLLIGALVLGISLGQHLWANEPTKKQITNGSTTHEEV
                     ASSVQSEVESTGKEEVVKKRKALVKEALNALSGTKKALKALEDGNTKDALAALAEVTG
                     QLEIIISRDPHLAFVPVDVEVTSTDIYADLDSIKKAKDQVEDFLGNGEVQQARVLLSG
                     LASELVVSTISLPLATYPDAVKAVVPLIDQGQIEKAKAALQSILQTLVVTNERVVPLP
                     ILRAEAFIKKADDLAKQIESPALKNPTTRDEKDEVKISENEKFQKEQILEQLQNARRQ
                     LKMAEALGYGLKEDRYKEFHESIEAIEKKVQGEESPQGIFASLKRSVSQFKQFLFEE"
     gene            complement(1752..2135)
                     /locus_tag="VLA60_04720"
     CDS             complement(1752..2135)
                     /locus_tag="VLA60_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08692.1"
                     /translation="MNGSVDRAYGPYAHHYQQFFEHCSGKKGYNFGEAPRINMPSPLV
                     RFPRLKWWSWDRPKRQRKIRLVRDQSLMEILRLGDLAKTFPHGEISGDQHTGKRMAQL
                     SDKHARARCDGSRDSAYHYNNPRAA"
     gene            2959..4488
                     /locus_tag="VLA60_04725"
     CDS             2959..4488
                     /locus_tag="VLA60_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSF08693.1"
                     /translation="MSQSQLSQRWNGRRVATFTFIGIGLLIAGICLGLGIAADSSLLP
                     GLSPQLKVSGTPLSDSSSQPSGALSFGQQDAQGQSTNLIQVAKLARPAVVNISTTATA
                     KTPEPLRSPFFDDPFFRRFFGDEFERRFEPPPSPRQEGGGSGVIVSDDGYIITNNHVI
                     ENSDAIQVLLSDKRTFTAKVIGTDPKTDLALLKIDAKDLPVLSWGDSQQLQVGEIVMA
                     VGNPFGLSQTVTMGIVSAVGRANVGIVDYEDFIQTDAAINPGNSGGALVNLQGELIGI
                     NTAIFSRSGGYMGIGFAIPSNMAKSIQASLIQHGKVVRGWLGVSIQDLTPDLQTQFDT
                     PDTQGALVSEVMEDSPAKKAGIQRGDIIRKYDSQTVADTRHLRSLVAESVPNSPVTLH
                     VLRDGNPREVQVTLSEMPKDVRALASTGELRGHHALSGITAESMPLGEKGVKVTKVDP
                     ESTAFRAGIREGDIILEINREAVNNADDFQRLTGKLGSKDRVLLLLQRGRSTIFFSVT
                     P"
     gene            4798..5757
                     /locus_tag="VLA60_04730"
     CDS             4798..5757
                     /locus_tag="VLA60_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSF08694.1"
                     /translation="MASAQRDLYDILGVKKTVDAKELKKAYRRLARQYHPDLHPGEKK
                     TEMEKKFKELNEAYEVLGDEENRKKYDQYGLNWKEAEAYERARQQGGGAYPGGGPGGF
                     TQGGADFSDIFESMFRQGAQREGAGFRGFAMAGADLEANLPISLREAFTGTRRALNLS
                     DAGGTPRSIEVRIPAGVRDGERLRVKGKGAPGRGGGPPGDLFFHVQIAPHPVFQRKDS
                     DIVVTLPLWPWEAVLGTDIPVPTLSGTVRLKIPAGSQSQQRMRLKGKGLPKRTGGHGD
                     QFVVLDIVTPETLSSEEQQLYEQLAKFDHPDPRSSLLREAAND"
     gene            5750..6076
                     /locus_tag="VLA60_04735"
     CDS             5750..6076
                     /locus_tag="VLA60_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HSF08695.1"
                     /translation="MTDDSEPSLEQVTAEIITFGRIRREEVCTRLGIGEDMLEVCLQW
                     EIIPPPEPDPEGMVLFPEDAIDRLSRGLRLHRDLGINWPGVSVALELLDRIEELEQQI
                     HNLSSQ"
     gene            6514..6675
                     /locus_tag="VLA60_04740"
     CDS             6514..6675
                     /locus_tag="VLA60_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08696.1"
                     /translation="MEKPNEIIQQSICTVRQSSEEEALHEVSLHHFHRHPVVSAGGNS
                     RRFPIGRTD"
     gene            7178..7783
                     /locus_tag="VLA60_04745"
     CDS             7178..7783
                     /locus_tag="VLA60_04745"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HSF08697.1"
                     /translation="MMAISKRLGYSTSYKKKELTGDDPYSMKAAPKGFLQCPECQAVY
                     HRKRWSFPGTSSPRSSKSTAGTPKKPTRQVMVPQSFVCPACRKLRDGYAEGYLAIHWP
                     NWGTHKAEVLGLIHNEESQASRNNPLERIMAIRTRPDGADIETTTEHFAQRLGKHLDR
                     AFKGKITYQWSHKDKLARVEWQGPKRPVLPRQKSKTSQTKK"
     gene            7854..>8125
                     /locus_tag="VLA60_04750"
     CDS             7854..>8125
                     /locus_tag="VLA60_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSF08698.1"
                     /translation="MKSRIMVWSLILLIGWAIPTGAQEPQVDPLQGEVVYQAHCVRCH
                     GIQGKGDGPDAAALVVPPANFQRMESRAKSEMDLRSAIIWGLAFSP"
BASE COUNT         2057 a         2078 c         1905 g         2085 t
ORIGIN      
        1 ctcacgttaa ccgatttccc ctgaagggct ggcgtgtttt tcaaatactc gtctattgct
       61 gtttgatcgc atcatcttgg ctcacggccg gtcgaacaga aatattattc acgaccgatt
      121 tcactctgat gatactccca gcaaccaggc cttcagcaag gcttttttct gtcacggtat
      181 ccactacccc actcagcatg acgtgaccac ttttcacctc gatgacaatt tgatccgcgt
      241 tgatccggcc atccaatcta aaacgctctt ttatggccga ggataattgc tcatcaggaa
      301 ttttatcgta ataggtacca aagcccgtca gcaaggaatc ccagtatcat taaacacatc
      361 catgtgtatt tttcacttac ggttcgcttt ttcctctctc ctctcaaatc agttattgtc
      421 attaatctgt gacaatctgg gacgaaagtg tacaattatt cttcgaaaag aaattgctta
      481 aactgagaaa ctgaccgctt caaggaggca aatatccctt gcgggctttc ctcaccttgc
      541 acctttttct caatcgcctc aatcgactcg tgaaattcct tgtaccgatc ctcctttaac
      601 ccgtatccta atgcctcagc catttttaac tggcgtcgag cgttctgaag ttgctccaga
      661 atttgttctt tttgaaattt ttcattttcc gatattttca cctcatcctt ttcatcacgc
      721 gttgtcgggt ttttcaacgc tggggactct atttgtttgg ctaaatcgtc agccttttta
      781 ataaacgctt cagcccgaag gatgggcagt ggaacgacgc gctcattggt cacaactaag
      841 gtttgcaata tggattgaag agccgctttc gccttctcta tttgcccttg atcgatcaag
      901 ggcaccactg ctttaactgc atcagggtat gtggcgagag ggagactgat tgtgctaaca
      961 acgagttcac tcgcaagccc tgaaaggaga actctggctt gctgcacttc cccgttccca
     1021 aggaaatctt ccacttggtc ttttgctttt ttaatactgt caagatcggc atagatgtca
     1081 gtcgaagtca cttcgacatc gacgggaaca aacgccaggt ggggatcacg ggaaataatg
     1141 atttccaact gcccggtcac ttcggccaat gcggccagag catctttagt gttgccatct
     1201 tccaaagcct tcaacgcttt cttggttcct gaaagagcat tgagggcttc tttcaccaaa
     1261 gctttgcgtt tttttacgac ttcttctttt cctgtagatt ccacctctga ttgaacagag
     1321 gaggctacct cttcatgcgt tgtcgatccg ttggtaatct gttttttggt cggttcattc
     1381 gcccataggt gttgccccag acttattccc agcacgagag ctcctattaa tagacttttg
     1441 agatgatgaa gattcatgtc gcctcctttc tgagttgttt ctccatatta ttggacaagc
     1501 cttttcttgg aattgacaca attcctcttt ttgcacggcg tcaaccctct tgaatggttg
     1561 aatggtagat ttgcgaatta ttcaaatcag cgtttccctc acaaaaataa aattaagcat
     1621 gatcattttt ttgtcaagat ccaatgacta tccagcatac cggagcttct aaacggtgga
     1681 catggctttt ccggacgtca tttcaagaat ctgtggcgct gccatgaaat tgaaatggtg
     1741 ccgagatcat gttatgccgc ccttggattg ttgtagtgat atgcggaatc tcttgaaccg
     1801 tcacaccgtg ctctcgcatg tttatccgaa agttgggcca ttcgtttccc cgtatgttga
     1861 tctccagaaa tctctccatg ggggaatgtt tttgctaaat ctcccaaccg caggatttcc
     1921 attagagatt gatcccgtac aagtcgaatc tttctttgtc gtttggggcg atcccaggac
     1981 caccatttga gccggggaaa ccggacaagg ggtgacggca tattgatcct tggcgcttcg
     2041 ccaaagttat accccttctt ccctgaacag tgttcaaaaa actgctggta gtgatgggca
     2101 tacggcccat acgctcgatc cacgctacca ttcatgttcc atccctcctc tccttcataa
     2161 agggcgcgca ggacactccc cctcggcttg ccgaagagct tcatctgaga aaaatcattt
     2221 catgtaaata tagttacagg catcattcgg aaaactctct ctcctgtcag aaccaactcc
     2281 aaccacctgc catcccccat cggttgaacc cgcgcctgaa aaaatgtctt gaccgggcga
     2341 ggtactggag gtgttgacgc acctccatac gtaatcaccc gttgcagttg gaacttgaaa
     2401 ccgcctagca aaatttcaat taatttcgaa agtgtcaaag tcaatgtggt caaagacacg
     2461 ctatggtggc cactttcact tgatatgttc tcagtaatct atccaattca gagagctact
     2521 gggtgggtct ccgaacgggc tgctaattta cttccagtgg gagcccgtat atttcacaaa
     2581 agtttccaag aacagccatc catgaggcgg acctcttgac cccaaaaact tttggtctta
     2641 tttgatagac ataggcgaac gtttctcagg ttcaacatcg cggtgtcgcg ggtaacattc
     2701 actggcaaat ttcatcagag tatgacggga ggtgtcagat gtttgagttg cttattatcg
     2761 gttgcattag cggggtcatc ttgatagatg cgctgattct gaaatggttc attggttttt
     2821 ttgaatgaac catccaagaa acagtcccaa actgttgaaa attccagagc tttaaagctt
     2881 gggtgttctt gatgtgacac aacccgctca aataagagtt cttttcacat actcgaatct
     2941 acttttggag gtccaacgat gagtcaatcc caactgagtc aaagatggaa tggaaggcgc
     3001 gtggccacct tcaccttcat tggtataggc ctgctaatcg ctggtatatg tttgggcctg
     3061 ggaatcgctg ctgactcgag tctcctgcca ggcctctcac cacaattgaa agttagtggt
     3121 acgcctcttt cggattcctc atcacaaccg tctggtgcac tctcatttgg gcaacaagac
     3181 gcccaaggtc agagcaccaa ccttattcag gtggctaaat tggcacgtcc cgctgtagtg
     3241 aatatttcca caaccgccac agccaaaacc ccggaaccct tgcgatcacc attttttgat
     3301 gatccattct ttcgtcgttt ttttggggat gagtttgagc ggcggttcga acctccccct
     3361 tcccctcgac aagaaggagg aggttcggga gtcattgtga gtgacgatgg gtatattatt
     3421 acgaataatc atgtgattga aaatagtgat gccattcaag tgctgttatc cgataagcga
     3481 acctttacag ccaaggtcat agggactgat cccaaaacgg acctggctct actcaaaata
     3541 gatgccaagg atctgcctgt tttgtcatgg ggggattccc agcaattaca ggtcggagaa
     3601 attgtgatgg ccgttggcaa tccatttggg ctgagccaaa ccgtcaccat ggggatcgtc
     3661 agtgcggtag gtcgggccaa tgtggggatt gtcgattatg aagacttcat tcagaccgat
     3721 gcggcgatta atcccggcaa ctcgggtggg gccttagtga atcttcaggg agagttgatc
     3781 gggatcaata cggccatttt tagccggtcc gggggatata tgggcattgg gtttgccatt
     3841 cccagtaaca tggccaagtc tattcaggcc agtcttattc agcatggaaa agtggttcga
     3901 ggatggctgg gtgtttcgat tcaggatctc acaccagacc ttcaaaccca atttgatact
     3961 cctgatacgc agggggccct ggtcagcgaa gtgatggaag acagccctgc gaaaaaggca
     4021 ggtatccaac gcggagatat cattcgaaaa tatgattcgc agaccgtcgc cgacactcgg
     4081 catctccgat cgttggtggc agaaagtgtg cccaattccc ccgtaacatt acatgtcctc
     4141 cgggatggaa acccccgtga agtacaagtg accctttcgg aaatgcctaa agatgtcaga
     4201 gcgctcgcct cgacaggaga attgagagga catcatgcct tatcggggat caccgcggaa
     4261 tccatgccct taggggaaaa gggcgtgaaa gtgacaaagg ttgatcctga gagtactgct
     4321 ttccgggccg gcatacggga aggcgacatt attctcgaaa ttaaccgtga agccgtcaac
     4381 aacgcggatg actttcaacg gttaacaggg aaactgggat ccaaggatag agttttgctg
     4441 ctcctccagc gcggacggag tacgatcttt ttctcggtta ccccttaatt ttatgggggc
     4501 aggtttctac atcatcctgg aaaatggggg cactccttta gtgcccccaa aaccgaaaaa
     4561 ctgagccacc acaaatttag tcatgaaatt tattgagcgt gcgtatcctg cgtcgtgcga
     4621 cacgaactat catctgagtc ccccggccca tagctacccc gctacgaaat tttctcaaaa
     4681 aggcgtttac ggcctcatcc tgggctcttg tattctttta gggaaacacg ctagttttac
     4741 accttgttgt tctccatgaa ccgagccctt tcttaaatta gtcaaggatt tctatctatg
     4801 gcatccgcac aacgcgatct ttacgacata ctgggggtca aaaaaaccgt tgacgccaaa
     4861 gagctcaaaa aagcctatcg tcgactggca cgccaatatc atccggattt acatcccggc
     4921 gaaaaaaaga cggaaatgga aaagaaattc aaagagctga atgaagccta tgaagtcctt
     4981 ggggatgagg aaaacagaaa aaagtacgat caatacggcc tgaactggaa ggaagccgag
     5041 gcctatgaac gggcccgcca acagggcggc ggagcgtatc cggggggagg tcctggtgga
     5101 tttactcagg gaggtgctga tttcagtgat atttttgaga gcatgttccg ccagggggct
     5161 caacgggaag gggcaggctt tcgcggcttc gcgatggcgg gggccgacct ggaagccaac
     5221 ctgccgatct cactccggga agccttcacc ggcacgcgcc gcgccttaaa tctttctgat
     5281 gccgggggaa cgcctcgctc aatcgaagtt cgcattccgg ccggagttcg agatggcgaa
     5341 cgtcttcggg tgaaaggaaa aggcgcgcct ggtcgcggag gaggaccgcc aggggattta
     5401 ttttttcatg tacaaattgc ccctcatccc gtgtttcaac gcaaagattc agatattgtg
     5461 gtcacgctcc cgctttggcc gtgggaggcc gtattgggca ccgacattcc ggtgcccacg
     5521 ttatcaggaa cagtccgtct gaaaattccc gccggcagcc aatcccaaca acgaatgcga
     5581 ttaaagggca agggactgcc aaaacgaaca ggagggcatg gagatcaatt tgtggttctg
     5641 gacatcgtca cgcctgagac tctttcttcc gaggaacaac aactctatga gcaattagct
     5701 aagttcgatc accctgatcc ccgttcatcc cttcttcggg aggccgccaa tgactgacga
     5761 ctcagaacca tccttagaac aggttaccgc ggaaatcatc acctttggga gaattcgtcg
     5821 tgaagaagtc tgtacgagat tagggatcgg cgaagacatg ctggaggtct gtttgcaatg
     5881 ggaaattatt ccaccaccgg aaccggaccc ggaggggatg gtgttgtttc ccgaagacgc
     5941 cattgaccgg ttaagccgcg ggctccggtt acatcgagac ttagggatta actggccggg
     6001 agtcagcgtg gctttggaat tgttggaccg tatcgaagaa ctggaacaac agatccacaa
     6061 tctttccagc cagtaagctc aagcggtccc tcggttctca agaacaactt tcgagaacga
     6121 cgcaacagac cctcccttcc taactgccga ccagagaacg atccggtctt cctccccgcc
     6181 cccttgtaga tgtcgaatag gaacacaaag ggattacccc gccaaccggt gtggccaatc
     6241 atctaagaaa ccaagccgag aagcgaatcc ggctcttcac ttttctctct gtcagttcta
     6301 tctgtacttt cccaaacctc tatccggttt gcctccactc aatcccgaac cttggccagg
     6361 catcttagcg agcacacacg agaccttaat tctccatctt ttgaggcatg ttcttgtccg
     6421 tctcaccccg gctataaatt gtagtcacta cggcgctcca tggaatcgga aatcatttgg
     6481 atttcacctg gcctattggc acccaagaaa ataatggaaa agcccaatga gatcatccaa
     6541 caatcaatct gtacggtacg gcaatcatca gaagaagaag cgcttcatga ggtttctctt
     6601 catcattttc atcggcatcc tgttgtcagc gccggtggca acagccgtcg gttccctatc
     6661 gggcgaactg attaatcgac ttttcttaac cgtgaatgag acatggatcc aataataata
     6721 ttgtgccttt ctccccaagc aagacacctc atatatcagc gccgctccca cgaagcccag
     6781 ataaaagctc tcaccccgca acttgacacc acccgtgaca ttctaaagtc tgcgatttaa
     6841 accgacggtg ggccatatcc cgccattggc aagctgtctg acgggacagc tccctttcca
     6901 agttcaacaa acatgagtag aaaaatcatt tacctccact atggtggatt ggggcaaagt
     6961 gggacggatg aacgctttcg ctatgtggca ggatgggaca ttttcaacct gccttctcat
     7021 tcaataaata gtcaacgaca tcattttttt tcttgtggct aacattttcg ttctttccta
     7081 tacagaaaga ttcgctcaca aacggcggca tgaatcttgg atttgttaaa gattggcgtc
     7141 acacggggaa taaattttta caaaaagcga ggcactaatg atggcaatat caaaacggct
     7201 aggttattcc acatcgtaca agaagaaaga actgacaggg gatgatccct attcaatgaa
     7261 agcggccccc aaggggttcc ttcaatgtcc ggaatgtcag gccgtgtatc accgaaaacg
     7321 ttggagtttt ccaggaacgt cctcgcctcg ttcctcaaaa tcaaccgcag gtacccccaa
     7381 aaaaccaacc aggcaagtta tggttccaca gagttttgtc tgtcctgcct gccggaaatt
     7441 gcgagacggc tatgcggaag gctacctcgc cattcactgg ccgaattggg gcacacacaa
     7501 agctgaggtc ttaggtctca ttcataacga ggaaagtcag gcgagtcgca acaatcctct
     7561 ggaacggatc atggccattc gcacacgtcc tgacggagcg gatatcgaaa caactacgga
     7621 acactttgca caacgcctgg gcaaacatct cgatcgggct ttcaaaggaa agatcaccta
     7681 ccaatggtca cataaggaca aattggcgag agtcgaatgg caaggcccca agcgcccggt
     7741 acttccgcgc caaaaatcca aaacatccca aactaaaaaa taaagcggac cgctttcgca
     7801 tccctggcct cacccgatta tgctctatgg acattcacaa ggagggacat ctcatgaaat
     7861 cccgcatcat ggtttggagt ctgatactgc tgatcgggtg ggccataccc actggagccc
     7921 aggaaccgca agttgatccc ttacaaggcg aggttgtata tcaagcccac tgcgtgaggt
     7981 gccacggtat ccagggaaaa ggcgatggac cggacgctgc cgcgctcgtc gtcccgcccg
     8041 ccaattttca acgaatggaa tcccgggcca aatcggagat ggacctccgt tcagcaatca
     8101 tctggggact ggccttcagt cctat
//
LOCUS       DATIAL010000149         4499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_26456, whole genome shotgun sequence.
ACCESSION   DATIAL010000149 DATIAL010000000
VERSION     DATIAL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26456"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /gene="nadA"
                     /locus_tag="VLA60_04755"
     CDS             <1..617
                     /gene="nadA"
                     /locus_tag="VLA60_04755"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HSF08699.1"
                     /translation="KAIMNWAWKRREKILFFPDEHLGRNTANAMGFPREEMIVWDPFQ
                     PNGGHTRETVQKARLILWKGHCSVHQMFQPAHVAYFRKQYPAVQVIVHPECHEDVVNQ
                     ADLVGSTEFIIRTVTQADPGTIWAVGTELNLVNRLKHDQTDKQIYFLSPMVCRCSTMY
                     RIDGPHLCWAMENLVQGHVVNHIQVPDEEKVFAKLALDRMMDLS"
     gene            793..3651
                     /gene="ileS"
                     /locus_tag="VLA60_04760"
     CDS             793..3651
                     /gene="ileS"
                     /locus_tag="VLA60_04760"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HSF08700.1"
                     /translation="MDYKATLNLPRTEFPMKANLPQKEPERLAWWEQVRVYDRIQEAR
                     NGSSRYILHDGPPYANGHIHIGHGLNKILKDIIVKSKTMAGFQAPYIPGWDCHGLPIE
                     HQVTKQLGPKKKDLSAVELRKLCREYAEKFVEIQRDEFRRLGVFGDWEHPYLTMDHAY
                     EAAIVREFGKFVEKGRVYKGLKPVLWCPVDQTALAEAEVEYEDHLSPSVYVKFPFTVS
                     PTEMGSDQRLDLPTVKMLKAVSAVIWTTTPWTLPANQAICLHPDFDYAVLQAGEEAFI
                     VALGLEDAFAKACNIEKFEVLGKKKGKDFEGWTCHPPFAKKEVPILNGNFVTLEQGTG
                     CVHIAPGHGMDDYLLVLKYNKSPYVEILPDRKPLEILVPVNDAGRFTDEFPDFAGQPV
                     LEANPGIVEVLRTKGLLVGEKKLEHSYPHCWRCKQPVIFRATHQWFVSMEVGDLRRKA
                     LKEIDKVQWIPERGRDRIYGMIVNRPDWCLSRQRMWGTPIPAFSCESCSTPLADHNVI
                     EHISVQMQESGADIWFSRSAEELLPHGITCVQCGHGSFKKEHDILDVWFESGVSHAAV
                     LRPRGEGWYPADLYLEGSDQHRGWFHSSLLTSVTTDEQAPYRAVLTHGFVLDGEGKKM
                     SKSAGNVVTPQEIIKESGAEILRLWVASQDYQEDLRISKEILKQLTDAYRKVRNTCRF
                     LLSNLYDFEPGVHSVPHADLPELDQWALWRLGQLIDNVKKGYAQFQFRQVVHEWDYFC
                     STDMSATYLDILKDRLYTFPANSPLRRGSQTVLYEIVSALAKLMAPILSFTAEEIWQV
                     LPESTKEGSKRVSVHLADFPESPTVFQDPQLHTTWTYLFQVRGKVQFALEKSRRDKVI
                     GSSLDATVILHATEPNSSLLNRYLIDLPALFIVSCVKVEKVVTLPGADLVEASLGLAI
                     EVVKAEGTKCDRCWNIRQDVGSQAQHPTLCGRCVEALG"
     gene            3648..4169
                     /gene="lspA"
                     /locus_tag="VLA60_04765"
     CDS             3648..4169
                     /gene="lspA"
                     /locus_tag="VLA60_04765"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HSF08701.1"
                     /translation="MSGGIHRFVLLGLVVASIISLDQVTKYYIASSMYLHESIPIIPG
                     YFSLTYIRNPGAAFGIMGTTSSGFRLIFFFLTSLFAMGLLITIFLRLEPHDWWGQMTI
                     ASIFGGAIGNFIDRLQYGEVIDFLDFYINGYHWPAFNVADSAISVGVCSLLLLFAFEK
                     RKPNLTPQENPPL"
     gene            4262..>4499
                     /locus_tag="VLA60_04770"
     CDS             4262..>4499
                     /locus_tag="VLA60_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08702.1"
                     /translation="MFPSTAYLRPNGVGLSIFWIVLLLSGSASFGEELQLPVNTCPSE
                     GKPIHIHVHGIKSAAGSLKAVLYGPDPESFLVKGA"
BASE COUNT         1040 a         1010 c         1228 g         1221 t
ORIGIN      
        1 ccaaagcgat tatgaattgg gcctggaaac ggagagaaaa gattctcttt ttccccgatg
       61 agcatttagg gcggaacacc gcgaatgcca tggggtttcc ccgcgaagag atgattgtgt
      121 gggatccctt ccaacccaac ggcggacata cccgggaaac agtacagaag gctcgtctga
      181 ttctctggaa gggacactgt agcgtgcacc agatgttcca gccggcacac gtggcctatt
      241 tccggaagca gtatcctgcc gttcaggtta ttgtgcaccc tgaatgtcat gaagatgtgg
      301 tcaatcaggc agacctggtg ggatcgacgg aatttattat tcggacggtg acccaggccg
      361 atccgggaac aatttgggca gtgggaactg agctgaattt ggtgaatcgg ttgaaacatg
      421 atcaaaccga taagcaaatc tatttccttt ccccaatggt atgtcggtgt tcgaccatgt
      481 acagaattga tggacctcat ttatgttggg ccatggaaaa tctcgtgcag ggccatgtgg
      541 ttaatcacat acaagttccc gacgaggaga aagtgtttgc caagctggct ttggaccgca
      601 tgatggatct gagctgattg ccttcctttt gtggtttctt cttcccttaa tttgtggccc
      661 tttcatatca agaatagggt tctctccctt tgatgtgttg tggcgatgtg actgaatggc
      721 gttgtttgac gggtttgagg ggcatggcta gaatggcctt gagtttatcc cattattctt
      781 gagaatttcc ccatggacta caaagcgaca ttgaaccttc cccggactga gtttccgatg
      841 aaggccaatc tgccacaaaa ggaaccggag cgattagcct ggtgggagca ggtgcgggtg
      901 tacgaccgta ttcaggaggc cagaaatggc agctcccgtt atatccttca tgatggccct
      961 ccctatgcca atgggcatat tcacatcggg catggcctga ataaaatcct caaagacatc
     1021 attgttaaat cgaaaaccat ggcgggtttt caggctccct atatccctgg gtgggattgt
     1081 cacgggttgc cgattgagca tcaggtgacc aagcaacttg gacccaagaa aaaagatctg
     1141 agtgccgtgg aactccggaa actctgccga gaatatgcgg aaaaatttgt tgagatccaa
     1201 cgagacgaat ttcgccggtt aggagtgttc ggggattggg agcatccgta tctgaccatg
     1261 gatcatgcct atgaggcagc gattgtccgg gagttcggga agtttgttga aaaggggcgg
     1321 gtctataagg ggttgaagcc ggtcttgtgg tgtcccgtgg atcaaacggc gctggctgaa
     1381 gccgaagtgg aatacgagga tcatctctca ccgtcggtgt atgtgaaatt tccctttacc
     1441 gtctcgccaa ctgaaatggg ttccgaccaa cgattggatc tgccaacagt caaaatgttg
     1501 aaagccgttt cggctgttat ctggacgacg accccttgga cccttcccgc gaatcaagcc
     1561 atttgcctgc atcccgattt tgactatgcg gttcttcaag ccggtgaaga ggcttttatc
     1621 gtggctcttg gattggaaga cgcatttgcc aaggcctgta acattgagaa atttgaggta
     1681 ttgggtaaaa agaaaggaaa agattttgag gggtggacgt gtcatccacc atttgccaag
     1741 aaagaggtcc ccatcctcaa tggaaatttt gtcacattgg agcaagggac ggggtgtgtc
     1801 cacattgctc ccggacatgg gatggatgat tatcttttag ttctgaaata taacaaatcc
     1861 ccttatgtcg aaatacttcc tgatcgaaag cctttagaga tattggtgcc ggtcaatgat
     1921 gctggacggt ttacggatga attccctgat tttgccgggc aacccgtttt ggaagcgaat
     1981 cccgggattg tagaagtctt aaggaccaaa ggtttgttgg ttggcgaaaa aaagctcgag
     2041 cattcttatc cacattgttg gcgctgtaag caaccggtca tctttcgggc tactcaccaa
     2101 tggtttgtat ccatggaggt aggcgatctt cgtcggaaag ccttgaaaga aattgataaa
     2161 gttcaatgga ttcctgagcg ggggcgagat cgtatttatg gcatgattgt gaaccgtccg
     2221 gattggtgcc tatcccggca acgcatgtgg gggaccccca ttcccgcgtt ttcgtgcgaa
     2281 agttgttcga ccccgcttgc tgatcataat gtgattgagc atatcagtgt tcaaatgcag
     2341 gagagtggag ccgacatttg gtttagtcga tccgccgagg aattacttcc gcatggaatc
     2401 acatgtgtgc aatgcggcca tgggtcgttt aaaaaagaac atgatatttt agatgtctgg
     2461 tttgaatcgg gcgtgagtca tgcggccgtg ttgaggcctc ggggcgaagg ctggtatccg
     2521 gcggacctct atcttgaagg gtccgatcaa catcgggggt ggttccacag ttcgctcttg
     2581 acgtccgtga cgacggatga acaagcgccc tatcgggccg tgctcacgca tgggtttgtt
     2641 ttggatgggg aaggcaagaa aatgtcgaaa tcggcaggca atgtcgtgac cccacaagaa
     2701 atcatcaaag agtctggtgc ggaaattctg cgactatggg tggcctcgca ggattatcag
     2761 gaagatctgc gtatctccaa agagatttta aaacaattga cggatgcgta tcgtaaggtg
     2821 cgtaatacct gccggttcct tctgagtaac ctgtatgatt ttgagccagg ggttcattcc
     2881 gtgccccatg ccgatttgcc ggaattggat caatgggcac tctggcggct cgggcaactg
     2941 atcgacaatg tgaaaaaggg atatgcccaa tttcaatttc gacaggttgt gcatgagtgg
     3001 gattattttt gttccacgga tatgagtgcg acctacctgg atatcttgaa ggaccggttg
     3061 tatacctttc cggcgaactc gccgcttcgg cgtgggtctc aaacggtctt gtatgaaatt
     3121 gtgtcagcct tagccaagct catggcgcct atcctgagct ttacggctga agaaatctgg
     3181 caggttcttc cggaatccac gaaagaagga tctaaacggg ttagtgtgca tttggccgat
     3241 tttccggaat cccccaccgt ctttcaagac ccccagctcc acacgacgtg gacctacttg
     3301 ttccaagttc ggggtaaggt gcaattcgcc ttagagaagt cgagacggga caaggtgatt
     3361 ggttcttccc tggatgccac cgtgattttg catgcgactg aacccaattc ttcgttgctc
     3421 aatcgctacc tcatcgattt gcctgcactg tttattgtct cgtgcgtgaa ggtggaaaag
     3481 gttgtgaccc tgcctggagc cgacttggta gaggcgagtc tcggtttggc gattgaggtc
     3541 gtcaaagccg aaggcaccaa atgtgatcga tgttggaata tccgccagga tgtgggctca
     3601 caggctcaac atccaaccct ctgcgggcga tgcgtggagg cgttggggtg agcgggggca
     3661 ttcatcggtt tgtgttgtta ggcctcgtcg tcgcgtccat tataagcctt gaccaggtca
     3721 ccaagtatta tattgccagt tcgatgtatc ttcatgaatc catccccatt attccgggat
     3781 atttttcttt gacctacatc aggaatcctg gcgccgcatt tgggatcatg ggcacaacca
     3841 gcagcgggtt taggctcatt ttctttttcc tgacttccct ctttgccatg ggattattaa
     3901 tcaccatctt cttacggctg gaacctcacg attggtgggg gcaaatgacc attgcgtcta
     3961 tttttggggg tgcgattgga aattttatcg atcgcctgca atatggagag gtcatcgatt
     4021 ttttagattt ctatatcaat ggctaccatt ggcccgcttt caatgtggcc gattcggcga
     4081 ttagcgtggg ggtgtgctct cttctcctgc tgtttgcctt tgaaaaacgg aaacccaact
     4141 tgacccctca agagaatcct cctctctaat cttccggaat atctgcagtt gcgagtcacg
     4201 tgtgtacccg cttattttgc tctcatcata tgatgttcat gtctaagtat ggagggtgaa
     4261 gatgtttccg agcactgcct atttgcgacc aaatggggtt ggcctgtcta ttttttggat
     4321 agtgttgttg ctcagtggtt cggcaagttt cggtgaagag ttgcagctac cggttaatac
     4381 ctgcccctct gaagggaagc ccattcacat tcatgtgcat ggaatcaaaa gcgctgcagg
     4441 atcccttaag gccgtcctgt atggacctga tccggagagt tttttagtca agggagcca
//
LOCUS       DATIAL010000150         3628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1101476, whole genome shotgun sequence.
ACCESSION   DATIAL010000150 DATIAL010000000
VERSION     DATIAL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101476"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VLA60_04775"
     CDS             complement(<1..384)
                     /locus_tag="VLA60_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase"
                     /protein_id="HSF08703.1"
                     /translation="MSILATKDTHVVIQGGVAGVNAARRMAEFRYMIKQPLNVSAFVY
                     PPDAGKTNEIICGTELIMIPIYKTVAEATANHPEINTSLVYIGADRAYAGTMEALNDP
                     HIKTVSMITEGVPEKDAKLLGKHARK"
     gene            complement(420..1616)
                     /locus_tag="VLA60_04780"
     CDS             complement(420..1616)
                     /locus_tag="VLA60_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HSF08704.1"
                     /translation="MAKVLEGPGMGLLRKWGISTPNYAVVTSSEEFDQLSQVNDWLQK
                     NKLVVKAHEALGSRFKLGLVKVGLDLAAAKVAVREMLGKKLETLTIKQVIVSEAVDHK
                     EEYYAAAKSTRDGVDLLVATCGGIEVESNWDKVKQVSIETGETLSDSALSSLVKDAGF
                     SGDTAAKMAEFLKKLYACFDNEDAQYVEVNPVVLSGQGELVALDAVTLLDGDAKFRHK
                     DWTFPFAAEFGRAYTKNEEEVMAVDAKVKGSVKLIEIPGGNIAMLPAGGGASVYYSDA
                     VVARGGKLANYAEYSGDPPDWAVEVLTEKVCSLPGIKHIIVGGAIANFTDVKKTFGGI
                     INAFRKAKAEGKLEGVKIWVRRGGPNELQGLAAMRALQDEGFDIQVFDRKTPLTDIVD
                     MAMAAK"
     gene            complement(2067..2744)
                     /locus_tag="VLA60_04785"
     CDS             complement(2067..2744)
                     /locus_tag="VLA60_04785"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF08705.1"
                     /translation="MSHVKVVTLPKIGDVPIVCKTKRDISCETPLDARDDESLGWGYW
                     DRIWPSEVALSECLIHQFFPTKLQGVKVLEIGCGTGLAGVVAARLGAFTMFSDMVPIT
                     LEATKESCRLNHISNFDTCLLNWTEKIGTKKFFYDLVIGSEVFYDEGILAGISHILGQ
                     VLCPKGKAMFCDPNRLGLDTVEHGFKEKFIVAIEEVPLNWPPRKDAGVEKKGFLYQLS
                     RRSQEAF"
     gene            complement(2855..>3628)
                     /locus_tag="VLA60_04790"
     CDS             complement(2855..>3628)
                     /locus_tag="VLA60_04790"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSF08706.1"
                     /translation="VFWLNSNRAHDAQVIEKVNLYLKDHDTKGLDIQIMAPVDAMKFS
                     LKRAKDGLDTISATGNVLRDYLTDLFPILELGTSAKMLSIVPLINGGGLFETGAGGSA
                     PKHVQQFVKEGHLRWDSLGEFLALAESFAHLSQVKNNKKAKILADTLDKATGKLMDNR
                     KAPGRELGQLDNAGSHVYEAMYWAQELAAQNDDQDLKKQFAPIAKELESKVDTILEEI
                     KAAKGRAQDIGGYYRPDPTKVMAAMRPSATFNAILSRLG"
BASE COUNT          913 a         1031 c          742 g          942 t
ORIGIN      
        1 cttacgggca tgcttcccca aaagtttggc atccttttcc ggcacccctt cggtaatcat
       61 ggagaccgtt ttaatatgcg ggtcattgag ggcttccatg gtgccggcat aggcccggtc
      121 tgctccgata tagaccagac tcgtgttaat ctccgggtgg ttggccgtcg cttcggccac
      181 ggttttataa atgggaatca ttattaactc agttccacaa atgatctcgt tagtcttccc
      241 cgcatcaggg ggataaacga atgcagagac atttaatggt tgcttaatca tgtaccggaa
      301 ctcagccata cgacgagcgg cattcacccc ggccaccccc ccttgaatga cgacatgcgt
      361 gtctttcgtt gcaagaatac tcatcgaaac ggtctcctac atcctcaagg tctaataggt
      421 tattttgcgg ccatggccat atcgacaatg tccgttaatg gcgttttgcg gtcaaatacc
      481 tgaatatcaa aaccctcatc ctggagcgca cgcatggccg ctaatccctg caattcgttt
      541 ggaccacccc ggcgcaccca aatcttgaca ccctctagct tgccttccgc tttggccttt
      601 cgaaacgcgt tgataatgcc cccaaaggtc tttttgacat cggtaaaatt cgcaatcgct
      661 ccacccacaa taatatgctt gataccaggc aacgagcaga ctttttccgt cagaacttcc
      721 actgcccagt ccggtggatc gcctgaatat tctgcataat tcgcaagttt gccaccacgt
      781 gccaccaccg catctgaata ataaacgctg gcaccaccac cggcaggtaa catcgcaata
      841 ttgccgccgg gaatctcaat gagcttgacg gagcctttca ccttggcatc aaccgccatg
      901 acttcctctt catttttggt ataggcacgc ccaaattcag ccgcaaacgg aaaggtccag
      961 tccttatgcc ggaacttggc atcaccgtcc agaagggtca ccgcgtccag agccacaagt
     1021 tctccctggc cactgagtac aactggattc acttctacgt attgagcatc ttcattatca
     1081 aaacaggcat ataatttctt caaaaattcc gccatttttg cagccgtatc gccagaaaac
     1141 cccgcatcct ttaccaggga ggaaagcgcg gaatccgaca gggtctcacc ggtttcgatg
     1201 gatacctgct tgaccttatc ccaattagat tccacttcga tgccaccaca ggttgccacc
     1261 aagaggtcca ctccatcacg cgtcgatttg gccgcggcat aatattcttc tttgtgatca
     1321 accgcctcgg aaacaatcac ctgcttaatc gtcagggttt ccagtttttt accaagcatc
     1381 tcccgcacag ccacttttgc cgcggctaaa tccaagccca ctttgactaa ccccaattta
     1441 aaacgagagc caagtgcttc atgcgctttg actaccagtt tatttttttg gagccaatca
     1501 ttgacctgag ataattggtc aaattcctca ctcgaggtca cgacagcata gttgggagtt
     1561 gaaatccccc attttcgcaa taagcccatt cccgggcctt caagaacttt tgccataatc
     1621 caacccttcc ttcagggact catcataatg gttaaggaca aacgtagcac aacccgcctg
     1681 ccgcgcggaa cggtgcattg tacgaatgcc ctcatcccgc tgcaatcaat caaaagaccc
     1741 attaagccat ttcaaaagtc ccagtgtcac cacgtggaat gtccataaga acaccgccac
     1801 accctaaaga aaaaacctat ttaaaattag ttagttgaga tgagaagtcc ccatccctaa
     1861 ccttagctcc aggctttcct taaacaaccc cgcactagcc tgcgctctct cctgctatga
     1921 gacaaaaccc tgccctgccc tagccattca ttttgacgct ttaaaaccat taactttact
     1981 cactaaatct ttgaacttcg ggcaaccctg tgcttaagag cctcgtcctc attcatagcc
     2041 ccttcagcac gtggaaaatg atcttgtcag aatgcctcct gactcctgcg acttaattgg
     2101 taaagaaacc ccttcttttc tactccggca tcttttcggg gaggccagtt gagcgggact
     2161 tcttctattg ccacaataaa tttctcttta aatccatgtt cgacggtatc gagccccagt
     2221 ctatttggat cacaaaacat ggctttcccc tttgggcaaa gcacctgtcc aagaatatga
     2281 gaaataccgg ctaaaatgcc ttcgtcataa aaaacttcac tgcctatgac caaatcataa
     2341 aaaaactttt ttgttccaat cttttccgtc caattcaaca ggcatgtgtc aaaattgctg
     2401 atatgattca gacgacagga ctcctttgtt gcttcaagcg taataggcac catatctgaa
     2461 aacatggtaa atgccccgag ccgagcagca accactccgg ccaatcctgt tccgcagccg
     2521 atttccagaa ccttgacacc ctgcagtttt gttgggaaaa actgatgaat gagacattcg
     2581 gataaggcca cctcagaagg ccaaattcga tcccagtacc cccatccgag ggattcgtca
     2641 tcccgtgcat ctaatggggt ttcacaagag atgtctcgct tggtcttaca gacaatggga
     2701 acatccccga ttttgggaag ggtcaccact ttcacatgac tcacacgaat tatctccccc
     2761 caaaaaaggc gagccgggtt ccgattggaa cccggctcgt tgcacacatc cccagtataa
     2821 caataatacc ggaagaccag cctacgttga gaagctagcc taagcgactt aagatggcat
     2881 tgaaagtcgc gctgggacgc atggcggcca tcaccttggt tggatccgga cgataatatc
     2941 cgccaatatc ctgagcccga ccctttgccg ctttaatctc ttcaagaatt gtatcgactt
     3001 tggattccaa ctcttttgcg atcggggcaa attgcttttt caagtcctga tcatcattct
     3061 gtgcggcgag ttcttgcgcc cagtacatgg cctcgtacac atgacttccc gcattatcca
     3121 attgccccaa ttcccgtcct ggggccttcc ggttgtccat gagctttccg gtggccttgt
     3181 ccaaagtgtc cgccaggatt tttgcttttt tgttgttttt gacttgactc aaatgggcaa
     3241 acgactcggc taacgcgaga aactctccga gggaatccca gcgtaaatgt ccttctttga
     3301 caaactgctg tacatgcttc ggggccgacc ctccggctcc ggtttcaaac agtcctccgc
     3361 cattgatgag tggaacgatg gacagcattt tggcgctcgt ccccaactcc aaaatcggga
     3421 ataagtcagt gagataatcc cgaagcacat ttccagtggc cgaaatggta tcgaggccgt
     3481 cctttgcacg tttgagtgaa aacttcatgg catccaccgg tgccataatt tggatgtcca
     3541 gccctttagt atcgtgatcc ttaaggtaga gattgacttt ttcaatgact tgggcgtcat
     3601 gagcccgatt gctattgagc cagaatac
//
LOCUS       DATIAL010000151         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_134303, whole genome shotgun sequence.
ACCESSION   DATIAL010000151 DATIAL010000000
VERSION     DATIAL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134303"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VLA60_04795"
     CDS             <1..655
                     /locus_tag="VLA60_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08707.1"
                     /translation="LALKPGEYVRVSVTDQGMGIPQDHLSKIFDPYFTTKQKGSGLGL
                     ATTYSIIKRHEGHIAVSSSLGKGTCFTFYLPASATISASNNETPEALLQGTGRILVMD
                     DEEEIRDVLGKMLQHLGFEVDLVNDGQQALTLYGRAIQGGTPYVATILDLTIPGGMGG
                     KETLRRIKEHHPHAKVIVSSGYSNDPVLAHATQFGFSGFIAKPYNLADLSRVLHRIL"
     gene            complement(673..1713)
                     /gene="mtnA"
                     /locus_tag="VLA60_04800"
     CDS             complement(673..1713)
                     /gene="mtnA"
                     /locus_tag="VLA60_04800"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSF08708.1"
                     /translation="MVPTVEWKQDRVVILDQSALPGEVRFLKCETYQDVAEAIRNLKV
                     RGAPAIGVTAALGIALGARQYAETHIEGFLQFMEQVCHTLASTRPTAVNLFWAVDRMK
                     RVLAKASLTSVQDMQKQLQEESLAILEEDIRVNRNIGKFGSAVIRDQDHILTHCNAGA
                     LATAGYGTALGVIRAAWGQGKKIRVYADETRPVLQGARLTAWELQQDGIPVTLITDNA
                     AGALMKQHMIQCCVVGADRIAANGDVANKIGTYSVAVLANAHDIPFYVAAPSSTIDMQ
                     TPDGDGIPIEERSPSEVTSLYGERSIAPENIEVWNPAFDVTPAGLITGIITERGIFSP
                     NDLKKNFPGNLS"
     gene            complement(1936..>3154)
                     /locus_tag="VLA60_04805"
     CDS             complement(1936..>3154)
                     /locus_tag="VLA60_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HSF08709.1"
                     /translation="RWNTKGWVALTDGVVALRGVQNPVSNLFVRLKIDRDQLDLKRME
                     FRVKDSEAVITGFMKDWKTTPTVSVMFESPQFDLELLIPKEKRSAIRDGIEWLASHGT
                     LEGSVHIERPTYKNFSGKKLSAALKIHDNLVSVEKVQTMVENHGTLGGRFFVHLPQGK
                     PAAMRVSFQANDVPFEKILTMIGDERRLITGNMNIRGMIQGHGRDRNGVVPTLNGSVD
                     FSLREGYVRKGTILPRILALLNLPNVLRGKVDLEETGFPFKKVQSKVKIEEGNFVTKN
                     FLLNSSIMKISAAGMYDLEHDRLEGLAAVSPFGAYSDLLKKIPLFGRIFSGDRKGIAT
                     AMFSIAGPLDDPKVDYMPGESLKTGLTGLAQLAIDILTNTITLPYELLKEANDNGSSA
                     PPESERSGSAKQP"
BASE COUNT          781 a          887 c          682 g          804 t
ORIGIN      
        1 ccttgctctg aagcccgggg aatatgtacg cgtctcagtc acggaccagg gtatgggcat
       61 tccccaggat catttatcaa aaatcttcga cccctatttt acgaccaaac aaaaaggcag
      121 cggattagga ttagccacga cctattccat cattaaacgg catgaaggtc atattgccgt
      181 atcatcctct cttggaaaag gcacatgttt tacgttctat cttccggctt cagccaccat
      241 atcagcgtca aacaacgaaa ccccggaagc cctccttcag ggtaccgggc ggattctggt
      301 gatggatgat gaggaagaaa tccgggacgt gttaggaaaa atgcttcaac atcttggatt
      361 cgaggttgat cttgtaaatg acgggcaaca ggcgctcacc ctctacgggc gtgccattca
      421 gggcggcacc ccatacgtcg caaccattct cgatctcacg ataccggggg gaatgggagg
      481 caaagaaacc ttacgcagaa tcaaagaaca tcatccacat gccaaagtaa ttgtttcaag
      541 cgggtattcc aacgatccgg tcctggctca tgccacccaa ttcggctttt ccggttttat
      601 tgccaaaccc tataatcttg ccgatctttc cagggtctta catcggatcc tctaaacgac
      661 gcacgcaaaa tcttaagata aattgcccgg aaagttcttt ttcaaatcat tgggagagaa
      721 aatcccccgt tcggtgataa ttcctgtgat caatccagcc ggggtcacgt caaatgccgg
      781 attccatacc tcaatattct caggagcaat gctccgctca ccatacaaac tggttacttc
      841 agacggactg cgttcttcaa tggggatgcc atctccatcc ggagtctgca tatcaatcgt
      901 agacgagggg gccgccacat aaaaaggaat atcatgagcg ttcgctaaca ccgccacgga
      961 ataggttccg atcttattgg ccacgtcacc attggccgca atcctgtccg ctcccacaac
     1021 gcaacattgg atcatatgtt gtttcatcag cgctcccgcc gcattatccg taattaaagt
     1081 caccggaatc ccatcctgct gaagctccca tgcggtgagc cgtgcaccct ggagcaccgg
     1141 ccgcgtttca tccgcatata cccgaatctt tttgccctgt ccccacgccg ctctgatcac
     1201 tcccaacgcg gtcccatatc cagccgtcgc cagagccccg gcgttacaat gcgtgagaat
     1261 atggtcttga tcccgaatga ccgctgaacc gaattttccg atgttcctgt tcacccggat
     1321 atcttcctca agaatagcca gcgattcttc ctgtaattgc ttttgcatgt cttgtacgga
     1381 agtcagggag gccttcgcca gcactcgttt catccgatca accgcccaga ataaattcac
     1441 tgccgtggga cgggtggagg cgagggtgtg acacacctgc tccataaact gaagaaaccc
     1501 ttcaatgtga gtctccgcat attgcctggc ccctaaggca atgcccaatg ccgcggtgac
     1561 cccgatggcc ggagcccctc gcaccttcaa atttctaatg gcttcagcca catcctgata
     1621 tgtctcacat ttgagaaacc gtacctcgcc aggaagtgca ctttgatcca aaatcacgac
     1681 acgatcctgt ttccattcaa cggtgggaac catgttgaca tcctttcgac agttatagct
     1741 cactcccttg tatcggtact gtgaatagat tgccttcccc ttcctccatg tcaggactgc
     1801 atatcgcatc cctcacggag aaattttctc tcctgcgcat tcgccctggg cacgagccgg
     1861 catccgtcat gtgccgttgc aagactatac agaaatcctt gcaccccttt ttcagggagg
     1921 ttcaaggaaa ttccctcacg gctgcttggc agatccggat cgttccgatt caggaggagc
     1981 cgaagatcca ttgtcgtttg cctccttcaa caactcataa ggaagcgtaa tagtgtttgt
     2041 taaaatatca atagccaatt gggccaaccc tgtcaatccg gttttcaacg attctccggg
     2101 catataatcg accttgggat cgtctagtgg tcccgcaatg gaaaacatcg ccgtcgcaat
     2161 tcctttccgg tcccctgaaa agattcgccc aaagagcgga atttttttta acagatccga
     2221 atatgcgcca aacgggctga cggcggccaa accttccagc cggtcatgtt ccaggtcata
     2281 cattcctgct gcggagattt tcatgattga actgttgaga aggaaatttt ttgtcacaaa
     2341 attcccttct tcaattttga ccttgctttg cactttttta aacgggaaac ccgtctcttc
     2401 cagatccact tttccacgca ggacattggg gagattgagg agagccaaaa ttcgtggaag
     2461 gatcgttcct tttcggacat atccttctcg aagagaaaaa tccacacttc cattcaatgt
     2521 agggacgacc ccgttccgat cccgcccatg accttgaatc ataccacgaa tattcatgtt
     2581 cccggttatc aatcgccgct catcccctat catcgtcaag atcttttcaa acggcacgtc
     2641 gttggcttga aacgaaaccc gcatggctgc cggttttcct tgtggaagat gaacaaaaaa
     2701 tcgtcctccc aatgtgccat gattttcgac catggtttgc actttttcca ccgataccag
     2761 attgtcatga attttcaacg cggcagataa ttttttcccg gaaaaatttt tataggtcgg
     2821 ccgttcaata tgcacggagc cctcaagggt tccatgagag gctaaccact caatgccatc
     2881 ccggatggcc gatcgtttct ccttgggaat cagcaactcg agatcgaatt gaggcgattc
     2941 gaacatgaca ctgacggtgg gagtcgtctt ccaatctttc ataaatccgg tgatcacggc
     3001 ttcactatcc ttcacacgaa actccattcg tttcagatcc aattgatccc ggtctatttt
     3061 gagtcgcaca aagagattgg aaacgggatt ctgcacaccg cgaagggcga caactccatc
     3121 ggtcaaagcg acccatcctt tggtattcca cctg
//
LOCUS       DATIAL010000152         2176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1101711, whole genome shotgun sequence.
ACCESSION   DATIAL010000152 DATIAL010000000
VERSION     DATIAL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101711"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1819
                     /locus_tag="VLA60_04810"
     CDS             293..1819
                     /locus_tag="VLA60_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSF08710.1"
                     /translation="MLAKIQSVGLVGLEANLIEIEVDIGGGLPQFSIVGLPDATVRES
                     RDRVRSALKNTGFHFPAKKITVNLAPAGLKKEGAGLELGIAIGILVAEGVLSQETVTS
                     YIFVGELALDGRIKAVPGALSMAIATHHPFSLILPHDNASQAAVVDHATVFGVSTLPQ
                     VVEFLQGALTLLPAKNTFSIVQPGTSSIEEDFADVVGQYQAKRALEVAAAGGHNLLMV
                     GPPGSGKTMLAQRLTGILPPMSFQECLEASQVHSVAGNLPPHAALLANRPFRAPHHTI
                     SEAGLVGGGSIPRPGEVSLAHHGVLFLDEALEFKRSLLDSLRQPLENGTVTLTRAQAS
                     FTFPAKLMLIVAMNPCPCGYSGDPTHECICTPYHIQRYRSRLSGPLLDRLDIHIEVPA
                     VPVKELSGRITGEASSAIRQRVIQARHLQIDRYRAEQTLNNAQLKPRLLKKYCQLDQA
                     GNTLLDQAVTRLGLSARAYGRILRVARTIADLGESVMISSTHVAEAIQYRTLDRPILA
                     "
     gene            1829..2044
                     /locus_tag="VLA60_04815"
     CDS             1829..2044
                     /locus_tag="VLA60_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08711.1"
                     /translation="MESFKVFRWWFMIGALMALAVIMIQGGIRDLMLANEPIWEIKLV
                     ELGPPIFGGGLLGGCLALILNRIKDKS"
BASE COUNT          502 a          638 c          525 g          511 t
ORIGIN      
        1 cttccatccc ctccgaatcc accgtctctt acccctgcac cgaaactccc cgctctttca
       61 tcccccgtat ctcccccata agcactcgct cacagtgcaa attctcagaa gccccatgca
      121 tgagtccatc acacaaggcc gatatctctg aacccttatg aacggcagga accctcagca
      181 gcccgcaggc cgaatccgcc aaatgatttg gttgaagttt caaaaaaaag tgaccttgga
      241 tccgtgtatg cacagacacc acacgcctta aacgggaata gataagacaa gcatgttagc
      301 caaaattcaa agtgtgggat tggtgggatt agaagccaat ctcattgaaa ttgaggtgga
      361 tattggtgga ggccttcccc aattttccat cgtggggcta ccggatgcga ccgttcgtga
      421 aagccgggac cgggttcgat ccgcactcaa aaatacagga ttccattttc ctgcaaaaaa
      481 aatcactgtc aatttggcgc cagccggatt aaaaaaggag ggggcaggcc ttgagttggg
      541 aattgccatc ggcattttgg tagccgaagg ggtcctgtca caagaaaccg tcacctccta
      601 tatttttgtt ggggaactgg cactggatgg cagaatcaaa gcggttcccg gggcgctgtc
      661 tatggccatt gccacccatc accctttttc attgattctg cctcacgaca atgcctcgca
      721 ggctgccgtg gtcgaccacg ccaccgtgtt tggcgtctcg acccttccac aggtggtgga
      781 atttcttcaa ggggccctca ccctccttcc tgcaaaaaat acgttctcca tcgtgcaacc
      841 cggaacctcc tcgattgagg aagactttgc cgatgtggtg ggacaatatc aggccaaacg
      901 tgccctggag gtcgctgccg ccggtggtca caatcttctc atggtgggtc caccaggatc
      961 agggaaaacc atgttagccc aacggttaac aggtattctt ccacccatga gctttcagga
     1021 atgtttggaa gccagtcagg tccacagtgt ggccggaaac cttccacctc atgcggccct
     1081 cctcgccaat cgcccctttc gtgcccccca tcacaccatt tcagaagccg gcctcgttgg
     1141 aggaggatcc atcccccgtc ccggagaagt ctctctggct catcatggcg tcctcttttt
     1201 agacgaggca ttggaattta agcggtcgct tttggatagc ttacgccagc cactggaaaa
     1261 tggaaccgtc acgctgaccc gcgcacaggc cagtttcacg tttcccgcca aactcatgtt
     1321 gatcgtcgcg atgaatccct gcccatgtgg atattccgga gaccccaccc atgaatgcat
     1381 ctgtacgccc tatcacattc aacggtatcg aagccgtctg tccggcccac tccttgatcg
     1441 gctggatatc cacattgaag tccccgcagt cccggtcaaa gaactatcgg gcaggataac
     1501 cggtgaagcc tcttcagcca ttcgtcaacg ggtgatccag gcgcgacatc ttcaaatcga
     1561 ccgctaccgc gctgagcaga ccctaaacaa tgcacagctc aagcctcgct tgctcaaaaa
     1621 gtattgccaa ctggatcaag ccggaaacac cttgttggat caagccgtca cccgcctggg
     1681 attgtccgca cgggcctatg gccggatttt acgtgtggcc cgaactattg ccgacttggg
     1741 agaatcggtt atgatttcat ccacacatgt cgccgaagcg attcagtacc gaacattaga
     1801 tcgacccatt ctggcataag gacatttcat ggaatcgttt aaagtgtttc gatggtggtt
     1861 catgattggc gccctcatgg cactggctgt catcatgatt caaggcggga ttcgcgatct
     1921 gatgctggct aatgaaccta tatgggaaat taaactggtc gagttgggac cgcccatctt
     1981 tgggggaggc ctcttgggtg gatgccttgc cctcatcctg aatcgaatca aggataagtc
     2041 ctaacttcag ttacgtgtga cggccttccc gcattctctc ccacacatcc caagccctct
     2101 caccttctca aggagcgctc aaagcttgtc ctgatcctgg ccgaaggaag gcgcgttcag
     2161 ccatgtcctg gggctt
//
LOCUS       DATIAL010000153        16469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_779699, whole genome shotgun sequence.
ACCESSION   DATIAL010000153 DATIAL010000000
VERSION     DATIAL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16469
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779699"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VLA60_04820"
     CDS             complement(<1..318)
                     /locus_tag="VLA60_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08712.1"
                     /translation="MLSHLTPQGILSDRTNVHDRLHRRCAKWQKHILLVAFVTGLLPN
                     SAMGEAFVPKQAQSVFASPSYLHLVAQKPLGAEELNTIAVFEKAAQAVVYITNTAVRR
                     DIWS"
     gene            complement(338..760)
                     /locus_tag="VLA60_04825"
     CDS             complement(338..760)
                     /locus_tag="VLA60_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HSF08713.1"
                     /translation="MAVITHYLNISMTGNSTIENLTKPIHDLLAESGLQSGMLTLFVQ
                     HTTASVMIIEDEPGIRADTKTFWDRTIPADPQLEHNTRNAGEDNGHSHLRGQLQGPSL
                     TIPFVNGALTLGTWQQLVLIDFDTRPRTRNLVVQLMGE"
     gene            complement(769..1734)
                     /locus_tag="VLA60_04830"
     CDS             complement(769..1734)
                     /locus_tag="VLA60_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08714.1"
                     /translation="MTPPQAWDILPHGSTLHNPYAFRHEDCTILAVVSRPPLKSTHTV
                     LTRTQVVSLLKWYDQPHPTRPDRTIPGYDKAHALRTSRLCVAVATALGHPLSRLRQFE
                     AACLLHDMGRAGLDPVLFGRIWSWAKEQNIPTRPREWRARYPHTRYGRESQAFLAQYG
                     AALQARGLPLTTAVKDHIDMRLGFARRLHTYLRPVKTAIATVDIPWAPWMERIMLYYY
                     YPEKLEHAAAWTHQLAEILVACEQLEAYSNHRRGKDYYARAEESFAAAFAFLRQLAAQ
                     RIVSPQVMDIICRLTREGRFTTILRQARGGTLSAADQQFLQTVSC"
     gene            1933..5292
                     /locus_tag="VLA60_04835"
     CDS             1933..5292
                     /locus_tag="VLA60_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HSF08715.1"
                     /translation="MRWSGKQMFWWGALGALVLTIALIVLPLFLNPDYLKTLALQQIQ
                     RTFGSHVTVGHTSFALFPSPHFLVLDIVVQEQPESHAVFRAKSMSLDLGIGQLLMKKI
                     VVREFLVESPEIELRREADGEWRFLSYASNDSPIASATKFLVLGKVVVTDGKIIVIDE
                     SPREIVRGFVIEDVAFDSDTTHDGPSMASSFELSGKIRQTQELAPFRIKGELEARWAS
                     PVLSIASEPVGFEQIRFSGKVKADHLEINQLGEYLPNGQSLMAVPGTLSVKSQVTWVQ
                     NAKTSRLSLSHIDVSSSFIDLDGSANTEMLEDGHQMMGFSMRSSNVDLAMIRQYLPKT
                     WIPDDLLPVWETGEWGGGLNIAEARVTGSTREDVQTSVTGTFHLTDGYVRIPDWPTTD
                     HIQGTVVVEPDRMQLSGVHGIYDGIPVEVTEGVFLLKESGPWGDVGLQGPVPAEKVRQ
                     VVTQLGAPSDFGLLNAWKVSDGSGLLRLRFAGNLLESPGLKFQYGEFKPEGLVIQIPG
                     LPQPFTQGHGTITFSRESTVLEGLQGNVGAYPLAVNGTITYKGTSRFEPLTIQGGFAG
                     EDLFPVSGNRAASSGMRITGPLHASVTLTGPTRHPKLKGWIDGREAFMDVPSLLQKQA
                     GQEGRLEFDGQFHPGSRVHFERVELIMLPLRLRGQGTIRYRSEVTWEARFDSGPVYLG
                     VLPEGIRVLGDLVQSGILEVQLKGSGRGTDWTRWNTKGWVALTEGVVALRGVSDPISN
                     LFIRLKVDEDQLDLKRMEFRLKNSEAVITGFMKNWKTTPIASVLFDSPQFDIDLLIPK
                     EGRSAIRDGIEWLAAHGSLEGSVHIERPIYKALSGKKLSAVLKIHDNLVSVDKVQSMV
                     AEHATVGGRFFVHLPEGRPAAMRASFQAKDLPFEQVLSLFGEERRLITGTMSVRGMLQ
                     GHGRDDRGIIPTLNGSVKISLGEGYIRKGTIFPRILALLNLPQVLRGKVDLEETGFPF
                     TTVSSKLTVEDGVFSTKDLLIQSPIMKVSAAGRYDWNRDRLEGISAVSPFGAYSDILK
                     DIPLFGRIFSGDRKGIATAMFSIDGPLGDPEVKYLPMESVKTGLTGLAQLAFDVLKNT
                     LTLPYDLLNGSQEDAGSPPPDTGRSGVPGPL"
     gene            5400..6440
                     /gene="mtnA"
                     /locus_tag="VLA60_04840"
     CDS             5400..6440
                     /gene="mtnA"
                     /locus_tag="VLA60_04840"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HSF08716.1"
                     /translation="MIPTVEWKNNRVVILDQRALPGDVRFLDCTGYEEVAEAIRNLSV
                     RGAPAIGVTAALGIALGARQYADTHSEGFSAHMEEVCRTLAATRPTAVNLFWAVDRMT
                     RVMTGLSTSSIQDIQKRLQEEALAILEEDLRVNRAIGRFGAAIIHDGDHILTHCNAGA
                     LATAGYGTALGVVRAAWEQGKQIRVFADETRPVLQGARLTAWELQQDGIPVTVITDNA
                     AGALMRQRGIHCCVVGADRIAANGDVANKIGTYSVAVLAHAHGIPFYVAAPSSTIDMG
                     TADGNGIPIEERSPSEVTCLHSGRMIAPQGVEVWNPAFDVTPADLIAGIITERGIIPP
                     HALAQHFSSPSS"
     gene            complement(6453..8834)
                     /locus_tag="VLA60_04845"
     CDS             complement(6453..8834)
                     /locus_tag="VLA60_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08717.1"
                     /translation="MTKNRQKTSQPISPVQSVEKWEQAFDALTDHVMILDSSGTIVWT
                     NRAIRDQVQPHIGPLVGHHYRTPYYGTTIPQIPPPWETVLAGALSAVVETQFPTMPGD
                     FLVSCYPLFDSQGIQWGAISVVKDITERKRIDEALRKIAQSDPAPGSMAFLRFLVRDL
                     CQALNVPYAILAELESPARPQTHTVAIWSNGTFGENMPWTPPPAIFEQLLKVKGCHGD
                     NLESPLFPPDSPLGPWSIISYLGTALRGRNGQIIGILLVFSPVAIRNIHVAQSIIQLF
                     AARAAGELQRKKAEDALRDSEQRYRAIVEHAYDLIIETTATGECQYVTPNCQKVLGYE
                     AADILGRNFFDFIHPEERGPLSESFHSHLKTLQEIDMVCRLQTKQSEWRWFESHIHPF
                     RTSVGTVVGVIVTRDITEWKRLEDERLRATKLESVGLLAGGIAHDFNNILTSVFANIG
                     LAKMVTAKQSPPQGEKVIERLEAAEKACLRARDLTKQLLTFAKGGAPVKSTTSVASII
                     TDTVQFALRGSAVRCTTHLPDDLWAIEVDEGQISQVIQNLVINSDQAMPDGGTISVVA
                     NNCLVPRQTVLPLKPGKYVCVSVADQGIGIPDDHLQKIFDPYFTTKQKGSGLGLATTY
                     SIMKRHGGHVAVTSSLGNGTCFTLYLPASTSLPAKNDETPESLVQGTGKILVMDDEED
                     IQDVLGKMLEHLGFEVDFAEDGVKAVALYTQAMQDGHPYLATILDLTIPGGMGGKETL
                     RLIKDHDPDAKVIVSSGYSNDPVMAHAGQFGFSGFIAKPYNLLDLSKVLTQII"
     gene            complement(8891..9892)
                     /locus_tag="VLA60_04850"
     CDS             complement(8891..9892)
                     /locus_tag="VLA60_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSF08718.1"
                     /translation="METTGTGPSIPQANILIVEDEEGPREALKVILSPYFNLFTVDRA
                     EIAHQILKTHPIDLVTLDLKLPDLSGNDLLSQIRKDGKDVDVIIITGYGTLQSAIEAI
                     RHGVAAYILKPFNVSELLDTINKTLERRRRYSSLQAALQAFGNLWASGIDVQSALANI
                     KTLLAAKHPELVQHGSRVDFYAALLIEHLQLTPEEQTAIQLGAYLHDIGKIGLHDHLI
                     AGLHSPTEQDLELLKCHPTIGGKMVKGLPFHPCVEQIIRHHHEQYDGSGYPDGLIGEH
                     IPLSARIVGLANIFDHFVTGYGSQAAMPVPEAREKIRQEAGKSVDPNLADLFAKVVW"
     gene            complement(10118..10768)
                     /locus_tag="VLA60_04855"
     CDS             complement(10118..10768)
                     /locus_tag="VLA60_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08719.1"
                     /translation="MKTQANRFIMVCIVSLGVVGCQGAGQTVNFNPHAFSSTMKATTN
                     QHEDLTIVVEPFQDKRAQKTRLGSRTHFWGGATHFNAWNGNLGEGMADLALEYLQQEK
                     WQASRAPTSDPSAGGTPADVILTGDILVMEANAKSGFGFTDIEVKIRVAFEARNAVDG
                     STVRMVLGANGTDSVVTFDPKDVEVLTNHVAKDLFNQLFLDLTVKDRAFQLKSDKL"
     gene            11072..11449
                     /locus_tag="VLA60_04860"
     CDS             11072..11449
                     /locus_tag="VLA60_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08720.1"
                     /translation="MWKHNFLFRAEGAVPLEQTENELFHDTDPALDSSGLQMDKYISV
                     WLQGDGDETKPLVYTTVYVRTATLDPEKGVGFLQPLQGRTHQIKSMLSSEQKLYLRQW
                     LSSTYPPAWEESDDHFQSIFSET"
     gene            11567..12841
                     /locus_tag="VLA60_04865"
     CDS             11567..12841
                     /locus_tag="VLA60_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF08721.1"
                     /translation="MDLQTQVLIIGAGGGGAVLGLLLARKGIANIVLEQAAGPPQGLR
                     GEILQPNGQEILQRLGLLEQLPVHAYKPVRFFHFRRSGGTRLCTIDYAMLPPPFNRAL
                     VMWPHVVHHTILDALKRENPEGLRYGASFRSLLRNGKGITGVKADVGGSQVTIGAQVV
                     VGADGPFSEVRRALGIPTALHRYLDSYLVAMLESPTSLEEAQYYVGKKSILGIFPAAG
                     QKVYAFYMVSSDSLNQVKADGVSALREKWKTIAPELAPVFDSFLDWNQTAYMGTGRVR
                     AKTWVQDGAVVMGDAAHGMNPHASQGRMQAMVDAVTLAEVLDECHRTGNWSASNLKVF
                     ETQRRPQVTMLQRLADEEVFFWNTGNPLLGMLRDRVFSTMDKNPRLQYQVLTATAGLR
                     TTSPFGWMDRFQAAGLLPDPRAHQIPDHARSA"
     gene            12900..13664
                     /locus_tag="VLA60_04870"
     CDS             12900..13664
                     /locus_tag="VLA60_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08722.1"
                     /translation="MPTSILPSLELPEDLRETLEQYVIELQKDWGADLEGLLLYGSAA
                     RGDFIAGRSNFNFLVLVRALSVSGLQRAGQLHRKWGKHQIVAPLVMTQEELAQSRSLF
                     PLEYFQMQEHHVRLAGKDPFGAGHIDGQQLGWQCAHELLANVFRMRQRFVEGEGRAEA
                     IRALLMLSITSVLPCVRGILRVIGQPSKGKDVQILECLPHALQFEPTVLLEILQMKRG
                     LNSPGSLEWSKVYERYLQTLEGLCQRVQAVRQESGV"
     gene            13661..14395
                     /locus_tag="VLA60_04875"
     CDS             13661..14395
                     /locus_tag="VLA60_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HSF08723.1"
                     /translation="MTMSTRRLVGPLILVFVLAFSALSMGMTPQEQNLPTPLGYVSDY
                     AHLLDEEWHKQIRSVCKDLESSSGVEMLVVTLPSISPLSHAQEYASRLYEAWRIGTAQ
                     QERGILLLVAVKERQAVVVVGKNLLSVVTPQQLDEISLKHLRPMFRSGEYGINIYRSA
                     LSLSTLAARATIQKEKPKPQRSAAFWMNIAVVVLMVYALWRFTRPERRHPFPRWKRGE
                     YWGTGQGGFGGNFGGFGGGTGGQGLR"
     gene            complement(14600..15277)
                     /locus_tag="VLA60_04880"
     CDS             complement(14600..15277)
                     /locus_tag="VLA60_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3386 family protein"
                     /protein_id="HSF08724.1"
                     /translation="MEKNTEEKPTTEVQDDPKARELLQAAFAKTARWQPDFKGFQSDL
                     RINVNGKETKGTVTVKGPRDVTVVIDDTELQTWAQNQIGMIAVHRGPRTFEESDGKHT
                     LTLGGEEQHPLGQRITIHGDGMGSWYRVKDNRITQINRNMPQAAFTINVEDSAVTQDG
                     HYLTTRYTVYYFSPKDRSLKNVDSFSDTHIRIGNSDLPATRRIISYENGEIVVRSLTF
                     ENHKMLS"
     gene            15678..>16469
                     /locus_tag="VLA60_04885"
     CDS             15678..>16469
                     /locus_tag="VLA60_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HSF08725.1"
                     /translation="MNAAEQQLNDVLKLQRSEGHGWLIHLFKAMVVVGMLLPTFALPT
                     VWAEEVPDVVGLSQGAAESAITGAGLTVGSITTSSHATVPSGSVISQTPGAGTSVTAG
                     SAVNLVVSTGPAPVTVPNVVGSPQGAAQTTLTKAGLKVGTVTESSHATVASGRVISQT
                     PGAGTSVAAGSAVNLVVSTGPAPVTVPNVVGSSQGAAQTTLTNAGLKVGTVTTSSHAS
                     VPSGRVISQTPGAGTSVAAGSAVNLVVSTGPAVPNVVGLTQAAAQS"
BASE COUNT         3497 a         4062 c         4883 g         4027 t
ORIGIN      
        1 cgaccagata tcccgacgca ccgccgtatt ggtgatgtag accaccgcct gcgcggcttt
       61 ttcaaagacg gcaatggtat tgagttcttc cgcaccaagc ggtttctggg cgacgaggtg
      121 cagataggag ggcgaggcga aaaccgattg cgcctgtttc gggacgaacg cctctcccat
      181 ggcgctattc ggcaggagcc ccgtgacgaa cgcgacgaga agaatatgct tctgccattt
      241 tgcacaccgg cggtgtagtc gatcgtggac gtttgtccgg tctgaaagga tgccttgcgg
      301 tgtaaggtgc gatagcatac gaagatggtt cccgggccta ttctcccatg agctggacaa
      361 ccaggtttcg ggttcgtggt ctggtatcaa aatcgattaa gacgagttgt tgccaggtgc
      421 caagagttaa ggcaccattt acgaaaggaa tcgtcagcga aggtccctgt aactgaccgc
      481 gaagatggga gtggccgtta tcttcgccgg cattccgcgt attatgttct aactgcggat
      541 cggcgggtat cgtgcggtcc caaaaggttt tagtatcggc acggattccc ggctcatctt
      601 cgatgatcat gaccgaggct gtcgtatgtt gcacaaagag cgtcagcatt ccactctgta
      661 gacctgattc ggcgagcagg tcgtgaatcg gctttgtgag gttttcgatg gtcgaattgc
      721 cggtcatgga aatgttgagg tagtgggtga tgacggccat gggtctcctc agcacgagac
      781 ggtttgtaaa aattgttgat cggcggctga tagagtgccc cctcgggcct gtcgtaaaat
      841 agtcgtaaaa cggccttccc tggtgagccg gcagattata tccataacct gtggactgac
      901 gatccgctga gcggcgagtt gacggagaaa ggcgaaggcg gcggcaaaac tttcttctgc
      961 gcgtgcgtag taatctttgc ctcgccggtg attgctatag gcttccagtt gttcgcaggc
     1021 gacgaggatt tccgcgagtt ggtgcgtcca ggcggcggcg tgttcgagtt tttccggata
     1081 gtaatagtac aacatgatgc gttccatcca gggcgcccac gggatgtcta cggtggcgat
     1141 tgcggtcttt accggacgaa gataggtgtg gaggcgtcgg gcaaatccca atcgcatatc
     1201 gatgtgatcc ttgaccgcag tggtgagcgg caggcctcgg gcttgtaagg cagctccata
     1261 ttgtgcaagg aaggcctgag attcgcggcc atagcgggtg tgggggtagc gggcccgcca
     1321 ttcccgtggc ctggtcggga tgttctgttc ctttgcccac gaccagatcc ggccgaacag
     1381 cacgggatca agtccggccc gccccatatc atgcagcaga caggcggctt caaattgacg
     1441 aaggcgggag agtggatggc ctaaggctgt ggcgacggcc acacacaggc gtgaggtgcg
     1501 cagcgcatgc gccttgtcat agccgggaat cgtacgatcg ggcctggtgg gatgcggctg
     1561 gtcgtaccat ttcagcagcg agaccacttg cgtacgggtg agaacggtgt gggtggactt
     1621 caatggtggc cgggacacga cggctagaat ggtacaatcc tcatgacgaa acgcgtaagg
     1681 attatggagt gttgaaccgt ggggcaggat atcccaggcc tggggaggtg tcaagaaacc
     1741 gatggcgttc tccatatggg acgggcggcc ggtcatggat cctctccaat ggggtcggtc
     1801 ccgggagcat cggaatcatt cttacatttc acatggattt cggtatgatg caggacaggt
     1861 tttcatggtt cctatccctg acaccacaca tacaacggtt tgggtttctg gtaatccgaa
     1921 gagggtgatc tagtgcgatg gtccggcaaa cagatgttct ggtggggggc actcggggcc
     1981 ctggttctga ccattgccct gattgtgctt cccttgtttt tgaatccgga ttatctgaag
     2041 actctggcgc tgcagcaaat tcaacggacg tttgggtccc acgtgaccgt ggggcatacg
     2101 tcctttgcct tatttccctc tccgcacttt ttggtgttgg acattgtggt tcaggaacag
     2161 cctgagtcgc atgcggtgtt tcgcgcgaaa tccatgagtt tggacctggg gatcgggcag
     2221 ttgctcatga aaaaaattgt ggttcgtgaa ttcctggtgg agtctcccga gattgaattg
     2281 cgccgtgagg ccgacggcga atggcgtttt ttaagctatg ccagcaacga ctctcccatt
     2341 gcgtcggcga cgaaatttct ggtgctggga aaggttgtgg tgacggacgg taaaattatc
     2401 gtcattgatg aatcgcctcg tgaaatcgtt cgggggtttg tcattgaaga tgtggccttc
     2461 gactctgaca ccactcatga cggcccttct atggcgtcat ccttcgaatt gtctggaaaa
     2521 atccggcaga cgcaggagct ggcgccgttt cgcatcaagg gagaactcga agcccgttgg
     2581 gcatccccgg tgctgtctat tgccagcgag ccggttggtt ttgagcagat caggttctcc
     2641 ggtaaggtga aggccgatca tctggagatc aatcaactgg gagaatatct gcccaatggc
     2701 caatcgctca tggccgtgcc gggcacgctt tcggtcaaat ctcaggtgac ctgggttcag
     2761 aatgcgaaaa cctcccgact ctctctctcc catatcgacg tatccagttc atttattgat
     2821 ctggatggtt cggccaatac ggaaatgctg gaagacggac atcagatgat ggggttctcc
     2881 atgcgctcct cgaatgttga cctcgccatg attcgacaat atctgcccaa gacgtggatt
     2941 ccggatgatc tgcttccggt gtgggagaca ggcgaatggg gaggcgggtt gaacattgcc
     3001 gaggcacggg tcaccgggtc cacgcgtgag gatgtgcaga cgtcagtcac cggcacgttt
     3061 catttaaccg atgggtacgt tcgtattccc gattggccca ccaccgatca tatccagggg
     3121 acggtggtcg tggaaccgga tcgcatgcaa ttgtccggcg tgcacggcat ctatgatggt
     3181 attcccgttg aggtgacgga aggcgtcttc ttgttaaaag agtcaggccc gtggggggat
     3241 gtgggccttc aagggcctgt gccagcggag aaagttcgac aggtggtgac gcagttggga
     3301 gcgccatccg actttggact gttgaatgcc tggaaggtct cggatggcag tggcctgctg
     3361 cgtctccggt ttgccgggaa tctgttggaa tcgcctggat tgaaatttca gtatggcgag
     3421 tttaaacctg aagggctggt gatacagatt cccgggctgc ctcagccttt tacgcaaggc
     3481 cacggtacca ttaccttttc ccgtgagagt acggtgttgg aaggtctaca aggaaacgtg
     3541 ggcgcctatc cgttggccgt gaatgggacc atcacttaca aggggacctc tcgctttgag
     3601 ccgctcacca ttcagggcgg gtttgccggc gaggacctct tccccgtttc cggaaaccgg
     3661 gcggcatcat cagggatgcg gatcaccggt ccgcttcatg cctcggtcac tctcacgggg
     3721 ccgacccgtc atcccaaact gaaagggtgg attgacgggc gagaggcctt catggatgtg
     3781 ccctctcttt tacagaagca ggccggtcag gagggacgcc ttgagtttga tggacagttt
     3841 catcccggca gccgtgttca ttttgaacgt gtggaactga tcatgcttcc gcttcgtctt
     3901 cgcggacagg ggaccatccg gtatcgctcg gaggtcacat gggaggcgag gtttgattcc
     3961 ggtccggtgt atttaggcgt gctgccggaa gggattcgtg tcctgggaga tctcgtccag
     4021 tcgggcattc tcgaggtgca attgaaaggc agcgggcggg gcaccgattg gacccggtgg
     4081 aataccaaag ggtgggtggc gttgaccgaa ggcgtggtgg cgcttcgcgg ggtcagcgat
     4141 cccatttcga atctgtttat tcgattgaaa gtggatgagg atcagcttga tttgaaacgc
     4201 atggagtttc gcctgaagaa tagtgaagct gtcatcaccg gatttatgaa aaactggaag
     4261 accacaccaa tcgccagtgt cctgtttgac tcgccgcaat tcgatattga tttgctgatt
     4321 cctaaagaag gacgatcggc catccgggat gggatcgagt ggttggcggc gcatggttcc
     4381 ctggagggtt cggtgcatat cgaacggccg atctataagg cgctttccgg caaaaaatta
     4441 tcggccgtgc tgaagattca tgacaacctg gtctcggtgg ataaagtcca atcgatggtg
     4501 gcggaacacg ccaccgtcgg ggggcgattc tttgtccatc tcccggaagg gcggcccgcc
     4561 gcgatgcgcg cgtcattcca ggcgaaggat ttgccttttg agcaggtctt gagtcttttt
     4621 ggcgaggagc gtcgattgat taccggtacc atgtcggtgc ggggcatgct tcagggtcat
     4681 ggacgggatg accgcggcat cattcccact ctcaacggga gtgtcaaaat ctctctcggc
     4741 gagggctata tccgcaaagg cacgatcttt ccacggattc ttgcgctctt gaatctacca
     4801 caggtccttc ggggaaaagt ggatcttgaa gagaccggat ttccgtttac gaccgtgagc
     4861 agtaagctca ccgtggaaga cggggtgttt tccacgaaag atttgttgat acagagtccc
     4921 atcatgaaag tctctgccgc cggaaggtat gattggaaca gggaccggct tgaagggatt
     4981 tccgccgtca gtccgtttgg ggcctattcg gatatcttga aagatatccc tctctttgga
     5041 cgaatttttt ccggagatcg gaaaggcatt gccacggcga tgttttcgat tgacggacca
     5101 ttgggtgatc cggaggtcaa gtatctgccc atggaatccg tgaaaaccgg actgaccggt
     5161 ttagcacagt tggcctttga tgtattaaaa aatacgttga ccctgcctta tgatttatta
     5221 aatggttctc aggaggatgc gggttctcct ccaccggata ctggacgatc cggcgtgccc
     5281 ggtcccttat aagtcttttg tctgtgccga cggatgtgtc gggatggaca ttcagagaag
     5341 gcggtgaggg ccgtgcacca tttgagtcgg ttttaacatc tcgtgtgaaa ggattgagga
     5401 tgattcccac agttgaatgg aaaaataatc gtgtcgtgat tctggatcaa cgtgccctcc
     5461 ccggagacgt gcggtttctt gattgtacgg gatatgagga ggtggctgaa gccattcgca
     5521 atttgagtgt ccgcggcgcc ccggccatcg gggtgacggc ggctttgggc atcgccttgg
     5581 gggccaggca atatgcggac actcactctg agggattttc cgcccatatg gaggaggtgt
     5641 gccgcacgct ggccgcgacg cgtccaaccg cggtgaattt attttgggcc gttgaccgga
     5701 tgacccgcgt gatgacgggt ctctcgacgt catccattca ggatatccag aaacgattac
     5761 aggaagaagc cctggcgatt ttggaggagg atcttcgggt caaccgggcc attggcaggt
     5821 ttggcgccgc gatcattcat gatggagacc atattctcac gcattgtaat gccggagctt
     5881 tagcgacggc aggatatggg acggcattag gcgtggtccg ggcggcctgg gagcagggca
     5941 aacagattcg ggtctttgcc gatgagacgc gcccggtcct tcagggggca cgcctgacgg
     6001 cctgggagct tcaacaggat ggtattcctg tgacggtgat tacggataat gcggccgggg
     6061 ccctgatgcg gcagcgcgga atccactgtt gcgtcgtggg ggctgaccgg attgcggcca
     6121 atggcgatgt ggccaataag atcggcacgt attccgtggc ggttttagcg catgcccatg
     6181 gcattccttt ttatgtggcc gcaccttcat cgaccattga tatggggacc gcggatggca
     6241 acggcattcc cattgaagag cggagcccgt cagaagtgac ctgcctgcat agtggccgga
     6301 tgattgctcc tcaaggtgtg gaagtctgga atccggcgtt tgacgtgacc ccggccgatc
     6361 tgattgcggg cattattacc gaacggggga ttattccccc gcatgcctta gcccaacatt
     6421 tttcgtcccc atcttcctga tcgaatgatc cgtcagatga tctgagttaa gactttagac
     6481 agatcgagga gattatacgg cttggcaata aagcctgaga atccgaattg gccggcatgg
     6541 gccatgaccg ggtcgttgga atacccgctg gagacgatga ccttcgcatc cggatcatgg
     6601 tctttgatga ggcgcaacgt ctccttgcct cccattcctc cgggtattgt gagatcaagg
     6661 atggtggcca aatacgggtg gccgtcctgc atggcttggg tatagagcgc cacggctttc
     6721 actccatctt cggcaaagtc gacctcaaat cccaggtgct cgagcatttt ccctaagaca
     6781 tcctggatgt cttcttcatc atccatgact aaaatttttc ccgtgccttg cacgagactt
     6841 tccggtgttt catcgttttt ggcgggcagg gaggttgagg cggggagata gagggtaaag
     6901 catgtgccgt tgccgagtga agaggtgacg gccacgtgcc ctccatgccg tttcatgatg
     6961 gaataggtcg tggccaggcc cagtccgctc cctttttgtt tggtcgtaaa gtagggatcg
     7021 aagatctttt gaagatgatc gtcagggatg ccgatgccct ggtcggcgac ggacacacac
     7081 acatacttgc cgggttttag aggaagcacg gtctgacggg gtaccaggca attgttggcg
     7141 acgacgctga tggtgcctcc atccggcatg gcctggtcgc tattgatcac cagattttga
     7201 attacctgac tgatttgtcc ttcatcgact tcgattgccc agaggtcatc ggggagatgc
     7261 gtggtgcacc ggacagccga ccctcgtaag gcgaattgca ccgtgtccgt aataatagag
     7321 gccacagagg tcgtgctttt gaccggggcg ccgcctttcg caaaggtgag cagctgcttg
     7381 gtgagatccc gcgctcgcaa acaggctttt tccgcagcct ccagtcgttc aatgactttc
     7441 tcgccctgag gtggcgactg tttcgcggtc accattttgg ccaggccgat attggcaaac
     7501 acggacgtga gaatattatt gaaatcatgc gcaatgccgc cggccagtaa gccaaccgac
     7561 tcaagtttgg tggcccggag tcgttcatct tccaggcgtt tccattccgt gatgtctcgt
     7621 gtcacgatca cccccacgac cgtcccgacg ctggttcgga aggggtggat gtgactttca
     7681 aaccatcgcc attcggattg tttggtttgc aaccggcaca ccatatcaat ttcctgaaga
     7741 gtcttgagat gagaatgaaa cgactcggat aacggtcccc gttcttcggg atggataaag
     7801 tcaaagaaat ttcttcccag tatgtcggcc gcctcatagc ccaggacttt ttgacaattc
     7861 ggggtgacgt actgacattc acccgtcgcg gtggtttcga tgattaaatc ataggcatgt
     7921 tcgacgatgg cccgatagcg ctgctcacta tcccgcaagg catcttcggc ttttttacgt
     7981 tggagttcac cggccgccct ggccgcaaac agttgaatga tggattgcgc cacatggatg
     8041 ttgcgaatgg ccaccggact aaagaccagc aggatcccga taatttgtcc gttccggcca
     8101 cggagggctg ttcccaaata gctgatgatc gaccatgggc cgagggggga gtcaggagga
     8161 aagagcgggg attccaaatt gtccccatgg catcctttga ctttcagcag ctgctcaaaa
     8221 atcgcgggag gaggggtcca cggcatattt tctccgaaag tcccattgga ccaaatggcg
     8281 accgtgtggg tctgcggccg ggccggggat tccaattccg ctaatatggc atagggcaca
     8341 ttcagggcct gacagagatc tctcaccaaa aaccgcaaaa acgccatgct gccgggagcc
     8401 ggatcgcttt gggcaatttt ccgcaacgct tcatcaatcc gtttgcgttc ggtaatatct
     8461 ttgaccaccg aaatggcacc ccattgaatg ccctgggagt cgaatagggg atagcaggag
     8521 acgaggaaat cccccggcat cgtcgggaac tgtgtttcca ccaccgcaga caatgcgccg
     8581 gctaacaccg tttcccaggg aggagggatt tggggaatgg tggttccata gtagggggtg
     8641 cggtaatgat gtccgactaa tgggccgatg tgcggttgga cctggtcacg aatggctcga
     8701 ttggtccaga caatcgtccc tgaactgtct aagatcatga catgatccgt cagggcatca
     8761 aacgcctgct cccatttctc gacggactgc accgggctga ttggttgtga agtcttttga
     8821 cgattcttag tcatagtgtt tcagtggtgc cggcttatgg tggctcatgt gtctcccgcc
     8881 tcgagtcccg ttaccagacg actttggcga acaaatcggc cagattcgga tcgacacttt
     8941 tgccggcttc ctgacgaatt ttttcccggg cttcgggcac gggcatcgcc gcttgggacc
     9001 catagccggt cacgaagtgg tcgaaaatat tggccaggcc gacaatgcgt gccgataagg
     9061 gaatgtgttc accgatcaat ccatccggat agccggaccc atcatattgt tcatggtgat
     9121 ggcggatgat ctgttcgacg catgggtgaa atgggagacc cttcaccatc ttccctccga
     9181 tggtgggatg acatttgaga agttccaggt cttgttccgt cggggaatgc agtccggcaa
     9241 tgaggtgatc atgaagaccg attttcccga tgtcatggag gtaggccccg agttgtatgg
     9301 ccgtctgctc ctccggggtt aactggagat gttcaatgag cagcgcggca taaaaatcca
     9361 cccggctgcc atgttgaacc agttcaggat gtttggcggc caggagggtt ttaatattgg
     9421 ccagggcgga ttggacatcg atcccggacg cccaaaggtt tccaaatgcc tgaagcgctg
     9481 cttgcaggga ggaatatcgg cggcggcgct cgagggtttt attgatggtg tcgagcaact
     9541 cggacacatt gaacggtttg aggatatagg cggccacccc gtggcggatg gcttcaatgg
     9601 cagattggag tgtgccatat ccggtgatga tgatgacatc gacatctttt ccgtcttttc
     9661 gaatctggga aaggagatcg tttcccgaca gatcagggag ttttaaatcg agggtgacga
     9721 gatcaatggg gtgagttttg agaatttgat gcgcgatttc ggcccgatca accgtaaaga
     9781 gattgaaata gggagaaagt ataaccttta acgcctcgcg cgggccttcc tcgtcctcca
     9841 caattaaaat attcgcctga gggatagagg gtccggtacc agtagtttcc atcaacgact
     9901 ccttttccaa gtgaagcgac ccatgaagac agtaaaaaaa ataaatagtc catgagacct
     9961 tgaacggtcg cttctgagga tgaagtcgga gatcatacct tctccgtcga ggcttgcatg
    10021 acgtttagta gcgattcacc tcctgaggac cataatggtt cgtgctcgta gcatgtgaag
    10081 aggaaagaac gggagatagg gcacaagagt cgtcatttta taatttatct gattttaatt
    10141 gaaacgcccg atctttgact gtcagatcta gaaataattg gttgaacagg tctttggcga
    10201 catgattggt gagtacctcg acatccttgg gatcgaatgt gaccacgcta tcggttccat
    10261 tcgcaccgag caccatccgg acggtgctgc catccaccgc atttctcgct tcaaaggcta
    10321 cacgtatttt gacctcaata tccgtaaagc cgaagccgga tttcgcattc gcctccatga
    10381 ccaaaatatc gcctgtcaga atgacatcgg ccggtgtacc tcctgcggag gggtcagacg
    10441 ttggtgcccg cgaggcctgc catttttcct gttggaggta ttccagggca agatccgcca
    10501 tgccttcgcc gaggtttcca ttccaggcat taaaatgcgt cgcacctccc caaaaatgtg
    10561 tgcgggaacc cagacgagtt ttctgtgctc gtttatcctg aaacggttca accacaatgg
    10621 tcagatcttc gtgttggttg gttgtggcct tcatcgtgga cgaaaaggca tggggattaa
    10681 aattcaccgt ctgccccgcc ccctgacatc cgacgactcc caggctgaca atacacacca
    10741 tgataaacct gttggcttgt gtcttcactc gtggaacctc catgtataga aagtggatat
    10801 cagatgatcg tctagggtgt gacgggagag ggatgtggct gccgataccc tattgtgtcc
    10861 gggccacaca tccatccgaa ttttagacag cgtaagaata ataaacctgc agaatttaat
    10921 ctagtcttgc gttgattttc cgtgatgagc ccgcatgaat gattccgata taccgccacc
    10981 tgatgatgat ggcatgatct gtcttgacat tgtcgtaaac cgcttgacac tatcatacct
    11041 ggtttgattg ttaatccaaa ggaaattctt catgtggaag cataattttt tgtttcgagc
    11101 tgaaggggcg gtgcccctgg agcagaccga aaacgagttg tttcatgaca cggacccggc
    11161 cctggatagt tcgggcttac agatggataa atatatttcc gtctggctcc aaggggatgg
    11221 cgatgagacc aagccactgg tctatacgac ggtgtatgtt cgaacggcca cgttagaccc
    11281 cgagaaaggg gttggattcc ttcagccgct tcaaggacgg acccaccaga tcaaaagcat
    11341 gttgtcctca gagcagaaat tgtatcttcg gcaatggttg tcgagtacct atccaccagc
    11401 ctgggaagag tctgacgatc actttcaatc cattttctcc gaaacctgat ctctccattc
    11461 ccgccggagg gtgatcggtt tctgagatcg gcatcggtga atttcagctt cccgctcgcc
    11521 ctggtgtcct ggtcggcggc gaatgtgtat tcgtagggtc ttaactatgg atttacagac
    11581 acaggttctg atcataggcg ccggtggcgg gggagcggtc ctgggacttc tcttagcccg
    11641 aaaaggaatt gccaatattg ttctggaaca ggctgccggg cccccacagg gcctgcgtgg
    11701 agaaattctt caacccaatg ggcaggaaat ccttcagcgg cttggtttat tagaacagtt
    11761 gccggttcat gcctataaac cggtcagatt ctttcatttc cggcgaagtg gaggaacgag
    11821 gctctgtacc attgactatg cgatgttgcc cccgccgttc aatcgggctt tggtgatgtg
    11881 gcctcatgtc gtgcatcaca ccattctcga cgcgctgaag cgggagaatc cggagggact
    11941 tcggtatggg gcaagttttc gttctctgct ccggaatggc aaaggtatca caggcgtcaa
    12001 ggcggacgtt gggggcagcc aggtgacgat tggggcccag gtggtggttg gcgcagacgg
    12061 accgttttcg gaggttcgga gagcccttgg cattcccacc gcgctgcatc ggtatctcga
    12121 cagttatctg gtggccatgt tggaatcacc aacatcacta gaggaagcgc agtactatgt
    12181 ggggaaaaaa tctattctgg gaattttccc cgctgccggg caaaaggtct atgcatttta
    12241 tatggtttcg tcggattccc tgaaccaggt caaagccgat ggggtatcgg ctcttcgaga
    12301 aaaatggaaa acgattgcgc cggaacttgc cccggtcttt gactccttcc tggattggaa
    12361 tcagacggcc tatatgggaa ccggaagggt ccgggcgaaa acctgggttc aggatggagc
    12421 ggtggtcatg ggtgatgccg cccatgggat gaatccacat gcctcccagg gacggatgca
    12481 agcgatggtg gacgcggtta ccctcgcgga ggttcttgat gagtgtcatc ggacgggaaa
    12541 ctggtcggcg tcgaatctta aggtatttga aacgcaacgg cgtccccagg tcaccatgct
    12601 gcaacgtctg gctgatgaag aggtcttttt ctggaatacc ggcaatcctc tcctggggat
    12661 gttgcgagac cgcgtcttct caacgatgga taaaaatccg cgtttgcaat atcaagtgtt
    12721 gacggccaca gccgggctcc ggaccacctc accctttggt tggatggatc ggtttcaagc
    12781 tgcgggcttg ttaccggatc cccgggccca tcagattccc gatcatgcac ggtctgcgta
    12841 attaaccttg aagtccattt tcgtcactca tcctttttcc aatatttggc tcgtatccta
    12901 tgccgacatc aatcttgccg tcactcgaac ttcccgaaga tctgcgggaa acactcgaac
    12961 aatatgtcat agagcttcag aaggactggg gcgctgattt agaagggctt cttctgtatg
    13021 gcagcgcggc ccggggagat tttattgccg gccgatctaa tttcaacttt ttggtcctgg
    13081 tccgggcact atcggtgagc gggctccagc gtgcagggca attacatcgc aaatggggga
    13141 agcaccaaat cgtcgcgcct cttgtgatga cccaggagga attggcccaa tcccgttctc
    13201 tgtttccgtt ggaatatttt caaatgcaag agcatcatgt gcgcttggcg ggcaaagacc
    13261 cgttcggcgc agggcatatt gatggtcagc agttgggatg gcaatgtgcg catgaactcc
    13321 tggcgaatgt gtttcggatg cggcaacggt ttgtcgaagg agaaggacga gccgaggcga
    13381 tccgggcctt gctcatgctt tccattacct ccgttctccc gtgtgtgcga ggtattctgc
    13441 gggtcatagg ccaaccctca aagggaaaag acgtccagat tcttgaatgc cttccccatg
    13501 ccctgcagtt tgaacccacg gttctcctcg aaatcctgca aatgaagcga ggcctgaata
    13561 gtccgggttc ccttgaatgg tccaaggtgt atgagcggta tcttcagacg ttggagggtt
    13621 tgtgtcaacg cgttcaggcg gttcgtcagg agagcggcgt atgaccatgt ctactagaag
    13681 gcttgtcggt ccgctcattc tagtgtttgt gctggctttc tctgcactct caatggggat
    13741 gactccccag gaacaaaacc tcccgacccc gttggggtat gtgagtgatt atgcccacct
    13801 gctcgatgag gaatggcaca aacaaattcg atccgtgtgc aaagatcttg aatcctcttc
    13861 cggtgttgaa atgctggtgg tgaccctccc gagcatcagc cctctttccc atgcccagga
    13921 gtatgcctcc cgcctctatg aagcctggcg gattgggacg gcgcaacagg agcgtggcat
    13981 cctgttactg gtggctgtca aagaacgcca ggcggtggtg gtcgtcggga agaacctttt
    14041 gtcggtcgtg actccccaac agttggatga gatttctctc aagcatttgc gtccaatgtt
    14101 tcggtctgga gagtatggaa tcaatatcta ccggtctgcc ctatcccttt ccacactggc
    14161 cgcgcgggct actattcaga aggagaaacc caagcctcag cgatcggccg ctttttggat
    14221 gaatatcgcg gtggtggtgc tgatggttta cgcgctctgg cggttcaccc gtcctgaacg
    14281 gcgtcatccg tttccccgat ggaaaagggg agaatattgg ggaaccgggc aggggggatt
    14341 cggaggaaac tttggaggat ttggcggagg aacgggaggc cagggcttac gttgaaggag
    14401 ctgggctgag ccctctcctt tccttaggca tcgtgaaata agtggggtgg taatgggagc
    14461 accgtttccc cgtcctgcat ttgcagaatt ttcacacgtt ctctaaaaaa tcttttctct
    14521 tcgagagtag gggtgggaag atccatcgca taaaatctat caaccccagg ccaacttcat
    14581 gttggcctgg ggttgtgcgt tatgacagca ttttgtgatt ttcaaacgtg agggatcgca
    14641 caacgatttc cccgttttca taggaaataa tgcgtcgggt cgccggaagg tctgagttgc
    14701 ctatcctgat atgtgtatcc gaaaaactgt cgacattttt cagtgaccgg tctttcggag
    14761 aaaaataata gaccgtatat cgggtggtga gataatgtcc atcctgggtg actgcactat
    14821 cttccacgtt gatggtaaaa gccgcctgtg gcatgtttcg attgatttgg gtaatgcggt
    14881 tgtctttcac ccggtaccag gaacccattc catctccatg aatggtgatc cgttgaccca
    14941 aagggtgctg ttcttcaccc cctaatgtca gggtatgttt cccgtctgat tcctcgaacg
    15001 ttctcggccc ccgatgcacg gcaatcattc caatctggtt ttgggcccac gtttgtaatt
    15061 ccgtatcatc gatgaccacc gtcacatccc gcggcccttt tacggtcacg gtccctttgg
    15121 tttcttttcc gttcacatta atgcgcaggt cggactgaaa gcctttgaag tctggttgcc
    15181 acctggcggt cttggcaaag gcggcttgta gcaactcgcg ggcttttgga tcatcctgga
    15241 cctcggtggt tggtttttct tctgtattct tttccatgtc gaagactcct tctcctgtga
    15301 tgtcgtgtag gtgatcagac tgattaagcg aacactgagg accattatag ggatctcagg
    15361 agaagtggac aaccgttcag atggagcgag gtccagagga ttcctcagtc tctttagtcg
    15421 tatcggaaac ttggaacaat taaatttttc aaaacccctc tgtcagaatc atccttgcta
    15481 aaggtttaac tctttcccca gatgcatata aaagcataaa aatgggaaga taaaggtaca
    15541 ttgaaaagag ggtggtgttc ggctaggctg catccataat tttttatctg aggggagaat
    15601 tccggagata ggtgtccctg tttttcatcc cttttaaaag atgattcgct tgcttccacc
    15661 cccaagaggc attgcgcatg aacgccgcag agcaacagct taatgatgtc ctgaaactcc
    15721 aacgaagtga aggccatggt tggctgatac atctttttaa ggccatggtt gtggtgggta
    15781 tgttgttacc cacctttgcc ttgccaacag tatgggccga agaagtcccc gatgtggttg
    15841 ggttgagtca gggagcggcg gagtcggcca ttacaggggc gggcttaacg gtggggtcca
    15901 tcacgaccag cagtcatgcc acggtgccgt cggggagtgt catcagtcaa acgccggggg
    15961 ccggcacgag tgtgacggcg ggcagtgcgg tgaacctggt ggtgtcgaca gggccggcgc
    16021 cggtgacggt gccgaatgtg gtagggagcc cgcaaggggc ggcgcagacc acgctcacca
    16081 aggcgggcct gaaggtggga accgtcacgg agagcagtca tgccacggtg gcctcggggc
    16141 gggtcatcag tcaaacgccg ggggccggga cgagtgtggc ggcgggcagt gcggtgaacc
    16201 tggtggtgtc gacggggccg gcgccggtga cggtgccgaa tgtggtgggg agctcgcaag
    16261 gggcggcgca gaccacgctc accaatgcgg gcctgaaggt gggaaccgtc acgaccagca
    16321 gtcatgcgtc agtgccgtcg gggcgggtca ttagtcaaac gccgggggcc gggacgagtg
    16381 tggcggcggg cagtgcggtg aacctggtgg tgtcgaccgg gccggcggtg ccgaatgttg
    16441 taggcctaac ccaggccgcg gcgcaatct
//
LOCUS       DATIAL010000154         3501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_456991, whole genome shotgun sequence.
ACCESSION   DATIAL010000154 DATIAL010000000
VERSION     DATIAL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456991"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1392
                     /locus_tag="VLA60_04890"
     CDS             370..1392
                     /locus_tag="VLA60_04890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF08726.1"
                     /translation="MTNMNSSEGPSPALFFQTVNGHMRTAALKSAIELELFSAIAEGH
                     CTPKALAHRCGGSERGLRMLGDYLTVGGFLTKQGEEYQLTADSELFLTKTSAAYLGPT
                     LNFMLSSPLVEGFKQLTGAVRKGGTVVGEKGTLAPEHPDWVTFARSMVPLMKGPAEWI
                     ANWVKQEAPGTRKVLDVAAGHGVFGIEIARTIPEADITAQDWPNVLMVARENAQAAGI
                     TKRFHELPGSAFEVEFEKDYDVILLTNFLHHFDVPTCDAFLRKVNGSLKPGGSVITLE
                     FIPNEDRISPAPMAEFCLIMLATTPAGDAYVFSEYDRMFRHAGFGESIMKDIPASNER
                     VIITKK"
     gene            1668..2093
                     /locus_tag="VLA60_04895"
     CDS             1668..2093
                     /locus_tag="VLA60_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF08727.1"
                     /translation="MPVQVYGVNHVVVEVDDAQKAVEFYADVFNLEMLRGGEGAAWCK
                     MGEHQFLAIFEVKTLQPDRTKHFGIMVRDDAQVREVREKITKKYGLEVHPDFRCDFRD
                     PWGNRIQVGDLHDESLVWLLPYREVQDSGITFGPTDIGS"
     gene            2423..2632
                     /locus_tag="VLA60_04900"
     CDS             2423..2632
                     /locus_tag="VLA60_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSF08728.1"
                     /translation="MPKYEIIIYWSQEDEAFIAEVPELTGCAADGPTYQEALSNVKIV
                     IREWLETAKELGRPIPQPKGRLVFA"
BASE COUNT          951 a          757 c          907 g          886 t
ORIGIN      
        1 gtcgtcgtac ggaagtatgc atttagcttt ggaacccagg gccatttgag ggagcgtggc
       61 aattcccacg catttattat aggagagctc ttaagcaaat cgtgtaggtt gtttgagcaa
      121 cgggaatttc cgaggaatcc ccctcctttg taaggagggg caaggggagg tagaaaggcc
      181 agatgccaat ttgtcgcgag ggctgttggt ccactgatat gagcacatgg ttgaatctct
      241 accccaccta acctccccct acaaggggga ggaagttgta ggcagaggac ttaagcattg
      301 tcccaggggt atacccacac taaatccgga agggccataa taagataatc caaaagacta
      361 ggagaacaca tgacgaacat gaattcatcc gaaggacctt ctccggcatt gttttttcaa
      421 accgtcaatg ggcatatgcg cacggcagcc ctgaaatccg ccatcgaatt ggaactgttt
      481 tcggccattg cggaaggcca ctgcaccccc aaagccttag ctcaccgttg tggaggttct
      541 gagcgagggc tgagaatgtt gggagattat ttgacagtgg gaggttttct gaccaaacag
      601 ggggaagaat atcagttgac tgctgattcg gaattatttt taacgaaaac gtcggctgcc
      661 tatttagggc cgactctgaa ttttatgttg tcatcgcctc tcgtggaagg attcaaacag
      721 ctcactggtg ccgttcggaa agggggaaca gtggtcggag agaaaggaac cctggcaccg
      781 gaacatccgg attgggtgac gtttgcgcgg tctatggttc cgttaatgaa aggtccggct
      841 gagtggattg ccaattgggt gaagcaggaa gccccgggta cccggaaggt tttggatgtg
      901 gcagccggtc atggggtgtt cggcatcgaa atcgcccgca caatcccgga ggcggacatt
      961 acggcacagg attggcccaa tgtgttgatg gtggccagag agaatgcgca ggcagccggg
     1021 atcaccaaac gatttcatga attgccgggg agtgcctttg aggtggaatt cgagaaagac
     1081 tatgacgtga tccttctcac gaattttctg catcacttcg atgtgcccac ttgcgacgct
     1141 tttttgagga aagttaatgg ctctctcaag ccgggagggt cggtgatcac gctggaattt
     1201 attcctaatg aagatcgcat ttctccggcg ccgatggcgg agttctgctt gattatgttg
     1261 gccacgactc cggccgggga tgcgtatgtc ttcagtgaat atgaccgcat gttccgccat
     1321 gccggatttg gcgagagcat catgaaggat atccctgcat cgaatgaacg ggtgatcatt
     1381 acgaaaaaat aggggggtct tccgacattc gcatgggcaa atattaaagg gtgaacgtta
     1441 gagagtcccc ctcctttgta aggaggggca aaggggaggt agcctctgcg gggccttcaa
     1501 tgattgccca gtgatgtttt actgatagcc atcggactct acctcaccta ccctctcctt
     1561 acaaaggaga ggaattcgaa gctgcctcat gtcgaaagaa gagcacgaga atgagagtga
     1621 ccgacggaaa agcctgatga accgaatagg gaaaaggaga aaccgttatg ccggtacaag
     1681 tctatggagt caatcatgtg gtggttgaag ttgacgatgc gcaaaaagcc gtcgagtttt
     1741 atgcggatgt cttcaatctt gaaatgctac gaggcggtga gggggctgcc tggtgcaaaa
     1801 tgggggagca tcagtttttg gcgatcttcg aagtgaaaac ccttcaaccc gatcgcacca
     1861 aacatttcgg catcatggtt agggatgacg cccaggtgcg tgaagttcgg gaaaaaatta
     1921 ctaagaaata cggactggag gtgcatccgg attttcgatg tgatttccgg gatccctggg
     1981 gaaatcgaat tcaagtcgga gatctgcatg atgaatcctt ggtctggctc cttccctacc
     2041 gggaggtcca agatagtggg attacgtttg gcccaacaga tataggctcc taatctcctt
     2101 gcctcttcaa gaagaatttg cctagaccgt ttaaattcat tgatattgtt aggtaaaaat
     2161 gctgcgataa tgtccaaata cgaaaaggtt cttttccaaa tactgcgtgg tacctcaggt
     2221 gcccacattc cgtttgaagg tttgcggaat taatttcttc accttcaatt taaagaacgc
     2281 atcaaggggt ctcatcatat tttaaccaga gaaaatgtgg gagaaattct caatcttcaa
     2341 cccaaagggt cacaggccaa accgtatcaa gtcaagcaag ttcgtctggt cttgatcaag
     2401 taccacttag gaggatccgt cgatgccgaa atatgaaata atcatctatt ggagtcagga
     2461 agatgaggct tttatcgccg aggttcccga attaacgggg tgcgctgctg atggcccaac
     2521 ctatcaagag gcgttgagca atgtaaaaat tgtgatccga gaatggctgg aaaccgcaaa
     2581 ggaattggga cgaccgattc cacaaccaaa gggtcgattg gtttttgcct aaactctata
     2641 tcttgaaaag accccaacaa ttcctacagt aaaattcctt ctctcgcttg aggtacattt
     2701 attctaacga cgaagtaaag gattcaaggc taaaccctcc ggcacggggc tgttgctgat
     2761 tcacgagaat atcggtcgat gggcgagaaa gaaggataaa attgtgagat tttcctgatt
     2821 cacagaccca ccagaaatgc ccaatatttc aaaaaagtga aggtgtgggg ggaagatggg
     2881 cgcgaagagg tttcttccat gaaggtttaa ggaacagatg aaaagaaatg ccaatggact
     2941 catcgaaatc atgaagtttt aattaaaaag cgaaggggga ttatttcttt tgccctttgg
     3001 tatttcccca agcatcaaga actgacgcaa tattgtcatg cttcaaactc acagcaaagt
     3061 ccctaagcgt actcccatgt ttattgacaa ggtccggatc tgcccgtttg ggagtaacaa
     3121 tttaacaacg ggtgaacggc cggcaagtgc ggcatcatga agagggatat catctaattt
     3181 tccgcgtgca tttacatctg cctcagtatc taccaagcct tcaatttctt ccaaaattcc
     3241 ccgcgagcag gccacatgaa gtggtgtatt accaaaaccc tttggttaac atcattaagt
     3301 tcaagacaaa caaattcagg taattcttca tttttatagc gtaaaaagtc gctttaagtg
     3361 acaatgaaat acccattacg ggtccttctt attgattagt gacttaacaa ttttggcagt
     3421 gtttcgcaat ccaatctttc agatttttga tcccttgcta taaattatct accacctgtt
     3481 gatgccgtcc cgggtgccct c
//
LOCUS       DATIAL010000155         5484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_780202, whole genome shotgun sequence.
ACCESSION   DATIAL010000155 DATIAL010000000
VERSION     DATIAL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780202"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VLA60_04905"
     CDS             complement(<1..1120)
                     /locus_tag="VLA60_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HSF08729.1"
                     /translation="MRDGVKIAVDVYLPADLKPGHTFPTLLHQTRYWRAIKYRWFVSA
                     FKEDLPRGLIGSYAKQFLQQGYAWVDVDVRGSGASFGNRPIAYSPAEIKDGAEIVDWI
                     IAQPWSNGKVGAMGISYSGGTAEMLLINQHPAVKAIAPMYSGFDLYSEIIFPGGIHLN
                     WFTETWSAITHLLDRNRLPHAGWTANLFVRGVRPVDGDTSLDLLSLALQDHKTNWSPY
                     REASGIVFRDDSPPSRQAANIDALSTQTYADDIAKSGAAIYSYSGWFDGGYQLAAIKR
                     YLHHKKPSHRLMLGPWDHGGKRRISPFALGPAQFDHAAELLKFFDIHLKGMPSGNSND
                     PPIRYYTMGEEQWKTSTRWPPESTPTSMYFQQEGGLSMN"
     gene            2020..2571
                     /locus_tag="VLA60_04910"
     CDS             2020..2571
                     /locus_tag="VLA60_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HSF08730.1"
                     /translation="MASAFGHVVVAYAMGKSLSIPFHTARFWWFTVACCLLPDADVLG
                     LVIGIPYEHMLGHRGLTHSIVFAMLVGLVVPRWAIPTLALGTRPYWVFAVYFFLVTLS
                     HGFLDALTNGGLGIAFFAPFDSTRYFFPLRPIAVSPIGLSAFLSQEGMGVLLSELVWI
                     GVPVGVWLLCLRVIRKTGGKGSQ"
     gene            2660..3583
                     /locus_tag="VLA60_04915"
     CDS             2660..3583
                     /locus_tag="VLA60_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSF08731.1"
                     /translation="MASSSKKVIYAALIGNSLIAVTKFAASAITGSSVMLAEGIHSLV
                     DTGNQGLLLYGLHRAKRPADERYPFGYGKEIYFWSFAVAIMVFALGAGISLYEGVLHI
                     LHPEPIINPMVNYIVLSLSLIFEGVAWYMAFREFSRAKGKWSYLQAVQRGKDPTIFMV
                     LFEDSAAVLGLAVAFIGIWLGQVTGIVYIDGIATCIIGLILAGIAMWLAYETKGLLIG
                     ESANKEVVQSIRDMANQLPGVEHVNEVLTMHMGPNFILVNLSVDFRDGVTSEEVERGV
                     ATLDKDIKVKHPLVKRVFVEAEARRVLKIPE"
     gene            3658..5241
                     /locus_tag="VLA60_04920"
     CDS             3658..5241
                     /locus_tag="VLA60_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08732.1"
                     /translation="MGETRVDLLHLLEDLRDAYPGALEDTILTEIIANALDSGASRIV
                     MDVNPSAGTLMVRDNGSGMQRKDLARYHDIASSTKRRGQGIGFAGVGIKLGLLVCEEV
                     LTETKRGHTHVSTTWHLSSRHRAPWKWVPPIGMVIQQGTAVRLKLHNILSPLLDQAFI
                     EAAISRHFQPLLDPTFSLILNAHYPHGVTFEINGVDLPPQRFEAPLRAPLEIRLARKR
                     KPSAVGYLVRTGNLLPDDLQGLAMSTFGKVIKSGWDWLGITPQAREHISGLIEVPGLA
                     ASLTLNKGDFIRVGARGATYLAYRKAIQEAVQRQLSEWGVVDSPVEETPPRQIRPLER
                     DLERVLEDLSDLFPLLGSLVEHRKGGQKRLLLGTPGPYEAMRAVMVPGEVSQDQETES
                     ELVVQPPGNDSPDGETPREPREPGEDASVVSDFLLPDRGSRRAPARFGLGIQFEDRED
                     DQEIGRLVESTVWVNRAHPAYQRAVATRSVGYHIALAVGLALAPLAVEASSEHAFLTA
                     FLSCWGEAGLPKKSKPPKR"
     gene            complement(5280..>5484)
                     /locus_tag="VLA60_04925"
     CDS             complement(5280..>5484)
                     /locus_tag="VLA60_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08733.1"
                     /translation="IFMTTPEFPSEPQNLIWVVSNYDPGNLSLDTMPSQAVNAFAVSI
                     LWPTCISPIIAWWVLEIEDKDQD"
BASE COUNT         1188 a         1316 c         1642 g         1338 t
ORIGIN      
        1 cattcatgga gagcccgcct tcttgttgga aatacattga cgtgggagtg gactcaggcg
       61 gccagcgagt cgatgttttc cattgttctt cgcccatggt gtagtagcgg atcggtggat
      121 cattcgaatt gccggagggc atgcccttca gatggatatc gaaaaattta agcagttcag
      181 ccgcatggtc aaactgggcg ggtccaagcg cgaaggggct gatccggcgt ttgcctccgt
      241 ggtcccatgg cccgagcatg agccgatggg atggtttttt gtggtggagg taccgtttga
      301 tggcagccag ttgatatccc ccgtcaaacc acccgctgta gctatagatg gctgcacctg
      361 atttggcaat gtcgtcggca taggtctgtg tgctgagggc atcgatattg gcagcctgcc
      421 gcgatggtgg agaatcatcc cgaaaaacaa ttcccgaagc ttcccggtac ggactccaat
      481 tggtcttgtg gtcctgcaga gcgagggaca agaggtcaag gctcgtatcg ccatccactg
      541 gccgcactcc gcgcacgaag agattggcgg tccatccggc gtggggtaat cggttgcgat
      601 caagaagatg agtaatcgca ctccaggttt cggtaaacca gttgaggtgg ataccacctg
      661 gaaagataat ttcagagtag agatcgaagc ctgaatacat cggggcaatc gctttgacgg
      721 ccggatgctg attgatgagt aacatttcag cggtcccgcc actataggaa atacccatgg
      781 ccccgacctt tccattcgac cagggttggg cgatgatcca gtccacaatt tctgcgccgt
      841 ctttgatttc cgcaggggaa taggcgatcg gtctgttccc gaatgacgcg ccggagccac
      901 gcacatccac atcgacccat gcatagccct gttgaaggaa ttgtttggca taggatccga
      961 tgagccctcg gggaaggtct tccttaaagg ccgagacgaa ccatcgatat ttgatcgcgc
     1021 gccaatatcg ggtttggtgt aagagggtgg gaaatgtgtg gcccggctta aggtcggcgg
     1081 ggagatagac atccaccgca atcttgaccc cgtcccgcat cgtgagatac tgcgaggtac
     1141 ggtgaaattt tccgtctggc tctataacgc tgttagctga agaagacgga ctggttgccg
     1201 ccggatcaga atgctcaaca ttatttggcg cacaacctgc gaagataggg agtgccagcc
     1261 aaagcaggag gaaagcccat aagcggaaaa atggggagaa acggctggta gatgggaaac
     1321 ggagattgat ttgtctgaac atatggagtg gatggatatt ggttctggtc ttacagctcg
     1381 acggcttgtc tcggggaccc accttggagt ttaccagtaa aaacgcgtcg tgaggagaag
     1441 gcctcgtatt agtttctcca attctggtgt caatgtgatg ttctatggtg ccgggtttcg
     1501 ccccggcagg cgaggccctt ttgtttcggc aaaagggccc aaaaccattg acgccccgtc
     1561 tggcctcatt agagaggagg gacgccaact aggagggcgg tccaactcgt aaggctcaaa
     1621 caaggctcgc caactgagga gagcgtccct ccattgggcc agacggcagg cgtcggacca
     1681 tgggaggggg ctgttgaaaa aagccgccag cggcgttctc gccatttttc cattctcacg
     1741 tactggaagt acgctctgcg cgcaaaaatg actgcggcct tgctggacga acttttttga
     1801 accgccccga ggtctctaat gcaacctgcc tgggggtcaa tatgaccttg gaaattatta
     1861 ttcaaaactt ccgggaggca aacctttcag tggctcacat gaaagagcga ggagctctct
     1921 acatcagaag atgtcaattg acaaaatgga agcataaagg tttttgcgac atccataata
     1981 atttcatgat aacaagagcg tgaagagaag gagaaggtga tggcctcggc gtttggacat
     2041 gtggtggtgg cgtatgccat gggcaaaagc ctgagcattc catttcatac cgcaaggttt
     2101 tggtggttca cggtggcctg ttgtctcttg ccggatgcgg atgtgctggg attggtgatt
     2161 gggattccgt atgagcatat gttggggcat cgtggcctca cgcattcaat tgtgtttgcc
     2221 atgctggtgg gtttggtggt gccccggtgg gccattccaa ctcttgcgtt ggggaccaga
     2281 ccgtattggg tgtttgctgt gtattttttt ctcgtcaccc tgtctcatgg ctttctggat
     2341 gccctgacca atgggggact cggcattgcc ttttttgcac cattcgactc aaccagatat
     2401 ttctttccct tgaggccgat agccgtttcg ccgattgggc tctcggcctt tttatcccag
     2461 gagggcatgg gagtgctgct cagtgaactg gtgtggattg gggtacctgt tggagtctgg
     2521 ttgttgtgct tacgcgtcat cagaaagacg ggcgggaagg gttcccagtg agcatacaga
     2581 gggcggactt ttatcctggt tatcaggtaa cattgcgtgt tctctcgccg ggaatgtgtt
     2641 tcgaataatt tcaggtctaa tggcgtcatc ttctaaaaaa gttatctacg cagcgttgat
     2701 cggcaatagt cttattgcag tcaccaaatt cgccgcttcg gcgatcaccg gcagttcagt
     2761 gatgctggcc gagggcatcc actctttggt ggataccggc aatcaaggtc tgctgctcta
     2821 tggtcttcac cgcgctaaac gaccggctga tgagcggtat ccattcgggt atgggaagga
     2881 aatctatttt tggagttttg cggtggcgat tatggtcttt gccttgggcg cgggaatttc
     2941 cctctatgag ggcgtgttgc acatcctcca tcctgagccg attatcaacc ccatggtcaa
     3001 ctatatcgtc ttgagtctct ccctcatctt tgagggtgtc gcctggtaca tggctttccg
     3061 ggaattttcc cgtgccaagg gaaaatggtc atatttgcaa gcggttcagc ggggaaagga
     3121 tccgaccatt tttatggtcc tctttgagga ttcggcggcg gtgctgggct tggccgtggc
     3181 ctttattggc atctggttgg gacaggtgac cgggattgtc tatattgacg gcattgccac
     3241 ctgtattatc ggattgattt tggccgggat cgccatgtgg cttgcgtacg aaaccaaggg
     3301 attgttgatt ggtgaaagcg ccaataagga agtggtccag agcattcgtg acatggccaa
     3361 tcagcttccc ggcgtggagc atgtgaatga agtgttaacc atgcacatgg gaccgaattt
     3421 tattttggtg aatctgagcg tggactttcg agacggggtc acgtcggagg aggtggaacg
     3481 cggcgtggcc acattagata aggatattaa ggtgaagcat cccctcgtga aacgggtgtt
     3541 tgtggaggcc gaagcccgac gggtcctcaa gattccggaa taatgtgtat aaaggtcctt
     3601 gtgtcaatta ggtgcaaagg ggcgcttaag acctaaaaaa atggatacac gatcgcgatg
     3661 ggtgaaaccc gggttgattt gcttcatttg cttgaagatt tgcgggatgc ctatccgggc
     3721 gcgctcgaag acaccattct taccgaaatc attgcgaacg ctctggattc aggggccagc
     3781 cggattgtga tggacgtgaa tccgtccgcg ggaacgttga tggtgcgaga taacgggtcg
     3841 ggcatgcaac ggaaagactt agcccgctat catgacattg catccagcac taaacgacgg
     3901 ggacagggga ttggctttgc cggtgtgggt attaagctgg ggttgttggt atgtgaggaa
     3961 gtcctgacgg aaaccaagcg aggacacacg catgtgtcta ccacctggca tttgtcgtca
     4021 cggcatcggg ctccctggaa atgggttccc ccgatcggca tggtgattca acaaggcact
     4081 gctgttcgtc tgaaactcca caatattctc tctccactcc tggatcaggc atttattgag
     4141 gcggccatct ccagacattt ccaaccgttg ctggatccca cgttttccct catcctgaat
     4201 gctcactatc cccacggggt gacttttgaa atcaatgggg tggatctgcc gcctcaacgc
     4261 tttgaggcgc cgctgcgtgc gcctttggaa attcgcttgg cgcgcaaacg gaaaccctct
     4321 gcggtgggat atctggtgcg aacgggaaat ctcttgcccg acgatcttca aggattggcc
     4381 atgagcacct ttggcaaagt catcaagtcc gggtgggatt ggttggggat cactccgcag
     4441 gcacgggaac acatttccgg actgatcgag gtgcctggtc tggcggccag cctcacattg
     4501 aataagggtg atttcattcg tgtgggagca cgaggcgcga catatttggc ctatcggaaa
     4561 gccatccagg aagccgttca acgtcaactt tcagaatggg gcgtggtgga tagtccggtc
     4621 gaggaaacgc caccccgcca aatcagaccg ctggaacggg atcttgagcg cgtgttggaa
     4681 gatctctccg acctctttcc gctcctgggc tcgttggtcg aacatcggaa gggcgggcag
     4741 aagcggttac tgttgggaac gccgggcccc tacgaggcca tgcgggcggt gatggttccc
     4801 ggagaagtct ctcaggatca ggaaacggaa tcagaactcg tcgttcaacc tcccggcaat
     4861 gattctccag atggggagac cccacgtgag cctcgcgagc caggagaaga tgcctcggtg
     4921 gtgtctgact ttctgctgcc ggaccggggc agccgaaggg ctccagcccg gtttgggctc
     4981 ggtattcaat ttgaagaccg ggaagacgat caggaaatcg ggcggttagt ggagtcaacg
     5041 gtgtgggtga atcgtgccca tcctgcctat caacgggcgg tggcgacgcg gtcggtcggc
     5101 tatcatatcg ccctggcggt ggggttggcc ttggcaccgc tagccgtaga ggcttcaagc
     5161 gagcatgcct ttctcacggc ctttctctcc tgttggggcg aggccggtct tcccaagaaa
     5221 tcgaagccac ccaagcggta aagcctggct ggttcgcgaa tggctgaacg gagggtttcc
     5281 taatcctggt ccttatcctc gatttccaat acccaccagg caatgattgg ggagatgcag
     5341 gtaggccaga ggatactcac cgcgaatgcg ttgacagcct gcgacggcat agtatcgagg
     5401 ctcaaattgc ccggatcata gtttgagaca acccaaatta agttctgtgg ttctgatgga
     5461 aactccggtg tcgtcataaa aatg
//
LOCUS       DATIAL010000156         5441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_350780, whole genome shotgun sequence.
ACCESSION   DATIAL010000156 DATIAL010000000
VERSION     DATIAL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350780"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VLA60_04930"
     CDS             complement(<1..888)
                     /locus_tag="VLA60_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSF08734.1"
                     /translation="MTVTPAIGQSHDTPQDNSIRERMNIVIVGHVDHGKSTLLGRLYA
                     DTGTLPTGKIEKIQAICKQQGKEFEYAFLFDAFLEEQQQGITIDTARTFFSWGGRQYI
                     IIDAPGHKEFLKNMVSGAARAEAALLVIDALEGVRDQSKRHGLLLSMLGVKQVTVVVN
                     KMDLVNYREDTFHAIEKEYREFLTQLNVIPQYVIPASAKQGINIIKPSPETPWYTGPS
                     VLDSLAHFHLESKRAEQTLRLPIQDVYKFDARRILVGRITAGKLKIGDRLVFSPSNKS
                     ANIETIEAFNIDPPPTQAEA"
     gene            complement(891..1691)
                     /gene="cysD"
                     /locus_tag="VLA60_04935"
     CDS             complement(891..1691)
                     /gene="cysD"
                     /locus_tag="VLA60_04935"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HSF08735.1"
                     /translation="MKHIRQLEDQSVYILREAYKNFDNLAMLWSMGKDSTVLLWLARK
                     AFFGHVPFPLLHVDTSYKIPEMIEYRDRLAREWRLNLIVGQNKKALAEGMNHTKGRVE
                     CCTALKTQGLKQLLEEKGYTGVILGVRSDEDSTRAKERYFSPRDKNNEWDFRDQPPEL
                     WDQYKLSFPPGTHIRIHPLLDWTEINIWEYIKLENIPFLDNLYLDQGTGKRYRSLGCA
                     PCTTPIDSTAKNVDEVIIELRNTKVAERAGRAQDEGRGMEQLRKDGYM"
     gene            complement(1688..2776)
                     /locus_tag="VLA60_04940"
     CDS             complement(1688..2776)
                     /locus_tag="VLA60_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08736.1"
                     /translation="MDHLIAKIGKGLKGAKDLTWDEAKLALNAIIEGQATDVQVGAFL
                     MAMRIKTESISELAAFTTTVRRWAPPIPLPHAENIVDLPLYGEKHNTIHVVIAASLVA
                     ASAGAGLFMHGVENPDLPYDLPQVLRHLQLPIANTAAEAIEHGAQSPWVYMDLAGYHA
                     PLTKLLDLRQAFGLQNLSHQVSRLLNPARVGSQVIGISHPPYLDKMVEALDMLGAKRA
                     LIIQGVEGFPELSLSAPSLARELRSGHISSLVFRPDDAGFRSGPFQAMSSTINGPGSG
                     NPQHEAQLITQLLIDRQKGDRRNWVIMNAALLMYAAGLASSLSQATPLAQDTLDSGKA
                     GAFFTALAQGHPPQSKFVVPPAPAVVPA"
     gene            complement(2791..3204)
                     /locus_tag="VLA60_04945"
     CDS             complement(2791..3204)
                     /locus_tag="VLA60_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSF08737.1"
                     /translation="MKKIAVIITRGGWNNLFQACEWIALAAASGIEVSGYFRDEAAGR
                     MTKTKIKELTMSTEFKGREGFVRELLKKEDKADLSKLMQTSKEKGNVKFSVCKDSLKY
                     FGVKVEELIPELDEVQTAEAFWKEAVLPADQVLTF"
     gene            complement(3284..4000)
                     /locus_tag="VLA60_04950"
     CDS             complement(3284..4000)
                     /locus_tag="VLA60_04950"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HSF08738.1"
                     /translation="MALFLNTHPTPEELIAVNRSMEGQTPRKIVETAIAEYGGTIILA
                     CSFGAEDVVLADMMFRANPGSTLFYLDTDFLFPETHAVRNRMIQHYQLRDDQIIQVKS
                     MLSPSKQATEFGEALWLRDPDQCCSLRKVEPLTRILGKYAAWMTGIRRDQSPTRAKAA
                     IVEWDAKFGLVKFNPLAAWSWEQVWEYIRTNAVPYNTLHDQHYPSIGCTHCTAPVMPG
                     DDPRSGRWKSSGKTECGLHR"
     gene            complement(4007..>5441)
                     /locus_tag="VLA60_04955"
     CDS             complement(4007..>5441)
                     /locus_tag="VLA60_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSF08739.1"
                     /translation="AVVRLPSGDITSEQMFGLADLAERYANGNLRTTINQNIMVRWLP
                     ELRLRHFYDELVAHGLGDPGAEGVADIVSCPGTDTCGLGITSSKGLARALAEVFPAGK
                     IAEDLEGVNVKISGCHNSCAQHHIATIGLHGVGKRIGDHVAPVYELHLGGRVNGTAKI
                     AQLIVKVPAKNVPAAVQHLLDLYRRDRKNGESLLAFIDRTGKLQLKDELIPYTILPTY
                     QEDPQFYIDWEGDEEFSVEDLGPGECAGGALEMIDNRILEAEQELYQARLLAEKHQYA
                     FAINKAYRAVVAGAKAILVTEGIDPNTDADTLAEFDKLIVAKGLMPAEYHNLAMTVGD
                     LGNKDASADLTQGKIAFTKGFVDLCRTVSDQIGQDLKLAPAELGQKSTESTRTVPTPP
                     DKATSPSGVPVYDLRGVACPMNYVKTKLKLEMMDHGDQLEVWLDGGDPIRNVPVSLRN
                     DGHNVLKQEPIDAEEQHFRVLVEKVEL"
BASE COUNT         1188 a         1384 c         1427 g         1442 t
ORIGIN      
        1 ggcttcggct tgagttggcg gtggatcgat attaaaagct tcaatcgttt cgatattggc
       61 tgacttgttg gagggggaaa agaccagccg gtcaccaatc ttgagttttc ctgccgtgat
      121 ccggccaact aaaatccggc gggcgtcgaa cttatacacg tcctggatgg gaagacgaag
      181 cgtttgttcg gcacgcttgg attccagatg gaaatgcgcc agggaatcca atacggatgg
      241 tccggtgtac cacggggttt ccgggctcgg tttgatgatg ttgatcccct gtttggcgct
      301 cgcggggata acgtattggg gaataacgtt caattgggtg agaaattccc ggtattcttt
      361 ttcgatggcg tggaaggtgt cttcccggta attgaccagg tccattttat tgacgaccac
      421 cgtcacctgt ttaactccta acatggagag caacagccca tgccgttttg attggtcgcg
      481 tacgccttcc agggcatcaa tgaccaaaag ggccgcttcg gcacgagcgg cacctgacac
      541 catgtttttc aagaattcct tatggcctgg tgcgtcaata atgatgtatt gtcgcccgcc
      601 ccaggagaaa aaggttcggg ctgtgtcgat ggtgatcccc tgttgttgct cttctaaaaa
      661 cgcatcaaaa agaaatgcgt attcgaattc tttgccctgt tgcttgcaaa tggcctggat
      721 tttttcgatc ttgccggttg gtaaggttcc ggtgtcggca tacagacgac ccaatagcgt
      781 ggatttgccg tggtcgacgt gacccacgat cacgatattc attctctccc ggatggaatt
      841 gtcctgggga gtgtcgtggg attggcctat ggctggtgtg acggtcatct tcacatgtat
      901 ccatctttgc gtaattgttc catgcctcgt ccttcgtctt gtgcgcgtcc tgcccgctcc
      961 gccaccttgg tgttgcgtaa ttcaatgatg acctcatcta catttttcgc ggtggaatca
     1021 attggtgtcg tgcatggggc gcatcccaga ctgcgatagc gtttgccggt gccttgatcc
     1081 aggtagagat tatccagaaa gggaatattt tctaatttga tgtattccca aatattgatc
     1141 tccgtccaat ccaaaagggg atggatccgg atatgggtgc ccggaggaaa cgaaagttta
     1201 tattgatccc aaagttcggg gggctgatcg cgaaaatccc attcattatt tttgtcccgc
     1261 ggggagaaat accgctcctt cgccctggta ctatcctcgt ccgatcgcac acccaaaatg
     1321 acccctgtgt agcccttttc ttccagcaat tgttttaacc cttgggtttt taaggccgtg
     1381 caacactcca ctcgtccctt cgtgtggttc atcccttcgg ccagggcttt tttgttttgg
     1441 cccacgatca gatttaatcg ccattcgcgt gcgaggcgat cccggtattc aatcatttca
     1501 ggaatcttat agctggtgtc tacatggagt aacgggaagg ggacatggcc aaaaaatgct
     1561 tttctggcca gccacaataa cactgtagaa tctttgccca tggaccacag catggccagg
     1621 ttgtcaaagt ttttgtaggc ctcacgcagg atatagacgc tttgatcttc taactggcgg
     1681 atatgtttca tgcgggcacc accgccggtg caggtgggac aacgaatttg gactggggag
     1741 ggtgtccttg ggccaaggcg gtaaagaacg ctccggcttt tccggaatcc agggtatcct
     1801 gggcaagtgg tgtggcttgc gaaagagagg aagccaaccc tgcggcatac atcagtaagg
     1861 ctgcattcat gatcacccag ttccgtcggt cgcctttctg gcgatcgata agtaattgcg
     1921 ttatcaattg ggcttcatgt tgggggttcc cggatcccgg cccattgatg gtcgaactca
     1981 tggcttgaaa tggtccggat cggaatccgg catcatcggg acgaaagact aacgatgaaa
     2041 tatgcccgct tcgtaattct cgcgccaagg agggggcgga gagggacagt tcgggaaatc
     2101 cttcgactcc ttgaataatc aatgcccgct tcgcgccaag catgtccaaa gcttccacca
     2161 ttttatccag gtatggagga tgggaaatcc caatgacttg tgagccgacc ctggccgggt
     2221 tcaggagtct ggacacctga tgggacagat tttgcagtcc gaatgcctga cgcaggtcca
     2281 gtaatttggt taacggtgca tgatatcctg ctaaatccat gtacacccag ggcgattgag
     2341 cgccatgttc aattgcttca gccgcagtat tggcgatagg aagctgtaag tgacgaagga
     2401 cttgagggag atcgtagggg aggtcggggt tttctacgcc atgcatgaag agacctgcgc
     2461 cggctgaagc cgcgacaaga cttgcggcga tgaccacatg gatggtgttg tgtttttctc
     2521 catagagagg caggtccaca atgttttcgg catgcgggag tggaatcggt ggcgcccacc
     2581 tgcggaccgt tgtggtgaac gcggcgagtt cgctgatgga ttcggttttg atacgcatgg
     2641 ccatcaaaaa cgctcccacc tgaacgtcag tggcttgccc ttcaataatg gcgttcagag
     2701 ccagcttggc ctcgtcccag gtcaaatcct tggcaccctt cagccctttt ccaatttttg
     2761 caatcagatg atccatgcca tgattccggg ttaaaaggtc aaaacctggt cagcgggaag
     2821 gacagcttct ttccagaagg cctcggcggt ttgcacttca tctaattccg gaataagttc
     2881 ttcgactttc actccaaaat atttcagaga atccttacac accgaaaatt tgacgtttcc
     2941 tttttcttta gaggtttgca tgagtttcga gaggtccgct ttatcctctt ttttcaagag
     3001 ttcacgaaca aagccttctc gccctttaaa ctccgtggac atggtcaatt ctttgatctt
     3061 ggttttggtc attcgacctg cggcttcgtc acggaaatag ccactcacct cgatgccact
     3121 ggccgctgcc agggctatcc actcacaggc ctggaacagg ttgttccatc cccctctggt
     3181 aataatgacg gcaatttttt tcactggcaa tctccaatag ctacttgatt gagaaaaatg
     3241 aatgtaaagt catgaatgac ggcatggtta cgtggctgga ttatcatcga tgaagtccac
     3301 attcggtttt ccccgaactt ttccatcgtc cggagcgtgg gtcgtctcct ggcataaccg
     3361 gtgccgtgca atgggtacat ccaatgctgg gatagtgttg atcatgcaat gtattatagg
     3421 gaacagcatt cgtgcgaatg tattcccaga cctgttccca tgaccaggcc gctagggggt
     3481 taaattttac aaggccaaac tttgcatccc actcgacaat ggcggctttc gcgcgagtgg
     3541 gtgattgatc acgccgaata cctgtcatcc acgccgcata ctttcccagg atgcgcgtga
     3601 gaggctcaac ctttcgcagt gagcaacatt ggtcgggatc ccgcagccat aaggcttccc
     3661 caaactctgt ggcttgtttg gaaggactga gcattgattt gacctggatg atctgatcat
     3721 cccttaactg atagtgttga atcatccgat tccgaacggc atgggtttca ggaaataaaa
     3781 aatccgtatc taaataaaag agtgtgctgc ctggattggc acgaaacatc atatcggcca
     3841 gaacaacatc ctcggcccca aaactacagg ccagaataat cgttccccca tattccgcaa
     3901 tcgcggtctc gacaatcttc cgaggagtct ggccctccat tgatcgattg accgcgatga
     3961 gttcttccgg agttggatgg gtattgagga aaagagccat ggcgttttac aattctactt
     4021 tctccactaa cactcggaaa tgctgctcct cggcgtctat gggttcctgt ttgagcacat
     4081 tgtgcccatc gtttcgaaga ctcactggga cgttgcgaat cggatcgccg ccgtccaacc
     4141 atacttccaa ctggtcaccg tggtccatca tttctaattt caacttggtt ttcacgtaat
     4201 tcatgggaca ggctacgccc cgcaggtcgt agacgggcac gcctgacggg gaggttgcct
     4261 tgtcaggtgg cgtgggtacc gtcctggttg attcagtgga tttttgtccc agttcagcag
     4321 gagccaactt gaggtcctgg ccgatttggt ccgaaacggt ccggcagaga tccacgaaac
     4381 cttttgtgaa tgcgattttt ccttgcgtca ggtcagccga ggcgtctttg ttccccagat
     4441 ctcccactgt catggccaga ttgtggtatt ccgccggcat aagccctttg gccacaatca
     4501 acttgtcaaa ttccgctaag gtgtcggcat ccgtattcgg atcaatcccc tctgtgacaa
     4561 ggatggcttt tgctcctgcc accacggctc gataggcttt gttaatggca aacgcgtatt
     4621 ggtgcttttc agccaacagg cgtgcttgat acagttcctg ttccgcttca aggatccgat
     4681 tgtcgatcat ctccaatgcg cccccggcgc attcgccggg tccgaggtcc tcaaccgaaa
     4741 attcctcatc gccttcccag tcaatataga attgtggatc ctcttgatat gtgggaagaa
     4801 tggtgtaggg gatcagttca tcttttagtt ggagttttcc agtgcgatca atgaacgcca
     4861 ggagggactc cccatttttt ctatcccggc gatagagatc cagcagatgc tggacggcag
     4921 cggggacgtt tttcgcaggg accttcacaa tgagttgggc aattttggcc gtgccgttga
     4981 cccgtccccc aaggtgcaat tcatacactg gcgccacatg gtctccaatg cgtttgccga
     5041 ctccatgcaa gccgattgtg gcgatatgat gttgagcgca ggaattgtgg cagccactga
     5101 ttttcacgtt caccccttcc aggtcctctg cgatctttcc ggcagggaac acttctgcca
     5161 gcgctcgagc cagtccctta gaagaggtga ttcccagtcc gcaggtgtcg gtccctgggc
     5221 aggagacgat atcggcgacg ccttcagctc cagggtcgcc caacccatga gcgactaatt
     5281 cgtcatagaa gtgccggaga cgcagttcgg gaagccatcg caccatgatg ttctgattga
     5341 tggtggtacg aaggtttccg ttggcatatc gttcggcgag atcggccagt ccaaacattt
     5401 gctcagacgt aatatcaccg gaggggagtc tcaccaccgc c
//
LOCUS       DATIAL010000157         5599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_995108, whole genome shotgun sequence.
ACCESSION   DATIAL010000157 DATIAL010000000
VERSION     DATIAL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995108"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..643
                     /locus_tag="VLA60_04960"
     CDS             212..643
                     /locus_tag="VLA60_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF08740.1"
                     /translation="MLCDSVGKYILMGLIKMGGKGQRGFGKTLAVVLMAAFSIVEPGH
                     TNTAQEIDRPNPSSPFLERIRQGKSLYEGLARCVHCHGQTALRRPLTRQELFSIIKFG
                     VPGTSHMPFTYLLSDEQIWTIVEYQLREACENGCVREPSSE"
     gene            complement(854..1168)
                     /locus_tag="VLA60_04965"
     CDS             complement(854..1168)
                     /locus_tag="VLA60_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08741.1"
                     /translation="MSHPEYMTMTEALKGLRSRGFTTNFAFLHHAFCAVESGKTFKPE
                     ELSIREHHRFEGISDPDDESVVYAVETHDGLRGTIVDAYGIYANSELEAFLEKVDMQE
                     ER"
     gene            complement(1261..3684)
                     /gene="ppsA"
                     /locus_tag="VLA60_04970"
     CDS             complement(1261..3684)
                     /gene="ppsA"
                     /locus_tag="VLA60_04970"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HSF08742.1"
                     /translation="MKPPASSPESYILWFEEIGLQDISRVGGKNASLGEMYRELTPQG
                     VRIPNGFAITAEAYRYVLREAGLDTKIQQILGDLDTGDMSNLRQRGRQIRQAIIGATL
                     PPALEQAIEEAYARLSAQSKDGADVAVRSSATAEDLPDASFAGQQETYLNVQGHQALF
                     ETCKRCFASLFTDRAISYRVDKGFDHLQIALSIGVQQMVRSDLASAGVLFTIDTETGF
                     PDVVLINASYGLGENVVQGAVNPDEYYVFKPTLKQGFQPILHKITGSKEFKLIYDIGG
                     NKMVKNVPVSPDDRNRFAMNDEEILTLARWGCLIEDHYSGKKGRACPMDIEWAKDGQT
                     GELFIVQARPETVQSRKDPDVMETYHLTKRGQVLIQGRSVGEKIGQGPVRVITQVQNL
                     QDFREGDVLVTDKTDPDWEPIMKKASAIVTNRGGRTCHAAIVSRELGLPAIVGAERAT
                     DALKNEQSVTVSCAEGDTGFVYEGRLPFDVERVSLKNLGHPRTKVMMNIGNPEEAFSL
                     SGIPNAGVGLARLEFIISTFIKIHPLALLEYGQIMDAGVKADIDRLTSAYPDKTQYFV
                     DKLAQGVGMIGAAFYPKDVIVRMSDFKSNEYANLIGGREYEPTEENPMLGFRGASRYY
                     DPRYREGFALECRAMKKVREEMGLTNVKLMIPFCRTVEEGKKVLEEMAKHGLKRGDKG
                     LEVYVMCEIPSNVILAEEFAGIFDGFSIGSNDLTQLVLGVDRDSEIVAHVFDERNPAV
                     KTFIAQVIKAAKTKGRKIGICGQAPSDYPEFAQFLVEQGIDSISLNPDAILKTTVKIL
                     EMEHSLDKN"
     gene            complement(3737..4120)
                     /locus_tag="VLA60_04975"
     CDS             complement(3737..4120)
                     /locus_tag="VLA60_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08743.1"
                     /translation="MDHAKREQSLLQVLEAFTKGLTTLKEAIALVHSANDQLPAGEPR
                     KAIQQKIEEAERAIQVGHAAMGQSLGFQLCHCTWPPQVMASSHYSSETKVEQFTCPNC
                     EKTLSLSGRQAPDTAMTAFDPYDVY"
     gene            complement(4195..>5599)
                     /locus_tag="VLA60_04980"
     CDS             complement(4195..>5599)
                     /locus_tag="VLA60_04980"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HSF08744.1"
                     /translation="SAYEWHIPQKGTMRVPGIIYADESLIRDMDLKVYEQVVNVATLP
                     GIVQASFAMPDAHWGYGFPIGGVAAFDPQQGGVVSAGGVGFDISCGVRALYTGLRTDD
                     ITPVKEKLADLLFAQVPAGVGSTGRLHLKPKEMDDMLAGGAQWAVQRGYGTVKDLSHI
                     EEHGCMTGAKPAYVSDQAKKRQQDEMGTLGSGNHYLEIQEVVETYHDPLSEKLGIHSG
                     DILVSIHCGSRGLGHQIGTEYLKKMVVAASQYGITLPDRELACAPIDSPLGQEYLGAM
                     RAAINCALANREIVTHLVRQAFSEIFPQTSLPLLYDVSHNTCKMEEHQIGGRSRRVFV
                     HRKGATRAFGPGHAAIPADLQAFGQPVLIGGTMGTASYLLVGTKEGMGLAYGSACHGA
                     GRSMSRHQATRHWKGQRVIQELGAQGILIRSPSARGVAEEAPGAYKDVTAVVDAAEQA
                     HLARKVAKMRPLICIKG"
BASE COUNT         1241 a         1498 c         1443 g         1417 t
ORIGIN      
        1 taactagtta ctaaagtaaa gtaggtacta taaagtaacc aatgaattcc tctgaaagtc
       61 ctattaatgt caactggacc gagggcataa gaacgggtag gttggtttaa aaatttgtcg
      121 gatcgatcag gtgaatgcgc tgatctctgg agggcaggtt ggttgaccag gatggcaaac
      181 tccacccttc gacgcctgta tgacaaactt catgttgtgt gattcagttg ggaaatatat
      241 tctgatggga ttgatcaaaa tgggcgggaa gggtcaaaga ggttttggga aaaccctggc
      301 tgttgtgttg atggcggctt tttctatcgt tgaacccggc cataccaata cagctcagga
      361 aattgacaga ccgaacccct cgtctccctt cttagagcgg attcgacagg gtaaaagctt
      421 gtatgagggg cttgccaggt gcgttcattg tcatggtcaa accgccttgc gacgtcctct
      481 tacccggcaa gaactttttt ccattattaa attcggcgtg ccggggacat ctcatatgcc
      541 ttttacatat ctgctttcag acgagcaaat atggaccatc gtggagtacc agcttcgcga
      601 ggcatgcgaa aacggttgcg tcagggaacc atcttcagaa taaagcactc aggttatttt
      661 caggttttga taaaatgggc acaggactga gagtggatac tgcctccctc aatgcgtggt
      721 atccggttgc ggaaagggat atgtcgatca tggttcccgg gtcgacacct ccagtgagtg
      781 tcaaaaaata ttttggggct cgtgaatacc ggagggtcgg gcgaaatggc cggtattgcg
      841 accgcaatgt ctttcaccgt tcttcttgca tgtcgacctt ttccaaaaac gcctcaagtt
      901 cggaattggc ataaatgcca taggcatcga cgatagttcc tcgcagacca tcatgcgttt
      961 caacggcata caccaccgac tcatcatcgg gatcactgat gccttcaaac cgatgatgct
     1021 cacggattga gagttcttcc ggtttgaacg ttttcccgct ttcgacagca caaaaggcat
     1081 gatgaagaaa ggcaaagttg gtggtaaagc cccggctcct tagtcccttt aacgcttcgg
     1141 tcattgtcat atattcggga tgtgacatgg cgtctatggc tcctttctct tttagtgggt
     1201 acgtggggga gtgatcgccc cgtccttggg caaccatttc gaaggaggcg ccttaggaga
     1261 tcaattctta tctaaagaat gttccatttc aagaattttg accgtggttt tgaggatagc
     1321 atcgggattc aaggagatgc tgtcgatgcc ctgctccaca aggaactgcg caaactcggg
     1381 atagtcactg ggggcctggc cgcagatgcc gatcttccga cctttggtct ttgcggcctt
     1441 tatgacctgg gcgatgaatg ttttgactgc gggattgcgc tcatcaaaca catgggccac
     1501 gatttctgaa tcccggtcca cgccgagcac cagttgtgtc agatcatttg agccgatgga
     1561 aaatccatcg aagatcccgg caaattcttc cgccagaata acgttactcg gaatttcgca
     1621 catcacatag acttccaggc ccttgtcgcc acgcttcagt ccgtgtttgg ccatctcctc
     1681 aagaaccttt ttgccttcct ccactgtccg gcaaaatgga atcataagtt tcacgttggt
     1741 aagccccatc tcctccctga cttttttcat ggcccgacat tctagggcga atccctcccg
     1801 gtagcgggga tcgtagtagc gggatgcgcc acgaaatccg agcatgggat tctcttcggt
     1861 cggttcatac tcccggccgc cgattaagtt tgcatattca ttggatttga agtcgctcat
     1921 ccggactatg acatcttttg gataaaacgc cgccccgatc atgccgaccc cttgggcgag
     1981 tttgtcgacg aagtactgtg ttttgtcggg ataggcggac gtgagacgat ctatatcagc
     2041 tttcacgccg gcatccatta tttggccata ctccagcaat gccaagggat ggattttaat
     2101 gaaggtgctg ataataaatt ctagacgagc caacccgacg ccggcattgg gaataccgga
     2161 aagcgaaaag gcttcctcag gatttccgat attcatcatg actttggtgc gggggtgacc
     2221 caaatttttc agagaaaccc gttcgacatc aaacgggagc cggccttcat agacgaagcc
     2281 ggtgtcacct tccgcacaag acaccgtcac cgattgctcg ttcttgaggg cgtcggtcgc
     2341 ccgctctgca ccgacgatag cgggaagacc gagctcccga ctgacgatgg ccgcatggca
     2401 ggttcgtccc cctcgattgg tcacgatggc cgacgctttt ttcatgatcg gttcccagtc
     2461 cggatcggtt ttgtcggtaa ccaacacgtc tccttctcga aagtcctgaa ggttctggac
     2521 ttgggtgatc acccgaaccg gtccctgacc gattttctct cccacgctcc ggccttgaat
     2581 gagaacctgt ccccgcttgg tcaggtgata tgtttccatc acatcgggat cttttcggga
     2641 ctgaaccgtt tcgggccggg cttgaacaat aaacagctca cccgtctgtc cgtccttggc
     2701 ccattcgatg tccatgggac aggcccggcc ttttttcccg ctgtaatggt cttcaatgag
     2761 gcaaccccat cgggccaacg tcagaatttc ctcatcgttc atggcaaaac ggtttcggtc
     2821 atcaggggag accggcacat ttttgaccat cttgtttccc ccgatatcgt aaatcagttt
     2881 gaattccttg cttcccgtaa ttttatgaag aatcggctga aatccctgct tgagggtggg
     2941 cttaaagaca taatactcat caggattaac ggcgccttgc acgacatttt cccccagccc
     3001 ataggaagcg ttgatcagaa cgacatcagg gaatcccgtt tccgtatcaa tcgtgaagag
     3061 cacacctgct gaggccagat cggaacgcac catttgttgc actccgatag acagcgcgat
     3121 ttggagatgg tcgaaccctt tatcgactcg ataggaaatg gcccgatcgg tgaacaacga
     3181 cgcaaagcat cgtttgcagg tttcaaagag ggcctgatgg ccctgcacat tgagataggt
     3241 ttcttgttgt ccggcaaaac tggcatccgg taaatcctcc gctgtcgcac tgcttcgtac
     3301 ggccacgtcg gctccatcct tcgactgggc actcagtctg gcataggctt cctctatggc
     3361 ctgttccaat gctggaggaa gggtggcacc aataatcgcc tgtcggattt gtcgccctcg
     3421 ttgacggagg ttggacatat cgccggtatc caggtcgccc agaatctgtt ggattttggt
     3481 gtcgagtccg gcctcacgca gcacataccg ataggcctca gccgtgatgg caaaaccgtt
     3541 gggaatcctg accccctgcg gagtgagctc ccggtacatt tcgccaagag aggcattttt
     3601 gcctcccact cgactgatat cctgtaggcc aatttcttca aaccagagga tgtaggattc
     3661 aggtgatgag gcaggaggtt tcataccaat gacgttcctt ttttatattt caaaaccagt
     3721 aaatcggcat ggtccgttaa tacacatcgt aagggtcaaa ggcggtcatg gctgtatccg
     3781 gtgcctgccg acctgagaga gaaagtgttt tttcacaatt gggacaggtg aattgttcaa
     3841 ccttcgtttc gcttgaatag tggctgctgg ccatcacctg agggggccat gtgcagtgac
     3901 agagttgaaa gcccagggat tgccccatgg ccgcatgacc aacctggatc gcccgttccg
     3961 cttcttcaat cttttgttga atggcttttc gtggttcgcc tgccggcagt tgatcgttcg
     4021 cggaatggac gagggcaatc gcctccttta aggtcgtcaa ccctttcgtg aaggcttcca
     4081 atacctggag aagactctgt tctcgttttg cgtgatccat aacaaattcc ccttgtgtta
     4141 atgaatgatg ccaatgcgac gtcctggttg tagtcgtgtg agatggtcga gctcttatcc
     4201 cttaatacag attaacgggc gcattttcgc taccttccgg gccaagtgcg cctgttcagc
     4261 cgcatcgacc acggcggtga cgtctttata ggcgcccggc gcttcttcgg cgaccccccg
     4321 tgcggatgga cttctgatca ggattccctg agcccctaat tcttgaatca ccctctgacc
     4381 tttccaatgg cgggtggctt gatgccgact catactgcgt cctgctccat gacaggcgga
     4441 cccgtaggcc agacccatgc cttctttcgt tccgaccaga agataggagg cggttcccat
     4501 ggttccaccg ataaggaccg gttgaccgaa ggcttggagg tcagcaggaa tggccgcatg
     4561 tcccggacca aacgcacggg tggcaccttt tcgatggaca aagacgcgcc gactccggcc
     4621 gccgatctga tgctcctcca ttttgcaggt gttgtgtgac acatcgtaga gcagcggaag
     4681 ggacgtctga ggaaagatct ccgagaaggc ttgacgcact aaatgcgtga caatttcacg
     4741 attggctaag gcgcaattga ttgccgctcg catcgcccct aaatattcct ggcccaaggg
     4801 ggagtcgatc ggggcacagg ccaattcgcg gtccggcaac gtaatgccat actggctggc
     4861 cgccaccacc attttcttga ggtattcggt gccgatttga tgcccgagcc ctctggatcc
     4921 gcaatgaatg ctgaccagga tatccccgga atggataccg agtttttccg acaagggatc
     4981 gtgataggtt tccacgacct cctgaatttc caaataatga ttccctgatc ctaacgtgcc
     5041 catttcgtcc tgctggcgct tcttggcctg atcagatacg tacgcgggtt ttgccccggt
     5101 catgcagccg tgctcttcaa tgtgcgataa atctttcacg gtcccatagc ctcgttggac
     5161 cgcccattgg gcgcctccgg cgagcatgtc atccatttcc ttgggtttca ggtgcaatcg
     5221 accggtactc cccacaccag cgggcacctg tgcaaataag aggtccgcca gtttttcttt
     5281 cacgggcgtg atatcgtccg tccgtaagcc cgtatagagg gcccgtacgc cgcaggaaat
     5341 atcaaacccc acaccgccgg cagacaccac cccgccctgt tgagggtcga aggccgcgac
     5401 tccgcctatc ggaaatccat agccccagtg agcatcgggc atggcaaacg aggcttggac
     5461 aattcccggg agtgtggcca cattgacgac ctgttcatac accttcagat ccatgtcgcg
     5521 aatgagtgac tcatccgcat aaataattcc cggcacgcgc atcgttccct tctggggaat
     5581 gtgccactca tacgcagag
//
LOCUS       DATIAL010000158         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_672607, whole genome shotgun sequence.
ACCESSION   DATIAL010000158 DATIAL010000000
VERSION     DATIAL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672607"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VLA60_04985"
     CDS             complement(<1..336)
                     /locus_tag="VLA60_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08745.1"
                     /translation="MVAAFLLGGVPVGAEQCFEDQYRYLVQLPYEPPSQPEYTPGEKA
                     TPAPEVLTLQDQERLESLIPLLEGRQEFWAIGEFVHYGRHSVPYLVKALKMPGPRVRF
                     NAVETLSMIK"
     gene            complement(468..1505)
                     /locus_tag="VLA60_04990"
     CDS             complement(468..1505)
                     /locus_tag="VLA60_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSF08746.1"
                     /translation="MMFLTLALLGGCSESSTSSEPDGSRRPTLYYFTWSDYVAPDIVA
                     KFETSRGVRVVVDTFSSNEELLAKVQTGMTGYDVIVPSDFMAGLMGRLGLLADLDLNK
                     IPNVQHLEPHLQNLPFDPTHRFAIPYLWGTVGIGYNAEVVTKPPTSWEALWDPQFSGR
                     ISMLNDEREVFGLALRALGYSLNSVDPQAIQQAKQKLIVQKPLVKAYTSEQFDQLLVA
                     GEVVLAHAWGGPVARAMRERPSIRYAIPREGGTIWTDCLAVLASSPNQALAMDFINFL
                     LDEEVALLTSQRLLFASANRHVRNRLTADIRDNHAVYPPDEILPRMEWMEDVAEAIRY
                     YDRAWTELKVQ"
     gene            complement(1532..>2319)
                     /locus_tag="VLA60_04995"
     CDS             complement(1532..>2319)
                     /locus_tag="VLA60_04995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF08747.1"
                     /translation="TSFLLVSLLIMLFLYLPVMLLIGLSFNASTMGVAWKGFTLQWYE
                     KLVMDPAILHATLNSVVIAGVSTGLALVLGVGTALGLEKARGTQMRSVNMVMVLPLVI
                     PEILLGVALLMVFVLCRIPLGFGTIIIGHMVFNLPLTVVIIRARLRKLDPAWEDAARD
                     LGATSWEVLTRITLPLLRPAIWGAALLGFTVSLDDFVVTFFVAGPGSTTLPLKVFSMI
                     KTGITPEINALSAVLVIVSMICVGLSWLFQQRSTPSSSGSLYS"
BASE COUNT          621 a          664 c          548 g          486 t
ORIGIN      
        1 cttgatcatg gacagcgtct ccaccgcatt aaaccggacc cggggccccg gcatcttaag
       61 tgctttcact aaataaggaa cggaatgtct tccgtaatgg acaaattctc cgatggccca
      121 aaattcctgc cgcccctcga ggagaggaat aagcgattcc aatcgttcct gatcctgcag
      181 cgtgaggacc tcaggagccg gagtcgcttt ttctccggga gtatattccg gctgtgaagg
      241 aggctcatag ggaagttgca ccagataccg gtattgatct tcaaaacact gttccgcccc
      301 taccggaacc ccgcctagca ggaaggctgc aaccatcgaa gacaaaaccc tgaaccggac
      361 tgtgattctc ctggacatag aataaccttt cgtggtctta ccttctcaca tcagtatcat
      421 ccatctttta gtctagtagc tgaaagccaa aacggtcaag ccaaacccta ctgcactttc
      481 aattctgtcc atgcccgatc ataatacctg atcgcctccg ccacatcttc catccactcc
      541 atccgcggaa gaatctcatc aggcggatag acggcatggt tgtctcgaat gtccgcagtc
      601 agccgattcc ggacgtgacg attggctgag gcaaacagga gtcgttgcga cgtcagaagg
      661 gcgacttcct catccagaag gaaattaatg aaatccattg ccagcgcttg attgggagaa
      721 ctggccaaca ccgccaggca atccgtccag attgttccgc cttcccgggg aatggcataa
      781 cgaatcgacg gccgctcacg catggccctg gcgaccggac caccccacgc atgcgccaac
      841 acgacttcac cggcgaccag aagctgatca aattgttcac ttgtgtaagc tttgactaat
      901 ggtttttgta cgatcaattt ttgtttggcc tgttggatag cctgaggatc cacactattt
      961 aaggaatatc ccaaggcccg aagcgctaat ccaaacactt cccgttcatc attcagcata
     1021 ctgatccgac cggaaaattg aggatcccat aacgcctccc agctcgttgg cggtttcgtc
     1081 accacctccg cgttataccc gatccccaca gttccccata aataggggat ggcaaaacgg
     1141 tgcgtagggt caaacgggag gttttgcagg tgtggctcca ggtgttgcac attgggaatt
     1201 ttgttaaggt ccaggtcagc gagtaatccc aggcgcccca tcaaaccggc cataaaatcg
     1261 gaaggtacga tcacatcata gccggtcatg ccggtttgca cttttgccag caattcctca
     1321 ttgctgctga aggtgtccac cacgactcga actccccggc ttgtttcgaa tttcgctaca
     1381 atatcgggag ccacataatc cgaccaggtg aaataataca gagtaggacg tcgggaaccg
     1441 tctggttcag atgaggtgga ggattccgaa catcctccca ggagcgcaag ggtcaagaac
     1501 atcatcccca tcttcatcag tggcgggatt ctcatgaata caaggatccg gaagaagacg
     1561 gagtcgaccg ttgttgaaag agccaggaca atccgacaca gatcatcgaa actatcacca
     1621 ggacagccga gagagcattg atttccgggg taatccccgt tttgatcatc gaaaaaactt
     1681 ttagtggtaa cgtcgtggac ccagggccgg ccacaaagaa ggtcaccaca aaatcatcca
     1741 gtgacaccgt aaatcccaac agcgccgctc cccagatagc aggccgcagc aacggaagcg
     1801 tgatgcgggt gagcacctcc catgaggtgg cgcccaaatc ccgtgccgcg tcttcccagg
     1861 caggatctaa tttccgaaga cgggctctga tgatcacaac cgtcagcggt agattaaaca
     1921 ccatatgccc gataataatg gtaccaaaac ccaagggaat ccggcacaac acaaacacca
     1981 tcaataacgc cacacccaat aaaatttccg gaatcaccag cggcaacacc ataaccatat
     2041 tgaccgagcg catctgtgtt cccctggctt tttccaatcc gagggccgtc cctaccccaa
     2101 gaacgagagc caggccggta gacaccccgg cgatgaccac actgttcagg gtcgcatgga
     2161 gtatcgctgg atccatcacc agcttttcat accattgaag ggtgaaccct ttccaggcca
     2221 ctcccattgt ggaagcatta aaggacaacc cgatcaacag cataaccggc aggtacaaaa
     2281 acagcatgat cagcagactg acaaggagaa agcttgttg
//
LOCUS       DATIAL010000159         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_565944, whole genome shotgun sequence.
ACCESSION   DATIAL010000159 DATIAL010000000
VERSION     DATIAL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565944"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..2219)
                     /locus_tag="VLA60_05000"
     CDS             complement(105..2219)
                     /locus_tag="VLA60_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08748.1"
                     /translation="MKDTQLTENKPKLESMASGNTSGELAQELVASEERFQSVVQTAT
                     DAIILWDEGGNILFWNKGAQTMFGYAEAEVVGKPLTLLIPLRYHEAHHYGMGRVGTEG
                     EEGRATVKTVELHGLRKNGDEFPIEMAPSKSILEGETFFCGIIRDISERKQADQVLEE
                     RNRLLAFEVEVGHVLNRSQALGVLWQGCVDAMVRHLGAALAGIWTLDSQQEGLELQAS
                     AGLYIHVTGVQGPMPFGHLQIGQIAFQKRPYLTNAILEDPGVPEQAWAKQEGLVAFAG
                     YPLLINREVAGVMAMYFQHPLTNFTWHSLGLVADRIATAIERQKVIDANQRLARHNER
                     ILASSGEGMLGLDGEGKMTFVNPAGAQMLGYKREEMVGESMHELIHHTNSDGSPCLRE
                     SCPIYAALKEGSVHQNDTEVFWRKDGTSFPVDHYISPIWENGSLIGAVVTFKDISERK
                     RLTAELLEETKLASVTVMLGDIAHDIKNMLMPVLNGAKLLDDDLQELFTSVPDVTPKQ
                     VTASRKFSREAIDMIVNNARRIQGRMREIADTVKGISSPLRFAPCRISDVVKGVFDIL
                     RFYATEKGVSLQAQGLDALPSIHADENRVFNALYNLVNNAIPETPAGGSVTIGGSVGS
                     DRKAVILTVTDTGGGMPPEIRDRLFTKEAISGKAGGTGLGTKIVKDAVDAHSGTITVH
                     SEQGTGSIFTIHLPITPQILEP"
BASE COUNT          617 a          712 c          619 g          661 t
ORIGIN      
        1 cctatttgga tggacggacg tatcgtaggt tacgcacaac gaaggcaagg aaaggtgctg
       61 gcaaatctta cacctggctc agaatcctgg tagtggaaat tccttcatgg ttcaagaatt
      121 tgcggggtaa tcggtaaatg aatggtgaag atgctacctg tcccttgttc actgtgcacg
      181 gtgatcgttc cggaatgagc atccaccgca tccttaacaa tttttgtccc aagtccggtt
      241 cccccggctt tcccactaat cgcttctttg gtaaataatc tgtcgcggat ttcagggggc
      301 attcccccac ccgtatcagt gaccgttaaa attacagctt ttctatctga tcctactgac
      361 ccaccgatgg tgactgatcc tccggcaggc gtttccggga ttgcattgtt gaccaaattg
      421 taaagcgcat tgaacacacg attctcatca gcatgaatgg acggaagggc gtcaaggcct
      481 tgtgcttgca gagaaactcc tttctcggtg gcatagaacc ggaggatatc aaaaacacct
      541 ttaacaacat ccgaaatccg gcaaggagca aaccggagag ggctggatat ccccttcacc
      601 gtatcggcaa tctcccgcat gcgaccttgg atccgacggg cgttattgac aatcatgtca
      661 atggcctctc tggaaaattt tctggaggcc gtcacttgct tcggggtgac gtcaggcacg
      721 ctcgtaaaca gctcctgaag atcgtcatct aacagcttgg cgccattcag caccggcatg
      781 agcatattct taatatcatg ggcaatatcc cccagcatca ccgttacgct ggccagtttc
      841 gtttcttcca gcaattcggc cgtcaagcgt tttcgctcac tgatgtcttt gaatgtcacc
      901 acagccccga ttaaacttcc gttttcccat atcggcgaaa tgtagtggtc aacagggaaa
      961 gaggtgccat ccttacgcca gaacacctct gtgtcattct gatgaacgct gccctcctta
     1021 agcgcggcgt aaatgggaca ggattctctg agacagggag acccatcgga gttcgtatga
     1081 tgtataagct catgcatcga ctcgccgacc atttcctctc ttttgtatcc caacatctga
     1141 gccccggcag gattgacaaa ggtcatcttg ccttctccat ccaaccccaa cattccttct
     1201 cccgatgaag ccagaatgcg ttcgttgtgc ctcgcaaggc gctggttggc atctataact
     1261 ttttgacgtt cgatggccgt ggcaattcgg tccgccacta aaccaagact gtgccacgtg
     1321 aagttcgtca agggatgctg gaaatacatc gccataacac ccgccacctc ccggttaatg
     1381 agcaacgggt accccgcaaa ggccaccagc ccctcctgct tggcccaagc ctgctccggg
     1441 acgccaggat cttcaaggat cgcattggtc aaatacggtc ttttctgaaa ggcaatctgc
     1501 ccaatttgaa ggtgtccaaa tggcatcggg ccttgcacac ctgttacatg gatgtaaagc
     1561 ccggcactgg cttggagttc cagcccctct tgctgggaat caagcgtcca gatcccggcc
     1621 aacgccgcac cgaggtgtcg aaccatagca tccacacagc cttgccagag gactcccagt
     1681 gcttgactgc gattgagcac atgccccact tctacctcaa aggccagcag gcggttccgt
     1741 tcctccaaaa cctgatctgc ctgctttcgc tcagaaatgt ctcggatgat cccacaaaaa
     1801 aaggtctcac cctcgaggat tgattttgac ggagccattt caataggaaa ctcatccccg
     1861 ttctttcgta acccatgcag ttcaactgtt tttacggtgg cccgtccctc ctccccttca
     1921 gtaccgaccc gccccatgcc ataatgatgc gcttcatggt agcggagagg tatgaggagg
     1981 gtgagtggct ttccgacgac ttccgcctca gcatagccaa acatggtttg ggcaccttta
     2041 ttccaaaaaa gaatattccc tccctcatcc cacaaaataa tggcatccgt ggcggtctgt
     2101 acgacggact gaaaccgttc ttcgctggca accaattcct gagccaattc cccggatgtg
     2161 tttccggaag ccatggactc taatttaggt ttgttctctg tcaattgagt gtctttcatt
     2221 tattgccttc tgaacattta ttaaaaatct taactggatt caactcgcta gtacagcgaa
     2281 tgaagtgata ccacaatctt cgtgatatgg gctgtaaacc ccaaagcttt tctccagcga
     2341 tgggctgagt tgggacataa cgagacgagt ggctttccta gtacgtcgtg aaggatctgc
     2401 gttccgggaa taccgatgaa tcaggccatt gatggtttca tgagcaaccg cgttcgcacg
     2461 tgtatttagg acactggttc agaggctgac gcgagattaa cgctcgagga gggagagcgg
     2521 atggggtgat ggagttcttg ccgatcatcg gaacgtgagg ccaatcctgg agggttttca
     2581 taactgttac ctggtatata ttcaacttt
//
LOCUS       DATIAL010000160         2173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_566055, whole genome shotgun sequence.
ACCESSION   DATIAL010000160 DATIAL010000000
VERSION     DATIAL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566055"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VLA60_05005"
     CDS             complement(<1..235)
                     /locus_tag="VLA60_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSF08749.1"
                     /translation="MSRIDLHTHTHFSDGSVSPTALVELAHQQGVSILAITDHDTTEG
                     LPEAMEAAHHLPIEIIPGIELSTEFQGRETHMLG"
     gene            complement(251..1162)
                     /gene="folB"
                     /locus_tag="VLA60_05010"
     CDS             complement(251..1162)
                     /gene="folB"
                     /locus_tag="VLA60_05010"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HSF08750.1"
                     /translation="MSSSIVLKGIQLAARCGVTEAERQQPQPLLVDLIFRCPNQSAFQ
                     SDQISDTVDYGTITQRIQDIGEGQAFSLIETLAERICQALLHEFPITRLKIWVRKIRP
                     PLKSIEGSVGVQLIRSRKPIASLDHKHLSPFLVDQLSRIPHGTVLDVATGRGRHAMYL
                     ASKGFSVHGIDRDADALHELQEQAQEADLSSITTECIDLEANPQHPPDLGTAVYDVII
                     VFFYLYRPLFPQLVNALKSGGVIMYETFLLDNHIHRQHPRRKEFCLETNELLTLLQGL
                     RILHYDEGDHVSSSGRAFTARILARKP"
     gene            1281..1739
                     /locus_tag="VLA60_05015"
     CDS             1281..1739
                     /locus_tag="VLA60_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSF08751.1"
                     /translation="MKFGKWIFRQRWIRRKAGPRFGWIIGCLSCLVLQGSCEWGGSST
                     IMVTAEEFRFNPTRIEWVSDQPLRLLIRNQGRERHVFHSRELFGPEAGVLWHQPKVAL
                     QEANAVVLEPGQSIELSFTASAGLYPFRCWIKGHTGMEGTILVKNRSSKL"
     gene            complement(1933..>2173)
                     /locus_tag="VLA60_05020"
     CDS             complement(1933..>2173)
                     /locus_tag="VLA60_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08752.1"
                     /translation="GATGASGSAASPASPSSNAEAKAVTTGPTRVSTGTQGDTLEACL
                     SRIPSDATAGQKMLATMSCERDAKARTSIDAVPGK"
BASE COUNT          490 a          499 c          628 g          556 t
ORIGIN      
        1 agccgagcat atgcgtttcc cgcccctgaa actctgtact taattcaatg ccgggtatga
       61 tttcaatcgg gagatgatga gcggcttcca tggcttccgg caggccctca gtcgtgtcat
      121 ggtcggtaat ggccaggatg ctcacgcctt gttgatgagc aagttccacg agtgcggtgg
      181 gcgaaacact gccgtccgag aaatgggtgt gagtatgcag gtcgatacgg ctcatgagat
      241 cggttgagtt ttatggtttc cgtgccaaaa tgcgtgcggt aaacgcccgt cctgaagagc
      301 ttacatgatc cccttcgtcg taatggagaa ttctgagccc ttgaagaagc gtgagcaatt
      361 cattggtttc caggcagaac tcttttcggc gtgggtgttg ccgatgaata tggttgtcca
      421 gaaggaaggt ctcatacatg atgacgccac cggatttcag ggcgttaacc agttgaggaa
      481 agagcggccg gtacaagtag aagaagacga taatcacatc gtacacggct gtgcccaagt
      541 ccgggggatg ttgcggattt gcttctaagt cgatgcattc ggtcgttatc gaggagaggt
      601 ccgcttcttg ggcttgctcc tgaagctcat gtagcgcatc ggcatcgcga tcgatgccat
      661 gaaccgaaaa ccctttggaa gccagataca tggcatgacg accacgtccc gttgccacat
      721 ccaacaccgt tccgtgagga attcgggaga gctggtctac tagaaatgga gaaagatgct
      781 tgtgatcaag ggaggcgatt ggtttgcgcg atcggattaa ttggaccccg acagacccct
      841 caatgctctt caacggaggg cggatttttc tgacccatat tttgagacgg gtaatgggaa
      901 attcatgaag cagggcttgg cagattctct ccgccaatgt ttcaatcagc gaaaaggcct
      961 gcccttcccc aatgtcctga attcgttgtg tgattgttcc gtaatcgacc gtatcggaaa
     1021 tttgatcact ctgaaaggcc gattgattgg ggcatcggaa gataagatcg accagaagag
     1081 gttggggttg ctgtctttcc gcttcagtga ccccgcatcg tgcggccaac tggatacctt
     1141 tgaggacgat agatgaagac atgggccatt atgtcagaac ttccttgtcg tgtgcatgtg
     1201 atttttaaaa ggaggcgatt cggagtccgg atcgtgttcc gaaatagggg gcttggatgg
     1261 gtttcattcg ggaggtattg atgaaattcg gtaaatggat cttccgccag cggtggattc
     1321 gtcgaaaggc cggccctaga tttgggtgga tcatcggttg cctctcttgc ctcgttctcc
     1381 agggatcgtg tgaatggggt gggtcatcca ctatcatggt gacggctgaa gagtttcgat
     1441 ttaatcctac ccggattgaa tgggtatccg accagcctct tcgtcttctg attagaaatc
     1501 agggacggga acgacatgtg tttcacagcc gggaattgtt cggaccggag gctggcgtcc
     1561 tatggcatca gccaaaggtg gcgcttcaag aagccaatgc cgttgtcctt gagcctggtc
     1621 aatcgattga gttgtctttc accgcgtctg ccggtctcta tccctttcgc tgttggataa
     1681 aagggcacac cggcatggag ggcacgatcc tcgtcaaaaa tcgttcttca aaattatgaa
     1741 tgctttatcg aggagatcac aaaaaggtcg gcagacaacc gcaacgcttc ggatttttta
     1801 ttggcttagg tcggaatttt aagaacctca aagatctgag ggacttcgat tgtctggcga
     1861 aatttagaaa tacgaatgga gacgactggc agaatgcttg ctggtgtcag tcagtcacca
     1921 gcaagcgatg ttttatttgc ctgggacagc gtcaatggag gttctggcct tggcgtcacg
     1981 ttcgcaactc atcgtggcga gcattttctg gccagctgtc gcatcactcg gaattcggga
     2041 aagacaggct tcaagcgtgt cgccttgcgt gccggtggag actcgagtcg gtccggtcgt
     2101 cacggcttta gcttcagcgt ttgacgatgg tgaagccgga gaagctgccg agccacttgc
     2161 accagttgcg ccg
//
LOCUS       DATIAL010000161         6223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_351746, whole genome shotgun sequence.
ACCESSION   DATIAL010000161 DATIAL010000000
VERSION     DATIAL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351746"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VLA60_05025"
     CDS             complement(<1..566)
                     /locus_tag="VLA60_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSF08753.1"
                     /translation="MLYALIPLLPLLAFVILGLFGHWIKDRSHWVAVPAVLGSFLLSI
                     MALVDVAGGQTLQIPLYTWADSGNLHIALGLFIDQLTVAMLLLVTIVSSLVHVYTIGY
                     MHGEPGYARFFSNIALFTFSMLMLVMSDNFLQLFVFWEAVGLCSYLLIGHWYERESAR
                     AAATKAFLVNRIGDFGFMLGILLIFVTFG"
     gene            complement(571..876)
                     /gene="nuoK"
                     /locus_tag="VLA60_05030"
     CDS             complement(571..876)
                     /gene="nuoK"
                     /locus_tag="VLA60_05030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HSF08754.1"
                     /translation="MDVPVSYYLVLSGIVFSIGLVGVLIRRNIIIILLSIELMLNATN
                     INFVAFSSYLGNLSGQVFVFFALTVAAAEVAVGLAIIIALYRHSASTNIDDFRLLKW"
     gene            complement(876..1394)
                     /locus_tag="VLA60_05035"
     CDS             complement(876..1394)
                     /locus_tag="VLA60_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSF08755.1"
                     /translation="METIVFFYFASVIVVTAALVVALRSPVFSALALLVMFFHVAGLF
                     VTLHAEFLAVIQILVYAGAILVLYLFVVMLLNLKGEVHFHHQLTVGLFLGFMVFAEAV
                     LLVLKGESTLKASLSTPREPAQLMQGNTESIGELLYSTYLFPFEIASLILLVAMVGAV
                     ILTKKGILEAKG"
     gene            complement(1470..2009)
                     /gene="nuoI"
                     /locus_tag="VLA60_05040"
     CDS             complement(1470..2009)
                     /gene="nuoI"
                     /locus_tag="VLA60_05040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSF08756.1"
                     /translation="MKLKEWVKTLLFYEIALGMKATLSHFLHYKPITLQYPHEKKQLP
                     SNYRGMLALLRYDDGTEKCVGCDLCEAACPSRVIRVVSAEVPGEPTKRYSKEYYMDMT
                     RCLFCGLCVKACPVDALGMTQEYEWAVYDKRQLQLNKEQLLAIGDRNFPVREKRLEFQ
                     HPNVAYFNVGFKEIPQKEL"
     gene            complement(2024..3100)
                     /gene="nuoH"
                     /locus_tag="VLA60_05045"
     CDS             complement(2024..3100)
                     /gene="nuoH"
                     /locus_tag="VLA60_05045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HSF08757.1"
                     /translation="METVLIRLGLTLSQIVVVMVAVLLTVAALTLLERKVLGWMQDRM
                     GPMEVGPYGILQPVADGLKLFFKEDIIPAGANKFMFSMAPVLSLVPALIGFAVIPFGP
                     DWTVDVGGVNFKPFVITDINIGVLYILAFASIGAYGIILGGWSSNSKYSLLGGLRSAA
                     QLISYELNVGLSIVGVLLLSSSLSLVTITNAQAGGFWNWYFFGGGGFPQALAFIVFII
                     SAVAETNRTPFDLPEAESELVAGFFTEYSGMRFAFFFLAEYGNMILVSCVATVLFFGG
                     WHPPYPGTLMESIGMGHLLWIEGIMWFVLKVAFFLFFFFWIRATLPRLRYDQLMRFGW
                     KVLLPIALANIVVTSIVAFLFPGS"
     gene            complement(3152..5836)
                     /gene="nuoG"
                     /locus_tag="VLA60_05050"
     CDS             complement(3152..5836)
                     /gene="nuoG"
                     /locus_tag="VLA60_05050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HSF08758.1"
                     /translation="MATTPTTDMITLTIDGKSATVAKGTLVIEAARQVGVMVPHFCYH
                     PKLTPDANCRMCLVEIEKMPRLQTSCSTQVAEGMVVKSASSSVVQDARKSVLDFILAN
                     HPLDCPVCDQGGRCDLQDFSHEYTATTSGFKELKRVFPKEYFSPLIETQMNRCVQCLR
                     CVRYCDQVMDVKALAPVGRGTMTEIKAFGAHELDCEFCGGCIQICPVGAITSRVSMYE
                     FRPWMLKRTDSVCGYCGDGCQITFQTKNNDLIEVNSTHGAGRNNGDLCARGYFGYHVS
                     VHPDRLTAPLIRRGDAFHPVQWEEALELVGQRLQEIKTTYGPDAIGGLITGRCTNEDI
                     YVFQKFMRGVVGTNRIDSSVRYGHLNAVRAMQRVQGTYRWAVNFEDILSADTLLLVGT
                     NITETNPITGLKVKEAVKRRGAKLITIEALEPAIGSISNIVNLAHHHLGVKPTAYAGA
                     VLGILKAVVDQQMVDPELQANARSYANDMTARLEALSWEAIQAQTGLASDMFTQAAQM
                     FAKGQKVVILVGPGVLRSVGGFGMTTNLLDLLLVTGKLTKPGCGLAPLAEENNDQGAF
                     EMGGTGSVLPGGQSLGDPDVRKRWAQAWGRAIPETPGSSLPQMIEDAQHGKLKALFVV
                     GENPLASLPPSSGIFSALEKLEFLVCQELFLTETAQQAHVVLPACSYAEKDGTFTNTE
                     GHIQSVRKAIDLVGESRPDWEVFSALSVMMNDPMEYGDVKEIGKEIHNLLPGTRTLGP
                     APLPAQPDSTVIARYLKADYQRDIATRYQLPADDAAVGAEDMILQVSQSLFHSGKFSR
                     KAKGLLQVEATGKLYLHPEDAARRGIGEGDTVKVSNRLGEVITPVGLRERIPQGVVMF
                     PEHFDEEVRRLLSYSVDLETQVPYYKVACVRVEKVVGT"
     gene            complement(5848..>6223)
                     /locus_tag="VLA60_05055"
     CDS             complement(5848..>6223)
                     /locus_tag="VLA60_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HSF08759.1"
                     /translation="AGSMLGSGGVTVMEEGTNMVWAALRLMEFFYHESCGKCTPCREG
                     TSWLVQTLQRIWNKRGRPEDIGVLDELCGNIPGRTVCAFGDAAVSPIVSTLHHWRGEY
                     EALIREAQETMPRNKEIPVFTH"
BASE COUNT         1698 a         1788 c         1419 g         1318 t
ORIGIN      
        1 ccgaaggtga caaagatcag cagaataccc aacatgaatc cgaaatcccc gattcgattg
       61 accagaaacg ctttggtggc cgctgccctg gctgactcgc gctcatacca atgcccgatt
      121 aacaggtagg agcaaagacc cacggcttcc caaaagacaa acagttgcag gaaattatcg
      181 gacatcacca acatcaacat ggaaaaggta aaaagcgcga tattgctaaa aaatctggcg
      241 tagcccggct ctccatgcat gtagccaatc gtatagacat ggaccagcga actcacaatg
      301 gtgacaagca acaacatcgc aaccgtgagc tgatcgatga ataaccctaa ggcaatatgc
      361 agatttcccg aatccgccca cgtatagaga ggtatctgaa gcgtctgacc acccgccacg
      421 tcgacaagcg ccatgataga aagaaggaag gatcccagga cggctgggac ggccacccaa
      481 tggcttcggt ctttgatcca atggccgaac agaccgagga tgacaaaggc caggagtggg
      541 aggagaggaa ttaaggcgta gagcatcgcc ttaccatttc agcaaccgga agtcgtcaat
      601 gttggtactg gccgaatgcc gatagagcgc gatgataatc gccagcccta ccgcaacctc
      661 cgcagcagcc acggtaagcg caaaaaacac gaacacctgt ccggaaagat tccccaaata
      721 ggaagaaaag gccacaaaat taatattcgt ggcattcaac atcaactcaa tggataataa
      781 aataatgatg atattgcgac gaatgagcac tcccaccaac ccgattgaga acacgattcc
      841 gctcagcaca aggtagtagc tcactggaac gtccattaac ccttggcctc caatatcccc
      901 tttttggtga ggataaccgc acccaccatc gccactagga gaatgagaga cgcgatttca
      961 aatggaaata gatacgtgga atacaggagc tcaccgatcg attccgtatt gccctgcatc
     1021 agctgagcag gctccctggg agtggaaaga ctggccttca atgtggattc gcctttcagc
     1081 accagcagga ccgcctctgc aaagaccata aatcccagaa agaggcctac ggtcaattgg
     1141 tggtggaaat gtacttcacc cttcaaattg agcaacatga ccacaaagag ataaagcacc
     1201 agaatggcgc ccgcatacac caggatttga atcacggcca ggaattccgc atgcaacgtg
     1261 acaaaaagcc cggcgacgtg aaagaacatg accagaagcg ccagagcact aaacaccgga
     1321 ctcctcaggg ccacgacgag agccgccgtt accacaatca cactggcaaa atagaaaaaa
     1381 actatcgttt ccatcaccgg ttcattcaaa aattttggcg atattctttg gccggcatgt
     1441 ggagcgagtg caccgccagg ccatatcgac tacagttctt tttgcggaat ctctttaaac
     1501 cctacgttaa aataggccac attgggatgt tgaaactcaa gtcgtttttc ccgaactgga
     1561 aagttgcgat cgccaatagc cagaagctgc tccttattta attgtagctg gcgcttatca
     1621 tagaccgccc attcatactc ctgagtcatt cccaacgcat ccaccggaca tgccttcacg
     1681 cacaacccgc aaaacaagca tcgggtcatg tccatgtaat attccttgga ataccgcttc
     1741 gtgggttccc ccggcacctc ggcactcacc acacggatga cgcgggaggg acaagccgcc
     1801 tcacataaat cgcatcccac gcacttttcc gtcccatcat cgtagcgcag caacgccaac
     1861 atgccgcgat aattggacgg caactgtttc ttctcgtgcg ggtattgtaa ggtgatcggc
     1921 ttataatgaa ggaaatggga taaggtcgcc ttcatcccta acgcaatctc ataaaataaa
     1981 agcgttttga cccattcctt gagtttcata ccattcaacc ttcttagctc ccaggaaaga
     2041 gaaatgcaac aatcgacgtc accacaatat tagccaacgc gatgggcagg aggaccttcc
     2101 acccgaatcg catgagctga tcatatcgta atcgcggtaa tgtggcccgg atccaaaaaa
     2161 agaaaaagag gaaaaacgcc actttgagga caaaccacat gatgccctca atccagagga
     2221 gatggcccat cccgatggac tccataagcg tgcctggata gggaggatgc cagccaccaa
     2281 aaaacaagac cgtggccacg caagacacca aaatcatatt gccgtattcg gccagaaaga
     2341 agaaggcgaa tcgcatgccg ctatattccg taaagaatcc ggccaccaac tcactttccg
     2401 cttccggcaa atcaaacggc gttcgattcg tttccgctac tgcagaaata ataaacacga
     2461 tgaacgccag ggcttgaggg aacccccctc ccccgaaaaa gtaccagttc caaaatcctc
     2521 cggcctgcgc attggtaatc gtcacgagac tcaaggagct tgaaaggagc aacacgccga
     2581 caattgaaag tcccacattc aattcgtaac taatcaactg ggccgcagac cgcaggcctc
     2641 ccagaaggga atacttactg tttgatgacc acccgcccaa aataatgccg tatgccccga
     2701 ttgatgcaaa cgccaggata tagagaacgc cgatattgat gtcggtaatc acaaagggtt
     2761 taaagttgac gccgccgaca tcaaccgtcc aatccggacc aaacggaatc accgcgaatc
     2821 cgatgagggc aggaactaat gacaacaccg gcgccatgct aaacatgaac ttattcgctc
     2881 cggctgggat gatgtcttct ttaaaaaaga gctttaatcc gtcggcgacc ggttgcaaga
     2941 tgccataagg cccgacttcc atgggcccca tccgatcctg catccaaccc aacaccttcc
     3001 gctccaggag ggtgagtgct gcgacagtca gaagcaccgc caccatcacg acaacaattt
     3061 gagataatgt cagccctaaa cggattagta ccgtttccat gcctgattca ctcctctcct
     3121 ctatatatca acacacaggc tgagaaccgc ctcatgttcc caccactttt tcaacacgaa
     3181 cgcaagccac cttgtaatag ggcacctgcg tttccaaatc aacactatac gacaagagac
     3241 ggcgaacctc ttcatcaaaa tgctcgggga acatcacgac tccctgcggg attcgctcac
     3301 gaagaccgac cggggtgatc acctcaccca aacgattgct caccttcacg gtgtcccctt
     3361 caccaatacc acgtcgcgcg gcatcttccg gatgcagata gagtttgccg gtcgcttcaa
     3421 cttgcagtag gcctttggct tttcgcgaaa atttcccgga atgaaacaag gattgcgaaa
     3481 cctgcaagat catgtcttcc gcaccaacag cggcatcatc tgcgggcaat tgataccgag
     3541 tagcgatatc ccgctgataa tcggccttga gatatcgggc gatgaccgtc gaatccggtt
     3601 gagcgggcaa gggggcaggg cccagggtac gcgtaccggg gagaaggtta tggatctctt
     3661 tgccaatttc cttgacgtct ccatattcca tcggatcatt catcatcacc gacagcgcgg
     3721 aaaacacttc ccaatccggt cggctctcac caaccagatc aatggctttt cgtactgact
     3781 gaatatgccc ttcggtgttg gtaaacgttc cgtccttttc cgcataggaa caggctggca
     3841 gaacgacatg agcttgctgg gccgtttcgg tgagaaacaa ttcctgacac accagaaact
     3901 ccagcttctc caatgccgaa aaaatacccg aggacggagg caaggaggcc aggggatttt
     3961 ccccaaccac aaacaaggcc tttagtttgc cgtgttgcgc atcttcaatc atctgtggga
     4021 gtgaagagcc tggtgtttcg ggaattgccc ggccccacgc ttgcgcccag cgcttccgca
     4081 catccgggtc acccagggat tgtccaccag gcaataccga ccccgttcct cccatttcaa
     4141 aggctccttg atcattattt tcttccgcca ggggagcaag gccacagccc ggcttggtga
     4201 gttttcccgt taccaataac aggtccaaca aattcgtggt cattccgaat ccacccacag
     4261 atcgcaggac tcccggtccg acaagaatca cgaccttctg tcctttggca aacatctggg
     4321 cggcctgagt aaacatatcc gaagccaatc cggtttgcgc ttgaatcgcc tcccaggaca
     4381 gggcttccag tctggcggtc atgtcgttgg cataagaccg ggcgttggcc tgaagttccg
     4441 gatccaccat ttgctgatca accacagctt tcaggatacc caaaaccgcc ccggcatagg
     4501 ccgtcggttt gacgcccaga tgatggtggg ccagattcac aatattgctg atgctcccaa
     4561 tagcaggttc caacgcttca atggtgatga gctttgcccc tcgccgtttc accgcttctt
     4621 tgaccttcaa ccctgtaatg ggattggttt ccgtaatatt ggtcccgaca agtaagaggg
     4681 tatctgcgga gagaatatcc tcaaagttca cggcccaacg ataagtgcct tgcactcgtt
     4741 gcatggcacg caccgcattc aggtgtccat accgcacact actatcaatc cgattggtcc
     4801 ccaccacgcc gcgcatgaat ttctgaaaaa catagatatc ttcattcgta catcgtcccg
     4861 taattaaccc gccaatcgca tcgggaccat acgtggtttt tatttcctgt aaacgctgcc
     4921 ccaccaactc caaagcctct tcccattgca cagggtgaaa ggcatcccct cgacgaatga
     4981 gaggcgccgt cagccgatca ggatgaacgc tgacatggta gccaaagtag cctcgggcac
     5041 agagatcacc attattgcgg ccggccccgt gggtggaatt cacttcgatt aagtcattat
     5101 tcttggtttg aaaggtaatt tggcacccat ctccacaata gccacaaacg gaatcagtcc
     5161 gcttcagcat ccatggacga aattcataca tagagactcg actggtaatg gctccgaccg
     5221 gacaaatctg gatacatcca ccacaaaact cacaatccag ttcatgggcc ccgaaggctt
     5281 taatttccgt catggtgccg cgtcccacag gcgccagggc tttcacatcc atgacctgat
     5341 cacaatagcg cacgcatcgc aaacattgga cacaccggtt catctgcgtt tcaatcagcg
     5401 gactgaaata ctccttcgga aagacccgtt tgagttcctt aaatccgctg gtcgtcgctg
     5461 tatattcatg ggaaaagtcc tgaagatcgc agcgcccacc ttgatcgcaa acgggacaat
     5521 ccaatgggtg attggccaaa ataaagtcca acaccgattt gcgggcgtcc tgcaccaccg
     5581 aggaagaagc cgatttcacc accatacctt ccgccacttg cgtactacag gaggtttgca
     5641 acctgggcat tttttcaatt tccactaaac acatccggca attggcgtcg ggcgtcagtt
     5701 tcgggtgata gcaaaagtgc ggcaccatga caccaacctg ccgcgcagcc tcaatgacca
     5761 gcgtcccttt ggcaaccgtt gccgattttc catcgatcgt cagcgtaatc atgtctgtgg
     5821 ttggagtagt ggccattttg gttacactca atgcgtgaaa actggaattt ccttgttgcg
     5881 aggcatcgtc tcttgagcct cacgaataag ggcctcatat tccccacgcc aatggtgcag
     5941 cgtgctcaca attggggaga cggccgcatc accaaacgca caaaccgtcc ggccagggat
     6001 atttccacag agttcatcaa ggactccaat atcttcagga cgccctcgtt tgttccagat
     6061 ccgctgaaga gtctgaacca gccaggaagt tccttcccgg cagggtgtac attttccaca
     6121 cgattcatgg tagaaaaatt ccatcaatcg cagggcagcc caaaccatat tcgtgccttc
     6181 ttccatcacc gtgacaccgc cggatcccag cattgacccc gct
//
LOCUS       DATIAL010000162         5051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_782062, whole genome shotgun sequence.
ACCESSION   DATIAL010000162 DATIAL010000000
VERSION     DATIAL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782062"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VLA60_05060"
     CDS             complement(<1..423)
                     /locus_tag="VLA60_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSF08760.1"
                     /translation="MAIRLGDEAPNFTAETTQGTVNFHEWLGDGWGILFSHPKDFTPV
                     CTTELGTMANMTEEFKKRNVKVLAVSVDPLDSHKGWIKDINETQHCNVSYPIIADPER
                     KVANLYDMIHPNALDNMTVRSVFVIGPDKKVKLTLTYPA"
     gene            654..1142
                     /gene="purE"
                     /locus_tag="VLA60_05065"
     CDS             654..1142
                     /gene="purE"
                     /locus_tag="VLA60_05065"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSF08761.1"
                     /translation="MAESSLQVAILMGSKSDWDTMRLASETLSQFHIPHESRVLSAHR
                     APDALVDYIRQAEGRGVQVFIAGAGGAAHLAGVIAAKTQLPVLGVPMQSKALQGLDSL
                     LSIVQMPKGIPVGTLAIGSAGAVNAALLAVAILALADSSLSKRLQGYREEQTRAVLAE
                     QL"
     gene            complement(1356..1610)
                     /locus_tag="VLA60_05070"
     CDS             complement(1356..1610)
                     /locus_tag="VLA60_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HSF08762.1"
                     /translation="MIKPGRYRHYKGKEYQVIGVAKHSETEEDLVVYRALYGTYGLWV
                     RPVAMFCEKIQNGEKVVFRFECIEEANGEAITPSKSPAEG"
     gene            complement(1669..2469)
                     /locus_tag="VLA60_05075"
     CDS             complement(1669..2469)
                     /locus_tag="VLA60_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSF08763.1"
                     /translation="MNQPHSISSRKNSHFKRWLSLLDAHGIKRHHQCLVSGNKLRNEI
                     WKNSGHSAREFLFPSSWNIEEDLPCQTKSYILSHSLFKELDVFGTKEPLVVCNIPEIS
                     SMDLTQAPNGLEVLCPMGDPGNLGAFIRSCRAFDVQTVILLQEAVHPFHPKVIRTSSG
                     AVFVQSLKWGGSIANLNTAETLKWITALDLQGKNISTVKWPKNVRLLIGEEGVGVPCF
                     RFAERLCISQIHPSIPLNATVAGTIALFAYRQKNFTPFTSQEREAGLS"
     gene            2691..3851
                     /locus_tag="VLA60_05080"
     CDS             2691..3851
                     /locus_tag="VLA60_05080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSF08764.1"
                     /translation="MLGTWSIREKDQNQNDGSEKNPAANVSSATGASSEYDHALEALA
                     RILRSLGRHAFELDRLSEEAIEKEFEGWAMHVLVGARIPGEEEEKLEPSTRREWGSLS
                     RFVNGHRQQEKTYVTRNLQDMRNVIWEFTHIVGRAIVEDQEVDEKVINHLAQLRSTTE
                     GGSFQDVKQALCAVVQGISTLMDERKNRQQQRLKRLGEKLHAVEAELGSTRKQLTLDS
                     LTQLYNRGALDLQLERTASLSFFSGTPACILMVDVDHFKHINDTYGHPAGDVVLQHLA
                     KRLVATFPRKTDFIARYGGEEFCVLLQGAGLDLSQRLGERLLDAVRREKFHYQDIEIS
                     ATVSIGVAELRPGEAVPSWIERTDRALYRAKEGGRNQLCRALETKGVGQEIK"
     gene            complement(3882..4859)
                     /locus_tag="VLA60_05085"
     CDS             complement(3882..4859)
                     /locus_tag="VLA60_05085"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF08765.1"
                     /translation="MQQPLPLDMAQDEEISTIFERIKRCKSLPILSPLASRILKMCQN
                     EKTQTSELAHIISQDPGMAAQILFMANSGYYGGSRHKVTTIMQAVTLLGFSTIANLAL
                     SFCFYRLIKDMKEISTSGMDHVKFWRRSILASVAGRAIGKHFAYPDTELMFLGALLQD
                     IGILALNEVASKELTIFHSQYQSNHAALQQLELEHLGVDHQQVGAWLAQHWQLPEEFQ
                     IAILYSHTPELFETPADFQSLVDAVALSGIIADIWTNPDTDAAIALAGEKSQGRLTLQ
                     PHDWEPVLSHIMTGIPQIASFFHTRLGNFEEINHIHRLALKQLGTSKLP"
BASE COUNT         1403 a         1048 c         1259 g         1341 t
ORIGIN      
        1 tgcaggatag gtaagggtta atttgacctt tttatccggg ccgatcacaa aaacggaacg
       61 aacagtcata ttatccagag cattgggatg aatcatgtca taaaggttcg cgacttttcg
      121 ttcagggtcg gcaataatag gataactcac gttgcagtgt tgagtctcat taatatcctt
      181 gatccaacct ttatgagagt cgagtggatc cacactgaca gccagcacct tgacgtttcg
      241 ttttttaaat tcctccgtca tgttagccat ggttcccaat tccgtggtgc aaacgggggt
      301 aaaatccttg ggatgtgaaa aaaggatacc ccatccgtct ccgagccatt catgaaaatt
      361 cacggtgcct tgtgtcgttt ctgccgtaaa atttggtgcc tcatctccaa gacgtattgc
      421 cataatactt ctctcccgtt atgaaaacag tgaataatat atctctccgg tagaaaacac
      481 cgcgagaaaa gttgatgacc caaagagatt tcctgaaaat ttcaggtgga ttttagttta
      541 agatagttcc cttttgatgg tcaacttgag ttaggatgcg ctatcgtaag cgcgttcatt
      601 cctggaattt gcttggatca tcaacacgaa ataatggagg agcaaaagaa attatggctg
      661 agtcatcatt gcaagtggca attcttatgg gaagtaaatc ggattgggac accatgcgat
      721 tagctagcga gacactatct caatttcaca ttcctcatga gagccgtgtt ctttccgcgc
      781 atagggctcc cgatgccttg gtggattata ttaggcaagc agaagggcgg ggggtgcagg
      841 tgtttattgc cggagctgga ggagctgcac atttagccgg agtcatcgcg gcgaaaaccc
      901 aattgccggt attaggtgtt cccatgcaat cgaaagcctt acaaggtctc gattcacttt
      961 tatcgattgt ccaaatgcca aaaggcattc cagtgggaac gttggcgata ggaagcgcgg
     1021 gcgcggtgaa tgcggccttg ttggccgtgg ctatcctagc cttggccgat tcctcattat
     1081 ccaaacggtt gcagggctat cgagaagagc aaactcgagc cgtgttggca gaacaactgt
     1141 aaggccgatg gttaaaatcc gggatcattt atcgccgagt gataggtgac agcttcactg
     1201 attcatggga ttaaatggaa tctccacacg aatagatttt tttataggag tgtcgcctgc
     1261 aaaatgtgtt caagataaaa atctatgcaa aagacggcag agtcagacga ttagatggcc
     1321 aatatcgaag ataatctttc tgcccgatag tccgttcaac cttccgcggg tgactttgac
     1381 ggtgttatgg cttcaccatt ggcctcctcg atgcattcaa atctgaacac caccttctcc
     1441 ccattttgaa tcttttcgca aaacatggct accggtctta cccaaaggcc ataagttccg
     1501 tagagcgcac gatagaccac gaggtcttct tcagtttcgg agtgtttggc gacgccaatg
     1561 acttggtatt ccttcccttt atagtgacga tatctgcccg gtttaatcat agggatatgt
     1621 tccacgtaac ctaaggttta gatatgttca ggatgatcga caatcacctt atgatagtcc
     1681 ggcctcacgt tcctgtgaag taaatggggt aaaatttttt tggcgatacg caaagagggc
     1741 tatggtccct gccaccgtgg catttaatgg aatagatgga tgaatttgtg aaatacataa
     1801 tcgttctgca aaacgaaagc acggaactcc caccccttcc tctccaataa gaagacggac
     1861 gttttttggc catttcaccg tagaaatatt cttcccctgt aaatccaggg ccgttatcca
     1921 ctttaatgtt tcggccgtat tcaggttggc aatggaacct ccccatttta aagactgaac
     1981 aaaaaccgct ccactacttg ttcgaatgac cttaggatga aagggatgaa cggcttcctg
     2041 aagaagaata accgtttgca catcgaaagc ccggcaagaa cgaatgaagg cacctaaatt
     2101 tccagggtcc cccattggac acaatacttc caaaccattg ggagcctggg tcagatccat
     2161 agaagaaatt tcagggatgt tgcagaccac gagaggttct ttggttccaa atacatccaa
     2221 ttccttaaat aaagaatgtg aaagtatata ggatttggtc tggcagggaa gatcttcttc
     2281 gatgttccat gaggatggaa acaggaactc tcgagcagaa tggccggaat tcttccaaat
     2341 ttcatttctg agtttgttgc ctgaaaccag gcattggtga tggcgtttaa ttccatgagc
     2401 gtccagtaat gataaccatc gtttaaaatg tgaatttttc cggctggaaa tagaatgtgg
     2461 ttgattcaca aaaatcctcc gggacatatc atagtgaaag acctgacaat gccacaatgc
     2521 aaccgtgtgg gaatttcatg ttcgaggcgg taacagggag cataccaaaa gtgatttaag
     2581 agacctataa gaaataccga taactgaacg aatccttccg tccccatgca ttcgtaagtt
     2641 tttattggag agtctttaga tgtcaccggt gagtaaaaga ggaggaagtc atgttgggaa
     2701 cttggtccat cagggaaaaa gatcaaaatc agaatgatgg gtcagaaaaa aatcctgcag
     2761 ccaacgtatc atctgccaca ggggcgtcaa gtgaatatga ccatgcatta gaagcattgg
     2821 ccagaattct gagaagtctt ggccgtcatg catttgaatt ggatagactc tccgaggagg
     2881 ctattgaaaa agaatttgaa ggatgggcca tgcacgtgtt ggtgggtgct cggattcctg
     2941 gtgaagaaga ggaaaaactc gagccttcga ctcggcgtga gtggggctcg ctgagtcggt
     3001 ttgtgaatgg gcatcgtcaa caggaaaaaa cctatgtcac acgaaatctg caagatatgc
     3061 gaaatgttat ttgggagttt acccatatag ttggacgcgc aattgtcgag gatcaggagg
     3121 ttgacgaaaa agtgatcaat catcttgctc aattacgttc gacgacggaa ggcggttcct
     3181 ttcaggatgt caagcaggcc ttgtgtgcag tggtacaagg catttctacg ttgatggatg
     3241 aacgaaaaaa tcgccagcag caacgactga aaagactggg agaaaaatta catgctgttg
     3301 aagcggaact tggaagtaca cgaaaacaac tgaccctcga ctctctgact caactctata
     3361 accggggggc tttggatctt caactggaac gaacggcaag cctgagcttt ttttccggta
     3421 cgccggcttg tattttaatg gtggatgtcg atcattttaa gcacattaat gatacttatg
     3481 gccatccggc gggggatgtg gtgctacaac atctggctaa acgcctggtg gcgacattcc
     3541 ccagaaagac agattttatc gcccgatatg ggggggaaga gttttgtgtc ttgcttcaag
     3601 gagctgggct tgacctgagt caacgattag gagagcggtt actggatgcc gttcgaagag
     3661 agaaatttca ttatcaagat atagaaatct cggccacggt gtcgataggt gtagctgagt
     3721 tacggccagg tgaagccgtg ccctcctgga ttgaacgtac ggatcgtgct ttatatcgag
     3781 caaaagaagg aggtcggaat caattgtgcc gggctctgga gacgaaagga gtggggcagg
     3841 aaattaaatg agaaagccgc atggggtggc cctatgcttg attaaggaag cttggaagtg
     3901 cccaactgct ttaatgccaa tcgatgaatg tggtttattt cttcaaaatt cccgaggcgg
     3961 gtgtgaaaga aactggcaat ttgaggaatt ccggtcatga tgtggctgag gaccggttcc
     4021 caatcgtgtg gttgtaaggt taaccgacct tggcttttct caccggccag ggcgatggcg
     4081 gcgtcggtat ccggattggt ccatatgtcc gcgatgatac cggaaagcgc aacagcgtcc
     4141 accagagatt gaaaatctgc aggcgtttca aataactctg gagtgtgact atacaggata
     4201 gcaatttgaa actcctcagg cagctgccaa tgctgggcaa gccaggctcc gacttgctgg
     4261 tggtcgactc ccaaatgttc aagttcaagt tgttgtaaag cggcatgatt agactgatat
     4321 tgactatgaa aaatggttaa ttcttttgag gccacctcat ttaatgccaa tatcccgata
     4381 tcttgaagaa gagcacccag aaacataagt tctgtatccg gataggcaaa atgttttcca
     4441 atggcccgac ctgcgacact tgctaagatc gatcgccgcc agaacttcac atggtccatc
     4501 ccggaagtcg atatctcttt catatctttg attaaacgat aaaaacaaaa ggaaagagcc
     4561 agatttgcga tcgttgaaaa tccaagtagg gtcacggcct gcataatagt cgtgactttg
     4621 tggcgcgagc ctccgtaata gccggaattt gccataaata aaatttgagc tgccatccca
     4681 ggatcctggc tgatgatatg tgccaactct gaagtttgag tcttttcatt ttggcacatt
     4741 ttgaggattc gagaagccag cggggataag ataggcaaag atttacaacg tttgattctc
     4801 tcaaagatgg tcgaaatttc ttcgtcctga gccatatcta atgggagtgg ttgttgcatg
     4861 aaagaatcct aaggtagttg aaggtttaga gatatgatgg ccaaattctg ccctacagct
     4921 attttttctg tctttccttt tttcggttaa aaggagtcag ggcttaaggg agtcatatgc
     4981 aaaattcata gagagaaaac ggcaataggc cgtcaatatt ccgtttttca gtcgtgcttt
     5041 ctgaacggaa g
//
LOCUS       DATIAL010000163         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_889431, whole genome shotgun sequence.
ACCESSION   DATIAL010000163 DATIAL010000000
VERSION     DATIAL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889431"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VLA60_05090"
     CDS             <1..782
                     /locus_tag="VLA60_05090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSF08766.1"
                     /translation="QEEIQEGTLIVDLEGETVGQVNGLSIHQLGDYVFGRPCRITART
                     FVGNEGVIDIQREAELAGEIHSKGVMTLAGFLGGKFAEFQPFALSATLTFEQTYSEVE
                     GDSAAVAELVAIISSLADVPVHQSLAVTGSVNQLGEIQPIGGVNEKIEGFFESCKKRG
                     LTGKHGVLIPAKNIRHLALHHEVVDAAEAGQFTVYGVHTIEEVLELLTGMPAGEIQPE
                     GQYPPNTIYGRAAQRLSEMAHIVAEWSDRRSLETVHGSKPL"
     gene            1229..2386
                     /locus_tag="VLA60_05095"
     CDS             1229..2386
                     /locus_tag="VLA60_05095"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HSF08767.1"
                     /translation="MPANQPKRDYYEVLAVARSASRDQIKQAYRQLALKYHPDRNHAP
                     DAEEHFREIAEAYAVLSDDVKRREYDTTGHVGVSERWSAEDLMRDFQFGDFFGGRFDD
                     LSTIFGDVLGRRTRPGPRMKRGLDLRYDLDLALEEAAKGGERDIHITRSEKCGTCQAT
                     GAKPGTTPTTCTECGGSGQKQQVKAGKGVRMVTLTTCAKCQGQGHWIESPCEVCHGTG
                     HVFTPHTLKVQIPQGIDDGMIIRLAGQGEPSATGGSSGDLLIRPHLRAHPTLQRQGSN
                     LFTTTSLSFVDAALGTNVPIQGLGGASLKVVVPPGTQSGTSLRIHGQGMPRLDASGKG
                     DLFVIVEVRTPTDITPQQRTLLEEFARLEKSKTLQQQDTHHMRPRQADPEK"
     gene            complement(2747..>3019)
                     /locus_tag="VLA60_05100"
     CDS             complement(2747..>3019)
                     /locus_tag="VLA60_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08768.1"
                     /translation="WTTERDWRLIAVNLSPMVAQAYIRLNGAPGMESHTSPDYTLYDQ
                     FSDLSFEGRREDLIQSGLYVRLDPFGCHIFSMGDGAVEDGKSENKI"
BASE COUNT          797 a          800 c          806 g          616 t
ORIGIN      
        1 tccaggagga aatccaggaa ggcacgctca tagtcgacct tgagggcgag acggttggac
       61 aggtcaatgg cttgtccatc catcaactgg gagactatgt tttcggacgc ccctgccgga
      121 ttaccgcccg gacgttcgta ggaaatgaag gcgtcatcga tatccaacgt gaagccgaac
      181 tggccgggga aatccacagc aagggggtca tgaccctcgc ggggtttctt ggtggaaaat
      241 tcgccgagtt ccagccattc gccttaagtg cgaccctcac attcgagcaa acctattcgg
      301 aagtggaggg agacagcgct gccgttgccg aattagtcgc cattatatcg agcctagcag
      361 atgttcctgt gcaccaatcc ctcgcggtca ccggatcggt gaatcaactg ggagaaattc
      421 aacccatcgg gggcgtgaac gaaaagatcg aaggattttt tgaatcatgc aaaaaacgcg
      481 gattgaccgg aaaacacggg gtgctgattc ccgcaaagaa catccgccat ttggccctgc
      541 atcatgaggt ggtggatgca gcggaggcag gacaatttac cgtatatggg gtccatacca
      601 ttgaagaagt gctcgagtta ctcacgggaa tgcctgcggg agaaattcag ccggagggac
      661 agtatcctcc caacaccatt tatggacgcg ctgctcaacg cttaagtgaa atggctcaca
      721 ttgtcgcaga gtggagcgat cgccgttctc tggaaaccgt ccacggttcc aagcctctct
      781 gacccaggag gggtaaatga gtctagatga acaaaaatgt tcgaacaacc acaatgaact
      841 tctcccgatt gtgcggagaa cccaagaagg aggatcgtca tgcctctgta tgattacaaa
      901 tgtctcaaat gtggcaagga atcccttcta gccctcacgc tgaaagaaca tgaaaccggg
      961 gccgcaacat gtccctcatg tggaagcaaa gaactagagc aattgatcac aagctttatt
     1021 gcaagaacca gttcgaaaag ttgacgaagg ctttcggtcg cgtgactgat aaaaaggagc
     1081 cccaacgtgt tcgcgggtgc attttccctg aggggtagca ccttttcgga gaaagggctg
     1141 cttcttgagg aagaggtctg tccctgagag aatgactctc acctcgtcca tttcattcaa
     1201 aacgttcctt ccattcgcaa aaataatcat gccagccaat cagccaaaac gtgactacta
     1261 tgaagttctg gcggtagccc ggtctgccag tcgcgaccag atcaaacaag cctaccggca
     1321 actcgcgctc aaatatcatc ccgaccgaaa ccacgcgccg gatgcggaag aacatttcag
     1381 agaaatcgcc gaagcctatg cagtgttgtc ggacgatgtg aaacgccggg aatacgacac
     1441 caccggccac gtgggggtca gtgagcgttg gtctgcggaa gatctgatgc gggattttca
     1501 gtttggagat tttttcggtg gccgcttcga cgatctgtca actatattcg gggacgtctt
     1561 aggccgacgg acacggccgg ggccgcgcat gaagcgtggt ctggatctgc gatacgattt
     1621 ggatctcgcg cttgaggaag cagccaaggg cggcgaacgg gacattcaca ttacccgatc
     1681 agagaaatgc ggaacctgtc aggccacggg agccaagccc ggcaccacgc ccaccacatg
     1741 cacggaatgt ggaggatccg ggcagaaaca acaggtcaaa gccggaaagg gagtacgcat
     1801 ggttaccctc accacctgtg ccaaatgcca ggggcaaggc cattggattg agtcaccgtg
     1861 tgaggtctgt catgggaccg gccacgtgtt tacccctcac acgcttaagg ttcagatccc
     1921 tcagggcatt gatgacggta tgatcatccg gttggccggg caaggagaac cctcagcaac
     1981 aggcggttca tccggcgacc ttcttatccg gccacacctt cgggcccatc ccactctcca
     2041 gcgacagggg agtaatttat tcaccacaac ctcgttatcc tttgtcgatg ccgcactggg
     2101 aaccaacgtg cccattcagg gattaggagg agcctcgcta aaggtggtgg ttccgccagg
     2161 aacccaaagt ggaacgtctt tgagaattca cggccaaggc atgccccggc ttgatgcctc
     2221 aggcaaaggg gacctgtttg tcatcgtgga ggttcgcaca cccacagaca tcacacccca
     2281 acaacgcacg ttgctagaag aattcgcccg gttggaaaag agcaaaactc ttcaacagca
     2341 agacacccac cacatgagac cccgtcaggc agatccggag aaatgacgca atgacaaatg
     2401 acccgcccta cggatggatt cccgccgact acctgaagga gagatgaaaa acgtctggca
     2461 tgccagacgt ttttcatcgg gcatccatct tcagaggaat gaagcatcat aggtgaggaa
     2521 attttctgac ggatccccgc taaccaccgg cgggggtaac aacacacaag gatggattcc
     2581 tgcgactaaa cggctgaggt cacggaaaag gcaacaccaa ttgttattaa ccaaggagag
     2641 gaaattctcc gacagagact gcaggagtga cggcgcaaaa attgttcgcc taagaacttg
     2701 ctgcggaagg acacctaaat cgattcacct tcatataagc tgaacttcat atcttatttt
     2761 cactttttcc atcctcaacg gctccatctc ccatagaaaa gatatgacat ccgaaaggat
     2821 cgagacggac gtacaatccg ctttggatga gatcctctcg cctgccctca aaggatagat
     2881 cactgaactg gtcatacaag gtgtagtcgg gggaggtgtg cgattccatt ccaggcgctc
     2941 cgttcaggcg aatatacgct tgggcaacca tgggactcag gttcaccgcg atgagtctcc
     3001 aatcccgctc agttgtcca
//
LOCUS       DATIAL010000164         4277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_782937, whole genome shotgun sequence.
ACCESSION   DATIAL010000164 DATIAL010000000
VERSION     DATIAL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782937"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VLA60_05105"
     CDS             <1..275
                     /locus_tag="VLA60_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08769.1"
                     /translation="FWGPIELRVQSEQRNGVRRFWRAIMAMGLTALSIFCLLTGIGTW
                     LVGSPAPGWMPWRGAWIAGLLLLGILLIPIWITVGFRQSDSVTQPQ"
     gene            complement(867..1151)
                     /locus_tag="VLA60_05110"
     CDS             complement(867..1151)
                     /locus_tag="VLA60_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08770.1"
                     /translation="MKSGILLTFLGLILFPLFALAQGGENMEPKCQKLAQEFSEDPSS
                     LNEMQLRQLQFCVTQTIERRYKTDPPDLLRGTIIEPPVSTENPDMKTPTP"
     gene            complement(1286..1372)
                     /locus_tag="VLA60_05115"
     tRNA            complement(1286..1372)
                     /locus_tag="VLA60_05115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1336..1338),aa:Leu,seq:cag)
     gene            complement(1518..3089)
                     /locus_tag="VLA60_05120"
     CDS             complement(1518..3089)
                     /locus_tag="VLA60_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08771.1"
                     /translation="MSRPLYQGTPGTLSTESIIFMKLYDLLTLASTHLNSEREVAMSC
                     GLDAPLEIAQARHLRTVGSLHLYSCDVPPGSKLLPDIPVTLLPGNDMEPTEGYLLGWQ
                     NGSLILQTFDHVGQTLNRCTLIPDTSGFLETGATRLADMASKPDSFTLGPAERLVPWL
                     DPEPLSSQTAARDSIPTTVLTTIWGDTRQNRWTKLTTLVVDQVRKNKRILLVAPDHHT
                     VDELTGAVAHALKMAALPYRSLLCRYELPLIKDAGGMLLQEFGFEAQMSHFFAKANAK
                     KVSLRKKYDRFRELTPILAYKRDKQKDLNEVKLLEWRLLTQLSDWQGKVKNIDKTLRD
                     YEALPIWTRMSMQVAGKNIETLVEYREIYQGKILGLMKEVEVAQKRIKELIPEATVPK
                     NLRPEYDELKEEIKRLGGTKKIREMLAAGEGTNRQAFAQNKRIMTCTPGRILTDPLFR
                     KVPFDLLVIDDAPRIPASLLLAATGTIRERIILSGDTSDLLPGEPGTTSSPGRWPQAF
                     LETLLSPSSQPTSVG"
     gene            complement(3035..3544)
                     /locus_tag="VLA60_05125"
     CDS             complement(3035..3544)
                     /locus_tag="VLA60_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08772.1"
                     /translation="MNHLNPRQFQTNTLFNLLLLTLFALSLFAFPQLTIAKEGMKDEE
                     GLALQPFSDLNLRFSNRPLTPPDSLIVQTIPLTGLSVARPFVAPNPQAVIFEDDHRQR
                     VAVLSTDSFGALILYLLEPLPLDPKLPALFECAHNRGCEADRTPLTGGLGCLALCIKE
                     LLELSALNQ"
     gene            3884..>4277
                     /gene="rplI"
                     /locus_tag="VLA60_05130"
     CDS             3884..>4277
                     /gene="rplI"
                     /locus_tag="VLA60_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HSF08773.1"
                     /translation="MKVILQENVEGLGYLGDVLTVAKGYARNYLLPRKKAVVAEERQV
                     KLLQHIKRQIDQKAKKELESLGESGKNLSKISLTFEVQTGKDDKLFGSVTSKDVAERL
                     AAQGVEVDRRKIHLPQPLKELGTFLVAIK"
BASE COUNT         1022 a          886 c         1190 g         1179 t
ORIGIN      
        1 gattttgggg acccatcgaa ctgcgggttc aatctgagca gcggaatggg gttcggcgat
       61 tttggcgcgc gattatggcc atggggctta cggcattgtc catcttctgt ttgctcaccg
      121 gtatcggaac ctggctggtg ggaagtcctg cccccggctg gatgccctgg cgtggggcgt
      181 ggattgccgg actcttgctg ctgggaatcc tcttaattcc catttggatc accgtcggtt
      241 ttcgtcaatc tgactctgtg acgcagcccc aataaagaat gcagaggagc aatccatggt
      301 tgtggctgcg aatgctatga aataaagagc gtagtccgta ttccggtgag ttgcagatgg
      361 agaacaactc gtgttctgaa ggaatagcta tgccggttga gattgcagag ggtttgagca
      421 tccgcccaca ccatcccatc tggaagtccc tttaaccaat ctccggtctc atttgctgga
      481 cgaatattct ctgccaattt ttttgaggat aatttcttcg ccattcaccc aggtttgaat
      541 cgtatccatt tcatctatca catcaccgcc tcccggttta atatttgtta tctgccagat
      601 aactaaggca gagggatgcg ctccattcct caaaagtcgt gtcaaatatt tctacatggc
      661 ttatgtctaa ccgggtgaat aacggggcct atctggaaag gatgtccagc gacgttaaat
      721 gatttaatgc aacggacagg aggaaaggga gggatgtcgt ggttgagagc taaatcggac
      781 ttttgccctt tgaaacgtgg ggaatttctg atgttctctt tgatggggag ggagtgcctt
      841 ttttattgtt ggccctctgg atgtttttat ggagtgggag ttttcatgtc tgggttttct
      901 gtagaaacgg gaggctcaat aatagtccct ctgagtagat ccggaggatc ggttttgtag
      961 cgccgttcga tggtttgagt tacacagaat tgtagttgcc tgagttgcat ctcattgagg
     1021 gaggatggat cctctgaaaa ttcctgggct aatttttggc acttcggctc catgttttca
     1081 ccaccctggg caagggcaaa taaagggaaa agaattagcc cgaggaatgt taaaaggatt
     1141 cctgatttca tgctgcccct cctatatata aaggaattct tttctcccgc aattctcata
     1201 ctccctgagt ttacacatat caagcaacgt attgatgaag aattactctt aggagacccg
     1261 aaaaaaaaac aaaagatata agggatggtg ccgaaggcgg gatttgaacc cgcacacccc
     1321 ggagggcgct agaccctgaa tctagtgcgt ctgccagttc cgccacttcg gcacgtgagt
     1381 gcgattatca tcttttatag aaatgcggtc aagttgtcgt tatttcgttg ttgaggttct
     1441 ctcgaagaaa tcccacctga gccattcttc ctgaatgaga cctcagcgtt acaagaaatg
     1501 acggactttt cgtaaagtta tcccacgctg gtaggttgtg aagaaggaga cagaagggtt
     1561 tccaaaaatg cttgaggcca gcggccagga gaagatgttg tccccggttc tccaggtagc
     1621 aggtctgagg tgtcaccact gagaatgatt cgctcacgaa tggtgccggt ggcagcaagc
     1681 aagagagatg cagggatgcg tggggcgtca tcaataacca gtaaatcaaa cggaactttt
     1741 cggaagagcg ggtcggtaag gattcggccc ggggtgcagg tcatgatacg tttgttttgt
     1801 gcgaaggctt ggcggttggt gccttctccg gcggccagca tttcccgaat tttttttgtg
     1861 ccgcccaatc gtttgatttc ttcttttaat tcatcgtatt ccggtcgcag gtttttggga
     1921 acggtcgctt cggggatcaa ctctttaatt cgcttttgtg ctacttcgac ttctttcatg
     1981 agtccaagga ttttgccttg gtaaatttcc cggtattcga ccagggtctc gatgtttttg
     2041 ccggccacct gcatactcat ccgtgtccaa atgggaagtg cctcatagtc ccggagagtt
     2101 ttatcgatat ttttgacttt cccctgccag tcactgagct gtgtgagtaa tcgccattcc
     2161 aacaatttta cttcgttcag atctttttgc ttgtcgcgtt tgtaagccag aattggagtt
     2221 agttccctga accggtcata tttttttcgt aatgagactt ttttggcgtt ggccttagca
     2281 aaaaaatgag acatctgtgc ttcaaagcca aattcctgga gaagcatccc tccggcatct
     2341 tttattaaag gtagttcata ccggcagagc aaacttcggt agggcaaggc cgccattttt
     2401 aaggcgtgtg cgaccgcacc agttaattca tcaacggtgt gatgatccgg agccaccaag
     2461 aggattcgct tgtttttccg aacttgatcg acgactaatg tcgtcagctt tgtccagcgg
     2521 ttttgccgcg tgtctcccca gatagtcgtc agcactgtgg tcgggatgct gtctcttgct
     2581 gccgtttgac tggagagcgg ttcagggtcc agccagggca ctaatcgttc ggcaggccca
     2641 agtgtaaagg aatcaggttt gctggccatg tcggccaacc gtgtggcccc ggtttcgagg
     2701 aaacccgatg tgtcgggaat gagggtgcac cgattgaggg tttggccgac gtgatcgaat
     2761 gtttggagta tgagagagcc attttgccat cccaggagat agccttcggt cggttccata
     2821 tcgttgcccg gtaacaatgt cactgggatg tcgggtagga gtttacttcc cgggggaaca
     2881 tcacaactat agaggtgaag ggagccgact gtgcggaggt gacgggcttg ggcaatctcc
     2941 aaaggagcgt ctaatccaca gctcatggcg acttcccgct cgctgttgag atgggttgag
     3001 gctaaagtga gaagatcgta gagtttcatg aaaattattg attcagtgct gagagttcca
     3061 ggagttcctt gatacagagg gcgagacatc ccagtcctcc ggtgagtgga gttcgatcag
     3121 cctcacaccc tcgattgtgt gcacattcaa acagggcggg tagcttagga tcaagaggga
     3181 gaggctcaag taaatagagt atgagcgccc cgaagctatc cgtcgagagg acggcgaccc
     3241 gttgtcggtg atcatcctca aaaatcaccg cttgtgggtt cggagcgaca aaggggcggg
     3301 cgacggagag tccggtgagg gggatggttt ggacaatgag tgagtcaggt ggggtgagag
     3361 gacgattgga aaagcgaagg ttgaggtccg agaagggttg gagtgcgagt ccttcttcgt
     3421 ccttcatccc ttccttggca atggttagtt gagggaaggc gaaaagacta agggcaaata
     3481 gggtgagaag gagaagattg aaaagtgtgt tggtttgaaa ctgacggggg ttgaggtggt
     3541 tcataatctc actattccga cacagagagt atggtagatg aaaaagcaat ctgatcgttt
     3601 atcttggctg taatttatcg atggcctcta ggtttgaggt tcggcagcat tttaatactt
     3661 aagtgttaat gtttagtaag tatggaggag catagcatat ttttgaaagg gttgttttga
     3721 aattctctat ggaattatct ggttttcata agcatttctg taggttgacc accgattatg
     3781 aaggagaagc atattctatg atatattcag caattacatt gactttttct atacccagtc
     3841 ttaagatgtt tcccttattc gctaggctaa ggagacgtgt tttatgaagg ttattttgca
     3901 ggaaaatgta gaaggtttag ggtatttggg agatgtatta acggtggcga aagggtatgc
     3961 acggaactat ttactcccga ggaaaaaagc tgtcgtagct gaagagcgac aggtcaagct
     4021 gttgcagcat atcaagcggc agatcgacca aaaggccaaa aaagaattgg agtctttagg
     4081 cgagtcgggg aagaatctgt caaaaatctc cctgacgttt gaagtgcaga ccggaaagga
     4141 cgacaagttg ttcgggtctg tgacatccaa agatgtggcc gaacgattgg ctgctcaagg
     4201 cgtggaggtg gaccgaagga aaattcactt gccacagcct ctcaaggagt tggggacctt
     4261 tttggttgct atcaagc
//
LOCUS       DATIAL010000165         5413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_460189, whole genome shotgun sequence.
ACCESSION   DATIAL010000165 DATIAL010000000
VERSION     DATIAL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460189"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VLA60_05135"
     CDS             <1..778
                     /locus_tag="VLA60_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF08774.1"
                     /translation="VAGALALFLADQENLSWNAVYFIMGGMMIIGILTTWFAPEPTIP
                     APPPASWQEAITGPFLEFFSRPGALMMLLFILLYKVGDSMASEMLSPFMVDLGVSKTD
                     YAMIVKVFGMIALMAGGLIGGLVVYRLGIVPSLFILGFLQMISTAGFVILAYTGNHLP
                     TLTAVIAFETISSGLGQTAFVAFMASLTNKRFTATQYALLTSFMGIPRVFAGSTTGIL
                     ATWLGWEGFFLLCTLIALPGLFLIPYLRRLEENHQSITRL"
     gene            complement(889..1308)
                     /locus_tag="VLA60_05140"
     CDS             complement(889..1308)
                     /locus_tag="VLA60_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08775.1"
                     /translation="MVLVFGKTGILGVLLLLSLAGCDFRRVVVNDPLVADSLEELVPG
                     KSTIQDVAGKLGAPDEIEEGAGGMVFRYRYGDSKTMRVNFGWLLRVLLPVSPSMNLGR
                     GEGVTYILHVALRPDLTFDHSVIQPPPDTPQFWFWPF"
     gene            complement(1315..1734)
                     /locus_tag="VLA60_05145"
     CDS             complement(1315..1734)
                     /locus_tag="VLA60_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08776.1"
                     /translation="MLRESQAGFSHHWRNPRGLLLFAGLLVLLSMVNGCAFVRGEVGE
                     PFPEDRIETIEKGITNRQEVAQQFGAPDEIVQANGYEIFHYRRFDSKLGWLLFLSRLN
                     IGSDHLWVFFNSQGIVDDVVFGNRTRNVEFQIWPFGE"
     gene            complement(1749..2540)
                     /locus_tag="VLA60_05150"
     CDS             complement(1749..2540)
                     /locus_tag="VLA60_05150"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HSF08777.1"
                     /translation="MLTLSHVSKIIGLLLVLFVALWLVHLRVGNASFADVGFCLGFGL
                     VVAICGIEGEGSPWRRALVGGMGSAYACRLGWHLWKNRIWGKSEDPRYKTVRAVLGTW
                     ESVGIFGYFMLQVPACLFFGFLLCWIMGHSESAVRGWDLLGLGIFLGAFFGEALADIQ
                     LERFRSDPTNQGKVLQTGLWRFSRHPNYFFEILQWCAYIPLAVGLPGAWMAIMWPLLM
                     MASLLWVTGVPLAEAQAMNSRGEAYRRYQRTTNKLFPWPPRRHSS"
     gene            complement(2619..>5413)
                     /locus_tag="VLA60_05155"
     CDS             complement(2619..>5413)
                     /locus_tag="VLA60_05155"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HSF08778.1"
                     /translation="LFTLQINGFDVQEPDGSPLFGFGELFINFEPAASLVKRAYTFAE
                     IRLELPYGLAWVRADGSLNLAALGSSSESQDVPPEALSEESSSPESAELPAILIEQLS
                     IQRGMLEFRDYSRPTPFVAHFVPINLTLEQFSTQKGQANSYALSAERSAGEKITWEGT
                     VTLEPLQSEGRLVFEDYQLPRLWTYVQDLVQFEIPQGQITMKGDYRLFSTDQGVNVLV
                     DEGTLTVHDLQIQEKGTLSPVITIPLFEVNGVSVDVAKQDVQIPSIKSRDAHFTGWVG
                     KDGVVNYQTLFSPVALESQTAPEPETAPANPWNMVIEDIALDNFTIDFEDRQPEDPVK
                     LLLDTIHFHTSNVSLDLDRPLPLDLSLQFNQTGKANLQGVLKIDPLSIELDLSLKNIA
                     LQPFQPYMAPFVQFEVGDGALTLQGSTHYQAEATTQPMVTFKGGIGISHFALEDPIQD
                     NSFLTWEAFELKALDLQIEPTIVNLQKIELINPALALLIDEEGRLNLQRLFSPPGSVS
                     QEETASDETPLEAESPGSSPTPVKIESVSLTNLLARFTDQSVSPNVVTKIDGLSGTIK
                     GLSSDQLAKADVALQGTVDKYAPFTIAGQINPLSEEAYTDVTVTFNNLDLPTVSPYSA
                     HYVGYPITKGKLSLDLEYKVSEKTLVGENKVLIDQLTMGEKIESPDATSLPIPLAVAL
                     LKDRKGQIDIDLPVRGDLDDPDFSYGGVIWNALGNLLTKVATSPFAMVGGLVGGSGDD
                     LQYVAFPAGIAQLSPAEQEKLHALGQALGDRPALRLDIAGAADPQVDRQGLAAGELRK
                     QLQQRKFVQGSSSAREGMSLEQIELSPEEEGRLLAEVYAEQFGAQPKTPASPPGGKTP
                     DIPSPEQMKSKLLESIKVEDEQLRLLAQQRAQGIREFLIQEGKVSGDRVFLLEPNLSP
                     VTEEQTVRSPLALAAN"
BASE COUNT         1395 a         1476 c         1282 g         1260 t
ORIGIN      
        1 ggtcgctgga gcgttggctt tatttctggc agatcaggag aacctttcct ggaatgctgt
       61 ctatttcatc atgggcggca tgatgattat tggcattctc acgacatggt ttgcaccgga
      121 acccaccatt cccgcaccac cacccgcatc atggcaggag gccatcacag gtccgtttct
      181 ggagtttttt tcccgtccgg gcgctctcat gatgctgttg ttcatcctgc tgtataaggt
      241 cggcgatagt atggcgtcag aaatgctctc tccatttatg gtggatctcg gcgtctcgaa
      301 aaccgactat gcgatgatcg tcaaggtttt cggcatgatt gccttgatgg cgggtggatt
      361 gattggtggg ctggtggtct accgcctggg gattgtgcca tccctgttta ttctgggttt
      421 tctccaaatg atttcaacgg caggatttgt cattctggcc tataccggca atcacctccc
      481 gaccctgacc gccgtcattg cctttgaaac catttccagt ggactgggac aaaccgcgtt
      541 cgtggccttt atggccagtc tcaccaacaa gcggtttact gccacacaat atgccctgtt
      601 gaccagcttc atgggcattc cacgggtctt tgccggctcc accaccggaa tcctggccac
      661 ctggttggga tgggaagggt tttttcttct gtgtacctta attgccctgc ccggcttatt
      721 cctcattccc tatctccggc gcttggaaga aaatcaccaa tccatcacca ggctataggc
      781 cttgtcgagc cagccatggt ggagttgaga cacgcaaggg cgaccacatc ccctatttcc
      841 aaatgaagtt gaaacatttc ctaatggtta ccttgcacct taaggcagtt aaaacggcca
      901 aaaccagaat tggggggtat cgggtggggg ctgaatgact gaatgatcaa aggtcaaatc
      961 cggtcgcaac gccacatgta aaatataggt gaccccttca cctcttccca ggttcatgga
     1021 tggagaaacg ggcaacaata cacggagaag ccatccgaaa tttacccgca tcgtcttgct
     1081 atctccatat cgataacgaa aaaccattcc gccggcccct tcctcaatct catcaggagc
     1141 gcctaatttc ccggccacat cctgaatcgt gcttttccct ggcaccaact cttcgagaga
     1201 gtcggcgact aaaggatcat tgaccaccac acgacgaaag tcacagccgg ctagggaaag
     1261 aagcaacagg acaccaagga ttccggtttt tccaaagacc aagaccatct tgccctactc
     1321 cccaaacggc cagatttgaa attccacatt cctggtccga ttgccaaaca ccacgtcatc
     1381 cactatccct tgggaattaa aaaataccca gagatgatca ctgccaatat taagacggga
     1441 cagaaacagc aaccatccca acttgctgtc aaatcggcgg tagtgaaaga tctcatagcc
     1501 gtttgcctgg acaatctcat caggggcccc aaattgttga gcaacttcct ggcgatttgt
     1561 tatgcctttc tcaatcgttt caatccgatc ctccggaaaa ggctcaccca cttctccccg
     1621 gacaaatgca cagccattca ccatgctcaa caacaccaga aggccggcaa aaagtaggag
     1681 tcctcttgga tttctccaat gatggctgaa gccggcttga gattcccgca acatcgttct
     1741 cctcctcttt atgagctgtg ccgtcgaggc ggccagggaa acaatttatt ggtggtgcgt
     1801 tgatatcggc gataggcttc ccctcggctg ttcatagcct gagcctcggc caagggcact
     1861 cccgtcaccc ataagagcga cgccatcatg agcaaaggcc acatgatcgc catccatgcc
     1921 cccggcagtc caactgccaa cggaatatag gcacaccact gtaggatttc aaaaaaataa
     1981 ttaggatgac gggaaaaccg ccacaacccc gtctgtaaga ccttgccctg attagtcgga
     2041 tcggatcgga accgttcaag ttgaatatcc gcaagagcct ctccaaagaa ggctcccaaa
     2101 aagatcccga ggcccagaag gtcccagcct cttaccgccg attcagaatg gcccataatc
     2161 cagcacagga gaaatccaaa gaacagacaa gcaggaactt gcagcatgaa gtacccgaag
     2221 attccgaccg attcccatgt gcccagcacc gcccgaacgg ttttataccg ggggtcttcc
     2281 gactttcccc aaatgcgatt cttccatagg tgccagccca accggcaggc atacgcactc
     2341 cccatgcccc caacaagagc ccgtcgccaa ggactgccct cgccctctat tccgcaaata
     2401 gcaacgacaa ggccaaatcc taagcaaaat cctacatcgg cgaaggaggc attgcccact
     2461 cgcaaatgta caagccacaa ggcaacgaag agcaccagga ggagacctat aatcttagac
     2521 acatgcgaaa gagtgagcat atgccaggaa agcggattca ccttagatca gaaaacagac
     2581 ggtcaccacc acgaccatga agatctttgg aaagggactc aattcgcggc taaggccaaa
     2641 ggactccgaa cggtttgctc ttccgtgacg ggacttaagt ttggttcgag gaggaacact
     2701 cggtcaccgg atacctttcc ttcctggatg agaaattctc gaatgccttg agcccgttgc
     2761 tgagccagga gacgaagctg ctcatcctcc accttgatcg attcaagcaa tttggatttc
     2821 atctgctccg gtgaaggaat gtccggagtt ttccctcccg gaggagaagc cggagttttg
     2881 ggttgcgctc caaattgctc ggcatacacc tctgcgagca agcgcccttc ctcctctgga
     2941 ctgagttcaa tctgctctaa agacatcccc tccctcgcgg atgaggagcc ctggacgaat
     3001 tttcgctgtt gcagttgttt ccgcaattcc ccggcagcca aaccctggcg atcgacctgg
     3061 ggatcggcag ccccggcgat atccagtcgc aaggcaggac gatcgcccaa cgcttgacca
     3121 agcgcatgca gcttttcctg ttcagcagga gacaactggg caatccccgc aggaaaggcc
     3181 acatattgca gatcatcacc gctgccaccc accaatcctc cgaccatggc aaaaggagaa
     3241 gtcgcaactt tcgtcagaag atttcccagg gcattccaga tcactcctcc ataactaaaa
     3301 tccggatcgt ccaaatcccc acgaacgggc aaatcaatat cgatctgtcc tttgcggtct
     3361 ttcagtaagg ccacggcaag aggaatcggc aacgacgtcg catccggact ttcgattttc
     3421 tcacccatgg tcaactggtc aatcaacacc ttattttcac caacgagagt cttttcagaa
     3481 actttatact caagatccaa cgacagcttc cccttcgtga tgggataccc gacataatgg
     3541 gccgaataag gagagacggt ggggagatca agattgttga acgtgacggt cacatccgta
     3601 taggcctctt cactcaaagg attgatttgc ccggcaattg tgaatggggc gtatttatct
     3661 accgtccctt gtaaagcgac atcggctttg gctaattgat ccgaagaaag gcccttgatc
     3721 gtaccgctca aaccatcaat ttttgtcacg acattcggag aaaccgattg atcggtgaaa
     3781 cgagccagaa gattggtaag agacaccgac tcaattttga ccggagtcgg agacgatccg
     3841 ggagattcgg cctctaacgg cgtttcgtct gatgctgttt cttcttgaga tacggatcct
     3901 ggcggtgaaa acaaccgttg aagatttaac cttccttcct catcaatcaa caaggcgagt
     3961 gcagggttaa tcagttcgat tttttgaaga tttactatgg ttggttcaat ctgaagatcc
     4021 aaagccttca gctcaaacgc ttcccaagtc aaaaaagagt tgtcctgtat gggatcttca
     4081 agggcaaagt gggatatccc aattcctcct ttaaaagtca ccattggttg agtcgtagct
     4141 tccgcctgat agtgtgtgct tccttgcagc gtgagcgccc catcgccgac ctcaaattga
     4201 acaaagggag ccatatacgg ttgaaacggc tgaagggcaa tgttctttaa tgacaggtca
     4261 agttcaatgg acaggggatc tatcttcaac accccttgca aattagcctt gccggtttgg
     4321 ttgaactgta acgagagatc gagaggaaga ggtcgatcca aatccaagga cacgttcgag
     4381 gtgtgaaaat gaatggtatc caatagcagt ttgacagggt cttcaggctg gcgatcctcg
     4441 aaatcaatcg tgaagttatc caaggcaata tcctcaatga ccatattcca gggattggcc
     4501 ggggcggttt ccggttcagg agctgtctga ctttctaatg ccacaggtga aaacagcgtc
     4561 tgataattga ccactccatc cttcccgacc cacccggtaa aatgagcgtc gcgagacttt
     4621 atcgaaggaa tttgaacgtc ttgcttggcc acatcaaccg agacgccgtt gacttcaaag
     4681 aggggaatcg tgatcacggg ggagagggtc cctttctcct gaatctgtag atcatgaacc
     4741 gttagggttc cttcatcgac caagacattc accccctggt cagtcgagaa caatcgataa
     4801 tctcccttca tggttatctg tccttgagga atttcaaatt gaaccagatc ctgaacatag
     4861 gtccacagtc tgggcaattg gtaatcttca aaaaccaagc gcccttcgga ctgtaagggc
     4921 tccagagtga ccgtcccttc ccaggtaatt ttctcacctg cactgcgttc agctgagagt
     4981 gcataggaat tggcctgacc tttttgcgta ctaaactgtt ccaaggtgag attgattggc
     5041 acgaaatgcg ccacaaatgg agtcgggcgg gaataatccc gaaattccaa catgcctcgt
     5101 tgaatggaaa gttgttcgat gagaatcgct ggcaattctg ctgattctgg tgatgaggat
     5161 tcctcagata gggcctcggg agggacatcc tgagattcgg aggaagagcc taatgcggcg
     5221 agattcaagg acccgtcggc gcgaacccat gccaatccat acggcagctc caatcgaatt
     5281 tccgcaaacg tgtaggctcg cttcaccagg gaagcggccg gttcaaaatt aatgaacagt
     5341 tcaccaaacc cgaataacgg agagccatcc ggttcctgaa catcaaaccc gtttatctgc
     5401 agtgtaaaca gaa
//
LOCUS       DATIAL010000166         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1320736, whole genome shotgun sequence.
ACCESSION   DATIAL010000166 DATIAL010000000
VERSION     DATIAL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320736"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VLA60_05160"
     CDS             complement(<1..1149)
                     /locus_tag="VLA60_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSF08779.1"
                     /translation="MTTLLICRLIVFCLILGLFKTIIIPQTVRAEGFRILDHGASATG
                     QGAAFTAQADDASALHYNPAGMTQLKGIQFYAGTLLIGGTVRFKSDLGPTVKGGVGGT
                     ISTPPPSSFFLTANLPALGLDNLPNWTVGIGVTTPYALNIEYPDDSLISPILTKASLP
                     LIDIKPTLAYKVNDYISVGGGLDIYTFASFLGEGQAELKQNAAPGNPFGIPAGTGLEA
                     NGSDTALGFNASVLWTPFRNQDGHPLVNLAFVYRNGPNLDLKGDFLAGGTRLAGASTN
                     IELPDIYTWAIAGWPIRNAQHEWKVEVDLDYADWSGFKDLNLQLSNGAVIQNPRNYGD
                     AWILMVGTEFKALSPSALPHWDVAFRTGYVRSESPIPNRTFEPAVPDSD"
     gene            1319..1774
                     /locus_tag="VLA60_05165"
     CDS             1319..1774
                     /locus_tag="VLA60_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF08780.1"
                     /translation="MKRDSVPQVGQLNVEKENGMRVKKLLHTRMRVSDMEQTLGFYRE
                     VLGLEVVEQKVSPRGSHLAFLAVPNSEELIELCSFPSSGTVKVQEDLVHLAFEVEDLE
                     QTIQELQAKGVPITDGPTQSSSGSRFIFIDAPDGYEIELIQRPAGVAIV"
     gene            1774..>2987
                     /gene="uvrC"
                     /locus_tag="VLA60_05170"
     CDS             1774..>2987
                     /gene="uvrC"
                     /locus_tag="VLA60_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSF08781.1"
                     /translation="MIKVAKQEMMDTPTRPSQVLSDQPGRLSGILNNLPKQPGVYLFK
                     DRRGDIIYIGKAAVLSDRVRSYFQHAADLTPKNRVLYSQIADLETIVTHSEFEALILE
                     SNLIKRHRPRFNVVLRDDKQYPYLRLPIHDDFPRLSIVRRVKQDKALYFGPYVPTGAM
                     RDTLKVIRKVFPMATCTIDINGSAERACLEFEIKRCMAPCTGNQTKEAYHQIVKQVRW
                     FLEGRDTELLESLRSAMQDAAEREAFEEAARLRDQIGNIERMLAKQRVAQKSAREQDI
                     VGLARIGGAVDVQMLFVRGGLLIGRRDFFWSDAHDVTDEELVRSTLEQFYAKTVVPPK
                     EVLLPVGFDDQSLVQRWLSEKKEEAVRVLVPERGVKYELLQLAQENAAVSLNEHLRVQ
                     TESSESVEELQSL"
BASE COUNT          739 a          684 c          873 g          691 t
ORIGIN      
        1 atcggaatct ggaaccgctg gctcgaacgt ccggttgggt atagggcttt ccgatctgac
       61 gtatcccgtt cgaaaggcca catcccaatg agggagcgca gagggggaga gggctttgaa
      121 ttccgttccc accatgagga tccaggcatc tccatagttg cggggatttt ggatgaccgc
      181 tccattagag agttgtaggt tgaggtcttt gaacccggac caatccgcat aatcgagatc
      241 cacttcgacc ttccactcat gttgcgcgtt tcgaattggc cacccggcga tggcccacgt
      301 gtagatatcg gggagttcaa tgttcgtgga ggcaccggca aggcgggttc caccggctaa
      361 aaaatcccct ttgaggtcca aattgggtcc gttccgatag acaaacgcga gattgacgag
      421 tggatgaccg tcttgattgc ggaaaggtgt ccagaggaca ctggcgttaa atcccaaggc
      481 ggtgtcagac ccgttcgctt ccaggcctgt cccagcgggt ataccaaacg gattgcctgg
      541 tgccgcattc tgtttgagct ctgcctgacc ttcgcctaga aagctggcaa aggtataaat
      601 atccagcccg cctcctacag agatatagtc attcaccttg taggcgagag tgggcttgat
      661 atcgataagg ggaagggacg ctttcgtgag tatgggagaa atcaggctat catcggggta
      721 ttcgatattc agggcatacg gggtggtaac cccaattcct acggtccaat tcgggagatt
      781 atccaggcct aaggcaggaa ggttagcagt gaggaagaat gaactgggtg ggggagtcga
      841 aattgtccca ccaacaccac ccttcacggt tggtccgagg tcgcttttga atcggaccgt
      901 gcctccgatc aagagagtcc ctgcataaaa ttggatgcct tttaactggg tcatacccgc
      961 tggattgtag tgcagggctg aggcatcatc ggcttgggcg gtaaaggcag cgccttggcc
     1021 ggtagccgaa gcgccatgat cgagaatacg aaatccttca gcacgaactg tttgaggaat
     1081 gatgatggtt ttgaagagcc caaggatgag gcaaaaaacg atcaggcggc aaatgagcaa
     1141 tgtcgtcacg tctcctcctt gagaacggga accgatcgga aattccgggg aagtctatga
     1201 aaaacaagag aagatagcaa tcttgatcag ggaggagcaa cgagtgacaa tagcgaaagt
     1261 gatgtctccc gatcggggag cacggactta ctctttattt tgttaagaag tttgattcat
     1321 gaagagggat tccgttcccc aagtcggaca gctgaatgtt gaaaaggaga atggcatgcg
     1381 ggtgaaaaaa ctgttgcata cgcgaatgcg ggtaagtgac atggagcaga cgctgggttt
     1441 ttatcgagag gtccttgggt tggaagttgt ggagcaaaag gtatcgccac gaggttcgca
     1501 tctggcattt ttggctgtcc ccaatagtga ggaattgatt gagttgtgca gtttcccgag
     1561 tagcgggacg gtcaaggttc aagaggatct tgtgcatttg gcctttgaag tggaggattt
     1621 ggaacagacg attcaggaac tccaagccaa aggtgtgccg atcacggatg gccccaccca
     1681 atcttcatcc ggcagccggt ttatttttat cgatgcgccg gatggctatg aaattgagtt
     1741 gatacaacga ccagcgggag tcgccatcgt ctgatgatca aggtggcaaa gcaagagatg
     1801 atggatacac ccacgcgccc ttctcaggtc ctctctgacc aaccaggtcg cttgagcgga
     1861 atattgaata atcttcccaa gcaaccagga gtctatcttt ttaaggatcg acgaggcgac
     1921 attatctata ttggaaaagc cgccgtgtta tcggaccgag tgcgatcgta ttttcaacat
     1981 gcggctgatc tcactcctaa aaaccgggtg ctgtattcgc agatcgcgga tttggaaacg
     2041 atcgtcaccc actcggaatt cgaagccctc attttggaaa gtaatctgat caagcggcat
     2101 cgcccacggt tcaatgtggt cctccgggac gataaacaat atccctatct ccggttaccg
     2161 attcacgatg atttcccgag gctgtcgatc gtccgccggg tcaaacagga caaggcgtta
     2221 tattttggtc cgtatgtccc cacgggagct atgcgcgaca ccctgaaagt tattcgcaag
     2281 gtgttcccca tggccacatg cacgatcgat attaatggaa gcgctgagcg ggcttgcctg
     2341 gaatttgaaa tcaagcgttg catggctcct tgcacgggaa atcagacgaa ggaagcctac
     2401 catcagattg tgaagcaggt tcgttggttt ttagagggac gggacacgga attgctcgag
     2461 tccttacgat cagccatgca ggacgcggcg gagcgggaag cgtttgaaga agccgctcga
     2521 ctgcgtgatc agattggaaa cattgagcgg atgttggcca agcaacgggt ggcacaaaaa
     2581 agcgccaggg agcaggatat cgtgggattg gccagaattg gaggggcagt ggatgtccag
     2641 atgctgtttg tgagaggcgg cctgttgatc ggccgtcggg attttttctg gtccgacgct
     2701 cacgacgtca ccgatgagga gctggtgaga tcgacgctcg aacagtttta tgccaaaaca
     2761 gtcgtgcctc ccaaagaggt tctgcttcct gtggggtttg acgatcaatc gctggtccag
     2821 cggtggttgt ccgagaaaaa agaggaagcc gttcgtgtgc tggttcctga acgaggggtt
     2881 aaatatgaat tactccagct cgcacaggaa aatgcggcag tgtctctcaa cgaacatctt
     2941 cgggtgcaaa ccgaatccag tgagtcggtg gaagaacttc agtcgct
//
LOCUS       DATIAL010000167         5344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1212873, whole genome shotgun sequence.
ACCESSION   DATIAL010000167 DATIAL010000000
VERSION     DATIAL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212873"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            991..2229
                     /locus_tag="VLA60_05175"
     CDS             991..2229
                     /locus_tag="VLA60_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSF08782.1"
                     /translation="MMFGNLLAGCLQFNPSRTYDADIVNYQASSGIVTTSLNGQTSTI
                     EANKPWQASGVTVTPGDTVKILAMGKWSQAPALNVWSGPEGLDGTGKEVPWINANALM
                     GKIGEQGKPFEIGTETELRATGEGEIYLAINDPFPWIEDNAGSMQVTVYIQKDSLPLA
                     NLQPTERTSLSSQAKPAPAIPAEAGKRTALVLGNSEYQIGSLRNPANDAQDMAEALMK
                     LRFDVTLELNADLEKMEHAISEFGRHLYQGGVGLFYYAGHGVQVGGENYLIPVNAAIE
                     SESDVRYKAVNIGQVLGKMGEARNGFNIVILDACRDNPFAKEFRSSSRGLAVVNSSFV
                     KGTLIAYATSPGKVASDGENRNGLYTQHLLQHISSPGLPVEQVFKLVLQGVERDTNGK
                     QSPWTSSSFSGNFSFQPTVQ"
     gene            2708..4087
                     /locus_tag="VLA60_05180"
     CDS             2708..4087
                     /locus_tag="VLA60_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08783.1"
                     /translation="MNMSFAKRVLCAMFVSIVFQSGLPAQAVIPDETTTITLDQAVHF
                     LGTDGSDVVAEPGNYSVEAAQEWLRLIPGTERRDALLIEAQQGTHEVKVEIPIVISTP
                     GSEPDELDVHVVQFLNPDGTSMVATGTYSGIQSRGLFDSAKKAAARAKAVAEAARRVA
                     AQKAAAARAAALRAKQAAEQAAQAAAAKAQELARQTAQFAKVQACKIQVSAIRGGLKA
                     GQAAESLMKQVAAILPVARQRQGSLQNRLNQDQAYREQIVNTMASGINANKYVIPEMN
                     RIQANLNNPANRSKFMDLLFSPTMICENSIADLDRALIQTGLAPSFAIVRTRGATENH
                     FYMGFQVTLDAAYVGGAQIGFFGVTDFRGNGGWHWFIGPEAGIVVGGGGSLEVIFFPK
                     VNLDSFAGWGAGIGGSLGAGATGNADGMWDGSVKEFQGFGVGGGGGVGSKMVGSLAAS
                     YTHAWKIGK"
     gene            4200..4706
                     /locus_tag="VLA60_05185"
     CDS             4200..4706
                     /locus_tag="VLA60_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08784.1"
                     /translation="MKDRKFSILVTALNVVILFGLFTSGLAEDANQRIQDRVSPPSTR
                     KILSPSGREPVTKALPTTDQKFTTIFSKLATLEQIVASLQNQVTAQAQLITQLKQVIS
                     VNSSGTVTIQAPGTLKIAAGGNLNLSGSTVDVNTAITGIHGILKADTMMTTTVIAKSY
                     TPGAGNIW"
     gene            complement(4890..>5344)
                     /locus_tag="VLA60_05190"
     CDS             complement(4890..>5344)
                     /locus_tag="VLA60_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF08785.1"
                     /translation="VRGSIVIVQDADLEYSPEDYHRLLEPVITGRADVVYGSRFLGGW
                     KGGWPPVSYLGNKIFTTLTNAATGLSLTDVWTGYKVFKQEVLSDISLEESGFELELEL
                     TLKVAQKNWRVTEVPISYLPRSKADGKKIRWRDGIQSIKTLYRYSHTS"
BASE COUNT         1413 a         1390 c         1267 g         1274 t
ORIGIN      
        1 aaaacaacgt ttgcatccgc atagtatcgg caaggagcct ttttatctgg attctgggag
       61 aaggcctcgc cttcccagag acccagccgg aagaactatc ggactctcct tcaactgtct
      121 agggaaacct aatcccgtcg gaggacgaag gtgaattaat tgaacgcgag atccggattc
      181 gcgattatcg cgaacttcct cgattgttgc cctggaaacc aacagcgccc agcgcacccg
      241 caccaaaagt cctcgtgact tcagttggtg gaccatccac agacggcaca ctccctcaga
      301 gcacctacgg tactgactag accccaagcc tgacgattat cgcggacggc tagacgtgtt
      361 gcaattgaag ttagaatcta cccccttgaa aagtgaattg gatcaccgac ataatttttt
      421 tactaaagag ttcgacctct aaatttttcc tatacccaca ctcaactatc gcaccaaagc
      481 aattctgcat tcgcctgacc tggcaatacc ttttacggtc ttaatttttc atcctcaaaa
      541 tttggaccgg gcgttcatga aattctctct ctcaaacacg ttctcgagag ccatgcttat
      601 ctgtgatttg ctgagtcccg ttttctccgc tcaccctcag agtttgatga caccacgacc
      661 atcacgctcg accaaaccgt gcatttcatc ggtacggagg gcaataacgt ggtcgcaaac
      721 tccagggagt atgcctttga ggcgacgtag aaatagcttc ggcttattcc cgaaaccgaa
      781 cgccggggtg aattgttgag ggattttctt tgaccttccc cacatccctc agtaaaattt
      841 cttgaagttg ccagacattc ccgcgaatgg ctggaccaga atcaggctca ccctcattta
      901 tctgtggcat tcatctgacg gagatatacg ttcaatgttt tttcaacaaa acgccaatac
      961 atttctcagt acacgacatt ctgccttgcc atgatgtttg gcaacctgct ggccggctgc
     1021 cttcagttca atccctctcg cacctacgat gccgatattg tgaactatca agcgtcctca
     1081 ggaatagtga cgacctccct gaacgggcaa accagcacca tagaggccaa taaaccctgg
     1141 caagcctctg gagtcacagt gaccccgggt gacacggtga aaattctggc tatgggcaaa
     1201 tggagtcaag ctccggcctt gaatgtttgg agcggcccag aagggttgga tggcacagga
     1261 aaggaagtcc cgtggatcaa cgccaacgcc ctcatgggca aaattggtga acagggaaaa
     1321 ccctttgaaa ttggaacaga aactgagctc cgagccacag gagaagggga aatctatttg
     1381 gccatcaatg accccttccc ctggatcgag gataatgccg gttccatgca agtcaccgtg
     1441 tatatccaga aagattctct tcccctggcc aatctacaac ccacagaaag gacatcactc
     1501 tcctctcaag caaagcccgc ccccgccatc ccagcagaag cagggaaacg tactgctctg
     1561 gtcctaggca atagcgaata ccagattggc agtcttcgga atccagccaa tgatgcgcag
     1621 gatatggctg aagcactgat gaaactcaga tttgatgtga ccctcgaatt aaatgctgac
     1681 ctagaaaaaa tggaacatgc catcagtgaa tttggcagac acctctatca gggtggagtc
     1741 ggactgtttt attacgctgg ccatggcgtg caggtcggcg gagaaaacta tctcattccc
     1801 gtcaatgccg ccattgaaag cgaaagcgat gtccgctaca aggccgtgaa tatcggccag
     1861 gttctcggca aaatggggga agcccgaaat ggattcaata ttgttatcct ggatgcctgt
     1921 cgagacaacc cctttgcgaa ggaatttcgt tcatccagtc gtgggcttgc agtcgtcaat
     1981 agctcattcg tgaagggaac gttaatagcc tatgccacaa gcccgggaaa agttgcgagt
     2041 gacggagaaa atcgaaacgg cctctatact caacatttac ttcaacacat ttcctctcct
     2101 ggcctgccgg tggaacaagt gtttaaactg gtcctgcaag gtgttgagcg cgacaccaat
     2161 ggcaaacaat caccatggac atcttcttcc ttttccggga atttttcatt ccagcccaca
     2221 gtacaataag aacacacgca gatccattta gctctcccag ccccttatct tgtgtaagct
     2281 tcgggttgtg taaaaaagag aatccattca gaaatcttca gggaattttg aatgttctct
     2341 ccaaggatct tttccttcat gtagctcaca ccaatttcaa ggagacaaga gaagcgttag
     2401 aggtcttcaa tggcagcgtg acccaattga ccattgccgt gaatggccgg caagcatttt
     2461 caagcggcct ggctttttta cgaaagctag gcccaggggg ttgggttcag caacgattcg
     2521 cctgcaaaac ctcaattctt cttgacattc ctcacctaat ccattgggat aggtcatccc
     2581 tgagacattt tttatttcac aaaagacaaa ttctggtgaa ccgaattcca ttggggttcg
     2641 actcatcagg taaagactga ttattcaatt ttttaattca ccctctagtt caaaagggaa
     2701 aaaaggtatg aacatgtcct ttgccaaacg agttctctgt gcgatgttcg taagtattgt
     2761 ttttcaatcc ggtctccccg ctcaagccgt gatccccgat gaaaccacga ctatcacgct
     2821 ggaccaagcc gtccattttt taggcacgga tggcagtgat gtcgtggcag agccagggaa
     2881 ctattccgtc gaagcggcgc aggaatggct tcggctcatt cccggaaccg aacgccggga
     2941 tgccttgttg attgaagcgc aacaaggcac ccacgaggtg aaagtagaaa ttcctattgt
     3001 gatttccacc cctggcagtg aaccggacga attagatgtc cacgtggtgc aattcctcaa
     3061 tcctgacgga acgagtatgg tcgcaactgg gacttatagc ggcattcaat cacgagggct
     3121 atttgactcg gcaaagaaag cggcagccag agccaaggcg gtagcagaag cagctcgacg
     3181 agtcgccgcc cagaaggccg ccgccgctag ggccgcagcc ttacgagcca aacaagccgc
     3241 tgaacaagcc gcccaggctg cggctgctaa ggcccaagag ctggccaggc agacggctca
     3301 atttgcaaaa gttcaggcct gtaaaatcca ggtcagtgcc attagaggcg gtctcaaagc
     3361 tgggcaggca gcagaaagcc tgatgaagca agtggccgct attctcccag ttgccaggca
     3421 acggcaaggt tctctgcaaa atagactcaa tcaagaccag gcctatcgtg aacaaatcgt
     3481 gaacaccatg gcgtctggaa tcaatgccaa caaatacgtc attccggaaa tgaaccgcat
     3541 acaagccaat ttgaataatc cagccaaccg atcaaaattt atggatttac tatttagtcc
     3601 caccatgatt tgcgagaatt ctattgcgga cttggatagg gcattgattc aaacgggcct
     3661 ggcgcctagt tttgccatcg tgagaacccg tggcgcaacc gaaaatcatt tttacatggg
     3721 cttccaggtg acattggatg ccgcttatgt gggtggcgcc cagatcggat tcttcggagt
     3781 gactgatttt aggggaaatg ggggatggca ctggttcatt ggaccggaag cgggaatcgt
     3841 cgtaggggga gggggttccc tggaagttat tttctttcca aaggttaacc tggattcttt
     3901 tgcaggctgg ggagctggca ttggagggtc gcttggagca ggtgccactg gcaatgcaga
     3961 tgggatgtgg gacggtagtg tgaaagaatt tcagggtttt ggtgtcgggg gaggtggtgg
     4021 tgtcggatca aaaatggtcg gcagtctggc cgcttcctat acccacgcct ggaaaatcgg
     4081 aaaataacct aaaacagacg ttatcagtca tttcgttgac tatcccggcc tggcctccgg
     4141 aaggaaaccg ggccaatctt ctcaatcacc ggtttggata agcgaaaggg gtgtcaatta
     4201 tgaaggatag aaagttttct attttggtca cggcattaaa tgttgtcatc cttttcggct
     4261 tgtttacctc cggtcttgcc gaagatgcca atcaaagaat tcaagatcgc gtttcgcccc
     4321 cctcaacgag aaaaatcctt tcgccgtcag ggcgagaacc tgttacaaaa gcattaccga
     4381 ccactgatca aaaattcaca acaatttttt cgaaactggc gacattggag caaattgtgg
     4441 cgtctctcca aaatcaggtc accgctcagg cacaactcat tacccaactc aaacaagtga
     4501 tctccgtcaa ttcatcaggc accgtcacca ttcaggcacc gggcaccctc aaaattgccg
     4561 caggaggaaa cctgaacctc tcgggtagca ccgtggatgt gaacaccgca attaccggca
     4621 tccatggaat tcttaaagcc gacaccatga tgaccaccac ggtcatcgcc aaaagctata
     4681 cccctggagc cggcaacatt tggtaacctt cacattttat tgtcgtgaac attcacctcc
     4741 tatgggacga gccccatggt gatcagattc cacacttggc gagttgtccc tgaggacttc
     4801 ccgcaataag aatgcggtga tcctcaaaaa ttctgatgca ggcggaaaat gtgggtacca
     4861 gggaaaaaag caactcgacg aatttcgagt tatgaggtgt gagaataccg atacagtgtt
     4921 ttgatggact gaatcccatc tcgccagcgg atttttttcc cgtccgcttt acttctgggc
     4981 aaataagaaa tcggcacctc tgtcacgcgc caattctttt gagccacttt cagtgtgagc
     5041 tccaactcca attcaaatcc tgattcttcg agcgagatat cgctcaaaac ttcctgttta
     5101 aagactttgt aaccggtcca tacatccgtc aatgataacc ccgtcgcagc attggttaac
     5161 gttgtgaaaa tcttgttacc cagataggag accggtggcc aacccccttt ccagcccccc
     5221 aaaaacctgg acccgtagac aacatcggcc cggcctgtta tcaccggctc caacagtcga
     5281 tgatagtcct ctggactata ttccaaatcg gcgtcctgca cgatgacaat gctccctcga
     5341 acct
//
LOCUS       DATIAL010000168         3236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1321116, whole genome shotgun sequence.
ACCESSION   DATIAL010000168 DATIAL010000000
VERSION     DATIAL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321116"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VLA60_05195"
     CDS             complement(<1..231)
                     /locus_tag="VLA60_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08786.1"
                     /translation="MKSKSCQGHVWKIKNSRRVIREACGEVASPVGRLGIERIPPDLQ
                     AMRSQQRVHQLTVFSDPPFNPGSLKHYVSKNST"
     gene            complement(342..1430)
                     /gene="murG"
                     /locus_tag="VLA60_05200"
     CDS             complement(342..1430)
                     /gene="murG"
                     /locus_tag="VLA60_05200"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HSF08787.1"
                     /translation="MRVIIAAGGTGGHMFPAIAYAKEFQQHDPEGSIVFVGTGKSLEG
                     AILGKEGFGFEPIMVEGFVGRGLVGMARSLMLLPKSLWRAIQILRGHRADLVIGTGGY
                     FSPPVVVAAWLLNIPRVLLEPNAMPGLANRVLGPLADRIFLAFDSAKPFFSSSKVKVI
                     GTPIRKAFALERPPVPPQKISHLLIFGGSQGARAINSAMLKAVEISSKLREQVTITHQ
                     TGADDLERVKAGYAQLGVHVDVRPFLFDMPHELAKADLIVCRAGASTLAELSAYGKVG
                     ILIPFPQATHNHQEMNARSMEAAGAARVLLQSDLTGHRLAEEIERWMVDIPHLQEMAR
                     QSWILRKVNATEQMVSECLSLVGHPVAS"
     gene            complement(1448..2626)
                     /gene="ftsW"
                     /locus_tag="VLA60_05205"
     CDS             complement(1448..2626)
                     /gene="ftsW"
                     /locus_tag="VLA60_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HSF08788.1"
                     /translation="MGIPAILRRKNSHVESRAWADLFPGNLGIGSRRIDPLIVGITLA
                     LSLGGLVMVFSASGVMAENKFTNATYYLQRQIVWMLLGFGVLLVGSFIDYRRWKQWIP
                     LVVGGCIVGLLLVLAVGPQINGARRWLALGFFSIQPTEMAKLAVVLYLAAFLSNPQRR
                     VTDWQRGFLPPVAMVGIMCGLIVVEPDLGSTVVISLVFVGMMYLAGARLRHLAYLGGP
                     MILGVGALIWMSPERWERMTTFMNPFEDRQGAGYQLVQSILALENGGLFGVGLGQGKQ
                     KLMFLPEGHTDFVLALVGEELGLIGTCGLLALFAILVCKGFRVAALAPDLFGRYLALG
                     ITMLLGIQALINAGVVSGLLPTKGLTLPLVSYGGSSLLVTMLALGILLSVARQGRAGP
                     "
     gene            complement(2627..>3236)
                     /locus_tag="VLA60_05210"
     CDS             complement(2627..>3236)
                     /locus_tag="VLA60_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HSF08789.1"
                     /translation="GVYLEAGEVRARMHGKDFFIASRDALPMRGDHNVANAMAAMCVG
                     LLCGCPAEDMVRALQATPTFEHALEVVREWKGITFVNDSKGTNVDATLKALQSFHEPL
                     VLILGGKDKGGDFSQLADSIKRQVKGVVVIGEATQKILQALDQIKPVTLATSLTDAVS
                     QAVAFASSGDVVLFSPACASFDMFRNYHHRGLEFKRVVGELQ"
BASE COUNT          822 a          990 c          747 g          677 t
ORIGIN      
        1 tgttgaattt ttcgaaacat aatgttttag acttccaggg ttgaagggag gatcactgaa
       61 gacggtcagt tgatgcaccc gctgctggga acgcatggcc tgtaggtctg gaggtatcct
      121 ctcaataccg agtcggccta ccgggctcgc gacttcacca caagcctctc gtatgacacg
      181 acgactattt ttgatcttcc agacgtggcc ctgacaagac ttgctcttca ttccctgtaa
      241 gagtccctcc tcattaaaga ttgaatagct tcagacaaag gtttccactt tgactcgttt
      301 gtttattcct ccgtgccata tttccccatc atgcctgtat ctcacgacgc gaccggatgc
      361 cccaccagcg agaggcattc actcaccatc tgctctgtgg cgttgacttt cctgaggatc
      421 cagctctgtc gtgccatttc ctgtaagtgg ggaatatcca ccatccaacg ttcaatttct
      481 tctgccagcc gatgcccggt gaggtcggat tgcaagagca ctcgagccgc tccggccgct
      541 tccatggacc tggcattcat ttcctgatga ttatgtgtcg cctggggaaa cgggatcagg
      601 attccgactt ttccatatgc tgataactca gccaatgtgc tggccccggc ccgacagacg
      661 atgagatccg ccttggcaag ctcatgaggc atatcaaaga gaaaggggcg gacatctacg
      721 tgaacgccca attgtgcata gccggccttg acccgttcca aatcatccgc tccggtttga
      781 tgggtgatgg tcacctgctc ccgcagcttc gaggaaatct ccacagcctt caacatggcc
      841 gaattaatcg cccgagcccc ctggctcccg ccaaagatca acagatgtga gatcttctgc
      901 ggaggaacgg gaggacgttc caaggcaaat gcctttcgaa tgggtgtgcc aatcacctta
      961 actttcgatg aggaaaagaa cggtttcgca ctgtcaaagg ctagaaagat tctgtcggcc
     1021 aatggaccca acacccgatt ggccaatccc ggcatggcat tcggttctaa caagactctg
     1081 ggaatattca acagccaggc cgccacgacc acgggaggac taaaataccc gcctgtccca
     1141 atgaccagat cggcacggtg accgcggaga atctggatcg cacgccacaa cgacttcgga
     1201 agcagcatga gggaacgcgc catgccaacc agccctcggc caacaaatcc ttcaaccatg
     1261 atcggttcaa acccaaaccc ttctttcccc agaattgctc cctctagcga cttccccgtc
     1321 ccgacaaaca caatgcttcc ttcagggtca tgctgttgaa attctttggc atacgcgata
     1381 gccggaaaca tatgacctcc cgtcccaccc gcagcaatga tgacccgcat tacaccattc
     1441 tcttcactta agggccggct cgtccctgtc gagccacact cagcaagatt ccgagagcta
     1501 acatggtgac caggagggac gaccctccgt aactgaccaa gggaagcgtc aagcctttgg
     1561 tcggcaacag cccactcacg acgcctgcat tgatcagggc ctgaataccc aggagcatgg
     1621 taattcccaa cgcgagatat cgcccaaaca aatcaggtgc caaggccgca actcgaaatc
     1681 ctttacacac caaaatggca aacagggcca acaacccgca ggttccaatg agccccagct
     1741 cttctcccac caaggccaac acaaaatcgg tatggccttc cggaagaaac atcagctttt
     1801 gcttgccctg cccaagaccg acaccaaata atccgccatt ttccaaggcc aggatggact
     1861 ggacaagttg ataacctgca ccttggcggt cctcgaacgg attcataaat gtcgtcattc
     1921 gttcccatcg ttcgggactc atccagatga gagcacccac acccaaaatc atcggcccac
     1981 ctaaataggc caagtgccgc aatctcgctc cggccagata catcatgccg acgaacacca
     2041 ggctgataac gacggtactt cctaaatccg gctccacgac aatgagcccg cacatgatgc
     2101 ccaccatcgc cacaggggga agaaaccctc gctgccaatc agtgactcgc cgttgcggat
     2161 tggagagaaa cgccgcgagg tacagcacaa cagccagctt ggccatttcc gtgggttgta
     2221 tggaaaaaaa tccaagtgca agccatcgcc gagcaccatt gatttgaggg cccacagcca
     2281 ggaccagcag taatccaacg atacatcctc cgacaaccag gggaatccat tgcttccacc
     2341 ggcgatagtc gatgaaggat ccgaccaaca gaacgccaaa tcccaacagc atccacacga
     2401 tttgtcgttg gaggtaatag gtggcattgg taaatttatt ttccgccatg accccactgg
     2461 cactaaacac catgaccaat cccccaaggg acagtgccag agtgattccc acgatcagag
     2521 gatcgatccg cctggaaccg atgcccagat tcccagggaa cagatccgcc catgcgcgcg
     2581 attccacatg ggagttttta cgtcgcaaga tagcaggaat acccatttat tgaagttctc
     2641 cgacaacccg cttaaattca agtccgcgat gatgataatt ccgaaacata tcgaaactgg
     2701 cgcacgcagg ggaaaacaac accacatccc cactggacgc aaatgccacg gcctgggaca
     2761 ccgcatccgt caagctggtt gcgagggtca ccggcttaat ctgatccagg gcctggagaa
     2821 ttttctgcgt cgcttctccg atcaccacga cgcccttgac ctgtcgttta atactgtcag
     2881 ccaactgcga aaaatctccg cctttgtctt ttccaccaag aatcaagacc aagggctcat
     2941 gaaagctctg taaggctttg agggtggcgt ccacattcgt gcctttggaa tcattgacaa
     3001 aggtgatgcc cttccactcc cgaaccacct ctaaggcatg ctcaaaggtc ggcgtggcct
     3061 gaagagcacg aaccatatcc tctgcgggac atccacagag cagtccgaca cacattgccg
     3121 ccatcgcatt cgccacgtta tggtctcccc gcatcgggag agcgtctcgt gaagcgatga
     3181 aaaaatcctt cccgtgcatc cgtgctctca cctcgcctgc ttcgagatac actccc
//
LOCUS       DATIAL010000169         9959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_783520, whole genome shotgun sequence.
ACCESSION   DATIAL010000169 DATIAL010000000
VERSION     DATIAL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783520"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..3067
                     /locus_tag="VLA60_05215"
     CDS             377..3067
                     /locus_tag="VLA60_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HSF08790.1"
                     /translation="MTNEPNPSAETLSSSPSDTVWHTLSIPDVSQRLEVDPHTGLNAG
                     EARRRLQQHGQNTIPEHRQRSLARIFVDQFTDFMILVLIGAAIVSAMLGERVDALAIV
                     IIVGLNGIIGFVQEYRADRAMQAIKKLATPTTRVRREQQVASLSTLDVVPGDIVLLEA
                     GDLIPADLRLVEAVQLKADESALTGESVPVDKQTDPLEDLKLPLGDRRNMVYKGTLLT
                     YGRGTGVVIGTGLKTELGQIASLLHKDEDIKTPLQQRLAAFGRRLALAVLAICVVLFA
                     VGVWRGEPPVLMFLTAVSLAVAAIPEALPAVVTISLALGARKMARKQALIRRLPAVET
                     LGSVTYICSDKTGTLTQNSMRVEQMSVAGTILRPSAGRQPDSDADLRNPIHLFFLGLA
                     LNNDAKAQTEGQTLGDPTEVALYLAAEEAGYDKDTLESTHSRVQEIPFDSDRQCMTTM
                     HRMPKGTISFTKGSPEKLLPLCQSMLTQTGPVPLPKESLLEQAENMANEGLRVLAVAY
                     RRWDQAPSDMSPPVVERELTFLGFAGMMDPPREEAAQAVALCQSAGITPVMITGDHPG
                     TAKAIASRVGIINKDTMVLTGQELEHRSPDELAQLVEHTRVYARITPTQKIAIVKALQ
                     NRGEFVAMTGDGVNDAPALNQANIGIAMGQAGTDVAREASDMILLDDNFATIVTAVRD
                     GRRIYDNIRKFVKYTMTSNSGEIWTIFLAPLFGLPIPLLPIQILWINLVTDGLPGLAL
                     AMEPEERNIMQRPPRPPNESIFAGGLWQHILWVGLLMGGVSLATEMWAYQANQEQWQT
                     MVFTVLTLSQMGHVLAIRAEQEPFLQRGPLSNLWLFGAVLLTFALQMATIYVPILNPI
                     FHTMPLTLDQLGLCLILSTIVFFAVELEKLVRQRKRVSRQ"
     gene            complement(3322..3771)
                     /locus_tag="VLA60_05220"
     CDS             complement(3322..3771)
                     /locus_tag="VLA60_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08791.1"
                     /translation="MKGKPFRIFVFATALTFFGGSLHLPVALASSVLSFKVDVEIMVK
                     VSQKGFSDERGKLFGPKKFLKIPKGQVVRITFVFDESMSSLSYGDTHQIAITGAGGTK
                     ESEKIWMFSKKSSITLQSGKDGDAFRAYCIVDCIGMEHLNNLLIKVV"
     gene            complement(3908..4177)
                     /locus_tag="VLA60_05225"
     CDS             complement(3908..4177)
                     /locus_tag="VLA60_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08792.1"
                     /translation="MNENAPHATTNLECPNSRKKCDVNFEVNVFRGVTHGGVEAITCT
                     EFLHNNGVPTCGQECIHSSQAKNIHEEEVRKHQKELGKIGPNVIG"
     gene            complement(4908..7163)
                     /locus_tag="VLA60_05230"
     CDS             complement(4908..7163)
                     /locus_tag="VLA60_05230"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HSF08793.1"
                     /translation="MQFKRWMVDRLPPQFREWLWARKVEFGNRRIRELGCHLSNRIHQ
                     AAQQDLIQFERVVSNGEWELYRDKCLEALRRSLPDHSENLGTVSSMVTGTMEYAGCRI
                     DNIVFQGHRDLPVTANLYRPIRTTQKIPAIVICHSHHDSKTEEELQCMGMTWAQQGCV
                     VFVMDLLGHGERRQHPFGDPTGYGSSFRVDRQDYYFRSVLGMQLDMVGESLMGWMVQD
                     VRRGIDLLWADPQIDPDRIIVIGSVAGGGDLAAFVGALDDRVAALVAFNFGHLPMGDW
                     DSTRNLPDTARSGFWPWIILASLAPRRLVSGHEFAWNPEHDPVWARLEKVYELYGQRN
                     FLRSVHGSGRVSGHGPMDTHCTNVGPIHRGQLYSIFQEWFGIPIPDQEVNEPLPKEKL
                     ACLTRDTCRLFSLYSVHEVTQKICQKQLNSVRRVRASQESHARAVQLQHELVEVLGPM
                     TPFTPYRVRSIRKGFGRSEYVVLEVEKNLCVRLQLLWPSGLYATKSPVVIGIAQEGNF
                     PLRRERRSLIRSLLGQGIAVCLTELRGIGDGRHGEMYRGRISPSAGAAAISLMLGESL
                     GSSRVRDLRTVIDYLNNREDVDRSQIGLWGDSLAATNVVGKELAVPLDATPFPERGEP
                     LGGVATLLVALFEPQIQGVYVHGGLISFDVLLNEPFLYHPADSIIRGLLRIADLPDIA
                     AELVPRPLRMESLVDGYNRQASRKQIEETYHLVGLSYARAENPDRFSLQVEESSADTI
                     SRWFRHMFNLP"
     gene            7515..7853
                     /locus_tag="VLA60_05235"
     CDS             7515..7853
                     /locus_tag="VLA60_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HSF08794.1"
                     /translation="MVFTVLTLSQMGHVLAIRSEQNSFLQQGPLSNPLLHGAVLLTFA
                     PQMVTVYVPFLNPIVHTMPLTLEQLALCLFLSTVVFFAVELEKWVRRHGWLAQQPANP
                     NFQFSNEVPA"
     gene            complement(7960..8523)
                     /locus_tag="VLA60_05240"
     CDS             complement(7960..8523)
                     /locus_tag="VLA60_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF08795.1"
                     /translation="MFRFNLVAAVLVITLGMGWQKGLATAAEAHLITGYGFGHAPSDR
                     ELQGWDIDVRPTGAGLPAHQGTVSQGATVFAGKCASCHGATGREGPMDRLVDGQGTLA
                     SRQPIKTIGSYWPYATTLYDYIRRAMPFNAPQSLTNDEVYAVVAWLLYQNDIISTGTV
                     LDAETLPAVHMPNRNGFVPDPRPDVSR"
     gene            complement(8510..9787)
                     /gene="soxC"
                     /locus_tag="VLA60_05245"
     CDS             complement(8510..9787)
                     /gene="soxC"
                     /locus_tag="VLA60_05245"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HSF08796.1"
                     /translation="MHPAPEDPISETREEPLADIPGSRPLSRRALLCQAVTAVGLTAA
                     QAILSPLRAEKSSVPTDPTRVPGSLPTLYGQRSPFEKSERLVESWWGSFTPLQDLDGV
                     VTPASLHFERHHNGVPLIDPSHHKLLVHGLVDRPLILSMDDLKRFPSVSRLVFIECSG
                     NSGPEWRAPTGRTAQQTHGLTSTSEWTGVRLATVLKEVGVKPEATWMLAEGRDAAVMT
                     RSLPVADIVEEGLLCYAQNGEALRPEQGYPLRLVIPGWEGNTCIKWLRRLKLGTAPFM
                     TREETSRYTDLMPDGSARQFTMVMEAKSVITTPSGGQRVRPGFIEVRGLAWSGRGSVM
                     KAEVSTDGGRSWEEAQLQEPVLPKCHTRFRFSWRWDGKETILQSRCIDETGYVQPART
                     ALIAVRGMNSDYHNNAIQSWKISREGHVTNVQV"
BASE COUNT         2405 a         2865 c         2418 g         2271 t
ORIGIN      
        1 aattttggag gggacggacg ccagtctcag gagggcgggc caactcgctc cgctcaaaaa
       61 aggcccgcca gcttatggag cgtccctccc ttgggccagc cggcaggcgt cggaccatgg
      121 aagacgatac tttcaggaac tcacatgaaa gagcgaggga ccatatattc tgtatgatcc
      181 tgtaaaagtc tcacgggctg aaaaactatg tcatcctgag tgaaacgaag aatctggcca
      241 agccatggca ttttcaacct ggttagggcc tgactgagtc attacgggtt cgtctcatgt
      301 ctgaataatg aatctacgtt ttttgccact cacaatcagc aacacttcaa acacccctaa
      361 agacacacca ccaaagatga ccaacgaacc caacccgagc gcggagaccc tttcttcatc
      421 gccaagcgac acggtctggc atactctttc tatcccggat gtgagtcaac ggctggaagt
      481 cgatcctcac acgggtctga atgccgggga ggcccgccgc cggttgcaac agcatggcca
      541 aaacaccatt cccgaacatc gccaacgcag tcttgcgcga atcttcgttg atcaattcac
      601 cgattttatg atcctggtgc tcattggcgc cgccattgtc tcggccatgt tgggggagcg
      661 tgtggatgcc ctggcgattg ttatcattgt gggtctcaac ggcatcatcg gatttgtgca
      721 ggaatatcgg gcggaccgcg ccatgcaagc catcaaaaag ctcgccacgc cgacaacgcg
      781 cgttcgacgt gagcagcagg tcgcctcgct ctccacgctc gatgtggtac cgggcgacat
      841 cgtcctgctg gaagccggag acctcatccc tgccgatctt cgattagtcg aagccgttca
      901 actcaaagcg gatgaatccg cgctcacagg cgagagcgtt ccggtagaca aacagacaga
      961 cccgcttgag gacttgaagc ttcccctggg tgatcggcgc aatatggtct ataaaggcac
     1021 gctcctgaca tatgggcgcg gcacaggggt cgtcattggc acgggcctaa agaccgagtt
     1081 aggacaaatc gcgtcgctcc tgcataagga cgaagatatc aaaacgccct tgcaacaacg
     1141 cctggcggcg tttggacggc gcctcgcgct cgccgttctg gcgatctgtg tcgtgttgtt
     1201 tgctgtgggc gtctggcggg gtgagccgcc ggtgctcatg ttcctgacgg cggtgagtct
     1261 ggccgtggcc gcgattcctg aagccttacc agccgtcgtc accatttccc tggccttggg
     1321 cgctcgaaaa atggcgcgaa agcaggcgtt gatccggcga ctgcccgctg tggaaacctt
     1381 aggctccgtc acctatattt gctccgataa aacaggcacg ctcacacaaa attccatgcg
     1441 tgtggagcag atgtcggtgg ctgggaccat actgcgccct tctgcgggta gacaacccga
     1501 cagcgacgcc gacctccgca atcccattca tctgtttttt ctggggttgg cgctcaacaa
     1561 cgatgcaaag gcacagaccg aaggccagac cttgggtgat cccacggaag tggccttgta
     1621 tctggcggcc gaggaggccg ggtatgacaa agacacactg gaatccacac attcacgtgt
     1681 acaagaaatc cccttcgact cagatcgcca atgcatgacg acaatgcatc gcatgccgaa
     1741 gggcaccatc tcttttacca aaggatcccc ggaaaaactt cttccgcttt gccaatccat
     1801 gctcacacag accggccctg tgccccttcc caaagaaagc ctcctggagc aggcggagaa
     1861 tatggcaaac gaaggcctgc gggtcctagc tgtggcgtat cgccgatggg accaggctcc
     1921 ctcagacatg agtccaccgg tcgtcgaacg cgaactcacc ttcctcggat ttgccggcat
     1981 gatggacccg ccacgggaag aagccgcgca ggcggtcgcc ctctgccaat cggcagggat
     2041 cacccccgtc atgatcaccg gcgatcaccc cggcaccgcc aaagccattg cctcacgcgt
     2101 cggcattatc aataaggaca ccatggtgct gaccggccag gagttggaac atcgttcccc
     2161 tgacgaactg gcccaacttg ttgagcatac ccgggtctat gcccgcatta cacccacgca
     2221 aaaaattgcc atcgtcaaag ccctgcaaaa cagaggagag tttgtcgcaa tgaccggtga
     2281 cggggtgaat gacgcccccg cgttaaacca ggccaacatc ggcattgcga tggggcaagc
     2341 cggcacggac gtggcccggg aagcctccga catgattctg ttggatgata actttgcgac
     2401 catcgtcact gctgtccggg acggacgccg gatttatgac aacatccgca aatttgtgaa
     2461 atatacgatg acgagtaatt ccggagaaat ctggacgatt ttcctggccc ccttattcgg
     2521 cctgccgatt cccctgctcc cgattcagat cttatggatc aatctcgtca ccgacggctt
     2581 acccggcctc gctctcgcga tggaacccga agagcgcaac atcatgcagc gccctccccg
     2641 cccgccgaat gaaagcatct ttgccggtgg cctctggcaa cacatcttgt gggtgggcct
     2701 gctcatgggc ggcgtgtcgc tggccaccga aatgtgggcc tatcaggcta atcaagaaca
     2761 atggcaaacc atggtgttca ccgtactgac cctgtcccag atgggacatg tgctagccat
     2821 tcgtgcagag caggagccgt ttctccagcg agggccgttg agcaatctct ggttatttgg
     2881 agccgtcctc ctcacgtttg ccctgcaaat ggccaccatc tacgtgccta tcctcaatcc
     2941 cattttccat accatgccct taaccctgga ccaattaggc ctctgcctca tcctttcaac
     3001 aatcgtgttt tttgccgtag aactcgaaaa attggttaga caaagaaaac gggtttcccg
     3061 gcaataacca ttcctgctat accactctcg tggacaaaaa tcggagtgcg cgctgcttgc
     3121 tcttccgaaa acacgctacc cccgccatcg tatcaacaga ctcaaagctt ctcagtaatt
     3181 tttggtcaaa catgatgcgg ggacgcgggc atatctcatg gccaagggtc acactgaatg
     3241 gaacccgtcg cactcacaat tcaacattcc cgaaacgata tacccaacgt ttcagcgttg
     3301 taaccgtaaa tcaaaagccg gttaaaccac tttaattaaa agattattta aatgttccat
     3361 cccaatgcaa tcgacaatgc aatacgctcg aaacgcatcg ccgtctttcc cgctctgcaa
     3421 ggtaatactt gactttttgc tgaacatcca aattttttcg gactctttcg tcccgccggc
     3481 ccccgtaatg gcaatttgat gagtatcccc ataagacaag ctggacatgc tttcatcgaa
     3541 cacaaaggtg attcgcacca cctgaccttt cggaattttc aggaattttt ttggaccaaa
     3601 taattttcct cgttcgtctg aaaacccctt ttggctgact ttgaccataa tttcaacatc
     3661 taccttgaat gaaagtacgg atgatgccaa ggcaaccggc aaatgcaggc tgccaccaaa
     3721 gaatgtgagt gccgtagcga acacgaaaat tctaaatggc tttcccttca tgtatgttcc
     3781 ccctttatac gagtggtaaa atttctcttt gactatgtac ttttgtttca ttaatgatct
     3841 tgcgctgaag taactccctg gttatcaatt actacacata cacgcaagga catgctggtt
     3901 taaatgtcta tccaatgaca ttgggaccta ttttccccag ctctttttga tgcttgcgaa
     3961 cctcttcttc atggatattt tttgcctgag atgaatggat acattcctgc ccgcacgttg
     4021 gaacaccgtt gttgtgcaaa aattccgtac aggtgatagc ctccacaccg ccatgggtca
     4081 ctcctcggaa gacattcact tcaaaattga catcgcattt ttttcgggag tttggacatt
     4141 ccaagtttgt cgtggcgtga ggcgcgtttt cattcattgg tccattcctt ttcaattaaa
     4201 aagtaaggtg aaagcccgtg agcacttcac tgacggtcca tccattgctg tttattgttt
     4261 tgtattcccc gaggattgac agacccaggg aaacgacccg tcacctatcg gatatctgca
     4321 ggagagggta actcatatcc ctcatcgacg tggaaggaac tttccggcca aagaagccct
     4381 acaccagata agccatcaaa ggttatgtcc aatagtttta tcggaataaa agaaaaaaac
     4441 ttaaaggggt gaaaagggac aaaatcgaag tctcttaacc acacccaacg actaatgaga
     4501 caagatcgaa tttctatcgt accaagacaa tcaccatgcc caccttgtcc taaatgggcc
     4561 atgtgttggc gattcgtgcg gagggggaat catgtattca acgatgaccg ttaaacaatc
     4621 tctggttgtt aggaagcttg ctgctcacct ttgcgcttca gatggcgacc gtcaacgtgc
     4681 ggatacttaa tcccatcttt cataccaggc ccttaacctt ggaccaattg agcctctgcc
     4741 tcatgctttc aacaatcgtg ttttttgccg ttgaattcga aaaatggatc aagcgtcatg
     4801 gttggttggc caggtcataa tttggcagtc ccatctctgt catcatgacg agaaccttgc
     4861 catcattgga ggaaagacgc ttaatgccaa gtgaagtttg atttgagtca tggcagatta
     4921 aacatatgac gaaaccacct agagattgta tccgcagacg attcctcaac ctggagcgaa
     4981 aagcgatccg gattttctgc tcgagcgtaa gaaagaccaa ccagatgata ggtctcttca
     5041 atctgcttac gacttgcctg gcgattatac ccatccacta acgattccat tctaaggggt
     5101 cgaggaacca actccgccgc aatatccggc aagtctgcta ttcgtaacaa gccccgaatg
     5161 atagaatctg ctggatggta cagaaacggt tcattcagca atacatcaaa acttattagt
     5221 cctccatgaa cgtatacccc ttgaatctgt ggttcgaaaa gtgcgacaag gagagttgcc
     5281 actccaccta gcggctcacc tcgctccgga aacggtgtcg catccaaggg tacagccagt
     5341 tcctttccaa ctacgttagt ggcggcaaga gaatcacccc ataggcctat ctgacttcga
     5401 tcgacatcct cccggttatt gagatagtca ataacggttc gcaggtcacg aacccgggaa
     5461 ctccccaaac tttcccccaa catcagactt attgcagctg caccggcact cggactgatt
     5521 ctgccgcggt acatttcgcc atggcgacca tcacctatcc cccgcaactc ggtcaaacag
     5581 acggcgatcc cctgacctaa cagactccgg atcagcgaac gacgttctct tctcagggga
     5641 aaattcccct cctgcgcaat gccaatcacc accggtgact tggttgcgta caaacccgaa
     5701 ggccatagaa gttgcaagcg tacacaaaga ttcttttcaa cttccaacac aacatattcg
     5761 gaccgcccaa agccttttcg aatggatcga actcgatatg gtgtgaaggg ggtcattggc
     5821 ccaagaacct cgacaagctc atgctgtaat tgcacagccc gtgcatgtga ctcttgtgat
     5881 gctctcaccc tgcgcacgct gttgagctgt ttttggcaaa tcttctgcgt cacctcatga
     5941 acggaataca ggctgaataa cctgcacgtg tccctagtca aacatgcaag tttttccttg
     6001 ggaagaggtt cgttcacttc ttgatctggt atgggaatgc caaaccattc ttgaaaaatg
     6061 gaatagagct gcccacggtg gatggggccg acattcgtac agtgggtatc cataggacca
     6121 tgacctgata ctcggccgga accgtgaacc gatcgaagga aattgcgttg accataaagc
     6181 tcatacactt tttcaagacg cgcccaaacg ggatcatgtt ccgggttcca ggcaaattcg
     6241 tgcccggaga ccaaccggcg aggggccagg gaggcaagaa tgatccatgg ccaaaaacca
     6301 gatcgagcgg tatcaggaag atttcttgtg gagtcccaat cacccatcgg caggtgtcca
     6361 aagttgaacg caaccaatgc cgctacacga tcatccaagg ctccaacaaa tgccgcaaga
     6421 tctccgccgc ctgccactga cccaatgacg atgattcgat cagggtcaat ctgtggatcg
     6481 gcccataaca ggtcaatgcc acgcctcacg tcttgaacca tccaacccat caaactttcg
     6541 cctaccatat ccaattgcat gcccagcaca gaccggaaat aatagtcttg ccgatcaaca
     6601 cgaaaggagc tgccatagcc tgttggatca ccaaagggat gttgtcggcg ctcgccatgc
     6661 ccgaggaggt ccatcacaaa gaccacgcat ccctgctgtg cccaggtcat ccccatacac
     6721 tgcaattcct cttcagtttt tgaatcgtga tggctgtgac agataacaat cgcaggaatt
     6781 ttctgggtgg ttcgaatagg ccgatagaga ttggccgtca ccggcagatc cctatgcccc
     6841 tgaaagacaa tattgtcaat ccggcacccg gcatactcca tagtgccagt gaccatagag
     6901 gaaaccgtgc ccaaattctc tgaatgatct ggtaacgaac gacggagtgc ctccaggcat
     6961 ttatcacgat ataactccca ctccccattc gagactacac gttcaaattg aatcaaatcc
     7021 tgttgcgcag cctgatgaat ccgatttgag agatggcaac caagctctcg gattcggcga
     7081 ttcccaaact caacctttcg cgcccagagc cattcacgaa actgtggagg gagccgatcg
     7141 accatccatc ttttgaactg catagggccc attatgctct tccaacaatg ggttgactca
     7201 ttattgacag aaccatctga ttttccgagc tcgggcgaag gtatactcgc ctaactgata
     7261 tccgaaagct tagatttacc attgagtctg gtcagttcag aagcatccac ttgtcctctt
     7321 cttcaactca gtgggcacac agagattcga tctgacccat ccaataaacc ccatgcaatg
     7381 tccccccttc aaatgaaagt atttttgcgg gcagcctctg gcaatacatc ttgtgggtga
     7441 gcctgctcat gggcggcgtg tcgctcatca ccgaaatgtg ggcctatcgg gcagatctct
     7501 gacaatggca aaccatggtg ttcaccgtcc tcaccttatc ccaaatggga cacgtgctcg
     7561 ccattcgctc agagcagaat tcgtttctcc agcaagggcc gttgagtaat cccttgttgc
     7621 atggagccgt gctcctcaca ttcgcccctc agatggtcac ggtctacgtg ccgttcctca
     7681 atcccatcgt tcatacgatg cccttaaccc tggaacaatt agccctttgt ctttttctct
     7741 caaccgtcgt atttttcgcg gtggaactcg aaaaatgggt ccgccgtcac ggctggttgg
     7801 cccaacaacc tgcaaaccca aattttcaat tttccaatga agtgcctgct taggaagagg
     7861 ggtatccgga cggaccgcaa ctgcactccg gtcccggcgc aaacgaggac gatggacaga
     7921 atgcacgggc caggtttctt cagttatcac gaacgaagat tacctggaca catccggtct
     7981 gggatccggc acgaagccgt tgcggttcgg catgtgtaca gcgggcagtg tctctgcatc
     8041 cagcaccgtc ccggtcgaga taatgtcgtt ttgatacagc aaccaggcca cgactgcata
     8101 cacttcgtca ttcgtgaggg attggggcgc gttgaacggc atcgcccgac gaatgtagtc
     8161 atagagcgtg gtagcatagg gccaataact gccgatcgtc ttgatcggtt gtcgactggc
     8221 aagcgtgccc tgtccatcga cgagacgatc catcggccct tcccggccgg tcgccccatg
     8281 acaggatgcg cacttcccgg caaacacggt tgcgccctgg ctcaccgttc cctggtgggc
     8341 gggaaggccg gcaccggtcg ggcggacatc aatatcccag ccttgcagtt cacggtcgga
     8401 aggcgcatgg ccgaatccat aacccgtgat caagtgggct tcagcggccg tagccaaccc
     8461 cttctgccat cccatgccaa gcgtgatcac aagaaccgcc gcaactaaat taaacctgaa
     8521 cattggtgac gtgcccctct cgcgaaatct tccagctctg aatcgcgttg ttatgatagt
     8581 cagagttcat tccccggact gcgatcaacg ctgtccgtgc gggttgcacg taaccggttt
     8641 cgtcgataca gcggctttga aggattgtct cttttccatc ccagcgccag gaaaaccgga
     8701 acctggtgtg acatttcgga agaaccggtt cctgtagctg cgcctcttcc cacgaacgtc
     8761 ctccgtccgt gctgacctct gccttcatca ccgatccgcg accactccaa gcaagacccc
     8821 tcacttcaat aaagccgggt cggacccttt ggccaccgga aggagtggtg atgacggact
     8881 tggcctccat gaccatcgtg aattgtcgtg cgctgccgtc gggcatcaaa tcggtatacc
     8941 gggatgtttc ctcccttgtc atgaacggtg ccgttcccag cttcagtcga cgcagccact
     9001 taatacatgt gttgccttcc caaccgggaa tgaccagtcg tagcggatac ccttgctccg
     9061 gcctaagcgc ctcaccgttt tgcgcataac agagaagacc ctcctccacg atgtcggcca
     9121 ccggcagact acgcgtcatg accgccgcgt cacggccttc cgccaacatc caggtcgcct
     9181 ccggttttac gccgacttct ttgaggactg tcgccagccg cacccccgtc cattcacttg
     9241 tactcgtaag cccatgggtc tgttgggccg tccgtccggt cggagcccgc cattcaggtc
     9301 cggaatttcc cgaacattcg ataaagacga gtcgcgagac ggaaggaaac cgtttcaggt
     9361 catccatgga tagaatcaac ggccgatcga ccagaccatg aacaaggagc ttatggtgag
     9421 acggatcgat caacggcacc ccattgtggt ggcgctcgaa gtgtagggag gcgggagtga
     9481 ccacgccatc aagatcctgc agaggggtaa aagaccccca ccaggactcc accaaacgct
     9541 cactcttttc aaacggcgag cgttgaccgt agagagtagg cagggaaccg ggtactctgg
     9601 tcgggtcggt cggcaccgat gacttttctg cccgcaaagg gctcaagatg gcttgagccg
     9661 ccgtcagtcc gacggcagtg accgcttggc acagcagagc gcgacgactg agtggccggc
     9721 tgccgggaat atcggcgagc ggctcctctc gtgtttctga aataggatcc tcgggtgccg
     9781 ggtgcataga tgccccccat aaaccggagt gttggaccac cttgagtttg aaaattctgc
     9841 gccatatcac aagggcagac ttcagaccat cctacaatct tcttctcgtg tggacaagcc
     9901 actgccacgc gtcacgcatc ggcattatca ataaggacac cacggtgctt accggccag
//
LOCUS       DATIAL010000170         2915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1213730, whole genome shotgun sequence.
ACCESSION   DATIAL010000170 DATIAL010000000
VERSION     DATIAL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213730"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VLA60_05250"
     CDS             complement(<1..608)
                     /locus_tag="VLA60_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08797.1"
                     /translation="MIPNSRGPTSSYFKFRVLSQWVLLLMVFLDGCTGLFSGSLRPVE
                     EEQGRRILEALRQKETTIRTLRGLFQASISGSGLPFSQNILGTVSYRQPDVVHLKGFV
                     RLGVPVMDFHREGNDYKLYFPAEGKVVTGRMDESDDLTEWDQTVMLSIHALDAVLGKI
                     SGLSSAEVQVWTTEDQYRIDMAASQSSSPSAGDDFAVRSWVDA"
     gene            complement(598..1974)
                     /gene="radA"
                     /locus_tag="VLA60_05255"
     CDS             complement(598..1974)
                     /gene="radA"
                     /locus_tag="VLA60_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSF08798.1"
                     /translation="MKAARPKTRFVCQECGYQGVRWSGRCPECAQWNSIREEVDRDLS
                     TLQRNIAGGIPEAVVLGSIEQTAEFRLQSGIEELNRVLGGGVVPGSVILIGGDPGIGK
                     TTLLLQTLASLGTPEQIGLYVSGEESPQQIKMRADRLRIQSPHVYVLAATSLEEIFKV
                     ADQVNPGVIVVDSIQTVSTQELTSAPGSVSQVQEVGCRLMWYAKRTNVPIFIIGHVTK
                     EGVIAGPRLLEHIVDTVLYFEGDKGQSYRILRAVKNRFGPTNEIGVFEMKDEGLTEVD
                     NPSEMFLTGRIGQSAGSVVVSSVEGSRPLLVELQALVAETSYPMPKRMAKGVEVNRVA
                     LLLAVMEKRLGLHFTGYDVYVNVVGGLRIDEPTVDVGIVCAVLSSFREIALNPRLAVM
                     GEVGLGGEVRPVQHAELRIREAMKLGFTRCVVPEPNLEQFKSVPGMEVVGIREIGDIW
                     ETVVSHDP"
     gene            complement(2079..>2915)
                     /locus_tag="VLA60_05260"
     CDS             complement(2079..>2915)
                     /locus_tag="VLA60_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08799.1"
                     /translation="TFTPGRMFGQLVWLIVWNVSLGHTAELSVTDGLTSPGKSIQLQA
                     RLTNPEAEGEIGIPDQILEFIVHGRLAGQAKTDAQGWARLDFAPSMRGNLPVIVKRVT
                     QSKTEAVEGKGVLLSWERRRPILLIDLAVLVAGELEAAAPPTELAQDPGLDLGDAHPE
                     AAAELDKLAEFYYNLVYLDLTGRGRLETIQTWLRHHQFPPGMVRILSPTPTALTDLLH
                     DLKLEGWEKLSGGIGRTAEFAEILVQNRLHTVILPLPNMQERLPRRAIVLNDWSRIRR
                     HL"
BASE COUNT          734 a          878 c          638 g          665 t
ORIGIN      
        1 gcatccaccc atgaccggac tgcgaagtcg tccccagccg aaggggagga tgattggctt
       61 gctgccatat caatgcgata ctggtcttct gttgtccaga cctgcacctc ggcactcgac
      121 aatccagaga tttttcccaa cacggcatcc agggcatgga tgctcaacat cacggtttga
      181 tcccattccg tgagatcatc ggattcatcc atacgtccgg tgaccacctt gccttcagcg
      241 gggaaataca atttgtaatc attaccctcc cgatgaaaat ccattacggg aactcccaaa
      301 cggacgaaac ctttcaaatg gaccacatcc ggttgtcgat acgacaccgt gccgagaata
      361 ttttgagaaa aaggcagtcc ggaaccggaa atggacgctt gaaataatcc tcgtagtgtc
      421 cgaatagtcg tctctttttg acgcaaggct tcaagaatcc gtcggccttg ctcttcttcg
      481 actggtcgta gggaacccga gaacaatccg gtgcacccgt ccaaaaacac cattaacaat
      541 agaacccatt gactcaacac tcgaaacttg aaatagctac tggtgggacc tcgcgagtta
      601 gggatcatga ctgacgaccg tttcccaaat atctcctatt tcacggatac ccaccacctc
      661 cattcctgga acagatttga attgttccag attgggctca ggcaccacac aacgggtaaa
      721 tcccaatttc atggcctcac gaatgcgaag ttcggcatgt tgtaccggcc ggacttcacc
      781 acctaaccct acctctccca tcaccgcaag tctgggattc aacgctattt ctcgaaaact
      841 ggaaagcacc gcgcacacga ttcccacatc aaccgtcggc tcatcgatcc gaagcccacc
      901 caccacattc acatacacat catacccggt aaagtgtaaa cccaggcgct tttccatgac
      961 tgccaacagt aaggccaccc gatttacttc cacgcctttt gccatgcgtt tcggcatggg
     1021 ataactcgtc tccgccacga gagcctgcaa ttctactaat aaggggcgcg atccttccac
     1081 gctggacacc accacggatc cggcactctg gccgattcgc ccggttaaaa acatctctga
     1141 aggattatcc acttctgtca gcccctcatc cttcatttca aacactccga tctcattcgt
     1201 aggcccaaac cggttcttca ccgcgcggag aattcgataa ctttgtcctt tatcaccttc
     1261 aaaatacagc acggtatcga cgatatgttc caggaggcgc ggcccggcga tcaccccttc
     1321 cttcgtcaca tgacctataa taaagatcgg cacattcgtt cgcttggcat accacatcaa
     1381 gcgacagcca acttcctgta cctggctcac actacccgga gctgacgtga gttcctgagt
     1441 agagaccgtt tgaatggaat cgacgactat gacaccagga ttgacctggt ccgccacttt
     1501 aaaaatttct tccagggaag tggcggccag cacatacaca tgcggcgatt gaattctcag
     1561 gcgatccgca cgcattttga tttgctgcgg agattcttct cccgatacat ataatccgat
     1621 ctgttcaggt gttcctaaag aggccagcgt ttgtaacaga agagtcgtct tgccaatgcc
     1681 aggatcaccg ccaatgagaa tcaccgatcc gggaaccacc cccccgccaa gcacacgatt
     1741 taattcctca attccggatt gaagacgaaa ctccgcggtt tgctcgatgg agccgagcac
     1801 taccgcctcc gggattccac cggcgatatt tcgttgaagt gtcgaaagat cccgatcaac
     1861 ctcttctcgt atggaattcc attgggcaca ttccggacac cggccactcc accggacccc
     1921 ttggtatccg cactcctgac acacaaaacg ggttttggga cgggcggctt tcatctagtg
     1981 actccggata accggatagt taactttcag aagaataaag aagaccgtag aaaatggcga
     2041 ccaccgcttc gcaattccca ccggccgcta ttccgacatt acagatgtcg tcgaatacgg
     2101 gaccaatcat tcagcacgat ggctctacgg ggtaagcgct cctgcatatt gggaagcggc
     2161 aaaatcaccg tatgcaaccg attttgaacg aggatttccg caaattcggc cgtacgtccg
     2221 atgccaccgc tcaacttttc ccaaccttcc agtttcagat cgtgcaagag atccgtcagt
     2281 gccgtgggcg tgggagaaag aattctgacc attcctggtg gaaattgatg atggcgcagc
     2341 caggtctgaa tagtttccaa gcgacccctc ccggtcaagt ccagatacac aaggttgtaa
     2401 taaaattcag ccagcttatc caattctgca gccgcttccg gatgagcgtc acctaaatcc
     2461 aatcctggat cctgagctaa ttccgtagga ggcgctgcag cttccagttc gcccgccacc
     2521 agcaccgcca gatcaatcaa taaaatcggc cggcggcgct cccaggagag taatactcct
     2581 tttccctcca cggcttcggt tttcgattga gtcacccttt tcacaataac cggtaaattc
     2641 cctcgcatgc tgggagcaaa atcgagccga gcccatcctt gtgcgtcggt ttttgcctgg
     2701 cccgccagcc tcccatgaac aataaactcc aaaatctgat cggggattcc gatctcacct
     2761 tccgcttctg gattggtcaa acgcgcctgt aattgaatgg acttcccggg agaggttaac
     2821 ccgtcggtca ccgacagctc agcagtatgg cccagagaga cattccacac tatcagccac
     2881 accaattgcc cgaacatccg ccccggagtg aatgt
//
LOCUS       DATIAL010000171         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_676215, whole genome shotgun sequence.
ACCESSION   DATIAL010000171 DATIAL010000000
VERSION     DATIAL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676215"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VLA60_05265"
     CDS             <1..564
                     /locus_tag="VLA60_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HSF08800.1"
                     /translation="DLIELGTQADFSVEPIAPVIVGQKVVSSTRIRQCLTVGDVRQGA
                     QCLGRPYMLEGIVIPGEKRGKQFGWPTANLRLPGHRVLPADGVYATLMVLKGECLDSI
                     AYIGTRPTFSEEERLLEVHVFDKTLQLYGEDISVHFIERVRGDMVFANSQDLVSQMAQ
                     DGQRAREILRNYSGGPRIPVVVQGESV"
     gene            565..1992
                     /gene="tilS"
                     /locus_tag="VLA60_05270"
     CDS             565..1992
                     /gene="tilS"
                     /locus_tag="VLA60_05270"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HSF08801.1"
                     /translation="MNRLGPGVGMERKVDQAIRAKGLLASGDRIVVAVSGGPDSVALF
                     RCLVELRIRWHWDICIGHVDHGFRGAESEGDAKFVEVLGERFGVPVMVRRLHLNKYDA
                     KLKKESLQEYARKARYRALEQMVLDRKATKLVLGHTADDQAETVLMWMLRGCGTGGLG
                     GIPPKRGMYVVRPLLDIHRSEIVEYLEERQEEFRVDSSNVQPVYLRNRIRQHLIPQLK
                     QYSPGIVNVLTRQAHILREDHAYLETLADEAFERTCVSDVMGERQFDRMALLNVPLPI
                     RRRVVRQSLQIIAGHQQSPRFDLVERVLDRLAHGQSGWTIVCNGVRVGQEYDRLVMCS
                     CEEMHQPRRDFSGMSVMSLSIPGEVVWLPSGHRFSLSRESFPVMNSQAHHLEIYLDPA
                     RFTPELMLRSWMPGDIFCPKGLGGRQKKIQDFFSDLKLPRSQRNKVPLLVAPEGIVWV
                     AGLRADERFQVSSSTTSVVMARMIM"
     gene            2006..2557
                     /gene="hpt"
                     /locus_tag="VLA60_05275"
     CDS             2006..2557
                     /gene="hpt"
                     /locus_tag="VLA60_05275"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSF08802.1"
                     /translation="MNQIFGKPLVTQEAMRARIKDLGKQISQDYQDKDLLVVGVLKGA
                     YVFFADLVRVIRLPIQIDFLIAKSHKSIGSSAKKVKVWSELTEKINNRHVLLVEDIVD
                     SGITAQFLVKTLMKKKPASVAVCALISKSANRKVEVDLRYVGFEVPSTYVVGYGLDFK
                     QKYRNLPYLAKLDPKLVREDSQS"
BASE COUNT          610 a          510 c          760 g          681 t
ORIGIN      
        1 gatctcattg aattggggac acaagcagat ttttcggttg agccgattgc gcccgtaatt
       61 gtgggacaga aggtggtgag ttcgacgcgc attcgccagt gcctgaccgt tggagacgtg
      121 aggcagggtg cacaatgttt ggggcgcccc tatatgttag aggggattgt gattcccggc
      181 gagaagcgag ggaaacagtt tgggtggccg acggccaatc ttcgactccc cgggcatcgt
      241 gttcttcctg ccgatggtgt gtatgccacc ctgatggtgt tgaaagggga atgtctggat
      301 tccattgcct atatcggcac gcggccgacg ttttctgaag aggaacgttt attggaagtg
      361 catgtgtttg ataagactct tcagctatac ggagaagata tttcagtgca ttttattgaa
      421 agagtgcgag gggatatggt gtttgccaat tcccaggatc tcgtgagtca aatggcccag
      481 gatggccagc gagccagaga gatcctgcgg aactattcag gaggaccccg gattcctgtg
      541 gtcgttcagg gggaaagcgt atagatgaat cgtctaggtc ccggtgttgg aatggaacga
      601 aaggttgacc aggctattcg ggctaagggg ttattggcct cgggagatcg gatcgtggtg
      661 gcggtttccg gcggtccgga ttctgtggcc ctttttaggt gtttggtgga gttgcgcatc
      721 cggtggcatt gggacatctg tattggtcat gtagaccatg gatttcgtgg agcggaaagt
      781 gaaggggatg cgaagttcgt ggaggtttta ggtgagagat ttggagtccc cgtcatggtt
      841 cgccggctcc atctgaataa atacgatgca aaattgaaga aggaatcact ccaggaatat
      901 gccagaaaag cgcggtaccg ggcattagag caaatggtcc ttgaccggaa ggcgacaaag
      961 ttagtgcttg ggcatacggc agacgatcaa gccgagacgg ttcttatgtg gatgctacgc
     1021 gggtgtggaa ccggtggatt aggcggcatc cctcccaagc gaggaatgta tgtcgttcgt
     1081 cccttgcttg atatccatcg aagtgagatt gtggagtatc tggaggagag gcaggaggaa
     1141 tttagagtgg attcctccaa tgttcaaccc gtgtacctcc ggaatcgcat tcgtcagcac
     1201 ctgattcctc aattgaaaca gtattcgcca gggattgtga atgtgttaac ccgtcaggca
     1261 catatacttc gggaggatca tgcatacctg gagaccttag cggatgaagc gtttgagcga
     1321 acatgtgtgt cagacgtcat gggagaacgg caatttgacc gtatggcgct tttaaacgta
     1381 ccgttaccta ttcgtcggag ggtggtccgc cagagtcttc agatcattgc tggacaccag
     1441 cagagtccga ggttcgactt ggtggaacgg gtattagatc ggttagcgca tggacagtcg
     1501 ggttggacga tcgtatgtaa cggcgtgcgg gttggacagg aatatgatcg tctggtgatg
     1561 tgctcttgtg aggaaatgca tcaacctaga agggactttt ccgggatgag cgtgatgtct
     1621 ttatccatcc ctggtgaggt ggtttggctc ccctcggggc accgtttttc gctttcaaga
     1681 gaatcatttc cagttatgaa tagccaggca caccacttag agatttatct tgatcccgcg
     1741 agatttactc ctgaattaat gttacgaagt tggatgcctg gagatatttt ttgccccaag
     1801 gggctgggtg gccgacaaaa aaaaatccag gacttttttt cggatcttaa attaccccgt
     1861 tcccagcgga acaaggtgcc attattggtt gcgcctgagg gaattgtctg ggttgctggg
     1921 ttacgagcgg atgaacgttt ccaggtttca tcatccacga cctcggtcgt tatggccagg
     1981 atgattatgt aatatggaag tctctatgaa tcaaatcttt ggaaaaccac tggtaacaca
     2041 agaagccatg agggcgcgca ttaaggacct gggaaagcaa atttctcagg attatcaaga
     2101 caaggatctt ttggttgtcg gggttctcaa aggggcctat gtgttttttg ccgacctggt
     2161 tcgagtcatc cgattgccta ttcaaattga ttttctgatt gccaaaagtc acaaaagcat
     2221 cggtagttct gccaaaaagg tgaaagtgtg gtcagagctg acagaaaaga tcaataatcg
     2281 ccatgtgttg ctggtagaag atatcgtgga ctctggaata actgctcagt ttttagtcaa
     2341 aacgttgatg aagaagaaac cagcctctgt ggctgtctgt gcgttgatta gtaagtctgc
     2401 caatcgcaag gtggaggttg atttgcgata tgtgggattt gaagtgcctt cgacctatgt
     2461 tgtgggatat gggctggatt ttaagcaaaa atatcgaaat cttccttacc tggctaaact
     2521 cgacccaaag ttggttcgag aagactcaca gtcttgatgc g
//
LOCUS       DATIAL010000172         5691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1106798, whole genome shotgun sequence.
ACCESSION   DATIAL010000172 DATIAL010000000
VERSION     DATIAL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106798"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1438
                     /locus_tag="VLA60_05280"
     CDS             233..1438
                     /locus_tag="VLA60_05280"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HSF08803.1"
                     /translation="MTVSAYIINDPNIRLWNLTSRTRYQRMLSRLGVGSFLNNLNELP
                     SDHSLLIIRGDYLFDGRIFSFLLKKTNVVLEVEAQAGLCPVVAHVDSSLASATCDGIQ
                     REHTRNVANLQSVTLQDLSTSFSNELRKTDHPYVFPIRENNRVALEEHLFTGSYKGVT
                     DLVTKFLWPVPAKWATRLCALWGISPNQVTSLSLFLVIAAGVLFAFGQFFWGLVLGWI
                     MTFLDTVDGKLARVTVTSSKWGNIFDHGIDLIHPPLWYLAWGVGLTAFAPLLSWLTVE
                     TTIGIILIGYVLGRMVEGIFQWWLGGFVIFCWKPLDSYFRLVTARRNPNLILLTASLL
                     FGRPDLGLEAVAIWTVGSTLILIARLLMAGWGKARMGSLTSWLAEIDPMNRNSLAVRM
                     FTNPPVTIK"
     gene            1531..2511
                     /locus_tag="VLA60_05285"
     CDS             1531..2511
                     /locus_tag="VLA60_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HSF08804.1"
                     /translation="MDWSTMHVGILHNALSGRNRRKPDLFQEIYNKYPEVQRAEVNTP
                     ADILESLKTFAHLQVDCVVVNGGDGTIQATMGALFHHRPFAVMPRLAVLPAGTANLIA
                     GDVGLGKFESNTLDQLLVQAEAMTPRLSFETRPVLRIRFPENREPLHGMFFGAGAIYH
                     GTQLGLETKQSIGRLGEWGAGLILLKFLLALATGSRKGLNPITVGVATGESAPLKDEY
                     LVLLVTTLNRLFLGMKPFWGKQAWPLHYTSLRVPYRYLWRALPTLFRGTTHPLATTEH
                     GYDSENLSELRLVFNSGFVLDGEVYASSMPEEPLTLDSPGELSFVRLKTD"
     gene            2517..3470
                     /locus_tag="VLA60_05290"
     CDS             2517..3470
                     /locus_tag="VLA60_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08805.1"
                     /translation="MNGSPTVLEIVSQPSARPIPASIQAAGNFLATKYGQAVQGILLY
                     GSCLRAGTDQDGLVDCYVIVDQYASVYQSAWLALLNRWLPPNVFYGEVNLEERIVRMK
                     YAVLSLEDFERSVTPKWFHSYFWGRFAQPTAVMACPNQAMSQRIILGLGRAVLTFLGN
                     VVPQMPQQFSAKDAWSIGLALSYGAELRAESQGRIATLWDSNQAYYEQVAHETFLAHF
                     PKIKVIQRDDITMYEIEYSRVDYTKNRIAWAVRKVQGKVLSILRLMKAAFTFQGGADY
                     LIWKIERHSGVKIELTPAQRRHPIWAGLTTFWRLYRQGAFR"
     gene            3877..4293
                     /locus_tag="VLA60_05295"
     CDS             3877..4293
                     /locus_tag="VLA60_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08806.1"
                     /translation="MIPEVQHIRIRCLEEGTQKIINLYTYTALLLNACDVAVKGYEAR
                     KDNFQLSTTMCIPDPLRLPVEVERKVEVVFHRNDLPSVYRDQAIQRLAEDFMIRMVAV
                     MDDVLEDIYEKTLPLIFLSLSDADIAKRVRSSWGQD"
     gene            complement(5038..>5691)
                     /locus_tag="VLA60_05300"
     CDS             complement(5038..>5691)
                     /locus_tag="VLA60_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSF08807.1"
                     /translation="PMYERLVRLGQGSNQTCACRLVCLRVTAQVAQARRRRFKAHARR
                     QGRTGSHRHVALCDWTLLITNVPEPWLPLDMVRALYTLRWQIELLFKQFKSILRVHQS
                     ATGNIHRLRCELYGKLIAAVWVQRLHAVANTTLWNTARREISLAKLYKRLQERAFLLS
                     QLVMGSEAQARGYLSHELETLLRHCRKHRQRSRMTTLEMLEAGFDPKLHRGKGQGLA"
BASE COUNT         1366 a         1254 c         1492 g         1579 t
ORIGIN      
        1 tatgggtgtc tggaaatcgt cgataatatg tacgggtatt cctttgatga ggcataagca
       61 atgttgtata tcaattcagg catatgtccc agggatttgt gggcatgaat gccgattgca
      121 gtcatagacg ggattatggt cacaatcata tttgttcccg tcagttatta tgggagagtc
      181 gggcgactct attattcttt aatgtgtaag gggaataagg cgtggtagtt caatgactgt
      241 gtccgcatat attatcaatg atccgaatat ccgtttgtgg aatttgacct cccgtacccg
      301 gtatcaacga atgttaagtc gattgggggt tgggagtttt ctcaataatc tcaacgaact
      361 tccatccgat cattcgcttc tgattattcg aggggattat ttatttgatg ggcgaatatt
      421 ctcctttctc ttgaagaaaa ccaatgttgt tctggaagtt gaggctcaag ccgggttatg
      481 tccggttgtg gctcatgtgg attcctcttt ggcttccgca acgtgtgatg ggatacaacg
      541 agaacacaca cgaaatgttg caaacctgca atctgtgaca ttgcaggatc tttcaacgtc
      601 cttttccaac gaattgcgaa aaactgatca tccctatgtg ttccccattc gtgaaaataa
      661 ccgggtggct ttggaagaac atttatttac ggggtcgtat aaaggcgtca ctgatctggt
      721 aacgaagttt ctttggcccg ttccggccaa atgggccacc cggctttgtg cgctttgggg
      781 gatttcgccc aatcaggtga catcgctgag cttattcctt gttattgcag caggcgtgtt
      841 gttcgctttt ggtcagtttt tctggggact tgtcctgggt tggatcatga cgtttctcga
      901 tacggtggac ggaaaattag ctcgagtaac ggtgacctcc agtaaatggg ggaatatttt
      961 tgatcatggc attgatttaa ttcaccctcc actttggtat ttagcttggg gagtggggct
     1021 caccgcattt gcccctttgc tttcctggtt gacggtggaa accaccattg gaatcatttt
     1081 gatcggatat gtattgggac ggatggttga aggaattttt caatggtggt tggggggatt
     1141 tgtcatcttt tgttggaagc cgttggattc ctatttcagg cttgtcacag ctcgacgtaa
     1201 tcctaatctc atcctcttaa cggccagtct tctttttgga cgtcccgatt tgggcttgga
     1261 ggctgtggcc atttggacgg tgggctcaac cctgattctt atcgcgagac ttcttatggc
     1321 gggttggggg aaagccagga tgggttccct cacttcctgg ttggctgaga tcgatccaat
     1381 gaatcgaaac tccctggcag tcagaatgtt taccaatcct ccggtcacga tcaaatgatc
     1441 tttcatgcgg actttacaca tcttttcgtc ttgcccgtat tccccaatgt caatgattcc
     1501 tatttcatct ttgccgtcgc ttgaggtcgg ttggactggt ctacgatgca tgtggggatc
     1561 cttcataatg cgttgagtgg tcgtaatagg cggaagccgg acctttttca agaaatttac
     1621 aacaaatatc cagaagtaca gcgggctgag gtcaacacgc ctgcggatat cctagaatct
     1681 ctgaagacgt ttgctcacct tcaggtggat tgtgtggtcg tcaatggagg ggatgggacc
     1741 attcaagcca ccatgggggc tttgtttcat catcggcctt ttgctgtcat gccccggttg
     1801 gctgtgttgc ccgcagggac ggccaatctg attgcggggg atgtgggttt agggaaattt
     1861 gaatcaaaca cacttgacca attgttggtt caggccgagg ccatgactcc gagattatct
     1921 tttgaaacgc gaccggttct tcgtattcga tttccagaaa accgcgaacc cctgcatgga
     1981 atgtttttcg gggctggagc gatttatcat gggacccaac taggtctcga aaccaaacag
     2041 tcgataggcc ggttagggga atggggcgca gggctgattc tgttgaaatt tttattggcc
     2101 ttggccaccg ggtcccgaaa aggtttgaat ccgatcacgg tgggtgtggc aactggggaa
     2161 tcagcacctc tcaaggatga gtatttagtc ttgctggtca ccaccttgaa tcgcttgttt
     2221 ttagggatga agccgttttg gggcaagcaa gcctggcctc ttcactatac gagtcttcga
     2281 gtgccgtacc gctatttgtg gcgtgcctta ccaaccttat ttcgtggaac gacgcatcct
     2341 ttagcaacga cagaacatgg ctatgacagt gaaaaccttt cagagcttcg cttggtgttt
     2401 aacagtggat ttgtcctgga tggcgaagtg tacgcctctt ccatgcctga agagcctcta
     2461 accctggatt ctcccggcga gctgtcattt gtccggttga agactgatta acggtgatga
     2521 acggttcccc aacagtcctc gaaatcgtta gccagccgag cgctcgaccc attcctgcgt
     2581 ccattcaagc agcgggaaat ttcctggcta caaaatacgg gcaggccgtt caaggaatct
     2641 tattgtatgg ctcgtgcttg cgggcgggaa cggatcagga cggattagtg gattgttatg
     2701 tcattgtgga tcagtatgcc tcggtctacc agtctgcttg gttagccctc ctcaatcgtt
     2761 ggctcccacc taatgtgttc tatggagaag tgaacctcga agagcgaatt gttcgtatga
     2821 agtacgccgt gctctcgtta gaagattttg agcgaagtgt gactccaaag tggtttcatt
     2881 catacttttg ggggcgcttc gcgcagccga cggccgtgat ggcctgtccg aaccaagcga
     2941 tgagtcaacg gattatttta gggctgggca gagcggtctt gacctttttg gggaatgtgg
     3001 ttccgcaaat gccccaacaa ttttctgcca aagatgcatg gagtattggt ttggccctca
     3061 gttatggcgc cgaattgcgt gccgaatcac aagggcgaat tgcaacattg tgggacagta
     3121 atcaggccta ttatgaacaa gtcgcccatg aaacgttttt agcacatttt ccaaaaatta
     3181 aggtcattca gcgggatgac ataaccatgt atgaaataga atattccagg gtggactata
     3241 ccaagaatcg catagcctgg gctgttcgca aagtgcaggg gaaggtgtta tctattttgc
     3301 gccttatgaa agccgccttt acgttccaag ggggggcgga ctacttgatt tggaaaattg
     3361 agcggcattc cggggtgaaa atcgaattga cccctgccca acgacgacat cccatttggg
     3421 ccgggctcac gacattctgg cgtctttatc gccaaggggc atttcgatag ataccgggtc
     3481 tcgcccgggc agccgagcca cttttgtttc ggcaaaagta gccaaaacca gtgacgccca
     3541 gtccggccag attgggaggg atgaacgcaa ggaagggagg cggaccaact cgctgcgctc
     3601 agacaaggcc cgccgaatga taggagcgtt catcccaaaa gccggacagc aggcgtcgtt
     3661 gttaattagt gcttgtggtc aaatattcct tagaaagttt aagtagttgc ggaagatttg
     3721 cgatacgaaa gaattggtta aaaagtatgg acatctgaga tgacaaaaat tactgaaaat
     3781 gtaaccgaat acggaattct gtaacattga gactggccat tattcggaaa aagtagaact
     3841 gccacctgct tgggttggca aatcgagaac ttttttatga ttccagaagt ccaacatata
     3901 aggatccgtt gtctggagga aggaactcag aaaataataa atttgtatac ctacactgct
     3961 ctgttactga atgcctgtga tgttgcagta aaagggtacg aagctcgtaa agataatttt
     4021 caattaagca caacaatgtg tatacctgat cctctacggc taccagttga ggtggagagg
     4081 aaagtagagg ttgtatttca tagaaatgac ttgccaagtg tttacagaga ccaggcaatt
     4141 caaagattag ctgaagactt catgattcga atggtggcgg taatggatga tgtcctagaa
     4201 gatatttatg aaaaaacact tcctttaatc tttttgtcct tgagcgacgc tgatatcgct
     4261 aaaagagtaa gatcatcctg ggggcaagat tagaacggac aagtaaatct ttttaatttc
     4321 ctagttaatg atgcaggcct taaatcacct acagtctggc catctacttt ggatatggtc
     4381 tttgatcgct attatgaaat gagagaaata agacacgcat tagttcatag tgcaggcata
     4441 ttatcagaga aaaacaaatt taggctgaac agtttagctg aacgactacc taaagattga
     4501 agggagattt ctttatcatc taaatcattt ttagctggtg gagttgtcaa attaaagtat
     4561 ccagatattt gatgctaaga cattgggcat ataccaccat aatgaattat ctccaaattt
     4621 cgtttaagga aagtgttgct tagctatgta taaactgttg gttttggtaa agagcaaaca
     4681 ttgaaaatgg aaccgaatac cccattcggt tacatgaatt ggtaattgga attcctcctg
     4741 tccatttttt aagcaacaag ccacatggaa ttggccattt actccgggct ttcggtataa
     4801 ggcctgtcaa ccactttatt cttggtgtgg tggggaatta tttacgacgc ttgctgtccg
     4861 accatgggga cgggcactct tctttcttgg cgggccttgt ctgagcaaag cgagttggac
     4921 cgcccttctt tatcccgcgg ccgtcctatt ctgatgaggt cggactgggc gtcaatggtt
     4981 ttgggtcatt ttgccgaaac aaaagggcct cgcctgccgg ggcgagaccc atagctgtta
     5041 agctaagcct tggccttttc ccctgtggag tttcggatcg aagcccgctt cgagcatttc
     5101 tagcgtggtc atccgtgagc gttggcgatg ttttcgacag tgcctgagca aggtctcaag
     5161 ctcatgggat agatagcccc tggcttgcgc ctccgacccc atcaccaatt gagagagcag
     5221 gaaggctcgt tcctggagcc gtttgtataa ctttgccagg ctgatctctc ggcgtgcggt
     5281 attccacaac gtggtgttgg cgacggcatg caggcgttgc acccacaccg cggcgatcaa
     5341 tttgccgtag agctcgcatc gcaaccgatg gatattgcca gtggcggact gatgcacacg
     5401 aagaatggat ttaaattgtt taaacagcaa ctcgatctgc cagcggagcg tatacaaggc
     5461 ccgcaccatg tccaagggca accagggctc cggcacattg gtaatgagga gcgtccagtc
     5521 gcacaaggct acatgccgat gactgcccgt ccggccttgc cggcgggcgt gggccttgaa
     5581 ccgtcggcgt cgcgcctggg ccacctgggc ggtcacgcgc aggcacacca accgacaggc
     5641 gcacgtttga tttgagccct gccccaggcg caccaggcgc tcatacatcg g
//
LOCUS       DATIAL010000173         8750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_999329, whole genome shotgun sequence.
ACCESSION   DATIAL010000173 DATIAL010000000
VERSION     DATIAL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8750
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999329"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="VLA60_05305"
     CDS             complement(<1..1993)
                     /locus_tag="VLA60_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08808.1"
                     /translation="MLTRPDPTPMRSSGPPSSSHSLIELRRQQIHQLFSLVPMAILAS
                     IINCGLVAFILMDVIPTQIVWGWIIGIVGINVLWSGLVLAYRRASKPLLHPSTWLAWF
                     VAGNGASGVIWGMAGIALYPPSSPSHQMFLALVLGGMAAGSTAVHAAYFPAFLAYSLP
                     TTLPLTYQFFAQGDPPHQAVGIMGFTFLAVITVTAYRNFFMIKNTLTLEKENFQLIHQ
                     LEQAHSQAEALNRSLSQEIQTRIDIEKELLHHKDHLESLVGIRTADLQKSEARYRMVV
                     ERISDVIWVMDLEGSRFSYISPSLERLLGYSGYALDTLSPETILTPASMVTLREAIQQ
                     ERVHHQSGTHEPNQSFLLVLEHQHHNGSSIWAEVRGSLLLDKHNLPIGITGVTRDVTE
                     RKKIEQEKQRLEAQLLRSQKLESVGNLAGGIAHDFNNFLTTILGNITLTKQILPFSKE
                     RQSYLERAERAALHAKHLTHQLLTFSKGGDPIKQPVALSELIKESSELALSGSSLICQ
                     TWADPNLWIVEADPGQINQVMQNLLMNAIQAMPQGGSITIRAENIQLSDPDVRSPLPL
                     SAGPYVKVTIADEGIGIAEDHLPKIFEPYFTTKSAGQGLGLASTYAIIKKHAGHVTVD
                     SQVGGGTTFTLYFPASPKATSSPKSLNLGIRRGQGKILVM"
     gene            2282..2992
                     /locus_tag="VLA60_05310"
     CDS             2282..2992
                     /locus_tag="VLA60_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HSF08809.1"
                     /translation="MKSFTLLQKTITDCTTCQRLTTYRQAIAKEKVKRFRDWEYWGRP
                     ISGFGDPKAQVFVVGLAPAAHGGNRTGRVFTGDRSGDWLYEALHAFGFANQPDSTHRE
                     DGLTLNDCYVAAVVRCAPPANKPTKAEFAACRPYLQEELRLLRRVKVVVALGKIAFDE
                     YLRTCQTMGCQVPSPRPKFSHGAQYALSWGVTLVGSYHPSQQNTFTGTLTRPMFHGIF
                     STVRKLLTPSRPLRAPLT"
     gene            complement(3001..3654)
                     /gene="fsa"
                     /locus_tag="VLA60_05315"
     CDS             complement(3001..3654)
                     /gene="fsa"
                     /locus_tag="VLA60_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HSF08810.1"
                     /translation="MKLYLDTGSVKEIREAASYGLVDGITTNPSLVSKEGRDFKDLLL
                     EICGIVDGPISAEVVSVEAAAMVKEGKELAKIHKNIVVKCPLIPEGLKATKQLSSEGI
                     RVNVTLCFSPTQALLAAKAGAWCVSPFIGRLDDISSNGMELIRQILTIYENYNFTTQV
                     LVASVRHPQHVVEAALTGGHICTMPFGVFQQLVKHPLTDNGLQKFLSDWEARPSNKA"
     gene            3989..4300
                     /locus_tag="VLA60_05320"
     CDS             3989..4300
                     /locus_tag="VLA60_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08811.1"
                     /translation="MIYKYVLIGLLGLVCAGCGGNPYLDASLDPAKFEGKDKAWFEEN
                     WGKPSGKSARFFGGETWVYSRISGGESRFPYFNFSPNQCHINLDFDKEGKLEDYDYTD
                     C"
     gene            4420..5181
                     /gene="pgl"
                     /locus_tag="VLA60_05325"
     CDS             4420..5181
                     /gene="pgl"
                     /locus_tag="VLA60_05325"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSF08812.1"
                     /translation="MSRTIIITPTIEELFVVAAKEVVQVVLDCRKAGRDCRVALAGGS
                     TPRGLYRLLTGAPYRTQISWDHLRVFWGDERTVPPDHQDSNFRMAKEALLSHVPIPSR
                     HVFRIEGEVPAHEAAARYETVLQEQFNLSSGEVPQFDLILLGMGPDGHTASLFPGTSA
                     VAESEKLVSAPWVEKLQTHRVTLTPPVLNAAKHVVFLVSGVDKATALQVVLEGPADPA
                     RYPAQVVNPPTGHMVWLVNQDAAGLLKRESMTSRV"
     gene            5197..5592
                     /locus_tag="VLA60_05330"
     CDS             5197..5592
                     /locus_tag="VLA60_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08813.1"
                     /translation="MKMVKAPEAFIHALHKKPMTCPIPDCSGSVAVEERSQSTDRVKS
                     FRLHCEQCDWHDTITGDEQVDPPWDEGSLMEITEEHLLHLEPVCPYDQAPVDFHSLPN
                     PRRRARYRITCFFCGRQEELDWPPEEAKR"
     gene            5693..6448
                     /locus_tag="VLA60_05335"
     CDS             5693..6448
                     /locus_tag="VLA60_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08814.1"
                     /translation="MKKIKKKKSASQVVPAFLVGWTHGNPPPPGYIAAWFDEAYGGPL
                     RIRFLTSNGHHHFEAAHTNWTAEVNIEPSTAIVEQWQGRLQWEQTQLAILFPPTNTAT
                     DRRDALLHVARMARGVTLLTDGTTYDVATGTYLNPSDWRDRDLEVFRITDHIQVEHRE
                     DVQRARLWFHTRGLTKFGLDEIETFQPLGLSSREVKDTIIRVADSLIEQGKNSKVGER
                     IILDGSGPQVMVVRHRTDPVYGTPLAFREFILE"
     gene            complement(6488..7030)
                     /locus_tag="VLA60_05340"
     CDS             complement(6488..7030)
                     /locus_tag="VLA60_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08815.1"
                     /translation="MKKSYLLSLVFLFVFPTLAHAGSYAPLADFSAVSKNPQCRLTTM
                     HESFLNSWTPPAQFFLVPPYPNAVLASAMPSGNAQIHGHAYQTIPSAVLLSPDPPEKI
                     LEFYKRSLGATWFQAEDIDTIYLYRLPRPVASGEALTRQLISKPGSIPNIAIDTHLSP
                     CDQAIGRGAQTRITVVSPPR"
     gene            complement(7261..8199)
                     /locus_tag="VLA60_05345"
     CDS             complement(7261..8199)
                     /locus_tag="VLA60_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HSF08816.1"
                     /translation="MSGELLVMLVLALLAFIILSKTAIVVPQQSAYVVERLGKYSSTL
                     NAGLHILIPFLDVIRYKHTLKEMAVDVPEQICITRDNVQVGVDGVLYIKVLDAERASY
                     GITDYRFAISQLAQTTLRSEIGKIDLDKTFEERTTINISVVNELDKASSPWGAKVLRY
                     EIRNINPPKDVLSAMEKQMRAEREKRAVVLASEGQRDAAINEAEGDKQQVIKASEAKR
                     QQQINEAEGEASAILAIATATSEGIRKVAEAIQLPGGFEAVQLRVAEQYIGEFGELAK
                     ASNTLVLPASVSDVGSMIALAMNVINQKPASAPKLS"
     gene            complement(8189..8638)
                     /locus_tag="VLA60_05350"
     CDS             complement(8189..8638)
                     /locus_tag="VLA60_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSF08817.1"
                     /translation="MGLVLIGLEILTLGGLGNFYFLFFGVAALMVSALTWLSLTEAAW
                     LQWLCFVLFGILSLLAMKKPLQGTGFIREKDEVPVDSMVGEFATVLENLDAQRIGKVE
                     LHGTSWTARNAGTSTLGKGSQAKVIRVEGLTLWIQADPMTQEESHVG"
BASE COUNT         2043 a         2100 c         2429 g         2178 t
ORIGIN      
        1 ccatgactaa aatctttcct tgtccccgac gaatgcccag gttcaaggat ttgggcgatg
       61 aggtggcctt gggggaggca ggaaagtaga gtgtgaaggt ggtgcctccg ccgacctggg
      121 agtccacggt cacatgaccg gcatgttttt ttataatggc ataggtggaa gccaagccca
      181 atccctgtcc cgccgacttt gtcgtaaagt atggctcaaa aatttttggc aaatgatctt
      241 cggcgatccc gatcccttcg tcggcgatgg tgactttcac atacgggccg gcagaaagag
      301 gtagaggact tctgacgtcc ggatcggaca attggatgtt ttcggcgcga atggtaattg
      361 atcctccctg gggcatcgcc tggatggcat tcatgaggag attttgcatc acctgattga
      421 tttgccccgg gtcggcttca acgatccata ggttgggatc agcccatgtt tggcaaataa
      481 gcgatgaccc ggaaagagcc agttcggaag attccttgat caactcggat aaggctaccg
      541 gttgtttaat gggatcgccg cccttcgaga aggtgagtaa ttggtgcgtg aggtgtttgg
      601 catggagagc cgcacgttca gctcgttcca agtaggactg acgttccttg gaaaaaggca
      661 ggatttgttt ggtcagagta atatttccca gaatagtcgt caggaaatta ttgaaatcat
      721 gggcgatgcc cccggcgaga ttccccaccg attccaattt ttgggaacga agcaattggg
      781 cttccaggcg ctgtttttcc tgttcaattt ttttgcgttc tgtcacatct cttgtgactc
      841 ccgtaatgcc tatggggaga ttgtgtttat cgagcaggag gcttccccgc acttccgccc
      901 agattgagga tccattgtga tgttgatgtt ccaagactaa caagaacgac tgatttggct
      961 catgagtgcc ggattggtgg tgaacgcgtt cttgttggat ggcctccctc agagtgacca
     1021 tggaagcagg ggtcagaatg gtctctgggg aaagcgtgtc aagggcataa cccgaatacc
     1081 ccaataaccg ttccaaagat gggctgatat aggaaaacct cgaaccctcc aggtccatga
     1141 cccaaatgac atcggatatt ctttcgacca ccattcgata ccgggcttcg gatttttgca
     1201 aatcggcagt tcgaatcccc acaagcgatt ccagatggtc tttgtgatga aggagttcct
     1261 tttcgatgtc gatacgggtt tggatttcct gacttaatga acgattgagc gcttcggcct
     1321 gagagtgggc ctgttccaat tggtggatta attgaaaatt ctctttttcc aaggtgagag
     1381 tattttttat catgaaaaaa tttcggtagg cggtgactgt tatcacggcg agaaaggtga
     1441 aacccatgat ccccactgct tgatggggag ggtcaccctg cgcgaaaaat tgataggtga
     1501 gaggcagggt agtgggaaga ctgtaggcca aaaaagcggg gaaataggct gcatggacgg
     1561 ccgtcgagcc ggccgccatt cctcccagca ccagggctaa aaacatttgg tggcttggtg
     1621 aagaaggggg atacagtgca atgccggcca tgccccagat cactccggag gcaccattgc
     1681 cggcaacaaa ccaggccaac catgtacttg ggtgtaacag gggtttcgat gcgcggcgat
     1741 aggccaggac caaacctgac cagagaacgt taattcccac aataccgatg atccatcccc
     1801 acacgatctg ggtcggaatg acatccatga gaataaatgc gaccagccca caattaatga
     1861 tggaggccag aatggccatc gggactaagg aaaacagttg atgaatctgt tggcgacgaa
     1921 gttcgattaa ggagtgagaa gaggaagggg gtcctgagga tctcatgggg gtcggatccg
     1981 gccgagtgag catcaaggct tttccaaaat ctaaagagtc caggcataga cagtaccccg
     2041 gcatgtaccg ttcataccgt caacctgatg aagaagggat actcgcgacg gaactacccc
     2101 tccttctgca tatagcctgc ctttcatcaa ggactatgtc aagacgactg aggtgtcggg
     2161 ataaagaaag gaacatagga aaatcggata gggaggaagt cccaattggt gcaaagagcc
     2221 ttaatcggga tttccccggt tcgggctgat tgatgtctgt ctatgatacg atcaccatag
     2281 gatgaaatcg ttcacgcttc ttcagaaaac cattacggac tgtacgacct gtcagcgttt
     2341 gacgacctat cgtcaagcca ttgctaagga aaaggtcaaa cggtttcggg attgggaata
     2401 ttggggacgg cccatttccg ggttcggcga tcccaaggct caggtcttcg tggtgggctt
     2461 ggcccctgcg gcccatggag gaaaccgcac gggacgggtg tttaccggtg atcgaagcgg
     2521 agactggtta tatgaagcgc tgcatgcctt cgggtttgcc aaccagccgg attcgaccca
     2581 tcgggaggat gggctcacgc tgaacgattg ttatgtcgcg gcggtagtac gctgtgcccc
     2641 cccagccaat aaacccacaa aagccgaatt tgccgcctgc cgtccctatt tgcaggaaga
     2701 attacgatta ttgcggcggg tcaaagttgt tgtggcgctt gggaagatcg cctttgatga
     2761 atacttacgg acctgccaga ccatgggttg tcaggtgcct tctccccggc cgaaattcag
     2821 ccatggagcc caatatgcct tatcctgggg tgtgactttg gtcgggtctt atcatcccag
     2881 tcagcaaaat acctttacgg gtacactcac ccgtccgatg tttcacggga tttttagcac
     2941 cgtccgcaag ctgcttaccc catcccgacc tttgcgggct cctctgacat gatggtccga
     3001 ttaggccttg ttggagggtc tggcttccca atccgagaga aatttttgta aaccattatc
     3061 tgtcaacggg tgtttgacga gctgttgaaa tacgccaaag ggcatcgtgc aaatatgtcc
     3121 gccggtgaga gccgcttcga cgacatgttg cgggtggcga acgctggcga cgagaacctg
     3181 tgtggtgaaa ttatagtttt cgtagatcgt caggatttgt cgaatgagct ccatgccatt
     3241 ggaactgatg tcgtccaatc ggcctatgaa cggcgagacg caccatgccc ccgctttggc
     3301 ggccaataag gcttgagttg gagaaaaaca taatgtgacg ttgacccgga ttccttcgct
     3361 ggacaattgc ttcgtggcct tgagcccttc cggaataagt ggacatttca ccacgatatt
     3421 tttgtgaatt ttcgctaatt cttttccttc ctttaccatg gccgcagctt caacactgac
     3481 cacttcagcg ctgatgggcc catccacaat tccgcaaatt tccaggagga gatctttgaa
     3541 atcacgccct tcttttgaga caagcgaagg gttggtggtg atgccgtcta ccagaccata
     3601 actggccgct tcacggatct ctttgacgct tccggtatcg agatagagct tcatatcgaa
     3661 tccccttttt ttgtttgagc tggtgttgcc tgagcggtct cgtgtcccat tgtaatccac
     3721 gcccctgtct tgtacaaatt ttctttccat tggcgggaag aaaaattgat ctgataaacc
     3781 tgcctgacat gtcgtccccc atgaggtagt gtcatgaatc ttgaaggaca ggaccctcat
     3841 gcggtcctac cattcgagga tgaagaaaac ggagtcactc tttatttgat ggatcttgtc
     3901 gtttgactgg tagaggatcg aaggctagaa ttttcacaga gtagagagcg tgtccgggtt
     3961 tcagccattg ttggggaagg agacgggtgt gatctacaaa tatgtgctga taggactgtt
     4021 gggattggtg tgtgccggtt gtggagggaa tccgtatttg gatgcctccc ttgatcccgc
     4081 caaatttgaa ggaaaagaca aggcctggtt cgaagaaaac tggggaaagc ctagcgggaa
     4141 atccgctcga ttctttggag gagaaacctg ggtgtattca cgtatttccg gaggtgagtc
     4201 ccgattcccc tactttaatt tttccccgaa tcaatgtcac attaatttag attttgacaa
     4261 agaaggcaag ctggaagatt atgattatac ggattgctaa ggactctcgt gcccattcaa
     4321 tctgcttggg ttgaggagat cgttctgctg aatatgccat tccaaacggg ttttccatct
     4381 ttaggccgtc tgctcgtcct ttggacgtag gagtctggat tgtcccggac gattatcatt
     4441 acaccaacaa tcgaagagtt gtttgtggtt gccgccaagg aagtggtgca ggtggtgctg
     4501 gactgtcgga aagccggtcg cgattgtcgt gtggctttgg cgggtgggtc cacacctcga
     4561 ggattgtacc ggctcttgac gggtgccccc taccgaactc agatttcttg ggaccacctt
     4621 cgcgtttttt ggggagatga gcgcacagtc cctccggatc atcaagacag taatttccgc
     4681 atggccaaag aggcgttatt atctcatgtg ccaatcccct ccagacatgt atttcgaatt
     4741 gaaggagaag tacccgccca tgaagccgcg gcacggtatg aaaccgtcct gcaggaacaa
     4801 ttcaatctgt catcaggaga ggttccacaa ttcgacctga tcttattggg tatgggtccg
     4861 gatgggcata cggcgtcatt gtttcctgga acctcggctg tggcagagtc cgaaaaactg
     4921 gtgtcggcgc cgtgggtcga gaaattacag acacatcggg tgacgttgac cccaccggtc
     4981 cttaatgcgg cgaagcacgt ggtctttttg gtcagtggag tggataaagc gacggcgtta
     5041 caggtcgtct tggaagggcc ggcagatcct gcccgctatc ccgcccaagt cgtgaatcct
     5101 ccgacaggtc acatggtatg gttggtcaat caggatgcgg ctggcctttt gaaaagagaa
     5161 tcgatgacgt cacgtgtgtg aggaaggaga ggtcccatga aaatggttaa ggcgcccgag
     5221 gcatttattc acgccctcca taaaaaaccc atgacatgtc ccataccgga ttgttccggt
     5281 tccgtggctg tcgaggagcg ttcccagtcc accgatcgtg tgaaatcgtt tagattgcat
     5341 tgtgagcaat gtgattggca cgacacgata acgggtgatg aacaagtcga tccgccatgg
     5401 gacgaggggt ccctgatgga aattaccgag gagcatcttc tgcatttaga accggtttgt
     5461 ccctatgatc aggcgccggt ggattttcat tcgctgccga atccacgtcg tcgggctcgc
     5521 tatcgcatca cgtgtttttt ctgtgggcgg caggaggagt tggattggcc acctgaggaa
     5581 gcgaagcgat aatccttgga agtgtgccgg ggtctgagat ttcccttgag tgcccccttg
     5641 ccttattccc catgccctgt tattcctgtc agacccatct gttgtcgaac tcatgaaaaa
     5701 aattaaaaaa aagaagtcgg cttctcaagt cgtgccggct tttcttgttg ggtggactca
     5761 tggaaatccg cctccgccag gctatatcgc tgcctggttt gatgaggcgt atggcgggcc
     5821 attgcgcata cgtttcctca cctcgaacgg acaccaccat tttgaggcgg cgcacaccaa
     5881 ttggactgcc gaagtcaata ttgagccctc aacggcaatc gtggagcagt ggcagggtcg
     5941 cttacagtgg gaacagaccc aattggcaat cctgtttcct ccgaccaata cggcgaccga
     6001 tcgtcgggat gccctgctgc atgtggcgag aatggcgagg ggagtcactc tgctcaccga
     6061 cggaacgacc tatgacgtgg cgaccggcac atatctcaat ccttcggatt ggcgtgatcg
     6121 ggatttggag gtctttcgaa tcaccgatca cattcaggtg gaacatcgtg aagacgttca
     6181 gcgggcgcgc ctgtggttcc ataccagggg cctcacgaaa tttggtctgg atgaaatcga
     6241 gacctttcaa cccctgggcc tatcgagtcg agaggtgaaa gacaccatca taagggttgc
     6301 cgactctttg atcgagcagg gaaagaactc aaaggtgggg gaacgaatca ttcttgatgg
     6361 aagtggtccc caagtaatgg tggtgcgcca tcggaccgat cccgtgtatg ggactcctct
     6421 cgcattccgg gaatttatct tggagtaatg ggaccctaag cgcacaggaa gctgcaaccg
     6481 aacgcggtta ccgtggtgga gaaaccacgg taatgcgagt ctgcgcgcct cgtccaatcg
     6541 cttgatcgca ggggcttagg tgggtatcga tggcaatatt gggaatggag cccggcttgc
     6601 ttatcagttg ccgggtaagt gcctcgcccg atgccacagg gcgtggcagg cgatagagat
     6661 agatcgtgtc gatatcttct gcttgaaacc aggttgcgcc taaacttctc ttgtaaaatt
     6721 ccagaatttt ttcaggcgga tccgggctga ggagcacggc tgaaggaatg gtctggtagg
     6781 cgtgtccatg aatttgtgca ttcccgctgg gcatggcact ggccaggacg gcattcggat
     6841 atggagggac caagaaaaat tgtgccggag gcgtccagct attgagaaat gattcgtgca
     6901 tggtggtgag gcggcattgc gggtttttgc tcacggcgga aaaatcggcc aggggagcat
     6961 aggagcctgc atgggcgagt gtcgggaata cgaatagaaa tacgaggctc agcaaataag
     7021 acttcttcat gccttgactc cttgcatcgt ttgggattga gaaaattggc atgttgggca
     7081 tacaaaagtg agaatcatga cgtaagcatg cggtgcagtc aactgggagg gggcatagaa
     7141 atttggcggc ttgggttgtg aggcggaatg gaatttcatg aacctatcag ggaagctatg
     7201 tagcctcacg ataacgaatt gccccttgag gtgaggccga ccactgctgt ctgtgtttcg
     7261 ttatgaaagc tttggggcag acgctggttt ttgattaatg acattcatgg ccaaggcaat
     7321 catcgagccc acatccgaca cggaggctgg gagcacgaga gtgttactgg ctttcgccaa
     7381 ttctccaaat tctccaatat attgttccgc cacacgaagc tgaaccgcct caaatccgcc
     7441 ggggagttga atcgcttcgg caacctttcg aataccttcg gacgtggcgg tcgcaatcgc
     7501 gagaatggct gacgcttctc cttcggcttc gttgatctgt tgctgtcgtt tcgcttcaga
     7561 tgctttgatg acctgctgct tgtccccctc ggcttcgtta atggccgcgt cccgttgacc
     7621 ttcggatgcc agcaccactg cgcgtttttc ccgttccgcc cgcatttgct tttccatcgc
     7681 gctgagcaca tccttgggcg ggttgatatt acggatttcg tatcgaagga ctttggcacc
     7741 ccagggagag gaggctttgt ccaattcgtt cacgacggaa atgttgatcg tggtccgttc
     7801 ttcgaaggtc ttatccaggt caatttttcc gatctcacta cgaagcgtag tttgggcgag
     7861 ctgagaaatg gcgaaccggt aatcggtaat gccataggac gcgcgttcgg catctaggac
     7921 tttgatatac aagaccccgt ccactccgac ctgcacgtta tcccgggtaa tacagatttg
     7981 ttcgggaacg tccacggcca tttcttttag ggtatgtttg tatcgaatca catctaaaaa
     8041 cgggataagg atgtgaagtc cggcatttaa ggttgacgaa tactttccta accgttcaac
     8101 gacataggcg ctttgttgag gaaccacgat ggccgttttt gacagaataa taaacgccag
     8161 taaggcgagg acaagcatga ccagtaattc acccgacatg agattcctcc tgtgtcatcg
     8221 gatccgcctg aatccagagt gtcaaccctt caactcggat aactttggcc tgggatcctt
     8281 tgcctagggt tgatgtgccg gcattgcggg cagtccagct tgttccatgg agttccacct
     8341 tccctattcg ttgggcgtca agattttcca aaacagtggc aaactctcca accatggaat
     8401 ccaccggaac ctcatccttt tcccttatga agcctgttcc ttgaaggggt ttcttcatgg
     8461 cgaggagaga gaggataccg aagagcacga aacacaacca ttgcagccag gcggcttcgg
     8521 tcaagcttaa ccaggtgagg gcactcacca tcaacgcggc taccccgaaa aacagaaaat
     8581 aaaaattgcc gaggcctccc agggtgagga tttccagccc tattaagacc aaacccatta
     8641 tcgaccaatg ccaccaaagc atcgcgaagg gaacctctct gttgcaagga taacggcaga
     8701 ggggaccagg gggatccgag ctattgccgt ttttctatta tatcgggcgt
//
LOCUS       DATIAL010000174        12320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_784936, whole genome shotgun sequence.
ACCESSION   DATIAL010000174 DATIAL010000000
VERSION     DATIAL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12320
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784936"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VLA60_05355"
     CDS             <1..776
                     /locus_tag="VLA60_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HSF08818.1"
                     /translation="LINIWGADHHGYIPRMEATVQAFGFAKEALRIVLVQMVSLRRGG
                     QKIEMSKRAGEFVTLREVIDEVGPDAAKFFFLMRRADTHLDFDLELAKQQSSDNPVYY
                     VQYAHARLASLFRVAQERQVPLPTVQDVDQALLIQDEELGLIKTLAQYPFVVENSAMA
                     LEPHRVTFYLQELAAQLHAYYNKNRVLPSLDPERNTGDTGDSIKLGASEHTTRLSQER
                     IYESISPDLTAARLALLRQVQTVIGNGLAILGISAPEKM"
     gene            808..1983
                     /gene="tgt"
                     /locus_tag="VLA60_05360"
     CDS             808..1983
                     /gene="tgt"
                     /locus_tag="VLA60_05360"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSF08819.1"
                     /translation="MSKEPSTSQSGPNRFHIQHTLPSGRARIGRLLTSHGEIDTPAFM
                     PVGSQGTVKGLDPNEVRECGFHMVLANAYHLFLRPGHELIESLGGLHAFMQWPGAILT
                     DSGGYQMVSLADLCEVTEEGVGFRSHIDGAWHLLSPEKSMAIQVALGSDIMMVLDHCP
                     TFPCTEQKAREAMQRTTRWAQRCAAVPRKEHQWLFGIVQGGVFVQLRQESTQELVNLN
                     MDGYALGGLSLGEEKAAMLAMIETVIDQLPPGRPRYLMGVGLPEDIVEGVARGLDLFD
                     CVIPTRHGRTGWLFTSTGKVLIKQARYARDSNPIDGECGCPVCQRFSRAYLRHLFLSH
                     EMLGVRLNTIHNLWYYGSLMKGLRQAISANQFDDYRKDFYRRRECDTSETLIQEVST"
     gene            2025..2348
                     /gene="yajC"
                     /locus_tag="VLA60_05365"
     CDS             2025..2348
                     /gene="yajC"
                     /locus_tag="VLA60_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSF08820.1"
                     /translation="MNLDSYAWAQAGGAGPDSSAGLLSLIPFLLIFVVFYFLLILPQQ
                     RRQKKQRELLDSLKKGDKVITSSGIWGTVTNLDKETATLQVADNTKIRLQRDHIASIR
                     TSNES"
     gene            2368..4002
                     /gene="secD"
                     /locus_tag="VLA60_05370"
     CDS             2368..4002
                     /gene="secD"
                     /locus_tag="VLA60_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSF08821.1"
                     /translation="MKKLRGRLFLLFVVTVVSVILALPSFPGLFQSLPDGVKRVLSHR
                     GISLGLDLQGGIHLVLEVEEERAVEIAVDRIRKAVEDLLKDKAIVVEGVRREGSKMIV
                     ITLQQEADGEKVRTLLDEAFPNFESQNPSGTRLVYELRSTEVERVKTSAINQALETLR
                     NRIDEFGVAEPLIQRLGLNQIAIQLPGVKDPQRAKDLIQETALLEFKLLEESKAALDL
                     PPQVEKGQEDSVRKTLEGKIPEGAEILFETAISEPDGRAYSIPYLVKKDAVLIGDVLQ
                     DARVTIGDFNEPIVSITFDSKGAREFDELTAANIGKRMAVVLDGKVYSAPVIRDRISG
                     GRAIIEGTFTTAEANDLAVVLRAGALPAPLKTLQDLTVGPSLGQDSIEKGLRTTIIAG
                     TLVLIFMIVYYRLSGLIANMAVFLNLICLLGALSGLNATLTLPGIAGIILTIGMGVDS
                     NVLIFERIREELRQGRPVRLAVDSGYNKAFLTIVDSHVTTLITGLALFLFGTGPIKGF
                     AVTLCLGIAINLFTALVGTKVVFDFLNRRKLDSLSI"
     gene            4156..5076
                     /gene="secF"
                     /locus_tag="VLA60_05375"
     CDS             4156..5076
                     /gene="secF"
                     /locus_tag="VLA60_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HSF08822.1"
                     /translation="MMEILGKTDIDFMGKRNIAFAVSGILASLGLVAVIGILFGWANL
                     GIDFAGGTAVQLKFDKPLLIAEARKALDAHGLGSAELQEFPQDQKLLIRVKTETTIEE
                     GISENIIQAFQAEFPDHGFVVDSSTSIGPTIGKKLQEDALIAVILSLAGIILYVAIRF
                     EFRFGVAAAIATFHDVLAVLGIYFLLNTEITLLVVTALLTLAGYSLTDTVIVFDRIRE
                     NLRQRRRETTTTIINNGINQVLSRTLITTLTVVLVLVPLTIWGGEVLHDFSLALLLGV
                     MVGTYSSIFVASPLLLLWPGGEGKLLSRGK"
     gene            5179..8364
                     /locus_tag="VLA60_05380"
     CDS             5179..8364
                     /locus_tag="VLA60_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF08823.1"
                     /translation="MKRFGFPLQLKGKTILAMVLVGLLPLILSLLLTYFEEKRALREA
                     AGITMKGIAVEVARKVETQIVRGINEAQQLATIPFIRSAIMNSNRSYADKNPDEIQAL
                     IQQWQESWRAQSNKDEFPVFLNKYATDYLIQWHAIRKSDYLAIVVVDTQGALVLSSFP
                     QVNFFHGNSLWWEAVVQHHDAQAFVSDLSFDPGFGTHVLNVGVPIWDDHRQNVVGAIS
                     ILLRRDSLFRSISEVAAGKTGHAMLTASDGMPILCPAFSLEEHVMPPNVVSAFQQGGV
                     GWLVADPDSHGMPNAIVGYAPLHLGMPLAPESFGGKSWHMLTSQDSAETYAPLDQLLS
                     KVIFYGISVFVILWGAGVVVAGRIVKPIQALSQGVEQFGGGNLSEKIAIRTGDEIERL
                     ADTFNAMADNLQHSFSELNQKVDEIGRLEQKYRDLIENAPEMIHQLDPAGRFVHVNTT
                     ELQKLGYSLADMLEMSLWDIVPAEQQEEVRAYVQGLALGGARSIETVFRTQSREVMDV
                     EIHSTTLMDPGTHSVVYSRGFVRDITARKALEREVERYTTQLEGIVAERTQQLSDSEA
                     GYKALFNLAADSIFVVDSEGRILDVNAREREILGYVPSDLEGAYFVKLVPPAFQMISQ
                     NLLERVSGEESKVPTTEIEVYDRQGIMKSMEMDLVRIDMGGRASIMVQLRDITEHKKL
                     EAELQRYNEVLEEKVSERTREIEQAKLYIESLLENANDVIYTLDVDLRFTYVNGKVDA
                     WGYRKEDLIGKPYLSLLSKRYRGRYLKETLDLGTKQVYEVEVVSREGELRSVLVSVAP
                     LLNDEGVYTGVLGIARDITERKHLERQVQNSERLASIGKLAAGVAHEINNPLGGILNC
                     LYNIRKGTLTPERSQEYLHFMEDGLRRVQRIVRQLLDFSQQREPELAMTDLHQILDRV
                     LVLTEHVFLVKHATLSKDYDETLPMVMVDAHMIEQVIMNLVLNAVQALKEGGQVTLRT
                     RKQEEGYEIEVEDTGCGIPQEIRPHIFDPFFTTKGTGEGTGLGLSVSLGIVQRHAGEM
                     LVESEEGKGTRFTIRLPLVRSRAGIPVKVGT"
     gene            8361..9821
                     /locus_tag="VLA60_05385"
     CDS             8361..9821
                     /locus_tag="VLA60_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF08824.1"
                     /translation="MTHGSVLIIDDEPLMRVSMVDALKAIGYVVQEASSGLEGLERLR
                     TSRFNLVITDLRLPGVDGLHIVKQCREQCPGTEVIVITAHGSVDTAVDAMKSGAYDYI
                     TKPFSMDELLLSVQRVCAVVALREENRVLRDALEKKFSFQGIVGKNERMRQVLEKVKL
                     VSATDATVLVVGESGTGKELIANAIHTNSARRNHALVKVSCAALPETLLEAELFGHEK
                     GAFTGALRQRQGRFELAHQGTLFLDEIGEISPLVQIKLLRVLQERQFERVGGNDTIEV
                     DVRLVCATQKDLRKEVEQGRFREDLYYRLNVVPVQLPPLRERREDIFMIAQHFLETMS
                     GRNHQEPKSLSRQAREVLLQYSFPGNVRELENTIERALALAQHTQEIQVWDLCGQSRC
                     PYAGGEPQKGCGFCGNQEGRRGVKNGAMETLAEAREHFERGHILEILNRTGWSRMTAS
                     KVLGLSRKGLWEKCKRYGIVRACDDTDSEKGDEDDG"
     gene            complement(9896..10990)
                     /locus_tag="VLA60_05390"
     CDS             complement(9896..10990)
                     /locus_tag="VLA60_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HSF08825.1"
                     /translation="MTQPTSPDTLSSNPGFIQNPDSQPPPLIDSLAYWKGGARELKSF
                     RGHTQEIWAVAFSPDGLTLASGGNDRFIRIWDIETGRLLRSLRGHTHVVREVKYSPDG
                     QIIASASEDRTIRLWNPQTGESLRLLFTRYDHAVCSISFSPDGLMLARAGQNKDIKIW
                     EVTTGTELMTLLGKDQYDHNWSVCTAFSPDGIHLVAGTDIGKIKLYEVLPSGEEKIVH
                     NGHWRDEADDEETENRGFYVDDQGGFQKPMEYWIGALTFTPDGSTMISGSRDCTIKFW
                     DMPTMEERRTLKGHSEWVRNLLVTQDGQVLISASDDGTVKMWDIQTGRQFRTLRSHKG
                     PVRGIALSSDGKFLATAGNDRIITLWDGGE"
     gene            11245..12243
                     /locus_tag="VLA60_05395"
     CDS             11245..12243
                     /locus_tag="VLA60_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08826.1"
                     /translation="MEIRFQPALLQEVIDSFAEKTEREGDPTYFNEFHEFADPIYEKF
                     SLDDRDPEFKRLYQHLFAKWGFAEILRDAFDDFPVLRDKTGIVLVRGVLKEDQEGVDV
                     LRKWGVVEEKLARQFEEAGKKGVGIKLIPRRFYDPAITRYLRHELTHISDMLDEAFGY
                     DPDTKVGLNPGEEHLILNRYRVLWNLHVDSRIARTGKEPMFSKEYRFREFRSWYRKIP
                     PGQVESVFEGIWQTDYLTHAELVEMASDTIRVIERAIEVEDSEVPDVPTKPMLLPGFP
                     CPLCRFPTYTWVENMDETLEGFVLDFIRENHPGWDVEYGACDRCVEVYKLRASGVV"
BASE COUNT         3004 a         2552 c         3466 g         3298 t
ORIGIN      
        1 ccttaataaa tatttggggt gcggaccatc atgggtatat tccacgcatg gaagctacgg
       61 ttcaagcgtt tggctttgcc aaggaggctt tgcgaatcgt cctggtgcaa atggtgagct
      121 tgcgacgtgg tggtcaaaaa attgagatgt caaagcgagc cggtgaattt gtgacccttc
      181 gcgaggtgat tgatgaggtg ggtcccgatg ccgccaaatt ctttttcctg atgagaaggg
      241 ccgatacgca tttggatttt gatctggaat tggccaaaca acagtcttcc gacaatcccg
      301 tctattatgt tcagtatgcc catgcgcgat tggccagtct ctttcgagtg gcgcaggaac
      361 gccaagtacc ccttccgact gtccaggatg tcgatcaggc cttgttgatc caggatgaag
      421 agttagggct gattaaaacg ttggcacagt atccttttgt ggtcgaaaac agtgcaatgg
      481 cgttagagcc ccatcgggtc acattttatc tccaagagct tgcggcccaa ttgcatgcct
      541 actacaacaa aaatcgggtc cttccctctt tggatcctga gaggaacacc ggggatacgg
      601 gagacagcat taaactgggg gcaagtgagc atactacacg gttgagtcag gagcgcatct
      661 atgaaagtat ttcgccggac ttgaccgctg ctcgcttagc cctgcttcgg caagtccaga
      721 cggtcattgg taatgggctg gccatcctgg gaatttccgc gccggagaaa atgtaacgtg
      781 catgaaggtt aagcatacgc agaaataatg tccaaagaac catccacttc acaatcaggt
      841 cccaatcgtt ttcatattca gcatacgttg ccttctggtc gtgctaggat tggacgactt
      901 ttaacctccc atggtgagat cgatactcct gcctttatgc cggttggctc acaggggacg
      961 gtcaaaggac tggacccgaa tgaagtccgc gaatgcgggt ttcatatggt tttggcgaat
     1021 gcctatcatc tatttttgcg acctgggcat gaactcattg aatctctggg agggcttcat
     1081 gctttcatgc aatggcccgg agccattttg accgatagtg gtggctacca gatggtgagc
     1141 ctggccgatc tttgtgaggt gaccgaggag ggggttggct ttcgttctca tatcgacggt
     1201 gcgtggcatt tactctcacc ggaaaaaagt atggccatcc aggtcgcact tggctccgat
     1261 attatgatgg ttcttgacca ttgtccgaca tttccttgta ccgaacagaa agcacgtgag
     1321 gctatgcagc gaacgaccag gtgggctcag cgatgcgctg cggtaccgag aaaagaacat
     1381 cagtggttgt ttgggattgt tcagggcgga gtctttgtcc aattgaggca agagtcgact
     1441 caggagttgg tcaatttgaa tatggatggc tatgccttgg gtggattatc cttgggagaa
     1501 gaaaaggccg caatgttggc gatgatcgag acggtgattg accaacttcc acccggtcgt
     1561 ccccgctact taatgggagt tgggcttcct gaggatattg tggaaggggt ggctcggggg
     1621 ttggatttat ttgattgcgt tattccgacc aggcatgggc gcaccggttg gctgtttacc
     1681 tcgacaggga aggtgttgat taaacaggct cgctatgccc gtgattccaa tcccattgat
     1741 ggcgaatgtg ggtgtccggt ctgccaacgg ttttccagag cctatttgcg gcacttgttt
     1801 ttatcgcatg aaatgttagg cgtacgtctg aatacgatcc ataatctttg gtactatggc
     1861 tcgctcatga aggggctacg acaagccatt agcgcgaatc agtttgacga ctaccggaaa
     1921 gacttttacc gccgacgcga gtgtgacacg agtgaaacgc tcattcagga agtatcgacc
     1981 tgatcgaatg ggttggccac agacacattt aaattgaggt acttatgaac ctagattcct
     2041 atgcttgggc tcaagccggg ggagccggtc ccgattcctc cgccggctta ttatcgctca
     2101 ttccttttct tctgattttt gtggtgtttt attttttgct aattttgccg caacaacgtc
     2161 ggcaaaaaaa acaacgggaa ttgttggata gcctaaaaaa aggcgataag gtcattacgt
     2221 cttcgggaat ttggggaacc gtgaccaatt tggataagga aacggctacc ctacaagtcg
     2281 cggataatac aaaaattcgg ttgcaacgcg atcatatcgc gagcatccgt acgtcgaatg
     2341 aatcatgatg gatcaagggg tatatgaatg aaaaagcttc gtgggaggtt gtttctcctt
     2401 tttgtggtca cggtggtgtc cgtcatcctg gccttgcctt cgttcccagg gctgttccag
     2461 tctttgcctg atggggttaa gcgggtatta agtcaccgtg ggatttcgtt gggtctcgat
     2521 cttcagggag gcattcacct ggttttggaa gtcgaagagg aacgagcggt cgagattgcc
     2581 gttgatcgaa ttcgtaaggc tgttgaggat ctgctgaagg acaaggcgat cgtggtggag
     2641 ggtgttcgtc gtgaggggtc gaagatgatt gtcatcaccc ttcaacaaga agccgatggg
     2701 gagaaagttc gaacgctgct cgatgaggct ttcccgaact ttgagtcgca aaatccgtca
     2761 ggaacgcgtt tggtatacga acttcgctct accgaggtgg agcgagtgaa aacctcagcc
     2821 atcaaccagg cacttgaaac cctcagaaac cggattgatg aatttggcgt cgccgaacct
     2881 cttatccaac gattggggct caaccaaatt gcgattcaat tgcctggggt caaggaccct
     2941 caacgggcta aagatctcat tcaagaaacg gctctcttag aatttaaatt gttggaggag
     3001 tccaaggccg cattggattt accgcctcaa gtcgaaaaag gccaagagga ttcggtgagg
     3061 aagaccttag agggtaaaat tcctgaaggg gcagaaattc tctttgaaac ggcgatctcg
     3121 gaaccggatg gtcgggcata tagtattccc taccttgtga aaaaagatgc cgttctcatc
     3181 ggagatgtgt tgcaggatgc gcgcgtgacg attggggatt tcaatgagcc gatcgtcagt
     3241 attacctttg acagcaaggg ggcacgagag tttgatgagc tcaccgctgc caacattggt
     3301 aaacggatgg cggtggtcct ggatgggaag gtgtattcgg cccccgttat tcgagatcgc
     3361 attagcggcg gccgggcgat cattgaaggc acctttacca ctgcagaagc gaatgatctg
     3421 gctgtggtgc tccgggctgg tgcgttaccg gcaccgttga aaaccttgca ggatttaacc
     3481 gttggacctt ctctgggaca ggattcaatt gagaaggggt tacgaacaac gattattgcc
     3541 gggacgctgg tcctcatttt tatgattgtg tattaccggc tctccggtct catcgccaat
     3601 atggctgtgt ttctcaattt aatctgtttg ctgggagctt tatcagggct taatgccacc
     3661 ttaactttac caggcattgc tggaattatt ttgaccattg gaatgggagt cgactccaat
     3721 gtgttgatat ttgagcgaat tagagaggag cttcgccagg gccgtcccgt tcgtttagcc
     3781 gtagatagtg gctataataa agcctttttg acgatcgtag attcccatgt gacgaccctg
     3841 atcacggggt tggcgctgtt tttatttgga acgggaccta tcaaagggtt tgcggtaacc
     3901 ttgtgcttgg gaattgcgat taacttgttt acggcgttgg tggggacgaa agtggtgttt
     3961 gatttcctga accgacgaaa gttggactcc ctcagtatct aagggggtca gccttgctga
     4021 gaggtggttg gtcgaacctt agtggttgag gggaggcaag gcgcagcatt ttcaaatacg
     4081 aggatcgatt gagaaagggc gttatcacaa gctcatagac aggggatatt ctttggggag
     4141 gagaaatttt tggtcatgat ggaaattctt gggaaaacag atattgattt tatgggaaag
     4201 cggaacattg ctttcgcggt atcgggcatt ctggcttctc tgggattggt ggccgttatt
     4261 ggaattctgt ttggatgggc aaacctggga atcgattttg ccggggggac ggcggttcag
     4321 cttaaatttg ataaaccact cctgattgct gaagccagaa aggccttgga tgcccatgga
     4381 ttgggttccg ctgaacttca ggagtttccc caagatcaga agctcttgat tcgtgtaaaa
     4441 accgaaacca cgatcgaaga aggaatcagt gagaatatca ttcaggcttt tcaagctgaa
     4501 tttcctgatc atggctttgt agtggattcc agtacttcaa taggccccac aattggaaag
     4561 aaactgcaag aagatgcctt gattgcagtc atactttccc tggcaggcat tatcctctat
     4621 gtcgctattc gttttgagtt tcgttttggc gtggcggcag ccattgccac atttcatgac
     4681 gttctggcgg tgttgggcat ttattttctg ttgaatacag aaattacgct gctggtggtg
     4741 acggcgctat tgaccctggc agggtattct ttaaccgata ccgtcatcgt gtttgaccgg
     4801 attcgggaaa atcttcgtca gaggagacgg gaaacgacga cgacgattat taataacgga
     4861 atcaatcagg tcttaagcag aacattaatt acgacactga cggtggttct ggtcttggtg
     4921 ccgctgacca tatggggagg ggaagtctta catgattttt cattggcctt actcttggga
     4981 gtcatggtgg ggacctattc ttcaattttt gtcgcgagtc ccctcctctt actgtggcct
     5041 ggtggagagg gaaaactact aagccgaggt aaatagtctt ccttgttacc gtttcttgac
     5101 atcaaggaaa aaggagtacc aaaatgtaga aaatcgtagg agttagacgt tatttatgga
     5161 ttttgcgatg ttaccaaagt gaaacgcttt gggtttcccc tccaactaaa gggcaagacg
     5221 attctggcga tggtcctggt tggtcttctg ccccttattc tttctctcct cctcacatat
     5281 tttgaagaaa aacgtgcact aagggaagcc gccggtatca ccatgaaggg gattgcggtg
     5341 gaagtggctc gtaaagtcga gacccagatt gttcgtggaa taaatgaggc gcaacagctg
     5401 gccacaattc cctttattcg aagtgccatc atgaattcga accggagtta tgctgataaa
     5461 aatccagacg aaattcaggc tctaattcag cagtggcagg aaagttggcg ggcccaatcc
     5521 aataaggatg agttcccggt ttttctgaac aaatatgcta ccgactacct gattcaatgg
     5581 catgcgattc gaaaatcgga ttatctcgcg attgttgtgg ttgatacgca gggggcattg
     5641 gtgttgagct cttttccaca agtcaatttt ttccatggaa acagtctctg gtgggaggcc
     5701 gtggttcaac atcatgatgc ccaggcgttt gtgagcgatt tgtcatttga tccagggttt
     5761 gggactcatg tcctgaatgt tggcgtcccg atttgggatg atcatcgaca aaacgtcgtg
     5821 ggagctatca gtattcttct gagaagggat tcgttatttc ggtcgatttc tgaagtggcc
     5881 gccgggaaaa cgggtcatgc catgttaacc gcatccgatg gaatgccgat tctctgcccc
     5941 gcgttttctt tggaagagca tgtgatgccg cctaatgtgg tcagtgcgtt tcaacaaggt
     6001 ggtgttgggt ggttggttgc cgatccggat tcacatggta tgccgaatgc cattgttggc
     6061 tatgctcctc ttcacttggg gatgccgcta gctccagaaa gttttggtgg gaagtcctgg
     6121 catatgttga ccagtcaaga ttctgccgag acgtatgcac cgctggatca attattaagt
     6181 aaggtcatct tctatggcat ctctgttttt gtcatcttgt ggggggcggg agtcgtggtt
     6241 gctggccgga tcgtgaagcc catacaggcc ttatcgcagg gagttgagca atttggaggt
     6301 gggaatcttt cggaaaaaat tgcgatccgt accggtgatg aaatcgaacg cttggctgat
     6361 accttcaatg ccatggcgga caatctacag cattcgtttt ctgagttgaa tcagaaagtg
     6421 gatgaaattg ggcgattgga gcagaaatat cgtgatctca ttgaaaatgc tccggaaatg
     6481 attcatcaat tagatcccgc agggcggttc gtccatgtaa atacgactga gttgcaaaag
     6541 ctgggatatt cacttgccga catgcttgag atgtcgctct gggatattgt cccggcggaa
     6601 caacaagagg aggtgcgggc ctatgtccaa gggctggcct tgggtggggc gcgctccata
     6661 gaaactgttt ttcgtacgca atcacgtgaa gtgatggatg tggaaattca ttcaacaacg
     6721 ctgatggatc cggggaccca ttcggtggtg tattcgaggg gatttgtgag ggatatcact
     6781 gcccgaaagg ctctggagcg tgaagttgag cgatatacca cccaattgga agggattgtc
     6841 gccgaacgaa cccaacaact gtctgactca gaggcgggct ataaagcgtt atttaatttg
     6901 gctgccgatt caatctttgt ggttgattcg gaaggccgta tcttggatgt caatgcgcgt
     6961 gaacgggaaa ttctagggta tgtcccttct gatttagaag gcgcgtactt cgtgaagctt
     7021 gttcctccgg cattccagat gatcagtcag aatttgctgg aacgagtcag tggtgaagag
     7081 tcaaaggtgc ccacgacgga gattgaagtt tatgaccggc aaggcatcat gaagtccatg
     7141 gagatggacc tggttcgcat tgatatgggt ggaagagcct caatcatggt gcagttgcga
     7201 gatattaccg agcataaaaa gcttgaagct gaactgcaac gatacaatga agtcctggaa
     7261 gagaaagtct ctgagcgtac gcgggaaatt gagcaagcca agctgtacat tgaaagtttg
     7321 ttggaaaatg cgaatgacgt gatttatacc ttggacgttg atttacggtt tacttatgtc
     7381 aatgggaaag ttgatgcatg gggatatcga aaagaagact tgattggtaa accatatctc
     7441 tcgttactct ccaagcggta tcgtggacgg tatttgaaag aaacattgga tcttgggaca
     7501 aagcaagtct acgaagtaga ggtggtcagt cgagagggag agttgcggtc ggttctggtg
     7561 agtgtggccc cgcttctcaa tgatgaaggc gtttacaccg gagtattggg tattgccagg
     7621 gatattacgg aacggaagca tttggaacgc caggtgcaga attctgaacg tttagcctcc
     7681 attggtaagc tggccgccgg ggttgcacat gaaattaaca atccgttagg gggaatttta
     7741 aattgtttat ataatattcg aaagggaacg ttgacgcctg agcgatctca ggaatatttg
     7801 cattttatgg aggatggatt gcgacgcgtt caacgcatcg ttcggcagct cctggatttt
     7861 tcccagcaac gtgaaccgga attagccatg acagatcttc atcaaatttt agaccgagtt
     7921 ctggttttga ccgagcatgt attcctggtc aaacatgcca ctctgagtaa ggattatgat
     7981 gagacactgc cgatggtgat ggtcgatgcg catatgattg agcaggtaat tatgaactta
     8041 gtcctgaatg cggttcaagc actcaaagag ggtggccagg tgaccttgcg tacccgaaag
     8101 caggaagaag gatatgaaat tgaagtggag gatactgggt gcggaatacc ccaagagatt
     8161 cgacctcata tttttgatcc cttttttacg acgaaaggga ccggagaggg gaccgggttg
     8221 ggattgtcgg taagtttggg aattgtgcag cggcacgcag gtgaaatgtt ggttgagagt
     8281 gaagagggaa aaggcactcg ttttaccatt cgcttgccct tggttcgatc tcgtgcgggc
     8341 attcctgtaa aggtaggcac atgactcacg gttccgtttt gataattgat gatgagccct
     8401 tgatgcgagt ctctatggtg gatgcgctga aggctatcgg ctatgtggta caggaagcca
     8461 gctccgggtt ggagggactc gagcggttgc ggacttcacg gtttaatttg gtcattactg
     8521 atttgcgatt gcccggtgtg gatggactgc atatcgtaaa gcagtgccgg gagcagtgcc
     8581 ctggcacgga agtaattgtc attacggcac atggttccgt tgacacggcg gtcgatgcga
     8641 tgaaaagcgg tgcctacgac tacatcacga aacccttttc tatggatgaa ttgttattga
     8701 gtgtgcagcg ggtttgtgcg gtggtggctc ttcgtgaaga gaatcgggtg ttacgggatg
     8761 ccttggaaaa gaaatttagt ttccaaggga ttgtgggaaa aaatgagcgg atgcgacaag
     8821 tgctggagaa agtcaagtta gtctcggcca ctgatgcgac cgttttagtg gtaggggaaa
     8881 gtggaacggg aaaggaattg attgccaatg ccatacatac caatagcgct cgccgaaatc
     8941 atgccttggt caaagtaagt tgtgccgcgc taccggaaac gctgttggag gcggaactgt
     9001 ttgggcatga aaaaggggcg tttacgggag ccctccggca acggcagggg cggttcgaac
     9061 ttgctcatca gggaacgctt tttttggatg agatcggcga aatttccccc ttggtccaga
     9121 ttaaattgct tcgtgttctc caggaacggc aatttgaacg cgtcggggga aatgacacga
     9181 ttgaagtgga tgtgcgatta gtctgtgcaa cgcagaagga tttgcggaaa gaagttgaac
     9241 aaggacgatt ccgggaagat ctctattatc gattaaatgt ggtgccagtt cagcttcccc
     9301 ctctccgaga acgtcgagaa gatattttca tgatcgccca gcattttttg gagacgatgt
     9361 caggtcgaaa ccaccaagaa cccaaatctt tatcacggca agctcgcgag gtgttattgc
     9421 agtattcctt tccggggaat gtccgtgaat tggaaaatac tattgaacgg gcgttagcat
     9481 tggcacaaca cactcaggaa atccaggttt gggatctctg cggacagagt cgatgtccat
     9541 atgcgggagg ggaaccgcaa aaaggatgcg ggttttgcgg gaatcaggaa ggcaggagag
     9601 gcgtgaaaaa tggagcgatg gaaacattgg cggaagcccg ggagcatttt gaacgggggc
     9661 atattcttga aatactcaat cgaacagggt ggagtcggat gacggcctcg aaggttttag
     9721 gattatcgag aaaagggctt tgggaaaaat gtaaacggta tggaatcgtg cgggcatgcg
     9781 acgacacgga ctctgagaag ggtgatgagg atgacggttg aaagggcagc cctctttaga
     9841 aagagagaac tgccctgagt attcgaagct tagggattct gtgacgggac ttagattatt
     9901 ctccaccatc ccagagggtg atgatccggt catttccggc tgtagcgagg aatttcccat
     9961 cagaagaaag agcgattccc ctgacaggtc ctttgtgtga ccgaagcgtt cggaattgac
    10021 ggcctgtttg gatgtcccac attttgacgg tcccgtcatc gctggcgctg ataagcacct
    10081 ggccatcctg ggtcacaaga agatttctga cccattcaga gtgacctttc agcgtgcggc
    10141 gttcttccat cgtcggcatg tcccagaatt taatcgtaca gtcgcggctt cctgagatca
    10201 tggtgctacc gtcaggggta aaggtgaggg ccccaatcca atattccatg ggtttttgga
    10261 atccaccttg atcgtctacg taaaaaccac gattttcggt ctcttcgtca tcggcctcat
    10321 ctctccaatg gccgttgtgt acaatttttt cttccccgct tggcaaaacc tcatagagct
    10381 taattttccc gatatcggtg ccagccacaa gatgaatgcc gtcaggtgaa aatgctgtgc
    10441 agacactcca attatggtcg tattgatcct taccaagcag ggtcattagt tcggtgcctg
    10501 tggtcacttc ccaaattttg atgtctttat tctgccccgc cctggcgagc atgagcccgt
    10561 ctggggaaaa ggaaatactg catacggcat ggtcgtaacg agtaaaaagt aaccgaaggg
    10621 attctccggt ttgaggattc cacagccgaa tggtccggtc ctcacttgcg gaagcgatta
    10681 tttggccatc agggctatac ttgacttctc gaacgacatg tgtgtgccct cgtagggaac
    10741 gaagaaggcg gccggtctca atatcccaaa tccggataaa acgatcattc cctccgcttg
    10801 ccaatgtcag cccatccggg gaaaaggcga cagcccaaat ctcttgtgtg tgaccacgga
    10861 atgacttcag ttccctggca ccgcctttcc aataggccag tgaatcgatg agtggcggag
    10921 gttgcgaatc aggattttgg atgaacccgg gattagaact gagtgtgtct gggcttgtgg
    10981 gctgggtcat ggaattcctc gcttcggaaa cagattgaca tcaaagggat cggttaaacg
    11041 gattcaggaa tggatgaatg ggattctctt gccaaaaggg aaaacgcatt cctataattc
    11101 aactagttat gatactaaac ggcgcttttc gagggagtca aggtagctac ctcccaaatt
    11161 attgtgaatt tattattacg gtgatcatct tccgagaaat aaaactcgtc ttggggtgat
    11221 cttgcggtaa acggaggttt gctcatggaa attcgttttc aacccgccct ccttcaggag
    11281 gtgattgatt cgtttgctga aaaaactgag cgtgaaggtg acccgactta ttttaatgaa
    11341 tttcatgagt ttgccgatcc catttacgag aagttttccc ttgatgaccg tgatccggag
    11401 ttcaaacgcc tctatcaaca tttatttgct aaatgggggt ttgccgaaat tcttcgtgat
    11461 gcttttgatg acttcccggt tttgcgagat aagactggaa ttgtgttggt gcgaggcgtt
    11521 ttgaaagaag atcaggaagg ggttgatgtt cttcggaagt ggggagtggt cgaagaaaaa
    11581 ctcgctcgcc aatttgaaga ggccgggaaa aaaggggtgg ggattaaatt gatccctcgg
    11641 cgattttatg atccagccat tacccgttac ctgcgacatg agttaacgca tatttctgac
    11701 atgttggatg aggcttttgg ttacgatcct gacacaaaag tggggttgaa ccctggggaa
    11761 gaacatttga ttttaaatcg ttaccgtgtg ctgtggaacc ttcatgtcga tagccggatt
    11821 gcccgaacag gcaaagagcc gatgttttcc aaggaatatc ggtttcgtga gttccgttct
    11881 tggtatcgaa aaattcctcc cggtcaggtg gagtccgtat ttgaagggat ctggcagacg
    11941 gattatctta ctcatgccga attagtggaa atggcttcag atacgatacg agtgattgag
    12001 agagccattg aggttgagga cagtgaggtg ccggatgtgc caacgaaacc catgctcctc
    12061 ccagggtttc cgtgtcctct ttgtcgattc ccaacttata cctgggtaga aaacatggat
    12121 gagacgctcg aaggttttgt cttagatttt attcgcgaaa accacccagg atgggatgtg
    12181 gaatatggag cctgtgacag atgcgtggag gtgtataagc tgcgggcctc cggagtggta
    12241 taaattcaaa aaacaccttt cctcgtgtcc cattggtttt attggtttat agactattga
    12301 aagcgtcgta atgtcggact
//
LOCUS       DATIAL010000175         8798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_462511, whole genome shotgun sequence.
ACCESSION   DATIAL010000175 DATIAL010000000
VERSION     DATIAL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462511"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VLA60_05400"
     CDS             complement(<1..1435)
                     /locus_tag="VLA60_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF08827.1"
                     /translation="MIRILLLLVLDHVRHRPFRAFLTVVGVAIGVSAWLAIRLANGAV
                     YQSFEDSVASVVGEASITLSRGSEGMDESIIEEIQRFPGVQSAQPVLKIESMIKEGPS
                     TGHSLVIWGVDLLNYEELRPSGDSTNAITDNQWKQVFSPQTVFLGEEFAGELGLREGQ
                     TLTVTSQGLSHDLIVGGLLRSSDALFQGTERQAIMDIAATQWLFGWLGRLHSIAVVPE
                     SGVAGATLIQALQKVVPSDIRINQSSRRTEQVESMLKAFQFNLTMLSGIALLVGVFLV
                     YNTMAFSVAHHRREIGILRALGMERRAITGLFLLESGLLGLVGGVLGCWFGVFLAGGL
                     TTLIGQSVGELYGVTSLAVSQMHPWLLVEAIGIGVGVSLLGAFRPSWDASTIAPVQAL
                     SVGQSEDEESVSYRNSGWIAMIAFSGSAALSTMAPVDGIPVGGYAAAFCLLVGGTALG
                     PVLCGVVHEWKRTWQSGRWGLLPSLAAE"
     gene            1508..1801
                     /locus_tag="VLA60_05405"
     CDS             1508..1801
                     /locus_tag="VLA60_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSF08828.1"
                     /translation="MPKGKLTESESTEELLESNSEGQSLTARQKEILSLVAQGFTNRE
                     IGEKLDISVRTVEVHRFNLMRRLRVRNVAQLLRQAIALRIIPQAPTTPPTTKT"
     gene            complement(1906..2835)
                     /locus_tag="VLA60_05410"
     CDS             complement(1906..2835)
                     /locus_tag="VLA60_05410"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HSF08829.1"
                     /translation="MDLSTVIAGVSFPFGVMNASGALCVTPEELVALGMSHAGAIVTK
                     SMTPLAREGNPQPRYVAFDGGSINSMGLPNLGFPVYAKLIPELKRFGKPVIASLAGLH
                     PDDFLEGAKALEIARPDLVEVNLSCPNIPGKPQIGYDFEETERLLTEIRALLTVPFGV
                     KLPPYFDPIHHQKIAEVLQRSGVAFVTVINSVGNTLVVDPDREMVVIKPKGGFGGLGG
                     RIIKPVALANVRAFWKIWGDQIPIIGTGGVEHGVDVFEHVLCGASAVQVGTVLVEEGC
                     EVFARLEREIAACLQGKGYPSLMACRGKLREMD"
     gene            complement(2842..3504)
                     /locus_tag="VLA60_05415"
     CDS             complement(2842..3504)
                     /locus_tag="VLA60_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF08830.1"
                     /translation="MIQFQHVYKEYQRGSTSVVALRDVCVDIQQGEFCALMGPSGSGK
                     STLLHLVAGLDWVTSGDILLDGRSCLGFDDQEWTRWRREEVGIVFQAFHLIPGLTIEE
                     NVALPLRLRGEESETIRTRTADMLERVGMSHRIGHRSHELSGGEQQRVALARAFGHRP
                     RLIVADEPTGNLDGETGEKIVSLLRQCAKEFGQTVLLATHSLRAAQTADRIVAIRDGQ
                     LE"
     gene            3659..4402
                     /locus_tag="VLA60_05420"
     CDS             3659..4402
                     /locus_tag="VLA60_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HSF08831.1"
                     /translation="MAPPFTMTRLLRSLLHVIFPTACAGCQHPLQDDPVPFFCRRCWD
                     RLKPIPGPVCPRCGRPFASPIALLHSPTHQCGACRARPMALTQIWSLFPYQPPLKEAI
                     TLFKYRGKLSLTQPLAQAMVEALPALPTLDAIIPVPLHPQRLREREYNQSLLLANRLS
                     HHLGIPLFLACLLRIRPTVPQTSLSRKERLTNLRRAFSVPKPAPIKGKRILLVDDVLT
                     TGTTLHECAKTLRRAGSGPVYGLTLARMV"
     gene            4703..5050
                     /locus_tag="VLA60_05425"
     CDS             4703..5050
                     /locus_tag="VLA60_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSF08832.1"
                     /translation="MPIYEYRCQDCRKRNSFLILTLNAPVPSCTHCRSTNLERIMSRF
                     AAPKSEEARMEALADPSNFGDLDENDPQSMARFMKKMGKEMGEDLEDDMDEAMEGMDT
                     DDMDMGMPATEGD"
     gene            5146..5586
                     /locus_tag="VLA60_05430"
     CDS             5146..5586
                     /locus_tag="VLA60_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08833.1"
                     /translation="MFAGQYYCKIDEKGRFVVPSPIREEIEIHGNSLMFLKGQDMPVS
                     AYTNPEWAKILENAKQSLDEDQRRLFMYHMVSEASASEMDRAGRILIPGRFRKLVPVD
                     EDQEVVLVGMFHRLEVWNPSEWRRFVHVNEDTFEHSMSKIQSLL"
     gene            5644..6270
                     /locus_tag="VLA60_05435"
     CDS             5644..6270
                     /locus_tag="VLA60_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HSF08834.1"
                     /translation="MRTIRIPSLPKLLQSKGKGGLVLLHSHKRKARTADSGMDWSNLG
                     GDTLSTRELFVVLPLPPSINHQYATVNGRRVLASPGRHYKTAVARHLLTILRQSSHRT
                     EFLEQLETHTLSLSLRFHFKTALRRDLDGGLKIAQDAICQAIDLNDNRITEIHLHKDL
                     DSVRPRLECLLAIQEPIVRTIRSPKNLSRVTKVNPRNVPRSSTDHKPR"
     gene            complement(6493..6903)
                     /locus_tag="VLA60_05440"
     CDS             complement(6493..6903)
                     /locus_tag="VLA60_05440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSF08835.1"
                     /translation="MGTSTAPRQDLRQPIPIPQGDRRSFRRMSVKPYQVLPVAIRYGQ
                     DTVCRGRVLNLSPQGMLVEFPDNQIPSVLRGTQVSVKLHYLGDSIWLPGLVRHCKGQK
                     MGFLFPALTSHPTRAAKHPLTIVLHSLSRAVTSL"
     gene            complement(6906..7271)
                     /locus_tag="VLA60_05445"
     CDS             complement(6906..7271)
                     /locus_tag="VLA60_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF08836.1"
                     /translation="MVISERSLSHDTLVETFMGRFDQHARQDFKWRIDHAILQGYRFV
                     MLNMVAVSFIDSAALGWLVLAQRRFQRIGGKLAIISPIGFVRDVLEITEIGEWIPIFS
                     SEDEALKIFESSKEHPAES"
     gene            complement(7393..8025)
                     /locus_tag="VLA60_05450"
     CDS             complement(7393..8025)
                     /locus_tag="VLA60_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08837.1"
                     /translation="MVSKVELCLHAVQRWGFSFLIAMDLIGICPVSAEVLRATDFESG
                     TLSDWTVFSTSNGTLGGKGFPTVAQCDLGNRGSVSHCLQVQVGQLHFAPAHEVQQGGG
                     INLTTMTNAGLIQLSARVGATYHSPDYKRNLNGGLFEWVVDDQVIADLDVGPIEDGGT
                     VQHHFIGKVPVAAGRHTIQLRVTRPFQSGAGKPSPVQFIDDVLVELLSQP"
     gene            8276..>8798
                     /locus_tag="VLA60_05455"
     CDS             8276..>8798
                     /locus_tag="VLA60_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08838.1"
                     /translation="MSTLFTPLFVFWQIVGGISCLVGLSSPSWAQQTPPEAHPVHPLI
                     SAQVLVVETIALTERGLQDSTAITKTVTDRLADTGFTIVSTPKEPHDAVIRVKCEERQ
                     TFTGPSQHRNTSPALTSRLWKGPACHISYRYGEQFPPWRWDVHTAFEDTREAAEIAGA
                     SDTGTFAMIALNTQ"
BASE COUNT         2231 a         2581 c         2020 g         1966 t
ORIGIN      
        1 gttccgccgc gagggacggc aggaggcccc atcgcccgct ttgccatgtt ctcttccact
       61 catgcaccac tccacagagc acaggcccca gggccgttcc tcccaccaga agacaaaacg
      121 ccgcggcata cccgccgacc ggaataccgt ccacaggagc catagtactc aaggcggcac
      181 ttccactaaa ggcgatcatc gcgatccacc ctgaattcct gtacgaaaca gattcttcat
      241 cttcggattg gcctaccgat aaggcttgaa ccggggcaat cgtgctggca tcccaggaag
      301 gacggaacgc tcccagaagg gaaaccccga ccccaatccc tatcgcttcc accaacagcc
      361 agggatgcat ctgggacacc gccaaagacg tgaccccgta taattcgccg acactttgcc
      421 cgatgagcgt ggttaacccg ccggccagga agaccccgaa ccagcagccc aagactccac
      481 ccacaaggcc gagcagaccc gactccaaga gaaacaatcc agtgatggct cgccgttcca
      541 tgccgagtgc ccgaagaatg ccaatctccc gacgatggtg tgccaccgaa aatgccatcg
      601 tgttatagac cagaaacact cccactaaca gcgcaattcc actgagcatg gtgagattaa
      661 actggaaggc tttcaacatc gattccactt gctccgtccg tcgcgaggat tgattgattc
      721 gaatatccga cggcacgacc ttttgtaagg cttgaatgag cgtggccccc gccacaccag
      781 attcagggac tacggcaatg gaatgcagac gccccaacca accgaacaac cattgggtgg
      841 cggcaatatc catgatcgcc tgtcgttcgg tgccttggaa aagggcgtcg gaggacctca
      901 acagaccccc cacgatcaga tcatgggaaa gaccctgact cgtcacagtc agtgtctgcc
      961 cttcgcgcaa ccctaattcc ccggcaaact cttcccccaa aaagaccgtc tgcggcgaaa
     1021 acacctgttt ccactgattg tccgtgatcg catttgtcga atcccctgat ggcctaagct
     1081 cctcatagtt cagaagatcg actccccaaa tcacgagcga gtgacccgtg gaggggcctt
     1141 ctttgatcat ggactcaatt ttcagaactg gctgagctga ttggaccccg ggaaaacgtt
     1201 gaatctcctc gataatcgac tcatccatcc cctccgaccc acgtgatagg gtgatggacg
     1261 cctctccaac aaccgacgcc accgaatcct caaaggattg atacactgcg ccattggcca
     1321 ggcgaatggc caaccaggcc gaaaccccga tcgccacccc gaccactgtg agaaaggctc
     1381 ggaagggccg atggcgcaca tggtcaagga ccaatagaag aagaattcgt atcatagaag
     1441 cggttgtcgg aagtaatgga cgtatgttaa aacaactaga aaattatgcg tggggcacgg
     1501 actcaatatg ccaaaaggga aattgactga atcggaatca acagaagaac tcttggaatc
     1561 aaattccgag ggacaatctc tcaccgctcg gcaaaaggaa atcttgagcc tggtggctca
     1621 aggctttacc aatcgtgaaa ttggtgaaaa gctcgacatt agcgtgcgaa cggttgaagt
     1681 ccaccgcttc aatttgatgc gccgcttacg tgtcagaaac gtggcccaat tactccggca
     1741 agccattgcg cttcgaatca tcccccaagc cccgacgacg cctccgacga caaagacctg
     1801 agatacgtgc atttcgaccc agcctgctca tcattgaggg ttgaaaaacg cagctgaacg
     1861 catatattga ctctaccctg tgaaggcgtt cgatcgtatt tcgcttcaat ccatctcccg
     1921 taatttccct cgacacgcca tcaatgacgg ataacctttg ccttgcaggc aagcggcaat
     1981 ttcccgttca agccttgcaa acacctcaca accctcctca accagcacgg tgcctacctg
     2041 aactgctgag gcgccacaca acacatgctc gaacacatcc acgccgtgtt ccaccccacc
     2101 tgtgccaata ataggaatct gatcgcccca gatcttccaa aacgcgcgaa cattggccag
     2161 ggccacaggc ttgattatcc taccacccaa tccgccaaag ccgcctttgg gcttaatcac
     2221 caccatttcc cggtcgggat cgaccaccag ggtattcccc actgaattga taaccgtaac
     2281 aaaggccact ccacttcgct gaagcacctc ggcaattttc tgatgatgga tcggatcaaa
     2341 gtagggcggg agtttcactc cgaacggcac cgtcagcaac gcacgaattt ctgttaataa
     2401 ccgttccgtt tcctcaaagt catagccgat ctgtggctta ccgggaatat tgggacacga
     2461 caaattcact tccaccaaat ccggtcgagc gatctccaag gccttggcgc cttctagaaa
     2521 atcatcggga tgaaggccgg ccagactggc gatgacgggt ttcccaaacc gcttcaactc
     2581 tgggatgagt ttcgcgtaga cgggaaatcc taaattggga aggcccatcg aattgatcga
     2641 gccgccgtcg aaggccacat acctcggttg aggattcccc tcacgggcta gcggcgtcat
     2701 tgatttggtc acaatcgcac cggcatggga catgcccagg gccaccagct cttccggtgt
     2761 cacgcacaag gctcctgacg cgttcatcac tccaaatgga aacgacacac cggcaattac
     2821 tgttgaaaga tccatttcgt attactccaa ttgtccgtct cgaatcgcaa caatccgatc
     2881 cgctgtctga gcagcgcgta atgaatgggt ggccaagagc accgtctggc cgaattcctt
     2941 cgcacattga cgcagcaacg acacaatttt ctctccggtt tcaccatcca aattaccggt
     3001 gggttcatca gccacaatca gtcgagggcg atgcccaaag gcccgggcaa gcgctacccg
     3061 ctgctgttct cctccggata attcatgcga ccggtgaccg atccgatgac tcattcctac
     3121 ccgctccagc atgtcagcgg tccgggttcg gatcgtttct gattcctccc ctcgtaaacg
     3181 caagggaagt gcaacatttt cttcgatcgt caatcccggg atcagatgga aagcctgaaa
     3241 cacgatcccc acctcctccc gacgccatcg ggtccattcc tgatcatcaa accccagaca
     3301 ggaacgccca tccagcagga tatctccgga cgtcacccag tccagcccag ccaccaaatg
     3361 caatagcgtg cttttaccac tcccgctggg tcccatgagc gcacaaaatt ctccctgctg
     3421 aatgtccaca cagacatccc gcagcgccac gaccgaagtg gatccgcgct gatattcttt
     3481 atagacatgt tgaaactgaa tcacgtgaac ctatcagaat tttctgccct cagtaacgct
     3541 cgtgaatcat ggcatattcg ccatctctcc tatatcataa cgaaggtaaa tggaccaagg
     3601 cgccccacga taattaggca tactccacgg ttgacctcag ctcggaaagt tcgcctccat
     3661 ggctccacca tttaccatga cacgtctact ccgaagtctc ctgcatgtca tttttcccac
     3721 cgcctgtgcc ggctgtcagc atcccttgca ggacgatccc gttccgtttt tttgccggcg
     3781 gtgttgggat aggctcaaac ccattccagg cccggtttgt ccgagatgtg gacgcccctt
     3841 tgcctccccc attgccctat tacatagtcc cactcatcag tgcggagcct gccgcgcacg
     3901 accaatggct cttacgcaaa tctggagcct ctttccctac caacccccac tgaaagaagc
     3961 cattacccta ttcaaatacc ggggaaaact gtcgttgact caaccactcg cccaagccat
     4021 ggttgaagcg ttgcccgctt taccgacctt agatgccatc attcccgtcc cccttcatcc
     4081 gcaacgctta cgagaacgtg aatacaatca atccctgctc ctcgcaaacc gactgagtca
     4141 ccacctgggg attcctcttt tcctggcgtg tttactccgg attcgtccca cagtccccca
     4201 aacctcgtta tcaaggaaag agcgactcac gaatctgcgt cgggcttttt ccgtccccaa
     4261 gccggcccct atcaaaggca aacggatctt attggtagac gatgtcttaa ccactggcac
     4321 aaccctgcat gaatgcgcca agacgcttcg acgtgccggc tcaggcccgg tgtatgggct
     4381 gacacttgcc agaatggtgt aagaaatttg ttgcaagtcc ctactggagg tcgaggcaaa
     4441 tcgtctgcca acatggatcg actgctggct gttttctctg aataaattcc aactcacgtc
     4501 cttctcccct tgtctgtctt ttctcgattg gccttttcac cagccccttc tatgatggtc
     4561 ttagaagaaa aagtccgata cctccgctct attaacgtat ttgagatcct ttcgaatcgt
     4621 ataaaccaat tttcgactct tcccaaaggg accattttgc gatatataca gcccgtacat
     4681 tgtgatcagg aggaacgact caatgcccat ttacgaatac cgttgtcagg attgccggaa
     4741 gcgcaactcc tttttaatcc tcaccttgaa cgccccagtc ccatcctgca cgcattgccg
     4801 aagtaccaac ctggaacgca tcatgtcccg cttcgctgcc cccaaatcgg aagaagcccg
     4861 gatggaagcc ttggccgatc ccagtaactt cggggacctg gacgaaaacg atccacagag
     4921 catggcacgt ttcatgaaaa aaatgggaaa ggagatgggg gaagacctgg aagatgacat
     4981 ggacgaagcc atggagggaa tggacactga cgatatggac atggggatgc ctgccaccga
     5041 aggcgattag ctcgactact caagcaaaac tacttgactt ttcttaaact ttcggtaagc
     5101 tacattcaca gataggacgt tcaggagatc caaggaacat tctgcatgtt tgccggacaa
     5161 tattattgta aaatcgacga aaaaggacgg tttgttgtcc cgagtcctat ccgtgaagaa
     5221 atcgaaattc acgggaatag tctgatgttt ctcaagggac aggatatgcc cgtctcagcc
     5281 tataccaatc cggaatgggc caagatcctg gaaaacgcca aacaatctct tgatgaagac
     5341 caacgacgtc ttttcatgta tcacatggtc tcggaagcct ccgcatcaga aatggatagg
     5401 gcaggtcgca tcctcatccc gggccggttt cgcaaactcg tcccggtgga tgaggatcaa
     5461 gaagtggtat tagtgggcat gtttcatcgc cttgaagtat ggaatccatc ggaatggcga
     5521 cgttttgtcc atgtaaacga agacaccttt gaacacagca tgagcaaaat tcagagcctc
     5581 ctctaacaga cccgttgctt ctcgttccac caccgatacc ataccccttt catagatgcc
     5641 ccattgagaa ctattcgtat tccctccctt cccaagcttt tgcagtccaa agggaagggt
     5701 ggcctagtcc tcctccacag tcataaacga aaagccagaa ctgcagattc cgggatggat
     5761 tggtcgaatc tgggcggcga tactctgtcg actcgcgaac tttttgttgt cttaccgctt
     5821 ccacccagca ttaatcatca atacgccacc gtcaacggcc ggagggtcct ggcctcccca
     5881 ggccggcatt ataagaccgc cgtggcccga cacttactca ccatcctgcg gcaatcatct
     5941 catcggacgg aatttttaga gcaattggag acccacactc ttagtctctc cctccgcttc
     6001 cacttcaaaa cagccctgcg acgggatctg gatggcgggc tcaaaatcgc gcaggatgcc
     6061 atttgccaag ccatcgattt aaatgacaac cgcatcaccg aaattcatct acacaaagac
     6121 ctggattcgg tccgtccccg cctagaatgc ttattagcca ttcaggaacc catcgtccga
     6181 acaatccgat cacccaaaaa tctctcgagg gtcaccaaag taaatccccg aaatgttcct
     6241 cggtcctcca cagaccataa gcctcgttga aatccagatc cgcccgtccc tctctaacgg
     6301 cggatattcc atgtgccatt ggtccttcca attcccattc gccagacttt gctttgcaat
     6361 tagctgaaga cggaacccgt cataggtggt ggctttgacg aggacgcttc aggctgatgc
     6421 cgccctgttc agtagtcaga tgaatatctg ctcacgatcg gggacagggc tgaaatgatg
     6481 gaaaagaaga aattagagag aggtgactgc gcgagagaga gaatgcagaa cgatggtcaa
     6541 tgggtgcttg gccgctctcg tgggatggct ggtcagggcc gggaacaaaa atcccatctt
     6601 ttggcccttg cagtgccgga caagtccagg caaccaaatg ctatcgccga ggtaatgcag
     6661 tttgacagac acctgggtcc ctcttagcac agaagggatt tgattgtcgg ggaattcaac
     6721 gagcatgccc tgagggctga gattgagcac acggccccga cacactgtgt cttgcccata
     6781 acgaatggca accggaagca cttggtaggg cttgacagac atccggcgaa acgatcgtcg
     6841 gtctccttga ggaatcggta tcggttgccg gagatcttgt ctcggagcgg tcgatgttcc
     6901 catcgtcacg actccgctgg atgttctttg ctggattcaa agatcttcaa ggcttcatcc
     6961 tcactagaaa agatggggat ccattcccca atttcagtga tttccaagac atcccgaaca
     7021 aaccctatcg gagaaataat ggccagcttg ccaccaattc gctgaaagcg ccgttgggcg
     7081 agaaccaacc atccgagggc agcgctgtca ataaatgata cggccaccat atttaacatc
     7141 acaaatcgat aaccttgcag aatggcatga tcaatccgcc atttaaaatc ctgtctggca
     7201 tgttgatcaa atcgacccat aaaagtttcc accagggtat cgtggctgag actccgttcg
     7261 gaaataacca tagaagattc tttcggggtg atattgggtg gtgtcttcct gtatcggttt
     7321 tcctcgctcc agtcttaacc cggcataccg gcatagaaca tgctttatct tcaataagct
     7381 tgaaggggcc tttcagggtt gggaaaggag ttccaccagg acatcatcta taaactgaac
     7441 tggtgacggc ttgccggcac cgctctgaaa gggacgggtc acccgaagct ggatggtatg
     7501 acgacctgct gccacaggga cttttccaat gaaatggtgc tgaaccgtgc caccatcctc
     7561 tatcggaccg acatcaagat cggctatgac ttgatcatca acaacccatt caaacaatcc
     7621 cccgttcaga ttccttttat aatcgggtga atggtaggtg gcccccacac gggccgaaag
     7681 ctggatgagg ccggcatttg tcattgtggt gagattaatc cctccacctt gttgcacttc
     7741 atgggcagga gcaaaatgaa gctgaccgac ttggacctga aggcaatgag agacagaacc
     7801 tcggttccca agatcacact gtgcaacggt aggaaatccc ttgcccccta aggttccatt
     7861 ggaggtagaa aacaccgtcc agtcagaaag ggtccccgat tcaaaatccg tggccctgag
     7921 gacctcggcc gatacagggc aaatcccaat caagtccatg gcaatgagaa aagagaagcc
     7981 ccatcgttga acagcatgta ggcacaattc gacttttgaa accatagcgt tatttccctc
     8041 cataacctgc acctttttgc ttctcatagt ttttttcaga cttgaatgcc tgataagata
     8101 tgtaataacg ggtggctagt tccaggtttc tccgtttgag tctctactca gaccacagat
     8161 tctcccctta agaaccccct tttttccgtt ctcaccgacg gtttcaggga cagagtagat
     8221 tcgtgcacga cactattcat tttttcattc acccgaacat caaggtccgt acaatatgag
     8281 cacactcttc acacctctgt tcgtcttttg gcaaatagtc ggaggaattt cctgtctggt
     8341 gggtctttcc tcgccgtcct gggctcaaca aacaccaccg gaggctcatc cggttcatcc
     8401 cctgatatca gcccaggtct tagtggtcga aaccatcgcg ctcacggagc gaggactcca
     8461 ggactccacg gctatcacaa agaccgtcac cgaccgattg gccgacacag gatttaccat
     8521 cgtctctact ccaaaagaac ctcacgatgc tgtcatacgg gtgaaatgcg aagaacgaca
     8581 gacattcacc ggcccgagtc aacatcggaa taccagtccg gcccttactt cgcgattatg
     8641 gaaaggccct gcctgtcata tttcttatcg ctatggagaa caatttcctc cttggcgctg
     8701 ggatgttcac actgcatttg aagatacacg tgaggctgcg gaaatcgcag gagcctcgga
     8761 tacgggaacc ttcgcaatga ttgctttaaa cacccaac
//
LOCUS       DATIAL010000176         9194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1107580, whole genome shotgun sequence.
ACCESSION   DATIAL010000176 DATIAL010000000
VERSION     DATIAL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107580"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="VLA60_05460"
     CDS             <1..1732
                     /locus_tag="VLA60_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08839.1"
                     /translation="ALPVYRNHELASTLELLSSENLQDSPKVSSKQSSLIGIIQIGIT
                     TAPMQQSLNETVWNIGLLTLGIIFVGILLTILLANRIVTPLRRLAEMSHKIAEGDLSV
                     TVTADTQDEVGQLTTSINRMARALQQREEAISHYVHTITKQVTQLSTLHQTGIVITST
                     LDIQKLFSTVLKLLRKNLGFQRMILVLQNPGGTKAIVSDVSGVPLAIEQQIRHLEFPI
                     SPGSIDETLLLHGRPVLAYDLEEIAHQMSPLILPICRQMEVISFVCAPLLSHQRVLGY
                     LGADRGHQRCTQEDLDLLLTIASHVAVAIDNAQTYEEVEKLAQTLEERVKERTQDLQS
                     ANERLQELDRLKSSFVSIVSHELRTPMTSIKGLVENMLDGLVGNLNDRQSFYLERMKY
                     NIERLTRMINDLLDLSRIEAGRMDLHRGPVNMGSLAREVVETLQSIAQERSLILEAHI
                     SDPIGFIQGDRDKLIQIFTNLINNALKFTESPGTVTVEVNQRDDGMIQTCVIDSGCGI
                     PLEEQQTIFERFYRGQSSEMKDRGAGLGLAITKSLVELHGGSIWVESTLGEGSRFCVI
                     LPVTPSTTPS"
     gene            1961..2851
                     /locus_tag="VLA60_05465"
     CDS             1961..2851
                     /locus_tag="VLA60_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08840.1"
                     /translation="MSTLSMKPRILLVDDDTDILLALSDYLRQEGFEVDSVETGGAAL
                     QKSTTASYDIVLLDVGLPDRDGIEVLNELSQTHPHLPVILLTAFTSLRRTAPPDILDK
                     AFAYLTKPYQRQEVKSAIHRACLARNIAMAGWQGSFAPTHPITTFPSLLQPSSTGASL
                     HDATQHPLNLTAYEQLAEYAQLMQFAFDHVPNGIMVADSDKRLRFANQAACDALGYTQ
                     EELKTLRIPDIAPYHDNEKFQQRLESLRKGQPLSYPSIHRNKNGQEFPIEISVYLLNF
                     QGQEFTCAVTRPVVEKSPKA"
     gene            complement(3555..4964)
                     /locus_tag="VLA60_05470"
     CDS             complement(3555..4964)
                     /locus_tag="VLA60_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF08841.1"
                     /translation="MKKKILIVDDHPDIVLMLTDRLEALGYDTISAGNGQEALEKYEQ
                     DFPHLMLLDLEMPQMAGMEVLHQLAKMSVKKNGEADSQTFGGGECLPLPVVVMTAHGT
                     ISKAVEAMKAGAYDFLTKPIELDHLAIVLKKVLTREALGRQVAALRTEVESRYSQIVG
                     NSSQVHSMVELAKRAAEADATVLLLGESGTGKELFARSIHQWSDRRDMPFSIINCVAL
                     TETLLENELFGHEKGAFTGADSLQKGKIEAADGGTVFLDEIGDMPLGLQAKLLRVLQD
                     HEFPRVGGTRLVRVNIRVIAATNKDLKRAVKERTFREDLYFRLNVVNLLLPPLRDRPE
                     DIIPLAEHFLARHMREMKKRRRSFSKSAIKTMRCYAWPGNIRELDNAIARAVVLGVEE
                     EISPDLLGLGGGKDELEEIDNLPYHESLDRFGTRILEQAMRRSNWSQTKASELLGLQR
                     TYLSRLLKQKGIQQGTESS"
     gene            complement(5035..6012)
                     /locus_tag="VLA60_05475"
     CDS             complement(5035..6012)
                     /locus_tag="VLA60_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08842.1"
                     /translation="MTRAKSLPTGTSPKLNLARLRTYPLQTRHSKVKMADFGSPWHRA
                     GSFQAFLKTLPDILAGKTFRAVVAAIVDARRRGKPVILGMGAHPTKVGLNPIIVDLMR
                     KGVITAVAMNGAVIIHDFEIAYLGQTSEEVEAEIDSGRFGMAEDTGRILNEAIVQGWQ
                     QGMGIGEAVGRYLLKYPRQFPHRRRSLLAAGAQLGLPVTVHVAIGTDIIHMHPQANGE
                     AIGGGSLLDFRKLAAVVSKMEGGVYINLGSAVMMPEVFLKTVTLGRNLGRALKQITTV
                     NMDFLPHYRPMTNVVKRPTQKGGKGYALTGHHELMVPLLAAAVLEALGR"
     gene            complement(5999..6580)
                     /locus_tag="VLA60_05480"
     CDS             complement(5999..6580)
                     /locus_tag="VLA60_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HSF08843.1"
                     /translation="MNIDAVIQAFETSADVKNEFIRTHAERVVEVGQLLIRAFREGRK
                     VLLFGNGGSATDASHLAAEFVGRYRRDRDPLPALALACDMAAITCIANDYDYTDIFSR
                     QIKAHGRKGDVAIAISTSGNSLNVIRGVEAATNSGLVTVAWTGATGGKVGDLVDYCFR
                     VPSSVTARIQECHITLGHVLCEFIEERLFGDQG"
     gene            6717..7979
                     /locus_tag="VLA60_05485"
     CDS             6717..7979
                     /locus_tag="VLA60_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HSF08844.1"
                     /translation="MSAHIPTKALLLAIQADPTSAIAIVRHLNPDMVCFFLHESHKQV
                     VESQIPPALSRMPQRWDWIFTPSPESFSACQKALAHGLGPLLTTWNVMPGELVIDITS
                     ATVGMASAMVLTGFPFSTKVLSFPGHPFPSTDQAVEAITKLLSQSEASANPWDEEAPH
                     LRHETCYHFNHGSYDAAVKGFHTLEHRISGGLKPFYRALADVAQGYGLWDQLLYRPAW
                     EKLKGGIKALELASVWGGPPGMDRLIHLLKVNLTFLERIVLDSKDIKPAVAFDLLAWA
                     KRRGDRGRDLEVATHVLLRALEAFAQSRLYTQYHLKSWDVNLEQLPEDLRETCCRQYL
                     NEIDGKYRLPLQAQFQALAALGDPIGQRFVTDWSKMKSLFDAADHALLGYGFEPIKPE
                     RFHQLYDLVIKLSGVGLTDLPEFPTMNV"
     gene            7998..8402
                     /locus_tag="VLA60_05490"
     CDS             7998..8402
                     /locus_tag="VLA60_05490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HSF08845.1"
                     /translation="MEVRVYYEDTDCGGVVYYANYLKYFERARTHYFDSHGLSVAALI
                     QEGTEFRVIRAELSYRSAATYGDILEVETTVAADRRTSLTFSHVIKDRTSKRLVVEGS
                     ATLVAVDTNGKIKRLPPPILKVLGLPSGFPFS"
     gene            8415..9107
                     /gene="lipB"
                     /locus_tag="VLA60_05495"
     CDS             8415..9107
                     /gene="lipB"
                     /locus_tag="VLA60_05495"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HSF08846.1"
                     /translation="MALVDSRQPGMVLTFPQLLYAEALELQQSLVAQRLEDRRPDTLV
                     LTEHEPVITLGRTTQPEHWEPRWTELQHMGVHLHQTGRGGSVTYHGPGQIIGYPILRL
                     RNFCPGPKIYVQQLEEVLIRTLAEWGIAGCRHESFRGVWVKTSAGRMEKIASIGVRIS
                     RGVTMHGFALNVNVDLQPFTLLAPCGIEHCVMTSMEKLLGKPIDGTQVREQVAAHFAD
                     IFRLNWTEQQWG"
BASE COUNT         2258 a         2580 c         2139 g         2217 t
ORIGIN      
        1 cgcccttccc gtttaccgaa atcatgaact agcgtcgact ttggaactgc tgtcctcaga
       61 aaaccttcaa gacagtccca aggtttcctc gaaacaatcc agcctcatcg gcatcattca
      121 aatcggcata accactgcac ccatgcaaca atccctcaac gaaacagtat ggaatatcgg
      181 tctgctcacc ctgggaatca tctttgtagg tattcttctc accattctgt tagccaatcg
      241 catcgtcacg cctcttcggc gtttggcgga aatgtctcat aaaatcgccg aaggagattt
      301 gtccgtgacg gtcactgcag acacgcagga tgaagtggga caactcacca ccagcatcaa
      361 tcggatggcg cgagccctcc aacaacgtga agaagccatc tctcattatg ttcacaccat
      421 cacgaagcag gtcacacaac tcagtacctt gcatcaaacc ggaattgtga ttacctcgac
      481 tctcgatatc caaaaactgt tttccaccgt cctgaagctt cttcgaaaaa atctgggatt
      541 ccaacgcatg atcctggttc tccagaaccc tggaggcacc aaggccatag tttccgatgt
      601 gtcaggggta cctctggcga tagagcagca aattcgacac cttgaatttc ccatttctcc
      661 aggaagcata gatgaaaccc ttttgctcca cggacgtccg gtcttggcgt acgatctgga
      721 ggaaatagcc catcagatga gccctctcat tcttcccatc tgtcgacaaa tggaagtgat
      781 ctcttttgtg tgcgccccgc tgctgagtca tcaacgcgtg ctgggatatc tgggagctga
      841 tcggggccat caacgctgca cacaggaaga tctggatttg ctgttgacca tagccagtca
      901 cgtggcggta gccatcgata acgcacaaac ctatgaggaa gtcgaaaaac ttgctcaaac
      961 gctcgaagaa cgagtcaaag aacgcacgca agacctgcag tccgccaatg aacgtcttca
     1021 ggaattagac cggttgaagt cctccttcgt gtctattgtc tcacatgaac ttcgcacgcc
     1081 gatgacctct attaaagggt tggtggaaaa catgttggat ggcttggtcg gtaatctgaa
     1141 tgaccgacaa tccttctatc ttgaacggat gaaatacaat atcgaacgac tcactcggat
     1201 gattaatgat ctcttggacc tttcccgcat cgaagcaggc cgcatggact tgcaccgggg
     1261 ccccgtcaac atgggcagcc tcgccagaga ggttgtggaa acgttgcaat ctatcgcgca
     1321 ggaacgttcg ctcattctcg aagcccatat ttccgatccc atcggattca tccagggcga
     1381 tcgggataaa ctcattcaga tttttaccaa cctcatcaac aacgccctca aatttacaga
     1441 atctcccgga accgtgaccg tggaagtcaa ccagcgggac gacggcatga ttcaaacatg
     1501 cgtcattgat agcggctgcg gcattcctct cgaggaacaa cagacgattt ttgaacggtt
     1561 ttaccgtggg caatcctctg aaatgaaaga tcgcggagct ggcctcggcc tggctattac
     1621 gaagagttta gtcgagctgc acggtggatc gatttgggtg gaaagtaccc tcggagaggg
     1681 tagtcgcttc tgtgtgatct tacccgtaac accctcgact acaccctcct aagaatcacc
     1741 aaggcggtct gtcaaacatg tatccgtttc gatacaacag gtatctgaac agatacatta
     1801 ttaattttga caatttcctc tttcctcgaa aaacaaacat ttacgcaagc ctataaattg
     1861 cagagatttt tgaatggctg gaggaaagta ggcatatttc caggtgactt tttcttccaa
     1921 aattttacaa tcctctaatt cgctcccaaa gaaaggaaac atgtcaacct tgtccatgaa
     1981 gccgcgcatt ttgctcgtgg atgacgacac cgacattctt cttgccctct ctgactacct
     2041 tcgccaggaa ggctttgagg tggattctgt agagaccgga ggggccgccc tccaaaaaag
     2101 tacgacagca tcgtatgata tcgtgcttct tgatgtcgga cttccagatc gggacggcat
     2161 tgaagtgctc aacgaactct ctcagacgca tccacacctg cccgtcatcc tgttgaccgc
     2221 ctttacctcc ttacgaagga ctgcccctcc ggacattctc gataaagcct ttgcttatct
     2281 caccaaaccg taccaacggc aggaagttaa atccgccatt catcgagcct gtttggcacg
     2341 aaacatcgcg atggcaggct ggcaaggctc tttcgcaccc acccatccca ttactacctt
     2401 tccttcgctt cttcaaccaa gctcaaccgg ggcttccctt catgacgcaa cgcagcaccc
     2461 attgaacctc accgcatatg agcagctggc agagtacgct caactgatgc aattcgcttt
     2521 tgatcatgta cccaatggaa tcatggtggc agattcggat aaacgcttac gttttgccaa
     2581 tcaggccgct tgcgacgcct taggctatac acaggaagaa ctcaaaaccc ttcgaattcc
     2641 cgacattgcc ccctaccatg acaatgaaaa attccaacaa cggctggaaa gcttgcgaaa
     2701 gggtcaaccc ttatcgtatc catccatcca ccgcaacaaa aatggccagg aattccctat
     2761 tgagatttca gtgtacctgc tcaattttca aggccaggaa tttacctgcg cggtaacccg
     2821 tcctgtagtg gagaaatcac cgaaggccta aaattttgtt cggcacccag gtcctcgtac
     2881 ctctctgtac aaggccgggt ctgaaaccat ataacccgct caaaaagctc acacgactga
     2941 gaatctgtcc gcagagaaat gatatcaaag gaattgaata cgactactca ggaaggaacc
     3001 aaaaagcatg agggactttt gagtgtagct gaggaaattc atggggttaa gcaagctgca
     3061 accctcaatg atgaagctgg aattcagaat gcctttcaaa agccttcacg aatatcagga
     3121 tatggctgtg tctgtttttt gccttcctct gctcggcggc ggatctgaaa ccccatttct
     3181 accctgaccg aagtaaccga ccacttggca ttctttcaaa agaagaaaag tctccagcct
     3241 tttgatattg gagaacttct tgcccctcac ccaacccgga tccaccacca cggtttctac
     3301 aggctcggca taaggtattt cagttgccaa gggcttatga tgtttcccat gtttatcctg
     3361 actgattccc actatcccca caaattggtt ttccggaacc cagcaggcgg tttttcattg
     3421 ggatatcagt acgaatgcca gcattcggac caattccaaa taggacaata ggtcctcatt
     3481 cccctttagg agtaaataat tggtcgtttg aaccttggag agtctttccc cggttcctcg
     3541 aaatccaaaa atgattagga actctcagtc ccttgctgaa tgcctttttg tttgaggagg
     3601 cgagagaggt aagtgcgttg aagtccaagg agttctgagg ctttggtttg gctccagttg
     3661 cttcgacgca tggcctgctc cagaatgcgg gtaccgaacc ggtccaggga ttcatggtag
     3721 gggagattgt cgatttcctc gagttcgtct tttcctcccc ccaatcccaa gaggtctgga
     3781 gaaatttctt cctctactcc caacaccact gctcgagcaa tcgcattgtc cagttcccga
     3841 atattgccgg gccatgcgta gcatcgcatc gttttaatgg cagacttgga aaagctccgc
     3901 ctccgctttt tcatttcccg catgtggcgg gctaaaaaat gttcggcaag aggaatgata
     3961 tcttcgggac gatcccgcaa tggaggcaac aacaagttca cgacattaag ccggaaatac
     4021 aggtcttccc gaaaggtcct ctctttaacc gctcgtttca gatccttgtt ggtggccgca
     4081 atcacccgaa tgttgacgcg gaccaatcgc gtcccaccta cccggggaaa ctcatgatcc
     4141 tgaagcaccc ggagtaattt agcctgaagc cccagcggca tatcgccaat ttcatccagg
     4201 aaaacggttc ccccatccgc ggcttcgatt ttccctttct ggagagagtc tgcaccggta
     4261 aacgccccct tttcatgccc aaagagttcg ttctccaaga gggtttccgt gagagccacg
     4321 caattgatga tgctgaaggg catatcccgc cggtcgctcc attggtgaat ggatcgggca
     4381 aacaattctt tccccgtccc gctttctccc agcagcaaca ccgtggcatc agcttcggca
     4441 gcccgttttg ccaactccac cattgaatgc acctgagagc tattccccac gatttggcta
     4501 taccggcttt ccacctccgt gcgcaacgcg gcaacctgcc gccctaaggc ttcccgcgtc
     4561 aggacctttt tgagaacaat ggccagatgg tccagctcaa taggtttcgt gagaaagtca
     4621 tacgctccgg ctttcatggc ttcaacggct ttggaaattg tgccatgagc cgtcatgacc
     4681 accacaggca acgggaggca ctctcctccg ccaaaggtct gagaatcagc ctctccattt
     4741 tttttcacac tcattttggc taattggtgc aacacttcca tgccggccat ctgtggcatt
     4801 tccaaatcca agagcatgag gtgaggaaag tcctgctcat atttctccaa cgcttcttgg
     4861 ccatttccag cggaaatcgt gtcataaccc agggcctcta agcggtctgt aagcatgagg
     4921 acaatgtcgg gatgatcatc gacgatgaga attttctttt tcatgagggt tctcctcctt
     4981 aagcccgcac ctgattatcc catgtctata aattcgcagc cacctgagaa tacctcacct
     5041 gcccaacgcc tccagcacgg ctgcggccaa aagcggcacc atcaattcat gatggccggt
     5101 cagcgcataa ccttttccac ctttttgcgt tggccgtttg acaacattgg tcatcggccg
     5161 gtaatggggg agaaaatcca tattcaccgt cgtgatctgt tttaaggctc gtcccagatt
     5221 ccgccccaac gtcacggttt tgagaaagac ttccggcatc atgaccgctg atcccaaatt
     5281 gatatacacg cccccttcca tcttcgacac cactgcggcc agcttgcgaa aatccaataa
     5341 cgagccgcca ccaatcgctt cgccgtttgc ctggggatgc atgtgaataa tatcggtccc
     5401 gatcgccaca tgcaccgtga cgggaagacc aagttgcgca ccggccgcca gcaagctcct
     5461 tcgccgatgt ggaaattgcc tgggatactt caaaagatac cggccgaccg cttctccaat
     5521 gcccataccc tgttgccatc cctgcacaat cgcctcattt aaaatgcgtc ccgtgtcttc
     5581 tgccatgcca aatcgtccgg aatcaatttc ggcttcgacc tcctccgagg tttgccccag
     5641 gtaggctatc tcaaagtcgt gaataatgac ggctccattc atggccaccg ccgtaatcac
     5701 acccttgcgc atcaggtcca cgataatggg attaagaccg accttggtcg gatgggcgcc
     5761 catgccgagg atcaccggtt ttccccgtcg tctggcatca acgatcgccg ccaccacggc
     5821 tctgaaagtt ttgcccgcta aaatatccgg taaggtttta aggaaggctt gaaaggaacc
     5881 agcacggtgc cagggtgaac caaaatccgc cattttcacc ttggaatgac gggtttgaag
     5941 cgggtaggtg cgaagccggg cgagattcag cttcggagac gtaccggtcg gcaatgattt
     6001 agccctggtc accaaaaaga cgctcttcaa taaattcgca caacacgtga cccagcgtaa
     6061 tatgacattc ttgaatacgt gccgtcaccg aagaagggac tcgaaaacaa taatcgacaa
     6121 gatctccgac tttacccccg gtggcacctg tccaggccac ggtaacaagg ccactgttcg
     6181 tggccgcttc cacaccacgg atcacgttca gggagttccc gctggtgcta atggcaatag
     6241 ccacatcccc cttgcggcca tgcgccttta tttgccggga gaaaatatct gtatagtcat
     6301 aatcattcgc aatacaggta atggcagcca tatcgcaggc caacgccagg gcaggaaggg
     6361 gatctcgatc gcgtcgatac cggcccacaa actccgcggc aagatgcgag gcatccgtcg
     6421 cactcccacc attcccaaag agcaagacct ttcggccttc acgaaatgcc cggatcagca
     6481 attgaccaac ttcaacgacg cgttccgcgt gggtcctgat aaattcattt ttgacatcgg
     6541 cgctggtttc aaaggcctgg atgaccgcat caatattcat cggggccgat tctatcagga
     6601 taggaatcaa atttcacact gtgcgaagag aaataccggt attccccgtt gagagaataa
     6661 tatccagcat cggttttctt gccgggctcc tcaaccgatg atatagtcga cccattgtga
     6721 gtgcccatat ccccaccaaa gcactcctac tcgccattca agccgacccg acgtccgcca
     6781 tcgccattgt ccggcacttg aatcctgaca tggtttgttt ttttctgcac gagtcccata
     6841 aacaggtcgt ggaatctcaa atccctccgg ccttgtcacg catgccacag cgttgggact
     6901 ggatcttcac cccttcccct gaaagttttt cggcatgcca gaaagccctg gctcatgggc
     6961 ttggccctct gttaacgacc tggaacgtga tgcccgggga actggtgatt gatattacca
     7021 gcgcgaccgt tggcatggct tccgccatgg tgctgacagg atttcccttt tccaccaaag
     7081 tgctgtcgtt tcccggccat ccttttccat ctacagacca ggccgttgaa gcgatcacca
     7141 aattgttgtc acaatccgaa gcctctgcca atccttggga cgaagaggcg ccgcatctgc
     7201 gacacgagac ttgctaccat ttcaatcatg gctcttatga tgcggccgtt aaagggtttc
     7261 acacattgga acatcgaatc agcggcggac tgaaaccctt ttatcgggcc ctggcggatg
     7321 tggcgcaggg ctatggcctg tgggatcaac tcctgtatcg tcctgcctgg gaaaaattaa
     7381 aaggcgggat caaagccttg gaactggcct ccgtgtgggg cggccctcca ggcatggatc
     7441 gcctcattca cctcctcaaa gttaacctca ccttccttga acgaatcgtg ctggattcaa
     7501 aagatattaa gccggcggtg gctttcgact tattggcatg ggcaaaacgc cgtggagatc
     7561 gcggacggga tcttgaagtt gccactcacg tcctgcttcg cgccttggaa gcctttgctc
     7621 aatcccgttt gtatacccag tatcacctga aaagttggga tgtgaacctc gaacaactcc
     7681 cggaggactt gcgagaaacc tgttgcaggc aatatctgaa tgaaatcgat gggaaatatc
     7741 gtcttcctct ccaggcccaa tttcaggcgt tggctgcgct tggcgatccc atagggcaac
     7801 gctttgtcac ggattggtct aaaatgaaat ccttatttga tgccgctgat catgcgcttc
     7861 tgggttacgg atttgaaccc attaaacccg aacgcttcca ccagctctat gacctggtga
     7921 taaaattaag tggcgtgggt ctcaccgacc ttccggaatt tccaaccatg aatgtgtagg
     7981 ggaggcgacc ggccggaatg gaggtacggg tctattatga agatacagat tgtggaggag
     8041 tcgtctacta cgccaactat ctaaaatact ttgaacgagc ccgaacgcat tacttcgata
     8101 gccatggcct gtcggttgcc gcattaatac aggaaggaac tgaatttcgc gtgatacgtg
     8161 ctgaactttc ctatcgatca gccgccacct atggagacat cctggaggtc gagaccacgg
     8221 tagcggccga tcgacgaacg tctctcacgt tttcccatgt gatcaaagat cgcaccagca
     8281 aaagattggt cgtggaagga tcggcaaccc ttgtggccgt ggataccaac ggcaaaatca
     8341 aacgccttcc acctcctatc ctgaaagtcc tgggccttcc ctcagggttc cctttctcct
     8401 gagaccgcat tcctatggct ctcgtcgatt ctcgacaacc gggaatggtg ctgacgtttc
     8461 ctcagctact ctatgcggaa gccttggagc tgcaacagtc tctggtcgcc caacgtctcg
     8521 aggaccgtcg gcccgacaca ttggtattga ccgaacacga acctgtcatc acattgggac
     8581 gaacaaccca gcctgagcat tgggaacccc gctggaccga actgcagcac atgggggtcc
     8641 accttcatca gaccgggcga ggtggatctg tgacctatca tggccccgga cagataattg
     8701 gatatccgat tttacgactt cgaaatttct gtcctggtcc caaaatctat gtccagcaat
     8761 tggaggaggt cctgattcgc actttagcgg aatgggggat tgccggatgt cgtcatgaat
     8821 cgtttcgagg ggtatgggtg aaaacatccg ccgggcgcat ggaaaaaatt gcctccatcg
     8881 gcgtacgaat ttcccgtggg gtgaccatgc acggctttgc cttaaacgtg aacgtcgatc
     8941 ttcaaccgtt caccctctta gcgccatgtg gcattgaaca ttgcgtgatg acatcaatgg
     9001 agaaactact tggtaaaccc attgatggaa cacaggtgag agaacaagtt gcagcccact
     9061 ttgctgacat tttcagatta aattggactg aacagcaatg gggataaccc gacacagggc
     9121 ttgaattttc catagctaga caacggcact caagcccctt gtccctcgtc cagcgttagg
     9181 aagtccgcct gccc
//
LOCUS       DATIAL010000177         4750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1430889, whole genome shotgun sequence.
ACCESSION   DATIAL010000177 DATIAL010000000
VERSION     DATIAL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430889"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VLA60_05500"
     CDS             complement(<1..449)
                     /locus_tag="VLA60_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HSF08847.1"
                     /translation="MATTDSLVSLCIPARYGSSRFPGKPLALIAGKPLIQHVYEAVQQ
                     VSQVGQILVITDQESIEQGVKSFGGEVCLVKEFCRTGTDRVAKVASQLKYDVIVNLQA
                     DEIPQHPGLLDDLILPFLASRAGVGTLKRQLHRTQDLTNPSIVKVVT"
     gene            complement(436..1449)
                     /gene="rfaE1"
                     /locus_tag="VLA60_05505"
     CDS             complement(436..1449)
                     /gene="rfaE1"
                     /locus_tag="VLA60_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HSF08848.1"
                     /translation="MKPLSVVPSISARKFKEYIQRFSRARILVVGDLILDHYVWGKVH
                     RVSPEAPVPIVHVDSESYRMGGAANVYHNILTLGGQAELCGMVGADHVGKQFLADIRR
                     SSPFTSGVFVDSSRPTIKKTRVVAHNQQIVRFDVEQRHDISSQQTKKIIKHVASRLPE
                     VSCLVISDYAKGMITVDLMKAIHILADQFGVPVVVDPKVEHMAYYHGVTIITPNHLEA
                     KQAAGFLPSQDVPVEQIGIALQQQLQCQAVVVTRGEEGMSIFEKTGQSWTIPAVARQV
                     YDVTGAGDTVISTLALALSAKAPLPEAAVLANQAASIVVGMVGTATVTRSQLQEALLH
                     GHN"
     gene            complement(1503..2264)
                     /gene="lepB"
                     /locus_tag="VLA60_05510"
     CDS             complement(1503..2264)
                     /gene="lepB"
                     /locus_tag="VLA60_05510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HSF08849.1"
                     /translation="MTTQDPQQPKMTESSEKNLQEESSVTSSSQEMEVSKKSIIREYA
                     EALIIAIILALTIRVFVVQAFKIPSGSMIPTLMIGDHILVSKLAYGFQLPKDCEFQVS
                     FPPVTCFSSTIVMNFDPPSRGDIIVFRYPEDENKDFIKRVIGLPGDTIHIQNKHVIVN
                     GQPLLDEDFTQRVDPGIIDGRINPRDNLGPLTVPPDSYFVMGDNRDQSLDSRFWGFVR
                     MDKIKGRAFLVYWSWNGQGAWTDWIRWERIGKIIE"
     gene            complement(2271..4085)
                     /gene="lepA"
                     /locus_tag="VLA60_05515"
     CDS             complement(2271..4085)
                     /gene="lepA"
                     /locus_tag="VLA60_05515"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSF08850.1"
                     /translation="MSKAFPQTHIRNFSIIAHIDHGKSTLADRFLEITGAVSPREARA
                     QYLDAMDLERERGITIKAHAVTARFRSSDGQEYQFNLIDTPGHVDFAYEVSRSLAACE
                     GALLLIDATQGVEAQTIANAYLAISNDLVIIPVINKIDLPSADVEGVKVQIRDVLGLS
                     SEEAFLVSAKDGRGVKEVLEGVVKIIPPPAGSIDQPLKALIFDSWFDNYQGAVVLLRV
                     MDGEITPNMMIKLMFSGREFEVLEVGVFSPKRTKVNRLGPGEVGYICAGMKELSDTKI
                     GDTITDSKRPTSVALPGYRDVKPVVFCGLYTTDNAKFEDLRDSLEKLQLNDSSFVYEP
                     ETSLALGFGFRCGFLGLLHMEIIRERLEREYGLDLISTAPTVVYRVTTTKGETLVIDN
                     PSKLPDPSQIERIEEPYIKATMITPERYIGNVLQLCQERRGIQVGLQYLDPTRSMLIY
                     EIPLNEIVLDFYDRLKSRTQGYASLDYELLGYRESELVKLDLLLNGETVDALSIIAHK
                     DKVQSRGRQLAEKMKELIPKQMFEIVIQATIGKRIIARETIGALKKNVTAKCYGGDIS
                     RKRKLWEKQKEGKKRMKQLGRVEVPQEAFLAILKTDPN"
     gene            4170..>4750
                     /locus_tag="VLA60_05520"
     CDS             4170..>4750
                     /locus_tag="VLA60_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF08851.1"
                     /translation="MTLKTTRTALEKDDCEYLWHPFTQMQEWERQTPLIIKRGKGSYV
                     YDMDGNAYLDATASIWVNIHGHRHPHIDEAIRHQLTQVAHTTLLGLSNPPAIQLAKAL
                     IRLAPKGLQKVFYSDNGSTAVEVAAKMAIQYWQQCPNPQPRKTRFIHLGMSYHGDTVG
                     GMSLSGVELFRRSFSPLLFASHDVEPPYCYRCPL"
BASE COUNT         1242 a         1220 c         1073 g         1215 t
ORIGIN      
        1 ttggttacca cctttacgat tgatggattg gttaagtcct gtgtgcggtg tagttgtctt
       61 tttaacgtcc ccacccctgc ccgagaagcc aaaaaaggaa gaatgagatc atccagcaac
      121 ccagggtgct gaggaatttc atcagcttgt aggttcacaa tcacatcata tttcaactga
      181 gaagccacct tcgccacccg atccgttccg gttcgacaga attccttgac gagacagact
      241 tctccaccaa aactcttgac cccttgttca atggattcct ggtcggtaat aaccaggatt
      301 tgccccacct gcgaaacctg ttgaaccgct tcatagacat gctggataag gggctttcca
      361 gctatgaggg cgagtggctt ccccggaaat cgtgacgatc cgtaacgagc aggaatacac
      421 aggctgacga gggagtcagt tgtggccatg gagtaaagcc tcttgaagtt gcgaccgcgt
      481 cactgtggcg gtacccacca tacccacgac aatacttgcg gcctgattgg ctaataccgc
      541 agcttcagga agtggggctt ttgcactcag agctaatgca agagtgctga tgaccgtatc
      601 gcctgctccg gtgacatcat agacttgtcg agccacggcc gggatggtcc aggattggcc
      661 agtcttttca aaaatactca ttccctcctc tcccctcgtg accacgactg cctggcactg
      721 taattgttgt tggagagcaa tgccgatttg ttcaaccggc acatcctggc ttgggaggaa
      781 tcctgctgcc tgtttagctt cgagatgatt gggtgtgata atcgtcactc catgataata
      841 cgccatatgc tcgaccttcg gatccaccac aaccggcacg ccgaattgat cagccagaat
      901 atgaatggcc ttcatcaggt ccactgtgat catgcccttc gcataatcgg aaatcaccag
      961 gcaggagact tcaggaagac gggaagccac atgttttata attttttttg tctgttgaga
     1021 tgaaatgtca tggcgttgct ccacatcaaa tcgaacaatt tgctgattgt gggctaccac
     1081 tctggttttt ttaatggtag gacgactaga gtcgacaaac acaccggaag taaatgggga
     1141 cgatcgtcga atgtcggcaa ggaattgttt cccaacatga tccgcaccaa ccatgccaca
     1201 taattcagcc tgtccaccca aggtcagaat attatgatac acattcgctg ccccacccat
     1261 tctgtacgat tcagaatcca catgaacaat cggtacaggc gcttccggag agacacgatg
     1321 caccttgccc cagacataat gatcgagaat gagatctccg acaaccaaaa tgcgagcacg
     1381 ggagaagcgt tgaatatact ccttgaattt ccgagcggaa atcgatggga caacggatag
     1441 cggtttcatc cttgagtcgg cacaaggctg aagtttggtt gagtcaagtg agttggtcct
     1501 cactattcaa tgatcttccc gattcgttcc catcggatcc aatcggtcca agctccctgc
     1561 ccattccatg accaatacac gagaaatgcc ctacctttaa ttttatccat tcgcacaaat
     1621 ccccaaaacc gactgtccaa actttggtca cggttatcac ccatcacaaa gtaggagtcc
     1681 ggaggaacgg ttaaggggcc aagattatcc cgaggattga tgcgaccatc aataattcca
     1741 gggtcgaccc tctgcgtaaa atcctcgtcc aacaatggct gtccattcac aatcacgtgt
     1801 ttattttgaa tatgaatggt atcaccaggg agtccgatca cccgcttaat gaaatccttg
     1861 ttttcatcct cggggtaacg aaacacaatg atatccccac gcgacggagg atcaaaattc
     1921 atcacgatcg ttgaagaaaa acatgtcacg ggggggaacg agacctgaaa ctcacaatct
     1981 ttcggcaact gaaacccata ggccagcttg gaaacgagga tatgatcgcc aatcatcaag
     2041 gtcggaatca tcgaccctga tgggatctta aaggcctgga ccacaaagac ccgaatggtt
     2101 agagccagaa taatcgcaat gatcaatgcc tcggcatatt ctcgaataat ggattttttg
     2161 gatacctcca tttcctgtga acttgaggta accgaagatt cctcttgaag atttttttcg
     2221 gaagattcgg tcattttggg ttgttgcgga tcttgagtgg tcatcagcgt tcagttcgga
     2281 tcggtcttta aaatagccaa aaaggcttct tgagggactt caacacgccc taattgtttc
     2341 atccgttttt ttccttcttt ttgcttttcc cacaatttgc gttttcggga aatatctcca
     2401 ccatagcact tggccgtcac atttttcttg agtgcgccaa tggtttccct ggcaataatg
     2461 cgtttgccta ttgtcgcctg aatgacaatt tcaaacattt gtttgggaat gagttctttc
     2521 attttttcgg ctaattgtcg cccgcggctt tgcactttat ccttatgggc gataatggag
     2581 agcgcatcca ccgtctcacc attcagcaat aaatccaatt tcaccaattc agactcgcga
     2641 tagccgagca attcatagtc caaggatgca tacccttgtg tgcgggattt caggcgatca
     2701 taaaagtcta acacaatttc attgagcggg atttcataga ttaacatgga tcgggttgga
     2761 tcgagatatt gcagcccgac ttgaatgcca cgacgctcct gacacaactg aagaacattt
     2821 ccgatatagc gctcaggcgt aatcatcgtg gctttaatat aaggctcttc aattcgttcg
     2881 atctgtgaag gatccggaag tttggaggga ttgtcgatga ccagggtttc gcctttggtt
     2941 gtcgtcacac gataaaccac cgtcggagca gtactgatca aatcgagtcc atattcccgc
     3001 tccaagcgtt cccgaataat ttccatatgg agaagcccta aaaacccaca tcgaaaccca
     3061 aatcccaagg ccagggacgt ttccggttca tagacaaagg aggaatcatt cagctgtaac
     3121 ttctccagag aatcccgcag gtcctcaaac ttggcattgt cggtggtata taacccgcaa
     3181 aagactaccg gtttgacatc tcggtacccg ggcaaggcca cgcttgtggg gcgctttgaa
     3241 tccgtaatgg tatcgccaat tttagtgtcc gatagttctt tcatcccggc acaaatatat
     3301 ccgacttccc cgggccccag ccgattcacc ttggttcgtt tcggtgaaaa gacgccaacc
     3361 tccaggactt caaattcacg tccggaaaac atgagcttaa tcatcatatt tggggtgatt
     3421 tctccatcca tgacccgaag aagcaccacg gcgccctgat aattgtcgaa ccaggaatca
     3481 aaaatcaggg cttttaacgg ttgatcaatt gaaccggcgg gaggcgggat aatcttcacg
     3541 accccttcaa gcacttcctt tacccctcgt ccatctttgg cactgactaa aaatgcctct
     3601 tcactcgaca accccaacac atctcgaatt tggactttga ccccttcgac atcggcgctc
     3661 ggcaaatcaa ttttattgat gacaggaata atcactaaat cattcgaaat cgccagatag
     3721 gcattggcga tcgtctgggc ctcaacgcct tgggtggcat cgattaacag aagtgctcct
     3781 tcacaggcag ccaggctgcg tgatacctca taggcaaagt ccacatgtcc aggggtatca
     3841 atgaggttaa actgatattc ctgtccatcc gaactccgaa accgtgctgt gactgcatgc
     3901 gctttgattg taatgccgcg ctcacgctcc aggtccatgg catctagata ttgcgcacgg
     3961 gcttcacgtg gagaaacggc accggtgatc tccaaaaacc ggtcggcaag agtggatttg
     4021 ccgtggtcga tatgagcaat aattgaaaaa ttgcggatgt gggtttgagg aaaggcttta
     4081 ctcattcgaa gtgactatga ttgtaatgag accctaaaag gttgtcaaaa ttcaatggaa
     4141 tccgctaggt tatttcctta aaaacgacca tgactctcaa gacgacccgc acagcattag
     4201 aaaaagacga ttgcgagtac ctgtggcacc cttttaccca aatgcaggaa tgggaacgtc
     4261 agactcctct tatcatcaaa cgcggaaaag gttcgtatgt ctatgatatg gatggcaatg
     4321 cctatcttga tgcgacggca tccatctggg tcaatattca tggacatcga catcctcaca
     4381 ttgatgaggc cattcgtcat caacttaccc aggtggctca tacgacgttg ctgggacttt
     4441 ccaacccacc agccattcaa ttggcgaagg cccttattcg cctggctccc aaagggttac
     4501 aaaaagtctt ctattccgac aacggctcca ccgccgttga agttgcggca aaaatggcca
     4561 ttcagtattg gcaacaatgt ccgaatcccc aaccacgaaa aactcggttt atccatcttg
     4621 gcatgtccta tcacggggac acggtgggag gaatgagcct cagcggagtc gaactcttcc
     4681 gcagatcctt ttctccgctc ctctttgcaa gccatgacgt ggagcccccg tattgctatc
     4741 gatgtcccct
//
LOCUS       DATIAL010000178        12337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_463025, whole genome shotgun sequence.
ACCESSION   DATIAL010000178 DATIAL010000000
VERSION     DATIAL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463025"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="VLA60_05525"
     CDS             <1..2355
                     /locus_tag="VLA60_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08852.1"
                     /translation="GFSYEQSVRPFTMLEKKTKKGLPVPMNTVLKGKAKKGDSSIKVK
                     NAAQYHVNTELLVGADNVKGNEIRRIKSIKGDTISFVRPLQHDHPAGDIVTVEFVRSR
                     LWADADVGTVFWHDHAFGATTWPHGGFGTLIVEPVGSTYHDPKTGKPIRSGPIADIHT
                     VEPVGYGVNGSFRELMVQVHDTVPHTVNIVTAGNPPGQPVEVALEAGKTVSFMMPEKI
                     AMTPMPFLNGGTHTTGSGLNFRAEPIAQRLAVNPDASKIFSSAEHGDPDTPLLRAYVG
                     DTVVFRLLHTLMNETMTWTLSGHTFLSERYAGDANRKNSIHVGIAERYDLVVPTAGGP
                     RLQPGDYIHFNGRSSKFSEGGWGILRVLDKETADLKPLPAGYSGRNEIPKPLPVCPEE
                     APVKSFNVVAMDFPGMSFNPSAPESIEIDFERTIALRNPEGKIYVLEDEVTKVAGDYH
                     PMPLTIRANVGDCIKVNLKNKMKESRASFSAISLAFNPKDSLGANVGNNPGDQTIAPG
                     ESKTYTYYADPFNGEISSLVWDWGNVMLNPRNGLFGAVIIGPKGSQYRDPKSGADISL
                     KNSWEADVIVDQTIPGQEHRRNYRDVALHFQDEDNIIGTSFMPYVQNVAGLTGVNYRS
                     EPYLHREEAGCTLGRMFQPCEVDNPQDPVTPIIQAHAGDPVRIHVFGANSEQNGMFSV
                     EKHEWPIEPFMPGADEISVVEFAGSETIDVFIPSAGGRDMLAADYVWNNQRLPYSQSG
                     QWGYLRVLPKNDQRILPLEGGASDVKQASIDVVETGRVLPVAAK"
     gene            2835..3776
                     /locus_tag="VLA60_05530"
     CDS             2835..3776
                     /locus_tag="VLA60_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF08853.1"
                     /translation="MRESQSRKYLVVMGLMVVGFTLMSGMIAWALDVSDIRPEWTEEG
                     KRLALQRTQWPTKGKRVVIPAGWFTMGSNKKIDRNAYRPEMPQRQVYLDAFEIDAYEV
                     TALEYLRFVVDTGRPPLPDWKFDGGNFQESMAHHPVMHVTWYEADAYCRWAGKRLPTE
                     AEWEKAARGTDGRIYPWGNTPAGLTRANFGRSGISGPVRDRPERLLLYPPIISVDKYE
                     IGISPYNVFQMAGNVAEWVNDWYDPAYYQTAPDRNPQGPSTGEHRVFRGGGWMDSTPT
                     VRTAQRNGTAPETQANWLGFRCAAGLKDDLTHSIPTP"
     gene            4143..5060
                     /locus_tag="VLA60_05535"
     CDS             4143..5060
                     /locus_tag="VLA60_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSF08854.1"
                     /translation="MRMRVMVIVMFLMNVSSLGLAYQESPVSNGGAIQGHVRILGERP
                     RPMAFNLVTIPDAVYCGRISTGTGWRIVEDFIIGPQGSLKDAVVMVKGVEKGKPFSLP
                     KVEVEAIDCDFLPFVNVLKDQDEITVINMDPVEHDIQGYETARERGARVLFNRPLPMN
                     PFHNVVGMFGKNVHTHLPGKPMIEKIHLRKGRDVFVMQCGFHPYMFSWGLVVDNPYYS
                     ISKEDGAYEIRDIPAGEYQVTVWHPGMKTYLEKTVRVESGKTLTLDFQYESPQGRRSV
                     HEMQENPHFGLEMLGEGVEIIPSLRRQVP"
     gene            5076..5996
                     /locus_tag="VLA60_05540"
     CDS             5076..5996
                     /locus_tag="VLA60_05540"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSF08855.1"
                     /translation="MNKRRVVEIIVISLLWITSSTAFAYEVIDVTDGATIVGTVTLKG
                     IPPSPKAYNLVTFPDPEYCGRISDGQGWRLLKDFVVNDDNQLQGVVMVVEDVKAGKPF
                     SLSIPKVEARDCQFLPFTTVVRNEHGIEVVNMDPVMHDIQAYETSLSQGTRVLFNSPL
                     PFNPRHQRENIHATHEHLPGQSLVHQFQLSKGRKTFVMQCGFHAYMESWAVAVDNPYF
                     SFTNERGEYVISSLPPGSYRIRAWHPSVKHEQVYEFSVEPNQTVRRDFSLESPVGRWT
                     AHTIRTPPRFTAAALGHPVLIEPLVEHQRP"
     gene            6164..6667
                     /locus_tag="VLA60_05545"
     CDS             6164..6667
                     /locus_tag="VLA60_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08856.1"
                     /translation="MWKTVGRIANAQLFVKNDRYRIEPAGGIRTELGYASVLIIRLDE
                     QKTWYVFSQPRMIVSVPLTLDYLLPFSVKLEGETSRTVIGESMVGDQPALLYDVEVKA
                     RSGQLERYFEWVDPQREILLKLLSQDRDWFVEYHQVVLSSQPDYYFEAPLGYRIIEAQ
                     EAPVPRG"
     gene            6708..8051
                     /locus_tag="VLA60_05550"
     CDS             6708..8051
                     /locus_tag="VLA60_05550"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08857.1"
                     /translation="MNRINAVLVLGVLWALSGSVLAEEAHDSTLVQAQKAYDQGQFQQ
                     VVDLVKPLYKESAPLSQASRLYILALASLGKTPEAIESYEKAIAGEKREDEPFLRQVA
                     IASILPVRSDMREQMRGAAYTALKEIESDKAVEYLEKGLTDETGMIRALVAEALGQRK
                     AGQRSKAFREALKDGAGLVRATVLIGLGQSGDRSLVPLIRPSLNDEQHLVQIAAARAL
                     YELGYKEYWARLEQGATSEEGYERGAAIRAFGELGDRRAIPILEKTISDSQPSIRAAA
                     IASLGKLQAPESLPTLKAVLFDRIPAVRSVAAFSMGYYQQHQVLTPLTRALADSNSGV
                     QAAAVASLLRSGAPYSVVKDTVQHLLNDQNPAIRSGAVKAIGNGKGPDVLNVLILALN
                     DPLPRPRIGASRALGRIGDRTLLPTLKRLLRDTDEAVRVTAAAAIVRILDKEIGL"
     gene            8264..10132
                     /locus_tag="VLA60_05555"
     CDS             8264..10132
                     /locus_tag="VLA60_05555"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HSF08858.1"
                     /translation="MVAGLSVLLLVSPLHAEIRPHGESVDHPIVQWHEWGNEAFGRAQ
                     AEDKLIILDLTAVWCHACHVMDQTTYANHRIVHLLNTKFIPVRVDTDQRPDLDARYRA
                     GGWPTTNVLLPTGEILFQANALGSEEMEAMLLEVQSTYVTDKAALLKEASQLWERVQE
                     QVGAGPSKANVLQTAMVKQSVEMMKAQFDPVNGGFRDAPKFFEPEAIQMVLSYGFFED
                     DPELLKIGLDTLKKQVGLLDPVWGGFYRYAEQADWSQPHFEKMLSIQALNLRNYVEAF
                     QLTGDLQFKRIAVALIEYVSRFLTDPQTGLMYESQDADVRGTEGIVSMSGAEYYSLNE
                     NKRLAIGIPYVDQRIFTGSNALMAWAYLHASVVLEQVEIGDLALRMLSRLFEKRFDEN
                     KGLAHGEMGDGPNLYGLLSDHTLFGQALLEAFRVTGRSQFLQNAEALAEVNRRILQDS
                     ENGGFFDHPQMPGKLGLLNFPTKPVKENLQAVLWYLDLFHLTEKQAYRSIAESTLQAM
                     VMSRQPLPLALSGLAIDQWFRFPIHIAVVGNFDDVMTKVLMVEGQRMFCPGKIVKRFD
                     PKEGPPKWGDIIFPYDERPVAFVCTDRMCSAPVFHAEDMKKSVADMMAVLRDPVQK"
     gene            10425..11219
                     /locus_tag="VLA60_05560"
     CDS             10425..11219
                     /locus_tag="VLA60_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSF08859.1"
                     /translation="MRNRQSLTMSFLVGCILVAAPLVAQAGGTIKGKVTFTGAVPPPK
                     EFAFSKFPNVDFCKKNESKSADGNTRLLKEVEVDGSKGLKNAVVAVTDIEDKGFLGKA
                     DKEAPQVVEAELCEFIPYTGVAVSKGLFKVVNNDADPSDPKSAEGVLHNPHSFDQLGA
                     KSSTEFNIGLAKKGDSLEKSVKLKMAKKGSVLRLQCDQHEFMQGWYLPVTNAHYSVAG
                     ADGTFEIKDVPAGKHKVKAWHPIAGEVEADVDVKDGGTVEANFEIK"
     gene            11408..>12337
                     /locus_tag="VLA60_05565"
     CDS             11408..>12337
                     /locus_tag="VLA60_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSF08860.1"
                     /translation="MIRESSLADIFQIGYYWETKILLTAVKLDLFTALKGESLTVNEV
                     ADSLKLNPRALELVMNALVAMRVLTKDEKLYTNTPVAERHLVQSSSEYVGHLLLLHDA
                     EWSHWGQLEQTVKTGRSPVTQHVFETNPELGAHVLTVLDRIGQGSGQGLAKSVPIHGT
                     VRMLDIGGGAGTNAIAFCLEHPGLSATVFDLPQTLKVTKDHVDRAGLGDRIHLQGGNF
                     HVDAFQGSYDLALMSDILHYQDGSTNAALVKKVFACLTEGGRLIIKDRFLDPAKTSPA
                     WTTAFAVHILVNTECGECFTIQDSRQWMEQAGFR"
BASE COUNT         3060 a         2660 c         3373 g         3244 t
ORIGIN      
        1 ggtttttcct acgaacagtc tgtccgtccg tttaccatgc tggagaaaaa gaccaaaaaa
       61 ggcttgcctg tgccgatgaa tactgtctta aagggcaagg caaaaaaggg cgattccagc
      121 attaaggtta aaaatgcggc gcaataccat gttaatacgg aactactggt cggtgccgat
      181 aatgtcaaag gcaacgagat tcgacgtatt aaatccatta aaggggatac catttcattt
      241 gttcgaccct tgcagcatga tcatcccgca ggggatatcg tgaccgtgga atttgttcgg
      301 tcgcgactct gggcggatgc tgacgtggga acggtgtttt ggcatgacca tgcctttggt
      361 gcgaccacgt ggcctcatgg cggattcggg accttgattg tggaaccagt aggctccacc
      421 tatcatgatc cgaagaccgg gaagccaatc aggagcggtc cgatcgcgga catccatacc
      481 gtagaaccgg ttggatacgg tgtgaacggt agttttcgtg aactcatggt ccaggttcat
      541 gacacggtgc ctcatacggt gaatattgtg acagctggta acccgcctgg gcagccggtc
      601 gaagtagccc tggaggccgg gaaaacggtg tcatttatga tgccggaaaa aattgctatg
      661 acccccatgc ctttcctcaa cggggggacg catacgactg ggagtggcct caatttcagg
      721 gctgaaccga ttgcccaacg attggccgtg aatcccgatg cctcaaaaat attcagtagc
      781 gctgaacatg gagatccaga tacgccatta ttgcgggcgt acgtgggtga tactgtggtg
      841 tttcgtcttt tgcataccct catgaatgaa acaatgacct ggaccttatc agggcacacg
      901 tttttgagtg agcgatatgc gggggatgcc aatagaaaaa attcaattca tgttggtatc
      961 gccgagcgct atgatttagt cgttccaaca gccggcggtc ctcgtctgca acctggagac
     1021 tatattcatt tcaatggacg atcttccaaa ttctccgagg gaggttgggg aatcctacgg
     1081 gttttggata aggagacggc tgacctgaaa ccgctcccag cagggtattc aggtcgaaat
     1141 gagattccca agccccttcc tgtttgtcct gaagaggctc cggtgaaatc atttaatgtg
     1201 gtcgccatgg actttcctgg catgagtttt aatcctagtg ctccggaatc cattgagatt
     1261 gactttgaga ggaccatcgc gttgagaaat cctgagggca aaatctacgt ccttgaagat
     1321 gaagtcacta aagtggcagg ggattaccac ccgatgccat tgactattcg agcaaacgtg
     1381 ggagattgca tcaaagtgaa tctcaaaaat aaaatgaagg agagccgtgc atccttctcc
     1441 gcaatttcac tggcctttaa tccgaaggat tcattggggg ccaacgtcgg gaataacccc
     1501 ggggatcaaa cgattgcccc aggtgagtca aagacgtata cctattatgc tgatcctttt
     1561 aacggggaga tttcatctct ggtatgggac tggggaaatg tgatgttaaa cccgagaaac
     1621 ggtttgttcg gcgcggtcat catcgggccg aaagggtcac aataccggga tccaaagtcc
     1681 ggtgccgaca tttcccttaa aaatagctgg gaagcggatg tgattgttga tcagaccatt
     1741 cctggtcaag aacaccgccg aaattaccgt gatgtagctt tacactttca agatgaagac
     1801 aacatcattg gtaccagctt tatgccgtat gtccagaatg tggccgggct caccggtgtc
     1861 aattatcgct ctgaacccta tcttcataga gaagaggccg gttgtacact tggacgcatg
     1921 ttccaaccct gtgaggtgga taatccacaa gatccggtta ctcccattat ccaggcacat
     1981 gcaggggatc ctgtgagaat tcatgtgttt ggtgccaata gcgaacaaaa cgggatgttt
     2041 agtgtggaaa agcatgaatg gccgattgaa cccttcatgc cgggagccga cgaaatcagt
     2101 gtggtcgaat tcgctggttc ggaaacgatc gatgtgttta ttccgtccgc tggagggcgt
     2161 gatatgcttg ccgcagatta tgtctggaat aatcagcgcc taccttactc ccaatcgggt
     2221 cagtggggtt atttaagggt cctacccaaa aatgaccaac ggatccttcc tctggaaggg
     2281 ggtgccagtg atgtgaaaca agcttcgatc gacgtggtgg aaactggccg ggttttgccg
     2341 gtggcagcca aatagccatt ttcaccatca aggtttttac gaatggggga ttcgcaagaa
     2401 tcccccattt ttttatttag gtttattaaa atggatgtct tcggttttct aaagctaggc
     2461 aactcaaagt tccattttga ggaaatttga aaatggcaac agaacaattg ttataatatt
     2521 tttttaggat gactttctcg tatatggaat atcttactta tccctttttg aatcctactg
     2581 actaccgact aaaccgattc ggcaatgagt aaaggcggat tagggtggta gtctcagacg
     2641 ccttgacctc actgaaaggt gaagctacga tttggtccat taaacgacca tactgaaaca
     2701 caaatattgg ggttcccagc aaaatgcctt cgtccagaac gatacgaaga gatcagtgct
     2761 tgttgttggg atggtgaatt ctccaatttt tccatcagtg gcgtcttagt cgtcggatta
     2821 atttggaggg gtctatgcgt gaaagccaaa gcagaaagta tcttgtggta atgggcttga
     2881 tggtggtcgg gtttaccctc atgtccggga tgatagcgtg ggcactggac gtgtctgata
     2941 tacggccgga gtggaccgag gagggcaaaa ggttggcttt gcagcggacg caatggccta
     3001 ccaaaggaaa gagagtggtg attccagccg gttggtttac gatgggaagt aataagaaaa
     3061 ttgataggaa tgcctaccgg cctgagatgc ctcaacgcca ggtgtatctg gatgcttttg
     3121 agattgatgc ctatgaagta accgctttgg aatatttgcg ttttgtcgtg gacacgggcc
     3181 gtccgccatt gccggattgg aaatttgacg ggggaaactt tcaggagagt atggcccatc
     3241 atcccgtgat gcatgtcacc tggtatgaag ctgatgccta ttgccggtgg gccggcaagc
     3301 ggctccctac ggaagcagaa tgggaaaaag cggccagggg cacggatgga cgaatttatc
     3361 cgtggggaaa tacgccggca ggtctgacgc gagccaattt tgggagatcg ggtatatccg
     3421 gtccggtccg tgatcgcccc gagcggttgc ttctttatcc acctattatt tctgttgata
     3481 aatatgaaat cggtatcagt ccctataacg tgttccagat ggcagggaat gtcgcagaat
     3541 gggtgaatga ttggtatgat ccggcctatt atcagacggc tccggaccgg aatcctcaag
     3601 gcccgtctac cggtgaacat cgggtatttc gtggaggtgg ctggatggac agtactccga
     3661 ccgtacggac cgcccagcgc aatggaaccg ctcctgagac acaggccaac tggcttggct
     3721 ttcggtgtgc tgctggattg aaagacgacc ttacccattc gatcccaact ccttgaatgt
     3781 aagaagtgga tgtattcttt gtttctgagc tggttttcat tccttctcac aaaattattt
     3841 attcttgtaa accctttctc ctgaacgaag ttccttgcac gagtttcaga gggtttgagt
     3901 tatcaatgag cttagagttg aagttccgat gacccaaatg tggtagggtg atgtgagagg
     3961 agtggtttag acaaggagag ttctccatac tcttttgagt tgttcggata aaccttttaa
     4021 acgcttgagt gtgccgtagc atgtgactcc tggtgtttcc cgcccatagt attttgggga
     4081 agcaaacaga tattacacat agctatttat tggtgggcag tgataggaag gagggaattg
     4141 gaatgaggat gagggtcatg gtgatcgtga tgttcctgat gaatgtgtca tccttggggt
     4201 tggcctatca ggagagcccg gtcagcaatg gaggggcaat tcaaggccat gttcgcattc
     4261 ttggggaacg cccaaggccg atggcgttta atctcgtcac gatcccggat gccgtatatt
     4321 gtggaagaat ttctaccgga accgggtggc gcattgttga ggattttatt attgggcctc
     4381 aggggtcgct taaggatgcg gtggtgatgg tgaaaggggt agaaaaaggc aaaccgtttt
     4441 ctttgccaaa agtagaggtt gaagccattg attgtgattt tttaccattt gtcaatgtgt
     4501 taaaggacca ggatgagata accgtgatca atatggatcc ggtagaacat gatatccagg
     4561 gatatgaaac ggccagagaa cgaggggcca gggtcttgtt taatcgtccc ttgcccatga
     4621 acccctttca taatgtggtt gggatgtttg gaaaaaacgt ccatacccat ctgcctggaa
     4681 aaccgatgat tgaaaaaatt catttgcgaa aaggacggga tgtttttgtc atgcaatgtg
     4741 gttttcatcc ctatatgttt tcatggggat tagtggtgga taacccctat tattcgattt
     4801 ccaaagagga tggagcctac gaaatccgcg atatacccgc aggagaatat caagtgactg
     4861 tctggcaccc tggtatgaaa acctatttgg aaaaaactgt tcgtgtggaa tcgggaaaaa
     4921 cactgacatt ggatttccaa tatgagtctc cccaagggcg gagaagcgtg catgaaatgc
     4981 aggagaatcc acattttgga ctggagatgc ttggagaggg cgttgagatt atcccgtcgt
     5041 tacgacggca agttccctga gggcatgagg tgatcatgaa taaacgtcga gtagtagaaa
     5101 ttatcgtcat aagtctgctc tggataacca gttcgaccgc tttcgcttat gaagtgattg
     5161 acgtaacgga cggggccact atagtcggca cggtgaccct caagggcatt ccgccttctc
     5221 ctaaagcgta taacctggtg acgttccctg atccggagta ttgcggccgc atttctgatg
     5281 gccagggctg gcggcttctt aaggatttcg tggtgaatga tgacaatcaa cttcaaggag
     5341 tcgtgatggt ggttgaagac gttaaagccg ggaaaccatt ttccctttct attccgaaag
     5401 tggaagctcg ggattgtcaa tttctgccct ttacgacggt ggtgcgtaat gagcatggaa
     5461 ttgaagtggt gaatatggat cccgtcatgc atgatattca ggcctatgag acgtcgctga
     5521 gccaagggac acgggtgtta tttaattctc ctctgccctt taatcctcga catcaacgtg
     5581 agaatatcca tgcgacgcat gagcatctgc cggggcaatc tttggttcat caatttcaac
     5641 tgagcaaagg acgaaaaacc tttgtgatgc aatgcgggtt ccacgcttac atggaaagct
     5701 gggcggttgc ggtggataat ccctatttca gctttactaa tgaaaggggg gagtatgtca
     5761 tttcgtcact cccgcctgga agttatcgca ttcgggcctg gcatccaagt gtcaaacatg
     5821 agcaggttta tgaattttcc gtggaaccaa accaaaccgt gcggagagat ttttccttgg
     5881 aatctcccgt gggtcgttgg acagcgcata ccatccggac gccgccgcga tttactgccg
     5941 cggccttggg ccaccctgtt ttgatagagc ctctggttga acaccaaagg ccatgaaatg
     6001 gatagacttt tccctccttc tctcccgaac atgtgaaaag aaatttgcag gcctcgttgc
     6061 ttgtgccatt ttcttaatgg tggctgggtt tccattagcc tgggtctttg ccggctcttc
     6121 caatttccca caccagggtc ccacggaatt ttcggcgaga ctgatgtgga aaaccgtcgg
     6181 acgtatcgct aatgcccagt tgtttgtgaa aaatgatcgg tatcgcattg aaccggcagg
     6241 gggaatacga acagagttag gctatgcgag tgtcctcatt attcgattgg atgaacaaaa
     6301 aacctggtat gtgttttcgc aaccccgaat gattgtgagc gttcccttaa ctttggatta
     6361 tttgttacca ttttctgtca agttggaagg agagacgagt cgaacggtaa ttggggaatc
     6421 catggtaggc gatcaaccag ccctgctcta tgatgtggag gtcaaagctc ggtctggcca
     6481 actggaacga tatttcgaat gggtggatcc tcaacgagag attctcttga agctcttaag
     6541 tcaggatcgg gattggtttg tggaatatca tcaagtggtg ctttcttcgc aacccgatta
     6601 ttactttgaa gcgcctttgg gctatcgaat tattgaggct caggaagccc cggttccgag
     6661 agggtagaaa tatgatatgg ctaggagatg ggggtaaaca ataaaggatg aatcgtatta
     6721 acgctgtgct cgttctcggt gttctttggg ctctctcagg ttcggttttg gcggaagagg
     6781 cgcatgattc aacccttgtt caggcgcaaa aagcctacga tcagggtcaa tttcaacagg
     6841 ttgttgacct tgttaagccc ttatacaaag aaagtgcgcc cctctcgcag gcttctcgtc
     6901 tatacatcct tgcattagcc agcttgggga agactcccga agccattgag tcctatgaga
     6961 aagccatcgc cggggaaaag cgggaagatg aaccgttctt acgccaggtt gccattgcca
     7021 gtatattgcc tgtccgttcc gatatgcggg agcaaatgcg aggcgctgcc tatacggcgt
     7081 taaaggaaat cgaatctgat aaggccgtgg aatatttaga aaaaggctta actgatgaaa
     7141 ccggcatgat tagagcattg gtggcagaag ctttagggca aagaaaagcc gggcagcgtt
     7201 ccaaagcgtt tcgtgaagca ttgaaagacg gagccggttt ggtgcgggca accgttttga
     7261 ttgggctagg acaatccggc gacaggagtc tcgtgcccct cattagacca tcattgaacg
     7321 atgagcagca tcttgtccaa atcgccgcag caagagcatt atatgagctg ggttacaagg
     7381 aatactgggc tcgtttggaa caaggtgcga caagcgagga aggatatgaa cggggcgcgg
     7441 ctatccgtgc atttggtgaa ttaggggatc gtcgtgcgat accgatcttg gaaaagacta
     7501 tttccgattc tcaaccctct atccgtgccg cggccattgc atccttggga aaattgcaag
     7561 ctcctgaatc tttgccgaca ttgaaggcag ttttgtttga tcgtattcct gcggtgcgga
     7621 gtgtggcggc ttttagcatg gggtattacc aacaacatca agtgttaacc ccgttaacca
     7681 gggcgttggc cgattcgaat tccggtgtgc aagcggcggc tgtggcctca ttgctacggt
     7741 caggagcgcc ttattcagtg gtcaaggaca ccgttcagca tcttctcaat gaccaaaatc
     7801 cggccattcg gtcgggtgcg gttaaggcga ttggaaatgg aaaaggtcct gacgtactca
     7861 atgtgcttat attggcactc aatgatccat tgcctcggcc caggattggg gcctcgcgcg
     7921 cattgggtcg tataggcgat cgcacgctcc tcccgacttt aaagcgcctc ctgcgagata
     7981 ctgatgaagc ggtgcgggtg actgccgccg ccgccattgt gaggattttg gataaggaga
     8041 tcggtcttta accggtttgt ttgcggccct aagagaggtc ttcctgaaga tgggcaggta
     8101 tgaccggata aagggtgtga acatgtttcc ccaaattgtg cagtatatta ggcactgctc
     8161 ccctctcgat caattttcgc gcacgatcat caagttcgta tttgtcccgg tgactgtggg
     8221 tatgaatcac tcatgccgaa tccgggaaag ttgcagatgg tggatggtgg ccggactcag
     8281 cgtgttgctt ctcgtcagtc cccttcatgc tgaaatacgg cctcatggcg agtctgtcga
     8341 ccatccaatt gtccaatggc atgaatgggg aaatgaagcc ttcggacgtg cgcaggccga
     8401 agataaatta attattttgg atctcaccgc tgtatggtgt catgcttgcc atgtgatgga
     8461 tcagacgact tacgccaatc atcgtatcgt acacctgctc aacaccaaat ttattcctgt
     8521 ccgggtcgat actgatcagc gtcccgattt agatgctcgt taccgagcgg gaggatggcc
     8581 gacgaccaat gtcctcttgc cgaccggaga aattcttttt caagcgaatg ccttgggttc
     8641 tgaggaaatg gaagccatgc ttcttgaggt gcagtctacc tatgtcaccg acaaggctgc
     8701 gttgctcaag gaagcctctc agttatggga gcgggtacaa gagcaggtgg gagccggtcc
     8761 ttccaaagcg aatgtgcttc agaccgccat ggtcaagcaa agtgtggaga tgatgaaagc
     8821 acaatttgac cccgtcaatg gcgggtttcg ggatgcccca aaattctttg aacccgaagc
     8881 catccagatg gtcttgtctt atggtttctt tgaggatgat ccagagttgc tcaagatagg
     8941 attggatacc ttaaaaaaac aagtgggctt acttgatccg gtatggggcg gattttaccg
     9001 ttatgctgaa caagcagatt ggagccaacc gcattttgaa aagatgttgt cgattcaggc
     9061 tctgaatctt cgcaattacg ttgaagcctt ccaactcacc ggagatcttc agtttaaacg
     9121 catagccgtg gcgcttattg aatatgtttc gcggttttta accgatccac aaacggggtt
     9181 gatgtacgaa agtcaggacg ccgacgtcag aggaactgaa gggatagtgt ccatgtctgg
     9241 agccgagtac tattctctca atgagaacaa gcgattggcc atcgggatcc cgtatgtgga
     9301 ccaacgaatt ttcacaggga gtaatgcgtt gatggcatgg gcctatttgc atgcctccgt
     9361 ggtgttggaa caggtcgaga taggggactt ggctcttcgg atgttgagcc ggttatttga
     9421 gaaacgtttt gacgagaata aggggctcgc tcatggcgag atgggagacg ggcccaatct
     9481 ttacgggtta ttatcggatc acaccctgtt tggccaagcg ttacttgagg cctttagagt
     9541 gaccgggcga tctcaatttt tgcaaaatgc tgaagccctt gcagaagtga accgccggat
     9601 attacaagat tccgagaatg ggggattttt tgatcatcca cagatgccag gtaaattggg
     9661 tcttctgaac ttcccaacca aaccggtgaa ggagaatctc caagccgtcc tctggtacct
     9721 cgatcttttt catttgacgg agaagcaggc atatcgctcg atcgctgaga gcacgttgca
     9781 ggccatggtc atgtcccggc agcctcttcc cctcgcattg tcgggattgg ctatcgatca
     9841 atggtttcga tttccgattc atattgcggt ggtgggaaat tttgacgatg tcatgaccaa
     9901 agtcctgatg gtggaaggcc agcgaatgtt ctgccctgga aaaattgtga aaaggtttga
     9961 ccccaaggag ggaccgccga aatggggtga cattattttt ccctatgacg aacgccccgt
    10021 agcgtttgtc tgtactgacc ggatgtgttc ggctccggtt tttcatgcag aggacatgaa
    10081 aaagagtgtg gctgacatga tggccgtact cagggaccct gttcaaaaat gagcgatgct
    10141 gcctaattgc cagtctgtcg atttttagga aattatcgtg tgtttaaaac ttttcacttt
    10201 tccgcgaact aaaaggccta aggtttagca caaaggacct agttcttgtt gggtttcttg
    10261 acacgttttt tttattcctg atatatacgg aagtgggtgc tgtcggacga tccatttcaa
    10321 gatcagcttt tttatacctg gccggggtaa aaggggttga tcttatcaag ttaggttgtt
    10381 ttcccacgtc aactcaattt tattcttaac taggaggaag aatcatgagg aatcgtcagt
    10441 ctttgactat gtctttttta gtgggctgca ttcttgttgc ggccccatta gtcgcccagg
    10501 ctggaggaac tattaaaggg aaagtcacgt ttactggagc agttccccca ccgaaagaat
    10561 tcgcgttttc aaagtttccc aatgtggatt tttgcaaaaa aaatgagagc aaaagtgcgg
    10621 atgggaatac ccggttgttg aaggaagtgg aagtggatgg aagcaagggg ttaaagaacg
    10681 cggtggtagc ggttactgat attgaagaca aaggattttt aggcaaagcc gacaaggagg
    10741 ccccgcaagt ggtggaggct gaattatgcg agtttattcc gtatacgggt gtggccgtca
    10801 gtaaaggact gtttaaggtc gtcaataacg atgccgatcc cagcgatcct aaatcggctg
    10861 aaggtgtgtt gcataacccg catagcttcg atcaattggg tgccaagtcc agcacggagt
    10921 tcaatatcgg tctggccaaa aaaggtgatt ctttagaaaa aagcgtcaaa ttgaagatgg
    10981 cgaaaaaggg ttctgtattg cggttgcaat gtgaccaaca tgaatttatg caaggctggt
    11041 atttgccggt gaccaatgcg cattatagcg tggccggtgc cgacggaacc tttgaaatca
    11101 aggacgtgcc tgccggcaag cacaaagtaa aggcctggca tcctattgcc ggggaagtgg
    11161 aagctgatgt ggatgtgaag gacgggggaa ccgtcgaagc caatttcgag attaaataag
    11221 tcttctcttt cgattgaggc atagcacttc ttcaagggca actagccatg agctagttgc
    11281 ccttgttttt tgtggagaag gttagactat ttttctgatc gttcagcatt tgacctcgca
    11341 aaatgacccc gttaagatcg acatcgaaaa tgatttttca tgttgccagt tgagtttgag
    11401 gccccatgtg attcgtgaat catctctcgc agatattttc cagatagggt actactggga
    11461 aaccaaaatt ctcctcaccg ctgttaagct tgatctcttt accgcactca agggagagtc
    11521 gctcacggtc aatgaggtcg ccgattccct gaagctcaat ccacgggcct tggagttggt
    11581 catgaacgca ctagttgcga tgcgtgtatt aacgaaggat gagaagctat ataccaatac
    11641 tcctgtggct gaacgacatt tggtgcaatc aagctcagag tatgtgggcc atttattact
    11701 gttgcatgac gctgagtgga gtcattgggg gcaattggaa cagaccgtaa aaaccgggcg
    11761 gtcacctgtg acgcaacatg tttttgaaac gaatccggaa ctgggagctc atgtgctcac
    11821 cgtcctggat cgaatcgggc agggaagcgg gcagggattg gcgaaaagtg tccccattca
    11881 tggaacggta cggatgctag atattggtgg tggggccgga accaatgcca ttgccttttg
    11941 cctggagcat cccggattgt ccgccacggt ttttgatctc ccgcaaacct tgaaggtgac
    12001 caaagaccat gtagatcgtg cgggattagg ggatcgtatc cacttgcaag gcggcaattt
    12061 tcatgtcgat gcatttcagg ggtcgtatga cctggccctc atgtcggata ttctacatta
    12121 tcaggatggc agtaccaatg cggcactggt gaagaaagta tttgcctgtt tgacagaggg
    12181 ggggcgactg atcatcaaag accggttctt ggatccggct aaaacgagcc ctgcttggac
    12241 gacagccttt gctgttcata ttttagtgaa cacagaatgt ggcgaatgtt ttacgattca
    12301 agacagtcgc caatggatgg agcaagccgg ctttcga
//
LOCUS       DATIAL010000179         7077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1215653, whole genome shotgun sequence.
ACCESSION   DATIAL010000179 DATIAL010000000
VERSION     DATIAL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215653"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VLA60_05570"
     CDS             <1..360
                     /locus_tag="VLA60_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF08861.1"
                     /translation="MEAQEVDETLLQAKGAVVLSLDDLGSNDEDSHPILDALADRDQP
                     SPLETLVSEDARQALVDAIERLPERQRLVLTLYYFEELTMKEIGVVLHVTESRICQLH
                     AQAMIRLKALLHTRLGP"
     gene            587..1786
                     /locus_tag="VLA60_05575"
     CDS             587..1786
                     /locus_tag="VLA60_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HSF08862.1"
                     /translation="MTTENVLDMKAPFGDPLYTILIVDDEKVVRMVAKKRLAKLPCRL
                     LEAGSGEEALAMLDQEPVDLILSDWVMPGLDGPGLCEAIKQDERYRTIHFILMTALDH
                     PTQIAEGLSRGADDFLSKTDSDHEIVARVCAGLRARQLMLYVEKSNRLLSQKQAELDS
                     ELRSASTFVRGLLPRTGEVIPGVRVEWEFLPSSHLGGDFFQIARWGDDHLGLMVLDMS
                     GHGIGPALRAVSLSLLFKGDHMQQLFPSYDPGEILTSLNQQYPMNDDGEYFTIWVGVW
                     QCSTRLLRYASAGHPGSIVVKTDGSSIVLGERSWPIGFSRNELYGTNSIFVNSGDRMY
                     LYSDGIYEVMNSQEEIWGKKRLQKALEEVAGQPMQSGLSRIIEQSRTWNAKGGFEDDV
                     ALLGVEF"
     gene            1886..2854
                     /locus_tag="VLA60_05580"
     CDS             1886..2854
                     /locus_tag="VLA60_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08863.1"
                     /translation="MTNTFSRILLVDDDASNLDVLVECLKDEPYELIQAHNGEEALAY
                     LRKDGQDFHAMVLDRMMPGMNGLDLLGHLKADERLQWLPVVMQTAAGSPQEIGEGIEA
                     GVFFYLIKPFDQRLLLRIVNAAVEEGSKWKRICRNLHQQSQIVELLQQGRFHVRTMEE
                     AYDLAFLLGRACPNSEKVVFGLNELLANGVEHGNLGIGFDEKTALQAKGTWEKEIQHR
                     AGLPEYVHKVVDVLFERCLDCIQVTITDQGSGFDWRKYEQINPDRILESHGRGIAMAK
                     ALSFSRIEYRGKGNQVVCVIKTDLSIGEEGSSLVSEPMAVHKSDRA"
     gene            3117..3413
                     /locus_tag="VLA60_05585"
     CDS             3117..3413
                     /locus_tag="VLA60_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF08864.1"
                     /translation="MEECQVTSMIPEEAILRPQGDLTIFEAAQFHADLVSLHQQQGPL
                     AIDLGGVICMDSSCVQLMVAATRSGRVTVQGYSNRIRDKFEQIGFAQFLPTQES"
     gene            3472..4608
                     /locus_tag="VLA60_05590"
     CDS             3472..4608
                     /locus_tag="VLA60_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF08865.1"
                     /translation="MNGDWMVWMGVVVAGAGIGYVLGARSRQMRIHRLEQQLQEGIQA
                     QRRWLENWKAFCQCLAPVFPVFVGQIKAVIQETGQAADGLIQRFQAISRNAREQVEET
                     EALLHMADGHGGKDDYTVDRILHDTQKTIEMFVKQVTQTTTVTLATVSVMEQALETTS
                     RISEVVEEVEFIADQTRLLALNAAIEAARAGEHGRGFAVVADEVTKLANRSAQASEQI
                     RTLATTVKGTTESAMSELQVLASLDLSETVEAQNQVLEMIKIMGQKNEALKKSVGHNS
                     GRTKELGKDIGQIVMSMQFQDITRQKLEHVYQPLERIHAPLQAVADDTDGLDMMPKVL
                     EEIRNLEHTYTMESERLTMQAARSGQGTVMVGTGSDEGDNITLF"
     gene            4765..5136
                     /locus_tag="VLA60_05595"
     CDS             4765..5136
                     /locus_tag="VLA60_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08866.1"
                     /translation="MGKTVLVVDDSVSMRQMVSFTLTGAGYEVVEAGDGKEAVDKLNG
                     GAKPNLVITDLNMPNMDGISLIKAIRGMAAHKFTPILMLTTESSDDKKKEGQSAGATG
                     WVVKPFNPEQMLATIKKVLPG"
     gene            5230..5598
                     /locus_tag="VLA60_05600"
     CDS             5230..5598
                     /locus_tag="VLA60_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HSF08867.1"
                     /translation="MKNWNLAMQLVTDVYQITKIFPEQERYGLTSQMRRAAVSIPSNI
                     AEGAARQTPKEFIQYLHVSQGSLSELDTQLDISKRLGWLQAKDVDELESAMNRLDKML
                     SGLIRHKNRHTSYASRLTKR"
     gene            5630..>7077
                     /locus_tag="VLA60_05605"
     CDS             5630..>7077
                     /locus_tag="VLA60_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HSF08868.1"
                     /translation="MAVDLSRFQESFFTESAEHVETIESGLLALEQRPNDLDLLNRIF
                     RGAHSIKGNAGMFHFTAIAELTHKMENILDDLRNEKMPVTPHVIDVLLRALDGLKSLL
                     DAAQGGEAANETAIHSLEGELEACQKGATAPAHEGSFAEAGPQGSPPQGATWRKIQID
                     WSALPELFQRGMDPAQIFKELHELGMVKVLHVRTDGLPALEALDPERCYLSWRVELET
                     DASISQIEEVFAFVRDGSELAIIDCAPKKPEPYKRVGDILVEEGVVTPEQIAESLAKQ
                     KPLGEILVEEKKASPQQVDKALQKQLQLKKNEVASIRVDTEKIDKLINLVGELVITQS
                     MITDLSEKFTLAQLPVLQERITQLERNTREIQERVMSIRMMPIGSAFHRFPRLVRDLA
                     GKSGKQIQLVMSGEETELDKTLIEAIGDPQVGVDAGEPARSIEELPLGEDAPDVRLGV
                     EVVPHAADERAGRFRELELGVIVGLDGGRLVVR"
BASE COUNT         1791 a         1547 c         2068 g         1671 t
ORIGIN      
        1 atggaggctc aagaggtgga cgagactctt ttgcaagcca aaggagccgt cgtattaagt
       61 ctcgatgatt tgggctccaa tgatgaagac tcccatccca tcctggatgc gctggcggat
      121 cgagaccaac cctcgccgtt ggaaactttg gtgtcggagg atgcccgcca ggcgttggtc
      181 gatgccattg agcgattgcc ggaacgtcaa cggttggtgc tgaccctcta ttattttgaa
      241 gagctgacca tgaaagaaat tggagtcgta ttgcacgtca cggaatcacg aatttgtcag
      301 ttacatgccc aagccatgat tcgtctgaag gcccttctcc atacccggct cggtccgtag
      361 aaaggcttaa ggggtgcggc ggcagatctc aggcgaaggc tgcggctttt tttccgccaa
      421 gcccgacatt gtcatcgggc atcgcccttc aattcatgaa caatatccta agcgtgaggc
      481 atgagtatgg tgaagtacgg cgatccgttt ttctcctaat ttcctgacac ttcacgtttc
      541 ccttgttatg caattcccgg atacatctgt gggttcaatg aagcagatga cgaccgaaaa
      601 tgtcctcgat atgaaagcgc ccttcggcga tcctctttat accattctca ttgtggatga
      661 tgaaaaggtt gtgcggatgg tcgccaagaa gcgtttggcc aaattgccgt gtcgattatt
      721 ggaagccggt agtggggaag aagctctggc catgctggat caggaaccgg tggatctcat
      781 cctatcggat tgggtcatgc ccggtcttga tggtcctgga ctttgtgaag cgattaaaca
      841 ggatgaacgc tatcgcacga tccattttat tctgatgacg gcattggacc acccgacaca
      901 aattgccgaa gggctcagcc ggggagccga tgattttctt tccaaaaccg attccgatca
      961 tgaaattgtc gccagagtgt gcgcgggcct tcgagcccga cagttgatgt tgtatgtgga
     1021 aaagtctaat cggttgttat cacaaaagca ggccgaatta gactctgaac tccgctcggc
     1081 ctccaccttt gtgcgaggtc tgcttccccg gaccggggaa gtgattcctg gagtgcgggt
     1141 ggagtgggaa tttttgccgt cttcccattt gggaggagat ttctttcaga tcgcccgatg
     1201 gggggacgac catctcggtc tcatggtgtt ggatatgtcg gggcatggga tcgggccggc
     1261 gttgcgggcc gtgtcgttat ctctgctttt caagggagac cacatgcaac aattgtttcc
     1321 ctcctacgat ccaggtgaga ttctgacgag tctgaaccaa caatatccca tgaacgatga
     1381 cggggagtac ttcacgatct gggttggggt gtggcaatgc tccacgcgcc ttcttcgata
     1441 tgcgagtgcg ggacatcccg gttccattgt tgtgaaaacc gacgggtcgt cgatagtgtt
     1501 gggcgagaga tcctggccta ttggtttttc gcgtaacgag ttgtatggaa ctaattccat
     1561 cttcgtaaat tccggtgacc ggatgtatct ctatagtgat gggatttatg aggtgatgaa
     1621 ttctcaggaa gagatctggg ggaaaaaaag gcttcagaag gcacttgagg aggttgccgg
     1681 tcagccgatg cagtcgggat tgtcccgtat tatcgagcag agccggacat ggaatgccaa
     1741 aggcgggttt gaggatgacg tcgcgttact tggagtggag ttttaggcgt gaggagatag
     1801 cagcaagata cttctttagc cttacgcttc atctctcacg ttccaccctg acacctcacg
     1861 attggctgtt gtcgaaatga caggtatgac aaacacattc tcccgaatcc tattggtgga
     1921 cgacgatgcg tccaatctgg atgttcttgt cgaatgtctc aaggatgaac cctatgaact
     1981 tattcaggca cataatgggg aagaggcatt ggcgtattta cggaaggatg gacaagattt
     2041 tcatgccatg gtgttggatc gaatgatgcc ggggatgaat ggtctagatc ttttagggca
     2101 tttgaaagcg gatgagcgtc tccaatggct accggttgtg atgcaaactg cggccggatc
     2161 tccgcaagaa ataggggaag ggattgaagc gggagtcttt ttttatctca tcaagccctt
     2221 tgaccagcgg cttttattgc ggattgtaaa cgccgcggtt gaggagggat cgaaatggaa
     2281 acggatatgc cgaaatcttc accaacagtc tcagattgtt gaattattgc agcaaggccg
     2341 gtttcacgtt cgaaccatgg aagaggccta tgatctggca ttcctattgg ggcgagcgtg
     2401 tcccaattca gaaaaagtcg tgttcgggtt gaatgaactg ttggcgaatg gcgtggagca
     2461 tggaaattta ggaatcgggt tcgatgaaaa aacggctctc caggcaaagg gaacgtggga
     2521 gaaagaaatt caacatcggg cggggctacc tgagtatgtt cataaggtgg tcgacgtgtt
     2581 gtttgaacgg tgcctggatt gcatccaggt aaccataacg gaccaaggtt cgggctttga
     2641 ctggcggaaa tatgaacaga taaacccgga tcgaattttg gaaagtcatg gaaggggtat
     2701 tgcgatggcc aaggccttaa gtttctcacg gatcgagtat cgagggaaag gcaatcaggt
     2761 cgtgtgtgtc atcaagaccg acttgtcgat cggtgaagaa gggagctctc ttgtatctga
     2821 acctatggcc gttcacaagt cggatcgggc gtgaggagtc attcgtgagg gaaaaacaat
     2881 tttacccgct cccaaaaatt tccctccgtt tccttactcc tcacctcagg ttatgggcat
     2941 cacaagaaaa agtggatcct tctaaagtaa ggtccattgg cctccgaaag gtctcgttgt
     3001 ctcctgttta ttccttgata ggccgaaggg ttctacctgg caatggaagt gtcaggctag
     3061 gcaggaaggg ttgttgaatg tccacgtttt ttccccccga aaaatgagag ggtaacatgg
     3121 aagaatgcca agtcacgtca atgattccgg aggaggcgat tctcaggcca caaggcgatc
     3181 tcaccatttt tgaagcggca caatttcacg ccgatttagt gtccttgcat caacaacaag
     3241 ggcctctcgc gattgattta ggcggggtga tatgtatgga ttcctcctgt gtgcagctga
     3301 tggtcgcagc gacccgctcc gggcgggtga ccgtgcaggg gtattccaat cgtattcgtg
     3361 acaagtttga gcaaattgga tttgcccagt ttcttccgac ccaggagtcg taacgcagaa
     3421 ccatgaaata atgcagtcga tattcatcga gatccaagaa tgaggtgatg tgtgaatggt
     3481 gattggatgg tatggatggg tgtagtggtg gccggagccg gaattggcta tgtcttgggc
     3541 gccagaagca ggcagatgcg aatccaccgg ctggaacagc aactgcagga aggcattcag
     3601 gctcagcgcc gttggttaga gaactggaag gccttttgcc agtgtctggc accggtgttt
     3661 ccggtatttg tgggacagat caaggcggtc attcaggaaa cagggcaagc ggccgacgga
     3721 ttgattcagc ggtttcaggc aatttcacga aatgccagag aacaggtgga ggaaaccgaa
     3781 gcgttgttac atatggcaga tgggcatggg gggaaagatg actataccgt tgatcggatc
     3841 cttcatgata cgcagaagac cattgaaatg ttcgtcaaac aagtcacaca gaccacgacg
     3901 gtgacgctcg ccacggtgag cgtgatggag caggccttag aaaccacctc tcgcatttca
     3961 gaggtggtgg aggaagtgga attcattgcc gatcaaacac gactcttagc gcttaatgcg
     4021 gcgattgaag ccgctcgggc gggagagcat ggccgggggt ttgccgtggt ggcggacgaa
     4081 gtgacgaaat tggccaatcg gtccgcgcaa gcctcagagc aaattcgtac tctcgcgacg
     4141 acggtgaaag gcacaaccga atcggccatg tcggaactcc aggtgttggc gtcattagac
     4201 ttatcggaaa cggtagaagc tcagaaccag gttctggaga tgatcaaaat tatgggtcaa
     4261 aaaaatgagg ccttaaaaaa gagcgtgggt cataattcgg ggcgaaccaa agaactgggc
     4321 aaggatatcg gacaaattgt gatgtccatg caattccagg atatcacccg tcaaaaatta
     4381 gagcatgtct accagccatt ggagcgaatc catgcgccgc ttcaagcggt ggccgacgat
     4441 actgacggtt tagacatgat gccgaaggtg ctcgaagaaa tccggaatct cgaacacacc
     4501 tacaccatgg agtctgaacg actcacgatg caggctgcgc gctcgggtca agggacggtg
     4561 atggtcggaa ccggctcgga tgaaggagat aacatcacgt tattctgaag atgtgagggg
     4621 tgaagggtca ggcgggaagg tgtaattttt ctccttacgt ctcaccgtta cggctttatg
     4681 aaaaaccggg ctggaggtcg caagttccat gctggtcgtg cagcgtcaac agagaaattc
     4741 acagaaccaa agtgaggtaa agctatgggc aagacagttt tagtggtgga cgattccgtc
     4801 tcgatgcgtc aaatggtgag ctttacgctg accggtgccg gctacgaagt ggtcgaggct
     4861 ggagacggaa aagaagccgt ggataaactc aatggagggg cgaagcccaa tttggtgatt
     4921 acggatctga atatgccgaa catggatggg atttctctga ttaaggcgat acggggcatg
     4981 gctgcccata agttcacccc gattctcatg ttaaccaccg agtcatctga tgacaaaaag
     5041 aaagaagggc aaagtgctgg agcgacgggg tgggttgtga aaccgtttaa tccagaacaa
     5101 atgttagcca cgattaagaa agtcctgccc ggctgagagg acgtgaggcg tgaaacgtga
     5161 ggcgtcaggc gtcaggggat aaggcgtgac acgggaggcg aaagctaaag ggaaatttga
     5221 ataagccaca tgaaaaattg gaatttagca atgcaacttg tgacggatgt gtaccaaata
     5281 acaaaaattt ttcctgaaca ggaaagatat ggtcttacca gtcagatgcg gcgagcggca
     5341 gtcagtattc ctagtaatat cgcggaaggt gcggcgcggc aaaccccaaa agagtttatt
     5401 cagtatttgc atgtttctca gggatcgtta agtgagctgg atactcaact ggatattagc
     5461 aaacgacttg ggtggttaca ggcaaaggat gtggatgaac ttgaaagtgc gatgaaccga
     5521 ctagataaga tgctgagtgg gttaatcagg cataaaaatc gtcacacctc ttacgcttca
     5581 cgcctcacga aaagataaat tctccgtcca ttcttaaagg agtcgaataa tggcagttga
     5641 tctctctcga tttcaagaat cattttttac tgaatccgca gaacatgtag aaaccatcga
     5701 gtccggttta ttggcgttag agcaacgtcc gaacgacctg gacttattga accgaatttt
     5761 tcgtggcgcc cattcgatta aaggcaatgc ggggatgttt cattttacgg ccattgccga
     5821 gttgactcac aaaatggaaa acatactgga tgacctccgg aatgagaaaa tgccggtgac
     5881 ccctcacgtc atcgatgtac tgttacgggc gttggatggt ttgaaaagtc ttttggacgc
     5941 ggcgcaaggg ggtgaggcgg ccaatgagac ggccattcac tccttagaag gtgaactgga
     6001 ggcctgtcag aaaggagcga ccgctcccgc acatgagggg tcttttgctg aagccggtcc
     6061 ccagggttct ccacctcaag gcgccacgtg gagaaagatc cagattgatt ggagtgcctt
     6121 gccggaatta tttcaacgag gcatggatcc cgcccagatt ttcaaggaac tgcatgagtt
     6181 gggcatggtg aaagtgctgc atgtgcgaac cgatggtctc ccggctttgg aggcactcga
     6241 tccagaacga tgttatctca gttggagggt ggaattggaa acagatgcct ctattagcca
     6301 gatcgaggag gtgtttgcgt ttgtccggga tgggagcgaa ttagccatca tcgattgcgc
     6361 accgaaaaag ccggaaccct ataaacgggt gggggatatt ctggtggaag aaggggtggt
     6421 gactccggag caaatcgccg aaagtctggc caagcaaaag cctcttggcg aaattctggt
     6481 ggaagagaaa aaggcgtctc cccaacaagt cgataaagcg cttcagaagc agctgcagtt
     6541 gaaaaaaaat gaagtggcgt ctattcgggt cgatacggaa aaaatcgata aattgatcaa
     6601 tctggtggga gaattggtaa ttacccaatc gatgattacg gacctgagtg agaagtttac
     6661 cctcgcgcag ctcccggttc ttcaggaacg gattacccaa ttggaacgca atacccggga
     6721 gatccaggaa cgggtcatgt ccattcggat gatgccgatc ggcagtgcat tccaccggtt
     6781 cccacggtta gttcgggatc tcgcagggaa aagtggtaag cagattcaat tggtcatgtc
     6841 cggtgaggaa accgagttgg ataaaaccct gattgaggcg attggcgatc cgcaggtcgg
     6901 tgtcgatgcc ggcgagccgg ctcgatccat cgaggaactg ccgctcggtg aagatgcccc
     6961 cgacgtgcga ctcggcgtag aggtcgtacc gcacgcggcc gatgaacgtg cgggccgatt
     7021 ccgcgaactc gagctcggcg tcatcgtcgg tctcgacggt ggccgcctcg tcgttcg
//
LOCUS       DATIAL010000180         6277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1431399, whole genome shotgun sequence.
ACCESSION   DATIAL010000180 DATIAL010000000
VERSION     DATIAL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431399"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..338
                     /locus_tag="VLA60_05610"
     CDS             102..338
                     /locus_tag="VLA60_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSF08869.1"
                     /translation="MAISAFVLIDTTGTHTKSAYKTLTRIQGVKSVYPVTGPFDLIAH
                     VEAETLEELNDLIMVRLRGIDGVIKTNTAIVLDF"
     gene            complement(366..1679)
                     /locus_tag="VLA60_05615"
     CDS             complement(366..1679)
                     /locus_tag="VLA60_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HSF08870.1"
                     /translation="MSHERLSTVVISGLTLTAILLVGIVIGKGGDRAGYASEPYEELQ
                     IFAEVLSQVKKNYVEETQTKDLVQGALRGMLAGLDPHSSFMTPDMFKEMQVETKGEFG
                     GLGIQIGVKNNRLTVISPIEDTPAFEAGIQPGDTIIKVDDKPTKDLTLMEAVQHMRGA
                     RGTSVELTIEREGLEKPLIFTLKRDIIKIQSVRSKLLEGNLGYVRLSQFQEATVDDLS
                     AELEKLASKNIQGLILDLRNNPGGLLTAAVGVSEQFLESGRLVVSIQGRNGKKDEYRA
                     RASSKNYQFPMIVLVNHGSASASEIVAAAMQDWGKAVVIGTTTFGKGSVQTILPLSDG
                     SGLRLTTAKYFTPNGKSIHSIGVQPDIVIDPKPVQVAKQESSEGKKDESTVGSPVPSP
                     AKPPVPSDPKDKEQPQSIPPEDLQLLKAVEMLKSWKVFKQLKPAA"
     gene            complement(1707..2903)
                     /locus_tag="VLA60_05620"
     CDS             complement(1707..2903)
                     /locus_tag="VLA60_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HSF08871.1"
                     /translation="MTRINLFFHTGVLLIGLLLVLVQAGWSADPSTIKDEISQERLRL
                     EKLNQEIAETQKETKRIEKKHGSVLKTIDKLDRQLYEQKKERNRINDQIKVTDEELAN
                     LSKKIGEVEQDVQTRQSSISARLRLLYMEGRTGYLKTLFAAESFTEAAHRMDYLSWVA
                     QREHQLVRQFQEDLSNLQQLKEKQTAAREDLVRFQGETQQTIKKVSGLKRQKRSVLVS
                     LSKEKDAHERMVEDLQRSAEQVDSLLKELDRRFQLAQSRMRQPPGALPSLGSLLWPVE
                     GKVVSNFGRQKHPTFDTFVTKKGIEISAQEGSPIRAVSSGNVVYADWLKGYGLVVIVD
                     HTNGFYSLYAHASKLLVAEGQSVKNGQVIGETGETGVTQEDTLYFELRKGTTPIDPLK
                     WLVKQP"
     gene            complement(2900..3799)
                     /locus_tag="VLA60_05625"
     CDS             complement(2900..3799)
                     /locus_tag="VLA60_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HSF08872.1"
                     /translation="MKRLWYFIREAYTSLRTHQTSTLIGILTTAFTLTSFGIFLLLYH
                     NVHNLLGQVQHTIQIIVYPKDDIESGKLRDLQQSLKADPVVDSLTFISKQDALTEFKE
                     QFPEEVYLLEGLGESPFPASLVLTMAAKTPTTDMIANLVNRLKHHPDIDRVRYNRDWI
                     ERLTLIIAYMEFGALVVGSILAIASVTIIAITVQLAFHTRQQEIEILRLIGATSLFIS
                     IPYLIEGAILGAAGGGMALGFLRGGFELFTHKTQGMSVFGGFSSALEFFPLPLSLILI
                     AGGILLGCLGTLTTIYGSLRFRL"
     gene            complement(3796..4464)
                     /gene="ftsE"
                     /locus_tag="VLA60_05630"
     CDS             complement(3796..4464)
                     /gene="ftsE"
                     /locus_tag="VLA60_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HSF08873.1"
                     /translation="MIQLFHVTKYYDGCLALSDISLRIEKGECVLLTGPSGAGKTTLL
                     KLIFGAEHPDEGQILVQNRNIARLGGSDVPYHRRTMGYVLQDFKLLPQRTVSENVALP
                     LIIRGLSSFESRRKVLEALRAVGMDHKLSTIASVLSAGEQQRVCIARAIVNSPIILLA
                     DEPTGNLDPQLTTEIAALFKAINTRGTTVMMATHNRQVVEQLNGRILRLDCGHLVSDQ
                     GAYT"
     gene            complement(4604..4990)
                     /locus_tag="VLA60_05635"
     CDS             complement(4604..4990)
                     /locus_tag="VLA60_05635"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HSF08874.1"
                     /translation="MTTPSHQFGRSAEEFAEEWLRKKGYRILERNLRVGGGELDLIAQ
                     HHDTLVFIEVKGRRTNQFGGAPYAIDSRKKRQIIKLASHYLSQQGLSNQVCRFDVILI
                     AGTTQGTPKLTHLEQAFEVSSSDWQW"
     gene            complement(4987..5640)
                     /locus_tag="VLA60_05640"
     CDS             complement(4987..5640)
                     /locus_tag="VLA60_05640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HSF08875.1"
                     /translation="MQTDSPSLMGTGPTDFFEQAARALGYRRIAGLDEAGRGPLAGPV
                     VAAIVILPRRWSPVLLDDSKLLTEQQRQVLYEAITTRAIAWAIGMASEQEIDELNILG
                     ATRLAGCRALSYLATPPDYLLLDALHLPRVSVPQRPVIKGDQLSRSIAAASILAKVSR
                     DRMMTTYHERFPQYQFHLHKGYPTPEHLRRLQQFGPCPGHRRSFRPVQACLNVPSLA"
     gene            complement(5666..6124)
                     /gene="rplS"
                     /locus_tag="VLA60_05645"
     CDS             complement(5666..6124)
                     /gene="rplS"
                     /locus_tag="VLA60_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HSF08876.1"
                     /translation="MNLLERLEQSLAQGTVPFFEIGDTVRVKVRVVEERQYGKKKEEA
                     KERIQVFEGLVIARKGRKVCENFTVRKISFGVGVERIFPLHSPNLAGIEVIRKGQVRR
                     AKLYYLRDKKGKAAKISEREFSRTPKTVKNAAAAVEVPESGESEPTEIAE"
BASE COUNT         1466 a         1552 c         1603 g         1656 t
ORIGIN      
        1 tcttctccca tggtttccga cggacgcaat ccccatcgcg atggaacagg aaaagggtat
       61 ggaaacatat caggcattca cctcagcagg atggttaggt aatggcaatt tcggcatttg
      121 ttttaattga cacgacaggc actcacacca agagtgcata taaaacactt acacgcattc
      181 aaggggtgaa atcggtctat ccggtgacag gtccgtttga cttaattgcc catgtggaag
      241 ccgaaacttt agaggagctg aacgacctca ttatggttcg tcttcgaggt attgatggcg
      301 tcattaaaac gaatacggca attgttctcg acttctaaat tcttacacga cttatgccgg
      361 ccgcgttatg cggccggttt taactgctta aagactttcc aggatttcaa catttctaca
      421 gccttgagta attgaaggtc ctcgggcgga atggactggg gctgctcctt gtccttggga
      481 tccgagggga ctggaggttt ggcaggagaa ggtacagggg aacccacagt actctcatcc
      541 tttttccctt cggaggattc ctgttttgcc acctgaacgg gtttcggatc aatgacaatg
      601 tcgggttgca ccccaataga atgaatcgac ttgccgtttg gcgtaaagta tttcgccgtg
      661 gttaggcgaa ggcctgaccc atcggagaga gggagaatgg tttgcaccga cccctttcca
      721 aaggttgtgg tgccaataac gacggctttc ccccaatcct gcatggctgc agccacgatt
      781 tctgatgcgc tggcagaacc gtggttgacc aacacaatca taggaaattg atagttctta
      841 ctacttgccc gtgcgcgata ttcatccttt ttgccgttgc gtccttgtat cgagaccacg
      901 agccggccgg attccaggaa ttgttcggag actcctacgg cagccgtcaa cagcccaccg
      961 ggattatttc gaagatcgag gattaaaccc tgaatgtttt tggaagctaa tttttccaat
     1021 tccgcggaga gatcgtcgac tgtcgcctct tgaaattggc tcaaacgaac gtatccgagg
     1081 ttgccttcca gcagcttgga gcgaacactt tgaattttaa tgatatcgcg tttgagggta
     1141 aagatcagcg gtttttccag cccctcacgc tctatggtca attccacaga agttccccgt
     1201 gccccgcgca tgtgctgaac ggcctccatg agcgtgagat cttttgttgg tttatcgtca
     1261 actttgataa tggtgtcacc gggctggatg ccggcttcaa aggccggcgt atcctcaata
     1321 ggagagatca cggtcaggcg attgtttttt acgccgattt gaatgccaag tcctccgaac
     1381 tctcccttcg tttcaacttg catttctttg aacatatcag gagtcataaa agaagaatgt
     1441 ggatcgagtc cggccagcat gcctcgtagg gcaccctgga caagatcttt ggtttgcgtt
     1501 tcctccacat aattcttttt gacttgcgaa agcacttcag cgaagatttg caattcctcg
     1561 tagggttcgc tggcataccc cgcccggtct cctccttttc cgatgacaat ccccaccaaa
     1621 aggatggcgg ttaaggtcaa accagaaatc actaccgtcg acaatcgttc atgtgacatg
     1681 aggtgttcct tcttccagtt gttcaattaa ggctgtttta ccagccattt caggggatca
     1741 atgggggtgg tgcctttacg taattcaaaa taaagggtgt cttcttgggt gacgcctgtt
     1801 tcgcctgttt caccaatgac ctgcccgttt ttgacagatt ggccttcagc cactaagagc
     1861 tttgacgcat gggcatagag agaataaaag ccattcgtat ggtcaacgat gacgaccagg
     1921 ccgtatccct tgagccagtc ggcatagacg acgtttcccg aggaaacggc gcgaatcgga
     1981 ctcccttcct gtgcactgat ttctatgccc ttttttgtca caaatgtatc aaaagtggga
     2041 tgtttttgcc gaccaaaatt cgaaaccacc ttcccttcca ccggccagag gagggatccc
     2101 agagatggga gagctccagg cggttggcgc atccgggatt gagccaattg aaaacgtcgg
     2161 tccaattctt tgagaaggct atccacctgc tccgctgaac gctgaaggtc ttcaaccatc
     2221 cgttcatgtg cgtctttttc cttggagaga ctgaccagaa cggagcgttt ttgacgtttg
     2281 agccctgaaa cttttttgat ggtctgttgt gtttctccct gaaagcgaac aagatcctcg
     2341 cgggccgccg tctgtttctc cttgagctgt tggagattcg aaaggtcttc ttgaaactgt
     2401 cggacaagct gatgctcccg ttgggcaacc catgaaagat aatccatccg atgtgcggcc
     2461 tcggtgaatg attcagctgc gaagagagtt ttcaaatagc ctgtgcgtcc ctccatgtat
     2521 aataaccgaa gtctggcgga aatggaggat tgccgggttt gaacgtcttg ctcaacttcc
     2581 ccaatcttct tgctcaggtt cgccaattcc tcgtctgtta ctttgatctg gtcatttatg
     2641 cgattgcgtt ctttcttttg ctcatagagc tggcggtcca atttatcaat tgttttcaaa
     2701 accgacccat gctttttctc gatccgtttg gtttcttttt gggtttcggc aatttcctga
     2761 ttgagctttt ccagccgcag gcgttcctga ctgatttcat ccttgatggt tgatgggtca
     2821 gcgctccaac cggcttggac taagaccagg agcagtccga ttaggagcac gcctgtgtgg
     2881 aaaaataggt taattcgcgt catagacgaa atcgcaacga tccataaatg gtagtcagtg
     2941 ttcccaggca ccccaataga atgcctccgg ctatcaaaat gagtgaaagg ggaaggggga
     3001 aaaactctaa tgcggaggaa aatcccccga acacgctcat gccttgtgtt ttgtgcgtaa
     3061 aaagttcaaa ccccccacgt aagaatccta aggccatgcc gcccccggca gcccctagga
     3121 tagccccctc gatgagatac ggaatgctaa taaagagact cgtcgccccg attaaccgta
     3181 aaatttcaat ttcctgctgt ctggtatgaa atgccagttg cacggtaatc gcaatgatgg
     3241 tgaccgaggc tatggccaag atgctcccaa cgactaacgc gccgaattcc atataggcga
     3301 taatgagggt taggcgctcg atccaatctc gattataccg aacccgatcg atatcgggat
     3361 ggtgtttgag ccgattgacc aggttggcaa tcatatcggt ggttggagtc ttggcggcca
     3421 tggtcaatac taatgatgcg ggaaacgggc tctcgccaag accttccaaa agatagactt
     3481 cctcgggaaa ttgttctttg aattccgtta aggcgtcttg ctttgaaatg aaggtgaggg
     3541 agtccaccac tggatctgcc ttgaggctct gttgtaagtc acgaagtttg ccagattcaa
     3601 tgtcatcctt gggatagacg atgatttgaa tcgtgtgttg aacctgaccc aacagattat
     3661 gcacgttatg atataaaagg agaaagatgc caaagctggt cagagtgaac gcggtggtga
     3721 gaatgccaat cagtgtactg gtctgatgcg tgcggaggct ggtataggct tcacggataa
     3781 aataccatag ccgtttcacg tatacgctcc ttgatcagac acgagatgtc cgcaatccag
     3841 tctgaggatt ctgccattca attgctccac aacttgtcga ttgtgggtcg ccatcatgac
     3901 ggtggttccc cgggtattga tggctttaaa taatgccgca atttcggtgg tcagttgggg
     3961 gtctaaattt cccgttggtt cgtcggctaa cagtatgatg ggactgttca caatcgctct
     4021 ggcgatacac acgcgttgct gctctccagc ggacagcacg gaggcaatgg tgctgagttt
     4081 atggtccatg ccaaccgcgc gaagggcttc caggaccttc cgccgcgatt cgaacgatga
     4141 caacccacga ataatgagtg ggagggccac attttcggag accgtccgct gtgggagcag
     4201 cttgaaatcc tgaagaacat accccatggt tcttcgatga tagggaacat cggatcctcc
     4261 gagtctggcg atgttccgat tttggacgag gatttgacct tcatcagggt gttctgctcc
     4321 aaaaataagc ttgagtaggg tggttttgcc ggctccgctt ggtcctgtga gtaacacgca
     4381 ttcgcctttt tcgattcgta gggaaatatc tgaaagcgca aggcagccat cgtagtattt
     4441 ggtgacgtga aataattgaa tcatgctatc ttatggcctg gaccctgaaa tggcttatta
     4501 tctagataaa acggggccat ggttcctggg tgcgggtata cagaaagctg ccgaccgttc
     4561 acctgcaggc attggacctc gtgcagaagc caacctcttt aatttaccat tgccagtcgg
     4621 agctggaaac ctcaaaagcc tgttccaggt gagttaattt cggagtaccc tgggtggttc
     4681 ccgcgatcag gataacatca aaccggcaaa cttggttgct gaggccctgc tgagaaaggt
     4741 aatgcgacgc caatttaatg atttggcgtt ttttgcgcga atcgatggca tagggagcgc
     4801 cgccgaattg attagtgcgt cgtcccttga cctcaataaa caccagggta tcatggtgtt
     4861 gtgcaatgag atccaattct ccacccccta ctcgcagatt tcgctcaagg atccgatagc
     4921 ctttttttcg gagccattcc tctgcaaact cctccgctga tcgcccaaac tggtgagaag
     4981 gcgtggtcat gcgagcgagg gcacattcag gcaggcttgg acaggccgga agcttcgtcg
     5041 atgcccagga cacgggccga actgctgtag gcgtctgaga tgttcgggtg tgggatatcc
     5101 tttgtgtagg tgaaattgat actgaggaaa acgttcgtga taggtggtca tcatgcgatc
     5161 ccgtgatact ttggccagaa tagatgcggc ggcaatagac cgtgaaagtt gatcgccttt
     5221 gataaccggt cgttgaggga cagacactct agggaggtgc aaagcatcca gaaggaggta
     5281 atctggtgga gtggccaggt agctaagagc ccggcaaccg gccaatcgtg tggcccccag
     5341 gatgttgagt tcgtcaatct cctgttctga tgccatgcca atggcccaag caatggcccg
     5401 ggtggtaatg gcctcatata atacctggcg ttgctgttca gtcaggagtt tggaatcgtc
     5461 gagaaggacc ggtgaccaac gacgtggaag aatcacgatc gcggcgacca ccggcccggc
     5521 caaaggaccg cgcccagcct catccagtcc tgcaatgcgc cggtatccaa gggctcgggc
     5581 cgcttgttca aaaaaatctg tcggacccgt acccatcaat gagggagagt cggtttgcaa
     5641 tgaaaggtga gccctttctc ctgcgttatt ccgctatttc cgtgggttcc gattcaccgg
     5701 actcgggaac ctcgactgcc gccgcagcat ttttcaccgt ttttggtgta cgcgaaaact
     5761 cccgttctga aattttagcg gctttgcctt ttttgtctcg aaggtaatac agcttcgccc
     5821 gccgcacttg accctttctg atgacttcga tgccggccag attaggagag tgaaggggaa
     5881 aaattcgctc gactccgact ccaaaggaaa tcttacgaac ggtaaaattc tcacatacct
     5941 tgcggccttt tcgagcgatc acgagtcctt caaacacctg aatgcgttct ttagcctctt
     6001 ccttcttttt cccgtattgt cgttcttcga cgacccgcac tttgactctg acggtgtcac
     6061 ctatttcaaa aaacggcacg gttccctgtg ccaacgattg ctctaaacgc tccaacagat
     6121 tcatgagagt ctccctcctt caaggtctcg tcgtacgaac ggacgtcgag gtatagttgt
     6181 gttcaatctc ttccaataac tcacgatctt cactactcca agtatgatga tttaacaaat
     6241 ccggccgttt cagcagcgtc tggcgtaagg attcttt
//
LOCUS       DATIAL010000181         7366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1431416, whole genome shotgun sequence.
ACCESSION   DATIAL010000181 DATIAL010000000
VERSION     DATIAL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431416"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..712
                     /locus_tag="VLA60_05650"
     CDS             251..712
                     /locus_tag="VLA60_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSF08877.1"
                     /translation="MVIRRLENWPFDGISGGVSELDRLRRDLGHLFNGLSRVNGWDSP
                     AGVYPLMNVSQDSENVYVRSEVPGMTLDQLEVSVTGRNLTVTGERAIPDEQSNVRYHR
                     REREAGKIRRQLTLPTDVDSERVQAKYQHGILMVVLPKAEKAKPKKIAITG"
     gene            773..1180
                     /locus_tag="VLA60_05655"
     CDS             773..1180
                     /locus_tag="VLA60_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSF08878.1"
                     /translation="MTTQTQDIQVRDKREVKSDAEQTIPGRVFSPNVDIFEDDQALTI
                     VADMPGVPSENVSIDLRNDVLSLSGVPSVSVPEKEEHVLREYETGKYFRQFTLSEVID
                     QEHIEAKLNNGVLRVTLPKVGPAKPRKIQITEG"
     gene            complement(1402..1677)
                     /locus_tag="VLA60_05660"
     CDS             complement(1402..1677)
                     /locus_tag="VLA60_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HSF08879.1"
                     /translation="MVTITLMGDLQTVDGERALGCEIADSVTVKQLIRKQGKPLREIF
                     QLLKEKKVMVTVNKRIASEETKVYDGDAVYIVAHDGMGQSGLTLSHY"
     gene            complement(1766..2488)
                     /locus_tag="VLA60_05665"
     CDS             complement(1766..2488)
                     /locus_tag="VLA60_05665"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSF08880.1"
                     /translation="MSQPLTGTIVDLTYPFDEHTVYWPTNESFHWKKTNWGPSKQGYW
                     YASAVYSASEHGGTHLDAPIHFAESGLSVDEIPVDQLTGDAILLDIRAQVESNPDYTL
                     RVDDIAQWEISYGPIPPKAIVLLFTGFGQYWPDKARYLGSSTPEDPTTLHFPGFSAKA
                     ITFLLTKRDILGIGIDTASIDAGQSRDFPVHQILGRANRYALENVAHLELLPPRGARM
                     TALPMKIQGGTGGPVRIIATVP"
     gene            2739..2987
                     /locus_tag="VLA60_05670"
     CDS             2739..2987
                     /locus_tag="VLA60_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HSF08881.1"
                     /translation="MYKIITILILLVILFFMVRRAFREWSQPKLDPATPGKDVMIQDP
                     VCKVYVAAGTAIVQELKGKNYYFCSQDCARHFKPEDVT"
     gene            complement(3033..4043)
                     /gene="arsS"
                     /locus_tag="VLA60_05675"
     CDS             complement(3033..4043)
                     /gene="arsS"
                     /locus_tag="VLA60_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HSF08882.1"
                     /translation="MKTSALPMVAPDQSSGMVPSFEQALAPHHSLPLKAQSISTLQVN
                     VGKVCNQTCHHCHVDAGPQRTESMSKATMDQILDVLDRTPQIMTVDITGGAPEMNPNF
                     EYLVEQCRVRNRKVIDRCNLTVFYVKGKAHLPQFLADHHVDIVASLPCYEADNVDQQR
                     GKGVFNRSITALQTLNDLGYGTDGTGLMLDLVYNPLGPVLPPPQVELEQDFKEELGQR
                     YGIQFNRLYTITNMPISRFWDELRDAGQLEHYYTLLLNNFNPLAIEGLMCRSLLSVGW
                     DGRLYDCDFNQMLDLPVQPESQAWIDRFNLMELEHRPIVVGPHCLGCTAGSGSSCGGA
                     LT"
     gene            complement(4098..5303)
                     /locus_tag="VLA60_05680"
     CDS             complement(4098..5303)
                     /locus_tag="VLA60_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF08883.1"
                     /translation="MANETKEENHSITQAVSQRYAKAVTNGEQLCCPTGYNHEDLGQF
                     IPEPVLKVSYGCGTPVGLSTVQPGEVVLDIGSGGGIDCFEASRKVGPRGRVIGIDMTD
                     EMLALARAHAPTVASNLGYPEPNVDFRKGFADAMPVEDDQVDLIISNCVINLAPDKRK
                     VFQEMFRVLRPGGRFTISDIVADQPIPNYLIYDKAKWGDCLSGALTIKDYWGGLRDAG
                     FKGLHQVNSIPWRVIDGIQFLSVTLTGYKLASTSTTPTSAFATLTGPFSQVLDELGTT
                     FTRGHPQQIDERTAELLALAPYHERFIIDEKPVALAVQDSRMLAVYPEDAPCLWEGQF
                     AVLTGPFLEVCDDDHHTYQCGEPVEICSKTHKVLQTTSYQPYFAIINRSREGVDSEPV
                     ICGPSTGCC"
     gene            5705..6331
                     /locus_tag="VLA60_05685"
     CDS             5705..6331
                     /locus_tag="VLA60_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSF08884.1"
                     /translation="MALVPCPECHKEVSTEARACPQCAFPSPGKHSLEEERASGLHTC
                     PQCHGLVSPDARTCPYCRMSLAGGLPQQEDNGESIQETLVCPHCRASYIHTRKIPQPV
                     KPETTSPGKIPSIAPVKRVATKGSRASQVEPLRKTTALLGPRRPSALWQDPSGSQKVP
                     PPRYPRSKKNSILIGLILLVIVTISVVFGAMWQLNGLNPLETILSWRM"
     gene            complement(6490..>7366)
                     /locus_tag="VLA60_05690"
     CDS             complement(6490..>7366)
                     /locus_tag="VLA60_05690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSF08885.1"
                     /translation="GLAIGYILIFAALGFKIGAVPFHAWIPDTYQGAPTPVTAFLSIA
                     PKAAALAILIRMFYVSLASFKPMWVLLFVVASIFSMTYGNIVAIAQKNIKRLLAYSGI
                     AQIGNIMIGLAAGTKQGSDAIMFFLLTYLFANLGAFAVIIAVSRVMKSDEIEDYNGLN
                     RRSPFLAASMLLFLLSLAGVPPLAGFIGKIYLFIAAMKEELYTLLIVGLINIVISMYY
                     YLIVVKKMYINEPTDSSPILASMPIKVAVYVGIAGTVLLGVYPGPFIDWAVSASLMFS
                     NIAGSTAAASLLPGG"
BASE COUNT         2013 a         1831 c         1774 g         1748 t
ORIGIN      
        1 cgctggttgg gcgccctgtg tttcttatta tttccgcttt cattctcgtt ccttcctcga
       61 tctgttgtac cgaacaatcc cactcctgtt aagcatttcc cccgaaaatt atcgtcctac
      121 ttgactcccg gaattttgaa attattttat aggtaacatc gatgcatgtt tgaccttcga
      181 aggtatcccg atgaattaac aagcatttgg aagaatggag tacgagaagt tcaaagagga
      241 ggggtaaact atggtcattc gaagattaga aaattggccg tttgatggta tttctggggg
      301 agtttcagag ctggaccggc ttcgacgaga tctcgggcac ttgttcaatg gactatccag
      361 ggtgaatggt tgggattctc cagcgggcgt ctatccgctt atgaatgtgt cacaggatag
      421 cgaaaatgtt tatgttcgct cggaagttcc aggcatgacg ctcgatcaac tggaggtgtc
      481 agtcacaggc cggaatctta ccgtcacagg tgagcgggcg attccggatg agcaatccaa
      541 tgtgcgatat catcgtcgag aacgggaagc cggaaaaatc cggcgacagt taacattacc
      601 cacagatgtg gacagtgagc gagtgcaagc aaaatatcaa cacggtattt taatggtggt
      661 cttgcctaaa gccgaaaagg ctaaaccgaa aaaaatcgcc attacaggat aaagattgac
      721 atagaggtga gcgatgggga agaccccatt caatacaaag gaggatgaac acatgacaac
      781 acagacgcag gacatacaag taagggataa gcgggaagtc aaatcggatg cggaacaaac
      841 gatccctggg cgggtgtttt cacccaatgt cgacatattt gaagatgatc aggccctgac
      901 aattgtggcg gatatgcccg gggtgccatc cgaaaatgtc tcaattgatc ttcgcaacga
      961 tgtgctctcg ttaagcggtg tgccgtcagt ttctgtgcct gagaaagagg agcatgtttt
     1021 acgcgagtac gagaccggga aatatttccg acaatttact ctttctgagg tgattgatca
     1081 ggaacatatt gaagccaaac tgaataatgg agtattgcga gtaactctgc caaaggtggg
     1141 gcctgccaaa cctcgcaaga ttcaaatcac tgaaggataa tcagatctca acattcgagg
     1201 gagccgttcg aataaagcgg ctcccttttt tttttgcatg ggtgatcgct caccgggaat
     1261 ggacgatttg gagccctttg atacgctgac aggagatccg catcaggaca gaagtctcta
     1321 caagaagact aagactgaag cccatgggtg aaccatccga gaacccgatt ggcccgggcc
     1381 catcaacatg gccaacttga attagtaatg ggagagcgta agtccgcttt gtcccattcc
     1441 atcgtgagcg acaatataaa cggcatctcc atcgtagact ttagtttctt cactggcaat
     1501 tcgtttattt acggtcacca tgactttttt ttcttttaac aactgaaaga tttcccgaag
     1561 cggtttgcct tgtttcctga tcagttgctt aacggtgacg gagtcagcaa tctcgcatcc
     1621 aagggctcgc tccccatcaa cagtttgcaa atcacccatt aacgtaatcg taaccataat
     1681 ttccttttgt tcatcatcac tatttctcca agtatgtttg agagggccca aagacctttc
     1741 attcagtgtt tgatctgatg agcccctaag gcacggttgc aataattcga accggtcctc
     1801 ctgttcctcc ttgaatcttc atagggaggg ccgtcattcg agctcctctg ggtggtaaga
     1861 gttcgagatg cgccacattc tccaacgcat agcgatttgc tcgacccaga atttgatgaa
     1921 ctggaaaatc tcgggactgc ccggcatcaa tgctggctgt atctattcca attcctaaaa
     1981 tatctctttt cgtaagcaga aatgtgatgg ccttggcaga aaaccccggg aaatggagcg
     2041 tagtgggatc ctccggagta gagctaccaa gatatcgggc cttgtccggc caatactgtc
     2101 caaacccggt aaagagtaac acgatggctt tgggagggat tggaccgtac gagatttccc
     2161 attgggcaat atcgtcaacc cgtagagtat aatccggatt ggactccacc tgcgctcgaa
     2221 tatccaataa aatcgcgtca cctgttaact gatcgacggg gatttcatcc acactcaatc
     2281 cggactctgc aaaatgaata ggggcatcga gatgggtgcc accatgctct gaagccgagt
     2341 agactgccga tgcataccaa tacccctgct tgctggggcc ccaattcgtt ttcttccagt
     2401 gaaaggactc attcgttggc caatacacgg tatgctcatc aaagggatag gtcagatcga
     2461 cgattgttcc cgtaaggggt tggctcatgg tgcatccccc gaatgcgaga accattagca
     2521 agacccggat caacatgggc tgagacctac gaggttcaga ataaagacca acggcagcat
     2581 agggaaaatc ctaacaagtt gagaaagggc acagcaatgg aaataaagcc atgtgaatca
     2641 ccaatcatct tgggaaaggg ccgtgtacgg catatgtgga atcaaacccg aaaacatggt
     2701 acaaaatgct atctatctca aaaggctcat cacacaccat gtataaaata attacaattc
     2761 tcattctttt agtgatcttg ttctttatgg ttcgtcgagc tttccgtgaa tggagtcagc
     2821 ccaaacttga tcccgccacc ccagggaaag atgtgatgat tcaagatccc gtctgtaaag
     2881 tatacgtagc tgcgggcaca gcgattgtgc aggagctaaa aggaaagaat tattatttct
     2941 gcagccagga ttgcgccaga catttcaagc ctgaggacgt cacctaaaga cccgattcga
     3001 aacctgatga ttgatgatgg aaggcaggcg aattaggtca gggctccacc acacgatgaa
     3061 ccggaacctg ccgtgcaccc aaggcaatgg ggcccgacca caattggacg gtgttccagt
     3121 tccatcaaat tgaaccggtc tatccaggct tgagattccg gctggaccgg cagatccaac
     3181 atttgattga aatcacagtc atagaggcgc ccgtcccatc ccacactcag cagagaccga
     3241 cacatcagcc cctcaatagc caatggattg aaattattta ataagagcgt gtagtaatgc
     3301 tctagttgtc ctgcgtcgcg caactcatcc cagaaacgac tgatcggcat attggtgatg
     3361 gtgtaaaggc gattaaactg tatgccatat cgctgcccca attcttcttt aaaatcttgt
     3421 tctagttcga cttgaggcgg tggaaggaca gggcctagcg ggttatagac cagatccagc
     3481 atgaggccgg ttccgtctgt tccatacccc aagtcattga gagtctgcag cgcggtaatg
     3541 ctccgattaa acacgccttt tcctcgttgc tgatcgacat tgtcggcttc ataacagggg
     3601 agggaggcga cgatgtccac gtgatggtct gcaagaaatt gaggaagatg ggctttgccc
     3661 ttgacataga acaccgtcaa attacagcgg tcgatcacct ttcgatttcg tacccggcat
     3721 tgctcaacca gatactcaaa attcgggttc atctcaggcg cccctcccgt gatatccacc
     3781 gtcataatct gaggggtgcg gtcaagaacg tccaggatct gatccatggt ggctttgctc
     3841 atgctttccg tccgttgagg cccggcatcg acatggcaat ggtgacaggt ttggttacag
     3901 accttcccga cattgacctg aagcgtggaa atcgattgag ccttgagggg aagcgaatga
     3961 tggggtgcta acgcttgctc gaacgacgga accatcccgg aagattgatc cggggcaacc
     4021 atggggagag ctgaagtttt cattgttttg atggtcttaa ttcctcacac accgacaggg
     4081 tgattgttcg gaaattccta acaacacccc gttgatggtc cgcaaatgac gggttcggag
     4141 tccacgccct ctctggatcg attaatgatc gcgaaatagg gttgatatga agtggtctgt
     4201 aggaccttgt gtgttttaga acaaatttct accggctcac cgcactgata cgtatgatgg
     4261 tcatcgtcac aaacctctaa aaacggtccg gttaacacgg caaattgtcc ttcccataga
     4321 caaggcgcat cttcgggata gacagctagc attcgggaat cttgaaccgc cagagccacc
     4381 ggtttctcgt caataatgaa tcgctcatga taaggagcta atgccaataa ttcggccgtt
     4441 cgttcatcga tttgctgagg atggcctcga gtaaatgttg tacccagctc atccagcacc
     4501 tgagagaatg gcccggtcag ggtggcaaag gccgacgtcg gagtggtcga tgtggaggcc
     4561 agtttatacc ccgtgagagt cacagaaagg aactggatgc cgtcaatcac tctccatgga
     4621 atactattaa cctgatgaag ccctttgaat ccggcgtctc gaagcccgcc ccaatagtct
     4681 ttgatcgtaa gggcgcctga taaacaatcg ccccatttgg ccttgtcata tattaagtaa
     4741 ttaggaattg gttggtcggc aacaatatcg gaaatggtga accgtccacc aggtcggagc
     4801 acgcgaaaca tttcctggaa gaccttcctc ttatccgggg cgagattaat aacacaattc
     4861 gaaataatga gatccacctg atcgtcttcc acaggcatgg cgtcggcaaa tccctttcga
     4921 aagtccacat tcggctccgg ataacccaga ttgctggcaa ctgttggtgc atgcgcacgc
     4981 gccaacgcca acatttcatc ggtcatatca attccaatga cacgacctcg gggcccgact
     5041 ttccgagacg cttcaaaaca gtcgatcccg cctccagaac caatgtccag gacgacttcc
     5101 ccaggttgca cagttgaaag gccgaccgga gttccgcagc cataagacac cttcaagacc
     5161 ggttctggaa taaattgtcc caaatcctca tgattatacc cggtcgggca acacaactgt
     5221 tcaccgttgg tcaccgcttt agcgtatcgc tggctgaccg cctgggtgat agaatgattt
     5281 tcctcttttg tttcattggc catagtaccc tcatggaaat ttgaaaaacc ctgttaattc
     5341 cttgcatcct gctatcataa taaggcatag aacaaggaat caccccaaga taacagtgtc
     5401 tggacatgcc gggaaatgtt acacatttta ctcctaaaag agaacataga gatgaaatca
     5461 aggaacccgg aacccaattg tgaaaaaatc cgctctccct tgagaagagc aaatcagatg
     5521 agagagaata ggtgaaggct aggccctccc cttcatcttc cattcggttc aagaaggagg
     5581 aagacactcc tgcatttaca cataaatcaa gcaaattttc caggtatcga ctttgccttc
     5641 ctgaaagatc aggaaaaaat atgctcgaaa aatctgtgaa atagcgagaa cggaaattta
     5701 ctccatggcc cttgttccct gccctgaatg tcacaaagag gtctccacag aagctcgagc
     5761 ctgtcctcaa tgcgccttcc cttctcccgg gaagcactca ttagaagagg aacgtgcctc
     5821 cggactccat acctgtccac aatgtcacgg tctggtctcg ccggatgccc gcacatgccc
     5881 ctattgccgg atgtcgttag caggaggact cccacagcag gaggataatg gcgaatcaat
     5941 ccaggaaacg ctggtgtgtc cgcattgtcg agcatcctat atccatacca ggaaaattcc
     6001 ccaacctgtg aagccagaga ccacgtctcc agggaaaata ccctcaatcg ccccagtgaa
     6061 aagggtagcc acaaaaggct cacgagccag tcaagtggaa cctctccgga aaaccacagc
     6121 cctcctgggg ccacgacgac cctctgcatt gtggcaagac ccgtcggggt cccaaaaagt
     6181 tcctcccccc cgttatcccc gtagcaaaaa aaattcgatc ctcatcggtc tgattctctt
     6241 agtgattgtg accatatccg tggtattcgg agctatgtgg caacttaatg ggctcaatcc
     6301 cctggaaacc atcctctcct ggcgaatgta actccaccaa atctcccttt tcctaatacc
     6361 ccatcaagct gtacccatcc ccagcccaca ttctcccctt tcaaggatgt cacgtggaat
     6421 cgaggaaaaa gagattctgc ctgtcaatcg gagaaggagt gtggaacgcg caaatatccg
     6481 gcccggcgat tatccgccag gaagtaggga cgcggcggcg gtagatcctg cgatattgga
     6541 gaacataagg gaggcactaa cggcccagtc gataaacggt cctgggtaca cacccaacaa
     6601 caccgttccg gcaatcccga catataccgc aactttgatc ggcattgatg caagaattgg
     6661 cgaagaatcg gtgggttcat tgatgtacat ctttttaacg acaattagat agtagtacat
     6721 ggagatgacg atattgatga gtccgacaat cagaagggtg tatagctcct ctttcatggc
     6781 cgcaataaat aaatagattt tcccgataaa tcccgcaagc ggaggtactc cagccagcga
     6841 caacaggaag agcaacatgg acgccgccag aaacggggaa cgtcgattca gaccattata
     6901 atcttcaatt tcgtcgcttt tcatgacccg actcacggca atgatgacgg cgaaagctcc
     6961 caaattcgca aaaagatacg tcagaagaaa aaacataatc gcatcgctgc cttgcttcgt
     7021 ccctgcggct aatccgatca tgatatttcc aatttgcgca atgccggaat aggccaaaag
     7081 ccgtttaata ttcttttggg caatggccac gatattgccg taggtcatgg agaagatgga
     7141 tgcgacgaca aataacaaca cccacattgg cttgaatgaa gcaagagaca cgtaaaacat
     7201 cctgataagg atagccaatg ctgcggcctt gggtgcaatg gagagaaagg ccgtcacagg
     7261 tgtcggtgcc ccttgatagg tgtcaggaat ccaggcgtga aaagggactg caccgatttt
     7321 gaatcccagg gcggcaaaga tcaaaatgta gccaatcgcc agtcca
//
LOCUS       DATIAL010000182         4617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1108384, whole genome shotgun sequence.
ACCESSION   DATIAL010000182 DATIAL010000000
VERSION     DATIAL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108384"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            786..1826
                     /gene="hflK"
                     /locus_tag="VLA60_05695"
     CDS             786..1826
                     /gene="hflK"
                     /locus_tag="VLA60_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HSF08886.1"
                     /translation="MAWEPKDPWGGSGQDPLDEALRKAQDQLTRLVPGRGFKSIIYIV
                     LAVLALWQSVFIVAPDEQGLVKRFGDIVREVESGPHFKIPFVETVDTPKVEKLHRVEV
                     GFRTDRGRTQFVPKEALMLTGDMNILSLEFIVQYKIKEAKNYLYKVADVESTIHNAAE
                     AAMREVIGKSKIDEALTIGKAQIQQEAQDLLQGVLDQYLAGVQVATIQLQDVNPPEAV
                     AAAFKDVASAKEDREKLINQSHGYRNDLIPRAKGEAAQGINQAKGSAQSRIARAEGEA
                     NYFLQTLKEYKLAKDVISKRVYIETMEEVMANTEKIILDSKAAGNVLPFLPLDRQSGS
                     RGATNTQGGDSR"
     gene            1823..2680
                     /gene="hflC"
                     /locus_tag="VLA60_05700"
     CDS             1823..2680
                     /gene="hflC"
                     /locus_tag="VLA60_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HSF08887.1"
                     /translation="MKQNLLIGVILVFGLLLVLGLSPFFVVDLTETAIVVALGKPVQT
                     ITEPGLKFKIPFYHQVTFFDKRYLDYDAPARDVITSDKKSLVIDNFAKWRIVDPLKVY
                     QAFQTQRGALQRLDDIIYSELRVELGRHELGEIVAANRALIMKVVSDRSNEKASAYGL
                     EVLDVRIKRADLPEQNEKAIFQRMQAERERQAKQYRAEGEEEAQKIRSEAEKDKQIIL
                     AEAYKTAQEIRGDGEAKAYKIYATAYQQGPEFFEFIRTMEAYKKTFANNTTLVLSPDS
                     EFLKYLKKR"
     gene            2747..3412
                     /locus_tag="VLA60_05705"
     CDS             2747..3412
                     /locus_tag="VLA60_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF08888.1"
                     /translation="MANPEHVERLIKGGVGEWNKFRRTNQTIQPDLSGADLSGYDFTG
                     LDLSQANLEGADLSDAYLFHANLTEANLSGVNLTEADVQEANLIKANLSHATMTRIDL
                     SGTGLVACNLEGANLEGATLTRASLPWGNLTRANLAKAKLHLTDLREAQLIQANLEGA
                     SLQDTSLTGTNLQGANLQGATHVSIDLLAKAKSLYQTILDPDLKGTLLQCHSHLFEKP
                     VDL"
     gene            complement(3640..>4617)
                     /locus_tag="VLA60_05710"
     CDS             complement(3640..>4617)
                     /locus_tag="VLA60_05710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSF08889.1"
                     /translation="DGSPYPREACPIHAALKEGRVYHNDTEGLWRKNGTSFPVDYYIS
                     PIRENGILIGAVVTFKDISERVRMTAELLEETKLAGVTVMLGDIAHDIKNMLMPVLNG
                     AKLLDDDLQELFAKLPDVTPKELSASRKFSSEAVDMIVKNARRIQGRMREIADTVKGI
                     SSPLRFAPCQVSEVVEGVYGILRFYATEMEVSLHTQGLDVLPPIQADENRLFNALYNL
                     VNNAIPETPAGGSVTIGGSIGLDGGTVVLTVADTGGGMPPEIRDRLFTKDAISGKAGG
                     TGLGTKIVKDVVDAHSGTVSVHSEPRKGTTFTIQLPMTPKISKPEGILP"
BASE COUNT         1279 a         1234 c         1022 g         1082 t
ORIGIN      
        1 tgaaatttgg acttcattcg tctgcgcgtc cggtcaatca acaaacgcct ctcctaacct
       61 tctccatgat attttcactg ccttactcaa ttattccaga gagaacatcc gacgggagtg
      121 ataccacaac acttcacctt agctgacgcc gtatctacca caaacattcc tcccttaact
      181 ccgtcaactc tcatcccttg gtcacgacca ggaataagat cctcattggt agaaacgaca
      241 gcatctgaaa aagctcggct caccccaaaa acacttacaa gcatcaaaca gacaattcat
      301 agatttctcc atgtcctcat aaacatccct cctacaaaat aaaacctatt tcatcgtggc
      361 catattacca agccctctgc ggctattgga tcaacatcaa ccccttatgg gcaagtgaga
      421 aacgcctcca acctgcctac cgtatggagg tctcttaccg atcaacccgg cctcctttca
      481 ctccaattat cataagaaaa gatcagacca tacaatgttg atattttctt cattgcgtcc
      541 aaactggaat aagaattttt attcctgaat gccaatccaa gtctctgacg aggcccactt
      601 aacaaaaaag ccaatagaac gaaaccctat cagataaaga gctgcagcac ttcactccct
      661 gcctgtttgg aaaaggattc attgatcttt tattgcccca ttcaaaaatt tctgttaccg
      721 tggatcacgt aactacagta atcacctcgc acatcccgct tgtaactcaa atataggacg
      781 aacgtatggc atgggaacct aaagatccgt ggggaggaag cggtcaagac cccctcgatg
      841 aagcgcttcg gaaagcacaa gaccaactca ctcgactcgt gcccggccga ggtttcaaat
      901 caatcattta catagtcttg gcagtcctgg ctctctggca atcggtgttt attgtggccc
      961 cggacgagca aggtctggtg aaacggtttg gggatattgt tcgcgaggtt gaatccggcc
     1021 cacatttcaa gattccattt gtggaaacag tggacacccc aaaagttgaa aaactccatc
     1081 gggtagaggt gggattccgc accgaccgag gacgcaccca attcgttccc aaagaagccc
     1141 tcatgttgac cggtgacatg aacattctgt ctctcgagtt catcgtccaa tataaaatca
     1201 aagaagcgaa aaattattta tataaagtcg ccgatgtcga gtccacgatt cataacgccg
     1261 ctgaagcagc catgcgggaa gtcatcggca aaagtaaaat tgatgaagcg ctaacgatcg
     1321 gcaaagccca gattcaacag gaagctcaag atctgcttca aggcgtcctc gatcagtacc
     1381 tggccggcgt acaagtggcc accatccaac ttcaggatgt gaatcctccg gaagccgtcg
     1441 ccgcggcttt taaagatgtc gccagcgcca aggaagaccg tgaaaaactc attaaccagt
     1501 ctcacggcta ccgaaacgat ctaatcccta gagcaaaagg ggaagcggct caaggcatca
     1561 accaggccaa agggagcgca cagtcacgga ttgcacgtgc ggagggagaa gccaattact
     1621 ttctccagac tttaaaagaa tacaaactgg ccaaggatgt catcagtaaa cgggtctaca
     1681 ttgaaacgat ggaagaagtc atggcgaaca cggaaaaaat tattctggac tctaaggcgg
     1741 ccggcaatgt cctgcccttc cttccgttag accgccaatc cggctcgcgc ggagcaacaa
     1801 acacgcaagg aggggactcc cgatgaaaca aaatctcctc attggtgtca tcttagtttt
     1861 tggactgctt ttggtattag gtctttctcc attttttgta gtcgacttaa ccgaaacggc
     1921 tattgtggtc gcattgggaa aacccgtcca aaccatcacg gaaccagggc tgaagtttaa
     1981 aattcccttt taccatcagg tcaccttctt tgataaacgc tatttggact atgacgctcc
     2041 cgctcgagat gtcattacca gcgataagaa atccctggtg atcgacaact tcgccaaatg
     2101 gcgcattgtg gatcccttaa aagtctatca ggcctttcag acccaacgag gtgccctcca
     2161 gcgcctggac gatattattt attcggaatt gcgggttgag ttgggccgcc atgaactcgg
     2221 tgaaatcgtg gccgcgaacc gtgcattgat catgaaggtc gtatcggatc gatccaacga
     2281 aaaagcctcg gcgtacgggc ttgaggtcct ggatgtacgc attaaacgcg cggacctacc
     2341 tgagcaaaat gaaaaagcga tatttcagcg aatgcaagcc gaacgtgaac ggcaggccaa
     2401 gcaatatcgg gcggaaggtg aggaagaagc gcaaaaaatt cgatcagaag ccgaaaaaga
     2461 caagcaaatt attttggcgg aggcctataa aaccgcccag gaaatacgtg gagacgggga
     2521 agccaaagct tataaaatct acgcgacggc ctaccaacaa ggcccggagt tttttgaatt
     2581 tatccgaacc atggaagcct ataagaaaac ctttgcgaac aatacaacgt tggttctctc
     2641 cccggattcg gaattcctta agtacctcaa gaaacggtaa agaaacaaac acccaccacc
     2701 ttcagccagg cgatccggtt cgtcgttcaa tcccgacact ctcctcatgg ccaatcctga
     2761 acatgttgaa cgcctcatca agggaggggt cggggaatgg aacaaatttc gacggacaaa
     2821 tcagacgatt caacccgatc taagcggagc cgatctttcc gggtacgact ttacggggct
     2881 tgatttaagc caggccaatc ttgaaggggc agacctctca gacgcctatc tctttcatgc
     2941 caatttaaca gaagccaacc tctccggtgt gaacctcacc gaagccgatg tgcaggaagc
     3001 caatctgatc aaggctaatc tctcacatgc caccatgacc aggattgatt tgtctggaac
     3061 cgggcttgtt gcctgcaacc tggagggcgc gaatcttgag ggggccaccc ttacacgtgc
     3121 ttcgcttcca tggggaaatc ttacccgagc caatctggcc aaagccaagc tccacctaac
     3181 tgatttgcgt gaggctcaac tcatacaggc taatctggaa ggggcttcac ttcaggatac
     3241 atccctgaca gggacgaact tacagggagc caatctccaa ggcgccactc atgtctccat
     3301 tgatctgctc gccaaagcca aatctctgta tcaaaccatc cttgatccgg acctaaaggg
     3361 aacccttctg caatgccatt ctcacctttt cgaaaaacca gttgacctat agaaaagagc
     3421 tccaagcaat ttttattgga ttcctttgga acgaataatt tacgaagttt tatccatgcc
     3481 attattgtac gtattgatgc aagtcagtaa gcattcgaaa tagtcttgtt cagcaagacg
     3541 gttggcactt tggcaggcga aaggtccaga ttcgcaggta agaataacaa tggaccaaga
     3601 tcacgtatga atggccgttc ccctgattca agaatcccgc tatggaagaa ttccttctgg
     3661 tttcgaaatc tttggggtca tcggtaattg gatggtgaag gtggtgcctt tccgtggttc
     3721 actatgcacg ctgaccgttc ctgaatgagc atcaaccaca tccttgacta tcttcgtccc
     3781 caatccagtt cccccggctt taccgctaat cgcatcttta gtaaataatc ggtcacggat
     3841 ttcagggggc attcccccac ccgtatctgc gaccgttaac accacagttc ctccatccag
     3901 ccctattgac ccaccaatgg tgactgatcc accggcaggg gtttccggaa ttgcattatt
     3961 gaccagatta tagagggcgt tgaacaaacg attctcatcc gcctgaatgg gtggaaggac
     4021 gtcaagacct tgcgtgtgca gggaaacctc catctcggtg gcatagaagc gaaggatgcc
     4081 atagacgcct tcgaccactt ccgacacctg gcatggagca aaccgtagcg ggctggatat
     4141 acctttgacc gtatcggcaa tctcccgcat gcgaccttga atccgacggg cattcttcac
     4201 gatcatgtcg accgcctcgc tggaaaattt tcttgaggcc gacaattcct tcggagtaac
     4261 gtcaggtaac ttcgcaaaca attcttgaag atcgtcgtct aacaatttgg caccattcag
     4321 caccggcatg agcatattct taatatcgtg ggcaatatcc ccaagcatca ccgtcacccc
     4381 agcgagcttt gtctcttcca ataattctgc agtcattcgt acccgctcgc tgatatcctt
     4441 gaacgtcacc acagccccaa tcaaaattcc attttcccgg atcggcgaaa tgtagtagtc
     4501 cacggggaag gaggtcccat ttttgcgcca gagcccctct gtatcattgt gataaaccct
     4561 tccctctttg agcgcggcat gaataggaca ggcctctctg ggatagggtg acccatc
//
LOCUS       DATIAL010000183        11929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1539249, whole genome shotgun sequence.
ACCESSION   DATIAL010000183 DATIAL010000000
VERSION     DATIAL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539249"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VLA60_05715"
     CDS             complement(<1..640)
                     /locus_tag="VLA60_05715"
                     /inference="COORDINATES: protein motif:HMM:NF017765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HSF08890.1"
                     /translation="MLEEFIYNFTHNLFKPLLLFFYLGFLVPILKVPFEFPHVLYKGL
                     TIYLLIAIGWHGGEELASLSMAELQHALGFMVIGFFTNLIIGIAAYVILRKTNLRRID
                     AATVAGYYGSDSAGTFVTCLGVLAAANIAFAAYMPVLLAVMEIPGCLVALYLVSRLRA
                     SGRLDALGNMPDERGYNPYARKLVPPPTEDEDNHSNHVEQIPVSLPAQSLGAA"
     gene            1113..1691
                     /locus_tag="VLA60_05720"
     CDS             1113..1691
                     /locus_tag="VLA60_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08891.1"
                     /translation="MRCWILTGILVASGFLSGIEAWGLEFTADQILKFGRQTQKANLY
                     YRDDRWRLEHQSMGPVNVTIVRKDKQLMWLLLSRVRHFKTLPYDPAQEPKLSKHLEGE
                     VSREEIGTETREGHPTILYEVTVKQGDEIDVYYQWFATDIQFPMQLTRKDGSWSVEYR
                     HVKLRPVTDYLFQLPVNFQPLEEFDPPKKKES"
     gene            complement(1894..5052)
                     /locus_tag="VLA60_05725"
     CDS             complement(1894..5052)
                     /locus_tag="VLA60_05725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSF08892.1"
                     /translation="MSAFVHLHVHSEYSPMQGMSSLETLCQTAKGQGAETLALTDTNG
                     LYGAIRFIEVAQAHGLRPILGAELIHNRHRAVLLVKDSFGYTNLCRLLSQRHCDDDFN
                     CIAAIQEHRQGLIILSDDPAALTAWKRHSPSDLYVELSPGALMHQALAFSRRTHLPPV
                     ATNRVHFVTPHEFPFHQILRAIALNTTLSQLPPEACCAPHHWLMPPAILASQFPHVPH
                     AIANTWKIAQQCQTEWSFKETIFPSFRQLSDPHAFTLLREKTYDGARWRYGSLTAVVT
                     DRIERELSVIRDKGYAHYFLVVDEIVRQAPRTCGRGSAAASIVSYCLGITHVDPIRHN
                     LFFERFLNPGRHDPPDIDVDFPWDERDRIVDFVFARYGSRQAGMVANQNTLALRAAVR
                     EVAKVYGMPPAEIQQVSRLLTRLPLTDLPAQESSISPCSQSAIRQTPRLSNIRENGKP
                     RSLPSSPFSRSHNGKKPPSHLDEASAFTDLREPWPEIVRLALQLQGRFRHLSLHCGGI
                     VIVPDDLRRYVPVEVAAKGIPVIQWEKDQTEDAGLVKIDLLGNRSLAVIRDALAAIAE
                     HTGRHIDYATWDPLTDPKTQQLIQRGETIGCFYIESPATRLLLKKLWTGMPADQRARA
                     DVFEYLVIVSSLIRPAANRFIQEFVRRAHGGAYRPLHPLVEDVLVETHGIMVYQEDVT
                     KVAMGLAGFSVEDGDQLRKILSKKHKQRRLRDYQRQFVDGARARGVDARTIERAWAMT
                     MSFTGYSFCKPHSASYAQVSFKSAYLRAHYPGEFMAAVISNQGGFYSTFAYLSEARRM
                     GLTVLSPDINASAWAYEGEGTTIRMGLMQIKGIVRGFIDRLLAERSRHGPFQSFHDFL
                     SRLNPEPAQTRLLVLAGCFDAIAGEVTRPGLLWRVYAEHPTCGISSPRSVAQHATPLL
                     PSVRLLPIPNEYDTERLIQHEIDLFGFPLRCHPLTLYARHLQNLQITPATEMAMHIGR
                     RIMMVGWLITEKAASTKHGKPMEFITLEDTTALYDATLFPEIFQRYGPLLTNDRPLLL
                     EGLVEEDFTATTLTVQQMRVIG"
     gene            complement(5334..6539)
                     /locus_tag="VLA60_05730"
     CDS             complement(5334..6539)
                     /locus_tag="VLA60_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08893.1"
                     /translation="MMDRQIVCLCIPRFEVALARLDAPSLRTRPIAVASAHTSRAIIR
                     EVSPEAEDAGVIPGLQADRARRRCPSLRLVPPNPTRVAQAHHALVSCMIPIAPIWESF
                     RPGHFYLDLTGTTRLFGATCDTTMRLERDVTSRLGLQAVAGIGTNKLVAQMASSILTP
                     PQLCDVRAGSEQDFLSPLPVSILPGLQGPGGSALHARLTDLNLQTIGDVCDVSLEALE
                     TVCGRWGHRLYQWARGIDFLPVVAPEASLSLTHSYLFEPDTIDHDRLLGGLSCLLDSL
                     CHDLRRRQRLCHRLRLTLLSSDQLTVHHSSVLPTPTHWEVDMFPSAQTLFRRCFQRRV
                     RVRSLTIRSNSFRPPPEQLSLFPLHDQEEPQALPRPHRLALALDRLHTRFGMKVIQWG
                     RSHLASRLD"
     gene            complement(6547..7089)
                     /gene="lexA"
                     /locus_tag="VLA60_05735"
     CDS             complement(6547..7089)
                     /gene="lexA"
                     /locus_tag="VLA60_05735"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HSF08894.1"
                     /translation="MTPEAFKQIRQRLGLTQTQLAERLRTTRMTITRYECGMRRIPGV
                     VEAILSQLDSPTQIPMAGIVAAGNPIEPIPQTELVEVPPSMLRTGDNFALRVKGESMR
                     DEGILPGDLVIVHKQRMARNGQTVVALVNQEATIKKYYNKEGQIELHPANATMTPILV
                     TPQDEFVIEGVVIGVIRHCG"
     gene            7438..8181
                     /locus_tag="VLA60_05740"
     CDS             7438..8181
                     /locus_tag="VLA60_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HSF08895.1"
                     /translation="MFEWLTSPEAWIALGTLTALEIVLGIDNIIFISILVGRLPESQR
                     AFARRAGLGLAMVARLGLLFSISWVMGLTEPLVTVFTKAISGRDIILVGGGLFLMAKA
                     THEIHNSLEGIEEENGHAIVAANLGMVLVQIAVLDIVFSLDSVITAVGLVDQVSLMAI
                     AIILAVLVMLMAAKAIGDFVDEHPTIKVLALSFLILVGVTLMVEGFDVHVPKGYIYFA
                     MAFSITVEMLNIRMRKKRAPVKLYKTISE"
     gene            complement(8302..8868)
                     /locus_tag="VLA60_05745"
     CDS             complement(8302..8868)
                     /locus_tag="VLA60_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HSF08896.1"
                     /translation="MSAQEANDVLERLCNLQRMEARAFGLRYGLQPVQMEALTYLTQC
                     NRYSNTPQAVAEYLGLTKGTVSQSLKVLEQKGFLRKQADNEDKRIVRLAPTAKGSNLI
                     KKALLTRSLEPALARTDSIKIAELSSALRSILRGMQQVNGRKAFGACHTCRFNEDHEK
                     NGYVCGLTREPLSVQDIQLICREHQYPH"
     gene            8970..9212
                     /locus_tag="VLA60_05750"
     CDS             8970..9212
                     /locus_tag="VLA60_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSF08897.1"
                     /translation="MKKAVLYHAGCPVCVSAETMVTQAIDRQQYDLEIVHFAQTPGRV
                     VEAETAGVKSVPALVLEGQVFHVNFGASMEDVKASI"
     gene            9302..9760
                     /locus_tag="VLA60_05755"
     CDS             9302..9760
                     /locus_tag="VLA60_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HSF08898.1"
                     /translation="MRAWVRNVCLGIALGLSMSGAACSTVEPPAGKGSASLYDRLGGK
                     PAITAVIDEFVGNVANDARINGRFATTDIPKLKGHLVDQVCGATGGPCTYTGRDMKTT
                     HAGMRITNADFTAMVEDLVAALNTFKVPPTEQDELLGLLGSMKSDIVEIP"
     gene            9939..10808
                     /locus_tag="VLA60_05760"
     CDS             9939..10808
                     /locus_tag="VLA60_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HSF08899.1"
                     /translation="MTNVLPSEMGVIQSLWRYPVKSMRGEALSLAQVTDHGLVGDRAY
                     AILDRADGKVATAKNPKKWPNMFAFQATFLEPSGDKEFRSRIRITLPDGTMVTSEQQD
                     LPQVLSKALNREVTLALVEGGKVTGVQSPMPETWIAQSEEYWPDMEGREKRDTVTDFS
                     LPTGTFFDAAMVHLLTTGTLNQLREAYPVGRFEVPRFRPNVVVDTGADEQGFIEQGWI
                     GQTVGIGKNVRLKIAGSCGRCVMTTLAQGELPKDSGILRTAVQHNLGNVGVYASVIQG
                     GTIRIGDGIRLEK"
     gene            11093..11671
                     /locus_tag="VLA60_05765"
     CDS             11093..11671
                     /locus_tag="VLA60_05765"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HSF08900.1"
                     /translation="MTFPLITDPKKKLLSIIAFFLLLYVMVALTGLRSHLSPDVIQSL
                     FFAYPVWGLVLFCLAFSFGNLLYVPGWIFLVGAVFALGKEWGGVATLMAAMCSSTISF
                     LLIRAVGGTALRSFNHRWADTIFSHLDERPILSVAFLRLLFQTLPALNYALALADIRF
                     RDYLVGTAVGLPLPIFLYCYFFELIFQHLLNR"
BASE COUNT         2800 a         2826 c         3386 g         2917 t
ORIGIN      
        1 ctgctgcgcc cagcgactgg gccggcaaag aaaccgggat ctgctcgaca tggttgctgt
       61 gattatcttc gtcttcagtt ggggggggga cgagtttgcg ggcatagggg ttgtatcccc
      121 gctcatccgg catattcccg agggcatcca gcctgccgga ggcccgaagc cgcgatacga
      181 ggtaaagggc caccaggcag ccggggattt ccatcacggc aagaagaacc ggcatatacg
      241 ctgcaaaggc gatattcgcc gcagccagca ctccgagaca ggtgacaaaa gtccccgctg
      301 agtcagagcc ataatatccg gctacggttg ccgcatcaat tcgacgcaaa ttggtttttc
      361 gaaggatgac ataagcggcg atgccgatga tcagattggt gaaaaaccca atcaccataa
      421 acccaagagc atgttgcaat tcggccattg agagagaggc cagttcttcg cctccgtgcc
      481 aaccgatggc gatcaaaagg taaatggtta atcccttata gaggacgtgt ggaaattcaa
      541 acggtacttt gaggatgggg actaaaaatc caagatagaa aaatagcaac aagggtttga
      601 acaaattatg ggtaaagttg taaataaatt cttcaagcat acatcgctcc tttaactatg
      661 aagtctgcat agcatgttca tggattggtt gagatttttg atatcttctt aagccttctg
      721 agaaaaaatg gttttattat cctgccatct aatatttgac cttcagcgtc tttttgcaaa
      781 attaggtcca tgattaccca ttgagatgtc atcacagaaa gatctgccca aacccagtaa
      841 acacaggtgc tgtgccatcg agatgacttt ttaatccttg gtatgggggt ttgacttctt
      901 cccatctgag cgaaatcgca acccgcgcac tgttcaaatt cactattttg aaaataacgt
      961 aggcagaaat ctttgaagag acctacggct gaaaccaggt tcattgaaat ggacaaattg
     1021 gagaggagaa tattcctcat aagaagatta tcctgggtgc cacggtcaat aaatttttgg
     1081 ggcaccagac tcgaatatgg tagtatgagc cgatgcgttg ttggattctc accgggatac
     1141 tggtcgcgag tggcttcttg tcaggcattg aggcttgggg actggaattt actgcagatc
     1201 aaatcctgaa gttcgggcgg cagacccaaa aggccaatct gtactatcgg gatgaccggt
     1261 ggcggttgga gcatcagtcg atgggtccgg tcaatgtcac cattgtgcgt aaagacaagc
     1321 agctcatgtg gctgctgctc tcccgcgtga gacacttcaa aacgctcccc tacgatcctg
     1381 ctcaggagcc aaaattgagc aagcatctgg agggagaggt ctctcgcgaa gagatcggca
     1441 cggagacgcg cgagggtcat ccgacgatct tatacgaggt cacggtgaag cagggcgatg
     1501 agatcgatgt ctactaccaa tggttcgcca ccgacattca gtttccgatg cagttgacca
     1561 ggaaggacgg aagctggagt gtcgagtatc gacatgtgaa gctgcggccc gtaaccgatt
     1621 atttatttca actgcccgtc aatttccagc cgctggaaga gtttgacccg ccgaagaaaa
     1681 aagaatccta agagcctgcg atgtcgtcac ctatccgttc gaaggcaggg tggcccctgc
     1741 cggctacaac atctcctaac aaataaaata acttctctgc ctgaacatag acccttcgcc
     1801 gttggggtca gggtggcaat gtggtgtggg tttgaccagg atgtggtcgg cccatgctta
     1861 aatggtgaaa tctatgtttg gacagcttgg gtgttaaccg atcacgcgca tctgctgcac
     1921 cgtcagtgtg gtggcggtga agtcttcctc caccaatccc tccagaagaa gcggccgatc
     1981 gttcgtgagt aacggtccgt atcgttggaa tatttcgggg aacagcgtgg catcgtaaag
     2041 tgccgtggta tcttccagcg taataaattc catgggtttg ccatgtttcg tcgaggcggc
     2101 tttttccgtg atcagccagc caaccatcat gatccgccgg ccgatatgca tagccatctc
     2161 tgttgctgga gtgatttgca ggttttgcaa atgtctcgcg tagagcgtca acggatgaca
     2221 acggagaggg aagccgaaga ggtcaatctc atgctggatc agccgttctg tgtcatattc
     2281 attcggaatc gggagcagac gaacggatgg caacagcggg gtggcgtgtt gtgcaactga
     2341 tctgggtgag gagatgccac aggttggatg ctcggcgtag acccgccaga gcaggccggg
     2401 gcgcgtgacc tcaccggcaa tggcatcgaa gcaacctgcc agaaccaata accgcgtctg
     2461 cgccggttcg gggttgagtc tgctcagaaa atcatgaaac gactgaaacg ggccgtggcg
     2521 agatcgttct gcgagaagcc gatcaatgaa tccccgtacg atgcctttga tttgcatcaa
     2581 gcccatgcgg atggtcgttc cttctccctc atatgcccat gcgctggcat tgatgtccgg
     2641 ggagagcacg gtcaatccca tccggcgggc ttctgacaga taggcgaagg ttgaataaaa
     2701 cccaccctgg ttgctgatga cggcggccat gaattcccca ggataatggg cacggagata
     2761 ggccgactta aatgacacct gcgcataact ggccgaatgc ggtttacaaa aactatagcc
     2821 ggtaaaactc atcgtcattg cccatgctcg ctcgatcgtt cgcgcatcca caccgcgcgc
     2881 gcgcgcgccg tcgacaaatt ggcgttgata atcgcgaagc cggcgttgct tatgtttttt
     2941 actcaggatt ttcctgagtt gatcgccatc ttccacggaa aaccccgcca gacccatggc
     3001 cactttggtg acgtcttcct gatataccat gatgccgtgg gtttccacga gcacgtcttc
     3061 cactaacgga tgcaaggggc gataggcgcc gccatgtgcc cgccggacaa attcttgaat
     3121 gaaccggttt gccgcgggac gaatcaatga tgagacgatg acaagatact caaagacgtc
     3181 ggcgcgtgcc cgctgatccg ccggcatgcc cgtccagagt tttttgagaa gcagtcgggt
     3241 ggcaggcgat tcgatgtaaa aacatccaat tgtctcaccc cgttgaatga gctgttgcgt
     3301 tttggggtcg gttaatgggt cccaggtggc atagtcgatg tgtcggccgg tgtgttctgc
     3361 aattgccgca agggcatccc gaatgacggc taaggatcga ttgcccagga gatcgatttt
     3421 gaccaggccg gcatcttccg tttgatcctt ttcccattga atgaccggaa taccctttgc
     3481 cgccacttcc accgggacat agcggcggag gtcatcaggg acaatcacaa tacccccgca
     3541 gtgcagggac aggtgacgga accgtccctg aagttgaaga gcgagtctga caatttccgg
     3601 ccatggctcg cgcaaatcgg taaacgccga ggcctcatca agatgtgagg ggggtttttt
     3661 gccattgtgt gacctcgaaa acggcgaaga tggaagagag cgaggcttcc cattttctcg
     3721 aatattggaa aggcggggag tctgccggat cgcagattgg ctacatgggg agatagagga
     3781 ttcttgagcc ggaagatcgg tgagcgggag acgggtcaac agtcgactca cctgctgaat
     3841 ttccgccgga ggcatgccat agactttggc gacttcccgc acggctgccc gaagcgccag
     3901 tgtattctga ttggccacca tgccggcctg ccggctgcca tagcgcgcaa agacaaaatc
     3961 cacaatgcgg tcgcgctcat cccagggaaa atccacgtcg atatccggcg gatcgtgacg
     4021 cccgggattt aaaaagcgtt caaagaacag attatgccgg atgggatcca catgggtaat
     4081 ccccaggcaa taggacacga tggatgccgc cgccgatccc cggccacagg tgcgaggcgc
     4141 ctggcggaca atctcgtcaa ccaccagaaa ataatgggcg tagcctttat cccggatgac
     4201 gctcagctcg cgttcgatac gatcagtcac gaccgctgtc agtgagccgt accgccatcg
     4261 ggccccgtcg taggtcttct cgcggagcag agtgaaggca tgtgggtcgg agagctggcg
     4321 aaaactcgga aaaatcgtct ctttgaacga ccactcggtc tggcactgct gcgcgatctt
     4381 ccaggtattg gctatcgcgt gaggcacatg gggaaactgt gaagccagta tcgcaggtgg
     4441 catcaaccaa tgatggggtg cacagcaggc ctccggcggt aattgagaga gggtggtatt
     4501 taaggcgatc gcgcggagaa tttgatggaa ggggaattca tggggggtca cgaaatgcac
     4561 ccggttggtc gcgacgggag gaaggtgtgt gcggcgacta aaggccaggg cctggtgcat
     4621 gagtgcgcca ggcgagagct ctacgtacag atcgctaggt gagtggcgtt tccaggccgt
     4681 cagcgcggca gggtcatcag agagaatgat cagtccttgg cgatgttcct gaatcgcggc
     4741 gatgcagtta aagtcgtcat cgcaatggcg ttgggagagc aggcggcaga gattggtata
     4801 gccaaaagaa tctttgacca ggagcacggc gcggtgccgg ttgtggatca gttcggctcc
     4861 caggatcggt cgtaagccat gggcctgtgc gacctcgata aagcgaatgg caccgtacag
     4921 gccattcgta tcggtcaggg caagtgtctc ggctccctgt ccctttgccg tctggcacaa
     4981 ggtttccagt gaggacattc cctgcatcgg ggagtactcc gaatgaacgt gtaaatggac
     5041 aaaggctgac atgaaataat gcggcaataa tgataaattg cgtacccatt tttaattaaa
     5101 gtatcgcttt actttatatg catgttgata acctgttgat taaagtaatg ccttattttt
     5161 tctgcctgtg cataaatcaa tcattaaagt ttttgattaa tttttatcat tttaaaatgc
     5221 aaaacaccca ttattcattg cttattttgc atattaaata atggcaaaag aatgagggtg
     5281 atcatgccac agaggtggga gcatcgcttg agaattttta aaaactcact tattcaatct
     5341 aaacgagagg ccaggtgtga gcgtccccat tgaatgactt tcatgccgaa ccgggtatgc
     5401 aaacgatcaa gggctaatgc caatcgatgg gggcgtggga gtgcctgagg ttcttcctgg
     5461 tcatgtaatg ggaagagcga tagttgttcg ggtggaggac gaaagctatt acttcgaatg
     5521 gtcagtgagc gcacgcggac gcgtcgttgg aagcaccgac ggaataaggt ctgagctgat
     5581 ggaaacatat caacctccca atgtgtgggt gtgggtaaga cagatgaatg gtgaaccgtg
     5641 agctgatcac tagagagcag cgtcagtctg aggcgatgac acaggcgctg tcgccgacgt
     5701 agatcatggc agagagaatc cagaagacat gacaggcctc caaggagccg gtcgtggtca
     5761 atcgtatccg gctcaaagag ataggaatgg gtcagcgata acgaggcctc aggagcaacg
     5821 acgggcaaaa aatcaatccc gcgtgcccac tgatagagcc ggtggcccca tcgaccgcag
     5881 acggtttcca aggcttccaa actcacgtca cacacgtcgc cgatggtctg tagattcaaa
     5941 tcggtcaagc gtgcgtggag tgcggaccct ccaggacctt gtagcccggg aagtattgag
     6001 accggtaaag gcgataaaaa gtcctgttcg gagcctgccc ggacatcaca taattggggc
     6061 ggcgtgagga tggagctggc catttgcgcg acaagcttat tggtcccgat cccggcgacg
     6121 gcttgtagcc caagtcgcga tgtgacatcg cgctctaaac gcatcgtggt gtcacaggtt
     6181 gcgccaaaga gtcgagtggt gccggtcaaa tctaaataaa aatgcccagg cctaaacgac
     6241 tcccaaatgg gtgcgatcgg tatcatgcac gacacgaggg catgatgggc ttgggcaaca
     6301 cgagtagggt tgggaggcac gagtcgaagc gagggacagc gtcgtctggc ccgatcggcc
     6361 tgaagtccgg gtatgactcc ggcgtcttca gcttcgggag aaacttcacg gataatggca
     6421 cgggacgtat gggcggaagc cacggcgata ggtctggtgc gtaaggaggg ggcgtcgagg
     6481 cgtgccaggg ccacttcgaa tcgaggaata cataaacaga caatttgacg gtccatcatt
     6541 tttcatctag ccacaatgcc gaatgacccc gatcaccact ccttcgatca caaattcatc
     6601 ctgcggagtc accagaatcg gagtcatggt tgcgttcgcc ggatgcagct ctatttgccc
     6661 ttctttgttg taatatttct taatcgtcgc ctcctgattc accagcgcga ccacggtttg
     6721 tccatttcgc gccattcgtt gcttgtgtac aatcaccagg tcacccggca ggatgccttc
     6781 atcccgcatc gattcacctt tcactcgtaa tgcgaaattg tctcccgttc gcagcatgga
     6841 aggtggaacc tccaccagtt cagtttgggg aatgggctca atcgggttgc cggccgcgac
     6901 gatgccggcc atgggaatct gtgtcggaga atccagttgg gagaggatgg cttcgaccac
     6961 tccgggaatc cgacgcattc cgcattcata gcgggtaatg gtcatacgag tggtgcggag
     7021 acgttcagct agttgggttt gggttaatcc caggcgttgc cggatctgtt tgaaggcttc
     7081 aggtgtcatg taaccaatgg ttacattgtg acatgagagg tgtcaagaga ggtggttgcc
     7141 accgacccac tactcatgac tacatcgagt gcaaatattg taagtgtttg attattaatt
     7201 attttttaat aaatcactca gtttccggga atatcactct taggacagct catcgtggga
     7261 aggacaggtc agcactgttt cctgttcacg aaccaagaag tgatcaggat ccaccctaga
     7321 aaaaggcgca catgttcatg ccggcgcatc ctgaacggtc gcacatcgct atggaggaat
     7381 gttgctgccg gcatggctca acgcattcga tgatcaaaga aagaaaagga gaatctgatg
     7441 tttgaatggc tgacaagtcc tgaagcatgg atcgcattag gcacgttaac cgcgttagaa
     7501 attgtcctgg ggatcgataa cattatcttc atctccatcc ttgtcggacg ccttcccgag
     7561 agtcagcggg cgtttgccag aagagcaggg cttggcctcg ccatggtggc acggctggga
     7621 ttgctgtttt cgatttcctg ggtcatggga ttaacggaac ctttagtgac ggtgttcact
     7681 aaggcgattt cagggcgcga tatcattctg gtcggcggcg ggctgtttct gatggcgaaa
     7741 gccacgcatg aaatccataa cagcctggag ggtatagaag aagagaacgg tcacgcgatt
     7801 gtggctgcca atttgggaat ggttctcgtt cagattgcgg ttttggatat tgtgttttca
     7861 ctggattcag tcattacggc ggtcggtctg gttgatcagg tttctcttat ggcgatcgcc
     7921 atcatcctgg ccgtactcgt gatgctgatg gccgcaaaag cgattggaga ttttgtcgat
     7981 gaacatccga ccatcaaagt tcttgccctc tcatttctca ttctcgtggg tgtgaccctg
     8041 atggtggagg ggttcgatgt gcatgtcccg aagggctata tttattttgc gatggcgttt
     8101 tccataacgg tggaaatgct caatattcgc atgcggaaaa aacgggcccc ggtcaagctg
     8161 tacaagacga tttccgaata gccggaaata cgcggacgcc acaggacgcg tgggaggtcc
     8221 ttcgcggaag aggggagagg agataatttt ggccacccgt ctgtgtgggg agaagtcccg
     8281 gatgatgtgg tgcaagatgc gctaatgcgg atattggtgt tcgcggcaga tcaattgaat
     8341 gtcttgcaca cttaacggtt ctcgtgtcaa accgcagaca tacccgttct tctcatgatc
     8401 ttcattgaaa cgacaggtgt ggcaggcacc aaaggctttt cgtccattga cctgctgcat
     8461 tcctctcagg attgagcgca atgcagaaga cagttcagcg atttttattg aatccgtcct
     8521 cgccagtgcc ggttccaggc ttctggtgag aagggccttt tttatgagat tgctcccttt
     8581 ggcggtcggc gcaaggcgta cgattcgttt gtcctcatta tccgcctgct tccgcaggaa
     8641 tcccttctgc tctaagacct tgagcgattg tgagacggtt cccttcgtga gacccagata
     8701 ctccgccacg gcttgcggcg tatttgaata gcgattgcat tgcgtcagat acgtcagcgc
     8761 ttccatctgc acgggttgta atccgtaacg caggccaaac gcccgtgcct ccatccgttg
     8821 caggttgcac aatcgttcta agacatcatt ggcttcttgt gcgctcatag gtcaattgta
     8881 tcgtctcgat acagatttga caaatacgga tatcgctgct aaattccatt tggtttcgac
     8941 tcaatactaa tttcaaaagg agacagttta tgaagaaagc cgtgctttat catgcagggt
     9001 gcccggtctg tgtttctgcg gaaaccatgg tgactcaggc catcgatcgt cagcaatacg
     9061 atctggaaat cgtccatttt gcacaaacac ccggtcgggt ggttgaggct gaaacggcag
     9121 gtgtgaagtc tgttcctgcc ctggtgttag aggggcaggt atttcacgtg aattttggtg
     9181 catccatgga ggatgtgaaa gccagtattt aaggcgaaag actttattct cgggagtaag
     9241 acgggttgct aggggtagcc tgaacgccaa ctcccggtta tcaacgaaag gagtaaatcg
     9301 tatgcgtgca tgggtgagga atgtgtgtct cgggatcgct ctagggttgt ccatgagtgg
     9361 ggcggcctgt tcaacggttg aacccccggc gggaaagggg agtgcatcgt tgtacgaccg
     9421 gttaggggga aagccggcta tcacggcggt catcgatgaa tttgtgggca atgttgctaa
     9481 cgatgcccgt attaatggtc gctttgctac aaccgatatt ccgaaactga agggacatct
     9541 ggtggatcaa gtctgtggag caactggtgg gccctgcacc tataccggca gggacatgaa
     9601 gacgacgcat gcgggaatgc gcattaccaa tgccgacttt acggctatgg ttgaggacct
     9661 ggtcgcggca ctcaatacat tcaaggttcc gccaacggaa caggatgagt tacttgggct
     9721 gctcggttcc atgaaatctg atattgtcga aattccataa tcctacctcg gataattggt
     9781 tctgtgatag tcttgggggg atgggcctca tcctcccaag taccctggtt cttccttgat
     9841 agagcatacc ggtggggaat tgtttcagag tcagccattc ctgagaatgg ctgcgtgccc
     9901 gtcccgggag atcactattc aaaaaaggag agtgagaagt gacaaacgtt ttgccatccg
     9961 aaatgggagt cattcagtcg ttgtggcgct atccagtcaa gtcgatgaga ggagaggcct
    10021 tgtcccttgc tcaggttact gatcacggac ttgtcggaga tcgtgcctat gccatcctgg
    10081 atcgtgccga tggcaaagtg gccacagcca aaaatcccaa aaaatggccc aatatgtttg
    10141 cctttcaggc aacattcctg gagccgtctg gagataaaga attcagatct cgcatccgca
    10201 tcacgctgcc tgacggcaca atggttacca gtgagcaaca ggatctgccc caggtccttt
    10261 ccaaagctct gaaccgtgaa gtcaccctgg cgctcgtcga gggtgggaag gtgaccggcg
    10321 tccaatcacc catgcctgaa acctggattg cgcagtctga ggaatactgg cccgacatgg
    10381 aaggtcgcga gaaacgggac accgttacgg atttctctct tccgaccggc acgtttttcg
    10441 atgccgcgat ggtacacctt ttgactacgg gcacgcttaa tcagcttcgt gaggcgtatc
    10501 cggtagggcg ttttgaggtg ccgcggtttc gcccgaacgt tgttgtggac actggagccg
    10561 atgagcaagg ttttatcgaa caggggtgga ttggtcagac ggtaggcatt gggaagaacg
    10621 tgcggttgaa gattgccggc tcatgcggtc ggtgtgtcat gacgacgtta gcgcaaggag
    10681 aacttccaaa ggactcaggg attttacgca cggcggtcca acacaatctt ggcaatgtcg
    10741 gggtctatgc ttcagtcata cagggcggca caattcgaat cggagatggt atacgattgg
    10801 agaaatgaat gattgtgaag ttatgtctgc ctgtgttggt gtgaaagagc atcaacgcca
    10861 tcggattcgt gttgggtgta tcaggagccg gaaatttccc gtttgcctct gtaacggttg
    10921 acgaagtcag agcttattga ggggtgaccg cgaaggtttt tgagctggcc atgtcaacgg
    10981 gttcaagtgt atctcctgtg ttgggattgg tcttttttct ctcatttaac ccgcttgttg
    11041 gtatccgtgg tttttgagtt tttcagcatg catccgtaag atgcgtgtgg taatgacgtt
    11101 tcctcttatc accgatccta agaaaaagct tctttccatc attgcgttct ttctcctgct
    11161 gtatgtgatg gttgcactga ccggcctgcg atcgcaccta tcgcctgatg tcattcagtc
    11221 attatttttt gcgtatccgg tttggggtct tgtgctgttt tgtctggcct ttagcttcgg
    11281 caatctgttg tatgtgccag gatggatctt tttagtcggg gccgtctttg cgttgggtaa
    11341 agaatggggt ggggttgcga ccttgatggc cgcgatgtgt tcttcgacga tcagttttct
    11401 cctgattcgt gcggtgggtg gaacggcgct tcgcagcttt aatcatcgat gggccgacac
    11461 gattttttcg catctggatg agcgtccaat cctatcagtg gcgttcttgc gcctgctgtt
    11521 tcagaccttg cccgctctaa attatgccct ggcccttgcg gacattcgct ttcgcgatta
    11581 tctggttgga actgctgtag ggttgcccct tcctattttt ctgtattgtt atttttttga
    11641 actgattttt cagcatcttc tgaataggta agcgggcgtc gaaatatgag gaatgtaggt
    11701 aaagagggaa aaaggtttca caaagacttg taaggcttgc tcactggtaa cgaatgattt
    11761 ccccagattc aaattcctct cctttgtaag gagaggatag gtggggtaga gtccgtgggt
    11821 tatcagccaa caaccaccgg gtcagacatt gaagacacaa cgatgattac tgaatttatc
    11881 gtgacggagg gtgaaacccg caagcgcctt gatcaatttt tggtcaacc
//
LOCUS       DATIAL010000184        19860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1324098, whole genome shotgun sequence.
ACCESSION   DATIAL010000184 DATIAL010000000
VERSION     DATIAL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 19860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19860
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324098"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VLA60_05770"
     CDS             complement(<1..570)
                     /locus_tag="VLA60_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HSF08901.1"
                     /translation="MSAEQSPLHEPPPNPFGGKTLIVDAEHPDCFTLPSEALAHAQQD
                     DLIFIRPGVYEDRLVMTERTIHLVGAGRDQVTIFNRRSGPCYLQRVSGGQISGITFRY
                     VGSDQHSALNILDSVCTISHCRATEGLLSGVVIYGPDCRPTLLDNEISHNRESGIFSF
                     AGAQPYLRENTCFGNHHFGMAARDEGTRPD"
     gene            1393..1731
                     /locus_tag="VLA60_05775"
     CDS             1393..1731
                     /locus_tag="VLA60_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08902.1"
                     /translation="MFTRPDSRDAPHLFGLGIIEMLADEISQDLRQTRDQAIAKAKGR
                     RQSVEVSLKSKGISYGNIRVNPDGSVDPSRLEGGDEDLGYAHFFIMAAPFPSGNLLSG
                     HWMEKWVWRL"
     gene            1739..2575
                     /locus_tag="VLA60_05780"
     CDS             1739..2575
                     /locus_tag="VLA60_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HSF08903.1"
                     /translation="MAKAAKGGEVVTPSGMVLNGKIDTIEAPPALCESDDPDRDGVAN
                     EIPVGLVDHLEFYLLNYFKPGIGRQTRDTEQGQKLFQDIQCTGCHIQNLVIEKDRRAA
                     DVETSFDSRRGIFNDLFAVATPLFQEVPGSGSPSVKQPKGQRFVVKNIYADFKRHNLG
                     PNFWERNWDGTLTKELMTEPLWGVGTTAPYGHDGRSIDLWSVIMRHGGEAQNARDRFA
                     RLPKVKQGQIIDFLQTLVLFPPDDTASNLNPGDSQSHNFPQYEHGNIALPSLFNDPSD
                     LE"
     gene            complement(2917..3585)
                     /locus_tag="VLA60_05785"
     CDS             complement(2917..3585)
                     /locus_tag="VLA60_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08904.1"
                     /translation="MKEQRVLTHDEKKASEAAFRGLPMNDSWTDSAKAVYEGLLQALG
                     KTPLNDLNPSEKVHNQSPERSDLLPSNFSEPSQSGLSLSSGDIPAEFESLPIRSRKEA
                     IESGILKDVTPIAQSVGLDFCVGMTNPLWDQYITPSSDYTEELIQTRLRDTLVAVRLR
                     LAILKTPTPLVDVPILLPFPPEPTPQLCLTFALFHKDPVDGDCLLLIHPGEVFATRQA
                     FENN"
     gene            complement(3768..4139)
                     /locus_tag="VLA60_05790"
     CDS             complement(3768..4139)
                     /locus_tag="VLA60_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HSF08905.1"
                     /translation="MKPLFTRGEMESAIRNAIIKFEQEFMGRGPTDVRASILRDTILI
                     RLKGVLTPAERQLAKSEEGIGMVKQMRQNLISQGREQLCREILEITGAETTALFTDID
                     TRIEERIIVLTLDRDLEAIFR"
     gene            4657..7695
                     /locus_tag="VLA60_05795"
     CDS             4657..7695
                     /locus_tag="VLA60_05795"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HSF08906.1"
                     /translation="MNRLEQDRSTMTNWKWLREIVDEACQPIGPYWPMKTFAYYNPIR
                     DLEHLPFVRAIEEANQLLGAKGFLPVEEYRQIFYQGRITLPALERALRRVGPPLPARA
                     TIRVGARTIHVHDVWRIHAAYGIEILPAVLMTWTLELEGLTTRFRSDLPDDSRSSIID
                     RTIRECEKCRHDPESAYLHNLWKSSLAACQLSDPVSGLPSFHAPDPGWSAKASGYKVG
                     YKVALVPVDLPMDRTLGDWLDSETGSVLVETINTHMIKWIGAFVDEGVAGWSMPSRDK
                     GFYGAWRELAEGDLSGRFLGIPDLRQKFGELSEVPEEMLCKHLEDLKIPKERWQNYLS
                     RHLAQLPGWAGFIRWRSDHTGYSAQQHYPIDPLQYLAVRLFYESGMVEGLCQREWGIK
                     GTLPALLAYWNELREREQALSLPFSHATDPNHHAVCHQAWRLFHLAQFLELTPIEVHD
                     LSYTDMSTLLEWLDLFPQSAHGPVWLEAYEDVYRENLLRNIRGHQGVAPVNQERPRAQ
                     GIFCIDARSESFRRHLEAQGPYETFGYAGFFGVPMSHVAFDSHDHLALCPILLTPNAE
                     VTEVPRVGQNDRVKDYLSGTRWHQLSHHLFHDLKHNPFASFMLIDVLGMFFSVGLVGK
                     TLFRTSFDAVKQWLQQWLGGTVVTQIPVEASHENEQGNPQLGGLALGFTPFEQAAFVE
                     GGLRVIGLTKNFGRFVMICGHGSQSENNPYYAALDCGACGGSHGDPNARVFAAMANNP
                     EIRKILSDHGLVIPEDTWFLPAKHNTTTDRVTMYDLVDVPATHVEDLALLVRDLEQAG
                     THQALERCQRIPGAPTTVSPRDAFKHVRQRSMDWANSRPEWGLSGNAAFLIGRRALTK
                     GLDLDGRVFLHSYDPGPDLDGSLLEKIMTAPLIVGELINLTYYFSSVDPWAYGGGSKV
                     IHNMVAGVGVMLGSQSDLQKGLPLQSVNDGARHYHEPMRLLAIIEAPPDRIRSIIQKH
                     ALLQHVFHNQWMNVIAFDPESKSISRYLSDLTWEPATMNI"
     gene            8107..8598
                     /locus_tag="VLA60_05800"
     CDS             8107..8598
                     /locus_tag="VLA60_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HSF08907.1"
                     /translation="MKSSFPKQFIANTMALFCGWLVLSGKYDNFHLVLGFTASCGVAW
                     LNTGFPHSPFQQFPWGRMVLYSPWLFLRIVESSLHLTKLILNPSLPIKPRLITYRSQL
                     KHRGAIVVLGNSVTLTPGTITVEINGNTFLVHAIDEAAGNDLTTGRMERKLAWVFQED
                     TNL"
     gene            8598..8864
                     /locus_tag="VLA60_05805"
     CDS             8598..8864
                     /locus_tag="VLA60_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HSF08908.1"
                     /translation="MIDFFLFLLLTLSFLILVYLYRVIQGPTVFDRVLGLAGISTKAI
                     ILGVVIGTVYDRVEMFVDISTGYALLNLVGAFAIAKFLEQRGAP"
     gene            8865..9164
                     /gene="mnhG"
                     /locus_tag="VLA60_05810"
     CDS             8865..9164
                     /gene="mnhG"
                     /locus_tag="VLA60_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HSF08909.1"
                     /translation="MVVIAIGLILVGVFFLVVAAIGTLRLPDVFSRSHAVSLTDSLGA
                     ICVLAGLALYQGFGINMLKILVVLVLLILLNPVIAHATIRAAYRSGLKPQTEEDQ"
     gene            9161..9694
                     /locus_tag="VLA60_05815"
     CDS             9161..9694
                     /locus_tag="VLA60_05815"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HSF08910.1"
                     /translation="MMYSVNIALLLLLVLTAASATFLVKELMSSVFILGSYSFFLSLV
                     WAWLGAVDVAFVEAVIGAGLGTVLFLVTLFDTAPKDSRLRRPPPPLAGVLGLPLLGLL
                     LLLAANDLPQFGDPTSPPNVHISPVYLQNSYADTLTPNVVTSVLMDYRAFDTLIETVV
                     IFTAGIACALLLREPGS"
     gene            9691..10140
                     /locus_tag="VLA60_05820"
     CDS             9691..10140
                     /locus_tag="VLA60_05820"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HSF08911.1"
                     /translation="MKQMHDSIIVQAMSRLLIPLAQLFAFYVLFFGQYGPGGGFVAGV
                     IWTTSMILTFLVFGLKSPQGRLVEKVLHGDGIGLIIFVGVGGLCLIGGGEFLNYANLE
                     IPGLDAPARRYTGILLAQIGVAVDVAVTGISIVLSLAYHDTEQEYDV"
     gene            10130..10510
                     /locus_tag="VLA60_05825"
     CDS             10130..10510
                     /locus_tag="VLA60_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HSF08912.1"
                     /translation="MMFEMIGGWLQRPNYIAFVLLFLWGIYIMVTRYNLVKKLIGMYL
                     MQTSVIFFLVTTSAKQGATVPILLSTTEIIQPEVYVNPLPHVLTLTAIVVGVATLGVS
                     LALADAIYKKYGSLDEEEILKRLE"
     gene            10507..11976
                     /locus_tag="VLA60_05830"
     CDS             10507..11976
                     /locus_tag="VLA60_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSF08913.1"
                     /translation="MTRHLPAILFLLPLGLAICLPLIGIKHREWCRPLSIGVLAAMVP
                     VSFANLVGVLQNGTIHYDFGGWAAPVGIEWVADGLASVMTVALSLVGLVCITFLSSVL
                     FPYLGSRIVPFYTLVLLLIASLTGMVFAGDLFNLFVFLEVSALCAYALVGLAGGKALV
                     AGFRYLILGTFGASLYLLGVGYLYAETGTLNMADLAQRVPSLVASKAFAVGVLFMFIG
                     LGIKMALVPLHGWLPDAYTYAPDRVSPMLASLVTKVALYGWIRIMFWVLGAQAVVYQI
                     PILFLVGVLGALAALVGAFLALSQVEIKRMFAYGGLSHIGLILVGISLGNQTGFVGGV
                     FYLLNDAVMQAGLFFWAGAIIHLYGVRTIEDLGRLQGGAGWMSVALVVIALSMIGIPP
                     TGGFFGKWYIILGAVEAQNYLAIVAVLIATLLTLAYFVKLFERIFRDRSTLPPPIIRE
                     IPLAVKIGLGATSVAVIVLGLMSDHIVSFLLKNAVPPGL"
     gene            12007..14184
                     /locus_tag="VLA60_05835"
     CDS             12007..14184
                     /locus_tag="VLA60_05835"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HSF08914.1"
                     /translation="MTFLILIPLLPLMAVLVLVLGKPWLGEQGHKIGIPAMALSFAFS
                     AIAFARVASDGAFSIPLYRLFKSGDLVVDLGLYVDQLTVLLLLLVTGVSSVVHVYASR
                     YMIGDPRYNRFFAVIALFTFSMIMLVMSNNLLMLFVFWEIMGICSYLLISHWGERQSS
                     AQAATKAFLVNSVADVGLGFGVILTYSTFGTLDIQTILGEAPSIQGNTINLFGWLGLD
                     VPIHILTLITLFLFLGAMGKSAQVPFHVWLPFAMEAPTPVSALIHAATMVNAGPFLLV
                     RMSPLVILSPEAMTLIALVGGVTALFATLVSLTQVDIKRMLAFSTIGQIGFMIMTCGV
                     GAFVISIFHMLAHGCLKGFLFLSTGDALRSVGSQEHVSSSHVPHGSSMRSMGPLVLGA
                     LLLACLPPFLIFFGPYEQLWMIQPGSSAQWTFWILGFLTVFFSAMYVMRGVLLLFGQP
                     YSIGHGSGMGRQAIQPRFFSPLHLLGILAVMGILGSLLLVLWSWFSEFLSPVVGNRMA
                     QIPRDTSSETGLQLIFAFAVAVGGIAVGYFSLTKFSGSWFQQSEMSKRWYVHFLNKLY
                     FDEIYEAYVVVPTLRLARWLWRVIDRNVFDALIMGIANVSVLAAKWMWRVVDLRGIDG
                     LVVGLGRNSVGMAGWLWRVVDLRGVDRVVVGIGQRSIGIANWLWKRIEIKVLDKNVVR
                     AGNQAASTGNMMQELEPRTLQHHLLVMIFWLIVGIGLLFWFVV"
     gene            14320..15828
                     /locus_tag="VLA60_05840"
     CDS             14320..15828
                     /locus_tag="VLA60_05840"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HSF08915.1"
                     /translation="MNTIFTEHILSIMIAMPFLGIGIIACIQDQEWIRRVALGCTLGD
                     VGLALVLLKEFDFSTQGMQFVERMAWMPTFNIQYAVGVDGISLLLVLLTTLLCPFCVL
                     CSWKSIATRVRSFMAMILLVEGAMIVVFTALDLFLFFMLWEVTMIPMYFMIILWGGPQ
                     RIAAGIKFVLYSLSGSLILLLAILGLYVEGGRTFDILALAENTYSPDAQFWIFLAFFL
                     AFAIKLPMLPFHTWLPDAHSEAPTAGSVLLAGVLLKMGGYGFLRFCLPMFPEASVTFA
                     PFILWLSVAAIVYGGYMALAQSDLKKLVAYSSISHMGFVTLGIFVFNNHGIQGAILQM
                     VNHGITTGALFLAVGQLYDRTHSRSIQDYGGLQKSMPRFVALFCLFSVASFGLPGTCN
                     FIGEFLVLVGTSFESYVMVLLSMGGIVLAAAYMLWMLQRVVLGQPKNSTIAQLPDLNM
                     RELVTVIPLAVLILGIGLYPSPLMEMMESSVTHLAQHMAQYQVGMIQEVSLR"
     gene            16299..17378
                     /locus_tag="VLA60_05845"
     CDS             16299..17378
                     /locus_tag="VLA60_05845"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF08916.1"
                     /translation="MKLGNTLLAAVACTIVVGCVSQQSHERALQEYEKNQESTIAELE
                     RLQQAVEEKQAEVLQLQETKTALEHTVEVQDQIKEEQLAAVQQEVEALYTQIRKLVNS
                     EGTVTLSDPLFGGEPVDFHDLTAALTKVRSGLDGQISQMSHLREQNGQLEKELSALEV
                     RLKEVERLKYELELVRKAREAQEAQLEKVKYEVLTVGEEIDRITKALEDKFGNSLVVT
                     QHQDRLVLTMLGQVLFESGEADLTPLGLKIMKQVGQVLATVPGKNIQVEGHTDNHRIY
                     GQLQKQYPTNWELSTARATTVLRYLIEQTGMDPKQFSAMGYADMRPVMTNETEEGRAQ
                     NRRVEIVLYPERVIQGNETVATLGP"
     gene            complement(17522..19114)
                     /gene="nhaB"
                     /locus_tag="VLA60_05850"
     CDS             complement(17522..19114)
                     /gene="nhaB"
                     /locus_tag="VLA60_05850"
                     /inference="COORDINATES: protein motif:HMM:NF007093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HSF08917.1"
                     /translation="MKTTVPQALIQNFLNHTPTWYKLTILGFLILNPILLMTIGSFYT
                     GWILILEFIFTLALALKSYPLQPGGLLALEAVLLGMTTPATVYHEALNNFQVILLLIF
                     MVAGIYFMKDLLLFLFTKILLGVRSKILLGLLFSIMGAFLSAFLDALTVTAVIIAVAL
                     GFYNIYHRVASGKSSQTSHDSTSDHEIGSLHRENLDNFRGFLRNLLMHGAVGTALGGV
                     CTLVGEPQNLLIADKAGWEFMEFLLRMAPVTLPVLATGLFTCMIVEKMRWFDYGFQLP
                     DPVRLILLDFETEQQKRRDSGDTAKLIIQAIAGIWLIFALAFHLAEVGIIGLTVIVFI
                     TAFNGIIEEHQLGEAFKEALPFTALLVVFFAIVAVIQDQNLFSGIIGYGLSLQGSHQI
                     GMFYLANGVLSAISDNVFVATVYIQEVLRAFQAGTITRDQFDLLAVAINTGTNIPSVA
                     TPNGQAAFLFLLTSSLAPVIRLSYGKMVWMALPYTLTMASVGLFSVYFLLDPATAYLY
                     ETGLLQHHSLSSPGTLTEPLAH"
     gene            complement(19314..>19860)
                     /locus_tag="VLA60_05855"
     CDS             complement(19314..>19860)
                     /locus_tag="VLA60_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF08918.1"
                     /translation="TAVGIGVVLASVMFVKRMADLELENLSVITGASKETPLSKEEQR
                     ILDRNRERIIMIHVNGPMSFGSAKDMVRRLESVKSFNTFSSVVLDLTSVPSIDGTAAL
                     AIEDMLLMVKSHQQHVFFVGMRPGVARVLKGLGVLDQVPPEHRYPLRLDALRHAAYIG
                     GSTHPYDVAPTEIQSELPEPS"
BASE COUNT         4538 a         4543 c         5349 g         5430 t
ORIGIN      
        1 atcgggtcga gtgccttcgt cccgtgcggc cattccaaaa tgatgatttc caaaacatgt
       61 attttcgcgg agatacggtt gagctccggc aaacgaaaaa atgccggact cacgattatg
      121 agagatctcg ttgtcgagca aggtcgggcg gcaatccggt ccataaataa cgactccgga
      181 taaaagccct tctgtggccc tgcagtgaga tatggtacac accgaatcta aaatattcag
      241 ggcggaatgt tggtcgctgc ccacataccg aaatgtgata ccactgatct gtccgccgga
      301 aactcgttga agatagcaag gaccgctccg ccgattgaat attgtgacct ggtcccgacc
      361 ggcgccgaca agatggatgg ttcgctcagt cattaccaac cgatcctcat aaacccccgg
      421 tcgaatgaat attagatcat cttgttgcgc gtgagcgaga gcttcgctgg gtagggtgaa
      481 gcaatctggg tgctccgcat ccacaattaa cgtttttccc ccaaaaggat tgggtggcgg
      541 ttcgtgaaga ggtgattgtt cagcagacaa ggccatctcc catttcaaaa tggttctgtc
      601 aaacgtgggc catgaaatct acggtgaaaa tgtattgaac gccctcaagc agacgaatac
      661 aaaccgaagg gtgaaccata caggattttc ttagattgac atttcattat ccaatatcca
      721 atccccaata taagggaaaa atgcctgtaa tattcccatt tcctcttctg gtattgaaat
      781 tgctcgggct cttcccttgt acaaaaaata aattctaaag tcttttttgt ctaatttttt
      841 ttaatgatcg aagaattttt ctgcatgagg agagaagtct gattccccat cagtaatttc
      901 aactgctttt gtcagaaaag tctgactccc actccgtaat cccaactatt ttcccccagg
      961 aaagcgagct ttctaatgcg acggtatgag aggagtggta tgtccatgtt ctttttgttc
     1021 atagtgctga gcacgatgtc gtttgtaaca tgtttacctg gagcgcaggt acaggaaacg
     1081 gatatttttt aacatcctaa tactgaacag gaagggatta agaaatcttt agagcagcaa
     1141 attggggctg gccggggaga tattaacact cccgattcct cgctgtacat cattagccgg
     1201 gatccgttcc gctccattcg tcgtggtcga aatctttttc aacgaaaatt caccgtatcc
     1261 caaggaatgg gcccacgtaa tggtgacggg tttggagata ttagcgccga tgccacgctg
     1321 ggcgcagggt tagcagatag ttgcgcggcc tgccacggac gtccacgagg atcggcggga
     1381 tttggttgag tcgtgtttac ccgtccagat agccgggatg ccccgcattt gttcgggtta
     1441 gggatcatcg aaatgcttgc ggatgaaatt tctcaagacc tccggcaaac ccgggatcag
     1501 gccattgcca aagccaaagg gagacgacaa tcggtggagg tctcgttaaa gagtaagggg
     1561 atttcctatg gaaacattag agtgaatcct gacggttcgg tcgatccatc ccgattagaa
     1621 gggggtgatg aggatctcgg gtacgcccat tttttcatca tggcggcacc atttccatca
     1681 gggaatttgt tgtcggggca ttggatggag aaatgggtat ggaggctttg atccggattt
     1741 ggccaaggcg gctaagggtg gagaggtggt aacgccatcg ggaatggtgt tgaatggaaa
     1801 gattgatacc atcgaagcac ctccggctct ttgtgaaagt gatgatccgg atcgggatgg
     1861 ggtggccaat gaaattcccg tgggtttggt ggaccacctg gaattctatt tgttgaatta
     1921 ttttaagcca ggcataggcc gccagaccag agatactgaa caagggcaga aactctttca
     1981 ggatattcaa tgcaccggtt gccatatcca aaatttagtc attgagaaag atcgacgggc
     2041 ggctgatgtg gaaacttcat ttgacagccg ccgagggatt ttcaatgacc tttttgccgt
     2101 ggcgactcct ttgttccaag aggttcctgg ttcaggttct ccatcggtga aacaacccaa
     2161 gggtcagcgg tttgtcgtga agaatattta tgcggatttt aaacggcata atcttggccc
     2221 caacttctgg gaacgcaatt gggatggtac cttgaccaaa gagttgatga ccgaaccgct
     2281 atggggagtg gggacgactg ccccttatgg gcatgacggg aggagtattg atctgtggtc
     2341 agttattatg cggcacggtg gtgaagcgca aaatgctcgc gatcgctttg cccgattacc
     2401 gaaagtgaag caggggcaaa taatagattt tctccagacg ttggtgttgt ttccacctga
     2461 cgatacggct tccaatttga atcctggtga ttcgcaatca cataatttcc ctcagtacga
     2521 acatggcaat attgcgttgc cttcattatt caacgatcca agcgatttag aataaaggga
     2581 cttttcctcg ttctttcctg cctcttggta tcaaacccgt gagctctaag ctcacgggtt
     2641 tgatccctct cttgcaatga gagatgtccc gccattgcct ttttaatttt tgtgcggtgg
     2701 aacaagcagg aggaacaaac tacatggttt tcaaattctt tgcttctcct tccccttgct
     2761 tttcgcctca tcactcattg ttaggcgccc caaaaccatg ttgagggtct attttgagct
     2821 atatcggccg gcagcaatca caataagtat tgggggggaa tcgggagaag actttgcttg
     2881 ccgacaagta tgtactgagg tatggcaatg agcgagttaa ttgttctcaa atgcttgacg
     2941 ggttgcgaag acctcgcctg gatgaatcag gagtagacaa tcgccatcaa ccggatcctt
     3001 gtgaaatagt gcaaaggtga ggcagagctg aggggtgggt tcaggaggaa atggtaacaa
     3061 aataggaaca tccaccagcg gagttggtgt tttcaggatg gccaacctga ggcggacagc
     3121 cacgagtgta tcgcgtagac gggtttgaat aagttcttcg gtataatccg aggaaggagt
     3181 gatgtattga tcccagagcg ggttggtcat gcccacgcaa aaatccagcc ccacgctttg
     3241 tgcgattggt gtgacatctt tgagtattcc ggattcaatg gcttcttttc gggatcggat
     3301 cgggagtgat tcaaattcag caggaatatc cccagaactg agtgaaagac ctgattggga
     3361 cggttcagag aaatttgaag gcaaaagatc ggatctttcc ggagactggt tgtgtacttt
     3421 ttcggaagga ttaagatcgt tcaaaggtgt cttccctaaa gcttgaagaa ggccttcata
     3481 gacagctttg gcagaatccg tccacgaatc attcatcggc aatccccgaa aggccgcctc
     3541 agaagctttt ttctcatcat gggttaaaac acgttgctct ttcatacttt caaagataag
     3601 gcaaatcccc tggaagtcaa gagtaaattt tgttgtccat ccaaaaagcc gggaaatgga
     3661 gaacaatgaa aatagaaaac cagtatatga gaattctcag acggtctaac cgccccatat
     3721 ttttacaggg tatctgtgag ggggttgatg gctcaaaatg aggctgttta ccggaatatc
     3781 gcttccaaat cacgatctaa cgtcaggacg atgatgcgtt cttcgatccg ggtatcgata
     3841 tccgtaaaca gggcagttgt ttccgcaccg gtgatctcca ggatttcacg acacagttgc
     3901 tctcttcctt gggagatgag attttggcgc atctgtttga ccattccaat gccttcttca
     3961 ctcttagcca gctggcgttc ggctggcgta agaacgcctt tgagccgaat gaggatagtg
     4021 tcgcgtagaa tgctggctcg aacatcggtg gggccgcggc ccatgaattc ctgttcaaat
     4081 ttaatgatgg catttcggat cgccgattcc atttcacctc gtgtgaagag gggtttcata
     4141 gcgtactgct ttccggcaag acggaccttg cccattcact atcgatctat cacaatcccg
     4201 taggaaagaa tctcaggaac ttgaggagta acctatctcc ttgatataag cgtattgttc
     4261 atgtgaggtc aagaagagaa tgggcttctc ctcgaatggc tgaggagggt ggcaactgtg
     4321 ctggttttcc ccttggtcgt agaccgggtt tgtcggatcg cctgcatcat tcaccatgtg
     4381 attgaggtac gcgatagaat aagccaaccg agtgatctcc tgaagctaaa aagaggagat
     4441 gcccatcggt tggctttttt aattgtacat agagtttatt gcacgctgct aattcactta
     4501 aatctaatga gttaggtgta tgatcttgtc aggctttccc ctctgccatt tcaccctgat
     4561 tcccgttatt ttaatcagta agagcacaaa tgatttcttt aaaccttgat ttgacgctat
     4621 gagatgtccg tgctcttaaa aaggggaaat ggggcaatga acaggcttga gcaagatcgt
     4681 tcaacaatga cgaattggaa atggctgcga gaaatcgttg atgaggcgtg tcagcccatc
     4741 gggccatatt ggcccatgaa aacctttgcc tattacaatc ctattcggga cctcgagcat
     4801 ttaccctttg tccgtgcgat tgaggaagcg aatcagttgc tgggcgccaa gggatttctg
     4861 cctgttgaag agtatcgcca aatcttttat cagggtcgga ttactctgcc ggctcttgaa
     4921 cgggcacttc ggcgggtggg gcccccatta cctgcacggg ctaccattag agttggagca
     4981 cgcaccatac acgtccacga tgtctggcgc attcatgccg catacgggat tgaaatctta
     5041 ccggctgtgc tgatgacgtg gaccctggaa ttagagggtc tgaccacacg ctttcgatcc
     5101 gatcttccgg atgattctcg ctcctccatt attgatcgaa cgattcggga gtgcgaaaag
     5161 tgccgccatg atccggaatc cgcctatctg cataacctct ggaaaagttc gttggcggcc
     5221 tgccagttgt ctgaccctgt gtcgggtctg ccttctttcc acgcaccgga tcccggttgg
     5281 agcgctaaag catcaggata taaagttgga tataaagttg cactcgtccc ggttgattta
     5341 cctatggaca ggacgctggg tgattggctg gacagtgaga cgggtagcgt gctggttgag
     5401 actatcaata cgcacatgat caaatggatt ggggcatttg tcgatgaagg tgttgcggga
     5461 tggagtatgc cttcaagaga caaggggttt tatggtgcat ggcgagaatt agccgaaggg
     5521 gatctttcag gcagattcct cgggattcca gatcttcgac aaaaattcgg tgaactgtct
     5581 gaagtaccgg aggaaatgtt gtgcaaacat ctggaagatc tcaaaattcc aaaggaacgt
     5641 tggcaaaact atctctcacg gcatctggct caattgccag gttgggcagg gtttatccga
     5701 tggcgtagtg accacaccgg gtattctgct caacaacact atcccattga tccactgcaa
     5761 tatctggccg ttcgcctttt ttatgaatct ggaatggtag agggactttg tcaacgggaa
     5821 tggggcatca aggggacgtt acctgcactc ctcgcttatt ggaacgaact acgtgagcgt
     5881 gaacaggcgt tgagcctccc tttttctcat gccaccgacc ctaatcatca tgcggtgtgt
     5941 catcaagctt ggcgactttt tcacctggct cagtttttgg aattgacccc aattgaggtc
     6001 catgatttgt cttacaccga catgtcaacc ttactggagt ggctggacct cttcccccaa
     6061 tctgcccatg gaccggtatg gcttgaagcg tatgaggacg tttatcggga gaatttgctg
     6121 agaaacatca ggggacatca aggggtggca cccgttaacc aggagcgacc acgggcacaa
     6181 gggatttttt gtatcgatgc tcgctcggag tcgtttcggc gtcatcttga agctcagggg
     6241 ccgtatgaaa catttgggta tgcgggattt tttggcgtgc ccatgagtca tgtggcattt
     6301 gatagccacg accacttagc tttgtgtccg attttgttga caccaaatgc ggaagtgacc
     6361 gaagttccgc gagtcgggca gaatgatcgg gtgaaggact atctctcggg aaccaggtgg
     6421 catcaactca gtcatcatct cttccatgat ctgaaacaca acccatttgc gtcttttatg
     6481 ttgattgatg tgctagggat gttttttagc gtcgggttgg taggaaaaac actgtttcga
     6541 acctccttcg atgccgtcaa gcagtggttg cagcagtggt tggggggaac cgttgtcacg
     6601 caaattcctg ttgaggcttc acatgaaaac gagcagggaa atccacaatt gggaggactg
     6661 gcgctcggtt tcactccatt tgagcaagcg gcctttgtgg aggggggatt gcgagtgatc
     6721 ggcctcacga aaaactttgg ccggtttgtg atgatctgcg gtcatggaag ccagtccgaa
     6781 aacaatcctt attatgcggc gctggattgt ggcgcatgtg gaggcagtca tggcgacccg
     6841 aatgctcgtg tctttgctgc catggccaat aatccggaaa ttcggaaaat tctgagcgat
     6901 catggtctgg tcatcccaga ggacacctgg tttcttcccg ccaaacacaa cacgaccaca
     6961 gatcgagtga cgatgtatga cctggtcgat gttcctgcta cccatgtaga ggatttagca
     7021 ctgttggtga gggacttgga gcaggctggc acacatcagg cattggaacg atgccagcgc
     7081 attccagggg ctcccaccac ggtgtctccc cgtgacgcgt tcaagcatgt caggcagcgg
     7141 agcatggatt gggctaattc gcgtcctgaa tggggattat cagggaatgc cgcattttta
     7201 attggaaggc gggcacttac caaaggtctg gatttggacg ggagagtctt tttgcattct
     7261 tacgaccccg gcccggatct tgatgggagt cttcttgaaa aaatcatgac cgcacccctc
     7321 atcgtgggcg aattgattaa cttaacgtat tacttttcgt cggtggatcc ttgggcctat
     7381 gggggtggga gtaaggtcat tcacaatatg gttgccggag ttggtgtgat gttagggagt
     7441 caaagtgacc tgcaaaaagg ccttcccctc caatcggtaa acgatggggc gcggcactat
     7501 catgagccca tgcgcttact ggccatcatt gaggcgccgc cggatcgaat acggtctatc
     7561 attcagaagc atgctttgct tcagcatgtt ttccataacc aatggatgaa tgtcatcgcg
     7621 tttgatccgg aatccaaaag catttcacgg tatctctctg acttaacctg ggaaccagcc
     7681 acgatgaaca tatgatttca cgaaaaaaaa tgaacgcctt ctttggcttc tgccgtgctt
     7741 tgaattcctg atcgaaactc ctatcatctc caacgcggga tgattattcc ttttgattcg
     7801 aagttgtgga tggaagaggg tgcctggtat aaggtcggag gcttgcagat tgttcaaaag
     7861 gatgaggccc ctttttttat ttggcgcaag ctgagtcgga gagtgggagc atccggtttg
     7921 ccaaaatatc atttatcatg gcagtagggt tcccggcatt tggttagttc cgattggctg
     7981 gagacagtac aagccaacca ggttttttga acacctcatt cgaaaaacat ttggttggct
     8041 tttttttata ctttatcggg ttccttgagt cgagccgttc agggtttgat ctgaagatca
     8101 tataggatga aatcttcttt ccccaaacaa tttatcgcaa acaccatggc cctattttgt
     8161 ggctggcttg tgttatcggg gaagtacgac aattttcacc tcgttctcgg attcacggcc
     8221 tcttgcggag tcgcatggtt aaacacgggg ttccctcatt ctccctttca gcaattcccg
     8281 tggggccgta tggtgctgta cagcccctgg ctttttttac ggattgtgga aagtagtctg
     8341 catttgacga aactcattct gaatccctcg ttacccatta agcccagact tatcacctat
     8401 cggtctcaat taaaacatcg gggtgcgatc gttgtgcttg gaaattctgt gaccctaacg
     8461 ccaggcacca ttaccgttga aattaacggc aatactttcc tcgtgcatgc catagatgag
     8521 gcagcaggga atgatctcac caccggccgg atggagcgca aacttgcctg ggtctttcag
     8581 gaagacacga atttgtgatg attgattttt ttctattttt attacttacg ttatcatttc
     8641 tgatcctcgt gtatttatac cgggtgatcc agggtcccac ggtgtttgat cgggtattag
     8701 gattggcggg aatttcgacc aaagccatca ttctgggcgt cgtcattgga acagtctatg
     8761 accgtgtgga gatgtttgtg gacatctcca ccgggtatgc gcttctgaac ctggtgggag
     8821 cgtttgccat tgccaaattt cttgagcaac gaggggcacc ctaaatggtt gttattgcca
     8881 tcgggttaat tctggtgggg gtgtttttcc tggtggtggc tgcaattggc acactacgcc
     8941 tccctgatgt gttttcgcga tcccatgcgg tctccctgac tgattcccta ggcgcgattt
     9001 gtgttctcgc gggccttgct ttgtaccagg gtttcgggat caatatgctg aagattctgg
     9061 tcgtcctggt cttgctcatc ctgttaaatc cggttattgc tcatgccacg attcgagcgg
     9121 cctatcggtc tggcctcaaa cctcagacgg aggaagacca atgatgtatt ccgtgaatat
     9181 tgccttgctg ttacttctgg tcctgacggc ggccagtgcc acatttttgg ttaaggaatt
     9241 gatgagttca gtgtttatcc tcgggtcgta tagtttcttt ctgtctctgg tttgggcttg
     9301 gttaggtgcg gtggatgtgg catttgtcga agccgttatt ggtgccgggt tgggtaccgt
     9361 gttgtttctt gtcacgttgt ttgataccgc tcccaaggat agccgccttc gtcgtccccc
     9421 tccacctcta gcgggagtcc tcggactgcc cttgctgggc ctgctgttgt tattagcggc
     9481 gaatgatctt cctcagtttg gcgacccaac ctctccgccc aacgtgcaca tttctcctgt
     9541 gtatttacag aatagctatg cggacacgct tacgccgaat gtggtcacct cggtgctgat
     9601 ggactatcgg gcatttgata cgttgataga aaccgtggtg atatttacgg ccggcattgc
     9661 ctgtgccttg ttattacggg agccaggctc atgaaacaga tgcacgacag catcattgtt
     9721 caggccatga gtcgcttgct cattcctctg gcccagttgt ttgcgtttta cgtcttgttt
     9781 ttcggccagt acggtcctgg aggaggattt gtggccggcg tcatttggac aaccagtatg
     9841 atcctgactt ttttggtgtt cgggctgaag tcaccccagg gacgtttggt ggaaaaggtc
     9901 ttgcatggcg atgggatcgg attgattatt tttgtgggag ttggtggctt atgcctcatc
     9961 ggaggtgggg aatttctgaa ctacgccaat ttggagattc cgggtttgga tgctcccgcc
    10021 cgccggtata cggggatttt gctggcacaa atcggcgtgg ccgtggatgt tgcggtgaca
    10081 gggatttcta tcgttctcag cttggcctat cacgataccg agcaggaata tgatgtttga
    10141 gatgatcggg gggtggctgc agcgccccaa ctacattgct tttgtcctgc tgtttctgtg
    10201 gggtatctat attatggtga cccggtacaa tctggtgaaa aaactcatcg ggatgtatct
    10261 catgcaaacg agcgtgatct ttttcctcgt caccacaagt gccaagcaag gcgccacggt
    10321 gcccattttg ctctccacga ccgagatcat tcaaccggaa gtctatgtga atcctctccc
    10381 tcatgtgttg acattgacgg cgattgtggt gggtgttgcc accctgggag tatctttggc
    10441 gctggccgat gccatttata aaaaatacgg cagcctggac gaagaagaaa ttctcaaaag
    10501 gctggaatga cgcgacacct tcccgcaatt cttttcctct tacctctcgg tttggccatt
    10561 tgtttaccat taatcgggat caagcatcgg gagtggtgtc ggccattatc gattggtgtg
    10621 ctggctgcga tggtcccggt ttccttcgct aatctggtag gagttcttca aaacgggacc
    10681 attcattatg actttggggg atgggccgct ccggtcggga ttgagtgggt ggcggacggc
    10741 ttggccagcg tcatgaccgt ggctttgagt ctcgttggat tggtgtgtat caccttcctc
    10801 agttcagttt tgtttcctta ccttggcagc cggattgtcc cgttttatac cctggtctta
    10861 ttgttgatag ccagtctgac cggcatggtg tttgcggggg atttgttcaa tcttttcgtt
    10921 ttcttagaag tatccgccct gtgtgcctat gccttggtcg ggttggctgg tgggaaggcg
    10981 cttgtcgccg gattccggta cttaatttta ggtaccttcg gagcctcctt atatctcctg
    11041 ggagtggggt atttgtacgc cgagacgggt acgctcaata tggcagatct ggctcagcga
    11101 gtgccctctt tggtggcatc caaggccttt gcggttggtg tgttattcat gttcattggc
    11161 ctgggcatta agatggcctt ggtaccgctc cacgggtggt tgccggatgc gtatacgtat
    11221 gcgccagata gagtgtcacc gatgctggcc tccttagtga cgaaggtcgc gctgtatgga
    11281 tggattcgca ttatgttttg ggtccttgga gcccaggcgg tggtctacca gatccctatc
    11341 ctgtttttag tgggagtctt gggcgctctt gcagccctgg ttggcgcatt cctggctctg
    11401 tcccaagtag aaatcaaacg catgtttgcg tatggagggc tttcgcatat cggattgatt
    11461 ctggtgggga ttagtctcgg aaatcaaacc ggatttgtcg gaggagtgtt ctaccttttg
    11521 aatgatgccg tgatgcaagc gggattgttt ttttgggcgg gggcaattat tcatctgtac
    11581 ggggttcgca ctattgagga tcttgggcgg ctgcagggag gagcgggatg gatgtcggtg
    11641 gctctggtcg ttatagctct gtcgatgatc gggattcctc caaccggggg attttttgga
    11701 aaatggtata tcattcttgg cgcggtggaa gcgcaaaatt accttgccat tgtggcggtg
    11761 ctgattgcga ctctgttaac tctggcctat ttcgtgaaat tatttgaacg gatatttcgg
    11821 gaccggtcca ccctgcctcc tcccattata cgggagattc cgttggcggt gaaaattggt
    11881 ctgggcgcca cgtcagtggc ggtcattgtt ttgggactga tgagcgatca cattgtcagc
    11941 tttctcctca agaacgcggt accacccggg ctttagcagg attgggcata tggtgacctc
    12001 ctgattatga cattcctcat ccttattcca ctccttcctc tgatggccgt tctggttctc
    12061 gtgctgggga aaccctggct tggagagcaa ggccataaga tcggcattcc tgcgatggct
    12121 ctctccttcg ctttttccgc catcgcgttt gctcgagttg catcagacgg ggccttctcc
    12181 attccactct accgactatt caaatctgga gacctggttg tagatttggg tctctatgtc
    12241 gaccagctta cggtgttgct tctgctcttg gtgactggtg tgagcagcgt ggtgcatgtg
    12301 tatgcgtcac gttatatgat cggcgatccc cggtataacc gtttctttgc ggtcatcgcg
    12361 ctgtttacgt tttcgatgat catgctggtt atgagcaata atctgctcat gctgtttgtg
    12421 ttctgggaga tcatggggat ctgttcttat ttgttaattt ctcattgggg ggagcggcaa
    12481 tcatctgctc aggccgcgac caaggccttt ctcgtgaatt cggtcgcgga tgtgggctta
    12541 ggttttgggg tcattctgac ctattccact tttggaacgc tggatattca gacgatttta
    12601 ggggaagccc catccataca gggaaacacc atcaacctgt tcggctggct agggctggac
    12661 gtgcccattc atatcctcac gctcattacg ctttttcttt tcctgggagc catgggaaag
    12721 tccgcacaag tgccgttcca tgtctggctc ccctttgcca tggaagcgcc cacacccgtt
    12781 tccgcgttga ttcacgccgc gaccatggtg aatgccggac ccttcttgtt ggttcgcatg
    12841 agtcccctcg tgatcctttc gcccgaagcc atgaccctca ttgcattggt cggaggcgtg
    12901 accgcacttt ttgcaaccct tgtctccctg actcaggtgg atattaagcg catgctggcg
    12961 ttttccacca ttggtcaaat cggtttcatg atcatgactt gcggagtcgg agcctttgtc
    13021 atctctattt ttcatatgtt ggcccatgga tgtttgaaag gatttttatt tctgtcgact
    13081 ggagatgcac ttcgttccgt tggctcccag gaacatgtgt cttcgtcgca tgttcctcat
    13141 ggctcatcaa tgcgttctat ggggccctta gtgttaggag ctttgctgtt ggcctgtctt
    13201 cccccgttcc tcattttttt cgggccgtat gagcaattgt ggatgattca accgggatcc
    13261 tccgcccaat ggacattttg gattttggga tttctgacgg tatttttctc tgccatgtat
    13321 gtcatgcggg gtgtcctgct cttgtttggt caaccgtatt cgataggtca tggaagtgga
    13381 atggggaggc aggccatcca gcctcgtttt ttttcccctc tccaccttct gggaattttg
    13441 gctgtcatgg gaattttggg aagcctgttg ctggtcttgt ggagttggtt ttcggaattt
    13501 ctgtcccctg tcgtgggaaa tcggatggcg cagattccaa gggatacttc gagtgagacc
    13561 ggcttgcaac ttatttttgc atttgctgtg gctgtgggcg gtatcgcggt tgggtacttt
    13621 tccttgacaa aattttcggg ttcctggttc caacaatcgg aaatgagcaa gcgatggtat
    13681 gtccactttc tgaataaatt gtatttcgac gaaatatatg aggcgtatgt tgtggtgccc
    13741 actctccggt tggcccgatg gctatggcgg gttattgacc ggaatgtgtt tgacgccctg
    13801 atcatgggaa tcgcaaatgt gtcagtgttg gcggcgaagt ggatgtggcg ggtggtggat
    13861 cttcggggga ttgacgggct ggtagtaggt ttgggacgga acagcgtagg aatggccggt
    13921 tggctctggc gggtggtgga ccttcgcggt gtcgatcggg tcgtcgtggg tatcggccaa
    13981 cgcagcattg gtatcgccaa ctggctatgg aaaagaatcg agatcaaagt tctcgacaag
    14041 aatgtggttc gtgccgggaa tcaagccgcg agcacaggaa atatgatgca ggaactagag
    14101 cctcgcacgt tacagcacca cttactggtc atgattttct ggctcattgt ggggattggg
    14161 ttgttgtttt ggtttgttgt gtgattccgc agatagcttg tcagtgattc agagggcacg
    14221 aacacgacac ggcaattcaa actcgcggtg tgtgaaaaat gagagggcgt ctttcatgtc
    14281 ctggatgtgc tatgaccggc atgtcatgaa ggggagacta tgaacacaat cttcactgaa
    14341 catattttga gtatcatgat cgcgatgccg tttttgggca ttgggatcat cgcgtgcatt
    14401 caggatcagg aatggattcg gcgcgtggct ctggggtgta cgctgggtga cgttggtctt
    14461 gcactggtgc tgctgaaaga atttgatttt tccactcaag gcatgcaatt cgtggagcgg
    14521 atggcatgga tgccgacctt taatatccaa tatgctgtcg gagtggatgg cattagtctt
    14581 cttctggttt tactcacgac tctgctctgc cccttttgcg tcctgtgctc ctggaagtca
    14641 attgccactc gtgtacgctc ctttatggcc atgattctgt tggtcgaagg ggccatgatt
    14701 gtggtgttta ccgctttaga cctgtttttg tttttcatgt tgtgggaagt cacgatgatt
    14761 cccatgtatt ttatgatcat cctgtggggc gggccccaac gcatagcggc cggcatcaaa
    14821 ttcgttctgt acagtttgtc aggcagcctg atcttacttt tggcgatact cgggttgtac
    14881 gtggaagggg ggcggacatt cgatattttg gcgttggcgg agaacaccta ttcccccgac
    14941 gcccagtttt ggatatttct ggcgttcttt ctggcctttg ccattaaatt acccatgctg
    15001 ccgtttcaca catggctacc ggatgcccac tccgaagctc ccaccgccgg gagcgtcctg
    15061 ctggctgggg tactcttgaa aatgggtgga tatggatttt tacgattttg tctccccatg
    15121 tttcctgaag cctcagtgac ctttgccccg tttatcttgt ggctatccgt ggcggctatt
    15181 gtgtatgggg ggtatatggc cctggcccag tcggacctga aaaagctggt ggcctattcg
    15241 tccatttcgc atatgggatt cgtgacattg ggcattttcg tgtttaataa ccatggaatt
    15301 caaggagcca tcctgcagat ggtgaaccat gggataacaa caggagccct atttttggct
    15361 gtcgggcaat tgtatgatcg gacccatagc cggtcgattc aggattacgg agggttgcaa
    15421 aaatcgatgc cccggtttgt ggcacttttt tgtctgtttt cagtggcatc tttcggactg
    15481 cctggcacgt gcaattttat tggagaattt ctggtcctgg tcggaacgtc ttttgaaagc
    15541 tatgtcatgg tgttgttgtc catggggggc attgtcctcg cggcggcgta tatgctctgg
    15601 atgcttcaac gggtggtgct gggtcaacca aaaaattcga ccattgcaca actaccggac
    15661 ctgaatatgc gagagttggt cacggtgatt cctcttgcgg ttttaatctt ggggatcgga
    15721 ttgtacccgt caccacttat ggagatgatg gaatcgagcg tcacgcacct ggctcagcat
    15781 atggcacagt atcaggtggg gatgatccag gaggtgtccc ttcggtgaag gaccaagccg
    15841 gcaattgcga caaggacgat agtttccgat gaaccatgac ttggacatct ctcggttggt
    15901 gagcacgaga gataacgatc aaacttctca cattcccact acccttccgc gccaacattt
    15961 tctcccttca tctttccgca ttgattccct gtgaattgaa acagtgtcgg tggtgctcat
    16021 tgggaaatat tgacaggtca ttctgacctc ttggagcaac ttcctccttg tttctgcagg
    16081 ttttctcaag tgcccgatcc agaatgccga gaataatctt cactgctctt tctggtggcg
    16141 aatgaacctt ccggtcacta aggttggcca tggcacccag aagagtagat tgaaaccata
    16201 taggccaaaa gttaccaacc agtcttgaat gctcaggtgt cattgatagt agagaacctg
    16261 gggtgaggag aggcggtaaa cgcgaaggag caggcactat gaaactgggg aacactttac
    16321 ttgcggcagt ggcatgtacg attgtggtcg gttgtgtgag tcagcaatca catgaacgtg
    16381 ctctccaaga atacgaaaaa aaccaagagt ccacgatagc ggaacttgaa cgcttgcagc
    16441 aggctgttga agagaagcaa gcagaagtcc tgcagcttca agaaactaag acggcccttg
    16501 agcataccgt tgaagtgcaa gatcaaatta aggaagaaca gttagccgcg gtccaacaag
    16561 aagtcgaagc gctgtatacg caaatccgga aactcgtcaa ctcggaagga accgttactc
    16621 tgtccgatcc tctttttgga ggagagccgg tggattttca tgatttgaca gccgcgctca
    16681 caaaggttag gtcaggttta gacgggcaaa tcagccagat gtcccacctt cgtgaacaga
    16741 atggtcagtt ggaaaaagag ctttcggccc tggaggtcag gctcaaagaa gtggagcgtt
    16801 taaaatatga gttggaattg gtccgaaaag cccgggaagc tcaggaagcg caactggaga
    16861 aggtcaaata tgaagtgctc acagtcggag aggagattga ccgtattacc aaggcgctgg
    16921 aggacaagtt tggcaacagt ctagtggtga cccaacacca ggatcgatta gtcctgacca
    16981 tgctgggaca agtcctgttt gaatccggtg aagcagattt gacccctctg gggctcaaga
    17041 tcatgaagca ggtgggccag gtgttagcaa cggtcccagg gaaaaatatt caagtggaag
    17101 gccacacgga caaccaccgc atatatggac aactacaaaa acagtatccc accaattggg
    17161 aactttcaac cgcacgcgcc acgacagtgt tacggtatct gattgagcaa accggaatgg
    17221 atccaaaaca attttcagcg atgggctatg ccgatatgcg accggtgatg acgaatgaga
    17281 ccgaggaggg aagagcccaa aatcgtcgtg tggaaattgt cctctatccg gaacgagtga
    17341 ttcagggcaa tgaaaccgtt gcgacacttg gcccgtaata ggaggcaagg gccataacaa
    17401 acaatctggc ttcagaagtg gttcccccct ttctattccc ctcttgcatc gagccatggg
    17461 tcaaccttgc tgcagaggtg acggttcttt tccttcttcc agtatctgac ttctccaatc
    17521 attaatgcgc cagcggttcg gtgagagtgc ccgggctact taagctatgg tgttggagga
    17581 gtccggtctc gtataaatat gctgtcgccg gatccaacaa aaaatatacg gaaaacaggc
    17641 cgacacttgc catggtcaac gtatagggaa gtgccatcca taccattttc ccataggata
    17701 agcggataac cggagccagt gaggaggtta ataaaaataa aaatgcggct tgtccgttgg
    17761 gggttgccac actggggata ttcgtgccgg tattaatggc aacggccaaa aggtcaaatt
    17821 ggtcgcgggt aatggtgccc gcctggaagg cacggagcac ttcctgaatg taaaccgtcg
    17881 caacaaagac gttgtcgctg atagccgaaa gaacaccatt ggcaagataa aacatgccga
    17941 tttgatgaga tccttgcaaa cttaatccat acccgatgat gccggaaaac agattctgat
    18001 cctggatgac cgccacaatg gcgaaaaaga cgacgaggag agccgtgaaa ggaagcgctt
    18061 ccttaaacgc ctctccaagt tgatgctctt cgataatccc gttaaaggcg gtaataaata
    18121 cgatcaccgt gagtccgatg attcccacct ccgccaggtg aaaggctaag gcaaaaatca
    18181 gccaaatgcc ggcaatcgct tggataataa gcttggccgt gtccccggaa tcccgtcgtt
    18241 tctgttgttc agtttcgaaa tccaacaaaa tgaggcgaac gggatccggg agttgaaagc
    18301 cataatcaaa ccagcgcatt ttttcaacga tcatacaggt gaacaacccc gtggctaaga
    18361 ccggaagggt cacaggggcc atacgcaata agaactccat aaattcccag cctgccttgt
    18421 cggcgatcaa taaattttgg ggttccccca cgagagtaca gactccgccc aatgctgttc
    18481 ccaccgctcc atgcattaat aaatttcgaa ggaagcctcg gaagttgtct aagttttccc
    18541 ggtgaagcga gccgatctca tgatcgcttg tcgaatcatg agaggtttga gacgatttgc
    18601 cagaggccac ccggtggtaa atgttataaa aacccaaggc gacggcaatg atgaccgccg
    18661 tgacggtcaa ggcatcgaga aatgccgaaa ggaaggcacc catgatggaa aacaagagac
    18721 ccaacaaaat ttttgaacgg acgcccaata aaattttcgt aaacagaaac aagagcaaat
    18781 ctttcatgaa gtagatcccg gccaccatga aaatcaataa caggatcact tggaaattat
    18841 tgagcgcttc atgatagacc gtagcgggtg tagtcattcc caataggacg gcttcaagtg
    18901 cgagcaagcc accgggttgt agcggataac ttttgagagc cagtgccagc gtaaaaataa
    18961 attccaggat gaggatccat ccggtgtaaa acgacccaat ggtcatcaac aagatgggat
    19021 tgaggattaa gaatcccaga atggtcagct tgtaccaggt tggagtgtgg ttcaggaaat
    19081 tctgtatgag cgcttgaggg actgttgtct tcatttcagt tgggtaacct ttcggaccgg
    19141 tggtcccaga tagagtctga cgattaaacc aatatcacta gggtcaatac actgattggt
    19201 gatttctctc gcatccaggc ctataaaggg gtgagggcag ggcggagttc ctcagcttac
    19261 actatgaatg ctttttcaga aacagagaag gctatgtcat caaaaaacat acctcatgaa
    19321 ggttccggga gttcagattg aatttctgtg ggagcgacat cgtacggatg ggtggatccg
    19381 cctatgtaag cagcgtggcg tagggcatcc aaccgaagag ggtatcgatg ctcaggtggg
    19441 acctgatcga gtaccccgag tcctttgaga actcgagcga ctcccggacg catacccacg
    19501 aaaaagacat gctgctgatg acttttcacc atcagcagca tgtcctcaat ggctaacgca
    19561 gcggttccgt cgatggaagg caccgatgtg agatcgagaa ccacgctgga aaaggtattg
    19621 aaacttttca ccgattctaa gcgacgaacc atgtctttag ccgatccgaa gctcatcggc
    19681 ccattcacat gaatcatgat aatgcgctcg cggtttcggt ccaggatgcg ttgctcctct
    19741 ttcgacagcg gtgtttcttt tgaggctccc gtgatcacac tgagattttc aagttccagg
    19801 tcggccattc gcttcacgaa catcacgctg gccagtacca cgccaatgcc cacagccgtg
//
LOCUS       DATIAL010000185         3566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1431726, whole genome shotgun sequence.
ACCESSION   DATIAL010000185 DATIAL010000000
VERSION     DATIAL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431726"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(611..1396)
                     /locus_tag="VLA60_05860"
     CDS             complement(611..1396)
                     /locus_tag="VLA60_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08919.1"
                     /translation="MRHSQTSTRNPAIKDWTSKYGKRTNLQFFNDAFAALQKQDIGNR
                     GLMTVGLIGSRDLPGHTLRTAQSMLRWDLRPSFWSHVFMVAEPVTSRTSLRSLPILEV
                     PLHPRNGMFPRPECNGINEGTLGLYEDKDIDANVGLVAVGMTDEEAKKLKKRARDWNQ
                     DRVRYNFWEMLGVWQSYLWSQGAKRNPLREGMPIAASSYIEFIFEGVGLGVTPGTSER
                     NSAPEHLWNAACWWHKAFKEQDRKVAGMFVARDRGCAMASCEE"
     gene            complement(1480..2928)
                     /locus_tag="VLA60_05865"
     CDS             complement(1480..2928)
                     /locus_tag="VLA60_05865"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSF08920.1"
                     /translation="MNMWIVPGAPLMEGRGWRIWCSQKGEESFSQPQAEVRHKNAMVP
                     FQSTWTLLPQLPGLKRRMGVMALTLNTPGPPEGALYSVTLATNQERQTFHWWTMPYHL
                     TAQPVTFLFASCFWHNNDREGFYRSGLQELGQLAHPHFKLLLGDQVYGDWPNAWDLGD
                     EGIELYAKRYAQYWGDGAYREALQTCPNFFSCDDHEYWNDYPEKQIQLPQTWDSKNRQ
                     KYGGGAEELYYQYQRCLNPDEARWYRFEIDPVSFFITDTRSEREGCNDKGISHFMSEE
                     QWVALEAWQQGLTGPGVLTLGQPLFQKDGDFRDHSLSNFKQDYARLWRVIERSLAGRN
                     AQQQPHDILILSGDIHTGRYAEAHGPFSDAPYGVPEFIASPAAMINPGNTKPEAPPGK
                     IRILPNVEGKESVWRIDPQTNIDIMTIQNNVGLVRMVPGSQTGGSPRVRFELELWSLP
                     AKVIEIDWDETAPPQGLGGPLTRLWNKELLLR"
     gene            complement(2956..>3566)
                     /locus_tag="VLA60_05870"
     CDS             complement(2956..>3566)
                     /locus_tag="VLA60_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF08921.1"
                     /translation="VAYYHQHPSAYLLHLGRKHQTPLFREYLTLSLLTHITRRFLSGK
                     PPLRAEQLARAQGVPLTLVESLIDDLVKGQMVVKGDKPKGLVLAQAPENIPVVEVLRV
                     VQHQPHDGPQAFTVGTDSISRVLIRRDAAVKAALEGMTLRDLVRRQPEVLEEENGKDH
                     SQGQDPGGAFHPESEGEVPGPSGFLQETTQADPPVLGKGPAR"
BASE COUNT          843 a         1003 c          879 g          841 t
ORIGIN      
        1 atcgcttatg atggacagtg acacgaaaaa agtaaagcct gtgcagaagg aatggaacag
       61 gaaaatgatg cttcttccaa acattctcca aaacattctt gcctttgagt catgccagta
      121 gagactgcct gcaacaagga attacctgca gtctctagcc cttcccctgt ccgacgcctg
      181 ccgcccgtcc ccctggtcgg acgctctttg tcttgccggg ccttgtctga gcgcagcgag
      241 ttggtccgcc ctccaatgcc tttcgtccga tcaatcagat gagacgggct gggcgtcagt
      301 ggttttgggt ccttttcccg aaagaaaagg acctcggctg ccgggccgaa acccggcacc
      361 tctcgtccac tcacataaat gccagtgagc agaaaaattt atagctcggg gaggaaggaa
      421 aaaacttggg actcggcggc acaaagagga agaggttttc gcttcgagat acgataagac
      481 acggtgtcat tcccgacatt attcatcggg aagaagcctc ttaattatca gatggattcc
      541 cgataacagg tgcggggatg acggaattcc tgccacggcc ttaacggtaa catcctcata
      601 catatagaaa ctattcctcg cagctggcca tggcacaccc ccggtcacgc gcgacaaaca
      661 tcccggccac tttccggtct tgttccttga acgccttgtg ccaccagcag gcggcattcc
      721 agagatgctc cggggcacta ttgcgctcgg aggttcccgg cgtcacaccc aaacccacgc
      781 cttcaaaaat aaattcaata tacgacgagg cggcgatggg cataccctcc cgcaacggat
      841 tgcgcttggc cccttgcgac cagagatagc tttgccagac tcctaacatc tcccaaaagt
      901 tgtaacggac acgatcctga ttccagtccc tcgcgcgttt tttaagcttt ttggcctcct
      961 catccgtcat accgacagcc acaagtccga cattcgcatc gatgtctttg tcctcataca
     1021 ggcctaatgt gccttcatta ataccgttac actccggcct tgggaacatc ccgttgcgtg
     1081 gatgcagcgg aacttcaaga atcggcagag accgcaacga cgtgcgagag gtcacgggtt
     1141 cggccaccat aaacacatgc gaccagaaac tcgggcggag atcccaacgc aacatggact
     1201 gtgccgttcg caacgtatgt ccgggaaggt cacgactgcc gatcaatccc acggtcatca
     1261 atccacgatt gccgatgtcc tgtttttgca aggcggcaaa cgcatcatta aaaaattgca
     1321 ggttggtgcg tttgccatat ttgctggtcc agtctttgat agccggattt ctggttgagg
     1381 tttgagaatg gcgcatgatg aattcctttc tagatatggc tcagaaaaca ctctatagac
     1441 atgcctgggc acccatggct gaaatgtcaa tttctccgat taccggagca ataactcctt
     1501 gttccacagg cgcgttaagg gaccacccag accttgaggg ggagccgttt catcccaatc
     1561 aatttcaata actttggccg gcaaactcca taattccaac tcaaatcgta ctcgtggcga
     1621 tcctccggtc tgacttccgg gaaccatgcg cacgagaccc acattattct gaatagtcat
     1681 gatgtcgatg ttcgtctgag gatcaatccg ccacaccgac tcctttccct ccacattggg
     1741 aagaatccgg atcttgcctg gcggggcttc gggtttggta ttgcctggat taatcatggc
     1801 tgccggagaa gcgatgaact ccggcacccc ataaggtgca tcggaaaatg gcccatgggc
     1861 ttcagcataa cgtcccgtat gaatatctcc cgacaggatc agtatgtcat gtggctgctg
     1921 ttgagcattc cgccccgcta aggaccgttc gatcacccgc cagagacggg cataatcctg
     1981 tttgaaattc gataaggaat ggtcacggaa atccccgtct ttttgaaaga gtggttggcc
     2041 caacgtcaaa acgcccggtc ctgttaatcc ttgttgccag gcttccagcg ccacccattg
     2101 ctcctctgac atgaaatgac tgatgccttt gtcgttgcac ccttcgcgct cggaacgggt
     2161 gtcggtaata aaaaatgaga ccggatcgat ctcaaaccgg taccagcgag cctcatcggg
     2221 attcagacat cgttgatact ggtaataaag ttcctccgcc cctcctccat atttttggcg
     2281 attcttcgaa tcccaggtct gaggcagttg aatttgtttt tcaggataat cgttccagta
     2341 ttcgtggtca tcgcaggaaa aaaaattcgg acaggtttgc aaggcttccc gataggcgcc
     2401 atccccccaa tattgggcat accgcttggc atagagttcg atgccctcat cccctaaatc
     2461 ccaggcattc ggccaatctc catagacctg atcacccagc aataatttaa agtgcggatg
     2521 agccaactgc cccaactcct gaagaccgga ccggtaaaat ccctcacgat cattgttatg
     2581 ccaaaagcag gaggcaaaca gaaaggtcac cggttgggcg gtgaggtgat acggcatcgt
     2641 ccaccaatga aaggtctggc gctcctgatt cgttgccagc gtgacagaat agagagcgcc
     2701 ttccggcgga cctggagtgt tgagcgtcag ggccatcacg cccattcgtc tcttcaatcc
     2761 tggaagttgg ggaaggagtg tccatgtact ctggaacggg accatcgcat tcttatgccg
     2821 cacctcagct tgtggctgag aaaaactttc ctcgcctttt tgcgaacacc aaatccgcca
     2881 gcctcgccct tccatcaacg gcgcacccgg gacaatccac atattcatct ttttcccctt
     2941 atcagcatag gctgattacc ttgcaggccc ttttccgagg acaggaggat cggcttgtgt
     3001 ggtttcttgg agaaagccgg aaggaccggg aacctcaccc tccgactcgg ggtgaaaagc
     3061 cccaccggga tcttggccct gggaatggtc ctttccgttc tcttcttcaa ggacttcagg
     3121 ttgacgtcgt actaaatccc gcaacgtcat gccttccaat gcggctttca ccgccgcatc
     3181 ccggcgtata aggacccgac taatggaatc tgtacccact gtaaatgcct ggggcccgtc
     3241 atggggttga tgctgcacca cccgtaacac ctcgaccacc ggaatattct ctggtgcctg
     3301 cgccaatacc aatcctttgg gtttatcgcc tttcacgacc atttgccctt tcaccagatc
     3361 atcgatgagg gattccacca acgtaagagg cacgccttgt gcgcgggcta actgctctgc
     3421 cctgagaggc ggcttccccg ataaaaaccg gcgggtaata tgggtgagca atgacagggt
     3481 gaggtattcg cgaaagagtg gagtctggtg ttttcgacct aaatgtaaga ggtatgcgga
     3541 tgggtgctga tgataatagg cgactt
//
LOCUS       DATIAL010000186        15990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_142031, whole genome shotgun sequence.
ACCESSION   DATIAL010000186 DATIAL010000000
VERSION     DATIAL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15990
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142031"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VLA60_05875"
     CDS             complement(<1..461)
                     /locus_tag="VLA60_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HSF08922.1"
                     /translation="MKTFQSGERIHLIDKKERPYAVTLKAGEIFQHSGDRVSHDAIIG
                     KPDGTVVQFSNGKLMVAVHPTLAEYTLKMPRGAQVIYPKDLAVIPMWADIYPGARVFE
                     AGVGSGALTMALLRAVGDRGCVVSYEMREDFAATATKNIERFMGKVPNHFL"
     gene            819..1508
                     /locus_tag="VLA60_05880"
     CDS             819..1508
                     /locus_tag="VLA60_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSF08923.1"
                     /translation="MKRLFTVIGLGRFGYSVAQGLVTKGCEVLAIDKDEEKIQAISDI
                     ATFAVQCDATDERALKAVSAQNVDVAVVSIGENIEASILIVQTLKEMGVKSIVAKAVA
                     PIQGKILMNLGVDEVIYPERDAAIRLAHRLVSPSVLEYLELAPGFSVEEVSVSESLSG
                     LSLEDVKLRGKHNLNVIGIKKQVTRMVKGRMMKEEIFNFTPKPDDVIEKGDVLVMIGR
                     EEDLDRFCNNV"
     gene            1918..3063
                     /locus_tag="VLA60_05885"
     CDS             1918..3063
                     /locus_tag="VLA60_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF08924.1"
                     /translation="MEYQTVLDEPTETELATIKTEGTKPSIRGFGPALESLYFRSFGS
                     RPLLNKDSEIELAKEIDQASRAIRVIIKQSLHLTHPLKKGPEREETIALLKETFTLSG
                     FSTPAIEKLKSVLLALAQTHPSVSSSIQDLCQQLQKAKFKLEKAKSELVQRNLRLVVD
                     IAKRYTGHGLAFLDLVQEGNIGLMKAAERFQYRKGFKFSTYATWWIRQGITRSLADQS
                     RTIRVPVHLNEISNKIARATKRLTQQHARPIQLDDIGEALKLSVEKVHDTIQAFQDPL
                     SLETPVGDGETFLTDFIPDMGTLTPDGPVSRQETNQQVERILDSLTPREQMVIRLRFG
                     IGQDEPWTLEEVGQSMSVTRERVRQIEAKALQKLKEPAMKDMLYSIK"
     gene            3773..4720
                     /gene="lpxC"
                     /locus_tag="VLA60_05890"
     CDS             3773..4720
                     /gene="lpxC"
                     /locus_tag="VLA60_05890"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HSF08925.1"
                     /translation="MRHQQTLETSSFLSGIGLHSGNPASIAIHPAPVNTGVVFVKHIA
                     DQIQSCPASIAFLGQTDHCTTLQVDDFDIQTVEHVLSALAGLEIDNAYVELLGSEIPA
                     ADGSATPFVDMVYQSGVLTQEEPRKYLKIIQPITVEDGHRSISVLPSSLPQITYFIDF
                     PHPLIQQQEYHHFCTPQDYGKNIAGARTFAFRKEVEFLWSRGLGLGGSLHNTVVFAET
                     GLVNDDALRFSDECVRHKVLDLIGDLSLLGIPVIGHFFAKCAGHALHTQLVQAIMHNP
                     DKWIMLNAGESGKDGIFNGQFSSNPTIHLPELQPAFHAF"
     gene            5052..5396
                     /locus_tag="VLA60_05895"
     CDS             5052..5396
                     /locus_tag="VLA60_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08926.1"
                     /translation="MHIYTIKRGAPQLGSIELESVKLYTLDGKYARVLFHYQGEAIHK
                     AILQYLRNEFGKTNDPYGSMIRGLSQQYTWRGPETEITLTYHGFRERGTLTVEGRIYA
                     PLFLDTLSENSY"
     gene            5989..6762
                     /locus_tag="VLA60_05900"
     CDS             5989..6762
                     /locus_tag="VLA60_05900"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HSF08927.1"
                     /translation="MSNHIRSDLLALNPHQTVSIQRVGMGHHQIVIVDNFYQFPDEIL
                     KVALSLPYTDRFEIVGNFPGVRARLNYEHLELVESLSELWGCPLFTFFSPQPVVFQGI
                     KTHNYALNVGQRQPHIDQDITAMVYLNPADSCTGGTGLYRHRPTGLERVPPIPDGTIR
                     QLANQLELSDEFFTSSEGYENFQNSMIFNPLFACRDNRYINDGNEYWELLKLIEMRPN
                     RLMLFDGRCFHSQYIQSGHYNQAFRVNQILYLSQKEKVL"
     gene            complement(6972..8318)
                     /locus_tag="VLA60_05905"
     CDS             complement(6972..8318)
                     /locus_tag="VLA60_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HSF08928.1"
                     /translation="MKKHEFRTEPLSTASMPSGVLHLVVNEGAERFSYYGMRSILVVF
                     MTGMLLNAEGSLDPMGEAEAKSYFHLFASGVYFFPFLGAILADAFWGKYKTIILLSLV
                     YCGGHVVLALDHTRFGLLIGLALIAIGSGGIKPCVSANLGDQFGLANGHLLTKAFAWF
                     YFAINVGAFGSVMLTPWLLEHFGPGLAFGVPGILMFVATIIFWSGRTHYAHIPPDRLG
                     VFQALSRPQTWRAIGKLAGIYVFVAFFWSLYDQTSSAWVLQAQHMDRVWMGVEWLPSQ
                     IQAVNPVLIMVFIPLFTYVVYPVLNRMVLLTSLRKMAMGFFMAVGAFLISAYIEQQIE
                     SGMEPAIGWQLLAFVVITAAEVMVSITCLEFSYTQAPLKLKSLLMGLFLLSVSLGNGF
                     TAFVNFWIQNSDGTVALTGPDYYWFFAGVMFLVACLFLLVMKGYEEESYLQGTEPG"
     gene            8441..10399
                     /locus_tag="VLA60_05910"
     CDS             8441..10399
                     /locus_tag="VLA60_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSF08929.1"
                     /translation="MPTPFPPFPGIILLASLSTLRVLLPTLVQAEESSVTTLDTMIVT
                     GSAQPTQPHQSTQSITTLNAEQIAPLQPNRVTTILQQVPGLHTDEMSGRSGISSVYLR
                     GADPNFTLIMLDGVPMNDSTNQRGGSVDLSTIPIDRIDRVEIVRGPLSAFYGSEAMAG
                     AINFITKSASTGPSFRLLGEGGRFDYVKGLVQTGGSLGPLSANLSLSHTQNGEQIEKD
                     SFLMDSAGWNFSLQTDPNFDIQLNGQYTDSTVHSFPEGSGGSELALLRETERRKTQEF
                     LTGLSSSFHTPSGWQHQIFLSITRRLQEVSNPGILATPTTFALPPADFETLYTRFQAR
                     MTTTWTINPQWKFSLGEQLTHERGKRNGTQDLSSFGGSSNQPDDFAIQRTLGGTFAEL
                     TSVWWQKLTLNSGVRADISQGFQPKITPRIGAKYQLLASIQIRGGYGRGFKLPSLSSL
                     GDPLIGNPSLQPETSVGWDLGLDYHTVDERFTATIEYFHNRFRNLIDLDPDLLNQGII
                     RLANIDEAETHGWNIALSLTPLPAVSFQSSLTHLTTQVTETGAQLRNRPKWRGWLGVT
                     MKLSSTLNVKSQVTWVSSSFDFQVPTQTTRVGGFAKADITVNYHPFTAWSCYAALENV
                     TNSSYEQFAGFPAPPLTFRLGLEYRPEF"
     gene            10587..11573
                     /locus_tag="VLA60_05915"
     CDS             10587..11573
                     /locus_tag="VLA60_05915"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF08930.1"
                     /translation="MQLILPLDPVQDEERSTIFARIKSCKSLPILSPLASTILKMCQN
                     EETNASELATIISQDPGMAAQILFMANSGYYGGSRHKVTSIGQAVTLLGFSTIANLAL
                     SFCFYRLIKDMKEVPTSGIDHVKFWRRSILASVAARAIGTHFAFPDVELIFLGALLQD
                     IGLLAFNEVASTEIASLQDHNQDNHANLHLYELEHLGVDHAQVGAWLAQHWQLPEEFQ
                     VAILYSHTPELFETPLDFQALVDTVALSGIIADIWINPDTEAAIASARKNSQGRLTLQ
                     PTDWEPILGHMMTGIPQIASFFHSRIGNFEDIAHIHQVALQQLSSTDPHLFS"
     gene            complement(11600..12757)
                     /locus_tag="VLA60_05920"
     CDS             complement(11600..12757)
                     /locus_tag="VLA60_05920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSF08931.1"
                     /translation="MLGSWSIGKKDHHHENGAQKEPVANGSRAAEISGESDKAWESLA
                     RVLRCLGRHAFELDQLSEEDIEKEFERWARHVLVGAQIDADDSKKREASPRRDWGALT
                     QFVNGHRQKEKSYVTRNLQDMRTVLCEFTNIMGRAFVEDQEIDQKVTNHLVQLRTATE
                     DGSFHDVKQALLAVVEGISALVDERKERQQQRLENLGEKLRLVEEELGGARKQMTLDA
                     LTQLYNRGALDLQLERTASMSFFSGTSVCILMVDVDHFKHVNDTYGHPAGDVVLQHVA
                     KRLVSTFPRKTDFIARYGGEEFCVLLQGADGELSQRLGERLLDAIRGEGFPYQDITIP
                     VTASIGLAELLPGETVLSWLERADRALYRAKDNGRNQLCLATEHAGVGKKG"
     gene            13237..13749
                     /locus_tag="VLA60_05925"
     CDS             13237..13749
                     /locus_tag="VLA60_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HSF08932.1"
                     /translation="MFGTKEPLLVCGVPEMSRRDLSRAPNGLEVLCPIGDPGNLGALL
                     RCCRAFDVQTVVLLQEAVHPFHPKVIRASSGAVFVQSLEWAGSITDLNAPETLQWITA
                     LDLHGTNMSTVQWSKHVRLLIGEEGVGIPPFQFLERLCIPQIHPAIPLNATVAGSIAL
                     YAYRQQHSQV"
     gene            13830..14114
                     /locus_tag="VLA60_05930"
     CDS             13830..14114
                     /locus_tag="VLA60_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HSF08933.1"
                     /translation="MHTPQPPQDQGLTMIQPGRYRHYKGKEYQVIGVAKHSETEEDLV
                     VYRALYGESGLWVRPAKMFREKIEIEGKLVFRFEWIGGANGEPDTTTINP"
     gene            complement(14291..14779)
                     /gene="purE"
                     /locus_tag="VLA60_05935"
     CDS             complement(14291..14779)
                     /gene="purE"
                     /locus_tag="VLA60_05935"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSF08934.1"
                     /translation="MAESSFRVAILMGSKSDWDTMRLAGETLTKLAITHESQVLSAHR
                     ASDALGEYVRAAEAGGVQVFIAGAGGAANLAGVIAATTQLPVLGVPLQSMALQGLDSL
                     LSIVQMPRGIPVGTLAIGSAGAVNAALLAAAILALTDASLSKRLNAYREEQTRAVLAE
                     QL"
     gene            15030..15665
                     /locus_tag="VLA60_05940"
     CDS             15030..15665
                     /locus_tag="VLA60_05940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HSF08935.1"
                     /translation="MAIRLGDEAPNFTAETTQGTVNFHEWLGDGWGILFSHPKDFTPV
                     CTTELGTMANMTEEFKKRNVKVLAISVDPLDSHKGWIKDINETQHCTVSYPIIADPEK
                     KVATLYDMIHPNALDNMTVRSVFVIGPDKKVKLTLTYPASTGRNFNEILRVIDSLQLT
                     AKHKVATPANWKDGEDCIITPAVTDAEIPALFPKGHRAVKPYLRFTPQPNR"
BASE COUNT         4424 a         4205 c         3346 g         4015 t
ORIGIN      
        1 tgaaggaaat ggttaggtac ctttcccatg aaccgttcaa tgtttttcgt ggccgtggcc
       61 gcaaaatcct ctcgcatttc atagctcacc acacagccac gatcccccac cgcacgcaaa
      121 agagccatcg ttaaggcccc agagcccacc ccggcctcaa agaccctggc tcctggataa
      181 atatcggccc acattggaat gacggctaaa tctttaggat aaatcacctg agctcctcga
      241 ggcattttca aggtatactc agctaacgtt gggtgaacgg ctaccattaa ctttccattg
      301 gaaaactgga ccactgtccc atccggtttt ccaataatgg catcatggct aacccggtct
      361 ccactatgct gaaagatttc tccggctttg agagtaacgg catacggccg ttctttcttg
      421 tcgattaaat gaattcgttc tcccgattga aatgttttca taaggcacgc attctagcaa
      481 ggcttttcct cgctttgata cccaccataa taatggagcc ttcttaggtg aaccaactcc
      541 acaactaatc gaccattaaa tgggatcatt cttgacaccc tccccattca gacgtatgat
      601 taatcaactt cagtctttac atacccatcg ccaccaaaat ctcccatttt tcagatcatt
      661 caaatttgca agtggcactt gtgtgaatgt tctcaacata caaaaaacca aaccccggct
      721 ggaacgaatc aggaaatttc cttaaccaca gcactctcgg ctcctcttga tgcaggaata
      781 gtcgcatgat gaaaacaatg gtcgttggtt aatttaccat gaaacgcctt tttacagtta
      841 tcggacttgg gcgcttcggc tatagcgtgg cacaaggatt ggtcaccaaa ggatgcgaag
      901 tacttgccat cgataaggat gaggaaaaaa tccaggcgat cagtgacatt gcgacctttg
      961 ccgtccaatg cgatgcgact gatgaacgcg cgttaaaagc agtcagtgct caaaacgtcg
     1021 atgtcgcagt cgtcagtatt ggggaaaaca ttgaagccag cattcttatt gttcaaacac
     1081 tcaaggaaat gggggtaaaa tccattgtgg cgaaagccgt ggccccaatc caggggaaaa
     1141 tattaatgaa cctaggggtc gatgaggtca tctaccctga acgagatgcg gccatccgtc
     1201 tcgctcacag gctggtctca ccgagcgtac ttgaatactt agaattggcc cccggtttta
     1261 gtgtggaaga agtgtccgtt tcagagagct tgtccggatt gagtcttgag gatgttaagt
     1321 tacgcggaaa gcataacttg aatgtgattg ggataaaaaa acaagttacc agaatggtca
     1381 aagggcgcat gatgaaagag gaaatattta acttcacccc aaaacccgat gacgtcattg
     1441 aaaaaggtga tgtcctggtc atgattggcc gagaagaaga tttagaccgt ttttgcaata
     1501 acgtgtaaaa aatataacag ttccgtcaat tacctctgca ctttttattt cctcacccgc
     1561 tctctcctat aaattttggg tttttcccct ttcgcactat ccagggaatg cgatgggcta
     1621 ccgatttctt caatagataa cctattagtc caaaaggtag gataaatttc acagatagta
     1681 tttttatcct tatgtttcat actgttatcc atttgatgaa atgagagatt cataatcaaa
     1741 ccatcatagc gaaaattcag cgttccccaa aaaaggcatt aagttgcttc ccattgctgg
     1801 ctaaacgggc tgaaacatac tcattggctt ggtttttgct tttgtaatct tactgaagat
     1861 ttacattgat gttgaactat aggctatcgg aaacctgaac tcccagggga aactattatg
     1921 gaataccaaa ctgttttgga tgagccaaca gaaaccgaac tcgctaccat aaaaactgaa
     1981 gggaccaaac cttcaattag aggattcggg ccagctctcg aatcattata tttccgatct
     2041 tttggcagca gacctcttct caataaagat tccgagatcg aattagcgaa ggagatagac
     2101 caagcgtcca gagcaatcag ggtcattatt aaacagagtc ttcatctcac tcatccgttg
     2161 aagaagggac cagagagaga agaaacaatt gctcttctca aagaaacgtt taccttaagt
     2221 ggtttttcga ctcccgccat tgaaaaattg aaatccgttc tgttggcctt agcccaaaca
     2281 catccctccg tgtcctcatc cattcaggat ctctgccaac aacttcaaaa agccaaattt
     2341 aaactagaaa aagcgaaatc cgaactcgtt cagcgaaatc ttcgcctggt tgtggacatc
     2401 gcgaaacgat atacgggtca tggattggcc tttttggact tagtccaaga gggtaatatt
     2461 gggctcatga aggcggcaga acgatttcaa tatcgcaaag ggtttaagtt cagcacttat
     2521 gccacatggt ggattcggca agggattacg cgatcactcg ccgaccaatc tagaaccatt
     2581 cgtgtccctg tccatttaaa tgaaatctcc aataagattg ctcgtgccac caaacggttg
     2641 acacaacagc atgcccgccc aattcagtta gatgacattg gagaggcgct caaactgagt
     2701 gtggaaaaag ttcatgacac cattcaagcc tttcaggatc ctttatcgtt agaaaccccg
     2761 gttggagatg gagaaacctt tctcacagac ttcattccag atatgggaac tttgacgccg
     2821 gatggacctg tctcacgcca agaaacgaac caacaagtcg aacggatttt ggattcgttg
     2881 actccaaggg aacaaatggt gatccgcctg cgttttggca ttggccaaga cgagccatgg
     2941 acccttgaag aagtcggaca aagtatgtcg gttacccggg aacgtgtccg ccaaattgaa
     3001 gccaaagccc ttcaaaaatt aaaggagcct gctatgaagg atatgctata ttccattaag
     3061 tagaaaatgc tgcgttcatt ccctcatccc cctttgcatt caaccatttc ttttaggggt
     3121 atttgccttt tctgaccgag gacttgtcaa ccagccaatt accgccttaa catagggcta
     3181 ttcggtgctt gtttcattca tgtataaaga ccaccgcact tgttgccatc agaccctatt
     3241 tacagacttc gacaagaatt cagaggccac cttgaagaat tctacaccca tctgaatctg
     3301 gccccgccat accatagtat tgaaaaagcg gttcaacatt tttccaatac gctcagaaca
     3361 agacctgaag aattctctcg gggactgcta caagatgcgt caaaaaaatg gaatgtattt
     3421 caggaaattt tcttagcctc tggcttgcaa aagaagcatc gaggaatcat tcaacaatta
     3481 gctcattctc atcatacgac tttagttgat gcacctgaat cgcgtcgctt ccttcaaaat
     3541 tttttaacaa actcacaaga atccacttct aagccattaa atcatccacc atcgcagcca
     3601 atccttcccc actaaccaat ccttagtaac catttagtga aaaaagtata catttgtttg
     3661 gtttctgcaa cactcaaaaa cagaaagtcc taatttcaaa tacaaacacc gccaaatcat
     3721 cgggtagtac aaattttgca tagtttttaa tattaaagat tcacaggaac ttatgcgtca
     3781 tcaacaaaca ctcgaaacat catcattctt atctggcatt ggtctccatt cgggtaaccc
     3841 tgcctcaatt gccattcatc cagctccggt taacaccgga gtggtctttg tgaagcatat
     3901 agctgatcaa atccagtcat gcccagccag cattgccttt ttaggacaaa ccgatcattg
     3961 caccacactt caagtggatg attttgacat acagacggtt gaacatgtat taagtgcact
     4021 cgctggccta gaaatcgata atgcctacgt ggaattgctt ggcagtgaaa tccctgcagc
     4081 agacggcagt gccactccat tcgtcgatat ggtttaccaa agcggggttc tcacccagga
     4141 agaaccgcgc aaatacctta aaattattca gccaattacc gtggaagatg ggcaccgatc
     4201 aatcagtgtt cttccctcgt ctctcccaca aataacctat ttcattgatt ttcctcatcc
     4261 cctaatacaa caacaagaat atcaccattt ttgcacgcca caggattatg gcaaaaatat
     4321 tgccggagcc cgtacattcg cctttcgaaa agaggtggaa tttttatggt cgcgaggact
     4381 ggggcttggt ggttcactgc acaatacggt cgtctttgct gaaacgggat tggtcaatga
     4441 tgatgcctta cgattctcgg acgaatgtgt tcgacataaa gtgctcgacc ttattggcga
     4501 tttgtcatta ttaggaatcc ccgttattgg tcatttcttc gcaaaatgcg ccggacacgc
     4561 actccatact caattggtcc aagccatcat gcataatcct gacaagtgga tcatgttaaa
     4621 cgcgggagaa tctggaaagg atggcatctt caacggacag ttctcctcca atccaacgat
     4681 tcacctccct gaactccaac ctgcctttca tgcattctaa cgcctctctt tctttcctca
     4741 ctatgtgccc taaactaaaa ttgcgtttgc cttctgccta tcaataggaa ggaagaaatt
     4801 cttttctcca ttgtcttcat cccctacgcg tggtagcgtc tggcataatc atgagactcc
     4861 gatcatttgt ccgccctaat agaacaagca ggcttaaatt acttttcatc atttgcgtct
     4921 gccttttctc gtggacgcct gtcttaagcg ctacaccaat agaaaaagat cccaaaggat
     4981 tcttcggcag cctctggggc cattcgctcc aagatcgcac agatctaaaa gaaatcgatt
     5041 caatagattc attgcatatc tatacgatta aacgcggagc accacagctt ggatccattg
     5101 agttggaatc cgtcaaactt tataccttag atggaaagta tgctcgggtc ctctttcatt
     5161 accagggaga agctatccac aaagccatct tacagtacct gagaaatgag tttggaaaaa
     5221 caaacgaccc gtacggatcc atgattcggg gattaagtca acagtacacc tggcgcggac
     5281 cagaaaccga aatcacgctt acctatcacg gatttcgaga gcggggaact ttaactgtcg
     5341 agggtcggat ctatgcccca ctgtttcttg acacactttc tgagaattcc tattaatcgc
     5401 tgaatttttt gcttggtcac caacaatgaa ttatcagaaa tcgtgataac ccttcgcaga
     5461 cctcgcatgg agccaatcct gtgcttcttt tcggtttacc tttactcgca atgatgacga
     5521 gggccaaaga ataaatgctt tataaaatca gtgagtaata tttgcaaata tgattttatg
     5581 ctacagcgtg gttccagttt cgccaaaact aaacatttcg gcagcttaat caggagcata
     5641 gcctaataat taagccatcc tgccgaatac ttccagctcg actaagccga cccgatgact
     5701 atttcatccc acaccaaatt cgggttagag agtgaggcga gtaaggcact attaagaaaa
     5761 gagatcgtaa ccgggagaac aacattttcc tatgtcaggg gatgtcccca tacagtgagc
     5821 cctgaacaat actgaactaa ttcttagatg acagcctcct cactgccgca tggtaatcct
     5881 tcctctctcc gacaaaattc tatgtaaagc ccttgactcc tcctgaaaca aggttcacta
     5941 taggcccgtt tttcaacgtc atcctagccg aataccaata gggactccat gtccaatcac
     6001 attcgctcgg atcttctcgc cctcaatccc caccaaacgg tatcaattca gcgcgttgga
     6061 atgggccatc atcagattgt aattgtagat aatttctatc aattccccga tgaaatcctg
     6121 aaagtcgcgt tgagcctccc ctataccgac cgcttcgaaa tcgtgggaaa ttttccagga
     6181 gttcgagccc gccttaatta tgagcacctg gaacttgtgg aatctctaag cgaactttgg
     6241 ggatgccctc tcttcacatt tttttctccc caaccagtcg tctttcaagg tatcaaaacc
     6301 cacaactacg cgctgaacgt tgggcaacgg caacctcaca tcgatcagga tatcaccgcc
     6361 atggtctatt tgaatcctgc agattcatgt acgggtggaa ccggcttata tcgccatcga
     6421 ccgacaggac tcgaacgcgt accacctata cccgacggga caatacgaca acttgcgaac
     6481 caactggaac tcagcgacga atttttcacc agctcggaag gatatgaaaa cttccaaaat
     6541 agcatgattt tcaatccact cttcgcttgt cgagacaata ggtatatcaa cgacggaaat
     6601 gaatattggg aattactgaa acttatcgag atgcgtccaa accgattgat gttgtttgat
     6661 ggacggtgtt ttcattcgca gtatatccaa tccggccact acaaccaggc ctttcgggtg
     6721 aatcaaattc tgtacctcag tcaaaaggaa aaagtgctct gattctttaa tacctccgcc
     6781 tccccctccc tacaaggctt ctttcgtttt tatgagactt ccgactcctt ctagtcaaag
     6841 cttgaacaaa tgttagaaca tcataagggt tcttggtcta catctggttt atagaatagg
     6901 agagaatatt tatctcaacc aacggtaaaa aatgcgattg tccgaacgtg tatagatccg
     6961 ctctggtcag atcatcccgg ttccgttcct tgcaagtacg attcttcctc gtaccctttc
     7021 atcaccagaa gaaacaggca ggcaacaaga aacatgacgc ctgcaaaaaa ccaatagtag
     7081 tcggggccgg tcaacgcaac agtcccatct gaattctgaa tccaaaaatt tacaaaggcg
     7141 gtaaatccgt tccctaagga cacggaaagc aaaaataagc ccattaggag cgatttgagt
     7201 ttaagtggag cctgggtata ggaaaattcg agacaggtaa tagacaccat gacctccgcc
     7261 gccgtaatca ccacgaaggc caataactgc cagccaatcg ccggttccat ccccgactca
     7321 atttgttgct caatataggc tgagatcaga aacgccccaa ccgccataaa aaagcccata
     7381 gccattttcc gcaaagatgt gaggagaacc attctattta aaaccggata caccacatag
     7441 gtaaacaaag gaataaacac catgatcaag accggattca ctgcttgaat ttgcgaaggc
     7501 aaccattcaa cacccatcca gacccgatcc atatgttgag cttgaagaac ccacgctgaa
     7561 gaggtttggt catagaggga ccaaaaaaat gcgacaaaca cataaatacc cgccaacttc
     7621 ccaatcgctc gccatgtctg tggacgagaa agcgcttgga atacacctaa gcggtcagga
     7681 ggaatatgcg catagtgggt ccggccagac caaaaaatga tggtcgccac aaacatcaag
     7741 atgccaggca ccccaaatgc caggccgggg ccaaaatgct ccaacagcca gggagtgagc
     7801 atcacggatc caaatgcccc aacgttgatg gcaaagtaaa accaggcaaa ggccttggtt
     7861 aaaagatgtc cattggccaa accaaactga tcccccaaat ttgctgacac acagggttta
     7921 attcccccgg agccgatcgc aattaaagcc aatccaataa gtaggccgaa ccgggtgtga
     7981 tccaaagcca acacgacatg gccgccacaa tacaccagag aaagaagaat aatggtttta
     8041 tatttccccc aaaaagcatc ggccaatatt gcccccaaaa atgggaaaaa gtacactccc
     8101 gaggcaaaaa gatgaaaata acttttcgcc tctgcctcgc ccatgggatc caaacttccc
     8161 tctgcattca gcaacatccc cgtcataaac accaccagaa tggaacgcat cccgtagtaa
     8221 ctaaatcgct cagcgccttc attgacaacc aaatgcagga ctcccgatgg catggaagcc
     8281 gtcgacaatg gctcggttcg aaattcatgt tttttcatga acaaactttc atctgtaaga
     8341 agtggacaag gattgaaaat tcctatagtt cctatccggt gagaacggct gggtcagtcc
     8401 gacaagcaaa cctccgttcc cacaggaatg tcattgtata atgccgaccc ctttcccgcc
     8461 attcccaggc ataatcctct tggcgagtct ttcaactcta cgggtgttgc ttcccacgct
     8521 ggtacaggcc gaggaatcct cggttacgac gcttgatacg atgattgtca caggttccgc
     8581 ccaacccacc caaccccacc aatcaactca aagcattacg actctcaacg ctgaacagat
     8641 tgcccctctt cagcccaatc gggttaccac catcctccaa caagtcccag gactccatac
     8701 cgatgagatg agcgggagga gtggaatcag ctcagtgtat ctccggggtg ccgatcctaa
     8761 cttcaccttg attatgctgg atggggtccc catgaatgac tcaacgaacc agcgaggcgg
     8821 atcagttgat ctttccacta tccctattga tcggatcgat cgagtagaaa ttgttcgggg
     8881 tcctctctcg gcattttatg gatctgaagc catggcggga gcaatcaatt ttattaccaa
     8941 atctgcttcc acgggtccct cttttcgcct cttgggcgaa ggcggtcgat tcgattatgt
     9001 caaagggctt gtccaaacgg gaggatctct gggaccattg tccgccaatc tttcgctgtc
     9061 acatacccaa aatggagagc aaatcgagaa agattcattt ttgatggact ccgctggatg
     9121 gaatttttcc ctacagacag atcccaactt tgacatccaa ctcaacgggc aatatacaga
     9181 ttcaacggtc catagttttc cggaaggcag tggtggctcg gaattggctt tgctccggga
     9241 aaccgaacga cgaaagaccc aagaattcct gacaggtttg tcctcctcat ttcacactcc
     9301 ttcgggttgg cagcatcaga tatttctgag catcactcgg agacttcaag aggtgtcaaa
     9361 ccccggaatt cttgcgaccc caactacctt tgcgctgcct ccggctgact ttgaaaccct
     9421 ctatacccgg tttcaagcac gtatgaccac aacatggacg atcaaccccc aatggaagtt
     9481 ttcacttggt gaacagctga cgcatgaacg aggaaaacgg aacgggacgc aagacttgag
     9541 ttccttcgga ggctcctcta accagccaga tgactttgcc atccagcgga ccctcggagg
     9601 aacctttgcg gaactaacgt ccgtctggtg gcaaaaactg accctcaatt ccggtgttcg
     9661 agccgatatc tcacaagggt ttcagccgaa gattaccccc agaattggag cgaaatatca
     9721 actgttggcc tcaatccaga ttcgtggtgg ctatggaagg gggttcaaat taccaagctt
     9781 atccagttta ggagatccct tgattgggaa cccttcctta caacccgaaa ccagcgtcgg
     9841 gtgggatctc ggcctagact atcacacagt cgatgaaaga tttaccgcaa caatagaata
     9901 ctttcacaac cggttcagga accttattga cctcgatccc gacctcctca atcaaggaat
     9961 tattcgcctg gccaatattg atgaggcaga aactcatggt tggaatattg ccctgtccct
    10021 cacgccactc cctgccgttt ctttccaatc tagtctcacc cacctcacca ctcaagtgac
    10081 ggaaacggga gctcaactaa gaaaccgccc aaaatggcgc ggatggcttg gggttacaat
    10141 gaagctttct tcgaccctca atgtcaaaag ccaagtcaca tgggtcagtt ccagttttga
    10201 ttttcaagtt ccaacccaaa ccacacgagt aggaggattt gccaaggcag acatcacggt
    10261 caattaccat ccctttactg catggagttg ctatgcagcc ctcgaaaatg tcaccaactc
    10321 ctcatatgaa cagtttgccg gattcccagc tccgcccttg acctttcgtt taggattgga
    10381 ataccggcca gaattttaag gtcagtaatt atttctcata aggaggcatg ggtgacatca
    10441 tcaaccaagt tttctccatg aacttatccc gaaaacctgc ttaagccagc tcatgttttg
    10501 tccgaaaaag gaaaagacgt tgaaaagagg caggcgaaga tgggataaac atcgcctcct
    10561 accttacatt gcactaggaa tctttcatgc aactcatact tccattagat ccggttcagg
    10621 acgaagagcg ttccacgatt tttgcgagaa ttaaaagctg taaatccttg cccattctgt
    10681 ctcccctggc ctcgaccatt ctcaaaatgt gccaaaatga agaaacgaat gcttcagaat
    10741 tagccacgat cataagccag gatccaggaa tggcggctca aattttattt atggcgaatt
    10801 cggggtatta cggtgggtct cgacacaagg tgaccagcat cggacaggcc gtgaccttgc
    10861 ttggattttc aacgatcgcc aatctggctc tatcgttttg cttttatcgt ttaatcaaag
    10921 acatgaaaga ggtcccaaca tccggaattg accatgtaaa attttggcgg cgatccattc
    10981 tggccagtgt cgcagctcgt gccattggaa cacacttcgc ctttcctgat gtagagctca
    11041 tctttcttgg tgccctgctt caggacatcg gactattagc attcaatgag gtggcttcaa
    11101 cagaaattgc ctccctccag gatcacaatc aggacaatca tgccaattta cacctatatg
    11161 aacttgaaca cttaggagtt gatcatgccc aggtgggagc ctggctggcc cagcattggc
    11221 aacttcctga agagtttcag gtggccattc tctatagtca tactcctgaa ttatttgaga
    11281 ctccgctgga ctttcaagct ctggtggata ccgttgccct ctccggtatc atcgcggaca
    11341 tctggattaa tcccgatacc gaagcggcca ttgcctcagc ccgtaagaat agtcagggtc
    11401 gcttaactct gcaaccaaca gattgggaac cgattcttgg gcacatgatg acgggaattc
    11461 cacaaattgc cagttttttt cacagccgga ttgggaactt cgaagacatt gcccacatcc
    11521 atcaagtagc cttacaacag ctgagctcca ctgatcccca cttattcagc taaccagggc
    11581 caactcccgc cactctgact cagcctttct tccccactcc ggcatgctca gtcgccagac
    11641 acaattggtt ccgaccatta tcctttgccc gatataacgc acgatctgca cgttcaagcc
    11701 aggagagcac ggtttcacca ggaagtaact ccgccaaacc gatcgatgcc gtgaccggaa
    11761 tggtgatatc ttggtaagga aatccctctc ctctaatggc atccagcaac cgctcgccca
    11821 gtcgttgact cagttcccca tcggctccct gaagcaagac acaaaattct tcccctccat
    11881 atcttgcaat aaaatcggtt ttcctgggaa atgtcgacac cagacgtttc gccacatgct
    11941 gtagcaccac atccccagcc ggatgaccat aggtatcgtt cacatgctta aaatgatcga
    12001 catcaaccat caaaatgcag actgacgtcc cagagaaaaa actcatactg gccgttcgtt
    12061 ctaattgaag atccaatgct ccacggttat acagctgggt tagagcatcc agcgtcattt
    12121 gctttcgcgc gcctcccaat tcctcctcaa caagacgtaa tttttctcca agattttcca
    12181 atcgttgctg ctggcgttct tttcgttcat ccacaagagc actgatgcct tcaaccacag
    12241 ccaataaggc ctgctttaca tcgtgaaagg aaccgtcctc ggttgccgtg cgcaattgaa
    12301 cgagatgatt ggtcactttt tgatcgattt cctgatcctc cacaaacgcg cgccccatga
    12361 tattggtaaa ttcgcaaaga accgttcgca tatcttgaag atttcgtgtc acataggatt
    12421 tttccttttg acgatgtcca ttaacaaatt gcgtgagcgc cccccaatcc cggcgtggac
    12481 tggcctcgcg tttttttgaa tcgtcggcat caatctgagc gcccaccaac acatgtctcg
    12541 cccaccgttc aaactccttt tctatatcct cttccgagag ttgatcgagt tcaaacgcat
    12601 gacggccgag acatcgcaaa accctggcta aggattccca ggccttgtcg gactctcccg
    12661 agatctctgc agcccgagac ccgttcgcga ccggctcttt ttgtgcccca ttctcatgat
    12721 ggtgatcttt tttcccaatg gaccaagatc ctaacatgtc ttcctcctct tcaactcact
    12781 tctgcctctg tctgtgtcgg tgaccatcca gcgaatgcat ggggagagtg gggtagcggt
    12841 ttgtttccgt tatcggcatt tcctctaggt ctcttgagtc actttttgtt atgctcttgg
    12901 ccaagagcca caaaacacca tgcctaaatg aattccttga tattccattc catcgcccat
    12961 gcaatttttt tctaaaccct tgtgaatcaa cgacacacga tctccagcag acaaaattcc
    13021 catttcaaac gatgggtatc gttacttgac tctcatggga ttaagcgtca tcgcctctgc
    13081 ctcgtttctg ggaataagct ccggagagaa atcgggacgc attctggggt gactgtccac
    13141 gaaatcttac ttcccccctc gtggacctcc agagaggacc tcccttctca agcccttttc
    13201 tatgaactgt cccattccct gtttcaggag ttggacgtgt ttgggaccaa agaaccgctc
    13261 ctggtctgcg gtgtccccga aatgtcacgg agagatctca gccgggcccc caacggattg
    13321 gaagtcctgt gcccgattgg agaccctggg aaccttggag ccttactccg gtgctgccgg
    13381 gcctttgacg tgcaaacagt cgtgttgctt caggaagccg tacacccctt ccacccgaag
    13441 gtcattcgtg ccagtagcgg ggcggtgttt gtccagtcct tggaatgggc aggctccatt
    13501 accgacctga atgccccgga aacattgcaa tggattaccg cattagattt acacgggaca
    13561 aatatgtcca cggtgcaatg gtctaaacat gttcgactcc tcattggaga agaaggcgta
    13621 ggtattccac cctttcagtt tttggaacgc ctgtgtattc ctcaaatcca tccagcgatc
    13681 ccattaaatg caacagttgc aggcagcatt gccctctatg cgtatcgcca acagcactca
    13741 caggtttaac ccattgcgat ccgaccaact acgagacact ccctagccgg tggtagcatc
    13801 acacaaacag agcaataccc acacttttta tgcacacacc acagccgccc caagatcaag
    13861 gccttacgat gattcaaccc ggtagatacc gtcactataa agggaaagaa taccaggtta
    13921 ttggcgtcgc caaacattcc gaaacagaag aagatctcgt ggtttatcgg gcactctatg
    13981 gcgaatccgg cctttgggtc agaccggcaa agatgtttag ggaaaaaatt gaaatagagg
    14041 gaaagctcgt cttcagattt gaatggatcg gcggtgccaa cggtgaacca gacaccacga
    14101 ccatcaaccc gtgaggagtg taaacggaat ttgtcgaatc agacagactg tctttccatg
    14161 gtgattgcca gcccggccag acagctcatc gttgatttcg cttggggggc tcctgggaat
    14221 tctcccatca aagaccgtgc ctgtcatgca ccttatttca aaagctatct gtagaattct
    14281 ccatcgacac tcacaattgt tccgccaaca cagcacgcgt ttgttcttct cgataggcat
    14341 tcaaccgttt ggagagagaa gcatcggtta aggctaaaat cgcagcagcc aacaacgcgg
    14401 cattcacggc tcccgcgctc cctatcgcca aggtccctac aggaatacct ctgggcattt
    14461 gcacaataga caagagggaa tcgaggcctt gcagagccat tgattgcagg gggacaccca
    14521 acaccggcaa ttgggttgtg gccgcaatga ccccggctaa attggccgcc cctccagccc
    14581 cggcaataaa cacctgcacg ccaccggctt ccgccgcacg aacatactcc cccagcgcat
    14641 ctgaagccct atgcgcagaa aggacctgac tttcatgggt tatagccagt ttagtcagcg
    14701 tctcaccggc taaccgcata gtgtcccaat ccgatttgct gcccatgaga attgctaccc
    14761 gaaatgatga ctcagccata gcctcttgta ctccttctta ccttatctat ctgtagatga
    14821 cgagggatga gcgaaaagtc catggttcca gtaaacgggc ctacgatagc gcatcttgac
    14881 ccaagttgac catataacag gaactgtctt aaactgaaat ccctctgttt tttgtaggat
    14941 tttcgttagg agccaacaaa ttttctcgcg ggattttata tgccggagag atgtattatt
    15001 cacctcattc ataatgggag agaaagatta tggcaatacg tctgggagat gaagcaccaa
    15061 attttacagc agaaacgaca caaggtactg tgaatttcca tgaatggctc ggtgacggat
    15121 ggggtatact cttttcacac cctaaggact tcacccccgt gtgcaccacg gaattgggaa
    15181 ccatggcgaa catgacggaa gagtttaaaa aacgaaatgt gaaagttttg gctatcagcg
    15241 tagatccgct cgactcccat aaaggttgga tcaaagacat taacgaaact caacactgca
    15301 cagtgagtta tcccattatt gcagaccccg aaaaaaaggt cgcgaccctc tacgatatga
    15361 ttcatcccaa tgccctcgat aatatgaccg ttcgctcggt gttcgtgatc ggaccggata
    15421 aaaaggtcaa attaaccctc acctatcccg catcaactgg caggaatttc aacgaaattc
    15481 ttcgtgtaat tgactcactg caattaacgg ccaaacataa agttgccaca ccggcaaact
    15541 ggaaagacgg agaggattgt attatcacgc cagccgtgac ggatgcagaa attcccgcac
    15601 tgttcccgaa aggtcatcgt gcggtgaaac cctatcttcg cttcaccccg caacccaacc
    15661 gatagagcat ctgaaaaaaa tatgaggcag cgccacacat gcgtggcgct gcctgtgatg
    15721 catcccatct tcgtctccac cctccctcgc aattgctgat gtattcaggt tctcacatgg
    15781 tttctatggc ctcatacccc atcgaccacg actcattctc gcattaaaag gggatacctt
    15841 cagaattgca agtgtcccag gacgacacaa agccagggga acaagtgcac tcaagttctg
    15901 cacgaggggg gagagaccgc tgacaaaaaa aattaaattt ccccatcagg actcgcttcc
    15961 gttcaaccct tctcagaaga cgctccaaaa
//
LOCUS       DATIAL010000187         3975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_464565, whole genome shotgun sequence.
ACCESSION   DATIAL010000187 DATIAL010000000
VERSION     DATIAL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464565"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLA60_05945"
     CDS             <1..216
                     /locus_tag="VLA60_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08936.1"
                     /translation="GNVKECKMILKYESDILDDENARGNFYHVCQGKVQDTPVGIPPE
                     DQPYECPACGIDLEQEDFLLAQQRGWS"
     gene            258..473
                     /locus_tag="VLA60_05950"
     CDS             258..473
                     /locus_tag="VLA60_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08937.1"
                     /translation="MHLPRSQGRKTVSVMRGLMMLCPACYDSIHEEKLVDEKNYVADH
                     ETFFDVICPGCEDLNRPLIDDMLGSSE"
     gene            854..1321
                     /locus_tag="VLA60_05955"
     CDS             854..1321
                     /locus_tag="VLA60_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08938.1"
                     /translation="MGGFRAGPLIFAVFGFSSIFLISSPPPTLSCDSLLNPSAFQVTT
                     FTQNTLLPNGMMAIRYPETEQEASPHVSVHRVLMVIPGLQESLPEASDMHYRIVEGDA
                     TIGWITYILSAQALFYGVGEDSLGFPEKIWIDPEEDGLNGNEHINGRELGTEK"
     gene            1705..1965
                     /locus_tag="VLA60_05960"
     CDS             1705..1965
                     /locus_tag="VLA60_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08939.1"
                     /translation="MYQEEESFNLRFSLEANFPDEYEGEEDERAWLAHWEETVKPAIV
                     KSIFRTLEQYPDWVARFRNRGKAATDEIEIVLEKTYTDSSLN"
     gene            1970..3481
                     /locus_tag="VLA60_05965"
     CDS             1970..3481
                     /locus_tag="VLA60_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSF08940.1"
                     /translation="MMFWSFAKRSVIFGIAWACMTGWPLSVYAQNLTYSPTPSNPVGR
                     APQAITAGDFNGDGLSDIATVNGTSDDVSVLLGNGNGTFRSAVSFGVGKIPLAVVAAD
                     MDGDGLLDLVLALSGADQVVVLKGEGTGLFKKLASQGAGKGTTFLAVRDVNGDGWSDV
                     VVVNSGRFGYYPPFDLAVLLNDGQGGLLAPVAYEQDGRDGMFPTGVLVEDLTGDGKPD
                     LAVTWSQPSWRSPNGLVSILKNTGNGKFVLEKELKPGFTLSAIQGADINHNGLVDLAV
                     TSLFSDTVRILLQREGGSFTELDPIKVGFAPVGVVFRDFDGDQEMDMVVVNRDSNSLS
                     ILLGNGAGLFTSAGHFGVGATPSAVVVEDFDQDQFPDLATASTNVDGVSVLLSGDGAI
                     PLPSLSTDVMVFEMESGQEANPSSSHHMMRVSNIGLGLLKIVEVKITGNEGDAFSVAD
                     NTCADVTLHTGDSCTLQVGFAPHEPGTHHAMLTIHDNASGSPRRVVLKGLVKS"
BASE COUNT          917 a          936 c         1097 g         1025 t
ORIGIN      
        1 ggtaacgtga aggagtgtaa gatgatttta aagtatgagt ccgatatctt agatgatgaa
       61 aatgcccgag gaaatttcta tcatgtttgt cagggaaagg tccaggacac gccggtcggc
      121 attccacctg aagatcagcc ctatgaatgc cctgcgtgtg gcatcgatct ggaacaggaa
      181 gactttttgt tagcgcaaca acgagggtgg tcataatgtc ggttgagacc gcacccacct
      241 taggcaggga ggacggtatg catttaccac gtagccaggg aagaaaaacg gtatcggtca
      301 tgcgcggcct catgatgctc tgcccggcat gttacgacag cattcatgaa gaaaaattgg
      361 tggatgaaaa aaactatgtg gcggatcatg aaacattttt tgacgtgatt tgtccaggct
      421 gcgaggattt gaatcgtccc ctcatcgacg atatgctggg cagttcggaa tagccccggt
      481 cacctcttga ccgggcatgc tgaacgggtt cttttccccc ataagaccgg gaattctatc
      541 cggcctcccg actttctcgg gttcgtttat tctaaaagtc cgcatttgtc ttaaggcatc
      601 acgatttttt tttaatgaga tcacctgcgc atgcagttta gaccgcaaaa ttcactgaaa
      661 tttttgtgcc gccactgcag aaactgatct gacggcctat cccatccaat tgttcaatcc
      721 attcgccttt tcagccactt cctgggcatg aacttcctgt ctttgttcct ttcctaaatt
      781 actgtgcaga tgtgaccgat aagaagatga gggttaaaag tcgtcgtgaa gattcccgag
      841 caggaggtgt ccgatgggag gcttcagagc tggtccactg atctttgcgg tctttgggtt
      901 ttcatccatc ttcctgattt cttccccccc tccaacgttg tcgtgtgatt ctttactcaa
      961 tccttcagcc tttcaggtta cgaccttcac tcaaaatact ctccttccca atggaatgat
     1021 ggccattcgg tatccggaaa cggagcagga ggcctctccg catgtcagtg tgcatcgagt
     1081 gctgatggtg attcctggac tacaggaatc tcttcctgaa gcctcggata tgcactatcg
     1141 aatcgttgaa ggggatgcca cgattggatg gataacctac atcctttcgg cccaggcact
     1201 tttctatggt gtgggtgaag attcgttagg ttttcccgaa aagatttgga ttgatcctga
     1261 agaagatggc ttgaatggta atgaacatat caatggcaga gagcttggta cagagaaatg
     1321 agagtgccat ctgctaccta agataggctt ccggactgcc taattcatcg agcaggcgcc
     1381 tggtccacgg ggatctccgc aggatccgca actgccttga tcaacaatgt tcattccact
     1441 gcctgtcccg ccgacggcaa atcccgtcaa ctgtttctct agtctagagg cctgacattc
     1501 cggacattcc gggatggttg agccatggac aagcatctca aattgatgcg aacattcttg
     1561 acacctatag gcaaaaatcg gcatgggttc tggacctcct tttaagtgaa atggtaaatc
     1621 tagtataggc gatcatgtga gatgaggaca atggaggttc gcgaaaggga gtggcctacg
     1681 gtcatgatga agaaggagaa aaagatgtat caggaggagg aatcatttaa tctaaggttt
     1741 tccctggaag ccaacttccc ggatgaatat gagggcgagg aggatgagcg agcatggctg
     1801 gcacattggg aagaaaccgt gaagcctgct attgtaaaaa gtatatttcg cacattggag
     1861 cagtaccctg actgggtggc ccggtttcgc aaccgaggga aggcagctac ggacgaaatt
     1921 gaaattgttt tagaaaaaac ctataccgat tcttcattga attaacccca tgatgttctg
     1981 gtcattcgcc aaacgatctg tgattttcgg catcgcttgg gcgtgtatga ccggatggcc
     2041 tctctccgta tatgcgcaga atctcaccta ttcccccacg ccgagtaatc cggttgggcg
     2101 tgctccgcaa gccattaccg cgggagattt caatggggac ggattgtccg atatcgcgac
     2161 ggtaaacggc acatccgacg acgtctcagt gctattaggc aacggcaatg gcacctttcg
     2221 atcggctgtg tcctttgggg tcgggaaaat tccgctcgcg gtcgttgccg ccgatatgga
     2281 tggtgatggc ctccttgatc tggttctggc cttaagcgga gctgatcagg tggttgtgct
     2341 gaaaggtgag ggcaccggcc tgtttaagaa gttagccagc cagggggcag gcaaagggac
     2401 gacctttcta gcggttcggg atgtaaacgg ggatgggtgg agtgatgtgg tggtggtgaa
     2461 tagtggacgc tttgggtatt acccgccgtt tgatctcgcg gtgctcttaa atgatggcca
     2521 gggaggcctg ctggccccag tggcttacga acaggacgga cgggacggca tgtttccgac
     2581 cggggtgttg gtcgaggatc tgaccggtga tggcaagccc gaccttgcgg tgacatggag
     2641 tcagccgagt tggcggtccc cgaacgggct tgtttccatt ctgaagaata cgggtaatgg
     2701 gaagtttgtc cttgagaagg aactcaagcc gggcttcaca ttaagcgcga ttcagggggc
     2761 cgacattaac cataatggat tggtggacct cgcggtgacc agtctctttt cggataccgt
     2821 tcgcattctg ctccagcgcg aagggggatc gtttaccgaa ttggatccca ttaaagtggg
     2881 ttttgcgccg gtgggagtgg tgtttcgtga tttcgacgga gatcaagaga tggacatggt
     2941 tgtggtcaat cgtgattcca atagtttgtc gattttattg gggaatggag ccggactttt
     3001 tacctccgca ggtcattttg gtgtcggagc cacgccctca gcagtggtag tggaggattt
     3061 tgatcaagat caatttcccg atctggccac ggccagcacg aatgtcgatg gcgtgtcggt
     3121 cctgcttagc ggggatgggg ctatccccct cccaagtctg agcacggatg tcatggtctt
     3181 tgagatggag tcgggtcagg aggcgaatcc ttcatcctct catcacatga tgcgcgtctc
     3241 caacatcggg ttggggctgc tcaaaatcgt ggaggtgaag attaccggca atgaaggaga
     3301 cgccttttct gttgcggata acacctgcgc cgatgtgacc ttgcatacag gcgattcgtg
     3361 cacgctccag gtcggattcg ctccacacga accgggaacc caccatgcca tgctcacgat
     3421 tcacgataat gccagcggta gtccgcgccg cgttgtgtta aaaggcctgg tgaaaagtta
     3481 agcaggcgcg ttcattggga tgcatgtata gctgaatcag cccctcttcc cctcccttcc
     3541 catcacgcca ggtgatttct gcgcttcaaa ccttccccat aatccggtat gctctctttc
     3601 cattccttga atggtctgtt gaatatttca aacaccttgc tgtttcatca gcatatgaat
     3661 acccaaggct caccagaaaa aatagtggag tgttcaaaaa ggctcgtcca gtcctgccct
     3721 gaggcatgtc gaaggggagg ccgcagccaa ttggacgagc cgggcgtacg gaggagtacg
     3781 tgagcacggc caggcggcga gaacgtcgct ggcggcattt ttcaacactc ccctgaagaa
     3841 gagtgtgttt gatgtcgact tccggtcaat ccagtcaaaa tgtgtgggta gggatagata
     3901 ttggggggac ctttaccgac tttgtgatct attcctcctc tgatcaatct cttcagcgat
     3961 ttaaaattct ttcga
//
LOCUS       DATIAL010000188         3675 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_787404, whole genome shotgun sequence.
ACCESSION   DATIAL010000188 DATIAL010000000
VERSION     DATIAL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787404"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VLA60_05970"
     CDS             complement(<1..302)
                     /locus_tag="VLA60_05970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08941.1"
                     /translation="MTESNHVDPLLSALRDDSEALRNHAASQLGAVGEEAIPKLINMF
                     LEDDCVIREAATSALVQMGEPAVDPLIEALKDDEEWAVREQAATALGKLRAIKSVAP"
     gene            complement(306..1400)
                     /locus_tag="VLA60_05975"
     CDS             complement(306..1400)
                     /locus_tag="VLA60_05975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08942.1"
                     /translation="MPKETFETIISELDHEEDWRRMRATSTCMKGGPKAVEAIIQAMK
                     TGSSHYKVEAVKMLARLRDPRAGLALAHVLKDEEEQVRQAAVEALEHMAGILDEASAA
                     ALLEYLSDDTLQERMTALLGVIPTSIGPLSERLKDPDPAIRLRAADILAHLLDPRSAD
                     AFVDAMADPDLRDLASDTLRKLGAIRDRVDQIMDDLRAVDESELKEGVRQESVIQLHR
                     IGRPIVEILIEYLEDDDWVVREAAADTLGKIGDIRAVEPLMARLRSDKDTGVKEHALK
                     SLGLIGDPRPVELFIEAIPVRPLRILAVEALEKVKDVEVLRPHIELFARLKTDRDGLI
                     SYNSGVILDKLEAAAAQMGMNEEAWAGKES"
     gene            complement(1416..2513)
                     /locus_tag="VLA60_05980"
     CDS             complement(1416..2513)
                     /locus_tag="VLA60_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08943.1"
                     /translation="MTDSVAEHISALDDEDWGVREDAAVALGRLGDPRSVQPLIRTLR
                     DSDRAVREAATAALKALGEPAVLSLGFCLQDTNSQVQESAACILATIANEQVLDPLLS
                     AALSPNWIVRMSAAQALGRIQSPQAIDTLILLLQDKVPAVREEAGRAIQAIGDSSIPK
                     LLEALQDQNWKIRLRAVEALALLQPREAVGPLMTVVLEDSDTAVRQDAVRALGHIGDT
                     RAIPLLLSSLALNTPSLKLAAIEALGQIRSTEAIPTLIALVNSLPTEAYEERMEGCTD
                     PQYKEDLPPLEAAIKALGKIRDSRAIPALIQALQSTLLRPEAAEALTQFGQAAVNPLL
                     KLLKTTKNDNLRRHVLESLSHLGWRPGQIRL"
     gene            complement(2664..3029)
                     /locus_tag="VLA60_05985"
     CDS             complement(2664..3029)
                     /locus_tag="VLA60_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08944.1"
                     /translation="MPDPDTNLLHITTTETLVRFYVLLAQYIDRCLDETTKRSLPEKE
                     FQTHLAETRALVTELLATNRVVKNKAEMEFQRITALCEHFLQSPADPILKENLQGEHQ
                     VLRIKMLALSDLLAVFRSV"
     gene            complement(3022..3393)
                     /locus_tag="VLA60_05990"
     CDS             complement(3022..3393)
                     /locus_tag="VLA60_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF08945.1"
                     /translation="MHIETKVYPEAVVLRLEGRFDFLAMDTFLTALSQAETLPHSGHI
                     ILDLHQLTFIDSMAIGRIVGTRQRLQRESIRLTLAGQTGYVDTALKAIKLEAMIPTVG
                     SVEEGLALPPSNDSPERIDHA"
BASE COUNT          853 a          953 c          985 g          884 t
ORIGIN      
        1 ggggccactg atttgatagc acggagtttc ccgagagccg tggccgcctg ttcacgaaca
       61 gcccattctt catcgtcttt cagcgcctca atcagcgggt caacggccgg ttcccccatt
      121 tggaccaggg cacttgtggc cgcctcacga atgacacaat cgtcttccaa aaacatatta
      181 atgagctttg gaatcgcttc ctcaccaact gctccgagtt gggaagcggc atggttccga
      241 agggcctcac tgtcatcgcg aagcgcggac aataagggat cgacgtgatt actttctgtc
      301 ataggttatg actcctttcc ggcccaggct tcttcattca tgcccatttg tgcagcggcg
      361 gcctccagtt tgtcaaggat aacccctgaa ttataggaaa tcaggccatc ccgatccgtc
      421 tttaatcggg caaataattc aatatgaggt cgcaggactt cgacatcttt cactttttcc
      481 aaagcttcga cagccaggat gcgaagaggg cgaacgggaa tggcctctat aaacaattca
      541 accggccggg ggtcgccgat gagccccaga gatttcaatg catgttcttt cacgcccgtg
      601 tccttatccg accggagcct ggccatgaga ggttccaccg cccggatatc gccgattttg
      661 ccaagcgtgt ccgcagcggc ttctcgaact acccagtcat cgtcttcaag atattcaatc
      721 aggatttcga caatgggtcg accgatacga tgcagttgaa tgactgattc ctgccgcacg
      781 ccttccttta attcggattc gtctacggcc cgcaaatcgt ccattatttg atcgacacgg
      841 tctcgaatcg cgcccaattt gcgcaaggta tcagaggcca ggtcacgaag atcgggatcg
      901 gccatggcat caacgaacgc atccgccgat cgaggatcga ggagatgggc gagaatgtct
      961 gcagcccgca aacggatggc cggatcggga tctttaagcc gttccgaaag aggaccgatc
     1021 gaagtgggaa tgacaccgag cagagccgtc atgcgttcct ggagggtatc gtcggataaa
     1081 tattctaaga gtgcggctgc agaggcttcg tctaaaatac cggccatatg ctccagggct
     1141 tccacggccg cctgtcggac ctgttcctcc tcatccttca atacatgagc gagggccaag
     1201 ccggctcgtg gatcccgtag gcgggccaac attttcacgg cttccacctt ataatgagaa
     1261 ctgccggttt tcatcgcctg aataatagct tcgacggcct taggtccacc cttcatgcac
     1321 gtggaggtgg cacgcatccg gcgccaatct tcttcatgat ccagttcgga gatgatcgtt
     1381 tcgaaggttt ctttcggcat cctcagatct ttcagttaga ggcgtatttg tcccggcctc
     1441 cagccaaggt gagaaagaga ttccaacaca tgacggcgga gattgtcatt cttggttgtt
     1501 tttaagagct ttaacagcgg attcaccgcg gcttggccaa actgcgtcaa ggcttcagcg
     1561 gcttccgggc ggaggagggt actctgcagg gcttggataa gcgccgggat agcccgggag
     1621 tcccgaattt tccccaatgc cttgatggcc gcttccaggg gaggaagatc ttctttatat
     1681 tgagggtcgg tgcaaccttc catccgttcc tcgtaggctt ctgtgggcag ggaattaact
     1741 aatgcgatta atgtagggat ggcttcggtg gaacggatct ggccgagggc ttcaatcgcc
     1801 gccaatttga gtgagggggt attcagcgcg agagacgaga gcagcagggg aatcgcccgg
     1861 gtatcgccaa tgtgtcccag tgcccgcacg gcatcctgac gaacggcagt atcggaatct
     1921 tcgagaacca ccgtcatcaa tggtcctacc gcctcccgag gttgtaacag cgctaaagct
     1981 tccaccgcac gtagcctgat tttccaattc tggtcctgca aggcctctaa tagtttggga
     2041 atactggaat cgccgatggc ttgaatggcc cgtccggctt cttctcgcac ggcgggcacc
     2101 ttatcctgca gaagcaagat caaggtatca atggcttgtg ggctctgaat acgaccaaga
     2161 gcttgtgctg ccgacatacg cacaatccag ttgggactca atgccgcaga caacaaggga
     2221 tctaagactt gctcgttggc gatggtggcg agaatacagg cggccgattc ctgcacctga
     2281 gaattggtat cctggaggca aaatcctagc gagagaacgg ccggctcccc aagtgccttt
     2341 agggctgcag tagcggcttc ccgcaccgcc cggtccgaat cacgcagcgt ccgaatcaag
     2401 ggttgcacac ttcggggatc gccgagacgt ccaagagcga ccgccgcatc ttcgcgtaca
     2461 ccccaatctt catcatccag agcggatata tgttcagcga ctgagtcggt catgttgatt
     2521 ccaagtaatt atatcggggt cacgtttgcg ttaaccggcc ctcataccac tagggaaaac
     2581 ccgaagttcg acgttaccac acgatttttt gaggggtcaa tgaggaaggg tgagggttaa
     2641 aaaagatggg tgattggcgg gagctacacc gatcgaaaca cggctaacag gtcgctcaaa
     2701 gctagcatct tgatccttag aacctggtgt tccccttgca gattttcttt gaggatggga
     2761 tcagccggac tttgtaaaaa atgttcacac agggcagtga ttcgttggaa ttccatttcc
     2821 gccttatttt tcaccacacg gttcgtcgcg agaagctctg tcaccagcgc gcgagtctca
     2881 gccaaatggg tttgaaattc tttttccggg agtgagcgtt tggtcgtttc atccaggcat
     2941 cgatcgatgt attgggcaag aagaacataa aacctcacca acgtttcggt ggtggtgatg
     3001 tgcaggagat tcgtgtccgg gtcaggcatg atcaatcctt tcaggtgaat catttgaggg
     3061 gggtaatgct aacccttctt ccacagaacc cacggtggga atcatggctt cgagtttgat
     3121 ggccttgagg gcggtatcga cgtacccggt ttgcccggcc agtgtcaatc gaatcgactc
     3181 acgttggagc cgctgccggg ttccaactat acggccgatc gccatactgt cgataaacgt
     3241 aagctggtgc aagtccagaa taatgtgacc ggaatgggga agtgtttcag cttgggataa
     3301 agcggtgaga aacgtgtcca tggcaagaaa atcaaatcgg ccctcgaggc gcaagactac
     3361 ggcctctgga taaacctttg tctcaatgtg catcgtgcac aaacctcctt ttcaagaaca
     3421 ggcctgtgca tcctgtccat taatcggagt gaatttggaa atgatctctg gatcggaaga
     3481 gtagcgtgct caagcaataa aatccagggg aaaatgaaaa agtttcccgg agaccggaag
     3541 aggtggtttt cctgaatacc ttcaaatcaa ttgtttggtc agttcgtcaa ttcccatcac
     3601 acagcttttg gaggaaaaca atttttgaaa atttttcatc tttgttgacc ggaggttatc
     3661 ttcgtgtctc ctgct
//
LOCUS       DATIAL010000189         3439 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1325729, whole genome shotgun sequence.
ACCESSION   DATIAL010000189 DATIAL010000000
VERSION     DATIAL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325729"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..>3439)
                     /locus_tag="VLA60_05995"
     CDS             complement(415..>3439)
                     /locus_tag="VLA60_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08946.1"
                     /translation="VWDWKWDGNVAKGERESTTPHPKYPTAVNWDDSTRPDILFDPTT
                     GKVAWPVLKPHFGKRVPFSANHSGAPWLEPIHQDENGERTSEPAKPGEQGRWSLCPEG
                     ANVPRLNNIHFIRLPIELTPKQGNQESIVDQDGLIYVLHEEERLTRADNNLKYPAVLR
                     ANVYDCVDTILTSEWDDDDYTNFQSSKINIHPHFFQFDTGASDGVISGMEWEMSVRPF
                     TMFSKPKKAGLPVPMNSKLTKAVKAGAKSITIKMAEGASKYHVNTELLVGADCLQADN
                     DPTTSLKRDRSCSEIARIKDIKGETITFVKPLKHDHPTGDIVTPEFVRYRYWVDADTG
                     TVFWHDHAFGATTWPHGGFGATIVEPFGSTYHDPKNGKLVYSGPVADIHTNEPVGFGV
                     SGSFRELMVSIHDTVPHTVNVIQAGNPPGQPIEVALEAGKTVSFQMPEKIQNAPNPFL
                     NGGTHTTGSGFNFRAEPFALRLNNNPDTSKLFSSAIHGDPDTPLLQSYVGDTIVFRLL
                     HQLMNESHVWTLAGHTFLTERFAGDANRKYSIHVGIAERYDLVTKAGGFQGMPGDYIH
                     FNGRSSHFAEGGWGIIRVHDKKVPDLQVLPMGTNPLGIPPVPSSLCPADAPVKAFNVV
                     SLDRPLKLHPNAPDAIEVDFERKIEMTVPDGKIFALEEEATKVAGNVMPNPLTLRVNL
                     GDCIKVNLKNKMAKSRASFFAPGLAFDPRDSHGLNVGNNPGDQTVAPGESRTYTFYAH
                     PANKETTSLVWDGGNIILNPRNGLYGAVIVGPKGSQYRDPVTGEDLSQKNAWRADVII
                     DHTLVENAGKQNYRDVALFFQDEDNIIGTSFMPYVQNVAGLSSVNYRAEPDKFREEQG
                     CTLDKMFQPCVVDKPEDPVTPLIEAHAGDAVRIHVIGANSEQNGMFQVEGHEWPIEPY
                     MPGADMISVVEFGGSETLDVFLRQGAGGPANIPGDYIWANARLNYQQAGQWGYLRVLP
                     TGDQRILPLGATMSNAKRVESQPEPKVLPTAMVRP"
BASE COUNT          778 a          981 c          832 g          848 t
ORIGIN      
        1 gaatggggat ttttggggca ataaattggg cagattagga aattccggta atttgtggta
       61 gcggctggag caactgagac tcaaggcatg cgctgccagg aatgatctga actatcgaca
      121 ctttctcgat acttcttggg atttcatctt cacgacaatc cttccttccc tatcttccaa
      181 gatgaatggg ggaattattg aaggtgtcac tcaaattttc ctgaatggat ttcaatcctt
      241 atgcactgta aaaagaggac atgtcaatcg aagaagaata cgctgaatat ctcaatccaa
      301 ccctggctct atttgatgaa ttcagacatg tcctctttct caaatgagcg ggggagagcc
      361 gcatcatttg tgcagctccc cccgcaaatt cgaccgttct tcccttaacg atgattaggg
      421 ccttaccatc gcggttggca gaacttttgg ctcaggttga gattctaccc gcttggcgtt
      481 cgacatcgtc gctcccaaag gaagaattct ctgatcacct gtcggcagga ctcgcaaata
      541 tccccactga ccagcctgct gatagttcag acgggcattg gcccaaatat aatccccggg
      601 aatgtttgca ggtccgcccg ccccttggcg gaggaacaca tctagggtct cagatccacc
      661 gaattccacc acgctgatca tatcggcacc cggcatgtag ggttcgatcg gccattcgtg
      721 gccttcgacc tgaaacatgc cgttttgctc gctatttgcc ccgattacgt gtatccgtac
      781 cgcatcaccg gcatgcgctt cgatcaaggg ggtaactgga tcttccggct tatcgaccac
      841 acagggttgg aacatcttat ccaacgtaca gccctgctcc tctctgaatt tatccggttc
      901 cgcacgatag ttcacagagg atagaccggc cacgttctgt acataaggca taaacgaggt
      961 accgataatg ttgtcctcat cctggaaaaa cagagcgaca tcgcgatagt tctgcttgcc
     1021 ggcgttctcg accaacgtat gatcgataat cacgtccgcc cgccaggcgt tcttctgcga
     1081 aagatcttcg cctgtcacag gatcccggta ctgagaaccc tttggcccga cgatcaccgc
     1141 gccatacaat ccgtttcgcg gattgagaat gatattcccg ccatcccaca ccaacgacgt
     1201 ggtttcctta ttggctggat gggcatagaa ggtgtacgtc cggctttcac ctggcgccac
     1261 cgtttgatcg ccgggattat tccccacgtt gagtccatga gaatcccttg gatcgaatgc
     1321 cagaccaggg gcaaagaagg acgcccggct cttagccatc ttgtttttca gattcacctt
     1381 gatgcaatcg ccaagattca cacgtaaggt cagtggattc ggcattacgt tgcccgccac
     1441 cttcgtggct tcctcttcga gcgcaaagat ctttccgtcg ggcacggtca tttcaatctt
     1501 tcgctcaaag tccacttcaa tcgcatccgg ggcgttggga tgcaatttca acggtcgatc
     1561 caaggatacg acgttgaaag ccttcactgg cgcatccgct gggcacaacg aactcggcac
     1621 cggaggaatt ccgagcggat tcgttcccat cggaagtacc tggagatcag gtaccttttt
     1681 gtcatgcacc cgaatgattc cccacccacc ctcggcgaag tgtgaactcc gtccgttgaa
     1741 gtgaatgtaa tcaccgggca tgccttggaa tccgccggcc ttcgtcacaa gatcataccg
     1801 ctcggcgatc cccacatgga ttgagtattt ccggttggca tcacccgcaa accgctccgt
     1861 caggaaggtg tggcctgcca gtgtccagac atgcgactcg ttcatcaact gatgcaacaa
     1921 ccggaacaca atggtgtctc cgacataact ttgaagcaac ggtgtatccg gatcgccatg
     1981 aatcgcgctg ctgaacaact tcgatgtatc gggattattg ttcaatctca acgcaaatgg
     2041 ctcagcccgg aagttaaatc cgctcccggt ggtatgcgtt cccccgttca agaagggatt
     2101 gggggcattt tggatttttt caggcatttg gaacgacacc gttttcccgg cttccaacgc
     2161 cacctcaatg ggctgccctg gaggattccc cgcctgaatg acgttgaccg tatggggcac
     2221 tgtgtcatgg atggatacca ttaactcacg gaaactcccg ctgaccccaa accccaccgg
     2281 ttcattggta tgaatgtccg caaccggacc gctatatact aatttcccgt tcttcgggtc
     2341 atgataggta gaaccgaacg gctccacaat cgtggcaccg aacccgccgt gcggccaggt
     2401 ggtcgccccg aacgcatgat catgccagaa caccgttccg gtatccgcat ccacccaata
     2461 ccggtaccgg acaaattccg gcgtcacaat atctccggtc gggtgatcgt gcttgagtgg
     2521 tttcacaaag gtgatcgtct cgcccttaat atccttgatc cgggcgattt ccgaacaact
     2581 ccgatcacgc ttcagcgacg tcgtcggatc gttgtcggct tgcaaacaat ccgcacccac
     2641 caacaactcg gtattgacat gatatttgct ggcgccttca gccatcttga tagtgatgga
     2701 cttggcccct gccttgactg ccttggtcaa cttgctattc atcggaaccg gtaaaccggc
     2761 cttcttcggc ttcgagaaca tggtaaacgg cctgacggac atttcccatt ccatccccga
     2821 gatcacaccg tctgaagccc cggtgtcgaa ctgaaagaaa tgaggatgaa tattaatctt
     2881 cgacgactga aagttggtgt aatcatcgtc atcccattcg cttgtcaaaa tggtatcgac
     2941 gcaatcatag acgttggcac gaagaaccgc cgggtatttc agattgttgt cggctctcgt
     3001 caaacgctct tcttcgtgaa ggacatagat cagaccgtct tggtcgacga tcgattcctg
     3061 gttcccctgt ttcggcgtca gttcaatggg aagccgaatg aagtgaatat tatttaatct
     3121 cggaacattg gcgccttccg gacagagact ccagcgccct tgctcaccag gcttggcggg
     3181 ttccgacgtc cgctctccgt tttcatcctg gtggatcggc tccagccatg gagcgccgct
     3241 gtggttagca gagaatggca cacgcttgcc aaaatgaggc ttcaagaccg gccaggcgac
     3301 tttacccgtc gtcgggtcaa agagaatatc gggccgggta ctgtcatccc aattcacagc
     3361 ggtcggatat ttcggatgcg gagtcgtact ttcccgctcc cctttcgcca cattcccgtc
     3421 ccatttccag tcccacaca
//
LOCUS       DATIAL010000190         7177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1540965, whole genome shotgun sequence.
ACCESSION   DATIAL010000190 DATIAL010000000
VERSION     DATIAL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540965"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VLA60_06000"
     CDS             complement(<1..430)
                     /locus_tag="VLA60_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08947.1"
                     /translation="MTGCLWIGNGAFSGDEKFFKLPQWITGMSTQFKKIIGYLLIVFL
                     VIGFVVLYRYVQPAGYHLPDDPLALEALELVKKHRSRQGYTLDEAVQLMVDDLKTKGI
                     PFHEGDWQVAAQGEDKYMVRKIVREKGSVEWIEREYAWRVD"
     gene            complement(564..1691)
                     /gene="cobD"
                     /locus_tag="VLA60_06005"
     CDS             complement(564..1691)
                     /gene="cobD"
                     /locus_tag="VLA60_06005"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HSF08948.1"
                     /translation="MVTPPVVHGGQIHQVAKALGRSVDSFIDSSASINPFGPPASVLS
                     AMQQALAACQHYPDPSAEELRMRLAKEHGISSDSIVLGNGSSELIRIIPLALSLRQGY
                     VAGPTFMEYEASLHMAGARCTYVLATSAEKYAPPLQQLSLLMDAIRSGYQKEAFGSAE
                     SPAAVFICNPNSPTGRVATARSLRMLCRQIEQAGLWMVVDEAFIDFCPSHSLIKEISN
                     ANRLLILRSFTKFYGMPGIRLGYLVGAPETVATIRGLLPPWSVSHFAQEAGVAAVNDV
                     KYRLRSVKFMQQERQRFMTRLRGIPGLRIIPASANFVMVELSSKCLTASLVSRLAQRG
                     ILVRDCQTFSGMTQPALRLAIRYPRDNNRVIHAIKEALRDS"
     gene            complement(1685..2641)
                     /gene="cbiB"
                     /locus_tag="VLA60_06010"
     CDS             complement(1685..2641)
                     /gene="cbiB"
                     /locus_tag="VLA60_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HSF08949.1"
                     /translation="MADDLTGFKFLAVCALDLMVGDPRWLPHPVRLMGFIIHAYERLT
                     LERISSPWTKRTAGLGLAVGLPLGAFFVTRGILEWAELVHEQFGMVMWVVLGSTTLAG
                     RDLWDHAMRVHRALRIGALVSARVEVGRLVGRDTAALSEEEIVRATVESVSENTSDGI
                     VAPLIYLALGGPACAMAYKAISTLDSMVGYRTDRYRDFGWASARADDLFNWVPARLTA
                     VAMSIAAAIRLGTGVSAWQICWRDAPRHPSPNSGWPEAAMAGALGVQLGGGNVYGGVL
                     ELRARLGDPITPYSMALIPIALQVMGTAYGILVMGIMGWLMW"
     gene            complement(2888..3664)
                     /locus_tag="VLA60_06015"
     CDS             complement(2888..3664)
                     /locus_tag="VLA60_06015"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HSF08950.1"
                     /translation="MRSLWASFSLAWQLLTIIPLPGGSELKIPSATFGSSLRWFPLIG
                     FLLGASLVMIDRLLGSFFPHVLWNMVILALYVLVSGGLHLDGWADTVDALSGGRDPDH
                     RLMILRDSRIGALGATGLILILGLRYAGLLALPVGFREGMLFCMPAIGRWAMVIGCWG
                     VVYPRSEGLAAQFIRTVTWRDVLVATTIVGLGLWGMFDALTAVMLVLVVCLVVRSVVW
                     WISKKFGGITGDVLGAMNEGIEVLFLILGPVLFVFSKFGE"
     gene            complement(3661..4749)
                     /gene="cobT"
                     /locus_tag="VLA60_06020"
     CDS             complement(3661..4749)
                     /gene="cobT"
                     /locus_tag="VLA60_06020"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HSF08951.1"
                     /translation="MNHSSPFIQETLAAIQPVSQAGMCRAQVLWDRLTKPQGSLGRLE
                     SLGTQYVAITQALPVKNPDAMVFVLAADHGVAREGVSAYPSSVTAQMVKNFLQGGAAI
                     NVLARQIGAQVRVVDMGVQEDMGAPPGLWVRKIGLGTRNMCEGAAMSREQAVQSVEDG
                     IRLVQEAYADGIRLIGIGEMGIGNTTVSSAITAVMTRHPVADVTGKGTGVDALQLAKK
                     IQVIERGIQRNTPDVQDPLDVLAKVGGFEIGGLVGILVGGAACRVPVVLDGFITGAAA
                     LLAVALEPQCQAYMIPSHVSAEPGHRLAMDALGFRPLLDLDLRLGEGTGACLAIGLLQ
                     SSLACLTQMATFESAGVDEAERPFPEMA"
     gene            complement(4753..5370)
                     /gene="cobU"
                     /locus_tag="VLA60_06025"
     CDS             complement(4753..5370)
                     /gene="cobU"
                     /locus_tag="VLA60_06025"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HSF08952.1"
                     /translation="MRSIRQSHQRSRRDLFSSPVAVPGPRLSQKKTHVPSKLIFVLGG
                     ARSGKSSFALQQGRGKSPRAFVATGEPFDQEMAGRIQKHQRSRGRGWSTIEIPTRISE
                     WLVEEGSDYPSIVVDCLTLWLNNLLREKVRPSQVPTHVRAFLRSARACPGQVVVVSNE
                     LGLGLVPADAISRSFRDVAGRMNQLVAAEADEVYFLVSGLPLRLK"
     gene            complement(5458..6048)
                     /locus_tag="VLA60_06030"
     CDS             complement(5458..6048)
                     /locus_tag="VLA60_06030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSF08953.1"
                     /translation="MRISKVYTRTGDGGKTRLAGGQEVWKDSVRVEAYGTVDELNSML
                     GLARVSNGQTETNAEVSERMGNILRWTQNKLFDLGGILATAPGESFPNMPTVTPEDVA
                     HLEHLIDECQKDLAPLKEFILPGGGQLAALLHVARTICRRAERLCVTLSKEESIDKEL
                     IIFLNRLSDALFVFARWVTKKQGEAEFLWEREPATS"
     gene            complement(6159..6830)
                     /locus_tag="VLA60_06035"
     CDS             complement(6159..6830)
                     /locus_tag="VLA60_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HSF08954.1"
                     /translation="MTSPLQIDLQPHCLLIRFPGIFNCLSWAPWNGGETSASVVANVQ
                     VGLNRSFPSATMAEDFGVLFKSHSLIPEETIGLMTAANVSAFSDCFLNSSGAWVHVLV
                     TVGLSNARSVLDDADVELGYRGGSVGTVNVVMATNALPTIAGRIQAVHIASSAKAAAF
                     RDNGVTSRKSDRPADLTGTDCLVVGASGEIVEDHCGLHTVLGEMMGRAVHTSVSEGIA
                     EYRRS"
     gene            complement(6880..>7177)
                     /locus_tag="VLA60_06040"
     CDS             complement(6880..>7177)
                     /locus_tag="VLA60_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSF08955.1"
                     /translation="TCCAIQNLWLSARAEGIGVGWVSILDYDSLKKVLDIPQTVWIIA
                     YLCVGYVSGFAHQPDLEKAGWRKRLPLEQLVHYERWGKRSRDESGLSGPGLKAK"
BASE COUNT         1876 a         2165 c         1637 g         1499 t
ORIGIN      
        1 tatccactct ccaggcatat tcccgctcga tccactccac ggaacccttc tcccgcacaa
       61 ttttacgtac catatattta tcctcgccct gtgcggccac ctgccaatct ccttcatgaa
      121 acggaatgcc tttggttttc aaatcatcca ccattaactg aaccgcttcg tccagggtat
      181 agccttgccg ggatcggtgc tttttgacca attccaacgc ttccaatgca agcgggtcat
      241 ctggaaggtg ataccctgcc ggctggacgt agcggtacag cacaacaaat cctatgacca
      301 ggaaaacaat aagaagatat ccgatgatct ttttaaattg agtcgacatg cctgttatcc
      361 attgtggtaa tttgaaaaac ttctcatctc cggagaatgc cccattcccg atccagagac
      421 atccggtcat gtggcgaccg ttttcctatt ggtgttatag cacacatggt tcctacctgc
      481 ctcttccgct taacttttcc ctataacatc catctgttta aacaatcagg tccatccgag
      541 cggatgtctc ccagctcttg atttcagctg tcccgtaacg cttcttttat ggcatgaatc
      601 accctattat tatcacgtgg gtagcgaatg gcaagccgaa gtgccggctg agtcattcca
      661 gaaaatgtct gacaatcccg aacgagaatc ccccgttggg ccagtcgtga tacaagactg
      721 gctgtcaaac atttcgaaga taactccacc atcacaaaat tcgcggatgc gggaataatc
      781 cgcaatccag ggattccgcg caaccgcgtc ataaaccgtt gtcgttcctg ttgcataaac
      841 ttcacacttc gtagtcggta cttgacgtca tttaccgccg cgacaccggc ctcttgcgca
      901 aaatggctga ccgaccacgg agggagcagc ccacggatcg tggcgaccgt ctccggtgcc
      961 ccgaccaaat acccaagacg aattcctggc ataccgtaaa attttgtaaa acttctgaga
     1021 ataagcaatc gattggcgtt cgaaatctct ttgatgaggg aatgggaggg gcaaaaatcg
     1081 ataaaagcct cgtcaaccac catccaaagc ccggcttgtt caatttgccg acagagcatt
     1141 cgaagagacc gggctgtcgc cactcttccc gtcggactat tcggattaca aataaacacg
     1201 gccgcaggtg attccgcgct cccgaacgcc tccttctgat aaccggatcg gatagcgtcc
     1261 atgagaagcg aaagctgttg caggggagga gcatactttt ccgcggacgt ggccaggaca
     1321 tacgtacagc gcgcacccgc catatgaaga gacgcctcat attccataaa ggtaggacct
     1381 gccacatatc cctgccgaag ggatagagcc agaggtatga tccgaatcaa ttcagacgac
     1441 ccgttaccca acacaatcga gtccgatgag attccatgct ctttggccag tcgcatgcgc
     1501 aactcttcag cagaaggatc gggataatgc tggcaggcag ctagggcttg ctgcatcgcg
     1561 ctcaacacgg aggcgggtgg accaaacgga ttaatactgg cactggaatc aataaaagag
     1621 tccacagaac gtccgagcgc ctttgcgacc tgatgaatct gtcctccatg cacaaccggc
     1681 ggggtcacca catcaaccaa cccattatcc ccataaccag tatcccatag gccgtcccca
     1741 tgacttgtaa ggcaatagga atgagtgcca tggaatacgg tgtgattgga tcacctaacc
     1801 tggcgcgcaa ttccaacacc cctccataca cattacctcc accgagttga acgccaagcg
     1861 caccagccat ggccgcttcg ggccaaccac tatttgggct ggggtgccgc ggcgcatccc
     1921 gccaacaaat ttgccaggct gaaacaccgg tacccaatcg gatcgccgcg gcgatgctca
     1981 tggccaccgc tgtaagccga gccgggaccc aattgaagag atcatcggct cgtgcggatg
     2041 cccagccaaa gtcacgatag cgatcggtac gatagccaac catcgagtcc aacgtactga
     2101 tcgctttata ggccatggca caggcaggtc ctcccaacgc gagatagatc agcggcgcca
     2161 cgatgccatc ggaggtattt tcagataccg attccacagt cgcccgaaca atctcttctt
     2221 ctgaaagggc cgcagtgtcc cgccctacta accggccgac ctcaacccga gctgagacga
     2281 gtgccccgat tctcaatgcc cgatggaccc gcatggcatg atcccacaaa tcccgaccgg
     2341 caagggtagt agagcccagc accacccaca tcaccatgcc aaattgttca tgcaccagtt
     2401 cagcccattc caagatgccc cgggtcacaa aaaatgctcc aaggggaagc cccacggcca
     2461 atcccagacc ggctgttctt ttggtccatg ggctcgagat tcgctctaac gtcaggcgtt
     2521 catacgcgtg aatgatgaat cccatcagac ggacgggatg aggcaaccag cggggatcgc
     2581 ctaccatcag atcaagagcg cacaccgcca ggaatttgaa ccccgtcagg tcatccgcca
     2641 ccgttagccc gggaatgttg aaaccgaagg gtcagaggtg tttccggtca attctgcaag
     2701 acaagtcgga caaacgcagt cattatatcg tttcgcgatc cggtcatatt gagcatcgct
     2761 cacgggaaca tctttacacc aacatcgtga gaggccgcac tcaaaggccc caccacaacg
     2821 ttcgcacttc ctagggtagg ttcgtgtcat actgaatcgg cctcccattc atgaatagcc
     2881 cccttaatta ttccccaaac tttgaaaaca cgaagaggac cggacccaag atcagaaaga
     2941 gcacttcgat cccctcgttc atcgctccca gcacatctcc cgtgataccg ccaaactttt
     3001 ttgagatcca ccagactaca gatcgaacca ccaggcacac aactagcacc agcataaccg
     3061 ctgtgagcgc atcgaacatt ccccacaacc ccaaccccac aatcgttgtg gccaccagaa
     3121 catcccgcca cgtcaccgtc cgaataaact gcgcagccaa cccttccgat cgtggataga
     3181 caacacccca gcaaccgatc accatcgccc atcgcccaat agccggcata cagaacaaca
     3241 tgccctctcg aaatcctact ggcagggcca gaagtcctgc atagcgaagc ccaagaatta
     3301 atatcaaccc tgtggcccca agggccccga tacgggaatc ccggagaatc atgagccgat
     3361 ggtcgggatc ccttcccccg gaaagtgcat caaccgtatc cgcccaacca tcaagatgga
     3421 gacctcctga gaccagcaca tagagagcca gaatcaccat attccaaagc acgtgaggaa
     3481 agaaactccc caataatcga tcgatcatca caaggctcgc gccaagcaga aatccgataa
     3541 gaggaaacca gcgaagggag gaaccgaacg tcgcggaagg aatctttaac tccgaacctc
     3601 ctggaagagg aatgattgtc agcagttgcc aagccaaaga aaacgaagcc catagactcc
     3661 tcatgccatt tccggaaacg gtcgttcggc ctcatccacg ccggcacttt cgaacgtggc
     3721 catttgcgtc agacaggcca gactggattg aagcaatccg atagccaaac aggctcctgt
     3781 gccttctccg agccgaagat ccaaatccaa cagcgggcga aagcctaacg catccatagc
     3841 caaacgatgc ccgggttccg cagagacatg ggaaggaatc atataggcct ggcactgggg
     3901 ctccagggcg acggccaaca aggcggcggc cccggtaata aacccatcca ggaccacagg
     3961 cacgcgacat gccgccccac ccacaagaat ccccaccaat ccgccgattt caaatcctcc
     4021 gaccttcgcc agcacatcca aaggatcctg aacgtctgga gtatttcgtt gaatgccccg
     4081 ctcgatcacc tgaatctttt tcgccaattg caacgcatca actcctgtgc ctttcccggt
     4141 cacgtcggcc acaggatgac gcgtcatgac cgcagtaatg gcactgctga cagtggtatt
     4201 tccaatgccc atttctccga ttccaattaa ccggatcccg tcagcgtatg cttcctggac
     4261 aagacggata ccatcttcca cgctttgaac ggcttgttcc cgactcatgg cagcgccttc
     4321 acacatattt ctggtcccca acccgatctt tctcacccac aaaccgggtg gggcacccat
     4381 gtcctcttga acgcccatat ccaccacgcg aacctgggct ccaatctgac gagccagaac
     4441 attgatcgct gccccgcctt gtaaaaagtt tttgaccatt tgtgctgtga ccgatgaggg
     4501 ataggcactg acaccttctc ttgccacacc atgatcggca gccaggacaa ataccatggc
     4561 gtcgggattt tttacgggaa gcgcctgggt gatcgccaca tactgggtcc ctaaggactc
     4621 aagccgacca agactgccct gaggtttcgt cagtcgatcc cataacacct gggcgcgaca
     4681 catccccgcc tgtgagaccg gttgaatcgc cgccagagtt tcctgaatga acggagaaga
     4741 atggttcatc ggtcacttca gtctgagggg caacccactc accagaaaat agacttcatc
     4801 agcttcagcc gcaaccagtt gattcatccg gcccgccaca tctcgaaatg accggctgat
     4861 cgcatcagcg ggcaccaatc cgagacctaa ctcattactg accacaacaa cctgaccggg
     4921 acaggcgcga gccgaccgaa ggaatgccct aacatgtgtt gggacttgcg aaggtcgcac
     4981 cttctcccgc aagagattgt tcagccacaa cgttaaacaa tctacaacaa tactgggata
     5041 gtctgatcct tcctcgacca gccattcgga aatcctggta ggaatttcga tggtggacca
     5101 accacggccc cgcgaccgct gatgtttctg aattcgaccg gccatttcct gatcgaacgg
     5161 ctctcccgtt gcgacgaacg ctcgggggga tttgcccctt ccctgttgta aggcaaaaga
     5221 acttttcccc gaccgcgccc ctccaagcac aaaaatcagc tttgatggca catgagtttt
     5281 tttctgggac aggcgaggac cgggaacagc taccggggaa gagaacaaat ctctccgcga
     5341 acgctgatgt gattgacgga tggaacgcat gaaatcactc cttctcgcag caaactatgc
     5401 aatcatcccg gtatacgctc tgaacacgga taaccgaaag ggccaacgtt acgtctttta
     5461 agaggttgcc ggttcccgtt cccacaggaa ttcggcttct ccctgttttt tcgtgaccca
     5521 acgcgcaaac acaaataacg catcacttag tcgattgaga aaaatgatca attccttgtc
     5581 aatagattct tccttcgaca gggtgacaca caatcgttcg gcacgacgac aaatggttcg
     5641 ggcgacatgc agtaacgcgg cgagttgtcc cccaccaggc aaaataaatt ctttcaaggg
     5701 cgccagatct ttttggcact catcaataag atgttccaag tgggccacat cctccggggt
     5761 cacggttggc atattcggaa acgattctcc cggagctgtg gcgaggatcc cacccagatc
     5821 gaataactta ttttgcgtcc acctcaggat gttccccata cgctcagaca cttcagcatt
     5881 cgtttcggtc tgtccattcg acacccgagc caatccaagc atggagttca attcgtccac
     5941 cgtgccatag gcttccaccc ggacggaatc tttccagact tcctgtcctc cagccaatcg
     6001 ggttttcccc ccatcacctg tacgggtata cactttggat atccgcattg tataagtctc
     6061 cagaaaaaag ttgaataatt cgtttggcct tcagtcgtgc agggactaag tgaaatggcg
     6121 ccaccatcgt atcgttaata atcacacgcc ctcaaggctt aagacctcct gtattccgcg
     6181 attccttctg ataccgacgt atgaacggca cggcccatca tttccccgag aaccgtgtga
     6241 agaccacaat gatcttcgac aatttcaccg ctggcaccta ccaccagaca atcggtaccg
     6301 gttaaatcgg ccggacgatc cgacttgcga ctggtgacac cgttatctcg aaacgctgca
     6361 gctttggctg aggaggcgat atgtaccgcc tggatacgtc cggcaattgt cggaagagca
     6421 ttggtcgcca tcaccacatt cacagttccg accgaaccgc ctcggtaacc cagctcaaca
     6481 tcggcatcgt ctaaaacaga acgggcattg gatagtccga cagtcacgag aacatgcacc
     6541 caggcacccg atgaattcag aaaacagtcg gaaaacgcac tcacgttcgc agcggtcatt
     6601 aatccgatgg tctcttcggg aatgagagaa tgtgatttga acagaacccc aaaatcctcc
     6661 gccatcgtgg ccgaagggaa agatcggtta aggcccactt gtacattggc aaccacggag
     6721 gcggaagttt ccccaccatt ccaaggcgcc caactcagac aattaaaaat tccaggaaac
     6781 cggataagca gacaatgggg ctgaagatca atctgtagtg gagaggtcat tgttttcacg
     6841 gacttatttc cccgcagaat ccaaggatac acagaagtct tatttggctt tgaggccagg
     6901 accggaaagg ccggattcat cacgactgcg ctttccccac ctctcataat gcacgagttg
     6961 ctcaagtggc aatcgtttgc gccatcctgc tttttcaaga tccggttgat gagcaaaacc
     7021 ggacacatag ccgacacaca aataggcaat gatccacacc gtttgaggaa tgtctaagac
     7081 tttttttaaa gaatcgtaat ccagaattga cacccaaccc accccgatac cttcggctcg
     7141 tgccgaaagc caaaggtttt gaatcgcgca gcacgta
//
LOCUS       DATIAL010000191         8583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1002469, whole genome shotgun sequence.
ACCESSION   DATIAL010000191 DATIAL010000000
VERSION     DATIAL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002469"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VLA60_06045"
     CDS             complement(<1..413)
                     /locus_tag="VLA60_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08956.1"
                     /translation="MRCTSVLASNPVSTLYSMLRHPHVGREQLVAFQNERVCRLVAHA
                     YRHVPYYRGLFDRHGLKPQDIRTVEDLGAVPITSRSDLQALPVDEIIARNVDPGSLIT
                     RASSGSSGRPFIVRRTWLEERLHGAFRWRALHALGL"
     gene            complement(410..2299)
                     /gene="asnB"
                     /locus_tag="VLA60_06050"
     CDS             complement(410..2299)
                     /gene="asnB"
                     /locus_tag="VLA60_06050"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HSF08957.1"
                     /translation="MCGLCGVVYSDSKRPVDRDMLQHMTNMMSHRGPDGDGYFVEPGI
                     GLGFRRLSIIDLETGDQPISNEDNSVTVVCNGEIYNYKELRQDLEAAGHRFRTHSDAE
                     VLVHLYEDYGVHCVDFLRGMFGFAIWDSRHRRLMLARDRFGIKPLSYAIKPGALFFGS
                     ELKSILASGCIDRQVDAGAMKELFAIGFVQAPKTLLCSIRRLPPAHYLLHEKGRIAIQ
                     RYWDLTFPSMGEEGFQRSAQEWAEAVRDKLEESVRLHLRSDVPLGCYLSSGIDSSAMA
                     GLMSREISDPIHTFSAAFEDPLFNEIGPNRILTDFSGYNLRNHITTCRTADFDLLPDM
                     IWHREDPNISSGGIPHMLLARMAAQQVKVVLTGEGSDEIFGGYPWYRVERLLEPFINL
                     PLSVRQLIANIPFLKKKYSRSSRAFAAHATMDLSRYTQIIDNASHRSYEDLFSDHLRN
                     IQSSFNDSEPHFLLPKDFEGWNRFAQLQYFDINVRLSDFITRTLDAASMAYGLEARLP
                     FLDHEFVELCSRMPSDLKMRGLQEKHILRRALEGVLPAEILKRKKRGLSAPFWPWQGR
                     LPEFVADALSESRLYTKGYFKPHSVRHLLQQHQNGKANFGRELIGVLNIQLWDDLFVQ
                     GCRPS"
     gene            complement(2544..3689)
                     /locus_tag="VLA60_06055"
     CDS             complement(2544..3689)
                     /locus_tag="VLA60_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08958.1"
                     /translation="MMVEWVISRLSGKAQTLVPRYGFYAWACLGLRGVPVVLLRGPTL
                     QSGHIGTLLVAGHDPWVAYLPNRFFIGEPQRELVGNVSLRALPALLDRLREAADLTIV
                     RADRLSAEKFFGKDYLAVPEWIDMRLALSGDLDELVRSNRSIREDMRLVRKHKLQPLV
                     TEGEERFDQFYDSMYVPFSRERHGAMAIIKGRQELRRFIRKGGILWVMRDNQPLAGVL
                     YERKQDTFNLLAIGMASGEIPLKKRGIMAALYYYSIQHARQLGFAEVNFRAARSSLHD
                     GLLRYKRKWGNALFDNPNSYYDLLIRWNSVNGIVKDFFSHTGLIFRDGGRLSAIHADE
                     SQSRRSLWIEGLHRLYLLTESGPQPMIDEEVLVKRSRSHTRFSSTLS"
     gene            complement(3811..5127)
                     /locus_tag="VLA60_06060"
     CDS             complement(3811..5127)
                     /locus_tag="VLA60_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08959.1"
                     /translation="MDLRSNVTRSPANLRSLQDRLFRAAIIHAYGNVPFYRRVWDEAG
                     VDVQVIRGIQDLDRIPIITAPLVREAAKRGELLARGVDPSSCTYLETSGSSGAALRIW
                     KRPLEERVRRAVGLRIWFEHGFRWRHNTAQFQIKPGPSHVLQRFGISRKTWISTERSI
                     EDQLAQLLEAKADVVVGTATALRRIARASEAAQAALKRPCVVFCAGELVDAETRQLVT
                     RVFGREPVGLYGQTEVGYIAWQCEHRGSFHVNADTHFVEVLRDGIPAHPGELGTIVVT
                     DLRARTMPLIRYNTADLAIAASGGCSCGRQLPLMGSIEGRERASILLGEGRLLTTRKI
                     LDHLAGTLRLGEFRLYQESLKKFRLELTCHALVGHPGIENVDPDSHVHLTVLGHLRKL
                     LGEVDLSIRIVKPWPADGTGKTHAIFRGVNLNQGSLRDRDTTGTAS"
     gene            complement(5158..6555)
                     /locus_tag="VLA60_06065"
     CDS             complement(5158..6555)
                     /locus_tag="VLA60_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08960.1"
                     /translation="MGECGCSAGYAYGQKVLLNAKGYRKRHPALERVMLLDGWQTVRL
                     IRQALASSRWRREDLRQVQEERLRSLLMHAYQNVPLYRGLYTEAGYHPKKFRSLDDID
                     KIPILQKDRLKQASPEEIVAQGIDPQRCGVVETSGSTGTPLRIFLGAIDQQWQRMVAW
                     RILFEHGFRWTDRTLEIRMTFGRQFFVQRLGIAPKEWCSILDPPEFWAQRMVKTRPDV
                     IVAGAGTLQALAETVQTLGLNLPSPRLIVSDSETLSPATRQLVRRALGRDPVDVYGLV
                     ELSNFAWECERRSGFHVSADSHIVEVAAASGEPGPIITTALGMWTMPMIRYETGDLAE
                     VETTLCPCGRTLPLLRRIYGRRVDSVALQDGRRIFWPFFHEVLGRYDELGQWRILQNE
                     INEVHLQIVVTNNVQGLLSRIESDLQAVLPGEIRLRVERVETIPVSPGEKTRMIISKV
                     GNAIEPHPVSGREGL"
     gene            complement(6623..7252)
                     /locus_tag="VLA60_06070"
     CDS             complement(6623..7252)
                     /locus_tag="VLA60_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF08961.1"
                     /translation="MFDELVGEGLTPFLQARAHSVIGMDLSIGNAHLSCRGQTSVCGA
                     CADVRDLPFGNESFDVVVSNSTLDHFQTLDEIIVSLRELHRVLRKGGQLILTLDNPAN
                     PIVAARNVLPFKLLNRLGILPYYVGATCGPWRLQQLLREVGFDVRDVTAVLHCPRILA
                     VLAAKILCSCANSSVQQRFLRWIQVFENMSRWPTRYLTGHFIAVRAERR"
     gene            complement(7985..8293)
                     /locus_tag="VLA60_06075"
     CDS             complement(7985..8293)
                     /locus_tag="VLA60_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08962.1"
                     /translation="METDSKGDEELSRVVAKIQGGVLAVVCGLMGGLGLFIMTVWLLL
                     EGGPKVGSHLQLLSNYFIGYSVTWKGSLVGLFYGALTGGVLGWAIGFIYNRVVGIRGR
                     "
     gene            complement(8328..>8583)
                     /locus_tag="VLA60_06080"
     CDS             complement(8328..>8583)
                     /locus_tag="VLA60_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08963.1"
                     /translation="ILIGTVILVLKGGPVVGPNLSLLGNFLFGFQVTWGGSLIGFLEG
                     GVGGFAIGYSGASLRNWSMKAYAKFIRWREEVNRRRNLLD"
BASE COUNT         2149 a         2420 c         2071 g         1943 t
ORIGIN      
        1 aggcccaacg catgaagcgc acgccaccga aaggccccat gaaggcgttc ctccagccaa
       61 gtgcggcgca caataaatgg tcgcccggag gacccgctgg aggcgcgggt gatcaaagac
      121 cccgggtcca cattccgagc gatgatttca tccaccggca aggcttgcaa atcactcctg
      181 gaggtgatgg gcacggcccc caaatcctcg accgttcgta tatcctgggg tttcagtcca
      241 tgtcgatcaa agagcccacg gtaatagggc acatggcgat aggcatgtgc gaccaaccgg
      301 caaactcgct cgttttgaaa ggccacaagc tgctcccgtc cgacatgcgg atggcgaagc
      361 atggaataga gcgtggacac aggattggag gccaacacac tcgtgcatct cacgacggac
      421 ggcacccttg gacaaatagg tcatcccata attgaatatt gaggacaccg attaactctc
      481 ttccaaaatt cgccttgccg ttttgatgtt gctgaagcag gtgtcgaaca gaatgaggtt
      541 tgaaataccc tttggtgtag agcctgcttt cggacagagc gtccgcgaca aattccggca
      601 aacgaccttg ccatggccag aatggggcgg aaagtccccg tttttttctt ttcagaatct
      661 ccgcgggcaa gacaccttcc agggcccgtc gaagaatatg cttttcctga agccctcgca
      721 ttttcaagtc ggatggcatc cggctgcata attcgacaaa ttcatgatcg aggaatggca
      781 ggcgtgcctc caggccataa gccatggagg cggcatccag ggttctcgta atgaagtccg
      841 aaagacgtac attgatatca aaatactgta attgagcaaa acgattccat ccttcaaaat
      901 ccttcggaag aaggaaatgc ggttctgaat cgttgaaaga actttggatg tttctgaggt
      961 gatcggaaaa aagatcctcg taacttcggt gggaggcatt atcgatgatt tgggtgtatc
     1021 gagacaggtc catcgtggcg tgggcggcga atgcccggct tgatcgagaa tattttttct
     1081 tcaggaacgg gatgtttgcg atgagttgac gcacactgag tggaagattg atgaatggtt
     1141 caagcaaccg ctcaacccgg taccaagggt agcccccgaa tatttcatcc gagccctccc
     1201 cggtcaagac gactttcacc tgctgcgccg ccatcctggc gagcaacata tgagggatgc
     1261 ccccggatga gatattggga tcttcgcgat gccaaatcat atccggcaac agatcaaaat
     1321 cggcggttct gcaggtggtg atgtgattgc ggagattgta gcccgagaaa tctgtgagaa
     1381 tcctattcgg acctatctca ttaaacaacg gatcttcaaa cgccgcagaa aaagtgtgga
     1441 taggatctga gatttctcgg ctcatcaatc ccgccatagc acttgaatcg attccactgc
     1501 tcaaataaca acctaatggc acatcgcttc ggaggtgcag gcgaacggac tcttcaagtt
     1561 tatcccgtac cgcctcggcc cattcctggg cgcttcgttg aaagccttcc tcacccattg
     1621 atggaaaggt gagatcccaa taacgctgga ttgcaatcct ccctttttca tggagaagat
     1681 aatgcgcggg tgggagccgc cgtattgaac acaagagggt cttgggcgct tgaacaaatc
     1741 cgatcgcaaa caactccttc atcgcaccgg catcaacctg acggtcaata cacccgcttg
     1801 caaggataga cttcaactcc gatccaaaaa acagagcacc gggtttgatg gcatagctga
     1861 ggggtttgat accgaaccga tcgcgggcca acatgagacg acgatgacgg gaatcccaga
     1921 tagcgaagcc gaacatacct ctcagaaaat ccacacaatg caccccataa tcctcgtata
     1981 aatggacaag aacttcggca tccgaatgtg tcctgaaacg atgacctgca gcctctagat
     2041 cttgccgcaa ttccttgtag ttgtagatct ctccattgca taccacggtg acggagttat
     2101 cctcgttgga aatgggctga tcgccggtct ccaagtctat gatgctcagt cgacggaagc
     2161 cgagaccgat acccggttca acaaagtacc catccccatc cggtccacga tgggacatca
     2221 tgtttgtcat atgttgaagc atatcccgat cgaccggcct cttagaatcg gaatacacca
     2281 cgccgcacaa tccgcacata gatactccta ctcttgtgtg tcgcgtacct cttagccaac
     2341 cctcggtgaa atgcgtttgg tcggagtcct agttgatgcc tttcaaatca acgactgaat
     2401 acagcgcatt tctatagtca gaaactatcg tctagccaaa ggctaggttt tcgaacctcg
     2461 catctcgcca caacaggtac ccttactgat catcggcgca aaaagcagga atgttgatag
     2521 ggccctcgat gcctccccaa gcatcatgaa agggtcgatg agaatcgggt gtggctccgg
     2581 ctacgcttta cgagaacttc ctcatctatc atcggttggg gaccggactc agtcaggagg
     2641 tagagccgat gtagcccttc aatccagaga gatcggcgac tctgtgattc gtccgcatgg
     2701 attgcagaca agcgtcctcc atcccgaaag ataaggcccg tgtgagagaa gaagtccttc
     2761 actataccgt ttacgctatt ccaacggatc aacagatcat aataggaatt aggattgtca
     2821 aatagggcat ttccccattt gcgtttatag cgaagcaatc cgtcatgcag ggaagaccga
     2881 gcggctctaa aattcacctc agcaaacccc agctggcggg catgctggat gctgtaatag
     2941 tagagcgcgg ccataattcc tctttttttc agtgggattt cacccgaagc catgcctatg
     3001 gccaaaaggt taaatgtgtc ctgttttcgt tcatagagaa caccggcaag tggctgatta
     3061 tctctcataa cccagaggat tccccctttt cgaataaacc gacgtaattc ctgccggcct
     3121 ttgatgatgg ccatggctcc atgacgttct ctcgagaaag ggacgtacat ggagtcatag
     3181 aactggtcaa atcgctcctc cccttcggtg accaatggct gcaacttatg tttgcggacc
     3241 aaacgcatat cttcccggat acttcggtta ctacgcacca actcatcgag atccccagaa
     3301 agggcgaggc gcatgtcaat ccattcagga acagcgagat aatccttccc gaagaatttt
     3361 tctgctgaca atcggtctgc acgaacgata gtcagatcgg ctgcttcacg caggcgatcc
     3421 agcagagccg gcagggcccg taaggacaca tttcctacca gctcacgttg gggttcccca
     3481 atgaaaaacc tgttcggcaa ataggcaacc cagggatcat ggcccgccac caatagtgtc
     3541 ccgatatgcc cggattgaag ggtgggcccc ctcagcaaca caactgggac ccctcgtaaa
     3601 cccaggcacg cccacgcgta aaaaccatat cgcggaacca aggtttgggc cttgccggaa
     3661 aggcgagaga tgacccactc taccatcata ttttttaaat cattcatcct atccgtgccc
     3721 actgtatttg ttccaactgg ccgcaaccac attcgcaagc ctcagctacc ctctttcgtg
     3781 gtttccacga aaccatggga atgtcgcgtt tcaagacgcc gttcccgtgg tgtctctgtc
     3841 tctgagagaa ccctgattga gattgacccc cctgaagatt gcatgggtct tgcccgtgcc
     3901 atctgccggc cacggtttaa cgatcctgat cgatagatct acctcaccaa ggagcttgcg
     3961 gagatgcccc agaacagtta gatggacatg cgaatcgggg tctacgttct cgatccctgg
     4021 atggccgacg agggcgtggc aggtgagctc gagccgaaac ttctttaggc tttcctgata
     4081 aaggcggaat tctccgaggc ggagtgttcc cgcgaggtga tcaagtatct ttcgggtcgt
     4141 aaggagacgc ccctctccga gtaaaattga ggctcgttcg cgcccttcaa ttgaacccat
     4201 caaaggcagt tgccgaccgc aggaacatcc acctgatgca gctatggcaa gatcagccgt
     4261 attgtaccgt atgagtggca tcgtccgggc acgcaaatct gtcaccacga ttgtccctaa
     4321 ttcgcccgga tgggccggta tcccatctcg caatacttca acaaaatgtg tatccgcatt
     4381 cacatggaac gacccacggt gttcgcattg ccaggcgata tacccgacct cggtctggcc
     4441 atacagcccg accggttcac ggccgaagac tcgggtgacc aattgacggg tttcggcatc
     4501 caccaactct cctgcgcaaa acaccacaca tggccgttta agtgcggcct gagccgcttc
     4561 gctcgctcgc gcaatgcggc gaagggctgt cgccgtgcca accaccacat cagcctttgc
     4621 ctccaacaat tgtgccaact ggtcctcgat ggatcgttcc gtcgagatcc acgtcttccg
     4681 agaaataccg aagcgctgca gtacgtgcga gggacccggc tttatctgaa attgggccgt
     4741 gttatggcgc caacgaaatc cgtgttcaaa ccatatccgc agtcccacgg ctcttcgcac
     4801 ccgttcctcg agcggccgtt tccaaatccg tagtgctgca cctgatgagc cgctggtctc
     4861 aagataggtg caggatgagg ggtccactcc ccgcgccagc aactcaccac gttttgccgc
     4921 ttctcgcacc aggggagcgg tgatgattgg aattcgatcc agatcttgaa tgcctcgaat
     4981 gacttgaaca tcgacacccg cttcgtccca aacacgccgg taaaagggga cgttgccgta
     5041 ggcatggatg atagcggcac ggaacaaacg atcttgcaac gatcggagat ttgcgggaga
     5101 gcgtgtgacg ttcgacctca gatccattag gcacttccat gtgcgcaaaa tttccatcta
     5161 gagcccttct cttccggaga caggatgcgg ctcaattgca ttcccgactt tcgagatgat
     5221 catcctggtc ttctctcccg ggcttaccgg gatcgtttcc actcgctcaa cccgcagacg
     5281 gatttcgcca ggcaagacag cttgaagatc cgattctatc ctcgaaagaa gaccttgaac
     5341 gttattggtt acaacgattt ggaggtggac ttcgtttatc tcgttctgca aaattctcca
     5401 ctggcctaac tcgtcataac gccccaggac ctcgtgaaaa aatggccaga agattcgcct
     5461 gccgtcttgc aaagccactg aatctaccct gcgtccatag attcgtctga gaaggggaag
     5521 agtgcgccca cagggacata gtgtggtctc aacctcggcc agatctcctg tttcatagcg
     5581 aatcatcggc atggtccaca tgcctaatgc tgtggtgatg attgggccag gctcccctga
     5641 agccgcagca acctccacaa tgtggctatc agcgcttacg tgaaatccag aacgccgctc
     5701 gcactcccac gcgaaattcg aaagctcaac caacccatac acatcgacag gatcccttcc
     5761 gagcgcacgc cgaaccagtt ggcgagtggc cggcgagagg gtttcactat cggagacgat
     5821 gagtcggggc gagggcagat tgagccccag cgtttggaca gtttccgcca aggcctgcag
     5881 cgtccccgcc ccggcaacga tcacgtccgg cctcgtctta accatgcgtt gtgcccagaa
     5941 ctctggcggg tccagaatcg aacaccactc tttaggagca ataccaagcc gttggacgaa
     6001 aaactgccga ccaaaagtca tgcgaatttc aagcgttcgg tcggtccagc ggaacccatg
     6061 ttcgaaaaga atgcgccagg cgaccatacg ctgccactgc tgatctatgg ctccaagaaa
     6121 aatacgcaac ggagttcctg tcgaaccact cgtttcaacg actccacatc gttgcggatc
     6181 gattccctgt gcaacaattt cttccggact tgcttgcttg agacgatcct tttgaaggat
     6241 agggattttg tctatatcat ccaaggaacg gaatttctta gggtgatacc ctgcctccgt
     6301 gtatagccct cgatagagcg gaacgttctg ataagcatgc atgaggaggc ttcgaagccg
     6361 ctcctcttgt acctgccgta gatcctcacg cctccagcga ctggaagcca gggcttggcg
     6421 aatcaaccga accgtttgcc atccgtccaa caacatcact cgttccagtg ctgggtgtct
     6481 tttccgatat ccttttgcat ttaacaaaac cttttggcca taagcatacc cggctgaaca
     6541 gccacattcg cccatacgtc ccaacttcca agcatcctat tgaaacattc tttcattctc
     6601 gtagggccac cgcaggctgg ctctatctcc tttcagctct gacagcaata aaatgtccgg
     6661 tcagatatcg cgtaggccag cgcgacatgt tttcgaatac ctgtatccaa cgcaaaaatc
     6721 gttgctggac cgaactgttc gcgcaggagc acaaaatttt ggcagcgagc acggcaagaa
     6781 tccgcggaca atgaagcacc gctgtgacat ccctcacgtc gaagcctact tcccgcagca
     6841 actgctgtag acgccaggga ccgcaggtgg cgccaacata gtaaggaagg atacccaacc
     6901 ggtttaatag tttgaaggga agaacattac gtgccgcgac aatcggattg gcaggattat
     6961 ccaatgtcag gattagctga ccgcctttgc gaagcacccg gtgcaattcc cgcaggctca
     7021 cgatgatctc atctaaggtt tgaaaatgat ccagagtgga attcgacacc accacatcaa
     7081 acgactcgtt tccaaacggg aggtcgcgga catccgcaca ggctccacaa acgctcgtct
     7141 ggccccgaca gcttaagtgc gcattcccga tggacagatc cattcctatg acgctatgcg
     7201 ctcgggcctg caggaaaggc gtgaggccct cccccaccaa ttcgtcaaac atgtcagtct
     7261 ttaacaatcg agctactggt tgtgcgggga gccaacgaaa gagaagagat tggttaattg
     7321 aatcgctgtg ttgtcgccaa agccgatccg ggcgggtcat tatccattgg tctgcaatgg
     7381 aatcccagta cctcttccct tcggttatca cgatggcgct ttcatgcctg aaaaaattgg
     7441 gatgtagagc ctttaggtct ggtctacaat tactttcctc cctagccagg cctcgacagt
     7501 gctgtccaga ttgtaacgga ccctccgaac agtgggttgg aaattcaaca cataggcgct
     7561 tcaagcttac acggacaaat ccgcctgagg gtgaaacaga cctccgggta aaaaagtaag
     7621 aagatccact attttgtcct catattaaaa aaggaaaggt ctgatgcggg cgcatgaagt
     7681 tgtgagacac tgacgtggct ggttgcatcc ctgaatggca cctccacacg aattgaatcg
     7741 gcaagatctt cttttgcgaa gtcctctctt atttgttcaa gagcagaggt atacctggcc
     7801 cgtcgaataa aagttcgcac cgcctagccc tccttcagga agttttagcc agatggcttc
     7861 ccacaaaatt gcctgccttc gtgatgatca ttttcatgat ttgttatttt ttggtgttct
     7921 gatctttatc agcgcaaaag cacccatctt atattcaatc aagaaaatcc cagtggcatg
     7981 ccgtttaccg acctcggatt cctactactc tgttatatat gaacccgatg gcccaaccca
     8041 gaaccccacc ggtcagggcc ccataaaaaa gccctaccag gctccctttc cacgtgactg
     8101 aatacccgat aaaatagttg gaaagtaact gaagatggga gccaaccttt ggcccccctt
     8161 cgagaagaag ccagaccgtc ataataaaaa ggcctaatcc acccatcagc ccacaaacca
     8221 ctgccaacac ccctccctga attttggcca caacccggct aagttcttca tcacccttgg
     8281 aatccgtttc catattttcc ctccaggacc ctgcctcttg gtaaaagcta atctagaaga
     8341 ttgcgacgac gattcacttc ctcccgccat cggatgaatt tcgcataggc cttcatgctc
     8401 cagtttcgga gtgaggctcc tgagtatcct atcgcaaatc ccccgactcc tccctctagg
     8461 aaacctataa gtgaaccacc ccacgtcact tgaaaaccaa atagaaaatt ccccagcaga
     8521 gacaagttgg gccccaccac cggcccgcct ttgaggacca aaatcaccgt cccaatcaag
     8581 atg
//
LOCUS       DATIAL010000192         4221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1217967, whole genome shotgun sequence.
ACCESSION   DATIAL010000192 DATIAL010000000
VERSION     DATIAL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217967"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VLA60_06085"
     CDS             <1..1184
                     /locus_tag="VLA60_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF08964.1"
                     /translation="IAGNAVINRLLSLLMIWATALLTYQFRQGMEDRLRLASIVESTH
                     DAIIGETLEGEVTSWNNGAEQMFGYSPQESIGQNMTFLYPPDRIPEHPVIVKKLQRGI
                     PIKNFETVRRRKDGQEIPVSLTISPILDRWGNAIGASKILRDISQQKKLENLLAIQNL
                     SLASHAASLKQSNEDLEQFAYIASHDLQEPLRTIHGFTQLLAERYKDQLDKQGQEFIG
                     FVTDGANRMQTLIQDLLKYSRIQAQELNSIPVQAEEVLNEILKHLRMVIEDKQASITH
                     DPLPIIQTDRSHFQHLLQNLISNAIKFHGPKPPAIHVSAEERSDGWTFSVQDNGIGVG
                     SEYFERIFLPFKRLHTREEYQGTGIGLAICKKIVERRGGRIWVESEAGRGSKFSFTLP
                     R"
     gene            1199..1642
                     /locus_tag="VLA60_06090"
     CDS             1199..1642
                     /locus_tag="VLA60_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08965.1"
                     /translation="MQNPIEILLIEDNPADIRLTQEAFQQARLQNTIHVVQDGVNAMA
                     FIRQTAPFQQAPRPDLILLDLNLPKKDGREVLKEIKSDTHTRTIPVVVLTTSDDEADV
                     LRSYDLHANAYLVKPIDILQFIKMIQTLEDFWLSVVKLPPKVSES"
     gene            1639..3621
                     /locus_tag="VLA60_06095"
     CDS             1639..3621
                     /locus_tag="VLA60_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF08966.1"
                     /translation="MSNLPSPTYSILLVEDNPADAELVRERLKDASGMSPFHITRAST
                     LKEALTILHSKPFDVILLDLNLPETNGLETLKRIRSANSAIPLVVLTVHEDEPLALEA
                     IKLGAQDYLPKSSMNAVLLSRILHYAMEREKNERIRRESERKYRLFVDGATGLAFIML
                     DLLGNITHWNSGAERLFGYTEDAALHKHFSLLFTAEDQRNGRPATELRRAEALEKGDD
                     DNWLVRADGSRFWASGAVTAIRDPNDKLIGFAKVVRDKSAQKDTSDKLAELNRTLEER
                     VIQRTRELTRNQERLRAMASDLTVTEQRERRRLATDLHDYLAQLLVVCRLKLSQGQSM
                     SDKETLLKVIQEADTLLDQSLTYTRTLVSQLSPTCLYEFGLHAALVWLGTEMGKQGLT
                     VNLKCADETPPLPEDQAVLVFQSVRELLYNVLKHSGVNEVMVNLSLQQDNHLLIEVKD
                     FGCGFRENPEERNEQSPTNFGLFSIRERLEALGGEMVLNSAQKKGTQVLLRIPIDHSD
                     EDVQLRAAKPTPVHAVKHASVSRPPSEKIRILLADDHKMVREGFCHILNAQVDFEVVG
                     EAEDGNHALTLCESLRPDVVVMDLHMPKMDGLEAIKRIKEFLPDTVMIGLSVYDTPDV
                     ARWFREAGAAAFVTKGGPAKNLVTIVRKYCQQKNPA"
     gene            complement(3631..>4221)
                     /locus_tag="VLA60_06100"
     CDS             complement(3631..>4221)
                     /locus_tag="VLA60_06100"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSF08967.1"
                     /translation="GTTLDLKGEPIGIVAELDISFRKQEGQEALNVTFASGPGKFSPF
                     TQVAIQSGIVSTARMAGLDPKTWNIFFKFPKPGVTIYGDSLTAMVSVVALAMANDNSV
                     LLNRVMTGKVTRDGHIGAVGGIPLKIQAAFAEHIPRVIIPDDRFPEDNDWETPFLMQI
                     SPVNTVLQAYQMLTGTTLPSPEKASGLMVVHNTNEK"
BASE COUNT         1110 a         1103 c         1013 g          995 t
ORIGIN      
        1 cgattgccgg caatgctgtg atcaatcgtc tcttatcgct tcttatgatt tgggccacag
       61 ccctcctcac ctatcaattt cgtcagggga tggaggaccg gcttcggtta gcctctatcg
      121 tggaatccac ccatgacgcc atcatcggag aaacactcga gggagaggtc accagctgga
      181 acaacggcgc cgaacaaatg ttcggatatt cgccacagga aagtattggg caaaatatga
      241 cgtttcttta ccctccagac cgaattcccg aacatccggt tattgtcaaa aaacttcagc
      301 gtggaatacc gatcaaaaat ttcgaaacgg ttcgccgccg gaaagacgga caggaaatac
      361 cggtatcgct gaccatttct cccattctcg accgatgggg aaacgcgatt ggcgcctcca
      421 aaatcctacg ggatatttcc cagcaaaaga agttggagaa tctcctggct atacaaaacc
      481 tttccttggc cagtcacgca gcctccctta agcaatcaaa cgaggatctc gaacaatttg
      541 cctatatagc ctctcatgat ctccaagaac ccctccgcac gattcatggc tttacgcaat
      601 tgttggcgga acgctataaa gaccaattgg acaaacaggg tcaggaattt attggattcg
      661 tgaccgatgg agcgaatcgc atgcaaaccc ttattcaaga cctcctgaaa tattcaagaa
      721 ttcaggccca ggaactcaat tctattccgg tccaggccga ggaggtgctc aatgaaatcc
      781 tgaaacatct ccggatggtc attgaggaca aacaggcttc gatcacccat gatcctctgc
      841 cgatcattca gaccgatcgg tcgcattttc aacatttgct ccaaaatctg atttccaacg
      901 ccattaaatt tcacggcccg aaaccgccag ctattcatgt atctgcggag gaaaggtccg
      961 acgggtggac cttttcggtg caggataacg ggatcggcgt cggatcggaa tatttcgaac
     1021 ggatctttct tcccttcaaa agactgcaca cgcgggaaga gtaccagggc actggaatcg
     1081 gactggccat atgtaagaaa atcgtagaac gccgtggagg ccgcatctgg gtcgaatccg
     1141 aagcgggaag aggatcaaag ttttctttta ccttacctag ataaaccgga gaatgttcat
     1201 gcaaaatcct attgaaattt tactcataga ggataatccc gctgatatcc ggcttaccca
     1261 ggaggccttt cagcaagctc gcctccagaa tacgattcat gtggttcaag acggggtcaa
     1321 cgccatggcc ttcattcggc agacagcacc ttttcaacag gctccccgac cagaccttat
     1381 tctgttagat ttaaaccttc ccaaaaagga cgggagagag gttctgaagg aaatcaaatc
     1441 cgatacccac actcgcacaa ttcccgtggt ggtgctgacc acctccgatg acgaagccga
     1501 tgtcttgcgg agttacgatc ttcatgccaa tgcgtatctg gtcaaaccca ttgatatttt
     1561 gcaattcatt aaaatgattc aaacactcga agatttttgg ctctctgtgg taaaactccc
     1621 tccaaaagtg tcagaatcat gagcaatctc ccatccccta cgtattccat cctgctcgtg
     1681 gaggacaatc ctgccgacgc agaattagtg cgcgagcggc tgaaggatgc ctccggcatg
     1741 tccccctttc atatcacccg cgcatcgact ctgaaagagg cgctgaccat ccttcacagc
     1801 aagccgtttg acgtcatcct tcttgactta aatctgccgg aaaccaatgg ccttgaaacg
     1861 ttgaaacgaa tccggtcggc aaattcggcc attccccttg ttgtgttaac cgtgcatgag
     1921 gatgaaccgc tggcccttga agccatcaaa ttaggggctc aggactatct ccctaaatct
     1981 tcgatgaatg ccgtgttgct ttcccgaatt ttgcactatg ccatggaacg ggaaaagaat
     2041 gaacggattc gccgggagag cgaaagaaag taccggctat ttgttgatgg cgccaccggc
     2101 ctggcattta tcatgcttga tttgcttggg aacattactc attggaattc cggagctgaa
     2161 cggctttttg ggtacacaga agacgcggca ttacataaac atttttcgtt actctttacc
     2221 gctgaagacc aacgcaatgg tcgtccggca acagaattgc ggagggcgga agccttagaa
     2281 aaaggggatg atgacaattg gctggtacgg gccgatgggt cacgattttg ggccagcggc
     2341 gcagtgacgg ccatacgcga ccccaacgac aagctgatcg gatttgccaa ggtggtccga
     2401 gacaaaagcg ctcaaaaaga taccagtgac aaattagccg aattgaatcg caccttggaa
     2461 gagcgcgtga tccaacgtac tcgggaactc actcgcaacc aggaacggct acgcgccatg
     2521 gcttccgatc tcacggtaac cgaacaacgc gaacgacgcc ggttggcgac ggaccttcat
     2581 gattatctgg ctcagctcct tgtcgtctgt cgactgaaat taagtcaagg tcaatcgatg
     2641 tccgataagg aaaccctcct taaggtgatt caggaggcgg ataccctcct ggaccaatcc
     2701 ttgacctata cacggacact ggtcagccaa ctcagtccga cctgtctcta tgaatttgga
     2761 ctccatgccg ctctcgtctg gttaggaacg gaaatgggaa aacagggatt aacggtcaac
     2821 ttgaaatgtg cagatgagac gccacccctg ccggaggatc aggccgttct cgtgttccaa
     2881 tccgttcggg aactattata taacgtgctc aaacattccg gcgtcaatga ggtgatggtg
     2941 aatttgtctc tgcaacagga caaccatctg ctgatcgagg tcaaggattt tggctgcgga
     3001 tttagagaaa atcccgaaga gagaaatgaa caatccccaa ccaattttgg actctttagt
     3061 attcgggaac gattggaagc cctcggcgga gaaatggttc tcaattcagc ccagaagaag
     3121 ggaactcaag tcctcctgcg tattccaata gaccacagcg atgaggatgt ccagttacgt
     3181 gctgccaaac cgactccggt gcatgccgtt aaacatgcgt ctgtttcacg acccccttcg
     3241 gaaaaaattc gcattcttct cgcggatgat cataagatgg tccgtgaggg gttttgccat
     3301 attttgaacg ctcaagtgga ttttgaagtg gtgggagagg cagaagacgg gaaccatgcc
     3361 cttacccttt gtgagtcact ccgtcccgac gtcgtggtga tggatcttca tatgcccaag
     3421 atggacggtc tggaagccat caaaagaatc aaagaattcc ttcccgacac ggtcatgatc
     3481 ggattatccg tctacgacac gcccgacgtg gccagatggt tccgggaggc aggagcggcg
     3541 gcatttgtca ccaagggagg tccggctaaa aacctcgtca ccatcgttag gaaatattgt
     3601 caacaaaaaa accccgccta gccttcctgc ttatttctcg ttggtattat gcaccaccat
     3661 caatcccgag gccttctcgg ggctaggaag ggtcgttccc gtcaacatct gataggcctg
     3721 caacaccgta ttcacgggag aaatttgcat cagaaaaggc gtctcccaat cattgtcttc
     3781 gggaaaccga tcgtcaggaa tgattactct tgggatgtgt tccgcaaagg cggcctgaat
     3841 tttcagtggg atccctccca ccgccccgat atgaccatca cgagtcactt taccggtcat
     3901 cacacggttc aggagaactg agttatcgtt agccatcgcc agagccacga cactcaccat
     3961 cgcggtcaaa ctatctccat aaatcgtgac accgggtttc ggaaatttga agaatatatt
     4021 ccaggttttt ggatcgagac ctgccatgcg agccgtggag acgataccgg attgaatagc
     4081 cacttgagta aatggtgaaa attttcccgg tccgctcgca aatgtcacat tcagggcttc
     4141 ctgcccttcc tgcttcctaa acgagatgtc aagttcagcc acaattccta tgggctcacc
     4201 tttcaggtca agagtggtcc c
//
LOCUS       DATIAL010000193         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_895529, whole genome shotgun sequence.
ACCESSION   DATIAL010000193 DATIAL010000000
VERSION     DATIAL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895529"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..899
                     /locus_tag="VLA60_06105"
     CDS             372..899
                     /locus_tag="VLA60_06105"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HSF08968.1"
                     /translation="MNHMSNIATVLSSEELSQVWADLAQNDQLPDWYELTEHGELIMS
                     PKPSNRHQVICAEIAYQLRAQLGGKAVSEAAVLTTSAGIRVPDIVWMPEDKWQVVTIE
                     EGLIRAPDFVVEVLSPGNRQVEINYKVQAYLASGIQEVLVVGLKGSLEFYRQDGIHTT
                     SSFNVKLSLPPHLFQ"
     gene            complement(1040..2569)
                     /locus_tag="VLA60_06110"
     CDS             complement(1040..2569)
                     /locus_tag="VLA60_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HSF08969.1"
                     /translation="MALSLSECESIVRELRKALAGGFVQKIHQPRDLTLTLDIRAQGQ
                     TSHLLVCADARFARLHLTSQKFENPQTPPPFCAFLRSHVEGGRIAEISQEPGDRIVFI
                     TIAKAGHLSVLVIALTGNQANVHVLNEKKLVLRSLRDSRVKAGERYTPPTLRPVPSSE
                     ALPTSAMDGRIFQDEIVIQQEEKIREPHSEAATQGTGTFGEIFPVSAGLEARYRQREQ
                     EEGRETILEQQLAQVRKALKHAKRKIHALQEDFKKTERFREYARYGELLKSHLHEIKK
                     GQASIAMVDYYDPALPTLTLPLDPAKDAVWNMEDYFRKYHKYIGAQEHLQPRVDQAQQ
                     EVARLEAKLAQLEQGIVDPEGLPRKKKKPGSSIPQGTGVSNGRPAPAQGYRTYISADG
                     LPILVGKTAKDNDHLTLKVANPDDLWLHARGTPGSHVVVRLEKGAMVPPETLKDAATL
                     TLWFSDLRKSGKGEVIYTLRKFVKKGKGFKPGSVTVEREKSLWIELKEDRLNRLKGLS
                     S"
BASE COUNT          686 a          751 c          696 g          779 t
ORIGIN      
        1 tattccgcac agaaacggga aggcgccgat gtaatagtcg ccgtgggtca aatttcaccc
       61 acaacaccca atatccatga aaaattccta gtctggttcc cctttcaagc ggctcaatct
      121 ctcctctttt aattcattcc agagagtctt ttccctctcc accgtaacgg aaccgggttt
      181 gaagcctttg ccttttttga ggaatttccg caaggtataa atgacttcgc ctttgccgct
      241 ctttcgcaat tcactaaacc agagagtcag cgtcgccgca tcctttaatg tctccggagg
      301 aagcttcaag gtagagaagg gctctattgg cagggttagt ttcattgaca ggatggaagg
      361 agattcctaa aatgaaccat atgtccaata ttgccactgt tctctcatca gaagaattat
      421 cccaagtttg ggctgacctc gcccaaaatg atcagcttcc agattggtat gagctcaccg
      481 aacatggaga actgatcatg agtccaaagc ccagcaaccg tcaccaagtt atttgcgcag
      541 agattgccta tcaactgcgc gcacaacttg gcgggaaagc ggtctctgag gctgcggttc
      601 tcaccacatc agcaggcatt cgggttcctg atattgtctg gatgcctgaa gacaaatggc
      661 aggtcgtgac catagaagaa ggattaattc gtgctccaga tttcgtcgtt gaggtgctat
      721 cacctggaaa tcggcaggtc gaaatcaact ataaagtcca agcctatctt gcttctggca
      781 ttcaggaagt tctcgtggtt gggctcaaag ggtctcttga attctatcgc caagatggga
      841 ttcatacgac ctcttcgttt aacgtcaagc tctcacttcc cccgcacctc tttcaataat
      901 aacaagagaa catcagctta tgaatttctg agcgagcccc caatgggtat cggaagattg
      961 taggtaggcc tcggggcttt ttctttgtgc tccactcctg ttttttattt ccatcacgtg
     1021 agcagattga ggcctgattt taggaagaga gtcctttcaa ccgattcaat cgatcttctt
     1081 ttaattcaat ccagagcgat ttctctcgct ccacggtcac ggaaccgggt ttgaagcctt
     1141 tgcctttttt gacgaatttc cgcaacgtat aaatcacttc gcctttcccg ctttttcgca
     1201 aatcactaaa ccacagggtc agcgtcgccg catcctttaa tgtctccgga ggcaccatgg
     1261 cgcccttctc caagcggaca accacgtgcg aaccgggtgt ccctcgagca tgaagccaga
     1321 ggtcatccgg attggcgacc ttcaatgtca gatgatcatt atccttcgcc gtttttccga
     1381 ctaaaatagg aagtccatcg gcagagatat acgtccggta gccctgggct ggagccggtc
     1441 ggccattgga tactcccgtc ccctggggaa tcgaggaacc tggttttttc ttttttcttg
     1501 gcaaaccctc aggatccaca atcccttgtt ctagctgagc caatttcgcc tccaatcggg
     1561 ccacttcctg ttgggcctgg tccacacggg gctggagatg ttcctgagcc ccgatatatt
     1621 tatgatattt ccgaaagtaa tcttccatgt tccacaccgc atccttagcc gggtcaagcg
     1681 ggagcgtcag ggtgggtaag gcgggatcat aataatcaac catggcgatg ctcgcctgtc
     1741 cctttttgat ttcatggaga tgacttttca acagttctcc ataccgggca tattcccgaa
     1801 accgctcggt ctttttgaaa tcttcttgta acgcatgaat ttttcttttg gcatgtttca
     1861 gtgcttttcg aacctgcgcc agttgctgct caaggatggt ttcccggcct tcctcctgtt
     1921 ctcgctgcct atatcgggct tccagtcccg ccgataccgg aaatatttcg ccaaaggtac
     1981 ctgttccctg agtggcagct tcagaatgcg gttcacgaat tttttcttcc tgctggataa
     2041 cgatctcatc ctgaaagatg cgaccatcca tggcggaggt agggagagct tctgaggagg
     2101 gcaccggtcg caacgttgga ggtgtatagc gctctcccgc cttcacgcgg gaatcccgta
     2161 aggatctcaa gaccagtttt ttctcattta ggacatgcac atttgcctga ttgccggtca
     2221 acgcaattac caaaacggac aagtgcccgg ctttggctat tgtgataaac acaatccggt
     2281 ctcccggctc ttggctgatc tccgcaatgc gtcctccctc cacatgagaa cgcagaaagg
     2341 cacaaaaggg agggggagtc tgcggattct caaacttctg agaggtcaga tgcaggcggg
     2401 caaaccgggc atccgcgcac acaagtaagt ggcttgtctg tccctgggcg cgaatatcga
     2461 gggttaaggt caagtcccgg ggttggtgga ttttctgaac aaacccaccg gccagagcct
     2521 tgcgtaattc cctgacaatg gactcacatt cagaaaggga aagagccata gttgaatttt
     2581 accggatcaa aaaagaggag tcacagcaga tatgaatgtt gtatggttcc aggaagttga
     2641 cggacaatac tcttcccttt tgccaagaga gtattctcgt gaggaagagg tgtaaattaa
     2701 tggtacttcc gagtttagtg tgggcaaacc cctcggccaa tgcttgagac catgagctac
     2761 acatttctcc actttgtaag gggtttcatt taaaagaggg aaaaggtgag gtagagattc
     2821 aggtgtgtgc ccatgtcatt gcgccagcag cccttgcgac aaattggcat ctggcgtttc
     2881 tacctcccct tgcccctcct tacaaaggag gg
//
LOCUS       DATIAL010000194        11521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_788207, whole genome shotgun sequence.
ACCESSION   DATIAL010000194 DATIAL010000000
VERSION     DATIAL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788207"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..877
                     /locus_tag="VLA60_06115"
     CDS             158..877
                     /locus_tag="VLA60_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HSF08970.1"
                     /translation="MTFAANPSEFFGSLGLLSIVILFILSIFSVISWGIIVNRFRRFS
                     RIRQEESQFLQLYEQNPIDQHTLRSQAQTLAYSPSSVVYLGITDRLPESSDLFTSRPP
                     MEAKHTERSPNRQYLEKVAQYIIQNQIGQQEAYLPFLATTGNLTPFIGLLGTVLGIIN
                     AFHEIGLQGSASIAAVAPGVSEALVATAAGLFAAIPAVMAYNYFLSRIRRMSFRVEAF
                     TIEFLNTIEEAEKAFEVEVTR"
     gene            874..1314
                     /locus_tag="VLA60_06120"
     CDS             874..1314
                     /locus_tag="VLA60_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HSF08971.1"
                     /translation="MNTGSKPRQFLSEINVIPLVDVVLVLLVIFMVTAPMLHRGLDIN
                     LPTTTANNIKAEERLIVTIQRDHTLSLGNDTISLVNLRAKLQEAKTLNPLISVYLRAD
                     QTVPYGTVVQVMDEVKGAKIERLGMITGPKIETLIEDETIGISR"
     gene            1446..2822
                     /locus_tag="VLA60_06125"
     CDS             1446..2822
                     /locus_tag="VLA60_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HSF08972.1"
                     /translation="MMALFLRFQSTVEEPLRAIDVTLISVPETQAPAPSQKKSSPPPP
                     PSQQKSQPTKKIEPTKTQPIAPPLPPLPVPKASERLSEFMEGAVGSIVMPHKQEITSP
                     TPPQPINEQPPSNDQSPLFENLRMPPIAPQISRPERLHSSQPLVIPKPTPTPAPSPQE
                     AAPPTTVPPEPQGSESQPQAPKIEPTVKLAPVLPELSSVTPFRASKKERKPNDASPLN
                     NLEESLKRTIPTVPIPAPTPKIKKQHKTITPKQEKPFVPEISPPQLAQISPSPPLEKA
                     PQREKMSDMMKQLLEEARVPNLQSSPPAPSSQQPLPSSSTSTQPVQSEIDLQIAKLSI
                     PNVTPVESIKERLQRLEVQPNSKTGQSSSPSSPGKNQYLAMVEDRIDHEWRALPLVTN
                     PPVVVLKFRISRFGEISNIHIDESSGNGNYDSAAKRAVSAVNPLPPFPPDISDSFFEV
                     RFRFIKKD"
     gene            2841..4199
                     /gene="tolB"
                     /locus_tag="VLA60_06130"
     CDS             2841..4199
                     /gene="tolB"
                     /locus_tag="VLA60_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HSF08973.1"
                     /translation="MKKALNPLYAILALLAFSVISVSPAFLHGEEADLKATRSEFQKI
                     PIWVMGFSPVQRDTGISEDLESQVASVLKADLKRSQIFSIAELPPAKGDFSDNKCMSL
                     SSNLAPHFQKVTVSTWGRIGVGGTSSGVQGLILDACAFDPGQQDVLTGKRYFSLKTTE
                     TLTRLMAHRWADELVYRYTGEQGIARTKIAFVGQKDNGRELFVMDYDGYNPQQVTADG
                     YLNLMPTWAPDRKSLIYTAYRDRKQLIIKRELATGREEVLVSPASLNITATFAPNGKS
                     IAYSAAQDGNSDIYQIELDSRSVKQLTSHNSADLSPSWSPDGRHLAFTSDRGGRPQIY
                     IMDADGLNARRLTYDGDYNAAPAWSPKGDWIVYVCRIPNEGFKLCRISPNGEQRKQIT
                     NGQSIDDSPSWAPNGRHIVFSSIRRGESQLYIITSEGAGLEKISLAGEHLSSPSWSPF
                     QP"
     gene            4244..5197
                     /locus_tag="VLA60_06135"
     CDS             4244..5197
                     /locus_tag="VLA60_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF08974.1"
                     /translation="MRPAIHMISLLGILSLSLISISGCGEKRIHVSTASGSPGEETGE
                     SASGTDTAGLTGTSINETPLSGQGASTDGIRAEAVQPPAPVEPSNFGYDPNHMDESFK
                     QNLQAGIPSGNDSEIEKNDASPSTPFKDGKDILTQSSSSSMNGSPHDQTETSPFSSQT
                     EFQNDLSNLPKDMEPIPETFQVAKAEPSEIIREQLDKIQEEELATVSAGLEDVFFHFD
                     SWSLTAEAKDSLERDMAWLTNDPSSLLIIEGHADQRGTQAYNMVLGKKRAMAVRDFLS
                     QLGVDPSRLTVISYGKDKPFCQDTTEVCYQLNRRGHLLVQN"
     gene            5358..6986
                     /locus_tag="VLA60_06140"
     CDS             5358..6986
                     /locus_tag="VLA60_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08975.1"
                     /translation="MAQQADLARIQKDLEQQIAKIKEEKKTLGAQVDETKAQLIKMNE
                     EAQKTRGNLASINQKATLLQEKDVASLYGKLEETEKSIQDLKKDFTNQTGALKSDVQS
                     IQSTIQTHGEDLQTVKTQNTNLAQQVDENNLALTSNMGEFQNSLTQFKETLTSLGATI
                     GQVQTDLSGQRQDLGTVQSRMEELPRVFNTRLEKQSKQITQLENQVTTLQEKLTADTQ
                     ALRSYLEQDVKASMARLVADIDAHQGPVLAQIDSLQKDMEALGTHVQADASHVQDLSQ
                     SVLKLREAQEVMGSLLGKRGDEIIQQAGRISERMNMIESHQTALTEQLQSNTQKTSAH
                     LAEVNASLTSITQALDQARQSLSSRLAKHEEAGQTLNQSVQQIQQLKRDVQDQKQQAQ
                     AVNQIADQLNQTVERIGSRLKDLETHQSGLVGKIDSDTQMTNTHLQEVNSGIQSVAQA
                     LENVSTKLNARIESQEQRLNRAMTSFQQVQGTADTSQNNLTHLNQLTETVNKLRDVIN
                     TIGTKLGERVDQHEDRLGQLAQRVNRLQSPKPQK"
     gene            7145..8050
                     /gene="ybgF"
                     /locus_tag="VLA60_06145"
     CDS             7145..8050
                     /gene="ybgF"
                     /locus_tag="VLA60_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HSF08976.1"
                     /translation="MTPKFSTLSICIGFALTGGWGCATEPNLTDIQKQVQTLIIQQEA
                     SHKQEQQILDRMETVESQLDEHDFLVGELIKTEEEASLDTRHLLEKLERTSTLLREQI
                     EQTRSTTQRRDQDLSIRVKALESRIDNVIHQPRSVSESPEVTTPNTTSPSSDPKATQT
                     GIPASPTSPGERTGAENEASAFRSAYKVYLNGNYDRASVEFQRFVTNYPSTTLTPQAY
                     YYLGESFYVQKQYDPAKQALEQVISRYPSSKYRSHALYKLGQIMLEIDQRSKAQELWN
                     SIIQDYPDSPESTQAKEQLKKSGLS"
     gene            8109..9899
                     /gene="mutL"
                     /locus_tag="VLA60_06150"
     CDS             8109..9899
                     /gene="mutL"
                     /locus_tag="VLA60_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HSF08977.1"
                     /translation="MLSTVTGKVQILPDSVSCRIAAGEVVERPASVVKELIDNSLDAG
                     STLITVEVEEGGRRVIRVIDNGTGMPRMDAQLACQRFATSKLRSEDDLLTLMTMGFRG
                     EALPSIASIARFCLKTVPRDSTIGTQTQSTGGVAWEVQDYTGPQGTQVEVRDLFFNTP
                     GRLKFLKTVPTEFSKICYVVQQAAAVHPEIHFRLRHQNHKVLEYPAVSSLQDRLLQIY
                     GPDFLERFLPVTYNAGSFQLTGYTISPHHARASRAPQEIFVNGRPIKNTTISHAVLEA
                     YGSFLPKGKHPQFIVFIHLDPQSIDINVHPTKREIRFSHPEFIHTGVLRGIKALFFTQ
                     PSSDILSDENTEKAGPAPHSARPPSRSLTVAPAPFQHVSAFPGGGTNRHPTLSLFVQE
                     PPAVYAGREEPREVYPLGQIHHTYLLGQIDQDLFIVDQHTAHERVRFERLLRSWKKKE
                     ILQQPLLIPEPVDLLPHQGELLEEWRPLLAQAGLEVERFGTTSYVVRAIPAILGNISV
                     GPLVLELLDELSEWQSTDSLDNTIKPILATMACQSAVQAGRPMTQPEITILLQDWAQE
                     NFPMTCPHGRRVAVRHSLEELHTIFARPLK"
     gene            9920..10945
                     /gene="miaA"
                     /locus_tag="VLA60_06155"
     CDS             9920..10945
                     /gene="miaA"
                     /locus_tag="VLA60_06155"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HSF08978.1"
                     /translation="MKQADIISAWKPVVAIVGPTAIGKSRIGIEVAKILNTEILTADS
                     RQVYRGMDIGTDKPSLAEQQNIPHRLIDLVNPDQSFNAGDFKRHAIQDIARLHRLGLL
                     PLVIGGTGLYIRALLRGLCPGPPADWQIRSELAQEAKIQGPAFLYEKLQQVDPELAQR
                     LHPNDQPKVQRALEVYRILGTPLSVIQQQHRFDEAPYPYLLIGLTMERQTLYQRIESR
                     VDWEIHKGLVEETQSLMNQGFLRELGSMKGLGYRQFSGFLAGDYSYEEAVRLLKRDTR
                     HFAKRQMTWFQKESEIQWMTLEESDIPDRAAAKIVDQINQFLSTFVNLPLPAEATSHL
                     KSTQSSG"
     gene            11081..>11521
                     /locus_tag="VLA60_06160"
     CDS             11081..>11521
                     /locus_tag="VLA60_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HSF08979.1"
                     /translation="MRESEEAKTKRASLRTQQAQIGIIGGSGLYEMEGFTNLKEVRMA
                     TPFGKPSDAIILGTLHGMRVAFLARHGRGHRTLPSAINYRANIYALKALGVTRIFSIS
                     AVGSMKESIRPGDFVLPDQFIDRTTQRANTFFDQGIVAHVAFADP"
BASE COUNT         3243 a         3138 c         2480 g         2660 t
ORIGIN      
        1 tcgttcagct aggtttagga cgccagattg tcaatctgga ggtcgcgggt tcaaatcccg
       61 tcggccccgc catacctcat ctcccagtgg tttatttctg tatcacttat cccctgttta
      121 gattctgacc agcctagagg aggtcgaaac acaatctatg acctttgctg ctaatccttc
      181 tgagttcttt ggatctttgg gcctgctttc cattgtcatt ctttttatcc tgtcaatttt
      241 ttccgtgatt tcatggggaa tcatcgtgaa cagattcagg cggtttagtc gcattcgaca
      301 agaagaatca caattcctac aattgtatga gcaaaatccc atcgaccagc acactttaag
      361 aagccaggct caaacattag cttatagccc aagttcagtg gtgtatttgg gcattaccga
      421 ccgcttgcca gaatcctccg atctcttcac ctcgagaccc cccatggagg caaaacacac
      481 agagcgctcc cctaatcgac agtatttgga aaaggtcgct caatacatca tccagaacca
      541 aatcggccaa caggaagcct atttgccttt tttggcgacc acaggaaatc tgactccatt
      601 tattggtctc ctcggaaccg tcttgggcat tatcaatgcc ttccatgaaa tcggccttca
      661 aggttcagca agtattgccg cagtcgctcc tggcgtctct gaagcacttg tcgcaactgc
      721 tgctgggcta tttgccgcaa ttcctgctgt gatggcctac aactattttc tctctagaat
      781 tcgaaggatg tccttcagag ttgaggcgtt tacaattgaa tttctcaata cgatagaaga
      841 agcggaaaaa gcctttgagg tggaagtaac ccgatgaaca ctggatcaaa gcctcggcaa
      901 tttctctctg aaatcaatgt cattcctctc gtcgatgtcg tcttagttct gctggtgatt
      961 ttcatggtta ccgccccgat gctacatcgg ggcttagaca ttaacctccc gacgacaact
     1021 gccaacaata taaaagccga agaacgattg attgtgacaa ttcagcggga tcacacttta
     1081 tccctcggta acgacacgat tagtctggtc aatttacgag ccaaacttca agaagccaaa
     1141 accctaaacc ccttgatttc agtctatctg cgagcggacc agacggttcc ctacggaacc
     1201 gtcgtgcagg tcatggatga ggtcaaaggg gccaaaattg aacgtctcgg aatgattacc
     1261 ggcccgaaaa ttgaaacact aatcgaggac gagactatcg ggatttcccg ctaagcgttt
     1321 gaccaatggg acatgctgaa tccccaagtc taatttccct ggggctcgct actgacgttt
     1381 ctcaaaaaga cgcctcattc cgttggggac tcgccacctc aggtcttttc catgctatcg
     1441 tcatcatgat ggccctgttc ctacgatttc aatcgacggt tgaggaacct ctccgggcca
     1501 tcgatgtcac tcttatttct gtacccgaaa cccaagctcc ggctccaagc caaaaaaaat
     1561 cttctccccc accgccgcca tcccaacaaa aatcccaacc aacaaaaaaa atagagccaa
     1621 caaagacgca accaatagcc cccccccttc ctcctttgcc ggtcccaaaa gcctccgagc
     1681 gattatcgga attcatggaa ggggctgttg gctcaatcgt gatgcctcac aaacaagaaa
     1741 tcacttcacc aacacccccg caacccataa acgaacaacc tccctcgaat gaccagtcac
     1801 cgttgtttga aaaccttcga atgcccccta ttgctccaca aatttcacgc ccggaacgct
     1861 tacattcatc acaaccctta gtcattccca agcctactcc aactccagcg ccatctcctc
     1921 aagaggctgc gcccccaaca acggttcctc ccgagcctca agggtcggaa tcacaacctc
     1981 aggctcctaa aattgaacca actgtcaagc tagctccggt gctaccggaa ttgagttcgg
     2041 tgactccatt cagggcatcc aaaaaagaaa gaaaacccaa cgacgcttct ccgttaaaca
     2101 atcttgagga atctctcaaa cgaaccatcc caacagttcc gatacctgcg ccaactccaa
     2161 aaatcaaaaa acagcacaaa accattacac caaaacagga aaaacctttc gtccctgaaa
     2221 tctcccctcc tcaattagcc caaatctctc cctccccacc actggaaaaa gctccacaac
     2281 gtgagaaaat gtcagatatg atgaagcaat tactggaaga agctagagtc ccaaatttac
     2341 aatcgtctcc acccgcacca tcctctcaac aaccattgcc ctcttcttcc acatcaaccc
     2401 agccggtcca gtcggaaatt gatctacaaa ttgcaaaatt atctattccc aatgtcacac
     2461 ccgtcgaatc gatcaaggaa cgactccaac ggctcgaggt gcagcccaac tcaaaaacag
     2521 gccaatcgtc gtctccgtct tctcctggca aaaaccaata tttggcaatg gtcgaggaca
     2581 gaattgacca cgagtggaga gcccttccac tggtcacaaa ccctccggtc gtggtcctca
     2641 aattccgcat ttcccgattt ggagaaattt ctaatattca cattgatgaa agttcaggaa
     2701 acgggaacta tgactccgca gccaaacgag cggtatctgc cgtgaaccca ttaccacctt
     2761 ttccaccaga catttccgat tcattcttcg aagtacgatt tcgattcatc aaaaaagatt
     2821 agaaggacac cagctatatt atgaagaaag cactcaaccc tttgtatgcc attctggctc
     2881 ttctcgcctt ttcggttatt tcggtatcac cggcctttct tcatggagag gaagcggatc
     2941 ttaaagccac acgttcagag tttcaaaaaa tcccgatttg ggtaatgggg ttctcgcccg
     3001 tccaacgaga taccggcatt tcagaagacc tcgagtctca agtcgcatcg gtcctaaaag
     3061 cggatctcaa acgctcacaa atattttcaa ttgctgaatt acctcctgcc aaaggggatt
     3121 tttctgacaa taaatgcatg agtctttctt caaatttggc tccgcacttc caaaaggtga
     3181 cagtctctac ctgggggaga ataggtgtgg gagggacatc gagcggagtt cagggactta
     3241 tccttgatgc ctgcgcattt gatcccggac aacaggatgt gttgacaggg aaacggtact
     3301 tttcccttaa aacgacagaa accctcactc gcttaatggc tcaccggtgg gcagacgaac
     3361 tggtttatcg gtataccggc gagcagggca ttgccagaac caaaatcgca tttgtgggtc
     3421 aaaaggacaa tggacgagag cttttcgtca tggattacga tgggtataat ccgcaacagg
     3481 ttaccgctga cgggtattta aatctcatgc ctacctgggc cccggaccgg aaatcactta
     3541 tttacaccgc gtatcgtgac cggaaacaac tgattataaa aagggagctg gctaccggtc
     3601 gggaggaagt tctggtctcg ccagccagtc tgaacattac cgccaccttt gctcctaatg
     3661 gaaaatctat cgcctactct gcggcccaag atggcaactc ggatatttat cagatagaat
     3721 tggacagtag aagcgtgaag cagttaacct ctcataatag cgcagatttg tccccgtcat
     3781 ggtctcccga cgggcggcat ctggccttca cctcagatcg gggaggccgc ccccaaattt
     3841 acatcatgga tgccgatggc ttgaacgccc gtcgattaac ctatgatgga gactataacg
     3901 cggcgccggc ttggtctcca aaaggagatt ggatcgtcta tgtgtgtcgg ataccaaacg
     3961 agggatttaa attatgccgc atcagtccga atggggaaca gcggaaacaa atcaccaacg
     4021 ggcaaagtat tgatgactcg ccttcctggg ctccaaatgg acgccatatt gtttttagtt
     4081 cgattcgaag aggagaaagt caattataca tcatcactag cgagggggca ggattggaga
     4141 aaatttcctt ggcaggcgaa catctcagct ctccctcctg gtcaccgttt caaccataat
     4201 taacgagaca ccaaccgtga tagtccaaaa aaggagtcgt tccatgcgcc cagccattca
     4261 tatgatttca cttcttggca ttctcagtct ttcactcatc agtatttcgg gatgtggaga
     4321 aaaacggatc cacgtctcca ccgcttccgg ctccccaggc gaagaaaccg gggaatcagc
     4381 atcaggcacc gatacggcag ggttaacagg gacctccatt aatgaaaccc ccctgtcagg
     4441 gcaaggcgct tccaccgatg gaattcgggc agaggcggtt caaccacccg ctccggttga
     4501 accttccaat tttggttacg accccaacca catggatgaa tcgttcaagc agaatctcca
     4561 agctggaatt ccttctggga atgactccga aattgagaaa aacgatgcca gcccatcgac
     4621 gccgttcaag gatggaaagg acatcttaac tcaatccagt tcttcaagta tgaacggctc
     4681 acctcacgat caaacagaga catccccctt ttcctcccag acggaattcc aaaatgatct
     4741 ttcgaatttg ccaaaggaca tggaaccaat tcctgaaacg tttcaagtag cgaaagctga
     4801 accgtcagaa atcattcgag agcagttgga taaaattcaa gaggaagaat tggccacagt
     4861 atccgccggc ctagaggatg tattctttca ttttgatagt tggtctctca cggcagaagc
     4921 caaggattcc ctcgaacgag acatggcatg gctcaccaac gatccctcct cactgttaat
     4981 tattgaagga catgccgacc agcgtggaac gcaggcctat aacatggtgc tcgggaaaaa
     5041 acgagccatg gctgttcggg atttcctttc tcaactgggg gtcgatcctt cgcgattgac
     5101 cgtcatttct tatggcaaag acaagccctt ttgccaggac actacagaag tgtgctacca
     5161 attgaaccga cggggacatt tgcttgttca aaattagtaa ctcctttcaa tcgaccagtt
     5221 ttcgaaaagc atggagggat aaatccagag tgaacctagg gcggaccttg caacatgttt
     5281 caagattgtg gacatcgact ttttctctca actggatagt ggtctatggc gtctgtagtt
     5341 tattgggtac gggttgcatg gctcaacaag ctgatcttgc ccgaatccaa aaggacttag
     5401 aacaacaaat cgccaagatc aaagaagaaa aaaagaccct tggcgcacaa gttgacgaaa
     5461 ccaaggcgca actcatcaag atgaatgagg aagcccaaaa aacacggggc aatctggctt
     5521 ctattaatca aaaagccaca ctcctccaag aaaaggatgt ggcgagttta tacggaaaac
     5581 ttgaagaaac tgaaaaaagt atccaggacc tcaaaaagga tttcacgaac caaactggcg
     5641 ctttgaaatc tgacgtccaa tcgattcagt caacaattca aacacatgga gaagacctcc
     5701 aaacagtaaa aacccaaaac acaaacctcg cccaacaggt ggatgaaaac aatttagcgt
     5761 taacctcaaa catgggcgaa tttcagaatt cccttaccca attcaaggaa acgctgacca
     5821 gcctcggagc gacgattgga caggtccaaa cagatttgtc aggacaacgg caagacctgg
     5881 gaaccgtgca atcccgcatg gaagaactac ctagggtgtt caataccagg cttgaaaagc
     5941 aatcaaaaca aatcactcag ctggaaaatc aggtcaccac cctgcaagag aaacttaccg
     6001 ccgacaccca agcccttcgt tcctatctgg aacaagacgt gaaagcatcc atggcgaggt
     6061 tagttgccga tatagacgct caccaaggtc cagtgctagc gcagattgat tccttacaaa
     6121 aagatatgga agctctggga acgcacgtgc aagcggatgc ctctcacgtg caggatctat
     6181 cccaatcagt tctcaagctt cgcgaagccc aggaagtcat gggaagttta ctcgggaaac
     6241 gcggagatga aattattcaa caagcgggac gaatttcgga acgcatgaac atgattgaat
     6301 cacaccaaac cgccctgacc gaacaactgc aatccaatac ccaaaaaacg tcagcccatc
     6361 tagccgaggt aaatgccagc ctcacctcca ttactcaagc acttgaccaa gccaggcaat
     6421 cactctccag tcgcctggcc aaacacgaag aggcaggaca aacactcaat cagtcggtcc
     6481 aacaaattca acagttgaag cgagacgtcc aggaccaaaa gcaacaagcg caggcggtca
     6541 accagattgc cgatcaactc aaccagacgg tcgagcgcat tggctcacga ttaaaagacc
     6601 ttgaaactca tcaatctggg ctggtgggga aaattgactc tgacacccag atgactaata
     6661 cccatctcca agaagtcaac agcgggattc aatcggtcgc ccaagcattg gaaaatgtca
     6721 gtacgaagtt gaatgctcgc attgaaagcc aggaacagcg gctgaatcga gccatgacct
     6781 ctttccaaca ggtccagggt accgctgata cctcacaaaa taatctgact cacctcaatc
     6841 agttaacaga aactgtcaat aagttgcgcg atgtgattaa taccattggg accaaattag
     6901 gcgagcgagt ggaccagcat gaagaccgtc taggccaatt ggcccaaaga gtcaatcgct
     6961 tacaaagccc caaaccgcaa aaatagggta cctcatcgct taatattctc aaagaaaggc
     7021 cgactatacc tctatactgt caaaaacccc tttgtctaaa ctccctggca attgataaga
     7081 tatgaggcaa agggtaactg actccctaat ttcaaacatt atttgttgtc gaagcctgaa
     7141 tttcatgact cccaaatttt ccactttgtc catctgtatt gggtttgccc tcacgggagg
     7201 atggggttgc gcaaccgaac ccaatttgac tgacatccaa aaacaagtcc aaaccctcat
     7261 cattcaacag gaagcgtccc acaagcagga acaacaaatt ctcgaccgca tggagacggt
     7321 tgaatcgcaa ttggacgaac atgatttctt agtgggagaa ctcatcaaaa cagaagagga
     7381 agccagtctg gatactcgac accttctgga gaagcttgaa cggaccagta ctctgctccg
     7441 agaacaaatc gaacaaaccc gctccaccac ccaacgccgt gatcaggacc tgtccattcg
     7501 ggtaaaggct ctggaaagcc gaatagacaa tgtgatccat caacctcgat ctgtgtcaga
     7561 atctcctgaa gtcacgactc ctaacaccac cagcccttct tccgatccga aagcgaccca
     7621 gacaggaatc cccgcatcac cgacctcccc tggagaacgc acgggagcgg aaaacgaggc
     7681 atcggccttt cggtcagcct ataaagttta ccttaacgga aattatgacc gggcctcagt
     7741 ggaattccaa cgatttgtca caaattatcc ttcgaccacc ttaactcctc aggcctatta
     7801 ctatcttggg gaatcgttct acgtgcaaaa acaatatgac cctgccaagc aagctctaga
     7861 acaagtcatc agccgctatc caagcagcaa atatcggagt cacgcattat acaagctcgg
     7921 acagattatg cttgaaatcg atcaacgatc aaaagcgcag gaactctgga actccatcat
     7981 ccaagattac ccagattcac cagaatccac ccaagcgaag gaacaattaa aaaaatctgg
     8041 gctgtcttga ttctctgcaa gtcttcgcat tctattgttc accctaatca ccccgtgctt
     8101 ggaatcccat gctttcgacc gtaactggaa aagttcaaat tcttccagac agcgtgtcat
     8161 gccgtattgc cgccggagag gtggttgaac gaccagcctc cgtagtgaag gaactgatcg
     8221 ataacagcct ggatgctggc agcacgttga tcaccgtgga ggtcgaagaa ggtggtcgac
     8281 gcgtcattcg cgtcattgat aatgggacag gcatgccgcg gatggatgcc caactggcct
     8341 gtcaacgttt tgcaaccagc aaacttcgat ccgaagacga tctcttgaca ctcatgacga
     8401 tgggattccg aggggaagct cttcccagca ttgcctccat tgcccgtttt tgcttgaaga
     8461 cagttccacg cgatagcacg attggcaccc aaacacagtc cacaggaggc gtggcatggg
     8521 aagttcaaga ctataccggt cctcaaggca ctcaagtgga ggtgcgtgat ctctttttta
     8581 atactccagg gcggctgaaa tttctcaaga ccgttcccac ggaattttct aaaatttgtt
     8641 atgtcgtcca acaggcggcc gccgtgcatc cagagatcca ctttcgatta cggcaccaga
     8701 accataaggt gctggagtac cccgccgtgt cctcgctgca agatcgacta ttacagatct
     8761 acggaccaga cttccttgaa cgctttctcc ccgtcaccta taacgctggt tcatttcaac
     8821 ttacagggta tacgatcagt cctcaccatg cacgagcgtc tcgtgcgcca caggaaattt
     8881 ttgtcaatgg acgccctata aagaatacga ccatatccca tgcggtgctg gaagcctacg
     8941 gatccttttt accaaaaggg aaacatccac aatttattgt atttatccat cttgatcccc
     9001 agtcaatcga tatcaatgtc cacccgacga aacgggagat tcgcttctcc catccagaat
     9061 ttattcatac gggcgttctc cgtggcatca aagctctctt tttcacgcaa ccctcatccg
     9121 atatcttatc cgatgaaaac acggagaagg ctggcccagc tcctcattct gccaggccac
     9181 catccaggtc actcaccgtc gcaccggctc cattccagca tgtttctgca ttcccaggag
     9241 gcggcaccaa tcgacatccc actctttctc tatttgtcca ggaaccgcct gctgtctatg
     9301 cgggcaggga ggagccaagg gaggtatatc ctcttggaca gattcatcac acatatcttc
     9361 ttggacaaat tgatcaagac ctattcattg tcgatcaaca cacagcccat gaacgggttc
     9421 gttttgagag actgcttcgg tcatggaaga aaaaagaaat tcttcaacaa cccttactca
     9481 ttcctgagcc ggttgacctt ctgccccacc agggagaatt attagaagaa tggcgccccc
     9541 tcctggctca agcgggattg gaggtggaac gatttggtac cacatcgtat gttgtacgag
     9601 ccattccagc catattagga aatatatccg ttggtccctt ggtcctggaa ttattggatg
     9661 aactctcaga atggcaatca acagattcgc tggacaacac gattaaaccc attctggcta
     9721 ccatggcctg tcaaagtgcc gttcaagccg gacgacccat gacgcaaccc gaaatcacca
     9781 tccttttaca ggactgggca caggaaaatt ttcccatgac ttgtcctcat ggaaggcgag
     9841 tggctgttcg acattccttg gaagaattgc acaccatatt tgcccgtcca ttgaaataat
     9901 tttcattcgt tgatggttca tgaaacaggc cgacatcatc tcagcatgga aacccgtcgt
     9961 cgccattgtg ggacccaccg caatcggaaa aagccgtatc gggattgaag tcgctaaaat
    10021 tcttaacacg gaaattttaa cggcagactc ccgacaggtc tatcgtggga tggacatcgg
    10081 caccgacaaa ccctcattag ccgagcaaca aaatatcccc catcgcttaa tcgacctcgt
    10141 caatcccgac caatccttta acgcaggtga tttcaagcgt cacgctatcc aagacattgc
    10201 ccgcctgcat cgcctgggtc tcctacccct tgtcatcgga ggtaccgggc tctatatccg
    10261 agccctctta cgaggtcttt gccctggtcc accggctgat tggcagattc ggagtgaatt
    10321 agcccaggaa gccaaaattc aaggacccgc ttttctttat gaaaagcttc aacaagtgga
    10381 tcccgaactc gcacaacggc tacatcccaa tgaccagccc aaggtccagc gcgcacttga
    10441 agtgtatcgc attcttggca cgcctctatc ggtcatccag caacaacacc gttttgacga
    10501 ggcgccgtat ccctatttat tgattgggct caccatggaa cgccagaccc tctatcagcg
    10561 aatagaaagc cgcgtcgatt gggaaattca caaaggtctt gtggaagaaa cgcaatccct
    10621 catgaatcaa ggatttcttc gagaactcgg ttccatgaaa gggctgggct atcgacagtt
    10681 ttcaggattt ttagcggggg actattctta tgaagaggct gtgcgattat taaaacgcga
    10741 tacccgtcac ttcgccaaac gacaaatgac ctggtttcag aaagaatctg agatccagtg
    10801 gatgacattg gaagagtcag acatccctga tcgagctgcc gccaaaatcg tggaccagat
    10861 caatcagttt ctctctacct tcgtaaattt accacttcct gcggaagcaa cgtcccattt
    10921 aaaatccact caatcgtcgg gttaacccta caggaccgtt catgatacaa tgccctcgct
    10981 cgttccgcag agacatgaaa gactacccat tagttggggt caaatcagca tacaaccaaa
    11041 accacatgtc tttccccttt aatcagagag accctttcac atgagggaga gcgaggaagc
    11101 gaagactaaa cgggcctctt tacgtaccca acaggcccaa atcgggatta tcggaggcag
    11161 tggactctat gaaatggagg ggttcaccaa cctcaaagaa gtccggatgg ccaccccgtt
    11221 tgggaagcct tcggatgcca ttattttggg aaccttgcat ggcatgcgag tcgccttttt
    11281 agctcgccac ggtcgaggac accgcacgct accctccgcc ataaattatc gcgcgaatat
    11341 ttatgccctc aaagccctgg gtgtgacccg gatattttcc atcagtgcgg tgggaagcat
    11401 gaaggaatcc attcgaccgg gggattttgt cctgcctgac caattcattg accgaaccac
    11461 tcagcgtgcc aacacgtttt tcgaccaggg gattgtcgca catgtggctt ttgccgatcc
    11521 c
//
LOCUS       DATIAL010000195         2014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_465649, whole genome shotgun sequence.
ACCESSION   DATIAL010000195 DATIAL010000000
VERSION     DATIAL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465649"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            568..>2014
                     /locus_tag="VLA60_06165"
     CDS             568..>2014
                     /locus_tag="VLA60_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSF08980.1"
                     /translation="MSIPEDQACSKEGRERALIDYPPIEQHGVIGDLHTVALVGMNGS
                     IDFMCFPRFDSPTIFASLLDQRHGGEFSILAVRDTGQRKQLYLTDTNILLTQLYAEEG
                     MGEISDFMPIEEAGQIHNVIRRVKTVRGRMQYCMRCAPRFNYASATHSIEMHHDKEVV
                     FISHGPDQTRLRLRASIPIQVNDQDVIAQFTLQAGEHATFILEDAGNSEQSAASNPED
                     VSDVFKATMNFWHNWLASCRYEGRWRETIHRSALMLKLLTFRPNGAIVASPTFGLPEI
                     MGGERNWDYRYTWIRDAAFTVYAFLRLGFTQEAVAFNEWVEDRFSHMNGQHPLQVMYR
                     IDGSTDLLEQTLPNLSGYKGSSPVRIGNAAHEQLQLDGLGALMDSVYLSNKYGRLVSY
                     DFWKSLAHLIDWVCDNWQLPDHGVWEFRGQPREFLYSRLFCWVAVDRGIRLAKKRSLP
                     GPIRRWKQVRDEIYQEVMQDFWNPELKCFVQY"
BASE COUNT          482 a          466 c          502 g          564 t
ORIGIN      
        1 cctccaccac ttgagaaagt tccttgataa gttttcgatt ttgttcctga agatataaga
       61 tttcctgctc taattgctcg atatttaaga aatgggtcat caatccctcc ttccctcctt
      121 gttaaaatcc agccgtcttc gctgcttggc cgatccaata ttaaacattc tccctgtttt
      181 aaacattctc cctgttcgga ttctttctgt atcttatcag gcgaatcaac gcatggtgaa
      241 atcgtttgtt agatacagga attttcgctt acatgatatt gattttgagg acacctctga
      301 ctcgtgagcc atgaaccagg gtttctgcca gtaggggaga aaatatttcc tggtagcata
      361 cctttcaaga actgaccaga tcgttaatgt atgcaggcga accttcttga aaaggaatgg
      421 tttctaaggt gggagttatg tcggtcactg gtcaatgacc cctcactcac agcaggtgga
      481 cgaagggaag aaccttcacg gccttctcgc catcgggctc aagctcttta tttttggtct
      541 atccatttgt gacaggcgag tacaacgttg agcattcctg aggatcaggc ttgttccaaa
      601 gaagggagag aacgagcgtt gatagattac ccgcctatcg aacaacatgg ggtcatcggg
      661 gatctgcata cggtggcgtt agtggggatg aacggttcga tcgatttcat gtgttttcca
      721 cgttttgatt cccccaccat ttttgcctcc ttgctggatc aacggcatgg tggagaattt
      781 tctatccttg ccgttcgtga taccggtcaa cgaaagcaac tttacttaac cgacaccaac
      841 attctgttga cgcaattgta tgccgaagaa gggatgggcg aaatttccga ctttatgcct
      901 attgaagagg ccgggcagat tcataatgtg attcgtcggg tgaaaaccgt gcggggacga
      961 atgcaatact gcatgcggtg cgccccgagg ttcaattatg ccagcgcgac tcattccatt
     1021 gaaatgcatc atgacaaaga agtggttttt atttcccatg gtcccgatca gactcgctta
     1081 cgacttcgcg cgtccattcc tatacaagtg aacgatcagg atgtcattgc gcaattcacg
     1141 ctccaagccg gggagcatgc caccttcatt ctggaggatg ccggcaatag tgagcagtct
     1201 gccgcgtcca atccggagga tgtatcggac gtctttaaag ccacgatgaa tttttggcat
     1261 aactggttgg cctcctgccg gtatgaaggt cgctggcgag aaaccatcca tcgttccgct
     1321 ttgatgctga aactgttgac ctttcgcccg aacggagcga ttgtggcttc ccccacattt
     1381 gggcttccgg aaatcatggg tggagaaaga aattgggatt atcggtacac ctggattcgg
     1441 gatgccgctt ttaccgtata tgcgtttttg cgcctggggt ttacccaaga ggccgttgct
     1501 tttaacgaat gggtggaaga tcgctttagc catatgaacg gacagcatcc tctccaggtc
     1561 atgtatcgga tcgatggtag tacggatcta ctcgaacaga ccctaccaaa cttgagcgga
     1621 tataaagggt cctcaccggt ccggatcggc aatgccgccc atgaacagct tcaactcgat
     1681 ggattggggg cactcatgga ttccgtctac ttatccaata aatatgggag acttgtctcc
     1741 tatgattttt ggaaaagtct tgcccatctg atcgattggg tctgtgataa ctggcagctt
     1801 ccggaccatg gtgtatggga atttcgcggg caacctcgtg aatttttata ttcccgcttg
     1861 ttctgttggg tggccgtgga tcgtggaata cggttggcaa aaaaacggtc cttacctgga
     1921 ccgatcagac ggtggaagca ggttcgggat gaaatttacc aagaagtgat gcaagacttc
     1981 tggaatcccg agttgaaatg cttcgttcaa tata
//
LOCUS       DATIAL010000196         5224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_788294, whole genome shotgun sequence.
ACCESSION   DATIAL010000196 DATIAL010000000
VERSION     DATIAL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788294"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VLA60_06170"
     CDS             complement(<1..626)
                     /locus_tag="VLA60_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF08981.1"
                     /translation="MKDGSLIPQKTGVSSSRLTIEKGNGQRISAYHDQPIGGNLTEAP
                     VVVIAPGYGETKRDYLTLAYYFASNGFHVVRYDHTNHVGESDGRHYQISLSSMKNDFQ
                     TVTRYVRVTWPHSALIGVAASLASRVALKAEAECPSLSLLIMLMSIVNVQRSAATVHQ
                     EDLFAAYGNEQCPASANFLGFNVGNQFLGDAIANNFVTIEDTLIDAES"
     gene            1637..3739
                     /gene="fusA"
                     /locus_tag="VLA60_06175"
     CDS             1637..3739
                     /gene="fusA"
                     /locus_tag="VLA60_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HSF08982.1"
                     /translation="MPHESAPSIRNVVICSYPGAGKTTLCEALAFSTGVIPTMGTIPQ
                     GNTIGDFEPEEVHRHHSISSTICQFEWQGTKVNLIDTPGMSDYAFEVQSALRAVDGVV
                     LVVGASTGLRSEIERVWDLIQEHELPCLLFINELDKERADYRSILTECEKTLGFTGVP
                     ITIPVGGEATLTGVIDLISRNVITPASGTPKIHQTEISPEHQPIANEFRRRAMEQVAE
                     TNDQLLEKYLSQGEISDEDLQDGLTLGTLERKLIPVLCGSAIGNIGTTTLFDAIQRYL
                     PSPSDRSSLHPNIGLQPLTHDEGQRKSDPHDPFSAFVFKTTIDPFMGRLSFVRVLSGT
                     LHADSGFYNASRQTKEKGGHLFFSVGKKHHQVNQVSAGDIVAIGKLKDTQTGDTICDE
                     RHSIIFPGLKIARPVMSYALEVKSKNEIDKVSLGLHKLVEEDPSLEFLRNEETKEMLL
                     SGVGQSHIDATLDKLRRKYGVEVDLHMPKVPYKETIHRMAQAQGKYKKQTGGHGQYGD
                     CWLQIEPLPRGAGFEFHNKIVGGVIPRNFIPAVEKGVVEALQHGIVAGFPIVDIRVAV
                     YDGSHHPVDSSELAFKVAGSMALKHALEAAQSTILEPIMSVEVLVPDDLVGTVIGDLN
                     SRRGRIQGMASKGHNQIVKAFVPLAEMLKYAPALTSMTAGKGSYVMEFSGYEEVPKDC
                     LNRIVEEHKKDKEAVSSH"
     gene            complement(3764..5089)
                     /locus_tag="VLA60_06180"
     CDS             complement(3764..5089)
                     /locus_tag="VLA60_06180"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HSF08983.1"
                     /translation="MWISPSPAVSQTANELRGLQLLGDIEQAITSLAERVTPTVVNIT
                     PIREIAQGQGFQRRAPYSQGSGSGVIVREDGIIVTNNHVVGEDAREADVRLSDKSNMI
                     ARVIGRDKETDIAVLKIESDRKFPVAHFGDSNALKVGQWVLAVGNPMGLDRTVTLGVI
                     SGIGRERLNLSRYENFIQTDAAINPGNSGGPLFNLRGEVIGINTAIIHMAQGIGFSIP
                     ADMVSRVVDQLVSQGRVVRGWLGVGIQSLTKELAEQFGIKEGHGVLVNEVYEHDPAHV
                     AGIKPGDIIVSVDDSSVDSPNQLSRLVARVGPGENAKILVLRDGEELTFLVPMAERQD
                     KSMLTSLPLQKSEVTLGLDVQGLTAALAEQFELEETVGVLVTKVEPGGLANSEGIQEG
                     DLINEVNRQTVRTVTQFSEEVAKVKPGQTILLRIIRKARAFFIVIKTLP"
BASE COUNT         1377 a         1275 c         1231 g         1341 t
ORIGIN      
        1 aaggattccg catcaatgag tgtgtcctca atcgtgacaa agttattggc aatggcatca
       61 cccaaaaatt gattacccac attgaagccc aaaaaattcg ccgaggcagg gcactgctca
      121 ttcccatagg ctgcgaagag atcttcctga tgaaccgtgg ctgcagaccg ttggacattg
      181 acaatgctca taagcatgat caagagggat aaggatggac attcagcctc ggctttgaga
      241 gcgactcgcg aggctaagct ggcagccaca ccgattaggg ctgagtgcgg ccaagtgacc
      301 cttacatacc gggtcacggt ttgaaaatca ttcttcattg atgaaaggga tatttgatag
      361 tgccgtccat cgctctcgcc gacatgattg gtatggtcat agcgcacgac atgaaagcca
      421 ttgctggcaa agtaatacgc caaagtcaaa taatcccgct tggtttcccc ataccccggg
      481 gcaataacga cgacaggtgc ttctgttaaa tttcccccaa taggttggtc atggtaggca
      541 ctaatccgct gcccattgcc tttttcaatg gtgaggcgac tgctggacac gccggttttt
      601 tgaggaataa gggacccatc cttcatccct tcttccttct caggaaaaga aacacctgat
      661 attgagtcga agagttcgtg tttttgaatt ttccagggcg aattgaacga accatttaaa
      721 acctgtccat ggaccatgag gctttctcct tgaggcagca ggactttcgt tcctgacgaa
      781 aatgaaccag tggcatcatt ctctgttggg cagtctagaa gaatgtctgt tgtccctttc
      841 cacgccgtct gacctggttc cccctgtata tctttaatca ttccatggag cttttttagg
      901 ggaagatctt gacctgaaat acaatatttc aactcaatga tctgcgagag ctctatggga
      961 ggaaatccgg tcaaacgcaa gtgcacagtg tcctcgtgcc atccaataag gtacccctcc
     1021 caagtccctc caagacaatg caaagagaag ggaagtggcg agaggtcacg ggctctagaa
     1081 agatcaccag gaactagctc ctgagttatc tgcttcaatc cagcacattg atacattgaa
     1141 gttctacccg ctaactctgg tgttaatgtg ctcatgcgat cccccaccta aatataagtg
     1201 ttaataccta gtttttgacg caagactttc cctggatctc aaaagatcct gatgtctgca
     1261 tcagcctgta cgagaagagg ggcgagagat gaaacgactt acatcggttc gttcattgat
     1321 acgggtgtat gggccaccat ggaattggta tcaattttac tctctcttgg ttaggtcact
     1381 agagcaacct gagtgccaga attttcaaac aaaatgctcg aatctatttt caagagaaaa
     1441 acacagaatt tactgggaaa tttttgcctg aatatttttt tcccggaatt gcagtgcaga
     1501 atttttaaag tcttctctgt cggtccactg acgataaatt aaacagagcg ttggtctcct
     1561 gacagcccat tgttcttttt cattgacact ccttcctctc ctacctaaaa ttcaaggaaa
     1621 ttgagaggag gttcgcatgc cacacgaatc ggccccatcc atccgtaacg tggttatttg
     1681 ttcctacccg ggggcaggaa agaccaccct gtgtgaagca ctggcctttt ccacgggagt
     1741 catcccgacc atggggacca ttcctcaggg aaataccatt ggtgattttg aacctgaaga
     1801 ggtccacagg caccattcca tcagttcgac catctgccag ttcgaatggc aaggcaccaa
     1861 agtcaatctt atagatacac cggggatgag cgattatgcc tttgaggtcc aatccgctct
     1921 cagggcggtg gatggcgtgg tgctggttgt aggtgccagt acagggctac ggtcagaaat
     1981 cgaacgagtc tgggacctta ttcaggaaca cgaacttccc tgtcttctgt ttattaatga
     2041 actggataaa gaacgtgcgg actatcgctc aattctgaca gagtgtgaaa aaacattggg
     2101 attcaccgga gtccccatca ctattcccgt tggaggggaa gccaccctga ctggggtaat
     2161 tgatctcatt tcgaggaatg tcataactcc ggcttccggc acgcccaaaa tccaccaaac
     2221 ggagatttct ccagaacacc aacccattgc gaatgaattc cggcgccgag ctatggaaca
     2281 ggtggcagaa acgaatgatc aattgttgga aaaatatctt tcccaaggtg agatctcaga
     2341 tgaagattta caggatggat tgacgctcgg tacattagag cggaagctta ttccggtatt
     2401 gtgcggatct gctattggca atattggaac cacaaccctg ttcgatgcga tacaacgata
     2461 tctcccgtct ccttccgatc gttccagtct ccatcccaat atcggcctgc aacctctgac
     2521 gcatgacgaa ggtcaacgaa aatctgatcc acatgatccg ttttcagctt tcgtctttaa
     2581 aaccaccatc gacccattta tggggcgtct atcatttgtg cgagtgctgt ccgggacctt
     2641 acatgcagat tcaggatttt ataatgcctc ccggcagacc aaggagaagg gtggacatct
     2701 ctttttctcc gtggggaaaa agcatcacca ggttaatcag gtctccgccg gagatattgt
     2761 ggccatcggt aaattaaagg atactcaaac cggcgacacc atttgcgatg aacgacattc
     2821 cattatattc cccggcctga aaatagcgcg gccggtcatg tcgtatgcct tagaggtaaa
     2881 aagcaagaat gaaattgaca aggtgagttt aggtcttcat aagttagttg aagaagatcc
     2941 ttctctcgaa tttcttcgga atgaagagac aaaagaaatg ctgttaagtg gggtagggca
     3001 gagccacatc gatgccacct tagacaaact gaggcgaaag tatggcgttg aagtggactt
     3061 gcacatgccg aaagtccctt ataaggagac gatccatcgt atggcccagg cccaaggaaa
     3121 atacaaaaaa caaaccggtg ggcatggaca atatggagat tgttggctac aaattgaacc
     3181 tcttccccgg ggagccggat ttgaatttca caacaaaatt gttggtggcg tcataccacg
     3241 gaatttcatc cccgcggtgg aaaagggcgt ggtcgaagcc ctgcaacatg ggattgttgc
     3301 cgggtttccc atcgtggata ttcgcgtggc ggtctatgat ggatctcatc atcccgtcga
     3361 ttcttcggag ttggccttta aagtggccgg ctcaatggcc ctcaagcatg ccctagaggc
     3421 cgctcaaagc acgattcttg aacccatcat gtcagtagag gtgctagtcc cggatgattt
     3481 ggtgggaacc gtgattggag atcttaactc gcgccgtgga cgcatacagg gaatggcaag
     3541 taagggacac aatcaaattg tgaaggcctt tgtgccgctt gcggaaatgc tgaaatatgc
     3601 tccggctttg acgtccatga cggctggaaa gggtagttat gtgatggaat tttcaggtta
     3661 cgaggaggta ccgaaagatt gccttaatcg tattgtggaa gaacataaaa aagacaagga
     3721 ggctgtttcg tcccactgac cccctctcat attgtgcgtg gcttcaagga agtgttttga
     3781 tcacaatgaa aaaggcccgt gcctttcgaa taattcgaag taaaattgtc tgacccggtt
     3841 tcactttggc gacttcctcg gaaaactgtg tgacggtccg gaccgtttgc cgattgacct
     3901 cgttaatcaa atctccttct tgaatcccct cagagttcgc gagacctccc ggttccactt
     3961 tggtcacaag cacgcccacg gtttcttcta attcaaactg ctcagcgagt gcagctgtaa
     4021 gcccttggac atcaagccct aaggtgactt ctgatttttg taacgggagg gaggttaaca
     4081 tggatttatc ctgcctctcc gccatcggta cgaggaaagt caattcttcc ccgtcccgta
     4141 acacaagaat tttagcattc tctccaggtc cgacacgcgc cacaagtctc gacagttggt
     4201 tgggcgaatc aaccgagctg tcgtctaccg acacaatgat atcccctggt ttaatgccgg
     4261 ccacatgggc gggatcgtgt tcataaactt cgttgacgag aaccccatgg ccttccttaa
     4321 tcccaaattg ttccgcaagt tctttcgtga gagattgaat cccgacacca agccacccac
     4381 gaaccactcg cccttgggag acgagttgat cgaccacacg cgaaaccata tcggcgggaa
     4441 tggaaaatcc tatgccttgt gccatatgga ttatggcagt attgattccg atcacctcac
     4501 ctcgaaggtt aaagagaggc ccacctgaat tcccaggatt gattgcggca tccgtttgaa
     4561 tgaaattttc ataccgggat aaattgaggc gttcccttcc aatcccactg ataacgccta
     4621 gggtcacggt gcggtctaat cccatcggat ttcccacagc caatacccat tggccgactt
     4681 ttaaggcatt ggagtcccca aaatgagcaa ctggaaattt ccggtcagat tcaattttga
     4741 ggaccgctat atcagtttct ttatcacgtc caatgacccg agcaatcatg ttggacttat
     4801 cggatagccg aacatctgct tccctggcgt cttccccaac tacatggtta ttggtaacga
     4861 tgattccatc ctcccgcacg atgacacctg agccacttcc ttgagaatat ggcgcccgtc
     4921 gttgaaatcc ttgtccctga gcgatttcac gaatgggggt aatattgact accgtaggcg
     4981 tcacccgttc agccagactg gtgatagcct gttcgatatc tcccagaagc tgtagccctc
     5041 taagttcatt ggctgtctgg ctaactgcgg gtgacggaga aatccacaaa agaaggcaac
     5101 ataatgaaaa aatgagcgga aaattatcta aattcatcct aaaccttaca ttttacagtg
     5161 tcggataata aatattatgt taaatagaaa atagcctcat tttgctttcg gtcccctggc
     5221 taag
//
LOCUS       DATIAL010000197         4287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_680684, whole genome shotgun sequence.
ACCESSION   DATIAL010000197 DATIAL010000000
VERSION     DATIAL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680684"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VLA60_06185"
     CDS             complement(<1..353)
                     /locus_tag="VLA60_06185"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSF08984.1"
                     /translation="MGKSAQTEQDVQRPLSFPQRDRRTVRRVAVKFHQVLPVAIRCGQ
                     GMVCEGRVLNLSVQGMLVEFPKDQIPPVQMNAKVSVKLHYLGDSIWLPGLVRHRKGQK
                     MGFVFPALTNNPTRVG"
     gene            complement(356..721)
                     /locus_tag="VLA60_06190"
     CDS             complement(356..721)
                     /locus_tag="VLA60_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF08985.1"
                     /translation="MVVSERSLSDDTLVETFAGHFDQHARQDFKWRIDYAITQGFRFV
                     ILNMVAVSFIDSSALGWLVLAQRRFQRIGGRLGIIAPLGFVRDILELTEIDEWIPIFT
                     SEDEALKSLQFSREHPSES"
     gene            1189..2211
                     /locus_tag="VLA60_06195"
     CDS             1189..2211
                     /locus_tag="VLA60_06195"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF08986.1"
                     /translation="MMCFALPGWAQQQNGEHPAHPLASANTLLVEIVALSERGLENSS
                     AITKTITDKLSEAGFTVVSSPDQPHEAVIRVKCEERQTWTGPSKYRSISPVPTSRLWK
                     GPACHISYRYQDSSPQWKWEVRTSFEDIGAAAEAQGASNTGTFALNALNTELQQDDFP
                     LFILAEWEQTDRLLMLLQKSQGNSERQMTILQLLGTISSDKAFNALQNALSHPPLIPA
                     ALLALGQQGETAIPTLITFLEATLNSEHRLTAIQALGSIATYSKDPMLFTQFIQLLDS
                     EDPKVQTEAVKGLGNLGDRRAIQPLEKLNLQAWTNPSSSPDMRALREMLSSSLWQLRP
                     DAHTGG"
     gene            2373..4133
                     /locus_tag="VLA60_06200"
     CDS             2373..4133
                     /locus_tag="VLA60_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF08987.1"
                     /translation="MHRNKRRSQHPSIRKPGNSFLPPEVHRLFLQAVAHHQAKRFSQA
                     EDQYNRILTQFPSHADTLHLRGLLAYQQGQNSLALTYIKQAIAEDSGKPHYPYNLGLV
                     FEKEERWEEAIAAYQQALIVNPQYVEALANLGNVYRRQRQWIDAVTTLQQALQLKPQS
                     ADLHNALGVTYKEQGERENALRHYQQALQLSPHHAEALNNMGVVLQDQGQLDKAAEAF
                     QKALTVKPEYANAHYHLGLIRLWQGKESQALAGFHRSGDLTHNHGHGVAPLFVTKARV
                     KHDFEQLQYLTAQVSSRSISNDYRHALTNVYQRLSQSSPDSMFMKLSVQEQSQLAPSF
                     NRLLYIRPTSRLDGYAINPQLDVSAIETEYFSTTPEVMSVDGLLTEKALSEIRAFCLE
                     STIWKRDYPNGYIGTFLANGFACPLLLQIAEELRTRFPKIFQHYQLQQAWAFKHDSAL
                     RGLNMHADAAAVNVNFWITPNEANRNPESGGLVVWNKEAPDDWDFVEYNNDKNKHKIQ
                     TFLEESGAQPINIPHRQNRAVIFNSNLFHQTDIIEFQDIYECRRINITLLYGHRQQSR
                     QSGATRQTKKDERRVLKKND"
     gene            complement(4139..4214)
                     /locus_tag="VLA60_06205"
     tRNA            complement(4139..4214)
                     /locus_tag="VLA60_06205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4179..4181),aa:Arg,seq:acg)
BASE COUNT         1152 a         1131 c          976 g         1028 t
ORIGIN      
        1 cctacccggg tgggattatt ggtaagggca ggaaagacaa atcccatctt ctgccccttg
       61 cggtgccgga ccagacccgg caaccaaata ctgtcgccga ggtaatgcaa tttgacagag
      121 accttggcat tcatttgcac gggaggaatc tggtccttgg gaaattcgac gagcattccc
      181 tggacactca gattgagaac ccgaccctcg cacaccattc cctggccaca acgaatggcc
      241 accggaagca cctggtggaa tttgacagct acccggcgaa ccgtccgccg atccctttgc
      301 gggaaggata aaggccgctg gacatcttgt tctgtttgag ctgattttcc caaagtcacg
      361 attccgacgg gtgttctcgt gaaaattgca aacttttgag tgcttcatcc tcgctggtaa
      421 aaatgggaat ccattcatct atttccgtga gttccaatat atcgcggaca aaccctagcg
      481 gggcaatgat tcccagcctg ccgccaatcc gttgaaaacg tcgttgggca agaactaacc
      541 atcccagggc agagctgtcg ataaatgaga ccgccaccat attcaaaatg acaaatcgaa
      601 acccttgtgt aatagcataa tcaatccgcc atttaaaatc ctgtcgggca tgctgatcaa
      661 aatgtcccgc aaaagtttca acgagagtat cgtcactcag actacgttcg gaaacgacca
      721 taaaggattc tttcaggtag gtattcagat ggtatatgtc ggtatcggtg ttccgggtta
      781 cacacttaac ccgccaattc ggcaaaaagc actctcagag gttctggaac ttcacccgac
      841 acaaccaggt gtctgatttt cgagggatta gaagccttaa gatcgaaaat tcggttggtg
      901 gggattcggg aagcagaagg ttccttatca tggccaacat ctattgcctt tcctggtttt
      961 ttttcgattt acaggcctga tagaataaaa agtttgagga ggaccactac aagcatttag
     1021 caaggtccac ttcatgtttt ttttgactta gcgggaaata cattcccccc acaaagggga
     1081 cttctcccgt tctcgggtat acgggcacga ttgaaaggcc gcgggattat gaatacacag
     1141 ttcaatggtc tctttgtttc ttttggagtg atcgcaggaa tgttctggat gatgtgtttc
     1201 gctttaccag gatgggctca acaacaaaat ggagaacatc cggctcatcc cctcgcttca
     1261 gccaacacac tgctggtaga aattgtcgcc ctttcggaac gaggcctgga gaattcttct
     1321 gctatcacca aaactattac agacaaatta agtgaagctg gttttaccgt ggtttcttct
     1381 ccggatcaac ctcatgaagc ggtcatacgt gtgaaatgcg aagaacggca gacatggacc
     1441 ggcccgagta aatatcgcag catcagtcct gtccccactt ctcgattatg gaaaggccct
     1501 gcctgtcata tttcctatcg ctatcaagat tcatctcctc aatggaagtg ggaagttcgc
     1561 acttcgtttg aagatatcgg cgcggctgcg gaggcccaag gagcctccaa taccgggacc
     1621 tttgcgctga atgcccttaa caccgaacta caacaggatg attttccttt gtttatcctg
     1681 gctgaatggg aacaaaccga tcggttgctg atgctcctac agaaatcaca gggcaacagt
     1741 gaacgacaaa tgactatcct ccaacttttg ggtacgatat cgtccgacaa agccttcaac
     1801 gctcttcaga atgccctttc ccatccgcct ttaatacccg cggcgttgtt ggcactcggc
     1861 caacaaggag aaacggcgat accaacctta atcaccttct tagaagcaac cctcaattcc
     1921 gaacatcgac tgaccgccat tcaggcattg gggtccattg ccacctatag caaagatcca
     1981 atgttgttca cgcaattcat acagttatta gactcagagg accccaaggt tcaaaccgaa
     2041 gccgtgaagg gattaggcaa tctgggcgat cgacgcgcca ttcaacctct ggaaaaactt
     2101 aacctgcaag cctggactaa tccttccagc agtcctgata tgcgtgctct tcgggaaatg
     2161 ttaagttcga gcctgtggca actcaggccg gacgcccaca cagggggatg accctttttc
     2221 tatcaccgtg gcattcgcat tcaagttctt ccttatcctg ccaaatcctg tttacgggcc
     2281 atgccctctc ctcgatttca cctttacttt tcagatcatc caggcatagg gttttaatga
     2341 aaaaatagtg tggtggagcc tgaggctgga cgatgcatcg aaacaaacgc cgatcccaac
     2401 acccttctat acgaaaaccc gggaatagtt tcctgccccc tgaagtccat cggctcttct
     2461 tgcaggccgt ggctcatcat caggccaaac gcttctcaca agccgaagat cagtacaacc
     2521 ggatcctcac tcaattcccc tctcacgcgg atactctcca tcttcgagga ttattggcct
     2581 atcaacaggg gcagaactct ctggcattga cttacatcaa gcaagcgatt gctgaagatt
     2641 ccggtaagcc tcactatccc tacaatctgg gtctggtgtt tgaaaaagaa gagcgatggg
     2701 aagaggccat tgcagcgtat cagcaagctc tcatagtcaa tccacaatat gtcgaagccc
     2761 tggccaatct ggggaatgtc tacagacgcc agcggcaatg gattgatgcc gtcactaccc
     2821 tccaacaggc gttgcaactt aaacctcaat ccgcagacct ccataacgcc cttggagtca
     2881 cctacaaaga acaaggtgaa cgagagaacg cgttgaggca ttaccaacag gcacttcaat
     2941 tatcgcctca ccatgcagaa gccctgaata acatgggtgt ggtgctccag gaccagggac
     3001 aactggacaa ggccgcagaa gcatttcaaa aagcgttaac agtgaaaccg gaatatgcca
     3061 atgcccatta tcatctggga ttgattcgtc tctggcaggg aaaggagtcc caggccttgg
     3121 ccggttttca ccgctcgggt gatctgacgc acaatcatgg tcatggagtt gctccgcttt
     3181 ttgtcacgaa agctcgagtg aaacatgatt tcgaacaact acagtatctg actgcacagg
     3241 tttccagcag gtcaatttcg aatgattatc gccatgccct gaccaatgtc tatcagcgat
     3301 tatcccaatc gtctccagac tccatgttca tgaaactctc agtccaagaa caaagccaac
     3361 ttgccccttc cttcaaccgg cttctctata ttcgcccaac gtcgcgcctg gatgggtacg
     3421 ccatcaatcc acaacttgat gtatcagcca ttgagacgga atatttttcc accacacccg
     3481 aagtcatgtc tgtagatggc ttgctcaccg aaaaggcgtt atcggaaatt cgcgcgtttt
     3541 gcttggaatc cacgatctgg aaacgtgatt atcccaacgg atatatcggg acctttttag
     3601 caaatggctt tgcctgtccc ctgctcttgc aaattgcgga agaactacgg acccgtttcc
     3661 ccaaaatttt tcagcactac caattgcaac aggcctgggc atttaaacat gacagtgccc
     3721 tgcgagggct gaacatgcat gccgatgcag cagccgtgaa tgtgaatttc tggattaccc
     3781 ccaatgaggc gaatcgcaat ccggaatcag gcgggcttgt ggtttggaat aaggaagcac
     3841 cggacgactg ggattttgtt gaatacaaca acgacaagaa caagcacaag attcaaactt
     3901 ttttggaaga aagcggagcg caacctatca atattccgca tcggcaaaac cgggccgtaa
     3961 tcttcaattc aaacctgttc caccaaaccg atatcatcga atttcaagat atctacgaat
     4021 gccgccggat caacattaca cttctgtacg gccatcgcca acaatcccgg caaagtggag
     4081 caacaaggca aacaaaaaag gatgagcgaa gagttctaaa aaagaatgat tagccatttg
     4141 gcgcgcccga caggagtcga acctgtaacc tcttgatccg tagtcaagtg ctctatccat
     4201 tgagctacga gcgcaatatg tcggtgactg agtgtagtgg ctcgatcggt gatgcggcac
     4261 cgaattcgaa ggcgactaca gcgccca
//
LOCUS       DATIAL010000198        14345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1434054, whole genome shotgun sequence.
ACCESSION   DATIAL010000198 DATIAL010000000
VERSION     DATIAL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434054"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VLA60_06210"
     CDS             <1..1275
                     /locus_tag="VLA60_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HSF08988.1"
                     /translation="EQEAWESRLKNLPGKAQIAQYEERVRNLKKAGKGGLEGLLGGVG
                     EVQALKQDIEQEIESLTGAKKEFENTMALLRTRLKQIQTAPQRDIQQLKDKYNLSPQG
                     LANMSQTLLGPQIGSWVHQGAAWYERAKPLLEGARTVGGEDGPQVHKPLRGKGLDVHF
                     KEAHPLPDFLIRLTKVSVDLDLGSLAGTVHNITTDQPTLGQPTTFAFSGDRLKGVQSV
                     LLEGALNHVEPADSKDQFTVQAKGYELTDLALSKDAKWPVTLTSGMSDINVNTELKGQ
                     ALTVHGAGNFRGLHLSAGREDDPNPLTKALSSAVTGISQLSLQADVTGTLEQPDVTVS
                     SDLDRILQDAAGKMVNELAAKFGAELQSAISAKIAEPMQQLKNNLSGFERIAGELTER
                     VTQHNDVLNSLLEKSLPKKGLKDLPGGFKLPF"
     gene            complement(1306..2724)
                     /gene="sthA"
                     /locus_tag="VLA60_06215"
     CDS             complement(1306..2724)
                     /gene="sthA"
                     /locus_tag="VLA60_06215"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HSF08989.1"
                     /translation="MSSRITTPSPFQYDLICIGSGPAGQRAAIQAAKVGKRVAVIEKK
                     HAVGGACLETGTIPSKTFREAVLSFTGPQQHWGRLPETAKTRPTAEQLITRVAEVIRI
                     EGEVINDQLRRNDVEFFQGEASFVDPHTLLVHTDQHSQTLSSAHILIAVGTIPTPPPN
                     IAIDGQYILDSDSIIHLEQLPRTMTVVGAGIIGIEYASMLGALGIEVTLVDSRIRPLE
                     FLDGEIVDELIYQMRNHQVTFRLGETVEKLEIARTPSSKVVIHLASGKRLVSGLVLYS
                     VGRTGATSTLQLEKAGLTVDARGRLQVDSHYRTTVPHILAAGDVIGFPSLAATSSEQG
                     RLAACYAFDMEATPMTSLFPVGIYAIPEISMVGATEEQLTLEKVPYEVGVARYREIAR
                     GTIMGDPNGMLKLLFHRNDHRLLGVHVIGTGATELIHIGQAILHTERGLEYFLTTVFN
                     YPTLAECYKVAALDAYNKLCEI"
     gene            complement(3010..3975)
                     /locus_tag="VLA60_06220"
     CDS             complement(3010..3975)
                     /locus_tag="VLA60_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSF08990.1"
                     /translation="MTHHHDQEAFAKLVDRIGPDHAGQRMEMQAHYSALLLKGYHLHI
                     HMEEFPAIAWLIKTLLKTTGLWPLAVKNTSQYRIVEHTVPIPHLPESFDGFRILHLSD
                     LHIEGMIDKGQALQTALSTLRYDLCVMTGDFRFLTYGHYDKTLTLMESLVSTIQAPYG
                     VTGILGNHDWLEMVPGLERCGIRMLLNEAQSLEKGSDAIWLLGLDDVHYYETGDLEKT
                     IRLAPTDAARILLVHSPEIIPEASTAGMDLYLCGHSHGGQICLPGSKPIITHCRCPRA
                     YKAGPWQYHSMRGYTSRGVGTSLFPIRLFCFPEIIIHTLTRAPKH"
     gene            4008..4421
                     /locus_tag="VLA60_06225"
     CDS             4008..4421
                     /locus_tag="VLA60_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08991.1"
                     /translation="MAIMQRLDLINPELPDIQFVLMVLALCTADLETMNAPPSVRETV
                     FNRCWVLMHESPPPVKTQERVLDLTLSDEITLEALVHVIRQTFEEHGFVELTWDHPPS
                     EPTRESTPEARPLVERLEQWEPPPDNLNPPASSSN"
     gene            complement(4699..5688)
                     /locus_tag="VLA60_06230"
     CDS             complement(4699..5688)
                     /locus_tag="VLA60_06230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HSF08992.1"
                     /translation="MREYLDFEKPLKELEERIAKLVKSKSKKVSVQEAIVKSTEQLAN
                     LEREIYANLSPWQHSQIARHPQRPSISDYLEHMTNGFMELHGDRLFGDDRAIIGGFAT
                     WKGRSIMAIGHEKGKTLKERMRRNFGMAKPEGYRKALRLMKLAERFNRPIITFIDTPG
                     AYPGVDAEQRGQAEAIARNLLEMSRLQVPILAVVTGEGGSGGALALGVADRVLMLEHA
                     IYSVISPEGCAAILWGDATKSSDAATALRMTGPELLKLNVIEEVIPEPLGGAHRDAKL
                     MADRVSQALDTHFLQLQKLSPAALRKQRETKFRSLGTFGSSQATPKQVKMVNS"
     gene            complement(5714..9178)
                     /gene="dnaE"
                     /locus_tag="VLA60_06235"
     CDS             complement(5714..9178)
                     /gene="dnaE"
                     /locus_tag="VLA60_06235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HSF08993.1"
                     /translation="MPAQFVHLHLHTQYSLLDGANQLTPLFQQVKNFGMPAVAMTDHG
                     NLFGAIDFYQKAKAHDVKPIIGCEAYMAPGHRTQRAGHLAHNEYFHLILLATNQVGYH
                     NLIKLSSKAYLEGFYYKPRMDKELLQEHQEGLIALSGCLSGEVPQLINQQDMAGALRV
                     ADEYRSIFGKDRYYLEVQANGLDHQLIANRGLLEIHEKLGIPLVGTNDCHYLNKRDAR
                     PHEIMLCLQTGKTLKDPNRMKFDTDQLYVKSTEEMIAEFAEFNRAVLNTTQVAEQCDL
                     QLEFGTSYLPDYHVPEGQTHNSYLQHLAEEGLRTRLRERPTGIPSEAYQQRLHTELAV
                     LNAMGYAGYFLVVWDIINFARSRNIPVGPGRGSAAGSLVAYALRITDLDPLTYNLLFE
                     RFLNPERVTMPDIDMDFCMDRRGEVINYVIEKYGEAHVCQIITFGTLGAKAAIRDVGR
                     VMDFPYAEVDRVAKLVPTQLNITLQDALKQEPRLQELVDQDAKMKELMDTAMALEGLA
                     RHASTHAAGVVISQKPLMDHVPLYKTSNDEIVTQYTMTDLEKVGLVKFDFLGLKTLTM
                     IHDAVRMVNAQHPDEPPLDINNLPLNDLQTFELLSSGKTSGIFQLESSGMRNLLVKIK
                     PETFEDLIAILALYRPGPLESGMVDDFIKRKRDASQIVYDPPQLEPILKETYGVIVYQ
                     EQVMAVANQLAGFSLGQADLLRRAMGKKKHEEMAKQKELFVSGAKGKGISEKLSEKLF
                     DQMAFFAGYGFNKSHSAAYAVVTYQTAYLKAHFPTEFMAALLTSEMGNTDKMVGYFTE
                     CRELGVHILPPDANQSLKNFSVVPEGIRFGLVAIKNVGGNAVDVILESRDREGPFSSF
                     IDFCCRIDSQKVNKRVLEGLIKVGAFDSMGLTRASVYKVLDEALDHASTVQRNKREGQ
                     TSLFEILAPEPAAPKQEGFGFVIPQIPEWSQNDLLKYERELTGFYITAHPLNRHSVGI
                     THFSTCSTQTIRDVGDGREVKVCGVISSLKITTTKKGHRMAYAQLEDLHGTVEAIIFP
                     ETFKQHEELLGPESVVRITGTVDLMDNGARIKATKVESLSQLENETVKHVTLKVHEQD
                     VSPHNLLDLKDIFERHRGPTPISLAFHLHPNLTASMSGLSDIGISPSPEFLEEVEHLL
                     GASTVTFQ"
     gene            9678..9998
                     /locus_tag="VLA60_06240"
     CDS             9678..9998
                     /locus_tag="VLA60_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HSF08994.1"
                     /translation="MILTTTPNVEGHKIKDYLGVVAGEAILGTNFFRDFFANIRDIVG
                     GRSGAYEKELRRAREIAFQEIQEEALRVGANAIVGIDLDYEVMGETGSMLMVSVSGTA
                     VRVE"
     gene            10222..10695
                     /gene="bfr"
                     /locus_tag="VLA60_06245"
     CDS             10222..10695
                     /gene="bfr"
                     /locus_tag="VLA60_06245"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HSF08995.1"
                     /translation="MKAKKGVIDFLNKILTNELTAINQYFLHGEMCGHWGYEQLHNEI
                     RKHSIDEMKHAEELIEHILYLDGVPNLQKLGTLKIGETVPEQFKSDLALEHEAVVLLT
                     EAITHCASVGDYTTRHKLEEIVKSEEEHIDWIETQMETIKQVGVENYLAQHMHEK"
     gene            complement(10854..12920)
                     /locus_tag="VLA60_06250"
     CDS             complement(10854..12920)
                     /locus_tag="VLA60_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSF08996.1"
                     /translation="MRHLHALAVCFPTYILFSLLWTGSASGHDQEEVTVFEEVQVVGE
                     RPIAASSNRIILNEDILLQPQGRPADLLRLAPGLITLEHSGGAGKADQFLLRGFDADH
                     GSDLALHVDGMPINMRSHAHGQGYGDLNFIIPETIEEITVKKGPYHVEYGDFATAGAA
                     NYVTRESVPQTMVQSAGGNFNTQRHLFMTSPTHDRFRTLFAGEFYYTDGPYDFVNRNT
                     RYNGLAKLTFDPSATSQLSVTLSQYYGRWNGSGQLPLRDVRSGGVDRFGSLDPSEGGK
                     SLRSTGRLDYHYDLSGGGTVFANLWAQYYYLSLFSNFTFYLNDPVNGDGIEQTDRRWL
                     TGSDVGYRQIFRLFNYEGTMTAGLQTRFDQIQVRLGTQQKRSSLAITQESDIFEASYS
                     PYLKLDLQFLPWMRFVGGGRVDVFTYNVKDRCGADCSERPNGTASNAIASGKANLVLG
                     PWYQTEFFLNIGTGFHSNDARDAVENPSTNSLTRAIGYEIGIRSRPWTWSEFLATFWL
                     LDLGSELVFVGDEGTTEPRGKTRRLGTEFSSRITPMEWLTVRGDITYTHAEFRKTGDS
                     VPLAPQFTAFSSVTARFPIGLSGTLQMLTVGSRAGTEDASVKLEPFTIFDLVLRYKIP
                     LAPPTGRLEAFFSIRNLTDTDWRQAQFYYESRLPGEPAGGVADIHFVPGTPRMFMGGL
                     TWFWST"
     gene            complement(13130..13546)
                     /gene="nikR"
                     /locus_tag="VLA60_06255"
     CDS             complement(13130..13546)
                     /gene="nikR"
                     /locus_tag="VLA60_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HSF08997.1"
                     /translation="MSQLVRFGVSLEETLLSAFDQHINRKGYQNRSEAIRDLIRNQLV
                     GEEWGGNKETVGTITLVYDHHQSDVLQQLTHHQHEYGKQIISNLHVHLDHDHCLEVIV
                     VKGRSSALVDLANLLIKTKGVKHGQLTMTTTGKAVK"
     gene            complement(13735..>14345)
                     /locus_tag="VLA60_06260"
     CDS             complement(13735..>14345)
                     /locus_tag="VLA60_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF08998.1"
                     /translation="SGSIAQTVKQGMVWGLGHTLTLLIFSSLVLMLGSIIPERFAAGL
                     ELMVGLMLIGLGLDVFRRLQKNRIHFHVHQHPDRPPHIHAHAHTEPTHPDRTSHDHAH
                     GFPYRALLIGLMHGMAGSAALILLTLNATLSMSQAIMYILLFGLGSILGMGLLSVIMA
                     IPFWYSARSLTWLHNGLQACVAVMTTILGIVMAFSSGSSLWH"
BASE COUNT         3577 a         3351 c         3878 g         3539 t
ORIGIN      
        1 gagcaagagg cctgggaatc acgactcaag aacttaccgg gaaaagccca gatcgcccaa
       61 tatgaggaac gtgtaagaaa tttaaaaaaa gcggggaagg ggggattgga gggcttgctg
      121 ggaggggttg gcgaagtgca agcgttgaag caggatattg aacaggaaat tgaatcgtta
      181 acaggtgcca aaaaagaatt tgaaaatact atggcgttgt tacgtacccg gttgaagcag
      241 attcaaaccg caccgcaacg ggatattcaa caactcaagg acaaatataa tttgtctccg
      301 caaggattgg caaatatgag ccaaacgcta ttagggcctc agattggatc gtgggttcac
      361 cagggggcgg cttggtatga gcgagcgaag ccgcttctgg aaggagcacg gacagtcgga
      421 ggggaggatg gtcctcaggt gcacaagccc ttgcggggga agggcctgga tgtgcatttc
      481 aaggaagcgc atccgctccc ggactttcta attcgtttga cgaaggtatc ggtcgattta
      541 gacttaggga gtttagcggg gactgttcac aatatcacca ccgaccaacc gactttggga
      601 caacccacga cattcgcctt ttccggggac cgattgaaag gggtccaatc tgttttattg
      661 gaaggggctc tcaaccacgt ggagcccgcc gattctaagg atcagtttac ggtgcaggcg
      721 aaaggatatg aactcaccga tctcgcattg tccaaagatg ccaaatggcc ggttactctg
      781 acgagcggga tgagcgatat caatgtgaac acggaactca agggccaggc cttgacggtt
      841 catggcgccg ggaattttcg tggccttcac ctttccgcag gaagggagga tgatcccaat
      901 cctttgacca aagccctcag ctctgccgtg actggtattt cccaattgtc tcttcaggcc
      961 gatgtcacgg ggacattgga gcaacctgat gtcacggtat cctccgacct ggatcgtatt
     1021 cttcaggatg cggctggcaa gatggtgaat gaattggctg cgaagtttgg tgctgagctt
     1081 caatcggcca tctcggcgaa aattgcagag ccgatgcaac aactcaagaa taatttatcc
     1141 gggtttgaac gcattgccgg tgagttgacg gagcgggtca cgcagcataa tgatgtgctc
     1201 aacagtctcc tggaaaaaag tcttcccaag aaaggtctaa aggatcttcc cggcgggttc
     1261 aaacttccgt tttaactatg acttaactgt gacctcaaat gtgtattata tttcacaaag
     1321 cttattgtaa gcgtccaagg ctgcaacttt gtagcattcc gctagcgtcg gataattgaa
     1381 gactgtcgtg aggaagtatt cgagcccccg ttcggtatga aggatggcct gtccgatgtg
     1441 aatcagctcg gtggctcccg tcccgatcac atgcacaccc aatagccggt gatcatttcg
     1501 atgaaataac agtttcaaca tgccgtttgg atctcccatg atggtgcccc ttgcgatctc
     1561 gcggtaacgg gcgactccca cctcatacgg aactttttcg agcgtcagtt gctcctccgt
     1621 agctcccacc atagaaattt ccggaatggc ataaatacca acaggaaaca ggctcgtcat
     1681 cggtgtggct tccatgtcga aggcatagca ggccgctaaa cggccttgct cggaagatgt
     1741 ggccgccagg cttggaaagc cgatgacatc gcctgcggcc agaatatgcg ggacagtcgt
     1801 gcgatagtgc gaatcgactt gcagacggcc acgggcatcc acggtcaatc cggctttctc
     1861 aagttggagt gtcgatgtgg ctcccgttcg tccgacggaa tagaggacca ggcctgaaac
     1921 cagtcgtttt cccgaggcca aatggatcac gacttttgaa gatggtgtcc tcgcgatttc
     1981 caatttttca acagtttcac ccaggcgaaa agtaacctga tgatttcgca tctgataaat
     2041 gagttcatcg acaatttccc catccagaaa ttccaacggt cgaattcgtg aatccacgag
     2101 ggtcacttcg atgccaagtg cgcctaacat tgaggcatat tcaatgccga tgatcccggc
     2161 tccaaccaca gtcatggttc gtgggagctg ttccagatgg atgatgctat cactatcgag
     2221 gatatattgg ccgtcaatgg caatgttggg tggaggcgtt ggaatggttc ccacagcaat
     2281 cagaatgtgg gcgctcgaca gggtttgtga atgttggtcg gtgtgtacca acagggtgtg
     2341 gggatccaca aaggaggctt ctccttggaa gaattccaca tcgtttcttc gtaattgatc
     2401 gttgatcacc tcaccttcga tgcgaatgac ttctgccacg cgagtgatca gttgctcggc
     2461 cgtgggtcgt gttttcgcgg tttccggtaa acgaccccaa tgttgttggg gtccggtaaa
     2521 ggacaagacg gcttcacgaa aggttttact aggaatggtt ccggtctcta aacaggcacc
     2581 gccgacggca tgcttctttt caataaccgc cacgcgtttt cctaccttgg ctgcttgaat
     2641 ggctgcccgt tgtccagccg gtccgctacc gatacaaatc aggtcgtatt gaaatggtga
     2701 gggtgtcgta atccgggagc tcatggtgat agaacgaagg aggatgccaa aacagatgaa
     2761 cgctcagagg atagcgtggc aaaagagaga agtacaggtc aagctacttc tggatactat
     2821 agccgcatga ttgctgctgt ggttaataag actgtgtgag gagtcggtcc gcccgtgtat
     2881 ggattcatgg ccggcggaaa tctggtttta aaaaggaaaa aatcttaggc atgtgtatgg
     2941 gctgtccatc tttacggatt ctttccggga tgtcgaggag cctccgtgtg agtgggggtt
     3001 aggcgacgct caatgtttag gggctcgtgt gagcgtatgg atgatgattt ccggaaagca
     3061 gaacagccga atgggaaaca gggaggtgcc gactccacga gaggtgtacc cgcgcatgga
     3121 atgatattgc caggggcctg ccttgtaggc cctggggcag cggcaatggg taatgatggg
     3181 tttgcttcct ggaaggcaga tttggcctcc gtgtgagtgg ccgcagaggt acagatccat
     3241 tccggccgtc gaggcctcag ggataatctc aggggaatgc actaggagaa ttcgtgcggc
     3301 gtcggtgggc gcgaggcgta tggttttttc caggtcaccg gtttcgtaat agtggacatc
     3361 gtctaatccc aggagccaga tcgcatccga tcccttttcc agggattgag cttcattgag
     3421 cagcatgcgg attccgcacc gctcaagtcc gggcaccatt tccagccaat cgtgatttcc
     3481 caaaatgccc gtgacgccat agggagcctg gatcgtgctg accaatgact ccatgagtgt
     3541 cagtgtcttg tcataatggc cataggtgag aaagcgaaaa tcgccggtca tgacgcacag
     3601 gtcgtatcgc aaggtggaca gagctgtctg aagcgcttgg cctttatcga tcatcccttc
     3661 gatatgaaga tctgacaaat gcagaattcg aaatccatca aaggattctg gcaggtgagg
     3721 aattgggacg gtgtgctcga caattcgata ttgggaggta tttttgacag ccagcggcca
     3781 aaggccggtt gtttttaaaa gtgttttgat gagccaggca atggctggaa attcctccat
     3841 gtgaatgtgg aggtgatagc cttttaacag gagggccgaa tagtgggcct gcatttccat
     3901 gcgttggcct gcatgatcag gcccgattcg gtccactaat ttggcgaagg cttcttggtc
     3961 gtggtgatgg gtcatgtgct tgtcagccaa gtgggatcag aggtagcatg gccattatgc
     4021 aacgattaga tctcattaat cccgagttgc ccgatatcca atttgtcctc atggtattgg
     4081 cattatgtac ggcagacctc gaaacaatga atgccccacc ttctgtgaga gagacggtct
     4141 tcaatcggtg ttgggtatta atgcatgaaa gccctccgcc tgtgaagaca caagagcggg
     4201 tcctggattt gaccctgagt gatgaaatca cgctggaggc cttggtccat gtcatccgac
     4261 agaccttcga ggagcatggc tttgttgaat tgacctggga tcatccacca agcgaaccga
     4321 cacgtgaatc gactccggaa gcccgccctt tggtcgagcg gttagaacag tgggaacctc
     4381 ctccggacaa tttgaatcct ccagcatctt catctaacta atcctctatc ctcacagttg
     4441 agggtttctt tggccggttc tccgtcctga tagatggaga tcctctctgc tcagcgaagg
     4501 atcatcctgg gggctcgggt gcgcaataca tagtggccgt tgcctggagc acaaagataa
     4561 tgggactgcg aaacgtgatt ctcgtgatac ctaatggtgg gggtatgaac aaccgcctgg
     4621 agaacgggcg aacaactcaa aaggaaatta tcctgtgggg tcttggtcgc gtaaacccgt
     4681 ttacggactt tctgcaggtc atgagttgac cattttcacc tgtttgggtg tggcctgaga
     4741 agaaccaaag gtacccagac tccggaattt agtctctcgc tgctttcgga gtgcggcggg
     4801 cgacaatttc tgtaattgca ggaaatgggt gtcgagtgcc tgggagaccc ggtcggccat
     4861 gagtttcgca tcacgatggg ctcctcccag gggttcaggt ataacctctt cgatgacatt
     4921 aagtttgagt aactccggtc ctgtcattcg taaggccgtt gcggcatcgg aggattttgt
     4981 ggcgtctccc cacaaaatgg cggcgcagcc ttctggggaa atcaccgaat aaatggcatg
     5041 ttcgagcatc agaacccgat ctgccacacc taaggccaac gcgccaccac tgccgccttc
     5101 tcctgtgacc acggcaagaa tcggcacttg caatctggac atttcaagta aatttctggc
     5161 aatggcctcg gcttggccgc gctgctccgc atcgacgccg gggtatgctc ctggagtatc
     5221 gataaaggta ataattggtc gattgaagcg ttccgctaat ttcatgagac gcaaggcttt
     5281 tcgatagccc tccggtttgg ccataccaaa atttcggcgc atgcgttctt ttaaggtctt
     5341 tcctttttca tgaccgatag ccatgatgga gcgacccttc catgtggcaa accctccaat
     5401 gatcgcccgg tcgtcgccga acaggcggtc accatggagt tccataaacc cattcgtcat
     5461 gtgctccagg taatcagaaa tcgaagggcg ctgtgggtgc ctggcgattt gactatgttg
     5521 ccatggggaa agattcgcat agatctcacg ttcaagattt gccagttgtt cggtcgactt
     5581 gacgatggcc tcctgaaccg agaccttttt ggattttgat ttcactaact tggcgatgcg
     5641 ttcttcaagt tccttcaggg gtttttcaaa gtccaggtat tcacgcacgg acaagctcct
     5701 tggtagaggg aaattattgg aaggtgaccg tggatgcgcc gaggagatgt tccacttctt
     5761 ctaaaaactc cggtgacgga gaaataccga tatctgataa tcctgacatg ctggccgtca
     5821 gattaggatg caagtgaaac gccaacgaaa taggggtcgg accgcggtgg cgttcgaata
     5881 tgtcctttaa gtctagcaga ttgtgggggg aaacgtcctg ttcgtggacc ttaagcgtca
     5941 catgcttcac cgtttcattt tctaattggg agagggattc gactttggtg gctttgatac
     6001 gggccccatt atccatcagg tccacggttc cggtaatccg aaccaccgat tccggcccca
     6061 ataattcttc gtgttgctta aaggtttcgg ggaaaataat cgcttcaacc gttccgtgca
     6121 agtcttccaa ttgggcgtaa gccatcctgt ggcctttttt tgtcgtcgta attttgagac
     6181 tggaaatgac cccgcagact ttgacctctc gtccgtcgcc gacgtcgcga atggtctggg
     6241 tggagcaggt agaaaaatga gtgatcccaa cgctgtggcg gttgagagga tgcgcggtga
     6301 tataaaagcc ggtcagctct cgttcatatt tgagtaagtc attctgcgac cactcgggaa
     6361 tctgtggaat gacgaaacca aaaccttcct gcttgggggc tgccggctcg ggagccagta
     6421 tttcaaacag actggtttgc ccttcccgtt tgttgcgttg gaccgtcgag gcatggtcca
     6481 aggcttcatc caggacctta tacacgctgg cgcgtgtgag acccatggaa tcaaaggctc
     6541 cgaccttgat gagtccttcc agaacccgtt tgttgacctt ctgcgaatca atgcggcaac
     6601 aaaaatcaat aaaggaggaa aacgggcctt ccctgtctcg tgattctaaa atcacatcca
     6661 cggcgtttcc gccgacgttc ttgatcgcca ctaatccgaa ccggataccc tcaggtacga
     6721 cactgaagtt ttttaagctc tggttggcat cggggggcag gatgtgtacc cccaattcac
     6781 ggcattcggt aaaatagccg accatcttgt cggtatttcc catttcagat gtcagcaggg
     6841 cggccataaa ttcagtggga aaatgggctt tcaggtaagc cgtttggtat gtcacgaccg
     6901 cataggcagc ggagtgtgac ttgttaaatc cgtagcctgc gaaaaaggcc atttggtcga
     6961 ataatttttc agaaagtttt tccgaaattc ccttcccttt ggcacccgag acaaacaatt
     7021 ccttttgttt ggccatttcc tcatgttttt tcttgcccat cgcccgtcgg agtaaatcgg
     7081 cttgccccaa tgaaaatcct gccaactggt tggcgacggc catgacctgt tcctggtaga
     7141 cgattacccc ataggtttct ttgagaatgg gttccaattg cggaggatca tagacaattt
     7201 gggaggcgtc gcgttttcgc ttaatgaaat cgtccaccat gccgctttct aaggggccgg
     7261 gacgatagag cgcgaggatg gcgatcagat cttcaaacgt ttccggtttg attttaacca
     7321 acaggtttcg catgccggaa ctttccagtt ggaaaattcc tgaagttttt ccagagctga
     7381 gcagctcaaa cgtctggaga tcattcaagg gcagattgtt gatgtccaat ggcggctcat
     7441 caggatgttg agcattgacc atccgaactg cgtcatgaat catcgtcagg gttttgagcc
     7501 ccagaaaatc gaatttcacc aacccgactt tttccagatc ggtcatcgtg tattgggtca
     7561 cgatttcgtc gttacttgtt ttgtagaggg gcacatgatc catgaggggt ttttgcgaaa
     7621 tgaccacgcc tgccgcatga gtcgaggcat gtcgtgccaa gccttccaag gccatggccg
     7681 tgtccatgag ttccttcatt ttggcatctt gatcgacaag ttcctgcagg cgtggttcct
     7741 gtttcagggc atcctggagg gtgatgttga gttgggtggg aaccaacttc gcgacccggt
     7801 caacttctgc ataggggaag tccatcactc tccccacatc ccggatggcc gctttggcac
     7861 ctagtgtgcc aaaggtaatg atctggcaca catgcgcctc tccgtatttc tcaatgacat
     7921 agttgatcac ttccccacgg cggtccatgc aaaagtccat gtcgatatcg ggcatggtga
     7981 cacgttcggg gttaaggaag cgttcaaaga gcagattgta ggtgagggga tcgaggtccg
     8041 taattcgcag ggcgtacgcc acaaggcttc cggcggccga tcctcgcccg ggtccgaccg
     8101 gaatgttgcg ggatctggcg aaattgatga tatcccacac aaccaaaaaa tatccggcat
     8161 accccatggc gttcagcaca gcgagttcgg tgtggagtcg ctgttggtac gcctccgatg
     8221 gaatgcccgt tgggcgttct cgtaaacggg ttcgaaggcc ttcttccgca aggtgctgaa
     8281 ggtaggagtt atgagtctgg ccttcaggta catgataatc cggcagatag gaggttccga
     8341 actccaattg aagatcgcac tgttcggcca cctgggtggt attcaagacc gcccggttaa
     8401 attccgcaaa ttccgcgatc atttcttcgg tagatttcac atataactga tcggtatcaa
     8461 acttcatccg gttgggatct ttgagcgttt ttcccgtttg aaggcacaac atgatttcat
     8521 gcggacgcgc atcacgttta ttgagataat gacaatcgtt ggtgcctacc agcggaatgc
     8581 caagcttttc gtgtatctcc agtaatcccc gattcgcaat aagttgatgg tccaagccgt
     8641 tagcctggac ttccaggtaa tagcggtcct ttccaaaaat tgaccgatac tcatcggcca
     8701 cccggagagc tcctgccata tcctgttggt taatgagctg tggcacctct ccgctcaaac
     8761 aacctgacag ggcaatgaga ccttcctgat gttcctgtaa taattccttg tccatgcgag
     8821 gcttgtaata aaagccttca agataggcct tgctggagag tttaatgagg ttgtgatagc
     8881 cgacttgatt cgtggcaaga agaatcagat ggaaatattc gttatgcgca aggtgtcccg
     8941 cccgctgcgt gcggtggcct ggagccatat aggcttcaca cccgataatc ggtttgacat
     9001 catgagcttt ggctttttga taaaaatcaa tggcgccaaa gagattgcca tggtcggtca
     9061 tggcgacggc gggcataccg aaattcttca cctgttggaa caggggtgtc agttggttgg
     9121 ccccgtcgag gaggctatac tgggtgtgaa gatgaagatg aacgaattgt gctggcactc
     9181 atatggtcct tccgtaaaaa ttctaatgag cacctcgagt gaagtcaaat gaaaatccta
     9241 tgtgaaaaac cacgcggtgt ccggtttctg aattttttaa aaattgcaag aataaattga
     9301 agaaataata tggagaaatg aaaaaaagta atccagaaaa aatgggaaca agtatgtgga
     9361 taaatcgtga ggatccgatt gaaaggcaag ggagagaatg cgttcagcag attgcttatt
     9421 tggtgagcat agtagtgacc gatgaaacgc ctaccacggg ttgggtcaca aagatgttca
     9481 ggaaaagatc cggatttcag agatgaaatt tttcttcctg tattgatgat tcgcgaccct
     9541 ttgtcgcgct cccataactg acatacaaga actgtttgtg gaaaaattgt gatgggagca
     9601 cccacgttga acatatcatg gacatcaatc gatagagtat ttgaagcatt gcaaatcagg
     9661 taaaggaggg agaggacatg attctgacaa ctacgcccaa tgttgaaggc cataagatta
     9721 aagattattt aggtgttgtg gcaggcgaag caattttggg cacaaatttt tttagggact
     9781 tttttgccaa tattcgtgat attgttggcg gccgatcagg ggcctacgaa aaggaattgc
     9841 gacgcgcgcg tgaaattgct tttcaggaaa ttcaggaaga agccttaaga gtgggagcca
     9901 atgccattgt tggaattgat ctggattacg aagtcatggg ggaaaccggg agcatgctca
     9961 tggtgagtgt aagtgggact gccgtgaggg ttgaataagt gctgggtgac ggaagagtaa
    10021 attcatattc tccatggacg gccataattg aacttgaaaa tatagatcaa cgaagttgag
    10081 aaaggaattc aatattaatt aattttccct aaataaaaac attgacccgg ccgaagctgg
    10141 accgataaaa taatcaaagt aattgaagca gaacaagcaa tttctcacgt ctgagacaat
    10201 ttttaaaaaa ggagaacttc catgaaggca aaaaagggtg tgatcgattt tctcaataaa
    10261 attttgacga atgaactcac tgcgattaat caatattttc tgcatggcga aatgtgtgga
    10321 cattggggat atgaacaatt gcataatgaa attaggaaac attcgattga tgaaatgaag
    10381 catgcagaag aactgattga gcatattctt tatttggacg gtgttcccaa tctgcagaaa
    10441 ttgggtacgc tgaaaattgg agagacggtt cctgagcaat ttaaatcaga tttggctctt
    10501 gaacatgaag cggtggtcct tctcaccgag gcgattaccc attgtgccag cgtcggtgat
    10561 tacaccactc gacacaaatt agaagaaatt gtcaagagtg aagaggagca tatcgattgg
    10621 atcgaaacgc aaatggaaac cattaagcag gtgggagtag agaattatct ggctcaacac
    10681 atgcacgaaa aataagcgtt tctgttcctc cagataaaca gggctttccg ggattgccga
    10741 tgattcctgg aaagcccttt ttcatgggat cattgatgcc gggttttatc gtggagaagt
    10801 ctgctctggg ctgtctgatg cagaccttgt gaatgcggtc tttttcctgt ctgttatgtc
    10861 gaccaaaacc acgtcagccc acccatgaac atccgtggag tgccggggac aaagtgaata
    10921 tcggcaacac ctccggcggg ttctccggga agacgggact cataataaaa ctgggcctga
    10981 cgccaatcgg tatcggtcaa attgcggatg ctaaaaaatg cctccagccg tccggttggt
    11041 ggcgcaagag ggattttgta gcgaagaacc agatcaaaaa tcgtaaatgg ttctagtttc
    11101 acactggcat cttccgtgcc ggcacggctt cccaccgtca gcatttgcaa cgttcccgat
    11161 agtccaatcg ggaagcgggc tgtgacggat gaaaacgcgg tgaactgtgg cgccaaaggg
    11221 acggaatccc ccgtttttcg gaactcggca tgggtatacg tgatatctcc tcgaacggtg
    11281 agccactcca tcggggtgat tcgagagctg aattctgttc ccaggcgacg cgtctttcct
    11341 cgcggctccg tggtcccttc atcccccacg aataccagtt cagatccgag atccaaaagc
    11401 caaaatgtgg ccaggaattc ggaccaggtc cacgggcggc tcctaatacc gatttcataa
    11461 ccaatggctc gggttaagct gtttgtcgac ggattttcga cggcatcccg cgcatcattg
    11521 ctgtgaaaac cggttccaat attcaggaag aattccgtct gataccaggg acccaaaacg
    11581 agattggctt tcccgctggc gatggcatta gaggctgtcc cgtttggtcg ttccgagcag
    11641 tctgccccgc aacgatcttt aacattatac gtgaagacgt caacccggcc gcctcccaca
    11701 aatcgcatcc agggaaggaa ttgaaggtcc aacttcagat agggagaata ggaggcttcg
    11761 aaaatgtctg attcctgtgt gatcgccagg gaggagcgtt tctgttgcgt gcccaaacgc
    11821 acctggattt gatcgaagcg tgtctgaagt ccggccgtca tggtgccctc ataattgaac
    11881 aggcgaaaaa tctggcgata accgacatcg cttccggtca accagcgtcg gtcggtctgt
    11941 tcaatgccgt caccattcac cggatcgtta agataaaacg tgaagttgct aaacagggac
    12001 aggtaataat attgagccca cagattggcg aataccgtcc ctccgccgga tagatcgtaa
    12061 tggtaatcca atcgaccggt gctccgtaac gatttgccgc cttctgaagg atcaagagaa
    12121 ccaaagcggt ccacgccccc ggacctcacg tcccgcaagg gaagctggcc tgacccgttc
    12181 catcgtccat aatattggga aagcgtcaca ctgagttggg atgtggctga gggatcaaac
    12241 gtcagtttcg ccagtccgtt gtatcgggta ttgcgattga caaaatcata ggggccgtcg
    12301 gtgtaataaa attctcccgc aaaaagggtg cggaatctat cgtgagtggg agaggtcatg
    12361 aacagatgtc gttgggtatt gaaattccct ccggcggatt gcaccatggt ttggggaacc
    12421 gactcacggg tgacataatt ggcggcgccg gcggtggcaa agtccccata ctccacatgg
    12481 tagggtcctt ttttgacggt aatttcctca atggtttcgg ggataatgaa attcaagtcc
    12541 ccgtatcctt gcccatgcgc gtggctgcgc atgttgatcg gcatgccatc cacgtgaagg
    12601 gccaggtctg aaccatgatc ggcgtcaaag ccgcgcagaa gaaactgatc agccttcccg
    12661 gcgccgcctg aatgttccaa ggtaataagg ccgggagcca accgtaataa atccgcaggc
    12721 cggccttgag gttgaagcag gatatcctca ttgagaatga ttcgattcga agaagccgca
    12781 atagggcgtt ctccaacgac ctgcacctct tcaaaaaccg tgacctcctc ctgatcatgg
    12841 cctgatgccg aaccggtcca caagaggctg aagagaatgt aggtcggaaa acataccgcc
    12901 agcgcgtgga ggtgccgcat acccatggaa ttggtaaaga aaaaacgagc gtgggaacga
    12961 aggagaaact tgttggtaac acgaatataa aattagtagc acgactttta gcaaggtcgg
    13021 ctgatattgt caatccaaag tcgtgcggtc agggtaggga gaggagaaac atggggaaga
    13081 gaggaagcca tgcggtgtga cagaaatgtt ttgagaggag aaatgatccc tatttcacgg
    13141 cttttcccgt ggtcgtcatc gtgagttgtc catgcttgac ccctttggtt ttaatcagca
    13201 gattggctaa atcaacgagg gctgaactgc gccctttgac cacaatgact tccaggcaat
    13261 ggtcgtgatc caaatgcaca tgcagattcg aaatgatttg cttcccgtat tcgtgctgat
    13321 ggtgagttaa ttgttgcagc acatctgatt gatgatggtc atatacgagt gtaatggtgc
    13381 cgacggtctc tttatttccg ccccactctt cccctacgag ctgattacgg atgaggtctc
    13441 gaatcgcctc agagcgattc tggtatcctt tccggttaat gtgttgatca aaagcggaaa
    13501 ggagtgtttc ttcaagagaa accccaaagc ggaccagttg actcattgtc ctattccttc
    13561 cagtttggtt ttggctgtct cctatcctat ggtcttttct gtaaaaaaac aattgatgag
    13621 ccatacggga agaccggtcg gaacatcatg acgaccgacc ggcctaaaac cgaggagggt
    13681 gtgaagtctc gcgtttatga aggcgagttg cgtgaaggat cgagaaaaga cttgttaatg
    13741 ccagagtgag gatcctgagg aaaatgccat cacaatcccc agaatggtgg tcatcaccgc
    13801 cacgcaagcc tgaagcccgt tatggagcca tgtcagggat cgggctgagt accaaaaggg
    13861 aatagccatg atcacggaca ggaggcccat gccgagtatc gatcctaacc cgaaaaggag
    13921 gatatacata atggcttggc tcatggacag ggtggcgttc agagttaaga ggatcagcgc
    13981 ggctgatccg gccatcccgt gcatcaggcc gatcagcaac gcacgataag gaaaaccatg
    14041 agcatgatca tgggacgtac ggtcagggtg tgtgggttcg gtatgcgcat gcgcatgaat
    14101 gtggggaggg cgatccggat gctggtgaac atgaaaatga atccggtttt tttgtagtcg
    14161 tcggaacacg tcgagtccca ggcctatcaa catcaagccc accatgagtt ccaaccctgc
    14221 cgcgaatcgt tcgggaatga tgctgcccaa catcaaaacc aacgaactga agatcaacaa
    14281 cgtcagggta tgccccaatc cccagaccat cccctgtttg acggtttgcg cgatcgatcc
    14341 acttc
//
LOCUS       DATIAL010000199        21132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_896261, whole genome shotgun sequence.
ACCESSION   DATIAL010000199 DATIAL010000000
VERSION     DATIAL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 21132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21132
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896261"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VLA60_06265"
     CDS             <1..694
                     /locus_tag="VLA60_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HSF08999.1"
                     /translation="VRIILGCGMLCLALVTPISGWAQNASEEVFLVDKARFTLENFLA
                     DPDMQWFRDYMKDARGILIVPQFIKGAFFVGGSGGSGVLIARDEKTNEWSYPAFFTMG
                     GASFGFQFGGQASEVVLLVMTQKGIDSLMSTTLKLGADASVAVGPIGRGVEGSTAPNL
                     SADLLSFSKAKGLFAGISLEGAVVAARDALNTSYYKTPTKTIDILVRRSVNNPQANPL
                     LEVVKKATNGNN"
     gene            851..1600
                     /locus_tag="VLA60_06270"
     CDS             851..1600
                     /locus_tag="VLA60_06270"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSF09000.1"
                     /translation="MGDIKASDCRSEKPGFRQVVRLKRLGFSSVLAIVLVVAGCAQTE
                     QAGNVQTSGFIRDYSLLQEGGEGESLLVYKNPQADFSTYRTLYVDPVTVLLSKESSVP
                     QEQLHKLGGDLRSKMIWKLKEDFLVVPKLVPGALRLELALTEAVPADVGMDIITTLVP
                     PAGVISAATGLATGTRAFVGRATVEGKLTDGDTGTLLAAAVDRRMGGRTLDGSMDSWD
                     DVQQAFEYWATRLSQRLRDWRTTKEALPRSP"
     gene            1951..3399
                     /locus_tag="VLA60_06275"
     CDS             1951..3399
                     /locus_tag="VLA60_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HSF09001.1"
                     /translation="MGAAVQLHNRERAALLPIRSLANQAFSRAAGAPLIEGNTIRLLQ
                     DACENYPAWLDAIRGAKQYIHFENYIIHEDDAGRMFADVLISKAQEGVHVRLIYDWMG
                     GFGRASRAFWNRLRAGGVEVRCYNPPRWDSPFGWLSRDHRKTISVDGEVGFVTGLCVG
                     RMWIGVPEKKVEPWRDTGVEVRGPSVTVIEQAFADVWAMMGEPIPERDLVKPDVHAQK
                     GKTALRVVASVPATAGMFRVDLLMAALVRKRLWLVDAYYAGTTSYVQALRAAASDGVD
                     VRLLVPNATDIPLVRLLSRAGYRPLLEAGVRIFEWNGTMLHAKTAVADGHWARVGSTN
                     LNLASWLGNCEMDVVVEDEPFASLMEQTYLQDLENATEVVLDAKRKVRAPNQPRHPHP
                     IISSVGGSGGRAAAGALRLANTVGAVFTNRRVLEPLEARITVTAGVMLLTLTILFAFF
                     PQLLAFPLVLVFTWIAGALLYRGYKLYRGKAQ"
     gene            3918..4646
                     /locus_tag="VLA60_06280"
     CDS             3918..4646
                     /locus_tag="VLA60_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF09002.1"
                     /translation="MFGTIRNIEGEQIDYTFHPGDKSRKVIVVLGHGVTGNKDRPLLV
                     ALAEALSHDGIPTLRLSFSGNGDSEGRFEDSTISKEVEDLRCVLDALDGWKFLYVGHS
                     MGGAVGVLLAGKDTRLVGLVSLAGMVHTEAFAQREFGEVTPGVGFMWDEPACPLSQTF
                     IDDTAAIGTVVARAPSIRVPWLLVHGTEDDVVPLQDSLDIFERANKPKQLVRIEGADH
                     VFSEHIPDMIKVVHEWVREQIGRA"
     gene            complement(4796..5473)
                     /locus_tag="VLA60_06285"
     CDS             complement(4796..5473)
                     /locus_tag="VLA60_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HSF09003.1"
                     /translation="MPIKRMNFGYLLGGLLFLLLSVAIAREVGITSETRRLFLEPALC
                     LMLLMGIWSHVSEKKWLMVGGGILTVSGVATATIDYFLDIPELQFVNMAILLVFSLVS
                     TWIASRHLLLSGPITLNKIIGAICIYLLIGLNWSVFYLIINIINPHSFHGLTSTAIGV
                     QFSELLYYSYVTITTLGYGDVTPLRPIARTLSYLEAIIGQFYVAVLVAWLVGMYLSEK
                     NRTGTKP"
     gene            6134..6511
                     /locus_tag="VLA60_06290"
     CDS             6134..6511
                     /locus_tag="VLA60_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09004.1"
                     /translation="MTIRGKIDLKKRPGYGLQRTWSLILTILLAACAQNPAYLYEMGE
                     RPIPIGIEKEIIPQTTTQDEVLTLLGEPVARLKTQTHEGHIHIWTYSYLDLQSTSQDK
                     GESLTITFDDKTLVVQSVTRGPL"
     gene            7152..7763
                     /locus_tag="VLA60_06295"
     CDS             7152..7763
                     /locus_tag="VLA60_06295"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HSF09005.1"
                     /translation="MAALHTLIACHECDLLHRIHPLHYGERARCSRCGASLYAKKRDS
                     FEHTTILALASLIFFTLANVFPFMTFELNGRVQESLLSTGVREFFKREMWALGVLVLC
                     ASIIFPALKILGMLYVLGPLEFNKRPWHSALVFRMVEHCRTWAMMDVYLLGVIVAVVK
                     LSDLANLVPGVAIYSFVALILTLAAADSALDTHAVWEKMEKIS"
     gene            7760..8395
                     /locus_tag="VLA60_06300"
     CDS             7760..8395
                     /locus_tag="VLA60_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HSF09006.1"
                     /translation="MRASYPTAARSGLMSCHACHQLSRIPPMVNEVDAICPRCGAHLH
                     LRKPNSISRTWALLIAAYILYIPANLLPVMTVISFGKGEADTILSGVKELIHAGMLPI
                     ALLVFFASITVPVLKLLALTYLLLSVQYKSQWRPRQRTKLYRMTEVVGRWSMIDIFMI
                     SILIALVKLEAVATIEPGPGAISFAAVVILTMFAAMSFDPRLIWDNIKEKS"
     gene            8392..10053
                     /locus_tag="VLA60_06305"
     CDS             8392..10053
                     /locus_tag="VLA60_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSF09007.1"
                     /translation="MKDKESNPDDFEEFPEVTVQKKRGISIVWIIPIVAALIGGWLTY
                     KTISEKGPTVTITFKDGAGLEAGKTKIKYKSIEVGIVKTVHISHDLFHVVVTAELSKQ
                     AEPHLTENAQFWVVRPRLGLGGVSGLETLVSGAYIAMDPRPGASARTFTGLEKPPGVT
                     REDEGAQFQLQAENLGSSSPGAPVFYHDIQVGRVLDYVLDKEGEGVLIDIFIQAPHHL
                     RVRDTSRFWQLSGFEVSLGAEGLDVKMESLSSLLTGGIAFDNPANAGGSGEPSQPGTA
                     FKLFKNFSSIQDEKYVLTRPFVINFDGSVRGLSVGAPVEFRGIKIGVVSDIAVNLNPK
                     TLEIKIPVLIEIQPERITAPQLVKAQVLEDKYAIMKHMVKRGLRAQLETGSLLTGQLF
                     VGLDFHQNLPEKELIMTGKYPEIPAIPAAMDELRRTVTDVMAEIRRLPLDKIAREILE
                     TVEGGNRLVNSPDTQKAVHNLNAALGNVEKLTEGLDRQVDTLMTNLDNTLVMVQKGIR
                     QVDPNSPAAVNMNNALKELSAASRSIRVLADYLEQHPEALVKGKH"
     gene            10065..10697
                     /locus_tag="VLA60_06310"
     CDS             10065..10697
                     /locus_tag="VLA60_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HSF09008.1"
                     /translation="MKSIHGFRIIAACVILLTVLGCGTSQPSHFYLLRALSPSSVPGL
                     SDTKASGLSFGLGPVTLPKYLDRPQIVTQSGGHEVELAEFHKWAEPLSDNVSHVLAEN
                     LSVMLSTDRIEQYPWRRTTPVDFQIVVDVLQFDGTRGGEAVLSARWSLLGGDEQTVFT
                     TRKTHVTHHPTSQDYEALVEAMSHNLEDLSREIAEAIKVLLPTILPGSIS"
     gene            10749..11393
                     /locus_tag="VLA60_06315"
     CDS             10749..11393
                     /locus_tag="VLA60_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09009.1"
                     /translation="MSVVVMDCQKHTTQWHVAAGMFSDGHPDDGGNPIQRAEHDLHLP
                     GEQFMGMLSTVKHELKEVGLVTLYFFFCFGVILTLKKLMLEGYHIEFYAFSTALVGAL
                     IVAKVVGLLGKTRAGTRFDARYSLGMATLYRTIVFSGCTFLVLFAEKMFHAFRESDLL
                     GAAFMDVWAHKDLSLIFAKVMCIGLAFMGFFLYRGIDRRLGEGTLRRMVFDPPN"
     gene            complement(12509..13381)
                     /gene="atpG"
                     /locus_tag="VLA60_06320"
     CDS             complement(12509..13381)
                     /gene="atpG"
                     /locus_tag="VLA60_06320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HSF09010.1"
                     /translation="MANLQALRRKIGSVKNTQKITKAMKMVAASKLRRAQQRILAFRP
                     YGQELRRVLANLSSRVNQSVHPLFQKRPVKIIQVTVVTSDRGLCGAFNMNVIRKALQV
                     IQKCEETGAKVIVGVIGRKGNEYFRRRHWPIRDPNVNVFERLTYEHALEVGLAAVEAF
                     ADGVFDEGYIVYNEFKNVVQQQVVVEKVLPIEPLKEFGAAETQTGGGYLYEPDQEELL
                     STLLPKYAQGQVFRILLESSAAEQAARMAAMDGATRNAGELIKHLSLKYNRSRQEAIT
                     KELMDIVGGAEALK"
     gene            complement(13748..15259)
                     /locus_tag="VLA60_06325"
     CDS             complement(13748..15259)
                     /locus_tag="VLA60_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSF09011.1"
                     /translation="MHRSCVPILLSLLIAWGCTMGPDYKRPDVPTSDSWRLAPGTSES
                     IANMPWWELFKDEELQKLIRTALEENLDLRVAAAAVEEFNNQLVIAKFDFAPSLGYQG
                     EGMYYHNTNNGLSTGNGAVIPGQAGGQSGRGDLDFSRQSFVGGIKWEIDLWGRIRRNV
                     EATQAELLARVENQRGVVIALVSDVAQSYFTLRALDLQVQIAQRTLKIWNEAVRLSRS
                     QYEHGYASKLDLDRFEAEQAGTRAQLANLEQQVVQAENRISVLLGHRPNAIPRGLVLT
                     EQPLPPKVPIGLPSSLLSQRPDILEAEQKLVAATANIGVAQAQRFPQFSINAGGGVAG
                     FQLNSMGTGPFATAGASGTLTGPLLNATALGYGVGVQTAKEQQALAQYELAILNAFRE
                     VEDSLITIEKTHEQTEAQESQVASLKSALNFADKRYRGGFASYLDVLTAQRDLFQAEL
                     SLVSTRQQNLSSIVRLYKALGGGWSPPAKKGQPVSSPLEPGPMDQEMKPEQKI"
     gene            complement(15261..18497)
                     /locus_tag="VLA60_06330"
     CDS             complement(15261..18497)
                     /locus_tag="VLA60_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HSF09012.1"
                     /translation="MAKFFIDRPIVAIVISIIMTILGLIAMVQLPIALFPDIAPPEIQ
                     LQATYVGADAVTVEQSVATPIEQQMAGVDNSIYMYSTNASNGQMTLRVDFDVNTVPND
                     DQILTQMRYLQAESQLPSDVKNYGVTIKKSTTSPLMLFSLYSPNGTYDEIFLANYAYV
                     NINDPLSRSQGVGQVTIFGAGQYAMRFWVEPDHLAKLGITVTEILDAIKKQNTVNPAG
                     QIGAQPVPQGQEFTYTVRSQGRLLNEEEFGQIIVRADPDGSIIRLKDVARVELGAQSY
                     SMIGRFNGKPSALIAVYQLPGSNAIDTVDRAKKLMEEMKERFPEDLDYAISLDTTLAV
                     REGIKEIVHTLFEAVVLVIFVVFLFLQGWRATLIPLLAVPVSLVGTFAIFPLLDFSIN
                     TLSLFGLVLAIGLVVDDAIVVVEAVEHHIEKGLSPKDATRKAMEEVSGPVIAIALILA
                     AVFVPTAFIPGITGRLYQQFALTIALSVIISAFNALTLSPALSGMILRPRTPARGPLG
                     AFFGWFNHWFGRATDGYVGYCHHLIRKSGRAMLFLVVAAVCAGLLGAKLPTGFLPMED
                     QGYVYLNVQLPLAASLQRTDEVAKKIEAILSETPGIQHVSTIVGFSLLSTVNTTYNGF
                     FFVTLAPWEERTKPEEKLQAIFKNVNEKLAALPEANAFLFPPPAIPGVGTSGGVSFIL
                     EDRSGQDVAFLADNLNTFMEAARQRPEIASMNTTFIPAVPQVFAHVDRDKVLKQGVKL
                     EDVYKTLQTFMGGVMVNYFNRFGRVWQVYVQAEGEFRTKAENVGQFFVRNKSDEMVPL
                     STLVTMEKISGPEFTMRFNGYRSAQIFASANSGFTSGQVMQAMEEVFTETMPREMGYD
                     FIGMSFQEKVASEGVPASVIFGFSLLFVFLILAAQYESWGLPFSVLICTPIAVVGAFG
                     ALWALGMENDVFTQIGLVMLIGLSAKNAILIVEFAKMEYEKGRSLIDAALTGARLRLR
                     PILMTSFAFIFGVVPLVLSSGSGAHGRILLGVTVIGGMIAASFIAIFLIPVSFYVVER
                     LVHRHEDPPDQDPDGQEFEDSELLEDEESFVSPQTKHAQTQFKLQGGH"
     gene            complement(18508..19599)
                     /locus_tag="VLA60_06335"
     CDS             complement(18508..19599)
                     /locus_tag="VLA60_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF09013.1"
                     /translation="MPPEVLVAEVIQKDVPIYGEWIGTTVGYVDAQIRARIQGYLISR
                     HYTEGSVVKANDLLFKIDPRPYKAALDQAKAELARTEAAYKKSQLDVNRNTPLAAEGA
                     ISQKELDDAIQASAANLASVASARAKLENAQLNLNWTNVTSPINGIAGIATAQIGDLI
                     VENTLMTTVSQLDPIKVNFPISEREYLKLAERIEKAGGQEKLNPAHEKRLELVLADGS
                     IYPHKGRAVLADRQVDVKTGTITIVSYFPNPGNILRPGLFAKVRTPIETRTGGLLIQQ
                     RAVKELQGTYEVAVIGEENKVAIRKVTLGPRIGSLWLIDEGLHPGEMVIVEGLLKIRD
                     GMVVTPKATSDEPQPGSESSPDPALAPHN"
     gene            20083..>21132
                     /gene="pgi"
                     /locus_tag="VLA60_06340"
     CDS             20083..>21132
                     /gene="pgi"
                     /locus_tag="VLA60_06340"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSF09014.1"
                     /translation="MTSLTQRPVWKSLEKHYQLIKDFHLRTLFGDDPVRGQRMTMEAE
                     GIYLDYSKNLITGETLRLLCALAESVDLRARIQAMFRGDKINITEQRAVLHTALRAHR
                     NTSIMVDGRDVVPDVHSVLDQMANFSTRIRSGEWKGYSGKPIRNIINIGIGGSDLGPV
                     MAYEALKHYSDRYLTVRFVSNIDGTDLAEATRDLDPAETLFVVCSKTFTTIETLTNAQ
                     SARDWCLKALHDQQAIARHFVAVSTNRNEVQKFGIDPANMFGFWDWVGGRYSLDSAVG
                     LSLMIAIGPDHFRDMLAGFRAMDEHFQSAPFENNLPVLLGLLGVWYVNFFHAETHAIL
                     PYDQYLCRLSAYCQQL"
BASE COUNT         4850 a         5293 c         5823 g         5166 t
ORIGIN      
        1 ggtcaggata attcttggat gtggcatgtt gtgtctggca ttagtcaccc cgatttcggg
       61 ttgggcacag aatgcttctg aagaagtgtt cctggtggat aaagctcgtt ttaccctgga
      121 gaattttctc gcggatcccg acatgcagtg gtttcgggat tatatgaagg acgcgcgggg
      181 cattttaatt gttccccaat ttattaaagg ggcctttttt gtcggggggt cggggggaag
      241 cggtgtgttg atcgctcgtg atgaaaaaac caacgaatgg agctatcctg ccttttttac
      301 catgggcggt gccagcttcg gattccaatt cggcggacaa gcttcggaag tcgtgttgtt
      361 agtcatgacg caaaagggga tcgattccct gatgagtacg accttgaaat tgggcgccga
      421 tgcttccgtc gcggttggac caatcgggag aggggttgag ggatcgacgg cgcccaattt
      481 gagtgcggat ctcttatcct tttccaaggc aaagggattg tttgcaggca tttcactcga
      541 aggggccgtt gtcgccgcac gggatgcgtt aaataccagt tattataaaa caccaaccaa
      601 aacgatcgat atattggtgc ggcgtagtgt gaataatccc caagccaatc ctttgctcga
      661 agtggtcaag aaggcgacca atggaaacaa ttgaggtttt tttcaggaaa agctgacttg
      721 gctagaaata caaccatttg gcaggctagc gtctgaaagc gtgatcgaca tgcttcgccg
      781 atttttcctc tgcatccctg atccattttt tctttagaca tatagacaga ggaatgaaag
      841 gaattcatga atgggtgaca taaaagcctc cgattgccgc agcgaaaagc cgggattcag
      901 gcaggttgtc cggcttaaga gactcggatt ctcaagcgtg ctcgcgatcg tactggttgt
      961 tgcagggtgc gcgcagaccg aacaggctgg aaacgtgcag acttcaggat ttatcagaga
     1021 ttattccctt cttcaggagg gaggagaggg ggaatcgctt ctcgtgtaca agaatcctca
     1081 agctgatttt tccacttatc gcacgttata cgtggacccg gttaccgtct tattgtcaaa
     1141 ggaatcgtcg gttccccaag aacaattaca caaactgggt ggagatctcc gttcaaagat
     1201 gatctggaaa ctcaaagagg attttcttgt cgttcctaaa ttagtacccg gagcattacg
     1261 attagagttg gccttgaccg aagccgtgcc agccgatgtc ggcatggata ttatcaccac
     1321 tcttgtccca ccggctggtg ttatatcggc agccacggga cttgccacgg gcacgcgggc
     1381 ttttgtggga cgggccacag ttgaaggaaa attaaccgat ggagacaccg gcaccctgct
     1441 ggcggcagcc gtcgatcgtc gaatgggagg tcgaacgctg gatggctcaa tggattcatg
     1501 ggatgatgtt cagcaggcct ttgagtactg ggcaaccagg ttgtcccagc ggcttcgtga
     1561 ttggcggaca acgaaagagg cccttccccg gtcaccttga atgaaggcac ggttgagtcc
     1621 ttttatgaat gggattgcct tgattatcct ccgcacactc tcccgtggtg ccctgtctag
     1681 ggccgatccc taaaggacac ccttccgtcc ctgataggca tttgaagagg acctgactca
     1741 actggaaaat ctaaagcact gattttctct gaattttttt ggacacgtcc ttacatatgg
     1801 atatatgtcg gcccagttga tgcgctgtgg tcgataaaac tttgtttccg gtgacgggtt
     1861 caacgggacc acagagtaat atcgtaaaga agcttccacg tatattgcgg actccgccgt
     1921 caatggcgat gacgtaaagg gaccatgacc atgggtgccg ccgtacaact ccacaaccgt
     1981 gagcgcgccg cgctattacc cattcgctca cttgcaaacc aggctttctc acgcgccgcc
     2041 ggcgcacccc tgatcgaagg caacaccatc cgactgctcc aagacgcgtg cgagaattat
     2101 ccggcctggc tcgatgccat ccgcggggcg aaacagtaca ttcacttcga aaactacatc
     2161 attcacgaag acgatgcggg tcgaatgttt gccgacgtgc tcatcagcaa agcgcaggag
     2221 ggggtgcacg tgcgtctcat ttacgactgg atgggcggat tcggtagagc gtcgcgcgct
     2281 ttttggaacc gtttgcgcgc gggcggggtg gaagtgcgtt gctataaccc gccgcgctgg
     2341 gactcccctt ttggctggct cagtcgtgac catcgtaaga cgatctcggt agatggcgag
     2401 gtgggttttg ttaccggctt gtgcgtgggg cgaatgtgga tcggtgtgcc tgaaaagaag
     2461 gttgaaccgt ggcgcgatac cggcgtggaa gtgcgcggac cgtcggtgac tgtcattgag
     2521 caggcgttcg cagatgtctg ggcgatgatg ggggagccga ttcctgagcg cgacctggtc
     2581 aaaccagacg tgcacgcgca gaaaggcaaa acggcccttc gcgttgtggc gagcgtgccg
     2641 gcgacggcgg ggatgtttcg cgtcgatcta ctgatggcgg cccttgtgag gaaacgcctc
     2701 tggttggtcg atgcgtatta cgccgggacg acctcctatg tgcaagcgtt gcgtgcggca
     2761 gcgagcgacg gcgtggacgt gcgcctgctt gtgccgaacg ccaccgacat tccgcttgtt
     2821 aggctcttgt cccgagcggg gtatcgtcct ttgttggaag cgggtgtgcg aatcttcgag
     2881 tggaacggga cgatgctgca cgcgaagacg gccgtcgccg atggacattg ggcgcgcgtc
     2941 ggttcgacga atctcaacct cgccagttgg ttaggcaatt gcgagatgga tgtcgtcgtt
     3001 gaagacgaac ctttcgcgtc cctcatggag cagacgtatt tgcaggactt ggagaacgcg
     3061 accgaggtgg tcttagatgc gaagcggaag gtgcgcgcgc ccaatcagcc gcgccatccg
     3121 catcccataa tctccagcgt cggcgggagc ggcgggcgtg ccgctgcagg cgccctacgc
     3181 ctcgccaaca ccgtgggcgc ggtgttcacg aaccggcgcg tgctcgaacc cctcgaagcg
     3241 cgtatcacgg tgacggcggg agtgatgctt ctgacgttga cgattctttt tgcgtttttc
     3301 ccgcaactgt tagcctttcc actcgtcctc gtcttcacct ggattgccgg cgcgctactc
     3361 tacagaggct acaagctgta ccgcggcaaa gctcagtgag agagtggaga ggtccagcaa
     3421 ggtggatcgg cgtcgatccg ttcagaagag acgtccaagc gggcgcgccg aagagaagtg
     3481 tgattcggct agagacggag agggctgtag cggttgacgg tcacgagact gttgacccat
     3541 agcaccactg aagtctagac gcgattggca ttacttgtgg atagcgggac aatcaaggtt
     3601 catgttggca aggtatttct cttggacatg atcctgctga gccacggaag aacgccgatg
     3661 cggtggacga agacgagctg cgcgggagaa aatcggcatg gatggtcaat acggtgacgg
     3721 gataggggtg agcgtccccg ggatccaaca atctcaaggg atagacaggt tgacttcgag
     3781 cgccctgtct tgtcggtcac aaggtcgata cgtcttttcg ctcgacctag cggcggttct
     3841 cgagccctgt cgcctttagc aggtcgaacc ggcgccggtg gtgatcatca aaatgtgcac
     3901 ggattacagg aggactcatg ttcggaacca ttcgcaacat cgaaggtgaa cagatcgact
     3961 acaccttcca tccgggcgat aaaagtcgga aggtcatcgt ggtcctcggg cacggggtca
     4021 cgggcaataa ggatcgcccg ttgctcgtcg cgctggcaga agccctgtca cacgacggaa
     4081 ttccgacact caggctgtcg ttttccggca acggtgattc ggaaggtcgt ttcgaggact
     4141 ccacgatctc caaggaagta gaagacctgc gctgcgtgct cgacgcgctc gacggatgga
     4201 agttcctgta tgtcggccac agtatgggtg gagccgtcgg cgttctgctg gccggcaaag
     4261 acacacgtct tgtcggactt gtttcactcg ccggcatggt ccatacggaa gcgttcgcgc
     4321 agcgcgagtt cggcgaggtc acaccgggtg tgggcttcat gtgggacgag ccggcgtgcc
     4381 cgctgtcgca gactttcatc gacgacaccg cggcaatcgg caccgtcgtc gcccgtgctc
     4441 cgtcgatccg ggtcccctgg ctgctcgttc acggaactga ggatgatgtt gttccccttc
     4501 aggactcgct cgacattttc gaaagagcca ataaacccaa gcagctcgtc cggattgaag
     4561 gtgcagacca tgtgttctcc gagcacatac cggacatgat caaggtcgta cacgaatggg
     4621 ttcgagagca gatcggacgc gcgtaagtcg atcttgtatt tgataaagga gtgataaaac
     4681 gtggagtcgt ggctatgtcg tcacggccat cggatgtgtg attaattaag ccccagcctg
     4741 acaaagttct caggagagga tacgacgaaa tggcagtcca aggtcgaaag ctgttctagg
     4801 gctttgtgcc ggtccggttt ttttcggaaa gatacatgcc gacaagccat gccaccaaga
     4861 cggcaacata aaattgtccg atgatcgcct cgagatagga gagggtgcgg gcgatcggcc
     4921 taaggggcgt cacgtcgccg tagcctaagg tggtaatggt gacatagctg tagtacagca
     4981 attcggaaaa ctggactcct atggcggtgg aagtcaggcc gtggaaggag tgcgggttga
     5041 tgatgttgat aataagataa aacaccgacc agttgagtcc gatcaggaga taaatgcaga
     5101 tggcaccaat aattttattc agggtgattg ggcctgaaag gagcaaatgt cgggaagcaa
     5161 tccaggtgct gaccagggag aacacaagga gtatggccat gttgacaaac tgaagctcag
     5221 gaatgtctaa gaaatagtcg atggtggcag tcgcgactcc acttaccgta aggattcctc
     5281 ctcctaccat caaccatttt ttttcactga catggctcca tattcccatc aggagcatga
     5341 ggcaaagagc gggctcaagg aacaaacgcc gggtctcact tgtgatccct acttctcgtg
     5401 cgatggccac cgagagcagg aggaacaaca gacctcctaa caaataccca aagttcatcc
     5461 tcttgatagg catcggcgtc ttctccgaat ggcgaaagag ggaggcctca tttatatggc
     5521 cctatctgtt tcttgtggcg ctttaatggt cggtagggga agaatagcgc accttcagtt
     5581 ctgatttttt gacatagagg gtcttccgca aaggcgtctt tccagtgtgg caggaaaggc
     5641 cgcaaaatcg acgtgattgg tccaagggca acagtccaga attccgaggg gggtggtagt
     5701 ctcaagagtc cttcatccga tacatagaga tttgcgggat tggcatctgt gaaaaagact
     5761 ttctcccgcc aattgcggcg cggggtaaat gaatgccgac ggtgagcaca aacccttctt
     5821 ccggttccag gaatttttca gagtctatca taggaaaaag ggtagggtcc ctggatttaa
     5881 gatgcaaaac tcaaaggtat tctccataat acacctcgtg aagataaagg ggagaggatt
     5941 caagagggtc cactaagagg gcggcgtttg gatggacatt tctccctggt gctccatgcc
     6001 cgtatgaaaa cggttgtgtt ggtcggtcga attttttgag aagggttgat ccccggtccg
     6061 aatccgttga gagagccgca tggccttggc cggaaaggat tgatgagatt tttatatttg
     6121 tgggaggcat ttcatgacga tacgtggaaa aattgatctt aagaaaaggc cagggtatgg
     6181 cctacaacga acatggtcct tgattctcac gattctcctc gcggcatgtg cgcaaaaccc
     6241 tgcctatctc tatgagatgg gtgagaggcc gatacccata ggaatcgaaa aggaaatcat
     6301 cccgcagacc accactcagg atgaggtgtt aaccctgctt ggagagcccg tcgcccgcct
     6361 taaaacccag acgcatgagg gacatattca catctggacc tattcgtatc tggatcttca
     6421 gagcacctca caggacaagg gggaatcgct gaccatcacc tttgatgata aaacccttgt
     6481 ggtccaaagt gtcaccagag gcccgcttta acacaggatt ggacaatcag gaagaacgca
     6541 tttatgcctg ttttgatagg ttcatgggct tccgaagaat ttttctgtgg tgtacttcta
     6601 gcctggcttc tgaatgctga atctaccttg aacatgtaac atggctcgga aagttttcaa
     6661 acggattatc gaggagctat ggttttaggc atggttcacc ttgcggggtg agggactgtc
     6721 cgttctttct ctctaacagg gtgttgaaaa agtccgtcag cggcgttctt gcctctaggc
     6781 cgtgttcccg tacgggcttc tccttttcaa aaatcagggt tcatcgccga cccttcgttg
     6841 gtaagggcga tatgaggaca tcggggttct cgagcgatcc ctcccatggc cggaaccgta
     6901 ttttggtgaa aaggcctggg ggtatttttg gcttatcctg gttaatccta cattgcatga
     6961 aatggaaggg ttacccaagg ccgcaatcag cctttaacgg tccgattcgt gccgaatgtc
     7021 ttatgtttgt tggccgttgc tggtgaaaaa ttttctcaaa tgtgttaaaa gcatccggga
     7081 ggaggtggaa gatttcatcg agaacacacc gtggagatga tgtgtttcca gccagatcga
     7141 acgggaatga catggccgct ctccatacct taattgcctg ccatgaatgc gatctccttc
     7201 atcgtattca cccgctacat tatggtgagc gggccaggtg ttcgagatgt ggcgcatcgt
     7261 tgtatgcgaa aaaacgggat agtttcgaac ataccacaat actggccttg gccagtctca
     7321 ttttctttac cctggcgaat gttttcccgt ttatgacatt cgagctcaat ggccgtgttc
     7381 aggagagcct tctatccaca ggggtgaggg agttctttaa acgggaaatg tgggcgttag
     7441 gggtcctggt gttgtgtgcg agtatcattt tcccagccct gaaaattctt ggcatgttgt
     7501 acgttcttgg gccgctggaa ttcaacaaac gaccgtggca ctccgctctg gtctttcgga
     7561 tggttgagca ctgccggacc tgggccatga tggacgtgta tttgctggga gtgattgttg
     7621 ccgtggtgaa gttgtcggat ttggccaatc tggttcccgg tgtggcaatt tattcattcg
     7681 tcgccttaat cctgaccttg gctgccgccg actctgcact ggacacccat gcggtctggg
     7741 aaaaaatgga gaagatttca tgagggcctc ttaccccacc gcggcccggt cggggcttat
     7801 gagttgtcat gcctgccatc aactcagtcg aatacccccg atggtgaatg aggtggatgc
     7861 catctgtcct cgatgtggag cccatctcca tctgagaaaa cccaatagta tttcgcgcac
     7921 gtgggcactg ttgattgccg cctacatttt gtatatcccg gccaatctgc ttcccgtgat
     7981 gacggtgatt tcgtttggga aaggcgaggc ggatacgatt ctgagcgggg tcaaggaact
     8041 cattcatgcc ggaatgttgc cgattgcctt gctggtgttt ttcgccagca tcaccgtgcc
     8101 ggttttaaag ctcctggcgc tgacctatct gttgctttct gtccaataca aatctcaatg
     8161 gcgcccaaga caacggacca agttataccg gatgaccgag gtggtcggcc ggtggtccat
     8221 gattgatatt tttatgattt caattttaat tgccctggta aagcttgagg ccgtggcgac
     8281 aattgaaccc gggcctgggg cgatctcatt cgccgccgtt gttattctga ccatgtttgc
     8341 cgccatgagc ttcgatcctc gattgatctg ggacaatata aaggagaaat catgaaggac
     8401 aaggaatcca atcctgacga tttcgaggaa tttccggaag tcacggtaca aaaaaagcgc
     8461 gggatttcca tcgtgtggat tattcccatc gttgcggctc ttattggagg gtggctgact
     8521 tacaaaacga tctccgaaaa ggggcccacg gtcaccatca cctttaaaga tggggccggg
     8581 ttggaagccg gaaaaaccaa aattaaatac aagtccattg aggtcggaat cgtcaaaacc
     8641 gtacatatca gccatgacct ctttcacgtg gttgtgaccg cagaactcag caaacaggct
     8701 gagccccacc tcacggaaaa tgcccagttc tgggtggtcc gtccccggtt gggattgggg
     8761 ggtgtttcgg ggcttgagac attagtgtcc ggagcctata ttgccatgga tcccagacca
     8821 ggagcttcag ctcgaacttt cacaggattg gagaaaccac ctggagtgac acgagaagac
     8881 gaaggggccc aattccaatt gcaggcggaa aatctgggat cgagttcccc cggtgccccg
     8941 gtcttttatc acgatattca ggttggacgg gttctagatt atgtcttgga caaggaaggc
     9001 gagggtgtgt tgatcgatat tttcattcaa gctccgcatc atctgcgggt acgggatacc
     9061 agccgattct ggcagttaag cgggtttgag gtctcccttg gggcagaagg tctggatgtg
     9121 aaaatggaat cgctctcctc cctcctcacg ggtggaatcg cgttcgataa tccggccaat
     9181 gcaggcgggt cgggtgaacc cagccaaccc ggcacggcct ttaaactttt caaaaatttt
     9241 tccagtattc aagatgagaa atatgtcctg acgcgtccat ttgtgatcaa ttttgatgga
     9301 tcagtgaggg gcttgagcgt cggggctcct gtggaattcc gtggaattaa aatcggggtc
     9361 gtctcggata tcgcggtcaa tctgaacccc aagacgcttg agattaaaat tcccgtgctt
     9421 atcgagatcc agccggaacg aatcaccgcc ccacagctcg ttaaagcaca ggtgttagaa
     9481 gataaatacg cgatcatgaa gcatatggtg aaacgtggct tgagagctca actggagacc
     9541 gggagcctac tgaccggaca attgtttgtc gggctcgact tccaccagaa tcttcccgag
     9601 aaagagctga tcatgaccgg caaatatccg gagattcccg ccatacctgc agcgatggat
     9661 gaattgcggc gaacggtcac cgatgtgatg gcagagattc gacgcttgcc tcttgataaa
     9721 attgccaggg aaattctgga aaccgtcgaa ggaggcaacc gtctggtcaa ttctcctgac
     9781 acgcaaaaag ctgtccacaa tcttaatgcg gcattaggaa atgtggaaaa gctcactgag
     9841 ggtcttgacc ggcaggttga taccctgatg acgaatttgg acaacaccct ggtgatggtc
     9901 cagaaaggaa tacggcaggt tgatcccaat tcacctgctg cggtgaacat gaacaatgcc
     9961 ctgaaggagc tgtctgctgc ctcccgctct attcgggttt tggccgatta tcttgaacaa
    10021 catcctgagg cactagtcaa agggaaacat tgaatgaggt ggacatgaag agcatacatg
    10081 gtttccgtat catcgcggcc tgcgtaattc ttctgactgt gcttgggtgc gggacgagtc
    10141 aaccttcgca tttttatttg ttgcgagccc tgtcgccatc ctccgtccca ggcctgtcag
    10201 acacgaaagc ctcaggcctg tcctttgggt tggggccggt cacccttcca aaatatttgg
    10261 accggccgca aattgtgact caatccggtg ggcatgaggt cgagttggca gaatttcata
    10321 aatgggctga accgttgagc gacaatgtgt ctcacgtcct ggcagaaaat ctctcagtca
    10381 tgctttcgac ggatcgaatc gaacagtatc cgtggaggag aacaactccc gtggatttcc
    10441 aaattgtcgt ggatgtcttg cagttcgatg gtaccagggg aggggaggcc gtcttgtcgg
    10501 cacggtggag ccttttgggt ggggatgaac aaacagtctt caccacaagg aaaacgcatg
    10561 tgacccatca tccgacaagc caggattatg aagcgctggt cgaggccatg agtcacaacc
    10621 ttgaggatct cagtcgcgag atagccgagg cgattaaggt gcttctcccc acaatcctac
    10681 ctggctcaat ttcataagct gccatgggca aggttagttt ttggctgggt ccagagacac
    10741 accgaagcat gtccgtggtg gtcatggact gccaaaaaca cacaactcaa tggcacgtgg
    10801 cagccggtat gttttccgat ggtcatccgg atgatggtgg gaatccgatt caacgtgctg
    10861 aacatgacct acacttacct ggagaacaat tcatggggat gctttcgacg gtcaaacatg
    10921 aacttaaaga agtcggttta gtgaccctgt atttcttctt ctgtttcggt gtcatcctga
    10981 ccctgaagaa gctgatgttg gaaggctatc atatcgagtt ctatgccttc tcaacggcac
    11041 tggtcggcgc acttatcgtt gcaaaggttg tgggcctgtt gggaaaaacc cgagcgggca
    11101 ccaggtttga tgcccggtat tcccttggga tggctactct gtaccggacc atagtgttta
    11161 gtggatgcac tttcctggtt ttatttgctg aaaaaatgtt tcatgcgttt cgggagagtg
    11221 atctgttggg tgcggctttc atggacgtgt gggcacataa ggatctttct ctcattttcg
    11281 ctaaggtgat gtgcatcggg ttggccttta tgggattttt tctttatcga ggaattgatc
    11341 ggcggttagg agaggggacc ttgagacgca tggttttcga tcccccaaat taaaagcctc
    11401 ctttaatgtt ttaattcaag taatgtgctc cggaatatcc acattgggct cttcagatta
    11461 cccggtgggc ttgctccgga aatttcaatt tcttttaaag gtgggcccat tcagctgaga
    11521 tggttaagat tcgtcttttg ggtttccttt cattatgcat aggacttggt taggaataga
    11581 gagtttccag ataaagtatt tagatgtgaa ggataccacc ttggtcgatc ccatcagtga
    11641 tcctgcatgg atttctgtct ggacagatcg acgtggatcc agaactggta tttcgtccgt
    11701 gtaaaaagag attcccatca cacggttaat aatctacggc aagcgcgtca atgcgcatat
    11761 gccgatagaa gtgccgatga tcacgcagct cgccccgata catcgccgat ctggtatacg
    11821 ccatcacgac aaaatacatc tgttccactt cctgaacacg ttttattgca aaccatgtcc
    11881 agggagaatt accgaaagct ctttcattga gaggagcacg gggatgctac ggggtgaatt
    11941 cacctcgtca gtaccgctct agaatggtgg gtagaaatca cgttgcggta tgatcgtgtc
    12001 tatgcccact gccaaggaag gaaagcccga tgaattacgc ctcgcttgca gaagaacagc
    12061 actattaatt ttctgcactc atgaaagccg gtcatcggga aatcaaaatg gctgcggtgt
    12121 cggggggatc ataagctcac gattagccgt gagatttggc aaaaccgagg cttgtgaggc
    12181 tatcgtcctt ggctagccac cagttggcgg ttccccgtcg ccagcccgcg gcgtcatgcc
    12241 attttaccac ggagctttag agcctgatcg ggtatctgtt gcaggaggac tggagtcccg
    12301 agcagaacgg tctgtggttg gcgatcaaca ggctatcacc cagaaggatc ttgaccacat
    12361 catgcacaaa ctcaaccacg gactcaggaa atgccctggc agaaaaacac ccaatcagtt
    12421 gagtctgcga gggatatttt gagtgtgttt tggcatcaat ccatttgttg cattagtgaa
    12481 cggaataatc aaaaaaagcg attctttatt atttcagcgc ttcagccccg cccacgatat
    12541 ccatcagttc cttggtaatg gcctcctgac ggctccggtt atatttgagg ctgaggtgct
    12601 tgatgagttc acccgcattg cgggttgcgc catccatggc agccattctg gcggcttgtt
    12661 ccgcggccga tgattccaat aaaatccgga aaacctggcc ctgtgcatac ttgggaagaa
    12721 gggtcgagag taactcctcc tgatcgggtt catacagata tccacctccg gtttgcgtct
    12781 cggccgctcc aaattctttc agcggttcaa taggaagaac tttttccacc accacttgtt
    12841 gttgaacgac gtttttaaac tcgttataga cgatatatcc ctcatcaaag acaccatcgg
    12901 caaatgcttc taccgcggcc aatccgactt cgagagcatg ctcataggtt aatcgttcaa
    12961 acacattcac atttggatcg cgaataggcc aatgacggcg gcggaagtac tcgttccctt
    13021 tccggcctat cactccaact attacctttg ctccggtttc ttcacatttc tgaatcacct
    13081 ggagggcttt gcgaatcacg ttcatattga acgctccgca caatccacgg tcgcttgtta
    13141 ccaccgtcac ttgaataatc ttgaccgggc gcttttgaaa caaggggtgg actgactgat
    13201 tgacccgact gctcaagttg gcaagaacac gacgtaattc ttgtccatat gggcgaaacg
    13261 ccagaattcg ttgctgtgca cggcggagtt tggatgcagc caccatcttc atggcctttg
    13321 taatcttttg tgtgttcttg accgagccga ttttacggcg taaggcctga agatttgcca
    13381 tctcccatcc tcccatattc tgaatttgat ctgttgaaca aacccgttac ctgcttcctg
    13441 cacgacatga ggaaattctg gggaaggcca gattgtttac gaactcggat tttgaccatc
    13501 tcccattttt caaatgagga atgctatagc aaaccccgaa gtacagtagg ttcaccatct
    13561 gtttttaaca gaattggtca tgagtgagag aaagtattgt gcatcaaatg gccggaggtt
    13621 gcaagagaag taatgggaaa ctcaagtagg ctacttcact tggatagggg agccgaccag
    13681 cactgcattc catcgttccc ggaacatcaa caaccctggt cttacatcac cacacacaac
    13741 cgcatgctca gattttttgt tcaggcttca tttcctgatc catgggacca ggttctaatg
    13801 gggaggaaac aggttgcccc ttttttgcag ggggagacca accgccaccc agggctttgt
    13861 atagccggac gatggaacta agattttgct gtctggtgga gaccaggctc agctcggcct
    13921 gaaagagatc ccgttgggct gttaagacat ccaggtagga cgcgaaccct ccccgatacc
    13981 gtttatccgc aaaatttaac gcggacttga gggatgctac ctgagactcc tgtgcttcag
    14041 tttgctcgtg ggtcttttcg atcgtaatca aggagtcctc aacctccctg aaggcattga
    14101 gaatggccaa ctcgtactgg gccagagctt gttgctcttt tgccgtctgg accccgacac
    14161 catagccaag tgccgtggcg ttgagaagcg gaccggttag ggtgcctgat gctccagcag
    14221 tggcaaacgg ccccgtcccc atcgagttta actgaaagcc cgctacccct ccgccggcat
    14281 tgatactgaa ttgtggaaaa cgttgagcct gggccacgcc gatgttggcg gttgccgcaa
    14341 cgagcttttg ttcggcttcc agaatatccg ggcgttgact caggagactg gaaggcaacc
    14401 cgatcggcac tttaggcggc aaaggttgtt ccgtgaggac caacccccgg gggattgcat
    14461 ttggacggtg tcccaacaag acgctgatcc ggttttcagc ctgcaccacc tgctgctcca
    14521 gattggccaa ttgggccctt gtgccggctt gttcggcttc gaagcgatcc agatccagct
    14581 tggaagcata cccgtgttca tattgtgacc ggctcaatct cacggcctca ttccagattt
    14641 tgagcgtgcg ttgggcaatc tgtacctgga gatccagagc ccggagtgtg aaataggatt
    14701 gcgctacgtc gctgaccagt gcgatgacca ccccacgctg gttttccacc cttgccagta
    14761 attcagcctg tgtggcttcg acattgcggc gaatccgtcc ccagagatcg atttcccatt
    14821 tgatgccgcc cacgaaggat tgtctcgaaa agtccaggtc accccgtcca ctttgtccgc
    14881 cagcttgccc gggtataacg gccccgtttc cggtggacag cccattattc gtattgtgat
    14941 agtacatccc ctctccttga tacccgaggg atggggcaaa atcgaatttg gcaatgacaa
    15001 gttggttgtt aaactcctcg acagctgcgg cggccactcg aaggtcaaga ttttcttcta
    15061 aggccgtacg gatcagtttt tgaagttctt catccttgaa caattcccac cagggcatat
    15121 tcgcgataga ttcggaggtc ccaggtgcaa ggcgccagga atcggatgtg ggaacgtcgg
    15181 ggcgtttgta atccggtccc atggtgcaac cccaggcgat cagaagactc aacaggatag
    15241 ggacgcaact tctatgcata ttaatgacct ccctggagtt tgaattgagt ctgggcatgt
    15301 tttgtttgtg gggaaacaaa cgattcttca tcttccagta gctcggaatc ctcaaactct
    15361 tggccgtcgg gatcttggtc agggggatcc tcatgtcgat ggaccaaccg ctccactacg
    15421 taaaacgaga cagggatcag gaaaatggcg ataaagctgg cggcaatcat tccaccgatc
    15481 acggtgacac ccagaagaat tctcccatgc gccccggacc ccgaagagag aacaagtgga
    15541 accactccga agataaaggc aaaggatgtc atgaggatcg gtcgcagtcg caggcgggct
    15601 ccggtcagag ccgcatcgat gagagaacga cccttttcgt actccatttt ggcgaattcc
    15661 acgatcagga tcgcgttttt cgcggacagc ccgatgagca tgacgagacc gatctgggtg
    15721 aacacatcgt tttccattcc gagtgcccac aaggcaccaa aggcaccgac gactgcaatc
    15781 ggtgtacaga tgagaacact gaacggaagc ccccagctct catactgggc cgcgaggatg
    15841 agaaagacaa agagaaggga gaagccgaag atcacgctgg ccgggactcc ttcggaggca
    15901 actttctctt gaaacgacat tccgatgaag tcgtacccca tctcgcgcgg catagtttcg
    15961 gtgaagactt cttccatggc ctgcatgacc tgaccggagg taaagccgga gttcgctgaa
    16021 gcgaaaatct gcgcggatcg gtagccgttg aatcgcatgg tgaattccgg accagaaatt
    16081 ttttccatcg tcaccagtgt cgagagaggg accatctcat cggatttatt ccgcacgaaa
    16141 aattgtccca cgttttcggc cttggtccgg aattcccctt cagcctgcac atacacctgc
    16201 cagacacgcc cgaaccggtt gaaataattt accatcacac cgcccataaa ggtctgcagg
    16261 gtcttataga catcttcaag tttcacgcct tgcttcagca ctttgtcgcg atcgacatgg
    16321 gcaaagacct gtgggacggc ggggatgaag gtggtgttca tggacgcgat ttcggggcgc
    16381 tgtcgtgcgg cttccatgaa cgtgttgagg ttgtcggcca agaaagccac atcctgtccg
    16441 gaacggtctt ccagaataaa ggaaacgcca cccgatgtgc cgacgccggg aatggcggga
    16501 ggcggaaaaa gaaaggcgtt cgcttcgggt aaggctgcga gtttctcgtt cacatttttg
    16561 aaaatagcct gaagtttttc ctcgggtttg gtccgttcct cccatggagc gagagtgacg
    16621 aaaaagaacc cgttataggt cgtattgacg gtgctcagga gactaaaccc tacgatggtg
    16681 ctcacatgtt gaattccggg agtttctgaa agaatggctt cgattttttt tgcaacctca
    16741 tccgttcgtt gcagggaggc ggccaacggc agttggacgt ttaaatagac atatccctga
    16801 tcctccatcg gaagaaaccc agtgggaagt ttggctccca acagaccggc acataccgca
    16861 gccacgacca aaaagagcat cgcccggccc gactttcgga tcaggtggtg gcaatacccg
    16921 acatacccgt ccgtggcccg cccgaaccaa tggttgaacc acccgaaaaa tgcccccaac
    16981 gggcctctgg ctggagttct gggcctcaga atcatcccgg aaagcgcggg gctgagggtc
    17041 aaggcattga aagcggagat gatcaccgat aaggcaatcg tgagcgcgaa ttgttggtaa
    17101 agtcgccccg taatgcccgg aatgaacgcg gtgggcacga agaccgccgc gagaatcagt
    17161 gcgatcgcga tcaccggtcc ggagacttcc tccatggcct ttctggtcgc atccttgggc
    17221 gaaagacctt tctcgatatg atgttcgacg gcttcgacca ccacgatggc atcgtctact
    17281 acgagcccga tggctaatac cagtccgaat aacgagagag tgttgatcga gaaatccaac
    17341 aatgggaaga tcgcgaacgt cccaaccagc gacacaggca cggccagcag aggaatgagc
    17401 gtggcccgcc atccctgtaa aaagaggaag acgacgaata tcacgagcac aaccgcttca
    17461 aagagagtat gcacgatctc cttgattccc tccctgacag ccagtgtcgt atcgagtgag
    17521 atggcgtaat caaggtcttc gggaaaccgc tctttcatct cctccattag cttctttgcc
    17581 cgatcgaccg tatcgattgc gtttgacccc gggagttggt agacggcaat cagcgcgctg
    17641 ggctttccgt tgaaacggcc tatcatactg taggattgcg cacctaattc cacgcgggcc
    17701 acgtccttca gtcggatgat ggacccgtcg ggatcggccc gcacgatgat ctgtccgaat
    17761 tcttcctcgt taagcagacg accttgtgat cggaccgtat acgtgaattc ctggccttgg
    17821 ggaaccggtt gagctccgat ctgaccggca gggttgacgg tattctgctt cttgatggca
    17881 tccaggatct cggtcacggt gatgcccagt ttggcgagat gatcgggttc cacccagaag
    17941 cgcatggcat actggccagc cccaaagatc gtgacctgac cgaccccttg gctccgcgaa
    18001 agaggatcgt tgatgttcac gtaggcatag ttagccagga agatttcgtc ataggtgcca
    18061 ttcggggaat acaggctgaa gagcatcaga ggactggtgg tggatttttt aatggtcacc
    18121 ccgtagttct tcacgtcgga gggcaattga gattcggcct gcagataccg catctgggtc
    18181 aggatctggt catcgttggg gaccgtgttc acgtcaaagt ccacgcgcaa cgtcatctgc
    18241 ccattactgg cgttcgtgga gtacatgtag atcgagttgt caacaccggc catctgctgt
    18301 tcgatggggg tggcgaccga ttgttcgacg gtgaccgcgt ccgccccgac gtaggtggcc
    18361 tgcagttgga tttccggagg cgcaatgtcg ggaaacagcg cgatgggaag ttgcaccatg
    18421 gcgatcaggc cgaggatcgt catgatgatc gaaatgacga tagcgacgat gggccggtcg
    18481 atgaaaaatt tggccattag gcagctttta attatgtggc gctagcgccg ggtctgggga
    18541 actctcagag ccgggttgtg gttcgtccga agttgctttt ggtgtgacaa ccataccgtc
    18601 tcgaatctta agaagtccct ccacgatgac catctcgcca gggtgaaggc cctcgtcgat
    18661 cagccagaga gatccaattc ttggccccag ggtcactttt cgaatcgcca ccttgttttc
    18721 ctctccgatc acggcgacct cataggtacc ctggagctcc ttgactgccc gctgttggat
    18781 taacagtccc ccggttcgag tttcgatggg ggtgcggacc ttggcaaaaa ggccggggcg
    18841 caagatgttg ccggggttgg ggaaataact gacgatggtg atcgtcccgg ttttgacatc
    18901 cacctgacgg tccgccagga cggctcttcc cttatgtggg tatatgctcc catcagccag
    18961 aaccagctca agtctttttt catgggcggg gtttaatttt tcctgacccc cagccttttc
    19021 aatccgttcg gctagtttta aatattcccg ctcgctaatg ggaaagttga ctttgatggg
    19081 gtccaactgg gatacggtcg tcatcaacgt attctcgacg atgagatcgc cgatttgtgc
    19141 cgtggcgatt ccggctatcc cattgatcgg ggaggtcacg ttcgtccagt taagattcag
    19201 ttgagcattc tccagtttgg cacgcgccga agcgaccgag gccaggttgg ctgcgcttgc
    19261 ctgtatggca tcatccagtt ccttctggct gatggcgcct tccgctgcca gaggagtgtt
    19321 ccggttaacg tcaagctggc tctttttata ggccgcttcc gtacgggcaa gttccgcctt
    19381 cgcttgatca agggcagctt tgtacggtcg tggatcgatt ttaaacaaaa ggtcattggc
    19441 cttcaccact gacccttccg tgtaatgacg ggatatcaga tacccctgaa ttctggcccg
    19501 aatctgcgca tccacatagc cgacggtggt tcctatccac tccccataaa tcgggacgtc
    19561 cttttgtatc acctctgcca ctaacacttc aggcggcata gccgtggatt ccgtctcttt
    19621 gcggtcacat ccaaagatta tcccaagcag aaggaatacc agcagcagac cgaaccctcg
    19681 cctgaattca tgcagtgggt gtggtagctt ttttacgttg aaagaatttt ggttcgggaa
    19741 gggagcaggt aaacagtgtc ttgtcaattc tcactcccta ctttatatcc ggttgtttcc
    19801 ccatgcgggc caggaggcaa gcgccttatt ctttcatggt tcaggttcaa atctcaagta
    19861 cgcacctgac tgttttttct tcgcggtttt ctgatcctgg atggcggatg tgtatttggc
    19921 agagatggaa acgggagtga tgtgattcat ggcccgtatt cccttcttcc cccatcgggc
    19981 atctcggaca atcgatggtc acccgttgac acttttactc taccttacga ccatagctat
    20041 aatcgtactt ctgcgattaa ccttacaacg gagtctcttc tcatgacctc tctcacgcaa
    20101 cgccctgtct ggaaatccct cgaaaaacat tatcaactga tcaaggattt ccacctgcgc
    20161 accctgtttg gcgatgatcc cgtgagggga caacggatga ccatggaggc tgaggggatc
    20221 tacctggatt attcgaaaaa tctcattacc ggtgagacct tgcgcttact ctgtgctctg
    20281 gcggaaagcg tggatttacg agcacgtatc caggcgatgt tccggggcga caaaattaat
    20341 attactgagc agcgagcggt ccttcatacc gctctgagag cacacagaaa cacatctatt
    20401 atggttgacg gcagagatgt ggtgccggat gttcacagtg tgttggatca aatggcgaac
    20461 ttttccactc gaatcaggag cggggagtgg aaggggtatt cggggaagcc gatccggaac
    20521 atcatcaata tcggtatcgg ggggtcagac cttggcccag tgatggctta tgaagccttg
    20581 aagcattata gcgaccgtta tctgactgtc cggttcgtct cgaatatcga tggaacagat
    20641 cttgccgaag ctaccaggga tctcgaccct gcagagacgc ttttcgttgt ttgttcaaaa
    20701 acctttacca ccatcgaaac cctgacgaat gcgcaatccg ccagagactg gtgcctgaag
    20761 gcccttcatg accaacaggc catagccaga cattttgtgg ccgtttccac aaaccgcaat
    20821 gaggtccaga aattcggaat tgatccggcg aatatgtttg gtttctggga ttgggtcggc
    20881 gggcggtatt ccctggattc ggccgtcggg ctttcgttga tgatcgcgat cgggcctgac
    20941 cattttcggg acatgctcgc cggatttcgt gcgatggacg aacatttcca atcggcgccg
    21001 ttcgagaaca accttccggt gcttcttggc ctcctgggtg tgtggtacgt caactttttt
    21061 catgctgaaa cgcatgccat cctcccctat gatcaatacc tctgccggct cagcgcctat
    21121 tgccagcagc tt
//
LOCUS       DATIAL010000200         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_36326, whole genome shotgun sequence.
ACCESSION   DATIAL010000200 DATIAL010000000
VERSION     DATIAL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36326"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..1640
                     /locus_tag="VLA60_06345"
     CDS             414..1640
                     /locus_tag="VLA60_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF09015.1"
                     /translation="MSQLPQLQRELDSARSRTDRLFSFVCAEAMYERPIPERNRLIFY
                     LGHLEAFDWNQIGRWTLGMPSFHASFDQLFEAGIDPSFGSFPVDQPSDWPTLNEVYRY
                     NARAREEVDRLLASVPAWIVHMAIEHRLMHAETNAYLLHHLDSKHKKPPSDFTRVLSF
                     SLSEETAKDEMIEVSEGIATLGMKPDEGFGWDNEFAQHEVAVPGFLISRYKVTNQQYL
                     RFVQDGGEPSAFWVKRGDAWYVRTMFQEIPLPKSWPVYVTHRQAQAYANWVGMTLPTE
                     AQFHRAAFGTPAGIERSFPWGDEAQMFQHVNVDSQSWDPVPVMAQSTEHSGFGVAQMV
                     GNGWEWTSTLFHPFHGFSPLPTYPGYSARFFDQDHYVVKGGGPHTASRLLRRSFRNWF
                     RQSYPYAHIGFRCVQS"
     gene            1690..3273
                     /locus_tag="VLA60_06350"
     CDS             1690..3273
                     /locus_tag="VLA60_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HSF09016.1"
                     /translation="MRQILQSAGYWGHFLLILILWGNPAYGTDRLVILHSSEHHGVAL
                     PLDQAGETTIGGLARRGTIVEEVRREGGPVLLVDSGDILIGTALSSWFRGEPDILAMN
                     LIRYDAMVAGNHDFDYGIDHLRTLVELADFPILCTNLQSERSLLPCRRSFVTRLGNVV
                     VGVLGIVGKSNFPGTFNRDVAKALSLVNPIDSLRAEALRLRMEGNVDLIIALTHQDSE
                     EDLKILETVEGVDVLIGGHTEGFDGLYAPGLMEPVETMTRPRSVYVKTHRQGRTIGRL
                     DLTISGGSVVQARSYNIPVTVAVPVEPKVQQLLNEYRQQFALHATQVLGEASVRLQGD
                     RPVIRTQEANLGNLLADRMRNTLGTDIALINAGQIRRSLEPGPVTLGDVLSVLPFDSA
                     LVTLHVTGAMLCEVLEHSVSQWPNHSGRFFQISGLQVTYQGKAPVGSRVRSIMVGGVP
                     LDISKTYTVATDAFVADGGDGYDMLTHVTHRTDHQIPLRDLLLNALAEGPLYAEADHR
                     MVFMIGNEEKWRNGVPCLR"
     gene            3504..>4311
                     /locus_tag="VLA60_06355"
     CDS             3504..>4311
                     /locus_tag="VLA60_06355"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HSF09017.1"
                     /translation="MSLPQDARDSRRWWADLLAVWRDTRQIVLTAQIAAIYAAILIPF
                     KAGIPIIPGFVELRPANAIPIVASLLFGPAAAWGAGIGNVIGDCFGTLGPASLFGFLG
                     NFFFGYVPYMLWGHLGWLSSGQAPVIKAWRPMMEYVVVCVIASAVCAGIIGWGVEWLG
                     LLPFVILAPAIFLNNIVMGVFLGPPLLRFLYPRVQRWRLRYEDIRKNNTFQFQPFAGP
                     PRRSDSINHEVIKENLSQAVLECCGLSFQYASGADPVIRNVSFTLRSGEIV"
BASE COUNT          920 a          993 c         1199 g         1199 t
ORIGIN      
        1 cgcccggcgg agcgggtgaa cgggctgtaa gcagggagcc cagagggaac ccgaagagca
       61 gggggcgaca aatgtcgccc ccagctcttt tttttgcgtg ttggcctagt tgaccaggtt
      121 ttgaggagac aggagggtca ctggaaatga gcatccggac ggctaaggtg tcacttttac
      181 cttgaacaaa atagccatta aatagtggaa ccatcatcat ttactttggg gcaagccttg
      241 tcgctattct tgtcaatgac aggaattttc cacgtttgga tgaaattctg actcccgttc
      301 attgaacttt tctagggaga ggcgagttgg tatagttagg gcggtgagtt atagaatctt
      361 gtgaaagtag gttgctgggg cattattagc tggatgttgg ctgaggaggc gcgatgtcgc
      421 aattgccaca attacagaga gaattggatt ccgccaggtc acgcactgat agactatttt
      481 cgtttgtgtg tgccgaggcc atgtatgagc gtcccattcc tgaacggaac cgcttaattt
      541 tttatctggg acatcttgag gcttttgact ggaatcagat tggtcgctgg acactgggca
      601 tgccgtcttt tcatgcatca tttgatcaat tatttgaagc cggtattgat ccttcctttg
      661 gaagctttcc cgttgaccaa ccttccgatt ggccaacgct caatgaggtg taccggtata
      721 atgcgcgggc tcgtgaagaa gtggatcgcc tgttggcatc tgttccagca tggattgttc
      781 atatggcgat tgagcatcgt ctgatgcatg ctgagaccaa cgcctattta ctgcaccatt
      841 tggattcgaa gcacaagaag cccccatccg attttactcg cgtcttatct ttttcactct
      901 cagaagaaac ggccaaggat gagatgattg aagtctcgga aggcattgcg accttgggga
      961 tgaagccgga cgaggggttt ggctgggaca acgaattcgc ccaacatgaa gtggccgtcc
     1021 ctggattttt aatcagccga tataaagtga cgaaccaaca atatttgcga tttgtacagg
     1081 atggtgggga accctcggcc ttttgggtca aacgagggga tgcctggtat gttcgcacaa
     1141 tgtttcaaga gatcccattg ccgaaatcat ggccggtgta tgtgacgcat cggcaggcgc
     1201 aagcatatgc taattgggtc gggatgaccc tcccgaccga ggcgcaattt catcgcgcag
     1261 cctttggcac tccggccggg attgaacgat cgttcccttg gggtgatgag gcgcaaatgt
     1321 ttcaacacgt gaatgttgat tctcaatctt gggatcccgt tccggttatg gcccagtcta
     1381 ctgagcacag tggttttgga gtggctcaga tggtgggaaa cggctgggaa tggacatcca
     1441 ctcttttcca tccttttcac ggtttttctc cccttcccac ctaccccggg tattccgcca
     1501 ggttttttga ccaggatcat tatgtggtga aaggtggtgg tcctcatacc gcaagccggc
     1561 tcttgcgccg gtcttttcga aattggtttc gacaaagtta tccctatgcg catatcgggt
     1621 ttcgttgtgt acaatcctaa tgcatgacgc tgccccccca ccgtgttgcg tatcgaattg
     1681 aactgttcta tgcgacagat cctacaaagc gcagggtatt gggggcattt cctccttatc
     1741 ttgattctct gggggaatcc agcgtacggc actgacaggt tggtgatttt gcattcgagt
     1801 gaacatcacg gggttgcgct tccgcttgat caagccggag agactacaat tggggggttg
     1861 gccaggcgag gcacaattgt cgaagaagtt cggcgagaag gcgggccggt tttgttggtg
     1921 gattccgggg atatccttat aggaacggct ttgtcttcct ggtttcgcgg tgaaccggat
     1981 attctggcca tgaatctgat ccgctatgac gcgatggtgg ctggaaatca tgattttgat
     2041 tatggaatag accatcttcg cacattggtc gagttggccg atttcccaat tttatgcacg
     2101 aatcttcagt ctgagcgatc gctcctgcct tgccgccgct cgtttgtcac tcgcctgggg
     2161 aatgtggtcg tcggggtctt aggtattgtc gggaaaagta attttcctgg cacttttaac
     2221 cgggatgtgg caaaggcatt atcgttggtg aatccgattg actctcttcg agcggaggcc
     2281 ctgcgtttac gaatggaagg caatgttgat ctgatcattg ccctcacgca tcaagatagt
     2341 gaagaagact taaaaattct ggagaccgtt gagggggtgg atgtgctcat cggtggacat
     2401 accgaagggt ttgacggatt gtatgctccc gggttgatgg agcccgttga aaccatgacc
     2461 cgaccccgat cggtgtatgt gaagacgcat cgtcaaggac gaacaatcgg gcgattagat
     2521 ctgacgattt caggcggatc ggtggttcag gcgcgttcct ataatattcc agtgacggtg
     2581 gcggttccag tagagccgaa ggtccaacaa cttttaaatg aatatcgaca gcagtttgcc
     2641 ctgcatgcca cccaggtgtt gggggaggcg tcagtgcggc tccagggcga tcgaccggtt
     2701 atcaggaccc aagaagccaa tctgggcaat ttgttggccg atcgtatgcg aaacacgtta
     2761 ggcacggata tcgccttgat taatgccgga cagattcgcc gaagtctgga acccggtcct
     2821 gtaacacttg gcgatgtgct gtcggtactg ccctttgatt ccgctcttgt gaccctccat
     2881 gtgacgggag caatgctttg tgaggtttta gagcatagtg tgagccaatg gccgaatcat
     2941 tctggccggt tcttccagat ttccggtctc caggtcacgt atcaaggaaa ggctcctgtc
     3001 gggtctcgtg tgagaagcat catggttgga ggagtgccgt tggatatttc caaaacctat
     3061 accgtggcga cggacgcatt tgtggctgac gggggggacg gctatgacat gttgacgcac
     3121 gtcacacacc gaacggatca tcaaatcccc ctgagagatc ttttgctgaa tgccctagct
     3181 gaaggtccgc tctatgcgga ggctgatcat cgtatggtgt tcatgattgg gaacgaagaa
     3241 aaatggcgca atggcgttcc ttgtcttcgc tgagcggctt catgtctacg cggtgaagcg
     3301 cttcgccgtc taaatgcgca ggaaggtcgc tcattggccg ttctcaagtt ccgcgtttac
     3361 cctccttttg tttccattga tttaatcgtt gccttattct ttttatatgg cacttcggaa
     3421 gccatattgg gatcgacaat caaaatattc atgattcatc agccatctgc atacctcccg
     3481 tcttaggggc ttttcatata tccatgtctc ttcctcaaga tgctcgtgat tcacgaagat
     3541 ggtgggctga cttgctggct gtctggcggg ataccaggca gattgttctg acggctcaga
     3601 ttgccgcaat ttatgcggca attcttattc catttaaagc cggcattcca attattcctg
     3661 gttttgtgga attgcgtcca gccaatgcca tacccattgt ggcctcactc ttattcggtc
     3721 ccgctgcggc gtggggagcc gggattggta atgtgatcgg cgattgtttt ggaacgttag
     3781 gcccggccag cctcttcgga tttttaggaa attttttctt tgggtatgtc ccctatatgt
     3841 tgtggggtca cttagggtgg ctctcatctg gacaggctcc agttattaag gcttggcggc
     3901 cgatgatgga atatgtcgtg gtatgtgtca tcgcgtcagc cgtctgtgcg ggaattatcg
     3961 ggtggggagt ggaatggtta gggttgttgc cttttgtcat actggctcct gcgatttttt
     4021 taaataatat tgtcatgggc gttttcctcg ggcctccgtt attgcgcttt ctctatccca
     4081 gggttcaacg gtggcggcta cggtatgagg atatacgaaa aaataatacg ttccaattcc
     4141 agccattcgc cggaccgccg agaagatccg attcaattaa ccacgaagtg ataaaagaga
     4201 acctcagtca ggccgtcctg gaatgttgcg gactttcctt ccaatatgca tccggtgcag
     4261 accccgtcat tcggaatgtc tcattcaccc tcaggtctgg tgagatcgtc g
//
LOCUS       DATIAL010000201         2794 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1003499, whole genome shotgun sequence.
ACCESSION   DATIAL010000201 DATIAL010000000
VERSION     DATIAL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003499"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..1166
                     /locus_tag="VLA60_06360"
     CDS             438..1166
                     /locus_tag="VLA60_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HSF09018.1"
                     /translation="MTNTHYGYAILASLLAAGVTTTGLYVIRRFGTWGQYNATYFSCF
                     AAGVLISVAFLHIIPHAFHMNDQAPVYLLAGYLAFHLINRFIHAYVCDSTPDTAIGLI
                     PLLGIGLHSTLDGVVYAITFSVSVLTGTLAGIGMVLHEFPEGIVTYVLLLRSGFTPKK
                     AWLLAFLAAALTTPIGTLLAIPMIHQINETVLGALLAVSAGAFVYVGATHLLPLAERE
                     DRPYSLAAMGGGVLVALGIILTHG"
     gene            1217..1909
                     /locus_tag="VLA60_06365"
     CDS             1217..1909
                     /locus_tag="VLA60_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSF09019.1"
                     /translation="MTMKTNRVRNPLRIGHRGAAGHAPENTLAALELAIKYGVDMVEF
                     DVRRSADGALVLLHDASVDRTTNGEGSIEELSLSVLRKLDAGGGESIPLLEEALACLS
                     GRAGAMIELKVRGIAADVCAKVQVADFHGTVIYASFFHEELLPVRRLLGDAFTLALIE
                     DVPIHSTAFATAAQSTHVGLALDAMTPSLVKAFQEQGFKVFVYTVDESEDIGRMKQLG
                     VDGIISNFPDRI"
     gene            complement(1955..>2794)
                     /locus_tag="VLA60_06370"
     CDS             complement(1955..>2794)
                     /locus_tag="VLA60_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09020.1"
                     /translation="ATSIFGEGIHMTREPHRLSRRTLLKQGMGIFGLLAGGLISSSSL
                     AEILKEPTPRQSLGPFFPDEGDPIDAIRENHAIGIPISQANDQDLTFVKGRRGKAKGQ
                     VIHLRGKVLSAKTGKAIPHTVIIMWSASSSGRYNHKKDDGMLKFPHPTTGEIIHRTYD
                     AYFQYWGRAVTNEQGDYWFKTIVPGFYPIDLEAGQYRPSHLHFQLFPPEHSKLVTQLY
                     FRGDKIPNNELNQKLLPMDVVILEAGLTTIDLERVIVDYAPDASGEISDGLVGHYDFL
                     IPS"
BASE COUNT          614 a          674 c          753 g          753 t
ORIGIN      
        1 catctcaagc agacaatcca tgcttgcgtg acatgccatc aagtctttcg tttgcagaat
       61 taaagggtaa aaacatagat agcacaaccc caaaagtgga gagataatct gttgtctgga
      121 ggggccctaa ccgggttgtc attctgggcc cacggcgaag aatctgtgtc cagctagaga
      181 agccctaggg cgcagagtga tcctttgttt cagtgacgat gacatggcct tccagggctt
      241 gttgtgttgc tgaaaagcat agcattcctc tgatagtgtc caacaaatgt aggttaaagt
      301 tttaccattg aagcaaaagc gtgtgatgat tattgtttct ctttcgagta aaggatagaa
      361 ttcatttctg aatttctcat gcctgcaaat tgttagcagg tctctttcca caaccattcc
      421 agaagtgacc cgatttcatg acgaataccc attacggtta tgccatcctg gccagtctcc
      481 tcgcggccgg agtcaccacc acaggcctct atgtcattcg tcgatttgga acatggggac
      541 aatacaatgc cacttatttt tcctgtttcg cagcaggtgt gctgatctcg gtagcctttc
      601 tccatatcat tcctcatgcc ttccacatga atgatcaggc gccggtgtat ttgctggcgg
      661 gctatctggc ctttcaccta atcaaccggt tcattcatgc ctatgtctgc gatagcactc
      721 ccgacacagc cattggacta attcctctgc tcggtatcgg cctgcattcg acgttagatg
      781 gggtcgtgta tgccatcacg tttagcgtca gcgtcttaac cggaacgtta gcggggattg
      841 gtatggtctt gcatgagttt ccggaaggga ttgtcaccta tgtattgtta cttcgcagcg
      901 ggttcactcc aaaaaaggca tggctcttgg cctttctggc tgcggctctc accacaccga
      961 tcgggacatt gttggccatc cctatgatcc atcagatcaa tgagaccgtc ctgggcgcgc
     1021 tgctggcggt ttcagccggg gcttttgtgt atgtgggcgc cacgcacctt ctgcctttag
     1081 ccgagcggga agaccgtccc tacagcctgg cggccatggg aggcggcgtt ctggtcgccc
     1141 tcggcatcat cctcacccac ggctaatgtg ccccctctta cctattgttt tttagctcag
     1201 aaaaaattgc atccatatga caatgaagac taatcgggtt cggaatcctc ttcggattgg
     1261 acatcggggt gcggcgggac atgcgccgga gaatacgctg gctgctctgg aactggcgat
     1321 caaatatggt gtcgatatgg tcgagtttga tgtccggcga tcggcagatg gagccctggt
     1381 gctccttcat gatgcttcgg ttgaccggac gacaaacggg gagggaagca ttgaggaatt
     1441 gtcgttaagc gttcttcgga aattggatgc gggagggggc gaaagcattc ccttacttga
     1501 agaagctctc gcctgtctca gtggacgggc aggtgccatg attgagctaa aggtaagggg
     1561 aattgccgct gacgtgtgtg cgaaagtgca agtggctgac tttcatggga cagtgattta
     1621 tgcctcgttt tttcatgagg aactgttacc ggtgagaagg ttactaggag atgctttcac
     1681 attggcgtta atcgaagacg tgcccatcca ttccacggcc tttgccaccg ccgctcaatc
     1741 gacccatgtg ggattggcgt tggatgctat gacgccttcg cttgtaaagg cgtttcagga
     1801 gcaagggttt aaggtgtttg tctatacggt cgatgagtct gaagatattg gccgaatgaa
     1861 gcagctggga gtcgatggga ttatttcaaa cttccccgac cggatctagt ccaggttgca
     1921 ccaatttgcg tgttcaaaaa aactggattt cctactagct tggaatcaaa aaatcgtaat
     1981 gaccgaccaa gccatccgaa atctctcccg atgcgtccgg ggcatagtcc actatcaccc
     2041 tctctaaatc gattgtggtg agcccggcct ccagaatgac cacatccatt ggcagtaatt
     2101 tctggttcag ttcattgttg gggattttgt ctccccggaa ataaagctgt gtgaccagtt
     2161 tcgaatgctc gggagggaac agttgaaagt gcaggtggga agggcggtac tgtccggctt
     2221 ccaggtcgat ggggtaaaac ccgggaacaa tggttttgaa ccaatagtct ccctgctcat
     2281 tggtgaccgc tcgtccccag tattgaaaat acgcatcgta ggtccggtga atgatttctc
     2341 cggtcgtggg atggggaaat ttcaacatac cgtcatcctt tttgtggttg tatcggccgg
     2401 aggacgaggc actccacatg atgatgacag tgtgaggaat cgcttttccg gtttttgcgc
     2461 tcaggacttt tcctctcaga tggatgacct gccccttggc ttttcccctg cggcctttga
     2521 caaacgtgag gtcctgatcg ttcgcctgac taatgggaat gccgatggcg tggttttcgc
     2581 gaatggcatc tatcgggtct ccctcgtcgg gaaaaaaggg ccccagggat tgacgcggag
     2641 ttggttcctt gaggatttct gcaaggctgg aggagctaat gagacctccg gctaacaagc
     2701 cgaagatgcc cattccctgt tttaacaggg ttcttctgga caagcgatga ggttctctcg
     2761 tcatatggat tccttctcca aatatggaag tggc
//
LOCUS       DATIAL010000202         8574 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1111682, whole genome shotgun sequence.
ACCESSION   DATIAL010000202 DATIAL010000000
VERSION     DATIAL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111682"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VLA60_06375"
     CDS             complement(<1..432)
                     /locus_tag="VLA60_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09021.1"
                     /translation="MATSPFFTPDELALLADQDFFRKKAEISVKIKQVLEHLHDRLKA
                     EIRLHHLLAPEGFDPQVSQFVKGEHLENFPYQYVDFPRFYTRENKFAFRSLIWWGHHM
                     VFALIVEGPLVKQYRRNLFNRYSEVADHHLCLCLSPSLWEWK"
     gene            541..1317
                     /gene="xth"
                     /locus_tag="VLA60_06380"
     CDS             541..1317
                     /gene="xth"
                     /locus_tag="VLA60_06380"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HSF09022.1"
                     /translation="MKIATYNVNSIRKRIGILHKWLQQHNPDVMCLQETKVQDHEFPL
                     QAFSDLPYVINFCGEKSYNGVAIFSRASPETVAFGFEDGELPADPTRLVRVVVNGIMI
                     INTYVPQGFAIDSPKYTYKLRWFQRLRRYFSRVVSPGQSVIWCGDMNVAPEPVDVHSP
                     EKHLKHVCFHEEVRRAYQETVSWGFVDVFRHHFPRRQQFTFWDYRRPGALEANRGWRI
                     DHILVTPPLLAYSHTVKVDVQPRRAESPSDHTVLCAEFSL"
     gene            complement(1722..2312)
                     /locus_tag="VLA60_06385"
     CDS             complement(1722..2312)
                     /locus_tag="VLA60_06385"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HSF09023.1"
                     /translation="MVPTQMFLTRGVGVHKEKLASFEEALRSAGIAYCNLVTVSSILP
                     PDCKIISRKRGEKLLNPGEITFCVMARSETNERNRLISASIGVAIPSGRKVNYGYLSE
                     HHAYGETDEEAGEYTEDLAAQMLATTLGIKFDPNVAWKEREQVFKMGKDIVRTQNITQ
                     SAIGKPNLWTTVVACAVFIPLENITDDPKPRRKKSK"
     gene            complement(2559..3992)
                     /locus_tag="VLA60_06390"
     CDS             complement(2559..3992)
                     /locus_tag="VLA60_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF09024.1"
                     /translation="MTVATAKPILIGGQWVQTSSAEPVRNPYSNDVVAMVCQAEPEHI
                     HQVTNSAKRVSHQCAGIPAHARAKALLQIAAGLSTRREMFAATICQEAGKPITDARRE
                     VDRAIQTFRLAAEESTRIPGEIIPMDLTPGGEAYSGSVQRFPIGTVLGITPFNFPLNL
                     VAHKVAPCLAAGNPMVLKPAPQTPLTSLMLGEVFLTTELPPEMLTIIPCSNTLAEQMV
                     VHSNFQALSFTGSMTIGWMLKGKAGYKRVLLELGGNAGVIIEPDANLDVAIDRCVAGG
                     YGYAGQTCISVQRIFVHESRYDDFLKTFVERVRSLPMGDPSLENTVVGPLINEQAARR
                     VEAWIQEAVSHGATVMTGGTRKDSFIEPAVLTHVNNNMKVCCEEIFGPVVTVTPYADL
                     EEAFALHNDSDFGLQTGIFTSNIDTMYQAYSRLEVGALLVNEIPTFRADHMPYGGIKQ
                     SGLGREGVRYAIQELTEPKLLLVKRPS"
     gene            complement(4025..4285)
                     /locus_tag="VLA60_06395"
     CDS             complement(4025..4285)
                     /locus_tag="VLA60_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09025.1"
                     /translation="MTESQSFWPVECAQGEPDLFVCLTCFDEVFKAKMPVDGCPGCGA
                     IAAFEPFSLDAIREWGTENLIQKAERLPSSSHPGSDQPASSI"
     gene            complement(4403..5131)
                     /locus_tag="VLA60_06400"
     CDS             complement(4403..5131)
                     /locus_tag="VLA60_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSF09026.1"
                     /translation="MIAHSQGVMMNIARSLKSTVLLAILLGTLLMSPTIHGKTPDFLI
                     QDDDVVRGDPKAPITLLEYSDFTCGFCEKFFHETFPKLLSEYIETGKVRFVYRDFPRG
                     MGSPLRAADAARCAGEQNAYWPMHDRLFNSGGQLAPDNLKQYAKELNLHGEQFSECLA
                     SHRYMKDIEKDLQDAGSLGIRGTPAFVLFPTTLPEDVHLILIPGAFPYETFKEEIDKL
                     LKLHGGSTSSLSLPVLSSTHIKGA"
     gene            complement(5192..6286)
                     /locus_tag="VLA60_06405"
     CDS             complement(5192..6286)
                     /locus_tag="VLA60_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HSF09027.1"
                     /translation="MTTRTFHDGESDGLEALEALDPETIESFSDLLSAMKKTAFGGRR
                     LGDAFEILNKMVEDPDCTVILTLSGAMTIAKMGKIICSMIDRNMVQAVVSTGALIAHG
                     LSEAVGKTHYKYHPSMTDTELYEKGYNRVYDTLEMESNLNHVERVVSQTLKRMTPDTP
                     ISSEILTREIGKTLHDHYEGPGILKSAYEQGVPVYIPAFTDSEMGLDVSIWAMKERRQ
                     SQGTPPSPYLSDQETWQVLQQSRPAFNPFLDLNSYTEKLLSAKRLGIFTIGGGVPRNW
                     AQQTPPYIDILNLRMGTQLTPPRYQYGVRICPEPDYWGGLSGCTYEEGVSWGKFVPRK
                     EGGMFAEVLSDATVVWPLLMMGLLEKARRR"
     gene            complement(6407..6484)
                     /locus_tag="VLA60_06410"
     tRNA            complement(6407..6484)
                     /locus_tag="VLA60_06410"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6446..6448),aa:Glu,seq:ctc)
     gene            complement(7133..>8574)
                     /locus_tag="VLA60_06415"
     CDS             complement(7133..>8574)
                     /locus_tag="VLA60_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HSF09028.1"
                     /translation="SSPPPETAPAITAAPMETELPVTVVHAADSPTVVNVSTPLLASN
                     ETFVKIAKEAMASVVNISATKRTVQAPGTNPLLEDPFFRRFFGEEFERRFKQPRDRQE
                     QGLGSGVIVSDDGYIVTNNHVVEQADDLMVLLGDKRKLPATLIGTDPKTDLAVIKIEA
                     TGLSTLPWGNSTTLEVGELVLAVGNPFGLNQTVTMGIISAVGRANVGIVDYENFIQTD
                     AAINPGNSGGALVNLQGQLIGINTAIFSRTGGYMGIGFAIPSQMVKSVMQSLIGHGKV
                     IRGWLGVSIQELSPDLATQFEVPDTQGALVGDVFSESPAGQAGLKRGDIIRTYNGVTV
                     KDPNHLRTLVAETTPKSSIPLAVWRNGKIVNLSVSITEMPKDTAALTEMAPSSVSGNH
                     ALSGLSVEPVQPGQTRDGQGVVVTQISPNSPAERAGVQPGDVILELNRSPIGSVKDFE
                     QLVKRLEAGASVLVLLQRGKGAIFLTIKP"
BASE COUNT         2090 a         2077 c         2313 g         2094 t
ORIGIN      
        1 tttccactcc cagagagatg ggctgagaca caggcagaga tgatggtctg ccacctccga
       61 gtatcgatta aataaattgc gtcgatactg cttcacaagc gggccctcaa ctatcagagc
      121 gaagaccatg tggtgccccc accagatgag ggaacggaac gcgaatttgt tctcacgcgt
      181 ataaaatctt gggaaatcga catattggta ggggaaattt tccagatgtt cgcctttgac
      241 aaattgactg acttgagggt cgaacccttc aggagccaaa aggtgatgta agcggatttc
      301 tgccttgagc cggtcatgga ggtgttccag gacctgtttg attttgaccg aaatttcggc
      361 tttcttccga aaaaaatcct gatcggccaa gagggccaat tcatcaggag tgaagaaagg
      421 actggtagcc ataagttcat gtccctcaca gacccaagta tcggttgtgg agaagtatcc
      481 gcctttgttc ttttcgagga atgtctgcag cgtgagtggt aaataggagt ttcaagaaga
      541 atgaaaattg cgacctacaa tgtcaattca attcgaaagc ggatcggtat tcttcacaag
      601 tggttacagc aacacaatcc cgatgtgatg tgccttcaag agactaaagt acaggatcat
      661 gagtttcccc ttcaagcatt ttctgactta ccctatgtca tcaacttttg tggtgagaaa
      721 tcctacaacg gcgtcgccat atttagccgg gcatctccgg aaacggtggc atttggtttt
      781 gaggacgggg aactaccggc ggatcccact cgcctggttc gagtggtggt caatggcatc
      841 atgattatta atacctacgt tccacaggga tttgcgattg attcgccgaa gtatacctac
      901 aaattaaggt ggtttcagcg attgaggcgg tatttctcgc gtgtggtctc accaggacaa
      961 tccgtgattt ggtgtggaga tatgaatgtg gcccctgaac cggttgacgt acatagcccc
     1021 gaaaagcacc ttaagcatgt gtgtttccat gaagaagtgc gtcgggccta tcaggagacg
     1081 gtgtcctggg gattcgtgga tgtgtttcgg catcattttc cgcgccgtca gcaatttacg
     1141 ttttgggatt atcgacgtcc aggtgcttta gaagctaacc gtgggtggcg aatagaccac
     1201 attctggtta ccccgccgct tcttgcctat tctcacacgg tgaaggtgga tgttcagcct
     1261 cgtcgtgcgg aaagcccctc ggaccatacc gtcctgtgcg ccgaattctc actgtgagag
     1321 tgttgaataa tactaatttc caagggcata ttgacccaca ggcacgttgc atatcagagg
     1381 cctcggggcg gtttaaaaaa gttcgtccag tcctgccatg agtcatgtcg aagggaaggc
     1441 cgcagtcatt tttgcgcgca gagcctactt ccagtacgcg agcacggaaa aatggcgacc
     1501 tgcctgcgcg aagccgctcc ggcgaaggca gggacgccgc tgggggtttt tttcaacagt
     1561 cccactgtga gggaaaaggg cggctcctgc agaaccgatt gtggtaaagg aggggtaatc
     1621 ctactgcata aaaagatgtt cgaatgtggt cttgtcaggt cgctaaagac tcgagaaaga
     1681 gaagcaatta tttaccagaa tggtttaccc aatccacgac cctattttga ttttttccga
     1741 cggggctttg gatcgtcagt gatgttttca agtggaataa agactgcaca tgcgaccacg
     1801 gtcgtccaga gattcggttt tccgatagca gattgagtaa tgttctgggt tcgaacaata
     1861 tcctttccca ttttgaatac ctgctcacgt tctttccatg caacatttgg gtcgaatttg
     1921 atgccaagtg tggttgcgag catttgggcc gcgaggtcct cggtatattc tcccgcctct
     1981 tcgtccgttt ctccataggc atgatgttcc gacaagtagc cataattaac tttcctgccg
     2041 gagggaatgg ccacgccgat tgaggcagaa attaaacgat tccgctcgtt cgtttccgat
     2101 ctggccatga cgcaaaaggt aatttcgcct ggattcagta acttttcgcc gcgttttctc
     2161 gagatgattt tacaatctgg aggaagaatg gaggagacgg tcaccaggtt gcaatacgca
     2221 attcccgcgc tccggagggc ttcttcgaaa gatgccagtt tttccttgtg gacgcccaca
     2281 cctctggtga gaaacatttg cgttgggacc atcgcgactc cttcgtcaat cagttagtgg
     2341 tggattggga taggcaggag atgactaggt gatcgtcgaa tctgattcac aacctcgctc
     2401 tccaatctgt ttgcgcaaac tctatatggg aaagcctgga cgattcggac ggtcacggga
     2461 aagaacaacc ttgccacgta cgccggtatt tttaccaaga gtggttggca aggtgcaaga
     2521 tcgtgaggac tgaacgtgca agaaaaaata aggaggcctc aagaaggcct tttgaccagc
     2581 aacaatttag gctcggtcag ttcctgaata gcataccgta ctccttctcg ccctaatcct
     2641 gactgtttga ttcctccata aggcatgtga tcggcccgaa aagtcggaat ttcattcacc
     2701 aggagtgcgc cgacttccaa ccgtgagtat gcttgataca tggtgtcgat gttgctggtg
     2761 aaaattcctg tttggagtcc gaaatcagaa tcattatgta gggcaaacgc ttcttcaagg
     2821 tcggcatagg gtgtgacggt caccaccggc ccgaaaattt cttcacaaca gaccttcatg
     2881 ttgttgttga cgtgagtcag gacggcaggc tcgatgaatg aatcctttcg tgtcccgccg
     2941 gtcatgaccg ttgctccatg cgaaacggct tcttggatcc atgcctcgac tcggcgggcg
     3001 gcttgttcgt taatgagggg accgacgacg gtgttctcga gggagggatc gcccatgggc
     3061 aaagatcgaa cccgctcgac aaaggtcttg aggaagtcgt cgtaccgtga ttcatggacg
     3121 aagattcgtt gcacagagat acaggtttgt ccggcatagc cgtaccctcc cgccacacaa
     3181 cggtctatgg ccacatcaag attggcatcc ggttctatga tgaccccggc attccctccc
     3241 agttcgagca gaacccgttt gtatccggcc tttcctttca gcatccaccc aatcgtcata
     3301 gagccggtaa agctcaaggc ctggaaattg gaatgaacca ccatctgttc tgccagggta
     3361 ttggaacacg ggataatcgt cagcatttcc ggaggcagtt ctgtcgtcaa aaagacttca
     3421 cccaacatga gcgatgttaa cggggtttgc ggggctggtt tcaataccat cggatttccc
     3481 gctgccaggc aaggggccac tttgtgggcc accaagttta gggggaaatt aaatggggta
     3541 atgccaagaa ccgtcccgat cggaaagcgt tggacagatc ccgagtacgc ctcccctcct
     3601 ggggtgaggt ccatcggaat gatttctcca gggatgcggg tgctttcttc tgcggccaaa
     3661 cgaaaggtct gaatggcccg atcgacttca cggcgtgcat cggtaatcgg tttcccggcc
     3721 tcctgacaaa ttgtggccgc aaacatttcc cgtctggtgg atagtcctgc cgcgatctgc
     3781 aggagcgcct ttgctctagc atgtgccggt atcccggcac attggtgcga gactcgtttg
     3841 gcagagttgg tgacctggtg gatatgctca ggctctgcct ggcagaccat ggccactacg
     3901 tcattggaat agggattacg taccggctcc gcagaggaag tctggaccca ttggccgcct
     3961 ataaggattg gctttgccgt ggccacagtc atgaaaagct gaaaacctcg tgcaatagcg
     4021 gtggttagat tgatgaagct ggctggtcag aaccgggatg ggatgaagaa ggcaaccgct
     4081 cagctttctg aatgagattc tccgttcccc attccctgat cgcatccaaa ctaaacggtt
     4141 cgaatgcggc gatggctcca caacccggac atccgtcgac cggcatcttg gctttgaaaa
     4201 cttcatcaaa acaggtcaag cacacaaaca aatcaggttc accttgcgcg cattcaacag
     4261 gccagaagga ttgggattca gtcatgactc aaaaccattc tgtgggtaaa aacatgaatt
     4321 tcagcattgc cactgcaaat gtacacctaa tcacgctgtc agatcaatga ggaattgcgt
     4381 ggagagcgaa tgcatgaagg atttatgctc ctttgatgtg agtagaggac agtactggca
     4441 aagacagtga agaagtcgag cctccatgaa gcttgagcaa tttatcaatt tcttccttga
     4501 acgtctcata gggaaaggcg ccggggatta aaataagatg aacatcttct ggcagggttg
     4561 taggaaagag gacgaatgcc ggcgtgcccc gaattcccag ggaacccgca tcctgtaaat
     4621 ctttctcaat atctttcatg tagcgatggg aagccaagca ttctgaaaat tgttctccgt
     4681 ggaggttgag ttccttggca tattgtttga gattatccgg agccaactgg cctccactgt
     4741 taaagagccg atcatgcatg ggccagtagg cgttttgctc tcctgcgcat cgagcggcat
     4801 cggctgctcg aaggggactg cccatgcctc tcgggaaatc ccgatagaca aaacgcactt
     4861 ttccggtctc aatgtactcc gaaagcaatt taggaaaggt ctcatgaaaa aacttttcgc
     4921 aaaagccaca cgtgaagtcc gagtattcca acagggtgat gggagccttg ggatcccctc
     4981 gcaccacatc atcgtcctgg atcaaaaagt caggagtttt cccatgaatc gtgggactca
     5041 tgagcagggt cccaagaagg atggcgagca atacggtcga tttgagagat cgggcaatgt
     5101 tcatcatcac tccttggcta tgggctatca ttgtggcatt cctggcggac cgtgtcgaga
     5161 gaagaagggt ggtcgtgccc aggggttgaa tttatcggcg acgggctttt tccaggagcc
     5221 ccatcatgag aagaggccaa accactgtgg catcactcag gacttcagca aacattcccc
     5281 cctcttttcg aggcacaaat tttccccatg atacaccctc ctcatacgtg catccgctga
     5341 gaccacccca gtagtcaggc tccgggcaga ttcggacacc gtactgatac cgcggagggg
     5401 ttaattgtgt acccatccgg aggttgagaa tatcgatata gggtggcgtt tgttgtgccc
     5461 aattacgagg aactccgcct ccgatagtga aaattcccaa tcgtttggca gacaacagtt
     5521 tttccgtata actattaaga tctaaaaacg gattaaatgc cggacgagat tgctgtaaga
     5581 cctgccaggt ttcctgatcg gagagatagg gggatggggg tgtgccttga gattgtcgtc
     5641 tctccttcat ggcccaaatg gagacatcca gccccatttc agaatcggta aatgcaggaa
     5701 tatagaccgg gacgccctgc tcataggcgc ttttcaatat ccctggtcct tcgtaatggt
     5761 catggagagt cttcccgatt tcgcgggtca gaatttccga ggagatgggt gtgtcagggg
     5821 tcatacgctt taaggtttgg ctgacgaccc gttcgacgtg gttgagattg gattccatct
     5881 ccagcgtgtc gtacacgcgg ttatagccct tttcgtaaag ctcggtatcc gtcatggatg
     5941 ggtgatattt atagtgtgtt ttgcctacgg cttcactcaa gccatgggca atcaaagccc
     6001 ccgtggatac cacggcctgc accatgtttc ggtcgatcat gctgcagata atctttccca
     6061 ttttcgcgat ggtcatggcc ccagacaagg ttaagatcac cgtacaatcg ggatcttcga
     6121 ccattttgtt caagatctca aaggcatcgc ctagacgtcg cccgccaaag gcggtctttt
     6181 tcatggccga gagaaggtcg gaaaaagatt caatcgtttc cggatccaga gcctccaggg
     6241 cttccaggcc gtcgctttcg ccatcgtgaa aagttctcgt agtcatcaca gatccttacg
     6301 gcacatagat gaaagaaata accagacggg agtacgagga gtctgattct aacgggagcg
     6361 ggtgaaaaca actaggaagg ggcgaacatc agctcctaat agcgattggt gatcccaacg
     6421 ggatttgaac ccgtgtctgc accttgagag ggtgccgtcc tagaccagcc tagacgatgg
     6481 gaccagtctg tgtctgttta tcaattgaaa ttcgtactct ctgcccttat caggcatgtc
     6541 agaaatgtac cattagggac ttatggcatg caacgaaaaa agggcagatg acagggagca
     6601 tagacctaca atcacctgtg ttgacaaggg aggaaaggcg caacaacaaa aatatggctt
     6661 ttgaatcaga tatcagagga cggggagtgc ggagtatgga tagttaacag gccccgcatg
     6721 aaggggtgaa gtccacaatg gtaagggtac tgcccggggg ctaaccgggt taaggcaaac
     6781 tgatgatcgg gacgaatgac gcctgagtca aatgagcact gggagcggga ctgacagtcg
     6841 tctgccacga tggaatgggc ttctctagtt tgattgttcc acgtgagggt tgtacccagg
     6901 cggatcatca ggtggttcgg agaaaaaaat ggcggttcag cttggatggc aatctgcgga
     6961 gaagaggccc acagaattgg ttgcgtggaa acaggggatg ataagagaaa agccaagaag
     7021 cagaacagtt ggcttatttg aaggaatgtt ctggatgaac aaggtggtgt catggttgtt
     7081 cggctctcaa caaggtgtaa ggtctggcga gggagaggct ctggtccaat cattatggtt
     7141 tgatggtcag gaaaatggcg ccctttcctc gctgcaagag cactaagacg gaggctccag
     7201 cctccagtcg cttcacaagc tgttcgaaat ccttcacgga tccaatggga gagcgattca
     7261 gttccagtat tacatccccc ggttgaacgc ctgcccgttc agccggagag ttcggtgaga
     7321 tttgggtcac gaccaccccc tggccatcgc gtgtttgtcc gggttggacg ggttctactg
     7381 aaagccccga gagagcgtgg ttacctgaga ccgaactagg agccatttcc gtcaatgcag
     7441 cagtatcttt aggcatttca gtgatggaaa ccgaaagatt gacaattttg ccgtttcgcc
     7501 acaccgcaag aggaatcgaa ctcttcggtg tcgtctcagc cacaagcgta cgcaaatggt
     7561 tcggatcctt cacagtgaca ccattgtagg tgcggataat atcaccacgt tttaaaccgg
     7621 cctgccccgc cgggctttcg ctaaagacat ctcccaccaa tgccccctgg gtatcaggca
     7681 cctcaaattg ggtcgcgagg tccggcgaca attcctgaat cgatacgccc agccacccac
     7741 gaatgacttt gccgtgccca atcaggcttt gcatcacgct tttgaccatt tggctgggga
     7801 tggcaaagcc aatgcccata tagccacccg ttcgggagaa gatggcggta ttaatgccga
     7861 tgagttgacc ttgtagattg acgagagcgc cgccggaatt gcctggatta atggcggcat
     7921 cggtttgaat gaaattttca taatcgacta tgccaacatt ggcacggccc acagcgctga
     7981 taatccccat cgtgaccgtt tggttcaatc caaagggatt gccgaccgcc agcaccaatt
     8041 ctccaacttc cagtgtcgta gaatttcccc agggtagagt ggataatccg gtggcttcaa
     8101 tcttgatcac cgctagatcg gttttgggat ccgtgccaat aagtgttgcc ggaagtttcc
     8161 gtttatcccc caggaggacc atcaagtcat cggcctgttc taccacgtgg ttattggtga
     8221 cgatgtatcc gtcatcgctg acaatcactc cggaacctaa gccttgctcc tgccgatcgc
     8281 ggggttgttt aaaccgccgt tcaaattcct ctccaaaaaa cctccggaaa aatggatctt
     8341 caagcagggg atttgtgccg ggagcttgga ctgtgcgttt agttgcggaa atattcacga
     8401 ccgaggccat ggcctccttg gcgattttga caaaggtttc gttagaagcc agaagggggg
     8461 tcgagacgtt aaccacggtc ggcgaatcag ctgcatggac gaccgttacg ggcagctccg
     8521 tctccatggg ggctgcagtg atggcagggg cagtctcggg agggggggag gatg
//
LOCUS       DATIAL010000203         3034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1111835, whole genome shotgun sequence.
ACCESSION   DATIAL010000203 DATIAL010000000
VERSION     DATIAL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111835"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(813..2285)
                     /locus_tag="VLA60_06420"
     CDS             complement(813..2285)
                     /locus_tag="VLA60_06420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF09029.1"
                     /translation="MGLTLYYVFLIALYTRNHGLDPVPLCCFEESALPCVTIQLPIRN
                     EGQLAARVIRRAAALEYPSDRLEIQVLDDSDDETSTIIQREVDRLRQQQAELDINVIR
                     RTTRDGFKAGNLKNGFPMAKGEMLAIFDADFLIPTDFLRRTVHFFTDPLVGIVQARWS
                     YLNRETSCFTEFQATKLDTHQMFEQAARARAGLWIHFHGSAGIIRKAAVEDAGGWNCL
                     SEVEDVEFSIRANIKKWQLVYLDQFKVPSEVPETMTGFLVQQMRWKRGWIRVLKYYAG
                     DIWKSDFPLSIRLDFLVRLFGSFGPSLSLPFTLGALPAFLISAQYGLQWFVYLEFSLL
                     LICSLCTRLAEGHYVTGNIDSEIPMRKFRLSSFIPIGFVVNLGMMWVQTQSTLEGLGN
                     VQSWDVTPKSHKHLVQQKVPLPGYIVGTVLMCIYATTFSVWSFWTGHFLASGFYMLTM
                     VGAGWITMSFLLERFSFKRSLSESFQEVKPRNYKSVMHTE"
BASE COUNT          830 a          726 c          695 g          783 t
ORIGIN      
        1 catggggatg atacgcatgc gaagcatgac tcagatggat gcaggaaagc cagcctgtgc
       61 gacggtttaa caggcaatag atcagaatgt aatggtgtaa ccggcaccga ggccactttc
      121 ccaagccttc cttagcgcta aatcccgtta gcatcattat gccaaaaagc aaggtaagga
      181 aacccacctg taggtgataa tttcttgagt cacagccgat tttccttctg gatatttacc
      241 aatgggggcc aattcaatca ggaaaaggcc tatatgaaac agacctagtc ccacaatatc
      301 tccctgacaa aagatacgac atgcagaaaa accaccccca aaaatgcttc ttccatttca
      361 tcatctggca ttatttgtac ggcctgctaa ttctcctgaa ctagtactac ccctccttgt
      421 tttcgctgag gaagaattga gggcacgtta attctttttt tttggactcg aaaatgtaat
      481 ccccatcttt ttgatacgga aggtggcagg tttgtggccg aggagttcgg ggatcccgcc
      541 ggtgccggaa ccacattgaa tccatactag agatctcatc taaatcgcga acctttactt
      601 ccctgggaca atttcgggtt agtggccgca atgctgaagc aatttacctt tcgtcttgtc
      661 tgagcccgat cccgttcgta atcgcttcca tcgtaaggtg cgtagtgtgt tgcatgacct
      721 ctaagggaac ttctttaact ccatccacaa aaactgtgca gcaggggatt gatgggagcg
      781 agccggtctt tctggggaat ttggtcgcag gttcactctg tatgcataac gcttttatag
      841 ttacgaggct tcacctcctg gaaagattcg gagagcgatc tcttgaatga gaaccgttca
      901 agcaagaatg acatggttat ccagccggct cccaccattg tcagcatata gaatccactt
      961 gccaggaagt gccccgtcca aaaggaccaa acagaaaacg tcgttgcata tatacacatg
     1021 aggactgttc cgacaatata gccaggcagg ggaacttttt gttgaaccag atgtttatgg
     1081 gattttggtg tgacgtccca actctggaca tttccgagac cttcgagagt gctctgggtt
     1141 tggacccaca tcattccaag attcacaaca aacccaatcg gaataaatga agagagccgg
     1201 aatttccgca tcggaatttc agaatcgatg ttccctgtga cataatgtcc ttcagccaat
     1261 cgtgtacaaa gagagcaaat gagcagcagt gagaattcca gatagacgaa ccattgaaga
     1321 ccatactgag cacttatcag aaatgccgga agtgcaccta aggtgaaggg cagagagagt
     1381 gatggcccaa aactaccaaa caggcgcacc aaaaaatcga gacgaatcga gagaggaaaa
     1441 tcacttttcc agatgtcccc ggcataatat tttagtactc gtatccatcc tctcttccaa
     1501 cgcatctgtt gaacaaggaa tccggtcatg gtttccggaa cctctgatgg caccttgaac
     1561 tggtcaaggt aaacaagctg ccacttcttg atattggcgc ggatactgaa ctcgacatct
     1621 tcgacttcgg ataggcaatt ccacccacct gcgtcctcaa ccgctgcttt gcgaatgatc
     1681 cctgcgcttc cgtggaaatg aatccatagc ccggcgcgag cccgcgcggc ctgctcgaac
     1741 atctggtgtg tgtccagttt tgtggcttga aattccgtaa aacaggacgt ttctcgattc
     1801 aggtaggacc atcgggcttg gacaataccg accagggggt ccgtgaaaaa atgaacggtt
     1861 cgccgaagaa aatccgtcgg tatgagaaaa tcggcatcaa atatggccaa catttctcct
     1921 tttgccatgg gaaagccgtt tttgaggttc ccggccttaa atccatcccg tgttgttcgt
     1981 ctgatcacat tgatatccag ctctgcctgc tgttgacgca agcggtccac ttcccgctga
     2041 ataattgttg atgtctcatc atcggagtcg tcgaggactt ggatttcaag tcggtcactt
     2101 ggatactcca gtgccgccgc acggcggatg acgcgcgcag ccagttggcc ctcgtttcga
     2161 attggaagct gtatcgtgac acatgggagt gcgctctctt caaaacaaca gaggggtaca
     2221 gggtccaaac cgtgatttcg tgtatacaat gcgatcagaa atacataata gagggtcagt
     2281 cccaatagga agactccgca gacaaccggg acaaaaaagg ctaacgtggc tgctatgcta
     2341 agaataagtg acccccctaa tggtcaaccc cctaaaggag tgaaatgtct gaaaaattag
     2401 gaattccttc tgacaaaaga aaaccggcat taagcagtaa cagattttta aatcgcctct
     2461 tgtaaaaatg tctttctcct ttaggctcag aacgtttttc tatcccacaa aaatacctcg
     2521 ttttaactta taagtccgga aaggaagtta tgtttttaac tattcttcct ttggctaata
     2581 aattttgaga ggcatcaaga gcaatgaata gaatggggcg atcccaccaa ccggttcctt
     2641 ccctattggt tttcggcaat cggtaacata taggcaaaac tttaaggcct ttgatgagag
     2701 cctattccgg gattaccggt tccttggcgc cggacaggtg tacctccaat tctgaaagca
     2761 ccagtcttgg aaggggtgga gtaattatag agcgtcgcat tgacaactcg aattcgcctg
     2821 ccaatataca ttaagggtgt tatggtcaag ggcaatccct tcacagctga aatcctctga
     2881 ctcctcgtgt tgtgtcaata atttgacatt ggaacttccc tcattcctag atcaacaaaa
     2941 ttaatgctgt ctggatccat gccaattata tggtatcaat caatccaaat gaggttgctg
     3001 ttcatcacga tcagtgcggg tggaattctc tttt
//
LOCUS       DATIAL010000204         4738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_466976, whole genome shotgun sequence.
ACCESSION   DATIAL010000204 DATIAL010000000
VERSION     DATIAL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466976"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..939
                     /locus_tag="VLA60_06425"
     CDS             163..939
                     /locus_tag="VLA60_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09030.1"
                     /translation="MSKIYDALQIAYGEKLAAGNESKKEPIEVPSVSIPSPSLLQVRN
                     TTSCPRLYNESELMVMAQNIAALLPSPDKNVIQFIGSRRGEGTSTLVREFALVSSQHS
                     NKPVLLVEADLMQPCQYQAFGIEAKSPLDHVLKDGKALEGVVSQVEQSNLFLATLSSK
                     VQSALTTRTFYNPTDMWKIVREQFSLILIDSSPANVTADSLALCETVDGIVLVVEAEQ
                     TRSAVAKKVRDQILMQEGNLLGMVFTKRHLYIPESLYKFI"
     gene            complement(1691..2365)
                     /locus_tag="VLA60_06430"
     CDS             complement(1691..2365)
                     /locus_tag="VLA60_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HSF09031.1"
                     /translation="MPYPNPFAARYAILAILCGLVPLGGCSQDYTTPLGEAVANEPPP
                     QGLPSNQQSSTFDITKSKPASQIYDFIMEDIDNKPRSLNEFKGQVIMIVNTASFCGNT
                     PQYAGLQTLYERYRDQGFTILAFPANDFGQQEPGDNKEIAEFCYTKYSLEFPLFSKIT
                     VVGEQKHPLYRYLTEDTNFTGEITWNFQKFLINRKGTVIARYDPRQKPLSPEIVNDIE
                     NTLQSS"
     gene            complement(3136..3471)
                     /locus_tag="VLA60_06435"
     CDS             complement(3136..3471)
                     /locus_tag="VLA60_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSF09032.1"
                     /translation="MIANIEDREFDGIIEKSTVPVLVEFWQPGCGHCRALAKELEHIQ
                     KEFGKRLLILAMNVQENFLIPGELEIQSLPTLALFVNGEFKQFIGGVGKKDLILEQVF
                     PWLEDSHQG"
     gene            complement(4091..>4738)
                     /locus_tag="VLA60_06440"
     CDS             complement(4091..>4738)
                     /locus_tag="VLA60_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF09033.1"
                     /translation="SPQGLALSISHVRGLPPPWPPTLELNMELAPSEKWAIVCVTETQ
                     RLTVSRLLAGLDHPPQGSIRYNGIDVRDLHPDDVNETRGLVLSRHMTLFESTLMENIT
                     LRRKNLATEDLLWVLDLVGLQEEIEHIPDGLQGMVQYGGKNFSPSQTIQILLARVLIA
                     RPKLVIVDGGLHEIFASRRESILNAICDTKYPWTVVIVTTDSNIKKFVQHSIVLE"
BASE COUNT         1264 a         1089 c         1066 g         1319 t
ORIGIN      
        1 ggggataatt tgagtcaaat tgccaatgaa gcctatggtt cctcaagccg gcaatatgtg
       61 gaatggctcc gtcggcataa cccacagatc agggatcccg atatcattct tccaggtcaa
      121 aagattgtat tacctgagta cgtgaaagaa taagaccaca ttatgagtaa aatttacgat
      181 gctctgcaga tcgcctatgg agaaaagctg gctgccggca atgagtcaaa aaaggagcca
      241 atagaagttc cttctgtgtc gatcccgtcc ccctctttat tacaagttag aaatacaacg
      301 tcttgtccaa ggctttataa tgaatcagaa ttgatggtca tggcacaaaa tattgccgca
      361 ctgcttccaa gccctgacaa aaacgttatt cagttcatcg ggtcaagaag gggagaaggc
      421 acctccacac ttgttcggga atttgctctg gtttcctctc agcacagcaa taagcctgtt
      481 cttcttgtcg aagcagatct tatgcagcct tgtcagtatc aggcattcgg gatcgaggct
      541 aaatcccctc tggatcatgt attaaaagat gggaaagctc ttgaaggtgt cgtttctcag
      601 gttgaacaat cgaatctttt tctagctaca ttatcttcta aagttcagtc agctctcaca
      661 actcgcacat tttacaatcc tactgatatg tggaaaattg ttcgggaaca gttttccctt
      721 atactcatag attcttctcc ggccaatgtc accgcagata gcttggcgct ttgtgagacc
      781 gttgatggta ttgtgctggt tgtcgaagcc gaacaaaccc gttcggctgt cgcaaaaaaa
      841 gtcagagatc agattcttat gcaggaagga aatctgcttg gcatggtgtt tacgaaacga
      901 catttataca tcccggaatc tctctataaa ttcatttaga tttttctttt tttctaatcc
      961 tgcttttttc tcctcaatca tgtgattacc tattactact tccattgttt cctttgagaa
     1021 atacatcttc agtaggatct gtcctattcg aacgtcttcc ctttccaata tttcaaattt
     1081 ctgccattgg tcttagagta tcttcccaat tatagaaagt tcatgttgga ataggcctca
     1141 ctttattgag atagccgaac atctccaatc tttttatggg gcagaagaca aaaacacgtc
     1201 acgccttagg aaaatgtagc atccaaagag caggctaatt agaatcatgg ttgtccgggt
     1261 gctatggaat ccttcgatta cttgggtcgt caactcactg aggcagccga gaccaagata
     1321 cagtatgtag cccccgaatg cccataagct tttttttaaa aaaccatatc ccaataccca
     1381 atggataaag gggtgttggt attttacaaa ttctcctgcc cctccgctga atgttgttcc
     1441 gaaaaccttg agtgcatagt gagggtagct ggctatccaa actaaatcca aggtgcctgc
     1501 caggagtaat agcatcccaa acatttgtat atcccgtccc tggtgcagga aagatcggaa
     1561 cggacgtttc tgaacaatca tctctaaaaa agagtccgac gaaggaagtg ttcacacaaa
     1621 tataaaaaca cttttgttca gcaatgattg gttctttgaa tagctataat attaagaaac
     1681 gatcgtgggt ttaagaagat tgtaaggtat tttctatatc gttcacgatc tctggcgaca
     1741 gtggcttctg cctgggatca taccgggcaa tgacagtccc tttacggttg ataaggaatt
     1801 tttggaaatt ccatgtgatc tcacctgtaa agttggtgtc ttcggtgaga tagcgataga
     1861 gagggtgctt ctgttctcca accacagtaa ttttactaaa caacgggaat tccaaagaat
     1921 actttgtata gcaaaattca gcgatttcct tgttgtctcc aggttcctgt tggccaaagt
     1981 catttgcggg aaaggccaaa atcgtaaatc cctgatcgcg gtatcgttca tatagcgttt
     2041 gcagtccggc atattgcggc gtattcccac aaaaacttgc cgtattcacg atcataatga
     2101 cctgtccttt gaactcgttg agtgagcgcg gtttattatc aatatcctcc atgatgaagt
     2161 cgtatatttg tgatgccggc ttactctttg tgatatcaaa tgttgatgac tgttgatttg
     2221 aaggaagtcc ttgaggcgga ggttcattgg caacggcttc gcctaaaggg gtggtatagt
     2281 cctggctaca tccgccaaga ggtactagcc cacacaagat ggctaatatt gcataacgcg
     2341 cagcaaatgg attggggtat ggcatgttag atcctcctca aatgatcgac aatcagtcct
     2401 tctttgactc cagtgtaatc ctcaaattca ggtcagggca attggaccgg atgaaactgg
     2461 caacagtatc accatgtgtt cggacttttt cctaagaaac acgcacaagc ttccagggaa
     2521 agccgaagac atccctgctt gaaaaaacaa tgggagatag gtttaagcag agaatttcat
     2581 tcccgttttc aatagccttg agaattgtca atcgagtggg ctttatagtt atgagacttg
     2641 tcgggacggg ggcagatttt cgtcggagtc tgttcagcat tgttagacca tctatgatcg
     2701 gcatttcgag atcaaggaga attccatcac caggttccgt cttgaggatc tcaaattcat
     2761 acagatcatt cgaatcaatg acccctccgg aatcaaatct ttcctccact gtcgccaatt
     2821 aactaatccc aaaatcttgg tcatcgatca tcagaatccc cttccccatg aattgccaat
     2881 ttaattcatg aatcaatgtt tttctatggt tcgatgccgt taaaccatca tatcgttttt
     2941 agtccatcag gtgtgaaagg aaaaggatga aaccttagac aatatgccgt gaagagaggc
     3001 tggaggataa agtagaggca tgcatgatga aggaaggaag cccttatcgg catgagccag
     3061 gcaaagccca tacaagtgtg aagcctttct gaggtacgtt gttttgtaag aatccgtgaa
     3121 aatttccatt aacctttagc cctggtgtga atcttcaagc caaggaaaga cttgctcgag
     3181 gatcaggtcc ttcttgccga ccccaccaat gaattgcttg aattccccat tcacaaatag
     3241 ggctaatgta ggaagagatt gaatctctaa ttcgccggga atgaggaaat tctcctgcac
     3301 gttcattgcg aggatgagaa ggcgttttcc aaactctttc tggatgtgct ccaattcttt
     3361 ggccagagcg cggcaatgtc cacagcccgg ttgccaaaat tccaccaaaa ccggcacggt
     3421 tgatttttcg attatcccat caaattcgcg gtcttcaatg tttgcaatca tctcattcaa
     3481 ctaagctgat tcttgagcct gataaaatag gaatgcttaa acctaaaaac ggcagttggc
     3541 catctcggaa ctcttcgact gccgcggaag ccgcggcgct cagggcgaac ggactctcac
     3601 ccaacgggtg cccacttcca caccccattc gtgctgaggc tctcgaagca tgaacgggct
     3661 agcagccctg taactgccgt ttttaggcta aagcatgcgt cgcccctgcg atcgggtgac
     3721 gttattccgt gccaattggg tttcgaccat ccgggattcg gttctgacaa gggaacttac
     3781 cttgtccaac gattgtccca tggtaggagg agaaatcgaa tgaagctcca atcatacgct
     3841 tccgggatag ggtggcttca aagccaatac cgcctcttgg aatgttttgc tggacatcgt
     3901 tcccgagttg atacacgagc ccctagtacc cgatgctttt caatgactcc tgctcgcaat
     3961 cccatgttgc ccacttgaac ggcggcccct tgacctgccg gatgtctgcc gatacataaa
     4021 aagtcagctt ctttgataat cgtctgattg aacgagttgg gttgattaag atttgcctaa
     4081 gcaccacctg ttactccaat acaatactgt gctgcacaaa ttttttaata ttggaatcgg
     4141 ttgtaacgat aacaaccgtc caagggtatt tcgtgtcgca aatcgcgttc agaatggact
     4201 ccctccggga agcaaaaatt tcatgaagac ccccatcgac aataaccagt ttcggacggg
     4261 cgatcagaac ccgggccaat aagatttgaa tcgtttgact tggagaaaag ttttttccgc
     4321 cgtattgaac catgccttgt aatccgtcgg gaatgtgttc gatttcctcc tgtaagccca
     4381 ccaaatccaa gacccacaac aaatcttctg tggccagatt ttttcgtctc agggtgatgt
     4441 tttccatcag ggtggattca aagagggtca tatgtcgact caggacgagc ccgcgggttt
     4501 cattgacgtc gtcggggtgg agatcccgga cgtcgatgcc attgtaccgg atggatcctt
     4561 gcggtggatg atccaatccg gccagcagtc tggacaccgt caaccgttgg gtttccgtga
     4621 cacagacgat ggcccatttt tcactcgggg caagttccat gttgagttcc agcgtaggtg
     4681 gccatggtgg aggcaggccc cgtacatggg agatggaaag cgccaaccct tgtggaga
//
LOCUS       DATIAL010000205        10782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1219384, whole genome shotgun sequence.
ACCESSION   DATIAL010000205 DATIAL010000000
VERSION     DATIAL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219384"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VLA60_06445"
     CDS             <1..1147
                     /locus_tag="VLA60_06445"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HSF09034.1"
                     /translation="MPGWVEVEPEQKFGATIPFTTSPTDGTKTVYVWFKDANNNVSVP
                     ASTSILLNTSGYICAAKWGKPGRGASLLHGGEFISPLYGLTIDRQGSLFVVDNGNNRI
                     QKFDRNGNFILLWGNFGSANGGFNNPTGIACDGNGNVYVADTNNHRIQKFDGKLGHYM
                     MKLGSRGNGEGQFNAPWGVAVDRVRGYLYVVDSANFRVQKFDETGEFIMQWGSFGNGD
                     GQFYFARGIAVDQTDGTVYVVDMGNHRIQKFDTSSNVLPQLLTKWGGGIGPGHASSAQ
                     AQEPGQFRSPWGVAVDESGDVFVSDTGNQRIQKFDRDGNFITQWGGFGSQDGQFNFPY
                     GIGADSRGHVYSVDSGNMRVQEFMPAEEAEDHFQEEETMAFIPEAAE"
     gene            1419..2879
                     /gene="guaB"
                     /locus_tag="VLA60_06450"
     CDS             1419..2879
                     /gene="guaB"
                     /locus_tag="VLA60_06450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HSF09035.1"
                     /translation="MLEKHLRMGLTFDDVVLVPRHSNIIPSEVDPSTQLTRNIRINIP
                     ILSAAMDTVTEGRLAIAIAREGGIGIIHRALSPDMQASEVDKVKKSESGMILSPITIS
                     PDHTIRDAHHLMATYRISGIPVTKDGKLVGILTNRDLRFENRLDLKVSQVMTKNNLVT
                     APEGTGLDQAQAILHEHRIEKLPVVNDQFELKGLITIKDIQKKIKYPFACKDAHGRLR
                     VGAAVGVGPDVEERLERLTKAGIDLIVVDTAHGHSQSVLDKVKDIKSRYPDLEIMAGN
                     VATSAATLDLIKAGADAIKVGVGPGSICTTRIVSGAGVPQLTAVADCATIAKEHQIPL
                     IADGGIKYSGDLTKALAAGASCVMIGSLFAGTEESPGETVLYQGRTYKEYRGMGSLGA
                     MERGGKDRYFQEGRESSKLVPEGIEARVPYKGNLAVMVYQLVGGLKAGMGYCGCRTIE
                     ELQQNAQFIQLTSAGLREAHVHDVVITKEAPNYRAD"
     gene            2938..4494
                     /gene="guaA"
                     /locus_tag="VLA60_06455"
     CDS             2938..4494
                     /gene="guaA"
                     /locus_tag="VLA60_06455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HSF09036.1"
                     /translation="MHSHHDTIVILDFGSQYTQLIARRIREFHIFSVILPSQATVQDI
                     LNYHPKGIIFSGGPASVTQAHSPKIDSQIFDLAIPILGICYGMQLLAHQCGGTVNPTP
                     HREFGRADLTIDDNTNLFLGLDPTIAFPVWMSHGDSVQQLPPGFHVIAHTAHAPIAAM
                     RSSNDTQQLFGIQFHPEVIHTLHGSTILQNFARHICHCEPTWTMGSFIEQAVTDIRQT
                     VGNGKVICALSGGVDSTVAAVLAHRAIGDQLTCLFIDNGLMRKNEVAQLQTTFDSAYH
                     LKVRVADHGDRFLSALGRTTDPERKRKIIGRQFIKVFEQEAVSLGKVGFLVQGTLYPD
                     VIESLSVKGPSATIKTHHNVGGLPKRMKFKLIEPFRELFKDEVRQLGLELGLPEDIVW
                     RQPFPGPGLAIRIIGSVTTQRLAILREADAIVRDEIERAGLSRIIWQSFAVLLPIQTV
                     GVMGDQRTYESVIAIRAVTSTDGMTADWGVIPPEVLGKMSNRIINEVQGINRVVYDIS
                     SKPPATIEWE"
     gene            4497..5735
                     /locus_tag="VLA60_06460"
     CDS             4497..5735
                     /locus_tag="VLA60_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSF09037.1"
                     /translation="MAQAEKTVRLQKIIARSGVASRRKAEELIQHGLVTVNGETVMTL
                     GTKVDPAVDHIKVEGRHLKSRPPDMFLMLNKPVGYISTLHDPAGRPTIKALVPKPSIR
                     LFPVGRLDYDSEGLLLLTNNGDIAQACLHPAHHVHKTYLVKIKGILEEPEIQKLRHGL
                     MLEDGPTAPAKVKKAGKAAANSWVEITIHEGRKHQVKRMFEQIGHPVIRLKRVQFGPL
                     NLGTLPPGQTRYLTDKEANDLRHLLIAPESPGPTVRQPKTAQMRLSRPAGQLRPRPTT
                     PNSIRSKNIASPAEKTGQTRPWTPTRPLIKKSEKFPSSRPATPSRPRSPYPNSIKRRS
                     TSPSGGTIGQAGVHRSTDQPIKRRENFPKSGPAGQFWPRQGNPKPIRSKKTISTPGTT
                     GNARPRRPGVTSKSRFKFKT"
     gene            5803..7578
                     /gene="aspS"
                     /locus_tag="VLA60_06465"
     CDS             5803..7578
                     /gene="aspS"
                     /locus_tag="VLA60_06465"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HSF09038.1"
                     /translation="MKRSHHCGELSLDHVGQTVTLAGWVASRRDHGGVRFIDLRDRYG
                     LTQIVFDSEQQPTVHQLADRLRNEYVVAITGEVTLRPEGSANPHIPTGAIEIRSTSLV
                     ILNEAKPLPFALDDTIITTETLRLKHRYLDLRRPKMQELLQLRSQVSHLVRQYLHDNQ
                     FIEVETPILTKSTPEGARDYLVPSRVNPGEFFALPQSPQLFKQILMIGGTDRYYQIAR
                     CFRDEDLRLDRQPEFTQIDLEMSFVDQEDIMQLIEQMVGRVFKETKNIDLPSPLPRLS
                     FEDAMARYGTDKPDLRFDLQLQDLSQFAATCDFKVFKSAVESRGIVKALVIPGGNAFT
                     RNRIDNLIDTAKEFGAKGLAWVKINEEGNLDSSIAKFFNPQALREALPSAKPGDLLIF
                     VADQAPIVLQVLGRLRLLLGEELGLIDRNRWEPLWVIDFPMFEFDDTAKRYIALHHPF
                     TAPHSEDMPLLETDPLQIHTQAYDLVLNGFELGGGSIRIHAPQVQSKVFDLLNISKTE
                     AREKFGFLLDALESGAPPHGGIALGLDRLVMLLGKTESIREVIPFPKTQKAQCLMTQA
                     PSPVTPEQLQELSIRTTAKKPDNPL"
     gene            complement(7575..8606)
                     /locus_tag="VLA60_06470"
     CDS             complement(7575..8606)
                     /locus_tag="VLA60_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF09039.1"
                     /translation="MPFFNNSPFSFPLNRRALFKLGGLAAIGMGFPGCDLGSMFAVPP
                     RETTYFTSNDKFYTVNFMDASYNFTRDLDVEQWKLVVKGAVERPLVMKWRDLLNRESF
                     DQAVTLMCIDTLPGGSSLGTAMWRGISLKKLLQDIGADEDTARDVIFRASDGYSDSIP
                     FARAMEDDVMLAYLMNGEKLPKDHGFPVRLIVPGLYGIKNVKWITEIEVYNGDYLGYW
                     QQKGWTDDGTIHIFSRIDSPGHYQSLQGPQQRLRGIAYGGPESISEVQLSFDQEKTWV
                     SAEIEPPLSPLTWVIWNYDWSPPKPGKHMVSVRAIDTKGKIQTSEITRPQPAGATGLH
                     SIVLDVLNA"
     gene            8707..9723
                     /locus_tag="VLA60_06475"
     CDS             8707..9723
                     /locus_tag="VLA60_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HSF09040.1"
                     /translation="MRIFLTGGAGFLGSHLCETLIHQGHTVICLDNLLTGRTENIGAL
                     MGHPKFSFIHYNVCDYLHVDGPVDAVMHFASPASPQDYLEFPIATMKVGALGTHKALG
                     LAKAKQARFLLASTSEVYGDPLVNPQPESYWGNVNPISPRGVYDEAKRFAEALTTAYH
                     RFHGLDTRIVRIFNTYGPRMRPKDGRVVSNFIVQALQGHPLTVYGDGSQTRSFCYVDD
                     LVAGILSLLQAPPDEFRKNQDVQDAVRFGKTQANLGSIHDPVNIGNPQELTVMDIAKT
                     VIKLTNSSSTITQHPLPIDDPKVRRPNIEKATTLLKWEPRITLEQGLEKAIDYFRSHL
                     DLAK"
     gene            9900..>10782
                     /locus_tag="VLA60_06480"
     CDS             9900..>10782
                     /locus_tag="VLA60_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSF09041.1"
                     /translation="MQNIRQDHILEGILVTLRGSLAACPEVAGAEVMTSTLITNFLHS
                     YPPDKLLTHLGGYGIAAVYDGQHLGPTVMIRCELDGLPVEEADKNQWLVPGANIAHRC
                     GHDGHMAIVAGLAPLLHRTPPPCGRVVLLFQPAEETGAGARAIIDDPNFEQFRPDCAI
                     TLHNLPGFPRGHIVYRRGTFASASVGMRVRLLGQASHAAEPEQARSPANAVGRLLAEL
                     PQLSRVTDNPYCMLTLTHAQLGLMSFGLTPGEATVCATLRAASATGLQNLRREAEQCV
                     RVCAEMERLFAEIEWVQD"
BASE COUNT         2846 a         2884 c         2593 g         2459 t
ORIGIN      
        1 catgcctggg tgggtcgaag tggaaccgga acaaaaattt ggagccacta ttccttttac
       61 cacctcgcca accgatggca caaaaaccgt ctacgtttgg tttaaagacg ccaacaacaa
      121 tgtttctgtt cccgccagca ccagtatcct tctcaacacg tctggatata tttgtgccgc
      181 caagtgggga aaaccaggcc gtggtgcttc ccttctccat ggtggagaat ttattagccc
      241 tctatacggc ctaacgattg atcgccaagg gtcattgttt gtggttgata acgggaacaa
      301 ccgcattcag aaattcgaca gaaatggcaa tttcattctg ttatggggaa atttcggatc
      361 cgctaatggg ggtttcaata atcccacagg catcgcatgc gatgggaatg ggaatgtgta
      421 tgtggctgac accaataacc atcggattca aaagtttgat gggaaattag gccattatat
      481 gatgaaactg ggatcccgcg ggaatggcga aggacagttt aatgctccat ggggagtcgc
      541 cgtcgatcgg gtacgcgggt atctgtatgt cgtagatagt gccaacttcc gggttcaaaa
      601 atttgatgaa accggagaat tcattatgca gtggggaagc tttgggaatg gggacgggca
      661 gttttatttt gcgcgaggca ttgctgtgga tcaaacggat ggaaccgtct atgtggttga
      721 tatgggtaat catcggattc aaaaatttga taccagctca aacgtcctcc cccaattact
      781 taccaaatgg ggcggaggca ttgggcccgg ccatgccagc agcgctcagg cacaagaacc
      841 gggacaattt cgatcgccct ggggtgtggc tgtagatgaa tcaggcgacg tatttgtcag
      901 tgataccggc aaccaacgca tccaaaagtt tgatcgagac ggcaacttta tcacccaatg
      961 gggcggcttt ggttcccagg atggccaatt caacttcccc tatggaattg gagctgactc
     1021 cagaggacac gtctattcgg ttgatagcgg aaatatgcga gttcaagaat tcatgccggc
     1081 agaggaagcc gaagatcatt tccaggaaga ggaaacgatg gcatttattc ctgaggcagc
     1141 agaatagcca caccgccctg gcagaaccca ggaaacaacc acacacacta acaaatgcca
     1201 gagggtatag ggattaccct ttggcatttt tcatagggcc gaaccggatt ccacggtatg
     1261 ggaatacatt tagctgctga acctgagcag tcttgtgtct ctcgataagc ccagcaacta
     1321 taataaaagg cacattgact gcctttcaaa agaaaattaa gatgaaaggc tttttcgcat
     1381 ccacaattcg gcaggggacg acatacaagg cctaactcat gttggaaaaa catttacgaa
     1441 tgggacttac ttttgatgac gtggtcctgg tgccgcgcca ttcaaatatt atcccctctg
     1501 aggttgatcc ctccacccaa ctcactcgaa acattcggat taatatccct attctgagcg
     1561 cggccatgga caccgtaacc gaagggcgct tggccattgc gatagcacgc gaagggggaa
     1621 ttgggataat tcaccgcgcc ctctcccctg acatgcaagc ctcagaagtc gataaggtca
     1681 aaaaatccga aagcgggatg atcctttctc caatcaccat ctccccagat cataccattc
     1741 gcgatgcgca ccatcttatg gcgacctatc gcatatctgg aatccctgta accaaagatg
     1801 gcaaattggt cggcatctta acaaaccgcg atcttcgttt tgaaaaccga ttggatctca
     1861 aagtttctca ggttatgact aaaaacaacc ttgtgactgc ccctgaaggc acgggattag
     1921 accaggccca ggcaattctt catgagcatc gcattgaaaa acttcctgtt gtaaatgatc
     1981 aatttgagtt aaaagggttg atcaccatca aagacattca gaaaaaaatc aaatatccct
     2041 ttgcctgcaa agatgcacac ggacggttac gcgttggtgc ggccgtcggg gtcggacccg
     2101 atgtggaaga acgcctggag cggctcacaa aagccggaat tgatctaatc gttgtagata
     2161 ccgcccatgg tcactcccaa agcgtcctgg acaaggtcaa ggatattaaa tcccgctatc
     2221 ccgacctgga aataatggca ggaaatgtcg caacttctgc ggccacgctg gatctcatta
     2281 aagctggagc agatgctatt aaagttggag tcgggccagg ctcaatttgc accaccagaa
     2341 tcgtatcagg agccggagtc ccgcaattga cggcagttgc ggattgcgcc accatcgcca
     2401 aagagcacca aatacccttg attgctgatg gaggcattaa atattcggga gatctcacca
     2461 aagccctcgc ggccggagcc tcttgcgtga tgatcggatc cctctttgca ggaaccgaag
     2521 aatctccagg ggaaacggtg ttgtatcaag gccgaaccta caaagaatat cgagggatgg
     2581 gatcactggg ggccatggaa cgcggaggga aggatcgcta ctttcaagag ggccgcgaat
     2641 cttcaaaatt agtccctgaa ggcattgagg cccgcgttcc ctacaaagga aacctcgcgg
     2701 tcatggttta tcaattagtc ggagggctga aggcaggaat ggggtactgc gggtgtcgaa
     2761 caatcgaaga acttcaacag aatgcccagt tcattcaact tacctctgca gggctgcgag
     2821 aagcccacgt tcatgatgtg gtgattacta aggaggcccc caattatcgg gctgactaag
     2881 atctgattct gtctccaagt cagtcaggtc ccccgttgac gaattgaccg gcacgccatg
     2941 cactcgcacc acgataccat tgtcattctg gattttggat cgcagtatac gcaacttatt
     3001 gcacggcgga tacgcgaatt ccatattttt tccgtgattc tcccctcgca ggcaacggtt
     3061 caggatatcc ttaactatca ccccaaaggg attattttct cgggaggacc cgccagcgtt
     3121 actcaggccc attctccgaa aattgatagc caaatctttg atcttgccat acccattctg
     3181 gggatctgct acggtatgca attgctcgcc catcaatgtg gcggaaccgt caaccccaca
     3241 cctcatcgtg aattcggtcg agccgatctg accatcgacg acaacacgaa tttatttctt
     3301 ggcctcgatc ccacaattgc atttcctgtc tggatgtccc atggagattc ggttcaacag
     3361 cttccacctg gatttcatgt gatcgcccat acggcccatg ccccaatcgc agcgatgaga
     3421 tcctccaatg acacccaaca gctctttggt atccagtttc accctgaagt catccacacc
     3481 cttcatgggt caacgattct tcaaaacttt gcacgtcata tctgccactg cgagccaacc
     3541 tggacaatgg ggtcattcat tgaacaggcc gtgacagaca tccggcaaac tgtcggaaac
     3601 ggcaaggtca tttgcgcact cagcggagga gtggattcca ctgtggcggc tgttctggcc
     3661 caccgggcaa tcggcgatca attgacctgc ctcttcattg acaatggcct catgcgaaag
     3721 aatgaagtgg cacaattaca aacgacgttc gattccgcct atcatctgaa ggttcgcgtt
     3781 gccgaccacg gagacagatt tctatcagca ctgggacgca caacagaccc ggaacgcaaa
     3841 cgaaaaatca ttggtcgtca attcattaag gtttttgaac aggaagcggt atctttaggg
     3901 aaggtcgggt ttctggttca gggaacactg tatccggatg tgatcgaaag tctgagcgtc
     3961 aagggaccat cggcaacgat caaaacacat cataatgtcg gtggcttgcc gaagcgcatg
     4021 aaattcaaac tgattgagcc ctttcgagag ttgtttaaag atgaagttcg tcaattggga
     4081 ttggaattag gtctaccgga ggatattgtc tggagacaac cctttcccgg tccaggctta
     4141 gcgattcgca tcattggttc cgtgactacc cagcgcctag ccatacttcg tgaggcggat
     4201 gccattgttc gtgacgaaat tgaacgagcc gggctgtcaa gaatcatctg gcaatccttc
     4261 gcggtcttgc tgcctatcca aaccgtggga gtcatgggcg accaacggac ctacgaatca
     4321 gtgatcgcca ttcgtgccgt caccagtaca gacggcatga ctgcggactg gggcgtgatt
     4381 ccaccagagg tgttaggcaa aatgtccaat cggattatca atgaagtaca agggatcaac
     4441 cgtgtggtgt atgacattag ctcaaaaccg ccagcaacga tcgaatggga atagatatgg
     4501 ctcaggcaga gaaaacagtc cgtttacaaa aaatcatcgc ccgttccgga gtcgcttcca
     4561 gacgcaaggc tgaggaactg atccaacatg ggctggtcac cgtcaacggg gaaaccgtca
     4621 tgacgctagg gaccaaagtt gatcctgctg tcgaccacat caaggtcgaa gggcgccatc
     4681 tgaagtcccg gcctcctgat atgtttctca tgcttaataa gccagtggga tacatctcca
     4741 ccttgcatga ccctgcgggt cgtcccacga ttaaagccct ggtcccgaaa ccttccatac
     4801 ggctatttcc ggtcggacgt ttagattatg atagtgaagg cttacttctg ctcaccaata
     4861 acggtgatat tgcacaagcc tgccttcacc ccgcacacca tgtccataaa acatacctgg
     4921 tgaaaattaa gggtatccta gaagaacctg aaatccaaaa actccgacat gggctgatgt
     4981 tggaagatgg gcccacagct ccagccaaag tcaaaaaggc cggaaaagcg gcggctaatt
     5041 cctgggtgga aatcaccatc catgaaggac gtaaacacca ggtcaaacga atgtttgagc
     5101 aaatcggcca tcccgtgatc cggttaaaac gcgttcagtt tggacctctg aatcttggaa
     5161 ccctcccccc aggccagacg cgatatttaa cagataagga agcgaacgac ttacgccatt
     5221 tactcatcgc acctgaatca ccaggtccga ctgtccgcca gccgaaaact gcacagatgc
     5281 gcctatcgag acctgcaggt caattacgac cacgccccac aacccccaat tccatcagaa
     5341 gcaagaatat tgcgtcacct gccgaaaaaa ccggccaaac acgaccatgg acaccaactc
     5401 gtccgctgat taagaaatcg gaaaaattcc cttcctccag acctgcgact ccttcgcggc
     5461 cacgctcacc atatccgaat tccatcaaaa ggaggagcac ttcaccgtct gggggaacga
     5521 tcggccaagc aggggtgcac cgatccacag atcaaccgat caaaagaagg gaaaattttc
     5581 ctaaatcggg acctgctggg cagttttggc cacgccaggg aaacccgaaa cccatcagga
     5641 gtaagaagac gatctcaact cctggcacaa ccggcaatgc acggccccgc cgacccggcg
     5701 tcacatcaaa aagccggttc aagtttaaaa cttaagcatt ccgtattctc ggttaaacgt
     5761 tctgccatca aaagctgtta ggcgttggaa aggatttttt cgttgaaacg atcacatcat
     5821 tgcggtgaac tctccttaga ccatgtaggc caaaccgtga ccttagccgg ctgggttgcc
     5881 agtcgccgcg accatggcgg agtgaggttc attgatcttc gtgatcgcta cggcctcaca
     5941 caaatcgtgt ttgattcaga gcagcaaccc actgtgcatc aactagccga tcgtctccgc
     6001 aatgaatacg tcgtggcaat caccggtgag gtgacgttac gtcccgaggg ctcagcaaac
     6061 cctcatattc cgactggcgc catagaaatt cgaagcacat ccctcgtgat tctcaatgag
     6121 gccaaacccc tcccctttgc acttgacgac accatcatca ccacagagac acttcggtta
     6181 aaacaccggt atctggacct tcgccggcca aagatgcaag aactccttca actccgcagt
     6241 caggtgtccc acctcgtacg gcaatacctt catgacaatc agttcataga agtggaaaca
     6301 ccgattttaa ctaaaagtac tccggaaggc gcacgagatt atcttgtccc gagtcgagtc
     6361 aacccgggtg aattttttgc tcttccccaa tcaccgcaac tctttaagca gattctgatg
     6421 atcgggggaa ctgatcgtta ttatcaaatt gcccgatgct ttcgtgatga agacctccgg
     6481 ttggatcgtc agccagaatt cacgcaaatc gatctggaaa tgtctttcgt cgatcaggaa
     6541 gatatcatgc agttgataga acagatggtc ggacgggtct ttaaagagac gaagaatatt
     6601 gacctcccct ccccattgcc ccggctgtcc tttgaggatg ccatggcacg ctatgggaca
     6661 gacaaaccgg atctccgctt tgatcttcag ttacaggacc tgagtcaatt cgccgccacc
     6721 tgcgacttta aagtttttaa gagcgcagtg gaaagccgcg ggatcgtaaa agccctcgtc
     6781 attcccggtg gcaacgcctt tactcgcaac cgaattgaca atcttattga taccgcaaaa
     6841 gaatttggtg ccaaggggct agcctgggtc aaaattaacg aagagggcaa tctggattcc
     6901 tctatcgcca aattctttaa tccccaagcc ctaagggaag cgctcccctc cgccaaaccc
     6961 ggtgacctgc tcatatttgt cgcagatcaa gcgccgatcg ttcttcaggt cttaggccgc
     7021 cttcgcctgc tgctggggga ggagctagga ttgattgatc gtaaccggtg ggaaccgctg
     7081 tgggtcatag actttcccat gtttgaattt gacgataccg ccaaacgata tatcgctcta
     7141 caccatccct ttacggcccc tcactcagaa gatatgccgc ttctggaaac cgatcctctt
     7201 cagattcaca cgcaagccta tgacctggtt ttgaatggct ttgagttagg aggaggaagt
     7261 attcgaattc atgcgccaca agttcagtca aaagtctttg acttactcaa catctccaaa
     7321 acggaggctc gtgaaaaatt tggatttctc ttagatgcct tggaatcagg ggcaccgccc
     7381 catggaggca tcgcgctggg acttgatcgg ttggtgatgt tactcggtaa gacggaatcg
     7441 attcgtgaag tgattccgtt tcctaaaaca cagaaggctc aatgcctgat gacccaagcc
     7501 ccctcccccg tcacaccaga acaactacag gagctctcta ttcgcacgac ggctaaaaag
     7561 cctgacaacc cgctttaagc gttcagcaca tccagaacaa tggaatgcaa tcccgtcgcc
     7621 ccagccggtt gtggtctcgt gatttccgag gtttggatct ttcccttcgt atcgatagcc
     7681 ctgacagaca ccatgtgctt gccaggtttt ggcggactcc aatcataatt ccaaatcacc
     7741 caggttaaag gagacaacgg tggttcaatc tcggcagaca cccaggtttt ctcctgatca
     7801 aaactcagtt gaacctcact gatgctctcc ggtcccccat aggcaatccc gcgcaaccgc
     7861 tgttgaggcc cttgtaaaga ctggtaatga cccgggctat cgatgcgaga aaaaatatga
     7921 atggtcccat catccgtcca acctttttgt tgccaatagc ccaggtagtc accgttatag
     7981 acttcgattt ccgtaatcca cttcacattt ttaatcccgt agagacctgg aacaatcaga
     8041 cgaaccggaa agccatgatc cttaggaagt ttttccccat tcatcagata ggccaacatg
     8101 acatcatctt ccatcgccct ggcaaatgga atactgtcgg aatacccgtc agatgctcga
     8161 aaaatcacat cccgggcggt gtcttcatca gctccgatgt cttgcaaaag ttttttaagg
     8221 gagatccctc tccacatagc ggtccccaaa ctactcccgc ccggaagggt atcaatacac
     8281 atcagcgtca cagcctgatc aaaggattcc cgattgagca gatcacgcca tttcatcacc
     8341 agagggcgtt ccacagcgcc tttaaccacc agtttccatt gctccacatc caagtcgcgg
     8401 gtaaaattat aggaagcatc catgaaattt acggtgtaaa atttatcgtt tgacgtaaaa
     8461 tatgtggtct cccggggagg aaccgcaaac atcgacccca gatcgcatcc aggaaatccc
     8521 atgccgatgg cagccagtcc cccaagcttg aaaagggctc gacgattcag tggaaaagaa
     8581 aaaggagaat tattgaaaaa tggcatagac atcctctcta ttatctaata cctttacgac
     8641 tttggacaag atccacgcga catgtgaaat taccacggaa tttatgcgaa gaggaggaga
     8701 aacggcatgc ggatattctt gacgggaggg gccggttttt taggaagtca tttatgtgaa
     8761 acgcttatcc atcaaggaca tacagtcata tgcctggaca atctccttac cggacgtaca
     8821 gagaatatcg gtgcactcat gggacatccg aagttctcgt ttattcacta caatgtctgc
     8881 gattatcttc atgtcgacgg accagtcgac gccgtcatgc attttgcatc accggcgagc
     8941 ccccaggact atctggaatt tcccatcgcc accatgaaag tcggcgcgtt aggcacacac
     9001 aaagcgttgg gcttagccaa ggcgaaacag gcgcgatttc tcctggccag cacatccgaa
     9061 gtctacgggg atccactggt caacccacag ccggaatcct attgggggaa cgtcaacccc
     9121 attagcccca gaggcgtcta cgacgaagcg aaacgatttg ccgaagcctt gaccaccgcc
     9181 taccatcgct ttcacggcct cgatacaaga atcgtcagaa tcttcaacac ctatggccca
     9241 cggatgcgcc caaaggatgg gcgggtggtc tccaacttta tcgtgcaagc cttacaaggg
     9301 catccgttaa cggtctatgg cgatgggtca cagacccgca gcttttgtta cgtggatgat
     9361 ctggtcgcgg gcatcctatc gcttctccag gccccgccag acgaattccg aaaaaatcaa
     9421 gatgttcaag atgcggttcg cttcggaaaa actcaggcca acctcggctc catacatgac
     9481 cctgtaaaca tcggcaatcc acaagaactc accgtcatgg acattgccaa aacagtgatc
     9541 aaactcacca actcatcgag cacgattacg caacacccgc ttccaatcga tgaccctaaa
     9601 gtacgccgac ccaatatcga aaaggccacc actctgctca aatgggaacc tcggattact
     9661 ttggaacaag gccttgaaaa agccatcgac tatttccgtt ctcatttgga ccttgcaaag
     9721 tgaatcacgg atcatgagtt tcatcgattc cgtgcaatct gtgacccatc acataccctg
     9781 tcctgatcgt attcggatac aacaccatag gctcgacaag tccgcatctg gccgaaagct
     9841 ccgaacgcaa ccgatctacg cgggtggaca gagacaccct atcaccatag aataaaaaca
     9901 tgcagaacat tcggcaggac cacatacttg aaggcatttt agtcaccctt cgtggctctc
     9961 tcgcggcttg tccggaagta gccggggcag aggtcatgac gtcaacactc atcacgaatt
    10021 tcttacactc ctatccaccg gacaaactgt tgacccatct cggtggttat gggatcgcag
    10081 cggtctatga tggccaacat cttgggccaa cggtgatgat acggtgtgaa ctggatggac
    10141 tgccggtgga agaagcagac aagaaccaat ggctcgtacc aggagcgaac atcgcccacc
    10201 gatgtgggca tgacggacat atggccatcg tggcgggctt ggcccccctg cttcatcgca
    10261 ccccaccacc ctgtggacga gtggtgctct tgtttcagcc cgcggaggaa acaggggcag
    10321 gcgcacgggc aattatcgat gacccgaatt ttgagcagtt ccgtccggac tgcgccatca
    10381 ccctgcataa tctcccaggt tttcccaggg ggcatattgt gtatcgacgg gggacgtttg
    10441 ccagtgcctc tgtggggatg agagttcgtc tccttggtca agcgtctcac gcggccgagc
    10501 cggaacaagc gcgatctcct gcgaatgcag taggacggtt actggctgag cttccccaat
    10561 tgagcagggt cacggacaac ccctattgta tgcttacgct aacccatgcg cagttggggc
    10621 tcatgtcgtt tgggctcaca ccgggagaag caaccgtgtg tgccacgctt cgagcagctt
    10681 ctgcgacggg ccttcaaaac ttacgacgcg aagccgaaca gtgtgttcgc gtttgtgccg
    10741 agatggaacg gctatttgca gaaatcgagt gggtgcagga tt
//
LOCUS       DATIAL010000206         6090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_144643, whole genome shotgun sequence.
ACCESSION   DATIAL010000206 DATIAL010000000
VERSION     DATIAL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144643"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1308)
                     /gene="proB"
                     /locus_tag="VLA60_06485"
     CDS             complement(172..1308)
                     /gene="proB"
                     /locus_tag="VLA60_06485"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HSF09042.1"
                     /translation="MREQVLAACKRIVVKVGSSLVASSQGGLRLDRINRLAQELGTLQ
                     AQGRQIVVVSSGAIVAGISHLAILSYPTELPLQQAAAAVGQSRLMRAYEMAFEETKNK
                     IAQVLLTHQDLSDRRRFLNARHTLNALIQLRVIPIINENDTVAIEEIRFGDNDTLAGE
                     VAHLVDADLLVILSDVDGLYSQDPHLNPSAELIPLVANVTKDIEDLAGTSRTQASRGG
                     MVTKIKAAKQAGRFGVPTLLLNGETPNALGEVLNGNPGGTFFVSDQSPLTSRKQWIAY
                     TLRPKGELRLDEGAVAALTLRGKSLLPSGILDITGIFLTGDPITCATREGMPFAQGLT
                     NYSAETLHRIKGKKTSDIRQLLGSLEYEEVIHRDNLVLTKHQVS"
     gene            complement(1290..2309)
                     /gene="obgE"
                     /locus_tag="VLA60_06490"
     CDS             complement(1290..2309)
                     /gene="obgE"
                     /locus_tag="VLA60_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HSF09043.1"
                     /translation="MFIDQARIFIKAGDGGTGHCSFRREKFAEFGGPNGGDGGDGGDV
                     VFVASNRVSTLLDLRYQKHYEATSGVRGLKTNCHGANGSDIVIPLPVGTLVKTEDGEI
                     IADLIAEGQTAIVAKGGKGGKGNARFATSTNRAPRQFEPGTPGEERWVNLELKLLADV
                     GLVGFPNAGKSTLISAISSAHPEIASYPFTTLRPHLGVVEWMEHSSFVVADIPGLIEG
                     AHEGKGLGIKFLRHIQRTAFLLYLVDITEWIAEDPVEIVETLQRELKAFDPELLERPF
                     AIVATKIDSQGNGQHLEALREYCRAHHYPFFPISAVTREGLPSLVTYLGKAVLEAKTS
                     CASKC"
     gene            complement(2392..2646)
                     /gene="rpmA"
                     /locus_tag="VLA60_06495"
     CDS             complement(2392..2646)
                     /gene="rpmA"
                     /locus_tag="VLA60_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HSF09044.1"
                     /translation="MAHKKGGGSSRNGRDSNPQYLGVKAYAGEKVSGGSIIVRQRGTK
                     FFPGANVGLGSDYTLFAKIDGIVKFEGGIARRKVSVYPPV"
     gene            complement(2685..3002)
                     /gene="rplU"
                     /locus_tag="VLA60_06500"
     CDS             complement(2685..3002)
                     /gene="rplU"
                     /locus_tag="VLA60_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HSF09045.1"
                     /translation="MYAIIETGGKQYRAEPNGILQVESLEGDVGSIIQFDSVRFVQTD
                     QAPIVGSPMITDAVVKGEIIRHARTRSITIFKKKRRKNYRRTKGHRQGFTQVRITEIV
                     ASA"
     gene            3168..4082
                     /locus_tag="VLA60_06505"
     CDS             3168..4082
                     /locus_tag="VLA60_06505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HSF09046.1"
                     /translation="MVTVSKKIWMDGALVDWDEASVHVLTHSLHYGLAAFEGIRCYEG
                     KAGSNIFRLQEHVDRLFESAHITMMPMPFTKKEVSDAIVETVRVNQLASCYIRPIAYV
                     GYGAMGVYPGDNPIRLAIAAWPWGSYLGEDALAQGIRAKISSFTRHHVNVSMTRAKIS
                     GYYVNSILAKWEAKKSGYAECILLDPEGYVAEGTGENVFIVKKGVLKTTPLTSILDGI
                     TRNSILELARARNIPVIEERFTRDAMYVADEIFLTGTAAEVTPVRELDDRKIGEGKPG
                     ALTKSLQDDFFRIVRGEEAAYANWLTPI"
     gene            complement(4169..4540)
                     /gene="secG"
                     /locus_tag="VLA60_06510"
     CDS             complement(4169..4540)
                     /gene="secG"
                     /locus_tag="VLA60_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HSF09047.1"
                     /translation="MYTLTVILHIIVCFLMIAAILLQAGKGAEIGASFGGSSQTVFGS
                     RGPGTFLSKITVGAAIVFMLTSLSLALLSKQANTSSTVIDLHPTSHHETSSPATPETT
                     PPTETGTAEPASETTPAPTAP"
     gene            complement(4572..5330)
                     /gene="tpiA"
                     /locus_tag="VLA60_06515"
     CDS             complement(4572..5330)
                     /gene="tpiA"
                     /locus_tag="VLA60_06515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HSF09048.1"
                     /translation="MRRRFIVGNWKMHKTISQAESLVTDILQIYQPHASVEMAIAPPF
                     VSLPAVARLLASTSIKLAAQNTCASDEGAFTGEISPPMLRDVGCHYVIIGHSERRHIF
                     GETDQDINKKVHAALHHDLLPILCVGERLEERQSNKTQTVIQQQLQACLEGLESKDFA
                     KITIAYEPVWAIGTGQAATVDQATEVHGHIRSFLAMQWGIKPDQTRIIYGGSVTQDNA
                     KSLFQSSQINGALVGKACLNSESFVKIAELASSN"
     gene            complement(5376..>6090)
                     /gene="pgk"
                     /locus_tag="VLA60_06520"
     CDS             complement(5376..>6090)
                     /gene="pgk"
                     /locus_tag="VLA60_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSF09049.1"
                     /translation="ITKYVKVSAAGFLMKREVEALEGTVENPVRPFVAILGGAKVSGK
                     IGVIENLGKVVDKVIIGGGMAFTFIKAMGLEIGQSLVEKDMLDFAKRIHEQAIAQKIK
                     FYLPVDCVVAASLDPEAETKIVPVQEIPAGWYGMDIGPASVRLFSEAVQNAKTILWNG
                     PMGVFERDAFSRGTYAMAHAVANAYAKTIVGGGDTALAVYRAGESESMSFISTGGGAA
                     LQLLEGKHMPGLAALPDRL"
BASE COUNT         1431 a         1459 c         1604 g         1596 t
ORIGIN      
        1 ggtctttgtg aacactccac catatttctc tgatgaggct cggtcattca tatgctgagg
       61 aaacggcaat atttttgaaa tattcgacgg ctccctgtgt aattctagga gataaaggcg
      121 atctatacct gtgccaatgg agcattgtgc atgagccggg agccacgttg cttatgagac
      181 ctggtgcttt gtcagaacca gattgtcccg gtgaatgact tcctcatact ccaatgagcc
      241 caggagttgt ctaatgtctg acgttttttt ccccttgatc cggtggaggg tttcggcaga
      301 ataattcgtg agtccctgcg cgaacggcat tccctcccgg gtggcgcagg tgatggggtc
      361 tcctgtcaga aagatgcctg tgatgtccag aatgcctgag ggtaataagc tttttcctcg
      421 aagggtgagg gcggccaccg ctccttcatc taaccggagc tccccttttg gtcgaagcgt
      481 gtaggcaatc cactgttttc gactggtcag cggtgattgg tctgacacaa aaaacgttcc
      541 cccgggattt ccgttcagga cctctcctaa ggcatttggc gtctctccat ttaataaaag
      601 ggtcgggact ccaaagcgtc ctgcctgttt ggcggctttg atcttggtga ccatgcctcc
      661 acggctggct tgtgttcggg aggttccggc gaggtcttca atgtccttgg tgacgttggc
      721 aaccagggga attaattctg ccgaaggatt gaggtgagga tcctggctat agagcccatc
      781 gacatctgat aaaatcacca gcagatctgc gtcaaccaag tgggccactt cccccgcgag
      841 ggtatcattg tcaccaaatc tgatttcctc gatagccacg gtatcatttt cattaataat
      901 tggaataacc ctcagttgaa taagggcatt taaggtatgg cgggcattca ggaatcgtcg
      961 acggtcggat aaatcctggt gagttaagag gacctgggca atcttgtttt tcgtttcttc
     1021 aaacgccatc tcataagctc gcatgagccg gctttgtccg acggcagcgg cggcttgctg
     1081 aaggggtaat tcagtgggat aggaaagaat ggcgaggtgt gagatgcccg caacgatggc
     1141 tcccgaggaa accacgacaa tttgtcgacc ctgagcctgc agggtgccca attcctgggc
     1201 taaacggttg atgcggtcga gtcgtagacc gccttgcgag gaagctacca ggctgcttcc
     1261 cactttgacg acaatcctct tgcacgctgc tagcacttgc tcgcgcatga ggtctttgct
     1321 tccagaacgg ctttccctaa ataggttacg agtgatggaa ggccttccct ggtaacagcc
     1381 gaaatgggaa agaatggata gtggtgtgcg cggcaatact cccgaagggc ttccaagtgc
     1441 tggccgtttc cctgagaatc aatttttgtg gccacgatgg caaatggccg ttcaaggagt
     1501 tcggggtcaa aggctttgag ttccctttgt aaggtttcaa cgatctcgac gggatcttct
     1561 gcaatccatt cggtgatatc gaccagatat aacaaaaaag cggttcgctg gatatgccgc
     1621 aggaatttaa ttcctaaccc tttcccctca tgagcgcctt cgatcaatcc tggaatgtcg
     1681 gctacgacaa atgacgagtg ttccatccat tccactactc ccaaatgagg ccgcagggtg
     1741 gtgaagggat aactcgcaat ttccgggtgt gcagaagaaa tcgctgaaat caaagtggat
     1801 tttccggcgt taggaaagcc caccaagccg acatcggcta acagtttcaa ttccagattc
     1861 acccatcgct cttcaccggg agtccctggc tcgaattgac gcggtgctcg attcgttgac
     1921 gtggcaaaac gtgcgttgcc ctttccgcct ttgcctcctt tggccacaat ggcggtttgt
     1981 ccttctgcaa taaggtcggc gatgatttcc ccgtcttcgg tttttacgag agttccgaca
     2041 ggaaggggaa tcacaatatc ggagccattg gctccatggc agttggtctt gaggcctctg
     2101 acgccggaag tggcctcata atgtttttga tatcggaggt caagtaaggt tgagactcta
     2161 ttggaggcca caaagactac gtctcctcca tcaccgccgt cgccaccgtt gggaccgcca
     2221 aattcggcaa atttttctcg tcgaaagctg cagtgtccgg ttccaccgtc acctgctttg
     2281 atgaatattc gtgcttgatc gataaacata ctgatcctta agaaatccta ccggacttac
     2341 cagggaaaaa tgaaggagat gaattgataa agcaggaaat gggaagggga gctagacggg
     2401 gggatagacg ctcaccttcc ggcgggcgat tcctccttca aacttaacga tcccatctat
     2461 ttttgcaaag agggtgtagt cgcttccaag tccgacattt gccccaggaa agaattttgt
     2521 tccgcgttga cgaacgatga tgcttccgcc gctcaccttt tcgccggcat aggctttgac
     2581 gcctaaatat tgtggattgc tatcgcgacc gttgcgggat gatccgccac cttttttatg
     2641 tgccatgggt caaacctcca aaaaattcta aagaccaata tttattaagc agatgccaca
     2701 atttcagtga ttcgaacttg cgtgaatccc tgacgatggc ccttggtccg tcgatagttt
     2761 tttcgtcgtt tctttttgaa aatggtaatg gaccgggttc gcgcatgtcg gataatctct
     2821 cctttgacta ccgcatcagt gatcatggga gagccgacaa taggcgcttg gtccgtttgg
     2881 acaaatcgca ctgagtcgaa ttgaatgatt gacccgacat cgccttcaag tgactcgact
     2941 tgtaggatac cgtttggttc cgcccggtat tgttttccgc ctgtttcgat aattgcatac
     3001 atgtttttta gctccttaac aaacacgcat tttgccagta tgtttacatt tatgtcaaga
     3061 ttttagtata tcatcacgcg ttcttaatgc ttagcaaaaa aacacgtgat acccttcgct
     3121 cacatgattt gtctatttca gcttctgtca atcggagaag tatttctatg gtgactgtgt
     3181 caaaaaaaat ctggatggat ggagccctcg tcgattggga tgaggcctcg gttcatgttc
     3241 ttacccattc tctccattat ggtctggcgg cctttgaggg gatccgttgt tatgagggga
     3301 aggccggatc aaacattttt cgattacaag agcatgtgga ccggctcttt gagtctgccc
     3361 atatcacgat gatgcccatg ccttttacca aaaaagaggt gtcggacgct attgtcgaga
     3421 cggttcgggt caatcaattg gcctcctgtt atatccgccc gatcgcctat gtgggatacg
     3481 gcgcaatggg agtgtaccct ggcgacaacc cgattcgtct ggctattgcg gcttggccat
     3541 ggggatcgta tttaggggag gacgccttgg cgcaaggcat cagggcaaaa atttcatcct
     3601 tcacccggca ccatgtgaat gtatccatga cccgggcaaa aatctcgggc tattacgtca
     3661 attcgatttt agccaaatgg gaggcgaaga agtccggata tgccgaatgt attcttcttg
     3721 atcctgaagg atatgtcgct gagggaacag gagaaaacgt gtttattgtg aaaaaagggg
     3781 tcttaaaaac gacgccattg acctcgatcc tggacgggat taccagaaat tcgattcttg
     3841 aattggcgag agccagaaac atcccggtga ttgaggagcg gtttacccgg gatgcgatgt
     3901 atgtggcaga cgaaatattt cttaccggaa ccgctgccga agtgactccg gttcgcgaat
     3961 tggatgatcg aaaaatcgga gaaggtaagc caggggctct cacaaaatcc ctgcaggatg
     4021 atttctttag aattgtgcgt ggggaagaag ctgcgtatgc caattggctg actcctattt
     4081 aggagttgcc cttctaatta ggtggcccca cgatcctggg ctattccacc aattgtcaat
     4141 atagttgcgg actcagtaaa aaaatagttt atggagcagt cggggcggga gtcgtctctg
     4201 acgcgggttc agctgtgccg gtttcagttg ggggagtggt ctcgggtgtc gccggagagg
     4261 acgtttcatg gtgtgaggtt gggtggagat ctatcacggt ggacgaggta ttggcttgtt
     4321 tcgaaagtag tgccaggctg agcgatgtca gcataaacac aatagcggct cccaccgtga
     4381 tcttacttag aaacgtaccg gggcctcggc ttccaaacac cgtttgagaa gaccctccaa
     4441 acgaagcacc gatctcggcg ccttttcctg cctggagcaa aatggcggca atcatgagaa
     4501 aacaaacaat gatatgaaga atgacggtca aggtatacat aatgtgtgcg tgtttgttgg
     4561 agtgttcctg gttaattgga agaagctagt tcggctatct taacaaagga ttcggaattc
     4621 aaacaagctt ttcccacgag ggccccatta atttgtgaag attgaaagag acttttggca
     4681 ttgtcctggg tcacgctccc accgtaaatg attctggttt gatcaggttt gatgccccat
     4741 tgcatggcaa gaaaagaccg gatgtggcca tggacctcgg ttgcctggtc gacggtagcc
     4801 gcctgtccgg tcccaatggc ccagacaggt tcgtaggcga tggtgatctt tgcaaaatct
     4861 ttggattcga gaccttcaag gcaggcttgc aattgctgtt gaatgacggt ctgcgtttta
     4921 ttggattgac gttcttccag acgttccccg acacataaaa tcggtaggag gtcatggtgg
     4981 agggcggcgt ggactttttt gttaatatcc tggtcggttt ctccaaaaat atgccgtcgt
     5041 tcggaatgcc caataatgac atagtgacag ccgacgtccc gtagcatggg tggcgaaatt
     5101 tctccggtaa aggcgccctc gtcactcgca caggtatttt gagcagccaa tttgatggaa
     5161 gtcgaagcta gcaagcgtgc aactgctggc aatgatacaa aggggggagc gatggccatt
     5221 tctaccgaag cgtgcggttg atatatctgg agaatatcgg tcaccaagga ctcggcctgg
     5281 gaaatcgtct tgtgcatttt ccagttgccg acaatgaatc gacgacgcat gaggacctct
     5341 taacttttgg aattcaaaaa aggggggcgt tgggatcaaa gacgatcggg taaggctgca
     5401 agccccggca tatgcttgcc ttccaggagt tgaagggccg caccaccacc ggttgaaatg
     5461 aacgacatac tttccgattc tccggctcga tacacggcga gagccgtatc tcctcctccc
     5521 acaattgttt tcgcataggc attggccaca gcatgggcca ttgcataggt cccgcgtgaa
     5581 aatgcatcac gctcaaagac tcccatgggg ccattccaca aaatggtttt ggcgttttgg
     5641 acggcttcac taaacaggcg gaccgacgcg ggcccgatgt ccatgccata ccagcccgcg
     5701 gggatttcct gcaccgggac aatttttgtt tctgcttcag ggtcgagact ggccgcgacg
     5761 acacaatcga cgggaagata gaatttgatt ttttgagcta tcgcctgttc gtgaatgcgt
     5821 ttggcaaagt ccagcatgtc tttttcgacg agagattggc cgatctccaa tcccatggcc
     5881 ttgataaagg taaaggccat gccgccaccg atgatgactt tatcgaccac cttgccaaga
     5941 ttttcaatca ccccgatttt tccggaaact tttgcccctc ccaaaatggc cacaaacgga
     6001 cgaacagggt tctccaccgt gccttccagg gcttcgactt cccgtttcat cagaaatcct
     6061 gccgccgata ctttgacata tttggtgatg
//
LOCUS       DATIAL010000207         6544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_897229, whole genome shotgun sequence.
ACCESSION   DATIAL010000207 DATIAL010000000
VERSION     DATIAL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897229"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VLA60_06525"
     CDS             <1..906
                     /locus_tag="VLA60_06525"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HSF09050.1"
                     /translation="ITAPLEDDVTPVTFVIGGDTRHSFQPFSIMETMRTVSPDFFVFL
                     GDTIYADKDTPARELSDYWQKYRENRDGFARRLLAETSVYAMWDDHEVDSDFTSTNPL
                     LPIGRQAFFTYWPIRPYTPDPTRLYRTFRWGKAVELFLLDTRQYRNPATNTMLGTDQK
                     QWLLAQLQASSARFKFIISSVPFSDPRVDKWGEYPEERDEILQFLDKMGITGVLFLAG
                     DVHLGAVSRMPGLTDRKEFIFGPLAAQMNYKISNREPRFEYFNDASRNFGKITVDPNG
                     LKPSVHIEWFDANKTLLHQVILEEN"
     gene            1202..1336
                     /locus_tag="VLA60_06530"
     CDS             1202..1336
                     /locus_tag="VLA60_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09051.1"
                     /translation="MITQHRLLTRIARWVDAGKIQSTLTEPLSPLNTAHLRTAHTKIE
                     "
     gene            1528..2355
                     /locus_tag="VLA60_06535"
     CDS             1528..2355
                     /locus_tag="VLA60_06535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF09052.1"
                     /translation="MMPIPSHSSSLKNPPAWLCELPKKDPWSRPFAESLLTHLDLFEG
                     ATILDVACGDGIPAFYLAHHVGPGGQVVGIDANHAQLIRARAVQGPYFPWLEFRLGDV
                     RTLPPDLGQFDRITGNLSFMFFRPHRLEALQQLARFLKPGGQLVLTFPSLGTFDSLWK
                     RVDREMTQQGLTKEREAFADYLAERPSAVDARTWLAACGLINIDVMVYPLEVHTGPGQ
                     EFLYHPLLRGGFLDDVYECFDDQTLADQFMDTISLDTQSFVPLFAQRCVMAGWLPEK"
     gene            2559..3569
                     /locus_tag="VLA60_06540"
     CDS             2559..3569
                     /locus_tag="VLA60_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSF09053.1"
                     /translation="MAEEVTPDRIMQLGMAFWGSKTLLSAVELGVFSELAKQPLDAQA
                     LQARLGLHPRSARDFFDALVALGMLDRQNGHYANTSETDLFLDRAKPTYLGGWLEMVN
                     FRLYRFWGKLTEGLRTGLPQNEVKEGKNFFEELYSDPRRLKEFLSSMTGLSMGASRKI
                     ASQFPWKQYHTFIDVGSAQGGLAVQVGLAHEHITGGGFDLPVTGPVFKEYVQSFGLSS
                     RLKFHPGNFFSDPLPKAEVLSMGHILHDWNLEEKKMLIAKAYDALPPGGALIIFESLI
                     DDERRSNAFGLLMSLNMLIELPGGFDYTGADCTGWLREAGFRETRVEHLIGADSMVVG
                     IK"
     gene            3993..4988
                     /locus_tag="VLA60_06545"
     CDS             3993..4988
                     /locus_tag="VLA60_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSF09054.1"
                     /translation="MNTSRWVFMAGTLIIVFAGGWYGMIWMPGSSYQGALPVATDEIR
                     DLEQKLHTHVHTLSTQIGERNLFHYDKLLLAAEYIRAEFKAAGYDALSQKFDVSGNIC
                     ENIEAEVRGTNRREDILVIGAHYDSVKGSPGANDNGTGIAAMLALARQFAQSPVSRTL
                     RFVAFANEEPPFFQTPSMGSWVYAQRSRQQNERIVLMLSLETIGYYTEAPSSQRYPPP
                     LSLIYPSTGNFIGFVSNLANRDWVQRAVGAFRQHTQFPSEGAALFEWLPGVGWSDHWA
                     FWQEGFPALMVTDTALFRDPNYHTTKDTPDHIHFGHLARVVTGLEHVIRDLANQP"
     gene            complement(4995..6443)
                     /locus_tag="VLA60_06550"
     CDS             complement(4995..6443)
                     /locus_tag="VLA60_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09055.1"
                     /translation="MIRIFLLCVVGLLSLIGCSAFQTVPDLGNLYNKLAQQEDPLRNP
                     VIVIPGILGSRLEEGNTREVVWGAFGSGAVDPTTAEGARLLALPMAPGVALKDLRDTV
                     RQAGVLDRVVLKFLGIPLELNAYYNILRTLGVGGYRDQQLAESHAVEYGNRHFTCFQY
                     AYDWRRDLVESAQGLDRFIKAKEAEVQEETFRRFGMKRSPVKFDLVAHSMGSLVARYY
                     LRFGAADLPADGSLPPVTWEGARHVDHLVVIGPPNAGSVETLTILVDGLKPAPLLSTY
                     PSAIVGTMPSVYQLLPRSRHQPLFDVNDQPVTNVFDPTLWERQGWGLADPSQDRILKI
                     LLPDIADPQERRRVALDHQRKALDRAKQLTSALDVHATPPPTTRFLLVAGDSMSTMSA
                     AQIQDDGRLKITKHAPGDGVVLRRSALMDERNEKMPGSRLISPIPWSQVLFIFSDHLG
                     MTRDPAFVDNILYFLLESPRGESIGSKTGPSS"
BASE COUNT         1581 a         1786 c         1662 g         1515 t
ORIGIN      
        1 atcacggctc cacttgaaga cgacgtgacg cccgtgacct ttgtgattgg aggtgataca
       61 cgccatagtt tccaaccttt ttcaattatg gaaaccatgc gcacggtgag tccagacttt
      121 tttgtctttt tgggagacac tatttacgct gacaaagaca cgcctgcccg agaattatca
      181 gactattggc aaaagtatcg agaaaaccgt gacggatttg cccgacggct tctcgccgaa
      241 accagtgtct atgccatgtg ggacgatcac gaagtggaca gtgacttcac ctcaacgaat
      301 ccactcctgc ctattggtcg ccaggccttt tttacctact ggcccattcg cccatacacg
      361 ccggatccca cccgtctcta ccggacattt cgctggggga aagccgtgga actttttctc
      421 ctggataccc gccaatatcg gaacccggca accaacacga tgttgggaac tgatcaaaaa
      481 caatggctgt tggcccagct ccaggcctct tccgccagat tcaaatttat tatctcctcc
      541 gtcccctttt ccgatcctcg ggtcgacaaa tggggagaat atcccgagga gcgagatgag
      601 atcttgcagt ttttagacaa aatgggcatt accggcgtgc tgtttcttgc cggtgatgtg
      661 catcttggag cggtgagccg catgcctggg ttgacggatc gcaaagaatt tattttcggt
      721 ccgctggcgg ctcagatgaa ttacaagatt tctaatcgag agccacgatt cgagtacttt
      781 aacgacgcct cacgaaattt tgggaaaatc accgttgatc ctaatggctt gaagccgtcg
      841 gtccacatag agtggtttga tgccaacaag actttgcttc accaagtcat ccttgaagaa
      901 aattaaaccg gtttcctatt ctctcaggag atggagcatg ccttgaaagc cgttgtactg
      961 actcgatcac ttcccctctc agcccctgaa tccctggaag atgtgggtcg tcccagccca
     1021 gagcccaaag aacgagatct gcatgtgaag acagaagcca ctccgtcaat cccctagatt
     1081 ccaaataggt gcgggattgt tttcgccttg gaatcggtag atctcaacgc cttcacacga
     1141 aaaagcgcca cggtttcctg gaaattcagg ttcaccacct tctctgtatc aggtcgagga
     1201 tatgattaca caacacagat tactcacccg gattgctcgc tgggtagatg cgggcaaaat
     1261 tcaatccaca ctgaccgaac cactctctcc cctcaacacc gctcatttga gaacggccca
     1321 tactaaaatc gaataagtac gcatgatcgg aaagtttcct ctgaccggct ggcaccacta
     1381 gcggaaagat taaaaaatgt tgtggtggtt ctcaatacat gggtttccgg gcaacagctc
     1441 tattgagaaa gtaggaagat gcaagagcac tcgtgtaggc cggccccaac atgctagggt
     1501 ttcgctgatg ctcagccgcg gtgattcatg atgcccattc cttcccattc atcttcgttg
     1561 aagaacccgc cggcctggct gtgtgaactg ccgaaaaaag atccctggtc gagaccattt
     1621 gccgaaagcc tgttgaccca cttggatctc tttgaaggcg ccacgatact ggatgtggcc
     1681 tgtggagatg ggattcctgc cttttatctc gctcatcatg ttggaccagg tggccaagtc
     1741 gtcggaattg atgccaatca cgcgcaactc atccgagccc gcgccgttca ggggccgtat
     1801 ttcccctggc tggagttccg gcttggggat gtccgaacat tgccacccga tctcggccaa
     1861 ttcgatcgca tcacgggcaa cctgtccttt atgttttttc gacctcaccg tctggaagcc
     1921 cttcagcaat tggctcggtt tctcaagcct ggagggcaat tggttctcac atttccctcc
     1981 cttggaacct ttgattcttt atggaaacgc gtggaccggg aaatgaccca acaaggactt
     2041 acgaaagagc gagaggcatt tgcggactat ctcgccgaac ggccttcggc ggtggatgcc
     2101 cgaacatggc tggcagcctg cggattgatc aacatagacg tgatggtata tcccctggaa
     2161 gtgcacaccg gtccaggcca agagtttctg tatcacccgt tgctccgtgg gggattctta
     2221 gatgatgtct atgaatgctt cgacgatcaa acattggcag accaattcat ggatacgatt
     2281 tccctggaca ctcaaagttt cgtgccgctc ttcgcacaac gctgtgtcat ggctggttgg
     2341 ttgccggaaa agtaatggaa ttttaaatat cgatggatga tttccagcat gtcatcctca
     2401 gccgaaagcg aaggagctgt gcctgagttg agcattcgca gagctgaatg agatgcttcg
     2461 tttcactcac gatgacagag gagtgagcgg cgaagataca cgacacgaaa gacacaggtg
     2521 tccgccaaaa tattctcaaa aaaaatggag ctacatagat ggcagaagaa gtgacgccgg
     2581 atcgaatcat gcaattggga atggccttct ggggctccaa gacactgttg agcgccgtgg
     2641 aactcggcgt cttctctgag ttggccaaac agccgcttga cgctcaggcc ctgcaggcac
     2701 gtctggggct tcatccccgc agcgcgaggg atttttttga tgcgcttgtc gcactgggca
     2761 tgttggatcg gcagaatgga cactatgcca atacatccga gaccgatctg tttttagata
     2821 gagccaaacc cacctacttg ggagggtggc tggaaatggt caatttccgt ctttaccgat
     2881 tttggggaaa actgaccgag ggattgcgga ccgggctacc gcagaatgaa gtcaaagaag
     2941 gcaaaaattt ctttgaagaa ttgtacagcg atcctcggcg cctgaaagag tttttaagtt
     3001 ccatgacagg gttgagcatg ggagccagca gaaagatcgc ctctcaattt ccctggaaac
     3061 aataccacac cttcattgat gtcggatccg ctcaaggcgg attagcggta caggtgggat
     3121 tggcgcatga acatatcacc ggcggtgggt ttgatttacc cgtaacgggg ccggttttta
     3181 aggaatacgt ccaatccttc gggctgtcct cccgattgaa atttcatccg ggaaacttct
     3241 tctcggaccc ccttccaaaa gccgaggtgc tctccatggg ccatattctc cacgactgga
     3301 atttggaaga aaagaaaatg ttgatcgcca aagcttatga cgccttgccc ccaggcggcg
     3361 ctctgattat cttcgagtcg ttgatcgatg atgagcgccg ctcaaacgct ttcggcctcc
     3421 tgatgagttt gaatatgttg atcgagttgc caggggggtt tgactatacc ggcgcggatt
     3481 gcactgggtg gctgcgggaa gccggatttc gcgaaacccg cgtggagcat ttaatcggag
     3541 cggattccat ggtggtaggc atcaaatgag cctgggtttt tggcggattc tctcccgatt
     3601 gccgattgaa tgtagggata tccctcacgg cccacgagat agcaaaggtg atggagctaa
     3661 tcgttcccgt ttataagaat cctatgattg gtgattaatg caatccgatg gatgccgcga
     3721 cggatccttc aaacatcgct gagtctgatg tttctgcatc ccctcatacc ctctttctat
     3781 gggctcatcc cagccgtatc cggccatccc tgtggcacaa acaaacccca aaaagacaaa
     3841 ggttgttaga aattgatgac gatccggttg ccgtttcatc acatagacct atcgcaacaa
     3901 tcctgcagaa tgccaactac caccacacca ctgtcggcgg ttacacatgc aggaaggaat
     3961 cgaccctgat tccgaaaggt cggaggagaa aggtgaacac ttcgcggtgg gtcttcatgg
     4021 cgggaacgtt gatcattgta tttgcaggag gatggtacgg aatgatttgg atgccgggga
     4081 gcagttatca aggagccctg ccagtagcga ccgacgaaat acgtgacctg gagcaaaaac
     4141 tccataccca tgtccacacg ctgagcacgc aaataggaga acgcaacctc tttcactacg
     4201 acaaactcct gctcgcggcc gaatacattc gcgctgaatt caaagcggcc ggatatgatg
     4261 ccctcagcca aaaattcgac gtctccggaa acatctgtga aaacatcgaa gcggaggtac
     4321 gtgggacgaa tcgccgggag gacatcctgg tcattggcgc acactatgat tccgtcaaag
     4381 gaagtcccgg cgcgaatgat aacgggaccg ggatcgcggc catgttagcc ctggccaggc
     4441 agttcgccca atcccctgtt tctcgcaccc tcagatttgt ggccttcgct aacgaagaac
     4501 cccccttttt tcaaacccca agcatgggga gttgggtgta tgcccagcgc agtcgccaac
     4561 agaatgaacg cattgtcctc atgctcagcc ttgaaaccat tggctactat acggaggcac
     4621 cgagcagcca acgctacccg ccgccgctca gcctcattta cccgtcaacg ggaaatttca
     4681 tcgggtttgt gagtaacctt gcgaaccggg attgggtgca acgggcggta ggagcctttc
     4741 ggcaacacac ccagtttccc tcagaaggcg ccgcgttatt tgaatggttg cccggggtgg
     4801 gctggtccga tcattgggca ttctggcagg aaggattccc tgccctcatg gtcacggata
     4861 cggctctgtt tcgagacccg aactaccaca ccacaaagga tacacctgat catattcact
     4921 ttggccacct cgcccgcgtg gtcaccgggc tggaacatgt catcagggat ttggccaacc
     4981 aaccttgagg aatatcatga cgaaggaccc gtcttagacc cgattgattc cccccttggg
     5041 ctttccaata aaaaatataa aatattgtca acgaatgcgg ggtccctcgt catccccaga
     5101 tgatcggaga aaataaacag cacctggctc caggggatag gcgagatcaa tcggcttccc
     5161 ggcattttct cattcctctc atccattaac gcgcttcgcc gtaagacgac tccatctcct
     5221 ggggcatgct tggtaatctt cagacgcccg tcatcttgaa tttgtgctgc cgacatggtc
     5281 gacatggaat cccccgcaac cagcaaaaac cgtgtagttg gcggaggcgt cgcatgaacg
     5341 tccaacgcgg aggtcaattg cttggcccga tctaatgcct ttcgttgatg atccaacgcc
     5401 actctcctcc gctcctgcgg gtcggcgatg tcgggcaaca atatcttaag aatccggtcc
     5461 tgagatggat cggccaatcc ccatccctgc cgctcccaca aggttggatc aaacacattc
     5521 gtaaccggtt ggtcattgac atcaaataaa ggctgatgcc ggcttcgggg aagcaactga
     5581 tacaccgatg gcatcgtgcc cacgatggct gacggatatg tcgagagtag gggagcaggc
     5641 tttaacccat ccacgagaat tgtcagggtt tccaccgaac ccgcattggg tggaccaatc
     5701 accaccagat gatccacgtg tctggcgccc tcccaggtca cgggaggcag gctgccatcg
     5761 gcgggaagat cggccgcccc aaaacgtaga taataccggg ccaccagact tcccatggag
     5821 tgcgccacca gatcgaattt caccgggctt cgtttcattc cgaaccgtct gaaggtctcc
     5881 tcctggactt ccgcttcctt ggctttgata aaccggtcca aaccctgggc agattccacc
     5941 agatcgcgcc gccagtcgta cgcatattga aaacaggtaa aatgccggtt gccatattcc
     6001 accgcatgac tctccgccag ttgctggtcg cgatagcctc ccacaccgag ggtgcgtagg
     6061 atgttgtaat aggcattcag ttccagggga ataccaagaa acttcaaaac cacccggtct
     6121 aaaactccgg cctgcctcac cgtatcccgc aaatccttca aggctacgcc aggcgccata
     6181 ggcaatgcca ataagcgcgc tccttcggcc gttgtcggat cgaccgctcc cgaaccgaat
     6241 gccccccaga ccacttctcg ggtatttccc tcttctaagc gggaacccag gattccggga
     6301 atgacgatga ccggattacg aagggggtcc tcctgctgcg ccaatttgtt atacagattg
     6361 cccagatccg gaacggtttg gaaggccgaa cagccgatta gcgacaacag gccaaccacg
     6421 caaaggagaa aaattcggat catcaaaaaa atcctccagc cgcggttcgc gggacgagaa
     6481 gccccggacc tatggctttc aataaagatc gtgaccctcc tgtagtcttt taatccctgg
     6541 cttg
//
LOCUS       DATIAL010000208         3257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1112185, whole genome shotgun sequence.
ACCESSION   DATIAL010000208 DATIAL010000000
VERSION     DATIAL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112185"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1060)
                     /gene="holB"
                     /locus_tag="VLA60_06555"
     CDS             complement(29..1060)
                     /gene="holB"
                     /locus_tag="VLA60_06555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HSF09056.1"
                     /translation="MAFREIVGHQRPIKWLQTAVTTHHLGHAYLFHGEPTIGKRLTAM
                     ALAQYLHCEAPQETPMPDACGFCRSCHQISQATHPDLLMIQPKDEQKANPKITIDQIR
                     DIEHLVIYRPLVGTHKVCFIDQADSMTPESANALLKTLEDPPDHCLFLLISSRPEHLL
                     PTIRSRCITLRFSPLSFASIYEYLKNQTDLEASDARLIAVFSEGRLGSALSCDPTELK
                     VKLRQYWALLLGEPTVSASRVMDMSEGLVKSNQIQEAIHWFWSGLRDLLLLTLDDSLP
                     PTLFTDQEMAIRALARQMTPWSVLPLIDELNQLERGQQRNLNVQIGLEQFFFHLHDQL
                     ELSRSRELV"
     gene            complement(1091..1813)
                     /gene="tmk"
                     /locus_tag="VLA60_06560"
     CDS             complement(1091..1813)
                     /gene="tmk"
                     /locus_tag="VLA60_06560"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSF09057.1"
                     /translation="MKPRNQEKTNQDLQLGLFITFEGTEGSGKTTQCRRLAKTLRFQG
                     YQVLETREPGGTPLAEAIRRLLLSHARTQSPGEAITPACETALILAARAQHVAHVIRP
                     ALAKGMIVLCDRFFDSTLAYQGYGRNLDADFLHQSHQLVTDGLTPDLTLLLDLPIQDG
                     LTRRRKSKHQNRLDRESLNFHQRVRQGFIMLAKQNPQRIQKFDARQSPDCLTLQIASV
                     TANLIHAKKSLVAHSTLKSMPK"
     gene            1982..2656
                     /locus_tag="VLA60_06565"
     CDS             1982..2656
                     /locus_tag="VLA60_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSF09058.1"
                     /translation="MSNFRASLKRYFLTGLLVITPIWGTILILKTLFVTVDSILGTAL
                     ADLVLPDYYFPGLGILALLLLIFSTGVLATNFFGKRLVTRWEEFLDRVPVVRGIYATL
                     KSMMDILSFKQREKYDKVVMIQFPKNGHYCLSFVTGETRGEIQKLAPEPLVHVFVPTS
                     PNPTSGYFLLVPEREIIPVDMGVEEAMKLIVSGGFYSPSDKPSSIEIAKKRDRIILKD
                     SPKVQT"
     gene            2692..>3257
                     /locus_tag="VLA60_06570"
     CDS             2692..>3257
                     /locus_tag="VLA60_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HSF09059.1"
                     /translation="MNCKKYTAAIILAAGRGTRMNSSLPKVLHCIAGRPMVWYAATLA
                     RQVATQTVAIVVGYGSEQVQAYLEHEKVNFEPFEIVKQDKQLGTGHAVQQAYPVFTRN
                     ASQKIDQFLILNGDTPLLTKDTLTALIDYHQSEDATLTLLTTLLPKPGGYGRVVRTHS
                     GEVCRIVEDQDASPKEKNISEINVGTYVV"
BASE COUNT          829 a          699 c          847 g          882 t
ORIGIN      
        1 gaaccgagga gtaacggaca actgttggtt agacgagttc tcgtgaacgt gataattcca
       61 attggtcgtg aagatgaaag aagaattgct ctaatccgat ttgcacgttc aggtttcgtt
      121 gttgtcctcg ctcgagttgg ttgagttcat cgattaaggg cagcaccgac cacggagtca
      181 tctgtcgggc caaagcgcgt atggccattt cctggtccgt gaagagcgtt ggaggcagcg
      241 aatcgtccag ggtaagtaac agcaaatccc ggagtcctga ccaaaaccaa tgaatcgctt
      301 cctgaatttg gttagacttg accagacctt cactcatatc catcacacgg gaagctgaaa
      361 cggtgggttc ccccaataac agagcccaat attgacggag tttgaccttc aattcggtag
      421 gatcacaaga caatgctgaa cccaaacggc cttccgaaaa taccgcgatc aatcgagcat
      481 cgctggcttc caagtccgtt tggtttttga ggtattcata gatgctggcg aaggaaagag
      541 gggaaaagcg cagcgtaatg caacgggaac gaatggtggg caacaggtgc tccgggcgac
      601 tggatatcag gagaaagagg caatggtccg ggggatcctc aagggtcttc agcaatgcgt
      661 tagcggattc cggtgtcatc gaatcagcct gatcaatgaa gcacacttta tgagtaccca
      721 cgagaggacg atatatgacc agatgttcga tatctcgaat ttgatcgata gtaatttttg
      781 gattggcttt ttgctcatcc ttcggttgaa tcattaagag gtcgggatga gtggcctgtg
      841 aaatttgatg gcatgatcgg cagaagccgc aggcatcagg cataggtgtc tcctgaggtg
      901 cctcacaatg tagatattgt gcgagtgcca ttgcggtgag acgtttgccg atggtcggtt
      961 ctccatgaaa caggtaggca tgtcccagat gatgggtggt gactgctgtt tgcagccatt
     1021 taatgggtcg ttgatgtcct actatttctc gaaacgccat ggatatatgc ccttttcgtg
     1081 tgccttggtg ttatttgggc atggacttga gggtggaatg agccacaagt gattttttag
     1141 catgaatgag gtttgccgtc actgaggcga tttggagtgt caggcaatcg ggtgactgtc
     1201 tggcatcaaa tttttgaatt cgttgagggt tctgtttggc aagcataatg aagccttggc
     1261 gcactcgttg gtgaaaattc aaagattccc gatcaagtcg attttggtgt ttggacttcc
     1321 gtcgtctggt caatccatct tggataggaa gatcgagtaa aagcgtaaga tctggagtca
     1381 gaccgtcagt cacgagttga tgggactggt gaagaaagtc ggcgtcaagg ttccgtccat
     1441 atccctggta ggctaaggtt gaatcaaaaa aacggtcaca cagtacaatc atacccttcg
     1501 caagagctgg tcgaataaca tgggccacat gctgggctcg ggcagccagg ataagggcgg
     1561 tttcacatgc gggagtaatg gcctcacccg gtgattgagt cctggcgtgg gataggagca
     1621 ggcgacgaat ggcctcggcc agaggcgtgc cacccggttc cctcgtttcc aacacttgat
     1681 acccctggaa acgaagagtc ttggccaatc ggcggcattg ggtcgttttc ccggaacctt
     1741 ccgttccttc aaaagtaatg aataatccga gctgcaggtc ctggttagtc ttttcctggt
     1801 ttctcggttt cattggcgca ttctaggtgt gatcctaatc taagtcattg aaattcgcaa
     1861 gaaaagtctt gcaggagtcc tgtcctgatt gtaagatgca gtagcacttt atcatcacct
     1921 ccatttctct cgcatggggt tgtgcccgct tcctggagcg gcttaagacg acttttttcc
     1981 catgagtaat ttccgggcct ctcttaaacg ctattttttg acgggcctgt tggtgattac
     2041 cccgatctgg ggaacgattc ttattttaaa aaccctgttt gtgacggttg atagtatcct
     2101 tggaaccgca ttagcagact tggtattacc ggattattat tttcctgggc tgggtatttt
     2161 ggcattattg cttcttattt tttctactgg tgttctcgca acgaattttt tcggaaaacg
     2221 gttggtcacc cgctgggagg aatttttgga ccgggtccct gtcgtcagag gtatttatgc
     2281 cacattaaag tcgatgatgg atattttgtc attcaaacaa cgggaaaaat atgacaaagt
     2341 ggtgatgatt caatttccta agaacggcca ttattgttta tcatttgtca cgggggaaac
     2401 tcgcggagaa attcagaaat tagcaccaga gcccctggtt catgtctttg tccccacgtc
     2461 ccccaatccc acctccgggt atttcctctt ggttcctgag agagaaataa ttcctgtgga
     2521 tatgggagtg gaggaagcca tgaagttaat cgtatccggg gggttttatt ctccctctga
     2581 taaaccctct agcattgaaa ttgctaaaaa acgggataga ataattctca aggattcacc
     2641 aaaagtccag acttaataaa ttcaatacct tacgaatcga ctcgttcaaa tatgaattgt
     2701 aaaaaataca ctgcagccat cattcttgct gcggggcggg gaacccggat gaattcatct
     2761 ttgccaaaag ttttgcactg tattgccgga cgacccatgg tatggtatgc cgcaacatta
     2821 gccaggcaag tggcgactca gactgttgcg atagtagttg gctacggatc tgaacaggtt
     2881 caggcctatt tggagcatga aaaggtcaat tttgaaccgt ttgagatagt gaaacaggac
     2941 aaacaattgg ggacaggaca cgccgttcag caagcctatc cggtgttcac caggaatgcc
     3001 tcacagaaga ttgatcaatt cttaattctc aacggggata ctcccttatt aacaaaggac
     3061 acactcactg ccttaattga ttatcatcaa tccgaggatg ccacactgac gttattaaca
     3121 actctgttac cgaagccagg tggatatggg agggtcgttc gaactcactc cggagaagtc
     3181 tgtcgaatcg tggaggacca ggatgcatca ccaaaagaaa aaaacatttc ggagattaat
     3241 gtcggaacgt atgtggt
//
LOCUS       DATIAL010000209         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1004552, whole genome shotgun sequence.
ACCESSION   DATIAL010000209 DATIAL010000000
VERSION     DATIAL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004552"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="VLA60_06575"
     CDS             <1..1807
                     /locus_tag="VLA60_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09060.1"
                     /translation="GTSHVILKSNFQGHLSPTYQLTSLRSEDQLQVTLGNLTYQDPSL
                     TATLPSIRLSLKTKEDVFKQDYLLESLRLSSEEILDLGMNARYQQESQRFDVDLQLPL
                     LHVGNLLPHLSGPLMKGVDEINPKGRISLTLRAAGKVPQEADLKKLTLPLGINGKLTM
                     RDLEGAVSGYRVQGGNGTMTMGYSPRATPQTQLTTDLTLNRIHLPDTLPIRELANTSL
                     HVNMSSPDLNEVQIDPIHVVSKGVDLSIKSTVVGLRELLSSTSPRGTQLAKLFAQLHT
                     RLALDVETFQEALQPLGIQGNGKALVTLSMLKKEQGSLDASVEIGSETLSLTRDGTEL
                     HDMNGGIQLRKSLAWKPDGLPSPLRKAFQPSDRIAQLKSFSRKGQTLTIDRLQLGPFT
                     VENLSTNLAFEQQSLKMQNLAMNVLGGGIGGNVIIAAEHPLRLSAEFEVANLDANRLI
                     QTESKIKGDSNIAATMGLTTILQETTGAVDLSRMECNLHITHIGNEALDRFLVFLDPE
                     GSKPTISNVRAQLKFANPSRVTVEVARGQLNLTIHFQGSLIPTFRLERIPIAKMKNIE
                     KLTAAIPNWEDIAKILELIGAESYSFTPEGEIVLQ"
     gene            2448..>3273
                     /locus_tag="VLA60_06580"
     CDS             2448..>3273
                     /locus_tag="VLA60_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HSF09061.1"
                     /translation="MEGSPKIELKKIPSGYRGTLKTLEHIIDLIKQGAKDFHVRQTAI
                     DILLQRGVRAKDYLGEIKALFEWVQQHVRYTKDTFRVEVLHSARRMLELRAGDCDDMS
                     ILLGAMLESIGHPVRLALSGPDPLKQDLFTHIYLEVFHKGRWVPLDATMPYPMGWAPR
                     TLVKKIIPMERRPDMMAEDLEFQGIGAAGVVPDWLRGLIRAVRSEAIQPKDPRVKSLW
                     DLLRQRQLLRRSPRLKAVLRRIWSRGLSARPRPRTAGRIVRSLRRWGLLPPKSLRGA"
BASE COUNT          834 a          874 c          846 g          719 t
ORIGIN      
        1 cgggaccagc catgtgatcc tgaaatctaa ctttcaaggc catctctcgc cgacctatca
       61 actcaccagc cttcgctcgg aagaccagct ccaggtcaca ctcggcaacc tgacctacca
      121 ggatccctct ctgacggcca cccttccttc aatcagactg tcattaaaaa ccaaggaaga
      181 tgtgttcaaa caggattatt tactggagag cctgcgactg agcagcgaag aaatattaga
      241 tttggggatg aatgcccgat atcagcagga gagccaacga tttgatgtag atcttcagct
      301 accgcttctc catgtcggca accttctccc gcatctgtcg ggaccgctca tgaagggggt
      361 agacgaaatc aatccaaaag gacggatcag tctgacactg cgagcggcgg gcaaggttcc
      421 ccaggaagcg gatctcaaga aattgaccct tccgttggga atcaatggaa aacttacgat
      481 gcgggatctt gagggagccg tctcgggata tcgggttcag ggaggcaacg gcaccatgac
      541 catgggctat tcgccacgag ccacgcctca aacccaactc accacggatc tcacactcaa
      601 tcgtattcac ctgccggaca ccctcccgat cagggaatta gccaacacat ccctgcacgt
      661 gaatatgtcg tcccctgatc tcaatgaagt ccagattgac ccgatccatg tggtatcgaa
      721 gggagtcgac ctttccataa agagcacggt ggtgggcctg cgtgagctgt tgtcctccac
      781 ctcgcctcgt ggcacccaat tggcaaaact ctttgcgcaa ctgcacaccc gcctggctct
      841 ggatgtggag accttccaag aagcgctcca accgttgggg atacagggaa acggcaaggc
      901 cctggtcacc ctgtccatgc tgaaaaaaga acaaggcagt ctggacgcct ctgtggagat
      961 cgggtctgaa acactctccc tgactcggga tggcacggaa ctgcatgaca tgaacggtgg
     1021 aatacagctt agaaaatcac tggcatggaa accggatgga ctcccgtctc cgctaagaaa
     1081 ggcatttcaa ccgtccgacc gaattgccca acttaaatcg ttttcccgga aaggacagac
     1141 cctcaccatt gatcgtcttc agctcgggcc attcacggtc gaaaacctct cgacgaattt
     1201 ggcgtttgag caacagtctc tgaaaatgca aaatctcgcc atgaatgtgt tgggtggagg
     1261 gattggggga aatgtcatca ttgccgctga acatcccctg cgtttatcgg cggagtttga
     1321 agtggcgaat ctggatgcca accgactcat tcagacggaa tctaaaatca aaggggatag
     1381 caatattgcc gcaaccatgg gactcacaac catattgcaa gaaaccacgg gagccgtgga
     1441 tttgagccga atggaatgca acctgcacat tacgcacatc gggaacgaag cgctggatcg
     1501 cttcctcgtg ttcctggacc cggaaggaag caaaccgaca atctccaatg ttcgtgcgca
     1561 attgaaattc gcgaatccct cacgggtgac ggtggaagtg gccagaggcc aattgaatct
     1621 gacgatccac ttccaaggca gtctgatccc cacctttaga ttggagcgca tcccgatcgc
     1681 caagatgaaa aacatcgaaa aactcaccgc cgccattccc aactgggaag acatagcaaa
     1741 gatattggaa ttgatcggag ctgaatccta ttccttcacc cccgaagggg agatcgtgct
     1801 tcagtaagtg acaatcctct tatctagtgt catgctgagc tgaaggcgaa gcatctgggt
     1861 aaggcatgtg ccgtttaagc tgggctcata tccttcgttt cactcaggat gacaacgtga
     1921 aggaccattg tcagcaagga agcgctcctc aaattgctct atcgaactat tcaaagaatg
     1981 acaagaaata aacgtcccca ttccagagta gaaggatgca gacaattaat ttattttgtt
     2041 gtgcctaaat tagtatctcc cagattgttc tgcgcgcttg cctcgcattc cgcctgactc
     2101 tgcctcatag ggttaatgac agtagggttt cgccgtttct agccaactcg gcacccacac
     2161 aggcataaat tttatgatct cactcataat ttttatgacc cggtctaagc atcctccttc
     2221 tccctataga ggagagagtc tgttcccata ctctccccgc cataattctt atgatccccc
     2281 tccattctat cgtttcacaa gtctgtgcac agcgattcaa tgttccttta aaagcaacgt
     2341 gttaaagagt ctccacgcct ctgctcagtc gtctagcaca aggcttgcgt catgttgttc
     2401 tacaagcagt agggaacgtt gacatggcaa tgaacgaatg aacatgcatg gaggggtcac
     2461 ccaagatcga attgaaaaag atcccgagcg ggtatcgggg aacgttaaaa acccttgagc
     2521 atatcatcga tctgattaaa cagggggcaa aggattttca tgtacgccag accgcaatcg
     2581 atatcctgct gcaacgaggc gtcagagcca aagattacct gggtgaaatc aaggcgctgt
     2641 tcgagtgggt gcagcaacac gtgcgctata ccaaagacac gtttcgcgtg gaggtgctcc
     2701 actctgcccg gcgtatgttg gaactccgtg cgggcgactg cgatgacatg tcgatcctgc
     2761 tgggagcgat gttggagtca atcggccatc cggtgcggct ggcgctcagc ggacccgacc
     2821 cgcttaagca agaccttttc acccacattt atctggaggt ttttcacaaa ggtcgctggg
     2881 ttccgttgga tgccaccatg ccctatccaa tgggctgggc gccaaggaca ctggtgaaaa
     2941 agattatccc aatggagagg agacccgaca tgatggcaga agatttggaa tttcagggca
     3001 tcggtgctgc cggggtggtg ccggactggc tgagaggttt gatccgggca gtgcgaagcg
     3061 aggccataca gccgaaagac ccgagggtaa agtctctgtg ggacttactg cggcaaagac
     3121 aactgctgcg ccgaagccca aggcttaagg cggtgctgcg gcgtatctgg agccgagggc
     3181 tgtccgctcg acctcgcccg cgcacagcgg gtcggatcgt gcggagttta cggcgttggg
     3241 ggctcttgcc accgaagtca ctcagagggg cgg
//
LOCUS       DATIAL010000210         5141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1112466, whole genome shotgun sequence.
ACCESSION   DATIAL010000210 DATIAL010000000
VERSION     DATIAL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112466"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VLA60_06585"
     CDS             complement(<1..261)
                     /locus_tag="VLA60_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09062.1"
                     /translation="MIGGQFWKVVGLCVVCLMVFGGFGSATWGAASPEPSAARDLPEL
                     RLSLREAMDAAVDQNPTVRLFNERITQAQDVANTQLGELLPNL"
     gene            complement(284..3535)
                     /locus_tag="VLA60_06590"
     CDS             complement(284..3535)
                     /locus_tag="VLA60_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF09063.1"
                     /translation="MNLVHSAIRYPVSTAVGVLLVVLFGIVALVKLPVQLAPDVEKHE
                     VTVDTVWPGGSPHEVEREIIDEQEEQLKGVEGLEKMFSESSYGQGKVILRFPTGTNTD
                     TALLQVSNRLNQVKEYPLEADEPVISSADTRGAAMAWFILDTLEGNPVNIETMRDFAE
                     DIIKARFERVQGVAASNVYGGRERELRVTVDPAKLASRALTIMDLARALDQENRDYSA
                     GDFDEGKRSYVVRTVGEYLNPQDVENVIIARRDGASVYVRDVATAHIDYKDPTVVVRQ
                     KGHPAIAINAIRQTGANVLEAMDGLREAASELNEEILNARGFQLFQVYDETTYINRSL
                     TLVQQNLIVGSLLAIFILYLFLRTGSSTLVVGLAIPISMMGTFIMLWALGRNFNVISL
                     AGMTFAAGMLVDNSIVVLENIYRHRELGKSLFQSAYDGTVEVWGAVLASTLTTIAVFV
                     PIVFMEEQSGQLFRDIAIAISAGVGLSLVVSMTVIPSLSARVLTTVKKSKGERAPATW
                     STAGFAESGSTAGRHSWLDRTKEALGSLVYGLSGSVFWRSVTILGFTSLAILGSWALL
                     PKAEYLPQGNRNLVIGILLPPPGYNTHEFVKMGQPIESTLSPYWDVKPGSPEEAALDG
                     PSIANFFYVARGRSVFMGGRVNDPSRVKELIPLFRRATADLPGVIGIITQRGLFERGL
                     GEGRNIDIEITGPELDTLVQLGVQVFGQVKGVLPEAQVRPIPSLDLGSPEVRVTVKRD
                     RAADVQMTNQELGFTIDALVDGAKASDYQFEGDEVDLTIRGVDRYANQTQSLNSIPIY
                     TKGGQLTTVGNIADVTLVSGPEQINHIERERSIVIQVIPPVEMALEEAMDVIRTQILG
                     PLKESGTLGRLYNVRLSGTADDLTETYDALKWNFLLAFTITYLLMAALFESFLYPLVI
                     IFTVPFAAAGGFLGLSLVNNFIAYQPLDVLTMLGFVILIGVVVNNAILVVHQALNFMR
                     GTEEGDSFGEESGQGLPLREAIRESVKIRVRPIMMTTLTTLFGLFPLVVSSGAGSELY
                     RGIGSVVLGGLLVSTVFTLIVIPALFTLVVDTQRKWAEARNPQLAEQPSGGAL"
     gene            complement(3532..4638)
                     /locus_tag="VLA60_06595"
     CDS             complement(3532..4638)
                     /locus_tag="VLA60_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF09064.1"
                     /translation="MTHWTKRRLWCVLVLIIGLPGWQGCSEQEAAQPPGGRPPAPVKV
                     VTVSNKQVQRSVSLVGTAEPRKRSLVASEVAGLVKSFPVKEGQFVNKGQLLAGLRTDT
                     LEIRLDSAVALQHEARARYEQAQRDLERVKLLSAKELVTQKELDDAMAEESALGKRLS
                     QLKAEIRLVQNQLTKSTIMAPFGGWITKEYTEIGQWVEEGGPVVELVDLAHVEVQVPL
                     PEEYVRDVRVGDPVLAGFDGLPGVEIKGKVLSIIAQADRAARTFPVKVELANPDLHIK
                     SGMVARVKLALGAPYQAVVIPKDALVLKGGKEFAFIVANNTVTQIAVIPVAHFEDVVE
                     VQGAIEEGMQIVVEGNERLLPGQSVRILEEPVKA"
     gene            complement(4643..>5141)
                     /locus_tag="VLA60_06600"
     CDS             complement(4643..>5141)
                     /locus_tag="VLA60_06600"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09065.1"
                     /translation="MGTVYRFFKSKEDIYISLVEAKVEELLRLLEEATRNHVSAQERL
                     RAVIHVKLAFADQNRAFFRIYVSEWSGFEWTVKSAFGERVWKRYLAQIDLVANLITDG
                     IKSGEFRKVNPKETSLAFHGMLNSTIYLWILQAGPKDSLVDKGKWLGTLFLGGLGTAT
                     KKRSM"
BASE COUNT         1276 a         1552 c         1138 g         1175 t
ORIGIN      
        1 aagattgggc aataattcac cgagctgtgt attcgcgaca tcctgcgcct gcgtaatacg
       61 ttcgttaaac aaacggaccg ttggattttg atcgacagcc gcatccatcg cctctcgcaa
      121 actcagccgc aactcaggaa ggtcacgagc cgctgaaggc tccggggaag ccgcccccca
      181 ggtcgcggac ccgaatccgc caaacaccat tagacacacc acgcatagcc caaccacctt
      241 ccaaaactga cctcctatca tatcttgccc cttcgcctcc agattacaaa gcgccgcccg
      301 acggctgttc agctaattga ggatttctgg cctctgccca cttgcgttgc gtatcaacga
      361 ccaaggtaaa caatgccgga ataacaataa gggtgaacac tgtggagacc aggaggccac
      421 ccagcaccac acttccgatc ccccgataca gctcggagcc cgcgccggat gagaccacaa
      481 gcggaaagag gccaaagagc gtcgtgagtg tcgtcatcat aatcggccgg acacggattt
      541 tgacggattc acgaatggcc tcccgaagag gtaacccttg tcccgattct tctccaaacg
      601 aatccccctc ttccgttccc ctcatgaaat ttaaggcctg gtgcaccacc agaatggcat
      661 tattgaccac cacgccgatg agaatgacaa agcctaacat ggtcagcaca tctaatggtt
      721 gatacgcgat aaaattattg accagagaaa gcccaaggaa tccaccggct gcggcaaacg
      781 gcacagtaaa aataatgacc agaggataga gaaaactttc gaacaacgcg gccatcagga
      841 ggtaggtaat ggtaaaggcc aacagaaaat tccattttaa cgcatcatag gtttcggtaa
      901 gatcatcggc cgtccccgac aagcggacat tatacaaacg tcctaacgtc ccgctttctt
      961 tcaatggccc aaggatttgc gttcgaatca catccatcgc ctcctccaac gccatctcaa
     1021 ccggcgggat cacctgaatc acaatggatc gttcccgttc gatatgatta atttgttccg
     1081 gacccgagac taatgtgaca tcggcaatat tgccgacggt cgtcagttga ccgcctttgg
     1141 tataaatcgg aatggaattg agcgactgcg tctgattagc ataccggtcg actccacgaa
     1201 tcgtgagatc gacttcatct ccctcaaatt ggtaatcact ggcctttgcc ccatcgacca
     1261 gggcatcaat ggtaaatccg agctcctgat tcgtcatttg cacatccgcc gcacgatccc
     1321 gtttgacggt aacccggact tccggacttc ccaaatccaa actggggatg ggtctgactt
     1381 gtgcctcggg gagcacccct ttcacctgac cgaacacctg aacacctaat tgaacaagcg
     1441 tgtccaattc cggcccggtg atctcaatat cgatatttcg cccctcgccc aagccacgct
     1501 cgaacaggcc gcgttgtgtg atgatcccaa tcacgcccgg aaggtccgcc gtggctcgac
     1561 gaaacagggg gatcaattcc ttcacgcggc tcggatcatt cacgcgacca cccataaaga
     1621 cggatcgccc ccgtgccaca tagaaaaaat tggcaatact ggggccatcc aaagcggcct
     1681 cctccgggga accgggcttt acatcccaat aggggctgag ggtcgattca atcggctgtc
     1741 ccattttgac aaattcgtga gtgttatacc ccgggggagg cagcagaatg ccaatgacaa
     1801 gattccggtt tccctgagga agatactctg ccttcggtaa caaagcccag cttcccagta
     1861 tcgcaagaga ggtgaatccc agaattgtca cacttcgcca gaagacgctg ccactcaaac
     1921 catacaccaa agaacccaaa gcctctttgg tccgatccaa ccaggaatgc cgtcccgccg
     1981 tcgaaccact ctccgcgaat cccgcagtag accacgtagc gggtgctcgc tcccctttgg
     2041 actttttcac cgtggttaac actcgtgcag acaatgatgg aatcaccgtc atcgagacca
     2101 caaggctcaa acccactccc gcactgatcg caatagcgat atcccgaaac aattgaccgg
     2161 attgttcttc cataaacacg atgggaacaa atacggcaat agttgtgagg gtactggcca
     2221 acaccgcacc ccacacctcc acagtgccat cgtatgccga ttgaaataac gatttgccca
     2281 attcccgatg acgataaata ttttccaaca ccacaatgga gttatccacc agcatcccgg
     2341 cagcaaacgt cattcccgct aaactgatga cattgaaatt ccggcccaat gcccacagca
     2401 taataaacgt ccccatcatg ctgattggaa tggccaaacc gaccaccagg gtgctactgc
     2461 ccgtgcggag gaataaatac aaaatgaaga tcgccaataa actcccgacg atcaaatttt
     2521 gctggacaag agtcagggat cggttaatgt aggtggtttc atcatagacc tgaaataact
     2581 gaaacccccg cgcattcaaa atttcttcat tgagttcact agcggcctcc cgcagaccat
     2641 ccatggcctc caatacattg gccccggtct gccgtatcgc gttgatagcg atggctggat
     2701 ggcctttttg ccgaaccacc acggtcggat ccttgtaatc aatatgggcg gtcgccacat
     2761 cccgcacata caccgacgcg ccatcacgcc tggcaatgat gacattttcc acatcctggg
     2821 ggttgagata ttcccccacc gtacgtacga catatgaccg tttcccctca tcgaaatctc
     2881 cggcactgta atccctattc tcctgatcta acgctcgagc caggtccata atggttaacg
     2941 ctctggacgc taatttagcc ggatcgaccg tcacccgaag ctcccgctcc cgcccaccgt
     3001 agacattcga agccgcgact ccctggaccc gttcgaaacg ggctttgatg atatcctctg
     3061 caaaatcccg catggtttca atgttgacgg gattcccttc tagagtgtct aaaataaacc
     3121 aggccatggc ggccccacgg gtatccgcgc tgctaatgac gggttcatcc gcctcaagcg
     3181 ggtattcctt gacctgattc aaccggttgg aaacttggag taaggcggta tccgtattcg
     3241 ttcctgtggg aaatcgaaga atgacttttc cctgaccgta ggaactttcg ctgaacattt
     3301 tttccagtcc ttcgacacct ttaagttgct cttcctgctc atcgatgatt tcacgctcga
     3361 cttcatgcgg gctccccccg ggccaaacgg tatccacggt gacctcatgc ttttccacat
     3421 caggcgccaa ttgaaccggg agcttcacca acgccacaat gccgaacaac accactaaga
     3481 gcacacccac tgccgtgctg accggataac ggatggcgga atgaaccaga ttcatgcttt
     3541 taccggctcc tctaaaattc tgaccgactg cccgggcagg aggcgctcat tcccttccac
     3601 gacgatctgc atgccttctt caatagctcc ctgtacttct accacgtcct caaaatgggc
     3661 tacggggatc accgctatct gcgtgaccgt attattggcc acaataaagg cgaactcctt
     3721 tccccctttg agcaccagag catccttggg gatcacgacc gcctggtaag gagcccctag
     3781 ggctaatttg accctggcca ccatcccgct tttaatgtgt aaatcagggt tagccaattc
     3841 cactttaacc ggaaatgtcc gtgccgcccg atcggcttgt gcgatgattg acaaaacctt
     3901 tcctttgatc tcaaccccgg gcaagccgtc gaaacccgct agcaccggat ccccgaccct
     3961 gacatcccgc acgtattctt ccgggagcgg gacctgtacc tcgacatgtg ccaggtctac
     4021 caattccacc actggtccgc cttcttcgac ccattggccg atttccgtat attcttttgt
     4081 gatccaaccg ccaaaaggcg ccatgatggt ggatttcgtc aattgatttt gtacgaggcg
     4141 aatctccgct ttcaattgag atagacgctt tcccaatgct gattcctctg ccatcgcatc
     4201 atccagctct ttttgcgtga ccagttcttt ggcagacaac agtttgacac gctccaaatc
     4261 cctctgggct tgttcatatc gggctctggc ttcatgttgt aacgccacag ccgaatcgag
     4321 tcgaatttcc aacgtgtctg tccgaagacc ggcaagcagt tgtcctttat tcacaaattg
     4381 gccttctttg accggaaagg atttgaccag gcctgccact tcactggcca cgagacttcg
     4441 cttgcgtggt tccgctgttc ctactaatga gacggatcgt tgaacctgct tgttcgacac
     4501 cgtcacaacc ttcactggtg cgggcggacg tcctcccggc ggctgggcag cctcctgttc
     4561 gctgcatccc tgccatcccg gaaggccaat gatgaggaca agaacacacc agagccgcct
     4621 ttttgtccaa tgtgtcatag gttcacatgg accttttctt cgttgccgtt ccaagaccgc
     4681 caagaaacaa ggtgcccaac catttccctt tgtccacaag ggagtctttc gggcctgcct
     4741 gtaaaatcca aagataaatt gtagaattta acatgccatg aaaggccaac gaggtctctt
     4801 tgggattcac cttgcgaaac tccccgcttt tgatgccgtc agtaatgaga ttcgcgacaa
     4861 ggtcaatctg cgccaaatac cgtttccaca cccgttcgcc aaacgcactt tttaccgtcc
     4921 attcaaaacc gctccattcg gagacataaa tacgaaagaa tgcgcggttt tgatccgcaa
     4981 aggctaactt cacgtgaatc accgcgcgca atctttcctg agctgagaca tggtttcgtg
     5041 tcgcttcctc caacagacgg agtaattcct cgactttcgc ttcgacaaga gagatataaa
     5101 tatcttcttt gcttttaaaa aaccggtaca cggtccccat a
//
LOCUS       DATIAL010000211         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1543227, whole genome shotgun sequence.
ACCESSION   DATIAL010000211 DATIAL010000000
VERSION     DATIAL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543227"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VLA60_06605"
     CDS             <1..679
                     /locus_tag="VLA60_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HSF09066.1"
                     /translation="KFLIQKYAIATTPGLTLTDAHPLDREQVNLLSIGLSEGVQGFSP
                     LPNTQQEVKKLQDLFGGKTLLNEEFRIPNLEQDMKEENFTIIHIASHGKFAKEPKNSF
                     ILTYDKKLSMDHLRELIGLFQFRQIPLDLLTLSACETAAGDEQSALGLAGVAVKAGAR
                     SALATLWFINDQASSILINEFYVRLKEPSLSKAQALREAQEALLAHPIYRHPSYWAPF
                     LLINNWL"
     gene            complement(1086..1220)
                     /locus_tag="VLA60_06610"
     CDS             complement(1086..1220)
                     /locus_tag="VLA60_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09067.1"
                     /translation="MTDNKWEPVEMTGKGIKALKRTGAEDRSFIGTGKKPVLANAGWS
                     "
BASE COUNT          666 a          718 c          594 g          647 t
ORIGIN      
        1 aaaattcctc attcaaaaat atgccatcgc gacgactccg ggactgacgc tcaccgatgc
       61 ccatcccctg gaccgggagc aggtcaatct gttgtcgatt ggactgtccg aaggcgtgca
      121 gggattttca ccgcttccca atacgcaaca ggaggtcaaa aagcttcaag atctctttgg
      181 cggaaagact ctcttaaacg aagaattccg cattcccaac cttgaacagg acatgaaaga
      241 ggaaaacttt acgattattc atatcgcctc acatggaaaa tttgccaagg agcccaagaa
      301 ttctttcatc ctcacgtatg acaagaaact gagcatggat catctcagag aactcatcgg
      361 gttatttcaa ttccgacaaa tccccctgga tctcttaaca ttgagcgcct gtgaaacggc
      421 agccggagac gagcagtccg cactgggcct ggctggagtg gccgtgaaag cgggggcgcg
      481 aagcgccttg gccactttat ggtttatcaa tgaccaggca tcctcaatcc tgattaatga
      541 attttacgtc cggctaaaag aaccttcgct ctccaaagcc caagcccttc gtgaagccca
      601 agaggcccta ctcgctcatc ccatctatcg gcatcccagt tattgggcac ccttcctcct
      661 gatcaacaac tggctctaat ggggctgttg aaaaaagccg ccagcggcgt tccctgcctt
      721 cgccgggccc ccttcgccga agtcgctacg aaggccgaaa gcggcttcgc gtctacgcgc
      781 tgaagcacgc ttcgaggcgc aagcacgcag gcaggtcacc atttttccgt gctcacgtac
      841 tggaagtacg ctctgcgggc aaaaatgact gcggccttgc tggacgaacc cttcgaaagg
      901 ctcaggacat gcttttttga accggcccga ggcctcaatt atgcaacgtg ctgtgggtca
      961 atatgccctt ggaaatgatt attattcaac actcccctaa tcgtttattt ctcagactga
     1021 tccccggatt ggcccaacca tttttggtct tgtttcgagg gccgcctttc tcaaaaatca
     1081 ggcagtcacg accagcccgc atttgccaga accggtttct taccggttcc aataaacgat
     1141 ctatcttccg ctcctgtccg ttttaaggcc ttgatcccct tcccggtcat ctcgaccggt
     1201 tcccatttat tgtctgtcat tcccgccggc tgtaagcagg gagccacccg aaacagacca
     1261 aaagagatgc cgcataacca acgtggaaag gacaagagta ttggccacac tggtcaggct
     1321 taatagctga cttgtgcagc ctcaagaaag acagccattg acacagtgac aacgccgtga
     1381 ccacttagac aaataggatt gaataggacg cccccccatt ttgggggatt tttggctcgc
     1441 aagttattaa ttttaaacat gaaattctca ccacaatgga attatctcca aacggcacca
     1501 gggttgcttt gataagaagt aacaacatca attcaacgga aacactgagg agactttcct
     1561 cccagttgga agaggtttct atcatgcaac tttacgagct ttaccaaaac atcgtcatcg
     1621 gaacatcgat aacatgtgta atcggtctgg cggttgcatg ggtgtttttc ctgtcaaacc
     1681 gggacaggga caaataacaa gtgtgcgagt catctccttc gtctttggtc ctcccccgcg
     1741 gtcggtagcg ggaagctatc ttcatgctga acgcgtcctc ctgacaaggt attgaatgga
     1801 cgcatgataa tcgtatcgca agactctctt tctaaaacgg cctcgtcatt gcgtatgtaa
     1861 aagagggaag agtcatcatc aagaaatccg aaggaacatc catcaaattt cccaatggtt
     1921 tctcccatgc aactgtacga gttttatcaa aacattaccc tcggaacttc gataagctgt
     1981 gtggtcgggt tggcgttggc ttggattctt ttactctcca atcagaacaa atgaaaatta
     2041 taatgaacat tatagtgata gtgactccaa ttaatttcca aaagcttctt tatctgtgtg
     2101 tccccctcgc cgctgagtga cgcctgccgt ccggtccttg ggtcggacgc tctcatgaac
     2161 cggcggactt tgtgtgagcg cagcgagttg ggccgccctc ctcaggttcg cgtccgtccc
     2221 atcttgaatg agaccgggcg gggcgtcgat ggttttggat ccttttgccg aaacaaaagg
     2281 acctcggcta ccgggccgaa acccggcaac tatcttgatc gtcccaccag agagtcatcc
     2341 cctcctcttc gttctctatg gactcccccg aatgccccag cctgttgatc gtccccgccg
     2401 ttcccttccc atcgtcatcc cggcccctgc taccatagaa ttgaacttca cgctacccaa
     2461 tgttaaaaaa tagtaataac attgggggtg atcatgaaaa gctcggtaac gaaaataggc
     2521 aactcgaaag gcatcattat tccttcacat cttttgaaac agtgcgggtt tatcaaggaa
     2581 gtatctctgg aaatcaagaa tgaaactctg gtcatctcca aagcg
//
LOCUS       DATIAL010000212         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_467796, whole genome shotgun sequence.
ACCESSION   DATIAL010000212 DATIAL010000000
VERSION     DATIAL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467796"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VLA60_06615"
     CDS             complement(<1..807)
                     /locus_tag="VLA60_06615"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HSF09068.1"
                     /translation="MSDEQRNRKVIALAPMPPEKSAYALARYSRSPDSIVESLRWVHS
                     HSSEKFWEQFYFAYGHGSIADLGHATVCFEQISELAAIRLEDEPLWDGQAKSSRYQNF
                     AAAGCYVPNEIANTEIEGEYRGILGSLYNIYRLLKDPIQTHLGKRYPRPASMKPADYD
                     RTIGARTYDVIRYLLPLAAMTNVGQVVSIRTLEKQITRLLSAQLPELKGIGEDLKDAC
                     AKSPSTVWAELQQEETKGLEALVPTLARYAGANEYQSSVYRDVLRQVKGRL"
     gene            996..2360
                     /locus_tag="VLA60_06620"
     CDS             996..2360
                     /locus_tag="VLA60_06620"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HSF09069.1"
                     /translation="MNISERQSMHGQWSSRWTFILAATGAAVGLGNIWKFPYLTGQNG
                     GSAFVLVYVLCVGALGIPLMMAEILLGRRGRSTPIRSMQVLAEETNTTQWWQIVGWSG
                     TLAGMLILSYYSVIGGWTLAYIFKSGAGTFSGTSGQFAADTFNNFIGSGATLFFWHTV
                     FMILTMTVVAGGVKGGLERAIQFLMPTLFLLLLLMVAYSMTTDFFGKGLEFLFTPDFS
                     KLTGASMLTAMGQAFFSLSLGMGTIMIYGSYVPKDTSIASTSIIIGLADTLVALLAGM
                     AIFPIVFANNMEPGSGPGLIFQTLPVAFGNMPGGLAFGSLFFILLLVAAWTSSISLIE
                     PLVTWLIETFGLSRIKASLYSGFTTWLFGLGTVWSFNWWAEIKFFGLTFFDLLDFVTS
                     NLMLPLGGILIALFAGWLMKAESTKSELNIRNPTLYLAWQSLVRYIAPIAVFIVLLNA
                     IGIL"
     gene            2487..2657
                     /locus_tag="VLA60_06625"
     CDS             2487..2657
                     /locus_tag="VLA60_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09070.1"
                     /translation="MSELSALAAANFLTQASLPLIQAAVVPQPQGNPSSSSTQDTVSL
                     SLEAKALHTNHR"
     gene            3586..>4155
                     /locus_tag="VLA60_06630"
     CDS             3586..>4155
                     /locus_tag="VLA60_06630"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSF09071.1"
                     /translation="MFKGFKREQFIIEMTAVLLMFLMVGTSWGADVDARNRVSKGTLE
                     FGFLTGYWQGNNAFGNEPSANRSAVYFLPQLGLVLTDEIGSGWTSGNVEVLIEPLAAH
                     YYQPFSASAFGGSLMVKYNFLDFGRWMPFWDGGAGMLWTDLGPRIPEQSTQFNFVLQT
                     GPGVSYFVTENTAVTFGIRFHHISNAGIGE"
BASE COUNT         1004 a         1080 c          977 g         1094 t
ORIGIN      
        1 gagtcgtcct ttcacctggc gaagcacgtc cctatatacc gaactttgat attcgttagc
       61 ccccgcatac cgggctaagg tcggcaccaa ggcctctaac cctttggtct cctcttgctg
      121 caactcggcc cataccgttg agggtgattt ggcacacgca tccttcagat cttctccaat
      181 ccccttcaat tccggcaatt gagcagacag taaccgcgtg atttgctttt ccaatgtgcg
      241 aatactgacc acctgcccca cattcgtcat ggccgccagt ggaagcagat agcgaatgac
      301 atcataggtt cgagcaccga tggtccgatc ataatctgca ggcttcatac tcgccggcct
      361 gggataccgc ttccccagat gggtttggat cggatctttc aacaaccgat aaatgttata
      421 gaggctgcca aggatgccgc gatactcccc ttcgatttcc gtattggcaa tctcattggg
      481 cacataacag ccggccgccg caaagttttg ataacggctg gatttggctt gcccatccca
      541 cagaggttca tcctcaaggc gaatagcggc cagttcggag atctgttcga aacacaccgt
      601 cgcatgaccc agatcggcaa tcgagccgtg cccataggca aaataaaact gctcccaaaa
      661 tttttcggaa gaatgggaat ggacccatcg cagactctcc acaatagaat ccggtgaacg
      721 actatatcga gccagggcat aggcagattt ttccggaggc atcggggcca gagcaatcac
      781 tttccgattt cgttgttcgt cactcatcaa tgcaatcctt ccaacatatc ccgaagaaca
      841 cacaatcgaa acagaagaag acgacactat agtcagacag gagtgacatc tcaagtcaga
      901 cattatctct ctgcctggca tcacccaagg accttgaaag tgcctgacac ctacactaca
      961 ctccaccgga tttccaggta gacttccaat tctccatgaa tatttccgaa cgacaatcga
     1021 tgcatggcca atggtcctcc cgatggacct ttatccttgc ggccacaggt gcggccgtgg
     1081 ggttgggcaa tatttggaaa ttcccctacc ttacgggaca aaatggaggt agtgcgtttg
     1141 ttctggtgta tgtcctctgc gtgggagccc tggggatccc cttgatgatg gccgaaattt
     1201 tgttgggccg gagaggacgt tccacgccaa ttcgttccat gcaagtactg gcggaagaaa
     1261 ccaacaccac acaatggtgg caaattgttg gctggtccgg cactttggcg ggcatgctga
     1321 tcctttccta ctacagtgtc attgggggct ggacgctagc ctatatcttc aaatcaggag
     1381 cggggacttt cagcggaacc tccggccaat ttgctgcaga cacctttaat aattttatag
     1441 ggagcggagc cacgctcttc ttttggcaca cggtatttat gatcctgacc atgaccgtcg
     1501 tggcaggggg ggtcaaaggg ggactggaaa gagcgattca atttctcatg ccgacccttt
     1561 ttctcctttt gctgctcatg gtcgcctaca gcatgaccac ggattttttt ggcaaaggac
     1621 tggaatttct ctttacgccg gacttcagta aattgaccgg cgcgagcatg ctgacagcca
     1681 tggggcaagc cttctttagc ctcagtctcg gcatgggcac cattatgatt tacggttcgt
     1741 atgttcccaa agacacgtca atcgcttcca cctccattat cattggccta gctgataccc
     1801 ttgttgcctt gttagccggc atggccattt ttccaatcgt atttgcaaac aatatggaac
     1861 caggctccgg tccaggtctc atttttcaaa cactgcccgt cgcgttcggc aacatgcccg
     1921 gcggcctcgc cttcggttcg ctctttttca ttctcttgct tgtggcggcc tggacatcgt
     1981 cgatttcttt gatcgagccc ctggtcacat ggctcattga aacttttgga ttatcacgga
     2041 tcaaggcttc cctctattca gggttcacca cctggctgtt tggcctcgga acagtctggt
     2101 cattcaactg gtgggcagaa atcaaatttt tcggactcac cttctttgac ctgttagatt
     2161 ttgtgacatc caatctcatg ctccccctgg gagggattct cattgcactc tttgccgggt
     2221 ggctcatgaa ggccgaaagc actaaatccg aactcaacat acgcaaccct accctctacc
     2281 ttgcctggca gtccctcgta cgttacatcg cccccattgc agtgtttatt gtcctattga
     2341 acgccattgg cattttatag ccagaagacg acaacttttc ctgatcattc ccgcatgtat
     2401 gaggcaggaa tcctgaaatt ttcggccaat acaggtcaag tattccgcat actaaactga
     2461 caagacccaa ggaaaggaga atttccatga gtgaactttc tgcattagcg gccgccaact
     2521 tcctcaccca agcttcgctt ccgctcatcc aagcagccgt cgttccccaa ccccaaggaa
     2581 atccgtcatc ttcttccacc caagacacgg tatcgctttc cttggaagcc aaagccctgc
     2641 acaccaacca caggtagtct caaactaaac gctgaccaag tcaaccgtaa ttgtaagcca
     2701 ggggacagca tcgatcattt caactgccct cattaatccc tccatacttc ttgtctggta
     2761 attcagggga atctttagca agcatcgttc aactctgtca ttcatcccac cacattacca
     2821 acgcgattca atttcagaaa acgtgccatc cacttgaagc gaaaccggat cgaatccctg
     2881 tctgcagcga gggcccaaac atctattcgg caacccgcca aattcccctc tgagtttacc
     2941 tgactcaaga acctcaagac tcacaagcca tgggaattaa tctaaagcac ggctccctct
     3001 ttgtccgcgg gatcacttgc caggtttctc gggagaccgt gtgagttgca tagatttttg
     3061 ttgggaactt tcatagcctg atcacggaaa aaatgggaat gctttttttt ggtagaggaa
     3121 agaaaagaat gttaggagga ttgagcaaaa ttactgtgac taaatcgatc ggcgtcaggg
     3181 cttttggggt ggaatgcact gctcagactg gattgggtta tccatagaga gggcttgctc
     3241 tgagagatgg gccagttgat tggcccggtt gatgagttta tctaactcgc ggacggccaa
     3301 agacagcctc gcaagaggat ctttcacagg tttctttgat ggcgtgttca aagataaagg
     3361 ggaagattcc atttttttga cctcaatagt ctaaagattg taacccttta gaataaaaag
     3421 ttcattgaat tgttggttcc aattatgaga aattcctgct cattattgga atttcatgaa
     3481 atgcccgctt ggaagttgcc gataccggaa gctgaatgaa gcttaacttg caaatttccc
     3541 gtttgattca tcggaatgat ttgtagacaa ccaaaaggaa tttacatgtt caagggattc
     3601 aaaagggaac aattcatcat cgaaatgacc gccgtgctgt tgatgttcct tatggttgga
     3661 acgtcttggg gagcagatgt tgatgcgaga aatcgtgtgt ccaagggcac cctcgaattc
     3721 ggatttctca ccggatattg gcaagggaat aacgcctttg gtaatgaacc gtcagctaat
     3781 cgcagcgccg tctatttcct acctcaattg ggtctggtgt tgacggatga gattggctca
     3841 ggctggacct cgggtaatgt cgaagtgtta attgaaccgt tggctgctca ctattaccag
     3901 ccgtttagtg cctcagcgtt cggaggctcg ttaatggtga aatataattt cctcgacttt
     3961 ggacggtgga tgccgttttg ggatggtgga gcaggcatgt tgtggacgga cctggggccc
     4021 cggattcccg aacaaagcac gcaatttaat ttcgtcctcc aaacgggtcc gggtgtctcg
     4081 tattttgtga cagagaacac ggcggtgacc ttcggtattc gcttccacca tatttccaac
     4141 gcagggattg gggaa
//
LOCUS       DATIAL010000213         6238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_37884, whole genome shotgun sequence.
ACCESSION   DATIAL010000213 DATIAL010000000
VERSION     DATIAL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37884"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VLA60_06635"
     CDS             <1..310
                     /locus_tag="VLA60_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09072.1"
                     /translation="PWSYVASDTSRNRLLVLNVFGNIIAKCPNPPGAAGGITAVAYDN
                     ILDYDQISQLPTENGAWGDPGTPYHPDTLWNPAPWMMRHNIYLANELDQTIYFGYFYE
                     "
     gene            404..1204
                     /locus_tag="VLA60_06640"
     CDS             404..1204
                     /locus_tag="VLA60_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09073.1"
                     /translation="MMMKHARRRTHTIVVWAAVLCVPLGVYGQSDSNQGISIQEAGQT
                     GVLIGEDKNQDAKLFSFMNNERVQWRLSSTPFKYSRVDHGSDVPIRLVALDSVDGPPP
                     CGADGYCNLNACSADPDCPAGLPQQESESEPYEPPHDEKGIGGSVWLTTGPDGFEGGH
                     SCDFDKGTCNIKVTWSVLPDKYDKWKICWKDWGDTFTNACDVNQKIRKFENNFYVIPD
                     LKEDQHYRIRLEGRKDSNDKWKCLAKARLRNVHLNGTNIGGVVPCIVP"
     gene            1956..3185
                     /locus_tag="VLA60_06645"
     CDS             1956..3185
                     /locus_tag="VLA60_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HSF09074.1"
                     /translation="MQTSLVDSGFENEKVSVLACAPSLRHELSVADCRGLTPKLEQFL
                     AGRPETPFLALDLDQVRKKYLELQGEFPHAAIHFAVKANPAPEIIQLLATLGSRFDIA
                     SRYELDLCLSLGVPATWLSYGNTVKKEVDIAYAFGRGVRLFAFDSEAELEKLSRQAPG
                     AGVQCRVLSQGKHADWPLSRKFGCDLAMAYDLLIHSRNLGLNPLGVTFHVGSQQTDPQ
                     QWVEPLAEAAELYRQLRRQDLELEWINIGGGLPATYQTTLRPFSHYAQTIQQALQQSF
                     DATPPQLMIEPGRFLVADAGVIQSEVILVAHKSREDVRRWVYLDIGKFGGLAETMDEC
                     IKYRLRTLREGPTGPVILAGPTCDSADVLYEKTSYELPLNLAIGDRIEILSAGAYTSS
                     YASVGFNGFPPLRTYCL"
     gene            3579..4031
                     /locus_tag="VLA60_06650"
     CDS             3579..4031
                     /locus_tag="VLA60_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09075.1"
                     /translation="MSRERWSKAMWEEFLSRIFSGDHVSFIPHKIFRWDENGIYQDCF
                     FPVPCQHLLGGEHLLGRTINEVLPKDSARSLKKALIKTLKTQRPQDVQLVFPTLGKTV
                     VAVVRLFPYESEALGFVTDHYLDGCPVISVSPQDPSLSFLKSSSPYTM"
     gene            4195..4686
                     /locus_tag="VLA60_06655"
     CDS             4195..4686
                     /locus_tag="VLA60_06655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HSF09076.1"
                     /translation="MVWKVRQDPTRWKGWGFRTDNLRQAFMWPSVVFAGSGLAMAWYG
                     IVAGQGLWQGHMLLLLFLYPLWGVLQQFLVQALGVDNLMKIFPQHGLWLAMPIGVILF
                     ALVHFPDWWLMLATGLLACFFIPFYIRDRNLWPLGLYHGWLGTFFYLWVLGRDPWVSV
                     FGR"
     gene            4764..5048
                     /locus_tag="VLA60_06660"
     CDS             4764..5048
                     /locus_tag="VLA60_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09077.1"
                     /translation="MENNPLVISDQERLQLLYERFRDVCLVEKEVWTEIYMPRQTEKG
                     MVLTNQHECYEVIIDDEAVENALEANIPLGSKALGAAIQEYKDHISFRKK"
     gene            complement(5315..5731)
                     /locus_tag="VLA60_06665"
     CDS             complement(5315..5731)
                     /locus_tag="VLA60_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09078.1"
                     /translation="MGTEITRYAIGDFGKLHKFTGKNVPAGTEALVEEDGKSLTAKGK
                     TYTARFSDILPNVEIIRVQFDQYSSNHIGFAMEAHDKTLTKIPEMLETTKIISEKLRH
                     DWMAQDKAYQDTEKAKKVAPAAAKVTAPAKEAIAAT"
     gene            complement(5974..>6238)
                     /locus_tag="VLA60_06670"
     CDS             complement(5974..>6238)
                     /locus_tag="VLA60_06670"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HSF09079.1"
                     /translation="SREAEDQADEQGFRLLQRSGINPDGMITFFAKLGKEQPPNDVIR
                     YLSTHPATQDRLAHLKSLLPPASMTYRTFPFHAEWSDINTYCK"
BASE COUNT         1445 a         1368 c         1664 g         1761 t
ORIGIN      
        1 gccctggtcg tatgtcgcca gtgataccag tcgaaataga ctcctcgtct tgaacgtatt
       61 tggaaatatc atcgcgaaat gtccgaatcc tcctggcgca gccggcggga ttaccgcagt
      121 ggcgtatgac aatatcctgg actatgacca aatctcacag ttgccgacag aaaacggtgc
      181 gtggggtgat ccgggaaccc catatcatcc agacaccctt tggaatcctg caccgtggat
      241 gatgcggcat aacatctatc tggccaatga attggaccag acgatttatt ttggatattt
      301 ctacgagtaa ggccatgaga caactcggag taacattctc acccttaacg atgttctgca
      361 atgcagaaag ggggcagtca tgaaaaattt caccaggagg tccatgatga tgaaacatgc
      421 aagacgtcga actcatacca tcgttgtatg ggctgcagtg ctctgcgtcc cacttggtgt
      481 gtatggccag tctgatagta atcagggaat atccattcaa gaggccggtc aaacaggagt
      541 attgattgga gaagataaga atcaggacgc taagcttttt tcgttcatga ataatgaaag
      601 ggttcagtgg cgtctgtctt ccactccatt caaatattca cgagtagatc atgggagtga
      661 tgttccgata cggcttgtgg cgcttgattc tgttgatggg ccaccccctt gtggggcgga
      721 tggctattgt aacttgaacg cttgctctgc cgacccggat tgtcccgccg gccttcccca
      781 gcaggagagt gaaagcgagc cttacgaacc tccgcatgat gaaaaaggca tcggggggtc
      841 tgtttggctg acgacaggtc ctgacggatt tgagggtggc cattcctgtg attttgacaa
      901 aggcacttgt aacatcaaag tgacctggag tgtgctgcca gataaatatg ataaatggaa
      961 aatctgctgg aaagattggg gcgatacctt taccaacgcg tgtgacgtga accaaaaaat
     1021 tcgaaaattt gaaaacaatt tttatgtcat tcctgacttg aaagaagatc aacactatcg
     1081 tattcggctc gaaggtcgaa aggattcaaa cgataagtgg aaatgcctgg ccaaggcgcg
     1141 attgcggaat gttcacttga atgggacgaa tataggagga gttgtgccat gtatcgtgcc
     1201 gtaattttgc cattggacag gtacggtctt gcttcgggaa aattttgggg catgaagccc
     1261 gctagaacta ttttactgta ccaacgcgtc tacggacttt agagagaaag gtagattctc
     1321 tcgtttatcc gataagaact cagagagggt atatggcccg gttcgaaaag ttttttccag
     1381 gaaggaggga ggaggcattc acagatggaa aagctcgtgg tatgctttct tcgatcatat
     1441 tgaagggcaa atttcagatt gaaacgtggt gtcgttaggg ttttttatag aataagctct
     1501 tggctgaaaa tttgtctcgt gacccctgcc tatagaataa ggaacttcca tcccgcgaac
     1561 gtgtgttctc tttcatggcc gagctgaaac ccgggaatcg ggtttcactc atcgattcct
     1621 gaaacgagct attgctcgcc tctttcccac attagttgtg tccctttcga cgcatcctcc
     1681 tggtcccgct aaacaacact tcaggcatta ttggtgtatc aagaccttgg atttcccacg
     1741 gtacgttacc aagatttctc aaacgaatat cccgtctgcc atcagaccgg aaaaactttg
     1801 cgcccgggat caaggagtct ggttttgggc atgtgaatgg ttccggaatt ctcttaagat
     1861 tttctggata ttttgtcgat gtagttgatg gttttttgac tttcggttcg gcgactttaa
     1921 ttcccattta atcaccattg tcacaggagt agaatgtgca gaccagtttg gttgattccg
     1981 ggttcgaaaa tgagaaagtt tctgttctgg catgtgctcc atcgcttcgc catgagttgt
     2041 cggtggctga ttgtcgtggt ctgaccccaa aactcgagca gttcctggcc ggtcgtcctg
     2101 agacaccgtt tttagccctg gaccttgatc aggtccgaaa gaagtatcta gagcttcaag
     2161 gagaatttcc tcatgcagcg attcattttg cggtgaaagc caatcctgcc ccggaaatta
     2221 ttcaactgtt agccacgttg ggctcacgat ttgatattgc aagccggtat gaactggatt
     2281 tgtgtttgtc tctcggggtt ccggccacct ggctgtcgta tggcaacacg gttaaaaaag
     2341 aggtggatat tgcgtatgcc tttggccgcg gggtcaggct cttcgccttt gacagtgagg
     2401 ccgaactcga aaaattgagt cgacaggcgc ctggtgcggg agtgcagtgt cgagtcctga
     2461 gccaagggaa acacgcggat tggcctcttt ctagaaaatt cggctgtgac ttggccatgg
     2521 cgtatgattt gctcatccac agtcgtaacc tcgggttgaa ccccctggga gtgactttcc
     2581 atgtggggtc tcaacaaacc gatccgcagc aatgggtcga gcctcttgcg gaggctgcgg
     2641 aattgtaccg ccaattgagg agacaggatc ttgagctgga atggatcaat attggcggag
     2701 gacttccggc tacctaccaa acgacccttc ggccattttc gcactacgcc caaaccattc
     2761 aacaggctct ccagcaatca tttgatgcca cacctcctca actgatgatt gaacccggtc
     2821 gatttctggt ggcggatgcg ggagtgatcc agagcgaggt tatcctggtg gctcacaaat
     2881 cccgagaaga tgtccggcgg tgggtgtatc ttgatattgg taagtttggc ggtttggctg
     2941 aaacgatgga tgaatgtatt aaatatcgcc tacgcaccct ccgtgagggt ccaaccggac
     3001 cggtgatttt ggcaggtcct acttgcgaca gcgcggacgt gttatatgaa aaaacctctt
     3061 atgagttgcc cctgaatttg gctatcggtg atcgaatcga aattttaagt gccggtgcct
     3121 ataccagcag ttacgcgtct gtggggttta atggatttcc tcccttgcgg acctactgtc
     3181 tctaacggat agcccggtta gtgtaatggg cttattccat tggtcattgt ggtacgcagg
     3241 ccgttgattt atttcccttg gttttctaca gtcttgtacg aatttagata gcgctgcagg
     3301 gcggtttcgt cgatatcaat ggctgaaaag cctggaccac gcccttctcg gtgaatgaag
     3361 aaggctgcct aagtttcagg caaggaggaa agggtaaatt gtattaattg ttcctttttt
     3421 aatacatata gaatgctagg atcttttttt cgaataacta gggatttcac tagttgtgca
     3481 tgaagtggaa agtcttattt actctttata aagagcactg aaatattcgt taggagaatc
     3541 atctctatga taggggggat agaaaccagg gaggggccat gtccagggaa cgttggtcga
     3601 aggccatgtg ggaagaattc ctgagcagga ttttttcagg ggatcatgtc tcgtttattc
     3661 cccataaaat ctttcgctgg gatgaaaatg gaatctatca ggattgtttt ttccctgttc
     3721 cctgtcaaca ccttttaggt ggagagcatc ttcttggacg gactatcaat gaggtcttac
     3781 cgaaagactc agccagaagt ttgaaaaagg ccctgatcaa aactctgaaa acccaacggc
     3841 cgcaagatgt tcaattggtt tttccaaccc tgggaaagac ggttgtcgcg gtggtccggt
     3901 tatttcctta tgagtctgag gcattgggct tcgtaacaga tcattacctc gatggttgtc
     3961 ccgtcatctc ggtttccccg caggatccct ccttgtcctt cctgaaaagc tcctcgcctt
     4021 ataccatgta attcgtgtgg gagaaatctt ctgaacattt ctcccgatga tcgggctggc
     4081 aattggtggg aattctctgc ggttgtcctg acagggctgc tgcatcttgt attcaagagt
     4141 cttgatgcaa agggactctt tatagccttg gccggcatca gttggatgag ctatgtggtc
     4201 tggaaggttc ggcaagatcc gacgaggtgg aaaggttggg gatttcgcac cgacaatctt
     4261 cgacaagcct ttatgtggcc atcggtagta tttgccgggt cgggattagc catggcctgg
     4321 tatggcatag tagccggtca agggctctgg caaggtcaca tgctgttgct gttgttcctg
     4381 tatcctttgt ggggagtcct ccaacagttt cttgtgcagg cgttgggtgt cgataatctc
     4441 atgaaaatct ttcctcagca tggcctgtgg ctggctatgc ccattggggt gattctgttt
     4501 gcccttgtcc atttccccga ctggtggctg atgctggcta ccggcctttt ggcctgcttt
     4561 tttattccgt tctatatccg tgatcggaat ctttggccac tggggctcta tcatggctgg
     4621 ctgggcacgt ttttttatct ctgggttctt ggccgcgatc cctgggtatc cgttttcgga
     4681 agatagccac ccacttgacg agtttgatga gagcggagta gaaaaagaac cattgttgtc
     4741 gatgtgaagg gagaggtatg gctatggaaa ataatccgct cgtaatttct gatcaagaac
     4801 ggttgcagct tttgtatgaa cggtttcggg atgtgtgttt ggtggaaaag gaagtgtgga
     4861 cggaaatcta tatgccccgg caaacggaga agggcatggt gctcaccaat caacacgaat
     4921 gttatgaagt gatcattgat gatgaagcgg ttgaaaatgc actggaggcc aatattccat
     4981 taggttcgaa ggcgttgggc gcggcgattc aggagtataa agaccatatt tcgtttcgaa
     5041 aaaaatagtt cgtatagtcg agcagggtgg ataaaagcct gatggttacc gtcatgacta
     5101 ttgccagctc ttctccagcc tggctatcaa ggttctgcag ggtgcaaccg gcttaacccg
     5161 gccctcattc ggatttcttt agttcgagga ttgaaaaata ttgaagcttt tccccctctg
     5221 cttttttggg cagaggggga acaatggaga tgaccctgaa gcgaacggcg tcttttatta
     5281 gccctgacac gttctttgat tggggagggg ttaattacgt tgctgcaatc gcttcttttg
     5341 cgggcgctgt gacttttgcg gcagcaggcg ctaccttttt ggctttttcg gtatcctgat
     5401 aggctttgtc ttgcgccatc caatcatgtc tgagtttttc agaaatgatt tttgtggttt
     5461 ccagcatttc cgggattttg gtcagggttt tgtcatgggc ttccatggcg aagccgatgt
     5521 ggttcgagct gtattgatcg aattgcacgc gaatgatttc cacgttcgga agaatatccg
     5581 aaaaccgtgc ggtataggtt ttccccttcg ccgttaagct ttttccgtct tcttcgacca
     5641 gggcctcggt gcctgccggt acattctttc cggtaaattt atgcaacttt ccaaaatctc
     5701 caatggcata gcgtgttatt tcggttccca tgtgacccac cccttgttgt gtgttgatga
     5761 taagagcctc ggactagaca gacggggtgt gagtgtattt ggcattgtgg tccagacaaa
     5821 gacaccatac attccggtat gtctcgaata ggtttattat gaagcctcca gggctagaga
     5881 gcaatgggtc agatttccta aaaggtgagt ggaacctccc ctcaacagcc ctcccgcagg
     5941 agtcgaccag cgttaagtag aggccctatg gggtcacttg cagtatgtgt tgatgtcaga
     6001 ccactcagcg tgaaacggga aggttcgata ggtcatggag gctggtggca gcaaggactt
     6061 caggtgtgcc aggcggtctt gggtcgctgg atgggtcgaa agataacgaa tgacgtcgtt
     6121 tgggggttgt tctttcccaa gtttagcgaa aaatgtgatc atcccatcgg gattaatccc
     6181 cgaccgttgt aataaccgga atccctgttc atcggcttgg tcttccgcct cacggcta
//
LOCUS       DATIAL010000214         5793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1328414, whole genome shotgun sequence.
ACCESSION   DATIAL010000214 DATIAL010000000
VERSION     DATIAL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328414"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VLA60_06675"
     CDS             complement(<1..301)
                     /locus_tag="VLA60_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HSF09080.1"
                     /translation="MDPKDLMAMNATMGSRGPDGVGLYVQGRMGFGHRRLKVIDLTQA
                     SQQPMVDSALGLGIVFNGAIYNYKELRHELEEKGYAFFSQGDTEVILKAYHAWGEG"
     gene            993..1397
                     /locus_tag="VLA60_06680"
     CDS             993..1397
                     /locus_tag="VLA60_06680"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HSF09081.1"
                     /translation="MNITQIRIDPDPYEPYRLSQGYRVGDLLFISGQTAIDTNGRIVG
                     AGNFDVQANQTFDNLERVLHAGGSSLGNVIKVTIFLTDMTHFDKIVELRGQRFTAPYP
                     ADTIVEVRSLYSPDAMIEIEAIAVADSSAERK"
     gene            1818..2267
                     /locus_tag="VLA60_06685"
     CDS             1818..2267
                     /locus_tag="VLA60_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSF09082.1"
                     /translation="MSEHKITLEWKRESENFSYESYNRDHELVFEGGVRVPASAAPAY
                     RGNPAHVNPEEAFVAALSSCHMLTFLAMAAKKRFVVDRYSDHAVGLLEKNQNKKLAIT
                     RVILHPRVIFGGPTLPTQEQIHALHEHAHSECFIANSVTTEVTVEAE"
     gene            2378..3637
                     /locus_tag="VLA60_06690"
     CDS             2378..3637
                     /locus_tag="VLA60_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSF09083.1"
                     /translation="MSERPAKQVREVVEEVYRTESRRILATLIRLLGDFDAAEEALHE
                     AFAVAVEQWTRDGIPANPRAWLVSTGRFKAIDGMRRRTRFDASLAELATQWEAGIHDA
                     HENPDESIDDDRLRLIFTCCHPALSSEAQVAMTLREVCGLTTEEIAKAFLTSPPTVAQ
                     RIVRAKAKIRKARIPYEVPSETILPDRLDAVLRVIYLVFNEGYSASSGESLTRHDLSA
                     EAIRLGRLLMELLPDPEAMGLLALMLLHDSRRAARISRTGDLILLEDQDRSLWNRDQI
                     AEGIALVGKAFASRQIGPYTIQAAISGLHSQATHPTATDWPQIVSLYDLLMQASPSPV
                     VELNRAVAVAMRDNPLAGLALIDAIFARGDLTNYYLAHSAKAELCRRLGRIAEAKAAY
                     QQALGLTQQKPEQRFLEQRLTGLQDRN"
     gene            3854..4240
                     /locus_tag="VLA60_06695"
     CDS             3854..4240
                     /locus_tag="VLA60_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSF09084.1"
                     /translation="MKYLLFCCHEEKNFDSMTKSEGDALMNETLTYCEKLKRSGHLIV
                     AEPLEPIQMAVTVRVRNGGVSLTDGPFAETKEQIGGFFLIEARDINQAVQIASKFPSV
                     RLGSMEVRPVRDLSRQGIEAESCQDE"
     gene            4318..4689
                     /locus_tag="VLA60_06700"
     CDS             4318..4689
                     /locus_tag="VLA60_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSF09085.1"
                     /translation="MKYLLLIHHDEDAFSAMSETIRKEMLEESVQLTHQLHTKGQYLS
                     ASPLHPASTAVLVRVREGKPLVTDGPFVETREQLAGYFLINAEDLDEAIGIALRVPGA
                     RIGTVEVRPLIEISGLPTEKC"
     gene            4887..5312
                     /locus_tag="VLA60_06705"
     CDS             4887..5312
                     /locus_tag="VLA60_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSF09086.1"
                     /translation="MRFMIIVKATKDSEAGVMPSEQLLTEMGKFNEELIKAGVMLAGD
                     GLHPSSKGVRVQFSGNKRTVIDGPFAETKELIAGYWVWQVNSKEEAIEWVKRCPNPGG
                     DDKEGEIEIRQLFEAEDFGPEFTPELREQEEHLRKNLSS"
     gene            5392..>5793
                     /locus_tag="VLA60_06710"
     CDS             5392..>5793
                     /locus_tag="VLA60_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HSF09087.1"
                     /translation="MRLTTITFTCLCMVLTAIPVIATDTDKEKQMDPQAMMEMYKKLA
                     APGEPHKLFASLAGSWTTKSKEWMEPGKPPTESTGTAEMKMLLDGRYLYQAYTGQMMG
                     QPFSGIGIDAYDNITKKYVTAWMDTMGTGIFI"
BASE COUNT         1511 a         1475 c         1464 g         1343 t
ORIGIN      
        1 agccttctcc ccacgcatgg taggccttga gaatgacttc agtatctccc tgggaaaaga
       61 aggcatagcc tttctcttcc agctcatgcc gcaattcctt gtagttgtat atcgccccat
      121 taaatacaat gccaagcccc aaggcggaat ccaccatggg ttgctgggac gcctgggtca
      181 agtcaatgac tttgaggcgt cgatgcccga atcccattcg cccttgaaca tacagtccca
      241 ctccatcagg gccacgactg cccatggtcg cattcatggc catcaaatct ttggggtcca
      301 caggacaccc tgagaaattt atctgtccaa gaattccgca catttgctca tcaacctttc
      361 aatcatcttg aaatgctttt atcgcacatg tgattgttgt cgatcacaga taaatacaag
      421 catccctggc tcatcccctt tgagaaggaa atcttcacca gggataattt ttttaagaat
      481 tggcccatta tgaacgggaa taatgtccag aggatgggaa aacttaccct aataattaat
      541 ctcggctttg tcaaacaaat ccaatggata tgcactcacc agaggccgga aaatactcta
      601 tgcgaaaggg gtcttctgcg tgatattttc aatcatgtaa ggaagcatag ttgcgaggct
      661 cccgatagaa ctcttttgaa cagagatttt tgtcgcacac atacctagca ggctgtcgga
      721 aaactcaaat attcttctga gaacttatcg ggttcaaaaa ttttacgatg ttcaacgtag
      781 tcaagcagga tattcaagaa ggcccgtaat gcgagtcggt ttagaaattc tggcttggcc
      841 ttcaacagtg agacgattcg aggcaccgat accaagccaa atgaatagcc tccgattaga
      901 tatactgaat catcttcgtg tgtcattaat gggcaatcgc agcggtaagg cgtttttgag
      961 gtctttttcg acagcaataa ggaggtggta cgatgaacat cacacaaatc cggatcgatc
     1021 cggatcccta cgagccctat cggctttccc aggggtatcg ggtcggtgat ctgctcttta
     1081 tctcaggtca gactgccatc gatacgaacg gtcgaatcgt cggcgcaggc aactttgatg
     1141 tccaggccaa ccaaactttt gataatctcg agcgggtact gcatgcggga ggatcaagcc
     1201 tagggaacgt catcaaggtg acgatcttcc tgaccgacat gacccatttc gataaaatcg
     1261 ttgaacttcg cggtcaacgg tttacggctc catatccggc agacaccatc gtggaagtcc
     1321 gctcactata ttctcccgac gcgatgattg agattgaagc tattgccgtt gccgactctt
     1381 cggctgagcg gaagtaagag tcgttcgggt aaaataaact atgaatgaaa tcttgaaatt
     1441 agagccggcg gcccgcttgg aagaatccga ccagcttctt atggaacccc caggcgccgg
     1501 tacaatggaa atattgtctg gtggacactt tcttcagacg aaccgccaca tattgagatg
     1561 cacaaattac acatcaataa aaattgctct gatgaggaaa aatacacgag tacggaaaac
     1621 agcggcaagc actgcgaaat gaagagtgta ggttgatgcg acgttattat gctgtttttt
     1681 gaagatccca gcggcaatca cctggaaacc tgttaccgcg tatgcgcata agtacccgga
     1741 ttcgttttcg atcgtcacgc acagggaagt cgcctcaata aaaaaataat tgtcacgttt
     1801 tcgaaagggg ttgatcaatg tctgagcata agatcacgct agaatggaag agggaatcag
     1861 agaacttttc gtacgaatcg tataatcgag accatgagtt ggtttttgaa ggtggggttc
     1921 gcgttcccgc ctccgccgca ccggcctatc ggggaaatcc cgctcatgtg aatcctgagg
     1981 aagccttcgt ggcagccttg tcgagctgtc atatgctgac tttcctggcg atggcagcca
     2041 agaaacgatt cgttgtggat cgctatagtg atcatgctgt tggattgtta gaaaaaaatc
     2101 agaacaaaaa gctggctatt acacgtgtca tcttacatcc tcgagtgata ttcggtgggc
     2161 cgactcttcc gactcaggaa cagatacacg cactccatga gcacgctcac agcgaatgtt
     2221 ttattgccaa ttcggtgaca acagaggtca cggttgaagc agaataacgt acaacatcct
     2281 ctctcattcc acctcagcag aggcgatcgc ttccctgaac gcgcgaccca taccccggtg
     2341 gtctttccgt gcctctcaac gtgagacctt cccgatcatg agtgaaagac cggccaaaca
     2401 ggtgcgggag gtggtggaag aagtgtaccg cactgaatcg cgccggattc tcgccacctt
     2461 gatccgcctg ctcggcgact ttgatgcggc tgaagaagcg ttgcacgagg cgtttgcggt
     2521 cgcagtcgaa caatggacca gagatgggat cccggccaat cctcgagcct ggctcgtgtc
     2581 gaccggccga ttcaaagcga tcgatgggat gcggcgccgg accaggtttg acgcctcgct
     2641 tgcagaactc gccacgcagt gggaagccgg cattcatgac gctcatgaaa atccggatga
     2701 aagcatcgac gacgaccggc tacgtctgat ctttacttgc tgccacccgg ctctttcatc
     2761 tgaagcccaa gtagcaatga ctctccggga ggtttgtggc ctaacgacgg aagaaatcgc
     2821 gaaagccttt ctcacatccc cgcccaccgt ggctcagcgg atcgttcgcg ccaaggcaaa
     2881 gattcgaaaa gcacgcatac cctatgaggt gccttctgag accatcttgc ccgaccgtct
     2941 ggatgcggtg ctgcgggtga tttacctcgt gtttaacgaa ggatactctg cctcttcagg
     3001 ggaatctctc acacgtcatg acctctcagc tgaagctatt cgactggggc gattattgat
     3061 ggaattactg ccggacccgg aagcgatggg gctcttggca ttaatgctcc tccatgactc
     3121 ccgccgcgcc gcacgcatct cccgaacggg agatctgatt ctgcttgaag accaggatcg
     3181 ttcgttgtgg aaccgggatc agatcgcaga ggggattgcc ctggtaggaa aggcgtttgc
     3241 ttcgcgtcaa atcggccctt ataccatcca ggccgcgatt tccggattac attcccaggc
     3301 aacacatccc acagccacgg actggcctca aatcgtgtca ctctacgatc tgttgatgca
     3361 ggcgtctcct tcccctgtgg tggaactgaa tcgcgccgtc gcagtggcca tgcgtgacaa
     3421 tcccctggca ggtcttgccc tcatcgacgc cattttcgca cgaggggatc tcacgaatta
     3481 ttacctagca cattcggcga aggcggagct ctgccgacga ttgggccgaa ttgcggaagc
     3541 caaggccgca taccaacagg ctctcggcct cactcagcag aagcctgagc agcgatttct
     3601 tgaacaacgg ctgaccgggt tacaggatcg caattaatcc ccctccctat ctgtcccaat
     3661 aggtagtttt tccgattttt agtgaccaga tcaatggtct cccacaatat cttgttgaga
     3721 attttttgaa gctctctatt cctagagtgc aatggtgccg ccggaattca tgatgaccgt
     3781 tgtcgaattt gatcttcctg aacgactaac tgacaacaag accaattcac cacctattcg
     3841 aaggccggaa gttatgaaat atcttctctt ttgttgccac gaggaaaaga acttcgacag
     3901 catgaccaaa agcgagggcg atgctctcat gaacgaaacg ttgacctact gcgagaaact
     3961 caagagaagc ggccacctca ttgtcgcgga accccttgag ccaatccaga tggcggtaac
     4021 cgtgcgggtc cgcaacggcg gagtgtctct caccgatggc cctttcgccg aaacaaagga
     4081 acagatcggc ggattttttc taatcgaggc cagagacatt aaccaggctg ttcagatcgc
     4141 ttccaagttt ccatcagtgc gcctcgggag tatggaggtt cggccggtga gggacctgtc
     4201 ccggcaaggg atagaagcgg aatcttgtca ggatgagtag atttttgtga ggaacaataa
     4261 gatcggggaa aatccatacg gtcattccta gcataaccac ctgatagagg cattcacatg
     4321 aaatacctgt tgttgatcca tcatgacgaa gacgcattca gcgcaatgag tgagaccatc
     4381 cgcaaggaga tgcttgagga atccgtgcaa ctcacacatc agctccacac caagggacag
     4441 tatctgagtg cgtcgccgct tcatccggca tcgacggctg tcctcgttcg tgtgcgcgaa
     4501 ggcaagcctt tggtgactga cggtcctttc gtggaaacac gcgagcaact ggcgggatac
     4561 tttttgatca atgccgagga tcttgacgag gcgatcggta tcgcattgcg ggttccaggg
     4621 gcgcgcatag gcaccgtcga ggtgcgaccg ttgatcgaga tctccggtct gccgactgag
     4681 aaatgttgat gcgcagaaaa gaatccgctt caatcctgtc caagctgcct tgtggctcta
     4741 tgattagaaa cacatggtgg cagagctgcg actacacagt tttttcacat gtcgatttca
     4801 ccagcccccg ttcgacaaat aaggaaaata cggataaggc tattttttaa ttcagttcaa
     4861 tgtactaaag ccaaggagat tgggtgatga gattcatgat tattgtcaaa gccaccaaag
     4921 actcggaagc cggcgtgatg cctagcgagc aacttcttac tgagatggga aaattcaacg
     4981 aagaactgat aaaagccggc gtcatgctgg caggagatgg gctccacccg agttcaaaag
     5041 gcgtgagggt gcagttttca ggaaacaagc gaacagtgat cgacggaccc tttgccgaga
     5101 ccaaagaact gatcgccggc tattgggtct ggcaggtgaa ttcgaaagaa gaggcgatcg
     5161 aatgggtcaa gcgttgtccg aatcctgggg gtgatgacaa agaaggcgag attgagattc
     5221 gccaactttt cgaggcagag gacttcggac cagagttcac acctgagctg agggagcaag
     5281 aggagcacct gcgcaaaaat ttgtcttcat gaaagtaccc tgtgaacagt cactcacagc
     5341 atgacatact gatctcaact gttttcatta tctaatcaca aggaggcatc tatgcgtttg
     5401 acaaccatca cattcacctg tctctgtatg gtcctgacgg caattccagt catcgccaca
     5461 gacacagaca aagaaaagca gatggaccca caagccatga tggagatgta caagaaactg
     5521 gccgctcccg gtgaaccgca caagttgttt gccagccttg ccgggagctg gacaaccaaa
     5581 tccaaagaat ggatggaacc aggcaaacct cccacagaat cgaccggcac cgcagaaatg
     5641 aaaatgttgc tggacggacg ctatctctac caagcctaca ctggccaaat gatgggacaa
     5701 ccattctccg ggattggaat cgacgcctac gacaacatca ccaagaaata tgtcacggcc
     5761 tggatggata cgatggggac ggggattttc atc
//
LOCUS       DATIAL010000215         6705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1436567, whole genome shotgun sequence.
ACCESSION   DATIAL010000215 DATIAL010000000
VERSION     DATIAL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436567"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1267
                     /locus_tag="VLA60_06715"
     CDS             272..1267
                     /locus_tag="VLA60_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HSF09088.1"
                     /translation="MSPHQSYPAERYLTGIGEAESRAQAEKMAYAAVARVFSAQVNTQ
                     SMDHETYSIQESGGGSQTRRELQLDQRTQVTTSKALENVQILDLWYQPSTRHFWALAG
                     LDRQQAEKAILERLQEWDTKIENMIHQGRTHPQKIQRIRGYKEAMILLADRDALNTDL
                     RVIRTSGDSLPPSYRIPQIQREFMDFVAQNLVISVAIEGEYHEDIERAILEGLKQEGL
                     LGRPADSKSSEVADLAIVGQGQFWTVNLPDPLFKYVRWCGDIDIYENPSHRLIGVISE
                     TGREGHVTEKEARIRASKVMQEVISKEIARLLTRSVFSVEQDPSLSQRVPNACSH"
     gene            complement(1358..1831)
                     /locus_tag="VLA60_06720"
     CDS             complement(1358..1831)
                     /locus_tag="VLA60_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09089.1"
                     /translation="MVLMVGYAERGSTQMIGELGEMDRLEQHAEDAIANGDPEGAALS
                     IGKAALMAKILAQKEEHQQTRALYQAAETLFRGQEQGYRAFALFERAGGQPPASKGVC
                     QYLAQADEKVKQSQKELRALPDFTNESLRDRQHRHIGKTQEWERLLQSLEHDLSC"
     gene            complement(1947..3050)
                     /locus_tag="VLA60_06725"
     CDS             complement(1947..3050)
                     /locus_tag="VLA60_06725"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF09090.1"
                     /translation="MRTWAACGFISIVLLVGFFGSDGFARRTYLDQEQKAQLEGIQTI
                     MIHVLALTERGKVDGTVIEKVVRQRLEELGYTVVTDRDAPFDVRVNVKCEEVKKWSGT
                     TRGGGDADHSGAPSRLWKGPACLFSYQLDGRDLGWYKEARTSFEDPGEAAKVAHAPQS
                     GLYAIQQLAERIQEFDFPVLLAAEWGHDARLFKLLNNPQTPNKRKLRILSLLPQVESH
                     DTLPLLKELIQDPELAEEAIVALSSTGSEGIPLLTDIFQTQKDSKIRAAAAKGLGEIG
                     AHTGDPSVTPPMLGYVVKNLPHIKSSSDIDFPVLTEVVWSLGKLRNEKSIAPINELQS
                     KIWVIFDNSKEMAELREATSWTYKQIDMDWQVQ"
     gene            complement(3084..4178)
                     /locus_tag="VLA60_06730"
     CDS             complement(3084..4178)
                     /locus_tag="VLA60_06730"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF09091.1"
                     /translation="MTRWPVFLIFAILCVGVMASEARRIHLTPEQKTQLENAQTVLVE
                     VLALTEKGTYDPSPLVATIKARLEDMGYTVTTDRSQPHDVEVKVKCEEQKTVTGTSPT
                     GGDVDLADAPNRLWKGPACLLTYFLQHNDLQWKKEVRTTFADARQAAKQAQIDDAGTY
                     AMDQLNQRLSEYDFPVLLAAEWGQIDRLLKLLDNPNTPKLRKVKILSVLSDVHAEEAL
                     PQLTKLLESTDLQQETINALSGAGGHSIPLLIDLFQTSRQSSIRAEAAKALGDIAAKT
                     GDPRPIPPLVQYVSALLPQLKTSEDIDFPVLTEVVWAIGKQRWEGSLKPMRELQQKIW
                     VIFDNSEEMAELREATSWTYKQIDLDGHLS"
     gene            complement(4285..5394)
                     /locus_tag="VLA60_06735"
     CDS             complement(4285..5394)
                     /locus_tag="VLA60_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF09092.1"
                     /translation="MKAWPVFFIFVILGVGGMVSEARQIPLTPEQKTQLENAQTVLVE
                     VLALTEKGSFDPSPLVATVKARLEDMGYTVTTDRSQPHDVAVKVKCEEAKTSTRISSS
                     GGDVDLADAPNRLWKGPACLLTYFLKQNNFQWKKEVRTTFPDARQAAQQAQVDDAGAY
                     AMDQLNLRLAEYDFPVLLAAEWGQIDRLLKLLDNPNTPKLRKVKILSVLSDVHAEEAL
                     PQLTKLLESTDLQQETINALSGAGADSIPVLIDLFQTSPQSNMRAEAAKALGDIAART
                     GDPRTIPPLVEYVSEVLPQLKTSEDIDFPVLTEVVWAIGKLRWAHSLKPIAELQDKVW
                     LIHDNSKEMAELREATNWTYKAIELQDAAMLPSYC"
     gene            5748..6560
                     /gene="proC"
                     /locus_tag="VLA60_06740"
     CDS             5748..6560
                     /gene="proC"
                     /locus_tag="VLA60_06740"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HSF09093.1"
                     /translation="MGQSPKFVFLGAGNMADALVTGILKAHLASPANISVTDISPIRL
                     EHFQKTFQVHVGSDNADEVKSGDIIVLCVKPQVMDTVLAEIKEQVSQKHLLISVAAGY
                     PLARIQQHVGRNISLIRAMPNTPAVIQEGVTALAGSSGISADHLQLAQSIFESVGKVV
                     MVEESLMDAVTGLSGSGPAYIYLVIEALTDGGVLVGLPRTVASVLATQTVLGAARMVL
                     ESGEHPAVLKDRVTSPGGTTIAGLQQLEAGKLRATLMKAVKAATARSYELGQ"
BASE COUNT         1572 a         1631 c         1749 g         1753 t
ORIGIN      
        1 aactcttcca ggtgagcttc agttgacacg actctgggtt gaaccgggtg actattccgt
       61 ggctatccag tccatggatt accagggacg gaaggctggg gagtttcaac atcatcggct
      121 tcaacttatg aaaggcgaaa caacgcttgt cattcaccag agtttccaat aagtaatcat
      181 gaatatcctg agaatattgc tatggagtgg aatatgcatc ctagccggag gctgtgcctg
      241 gtttggcagg gagaccccgc ctgaatggat catgtcgcct caccaatcat accccgctga
      301 acgatatctc accggtattg gagaagccga atcccgggcg caagctgaaa aaatggcgta
      361 tgccgccgtt gccagagtat tttctgcgca ggtgaacacc caatcgatgg accatgaaac
      421 gtattccatt caagagtccg ggggggggag tcaaactcga cgggagttac aactcgacca
      481 acggacacag gtgacaacca gcaaagccct ggaaaatgtg cagatattgg acctctggta
      541 tcagccgtct accaggcatt tttgggcttt ggcgggcctc gaccggcaac aagccgagaa
      601 agccattctg gaacgattac aggaatggga tacgaagatt gagaatatga ttcatcaggg
      661 ccgaacacat cctcagaaaa ttcaacgcat ccgaggctac aaagaagcca tgattttatt
      721 agctgatcgg gatgccctca atacagatct ccgggtgatc cgcaccagtg gagacagtct
      781 gccaccgtcc tatcgtatcc cacagatcca acgtgaattc atggattttg ttgcccaaaa
      841 tttggttatc tcggtggcga ttgagggaga ataccatgag gacattgaac gggcgatctt
      901 ggaggggctc aagcaggaag gcttattagg ccgtcccgcc gattcaaagt cctcggaggt
      961 tgcggacctg gctattgttg ggcaaggaca attttggacc gtgaatttac cggatccctt
     1021 gttcaaatat gtgcgatggt gtggcgatat cgatatctat gaaaatcctt ctcatcgact
     1081 tatcggggtg atttcggaga cggggcggga agggcatgtg accgaaaagg aagcacggat
     1141 tcgtgccagc aaagtcatgc aggaagtcat ctccaaagaa atcgcccgtc tgctcacacg
     1201 atccgtgttc agtgtcgaac aggatccgtc actgagtcag agagttccca acgcctgttc
     1261 tcactaacac acggtcttcc tggtctcttt aaagacgtct ctttttcttt gtgaggacgg
     1321 gaaataaatt tggtgagtca gataaattga gaaaaggcta acacgacaag tcatgttcca
     1381 ggctctggag aagtctttcc cattcttggg tctttccgat gtgccggtgc tggcgatccc
     1441 tcaacgattc atttgtgaaa tcgggcaagg ccctgagttc cttctgtgat tgtttcacct
     1501 tttcatcagc ctgtgcgagg tattggcata cacccttgga ggcgggaggc tgtcctcccg
     1561 ctcgttcaaa gagtgcgaag gcacgatagc cctgttcttg gcctctaaat agggtttctg
     1621 ctgcttgata caaagcgcga gtctgttggt gctcttcctt ttgagcgaga atttttgcca
     1681 tgagagcggc cttcccaatg gagagtgcgg cgccctcagg gtctccattc gcaatcgcgt
     1741 cttcggcatg ttgctccagg cggtccattt ccccgagctc cccgatcatt tgtgtggagc
     1801 ctctttccgc atagccaacc ataagtacca taaagaaaca accaatgtag gtaagctggg
     1861 tggggatcaa aatcatgacg tcagctgatg gccgtcaaga tgaatctggc tgatgggacg
     1921 ttggctgaga ttcaagatag ctctgtctac tgaacctgcc aatccatatc gatttgttta
     1981 taggtccagc tcgtggcctc ccgcagttcg gccatttcct tcgaattgtc aaaaatcacc
     2041 cagattttgc tttgtagctc attaatcggg gcgatagact tttcatttcg tagtttgcct
     2101 aaactccaca cgacctctgt cagaaccggg aaatcgatgt ccgaagatga tttgatgtgg
     2161 ggtagatttt tcaccacata accaagcatt ggcggggtga ctgaaggatc tccggtatgg
     2221 gcgccaattt cccctaatcc tttggctgca gccgctcgaa tcttcgagtc tttctgtgtt
     2281 tgaaaaatat ccgttaagag gggaatgcct tcgctccccg tgctggaaag cgcgacgatg
     2341 gcctcttctg ccagttcagg atcttgaatg agttctttca gtagcgggag ggtatcgtgt
     2401 gattccactt gaggcagcag ggataaaatt cttagcttgc gtttattggg cgtctggggg
     2461 ttattgagta gcttgaacag gcgagcatca tgcccccatt ccgcagcgag cagaacggga
     2521 aaatcaaatt cttgaatccg ttcggccaat tgttgaattg cataaagacc ggattgtgga
     2581 gcgtgagcga ccttagcggc ttcacccgga tcttcaaaac tggttcgcgc ttctttgtac
     2641 caccctaagt ctcgtccatc caattgatag gaaaagaggc aggccgggcc tttccagagg
     2701 cgggatggag cgccggaatg gtccgcatca ccgccgccgc gagtggttcc cgaccatttt
     2761 tttacttctt cgcatttgac gttcaccctt acatcgaatg gagcatcgcg atcggttacc
     2821 acggtatagc ccaattcttc taaacgttgt ctcaccactt tttcgatgac tgttccatcc
     2881 actttgcccc gctctgtcaa cgccaagacg tgaatcataa tcgtttgaat gccttcgagt
     2941 tgcgcctttt gttcttggtc gagataggtc cgtcgggcaa aaccatctga accgaaaaac
     3001 ccaacgagca atactatgga gatgaatcca catgccgccc atgtcctcat gcctgatctc
     3061 ccacggtaca atgttgtgag gaattaactg agatggccat ccaagtctat ttgtttatag
     3121 gtccagctcg tggcctcccg taattcggcc atttcttccg aattgtcaaa aattacccaa
     3181 atcttctgct gaagctctct catgggttta agtgaaccct cccatcgttg cttgccaatt
     3241 gcccagacca cttcagtgag caccggaaaa tcaatatctt ccgaagtttt caattgcggt
     3301 aatagcgcag acacatactg cactaatggg ggaatcggac gggggtcgcc cgtcttggcc
     3361 gcaatatccc ccaaggcttt tgccgcttct gctcgaatac tggactgccg gctagtttgg
     3421 aataaatcaa tgagcaaggg aatggaatgc cctccagcgc ctgaaagcgc attgatggtt
     3481 tcctgctgca agtcggtgga ctcgagaagt ttggtcagtt gcggtagtgc ttcctcggca
     3541 tgcacatcgc tgaggacgga aagaattttc actttccgca gtttgggcgt attggggtta
     3601 tccagcagct tgaggaggcg atctatttga ccccattcgg ccgcgagcaa aaccgggaag
     3661 tcatattccg aaagccgttg gttgagctgg tccatcgcat aggtcccggc atcgtcaatc
     3721 tgggcctgtt tggcagcctg tctggcatcg gcaaaagtcg tgcgtacctc ttttttccat
     3781 tgcaaatcat tatgttggag gaaatacgtc aagagacagg ccggcccttt ccagagtcgg
     3841 ttgggtgcat cagccagatc tacgtctcca ccggtgggag aggtgccggt caccgtcttc
     3901 tgttcttcac attttacttt tacttccaca tcgtgaggtt gagagcgatc cgtggtcact
     3961 gtatacccca tgtcttccaa gcgcgccttg atggtggcga ccagtggact ggggtcatag
     4021 gtcccttttt ccgttagggc gaggacctcg accagtaccg tctgggcatt ctctaattgc
     4081 gttttttgct ctggtgtgag gtgtattctg cgggcctcgg atgccatgac cccgacacac
     4141 aatattgcga aaatgaggaa aaccggccag cgtgtcatga gtaacctcct tgtattatcg
     4201 caaagtccca accctcatga gtctcatggg ggcatgactt ggttaaaagg aagttgaacg
     4261 gaatttctta aattgtaccg tatattagca atacgacggc aacatggcag catcttgtaa
     4321 ttctattgcc ttataggtcc aattggtggc ctctcgtaac tcagccattt ctttcgaatt
     4381 gtcatgaatc aaccacactt tatcctgtag ttctgcaatt ggcttcaaag agtgagccca
     4441 tcgtaatttt ccaatcgccc agaccacttc agtgagcacc ggaaaatcaa tatcttccga
     4501 agttttcaat tgaggtaaga cttcggaaac atattccacc aatgggggaa tcgtgcgggg
     4561 atctcctgtc ctggctgcaa tatcccccaa ggcttttgcc gcttccgccc gcatgttgga
     4621 ttgaggactg gtttggaata aatcgatgag gaccggtatg gaatcggctc ctgcgcctga
     4681 aagcgcattg atggtttctt gctgcaagtc ggtggactcg agaagtttgg tcagttgcgg
     4741 cagtgcttcc tcggcatgca catcgctgag gacggaaaga attttcactt tccgcagttt
     4801 gggtgtattg gggttatcca gcagcttgag gaggcgatct atttgacccc attcggccgc
     4861 gagcaaaacc gggaagtcat attccgcgag gcgcaggttg agctggtcca tcgcataggc
     4921 tccggcatcg tcaacctggg cctgctgggc ggcctgcctg gcatccggaa aggtcgtgcg
     4981 cacttctttt ttccattgaa aattgttctg tttgaggaaa tacgtcaaca gacaggccgg
     5041 ccccttccag aggcggttgg gtgcatcagc caggtccacg tccccgccgg aggaagagat
     5101 gcgggtggag gttttagctt cttcacattt aactttcact gccacatcgt gcggctgaga
     5161 gcggtccgtg gtgaccgtat accccatgtc ttccaagcgc gccttgacgg tggcgaccag
     5221 tggactgggg tcaaagctcc ctttttccgt taaggcaagg acctcgacca atacggtctg
     5281 ggcattctct aattgcgttt tttgctctgg cgtgagagga atttggcggg cttcggagac
     5341 catgccccca acacccaata ttacgaaaat gaagaaaaca ggccaggctt tcatcccttc
     5401 ctccttgctt ctaattttta atcattgtgg attacaacgt aagcgaaatg gagacggaac
     5461 aaacgttttt cagacgaatt tacagggttg tctaatatac tcttcacgtt aagggagggg
     5521 caactgaatg ccatccggaa agctttccac caggtagtct cggcaagcga ctgatggccc
     5581 gacctcatgc ggggaatcgt aattgaaaag gtttattgct caattaaatg tttttttaga
     5641 aacaggtcct gagggcgtcg aatcctggga aatgctgttt gagggatttt gttcatattc
     5701 cctggagtga agttgaaccc ccatcagtct ccggctatac tcggtgaatg ggacagtcac
     5761 caaagtttgt atttttaggg gcaggcaaca tggctgatgc gttggtgacc ggaattttga
     5821 aagcccatct tgcatctccc gccaatattt ctgttaccga tatttcccca atccggctag
     5881 agcattttca gaagacattt caggttcatg tgggatctga taacgctgat gaagtgaaat
     5941 caggcgatat cattgttctc tgtgtgaagc ctcaggttat ggatacagtg ctggcagaga
     6001 tcaaagagca ggtttctcag aagcacttat tgatttccgt cgcagcgggg tatcctttgg
     6061 cacgcattca acagcatgtc gggagaaaca tttcccttat tcgtgcgatg cccaataccc
     6121 ctgcggtcat ccaggaaggc gtaacggctt tggccggaag ttcggggatc tccgcggacc
     6181 accttcaact ggctcaatca atctttgaat cggttggcaa agtcgtcatg gtggaggaat
     6241 ccttaatgga tgccgtgacg ggtttgagcg gtagcggtcc tgcgtatatc tatctggtga
     6301 ttgaagcact cacagacggt ggggttctgg tcggtcttcc ccggaccgta gccagtgtcc
     6361 ttgcgacaca aacggtatta ggggcggccc ggatggtgct cgaaagtggc gaacatcctg
     6421 ctgttcttaa agatcgggtg acgtcaccgg gtggaacaac gattgcaggt cttcaacaac
     6481 tagaagcagg gaagttacgg gcgaccttaa tgaaagccgt caaagctgct acggctcggt
     6541 cttatgagct aggacaataa ggacgtgggc ggccctaacg tttaatgcat acattgcata
     6601 aggttttatt gaaaggagta cacggtgcag tattgggtca aagttacctt tgttgataat
     6661 aaagaattag aattcaagga cgctattcgt cataccatca gcgat
//
LOCUS       DATIAL010000216         5657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1328821, whole genome shotgun sequence.
ACCESSION   DATIAL010000216 DATIAL010000000
VERSION     DATIAL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328821"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /gene="kaiC"
                     /locus_tag="VLA60_06745"
     CDS             <1..1442
                     /gene="kaiC"
                     /locus_tag="VLA60_06745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HSF09094.1"
                     /translation="KKSSGNPVHSEFPKAPTGIQGLDDITGGGLPRGRTTLVCGSPGC
                     GKTLLGIEFLVRGILESGEPGVMLAFEETAEELTQNVRSLGFDLDDLIKDNKLILDTI
                     PIEATEFEENGEYDLEGLFVRLGDSIDTIGAKRVVLDTIEVLFGGLSNQGIIRSELRR
                     LFQWLKNRNMTSIVTGERGEGSLTRQGLEEYVSDCVILLDHRVNEQMSTRRLRVVKYR
                     GSMHGTNEFPFLIEKQGLSVLPITSLILEHKTSKDKISTGIPQLDEMFGGEGVYRGST
                     VLVTGGVGTGKSSLVSHFADAACRRGEPAIYFAFEESSPQIIRNMKSIGLSLDKWVKK
                     GLLHIQASRPTLFGLESHLVNFYNAVHTHHPKLVIMDPINDFLSIGSTREIKALFLRM
                     VDFLKSRQITLICTALTTAGESSQDTEVGVSSLADTWILLSNLEFDGEHQRQLYILKS
                     RGMAHSHQVRGYTMNHRGWHLKEASVGNR"
     gene            1581..1949
                     /locus_tag="VLA60_06750"
     CDS             1581..1949
                     /locus_tag="VLA60_06750"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HSF09095.1"
                     /translation="MEKASKTSSKSKRHAAKDSPYHLTLYVAGHSPKSRLAMSNLQQL
                     CDTHLSSKYTVEIVDLLREPKRARLDQILAIPTLVKKVPPPLKKIIGDLSNAEKVLVG
                     LDLSDESGEVIMSSSVDGSS"
     gene            1946..2287
                     /locus_tag="VLA60_06755"
     CDS             1946..2287
                     /locus_tag="VLA60_06755"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HSF09096.1"
                     /translation="MSDTPGFTRKRGSARKDSGIRLRLYVTGATRLSLRAMANIKSIC
                     REYLEEEHPDLQIVDLYRTPSAARANHIFAAPTLIKEFPLPSCRIIGDLSKKDRVLHS
                     LGLAPSPPLTS"
     gene            2304..4418
                     /locus_tag="VLA60_06760"
     CDS             2304..4418
                     /locus_tag="VLA60_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09097.1"
                     /translation="MASKHKPSLAKYVPPSQEEILQQRLKEAEDTLQAIREGAVDALI
                     VETGEGDQVFTLKSADYPYRLIIDNMIQGAVIFDDAGIIFYANATFAGMVNHSHQELF
                     GFSLDLFISQEDRDFFRTLSSSHSHSAGGTGTIRLVPKDRHDLAVFVGLVSIKIDNQY
                     LHCAILTDLTEQKKYDRALVEEKFSRMILEQTGEAVVVCDLSGRIIRHSRVTDRLLGK
                     PILHTHFQTVFQFTPPAIVDSFGSAEEWDEGRKMIEEVLGGKTFHGLEVGLQVENTNV
                     PLILSGRPLQDDEENTIGAIMTMVDITDRKRAEQQLQDLNDSLEQRIVERTRALLSYQ
                     QHLREMTSELIGTEQRERRRLANELHDYLAQLLVVCRMKIAQLSQGGWERELKTAIQD
                     IDKILTDSLNYTRTLIAELSPNILYELGLAPALVWLGGQFEQHDLHVHIQEEEKDLQL
                     PEDHAIFVFQAVRELLFNVKKHAGTNHATVSLKRTASHELHVKVEDAGQGFPLEDTSQ
                     NYNRPGKFGLFSIRDRIEALGGTINIESEVGRGTCISLLVPIGSQIPSEVSQIPRSRP
                     LTSWAPSDLKRIQRKGIRILLVDDHALVREGIRGLLDVHADFHVVGEAQDGLEAIEYT
                     RMFHPDIIVMDVNMPKMNGIDATRKILREWPEVQVIGLSVQEDEQVRELMMQAGAVMY
                     LTKNGIANELVEGIRHLFLCSS"
     gene            complement(4481..5395)
                     /locus_tag="VLA60_06765"
     CDS             complement(4481..5395)
                     /locus_tag="VLA60_06765"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HSF09098.1"
                     /translation="MDYQGILEEIHQEILHLLPCGTVATYIPQLASVSSQKFGMAVQT
                     VGGEVFQVGHALEPFSMQSISKVYTLTLGFPFEGDKIWTRVGLEPSGSTFNSLVQLEY
                     EHGIPRNPFINAGALVISDILITHLKNAKPAFLDYVRQRANNAAIQDDPQVARSERET
                     GFRNAAMANFLKSFSNLTNEVEEVLEFYYFHCSLSMSCVDLAKGFLFLANKGHCPWTN
                     QQVLTQSQTKRVNALMLTCGTYDAAGDFAFNVGLPGKSGVGGGIVGIIPNRLTVAVWS
                     PGLNEKGNSLAGQYALELFTTKTGVSIF"
BASE COUNT         1439 a         1343 c         1469 g         1406 t
ORIGIN      
        1 ggaaaaaatc atcaggtaac ccggttcatt cagaatttcc aaaagcgcca accggcattc
       61 aaggcctgga tgacatcacg ggcggcgggc ttccccgagg acgaaccacg ttagtctgcg
      121 gaagccctgg ctgtggaaaa acattattgg gcattgagtt ccttgtccgc ggaattttgg
      181 aaagcggaga gcccggtgtc atgctggcat tcgaggaaac ggccgaggaa ttgacccaaa
      241 acgtccggtc cctggggttt gatctggatg acttaattaa ggataacaaa ttaattttgg
      301 acacgattcc tattgaggcc accgaatttg aagagaatgg agaatacgat ctcgaaggat
      361 tatttgtgcg gctgggtgat tctatcgaca cgatcggtgc caaacgagtt gtgttggaca
      421 ccatcgaagt cctgttcggc gggttatcca atcaggggat catccggtcg gagctgcggc
      481 gtctgtttca atggctgaaa aacaggaaca tgacctcaat tgtcaccggc gaacgcggag
      541 aggggtcttt aacccgtcag ggactcgagg aatatgtatc cgattgcgtc atcctgctgg
      601 atcatcgagt gaacgaacag atgtccaccc gccggttgcg tgtggtcaaa taccggggaa
      661 gcatgcatgg caccaatgaa tttccttttc ttattgaaaa acaggggctg tccgtcttgc
      721 ccatcacttc cttaatactt gaacacaaaa cttcaaaaga taaaatctca accggcattc
      781 cacaattgga tgagatgttc gggggagagg gtgtgtatcg gggttcgacg gttctggtga
      841 cagggggggt cgggacgggg aaatccagcc tggtctcgca ttttgcggat gccgcctgtc
      901 gacggggaga accggccata tattttgcct ttgaagaatc cagtcctcag attattcgga
      961 atatgaaatc gatcggactt tcattggata aatgggtgaa aaaaggcttg ttgcacattc
     1021 aagcgtcccg tccaacgctc ttcggtttgg aatcccatct cgtgaatttc tacaatgcgg
     1081 tgcacactca tcacccgaag ttggtgatca tggatccgat taatgatttt ttatccattg
     1141 gttcaactcg cgagatcaag gcgttatttt tacggatggt ggattttctg aaatcccgcc
     1201 aaatcaccct gatttgcaca gcccttacga cggctgggga atcttctcag gatacggagg
     1261 tcggagtgtc ctctctggcc gacacctgga ttctcctgtc aaatctggaa tttgatggtg
     1321 aacaccaacg gcaactgtat attttgaaat cccggggtat ggcgcattcg catcaggttc
     1381 gtggatatac gatgaatcac cgtggctggc atttgaaaga ggcgtcggtg ggtaaccgtt
     1441 aggactctga acttgatcgg cccaaccaag agatttaagc atgaagtagg gggcggcact
     1501 caaagaaaga ttgatcaaag ttgggaaaga ccgttggctg gtgatactga tgatcggtga
     1561 tcgagaaagg cctcactcct atggaaaaag cctctaagac ctcatcgaaa tccaagcggc
     1621 atgccgcaaa ggattctcct taccacttaa cactttatgt ggcaggacat tctcctaaat
     1681 ctcgtcttgc catgagtaat ttacagcaat tgtgcgacac ccatctgtcc tcgaagtata
     1741 cggtggaaat tgtggatctt ctccgggagc ccaagagggc caggcttgac cagattttgg
     1801 ctattcccac gctcgtcaaa aaagtaccgc ctcccttaaa aaaaatcatt ggagatttat
     1861 cgaatgcaga aaaggtctta gtggggctcg atctgagcga tgagtcgggt gaggtgatta
     1921 tgtcaagctc agtggatgga tcatcatgag cgatacacca ggcttcaccc gcaagagagg
     1981 ttctgcccgg aaagactccg gaattcgcct tcggctgtat gtgaccgggg cgacccgcct
     2041 ctcgctccgt gccatggcca atattaaatc catttgccgg gaatacctcg aagaagagca
     2101 tcccgacttg caaattgttg acttgtatcg caccccgtct gccgcccgtg ccaaccatat
     2161 ctttgccgct ccgactctca tcaaagaatt tccccttccc agttgccgga tcatcggaga
     2221 tctttcaaaa aaggaccgtg tgctgcattc tctgggattg gctccgtctc ctcctctgac
     2281 ctcttaggag ttggaaaata tccatggctt ccaaacataa accgtcccta gccaaatacg
     2341 ttccgccaag tcaggaggaa atattacagc aacggttgaa agaagcggaa gacacgctcc
     2401 aggcaatccg ggaaggagcc gtggatgccc ttatcgtcga aacgggggaa ggggatcagg
     2461 tctttacact caaaagcgcc gactatccgt atcgactcat catcgataat atgatccagg
     2521 gtgcggtaat ttttgatgat gccgggatta ttttttatgc gaatgccact tttgccggaa
     2581 tggtcaatca ttcccatcag gagctgttcg gcttttctct cgacttattc atttctcagg
     2641 aagatcggga ttttttccgg actctgtctt cttcccattc ccactcagcc gggggaacgg
     2701 ggactattcg cttggtgccc aaagatcgac atgatcttgc cgtttttgtc ggacttgtct
     2761 ccattaagat tgataaccag tatttacatt gtgcgattct aaccgacctg acggaacaga
     2821 agaaatacga ccgggccctg gtggaagaga aattttccag aatgattctg gaacaaaccg
     2881 gggaagccgt ggtggtgtgt gatctctccg gtcgaattat tcgccacagc cgtgtaaccg
     2941 atagactgct cggcaaacct atactccata cccacttcca gacagtgttt cagtttactc
     3001 ctcccgcgat cgtggattct ttcgggagtg ccgaagaatg ggatgaggga aggaagatga
     3061 tcgaagaagt tctgggaggc aagactttcc acggactcga ggtgggattg caggtggaga
     3121 atacgaacgt gccactgatc ctgagtggca ggccgttaca agacgacgaa gagaacacga
     3181 tcggggccat tatgacgatg gtggatatca ccgataggaa acgggccgaa cagcaattac
     3241 aggatttaaa tgactctctt gagcaacgca ttgtcgaacg cacgcgtgcc ttattgagtt
     3301 atcaacaaca tcttcgggag atgacgtcgg agctgattgg gaccgaacag cgggaacggc
     3361 gacgactggc caatgaactt cacgattatc ttgcccaatt attggtcgtc tgtcggatga
     3421 agattgccca attgagccag ggaggctggg aacgggaatt gaagacggcg atacaggata
     3481 tcgataaaat cctgacggat tcactcaact acaccaggac tcttattgca gagttgagcc
     3541 ccaatattct ctatgaattg ggactagcac ccgctctcgt ctggttggga gggcaatttg
     3601 aacagcatga tcttcatgtg catatacagg aagaagaaaa ggatcttcaa cttccagaag
     3661 accatgcgat atttgtcttt caagcagtcc gtgaactcct atttaatgtc aagaaacatg
     3721 ccggcaccaa tcacgccacg gtttccctca aaaggaccgc ctcccacgaa cttcacgtga
     3781 aggtggagga tgcagggcaa ggatttcccc tggaagatac ctcacagaat tacaaccgac
     3841 caggaaaatt tggactcttt agtattcgtg atcggataga ggctttgggg gggactatca
     3901 acatcgagtc ggaggtcgga cggggaacat gcatttcgct tcttgtccct attggttctc
     3961 aaatcccatc agaggtttct cagatacccc ggtcaaggcc tttgacctcc tgggcaccat
     4021 cggacctaaa acgaattcag cggaaaggta ttcgaattct ccttgtggat gatcatgccc
     4081 tcgtgaggga aggaatccgc ggcctattag atgtccacgc agattttcat gtcgtcggag
     4141 aggcgcaaga cggcttggaa gctatcgaat atacacggat gtttcatccc gatattatcg
     4201 tcatggatgt gaatatgcca aagatgaacg gcattgacgc gacacgaaaa atcttacggg
     4261 aatggccgga agtgcaagtt ataggattgt ccgttcagga agatgagcaa gtccgggaat
     4321 taatgatgca agctggggcg gtcatgtatc tgacgaaaaa tggcattgcc aacgaacttg
     4381 tcgaaggcat tcgtcatctt tttctctgtt cctcctagcc tccaggacgg atgccaatca
     4441 tctgttttta tccgtctgta ccacccgccg tcatcatgaa ttagaaaata gacaccccgg
     4501 ttttggtcgt aaagagttcg agggcatatt gtcctgcgag tgaattgccc ttttcgttta
     4561 agccggggga ccagacggca acggttaacc ggtttgggat gatcccgaca atcccaccac
     4621 ccacgccact tttccccggc aatccgacgt tgaaggcgaa gtcccccgca gcatcatagg
     4681 ttccgcaggt gagcatgagg gcgttcaccc tctttgtctg actttgggtg aggacctgct
     4741 gattggtcca aggacagtga cctttgttgg cgagaaagag aaatcccttc gccaggtcca
     4801 cacagctcat cgacagtgag cagtgaaagt aataaaactc caacacctct tccacctcgt
     4861 tcgtgaggtt ggaaaaactt ttgaggaaat tggccatggc cgcatttcga aagccggttt
     4921 ctcgctcgga gcgggccacc tgaggatcat cttgaatggc ggcattattg gcccgttgcc
     4981 tgacataatc gagaaaagcc ggcttggcat tttttaggtg ggtgatgaga atatcggaga
     5041 tcaccagggc tcccgcgttg ataaacggat ttctgggtat gccgtgctca tactcgagtt
     5101 ggacgagaga gttgaaggtc gatccggatg gttctagtcc gactcgtgtc caaattttgt
     5161 ctccttcaaa cggaaatccg agggtcagtg tgtagacctt tgagatactt tgcatcgaga
     5221 agggctctaa tgcgtgcccc acctggaaca cttcgccacc aacggtctgg accgccattc
     5281 caaatttttg agaggaaacg gacgccagtt gaggaatgta ggtcgcgaca gttccgcacg
     5341 gtaagaggtg cagaatttct tgatggattt cttctaaaat tccttgatag tccataagtc
     5401 ccaacgattt tcctaaaata tgagagtggt gaagtgaaag gatcagggtt catgggcata
     5461 tttttgtgga tgcttgctcc tgtcactcta gtctataact cttgcgcaaa gagaaatcta
     5521 cgctactcag gagtccgtgt tttagccgac actgtcctca acctattcgt catgcccgcg
     5581 gagggagtcg caaccgactt actgatcagg tccctaaatt gttcgtctca atatgagacg
     5641 ccgacattca gagacta
//
LOCUS       DATIAL010000217         8236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1220993, whole genome shotgun sequence.
ACCESSION   DATIAL010000217 DATIAL010000000
VERSION     DATIAL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220993"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VLA60_06770"
     CDS             complement(<1..315)
                     /locus_tag="VLA60_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09099.1"
                     /translation="MISLPFSANGQTYHLAIPIVGSLALGIFVIDYLTPLGIGIGSLY
                     ILVVVSTLTLESSLGTKIATIISIFLVFLGYWASSPPEPGYETAMLNRWISGFCILIT
                     GGI"
     gene            complement(353..961)
                     /locus_tag="VLA60_06775"
     CDS             complement(353..961)
                     /locus_tag="VLA60_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09100.1"
                     /translation="MPASPFETVKGLMPHGMCFSWDPFLLGLHVISDSLIIMSYFSIP
                     LALLYFTKKGGTFPFKYLIGLFSAFIFACGMTHVLSVWTIWRPDYYLEGAVKLLTAGI
                     SLVTAFMVWPVVLKVLSLPNLFILQDTNIQLEREIHKRQIIEHELRVQTNGLQEANEN
                     LLRLNRQMCGRELRMRELKKEINTLAQSIGRPTPYDLHEEQI"
     gene            complement(1552..1965)
                     /locus_tag="VLA60_06780"
     CDS             complement(1552..1965)
                     /locus_tag="VLA60_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09101.1"
                     /translation="MKTVLQEDLTGCGIACVATLANVSYQQVKSVAMTLGIDVGDTRL
                     WSHTAFLRILLNHYGIQSLPGEPPFLSWQALPPLALLATKWHRKNNQAFWHWVIYWKR
                     PDGPVVLDPKFTLRNHVRTDFGRIKPRWSITVQFM"
     gene            complement(2004..2999)
                     /locus_tag="VLA60_06785"
     CDS             complement(2004..2999)
                     /locus_tag="VLA60_06785"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HSF09102.1"
                     /translation="MGRTPHLQTQEPIRCGWVGTKPHFISYHDEEWGIPVHTDHRHFE
                     MLTLEGAQAGLSWSTILLRREGYRRAFAGFDPSKVAKFDNRKKEALLQDTGIIRNRLK
                     IESAISNAQAFLKVQEEFGAFDHYIWDFVGRAPKLNYWNTMSQVPASTSESDALSKDL
                     KKRGFKFVGSTVIYAHMQAAGLVNDHTTDCFRHPSNLSDQTTLQRTSNSRTASSSTIR
                     IKRVYDAPAPNDGCRILIDRLWPRGLSKEAAHINLWQKDLAPSTKLRNWFRHDPVRWP
                     EFQTRYFTELDQNIKAVEDLLERARYSPVTILFGAKNEKYNNAVALKAYLSRHFG"
     gene            3226..3738
                     /locus_tag="VLA60_06790"
     CDS             3226..3738
                     /locus_tag="VLA60_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09103.1"
                     /translation="MHSLISQRSARSFRSTGWIPFVCLWGVGLGVLMSSAHVIAQAPN
                     VPWECSEYSGEAQTRCMNVLLELQQNHIAKLEAQLNAQERTVNNLKEKMDRQEAFASR
                     DARVNKQDPLYSLTPYASPYAYAPLRPLGIYLQPPWTVSRYYGFGPAYWGQPGLSFNF
                     RYGRGHRHRH"
     gene            complement(3985..4350)
                     /locus_tag="VLA60_06795"
     CDS             complement(3985..4350)
                     /locus_tag="VLA60_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09104.1"
                     /translation="MAKLIEQPTRIKPAGNKPKLIDEFIGRVNCGTDQISIARMQSPS
                     GWEEPGQTPEFDEYTLVLSGQLRVETKEGVIEVNAGQAVIAYRNEWVRYSTPGPEGAE
                     YIAVCTQAFSPETVHRDPS"
     gene            complement(5212..6213)
                     /locus_tag="VLA60_06800"
     CDS             complement(5212..6213)
                     /locus_tag="VLA60_06800"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSF09105.1"
                     /translation="MPNGSYTLTAIARDAAGNTKTSASITVTVSNTSIPPADTTAPTV
                     TLTAPSADTVSGTVTVSASASDNVGVTGVQFRLQGANLGAEDTTNPYSTSWNTTTVPN
                     GSYTLTAIARDAAGNTKTSASITVTVSNTSTPPSPQNHDLNGDGKADLVWRNTKTGHV
                     AGWLLNGKSIADSGILGQLTMEWSLKGVGDLNADGHADIIWRNKTSGAVAVWLMDGLN
                     IASTGFPGTASTTWDIHGVGDLNGDRKADLIWRHTNGSTAVWLMNGTAPPSSTGSLGG
                     VAVPWQIVQVDDVDADGQADIIWRNEASGQVAVWLMDGLTRISVGFYGSASSDWEIR"
     gene            complement(6126..7514)
                     /locus_tag="VLA60_06805"
     CDS             complement(6126..7514)
                     /locus_tag="VLA60_06805"
                     /inference="COORDINATES: protein motif:HMM:NF044746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HSF09106.1"
                     /translation="MIRPTTTFGFVIARTVHLTLLLFGLFVSSAYSASSVQLTWNANT
                     ESDLAGYKIYKRALPSQDFGQPIFSGFPNNPSSPSTTVSGLSGGTTYGFIATAFDTAG
                     NESTPSTEATITITTSPPPADTTAPTVTLSAPSAGTVSGTVTVSASASDNVGVTSVQF
                     RLQGANLGAEDTTNPYSTSWNTTTVPNGSYTLTAIARDAAGNTKTSAAVTVTVSNTSP
                     PPPSGSSIFVNFQPSSASVPSGYQKDDGSVYTSSRGYGWTTFVNTRERNAQADQRLDT
                     FIHFDSGTSVTWNYDLPNGDYNVSLASGDPSWDQGPHHITIEGITVINNQTTATNQFI
                     TITNQPISVADGQLSLQLTRTSSTHTILNYVIITPASSDTTPPAVTLTAPNAGTVSGT
                     VTVSASASDNVGVTSVQFRLQGANLGAEDTTIPTAPAGIPPPCRMAPTPSRPSPVMRR
                     AIPKPRHLSRSP"
     regulatory      complement(7582..7663)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(7937..>8236)
                     /locus_tag="VLA60_06810"
     CDS             complement(7937..>8236)
                     /locus_tag="VLA60_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HSF09107.1"
                     /translation="DRGAQYASKAFRRLLKAHEFQGSMSRRRNCWDNAVVESFFGSLK
                     QERVQWCSYQTRYEAQQDVLNYIAMFYNRCRLHSYLGYISPNDFEKQLVNMKKVA"
BASE COUNT         1925 a         1936 c         2205 g         2170 t
ORIGIN      
        1 gatgcctccg gttatcagaa tacaaaagcc ggatatccag cgattcagca tggcggtttc
       61 ataaccgggc tcgggaggag aagacgccca ataccccaaa aacaccaaga atatggaaat
      121 gatcgtcgct attttggtgc ccaatgaact ttcgagggta agcgtagaca ccacgaccag
      181 aatatagaga ctgcctatcc cgattcccag tggagtcagg taatcgatta cgaagattcc
      241 tagtgccaga gacccgacga tgggaatcgc gagatggtat gtctgaccgt tggccgagaa
      301 gggtaaagat atcatggttt tcctgattca gatcgcatga gatccctatt tttcagatct
      361 gttcctcatg gagatcatac ggggtggggc gtccgataga ttgggctaag gtattgatct
      421 cctttttgag ttcgcgcatt cgcaattccc ttccgcacat ttgtcgattt aatcgaagga
      481 gattctcgtt agcttcctgg aggccattgg tttgcacgcg caattcatgt tcaatgattt
      541 ggcgtttgtg aatttcccgt tccaattgaa tgttggtatc ctgcaagata aacagattgg
      601 gtaacgacaa taccttcaat accactggcc ataccatgaa ggcggtgaca agggaaattc
      661 ccgcagtgag aagtttcacg gccccttcca ggtaataatc cggcctccag atggtccaca
      721 cgctcagaac gtgtgtcatg ccgcaagcaa aaataaacgc actaaacagt ccgatcaggt
      781 atttgaaggg aaatgtcccc ccttttttgg tgaaatacag gagtgccagt ggaatggaaa
      841 aataggacat gatgatgagc gaatcggaga tgacatgcaa cccgagcaaa aagggatccc
      901 acgaaaaaca catcccatgg ggcatgaggc ctttcacggt ttcgaagggg gatgccggca
      961 tagtgtgctc cttcattcaa ggaagtcgtg ttttaacctt atctcctttc tctcgcgtgg
     1021 gtctcgtgag accgttcttc cctgaggata tctggaaacc caggattgat acgcgtggga
     1081 gcaggaataa tgaggatcgc aaaagtagac acttgtcacc gtgtacgttt tgacgatggg
     1141 agatgaaaaa atttgctctg gataaaacgg atgaaattta aaactggttc attgttgtga
     1201 gaaatgccga attctaagtt acattcgaat gcttctgatg gttgtgcacc gaacaaacat
     1261 ttgatgaaac aaacatgaag catctctcgg cataatgtat attaggcagg atgtatgtga
     1321 tgaagatgag atataaaaat aagttcgata ttaccgtttt tatcaatgaa catcaaggga
     1381 taatacctac ctgaaataga gcaaaggttc attccttttt aatcaattca aaaagatggc
     1441 agatcgaatt tgatgtcatt cgggaaggcc ttcatccggt tgattggtaa ttgaaacgta
     1501 tttcccatgt aggggattct gtcatgggaa ccatcctttc ttcgaggcga cctacatgaa
     1561 ttgaacggta atcgaccatc ggggtttgat tcttccgaaa tcggtacgga cgtgattgcg
     1621 gagggtgaat ttaggatcca acaccactgg tccgtctggc ctcttccaat aaatcaccca
     1681 atgccaaaag gcctgattat ttttgcggtg ccacttcgtg gcgagaaggg cgagtggcgg
     1741 cagagcctgc caggatagaa aggggggttc tcccggcagg gattgaattc cataatggtt
     1801 taacaatatc cgaaggaagg ccgtatggga ccagagacgt gtatccccga cgtcaatgcc
     1861 caatgtcatt gccacggatt tgacttgttg gtaagagacg ttggctaaag tagccacaca
     1921 ggcgattccg catcctgtga gatcctcctg aagaacggtt ttcatgtggg ttggtccaac
     1981 agccatggaa ggataccgta ccctcatcca aaatgacggg agagataggc tttcagggcc
     2041 accgcattat tgtatttctc attcttggcc ccaaaaagaa tcgtcaccgg actatatcgg
     2101 gctcgttcca gaagatcctc aacggccttg atattctgat caagctcggt gaaataccgt
     2161 gtctgaaatt ctggccaacg gaccgggtca tgccggaacc agttacgcag ttttgttgac
     2221 ggggcaagat ctttttgcca caaattgatg tgagccgcct cttttgaaag tccacgtggc
     2281 cacagccgat caatgagaat ccgacatccg tcattggggg caggagcgtc gtacactcgt
     2341 ttgatcctga tggtcgagga tgatgctgtt cgggaattgc tcgttctttg taaagtggtc
     2401 tgatcagaaa ggtttgaggg atgtcgaaag caatccgtgg tatggtcatt gaccaatccg
     2461 gccgcttgca tgtgtgcgta gataacggta ctccccacaa atttaaaccc tcgcttctta
     2521 agatcttttg aaagagcatc gctttcagag gttgaggccg ggacttgaga catggtgttc
     2581 caataattca acttgggtgc cctcccaaca aaatcccata tatagtgatc aaaggccccg
     2641 aactcctcct gcaccttaag gaaggcttgg gcattggaaa tggcggattc gattttcagg
     2701 cgatttcgga tgatcccggt atcttgaagg agtgcctctt tttttctgtt gtcaaatttg
     2761 gccactttcg agggatcgaa tccggcaaac gcccggcggt agccttcgcg gcgtaacagg
     2821 atcgtcgacc aggacaaccc cgcctgcgca ccctccaatg tcagcatttc aaagtggcgg
     2881 tgatctgtgt ggactgggat tccccattct tcatcatgat aggaaatgaa gtggggcttt
     2941 gttcccaccc atccacaacg gataggttct tgagtctgta ggtgtggggt gcgtcccatg
     3001 gaaaatttct tgctctctcg gatgactcgt gcgcctgaat cttctctatt cgctataagt
     3061 ggaaatgaat gtgagtattc ggtgtgtgga aaaggctacg aatttctgcc caacctgtca
     3121 acaaatatta agaatcacat ggattgggac tgggaggtgg ttcatttgtc ggattttgaa
     3181 atgccatgat acagtatatt ggtaccttca cagaggaggg gaatcatgca ttcattgatt
     3241 tcacaacgat cagcaaggtc ttttcgttca acgggatgga tcccatttgt ctgcctgtgg
     3301 ggagtcgggt tgggggtgtt aatgagtagt gctcacgtca tcgctcaagc acccaatgtt
     3361 ccctgggaat gctccgaata ctccggtgag gctcaaaccc gatgtatgaa tgtgttgctg
     3421 gagcttcaac aaaatcacat tgccaagttg gaagcacaac tcaatgccca ggaaaggact
     3481 gttaacaacc tcaaagagaa gatggaccgt caggaagcgt tcgcctctcg tgacgctcgg
     3541 gttaataaac aagatcccct atattcactg accccctatg cttctcccta tgcctatgcg
     3601 ccgttgcggc cactcggtat ctatcttcaa cctccctgga ccgtttcacg atattatggg
     3661 tttggtccgg catattgggg acagccaggc ctgtcattta attttcgata tgggagaggc
     3721 cataggcata gacattaaga tgtctcggca tttcctttat gtgtctgggg cttgcgttgt
     3781 ttcagagtgg gtgatcttcg ctcaatcttt tttattcacc taaatgtccc ttctgcacat
     3841 tgcgagtggt ggcatttccc aatctacatc atggggtaac gcgttccatt ttgctcgtat
     3901 aggattggct tgggcgttat ccactccatc tgtcttctgc ttcggtgact ttaagaaacc
     3961 gatcagatgt gaatcggcaa agattcacga gggatcgcga tgcacggtct ccggggaaaa
     4021 ggcctgggta cacaccgcga tatactcagc tccttcaggg cccggggtgc tgtatcgcac
     4081 ccattcatta cgataggcga tgaccgcctg accagcattc acctcaatca ccccttcttt
     4141 cgtttccaca cgtaattgac cggaaaggac caacgtatat tcatcgaact ccggggtctg
     4201 gccgggctct tcccatccgg aaggactttg catccgcgca atgctgatct ggtctgtgcc
     4261 gcaattgacc cgtccgataa attcatcgat gagtttgggt ttattccctg caggtttaat
     4321 tcttgtgggt tgctcgatca gtttagccat actgtctcct tgacctcctt ttcgtgtatt
     4381 gaagtgcgca tcgccagggg gcttcccctg gtcaaatttt atcctaggcc cactggcctg
     4441 tcatcgtgct catgtttgtt gcacgcttgt ttaattaatc atcgtaattg ccattgaagt
     4501 ttccatatgg cgaccgaaac tcctctgtcg ggctggaaca atagaatgat gggagccgca
     4561 gggttgagga aattaacgta aagccgttcc atctcactat cccatagggg aaacggtaaa
     4621 ccacaataga atgtttatgg caatctttca agaaaattta tttatagtca tttttaattg
     4681 ataacaaata gtccgaccag ttccaaattt agaatagcct cagatacctt ctggcagcaa
     4741 ggaagaggtg gattcaagtt taaagtgcca cgacctctgt aggatgatct gtggctttgg
     4801 agaaatgata cggtaccatg agtaagatcc tagccttgtc tgtcaaaatt catctacacg
     4861 gtatttattt cacgaacaaa ttgtaatctg attgtgccaa aaccttccga gggattctgg
     4921 gagccacctc ccatcaaact attcatgtca gtacttggcg ggtttgccca actggcgggg
     4981 aggagtttca ggattgatgg cgctaaggca aatgccgaca tgggggtcgg atgtgttacc
     5041 tgcatattct tggaagctgt aataggacca acgggtgtgg tggatatccg tgtgccgcgt
     5101 agttgcgcgg gaagaatgat tattcctggc aggggcttgt tactaacaga gagtcgggat
     5161 ggcccatgag gccatcccag ttctgtattc ttgtgttttt aacaatcgtg cctaccgaat
     5221 ttcccagtct gaggaagcgg aaccgtaaaa gcctacggaa atcctggtca agccgtccat
     5281 cagccatact gctacctggc cgctcgcttc attgcgccag atgatatccg cctgtccgtc
     5341 cgcatccaca tcatccactt gcacaatctg ccacggtacg gctactccgc ccagtgagcc
     5401 ggtggaggag ggaggcgccg ttccattcat taaccatacc gcggtgctgc cattcgtgtg
     5461 ccgccaaata aggtctgctt ttcggtcacc gtttagatcc ccgactccgt gaatatccca
     5521 tgtcgttgac gctgtgccgg gaaagcctgt ggaggctatg ttcaggccgt ccatcagcca
     5581 taccgctacc gcgccgctcg ttttattgcg ccagatgata tccgcgtggc catccgcatt
     5641 cagatccccc actcccttca gggaccattc catagtcaac tggcccagaa tgcccgagtc
     5701 cgcaatgctt ttgccattga gcagccatcc agccacgtgc ccggtttttg tattccgcca
     5761 gaccaggtcg gccttaccgt ccccattcag atcgtgattc tggggtgagg gtggcgtgct
     5821 ggtattgctc acggtgaccg tgatagatgc cgaggttttg gtattgcccg ccgcatcacg
     5881 ggcgatggcc gtgagggtgt aggagccatt cggcacggtg gtggtattcc agctggtgct
     5941 gtagggattg gtcgtatctt cggcgccgag gttggcgccc tgcagccgga attggacacc
     6001 ggtgaccccg acattatcgc tggcgctggc actgaccgtc accgtgccgg agacggtgtc
     6061 cgcactgggg gccgtcagcg tgaccgttgg ggcggtcgta tctgcgggcg gaatgctggt
     6121 attgctcacg gtgaccgtga tagatgccga ggttttggta ttgcccgccg catcacgggc
     6181 gatggccgtg agggtgtagg agccattcgg cacggtggtg gtattccagc tggtgctgta
     6241 gggattgtcg tatcttcggc gccgaggttg gcgccctgca gccggaattg gacactggtg
     6301 accccgacat tgtcgctggc gctggcactg accgtcaccg tgccggagac ggtgcccgcg
     6361 ttgggggccg tcagtgtgac ggccggaggt gtggtatcgg atgacgccgg ggtgataatc
     6421 acataattca gtatggtatg tgtagaagat gttcgagtca gttgcagact caattgccca
     6481 tccgcgacgc taatgggttg gttagtaatg gtaatgaatt gattggtagc ggtcgtctga
     6541 ttattaatga cggtgatgcc ttctatcgta atatgatgcg ggccttggtc ccaactgggg
     6601 tcgccgctgg ccagtgaaac gttatagtcg ccatttggta gatcgtaatt ccaggtcacg
     6661 ctggtgccgg agtcaaaatg aatgaatgtg tcgagccgtt gatcggcctg ggcattccgc
     6721 tcgcgggtgt tgacgaaggt ggtccagccg tagccacggc tgctggtata tacactgccg
     6781 tcatcttttt gatagccact tgggacggat gcgctggagg gttggaaatt gacgaagatg
     6841 gacgaaccgg aaggtggtgg tgggctggta ttgctcacgg tgacggtgac ggccgcggag
     6901 gttttcgtgt tgcccgcggc atcgcgggcg atggccgtga gggtgtagga gccattcggc
     6961 acggtggtgg tattccagct ggtgctgtag ggattggtcg tgtcttcggc gccgaggttg
     7021 gcgccctgca gccggaattg gacactggtg accccgacat tgtcgctggc gctggcactg
     7081 accgtcaccg tgccggagac ggtacccgcg ctgggggccg atagtgtgac tgtcggggca
     7141 gtcgtatctg cgggtggggg gctagtggtt atcgtaattg ttgcttctgt agaaggggta
     7201 ctttcatttc ctgctgtatc aaatgctgtc gcaataaatc cataggtagt tcccccactg
     7261 agcccggaaa cggttgttga gggggaggat ggattattgg gaaatcctga aaatataggt
     7321 tgtccaaaat cttgagaagg taatgcccgt ttgtaaattt tatatcctgc caaatccgat
     7381 tcggtgtttg cattccaggt tagttggaca gaggaggccg aatacgcgga gctaacgaac
     7441 aatccgaaga ggagaagtgt gaggtgtacc gtacgcgcga ttacgaaacc aaaggtggtg
     7501 gttggtcgga tcatactctt ctccttgtcg tattcggacc gaagtccgca ttttctcctc
     7561 cccctttgga ttaggtcttc cttcggtagc cggacgatca ggcgttacta cctgactcct
     7621 ggcattgcgg ccctacttca cagtaggtgt gcccttttcg ggaagaggat tgtttgaact
     7681 aatgggtggt caatgcatgt atcccttcat ttttcatttt ttcgcacctc taaacggtct
     7741 gccgaacatt tcggagaagt aaggagaaac tttagagagg attaggatga cgaggcggtt
     7801 ggtcggaaaa tttataagag caaacatgag aaactataaa aatagaacaa aaaggccatg
     7861 gaaataatga aatacttgaa ctcaattttt tatttttcat ccatttgacc cggtcaagct
     7921 atttgggaca tcccagttaa gcaaccttct tcatgttcac caattgtttc tcaaagtcat
     7981 ttggactgat gtagcccaga tatgaatgca accggcatcg gttataaaac atggcgatgt
     8041 aattcaacac atcttgttgg gcttcataac gcgtctggta gctacaccac tgcacccgtt
     8101 cttgcttcag agaaccaaag aagctttcca ccaccgcatt atcccagcaa ttcctccgac
     8161 ggctcatgct gccttgaaac tcgtgggctt tgagcagtcg ccggaatgcc ttgctggcat
     8221 actgtgcccc ccgatc
//
LOCUS       DATIAL010000218        12703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_791158, whole genome shotgun sequence.
ACCESSION   DATIAL010000218 DATIAL010000000
VERSION     DATIAL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791158"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VLA60_06815"
     CDS             complement(<1..581)
                     /locus_tag="VLA60_06815"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSF09108.1"
                     /translation="MKKQLFETPILFIVGITFSIMFSGIYDHIIYAEEVPVKEFSITV
                     EEMTITLLEDPKKEVTVWAYALKGEKPSVPGPVIRVNKGDLVKVHFTNTHHLPHTMHF
                     HGVHPFNMDGNGQRTMGREQLQMPGESYTYEWVAEDPGYYLYHCHFDTANHMDHGMYG
                     LFVVEDPAWPQVDQEFVTFWDEWDTNDDGHYDT"
     gene            complement(611..1900)
                     /locus_tag="VLA60_06820"
     CDS             complement(611..1900)
                     /locus_tag="VLA60_06820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSF09109.1"
                     /translation="MTSKPLSFPSTISAGTSHKPRFLEAFAKRALLNRLNQITNGEII
                     LNDGSTYLRCGRVTAQCHLKVTVTVTNPRFYTDTVFGGSIGAGEAFMAGYWQVDDLPA
                     LIRIFLCNRELLDGMDGRWACLTMPIQKFLHRLNRNTTKGSRRNISAHYDVGNDFFAL
                     FLDESLMYSSAIFTDSTMTLHEAQVARLDHICRKLELKSTDHLLEIGTGWGGFALHAA
                     TRYGCRVTTTTISQQQYDLACDRVAAAGLDDRITVLCTDYRDLTGEYDKLASLEMIEA
                     VGYHYFDSYFGACSRLLKTEGMMLLQAITIADQRYEAAKRSVDFIQRYIFPGSCIPSI
                     TAMSGAIARASDLRLFHLEDIGPHYVTTLQAWRDNLYRNRQQILRLGYAEEFFRMWEF
                     YFCYCEGGFTERVISDVQMLLTKPGNRREPIPSIQVA"
     gene            complement(1897..2691)
                     /locus_tag="VLA60_06825"
     CDS             complement(1897..2691)
                     /locus_tag="VLA60_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HSF09110.1"
                     /translation="MQSCLYHGRIRHRRFEPKAHSFDYPVFYAYLDLDELDKVFEDRW
                     FWSSKGPAPVRFRRRDYLGDAEIPLQTAVRDHIRRETGHTPDGPIRLLTHLRHFGFSF
                     NPVSFYYCFDARSQFPETIVAEITNTPWNERHAYVLPRHSSLSQTEHLRFRFGKAFHV
                     SPFMPMNIQYDWTFGTPGTHLSVNMKNFHHNRRIFDATLILEREPINGISCARDLAGY
                     PLMPIKVFGAIYWQALKLFLKRVPIFTHPSKIVQDPQGKARSADSL"
     gene            complement(2645..3937)
                     /locus_tag="VLA60_06830"
     CDS             complement(2645..3937)
                     /locus_tag="VLA60_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF09111.1"
                     /translation="MKIAIIGTGIAGNVAAYHLSRDHRITVFEANDYVGGHSHTHDIE
                     WEGQGYQLDSGFMVFNHQTYPCFTRLLKELDVPAQASSMGFSVKCDRSGLEYNGTSLN
                     TLFAQRRNLLRPSFYRMISDILRFNREASQCLANPDMTATLGNYLKQAGYGREFLERY
                     LIPMGAAIWSADPSQMWAMPAQFFIRFFHNHGMLSVNDRPTWYVVRGGAQTYVNKLTA
                     SFRHRIRTNAPVESIKRNRDYVTVTGLHGSPERFDAVFIATHGDQALRLLSDATDLEG
                     EVLGPMTTQKNEAVLHTDAGMLPARRRAWAAWNYHIPVHTQNRVAVTYNLNILQGLRA
                     PVQFCVTLNNSRDISPTKILKRMIYAHPIFSVPSVRAQQRQAEINGRNRTYFCGAYWR
                     YGFHEDGVVSALNALEHFKRTTHHAELPLPRTNTASAV"
     gene            complement(4102..4638)
                     /locus_tag="VLA60_06835"
     CDS             complement(4102..4638)
                     /locus_tag="VLA60_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HSF09112.1"
                     /translation="MHTSSLIACGLLGSLLGCSSPPPIQIASQVNLERFMGDWYVIAN
                     IPTFIETEAFNAVESYRLNDDGTIATTFTFRKGGFEGERKVYRPTGFVVDQESNAVWD
                     MQFLWPFKADYRIVFVNPDYSQTIIGRLKRDYVWIMARPPLLPEEDYQHLLNIIAAQG
                     YDLSKIQRVPQRWGDGQN"
     gene            complement(4651..5322)
                     /locus_tag="VLA60_06840"
     CDS             complement(4651..5322)
                     /locus_tag="VLA60_06840"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HSF09113.1"
                     /translation="MIGTFSMIEGSSLLLTLMAFFIFSIGLAISAQTTNQKTYSFKVF
                     LEDDEIGLQRFVVSTKGTRTQINVEAEFDVKYFFITFYTYRHTNAEVWEGECLKEIRA
                     KTNDNGESFFVEGTSKGGTLRLHTHDEEQTLEGCIKTFAYWNPAWFQSSRLLNSQTGE
                     LQPVEIQTVAEEPFTVRGVTTPTHHHRIISDKFTIDLWYTMDDEWVGLQSTTKDGKKL
                     RYELQ"
     gene            complement(5319..6125)
                     /locus_tag="VLA60_06845"
     CDS             complement(5319..6125)
                     /locus_tag="VLA60_06845"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HSF09114.1"
                     /translation="MTFSIYLSGLHAILVLAALTWLVSLIKRDVSIVDSMWSVMIFAS
                     AFVYSSSVEPYWNRSSLVLTLVLLWALRLTVYITWRNWGESEDARYQAIRRKYEPNFA
                     LKSLGIIFVFQAVLAWIISMPLWVALTVPFEYSIFDALAVAVWVIGMFFESVGDWQLA
                     RFKMNPANRGKVMNQGLWRYTRHPNYFGECLIWWGFFLFVIPTGAWWSILSPILLTFL
                     LLKFSGVTMLEETIVDRRPAYREYMASTNIFIPGPQKETNSLADHQEEIS"
     gene            complement(6122..6661)
                     /locus_tag="VLA60_06850"
     CDS             complement(6122..6661)
                     /locus_tag="VLA60_06850"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 domain-containing protein"
                     /protein_id="HSF09115.1"
                     /translation="MARTLCNFLLFQIGWFACVLSGAGQRPWIGALVALVIVAFHLFS
                     APAAEAEFKLVMIALVIGAVWDSMLVWLDWLHYSSGILIPHTAPYWIVLMWGLFATLL
                     NVSLRWLRGRWLIAALAGSVGGPLAYYGGHQLGALEFGNQNAALAALAVGWAVITPIL
                     MALSTRFDGFAPRLKESTI"
     gene            complement(6673..7725)
                     /locus_tag="VLA60_06855"
     CDS             complement(6673..7725)
                     /locus_tag="VLA60_06855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HSF09116.1"
                     /translation="MSILFTLARSLTDRGVIPDAVLRYSIQHLVRQRLKEISYDEQAL
                     PSYKLSFIKDIGRSSIAPFPEKANSQHYEVPAEFFQKVLGPQLKYSSAFWPEGIESLP
                     EAEEVSLRETKVHADLHDGQSVLELGCGWGSLTLCIAAQYPHSHITAVSNSHSQKAYI
                     DSQVQKRGLRNIRVLTCDMNKFHIEHSQFDRVVSVEMFEHMRNWENLFDRIQCWLKPD
                     GRFFMHIFVHRAVPYKFEVRDSSDWMSEHFFSGGMMPSDDLPLAIQSPFKLVNRWRWD
                     GTHYERTANAWLENMDHHREALWPLFIQTYGKPNAGLWWIRWRIFFMACAELFGYREG
                     QEWWVSHYLFEKADLT"
     gene            complement(8230..9660)
                     /locus_tag="VLA60_06860"
     CDS             complement(8230..9660)
                     /locus_tag="VLA60_06860"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HSF09117.1"
                     /translation="MPTNESLAIVWFRQDLRIKDNPALLAAHQTGHPIVPLFILDEEN
                     SGQWKLGAASRWWLHESLKALNEALEGKLCFKAGSAEKVLIDVMKEVGAEAIYWNRCY
                     EPWRITRDGHIQETLQTKGIAVHTFNGSLLFEPQTALKSDGTPYKVFTPFYQRGCLGK
                     GSEPRQPQKALKDLRLFTHRGVALKDLDLLPKIPWHTKLVKHWEPGEAGAQARLKAFL
                     KGGLKEYKEGRNYPSRNNVSRLSPHLHFGEISPNEVWHAAKHRMSTEGWRKDGETFLS
                     ELGWREFSHSLLFHFPELPRKNLQPKFNSFPWRKDPKALKRWQQGQTGYPIVDAGMRE
                     LWETGYMHNRVRMIVGSFLVKNLMLHWHLGEDWFWDTLVDADLANNSASWQWIAGCGA
                     DAAPFFRIFNPVTQGKKFDENGEYVRRFIPELKKMPVKYLHNPWEAPEKVLAEAGVQL
                     GEDYPLPIVTLSDSRERALEAFSELA"
     gene            complement(9837..10997)
                     /locus_tag="VLA60_06865"
                     /pseudo
     CDS             complement(9837..10997)
                     /locus_tag="VLA60_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
     gene            11614..12060
                     /locus_tag="VLA60_06870"
     CDS             11614..12060
                     /locus_tag="VLA60_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSF09118.1"
                     /translation="MIGLVGWLGGCDLPEPPKAAEAIPSVYANLHMPDGWWTDPTIIE
                     DGRQLYLGLNKSNVNCAQCHGKTGKPVMTAALSFLDVDKMKRYSDSQMLWRISEGIPY
                     TRMGAHKDKLSQDEIWKIIAFVSTLGLDGWQYDPNSKSWVPNEVRP"
     gene            complement(12383..>12703)
                     /locus_tag="VLA60_06875"
     CDS             complement(12383..>12703)
                     /locus_tag="VLA60_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HSF09119.1"
                     /translation="YFPPTDVRIAYLMLSVRETVCEWKGMARYWSIGIGKRMAQNAAW
                     SYPHPGKGYEYISNYLAFYPGKVDGCYVGKQKVKPQSGEFYGGWITSKILGPFKGEPG
                     TELW"
BASE COUNT         3100 a         3141 c         3228 g         3234 t
ORIGIN      
        1 tgggtatcgt agtggccatc atcatttgtg tcccactcgt cccaaaatgt gacaaattcc
       61 tgatcgactt gaggccaagc aggatcttcg acgacaaata atccgtacat gccatgatcc
      121 atatggttcg cggtgtcaaa atggcaatga tataaataat agccaggatc ttccgccacc
      181 cattcatagg tgtaggattc accgggcatc tgaagttgct cccgtcccat ggttcgctga
      241 ccatttccat ccatgttaaa cggatgcaca ccatggaaat gcatggtgtg aggaaggtga
      301 tgcgtgttgg tgaaatggac cttgaccagg tcgcctttgt taacacgaat gaccgggccg
      361 ggaactgaag gcttttctcc ttttagggca taagcccaaa ctgtcacttc tttcttggga
      421 tcctccagta acgtaatagt catttcctcc accgtgatgg agaattcttt gacgggcact
      481 tcttcagcgt aaatgatgtg atcataaatt cccgaaaaca tgattgaaaa agttataccc
      541 acgataaaca gtataggcgt ttcaaacaat tgttttttca cggtgtcctc cattggactc
      601 ggtggatctt tcacgctacc tgaatggaag gaatcggttc tcgacgattt ccaggtttgg
      661 tcagtagcat ttgaacgtca ctgatgacgc gttccgtaaa tcctccctcg caataacaaa
      721 aatagaattc ccacatacgg aaaaattctt cggcatagcc cagcctcaga atctgttgcc
      781 ggttccgata gagattgtcg cgccaggctt gcagcgtcgt gacataatga ggtccaatat
      841 cctcaagatg aaatagtcgc agatcactgg ctcgtgcgat agccccgctc atggcggtga
      901 tggagggaat acagcttccc ggaaaaatgt accgctggat gaaatccacc gatcgtttgg
      961 cggcttcgta tcgctgatcg gcgatcgtga tggcctgcaa caacatcatc ccttcagttt
     1021 tcaggagccg gctacatgct ccaaaatagg aatcgaaata atggtatcct accgcctcga
     1081 tcatttctaa ggaagccagt ttgtcgtatt cccctgtgag gtcccgataa tccgtacaca
     1141 gcaccgtaat gcggtcatcc aggcctgctg cagccacccg gtcacaggcg agatcgtatt
     1201 gctgttgcga tatggtcgtg gtcgtcaccc gacagccata gcgggtggct gcatgcaggg
     1261 caaaaccgcc ccatccggtt ccgatctcga gaagatggtc tgtggatttc agttccagct
     1321 tgcgacagat gtggtccagc cgcgctacct gagcctcatg aagcgtcatc gtcgaatcgg
     1381 tgaatatggc gctggagtac atcaggcttt catccagaaa tagggcaaag aagtcattgc
     1441 ccacatcata gtgggctgaa atgttccgtc ggctcccttt tgtcgtgttc cggttcagtc
     1501 ggtggaggaa tttctggatg ggcatggtta agcaggccca ccggccatcc attccgtcca
     1561 gaagttcccg attgcataag aaaatgcgta tcagggccgg gagatcatca acctgccaat
     1621 agcctgccat gaaggcctct ccggccccga tggatccacc aaacaccgta tcagtataga
     1681 atcggggatt ggtgactgtg accgtgactt tcagatggca ttgcgccgtg acacggccgc
     1741 accggagata agtgctccca tcatttagga taatttcccc gttggttatt tggttaagtc
     1801 ggttgaggag ggcgcgcttg gcgaaggcct ccagaaatct gggcttgtga gaagtgcccg
     1861 ccgaaattgt tgacgggaat gataggggtt tcgaagtcat aggctgtccg ccgacctggc
     1921 ttttccttgt gggtcctgca caatttttga ggggtgagta aaaattggta cgcgtttaag
     1981 aaagagtttc agtgcttgcc aataaatagc gccgaagacc ttgatgggca taaggggata
     2041 gccggccaag tcgcgagcgc agcttatccc gttgatcggt tcgcgctcca atatcagtgt
     2101 cgcgtcaaag attctccggt tgtggtggaa gtttttcata ttcaccgaaa ggtgtgtgcc
     2161 gggggttccg aatgtccaat cgtattgaat gttcatgggc ataaatggcg agacatgaaa
     2221 cgccttccca aaccggaagc gtaaatgttc ggtctgggaa agactggaat gtcttggaag
     2281 gacataggca tggcgttcgt tccatggggt gttggtgatc tccgctacaa tcgtctccgg
     2341 aaattgagac cgggcatcga agcagtaata aaaactaacc gggttgaacg aaaacccgaa
     2401 atgtcgcaga tgcgtgagca accggatagg tccatccggg gtgtgtcctg tttcccgccg
     2461 gatgtgatcc cggacagctg tttgcagagg gatttccgca tctcccagat agtcccttcg
     2521 tcgaaatctg accggtgcag ggcctttgct tgaccagaac caacggtctt cgaagacctt
     2581 gtcgagttca tcaagatcaa gatacgcata gaacaccggg taatcgaacg aatgggcttt
     2641 cggttcaaac cgccgatgcc gtattcgtcc gtggtagagg caactctgca tgatgcgtgg
     2701 tccttttgaa atgttccaat gcgttcaggg cactcacgac tccatcctca tggaagccgt
     2761 atctccagta ggcaccgcaa aaataggtcc ggttccggcc gttgatttcc gcctgtcgct
     2821 gttgcgcgcg gacactggga acagaaaaaa tcgggtgagc ataaatcatg cgcttcagga
     2881 ttttggtggg ggagatgtcc cggctgttat tcaatgtgac gcaaaattga acgggggcgc
     2941 gaaggccctg caaaatattc agattgtagg tcaccgcaac ccgattttgt gtgtgaaccg
     3001 gaatatggta gttccaggca gcccaggctc gtcgacgtgc gggaagcatt ccggcatcgg
     3061 tgtgcaggac cgcctcattc ttctgtgtgg tcatcggacc taatacctcc ccctccaggt
     3121 ccgtggcatc gctcaacaat cgaagggcct ggtctccgtg cgtcgcaatg aacacggcgt
     3181 cgaatcgctc gggggagcca tgcaaccctg tcacggtcac atagtcccgg tttcgtttga
     3241 tgctctctac cggggcattg gttcggatac gatgtcgaaa ggaagcggtc agtttgttga
     3301 cgtaggtctg ggctcctcca cgaacgacat accacgtcgg acgatcattg acgctcagca
     3361 tcccatggtt gtgaaagaac cgaataaaaa attgtgccgg catggcccac atctgcgagg
     3421 gatccgccga ccaaatggcc gcacccatgg gaatcagata ccgctcaaga aattctctac
     3481 cgtaacctgc ctgtttcaga tagttcccta acgtcgcggt catatccgga tttgcgaggc
     3541 actgggaggc ctcccgattg aaacgaagaa tgtcactgat catcctgtag aaagatggcc
     3601 ggagcaaatt ccgccgttga gcaaacagag tattcaagct cgtgccgtta tattccagtc
     3661 ctgatcgatc acatttgacg ctaaagccca tcgaggaggc ttgagctgga acatccagtt
     3721 ccttgagcag ccgcgtgaag cagggatagg tttggtgatt aaataccata aatccactgt
     3781 caagttggta cccttgtccc tcccactcga tgtcatgcgt gtgggaatgt ccaccgacgt
     3841 aatcattggc ttcaaagacc gtgatccggt ggtcgcggct gaggtgatag gctgccacat
     3901 ttcccgcaat gcctgtgccg ataatggcga ttttcatcgc gacagtctcc catgttcctc
     3961 ttccggcttc ggaagggtcg ccctacactc aaagactctc cccggaaacg ctcgaatatc
     4021 ctgacgcagg tcaaactgtt gaaatgcccg aggggttctt gtcgtgtttt ggatcaaagc
     4081 catatcttcc tcttcattgc atcaattctg tccgtctccc caccgttgtg gaacccgttg
     4141 aatttttgag aggtcataac cctgggcggc gatgatgttc agaagatgtt gataatcctc
     4201 ctccggcagc aggggggggc gggccatgat ccagacgtaa tcccgtttga gacggccgat
     4261 gatggtctga ctgtaatccg gattcacaaa cacgattcgg tagtcggcct tgaacggcca
     4321 gagaaattgc atgtcccaga cggcattgga ttcctgatcc acaacaaatc ctgtcgggcg
     4381 atacaccttc ctttcaccct cgaatccgcc tttgcgaaag gtaaaggtgg tggcgatggt
     4441 tccatcgtca ttgagtcgat aggactccac tgcgttgaat gcctccgtct caatgaacgt
     4501 cggaatgttc gcaatcacat accaatctcc catgaagcgt tcaagattga cctgactggc
     4561 gatctgaatg ggcggcgggc tgctgcaccc taacagactt ccgagcagcc cgcaggctat
     4621 caatgatgag gtgtgcattt tcctatttcc ctactgaagt tcatagcgca actttttgcc
     4681 gtctttagtg gtcgattgta gtccgaccca ttcgtcgtcc atggtgtacc agagatcaat
     4741 ggtaaacttg tcacttatga tgcggtgatg gtgagtgggg gtggtcactc cacgtactgt
     4801 gaatggttct tccgccacag tctgaatttc taccggctgt aattcgcccg tctgtgaatt
     4861 gagcaggcga ctgctctgaa accaggctgg attccagtag gcaaaggttt tgatacaacc
     4921 ctccaaggtt tgctcttcat catgggtgtg taaccgcaac gttccaccct tggaggtccc
     4981 ctccacaaaa aacgattcac cattgtcatt ggtcttggcc cgaatctcct tgaggcattc
     5041 cccttcccat acttcagcat tggtgtgccg gtaagtataa aacgtgatga aaaagtactt
     5101 tacatcgaac tcggcctcca cgttgatctg agtgcgcgtc cctttcgtgg acacgacaaa
     5161 gcgctgcaac ccgatttcat catcttccag aaagaccttg aaggaataag tcttttggtt
     5221 ggtggtctgg gcggatatgg ccagccctat ggagaaaata aaaaaggcca taagtgtcag
     5281 caaaagcgat gaaccttcga tcatggagaa cgttccaatc atgatatctc ctcctgatga
     5341 tcggctaatg agtttgtttc tttctgaggc cctggaatga agatgttggt gctcgccatg
     5401 tattcacgat aggctggtcg tcgatccaca attgtttctt cgagcatcgt cacaccggaa
     5461 aatttcagca ataaaaaagt taacaggata ggtgagagaa tcgaccacca agcgccggtt
     5521 gggatcacaa agaggaaaaa cccccaccag atcaggcatt ctccgaaata attaggatgg
     5581 cgtgtatagc gccacaatcc ttgattcatc accttgcccc gatttgcggg attcatcttg
     5641 aaacgagcaa gctgccaatc gcccaccgat tcaaagaaca ttccgattac ccacacggcc
     5701 acggccagag catcaaagat gctgtattca aatggcacgg ttaaggcgac ccacaatggc
     5761 atggagatga tccaggccaa cacggcttga aacacaaaaa tgatcccgag gctttttagc
     5821 gcaaagtttg gctcatactt gcgacgaatt gcctggtacc gggcgtcctc ggattcgccc
     5881 cagtttctcc aggtgatata caccgtcagt cggagggccc aaagaaggac gagggtgagc
     5941 accagggagg aacgattcca gtagggttcc acgctgcttg agtagacgaa cgcagacgcg
     6001 aagatcatga cggaccacat gctatccacg atactcacgt cccgtttgat gaggctgacc
     6061 agccaggtga gtgccgccag gacaagaatc gcgtgcaggc cggataggta gatggagaag
     6121 gtcatatggt gctttccttt agccttggtg caaagccatc aaaacgggtg gatagtgcca
     6181 tcaggattgg tgtgatgacg gcccagccca cggccagggc ggccaacgcc gcattttggt
     6241 taccaaactc gagggctccc aattggtgtc ctccataata ggccaatggt cctccaacgc
     6301 ttccggcaag tgcggctatc aaccagcgcc ctctcaacca acgaagtgag acgttcaaca
     6361 gggttgcgaa gagtccccac atgagcacga tccagtatgg ggcggtgtgt gggatgagga
     6421 taccggagga gtagtgcagc caatcgagcc acacgagcat gctgtcccat acggccccaa
     6481 tcaccagggc gatcataacc agtttgaatt ccgcttcagc tgcgggcgca ctgaacaggt
     6541 gaaaggccac aatgaccaga gccacgagtg ccccgatcca cgggcgttgg ccggctccgc
     6601 tgaggacgca ggcaaaccag ccgatctgaa acagtaaaaa gttgcagaga gtcctggcca
     6661 tgacttttcc cttcaggtga ggtcggcttt ctcaaaaaga taatggctga cccaccattc
     6721 ctgcccctcg cgatagccga ataattcggc acaggccata aaaaatatcc gccagcggat
     6781 ccaccagagg cccgcattcg gtttcccata ggtctggatg aacaatggcc ataaagcttc
     6841 tcggtggtgg tccatgtttt ccagccaggc attagctgtt cgttcgtaat gtgttccgtc
     6901 ccatctccag cgattcacga gtttgaacgg actttgaatg gccaaaggca gatcatcgct
     6961 gggcatcatg cctccgctga aaaaatgctc actcatccag tcgctggaat ctcgtacctc
     7021 gaatttataa ggcacggcgc gatgtacaaa gatgtgcata aagaaccggc catccggctt
     7081 gagccagcat tgaatgcgat cgaacaaatt ttcccaattc cgcatgtgct caaacatttc
     7141 cactgacacc acgcggtcga attgactgtg ttcaatgtga aatttattca tgtcgcatgt
     7201 gagcacgcga atattgcgca atccgcgctt ttgaacttga gagtcgatat acgctttttg
     7261 tgaatgagag tttgagactg ccgtgatatg actgtggggg tactgcgccg cgatacagag
     7321 tgtgagagat ccccagccgc atcccagctc tagaacggat tggccgtcat gaagatccgc
     7381 atgcaccttt gtctcccgta agctaacctc ctcagcttcg ggtagagact cgataccctc
     7441 cggccaaaat gcgctgctgt atttcagctg cggacccaac accttctgaa agaattctgc
     7501 tggaacttca tagtgttggg aattagcttt ttccggaaag ggtgctatgg aagacctgcc
     7561 aatgtcctta atgaaggata acttgtagga gggcagagcc tgttcgtcat aggaaatctc
     7621 tttcagtctc tggcggacca aatgctgaat gctataacgg agcaccgcat ccgggataac
     7681 accccggtca gttagtgagc gtgcaagtgt aaacaggatt gacataacct tttcttacgg
     7741 ggcatgagcc gaattcgcat tttatttttg aaattttaat caggctcata aaatttcctt
     7801 aatctcaata tattcctatg gattccagct atacaaaaaa acttaaacaa acattagaca
     7861 aactcgattt ttcaatttta cggacacatt tgaatagcgt cagtgtaggt gagggggtat
     7921 gttttgtagc gaaggaggaa ggaaaagtcc ggtgttcaaa cacgagactg tgcagcacaa
     7981 cgattgagct ggtaaggctg tggaacaaca gacgaggaaa ctattaggac gcgagagggg
     8041 gcgtgggccg atctccttac tatatggtgg aattatataa ccgacttggg aacgcctcat
     8101 tagccccagg atgtaggccc atggattttt gttgcaaggt atcttcgagc tttctttttc
     8161 acctcccctg aaaacagggg aagacctcga ttcttaattc gatggcgtta acgcggtcat
     8221 ttgtgatcat taggccaatt ccgaaaaggc ttccaacgct cgctcccggg aatcgctgag
     8281 tgtgacgatc ggcaggggat aatcttctcc gagttgcaca cccgcctcgg ccaacacttt
     8341 ctcaggcgct tcccatggat tatgcaggta tttcaccggc atctttttta attcggggat
     8401 gaaccgtcga acatactcgc cgttttcatc aaactttttt ccctgcgtga cgggattgaa
     8461 aattctaaaa aacggtgccg catcggcccc acagcctgcg atccattgcc agctggcact
     8521 gttgttggcc aaatcggcat cgaccaatgt atcccagaac cagtcttccc caagatgcca
     8581 gtgcaacatc aggtttttga ccagaaagga tccgacaatc atgcgcacgc ggttatgcat
     8641 atagccggtt tcccacagct cacgcatacc ggcatccacg atggggtagc cggtctggcc
     8701 ttgctgccat cttttcagag cttttggatc ttttcgccag ggaaagctat taaatttcgg
     8761 ttggagattt tttcggggga gttcggggaa atggaagagc agactgtggg aaaactcccg
     8821 ccagcccagc tcactcagga acgtttcccc gtctttccgc catccttccg tgctcatgcg
     8881 gtgtttggca gcatgccaca cctcattcgg tgagatttct ccgaaatgca gatgcgggga
     8941 taaccgcgag acattgtttc tggagggata gttccggcct tctttgtact ctttgaggcc
     9001 gcctttgaga aatgctttca gtcgggcctg tgccccggct tctccgggct cccaatgttt
     9061 cacaagtttt gtgtgccagg gaatttttgg gagcaggtct aaatctttca gtgcaacacc
     9121 tcggtgtgtg aaaagcctta aatcttttag ggctttttgg ggttgtcggg gttcactgcc
     9181 ctttccgaga catccccttt gataaaaggg ggtgaacacc ttataaggag tgccgtcgct
     9241 tttgagcgct gtctggggtt caaagagcag ggagccgtta aaggtgtgca ccgcgattcc
     9301 tttggtctgc aatgtctctt gaatgtgtcc atcgcgtgtg attcgccagg gctcgtaaca
     9361 tcggttccaa taaatcgctt ctgctcctac ctctttcatg acgtctatca gtaccttttc
     9421 agccgaccct gccttgaaac aaagcttgcc ctccaatgct tcgttcagcg ccttgagact
     9481 ttcatgcaac caccaacggc tggccgcgcc taacttccat tgtccgctat tttcctcatc
     9541 caaaataaat agcgggacga tagggtgacc tgtttgatgg gcggccaaca gtgccgggtt
     9601 gtccttaatg cgtaaatcct ggcgaaacca gacaattgcg agtgactcgt ttgtgggcat
     9661 gccttgagtc cttctcaccg ctccgaacag tggtgaacct gattctacat tgcctgtcat
     9721 tttttctcca tgcagaaggt ctttgtcaga tcatgaatgg ttttctttaa aattcctaag
     9781 gcgaccatgt ccccagagaa gagccttggt ttttttacag atgagaaaat gacggctcat
     9841 gattttttca acggatgagg aattgtttct aaatctctcg tgcgaaatgt caggagacgg
     9901 ggagacgtat attggtcaat aattcgtaaa gcctcatttc gctccttttc gtcagacaac
     9961 gtccccagat agcggttctt taattcttct tcgggtttcg gcataagcgc atagctaatt
    10021 gtgacctcga ttaaagccgg agtggaacct tccgggactt taatgtaaaa gagaagatgg
    10081 cgtgtgattc cgctattgac caagggttgc ggtatgggtc ctcttaatat gccttcaaat
    10141 gagcgcccaa aggttttgta ctcttcgcca atgattgttc cggtgttgtc cttcacaatg
    10201 aatttcagga aaaattggtt gaaaattggg tctccaccgg ggaacatgtg aatcaagctc
    10261 ccattgggta aatgaagagt gccctgaacc gaggatttgg tctgtgtgct ctcgatatcg
    10321 aggcgtgatg tccattctgc ctgtacgttg cgattactca acatgattcc cggtgccacg
    10381 actccctgaa accagtgacg tgccagtgat ctggaatgtg tactcccctt gtcagttgaa
    10441 ctgccatacg attgttccat gcggcaattt tgacaaacca agtccttcag gatggctccc
    10501 tctagatggg actgcgtaat atccttgacc ttagtgaagt gacaggaagc acagaaatcc
    10561 gctcctttat agatctccgc ctgctgtgat tgatgggcca ggttatcctc cgcattgccg
    10621 tatgggccga aaaagatggg gccaggattc agttgaaatg ccgggtaagg accctccttg
    10681 atgttggaag tctgaatgag atgacaggac gcacatccga tgccctccac tttgacgtgt
    10741 ttcggtcccc tcatgacttg gtcaataatg cgatccgcat attgtggaaa gacggtgacg
    10801 gctggaacat gacaagccag gcatctcgtt cgttgttcat gattgggttg ggtttgaagc
    10861 cacaaaccaa gcacagtcct gaagacagga gacttcaggg ctgtcccatg cggcaaagct
    10921 ccctccatcc gtccaaacga atgaagatcc tcgctacgct ccctcatgcc tttccattct
    10981 tcaaaatgtc ggtcatggca ttgcttgcat cgagttgagg ggtaaagatc gtctcaattt
    11041 catcgagcca tgtgcgatcc tttttcgatt gagacttttc ctgtgaccat ccctggctgg
    11101 aggagagttc cgccaccggt agcgatatac ttagaacggc catgagcaag aaaagcaatt
    11161 tggtgggaag tttgttgaca accataaacc ttagccgaaa aatctgtgtg ctggactgtt
    11221 gaggagcacc gtttaaagta tgtttgtgcg ggtatttatt cattcctatc atatcgtgcg
    11281 ttgtgggagc ataaacaatc aaaatcctaa aaccttcgac aaataatgct ggaaaatttt
    11341 atcccttttc cggtggccac aggcatcaat cgatgcacca tcggatggtg aaagttggca
    11401 atatttaaaa agtattcagg atcaccagat ggtaaaaaca tctaacattg gacaaagttt
    11461 cggtgtcgac catcacctaa tgttaatgtc tgtcttggga tctgtcggga ctgtatccgg
    11521 cgatgaataa cactcaaggt aatggaataa gaaaaaaccg gagaggagag cgtgaggcac
    11581 gcgagctatc tcaatagggc tctgggggga ttgatgatag gacttgttgg gtggctggga
    11641 ggatgtgacc ttccagagcc gcctaaggca gccgaggcta tcccatctgt ctacgcgaat
    11701 ttacacatgc cggatggctg gtggaccgac cctacaatta ttgaggatgg tcgtcagttg
    11761 tatctcggtt tgaataaatc caatgtcaac tgcgcacaat gccatggtaa aaccggcaaa
    11821 cccgtaatga ccgcagcttt gagcttcctc gatgtggata agatgaaaag gtattcggat
    11881 tcccagatgt tgtggcgaat ttcggaggga attccctaca ctagaatggg agctcataag
    11941 gataagctct ctcaagacga aatctggaaa atcatcgcat ttgtgagcac attgggattg
    12001 gacggttggc aatatgatcc caattcgaaa agttgggttc ccaatgaggt gcgtccgtga
    12061 gaaaatcttt gaggtctatg aaaaggtcaa aatatgtcct gtgctcctat gcgccactcg
    12121 acctactaaa gcaggtttag tggtggatgg gttcagcatt aatattttct tgtatcaacg
    12181 atccttttgg ctcatcgagc atggaccgcc cactataaaa ataggtttct gattgtaagt
    12241 tcttttcgag tgaagtctgg tgacgaaata agtttattca aggggacatg atctatcgtc
    12301 ttatgtttgt gataaaaaac gcgctgcctc tccctttgtg taccttatag aaattttcga
    12361 taagggagtg gtcccatttg gcctaccata attcagtgcc cggttcgcct ttgaagggac
    12421 cgaggatttt cgaggtgatc catccgccat aaaattcacc ggactgtggt ttgacctttt
    12481 gcttccccac atagcagcca tccacctttc cgggataaaa cgctaaataa ttggaaatat
    12541 actcatatcc tttgccggga tgtgggtaac tccatgcggc attttgcgcc attcttttcc
    12601 caatcccaat tgaccaataa cgcgccattc ctttccactc gcatacggtt tcccttacgc
    12661 tcagcatcag gtaggcgatt cggacatcag tggggggaaa ata
//
LOCUS       DATIAL010000219         6980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_468795, whole genome shotgun sequence.
ACCESSION   DATIAL010000219 DATIAL010000000
VERSION     DATIAL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6980
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468795"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..78
                     /locus_tag="VLA60_06880"
     tRNA            4..78
                     /locus_tag="VLA60_06880"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:cac)
     gene            260..1441
                     /locus_tag="VLA60_06885"
     CDS             260..1441
                     /locus_tag="VLA60_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09120.1"
                     /translation="MLQPINHHYRLHAKPTNLLKVFLLFFLLPHCASAPPASNLPPSE
                     SDLNLLGKAALCQTKTEAQTSWLQPVGQESPWGGGTEVFRETASPKKHAQWFMFNEDQ
                     TLVGVITVFPRGLALEDYPKLRHTLSQLPPAREFFFHSSQLLKEQTPDSGTLHRTGEA
                     TTTHQYFMRHTQGKQDQLVMAVFVLDPYETLLDGSHSKFLSYIEDPDPPDKDLTGTIG
                     QFGSEKEFLGLQQFARGEIALFASCGNKQPELAVDAYQKAIRYGLSDPKQLGEAHHRL
                     GLALRSMGRLSEASTALEQALTIQPYSAVILNSYGSVLTQLGNVPKAIETYERALSLQ
                     PNYAQARFNLAEAYEAHNPKRAIQEYETFLILAGDNPDEMTKIDLAKARIKALQGGAR
                     Q"
     gene            1544..2164
                     /locus_tag="VLA60_06890"
     CDS             1544..2164
                     /locus_tag="VLA60_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HSF09121.1"
                     /translation="MKFYAVRRGRTIGIFESWDACRAQVHHFPGAEYKAFTNREDAEA
                     FLLSRSSSQPIKKKRTTTATSSSLIEVWVDGSCLPQGDGSLRLGWGVLVKKNGVEIHR
                     DKGNDIPQAAMNHRNVAGEILAILKAIKWCQSEGITEMTIYFDYQGLESWATGAWRTK
                     LPFTQAYAQAVNESGITIHWVKVKAHSGNPENDLVDQLAKEGARGE"
     gene            complement(2227..3213)
                     /locus_tag="VLA60_06895"
     CDS             complement(2227..3213)
                     /locus_tag="VLA60_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09122.1"
                     /translation="MSTTEPSQTEHEIPWTEEASSRLERAPLFLRGMVRRLAEKKAKE
                     LGYTEITAETLDQFKQQMMGSMGGASGMTQAAEDMAQGKLPWTAEARKRLEAVPEFMR
                     SMIGRIAEDVAKERGHMEVNVELFEKVEALGDIPIEKSIPMEWTDEALALLNKRIQDS
                     PPIAMEFVTDMLKRDAEDLAREQGIAKIDAETLIRLWEDPPTQVAWTDEAWKRLHTSP
                     DFVRSGIRKAAERRARKLGLTTIDSEHLTTFRNEAMMKAVKRIRSFGYQELTFDAFGD
                     AMQKVKRLKGNEQAEHRLDEIRDYMKKKPDVGVLGDELMTRFRNYLKGEGKL"
     gene            complement(3217..3510)
                     /locus_tag="VLA60_06900"
     CDS             complement(3217..3510)
                     /locus_tag="VLA60_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HSF09123.1"
                     /translation="MTKADLIEKLAEKAPQLSRRQAELVVNTIFDSIRDSLKRGEKTE
                     IRGFGSFRLRHRQTKEGRNPKTGESVSVPEKRMPFFKAGKEIKELLNPELPSV"
     gene            complement(3611..4489)
                     /gene="sppA"
                     /locus_tag="VLA60_06905"
     CDS             complement(3611..4489)
                     /gene="sppA"
                     /locus_tag="VLA60_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSF09124.1"
                     /translation="MSNGYPRWKRVLWMILGGLVLFSVGKSLIPELLLAGKDSVAIVR
                     VEGPILDSYQTVEELKKFADDPLVKAIVVRIDSPGGGVAPSQEIYNAVKRVRKEKNKT
                     VIASMGTVAASGGYYIAVGTDRILANPGTLTGSIGVIMQLANFHELLEKIGVKNLVVK
                     SGKYKDIGSPFRPMNDEDRKVLELVMNDVHRQFIDAVADGRSLDVAEVEQLADGRVFT
                     GQQAKSILLVDDIGDLHDAIKLAGELGGIEGEPRVMEVRKPFSFRDLLENTFLGSVRT
                     FATIHFPTPLLYLWVA"
     gene            complement(4547..6250)
                     /locus_tag="VLA60_06910"
     CDS             complement(4547..6250)
                     /locus_tag="VLA60_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HSF09125.1"
                     /translation="MNTLTQQPEKIDRETLAALYDETFKNIEEGTITEGRVIDIQRDR
                     VVVDIGYKSEGMIPADQFSPEELEQLAVNDPVQVYIEQCEDADGNLLLSKEKADKMKV
                     WEDLEVAHKDEKQVQGKVISRIKGGMIVDIGVKAFLPGSQIDLTPVRDLDGLVGKTYN
                     FKIIKINHRRGNVVVSRRALLEETRDKRRQSTLSTLSEGQLIEGTVKNITDYGAFLDL
                     GGIDGLLHITDISWGRVGHPSDMFSIGDRVEVLVLKYDRETGRISLGVKQKSPDPWTK
                     VEEKYPARSRVKGRVVSLTDYGAFVELEPGVEGLVHVSEMSWTHEVRHPSKVVSVGDT
                     IEAQVLHVDQHSRKISLGMKQTAPNPWDIIEDKYPEGTEIEGKVKSVTDFGAFVGLDE
                     GVDGLIHISDMSWTKHIKHPSELFKKGQSVKAVILKIDKEKERLSLGYKQLISDPWVS
                     EIPQQYHVGEVTTGKVSKIADFGVFVELEGGVEGLIHISEIGPDVQERLEEKFAVGQE
                     VAAKIVKVDCDDRKIALSLWEHLKDLEQTEVEEFNSSQGSVDQSFGRVVQKKDQGDEG
                     E"
     gene            complement(6305..>6980)
                     /locus_tag="VLA60_06915"
     CDS             complement(6305..>6980)
                     /locus_tag="VLA60_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSF09126.1"
                     /translation="SFVSGVVYGLLWILFNGLARLLFRFRTEGSQHFPKTGGIIVAAN
                     HASYFDIPLLGCAIPRRVFFLGRASLFPNRYLNWALQKLGWIPLKTHRLDRKAFGQAL
                     SHLQAGEPVVIFPEGTRTEDGSLQVGKPGLGYLVAESQCQVIPVYISGTFTVLPIQAR
                     WPRLFPLSVSFGKPLKFCKDEGKSVKSFYEDVSITVMDHIAQLGGVPSPSQQAYEKDS
                     RSKLCE"
BASE COUNT         1838 a         1941 c         1407 g         1794 t
ORIGIN      
        1 cgcgggcgat tagctcagtg gaagagcgct tccttcacac ggaagaggcc actggttcga
       61 tcccagtatc gcccaccatg atacaccttt ctggatcaat tgcttcccgg cactatcgtt
      121 gttcacatcc gaagcacata aaagcgagaa cttcttgatc gtgcatttat tttttaccca
      181 cgacctaatt cggactccat tatcctaatc gcagttattc tacgacacat cgatcattta
      241 tcctcaagag ccggtagtaa tgttacaacc gatcaatcat cattatcgac tccacgcaaa
      301 gccgacaaat cttctcaaag tgtttttgct ttttttctta ctcccacatt gcgccagcgc
      361 tccccctgcc tccaaccttc caccttccga aagcgacctc aacctcttag gaaaggccgc
      421 cttgtgccaa accaaaacag aggctcaaac gagttggctg caacccgtgg gacaggaatc
      481 tccttgggga ggaggcaccg aagtctttcg tgaaaccgca tcacccaaga aacacgcgca
      541 atggttcatg tttaatgaag atcagaccct tgtgggcgtc atcaccgtat tcccacgcgg
      601 tctggcttta gaagactatc cgaaacttcg tcataccctg agtcaattac ccccggctcg
      661 tgaattcttt tttcattctt cccagcttct aaaagaacaa acccccgatt ctggcaccct
      721 gcatcggacc ggcgaagcca cgaccacaca tcaatatttc atgcgacaca ctcaagggaa
      781 acaagaccaa ctggtcatgg ccgttttcgt tcttgatcct tatgaaacgc ttttagatgg
      841 cagccattca aaatttcttt cctatattga agatccagat cccccggaca aagacctcac
      901 gggcacaata ggacaattcg gatcagaaaa ggaattttta ggacttcagc aatttgccag
      961 aggagagatc gccttatttg cgtcgtgtgg caacaagcag ccagaactag ccgttgacgc
     1021 ctatcagaaa gccattcgat atggcttatc tgatccaaaa cagttagggg aagcccacca
     1081 tcgtcttgga ttggccttac gaagcatggg cagactttca gaagcctcaa ctgccctgga
     1141 acaggccctg accatccagc cctactcagc agtcatcctc aatagttatg gctcagtcct
     1201 aacgcaatta ggaaacgtgc ccaaggctat cgaaacctat gagcgggctt tgtccctgca
     1261 gcccaactat gctcaggccc gattcaattt ggctgaagct tatgaagcac ataaccccaa
     1321 acgcgccatt caagaatatg aaacattcct gatattggca ggggacaatc ctgatgaaat
     1381 gacaaaaatt gacctggcca aagctagaat taaggcccta caaggcggag ccagacagta
     1441 atcaaactaa atttaacttt tatgtgacat accccgttca agcactgttc atatcaaaag
     1501 ccctttgtag tgaagtagtg aataatccac gcattaagcc ttcatgaaat tctatgccgt
     1561 ccgccgcggc cgcaccattg gcatctttga gagttgggat gcctgccgtg cccaagtaca
     1621 tcattttccc ggagccgaat ataaagcctt caccaaccga gaagacgcgg aagcttttct
     1681 gttatctcgt tcaagcagtc aaccaatcaa aaagaagcga acaaccacag caacatcctc
     1741 ctcgcttatc gaagtttggg tggatggatc ctgcctccct cagggcgatg gctctcttcg
     1801 actgggatgg ggggtactgg tcaaaaaaaa tggtgtagaa attcaccggg acaaggggaa
     1861 cgacattccg caggcagcca tgaatcaccg aaatgtcgct ggggaaattc ttgccatcct
     1921 caaagccatc aaatggtgcc aatccgaagg gataacagaa atgacgatct actttgacta
     1981 ccaaggactg gaaagttggg caaccggagc ctggcgcacc aaactaccat ttacccaagc
     2041 ctacgcacag gccgtcaacg aatcaggcat cacgattcac tgggtcaaag tgaaagctca
     2101 ttccggcaat ccggaaaatg acctcgtcga tcaacttgcg aaagaagggg ccagggggga
     2161 ataagacagg ggaagttcac gagggtttcc aacctcacaa ccagaagagg acgaatcgaa
     2221 tatcagttac agttttcctt ctccttttaa ataattccgg aaacgggtca tcagttcatc
     2281 acccaataca ccaacatcag gctttttctt catgtaatcc ctgatctcat ccaaacgatg
     2341 ttccgcctgc tcattcccct ttaatcgttt aactttttgc attgcatcgc cgaacgcatc
     2401 aaaggtcagt tcctgatagc caaaacttcg aatccgcttg acggctttca tcatcgcctc
     2461 atttcgaaat gtggtgaggt gttcggagtc gatagtggtt agcccgagtt ttctcgcacg
     2521 gcgttctgcg gcttttcgaa taccgcttcg gacaaaatcc ggtgaggtat gaagacgttt
     2581 ccaggcctca tccgtccatg ccacctgtgt gggcggatcc tcccagagac ggatcaaggt
     2641 ttcagcatca atcttcgcga tcccttgttc cctggctaga tcctcggcat cccgctttaa
     2701 catatccgtc acaaattcca tagcaatcgg cggtgaatcc tgaatacgct tattcaataa
     2761 agccaaagct tcatcggtcc attccatagg gatgcttttc tcgatcggaa tatcccctaa
     2821 ggcttcaacc ttttcaaata attccacatt cacttccata tgaccgcgtt ctttagccac
     2881 atcttcagcg atcctgccaa tcatgcttcg cataaactct ggaacggcct ctaggcgctt
     2941 ccgcgcctcg gcggtccagg ggagtttacc ttgagccatg tcctcggcag cttgagtcat
     3001 gccggacgcg ccccccatcg agcccatcat ttgctgctta aattggtcta aagtttcagc
     3061 cgtaatttct gtataaccca attctttggc ctttttttct gcaagacggc gcaccatgcc
     3121 tctcaggaac aaaggagcac gttccagtcg agaagaagcc tcttccgtcc agggaatctc
     3181 gtgttctgtt tgagatggct cggtagtaga catgatttag acagatggta attcagggtt
     3241 taacaattct ttgatctctt taccagcttt aaaaaacggc atgcgctttt cagggaccga
     3301 caccgattct ccagttttag ggttacgccc ttctttggtc tgacggtgtc gaagacgaaa
     3361 acttccaaaa ccccggattt ctgttttttc tccacgcttt aaagaatcac ggatagaatc
     3421 aaaaatggtg ttgactacta actcggcttg ccttcgactc aattgaggag ccttttccgc
     3481 caacttttcg atgagatccg cttttgtcat ccgatcctcc ttctcacaga gactcgcttc
     3541 ttcaaccttg aattatgcca atgagcatcg cttctcaaaa ggcgcaatta atccattttg
     3601 ctaatccgga tcaagccacc caaaggtaca gcaacggggt gggaaaatga atcgtggcaa
     3661 aggtgcgaac gcttccaagg aatgtgtttt caagtaaatc cctgaatgaa aacggctttc
     3721 tcacctccat gacccgtggc tctccctcaa taccgcctaa ttcgccggct aatttaatgg
     3781 catcgtgcaa atcaccgata tcatcaacta acaaaattga cttggcctgc tgccccgtaa
     3841 agacacgccc atcggctagc tgctcgacct ccgccacatc cagggatcgc ccatcggcca
     3901 ccgcatcgat aaattgacga tggacatcat tcatcaccaa ctctaagacc ttgcgatctt
     3961 catcattcat gggacgaaaa ggagagccga tatccttata ttttccactt ttgacgacaa
     4021 ggttcttaac accgattttt tccagtaact catggaaatt cgccaattgc ataatgacgc
     4081 caatactccc cgtcaaagta cccggattag cgagaatgcg atcggtgccc acggcaatgt
     4141 aatagcctcc agaagccgcc accgtgccca tggatgcgat caccgttttg ttcttttctt
     4201 ttctcactct cttaacggca ttataaattt cctgagaagg cgccacgccc ccacctgggc
     4261 tatcaattct caccacaatc gctttgacca aaggatcgtc agcaaatttt ttcagttcct
     4321 cgacagtctg atacgaatcg agaatgggac cctcaacccg aacgatggcc acactatcct
     4381 tacctgctaa caataattcc ggaatcaggc ttttcccaac actaaacagg acaaggcctc
     4441 cgagaatcat ccacagcacg cgtttccatc gaggatatcc attactcata atattcggga
     4501 ttggccataa gtcccgcgct catcaaaacc atcctgccca tgaagcctat tcgccttcat
     4561 ccccttgatc tttcttttgg acaactcgcc caaagctttg atcaacactg ccttgagagc
     4621 tattaaattc ttccacctcg gtttgttcta gatccttcaa atgctcccaa agacttaacg
     4681 cgattttccg atcatcgcaa tcaaccttga cgattttggc ggcgacttcc tgaccaacag
     4741 caaatttttc ctcaaggcgc tcctgcacat ctggcccgat ctcactgatg tgaatcaacc
     4801 cttctacgcc gccttccaat tccacgaata ccccaaagtc ggcgattttg gaaacttttc
     4861 cggtggtgac ttcgccaaca tgatattgct ggggaatttc agagacccat ggatccgaga
     4921 ttaactgctt ataccccaaa gataaccgtt ctttttcttt atcaattttt aaaataacgg
     4981 ctttgaccga ttgccccttc ttaaacagct cggaaggatg cttaatatgc ttggtccacg
     5041 acatatcaga aatatggatc aacccatcga ccccttcatc taaaccgaca aaggcgccaa
     5101 aatcggtgac actttttact ttgccttcaa tctccgtgcc ttcagggtat ttgtcctcaa
     5161 taatatccca tgggttagga gcagtttgtt tcatcccaag agaaattttc cggctatgtt
     5221 ggtcaacatg gagcacctga gcttctatgg tatcgcccac agacacgacc tttgacgggt
     5281 ggcgaacctc atgggtccaa gacatttccg acacatgaac caaaccctca acgcctggct
     5341 ccaattcgac gaacgcacca taatcggtca ggctcacaac tctccccttc acacgacttc
     5401 ttgccggata tttttcttca acttttgtcc agggatctgg agatttttgc ttaacgccca
     5461 aggatatacg acccgtttcc cggtcgtatt tcaatactaa gacttcaact cgatccccaa
     5521 tggagaacat gtccgatgga tgccccacgc gaccccagga aatatcggta atgtgtaaca
     5581 gaccatcaat gccgcccaag tccaagaacg ctccgtaatc ggtgatgttc ttcacagtcc
     5641 cttcaatcaa ctgcccttca gacagggtcg ataaagtcga ttgtcttcgc ttatctcggg
     5701 tttcctcgag gagcgcccgt ctggagacca ccacattgcc gcgacgatgg ttaattttaa
     5761 taattttaaa gttatacgtt ttcccgacca aaccatccag gtctctgacc ggcgtcaaat
     5821 caatttgcga tcctggcaaa aaggccttca ccccaatatc aacgatcatg ccgcccttaa
     5881 tacgagaaat gactttccct tgcacttgct tttcatcttt atgggcaact tccaaatcct
     5941 cccacacctt catcttgtcg gctttttctt tggagaggag caaattgcca tccgcatctt
     6001 cacattgctc gatatacact tgaaccgggt cattcaccgc caactgttcc agctcttccg
     6061 gagaaaattg gtcggcaggg atcatgcctt cagacttata gccaatatcc accaccacac
     6121 gatctcgctg tatatcaatg actctgccct cggtaatggt tccttcttca atatttttaa
     6181 acgtttcatc gtataatgcg gcaagagttt cgcgatcaat tttttcaggc tgttgagtaa
     6241 gagtattcat ccgacttttg atacctccgg gagagtttgg cgggttaaat ggtgattgcc
     6301 atgattattc acacaacttg cttcttgaat ctttttcata cgcctgctgg gacggagatg
     6361 gaacaccacc caattgagcg atatgatcca taaccgttat cgatacgtcc tcataaaatg
     6421 acttgacact tttcccctca tccttacaaa atttcaatgg tttcccaaag ctgacgctta
     6481 acggaaacag ccgcggccat cgagcctgaa tgggaagaac tgtaaaggta ccagaaatat
     6541 acacaggaat cacctggcat tgtgattcag ctaccaaata acccaggcca ggcttcccga
     6601 cctgtaagga cccgtcctcc gttcttgttc cctcagggaa aataactacg ggttcgcctg
     6661 cttgcaagtg agagagtgcc tgtccaaagg ctttcctgtc cagtctgtgc gtttttaaag
     6721 ggatccagcc gagcttttgc aaggcccaat tcagataccg attgggaaat aagctcgctc
     6781 tccctaaaaa aaaaacccgc cgcggaatcg cgcaccctaa caggggaata tcaaagtagc
     6841 tggcatgatt tgccgcaacg atgatccctc ctgtcttggg aaagtgttgg gaaccctccg
     6901 tccgaaatcg aaacaggaga cgcgccaacc cattaaacag gatccataac agcccgtaga
     6961 ccactccact cacaaacgat
//
LOCUS       DATIAL010000220         4632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1221317, whole genome shotgun sequence.
ACCESSION   DATIAL010000220 DATIAL010000000
VERSION     DATIAL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221317"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VLA60_06920"
     CDS             <1..873
                     /locus_tag="VLA60_06920"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSF09127.1"
                     /translation="VAEVQRIIGHLFWLGTQALDIGAMTVFFYTFRDREILLDWFDEL
                     CGARLTTSWYRIGGVERDFTPSLTEKVQRFLDYFPPKIDEYVVFLEKNRIWLARTRGV
                     AVISAEDALSFGLSGPTLRGSGVNYDLRKYEPYSAYPKCEFSVPVGKNGDTYDRYWIR
                     IEEMRESLKIIQQCLQQMEPGPILADVPSVTLPPKDRVFTNLESMIQQFKLFSQGFDA
                     PAGEIYCGTEAHKGELGFYIVSTGGGRPYRLKIRAPSFIHMGAFDYMAKGYMIADAVT
                     IFGTYDIVMGECDR"
     gene            935..3625
                     /locus_tag="VLA60_06925"
     CDS             935..3625
                     /locus_tag="VLA60_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF09128.1"
                     /translation="MGLKPVTTPDVEAATIELTLDGEVVQAKEGVSLYDVISSTGKIV
                     PAMCYHYTFDPFGSCGMCLVFQEGKKAPVRSCTAKAAAGMVIQTEGEDLFLARKKAVE
                     KHLSVHPLDCPVCDADGHCELQDMAFQHGVTNLANAKQKNIPEDTRSLVLDFNMNRCI
                     ACGECINICKDVLRIDALQFMKKGGFTQVVAKGDQALDCEFCGDCLAVCPVGAITNKF
                     SKYAYKPWQLKKTTTTCNYCGDGCQIYVETKDTEVVRVTSPLSWKNKWGDRTDTVNGH
                     DGICVRGRFGFQFIDSDTRLKTPLVRTESGLQPSPWLDALDLAAGRLAYVKAQYGAQA
                     IAGLITARCTNEDLYVFQKFMRSVIGTNHLDSSARYGHLNFVRAMKHALGLHRMMNTS
                     EEINKAKAVLIIGANITETNPVASLRVKSAMGLYKAQTIVVDSMQTNIAKLASHPMMV
                     KPGTEGLFVQGLVKSVLDQDLIDEEATSAFPEALHALRQAVGLLSLEDMASQTGVGLE
                     QIHEAARIFAEASRSIIICGEGILRHAGGYQHMLNIIDLAWVTGKLGRKGCGINTYTE
                     EANEQGAVDMGVAPEYLPGPVSFTDSQARETFARVWGNNLPDVDRGANLTEILDRCQN
                     GEIKALYLVGENPLETLPASYDVTSALAKLEVLICQDPFLTEIAKMAHVVFPASTFAE
                     KDGTVTNQEGKVQFLRPALDSLGESTLDWHIMVGMANGLGSPMEYETTQDIQNEIRKI
                     LPRYYNLGKVSHVAPDLSLYFSKAFPEEVASRYPVGMKSPTQRPFALRMIQLLYHSGK
                     LSTQASGLMEISPNTKRLRMSAEDFNNLALSAHDRVRITADQGTLEMGVEEDTSLMPG
                     TCTVPEHFNDPPVKDLMSVQVDPVTGVPYFKVTYVSIEKA"
     gene            3649..4218
                     /gene="nuoI"
                     /locus_tag="VLA60_06930"
     CDS             3649..4218
                     /gene="nuoI"
                     /locus_tag="VLA60_06930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HSF09129.1"
                     /translation="MSNSPATKKFFDAVLFREIWDAMKVTLKHLLHRPITFQYPREKR
                     TIPDAHRGALCLLRYEDGKERCVGCDLCEAACPSRCIKVLSEEDKTLPLQRYATEFYI
                     DITKCVFCGYCVEACPVNALAMTKVYEFSTHDKRTLLFDKARLYQIGERHIDDAKKYL
                     YAHNQEKEGEEGRAYRYHFPVSIKSTQEP"
     gene            4226..>4632
                     /locus_tag="VLA60_06935"
     CDS             4226..>4632
                     /locus_tag="VLA60_06935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HSF09130.1"
                     /translation="MMWLLFGYFATVSVVAGVLTVALRNAVHCALALLTLLLHVAGLF
                     VLLNAEFLFAVQIIVYAGAILILYLFVLMLMNLKTEEQHLHKKYAFLVFAGTAIFGEL
                     LILLLQSPYGGVIGAATPDLILQTGPSHAIGIT"
BASE COUNT         1124 a         1064 c         1231 g         1213 t
ORIGIN      
        1 gtggctgaag tgcagcgtat tatcggccat ttgttctggt tgggtaccca ggccttggat
       61 atcggggcaa tgaccgtgtt cttttatacg tttcgtgacc gggagatttt gttggattgg
      121 ttcgacgaac tgtgtggcgc aagactgaca accagctggt atcgcattgg cggtgtcgaa
      181 cgcgatttca ctccttcgtt gacagaaaaa gtccaaaggt tcctggacta ctttcctccc
      241 aagattgacg aatatgtggt gtttttggag aaaaaccgca tctggttagc ccggacacgc
      301 ggtgtggcgg tgatctctgc cgaagatgcc ctgagttttg gattgagcgg tccgaccttg
      361 agaggatccg gggttaatta cgatctgcgg aaatatgaac cctatagcgc gtatcctaaa
      421 tgtgaattta gcgtgccggt tggaaaaaat ggagacacct acgatcggta ctggatccgg
      481 attgaggaaa tgcgtgaaag ccttaaaatt attcaacaat gcttgcagca aatggagcca
      541 gggccgattt tggctgatgt gccgagtgtg actttgcctc ctaaggatcg ggtgttcacg
      601 aatctggagt cgatgattca acaattcaaa cttttttcgc agggatttga tgcaccagcc
      661 ggggaaattt attgcggaac tgaagcccat aaaggcgaac tgggatttta catcgtcagt
      721 actgggggcg gtcggcctta ccgtctgaaa attcgtgctc cctcatttat tcatatgggc
      781 gccttcgatt atatggccaa aggctatatg atcgccgatg cggtcaccat ttttggcaca
      841 tacgatattg tcatggggga gtgtgatcgg tagtgtggtc tttccggact tcgtcttgaa
      901 ttatggtatt ggctacagtt aaaaaaggac ctcaatggga ttaaagcctg tcactacacc
      961 tgatgttgaa gcggcgacga ttgaactgac cctggatggt gaagtggtcc aggccaagga
     1021 aggggtctct ctctacgacg tcatatccag cacgggtaaa attgtgcccg cgatgtgtta
     1081 ccactatacc ttcgatccct ttggatcctg tggcatgtgt ctggtttttc aggaggggaa
     1141 gaaagcacct gtccggtcat gtaccgcgaa ggcggcagcc gggatggtca tccagaccga
     1201 gggggaagat ctgtttctgg ccaggaaaaa agctgtggag aagcatctct ccgtccatcc
     1261 gctggattgt ccggtgtgcg atgccgatgg ccattgcgaa ctgcaggaca tggcctttca
     1321 acatggcgtg accaacttag ccaacgcgaa acaaaaaaat attccggaag atacgcgcag
     1381 cctggttctt gatttcaaca tgaaccgctg cattgcttgc ggagaatgca tcaatatttg
     1441 caaagacgtg ctacgcatcg atgccctgca atttatgaaa aagggcgggt ttacccaggt
     1501 agtggcgaaa ggcgatcaag cgctggattg tgaattctgc ggtgattgcc tggcggtgtg
     1561 tccggttgga gccatcacca ataaattctc gaaatatgcc tataaaccct ggcagctcaa
     1621 aaaaacgacc acgacctgca attattgcgg cgacggttgc caaatttatg tggaaaccaa
     1681 agatacggaa gtggttcggg tgacctcccc cctttcctgg aaaaacaaat ggggagatcg
     1741 cacagatacg gtaaatgggc atgacggtat ctgtgtgcgt ggccgctttg gatttcagtt
     1801 cattgatagt gacacccgtc tgaaaactcc attggttcgt accgaatccg gattgcaacc
     1861 ttccccctgg cttgatgcat tagatttggc tgcaggtcgt ttagcctatg tcaaagcgca
     1921 atatggtgcg caagccatcg ccgggcttat tacagccagg tgtaccaacg aagatctgta
     1981 tgtgtttcaa aaatttatgc gttctgttat tggcaccaat catctggaca gcagtgccag
     2041 atatgggcat ctgaattttg tcagggccat gaagcatgcc ttgggccttc atcgcatgat
     2101 gaatacctcg gaagagatca ataaagcgaa ggccgtcctc atcattggag ccaacattac
     2161 agagaccaat ccggttgcaa gtttgcgggt gaagtcggct atggggttgt ataaggctca
     2221 aaccattgtt gtggattcca tgcaaaccaa tatcgcgaaa ttggcctctc atcccatgat
     2281 ggtgaagcca ggtaccgaag gcctctttgt tcaaggtctg gtgaagtcgg tgctggatca
     2341 ggatttgatt gacgaagaag ccacgtcggc atttcccgaa gccttgcatg cgttaaggca
     2401 ggcagtcggg ctactttctc ttgaggacat ggccagccag actggagtgg gtctggagca
     2461 aattcatgag gccgctcgaa tttttgctga agcgtcccgg tcgatcatta tttgcggtga
     2521 gggtattctc cggcatgccg ggggctatca acatatgttg aatattatcg atctggcctg
     2581 ggtaacagga aagcttggtc gaaagggatg cggcattaac acctataccg aggaagcgaa
     2641 tgaacaaggg gcagtcgata tgggtgtcgc accggaatat ctgcccggtc cggtttcctt
     2701 cactgattcc caagcccggg aaacatttgc tcgtgtctgg ggcaacaacc ttccggacgt
     2761 tgaccgcgga gccaatctca cggaaatcct ggatcgttgt caaaatgggg aaattaaagc
     2821 cctctatctg gttggcgaaa atccattaga gacgcttccc gcctcctatg atgtcacaag
     2881 tgcattagcg aagttagaag tcctcatttg ccaggacccc ttcttaacgg aaatcgccaa
     2941 gatggctcat gtggttttcc ctgcttctac atttgcggaa aaagacggca cagtgaccaa
     3001 tcaggaagga aaagtccaat ttctccgtcc ggctctggat tctcttggcg aaagcacctt
     3061 ggattggcac atcatggtcg gaatggcaaa cgggttaggc tcgcccatgg aatacgaaac
     3121 gacccaggat attcaaaatg aaatccgaaa aatcctgcca cgttattaca atcttgggaa
     3181 ggtctctcac gtggctcctg acctttcgct gtatttctcg aaggcttttc cagaggaagt
     3241 ggcgtcgcgg tatcccgtag ggatgaagag tcctactcaa cggccttttg ccttacgcat
     3301 gattcaattg ctttatcatt cagggaagct ttctacccag gcatccggtc tgatggaaat
     3361 ttcacccaat acgaagcgtc ttcgcatgag cgccgaggac ttcaataatc ttgcactcag
     3421 tgctcatgac cgtgtgcgga tcaccgccga tcagggaaca ttagagatgg gagtggaaga
     3481 agatacgtcg ttaatgccgg gtacctgtac ggttcctgaa catttcaatg atccacccgt
     3541 gaaagacctg atgtcggttc aggtcgatcc tgtcacaggg gttccctatt tcaaggtgac
     3601 ctatgtttct attgaaaagg cctgattaga aaggccagtc atcagaccat gagtaattct
     3661 ccagcgacaa aaaagttttt cgacgccgtg ctcttccgcg aaatctggga tgcgatgaag
     3721 gtgacgctta agcatttgct gcatcggccc attacgtttc aatatccacg ggagaaacga
     3781 acgattcctg atgcccatcg tggcgcactt tgtctgctac ggtatgagga tgggaaagag
     3841 cgctgtgtgg gctgtgattt atgtgaggca gcttgtccat cccgctgcat taaagtcctt
     3901 agcgaagagg ataagactct cccgctccaa cgctatgcca ctgaatttta cattgatatt
     3961 accaaatgcg tgttttgtgg atactgcgtc gaagcctgtc cggtcaatgc gctggcaatg
     4021 acgaaggttt atgaattttc aactcatgat aagcggaccc tcctctttga caaggcgcgg
     4081 ctctaccaga ttggagagcg tcatatagat gatgccaaaa agtatctcta tgcccataac
     4141 caagagaagg aaggggagga aggccgcgcc taccgctacc atttccctgt ttcaataaaa
     4201 agtacccagg aaccgtagtt tcatcatgat gtggcttctt ttcggatatt ttgccacggt
     4261 gagtgtcgtg gcaggggttt tgactgtggc attgcgaaat gccgtgcact gcgcgttggc
     4321 cttgctgacg cttctgctcc atgtcgccgg tctgtttgtc ctattgaatg cggaatttct
     4381 ttttgcggtg caaatcattg tgtatgcggg agcgattctt attttgtatc ttttcgtgtt
     4441 gatgttgatg aatttaaaaa cggaggaaca gcatctccat aaaaaatatg cctttttagt
     4501 gtttgccgga accgcaattt ttggagaact cctgattctc ctccttcagt caccttatgg
     4561 cggtgtgatt ggagcggcca cacctgacct catcctgcaa acggggccca gccatgccat
     4621 tgggatcacg at
//
LOCUS       DATIAL010000221         3752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_899027, whole genome shotgun sequence.
ACCESSION   DATIAL010000221 DATIAL010000000
VERSION     DATIAL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899027"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VLA60_06940"
     CDS             <1..732
                     /locus_tag="VLA60_06940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HSF09131.1"
                     /translation="ASFAAFLRVLLEAFGGIKPDWNLLILVICIITVALGNLVAIVQT
                     NVKRMLAYSSIAHAGYALIGVVVAGWAGIEGAVSSQGVSSLMLYLAIYSFMTIGAFSM
                     VAILRKGGLEGEEIEDFTGLAKRHKGGAFLMLVFMVSLAGIPPTAGFIGKLYLFMAAV
                     NAGLAWLAVIGLIFAAISAFFYLRIVMVMYMREPSSEQELHTRLVLSPQVSFVLACAV
                     AGVVLLGIFPGPLVSVADLSSLSLK"
     gene            complement(788..1666)
                     /locus_tag="VLA60_06945"
     CDS             complement(788..1666)
                     /locus_tag="VLA60_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HSF09132.1"
                     /translation="MSWMYFALLAALSESLKDLLSKQGLRSVSPQLAALAAGATPIPI
                     LLSIVLFTDSVPLLGPRYGWALLIGGTLNILAMFQFMRALQSSDLSLAIPFVSFTPLF
                     LLITSPFLVGDIPRTQDIVGIFCIVGGAYVLHIQSIHHGLLAPLFAIFREPGPRRMLS
                     VAFIYSLTSNFDKIGVQNSSPIFWSLSISSVMTIGFVFLLRFLPAKNMAAPHLTTLGI
                     LLLVGLFQTIGLLVHNTALSLGPVPSVIAIKRSSILFAVMWGYIFLKEKYVGERLIGA
                     ALMMIGVVVLGFGQTD"
     gene            complement(1864..2649)
                     /locus_tag="VLA60_06950"
     CDS             complement(1864..2649)
                     /locus_tag="VLA60_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSF09133.1"
                     /translation="MPQSREFTIVKYVAVCIGILLSYALASEWNTFSFSQEPDLIQAI
                     QNRNLPQVQKLVIQGSDVNQRTPQGATPLHFSARSGQFTITRLLLQKGADPEAIYQSE
                     WTPLHFAAKGGHVDIADLLLQHGAPVDGFKGNIHPLHIAVQEHHHRMASFLLAHGATV
                     KSSFKEGWTALHIAAQTGDADMTRLLLDSGAPIDATNTIGLTPLHSAALSGQRNLTHY
                     LLSRGATCSLPSQPLQSSTSATFGQLYAALQEVLQHCPNPLAS"
     gene            complement(3012..3668)
                     /locus_tag="VLA60_06955"
     CDS             complement(3012..3668)
                     /locus_tag="VLA60_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09134.1"
                     /translation="MDFTLMFVVLLVCLAFGAIMLTVSMGPPTKNKKEESRIQGISFI
                     PSQMFMGTDGVGGIAVNDQTLKICLLHSPAVPPQLIHISSLVGSFLIKNGEVLNETLR
                     TAPKVLISFQKLMHANAERLIKSWQTSPSQKDNQRIDLIVWIHDEDEPFHVVNFLNME
                     TKEGGILFEKSLGTATHWHKVLDGLILQADQLACIQMESDQDDTNQPTKNPALTSAAS
                     "
BASE COUNT          913 a          805 c         1076 g          958 t
ORIGIN      
        1 gccagctttg ccgcgttcct tcgtgttctt ctggaagcct tcggtggaat caaaccggat
       61 tggaatctgt tgatcctggt tatatgtatt atcacggtgg cccttggcaa ccttgtggcc
      121 attgttcaga ccaacgtgaa acggatgctg gcctattcca gtattgccca tgcgggttat
      181 gcattgattg gtgtcgtggt cgccgggtgg gccggcatag agggagcggt ttcgtctcag
      241 ggcgtttcga gcctgatgct ctatctggcc atttattcgt ttatgacgat aggggcattt
      301 tccatggtgg ccattttgcg gaagggtggc ctggaaggcg aggaaataga agatttcacc
      361 ggattggcca agcgacacaa gggtggcgcg tttctcatgt tggtgtttat ggtgtcgttg
      421 gcgggtattc ctcccaccgc cgggttcatt gggaagctgt atcttttcat ggcggcggtt
      481 aatgccgggt tggcatggct ggcagtcatt ggattgatct ttgccgccat ttcagctttt
      541 ttctatttgc gcattgtcat ggtgatgtat atgcgtgagc catcttccga acaggaattg
      601 catacccgat tggtcctttc tccccaggta tcgtttgtgc tggcctgcgc agtggccggt
      661 gtggttttgc tcgggatctt tccaggtccg ctggtttcgg ttgcagacct ttcctctctc
      721 tccctgaaat aactctcggt tgtgtgattc aactgctgtc tttcggtttg ttgtcgggct
      781 gagaagatta gtccgtttgc ccgaaaccta atacgacaac tccgatcatc attaaggctg
      841 cgccaatcaa ccgctcccct acgtattttt ccttcaaaaa tatatatccc cacatgacgg
      901 cgaagagaat actcgatcgc tttatggcga tgacggaagg aacaggtccc agactgagag
      961 cggtattgtg aacgaggagc cctatggtct gaaacaggcc gaccagcaaa aggatgccga
     1021 gagtggtcag gtgaggagcg gccatattct ttgccggcag gaatcgtagc aagaatacaa
     1081 acccgatggt catcacgctg gaaatggaca ggctccaaaa gatgggagag gaattctgca
     1141 cgccgatttt gtcgaaattc gacgtgaggc tatatatgaa cgccacggaa agcattcgcc
     1201 tgggacccgg ttctcgaaag atcgcgaaca atggcgcaag tagaccatgg tgtattgact
     1261 gaatgtggag gacataggct ccccccacaa tacagaagat gcctacgata tcctgtgtcc
     1321 ttgggatatc cccgaccagg aaaggtgagg tgatcaataa aaaaagagga gtaaatgaaa
     1381 cgaagggaat ggccagggaa agatcggaac tttgtaaggc tctcatgaat tggaacatgg
     1441 ccaggatatt cagggttcct ccaatgagca gtgcccatcc ataccggggg cccagcaaag
     1501 gaactgaatc cgtgaacagc acaatgctca gaagtatggg aatgggagtc gcaccagcgg
     1561 ctaaagccgc cagttgaggt gagacggatc gcagtccctg cttacttaat aggtctttca
     1621 gggattcgct gagtgccgcc aacagagcaa aatacatcca gctcatgcag ggatcctcaa
     1681 atcagacgat ttcgaggtga aataattgat cacaaatttt gtctgaatgc acgttgctag
     1741 gaacgtatgt ccaggatctt acaatgaata tctggatgcc accagtggtt tcatctgatg
     1801 ttaccgggaa gatgcggaga ctaataggaa aggggccgga gtcagggggc ttacggcgaa
     1861 gacttaggaa gccagagggt taggacagtg ctgtaagact tcctgtagag ccgcatacag
     1921 ttggccgaat gtggcagacg tggagctttg gagcggttgt gagggaagag aacaggtggc
     1981 tccacgtgac agcagatagt gggtcagatt ccgttggccg gagagagcgg cggaatggag
     2041 gggcgtgagg ccgatggtat tggtggcatc gatcggcgcg ccggaatcca gaagtaggcg
     2101 agtcatgtcg gcatctccgg tttgtgcggc gatgtgcagc gcagtccagc cttccttaaa
     2161 tgaggatttg accgttgcgc cgtgagccaa cagaaaagac gccatccggt ggtgatgctc
     2221 ttgcacggca atgtggagtg ggtggatgtt tcctttaaag ccatccactg gcgcaccgtg
     2281 ttgaaggagg aggtcggcga tatccacatg tccccccttc gcggcaaaat ggagaggcgt
     2341 ccattcggac tgataaatcg cctcagggtc cgctcctttt tgtaacagaa gacgagtaat
     2401 cgtaaattgt ccggatcttg ccgaaaaatg gagaggcgtc gctccttgtg gggtgcgctg
     2461 attgacatcg gatccttgaa tcaccagctt ctgaacctgg ggcaaattgc gattttggat
     2521 ggcttgaata agatcaggtt cttgggagaa ggagaaggtg ttccactctg acgccaatgc
     2581 ataggacagc agaatgccaa tacataccgc cacatatttg actatagtga attcacgtga
     2641 ttgcggcatg agagaaagat ttccttgttt gaaagatgaa tgcaaacggc atcaggagtg
     2701 ggcataatga gtatcaagat gtagcgaacg tcaaagcggt ggtctgcacc attccccatt
     2761 tgaaggggag cgtcctcgcg ctctttttgg tgtgcctgtg gattgcgtgg ggaatctatg
     2821 ctggaaaaat tacaagtgac ttttggaaat cctgaaagaa ccgagtaggt caggcccggt
     2881 attcacaaat gggtgggggt acggacaacc ctgtaagttc ttgacgaacg caacaaggag
     2941 gattttatgg aagggggcag aaaggaacta tggggtctgg ggatacggcg ggtgagacag
     3001 gagcgcggaa tctatgaagc tgcgcttgtt aaagcagggt tctttgtcgg ctgattggtg
     3061 tcatcttggt ccgattccat ctgaatacac gcgagttggt cggcttggag tatcaatcca
     3121 tccaaaacct tatgccaatg tgttgccgta cctaaggatt tttcaaagag aatgccccct
     3181 tccttggttt ccatattgag gaaattgaca acatggaagg gttcgtcttc atcgtggatc
     3241 cacacaatga ggtcgattcg ctggttgtct ttttgggaag gggaggtctg ccaacttttg
     3301 atcaatcttt ccgcgtttgc gtgcatgagt ttttgaaatg agatgagtac ttttggggca
     3361 gttcgtaacg tttcgttaag gacctctcca ttttttatta ggaacgaacc gaccaggctt
     3421 gaaatatgaa tcaattgtgg tgggaccgca ggactatgta acaggcaaat tttgagcgtt
     3481 tggtcgttga cagcaatgcc tccaacgcca tcggtaccca tgaacatttg tgatggaatg
     3541 aaacttatgc cctggatccg gctttcttct tttttattct ttgttggagg acccatgctt
     3601 actgtgagca taatggcacc gaatgcaaga cataccaaca acacaacgaa catcagggtg
     3661 aaatccatcg atgaatcctt atgactatga aagtccttac agtgttgtta acagatcgaa
     3721 acgggatatg actcgtgagt atggtgaagt tt
//
LOCUS       DATIAL010000222         2991 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_255776, whole genome shotgun sequence.
ACCESSION   DATIAL010000222 DATIAL010000000
VERSION     DATIAL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255776"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VLA60_06960"
     CDS             <1..1774
                     /locus_tag="VLA60_06960"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HSF09135.1"
                     /translation="VSANQSLSKAFNVALHSGSVMGFALVSLAALGLLVIYLGLLSMM
                     PAQLPTHILMEVIAGFGLGGSSIALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPR
                     NPAVIADNVGDNVGDVAGMGADLFGSCAESTCAAMVISAVAFSGMVDALLYPILISAI
                     GIPVSFLTMMMIRVNSEEQVGPALKKMLIISSAMMAVVMLFVTKFMLPDTFQIGGETY
                     SDLGVYFCFLAGLIAGLAVGLMTEYYTSHDYAPVREVAKSCETGAATNIIFGLALGYK
                     SAVGPYLAIGLAIYIPWMMAGMYGVAIASLGMLGTLVIALTIDAYGPVSDNAGGIAEM
                     AGMPEHVRKRTDVLDAAGNTTAAIGKGFAIGAAILTSLALFAAFLTRADLLLKEQHGP
                     EFDLLGSINLLDPLVYTGLFIGAVLPAYFSAMTMKSVGSAAYKMIEEVRRQFRTIPGL
                     MEGKAEADYELCVAISTQAALKEMIAPGLLIMGTPLVTGFLFGIQAVAGVLAGSLVAG
                     GVIAISSSNSGGAWDNAKKFIEAGNLGGKGTDVHKAAVVGDTVGDPLKDTSGPSLNIL
                     IKLSAILSLVFVPFFVQYGGILFR"
     gene            2242..>2991
                     /locus_tag="VLA60_06965"
     CDS             2242..>2991
                     /locus_tag="VLA60_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSF09136.1"
                     /translation="MEPPNRPDRLSIHKTGEPESVSPTDMETLMHVISHDLRAPIVTI
                     QGFCQELMMACNRLNELFEEEVLSDRVREHLNPLVTQDIPEAVRFIRAGADNVNSVTS
                     GILRFIRLGQMAIQWEGLDVNRLVMGIVTSMEFQLKKKEVVLHIGDLPDCMGDDVLVT
                     QVFANLIDNAQKYLDPARPGEIVISGKTINGWSVYSVSDNGIGIPVEHHAHIFQVFHR
                     LDTQQESGQGLGLAIVRRIIDRHQGKIEVEST"
BASE COUNT          622 a          664 c          849 g          856 t
ORIGIN      
        1 ggtctcagcc aatcagtccc tctccaaagc ctttaatgtg gcgctgcatt cgggttccgt
       61 aatgggcttt gccttagtca gtttagcggc cttaggactg ctcgtgatct acctggggtt
      121 attgagtatg atgccggcac aacttccaac gcatattttg atggaagtca ttgccggctt
      181 cggtctcggg ggctcgtcca ttgcgttatt tgcccgtgtg ggtggcggta ttttcaccaa
      241 agctgcggat gtcggggcgg atttggtggg aaaagttgaa gcgggtatcc ccgaagatga
      301 tccgcgtaat cctgcggtga ttgcggataa cgtgggcgat aatgtcggtg atgtcgcggg
      361 aatgggagcg gacctgtttg ggtcctgtgc cgaaagcacc tgcgccgcca tggtgattag
      421 cgccgtcgca ttctccggca tggttgatgc cttattgtat cccatcctca tctcggctat
      481 cggaattccg gttagctttc tcaccatgat gatgattagg gtcaattccg aagaacaagt
      541 tggtccagct ttgaaaaaga tgctgatcat ctccagcgcc atgatggccg ttgtgatgtt
      601 gtttgtcacc aagttcatgc ttcctgatac ctttcaaatc ggaggggaga catattcgga
      661 tctgggagtg tatttttgct tccttgcagg gttgattgcg gggcttgccg tgggtttgat
      721 gacggagtac tacacttccc atgactatgc tcctgtgcgg gaagtggcga aatcgtgtga
      781 aacaggtgca gccacgaata tcatttttgg ccttgccctt ggctataaga gtgctgtggg
      841 tccctatctc gccattggtc tcgccatcta tattccgtgg atgatggccg gtatgtatgg
      901 ggtggcaatt gcctccctgg gtatgttggg gacacttgtg attgccctga cgattgatgc
      961 ttatggaccg gtttcagata acgccggtgg cattgcggaa atggccggta tgcctgaaca
     1021 cgtacgaaaa cggacggatg tgctggacgc ggctggaaat accacggcgg ccattgggaa
     1081 gggctttgcc atcggtgcgg ccatcctgac gtcgttggcg ctgttcgcgg cgttcctcac
     1141 cagggcggat ttattgctca aggaacaaca cggcccggaa tttgatttgt taggcagtat
     1201 taatttgctc gatcccttgg tgtataccgg gttatttatc ggcgcggtgt tacccgctta
     1261 tttttccgcc atgacgatga agtccgtcgg gtctgcagcc tataagatga ttgaagaagt
     1321 tcgccgacag ttccgcacca ttcccggtct gatggaaggg aaagccgaag cggattacga
     1381 gctctgtgtc gccatttcca cgcaagctgc cctgaaagaa atgattgcac cgggccttct
     1441 gatcatgggc acaccgctgg ttacggggtt cctgtttggt attcaagcag ttgcgggtgt
     1501 tctcgccggg tctctcgtgg ccgggggcgt gatcgcgatt tcctcttcca atagtggtgg
     1561 tgcctgggat aatgcgaaga aatttattga ggccggaaat cttgggggca agggaacgga
     1621 cgttcacaaa gcagcggtag tcggtgatac cgtcggagat cctcttaaag atacttcagg
     1681 gccctcgttg aatattctga tcaaactttc ggccattctg tcactggtgt tcgtgccatt
     1741 tttcgttcag tacggcggaa ttttgttccg gtaattttcg ggtgctccat gtgtcgtgtg
     1801 ttggaatggt gacaaggtta ccggtgatag acggaggttg attaattatg tatcctgcga
     1861 tgaacgtgat taatatgtgg atgctcttat ttattctgct ccttgtgtaa tttttatctt
     1921 cattccgttt ttatcctctt tgcctggaat gaccacaatt tcatgttatc ggtcatcctc
     1981 ggattatctc accagcatta ccggaaagat tgatgttctg acgtttccta tgggttgata
     2041 gccagtctgg taaatgagga gcagtaatga ggcgctcccg ttttgacatt catggttatg
     2101 cccgccctgt gagattggtt agcggcacta caatgggatg ctgccgtctt ccttactgac
     2161 tttggtttca cctgactctg gcaattggaa aagaaattcc ataattaatt gaggtgaccg
     2221 gttgaaaagg aatgctgtgt gatggaacca cccaatcgac cggataggct ttctatccat
     2281 aagacgggcg agcctgagtc tgtttccccc acggatatgg aaacgctgat gcatgtgatc
     2341 tctcatgatc ttcgtgcccc gattgtgacc atccaggggt tttgtcagga acttatgatg
     2401 gcctgtaatc gcctgaatga attattcgaa gaggaggtac tgtcggacag ggttcgggag
     2461 catcttaatc cgctggttac ccaagatatt cccgaagcgg tacggtttat tcgagccggg
     2521 gcggataatg tcaattccgt cacctccgga attctccggt ttatccggct gggacagatg
     2581 gccattcagt gggaagggct ggatgtgaat cgacttgtga tgggtattgt gacctccatg
     2641 gagtttcaac ttaagaaaaa ggaagttgtg ttgcacatcg gggatcttcc cgattgtatg
     2701 ggtgatgatg tgctggtgac ccaggttttt gcgaatctga tcgataatgc ccagaaatat
     2761 ctcgatcctg ctcgtccggg agagattgtc atttcaggca agacaatcaa tggatggtcg
     2821 gtctattccg tttctgataa cggaatcggg atccccgtgg aacatcatgc tcatattttt
     2881 caggtatttc accgtctgga cacgcaacag gaaagcgggc aaggtttggg cttggccatt
     2941 gtgcgacgga tcattgatcg gcaccagggt aaaattgaag tcgaatcgac t
//
LOCUS       DATIAL010000223         3516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1007143, whole genome shotgun sequence.
ACCESSION   DATIAL010000223 DATIAL010000000
VERSION     DATIAL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007143"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VLA60_06970"
     CDS             complement(<1..235)
                     /locus_tag="VLA60_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HSF09137.1"
                     /translation="MADITIYHKPSCSTCRQVIKLVQESGQLFTAINYYEKPFSKSTL
                     KGLLKKAGLKSSDIIRTKEELYKKLNLHQSQFTD"
     gene            374..736
                     /locus_tag="VLA60_06975"
     CDS             374..736
                     /locus_tag="VLA60_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09138.1"
                     /translation="MSEPTPPQEFFCASETERTLKDLRIKRKGQPVYVMGHEDRYKGK
                     EGVFEFFNVRLAVVKFPDEKTLGFDPMDLLLPCEINADGVPFFEIRYCETCDQVFPLT
                     AAEFYAAVERKECPECAP"
     gene            935..2407
                     /locus_tag="VLA60_06980"
     CDS             935..2407
                     /locus_tag="VLA60_06980"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HSF09139.1"
                     /translation="MTRRPVSRKSTSYRGNTPSLSAASRSGRTRIERDTMGPVQVPVS
                     AYYGVQTARAIENFPISGIRFPRPFIRALGLIKWAAARTNESLGILEKKPARVIQQAA
                     REVVAGQWDEHFPLDIFQTGSGTSTNMNANEVIANRASEILNGDRASRKVHPNDQVNL
                     GQSSNDVIPTAIHVAAIEDIHKMLLPCLRRLHKALQQKARQFNRTVKIGRTHLQDATP
                     IRLGQEFGGYARQIELAIQRAKHAQAVLAELALGGTAVGTGLNTHPKFASMVIRLLSK
                     EIGYTMREAENHFEAQSTQDSVVEASGCLRTIAVSLMKIANDLRWMGSGPRCGLGEIQ
                     LPETQPGSSIMPGKVNPVIAESVTMVATQVIGNDMAITVAGQAGNFELLVMLPVMAHN
                     LLQSINLLAKSSENFSSRCVEGLVANKSRCEAYIERSLAMCTGLAPVIGYESAAAIAK
                     ESYKTGQTVREVAKSQDVLPEAEITKLLDPWRMTGLTCSP"
     gene            complement(2486..3340)
                     /locus_tag="VLA60_06985"
     CDS             complement(2486..3340)
                     /locus_tag="VLA60_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HSF09140.1"
                     /translation="MAIFQTIGIITKPNAPNIQQAVNHICDWLTAHHKTVLIDSLQGI
                     PPPDSATRLQIAKEADLLLVLGGDGTMLSGARLVEQREIPILGVNMGGLGFLTEINLE
                     DLPKALEKVFAGNYQLDQRLMLKIQLQRKDQILGTYSALNDVVVSKGHLARMIATKIW
                     VNHAFMTNLRGDGLVVATPTGSTAYSLSAKGPILDPRLEVLLINPICPHTFSHRPFLA
                     PGDVPITIELTSEEPAMATIDGQVGAEMLTGDLVHVRASDHRTQLIRFPDRSFFEVLR
                     TKLKWGDG"
BASE COUNT          875 a          786 c          921 g          934 t
ORIGIN      
        1 catcggtgaa ttgtgattgg tgcaagttca attttttgta taactcctcc ttggtgcgga
       61 tgatgtcgga agatttaaga cctgcctttt taagtaatcc ttttaacgtg gatttcgaaa
      121 atggtttttc atagtaattg atcgcggtaa aaagttgacc gctttcctgg actaatttga
      181 tgacttgtct gcaggtggag caggatggtt tatgataaat cgtgatgtca gccatgaacg
      241 cgcctccttt gtttgaaaat ggcccggacc ccttccgcct tgaccttttt tttgaggcca
      301 tgctacaagc tgttccattc ttatgcggaa ggatgaatgt gtagactatg cctgaacaag
      361 gacatttacc gggatgagcg aacctacgcc cccccaggaa tttttttgtg cctccgaaac
      421 ggagcgaacc ttaaaggatc ttcgcatcaa acgtaaaggg cagcctgtgt atgtcatggg
      481 tcatgaagac cggtacaagg ggaaggaagg tgtgtttgaa ttttttaatg tccggcttgc
      541 ggtggtaaaa tttcccgatg aaaaaacctt gggatttgat cccatggatt tattgctccc
      601 ttgtgagatc aacgcagatg gggtgccttt ttttgaaatt cgttattgcg aaacatgcga
      661 tcaagtgttt cccttaacgg cggccgagtt ttatgctgcg gtggaacgga aggaatgtcc
      721 cgagtgcgca ccgtaatgct caaagagaaa cagtttcttg cgctgtttgg ttcattctgt
      781 ctttcctctt ctttcccgtc atttctagaa tccttgtgat tcccaggatt gacttctcca
      841 aagtgttctt ctgtacctgt cctgtacaag agatttgaat gtgcgatttc tgaatgtgta
      901 gcgccgcatt taagccatag aaaggatccg gctcatgacg cgtcgcccgg tttcccgaaa
      961 atcaacatca tatcgaggca atacgccatc tctgtcggcg gcatcccgct ccggtcgaac
     1021 ccgtatcgaa cgggatacga tggggcctgt acaagtgccg gtgtcagcct attacggggt
     1081 acaaacggcg cgagccatag aaaactttcc gattagcgga attcgatttc cgcgtccctt
     1141 tattcgtgca ctgggattaa ttaaatgggc agctgcccgg acaaatgaat cgttgggtat
     1201 tttagagaaa aaaccggctc gtgtgattca acaagcggcg cgagaagtgg ttgccggaca
     1261 atgggatgaa cattttcccc tggatatttt tcaaacaggg tccgggacct cgacgaatat
     1321 gaacgccaac gaagtcatcg cgaatcgcgc gtcggaaatc ctgaacgggg atcgagcaag
     1381 ccggaaggtg catcccaatg atcaggtgaa tctgggacaa tccagtaatg atgtgattcc
     1441 cacggccatc catgtcgcgg ctattgaaga tattcacaaa atgttattgc catgtctaag
     1501 acggctgcac aaggctcttc aacaaaaagc aaggcaattt aaccgcaccg tgaagatcgg
     1561 acgaacacat ttacaggatg cgacccccat tcggttgggg caggaattcg gaggctatgc
     1621 gcggcagata gaactagcca ttcagcgagc caaacatgcc caagccgtgc tggcggaatt
     1681 agccttgggc ggaaccgctg tgggaacggg cctcaatact catccaaaat ttgcttccat
     1741 ggttatccgg ctattatcaa aagaaatcgg ctataccatg cgggaggctg aaaaccattt
     1801 cgaggctcag tccactcagg attcggtagt agaagccagc ggatgtttac ggaccattgc
     1861 cgtgagctta atgaaaattg ctaatgatct ccggtggatg ggatcgggtc ctcgatgtgg
     1921 cctgggcgaa attcaacttc cggaaaccca acccgggtcc tctattatgc cgggtaaggt
     1981 gaacccggtc attgctgaat ccgtcacgat ggtagccact caagttattg gcaatgatat
     2041 ggcaattacg gttgccgggc aggccggaaa tttcgagtta ttagtcatgt tgcccgtaat
     2101 ggctcataat ctcttgcagt cgattaacct gcttgcgaaa tcctcagaaa atttctccag
     2161 ccggtgcgta gaaggtcttg tggccaacaa aagcaggtgc gaagcataca ttgagagaag
     2221 tttggccatg tgcactgggt tggctcctgt tattggatac gagtcagcgg cggctattgc
     2281 caaagaatcc tacaaaaccg gtcaaaccgt gcgtgaggtt gcgaaaagtc aggatgtttt
     2341 gccggaagcg gagatcacga aacttttaga tccctggcgc atgacagggc tcacttgttc
     2401 tccataagag ggctcgttag cttgttccat taatgggtta ttgaccggaa ccggtccagt
     2461 caggataggt cccgcaagag cgcacctacc catcgcccca tttcaatttt gtgcgtaaca
     2521 cttcaaaaaa gcttcggtct ggaaaacgta tgagttgcgt gcgatggtcg gaagctcgaa
     2581 cgtgaacaag atcacctgtc agcatctcgg ctcccacttg gccatcgatt gtggccatgg
     2641 ccggttcttc actggttagt tcaatggtga tgggtacgtc gccgggggcg aggaatgggc
     2701 ggtgcgaaaa agtgtgcgga cagattggat taatgagcag aacttccaat cgtggatcta
     2761 aaatcggtcc ctttgccgaa agggagtaag ctgttgatcc tgtcggagta gcaacgacca
     2821 gtccatcgcc acgcaaattt gtcataaagg catgattcac ccatatcttt gttgcgatca
     2881 ttcttgctaa atgtcctttg ctgaccacca cgtcgtttaa ggcagaatag gtgccgagga
     2941 tttggtcctt tcgctgaagt tggattttta gcatcaagcg ttgatccagc tgataatttc
     3001 ctgcgaatac tttttccaag gccttgggga gatcctcaag attaatttca gtgaggaacc
     3061 ccaggccacc catgttgacc cccaaaatgg gaatctccct ttgttccaca aggcgcgctc
     3121 cgcttaacat cgtgccgtcg cctcctaaca caagaaggag atcggcttcc ttggcaattt
     3181 gtaaacgagt ggcgctatca ggaggaggaa ttccttggag agaatcaatg aggacagttt
     3241 tgtgatgggc cgtcaaccag tcacaaatgt ggttgactgc ctgttgaata ttaggggcgt
     3301 ttggtttagt gatgattccg attgtctgaa agatggccat gagttatgaa gggggtggta
     3361 gtgccaggag acacgatgtt agtgtgttag aaagttgata gcaagagaag gttcttttga
     3421 cgcgggtgaa gaaggctttt cttaaattaa attttgtatt aaagtaagac agaatggctt
     3481 tgcctcatct tctcttgtcc aggcctttga cttgac
//
LOCUS       DATIAL010000224        10978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1114993, whole genome shotgun sequence.
ACCESSION   DATIAL010000224 DATIAL010000000
VERSION     DATIAL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10978
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114993"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VLA60_06990"
     CDS             <1..918
                     /locus_tag="VLA60_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF09141.1"
                     /translation="TVKVERAGDVIPDVVERVPMAGEVRGAPFQPPTTCPVCQSHTIQ
                     EGPILYCTGQTVCSAQLKGSLEHFASKGALNIEGLGKKTVAQLVDKGFVKDLSDLYTL
                     GVDDLLQLEGFADKSARQLLGEIERSKEVPFARLLIGLGIRHVGTHIARVLAQNFGSL
                     DNLKKATEGELLQIRDIGPEIAASVTHFFKESRNLKVWQRMESLGVRVQQEASLSEPH
                     AQPLKGKIFVLTGTLNGYSRQEAKRRIEELGGRVTSSVSKQTDYLIAGEDPGSKYDKA
                     TTLGVRILDENEFSLFIQPGNILSSSSNE"
     gene            complement(1074..2345)
                     /locus_tag="VLA60_06995"
     CDS             complement(1074..2345)
                     /locus_tag="VLA60_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HSF09142.1"
                     /translation="MDFIAVLICLGLSAFFSGAEIAFFSITESRLKTLSDEGHKGAKL
                     ALKLRSNPQRLLSTILIGNNLVNIMAASMATLIAIRLFGSEAVAVATGLLTFMVLLFG
                     EIVPKTLCAKYSETAVQLLAYPIYLLEQLFFPFLYFLEPFILKLTGGRGLTVPFITEE
                     ELKIMLDAGGKAGVLETDEVKMIKNVFDFNDVTAEDAMTPRIYMFALDGNQTLGEARE
                     ELFKAKFSRIPIYEGNPDNITAILYRNHALMELAQSHTNLTLKSFAKPALFIPSTKTA
                     DDLLRQFQQEKRHIAIVVNEFGGVMGLITVEDLLEEVVGEILDEGDLSEEWIRRTGKN
                     QILVDGRTEVRRINEFLKVELDEEQNTISGLILEELGHIPAVGEKVQTDNCLLIVQEA
                     DERSIKGVQIIKEEPVPETPPTEPSTVSSES"
     gene            complement(2596..3264)
                     /locus_tag="VLA60_07000"
     CDS             complement(2596..3264)
                     /locus_tag="VLA60_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HSF09143.1"
                     /translation="MNSGIPVKRICLILTFLLMAGCAGGQSRTEPMISDTFQRGVASW
                     YGPSFHGNPTANGEPYDMWALTAAHPTLPFGTRVLVQSLDTGKSVTVRINDRGPFIRG
                     RIIDLSYGAARELAMIGRGTENVSLTILESRNTSSGNMSKGGSDSYWVQVGSFTTLTQ
                     AVTLHEQLAYHYPNLRLTTVDLPSGQWHRVQIGTFPSQEKAKIVAKELEKQFHLDYLL
                     IQGN"
     gene            complement(3368..4603)
                     /locus_tag="VLA60_07005"
     CDS             complement(3368..4603)
                     /locus_tag="VLA60_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09144.1"
                     /translation="MTQEDARAYLNYLLTLHLRQEEAFGPLGLAFVKENDLNKLSLLP
                     EEQFNLLMAIATVFSAEPKRYTMKLELLQKAYQLLPQTRYHDPELGRDLEHLIKKTQS
                     DLHRYNEAMKVARSQSPDRQSLIVETDIPEYFLETAQKRASAYYQEKYRLTKEAKTAQ
                     HFGGAAKKFEPDNLAIHKEFPGACAPFINARTNAFHIVLPFDLKISRSPEDPLEAGIR
                     IFYGKMGYSFPLRYEMGKLCSYHDGQVLDVDLRDPNLIFFSVSGIKDPEFITQASQTD
                     PSLPPELVYPMAVLEHTGSLGPFIQVSCNIKVWFDASMVSLLIQGAPDLPDYGLQGGA
                     GLMTRTYASDKVESYVHNLSQPWQEGLSFNFINLHLQLSRGIDSAVVPSGTPIFSVYP
                     VFNRQSYRFVDRRTMDQAS"
     gene            4773..5195
                     /locus_tag="VLA60_07010"
     CDS             4773..5195
                     /locus_tag="VLA60_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09145.1"
                     /translation="MHQAFFLKIRGAASMLGIGWIMACLFLGSGATFVSAEDTPGKKT
                     GSVAARGDDLSTESLNKQITDLEIQIQKLRDQSIELQEKTRAKLQAQLDNFKKQQDTL
                     IPRIEQLRDNSETAWQDIKENIQKAIEDLKLSVDSMKK"
     gene            5387..7798
                     /locus_tag="VLA60_07015"
     CDS             5387..7798
                     /locus_tag="VLA60_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HSF09146.1"
                     /translation="MVQHTEQVLEWPVLIDCLANEAASTMGAARCRALVFAADLQNAR
                     IQQQETTEMVDILEGAYPLPSMVFPDIRHVLARAEKEGVLDGPDLRDIASVVGLGHRI
                     RHHLEIHGSPFPMIRARCEKLQDLMWIKEVIDSCIDRNGHLRESASPELYQLTQKRQG
                     LRQAMRRQLEGMLASQEYEDLLQGQYFAERENRYVIPIKAERQHTIDGIVHDISGSGA
                     TVFIEPRHLIELNNSIKFADLQVAQEERHILQDLSSRVAEHVWALRENLSCLADVDCL
                     MAKARLSIKTQGSRIHLTQEHCIDLQQARHPLLALTKEHVIPNSIRIEGDTTVLIISG
                     PNAGGKTVSLKLVGLFAMMVKVGLHPTCGPDSKMALFGQVYADIGDSQDLSKDVSSFS
                     GHILNMIALLENIGSPTNPANRDVLVLLDEVGSSTDPIEGAALAEALLTRLCEFGCTI
                     IATTHYPTLKTLAFRNPHVRNASQEFDMVTLAPTYRLIDGIPGGSSALEIAERLGLDS
                     TIIQSAQLLIQRHDYDLDAIFRALQNTHTRLEREYEQAQHLRQEAQHLFDEAEVTRNQ
                     LQAQEREDRQRYRKQWQQEFSKAQRQLNQIINDLKADKTLSKVRSIQQTIGSVNQEIL
                     SRLPHNSLESSEPPHEGDLVEIDPLGTIGILQESLEGKKQVSIRVGTQTIKTAPSAVR
                     RATASSGNKSLSGPQQLSRKRSLSTISTETSHPSSHQAAGQYQQELCIRGFRLDDAME
                     MTLAALDHALVTQAKYLKVIHGQGSGALKTGIRNFCQSSPYIQSFRPGDPAEGGDGVT
                     VIELR"
     gene            complement(7790..8818)
                     /gene="queG"
                     /locus_tag="VLA60_07020"
     CDS             complement(7790..8818)
                     /gene="queG"
                     /locus_tag="VLA60_07020"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HSF09147.1"
                     /translation="MSPRSILANQIKEEARRLGFDAVGVARLPPPLPPIPLPAAQNTI
                     SQPEESITLRLWERLKKWLDLGFHATMEWIAKDPKRRSNPEEVLPGCQSLVVVGMNYY
                     TDHTPDESKGAGRIARYAWGKDYHGFMKDRLRQLENFLHSHVPDIQTRSYVDTGPIME
                     KPWAQEAGIGWIGKHTNIVSTEFGSWLLLGEILTTLPLDCDEPAMDLCGTCSLCIQAC
                     PTGAIVEPYLLDAERCISYLTIEYRGRADDMPAGLRKKIGNRIFGCDDCLDICPFNVN
                     AKVTTEAGFQPFPWTLHPQLPELANLTKDEFQSLTTGSPLRRPKHEGFIRNVQIGLDN
                     HAPPIPLS"
     gene            complement(8849..9385)
                     /locus_tag="VLA60_07025"
     CDS             complement(8849..9385)
                     /locus_tag="VLA60_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09148.1"
                     /translation="MWYKEVHLYKNFLLYSCSNPHPLLQIGLTMNLYSNTIKVIIGQT
                     KTSRLLMSGIVVFLLTEIGLLGLINKGEAADSRSRTEANCTIGMVKGEAKAECQVPIP
                     NGCAVAQFPGYPEPWVDISKGGGTSCEFDEKQTDWKTSIVGSCQDCTTEQCTGRFIVM
                     FNCANNVPPANPGIPGQK"
     gene            complement(9573..>10978)
                     /gene="groL"
                     /locus_tag="VLA60_07030"
     CDS             complement(9573..>10978)
                     /gene="groL"
                     /locus_tag="VLA60_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055846.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HSF09149.1"
                     /translation="TAGDGTTTATVLAQAIYREGVKNITAGANPMEIKRGIDKAVEVA
                     IEELKKFSKPCQTKKEISQIGSISANNDHTIGDLIAEAMDKVGKDGVITVEEAKSMST
                     SLDVVEGMQFDRGYISPYFVTNADRMEASLEDAFLLIVEKKISAMKDLLPILEQVAKM
                     GKPLVIIAEDVEGEALATLVVNKLRGTLNVAAVKAPGFGDRRKAMLEDIATLTGGQVI
                     SEEVGLKLESVKLTDLGRAKRVNIDKDNTTIVEGAGDPKRIEGRVKQIKTQVEETTSD
                     YDREKLQERLAKMVGGVAVINVGAATETEMKEKKARVEDALHATKAAVEEGIVPGGGV
                     ALLRCVPALDKMKLDHDQQVGVNIVKRSLEEPIRQISINAGAEGSIIVEKVRGETTNR
                     GYNAGTGEFVDMVEAGVIDPTKVARCALQNAASVAGLMLTTEVMVTEIPEAKSEGAGM
                     PGGHDHGMGGMGGMGGF"
BASE COUNT         2866 a         2578 c         2583 g         2951 t
ORIGIN      
        1 accgtaaaag tggaacgggc aggggacgtc attccagatg tggtggaacg agtgcccatg
       61 gcgggtgaag tgcgtggggc tccttttcag ccgccaacga cctgtccggt gtgtcagtcc
      121 cataccattc aggaagggcc cattctgtat tgtactggcc aaaccgtttg ttcagcccaa
      181 ctcaaaggat cgcttgaaca ttttgcgtca aaaggtgcct tgaatatcga agggcttgga
      241 aaaaaaacgg tagcacagtt ggtcgacaaa ggcttcgtca aggatttatc cgatctgtat
      301 acattagggg ttgacgacct tcttcaatta gaaggttttg cagacaaatc cgcgaggcaa
      361 cttttgggag aaatcgaaag gagtaaggag gtgcccttcg cgcgccttct cattggcctc
      421 ggcatacgtc atgtggggac tcatatcgcc agagttttgg cccaaaattt tggttcactc
      481 gacaatttga agaaggctac cgaaggggaa cttttacaaa tccgggatat cggaccagag
      541 attgctgcaa gcgtgacaca tttttttaaa gaatcccgta atttgaaggt ttggcaacgc
      601 atggagtcat tgggagttcg ggttcaacag gaagcaagtt tgtcagagcc gcatgctcaa
      661 ccgctaaagg ggaagatttt tgtattaacc ggaacgttga acggttattc ccgtcaggag
      721 gcgaaacgca gaattgaaga gttaggtgga cgtgtcactt ccagcgtcag taaacagaca
      781 gattacctca tagccggcga agaccccggc tctaaatatg acaaggccac cacattagga
      841 gtacgaatat tagatgaaaa tgaatttagc ttattcattc aacccgggaa cattctatct
      901 tcatcatcca acgagtaata ttcccatcga attatcatta atgaggtttt gtctcatcag
      961 gaacgagagt gggttgcaag aaaaggatag atcaatttct ttgctgggat tgagcagggg
     1021 agacgatgtt aaattctacc agcaggatac attatacaac cggaaagttt gaattaggat
     1081 tccgaagaca ccgtagacgg ttcggtggga ggcgtttcgg gaacaggttc ttccttgatg
     1141 atctggacac ctttgataga gcgttcatca gcttcttgga caattaaaag gcaattgtcc
     1201 gtttgaacct tctcaccgac agcaggaata tggcctaatt cttcaaggat aagtccgcta
     1261 atcgtatttt gctcttcgtc gagttcgacc ttaagaaatt cattgatgcg gcggacttcg
     1321 gttcgtccat ccactaaaat ttgatttttc cccgtccggc gaatccattc ttcactaaga
     1381 tcgccttcat ccagaatctc gcctaccact tcttccagca gatcttcgac ggtaattaaa
     1441 cccatcactc ccccaaactc attgaccaca atcgcaatat gtcgtttttc ttgttggaat
     1501 tgccgtaata aatcatcagc cgttttggta gaggggatga ataatgccgg tttcgcaaat
     1561 gatttgaggg tgaggtttgt atgactttgc gccagttcca tcaaggcatg attgcgatag
     1621 agaatggcgg tgatattgtc cggatttcct tcgtaaatgg gaattcgtga aaatttcgcc
     1681 ttaaataact cttcccgtgc ttctcctagt gtttgattgc catcgagggc gaacatatat
     1741 atcctcggcg tcatcgcatc ttctgccgtc acgtcattga aatcaaatac gtttttgatc
     1801 attttcactt catctgtttc caagaccccc gcttttcctc cggcatcgag catgatcttg
     1861 agttcttctt ccgtgataaa cggaacggtt aacccccgac cccctgtgag ttttaaaata
     1921 aacggttcca agaaatagag aaacggaaaa aataattgtt cgaggagata tatggggtat
     1981 gccagtaatt gcacggccgt ttcagaatat ttggcacaaa gcgttttggg gacgatttca
     2041 ccgaataaca ggaccataaa ggttaagaga cccgtagcga cggctacggc ctcagatcca
     2101 aacaggcgga tggcaatcaa ggtcgccatg gaggccgcca tgatattgac caaattattg
     2161 ccaatcagaa ttgtcgagag aaggcgttgt ggatttgacc gtaatttgag ggcaagtttt
     2221 gcccctttat gaccttcatc ggacagggtt ttcagccggc tttccgttat tgaaaaaaat
     2281 gcgatctcgg cgccggagaa aaaggcggaa agtcctagac aaataaggac ggcgataaaa
     2341 tccataaagg gcttaacgag ctccgacgtg acaaaaatat gccgttacag caagattagc
     2401 tgcaagatct ggcagtctgg tcttggagga agttggtatc agaagagttt gagagggatt
     2461 cataataagg ccaaaattct cattgtttat gagaggtttt gagccctcaa tgcttgattg
     2521 ggtccaaagt aacatagggt attggtttaa ggcaattatt cgggatgaat tatcagatta
     2581 gctcttttat ttcagtcagt taccttgtat cagaagatag tcaagatgga attgtttttc
     2641 cagttctttc gccacgattt tggccttttc ttgagaggga aatgtcccaa tctggacacg
     2701 gtgccattgt ccggatggaa ggtccacggt tgtcaatcgt aaattgggat agtggtaggc
     2761 cagttgttca tgcaaggtga ctgcttgggt tagggtcgtg aaagagccta cctgcaccca
     2821 ataactatca gaccctccct tcgacatatt gccggaactt gtgtttctcg attcgaggat
     2881 cgttaaactg acgttttccg ttcctcttcc aatcatggcc agctcccggg cagctccata
     2941 ggatagatca atgattcgac cccgaataaa aggaccacga tcattgatgc gaacggtcac
     3001 ggatttcccg gtatcgagac tttgaacgag gacccgtgtt ccaaagggta gcgtaggatg
     3061 tgcggcagtg agtgcccaca tgtcgtaggg ttcaccgttg gcagtcggat taccatgaaa
     3121 acttgggcca taccaggaag ccactccccg ttgaaacgta tccgagatca tcggctcggt
     3181 tcgggattgg ccacctgcgc atccagccat gagcaggaaa gtcaggataa gacaaatcct
     3241 tttgactgga atgccactat tcatgacatg tttaccccac cgaagggtga aggtggtggt
     3301 attttttcga gttggatgat ttgggaagcg catgagtccc atggactcac cagttagcca
     3361 tggattccta ggaagcctgg tccatcgttc ggcggtcgac aaagcggtag ctctgtcgat
     3421 tgaacaccgg atatactgaa aaaatcggtg ttccagaggg gacaactgca ctgtcaatgc
     3481 cacggcttaa ttgaagatgg agatttatga aattaaaact caagccttct tgccagggtt
     3541 gagataaatt gtgcacatat gattcaactt tatcggaagc gtacgttctg gtcatgaggc
     3601 ccgctccccc ttgcaatcca taatcaggta gatccggcgc accttgaatg aggagtgaaa
     3661 ccatagaggc atcgaaccac accttgatgt tacaactgac ctgaatgaat ggccccaaac
     3721 taccggtgtg ctctaagacg gccatgggat agaccaattc cggtgggaga gaagggtcgg
     3781 tttgagaggc ttgggtgata aactctggat ccttgatgcc cgatacagag aaaaagataa
     3841 gattcggatc gcggagatcc acatccaaca cttggccgtc atggtaactg cataatttcc
     3901 ccatctcata gcgtaaagga aatgaatatc ccatttttcc atagaaaatt cgaattccag
     3961 cttccaacgg atcttccggg cttcggctga tttttaagtc aaaaggtaaa acaatatgaa
     4021 aagcattggt ccgggcattg ataaatggag cgcaggcacc gggaaattcc ttatggatcg
     4081 ccagattgtc cggttcaaac tttttcgcgg ctccaccaaa atgctgggca gtttttgcct
     4141 ctttggttaa tcgatatttt tcttgataat aggctgaggc ccgcttttgt gcggtttcga
     4201 gaaaatattc agggatatcg gtttcaacaa ttaagctttg tcgatctgga gattgtgatc
     4261 tggcaacctt cattgcttca ttgtaacggt gaagatcact ctgagttttt ttgattaaat
     4321 gctcaaggtc ccgccctaat tcaggatcgt ggtagcgtgt ttgcggtaga agttggtagg
     4381 ctttttgcag caattctaat ttcatggtgt aacgtttggg ttcagcagag aagaccgtcg
     4441 ctatggccat caacaaatta aactgttcct caggaaggag ggataatttg ttcaggtcgt
     4501 tctccttgac aaacgccaaa cctaacggac caaaggcttc ttcctggcgt aaatggagag
     4561 taaggagata gttgagataa gcgcgagcgt cttcttgagt catatgaatc cttattttac
     4621 gggatggcat tctaaggtaa gttcgtcatt ggagcaaggc atcgtgtgaa tttccatgca
     4681 ttttttgtgg aatcctcgtt gctttgatga ccataggaat gctcaaattt gagcatgcaa
     4741 gaaaacaggg ttttgggagg aaaggattca atatgcacca agcctttttt ctaaaaatca
     4801 gaggcgccgc ctctatgctg ggaataggat ggattatggc ctgtctgttc ctgggaagcg
     4861 gagcaacatt tgtttcggca gaagatacac ctggcaagaa aacaggatcg gttgcagcaa
     4921 gaggagacga tttatccacg gagagtctga ataaacaaat tactgattta gaaatacaga
     4981 ttcagaaact ccgggatcaa agcatcgagt tacaagaaaa aacccgagcc aaacttcaag
     5041 cccaactcga taatttcaaa aagcaacaag ataccctcat tcctcgtatt gagcaacttc
     5101 gtgataatag tgaaactgct tggcaggaca tcaaggaaaa tattcaaaag gccattgaag
     5161 atctcaagct ttccgttgat tctatgaaaa aataacgttg cgccggcctc tcggatagtc
     5221 attacgtttg cattaggtta cttgccaggg accccttgct tttatatcct tccagtacaa
     5281 ttttcttatt cacccttgtt ttctgaaaat ctcaccgcta aatccaaggc ttgcatcgat
     5341 tggaacgtct ttttgaactg acgcgctaaa ataaaaggtg tctcgcatgg ttcaacacac
     5401 agaacaggtc ttggaatggc ctgttttaat cgattgtctt gccaatgaag ccgcctccac
     5461 gatgggggcc gctcgctgtc gtgcgctcgt cttcgcagct gaccttcaga atgcccgtat
     5521 tcaacaacaa gaaaccacag aaatggttga tatcctcgaa ggtgcttatc ctctgccttc
     5581 catggtgttt cccgatatca gacatgtgct ggcacgggct gaaaaagaag gagttctgga
     5641 cggtccggat cttcgagata ttgcttcggt tgtaggcctg ggtcatagga tcagacacca
     5701 cctggagatt catggctccc cctttccgat gatcagagct cgttgtgaaa aacttcaaga
     5761 tctgatgtgg atcaaggaag tgattgattc gtgcattgat cgcaacggcc atctgcgaga
     5821 atccgcgagt cctgagctct atcaattaac acagaaacgt caggggcttc gtcaggccat
     5881 gcgtcgacag ttggaaggga tgttggcttc tcaagaatat gaggatcttc ttcaaggaca
     5941 atactttgct gaacgtgaga atcgatacgt gattccgatt aaagccgaac gacaacacac
     6001 gattgatggg attgtccatg acatttccgg tagtggagcc acggtgttta ttgagccccg
     6061 tcatctaatc gaattgaata attccattaa atttgccgac ttgcaggtgg ctcaagaaga
     6121 acggcatata ctgcaagatt tgtccagtcg tgtcgcagaa catgtttggg ccctccgtga
     6181 aaatttgtcc tgcttggccg atgtcgattg cctcatggcc aaagcacgct taagcattaa
     6241 aacgcaaggc tctcgcattc accttaccca agaacattgt attgatctgc aacaagccag
     6301 gcatccactc ctggcgttga ccaaggaaca cgtcatcccc aattccattc gtattgaggg
     6361 ggataccaca gttctcatca tttctggacc aaatgcgggt gggaagacgg tgtcattgaa
     6421 gctggtggga ttattcgcga tgatggtgaa agttgggctt cacccgactt gtggccccga
     6481 ttctaagatg gcactttttg gacaggtgta tgcggatatc ggggattctc aagatctcag
     6541 taaagatgta tccagctttt cgggccatat tctcaatatg atcgccttat tggaaaatat
     6601 cggatcgcca acgaacccag ccaacaggga tgtccttgta cttttggacg aagtcgggag
     6661 ctcgacggat cctattgagg gcgccgcgct agcggaagcc ctcttaaccc gtctttgcga
     6721 attcggatgt acgattatag cgaccactca ttatcccacc ttgaaaaccc ttgcttttcg
     6781 aaatcctcat gttcggaatg ccagtcagga attcgatatg gttactcttg ctccaacata
     6841 ccggctcata gacggaattc ctgggggctc ttcagccttg gagattgccg aacgattagg
     6901 gttggattct acaatcattc aatctgctca acttttgata caaagacacg actacgatct
     6961 tgatgccatc ttccgggctc tgcagaacac acatactcga ttagaacgtg aatatgagca
     7021 ggcccaacac cttcgtcaag aggcacaaca cttgtttgat gaggcggaag tcactcgcaa
     7081 tcaattgcaa gcccaggaac gagaagaccg gcaacgatat cggaagcagt ggcaacaaga
     7141 attttccaaa gcacaacggc aacttaatca aatcatcaat gacttaaaag cggacaaaac
     7201 actctcgaag gttcgttcta tccagcaaac cattggttcg gtgaatcaag aaatactctc
     7261 ccgcctcccc cataattccc tggaatcatc tgagcctcca cacgaagggg accttgtgga
     7321 aattgatcca ttggggacga tcgggatctt gcaggaaagt cttgaaggga aaaagcaggt
     7381 atccattcgt gtgggtaccc agacaattaa aaccgcccct tcagccgtac gtcgagcaac
     7441 agcttcttcc ggcaataaat ctttgtcagg gccgcaacaa ttgagtcgga aacggtctct
     7501 ctccacaatt tccactgaga cgagccaccc aagctctcat caggcagctg ggcaatatca
     7561 acaggaactg tgtattcgtg ggttccgtct ggacgatgcg atggagatga cgcttgcggc
     7621 cctagaccat gccctggtca cccaagccaa atatcttaaa gtcatccatg gccagggttc
     7681 tggcgcactc aaaacaggaa ttcgaaattt ttgtcaatcc tctccctata ttcaaagctt
     7741 tcgacccggt gatccagccg agggaggaga cggtgtgacc gtgatcgaat tacgataaag
     7801 gaattggtgg agcgtgatta tcgagtccaa tctgtacatt tctgataaag ccctcatgct
     7861 tagggcggcg caaggggctc cctgtcgtga gtgattgaaa ttcatctttt gtcagattcg
     7921 ccaattcagg taattgggga tgtaatgtcc atggaaatgg ttggaatcct gcctccgtag
     7981 tgacttttgc attcacatta aaaggacaaa tatccaaaca atcgtcacaa ccaaaaatcc
     8041 tattgcctat ttttttcctc agcccagctg gcatgtcatc tgccctaccc cggtattcaa
     8101 tcgtcaaata cgagatgcat cgttccgcat caaggagata gggttccacg atggcgccgg
     8161 tggggcaagc ctgaatacag agcgagcagg ttccacataa atccatggcg ggttcgtcac
     8221 aatccaacgg cagggtcgtt agaatttctc cgagcaacag ccaagaccca aactcggtgg
     8281 aaacaatatt ggtgtgtttg ccgatccagc caatcccggc ctcctgggcc cacggttttt
     8341 ccatgatggg cccggtatct acataactcc gcgtctgaat atccggtacg tgtgagtgaa
     8401 gaaagttttc taactgtcta agccggtcct tcatgaaccc gtgatagtcc tttccccagg
     8461 catatcgggc aattcgtcca gccccctttg actcatccgg agtatgatcc gtgtaataat
     8521 tcatccccac gactaccagg gattggcaac ctggaagaac ttcttctggg ttagagcgac
     8581 gtttcggatc cttggctatc cattccatag ttgcatgaaa gcccaagtcc agccattttt
     8641 tcagtcgttc ccataatcgc aaggtaattg attcctcagg ctgggaaatc gtattttgag
     8701 ccgcaggtaa gggtatgggg ggtagaggcg gcggcagacg agcaacccca acagcatcaa
     8761 agcctagtcg gcgggcctct tccttgattt gatttgccag gatcgaccga ggagacatga
     8821 ttactcctcc tctgtataaa ttcaagattt acttttgtcc agggattcct ggattagcag
     8881 gaggaacatt gttcgcacaa ttaaacatca cgataaatct tcctgtacat tgctcggtgg
     8941 tgcaatcctg gcatgatccg acaattgaag ttttccagtc ggtttgtttc tcatcaaatt
     9001 cacaagaagt tccccctcct ttggaaatat caacccacgg ttcgggatat ccaggaaact
     9061 gggcaacagc acagccgttg ggaatgggaa cttggcattc agccttggcc tcgcctttta
     9121 ccataccaat ggtacagttc gcttctgtgc gtgaccgaga gtccgctgct tcccccttat
     9181 tgataagtcc aagcaagccg atttcggtca ggagaaaaac cacaataccc gacatcaata
     9241 gccgactggt ttttgtttga ccaattatta ctttgattgt gtttgagtat aaattcatag
     9301 ttagcccaat ctggagcaaa ggatgaggat tactgcacga atacaataga aaatttttat
     9361 aaagatggac ttctttatac cacatccctg aaacaatctg tcaaaaaacc ctctccttat
     9421 gcagggcggc tatcaattaa ctgattcgta agatgactta gttcaatgaa tttttctgtc
     9481 tgatcggcga aagaattata gattcagaaa tagaaaaccc cctggaggat cgaatcctcc
     9541 agggggtttt cattatattc gtgagtcgga ttttagaatc cgcccatgcc gcccattcca
     9601 cccatgccgt ggtcatggcc gccaggcatc ccagcacctt ctgactttgc ttctgggatt
     9661 tctgtaacca tgacttctgt ggtaagcatg agtcctgcaa cgctggcagc gttttgcaac
     9721 gcgcaacgtg cgaccttggt gggatcaatc accccggctt caaccatatc aacaaactct
     9781 ccggtgcctg cattgtagcc tcggtttgtg gtttcacctc gaaccttttc cacaataatg
     9841 gaaccttctg cgcctgcatt gatggaaatt tgtcgaatag gttcttccag ggaacgcttg
     9901 acgatattta cgccgacttg ttgatcgtgg tctaatttca ttttatccaa agccggaaca
     9961 caacgaagga gagccacgcc cccgccagga acgatacctt cctctactgc ggctttcgtc
    10021 gcatgcaggg catcttccac acgagctttc ttttccttca tttctgtttc ggttgcggcg
    10081 cctacattaa ttacagccac gccaccaacc attttcgcca atcgctcttg caatttttcc
    10141 cgatcataat cggatgtcgt ttcctcaacc tgtgttttaa tttgcttgac gcgaccttca
    10201 atccgtttgg ggtctccagc tccttccacg atcgtggtat tgtccttgtc aatattcacc
    10261 cgcttggccc gaccgaggtc tgtcagcttc acactttcga gtttgagtcc tacttcctca
    10321 gaaatcactt gcccgcccgt cagggtggca atatcctcca acatggcttt ccgtctatca
    10381 ccaaagccag gagccttaac tgctgccaca ttcaacgtgc cacgaagttt attgactacc
    10441 agtgttgcca gggcttcgcc ttcgacatct tcagcgataa tgactaacgg ttttcccatt
    10501 ttcgcaactt gctccagaat agggagtaag tctttcatgg cgctaatttt cttttccaca
    10561 atcagtaaga acgcatcttc caacgaagct tccatgcgat ccgcattggt cacgaaatag
    10621 ggagagatgt acccgcggtc aaactgcatt ccttcgacta catctaagga ggtcgacatg
    10681 gatttggctt cttctacggt gatgacgccg tcttttccca ctttatccat tgcttcggcg
    10741 atcaggtcac caatggtgtg atcattattg gcggagattg aaccaatttg agaaatttcc
    10801 tttttggtct ggcagggctt gctgaacttt tttaattctt caatcgcaac ttcaacggct
    10861 ttatcaattc cccgtttaat ctccattgga ttggcgcctg ccgtgatatt tttgacccct
    10921 tcacggtaaa tggcctgtgc caatactgtc gcagtcgtgg ttccatcacc agcggtat
//
LOCUS       DATIAL010000225         3826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1330505, whole genome shotgun sequence.
ACCESSION   DATIAL010000225 DATIAL010000000
VERSION     DATIAL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330505"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VLA60_07035"
     CDS             complement(<1..370)
                     /locus_tag="VLA60_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09150.1"
                     /translation="MNAVPVCSAWLLCISFLVVGLTTSVTSAGECHPIVATVVSVQGR
                     VEVRRVNVEQWQPVSSHDSFCVGDRIRVGDGSRADIALANRAVLRLNQNTTITLGGIQ
                     KEQTVLVVLIESATDVFTRAI"
     gene            complement(599..796)
                     /locus_tag="VLA60_07040"
     CDS             complement(599..796)
                     /locus_tag="VLA60_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09151.1"
                     /translation="MQQETVIFNRQIIRLFKGILKAWEQWVDEVEFVKSHKGRDVETT
                     DLAPRIVGKNEGDPNDRTMNP"
     gene            complement(1819..2610)
                     /locus_tag="VLA60_07045"
     CDS             complement(1819..2610)
                     /locus_tag="VLA60_07045"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HSF09152.1"
                     /translation="MNIRVLGAHGSDMLLNGPTNPHICRSVGFLVNEELMVDAGTLAS
                     GLTLEEQKRITHILLSHLHLDHVKGIPPLVDNMSGRVDHQIVVASLPSVLVGLQKHVF
                     NDLVFPNFFHLPKSQYSMLRGEELEPKREWRLPGRVRVTPIPVNHTVETAGFIIQDDE
                     VAWIYSGDTYVTENLWQEAARIPNLKGVFIEVSFPDSMKDMASRSKHLTPTLLAQEYR
                     KIGKPDLPLYVYHMKPTVRDQIIDEISHLDIPHVTILKEGQEVKF"
     gene            complement(2896..3564)
                     /locus_tag="VLA60_07050"
     CDS             complement(2896..3564)
                     /locus_tag="VLA60_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSF09153.1"
                     /translation="MVMYKQAIKNVPLFSELTDQELNLLAASGSRQKFPDKNVIFQEG
                     DSGEVLFIILSGKVKVLLTGKNGQEFILSHLGPGNFFGEMAILESAPRSASVITVEPS
                     EFFLLGRKALTELLKHHPDIAMKILKNLSQRLRKVSEQVRSLVMFDMYGRVGRCLLDL
                     AEFQDGVETHGQLLISNRPSFQELANMVGCSRETLSRTLKALKENGSLTVTRNTIYIN
                     RLWE"
BASE COUNT         1000 a          905 c          867 g         1054 t
ORIGIN      
        1 gaatggcacg ggtgaaaaca tcggtggcac tttcgatgag gacgaccaga actgtctgtt
       61 ctttttgaat gcctcccaga gtgatggtcg tgttttgatt caaccggagt accgctcgat
      121 tggcgagggc gatatccgcc cggcttccgt caccaacccg aatgcgatcc ccgacacaga
      181 aactatcgtg ggaactcacc ggctgccact gctcgacatt cacgcggcgc acctccactc
      241 tgccctgaac ggacacgacc gtcgccacaa tcggatggca ttccccagcc gatgtgaccg
      301 acgtggtaag cccgacaacc aggaagctga tgcacagaag ccaagcggaa cagacaggga
      361 ctgcattcat ggtcatattt cacccgggtt tgtatggaga aggggtgctc tgtgttctcg
      421 tctttcgatg tgtgggtgag cggaaaccgg ggaattttgg gtgggaatat tcatacagta
      481 tttcactcct ctttctctca gagtttccag caacgatccc ggggcttgga ttcggaggct
      541 ttgacgacac gaggcgtcga atgacgcgag gggagaaagc gtacgaaggt tagagtcgtt
      601 acgggttcat ggttctgtca tttggatctc cttcattttt tccgacaatt cgtggcgcca
      661 aatctgtggt ctcaacatcg cgccctttgt ggcttttgac aaattcgact tcatccaccc
      721 attgttccca tgctttgaga atacctttga agagacggat gatctgtcgg ttaaagatga
      781 cggtttcttg ttgcatgggc aagttccttc cctcacattt ctgcacaacg ggtcatcgaa
      841 tcgttcctat gcctcaacga cttgacgaat cctgtttttc aacgtctgag acttgtgagg
      901 aggagggagg atccatggtc cgcaccttct acctacctcc gaatcccact cctttcgtca
      961 tgaattcctt ggcgacagca agaaaccaaa cccagagagg cgggttcatt aataagaaac
     1021 gattgcgtgt tcagaaacac gagcccaagc tactctctct ctctctataa atgtcgccat
     1081 acctacaatc cttcctcctg gattaggaaa tcgtgccatg gaaatgattg gggctcttgc
     1141 cttttgtgat tttgatcacg ttagttgtca ttgaggcaac ttcaatacca tccatatgtt
     1201 gggtgccctt accggtttca ttgaagataa ccaattggct gaaaacagaa atttccgttt
     1261 tcaaaccatt tcaactgttg ttatgccatt tcagacacca cagaagggta tccatttgga
     1321 tacataagtt atctatttag atacatgtct tggttgacga ctccctgtct ctgaatgtcc
     1381 caaggtcgat cattaaggtc tatgtattgt cttttagtcc gaatgctcca tggtctctct
     1441 caaattattc aggacaggat tgatggattg agattcttta tctgaaagag gaaatgaaaa
     1501 atccaaatga tccaacgcag atattggaat tgtcggaggt gttgtaggtt tgaataagga
     1561 aggactacac cacgccaatg cgatagtctg aaaggcggtg acgcttggga aatgaacgtg
     1621 aggaatagaa gagttaaaag aaaacggtca ttggtaggag tctgtcggac tttgagaatc
     1681 gatagcgaaa atgtggctga gcgagggcag attttgacga ttttgccggc ccatagtggg
     1741 actatgggcc aaggaagcgg cgaaatatgg accgcttagg cacttttgca gccgattcat
     1801 ccgaagtccg acaggcttct agaatttcac ttcctggcct tctttcaata tcgtgacgtg
     1861 agggatgtcc agatggctta tttcatcaat gatttggtct cgtacggtag gcttcatatg
     1921 gtagacgtat aagggcagat caggtttccc gatttttcga tattcctggg ctaaaagagt
     1981 tggcgtgaga tgtttacttc gagaggccat atctttcatt gaatcgggaa aggatacctc
     2041 aataaagact ccttttaaat ttggtattcg ggccgcctct tgccaaaggt tttccgtcac
     2101 ataggtatct ccactataaa tccaggccac ctcgtcgtcc tgaataataa accctgcggt
     2161 ttcgacggta tggttaacgg gaatgggggt tactctaacc cttccaggca agcgccactc
     2221 gcgtttgggc tccaattcct cgccacgtaa catggaatat tgagatttgg gaagatggaa
     2281 gaaattcgga aataccaaat cgttaaagac atgcttctga agtccaacta aaaccgaggg
     2341 taaacttgcc accacgattt gatgatccac ccgcccggac atattatcga caagtggggg
     2401 aatgcctttg acgtgatcta aatgcaagtg ggaaaggaga atatgtgtga tccgcttttg
     2461 ctcctccaag gtaagtcctg atgctaatgt tccagcgtct accatcagtt cttcattaac
     2521 caaaaacccc acgcttcgac aaatatgcgg attggtcggt ccattcaaga gcatatcaga
     2581 tccatgggcc cccaataccc ggatgttcac aaaaaatccc ttgcatgaca gcaatgatcc
     2641 atacccttgc cttcctggtg tgccatattc aaaaaatgca gggatgagcg acccatttca
     2701 agtcgtggaa tcctaggggg taaccgcctt ttcgatggaa agacaatccg aggatctttt
     2761 ctacttttta atttatccca ttccaggcct cctgtctgtg atattcatca cattcttggt
     2821 gtgattctcc tcacacttct tcaacgccat attttcttaa aatcatcaaa attaccaaac
     2881 caaatttata gggttttatt cccataatct attgatatat atcgtgtttc tggtaaccgt
     2941 aagacttccg ttttctttaa gagccttaag ggtccgactc aaggtttccc tggagcatcc
     3001 taccatgttg gctaattcct ggaaggatgg acgattggaa atcaataatt gtccatgagt
     3061 ctccacccca tcctggaact ctgctaaatc aagaagacag cgacccacac gcccatacat
     3121 atcaaacatt acgagactgc gaacttgttc gctgactttt cgcaaccgtt gggaaaggtt
     3181 tttaagaatt ttcatagcaa tgtctggatg gtgctttaaa agttccgtca aagcctttcg
     3241 tcccaataaa aaaaattcac tcggctccac cgttatcact gatgcggatc gaggagccga
     3301 ttccaggatg gccatctcac cgaaaaagtt ccctggacct aagtgcgaga ggataaattc
     3361 ctgtccgttt ttcccggtta acagcacctt aactttgcct gacaaaataa tgaataacac
     3421 ttcgcctgaa tcgccttcct ggaatatgac atttttgtca gggaactttt ggcgactccc
     3481 ggaagcggcc aacagattga gttcctgatc cgttaattcg gaaaagagtg ggacattttt
     3541 aatcgcttgt ttgtacatca ccatgacagg aaatttcctg gcgagtcatt cttcccttgg
     3601 attgagcagg gattagttct ttttcgaaaa aagcacaata attccgacat cagcccattt
     3661 taagatttca aatggagcca tcatgcaaaa atatttcaat aaagacaaca cccttgtgca
     3721 tggcgaccac tatccactga gcctccaggg atacacactt gctcgatcaa actggtgacc
     3781 atcgccggga tcatcgtcag gagggccaaa tcttcctcat gatttc
//
LOCUS       DATIAL010000226         5817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1115364, whole genome shotgun sequence.
ACCESSION   DATIAL010000226 DATIAL010000000
VERSION     DATIAL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115364"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="VLA60_07055"
     CDS             complement(<1..1765)
                     /locus_tag="VLA60_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09154.1"
                     /translation="MEPSGYRTFPVSFWLPRVLWSIVACGVLAVGILLFTLTVLSDTK
                     WVKESLISMLEGTVGGPIQIETLNLNLFPSPAILLSGLSFQTHDPGIVAIHAIQVEVG
                     IGWRSLWDKKLFITHAVIDQPELTLEVPLVTQEEQPVTRQFPSIQEFTIRNGTLHLIH
                     TSPLPEQDMGLDWEAIQLSVMDVESEGSALINLSARIPEPQPSSTLTLKGTMTALDQE
                     DTSSNQKELSGFPAVKVQGQVEISQFHLGHLVQFLRGRALEAPIQTQATFQGNFSLTI
                     QKEQDLLDFQSFQVALDEWSFTGQGSIANVLHDSPWLKVSGVTQPIAIERLPDLIPDD
                     WMPTDFQTFLREHQIAGRMALQGGSIGGPLDGNGSWEAKGTVALEEGQYLPAPGQPLI
                     TNVGATVTFAPSVIQISQVRGTITPLTVTAPEAKLAREEEIVRLSIPTFRISEGDWNL
                     NGTAAFTNNQNAPPTLTISGSALPISIQRLANILPEPWRPDSVQTMLTERDMDGEMEL
                     LTGTVKWIGDEANTVISEGVIRVANGHVLVDPHHPPMTEISGAVVFDDNLVRVLDAKG
                     TMESSNLLVKEATLELKNPDIF"
     gene            complement(2005..3039)
                     /locus_tag="VLA60_07060"
     CDS             complement(2005..3039)
                     /locus_tag="VLA60_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HSF09155.1"
                     /translation="MTISLLSWIGILFCLTGSAMCSGLTLGFFSLSRIHLELLAKQGD
                     KAANTILQIRQDANFLLATLLWGNVAVNVLLTLLAEDQMVGLLAFFFSLFGITLFGEI
                     LPQAYFSRNALRLGAKFTPVVHLLQFIFYPVAKPSALLLDRLLGKEGITWLKEHELDT
                     LIKIHAQAPETDISGVEGHGASNFLNLDDISALEEGSPLHPQSIISLPFDGKNVRFPS
                     ITPSASDHFVQQIHGSKEKWVVITNEEHVPQVVLDADGFLRELLVEHRDKPLRHCHRP
                     IILEHTDMPLGDLLTQFKVNPEHLEDDVIDHDLVLIWTPTHKRIITGADILGRLLRGI
                     AKRNPLVNFR"
     gene            complement(3178..4482)
                     /gene="mgtE"
                     /locus_tag="VLA60_07065"
     CDS             complement(3178..4482)
                     /gene="mgtE"
                     /locus_tag="VLA60_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HSF09156.1"
                     /translation="MTTPATTEQMHQAFIAKFPDEAAHVFESYDPQEVIPILADLPPT
                     LIAKVLSVMSPSLAADLLGIIPQPLLLSALPHLQPAVAASLLQRMPDETRVAILDALP
                     LHVSADIEPFMEYSEDSVGMLMDVKFFALPEDLTVEEAIQQVRTHDTQHLNEIYIIDR
                     RQILVGVLSLRDLFLAPPKKSLAVLMKRELPTIHPLENQEQVVEICNEWKVLTIPVTD
                     LDGRLLGIISSQDIIQVEKEEATISMQTMVGASKDERALSPPGFAIRKRLPWLQINLL
                     TAFLAAFVVGLFEDTIARFTALAVLLPVVAGQSGNTGAQALAVVMRGLALRDIRPSQW
                     LRVTLKESYVALANGVAVAATTCTAVFVWSQSWGLTMVIGVSMIISMVMAGFSGAIIP
                     IILRSLKQDPAQSSSIILTTVTDVAGFFSFLGLATLFSALLE"
     gene            complement(4479..5342)
                     /locus_tag="VLA60_07070"
     CDS             complement(4479..5342)
                     /locus_tag="VLA60_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09157.1"
                     /translation="MNLTQQLRETFIQEHPLEAARYVEELPANTAGEMLHTMEPQDIA
                     AFLEYCLPGPTAEILKQYPPATSAAILSRLSSRSARAVLRQYDSSTQTSLLDQVDPAI
                     GAHLRRSINLPDHTAGSLADPHVLTLPPDLTVAKALQRVTQTVGQAIYYLYILDHQTI
                     LRGVILMKELLGAEPTITVSSIMQQDVKAIPASANALDIVAHPAWAQYDSLPVIDQDR
                     TFIGALRHRTLRQFLQSRSGEYQPAFLSDALLQLWEAYSLSGIGLMTALGESLSTSTP
                     PTPPRKEQETP"
     gene            complement(5347..>5817)
                     /locus_tag="VLA60_07075"
     CDS             complement(5347..>5817)
                     /locus_tag="VLA60_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HSF09158.1"
                     /translation="KIGMATLILVLGLLATNFVRDLISVACDSSGIRQGTIIAQTVYI
                     AATLLVLVTAINELGIDTSLLNQIIVILIAGLIAGAALSFGIGSRSAVKNLIAAHYIQ
                     PIVRIGERIQVGAYNGTVTAITPMVVVLDTAKGRVVIPAAQFTEVTSILSPTEG"
BASE COUNT         1404 a         1385 c         1655 g         1373 t
ORIGIN      
        1 tgaagatgtc cgggttcttc aattccaatg tcgcctcttt cacaaggagg ttggatgatt
       61 ccattgtgcc ttttgcatcc aatacccgga caagattgtc atcaaagacc accgctccag
      121 agatttccgt cataggggga tggtggggat cgaccagcac atgtccgttg gccacacgga
      181 tgactccttc cgatatcaca gtgttggctt catcccctat ccatttcact gtgccggtca
      241 gcagttccat ctcaccatcc atgtctcgtt cggtcagcat cgtctggaca gaatcaggcc
      301 gccatggctc gggtaggatg ttggccaggc gttggatgga aatcggcaag gccgatcctg
      361 agattgtcag ggttggggga gcattttgat tgttggtgaa cgcagctgtc ccattgaggt
      421 tccagtcacc ctcggaaatc cgaaaggttg ggatcgaaag gcggacgatc tcctcttcac
      481 gagcaagttt ggcttccggg gctgtcacgg ttaacggagt aatggttccg cggacctgag
      541 aaatctgaat gacagaggga gcaaaggtga ccgttgcgcc aacattggta ataagaggtt
      601 ggccaggggc gggaaggtat tgaccttctt ccaacgcaac ggttcctttc gcttcccagg
      661 atccatttcc gtccaggggc ccacctatcg atcccccctg aagcgccatc ctaccggcta
      721 tttggtgttc tctgagaaaa gtttggaaat ccgttggcat ccagtcatcg ggtatcaggt
      781 cggggagccg ttcgatggct atgggttgtg ttactcctga gactttcaac caaggtgaat
      841 cgtgaaggac attcgcgata ctgccctggc cggtaaacga ccattcgtcc aaggcaacct
      901 gaaaactttg aaagtcgagg agatcctgtt ctttctggat agtcaggctg aagtttcctt
      961 gaaaggttgc ttgtgtctga atcggtgcct ctaacgcacg tcccctcaga aactgaacga
     1021 ggtggccaag atgaaattgt gaaatttcaa cctgcccttg gactttcacg gcgggaaaac
     1081 cggaaagttc cttttggttg gaagaggtgt cttcttggtc gagggcggtc atcgttccct
     1141 tgagggtgag ggtcgaggaa ggctgaggtt ccggaatccg agccgagaga ttgatgagtg
     1201 ccgatccttc tgattccaca tccatgaccg aaagttgaat cgcttcccaa tcgagtccca
     1261 tgtcctgctc tggcagcggt gaagtatgaa tcagatggag ggtgccgtta cggatggtga
     1321 attcctgaat agaaggaaac tgtctggtga ccggctgttc ctcttgcgtg accagaggaa
     1381 cttcaagcgt caattccggt tgatcgatca cggcgtgagt aatgaacaat tttttgtccc
     1441 agagcgatcg ccacccgatg cccacctcca cctggatggc atgaatggcc acaatgcccg
     1501 gatcatgagt ttggaaggat aaacccgaca agaggattgc cggagaagga aacagattca
     1561 ggtttaacgt ttcaatctga atgggcccgc cgaccgtacc ttccaacata gaaatcaagg
     1621 attctttaac ccacttggta tccgataaga cggtaagggt aaagagcaga atccccacgg
     1681 cgagcactcc acaggccacg atactccaca gaacccgtgg aagccaaaat gacactggaa
     1741 aggttcgata tccggacggc tccatcactc tcattcttcc ctgaatgact atgaggggaa
     1801 atgattgatc atgatcgagg actattcatc tgtcgcgtgt tcctgattcg tggatgatgc
     1861 ggtggattca tcaaaaggcc cccagtacat tcgttgacac acgtgattgt ctcaaaccat
     1921 ggcgtggaat gaatacatgt aattgtgcct catgtttcat tttatccgaa aatttcttat
     1981 gtcagtcgtt caggccctat ccttttacct gaaattgacc agtggatttc gtttggcaat
     2041 cccgcgcaag agtctcccta agatgtcggc gcccgttata atccgtttgt gggttggtgt
     2101 ccaaatcaga accagatcgt ggtcaatgac gtcatcctca aggtgttccg ggttgacctt
     2161 gaattgcgtc agaaggtccc cgagcggcat atctgtgtgc tcaagaatga tgggccggtg
     2221 acaatgtcga agaggtttgt ctcgatgttc caccagaagt tcccgcaaga atccatccgc
     2281 atccaatacg acctgaggga cgtgttcttc attcgtgatc accacccatt tttcctttga
     2341 cccatggatt tgctgaacaa agtggtcgga ggcagaaggc gtgattgagg gaaagcgcac
     2401 atttttcccg tcaaagggga gcgaaatgat gctttgagga tggagtggcg atccttcctc
     2461 aagggccgag atatcatcaa gattgagaaa attggatgct ccatggcctt cgactccgct
     2521 gatgtctgtt tcaggcgctt gcgcatgaat tttgattaag gtatcgagct cgtgctcttt
     2581 aagccaggtg atcccttctt tcccgagcag gcgatcgaga aggagagccg atggtttggc
     2641 cactggataa aagataaatt gcaagagatg aaccaccgga gtgaattttg ctcccaatct
     2701 caatgcattt cttgaaaaat acgcttgggg aagaatttct ccaaaaaggg taatcccaaa
     2761 caacgagaaa aaaaacgcca ataggccgac catttgatct tcggcgagaa gcgtcaggag
     2821 gacattgacg gccacgttac cccagagaag tgtggcaaga aggaagttgg catcctgccg
     2881 gatttgcaag atcgtgttgg ccgccttgtc accttgttta gctaagagtt ccaggtgaat
     2941 gcggcttaag ctgaagaacc caagggtcaa gcctgagcac atggccgacc cggtcaaaca
     3001 gaaaaggata ccaatccaac tgaggagtga aatcgtcata tctgaaagtc tatacgaccc
     3061 aatatgtgtg agagatatat cttacatacc ttgtcccgaa acagggatga ccgggtgggg
     3121 gtggtgtatt gggggttgga caattggcta gattaccgga tagtttcacg cgggtgccta
     3181 ttctaagaga gctgaaaaca gggtcgcgag ccccaaaaaa ctaaaaaatc ctgccacatc
     3241 ggttacggtg gttaagatga tcgatgatga ttgagccgga tcttgtttta gggatcgaag
     3301 gataatggga atgattgccc cggaaaatcc ggccatgacc atggagataa tcatggagac
     3361 gccgatgacc atggttaatc cccaggattg gctccagaca aatacggccg tacaggtcgt
     3421 cgcggccact gccactccat tggccaaggc gacataggat tctttcaagg tgacgcggag
     3481 ccattgggaa ggacggatat cccgcaacgc taatccacgc atgaccaccg cgagcgcttg
     3541 agctcctgta ttccctgatt gcccggccac gacggggagc aaaacagcta aagccgtaaa
     3601 tctggcgatg gtgtcctcaa ataaccccac cacaaacgct gccaaaaagg ctgtgagcag
     3661 attgatttgc agccagggga gacgttttcg aatggcgaat cccggcgggg acagggctcg
     3721 ttcgtccttg ctggcaccca ccatggtttg catgctaatg gtggcctctt ctttttcgac
     3781 ctgaatgatg tcttgactgc taatgatccc gaggaggcgg ccgtccaaat cggtgaccgg
     3841 aattgtcagc accttccatt cattgcaaat ttccacgact tgttcctgat tttccagggg
     3901 atgaatggtc ggtaattctc gtttcatcaa gaccgcaagg ctcttctttg gaggggctaa
     3961 aaacagatcc ctgagggata ggacccccac gaggatctgg cggcgatcaa tgatgtagat
     4021 ttcattgaga tgctgggtgt catgggtgcg aacctgttgg atggcttcct cgacggtcag
     4081 gtcttccggg agcgcaaaga atttgacatc catgagcatg ccgaccgaat cctccgaata
     4141 ctccataaat ggttcaatgt cggctgagac gtgcaagggt agggcatcca aaatggctac
     4201 acgagtctca tcgggcattc gctggaggag agaggcggcc accgcgggtt ggaggtgagg
     4261 aagcgcactc aggagtaacg gttgcggaat gattccgagc aggtcagcgg ccaatgaggg
     4321 tgacatgacc gagaggacct ttgcgatcaa agtgggggga agatcggcaa gtatggggat
     4381 cacctcctga ggatcatagg attcaaacac atgcgcggcc tcatccggaa atttggcaat
     4441 aaatgcctga tgcatctgtt cagtcgttgc aggtgtcgtc atggtgtttc ctgttctttc
     4501 cggggagggg tgggcggtgt cgatgtgctc agggattctc ccagggcggt catcaagcca
     4561 attcctgaga gggaatacgc ttcccacagt tgcaggaggg catccgagag aaatgccggt
     4621 tgatactcgc cggaacgtga ctggagaaat tgcctcagtg ttcgatgtcg aagcgcgcca
     4681 ataaacgtac ggtcttgatc aatgaccgga aggctgtcgt attgagccca tgcggggtgg
     4741 gcgacaatgt cgagggcatt agccgaggcc gggatcgctt tcacgtcctg ctgcatgatg
     4801 gaggacacgg tgatcgtcgg ttcggctcca agcagctctt tcatgaggat gactccgcgc
     4861 aggatcgtct gatgatcaag gatgtataaa tagtagatgg cttgcccgac ggtttgggtc
     4921 acccgctgta aggctttcgc gacggtgagg tccgggggaa gcgtcaggac atgcggatcg
     4981 gcgaggctgc cggcggtatg gtccggcaaa ttgattgatc gccgaaggtg tgcaccgatg
     5041 gccgggtcaa cctgatccag gagagaggtt tgtgtggatg aatcatactg tcggaggact
     5101 gcccgtgcgg aacgggagga caaccggctg agtatggccg cgctggttgc cggtggatac
     5161 tgtttaagaa tttcggccgt tgggccggga agacaatact ccagaaaggc ggcgatatct
     5221 tggggctcca tggtgtgcaa catttctccc gctgtattag ccggaagttc ctccacgtag
     5281 cgtgcggcct ctaatggatg ttcctggata aaggtttccc gaagctgttg ggtcaaattc
     5341 atcggcctat ccttccgttg gagaaagaat gctggtgact tcagtaaatt gcgcggcagg
     5401 aatcacgacc cgtcctttcg ccgtatccag gacgacgacc atgggagtga tggctgtgac
     5461 ggtgccattg taggcgccga cctgaatcct ttccccgatt cggacaatgg gctgaatgta
     5521 gtgggcggca atcaagttct tcacggctga acgagaaccg attccgaatg ataaggcggc
     5581 tccggcaatg agtccggcaa tgagaatgac aatgatttga ttaagcagcg acgtgtcaat
     5641 tcccaactca ttgatggccg tcaccagaac cagcagagtc gcggctatat agaccgtttg
     5701 cgcgatgatg gtgccttgcc ggattccgga agaatcacac gcaacagaga tcaggtcgcg
     5761 tacaaagttt gtggccagga ggcccagaac gagaataagg gtagccatgc cgatttt
//
LOCUS       DATIAL010000227         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_40549, whole genome shotgun sequence.
ACCESSION   DATIAL010000227 DATIAL010000000
VERSION     DATIAL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40549"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..449)
                     /locus_tag="VLA60_07080"
     CDS             complement(42..449)
                     /locus_tag="VLA60_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09159.1"
                     /translation="MKIGGIQQESQEITENEPGASGVVMYVAERIQDETLVLMISGRV
                     TFYSRKVFQGIVKTARFSGAKHIIFNMQGVTFMDSTALGSLVLAYLNLNEGNVAMSLV
                     EPKPPVKALLEDANFPSLIPTYPTEEIAFQAIK"
     gene            789..>2160
                     /locus_tag="VLA60_07085"
     CDS             789..>2160
                     /locus_tag="VLA60_07085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HSF09160.1"
                     /translation="MMENKPLNQQRVIPTARPESLQLHSRQQNTSKQFPLHSLLKEHF
                     GFTAFRPLQEEIIRDVLAEHDVVALLPTGGGKSLCFQLPALARSGLTIVVSPLIALMK
                     DQVDGLQANGIAATFLNSSLATQESQARLRGLHHGDYRLLYIAPERLMVPGYLGLLHN
                     WQVNLLAIDEAHCISEWGHDFRPEYRRLVELRDRVPSLPIIALTATATKRVQEDIVTL
                     LRLRDPACYVASFNRPNLTYQVQAKNNPFGQVLAFLRTRPKDCGILYCQSRKTTESVA
                     DRLQHHGIKAQPYHAGLSAPERTRHQELFLRDEIQVICATIAFGMGMNKPNVRFVIHY
                     DLPKNLESYYQETGRAGRDGLPSECVLLFSAGDTVKQQRFINEKPSIQEQQIAHKQLQ
                     EMVQYAESATCRRRTLLQYFGEKVESERCGGCDNCLSPRDIFDGKDAAQTVLSCVEEI
                     RGESGFS"
BASE COUNT          570 a          581 c          517 g          492 t
ORIGIN      
        1 actaattaga caatgaaaaa aagaaaggca ccgcaaaaaa tctatttgat ggcttgaaag
       61 gcaatttctt cggtgggata cgtcggaatc agactgggaa aattcgcgtc ttcgagtaaa
      121 gctttgactg gcggttttgg ctcaacaagg ctcatagcca cattaccttc attcaagtta
      181 aggtaggcta ataccaagct ccctaatgcg gtgctatcca taaacgtgac tccctgcata
      241 ttaaaaataa tatgcttggc ccccgagaat ctggccgttt tcacaatgcc ttgaaaaact
      301 tttctggagt aaaaggtcac tcgccctgat atcatcagaa caagcgtctc atcttgtatc
      361 cgttcggcaa catacataac gacacccgag gctcctggtt cattttcggt aatttcctga
      421 gattcttgct gaataccccc gattttcacc cgatgtcgaa cgtcctattc tgaccccgaa
      481 agagagactt cgaatggaag aagcccttca cagctctaaa catattggat ggaatggaaa
      541 ggaagaaaat gctgaaaaat cgaagaagtc aagaaaaaaa gcagaaagtc accattgaaa
      601 tatttcaatg aaccccagag atatttggag gaaatcatca tgatgaggcg aagccatgta
      661 gccggatgtc atttcaccgc ggaaaacccc ttgtgtcata cccgcatatc cctccgccca
      721 ccaccggatt gtgtccggaa cgttttccga attattcagc accttactca acgttcgccg
      781 ttattcacat gatggagaac aaaccactca accagcaaag ggttatcccg actgcccgtc
      841 cagagtcgtt gcaattacat tcaaggcaac agaacacctc aaaacaattt ccgcttcact
      901 ctctgttgaa ggaacatttt ggtttcaccg cctttcggcc gttacaggaa gagatcattc
      961 gggacgtcct cgcggagcac gatgtggttg cgctgttgcc aactggagga gggaagtccc
     1021 tgtgctttca gttaccggca ctggcccgat cgggcttgac gatcgtcgtc tccccgctca
     1081 tcgcattgat gaaagatcaa gttgatggac ttcaggccaa tggcattgcc gcaacctttc
     1141 tgaactcctc cctggccaca caggaatcgc aggcccggtt gcggggcctg catcatggtg
     1201 actaccggtt gctctacatt gcgccggaac ggctcatggt ccccggttac ctcggcctcc
     1261 ttcataattg gcaggttaat ctgctggcca ttgatgaagc ccattgtatc agcgaatggg
     1321 gtcatgattt ccggcccgaa taccggcgac tggtggaact gcgagaccgt gtcccttccc
     1381 tcccgatcat cgcgctcacc gccaccgcca cgaaacgggt acaagaggac attgtgactc
     1441 tcctccgact gcgcgacccg gcgtgctacg tggccagttt caatcggccc aatctgacct
     1501 atcaggtgca ggccaagaac aacccctttg gacaagtcct ggctttcctc cggacacgcc
     1561 ccaaggactg cgggattctt tactgtcaga gccgaaagac aacggaaagt gtagccgacc
     1621 gattacagca ccatggcatc aaagcacagc cctatcatgc gggactaagt gccccggaac
     1681 gcacacggca tcaggaattg ttcttgcgtg atgagatcca ggtgatctgc gccaccattg
     1741 ccttcgggat gggaatgaac aaacccaacg tgcgatttgt catacactac gatttaccca
     1801 aaaatctcga aagttattat caggagaccg gccgcgccgg ccgcgatggc ttacctagcg
     1861 agtgcgtact gctcttcagc gccggagata cggtaaagca gcaacggttc atcaatgaga
     1921 aaccgtccat ccaggaacaa cagattgccc acaaacaatt acaggaaatg gtgcaatatg
     1981 cggaatctgc cacatgccgg cgtcgcacgt tgctccagta ttttggagaa aaggttgaat
     2041 ccgaacgttg tgggggttgc gacaactgtc tgtcacccag ggatatcttc gacggcaaag
     2101 atgccgccca gaccgttctc tcctgcgttg aagagatacg aggagaaagc gggtttagcg
//
LOCUS       DATIAL010000228         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_793505, whole genome shotgun sequence.
ACCESSION   DATIAL010000228 DATIAL010000000
VERSION     DATIAL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793505"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VLA60_07090"
     CDS             <1..658
                     /locus_tag="VLA60_07090"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSF09161.1"
                     /translation="RLNRSWFSPGESNIYGSILFRQDKPIHQEYLGWIPLMAGVAIAQ
                     AIETHTGIRIHLKWPNDLLIEDRKVGGVLSESFSNAAKNISVIIGFGINVNLSESELP
                     QDLRNSATSLQIHCHQPVDRESLIIKIITALERGWEDLNTKGHQTWQLEYKTRCITIG
                     QTVQVQFPDGNQLYGHAHSVGEGGQLRIIPSPSTTNDQSARMRDIHSGEILHIRTAHL
                     "
     gene            692..1483
                     /locus_tag="VLA60_07095"
     CDS             692..1483
                     /locus_tag="VLA60_07095"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSF09162.1"
                     /translation="MYFFFMLLAIDIGNSHIVCGVFKEGALISHWRLSTDQSKTPDEY
                     NIILRSLFEFNNVNPKDILGCIVSSVVPPLTHVFDMLAHSLLGQQPLVVTSACPHGLT
                     LRYDNPEEIGTDRLVNAAAAFAHYRQYLIIVDFGTATTFCIITNQGEYLGGAIAPGLK
                     NAADTLHLKTAKLPKVDLAIPESIIGRDTATSIQAGIMYGYAGLVDEIVRKIQQEIGQ
                     SALVIATGGLAPTIVPISHTIQEVRPHLTLEGLSLLYGRMTSSCG"
     gene            1674..>2223
                     /gene="grpE"
                     /locus_tag="VLA60_07100"
     CDS             1674..>2223
                     /gene="grpE"
                     /locus_tag="VLA60_07100"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSF09163.1"
                     /translation="MAKSVSCSDDTQEHSQNDLQTKAPVNGGATQEDLQLLQEKYLRL
                     AAEFENYKRRAQRDQSDSIRFGNESLLKNLLPIIDNLERAIQFAKDAGTSGALLEGVE
                     LTHKQFLETVGKLGVRQISSQGSSFDPAVHQAVVQVESENAKPNTVVEEFQKGYFLHD
                     RILRPAMVSVAKEKSDLTDLSSS"
BASE COUNT          632 a          512 c          486 g          593 t
ORIGIN      
        1 tcggttgaat cgatcctggt tttcccctgg agagtcaaat atttacggat cgattctttt
       61 cagacaagat aaacccattc accaagagta tttaggatgg atccccttaa tggccggagt
      121 ggctattgcc caggcaattg aaacacacac aggaattcgc attcacctca agtggccgaa
      181 tgatttactg atagaagacc gtaaggtggg aggcgtatta tccgaatcct ttagcaacgc
      241 agcaaagaat atatccgtga ttatcgggtt cgggatcaac gttaacctct cggaatcaga
      301 acttccccaa gatcttcgaa atagtgccac ttccttgcaa attcactgtc atcaaccagt
      361 ggatagggaa tctctcatca taaaaatcat cacagccctg gaaagaggat gggaagattt
      421 gaataccaaa gggcatcaaa cttggcaatt agaatataaa actcggtgta tcaccattgg
      481 acagacggtt caggttcaat ttcctgatgg gaatcaactg tatggccatg cccattccgt
      541 aggagaaggt ggccaacttc ggatcattcc atccccttcc accacaaatg atcaatcagc
      601 taggatgcgg gatattcatt cgggagagat ccttcatatt cgaactgcac atctttaata
      661 gaacccattc tcaaggatag cctcaactta gttgtatttt ttctttatgc ttctggcaat
      721 cgacattggg aactcacata tcgtctgtgg tgtattcaag gaaggcgctc tcatatcgca
      781 ctggcgttta tctactgacc agtccaaaac ccccgatgaa tacaacatca ttcttcgctc
      841 cctgtttgaa ttcaataacg tgaatcccaa ggacattctg ggttgtatcg tcagtagcgt
      901 cgtcccccct cttacccatg tatttgacat gttggctcac tctttactcg gccaacaacc
      961 acttgtcgtc acaagtgcct gtcctcatgg actcacactg cgatatgata acccggagga
     1021 aattggaaca gatcgtctcg tgaatgcagc tgcggctttt gcccattatc gacaatatct
     1081 tattattgtt gattttggca cggcgaccac attttgtatt attaccaacc agggggaata
     1141 tctgggtgga gccattgccc caggtttaaa aaatgcggca gataccctcc atttaaaaac
     1201 ggcaaaactc cccaaagtcg atctggcgat cccggaatct atcattggca gggacacggc
     1261 cacgagcatc caggcaggga ttatgtacgg gtatgctgga ttggtggacg aaatcgtcag
     1321 aaaaattcag caggaaattg gacagtcagc gttggtcatt gcgacggggg gacttgctcc
     1381 aaccatcgtt ccaatttccc acacaattca ggaggttagg ccccatctca cattagaagg
     1441 attatccctt ctctacggac ggatgacttc ctcctgtgga taatttttcc tctatttttc
     1501 ttctttgctc tttttaatca aaaaacatat gaaatgtaag aggatgggtt gacttgtttg
     1561 aggaagtgac tatctattct cggtagcatt gtgaaatgtc actatcttca tattggtcat
     1621 tcatgactca acaaccggat aaaaacaaac ctatgccgac gactgaggag agtatggcaa
     1681 aatccgtttc atgttcagac gacacacaag aacattcgca aaatgacctt cagacgaagg
     1741 cgccggttaa tggcggagca acgcaggaag accttcaatt gctccaagag aaatatttac
     1801 gacttgctgc ggaatttgaa aattataaac gccgggcaca gcgagaccaa agcgattcta
     1861 ttcgattcgg caatgagtcc ctgctaaaaa atttgcttcc cattattgac aaccttgagc
     1921 gagctatcca gtttgcaaag gatgctggaa ccagtggcgc tttgctggaa ggtgtggaac
     1981 tcactcacaa acaatttctg gaaactgtcg gtaagttagg cgttcggcaa atctccagtc
     2041 aaggaagttc atttgatccg gctgttcatc aagcggtggt acaggtggaa tcagaaaatg
     2101 caaaaccgaa caccgtcgtc gaagaatttc aaaaagggta ttttttgcac gaccgtattt
     2161 tacgaccggc tatggtaagt gtggcaaaag aaaaatcaga tctgacagat ctctcttctt
     2221 cag
//
LOCUS       DATIAL010000229         8581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_149201, whole genome shotgun sequence.
ACCESSION   DATIAL010000229 DATIAL010000000
VERSION     DATIAL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149201"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..577
                     /gene="fabZ"
                     /locus_tag="VLA60_07105"
     CDS             122..577
                     /gene="fabZ"
                     /locus_tag="VLA60_07105"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HSF09164.1"
                     /translation="MAESELDILQIQALLPHRYPFLLVDRILEFQDGTRIVGIKNVTA
                     NEQFFQGHFPGHPIMPGVLLLEVMAQVGGVLARKTAMGTGRPTVFLTGIEKAKFRRPV
                     VPGDQLRVEVEVIRRKDPFWKMAGKILVESSLVCEAEMTAMVKVEGSEA"
     gene            649..1458
                     /gene="lpxA"
                     /locus_tag="VLA60_07110"
     CDS             649..1458
                     /gene="lpxA"
                     /locus_tag="VLA60_07110"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HSF09165.1"
                     /translation="MNIHPTAIVHPKATVGEDVVIGPYCVVGEHVTIGDQTELVAHVC
                     IEGHTDIGRRCRFFPFVSIGTPPQHLQYKDEPTRVCIGDHNIVRENVTINRGTAFGSG
                     VTRIGHHNFFMAYTHVAHDCELGNYIVMANAANLAGHVSVGNSVVIGGLVGVHQYVRI
                     GEYAMVGGCSALGRDVPPFVRAAGGYRAQMFGLNTIALRRHGFSGARMNTLKAAFELL
                     FRQGHRLQEAIKLARNEYGESTDVMTLLTFLESTSRGICQVSSRELDDEEE"
     gene            1486..2355
                     /gene="lpxI"
                     /locus_tag="VLA60_07115"
     CDS             1486..2355
                     /gene="lpxI"
                     /locus_tag="VLA60_07115"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249556.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HSF09166.1"
                     /translation="MISSEQKERIGLIAGNGRFPIIFADNVRRLGFSVSAIAHVGETL
                     PELESHVDRIYWLKVGQFSKALAALKGDGIRQAVMLGGIHKTHVFTALRPDLRALAIF
                     SRLKHWKDDAILRAVAAELEREGIQIRESTFGLEGILAREGNLAYRKPTSKEWDDIQF
                     GWDTLETLGKLDIGQCVVVKNRVIVAVEAVEGTDEAITRGGTLGGKGTVAVKRTKPHQ
                     DLRFDLPAVGPQTIQSMVAVKASVLAIEAGRTVVLDREEMCANARSAGIAIVGIAPTG
                     LVQHAASSDAPPR"
     gene            2374..3399
                     /locus_tag="VLA60_07120"
     CDS             2374..3399
                     /locus_tag="VLA60_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSF09167.1"
                     /translation="MVFRVVSLEPLSLFSYSATTDPYMTQSLRVGVIGVGHLGRHHAR
                     IYGTLPSVTLVGVSDTDTSRGHLVADECGVAYFQDLDELLSLVDAVSVAVPTSAHGVV
                     VTTCLQRGCHVLVEKPIASHVWEGEQLVELAKVRGTILQVGHIERFNPIVEVMRPLVS
                     QPGFIECHRLSPFQPRGTDVDVVRDLMIHDLDLLLSLGLGPILQVEARGMPVFTDLPD
                     IANVRILFESGCLANLTASRISTGRLRKIRVYQRDRYLSVDFGGKEAVMNTRRSLEGG
                     TFEVETQHIKGDEGDALTRELGCFIQSILGNPSARGVSGEEGVEALRVATQVVSLIQQ
                     HTGHSPR"
     gene            3448..4578
                     /gene="lpxB"
                     /locus_tag="VLA60_07125"
     CDS             3448..4578
                     /gene="lpxB"
                     /locus_tag="VLA60_07125"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HSF09168.1"
                     /translation="MPKIFLVTGETSGDIHGAHLALALRELNPAVELIGVGGSRMLAA
                     GVSLLPHVKRVDAMGVPGIRQLIQGWKTLRTLTGYLQHERFDVIILIDSPGLNLRLAK
                     AIAQRSCKMIYYIAPQVWAWGSRRLTLIRREISHVLAILPFEEAYFQKAGIPCTYVGH
                     PLLDELKPAYDMVHERQELGLKREDLVIGLLPGSRVREVREVLPAFLSSVEQIRIQYP
                     RVQVLLAQAHSLSDSMVNELLGHFRQQVKVVKGLSNEVMAASNLLLVTSGTATLQAAL
                     IGTPMVVVYRTSPLTYHIAKRLVKIPYISLVNILAGQEIVPELIQDRMTPDCIAHEAL
                     DILQDVRRQHDMTQAFQAIRTALGGAGASKRAAAYILAEATV"
     gene            4575..6329
                     /gene="msbA"
                     /locus_tag="VLA60_07130"
     CDS             4575..6329
                     /gene="msbA"
                     /locus_tag="VLA60_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HSF09169.1"
                     /translation="MKTFWRIVSYLREYRLRLLGAFVCSAGVAGLSAVYAWLVQPVLD
                     GIFIERNQEMLLILPLAVLGVAILKASFAYGQGYLMSYVGHWLVSDIRQQLFIHIVRL
                     PIRFHDANTSGRLMARVISDVNEMANAIPSVLKDIFQQGLTFVAMLSVVFYQNWKLAT
                     IVLVVLPFSSLVLVRVGRRIRKLSKRGQESIGKMASVLKEAFSGIKIVKAYGQEEKEA
                     DRFSLTNQAFRTAKVKSSQTSAMASPLLEVIGVCGVAFIIWYGGGLVIAGEMKPGEFF
                     SFLAAMFMAYAPLRKMSGANESVQRALAGAQRVFQVLDLESELAKDEGKKILQPMAKN
                     LEFSGVTFKYEGSEEAALHEINFTINVGEVVAFVGASGSGKTTLVSLVPRFYRPTEGA
                     VKIDGEDIRLVDRSSLRRQIGIVSQETVLFDDTIRNNIAYGRLDASEEAVIEAARAAF
                     AWEFIERLPQGLDTLVGENGLRLSGGQRQRLAIARAILRDPPILILDEATSALDSESE
                     KLVQKALANLVKARTTLIIAHRLSTVQHADRIVVMNRGKIEEIGTHATLLQQGGLYTR
                     LYQTQFLLTQPEPLPTTS"
     gene            6349..6864
                     /locus_tag="VLA60_07135"
     CDS             6349..6864
                     /locus_tag="VLA60_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HSF09170.1"
                     /translation="MLNWIKLSVAPPLAARMIRLLGHTMRISTIGGEAVDELYRQGRQ
                     IIIAFWHGRQLMMPLAYRGQQASILISQHRDGEIIARIMGYFGFQSIRGSSTRGALRA
                     TRQLLKAGRNGGDLVVTPDGPKGPACTVQSGVIYLAKATGLPIIPLTFACSKKKSLRV
                     GIVSRFPIPVG"
     gene            6867..7073
                     /locus_tag="VLA60_07140"
     CDS             6867..7073
                     /locus_tag="VLA60_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09171.1"
                     /translation="MFVWGPPIWVDQDLSNEQLSGPGLKLEGSLNRLTAQAELAVQDS
                     DPVESFLRRMSSQNLIDMNETFDA"
     gene            7054..8442
                     /locus_tag="VLA60_07145"
     CDS             7054..8442
                     /locus_tag="VLA60_07145"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HSF09172.1"
                     /translation="MRPSTRDQLMYYVMYNVLLVLAFPIVIGLLLTKKRSQRGLWCRL
                     GAVPVELRNLQKPVIWIHAVSLGEVATIVPLLQAMKERYPQWPLVVSTVTETGREVVI
                     KRLDGVAVHCYAPVDVWWAVNRYIRILQPRLFILVESEFWPNLLKNLQRHRVPICLVN
                     GRISSRSFARYCWVRGMMKQVLSCLDLALMQSEHDAERIGRLGAVPNAIHVTGNMKFD
                     QGLEQGQSADAPVSFRALFTCQAAELLIVAGSTHPQEEECLLDAYRQVIAQHPQAVLV
                     MAPRHIERVAKLEQVIQQYGFPCVRRSRLGQNIGDRAVSRGPRVIVLDTRGELAFVYR
                     EAWVTFVGGTLVPVGGHNLLEPAQWGRPVLFGPHVDHCRDIAGQLLGAGGGLQIQNQE
                     DLASQLLRLIAHPSAVEEMGQRALEMVRTHRGVVTRNLQWINQLLDKDNSAFFSLSAK
                     GSSSRKSQEVLR"
BASE COUNT         1867 a         1983 c         2521 g         2210 t
ORIGIN      
        1 tactgaggtt attcaggcgc tggatcggga gagcggagag attcgtatac cctagtcaaa
       61 gggtggttca tggtcataag gaccagtggt gtcagagtaa caaagagaga aaggggtcag
      121 tatggctgag agcgagttag atattctcca gattcaggca ttactgccgc acaggtatcc
      181 tttcctgtta gtggatcgga ttcttgaatt ccaagatggg acgcggattg tgggcattaa
      241 aaacgtcacg gccaacgaac agttttttca agggcatttc cccgggcacc ccattatgcc
      301 tggtgtgtta cttctagaag tgatggcaca agtgggcggg gtattggcca gaaaaacggc
      361 gatggggaca gggcggccca ccgtttttct caccggaatt gaaaaagcca agtttcgtcg
      421 accggtcgtt cccggcgatc aattgcgggt tgaggtcgag gtgatccgga gaaaagatcc
      481 attttggaaa atggccggga agatcttggt cgagtcctcc ttagtctgcg aagcggaaat
      541 gaccgccatg gtcaaagtcg aaggaagtga ggcctagctg caggcaacag ggttgttgcg
      601 cgaagtctgg ctttgaattc attttcaacg caataaagtg agaatttgat gaacattcat
      661 cccacagcaa ttgtccatcc caaagccaca gtaggggaag atgtcgtcat cgggccctat
      721 tgcgtcgtgg gtgaacatgt cacgattgga gatcaaaccg aattagtggc tcatgtgtgt
      781 atagaggggc atacggacat cggccggcgt tgccggtttt ttccgtttgt gtcgattgga
      841 acccctccgc agcatttgca atataaagat gagcccaccc gagtctgtat cggggatcat
      901 aatattgttc gtgagaacgt gacgattaat cgagggacag cttttggaag tggagtgacc
      961 agaatcgggc accataattt tttcatggcc tatacccatg tggctcatga ttgcgagctg
     1021 ggtaattata ttgtgatggc gaatgcggca aacttagccg gccatgtctc agtgggaaat
     1081 tctgtcgtga tcggaggctt agtcggtgtg caccaatatg tacggattgg tgaatatgcg
     1141 atggtgggag gctgttccgc cctaggacgg gatgtcccgc cgtttgttcg tgcagcagga
     1201 ggctatcgtg cacaaatgtt tgggctgaat acgatcgcct tgcgacgcca tgggttttcc
     1261 ggagcacgca tgaatacctt aaaggccgca tttgaactct tattccgaca gggacatcgt
     1321 ctacaagaag ccattaaact tgctcgtaat gagtacgggg aaagcacaga cgtcatgacg
     1381 ttactcacct ttttagaaag taccagtcgt gggatctgcc aagtgtcctc tcgggagttg
     1441 gatgatgagg aggaataagg ctgcggatga cttggtgcgc agggtatgat ttcttctgaa
     1501 caaaaggagc ggattggact gattgcgggt aacgggcgtt ttccgattat ttttgcggat
     1561 aatgtccggc ggttagggtt ttcggtttcc gccattgcac atgtgggtga gacccttccg
     1621 gaacttgaat cccatgttga tcgcatttat tggctcaagg tcgggcagtt tagtaaggct
     1681 ttggcggcat taaagggtga tggtatccgc caggccgtca tgttgggtgg catccacaaa
     1741 acgcatgtct ttacggcact tcggccggac ctcagggcct tggcgatttt tagccgtttg
     1801 aaacactgga aggacgatgc catcctcaga gcggtggccg cagaattaga gcgggaaggg
     1861 attcagattc gtgagtccac gtttggcctt gaaggtattc ttgcgaggga aggcaatctc
     1921 gcctacagga aaccaacgag caaagagtgg gacgatattc aatttggttg ggataccctg
     1981 gaaaccctgg gaaagttaga tattgggcaa tgtgtcgtgg tgaaaaatcg ggtgattgtc
     2041 gccgttgaag ccgttgaggg aacggatgaa gccattactc gtggagggac acttggtggc
     2101 aagggaaccg tggcggtcaa acgcacgaaa ccgcaccagg atcttcgatt tgatttgccg
     2161 gctgttggtc ctcaaaccat tcaatccatg gtcgcggtta aggcttcagt tctcgcgatt
     2221 gaagccgggc ggaccgttgt tcttgatcgt gaagagatgt gtgcgaacgc cagatcagca
     2281 ggtatcgcga tcgtgggaat cgctcccaca ggccttgtac agcatgccgc gtcttctgat
     2341 gctccaccac ggtaagggga cgggttcccc atcgtggtgt ttcgtgtggt gtcactggaa
     2401 ccgctgtctc tcttttccta ttcagcgacg accgatccct acatgacaca atccttacga
     2461 gttggggtga ttggtgttgg tcatttgggc cgacatcatg ctcgcattta tggaaccctg
     2521 ccttccgtga ccttagtggg ggtctctgat accgatacct cacgcgggca tctggtggcg
     2581 gatgaatgtg gagtagcata tttccaggat ttggacgagt tgctgtcatt ggtcgacgcg
     2641 gtgagtgtgg cggtcccgac ctcggcgcat ggtgtggtgg tcacgacctg tttgcaacgc
     2701 gggtgccatg ttctcgtgga aaagcccatt gccagccatg tgtgggaagg tgagcagtta
     2761 gttgaactgg cgaaagtccg ggggaccata ctgcaggttg gacatattga acggttcaat
     2821 ccgatcgtag aagtgatgcg tcccctcgtg agtcagcccg gctttattga atgtcaccgt
     2881 ctaagtcctt ttcagcctcg agggaccgat gtcgatgtgg tacgggatct catgattcat
     2941 gatttggatc tgttgttatc cttaggtttg ggccctatcc tgcaggtgga agcgagagga
     3001 atgccggtgt ttaccgatct gcccgatatt gccaatgtgc gaattctctt tgaaagcggg
     3061 tgtttggcga atttgactgc aagccgcatc tcgactggac gactgcgaaa gatcagagta
     3121 tatcaacggg atcgctatct ctcggttgat tttggtggaa aagaggctgt gatgaacacc
     3181 aggaggagtt tggaaggtgg aacttttgag gttgaaacgc aacatatcaa gggtgatgaa
     3241 ggcgatgccc tgactcgtga attgggctgc ttcattcaat cgattctggg gaatccttcc
     3301 gcccgaggtg tctcaggaga agaaggggtg gaagccctgc gagttgcaac ccaagtggtc
     3361 tcgctcattc aacaacatac cgggcactcg ccacggtaat ccgcgtcagg gcgggctatt
     3421 tgccatgagc ggtctgttag agtcccgatg ccaaaaattt tcttagtgac cggtgaaaca
     3481 tctggcgata ttcacggagc gcacttagct ctggcacttc gggaactgaa tcccgctgtg
     3541 gaattaatcg gtgtgggtgg cagtcggatg ttggcagccg gggtctcact tcttccgcat
     3601 gtcaaacgtg tcgatgccat gggcgttccg gggatccggc agctcataca ggggtggaag
     3661 accctacgaa cgttaaccgg ttacctccaa catgaacggt ttgatgtcat tatcctcatc
     3721 gacagcccgg gattgaatct tcgcctggcc aaggccatcg cacagcggtc atgtaaaatg
     3781 atctattaca ttgcccctca ggtgtgggca tggggaagcc gtcgtttaac actcattcga
     3841 agagagatca gccatgtgtt agcgattctt ccattcgaag aggcctattt ccaaaaagcc
     3901 ggcattccct gcacatatgt tggccatcca ttattggatg agctcaagcc tgcctatgat
     3961 atggtgcatg agcgtcagga attaggattg aaaagagagg atctggtgat cggtctgctg
     4021 cctgggagtc gtgtccggga agtccgggag gtgttgccgg ccttcttgag ttccgttgaa
     4081 cagattcgga tccaataccc tcgagttcag gtccttcttg cccaagcgca ttcactgtcc
     4141 gattcgatgg tgaatgagct ccttggacat ttcaggcaac aggtcaaggt ggtcaaaggt
     4201 ctatccaacg aggtgatggc ggcctcaaat cttttattgg tgacctctgg gacggcgacg
     4261 ttgcaagcgg ccctgattgg aacacctatg gtcgtggtct atcgaacatc tcccttgacc
     4321 tatcatattg ccaaacgtct ggttaaaatt ccttatatta gtctcgtgaa tattttagcc
     4381 ggtcaagaaa ttgttccgga gctgattcag gatcgtatga ccccggactg tattgcgcat
     4441 gaagccttag acatcttgca agacgttcgt cgccaacacg acatgaccca ggctttccag
     4501 gccattcgga ctgctctggg tggggcggga gcgtcgaaac gggctgcagc ctatatcctt
     4561 gctgaggcga ccgtatgaag accttctggc gaatcgtgtc gtatctgagg gagtaccgcc
     4621 tgcggttact tggcgcgttt gtctgttccg ccggggtggc aggattatca gcggtctatg
     4681 cgtggcttgt ccagccggtt ctggatggaa tttttattga gcgcaatcag gagatgttgc
     4741 tcatccttcc gttggctgtc ctgggtgtcg cgattttaaa agcctccttt gcctatggac
     4801 agggttatct catgagttat gtgggccact ggctggtgtc cgatattcgc caacaattgt
     4861 ttatccatat cgtgcgcctg cctattcgtt ttcacgatgc aaatacgtca ggacggttga
     4921 tggccagggt gattagcgat gtcaacgaaa tggcgaatgc cattcccagt gtattgaagg
     4981 atatctttca acaggggctc acgtttgtgg cgatgctgtc cgtggtcttt tatcaaaatt
     5041 ggaaactcgc gacgattgtg ttggttgtat tacccttctc ctcattggtg ttggtccggg
     5101 tcggccgacg gattcgtaaa ctgtcaaagc gtggccagga atccattgga aaaatggctt
     5161 ccgtcttaaa ggaagcgttt tccggtatca agatcgtgaa ggcctatgga caggaagaga
     5221 aggaggctga ccgattctcg ttgacgaatc aagcctttcg gacagcaaaa gtgaagtcgt
     5281 ctcaaacctc agcgatggcc tctccacttc ttgaagtcat tggagtctgc ggtgtggcct
     5341 ttattatctg gtatggggga ggattagtca ttgccggaga gatgaagccg ggagagttct
     5401 tttcctttct ggctgccatg ttcatggcgt atgcccccct ccggaaaatg tccggggcca
     5461 atgagtcggt tcagcgtgcg cttgcggggg ctcagcgggt gtttcaagtt ctcgacctgg
     5521 aaagtgaatt agcgaaagac gaaggcaaga aaattcttca acccatggcg aaaaatttgg
     5581 aattttccgg tgtcaccttc aagtatgagg gctctgaaga ggcggccttg cacgagatca
     5641 atttcacgat caacgtgggg gaggtggtgg ccttcgtcgg ggccagtggt agtggaaaaa
     5701 ccacgctcgt gagtctggtt ccacggtttt atcgcccaac ggaaggagct gtgaagattg
     5761 atggtgagga tattcgcctg gttgatcgat catctctgag acggcagatt ggcatagtgt
     5821 ctcaagaaac cgtgttattt gatgatacga tcagaaataa tatcgcctat ggccgtctgg
     5881 atgcgagtga ggaggctgtc attgaggcgg ctcgtgccgc ctttgcctgg gaatttattg
     5941 agcggctccc tcagggactg gatacgctgg ttggggagaa tggactacga ttatcgggtg
     6001 gacaacgtca acgcctcgcg attgccaggg caattttgcg ggatcccccg attctgattc
     6061 tggacgaggc gacgtccgcc ctggattcgg aatctgaaaa gctggtgcag aaagctctgg
     6121 caaatctggt aaaagcccga acgaccttga tcattgccca ccgattgtcc acggttcaac
     6181 atgccgatcg gatcgtggtg atgaatcgcg gaaaaattga agaaatcggc actcatgcca
     6241 ccttgcttca gcagggaggc ctctatactc gattgtatca aacgcaattt ttactgacgc
     6301 aacccgaacc gttaccgacg acctcctaaa ttgaagagcg ccgttcctat gttgaattgg
     6361 atcaaactgt ccgtcgctcc gccattggcc gcacgcatga ttcgcctttt gggacatacg
     6421 atgcgtatat ccaccatcgg tggggaagcc gtggatgagt tgtatcgcca aggccgacag
     6481 atcattattg ccttttggca tggccgtcag ttgatgatgc cgttggccta ccgtgggcaa
     6541 caggcctcaa ttttgattag ccagcatcgg gatggagaaa tcatcgcgcg aatcatgggg
     6601 tattttggat ttcagtccat tcgcggctcg agcacgcgcg gggccctgcg agcgaccagg
     6661 caattgttga aggccggtcg gaatggcggt gacctggttg tgacgcccga tggcccgaag
     6721 ggaccagcct gcacggtaca atcgggagtg atttacctgg ccaaagcgac gggattgccc
     6781 ataatccctc tcacctttgc ctgctcaaaa aaaaagtctt tacgagttgg gatcgtttcc
     6841 agattcccta tcccggtggg gtagggttgt ttgtatgggg tccacccatc tgggtagacc
     6901 aggatctttc caacgagcag ctgtccggtc caggcttaaa gctggaaggt tcgttgaacc
     6961 ggctcacggc tcaagcggaa ttagctgttc aggattccga cccggttgaa tcatttctcc
     7021 gccgaatgtc ttcccagaat ctgatcgata tgaatgagac cttcgacgcg tgaccaactc
     7081 atgtattatg tgatgtacaa tgtgctgctg gttttggcct ttcctattgt catcggactt
     7141 ctgctgacaa aaaaacgcag tcaacgcggc ctttggtgcc ggcttggggc ggttccggtc
     7201 gaattgcgga atctgcaaaa accggtcatc tggattcatg ccgtctcctt gggcgaggtg
     7261 gcgacgatcg ttccgctgct ccaggcgatg aaggagcggt atcctcagtg gccgttggtc
     7321 gtgtccacgg tgacggagac cggtcgggaa gtcgtgatca agcgtttgga tggtgtggct
     7381 gtccattgtt atgctcccgt tgatgtttgg tgggcggtta accggtatat ccgcatactt
     7441 cagccccggc tctttatttt ggtcgaatca gaattctggc ccaatctgct taaaaatctt
     7501 caacggcatc gggtgcccat ctgtcttgtg aacgggagaa tttcttcacg atcctttgcc
     7561 agatattgct gggttcgagg catgatgaag caggtgttga gctgcctcga tcttgccttg
     7621 atgcaatcgg aacatgatgc tgagcgcatc ggccggttag gggctgtgcc aaatgccatt
     7681 catgtcaccg ggaatatgaa gtttgatcag gggcttgagc aaggacagtc tgccgatgcc
     7741 cctgtctctt tcagggcact ctttacgtgc caggctgctg agctcctcat tgtggctggg
     7801 agtacgcacc ctcaggagga agagtgtttg ttggatgcgt accggcaggt gattgcccaa
     7861 caccctcagg cggtactggt gatggctcca cgacatatcg agcgtgtggc taagcttgaa
     7921 caggttattc aacagtatgg attcccttgt gtccgtcgca gccggttggg acaaaatatc
     7981 ggagatcggg ctgtttcccg tggccctcgc gtcatcgtgt tagacaccag gggggagttg
     8041 gcctttgtct accgggaggc ctgggtcacg tttgtggggg ggacactggt tccggttggc
     8101 ggacataatt tgttggaacc tgcacaatgg ggtcgccccg ttctatttgg tccccatgtc
     8161 gatcattgcc gggatattgc cggacagctt ctgggagcag gaggaggcct acaaattcag
     8221 aatcaggagg atttggcctc tcaattgctt cgtctgatcg cgcatccttc agcagtcgag
     8281 gagatgggcc aacgggcgtt ggagatggtc cggacccatc ggggcgttgt caccaggaat
     8341 ctgcaatgga ttaatcaatt gttggacaag gacaattcgg cctttttctc tttgtcggcg
     8401 aaggggtctt cttcccgcaa atctcaagaa gtgctccgat aagcttccat tagagccctg
     8461 aataagggga gaatatgatc tccgccatgt gtccttcgtg aacacgacgt ctccttcgct
     8521 tggtgtgaac ccggaacggc tctggcgttg gttggaccgg cgttgcccat ggattctccg
     8581 g
//
LOCUS       DATIAL010000230         4375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1332466, whole genome shotgun sequence.
ACCESSION   DATIAL010000230 DATIAL010000000
VERSION     DATIAL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332466"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VLA60_07150"
     CDS             complement(<1..288)
                     /locus_tag="VLA60_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09173.1"
                     /translation="MCPLLKKIFHYVLLVVVVSVLLACDKVSIPRLTYEDKHEPRIPV
                     TITYAFAPNLLTYTQTVDGCGLPYTIPVGEIISKTFLQVGQEQFNGVRAEPP"
     gene            636..1406
                     /locus_tag="VLA60_07155"
     CDS             636..1406
                     /locus_tag="VLA60_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09174.1"
                     /translation="MWRHIWLIVGFLSAFSFFGCSLDNTPKRLPAYLPQPSNLAGGDG
                     ATIALPGEGVTAVLVVLNDSGFEKAAPELRRGTLENLGEHLKTELQKQLPIQIHSVVY
                     PDDLKPKGSVDPFIQLGNEQNVPYLVLAVLSSTEYEVFDRFPMGGLQQPTGMRGGGMP
                     GYRAENYARLELALLDARTGQPVVSTDGQAWATLERLVVPLESNVYPVVRRAQTQPPI
                     YPNSEEEEYETLRWVSGKDAIAQAVMHLEILWKKNQAT"
     gene            1411..2163
                     /locus_tag="VLA60_07160"
     CDS             1411..2163
                     /locus_tag="VLA60_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09175.1"
                     /translation="MTLSKLLCLCVLAFTLLGCGAGPTPRLIHLVGINASDYMGPPPE
                     KGEWMGAPNLGLVVHADDTAQNSAPAIISKYLETLTRRTETFLRQRCAFQDIVTVPPF
                     SPAVNFSQELKVQGQSLHVPYVIVVVLSSREKTGPVKIGEATMMTQMNGTGIENSALA
                     EVGILRLSDFQLVYLTQGRGFESLEQLDVPIGKNPPSAIEARDLLRAGAGQQALDRAL
                     EHVGTACQPVAKPRLAVTGSFQIADRVRSPVL"
     gene            complement(2114..2611)
                     /locus_tag="VLA60_07165"
     CDS             complement(2114..2611)
                     /locus_tag="VLA60_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HSF09176.1"
                     /translation="MADNYSFDVISRIDMQEMKNVVDQTEKEVGQRFDFKGSKTELTL
                     KEKDKQLVVISDDDYKLNAVLDILKAKCVKRNVSLKGLTYGKVEEALGGTVRQTITIQ
                     SGLPEDKAKAITKSIKEAKFKAQGQIQGEQVRVQSKSKDELQAVMKHLKEQDFGLDLQ
                     FENYR"
     gene            complement(2648..>4375)
                     /locus_tag="VLA60_07170"
     CDS             complement(2648..>4375)
                     /locus_tag="VLA60_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HSF09177.1"
                     /translation="LEDGTNFYPANKYILFGHHFAAIAGAGPLLGPVLAAQFGFLPGF
                     LWIVIGAVLAGAVQDFIILVASMRRNGRSLPEIAHAELGPITGMATAIAVLFIVVVAL
                     AGLGLAVVNALYHNAWGTFTIVMTIPIALLMGLYLQKLRQGQVGEITLIGLGLLIVAI
                     LVGRTVAQSEWAEWFLWDRTTLTWSLAAYGFLASVLPVWMLLVPRDYLSTFMKLGVIA
                     LLAVGVIVLAPTIEMPRTTIFTAGNGPIIPGTLFPFLFITIACGAISGFHSLVSSGTT
                     PKLISRESQAIVGYGAMLLESFVGVVALIAACLLVPGDYFAINTSLPADTLQTMGFPT
                     AHITELSTLVEVDVSGRPGGAVSLAVGMASIFSSLPGMSGLMAYWYQFALLFEALFIL
                     TTIDAGTRVARYLVQELGGRFYTPLKQINWWPGELGASLFVVGGWGYLIGTGSIATIW
                     PMFGAANQLLGMLALCIATTVLIKMNKTAYLWVTVIPMVFVGVITLAGCYELLVIFIS
                     RALSTEGSQSLTMAINASLVGLVAILALIVLTDSARKWYGYLIHKQPLNSTEVIEGEG
                     IQLPAGRCC"
BASE COUNT         1046 a         1074 c         1183 g         1072 t
ORIGIN      
        1 gggaggttcg gctctcacgc cattaaattg ttcttggccg acctggagaa atgttttgga
       61 tatgatctcg cctactggaa tagtatatgg caacccacac ccatccacgg tctgagtata
      121 agtcagaaga ttgggtgcaa aggcgtaggt gatcgtgaca gggattcttg gttcgtgttt
      181 atcttcgtaa gtcaggcggg gaatggagac tttgtcgcag gcgagaagaa cggacacgac
      241 aaccacgagt aagacgtaat ggaaaatttt tttaagcagt gggcacaccg ccttacccta
      301 ccccaagatc ctgaaagatg gcaactggat cccgtgtttt tccagagact ttcctcttgc
      361 ttcggcgggt ccgaatctta ggcgaatatc cggttgccca aacaataatt ggtcttcgag
      421 ggaaagacag tgaaaaccat gaggagccac ggaggaaggt cctgggaagc aatagtatgg
      481 atgatcctga gagggacaat gaatcccggc cttcaacttg tcagcttttg acggatatca
      541 ccttcaccgt ttctcctgtt cttgacgatc cttgagtaaa gggaagagaa tttaacattc
      601 agacggacat ttttttaacc aatggaggct tcattatgtg gcgtcacatc tggttgattg
      661 tcgggttcct ctctgctttt tctttctttg gttgttcgtt ggataacact cccaaacggt
      721 tgccggctta cctgccccaa ccctccaatc tggcaggcgg ggacggtgca acaattgcgc
      781 ttccagggga aggagtgact gccgtcttgg tcgttctgaa tgattcggga tttgaaaaag
      841 cagctcctga acttcgccga gggacgttgg aaaatcttgg ggaacatctg aagacggaac
      901 ttcaaaaaca attaccgatt caaattcatt ccgtcgtgta tccggatgat ttgaaaccca
      961 aggggtccgt ggacccattc atccaattgg ggaatgagca aaacgtgccc tatctggtgt
     1021 tggcggtatt atccagtacc gagtatgagg tctttgatcg ttttccaatg ggcgggttgc
     1081 agcagccgac gggtatgcgg ggtgggggta tgccagggta ccgtgcggaa aattatgcgc
     1141 gtctggaact cgcgttgctg gatgcccgga cagggcaacc ggtagtctcc acggatggtc
     1201 aagcgtgggc gaccctggaa cggctggttg tcccgcttga atctaatgtc tatccggtcg
     1261 tccgacgggc tcagacccaa cctcccattt atcccaattc agaggaggag gagtatgaga
     1321 cgcttcgatg ggtttccggg aaggatgcca ttgcccaggc ggtgatgcac ttagaaatat
     1381 tatggaagaa gaatcaagcg acctgattgg atgactctat caaaactatt gtgcctgtgt
     1441 gtgttggcgt ttactttgtt ggggtgtggg gcaggtccca cgccccgtct gatccatctt
     1501 gtcggaatta atgcctctga ttatatggga cctcctccgg agaaaggtga atggatgggt
     1561 gcgcccaacc ttgggcttgt tgtccatgca gatgataccg ctcagaattc cgctccggcc
     1621 atcatttcca agtatcttga gacgcttact cgacgaaccg aaacgtttct ccggcagcgt
     1681 tgtgcctttc aagatattgt gactgtcccg ccgttctctc cggcggtaaa cttttctcag
     1741 gaactgaaag tccaagggca gagtttacat gtcccctatg taatcgtggt tgttctttcc
     1801 agtcgtgaga aaactggtcc tgtaaaaatt ggagaggcca cgatgatgac tcaaatgaat
     1861 gggaccggta ttgaaaattc agcattggct gaggtgggta ttctgcgtct atcggatttt
     1921 caactggtct atttgacgca ggggcggggg tttgaaagtt tggagcaact ggatgttccc
     1981 attgggaaaa acccgccttc tgctatagag gctcgggacc tgttgagagc tggagccggt
     2041 caacaggctc tggatcgggc cttagaacat gtagggacgg cctgccagcc tgtggcgaaa
     2101 ccgcgcctgg ccgttaccgg tagttttcaa attgcagatc gagtccgaag tcctgttctt
     2161 tgagatgttt catgacggct tgcagttcgt ctttgctttt gctttgcacc ctgacttgtt
     2221 ccccttgaat ttgtccctgg gccttaaatt tggcctcttt aatcgatttc gtgatggctt
     2281 tagccttgtc ttccggaagc ccgctttgaa tggtaatggt ttggcgtacg gttccgccta
     2341 aggcttcttc cactttccca tacgtcaggc ctttcaacga cacatttcgt ttgacgcatt
     2401 tggccttaag gatatccaag accgcgttaa gtttatagtc atcatccgaa atcaccacta
     2461 attgtttgtc cttttctttt agcgtcaatt cggttttgga tcctttgaaa tcaaagcgtt
     2521 gtccgacttc tttttcggtt tgatccacca catttttcat ttcctgcatg tcgatgcgag
     2581 aaatgacgtc gaaggaatag ttgtctgcca taagcctcct ttcaagttgt tgtgacaggc
     2641 ccctggatta gcagcaccgt cctgcgggaa gttgaatgcc ttccccttcg atcacttcgg
     2701 tgctgttcag tggctgtttg tgtataagat agccgtacca ttttctggcg ctgtctgtga
     2761 ggacaatgag tgcgagtatg gccaccaggc cgacgagtga ggcattgata gccatggtca
     2821 ggctttgcga cccctctgtt gagagggccc gactgataaa gatcaccaag agttcatagc
     2881 atccagccag ggtaatcacg cccacaaata ccatcgggat caccgtcacc cacagatagg
     2941 ctgtcttgtt catcttgatg agcacggtcg tggcaataca gagcgcgagc ataccgagta
     3001 attgattggc tgcgccaaac ataggccaga tggtcgcaat ggatcctgtg ccaataagat
     3061 acccccaccc cccaaccacg aacagactgg cgcctaattc gccgggccac caattgatct
     3121 gcttgagcgg ggtatagaat cgtcccccta attcctgaac cagataccgg gcgacccgtg
     3181 tcccagcatc aatcgtggtc aaaatgaaca gggcttcgaa caagagcgcg aattgatacc
     3241 aataagccat gagcccggac atccccggca agcttgaaaa aatcgaggcc atgccaacgg
     3301 ccagggacac cgcccctccc ggacgacccg atacatctac ctcaacaagc gtggagagtt
     3361 ccgtaatgtg ggcggttgga aatcccatcg tttgcagggt gtctgcgggc aggctggtgt
     3421 tgatggcaaa atagtcgccg ggaacgagaa ggcaggcggc gatgagcgcc actactccga
     3481 caaagctttc cagaagcatg gcaccatagc cgacgatggc ctgagattcc cgactaatca
     3541 gcttgggtgt cgttcctgat gagaccaggg aatgaaagcc ggaaatggcc ccacaggcaa
     3601 tggtgataaa gagaaacgga aacaacgttc ccggaatgat cggtccattg cccgccgtaa
     3661 atatggtggt gcgaggcatt tcaatggtgg gcgcgagtac tatgacgccg acggccagaa
     3721 gggcgatcac acccaatttc ataaatgttg agagataatc ccggggcact aataacatcc
     3781 agacggggag aactgaggca agaaatccat atgccgcaag gctccatgtg agcgtggttc
     3841 ggtcccataa gaaccattca gcccattctg attgtgccac ggttcggcca acgagaatgg
     3901 ctacgatcaa gagtcctaag ccgatcagcg taatttcccc gacttgtccc tggcgaagtt
     3961 tttgaaggta tagccccatg agcaaggcga tgggaatggt catgacgatg gtaaaggtgc
     4021 cccaggcatt atgataaagc gcattcacca ccgccaggcc taatccggct agcgcgacca
     4081 ccacgataaa gagcacggca atcgccgtgg ccatcccggt gatgggacct aattcagcat
     4141 gggcgatctc cggaagtgaa cggccgtttc gcctcatgga ggcgacaagg ataataaaat
     4201 cttgaactgc gcctgccaga accgcgccaa tgacaatcca taaaaacccc gggaggaagc
     4261 caaattgtgc cgccaagacg ggaccgagta atgggccggc tccggcgata gccgcaaaat
     4321 gatggccgaa gagaatgtat ttgttggcgg gataaaaatt ggtgccgtcc tccag
//
LOCUS       DATIAL010000231         3599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_365872, whole genome shotgun sequence.
ACCESSION   DATIAL010000231 DATIAL010000000
VERSION     DATIAL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365872"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1687)
                     /gene="cimA"
                     /locus_tag="VLA60_07175"
     CDS             complement(107..1687)
                     /gene="cimA"
                     /locus_tag="VLA60_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HSF09178.1"
                     /translation="MNSKFPVIEIYDTTLRDGAQAEDVSFSVEDKVRIALKLDELGVH
                     FIEGGWPGANPKDIEFFQVMKSTPLQHAKIVAFGSTRKAKNTAKSDPTLEALLSAETD
                     IVTIFGKSWTLHVTEALETTLAINLELIADSISYLRSRGRQVFYDAEHFFDGYKTDPA
                     YAMKTVQVAAEAGAERIVLCDTNGGTMPWDIGEIFAEIRKNCSVPLGIHAHNDAEMGV
                     ANSLIAVQAGAIQVQGTINGIGERCGNANLCSVMANLELKMERTALGDGRLAHLRAIS
                     HYVAEMANLTPNKRQPYVGDTAFAHKGGVHIHAVQKNPLTYEHVNPDIVGNHRRVLIS
                     DSSGRSAVFGKMETFGLDLPQDNPKVLELLDSLKTLEYEGYQFEGAEGSFELLVRKAM
                     GTYTPSFEFLGCRIIVEKRKTNEDPISEATIMVKVGEVIEHTAAVGDGPVNALDHALR
                     KALEHFYPQLKEVKLLDYKVRVLTGRHGTGSRVRVLIESGDHKEKWGTVGVSENIIEA
                     SWQALADSIEYKLMKEKP"
     gene            complement(1729..2964)
                     /locus_tag="VLA60_07180"
     CDS             complement(1729..2964)
                     /locus_tag="VLA60_07180"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HSF09179.1"
                     /translation="MALYIQKFGGTSVGSLERIQNVARLIQHTYREGHQLVVVLSAMS
                     GETDRLMKMAKTLSVDPDDRELDVLLSSGERVTIALMAMTLKSLGIRAQSFTGRQVGI
                     VTDSTHTKARVSRVDTDRVLGALRDGIIPIVAGFQGINAASDVTTLGRGGSDLTAVVL
                     AAALKADTCFIYTDVDGVYTADPNIVPGARRLDKISYEEMLELASLGAKVLQSRSVEL
                     AAKYQVPVEVRSSFQDGPGTCVVKEDVDMEQVLVSGVTGDRNQAKVTVVGVPDHPGIA
                     ATLFCSIADAAINVDMIIQNVSQDSLTDISFTVPRADLPRGMSLIRDIAKSVEARTVE
                     VNESIAKVSLVGVGMRSHSGVAARMFQTLSREGINIMMISTSEIKISCVVEEKYMELA
                     VRALHQEFELEVPPGSRKA"
     gene            complement(2970..>3599)
                     /locus_tag="VLA60_07185"
     CDS             complement(2970..>3599)
                     /locus_tag="VLA60_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09180.1"
                     /translation="SRAILCHHPVNHERLSAGLKPANCLWLWGAGKPVELPHLHERWP
                     VNGLVISPDGPYIGVGMAAGLHTVKVKHVGEGDIAWLQAVANQASTALDRHDLVCLHI
                     PFYEWGLKDGQVVPPSQLVEYLGQVDEQVIGAIHQSVGSTDSIRILVLVTPCPNWQKD
                     GGSSLSPYLVFEGPGEKSENHGGAMNEREVANRPLRDATKLFERFMVTH"
BASE COUNT          836 a         1065 c          828 g          870 t
ORIGIN      
        1 gttgttgctg gcagcaacgt aacgggcaac gcagatgatg tcaagaaccg ggctcttcag
       61 aggattcccc tttgtcagcc tatactcgga gagaccgcta tcacgtttac ggcttctcct
      121 tcatgagctt atactcaata ctgtcggcca gggcctgcca actggcttca atgatgttct
      181 ccgatacccc gaccgtgccc catttttcct tgtgatcccc cgactcaatc aggactcgca
      241 cacgagaacc cgtgccatgt cgcccggtca gcacacggac cttatagtcc agaagtttga
      301 cttcctttaa ctgcggatag aaatgctcca gcgctttccg aagggcatga tcgagcgcat
      361 tcaccggccc atcacccacc gccgcggtat gttcaatcac ttccccgact ttgaccataa
      421 tcgtggcttc ggaaataggg tcttcattcg ttttccgttt ttccacaatg atccggcacc
      481 ccaggaattc gaaggatggg gtataggtcc ccatggcttt ccgcaccaac aattcaaacg
      541 acccttctgc gccttcaaat tggtagcctt catattccaa cgttttgagg gaatccagta
      601 attcaaggac cttgggatta tcctgaggaa gatccagtcc gaaggtttcc atctttccaa
      661 atacggcact tcgtccgctg ctatcggaaa ttaagacgcg gcgatggttg ccgacgatat
      721 cgggattcac atgttcatag gtgagcgggt ttttttgcac cgcatgaata tgcactcccc
      781 ctttatgcgc aaacgcggta tctcccacat aaggctgccg tttattggga gtcagattgg
      841 ccatctcggc cacataatga gaaatggcgc gaaggtgcgc caaacgtcca tcccccagcg
      901 ctgttcgctc catttttaat tccaaattgg ccatcacgga acacaggttg gcattcccgc
      961 accgctctcc aatgccattg atcgttccct ggacctggat cgctcccgct tgcacggcaa
     1021 tcagtgaatt ggcgaccccc atctccgcat cattgtgtgc atggatgcct aacggcacag
     1081 aacagttctt gcggatctcc gcaaagatct ccccgatatc ccatggcatc gttcccccat
     1141 tggtatcaca caacacgatc cgctccgccc cggcttctgc cgcaacttga acggttttca
     1201 tggcgtaggc gggatccgtt ttatagccat cgaaaaaatg ttcggcatca taaaaaacct
     1261 gccgtcccct cgaccggaga taggagatgg aatcggcaat cagttctaaa ttgatggcca
     1321 acgtcgtttc caaggcttcc gtcacatgga gggtccaact tttcccaaag atggtcacaa
     1381 tatccgtttc agcggataag agggcttcca gcgtcggatc ggactttgcc gtatttttgg
     1441 cttttcgcgt cgaaccaaat gccacaattt tggcgtgctg caacggagtc gatttcatga
     1501 cttggaaaaa ctcaatgtcc ttcgggttgg ctcccggcca ccctccttct atgaaatgga
     1561 ccccgagctc atctaattta agggcgatgc gaaccttatc ctccaccgaa aagctgacat
     1621 cttcggcctg agctccatcc cggagggtcg tatcgtagat ttcgatgacc ggaaattttg
     1681 aattcatatc acgcattcgt accatcggtg tcgacacaca tccccacgct aggccttcct
     1741 gctccccgga ggaacttcta attcaaactc ctggtgcaag gcccgaaccg ctaactccat
     1801 atatttttct tccaccacac aagagatttt tatctccgag gtactgatca tcatgatatt
     1861 aatgccttca cgtgagagcg tctgaaacat tctggcggcg acaccggaat gcgaacgcat
     1921 tcccactccc actaacgaaa ctttggcaat ggattcgttc acctcgaccg tccgtgcttc
     1981 cactgatttg gcaatgtctc tgatgaggct catgcctcga ggaaggtcag ctctcggcac
     2041 ggtaaaggaa atatccgtca gggaatcctg actcacattt tgaataatca tatccacatt
     2101 gatggcggcg tcggcaatgg agcagaacaa tgtggccgca atcccagggt gatccgggac
     2161 cccgaccacc gtgaccttgg cttgattccg atcgcccgtg actcctgaga ctaagacttg
     2221 ttccatatcg acatcctctt ttaccacgca ggttccaggc ccgtcttgaa aactggatcg
     2281 cacttccaca ggtacctgat atttcgccgc taactccacc gaacgagact gtaggacctt
     2341 cgcacccaga ctcgctaact ctaacatttc ttcataggag attttatcca atcgcctcgc
     2401 gcctggcaca atattgggat cggccgtgta caccccatcc acatcggtat aaataaagca
     2461 ggtatcggcc ttgagcgccg ccgccaagac cacggccgtc aaatccgagc cgccgcgccc
     2521 taacgtggtg acatcggatg cggcgttgat accttgaaac ccggctacga tgggaataat
     2581 cccgtcacgc aaggctccca gcacccgatc cgtatcgaca cgggagactc gggctttggt
     2641 atgtgtgcta tcggtcacaa tcccaacttg acgtcccgta aaggactgcg ccctgatccc
     2701 aagacttttg agggtcatcg ccatcagcgc aatcgtgacc cgttctccag aagaaaggag
     2761 cacatccagc tcccgatcat ctggatcaac gctcaaggtc ttggccattt tcatgaggcg
     2821 atccgtttcc ccgctcatcg cggacaacac gaccaccaat tgatgcccct cccggtaagt
     2881 gtgttgaata agccgagcca cgttttggat tcgttcaagg ctccccactg atgtaccgcc
     2941 gaatttttga atatacagtg ccataggctt tagtgagtga ccataaatcg ctcaaacagt
     3001 ttagtagcat cacggagtgg gcggttggct acttcacgtt cattcattgc tcccccatga
     3061 ttttcggatt tttcccctgg cccttcaaac accagatagg gtgataggga agaccctcca
     3121 tccttctgcc aattcggaca tggggtcacc aggacgagga tgcggatgga gtcggtgctg
     3181 ccaacagact gatggatggc tccaatgacc tgttcgtcca cttgcccaag atattccact
     3241 aattgtgagg gtggtaccac ctgtccatcc ttcaaacccc actcgtaaaa gggaatgtgc
     3301 agacagacca ggtcatgcct gtccaacgcc gtcgaagcct gattggctac ggcctgcaac
     3361 catgcgatat ctccctctcc tacatgtttg accttgaccg tgtgaagtcc agcggccatt
     3421 ccaacaccga tatacggccc atcgggcgaa atcacgagac cgttgactgg ccaccgctca
     3481 tgtaaatgag gcaattccac aggtttcccg gccccccaca accataaaca attggccggt
     3541 ttcaacccgg cactcaggcg ctcgtgattc acaggatgat ggcagaggat tgccctgga
//
LOCUS       DATIAL010000232         3829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_150535, whole genome shotgun sequence.
ACCESSION   DATIAL010000232 DATIAL010000000
VERSION     DATIAL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150535"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VLA60_07190"
     CDS             complement(<1..766)
                     /locus_tag="VLA60_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HSF09181.1"
                     /translation="MTPSILLFGATSILGFNLARLFPEIIMPFISPGNTSRSVGHWPS
                     LQLENPYWLDCIFAQCKSNILVYCHAVCDVQKCETYPDWAHEINVLQLRRVIEALPET
                     TRLVYVSSDHVFGGDNEYDEHSNLCPISVYGRTRVHAEKLVLERRQSLVIRTGLAIGA
                     SPNGRTGHLDWLRYRTRRNLPITIVEDEYRSVVWVTDLSRRVMKLAHSLETGIRHVSA
                     TRAVSRVELANYLMSFLGKPPNFRRESRHQRTTPHLG"
     gene            complement(898..1467)
                     /locus_tag="VLA60_07195"
     CDS             complement(898..1467)
                     /locus_tag="VLA60_07195"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSF09182.1"
                     /translation="MDKVSDYIYVTNVIPQPICKDVLKEITGKEWKPHNWYSYSTDNF
                     KSEPEKELDTQNTTQALQDTLAPFICQSVEEYMIKFANQEDERIKSFIQTFTPIRFNR
                     YRTGTMMRKHYDHIHSIFDGKYKGIPILSLVGILNEDYEGGEFLFSSKHEIKFKAGDM
                     LVFPSNFMYPHEVKEITKGERYSFVAWAF"
     gene            complement(1652..2314)
                     /locus_tag="VLA60_07200"
     CDS             complement(1652..2314)
                     /locus_tag="VLA60_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSF09183.1"
                     /translation="MKDNFARFNPDRFSFAEDPVLILEDFWKPGERKVFREAMARSKW
                     TALSDMPPVAQAFPNCGNWRKADIDHSEGAHFLQRVGMSCIAAYIESFPNIKGRHVNF
                     NFYSYGAGDCLSTHDDTDDIYSYAQGRRPPIRRLAVATYFHEEWHPDWGGELIIYGSP
                     ASVDSKDSRNSSLHVTYCIPPSPGSLVIFAVPRQHRVCRVDALAGDHERLSIAGWFMT
                     EH"
     gene            2814..3185
                     /locus_tag="VLA60_07205"
     CDS             2814..3185
                     /locus_tag="VLA60_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09184.1"
                     /translation="MGNSILLIDHDRLFRRDVRLYLEKCGFYCKEANDGREALALLDG
                     GFNVDVILSKYQTPIINGLSFLKALSYRVNGQHVRVILLNSHMTNEMEHEAKQAGAFE
                     VLAKPYDLRELLELVARACKQ"
     gene            3285..3671
                     /locus_tag="VLA60_07210"
     CDS             3285..3671
                     /locus_tag="VLA60_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09185.1"
                     /translation="MRPKILLMDDDSELRSTLRDRLIMEGYDVQTAPDGRFGLKLYHE
                     TPVDLVITDLLMPEMDGLEVIRVLCGTPSPPLIIAMSGGGIHDLDFLVEATEFGASRT
                     LSKPFRLDDFVTLVNEILNSTSHPHV"
BASE COUNT         1052 a          880 c          845 g         1052 t
ORIGIN      
        1 tgcccaaatg aggagtcgtc cgttgatgcc ggctctcgcg ccggaagttt ggtggcttcc
       61 ccaaaaagga catcagataa tttgccagct ccactcttga aacggcccta gtggccgaga
      121 catgtcgaat tcctgtctcc aggctatgtg ctaatttcat gacacgtctg gatagatctg
      181 tcacccaaac aaccgagcga tattcatcct ccacaatcgt aataggcagg ttgcgtcgag
      241 tgcggtagcg aagccaatct aaatgacctg tccgaccatt gggcgaagcg ccaatcgcca
      301 gaccagtgcg aattactaaa gactgacgcc gttctaaaac cagcttttcc gcatggacgc
      361 gggtccgacc gtacacactt ataggacaca aattcgagtg ttcatcatat tcattatccc
      421 caccgaacac atgatctgaa gagacataca ccaaccgtgt tgtctcgggc aaggcttcaa
      481 tcacccgacg gagctgtaga acgttgattt catgggccca atcggggtaa gtctcacatt
      541 tttggacatc acacaccgca tggcaataca ccagaatgtt tgatttgcac tgtgcaaaaa
      601 tgcaatcaag ccaataagga ttctccaatt gaagggatgg ccagtgaccg accgaacgtg
      661 aggtattgcc gggcgaaata aatggcataa tgatttccgg aaataatcgg gcaagattaa
      721 aaccaagaat ggaagttgct ccaaagagga gaatggaggg tgtcacttct acggaatctt
      781 taaaacgata ttcgtgaatc aataatgcgg taatgcggtg aaattcctat tatccttgat
      841 tgctcatagc cataatcttc ctcccttacc agatctgatt cattttctta tgcccgacta
      901 aaaagcccag gccacaaaag agtatcgctc tcctttcgta atctctttga cttcatgtgg
      961 gtacataaag ttagaaggaa aaacgagcat atcccctgct ttgaatttga tctcatgctt
     1021 ggatgagaac aaaaattccc caccttcata atcctcattc aagattccta caagtgataa
     1081 tatgggtatg cccttatatt tcccgtcaaa aatcgaatga atatggtcat aatgttttct
     1141 catcatggta ccggtgcgat agcgattaaa ccgtattggc gtaaaagttt ggatgaaact
     1201 tttaattcgt tcatcttcct gatttgcaaa tttgatcatg tactcttcga cggattggca
     1261 aataaacggt gccaacgtat cttgcaaagc ttgcgtggta ttttgggtat ctaattcctt
     1321 ttccggctca gatttgaaat tatctgtgga ataactatac cagttatgtg gtttccattc
     1381 tttgccggta atttctttaa gaacatcctt gcaaataggc tgaggaatta catttgtcac
     1441 ataaatataa tcggacactt tatccatgac catctccaaa aaattcagat atccgacact
     1501 ccaggtgctg ccttaagatt ttatcctgcg cacattcgtg tgtaggtttt ttcctgttca
     1561 tgtggcttaa tgaaccataa gcgggatttt aatcctcccg gggactgtgt tgccagtcct
     1621 aatttcacat tggactggaa tcctttcttt gttaatgttc cgtcataaac cagccggcaa
     1681 tagacagtcg ttcgtggtct ccggcgagag catccacccg gcacactcga tgctgtcgag
     1741 gaacagcaaa aatgactagg gaaccggggg aaggaggaat gcaatatgtc acgtgtaatg
     1801 aactattcct ggaatctttt gagtctacag atgcaggaga gccatatatg ataagttccc
     1861 ctccccagtc cggatgccac tcctcatgga aataagtagc cactgccaga cgacggattg
     1921 gaggtcgccg gccctgggca taactataga tatcatcggt atcatcgtgg gttgaaagac
     1981 aatcacctgc accatacgaa taaaagttga aattgacgtg ccgcccttta atgttgggga
     2041 aagattcaat ataggcagca atgcaagaca taccgacacg ttggagaaag tgggcacctt
     2101 ccgaatgatc aatgtccgct ttacgccagt tcccgcaatt gggaaaggct tgagccactg
     2161 gcggcatatc ggacagagca gtccattttg atcgggccat tgcctcccga aaaacctttc
     2221 gttctccagg tttccaaaaa tcttctaaaa tcaacaccgg atcctcagca aacgaaaatc
     2281 gatcgggatt aaaacgagcg aaattatctt tcatgataat atgcacaaca acttataata
     2341 attcgaaatt ctggagtccc cttttagcaa gtttccctca tggtatcaaa ttcctcatag
     2401 gaagtgtgtg taaagaactc gtaccggatt tctccgttaa aagatgagat cccgcctcca
     2461 ttgaacatcc tcctattgct cctatgttgg tagggatatt ctccgctttc ggggagagga
     2521 ttgagcctat tcaggaaaat tctctttcga attttttctt cctgcaacca accaaaagat
     2581 accttcctta tccatttgga tatcggattt cctcactccg tcctccgata ataaataagg
     2641 ggataagtcg tcctccacaa ttttctctaa aattcaaggt gttattcaaa atatttgcag
     2701 aaaaacttta gcggaatttg tttggcaatc tttttgctat tccaaaaata gtatggaacc
     2761 acacttattt acagccggtg gcagaaaacc tcgtggcaaa cgtagtacac catatgggta
     2821 actcgatcct cttaattgac cacgatcgac tttttaggcg ggacgtacgg ctgtatttgg
     2881 agaaatgcgg attttactgt aaagaggcca atgacggacg ggaggccttg gccctgttgg
     2941 atggggggtt caacgtggat gtgatcctat caaagtacca gacgcctatt atcaatggac
     3001 tcagctttct caaagccttg tcctatcgcg tgaatggaca gcatgtccgc gtgattctcc
     3061 tgaatagtca tatgacaaac gaaatggaac atgaagcgaa gcaagcaggg gcctttgaag
     3121 tgctcgcaaa accgtatgat ctccgggaac ttctggagtt ggtagctcgt gcgtgcaagc
     3181 aatagattcc ccagttgttt ctgctagggc tgcatcggat ttcggagtgg aaagatctta
     3241 atgtatccca tatacatgtt tgtaaccatc ggggaggtgg atgcgtgagg ccaaaaattt
     3301 tactcatgga tgatgactct gaattacgtt caaccttacg ggatcgttta attatggaag
     3361 gctacgatgt tcaaaccgct cctgatggcc gattcgggtt gaagctctat catgaaacgc
     3421 cagttgatct ggtaattacc gatttactca tgcctgaaat ggatggtttg gaagtgatcc
     3481 gcgttctctg tgggacacct tcacctcctt tgatcattgc catgtcagga ggcgggatcc
     3541 acgatctgga tttcttggtg gaagcgacgg agtttggcgc gagtcgcact ctttccaaac
     3601 ccttccgcct ggacgacttc gttactcttg tcaatgaaat tctgaattca acgtcccatc
     3661 cgcatgtatg aatctccttg tcaattccga ggatcgactt tgggcataag tctgaagggt
     3721 tcaccacata cttactacgg catccctcat gggaagctga aaaatgatga acgatttggc
     3781 cattccaaat tcctggagga tggcagatct ccgtgaacca tacaagacc
//
LOCUS       DATIAL010000233         7173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1010410, whole genome shotgun sequence.
ACCESSION   DATIAL010000233 DATIAL010000000
VERSION     DATIAL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010410"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VLA60_07215"
     CDS             complement(<1..211)
                     /locus_tag="VLA60_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF09186.1"
                     /translation="MSLGRILALLSRHMYLYKRSFARLLEIFYWPFLDLVVWGFITIY
                     LEKVGMSLHGALSFFLGALILWDVLF"
     gene            complement(208..945)
                     /locus_tag="VLA60_07220"
     CDS             complement(208..945)
                     /locus_tag="VLA60_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09187.1"
                     /translation="MSSPVVEVRNLTKRFQGFTAVDNISFDIKKGEILGLLGPNGAGK
                     TTTIHMLLGLITPTVGNIHMFGLDLATHRETILKQVNFSSTYISMPFSLTVEENLKVI
                     ARLYELKHIQQRIDDIVKKLEMEDIRHRLTRKLSSGQMSRLTLAKAIMTEPKVLFLDE
                     PTASLDPDIVNKIKSFLKEYQRSEGLSILYTSHNMREMEEMSDRIIFLQRGKIVAEGT
                     ASEIIQRYAQRDLEEVFLKLAREQNGP"
     gene            complement(963..1661)
                     /locus_tag="VLA60_07225"
     CDS             complement(963..1661)
                     /locus_tag="VLA60_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HSF09188.1"
                     /translation="MGKGLTLIAATLLVLALGGAAAIPLTNQPTFCASCHTIRPSYDS
                     WIRSSHKDVTCVDCHVRPGISGFIQDKVFAGIEDVAITFFGTPTEPHNLESHVSSSVC
                     ISCHRAILRVTEVSVRDLPPPVKDVGLIMNHREHMEAFQKRGKGEGCTTCHGRVVHGA
                     PIKAYPIVIPRGHVKLDDQPHEPDHPKDSALWKANMADCLRCHDGEQRYEGEVLSNRC
                     ETCHLADKIGGFLF"
     gene            complement(1667..2170)
                     /gene="mobB"
                     /locus_tag="VLA60_07230"
     CDS             complement(1667..2170)
                     /gene="mobB"
                     /locus_tag="VLA60_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HSF09189.1"
                     /translation="MTPPILGFVGRSNSGKTTLIERLIPELTHAGYRVATIKHAGHGF
                     DLDTEGKDSWRHKRAGASTVIVLSKGSLAMFADVPEELPVDQVRDRFINGEIDLIIAE
                     GWKSQGFPKVIVVREQLQEVDVSLDGLLAVASMKRIECSAPWFDRDDVQGLAQLIMKK
                     FPLQRDS"
     gene            complement(2252..3481)
                     /gene="glp"
                     /locus_tag="VLA60_07235"
     CDS             complement(2252..3481)
                     /gene="glp"
                     /locus_tag="VLA60_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HSF09190.1"
                     /translation="MDQLTSLTSAQQTVLDAAHPLGCEKIGLLDSLGRVLGEDILAPR
                     SNPPWDNSAMDGFAVRWADIKQDYAITRIPELNIVEDVAAGAVATKTVGPGEAIRIMT
                     GAPMPAGADTVVRVEYTEPSGTKVRIMKTEAGQGSNIRPKGEDVTEGECIIPKGTQLR
                     SGEIGMLAILAKSFVLVSQRPRVAILSTGDELADLDESFDEHKIVNSNSYGIAAAVQE
                     SGGIPVLLGIAKDNPDSLKDKIRQGLTCDILVLSGGVSMGDYDFTKPVFAELGADMNF
                     WKLAIRPGQPVAFGKIQGKLAFGLPGNPVSSMVTFDQLVRPAMLKMSGHRKWERPVVK
                     ALFQETFSKHTDRRHFLRGILQQENGVLTVRTTGGQGSGILTSMVKANGFIDVPEEVE
                     SLKPGDWVNVQLLSRNF"
     gene            complement(3698..4663)
                     /locus_tag="VLA60_07240"
     CDS             complement(3698..4663)
                     /locus_tag="VLA60_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSF09191.1"
                     /translation="MATELNMIQPSSSSSGDVCTYMWACAICDETETCQKDREGHSRW
                     LVGKRMERIDYKILVMSNKGGVGKSTMTTNLAISLALKGYEVGICDMDIHGPNIPKMV
                     GAEGQKLKISTGGGIIPHQAYNLKIASMSFLLQNSDDPIIWRDAYKFEFINQLLGGVE
                     WQDLNFLLIDLPPGTGNESVTTIDLIGDVTGCVIVSTPQEVALLDSRKSVTFARDSEL
                     PIIGIVENMSGLDCPHCHQSIELFRKGGGEASAADMGVPFLGRVPLDPEMVLQCDRGE
                     PYALFHSDLPTADAIHGIANKVEEFCKKKDSLVKVSARPAPFKKA"
     gene            complement(4730..5209)
                     /locus_tag="VLA60_07245"
     CDS             complement(4730..5209)
                     /locus_tag="VLA60_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HSF09192.1"
                     /translation="MIRVAVVLISSKIQSGQKPDENRKPLERILTNHQLTLLSYDVVA
                     DDRQAIAHQLDTICETTAPHIILTLGGTGVRPTDWAPEATRDVIEKEIPGIGEAMRAE
                     SLKKVRTAMLSRGTAGIKGTTLIMNLPGSVTGVQENLNIVLPLLEHMVEKMGSMATS"
     gene            complement(5234..6190)
                     /gene="tatC"
                     /locus_tag="VLA60_07250"
     CDS             complement(5234..6190)
                     /gene="tatC"
                     /locus_tag="VLA60_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HSF09193.1"
                     /translation="MAKQSKIGSWFEEKVFKPLEDKKMPVMEHLQEFQWRLTRAVIVM
                     ACVFVGTFFYADTLVSWLRIPLQNYFILDSWEWMPSDLPKIPFVFLSPAEALWQNVKV
                     AGLCAVVLSTPHWLWEVWQFVLPGLHAQERRFVGPFTAISTVAFYLGLTFCFFVVLPF
                     ALHFLISYGLASGFIAQISIANYVGFILWFMLVFGLIFEVPLALTLMAKLGWVDAPIL
                     RKYRKWAFLGSFLFAAILTPTPDPFNQCIMAIPMYFFYEVGIISAQVFGKKKTPESED
                     AGASTPVSGPPAVRRPPLAPQPVGAGSGGDEEYVDVPDSGPR"
     gene            complement(6251..>7173)
                     /locus_tag="VLA60_07255"
     CDS             complement(6251..>7173)
                     /locus_tag="VLA60_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSF09194.1"
                     /translation="LRNTVSLGIISALGRGSVGITEYEDFIQTDAAINPGNSGGALVN
                     MKGELIGINTAIFSRTGGSEGVGFAIAVSIAKDIAASLIETGKVVRGWMGVAIQELTP
                     ALAQSFQLPEGRQGGVLISEVHEEGPSAKAGLQRGDVILEYGGEAVKDVNHLRNIVAR
                     TKVGKKKEIKVLREGKETLLTLELGERPSDQALAKTGPVEEEKAPAMAKLDNVLSGMT
                     VAPISGESRSEFNIPEQIKGVVVSKVESGSAVEAAGIQVGDVIQEVSRQSVKTIDDFK
                     QIASKIAKDELAVLLVNRRGNNLFIAVNPQ"
BASE COUNT         1798 a         1951 c         1645 g         1779 t
ORIGIN      
        1 gaaacaacac atcccagagg atcagcgccc caagaaaaaa tgagagggca ccatgcagcg
       61 acatcccaac tttttccaga taaatcgtaa tgaatcccca caccacgaga tctaggaacg
      121 gccaataaaa aatctccagc agccgggcaa agcttcgctt gtagagatac atatgacgcg
      181 ataataatgc aaggatacga ccgagactca tggaccgttt tgctctctag ccagtttcaa
      241 aaagacctct tccagatctc gctgagcgta gcgttgaatg atttcggagg cggtgccttc
      301 tgccacaatt ttgccccgtt gcaggaagat gatgcgatcg gacatctcct ccatctcccg
      361 catgttgtgt gaggtataga ggatactcag gccttccgaa cgttgatatt cttttaaaaa
      421 cgatttaatt ttattgacga tatcaggatc caaactggcg gtcggttcat cgagaaacag
      481 caccttgggt tcggtcatga tggccttggc cagggtgagc ctcgacattt gaccggaaga
      541 gagttttctc gtcagtcgat gccgaatgtc ctccatttcc aactttttaa caatatcatc
      601 aatgcgttgc tggatatgct tgagttcata gagacgcgca atgaccttga gattttcctc
      661 taccgttaag gaaaacggca tcgaaatgta ggtggaggaa aaattcacct gtttcagaat
      721 ggtttcccga tgtgtggcca gatccagtcc gaacatgtga atgttcccga ccgtcggcgt
      781 gatcagccct aacaacatat gaatggtggt ggttttgccg gcgccattcg gcccgaggag
      841 cccgaggatt tcaccttttt tgatgtcaaa agaaatatta tcgacggcgg taaagccctg
      901 aaaccgcttg gtgaggtttc gaacttcaac gacaggggaa gacatggaat ccgaggcagg
      961 catcaaaaca aaaacccacc gattttatcg gccaggtgac aggtttcaca gcgattgctc
     1021 agcacttcac cctcgtaacg ttgttcccca tcatggcaac gcagacaatc ggccatgttc
     1081 gccttccaga gcgccgagtc ttttggatga tctggctcat gtggttgatc atcgagtttg
     1141 acatgtccgc gcggaatcac aatgggatac gctttaatcg gcgctccgtg cacgacccgc
     1201 ccgtggcagg tcgtacaccc ctcccctttt ccgcgcttct gaaaggcctc catgtgttcc
     1261 cgatgattca tgatcagccc gacatctttg accggtgggg gcaaatcacg cacagagact
     1321 tccgtcaccc gtaagatggc acgatgacaa ctgatgcaca ctgaagaaga cacatgggat
     1381 tcaagattat gcggttcggt cggggttccg aaaaatgtaa tcgcgacgtc ttctattccg
     1441 gcaaagactt tatcctgaat aaacccggaa atgcctggac gcacgtggca atcgacacag
     1501 gtgacatctt tatgactgga tcgtatccag gaatcgtagg atggtctgat ggtatgacaa
     1561 ctcgcacaga aggtaggctg attggtcagc gggatagccg cggcccctcc taaggccagc
     1621 accaggagtg tggccgcaat gagggtcagg ccttttccca tgaacgttac gaatcccttt
     1681 gaaggggaaa ctttttcatg atcagttgcg ccagcccttg gacgtcatcc cgatcaaacc
     1741 agggagccga gcattcaatg cgtttcatag aagccaccgc caataaacca tccaacgaca
     1801 catccacttc ctgtagctgc tcgcgcacca cgatgacttt cggaaacccc tggcttttcc
     1861 agccttcggc aataattaaa tcaatttctc cattgatgaa tcggtcccgg acttgatcga
     1921 caggaagttc ttccggaaca tcagcaaaca tggccaggct accctttgat aagacaatga
     1981 ccgtactggc ccctgcgcgt ttgtggcgcc aactgtcttt gccttcagtg tcgagatcga
     2041 acccgtgccc ggcatgcttg atggtggcca ctcgataacc cgcgtgtgtc agttccggga
     2101 tgagccgttc aatcaaggtc gttttgccac tgttggagcg gccaacaaaa cccaaaatgg
     2161 gaggtgtcat gatgaagcct gtactccctt ctatgaggtc gaattatgcc aaatagacag
     2221 cgtgactgat ggaaaatata tcgtaggtct tttaaaaatt tcgtgataat aattggacgt
     2281 tcacccaatc gccaggcttc agagattcga cctcttccgg tacatcaatg aatccgtttg
     2341 ctttcaccat tgaggtgagg atgccggacc cctgcccccc ggttgtcctg accgtgagga
     2401 cgccgttttc ctgttgaaga atgccgcgga ggaaatgccg ccggtccgta tgtttggaga
     2461 acgtttcttg aaaaagcgcc ttcacaaccg gccgttccca ctttcgatgg ccgctcattt
     2521 tcaacatggc cggtcgcaca agctgatcaa aggtcaccat cgaagaaacg ggattccccg
     2581 gcaagccaaa cgccagcttc ccttggattt ttccaaaggc taccggctgc ccggggcgaa
     2641 tcgctaattt ccagaaattc atgtctgccc cgagttcggc aaacaccggc ttggtaaaat
     2701 cgtaatcccc catggagact ccgcccgata acaccaaaat atcacaggtg agcccctgcc
     2761 ggattttgtc ttttaaggaa tcagggttgt ctttggcaat tcccaagagg acagggatgc
     2821 cacctgactc ttgaaccgca gccgcaatac cataactgtt ggaattgacg attttatgct
     2881 catcaaatga ttcatccaga tccgccaact catcccctgt agaaaggatg gccactcgag
     2941 gacgctgcga cacgaggaca aaagatttgg ccagaatggc caacatgccg atttccccgg
     3001 aacgcagttg ggtccctttc ggaatgatgc actctccttc cgtaacatct tcccctttcg
     3061 gtctgatgtt tgaaccctga ccggcttcag tcttcatgat acgaaccttc gtccctgaag
     3121 gctccgtgta ttccacccgc acgacggtat ctgctccggc cggcattggg gccccggtca
     3181 tgatccgaat cgcttcccct ggaccgacgg ttttcgtggc cacggcccct gccgcaacat
     3241 cttccacgat gtttaattcg ggaatgcgag tgatggcata gtcctgctta atgtcggccc
     3301 accgtaccgc aaagccatcc atcgccgaat tgtcccacgg aggattggat cgtggggcga
     3361 gaatgtcttc ccccaaaacc ctcccgagag aatcaaggag cccgattttt tcacagccca
     3421 agggatgtgc cgcatccagg acagtttgtt gggcagaagt taacgaagta agctgatcca
     3481 tgtgtcttgc ctaatcatgc ggaaggttga agtggttccc tgaagacagc gacaccctac
     3541 tcagagtccc gccaggagca cacaaaatat atggttgttc atttcattac ggaagtcagt
     3601 acacattcaa tgcttgaact ctcgactatc ctctgccgtt ctaatcaaat agtcagatgg
     3661 aacggtgaac acatcgcgtt ccgctcaagg cgacctgtta ggcctttttg aatggagccg
     3721 gcctggccga caccttcacc agcgagtctt tctttttaca gaactcctcg actttgttcg
     3781 caatgccgtg gatggcgtca gccgtgggaa gatccgaatg aaacaacgca tagggctcgc
     3841 cacgatcaca ttggagaacc atttccggat ccagtggcac acgcccaagg aagggaacgc
     3901 ccatgtctgc ggccgaggct tcacccccac cttttctaaa caactcaatg gattggtggc
     3961 aatgggggca atctaacccg ctcatgtttt ccacaatccc aatgatggga agctcactat
     4021 ccctcgcgaa ggtgaccgac tttcgggaat ctaacagggc cacttcctgt ggagtggaca
     4081 caatgacgca acccgtgaca tcaccgatga gatcaatcgt cgtaaccgat tcattcccgg
     4141 tccctggcgg caggtcaatc aatagaaaat tgaggtcctg ccattctact ccacctaaca
     4201 attgattgat gaattcaaac ttatacgcat cccgccagat aatgggatca tccgaatttt
     4261 gtaaaagaaa tgacatggag gcgattttca gattataggc ttgatgcgga ataattccgc
     4321 cgcctgtact aattttgagt ttttggcctt cggctcccac cattttggga atattggggc
     4381 catgaatatc catgtcgcat attcccactt catagccttt gagggccaaa ctaatggcaa
     4441 gattggtggt catcgtgctt tttcccaccc cgcccttatt gctcatgacc aatattttgt
     4501 aatcaatgcg ctccatgcgc tttccgacca gccagcgact atgtccttcg cggtcctttt
     4561 ggcaggtttc ggtttcatca caaatggcac acgcccacat ataggtacag acatccccac
     4621 tcgaactcga ggatggttga atcatattca gttcagttgc catgaatccc tccacatacg
     4681 tccactacga tgatagtttg gataatataa attcggccac acgcatatgt catgacgtgg
     4741 ccatacttcc cattttctcc accatatgct ctaacagcgg tagaacgata tttaaatttt
     4801 cctgcacgcc ggttacgctt cctggcaaat tcataatcag tgtggtcccc ttaattccag
     4861 ccgttccgcg tgagagcatg gcagtcctga cttttttaag actttcagcc ctcatggctt
     4921 ctccgatgcc gggaatctct ttttctatca cgtccctggt tgcctctggc gcccaatcgg
     4981 ttggtcgcac acccgttcct ccaagagtca aaatgatatg aggcgcggtc gtctcacaaa
     5041 ttgtgtccaa ctggtgggca attgcctgac gatcatccgc cacgacatca taggataaaa
     5101 gggtcaattg gtggttcgtc aagatccttt ccaaaggttt ccgattctca tcaggttttt
     5161 ggcctgactg tattttgctg ctgatcaaca ccacagccac ccgtatcatc aaatgcctct
     5221 gctggatttc ctattaccgc ggacctgagt ccggaacgtc gacatattcc tcatccccac
     5281 cagacccggc tcccactggt tggggggcta gtggaggacg ccgaactgcc ggcggcccag
     5341 agaccggggt ggatgcccct gcatcctcac tttctggagt ttttttcttg ccaaaaactt
     5401 gggcgctgat aatacccact tcataaaaga aatacatcgg gatggccata atacattggt
     5461 tgaatgggtc aggcgtgggc gtaagaatgg ccgcgaatag aaaagatccc agaaatgccc
     5521 atttccggta ttttcgtaat ataggagcat ccacccaccc taatttcgcc atcaaggtca
     5581 aggcgagcgg aacttcaaaa atcagtccaa agaccaacat gaaccacagt ataaaaccca
     5641 cataattggc tatagaaatt tgtgcaataa atcccgaagc caacccatag gaaatcaaaa
     5701 aatgcaacgc gaagggcaac acgacaaaaa aacagaaggt caatcccaaa taaaaggcca
     5761 cggtgctaat ggcggtgaaa ggtccgacaa accggcgttc ctgggcatgt aatcccggaa
     5821 gaacaaattg ccagacttcc cacaaccaat gaggggtcga caaaacaact gcacataaac
     5881 cagctacttt cacattttgc cataaggctt cagccgggga gaggaagacg aagggaattt
     5941 ttggcaaatc cgatggcatc cattcccatg aatctaaaat gaaataattt tgcagaggga
     6001 tccgtagcca ggacactagc gtatccgcat aaaaaaatgt cccgacaaaa acacaggcca
     6061 taacaatgac cgcacgtgtg agtcgccatt ggaactcttg caaatgctcc atgacgggca
     6121 tttttttgtc ttccagtggc ttaaagactt tctcttcaaa ccaggaacca attttgcttt
     6181 gtttagccat gtgagtcctg cgccacaaaa atccgagggg ggctcgttcg cccccctcat
     6241 tacttatccc ttattgagga ttcactgcga taaacagatt attgccgcgc cggttgacca
     6301 acaaaaccgc taattcatct ttggcaattt ttgacgcaat ctgcttgaag tcgtcaatcg
     6361 ttttcacgct ttggcgactc acttcttgaa tgacgtcacc aacttgaata ccagcagcct
     6421 caacggcgct gcctgattcg actttcgaga cgaccacacc tttgatttgt tcaggaatat
     6481 tgaattcact ccggctttcc ccactaatcg gggcaacggt cattcccgac aatacgttat
     6541 ccagtttggc catagccgga gctttctctt cttctaccgg cccagtcttg gccagtgctt
     6601 gatcggatgg ccgttctccc agttccaagg tcagcagggt ttctttgccc tcgcgtaaca
     6661 ctttaatttc ttttttcttt ccgacctttg ttcgggcaac aatgtttcga agatgattga
     6721 catccttgac ggcttctccg ccgtattcaa ggattacatc gccccgttga agtccggcct
     6781 tggcggaagg gccttcttca tgtacttcac tgattaatac cccaccctgt cgaccttcag
     6841 gtaattgaaa cgattgcgct aaggcaggcg ttaattcctg aatggccact cccatccatc
     6901 ctctaactac cttcccggtt tcgatgaggc ttgcggcaat atccttcgca atactgactg
     6961 cgatcgcaaa tcccacgcct tcggaaccac ccgtcctgga aaaaatggcc gtgttgatcc
     7021 cgataagctc ccctttcata ttcaccaggg ccccacctga atttcccgga ttaatcgcgg
     7081 catccgtttg aatgaagtct tcatattccg taatacctac actacctcgg ccgagggcac
     7141 tgataattcc cagagacacg gtattgcgta atc
//
LOCUS       DATIAL010000234         6742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_43303, whole genome shotgun sequence.
ACCESSION   DATIAL010000234 DATIAL010000000
VERSION     DATIAL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43303"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VLA60_07260"
     CDS             <1..1117
                     /locus_tag="VLA60_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09195.1"
                     /translation="NEPKNVCFDAEGNLLIADSENHVIRRLRHSTGVIETVAGCMVIR
                     RVGEENPQPPTPSLTSHVEEEIDPLADVDEIPGQAYSQTPDLSGTVRYIVGQGMGKDR
                     FAGDGALATRAQLNFPSAVAVDAAGNLFIADTWNHRIRRVDSKTGMIQTVAGTGQAKW
                     TGDGGPGASASLNEPVALVVDGTRLYIADQSNNRIRMLDLESGVIITIAGTGESGYTG
                     DGMPAIESALAGPSGLALDRHGNLYIADTFNGRIRMLDHRTGMLSTVAGDGAEFRYER
                     GANEQSQALARPYGIAITPQGHVLITDSDHHLIRKWDAVNKEMTLVAGNGQSTWAGDG
                     GPSSDSSLSFPFGVAVDVLGNIAIADTFNHRIRYIPV"
     gene            1291..2304
                     /locus_tag="VLA60_07265"
     CDS             1291..2304
                     /locus_tag="VLA60_07265"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HSF09196.1"
                     /translation="MIKKKSAYTVAVVGATGAVGTEMIQVLEERDFPVEELVPLASAR
                     SEGNEVMFRGQTLPVKVLEHNSFEGVDVALFSAGAGVSTEFGPMAVKAGAVVVDNSSA
                     WRMDPNVPLVVPEVNVHALSGHQGLIANPNCSTIQMVVALKPLHDAAQIKRIIVTTFQ
                     SVSGTGKDAMEELIDQSRQLLSFGEAKVSVYPHQIAFNCLPHIDDFLPNGYTKEEMKM
                     VNETRKILGESSLPITATTVRVPVFICHAESVNVETEQKLSANAARAILSAAPGIQVY
                     DDPSRNLYPLPIDVVGTDAVFVGRVREDESVTNGLNLWIVADNLRKGAALNAVQIAEE
                     LIR"
     gene            2308..2559
                     /locus_tag="VLA60_07270"
     CDS             2308..2559
                     /locus_tag="VLA60_07270"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HSF09197.1"
                     /translation="MRVKMMSGVGKALMLLGTILLVLGGIFWALGKWSDGEGGLGWLG
                     RLPGDFIIKRDHVTFYFPLATSLILSVVGSVIFYLFFRR"
     gene            2569..3765
                     /locus_tag="VLA60_07275"
     CDS             2569..3765
                     /locus_tag="VLA60_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HSF09198.1"
                     /translation="MACVTHRLCLGLLLVSWVMLYSPVIWADEPAIRVGLAQGAQVLS
                     IQSANSFSLLLPSGKRFHVSGRVTIRRQGAGLLLNERVVTESSLRVEGSGGIYQVGVE
                     TKNEKAALRNSRPEWVVRGPLEIRLDPSGLTLINWVDLESYVAGVVSGEVGAKWPLEA
                     LKAQAVAARTYVLYKKVENQLQSFDVVAGVQDQVYHGHSVQAESVLQAVAATRGQVVT
                     YENRPIFAAYSSTAAGPTEDALYVWALDLPYLKGVDCPFDEQAPRYDWRTSFTFDFLE
                     QQLLKEGYDVGKIATFTPYTFTPSGRVDRVRLLHSRGEIILRGQDLRRVVGYSKIFST
                     QFSIESLGQEVVVVGHGAGHAVGMCQWGMREMAELGYDYQSIIRHYYPHTILLPIDRV
                     LLTATD"
     gene            3794..4813
                     /gene="queA"
                     /locus_tag="VLA60_07280"
     CDS             3794..4813
                     /gene="queA"
                     /locus_tag="VLA60_07280"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HSF09199.1"
                     /translation="MDLSQFNFPFDQTLVAKYPVHPRDHARLLVLTRSTGDITDRQVK
                     DLPNILRPGDLLVVNNTKVIPARLWAKKVPSGGRVELLFVKEIGEDHAEVLIQGKVGV
                     GQIVECEGSARAQVVEKEPGRTVIHWLGPGALRTWLLSHGEIPLPPYLKRQPVSRDQE
                     DYQTVFAKVEGAIAAPTAGLHFTPQLITQLAEKGIGTATVTLHVGPGTFQPVKTTDIT
                     RHIMHPEWFEVSEETAERIREVRKRGGRIVAVGTTVTRSLESALDETGELRPCSGETR
                     LFILPGFKFRVIDGLLTNFHFPETTLLMLVAAFAGLEEARKAYVHAVCERYRFYSYGD
                     AMLIL"
     gene            complement(4997..5698)
                     /locus_tag="VLA60_07285"
     CDS             complement(4997..5698)
                     /locus_tag="VLA60_07285"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HSF09200.1"
                     /translation="MIRIIREGSAKYPWILKLLILGIAVTFVIGMGWYGYESTQGNAV
                     ATVGPYKVTKDEYLRTKQGYFRFYRDQLKQEDVKEETIKQLALEGLITTKSWQLLADE
                     MNLAVSAQELHDAIVNQKDFQKDGVFDTEYYQRLLAANRMKPHQYEEQRRNELLAEKA
                     RLLVSEATTLTPTEMKEVKELADRQTLDGAEPDLTVLEQIRLQFLMQKKQRAMQAFQT
                     ALRARGDVTIHEELL"
     gene            6033..>6742
                     /gene="gltX"
                     /locus_tag="VLA60_07290"
     CDS             6033..>6742
                     /gene="gltX"
                     /locus_tag="VLA60_07290"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HSF09201.1"
                     /translation="MSEVRVRFAPSPTGFLHIGGVRTALFNWLFARHGGGTFILRIED
                     TDRDRSTDDAIQVILDGLKWTGLNWDEGPFRQTDRLELYRDRAMDLLNRKLAYWCVCT
                     PEELETRRKDAQAKGESIKYDGRCRHRGISDPAEPAALRFLTPQEGQTVVNDLIKGQI
                     VFDNTVMDDLIILRSNGYPTYNFSVVVDDGLMNISHVIRGDDHVNNTPRQVPLFSALG
                     FPVPQFAHLPMILGSDKTR"
BASE COUNT         1501 a         1563 c         1939 g         1739 t
ORIGIN      
        1 gaatgaaccc aagaatgtgt gctttgacgc tgaaggcaac ctcctgattg ccgattccga
       61 aaatcacgtc attcgccgtt tgcgacattc gaccggtgtc atcgaaacgg tagccgggtg
      121 tatggtcatc cgccgcgtcg gggaagaaaa tccacagccc ccgaccccat ctctaacttc
      181 tcacgtcgag gaggagattg atcctcttgc ggatgtggat gagattccgg gccaggccta
      241 ctctcaaacg cctgatttga gtggaacggt gcggtacata gttggacagg ggatgggcaa
      301 agatcggttt gcgggagatg gagccctggc cacaagagca caattaaact ttccctctgc
      361 cgtcgccgtt gatgcggcag gcaatctctt tattgccgat acgtggaacc atcgcattcg
      421 gcgtgtagat tctaaaacgg ggatgatcca gaccgtggcc gggaccggcc aggcaaagtg
      481 gacgggggac ggcggtcccg gagcttccgc ttccttgaat gaaccggtgg cattagtcgt
      541 ggatggtacg cgactctata ttgccgatca gagtaataat cgcatccgta tgctggattt
      601 ggaatcgggt gtcatcataa ccatcgcagg aaccggagag tccggatata ccggggatgg
      661 catgcctgcg attgagtctg cgcttgcggg accgagcggg ttggcacttg atcgccatgg
      721 aaatctctat attgctgata cctttaatgg ccgtattcga atgcttgacc accgcacggg
      781 gatgctgtcc acggtggcgg gggatggcgc cgagtttcgc tacgagcgtg gggcgaatga
      841 acaatctcag gcgttggcca ggccttatgg catcgcgata acaccacaag gacatgtgct
      901 tatcacggat tccgatcatc acttaatccg gaagtgggat gccgtcaata aagaaatgac
      961 cctggtcgcc ggcaatggcc aatcgacatg ggctggtgat ggaggtccgt cttcggatag
     1021 tagcctcagt tttccgtttg gtgtggctgt tgacgtcttg ggcaatattg cgattgccga
     1081 tacctttaat caccgcatcc gttatattcc tgtctaagaa gagccccgga ggccatcacg
     1141 gggctttcag aattttcggc ctcgggcgtt ggctggaacg gacttctgtt acgggtttcc
     1201 ctttcgttat ttttgagtca aggattttga gctgatagga tgtatcttat actgaatgct
     1261 atccgttttt cttgagggag tgagcctgac atgataaaga agaaatcggc ctatacggtc
     1321 gcagtggtgg gagcaaccgg tgccgttggt acggaaatga tccaggtcct ggaagaacgg
     1381 gattttcctg ttgaggagtt agtccccttg gcctcggccc gttccgaagg caatgaggtg
     1441 atgtttcgcg gtcagaccct tcccgtcaag gtgctggaac acaactcgtt cgagggtgtg
     1501 gatgtggcct tgttttcggc aggagccggt gtcagcacag aattcggtcc gatggctgtc
     1561 aaagctggag ccgtggtggt ggacaatagt tccgcatgga ggatggaccc caacgtacca
     1621 ctcgtggtgc ctgaggtgaa tgtccatgct ctttcgggac atcaggggct gattgccaat
     1681 cccaattgtt ccaccattca aatggtcgtg gctttgaagc cgctgcatga tgcagcccaa
     1741 atcaaacgga tcattgtcac gacgtttcaa tccgtgtccg gaaccggcaa ggatgcgatg
     1801 gaagaattga ttgatcagtc ccggcaacta ctcagttttg gagaagccaa agtgtccgtc
     1861 tatccgcatc aaattgcctt taattgtttg ccgcatattg atgatttttt accgaatgga
     1921 tacaccaaag aagaaatgaa aatggtcaac gaaacccgga aaattctggg tgaatcgtcg
     1981 ttgccgatta cggcaacgac agtcagggtc ccggtcttta tctgccatgc ggaatccgtg
     2041 aatgttgaaa ctgagcaaaa gctgagtgcc aatgccgcac gcgctatact gtccgccgct
     2101 ccgggcattc aagtctacga tgatcccagt cgcaatttgt atcctctgcc gatcgatgtg
     2161 gtgggaactg atgcggtatt tgtgggtcgg gtgagggaag atgaatcggt gactaatgga
     2221 ctgaatctgt ggattgtggc ggataatttg cgaaaaggtg ccgcgctcaa cgctgtacaa
     2281 atcgccgagg aactcatccg gtaatgtgtg agagtcaaga tgatgagtgg agtgggaaaa
     2341 gccctgatgc tgctggggac tatcctcctg gtgctggggg gaatattttg ggctttgggg
     2401 aaatggtccg acggtgaggg cggcttgggc tggcttggcc ggttgcccgg ggattttatc
     2461 atcaaacggg accatgtgac gttttacttt cctcttgcga ccagtctcat tctcagtgtg
     2521 gtcggaagtg tgatcttcta tcttttcttc cggcggtagg caagagttat ggcatgcgtg
     2581 acacatcgct tgtgcctagg tctactcctg gtgagttggg tgatgttgta ttctccggtc
     2641 atttgggcgg atgaaccggc tattcgcgtg ggtcttgccc agggtgccca ggttctttcc
     2701 atccaatctg cgaattcgtt ttccctcctt ctgccttccg ggaaaagatt tcatgtttca
     2761 ggtcgagtga ctattcgaag gcaaggcgct ggtcttcttc tcaatgaacg tgtcgtgact
     2821 gagtcgagtc tgcgggttga gggatccgga ggtatctatc aggtaggagt ggagacgaaa
     2881 aacgaaaagg ctgcgctccg gaattcacgg ccagaatggg tggttcgcgg cccgcttgaa
     2941 attcgattgg atccatccgg attaaccttg attaattggg ttgatcttga atcatatgtt
     3001 gcaggcgtgg tttcgggtga agtcggggcc aagtggccct tggaggccct gaaagctcaa
     3061 gctgttgctg ccagaaccta tgtgttgtac aaaaaagttg aaaaccaatt gcaatccttc
     3121 gatgtggtcg ccggcgtgca agatcaagtc tatcatggcc attccgtaca agccgaatcc
     3181 gtgcttcagg ccgttgcagc caccaggggc caagtggtga cgtatgaaaa tcgccccatc
     3241 tttgcggctt attcgtcgac ggccgctggt cccaccgagg atgcgttgta tgtgtgggca
     3301 ttggatcttc cctatctcaa aggtgtcgac tgcccctttg acgagcaagc tccacgatat
     3361 gattggcgta catcatttac gttcgacttt cttgagcagc aactgctcaa agaggggtat
     3421 gacgttggca aaatcgcgac ctttacgccc tataccttta cgccatcggg tcgggtggat
     3481 cgtgtgcggc tgctccattc tcgtggagaa attattcttc gcgggcaaga cttgcgacga
     3541 gtggtcggat actccaaaat tttcagcacc caattttcca tcgaatcatt ggggcaggaa
     3601 gtggtcgtgg tgggtcatgg ggcgggacat gccgtgggga tgtgccagtg gggtatgcgc
     3661 gagatggccg agttagggta tgactaccaa tcgattatcc gacattatta cccccacacg
     3721 atacttcttc ctattgaccg tgtgctcctc actgcgaccg actaattctg ttaccttttt
     3781 ttcttgtctg atcatggatc tttcccaatt caactttccc tttgaccaga ccctggttgc
     3841 caaatatccg gttcatccac gcgatcatgc caggttattg gtgctgaccc gatctactgg
     3901 agatatcacc gatcggcaag tcaaagatct cccaaatata ttacgacctg gggatttgct
     3961 cgtggtgaac aataccaaag tcattccggc tcgcctctgg gcgaagaagg tcccaagtgg
     4021 gggccgtgta gaattgctgt ttgtcaaaga aatcggtgaa gatcatgcgg aagtcctcat
     4081 tcagggaaaa gttggagtcg gacagattgt ggaatgtgag ggatctgccc gtgcgcaggt
     4141 cgtggaaaaa gagcctggtc gaacggtgat tcattggctt ggtccaggtg cattgcggac
     4201 atggttactg tcccatggag aaatccccct tcctccgtac ttgaaacgcc aaccggtttc
     4261 acgcgatcaa gaggattatc aaaccgtctt tgctaaagta gaaggggcta ttgccgcgcc
     4321 aaccgccgga ttgcatttta cgccacaatt gatcacgcaa ctggcagaga aggggatcgg
     4381 gacggccacg gttaccctgc atgttggacc tggaactttt cagccggtca agacaactga
     4441 cattacacgg cacatcatgc atccggaatg gttcgaggtg tcagaggaga ccgctgagag
     4501 gattcgtgaa gttcggaagc gaggcgggag aattgtcgct gtcgggacga ccgtgacacg
     4561 aagtcttgag tcagctctag atgagaccgg cgagctcagg ccctgttcag gagagacccg
     4621 cctcttcatc ctgccgggat tcaagttccg ggtcattgat gggctcctga ctaattttca
     4681 ttttcccgaa acgactcttt tgatgttagt tgcggcattt gcgggattag aggaggccag
     4741 gaaggcgtat gtccatgccg tgtgtgagcg ctatcggttc tatagctatg gggatgccat
     4801 gcttattctt taataatggc gcatggcaga aatagagaaa aatccggaag ttatcctcgg
     4861 taaagactat cttacgtagg gcaaaggtct caggttatgg aagcatgaag gagggttctg
     4921 tggatagcat aaaaggatgg ctcagtgggt gactccaaac agatgagatg gtcagggtaa
     4981 ggccggagac tctttcttac agcagttcct cgtgaatcgt aacgtctcca cgggctcgaa
     5041 gagccgtctg gaaggcctgc atggctcgct gtttcttttg catgaggaat tgaaggcgaa
     5101 tctgttccag gacggtgagg tcgggctcgg ccccatcaag cgtttgtcgg tcagcgagtt
     5161 ctttgacttc tttcatttcc gtcggggtga gtgttgtggc ttcagagaca aggagtctcg
     5221 ccttttccgc cagcaattcg ttccggcgtt gctcctcata ttgatgaggt ttcatccggt
     5281 tcgccgccag aagtcgctgg taatattccg tatcgaagac gccatctttt tggaagtctt
     5341 tttggttcac aatcgcatcg tggagttctt gggccgagac cgctaaattc atttcatccg
     5401 ccagtaattg ccaggatttt gtagtgatca aaccctcgag agcgagttgc ttaatggtct
     5461 cttcttttac gtcttcttgt ttgagttggt cgcgatagaa tcggaaatag ccttgcttcg
     5521 tccggagata ttcatccttg gtgactttat aggggccgac ggttgccacc gcgttgcctt
     5581 gagtggattc gtacccatac caacccatgc cgatgacaaa ggtgacagcg atgcccagga
     5641 tgagtaactt gagaatccag ggatatttag cggaaccctc gcgtataatt cgaatcatgc
     5701 gtcatgcctc ctcagaattt ctttgatgac catgatgaaa ttttaagggc cggtaacggg
     5761 aaaggcaagt gagcaggccc tgccaattca gttctttaca tcggtaaggc ggcatggtaa
     5821 agcaacaggc cggagaatga aaaatatgac ttgggaggtc atcctgcatg cggtatgaaa
     5881 atttcccgtt aaaggctgtc tttggttctc atgggaaaga ggtgtggaga ggcgatgatg
     5941 gcgccatgtg aaatcaatgg cacctatccg gagcggcctc ttttaatatg ggcgtgggtg
     6001 gtcaagagca agagaaagag cggtgaaaac acatgagtga ggttcgagtt cgctttgcgc
     6061 caagtccgac tggatttctc catattggtg gggtgcggac agcgttgttt aattggttat
     6121 ttgcgcgtca tgggggtgga acctttatcc ttcgtattga ggacaccgat cgggaccggt
     6181 ccacggatga tgctattcag gttattctgg atggcttgaa atggaccggg ttgaattggg
     6241 atgaagggcc tttccgccaa accgaccgct tagagctgta tcgtgaccgg gcgatggatc
     6301 tcttgaatcg aaagctggcc tactggtgcg tctgtacgcc tgaggaatta gagacgagac
     6361 gaaaagatgc tcaggctaag ggcgaatcca tcaaatatga tgggcggtgt cgacatcgag
     6421 gtatttccga ccctgccgaa ccggcggcgc ttcgctttct aaccccgcag gaagggcaga
     6481 cagtcgtcaa tgatctcatt aaaggccaga ttgtatttga taacacggtc atggacgatc
     6541 tcatcatcct caggtccaat ggctatccca cctataattt ttcggtggtg gtggatgatg
     6601 gacttatgaa catttcccat gtcattcgcg gggatgatca tgtcaacaac accccgcgac
     6661 aggtgccgct cttttcagct ctggggtttc cggtgcctca gtttgctcac ctgcccatga
     6721 ttctggggtc tgataaaacg cg
//
LOCUS       DATIAL010000235         2833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1549020, whole genome shotgun sequence.
ACCESSION   DATIAL010000235 DATIAL010000000
VERSION     DATIAL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549020"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1008)
                     /locus_tag="VLA60_07295"
     CDS             complement(232..1008)
                     /locus_tag="VLA60_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09202.1"
                     /translation="MNLVDRRRKRTSVWLSAAFTGVCILGTGQVEAAKMPKVEICHFD
                     ADEGVFKKIAVSGNAVQSHLANHGDIFPASSNGVGTITLDAECNEVIARHVFSRAYID
                     RDRDGTYDPQVDVEISELVDTNMDGVINAGDTVEFGQYPKTFDPCSSPPDAICPDLGN
                     LNPAPVVVTHVFSATPTSVIVQSASGSLFNWEIPGGLEIVRLGTIPEQILFDSFLDQP
                     NDAIKIPLGGEADPSDAVDIMRLGNGVNDYFFNVEINLAP"
     gene            1288..1491
                     /locus_tag="VLA60_07300"
     CDS             1288..1491
                     /locus_tag="VLA60_07300"
                     /inference="COORDINATES: protein motif:HMM:NF025846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09203.1"
                     /translation="MNGRLKQAIQAGEPVAFTQLWTPDQELDMTAAFGKTELGNLTGA
                     KELLGNLYGYDQLRLSRAVHGKT"
     gene            complement(1825..2253)
                     /locus_tag="VLA60_07305"
     CDS             complement(1825..2253)
                     /locus_tag="VLA60_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09204.1"
                     /translation="MNQDERCAQLVALDDELLKGGVILSEWCCFIVREADTAFASGAN
                     LASILTAVSGIETYLRSEHSETGKERLVKLINRASIDDNLKNDLHTLRKYRNKWVHID
                     DPWDDAGLLERPEDTEFELEQMALFAAHALRRTIYENQWV"
     gene            complement(2468..>2833)
                     /locus_tag="VLA60_07310"
     CDS             complement(2468..>2833)
                     /locus_tag="VLA60_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSF09205.1"
                     /translation="NIIPGLEKALAGKGEGDALKVRIEPADAYGEVIPDGIKSIERAA
                     FEGVDSVEPGMVFEAQAPDGTTQQIMVVKVDGDKVTIDTNHPLAGIALNFDIKVLSVR
                     EATKQELEHGHTHEHGHDH"
BASE COUNT          710 a          741 c          679 g          703 t
ORIGIN      
        1 tcagatctca tttcaggcat tagttattcc aggtgggtgc ctcacgagcg tattggagag
       61 tagtggggaa ggtgtttacg ttggctgcaa ttcgaaattt catatgaggg gccacggcag
      121 gcgcggccgg tttatgttgc ccacgagttt gcttccggta catgaaaaag gtcagacccg
      181 gacgagccct tttatccgca ggataaaagg gctcgctaat gggcaatcac actaaggagc
      241 gaggttgatt tcgacgttaa agaagtagtc attgacgccg tttcctagcc tcataatatc
      301 tacggcatca ctcggatcgg cttccccccc taagggaatt ttaatagcat cattcggttg
      361 atcaagaaaa ctatcaaata gtatttgctc gggtatggtt cctaaccgca ctatttccag
      421 tccacctgga atttcccagt tgaagagcga acctgaagcc gattgtacga tgacggaagt
      481 gggcgtggcg gaaaaaacgt gggtaacaac caccggagcg ggattgagat tgccaagatc
      541 tgggcaaatg gcgtcggggg gagatgagca agggtcaaac gtctttgggt attggccgaa
      601 ttcaacagtg tcgcccgcat taataacgcc gtccatgttg gtatcaacaa gctccgagat
      661 ttcaacgtcg acttgcgggt cgtacgttcc gtcccgatct ctatcgatgt atgcccggga
      721 aaaaacatgc ctcgcgatga cctcgttgca ttctgcatcc agggtgatcg ttccaacacc
      781 atttgaactt gcggggaata tgtccccgtg gttggccagg tgactctgga ctgcgttacc
      841 actgacggca attttcttaa aaaccccttc atccgcgtca aaatggcaaa tctccacttt
      901 aggcatcttc gcagcttcaa cttgacccgt tcctagaata cacacccccg taaaagctgc
      961 actgagccat accgatgttc ttttccttct tcgatcgacc aaattcataa catcctccaa
     1021 atgtgtggtt atgaataata catcggttgg ttattcaaaa aaggaaagta ccacggagca
     1081 aaataatttc acccacggtg gaaaatcgta aatacaaaca gatccaccct cccattagta
     1141 agtcgcgcac gttctggctt gggatgcttc aaccaatggt caagggcaaa gcccctgagt
     1201 ggcaccatca caaaaaaaca ctacgcttat ttaaagggga tttctgtagg cggcattgcc
     1261 aatcaacggg gatgagcggc cagtactatc aatgggcggc tgaaacaagc tatccaagcg
     1321 ggggagccgg tagcctttac ccaactgtgg acaccagatc aggaattaga catgacagcg
     1381 gcctttggga aaacagagct tggaaatctc accggcgcca aagaactact cgggaatctc
     1441 tacggttacg accaactccg cctctcccgc gccgttcatg ggaaaacctg agatattgta
     1501 atgcaaacaa gaaatgactt ttaacacaaa tagttttcaa ttcaagcgtc atcctgagcc
     1561 aagggcgaag gatctgtatt caaattgaag acgctctggc atgcgatatc cttcctggaa
     1621 attaccctga ctttgctgaa gggcccagga taacttagtc tctctccttt tgcattcgcc
     1681 tgaacagaaa ataaactcgt ctggctcatt gtctatgagg acattgaatc ctgctgtgcc
     1741 cttcttgggg aacctgaggc ccaaccgccg agttcaccca cccagtttta cgtcgtcggc
     1801 tgcagcgatg ggttaccaga agcgctacac ccactgattt tcataaatag ttctgcgcag
     1861 cgcgtgggcc gcgaagagtg ccatctgctc aagttcgaac tcagtatctt ccggtcgttc
     1921 gagaaggccc gcatcgtccc acggatcatc gatatgcacc cacttattgc ggtatttgcg
     1981 caaagtgtgg agatcatttt tcaggttgtc atcgattgaa gctcgattga ttaacttaac
     2041 gaggcgttct tttccagttt ccgaatgctc agaccggagg taggtctcga ttccagaaac
     2101 agcagtgagg atcgacgcaa ggtttgcccc actagcgaat gcagtatcgg cttctcgaac
     2161 gatgaagcaa caccattccg aaaggatgac accgcctttg agcagttcat catctagcgc
     2221 cacaagctgc gcacagcgtt cgtcttggtt catgtcggct aacgacttgt cgagcagcgg
     2281 tgcggcaaag ccgcgtccgt ctgaattgct ttgttagagt tttccatcat tcatatataa
     2341 tcctgcggga tgttcaaaaa ggctcgtcca gcaaggccgc aggtgattgg gcgagcggag
     2401 catacgctaa gtacgtgagc acgacaaagc gccgagaacg ccgctggcga cctttttcaa
     2461 catcctgcta gtggtcatgc ccgtgctcat gagtatgccc atgctcaagt tcttgctttg
     2521 tcgcttctct cacactcaga actttaatat caaagttgag cgcgataccg gccagtggat
     2581 gattggtatc aatggtgact ttatccccat ctacttttac caccataatt tgctgcgtcg
     2641 tgccgtccgg cgcctgggcc tcaaacacca ttcccggttc aaccgaatct accccctcaa
     2701 acgcagccct ttcgatgctc ttgatcccgt caggaatgac ctctccatag gcatcggcag
     2761 gctcaatcct taccttcaga gcatcaccct ctcctttacc tgccagcgct ttctccagcc
     2821 cggggatgat att
//
LOCUS       DATIAL010000236        11632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_903302, whole genome shotgun sequence.
ACCESSION   DATIAL010000236 DATIAL010000000
VERSION     DATIAL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903302"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..767
                     /locus_tag="VLA60_07315"
     CDS             111..767
                     /locus_tag="VLA60_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HSF09206.1"
                     /translation="MMVKSLIYVVDPMCSWCWGFSPVIDEIVRRYRDRVTIEVLLGGL
                     RPGNTERFDERRRAYILSHWHAVHERTGQPFNFNFHPEPSFMYDTEPPSRAMMVIRQL
                     APDKEFVFLHAVQEAFYVKNQDVTHPEILADLAGTQGIDQDRFLEWFHDSAMKQSVWQ
                     EFDRARQLGVSGFPALLGQNGNGHIRLTQGYQPTGVMVSLIDKWLDGPASGNGSGSIG
                     "
     gene            880..1518
                     /locus_tag="VLA60_07320"
     CDS             880..1518
                     /locus_tag="VLA60_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HSF09207.1"
                     /translation="MRKVSKVCFGIGTALIVGFGFVTASVAEMAKWKLDHDHSRVGFQ
                     VAHMVVSKTNGKFTEYSGMVEMDAEEKEFKTIEAVIQTASVTTDHQKRDEHLRSPDFF
                     DVQKFPTMTYTMKSYKKTGDEYTATGDLTLLGVTREITLVGTFNGVAQDPWGNTRAGF
                     TASGTLNRKDFGMKFSKLLDSGGMLVGDEVNIILEIEVIKEKTVEKVKQEKE"
     gene            complement(1696..3237)
                     /locus_tag="VLA60_07325"
     CDS             complement(1696..3237)
                     /locus_tag="VLA60_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HSF09208.1"
                     /translation="MLRFPIVQFMIGLALTTPVLGFDPQPSSSPSMPMVIGNIIQDIR
                     ELSHPSYQGRQAGTDGSLQSADYLVDRFRSLGLQPANHVSKHEQDFHWLQQRFMTAVQ
                     LLEPSTLTLSLIGANRTSMTMSLVPGQEFLPILDSPSTRTTARVVFVGYGIVDPARGI
                     DEYQGLNVDNRIVLFLRGKPPSYPGWITHEEKAAIAKERGAVGYLTVTGPLLGRYEAR
                     KGLGQGPLAIYGETPDNRPIPGAWIHGKLLDQALNAANDSLEALQQAANDIGTFRSSP
                     LPLLVQFHWESHSQPGTLTNVIGMLPGRDQELRKELILIGAHRDHFGQQAGLLFPGAD
                     DNASGTSVMLELTRMLSQSATSPKRTVLFVSFDGEERGLLGSTLYVSHPAFPLDRTVA
                     MINLDHLGAGDGTLTVGVTRMEKTLAQQAADRAGLGDNIQMYGYFPGGDHVPFFEAGV
                     PTVTVVSSGRHPHFHQPSDTLESIQPANLAIATKFLFSLITLLADKPQAACHPQLTPK
                     DLCPG"
     gene            complement(3349..3843)
                     /locus_tag="VLA60_07330"
     CDS             complement(3349..3843)
                     /locus_tag="VLA60_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF09209.1"
                     /translation="MKNLSPEKVADFIHAIVEADRHVYTTHIVKRMQEQGVVMAREDW
                     ENKNAIPLPAQFLHISSKLVAESGHGIRFRLISLWPIYRRNGPATDFERKALEQLSLN
                     SDTPQRGIVSTGKKRLFQAIYADTAINDTCTTCHNAHPLSPKRDFKKGDLMGAIVITI
                     PLED"
     gene            complement(3948..6419)
                     /locus_tag="VLA60_07335"
     CDS             complement(3948..6419)
                     /locus_tag="VLA60_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09210.1"
                     /translation="MDNPEPKPAKRKKGLTKKLVLSMLLVGALPLIIGLLLAFYQGTQ
                     EIREVNGSSFEALATETARKLDLVMADELARTALLTTDVKIIARLEHIRDALSELNPQ
                     ALARTLKQEQESWNAKNAEMLQRILKGPFAEILQQHVGGTFMDPGHPIPLITRSATRG
                     LFITDRAGRVIASLDSGIPYLHREEVWWQGAFHNGVGQPYIGQVAFDSRLGVYTFTIA
                     LPIMDSLRYEAIGVLHRIYDAKEFFAPSIDIIRFGKTGHVMLIDSRGVVLSCPILPTG
                     TSITDTRLIPLVTPLQAGWTPAPSDGHGGTAASIIGFAPLSNTNQITKFSTGQTWHMF
                     VWQSSGEVFAPIEHFFKWTAAFGLLGVALLVTLGYIAANRIAAPIRRLQEAARQIGRG
                     EYREPITLSTGDEIEELADEVNRMNQQLASQFAGLESQVELKTQEVKYLEESTIKILD
                     NVPDPVLMIGPDEHIEYLNKASKEVFALTNGEIIGTPLFQIFSLDPATRDRLKQEMHA
                     VQVMEPPSSDNTQSSPSTTSLQDPLVPTNWAQSGTNRQEIRINQRTFRYRWFGVQARP
                     GRGPSAGLVLRDTTEESILQDRVIQGEKLASLGILSAGIGHELNNPLVGVIGLGEAIQ
                     EETNPEQIKEYARGIVQHGKRMASVVRDFTGQSGKNLTEGQTLINLNELLEQSVTTVK
                     DLFPSVQIEIETHLGPIPSIQGKALELGQAFTNIITNAFQAMKEGGKLAISTEASGHD
                     LEIRIKDTGRGIAPTHLPRVFDPFFTTRGQGEGSGLGLTVAYRIINKLGGHIRMESEE
                     NRGTTCLITLPVSNAHLAKERSSTS"
     gene            complement(6406..7467)
                     /locus_tag="VLA60_07340"
     CDS             complement(6406..7467)
                     /locus_tag="VLA60_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09211.1"
                     /translation="MSTVEPPITVAILGAGKGGTAMLESFLNLPHIRLLGIADTNPHA
                     LGFRLARLHNIPTVSHPLKLIQEDKVNLIIDVTGDPILPSYITEHKHSGAEVLGGTAA
                     KVLLELIQHQTLIQTQLFQSEKLASLGTFASGIAHDINNPLYIILAMAEEIQEENQLE
                     RIRDHAASIHDAAQRIQAISRNITDYVHTPSYQELESIDLHARLEEALSISRFATKFR
                     EITVQKHYQNGLAIQGRSEEILQVFINLITNAIHAMEEKGSLTISTSHRNGTVQIAIS
                     DTGCGISPEHLPKIFNPFFSTKPKGQGTGLGLYNVKTIVKKYHGDLQVESELGKGTTF
                     RLSFPAIPPVEGTVSGGQP"
     gene            complement(7676..8107)
                     /locus_tag="VLA60_07345"
     CDS             complement(7676..8107)
                     /locus_tag="VLA60_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09212.1"
                     /translation="MAETTAAMFGGHKTNGLVLVVDDEPDVRKVVRMTLEKAGYDVIE
                     AEDGEKAIEAIHQGENPLMLDVIISDIRMPKINGVEAINYFQQQWPRVPLIVLTGFPD
                     MEMATGFLNKGIVDYLVKPVEKEKLIKSVATAMEKRDLNRL"
     gene            complement(8590..9756)
                     /locus_tag="VLA60_07350"
     CDS             complement(8590..9756)
                     /locus_tag="VLA60_07350"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF09213.1"
                     /translation="MFQDFWQEILRVWQIGILHTTLGDIFIALGVFLAFLFVRRIFFR
                     FFSRLLQRLTGQTKTDIDDRILEAIERPLEFTFVIIGLYISGQVISLSPELGAIFGQI
                     IRSLIAFTIFWAIFRILDPLSALLDRSITLFGSQSMHETVKGFFLKVAKFVVVCLGIA
                     AVFQEWGFNVAAVLGSLGLVGMAVALGAQDFIKNMFAGFTIFLDRVFEKGNWIKTPDV
                     EGTVEEIGFRATKVRQFDKALVTLPNSKLANEALINYSRMTNRRIYWKIGIEYRSTHD
                     QIRNIIQAISTYIYECGDFETNPEQTKTFIFLDSFGNSSIDIMLYCFTKTIVWGEWLE
                     AKERLAYKIKEIVEGEKASFAFPSSSIYVESLPLGTPESFMPPRSRSPSFPSPT"
     gene            10133..10519
                     /locus_tag="VLA60_07355"
     CDS             10133..10519
                     /locus_tag="VLA60_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09214.1"
                     /translation="MASGHQGWMSVTLATMGLMGCVGQQEFVDAQQAAIGRGIVEGTK
                     GYQQIQQEALSPNRHLRKVQGQVFKIEGAAYVVHVNDQIEARLPFDENTRIDRPAHVG
                     DWIEAQLDRSGRARIIRNIDGQISLE"
     gene            complement(10698..>11632)
                     /locus_tag="VLA60_07360"
     CDS             complement(10698..>11632)
                     /locus_tag="VLA60_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09215.1"
                     /translation="ITEELPSISLGTHCTLRTVETVLYDFGGIMVTYQIPLAGPFASL
                     LPLSEALYENPQLLEGTFRHVQSLLTTLGSAIEKPNISEFVEDYAIFHIFSTQPALNL
                     SQFVNQHGTLLSQILRCEDQPLSEQEIRDATTSQIAFGTQDLTLIDWNAALMIGQEMD
                     DVLAVLEFANVELVERRFLDHQLDEALDEAYGALTQETRHGFRWPGSSDANLRRIARM
                     QVDSAILFERVTNTLKLLGDQYLARVSRLASQRFHLQSWDASILRKLNTLDNLYDKMA
                     GQTGNQRMEILEWIIIILIAISILLPFIPGFPGY"
BASE COUNT         2914 a         2599 c         3110 g         3009 t
ORIGIN      
        1 aattgataaa ttacatatat ccgtgtggta aagtattttg attttattca aggttattca
       61 cttgttgggt atgtctgggt aggtcactga aagagagcca ggtggtagat atgatggtga
      121 agtcgcttat ctatgtggtt gatcccatgt gttcgtggtg ctggggattt tcaccagtca
      181 ttgatgagat cgttcggcga tatcgagatc gggtcacgat cgaggtattg ttgggtgggt
      241 tgcgccccgg caatactgaa cggtttgatg agcgacggcg tgcctatatt ctcagccatt
      301 ggcatgcggt ccatgaacga accggtcagc catttaactt taattttcat cctgaacctt
      361 cgttcatgta tgacactgaa ccgccatcac gagcgatgat ggtaatccgg caattagctc
      421 cggataagga atttgtgttt ttacatgctg tgcaggaagc attctacgta aagaaccagg
      481 atgtcaccca tccggagata ttggcggatc ttgccggcac acaaggtatt gaccaggaca
      541 ggtttctgga atggtttcat gattctgcga tgaaacaatc ggtctggcag gagtttgatc
      601 gggctcgcca attgggcgtg agtggttttc cagccctact tggtcaaaat ggtaatggcc
      661 acatcagatt gacccagggg tatcaaccca caggtgtaat ggtttcactc attgacaaat
      721 ggctggatgg tccagcttcc ggcaacggca gtgggtcgat cggatagatg attatctcag
      781 tataggatgt tggatgattc gtagatcatg tgctcttgtt tcttcatgaa cgaagaccat
      841 gcaaccagta ggtcttcaat ttttacagga ggttatgtga tgcggaaggt ttccaaagtg
      901 tgctttggga ttggaacggc tctgatcgtt ggttttgggt ttgtcacggc atcggttgca
      961 gaaatggcta aatggaaact ggatcatgat cattccaggg ttggatttca agttgcccat
     1021 atggtggtgt ccaaaaccaa tggcaagttt acagagtatt caggaatggt ggagatggac
     1081 gcagaggaga aggaatttaa aaccattgaa gcggtcatcc agaccgcctc ggtcacgact
     1141 gaccatcaga aacgggacga gcatttacga agcccggatt tttttgacgt tcagaagttt
     1201 ccgaccatga cctataccat gaaaagttat aagaaaaccg gagatgagta taccgctaca
     1261 ggagatttga ccttgttggg agtcactaga gagatcacat tagtcggaac gtttaatggg
     1321 gtggctcagg atccttgggg caatacacgg gctggcttta ccgcctccgg cacgctgaac
     1381 cgaaaggatt tcggcatgaa attcagcaaa ttattagata gtggcggaat gctcgtgggt
     1441 gatgaggtca acatcattct ggaaattgaa gtaatcaaag aaaagacagt cgaaaaagtg
     1501 aaacaagaaa aagaataaag tgataggggg gaatgtgtgg taaccgtctg catgtttcct
     1561 gtgagaatta acgaggaaat ctgagcagtg aaggaataga ggagaaggaa ggttcggtgc
     1621 cgccatattt tatggtaagc caatcggcaa gctcagaaca tatttcccaa aggatttgac
     1681 aaattccaga aaccttcaac cggggcacag atccttcggg gtaagctgag gatgacaggc
     1741 tgcttgaggc ttgtcagcta acagggtaat cagtgagaag agaaatttgg tagcgatggc
     1801 caaatttgca ggttgaatgg actccagggt atcagatggt tgatgaaaat gggggtgcct
     1861 gccgctgctg acaactgtca cggtcggcac cccggcttca aaaaatggga catgatcgcc
     1921 cccggggaaa tacccataca tttgaatatt gtcgcctaat cccgcccggt cggctgcttg
     1981 ctgagccagc gtcttttcca ttcgagtaac tccaaccgtc agtgttccat cccccgctcc
     2041 tagatgatca agattgatca tggcgaccgt tcgatccaat ggaaaggcgg gatgactgac
     2101 atagagcgtc gatcccagta ggcctcgttc ttctccgtca aaagacacga agagcacggt
     2161 ccgtttgggt gaggtggcgc tttgggagag cattctggtc agttccaaca tcactgatgt
     2221 tccggatgca ttgtcatcgg ctccaggaaa aagcaagccg gcttgctggc caaagtggtc
     2281 gcgatgcgca ccgatgagaa tgagttcttt gcgtagctcc tgatcgcgac ctggtaacat
     2341 gccgatgaca ttggtcagtg ttccgggctg actgtggctt tcccaatgaa attggaccag
     2401 aaggggaagg gggctgctac gaaaggtccc gatatcgttg gcggcctgtt gcagggcttc
     2461 gagcgagtca ttggctgcat tcaaggcctg gtcaagaagt ttcccatgga tccatgcccc
     2521 agggatgggg cggttatcgg gtgtttcccc atagatggcc aaaggaccct gccccagacc
     2581 tttgcgggct tcataacggc caagtagtgg accggttacc gtcaagtagc ctaccgcccc
     2641 tctttctttg gcgatggccg ctttttcttc atgcgtgatc caccccgggt aggaaggggg
     2701 ttttccccgg agaaatagga cgatgcgatt gtcgacattc aggccctgat attcatctat
     2761 tccgcgggca gggtcgacaa tgccgtatcc cacaaagacg actctggctg tggttctggt
     2821 ggaaggagaa tccaaaattg gaagaaactc ctgacctgga acaagtgaca tcgtcattga
     2881 cgtccggttc gcgccgataa gggaaagggt gagggtcgat ggctccagca attgaaccgc
     2941 ggtcatgaac cgttgttgca accaatgaaa atcctgttcg tgttttgaaa cgtggttggc
     3001 cggttgtaag ccgagagatc ggaatcgatc aaccagataa tcggccgatt gcaagctacc
     3061 atccgtgccg gcctgccgac cttgatacga tggatgactc agttcgcgaa tgtcctggat
     3121 gatatttcca ataaccatgg gcatggatgg agaagaggag ggctgagggt caaagcccaa
     3181 aacaggagtc gtgagggcga gtcctatcat aaattggaca atgggaaatc gtaacaagtt
     3241 tcttacctat tccttgtcat gacggggatt gaaagaatgc gtgtaacagg ggaagggtat
     3301 aatcgaatgt gtgaaaaatg gagcctgtgg cggaacctgt gccgggcgtt agtcctccag
     3361 aggaatggtg atgacaatgg cccccatgag gtcgcccttt ttgaagtcac gtttggggct
     3421 taaggggtga gcattatggc aagtcgtgca ggtatcgtta atcgctgtat cggcataaat
     3481 ggcctggaac aagcgttttt ttccggtgga tacaattcca cgttgaggcg tatcggaatt
     3541 gagcgaaagt tgttccaaag cctttcgttc aaaatccgtt gcaggcccat tccttcgata
     3601 aattggccac agactaatta aacggaaacg aatgccgtga cctgattccg cgaccaattt
     3661 actggaaata tggagaaact gcgcaggaag gggaatggca tttttgtttt cccaatcttc
     3721 tcgcgccatg acgacgcctt gctcctgcat tcttttcacg atatgtgtcg tgtagacgtg
     3781 acggtccgcc tcaacgatgg catgaataaa gtctgcaact ttctccggtg atagattttt
     3841 catggcgttg gattcacctg cttgaagaga tgaagaaaca cctattccta tgaagagagt
     3901 gccaagaaaa accatagata agccggtcct actacctcct aggcttgtca tgacgtcgaa
     3961 ctcctttcct tcgccaggtg ggcattcgag acgggtaagg tgatcaaaca ggtggtcccc
     4021 cggttttctt cactttccat tcgaatgtgt ccgcctaatt tattgataat tcgataggcc
     4081 acggtgagtc caaggcccga tccttctccc tgccccctcg tggtaaaaaa agggtcaaag
     4141 actctgggca gatgagtcgg ggcgatgccg cgccctgtgt ctttaatgcg gatttccaga
     4201 tcgtgtcctg acgcttctgt tgaaatagca agcttcccac cttctttcat tgcttgaaaa
     4261 gcattggtga tgatattggt aaaggcctgt ccaagttcca aggcttttcc ctgaatggaa
     4321 ggaatgggtc caaggtgagt ctctatttca atctggactg atggaaagag atccttgacc
     4381 gtagtcacgg attgttccaa cagttcattg aggttaatga gcgtttgccc ttcagtcaaa
     4441 ttttttccgg attgacccgt aaagtcccga acgacggaag ccatacgttt tccatgttga
     4501 acgatgcccc tggcatattc tttgatttgc tcggggttgg tctcctcttg tatcgcttca
     4561 cctaatccaa tgactccgac taaaggatta ttgagctcat gtccaatccc ggcactgaga
     4621 atgcccaaac ttgcaagctt ttccccctgg ataacccggt cctgaaggat actttcttcc
     4681 gtcgtatctc gtaataccaa tccggcactt ggtcccctcc ccgggcgggc ctggacacca
     4741 aaccaacggt accggaacgt tcgctgattg atccgaattt cctggcgatt ggtgcccgat
     4801 tgagcccaat ttgtgggaac cagaggatct tgcaacgagg tggtggacgg actggactgc
     4861 gtattgtctg aagatggagg ttccataacc tggaccgcgt gcatttcttg tttcaggcgg
     4921 tctcgtgtcg caggatccaa ggagaaaatt tgaaagagtg gcgtgccgat gatctcgccg
     4981 ttcgtgaggg cgaagacttc tttcgaggcc ttattcaaat attcgatgtg ttcgtctggg
     5041 ccaatcataa ggaccgggtc aggcacatta tccagaattt taatggttga ctcctcaagg
     5101 tatttcactt cctgggtttt caactcaact tggctttcca atccggcgaa ctgtgaagcc
     5161 agttgttggt tcatgcgatt gacttcatcg gccaattcct caatttcatc gccggtggat
     5221 aatgttatgg gctcccgata ttcccctctg ccgatttgcc tggcagcttc ctgaaggcga
     5281 cgaatgggtg cggcaattcg attggccgct atatagccca atgtgacgag cagcgccact
     5341 ccgagtaatc caaaagctgc cgtccattta aaaaaatgct caatgggggc aaagacctca
     5401 ccggatgact gccagacaaa catatgccag gtctggccgg tggagaattt ggtaatctga
     5461 ttggtattac tcaggggggc gaatccaatg atggaggctg cagtgccccc atggccgtcg
     5521 cttggggctg gagtccatcc ggcctgaagg ggggtgacga ggggaataag ccgggtgtca
     5581 gtaattgagg taccggtggg aaggatcggg cagctcaaga cgacgccacg gctgtcaatg
     5641 agcatgacat ggccggtttt gccaaatcga ataatgtcga tggaaggggc aaagaactcc
     5701 ttggcgtcat aaatccggtg caggacgccg atggcttcat accgcaaact atccataatg
     5761 gggagcgcta tagtaaacgt atagactccc agtcgtgaat caaacgctac ctggcctata
     5821 tacggttgac cgactccatt gtggaatgca ccttgccacc atacttcttc ccgatgaagg
     5881 tagggaatgc cggaatccag actcgcaatg acccgtcctg cacgatcggt gataaataag
     5941 ccacgtgtgg cagaccgggt aatcagcgga atgggatggc ccgggtccat aaaggttccc
     6001 cccacatgtt gttgcaggat ctcggcaaat gggcctttca gaattcgttg aagcatctcg
     6061 gcattcttgg cgttccagga ttcttgttct tgtttgaggg tgcgagccag cgcttgggga
     6121 ttcaactcac tgagtgcatc ccggatgtgt tccaggcgtg cgatgatttt gacatccgtt
     6181 gtcagtaagg ccgtgcgagc cagttcatct gccatgacca gatcaagttt tctggctgtc
     6241 tctgttgcga gagcctcaaa gctggatcca ttgacctcgc gaatttcctg tgttccctgg
     6301 tagaaagcca gcaggagtcc aatgatcaag gggagtgcgc cgaccaacag catggacaga
     6361 accagtttct tggtcagtcc tttttttctt ttggccggtt tgggttcagg gttgtccacc
     6421 ggacaccgta ccttctacag gagggatggc ggggaaggac aatcggaagg tggtcccttt
     6481 ccccaattcg ctttcgactt gaaggtcccc atgatatttt ttgacaatgg ttttgacgtt
     6541 atagagtccg aggcccgtcc cctggccttt cggtttcgtt gaaaagaagg gattgaaaat
     6601 ctttgggagg tgttccgggg aaatgccaca accggtatca ctgatcgcaa tttgaacggt
     6661 tccgttcctg tgtgaggtgg aaatggtgag gctgcctttc tcttccatgg catgaatcgc
     6721 gttggtaatc aaattgataa atacctgaag gatttcttcg gatcttccct gaatagccaa
     6781 gccgttttga taatgcttct gaacggtaat ttcccgaaat tttgtcgcaa acctggaaat
     6841 gcttaaggcc tcctccagtc tcgcatggag gtcgatgctt tcgagttcct gataactggg
     6901 tgtgtggaca tagtccgtga tatttcgtga aatagcttga atgcgttggg ccgcatcatg
     6961 gatgctggcg gcatggtctc ggatgcgttc gagttgattt tcctcttgaa tttcttcggc
     7021 cattgccaaa atgatataca gagggttatt aatgtcatgg gcaattcctg aggcaaaggt
     7081 tcctaagctg gctaattttt ccgattgaaa taattgtgtt tggattaagg tttgatgttg
     7141 gatcaactct aggagaactt tggcggccgt ccctccgagc acttcagccc cgctgtgttt
     7201 gtgctccgta atatatgaag gaagaatggg atccccggtc acgtcaatga tcaaattcac
     7261 cttgtcttcc tgaataagct tcagaggatg agagactgtc gggatattgt gaagtctggc
     7321 aagtcgaaaa cccaacgcgt ggggatttgt atcggcaatg cccaaaagac ggatgtgggg
     7381 taaattaaga aaggattcca acatggccgt tccccccttg ccggccccga gaatggcaac
     7441 ggtaattgga ggttcaacgg ttgacataga atccttcctt gattgttgga gcggtgaagg
     7501 atggaaggct aaggccaggt ggggagtatg agatgtttct ccatgtctca acattcatat
     7561 ggtagaaagc cctatccaga ggatagggct ttactcgata taccatagtc ttcttcatgg
     7621 ggaaggattc tcccgcgaga agaaactcga aatctgaaaa gttcgttctg tcagattaca
     7681 ggcgattgag atctcgtttt tccatggcgg tggccacgga tttaatgagc ttttcttttt
     7741 caaccggctt gaccagataa tccacaatcc ccttattgag gaatcctgtg gccatttcca
     7801 tgtccggaaa tcctgtgagg acaatgaggg gaacacgtgg ccattgttgt tggaaatagt
     7861 tgatggcttc cacgccattg atttttggca ttcgaatatc ggaaataata acgtccaaca
     7921 ttaaggggtt ttcaccttga tggattgcct cgatagcctt ttcgccgtct tcggcctcga
     7981 tgacatcata tcctgccttt tcgagggtca ttcgcacaac ttttcggaca tcgggctcat
     8041 cgtccaccac gagaaccagt ccgtttgttt tgtgtcctcc aaacattgct gcagttgttt
     8101 ccgccattac tcttctcctt tgttgttagg tgactagcct gattggattt ttaattggtc
     8161 agagggaaaa gaaaaatgtt cttatctctt ctgaaggcac aatattatcg taatcaagca
     8221 ataagagaaa gggttaattt ggccttaata ttgagcctat tgggaatgac aaaggcggat
     8281 atagggtaac tcgtccgggc gtgcccaata ttgcttctga agcctgcctg gaaatagtcc
     8341 tgttttcctc cctcctgttc atttcggtgt ctcaagccgg atcagtcaac cttttagttg
     8401 caggccccct cgaaaaaagg cagagtggct aggtgagaag ggtctggatt gaatcgtcgg
     8461 catttttcac acgaagcgaa cgggtaggta aagcgtcaaa ttctctggca tgcaaggagt
     8521 ggtttttctt gggaaaacag aacgctcttc cagcttggca gcctacgccc acccatagaa
     8581 acacggaaat tatgttggcg atggaaaaga aggcgaccgg gagcggggag gcatgaaaga
     8641 ttctggtgtg ccgagtggca gactttcgac ataaatggaa ctcgatggaa acgcaaagct
     8701 ggccttctcg ccctcgacga tttccttgat tttgtaagcc aatcgttctt tcgcttccaa
     8761 ccactcaccc catacaatgg tcttcgtaaa acaatacagc ataatgtcga tactcgagtt
     8821 gccgaatgaa tccaggaaaa tgaacgtttt ggtctgttcg ggattggttt caaagtcacc
     8881 acattcgtaa atgtaggttg aaatggcttg gataatattc cgaatttgat catgagtcga
     8941 acgatactcg atcccgattt tccaataaat tctccggttc gtcatgcggg agtaattgat
     9001 taacgcttca ttggccaatt tggaattggg cagtgtgacc agggctttat caaattgacg
     9061 gacttttgtc gcacgaaatc caatttcctc caccgtgcct tccacgtcag gcgttttgat
     9121 ccagttgcct ttttcaaaga ccctatccaa aaatatcgta aatcccgcaa acatattctt
     9181 aatgaaatcc tgggcgccga gggcgaccgc catgccgacc agtcccaagc tcccgaggac
     9241 cgccgcgaca ttgaatcccc attcctgaaa caccgctgcg atgcctaagc agaccaccac
     9301 gaactttgcg accttgagga aaaacccctt aacggtttcg tgcatgcttt ggctgccgaa
     9361 gagtgtgatg gatcgatcca gtagcgctga cagcggatcc agtattcgaa aaatagccca
     9421 gaagatcgtg aaggcaatca aggaccggat gatttgtcca aaaatcgcac ccaactcggg
     9481 tgacaaggag atgacctgtc cggaaatgta cagaccaata atgacaaacg tgaattcaag
     9541 cgggcgttca atggcttcca ggatgcgatc gtctatgtcc gttttggttt gtccggtcag
     9601 ccgttgcaag agacgggaaa aaaaacggaa gaaaattcga cggacaaaca agaatgccag
     9661 aaatactcct aaggcaatga agatatcacc cagggtggta tgtaaaatgc cgatctgcca
     9721 gaccctaaga atttcttgcc aaaaatcttg aaacatcaat cgaaggttcc tgacctcaat
     9781 gaaaagattg aagaggggtt aaatgaaggt atatgttaat tagcaggatg aagccgggcg
     9841 cgtctctgaa tgacattgct ggacggatcg atgcatgcgg atcaatcatg ttgtgaaaat
     9901 gttcacggga agggtaaaag aattctatgt aaaggtcaat tttcattgtg gaattggaga
     9961 gaaatatcgt gggtatgaaa gagagaagtg agctattgga agcggagttt ctcgcgtcac
    10021 actgtctggg atgagcccta tttcctagac tctctgagcg cgtgtgatag gataaattgt
    10081 attggcaccg tttatgacct acctgagtga gtaggagaaa ggaaagccgg gaatggcttc
    10141 aggtcatcag ggctggatga gcgtgacatt ggccaccatg ggactgatgg gatgtgtggg
    10201 acagcaggaa ttcgtggacg ctcagcaagc ggctatcggg cgtggcattg tcgaaggcac
    10261 caaaggctac caacagattc aacaagaagc gttgtccccc aataggcatc taagaaaggt
    10321 tcaggggcag gtgtttaaaa tcgaaggcgc ggcctatgtc gtgcacgtaa acgatcagat
    10381 tgaagctcga ctgccttttg atgaaaatac ccggattgat cgtccggcgc atgtggggga
    10441 ctggattgaa gcgcagctcg atcggtccgg acgcgccaga attatccgga atattgacgg
    10501 ccaaatttca ctcgaataag cgccgttctt tttcaatcct gaccctcttc ggtttctcgt
    10561 tgaggcgtcc caataatccg ctctcagaag tcttgtcttg caactctttt caactgagaa
    10621 tgttgagtca tattatgatg cacatgagag ttgtttggga gtgatggcgg tgtgaaaccc
    10681 tgagttaaag gacgcagcta atatccgggg aagccgggga taaaaggaag gagaatggag
    10741 attgcaatga gaataatgat gatccattca agaatttcca tacgctgatt gccggtttga
    10801 ccggccattt tgtcatacaa attatccagt gtgttcaact tgcgaagaat gctggcatcc
    10861 cagctttgca aatgaaagcg ctgagacgcc agccgcgaga ctcttgccag atattgatcg
    10921 cccaataatt tcagggtatt ggtcacccgt tcaaagagaa tggcgctatc gacttgcatg
    10981 cgtgcgattc ggcgaagatt cgcatctgat gatcccggcc aacgaaatcc atgccgggtt
    11041 tcctgtgtaa gagcgccata cgcttcgtca agcgcctcgt ctaattggtg atcgagaaat
    11101 cgccgctcga ctaattccac attggcaaat tctaggaccg ccagcacgtc atccatctct
    11161 tgcccgatca tcagcgcggc attccaatca atgagggtca gatcctgcgt tccaaaggca
    11221 atttgactcg tggtggcatc ccgaatttcc tgttctgaca acggttgatc ctcacaacgg
    11281 aggatttgag acagaagggt gccgtgttgg ttcacaaatt gcgacagatt taacgcgggt
    11341 tgagtcgaga aaatatgaaa aatcgcatag tcttccacaa attcggatat attcggtttt
    11401 tcaatggctg aacctaacgt ggtcagaaga gactggacgt ggcggaatgt gccttcaagc
    11461 agttgagggt tttcgtagag tgcttcactt aacgggagca gggacgcaaa tggtccggcc
    11521 agaggaatct ggtaggtgac cataatcccg ccgaagtcat agaggacggt ttcgaccgtg
    11581 cgaagggtgc agtgggttcc gagactgatc gatggaagtt cttccgtaat gc
//
LOCUS       DATIAL010000237         3625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1441804, whole genome shotgun sequence.
ACCESSION   DATIAL010000237 DATIAL010000000
VERSION     DATIAL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441804"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VLA60_07365"
     CDS             <1..485
                     /locus_tag="VLA60_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09216.1"
                     /translation="IAMALACQPDLLIADEPTTALDVTIQAQILDLLSALQKDMGMAI
                     LLITHNLGVVAQFAQDVIVMYASKIAERATVKELFRNPSHPYTSALLKSLPRPGERQA
                     RLEAIPGTVPSPLQYPVGCHFSSRCPDVLEYCPSQPPPTVQVGESHETVCWLYDKKEA
                     "
     gene            558..1544
                     /locus_tag="VLA60_07370"
     CDS             558..1544
                     /locus_tag="VLA60_07370"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HSF09217.1"
                     /translation="MTESTTSLLQVTGLKKYFPVRSGIFSRVSAWVKAVDDVTFHMNQ
                     GETLGLVGESGCGKTTVGRSILRLMEPTAGTVTFEGRDVLALSSKELRQTRRRMQIIF
                     QDPYSSLNPRMTIGAIVAEPLKIHQIAKGQDLQDQVNQLFVRVGLRPEHQSRYPHEFS
                     GGQRQRVGIARALALNPKFIVCDEAVSALDVSIQAQILNLLRDLQQEFHLSYLFITHD
                     LNVVQYLADRIAVMYVGKFAEVAPAEDLFATPKHPYTQALLSANPVHDPTAPSKRIIL
                     PGDVPSPLNPPAGCRFHPRCPEVMDVCKTVEPKLTQIGPPEKGHQVWCHLYP"
     gene            1925..2110
                     /locus_tag="VLA60_07375"
     CDS             1925..2110
                     /locus_tag="VLA60_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09218.1"
                     /translation="MSNVKEKMTEVIQSQPEDATYEEIMRELAFERMIERGLVDSRRG
                     RVISNEDMERRIRTWQK"
     gene            2098..2394
                     /locus_tag="VLA60_07380"
     CDS             2098..2394
                     /locus_tag="VLA60_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSF09219.1"
                     /translation="MAEIRWTEEAHRWLYDIFEYIAADNPEAAQKVVSGIYEKAQVLR
                     RFPEIGHKYRTETDGEIRILLYGHYRIAYLLMSSSSIDILGVFHGALDIDRYLP"
     gene            complement(2733..>3625)
                     /locus_tag="VLA60_07385"
     CDS             complement(2733..>3625)
                     /locus_tag="VLA60_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HSF09220.1"
                     /translation="GVNGVEIWKSDGTESGTVLVKNIRPGANGSAPEQLINVNGTLFF
                     VAGDGTNGLWKSDGTEGGTIKIATFNFYPEWLTNVNGTLFFSAFHSSYGGVELWKSDG
                     TIAGTELVRNLNGVSNSWPRWLTNVNGTLFFTAVDFIPSTGSFNGLFKSDGTSAGTVL
                     VKEVSSAVDLTRVGNKLYFRALTPSVGYQPYISDGTTAGTRALEADLFLNSLASEGPW
                     FTDVNGIAFFSANDGITGTELWKSDGTEAGTVLVKDIFPGGNSAPQSLTNVDGTLFFI
                     AGSSPGSPGVGRELWLACGM"
BASE COUNT          875 a          965 c          885 g          900 t
ORIGIN      
        1 tgatcgccat ggccctggcg tgtcaacctg atctgttgat cgccgacgaa ccgactacag
       61 ccctggatgt gaccatacaa gcgcaaattc tggatctgct gtcggctctc caaaaggata
      121 tgggcatggc tattctcctg attacgcata acttgggggt ggtcgcgcaa tttgcccagg
      181 atgtcatcgt catgtatgcg agtaagattg ccgaacgggc aaccgtgaaa gaattattcc
      241 ggaatcccag tcatccctac acgagcgcgc tcttgaaatc gctgcctcga ccaggggagc
      301 gtcaggccag gctggaagcg attcccggaa ccgtgccgtc tcccttacag tatccggtag
      361 gatgtcattt ttcctcccgc tgtccggatg tgcttgagta ttgtccgagt cagcccccac
      421 ccacggtgca agtaggagag tcgcatgaaa ccgtgtgctg gttatatgac aaaaaagagg
      481 catgagagag cagatcgtca tgtccaattc tgtggtaaaa agatcctttg tccgactgta
      541 agatttcgtt gttactgatg actgaatcca cgacatcatt actccaagtc acaggattga
      601 aaaaatattt tcctgttcgg agcgggatct tttcccgtgt gtcggcatgg gtcaaggcgg
      661 tcgacgatgt tacctttcac atgaatcagg gagagacgtt gggcctggtc ggggagtcgg
      721 gttgtggaaa aaccacggtg ggacgaagca ttctccgact catggagccc actgcgggaa
      781 cggtaacctt cgagggaagg gatgtgttgg cactctcttc gaaggagtta cggcagacac
      841 gtcgccggat gcaaatcatt ttccaagacc cgtatagctc actcaatcct cgcatgacca
      901 tcggggccat tgtggcggag ccgctcaaga ttcaccagat cgccaaaggc caggacctgc
      961 aggatcaagt gaatcagttg tttgttcgtg tggggttgcg gccggagcat cagtcacggt
     1021 atccgcatga attctcaggc ggccaacgcc agcgtgtcgg cattgcgcgt gcacttgcgc
     1081 tcaatccaaa attcatcgtc tgtgatgaag ccgtgtcggc gctggatgtg tccattcaag
     1141 cacaaatcct gaacctgctt cgcgatctcc agcaggaatt tcacctttcc tatttgttca
     1201 tcacacatga tctcaatgtg gtgcaatatc ttgcagaccg gatcgcggtc atgtatgtgg
     1261 gcaagttcgc ggaagtcgct cctgctgagg atctctttgc cactcccaag catccctaca
     1321 cacaggccct gctctccgcc aatccggttc acgatccaac agcgccctcc aaacgcatta
     1381 tccttcccgg cgatgttccc tcgccgttaa atcctcccgc cggctgccga ttccatcccc
     1441 gctgccccga agtcatggat gtctgcaaaa cagtagaacc aaagttgacg cagatcggtc
     1501 ctcccgaaaa aggccaccag gtctggtgtc acctgtatcc ttaatcaacc tccggctcaa
     1561 taattttcag tctactcaac ctctggattt gaaaaaatta atttctctgt tcagccattg
     1621 cacgaacgcc agatgattta gtgtgccctt actcgccggg tcatcctgag ccaccggcga
     1681 aggatctgtg tccaggtaaa atagtccgga ttatcttttt cgtcattacc gacgttatta
     1741 atcgggagtc tatcttagtt tcttttggat ggatccatat ttcacgtcga ccctgtgctg
     1801 tggatagtgt tgacaattct ataaatcaag acgacaatca gtcagaagtt tgaggagagg
     1861 gttcgtatgc tatgccggtc agtatgatca aaaggcctgt acctctgtta aaggggagtg
     1921 tgatatgtca aacgttaaag aaaaaatgac cgaagtaatt cagtctcaac ctgaagatgc
     1981 gacgtatgag gaaatcatgc gggaactcgc ctttgagagg atgatcgagc gcggtcttgt
     2041 ggattctcgc agggggcgag ttatatccaa cgaagacatg gagcgccgta ttcgcacatg
     2101 gcagaaataa ggtggactga ggaagctcat cgctggcttt atgatatatt cgaatacatt
     2161 gccgcagata atccagaagc agcccagaaa gtcgtctctg gaatttacga aaaagcccag
     2221 gttctacggc gtttccccga gattggccac aagtatcgca ccgagaccga tggtgaaatc
     2281 agaatcttgc tttacggaca ttaccggatt gcctaccttc tgatgtcctc atccagcatt
     2341 gatattttgg gagtatttca cggggcactc gatatagaca gatatcttcc gtgattcatg
     2401 cgaatgccct atcattccac tgcgttgctc tacgtccgca ttttctgcct cgaatggaaa
     2461 acggtggaac ctcagttaat tcaagtcgac cctcccgaac gccgccagta ggtctggtgc
     2521 cacttgtatg cttgattcag agaattcctg atcagcagtc tcagtcaact caatccgatc
     2581 aaaagtcatc ttgagcccac ggcgaagtat ctgtacgcag gtaaagaagc cagggggcag
     2641 caggatcttt cgtggaactt gtcccggctg cattaagtgc ttataccgtg agcatgatac
     2701 cgaagtggga aaatgcccat tcctcctcac tgctacatcc cacaggccaa ccacagttca
     2761 cgcccgaccc ctggagatcc tggagaagaa cctgcaatga agaagagtgt cccatccaca
     2821 ttggtgagac tctgcggcgc agaattcccc ccagggaaaa tatccttcac cagcactgtt
     2881 cctgcctcgg tcccgtcgct cttccaaagt tctgtgccgg tgattccgtc gttagcactg
     2941 aaaaatgcta tcccattcac atccgtgaac cacggcccct ctgaagcaag actgtttagg
     3001 aaaagatcgg cttccagcgc ccttgtccca gccgtagtcc cgtcactgat atacggttga
     3061 tagcctactg atggggttaa agctcggaaa tacagtttat tacccacgcg agtgaggtct
     3121 acggcactgc ttacttcctt taccagcact gtaccggcgg atgtgccatc actcttaaag
     3181 agaccattaa aagagccggt gctagggatg aaatccacag cggtgaagaa cagggtccca
     3241 ttcacattgg tcagccaacg aggccatgaa tttgagaccc cgttgagatt tctcaccagc
     3301 tctgttccag ctatggtacc atcgctcttc cagagctcga ctcccccata ggaactgtga
     3361 aaggcggaga agaacaatgt gccatttaca ttcgtcagcc attccggata aaaattgaat
     3421 gtagcgattt tgattgttcc gccctcagtt ccatcgcttt tccaaagacc gtttgttccg
     3481 tctcctgcca cgaaaaacaa tgtcccgttc acattgatga gttgttctgg agcggatcca
     3541 ttcgcaccgg gtcggatgtt cttcaccaac accgttccgg attcggtgcc gtcacttttc
     3601 cagatctcga ccccattcac gccat
//
LOCUS       DATIAL010000238         4972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_795966, whole genome shotgun sequence.
ACCESSION   DATIAL010000238 DATIAL010000000
VERSION     DATIAL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795966"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VLA60_07390"
     CDS             complement(<1..1567)
                     /locus_tag="VLA60_07390"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF09221.1"
                     /translation="MAAHPVVIIGAGPAGLTAAYELGKNSSSSLILEAGKQVGGISQT
                     VNYRDFRFDIGGHRFFSKVPMVTELWNEILGDNFLLRPRISRIHYNQHFFDYPLKATN
                     ALAGLGVVEALLVCFSYAKVKLFPNAREENFEQWVINRFGYRLYQIFFKTYTEKVWGI
                     SCTEISADWAAQRIKNLSLKEAVRNALLGQRGGKKGEIVTSLIEQFHYPRLGPGMMWE
                     RCEELVADFGSQTLKGMKVERIRHRHGRVDCVLARASSGEHLEFEGSDFVSTMPLREL
                     IEAMDPQPPEKVIEAALGLRYRDYLTVVLVVNCEDVFPDNWIYIHSPEVKMGRIQNYK
                     NWSPYMVPDPSRTSLGLEYFLWDKDDEWTWSNERLIEFGTRECAQLGLINPREVEDGT
                     VVRMEKAYPVYDHHYQDHVRTIRQYLETFSNLQTIGRNGLHRYNNQDHSMVTGVYAAR
                     NIMGESHHDVWAVNTEKAYHEEDRTTSGNGGDRMVPVRVQVSGDELPIENEEELIEIV
                     FAKLDPVAMGVAVG"
     gene            complement(1567..2685)
                     /locus_tag="VLA60_07395"
     CDS             complement(1567..2685)
                     /locus_tag="VLA60_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF09222.1"
                     /translation="MNKIDRNDEEQKVSGTLRSSLTEITISIVVPVYNGGEAFRQCLI
                     SLAALQPPPMEIIVVADGDSDGSWQVAKQFGAQVIRLPITSGGPAYPRNIGALQAKGE
                     YLFFVDADVCVHPDTLGRVAETFHNNPDLTALIGSYDDTPAEINFLSQYKNLFHHYVH
                     QKAQKEASTFWGACGAIRRDIFLEIGGFDENYRRPSIEDIELGYRLKNTGHQILLCKD
                     IHVKHLKRWGICSMLKADFFYRAIPWTELILRDRRFTNDLNIRHSERLCVVLTYGLVG
                     ALMGAIWWVPLLAVAALIMVSLVIINAPLYRFFQKKRGFRFALQSVPWHWLYYLYSGL
                     AFAIGLARHVFYGGNRKESTMPALVPESFDINRTSGRQ"
     gene            complement(3115..3191)
                     /locus_tag="VLA60_07400"
     tRNA            complement(3115..3191)
                     /locus_tag="VLA60_07400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3155..3157),aa:Met,seq:cat)
     gene            3279..3914
                     /locus_tag="VLA60_07405"
     CDS             3279..3914
                     /locus_tag="VLA60_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09223.1"
                     /translation="MTFAGIIAILLFLGLIIGDWYQFTTLKSWACQYGCPLAHRKNAL
                     DSQSVSSISTVFQTGALALPHGIARWVADRQVIVIRPYYQLFSLRFRTAWPLKGTIDV
                     SSDGSQLLLHLSKRIPWSSAILTLLWLLFVIGGTLTFVVLFILDGGISTVGGVFWVVG
                     MTALGLLVLAGGLILLSFAYRLEDSRLTQVYLELLDVLTPPLKDLNKSGSP"
     gene            complement(3976..>4972)
                     /locus_tag="VLA60_07410"
     CDS             complement(3976..>4972)
                     /locus_tag="VLA60_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSF09224.1"
                     /translation="EENIWRVKEKSGYRADVEKVKQTLIGLAELRISEPKTKNPELYD
                     RLGLQDKEQEGSLSTTVRLKTPNNPEAALVILGNQRPAKGNPRMSEIYARKPGDPQTW
                     LTIGNLPLEKVAGEWLDKEITALTTKRVHRVTVRHPGGETLLVSKDKPEDLDFQLDSI
                     PAGSKVASQFNVNNVVGTLVQLSLEDVKEGHEINFQDRSGVTAVLETFDGLRLHVQTA
                     KQEDKVFGKFSAEYDQKLLQPVDTTPSTENGETVPAQDSVQDKKAPESPEAKKTAEIQ
                     DELSSKEDSILKKPEEVQREVEAFNQRVGGWAYELPTFRVENFSKAKKDLLETIP"
BASE COUNT         1259 a         1308 c         1090 g         1315 t
ORIGIN      
        1 taccgacagc cacacccata gcgacaggat ccagcttggc aaacacgatc tcgattaact
       61 cttcctcatt ttctatgggg agttcatccc cgctcacctg aacccgtaca ggcaccattc
      121 gatcgccgcc attgccggaa gtcgttcggt cttcctcatg gtaggctttt tcggtgttca
      181 ccgcccatac atcatgatgg gattcaccca tgatatttct cgcggcatac acccccgtca
      241 ccatggaatg atcctgattg ttatagcgat gcagaccatt tcgtccaatg gtctgaagat
      301 ttgaaaatgt ttccagatac tgtcgaatcg tccggacgtg atcctgatag tgatgatcgt
      361 ataccggata ggccttttcc atacgaacca cggtcccgtc ttcaacctcg cgaggattaa
      421 tgagcccgag ttgggcgcat tcccgtgtac cgaattcgat caaccgctcg ttcgaccatg
      481 tccactcatc gtctttgtcc cagagaaaat actcaaggcc cagagaagtc cgtgagggat
      541 ccggcaccat atacgggctc caatttttat aattctgtat tcgtcccatt ttgacttcag
      601 gtgaatggat atagatccag ttatcaggga atacgtcttc acagttaacc accagaacca
      661 ccgttaaata atcccgatac cgcaggccaa gggcggcctc tatcaccttc tcaggtggtt
      721 gaggatccat ggcctcgatt aactcgcgca gaggcatcgt cgacacaaaa tcgctgcctt
      781 cgaattccaa atgttcccct gatgaggctc gcgctaaaac acaatctact cgcccatggc
      841 ggtgtctgat acgctcgact ttcatgcctt ttaatgtctg agatccaaaa tcagccacca
      901 attcctcgca ccgttcccac atcatccccg gacctaagcg aggataatga aactgctcaa
      961 tgagcgaggt cacgatttca cccttcttcc cacccctttg accaagtagg gcatttcgaa
     1021 ccgcttcttt caacgagagg tttttgattc gttgcgcggc ccaatcggct gatatctcgg
     1081 tacaggaaat cccccagact ttttccgtgt aggttttgaa gaatatctgg tacaggcgat
     1141 atccaaaacg attaataacc cattgttcaa aattttcctc ccgggcattt ggaaatagct
     1201 taaccttggc ataactgaaa cagaccagaa gagcctccac gacccccagt ccagccaacg
     1261 cattagtggc cttaagcgga tagtcaaaaa aatgttggtt gtaatggatt cggcttatac
     1321 gaggtcgaag gagaaagttg tcaccgagaa tttcattcca taactctgtc accatcggca
     1381 ctttagaaaa aaatcggtgg ccaccgatat caaatcgaaa gtctcgatag ttgaccgttt
     1441 gtgatatgcc tcctacctgt ttcccggcct caaggataag agaggaagaa ctgtttttcc
     1501 caagttcata ggccgccgta aggcctgcag gaccggcgcc aattatcact acaggatgag
     1561 ctgccactac tgcctccctg acgttctatt gatatcgaag gattcaggga caagagccgg
     1621 cattgtcgac tccttccggt tgcccccata aaaaacatgg cgtgccaagc caatagcaaa
     1681 cgccaaacca ctatacaaat agtacagcca atgccaggga acactctgca gggcaaaccg
     1741 gaacccacgc tttttttgaa agaagcgata gaggggtgca ttaatgatca ccagcgaaac
     1801 catgatcaag gccgccaccg ccagcaatgg aacccaccag atcgctccca tcaaggcccc
     1861 cacgagccca tacgtcaata cgacacaaag gcgctcggaa tgccggatat ttaagtcatt
     1921 ggtaaacctg cgatcccgaa gaattaattc tgtccaaggg attgcgcggt aaaaaaaatc
     1981 tgctttgagc attgagcaga taccccatcg tttcaaatgt ttcacatgga tatctttaca
     2041 caacagaatc tggtgacccg tatttttcaa tcgatagccc aactcaatgt cttcaataga
     2101 agggcgacga taattttcat caaacccgcc tatttccagg aaaatatcac ggcgaatggc
     2161 cccgcaagca ccccaaaacg ttgaagcctc tttttgggcc ttttgatgga cataatggtg
     2221 aaaaagattt ttgtattggg acaggaaatt tatttcggca ggtgtgtcat cgtaggatcc
     2281 gatcaaggca gtgaggtctg gattattgtg aaaggtttct gccacccttc ccagggtatc
     2341 cggatgcaca cacacatcgg catcaacaaa gaagagatat tcgcctttgg cctgaagtgc
     2401 cccaatgttt cgggggtatg ccggtcctcc tgaagtaata ggaagtcgaa tgacctgagc
     2461 accaaactgt ttcgccactt gccaggaccc atcggagtca ccatcggcaa cgacaattat
     2521 ttccatggga ggcggctgta aagcggccag gcttatcagg cattgacgaa aggcctcacc
     2581 cccgttgtac acaggaacaa caattgaaat cgttatttcc gtcaaggaag atctcaacgt
     2641 accactgact ttctgctctt cgtcatttcg atcaatcttg ttcatacctt aagcctaatt
     2701 ggcctgacaa acgaaaccgt ctccgggaaa gcacctcaca gacacttaag tgcaaaaatg
     2761 tgggcaaata ccctgaaaat tcagaaatgg cagggtccga gcccaaatga aaggggcatt
     2821 catggtcaat tcttttccct gcgcaaattg atgtcagagg ctgagggaac ccataactca
     2881 tctagctaac gaccagtcct caatggggtt gcttttcatt cccacaaaat ctttatcggt
     2941 taccagaatg taaacccaag agaaataagc aactatgccc ttaaaatggg tacttatagg
     3001 acaagaaaat tatggtcctc cggctcatgg gcaacacatt ttttggtttg aaaccaggat
     3061 aacctgtagg agaacataat ggaactatga aagttctttg atggggaatc gaggtggttg
     3121 cgggggaggg atttgaaccc tcgacctttg ggttatgagc ccaacgagct accaggctgc
     3181 tccaccccgc gagtcgatag tagaggaggg gcggctgaga agtcaatgca aaaacaggag
     3241 tttagcagaa gaagacacta tggtatcttc accgtcctat gacatttgcc ggaatcattg
     3301 ccatcttgct gtttcttggt cttatcattg gcgattggta tcaatttacc accttgaaaa
     3361 gttgggcctg ccaatatggc tgtccattgg ctcacaggaa gaacgccctg gactctcaat
     3421 ctgtttcctc catttccact gtctttcaaa ctggcgccct tgccctgcct cacggaattg
     3481 cccgctgggt cgcagaccga caggtcatcg tgatccgccc atactatcaa ctcttttccc
     3541 tgcggtttcg caccgcatgg ccattaaagg ggaccatcga tgtaagttca gatggcagcc
     3601 aactcctgct gcatttgtcc aagcgtattc cctggtcatc agcaattctc accctcttat
     3661 ggttactctt tgtcattgga ggcactctca cgtttgtcgt tctgtttatt cttgatggag
     3721 gaataagcac cgtcggggga gtattttggg tggtcgggat gaccgcattg ggcttattag
     3781 tgctggcagg aggcttgatt ctgttgtcgt ttgcctaccg gcttgaggat tcccgcctga
     3841 ctcaggttta tctggaatta ctggacgtct tgactcctcc cttgaaagat ttgaataagt
     3901 cggggagccc ttaaaaaact tcacaaacac aatggcaact ctcttgactc tacctgcagc
     3961 cctgattgct tcagcttagg gaattgtttc aagaagatct ttcttggctt ttgaaaagtt
     4021 ctcaacccgg aacgttggca attcgtacgc ccagcctccc acacgctgat tgaacgcctc
     4081 aacttctcgt tgaacctctt ccggtttttt taatatcgag tcttctttgg aggaaagctc
     4141 atcttgtatt tctgccgttt ttttcgcttc tggagattca ggggcctttt tgtcttgaac
     4201 agaatcctgc gctggaactg tttccccatt ttctgtggaa ggggtagtat ccacaggttg
     4261 aagcagtttt tgatcatatt ccgcagaaaa ttttccaaaa actttatctt cctgtttagc
     4321 cgtttgaaca tgaagtctga gcccgtcaaa ggtttccagt acagccgtca ctccagagcg
     4381 gtcctgaaaa tttatctcat gtccttcttt cacatcctcc agtgagagct gcacgagagt
     4441 ccccactaca ttatttacat taaactggga agccactttg gaacctgcag ggatggaatc
     4501 caactgaaaa tcaagatcct cgggtttatc tttagagaca agaagagttt ccccgcccgg
     4561 atgcctgacc gtcacccgat gcacgcgctt agtcgtcagc gccgttattt ccttatcgag
     4621 ccactctccc gccacttttt ccaacggcaa attccctatg gttaaccagg tttggggatc
     4681 tcctggcttt cgcgcataaa tttcgctcat ccgtggattt cccttggcag gacgttgatt
     4741 ccccagaatc accaaagccg cctcaggatt attcggagtt ttcagcctca ccgttgtcga
     4801 aagtgaacct tcttgttctt tgtcctgtaa ccccagccga tcgtataatt ccggattttt
     4861 cgttttgggt tccgatattc gcaactccgc caacccgatg agagtctgtt ttaccttttc
     4921 cacatcagcg cgatacccgg acttctcctt aacccgccag atattctcct ca
//
LOCUS       DATIAL010000239         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_796496, whole genome shotgun sequence.
ACCESSION   DATIAL010000239 DATIAL010000000
VERSION     DATIAL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796496"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..685)
                     /locus_tag="VLA60_07415"
     CDS             complement(377..685)
                     /locus_tag="VLA60_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09225.1"
                     /translation="MQGEFQFPTDFWRITLPRKFDRVAQKEFEARIQQILSGGYTKLA
                     LDFNLVALISNSGLGQIAMTCLDLKAKGIQVSFVGISPKIKGMLDRHGLSDLLAVAKI
                     "
     gene            1107..1202
                     /locus_tag="VLA60_07420"
                     /pseudo
     CDS             1107..1202
                     /locus_tag="VLA60_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(1305..1898)
                     /gene="idi"
                     /locus_tag="VLA60_07425"
     CDS             complement(1305..1898)
                     /gene="idi"
                     /locus_tag="VLA60_07425"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HSF09226.1"
                     /translation="MNVKEAGPRQMTFSQSDMLILVDKNDEVIGFQEKEACHDGDGLL
                     HRAFSVFLFDQTGRLLLQKRSPHKRLWGDFWSNSCCSHPRRHENTRDAAEKRVREELG
                     VDSCLHFLFKFEYRARYDGRGSEHELCYVYAGLLAGEPKINEFEVGAWRMICAESLDR
                     ELVEKPGAFTPWLKSEWPRLRGSYWSDVKGLLSLENG"
     gene            complement(2085..>2823)
                     /locus_tag="VLA60_07430"
     CDS             complement(2085..>2823)
                     /locus_tag="VLA60_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSF09227.1"
                     /translation="TDTAVGFNVSALLTPLRNSAGEPLVNFGLVYRHQATLHLKGDFL
                     VNGKKVDDAETTLPLPWVLSAGLAVWPIRDAARAWKVEVDVDYVGWSQFKSLDIRLSN
                     APNISQPANWENTYTFSAGTEYKWLKLTSLPHWEIALRGGYQRSNAAVPARTFTPAIP
                     DSNWNIFATGLGLKCKRGSHFLGVISCGDPNPDSGYLEAIVLDLAFQWALWETRTIDG
                     NEISPTINGKYKTKDWYIGSFSIGLIF"
BASE COUNT          733 a          697 c          661 g          732 t
ORIGIN      
        1 agagcgttcg gctgttaacc gaatggtcgc aggttcgagt cctgcccgag gagccaaatt
       61 caatgggctc catgttagac cgccagttgt gcatcgcgtc tgaccgggtg tagacgtttt
      121 gcttggcacc tccctccggt aatctaaccc ttctctgctg accaatccta gctcggaatc
      181 ctttcaggtg tgtagggggc agaacgtcat ccccatcctg gcatttgccg actatacacg
      241 cttggctacc aatccattgt atgggtctgt gaatcagtga atattccatt ggtctacatg
      301 agtcggtttt cagacttgac caaaagggat caaacagaat ggtattgttg atacctccga
      361 agagggaaat aatctcctag atttttgcaa cggcaagaag atctgagagt ccatgtctgt
      421 ccagcatacc tttgattttt ggtgaaatcc caacgaagct cacctgaata ccctttgctt
      481 tgagatctaa acaggtcata gcaatttggc ctaatccaga attgctaatc aacgccacaa
      541 gattgaaatc cagcgccaac ttagtgtagc cacccgaaag gatctgttgg attctagcct
      601 cgaattcttt ttgagctacc ctatcaaact tccgtggaag agtgatgcgc caaaagtccg
      661 ttgggaattg aaattcgcct tgcataacca ttcttctcta acaagacata tggggtggca
      721 atctcgtggc tttcaggaag accggtaaag aatcgaatcc tgtacctatt tagtatgaac
      781 aaaggggatg gcacagaaga ttgagatttt ctctgcaggg ccatttccct aaatcatttt
      841 aatatcttcg cttaataagt acctcccgtc gagcttgttg tggtgagaac ctcagcttca
      901 ccttaaattt cagtgggatc cgtagtgcaa aacaaaggca atgtgccaca gtcacatgcc
      961 aagtcatttt tattctcaaa ggggagacgg atgtacatca aaaggattct caatgctaaa
     1021 cgccgatttc gcagaatata tataggaatt gctcgtactg tattgtcttg accgacgacc
     1081 tgcgcagcga caatgtgttg actggctcaa tattcaagca ctgtcgcatt acccacagca
     1141 aagttttggc agaaacacac aatcaagtta atgacatcga aaactgttgg acccaagcca
     1201 aacaccgtct tcaaatttca tcaaaataat ggccaaaaac tgagaaaagg aataagccca
     1261 atgataatgg gaaccttaaa agcttccttg atcgttgcta ttggttagcc attttctagg
     1321 gacaagagac cttttacgtc gctccagtaa ctacctcgca gacgcggcca ttccgacttg
     1381 agccatggtg taaaggcacc tggcttttca acgagctctc ggtcgagcga ttcggcgcag
     1441 atcatccgcc aggcccccac ctcgaactca tttatcttcg gctctccggc taagagaccg
     1501 gcgtacacat agcaaagctc atgctctgaa ccacgtccat cgtagcgggc acgatattcg
     1561 aacttgaaga gaaagtgcag gcaggaatcg acacctaact cttcacgaac gcgtttttcg
     1621 gcggcgtcgc gggtgttctc atgccggcgc ggatggctac agcagctgtt tgaccagaaa
     1681 tccccccaaa ggcgcttgtg ggggctgcgt ttttgcaaaa gaagccttcc agtttggtca
     1741 aaaaggaaca cggagaaggc acggtgcaat aggccgtcgc catcatgaca ggcctctttc
     1801 tcctgaaagc cgatgacttc atcgttttta tcgacgagta ttaacatgtc tgattgggaa
     1861 aatgtcattt gtcttggtcc agcttccttg acgttcatgt ggtatccgtc atttcccatt
     1921 ttcaaatgag tttggtttcg attctcctca tgagcttaga caaacgcaag gcattccccc
     1981 aacgatacag atagattcaa accgattgct ctatgaaaga atgtagcctt agtcaacgcg
     2041 aaccaactcc atatactggt ggtgtacaga tgtgtatatg tcccttaaaa aatcagaccg
     2101 atactgaatg atccgatata ccagtctttc gttttgtact taccattgat ggtagggcta
     2161 atttcgttcc catcgattgt gcgggtctcc cagagagccc attgaaacgc aagatccaaa
     2221 acaatggctt ccaggtagcc tgagtcaggg ttaggatccc cgcaactgat tacccccagg
     2281 aagtgtgatc cccgtttaca cttcaatccg agcccggtcg caaagatgtt ccaattggaa
     2341 tcaggaatgg caggggtgaa cgtgcgagcc gggactgctg cattagagcg ttgatagcct
     2401 ccccggaggg caatctccca atgggggagt gaggtgagct tgagccattt gtattcggtg
     2461 ccggcactga atgtgtacgt gttttcccaa ttcgcaggtt gcgaaatatt tggcgcgttg
     2521 gaaagacgga tatctaaact tttaaattgg ctccatccga cataatccac gtcgacttct
     2581 actttccagg ctcgagccgc gtctcgaatc ggccatacgg cgagcccggc gctcaaaacc
     2641 catgggagtg ggagcgtcgt ctcggcatcg tccacctttt taccgttcac gagaaagtct
     2701 cccttcagat gaagagttgc ctggtgtctg taaaccaatc cgaaattcac gagaggttcc
     2761 cctgcgctat ttcgcaaggg ggttaaaagt gcactcacat tgaatccgac ggccgtgtcg
     2821 gtt
//
LOCUS       DATIAL010000240         6453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_151903, whole genome shotgun sequence.
ACCESSION   DATIAL010000240 DATIAL010000000
VERSION     DATIAL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151903"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VLA60_07435"
     CDS             <1..289
                     /locus_tag="VLA60_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSF09228.1"
                     /translation="FADDVPEQVKGYRTNQIVELQKEISLSKNKELIGTTVEVMLEGH
                     SKKSDLQWMGHSDHNVTVVWPKDDEIRQPGDLVPVLVMDASPSTLYGHVQS"
     gene            687..1646
                     /locus_tag="VLA60_07440"
     CDS             687..1646
                     /locus_tag="VLA60_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF09229.1"
                     /translation="MDPQILQRIKNCKTLPAIPALPLQVLQLCRDERTTAQRIGDVIS
                     KDPSFVAKLLNVANSSFYGGSRHKVTTVTHAVTLLGMNSIATLAFCFSLYRDLRKKGG
                     GVFDHTHFWHRCILASLAAKILAKRVRVTNEEEVFLAGLLQDLGVLVMSEALGMEYGL
                     LYKKAAQDHQTLQALEQDRWKTDHCEIGAWLAETWELPELLRESVKGSHDPSLASGSD
                     DVSRLTIQCVALSGRLADMWCHQQPEVAIPSAIQLSKELLEIEPDGLVEVAKQIADGI
                     PDMSSFFQISLGKPEDIQKVLDHLIQIVTGISQPEISEPIPAI"
     gene            complement(2025..2537)
                     /locus_tag="VLA60_07445"
     CDS             complement(2025..2537)
                     /locus_tag="VLA60_07445"
                     /inference="COORDINATES: protein motif:HMM:NF019440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HSF09230.1"
                     /translation="MASAYHLWLTPTGQAYDILLKTITDLSTAYHAPVFDPHVTLLGS
                     LPGTEEAISLQCVKLGGLLAPFDIFLAEPAYGDQYFQCVFLKAQETPALMDAHELANR
                     LFVKAPTPFMPHLSLLYGHYSLELKHKITSTLSQALPRNFTVDMIHLIHSKSEHPMDW
                     ASILSVPLSG"
     gene            complement(2604..3350)
                     /locus_tag="VLA60_07450"
     CDS             complement(2604..3350)
                     /locus_tag="VLA60_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09231.1"
                     /translation="MSERKDHQNIVMPEGISFLPPIQVGYEIYYENLEIQGIQDRQAN
                     VWQFIQGSKHLLDWEQEPSNPQDPHAIKIFGVSEQIFSTKRDMLGYVPGDVAKQIVTG
                     GLWPFLQLRLDSISVANQGFVVIRFQILGPIRKKNGDEQPRGGPGIKGERRHTQGTKP
                     TLLFGRESERPKAHRSKVKQVAFFILRIILITLGTALAFVGGTMGTAAWQAKGNLFIL
                     MLVPMALIMIGGLLIWTGIKPSKSKEKTST"
     gene            complement(4213..5637)
                     /locus_tag="VLA60_07455"
     CDS             complement(4213..5637)
                     /locus_tag="VLA60_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSF09232.1"
                     /translation="MKRKNWTFLLMIVGLASALLLTLALESLHAATVPDIRGTWKSGP
                     SSVTVTGCQDPDNNGVYFDPGGDTYSITNQTGANFSFTIVVSESQGGNTIMQTETCSG
                     TVAVDGTVNASCPYVVTVNGGFWYSGIDTLTGSLAGNSLTYTGAGQDLAGDTCQYNYS
                     TATDTRSGPVPPPIVLPINTPYDLNGDGKGDLIWRNTNTGSTAVWLMNGIARASVGFP
                     GGVPFNWQIVGIGDVTGDGKADVIWRNSTNGTVAIWVMNGTTVTSTGFPGSAPTAWAI
                     QAVGDVNGDGKADLIWRNSSDGNTAIWLMNGTARASVAFPGGVPLTWQITGIGDVTGD
                     GKADVIWRNGSNGAVAVWVMNGTTVTSTGFPGSTSTDWKIEGVGDFNGNGKADLIWKN
                     DTSDIVAIWLMNGASIASSKVLGGIASAWKIEQVGDMNGDGKADVVLKNTSTGEVKVW
                     LMNGVALIGIGSPDTVSLSWDIQP"
     gene            complement(6088..>6453)
                     /locus_tag="VLA60_07460"
     CDS             complement(6088..>6453)
                     /locus_tag="VLA60_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09233.1"
                     /translation="SVAELLNTILEDWGYHVCMARNGREGLDMVVNHTVDGILLDMHM
                     PVMNGRTMLDELRWLGYQMPVLAMSGGLDLPALRQLLREGAQGFFLKPFNLQSLKQSF
                     RQIFEKEGSSSFHNHLRLT"
BASE COUNT         1608 a         1566 c         1599 g         1680 t
ORIGIN      
        1 atttgcggac gatgttcctg agcaggtgaa gggatacagg acaaaccaga tcgtggagtt
       61 gcaaaaagaa atttcccttt caaaaaacaa agaactcatc ggcactacgg tggaggttat
      121 gctggaaggg cattccaaaa aatccgacct tcaatggatg ggccatagcg accacaatgt
      181 gaccgttgtc tggcccaagg atgatgaaat ccgtcagcca ggagaccttg tgcctgttct
      241 tgtcatggat gcctcgcctt cgacccttta tgggcatgtc caatcctgat ggatttcctg
      301 tgttcccgaa ggcaaaaata ttcttaaccc catcctccgt ccataaatct tcgattttcc
      361 tgtcattccc tgtgctcggc tcctcctaaa ttgtctcttt ttcccggttg aggccgtggt
      421 tgcggtggag aaatccctta aaccgcaagc tggtttctac ctcaagtcag gtgctcattg
      481 tccgataaaa cctttgaata cttgtggatt tccggtcgta tggcggaaag aattatcctt
      541 caaattcctg gtgctcattc ggaaaagaaa tccctatcca atcgatctat gaagacaagc
      601 ctatacggtg ataagtctgg atgattggtt ttcgaaggca tgctgttgtg ggaaataatg
      661 atacaaggca tgaggtttaa cgaataatgg atccccagat tctgcagcgt ataaaaaatt
      721 gcaagaccct cccggcgatc cccgcgttgc ctctgcaagt cctccaactg tgtcgggatg
      781 agaggacgac cgcccaacgc attggtgacg tcattagcaa agacccgtcc tttgtcgcca
      841 aactgttgaa tgtggcgaat tccagttttt acgggggcag tcgacataaa gtgacgacgg
      901 tcacccatgc ggtgacctta ttgggcatga attctattgc cacgttagcg ttttgctttt
      961 cgttatatcg ggacttgcga aaaaaaggtg gtggtgtgtt tgatcatacc cacttttggc
     1021 atcgatgtat tttggcgagt ttggccgcga agattttggc caagcgcgtt cgggtgacaa
     1081 acgaagaaga ggtgttcctt gccggattat tgcaggactt gggcgtattg gtgatgagcg
     1141 aggcgttagg catggaatat gggttgcttt ataaaaaagc tgcgcaggat catcagacgc
     1201 ttcaggcgtt ggaacaagat cggtggaaaa cggaccattg cgagatcggg gcatggttgg
     1261 ccgagacgtg ggaattgcct gaattattgc gagaatccgt caaggggagc catgacccct
     1321 ccttggcgtc agggagcgat gacgtttccc ggttaactat tcaatgcgtg gccttgtcgg
     1381 ggcggttagc cgatatgtgg tgccaccaac aacccgaggt ggctattcca tcggctattc
     1441 aattatcaaa ggagcttttg gaaatcgaac ctgatggact tgtggaagtg gccaaacaaa
     1501 ttgctgacgg aatccccgat atgtccagtt tttttcagat cagcttgggt aaacccgaag
     1561 atattcaaaa agtgttggat catcttatac aaatcgtaac gggtatctct caaccagaaa
     1621 tttcagaacc catccctgcc atttaagtca gtggttcctc ccaggttttt cctgagtgtc
     1681 tcccggcatt accccgattt tgcttcagta agccgaatgg atttgcacgt ggcggcttat
     1741 gttagggggg aatgggggga caagtccgtt gatttaccag gaaatgccga ggacctgact
     1801 taggagcctg tccgacttcg gatgagtcgg ctgcaaaagt gactaagcgg tccatatttc
     1861 gccgcgtcct tggcccatag tcccactatg ggccggcaaa atcttcaaaa tctgccctcg
     1921 ctcgaccaca ttttcgctat cgattcccag agtccgacag actcctagga gttcttaagg
     1981 caccaggtga cagtcgctcg cgaatgtgta gagggaaaaa gaatttatcc actcagaggg
     2041 actgacagga ttgaggccca gtccatggga tgttcgctct tggaatggat aagatggatc
     2101 atgtcgactg tgaagtttcg gggcaatgct tgagataagg tggacgtgat tttgtgtttc
     2161 agttcaaggg aataatgacc atacaagaga ctgagatggg gcataaaggg ggtaggtgcc
     2221 ttgacgaaca gtctatttgc cagttcatgc gcatccatga gtgccggtgt ttcctgagcc
     2281 tttaagaaca cgcattgaaa gtactgatca ccataggctg gttcggccag aaaaatatca
     2341 aaaggtgcaa gcaatcctcc gagttttacg cattgaaggg atatagcctc ctctgttcct
     2401 ggaagagaac ccaggagcgt cacatgcggg tcgaagactg gagcgtgata cgctgtgctc
     2461 aagtcggtaa tggtcttaag gagaatgtcg taggcctgac cagtaggtgt cagccagagg
     2521 tgataggcgc ttgccatgcc cacctttcag atttgtcgag aagtgcttga atgacgcatt
     2581 cttacgtccc aaaggagtta tttttaggta gaggtctttt cttttgattt tgagggttta
     2641 attccggtcc agatgagcag gcctccaatc atgatcaatg ccattggaac gagcatcaat
     2701 ataaagagat tccccttggc ttgccaggct gcggtgccca ttgtcccgcc cacaaaagcc
     2761 aacgcggtgc caagtgtgat caggataatc cttaaaatga agaatgcgac ctgcttgacc
     2821 ttgcttcggt gcgcttttgg acgttccgat tctctaccga acaagagtgt tggtttcgtg
     2881 ccctgggtgt gccttcgttc ccccttgatg cctggtccac cacgtggttg ctcgtcaccg
     2941 ttttttttcc tgatgggccc aaggatttga aaccggatga caacgaatcc ttgatttgcg
     3001 acagagatgg agtccagccg gagttgtaaa aatggccaga gcccgccagt cacaatctgt
     3061 ttggctacat cacctggaac atagcccaac atatcccgtt ttgtagaaaa gatttgctcc
     3121 gataccccaa atatcttgat ggcatgaggg tcttggggat tgctcggttc ttgttcccag
     3181 tccaacaggt gtttggagcc ttggataaat tgccagacat tggcttgccg atcttgaatt
     3241 ccctgaattt caagattttc atagtagatt tcatacccga cttgaattgg gggcaagaaa
     3301 gaaatgcctt caggcatgac aatattctgg tggtcttttc gttccgacat atttcagctg
     3361 tacctattgc gttgatttaa tcggcagaat tggggagttc tccctcgaaa aaatgtcagt
     3421 tgtcctattt ttttcaacag gggcctctct ttgggcgtgg cgcataaggc tggacttaac
     3481 tccaatcaaa caaggtttct tacaccatac aaaaattgga ggggaaagga aatgtgtaca
     3541 aatatttgta gctaattgac atattgtctt ggtgccgtgt ttttgtttca cccaccagaa
     3601 gagtggcgaa tccctcagaa aaaattggtt gctaggggat ttgatcaaac ggaggtctca
     3661 tttctatcct ggttcataag ggcaggggca gagtgccatg ttgaatctgg aagaagagtt
     3721 atgaggcagg gggactcatg agccaaagag aaaagaattc gttgggaaag tccggatttt
     3781 ttttactgtc gttcttggga ttgggaaaga gtgcctatag gaatgagaat catcgcgtga
     3841 aggtgaaggt aggattattt cataacccat aaaagaatcg cggcaaagag aaagagccac
     3901 caccggacac tcaacgagag tttttctccc tcgctttggt aataaaaacg tttgtccatc
     3961 tcaaatctcc tcagttgatg attttcaaaa tacaggcgta tgttcacgct gaccattaca
     4021 cttaggacta cattccaggc aggggctatt gtgggatggg cctttccaca atgaacaatt
     4081 aggttagaaa aaaaagagcc tgcccaaatg tgggccaggc tcttgggaga cgaaagaaag
     4141 taagcgtggt cttcttcgtg ttcgagcctt gttctccttt ctcagtgatt cagcgagtgc
     4201 attgtcatcc ccctagggtt gaatgtccca ggagagggag actgtatcag gagagccgat
     4261 tccaatcagg gctaccccat tcatcagcca gactttcacc tcccctgtag acgtattctt
     4321 caagaccacg tcggcttttc catctccatt catatccccg acctgctcaa tcttccacgc
     4381 cgaggcgatg ccgcctaaca ctttagacga cgcaatagag gccccattca tcagccagat
     4441 ggccacgatg tcgcttgtgt cattcttcca gataaggtcg gccttcccgt tgccattgaa
     4501 atcgccaaca ccctcaattt tccaatccgt ggaggtgctg ccgggaaatc ccgtgctggt
     4561 cacagtggtt ccattcatca cccacactgc cacggctcca ttgctgccat tgcgccagat
     4621 gacatcggcc ttgccatccc ccgtcacatc gccgattccc gtgatctgcc aggtgagcgg
     4681 caccccgccc gggaaggcaa ccgaggcccg ggcagtcccg ttcatcagcc agatggccgt
     4741 gttgccgtca ctgctgttgc gccatataag atcggctttg ccatcgccgt tgacatcccc
     4801 gacggcctga atggcccagg cagtcggcgc actgccggga aaacctgtcg acgtcacggt
     4861 cgtgccgttc atcacccaga tcgccaccgt gccgtttgtg ctattgcgcc agatgacatc
     4921 ggccttgcca tcccctgtca catcgccgat ccccacgatc tgccagttga acggtacccc
     4981 gcccgggaag ccgaccgagg cccgagcgat cccgttcatc aaccagacgg cggtgcttcc
     5041 ggtgtttgtg ttgcgccaga tcaggtcgcc cttcccatct ccattgaggt cataaggcgt
     5101 atttatgggt aaaacaatag gaggaggaac gggcccactc ctggtatctg tggctgtcga
     5161 ataattatac tgacaggtat ctcctgccag atcctgaccg gcacctgtgt aggtcaagct
     5221 attccccgca agggaacccg ttaaagtatc gattccgcta taccaaaacc ccccgttaac
     5281 tgtaactaca taggggcatg aagcattcac tgtgccatcc acggctacgg tgccggagca
     5341 cgtctccgtt tgcataatgg tatttccgcc ctgactctcc gataccacta ttgtgaaact
     5401 aaagttggcg ccagtttggt tggtaatact gtaagtatcc cctcccggat caaaatagac
     5461 cccattatta tcaggatctt ggcaccccgt taccgttaca ctggagggac cacttttcca
     5521 agtccctcga atatccggca cggttgccgc atgcagactt tccaaggcca acgtcaatag
     5581 aagggcactt gctaaaccca ctatcatcag taaaaaggtc caatttttgc gcttcatgtg
     5641 acgagctctc cttattgaat ccttagcaag ccgcttgcaa caggtgaata tgtaatgagc
     5701 aaaaaatagg ccttttatag ggcaacgaat ctcattagca taaatcgctt ctaaaacggc
     5761 ctgtctacaa aatttgaaaa agaaccggcc aactcagacc cggaagcaat tgtagaaaaa
     5821 aaccacaaac catgatcccg accaatctat tcactggcct tattttcatc aaaaggcaat
     5881 aaatactctt gctgtaaaag atgcgttcca gaatttttgt tagaccaatt ttgccgcgta
     5941 gagcttcgtc ctggaggaat caggcctgac acgaagttgg tgatgccgct tataaaggag
     6001 aaaggtattg caatacatct tgaggggcta taagttgttt gaccgcatag gtcttgatgc
     6061 cacatgttta attcctcttt ctagttttta tgtaagacga aggtggttgt ggaaagaaga
     6121 cgatccttcc ttttcaaaga tctgtcgaaa ggattgtttg agggattgca ggttaaacgg
     6181 cttaagaaag aagccttgag cgccttccct tagaagctgc cgaagggctg ggaggtctag
     6241 cccaccggac attgccagta cgggcatttg atatcccaac catcgtaatt catcgagcat
     6301 ggtgcgcccg ttcattacgg gcatatgcat atccagcaaa atgccgtcaa ccgtatgatt
     6361 cacgaccata tccaaacctt cccgtccatt tctcgccata cagacgtgat atccccaatc
     6421 ttcaaggatc gtatttagta actctgccac tga
//
LOCUS       DATIAL010000241         4049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1550134, whole genome shotgun sequence.
ACCESSION   DATIAL010000241 DATIAL010000000
VERSION     DATIAL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550134"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VLA60_07465"
     CDS             <1..291
                     /locus_tag="VLA60_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09234.1"
                     /translation="KNFHANDLPLKSKLLWFVIEWKIAFILEGRVPRGCYESVKLRSR
                     RGKNERDQCSGLLSRINFNEPVWGGTDSAEKIKKTEEVRKQRSGRRKGERKI"
     gene            complement(288..1670)
                     /locus_tag="VLA60_07470"
     CDS             complement(288..1670)
                     /locus_tag="VLA60_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSF09235.1"
                     /translation="MKHLFMALVCCGLLAQTQAWAVDPLYEDSLENVLLEKGIITKED
                     WVRIQAEREKREQELNTRMDQEFPVTVGYGRRGFEFATKDGKFATQIQWRFQGRWSYP
                     TDSDPISAADFNDDPQSTFQLRRVRMKVGGHGYQPWIRYYFEMNWQSTSESGSSGVSP
                     QLLDWRISLEKFSFLSLQLGQWKVDYNRERRDSSGNQQFVERSIVNEIFNIDRQVGAM
                     LYGHVAPGTAFDSRYYAGVFTGSGRGEANDDANMMYFGRYQWNFLGRDLKWSQSDVEF
                     HEQPTGSVAFGAYTTIGKCTRWSTGGCGTLPENNSAGVPYTSDSSAKAGQYRVQGMME
                     EFAFKWRGLSIQHEYHWKEVKDNSYPEGAPGYKTNMMGSYSQVGYFPHYLIPAVPKPL
                     EVAFRYAFVDPNISAPNDRRQEYTTAINWFFAGHANKITLDGSWLTLAQPAGQNQHEQ
                     RVRLQWDVSF"
     gene            complement(1756..2625)
                     /locus_tag="VLA60_07475"
     CDS             complement(1756..2625)
                     /locus_tag="VLA60_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HSF09236.1"
                     /translation="MDIIQSTPRIIPDPGEQSGLRAWLPYPSTEPCCDPVPHIRAKNL
                     SIAYQGHQVLQNVTLEIHRGCITALVGPSGCGKTSFLTSLNRLTDLIPGCQVSGNLHM
                     QELDVLAPTTDVLQLRRTVGMIFQKPTVFPFSIRKNLELPLREHGTYDRTLLASAVEK
                     VLQQVGLWDEVKDRLDHPAQALSGGQQQRLCLARALVLAPDVILLDEPCSALDPLSSG
                     IVEDLIVALRGHVTVIIVTHNLAQARRIADHIAFFWSCDGVGQLIEYDTAQAIFETPA
                     HELTAAYISGARG"
     gene            complement(2595..3449)
                     /gene="pstA"
                     /locus_tag="VLA60_07480"
     CDS             complement(2595..3449)
                     /gene="pstA"
                     /locus_tag="VLA60_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HSF09237.1"
                     /translation="MSRQSRYLQEYGTTAFIWMAATLVAVPFFCLIGDLLWNGLNHLS
                     FRFVFSAPQNAGREGGIGPILISTLLILGVCMGVALPMGLGTALFLSEYTRHDHVLGR
                     LIRGSLDVLAGVPSIVFGLFGNALFCQILGLGFSILSGGLTLACMVLPILIRTVEESF
                     RAISNEQRLSAAALGLSKPTTIRTILLPAAMPGLLVGVILGLGRAIAETAALIFTSGY
                     VDRMPESLLDSGRSLAVHIYDLAMNVAGGNPHAYATALILVSLLFIINFTSSRIATRW
                     ISFNQLPA"
     gene            complement(3433..>4049)
                     /locus_tag="VLA60_07485"
     CDS             complement(3433..>4049)
                     /locus_tag="VLA60_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSF09238.1"
                     /translation="LGILSALFCHFYAPPLLSRWYRRMVELLAGIPSVVFGFWGLVVL
                     VPIIGRWHPPGTSLLAGILILTLMIIPTIMLVAHASIAHIPIAYIHSASALGFSRGSL
                     IWHVVLPTTKVGLFTGILLGLARALGETMAVLMVCGNVVQTPSHLFDPIRTLTANIAL
                     EMSYALADHRAALFVSGLFLMGLVVLVVSLAEGLRHRNIYVPAK"
BASE COUNT         1049 a          989 c         1124 g          887 t
ORIGIN      
        1 aaaaactttc atgcaaacga cctcccctta aaaagtaagc tcctatggtt tgtaatcgag
       61 tggaaaattg cattcatcct ggaggggaga gtaccccgag gttgttacga aagcgttaag
      121 ttgaggtcaa ggagaggtaa aaatgagcgt gatcagtgtt ccggtctatt gagtaggatc
      181 aacttcaatg agccggtttg gggaggaacg gattcagcag aaaaaatcaa gaagacagag
      241 gaagtgagaa agcaaagaag tggaaggagg aaaggtgaac gaaaaattta gaacgagacg
      301 tcccattgca agcggacccg ctgttcatgt tggttttgac ctgccggttg ggccagcgta
      361 agccaggaac catcaagtgt gatcttgttt gcgtgcccgg caaaaaacca attgatggcg
      421 gtggtgtatt cctgccgccg gtcattggga gcggaaatgt tggggtcgac gaatgcatag
      481 cggaaggcca cttccaatgg ttttggcacc gccggaatga gatagtgggg gaaatatccg
      541 acctgcgaat aactccccat catgttggtc ttatagcccg gcgccccttc cggatagctg
      601 ttatccttaa cctctttcca atggtattca tgttgaatag acagcccccg ccatttgaag
      661 gcgaattcct ccatcatgcc ttgcacccgg tattgtccgg ctttagcgga agaatcagag
      721 gtgtagggca caccggcgga attgttttcg ggcaacgtgc cgcatccgcc tgttgaccat
      781 cgggtacact tgccgattgt ggtataggct ccgaaggcca cgcttcctgt aggttgctca
      841 tgaaactcca catcactttg tgaccatttc agatcccggc ccaaaaagtt ccattgatag
      901 cgtccgaaat acatcatatt agcgtcgtcg ttggcttctc cccggccgga tccggtaaag
      961 accccggcat aataccggga gtcaaaggcc gtcccagggg ccacgtggcc atacagcatg
     1021 gctccgacct gtcggtcgat gttaaagatc tcattgacga ttgatcgttc cacgaactgc
     1081 tggttgccgg aggagtctct tcgttcccga ttatagtcga ctttccattg gccgagttgc
     1141 agggaaagga acgaaaattt ttcgagcgag atccgccaat ccagcaattg gggactgaca
     1201 ccggaactgc cggattcgga agtcgactgc caattcattt cgaaataata tctaatccag
     1261 ggttgatagc cgtggccacc gactttcatt cgaacccgtc gaagttgaaa ggtactctgt
     1321 gggtcgtcat taaagtctgc cgcagagatg ggatcggagt ctgtgggata ggaccagcga
     1381 ccctggaagc gccattgaat ctgggtggca aatttgccat ccttcgttgc aaattcaaac
     1441 cctcttcttc cgtatcccac cgtaaccgga aactcttgat ccatacgggt attaagttct
     1501 tgctctcttt tttcccgctc ggcctgaatg cggacccaat cttccttggt aatgattcct
     1561 ttttccagta acacattctc cagactgtcc tcatagagag ggtccacggc ccaggcttgc
     1621 gtttgggcta aaagcccgca acataccaat gccataaaca ggtgtttcat tcctatagtc
     1681 tccaatgggt cgtgacggca ggagatcgaa attgaagtga gaaattacaa tattaagggc
     1741 cggggcacaa gagatttatc ctctggcacc ggaaatatag gctgcagtga gttcatgggc
     1801 aggagtctca aatatggctt gggcggtgtc atattcgatg agttggccaa ccccgtcaca
     1861 tgaccagaaa aacgcgatgt ggtccgcaat gcgtcgggcc tgggccagat tgtgagtgac
     1921 aataatgacg gtaacatgac ctcgcaaggc gacgatcaga tcttcaacga ttccgctgga
     1981 caagggatcc agcgcactgc agggctcatc aagcagaatg acatccggcg ccaacacgag
     2041 tgctcttgcc aggcagagtc tctgttgttg tcctcccgac aaggcttgag cgggatggtc
     2101 gagccggtct ttgacttcat cccagagacc aacttgctgg aggacctttt ccacggcgga
     2161 ggccaagagc gtgcggtcat acgttccgtg ttcacggagg gggagttcaa gatttttgcg
     2221 gatcgaaaaa ggaaagacgg tgggtttttg aaaaatcatg cccacggtac gacgcaattg
     2281 gaggacatcg gtcgtcggag cgaggacatc cagttcctgc atgtgcaggt tccccgaaac
     2341 ctggcatcct ggtatcaaat ccgttaagcg attgaggctc gtgagaaaac tggttttccc
     2401 gcaacctgaa gggcccacta acgctgtaat gcacccccga tgaatttcaa gggtgacatt
     2461 ctgaaggacc tgatgaccct gataggcaat agaaagattt ttcgcgcgaa tatgggggac
     2521 aggatcacag cagggttcgg tggatgggta gggaagccat gctctcaatc ctgactgttc
     2581 cccgggatca gggattatgc ggggagttga ttgaatgata tccatcgtgt ggcaatccgg
     2641 gaagaagtaa aatttatgat gaacaagaga cttaccaaaa tgagagcggt ggcataggca
     2701 tgggggttgc ctccggccac attcatggcc agatcgtaaa tatgcacagc aagagatcgt
     2761 ccggagtcca gcaacgattc cggcatccgg tccacgtatc cactggtaaa aatcaaggct
     2821 gcggtttctg caatggctcg tccgagtccg aggatgactc ccacgagaag gccgggcata
     2881 gccgcaggga ggaggattgt tcgaatcgtg gttggttttg aaaggccaag ggccgctgcg
     2941 gataatcgtt gctcattgga aatggcgcga aaactttctt ccaccgtacg aattaaaata
     3001 ggtaacacca tgcaggccaa tgtcagccct ccagacagaa tggaaaatcc caatccgagg
     3061 atttggcaga acagggcgtt gccaaacagt ccgaagacaa ttgacggcac gcctgccagc
     3121 acatccagtg agccgcggat cagtcggccc aggacatgat cgtgacgggt gtattcggaa
     3181 agaaacagtg cggtccccaa tcccataggt aaggccactc ccatacatac tccgaggatc
     3241 aacaacgtgg atatgaggat ggggccgatt ccgccttccc gccccgcatt ctgtggagca
     3301 gaaaacacga agcggaatga cagatggttc aacccgttcc agagtaaatc acctatcaag
     3361 cagaaaaagg gtacggcgac cagggtggcc gccatccata taaacgcggt cgtgccatat
     3421 tcttgtaggt atctactttg ccgggacata gatattccga tgtcgcaagc cttctgccag
     3481 gctgacaacc aggacgacga gtcccatgag gaagagacca ctgacaaaca atgcggcccg
     3541 gtgatcggcc agcgcatagg acatttccag ggcaatgttt gctgtcaggg ttctgatcgg
     3601 atcaaacaga tgggacgggg tttggaccac gtttccgcag accatcagaa cggccatggt
     3661 ttctcctagc gctcgagcca atcccaacag gataccggtg aagagtccaa cttttgttgt
     3721 gggaagaacc acatgccaaa tcaatgaccc acgactgaat cccaaggctg aggcactatg
     3781 aatataggcg atgggaatat gggcaatact cgcgtgggcc actaacatga tggtgggtat
     3841 gatcatgagc gtcagaatga ggatgccggc cagcaggctt gttcctggag gatgccagcg
     3901 gccgatgatc gggaccagaa ccacgagacc ccagaagccg aacacgactg aaggaattcc
     3961 tgccaggagt tcgaccatgc gtcggtacca tcggctgagt agaggggggg cataaaagtg
     4021 acaaaagagg gctgacagaa tgccaagcg
//
LOCUS       DATIAL010000242         6650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_904764, whole genome shotgun sequence.
ACCESSION   DATIAL010000242 DATIAL010000000
VERSION     DATIAL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904764"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VLA60_07490"
     CDS             <1..815
                     /locus_tag="VLA60_07490"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HSF09239.1"
                     /translation="LRDLNSLIVVIKKIQPDWIFHLAAQSFVTTSFDAPVDTLSTNVL
                     GTTNLLDALRIAGVDAKVHVCSSSEVYGQVLAHEVPITEANPFRPASPYAVSKVGEDM
                     IAFQYYLSYGIRTVRTRMFTHTGPRRGDVFAESAYAKQIVEVEYGLRKNPIRVGNLDS
                     IRTFADVRDAVRAYWVLMEKCPAGEVYNIGGNRTMTLRDMLEILKGMAACRIEHVVDP
                     ALLRPSDVTLQIPDTSKFKAATGWEPRIPFEETLQGILDFYRRRYHKRSKAA"
     gene            812..1933
                     /locus_tag="VLA60_07495"
     CDS             812..1933
                     /locus_tag="VLA60_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HSF09240.1"
                     /translation="MIISRTPYRLSFFGGGTDYPQWYLREEGAVLSTTIDKYCYVSCR
                     YLPPFFNIKHRIVWSHIETVSTIGEILHPAVREGLRFLGFDDSQGVEIQHQGDLPARS
                     GIGSSSSFSVGLVKALTGLKGEMLGKMDLARQAFTLEQDILKETVGSQDQVAAAFGGF
                     NVIRFLRDGQIQVEPLTLSLGRLAELEQNLVLLYTGTSRLGSDIAKSVTKNFSLRSAE
                     LKTMRVMVDRGLGLLSGTSSLEEFGKLLHESWMLKRALSHDISNSTIDEIYKNALGNG
                     AIGGKLSGAGGSGFMLFYVPISKQAHFLSAMHPYICVPFSFSQSGSSIIYYDGPDRRV
                     SGGYPSEGEVVHKEMERRRLPSLKTPKKAKEKVLVGHRS"
     gene            1947..2507
                     /locus_tag="VLA60_07500"
     CDS             1947..2507
                     /locus_tag="VLA60_07500"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSF09241.1"
                     /translation="MPSRTSVPPQMGMTKEVFGCPWFRVHEEAWDDFSVLDRQPFYRI
                     ESSNGVLVLALTKNEEIILVRQFRQALRRNTIEFPAGSIEDGESPEEAAARELFEETR
                     YRAGTLRLLGSGHIMMNRYSARDFLFMAQDCEVLSHTPEVPEQEVLLVSPPELKALVT
                     SGDFEHIPALSLFSLVEWQFGLRLVV"
     gene            2509..3375
                     /locus_tag="VLA60_07505"
     CDS             2509..3375
                     /locus_tag="VLA60_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HSF09242.1"
                     /translation="MNIQALEYKAAELRLHILRTALKAGKGHVPPAFSWVEIGVALFY
                     GGHLNLRSHDPKWENRDRFILSKGHGCLTLYAMLADLGFFPKTELDNFCGPGSLLAGH
                     PDTQIPGVEGVSGSLGHGLGLSAGLALGAKLHSCPWKVVTVMGDGECQEGSVWEAAMF
                     ASHHQLHNLVAIIDRNGLGATNYTEKNVALEPFVSKWKAFGWEVVSINGHSFEELDKV
                     LGCFRQRQSLKPLMILAQTVKGKGLSFMEASVDWHHRIPKGEEVEEAIRQLRAAISSS
                     GSVYTMEMAHGA"
     gene            3365..4309
                     /locus_tag="VLA60_07510"
     CDS             3365..4309
                     /locus_tag="VLA60_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSF09243.1"
                     /translation="MAHDFRDAVFDSVLQIVAKNPRTIVLTNDMGAMGLSQIQELYPR
                     QVLNVGISEQNMMSVAAGMALSGYVVFVYGIASHITTRCYEQLKLDVCALKVPVILLG
                     MGPGLSYGVDGPTHHATHDCALLQTLSGMTIYLPADGVAIRGMVEQAYQSGMPAYIRI
                     DKDPYEPIYTPNEHDFSLGLETIQQGESLCVVTNGIMLARVREAARELREEGIEVALV
                     DLYRMNPCNESRLWEACRNAHAIVTVEEHGRTGGIGSLVGRLLSERGHAIPFCGLSLG
                     DEMLFGSASRTWAYGQYGLEKESLKNTFRQMALLPTTV"
     gene            4345..5352
                     /locus_tag="VLA60_07515"
     CDS             4345..5352
                     /locus_tag="VLA60_07515"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF09244.1"
                     /translation="MKTSSSINFGKRILVSGGTGMIGIPLVRALLELGAQVTVVSLEQ
                     ESYARAVLGPSVAFFRGDLTCYDTCEKAVKGQDYVFNLVGIKGSVGIGVKRAASYFVP
                     MLRYQSNLLEAAYRGGVSRYLFVSSICAYPPATLHVEDNVWNGLPKQNDRYAGIAKRI
                     GELEAETYMNEYNWEAVRVVRPSNVYGPLDDFNPATGQVIPALIRRVLDGESPVRIWG
                     DGSVIRDFIFVQDVVAGLLLAMEHAPPCFPINLGSGVPTTIRQLAETIVAESRVSTEI
                     EWDPSKPAGDPVRLLSMERAEATIGFRPKISLREGIRQTIHWYRENRELAHERKNLLS
                     A"
     gene            5324..5791
                     /locus_tag="VLA60_07520"
     CDS             5324..5791
                     /locus_tag="VLA60_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09245.1"
                     /translation="MSGKTSLAPKNRKRKTEGLSSPRFVPGLGDLVDRMTVDQLKEAF
                     HESIRTSVRTEIADLEYDIDGWADRHQIKLSGRNVRIIIVLAQINYEIWMCKEGMNAE
                     PKRYDQWLKRAHQLNGIRNQMKNLLLAEVKTGTRLCLASNVSVDGLKDWNIQL"
     gene            5807..>6650
                     /locus_tag="VLA60_07525"
     CDS             5807..>6650
                     /locus_tag="VLA60_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSF09246.1"
                     /translation="MLLKKLGQSGIEISAIGQGTGIHHSLTNRGVYWELESTIRSGID
                     MGITFIDTAPVYGDGASEAVIGKALKGIRHGAVLATKVSPENLSATGIKNSVKGSLRR
                     LQTDRIDLLQVHWPNPQIPISDTLLGLMDMVQEGLVRYVGICNFSFKDTQEIHQQLTS
                     KFLASVQVEYNLFDRSIEREFLPYGEQEGISIIAYSPLHRGRIVANRRQYAVLQELAN
                     KYHKTPAQIGLRWLTGHLPVVVIPNTTNVQRMKENAESMDFDLIDEDVRYLEAQCTGH
                     FLEIS"
BASE COUNT         1648 a         1427 c         1837 g         1738 t
ORIGIN      
        1 atcttcggga cttgaattcg ctcattgtcg tgatcaaaaa aatccaaccc gattggattt
       61 ttcatttggc ggcacaatcc tttgttacga caagttttga cgcgccggtc gacacgctgt
      121 ccacgaatgt gctcgggacg accaatttgc tcgacgcgct ccgcattgcc ggagtggatg
      181 ccaaggttca tgtctgcagt tcctctgaag tgtacggaca ggtgttggcg catgaagttc
      241 ctatcacaga ggccaatccc tttcgtccgg caagcccgta tgcggtgtcc aaagtcggag
      301 aagacatgat tgcctttcaa tattatttgt cctatggcat tcgaaccgtc cgtaccagga
      361 tgtttaccca taccggccca cggcgagggg atgtctttgc ggaaagtgcc tatgccaagc
      421 aaattgtgga ggtagagtat gggcttcgga aaaatccgat ccgggtcgga aatttggaca
      481 gtattcggac atttgccgat gttcgcgatg ccgtcagggc ttactgggtg ctgatggaaa
      541 agtgtccagc cggggaagtt tataatatcg gaggtaaccg tacgatgaca ctgcgggaca
      601 tgctcgaaat tctcaaaggc atggccgcct gtcggattga gcatgtggtg gatcccgcat
      661 tgttgcgacc gtcggatgtc accttgcaga ttcccgatac atcaaagttc aaagcggcga
      721 ccggatggga accacgcatc ccctttgaag aaaccttaca aggaattctg gatttttatc
      781 gaaggcgtta ccacaagagg agcaaagctg cgtgattatc tctcggaccc cctatcgatt
      841 gtcgtttttc ggaggaggca cggactatcc tcaatggtat cttcgggaag agggcgctgt
      901 tctttccacc acaattgaca aatattgcta tgtgagttgt cgataccttc ctccattttt
      961 taatattaaa caccggattg tctggtcgca tattgaaacg gtatcgacta ttggagagat
     1021 tctgcatcct gccgttcgag aaggcctgcg atttttaggt tttgatgatt ctcagggagt
     1081 ggaaattcag catcagggtg atctgccggc ccggtcaggc attggatcca gctcttcatt
     1141 ttcggttggt ctggtgaaag ctctcactgg gctgaagggt gaaatgttgg ggaaaatgga
     1201 tctggcccgc caggcgttca ctttagagca agatatctta aaagaaacag ttggctccca
     1261 ggatcaagtc gcggccgcct tcggcggatt taatgttatt cgttttctgc gagacggcca
     1321 gattcaagtc gaaccgctca ccttatcttt ggggcgattg gctgaactgg agcaaaatct
     1381 cgttctactg tataccggca ccagtcgcct gggttcagac attgcaaaaa gtgtcaccaa
     1441 gaacttttct ctcaggtctg ccgaactgaa aaccatgcgt gtaatggtgg acagggggtt
     1501 gggtctttta agtggaacga gttcgctgga ggagttcggg aaattgcttc atgaaagctg
     1561 gatgctgaaa cgggcacttt cccacgacat ttccaattcc actattgacg aaatttataa
     1621 aaatgccctg ggcaatgggg cgattggtgg aaagctttcc ggggcaggag gcagtgggtt
     1681 tatgttgttt tatgttccga tttccaagca ggcacatttt ctttcagcga tgcatcccta
     1741 tatctgtgtg ccgttttctt tctcacaatc aggaagcagc ataatttact acgacggacc
     1801 tgatagaagg gtatccggcg gatatcctag cgagggagaa gtggtccata aggaaatgga
     1861 acgaagacgg cttccctctc tcaagacccc taaaaaggca aaggaaaaag ttcttgttgg
     1921 gcaccgatcc tgagaagcat gaccacatgc cgtcacgaac ttccgttccg ccacaaatgg
     1981 gtatgaccaa ggaggtattc gggtgtccgt ggtttcgggt acatgaagag gcatgggatg
     2041 acttttctgt cttggaccgt caaccgttct atcgtattga aagctcaaat ggtgtcttag
     2101 tgttagcctt gaccaaaaat gaagagatta ttctggtgcg gcagtttcga caggcgttgc
     2161 gcaggaacac cattgaattt cctgctggga gtattgagga cggtgaatct cccgaagagg
     2221 ccgcggcgcg ggaactattt gaagaaacgc gatatcgagc tggaactctt cgtttgttag
     2281 ggagcggaca tatcatgatg aatcggtaca gtgccagaga ttttttattc atggctcaag
     2341 attgcgaggt gctttcccac actcccgaag ttccggaaca agaggtgcta ctggtttcac
     2401 ccccggagct taaagccctc gtgacatccg gagattttga gcatattcct gcgttatccc
     2461 ttttcagcct tgttgagtgg caattcggtt tgcgtttggt ggtatgacat gaacattcag
     2521 gcccttgagt acaaagccgc agagttgaga cttcatattc tcagaaccgc gttaaaagcc
     2581 gggaaaggac atgttccccc ggcattttcg tgggtggaaa ttggtgtggc gctgttttat
     2641 gggggacacc tgaaccttcg gtcccatgat ccaaaatggg aaaaccggga tcgattcatt
     2701 ctgagtaaag ggcatggatg tttgaccttg tatgcgatgt tggccgattt ggggttcttc
     2761 cccaaaaccg agttagacaa tttttgcggt ccgggaagtc tcctggccgg tcaccctgat
     2821 acacaaattc cgggagtgga gggggtttcg gggtcactcg ggcatgggtt aggcctgagt
     2881 gctggattgg ccttgggagc taaacttcat tcatgccctt ggaaggttgt gacggtcatg
     2941 ggggatgggg aatgtcagga gggatccgta tgggaagcgg ccatgtttgc gagccatcac
     3001 caactccata acctggtagc gatcattgat cggaatgggt taggtgcgac caactatacc
     3061 gaaaaaaatg tggcccttga gcccttcgtc agcaaatgga aggcttttgg atgggaggtg
     3121 gtgtccatca atgggcattc ctttgaagaa ttagacaagg tcttggggtg ctttcgtcaa
     3181 aggcaatcgc tgaaacctct gatgattttg gctcaaacag tgaagggaaa agggttgtca
     3241 tttatggaag cctcggtcga ttggcatcac cggattccga agggtgagga agtggaagag
     3301 gccattcggc agctaagggc tgcaatttct tcatcgggat cggtgtatac catggagatg
     3361 gctcatggcg catgattttc gtgatgcggt ctttgacagt gttttacaga ttgtggccaa
     3421 aaatccccgg acaattgtcc tcaccaatga tatgggagcc atgggattaa gccaaattca
     3481 ggaattgtat cctcggcaag ttttgaatgt gggaattagc gagcaaaata tgatgagtgt
     3541 ggcggccgga atggcacttt ccggatatgt ggtttttgtc tatggaattg cctcccatat
     3601 tacgacgcgg tgttatgaac agttgaaatt ggatgtgtgt gccttaaagg ttccggtcat
     3661 tcttcttgga atggggcccg gcttgtcata cggggtggat ggacccaccc atcatgcgac
     3721 acatgactgt gctctcctcc aaacactatc aggcatgacc atttatctgc ctgcagatgg
     3781 cgtagccatc aggggcatgg tagagcaagc ctaccagagt ggaatgcccg cctatatccg
     3841 gatcgataag gacccctatg aacccatata tacccccaat gaacatgact tcagtctagg
     3901 acttgaaacg attcaacagg gggaatccct ctgtgtggtg accaacggca tcatgctggc
     3961 cagggtgcga gaagcggcaa gagaattgcg ggaggaaggt atcgaagtcg ctcttgtcga
     4021 tttatatcga atgaatcctt gcaacgagtc cagactttgg gaagcgtgtc gaaacgctca
     4081 tgccattgtg acggtagaag aacatggtcg gaccggaggg attggaagtt tggttgggag
     4141 actgttgagt gagcggggtc acgctattcc tttctgcggg ctttcgttgg gtgatgaaat
     4201 gttgtttggc tccgcgagtc gaacctgggc ctatgggcaa tatggcctgg aaaaggaaag
     4261 cctgaaaaat acgtttcgac aaatggccct gttacccacg actgtctgat ttttcttcta
     4321 tcgattgaag taaggatatt catcgtgaag acgagttcgt ccatcaattt cggaaaacgg
     4381 attctggttt ctggaggcac ggggatgatt gggattccac tagttcgagc cttgttggaa
     4441 ctgggggctc aggtgaccgt ggtgtccttg gagcaggaaa gttacgcgag agccgtatta
     4501 ggcccttcag ttgccttctt taggggcgat ctgacttgtt atgacacatg cgaaaaagct
     4561 gtgaaagggc aggattatgt ttttaatttg gttggaatca aagggtccgt cggtataggg
     4621 gtgaaaagag cggccagtta ttttgtgccc atgcttcgat accaatccaa tctattagaa
     4681 gccgcatatc gaggaggagt ctcccggtat ctttttgtga gcagtatttg tgcctatcca
     4741 ccagcgacac ttcatgtgga ggacaacgtc tggaatggtc ttcccaaaca aaatgaccgg
     4801 tacgctggaa ttgcgaaaag gatcggcgaa cttgaagccg aaacctacat gaacgaatac
     4861 aattgggagg ccgtccgagt ggtccggccc tcaaatgtgt atggtccgct tgatgacttt
     4921 aatcctgcaa cgggacaggt gattccggct ctcattcgca gggtattgga tggagagtct
     4981 ccggttcgta tttggggaga tggatccgtc attcgcgatt ttatttttgt tcaggatgtt
     5041 gttgccgggt tgttgctggc catggagcat gctccgccct gctttcccat taatttggga
     5101 agtggtgttc cgaccacgat cagacaatta gctgaaacca ttgttgctga aagtcgggtt
     5161 tccactgaaa ttgagtggga tccctcaaag cccgctggcg accctgtccg gttattatcg
     5221 atggaacggg cagaagccac cattggattc cgtcccaaaa tctctctaag agaagggatt
     5281 cgtcaaacga ttcactggta tagagaaaat cgggaacttg cccatgagcg gaaaaacctc
     5341 cttagcgcct aaaaacagga agcggaaaac cgaaggactt tcaagcccac gatttgtgcc
     5401 aggattggga gatctggtcg atcgtatgac cgtggatcaa ctgaaggagg cgttccatga
     5461 atcgatccgg acatctgtca gaaccgaaat cgctgacctg gaatatgata ttgatggatg
     5521 ggctgatcga catcagatca aattgtcagg gcggaacgtc cgcatcatta ttgtgcttgc
     5581 gcaaatcaat tacgaaatct ggatgtgtaa agagggtatg aacgccgaac ccaaacgata
     5641 cgatcagtgg ttgaaaaggg ctcatcagtt aaatgggatc agaaatcaga tgaaaaattt
     5701 acttctagcc gaagtgaaaa cgggaacccg attgtgcctg gcgagtaatg tctctgtgga
     5761 tggtttaaaa gattggaata tacagctgta actttgagtt tgcactatgc tcctcaaaaa
     5821 attgggccaa tctggaatag aaatttcggc gatcggtcaa ggaaccggga ttcatcattc
     5881 tctaacaaat cgaggggtgt attgggaact tgaatccacg attcggtccg gaattgatat
     5941 gggaataacc tttattgata cggcacctgt gtatggagat ggggcatccg aagcggttat
     6001 tggtaaggct ttaaagggga ttcgtcatgg tgcggtgttg gcgaccaagg tctctccgga
     6061 gaacttatct gcaacaggca tcaaaaattc cgttaagggg agtctccggc gtctgcaaac
     6121 agatcggatt gaccttctcc aagtccattg gcccaatccc caaatcccca tctcggatac
     6181 cctgttgggc ctgatggata tggtacagga ggggttggtc cgctatgtgg gaatctgcaa
     6241 tttttcattc aaagacactc aagaaataca ccaacagtta acctcaaagt tcttagcctc
     6301 tgtccaggtc gaatataact tgttcgatcg aagtatcgaa cgggaatttc tgccatatgg
     6361 ggaacaagag ggaatcagca ttattgcgta tagtcctctt catcgtgggc gaattgtggc
     6421 gaaccggagg cagtatgctg ttcttcagga gttagcgaat aaataccata aaactccagc
     6481 tcaaattggt ttacggtggt tgaccgggca tctgcccgtg gtggtgattc caaataccac
     6541 gaatgttcag cgaatgaagg aaaacgcgga aagtatggat tttgatctaa tagacgagga
     6601 cgtccggtat cttgaagcgc aatgtaccgg acattttctg gagatttctc
//
LOCUS       DATIAL010000243        12524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_474992, whole genome shotgun sequence.
ACCESSION   DATIAL010000243 DATIAL010000000
VERSION     DATIAL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12524
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474992"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1279
                     /locus_tag="VLA60_07530"
     CDS             47..1279
                     /locus_tag="VLA60_07530"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF09247.1"
                     /translation="MNTGIANYGNIFVGRQPILGPNLKTIGYEVLYRDCESDSANIHD
                     EAMATAQVLLNTYLDIGLEHVVGTHLAFLNIPTQFLLDRHCEALPKHRVVLEILETVE
                     PTQEVIEAMTSLSQQGYTIALDDFVFHDRFRPFLELADIVKIDVLDKSFEQLQHETHQ
                     FRNYRARLLAEKVETREAFETCKQLGIFYFQGFFFYRPDIIRGHKIPANRVALLELLG
                     KIQDPNSTFESLVECIRNDLSLSYKILRYVNSASVGLPRRVESIEQAACMVGLDRIRT
                     WASLIVMAIGKDQPMEMLVIALVRAKMCESLGQQLGADSPEKYFTMGLLSVLEALYGS
                     SMEKLVGNLPLPDDILEALILEKGTMGTALSSVKAYEKGEWLQLKTLQLSPGTIRDFY
                     VQAIDWANHFSPMIEEPA"
     gene            1496..2410
                     /gene="lipA"
                     /locus_tag="VLA60_07535"
     CDS             1496..2410
                     /gene="lipA"
                     /locus_tag="VLA60_07535"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HSF09248.1"
                     /translation="MTFIPTSEITPVSSSGKTSRRRNLPSWFKVRFRPGPHYQEIRQL
                     MDTHRLHTICEEARCPNIWECWNNRTATFLILGDICTRRCHYCSVTTGRPSAVDREEP
                     QRVADAVKLLKLRHAVVTSVNRDDLPDGGAALFAETIQRIRHENPACTVEVLISDFQG
                     HQTDLEIVMQAKPDILNHNIESVPRLFPSIRPQGKYQRSLQVLDWAKAMGGRTKSGLM
                     AGLGESNDEIRQVMQDLRKVDCDILTIGQYLQPTLQHAPVSRYYSPEEFEEFKQEGTA
                     LGFQHVESGPLVRSSYHAEEQTHGQTCP"
     gene            complement(2467..2634)
                     /locus_tag="VLA60_07540"
     CDS             complement(2467..2634)
                     /locus_tag="VLA60_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09249.1"
                     /translation="MMKCETCCQEYTQFDFVTLRNLCEHVYLDSLAQKQNPSYAKSDK
                     PVSRLDLHQPV"
     gene            complement(2877..3221)
                     /locus_tag="VLA60_07545"
     CDS             complement(2877..3221)
                     /locus_tag="VLA60_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09250.1"
                     /translation="MKPLTKLFILMVVMGCFQGVLSGLAMEGEQMSQTPQVRQELQTN
                     SPTSTARYVNVEGTLQAIQGDLFIIEGASAEKQIKIQVGKDTAFPNGQKELGQPVNAL
                     VSAPDGHALIIR"
     gene            complement(3651..4208)
                     /locus_tag="VLA60_07550"
     CDS             complement(3651..4208)
                     /locus_tag="VLA60_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HSF09251.1"
                     /translation="MQGLKHFMVVAGLVTLVIGGTGSLTGADPGSAHDPHGKQPHAGM
                     GYEKSAQGMEHHGGLSPLDMKEELGLSEDQVARLRPLELDYRKTMIQNGADLRVAMVD
                     MGTLMDAKQADMAAITRKVDEISLLQKNMMMYRVGVYFKVKEVLTPVQYEQFRSRLRE
                     HMEGMAFHGGEMHEGKNPHGEYQEK"
     gene            4421..5560
                     /locus_tag="VLA60_07555"
     CDS             4421..5560
                     /locus_tag="VLA60_07555"
                     /inference="COORDINATES: protein motif:HMM:NF010639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HSF09252.1"
                     /translation="MEDFLPGLAGVPAAKSTISYVDGIQGILEYRGIPIEELATRSTF
                     TETAFLLLFNRLPSQGELSRFHEDLTHHRRIKYRITDLLKCLPEHGHPMDALQAAVAA
                     LGMFYPVPDVRDDATRYWTAVRLIAKLPTIVAAFARLRHGDEQIQPRDDLSFAENFLY
                     MLTEKVPAPDIARMFDICLILHAEHTMNASTFSGLVTASTLADPFTVIASAIGTLEGP
                     LHGGANEAVIHMLEEIGTVEAIPAYLDQKFSLKQKIMGFGHRIYKVKDPRATVLQEFA
                     QELDTRPDGNSLFALARAVEQEMEKRVGPKGIRPNVDFYSGIIYHRMGIDTDLFTPIF
                     AMARVAGWLAHCFEQYQDNHLFRPDQIYEGPRHRSYQSVETRETR"
     gene            5630..7345
                     /locus_tag="VLA60_07560"
     CDS             5630..7345
                     /locus_tag="VLA60_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HSF09253.1"
                     /translation="MNSSFSYDVLIIGAGLAGMRAAIAAHNQGASVAIMTKVHPVRSH
                     SNAAQGGINAALTDRGDHWEDHAFETVKGSDYLSDQDAVEVLAQEAGQDIIALEHMGV
                     IFNRNEEGQLGTRRFGGQKRARTFFVSDFTGQALLHVLYEQTLQAKIPVYEEWFATSL
                     VKDDQGRCAGVVAFEIRTGTFALFRAKAVIMATGGLGRVYEPSTNALICTGDGMAVAY
                     RAGAPLMDMEMVQYHPTTLKGKGLLITEAARGEGAYLLNSKGERFMERYAPNMLELAS
                     RDVVSRAEQIEINEGRGIKGCVLLDCRHLGRAFLQDRLGQIYAEAKTFANIDLAEEPL
                     PIRPGMHYQMGGIKTDTEGRCWDVQGQWAGVPGLFAAGETACVSLHGGNRLGANSLLD
                     TVVFGRRAGTYAAEYAKTLPSPTIPGSAMDADKHLVAGIVSRKGPGERAAALRLEMGT
                     TMNRHVSVFREEEGMETALHQITQLKARWPDLTIQDKGQVFNTGLIAALELGFMFDCA
                     EAIIVGALTRQESRGAHFRTDYLERDDEHWLKHVLLYHRPDAPPHVDYLPVRITQWQP
                     QIRVY"
     gene            7461..8420
                     /gene="sdhB"
                     /locus_tag="VLA60_07565"
     CDS             7461..8420
                     /gene="sdhB"
                     /locus_tag="VLA60_07565"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HSF09254.1"
                     /translation="MHTTLRIRRFNPEHDRPSSYYQEYDLEIDPSDSVLDGLIKIRET
                     IDDSLTLRCSCRGAICGSCGMRINGHATLACKTKMISVASEGGTIQVEPMNNMPVIKD
                     LVTDMAPFWSKIRQIQPWLQPEWPLPTAEYTASPESMSHLSGVMSCIMCGACVSDCTV
                     LDVDKTFVGPAALAKAYRFVADPRDAATSQRLNSLNQAGGMWDCTRCMECIQVCPKGV
                     GPMDRIMALRAKGLAEKVPATCGSRHAEVFSDIIKHKGILDEPMLAIRTFGLKNIGRL
                     LGMIPMVVQSVFKGKVPLSGPFHRPLPGIHHIQRLFKQVRIKR"
     gene            8503..9387
                     /locus_tag="VLA60_07570"
     CDS             8503..9387
                     /locus_tag="VLA60_07570"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HSF09255.1"
                     /translation="MKYAFFPGCVSKGACPELYQSVMQVYPTLGIELAEMTTASCTGA
                     GVLQEKDPRLGDALNARTFALAEQQGLPIMTICSTCQGVMSQANHRLTTQPHYLQEIN
                     GLLQEEGLTYRGTTTIKHFLWILLEDVGEETLRRHITRPLSGLRAAPFYGCYIQRPTN
                     ALEFDRHPDRGKSLERIIEILGAEVVDFPGKTRCCGFPILTINEKNSLTMVATHTRDA
                     KSHGADIMVTPCPLCHLNLDGMQTKAASQEQTSIDLPILHLPQLLGLAIGLDTKTLGL
                     QRNLISPESALAKIGGGG"
     gene            complement(9424..10395)
                     /locus_tag="VLA60_07575"
     CDS             complement(9424..10395)
                     /locus_tag="VLA60_07575"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HSF09256.1"
                     /translation="MVDVQSGQHYPSWKAFCEVRLDYVRVLAETVEQSFLRRDTTHPT
                     FCGCIDWHSSVHGAYALLTASRLTSEPRWAEIVDAALSPDCLEAELASLRNGELNHEI
                     PYGFSWFLKLAIEREQGWGKIDLLPLAAEISTQLEQWIFSLPAGEVICHLKQRNYGNL
                     SWALLNLWNWSQWKADQVLIEKLLRFTRMWVVLLDETLPPSYDNVTNEFFAASLQRTR
                     TIFTILPKEESWRWFESFCPDDLLMEPIPTAPTPHSAGLNFSRAWAFWTLFQSTRNLA
                     FRDLYVNHIVTHMDRPQYWRDDYRKHGHWVPQFGIYAIGLSFDEAPL"
     gene            10576..11010
                     /locus_tag="VLA60_07580"
     CDS             10576..11010
                     /locus_tag="VLA60_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09257.1"
                     /translation="MNFDPAIAAMHALQHAEEQGELGGLESDILEAEAIFSTDQGPQA
                     KRAFDTLQELGAQLPQARHLQEFLIYITWQQVTEGPLPRYFQLGLDLCDRFLDRFGKQ
                     IEGTPSHQHVVAIRESFQGGLGIEEEEDLMPEHDEDAFLGGD"
     gene            11284..11670
                     /locus_tag="VLA60_07585"
     CDS             11284..11670
                     /locus_tag="VLA60_07585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HSF09258.1"
                     /translation="MEVKPSGIEGRGLFTKVPLRPRQKIGEYEGERITQREGRRRAKI
                     QKWIAIVEVNNGKSIDGAAETTGFRFINHSCTPNTFMRIIGERAEFYALHPIKAGTEL
                     TLDYGDSHHNGKLPCSCGSLTCRRFL"
     gene            11893..>12524
                     /locus_tag="VLA60_07590"
     CDS             11893..>12524
                     /locus_tag="VLA60_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09259.1"
                     /translation="MVTDNSFTRSLSGLLFFTFGCVLVGQLLFKGIKGSWAESGPLKT
                     DPSQSPLPQLQRLLAEGSQNSSDPAILLHLADLYLEIGQKIYQDENRKRQAFDEGARL
                     ARRALDREEDNAEAHYLYAANLGSSAQIEGVMASALTVNTLKTHVGRALELNPRHAPA
                     LHMMGMMLEELPWFLGGDPDMAITYLIQATEVKPDYTRARLDLAKTYIKR"
BASE COUNT         3130 a         3450 c         3004 g         2940 t
ORIGIN      
        1 cccccgtctc tagacacatc aaagctccta attttcgctt gtaaccatga acaccggcat
       61 tgccaactac gggaatatct tcgtcggtcg ccagccgatt ttaggcccga atctgaagac
      121 cattggctat gaggtactgt atcgagattg tgaatccgac tccgcgaaca tccatgatga
      181 agcaatggcc acggcccagg tcctcttaaa tacctatctc gatatcggcc tagagcatgt
      241 ggtgggaacc cacctggctt ttttgaatat tcccacacaa tttttattag acagacattg
      301 tgaggcgctc ccgaaacacc gcgtggtctt ggagatattg gagacggtgg agcccaccca
      361 agaagtcatt gaagccatga cctccctctc tcagcagggc tataccattg ccctggatga
      421 ttttgtcttc catgatcgct ttcgaccgtt tctggaattg gccgatatcg taaaaatcga
      481 tgtcttagat aaaagcttcg aacaattaca acacgagacc caccagttcc gaaattaccg
      541 tgcgcgcctc ctcgccgaaa aagtggaaac acgggaggcc tttgagacct gcaaacaact
      601 tgggattttc tattttcaag ggttcttctt ttatcgccct gatattattc gcgggcacaa
      661 aatccccgcg aatcgtgtcg ccctcctgga attattagga aaaattcagg atcccaatag
      721 cacctttgaa agtctggtcg agtgtatccg aaacgatttg tctttgagct acaagattct
      781 tcgatatgtc aattcggcct ctgtcggact tcccaggcga gtggaatcca ttgagcaagc
      841 tgcctgtatg gtaggcctcg accggatacg cacctgggcc tccctcattg taatggccat
      901 tggaaaggac cagccgatgg aaatgctggt gattgccttg gtgcgagcga aaatgtgcga
      961 gagcttagga cagcagcttg gcgccgattc tccagaaaag tattttacca tgggccttct
     1021 gtcggtctta gaagccttgt acggatcttc tatggaaaaa cttgtgggga accttccgct
     1081 cccggacgat attcttgaag ccttaatact ggagaaggga acaatgggaa ccgcgttgag
     1141 tagcgtgaaa gcctacgaaa agggggagtg gttgcaactc aaaacactcc aactctcccc
     1201 tggcacaatt cgggatttct acgtgcaagc cattgattgg gccaaccact tctctcctat
     1261 gattgaagag cccgcatgac agtctgacaa aaccgcccat ttgctcacct tcgcttactt
     1321 cagccctcat cccttcaaga ttcccctcgc tccttgactc tctggcagat cttttcgaga
     1381 aagtaggcat ccctatattc acgtgttaga atacgacatg cccattgcct gggtgcccaa
     1441 ctaaatgaca tctcagaaac acattcacag gtcaccataa aactcaaaga catccatgac
     1501 attcatccct acttcagaaa tcactcccgt ctcttcttcc ggaaagacct cccgtaggag
     1561 aaacctccct tcctggttca aagtccgttt ccggccgggc ccccattacc aggaaatccg
     1621 tcaactgatg gatacgcatc gtctgcatac catttgtgaa gaagcccgat gtccgaatat
     1681 atgggaatgt tggaataatc ggaccgccac gttccttatc cttggtgata tttgcaccag
     1741 gcggtgtcat tactgttcgg tcaccaccgg acgcccgtcc gcagtggacc gagaagagcc
     1801 tcagcgcgtg gcagatgccg tcaagctcct taaactgcgg catgcggtgg tgacatcggt
     1861 caatcgagac gatctccccg atggaggggc cgcactgttt gccgagacca ttcaacgaat
     1921 ccgccatgaa aatcccgcat gcaccgtcga agtattgatc tcggactttc agggacatca
     1981 gactgatctg gaaatagtca tgcaggccaa gccggatatt ttgaaccata acattgagag
     2041 cgttcctcgc ctcttccctt ccattcgtcc acagggaaaa taccaacgct cgctccaggt
     2101 ccttgattgg gccaaagcca tgggaggacg aaccaaatcc gggctcatgg caggtttggg
     2161 agaatcaaac gatgaaattc ggcaggtgat gcaggatctt cgaaaagtgg attgtgacat
     2221 tctgaccatc ggccaatacc ttcagccgac tcttcagcat gcgccggttt ctcgatacta
     2281 tagtccggag gaatttgagg aattcaaaca ggagggaacg gctttaggtt ttcagcacgt
     2341 agaatccggc cccctcgtcc ggagttctta ccatgcagaa gagcaaacac acggacaaac
     2401 ctgtccctga aatccattcc ctctgtatgc cccaagccat tctatctgcg gggccgcagg
     2461 cgcttgtcag accggttggt gaagatctaa tcgggaaaca ggcttgtctg acttggcata
     2521 cgagggattc tgcttctgtg cgagggaatc caaatacaca tgttcacaga ggttccgcag
     2581 agtgacgaaa tcaaattgcg tatattcttg acagcaggtt tcacatttca tcattggact
     2641 tctctccaat aatttgctaa catgcaccca ataaattaaa attagcggaa ggacgaacac
     2701 ttcgaatcac acaccgtgac tcccccaagt gacaaagtga ataattctga aatgtccacc
     2761 cttcccgcat tcatgacatc gtggtgtcct tctcttatct ccttccgcga tgccctcttc
     2821 ccataaaatt ctttcttacc gtttcagtct caggcaacga aataccgcgc ttcctgttat
     2881 cgaatgatca acgcatgtcc gtccggggcg gagaccaggg cattcacggg ttgcccgagt
     2941 tctttttgcc cattgggaaa ggcggtatct ttccccacct ggattttaat ctgtttttcg
     3001 gcagacgccc cttcaatgat aaacaggtct ccctgaatag cctgaagcgt cccttccaca
     3061 ttgacatacc gtgcggtcga agtcggcgaa ttcgtctgta gttcctgccg aacctgaggt
     3121 gtttgggaca tctgttcccc ctccatggca agccccgaga gaactccctg gaaacaaccc
     3181 attaccacca tcaaaataaa aagtttggtc aatggtttca tttcaacctc cttccacgac
     3241 aaataaatgc gaccgaggac cttatcgtgt ttcgttgctg atataaaaaa gacattgcaa
     3301 ggctcattcc ctaccatgac atcatttttt tcatgaacaa catcaagtgg ttagatgtct
     3361 cctgtggcaa accgcaacag gatacacatg aggagatgtg caggcaatcg cctacaactt
     3421 caccaacaaa cctgtaggga gataaatgca gattggcaga aatctgaaaa acgaggggcc
     3481 ccttctctta ttacgaagaa ggggcccttt tgaaggatga cttccacggg atgctcaagg
     3541 cgcccagacc tcaatacgtc ctctcgggtc caaaaaacga aagaaatctc ctcaagagga
     3601 acttttcatg atcagggatg gtttttctct atcccaccat caggaccatg ctatttttct
     3661 tgatactctc catgagggtt tttgccttca tgcatctccc ctccatgaaa ggccattcct
     3721 tccatatgct cacgaagccg ggaccgaaac tgctcatatt gcacgggcgt caagacctct
     3781 ttgactttga aataaacccc gacacgatac atcatcatgt ttttttgtaa taggctgatt
     3841 tcatcaacct tacgggtaat ggcggccata tcggcttgtt tcgcatccat aagggtcccc
     3901 atatcgacca ttgccacccg caaatcggcc ccattctgaa tcatggtctt ccgatagtcc
     3961 aattcaagcg gacggagccg ggccacctga tcctcagaaa gacccagttc ctctttcatg
     4021 tccaatggag aaagaccacc atgatgctcc atcccttgag cacttttttc atatcccatc
     4081 cccgcatgtg gctgtttccc atgtggatcg tgagcactcc cggggtcggc tcctgtcaag
     4141 gatcctgttc cccctatgac aagtgtcacc aaccctgcaa cgaccatgaa gtgttttaaa
     4201 ccctgcatac tcgaatcccc tttcttgaaa gtaaaatgac tcacctttcc gagaccagtg
     4261 actcatttct aatttatagt caacaccgga tttcataata atttcctcaa ttaactatac
     4321 caaattcatc gggaaagttt ggggggaaga tcatgagcaa ggtatactag aattatatga
     4381 cattattcgg agatgacctc taagcgaaag acaaaatgac atggaagatt ttttgccagg
     4441 cctagcaggg gtccctgctg cgaaatcaac aatcagttat gtcgatggaa tccaggggat
     4501 tctagaatat cgcggaatcc cgattgagga acttgcaacc cgaagcacct tcaccgaaac
     4561 ggcgttttta ttattattca accggcttcc atcccaggga gaactctccc ggtttcatga
     4621 ggatcttact caccatcgcc ggatcaaata tcgcatcacc gatcttctga aatgcctccc
     4681 ggaacatgga cacccgatgg atgcccttca agcggcagtg gccgccctgg gcatgtttta
     4741 tccggtgccg gatgtccgtg atgatgccac ccggtactgg actgcggtcc ggctcatcgc
     4801 caaactccct accatcgtcg ccgcgtttgc ccgcctgcgt catggtgatg agcagataca
     4861 accgcgtgac gacctttcct tcgccgaaaa ctttttgtac atgctcacag aaaaagttcc
     4921 ggctcccgac attgccagga tgtttgatat ctgtttgatt ctccatgccg aacacaccat
     4981 gaatgcctcg acatttagcg gactggtgac ggcctccaca ctggccgatc cgtttaccgt
     5041 aatcgcctct gccattggca ccctagaagg gcccttacat gggggcgcca atgaggcagt
     5101 cattcacatg ctggaggaaa taggaaccgt tgaggctatt cctgcatatc tggaccagaa
     5161 gttttcatta aagcaaaaaa ttatggggtt cgggcatcga atttataaag tcaaagaccc
     5221 acgcgccacc gtgttacaag agttcgccca agaattggat acccgaccgg atggcaattc
     5281 cttatttgct ctggcccggg cggttgagca ggaaatggaa aaacgcgtgg gcccaaaagg
     5341 catacggccc aacgtggatt tttactccgg gattatttac catcgaatgg gcatcgacac
     5401 ggatttattc actccgatct ttgcgatggc cagggtggcc gggtggttag cgcactgctt
     5461 tgaacaatac caggataatc acttattccg gcctgaccag atttatgagg ggccccgcca
     5521 tcgttcctat caatcagtgg aaacccgtga aacccgttaa tcgggtcaca aacccgaccg
     5581 acgtcatcgc tgtgagaatg tgtgtgccac gtattgttgc ttgagaatca tgaactcttc
     5641 cttttcttat gatgtgctca tcattggggc cggtctggcg ggaatgcggg cggctattgc
     5701 cgcgcataac caaggagctt ctgtggccat catgacaaaa gtccatcctg tgcgcagtca
     5761 ttccaatgcc gcgcaaggcg gcattaacgc cgccctgacc gaccggggag accactggga
     5821 agaccatgcc ttcgaaacgg tgaaaggcag tgattatctg agcgaccagg acgccgttga
     5881 ggtcctcgct caggaagccg gccaggatat tatcgccttg gagcatatgg gggtcatctt
     5941 taaccgaaac gaggaaggcc agctcgggac ccgtcgattc ggtggacaaa aacgcgcacg
     6001 aacttttttt gtgtccgact ttaccgggca ggcgctgctt cacgtgctgt atgaacaaac
     6061 gctgcaggca aaaattcccg tctatgaaga atggtttgcg acctccctcg tgaaagacga
     6121 ccagggacgg tgtgccggag tagtggcctt tgaaattcgc acaggaacct tcgcgctatt
     6181 tcgagccaaa gccgtgatca tggccacagg cggcttaggc cgggtgtacg aacccagcac
     6241 caatgccttg atttgtacgg gtgatggcat ggccgtggcc taccgtgccg gggcgccttt
     6301 aatggatatg gaaatggtgc aatatcaccc gacgacgctg aaggggaagg gcctcttgat
     6361 tacagaagcc gccagaggag aaggagccta tcttctcaac agcaaggggg agcgcttcat
     6421 ggaacgctat gcgcccaata tgctggagct ggcttcacgg gatgtcgtct cccgggcgga
     6481 acaaattgaa atcaatgaag ggcgtggcat caaaggctgc gtcttgctcg actgccggca
     6541 tttgggacgg gcctttcttc aggatcgtct cggacaaatc tatgccgaag ccaaaacgtt
     6601 tgccaatatc gatttagccg aagagccgtt acccatccga cccggcatgc attaccaaat
     6661 ggggggcatc aaaaccgata ccgaaggccg atgttgggat gtccagggcc aatgggccgg
     6721 cgtgccaggc ctcttcgctg cgggagaaac tgcctgcgtg agtttgcacg gcggcaacag
     6781 gcttggcgcg aactctctgt tggacaccgt ggtatttggc cgacgggcag gaacatatgc
     6841 ggcggagtat gccaagacac tcccttctcc caccattcca ggcagcgcga tggacgcaga
     6901 caaacacctg gtggccggca tcgtgtcacg gaaaggaccg ggagaacgag cggctgcgtt
     6961 acgcctggag atgggcacca ccatgaaccg gcatgtgtcg gtctttcgtg aggaggaagg
     7021 catggagacc gccctccacc aaatcaccca attaaaagca cgttggcccg acctcaccat
     7081 tcaggataaa gggcaggtgt ttaataccgg attgatcgcg gcactggaac tggggtttat
     7141 gtttgactgt gcggaagcca ttattgtggg cgcgctcacc agacaggaaa gccgtggggc
     7201 ccatttccgg accgattatc ttgagcggga cgacgaacac tggctcaaac atgtgttgtt
     7261 gtatcatcga cccgatgctc ctccacacgt cgactatcta ccggtgcgga tcacccaatg
     7321 gcaacctcaa attcgcgtct attaatgaag gcaatgggcc catcatggcc tcttcggaac
     7381 cgtctgaggg atgcggagcc acccggtaac ggcttgaagg aggtatactg tacacataag
     7441 ccgaggaatc atccccactg atgcacacca ctcttcgcat acgccgcttt aatccggaac
     7501 acgatcgtcc ctcctcgtat taccaggaat atgatctgga aattgatcct tccgacagtg
     7561 tcctcgatgg actcattaaa attcgagaga ccatcgatga ttccttaacc cttcgttgtt
     7621 cctgtcgcgg agccatttgc ggatcctgcg gcatgcgcat taacgggcac gccacactgg
     7681 cctgtaaaac caagatgatt tcggtcgcct cggaaggagg gacgattcag gttgaaccca
     7741 tgaataacat gccggtcatt aaagatctgg tcacggatat ggcgccgttc tggtcaaaga
     7801 ttcgacagat tcagccgtgg ctccaaccgg aatggccgct tcccaccgct gaatacaccg
     7861 cctccccgga atccatgagc cacctctccg gggtcatgag ctgtatcatg tgtggcgcct
     7921 gcgtctccga ctgcacggta ttggatgtcg ataagacgtt cgtcgggcct gctgccttgg
     7981 caaaagccta ccggttcgtc gccgacccgc gtgatgcggc cacttcccaa cgactgaatt
     8041 cccttaatca ggcgggcggc atgtgggatt gcacccggtg tatggaatgt attcaggtct
     8101 gcccaaaagg ggtaggcccc atggaccgca tcatggcgtt gcgggcaaaa ggcctggcag
     8161 aaaaagttcc cgccacctgt ggctcccgac atgccgaagt cttttcggac atcatcaaac
     8221 acaaagggat tcttgacgag ccgatgctgg cgatcaggac cttcggcctt aaaaatattg
     8281 gccgattact cggtatgatc cccatggttg ttcaatcggt cttcaaaggc aaagtccccc
     8341 tctcgggtcc gtttcaccgc ccgcttcccg gaatccacca tatccaacgg ctctttaaac
     8401 aggtcaggat taaacgatga catctgccaa acccattacc tttatttccg ggcacaaagc
     8461 ttttctccct ctacgaggga gaaaaccaga agggagagag caatgaaata tgccttcttt
     8521 cccggttgcg tctcaaaggg agcctgccca gaattatatc aatccgtcat gcaggtctat
     8581 cccacattgg gaatcgaact cgcggaaatg acgaccgcct cctgtaccgg tgcgggtgtg
     8641 ttgcaggaaa aagacccgcg gctgggcgat gcgctgaatg cccgcacttt tgcgctggct
     8701 gaacaacaag gtctccccat catgaccatc tgtagtacct gccagggcgt gatgagccag
     8761 gccaatcatc gtctcaccac tcaaccgcac tatcttcagg aaattaacgg cttgctccaa
     8821 gaggaaggat taacctatcg gggaacaacc acgatcaaac attttttgtg gattctcctg
     8881 gaagacgtgg gcgaagaaac cttgcggcga cacatcaccc gtcccttatc gggtcttcgt
     8941 gccgcgccgt tttatgggtg ctatattcaa cggccgacca atgcgctgga attcgaccgg
     9001 catcctgatc ggggaaagtc ccttgaacga atcatcgaaa tattaggagc ggaggtcgtg
     9061 gactttccag ggaaaacccg atgttgcggg tttcccattt tgaccattaa tgaaaaaaac
     9121 tcgcttacca tggtcgccac gcatacgcgg gatgccaaat cacacggagc cgatatcatg
     9181 gtgaccccct gcccgctctg ccatctcaac ctcgacggca tgcagaccaa agccgccagc
     9241 caggaacaga cttctatcga tctcccgatt ctccatcttc cgcaattatt aggccttgcc
     9301 atcgggttgg ataccaagac cctcggatta caacgaaacc tcatttcccc tgaatcggct
     9361 ctggccaaaa tcggtggcgg cggataacca cagttttcct gataataaga aaggtctgca
     9421 gtgttagagc ggcgcctcat cgaagcttaa cccaatggcg tagatcccga attggggaac
     9481 ccaatgcccg tgttttcgat agtcatcccg ccaatactgc ggcctatcca tatgcgtcac
     9541 aatatgattg acgtacaagt cacgaaatgc caagttccga gtggattgaa agagcgtcca
     9601 gaatgcccaa gctcggctaa agttgagtcc tgcggaatgt ggcgtgggag ctgtggggat
     9661 cggttccatc aaaaggtcgt ctggacaaaa tgactcaaac catcgccaag attcttcttt
     9721 gggaagaatg gtaaaaatgg ttctggtccg ttgcagagag gccgcaaaaa actcattcgt
     9781 cacgttgtca tatgaaggag gcaatgtctc atcaagaagg acaacccaca ttcgtgtaaa
     9841 cctgagaagt ttttcgatta aaacttggtc agccttccat tgactccaat tccacaaatt
     9901 cagcaaggcc catgacaaat taccatagtt cctctgtttc aaatgacaaa taacctctcc
     9961 agcgggaagg gaaaaaatcc attgttccaa ctgtgtggaa atttctgccg ccaacggtaa
    10021 caagtctatc ttcccccacc cctgctcccg ttctatggcc agtttcaaaa accaagaaaa
    10081 gccatagggt atttcatgat tgagttcccc gttcctgagt gaggccaatt ccgcctccag
    10141 acaatcaggc gagagtgccg catcgactat ttcagcccac cggggttcac tcgtcagccg
    10201 cgaagccgtt aataacgcgt aggcgccatg gacactggaa tgccaatcga tacacccaca
    10261 gaaggtcgga tgagtcgtat cccgccggag aaaactctgt tccaccgtct cagccaaaac
    10321 ccgaacatag tccagccgga cctcgcagaa cgccttccac gacggatagt gttgaccgga
    10381 ttgtacatcc accattgagc cccttccctc cttacatggt aacctacatc acaccatcat
    10441 ggctcagcta gagagcctga atcctataaa gattgcattt cccgttgtca gattttggat
    10501 acgcaaactc gtcggaaatc tgctacactc cctcgacgtt cagatacctc atgttcacag
    10561 acaaaggcgt ttcccatgaa ttttgaccca gccatcgctg ccatgcacgc actgcaacac
    10621 gctgaggagc aaggagaact tggcggtctt gaatcggaca ttttagaagc cgaggcgata
    10681 ttttccaccg atcaaggacc acaagccaag cgcgcgtttg atacccttca ggaattgggc
    10741 gcacagcttc cacaagctcg acacctgcag gagttcctca tttacattac ctggcagcaa
    10801 gtcaccgaag gccccctgcc ccgatatttt caacttgggc tggacctctg cgatcggttc
    10861 ctggatcgat tcggaaagca aattgaaggc actccctcac atcaacacgt ggtcgccatc
    10921 cgggaatcgt ttcagggcgg attaggcatc gaggaagaag aggaccttat gccggaacat
    10981 gacgaggatg cgttcctggg cggtgattga ccttgagaca tcccctgacc cgccggtatc
    11041 tcccagaata atcagacctc ctgcgggttc gtctgatcga atgaatgagc ttcgctggaa
    11101 aacacgtgcc gtctctcctt tcccttcatc tctcctctat ccttgcctca ccaacttccc
    11161 attttgtaca gttccctgaa atccgtttcc ctaaaaatat tccaacgtat tgcttcttgc
    11221 cccactcttc gaatgccccc aactaatcgg gcttcggccc gcaaaaccag atggcccgtt
    11281 ccgatggaag tcaaacccag tggtattgag ggccgcggcc tattcaccaa agtgccattg
    11341 cgacccagac aaaaaatcgg agagtatgaa ggggaacgca ttacgcaacg agaaggacga
    11401 aggcgcgcca aaatccaaaa gtggatagcc atcgtcgaag tgaataatgg gaagtcgatc
    11461 gatggggcag cagagacaac aggattccgg ttcatcaacc attcctgcac ccccaatacc
    11521 tttatgcgca tcataggtga acgggcagag ttttatgcct tgcacccgat caaggccgga
    11581 accgaactca cgctcgacta tggcgactcg caccataacg gaaaactgcc ctgtagttgt
    11641 ggaagcctaa catgtcgaag atttctttag aaaagcgcat acgctttcat tgcaaataca
    11701 taccgactcg cttccctcgg cctacgccgt tcagctcaag atcttttgga ttcgactttt
    11761 cgcgttccat cagccaccat ccatcctttt ttatccctat ccaggaccac ttccgcattt
    11821 tacctgtgct agaatccaga tccctggcgg gtcttcttgc tcattgaaat ttcatgaacc
    11881 acaccgaaaa ccatggtcac tgacaattct tttacgcgaa gcctcagtgg cctccttttt
    11941 tttactttcg gatgcgtgct tgtcggacag ttgctgttca aggggattaa ggggagttgg
    12001 gcggaatctg gtcccctcaa aaccgatcct tcgcaatccc ctttgcccca actccaaaga
    12061 ctcctggccg agggcagtca aaattcttca gacccggcca tactcttaca cttagccgac
    12121 ctgtatctcg aaattgggca gaaaatctat caggacgaaa accggaaacg tcaggcattt
    12181 gatgaagggg cacgtctggc ccgacgggcc cttgatagag aagaagacaa tgcggaagcg
    12241 cattatttat acgcggcgaa tctgggaagc tctgcccaaa tcgaaggtgt catggcctcc
    12301 gcgctgacag tcaacacgtt gaagactcat gtgggaagag ccttagaact caacccccgc
    12361 cacgcaccgg cacttcacat gatgggtatg atgctggagg aactgccgtg gtttctcggc
    12421 ggtgaccccg acatggccat cacgtacctg attcaggcca cggaggtgaa acccgattac
    12481 acgcgagcga gactggattt ggctaaaacc tatatcaaaa ggca
//
LOCUS       DATIAL010000244         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_475084, whole genome shotgun sequence.
ACCESSION   DATIAL010000244 DATIAL010000000
VERSION     DATIAL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475084"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VLA60_07595"
     CDS             <1..250
                     /locus_tag="VLA60_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSF09260.1"
                     /translation="TEGEDKPLKYPHMFKNATTMILNKIDLLPYLTFDVAKCLEFAHM
                     VNPGLQVFQVSATTGEGLQHWYDWVTAQINASNRQGVR"
     gene            250..918
                     /locus_tag="VLA60_07600"
     CDS             250..918
                     /locus_tag="VLA60_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09261.1"
                     /translation="MVSVLGLGFLVGMRHAIEADHAAAIATLATKNYSVANTLKQGLT
                     WGLGHTITLLLFGSMVFLLEAAVPERLANWLELAVGVMLVGLGSDVLRKIIQGRIHIH
                     VHQHLHQPPQLHAHSHGGEINHQMTDHHHSHSTAFPYRALFVGLMHGMAGSAALILLT
                     LQQTVSPLQGIIYIATFGLGSMLGMATLSMVIAIPLRYSSRSIRWLTTGCRSPSARSR
                     LSLG"
     gene            complement(1080..2966)
                     /gene="acs"
                     /locus_tag="VLA60_07605"
     CDS             complement(1080..2966)
                     /gene="acs"
                     /locus_tag="VLA60_07605"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HSF09262.1"
                     /translation="MSENIETLQRSTRVIPPPPDVTATAHIQDYESAYKQSIANPEKF
                     WDGIAKELHWFSPWNKVLEWNYPWAKWFVGGTCNIAYNCLDRHVQTWRKNKVAVIWVG
                     EEGQERILTYGELYRQVNKCANALKSLGLNKGDRVTIYLPKIPEQIVAMLACARIGVI
                     HTVVYSGFSATALAARIEACKARVVITADVGYDRSRKIPLKPVVDEAVAKCQTVEKVI
                     VVQREHSSSPLQAPKELDWEAWLKDQSPKCDAEQLDSETPLYILYTSGTTGKPKGVVH
                     VHGGYMVGTYITTKYVFDLKDEDVYFCVADPGWVTGHSYIVYGPLLNGATILTAEGKP
                     DYPNPGRWWDLIERYGVSIFYTTPTAIRLLMKFGEDWPKKYDLSSLKVLGSVGEPINP
                     EAWEWFHRVTGGTKPIMDTWWQTETGMIMVTPLPCVPLKPGSATRPFLGIEADVMDRE
                     GNTVEHGGGFAVIKKPWPSMMRTIYNEPDRYLLYWNTIPGVYTAGDVCHKDEDGYLWF
                     MGRADDVVKVAGNRIGTAEVESALVSHEAVVEAAVIGKPHPTAGEQIKAFVILKQGQQ
                     ESPDLIKSLQDHVLQELGKIAVPREIEIVPSLPKTRSGKIMRRVLKAKELGQDPGDIS
                     TLEE"
BASE COUNT          715 a          820 c          842 g          825 t
ORIGIN      
        1 cactgaaggc gaagacaagc ccttaaagta tccgcacatg tttaagaatg cgaccacgat
       61 gattctgaat aagatcgatc ttcttcccta tctcaccttt gatgtagcaa agtgcctgga
      121 atttgcgcat atggtgaatc cgggtcttca ggtgtttcag gtgtcggcca ccaccggtga
      181 gggacttcaa cactggtatg actgggtgac tgcacaaatc aacgcttcaa accggcaggg
      241 tgttcgctaa tggtcagtgt attggggctc gggtttctgg tgggaatgag gcatgcgatc
      301 gaggccgatc acgcggcggc gattgccaca ctcgcgacca aaaactattc ggtggcgaac
      361 acgctcaagc agggattgac ctgggggcta gggcatacga tcacgcttct gttgtttggc
      421 tcgatggtat ttcttctgga agctgcggtt cccgaacgac tcgccaactg gctggaattg
      481 gccgtggggg tcatgctggt aggactggga agcgatgtgt taaggaaaat catacaaggt
      541 cgcattcata ttcatgttca ccaacacctt catcaacctc ctcaactgca tgcgcattcc
      601 catgggggag aaatcaatca ccaaatgacc gaccatcacc attcacattc aaccgctttt
      661 ccctaccggg ccctattcgt aggcttgatg cacggaatgg cagggtccgc tgcgctcatt
      721 ctgctgacac ttcaacaaac cgtttctccg cttcaaggga ttatctacat tgcgacattc
      781 ggtcttgggt caatgctggg aatggccacc ctctcaatgg tcatcgccat ccccctccgc
      841 tattcctccc ggtcaatacg ttggctcaca acgggctgca ggtctccatc ggcgcgctca
      901 cgattgtcct tgggctgatg ctgatttata cgacggactt cagcctcttg agttaaactt
      961 tcggtccttc ccaaaaatcg gacattgttc tctcctgacc gtaggttcaa ttttttttga
     1021 gcttcagcag gctcaggtaa aaggatttaa tctaagaata tgtaatccgt aatcaggatt
     1081 tactcctcga gtgttgagat gtctccagga tcctgtccga gctccttggc ctttaggacg
     1141 cggcgcatga ttttgccgga acgggttttc ggaagcgacg ggacaatttc aatttcccgg
     1201 gggacggcga tcttgcccag ttcctgtaag acatgatcct gcaagctttt gatgagatcg
     1261 ggtgattcct gttggccctg cttcaggatg acaaaggcct tgatttgttc tccggcagtt
     1321 ggatggggtt tgccgatgac ggctgcttcc accaccgctt catggctgac taaggcgctt
     1381 tcgacttcag cggtaccgat ccgatttccg gcgactttca cgacatcatc cgctcgcccc
     1441 ataaaccaca agtagccgtc ctcatcctta tggcacacat cgccggcggt atacacaccg
     1501 gggatggtat tccaatataa gagatagcga tccggctcgt tgtagatggt gcgcatcatc
     1561 gatggccagg gctttttaat gacggcaaaa ccaccgccat gctctaccgt attgccttcg
     1621 cggtccatca catccgcttc gatgcctaaa aaaggacggg tggctgagcc tggtttgagt
     1681 ggcacgcagg ggaggggggt gaccatgatc atgccggttt ccgtctgcca ccaggtatcc
     1741 atgatgggtt tggtgccacc ggtcacccga tgaaaccatt cccaggcttc cggattgatc
     1801 ggttcaccca ccgaccctaa aaccttgagg gaggagagat cgtatttttt cggccagtct
     1861 tctccaaatt tcatgagcag ccggatggcc gttggagtgg tatagaaaat cgagacgcca
     1921 tagcgttcaa tgagatccca ccagcgcccg ggattgggat agtcgggttt gccttccgcc
     1981 gttaaaatcg tggctccgtt taagagtggt ccatagacga tataactatg gccggtgacc
     2041 caccccggat ccgccacaca aaaatagaca tcttcgtctt taaggtcgaa gacatatttg
     2101 gtcgtgatgt aggtgcccac catataaccg ccatgaacgt gaacgacgcc tttaggtttc
     2161 cccgtggttc ctgaggtata gaggatgtag agcggggttt cggaatcaag ctgttcggca
     2221 tcacactttg gagattggtc tttgagccag gcttcccaat ccaattcttt cggggcttga
     2281 agaggggagg atgagtgttc ccgctggacc acaatgactt tttccacggt ctggcatttg
     2341 gcaaccgctt catcgacgac cggcttgagg gggattttcc gactgcggtc ataccccaca
     2401 tccgcggtga tgaccacccg cgctttgcat gcctctatgc gtgccgctaa cgctgtggca
     2461 ctaaaacccg aatacaccac cgtatggatc actccgatgc gagcacaagc caacatggcc
     2521 acaatttgct cggggatttt cgggagatag atggtcaccc ggtcaccctt gttgaggccc
     2581 aggcttttca gggcattggc gcacttgttg acctgccggt ataactcgcc ataagtcagg
     2641 attcgttcct ggccttcttc tccgacccag atgacggcca ctttgttttt tcgccaggtt
     2701 tggacatggc gatcgagaca attgtaggca atattacagg tgcctcccac gaaccatttg
     2761 gcccagggat agttccactc caggacctta ttccaggggg aaaaccagtg gagttccttg
     2821 gcaatgccgt cccagaattt ttcaggattc gcaatggact gcttgtaggc gctttcataa
     2881 tcttgaatat gggcagtggc tgtgacgtcg ggtgggggag gaatgacccg ggttgatcgt
     2941 tgcagtgttt caatattttc actcattagg tttgttcctc cttagttacg catttaggac
     3001 aacatcaaga acatattttc ttagcgagaa ggctgataag cccattatgc tgtccttggc
     3061 tcgttgcttc aagagggcgg agattaaaga ccgtgaaaaa gaggtgagca ttgaataggg
     3121 tttggaggct atgaacagga gaatcgtcgc tgaccgcaag ccgttgaaac ttttatttca
     3181 aatggcattg agatgaatga ga
//
LOCUS       DATIAL010000245         2369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1227655, whole genome shotgun sequence.
ACCESSION   DATIAL010000245 DATIAL010000000
VERSION     DATIAL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227655"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1914
                     /locus_tag="VLA60_07610"
     CDS             61..1914
                     /locus_tag="VLA60_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSF09263.1"
                     /translation="MTEKSLQQINTSALRDRTFFPSPTAWEDQVLYFLMLDRFSDGQE
                     KGYRSNDGTVVRRGSTPLFQSTDAGNADASHWKAAGQKFCGGNLSGLTSKLGYIERLG
                     VTAIWISPIFKQVKFKDTYHGYGIQNFLDVDPHFGQREDLRTLVRTAHAHGIYVILDI
                     ILNHTGDVFRYNPNRYRTEHNGQVFNDPRWDGQPYDVQGFHDPFGEPALPFQQLDIAS
                     HPAAWPHGAIWPQEFQDPQAFTRKGHITNWDFSPEFLEGDFLDLKNIQLGSGDVDQYQ
                     PSEALKALAEVYKFWIAFADVDGYRVDTVKHMDLGASRYFASVIHEFTQSIGKENFYL
                     IGEITGGRTRAFQTLETTGLDAALGVDDIPDKMEYLVKGYRNPDEYFQLFRNSILVQK
                     ESHVWFRNRVVTLFDDHDQVRKGENKARFCADPAGPDGLLNVLALNALTMGIPCMYYG
                     SEQYFDGHGQSDQYIRECMFGGEFGAFQSHQRHFFNEESPAYRQLSILLGIRRNKLAL
                     RRGRQYLREISGNGIDFGLPRMLGGQIRSVIPWSRIFNDQEILLAMNTDYAAPRSAWV
                     TIDDGLHHTGQTLTCLYSTNAGQIGQETTVESRNGKAVVLTLPAGGFVIYE"
     gene            complement(1941..>2369)
                     /locus_tag="VLA60_07615"
     CDS             complement(1941..>2369)
                     /locus_tag="VLA60_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HSF09264.1"
                     /translation="KPDEKVLEIGTGSGYQAAILGELVSKVFTIEIVEPLAAEAKKTL
                     AELGYDNVIVRAGDGYQGWPDESPFDAIILTAAPNHIPEPLLEQLAIGGRLILPVGDY
                     PQRLLLIRRTEEGYQETELLPVVFVPMTGEALTGPPETTP"
BASE COUNT          572 a          637 c          605 g          555 t
ORIGIN      
        1 agaaccggaa tgagggttca cccacacgat tttctgaaga gctaaaaaaa ggagtctttc
       61 gtgacagaaa agagtcttca acaaatcaat acctctgctc tccgtgaccg gacgtttttt
      121 ccttctccca ccgcatggga ggatcaggtc ttatactttt taatgctcga ccgtttttcg
      181 gacggacagg aaaaaggcta cagaagcaat gacggaaccg tcgtccggcg tggctcgact
      241 cccctctttc agtcaaccga tgccggcaat gcggatgcat ctcattggaa ggcagcgggg
      301 cagaagtttt gcggagggaa tttatccgga ttgacgagca agctgggata cattgaacgt
      361 ctcggcgtga ccgccatttg gatcagtccc attttcaaac aggtgaaatt taaagacacc
      421 tatcatggat atggcattca aaactttttg gatgtcgacc cgcattttgg tcaacgagaa
      481 gatctgagaa cccttgtccg gaccgctcac gctcacggga tctacgttat tcttgatatt
      541 atcctcaacc acacgggaga tgtgtttcgc tacaatccca accgctatcg gaccgaacat
      601 aacgggcagg tcttcaacga tcctcgctgg gatggccaac cctatgacgt gcaagggttc
      661 catgatccat tcggagagcc ggctctcccc ttccaacaac tcgacatcgc aagccatcct
      721 gccgcctggc cgcatggagc catctggcca caggaattcc aagaccctca ggcctttacc
      781 cgaaaaggcc atatcaccaa ttgggatttt tcccctgaat ttcttgaggg ggatttcctt
      841 gacctgaaaa atatccaact cggttccggc gacgtggatc agtatcagcc gtcggaggcc
      901 cttaaggccc tggccgaggt gtacaaattc tggattgcct ttgcggacgt cgacgggtat
      961 cgggtggata ccgtcaaaca tatggacctg ggcgcgagcc ggtactttgc ctcggtgatc
     1021 catgaattca cccaatccat cggcaaggaa aatttctatc tcattggaga gattaccggg
     1081 ggcagaaccc gcgcgtttca aacgctggaa accacgggcc tggacgccgc acttggcgtc
     1141 gacgacatcc cggacaaaat ggaatacctc gtgaagggct atcgcaatcc ggatgagtat
     1201 ttccagctat tccggaattc catcctggtt cagaaagaat cccacgtctg gtttcgcaac
     1261 cgggtggtga ccctgtttga tgatcatgat caagtacgaa agggagaaaa caaagccaga
     1321 ttttgtgctg atcctgccgg accggacgga ctcttgaatg tccttgcgct gaacgccctc
     1381 acgatgggca tcccctgcat gtactatggc agcgaacagt attttgacgg gcacgggcag
     1441 agtgatcaat acattcgcga atgtatgttc ggaggagaat tcggcgcctt tcaaagccat
     1501 caacggcatt ttttcaatga agaaagtccg gcataccggc aactttcgat cctattaggc
     1561 attcgccgta acaagcttgc gttacgacgg gggcgacaat acctgagaga gatttccgga
     1621 aatgggatcg acttcgggct gccgcgcatg ctgggtgggc agattcgttc cgtgattccc
     1681 tggtccagga ttttcaatga ccaggaaatt ctcctggcga tgaataccga ctatgctgcc
     1741 ccccgaagcg cctgggtgac gattgatgac ggactgcatc acaccggcca aacactcacc
     1801 tgcctctatt caaccaatgc cggacagatc ggtcaggaga cgaccgttga gtcgagaaat
     1861 ggtaaagcag tcgtgctcac ccttccggcg ggcggattcg tcatctatga ataattgatg
     1921 cagacaactg cagattgaca tcagggtgtg gtttccggag gacccgttaa ggcttcgccc
     1981 gtcatgggca caaacaccac cggtaacaat tcggtttcct gatatccctc ctcggtgcga
     2041 cgaattaaca gaagacgttg agggtagtct ccgacgggaa gaatgagacg tccgccgatt
     2101 gccagctgct ctaagagtgg ttcgggaata tggttcggcg ccgccgtcag aatgatcgca
     2161 tcaaacgggc tttcgtccgg ccatccttga tagccatcac cagcccgtac aatcacattg
     2221 tcatatccaa gttctgccag ggtctttttt gcctccgccg cgagtggttc gactatttct
     2281 atggtgaaca ctttggaaac caactccccc agaatggcag cctggtaccc tgatcctgtc
     2341 cctatctcaa ggaccttttc atccggctt
//
LOCUS       DATIAL010000246         6311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_690184, whole genome shotgun sequence.
ACCESSION   DATIAL010000246 DATIAL010000000
VERSION     DATIAL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690184"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1264)
                     /gene="alr"
                     /locus_tag="VLA60_07620"
     CDS             complement(41..1264)
                     /gene="alr"
                     /locus_tag="VLA60_07620"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HSF09265.1"
                     /translation="MIPIRPPSPSDLRHVSSPITVSISLDALTRNFQQIRQWVAPSTK
                     ILAIIKADAYGHGAIPVAQTLEHLGIFGFGVASVMEGITLREHHIHCPILIMGPILPQ
                     HLSEIIRHQLTPVISRAEIVQQLIELLFPRATPFPIHLKIDTGLHRLGFENEEALSLL
                     SRLSLSTPLIEIEGLLSHFADADNQDHTLTNVQIQKFLAFIDQAKQLGVQPPILHMAN
                     SAGILFHPTAHLGMVRPGLMLYGYAPRQESPPQSLTLEPVMQATTYLAHLRSLQPGEI
                     VGYNALFRTRRPSQIAVLPVGYTHGFPRHLTGVGHVLIKGEQAPIIGKICMDMMMVDV
                     TDIPHPTVGDEVVLLGKQGTREITAEDHAGWLNTIPYEVLCGIGGKANRIYLPPILDP
                     SPSHTPTQSAKKILE"
     gene            complement(1274..1831)
                     /gene="frr"
                     /locus_tag="VLA60_07625"
     CDS             complement(1274..1831)
                     /gene="frr"
                     /locus_tag="VLA60_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HSF09266.1"
                     /translation="MNDPTILKTTQRMEGAIEHLKREFMSLRTGRASLGLLDHITVDY
                     YGTPTPLKQVANLAIPESRLITIQPWDTSQIREIERAIISSELGLTPSNDGKLIRLPI
                     PPLSEERRKDLVKLSKKYGEETKVQIRGFRREGNDELKHLHKEGSLTEDTQHRLEAEI
                     QKLTDKFIQTVDDLTKKKEEEILAI"
     gene            complement(1846..2574)
                     /gene="pyrH"
                     /locus_tag="VLA60_07630"
     CDS             complement(1846..2574)
                     /gene="pyrH"
                     /locus_tag="VLA60_07630"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HSF09267.1"
                     /translation="MPLKYRRVLLKISGEMLAGEQTYGIQPSILHNLAAEIADVLTLK
                     VEIAIVIGGGNIFRGLAASASGMERASADYMGMLATLLNALALQNALESKDIPTRVLS
                     AIEMRQLAESYIRRRAIRHLEKKRVVIFAAGTGNPYFTTDTAAALRAMEIGADVIMKG
                     TKVDGIYDADPMTTPSAKRFTELPFLSVLNKSLKVMDATAITLCMDNDLPIIVFDLTS
                     SGNIRKILEGEPVGTIVSKNALVT"
     gene            complement(2574..3170)
                     /gene="tsf"
                     /locus_tag="VLA60_07635"
     CDS             complement(2574..3170)
                     /gene="tsf"
                     /locus_tag="VLA60_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HSF09268.1"
                     /translation="MSSLSALVKELRGKTGAGILDCQKALQDTGNDVEKAIDLLRQKG
                     LAAAQKKAGRETKEGIISSYIHSGSKIGVLIEVNCETDFVARNEEFQAFVKEVALQIA
                     ASHPLYIKREDIPVDLIERERNIYLAQMKESGKPEGTWEKIIKGKLEKYYQEQCLLEQ
                     AFIKDPSISIQDLLSQKIAKLGENLTISRFTRYQLGQD"
     gene            complement(3232..4014)
                     /gene="rpsB"
                     /locus_tag="VLA60_07640"
     CDS             complement(3232..4014)
                     /gene="rpsB"
                     /locus_tag="VLA60_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HSF09269.1"
                     /translation="MVTIKSLLEAGVHFGHQTNRWNPKMKRYIFGEKNGIYIIDLQIS
                     LQCFQKAYEFTRDTVAAGESVLFVGTKRQAMGIVEEEANRCGMFFVNQRWLGGMLTNF
                     QTLRRSINQLKKLEAAETDGTYERLKKKEIVRMKKEQAKLEKYLSGIKGMNDIPGCIY
                     ILDTRIQHITVKEANRLGIPVIALVDTNCDPEGVDFPIPGNDDAIRSLKLFTGQIADA
                     CIEGTELRRKRQESVANGEGFLPDNTEQGHPSVVPNKNTQPV"
     gene            4428..5783
                     /locus_tag="VLA60_07645"
     CDS             4428..5783
                     /locus_tag="VLA60_07645"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSF09270.1"
                     /translation="MVEVPLKLYVQALLKGAKEAARPMACLSSCVKNQALQAMAESLV
                     DHTQEILEANAKDLAGISKETDQQTHREASDRIRISEDTIRLMHQGLLDLQALPDPIG
                     EASHVWLTPDGMQVHTVRSPLGIVAVVSDMGPLVTAESLGMCMKTNNVCVFRGGTEWF
                     HTNSAIVHYLQAAAAANGVPKGGLTFLDRSSPEAALEMVRYPQYVQAVITRGSAGLRN
                     GILEHSRVPVIGFEGGLCHVYVDQDVDIPLAQTIAVNAKLQSPSAANAMDTLLVHQGV
                     SRHLLPGLTRRLLQEYRVALRGCPKTVSMMGILEMTGHLGIKAAEEADWGRKYQSLTL
                     NIKVVKDSQEAIDHIGAYAPGHTDTIVTRDYQLAMRFVREVDSSAVMVNASTRLHGGP
                     QFGLGPDIGSNSTRIHGRGPLILSKLTIEKYMVLGTGQLQQPHPVPQTYQDAMMLSAK
                     F"
     gene            6006..>6311
                     /locus_tag="VLA60_07650"
     CDS             6006..>6311
                     /locus_tag="VLA60_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09271.1"
                     /translation="MLSWGLRDFHDEASYYDWQRETLSKGELCDFQRLIERRCGGEDV
                     KGDIDFYDELARRDLIPVLYSQRFHYFLTIGTSVCARITPANQVLDFGCGVGILTLFF
                     "
BASE COUNT         1482 a         1375 c         1624 g         1830 t
ORIGIN      
        1 caggccggcc gtgttgtgaa ggcatcggtg tgttgaggag ttattccaat atttttttgg
       61 ctgactgggt tggggtgtgt gaaggagagg ggtcaagtat ggggggaagg taaatccggt
      121 tcgcttttcc tccgatcccg cacaacactt catagggaat cgtattcaac catccggcat
      181 gatcttcagc tgttatttcc cgtgttccct gtttccccag taacacgacc tcgtctccaa
      241 ccgttggatg gggaatgtcc gtaacatcta ccatcatcat atccatgcaa atttttccaa
      301 tgatgggggc ttgttctccc ttgatgagga catgtccgac tcccgtgagg tgtctgggaa
      361 atccatgggt ataccctacc ggcaggacgg cgatttgaga tggtctccgt gtgcggaata
      421 gggcgttata tcccacaatt tccccaggct gtagagaccg gaggtgggcc agatatgtgg
      481 tggcttgcat taccggttca agagtgaggg attggggagg actctcctgc cggggtgcgt
      541 atccatataa cattaatcca ggcctgacca ttcccaagtg ggcggttgga tgaaacagga
      601 ttcctgcgct attggccata tgaagaatag gtggttggac tcccaattgc tttgcctggt
      661 cgataaaggc caggaatttt tggatttgaa cattggttaa ggtgtgatcc tgattgtcgg
      721 catcagcaaa atggcttaac aggccctcta tttcaatgag aggggtcgag agcgatagcc
      781 tgcttagaag ggagagggct tcctcattct cgaagccgag tcggtgtaat ccggtgtcga
      841 ttttgagatg aatgggaaat ggggtggcgc gaggaaaaag taactcgata agttgttgaa
      901 cgatttctgc acgagaaatc accggagtaa gctgatggcg aataatttcg cttagatgtt
      961 gagggaggat gggccccatg atcaggatgg ggcagtgaat gtggtgctct cgaagagtta
     1021 tcccttccat gacggatgcc acaccaaatc caaaaattcc caggtgttcc agggtttgag
     1081 ccacaggaat cgccccatgg ccataggcgt cagctttaat gatggccaga atttttgtag
     1141 acggagcgac ccattgccgg atttgttgga aattcctggt tagggcatca agactgatcg
     1201 aaacggtaat cggagacgag acgtgtcgga ggtcggaagg tgaaggtggc ctgatcggta
     1261 tcacaaatat tccttaaatc gcgagtattt cctcctcctt tttcttggtg aggtcatcga
     1321 cggtctgaat aaatttatct gtgagctttt ggatttcagc ctctagccgg tgctgggtat
     1381 cttctgtcag tgagccttct ttgtgaaggt gtttgagttc gtcattgcct tcccgacgaa
     1441 atccacgaat ctgtaccttg gtctcttccc cgtatttttt gctgagtttg accaaatctt
     1501 tccgtcgttc ttcactcagt gggggaatgg ggagccggat gagtttgcca tcatttgatg
     1561 gagtgaggcc aagttctgaa ctaatgatgg cacgttcaat ttctctgatt tgggaggtat
     1621 cccagggctg aatggtgatc agtcggcttt caggaatcgc gaggttggcg acttgcttga
     1681 gtggagtggg cgtcccgtaa taatcaacgg tgatatggtc gagtaatccc aaggaggcgc
     1741 gaccggttcg aagggacatg aattctctct tgaggtgctc aatggctccc tccattcgct
     1801 gtgtggtttt aagaatggtt ggatcgttca tgatcattcc ccacattaag tgacaagcgc
     1861 atttttggaa acaatagtgc cgacaggttc cccttcaagg atttttctga tatttcctga
     1921 tgaggtcaga tcgaatacaa tgataggcag atcgttatcc atacagagtg taatggctgt
     1981 ggcatccatg actttcaagc ttttattgag caccgataaa aatggtaatt ccgtgaagcg
     2041 tttggccgat ggtgtcgtca tagggtccgc gtcgtagatg ccatcaacct tcgtgccttt
     2101 catgataaca tccgccccaa tttccatggc tcggagcgcg gcagcagtgt cggtggtaaa
     2161 gtagggattc ccggtaccgg cggcaaaaat cactacgcgc tttttttcca aatgccgaat
     2221 agctcgacga cgaatgtaac tttcggctaa ctgccgcatt tcaattgccg ataagacacg
     2281 ggtggggatg tctttgctct ctaaagcatt ttgtaaagcc agagcattca ataaggtggc
     2341 gagcatgccc atgtaatcgg ccgaggctcg ttccatgcca gaggcactgg cagcaagacc
     2401 tctgaaaatg ttcccacctc caatcacaat ggcgatttcg accttcaggg tcagtacgtc
     2461 ggcaatttcg gcggcgagat tatgtaaaat ggaaggttgg atgccgtaag tttgctctcc
     2521 ggcgagcatt tctccactaa ttttcaagag gacccgacga tatttgaggg gcattagtcc
     2581 tggccgagct ggtagcgcgt aaagcgactg atcgtgaggt tttcccctaa cttcgcgatt
     2641 ttctgcgaaa ggagatcttg aatgctgatg ctgggatcct taataaaggc ttgttccaag
     2701 aggcactgct cttgataata tttttcgagt tttcccttga tgattttttc ccaggtccct
     2761 tcgggttttc cggactcttt catctgtgcg agataaatat tcctttcccg ttcgatcaag
     2821 tccacaggaa tgtcttcacg tttaatatac agaggatggg aggcagcgat ttgtaaggcc
     2881 acttccttaa cgaaagcttg aaattcttca ttgcgtgcga caaagtctgt ttcgcaattg
     2941 acctcaatca ggactccaat ctttgatcct gaatgtatat acgaggaaat gataccctct
     3001 ttggtttctc ggcctgcttt cttttgtgca gcggccaacc ctttttgacg caacagatcg
     3061 atggcttttt cgacatcatt tcctgtgtct tgaagggctt tttggcaatc aaggattcct
     3121 gcccctgttt tgcctcgcaa ctccttcacc aaggcactca aactagacat gtgcatgctc
     3181 tcctactgac agtaatatta atttcaattt aagtagatga acccaggatc cttagacagg
     3241 ttgggtattc ttgtttggca caaccgatgg atgaccttgt tcggtgttgt cgggaagaaa
     3301 cccctcgcca ttcgccactg actcctggcg cttccggcga agttctgtac cttctatgca
     3361 ggcatcggca atttgtccgg taaacaattt gagggatcgg atggcatcgt cattaccggg
     3421 tataggaaag tccacgcctt cagggtcgca gttggtgtcg actaaagcaa taaccggaat
     3481 gccaaggcga ttagcttcct tgaccgtaat gtgctggatc ctggtgtcca atatgtaaat
     3541 gcatccggga atatcgttca tgcctttgat gccgctcaaa tatttttcca gtttggcttg
     3601 ctcttttttc atgcgaacga tctctttctt tttgagtcgt tcgtatgtgc catcggtttc
     3661 cgccgcttca agttttttga gttggttgat gctgcgacgg agtgtctgaa aattcgttaa
     3721 catgccaccc aaccatcgct ggttaacaaa aaacattcca caacgattgg cttcttcctc
     3781 cacaataccc atggcttgcc gcttggtccc cacaaataag acggattccc cggcagccac
     3841 tgtatcacgg gtaaattcgt aggctttttg aaagcattgg agagaaattt ggagatcaat
     3901 aatataaatg ccgttttttt ccccaaagat atagcgtttc atcttcgggt tccagcgatt
     3961 tgtttgatgc ccgaagtgaa ccccagcttc taataacgat ttaatcgtga ccataacgga
     4021 ccttcccacc ccctttcaga gatatacgga ttaacacaaa accgtggatt ttttaaagaa
     4081 acacgaaaca gtaattaggc tcaaatggga ccggcaggag acaatagata tggattgtct
     4141 tatcaggcag gctgttccaa gtgaagagac attcatgcaa ttaagtgatg ttcaatattt
     4201 ttccattaaa ccaatcgaga ttagcatagg tgaaaattat aattcaaccc cgactatctt
     4261 ttatgggtgc gcaacgcgcc cttagctttg gagaggtatc gtagaataaa ctgaaagtat
     4321 tctgatgaaa atcttttttg aatcaatgct ttttttgact ctgtgagcct tcatggctat
     4381 agtcagtccc gcattcagaa attttttatg atgaaggagt gcgaaggatg gttgaagtac
     4441 ctttgaaatt atatgtgcaa gcgcttctga aaggagcgaa ggaggctgct cgccccatgg
     4501 cgtgcctgtc ttcctgtgtg aaaaatcagg ccttacaggc gatggccgag agtttagttg
     4561 atcatactca ggaaatttta gaggccaatg caaaagatct ggctggcatt tctaaggaaa
     4621 ccgatcaaca aacccatcgt gaggcgtcag atcggattcg aatttcagaa gacacgattc
     4681 gcctcatgca ccaagggttg ttagatttgc aagctcttcc tgatccaatc ggagaggcga
     4741 gtcatgtctg gctgactcca gatggaatgc aggttcatac tgtccgttct cccctgggta
     4801 ttgtcgcggt ggtgtcggat atggggcctt tggtgaccgc tgaatcgtta gggatgtgca
     4861 tgaagaccaa taatgtctgt gtctttcgtg ggggaaccga gtggtttcat actaatagtg
     4921 cgattgttca ctatctccaa gcggctgcgg cggccaatgg tgttcctaaa ggggggctga
     4981 catttctcga ccggagttcc ccggaggcgg ccttggaaat ggtgcgatat cctcagtacg
     5041 tccaagccgt gattacacga gggagtgctg gtttgcgaaa tgggatcttg gagcattcga
     5101 gagttcctgt cattggattt gaggggggtc tgtgtcatgt ttatgtcgat caggatgtcg
     5161 atatcccttt agctcaaacc attgcggtga atgccaaact tcaatcgcca tctgccgcga
     5221 atgccatgga tactctcttg gttcatcagg gcgtttcccg gcatttactg ccagggttaa
     5281 cacgaagact tcttcaggag tatcgggtgg ccttgcgtgg ctgtcctaaa acggtttcca
     5341 tgatgggtat tttggaaatg accggacatt tagggattaa agcggcggag gaagcggatt
     5401 gggggagaaa atatcaatcc ttaactctga atattaaagt agtgaaggat agtcaagaag
     5461 ctattgatca tattggggcc tatgccccag ggcataccga tactattgtc actcgggatt
     5521 atcagttagc catgcgcttt gttcgagaag tcgattcgag cgccgttatg gtgaatgctt
     5581 cgacccgtct tcatggcgga cctcagtttg gtcttggtcc ggatatcggg agcaatagca
     5641 ctcgcattca tgggcgaggt cctcttattc tctcgaagct cacgattgaa aaatatatgg
     5701 tattgggaac cggacaactt caacagccac atccagttcc tcagacctac caggatgcga
     5761 tgatgttatc tgccaagttc tagcttttga gatgaagcat gcatggccgg gccctttgtg
     5821 tcggctgtgc atatacccag gtctccgtta cccactcctg ttatcggtct ttcttctatc
     5881 cctgtgtttc ttttgtttcc catcatttcc tgtattcatt ttttagagga attatcttaa
     5941 tctggtttat tatggggtat aaaagggaag aaatttctga ttaccatgcg gagcttttcc
     6001 cgtatttgct gtcatgggga ttgcgagatt ttcatgatga ggcgtcatac tatgactggc
     6061 aacgagagac tctttccaaa ggggaattgt gcgattttca acgattaatc gagcgtcgat
     6121 gcgggggtga agacgtaaag ggcgacattg atttttatga tgaattggcg cgtcgggatc
     6181 ttatccctgt tctgtattct caacggtttc attatttttt gacgattgga acctctgtgt
     6241 gtgccaggat tactccagcc aaccaggtat tggactttgg ttgtggggtt ggcattttga
     6301 cactgttttt t
//
LOCUS       DATIAL010000247        13790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1443772, whole genome shotgun sequence.
ACCESSION   DATIAL010000247 DATIAL010000000
VERSION     DATIAL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13790
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443772"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..1788
                     /locus_tag="VLA60_07655"
     CDS             328..1788
                     /locus_tag="VLA60_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HSF09272.1"
                     /translation="MESDNTIELLPDTRSDLQEEIQFSRWLTGLCLLFCAGGLGLLFW
                     AVTLSDDVRLASPASRDLERIASRMLGFESRLPDLSSFEQVMYRLGGQDGETLEQIQL
                     WYEERVDDQSSPLDRLYLGMLYGEAGLTDQFKQFVMSWSMVHNPPALFRRLLEVGYGQ
                     SETSPADYVFLQARLAEEVPANWFYFHLARRIAVQAGDRDLQENLQLQEYQLTDPPLW
                     KWRVLLVGEVVVIGIGVVVFLRLGFARVKGRISQSQTDLAAWRIPWTFREGIAVLARG
                     GALTILLMALVAVMPDGIGIIEDFGIALLYLPPVLLTSLLLCRPRKQSLLQAVGCSNV
                     WPRLKSGLPLVVMLVTLGLVGDWLIVLGGDAFQSSVHWTEWFVPQLIWGTRMELIKTT
                     IDFVLLAPFFEELIFRGILYTTLRTKFSFPLSMVASGLIFALAHGYGLIAFLTVLWSG
                     LLWAWAYERTGSVIPGMVAHAVNNGVVVYSLVAFFR"
     gene            complement(1823..2620)
                     /locus_tag="VLA60_07660"
     CDS             complement(1823..2620)
                     /locus_tag="VLA60_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09273.1"
                     /translation="MSPSLLQHIPLVILGTGYTGRRLYHQAVDAGWNAFTTSRMPDVH
                     LSDISPDHHIHFDLTRPETWRSIPTPAHLIWCFPATPLNVVREFFAGRPRESGRILVM
                     GSTSAYGTNCGAVTEQMPVNMDLPRVQGEEYLRTQLGAVVIRLAGLYGPGRHVLDWMR
                     KGRTRNTPKWVNLLHVEDAAGICLRALERAQNGTTYLASDGTPRTWSEIFSIASEKWS
                     IPIPPPTPLREIGKQVSIHKLLTTLHYTLRFPDLYQALDDIERHSSS"
     gene            complement(2711..3229)
                     /locus_tag="VLA60_07665"
     CDS             complement(2711..3229)
                     /locus_tag="VLA60_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HSF09274.1"
                     /translation="MLRTYRGIAPTVATSAFIEDTAVVIGDVAIGSESSVWFHAVIRG
                     DVHSIRIGHRTNIQDLCLLHVTHDTHPLTLGDDITVGHHVVLHGCTIHDRVLIGMGAI
                     IMDGVVIEEDCVIGAGALITGNIHIPSRSLVVGSPAKVKRLLTDAEVQWIKESAANYI
                     RYAQQYVSEQNE"
     gene            complement(3285..3761)
                     /locus_tag="VLA60_07670"
     CDS             complement(3285..3761)
                     /locus_tag="VLA60_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09275.1"
                     /translation="MPDSPHTNRELSQAQLETIRMLYPLFKNEVLRRREYMSRLSVFH
                     NTILVFLIMIIAVVSPGHPDSTMRWLTISGVVILSGFVVYLILQQATRHRMAKQQLIE
                     LERGMGLYQEDWMFSGKTTYPQHWQTDWLSDRSVATYLTILIVLTGLAIYAMLIRL"
     gene            complement(3780..4634)
                     /locus_tag="VLA60_07675"
     CDS             complement(3780..4634)
                     /locus_tag="VLA60_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF09276.1"
                     /translation="MARPTISTFCKHSQLVETASPTESWDGRTEAPVSFTGSSGIVTT
                     GIFAQPAASTNRAVILCHGFPSDKNSRTNRRLTDLLIPQSIATLRFDWYGMGDSPEPL
                     SQLRIKKCEEQLDAAVRFLTERSMQALGLIGSSFGGFMAILSAPRYPHLQALGLKCPV
                     VDFSEVLRLELGADAMDQWKRTDHIPDVFGGDLPTPLPYAFFEECLTYNGYNSASRIQ
                     APTLIVHGDQDEIIPVHQIDRLLTSLSVPKDFRLIPGANHQFGRPEDFRLMTAHLANW
                     MIAHLFIS"
     gene            complement(4597..5388)
                     /gene="thiD"
                     /locus_tag="VLA60_07680"
     CDS             complement(4597..5388)
                     /gene="thiD"
                     /locus_tag="VLA60_07680"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HSF09277.1"
                     /translation="MKKAVCTIAGSDCSGGAGIQADLKAMSANGVFGMSVVTSITAQN
                     TCGVSDVFHLPISIITAQLDALFSDIPVTVIKTGMLATSDIITTVSRHLAKQHLEHLV
                     VDPVMMAKGGYPLLEQEAMATLKTELLPQASLLTPNIAEAEMLSGLSIKTLAHAREAA
                     KVIHQFGCQHVLIKGGHLLEQPGTDLLYDGRFFRMFKEEFIQTNTTHGTGCTLASAIA
                     ANLAKGKPIPDAIEAAKHYTTEAIRHGLPFGQGNGPTDHFYFLQA"
     gene            5636..9052
                     /locus_tag="VLA60_07685"
     CDS             5636..9052
                     /locus_tag="VLA60_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF09278.1"
                     /translation="MESSHPSKSSPPSDEEIHRKRLGDLTLSLGSSQGSPAQLSSKSE
                     ASSHNIRLPETRQSKISPGTPQPELQEELYALRQQVERFILASQGSREGLWVAEIRPD
                     VPWYSPECPVWYSPQFIAMLGYEESEFPGVLGSWEQRLHPDDRERVFEALTNHIEKRI
                     PYHVESRLLTKSKGYRWFEATGEGTFDADGRLLRGGGTIRDITERKLAEDISRRNHAL
                     LDAVLEEMSDVVYVKDQDGRYLLVNPSGAEIMGKAIHEVIGKTDQEIFGTQPHALFVE
                     GDDHVMQSAGTQTFEVEVQEQHPVSRTFLVTKDSFGSLPDGQNGVLGFARDITFRKAA
                     ELIIQEREKRFRAIMENAYDLITETDAEGRFLYVSPNFKESLGYSSQDLLGTSVFAPV
                     HPEDRDRVVEEFCQGMKTHGSGRSVYRYRHQNGEYHWFESTGRAFQTALGELRAVVIS
                     RDITQRKQWEDALEAIVKGNVIPGSPNFFEVLVGELAKALQVPMVFLSERIEPNASKA
                     RTLAFWNHDHFEPSSEYDCLGGPCEQVFQGNPVDQPSGVQHLFPRSETIRALGVEAYY
                     GIPLFNAQNEVVGNLALLDTNPLVLSSQGHNLLKIFAARAGAELERKRAQEEVESSQD
                     KYRELYDQTPLIYFTVNSQGIILSVNQYGAQVLGYRVEELLGTSAFSVVYEEDRALFQ
                     SGLEKGLRESIKGIIPEFRKVKKDGTIIWVKETIQAIEKQSGPRVWLLSCEDITERKR
                     TEEALLLSEMQLRHTQKMEAIGTLAGGIAHDFNNILGAILGYSELAMAYATQDERLKS
                     YLNEVVAAGNRARDLVKQILAFSRRSEKDKEAIDLRLVIRDVLKMVRASFPSSIEIRT
                     SLDLESTVIYGDRTQMQQVIMNLCANAEYAMREEGGLLEIALGHECIPEDGMPGLGLC
                     KAGSYLQVVIRDTGNGIPLEMVERIFEPFFTTKPAGEGTGLGLAVVHGIVHNHGGGIA
                     VSSRPGEGTTFTVLLPRLDVIAPEKPEEVIAWPTGSGRVLFVDDEEMLTRWGTQFLSH
                     LGYAVVASVNPYEALDLFRAHPSDFDVVVTDQTMPTMSGEALSRALLGIRADIPILLC
                     TGFSHTMTQEKAKQLGIRAFLMKPVNGLSLALALRNILGGGPTPDPTLHS"
     gene            10168..10449
                     /locus_tag="VLA60_07690"
     CDS             10168..10449
                     /locus_tag="VLA60_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09279.1"
                     /translation="MEVEVSGEVYSGASVVAMSVFRHPDIPLGHGKLDLDMEREAVVV
                     ELPDNRQLFALLTYNAYLAVKAFSYVMRAGKTANRPTSGHRDCPEGQSP"
     gene            10421..10741
                     /locus_tag="VLA60_07695"
     CDS             10421..10741
                     /locus_tag="VLA60_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09280.1"
                     /translation="MTAQKGKVREVAPKDNPLLVTFTNITNPTTVQRVDPDNLAATCG
                     PGVSLKRITLEITDELVTEGKIESVLGWLKTIGGGMLDGRCISTINAENRLANDLTRV
                     DFIR"
     gene            complement(11135..11608)
                     /locus_tag="VLA60_07700"
     CDS             complement(11135..11608)
                     /locus_tag="VLA60_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09281.1"
                     /translation="MKILGVYQNSSKSGPPKVPIKDRILLVDDDRHHRTCLKEFLELR
                     GYLCSEAGNGVEGLEILQKESVSIIITDNNMPQMDGLDFIEQVNHKYSQEILSIFLIT
                     AELSHIVRLRAFKNGVNRVFEKPLDFQELCNAVDWVTKFDIHQSTTNKYSSARYL"
     gene            12995..>13790
                     /locus_tag="VLA60_07705"
     CDS             12995..>13790
                     /locus_tag="VLA60_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF09282.1"
                     /translation="MIEVLPFSNVRRVGKFGLLGGCLIVVMAGCSSKLQTTVISVTEP
                     PAKPQVVKVEPVTPPVEAVVQPPVQAETFIGISPMDIPVEEPARAVVRPSAPADIFAT
                     PRTTVVEPAVSAPLEAPLVAEPVIPPSPIIPTTPDQVSPQESLQDLGIPPIAFEPEMP
                     AQPVIRGDAAPPEQMIARMEPEADKSAQPTPALPEMIEKKTEALLKSLGDIYFDYDRF
                     SIRADAISVLQENAQALASGLANNKIVIEGHCDQRGTESYNMVLGER"
BASE COUNT         3316 a         3056 c         3878 g         3540 t
ORIGIN      
        1 gtggtccatt catcgctatc agacgacttt taacccggac cggctcacaa aaggcttgac
       61 ctaaaccata gaatctcaga caaggccgcc ttggctctgg attcggttcc gcgtctcggc
      121 cacaccgaat gggcgatata attgttaatt aaaaaaagag taggtcagtg agatggctag
      181 ttgcctttgt cattatccct tacaggaggt gtggagtgta tccgtggcgg gtacggtatg
      241 gtttgtttcg gcagggcgcc tgcagatcat ggtatcttct ctggtagtta aaacaagtaa
      301 ctttcttgca tctgtgaacc ctctcttatg gaatcagata atacaatcga gctcctccca
      361 gacacccgct cggaccttca agaagaaatt caattctccc gttggctgac cggcctgtgt
      421 ctgttgttct gcgcaggtgg gctgggattg ctcttttggg cggtgacgct ttctgatgat
      481 gtgcgcctgg catcacctgc aagccgggat cttgagcgga tcgccagccg gatgttgggt
      541 tttgaatctc gccttcctga cctctcttcg tttgaacaag tgatgtatcg tttgggggga
      601 caggacgggg agacgctgga gcaaatccaa ctctggtatg aagaacgggt ggatgatcaa
      661 tcctcaccat tggatagatt gtatttgggc atgctctatg gcgaagccgg gttgaccgac
      721 caattcaagc agtttgtgat gagttggagt atggtgcaca atccgcctgc tctttttcgt
      781 cgattgttgg aagtgggata tgggcagagc gaaacgtcgc ctgctgatta tgtcttcttg
      841 caggcgaggt tagctgaaga ggtccccgca aattggtttt actttcatct tgcgcgacga
      901 atagccgttc aggccggcga tcgggattta caggaaaatc tgcaattaca agaatatcag
      961 ttgaccgacc caccattatg gaaatggcgt gtgctattgg tgggtgaggt tgtggtgatt
     1021 gggattgggg tggtggtctt tctccgtctg ggtttcgccc gggtgaaagg ccggatttca
     1081 cagtcccaaa ccgacttggc tgcctggcgc attccatgga cattccggga gggtatcgcg
     1141 gtcttagccc gtggaggggc cctgacgatt ctgttgatgg cgctggtggc agtcatgcct
     1201 gatggtatag gaattattga ggatttcggg attgctttac tgtatcttcc tcccgtcctc
     1261 ctgacctccc tgctgttgtg tcgacccagg aaacaatcgt tgctgcaggc cgtgggctgt
     1321 tcgaatgtgt ggccacgatt gaaatccggt ctgccattgg tggtgatgtt ggtgacgctt
     1381 ggacttgttg gggactggtt gattgtgctc gggggggatg cgtttcaatc ttcggtgcat
     1441 tggacggaat ggtttgtccc gcagctgatt tggggaacgc ggatggaact catcaaaacc
     1501 acaatcgact tcgttcttct ggcgcccttt ttcgaagaac tcattttccg gggaattctc
     1561 tatacgacac ttcgaaccaa attcagtttt ccgctctcca tggtcgcaag tggattgatt
     1621 ttcgccttag cccatggcta tgggctgatt gcatttctga cggttttgtg gagtgggttg
     1681 ttgtgggcat gggcctacga acgaaccggg agtgtgattc ccggcatggt ggcgcatgcc
     1741 gtcaataacg gggtggtggt gtattctctt gtcgcatttt tccgttaaga atgatcatgt
     1801 ggctgcttgg ggtgctgtgt tttcatgatg atgaatgccg ttcaatgtcg tccaaggcct
     1861 gatagaggtc aggaaatcga agggtatagt gaagggtggt gaggagtttg tgaatagaga
     1921 cctgtttgcc gatttcccga agaggcgtgg gtgggggaat gggaatgctc cacttttctg
     1981 acgcaattga gaaaatctct gaccaggtcc gtggggtccc atcgctggcc aaatacgtgg
     2041 taccgttctg agcccgttca agggctcgca aacagatgcc tgccgcatct tctacatgga
     2101 ggagattgac ccatttcgga gtattgcgag tcctgccttt tctcatccaa tccaacacat
     2161 gacgacctgg cccgtataat cctgccagtc gaatgaccac agcgcccaat tgtgtccgga
     2221 gatattcttc tccttgcacc cttggaaggt ccatgttgac cggcatctgt tccgtgacgg
     2281 ctccgcagtt cgtgccatag gcggatgtgc ttcccatcac cagaattcta ccggattccc
     2341 tggggcggcc tgcaaagaat tcccggacca cattcaatgg cgtcgcagga aaacaccaaa
     2401 tgagatgggc cggagtggga atggatcgcc aggtttcagg tcgggtcagg tcaaaatgaa
     2461 tgtgatggtc aggtgaaata tctgagagat ggacatccgg cattcggctt gtcgtgaagg
     2521 cgttccatcc tgcatcaact gcctggtgat agagccgtcg accggtgtag ccggttccca
     2581 aaatgaccag tggaatgtgt tgaagaagag aagggctcaa gggagaggtc ttatccgaat
     2641 gtgcggttgg gaagaggaat ttttcaagag ccagggcata tcctgtttgg ggcttgatga
     2701 aattccgaca ttactcattc tgttctgaga catattgttg cgcgtatcgg atgtagttcg
     2761 cggcagattc tttgatccac tgcacttcag catccgtcaa caggcgtttc actttggccg
     2821 gagaccccac gaccaggctc cttgagggga tgtggatatt ccccgtaatt aaggctccag
     2881 cgccaatgac acagtcttct tcgatcacaa cgccgtccat gatgatagcg cccatgccaa
     2941 tcagcacccg atcatgtatc gtgcacccat ggaggaccac atgatggccc acggtgatgt
     3001 catccccaag agtgagaggg tgggtgtcat gcgtcacgtg caacaggcag aggtcctgaa
     3061 tattagtccg atggccaatg cggatagaat gcacgtcccc gcgtatgacg gcatgaaacc
     3121 agacgctcga ttccgatccg atggctacat ccccaatgac gacggccgtg tcttcgataa
     3181 acgcgctggt cgcgaccgtt ggggcaatcc ctcgatatgt tcttaacatg agcgcggtcc
     3241 tttcggcgaa tagaatatct ccatgtgagg acgtgaggtg aaggttagag gcgaatgagc
     3301 atagcgtaga tagccagtcc tgtgagaaca atgaggatgg tgaggtacgt cgccacgctc
     3361 cggtccgaga gccaatcggt ttgccaatgt tgcggatagg tggtttttcc cgaaaacatc
     3421 cagtcttctt ggtacagtcc cattcccctc tcgagttcaa ttaattgttg tttggccatt
     3481 cggtgtcgag tggcttgttg aagaatcaga tagaccacaa atcctgacaa tatgaccaca
     3541 ccggaaatcg ttaaccatcg catggtggag tcggggtgtc caggggacac caccgcgatg
     3601 atcatgatca agaacactaa gatggtattg tggaataccg acagccggct catgtattcc
     3661 cgccgccgga gcacctcgtt tttaaataac ggatagagca tacgaatggt ttcgagttgc
     3721 gcttgggata attcccggtt ggtgtgtggt gagtcaggca taaatcgggt ccggccacct
     3781 caagagataa atagatgagc gatcatccaa ttagccaaat gggcggtcat gagacgaaaa
     3841 tcttcaggcc gtccgaattg atggtttgca ccgggaatga gacgaaaatc cttgggcacg
     3901 ctcagagacg tcagtaatcg atcaatctga tggaccggaa tgatttcgtc ttgatcccca
     3961 tgcacaatca gtgtgggggc ttgaatgcgc gaagctgagt tgtagccgtt ataggtgaga
     4021 cattcctcga aaaaagcata cggcaagggt gtcggaaggt cgccaccgaa gacgtcgggt
     4081 atgtggtcgg tgcgtttcca ttggtccatg gcgtctgctc ccaattccag gcgaagcact
     4141 tcagaaaaat cgaccacggg gcatttcagg cccaaggcct ggaggtgagg ataccgggga
     4201 gccgaaagta tggccatgaa tccaccaaaa ctggatccaa tgagtccgag agcctgcatg
     4261 gatctttcgg tgagaaagcg gacggccgca tccagttgtt cctcacattt tttgatgcgg
     4321 agctgtgaca agggctccgg tgagtcaccc atgccatacc aatcgaatcg aagcgttgcg
     4381 atggattgag gtatgagaag gtcggtcagc cgccgattgg ttcgactgtt tttatcggaa
     4441 ggaaatccat gacaaaggat tacggctcga ttcgtggaag cagccggttg agcgaaaatc
     4501 cctgtcgtta cgatgccgga tgaaccggta aagctgacag gagcttcagt cctcccatcc
     4561 cagctttctg tgggcgatgc ggtctccacg agttggctat gcttgcagaa agtagaaatg
     4621 gtcggtcggg ccatttccct ggccaaaagg aaggccgtga cgaatggcct cagtcgtata
     4681 gtgtttcgcg gcctcaatcg catcggggat gggtttcccc ttggccaggt tggcggcaat
     4741 ggcagaggcc agggtacagc cggttccgtg agtcgtattc gtctgaataa attcctcctt
     4801 aaacatgcgg aaaaacctgc cgtcatagag taggtcggtt cccggctgtt ccagcaggtg
     4861 cccgcccttg atcaggacat gttgacagcc gaattgatga atgactttcg cagcctctct
     4921 ggcatgggcc aaagtcttaa tggagaggcc ggataacatt tcggcttcgg caatatttgg
     4981 ggtcagaagg gaagcctgag gaagcaattc ggttttcagt gtggccatcg cttcctgttc
     5041 caggagtgga tatcctcctt tcgccatcat aactggatca acaaccaggt gctcgagatg
     5101 ttgtttggcc agatgccgac tgacggttgt gatgatgtcg gaggtcgcca gcatcccggt
     5161 tttaatcacg gtgacaggaa tatcggaaaa gagcgcatcg agttgtgcgg taataatcga
     5221 gatgggcaaa tgaaaaacgt cagaaacgcc gcacgtattt tgtgcggtaa tggacgtcac
     5281 caccgacatc ccgaacaccc cgttcgcgga catggccttg agatcggctt gaattccggc
     5341 tcctccacta caatcagagc cggcgatggt gcaaacagct tttttcatgt gtgtcagtga
     5401 atgaagagtg tgtggacgaa cggagagtct agcatgtcat attctgatga tccagggatg
     5461 ccgcattcta gtgattggta agcgtggaaa tcctgctgtt ccagcttacg tctgacgatc
     5521 aggtgttttt gcgcattttt tcttgacatt ataggaaaaa tatttcatgg tgtttgatta
     5581 tagagtcact ccttgagaag ctggtcattc cgggcttttc aaggatagat gctgtatgga
     5641 atcttcccat ccttcaaagt cttctcctcc ttccgatgaa gaaattcatc gaaagcgcct
     5701 gggagatctg acgctttccc ttgggtcttc ccaagggtca cctgcacaat tatcttcgaa
     5761 atctgaagct tccagccata atatccgatt gcccgagact cgccaatcca agatatcccc
     5821 aggtacccca cagcctgagt tgcaagagga attgtatgct cttcgacagc aagtggagcg
     5881 gttcattttg gcctcccagg ggtctcgtga gggattgtgg gtggctgaaa ttcgtccgga
     5941 tgttccctgg tactctccag agtgtccggt gtggtactcc cctcagttta tcgcgatgtt
     6001 ggggtatgag gaatcagagt tcccgggagt actggggagt tgggagcaac ggttacaccc
     6061 tgacgatcgg gagcgagtct ttgaggcctt aaccaatcat attgaaaaga ggattcccta
     6121 tcatgtggag tcccggttac tcactaaatc aaaaggttat cgttggtttg aggccaccgg
     6181 ggagggaacc tttgatgccg atggccggtt acttagaggg ggaggaacca tacgcgacat
     6241 cacggagcgc aagttggcgg aggacatatc gaggcggaac cacgctctgt tagatgctgt
     6301 cctcgaagaa atgagtgatg tggtctatgt taaggaccag gatggtcgtt acctcctggt
     6361 gaatccttcg ggcgcggaga ttatgggaaa ggccattcat gaggtcatcg gaaaaaccga
     6421 tcaggaaata tttggcaccc agccgcatgc gttgtttgtc gagggggatg accatgtgat
     6481 gcaatctgcg gggactcaga catttgaggt cgaggttcag gagcagcatc ctgtgtcccg
     6541 aacgttttta gtgaccaaag actcgtttgg atctttaccg gatggtcaga acggggtctt
     6601 gggttttgct cgcgatatta cgttccgaaa agccgctgag ctcatcattc aggaacgaga
     6661 aaaacgcttc cgggccatta tggaaaatgc ctatgatctg attacggaga ccgatgcaga
     6721 aggacggttc ctctatgtga gtccaaattt caaagaatct ctcgggtaca gctcccaaga
     6781 tctgcttggc acgagtgtct ttgccccggt tcatcctgaa gatcgagaca gagtggtgga
     6841 ggagttttgt caaggcatga aaacccacgg ttccggccgt tcggtctacc gctatcggca
     6901 tcagaatggg gaataccact ggtttgaaag caccggacga gcctttcaaa cggcgcttgg
     6961 ggaactgcga gctgtcgtca tctcgcgaga tatcacacag agaaaacaat gggaagatgc
     7021 gctggaggcg attgtcaaag gcaatgtgat ccccggctct ccaaatttct tcgaagtcct
     7081 tgtcggtgaa ttggcgaaag cacttcaggt gcccatggtg tttttgtcag agcggattga
     7141 gccaaatgcc agcaaagccc ggacattggc tttttggaac cacgaccatt ttgagccttc
     7201 ctccgagtat gattgtcttg gtggcccttg cgaacaggtg tttcagggta accctgtgga
     7261 ccagccttcc ggtgtccaac acctctttcc ccggagtgaa acgattcgag ctctgggggt
     7321 tgaggcgtat tatgggattc ctctttttaa tgctcagaat gaggtcgtgg gaaatctagc
     7381 gcttttggat acgaatccgc ttgttctttc ctctcaaggg cataatcttt tgaagatttt
     7441 tgccgcacgg gcaggagcgg aacttgagcg caagcgggca caggaagaag tcgaaagcag
     7501 ccaggacaag tatcgtgaac tctacgatca gactccatta atttatttca cggtgaatag
     7561 tcagggcatc attctctccg tgaatcaata tggggcccaa gtgttggggt atcgggttga
     7621 ggaattgctg ggcacctctg cgttttccgt ggtctatgag gaggaccgtg ccctgtttca
     7681 atcaggtctg gagaaggggt tgcgtgaatc cattaaaggt attattcctg aattcagaaa
     7741 agtaaaaaag gacgggacca tcatttgggt gaaagagacg attcaggcca ttgagaaaca
     7801 gtcagggcct cgggtgtggt tactgtcttg tgaggatatt acagagcgga aacggaccga
     7861 agaggcttta ttattgagtg agatgcaatt gcggcatacc caaaaaatgg aggcaattgg
     7921 cacattggct gggggcattg cccatgactt taataatatt ttgggtgcga tcctgggata
     7981 ttccgaattg gcgatggcct atgccaccca ggatgaacgc ttaaagtcgt atttaaacga
     8041 agtggtcgcc gccgggaatc gagcccgtga tcttgtgaag caaattttag cctttagccg
     8101 gcgttcggaa aaggataaag aggccattga cctgcgattg gtcatccggg atgtcctgaa
     8161 aatggttcgt gccagctttc cttcctcgat tgaaattcga acgtctttgg atcttgagtc
     8221 cacggtgatt tatggtgacc gaacgcaaat gcaacaagtg attatgaatc tgtgtgccaa
     8281 tgcggaatat gccatgcggg aagagggcgg tctgttggaa atagccttag gccatgagtg
     8341 tattcctgag gatggaatgc cgggactggg cctatgcaaa gccggttcct atcttcaggt
     8401 ggtgattcgt gacaccggaa atggcattcc tctcgaaatg gtcgagagaa tttttgagcc
     8461 attttttacc acaaagcctg ccggagaggg cacgggactc ggactggcgg ttgtccacgg
     8521 gatcgtccac aaccacgggg gtgggattgc ggtgtccagt cgtccggggg agggtacgac
     8581 cttcacggta ctcctgccgc gcttggatgt gattgcgccc gaaaaacctg aggaagtgat
     8641 tgcctggccg accggatcgg gaagggtctt atttgtggat gatgaggaaa tgctgacgcg
     8701 ctgggggaca caatttctca gccatttagg ctatgccgtt gtggcaagcg tcaatccgta
     8761 tgaagcgtta gatctgtttc gggcacatcc ctcggatttt gatgtggtcg tgacggatca
     8821 aaccatgccg acgatgagcg gggaggcctt atctcgtgcg ttgttaggaa ttcgagcgga
     8881 tattcccatt ctgttgtgta cggggtttag ccacaccatg actcaagaaa aagccaagca
     8941 actcggcatt cgggccttct tgatgaagcc ggtcaacgga ctgtctctgg ctcttgcgct
     9001 ccggaacatc ctgggtggag gtcctactcc ggatcccacg cttcattcct gagggggcat
     9061 ctgacgttaa gccaccatta taggcgtgtt tccgctctct tgaattgttt gtgagagatc
     9121 gtccgacctt ttagccaggt gccaccgtag ccggttttcc tcatcaggtg cctttaaatc
     9181 ccttttagta ttggccatcg acaaaggtat gtttatggtc gcctttggtg cccttttgta
     9241 acgtctacgt tagttcaacg gttgatccga cgatttcgcc attgcgcggg gaggaaatcg
     9301 tccgctgaga tccttccttt tcctaggcag aagccgacaa cccacccata gcgataacgc
     9361 ttcccacgat tgccacttgg aatatccagg atttcatacg tctctccttg gtcttcgaag
     9421 gttttattga atgaattttt ctatgaggtt tacaaccgga gaattattgc cgaaaaaaat
     9481 tttggaaggc aaaactccat gcagcttgaa atacgaatgt cactaaggga tgccatcgtt
     9541 aatcaaccaa tgaattaagt caggtaggaa tctggaagag acaggctctc tgtagaaacc
     9601 gatgaaggtt agggagccca cgtaaacgga gctatcattg gcgagatttt agtcttggga
     9661 agtttttcgc ccatgtatat gtccaaaagt ctcagagata taattaagcc ggcgttcgtc
     9721 ccggcagccg agccactttt gtttgggcaa aagtagccaa aaccagggac gccccgtccg
     9781 gcttcatcgt atgcgacgga cgcgaactat ggaagagcgg gctaactcgc ggggctcaaa
     9841 caaggcccgc gaaaagatca gagcgtccgt cccgtgggcc ggacggcagg cgtcgaaagg
     9901 ccaaggagaa gggggctaag ggtgatcgag attttgactt gcgagttgag gaatgccttt
     9961 gagccacatt cgtggagtga aggctgtaca acgtgatccc agaaaggcac gcgtagacat
    10021 gaccaggaag ggaacttgtt ggcagatgta caaaaaagga ctcagtctaa aagaattggt
    10081 gctcatactt tgtctatttc ttatcccact cctgaccgga tttgagttgt tgggtttgct
    10141 tcgtactcgt ggcatcagaa gatgacgatg gaagtggagg tgagcgggga agtctactcg
    10201 ggggccagtg tggtcgccat gagtgtcttc cgacatcccg atatcccgct agggcatggg
    10261 aagttggatc tggatatgga gagagaagcg gtcgtggttg aactccctga taatcggcag
    10321 ctgtttgcgc tgcttaccta caatgcctat ttagcggtaa aggcgttttc gtatgtaatg
    10381 agagccggaa agaccgccaa tcgtccgacc agtggccatc gtgactgccc agaagggcaa
    10441 agtccgtgag gtggccccca aggacaatcc gctgttggtg accttcacaa atatcaccaa
    10501 tcctacaacc gttcaacgag tggatccgga caacttggcg gccacgtgcg ggccgggggt
    10561 ctccttgaaa cgcatcacgc tggaaatcac cgacgagctg gtaacggagg ggaagattga
    10621 aagtgtgttg gggtggttaa agactattgg tggtggaatg cttgatggtc gatgcatatc
    10681 aacgataaat gcagaaaata gactggcaaa tgatttaaca agggtggatt ttattaggta
    10741 aggagattag gacatggccg tttcttctga acttatgtat cgccattctg gcgatgtatt
    10801 cttactaccg agggtataac tccggcatcg aaggattagg gggaataggc tctagtagtg
    10861 gagatggcat taggaacgcc actgtggttt ccgaggatcg aagctcggac gctcaggaca
    10921 ccggcttcta cgcggtggcg tatgagtgga atggagagac cattatttct tgccgaggaa
    10981 cagatagcaa gggagaatta cttggtacgg attatcccat tgtggattag gataatgatg
    11041 attatgaaga agttcgggta tttccttcga ggaggatccc cgatgctgag acttcatcag
    11101 gccgaaaatt tgtcttttgg aagagagctg aagcttaaag gtatctggcg gaggagtatt
    11161 tgttggtcgt tgattgatgg atgtcaaatt tcgtgaccca gtctaccgca ttacagagtt
    11221 cttgaaaatc caggggcttt tcaaagaccc gattgactcc gtttttaaat gcgcgaagac
    11281 ggacgatgtg ggataattcg gcagtgatga gaaaaatgga gagaatttcc tgtgaatatt
    11341 tatgattgac ttgttcaatg aaatcaagtc catccatctg tggcatattg ttgtcggtaa
    11401 tgatgatgga gacggactct ttttgcaaaa tttctagccc ttccacacca tttcctgctt
    11461 ctgaacacaa atacccccga agttctaaaa attctttcag gcaggttcga tgatgtcgat
    11521 catcgtcaac taacaggatt ctgtctttga tgggaacctt gggagggccg gattttgatg
    11581 aattttgata cacgcctaaa attttcatgg tgctttacaa ttattggtgt cgaaataatt
    11641 tctctcaaaa ggtggcggag ggacttttga gaaggtggct gatgaggaga ccagacttcg
    11701 ggtgcggtcg aaatcgtagt tcccctgaaa acagtccctc cgccgaggct cataaaattg
    11761 cgctcaattc cagtatggcc atgtttcaac ttttattagg acatccaaac cacgtcgggc
    11821 tccatatcgt tgaactagtt aatgaaacgg catattcaaa aagttacttg gcctcaatcc
    11881 caataattgg tgagttgcaa tagtatagaa gatttaaatg caaggggtgt tccagatgat
    11941 tttttttata aaagtggagt aatttcattt agttatggat tgcctttgga taaattggat
    12001 aaaaatcatg caggtgaaac cctacaggac gcagtcgact tccttcagaa gtaacggggg
    12061 cgtaaggata aggaccagtc atggacttta tgatcgttta attcatccat agcctcactg
    12121 aagggaagca tttgaggttg cttgagcttg ggaagagctc gctacggaac gccaggaata
    12181 gaggggttct ggcattccta tattgaaatt agaaagtatg agaaattttc aaaagtcccg
    12241 gaaatggatg aatgagtcac gataagaggt gattcgtgag catgaaacgg aaagtgccgg
    12301 ttcgtacatt aggattgatg gggctcaaaa taatttcaaa ttggggctga cggtctaggc
    12361 aacttcatta tgaactgggg cgtattgatt tgggtactgt ttcgacgttg cactgaggat
    12421 tcgatagagg ctcgcccttc ggagaatcat cttttgtggc ggactgggag aactttaaag
    12481 aacccaaaaa aactctcggg tcttctggtc tcatgttgcc aaatagttgg tgaatgccat
    12541 tctctgaaaa atcctgacgg aggggattcg gagttttcga tagatatttt ggataaacaa
    12601 aaaacagttt tcatagttgg ggaagacacc aggctatcgt gatgcaacat aaagggatgt
    12661 tcgacagggc aaggcttgct ccattggacg tgcaaagatc aggatgaaag gttgtttcac
    12721 tcagggattt catgtagttt tgttcgtagg acccttgacc tggatcttac caaaaggcat
    12781 tcttaagtga ggagactgga gtaggttaca ggaatgccac attcgagttc ttgacacaat
    12841 tctaagggac tgcatagagt tttgaaatta gattatcatg aggaaagaca gagtccttgc
    12901 ttagagtaat tcttgaaggg catgaggaat caaattgaat gggatgtcgc cgaggcatcc
    12961 gtcagtcaag aaagggaaat ggtggaggta aaacatgatc gaagtattgc ctttttcgaa
    13021 tgtgagaaga gtgggaaaat ttggcctatt gggcggatgc ctgattgtgg taatggcggg
    13081 gtgtagttcc aaactacaaa cgacggttat cagtgtgact gaacctcctg ccaagcccca
    13141 agtggtaaag gttgaacctg tgacgccgcc tgttgaggcg gtcgttcagc cgcccgtgca
    13201 agctgaaacg ttcattggga tttcccctat ggacattcct gttgaagagc cggcccgcgc
    13261 ggttgttcgt ccttcggctc ctgctgatat ttttgccact cctcgaacga cagtcgttga
    13321 acctgcggtc tctgcgcctc tagaagctcc cctggtagcg gaacccgtca ttcctccgtc
    13381 tcctatcata ccgaccacgc cagaccaagt ttcgcctcaa gaatcattac aagatttggg
    13441 gataccgccg attgcgtttg aaccggaaat gccggcacaa ccggtaatac gtggagacgc
    13501 ggctccgccg gaacaaatga tcgcccgcat ggaacccgag gcggacaaat cagctcagcc
    13561 aaccccagcg ctgcccgaaa tgattgaaaa gaagacggaa gctctgctca aatctctggg
    13621 agatatttac ttcgattatg accgattttc tattcgggcg gatgcgatct ccgttctgca
    13681 agaaaatgcc caagcactgg cttccggctt ggcgaataac aagattgtga tcgaaggaca
    13741 ttgtgatcag cgtggcacgg aaagttataa catggtgctt ggtgagcgac
//
LOCUS       DATIAL010000248         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1012846, whole genome shotgun sequence.
ACCESSION   DATIAL010000248 DATIAL010000000
VERSION     DATIAL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012846"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VLA60_07710"
     CDS             complement(<1..677)
                     /locus_tag="VLA60_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HSF09283.1"
                     /translation="MYAHRSSIPGVWVAAVLAVCVALPGVTWAKNGPEDKNQGQLTDE
                     ILGQIEIMNDRMHAFSGHGRIRPCDGLAVGGKELTGADRFVTALNGGAYCDQRTGLVW
                     ERSPAGGIFDWAGAISHCATLQLGGWKGWSLPMREQLASLVDINSVLCIGGGLCLPDG
                     HPFQNVKSGGYWSASTTAGTPTFAWVVNFFFGSSLVMNLDANGLAWCVRGDQSSNGKD
                     LAAIELL"
     gene            complement(1531..>2395)
                     /locus_tag="VLA60_07715"
     CDS             complement(1531..>2395)
                     /locus_tag="VLA60_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09284.1"
                     /translation="TTIEIRSALEADSTVVFADPTQLHQVVMNLCANAEFAMRQEGGI
                     LDLTLTSIELTPISVVEFPSLKPGTYLQLTIRDTGQGIPAHVLERIFEPFFTTKDSGE
                     GTGLGLAVVHGVIVGHGGHISVSSTLGQGTTFTILLPRLNVVLPAPPDQAEEWPTGVG
                     RVLFVDDEEMLARWGEQLLTHLGYAVVAKTNPHEAIDLFRKNADQFDLVVTDQTMPAM
                     SGEVFARTLLEIREDIPIILCTGFSHNMTAEKAEQAGLSAFLMKPVNAAVLANTVKNV
                     LEEIQRPLNES"
BASE COUNT          594 a          656 c          564 g          581 t
ORIGIN      
        1 tggagcaact caatcgcggc cagatccttt ccgttagagc tctgatcacc acgtacacac
       61 cacgcgaggc cgttggcgtc caaattcatg acgagcgaac tgccaaagaa gaaattcacg
      121 acccacgcga acgtgggcgt gcctgccgtc gtggacgccg accaataacc cccagacttc
      181 acgttttgga atggatggcc gtcaggcaga catagcccac ctcctatgca aaggaccgaa
      241 ttgatatcta ccagactggc cagttgctcc cgcatcggca gagaccagcc tttccagcct
      301 ccaagctgaa gggtggcgca atggctgatg gcccctgccc agtcaaagat tcctcccgcc
      361 ggagagcgct cccacacgag gccagtcctt tggtcacagt atgctcctcc attcaacgcc
      421 gtaacgaacc ggtcggcacc ggtgagttcc ttccccccca ctgctagccc atcacacggt
      481 cggattcttc catgtccgga gaaggcatgc atcctgtcgt tcatgatctc gatttgtccc
      541 agaatttcgt ccgtcaattg accctgattt ttatcctcgg gtccgttctt ggcccacgtc
      601 actcctggca aggccacaca caccgccagg accgccgcaa cccacacacc tggaatgctt
      661 gatcgatgag catacattct ctgcaccttc ctctgttggt aaaacccatg gcaatattgg
      721 tttgccacac aatttgagaa taacacaaaa ccttctctgc tcacttccaa aaccaggata
      781 ttccgctact gtactctttc cagctcagga agcaagtctt ctctaaagag ggaaatggga
      841 tagaattgta tgtatgagac catggaaacc ggaaagccca accccatgag gagtcgggct
      901 cttggtgttg atgattgagg caggcacttc gtgtgtagaa gatgaagggt ttttggctgc
      961 cagagcgggt taacgggcta ctcctttgta atatcggggt gatcggtaca tgattattga
     1021 gtgtcggacc taggggttca gttctgtcca aggtctgtgg gcggcattcc cctgagttct
     1081 gctccaaaac acaaaccttt aggactgtcc ttaaccgtga ttgagaattt ttctgcttcg
     1141 cccttatccc tctcgctggc tagtgggtgt aattgggaaa caatacctgt ttcaaattcg
     1201 gtgacacacg gataaaaaca tggagtccct cctttgaaga agttgaacaa aaaaaaagga
     1261 cctttgaggc ttaagagccc tcaaaggcct tctattgcct tttcttttac attacaccca
     1321 tcaactttat gatgggatat tgaaaaatcc accacaaatt gaatgagact gtcccaaccc
     1381 ctttcggacc ggcgctgcat aaaaacttcc aacgtatcat cattccccgc caaggaagac
     1441 acctgcgccg gtttgtgaaa ggttacgaag taaaattgaa tctttgaatg aaaatgcaac
     1501 taaatgggat attgatgccc gaaggaacct ctaactttca ttcaagggac gttgaatctc
     1561 ctctagcaca ttcttgaccg tattcgccaa gaccgcggca ttcaccggtt tcataaggaa
     1621 ggcactgagg cctgcttgct cggccttctc ggccgtcata ttatgactga accctgtgca
     1681 gagaatgatg gggatatctt cacggatctc caggagcgtt cgagcaaaca cctcgccgct
     1741 catggccggc atcgtctgat ccgttaccac aagatcaaat tgatccgcat tctttcggaa
     1801 gagatctatg gcttcatgcg gattggtttt cgccactacc gcatatccca aatgcgttaa
     1861 taattgttct ccccatcttg caagcatttc ttcatcatcg acaaatagca cccggccgac
     1921 acctgttggc cattcttcgg cttgatcggg cggtgcagga aggactacat tcagccgtgg
     1981 cagcaaaatg gtgaaggtag taccctgacc cagagtgctg gacacggaga tatgcccccc
     2041 atgtccgaca ataactccat ggacgacggc caagcctaat cctgttcctt cacccgaatc
     2101 tttcgtggtg aaaaatggtt cgaatatgcg ttccaggacg tgggcgggaa tcccctggcc
     2161 ggtatctcga attgtgagct ggagatacgt gccaggtttc aaggagggaa attccacgac
     2221 cgaaattggt gtcagttcga tggacgtcaa ggttaaatcc aaaatcccgc cttcttgtcg
     2281 catggcaaat tccgcattgg cacagagatt catcaccacc tgatgaagtt gtgtcggatc
     2341 ggcaaatacg accgtggagt cggcctctaa cgccgaacga atctcgatcg tggtc
//
LOCUS       DATIAL010000249         3692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1444059, whole genome shotgun sequence.
ACCESSION   DATIAL010000249 DATIAL010000000
VERSION     DATIAL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444059"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="rfbB"
                     /locus_tag="VLA60_07720"
     CDS             <1..1021
                     /gene="rfbB"
                     /locus_tag="VLA60_07720"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HSF09285.1"
                     /translation="EARIVVVDKLTYAGHRENLDGLWDHPRLVFIQADIADAGEMTRI
                     MDAFSPRWLLNFAAESHVDRSIDDPYPFIHTNVTGTLVLLDVVRRFLKQEQADHRQRF
                     RFLHVSTDEVYGTLGPTGLFSEETPYAPNSPYAASKASADHLVRAFHHTYGLPILMTN
                     CSNNYGPYQFPEKLIPLMVLNALEGKPLPIYGNGQNIRDWLFVEDHCEGLLLVLQEGM
                     PGEKYNLGGRSERSNLDVVDALCRILEGVAPGGSNPFLSQRGITRYADLKVFVADRPG
                     HDFRYGIDTTKVYSQLGWSPRHSFESGLQQTIEWYAANRQWCQAVTRNSYQRERLGLA
                     SVPSI"
     gene            1094..1969
                     /gene="rfbA"
                     /locus_tag="VLA60_07725"
     CDS             1094..1969
                     /gene="rfbA"
                     /locus_tag="VLA60_07725"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HSF09286.1"
                     /translation="MKGIILAGGSGTRLYPLTHAVSKQLMPVFDKPMIYYPLSTLMLA
                     GIREILVITTPHEQEGFVRLLGNGSHLGLTICYQVQPRPEGIAQAFVIGREFIGQDRV
                     ALILGDNIFYGHGLSRYLQEAASQATGAQVFAYQVRDPERYGVVTFNDHGQATSLEEK
                     PSRPKSPYAVTGLYFYDNRVVGIADSLKPSARNELEITDVNKTYLEAGSLQVRVLGRG
                     IAWLDTGTHESLMQAAHYIQVLQERQGLMVSCPEEIAYKMGYIQPGDVLRLATKMKDN
                     GYGQYLLHLVNQEQE"
     gene            1976..2530
                     /gene="rfbC"
                     /locus_tag="VLA60_07730"
     CDS             1976..2530
                     /gene="rfbC"
                     /locus_tag="VLA60_07730"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HSF09287.1"
                     /translation="MKFLPTRLPQVLIVEPDVYRDQRGWFLETYHVQKYQEAGILPPF
                     VQDNCSSSVFGTLRGLHFQMTRPQGKLIRVIRGEIFDVAVDIRKGSPTFASWVGVTLS
                     AEDFRQIYVPLGFAHGFCVLSDVAEVEYKCTDVYAPGGEVTIRWNDPRIGIQWPIEQP
                     LLSTKDVAGLLLDDLCDQLPDYQP"
     gene            2527..3363
                     /gene="rfbD"
                     /locus_tag="VLA60_07735"
     CDS             2527..3363
                     /gene="rfbD"
                     /locus_tag="VLA60_07735"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HSF09288.1"
                     /translation="MRQSRILITGAQGQLGQALQRQYADHEVTAWDLQDLDITQFEEV
                     RNALDQLRPHIVINAAAFTQVDEAEVKQDAAYRGNALGPRNLAVVTNDLGMILIHFST
                     DYVFDGQQNRPYHEFDRTNPLGVYGHSKLAGEEAVRVANPQHFIVRTAWLYHTVGKNF
                     PSTICRMAGREVVKVVSDQYGSPTFAPHLAQAVFQLGETDAFGTYHIAGTGGTSWYEL
                     AQTLYQALDIQTSVVPIATAQFPRPARRPSYAVLTSLQDPLIKLPPWEEGVRDFASQW
                     KG"
     gene            3431..>3692
                     /locus_tag="VLA60_07740"
     CDS             3431..>3692
                     /locus_tag="VLA60_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HSF09289.1"
                     /translation="MGYIEAVVLAVIQGLTEFLPVSSSGHLVLAQHWFGHFSETNLLY
                     DIMLHLATVTAILVYFRHDLLTLGLGYVGWTTTQGSLFQGFER"
BASE COUNT          848 a          866 c         1008 g          970 t
ORIGIN      
        1 cgaggcacga attgtcgtcg tggacaaatt gacgtatgcg gggcatcgag aaaatcttga
       61 tggattgtgg gaccatcctc gtctagtgtt tatccaggca gatattgccg atgcgggtga
      121 gatgaccagg attatggatg cattttctcc caggtggttg ctgaattttg ccgctgaatc
      181 gcatgtggac cggtccattg acgaccccta tccctttatt cataccaatg tcacgggtac
      241 gctggtcttg ttagacgtgg tccggcgttt tctgaagcaa gaacaggctg atcatcgcca
      301 acgttttcgg tttctgcatg tttcaactga tgaagtgtat gggacgttgg ggccgacagg
      361 attgttctcc gaagagaccc cttatgcccc caattccccc tatgcggcgt cgaaagccag
      421 tgctgatcat ttggtgcggg cttttcatca cacatacggt cttccaatcc ttatgactaa
      481 ttgttccaat aactatgggc cctatcaatt tcccgaaaag cttattcctc tcatggtctt
      541 gaatgcctta gaagggaagc ctcttccgat ttatgggaac gggcagaata tacgggactg
      601 gttgtttgtg gaagaccatt gtgaaggcct actcctggtc cttcaggaag gaatgcctgg
      661 agaaaaatat aacctgggcg ggcgatcgga acgatcgaac cttgatgtcg tggacgccct
      721 atgccggatc ttggaagggg ttgccccggg tggttccaat cctttcctat cccaacgggg
      781 tatcacacga tacgcagacc tgaaggtatt tgtggctgac cgtcccgggc atgattttcg
      841 ttatggcatt gataccacca aagtttactc gcaattgggg tggagcccac gccactcttt
      901 cgagtctggg ttgcagcaga caattgaatg gtacgctgcc aatcgccaat ggtgtcaggc
      961 cgtgaccagg aattcctatc aacgggagcg actcgggtta gcctctgtcc catcgatata
     1021 ggggcgggaa ctttctctca ttttggcaag gtaacatgag gataatgaag acattgggat
     1081 gcaaaggggg acgatgaagg gaatcatcct agcgggtgga tcgggaaccc gactctatcc
     1141 cttaacccac gcggtcagca aacaactcat gccggttttt gataaaccga tgatctatta
     1201 tccattgtcg actctcatgc tggctggaat ccgcgaaatt ttggtgatca ccactccgca
     1261 tgagcaggaa gggtttgtcc ggttgttagg taatggcagt catctcgggt taacgatctg
     1321 ctaccaagtg caaccccgac ccgaaggaat tgcgcaagcc tttgtgattg ggcgtgagtt
     1381 tattggacag gatcgggtgg cgcttattct tggggataat attttttatg ggcatgggct
     1441 gtctcgctat cttcaggaag cggcctctca ggccacaggg gctcaagtct ttgcctacca
     1501 ggtacgggat cccgaacgat atggtgtggt gacctttaat gatcacgggc aagccaccag
     1561 cctggaggaa aagccctccc gtcccaagtc gccctatgcc gtaaccgggt tatattttta
     1621 tgacaatcgg gtcgtgggta tcgccgattc tcttaagcct tcggcgagaa atgaactcga
     1681 aatcaccgat gtgaataaaa cctatttaga agctggatcg ctgcaggtgc gtgtgcttgg
     1741 gcgtggcatt gcctggttgg atacggggac tcacgaatct ctcatgcaag cggcccatta
     1801 tattcaagtt ttacaagaac gacagggcct gatggtttcc tgtccggaag aaatcgcata
     1861 caaaatggga tacattcagc caggtgacgt gttgcgctta gccacgaaaa tgaaagacaa
     1921 cgggtacggg caatacttgc tgcatttggt gaatcaggag caggagtaac cggatatgaa
     1981 atttctccca acgcggctcc cgcaggttct gattgttgag cctgatgtgt accgggacca
     2041 acgtggatgg tttttagaaa cgtatcatgt gcaaaagtat caggaggcag ggattctgcc
     2101 tccatttgtg caggataatt gttcatcgtc ggttttcggg acccttcgtg ggctccactt
     2161 tcaaatgact cgcccacaag ggaagctcat tcgggtaatc cgtggagaaa tttttgatgt
     2221 cgcggttgat attcgaaagg ggtctccgac ctttgcctca tgggtggggg tgaccttatc
     2281 ggcagaggat ttcagacaaa tttatgttcc tttgggattt gcccatggat tttgtgtcct
     2341 aagcgatgtg gcggaagtgg agtacaaatg taccgatgtc tatgcacctg ggggagaagt
     2401 gaccattcga tggaatgatc cccgtattgg cattcaatgg ccgatcgaac agccacttct
     2461 ctctacaaag gatgttgccg ggctgttact tgatgacctg tgcgaccagc ttccggatta
     2521 tcaaccatga ggcagagtcg aattttaatc acaggggctc aagggcaatt agggcaggcg
     2581 ctccaacggc aatatgccga ccacgaggtc acggcctggg accttcagga tttggatatc
     2641 acgcagttcg aggaagtcag gaacgccctt gatcaactac gaccacacat cgtcatcaat
     2701 gcagccgcat ttacgcaggt agatgaggca gaagttaagc aggatgccgc ataccggggc
     2761 aatgccctgg gcccgagaaa tcttgccgtg gtgacgaatg atttggggat gattcttatc
     2821 catttttcca ccgattatgt gtttgatggt cagcagaatc ggccctatca tgaatttgat
     2881 cgtacgaatc cgttaggcgt ctatgggcat agtaaattag ccggtgaaga ggctgtgcgg
     2941 gtcgcgaacc cacagcattt tattgttcgg acagcttggc tttaccacac tgtagggaag
     3001 aacttcccca gtacgatttg tcgaatggcg ggccgagagg tcgtgaaagt cgtcagcgac
     3061 cagtatgggt caccgacctt tgcccctcat cttgcccagg cagtatttca attgggtgag
     3121 accgatgcgt ttgggaccta tcacatagcc ggtacaggcg gaaccagttg gtatgaattg
     3181 gctcaaacgc tttatcaggc gttggatatt caaacctcgg ttgtgccgat agcgacggct
     3241 caatttcctc gcccggctcg gcggccctcc tatgccgtgc tgacttcttt acaggaccct
     3301 ttgattaaat tgccgccctg ggaagagggc gttcgtgatt ttgccagtca gtggaaaggc
     3361 tagggtcgtc atctgttctg tgaatcatct tttccctgat catcagctat aagaaaggca
     3421 ttcctaaacg atgggttaca tcgaagcggt ggtgcttgcg gtcattcaag gactcacgga
     3481 attcctcccg gtatcctcat ccggacattt agtcctggct caacactggt ttggtcattt
     3541 tagtgaaacc aatttgttgt atgacatcat gttgcatctc gcgacggtga ctgcgatcct
     3601 ggtgtatttt cgtcatgatc tcctcaccct gggcttgggt tatgttggat ggacgacaac
     3661 acaaggcagc ctgtttcaag gatttgagcg ac
//
LOCUS       DATIAL010000250         4501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_261651, whole genome shotgun sequence.
ACCESSION   DATIAL010000250 DATIAL010000000
VERSION     DATIAL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261651"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..420)
                     /locus_tag="VLA60_07745"
     CDS             complement(217..420)
                     /locus_tag="VLA60_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HSF09290.1"
                     /translation="MKVKYFSDTDTAYIEFSTHAVAETRDINDNILLDLDKVGQLVGM
                     TIEHADTQANLSEFSFQQLTPKS"
     gene            complement(495..653)
                     /locus_tag="VLA60_07750"
     CDS             complement(495..653)
                     /locus_tag="VLA60_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09291.1"
                     /translation="MAVKDIAKQVIDVLPDEATMDDIIHALYVKIKFEHGEREIREGK
                     GVPNEAAK"
     gene            complement(717..1010)
                     /locus_tag="VLA60_07755"
     CDS             complement(717..1010)
                     /locus_tag="VLA60_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HSF09292.1"
                     /translation="MPPTVLRWAGHVLTGPNVCPTFHFMKSTSITIRLDKDLDRLLRE
                     ACRQSGKSRSTLAREALTRHLRLVQFEALRKRTMPFAEARGYLSDEDLFRNNS"
     gene            1720..2589
                     /locus_tag="VLA60_07760"
     CDS             1720..2589
                     /locus_tag="VLA60_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF09293.1"
                     /translation="MNVKISQPPASQHHTEPPTPATEAGPSDTKEIIYLASGLLILVL
                     GIGGLLMYSEETPLPATTSQGIDKAQMASAFTSSKQQPASDEPLALSQALAEKPVTNT
                     IIPATGPGENQPIAETDVYFAFNESTLSDEAKDSLKTRMENRPEGWSGTLRIVGHTDT
                     QGPDAYNRALGLKRAQSVKTFLMSLGVAEDDVQVDTLGKDGSICQEETTECFEQNRRA
                     HVALLPASPSEGEDLQLSMTPADTDSPADEELVLTTDESPIVSSDTEVSFQEEVQEES
                     VSSDPLLTVESLP"
     gene            2690..>4501
                     /locus_tag="VLA60_07765"
     CDS             2690..>4501
                     /locus_tag="VLA60_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HSF09294.1"
                     /translation="MNFWKQLLLPLAFWLTGSVVDCLGLPPVSAQPLKPDVTAPSQQA
                     GPDISHGLSIRPMSGGPILPALTVSTHVTTTISGLIVRTTVSQTFHNPSSEWAEGIYV
                     FPLPEQAAVDHLRMRIGERVIEGMIQERAEARKTFDQAKRKGQRASLLEQERPNVFTA
                     SVANLAPGEPIIVEIEYQDTVLYDQGRFSLRFPMVVGPRYIPGTPLSLVEAQPHEPGE
                     GWAVNTDQVPDASRITPPVQHPGEGLLNPLTLQIDLAPGFLLDRVESPTHLIQVETHS
                     NGRLHITLADPSTFADRDFELTWTPQASHEAQTQLFLEEHDGDTYGLVFFLPPKINEA
                     GPGDIPREVIFVIDTSGSMAGASLVQAKAALTLAVSRLTPRDRFNVIQFNSITKHLFP
                     LAVPAETRAIQHARSYVNSLQAEGGTEMLLAVVQALSHQKEEQTLLRQVIFITDGLIG
                     NEDEFFATLHRLLGQTRLFTVSIGSAPNGHFMRKAAQHGRGTFTYIGTTQEVQDKMQR
                     LFVKLEQPAFLNLALEGSAGAAWDLLPSPLPDVYAGEPLMATFRTTTPPAHLTISGMH
                     GTVPWKRVLPFAAGLSRSGIAVHWARQKISQLMDHQAS"
BASE COUNT         1065 a         1318 c         1114 g         1004 t
ORIGIN      
        1 gcccggcatt actgaaatcc ccttggacac gagagaaagt tggtaccttc acatacgcag
       61 gtcttcccga cttcggtttt tgcctggaaa aaaccaagat ggattcccgc taacaaccgg
      121 cgggaatgac gaaaaaaagg agagatccct acctggatcc ccagatgtga ataccaaagg
      181 gtgggctgaa attccaatga gacaaggagg ctacggttaa ctttttgggg tgagttgttg
      241 gaaagaaaat tcagaaaggt tcgcttgggt atcggcgtgt tcaatggtca ttccgacaag
      301 ctggccaacc ttatccaggt caagcagaat attgtcattg atatcccggg tttctgccac
      361 ggcatgggta ctaaactcaa tatacgccgt atctgtatca gaaaaatact ttactttcat
      421 tctggcctcc taaaagatcg gtcaaaaaaa gcatgcagtt ggaaacaaaa tcacttgacc
      481 cattttccca gcctctattt ggccgcttca ttgggaaccc cttttccttc gcggatttca
      541 cgctcaccat gttcaaattt aattttcacg taaagggcat ggataatgtc atccattgtg
      601 gcctcatctg gtaatacgtc aatgacttgc ttggcaatat ctttgacagc catgttgctc
      661 ctccttctta gaaactctct tctctgtttt ccacgatatc gtttcatccc accgcattac
      721 gaattattgc gaaagagatc ttcatccgaa agatagcctc gcgcttccgc aaacggcatg
      781 gtccttttgc gcaaagcctc gaattgcacg agtcgcagat ggcgggtaag ggcttcacga
      841 gctaacgtgc ttcgggattt tcctgattgg cgacaggctt ccctgagcag ccgatcgagg
      901 tccttgtcca gtcgaatagt aatggatgtg gatttcatga aatgaaaggt aggacagaca
      961 ttaggccctg tcaatacatg ccctgcccac ctgagcaccg ttggtggcat ggacacagat
     1021 tcttctcggg tagcttagaa tgacaattta gttggtctct cttcgacgcc tgccgcccgt
     1081 ccccctgacc ggacgctctt gttcttgacg ggcttgtgtg agcgcagcga gttagcccgt
     1141 ctccccttct tcgcgtccga tcaatccgat tcagccagac ggggcgtcaa tggttttgcc
     1201 cacttttgcc gaaacaaaag tgggtcggct gccggggcga aacccggcaa cccagaacat
     1261 cacgccgaca ctaggacgac aggaatgacc aaaaaacggt catgggtgtc ttgtgtgaag
     1321 agggaaaaac tctacctccc ctaaccccgc cttacaaagg cggggaatct gcaaaaaaag
     1381 attgccctct taagaccgta gctatgatca agggttgaag cttccggttg attcttcttg
     1441 gccgacgatg cggggatatc cccgcatcca ctcattcgtc cccgcaatcc ccgcaaatca
     1501 agtaacggca catcacacct aagccactgt ttttaaacaa cttttcaaat ccatcgtcat
     1561 ggcaagcccc ttgcgatagg acggtttcaa caatcgcatt cacaaccgga aaggaggtga
     1621 acattcacag gtgctaaagg gcggattttc aaatgagttc gcaaaacaag acgaccggaa
     1681 atgccaacga caacttaacg acttcacaag gagacagcca tgaacgtgaa aatttctcag
     1741 ccacccgcat cacaacatca cacggagccc ccaacccctg cgaccgaagc cggtccatcg
     1801 gataccaagg agattatcta tctcgcctcc gggttactca tcctggtctt ggggataggc
     1861 ggcctcttga tgtattccga ggaaacgccc ctgcctgcga ccacttctca gggaatagat
     1921 aaggcgcaaa tggcgagtgc gtttacctcc tccaaacagc aacccgcttc tgacgaaccc
     1981 ctggcattga gccaggcctt agctgaaaag ccagtgacca atacgatcat ccctgccacc
     2041 gggcctggtg agaaccagcc gatagcagag acggatgtct attttgcgtt taacgagtcg
     2101 accttgtccg atgaagcaaa ggacagcctc aaaacccgaa tggagaacag gccggaggga
     2161 tggagcggca ccctccgcat cgtgggacat accgacactc aggggcctga tgcctacaac
     2221 cgtgccttgg ggctcaagcg agcccagtct gtcaaaacct ttttaatgtc tcttggcgtg
     2281 gctgaggatg acgtccaggt ggacacgttg gggaaagacg gatcgatttg ccaggaagag
     2341 acgacggaat gttttgaaca aaaccgacgg gcccatgtgg cgttacttcc tgcctcacca
     2401 tcagaaggag aagacctcca actgtcgatg actcctgccg acacggacag cccggccgat
     2461 gaagaactgg ttctgaccac tgacgaatca cccatcgtct cttcggacac tgaagtctcc
     2521 ttccaggagg aagttcaaga ggaatcggtc tcttccgatc cactcctcac cgtcgaatcc
     2581 cttccctaag gctctcaacc cgggttggct caccctgctg tgggtcggcc cgggcccgcc
     2641 ctgacggaca gttttagcca tcacctttta cgacgataaa ggagccggta tgaatttctg
     2701 gaagcaactt cttctgcctc ttgccttttg gctcaccggc agtgtggtgg attgcctggg
     2761 ccttcctccc gtgtccgctc aaccactgaa acccgacgtc acggcaccct ctcaacaggc
     2821 cggaccggac atatcacatg gcttgtcgat tcgccccatg tcgggaggac ccattctccc
     2881 cgccctcacc gtttccaccc atgtcactac gaccatcagc gggctgatcg tccgcaccac
     2941 cgtctcgcaa acctttcaca atcccagttc cgaatgggcc gagggcatct atgtctttcc
     3001 gttacccgag caggcggccg tcgatcacct gcgcatgcgg atcggcgagc gggtgattga
     3061 agggatgatc caggaacggg ccgaagccag gaaaacattt gaccaggcca agcgcaaggg
     3121 acaacgcgcc agcctgctgg agcaggaacg acccaatgtg tttaccgcgt ccgtggcgaa
     3181 tctcgcgccg ggtgaaccca tcatcgtgga gattgaatat caggatacgg tcctgtatga
     3241 ccaaggccgg ttttctctcc ggtttccgat ggtggtcggc ccacgctata ttcccggcac
     3301 tcccctatct ttagtcgagg ctcaaccaca cgaaccgggc gaggggtggg cggtcaatac
     3361 cgatcaggtt cccgatgcct cacggatcac gccgcccgtg caacatcccg gtgaaggcct
     3421 tctcaatccc ctcaccttgc aaatcgatct ggcacccggt tttctgcttg atcgcgtgga
     3481 gtccccgact cacctcattc aggttgaaac ccattccaac ggacgcttgc atattacctt
     3541 ggccgaccct tccacgtttg ccgatcgcga tttcgaattg acctggactc cgcaagcttc
     3601 gcatgaagcc caaacacaac tgtttctgga ggaacacgac ggcgacacct acggtctggt
     3661 gtttttcctt ccgccgaaaa taaacgaagc cgggccgggt gacataccgc gtgaagtcat
     3721 ctttgtgatc gacacctcag gatccatggc cggcgcatcg ctcgtgcaag ccaaagccgc
     3781 ccttacactg gccgtgtccc ggctgactcc cagggaccga ttcaacgtca ttcagttcaa
     3841 ttccatcacg aaacacttat ttccactggc cgtgccggct gagacccgcg caattcaaca
     3901 cgcgcgttcg tatgtgaaca gccttcaagc tgagggcggg acagaaatgc ttctggctgt
     3961 ggttcaagca ttgtctcatc aaaaagaaga acagactctt ctaaggcagg tgatctttat
     4021 caccgacggg ttgatcggca atgaggatga attcttcgcg acccttcacc gactgctcgg
     4081 ccagacccgc ttgtttaccg tcagcatcgg ctccgcgccc aacggacatt tcatgcggaa
     4141 ggccgctcaa cacggccggg gcacctttac ctacatcggc accacacaag aggtgcaaga
     4201 caagatgcag cgcctgtttg tcaaactcga acaacccgca tttcttaatc ttgcgctgga
     4261 aggttccgct ggtgcggcat gggatctcct tccgtctcca ctgccggatg tgtatgcggg
     4321 agaaccgctg atggcaacgt ttcgaacgac aacccctccc gcccatctga ccatttccgg
     4381 catgcacgga accgttccat ggaaaagggt actgccgttc gcggccgggc tttcccggtc
     4441 gggcatcgcg gtccattggg ccaggcaaaa gatttcacaa ctcatggacc accaggcttc
     4501 a
//
LOCUS       DATIAL010000251        10987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_906005, whole genome shotgun sequence.
ACCESSION   DATIAL010000251 DATIAL010000000
VERSION     DATIAL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906005"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VLA60_07770"
     CDS             complement(<1..218)
                     /locus_tag="VLA60_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09295.1"
                     /translation="MKAKTLGILAAITIAGIVLAIFVNQKPASQFPQSGELLFPELLS
                     VVNDVSEIVVETKDQTVTLVREENIWRV"
     gene            complement(277..2211)
                     /locus_tag="VLA60_07775"
     CDS             complement(277..2211)
                     /locus_tag="VLA60_07775"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HSF09296.1"
                     /translation="MMNKKVLTGSGLIMAAVLFGVFNMVSNAAFSSARFDLTEHGLYT
                     LSKGTKNVLESLKEPITLRFYLSKALATGLPGIKSYATRVQEMLEEYAQVAGDRLYLE
                     VIDPEPFSEEEDRAVAFGLQGIPLDGGSTQFYFGLAGTSSTDELEVIPFFQPEREEFL
                     EYDLTKMVHTLANPKKKILGLLSTLPIDGSGGMSFMAQQGGSQPWLILSHIEQMFEVK
                     KIETTAAAIPEEISVLMIVHPKSLSEATLYAIDQYVLRGGHAMIFVDPLAESDSGGGN
                     PMNPMGGGGPRNSDMPTLFAAWGLELVKGQVLGDLPLAKKVQVQQQNRLQVVDYPVWI
                     DFRQEHFSDKDIVTAQVPSITVASAGIIRKKGETGTTVEPLIQSDEAAMQIEASRLSV
                     MPDVSGLLNSYRPEGEKFIVAARVTGTVKTAFPEGKPKVAANTSENPDMPSEAETQTK
                     DHLTESKGSINVIVVADTDILQDRFWVQVQNFFGQRIGIPNSGNGTFVTNALDNLTGS
                     NDLISVRSRAGYSRPFTLLRMLQQEAEQRFRQKEQVLQEQLKATERKIQELQSQKPEG
                     NAMILSVEQQEAMGQFRKELLQVRKELRGVQHELGKNIESVERWVKFINIGLVPLLIG
                     IVGVWISSSGIRKKGSPKPK"
     gene            complement(2259..2996)
                     /locus_tag="VLA60_07780"
     CDS             complement(2259..2996)
                     /locus_tag="VLA60_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HSF09297.1"
                     /translation="MGRIRVIFQRELAGYFATPIAAVFIVIFLLLSGAFTFYLGNYFA
                     RGQADLVPFFDFHPWLYLVLIPALAMRLWAEERRSGTIELLLTLPVTMWEAVAGKFLA
                     AWCFTGIALALTFPMWLTVNLLGDPDNGVILASYLGSLLMAGGFLAIGSCISALTKNQ
                     VIAFVISVVISLGFILSGFPLVLELFSGWAPQFLLSAISSFSFLTHFQSISKGVLDLR
                     DILFFLSLIAFWLFATATVIDIKKAES"
     gene            complement(3153..4109)
                     /locus_tag="VLA60_07785"
     CDS             complement(3153..4109)
                     /locus_tag="VLA60_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09298.1"
                     /translation="MDIMVKIENLRKTFGPIVAVDGVSFTVGKGEVLGFLGPNGAGKS
                     TTMKMITGFLTPTSGAVHICGQNIDVQPINAKKRIGYLPEGAPAYQDMTPASFLTFIG
                     EMRGYRGATLKRTVSETVEKVNLQSVLNQSIETLSKGFKRRVGLAQAILHDPEILILD
                     EPTDGLDPNQKHEVRTLIRAMAKEKAIILSTHILEEVHALCTRAMIIAKGRVVFDGTP
                     AELEGKSPTHHTVLGTIAGVDPMVLHHHLMTVKGVKKIEELGRDGDLVQLRIYPDDGE
                     WILPDIGHCAREQGWEVRELYPDRGQLDEVFRLLTTPKQTIS"
     gene            complement(4198..5967)
                     /locus_tag="VLA60_07790"
     CDS             complement(4198..5967)
                     /locus_tag="VLA60_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HSF09299.1"
                     /translation="MMVCLGLMGLATPVSAQSVRAYVDRNPVMADETFQLIVETDQTS
                     SGESPEWHILDADFEVLGTTQSQQTSIINMRTTSLVRWIATLAPKRTGAVTIPPIHVG
                     AHQTGPIQLEVNEPNHTREANESQDIFLEADVDSHVSYLQGQVLLTLRLVSAISVQEG
                     RLDEPEMDWGIVERVGKDVSYETSRNGRRYHITERRYVITPQQSGKQVIPPILFSGTV
                     QEGRARGTLFEELFGNRPGSLGRDPFTTSRSIHRRSPKIAINVKNFPSDLNGRFWLPA
                     KNLTLTETWSGDTDTLQIGDSPTRTIVMRAIGQRGEQLPEVTVPPQPIVKIYPDKAKT
                     QTDFDGKWIVGSREEKYVFVPTQPGTVTLPAIHVPWWNIETDQWEEATLPAKVLTVLG
                     TRQPASSGQIPTSVPDLPVSSTESDRQQMDVQFGTSDSDRPLPWPWITGGCFTLWLVT
                     LVAWWRERQKRNGRGQDMAKRNDGKFESEQNAIQEVKAACFEQSAEKTRTALLQWASL
                     KEKGRPCRSLKSVERLLSQPVPDPAAISAAIWNLDRTLYTTSAEKQNWDGRQFWETVK
                     PAITAKPPKSHKHVKELPPLYLH"
     gene            complement(5991..7904)
                     /locus_tag="VLA60_07795"
     CDS             complement(5991..7904)
                     /locus_tag="VLA60_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSF09300.1"
                     /translation="MAMFHFLRPEWLWGLLPLFGLFLLVARKGTTDQIWESVCDAHLL
                     PHLLVGTEGATRRMPLVLLGSGWIVAVCALAGPVWSQLPQPVFRAESALVMVLDLSRS
                     MDAQDVTPSRLTRAKHKILDLLKARKEGQTALVVYAGESFVVSPLTNDANTIVTLVQS
                     LETELMPLQGSRTDLALQKAHKLLQHVGQARGDVLLLTDGEGDPATLKVVGELHRQGV
                     RVSVLGIGTVDGAPIPETGGFLKDQEGTVVIPKLDIPSLQAIARAGGGHYATVTADDH
                     DINRVFPSIVPTRALSSSTSEQRTTDLWREEGPWLVLLLLILALPAFRPGWLGMLLAF
                     LLIPQVSEAFSWENLWVRPDQQGIQALERDAPEEAAALFQDPGWKGIAHYRAGKYQEA
                     EEAFSIMDTPEGHYNRGNALANLGRYEEALASYQTALTQQPDHADAQHNLEIIKQLLD
                     ESSSAEQAEESPQQSDGSSAKQNNNGNEAGGEGEKSENPSDPTHKQSDSKTADSQKEK
                     QEEAAQMAGSGSDSARTSNQSNGDQKSSEENIEREAQGLDPLKAEQSKKPRSTPTTME
                     EKSEDQVEHSEVSSAGDAAHSPEIEKSEQVLQQWLRRIPDDPGGLLRRKFLLEHQRRV
                     EAGKSTISQGNRW"
     gene            complement(7906..8961)
                     /locus_tag="VLA60_07800"
     CDS             complement(7906..8961)
                     /locus_tag="VLA60_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HSF09301.1"
                     /translation="MITLAWPWVLALILCPWMVRRWATPVSISSGRAMKVPHFDDIMA
                     LQSNQLIGPTRSRHSTLFWAGIFIWAALVLAAARPQWSGEPVGLPTSGRDLMLAVDVS
                     GSMKIRDFSVKGQEVTRLEVVKAAAGEFIAGRTGDRIGLIVFGSQAYVQTPLTFDRDT
                     VKAMLTETEIGLAGQETAIGDAIGLAVKRLKEQPAGSRVLVLLTDGANTAGEVSPTQA
                     AALAEEQGIRIYAIGVGADRMEIESFFGTQTVNPSRDLDEDTLRHLAQRTGGLYLRAK
                     DTEGLTRVYEELDRLEPAATETELFRPTTELYIWPLGFALALSCVMALSFIWNINWVL
                     RSRWRAEAISAQAGAGR"
     gene            complement(8958..9455)
                     /locus_tag="VLA60_07805"
     CDS             complement(8958..9455)
                     /locus_tag="VLA60_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 domain-containing protein"
                     /protein_id="HSF09302.1"
                     /translation="MPTASSPLQELRDVHLPPPISLWPPAPGWWIIFGLVMMGVVLGL
                     WILRNRRRKQSCRLAMNELRVIKQHYDTHRDDQWLIQRLSVMIRRYAIATFPRNEVAG
                     LVGISWLQFLDRSGRTNQFTDGVGHLLSSGPYQQQAAVSAAELVPLVEQWIQQVTPPT
                     GKGTA"
     gene            complement(9471..10478)
                     /locus_tag="VLA60_07810"
     CDS             complement(9471..10478)
                     /locus_tag="VLA60_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HSF09303.1"
                     /translation="MWPFRKQTTPPEFRLNRPTGFQGVEVQLADLINMRHQTGVLGIK
                     TRKRVHTLLAGGERSPFKGRGMDFEESRRYQPGDDIRLMDWRVMARTHEPYLKVFREE
                     RERPVFIVVDNRRGMRFGTKVAFKSVIAAHAAALLGWASQERGDRVGGVVFSDVDHVE
                     LRPRGGRTGVLQLLNILAQDSANPSQMIERQSPITSSFQLALNRVQATAKPGSLIFLL
                     SDFRDWEHQAKQTLIRLGGHQDVVAIFMYDQLEQEPPPAGQYPVTDGTRMGILNTGSA
                     NTIQSYSACFRERFEDVRTLCLTRGIGFIPLGTHDDILFQLRGGLQDLGHRRSAHHSM
                     A"
     gene            complement(10462..>10987)
                     /locus_tag="VLA60_07815"
     CDS             complement(10462..>10987)
                     /locus_tag="VLA60_07815"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HSF09304.1"
                     /translation="IEQEGTYPLPEAQLDRFLLYVRVQYPDVEGERKIVELVRRQARA
                     IPGEVTTAPTPVPQKVIVAARRQMWDIYVAPALEEYIVQLVMATREPGPYSSKLRRWI
                     RFGASPRATIGMERCARAHAWLEGRDYVSPEDIQAVAHDVLRHRVLLTFEAEAEGIRT
                     DQVISEILNHVAVP"
BASE COUNT         2594 a         3111 c         2588 g         2694 t
ORIGIN      
        1 ttaacccgcc agatattctc ctcacgcacc agcgtcacag tctgatcctt agtctccaca
       61 acaatttcgc tcacgtcatt gacaaccgac aataattccg ggaacaacag ctcaccactc
      121 tgcggaaact gagaggccgg cttttgattc acgaaaatgg ctagaacaat cccagcaatg
      181 gttatggcgg ctaagatgcc tagagttttg gctttcatat gcttccctta atattcttga
      241 atagtcgaat aacgtccagg ttgatcgata tctgagttac ttaggttttg gggacccctt
      301 ctttctaatc cccgaagaac tgatccatac cccgacaata ccgatcagca acggcacgag
      361 cccgatattg ataaatttta cccaacgttc cacactctca atgtttttgc caagctcatg
      421 ctgtacgcca cgcagctctt tgcgaacctg aagcaattcc tttcgaaatt gtcccatggc
      481 ctcctgctgt tcaacactca gaatcatggc atttccctca ggcttttggc tctgcagttc
      541 ctgaatttta cgttccgttg ccttgagctg ttcctggagc acttgctcct tttgccggaa
      601 gcgctgctcc gcttcctgtt gcaacattcg caaaagcgtg aatggccggg aatatcccgc
      661 ccggcttctc acactaataa gatcattgct tcccgtcaga ttatccaagg cgtttgtcac
      721 aaacgtgcca ttgccagaat tgggaatgcc tatccgttgg ccaaagaagt tttggacttg
      781 aacccaaaac cggtcctgca agatatccgt atcggccacc acaatcacat tgatggaacc
      841 ttttgattct gtgaggtgat ctttggtctg ggtctcggct tctgaaggca tatccggatt
      901 ctcacttgta ttcgccgcca ctttaggctt tccctcagga aatgccgttt tcacggttcc
      961 cgttaccctg gcggccacaa taaatttttc tccttcaggc cgataactgt tgagtaaacc
     1021 gctgacatca ggcatgacgg ataaacgtga ggcctcaatc tgcatggcgg cttcatccga
     1081 ttggatgagc ggttcgaccg tggtccccgt ctcccccttc tttcgaatga tgcctgctga
     1141 tgccaccgtg atcgacggca cttgagccgt gacaatatcc ttgtcgctga aatgttcctg
     1201 gcggaaatca atccagactg gataatctac cacctgtaga cggttttgct gttggacttg
     1261 cacctttttg gccaacggca aatctcccaa cacttgtcct ttcaccaact ccagacccca
     1321 ggcggcaaat aacgtaggca tatccgaatt ccgggggccg ccgcctccca tcgggttcat
     1381 ggggtttccg cctccactat cggattccgc aagaggatcc acaaaaatca tggcatggcc
     1441 gcctctcaaa acatactgat caattgcata gagagtggcc tcactcaggg acttaggatg
     1501 cacaatcatt aagacactaa tctcctcagg gatagccgcc gccgtcgttt caattttttt
     1561 cacttcgaac atttgttcga tatgagagag aatgagccag ggttggcttc ctccctgctg
     1621 tgccataaac gacatccccc cactcccatc aatgggcaag gtgctcagca ggccaagaat
     1681 ttttttcttg ggattggcca acgtgtgaac catcttcgtc aagtcatact ccaaaaactc
     1741 ttcccgctct ggttgaaaaa aaggaatcac ttccagctca tccgtcgaac tggtcccggc
     1801 caatccaaaa taaaattggg tgctcccacc atccagcgga ataccctgca gaccaaacgc
     1861 gaccgctcgg tcttcttctt ctgaaaacgg ttcaggatct atcacctcca agtacagtcg
     1921 gtctcccgcg acttgtgcat attcctccaa catctcctga acgcgtgttg cgtaactttt
     1981 aatgcccggc aagccggttg ccaaggcttt agagagataa aaccgcagtg taatgggttc
     2041 cttcaaactc tcaagaacat tcttggtgcc tttagacaag gtatacaggc catgttctgt
     2101 cagatcgaat cgtgcagaac tgaacgcggc attactcacc atgttgaaga cgccgaacag
     2161 aacggctgcc atgatgagac cgcttcccgt gagtactttt ttattcatca tcactcctca
     2221 tttgcaaagt ttcacaccgg atccttcaaa ccgtcccctt acgattcggc ctttttgata
     2281 tcaatgaccg tggccgtcgc aaacaaccaa aaggcaatca gcgagaggaa aaagagaata
     2341 tcccgcaaat ccaagacacc tttgctgatc gattggaaat gcgtcaaaaa gctaaatgag
     2401 ctgatggcac tcagcagaaa ttgtggcgcc cacccgctga atagttccag aacgagtggg
     2461 aacccactca agatgaaccc caaagaaatg acaacgctga tcacaaaggc gatcacctga
     2521 ttcttggtga gggcggagat gcaggagccg atagctaaaa atcccccggc catcagcaga
     2581 ctgccgaggt agctggcaag gataacacca ttatcaggat cgcccagtaa attcaccgtg
     2641 agccacatgg gaaaggtcag agccaaggcg attcccgtga aacaccatgc ggccagaaat
     2701 ttccctgcga ccgcctccca catggtgacg ggcagggtca gcaacagctc aatcgtcccg
     2761 gaacgacgct cttccgccca taagcgcatt gccaacgcag ggattaacac cagatagagc
     2821 cacggatgaa aatcaaaaaa cggcaccagg tcagcctggc cgcgcgcaaa gtaatttccc
     2881 agataaaacg tgaaggctcc actgagtaac aaaaatatga caatgaacac cgccgcgatc
     2941 ggcgtggcaa aatagccggc caattcccgt tgaaaaatca ctcgtatacg tcccacaact
     3001 cctccttcac tctcgaatac gtctttcagt taatctcttc caaggccatt gggccctcgg
     3061 agtcacataa agatgggatc ccgttggttt gaggattttc tgccgaagca gcccacccgc
     3121 ctaaaatttg catccttcta aaaccaggcc tattacgata tcgtctgctt gggagtcgtc
     3181 aataagcgaa atacctcatc caattgtccc cggtcgggat agagttccct cacttcccac
     3241 ccctgttctc gcgcgcaatg tccaatatcc gggagaatcc actctccgtc atccgggtaa
     3301 atacgaagtt gcactaagtc accgtcccgg cccaactctt caattttttt gacacctttc
     3361 accgtcatca ggtgatgatg taagaccatg ggatccacgc cggctatcgt gccgaggacc
     3421 gtatggtgag taggagattt tccttccaat tccgctggtg tgccatcaaa caccactctc
     3481 cccttcgcaa tgatcattgc gcgcgtacac agtgcatgta cttcttccaa gatatgcgtc
     3541 gagagaataa tggccttctc cttcgccatg gcccgaatca gcgttcgaac ctcatgcttt
     3601 tgattgggat ccaatccatc ggtcggttca tctaaaatca aaatttccgg gtcatgaagg
     3661 atcgcctgag ccagtcccac gcggcgctta aaccctttcg acagggtttc aatcgattgg
     3721 ttcaacaccg attgcaaatt caccttctcg accgtttccg acacagttcg cttaagcgtc
     3781 gctccacgat atcctcgcat ctccccaata aacgtaagaa acgaggctgg agtcatatcc
     3841 tgataggccg gagctccctc tggcagatag cctatacgct ttttggcatt gatgggttga
     3901 acgtcaatat tttgcccaca aatatgcact gcgccgctgg tcggcgtgag aaaacctgtg
     3961 atcattttca tggttgtgga tttacctgct ccgttcggcc ctaagaaccc gagcacttct
     4021 ccttttccta cggtaaacga cacaccatcc actgcgacaa tcggcccaaa tgtttttcgc
     4081 aagttttcaa ttttaaccat gatgtccatg ctaccctctt tatctcaata atttcatgaa
     4141 gtgatttgtg ggacctgttg tgctctcgct ttctgattta atcgaccccc tttaaaatta
     4201 atggagatac aatgggggca actcttttac atgtttgtga gatttcggtg gcttggctgt
     4261 tatggccggc ttgactgttt cccaaaactg ccgcccatcc cagttctgtt tttctgccga
     4321 ggtcgtgtat aaggtccgat caaggttcca aatggccgca gaaattgctg ccgggtcagg
     4381 aaccggctga ctcaagagac gttccactga tttcaggctt cgacagggac gccctttttc
     4441 cttgagggaa gcccattgta agagtgccgt tcgcgttttt tccgccgact gttcgaaaca
     4501 agcagccttg acctcttgaa tggcattttg ttccgattca aatttcccat cattcctttt
     4561 tgccatatct tgacctcgtc cattccgttt ttggcgctcc ctccaccagg ccaccagggt
     4621 caccaaccac aacgtgaagc atccccctgt tatccacggc catggtaacg gcctgtcact
     4681 atcagaggtg ccgaattgga catccatctg ctgacgatca ctctctgttg aagaaacagg
     4741 caggtccgga acggacgtgg gaatctgtcc tgaggatgca ggttgacgcg tgccgagaac
     4801 cgtcagaacc ttagcaggga gagtggcttc ttcccactgg tcagtctcaa tgttccacca
     4861 gggaacatga atcgcgggca acgtcactgt acccggctgc gtgggcacga acacatattt
     4921 ttcctcccgg gaccccacaa tccatttccc gtcaaaatct gtctgggttt ttgccttgtc
     4981 gggatagatc ttcacaatgg gttgtggggg cacggtcact tcaggcaatt gttctccccg
     5041 ctgacctatc gcacgcatca cgatcgttcg agtgggggaa tcccctatct gaagtgtatc
     5101 ggtatcaccc gaccatgttt ctgtgagagt gagatttttc gccggcagcc aaaatcgccc
     5161 atttaaatct gatggaaagt ttttgacgtt gatcgcaatt ttcggacttc gcctatgaat
     5221 cgaacgtgag gttgtgaaag gatcacgccc cagactcccg ggccgattgc caaataattc
     5281 ctcaaaaagc gttccacggg ctcgaccttc ctgcacggtt ccggaaaaga ggatcggagg
     5341 aatcacctgc ttcccgcttt gttgtggtgt aattacataa cgacgttcgg tgatatgata
     5401 gcgtcgcccg tttcgggacg tctcgtacga gacgtccttg cccacgcgtt caacaattcc
     5461 ccaatccatt tcgggttcat ctaaacggcc ttcctgcacg gatatggcgc tgactaacct
     5521 cagcgtcaga aggacttgcc cctgtagata ggaaacatgt gagtccacat ctgcttcgag
     5581 aaaaatatcc tggctttcgt tggcctctct ggtgtgattc ggctcattga cctccagttg
     5641 aatggggccg gtctgatgag cgccaacgtg gataggagga atggtcacgg ctcctgttcg
     5701 ctttggcgca agcgtcgcaa tccatcggac caatgaagtg gttcgcatat tgatgatcga
     5761 agtttgttga ctttgggtag tgcccaatac ctcgaagtct gcgtctaaaa tatgccactc
     5821 gggggattca cccgaagacg tctgatccgt ctccacgatg agctggaagg tctcatctgc
     5881 catgaccgga ttccgatcca cataggccct gactgattgg gccgaaacag gggtagcaag
     5941 ccccataagt ccaaggcaga ccatcatcac ccatatccat ctcaaaattc tcaccagcgg
     6001 ttaccttgag aaatggtgga ctttccagcc tctacacggc gttgatgctc caaaaggaac
     6061 tttcgccgta acaatcctcc gggatcatca gggatacggc gaagccattg ctggaggacc
     6121 tgttcagact tctctatttc cggtgagtgg gcagcgtccc ccgcagaaga tacttctgaa
     6181 tgttccacct gatcttccga cttttcctcc attgtagttg gtgtggaccg cggtttctta
     6241 gattgttcgg ctttcaaagg gtccagccct tgcgcctctc tctcaatatt ttcttcagaa
     6301 gatttctgat ctccattcga ctgattagag gtccgggcag aatccgaccc ggaccctgcc
     6361 atctgggctg cctcttcttg tttctctttt tgagagtcag ctgttttcga atcggattgt
     6421 ttatgggtcg ggtctgaagg attctctgat ttttctcctt ctcctcctgc ctcattccca
     6481 ttattattct gtttggcaga ggagccatcc gattgttggg gtgattcttc cgcctgttcc
     6541 gcagatgatg attcatccaa gagttgtttg atgatttcga gattatgttg ggcgtccgca
     6601 tgatctggct gctgagtgag ggctgtttga tacgaggcca gggcttcttc atagcgaccg
     6661 agattagcca aagcattgcc tcgattgtaa tgaccctcag gggtatccat tatggaaaag
     6721 gcttcttctg cttcctgata tttccctgcc cgataatggg ctatgccctt ccagccgggg
     6781 tcttgaaaca aggctgccgc ttcttccgga gcgtcacgct ctaatgcctg aataccctgt
     6841 tgatcgggac gaacccagag attctcccag gaaaaggcct cgcttacctg aggaataagc
     6901 aggaaggcca gcagcattcc taaccatccc ggtcgaaatg cgggcaatgc caaaatcaga
     6961 agcagaagga cgagccaggg tccttcctct cgccacagat cggttgttcg ctgttcagat
     7021 gttgaagagg atagcgccct cgttggcaca atggaaggaa agacccgatt gatatcatgg
     7081 tcgtcagccg tcaccgtggc ataatgacca cctcccgctc gggcgatggc ttgaagagac
     7141 ggaatatcca atttgggaat aaccaccgtt ccctcctgat ctttgaggaa tccgcctgtc
     7201 tccggaatag gagccccatc gaccgtccca ataccaagca ccgacacacg taccccttga
     7261 cggtgtaact cgcctaccac cttcaatgtg gcaggatctc cctcgccgtc cgttagcaat
     7321 agcacatcac cgcgcgcctg cccgacgtgc tgaaggagtt tatgggcctt ctgcaaggcg
     7381 agatcggtac ggctaccctg gagcggcatc agttccgtct cgagagattg cacaagggtc
     7441 actatggtgt tggcatcatt ggttaatggc gagacgacga acgattcacc ggcatagact
     7501 acaagagcgg tttgtccttc tttccgcgcc ttaagcaaat caagaatctt gtgtttggct
     7561 cgcgtcaacc gtgaaggagt cacatcctgc gcatccattg aacgggataa atccaagacc
     7621 atcaccaacg ccgattccgc ccggaacacg ggctggggta attgggacca caccggccca
     7681 gccagtgcac agaccgccac aatccaacct gatccgagaa gcacgagagg catgcgacgc
     7741 gtagctccct ccgttcccac caacaaatgc gggagtagat gggcgtcaca cacagattcc
     7801 catatctgat ccgttgtccc cttcctggcg acgagtagaa agagtccgaa taagggaagt
     7861 aatccccata accattccgg tcgtagaaaa tgaaacattg ccatcttatc gtccggctcc
     7921 tgcttgtgcg gagatcgctt ccgccctcca tctcgatcgg agaacccaat ttatattcca
     7981 gatgaaagac agggccatta cacaggaaag ggccaacgcg aatcctaacg gccatatata
     8041 taattcggtc gtcggtcgaa acagttctgt ttccgtagcc gccggttcaa gacgatctaa
     8101 ttcttcatac accctcgtaa gtccctcggt gtctttggca cgcaagtaca accctccggt
     8161 acgttgggca aggtggcgaa gggtatcttc atccaaatcg cgagaaggat tcaccgtctg
     8221 tgtgccgaaa aatgattcaa tctccattcg atccgcgcca actccgatgg catagattcg
     8281 aataccttgc tcctcagcca aggccgccgc ttgtgtgggc gagacttctc ccgcggtatt
     8341 ggctccatca gttaatagca ctaaaacccg gcttcccgcg ggttgttctt tgagccgttt
     8401 tacagcaagg ccgatcgcat cgccaatggc ggtttcctgt cccgccaagc caatttctgt
     8461 ttccgtcaac atggccttga ccgtatcccg atcaaatgtc aaaggtgtct gcacgtaggc
     8521 ctgggaacca aatacaatca accctatacg atcgccggtt cggccagcga tgaactcccc
     8581 ggctgcggct ttcaccacct ccaagcgggt gacttcctgt ccttttaccg aaaaatcccg
     8641 aatcttcatg ctgccggaga catcgactgc caacatcaga tcacgtccac tggttggcaa
     8701 gccgacaggt tcgccactcc actgtggacg ggcagcagca agaaccagag cggcccagat
     8761 gaatatcccc gcccaaaaca gcgttgaatg ccttgaacga gtcggcccaa tcaactggtt
     8821 tgactgaagg gccataatgt cgtcgaaatg cggcaccttc atagcccggc cggatgaaat
     8881 ggagacaggg gttgcccaac gacgcaccat ccagggacac agaataagcg ccaacaccca
     8941 cggccaggcc aacgttatca tgccgtgccc ttccctgtgg gtggagtcac ctgctgaatc
     9001 cactgttcga ccaaaggcac cagttcggca gccgacacgg ccgcctgctg ttggtagggc
     9061 cctgaactta acagatggcc aaccccgtca gtgaattgat tggttcgacc cgaccggtcc
     9121 aaaaactgca gccaggagat tcctactagc cctgcgacct cgtttcgagg gaaggtggct
     9181 atggcatagc gacggatcat gactgaaagg cgttgaatca accattgatc atctcgatgg
     9241 gtatcatagt gctgtttgat cacacgtagt tcattcatcg caagtcgaca cgactgtttc
     9301 cgacgtcgat tcctcaaaat ccacaggccc aggacgactc ccatcatgac caaaccgaag
     9361 atgatccacc aacccggagc cggaggccac agagaaatcg gaggtggcag atgcacatcc
     9421 cggagctctt gtaagggaga acttgctgtc ggcatgttag cccattcccg tcatgccata
     9481 ctgtgatggg cacttcggcg atgtccaaga tcctgtaacc caccccgcaa ttggaaaaga
     9541 atatcgtcgt gggttcccaa tggaatgaat ccgattccac gagtcaggca cagagttcgg
     9601 acatcttcaa aacgttccct aaagcaggcg gaataggatt ggattgtgtt ggcagatcct
     9661 gtattcaata ttcccatgcg tgtaccgtcc gtcacaggat attggccggc aggtgggggc
     9721 tcttgttcca attggtcata catgaagatc gccaccacat cctgatgccc gcccaatcgg
     9781 atgagggtct gtttggcctg atgctcccaa tcacgaaaat cacttaagag aaaaatgagg
     9841 cttccgggct tcgcggttgc ctggactcga tttaaggcca actgaaagga tgaggtaatg
     9901 ggactttggc gctcgatcat ctgtgacggg ttggcactat cttgggccag gatattgaga
     9961 agttgtagca ctccggtcct tcctcccctg ggtcggagtt ccacatggtc cacatctgaa
    10021 aaaaccaccc ccccaacccg atctcctcgc tcttgcgatg cccatcccag taatgccgcc
    10081 gcatgggctg caatgaccga cttaaaggcc accttggtcc caaatcgcat tccccttcta
    10141 ttatccacta cgatgaagac aggtcgctcc cgttcttcgc gaaagacctt gaggtagggt
    10201 tcatgagttc gcgccatcac ccgccaatcc atgaggcgta tatcatcacc cggttgatac
    10261 cggcgagatt cctcaaaatc catgcctctg cctttaaacg gagatcgctc tccgccagcc
    10321 aaaagggtat gaacccgctt ccgggtcttg attcccaaaa cccctgtttg gtgacgcatg
    10381 ttgatgagat cggcaagctg aacttctact ccctgaaatc ctgtaggacg gttcagccgg
    10441 aattctggtg gggttgtctg tttacggaac ggccacatga tttagaattt ctgagatcac
    10501 ctgatcggtc ctgatgccct ccgcttccgc ttcaaaggtc agtaagaccc ggtggcgcaa
    10561 cacatcatgg gccacggctt gtatgtcttc cggggagaca taatcccgtc cttccagcca
    10621 ggcatgcgcc ctggcacacc gctccattcc aatggttgcc cgagggctgg ctccaaaccg
    10681 gatccatcgc ctcagcttgg agctataggg tccaggctca cgtgtggcca tgactaattg
    10741 gacgatatat tcttcgagtg cgggcgcaac atatatatcc cacatctgtc gtcgagccgc
    10801 cacaatcacc ttttgaggaa cgggcgtggg ggccgtcgtt acctccccag gaattgccct
    10861 ggcttgtcga cgaacaagtt cgacaatctt ccgctctcct tccacatcgg ggtattgcac
    10921 ccggacatag agtaggaaac gatctaattg cgcttcaggc aaaggatacg ttccttcctg
    10981 ctcaatg
//
LOCUS       DATIAL010000252         3271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1121044, whole genome shotgun sequence.
ACCESSION   DATIAL010000252 DATIAL010000000
VERSION     DATIAL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121044"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VLA60_07820"
     CDS             <1..909
                     /locus_tag="VLA60_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF09305.1"
                     /translation="EDLSQRTSMQASALEETSASMEEMTSTIKQNADNAKQANQLAVT
                     AREVAEKGGMVTDKAVEAMGEINKSSKKIADIINVIDEIAFQTNLLALNAAVEAARAG
                     EQGRGFAVVASEVRNLAQRSATAAKEIKTLINESVQKVGDGSELVNRSGQTLAEIVNS
                     VKRVTDIIAEISAASQEQASGIDQVNKAVMQMDQSTQQNAALVEEATSASQSMKQQAA
                     ELLQQVAFFKSDADTRGPSSERQPRMMTSQVAKTSRKGNLSPVSQSAQALKSPSSVAP
                     KPQPASVGSSNGHDRRQREEDFFEEF"
     gene            1035..2834
                     /locus_tag="VLA60_07825"
     CDS             1035..2834
                     /locus_tag="VLA60_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF09306.1"
                     /translation="MVKRWKALGIGPKVVTTIMVVFAGLLAGGAYMIGQQLQADLERV
                     LADQALIVQKQIEVTRAYVTKNFVVKAKAAGMKTGIDHSQPDTIPFPATFTRETSEQL
                     SHDGVYNARVISQTPLNAMNGPQDSFETDALVALASGQKDFSRNEMVNGKMMFRRATP
                     DLANVQACVGCHEGKKVGDMLGAVSVQIPMDSPMAQLQNNLQNMYLGIFWVGVLMVGL
                     LYLMIAKLVVKPMKGLESMAERAREGDLTARATVISLDEIGRTSESFNAMFDRVSEVI
                     GSVKTAVSGITTGTSEINAGTSDLSERTSAQAGALEETSASMEEMTSTIKQNADNAKQ
                     ANQLAVAAREVAEKGGMVTEKAVEAMDEINRSSKKIADIINVIDEIAFQTNLLALNAA
                     VEAARAGEQGRGFAVVASEVRNLAQRSATAAKEIKSLINESVQKVGDGSELVNRSGQT
                     LEEIVHSVKRVTDIIAEISAASQEQAAGIDQVNKAVMQMDQGTQQNAALVEQATSASQ
                     SMAQQAQDLLAQVEFFKVQEGSKETGGMPKAASPVQLQFKRSRGSQASQTVSLAQTGE
                     QKSSPKPQEISVGAGRSGSNGHKPSEEDFFEEF"
     gene            3070..>3271
                     /locus_tag="VLA60_07830"
     CDS             3070..>3271
                     /locus_tag="VLA60_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09307.1"
                     /translation="MRRFTVFSLMFIMLGLGFALVVPIGQAADAAKEMAAKYILATSK
                     AARTVYVKGIMADATKGGMKPNE"
BASE COUNT          902 a          702 c          933 g          734 t
ORIGIN      
        1 gaagatctct cccaacgaac ctccatgcaa gccagtgcgc ttgaagagac gagtgcgtca
       61 atggaagaaa tgacctccac gattaagcaa aatgccgata atgccaagca ggccaatcaa
      121 ttggcggtca ccgcccgcga agtggccgaa aaaggcggga tggtcactga caaagccgtt
      181 gaggccatgg gagagatcaa caagagcagt aagaagatcg cggatattat caacgtgatc
      241 gatgaaatcg cctttcagac aaatctgttg gcgttaaatg cggctgtaga agcggcacga
      301 gccggggaac aggggagggg ttttgcggta gtggcctcag aagttcgcaa tttggcgcaa
      361 cgatcagcga cggcggccaa ggaaatcaag acgctaatca acgaatcggt gcaaaaagtt
      421 ggcgatggca gtgaattggt gaatcgctct ggccagactc ttgcggaaat cgtgaattcg
      481 gtgaaacggg tgacggacat aatcgcagaa atcagtgccg cttcccagga gcaggcgtcg
      541 gggattgacc aggtgaataa ggcggtgatg cagatggatc agagcacgca acaaaatgcg
      601 gctttggttg aagaagccac ctcggcgtcg caatccatga aacagcaagc cgccgagctg
      661 ttacaacagg tcgccttttt taaatcagat gcagacacac gaggaccgtc gtcagaacgc
      721 caaccccgta tgatgacgag tcaggtggcc aagacaagcc gaaaagggaa tctttctccg
      781 gttagccagt cagctcaagc gcttaaatcg ccatcatctg tggcgcccaa gccgcaaccc
      841 gccagtgtag gcagttccaa tggtcatgat cgtcgtcaac gcgaggaaga tttttttgaa
      901 gagttttaat gggaaaatga ataggtcacg cctgcggggg accgggggta ttatacttgc
      961 ccgatagtcc tctcttaaat tttcccgtat caggaattat tcatttacga caaagaaagg
     1021 agccaggagg cattatggtc aaaagatgga aggcgttagg aattggtcct aaggtggtga
     1081 caaccatcat ggtggtcttt gccggtttgc tggctggagg tgcatatatg atcggtcagc
     1141 aactacaggc ggatttggag cgtgtcctgg cggatcaggc tctgattgtt caaaaacaaa
     1201 ttgaagtgac gcgagcctat gtcaccaaaa attttgtggt caaagcgaaa gccgcgggga
     1261 tgaaaacggg gattgaccac agccagcctg acaccattcc gtttccggcg acgttcacaa
     1321 gggaaacgtc cgaacagttg tctcatgacg gagtctataa tgccagagtg atcagccaaa
     1381 cgccgctcaa tgcgatgaat ggacctcaag attcttttga aacggatgcc ttagttgccc
     1441 tggcttccgg tcaaaaggac ttctcccgaa atgaaatggt gaatggcaaa atgatgtttc
     1501 gccgggcaac tccggattta gcaaatgtgc aggcgtgtgt gggttgtcat gaaggaaaaa
     1561 aagtgggcga tatgttagga gcggtatccg tgcaaattcc gatggattcc cccatggctc
     1621 aattacaaaa caaccttcag aatatgtatc tgggaatttt ctgggtcggt gtgctgatgg
     1681 tgggtttgtt atacctgatg attgcgaagt tggtggttaa gcccatgaag ggtctggaat
     1741 ccatggccga gcgagccaga gaaggtgatc tcacggcccg agcaactgtg ataagtctgg
     1801 acgaaattgg gcgaaccagc gaatcgttta atgcgatgtt tgatcgggtg agtgaagtca
     1861 tagggtcggt taaaacggcc gtgtcaggta ttacgaccgg tacttctgaa attaatgcgg
     1921 gtacctcgga tctttccgag agaacatctg cccaagcggg agcgcttgaa gaaacgagtg
     1981 cctccatgga agaaatgaca tccaccatca aacaaaatgc ggataatgcc aaacaggcca
     2041 atcaattagc tgtcgctgcc agggaagtcg cagaaaaggg tgggatggtt acggagaagg
     2101 ctgtggaagc tatggatgag atcaacagaa gtagcaaaaa aattgcggat attatcaacg
     2161 taattgacga aatcgccttc cagacaaatc tgttagcctt gaatgccgcc gtggaggctg
     2221 ctcgtgcggg tgaacagggg aggggttttg cggtagtggc ttcagaggtg cgaaatttgg
     2281 cgcaacgatc cgccacagct gcgaaggaga ttaaatcgtt gattaacgag tcggtgcaaa
     2341 aagttggtga cggcagtgaa ttagtcaatc gttcaggaca aacgcttgaa gagatcgtgc
     2401 attctgtcaa acgagtgacg gatattattg cagaaatcag tgcggcctcc caggaacagg
     2461 cggcgggaat cgaccaggtg aataaggccg tgatgcagat ggatcagggc acacaacaga
     2521 atgccgctct tgtggaacag gccacatcgg catcccaatc gatggcgcag caagctcagg
     2581 atctcttagc ccaggtcgag ttttttaagg ttcaagaagg atcaaaagaa accggaggca
     2641 tgccgaaggc ggcatcgcct gttcaattac aattcaaacg ttcaagaggc tctcaagcat
     2701 ctcagaccgt atcgttggct cagacgggtg aacaaaaatc atccccgaag cctcaagaga
     2761 tatcggtggg tgccggccgg tcaggttcga acggtcataa accctcggag gaagattttt
     2821 ttgaggaatt ctaggggaat gctcattaat ttggatgggg cgaaggggga gatcatgtta
     2881 acacttcggc acttgctgaa tccctttcta aagttgggcc tatttttatc cgaaaaactt
     2941 atgaggactc tatgagttgg tttatcaaca caatgttggc cttgttatgt tgtaagcctt
     3001 taatttctca taacttggcc ggactcattc gcatgttatg tccctacact ttaacggagg
     3061 aggattggta tgagaagatt tactgtgttt tcgctgatgt ttatcatgtt aggtcttggc
     3121 ttcgctttgg tggtgccgat cggtcaagcc gccgacgcgg ccaaagaaat ggcggccaaa
     3181 tacatcttgg ccacctctaa ggctgccaga actgtgtatg tcaaggggat tatggccgat
     3241 gcgaccaaag gcggaatgaa acccaatgaa g
//
LOCUS       DATIAL010000253         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_476169, whole genome shotgun sequence.
ACCESSION   DATIAL010000253 DATIAL010000000
VERSION     DATIAL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476169"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="VLA60_07835"
     CDS             complement(<1..1996)
                     /locus_tag="VLA60_07835"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09308.1"
                     /translation="MNFYAFSGLLNGLAATASGLIVYAKNPHSSKHQAYALYCLAAAI
                     WGYGYCAWHLSSTHDLALLFVRLLMAGAIFLPPAYLWHVLTLLDDVEANRWVIRLGWG
                     ASLIYFGANFTTYFVADVHHVGDFPFWPQPGPFFHVYLLGFGLSTIYGTWLLYRGART
                     RTGPERSRFFLLTIGSVIAYLGGMTTFPLWYGIEIPPNGTILLTVYTSVVAYTLLRYR
                     LMDLGTAVERSITGGLLLALVAFPAYPILLLGQSLYFGQINIAYSFVELSVLLILVLG
                     ASSLKREAKTLITKSLFKERYNRHETVVHFSKSLLSILELKDLTATIVESICPTLGLQ
                     WGVLYLRSSSSQDYRPVSSFGKSTTDLPVLHLHKTPTLLEMWRQGTSCFVIHELELST
                     INSDSEAVIAQLAHIGTEVCLPLVNKTRLLGFCVFGPNTKSGGGYTTQDLDLLTTLSQ
                     EASVALDNALLYEELKRSQSLVRRTDRLRSLETMAGGLAHEIRNPLTSIKAFVDLAPE
                     RKDDEQFLVRFSKVVKEDVFRIERLTREILDYAKPMEPFLKEEDLNDIVESCLYTLRI
                     RPSHELIVVETDLAHGLPKVFADRQQLKQVFLNLLFNAVEAMLPEGGVLTVRTRKIGS
                     GVAQGWVQVDIHDTGKGINPEDIEHIFDPFFTTKHLSQEHE"
     gene            complement(2244..>2901)
                     /locus_tag="VLA60_07840"
     CDS             complement(2244..>2901)
                     /locus_tag="VLA60_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF09309.1"
                     /translation="SGISYRGKSIQYVGIDIVPDAMKRANKAMFAADTEWQHGMASPT
                     PFVTKAWAQVDLGQPLPFPDNRFDRIVSNLVIGYVQDPGATLRELYRVLAPGGRIVIS
                     NLKPNGDFSGIYQNLVSSAALPQQREEARDLLNNYGKIRQAEKEGQFCFYDQTQWEAI
                     VATLGCTNAGIFSTFSEQAYLVVLDKPIAYTQAQRPMIQNAELYPTRNQLPGLLKQVA
                     "
BASE COUNT          823 a          691 c          678 g          709 t
ORIGIN      
        1 cctcatgttc ctgactcaag tgttttgtgg tgaaaaatgg atcaaatata tgctcaatgt
       61 cttccggatt tatccctttc ccagtatcgt gaatatccac ctgcacccaa ccctgcgcca
      121 cacctgaacc aatctttcga gtccgcaccg ttaacacccc accctctggg agcatggctt
      181 ccacggcatt aaacaacaaa ttgaggaaca cctgcttcaa ttgctgccga tccgcaaata
      241 cctttggcaa tccatgagcc aaatctgttt cgacgacaat taattcatga gagggtcgaa
      301 ttctgagggt atacagacag gattccacaa tatcattgag atcttcttcc ttgagaaacg
      361 gttccatcgg tttggcataa tccaagatct ctcgagtgag ccgctcaatt cgaaagacat
      421 cctctttgac gaccttactg aatcgaacca gaaactgttc atcatccttt cgttcaggtg
      481 ccagatccac aaaggcttta atcgacgtca agggatttct aatttcgtga gccaatccgc
      541 cggccatggt ctccagagaa cgaaggcgat cagttcgacg cactaacgat tgggagcgct
      601 tgagctcttc atagagcaag gcattgtcta acgccacgga cgcttcttgg gataaggttg
      661 ttaataaatc caaatcctgg gttgtatatc cgcctccact tttagtattt ggtccaaaca
      721 cacaaaaacc taagagccgt gttttgttaa ccaagggcaa acaaacttct gttccaatat
      781 gagcgagctg cgcgatgacg gcttcagagt cggagttgat ggtagaaagt tccaattcat
      841 ggatgacaaa acaggacgtg ccttgtctcc acatttctag taacgtaggg gttttatgca
      901 aatggaggac tggaagatct gtggtggatt ttccaaagct ggataccggg cgataatcct
      961 gactactgga actccgtaaa tataagacgc cccattgcaa tcctaatgta ggacaaatgg
     1021 attccacgat agtcgccgtc aaatctttta attccaatat agagaggagg gacttggaga
     1081 aatgtactac cgtttcatgt cggttatatc gttctttgaa cagtgatttc gtaattaatg
     1141 tttttgcttc ccgcttcaag gaggacgctc caagcaccaa gatgagtaac acgctcagtt
     1201 ccacaaaaga ataggcgata ttaatttgcc caaaatacag actttggcca aggaggagga
     1261 tcggataggc agggaaagcc acgagtgcta acaggaggcc tcccgtaatg cttcgttcaa
     1321 cagccgtccc gagatccatg agtctgtatc gtagaagcgt ataggcgacg accgacgtat
     1381 agaccgtcag taggattgtg ccgttcggtg ggatttcaat tccataccag agcggaaaag
     1441 tcgtcatccc tcccaagtat gcaatgactg atccgatggt gagaaggaaa aatcgtgaac
     1501 gttcaggacc ggttctggtc cgagctcccc gataaagcaa ccacgttcca taaatagtcg
     1561 ataggccaaa tcccagaaga tagacatgaa aaaatggacc gggttgcggc caaaaaggaa
     1621 aatcaccgac atgatggaca tcagcaacga aataggtggt aaaattcgcg ccgaaataaa
     1681 taagacttgc tccccatcca agacgaataa cccatctgtt ggcttccaca tcatccaata
     1741 aggtcaggac atgccacaga taggctggcg gaagaaaaat ggctccggcc attaacaatc
     1801 gaacaaagag aagggcgaga tcgtgagttg aggaaaggtg ccatgcgcaa tacccatagc
     1861 cccaaatggc ggcagccaaa caatacaggg cataggcttg atgtttggag ctgtgaggat
     1921 ttttggcata gacaataagg ccggaggctg tggcggccag accattcaga agtccgctga
     1981 aggcgtaaaa attcacgtct aaaacaatga cagaagagag atcacactag atcattggct
     2041 ctgacatctt tgaaggccta ttggacaata aatgcaggca gaagtcaaaa ggggtaagtc
     2101 catgaatgcc ggtggatacc tactcttgtg cccccgtgaa cgacaccctt ctgtgcaatc
     2161 catgctcagt cattgtcata ttgatgttcc attcaggcca tgcggtcctc atggaaaata
     2221 tttatagata gaaaagggcc atcctatgcc acttgcttca gaagaccagg cagctggttc
     2281 cgtgtggggt aaagttctgc attctggatc attggccttt gcgcttgtgt gtaggcaata
     2341 ggtttgtcta aaacaacgag atacgcttgt tctgaaaacg ttgaaaaaat acccgcattc
     2401 gtacagccca aggtggcaac gatcgcttcc cattgggttt gatcataaaa acaaaattgt
     2461 ccctcttttt cggcctgtcg gattttcccg taattattca atagatcccg tgcttcctct
     2521 cgttgttgtg ggagagcagc actactgacc agattctggt aaataccgga aaagtcacca
     2581 ttgggtttta aattggaaat aacgattcgt ccccctggag cgagaacacg atacagttct
     2641 cgcagagtag cgcctggatc ctgcacatat ccaatcacca gattagagac aatccggtcg
     2701 aatcggttgt ccggaaaggg aagcggctga cctaaatcca cctgcgccca tgcctttgtg
     2761 acgaagggtg tcggagaggc catcccatgt tgccattctg tgtcggcggc aaacatcgct
     2821 ttgttagccc gtttcatcgc gtcaggaacg atatcaatcc ccacatactg aatggacttc
     2881 cctcggtacg aaattccact g
//
LOCUS       DATIAL010000254         3467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_799209, whole genome shotgun sequence.
ACCESSION   DATIAL010000254 DATIAL010000000
VERSION     DATIAL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799209"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VLA60_07845"
     CDS             <1..431
                     /locus_tag="VLA60_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HSF09310.1"
                     /translation="DPHNLGAIVRSVEAAGGDGIVIPKHRAVGLTGGVAKASAGALEH
                     VSIARVANTGKFLETCQNRDIRTVALVPEGVESYSEVDLTEPVALVFGSEGEGIRPIV
                     LKKCDQQVRIPMFGRMNSLNLSVAVAVTLFEAVRQRNQKP"
     gene            complement(446..706)
                     /locus_tag="VLA60_07850"
     CDS             complement(446..706)
                     /locus_tag="VLA60_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF09311.1"
                     /translation="MTNQLKPDKLDMPPIKRKRPEALTQREQEILQLIWSGLKNKEIA
                     QQLKISVKTVEAHRANMMKKVRVSNAAQLLNAAIQEGLIQIK"
     gene            832..1605
                     /locus_tag="VLA60_07855"
     CDS             832..1605
                     /locus_tag="VLA60_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HSF09312.1"
                     /translation="MSHWIVWPSVFILGTIIGSFLTVCVHRVPKGQSVVAPRSACPHC
                     GRQISWYDNVPFLSFIWLLGRCRACHAAIPPRYPIIELANGFGYLLVVWRFGLTWPTI
                     VYAGLVSVFLVVAWIDWDHKIIPDVITLPGIVVGFLCASFLLPTGWVNSLIGLLVGGG
                     ILLALAWVSPFLFGKEGIGGGDIKFLAMVGAFLGWQQAILTLMVGSVVGAVVGIMLLA
                     TKVLEKGQYIPFGPYLALGGLIAVVWGTELWHWYFHGLL"
     gene            1683..2144
                     /locus_tag="VLA60_07860"
     CDS             1683..2144
                     /locus_tag="VLA60_07860"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HSF09313.1"
                     /translation="MKRSLLCTRGFTLTELIIVLGIIGVVLMLAQTWLVSQLPHWRLN
                     GAARQVTSDLLGAKMKAVIERNRQRVFFQDNHRYVLLDDDNNNGKSDPGEHLVARDIQ
                     ESYRDVIMTASNNPSFLPRGTASSLASITLSNSAGKRIITVSITGRVKVKS"
     gene            2144..2560
                     /locus_tag="VLA60_07865"
     CDS             2144..2560
                     /locus_tag="VLA60_07865"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSF09314.1"
                     /translation="MNIMMHEGYRLTSCLQDQRGFSLLEVLMALVVVFLALLGFAGYS
                     VVAHTGMTASEKMTRAVTLAQEKLEDVRRDGVPNSLTAAWANTEPYGSIAGASHHQRT
                     LTIQPHFPISGLHTVTVEVRWDHDAHTTTLTTYLTK"
     gene            2575..3183
                     /locus_tag="VLA60_07870"
     CDS             2575..3183
                     /locus_tag="VLA60_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09315.1"
                     /translation="MPHVPIGSPQGYTLLEVVVALGLSLLTVSVVYALYIQELKAQGV
                     REQILDMQQQARVVLDLVTREILMAGYDPRGVNRDADPTNDFEGITYDPQKLSIKADM
                     NGNGLASDANESIVFVYDATTHMLRRNTGGGNQPFGEDIEAFVIDYLDQGGNPTTDSK
                     AIRQVRISVTARTSQHDPQYAKNGGYRTVTLHSRITMRNVQS"
     gene            3180..>3467
                     /locus_tag="VLA60_07875"
     CDS             3180..>3467
                     /locus_tag="VLA60_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HSF09316.1"
                     /translation="MTGFNSQKGIRPYHNSLGGALIAALLLVAISTIMGATILFATST
                     DLQISGNFRRAMATFYAAEAGIAETAVRLGGSSLSNPGYLGDPSPILQANWS"
BASE COUNT          815 a          768 c          951 g          933 t
ORIGIN      
        1 aggatccaca taatctaggc gcgattgtcc gttcggtaga ggcggccggt ggagatggga
       61 ttgtcatacc caagcaccgt gccgtgggtc tgacgggtgg agtggccaaa gcatcggctg
      121 gagctttaga gcatgtctcc attgcccgtg tcgccaatac gggaaagttt cttgaaacat
      181 gtcaaaaccg ggacatcagg actgtagcat tagtcccgga aggagttgaa tcctattcag
      241 aggtagacct cacagaaccc gttgcgctgg tgtttggaag tgaaggagaa gggatccggc
      301 cgattgtcct aaaaaaatgc gatcaacaag tgaggattcc gatgtttgga aggatgaact
      361 cgctgaattt gtcagttgcc gtagcagtga cactttttga ggcagttcgg caacggaatc
      421 agaaaccata agagccatgc ggcaattatt ttatctgaat caggccttcc tgaattgcgg
      481 cattgagcag ttgagcggca ttagaaaccc gaaccttctt catcatattg gcccggtggg
      541 cttcgaccgt tttgacgcta atttttaatt gctgtgcaat ctctttattc ttcaaccctg
      601 accagatgag ttgcaggatt tcttgctcgc gttgagtcag ggcctctggg cgttttcgct
      661 taataggtgg catgtccaat ttatccggct ttagttggtt ggtcatgggt acctcaggca
      721 ttttctctag gcgaattatc taagggagaa tatcaggtca ttttaggatt gtaaaccgaa
      781 agaatacgta aactcactct agtcgaatta actacggaat atgtgacctt tatgtctcac
      841 tggattgttt ggccgagcgt ctttattctg ggaacgatca ttggaagttt cttgacggta
      901 tgtgtgcaca gagtgccaaa gggacaatct gtggtggctc ctcggtccgc atgtcctcat
      961 tgcggcaggc agataagctg gtatgacaat gttccttttt tgagttttat ctggctgctg
     1021 gggaggtgtc gagcttgcca tgcggctatt ccgccaagat atcccattat tgagttggca
     1081 aatggctttg gctatctcct ggtggtctgg cggtttgggc tcacgtggcc cacgatcgtg
     1141 tatgcggggt tggtgtcggt gttccttgtg gtggcttgga ttgattggga ccacaaaatc
     1201 attccagatg tgattacatt acctggaatt gtggttggct ttttgtgcgc atccttcctg
     1261 ctaccgacgg gctgggtcaa ttcgcttatc ggtctgttgg tgggaggggg gattttacta
     1321 gctctcgcgt gggtcagtcc cttcttgttc gggaaagaag gcattggggg tggtgatatt
     1381 aaatttttgg ccatggtggg agcctttttg gggtggcagc aggccatact taccctgatg
     1441 gtgggatcag tggtgggagc ggttgtcggg ataatgttac ttgccacgaa ggtccttgaa
     1501 aaaggccaat acattccttt cggaccttat ttggcacttg ggggcctgat cgcggtggta
     1561 tggggaacgg aattatggca ctggtatttt catggcttgt tataagtacc caacgaattc
     1621 agcaaggcag gtgcgatcac acggagtcgg ttcaagacgt cactgtaaca gggataagac
     1681 gattgaagag atctcttttg tgtaccagag gttttaccct cactgaatta atcatcgtgc
     1741 ttggcattat cggggtagtc ttgatgttag ctcagacctg gttggtatcc cagttacctc
     1801 attggcgatt gaatggagca gccagacagg tgacctctga ccttttgggg gcaaaaatga
     1861 aggccgtgat tgagcggaat aggcaacgag tcttctttca ggataatcat cggtatgtgt
     1921 tattggatga tgataataat aatggaaagt ccgacccggg cgaacatctt gtagctcgcg
     1981 atattcagga gagttatcga gatgtcataa tgacggcatc caacaatccc tcctttctgc
     2041 caagaggaac ggcgtccagc ttagcttcta ttaccttgtc gaattcagcc ggaaaacgga
     2101 ttattacggt tagcataacc ggacgggtaa aagtgaagtc ttaatgaaca tcatgatgca
     2161 tgagggatat cgactgacga gttgcctgca agatcaacga gggttctctt tgttagaagt
     2221 gctaatggcc ctggtggtgg ttttcctggc gctccttgga tttgcaggct attccgttgt
     2281 cgcccatacc ggcatgaccg cgagtgaaaa aatgaccaga gccgtcaccc tcgcccagga
     2341 aaaattggaa gacgtgcgcc gggatggagt gcccaattcg ttaaccgctg catgggctaa
     2401 cacggaaccc tatggctcca ttgccggcgc gagtcatcat caacgaacgc tcactattca
     2461 accgcatttt ccaatatccg ggttacacac cgttacagtg gaagtgcggt gggatcacga
     2521 tgcgcatacc accacactta ccacttatct cacaaagtaa aagtgaatac gcggatgcca
     2581 catgttccga ttgggtcccc tcaggggtat acactccttg aggtcgtggt ggccctgggc
     2641 ctgagtctcc tgacagtgag tgtggtctat gccctgtaca tccaagaact caaagctcag
     2701 ggtgtgcgag agcaaatctt ggacatgcag caacaggctc gcgtggtgtt ggatttggtg
     2761 acccgggaaa tcctgatggc cggttatgat cctcgcggag tgaatcgtga tgccgatcct
     2821 actaatgact ttgaggggat tacgtatgat ccacaaaagc tctctatcaa ggcggatatg
     2881 aatggtaatg gcctcgccag cgatgccaat gaatcgattg tctttgtcta tgatgccacg
     2941 acccacatgc ttcggcgtaa taccggtgga ggaaatcaac catttggtga ggacattgaa
     3001 gcgtttgtca tagactatct tgatcagggt gggaacccga ccacagattc caaagctatt
     3061 cgacaagtga gaatttcggt gacagcccga acgtcacagc atgatccaca gtatgccaaa
     3121 aacggtggat atcgaacggt gactctccat tcccgaatca cgatgagaaa tgtccaatca
     3181 tgaccggatt caatagccag aaaggaataa ggccttatca caattccttg ggtggagcgc
     3241 tcattgcagc cttgttgttg gtcgctattt ccacaattat gggtgcgacg attctgtttg
     3301 ccacgtccac cgatctgcaa atcagtggaa atttccgaag ggcgatggct acgttctatg
     3361 ccgcagaggc gggcatcgca gaaactgccg ttcgtttagg tggatcttcc ttgtcaaacc
     3421 cagggtatct cggcgatcct tctcccatcc tgcaagcaaa ttggtcc
//
LOCUS       DATIAL010000255         2690 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1122042, whole genome shotgun sequence.
ACCESSION   DATIAL010000255 DATIAL010000000
VERSION     DATIAL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122042"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(591..1478)
                     /locus_tag="VLA60_07880"
     CDS             complement(591..1478)
                     /locus_tag="VLA60_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HSF09317.1"
                     /translation="MAKSKRQTQSVGPETARKPSNAPSSLFSGSKALLDLLIIILAGA
                     GLMLTAYLTYTASFEVHPAFCSEGSGCDIVQSSRWATFLGIPMALWGFVTYVVIAGLA
                     WSSRGKSGSGSVLIYVAMGGFAVSAYLTIVSVVEIEATCQYCLTSFAIITTILGLALA
                     RRPPDWTKSLKEAVVIGLVIVGGLHLHYSGVFDAAAGPEDPQLQALAIHLNDTGAKFY
                     GAYWCPRCQEQKAEFLSSAKRLPYVECSSGGRGSALTAPCVKADIKSYPTWIIGERRL
                     TGLKTPQELARASGFNWQE"
     gene            complement(1920..2471)
                     /locus_tag="VLA60_07885"
     CDS             complement(1920..2471)
                     /locus_tag="VLA60_07885"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HSF09318.1"
                     /translation="MEKMSFTFLALVVIGCASTTMPIAAQSGSTPKDGELVFPADYKS
                     YAAFLHGIQKSDAVRDLYINLTGASAEAGQPFANGSILVMEIYNAKKNAEGTLEKGAD
                     GNLVKADLAKVFVMQKDAGWGKHAPENLKNGDWISSAFKANGDRLDVDYTTCRGCHLP
                     LGASKDFVHRYDEYFEKRIQAAH"
BASE COUNT          652 a          645 c          730 g          663 t
ORIGIN      
        1 acagtttttt tgttaaatga aaaaactaga accattcaac caagttttct cgaaaagtca
       61 aagagtgatc gacttcagac ttatttctca agcccaagcc aacggggaag tagaggaacc
      121 cggcgaatga gcagtttttc aataggccgt atggatgcca ggaataatga ttgttcaaga
      181 gttcgagcat catggagagg aaaaagaaca tctatttatg aattttagaa taaaaagtca
      241 gggtgaaggc caaggaggtt gcaagtccaa atgccttcac cctgaagtgg agcggggtgg
      301 gtcagggagg agtcacgccg ctccgggttc cgctggaaga gtgcaatgaa tttttggcaa
      361 caaaaacaga tatacccgac ttccgacttt tgctctacgg acgtgtgccg atgcttgcta
      421 cattgaggcg gttcaagggc cgcatccttg gagatcatgg ttgaatgact ttcctgcgtg
      481 gtgcagtttt ttaaccctct accggagttc tggaaggagt ggaagcagga agggcacgaa
      541 taaaagaaac caagaggatt ctgagtttct gaaaggcgcg gctgggcctt ctattcctgc
      601 cagttgaacc cggaggctcg tgccaattct tgtggggtct tgaggccggt taaccggcgt
      661 tcaccgataa tccacgtggg gtagctttta atgtcagcct tgacgcaggg agcggtgaga
      721 gcgcttcctc gtcctccaga actacattcc acgtaaggaa gccgtttggc ggacgaaaga
      781 aattcggctt tttgttcctg gcagcgtggg caccaatagg caccgtaaaa tttggcgccg
      841 gtatcattga gatgaatggc taaggcttga agttggggat cttccgggcc ggcggctgcg
      901 tcgaacaccc cgctataatg caaatgaagc ccgccgacga tgacaaggcc gatcacgact
      961 gcctctttca gtgatttcgt ccaatcggga ggccgtcgag cgagagccag accaaggatt
     1021 gtcgtgatga tggcaaatga agtcaggcaa tattgacagg tggcttcgat ctctacgacc
     1081 gagacgatag tcagataggc gctgacggcg aatccaccca tagccacgta aatcaacacc
     1141 gatccgctac cggattttcc acggctggac caggcgagcc ccgcaatcac cacataggtc
     1201 acgaagcccc acaaggccat ggggattcct agaaaggtgg cccatcggct gctttgcaca
     1261 atgtcgcagc ccgagccctc gctgcaaaat gccgggtgaa cctcgaacga ggcagtatag
     1321 gtcaggtaag cggttaacat tagcccggca ccggccagaa taatgatgag aaggtcgagg
     1381 agggctttcg aacctgagaa cagggaagac ggagcgttag atggctttct tgctgtttca
     1441 gggccgactg attgtgtctg tcgtttggat tttgccatgg cgtccctcgt gaacgagtgt
     1501 atcatgggct tattgccatt gaaatcccga caatctatca aatactcctg accgtagagg
     1561 agacttttgc caaggccatg tcaggccggt atttcgcagg atgggttgct gatcacaggg
     1621 gtaacacttt aaaaggcatg ggttgtttca agttctgacg taaggtagag actttgtggt
     1681 cttttaggta tcgtgaggcc ctacgccgaa actatgtgcc gcgaaattta tttggccctg
     1741 ttgagctgag ttgaggaagg aaaaggtggg gtgcgcatag aagtagggtg aaggcctagg
     1801 aggctgcaga tgcgcaaacc accttcaccc tactcgtcca aggttggagg ccggtccgtc
     1861 cctggatgtt ctacgccaat tcgttctgtg tctaacctgg aagcgatgaa caggtctggt
     1921 taatgagccg cctgtattcg tttctcaaaa tattcatcat accggtgcac aaagtctttg
     1981 gatgctccca aaggtaaatg acacccccgg caggttgtgt aatcgacatc caatcggtcg
     2041 ccattcgctt tgaacgctga ggagatccaa tccccattct tcaggttttc cggggcgtgt
     2101 ttcccccatc ccgcatcttt ttgcatgaca aagaccttgg ctaaatccgc cttcaccaga
     2161 tttccatccg cccctttttc gagtgtccct tcggcatttt tcttggcgtt gtatatttcc
     2221 atcacgagaa tgctgccatt ggcaaatggt tggcccgctt ctgcgcttgc ccctgtgagg
     2281 ttgatataga ggtcgcggac ggcatccgat ttttgaattc cgtggagaaa ggcggcatag
     2341 gatttgtaat cggccggaaa gactaattcg ccgtccttcg gcgtggagcc ggattgggct
     2401 gcaataggca tggtggtgga tgcacaccct ataaccacca aagccagaaa cgtgaaactc
     2461 attttctcca tatttctccc cctcctttgt tgatatctcg tccgattgac ggaaccgaca
     2521 cattgtgaca agtcacattc agaacgttga cacggtggct ccagcatacg atggacccta
     2581 tagtccgtct acagatgggt actgcctgtt gctacagcgc gatggtccgt attgtatccc
     2641 gccctcattt ccagaatttt ttgggttagg gatatttcga aggcacataa
//
LOCUS       DATIAL010000256         2702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1553221, whole genome shotgun sequence.
ACCESSION   DATIAL010000256 DATIAL010000000
VERSION     DATIAL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553221"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VLA60_07890"
     CDS             <1..1207
                     /locus_tag="VLA60_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09319.1"
                     /translation="IHRKNIETQLQSLTESLERRVDERTQDLLQHQHRLRQLSLDLIL
                     TEQRERRRIATELHDYLGQLLVVGKIKISQLQQADLSNQQAPFVREVEESLDEALQYT
                     RDLIPQISPPILYEFGFMAAIRWLAEKMVRYDLHVTVTSHVEENQLNLSESVSIILYH
                     VIRELLLNVMKHAQTQEASILIKQVSSEFFGIEVADRGCGFDLSSTRETHPHFEKFGL
                     LNVQERVEGLGGECEVLSNVGEGTRVLIKMPFSLQDAELEPIQSTLPHMAVQVPKSGK
                     SEESIRVVLADDHPIFREGLGTMLNACPDIQVVGEAENGEQAAELVQALHPDVVVMDI
                     NMPVMNGIEATRLIKASHPGIYIIGLSMHGDQLVTNNFAEAGGDEYVSKGDSFNSFAE
                     VIRSSQKNR"
     gene            1850..2155
                     /locus_tag="VLA60_07895"
     CDS             1850..2155
                     /locus_tag="VLA60_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09320.1"
                     /translation="MQTFTLSAMRTEHPDVFPQLIHQMQQPHCARCGGTMVEETYMDI
                     FSDYEDFQFQAKHCIQCGDVIDPFILLNRLRKHKGETPAVNSLSQPRHRENGLVMTG"
     gene            2218..2598
                     /locus_tag="VLA60_07900"
     CDS             2218..2598
                     /locus_tag="VLA60_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09321.1"
                     /translation="MNGKKCSFYWMCGILLSLIMEGNGIAAEMPKEPSETYLINEDVN
                     IITGLYTREYSLKGDGVVDYKTARQIIFYENNKFWNTVVETVEWPLFYWLDDDRDGVF
                     DQFVDQRVEGNGAYIIPYLPVSEN"
BASE COUNT          688 a          642 c          660 g          712 t
ORIGIN      
        1 catacaccgg aaaaatatcg aaactcaact gcaatctctg accgaatcgc tggaacgccg
       61 cgtggatgag cggacacagg acctccttca gcaccaacac cgacttcgtc aattgtcgtt
      121 agatcttatt ctcacggagc aacgggaacg gcgtcgcatt gccaccgaat tacatgatta
      181 tttggggcag ttgctggttg tcgggaaaat taaaatcagt cagttgcaac aagccgatct
      241 ttcgaatcag caggcaccct tcgtcagaga agtggaagaa tctctggatg aggcgttgca
      301 atacacgcgg gatctcatcc ctcaaatcag tccgcccatt ttgtatgaat tcgggttcat
      361 ggcagccatc cgatggttgg ctgaaaaaat ggtccggtat gatttgcacg tcacggtgac
      421 atcccatgtt gaagaaaatc aattgaatct ttccgaatcc gtctcaatta tcctgtatca
      481 cgtcattcgg gaattattgc tgaatgtgat gaaacatgcc caaacccagg aagcctcaat
      541 tctgatcaaa caagtttcat ccgagttctt tggaatagaa gtggcggatc gcggatgtgg
      601 ttttgatctg tcttcgaccc gtgaaaccca tcctcatttt gaaaaatttg gcctgttaaa
      661 cgtgcaggaa cgtgtggaag gtcttggagg agagtgtgag gtgctttcga acgtcggcga
      721 gggcactcga gtgctgatca agatgccctt ttcccttcaa gacgctgagt tggagcccat
      781 tcagtccacc cttcctcaca tggcggtgca ggttccgaag tccggaaaat cggaggagag
      841 cattcgggtc gtccttgcag atgaccaccc catcttccgt gaaggattgg gcaccatgtt
      901 gaatgcctgt cccgatattc aggtggttgg agaagctgaa aacggcgaac aggcggctga
      961 gcttgttcag gctctccacc ctgatgtggt ggtgatggat atcaatatgc cggtgatgaa
     1021 cggaatagaa gccacccgtc taatcaaagc cagccacccg ggaatctata tcatcggctt
     1081 gtccatgcat ggggatcaac tcgtaacaaa taattttgct gaggccggag gagacgaata
     1141 tgtgtccaaa ggggattcct ttaattcctt tgccgaagtt attcgatcca gtcaaaaaaa
     1201 tcgatgatgt ctcctgaacc ctttacacaa gtcatatagc gcacggtgaa atgttaggag
     1261 taaggagaaa acctccatcc cccctacatg actcatcacg atccccttcg acctcccagt
     1321 tctgcggttg tgatgctgag ctgaaggcga aacatctcgc aaagccgtgg tccggtcgaa
     1381 ccggacacag atccttcccg tctctcagga acgctcagac cgcttaactg aggcttcacg
     1441 atctccttcg gcgttcccct gctgtcggaa ttcaaaattt ttacaatctt tcatgaccga
     1501 agcaggatgt tcaaacggcc tgtcccgtcc tatggttttt tcagctcgag tgcgtaactt
     1561 tccagaaact ttctgcggat tggtttcagg gcgagtcgcg ggaatttcgt tattgtccat
     1621 tccttcacgt tgatgcgcag actggtcctg tctctttctt catgggcaga tgtcatccca
     1681 tgagactaag tccctctttc cctcattcaa aacccccata gttcattcga catttttcaa
     1741 gggatcttca cctctagacc cagggttttg ggtagtaggc gctttcgggg tgggaaacta
     1801 cagtcagacc atgaacatga tttcttacct taagaaagaa ggaggcctta tgcaaacatt
     1861 tactctgtcc gcgatgagaa ccgagcatcc cgatgtgttc cctcaattaa ttcatcaaat
     1921 gcaacaacca cattgtgcac ggtgcggagg aacgatggtc gaggagacat atatggatat
     1981 tttctccgac tatgaggatt ttcagtttca ggcaaagcac tgtattcaat gtggtgatgt
     2041 catcgacccg tttattttgc tgaaccgctt gcgaaaacat aaaggtgaga ctcccgccgt
     2101 gaactctttg tctcagccgc gtcatcgtga aaatggattg gtgatgaccg ggtaaaacca
     2161 tggttattca ttttggtgag tgagaccaca aaagggtcac attaaagggc aaatctcatg
     2221 aacggcaaaa aatgtagctt ttactggatg tgtggcattc tactgagtct catcatggag
     2281 ggaaatggca ttgccgcgga gatgccgaag gaacccagtg agacctatct catcaatgaa
     2341 gatgtgaata tcattaccgg cctttacact cgggaatact ccttaaaggg agatggagtc
     2401 gtcgattaca aaacggcccg acaaattatt ttttatgaaa ataacaaatt ttggaatacg
     2461 gtcgttgaaa ccgttgaatg gcccttgttc tattggctgg atgacgatcg agatggcgtc
     2521 ttcgatcaat ttgtcgatca gcgggtggaa ggcaatgggg cctatattat tccgtatttg
     2581 ccggtttcgg agaactagtc tatgcaccat ttaagaagaa cagcttttat tttcagcatt
     2641 ctggcctggg cagtccctgc tggatgggcc ttccccttcg cacagccggc tgtggccgaa
     2701 ca
//
LOCUS       DATIAL010000257         3399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1014762, whole genome shotgun sequence.
ACCESSION   DATIAL010000257 DATIAL010000000
VERSION     DATIAL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014762"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..2130)
                     /locus_tag="VLA60_07905"
     CDS             complement(517..2130)
                     /locus_tag="VLA60_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF09322.1"
                     /translation="MSPAPEKVQQDNTDTSRMLKHLEQHHALILEAAGEGIFGLDLEG
                     RHTFVNPAAAKMLGYEPQELIGQPSHSLWHHTKADGTPYPQEQCPNYASYKDGQVHRG
                     DNEVFWRKNGTSFPVEYSSTPIRNDEGALVGAVVVFQDITYRRQCEKSLEQFRRHNDM
                     ILQAAGEGIFGLDLEGHHTFVNPAANILLGYEPGELFGKPSHTTWHHTKSDGSPYPAK
                     ECPIYKAYKDGLVHHGEDEIFWKKDGTSFPAQYTSTPIWDEQGGLAGAVVTFQDITEK
                     KRMAAQLLEEAKLAEVTRVLGDIGHDIKNMLMPVLSGADLLKDELDEQFPTLIREKAK
                     GAETSYANSLDLIRMIVTNARRIQDRVREIADAVKGVTSPPHFTPCRIRDVVDGVLDT
                     LRTYAAEKGITLRTDGLDALPVIDADERRLFNAFYNLINNAIPEVPRGGSVTVGGSPG
                     RRPDTVELTVVDTGRGMPPEVRDRLFTAQAISTKKEGTGLGTKIVKDAIDLHHGHITV
                     ESEEGHGTTFRLVLPITQIVSSPARPGNS"
     gene            complement(2237..>3399)
                     /locus_tag="VLA60_07910"
     CDS             complement(2237..>3399)
                     /locus_tag="VLA60_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSF09323.1"
                     /translation="MLYARSYRWDQGQEADVHVAVTPEQANALADYFEKHLIDPRVTV
                     GAFDVSKVTKKGAEFGAQIYKEHACIGCHTIEENGQLVGGPQSTNLADSGNRYKADWL
                     FRFGMNPQDFTPHSGEFLADATEIGLRDVIGYLVTLGVKDFRYYEPWTSSEFSQASVD
                     RGKVIYKEYCSQCHGAEGKGDGPAASGLNPKPAIHANMAFDQLPMDYLYNVINHGGRS
                     VGKSPSMPYWNLTIGQQGVADVIAFLRATFKGAPSSATTGTSGAGPLGVCPQPRKTPN
                     APADFRKMVNPLPASKAHLSAGKKLFHETAQPLACKQCHGEKGDGRGPLGGGLVPPPR
                     NFACGQTMNDLSDGQLFWVIKKGSPGTGMMAFAGLPDEQVWQLIQYIRTLAK"
BASE COUNT          805 a          848 c          850 g          896 t
ORIGIN      
        1 ggaaggtgtt ctcacccttc ttttttttcg atgattggtt gaaccaatag gagcataatt
       61 ctttctagcg tgtgacgaat gaagttgatt aaaagtaact tgaatagtat ggattgacct
      121 gtgggcaaag atgtcggtct gggtgtgttc aagattgctg aaattggaat aaaaaggatt
      181 ttttttaagt atggactggt tagaaccacc ctcaatgaat gcttctgacc acacatcatt
      241 ccagccagat ctttcgagac aacccacaga tgccgggtgg tgaaatttcg gttttttccc
      301 ctccctgcct ttgctgaagg caagactgtt tgagaaagtc tgatgggtat cgatatgtga
      361 ttgtgtgcaa ggaaaagaag ggaattctta ataagggaag agcgtttcga gcccgaaaca
      421 gatcacagca acaacgggtt gaaaaatcaa tggagacaat ttcctccata ttcgggatgg
      481 gtaaagtatg aatcttcctc gtatcgcatg atcatgtcac gagtttccag gcctggctgg
      541 agatgaaacg atttgagtta tgggaaggac aagacgaaac gttgttccgt ggccttcttc
      601 actttcgacc gtaatgtgac cgtgatggag gtcgatggca tctttcacaa tttttgtgcc
      661 gagtccggtc ccttcctttt tggtgctaat ggcctgagcg gtaaagaggc ggtctcgaac
      721 ttcaggcggc atgcctctcc cggtatcgac taccgtcaac tcgacagtat ccggacgccg
      781 tcccggagat ccgccgaccg tgactgatcc tcctcgtggg acttccggta tggcattgtt
      841 gatgagattg taaaaggcat tgaacaagcg acgttcatcg gcatcaataa caggtagggc
      901 atcaaggcca tcggtccgga gagtaatgcc tttttctgca gcgtaggtgc gaagagtgtc
      961 gagcactcca tccacgacgt ctcgtatacg gcagggcgta aaatgtggag gactggtgac
     1021 acctttaacg gcatccgcaa tttcccgcac acggtcctga atgcggcggg cattggtgac
     1081 gatcatacgt atcagatcca gactgttggc ataactggtc tccgcccctt tggctttttc
     1141 ccggattaag gtggggaact gttcgtccaa ttcatccttt aataaatccg cacccgatag
     1201 cacaggcatt aacatatttt tgatatcgtg cccgatatct ccaaggactc tggtcacttc
     1261 cgcaagcttg gcttcttcca ataattgcgc agccatacgt ttcttttcgg tgatatcctg
     1321 aaacgtgacc acggctccag ccaatccccc ttgctcatcc cagatcggtg tgctggtata
     1381 ttgggcaggg aagctcgtcc cgtctttttt ccagaaaatc tcatcctcac catgatgaac
     1441 caatccatct ttataggcct tatagatggg acattcttta gccgggtaag gagagccatc
     1501 agatttcgtg tggtgccagg tggtgtgact gggttttcca aataactcgc ctggttcata
     1561 gccgagtaag atgttggcgg caggattgac aaaggtgtga tgtccttcga ggtccaaccc
     1621 gaaaatgcct tcaccggcag cctgtaaaat catatcgttg tgtcgacgaa attgttcaag
     1681 ggatttttca cattgtcggc gataggtaat atcttgaaaa accacaacgg cgccgaccag
     1741 ggccccttca tcatttcgaa taggtgtgct ggagtattcg acgggaaaac tggtgccatt
     1801 ctttcgccag aacacttcgt tgtcgcctcg atgcacctgg ccatccttgt acgaggcata
     1861 gttcgggcat tgttcctgag ggtagggcgt gccatcagct ttggtgtggt gccaaaggga
     1921 gtggctgggt tggccgatca actcctgggg ctcatagccc agcattttcg cggctgcagg
     1981 gttcacaaag gtgtgtcgac cctccagatc caacccgaat atcccttcac cggcggcttc
     2041 taaaatgagg gcgtggtgtt gttcaagatg tttcaacata cgggaagtgt ccgtattgtc
     2101 ctgttgtact ttttctggtg ctggggacat gatgattttc ctctttggac tcagggactg
     2161 gagaggatat ctccagtccc caaaatagaa tggcttgtca cactgaagtg aaagtatctt
     2221 gacgaaaaaa ttcaggttat ttggccaaag tccgaatgta ctgaataagt tgccatacct
     2281 gttcgtcagg caatccggca aaggccatca tgcctgttcc aggtgatccc ttcttgataa
     2341 cccagaacaa ttggccgtct gagagatcat tcatcgtctg accacatgcg aagttgcgag
     2401 gaggcggaac caacccacca cccaatggcc ctcggccatc acctttttct ccatggcatt
     2461 gtttgcaggc caatggctga gccgtttcgt ggaagagttt tttcccggcg ctcagatggg
     2521 ccttggatgc ggggagcgga ttcaccatct ttcggaaatc tgcaggggca ttcggagtct
     2581 tacgtggctg gggacacaca cccaacggac ccgcgccaga agtcccggtt gtggcagatg
     2641 acggagcacc tttgaatgta gctcgcaaaa atgcaatcac atccgccaca ccctgttgcc
     2701 cgatcgtcaa attccaatag ggcatcgagg gggatttccc cacactccgc cctccatgat
     2761 tgatgacatt atagaggtag tccatcggaa gctgatcaaa ggccatattg gcatgaatcg
     2821 caggtttagg gttcagaccg gaggctgccg gtccatcgcc ttttccttcc gccccatgac
     2881 attgtgagca gtattcctta taaatcactt ttcctcgatc cacgctggct tgcgaaaatt
     2941 cagagcttgt ccatggttcg taataacgga agtctttaac ccccagcgtc acaaggtacc
     3001 cgatgacatc ccgcaaaccg atttccgtgg catccgctag aaactctcca ctatgcgggg
     3061 tgaaatcctg tgggttcatc ccgaaccgaa acaaccaatc cgctttatac cggtttcctg
     3121 agtcagccag gttcgtgctc tgaggaccac cgaccaattg accgttttcc tcgatcgtat
     3181 gacagccgat acaggcatgc tccttataaa tttgtgcgcc gaattctgct ccttttttag
     3241 tcacttttga gacatcaaaa gcaccaacgg tcaccctggg gtcaatcaag tgtttttcaa
     3301 aataatccgc aagggcatta gcttgctccg gggtcacggc aacatgaaca tccgcctcct
     3361 gtccttgatc ccaacggtaa ctcctggcat acagcatcg
//
LOCUS       DATIAL010000258         4099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1122552, whole genome shotgun sequence.
ACCESSION   DATIAL010000258 DATIAL010000000
VERSION     DATIAL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122552"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VLA60_07915"
     CDS             <1..894
                     /locus_tag="VLA60_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HSF09324.1"
                     /translation="REVGRVLINEGHSVIILTRHAGDHSASAAPQSHFVQWDGVTQGP
                     WVQECDGADVVINLSGAPIADSRWTDKRKQELIDSRVQPTQALLQSIPRWKHKPHTFI
                     SASGVGFYGVRGEEELNESSTPGKGFLSDLCRVWENAAMEAEALGLRVIPIRFGMVLG
                     PDGGALAKMSLPFRLFLGGPVLPGTQYVSWIHRNDLARLILFVMTRSTLIGPVNGVAP
                     ETVTMRDFCTTLGQSMNRPSWFPVPEFLLEIALGELATMLTTGQRVHPQKALKSGFSY
                     SYPTLQEALQAIFCKPSSQTT"
     gene            930..1514
                     /locus_tag="VLA60_07920"
     CDS             930..1514
                     /locus_tag="VLA60_07920"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HSF09325.1"
                     /translation="MNITDKKETRWLLFLMRCFPVLTAGLVIIFGGSSVLGAPLFLEK
                     GSLIPSVTVIGKGGEAISLDTLKGKVTLISVVPQLNTPVCDEQTHRFSERNEGLDQLM
                     SFVTLSTNTYNDQQEFSDKADIHNMTFLSDAPGYHFGTHTGLLLKDLSILHRAVLVLD
                     TRGIIRYVEMVPMGQLPDFDRALQVSRSLLANVS"
     gene            1526..2011
                     /locus_tag="VLA60_07925"
     CDS             1526..2011
                     /locus_tag="VLA60_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSF09326.1"
                     /translation="MTEHILIFFNLLASAVLVGKIVFLSFVTAPVLSRTLDADAFARV
                     VRSLFPPYYGLGMLSAAVGWFTSLALGFFHGFGPVVLVPATLWLGILAIEQYCRTPLI
                     AQINELSDQLKAKQLKGISTPLLQRQRDGLHRRSVQLNSIVLLMGLCVLGFSGLSSTL
                     P"
     gene            2023..2511
                     /locus_tag="VLA60_07930"
     CDS             2023..2511
                     /locus_tag="VLA60_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HSF09327.1"
                     /translation="MLIRLPFNTYANRCLKILGAVVLSAMVCTPWALGEDVRSIDPAA
                     PTNPHASPPGPNTPPPSGIVGLALHITAGRIGEPARLIIRAVHPSGPAARAGLNHGEE
                     ILTVDGVPLTGKTYQEAVGLIRGEVGSSVKLGLTGPQGERTVSLVRISESVLMEQTPQ
                     AM"
     gene            2719..3753
                     /locus_tag="VLA60_07935"
     CDS             2719..3753
                     /locus_tag="VLA60_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HSF09328.1"
                     /translation="MTTRLIDTDLKPHKKTTQPGKNMAECLSDHKPRLGISQCLLGDT
                     VRYDGGHKRDAFLTDVLAPFVEWLPVCPEVEAGLGTPREAMHLAGDPDAPQLLTIRSH
                     IDHTAILQSFSQRRVQELQESDLDGYIFKKSSPSCGVYRVKVYTEKGQPSKQGTGIFS
                     AAFQKAFPLLPVEEEGRLSDAPIRENFIERIFCYRRWKTLFQQHRISRGAIVQFHTRH
                     KYLLLTHSRSHYQDLGQLIAKAGQYTPGDLANHYGPLFMDALKSKATVRKHVNVLQHL
                     AGHFKNQLGPVERAELQETIQDYHRHLTPLAVPLTLIKHYVRILAVPYLVDQVYLNPH
                     PKELMLRNHV"
     gene            3746..>4099
                     /locus_tag="VLA60_07940"
     CDS             3746..>4099
                     /locus_tag="VLA60_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSF09329.1"
                     /translation="MSNSPKDSQTYRIKSVASTTGLSTHVIRKWEERYHLVHPQRGPN
                     GYRLFTDDDIQFLLYLKSQLDHGESIGQLAQAGEQELRHSMDQVPLNLSGIAPIYWND
                     TQEMIRLARHQDVQAI"
BASE COUNT          987 a         1100 c         1011 g         1001 t
ORIGIN      
        1 cgagaggttg gccgggtgct gattaatgaa gggcattccg taatcattct cacccgccat
       61 gcaggggatc acagcgcgtc tgccgcccca caaagtcatt tcgtccagtg ggacggcgta
      121 actcagggac catgggtgca ggaatgcgac ggcgcagatg tcgtcatcaa tttatccggt
      181 gcaccgattg cggattcccg ctggacggac aagcgaaaac aagagcttat tgacagccgg
      241 gtacagccga ctcaagcctt gcttcaatcc atcccaagat ggaaacacaa acctcacaca
      301 tttatttccg cctctggagt cggcttctat ggagtcagag gtgaagaaga gctgaatgaa
      361 tcttctactc ccggcaaagg ctttctttcg gatctttgtc gggtttggga aaatgcggcc
      421 atggaagccg aagccttggg gcttcgggtt attccaatcc gattcggtat ggtcctagga
      481 cccgatggcg gagctctagc aaaaatgtct ttgccgtttc ggttgtttct gggcggtccc
      541 gttttacccg gaacacaata tgtttcgtgg atccaccgga atgatcttgc ccgtctcatt
      601 ctttttgtga tgacccgttc tacgctgatc gggccggtca atggagtcgc ccccgaaaca
      661 gtcaccatgc gggacttttg taccacatta gggcaatcca tgaaccgtcc atcctggttc
      721 ccggttccgg aatttttgtt ggaaatcgcc ttgggagagc ttgccacgat gctgacgacc
      781 ggccaacgcg ttcacccaca aaaagccctg aagagtggat tttcctattc ctatcccacc
      841 ctccaggaag cgttacaagc aatattttgc aaaccatcat cccagacgac atagctggac
      901 catgaccatt taagctaagg agcgaaccga tgaacatcac agataaaaaa gaaacgcgtt
      961 ggttactctt tctaatgcgg tgctttccag ttttgactgc cggattggtt attattttcg
     1021 gtggatcctc cgtcttagga gctcctctct ttttagagaa aggcagcctc atcccctctg
     1081 tgacggttat cggaaaaggt ggagaggcca tttccctcga tactctcaag ggaaaagtga
     1141 cgttaatcag tgttgtgcca cagttgaata ctccggtgtg cgatgaacag actcaccggt
     1201 tcagtgaacg caatgaaggc ttggatcaat tgatgtcatt tgtcaccctg agcacgaata
     1261 cctataacga tcaacaggaa ttttctgata aggcggatat tcataatatg acatttttat
     1321 cggacgctcc tggttatcac ttcggcacac acacaggcct tctcctgaaa gacctgagca
     1381 ttcttcaccg cgcggtgctt gtgctggata ccagggggat tattcgatat gttgaaatgg
     1441 tacccatggg acagctgcct gattttgacc gggccttgca ggtgtcgcga agcctgctcg
     1501 ccaatgtctc ttaagaggtg acgtcatgac tgaacacatt ctcatttttt tcaatttgtt
     1561 ggccagcgcc gtgttggtcg ggaaaattgt ctttctttcc ttcgtcacag cacccgtgct
     1621 ttcccggacg ctggatgccg atgcttttgc acgggtcgtg cgaagtctat ttcctcccta
     1681 ctacggattg ggaatgctca gtgcggccgt tggatggttc accagcctcg ccctgggttt
     1741 tttccatggt ttcggacccg tggtacttgt acccgctacg ttgtggttgg gaatactggc
     1801 gatcgagcag tattgccgaa ctcctctgat tgcccagatt aatgaactta gcgaccagct
     1861 caaagccaaa caactcaaag ggatttccac acctctcctt caacgacaaa gagatggcct
     1921 ccatcggcgt tccgtgcaat taaattctat tgtgctgctc atgggtttgt gtgtcctcgg
     1981 tttttcgggt ctttcatcaa cactgccata aaggagattt ctatgctgat acgcttaccc
     2041 ttcaatacct atgcaaatcg gtgtctcaaa attttaggag ccgtggtcct ttcagccatg
     2101 gtatgcacgc catgggcctt gggtgaggat gtccgatcta ttgatccagc ggctcccacg
     2161 aatcctcatg ccagtcctcc aggtcccaac acacctcctc cctcgggaat tgtgggactg
     2221 gccttgcata tcacagccgg cagaattgga gaaccggctc gattgatcat tcgcgccgtg
     2281 catccgtccg gacccgccgc ccgggccgga ctgaatcatg gggaggaaat tctgaccgtc
     2341 gacggggtcc ccctaaccgg gaaaacctat caggaagcag tcgggctcat tcgtggagaa
     2401 gtggggagca gtgtcaaact aggactcaca ggtccccaag gtgaacgcac cgtcagtcta
     2461 gtccgaatca gtgaatcggt cttaatggag caaacacctc aagccatgta agcagagtaa
     2521 aagtatgcag gacagaattc catccccctg ggtcaccgaa tccgaagggt tgatgaagct
     2581 gaaatgtatt gtgtcacttc gcagactcgg gagacttatt gtgcatgaac gggtattcaa
     2641 cgccggagaa ggattgcgaa atctgcaaca cgggtaaagc ggatccagca cccgttagga
     2701 gaaggattac acatccatat gaccacacga cttatcgaca ccgatctcaa acctcacaaa
     2761 aaaacgactc aacccggcaa gaatatggct gagtgccttt cagatcataa gccgcgattg
     2821 ggcatcagcc aatgtctgct cggcgacacg gttcgatacg atggcggaca caaacgggat
     2881 gccttcctca ccgatgtgct ggccccgttt gttgaatggc ttccggtctg tccggaagtc
     2941 gaggccggac tgggcacccc gcgagaggcc atgcatttgg caggggatcc cgatgcgcca
     3001 caactcctga ccatacgttc ccacattgat cacaccgcta tcctgcagtc ctttagtcag
     3061 cgccgggttc aggagttaca ggaaagtgat ttagatgggt atatttttaa aaaaagttcc
     3121 cccagttgcg gagtctatcg ggtcaaggtc tataccgaga aagggcaacc ttctaaacag
     3181 ggtaccggta ttttttcagc cgcctttcaa aaagcgtttc ccctgctccc tgtggaggaa
     3241 gaaggacgtt tgagcgacgc accaattcgg gaaaatttta tcgagcgcat tttctgttat
     3301 cggaggtgga aaaccctgtt tcaacagcat cggatcagcc gtggggccat tgtccaattc
     3361 catacccgcc acaaatattt attgctcacc cacagccgtt cgcattatca ggaccttgga
     3421 caactcatcg cgaaagccgg ccaatataca cccggtgacc tggccaatca ctatggcccg
     3481 ctctttatgg acgcgctgaa aagcaaggcc acagtgcgaa aacatgtgaa tgtactccag
     3541 catctggcgg gccactttaa aaaccaactg ggcccggttg aacgggccga gttgcaggaa
     3601 accattcagg attaccaccg gcatctcacc cccctggccg tgccgctgac gcttattaaa
     3661 cattatgtgc gcatcctggc cgtaccctat ttagttgacc aggtctacct caacccgcac
     3721 cccaaagaac tcatgttgcg caaccatgtc taacagtcca aaagattccc aaacctatcg
     3781 gattaaatct gtggccagta ccactggact cagcacccat gtgatccgga aatgggaaga
     3841 acgctaccat ttggtccatc cgcagcgagg ccctaacggc tatcgactct ttaccgatga
     3901 tgatattcaa tttctcctct atctgaaatc acaattggat cacggtgaat ccattgggca
     3961 attggcccaa gccggggagc aggaactccg acattcgatg gatcaagtcc ctctcaatct
     4021 ttcgggaata gcccccatct attggaatga cacccaggaa atgattcggt tggcccgcca
     4081 tcaagacgtc caagccatt
//
LOCUS       DATIAL010000259         4000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_478021, whole genome shotgun sequence.
ACCESSION   DATIAL010000259 DATIAL010000000
VERSION     DATIAL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478021"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VLA60_07945"
     CDS             <1..316
                     /locus_tag="VLA60_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HSF09330.1"
                     /translation="PSADAYLMKLILHDWNDDECVRILNNQYQAANPGGRVFLVEHVI
                     PDPGTPHFSKLFDIHMMCWGTGRERTQEEYATLLKKAGWKYMTTWYSASRAMGVVEGA
                     KV"
     gene            795..3902
                     /locus_tag="VLA60_07950"
     CDS             795..3902
                     /locus_tag="VLA60_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09331.1"
                     /translation="MVKHHFLSYSTIDGLEFALDLHDKLEGGYPPIPLWMDKHNLQPG
                     QDWDTQIVKAIRECDSLIFIMTTDSLRDNSVCKNEWTRALKYKRPIVPIRLHADAEMP
                     FRLGNRQFIDFSTDPSAGLAQLRSHLKWLSSPEGKIQTLQDQLADVQRDLQRSTGAMT
                     THIQEEIDQLELTIAIQNTKLQNPHNTIPSDPASLKRDLIQEEANSQSQRTPSYTHII
                     NDPPVVVPTYFQNRQKEILLIEDFLNDDTKNLLNVVGRGGTGQTTLVCRVLQSQTESQ
                     GSMEEGNPSIDRMVYLSSTASQGTPWLNLFTDLCRFLPEETARALQQLASQPNASSYD
                     IMQSLLEALPQGRTIVFLDNVQHIIDPETRNFIDQELEKALRALLDDGRSHLKFILTT
                     HVAPKALTLVHPARQRSLHIQEGLKPPYTVNILRQMDADGTVGLRTAPDALLEEAGER
                     TRGFPRALEALYAILSADRETTLEEILADTRRLLPEHVLEVLAGEAFTRLDSLGKRVV
                     QALAIMDTPISPVAINYLLESYLPGLDSEPILKRLLNMQFVRKQGGNFSLHAVDREYA
                     LSRIPPGEQNDQGMNNPPFTQRALFHRAANFYRDIRKPQNEWNMLDDLTSQIREFDLR
                     CLGKEYEPALRLLLTFDFDYLLRWGHYRLMANLHERVHGHIHDQKLQQLHLANLGTAY
                     YRIGDLSKAIQYHEEALTLAQHNQDPSGESIALANLGLCYRGLGKTLEAIDNYEAALP
                     ILRELNLRDAVATVLMNLGTAYSALGRIQQAVTVLEEANAIYLETGNQNNRAMALVCL
                     GEVQGHLGKTQDATHYASEGLALARAMANPFVEAAALCILGELERDSKNWDAAIARYQ
                     EAIQKADFISNAEIRNIARTRLCMVLLLSDNLIDARKTIEDALQIEAPSEKHVASAMY
                     GIVLLRQGNHPQAKQQFLQTLTHTVALLNHCSQDIWALESQALAQCGLAICDQSPELT
                     SSAIETFQKVRELVHDKGTLQRMVKLFEALCLANTQGILTDELHLAANTESWLKKAGS
                     VEGPI"
BASE COUNT         1140 a         1061 c          861 g          938 t
ORIGIN      
        1 tccgagcgcc gacgcctatc tcatgaaatt gatcctgcat gattggaacg acgacgaatg
       61 cgttcgaatt ctcaataacc agtaccaggc agccaatcca ggtggccggg tcttcctcgt
      121 ggaacacgtc atccccgacc ccggcacccc ccatttttcc aagttgtttg acatccacat
      181 gatgtgctgg ggaacgggaa gagaacgcac acaggaggaa tatgcgactc tattgaagaa
      241 agctggttgg aaatatatga cgacctggta ctctgcctcg cgagcgatgg gagtggtcga
      301 aggagcgaag gtctaacagc ttttgaaaaa tcctgttctc catgaggcct ccggcatatt
      361 gcatgatgct tacaggaaaa cacgggagag taacggcacc gctaacccat ttccactaaa
      421 gattctcaaa gccagatctt tggtccgtat gccgccaaac tgttcaaagt gaatgtcgac
      481 acgaaactga aggatttacc caaggattat ttgtgccacc tcaaaatggc aatctggaag
      541 aaggccatct ctcagccact atgccttcgg ctggatttca aaaaaaacaa attcaatccc
      601 cactgaaaga gacttcttag acccacagtc atttctctca ttaatattta tttcccttga
      661 gattcatggc acattcttct acaattttct atctgcattc ttaaattcct tcatatcccg
      721 ttcgtgtcaa aaaacgtccg ttttgatttc catccgtcgc agggtaaacc ctagcgtttc
      781 ttaggataac atgtatggtg aaacaccact ttctcagtta ttccaccatt gatggacttg
      841 aatttgcgct cgatctgcac gacaaacttg aaggggggta ccctccaatt cccctctgga
      901 tggacaagca caatctgcaa cccgggcaag attgggatac tcaaattgtt aaagccattc
      961 gagaatgtga cagcctgatt tttatcatga ccacagatag cctaagggat aattcggtat
     1021 gcaaaaacga atggacccgg gctctgaaat acaaaagacc catcgtcccc attcgacttc
     1081 acgccgatgc cgaaatgccc tttcgattag gcaatagaca atttattgac ttttccactg
     1141 atccttcagc aggactcgct caactccgat cccacctcaa atggttaagc tctccggaag
     1201 gaaaaattca gacactccag gatcaactgg ctgatgtcca acgagacctc caacggtcga
     1261 caggagccat gaccacccat attcaggagg agattgatca acttgaactg accattgcca
     1321 tccagaacac gaaacttcaa aatccacaca atacgattcc ctctgatccc gcatctctga
     1381 aaagagatct cattcaggag gaagccaatt cacaaagcca gagaacgccc tcttatactc
     1441 acatcataaa tgatcccccg gtggtggttc ctacctattt tcaaaaccgc cagaaagaaa
     1501 tattactcat tgaggacttc ttaaatgatg acaccaaaaa cttactcaac gtggttgggc
     1561 gcgggggaac cggccaaacc acgttagtct gccgagtttt acaatcccaa acagaaagtc
     1621 aagggtcgat ggaggaagga aacccttcaa ttgacagaat ggtgtatttg agctccaccg
     1681 cctcacaggg taccccctgg cttaatcttt ttacggatct ctgccgtttt ttgcctgagg
     1741 aaacagcccg tgctcttcaa caattagcct ctcaacccaa tgccagttca tatgacatca
     1801 tgcaaagcct cctggaggcc ttaccacagg gcagaacgat agtctttctc gataatgttc
     1861 aacatatcat cgatcccgag acccggaatt tcatcgacca agaactcgag aaggcccttc
     1921 gtgcactgct agacgatggc cggtcacatc tcaagtttat tctgacaacc catgtcgccc
     1981 caaaagcctt aaccttagta catccggcac gccagcgatc tttgcacatt caagaaggac
     2041 tcaagcctcc ctatacagtg aatattttgc gtcaaatgga cgccgatgga acagttgggt
     2101 tgaggacagc ccctgatgcc cttctggaag aagccggaga acgaacaaga ggttttccac
     2161 gagccctcga agcgctctat gccattctgt ccgcagatcg tgaaaccaca ctggaagaga
     2221 ttttggcaga cacccgtcgt ttgctacctg agcatgtctt agaagtcctg gccggtgaag
     2281 catttacccg tctggattca ttaggcaaaa gagttgtaca agctctcgcc atcatggaca
     2341 cgccaatttc ccctgttgcc attaattacc tgctggaatc ctacctccca ggccttgatt
     2401 ccgaaccaat cctcaaacgc ctgctcaata tgcaattcgt tcgcaaacag gggggaaatt
     2461 tttccctcca tgctgttgac cgggaatatg ccctctcacg aattccccct ggcgaacaga
     2521 atgatcaggg aatgaacaat cccccgttca cacaaagagc actctttcat cgtgcggcga
     2581 acttctatcg ggatattcga aaacctcaga atgaatggaa catgctggat gatctcacct
     2641 cacaaatacg cgaatttgac ttgcgatgtc ttggcaaaga atatgaacca gccttgcgcc
     2701 tgttactgac attcgatttt gattacctcc tccggtgggg acattatcgc ttgatggcca
     2761 acctccacga acgcgtacac ggtcacatac acgatcagaa gttgcaacaa ttgcatctgg
     2821 caaatttagg aacagcctat tatcgaatcg gagacctctc caaggccatt cagtatcatg
     2881 aagaagctct cacattggcg caacataatc aggatccatc cggggaaagc attgccttgg
     2941 ccaatctggg gttatgctac agaggattgg ggaaaacgct tgaagctata gataattatg
     3001 aggccgcgct gcccatcctc cgtgaattga accttcggga tgcggtcgcc accgtattaa
     3061 tgaacttagg cacggcctat agtgccttag ggagaatcca acaagccgtc acggttcttg
     3121 aggaagcgaa tgccatttat ctggaaacgg gaaatcaaaa caatcgagct atggcattag
     3181 tctgtcttgg ggaagttcag ggccatctcg gcaagaccca agatgcgacc cactatgctt
     3241 ccgaaggatt agctttggcg agagccatgg caaatccttt tgtggaagcc gcggccttgt
     3301 gtattctggg ggaactcgaa agagattcta aaaactggga tgcagccatt gccaggtatc
     3361 aggaagctat ccaaaaggca gacttcatca gcaacgcaga gatccgaaat atcgcgagaa
     3421 cacgattgtg catggtgtta ctcttatctg acaatctcat cgatgcacga aaaacgattg
     3481 aagacgcctt gcaaattgaa gcaccatccg aaaaacatgt ggcgtcagcc atgtacggga
     3541 tcgtgttgct tcgacaggga aatcacccac aagccaaaca gcaattcctc caaaccctca
     3601 cacacactgt agcgctcctt aaccattgct cccaggatat ctgggcactt gaaagtcaag
     3661 cactcgctca atgcgggcta gctatttgcg accaaagccc cgagctgacg tcttccgcca
     3721 tagagacctt tcaaaaagtc cgtgaacttg tccatgacaa aggaacactt caacgaatgg
     3781 tgaagctatt tgaggcgtta tgccttgcca acactcaagg cattctcaca gatgaactac
     3841 acctggcagc aaacaccgaa tcatggctga aaaaagcagg gagcgtagaa ggaccaattt
     3901 aagtgcaaat ggaagacacg cgttctttac ctttcccatt cctaccaaac aacagaaaac
     3961 accgcagtac cctgccttga ccgaagatct caaagccaac
//
LOCUS       DATIAL010000260        25867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_586099, whole genome shotgun sequence.
ACCESSION   DATIAL010000260 DATIAL010000000
VERSION     DATIAL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 25867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25867
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586099"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VLA60_07955"
     CDS             <1..586
                     /locus_tag="VLA60_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HSF09332.1"
                     /translation="QAGQAAKVPGLCQLLAPHRHSASKPLRMLIMGIPNVGKSTLMNS
                     LLKRRLAKVGNEPAVTKVQQRHTLNDHMTLIDSPGLLWPKIEDPLVGLMLATIHAVTA
                     KVMVEEEIAEFLVTILLARYPTLLTKRYGFLTEGLDSTGVLEAIATKRGCLRKGKGGE
                     LDREKAAKILLTEFRNGTLGRISLETPDTRDARL"
     gene            complement(1211..1570)
                     /locus_tag="VLA60_07960"
     CDS             complement(1211..1570)
                     /locus_tag="VLA60_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09333.1"
                     /translation="MGPTKAVVKDCGIYDAKTGNLIKDGFPTPESIQDYAAHHYLVLP
                     VVNKDCQPWLLDGQPIFCLRGTRYENVKDEVLHLARCPDCGGMGIRDDEPVVESDCIR
                     CVSCGHEFDTRLEMMES"
     gene            complement(1795..2118)
                     /locus_tag="VLA60_07965"
     CDS             complement(1795..2118)
                     /locus_tag="VLA60_07965"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HSF09334.1"
                     /translation="MPIYVSLVNFTHDGLMTMKAKGVQRSDMVKKNVESLGGKMLHAF
                     YCLGEYDVVAILEFPNNRAAMKAGVLNASMGHIRIKTMPAVSRDEWKSVLSETWGKPK
                     MKKRG"
     gene            complement(2319..2690)
                     /locus_tag="VLA60_07970"
     CDS             complement(2319..2690)
                     /locus_tag="VLA60_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HSF09335.1"
                     /translation="MTASSHPHAITIMPNPNRVKVTFNGTVVADTSRALTLREGPVPP
                     AQYIPREDVTMSCLRPTTHSTHCPFKGDASYFSVSVKGKTAENAVWTYETPLPSVADI
                     KDYVAFYPEKMDAIEELPPGS"
     gene            complement(2794..3552)
                     /locus_tag="VLA60_07975"
     CDS             complement(2794..3552)
                     /locus_tag="VLA60_07975"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF09336.1"
                     /translation="MMNQSTRFEIPGADSKRIVGDRLSGQERQLLFVTGFLSKRWGNK
                     SKALADLCKERDWGFCCFDFRGNGESEGAFADYTLFDWLADARCVTTMLADGPPVTIV
                     GSSLGGWLAWLLGQEFPHVQQLVLLAPAFNMMGKRAQDISPERRQRWQETGWMPWDDD
                     ALHKNFPLSWKWVEESETLWARRLDSPKRVSTRIVHGLQDVVILPKGSWEFTEHLLTQ
                     DPQYPIELLLKTGDHRFSSPANLQTFLDVAQQLQ"
     gene            complement(3637..4626)
                     /locus_tag="VLA60_07980"
     CDS             complement(3637..4626)
                     /locus_tag="VLA60_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HSF09337.1"
                     /translation="MPCSWSRAKSLQATWRFYGAALAGMTLCLGCASSPSEVAPVSCE
                     VSHLQKTDLPERGISAHRGGQLGCPVNTNGAFQRAICLGVHQIELDVRSTADAVLVVS
                     HDDRVTDEQGRTLRISESTLAEVQSLQLEACAGEVAGQRIPTFEEALAVMPHNIWINV
                     DIKENNPLVGRLAAEMVANAQRFNQIIFGARDDTDPAVRRVAGEADEESWVANMSREM
                     FRSQYVDTTINSCDEFIQLSFFRGRPSVETMDQLNQAGIRVNYSWLGDEDESELRQDL
                     GDLFARGVDFVLVDHIGQAMEGACALGITPVVPHWNGTSPFTCSEPSRCAQAR"
     gene            complement(4676..5221)
                     /locus_tag="VLA60_07985"
     CDS             complement(4676..5221)
                     /locus_tag="VLA60_07985"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HSF09338.1"
                     /translation="MSLFVDRMIRAAKLDVHLYEEVEADRRSMGQAMGVVLLSSLAGG
                     IGFMQEAGLMGLVIGTVGSLLGWYVWAFMTYLIGTKLLPEPQTHADHGELLRTIGFSS
                     APGLIRVFGIFPALSGFVNLLAGVWMLVAMVIAVRQALDYQSTYRAIGVCLIGWIIQA
                     ILLALLVTTMGGVPELMLEQP"
     gene            complement(5464..5952)
                     /locus_tag="VLA60_07990"
     CDS             complement(5464..5952)
                     /locus_tag="VLA60_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HSF09339.1"
                     /translation="MMKRFSLALPFVFGILFLIGCTSLPALSVQPESPEVLLVNITPL
                     DATMFEQRLQVDLRVRNPNDFDLEVTGLDFTLHLNDQRLARGLTNKASTIPRLGDAVI
                     SVETTTSTLDVIRQLLQLSQRQEVSYQVDGILYVQGARLPFENKGVLLDTNEISGSSP
                     GS"
     gene            complement(6163..7869)
                     /locus_tag="VLA60_07995"
     CDS             complement(6163..7869)
                     /locus_tag="VLA60_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09340.1"
                     /translation="MSIKLYSSKYAPGRSCLRYFLIAIFVMALPQVSLGEMDHTSSSS
                     EGNAALGQLGSIEFPASGSREAHPHFLRGVAALHSFWYEEAIDAFRRATTIDPNFVMG
                     YWGEAMANNHPVWQEENVEAARAALGKVSDTASATVRERQYLQAVNTLFGEGDKLARD
                     QAYAARMESLASEYPEDLEATCLYALSLLGLAVHYDEKPYLQEKYRVQAGALALGVYG
                     VNPNHPCAAHYTIHAFDDPIHAILALPQARRYAKIAPEAHHAQHMPAHIFVQLGMWDQ
                     AAASNKAGWKSSQAWVKDKQLPLSLQDYHSLYWLQYAYLQQGRYNAAAALILEKQQDM
                     AQSSSGEQTQVLGYDRKVNRNYDQMVAAFIIETERWDLADQAWNVNDYRFGDESPSIS
                     VYIREFAQSMATLRNQSIAHRVHPGINSPVQEPSTESIEPITLQIWKLQIAALKHLIN
                     GDHSQALRMLDQATALEESLPPPSGPPDLIKPSHELYGELLLGIDRPKEAKEQFERSL
                     LWHPNRARSLLGLARAAAQLGHIQATRQVYSNFLNIWNQADSDLPEVNEAKQFIQQAV
                     QQ"
     gene            complement(8147..8344)
                     /locus_tag="VLA60_08000"
     CDS             complement(8147..8344)
                     /locus_tag="VLA60_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HSF09341.1"
                     /translation="MAHTYDVYLLTNWNNNVMYAGMTNNLERRMYEHKKKLVKGFTQK
                     YNRFTQKYNINKLVYFEKLKK"
     gene            complement(8399..9424)
                     /locus_tag="VLA60_08005"
     CDS             complement(8399..9424)
                     /locus_tag="VLA60_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09342.1"
                     /translation="MYRLHQTSTIIIGLIIPLVFSGCTNHPTKAEAASLKSVEMKEWQ
                     VPWDQTRPRDPYVDQQQRVWFVGQGGDYIAVLDPELGQFHQFKLEPGTGPHNLIVDQE
                     GQVWYAGNRAAHIGKLDPSTGKIITYPMPDPKAQDPHTLVFGHDRDIWFTVQQGNFVG
                     HLDMATGKIQLMPLPTAQARPYGMVLDPTHSPWFTEFGTNKLGTVDRATMTVQEFALS
                     RTETRPRRLAVTSDGVIWYVDYAEGYLGSYNAKTGDIQEWLAPGEADAKPYGMTVDDQ
                     DRVWFVETGAHPNRLVGFDTRTHTFMSLTDIPSGGGSVRHMVYHQPTKTIWFGTDANT
                     IGRAKVP"
     gene            complement(9548..10618)
                     /gene="arsB"
                     /locus_tag="VLA60_08010"
     CDS             complement(9548..10618)
                     /gene="arsB"
                     /locus_tag="VLA60_08010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HSF09343.1"
                     /translation="MTVSAPRRLSFLDRYLTGWIFLAMAAGVTIGSVFTGVPALVNSL
                     SLGTTNIPIAIGLMLMMYPPLARVKYEELPLIFRDWKILLLSLVQNWVIGPLLMFGLA
                     VTFLHDSPEYLTGLILIGLARCIAMVLVWNQLAEGDNQYVAALVAFNSLFQLIFFSGL
                     AWFYLDILPALLGLAVEVVDVSFQTIAESVFIYLGIPFVFGFLSRWIGIRQKGRVWYE
                     QEFLPKIGPLTLMALLFTIFAMFTLKGGTVLSLPGDVVRIALPLILYFVIMFLVSFSM
                     GKLIGADYGKTTAISFTAASNNFELAIAVAIAAFGLGSSVAFATVIGPLVEVPVLLAL
                     VHVALWLKKKWFAGDPASVFLP"
     gene            complement(10615..11037)
                     /locus_tag="VLA60_08015"
     CDS             complement(10615..11037)
                     /locus_tag="VLA60_08015"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HSF09344.1"
                     /translation="MSIELIKVLVLCTGNSCRSIMAEALINHLGQGRYRAWSAGSHPA
                     GYVHPGSISTLKRHGIDPGQPYSKSWDDMISQPFDLVITVCDQAAGEACPIFSGQPRK
                     LHWSIPDPGRVIGTEDQINAAFDRTFFLLKSRIEEVLS"
     gene            complement(11037..11510)
                     /locus_tag="VLA60_08020"
     CDS             complement(11037..11510)
                     /locus_tag="VLA60_08020"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HSF09345.1"
                     /translation="MKRLHIHIGVEQLEEAIVFYSKLFGAEPVKRKPDYAKWMLEDPR
                     VNLAISTRTSTKGVDHLGIQVEEGQELDEIRQRLQVGNLPVAEEGETLCCYAKSDKSW
                     VVDPAGVPWEAYRTMEDAEIFSTHSAHTETACCEPAFPASAKTNMPVQESNGCCS"
     gene            complement(11535..11885)
                     /locus_tag="VLA60_08025"
     CDS             complement(11535..11885)
                     /locus_tag="VLA60_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSF09346.1"
                     /translation="MEINDALLAFAALSQDTRLRVFRLLVEYGPAGAPAGTLSEALGI
                     PHNTLSFHLSHMSKAGLVVSRREGRSIIYSANFEFFADLIRYMVKDCCREDMASIRDN
                     KKKKCSIIELSNCC"
     gene            complement(12110..12478)
                     /locus_tag="VLA60_08030"
     CDS             complement(12110..12478)
                     /locus_tag="VLA60_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09347.1"
                     /translation="MSKNTLIVDDDEMSRRLLRLVLEYDGCHCVEAENGAAGLSLIES
                     QPFDIIILDNAMPVMTGMEFLNRLQLRPESPYVPVIMVTGLLNAEIREKAIRLGVYAI
                     LGKPYDFGELRSMVAQLLCP"
     gene            complement(13516..13905)
                     /locus_tag="VLA60_08035"
     CDS             complement(13516..13905)
                     /locus_tag="VLA60_08035"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HSF09348.1"
                     /translation="MRSQKSDNEESPSKGSASRLPKSKMVVRTTKPKSGNKGKSHSLA
                     QPVEPQNGAGTIHFPTEQSVGLSEWELQERIAKRSYELFEQRGGQHGNDQADWFQAAK
                     EVLTLNGVGKNEWSDREKSDSRVNRAC"
     gene            complement(14048..14332)
                     /locus_tag="VLA60_08040"
     CDS             complement(14048..14332)
                     /locus_tag="VLA60_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSF09349.1"
                     /translation="MNLKMFVGGLPFAMTDAQLRELFAPYGVVKSAQVIMDKLTGRSR
                     GFGFVEMSREEEVQAAIAAWNDKVYEGRTLLVNEAKPRMARTGSGSGPRW"
     gene            complement(14478..14933)
                     /locus_tag="VLA60_08045"
     CDS             complement(14478..14933)
                     /locus_tag="VLA60_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09350.1"
                     /translation="MMMSERILVVDDEAGSARIIADAIAWLGFDVVTASNGREGLTVL
                     GTMPFDGILLDLEMPVMNGWTMLDELRWQGYDVPVIVMSQEANQKKLRDLLHEGAQDF
                     LVKPFNPYLLLQKSLRHFSYRTSLEKTPVREKNGAGQAGLYQAKRILVA"
     gene            complement(15186..15335)
                     /locus_tag="VLA60_08050"
     CDS             complement(15186..15335)
                     /locus_tag="VLA60_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09351.1"
                     /translation="MNQLESPGTGKSVPMKKEALPDNQRKSRDYSHAIPSDIGGGKVP
                     KRFLI"
     gene            complement(15431..16426)
                     /locus_tag="VLA60_08055"
     CDS             complement(15431..16426)
                     /locus_tag="VLA60_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF09352.1"
                     /translation="MMGSGKQNDHVWSIILAGGNGERLGLATCHWFGDHKPKQYCTFV
                     GTRSMLQHTIDRADRLVPSERRVTVISQAHQHLAWEQMADRAAGTIISQPLNRNTVPG
                     IFLPLTYVRARDAKATVVIYPSDHFVTPEARFLEIVGRAVSATEWLTDRLVMLGVKPD
                     RTEPDYGWIEVGREIGWVSGQKIRAVHSFLEKPDLTEWGTQSRSGMWWNTMVVAAKAE
                     TLWKLGWQCFPEMMPLFERLGHAIGTSYEGTVLESIYRVMPAWNFSSHLLARVTNHLA
                     MLEMAGVSWSDWGSPERILDDIHRFGLQPNFSLERAGQMRKPRRLDMEPATALQT"
     gene            complement(16522..16740)
                     /locus_tag="VLA60_08060"
     CDS             complement(16522..16740)
                     /locus_tag="VLA60_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09353.1"
                     /translation="MRGQCFRCGGWMIETFVEGEAVCRDDPAGIWKCVNCGEVIDSQI
                     LANRQFGSHTQPKTPIPSHASLPLGRVS"
     gene            complement(16929..17561)
                     /locus_tag="VLA60_08065"
     CDS             complement(16929..17561)
                     /locus_tag="VLA60_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF09354.1"
                     /translation="MVKILVADDHAVVRQGVKQILAEDFKDAVVGEASNIHEVLDLVR
                     KRKWDIVILDMTMPGRSGLDALKDLKQLHPKLPVLVLSMHPEDQFAVRVLKAGAAGYL
                     TKESAAEELVTAIRKVLLGGKYISSALAEVLAMSLSDDLGSPPHLTLSDREYQILSLI
                     ASGKTARDIAQELSLGVSTVSTYRSRILQKLKMKNNAEITRYAFQHQLIP"
     gene            complement(17641..19506)
                     /locus_tag="VLA60_08070"
     CDS             complement(17641..19506)
                     /locus_tag="VLA60_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF09355.1"
                     /translation="MRMPNKDTQRILIIDDEPDDVELTGEALLQVWPGSIIAGAGTAE
                     AGLAKARASSWSLILLDYKLPGKSGLEILKELRSLCPESSIILLTGHGDEATAVQVML
                     AGADYYLRKSTNFLLELPMVVREVLEKRHLRLRIKDTEERYHRLIEHITDIVFELDAL
                     GCFRFVSPAVLPILGYAPEEMKGVPVSHFLHPDDLPRSAHWFHKEGMERQATRGSSIR
                     FLMKSGGIREIEVDATVIYDHRKQFSATVGIARDVTERKKAEASLARLRYKYELLLNS
                     AHDGIYGIDLEGKTILVNPAAASMIGCTREELHGQSMHDILHHSKSDGTPFPVEECQI
                     HQAFRDGKPYHVPEDVFWKKDGSPFWVEYTSNPIRENDRIVGAVVTFRDITERKRSDE
                     RLKESHEQLRDLAAHLETVREEERTRVAREIHDELGSALTCLKMDLAWMAKRIPMTNS
                     RETASQLAPKIHSMAKFVNEMVQLVQKVSTELRPAVLDELGLGPAIEWQAKEFQCRTG
                     ITCTLDIYPESLVIDSERATTVFRILQEILTNVTRHADATSVSIYIKRSVRYLELKVT
                     DNGCGITLMQISSPKSLGLVGMRERALLWGGEVTIVGEPEKGTTVFLQLPLNREG"
     gene            complement(19669..20112)
                     /locus_tag="VLA60_08075"
     CDS             complement(19669..20112)
                     /locus_tag="VLA60_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09356.1"
                     /translation="MTINVIDILLIEDNVDDIELTREALRAGKIANNLTVIRDGQEAM
                     KHLFGNGDPPLPPERFPKLILLDLRLPKVDGIDVLRRIKKEPVLCRVPVVVLTTSKRD
                     EDIVRSYDLGVNSYIQKPVLFDKFVETVKTLELYWVLTNTPPPYL"
     gene            complement(20177..22324)
                     /locus_tag="VLA60_08080"
     CDS             complement(20177..22324)
                     /locus_tag="VLA60_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09357.1"
                     /translation="MMQLPKSSYQWLSWLRILIVVGVLVHGAVAVHQLENQTLEAAGR
                     SLTRVAVDIADKLDLLLFERYGDIQTIAKSEVFHGRDVHTMNNYLDLFKKAYPIYRWI
                     AVTDPQGRIIAASNRSNVGEDRSRETWFQDVRDKGGIHIQNAERSRDAGGVLAVAFSA
                     PILDADAKFLGAVTARVGLPDLRKVFDRTVIPVQMPEGPSAMGEWHLLDKDGNLLADS
                     LLQEEGKVNLRKLGVQSAFMNASGQAGYVEEMHKRRGVEILRGYARTKGIGEFPGLNW
                     TVLVNLDRSAILAPFHRIVWNLGLAGGVIFFPLVALLYWLTYRLRQEYGQAQTEAVRA
                     LTAEREYRAARDQLADVLDHAPDPIFFSDTEENITRFSRGAEHVLGYAAEAVVSKPVT
                     ELFMDPGQWKPLVDELTVHGEVIGRDVVFRNPKGYPVHISLTLSVLRNAEGQPAGTVG
                     LCKDVTAAKRNEESLRISNEELESFVYAISHDLQAPLRGIQGFAVLLLKRAGERLEHQ
                     ERHYLERIRKGAERMEALIRDLLEYSRIERITHPWELVSMEQIVLQVRMDLEDRIRQM
                     HAELRIEDSLPWVYGDRVRLAQLWANLLSNAIKYAKPGEPPVISIGCQTLEEQLIFSV
                     RDKGIGISPEFHQKIFGVFNRLHTEDQIEGTGIGLAIVKRIVEFHKGKIWVDSAEGLG
                     STFFFTLPKNHEGYGKSLSAGIANSPPATPTRL"
     gene            complement(23599..23985)
                     /locus_tag="VLA60_08085"
     CDS             complement(23599..23985)
                     /locus_tag="VLA60_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF09358.1"
                     /translation="MVTEHPNFVWRQWGSIALIGLTLWLMPGVKVQVVHGQDTSVEKG
                     KGLYQRLCSACHGKAGKEEAYPFFDPPPADLKSPATQNKTDEALLETIRNGHPNTAMG
                     KWEYALSEEEMKEVLAYIRTLGGTTS"
     gene            complement(24023..24661)
                     /locus_tag="VLA60_08090"
     CDS             complement(24023..24661)
                     /locus_tag="VLA60_08090"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HSF09359.1"
                     /translation="MKAMFLLAAICLSTALSACALSRGTDESMRSYVLEIEKGGETRA
                     SDEFRPSNLPSLLVSLPQPAPGYESQRMAYQQVPYELRYFATSQWVDSPARMLAPIIT
                     NALESSGEWGAVIQLPSVLRGDYRLDLSQVALVQEFTQQPSRIRLALRAQLTTVFAPR
                     VIGTRSFEFHEASSSEDAYGGVHAAQKVVGKLLVELQHWLQGCLHTVQPSDC"
     gene            complement(24666..25631)
                     /locus_tag="VLA60_08095"
     CDS             complement(24666..25631)
                     /locus_tag="VLA60_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HSF09360.1"
                     /translation="MMEPRGNYLIVGVFVFVLGVVAVGIVLWLGKSDYRGVYDQYHAY
                     MRQSVSGLSVNSTVKYRGVNVGRVKEIALNPDNPEEVRLTLDILQGTPIKTDTVATLE
                     TQGLTGLATMNLEGGSREAPRLEPEPGQEYPVIQTKPSLFFRLDMAISRLLSEQGVSR
                     LLNSLHGLSENAATFMNEENRLKMEGIVKDLATVSHTLGRQHETIAQGIDRAFEAAEN
                     LAILTSTVNQDMPRLVARIHQSVTAVKTVAEELARTGKTLEAVVQESRPDIQQFTRET
                     LGETGQLVTELRQLTNTLQRVAHQIEQEPDSLIYGRPSQPRGPGE"
     gene            complement(25628..>25867)
                     /locus_tag="VLA60_08100"
     CDS             complement(25628..>25867)
                     /locus_tag="VLA60_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09361.1"
                     /translation="AGFDELVPQLKAVLGLTVVMVTHDLDSLWRIADRVAVLGNGTIL
                     RVGTMQELAESRDPIIHEYFHGPRGRAAQSQYEAS"
BASE COUNT         6307 a         7157 c         6313 g         6090 t
ORIGIN      
        1 ccaagccggc caggccgcca aagtgcccgg tctctgtcag ctcctggccc cgcatcgtca
       61 cagcgccagc aagccgcttc gcatgctgat catgggcatc cccaacgtcg gcaaatccac
      121 gctcatgaat agcctgctca aacgccgcct tgctaaagtc ggcaacgaac ccgccgtaac
      181 gaaagtccag caacgccaca ccctgaacga ccacatgacg ctcatcgact cacccggcct
      241 cctgtggcca aaaatcgaag accccctggt gggcctcatg ctcgcgacca tccatgcagt
      301 caccgccaag gtgatggtcg aggaagaaat cgccgagttt cttgtcacca tcctcctcgc
      361 gcgctaccct actttgctga cgaaacgcta cggcttcctc acagagggtc tggatagcac
      421 gggcgtgctc gaagccatcg ccacaaaacg cggctgtctg cggaaaggaa aaggcggaga
      481 attggatcgg gaaaaagcgg cgaaaattct cttgacggaa ttccgcaatg gcacgctggg
      541 ccggattagc ctggagacgc cggacacgcg agacgctcgg ctctaatgtc atacttcgtt
      601 acgggcttag gggtgtaatt cctgttcgta ctacgcaccc tgcttcgctc agaacagatt
      661 cttcgaagac tcctccgggt ccagcaattg tgaaacccct gtttttttca gcatcacctc
      721 taggtttctt cgttgccttc caggcatttg atacattcat ccatttgcgg gtcaccatga
      781 agacgacaaa aatgccggga gcattgtata cacaccaaat aacttagact ttccgaccct
      841 gcttccttac acaaatagca gggcttttcc cttttcgcat ttggcatatc tttcctcctg
      901 caatgggatg gggataagca tatctccaga cactcgagat acccgctact ctaccgagaa
      961 gactggcatc ccaccaaaga tcagagtgag tcatatccca aaaaaaatat ggagggcatg
     1021 actgaatgca ggttcggagt gaaatagcat catgatagga ttggacagtc cggagtctca
     1081 tatcgccgaa ggcagattgg ctgagcctaa gtggtctcct gtgacctgca tgaaaatcgg
     1141 cattcaacat gattggccca ggcggaatca gaggaaaaat ccatacagga tcgcggacga
     1201 acacctcgac ctaactttcc atcatctcca agcgggtatc aaactcatgg ccgcaggaca
     1261 cgcaccggat acaatcagat tcaactaccg gttcatcatc ccggataccc ataccaccac
     1321 aatccggaca tcgtgccaga tgcaacactt cgtccttgac gttttcatag cgggtaccac
     1381 gtaagcaaaa aatcggttgg ccatcgagca accacggttg acagtcttta ttgaccacag
     1441 ggagcacaag ataatgatga gcagcataat cctggatcga ctccggggta ggaaacccat
     1501 ccttaatcaa attcccggtt ttcgcatcat agatcccaca gtcttttacg acagctttcg
     1561 ttggccccat acgatacctc cttctggcag gatgaatctg ttcgtcaacg gtgatcaccg
     1621 acaattatgc tctaaaggcc tgctgaaaga ttatgagggg aaatggccta gctgcacaac
     1681 cacgagcatg caatgaaaaa cgggtacgca atggaaggcg atctaggcct cacccttaga
     1741 gatcagaaaa atcacgcatt gcccggctcg atgccaaata agttgaagag caggtcaccc
     1801 tcgcttcttc attttgggtt tgccccacgt ttcactcaag accgatttcc actcatcgcg
     1861 ggatacggcc ggcatcgtct taatgcgaat atgtcccatt gaggcattga gcacaccggc
     1921 cttcatggcg gcacgattat tgggaaactc taaaatggcc accacatcat attcccccag
     1981 gcaataaaag gcgtgcagca ttttcccgcc caaggactcg acatttttct tcaccatgtc
     2041 cgaccgctgc acgcccttgg ccttcatggt catcagcccg tcatgggtaa aattcactaa
     2101 actcacatag atcggcatga tatacctccc tgggaaaaag tgaataatga aaaagatgtt
     2161 ttcctagaga cgagatacaa ctatcctaat ggaaagaggc aataaaaaat agccttgctc
     2221 tgattacctg gcgatgacca cgcgtgactt ttgggcatat gatggcatcg tgtgtgcgaa
     2281 aaacggcatt gaatccaaaa gggttccatc cttaaacatc atgagccggg aggaagctcc
     2341 tcaatggcat ccatcttctc tggataaaag gccacgtaat ccttgatgtc ggccacagaa
     2401 ggtaagggag tctcatacgt ccaaaccgca ttctcggccg ttttgccctt cacgctcacg
     2461 ctgaaatacg acgcatcccc cttaaaaggg cagtgcgtag aatgagtcgt ggggcgaagg
     2521 caggacatcg tcacatcctc acgcgggatg tattgagccg gtggcacagg gccctcccgc
     2581 aatgtcagcg ctcggctggt gtccgcaacg acggttccgt taaacgtcac cttaacccga
     2641 ttgggattgg gcataatggt aatggcatga ggatggctcg atgcagtcat ggtgatcatt
     2701 cctttcctga gtttaaaaat tccggcggaa gcaacagcgg tgaaaggagg tggcagacaa
     2761 tctctgatgt taaaaaaaac atggtccggt ttcctactga agctgttgag caacatccag
     2821 gaatgtctga agattagcgg ggctgctaaa ccgatgatcc ccggtcttca aaagtagttc
     2881 gataggatat tgaggatctt gtgtgagcag gtgttcggta aattcccagc ttccttttgg
     2941 aaggattacc acgtcctgaa gcccatgcac aatgcgcgtg gacaccctct tgggtgaatc
     3001 caaccgcctg gcccataagg tctcgctttc ttccacccat ttccacgaga gcggaaaatt
     3061 tttatgcaag gcgtcatcgt cccagggcat ccatccggtc tcctgccacc gttggcgtcg
     3121 ttcaggcgaa atatcctgcg cgcgttttcc catcatatta aacgccggag ccaggagcac
     3181 caattgttgc acgtgaggaa actcttggcc caggagccat gccagccagc cacctaacga
     3241 agatccgaca atggtcacag gcggaccatc ggccagcatc gttgtcacgc atcgcgcatc
     3301 tgcaagccag tcgaacaacg tatagtcggc gaacgcgcct tctgattcgc catttccacg
     3361 aaaatcaaaa cagcaaaatc cccagtctcg ctctttacac aaatcggcca gagccttgct
     3421 tttattgccc caccgtttgg aaagaaatcc ggtcacaaaa agtaattgtc gttcctgccc
     3481 ggaaaggcgg tcaccgacaa tgcgtttcga gtccgctccg ggtatttcaa atcgggttga
     3541 ttgattcatc atgttccatg ggctgtcagg tgaataagaa attaacgtgt tcttaaagaa
     3601 cgtgaacgga aagatctaca gaaggagtgg aaaccatcat cgtgcctgtg cgcagcgaga
     3661 aggctcgctg caagtgaagg gagacgtgcc attccagtgc ggaaccaccg gagtgattcc
     3721 caaagcgcac gccccttcca tggcctgccc gatatgatcg accaaaacaa agtccacccc
     3781 tcgggcgaac agatccccca ggtcttgcct gagctcgctc tcgtcttcat ccccgagcca
     3841 cgaatagttc acacggattc ctgcctgatt cagttgatcc atggtctcga cgctgggtcg
     3901 cccacgaaaa aaggacagct ggataaattc gtcacaggaa ttaatggtgg tgtcaacgta
     3961 ctgggagcga aacatctccc ggctcatgtt ggcgacccag ctctcttcgt cggcctcccc
     4021 tgccacacgg cgtacggccg gatcggtatc gtcacgcgcg ccgaagataa tctggttgaa
     4081 gcgttgtgcg ttggcgacca tctcggcagc gagccttcca accaggggat tgttctcttt
     4141 gatatccaca ttgatccaga tattgtgcgg cataacggca agagcctctt cgaatgtggg
     4201 aatacgctgt cctgccacct cgcctgcaca cgcctcgagc tgcaggctct ggacttcggc
     4261 gagagtcgac tcggaaatgc gtagcgttcg cccctgctcg tctgtcacgc gatcatcatg
     4321 tgaaacaaca agtacggcat ccgcagtcga tcgcacatcc agttcaatct ggtgaacacc
     4381 aaggcagatt gcccgctgaa acgcaccgtt cgtgttcacc ggacatccta actgcccccc
     4441 gcggtgagcg gagatccccc gctcaggaag gtcagttttc tgcaagtgag agacctcaca
     4501 ggaaaccgga gccacttcgg acggcgacga agcacatccc aagcagagtg tcatgccggc
     4561 gagtgcagct ccgtagaatc gccaggtggc ttgcagggac ttcgcgcggg accaggagca
     4621 tggcatcaca caccccggga cggccgatca ttttcaagag atctgttgag agaggtcacg
     4681 gttgctccag catgagttca ggcaccccgc ccatcgtagt caccagcaac gcgagcagga
     4741 tggcctgaat aatccagccg atcagacaca ccccaatggc acggtaggtg ctctgataat
     4801 ccaaggcttg gcgcaccgca atgaccatgg ccacgagcat ccatacaccg gccaaaagat
     4861 tcacaaaccc gctcagggcc gggaaaatgc cgaacacacg aatgaggcca ggggcactgg
     4921 aaaagccgat cgtccgtaag agttcaccat gatccgcgtg ggtttgcggt tcaggaagaa
     4981 gtttcgtgcc aatgaggtag gtcatgaacg cccacacata ccatccgagc agggacccga
     5041 ccgtaccaat gaccagtccc atcagaccgg cctcctgcat aaacccgata ccaccggcca
     5101 ggctggataa cagcacgacg cccatcgcct ggcccatcga acgcctatcc gcttccacct
     5161 cttcatagag gtgaacatcg agtttagccg cgcgtatcat ccgatcaaca aacagactca
     5221 tatggattat ttcctttccc tgacttggga taaatgtcat tacaaccgaa atagattgtg
     5281 cccgaggagg aagaccacaa gcaattgcgg gaaaaaattg tggtttcctc tttgctcaaa
     5341 ggggtcagga aaattcaatt cccacttcaa tatctgatgt cactttttga aaatggcggg
     5401 aaataggcta tctgataaaa attctggagg agaaatacct ctaagaaggg agcaaatgct
     5461 cattcatgaa ccaggagatg agccagaaat ttcgttggtg tcaagcaaca cccccttgtt
     5521 ttcaaagggc aagcgggctc cttgtacata taggataccg tcgacctggt aggagacttc
     5581 ctgccgttgt gagagttgaa ggagttgtcg tatgacatcg agtgtggagg tcgtggtctc
     5641 cacggaaatg acggcatccc ccagtctcgg aatagtcgag gccttatttg tcaggccacg
     5701 ggcaagtcgt tgatcattaa ggtgaagggt gaaatcaaga ccggtgacct ccagatcaaa
     5761 atcattggga ttccgaacgc gcaagtcgac ttgcaaacgt tgttcaaaca tcgtggcatc
     5821 taacggagtg atattgacca gcagaacttc cggcgattca ggctggacag acaaggctgg
     5881 aagagaagtg cacccgataa gaaaaagaat tccaaacacg aaagggagtg ccaaagaaaa
     5941 ccgtttcatc atgatgaaga atcatacctc aatctgaaag gcaaagcgat accaagggga
     6001 ccagcatgtt agcgtcgtaa ggggcatcct ccaagcagtc gccagtctta ttttctcagt
     6061 tcttcgatac ctagatacat ttccgctaaa aaatagcggg catgattcag agagaggcga
     6121 tggattcccg attggcaatg tcggaaatga cccctgcgga ttttattgct ggaccgcttg
     6181 ttgaatgaac tgtttcgctt cgttcacctc ggggagatcc gaatcagcct gattccatat
     6241 gtttagaaaa ttgctataga cttgccgagt cgcctggata tggcccaatt gagctgccgc
     6301 ccgtgccagg cctagaagtg aacgtgcccg attgggatgc cataagagcg atcgttcaaa
     6361 ttgttctttt gcctctttcg gccggtcgat gcccaacagt aactcgccat acagttcatg
     6421 ggagggtttg atgagatcag gtgggccgga aggaggcgga agggattctt ccagagcggt
     6481 agcctgatca agcatccgta gggcctgtga gtggtctccg ttgataagat gtttcaatgc
     6541 tgcgatttgc agcttccaga tttgtaaagt gatcggttcg atcgattccg tggaaggctc
     6601 ctggactggc gagtttattc cggggtgcac ccggtgagcg attgattgat ttctcaatgt
     6661 tgccatgctc tgcgcgaatt cgcggatata gacggatatg gatggcgact cgtcaccaaa
     6721 gcgatagtca ttcacgttcc aggcctgatc cgctaggtcc catcgttcgg tttctataat
     6781 aaaggcggcc accatctgat cgtaattccg gttgaccttg cggtcatagc caaggacctg
     6841 tgtctgttcg ccggatgacg attgcgccat atcctgctgc ttctccaata tcagggctgc
     6901 agcggcgttg tatcggcctt gttggagata ggcgtattgc aaccagtaca agctgtgata
     6961 atcctgaagc gataagggca actgtttgtc cttgacccag gcctgcgaac ttttccatcc
     7021 ggctttgttt gaggccgctg cctggtccca catgcccagt tgaacaaata tatgggcggg
     7081 catatgttgc gcatgatgcg cttcgggcgc gatcttggca taacgacggg cttgagggag
     7141 ggccaggatg gcatggatcg gatcgtcaaa ggcatgaatg gtgtaatgcg cggcgcaggg
     7201 atgattggga ttcactccat acacccctaa ggccagggcc ccggcctgga cgcgatattt
     7261 ctcctgaaga tatggcttct catcatagtg gacggctaac ccaaggaggg acagggcata
     7321 caagcaggtg gcttccagat cttcgggata ctcgctggca aggctttcca ttcgggccgc
     7381 ataggcctga tcgcgggcaa gcttgtctcc ctccccaaat aatgtattca cggcctggag
     7441 atactgacgc tctctgaccg ttgccgacgc cgtatcggaa actttcccca gcgccgcccg
     7501 tgccgcctca acattttctt cctgccagac gggatgatta ttcgccatcg cttcacccca
     7561 atatcccatc acaaaatttg gatcaatcgt ggtcgctcga cgaaaagcgt cgatggcttc
     7621 ctcataccag aacgaatgca gggccgcgac gccccttaag aaatggggat gggcttccct
     7681 ggaccctgat gcgggaaatt caatacttcc caattgacct aatgccgcat tcccctccga
     7741 tgaagaggaa gtatgatcca tctcgccaag gcttacctgt ggaagtgcca tgacgaagat
     7801 ggcgatgagg aagtaccgga gacaggatcg tcctggcgca tatttcgacg aataaagctt
     7861 gatggacatg ccaactcctt gattaatgac ggcggcccgt cattgctctc ttaatgatgc
     7921 tgtttcactt tatcattttt gttcaaaaaa agaataagac aataataaat gataggaatg
     7981 agtgttcatg atttgaactc acactcaaga ttgtcattcc gaacaaagtg aggaatcctg
     8041 aaatccgaga cttaaatcct tccattgtga attttcgccg tttactaagg agtccttttt
     8101 ctctctcctc cattttttat ttctttttct cgcatgatgg ctgcattcac ttcttgagtt
     8161 tttcaaaata cacgagtttg tttatgttgt atttttgggt aaacctgttg tatttttggg
     8221 taaacccttt cacaagtttc ttcttatgtt cgtacattct gcgttctaag ttgttggtca
     8281 ttcctgcata catgacattg ttgttccaat tggtcagaag atagacatcg taggtatgtg
     8341 ccatagaaag gatttctccc tctcgtcgaa atgacagcaa ggccaggaag ggaccttgct
     8401 agggaacctt ggcgcgaccg atggtgttgg catccgtgcc gaaccagatg gttttagttg
     8461 gttggtgata gaccatatga cgaacgcttc cgccaccgct ggggatatcg gtcagactca
     8521 tgaacgtgtg ggttcgtgta tcgaatccga cgaggcgatt ggggtgcgcg ccggtttcca
     8581 caaaccacac ccggtcctgg tcatcgacgg tcatcccgta tggtttggcg tcggcttctc
     8641 ccggcgccaa ccattcctga atgtcgcctg ttttggcgtt gtaagatcca agatacccct
     8701 ccgcataatc cacataccaa atgacaccat ccgaggtcac cgccagtcgt cgtggtcgtg
     8761 tttcggtccg tgagagggca aattcctgaa cggtcattgt cgcccgatct accgtcccca
     8821 acttgttggt accaaattcc gtaaaccatg gggagtgggt gggatccagg accatgccat
     8881 aggggcgagc ctgcgcagtt ggtagcggca tgagttgaat ctttccggtg gccatatcga
     8941 gatgccccac gaaattccct tgttgtaccg taaaccagat gtcccgatcg tggccaaaca
     9001 ccagcgtatg cggatcctgg gcttttggat ccggcatggg atatgtaatg atcttgccgg
     9061 tggatggatc aagtttgccg atgtgggcgg cgcgatttcc ggcataccag acctgacctt
     9121 cctgatccac aatcagattg tgcggaccgg tgccgggctc cagtttgaat tggtgaaact
     9181 gcccgagctc agggtcaagc acggcaatgt aatcacctcc ctgccccacg aaccacacgc
     9241 gttgctgttg atccacatac ggatcacgcg gtctcgtctg gtcccaaggc acctgccatt
     9301 ctttcatctc caccgacttg agggaggcag cttcggcttt cgtgggatga ttggtgcatc
     9361 cggaaaaaac cagtgggatg atcagcccaa tgatgatggt gcttgtttgg tgtagacgat
     9421 acacaatggt tctcctctta gaatcatcat ggatgacgct ttccacaaac tctactccat
     9481 atgttggcat gtctggaaat attaggcaat caacgatcga ccgggcagac gggatggacg
     9541 tgataggtca gggaaggaaa acggatgccg ggtctccggc aaaccatttc ttttttaacc
     9601 aaagcgcgac atggacgaga gctaagagta ccgggacttc aactaaaggt ccgatgacgg
     9661 tggcaaacgc gacagacgat cccaggccga atgcggcgat ggccacggcg atggccagtt
     9721 caaagttatt gctggctgcc gtgaaggaaa tggccgtggt tttgccataa tcggccccga
     9781 tgagtttgcc catgctaaag ctgacgagaa acatgatgac gaagtagaga atgagtggca
     9841 gggcaatgcg aaccacgtct ccaggcagac tgagcaccgt gccgcctttg agcgtaaaca
     9901 tggcaaagat agtgaacaga agtgccatca gcgtgagagg accgattttc ggaagaaatt
     9961 cctgttcata ccacacccgg cctttttgcc tgatcccgat ccagcgtgag aggaacccga
    10021 acacaaaggg gatgcccaga tagatgaaga cactttcggc aatcgtctga aagctgacgt
    10081 cgacgacttc cacagcaaga cctaataacg ccggcagaat gtccaaatag aaccaggcca
    10141 atccactaaa gaagatcaat tgaaagaggc tgttgaaggc caccagggct gccacatatt
    10201 ggttgtcacc ttcggcgagt tgattccaga ccagcaccat ggcaatgcag cgggccaagc
    10261 caatgaggat gagcccggtc agatattccg gcgaatcgtg gaggaatgtg acagccaggc
    10321 caaacatgag caaggggccg atgacccagt tttggacgag tgacaggagt agaattttcc
    10381 aatcacgaaa gatcaagggc agctcttcat acttcacacg agcaaggggg ggatacatca
    10441 tgagcatgag gccaatggcg atagggatat tggtggttcc gagagaaagg ctgttcacca
    10501 atgccgggac cccggtgaag accgagccga tcgtgacgcc ggccgccatg gccaggaaga
    10561 tccaccccgt gagatagcgg tcgagaaatg ataaacgccg tggggcggac acggtcatga
    10621 cagtacctct tcgattcttg acttgagcag gaaaaaggtt cggtcaaagg ccgcgttgat
    10681 ctggtcttct gtgcctatca ctctcccggg atcgggaatg ctccaatgca gctttcgggg
    10741 ttggccggaa aagatcgggc aggcttctcc tgcagcttga tcacagaccg tgatgaccag
    10801 gtcaaaaggc tgtgaaatca tgtcatccca cgatttactg tacggctggc ccggatcgat
    10861 cccatgtcgt ttcaaggtcg agatggaacc cggatgaacg tatccggccg gatgactgcc
    10921 tgcactccag gcccgatatc gtccttggcc taggtggtta atgagggctt cagccatgat
    10981 ggaacggcac gaattacctg tacaaagaac caggactttt attagctcaa tagacattag
    11041 ctacagcagc cgttggattc ctgtaccggc atgttcgtct tggcactcgc cgggaacgcc
    11101 ggttcgcaac aggcggtttc ggtgtgcgcc gaatgggttg aaaagatctc cgcgtcttcc
    11161 atcgtgcggt aggcttccca cgggactccg gcaggatcca cgacccagga tttatccgat
    11221 ttggcataac aacacagagt ctcgccttcc tcggccaccg ggagattccc gacttgaagt
    11281 cgttgtctga tctcgtccaa ctcctgtccc tcctcaacct gaatacccag gtgatccacg
    11341 cctttcgtgg atgtccgcgt ggagatggcc aggttgacac ggggatcttc gagcatccat
    11401 ttggcatagt ccggtttacg tttaaccggt tccgccccaa acaatttgct gtaaaacacg
    11461 atggcttctt ccaactgttc gactccgata tgaatatgaa gacgtttcat ggatgtgcct
    11521 ccttggtgag tgggttagca acaattcgag agttcgataa tgctgcactt ctttttttta
    11581 ttgtcccgga tactggccat atcttcgcgg cagcagtctt tgaccatata gcgaatcagg
    11641 tcggcaaaaa attcaaagtt ggcgctgtag atgatggacc ggccttcacg tctggaaacc
    11701 accaagcccg ccttgctcat gtgcgagagg tggaaggata gggtgttatg gggaatgccc
    11761 agagcttcac tcaatgttcc ggcaggagcc cctgccggac catattccac cagcagacga
    11821 aacactcgta accgggtatc ttgtgagagg gccgcaaaag ccagaagagc atcattaatt
    11881 tccatatgtc cagaataatg gacatgattt aaaaaaagca aggcccctga atttatcagg
    11941 ctggggatgg atcttgggat gcgttaaacc agttgtcggg aaaataacaa tacgggtcaa
    12001 cactcagagg gaaggactga gtgaattttt catccacggc tcaaatcata gaggccatag
    12061 gtgtctggcc tttttgagac aaaaattaaa gaggtcgtgg cgaagcagac taagggcata
    12121 acagttgggc gaccatggag cgcaactccc caaaatcata cggcttgcca aggatggcat
    12181 acactcctaa gcggatggct ttctcacgga tttcagcgtt gagcaaccct gtgaccataa
    12241 tgaccgggac atacgggctt tcgggtctga gttgtaagcg gttcaaaaac tccatgccgg
    12301 tcatgacggg catggcatta tcaagaataa taatgtcaaa aggctgcgat tcaattagag
    12361 acaaccccgc agccccattc tccgcctcca cgcaatggca tccatcatat tccaggacca
    12421 gcctgagcaa tctgcgactc atctcatcat catcaacaat cagtgtattt ttggacatca
    12481 gggcatcctc tcctggtaca cagccaaatc caccaccctg atagacagac tcgactgaat
    12541 gccggctcac cgttttgggc ctgttgaata atagggggat tagtcgagct ttctgctatc
    12601 ctcttaccat atgacctccc catccacctt ctcccatgga gtctttcgta tcccaactca
    12661 aaatggtgac tctcgatagt ccataaccgt ccacccattc ccatcgccct ccaattcatg
    12721 aaagactttt ccgattcgtt aaccctgcgc tatttgtaaa aaacccgggc cgcctatggg
    12781 cttggggctt gaccttgtcc aggcctaggc cggacgacag ccttatcacc cctgtctaca
    12841 catgtattaa ccctctcacc cgtttaccac gatgctgaat ggggctgttg aaaaaaaggc
    12901 gccagcggcg ttccctgcct tcgccggagc ggcttcgcgc aggcaggtcg ccatttttcc
    12961 gtgctcacgt actggaagta cgctctgcgc gcaaaaatga ctgcgccctt gctggacaaa
    13021 cttttttgaa ccgccccgag gccactaata tgcaacgtgc gtgcgggtca atatgccctt
    13081 ggaattggtt ttattcaaca ctccctgaat ccgctcaacc ctgccaggtc gatgagtaca
    13141 gtgttcatct ttgagggatg ttacgcatat gatagtcaag gctactctgg gggaagagca
    13201 ggatagagcc ctaccataga agttagacga gctgaacacg gagattattc ccaaggtggg
    13261 ataaccatac aagcccggct tgggtgcaag tgcaagaaga aatgatggaa cgactggaag
    13321 gaacatgtca gtgcgagcag acaatccgga ctcatcaaca agatggtcac catgtcgacc
    13381 tatgcatgtc atccagggca atgaaccgga tacccaaggg atctccgtga gaagggaagc
    13441 cgcttcatca acccgggcat tcaaccgggt cacactccca aaaccccttc ccacggatag
    13501 gttatccgct attcttcaac acgccctgtt taccctggag tcggatttct ctctatcaga
    13561 ccattcattt ttccccacgc cattcaaagt caagacctct tttgccgcct gaaaccagtc
    13621 cgcttgatca ttgccatgtt gcccccctcg ttgttcaaac aattcatacg agcgcttggc
    13681 aattcgttcc tgcaattccc attctgaaag accaacagat tgttctgtcg ggaagtgaat
    13741 ggtgcctgcg ccattctgcg gttcaaccgg ttgggccaaa gaatgtgatt tccctttatt
    13801 cccggatttg ggtttagtcg tgcgaaccac catcttggat ttgggaagtc gggatgccga
    13861 tccctttgaa ggactctctt cattgtcaga tttttgagat ctcataactc tcctttatgt
    13921 gtgtatgctc gccataagtt caccatgaat ttctatttcg cccgccaaca ctttgttggc
    13981 ttagaccggc cgggtccgga tctctacgct gttgaatccc acggtcacag tacccgtttg
    14041 acccacctta ccaccgagga ccggagccac tgcctgtgcg agccatccga ggcttcgcct
    14101 cattcacgag caaggtcctc ccctcataca ccttatcatt ccaagctgca atcgctgcct
    14161 ggacctcctc ctccctggac atctccacaa agccaaagcc acgagacctt cctgtcaatt
    14221 tgtccatgat cacctgagcg gactttacca cgccgtaagg agcgaacagc tcgcgtaatt
    14281 gagcgtccgt catagcaaac ggcagtccac ccacaaacat tttcaaattc atacgattcc
    14341 tcctaacagg ttgcggttag agcaattgac atcacgtgag gaaagaaaac ggggaatgcc
    14401 aaggccaaag gcgtgatcac acgtgcgaat gagatccact tccaatccca tgttttctga
    14461 caaaatcaaa accctcatta tgcgacaagg attctttttg cttgatagag accggcctgc
    14521 ccggcaccgt tcttttcccg gaccggtgtt ttctccaggg acgttcgata agaaaaatgc
    14581 cgaagtgatt tctgcagcaa gagataggga ttgaatggtt tcaccaaaaa atcctgggca
    14641 ccctcatgaa gtaaatcccg gagtttcttt tgattagcct cttgggacat gacaatgacc
    14701 ggaacgtcgt agccctgcca tcgcagttca tccagcatgg tccacccatt catgaccggc
    14761 atctccaaat caagcaaaat gccatcgaat ggcatagtac caagcacagt gagaccttca
    14821 cgcccattgg aggcagtcac cacgtcaaat cccaaccagg ctatcgcatc ggctatgatt
    14881 cgcgcactgc ctgcctcgtc atcaacaacc agaatccgct cagacatcat catgacctcc
    14941 attcaagatt cgattcaccg agttttttgc attggagctc atcatgtcgt gataaccggc
    15001 atgaactccc tgccgcacga ttttcgccaa attcgctctg acccctgtcc caagggtgac
    15061 atcggcaaaa cgggccttca cgtttgtcca aatttcgcaa gagtggacag cgacagcgtt
    15121 actccgtcac ggaatcagac cattaccctg cgctcgaacg atcatgttcc cgctctgcca
    15181 tgtcgttaaa ttaaaaaccg cttgggaacc tttcccccac caatgtctga cggaatggca
    15241 tgggaatagt ctcttgattt ccgctgatta tcaggcagcg cctctttttt cattggtacg
    15301 gacttgcctg ttcctggtga ttccagttga ttcatcctac tcccttctct ccaaatagcg
    15361 gtcgatgtgc ttcaggtaga aaagcctctt gctttcaaca tgtactggca taccagaatg
    15421 ccaagcccca tcatgtctgg agggcggttg ccggttccat gtccaatcgg cgaggcttcc
    15481 tcatttggcc cgctctttcc aaggaaaaat tcggctgtag tccaaagcgg tgaatatcat
    15541 ccaggatacg ctcaggactt ccccaatcac tccatgacac cccagccatt tcgagcatcg
    15601 cgaggtgatt tgtcacacgc gccagcaggt gtgaagagaa attccacgca ggcatcacgc
    15661 gataaatcga ttccagcacg gttccttcgt acgaggtgcc aatcgcatgg cccagtcttt
    15721 caaacagcgg catcatttcc gggaagcact gccatcccag tttccacaat gtttccgcct
    15781 tggccgctac caccatcgtg ttccaccaca tgcccgaccg gctttgggtc ccccattcgg
    15841 tgagatccgg cttttcgagg aatgaatgaa ccgctcgaat cttttgacca gagacccaac
    15901 cgatttcccg cccgacctca atccatccat aatccggctc tgtcctgtca ggcttcactc
    15961 cgagcatcac gagtcggtcc gtcaaccatt ctgttgccga aaccgctcgc cccacgattt
    16021 ccaaaaatcg tgcctcggga gtcacaaaat gatccgacgg ataaatcacc accgtggctt
    16081 tggcatcacg tgctcgcaca taggtcaacg ggagaaagat tcccggcacc gtattacgat
    16141 tgagaggttg actgatgatg gtaccggctg cccggtctgc catttgctcc cacgccaggt
    16201 gttgatgagc ttgcgaaatc acggtcaccc ggcgttccga aggtaccagg cgatcggccc
    16261 ggtctatggt atgttgcagc atggaccggg tcccgacaaa cgtgcaatac tgcttcggct
    16321 tgtgatcccc aaaccagtga caggtggcaa gtccaaggcg ctccccgttg cctcccgcta
    16381 aaataatcga ccaaacatgg tcattctgct ttcccgaacc catcataaac atcctccttc
    16441 attggcctca atgacggtgc caagttcaat ccgacacttt tgtgataggc ctgacacatc
    16501 catcattctt cccatgctct ctcaactgac tcgacccaga gggagagacg catgagacgg
    16561 aatgggcgtt tttggttggg tgtggcttcc gaactgacga ttggcaagaa tctgcgaatc
    16621 aatcacttcc ccgcaattca cacatttcca tatcccggcc ggatcatctc gacataccgc
    16681 ttcaccttcc acaaacgtct cgatcatcca tccaccacat cggaaacact gtcctctcat
    16741 gcatcactcc ttcatgattt tgctactgtg gatgaaggta tagcactccg atgatagggc
    16801 cacaatcacc aatacggcga ttttcccttc acatttctga cgattgcgtt gtcgtggttt
    16861 gtactacaga ggcagggatg accgaaccgg tttcccggga gaaaaagatg ccctgtcgga
    16921 atatcccttc aggggatcaa ctgatgctga aaggcgtacc gggttatttc cgcattgttc
    16981 ttcattttca gtttctgaag aatacgtgag cggtacgtac tgacggtgct gacgcccaaa
    17041 gacagttcct gggcaatatc ccgtgcggtc ttgcctgaag caatcagact gaggatctga
    17101 tactcccggt cggacaatgt gagatgagga gggcttccga ggtcatcgga gagggacatg
    17161 gctaagactt ccgcgagtgc ggaactgata tatttccccc caagcaaaac ttttcgaata
    17221 gccgtcacca attcttctgc cgcactctcc ttcgtgaggt aaccggcggc tcctgctttt
    17281 aagacacgca cggcgaattg atcttccgga tgcatactca acaccagcac cggaagcttg
    17341 ggatgcaatt gcttcagatc cttgagggca tccaagccgc ttcggcccgg catggtcata
    17401 tccaggatta caatgtccca ttttcgcttc ctgacgaggt caaggacctc atggatattg
    17461 gaggcctcac caacaaccgc atccttgaaa tcctcggcga gaatttgctt caccccctgt
    17521 cgaaccacag cgtgatcgtc tgcaaccaga atttttacca tactcctcct caaaacatgg
    17581 gattccccgt cgtcacttcg agagtgccga cgggccagcc acctgacaga ccattgtgaa
    17641 tcatccctca cggttgagcg gcaattgcag gaagaccgtc gtgcctttct ctggttcacc
    17701 gacgatcgtc acctccccac cccaaagcag ggcgcgctcg cgcatgccga ccaggcccag
    17761 ggatttgggg ctggaaatct gcatgagggt aatgccgcaa ccattgtccg tgaccttcaa
    17821 ttccagatac ctcaccgatc gttttatata tatgctgacg cttgtcgcat cggcatgccg
    17881 ggtcacattc gttaagatct cctggaggat tcgaaagacc gttgtggcgc gctcactgtc
    17941 gataaccaga ctctcaggat aaatatccaa ggtacaggta atcccggtcc gacactgaaa
    18001 ttctttggcc tgccattcga tggccgggcc aagtccgagc tcatccaaca ccgctggtcg
    18061 caattcggta ctgacctttt gaaccaattg gaccatctca ttgacaaatt ttgccatgga
    18121 atgaattttg ggggccagtt gagaagccgt ctcccttgaa ttcgtcatgg ggattcgctt
    18181 ggccatccag gccaggtcca tttttaagca ggtcagcgcc gaacccagct catcgtgaat
    18241 ttcgcgggct acccgggttc gctcctcttc ccggaccgtc tccaaatgcg cagccagatc
    18301 gcgcaactgt tcatgagatt ccttcaaacg ctcatctgat ctttttcgtt cggtgatatc
    18361 acggaatgtg accaccgctc ccacaatcct gtcgttttcc cgaatgggat tgctggtata
    18421 ttccacccag aacggcgagc catccttttt ccaaaacaca tcctccggga catgataggg
    18481 tttcccatcc cgaaaggctt gatgaatctg acattcctca accgggaaag gcgtgccatc
    18541 ggatttactg tggtggagga tatcgtgcat ggactggccg tgtaattctt ctctggtaca
    18601 gccgatcatg ctcgcggcgg ccggattcac caatatggtc tttccctcca gatcaatccc
    18661 ataaattcca tcgtgggctg aattcaacag gagttcatac ttgtagcgaa gtcgcgcgag
    18721 tgaggcttcc gccttcttgc gttccgtgac atcccgcgcg attcccactg tagccgaaaa
    18781 ctgcttgcga tgatcgtaga tgaccgtcgc atccacctca atctcacgga ttccaccact
    18841 tttcatcagg aagcgtatgc ttgatccacg ggttgcctga cgttccatgc cttctttgtg
    18901 aaaccagtgc gcagagcgag gcagatcgtc gggatggagg aaatgtgaga caggaacccc
    18961 cttcatttct tccggggcat acccaaggat gggaagcacg gccgggctga caaagcgaaa
    19021 gcagcccaac gcatccagtt cgaacacgat gtcggtgata tgttcaatca gtcgatgata
    19081 gcgctcctcc gtatccttga tcctaagccg caaatggcgc ttttcaagca cttcacgcac
    19141 caccataggc aattccagca agaagttcgt ggattttcgg aggtaataat cggcgccggc
    19201 gagcatcacc tggactgctg tcgcctcatc accatgtccc gtgagaagaa tgatgctgga
    19261 ttcgggacag agagacctca actccttgag aatctccaac ccgctcttcc ctggaagctt
    19321 gtaatctaaa agaatcaagg accaactcga tgctctggcc ttcgccaaac ctgcttccgc
    19381 cgtcccggct cctgcgatga tgctgcccgg ccagacttgc agaagagctt caccggtcaa
    19441 ttccacatca tcgggttcgt cgtcgatgat caaaattctt tgtgtgtctt tattaggcat
    19501 acgcatctat gcacattcag tcaggcatca tattatgagt cgaggcacac cgtgccttga
    19561 ctcccgccct ctcacaccct gtttcgtaaa cacaggaaat ccgtgccctt caacgtttca
    19621 gcgtttcccc acttggaagg gaaaaaacgg gagttcggtc gcaggatcct acaaataggg
    19681 gggcggggta ttcgtcaaaa cccaatataa ctccagcgtc ttgaccgtct caacaaattt
    19741 gtcaaacagt acgggcttct gaatatagga attcacccca aggtcatagg accggacaat
    19801 atcctcatca cgtttcgacg tggtcagcac caccacagga acccggcaca ggacaggttc
    19861 ctttttgatt cgcctcaaaa catcaatgcc gtccaccttg ggtaatcgca gatccaagag
    19921 aataagtttg ggaaatcgct caggtggaag tggcggatcc ccgttaccga acaagtgttt
    19981 catggcttcc tgtccatcgc ggatcaccgt gagattattg gcgatcttgc ctgcacgcaa
    20041 cgcttccctg gtcaactcaa tgtcgtctac attgtcttca atcagcaaaa tatcaatgac
    20101 gttaatggtc atctctcctc ctttttacac cgcgagaatt ccgattcgat tgacgatgag
    20161 tccccctctt gcccttctaa agacgtgtcg gagtagccgg aggtgagttg gcaatacccg
    20221 ctgacagtga tttcccatac ccttcatgat ttttcggaag cgtgaaaaag aacgtgctcc
    20281 ctaggccctc tgccgaatcc acccaaattt tccctttgtg gaactcgaca atgcgtttga
    20341 cgatcgcaag cccgatacct gtgccttcaa tctggtcttc tgtatgcagt ctattgaaaa
    20401 ctccaaaaat tttctggtgg aactccgggg aaatgccaat tcccttgtcg cgaacagaga
    20461 agatcaattg ttcctcaagc gtctggcacc cgatgctaat gaccggaggc tccccgggtt
    20521 tggcatattt gatggcgtta gatagtagat tggcccaaag ctgggccagc cgaacccgat
    20581 ctccatagac ccatggcaag gagtcctcga tccgtaactc tgcgtgcatc tgacggatgc
    20641 ggtcttccaa atccatacga acctggagaa caatttgttc catcgacact aattcccatg
    20701 ggtgcgtgat gcgttcgatg cgggaatact ctaacagatc acggatcagt gcctccattc
    20761 gctcggcgcc tttccggatg cgctccaggt aatgccgctc ttgatgttcc agtcgctccc
    20821 cggcacgctt caagagcagg acggcaaacc cctgtatgcc ccgcaagggc gcttgcaggt
    20881 catgggaaat ggcatagaca aaactctcca gttcttcatt gctgattcgg agggactctt
    20941 catttcgctt cgcagcggtg acgtctttgc ataatccaac ggttccggcc ggttgccctt
    21001 ctgcgttccg aaggacagag agagtgaggc tgatatggac gggatacccc ttgggattcc
    21061 gaaacacgac atcacgacca atgacctccc catggacggt taattcgtcc accaatggct
    21121 tccactgacc tggatccatg aacagttccg tcacgggttt ggagaccacg gcttccgcag
    21181 catatccgag cacatgctcg gctccccgac taaacctcgt gatgttttcc tccgtgtcgg
    21241 aaaaaaagat cggatccggc gcgtgatcaa gcacatccgc caactgatcc cgtgccgccc
    21301 gatattcccg ttcggccgtg agcgcccgaa ccgcctcggt ttgcgcctga ccatattcct
    21361 gtctcaagcg gtatgtgagc caatacaaca gagccaccaa cgggaaaaag atgacacccc
    21421 ccgcgagacc caaattccaa acgatccggt ggaagggggc caggatcgca ctgcgatcca
    21481 ggttgacgag gactgtccaa ttcagacctg gaaactcgcc tattcctttg gtgcgggcat
    21541 atcctctgag gatttccact ccacgtcgtt tatgcatttc ttcgacgtaa ccagcctgcc
    21601 ccgacgcatt catgaatgcc gactgtactc caagctttct caggttgact tttccctctt
    21661 cctgtaacag ggaatcggct agaaggttgc catccttgtc caataaatgc cactccccca
    21721 tcgcggaagg gccttcaggc atctgtacag ggatcactgt ccggtcgaag acttttcgga
    21781 gatcgggcag ccccactcgt gcagtcacgg cacccaaaaa tttcgcatca gcgtcaagga
    21841 tcggagccga aaaggccacc gccaagaccc ctccagcatc ccgggaccgt tctgcatttt
    21901 gaatatgaat gccaccttta tcgcgaacat cttgaaacca ggtttcgcga ctgcggtctt
    21961 ctcctacgtt ggagcgattg gaggcggcaa taatcctgcc ttgcggatcg gtcaccgcta
    22021 tccaccggta gatagggtag gcttttttaa acaaatccag ataattgttc atggtgtgga
    22081 cgtccctacc atgaaaaact tctgatttgg caatggtttg aatatcccca taccgctcaa
    22141 acaggagcag gtccaactta tcggcaatat cgaccgcaac cctggtcaaa ctccttccgg
    22201 cagcttccag ggtttgattt tcaagctgat gaacggcgac agccccatga accaataccc
    22261 cgaccacgat gaggatacga agccatgaca accattggta ggaacttttt ggtagctgca
    22321 tcatcgcatt tcgttaaaac cccttccggg ccgtagacca gaacccacct ttcccggcct
    22381 tcgccatacc gctacacaca aagcggatac ggttccgccg cctggcatcc tcttatggct
    22441 cttcaatatt catctgttta gcctagacac aactttgagg gatcggatca ccacgaaaga
    22501 tgtggtcaat gcaggcaagt gaaataggca ctgaggaaga attcaatatc acgaccctat
    22561 ggtcgatgct aattagcatg ctcatagagc ctggtcaata ggatggattc cttacccgga
    22621 attccgtcag aaaataagga tgatgtatgg tttgcgttat tcaattcccc tgaactcaaa
    22681 accctgatac cgtccaaccc accacgcgca agcgaatttg tctcatctga ttctgtggca
    22741 agacctgaga atcaatccac aagatcaata attcccacag agggtacatt cggcttcatg
    22801 ctcagaaatc aaaaggctcg ttaaaatgaa ggaattgtgt gatggtcatt cgaagataaa
    22861 ctgacctcca agtataggaa acctttccac tatagaccgg acacgcggct cattccatac
    22921 gatgtcagcc gttattttct tggcttgtga caggcgttac ttgatatcgc ttgggaattg
    22981 gtattcggcg cacttcactt aatgaaataa ctatcctgta tgctattggg aagggggacg
    23041 atatgccccc gaacgtcctg ccacaacaaa acgtcacgac tcaagaaacc aaccggatcc
    23101 caaaaacacg accgaacgag tgtccgtgta agaggggcat tcagttatcc cctcctccca
    23161 cctcacccaa gggaacgtat ccatttatca attcttttat gccatctacc aggctagttg
    23221 acgcgtcaat accaggagag cgccggggaa atgcaggaga caaacattac gtatgaggaa
    23281 ctggaaaacc aataaggaaa tgtgtcgtag actgataagg cgatgggtcc agccgggata
    23341 acttttataa gttcaaaatt tgatacgacc ggttccgtag gggtattgca ggtgagaacg
    23401 ccggggcaaa cttgtggggg tttttacctc agtcctctgg ttctagacag catcagagat
    23461 ggaaaatcct tttcatattc gctcgacata gcaaaaaact ggtcaaaatg aacatgatat
    23521 cgatcacatc tcttcgccat catcccccat ctttcattcg taaactgccc ttgtacgaaa
    23581 cgggaggagg ttggtacatc acgaagttgt tcccccgagc gttctaatat acgccaggac
    23641 ttctttcatc tcctcctctg ataaggcata ttcccacttc cccatggccg tgttgggatg
    23701 cccgttccgg atcgtctcca ataacgcctc atcggttttg ttctgggttg ccgggctttt
    23761 caaatcggca ggcggcggat caaagaaagg ataggcctct tcctttccgg cttttccgtg
    23821 gcaggccgaa cacaatcgtt gatagaggcc tttacccttt tccactgagg tatcctgtcc
    23881 gtgtaccacc tggaccttta cacccggcat caaccagagc gtcagcccga tcagtgcgat
    23941 gctcccccat tgccgccaaa caaaatttgg atgttcagta accatgttca gcctccctcc
    24001 gtccccccgg aagaataatg actcagcagt ccgacggttg cactgtatgc agacagccct
    24061 gcaaccagtg ctgtagctca accaacaact tcccaacgac tttttgggca gcgtgcacgc
    24121 caccataagc atcttcgctg gatgacgctt catgaaattc aaaactccgc gtcccgatga
    24181 cacgaggcgc aaacaccgtc gtcagttgtg cccgcagggc caatcgaatt cgactcggct
    24241 gttgcgtgaa ttcctgaacc aacgccacct gggagaggtc caagcgatag tcccctcgaa
    24301 gcacggaagg gagttgaatc accgcacccc attccccact gctttcgagt gcgttcgtga
    24361 ttatcggggc cagcattcgg gcgggggaat ccacccattg gctggtggca aaatagcgaa
    24421 gttcataggg aacctgttga taggccattc gttgcgattc atagcccggc gccggctgag
    24481 gaagggagac cagcagactc ggcaagttcg atggacggaa ttcgtcagat gcccgggttt
    24541 ctcctccctt ctctatttcc aggacatacg accgcatcga ctcatccgtc cctcgagaga
    24601 gtgcacaagc ggaaagtgcc gtcgaaaggc atatggccgc cagcaggaac atggccttca
    24661 tgtctttact caccgggacc tctgggttga gatggtcgac cataaatcag cgagtcgggt
    24721 tcctgctcga tttgatgagc cactcgttgc aacgtgtttg tcagctgccg gagttcggtc
    24781 actaattgcc cggtttcacc caatgtctcg cgtgtgaatt gttggatgtc cggacggctt
    24841 tcctgcacga cggcctccaa cgtctttccg gttcgggcca attcttccgc caccgtcttg
    24901 accgctgtga cactctggtg aattcgcgcg accagtcttg gcatgtcttg gttcaccgtt
    24961 gaggtcagga tggccagatt ctcggcggct tcaaaggccc gatcaattcc ctgggctatc
    25021 gtttcatgct gtctgcccaa tgtgtgggac accgtggcaa ggtctttcac aatcccttcc
    25081 atctttaagc ggttctcctc attcatgaaa gtcgcagcat tttcacttaa cccatgcagg
    25141 ctattcaaaa gcctggaaac cccttgctct gaaaggagac gcgaaatggc catatccaag
    25201 cgaaagaaga gcgagggttt cgtctgaatg accggatatt cctgaccggg ctcgggttcc
    25261 aatctcggag cctcgcggct ccctccttcc aaattcatcg tcgccaatcc ggttaatcct
    25321 tgagtttcca acgtggcgac cgtatcggtc ttgatgggtg tgccctgaag aatatcaagc
    25381 gtcaaacgca cctcctcggg attatccgga ttcaacgcaa tctctttgac tcgccccaca
    25441 ttcactcccc ggtattttac cgtcgagttt acgcttaacc cggacacgga ttgacgcatg
    25501 taggcatggt actggtcgta cacaccgcgg taatctgatt ttcccaacca caacacgatc
    25561 cccacggcaa cgacacccag cacaaagacg aacactccga caatgaggta attccctcgc
    25621 ggctccatca tgaagcctca tattgactct gggccgcccg tccccggggc ccatggaaat
    25681 attcatgaat gatcggatcg cgtgattccg ccagttcctg catcgttcct acacgcagga
    25741 ttgtgccgtt cccgagcacg gcgacacgat cggcaatgcg ccacagagaa tcaaggtcat
    25801 gcgtgaccat aaccaccgtt aatcctaata ccgctttcaa ctgagggacc aactcatcaa
    25861 atcctgc
//
LOCUS       DATIAL010000261         4058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_800823, whole genome shotgun sequence.
ACCESSION   DATIAL010000261 DATIAL010000000
VERSION     DATIAL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800823"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VLA60_08105"
     CDS             complement(<1..928)
                     /locus_tag="VLA60_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09362.1"
                     /translation="MKRLIVHGQREPLDLKSAIPDFPASLNDLVTLKHSARVQRARAG
                     QPPLEIDDLRPDDLIQLELDQGLKFWVRVDDLETDFGLTPSRGPAGDVVELPHVLPLG
                     TPSRGIVGNWVVKAFKVFGLDPVGATTDLIKDKVEGVLKPGPGLYRWEGKESSDYTRI
                     KSLEKERTDNPWLVFIHGTASSTDGSFGGLWEEGANARMLQLLAHYSKRVLAFQHRTL
                     SQNPIQNALELAQSFPKGARLHVVSHSRGGLVGELLCRSIMEGRFPFDQDDLQVFDHL
                     DRKDDLKQLEQLGDLLHQKQLVIERFVRVGCPA"
     gene            complement(1640..3115)
                     /locus_tag="VLA60_08110"
     CDS             complement(1640..3115)
                     /locus_tag="VLA60_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSF09363.1"
                     /translation="MIVKRPVFLMFLILCLPGCLVGPDYQQPVLNVPEDWNSMTLEGA
                     PSQLPITIGQVPEAGWWRSFGNDELNDLIEQALDRNHDLRKAAFRVQEARAIAYGSGA
                     GLYPNLSLKGDYSRIRRSETILVAPTGGTPEGFAPPGANFDIWRGALDLRWELDLWGR
                     IRRGQEAFSAEALGSEMNRRGLLLSLVGEVGQSYFRLRELDEQIEIAERNLALQQDSL
                     SIIRSRAQAGLVSDLDVKREETLVAQTASEIPEFRRQRAVQLHQLEVLTGANPGTLLL
                     SAKPLRSVVVHPTIPVGLPSDLLQRRPDILEIEETLKAANARIGEARAYFFPSLSITG
                     TGGFLTSEFDQWFKWGSRNMTIGPAVTLPIFEGYTNLARLEVAENRYQQMLEDYHQTI
                     LNAFREVADVLVALQTRKEQLVNQRQQVAAARESLELADIRYRKGLVTYVDVIDAQRI
                     LLTAELGVVQTERARLTAMVDLFKAVGGGWEQENLAQVKPQ"
     gene            complement(3126..>4058)
                     /locus_tag="VLA60_08115"
     CDS             complement(3126..>4058)
                     /locus_tag="VLA60_08115"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF09364.1"
                     /translation="PPPPMEAPPQNPYPVTVAAAGIIEAVNENVRIAPPVSGLVTKVY
                     VTVADHVEQGAPLFQLDDRELRAQLQTKTDSLPSAAARIAEQEIRLRDVQDQLKRLQS
                     VTDRRAVSEDDVKRKWHEAEGAKRALIRARADFHLAKTEREEVQALLNRLTVRAPRAA
                     TILQVNVRAGEYATVNAIEPLIILGDTETLQVRADIDEVNAPLVVPGSPAVAYLKGYT
                     DQAIPLTFDRIEPYIVPKRSLTGDNRERVDTRVLQVIYRFERPSFPVYVGQQVDVYIE
                     RSTKGVSGQDQNRSENDAQQSDSFRLLSKRGEAQ"
BASE COUNT          973 a         1176 c         1015 g          894 t
ORIGIN      
        1 gggctggaca tcccacccgc acaaaccgtt cgatgaccaa ttgtttttgg tggagcaagt
       61 caccgagttg ctccaattgc ttgagatcgt ctttccgatc gagatggtca aagacctgaa
      121 gatcatcctg atcaaagggg aatcggccct ccatgatgct acgacacagt aattccccga
      181 cgagtccccc acgggaatgg gagaccacat gcaagcgggc cccttttgga aatgactgag
      241 ccaattccaa ggcgttttgg atgggattct gagaaagcgt gcgatgttga aaggcgagca
      301 cgcgtttgga gtaatgcgcg agcagttgca acatgcgcgc attcgcgcct tcttcccaca
      361 acccaccgaa actgccatcg gtcgaagagg cggttccgtg aatgaagacc agccacgggt
      421 tatccgtcct ttccttttcc agggatttaa tccgggtgta atcggacgac tcttttcctt
      481 cccaccgata gaggccggga cccggcttca atacaccctc gaccttgtcc ttaatcaagt
      541 ccgtggtcgc gcccacggga tccaggccaa acactttgaa cgctttaacc acccaattgc
      601 cgacaatgcc ccgacttgga gtcccgagag gcaacacatg aggaagttcg accacgtcac
      661 cggcaggccc acggctggga gtcaatccaa aatccgtttc cagatcatcc acccggaccc
      721 aaaatttcaa tccctgatcc agctccaact gaatgagatc gtccgggcgc aaatcatcaa
      781 tttccagagg gggttggccc gcgcgagccc gttggaccct ggcactgtgt ttcagggtga
      841 ccaggtcatt cagactggca ggaaaatcgg gaattgcaga tttcaggtcg agcggctcac
      901 gctgtccatg aacgataaga cgtttcattg tttattcctc acaaaatgtt gttggcatga
      961 ccatccatgt ttcccgtcaa aacaagcagt tcataattgt tatcgacgat gacccaaatg
     1021 cgttccattc gtttgcccat tcaaaaaatc ggaatcccat tacagcaaat ccgtcgaaaa
     1081 attcctaccc tttgctccgt caccacagtg aagcgcggac ggaccgttga gaaatcatcg
     1141 acatgaaagg tcttgaagga aacattgagg tgggagaggt tagaaaggaa gggatttcgg
     1201 cgaatgtccg tcgaggccct tacccaagcc aaccatcatg caaacaaaat taagaaagaa
     1261 acgggcttat gatccgacaa taaaaagggg ggaacataca acagaaattc cgactcacca
     1321 ctggccagaa tgactaccag tattgtagag aaccctgggt gggcgatgcc cgcacttctt
     1381 tttcccgact tccctcgacc tttctaaaca aagcacaccc catccccagc cgccagggtg
     1441 tcatcctgag gaaaacgaaa gatctgtgcc caaaccccct aacttccgct aggcgagata
     1501 catcgccttc agctcagcat gacaatagga aaagagtaat aaggaaagag aaccaaggga
     1561 tagggtatcg aaaatgggga tgacgggttg aactaacctt ttcagaaata cttgacggga
     1621 gatgctcaca ctggatccac tattgtggct tcacttgagc caggttttcc tgctcccaac
     1681 cgccgcctac ggctttgaac agatccacca tagccgtgag acgtgcgcgt tcggtttgga
     1741 ccacgcccaa ttccgcagtc aagaggatgc gctgggcatc tatcacatca acataggtga
     1801 ccaatccctt gcgataacgg atgtctgcga gctccagcga ttcccgagcg gcggctactt
     1861 gttggcgttg attcaccagt tgttcttttc gcgtttgcag ggcgaccagc acatcggcca
     1921 cttccctgaa cgcattcaga atggtttgat ggtagtcctc caacatctgc tgataccggt
     1981 tttcggccac ttctaatctg gccagattgg tataaccttc gaagatgggc agggtgaccg
     2041 ccgggccaat cgtcatgttg cggctgcccc atttgaacca ttgatcgaat tcgcttgtga
     2101 gaaaacctcc cgttcccgtg atagacagac tggggaaaaa gtacgctcgc gcttccccaa
     2161 tgcgcgcatt ggccgctttc agggtttcct cgatttccag gatatcggga cgccgttgca
     2221 gcagatcgga aggcagaccc acgggaatcg tcgggtgaac caccacggac cgtaagggct
     2281 tggccgacaa cagcagtgta cccggatttg ctcccgtcaa cacctcgagt tggtgcagtt
     2341 gcacggcccg ttgacgacga aactcgggaa tttccgaggc cgtttgagcc acaagggttt
     2401 cctctcgttt gacatccagg tcggatacca gacctgcctg agcgcggctc cggataatcg
     2461 acaatgaatc ttgttgaagc gctaaattcc gttcggcaat ttctatttgt tcgtcgagtt
     2521 cacgaagccg gaaataggat tgcccaacct caccgaccag gcttaacaac aaaccacggc
     2581 gattcatctc actccccaac gcttccgccg aaaaggcctc ttgcccccgt cgaatccgac
     2641 cccataaatc cagttcccat cgaagatcga gggccccgcg ccagatatca aaattggcgc
     2701 ccggtggcgc gaacccttcc ggcgttccgc ctgtcggagc cacgagaatc gtttccgatc
     2761 gacggattcg ggaatagtcg cccttcaagg aaagattggg atacagaccg gctccggatc
     2821 catacgcaat cgcgcgcgcc tcctgtaccc gaaacgcggc ttttcgcaga tcgtggttgc
     2881 gatccaaagc ctgttcaatc aagtcgttca attcatcgtt gccaaatgat cgccaccacc
     2941 cggcctccgg cacttgccct atggtgatgg gaagctggga aggcgcgcct tccaaggtca
     3001 tgctgttcca gtcttccggc acattgagaa ccggctgctg atagtccggc cccaccaggc
     3061 atcccggcag acaaaggatc aggaacatca ggaatacggg tctctttacg atcatgcgtt
     3121 ttccctcact gagcctcacc tctcttcgac aagaggcgaa aggaatcaga ttgctgcgca
     3181 tcgttctccg atcggttctg gtcctgtcct gaaacccctt tagtagagcg ctcgatatag
     3241 acatcaactt gctggccaac ataaaccgga aaagaagggc gttcaaaccg ataaatgacc
     3301 tgaagcaccc tcgtatcgac ccgctcacga ttgtctccag taagggatcg tttgggcaca
     3361 atgtagggtt caatgcgatc gaaggtgaga ggaatagcct gatccgtata ccctttgaga
     3421 taggccaccg cgggacttcc cggcaccacc agcggcgcgt tgacctcatc gatatcggcc
     3481 cgtacctgta aggtttccgt atcccctaat atgatgagtg gctcgatggc attcaccgtg
     3541 gcatattccc cggctcgaac gttgacctga agaatggtcg ccgcacgcgg tgcccgaacc
     3601 gtaagacggt ttaataaagc ttgcacttcc tcccgttctg tttttgccaa atggaaatca
     3661 gcccttgccc gaatcaacgc ccgcttggca ccttccgctt catgccattt tctcttcaca
     3721 tcatcttcac tgaccgcccg acggtcggta accgactgaa gccgctttaa ttggtcctgg
     3781 acatcccgga gcctgatctc ctgctcggcg atccgcgccg ctgcggatgg gagcgaatcg
     3841 gtcttggttt gcaattgggc ccgcaactcc cgatcatcca actgaaacaa gggagctccc
     3901 tgctcaacat gatccgccac cgtgacatac accttcgtca ccagaccgga gaccggaggc
     3961 gcgatcctga cgttttcgtt caccgcctca ataatgcctg ctgcggccac cgtgactgga
     4021 tacggattct gaggaggagc ttccatcggt ggaggcgg
//
LOCUS       DATIAL010000262         2163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_48474, whole genome shotgun sequence.
ACCESSION   DATIAL010000262 DATIAL010000000
VERSION     DATIAL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48474"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VLA60_08120"
     CDS             complement(<1..667)
                     /locus_tag="VLA60_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSF09365.1"
                     /translation="MSRKYLAFDIETAKILPENFGDLHDHRPLGITCMATWCSDESSA
                     VTFYSKNPDGSPAPQMTKADVTAFVEHLKTKLQEGYTIITHNGLGFDFVIVAEESGQV
                     DDCRELAMSHVDMMYHFFCGKGFPIKLNAAAKAIGVSKPANVDGSVAPKLWKDGDFQT
                     VLDYVAQDCRLTLDVAEASEQAKKISWITGRGTTSNFELPGGWLTVQEASKLPLPDTS
                     WMDQ"
     gene            complement(780..1151)
                     /locus_tag="VLA60_08125"
     CDS             complement(780..1151)
                     /locus_tag="VLA60_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09366.1"
                     /translation="MSFTQFRDVKEQSVEPDDRNVRVRRLSFIFLAFLGVFAMGCGLF
                     PQEQLYGWKGVHREELIRVMGTPTKQTALPDGGQRLEFIQRITRHPLGGEVYGTSYEC
                     HKTFELDAEGIVQKAVSETPC"
     gene            complement(1238..1768)
                     /locus_tag="VLA60_08130"
     CDS             complement(1238..1768)
                     /locus_tag="VLA60_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09367.1"
                     /translation="MAPLPASLSADQTIVFGKVQASLTGPTTRWYTPQVRFFELMNHS
                     TKERFRVDIESADSPFFLAIPPGEYELVRLQINEGAFRSMAQLNSTFQVNQGGLNYLG
                     KWEVKIHPPTYLRLVDVSVDTDLEDAKAQVLELHPDLAGLPINSQLPTPKDSETRLVE
                     ISPYPRVWWFCRHLTC"
BASE COUNT          570 a          566 c          478 g          549 t
ORIGIN      
        1 gctgatccat ccatgacgtg tcaggtagag gtaacttgga ggcttcttga acggtcagcc
       61 atccaccagg caattcaaaa ttagatgttg tgccacgtcc ggttatccaa ctaatttttt
      121 tcgcttgttc actggcttca gccacatcca acgttaaccg acaatcttga gccacataat
      181 ccaagaccgt ctggaagtcg ccatctttcc acagtttcgg ggcgacggag ccatccacat
      241 tcgcgggctt actcacacca atggccttcg ccgcggcgtt gagcttgata gggaaccctt
      301 tcccgcaaaa gaagtgatac atcatgtcca cgtggctcat cgccaattca cggcaatcat
      361 caacctggcc ggattcttcc gccacaatca cgaaatcgaa tcccagccca ttgtgggtga
      421 tgatggtata accctcctgc aatttggttt tgagatgctc gacaaaggcg gtgacatccg
      481 ccttggtcat ttgcggcgca ggagatccat ctggattttt cgaataaaac gtcacagctg
      541 aggattcatc gctgcaccag gtcgccatac aggtgatgcc gagcggccga tgatcatgta
      601 agtcaccgaa attttccggc aaaatttttg ccgtttcgat atcaaaggcc agatatttac
      661 gggacattgg tcaactctcc ccacgattga gccggatggc ctgattaaag gtcatgtcgc
      721 acgccatttc acggttgttt tggggttttc tggaacaatg cggtcttagt gctatttgat
      781 caacacggcg tttcagaaac ggctttttga acgatacctt cggcatcaag ttcaaacgtt
      841 ttatgacact catacgaagt gccatacact tcacctccca atggatggcg agttatccgt
      901 tgaataaatt ccaaacgttg cccaccgtca ggcaaagccg tttgcttagt cggcgtcccc
      961 atcacccgaa ttagctcttc acgatgcacg cccttccaac catacaattg ttcctgaggg
     1021 aaaagaccgc agcccattgc aaaaacccct aaaaatgcta aaaaaataaa cgacaatctt
     1081 ctcactcgta cattccgatc atccggttcg acagattgtt ctttaacatc cctaaactgg
     1141 gtaaaactca ttggcggctt ttgacgtatt cgctttctga atcggcattg tccatctgca
     1201 atccatcgtt aggacctgat gtttctcata tctaaaatta acaggttaaa tggcggcaaa
     1261 accaccatac ccgaggataa ggagatattt ccactaaacg cgtttccgaa tccttaggtg
     1321 tcgggagttg cgaattgatg ggaagacccg ctaaatcggg atgaagttca aggacctggg
     1381 cttttgcgtc ctctaaatca gtgtccacgg aaacatcaac caaccgaaga tacgtcgggg
     1441 gatgtatttt cacttcccat ttccccagat aattcagccc accctgattc acctggaacg
     1501 tcgagttcaa ttgggccata gatcggaatg ccccttcatt gatctggagc cgcaccaact
     1561 catattcgcc tggcggaatt gcgagaaaaa agggcgaatc cgcagactcg atatccaccc
     1621 ggaaccgttc cttcgtagag tgattcatga gctcaaaaaa tcgaacctga ggagtatacc
     1681 atcgggtcgt agggccggtg agacttgctt gaaccttacc aaacacgatt gtttgatcgg
     1741 ctgaaagact ggcaggcaac ggcgccattt tcgtggcaca tcctaggaaa aggacaacta
     1801 gtccgaggcc taacatcagg attctgattt ccttcatgac cacatagtct ctcgtgtttt
     1861 tcatcaatcg gtctgaaaag aaagttttgg cctccaaaca gaactacttt ctttttgtgt
     1921 gaaccaaatc gcatgcacca cataccgaaa aagtggtctc ccggatttat ctaggcgctc
     1981 gaagtaagta attactataa aacagccagc gggcaccgac ccaggagacc cacttccact
     2041 atctcattat atcagaggga tgacgtgctc ctgacatgac gctgacacta accgagcgcg
     2101 atttttgttc atgatgaggg atgaccacag attcaatagc tagccagtag cctctgcgcg
     2161 tgg
//
LOCUS       DATIAL010000263         3651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1123487, whole genome shotgun sequence.
ACCESSION   DATIAL010000263 DATIAL010000000
VERSION     DATIAL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123487"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /gene="aroB"
                     /locus_tag="VLA60_08135"
     CDS             complement(<1..848)
                     /gene="aroB"
                     /locus_tag="VLA60_08135"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HSF09368.1"
                     /translation="MKTSEFSKHVPVSLGDRSYQVRIQPHILPQIGRVLQDVGLSGRV
                     AIVTNPVVNELYGRVVFRALKRCGFSPFLVVIPDGEKAKSMHWLSKILDELVTQRLER
                     QEVVLALGGGVVGDVAGFAASVYLRGVSFVQVPTTLVAQVDSSVGGKTGVNHPIGKNL
                     IGAFYQPRVVVIDPQTLHSLPKREWVAGLAEVIKYGMIADPKFFEYLEQHVDDLREQA
                     DDVIPTVIRRCCEIKAEVVGGDERESGRRRILNYGHTVGHALETWGRYRTWIHGEAVG
                     IGMVQEA"
     gene            complement(928..2088)
                     /gene="aroC"
                     /locus_tag="VLA60_08140"
     CDS             complement(928..2088)
                     /gene="aroC"
                     /locus_tag="VLA60_08140"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HSF09369.1"
                     /translation="MLRYLNAGESHGRGLMAVVEGVPSGLPVTAEGINVDLIRRQGGY
                     GRGGRMRIEKDRIEFICGVRKGKTLGNPLGLLIWNKDWENWKDIMASEPGPPSTERVV
                     TRPRPGHADLVGAIKYGHSDIRNVLEKASARETAIRVAIGGVAKSLLAEFDMRVVSYT
                     MDIGGIAAPSPNDPLIAYEHAEQSEVRCHDPETAKKMVEQIRAAKHKGDSLGGIFEVV
                     VTNVPIGLGTYAQWDRRLSARLAFAAMSIQAMKGVEIGMGFASARRFGSEVHDDIYFD
                     KATGQFTRKSNNAGGLEGGITNGQPIVLRVAMKPIATLYTPKDSVDIETKEPFEATVE
                     RSDICTVPAAGVVGEAVIAYEMANALIEKFGGDTLDEMKRNFEAYQEYVRTF"
     gene            complement(2325..2540)
                     /locus_tag="VLA60_08145"
     CDS             complement(2325..2540)
                     /locus_tag="VLA60_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09370.1"
                     /translation="MIHGRDGRHAIIQVSPSKRVLVQTGSELARSGWTIKTISEEEVL
                     LEYLSSAASLKGPSSPRTFILSFPTIQ"
     gene            complement(2911..3444)
                     /gene="pilO"
                     /locus_tag="VLA60_08150"
     CDS             complement(2911..3444)
                     /gene="pilO"
                     /locus_tag="VLA60_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HSF09371.1"
                     /translation="MLVSLLGIHFLMWDTMNDSIKQLQEEVSRLDQESRGLTQKTESL
                     KTIEKEVNSLRDNLATRIQQFPENIESKIFRRDVIEIAKRRHVTVRVWKPEPPLTELQ
                     LSEFSIPIALRVEGNFQGTVQFLDDLRQLAWVERIPSITLAGKPDNEHSPLVSTNLVM
                     HGLTPLGIEHVQQLLKT"
BASE COUNT          946 a         1024 c          747 g          934 t
ORIGIN      
        1 gcttcctgca ccatgccaat tcccaccgct tctccatgaa tccatgttct atagcgaccc
       61 caggtttcca gtgcatggcc tacggtatgg ccataattca aaatgcgtct gcgtcctgat
      121 tcccgttcat ctccacccac gacttcagct ttaatttcac aacaccgccg aatcacggtc
      181 gggatgacat catctgcttg ttctcgtaag tcatcaacgt gttgttctaa atattcaaaa
      241 aacttcggat ccgcaatcat gccgtactta atcacctccg ctaaccctgc tacccattca
      301 cgttttggaa gagaatgcaa tgtctgcgga tcaataacca cgacacgtgg ctgataaaac
      361 gccccgatca aatttttccc tattggatgg ttgacccctg tttttccacc cacgcttgaa
      421 tctacttgag ccaccaatgt cgtcggcact tggacaaatg aaacaccgcg cagataaaca
      481 gaggccgcaa accccgccac atctcccacg acccctccac ctaacgctaa caccacttcc
      541 tggcgttcaa gtcgttgggt aaccagttca tccagtattt ttgacagcca atgcatggat
      601 ttggcctttt ctccatcggg tatcaccaca agaaatggag aaaatccaca acgttttaag
      661 gcacggaaaa ctacccgccc atagagttcg ttgacgactg gattggtcac aatggcaact
      721 cgccctgaca atccaacatc ctgaagcaca cggcctattt gaggcagaat atgaggttga
      781 atacggactt gataactccg atctcccaat gaaaccggaa catgtttgga gaattcagag
      841 gtcttcatct agcggaagtc atcccaaagc agttttattc caagaaacga tagaaacctg
      901 ccacgagagg atttcaaggc aaaaggatta aaacgtgcgg acatactctt gataagcctc
      961 aaagtttctt ttcatttcat ccaacgtatc tccaccaaat ttttcaatca gggcattggc
     1021 catttcatag gcaatcaccg cttcgcccac gacaccggca gccggaacag tgcaaatatc
     1081 tgaacgctct accgtggcct caaacggttc ttttgtttcg atgtccacgc tgtctttggg
     1141 agtatagaga gtcgcgatgg gtttcatggc cactcgcaga acgatcggtt gcccattcgt
     1201 aatgccgcct tccaacccac cggcattgtt cgattttcta gtaaattgac ctgtggcttt
     1261 gtcgaaataa atgtcgtcat gcacttccga tccaaaacgg cgggcagacg caaatcccat
     1321 accaatttct acccccttca tcgcttgaat actcatcgca gcaaacgcca agcgagcact
     1381 taggcgccga tcccattgag cgtaggttcc caacccgatc gggacgttcg tgaccacaac
     1441 ctcaaaaata ccgcctaagg aatcaccttt atgcttggct gcccgaatct gttcaaccat
     1501 tttctttgcc gtctcagggt catggcatcg cacctcagat tgttctgcat gttcatacgc
     1561 tatcaacgga tcattcggac tgggagctgc gatccctcca atgtccatcg tataactgac
     1621 cactcgcatg tcaaactcgg ccagcaacga tttcgccaca ccaccgatgg caaccctgat
     1681 cgctgtttcc ctggcactgg ccttttctag cacattgcgg atatcgctat gaccatattt
     1741 gatggctcca accaaatctg catggcccgg ccgtggacga gtcaccactc gttcagtgga
     1801 agggggacca ggttcggacg ccatgatgtc tttccagttt tcccaatctt tattccagat
     1861 caagagtcct agcggattcc ccaaagtttt cccttttcga actccacaaa taaattcaat
     1921 ccggtccttt tcaattcgca tccgaccgcc acgcccatat ccgccctgtc ggcgaatcaa
     1981 atccacatta atcccttcgg ccgtgaccgg caatccagac ggaacccctt ctaccacagc
     2041 cattagacct cttccatgag attcccccgc atttaaatat cgcaacatgg cattcctttt
     2101 tggctcatta aatactcatg ccggcagcat gttccctctt aagatgttcc cacccttact
     2161 gaccagaacg gtctacccaa gcctgcacct acagccttgg gatacggata atgatcaatc
     2221 accagctccc attccggaac ccgtcaaaga gtcccacgcc ggactcttag agtacagatt
     2281 atgaaggttt tttcaggctt tctcaaccag aatattcaca atgattattg gatggtggga
     2341 aaggaaagaa tgaaggttct tggcgaggaa ggccccttca atgaggcggc tgaggagagg
     2401 tactcaagaa gcacctcctc ctcactgatg gtcttaatcg tccatcctga tcgagcgagt
     2461 tcagatcccg tttggaccag aactcgcttc gatggagata cttgaatgat cgcatggcgc
     2521 ccatctcggc catgaatcac gccaagaagt ctccaatccg gattatccag caagggaaca
     2581 attttcgatt ccttatctga tggcgccgta actttagggg ttgatagacg cgggggaggt
     2641 atccgcttga aagggtctcg acgattcccg tcatttcgtc gggaattgtc cttaacgaaa
     2701 ctgggtctaa gtagcctgtt ttggtaagag atcgatgtgg aggcgccttc cgcagccacc
     2761 actcccatta ccccccccca attaagcaca atccaaacac agaccgagta gcacacgacc
     2821 cctttcaggt aggcagacat tgtggagacc ctgctttgaa tgtttatgac tccaggatct
     2881 cgcaatcccg cctgacattg atcggttcga ttatgtttta agcagctgct gaacatgttc
     2941 aatccctaag ggagttaatc cgtgcatcac cagatttgtg ctgacgagag gtgaatgttc
     3001 gttgtctggc tttccggcca gggtaatgga agggatacgt tcaacccatg caagctgccg
     3061 caagtcatcc aaaaattgaa cggtcccctg aaaattccct tctactctga gagcaatggg
     3121 tatcgaaaat tcagaaagct gaagctcggt caaaggaggt tcaggtttcc acacccgaac
     3181 ggtcacatgc cttcgcttcg caatttcgat gacatccctt cgaaagattt ttgattctat
     3241 attttcagga aattgttgaa ttcgagtggc taaattgtca cgcaagctgt tgacttcctt
     3301 ctctatggtc ttcagtgatt ctgttttttg ggtgagccct cgactctcct gatctaggcg
     3361 agaaacttcc tcttgcaact gcttaatcga gtcattcatc gtatcccaca tcaggaagtg
     3421 aatacccaaa agagagacga gcattattcc acccagaaaa ctgaatcgac accaaagaga
     3481 taatgacgtc cactgtgcca tcatgtagtc acctgttgct cccatacaaa acgaatcaaa
     3541 aatgaaaagg cggctgattc tcctcctgag tgatcaagaa tttccactac aggtaaactc
     3601 acaataattc cgtcattctc caacccctcc ataaatttac caatatcttc t
//
LOCUS       DATIAL010000264         5424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_156567, whole genome shotgun sequence.
ACCESSION   DATIAL010000264 DATIAL010000000
VERSION     DATIAL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156567"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VLA60_08155"
     CDS             <1..336
                     /locus_tag="VLA60_08155"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF09372.1"
                     /translation="IHVNHFSSSLSCGWHHIRPLFIMALLLAAFGCASPLGHDAEVSE
                     RNEYIAARIKVALVQEGLDAAPIDIAIRNGVVTLGGFVEKEPQRQAAEKAAINVPGVD
                     SVLNNIQVK"
     gene            complement(816..1685)
                     /locus_tag="VLA60_08160"
     CDS             complement(816..1685)
                     /locus_tag="VLA60_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09373.1"
                     /translation="MSLFKKFAAGWKTTVLLGSCVYLLVGIAAASGGEKSSPDPSKPH
                     STMEQGKKDGDQHQQFTLLPGHRVINGVVESVKADQAKVNSGDTGEISPRYLSMERAK
                     EKGFTLKQGDNVTIVVNDKNHVVDYHLTGSKGQSHHQVVKGKLAQPLKVGQEQAIIKT
                     KEGKEQSFAVRPLARSEVAAIPFDTEGLFLIDETNKIASATLTQDVTSEDDWARSTAY
                     NVYRHIEGVIQEKPEKKSVTIQTKNKESLTLPVWDYLQKELTELSKGMPVTLLVDKND
                     KVADIAYIPRSEK"
     gene            complement(1790..2341)
                     /locus_tag="VLA60_08165"
     CDS             complement(1790..2341)
                     /locus_tag="VLA60_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09374.1"
                     /translation="MRPFASKFSIQTFGSVLLATAMMLLPGTVLAGQGEVSSPVGSGS
                     TSVPDVQTNMQSGADIQWSDQLQRERDQAKQSRDQARHERDQAQQERDQALQANKSPD
                     DSSDSMKSIQGQTSSAKMGSGADKQENARLIQDRDQFLKERDALQKETSDLRQERDRA
                     MSSISGGSALDAVDRTMPVTPRH"
     gene            complement(2669..4435)
                     /locus_tag="VLA60_08170"
     CDS             complement(2669..4435)
                     /locus_tag="VLA60_08170"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HSF09375.1"
                     /translation="MRYLALATDYDGTLALHGRVHESTVRSLEKVLTTNRKLLLVTGR
                     ELDDLLNVFPRPHLFDWIVAENGLVLYKPSSKETITLADMPSEDFITGLRSRGIPISV
                     GRNIVSTVRPHETVVLEVVRDLGLDIQLIFNKEAVMMLPAGFSKATGLEKALRKMGLS
                     PHNVAGIGDAENDLSFLGMVECSAAVGNALPAIKERADIVTEGEQGDGVVEFIRHLLT
                     DDLQSIDPSLHRHYVVLGSTETEQEVRISPYGPNLLLAGSSGSGKSTLSTGIIERLTD
                     KHYQCCIIDPEGDYESLEQAVVLGDMNHTPNTKEVIDILENPDTNLVINLVGLAIQER
                     PSFFKNIFKDLQDFRDRTGRPHWIVLDETHHLMPAEWDIPFSLFTPSPHSLLMITVHP
                     DQMAAVALAAIDCVVVIGKSPGQRLEAFTNSVGESLPSFEDHPVQPGEAIFWKRSGGA
                     LPIRFRIHPNRSERRRHRRKYAEGELGPDRSFYFQGPEGKLNLRAQNLMLFLQLAEGL
                     DPETWLYHLKRHDYSSWAQDCIKDKALAAEIRKIESTESLEHGQSLAHIKAAIESRYV
                     LSPHSPSPSPPHTPSTPSSPST"
     gene            complement(4797..>5424)
                     /locus_tag="VLA60_08175"
     CDS             complement(4797..>5424)
                     /locus_tag="VLA60_08175"
                     /inference="COORDINATES: protein motif:HMM:NF036542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HSF09376.1"
                     /translation="RTRMVSHLLNIHQDLAKQVAKGLRLKDMPKPADAAKPTREDLEQ
                     SPALSILLNSPNTFKGRKLGVLITDGVDIKLFKSLKSAVKSEGAIMEIVAPGVGGVEA
                     SDGTWIEAEQKIDGGPSVLYDAVALLPSEEGGLALAKVPAARDFVADAFAHMKFIGYV
                     EAVTPLFQKAGVQESRDAGFIALDKGKGSTQFLEACRKLRFWDRAAAS"
BASE COUNT         1307 a         1370 c         1316 g         1431 t
ORIGIN      
        1 atacatgtga accatttctc ctcttcctta tcatgtggtt ggcaccatat ccgccctttg
       61 ttcatcatgg cccttttact cgcggcgttc ggatgtgcct ctccgcttgg ccatgatgca
      121 gaagtgtcgg aaaggaacga atatatcgcc gccagaataa aggtcgcgct tgtacaggaa
      181 ggcctggatg ccgctcctat tgacatcgca atcagaaatg gggtggtaac cttgggaggg
      241 ttcgttgaaa aagaaccaca acggcaggcg gcggaaaagg ccgcaatcaa tgtacccgga
      301 gtggattccg ttctcaataa tattcaagtc aaatgacata tctcatctca agacatcttc
      361 cgctttgaca attgctcatt ccctcatagg ttaaattgaa aattatagac tgaacttgcg
      421 cacttaaccg gtttcgcacg caggatggca gcatcatact ctcgagaggg agcttgacgg
      481 tcgcgatgtg gacgaggaag ggcaatgtca acgagagtga atatcggaat tcccagatgg
      541 gagcgtggtc tccgatgatg tggaaaagtt cgtgggatta tccggtttcc tgaaacatcc
      601 tcgtccagaa cgtgtcattc aaattgacct caaagttgtg atcaactctg agacgccccc
      661 ctcctacgaa ggtgccgggc tctgtggcgc ccgttcgatc tggagtaagc aggtcccgtc
      721 gcgatgattt caatcagaga acatggctct ggtatgcgaa ctcccgtttg cccacttcca
      781 ggtcgaggct cacacccgct ttccaatcta ggaacctatt tctcactcct aggaatatag
      841 gcaatatcag cgactttatc attcttatct accaaaaggg tcaccggcat gcctttcgaa
      901 agttccgtca actctttctg taaataatcc cagaccggca atgtcaggga ttccttattt
      961 ttggtttgaa tcgtcacgga tttcttttcc ggtttttcct ggatgactcc ctcaatatga
     1021 cgatagacat tatatgcagt ggaacgagcc caatcgtcct cggacgtgac atcctgggtc
     1081 aatgtggcac tcgcaatctt attggtttca tcgatgagaa acagcccctc agtgtcgaaa
     1141 ggaatagctg cgacttcact cctagcaagc ggacggactg caaaagattg ttcttttccc
     1201 tctttcgttt taatgatcgc ttgttcctgc ccaaccttga gtggttgcgc aagtttgcct
     1261 ttgacgacct gatgatgact ttggcctttg ctgcctgtga gatggtaatc cacgacgtga
     1321 tttttgtcat tcaccacgat ggtaacatta tctccctgtt tcagagtaaa tcccttctct
     1381 tttgcccttt ccattgacag gtaacgggga ctgatttctc ccgtatcacc ggaattgacc
     1441 ttggcctgat ccgctttcac gctttcaacc actccattaa taacccgatg accgggaagc
     1501 agggtaaatt gttggtgctg atcaccatcc tttttccctt gttccatcgt ggagtgaggt
     1561 ttcgagggat cgggagagga cttctctcct cctgacgcag ccgctattcc cactaaaagg
     1621 tacacacacg acccgagaag cacggtggtt ttccagcctg cggcaaattt tttgaacaat
     1681 gacatatgtt ctcctttcga gtagtacgtt tttcaggtaa cggtgccggg ccatcattga
     1741 gatggagcgg aaaccgacac caagcgagag gtaaataaag ttccggatct taatgtctgg
     1801 gagtcacagg catggtccgg tcaacagcat ctaacgccga accacccgaa attgaactca
     1861 tggcacgatc acgctcttgc cggagatcgg aagtttcttt ttgaagggca tctcgctctt
     1921 tcaaaaattg gtcgcggtcc tgaattaagc gggcattctc ttgtttgtct gctccactac
     1981 ccatcttggc actactcgtt tgtccttgaa tcgatttcat gctgtcggag gaatcgtctg
     2041 gactcttgtt ggcctggagt gcctgatcgc gttcttgctg agcttgatcc cgttcatgcc
     2101 gggcttgatc acggctctgc tttgcctgat cccgttcacg ttgcaattgg tcgctccatt
     2161 gaatgtcagc cccggattgc atattggtct ggacatctgg aacagaggtg gaccccgaac
     2221 cgaccggaga agatacctcg ccttgtcctg cgagaacggt tcccggaaga agcatcatag
     2281 cggtggccag taggacgctg ccgaatgttt gaatggaaaa ttttgatgcg aatggtctca
     2341 tgatttttac ctcctactga gttaatagag tggcttctac tttggtaaac cggaagaaga
     2401 aaaacaaaag ctttgaccgt atgggcttct aacaggaaac cgaccagctt cccattgtca
     2461 tgcactcttc aaatcttaca ttgcttcgct ctgctctgtg cacggacaaa tttttaaaca
     2521 gtcatataga tttgtccatg ccgtctcatc aacctgcatc gtctaaagat ttttggtcaa
     2581 tactcatgag ggattcgagc gaaagataaa tttctgcgcg ctgaaggcaa ttcagccgtg
     2641 gcaaaattta ttcctccgtg aagtgaagct aagtagatgg agaggaagga gttgaagggg
     2701 tatgaggagg ggatggggaa ggagagtgtg gagagaggac ataacgggat tcaatggcgg
     2761 ctttgatgtg agccagactt tgcccatgtt ctagcgattc agtggattct attttccgaa
     2821 tctcggcggc caatgcctta tctttaatgc aatcttgggc ccaggaagaa taatcatgcc
     2881 gcttcaaatg atacaaccac gtttcaggat ccaacccttc cgccagttga agaaaaagca
     2941 taagattctg ggctcgaaga ttcagctttc cttccggacc ttgaaagtaa aaactgcgat
     3001 caggtcccaa ttctccttcc gcatacttcc gacgatgacg cctccgctcg ctcctattgg
     3061 ggtgaatccg gaatcgaatc ggcagagccc ctccggaccg tttccagaat atcgcctccc
     3121 ccggttggac gggatggtcc tcgaatgaag gcagagattc ccctaccgaa ttagtgaaag
     3181 cctccaaccg ctggcctgga gattttccaa ttactacgac gcaatcaatt gcggcaaggg
     3241 cgacggcagc catttgatcg ggatgcacgg tgatcatgag cagggaatgg ggtgatgggg
     3301 tgaacaagga gaaagggata tcccattctg ccggcatcag atgatgagtt tcatccagta
     3361 caatccaatg ggggcgtccc gttcggtctc tgaagtcttg aagatcctta aagatgtttt
     3421 taaaaaaaga aggacgttcc tgtatcgcca ggcccaccag attgatgacc aggttcgtat
     3481 ccggattttc cagaatgtcg atgacttcct ttgtgttggg ggtgtgattc atgtcgccca
     3541 ggaccacagc ctgttccaac gattcataat cgccttcggg atcgataata cagcattgat
     3601 aatgtttatc cgtgagacgc tcaatgatgc cggttgagag cgtggatttc ccgcttcccg
     3661 atgagccggc cagaagaagg ttcggaccat acggactgat ccggacttcc tgctccgtct
     3721 ctgtggatcc cagcaccaca taatgccggt gaagggaggg gtcgattgat tgtaagtcgt
     3781 cagtcagaag atgccggata aactccacta ctccgtctcc ttgctctcct tccgtcacga
     3841 tatcggcgcg ttccttgatg gccgggaggg cattcccgac tgccgcggaa cattccacca
     3901 ttcccagaaa ggagagatca ttctcggcat cgccaattcc ggcgacattg tggggtgata
     3961 ggcccatctt ccgtagcgct ttctccaggc cggtcgcttt actgaagccg gcgggaagca
     4021 tcatgacggc ttctttgtta aaaatcagtt gaatatccaa tccaagatca cggaccactt
     4081 ccaatacgac agtttcatgc gggcggacgg tggagacgat atttcgtcca actgaaatag
     4141 gaatgccccg ggaacgaagc cccgtgataa aatcttcaga cggcatatcc gccagtgtga
     4201 tcgtttcttt ggaagaaggt ttgtacaaca caagcccatt ttccgcgaca atccaatcaa
     4261 ataaatgggg gcgaggaaat acattcagta ggtcatccag ttcccggcct gtgaccagaa
     4321 gaagcttgcg attagtggtt aagacttttt ccagacttcg aacggtcgat tcgtgaacac
     4381 gcccatgaag ggcaagagtg ccgtcataat cggtagccag cgctaaatat ctcatataat
     4441 tgtctctggt aaaaataaaa caacttcttg gtaaactatg cggtgcaatt gattaggcat
     4501 attaattatt ccacgaacct gatcgacacg tgttccgaat tgtgtctcaa ttttcttcag
     4561 ccaaaccggg gtgcgcttaa aacattccac ttaacatatc cgtacgatta tttccgatgg
     4621 ctcttgttgt gaggtcataa tccattctct tgtataggtt ccgaagatag aattccctct
     4681 catctgtatc gtcccttttc cttcaaccat cgtggacctt tattgtgggg gaatggccga
     4741 agaacgcctt tccccataaa ctctgttttg atttcacaga tcgggactct cacatcttat
     4801 gaagcagcgg cgcgatccca gaaacgaagc tttcggcacg cctcaagaaa ttgagttgac
     4861 cccttcccct tatcaagggc aatgaagcca gcgtctcgac tctcctgcac tccggccttt
     4921 tgaaacaggg gagtgacggc ttcgacgtaa ccgataaact tcatgtgagc aaaggcatca
     4981 gccacaaaat ccctggcggc aggaaccttg gccagggcca gccctccttc ctcggaaggt
     5041 aaaagtgcta ctgcgtcata cagcaccgat ggcccaccat caattttttg ttccgcttca
     5101 atccacgtcc cgtcgctggc ttccactccg cccacaccgg gggccacaat ctccatgatt
     5161 gccccttccg acttgacggc ggatttgagt gatttaaata attttatatc gactccgtcg
     5221 gtgatgagca ctcccagctt tcgccctttg aaggtattgg gactattgag aagaatgctg
     5281 agcgcgggtg attgctcaag gtcctctcgt gtgggttttg ccgcatccgc aggcttaggc
     5341 atgtctttca ggcgcagacc cttggccact tgtttagcca aatcctggtg aatattcagc
     5401 agatgagaaa ccatacgagt ccgg
//
LOCUS       DATIAL010000265         2858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_801400, whole genome shotgun sequence.
ACCESSION   DATIAL010000265 DATIAL010000000
VERSION     DATIAL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801400"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..579
                     /locus_tag="VLA60_08180"
     CDS             19..579
                     /locus_tag="VLA60_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09377.1"
                     /translation="MRKPSEVTGDRALLIYTLHLTESMGLMSDVKLQQLLFLCELQML
                     GKGLKGLHFEFVRFPYGAFSKDVDNDLLFLRRKERIENFDVTEQAQGVVGLVEKIMKE
                     PEANQQVMEIIHTVVEKYGPMDTSAIMSAVEAVELGPADNPEQKLAIRDISFHTIMLV
                     PSRIEAAEAITLAPAQVKSLNTAMGY"
     gene            complement(704..1030)
                     /locus_tag="VLA60_08185"
     CDS             complement(704..1030)
                     /locus_tag="VLA60_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF09378.1"
                     /translation="MRLHSQQFSVFMFALVLSVGLLFPMSWAEATVNKIDINTASLEQ
                     LEAVKGVGQDIAHNILAYKKDHGAFKSFDDLGKVKGVGKVRLEALRETFTVGSTASPE
                     EASPTK"
     gene            complement(1199..2758)
                     /locus_tag="VLA60_08190"
     CDS             complement(1199..2758)
                     /locus_tag="VLA60_08190"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HSF09379.1"
                     /translation="MSADAALDPLKQTLRQIHHLHDAAAVLSWDQETYMPPGGGAVRA
                     EQLATLQTLAHDQFVSPEMESLLGTFVDLSTGTLHSQHASVLDEPSQALLRETWRDFS
                     RAKKLPSAFVNQLERECSLAQQVWAEARKTNDFQRFLPNLQRVVKLKQQEADYLGYAD
                     SPYNALLDTYEPGSTVAQLRPLFATLRTELIRLLDHIRQSPVQPNTQLLTQSYEHSPQ
                     MDFGRLVLKHMGYDFQRGRLDLSEHPFTTAFHPTDVRVTTRVFETDLPSCLFSCIHEG
                     GHGLYEQGLPAKQYGTPLGEAISLGIHESQSRLWENCVGRSRAFWQYFYPKLQEVFPT
                     QLGQVSAEDFYLAINRVAPSFIRVEADELTYNLHIMVRFEIELDIIEGRLYVDDLPEI
                     WNAKIQEYLGIVPSSDAEGVLQDVHWSFGAFGYFPTYTLGNLYAAMLFRQAHKDLPDL
                     DQAISQGNLLPLKGWLNDKVHRWGRQYTAADLITRVTGQALTPEPFIQDLRQKFGTLY
                     QFPTTVPPSSP"
BASE COUNT          727 a          677 c          782 g          672 t
ORIGIN      
        1 gaaatttagg gagaacctat gagaaaaccc tcagaagtga ccggtgaccg cgctttacta
       61 atttatacgt tgcatttgac cgaatccatg ggtttgatga gtgacgtgaa acttcagcaa
      121 ttattatttc tttgtgaact gcaaatgttg gggaaaggat tgaaagggtt gcattttgaa
      181 ttcgtacgct ttccctatgg ggcctttagc aaggatgtgg ataatgattt gctctttttg
      241 cgtagaaagg aacggataga gaattttgat gtgacagaac aggctcaggg ggttgtaggc
      301 ttggttgaga agatcatgaa ggaacccgaa gccaatcaac aagtcatgga aattattcac
      361 acggtggttg agaaatacgg gcctatggat accagcgcca tcatgagtgc cgtggaggcg
      421 gtggaattag gtcccgctga taatccggag caaaaacttg ccatccggga tatttccttc
      481 cataccatta tgctggtgcc ctcccggatt gaagcggccg aagccatcac gcttgcaccc
      541 gcccaagtaa aatccttaaa tacggcgatg gggtattaag gccccatcga catccggatg
      601 acggtgtatc cttgctctgc aattattgaa ggtaaggaga ccatgagagg cccgaggccg
      661 aacatgggtt cgacctcggt cttgtgaatt gaaaatgctg tggttacttg gtaggcgagg
      721 cttcttcagg cgaagcggtt gagccaacgg taaaggtttc ccgcagcgct tccagtctga
      781 ccttccccac gcctttcacc ttccccagat catcaaacga tttaaaggcg ccatgatcct
      841 ttttgtaggc gaggatgttg tgggcgatat cctgtcccac gcctttgacg gcttcgagtt
      901 gttcaagact ggcagtgtta atgtcaatct tgttcacggt cgcttctgcc caggacatgg
      961 gaaacaacag gccaacggac agaaccagag caaacatgaa gaccgaaaat tgttgtgaat
     1021 gaagcctcac ggaacacact cctttcaaaa tggttgaaac gtgtcgttgc cgttctttcc
     1081 cttcacgggg agaaggccac gaatgaatga gtgaggctag ggagcaaaag gattgaaggg
     1141 caatctgtcg aaggtggtat cttgggagtg tcaaaagccc aataaatgtg aaaatttact
     1201 atggcgaaga cggaggaacg gttgtcggaa attgatacaa ggtgccgaat ttctgtctga
     1261 ggtcttgaat aaatggttca ggagtcaggg cctgcccagt cacgcgggtg attaaatcgg
     1321 ctgccgtata ctgtcgtccc caacgatgca ccttatcatt cagccagcct ttcaatggaa
     1381 gcaagttgcc ttgactgatg gcctggtcga ggtcaggaag gtccttgtgt gcctgccgaa
     1441 agagcatggc tgcatacaga ttcccaagcg tgtatgtggg gaaatagcca aaggccccaa
     1501 aggaccagtg cacgtcctgt agcacacctt ccgcatcgga ggaaggaacg atccccaggt
     1561 attcctggat tttggcgttc cagatttccg gcagatcatc aacgtacaag cggccttcaa
     1621 tgatatccag ctcaatttca aatcgcacca tgatatgcag gttgtacgtc agttcatcgg
     1681 cttcgacccg aataaacgac ggggccaccc ggttaatcgc cagatagaaa tcttctgcac
     1741 tgacctgccc caactgtgtc ggaaacacct cctgaagttt cgggtaaaaa tattgccaaa
     1801 acgcgcggga ccgtcccacg caattttccc ataaacggga ttggctttca tgaataccca
     1861 gagaaatggc ctcacccaat ggagtaccat attgttttgc gggtaaccct tgttcgtaca
     1921 ggccatgccc tccttcgtgg atacagctaa agagacagga ggggagatcc gtttcgaaga
     1981 cgcgggtagt cacccgcaca tcggttgggt gaaacgccgt cgtaaacgga tgctccgaca
     2041 aatccaggcg cccgcgttga aaatcatagc ccatgtgctt gagcacaagc cggccaaagt
     2101 ccatctgtgg ggagtgctca taggattgcg tcaggagttg agtattgggt tggacagggg
     2161 actgtcgtat gtggtctaac agccgaatca gctccgtccg caaggtggca aagagtggac
     2221 gtaattgggc caccgtcgat cctggttcgt aggtgtcgag taacgcatta tagggagaat
     2281 ccgcgtaacc caaataatcc gcctcttgtt gcttgagttt tacgacccgt tgcagattcg
     2341 gcagaaaccg ttggaagtca ttcgtcttcc tggcctccgc ccatacctgt tgcgccaaag
     2401 aacattcccg ctcaagctgg ttgacgaagg ccgacggcaa ttttttcgca cgggaaaagt
     2461 cccgccaggt ctcgcgaagg agagcttgag agggttcgtc caagacagac gcgtgctgag
     2521 aatgaagcgt gccggtggag agatccacaa atgttccaag cagggattcc atctcaggcg
     2581 aaacaaattg gtcatgggcc agcgtttgaa gagtagccag ttgctccgcg cgaaccgctc
     2641 ctcctcccgg aggcatatag gtttcctgat cccaggagag cacggctgcg gcatcatgca
     2701 ggtgatggat ttgtcgcaag gtctgtttta gcggatcaag agcagcatca gcggacatga
     2761 gcggtttcct caaagagtgg tcgtaattgg gccaccatcg atcctggttc gtaggtgtcg
     2821 agtaacgcat tatagggaga atccgcgtaa cccaaata
//
LOCUS       DATIAL010000266         7098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_156997, whole genome shotgun sequence.
ACCESSION   DATIAL010000266 DATIAL010000000
VERSION     DATIAL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156997"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1134
                     /locus_tag="VLA60_08195"
     CDS             235..1134
                     /locus_tag="VLA60_08195"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF09380.1"
                     /translation="MKTPTEKIGFVGVGRMGANMARRLQDMGYPVTTVFDVDSERANS
                     LAKELGCEAVSTPGEVAALSNTIFTVVTDDGAMQDIFSEGHPKSLLKNAEGRLFVNCA
                     TLSPHIHVELEQRVEARGGHALEACMASSIPQAREGTLYLMCGGKPEVFERAKPVLST
                     LSAQLRLIGPAGQAAKVKALVNMVMNINTAGLAEGLGLGDALGLDLTMLREVFSQTGA
                     ASRVLETDGADMQHRDHECYFSVAHAAKDSGIAFALAESVGLTLPLAKATLEQYQRLV
                     KLGKGELDKSGIAELTFKDRCSR"
     gene            complement(1438..1881)
                     /locus_tag="VLA60_08200"
     CDS             complement(1438..1881)
                     /locus_tag="VLA60_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09381.1"
                     /translation="MAVKKAVKSTARKASKKASQVKSAAKKKSATVAKQAKTAAKTGV
                     KKAKTATKQAKSTISKAKKTATKRSANVSSRAKKTVSQLKKEGAQKVASATKTIQKKT
                     RKAKSTVRSAAKQAGKTVGKNVGKALKTVDDLVHQAQEFGKTLLG"
     gene            2159..2761
                     /locus_tag="VLA60_08205"
     CDS             2159..2761
                     /locus_tag="VLA60_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSF09382.1"
                     /translation="MKIGMWSIHLAVLLVGVSGCATFSSSPFSTNEEAQVTAGASFAQ
                     VVQDPSAHIGHILKVGGVVLSAKRLVDRTEVMVLQIPLDSDSVPKWNRTTSKGRFIAT
                     QQPFLDPATLPPGTRLTIIGEVTGQASVRVDEEEKVYPVLAIQALKVWPMIPRNTYGY
                     RPYWGPYPYWGGYWGWGWPYSASSFGYYGRRHGGGKRFRR"
     gene            2858..3157
                     /locus_tag="VLA60_08210"
     CDS             2858..3157
                     /locus_tag="VLA60_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09383.1"
                     /translation="MKKVLSAVFSVLFVVGLAGVSSAGMMDTLKGKADETTQTSTDAH
                     KGMDMTEHAKEHAIEAQSATKEEAGGMMDQLKEGAKGEVNKTKDTANETIDNIGK"
     gene            3705..4469
                     /locus_tag="VLA60_08215"
     CDS             3705..4469
                     /locus_tag="VLA60_08215"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSF09384.1"
                     /translation="MPALLSLLFFAVVQFGVSGLCWAADAPVFGTFALFEVDKDWGNL
                     PLEERQKGGEEAKALLESFKSQVTVDGYWTYGLTTESHFLLRLHSAELKANQQLLTQL
                     RSTGLGRHLDLEYTISGVTKGLNYAPDFPDLLQQLKGAKYEGDPSTYAIMIPIRKNAE
                     WWNLSKEDRVALMKEHTIPTVAYLKTVKRKLYHSTGISDVDFLTIFETNNLVDFNDLV
                     IALRMVREDIFNVQLGEPTIIGTLKSWNDIVELLVK"
     gene            4628..4915
                     /locus_tag="VLA60_08220"
     CDS             4628..4915
                     /locus_tag="VLA60_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09385.1"
                     /translation="MKRVFLMCAGVVFLFGIGSVSFAAGMGDDMTGKTETMKEEGMKK
                     ADDGMGDMKGKGQEMSSDAKGKDMPGNIEAQGKEKMGKMKKKPMKEEPMKK"
     gene            5307..6467
                     /locus_tag="VLA60_08225"
     CDS             5307..6467
                     /locus_tag="VLA60_08225"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HSF09386.1"
                     /translation="MTQPVASLALDSTFPLTNHTVLRKWANVIVFLLCVHFGWMPHAI
                     AVEDSYIAGYAAAVLQHEFNATNASLIVKDGVVTVYAVSLGTLDRTKVQTALEGIPGV
                     KGVEVIEGNVDSVTPQTPPSDAITQEIPKPESKFLPNGLLFDPLHADPRWPHFSVAYR
                     HISEGSEPSKTGSANFGETFAIYRDAAPFDGQWEIAFQGGVFSVFDLDASSKDLVNAD
                     YTAGLLASYRTGSLSGFLRIHHQSSHLGDEFILNNQPNRINLSYEEIDVKLSYELFEW
                     FRVYGGGGILMNRDPNGLGRGTSQFGAELTSPWTLLSGKIRPVAYGDFQINERSNWII
                     NRSLMAGLQFENARIGDRKLQLLMEYYKGASPNGQFYSQRTEWIGIGLHLYY"
     gene            6789..>7098
                     /locus_tag="VLA60_08230"
     CDS             6789..>7098
                     /locus_tag="VLA60_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF09387.1"
                     /translation="MAKSTAEHHSNKNDFLTLYRDMLLIRRFEEKSAEMYALAKIAGF
                     LHLYIGQEAVAVGCMHAIRQDDYVIASYRDHGHCLAKGSDPKKVMAELFGKATGLCKG
                     K"
BASE COUNT         1688 a         1622 c         1924 g         1864 t
ORIGIN      
        1 aggtcggttt atggggtcta tccggactct gctgaggtag ccagggaagc ttgatttcta
       61 acgatgaggc aagtgtcctt gaatgtttgg ttgtgcaggt tcggtgcctt cggtacagtg
      121 aatctccatg catatttccc agggaaaaac tgggaatagt ccgcgaaagg gaagaaatgg
      181 cttatctgca gcgggatctc gttgtgtaat ttctaggaaa agatggaggt ggacgtgaag
      241 acaccaactg aaaaaattgg atttgtgggg gtgggacgga tgggggccaa tatggcccgc
      301 cggcttcagg atatgggtta tccggtgaca acggttttcg atgtggacag cgaaagggcg
      361 aacagtctgg ccaaagaatt aggctgtgag gccgtgtcca ctccgggtga ggtggctgcc
      421 ttatcgaata cgatttttac cgtggtgacg gatgacgggg cgatgcagga cattttttca
      481 gagggtcatc cgaagagtct cttgaagaat gccgaaggtc gattgtttgt gaattgtgcg
      541 acgctttccc ctcacattca cgtggaactc gaacaacgcg tggaagcacg aggggggcat
      601 gctctggagg catgtatggc cagcagcatt ccccaagcgc gtgaaggcac gttatatctc
      661 atgtgtggcg gaaagcctga agtcttcgaa cgagccaaac cggttttaag caccttgagt
      721 gctcagttac gtcttattgg ccccgccggg caagccgcaa aagtgaaggc tctggtcaat
      781 atggtgatga atatcaacac cgccggtttg gcggaggggt tggggcttgg agatgcgctt
      841 gggctggatc tgacgatgtt gcgggaagtg ttcagtcaaa ccggggcggc atcacgggtt
      901 ctggaaaccg atggagccga tatgcaacat cgtgaccacg aatgctattt ttcagtcgcc
      961 cacgccgcca aagattccgg cattgccttt gcgcttgcgg aatccgttgg tctgaccctg
     1021 cccttggcta aagcgacgtt ggagcaatat cagcggttgg tgaagctggg gaaaggcgag
     1081 ttggataaat ccggcattgc ggaactgacc tttaaagacc gctgttcgcg gtaagcgagt
     1141 caaacccgtt tactcttctt ccctcggatt aatgaggccc ttgtcatccc gacccgtgcg
     1201 gcaaagctca gggccaggtt caccgaagga tctcggggag cagtagcgtc ttccactttg
     1261 gcacagattc ttcgccgcag gctcagaatg acaaggtact ttgtgactac ctgagtgaac
     1321 cgaaggatct cgcggagccg tagcatcgtc cacctgggca cagatccttc gccgtggtct
     1381 caggatgaca ggtcggttga agggtattgc gggcatatat ttgacaccga gtgattgcta
     1441 gcccaggagc gttttgccga attcctgtgc ttgatgaacc aaatcatcca cagtttttaa
     1501 cgctttcccc acatttttcc ctaccgtttt tcccgcttgc ttcgctgcgc tgcgcacggt
     1561 gcttttagcc tttcgagttt tcttctgaat agtttttgtc gctgaggcca ctttctgcgc
     1621 cccttccttt ttcaattggc tcacggtttt cttcgcacgc gaggaaacgt tggcggatcg
     1681 ctttgtggcc gttttcttgg ctttggagat ggtgcttttt gcttgtttgg ttgcggtctt
     1741 ggctttcttg actccggttt tggccgctgt cttggcttgc ttggccacgg tcgctgattt
     1801 tttctttgcc gctgacttaa cttgagaggc cttctttgat gcttttctcg ccgtgctctt
     1861 taccgctttt tttacagcca tcgaatgctc cttttgtgta ggtagaggtc attaattgga
     1921 tagtgatagg tgaaatgtaa cctggaacaa tggaaatcac aagtcaaaga cacacctggt
     1981 catgggttta tggcggttcg cggatggaac gcatgtgtcc gttgaatgct ggttggcttc
     2041 tcaatgatta gattcgaacg ttcatcaatc cggcaaataa gaaatcctta ccccctcttt
     2101 cctcttcaaa ggcacgggag agggcgtata atagaatgag ggggtaaaag agggatctat
     2161 gaaaattggg atgtggagta tccatctggc ggttctgttg gttggcgtga gtgggtgcgc
     2221 gacattttcc tcctccccgt tttccacaaa cgaggaagca caggtgacag ccggagcctc
     2281 gtttgctcag gtggttcaag atccctctgc gcatatcgga cacattctca aggtgggtgg
     2341 agttgtgctg tctgcgaaac gtctcgtaga ccggaccgaa gtgatggtgc ttcagatccc
     2401 tttagacagc gattcggttc ccaaatggaa tcggaccacg tcaaaagggc gtttcattgc
     2461 cactcagcag ccctttcttg atccggccac gcttcccccg ggcactcgct taaccattat
     2521 aggagaagtc accggccagg cctccgtccg agtggacgaa gaagaaaaag tctatcctgt
     2581 tcttgccatt caggccttga aagtgtggcc gatgattcca cgaaacacgt atgggtatcg
     2641 tccctattgg gggccttatc cctactgggg tgggtattgg gggtggggtt ggccgtattc
     2701 ggcttcttct ttcggatatt atggtcgtag acatggtggt ggaaaacggt ttcggcgata
     2761 gagatcttcc gtatccctgc tgattgactt cattttgggg aaacgtgcag agtatttttt
     2821 ccccctcatt cacgttcttc cttaaggagg tttttttatg aagaaggtat tatctgcagt
     2881 tttttccgtg ctgtttgtgg tgggtttagc cggagtatcc tccgctggta tgatggatac
     2941 cttaaaaggg aaagccgacg agacaacaca gacttcaact gacgctcata agggcatgga
     3001 catgacggaa catgcaaaag agcatgcgat tgaagcccag agcgccacga aagaagaagc
     3061 cggcggcatg atggatcaac tcaaggaagg ggccaaggga gaagtgaata aaacgaagga
     3121 cacggccaac gaaacaatcg acaatattgg taaataagac acctttctga gttttgggct
     3181 tgggggtgtc caagagtaga acttcagcgt catttgagtg ttgggcggcc tgccgagagt
     3241 gtacctcttt cggtaggccg ttttttttct cgtgtaacga atgaaagaga tgagagacca
     3301 ctatccatgt ttatgcgttt gattggggtt ttccccaaca ttgtctctta gcgggtgggg
     3361 ccggagtcat tcagtcacaa agggaatgtt cgtgagagtc ccgacgtttc tgaaagtcgc
     3421 gtgcttgaaa ggccaatggc cgctttaccg tcacggagca gacccttcgc agacactacc
     3481 gtaccggcat gctgtttgtg gtattccctc cctttctctg ctgtggcctt tctccacaac
     3541 tcaacatttc ttcaaagggt gttttttccc tcgtgcccgt tccaaagtcg gcccgcataa
     3601 gtggtgattc ccgttcaaaa attcctaagg gagactaggg acatttaaac gtttgccatt
     3661 cgcatgtttg gagataagac tcaggtaaga aggagaggaa aaatatgcct gcattactat
     3721 ccctgttgtt cttcgcggtc gtgcagtttg gggtgtctgg tttgtgctgg gccgccgatg
     3781 cccctgtgtt tgggaccttt gccctttttg aggtggataa agattgggga aatttgccgc
     3841 tggaggagag gcagaaaggc ggggaggagg ccaaagctct gctggaatca ttcaaaagtc
     3901 aggtgacggt agatggatat tggacctatg gattgacgac ggaatcccat tttttgcttc
     3961 ggctgcattc ggccgaactc aaagccaacc agcaattact gacccaactt cggagtaccg
     4021 gccttggccg gcatttggat ttggagtaca cgatcagtgg ggtgaccaag gggctgaatt
     4081 atgctccgga ttttcccgat ttgctgcaac aactgaaagg ggcgaaatac gaaggtgatc
     4141 cgtctaccta tgcgattatg atccccattc ggaaaaatgc cgaatggtgg aacctgtcca
     4201 aagaggatcg tgtggcgctg atgaaagaac ataccattcc cacggttgct tatttaaaaa
     4261 cggtgaagcg gaaattgtac cattccaccg ggatttcgga tgtggacttt ctcaccatat
     4321 ttgaaaccaa taatctggtg gatttcaatg acctggttat agcgttgcga atggttcgtg
     4381 aagatatctt caatgttcag ctcggagagc cgacgatcat cggcacattg aaaagctgga
     4441 atgatattgt tgagcttctt gtgaaataaa tctatcgcgc tctgcccatg ggagtcgcat
     4501 cgtgtgctct ggggatcttc agcaacatga tgggatttga caataatcga cattcggagc
     4561 agagtgtatt tcttccccca gggcatttaa caattcaact tgtgcgaatt ttcaaggagg
     4621 tgctcctatg aaacgcgtat ttctgatgtg cgcaggtgtc gtgtttctgt ttgggatcgg
     4681 ttccgtatcg tttgcggccg ggatgggaga cgatatgaca ggcaagaccg aaacgatgaa
     4741 agaagagggc atgaaaaagg ctgatgatgg aatgggggac atgaagggaa aaggccaaga
     4801 aatgtcctcc gacgccaaag gcaaggatat gcctggtaac atagaagccc agggcaaaga
     4861 gaaaatgggt aaaatgaaaa agaaacccat gaaagaggaa ccgatgaaaa agtaatcctc
     4921 attcctttta cgcatttcta gaaggccggc tgtggaatga ctctgcagcc ggccttctta
     4981 ttgggacaca aataggaata aggtcgtcat tgttgaataa ataatcagtc cttgcacgtg
     5041 agaccccctg aatcttttta tttgacaatg tccaaattat gccgcgcacc ctggcgtttt
     5101 gtctgtccag ccaaaaccat tcatcatgct attttgtgag cagggcattt tcctgattga
     5161 ccagccgatg ctccctagcc cgaccactta aaaggaaaat ggtctgtggt gccagctatg
     5221 agaaattccc ggtgttctct gaacgttgag tatatcctcg cgaggttctt atccgtttga
     5281 tctttcggta aggagcgcaa attccaatga cccaaccggt tgcttcactc gccctcgact
     5341 ctacttttcc cttaacgaac cataccgtat tgcggaaatg ggcaaacgtg atcgtgttcc
     5401 tgctctgtgt tcattttgga tggatgcctc acgccatcgc ggtcgaagat tcctacatcg
     5461 ccggctatgc cgccgctgtc ctccaacatg agttcaatgc aaccaatgct tccttaatcg
     5521 taaaagacgg cgtcgtgacc gtctatgccg tttcgctcgg gacgctggat cgaacgaagg
     5581 ttcagaccgc gttggaaggc attccaggtg tcaagggagt agaagttata gagggcaatg
     5641 tcgactccgt gactccccaa actcctccat cggatgctat cacgcaagaa attcccaagc
     5701 ccgagtccaa atttcttccg aatggtcttc tctttgatcc acttcatgcc gatccacgtt
     5761 ggccgcattt ctccgtagcc taccgtcata tttcagaagg ttccgaaccc tcaaaaaccg
     5821 gctcggcaaa ttttggagaa acgttcgcga tctatcggga tgctgcgcca ttcgacggac
     5881 aatgggaaat cgcgttccag gggggagttt tcagcgtgtt tgatttggac gcgtcgtcca
     5941 aagacctcgt caacgccgat tatactgccg gtcttcttgc cagttatcgg accggttccc
     6001 tttccggatt ccttcgcatc catcatcaaa gttcacatct tggtgatgaa ttcatactca
     6061 ataaccagcc gaacagaatc aatctgtcct atgaggaaat tgatgtgaag ttgtcttatg
     6121 aactgttcga atggttccgg gtgtatggcg gcggcgggat actcatgaat cgagacccca
     6181 acgggttagg acgcggcaca agccaattcg gggccgaact aaccagccct tggacgttgt
     6241 tgagcgggaa gattcgtccg gtggcgtatg gggattttca gattaacgag agaagcaatt
     6301 ggatcatcaa ccgttcgtta atggcagggc ttcaattcga aaatgcccgc atcggcgatc
     6361 gcaaacttca acttttaatg gagtattata agggtgcctc acccaacggc cagttttatt
     6421 ctcagcgaac tgagtggatc ggaatcggcc ttcatctgta ttactaaccc caacctaccg
     6481 cggattagaa gtcattggag agaaaaggga aagccccttt ttaccctgat gtcggaatgt
     6541 tccacacgcc cgaaaacatg tgaaatttgg ttcctggatt tttccaaacc aggtcaagct
     6601 gagaaggagt ccaggactgg tccctatttc catggtcgtc aaaagggaag ggaaggtaaa
     6661 aaataatttc aggaaagact ttaaaaattg tatcggcatg cgatctcttg ctacagttct
     6721 tctaatattt caaagacagc actgaagggg aacgaagaca cgcaacgtcg atcaagcccg
     6781 atatttatat ggcgaagagt acagcggaac accattcgaa caagaacgat ttcctgacat
     6841 tgtatcggga tatgctgctc atccgacgct ttgaggaaaa gtcggcggag atgtacgcct
     6901 tagcgaaaat cgcgggcttc ctgcatttat atatcgggca ggaagcggtc gccgtgggat
     6961 gtatgcatgc gattcgccag gatgattatg tcattgcctc ttatcgggat cacggacatt
     7021 gtctggccaa gggatcggat cccaaaaaag tcatggctga attgtttgga aaggccaccg
     7081 gcttgtgcaa aggcaaag
//
LOCUS       DATIAL010000267         2107 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1016278, whole genome shotgun sequence.
ACCESSION   DATIAL010000267 DATIAL010000000
VERSION     DATIAL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016278"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2107)
                     /locus_tag="VLA60_08235"
     CDS             complement(<1..>2107)
                     /locus_tag="VLA60_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSF09388.1"
                     /translation="RLHGLLAECEAEARSEMSVVTSLEEFRQRYHVSTEHMREKIQMA
                     LGDLEKAQIATLHSFAAHVLRLYPLESGVDPNFREDEGTQFLETFQDAWNAWLEQELG
                     AHGSARAQWEEVLNGMSLKDLHAFAFSLCQDHMSLPELEKQVNADALDPVFNIWVQSK
                     QQQICNLLKRYDRAKPRKIEKLLNLAGRIFDRVGQAGWQQDSLLSDSDAALLDSPVGK
                     PPGDWDFSDFQDARQTIQAAHRLLQVNERVLGTVIHLLGPFAGRVRQVFSDKGWIRFD
                     GLLVRVRDLLRDHPMVREQLKGNYQAIMVDEFQDTDPVQYEIMLYLAERPGEHSRYWR
                     AIRLVPGKLFIVGDPKQSIYAFRRADIEAFDQVVDKLIQDGGIVCTLLTNFRSDRAIL
                     AAVNAVFDRLFIPQATVQPPNVPLAVGRSPESCSSMTGVEMCVMANTEGEDDWDADQA
                     TRVEAEWLAGWIEEQLRPGKHWIVEKGVRSPLRPGHIAVLFRKFTSAQVYLEALQRHH
                     IPYMTDGERHFYRRPEVIDMVNVLRVLDDPTDAIALVGILRTLLGGLTDQEIMDVAKF
                     GPLDVRRFRMLESWENPKRPVIQKLFQRLTRLHDHVNRVPISELLDHLFDQLPIMELA
                     AASSHGEQAVVNIWKLRDLMNQQAAVPHLSFSASVDRLVDALLTHPSEAEAPLAEDTL
                     AAVRVLTIHKAKGLEFPVVM"
BASE COUNT          528 a          601 c          497 g          481 t
ORIGIN      
        1 cattacaaca ggaaattcca aacctttagc tttatgaatc gtgagcacac ggactgccgc
       61 caacgtgtcc tcggccagag gagcttccgc ttctgacgga tgagtgagga gggcatcgac
      121 caaacgatcc accgaggcag aaaacgataa atgtggaact gcggcctgtt gattcatgag
      181 gtcacggagt ttccagatat tcacgaccgc ttgttcgcca tggctcgaag ccgcggctaa
      241 ttccataata gggagttgat caaacaggtg atcgagcaat tcggaaatag gaaccctgtt
      301 tacatgatca tgaagccggg tcaggcgctg aaaaagtttc tgaatcaccg ggcgttttgg
      361 attctcccag ctttccaaca tgcgaaatcg ccggacatcc aggggtccga atttggccac
      421 atccataatc tcttgatccg tcagcccccc taacaaggtc cgtaaaattc ccacaagagc
      481 aatggcatcg gtgggatcat ccaatactcg tagcacattg accatatcaa tcacttccgg
      541 acgccgataa aaatgccgct cgccatcggt catgtaggga atgtgatgtc gctgcagcgc
      601 ttcaagatac acctgcgcgc tggtgaattt tctgaacagc accgcgatat gcccgggacg
      661 caagggactt ctcactcctt tctcgacaat ccaatgtttt ccgggtcgca attgttcctc
      721 aatccaccca gccagccatt cggcttccac ccgtgtggcc tggtccgcat cccagtcatc
      781 ttccccttct gtattggcca tgacacacat ttcaacgcct gtcatgctgg aacaggattc
      841 gggacttcgg ccgaccgcca gagggacatt cggaggttgc acagtcgctt gaggaatgaa
      901 taaccggtca aacaccgcat tgaccgctgc aagaattgcc ctgtcgctac gaaaattggt
      961 gaggagcgtg catacaattc caccgtcttg aatcaatttg tccaccacct gatcgaatgc
     1021 ctcgatatcc gcacgcctga atgcatagat tgattgctta ggatctccaa cgataaacag
     1081 ctttcccgga accagacgaa ttgcccgcca ataccggctg tgctcaccgg gacgctcggc
     1141 caagtagagc ataatttcat attgaaccgg atcggtatcc tgaaactcat ccaccataat
     1201 ggcttgataa ttccccttca attgttcccg caccatcgga tgatcgcgca ggagatcacg
     1261 cacacggacc agcaacccgt caaaccggat ccaacccttg tctgaaaaga cctgacggac
     1321 tcgtccggca aacggaccta gcaaatgtat gaccgtgccc aacacccttt cattcacttg
     1381 caggagtcga tgtgcagctt gaatggtttg cctggcgtcc tgaaaatccg aaaaatccca
     1441 gtcccccgga ggcttaccga caggtgaatc caacaaggct gcatcagagt cggacaagag
     1501 agaatcctgt tgccatcccg cttgcccgac gcgatcgaaa atccggccgg cgaggttcag
     1561 taacttctcg atttttcgcg gtttcgctcg atcataccgc tttaataaat tgcagatctg
     1621 ttgctgcttg ctctgcaccc atatgttgaa gaccgggtcc aaggcatcgg cattgacctg
     1681 cttttccagc tcaggaagag acatgtgatc ctgacagagg gaaaatgcaa aagcgtgaag
     1741 atctttcagg ctcatgccat tgaggacctc ctcccactgc gcacgggcag aaccgtgcgc
     1801 cccgagttcc tgttccaacc aggcattcca ggcgtcttga aaggtctcaa gaaattgcgt
     1861 tccttcatct tcccgaaaat tgggatcaac tccgctttcc aggggataga gacgaagaac
     1921 atgcgcggca aaactatgga gtgtggcaat ttgagccttt tcgaggtctc ccagagccat
     1981 ttgaatcttc tccctcatgt gctcagtgga cacatgataa cgttgccgga attcttccag
     2041 actggtgacg accgacatct cactccgggc ttcagcctca cattcagcca gcaatccatg
     2101 caaccgg
//
LOCUS       DATIAL010000268        11927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_587058, whole genome shotgun sequence.
ACCESSION   DATIAL010000268 DATIAL010000000
VERSION     DATIAL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587058"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /gene="valS"
                     /locus_tag="VLA60_08240"
     CDS             <1..1973
                     /gene="valS"
                     /locus_tag="VLA60_08240"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSF09389.1"
                     /translation="TGAVKITPAHDFDDFEAGERHNLPRLAILDFQARMSPSGLQEAQ
                     ADPGLRTILNLKSVQEARAMVIEALEKNELLFKVEDHAMAIGKCYRCKTVVEPYLSPQ
                     WFVKIQPLATPAIQAVETGHIRIIPEGWKNNYLGWMRQIKDWCISRQIWWGHQIPAWY
                     CRTCNQDKLLKTSQVGEVPDDKSNSSDQLTMSCFITPDAQPIVSRTPPQSCPTCGGTN
                     LFQDPDVLDTWFSSALWPFSTLGWPENTEDFKSFYPTGTLVTGLDILFFWVARMIMMG
                     LKFTGQVPFRDVYIHALVRDAEGQKMSKSKGNVIDPLTKMRQYGTDALRFTLASMASP
                     GRDIKLAEERIEGYRNFVTKLWNAARFIHLYADGPRTSIAAEFRPFPDRWILSRLNHT
                     IREVSKAFEDYRFDQAASALYQFIWHEYCDWFIELAKPCLQQENHPDAPATRQTLLES
                     FEIIQRLLHPVMPFITEEIWQSFPHEGPSIMTQPFPTAKPEWVNAEAEEAFLLLQAFV
                     NTARTGRAFLNISSAQTPSIYGASTQVNDTAILAFLVPYIESILRSSVITDQAIPSLR
                     TLQLPSGHFSTIGIPVPNEIDLHEVVKKIQKQIGEKEKEVQRLGSRLSSPEFREKAEP
                     SVIQESEDRRTKLIDELGILNMTEQQLASMTT"
     gene            1973..2887
                     /gene="nadC"
                     /locus_tag="VLA60_08245"
     CDS             1973..2887
                     /gene="nadC"
                     /locus_tag="VLA60_08245"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HSF09390.1"
                     /translation="MGAPSLASPPPLFSMRRTISAALEEDLAYGDLTSTLLIPPTQLA
                     RADIIAKGHMIVAGVAIAREVFQTVDSTIELTTHAGDGTMVRPSTTILSMKGKAQSLL
                     QGERIALNFLQRLSGISTLTHQFCEAVRDYPVALVDTRKTTPGLRALEKWAVRLGGGK
                     NHRFSLHDGILIKDNHLMVMASQRLNITQTCLLARQHAPHGLRICVEVETIAQVRQAL
                     KGKADVLLLDNMTPDHVRQAIDIIQKQALVEVSGGMTLDNVRDMAEAGPDFISIGALT
                     HSAPSMDLSMEILPLRTKRRPQKRKQRA"
     gene            2913..3737
                     /locus_tag="VLA60_08250"
     CDS             2913..3737
                     /locus_tag="VLA60_08250"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HSF09391.1"
                     /translation="MVSSTKLAALLPSKKFGETLYIFDELASTNAFAMEKAKNQARSG
                     TVILADRQTAGRGRLDRSWFSPGKSNIYGSLLFVHETPIQYLGWVPLMAGVAIAQALE
                     QQASIRIDLKWPNDLLIGGRKLGGVLCDSFRNPRQHSCVVIGFGINVNLSQSEFPIEL
                     QTSATSLQIHCHCAMDREELIMKMITSLEKNWENLMSNGPQSYLVEYTQWCVTIGQTI
                     QVQFPDGSQLQGLAHSIGEHGQLRVIPSPSDSNDQSARIRDIHSGEILHLRTTAQP"
     gene            3778..4554
                     /locus_tag="VLA60_08255"
     CDS             3778..4554
                     /locus_tag="VLA60_08255"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HSF09392.1"
                     /translation="MLLAIDIGNSQIVCGVFQESILISHWRLSTDHSKTPDEYSIVLR
                     SLLQFHKILPGDILGCIVSSVVPPLTHIFDMLAQSLFGQQPLIVTSACPHGLILRYSN
                     PEEIGTDRLVNAAAAFARYRRYLIIVDFGTATTFCIVTQQGEYLGGTIAPGLKSAADT
                     LHTKTAKLPKVDLIIPASVIGKDTTSSMQSGIMYGYAGLVDEIVRRIQQEIGESPLVI
                     ATGGLAQTIVPISHTIQEVRPNLTLEGLKLLYDRMTPSCG"
     gene            4668..5330
                     /gene="grpE"
                     /locus_tag="VLA60_08260"
     CDS             4668..5330
                     /gene="grpE"
                     /locus_tag="VLA60_08260"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HSF09393.1"
                     /translation="MSLSSYWSFMTQHSGKGKTMSSTEETTGEVTTEGVSPQAHSTED
                     TSESTSENGGTPEEELQIFQDKYLRLAAEFENYKRRAQRDQSDSIRFGNESLLKNLLP
                     IIDNLERAIQCAKDAGTSGPLLEGVELTHKQFLETVGKVGVRQVSTTGHSFDPAIHQA
                     VTQVESENVEPNTVIEEFQKGYLLHDRILRPAMVSVAKEKSELTEPGSTEESGEEGGI
                     HV"
     gene            5327..7258
                     /gene="dnaK"
                     /locus_tag="VLA60_08265"
     CDS             5327..7258
                     /gene="dnaK"
                     /locus_tag="VLA60_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HSF09394.1"
                     /translation="MSKIIGIDLGTTNSCVAVMSGGDPTVIANSEGGRTTPSVVALTD
                     KDERLVGQIAKRQAITNPENTIYSVKRLMGRKYSSSEVKNAANRLSYKTAEGANGDAH
                     VIIRGKSYSPAEVSAMILQKMKQTAEDYLGEKVTDAVITVPAYFDDSQRQATKDAGKI
                     AGLNVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDVSILEIGEGVFEVKSTNG
                     DTFLGGDDFDLRVMDWLVEEFKKDQGIDLRNDRMALQRLKEAAERAKIELSSSQETEI
                     NLPFITADASGPKHLVLKLTRSKYEQLVNDLIERTIEPCKKALADADMTTSDINEIVL
                     VGGMTRMPKVIERVKQFFGKEPHRGVNPDEVVAIGAAIQGGVLKGDVKDVLLLDVTPL
                     SLGIETLGGIFTKLIERNTTIPTKKSQIFSTAADNQTAVTIRVFQGEREMANDNKLLG
                     QFDLVGLPMAPRGVPQVEVTFDIDANGIVHVSAKDMATQKEQSIKITASSGLSKEEVE
                     RLVKEAQSHTEEDKKKRELVEVKNQADTLVYSTEKNLSEHGDKIEETEKSNITGAVDA
                     LRKAMEGTDLEAIKTAMQNLTTASHKLAEEMYKKASTDASGSGSGPDGGPGTTGGESA
                     GSEEKVVDAEFEEVDKGKN"
     gene            7318..8442
                     /gene="dnaJ"
                     /locus_tag="VLA60_08270"
     CDS             7318..8442
                     /gene="dnaJ"
                     /locus_tag="VLA60_08270"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HSF09395.1"
                     /translation="MAQTQKRDYYEILGVDRNASEEDIKKGFRKLARQHHPDLHADPV
                     QKKAAEEKFKEAGEAYEVLSDSDRRKKYDMFGHAAAGQGGGTDGFGFGGGGFGDMFGD
                     IFEDFFGGGRGQNRAEQGNDLQYNLEISFEEAINGKEAKLKIPRWEVCETCHGTGAKS
                     DQAVKPCSACRGAGQIRLQQGFFTINRTCGQCQGSGQVIADPCKTCRGQRRVHKERLL
                     AVTIPAGVETGMRLRLSHEGEHGMNGGPPGDLYVALNVRPHPHFQRKGQEILYDLRLD
                     FVTATLGGKVEVPTLSGTTMVKIPGGTQHDQILRLKGLGAPGLKSQSRGDQLIRIKIQ
                     IPTKLTPKQHELLSAYAEECGISTDTQGEGLFDKVKNMFD"
     gene            8482..9216
                     /locus_tag="VLA60_08275"
     CDS             8482..9216
                     /locus_tag="VLA60_08275"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HSF09396.1"
                     /translation="MPVFFIHSTQVEDERVTIPDPLFSHLSKSLRMRPGDRLILNDER
                     NRRYHTTIFQITKQAIHSTVLSIQESPHSSTTPITLAHAILKGEKMAWVIQKATELGV
                     HALAPLITERVIPRVSPAHAANYQERWDRIALEAAQQSERWSIPKILPIQTFQDFLQQ
                     KNKGIQIMLAERQEKASLSTIALPSDDPNGITVFIGPEGGWTKEEREIAKNLNVVFAT
                     LGQGILRAETASLASLVILQTRLNYL"
     gene            complement(9241..10245)
                     /locus_tag="VLA60_08280"
     CDS             complement(9241..10245)
                     /locus_tag="VLA60_08280"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HSF09397.1"
                     /translation="MKNLPAWNRMVKDLCVLGVLAGIGLVVNPQPTFPQTTPSALTLQ
                     TSPTKVTFLGVHFLDARKGWIVGAGGTILHTDDGGATWNASPRRTNALLTAVTFADAK
                     QGWVLGQNGTILHTVDGGKKWIPQESGTNGTLYAADFLSPEHGWVVGSRGVILQTEDG
                     GETWADQVSGTTADLFGVHFLNEKHGWAVGALGVILATTDGGKSWGLQTTNNPATFFD
                     IVFTDNVAGWVVGTQGTMFQTLNGGEVWVDHTRPCGSPCIKPADLVNVSFINPLVGRI
                     VGERGTILETHNAGFTWQEIEPLNSETLYAQSFLELSNGFAVGDHGTIIHLNSSPSHP
                     "
     gene            complement(10278..>11927)
                     /locus_tag="VLA60_08285"
     CDS             complement(10278..>11927)
                     /locus_tag="VLA60_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSF09398.1"
                     /translation="PMKRVRSLMSVALGFFLFSSVAHAEMFPSDGPPGTTVTISGEGF
                     GEFQNTQANRVEFQGVSALIQSWEPDFILVKVPLQARSGPVMVINGSSKREAGIFSVT
                     NVRIASLNPSQAEAGSLLTIVGEHFGNTAGSRDPNTMFGVNQVMINGIRAEVRRWRPT
                     KIEVLIPANAKSGDVEVRLASSDPLPDGSCCAPVEYAVSNAVPLTIIPSIAFDPTEGP
                     IGSKVVLSGQGFGESRPEDGRIFFGGKPAIIAQWSDRTIVAHVPLNAKSGSLMLHREG
                     NEREIGTFTVLTSQISGMIPPQGPIGTLVTIKGKNFGVYSEAGGTAYAFDFLSGGNGV
                     EIGGVPAVIHRWLDEQIDVWVPFSAKSGPVIVKRGGATPKIDGTCCERQEIVTLKAGD
                     FTVVQPEIHSYSPKEAGLDEVVTITGKGFGEFLKISEATRLSLNQDAHDWQNYRLGQD
                     VSRSEVLVNGIATHVESWTDTEIRIRVPRRPAFGFGNPEGFEADLTKGELILKRGSWD
                     MLDTGECCTPKKYITIVGGPFTILQRGLPDKDYWNEKGRAQ"
BASE COUNT         3379 a         3062 c         2707 g         2779 t
ORIGIN      
        1 gaaccggagc cgtcaaaatt acccctgcgc atgactttga tgactttgaa gcaggcgaac
       61 gacacaatct tccacgcctg gccatactcg attttcaggc tcgaatgagc ccctcaggac
      121 ttcaagaagc tcaagcagac cccggacttc gaaccatcct caacctcaag tcagtccagg
      181 aggctcgcgc catggtgata gaagccctgg agaagaacga acttctcttc aaggttgaag
      241 atcacgccat ggccatcggg aaatgttatc gatgcaaaac cgttgtcgaa ccctatcttt
      301 caccgcaatg gttcgtcaaa atccaaccgt tggcaactcc tgccatccaa gcggttgaaa
      361 ccggacacat tcgaattatt cccgaaggat ggaaaaacaa ttatctgggc tggatgcgcc
      421 agatcaaaga ttggtgcatt tctcgccaaa tctggtgggg acatcaaatc cccgcctggt
      481 actgtcgaac ctgtaaccag gacaagttac ttaaaactag ccaggttggc gaagtccctg
      541 acgataaaag caattcttcc gaccagctca caatgagctg ctttattacc ccggatgcac
      601 aacccatcgt ctcgagaact ccaccacaat cctgtccaac atgcggcggg accaaccttt
      661 ttcaagaccc tgatgtattg gacacctggt tttcatcggc cctctggcca ttttctaccc
      721 tcgggtggcc ggaaaacaca gaagacttta aatcatttta cccaacggga accctagtca
      781 ccggactgga cattctcttc ttttgggttg ccagaatgat catgatgggc ctgaaattta
      841 cggggcaggt cccattccgc gatgtgtaca ttcatgccct ggttcgagac gccgagggtc
      901 agaagatgag caaatccaaa ggaaatgtga ttgatcccct aaccaaaatg cgacaatatg
      961 gaacggatgc cctgcgattt accctggctt caatggcctc tcctggccgg gacattaaac
     1021 tggccgagga acgtattgaa gggtatcgaa attttgtcac aaaactctgg aatgccgcac
     1081 gctttattca tctctatgca gatggaccac ggacatcaat cgcagccgag ttccgacctt
     1141 ttccagatcg atggattctc agccgactga accatacgat ccgtgaagtg agcaaagcct
     1201 ttgaagacta tcgctttgac caagccgcct ctgctctcta tcaattcatc tggcatgaat
     1261 attgtgattg gttcatcgaa ctcgccaagc cctgcctcca acaggaaaac catcctgatg
     1321 cgcctgccac ccgccagaca ctcttggaat cttttgaaat catccaacgg ctattacacc
     1381 ccgtgatgcc atttattacc gaagaaatat ggcaatcctt cccgcacgaa gggccatcaa
     1441 tcatgacgca accctttccc actgcaaagc cggaatgggt caatgctgaa gccgaagagg
     1501 cttttttact tctccaagcg tttgtcaaca cggcaagaac cgggcgtgcc ttcctgaata
     1561 tttcctccgc tcaaactcct tcgatctatg gagcgagcac ccaggtcaat gacacggcca
     1621 ttctggcctt tcttgtgccc tatatcgagt caattctgcg ctcatcggtc atcacagacc
     1681 aggccatccc atctctccgt acattacaat tgccttctgg tcatttcagt acaattggta
     1741 tccccgttcc caatgagatt gacctccatg aagtggttaa aaaaattcag aaacaaattg
     1801 gagaaaaaga gaaggaagtg cagcgccttg gcagtcggtt atcctctcct gagttcaggg
     1861 agaaagcgga gccttccgtc atccaggaat cagaagaccg ccgtaccaag ctgatcgatg
     1921 agttgggaat tctgaatatg accgaacaac aattggcttc catgacgacc tgatgggtgc
     1981 accttctctc gcctcgcctc ctcctctttt ctccatgcgg cgtacaatca gcgctgcgct
     2041 ggaagaagac cttgcctacg gagacctgac ctcaaccctt ctcattccac caactcaatt
     2101 agctcgcgcc gacatcatcg cgaaaggcca catgatcgtg gcaggcgtgg ccattgccag
     2161 ggaggtgttt caaacagtcg attcaaccat tgaacttacc actcatgctg gcgatggaac
     2221 catggtacgc ccgtcaacca ctatcctgag catgaaaggg aaggcgcaat ccctccttca
     2281 aggcgaacga atcgccctga atttcctcca acggctttcg gggatcagca cgctcaccca
     2341 tcagttctgc gaggctgtcc gtgattatcc agtcgcttta gtggacacac gaaaaacaac
     2401 accgggactc cgggccttag aaaaatgggc cgttcgcctt ggcgggggca aaaatcatcg
     2461 cttctcactt catgatggca ttctcattaa agataatcac ttgatggtga tggcatccca
     2521 acggttgaac atcacccaga cttgcctatt ggccaggcaa catgccccgc atggccttcg
     2581 aatttgcgtg gaagtcgaaa caattgcgca ggtccgacag gccttgaagg gaaaagccga
     2641 tgtcctttta ttggataaca tgactcccga tcatgtcagg caggccatag acatcatcca
     2701 aaagcaggcc ttggtggaag tatcaggggg catgactctg gacaatgttc gagatatggc
     2761 agaagccgga ccggatttca tctcgattgg ggcactcacc cattccgctc catccatgga
     2821 cctttccatg gagatccttc ctctccgaac aaaaagacgt cctcaaaagc gcaaacaacg
     2881 agcctgaatt tttctaaccc cctgactaat cagtggtatc ctccacaaaa ctagctgccc
     2941 ttctcccctc aaaaaagttt ggcgagaccc tttatatttt tgacgaatta gcctcaacca
     3001 atgcgtttgc catggaaaaa gccaagaacc aagcacgatc tggaaccgtc attctggccg
     3061 acagacaaac cgcgggaaga ggtcgattag atcggtcttg gttttcccca ggcaagtcca
     3121 atatttacgg atctctcctt tttgttcatg agacacccat tcaatattta ggatgggtgc
     3181 ctctcatggc cggggtggcc attgcccagg cactcgaaca acaggcatct attcgtatcg
     3241 atctaaagtg gcccaatgac ttattaattg gaggacgtaa attgggtggc gttttatgtg
     3301 attcctttcg gaatccaaga cagcattcat gcgtggtaat cggatttggg attaacgtta
     3361 atctctctca atcggaattt cctatagaac ttcaaaccag tgcgacttct ctgcagatcc
     3421 attgccattg cgctatggac agagaagagc tcatcatgaa gatgatcaca tccttggaaa
     3481 aaaactggga aaacctgatg tccaacggac ctcagtctta tcttgtggaa tacacccaat
     3541 ggtgtgtcac cattggacaa acgattcagg ttcagtttcc tgatggaagc caacttcaag
     3601 ggctggctca ttcaatagga gaacacggcc aactccgagt cattccctcc ccttccgact
     3661 caaacgatca atcagctagg attcgggata ttcattccgg cgaaatcctt catcttcgca
     3721 caaccgcaca accatgaccc atcccttaaa gatggctcaa aaggagtgac cctttttatg
     3781 ctgctagcca tcgacattgg aaactcacag attgtctgtg gcgtttttca agaaagcata
     3841 ctgatctcac actggcgctt atccaccgat cattccaaga ctcctgatga atatagcatt
     3901 gttcttcgct ccctccttca atttcataaa atcctgccag gagacatctt aggctgtatt
     3961 gtctctagtg tggtcccccc tctcacacat attttcgaca tgttggccca atcattattc
     4021 ggacaacaac ctctaatcgt caccagcgca tgtcctcatg ggctcatcct ccgatacagt
     4081 aatccagagg aaatcgggac agaccgtctc gtcaatgcgg ctgcagcatt tgcccgctat
     4141 agacgatatc tcattattgt ggattttggg accgcgacca cattttgtat cgtcacccag
     4201 caaggcgagt acttgggtgg aaccattgcc cctggcttaa aaagcgcagc agatacccta
     4261 cacaccaaaa cagccaagct tccaaaagtt gacctgatta tccccgcatc cgtgattggc
     4321 aaagacacga cctccagtat gcagtcagga atcatgtatg gatatgctgg attggttgat
     4381 gaaatcgtga ggagaattca acaggaaatc ggggaatccc cacttgtcat cgcgacaggc
     4441 ggtttggctc aaacaattgt tccaatttcg cacactattc aggaggttag acccaacctt
     4501 acattagaag gcttaaagct cctttatgat cgcatgaccc cttcctgtgg ataactcctc
     4561 tcaagatttt taaatacttt agcttaacta aaaatcataa gaaatttaag aggatgggtt
     4621 gacttgccag atgaagtgac tatctaactt gccataagca atttgaaatg tctctctctt
     4681 cttactggtc atttatgacg caacattcgg gaaaaggtaa aaccatgtcc tcaacagagg
     4741 aaactactgg agaagtgact acagaaggag tcagtcctca agcccattcg accgaggaca
     4801 catcagaaag tacttctgaa aatggcggaa cgccagaaga agaacttcaa attttccaag
     4861 ataaatattt acgcctggcc gcagaatttg aaaactacaa acgccgagct cagcgggatc
     4921 agagcgattc tattcgattt ggcaatgaat cattattgaa aaatttactt cctattattg
     4981 ataaccttga gcgagccatt caatgtgcaa aagatgcagg aacgagtggc ccattactgg
     5041 agggggtaga acttacccat aaacaatttc tggaaaccgt gggcaaagtt ggcgttcgac
     5101 aagtaagcac cacaggacac tcttttgatc ctgctattca tcaagcagtg acccaggtag
     5161 aatctgaaaa tgtggagccg aacactgtca tcgaggaatt tcaaaaagga tatttattgc
     5221 acgatcgcat tttacgtccg gctatggtaa gcgtggcaaa agaaaaatca gaactgacag
     5281 aacctggttc gactgaagaa tctggtgaag agggaggaat acacgtatga gcaaaattat
     5341 tgggattgac ttaggaacaa caaattcttg cgtggcggtg atgagtggtg gagacccaac
     5401 ggtcattgcc aattcagaag gcgggcggac caccccttcg gttgtggctt tgaccgacaa
     5461 agacgaaaga ttggtcggac agattgccaa acggcaagcc ataaccaacc cggaaaacac
     5521 tatttattcg gtgaaacgtc tgatgggaag aaagtattct tcttccgagg tcaaaaatgc
     5581 cgcaaaccgg ctttcctata agacagccga aggtgctaac ggggatgccc acgtcatcat
     5641 tcgagggaaa agctacagtc cagcagaagt ttctgccatg atccttcaaa aaatgaagca
     5701 aacggctgaa gattatttag gggaaaaggt gacagatgct gtcattacgg tcccagccta
     5761 ttttgatgat agccaacgcc aggcgacgaa ggatgcagga aagatcgcgg gcttaaatgt
     5821 cttacgcatc attaatgagc cgactgcagc gtcactcgcc tatggactcg acaagaaaaa
     5881 agatgaacgt atcgcggtct acgatctcgg tggtggaacc tttgacgttt ccattctaga
     5941 aatcggagaa ggagtctttg aagtcaaatc cacaaatggt gatacctttt tgggcggaga
     6001 cgactttgat cttcgggtca tggattggct ggttgaagaa ttcaaaaaag atcaaggaat
     6061 cgatttacgg aatgatcgaa tggccctgca acgactgaaa gaagcagccg aacgggcaaa
     6121 aatcgagctt tcctcctcac aagaaaccga aataaactta cccttcatca ccgccgatgc
     6181 cagtggacca aaacatttgg tacttaaact caccaggtca aaatacgagc aattggtgaa
     6241 tgatttaatt gagcgaacaa tcgaaccttg caaaaaagct cttgccgatg ctgatatgac
     6301 caccagcgac atcaatgaaa tcgtcttggt aggtggaatg actcggatgc caaaagtaat
     6361 tgaacgagtt aagcaatttt tcggcaaaga accccaccgt ggcgtgaacc ctgacgaagt
     6421 cgtggcaatc ggtgcggcca ttcagggtgg agtcctcaaa ggagatgtaa aggatgtctt
     6481 gttattagat gtcaccccgc tttcactcgg catcgaaaca ttagggggaa tcttcacgaa
     6541 actcatcgaa cgcaacacca ccatccccac caaaaagagt caaatttttt caaccgccgc
     6601 cgacaaccaa actgccgtga ccatcagagt cttccaaggt gagcgggaaa tggccaatga
     6661 caataaacta ttgggacagt tcgatttagt gggccttccg atggcaccaa gaggtgttcc
     6721 gcaagttgaa gtcacatttg atatcgacgc taacggcatt gtgcatgtct cagccaaaga
     6781 tatggccaca caaaaagaac aatcaatcaa gatcacggca tcgagtggat taagcaagga
     6841 agaagttgag cgcctggtta aggaagctca aagtcatacc gaagaagaca aaaagaagcg
     6901 ggaattggtc gaagtcaaga atcaagccga tacccttgtt tatagtaccg aaaagaattt
     6961 gagcgagcat ggagataaga ttgaggaaac ggagaaatcc aacattacgg gggcggttga
     7021 cgcacttcgt aaagctatgg agggaacgga cctcgaagca attaaaaccg ccatgcaaaa
     7081 cctcaccacc gcctctcaca aattagcaga ggaaatgtac aaaaaggctt ctacggatgc
     7141 ctctggcagc ggctctgggc cagatggcgg cccaggaacc actggcggtg aaagcgccgg
     7201 cagtgaggaa aaagttgtag atgcagaatt tgaagaagtt gataaaggca aaaattaaaa
     7261 acttttgaac agattaatgc acataggcat atattattcc acctttggac aacgctgatg
     7321 gcacagaccc aaaaacgaga ttattatgaa attctcgggg tggaccgcaa tgcctcggaa
     7381 gaagacatta aaaaaggctt tagaaaactt gccaggcaac atcacccgga cttgcatgcc
     7441 gacccagttc agaaaaaggc ggctgaggaa aagttcaagg aagccgggga agcctatgaa
     7501 gtactcagcg attcagatcg ccgaaaaaaa tatgacatgt ttggtcatgc agcggctggc
     7561 cagggaggag gaaccgatgg tttcgggttt ggaggtggcg gcttcggaga tatgtttggg
     7621 gatatctttg aagacttttt cgggggaggc agaggacaaa atcgagccga acagggcaat
     7681 gatcttcaat ataacttaga aatttccttt gaagaggcca ttaacggcaa ggaagccaaa
     7741 ctcaaaattc ctcgttggga agtctgtgaa acctgccatg gaactggcgc gaaatccgat
     7801 caagccgtca aaccctgttc cgcatgccgt ggagccggac aaatacggtt acaacaaggg
     7861 tttttcacca ttaatcgaac ctgtgggcaa tgccaaggat caggtcaagt cattgctgac
     7921 ccgtgcaaga catgtagagg acaaagacgc gttcacaagg aacggctctt agcagtcacc
     7981 attccagctg gagtcgaaac cggcatgaga cttcgacttt cccatgaagg cgaacatggc
     8041 atgaatggcg gacctcccgg agatttgtat gtggcactca acgtacggcc tcatccgcac
     8101 tttcaacgga aaggccaaga gatactctat gaccttcgct tggattttgt aaccgcgacg
     8161 cttggaggaa aagtcgaagt gcctacatta agtggaacca ccatggtgaa aatccctgga
     8221 gggacccaac atgatcaaat tctccgcctc aaagggttgg gcgctcccgg tcttaagtcc
     8281 caatctcgtg gcgatcaact gattcggatt aaaattcaaa ttcctacaaa actgactcca
     8341 aaacaacacg agctcctttc cgcttatgcc gaggagtgtg gaatatctac ggacacgcaa
     8401 ggagaaggac tgttcgataa agttaaaaac atgtttgatt aacctgcctt ttttactccc
     8461 tctccatcct cacggaaaca gatgcccgtc tttttcatcc attcaaccca ggttgaggat
     8521 gaaagggtca cgatccccga ccccttattc agccatcttt caaaaagttt acgaatgaga
     8581 ccaggagatc ggctgattct taatgatgag cgaaatcgtc gataccatac aactattttt
     8641 cagataacca aacaggctat ccactcgacg gttctgagta ttcaagaatc cccgcactcc
     8701 tccactaccc ccatcacctt ggcacacgca attttaaaag gagagaaaat ggcctgggtc
     8761 atccaaaagg ccactgaact tggagtccat gcgcttgcac cactgattac cgaaagagtc
     8821 attccacgcg tgtctccagc acacgcagcg aattatcagg aacgatggga tcgcattgcc
     8881 ttggaagcgg cacagcaaag tgagcgatgg agcatcccca agatacttcc catccagacc
     8941 tttcaggatt ttctacagca gaaaaataaa ggaatacaaa tcatgttggc tgagcggcag
     9001 gagaaggctt ctctatcgac catagcctta ccgtctgatg atccaaacgg aattacggtg
     9061 ttcatcggac cagaaggagg ttggacgaaa gaggaacgag agatagccaa aaacctgaat
     9121 gtggtttttg ccacgttggg acagggaatt ttgcgagcag aaacagccag cctggcttca
     9181 ttagtcattc tacagaccag actcaactat ctgtgaggaa tacaacggaa gcgcccagca
     9241 ctagggatga gatggcgaag aattcaaatg tatgatcgtc ccgtggtctc ccacagcaaa
     9301 cccattggaa agttcaagga atgattgagc gtacagggtt tcagagttta ggggttcgat
     9361 ttcctgccat gtaaagccgg cgttatgagt ctctaaaatg gtcccccgct caccaacaat
     9421 tcgaccaacc agtgggttta taaaactaac attcaccaaa tcggcgggtt taatgcatgg
     9481 acttccacag ggtcgagtgt gatcaaccca tacttcgcct ccattcagcg tttggaacat
     9541 agttccctgc gtcccaacaa cccacccagc cacattatcc gtaaacacaa tatcaaaaaa
     9601 tgtggcggga ttattggtgg tctggagccc ccaactcttt cccccgtcag tcgtcgccaa
     9661 gatgacacca agggcaccaa cggcccaacc atgtttttca ttaagaaaat gaacaccaaa
     9721 gagatcggct gtcgtcccac tgacctgatc agcccaggtc tctcccccat cttccgtctg
     9781 taaaatgacg ccacgggaac cgactaccca tccatgttca ggtgaaagaa aatcagccgc
     9841 gtagagcgtt ccatttgtcc cactctcctg cggaatccac tttttcccac catctacagt
     9901 atgcaatatc gttccgtttt gtccaagaac ccatccctgc tttgcatccg caaatgtcac
     9961 cgcagtcaat aacgcattgg tgcgtcgagg gctcgcattc caggtagctc caccgtcgtc
    10021 ggtgtgaaga atcgttcctc ctgctcccac gatccatccc ttcctggcat ccagaaaatg
    10081 cacaccaaga aaggtcacct tggtaggact ggtctgaagt gtgagagcag acggagtggt
    10141 ttgaggaaag gttggctgag ggttcaccac taagcctatg ccagcaagga ctcccaacac
    10201 acacagatct ttcaccattc gattccaggc agggagattt ttcattagat catgtttccc
    10261 aattatgcca gaattcatta ctgtgcccgt cctttttcat tccaataatc tttgtcagga
    10321 aggcctctct gtaaaatcgt aaatggcccc cccacgatag taatatactt ttttggcgta
    10381 cagcattcgc cggtatcgag catatcccat gacccacgct tcaaaatcag ttccccttta
    10441 gtgagatcag cctcaaaacc ttctggattt ccaaaaccaa aggccggacg gcgagggaca
    10501 cggatcctaa tctccgtatc ggtccatgac tcgacatgag ttgcaatgcc attcaccagc
    10561 acctcactgc gagacacatc ctgccctaat cggtaatttt gccagtcatg ggcatcctga
    10621 ttgagagata aacgagtggc ttcggatatt ttcagaaatt caccaaatcc cttaccagta
    10681 atagtcacga cttcgtccaa gccggcttcc ttaggggaat aggagtgaat ttccggctga
    10741 acaacagtaa aatctccggc ctttagtgtc acaatctctt gtcgttcaca acaggtgcca
    10801 tcaattttag gagtagctcc tccacgtttc acaatcaccg gcccgctttt tgcgctgaag
    10861 ggcacccata catcgatttg ttcatcaagc caacggtgaa tgacagccgg cactcccccg
    10921 atctccaccc cgttgccccc ggaaagaaaa tcaaaagcat atgccgtgcc gccggcttct
    10981 gaatatacac cgaaattttt tcccttaatc gtcacgagtg tcccaattgg tccttgaggg
    11041 ggaatcattc ctgatatctg actagtcaat acggtaaatg ttccaatttc acgctcattt
    11101 ccttctcgat ggagcatcag ggaacctgat ttggcattta acggcacatg ggccacgatg
    11161 gttcgatcgg accactgcgc aatgatcgca ggctttcctc caaaaaatat tcgtccatcc
    11221 tcagggcggc tttctccaaa cccctgaccc gacagtacga ctttactccc gattgggccc
    11281 tcagtgggat caaaggcaat agaaggaatt atcgtcagag ggacggcatt actgaccgca
    11341 tactcaacag gagcacaaca cgatccatcc ggtagtggat cgcttgacgc caagcgcact
    11401 tccacatcac cggacttggc attagccgga atgagaactt caattttggt aggccgccac
    11461 cgtcgcacct ccgcacgaat cccattgatc atcacttgat tgaccccgaa catggtattg
    11521 ggatcacgag atccagccgt attgccgaaa tgttcaccaa caatcgtgag caaggaaccc
    11581 gcttcggctt gcgaaggatt caagctcgca atacggacat tggtaacgga aaaaattccg
    11641 gcttctcttt tggacgatcc gttgataacc atcacagggc cggaacgagc ctgcaaaggc
    11701 accttgacga gaatgaaatc aggttcccag gattgaatga gcgccgagac accctgaaat
    11761 tccactcgat tagcctgcgt attttggaat tcaccaaacc cttccccact aatggtcact
    11821 gtcgtgccag gaggtccatc ggaaggaaac atttcagcat gggccactga tgaaaaaaga
    11881 aaaaaaccta aggcaacgct catgagactt cgaactcgtt tcatagg
//
LOCUS       DATIAL010000269         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_49293, whole genome shotgun sequence.
ACCESSION   DATIAL010000269 DATIAL010000000
VERSION     DATIAL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49293"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VLA60_08290"
     CDS             <1..182
                     /locus_tag="VLA60_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09399.1"
                     /translation="FATTVGFDCNGPFFLGWAFFTLDAIGSSSYDQWVKRAVINLLLN
                     ETILTQYRTISSSPW"
     gene            complement(686..1072)
                     /locus_tag="VLA60_08295"
     CDS             complement(686..1072)
                     /locus_tag="VLA60_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09400.1"
                     /translation="MKDLADLLEEVHTIVQSIREEKMMLEDRLKSLENDLSTMEKAKK
                     SIACDLQETKERLGRELQATKEKLDQVHPAIQSLQNELQNVEVQNNLLVMDLKQKDEE
                     IQALELRLREYTSLTASSFRADKPVG"
     gene            1375..1833
                     /locus_tag="VLA60_08300"
     CDS             1375..1833
                     /locus_tag="VLA60_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF09401.1"
                     /translation="MNKFPIKVTYGIMATIELAKQDRSIPLQAKIIAQRQGIPSRFIE
                     QILQHLKQAGIIRSLRGAQGGYTLAQHPSQISLADLVNSMNGWATEPLLQHGHTNGHS
                     ESRQVPNVLLSNIWQQVQEAEQAVLRGISLQKLVEQYQTLETQRCLMYHI"
     gene            1935..>2916
                     /locus_tag="VLA60_08305"
     CDS             1935..>2916
                     /locus_tag="VLA60_08305"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HSF09402.1"
                     /translation="MSTSPVTSHPPIHTVPIPQEILEEIETFEDEVNRLQSGDTPTDV
                     FKPFRLQYGIYGQRQADVQMVRIKIPFGGLNANQLRQVADIADTFATGVGHVTTRQDI
                     QLHFVPLRHVGTIMRKLAEVGLTTREACANTVRNVTACALAGVCPGEVFDVTPYAKTV
                     AYHLLRNPLNQSLPRKFKIAFSGCKQDCALTPIHDVGLLAARNPEGVPGFKMVVGGGL
                     GSTPRLAKVLHEFVPMGELIPAIEAMLKVFDNLGNRKNRSKARMKFVIDKLGFEEFSQ
                     RWKETYDAMLQSGATKLGLETVPYPDDPPLVMPTKSGNGTSTQGNGNSHPQ"
BASE COUNT          735 a          755 c          643 g          783 t
ORIGIN      
        1 tttttgcaac gaccgttggc tttgattgta acggtccctt cttcttaggc tgggcttttt
       61 tcactttgga tgccatagga tcctcctctt atgaccagtg ggtaaaacgt gccgtgataa
      121 atcttttgct gaatgaaact atcctgactc aatacaggac aatctcctca tcgccctggt
      181 aatgacggac cattgtacaa tgaataagag gtttcattcc tccttttttc aaggaagcgg
      241 gccacatggc ccctatcctt gcggaggaac ggaacgcgta tagggttgat gccctcgaat
      301 cctttgagca gactatcctt tttaccgcca cctcattgga tctcttctga cggttaattc
      361 tgatgagcaa cgccagaaca cattctgagg ggcctaacca accaggcatt tcccatctgg
      421 ctcacacctt aatgcccaaa cgaatgctca gacttgggat ttcaacccta ggtttgttcc
      481 tatttatcgt tcaccttccc atatctggca ttggcaaagt ggagggatcc ccccccttct
      541 tagtccttta aaggcgccat cataccagga aggaaaatgc tgactggggt tatgaattcg
      601 actcaggaag gaaccaacgc tagatcagag ttttaagaca tttctccaga gtcggaggat
      661 ttgagttgaa aggcattccc ggagcttagc cgacaggctt gtcagcgcgg aaagatgaag
      721 cagtcaagga agtgtattca cgaaggcgca attctaatgc ttgtatttct tcgtctttct
      781 gtttgagatc cataaccaga agattattct gtacttcgac attctgcagc tcattttgca
      841 aactttgtat tgccggatgc acctgatcca atttttcctt tgtagcttgt agttcccgtc
      901 cgagtctttc cttcgtttcc tgtagatcac aagcaatgga ctttttcgct ttctccatgg
      961 tcgataagtc gttctccaac gatttcagcc gatcttcaag catcatcttt tcttcccgga
     1021 tagactggac gatggtatgg acttcctcaa gcagatcagc aaggtctttc atctcattgg
     1081 tcctctcaca gctcccaaac attgcaaccg actttatccg gggctttccc ccagtcatgg
     1141 tatcggacta ttttttccca atatttaatg gccctacgat tttgttgttc agtttttttt
     1201 gtttgaatca atggctccct gatgataaag ccctcccgaa gccaatggtt caggcacccg
     1261 tttttgaccg gcacatcttt ttttgagtaa aagctcgcct catatttctt gacaaatgtt
     1321 ttctgcaaat tttatattgc acattattcc tatcgtttta gtatgaatta aaatatgaac
     1381 aagtttccga tcaaagtcac ctatggaatt atggccacaa tcgagctagc aaagcaggac
     1441 agatccattc cgcttcaagc caaaatcatc gctcagcgac aggggatccc ctctcgcttt
     1501 attgaacaaa tccttcagca cttgaaacaa gccgggatta ttaggagcct tcgaggagca
     1561 caaggagggt ataccctggc acaacacccc agtcagattt cccttgcgga tttagtcaat
     1621 tcaatgaatg ggtgggcgac agaaccattg ctccaacatg ggcacaccaa cggccattcg
     1681 gaaagccgac aggttccgaa cgtcttactt tcaaatattt ggcaacaggt tcaagaggcg
     1741 gagcaggcag ttctgcgggg catttcccta caaaaattgg tcgaacagta tcaaaccctc
     1801 gaaacccagc gctgcctgat gtatcatatt tagctttggc aaaggacgga ccattccagg
     1861 gtcgaacaga aattagggca tttttaagcc aaccctagaa atttacccct ctatacggga
     1921 tttggagaat tatcatgagc acctcaccgg tcacaagcca ccctcctatt cacacggttc
     1981 cgatacccca ggagattctt gaagaaatag aaactttcga agatgaggtc aatcgcctgc
     2041 aatccgggga cactccaacc gacgtgttca aaccgtttcg actccaatac ggtatttatg
     2101 gtcaacgcca ggccgatgtc caaatggtcc ggatcaagat tccctttggt ggtctgaacg
     2161 ccaatcaact acgccaagtg gcagacattg cggatacctt tgcgaccggc gtgggccatg
     2221 tcacgacgcg gcaggatatt caactgcact ttgttcccct ccgccacgtt ggcaccatca
     2281 tgcgaaaact ggcggaagtt ggactcacca cgcgggaagc ctgcgccaat accgtccgaa
     2341 atgtcacagc ctgtgcgttg gcaggagtat gccccggcga ggtgtttgac gtgaccccct
     2401 acgcgaaaac cgtggcttat catttattac ggaatcctct caatcagagt ctccctcgca
     2461 aatttaaaat tgccttttcc ggctgtaagc aggactgtgc cctcactccc atccacgatg
     2521 tcgggcttct ggctgctcgc aatcccgaag gggttccggg attcaaaatg gtcgttggag
     2581 gcggattggg gtccactccg cgtctcgcca aagtacttca tgaattcgtt cccatgggag
     2641 aattgattcc cgccatcgaa gccatgctca aggtgtttga taatttaggt aatcgaaaaa
     2701 atcgaagcaa agcccggatg aaatttgtca ttgacaaatt agggtttgag gaattctctc
     2761 aacgatggaa agagacctac gacgccatgt tgcaatccgg cgccaccaaa cttggattgg
     2821 aaacggttcc gtatcctgat gatcctccct tggtgatgcc gacgaaatcc gggaacggca
     2881 cctccaccca aggcaatggg aacagccacc ctcaaa
//
LOCUS       DATIAL010000270         3516 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1124379, whole genome shotgun sequence.
ACCESSION   DATIAL010000270 DATIAL010000000
VERSION     DATIAL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124379"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            559..1947
                     /gene="ffh"
                     /locus_tag="VLA60_08310"
     CDS             559..1947
                     /gene="ffh"
                     /locus_tag="VLA60_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HSF09403.1"
                     /translation="MFTTLTEKIEKVFKQLRGQAVLTEQNITDALKEVRLALLEADVN
                     FKIVKDFIEKVRIKAVGQEVLKSLTPAHQVVKVVWEELRDLLGHEQSALHLSSQPPTV
                     IMMVGLQGSGKTTTTGKLAHYFKTEGKRVLLVAADPRRPAAGDQLTSLGTDLDIPVHR
                     GTNEGQPGAQAVQTCRDGVARGRDHGYDVVILDTGGRLQIDEELMQELVDIQTGVKPQ
                     EVLLIADSMTGQEAVAVAERFNQALGLTGVILTKVEGDARGGAVLSIRAATGQPIKFL
                     GIGEKLDALEPFYPDRMASRILGMGDVLTLIEKAQENFSQEQAVALQKKVSSNTLTLE
                     DFRDQIKQVNKLGSFDQILDMLPGGQKIKTMMGSGAAGNAQVPEKEMKRVVAIIDSMT
                     PRERRDHTILNGNRKKRVAKGSGTSVPEVNRLIKQFLDARRMMKSLVGGQMGMGKGKK
                     RGKLIRRAIHAR"
     gene            2009..2287
                     /gene="rpsP"
                     /locus_tag="VLA60_08315"
     CDS             2009..2287
                     /gene="rpsP"
                     /locus_tag="VLA60_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HSF09404.1"
                     /translation="MAVHLRLTRVGRHKRPFYRVVAADARMPRDGRFLEVIGTYDPLK
                     ASDKASFKEDRVLNWLQNGAQPTDSVRGLLRQTGILKAFRESKQAPAK"
     gene            2378..2920
                     /gene="rimM"
                     /locus_tag="VLA60_08320"
     CDS             2378..2920
                     /gene="rimM"
                     /locus_tag="VLA60_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HSF09405.1"
                     /translation="MDAPEPSRSSMVTIGRILKPFGIHGEVRIESLSDVPGRFEGLET
                     VTLALPDGRILETLVTSVRQINQGVILGLSAFSTPETAALYRGAWIKIPENQNLPRDP
                     DTFYQFELIGLRVEDAEGQPLGTLEEVLEYPQHHVFVIRNEDREILVPASRRTIAMVD
                     LPHKVLRLTSREWWDTTYAL"
     gene            2910..>3516
                     /gene="trmD"
                     /locus_tag="VLA60_08325"
     CDS             2910..>3516
                     /gene="trmD"
                     /locus_tag="VLA60_08325"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HSF09406.1"
                     /translation="MRCDILTLFPELIHSVSSQSIMKRAQDKGLLTLNVHNIRDYTHD
                     PHRTADDTPYGGGGGMVMKAEPIFQAIDRITPDLDEIRIIIPSPQGIRFSHGLANDLS
                     QDTRPLLFICGHYEGIDERVRTHLRVEEISLGDYVLTGGELAALVMIDAAMRLIPGVL
                     GDPHSAQRESFVESLLDFPHFTKPIEIRGMSVPDILMSGNHE"
BASE COUNT          899 a          839 c          940 g          838 t
ORIGIN      
        1 accggacttc ctgaatggcc accggtcgac cgagttgctc cgaaagcatg ggtggaacct
       61 tcgtggtaat cgcccaggga accactaggt aacccacgag agtatagagt aggaacaatc
      121 caagaagaac atacagaaga attttgccat tttttttcat gatttggggt ttatactcat
      181 gaaagaaaac ggaatagaga agatacgatc aatgaaaacc atttaaaatt tgacgcgagc
      241 cactaaacta ataggataaa ctcgattagc tgggcgtgtc ggttcatgat aacggtctga
      301 ttctttgaga gctagcctga tccaccctgt cttgttactc ctcctccatc cctgccaccc
      361 caaattgatt ttaatatagg ccctgctcgc ctatcacggg gattacacaa agctcatgaa
      421 tccaaaagat ttcaggtgga taaccaatcc cggttttgcc ccaaccaaaa ggtggtactc
      481 aacttgacat gcctgatgtt tgttcactat tgtcttgggt ttgcttaaaa tggtgtattt
      541 tccattggat cccttttcat gtttaccacg ctgacagaga aaatcgaaaa agttttcaaa
      601 cagttacgcg gccaggccgt tctaactgaa caaaatatta ccgatgccct gaaagaggtg
      661 cgcctggcgc tgttggaagc cgacgtcaat ttcaaaattg ttaaagactt tattgagaag
      721 gttcggataa aggccgtcgg gcaggaagtc ctgaaaagtc tcacacccgc ccatcaagtg
      781 gtaaaagttg tctgggagga gctgcgggac cttctcggcc atgaacaaag cgcccttcat
      841 ctctcttcac agccccctac ggtcatcatg atggtcgggt tgcaaggctc aggcaagacc
      901 accaccaccg ggaaactcgc tcattatttt aaaactgaag gcaagcgggt cttgttggtc
      961 gcagccgatc cccgtcgccc tgccgcagga gatcagctga cctcactcgg gactgacctg
     1021 gatattccag tccaccgtgg gacgaacgag ggtcaacctg gcgcccaggc cgttcaaacc
     1081 tgtcgggatg gagtcgcccg agggcgggat cacggctacg atgtggtgat cttagatacc
     1141 ggcggtcgac tgcaaatcga tgaagaattg atgcaggaat tagtcgatat ccaaacgggc
     1201 gttaagccac aggaagtctt gttaatcgcc gactccatga ccggccagga agcggttgct
     1261 gtcgccgaac gctttaatca ggccctcggt ctcactggcg tcattttaac gaaggtcgag
     1321 ggcgatgccc gtggcggagc ggtgctctcg atccgggcgg cgacaggcca acctattaaa
     1381 ttcctaggaa tcggggaaaa gctggatgcg ttagagccct tttatccgga ccggatggcc
     1441 tcccgtattc tcggtatggg tgacgtgctg accttgatcg agaaagccca ggagaacttt
     1501 tcgcaggaac aagccgtcgc attgcaaaaa aaggtttcga gtaataccct gacgttggaa
     1561 gattttcgtg atcagatcaa acaggtgaat aaattagggt cgttcgatca gattttagac
     1621 atgctcccgg gcggacaaaa aattaaaacg atgatgggtt ccggtgccgc cgggaatgct
     1681 caggtacccg aaaaagaaat gaaacgggtg gtggcgatta tcgattcgat gaccccgcgc
     1741 gaaagacggg atcatacgat tttaaatgga aatcggaaaa agagggtggc caaaggcagc
     1801 ggaacatccg ttccagaggt caatcggctc attaagcaat ttttggatgc ccgacgcatg
     1861 atgaagtccc tggtcggagg gcaaatgggg atgggaaaag gaaaaaagcg cgggaagctg
     1921 atccgccggg ctatccatgc ccggtaagaa tattgaagtc tccctgagct ttttcattca
     1981 ttcatattta cgatagagga gattgccagt ggcagtacat ttgcgactta cacgcgtggg
     2041 ccggcataaa cggccatttt atcgagtagt ggcagccgat gcccgcatgc cgcgggatgg
     2101 ccggttcctt gaggtcattg ggacctatga ccccctcaag gcaagcgata aggcgtcgtt
     2161 taaagaagat cgggtgttga attggcttca aaatggggct cagcccactg attccgtaag
     2221 ggggttatta cgacaaaccg gtatcttgaa ggcctttcgg gaatccaaac aagccccagc
     2281 aaaataatat cggcatgtca gggtcatggg agaggttttg ccctgttgta ttctgcaaag
     2341 agtcctttgg ggatgccacc gtactcatgg ctaaattatg gatgctcctg agccatcgcg
     2401 gtcttccatg gtgaccattg gccggattct caagccgttc ggcatccatg gggaagtacg
     2461 tattgaatcg ctgtcggatg ttcccgggcg gtttgaggga ttagagacgg tgacactggc
     2521 ccttccagat gggaggattc tggaaaccct ggtgacttcg gttcggcaga tcaaccaggg
     2581 ggtgattttg ggactctcag cgttttcgac accggaaacc gcggccttgt accgaggggc
     2641 ctggatcaag attccagaaa accaaaacct tcccagggac ccagatacgt tttatcagtt
     2701 tgaattaatc gggctcaggg ttgaagatgc cgaaggacaa cccttaggaa cactagaaga
     2761 agtgttggaa tatccccagc atcatgtgtt tgtgattcga aatgaggatc gtgaaattct
     2821 ggtccctgcc agtcggcgaa ccattgccat ggtagacctc ccccataagg ttttgcggtt
     2881 aacctcacgt gaatggtggg ataccacata tgcgttgtga cattttgaca ttgtttccgg
     2941 agttgattca ctctgtgagc tcacagagca tcatgaaacg cgcccaggat aagggcctgc
     3001 tcacactcaa tgtccacaac attcgagact atacccatga tccacatcgg acggcggatg
     3061 atacgccgta cggaggtggc ggcgggatgg tcatgaaggc cgaaccgatt tttcaagcca
     3121 ttgaccggat aacaccggac ctcgatgaaa tacgcatcat catcccctct ccacagggca
     3181 tcaggttttc ccatggactt gcgaatgacc tgagccagga tactcgcccg ttgttattta
     3241 tttgtgggca ctatgaggga attgatgaac gggtgcgaac acatttgcgt gttgaagaaa
     3301 tctcccttgg ggattatgtg ctgaccggtg gtgagttggc ggcactcgtg atgatcgatg
     3361 cggccatgcg tcttataccc ggagtattag gggatccaca ttctgctcaa cgagagtcct
     3421 ttgtagaatc cctattagac tttcctcatt tcacgaaacc cattgaaatt cgaggcatgt
     3481 cggttcccga cattttaatg tccggcaacc atgaag
//
LOCUS       DATIAL010000271         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1556084, whole genome shotgun sequence.
ACCESSION   DATIAL010000271 DATIAL010000000
VERSION     DATIAL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556084"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="VLA60_08330"
     CDS             complement(<1..1296)
                     /locus_tag="VLA60_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HSF09407.1"
                     /translation="MPTPPTTTSAARTLLGTSLLQDGWITQEQLDLAIRESKRKGVML
                     GQTLIGLGFITEKVLAHYLAEGTQTGLVDLSIPFIPPEILELVPYDLATQFQVLPIAK
                     QGNVLNLAMADPLNIVAVDTIERRTGLNVNTQTAPTQELIEAIERHYAHRESIKQLLD
                     ELMAKGISDLGEDGSREAPMIRLCNQLITAGIQSRVTDIHVEPDEYYLRVRMRIDGVL
                     TQEILIPKPLQAPVTARFKLMANLNLTEKRIPQDGRIPFTLGTRRIDLRVSTLPTNFG
                     ESLVLRILDKGALKLEFRALGFTLHDQSRVQSLIQRPYGIILVTGPTGSGKTTTLYTA
                     LRHVNAKEKSVFTLEDPIEYQMSLIRQTQVNPDIGMTFAAGLRALLRQDPDVILVGEI
                     RDQETADLAMRAALTGHLVFSTLHTNDALGAIPRLIDMGV"
     gene            complement(1430..2113)
                     /locus_tag="VLA60_08335"
     CDS             complement(1430..2113)
                     /locus_tag="VLA60_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSF09408.1"
                     /translation="MPRKLFRPMGHAQGFTLVEMIGVLAIIAILIALLLPKIFNLIAS
                     SNARSLAAAIRTYETAIVKYYADVGTVYPLNATGVPAANTSGDSATATSLPARLTLDV
                     SDPLNTGANQWIRFRGPYLEKFNSNVPPGLGTTMIMPATTAVALGTATTASNVGWDLK
                     GNDGNSDIPTGARVAYLRVDSISDTEFNEIDGIIDAGIGTTPAERQLRGRVKYDASND
                     RIYVYLVHQ"
     gene            complement(2266..>2980)
                     /locus_tag="VLA60_08340"
     CDS             complement(2266..>2980)
                     /locus_tag="VLA60_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09409.1"
                     /translation="WISWTTSILSALICMGTFTSSAALGNHLALPGSQDLHHYHNSAG
                     QPFIQQLETINKYTPPHSRSDLGTRASSIVEKPAVALPLLHPSIANEGKSGTLSRLPD
                     SVITKKAPTSAIPGQKVTAQSHVNIGWNLLLNGRLHAAMAAYREALRHSPHSAKAYIG
                     MGITLKSMGNIEGAKYAIQQAIELNPQLSAALVHWGYLHADGHFGPSDSETARRFFNQ
                     AFQLGDPFARIALLDLQDR"
BASE COUNT          704 a          695 c          807 g          774 t
ORIGIN      
        1 cactcccata tcgatcaacc ggggaatggc gcccagcgca tcgttggtgt ggagggtgga
       61 aaagaccaag tgcccggtca aggcggctcg catggccaaa tcggctgttt cctggtcccg
      121 gatttcaccc accaggatga cgtcagggtc ctgacgaaga agggcgcgta gaccagcggc
      181 aaatgtcata ccgatgtctg gattgacttg tgtctggcga atcagggaca tctgatattc
      241 aataggatct tccaatgtga atacactttt ttctttcgca ttgacgtgac gaagggctgt
      301 atacaacgtc gtagtctttc cacttcccgt cggaccggtg acgaggataa tgccataggg
      361 acgttggatc agagattgaa cgcgcgattg gtcgtgcagt gtgaatccca atgccctgaa
      421 ttctaacttt aaggccccct tatccagaat cctgagcaca aggctttctc caaagttcgt
      481 cggaagtgtg gacacccgaa gatcaatgcg gcgtgtccct aaggtgaacg ggatacggcc
      541 atcctgcggg attcgctttt ccgtaagatt gagattcgcc attaatttga accgggcggt
      601 aacgggagct tgaagaggtt tcggtatgag aatctcttga gtcaggactc catcgatacg
      661 catacgcacc cgcaagtaat actcatccgg ttccacatgg atatcagtca cccgggattg
      721 tattccggca gtaatgagtt gattgcacag acgaatcatt ggcgcttctc ggcttccgtc
      781 ttcaccgaga tcagagatac ctttggccat taattcatca agcaattgct tgatggattc
      841 cctatgagca tagtgccgtt cgatcgcctc gatgagttcc tgggtaggcg cggtttgagt
      901 gttcacattg aggcctgtac gacgttcaat agtatcgacc gccacgatat tcaagggatc
      961 cgccatagcc agattcaaga cattcccctg ttttgcaatt gggaggactt gaaattgtgt
     1021 ggcgaggtcg tatgggacga gctctaaaat ctctggtgga ataaagggaa tgctgaggtc
     1081 taccaggccg gtttgagtgc cctcggcgag gtaatgggca agaacttttt cggtgatgaa
     1141 cccgaggcca atcagggttt ggcccaacat gacccctttt ctcttgctct cgcgtatggc
     1201 gaggtctaat tgttcctggg taatccatcc gtcttgaagg agagaggttc ccagtaaagt
     1261 ccgtgcggcc gaggtggttg tgggtggtgt tggcatgggc cagcgtcctc aatcacctaa
     1321 aaagaactga gaattatgtt ccctgttatt ggggtggaga tgagaagttt gcgaaaggaa
     1381 ggtttcgtaa ataccaccat agaatgttca aaaggtctgc tttcgattat tattgatgga
     1441 cgagatacac atatattcga tcattagatg catcatattt gacacgccct cttaattgtc
     1501 gttccgcggg ggtggttcca atgccggcat cgatgatacc gtcgatttca ttaaattcgg
     1561 tgtcagaaat actatccact ctaagatagg ccaccctggc tcctgtgggg atatcactgt
     1621 ttccgtcatt ccctttcaaa tcccacccca catttgaagc cgtagtcgcg gttccgagtg
     1681 ccaccgctgt ggttgccggc atgatcatgg ttgttcccaa accgggagga acattggaat
     1741 taaacttttc cagatacggc cctcgaaacc gaatccattg attggccccg gtattcaacg
     1801 gatccgaaac atctagtgtt aacctggccg ggagcgaggt cgcggtggcg ctatctccgc
     1861 tggtgtttgc ggctggtacc ccggtcgcat tcagtggata tacagtaccc acatcggcat
     1921 aatatttcac aatggctgtt tcatacgttc gaattgccgc tgccagggac cgggcattgg
     1981 atgaagcaat gagattgaaa attttgggaa gtaacaaggc aatgagaatg gcgatgatgg
     2041 ccaatacccc gatcatttct accaaggtaa aaccctgtgc atgacccata ggccggaata
     2101 atttccttgg catattttca gatctccttt accaggcttg ctccgttgaa cgaggtttcc
     2161 ggctgaatat gggaaatcct ggataaaatt tccatgccaa agtccttcgg caccggcgac
     2221 ggcattcttg agccacgcct tctccatgaa tgaaaatttt caaggttaac ggtcttgcag
     2281 gtccagaagg gcaatcctgg cgaagggatc tcccagttgg aaggcttgat taaaaaatcg
     2341 gcgagctgtc tctgaatctg aaggcccaaa atggccgtct gcgtgcaggt atccccaatg
     2401 aacgagcgcg gcggaaagtt gaggattcag ttcaatggcc tgttgaatgg catatttggc
     2461 cccttctata ttgcccatac tttttaatgt gatacccatg cctatatagg ccttagccga
     2521 atgaggcgaa tgcctcagag cttcgcgata ggcggccatt gcggcgtgga ggcgcccgtt
     2581 cagcaataaa ttccatccga tgttcacatg gctttgggca gtcacctttt gaccaggaat
     2641 tgcagaggtt ggtgcttttt tggtgatgac actatcaggc aggcgggaga gagttcccga
     2701 tttgccttca ttcgctatgg agggatgaag gagcggtaag gccacagccg gtttttcaac
     2761 aatcgacgag gctctggttc ccaggtcgga ccttgaatgg ggcggtgtat acttgttgat
     2821 ggtttccagt tgttggatga agggttgacc tgcggagttg tgatagtgat gcaaatcttg
     2881 agatcccggc agcgccaaat ggtttcctaa ggcggcagac gaggtgaatg ttcccatgca
     2941 aatgagggca cttaggattg aggttgtcca ggatatccat
//
LOCUS       DATIAL010000272         7387 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1125173, whole genome shotgun sequence.
ACCESSION   DATIAL010000272 DATIAL010000000
VERSION     DATIAL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125173"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VLA60_08345"
     CDS             complement(<1..222)
                     /locus_tag="VLA60_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09410.1"
                     /translation="MPKNTETTDRHVVLGLFNPATSLPPVLDQIRSQGIPDILMEILS
                     PLPLETSLLNKPVRIPLHRLTITGGIVGIG"
     gene            complement(206..1534)
                     /gene="nrfD"
                     /locus_tag="VLA60_08350"
     CDS             complement(206..1534)
                     /gene="nrfD"
                     /locus_tag="VLA60_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HSF09411.1"
                     /translation="MTDRPTVWEEMPKINRDLLSPLQTTTWTYYLWVGVLSCFVAAGA
                     GAWTYQIQTGIGQTDLHHPIFWGSYIASFVFWIGVSHSGTFISGVLRLTNAEWRRPVT
                     RMAELMTVVALLVTALFVFYHLGRVWRFYYLIPYPNQRELWPNFRSPLMWDATAIFTY
                     ATASTIYIYLPLIPDFALARDRIQGWRKTVYRWLALGWHGSQAQWRTLEMAIRIITPL
                     IVMVMISVHSIVGFDFSMALVPAWHSTIFAPYFVVGAVHSGLGMVVIGLYTLRRVYRL
                     HDYVRTEHFEKLGKLLLVTTLVLAYMYFAEQLTVWYGKVPDHIAVQNSLLYGAYAVPF
                     WFMVSCIYVIPLVTLVWPRFRQWPGGLLMVGLIINIGMYIERVLIIVPPLAHPRLSFQ
                     WDEYFPSLIEFAILVGTLALALLLYSLAVKFVPVISIWEEKLGKKHAQEH"
     gene            complement(1531..2268)
                     /locus_tag="VLA60_08355"
     CDS             complement(1531..2268)
                     /locus_tag="VLA60_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSF09412.1"
                     /translation="MSQDPQATEEYSKTAASPYKWEMVVDLDRCSGCEACVVACHAEN
                     NIRISGEEEAAEGRAINWIRIERYWEGDYPNVKAKFLPVMCQHCDDAPCEPVCPVYAS
                     YHTPEGLNAQVYNRCIGVRYCGNNCPYVARQFNWFDPHWDEPLAQQLNPDVSVRSGGV
                     MEKCTFCVQRIRSGKEAAHKEGRRVRDGEITPACVQACPTSALIFGDRNDPDSRVSHL
                     KKSRRGFHLMESLGTHPAVTYLRRLSE"
     gene            complement(2268..4463)
                     /locus_tag="VLA60_08360"
     CDS             complement(2268..4463)
                     /locus_tag="VLA60_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF09413.1"
                     /translation="MDLNRRTLLKILGLTAAGSSLPGCEREVHNLVPYLLPDDHIVPG
                     VANWYASTCQECEAGCGTIVRVMEGRAKKIEGNPKHPVNEGKLCARGQAALQGLYNPD
                     RLQGPLRREGQRGQGQFRPITWEEGLAEWVEQLRTHAGKSAMVTRPVSGTLGSLLTTL
                     MDSISGRLVMYESTGEQTLRTANHLSFGTHDLPYYDLANADYLLSFGAPFLEHWLSPV
                     SFGVAYGKFRQGRPLVRGRFIQVEPRLSLTASNADQWIPLRPGTEGFLAMGIGQILLR
                     EGLTGLSSSHLASFHQTFSAFSLEDISAKTEVSEEIITQLAHELTSANAPLILGGGPA
                     AAQTNGTTTLILINALNVMIGAIGRRGGLQWMEPQISPVDLARPGLASERTLMDLARE
                     FEQGSRTLLHLYRADPLFTLPPSLKFERVFDHAQFIVSFSSFLDDSTSMADLILPDHD
                     PLESWGDVVQQDTIPVLTWSVSQPVVGVLYDTRPIGDVWLDAAHRLGGPLREQVPWNT
                     LHDMLKSRWQTVLTKHDSPQSFDTRWREALQEGGLWPSNIPMRQMNPVSPSVTYEPPQ
                     FLGNSSDYPLYCYPYPSLGLHDGRGANRPWLQELPDPLTTGMWGSWIELNPTTAQALG
                     IHSGDRVRVTSDYGSIESSAMVFPGLHPDIIAVPMGQGHRAYGRYAKGRGVNPMALLG
                     PSFDGQSGSLATGGTRVRVERIKGGSRLPMLEQSGKNPLFGRIQETGGL"
     gene            complement(4476..4994)
                     /locus_tag="VLA60_08365"
     CDS             complement(4476..4994)
                     /locus_tag="VLA60_08365"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HSF09414.1"
                     /translation="MKSGRIKGVILALLAGSVATGIGALYSIGSADLPVQDQQPVRFS
                     HRQHAGTLNIHCQFCHRYARQSPVAGVPSMALCQTCHESLSETTPGMKMLGIYWKEQR
                     PIPWVRLQRLPDHVYFTHEMHLFAGLSCTSCHGNVARMSSTPRAPTYEMGWCLTCHEQ
                     RRASHDCWTCHK"
     gene            complement(5079..5522)
                     /locus_tag="VLA60_08370"
     CDS             complement(5079..5522)
                     /locus_tag="VLA60_08370"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSF09415.1"
                     /translation="MPPPFLMMFFGFILFLAPFSQSVAITPNQGSYKASELVGKSVKN
                     LQGEDIGQIEELVIGSNGEVGYAVLSFGGFLGVGDKLFAVPWTSLAHQPDREYLTLDI
                     QPEKLEKAPGFNKNDWPDMKDERWKFLIWEFYSTPDHIPQNFKTR"
     gene            5815..6222
                     /locus_tag="VLA60_08375"
     CDS             5815..6222
                     /locus_tag="VLA60_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09416.1"
                     /translation="MIFLVTENQKFLELMTGRLRKMGKSAVGFSHSQDLWVRLRQTSP
                     DIIILDGNCPNIDAIEILKNLKAAGYKGQTIVLGDESSTPFVPEVSRFGAIQTAGRPL
                     AVNRVVGAIRIAQEHLNQDVCYSSASTQEVSWL"
     gene            6315..6704
                     /locus_tag="VLA60_08380"
     CDS             6315..6704
                     /locus_tag="VLA60_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09417.1"
                     /translation="MPNTNASFSAFSRVDQLKHQLKKLEDLSNSQVNTEVLDKFDRET
                     EEILKRVDGREDKMAAYNFATMAEAEALVNLPESAQEPTSRDLLQKSLQQRRQVLLGL
                     VSEMESLEETEAEVLTGEDKEDPPLMG"
     gene            complement(7035..>7387)
                     /locus_tag="VLA60_08385"
     CDS             complement(7035..>7387)
                     /locus_tag="VLA60_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSF09418.1"
                     /translation="NHTIPPALSNVLDHFLEEYFRKPSAIVCYSAGGFGGVRAAMTLR
                     AMLAELGMSSIPSLFPISHVQDAFQEDGTPTDQNFHQKALEFFEELEWYAYALKQART
                     HPCPRSESSTQSLT"
BASE COUNT         1921 a         1884 c         1862 g         1720 t
ORIGIN      
        1 tccaattccc acgatccctc cggtaatggt caggcgatgg agcgggatgc gaaccggttt
       61 attgagcaaa gaggtttcca gcggaagagg ggagaggatt tccataagga tatcgggaat
      121 gccctggctt cgtatttgat caaggacagg agggagagaa gtcgccggat tgaataatcc
      181 cagcaccacg tgcctgtctg tcgtctcagt gttcttgggc atgttttttc cccagttttt
      241 cttcccagat cgaaatcacc gggacgaatt tcacggcaag cgaatacagt aagagagcca
      301 aggccagcgt tcctaccaga atggcgaact ctatcagcga agggaaatac tcatcccact
      361 gaaatgacag cctcggatgg gcaaggggag ggacaatgat cagcacgcgc tcgatataca
      421 tgccgatatt gatgatcagc cctaccatca ggagtcctcc cggccattgt cgaaaacgag
      481 gccataccag cgtcaccagg ggaatgacat aaatacaact caccatgaac cagaagggca
      541 cggcatacgc tccatagagg agagaattct gcacggcaat atgatcgggc actttgccat
      601 accagacagt caactgctca gcaaaataca tataggccag gaccagggtg gtgaccagca
      661 acaatttccc gagtttttca aaatgttcgg ttcgaacata atcatgcaga cggtaaaccc
      721 tgcgcagggt atacagccca atcaccacca tccccaagcc tgagtgcacc gcgccgacca
      781 caaaataggg agcgaaaata gtcgaatgcc atgccggcac aagagccatg ctgaaatcaa
      841 atccgacaat ggaatgcacc gagatcatga ccattacgat caggggggtg ataatgcgta
      901 tggccatttc caatgtgcgc cactgcgctt gagatccatg ccaccctaac gccagccaac
      961 gatacacggt ttttctccat ccttgaatcc gatcccgggc cagcgcaaaa tcaggaatta
     1021 agggcagata aatatagatg gtgcttgcgg tcgcatacgt aaaaatggct gtagcatccc
     1081 acatcagcgg agatcgaaag ttcggccaga gttcccgttg attggggtat ggaatcaggt
     1141 aataaaaccg ccacacccgg cccaggtgat agaagacgaa taacgcggtg acgagaaggg
     1201 ccacaacggt catgagctca gccatacgcg tgacaggtcg gcgccattcg gcattcgtta
     1261 accgcaagac accggaaata aacgttccgg aatggctgac gccgatccag aagacgaacg
     1321 aggcaatata ggatccccag aaaatgggat gatgcaaatc ggtttgtccg atacccgttt
     1381 gaatttggta cgtccacgct cccgcgcctg ctgcgacaaa gcaagacaac acgcctaccc
     1441 agagataata ggtccaggtg gtcgtctgga gcggcgagag aagatcacga ttgatcttgg
     1501 gcatttcctc ccaaaccgtt ggccgatcgg tcattccgac agcctccgca aatacgtcac
     1561 ggccggatgc gtacccagag attccatgag atgaaaccct ctccggctct tcttcaggtg
     1621 agacacccgg ctgtcgggat cattccgatc accaaaaatc agggcggatg tgggacaggc
     1681 ctgcacacag gcgggtgtga tttctccatc tctgacccgc cgaccttctt tgtgggctgc
     1741 ttcctttcca ctacgaattc gttgcacgca aaatgtgcat ttttccatca caccgccgga
     1801 tcggacggac acatccggat ttaactgctg tgccaacggc tcgtcccaat ggggatcgaa
     1861 ccaattaaat tgacgggcga catagggaca gttattcccg cagtatcgaa caccgataca
     1921 acgattatag acctgggcat tcaacccctc cggggtgtga tacgaagcat agacgggaca
     1981 gacgggttca cagggagcat cgtcacaatg ctggcacatg accggaagga atttggcttt
     2041 gacgttggga tagtctcctt cccaatatcg ctcaatgcga atccaattga tcgctcgccc
     2101 ttcggcggct tcctcctccc cggagatgcg aatattgttt tcggcatgac acgccactac
     2161 acaggcttca catccggagc accggtccag atcgacgacc atttcccatt tatagggtga
     2221 tgcggccgtt tttgaatact cttccgttgc ttgaggatct tgactcatta caaacctccc
     2281 gtttcctgta tacgcccgaa gagagggttc tttccggatt gttccaacat cggcaaccgc
     2341 gatccgcctt tgatacgctc aacacgaact cgcgtccctc ctgtggccag agaacccgat
     2401 tgaccatcaa aacttggacc aagcaaggcc atgggattta ctccgcgccc cttggcatat
     2461 cgtccgtacg cacggtgccc ctggcccatg ggaacggcaa taatgtcggg atgcaggcct
     2521 ggaaacacca tggctgacga ttcgatcgac ccataatccg acgtgacacg aacccggtcg
     2581 ccagaatgta ttcctaacgc ttgggccgtg gttggattga gttcaatcca acttccccac
     2641 atgccggtgg tcagaggatc cggcaattct tgaagccagg gacggttggc cccacgtcca
     2701 tcatgcaacc ccagggacgg atagggataa caatacagag ggtagtcgga ggagtttccc
     2761 agaaattggg gcggttcata ggtgacggag ggtgagaccg ggttcatctg acgcatgggg
     2821 atgttggaag gccacaatcc gccttcttgc aaagcctctc tccagcgggt atcaaatgac
     2881 tgtggcgaat catgcttcgt taggacagtc tgccaccggc tcttcagcat atcgtgaagg
     2941 gtattccagg ggacctgttc acggagaggt ccgcccaatc gatgagcggc gtccaaccac
     3001 acatcaccga tgggcctagt gtcatagaga acacccacca caggttgcga tacactccag
     3061 gtcagcacgg ggatcgtatc ctgttgaaca acatcccccc acgattccag cgggtcatga
     3121 tccggcagaa tcaaatcagc cattgaggtc gagtcatcaa ggaaggaact gaaacttacg
     3181 atgaactgcg catggtcaaa cacccgctca aatttcagtg acggtggaag ggtgaacaga
     3241 ggatcggcgc gatacaaatg aagcagcgtg cgggatcctt gttcaaattc acgggccaaa
     3301 tccataagcg tccgttcact ggccagcccg ggtctggcca gatccaccgg tgagatttga
     3361 ggttccatcc attggagtcc gccgcgccgc ccaatagcgc cgatcatcac gttgagtgca
     3421 ttaatgagga taagagttgt tgtgccgttg gtctgggccg cagccggacc cccacctagg
     3481 attagcggag cattcgctga tgtcagttca tgcgccagtt gggtgattat ttcttcggag
     3541 acttccgttt tggccgaaat atcttccaat gagaaagcag aaaaggtttg gtgaaaggat
     3601 gccaggtgag aagaagaaag ccctgtcagt ccttcccgta ataagatctg tccaataccc
     3661 atcgccagaa acccttcggt tcccggccta agaggaatcc attgatccgc attcgacgcg
     3721 gtgagggaca ggcgcggctc cacctgaata aaacgacctc gaaccaacgg gcggccttga
     3781 cgaaattttc cataggccac accaaaggac accggtgaga gccagtgttc aaggaatggc
     3841 gcaccgaatg acaagagata atccgcattt gccaggtcat aatatggaag gtcatgggtt
     3901 ccaaagctga gatggttggc tgttctgaga gtctgttccc ctgtggattc gtacatcacg
     3961 agacgaccgg agatggaatc catcaatgtt gttaacaggc tgcctaatgt gcctgacacc
     4021 ggacgggtaa ccatggccga ctttccggca tgcgttcgta gttgctccac ccattctgcc
     4081 aggccttctt cccaggtaat cggccggaat tggccttgac ctctttgacc ttcacgtctt
     4141 agaggacctt ggaggcggtc gggattgtaa agaccctgaa gagcggcttg tcctcgggca
     4201 cagagttttc cctcattaac cggatgcttg ggattgcctt caattttttt tgcccgccct
     4261 tccatgacac gaactatggt cccgcaaccg gcttcgcatt cctgacatgt ggaggcatac
     4321 caattggcta ctccgggaac aatatggtcg tccggcaaca gataaggcac gaggttatgc
     4381 acctctcgct cacatcctgg cagggacgac ccggctgctg tcagtccaag aatcttcaat
     4441 agggtccggc gatttaggtc catggccgtt tccttttact tgtgacaggt ccaacaatcg
     4501 tgagaggccc gccgttgttc atggcaggtc aaacaccagc ccatttcata agtgggcgct
     4561 ctgggggtgc tcgacatacg ggcaacattt ccatggcatg aggtgcacga caggcccgca
     4621 aacagatgca tttcatgcgt gaagtagaca tgatccggaa gacgctgcag tcggacccat
     4681 gggatcggcc gttgttcttt ccaatagatc cctagcattt tcatacccgg tgtcgtttca
     4741 gaaagggact catggcacgt ctggcacaag gccatggacg gcacaccggc cactggagat
     4801 tgcctggcat atcgatgaca aaattgacaa tggatgttca atgttcccgc atgttgtcga
     4861 tgactaaacc gtacaggttg ctgatcttga acgggtaggt cggcacttcc gatggaatac
     4921 agtgcaccga tccccgtggc aacacttccg gctaacaacg ccaagatgac tcccttaatt
     4981 cttcctgact tcattcttat ccagatgtga gtatggtgag tgaattattg ctgatacccc
     5041 ttcattcttt tcctcttggt cccattgccc acccaggctt accgggtttt gaaattctga
     5101 gggatgtgat ccggcgttga gtaaaattcc catatgagga atttccacct ttcatccttc
     5161 atgtccggcc aatcattttt attaaagcct ggtgcctttt ccaatttttc cggctgaata
     5221 tccagagtca aatactcgcg atccggttgg tgagccagcg aggtccaggg cacagcaaag
     5281 agtttatctc caacacccaa aaatcctccg aaggataaaa cggcatatcc gacttctcca
     5341 ttgcttccaa taaccaattc ctcaatctgc ccaatatctt cgccttgcaa attcttgacg
     5401 gattttccaa ccagctcact ggctttgtag gagccctggt taggagtaat ggctacggat
     5461 tgagaaaagg gtgccaggaa taggatgaag ccgaaaaaca tcattagaaa gggtggtggc
     5521 atatagatcc ccatactgcc gttgattcat atggccttcc tactaaaatt cacttacgga
     5581 aagagctgat ttcttcaatg tagttctcta tctcactctc taaaatagac aaaataggat
     5641 cgttcatcag cactttgtct accgtcttct aaaggccctt acacaggcag atggactatg
     5701 aaggaattac taaatttgca ccttcaaggg tttttgatag tggcggaaac cgtcgaagtt
     5761 cgctaaaaag cgccaggctg tttctgtcaa catgaatgca tgaaggaggc gcagatgatt
     5821 ttccttgtga cagagaatca aaaattttta gaattgatga caggccgatt gcgaaaaatg
     5881 ggaaaaagtg cggtgggatt ttcacatagt caagatttgt gggtgcgcct ccggcaaacc
     5941 tcccctgata tcatcatcct cgacggtaat tgccctaata ttgacgcgat agaaattctc
     6001 aagaatctta aggccgccgg gtataaaggc caaaccattg tgctaggaga tgaatcttct
     6061 acccccttcg tccctgaggt ttctcgattt ggtgcgattc aaaccgccgg tcgacctctg
     6121 gccgtgaatc gggtagtggg cgctattcga attgcccagg agcatttgaa tcaggatgtg
     6181 tgttattcct cggcgagcac acaggaagtg tcctggttat gaggtcgaaa tttttacaca
     6241 caggacatga gatggtagag acgaaaaatt attcaaagta gaaaatcaga cctaaatgaa
     6301 gggggtatgt tcttatgcca aacaccaatg catcattttc tgccttttcc cgggtagatc
     6361 aactgaaaca tcaattgaaa aagttggagg acctgagtaa ctcccaggtc aataccgaag
     6421 tgttggacaa gtttgatcgt gagactgagg aaatattaaa acgggtagac ggcagagaag
     6481 acaaaatggc tgcctataat tttgccacca tggcggaagc cgaagccttg gtcaatcttc
     6541 ctgagtctgc tcaagaaccg acttcccgtg accttttaca aaagtctttg cagcaaagac
     6601 gacaggtgtt attgggattg gtcagtgaaa tggaaagcct ggaagaaaca gaggcggaag
     6661 ttcttacggg agaagataag gaagatccgc cgctaatggg ttaatcccag tattcatgtt
     6721 aaaaaagttt atcttaggaa cgttatccct gtctgttttt actgtgggat tattcttgaa
     6781 ttatttggcc aacttcaaag aacctcattt ccgatgccaa tagaacccaa ctctgccctg
     6841 tcatcatgag tggcagagcc atatccgctt cacccaaaca ataatacggc tccattaagt
     6901 cgctattaga ccgcgcagga cagaaggctt tcaagcatca aagatgtttg cacgtcagtc
     6961 ttgtgctgag tggtctctca gtctcttctc tcaagcagtt catgaaatgc catgttcgat
     7021 caagcctggc acactcaggt aagcgattgt gttgaacttt cacttcgagg acaaggatgg
     7081 gtcctggcct gtttcagggc atacgcatac cattccagtt cttcaaaaaa ctctagggcc
     7141 ttttgatgga aattttgatc agtaggcgtg ccatcttcct ggaaggcgtc ctgtacatgg
     7201 gagatgggga acaaagatgg aatactggac attcccagtt cagctaacat ggccctgagg
     7261 gtcattgccg cacgaactcc gccaaaccca cctgccgaat aacatacgat ggcggaaggt
     7321 tttcgaaagt attcctcaag gaaatggtct aatacgttcg aaagggcagg aggaatggta
     7381 tgattat
//
LOCUS       DATIAL010000273         2754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_480244, whole genome shotgun sequence.
ACCESSION   DATIAL010000273 DATIAL010000000
VERSION     DATIAL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480244"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VLA60_08390"
     CDS             complement(<1..813)
                     /locus_tag="VLA60_08390"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09419.1"
                     /translation="MSAFKPHAASEGDPPVASLPDPTPLLLASGLPSQDVAHILEAYS
                     LQDWKRADQNLQAMAGEPVTRKALATILPAVLSSVGRTADPDQAINEWERYVDSGIQR
                     LQLFQYLAMVPHVVEVLGAAFGNSPAMAQTFIRDPLLVYWIEDDGVLRRRMTRAALEA
                     AVQAALSTVTSFEAKCEALRRLKRREMLRIGIRDLLGIATPVETYTVLSDLASAVIQA
                     VYELVNRELTQRFGDFGEGTATGKKVDGFSVLAMGKLGGWELNYSSDVDLIYV"
     gene            855..>2754
                     /locus_tag="VLA60_08395"
     CDS             855..>2754
                     /locus_tag="VLA60_08395"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09420.1"
                     /translation="MQQFFSILGTMNEAERIQSYQTQCPDLRPALIRDFVRQMDPDYF
                     DSFPPAAILEHLALANQLTFERPCAISIRTLPTRQYQLTLVGYDYFSEFAMFCGVLSS
                     FGLDIREATIFTSLETTSPASLSTKSSPGHLPMGGSSHGTRGLTRKIVVDVFHVQALE
                     DVKFEEPEQRELQEMVTALLILLQKNQIRQARRQVNRRLIENLENMRQKPTEMVHPVH
                     ISFSNPRGSHETILDISSTDTPAFLYTFANALAMRGIYIVKAKIEVAKHRVRNRLYVR
                     GRQGEKIQGKAEQQELRTAATLLKEFTHYLRWAPDPGKALDHFDQFLDQWLEQANTPS
                     HLTKLSRASTLERLAQLFGSSDYLWEDLLRRQHNNLLPMMNQYQKGPLIRSKAILSKA
                     IEPLLLKAKTPADKKQRLNQWKDEELFRIDMRHLLENSPLPQFSQALTNLADVILNQA
                     LLHSQQAINPKASLTAQPSMAIFGLGKLGGGELGYASDIEILFVYHMPGKPSRGQTAQ
                     GSSDYFERWAQEFLQWIEAKQEGIFHLDTRLRPHGEKGLLANSLEEIQRYYTSEGGAA
                     PFERQALIKLRFLAGNRAVGKAVEQHRDQFVYAPDPWDLQTALHLRERQIKELVRPGT
                     THVKYGAGG"
BASE COUNT          656 a          802 c          689 g          607 t
ORIGIN      
        1 cacatagatc agatcgacat ccgaactata attaagctcc catcctccga gtttccccat
       61 ggccagcacg gagaatccgt cgaccttttt cccggtcgct gtcccctctc caaaatcgcc
      121 aaaccgctgc gttaactccc ggttcaccag ttcatagacc gcctgaatca cggctgaggc
      181 cagatccgat aacaccgtat aggtttcgac cggcgtggcg atgcccaata agtcccgaat
      241 cccgatccgg agcatttcgc gccgcttcag ccgtcgtaac gcttcacatt tcgcttcaaa
      301 actcgtcacc gtactgaggg cagcctgcac ggcggcctca agagccgccc gcgtcatccg
      361 ccgtcgcaga actccgtcat cttcaatcca atacacgagt aacggatcac gaataaaggt
      421 ttgtgccatg gccggactat tcccgaacgc cgcgcccaac acttcaacca catgcggaac
      481 catcgctaaa tattgaaaca gttggagccg ttgaataccg gaatcgacat accgttccca
      541 ttcattgata gcctgatccg gatcggccgt tcgccccacg ctgcttaaga ccgccggcag
      601 aatggtggcc agggcttttc gggtgaccgg ttctccggcc atggcctgca gattctggtc
      661 ggcccgtttc caatcctgaa ggctataggc ctcaagaatg tgggccacgt cttgggaggg
      721 aagtcctgaa gccagcagta agggagtcgg atcgggaagg gaggcaacgg ggggatcacc
      781 ctcagaggct gcgtgtggtt tgaaagcgct catcgtcaaa gggaaaatta tacgggagcg
      841 cggcagacct tacaatgcaa caatttttca gtatactcgg caccatgaac gaagctgagc
      901 ggatccagtc gtatcaaacc caatgtcctg atcttcgtcc ggccttgatc agagattttg
      961 tccggcagat ggacccggat tattttgaca gctttcctcc cgccgctatc ctggaacatt
     1021 tagctctggc aaaccagctt acgtttgaac gaccctgtgc gatctcgatc cggactctcc
     1081 ccacccggca atatcaactg accctggtgg ggtacgacta tttttcagaa tttgccatgt
     1141 tctgtggcgt cttatcgtcc ttcgggttgg acattcgtga agccacgatt ttcacttcac
     1201 tggagaccac atcccccgct tccctttcca cgaaatcatc tccgggacac ctccccatgg
     1261 gtggatcttc acacgggact cgggggttga cccgaaaaat tgtggtggac gtctttcacg
     1321 tgcaggctct tgaagacgtg aaatttgagg aacccgaaca acgtgagctt caagagatgg
     1381 tgacggccct cctcatcctt cttcaaaaaa atcagatccg gcaagcccgt cgccaggtca
     1441 atcgccggct cattgagaat ttagagaaca tgcggcagaa accgacggag atggtccatc
     1501 cggtgcacat cagtttttct aacccgcgcg gcagtcatga aactatcctg gatattagct
     1561 caaccgatac gccggcgttt ttatacacct ttgccaatgc cctggccatg cgcggcatct
     1621 atattgtgaa agccaagatt gaagtcgcca agcatcgcgt ccgcaaccgt ctctacgtcc
     1681 gcgggcggca aggcgaaaaa attcagggga aagccgagca acaggaactg cgaacggcgg
     1741 ccaccttact gaaagaattt acccattatc tgaggtgggc accggacccc gggaaagcgc
     1801 tcgaccattt tgatcaattc ctggatcaat ggttagagca ggcgaacact ccatcgcatc
     1861 tcaccaagct gagccgggct tccaccctgg aacgactggc gcaactcttt ggcagcagtg
     1921 attacctgtg ggaggactta cttcgccggc agcacaacaa tttgttgccc atgatgaatc
     1981 agtaccaaaa aggtcccctg atccggtcaa aagctatact cagcaaagcc atcgagccgt
     2041 tattgctcaa agccaaaact cccgccgaca aaaagcagcg tttgaatcaa tggaaagatg
     2101 aagaattgtt tcgcatcgac atgcgtcatc tcctggagaa tagtccgctc ccgcagtttt
     2161 cccaagccct gaccaatctt gctgatgtca ttctcaatca ggcccttttg cattctcaac
     2221 aggcgatcaa tccaaaagcc tccctcaccg ctcagccgtc gatggcgatc tttggattag
     2281 ggaaacttgg agggggcgaa ttagggtatg cctccgatat cgagattctg ttcgtctatc
     2341 acatgccggg caagccatcg cgcggtcaga ccgcgcaggg atcctcggat tattttgaac
     2401 gctgggcgca ggaatttctc caatggattg aagccaagca ggaaggcatt ttccacctgg
     2461 atacccggct tcgaccgcat ggcgagaagg ggctcctcgc taattccctc gaagaaattc
     2521 agcggtatta tacgtcagag ggcggagccg ccccctttga acgacaagcc cttatcaagc
     2581 tccggtttct tgccggcaat cgtgcggtcg gaaaagccgt tgagcagcat cgtgaccaat
     2641 ttgtctatgc ccccgaccca tgggatttgc agaccgccct ccaccttcgt gaacgtcaga
     2701 tcaaggaatt ggtccggccg ggaaccacgc atgtgaaata cggggcgggt ggcc
//
LOCUS       DATIAL010000274        13628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1556871, whole genome shotgun sequence.
ACCESSION   DATIAL010000274 DATIAL010000000
VERSION     DATIAL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13628
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556871"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VLA60_08400"
     CDS             complement(<1..965)
                     /locus_tag="VLA60_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF09421.1"
                     /translation="MKKLLWIGVVVIVSVIAARQFWLTGVRSAPIQYRTAVVERGPIV
                     AIVGATGTINPVTSVQVGAQVTGKIISLHADFNSVVKAGEVIARIDPSLFQTRRDQAA
                     ANLVNAKAMWSKMRTELAQRKRELDRTQTLFQRELVSQNDLDMATTAYESAQAQLEVS
                     GAQIKQAQALLDTADLDLKYTVIRSPVNGIVIARNVEVGQTVTAGFTTPNIFLIALDL
                     TKMQVDTNVSESDIGGIREGQEALFTVDTYPQVDFRGVVRQIRNAPIIVQNVVTYDVV
                     VEVDNRDLRLKPGMTANVSITVARKDDVLMIPNAAFRFFPSQSASV"
     gene            complement(1861..2319)
                     /locus_tag="VLA60_08405"
     CDS             complement(1861..2319)
                     /locus_tag="VLA60_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HSF09422.1"
                     /translation="MLVRISYLFGHLVFFGFCLLGVAGSWGQGVPGQSVEESGGFVIP
                     ESIKGFDEQKVKNDPICDSSVRPQIDHVKPDEMKTGDSVVVEGKNFGKKKECLHGVTF
                     GSVPAKAFSFVDDERVEVVVPEGLRSGVTFLNVETGGGTARKGILIRSKD"
     gene            complement(3070..3387)
                     /locus_tag="VLA60_08410"
     CDS             complement(3070..3387)
                     /locus_tag="VLA60_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09423.1"
                     /translation="MDIDTFRQMVAKKPDGFLGRYGLGDKLLKEGEHLEEAVEHLRVA
                     VALDPIHVASHLALGRALAGLKRYEEAKTVLAAGIDAALSGRSSGGGDLVPEMRALIQ
                     TLG"
     gene            complement(4017..4916)
                     /locus_tag="VLA60_08415"
     CDS             complement(4017..4916)
                     /locus_tag="VLA60_08415"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09424.1"
                     /translation="MKNGKLNISPGIPTPDISRIQESFEIVASHGEQIVFRFYNVLFD
                     KFPEFQTFFSQAQLAQQYAAFLSGFRTLVLHMENPEELRSSLVQLGERHRKYGIKSKH
                     YPPVVYALLHVLMELGGESMDGKTYDAWENFLHLMRAIMLEPYSLEMLPDGNRQEKFL
                     STGAGGNTKRILLIDDDRQLLDLYQSYLELEGYLCSQVSDVAWAFTHIQMSHYDLVLT
                     DFQMPAMNGIQLRRNLEYVGNGCCPPFVLMTGSPNQEIRRQALESGFEAVLKKPHDLP
                     ELSSLVGKVLKKSTGFQGNLQDL"
     gene            complement(4962..5327)
                     /locus_tag="VLA60_08420"
     CDS             complement(4962..5327)
                     /locus_tag="VLA60_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09425.1"
                     /translation="MKSQKRILIVDDDADVLLFLRDRLNALGFEVLTAANGKEGVETL
                     QHHTVNGVLLDLCMPVMGGLLMLEQLAQSSTLPPVIVMSPSEHRSEMQLAIIKGAMDY
                     LIKPIAPDILTDKCLRLFY"
     gene            complement(5427..5876)
                     /locus_tag="VLA60_08425"
     CDS             complement(5427..5876)
                     /locus_tag="VLA60_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09426.1"
                     /translation="MKHLNWATASPTLTVLGNFLLFLSICGPASASTQPSDVWVSMDY
                     WAPYYRPALAIVPDGVSIHVVNPTSSPHSVTHDGCRKGGPCAFDTGGVQPGQEFTIPS
                     LPPGRYPYYCVLHPIMRGEIIVLPHHTVIGQGAIHDHLTITDVKDSQ"
     gene            complement(6311..7024)
                     /locus_tag="VLA60_08430"
     CDS             complement(6311..7024)
                     /locus_tag="VLA60_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF09427.1"
                     /translation="MALGSRILAVDDERQIRRSLQINLEAKGYEVLTAETGEEALQIM
                     SHRLPDLVLVDLLLPGMDGIDLTRQIRETYQIPIIILSAIGEEAKKIEALEVGADDYV
                     TKPFSMEELTARIRSVLRRTSSIYGTDPVFTFGNLQVDFESRNVCIRNQAVKLTPTEY
                     DLLKYLIQNSGKVLTHGTILRAIWGAGYSEQAQYLRVFIGNLRKKLEKNTARPQYILT
                     DPGVGYRFATDFEEDETPP"
     gene            complement(7036..9009)
                     /locus_tag="VLA60_08435"
     CDS             complement(7036..9009)
                     /locus_tag="VLA60_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09428.1"
                     /translation="MKPPKRSWLGTGSIGRALLILSTVFILALFGILLYTITTIQNQK
                     LDSVMVDLAGQQRMLSQRLMNEILLISQGIPAEYRFTQKMLKQHLDALLVGGKAVINQ
                     ESGDTAVLPPPPSQDIRQALGEQQILMAEFMQRADTFLKTRSDHPGYSLELDGLLALN
                     ARLIEVANKAVKLYSRNSQEKISNMIVWESLIGTVVIIFGILITRQVKLANQELEHEI
                     QERSRIETALRYRIEIENLVTDISTQFISLETKDLDAGINRALEAIGTFGGVDRSYVF
                     IFEDDGKTINNTHEWCQTGIEPQLSKLQGLRMQETPWFAERLISGPFFQISSVANLPL
                     EASAEKQLFQRQQIQSLVIVPMVLRGKLFGLLGFDSVRHEKTWSKEDIRLLQMAGEIF
                     VNGFERKEVEENLRKSEAAKVEAFRQSDALKTALLSLVSHELRTPLTAIKASIAGLLE
                     LSRQDASNVQQEFLQGINQEIDFLNGLVDNLLDMSKVEAGTLVPHREWHLLEDLVEGA
                     IRRLEMPLKSRSLQVNLGEKISPIFVDGMEIQQVLINLLDNAIKYSPPGSLVSIVGRM
                     TPNQVEVRVSSEGEGIPREELVSIFNRFYRLKGPRTRLIRGTGLGLAICKGMVEAHGG
                     RIWAESQNRIVTISFTLPIPDAPPMINFDREEE"
     gene            9364..10488
                     /locus_tag="VLA60_08440"
     CDS             9364..10488
                     /locus_tag="VLA60_08440"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HSF09429.1"
                     /translation="MNKQSQMHLDRQKVSDPLIVLEGSAPQSGKSPLPSDLRKTGLHP
                     DNWYPLARSKDLKRGKMLGVSFAGNPIVLVRTENEMVYALEDRCAHRQVPLHCGVVHG
                     EYVQCGYHSWTFDRTGRCMGVPYLEKNQPRPAGVRSFPCREAYGLVFVFPGAVSRVNE
                     IRFPDVSLANDPQYKTRYLDRQVGCHYSFMHENLMDMNHQFLHRRLMGGIRTVFLELR
                     ERPHAVEVDYTFSRIKGAQPLGEKLILGRTKASADGPKHDLMTICTNYPYQTLQFWTA
                     GSIHPALNLWNVYVPVDPAQRINHTFGLMMVRKPSIPGLIHLLWPFIVWFTNGIFAQD
                     RWIVEEEQKAFDRQGADWNQEVFPVIQSLRSLLGREGIAL"
     gene            complement(10578..11606)
                     /locus_tag="VLA60_08445"
     CDS             complement(10578..11606)
                     /locus_tag="VLA60_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09430.1"
                     /translation="MNHLPSSPPLAKDLMDPKALPLRSGKFENDLALQFLSGQYCGWP
                     VVDSTRKILGIVSDLRLFEAVSRLNSLDELRVEDTMTPPVYVHESESLDMVLNLMIQT
                     QIQRMPVVGDQNLVGVISKANVLRHCLPPSTSSQFVSSCAWCERVPDPSVAPASAKGM
                     RTLASFLSMEHLKFSEIDLVHTYCPSCLQTLQALNTASGSLSSDETETPEVRPRLLVV
                     DDDPSVGSLLRQALTEWGYKVLVARNGWEGLAVASRQTVDGILLDMDMPIMDGRTMLD
                     ELRWLGHQMPVMMMSGASDERALRQLLQEGAQGFFVKPFHLKSLKEACRRILQKNEAP
                     SSFRYHVV"
     gene            12426..>13628
                     /locus_tag="VLA60_08450"
     CDS             12426..>13628
                     /locus_tag="VLA60_08450"
                     /inference="COORDINATES: protein motif:HMM:NF040546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HSF09431.1"
                     /translation="MMHITEPTEKRLLLRHHQGAILFVSCYELGHQPLGIAQPMGFLQ
                     QAGFSPVGLDIAVDTIDEPALRLARFVGISVPMHTALRLGVRVAELIRAINPACHICF
                     YGLYASINAEYLLQELADTVVGGEYEIPLRSLIEALDRGQDANHHEGVSSSDRVVVPY
                     LKRPYSTTSTTSQPIPSRTGLPMLDQYARLEENGTERLVGYVETSRGCLHLCLHCPIV
                     PVYEGRFFLVPEAVVLEDIRNQVHAGATHITFGDPDFLNGPGHSLSIVRRMHEEFPHL
                     TFDFTAKIEHLLKHRPLMPDLEAQGCLFIISAVESFSNHVLEQLHKGHTGADIITAFT
                     ILREAGITLRPSLVAFTPWTSLDDYLRMLDMVEDHDLIDATDPIQLSIRLLVPPGSAL
                     LTKSDIQRF"
BASE COUNT         3641 a         3549 c         3056 g         3382 t
ORIGIN      
        1 acagacgcgg attgggaagg aaaaaaccgg aaggccgcat tcggtatcat taagacatcg
       61 tccttccgcg cgaccgtaat cgaaacattg gccgtcatcc ccggcttcag ccgcaaatcg
      121 cgattatcca cttccaccac cacgtcatag gtcacaacat tctggacaat aatcggggca
      181 ttccggattt gccttacgac acctcgaaag tccacctgtg gataagtgtc gacagtaaag
      241 agtgcctctt gtccctctcg gattcccccg atatcggact cactcacatt ggtatcaacc
      301 tgcattttcg ttaaatcgag ggcgatcaga aaaatattcg gtgtcgtgaa tcctgccgtc
      361 actgtctgcc ccacttccac attgcgtgca atcacaattc catttaccgg cgaccggatc
      421 acggtatatt ttaaatctaa atcggccgta tctaacaacg cctgagcctg tttgatctgg
      481 gctccggaga cttcaagctg agcctgggcc gattcatagg cggtggttgc catatccaga
      541 tcgttttgtg acactaactc tcgttgaaac aatgtctggg ttcgatcgag ttcccgtttc
      601 cgttgggcca actctgttcg cattttgctc cacatcgctt tagcattgac gaggttggcc
      661 gcagcctggt cccgtctggt ctgaaagaga gacgggtcaa ttcgcgcaat aacctccccc
      721 gccttcacaa cagagttaaa atcggcatgc aggctaatga tcttaccggt cacctgtgca
      781 ccaacttgga ccgaagttac cggattaatc gttccggtcg cacccacaat tgccacaatg
      841 gggccacgct caacaaccgc ggtgcgatat tgaatcggtg cactccgaac cccggtcaac
      901 cagaattgac gtgcggcaat caccgacaca atcactacca caccaatcca cagcaatttt
      961 ttcatccatg ctccattccc agacccattt atgctccgac ctccggactc cccattctta
     1021 gattggtaaa ggactgctcc attttaccat ttcttgatag ggaaaaatcg aaaatgagga
     1081 ctgccagaag ttcgaaatga aacgggtggt tcctcgaaca ccccccaaag catccgacag
     1141 aagagagatt gtacgccaac cagatcaggg aattcggctg gcgtggaagg gaaaaatcgg
     1201 taggtcatgg ggttcacgaa agagagaatc agtggccgat ccattttcag aatgaatggt
     1261 cccgacattc cttatagacc ttcacattcc ctaaggggtg ggttctatcc tcctctaccc
     1321 ctttgccgtg tcgtcttaac tccatcccgc atgggccatg cccgtcatag gtcttgtctg
     1381 ttccatctta actatgggat ttttcatcca cccggacctc cggtcgtccc aatttggaac
     1441 gggttacgca tcgtggatta gctctttttg cgatgtggag caaaaggcga acagttggtt
     1501 caataacgtt tcttatcttg ttgagaaaaa agatgggaac aggagaggat aagagagatt
     1561 tttggggcga cagttcggga aaaacccctc acactccccg ctcaaagtcc tttacgtatt
     1621 agctaaagtc ggaggagcca gcacacgcaa ccgagagagg aaggcggttg aatacgttct
     1681 caggccatgc gaaaccttat cgatccaatc aaaccaattg ctgatgagag aaaatgccca
     1741 ttcacacaac ctttataatc ggtagctaaa aacgggaaca atccatgaat gactcaaaac
     1801 ctaaaaacca ggaaaggagt gaaatcttca agaatggttt ccctcacttg cgaaatgaag
     1861 ttagtctttt gatcgaatca aaattccctt tcgtgccgtt ccaccacctg tctcaacatt
     1921 taaaaatgta acgccggatc gtaacccttc tggcacgacg acttccacgc gttcatcatc
     1981 cacaaacgaa aatgccttgg ccgggaccga gccaaaagtc acaccgtgca agcactcttt
     2041 tttctttccg aaattctttc cctctaccac aacactatca ccggttttca tttcgtccgg
     2101 ttttacatga tcaatttgag gacggacgct ggaatcacag atgggatcat ttttgacttt
     2161 ttgctcatca aaccccttaa tcgattccgg aatcacaaaa ccacccgact cttccacaga
     2221 ttgaccaggt actccttgcc cccaggaacc cgccaccccg agcagacaaa atccgaaaaa
     2281 aacaaggtgt ccaaataggt aggaaatcct caccaacatg acaaacctca caagttagag
     2341 tgttaggaaa ggtggccttt taccctcatc agccaaggca acattacacc atttcatcga
     2401 tcgtattttt cttattagat tcctgcggcc accccatcgt caatcgccaa tcacattgga
     2461 aaaaatttcg cagcctcggg gaacttcgaa aactccaaaa tttccatcgc aacaattttt
     2521 tgtgcttccc aacccattga actacctcag attccaatac cagtattcac agaatgcaga
     2581 caagaatttt cccttaacct taaaccaatt tgagctcata cccagacgaa tttttcttcg
     2641 gcacaagcaa atgaatctcc ctcctcgatc catgcgtcca gaccacgacg gataacctgg
     2701 ccaatctgat tgagcaaagt gcttctaggc tgatagaaag tagtatgtct gatgaggatg
     2761 actatctagg tgaatctgga ctctccaatg gatccgggcc gattggaatc cgcgcctttc
     2821 cgggagcccc tggaatggaa ggacaaagtt accttcgaac atttcgagtc tctccaggat
     2881 tggtgaggag gccctccata accgtgacga gacaattgga gccattagga tctatgtccc
     2941 gacaaaaatg tggtcgtgga aatcagcatg tccccctcac catcatcgga aaaattcacc
     3001 gaaggcattg cacaacaaat tttgaaaaaa tatgacaacc aatccgcaga tccttacagg
     3061 aagagcccct tatcccaatg tctgaatcag agcacgcatc tccggcacga gatccccacc
     3121 tccgctggaa cggcctgaca aagccgcatc gatacccgcc gccagcacag tcttggcctc
     3181 ctcataacgc ttcagtccag ccaatgcacg tcccaaagcc aaatgggagg cgacatgaat
     3241 gggatctaat gcgacagcaa cacgcaagtg ctccaccgct tcttcaagat gctccccttc
     3301 cttcagtagc ttgtctccaa gcccatatcg tcctagaaac ccatcgggtt ttttcgccac
     3361 catttggcga aatgtatcaa tatccatgct tttaccatcc tccccaaaaa tattgctgta
     3421 gctctcaatc ataccatccc ccttaaggga tcagccagac agaaggccac atgcaatgca
     3481 caccccctca cctctttgtg aaaagacgag aggaaaaatt cggccacacg aatcatatcc
     3541 catgccctct aagacaaatt cacgaactgg aaaaacaatg aatacccgtc ctgtcacctt
     3601 gatccatcaa agcccttctc ataattcggt gccttttgat ctgtatattc ataagcagcc
     3661 ggaatagatg agaaagatgg acttttttcc ccaagctaag gggagctgcg ttcgttttgg
     3721 tgagggtatg gacagcgcag gggtggtgat ggacgtgggt taacctttcc agggttcctg
     3781 ccaaaactgt taattacatc tgcatttcta ttcggccaag acccacagta gacttatgag
     3841 tggaaatgga cgatgcatgg ggaacgaaca aaagacacca caacgaatcc ggagacggca
     3901 ggtacccatc aaggcttgac tgatataatt cgtcgcaaac cctgcagttt aactccccag
     3961 caaacacata ctggcagttt aagggaaaat ttcttcccca ttttctccat cccgcttcac
     4021 agatcctgaa gatttccctg aaaacccgta gattttttca aaacttttcc tacgagagaa
     4081 ctcagttcag gaagatcatg cggctttttc agcactgcct cgaatccgga ctctaacgcc
     4141 tgcctacgaa tctcctgatt gggactccct gtcatcaaga caaatggggg gcaacaacca
     4201 ttacccacat attcaagatt tctccgtaac tgaataccat tcatcgcagg catttggaaa
     4261 tccgtcagga cgaggtcata atgactcatt tgaatatggg taaatgccca ggccacatct
     4321 gaaacttgac tacatagata cccttcgagt tccaaatatg attgataaag gtccaaaagt
     4381 tggcggtcat catcaataag cagaattctt tttgtgtttc ctcctgcccc agtgctgagg
     4441 aacttctcct gccgatttcc atcaggcaac atttccagag agtagggttc aagcataatg
     4501 gctctcatca ggtgaaggaa attttcccaa gcatcatatg tttttccatc catgctttcc
     4561 ccacccaatt ccatcagcac atgcaagagc gcatatacga caggaggata gtgcttgctt
     4621 ttaataccat atttcctgtg ccgttcccct aattgaacga gggaagaacg taattcctca
     4681 gggttttcca tgtgcaagac cagtgtgcgg aatccactta gaaacgccgc atattgttgg
     4741 gctaattggg cctgagagaa aaaggtctga aactccggaa atttatcaaa caacacatta
     4801 taaaaccgaa aaacgatctg ctctccgtga gaggccacaa tctcaaaact ttcctggatt
     4861 cgtgatatat caggagtggg aattcccgga gaaatattca atttaccatt cttcatggct
     4921 gcctttctga ggataaaaat ccttcctggg ttgacagatt tctaataaaa gagtcgcaga
     4981 catttatcgg tcaatatgtc tggagcgatc ggcttgatga gatagtccat cgctccctta
     5041 ataatggcaa gctgcatttc cgatcggtgt tcagagggtg acattacgat caccggcgga
     5101 agcgttgaag actgcgctaa ttgttccagc ataagaaggc ctcccatgac cggcatgcat
     5161 aaatcgagaa gtaccccatt gaccgtgtga tgttgaaggg tctcaacccc ttcctttcca
     5221 ttggcagcag taaggacctc aaatcccagg gcattcagac gatctctcag aaaaaggagg
     5281 acgtccgcat catcgtcgac aatgagaatg cgcttttgac ttttcatcgg tcgttacccg
     5341 tcatcgtatg gggggtcgtc ccatccatgc cgatccatcg gatggatgaa cgaatgaatg
     5401 gggaatgcat accaaacaca atgcctttac tgagagtcct tgacatcagt gattgtgagg
     5461 tgatcgtgta tcgcaccttg gcctatgacc gtgtgatggg gaagaacaat aatttcaccc
     5521 ctcatgatgg gatgaagcac gcaataataa ggataccgac cgggaggcag tgaagggatc
     5581 gtgaattcct gcccgggttg aacgccaccc gtatcaaatg cgcaaggccc accttttcga
     5641 caaccatcgt gtgtgacgct atgcggtgat gaagtcggat tcacaacatg aatcgagact
     5701 ccatcgggga caatagccaa agccggtcta taatagggtg cccaataatc cattgagacc
     5761 caaacatccg aaggttgggt ggaggccgat gcagggccac agattgatag aaataaaagg
     5821 aaatttccta acacagtcag agtgggagag gccgttgccc aattcaaatg tttcatctgt
     5881 tcacctcccg attccttact tctttcaacg ggttgttgtg cttaaccata tggtaatttt
     5941 caaacccata aagaatctat ttgatttgtg acccgttcaa ataaagattt cataaagatt
     6001 cctgatggcg ccatttcctg atttccgtgc agatcacacg gtcttgggca gtaacgaatt
     6061 ccatggaatg aagaagaacg gattgagtca ctcactagcc cacgagcgag taaaccggcc
     6121 atacattcaa tccggatgag cagggcaggg cctgccgatg cgtttttgga aggaggttgg
     6181 gctggaagag aggcgggcca aggcggccta agaaattacg agagaaacca caaagtgagg
     6241 aaatggttac agcaactggg aaggcaacag accatgattg atcaggaaga cctcacatgc
     6301 aatcggccaa tcaggggggg gtctcatctt cctcaaaatc ggtggcaaat cgatagccga
     6361 ctccaggatc ggtgagaata tactgaggtc gtgccgtgtt tttttccaat tttttcctta
     6421 aatttccaat aaagacacgt aaatattgag cctgctcgct atatcccgca ccccaaatgg
     6481 cccggagaat ggttccatgc gtcaaaactt ttccggaatt ctgaatgagg tacttcaaca
     6541 gatcatattc cgtcggggtt aatttcaccg cctggtttcg aatgcagaca tttcgactct
     6601 cgaaatcaac ctgtaaattt ccaaaggtga acacgggatc ggtgccgtaa attgaggatg
     6661 tccgccgcag gactgagcgg attctcgccg tcagttcttc catactaaac ggtttggtga
     6721 catagtcatc tgccccgact tcgagagctt caattttttt cgcttcttcc ccaatcgccg
     6781 aaaggataat gatcggaatc tggtaggttt cacggatctg gcgggtcagg tcgatgccat
     6841 ccattccggg aagcagtaaa tccacgagga ccaggtccgg aaggcggtgg ctcataattt
     6901 ggagggcctc ttctccggtt tcagccgtca ggacttcata tccttttgcc tcaagattga
     6961 tctgtaagga acgacgtatt tgccgctcat catcaacagc taatattcga gatccaagcg
     7021 ccataggtag accgcttact cctcttcccg gtcaaaattt atcatagggg gtgcatcagg
     7081 gatcggcaac gtaaagctaa tcgtcacgat tcgattctga gattcggccc aaattcgacc
     7141 accatgggct tcgaccatac ctttacagat cgccaatccg agaccggtac cacgaatgag
     7201 tcgtgtccgc ggacccttta accggtagaa tcggttgaaa atgctgacca attcttcccg
     7261 agggattcct tctccctcgc tggagaccct tacctccacc tggttaggcg tcatgcggcc
     7321 aacaatgctg accaaggagc ctgggggaga atatttaatg gcattatcta acaggttgat
     7381 caggacttgc tgaatttcca tcccatccac aaaaataggt gaaattttct ccccaaggtt
     7441 cacttgcaaa gatcggcttt tcaaaggcat ttccaaccgt ctgattgcgc cttcgaccaa
     7501 atcttccaat aaatgccatt cacgatgagg aaccagcgtt cccgcttcaa cctttgacat
     7561 gtccagaaga ttatcaacca atccatttaa aaaatcgatt tcctgattga taccctggag
     7621 aaattcctgc tgcacattgg atgcatcctg tctggaaagt tcaagcaatc cggcgatgga
     7681 ggccttgatg gccgtaagtg gagtccgtaa ttcgtgtgaa accaacgaca gaagagcggt
     7741 tttcagggca tcgctttgac gaaaggcctc aactttagcg gcttcacttt ttcgtaaatt
     7801 ttcctctacc tcttttcgtt caaacccatt cacgaatatt tctccggcca tttggaggag
     7861 cctgatgtcc tctttcgacc aggttttttc atgcctcact gaatcaaacc caagtaaccc
     7921 gaacaacttt ccccgtaata ccatgggcac aatcaccagc gattggattt gctgcctttg
     7981 aaatagctgc ttttcggcac tcgcctcaag cggaagattg gccacgcttg agatttgaaa
     8041 aaacgggccg gagataagac gttctgcaaa ccagggcgtt tcctgcattc ggagaccctg
     8101 aagttttgag agttggggct caatacccgt ctggcaccat tcatgggtat tgttaatggt
     8161 ctttccgtca tcttcaaaaa taaacacata acttcgatcc acccctccga aagtcccgat
     8221 agcttccaat gcccgattaa tccccgcatc caaatctttt gtttcaagac tgataaattg
     8281 ggttgaaata tccgtcacaa gattttcaat ttcaattcga tagcgcagag ccgtttctat
     8341 cctggagcgt tcctggattt catgttcaag ttcctgattg gccagtttga cctgtctcgt
     8401 tataagaatt ccgaaaatga tcactacggt accgatcaaa gattcccaaa cgatcatgtt
     8461 cgaaatcttt tcctgggaat ttctgctgta taacttgacc gctttgttgg ccacttcaat
     8521 caaccgggca tttaacgcca ggagcccgtc taactctaaa gaatatccgg gatgatcgga
     8581 tcgcgttttc aaaaaggtat cggcccgctg cataaattcg gccatgagta tttgttgctc
     8641 acctaatgcc tgtcgtatgt cctgtgatgg agggggagga aggaccgccg tatcaccact
     8701 ttcctgattt atcacggcct ttccaccgac gagtaaggca tccagatgtt gcttcagcat
     8761 cttttgcgtg aaccggtact cagcaggaat cccttgcgaa atcaataaaa tttcattcat
     8821 gagtcgttga gacaacatcc gctgttgtcc cgccagatct accatcacac tgtcgagctt
     8881 ttgattctga attgtggtaa tcgtatagag caggatgccg aacagggcta aaataaacac
     8941 ggtggagaga atcaataacg ctcgcccgat tgatcctgtt cctagccatg aacgttttgg
     9001 tggcttcacg actttccatc ctttgatgtc gaccctggtc gctcaatcca tacaataaag
     9061 gaatttcctg attttctcat cataccctaa actcttcgat atgtttccaa acacatgggt
     9121 tgcgggtatg tgagtcgcca gtgacctaag gaggcttacg aaaaagtttg gctccgactc
     9181 gatatttttg atcacacgat ttatactgtg ccccaataac gaatcattcg cctccctacc
     9241 ctctttattc ttcatctcgc ttaatgccag tccaagatct catacgattc ccgttattgc
     9301 tccatatctc tgaaatttca caggcctttc ccttcacggt tgtcttcttt cttggagata
     9361 ttcatgaata aacaatctca aatgcaccta gacaggcaga aggtaagcga ccctctcatt
     9421 gtccttgaag gctctgcgcc ccagtccggg aaatcgcctc tcccatctga tttaagaaaa
     9481 acaggtcttc acccggacaa ttggtatccg cttgcacgct ccaaagacct gaaaagggga
     9541 aaaatgctgg gagtgtcctt tgccggcaat cccatcgtgt tagtcagaac cgaaaacgaa
     9601 atggtctatg cgctcgaaga ccgatgcgcc catcggcagg taccgctcca ttgcggggtg
     9661 gtgcatggag agtatgtcca gtgtgggtat cattcctgga catttgaccg gacagggcga
     9721 tgcatgggtg taccatattt agaaaagaac caaccacgac cagcaggagt caggagtttt
     9781 ccctgtcgtg aagcctatgg gctggtgttt gtgtttccgg gggcagtaag ccgggtgaat
     9841 gagatcagat tcccggatgt atccttagca aatgatccac aatataaaac tcgatacctt
     9901 gaccgtcagg tgggctgcca ttactcattc atgcatgaga acctaatgga tatgaaccat
     9961 cagtttttgc atcgtcgact catgggtggg atccgaacgg tctttctgga acttcgagaa
    10021 cggccgcatg ccgtggaagt cgattacacc ttctctcgaa tcaaaggcgc acaaccactc
    10081 ggcgagaaac ttattttagg gagaaccaag gcatcagcag atgggccaaa gcatgatctg
    10141 atgaccatct gtaccaacta cccctatcaa acgctccagt tttggacagc cggaagtatt
    10201 caccctgcgt taaatctctg gaatgtgtat gttcccgtgg atccggcaca acgaatcaat
    10261 cacacctttg gattgatgat ggtcaggaaa ccttcaattc ccggcctcat ccacctcctg
    10321 tggccgttta ttgtgtggtt taccaatgga atctttgcac aagaccggtg gattgttgag
    10381 gaagaacaga aagcctttga tcgacagggc gccgactgga accaggaggt ctttccggtg
    10441 attcaaagct taagaagctt gttgggtcgc gagggcattg ccttatgagg actcctcccc
    10501 ccgccatgct gacaagattt ttccatgcgg cagccggatt gcaggtataa ctgacggcct
    10561 aggtttcggt agcttgatca gaccacatgg tagcgaaaag aggacggtgc ctcattcttt
    10621 tgcaggatcc tgcgacaggc ttccttgaga gatttcagat gaaacggttt cacaaaaaac
    10681 ccctgagccc cctcctgtag cagttgccgg agggctcgtt cgtccgacgc gccagacatc
    10741 atcatcaccg gcatttgatg tcccaaccat cgtaattcat ccaacatggt cctcccatcc
    10801 ataatgggca tatccatatc caatagaatg ccatccaccg tttggcggct tgccacagcc
    10861 aggccttccc atccatttct cgcaacgagg accttatatc cccattccgt gagcgcctga
    10921 cgtaacaggc tgcccacaga aggatcatca tccaccacca acagacgtgg acggacttcc
    10981 ggggtctcgg tctcatcaga ggacagactc cccgatgctg tattgagtgc ctgaagggtt
    11041 tgcaaacaag aggggcaata ggtatgcaca agatcgatct cagagaattt gagatgctcc
    11101 atcgacagga aggacgccaa tgtccgcatt cctttcgcac tggccggtgc gacagatgga
    11161 tccggcacgc gttcacacca tgcgcacgaa gaaacgaatt gggaggaagt cgaaggtgga
    11221 agacaatgcc gtaacacatt ggccttggag atgactccga caagattctg atcacccact
    11281 acaggcattc tctggatttg cgtctgaatc atcaaattca acaccatatc aagcgattca
    11341 ctttcatgca cataaaccgg cggggtcatg gtatcttcca ctctgagttc atccagcgaa
    11401 ttcaagcgtg aaacggcttc gaacagccgg agatccgaca cgatcccaag aattttgcgg
    11461 gtggaatcaa ccacaggcca tccacaatat tgaccggaga ggaattgcaa ggccagatcg
    11521 ttctcgaatt tcccacttct aagtggtaag gctttggggt ccattaaatc ttttgccaat
    11581 ggaggagatg aaggcaggtg gttcatgaga gggcctctct ttcaaaccgg tttcaaacag
    11641 gaaaccattg ggtaaaaagt tggtggggga tgaacaaata caaaaatgat tggaggaaca
    11701 aaagaacttc cgacttaatt aaataccagc aaaatgcagg ccaaaaacac tggggcgaag
    11761 ctcgagtaaa aaaaaccctt atatttcatg ctgtttccat aaaatatatg agattgggca
    11821 ttcctaccca ttaagttcac aaaagatcgg atgtattaaa agacacaaaa acaatagggt
    11881 aaatcataca atccgagatt ggaactatcc gacggaccca agaaaatgtt ccggcagaca
    11941 tcaggctata aaaaatattt gcagaatgat cgattatagg cgtgaggaat caagcagaaa
    12001 gttataaagc ccattccaac tcctccctgt ttttagcggc aactccgtta gagggaacct
    12061 cgagaacaag cgtttagcca ctgcttacgg tgttggtaac tgagggcaat aggaacgaaa
    12121 ctgaatcctg cagccataga tatcagaggt cccccttgtc atatccaccc aattccaaga
    12181 cagaatacgg cggctccagg tagaaggaga agcccccttg tctttacgaa aggtcagaaa
    12241 aataatccaa acctattttg ttcgatcaca atactcaaaa agtttggcct caggacgtgc
    12301 ccgagccaaa gcacggtttg gccccataag cccttgtcgc cagtgcacac atagccctca
    12361 tttgcgccca ctctcggggg tatgctacca gtgacctctc ccaacattga acgcagaaat
    12421 ggatcatgat gcacattacc gaacctaccg aaaaacgatt attgctcagg caccaccagg
    12481 gcgcgatcct gttcgtctcc tgttatgaat tgggtcacca acctcttggc atcgcccagc
    12541 caatgggatt tctccagcaa gccgggtttt cgccggttgg cctcgacatc gccgttgata
    12601 ccattgatga accggctcta aggctcgcca gatttgtcgg gatctcggtt cccatgcata
    12661 cagcgctcag acttggggtg cgggtagccg agctaattcg cgcaataaat ccagcctgcc
    12721 acatctgttt ctatggcctg tacgcctcga tcaatgcgga atatttgctt caggagttgg
    12781 ccgatactgt agtgggcgga gaatatgaaa tcccgctacg atcacttatt gaggcacttg
    12841 atcgcggtca ggatgccaac catcacgagg gagtgagctc ttcagaccgg gtcgttgttc
    12901 cctatttgaa gcggccctac tcaaccactt caacgacgtc ccaacccatc ccaagtcgga
    12961 caggactccc gatgttggac caatatgcca ggctcgaaga gaacgggaca gaacgactgg
    13021 tcgggtacgt cgagaccagt cgcggatgtc tccatctttg tctccattgc cccatcgtgc
    13081 cggtctatga agggcgattc ttccttgtcc cggaggccgt cgtcctcgag gacattcgaa
    13141 accaagtaca tgcaggcgca acgcatatca ctttcggcga tcctgatttt ttgaacgggc
    13201 cggggcattc cctgagtata gttcgcagga tgcatgagga atttccgcat ttgacctttg
    13261 atttcacagc gaagatcgaa cacctcttga agcaccgtcc tctgatgccg gatcttgaag
    13321 cacaggggtg cctgttcatc atctccgcag tggagtcgtt cagcaaccat gttttggaac
    13381 aattgcataa gggacataca ggagctgata tcatcacggc gttcaccatt ctgcgcgaag
    13441 ccggcatcac gcttcgcccg tcgctcgtgg ctttcacgcc ctggaccagc ctcgatgatt
    13501 accttcggat gttggacatg gtggaagacc acgatcttat cgacgcgacc gacccgatcc
    13561 agttgagcat tcgtctcttg gtgcctcccg gctcagctct tctcaccaag tccgacattc
    13621 agaggttt
//
LOCUS       DATIAL010000275         4462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1233542, whole genome shotgun sequence.
ACCESSION   DATIAL010000275 DATIAL010000000
VERSION     DATIAL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233542"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1295..1627
                     /locus_tag="VLA60_08455"
     CDS             1295..1627
                     /locus_tag="VLA60_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09432.1"
                     /translation="MLVRARWENGTRILMLSGRFDKFGRVPVETALAAGEGRPCHHVV
                     MDFSRVSSMDSAGIGRLLLLYHSLRRKGMALTVINPKPSVRALLDLAGLATIIPIMQE
                     KPEVRSVA"
     gene            1701..2084
                     /locus_tag="VLA60_08460"
     CDS             1701..2084
                     /locus_tag="VLA60_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09433.1"
                     /translation="MSTMPTQPTPIDDMHVITFGTCFDGSAEPALEAAVLQVQEIQGK
                     HIILDMEALTTLNSRALGKLFLTYHHLNRKHIRLSMVNPKPAVREMLTFVNFPQIVPI
                     YDSVDAVVALEQRQMESSGLPQQPG"
     gene            2257..2451
                     /locus_tag="VLA60_08465"
     CDS             2257..2451
                     /locus_tag="VLA60_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09434.1"
                     /translation="MSNLLFHEDPDIWLHAVLQLGGRANQISAQPLELGPWSKALHDQ
                     NPTVQKAVLQSLMRLTASNV"
     gene            2630..3730
                     /locus_tag="VLA60_08470"
     CDS             2630..3730
                     /locus_tag="VLA60_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09435.1"
                     /translation="MNESVVLDKVIKTHGLVKAVDHVSLSIQSGEFFSILGPSGSGKT
                     TILRLIAGLDRPDQGDIVIQQRVVTHDPPHKRPVNMVFQHYALFPHLSVFENVAFGLR
                     MKGEGQANIHKSVSHMLALVRLQGKEKRLPGQLSGGEQQRVALARALVNRPVVLLLDE
                     PLAALDQQLRQEMQGELKRLQREVQATFICVTHQQDEALMLSDRIAVMQGGKVLQVGR
                     PQEIYDRPVSSMVAQFIGLSNVLSGTIASCEGGWCRVDHEVLAPVKVRCSPHDSLQGA
                     VTVMVRPERIHVSSDMSPNGYDNRLQAIVHQVAFSGNEVVYHVRLRDEAIWMARVPIA
                     EAQARPLAVGQPVYVQWRAQEGLVLPAHPAPC"
     gene            3679..>4462
                     /locus_tag="VLA60_08475"
     CDS             3679..>4462
                     /locus_tag="VLA60_08475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF09436.1"
                     /translation="MARTGGTGLARPSGTMLNNRSDTSSAFPVKLSLLSASLFLGLFF
                     FLPLILVFGVSLASRGIYGGITWIPTWENYQRVFDPLVGVIFFRSLFLAGLTTFICAV
                     VGFPLAYCLARASRPWQLILLTLVMIPFWTNFLVRTYAWVLILRADGILNGLLIRLGL
                     LDEPLSLLFTDLSVFVGLVYGYLPFMVLPLVVAIERISPQLEDAAYDLYASMSGMFRH
                     VLLPLSTPGFLAGGVLVFIPSLGAFITPYLLGGGQNMMLGTFI"
BASE COUNT         1015 a         1031 c         1190 g         1226 t
ORIGIN      
        1 cttaggggct ttctctctta tcaagaagcc atttgactga atcctcccca atcgaatgtt
       61 tcgaaatgcc tgtaattcgt ttccctggag acaaaaaaga acggttgaag gcgtagatgc
      121 tcccttgaag ttctgcccga attagctaag ggcaacagcc aagtcacggt ggcagtcgga
      181 acacttagcc ccttctttcg agaccgcaac cagacgaatg aaatgtgcac accacataca
      241 gtaaaggtat cccggcccat tgaatcgaaa aggataggtg ggggagagct tcccccaatt
      301 gaccgcctct attggacacc aggagagtgt atgagacagc atcgcctaca aatcctttct
      361 caataatcgg tcttgatgat cacccctcta aattgagaga tgtgttttgt taacctcgga
      421 accagtgaag ggagaagact cctaaaaagg ctataaccgt ttcgatcatc cataatgttg
      481 tccagtccat gatgatgtcc tccttattcc tccattttct tctttttgtt cttggttatt
      541 tgtaaatagc aaccttaatg ccacgaattt ccgcgtatcc agctggccgg ctttcaggct
      601 actaactaat tgttttatta aaagaaacag ggaaggttgc gaaagggaag tctcgatctc
      661 accgtgcaga ccgaagtatc ggaattaata cagaccgtat catattcaca cagggaagct
      721 agtttggtga cgaggacgag attcaggagg ccctcctctc ggtctctcca agaaggagaa
      781 tgaaatttag tgtttaatag gtgtgagaag gggggcaaaa cggatgaacc taggggaggg
      841 gaaatgaatt ctgaaatcat gcattccagg gtatttgtag gaggagaagt agattgccct
      901 tttttttggc ttcccttggg ctttccagct attagcgcgc tgaccccagg cccgtcatcg
      961 cctttcttgt ctgtgggtga ccccattatt ccactgtagt gttgcatgtg ggggggcagg
     1021 aaactgcact gtccattaga tttcccatag aacgttttta cccattgggt aaggtctgct
     1081 tgacggttgg taggaggtcg agagccttga gtcatgcttt gttctctaca cacctgtttt
     1141 ttgaaggaaa ttttgatatg aaaaaggcgg agcccggaat attctcaaat gggagcgtgg
     1201 cacatctgtt gctgattatc tggtttgaag gatggcaaag ccgaggctat tcaatagcaa
     1261 aatgtgtcac ggacaataaa ggagggacag gtacatgttg gtacgagcac ggtgggagaa
     1321 cggaacgaga atcttgatgc tttctggtcg gttcgataaa tttggacggg tcccggtgga
     1381 aacggctttg gcggcgggag aaggacgacc ttgccatcat gtcgtcatgg atttttcacg
     1441 agtatcgtct atggatagtg caggcattgg aagactgttg ttgctctatc attcgcttcg
     1501 aaggaaaggc atggccttga cggtgatcaa tccgaagccc tctgttcggg cacttttgga
     1561 tctggcgggt ttagccacta ttattccgat tatgcaggaa aagcctgaag tccgatcggt
     1621 tgcctgatcc cctggggatg agaattgaat ttttttaagt tgcagaagag ggaaaccgaa
     1681 atggaaatta tcaaaccgtg atgtctacga tgcctaccca accgactccc attgacgata
     1741 tgcatgttat tacattcggg acatgctttg atgggtctgc ggaacctgct ttagaggcgg
     1801 ctgtcttaca agtgcaggaa atccaaggca aacacatcat tctagatatg gaagccctga
     1861 ccactctgaa tagtcgtgcc ttaggcaaat tatttctcac ctatcatcac ctcaatcgga
     1921 aacacattcg actcagtatg gtcaatccaa agccggccgt ccgtgaaatg ttaacatttg
     1981 tcaattttcc gcagatcgta cctatctatg attcggttga tgccgtggtg gccttagagc
     2041 aacgtcagat ggaatcttca ggtctgccac agcaaccggg gtgaaaaagg gtgtattggg
     2101 gccaagggta tctcagatga aaaaaggggt atgtaataat tagccaagac gagggaatga
     2161 agtgcctgaa cactcgttgg ccgcaagtag cgctcctttc ccaattgagg tgcggcatgt
     2221 ctggtaaaaa cagggcagct taccgtcgaa cgatccatgt ccaatcttct ctttcatgaa
     2281 gatccggaca tttggttgca tgccgtcctc caattgggag gacgagccaa tcaaatttct
     2341 gctcaacctc tggagcttgg gccttggagc aaggcacttc atgaccaaaa tccgacagtt
     2401 caaaaagcgg ttttgcaatc cctgatgcgc ctgaccgcct caaatgtcta aggcggatct
     2461 ccagccatcc cggccatccc cgtagatttt tctagcaccc cgctccactt catggaattg
     2521 cagtgggcat ggcaaggccg ctgaccctgc attgacttcc attttcctcc cttcctagaa
     2581 tcgtcaccct ggcgatttgt ccattccttc cgaaagggat ctctagctcg tgaatgaaag
     2641 cgtagtcctc gataaggtta ttaaaactca tggcctcgtg aaggcggtgg atcatgtctc
     2701 attgtctatc caaagcggag agtttttttc catattgggg ccgagtgggt ctgggaaaac
     2761 cacgatcctt cgattgattg ccggattaga tcggcccgat caaggagata tagtgattca
     2821 gcaacgtgtc gtcacccacg atccccctca taagcgaccc gtaaatatgg tgtttcagca
     2881 ttatgcgctt ttcccgcatc tgtcggtgtt tgagaatgtg gccttcggtt tacgcatgaa
     2941 aggagaaggc caggcgaata ttcacaagtc ggtgagtcac atgttggctc tggtgcgttt
     3001 gcaaggaaag gagaagcgtc ttccaggaca attgtcagga ggggagcagc agcgggtggc
     3061 gctcgcacga gccttagtga atcgtcccgt ggtactgctg ttggatgagc ccttggctgc
     3121 tttggaccag cagttacgtc aggagatgca aggagagctg aagcggttgc agcgtgaggt
     3181 gcaagcgaca tttatatgcg tgactcatca gcaggatgaa gctctcatgc tgtctgatcg
     3241 aattgcggtc atgcagggcg ggaaggtgtt gcaagtgggg aggcctcagg agatttatga
     3301 tcgccccgta tcttcgatgg tggctcagtt tatcggtctt tccaatgtct tgtccggtac
     3361 tatcgcctcc tgtgaaggtg gatggtgtcg ggttgaccat gaagtcttgg cacccgtgaa
     3421 ggtcaggtgt tccccgcatg attctctgca gggggccgtc acggttatgg ttcgcccaga
     3481 gcgcatacat gtatcctctg acatgtcccc caacggatat gacaataggc ttcaggcaat
     3541 tgtgcaccaa gtcgcctttt ccgggaatga agtggtgtat catgttcggc ttcgagacga
     3601 ggcgatctgg atggctcgtg tgccaatcgc cgaagcacag gccaggccat tggccgttgg
     3661 ccagccggtg tatgtgcaat ggcgcgcaca ggagggactg gtcttgcccg cccatccggc
     3721 accatgctga acaatcgttc tgacacatct tctgcttttc ctgtcaagct gtccctccta
     3781 tcggcaagtc tctttttagg cctgttcttt tttcttccgc tgatattggt ctttggtgta
     3841 agtctggcga gtcggggaat ttatggggga attacctgga tccccacctg ggaaaattat
     3901 caacgtgtct tcgatccact ggtgggggtg atatttttcc ggtctctttt cttggcggga
     3961 ttaaccactt tcatttgtgc ggtggtgggg ttccccttgg cgtattgtct ggccagggcc
     4021 tcccggccct ggcaactcat attgctgact cttgtgatga ttccattttg gacgaatttt
     4081 ttggttcgca cctatgcgtg ggtgctgatt cttcgagcgg acggtattct gaatggcctg
     4141 ttgatcaggc tgggattgct ggatgagccg ctttccctct tattcaccga tctgtcggta
     4201 ttcgtgggac tggtttatgg ctatctgccc tttatggtgc ttcctctggt ggtggccata
     4261 gaacgtattt ctccccaatt ggaggatgcg gcgtatgatt tatatgcgtc catgagcggg
     4321 atgtttcgac atgtgctgct tcctctcagt actccgggtt ttctggccgg tggggtgttg
     4381 gtctttattc cttccctcgg tgcctttatt acgccctatc tgttgggtgg aggacaaaac
     4441 atgatgctgg gaacattcat cc
//
LOCUS       DATIAL010000276         2539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_803706, whole genome shotgun sequence.
ACCESSION   DATIAL010000276 DATIAL010000000
VERSION     DATIAL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803706"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VLA60_08480"
     CDS             complement(<1..387)
                     /locus_tag="VLA60_08480"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HSF09437.1"
                     /translation="MGEFQYQEIFEHQADMTSYRKLTSDYVSTTTCDGQELLKVQTEA
                     LTLLAREAMDDIAHLLRPSHLAQLAKILKDPEASDNDRFVALELLKNANIASARVLPG
                     CQDTGTAIAMGYKGQQVLTTGDDAEAL"
     gene            745..1953
                     /gene="glgA"
                     /locus_tag="VLA60_08485"
     CDS             745..1953
                     /gene="glgA"
                     /locus_tag="VLA60_08485"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HSF09438.1"
                     /translation="MKVLMFTNEFPPYTYGGAGAHVEYLSRELSHLLPIEVRCFGDQR
                     LDQGNLKVHGIPLGDTPFTAPKPLHSVLGAVQRSWQMNAEGLGADLIHCHTWYTHFGG
                     IMAKLNYGIPLVITTHSLEPLRPWKREQLAGGYDFSLWVEKTALEMADAIIAVSEGTK
                     KDILRLFQVPEERVHVIHNGIDLQEFRNVQSQEVLTRYGIPAGQPYVLFVGRMTRQKG
                     ITYLIQALSYLDEEFPVVLCASSPDTPAMAAEMKEALDQISAHRKHIFWIPDILDKPS
                     LIELYSHAGVFCCPSIYEPFGIINLEAMACEVPVVASAVGGIPEVVVEDETGFLVPVD
                     QLEEAPFSPKDPERFAQDLADRLNQLMRDPELRAPMGKAGRRRAEELFGWDKIAKRTK
                     ALYETVLGES"
     gene            complement(2082..2315)
                     /locus_tag="VLA60_08490"
     CDS             complement(2082..2315)
                     /locus_tag="VLA60_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09439.1"
                     /translation="MHAEDELLESLRSFNDCEIRVYTRFATEWRDQRLTDGSQAEVSF
                     WNSVISMLVEERHRRKEEVQRLEAMFQTGHDPG"
BASE COUNT          531 a          583 c          725 g          700 t
ORIGIN      
        1 gagagcttcg gcatcatcgc ctgtggtgag aacctgttgc cctttatatc ccatggcgat
       61 ggccgttccg gtatcctggc agcctgggag cacacgggca gaggcgatgt tggcgttttt
      121 gagcagttct aacgcgacaa accggtcatt gtcggaggcc tcggggtctt tcagaatttt
      181 ggccaactgc gcgaggtggc ttggtcgcaa caagtgggcg atgtcatcca tggcttctct
      241 cgccagaagt gtgagggcct cggtttggac cttgaggagt tcctggccgt cacaggtggt
      301 ggtagagaca tagtccgacg tgagttttcg atagctggtc atatctgctt gatgttcaaa
      361 tatttcctgg tactggaatt cgcccatgtt tctcattccc cttttcgtct ataaccttaa
      421 agatctgatg atttatccaa atcgctgtcc ggtctgcttg gcaaactccc gttcatttga
      481 atctcaattg tgcgttttct ttcattgaaa cttcaagtga gtctcgagtt tttgattttc
      541 cggactttaa aagtcccctc cgcgattttg aaaggaactt gtgagggtga cagccttcag
      601 tcctcccggt agcgcgttat ctggatcagg tccgtctatt ttctccgtac gtttttttgg
      661 aaaggaaggt gacttcgtat gaattttctt ttaatgtagc aggctgctgg aagttttcac
      721 gatcataatg gagtgtcgtt gcccatgaaa gtcttgatgt tcaccaatga atttccccct
      781 tacacctatg gtggcgccgg ggcgcacgtg gagtatttga gtcgggaatt atcacatctt
      841 cttcccatcg aagtccgctg ttttggtgac caacgcctgg atcaaggcaa tttgaaggtg
      901 catgggattc ccttaggaga cactccgttt acggctccca agcccttgca ttccgttctt
      961 ggcgcggtgc agcgctcctg gcaaatgaat gccgaggggt tgggcgcgga cctgattcat
     1021 tgccatacct ggtataccca ttttggcggt attatggcca agctcaatta cggtattccg
     1081 ctggttatca ccacgcattc attggagccc ctccggccat ggaaacgaga gcagttagcc
     1141 ggcgggtatg atttttctct ctgggttgag aagacggcac tggagatggc tgatgccatt
     1201 attgccgttt ccgaaggcac caagaaagac attctcagat tatttcaggt tcccgaagag
     1261 cgggttcatg tcattcataa cgggatcgac cttcaggaat ttcggaatgt ccaaagccag
     1321 gaggtgttaa cccgatatgg gattcctgcc ggacaaccct atgtgttgtt tgtggggaga
     1381 atgacccggc agaagggtat tacctatttg attcaggctc tctcctattt ggatgaggag
     1441 tttcccgtgg tgttgtgcgc cagctctcct gatacaccgg ccatggccgc ggaaatgaaa
     1501 gaggcgctcg accagatttc ggcccatcga aagcatattt tttggatacc ggatatcctc
     1561 gacaaaccga gtctcattga attgtattct catgccgggg tcttttgttg tccgtctatt
     1621 tatgaaccat tcgggatcat caatttagag gctatggcct gtgaggtccc tgtcgtggct
     1681 tctgccgtcg gcggcattcc tgaggtcgtt gtggaggatg agacgggatt tttggtgccg
     1741 gttgaccaat tggaagaagc gccgtttagc ccgaaagatc ctgagcggtt tgcacaggac
     1801 ctggccgatc ggttgaatca acttatgaga gatcctgaat tacgggcgcc catggggaaa
     1861 gccggaagac ggcgggcgga agaacttttt ggctgggaca aaattgcgaa acgcacgaag
     1921 gcgctgtatg aaaccgttct tggggagagc tagggcgggg gctgtgagag ggacccatgt
     1981 gaaggggtat atgaaccgag gcctagtggt gggagttgag caggaagccc gggcaaatgg
     2041 gcattcctcg tgcacgcgtg agtggcgtga acccgggtct cttacccggg atcgtggccg
     2101 gtttgaaaca tggcctcaag acgctgcact tcctctttgc ggcggtgacg ttcttccacc
     2161 agcatggaga tcacggaatt ccagaaggag acttcagcct gtgacccgtc ggttaaccgt
     2221 tggtctcgcc attccgtcgc aaaacgggta tacactcgaa tttcgcaatc gttaaaactt
     2281 ctgagcgatt caagcagttc gtcttcagca tgcataagtc aactggcctt cctgaatttg
     2341 gagatgtaaa taaggattgt tggtcacagc gagcatgaga atctgtatcg gcggttcttt
     2401 gcgggttcct tagtgtgaga tgaattcttg ggaggaaatt tgtcgatgaa aaacatgtca
     2461 tccagtgtgc gcgcctgctt ccggtggatc gggcttggtg tgtggtgtgt catggtttgg
     2521 ggttgcgggg aagatcctc
//
LOCUS       DATIAL010000277         5133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1018345, whole genome shotgun sequence.
ACCESSION   DATIAL010000277 DATIAL010000000
VERSION     DATIAL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018345"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VLA60_08495"
     CDS             complement(<1..637)
                     /locus_tag="VLA60_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09440.1"
                     /translation="MKSDPLAEHSHLPSLLLVDDRQEILEQMKWGLKSSYMIYEADHR
                     AAAVDILQREKISLVTLDLGLPPDVGGVTEGLSALEQLLAVNPLVKVVVITGNQDRAN
                     ALKAIQLGAYDFMEKPVDLEVLKVVLQRAGYLAGLEKENQKLLEREAKRGFPEIIGSS
                     PVMVKVFDTIRRVAGSDISILIVGESGTGKELVARAIHQQSHRKDGPFIAIN"
     gene            complement(640..2829)
                     /locus_tag="VLA60_08500"
     CDS             complement(640..2829)
                     /locus_tag="VLA60_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09441.1"
                     /translation="MSVWAIPSILTAGLSLFLATVIFVKRKDGAHFSPLLLLMAVMIW
                     VHGLNGIQEVFPAHLIISKKLILIGEVLLPVLIGFVGFSLLHEFSANSVALGKGWWKI
                     IACGAALLAMVIVGTPDGFMQVNPEGEIVFRRPEGLAIWGFIVLASCVAIFQLERILQ
                     SFPDPLRYRLKYVLIGLGGLACISIAQAFHLVVVSVWRPEDVWAGGLASFSSLVIMAL
                     GLRRWRGLQVIQKIQISHQALYASLVVVCVSGYFVLVAMVTVVVQQTDWEVKESLGIV
                     LMFLAVMGLVVAMLSRRVRAEFLQVVSRHFFKAKYDYREKWLEVTETFSACQDVSELL
                     NRYLEWLSGTFGTSRVTIWKFFDVDGRYHQIRKERRRRKLTENDRSPNVSEPIPIPEN
                     HPLIHRLKGQQEPLLVQGGQRAAEEWREFLDVTHAHVCVPLVTVQGRLLGFCTLSQES
                     VHDVYDQDDLDLLRAIAHHVTMLLIQFELVEERSSSAKWEAVHRFSAFYLHDLKNLAS
                     SLSMVAQNAAQYGSNIEFQASAMQTVKNTSQRIIDLMGELSRQANEPNLSNGVPTESV
                     NVNLLIKDILAAVNGPGCQPNFSPGLEVPVLQLKREAIKQVLLNLILNARQAIEGQGT
                     IDISTAYDGKQVIVELVDTGGGMSAAQLQNVFQPFKSSKKNGLGVGLFQCKRIVEDHH
                     GVIHIESQEGRGTTVMITFPVQSADNASTMVFGVSQVGVEYDSRKNK"
     gene            complement(3586..4986)
                     /locus_tag="VLA60_08505"
     CDS             complement(3586..4986)
                     /locus_tag="VLA60_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF09442.1"
                     /translation="MADERILVVDDDPSLLTLLKMRLHSMGFVVTACGSGGDALSRVQ
                     EDPYDFAILDLRLPDADGLDLMEELHQHQGNLPILILTAHGCIPNAVEAMKKGAYGYL
                     TKPFDDKELLESIDKALTTKRMSQEIHRLQLLVNELYGLENVVARSAEMHTVLKQVAQ
                     VAKTDTTVCISGETGTGKEVIARVLHCNSPRASKPFMAVNCAAIPETLFESELFGHMK
                     GSFTGAHQNKRGLIQSADKGTLFLDEIGEMPLSLQGKLLRAIQNREVVPVGGQVPVKV
                     DVRILAATNKDLELAVREGRFREDLYYRIHVVPLWIPPLRQRREDIPSLAQFFLKRSI
                     EHHGKIVKGFSPEAMQALITYPWPGNVRELENMIERAVVMSPQDIITAEFLALSPQEQ
                     SKPGGLQPLTDAKEAFEKEYLQDLLKATQGNISKAAQIAGRYRADFYKLLKKYGLHPS
                     DRQDDAPSSNGQPGLQ"
BASE COUNT         1380 a         1375 c         1075 g         1303 t
ORIGIN      
        1 aattaatcgc gataaacggg ccatcctttc tgtgactttg ctgatgaatc gcccgagcca
       61 ctaactcctt tcctgtcccg ctttctccga cgattaaaat tgaaatatcc gaaccggcca
      121 ctcgccgaat ggtatcgaag actttaacca tgaccgggct gctcccgata atttcaggaa
      181 atccccgttt cgcctcacgc tccagaagtt tttggttttc tttttccagg ccggccaaat
      241 acccggcccg ctgcaaaaca acttttaaca cttccaaatc tacaggcttt tccataaaat
      301 catatgcgcc caattgaata gccttgagcg cattcgctcg atcttgattt cccgtgatga
      361 cgaccacctt caccaaagga ttcaccgcaa gcaattgttc caaggcagac agtccctctg
      421 tcaccccacc gacatcaggt ggcaaaccta aatctaaggt gaccagtgag atcttctccc
      481 gttgaagaat atcgacagct gctgcgcgat gatctgcctc atagatcata taggaggact
      541 ttaatcccca cttcatctgt tcaagaattt cctgccggtc atcaaccaga agaagtgaag
      601 gaagatgtga atgttcagca agcggatcag acttcatggt cacttgtttt ttctggagtc
      661 atattctacc ccgacttgag acacaccaaa caccatagta cttgcgttat cggcactttg
      721 tacgggaaat gtaatcatca ccgttgtgcc tcgcccttcc tggctctcaa tgtgaatgac
      781 gccatgatgg tcctctacaa ttcgtttaca ttgaaacagt cctacaccca atccattctt
      841 tttggagctt ttgaatggct gaaaaacatt ttggagctgg gccgcggaca ttccaccccc
      901 tgtatcgacc aattcaacga tcacctgctt gccatcatag gcggtggata tgtcaatcgt
      961 gccttgcccc tcaatcgcct ggcgcgcatt aaggatgaga tttaacagaa cctgtttgat
     1021 tgcctccctt ttgagctgaa ggaccggaac ctctagcccc ggggaaaaat tcggttggca
     1081 tccagggcca ttgaccgccg ccagaatgtc cttgatcaac agattcacat tcaccgactc
     1141 tgtcgggact ccattactca aattcggttc gttcgcctga cgagataatt ctcccattaa
     1201 atcgataatt cgctgagacg tatttttaac ggtctgcatg gccgatgcct gaaattcaat
     1261 attactcccg tattgcgcag cattctgagc caccatagat aaactcgacg ccaaattctt
     1321 taaatcatgt aaataaaaag ccgagaaccg atgcacggct tcccatttgg cggatgaact
     1381 acgctcttca accaactcaa attgaatgag gagcatcgtc acgtggtgcg caatggctcg
     1441 cagtaaatcc aaatcgtctt gatcgtagac gtcatgaacc gattcctgac taagcgtaca
     1501 aaacccaagc agtctgcctt gcaccgtcac caatggcaca cacacatggg cgtgagtgac
     1561 atcaagaaat tccctccatt cttcagccgc ccgctgcccc ccttgcacca acaagggttc
     1621 ttgctgtcct ttcaatcgat gaataagagg gtgattttca ggaatgggaa tcggctctga
     1681 cacattcggt gaccgatcat tctccgtcaa tttccttcga cgccgttcct ttcgtatttg
     1741 atgataccgt ccatcaacat caaaaaattt ccaaattgtg acccgagacg ttccaaatgt
     1801 tccactgagc cactcaaggt atcggtttaa aagttcggag acatcctgac aggccgaaaa
     1861 cgtttcagta acctctaacc atttctctcg ataatcatat ttcgccttaa aaaaatgacg
     1921 agaaacgact tgcagaaatt cggcacgaac acgcctggaa agcatggcga ccaccaaacc
     1981 cataacggca agaaacatga gaactattcc cagtgattcc ttgacctccc aatcggtttg
     2041 ttgcaccact acggtcacca tagcaacaag aacgaaatac ccactaacac acactacgac
     2101 cagggaagca tataatgcct ggtgactgat ttgaattttc tggatcactt gcagtcctcg
     2161 ccatcgcctc aatcccagtg ccatgataac cagagaacta aacgatgcaa gcccccctgc
     2221 ccacacatct tccggtcgcc acaccgagac tacgaccaaa tgaaaggcct gcgcgatgga
     2281 aatgcatgcc aaacctccca atccaatcag cacgtatttc aatcgatatc gtagaggatc
     2341 gggaaaagac tgaaggatac gttccagctg aaatatggcc acacaggagg ccaatacgat
     2401 aaacccccaa atcgccaatc cctctggtcg tctgaacaca atttccccct caggattgac
     2461 ttgcatgaat ccatctggag tacccacgat taccattgct aagagagcag ctccacacgc
     2521 aatgatcttc caccaacctt tgcccaatgc cacagaattc gcagaaaatt catgcaacaa
     2581 tgaaaaaccg acaaagccga tcaagaccgg cagaaggacc tcaccaatga gaataagttt
     2641 tttggagata atgagatggg ctggaaacac ttcttgtatc ccattcagcc catggaccca
     2701 aatcataacc gccattagca agagtaaagg ggaaaaatgt gccccatctt ttctctttac
     2761 aaatatgacc gtcgccaaaa acagggaaag tcctgccgtg agaatagagg gaatagccca
     2821 cacactcata cggaccctca atacctattg ggagttagag acagacacac gttcattttg
     2881 gggtgcttcc aaaggacaca gccgtttcgt tgttttgttt gatggagaca tggtgcttaa
     2941 tacatttttt aagtactcgg gtgcaccagg atttatggaa gaggcccccg actatctacg
     3001 ctggccatcg aaccctatgc gtatcaaagc tagattatct cttctggcca ggctcatgag
     3061 gattcagcca ccaagctact aaagcttggg ttaagagatg aagatatttc tcccgacagg
     3121 ttccgacacc tctatcatgg agagagatgg agcaccaccc tagcctgcct cacaggacat
     3181 taatcagaat gaaactgtag tattttttcg gaagatgagg agggagactg tagggttcgg
     3241 cctctcaaat cgacctcgcc tctcgcctga aaacgaaagc tagggttcga ttctgatgaa
     3301 tagaagtatt tttagcttct ccagctatag gacagagcaa tcccgcttca gccaataaat
     3361 tcaatcatgc agaaaaagga aaatgaggct atctgacagg accgacggtc ggatttcctg
     3421 ggatgacata ccgggaataa atgggctttt ccagccaatt cctgccgaga atctcgaagt
     3481 acgaaccagc atgagactac ttaactgccg tttttaaagt gaagcgaatc gtgtaccttc
     3541 ccgaccccat ggccagatat aagcctcttg actgctgtac tcaaattact ggagtcccgg
     3601 ttggccgttt gaagaaggcg catcatcctg tctatcggag ggatgcagtc catatttttt
     3661 gagcagttta taaaaatctg cccgatagcg cccggcaatt tgtgccgcct tagagatgtt
     3721 tccttgtgtg gctttcagca ggtcttggag gtactccttc tcaaatgcct ccttcgcatc
     3781 agttaatggc tgaaggccac ctggcttgct ttgttcttgt ggagaaagtg ccaggaattc
     3841 tgccgtaata atatcttgtg gagacatgac aacggcgcgt tcaatcatat tctcaagctc
     3901 tcgaacattc ccgggccatg gatacgtaat taaggcctgc atggcttccg gggaaaatcc
     3961 tttgacgatt ttaccgtgat gttctatgct ccgtttgaga aagaactggg cgagagaagg
     4021 aatatcctcc cgtcgttgcc gaaggggagg aatccacaag ggcacaacat gaatccgata
     4081 atacagatct tctcgaaatc gtccttcccg aacggctaac tccagatcct tattggttgc
     4141 ggccagaatt ctcacatcca ccttcacggg cacttgccct ccgacgggca ccacctctcg
     4201 attttgaatg gcccgcaaga gtttcccctg taatgacaaa ggcatttcac caatttcatc
     4261 tagaaataat gttcccttat cagcactttg gattaaaccc cgtttatttt gatgggcccc
     4321 ggtaaaggaa cccttcatat gcccaaacaa ctcactttcg aacaacgttt ctggaatagc
     4381 tgcacaattg acagccataa acggtttcga agctcgaggg ctattacaat gaagcacgcg
     4441 tgcaataact tctttcccgg ttccggtttc tccggaaatg cagacggtgg tatcggtctt
     4501 cgccacttga gccacctgct tcaacaccgt atgcatttcc gcactccggg ccacgacatt
     4561 ttcaagccca tataattcgt tcacaagcag ttgtagtcga tgaatctcct gactcattcg
     4621 tttggtggtg agggctttat caatactctc cagcaactct ttgtcatcaa acggtttggt
     4681 taaataccca tatgcgccct ttttcatggc ttccaccgca tttggaatac agccatgcgc
     4741 cgttaaaatc aggattggaa gattcccttg atgctgatgc aattcttcca taagatccaa
     4801 gccatcagca tccggcaatc gaagatccag aatggcaaaa tcatagggat cttcctgtac
     4861 gcggctcaac gcatcccccc cggagccaca cgccgtcacc acaaacccca tggaatgcag
     4921 gcgcattttc aggagggtca gtaaacttgg atcgtcatca acaacaagaa ttcgttcatc
     4981 agccatcaca attcctctat ttttttcctt caccttcatt tgggaattct ttctcctcgg
     5041 ctctttcgag tttgggggtt ggggccattt tttcagacgg gcgggtcggt ggggccttct
     5101 cgcggatttc ctgatcgata cgtcgtaaag cct
//
LOCUS       DATIAL010000278        21112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1233865, whole genome shotgun sequence.
ACCESSION   DATIAL010000278 DATIAL010000000
VERSION     DATIAL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 21112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21112
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233865"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="pgk"
                     /locus_tag="VLA60_08510"
     CDS             complement(<1..285)
                     /gene="pgk"
                     /locus_tag="VLA60_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HSF09443.1"
                     /translation="MKHLKKQTIDQVDLRNKRVLIRVDFNVPLDDSCQITDDSRIRAA
                     LPTINRAVDENAVVILCSHLGRPDGTINQALSLAPVAKRLQRLLNKPVEFV"
     gene            complement(355..1359)
                     /gene="gap"
                     /locus_tag="VLA60_08515"
     CDS             complement(355..1359)
                     /gene="gap"
                     /locus_tag="VLA60_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HSF09444.1"
                     /translation="MSIRVAINGFGRIGRNFFRTCFNDPAIEIVAINDLTDSKTLAHL
                     LTYDSIHGKFQADIQHGPDFLTVNGKQIQILAIKDPQALPWKEYQIDFVIESTGRFTD
                     RKSAGQHISAGAKRVIISAPAKDPDITIVLGVNDSDYNPQQHTIISNASCTTNCLAPV
                     SKVLLQEFGIKHGFMTTIHSYTNDQQLLDLPHKDLRRARAAALSMIPTSTGAAKALYL
                     VIPELKGKIDGMAIRVPTPNVSLIDLSVEVERDCDAATVNAAFQQAAQGPLKGYLQVS
                     EAPIVSSDLNGCSYSATVDAPLTSVINKRLVKVFAWYDNEWGYSCRLHDLINVLAQHR
                     "
     gene            complement(1652..2635)
                     /gene="trpS"
                     /locus_tag="VLA60_08520"
     CDS             complement(1652..2635)
                     /gene="trpS"
                     /locus_tag="VLA60_08520"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HSF09445.1"
                     /translation="MTTRVLSGMQPSGLMHLGNLLGALENWKSLQAQYECYFFVADWH
                     AMSTNYADTGRIKEFTQELLIDWLAAGIDPERATVFVQSSIPEHAILHLLFSMIVPIP
                     WLERNPTYKEKQEEIKEKDLSTYGFLGYPVLQAADILLYKPDIVPVGKDQLPHLELTR
                     EIGRRFNNLYSPVFPEPKEHLTQFPKVMGTDGRKMSKSYGNTINLSDTEPVVRQKLKT
                     MVTDPARVRRTDKGNPDVCPVFDFHKIYSSADVVKQVDQDCRTAAIGCIDCKRQVADA
                     MVERYQPMWDKRATILQHPEQTQEMVEEGRKRASHVAQETMQEVKTAMKVL"
     gene            complement(2656..3378)
                     /locus_tag="VLA60_08525"
     CDS             complement(2656..3378)
                     /locus_tag="VLA60_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSF09446.1"
                     /translation="MDSLSSFLYNLSFMLVPLMAAVVLHECAHGWVANFFGDQTAKNL
                     GRLTINPLPHIDLYGSIIVPLMLSLIPGGFVFGWAKPVPVNPAQLHNPRRDMAFVAAA
                     GPLMNLFLAIVSSLLLALFLYLDPTLQANWPPQPGVEPRRDLLGMILVPLTAMALSSM
                     IINIVLFSFNLLPVPPLDGSRVLRSLLPVRSAEVLNRLEPYGMFLILGLLMLNSYIPI
                     ISSFIGMVFQIFQRLFLPAFIT"
     gene            complement(3385..5328)
                     /locus_tag="VLA60_08530"
     CDS             complement(3385..5328)
                     /locus_tag="VLA60_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HSF09447.1"
                     /translation="MTTVKLKYRLLRPLLCLILASSGLLVDQGWWPFIGGGTNLAFAQ
                     QTGTSPLLDQAKLELEADNPAAAITILEKLIDSFPPPEILEEAYLLQATALKHDHQPT
                     DALTVLKQLLEEFPFSRSTNPARVLMAELYIELQNYEPALDQLYQALDYATDLDLRRS
                     LFQLIRQTELNNHNPLGAVKALLNEMGLADQTERLELESLTQTLILQQLDESALQELV
                     ESYSSRYPGDIATIRLIELHTAHGDEVLAERDIRGFLKRFPTHPYAQTAMALLQSFIS
                     KIKFHSHILAAALPFSGPMKTYGTDSLNGIRLALEIAKEQWGLTSVGLVVKDTTTLTA
                     PLRVEIQQMLDEFRPLAVVGPLLSREIQGLGRLPDDYATPFITPSSTLLNVRQFGSFW
                     FSTAMTSSVQAKRLVEYAILKLGYHRFGIIYPQTAYGREMADMFAKEVLHSGGEIIAS
                     EGYADDQTDIGEQLRHLKEKDLAKYGTTQTEKTRTGEDRLVYTPGIDAVFVPGQPVHL
                     ALIAAQLAFYDMNVPILGGNSWHNSELFRWAKHDLDGHIFVDGFFPNSPDPNIQMFVQ
                     RYRSQFQKEPSLFAFQAYDAATMVMETIRQGAQSGQGVWDQLVRRSDLPALSGFASFS
                     SAGILNRRLYLLQVKNKTFTQLN"
     gene            5582..6511
                     /gene="xerD"
                     /locus_tag="VLA60_08535"
     CDS             5582..6511
                     /gene="xerD"
                     /locus_tag="VLA60_08535"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HSF09448.1"
                     /translation="MQECIDRYLTMLQVEQGYAANTIESYRRDLKKLEIFFRTHEIAD
                     PTYLSKPLWFQFLNSLKAEGLSSSSIARCLASIRGFYKSFERGKDDPTFEGILKGTPK
                     QWSQLPKLLSEAEVTRLLNLSVRDRKEDLRDAAMVELLYATGLRVSELINLEMAHLNL
                     DVGFLQATGKRDKQRIVPIGDKARQLVVEYLQSSRPAFVKKRTSSALFLTRLGRAMSR
                     QCFWKILKNRTARAGITKPISPHMLRHSFATHLLDHGADLRSVQMMLGHASIATTQIY
                     THVEQARLKKVHDQYFPRKQRTRVIGAPHDAGS"
     gene            6632..6817
                     /locus_tag="VLA60_08540"
     CDS             6632..6817
                     /locus_tag="VLA60_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09449.1"
                     /translation="MKYRKQVHYVKGVFACPKCHQHFVQEKWLEEFRVRCHKCDYRGA
                     LTHVSVEEQMDEEIERR"
     gene            6894..7541
                     /locus_tag="VLA60_08545"
     CDS             6894..7541
                     /locus_tag="VLA60_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HSF09450.1"
                     /translation="MKVSRGNILWVVLFAFVWWSGCVQVSAQPSSPQASPQARDGLQV
                     LVAYHSLSGHTAEMAKSVKKGAESIPGTTVLLKTVGQVTADELFGSDAVIVGSPVYWS
                     NMSGEVKAFFDRWQFEFGVYPEWKMRNKVGAAFATGGQISSGKEVTMLTILAAMLGNK
                     MIVVSDGGAFGASATTEGDSPGINDAERADAWALGKRVAEVAWMIKRGQTIPGQS"
     gene            7552..8256
                     /locus_tag="VLA60_08550"
     CDS             7552..8256
                     /locus_tag="VLA60_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09451.1"
                     /translation="MSSMPGKSDDKWVIYWEREYATAFAPERLKLDFHPHRPLMTNMP
                     LPEQRELAASGYKVLPDDCGPVRAVGQYGWLIRSPADCRFRRKGTGFEWQSPPILPEE
                     RLLGYKSFSGMYVDTILNSGFPKLYCGIRFYYPKQVGMMMKDLPNHFYHFPSRTFSIW
                     EGIKTQEYKLTPNPYDFLPDHDAFVTNFLLQLDPSPDKTTTILRGDPIGVVFPVMLPK
                     HFTLEELKHPSEAPNT"
     gene            8479..9240
                     /locus_tag="VLA60_08555"
     CDS             8479..9240
                     /locus_tag="VLA60_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09452.1"
                     /translation="MKIQSKSDLILHQWQTLAKACDDGESQTRLAILWEHINRFPGRQ
                     AASAHAEIETAYFLAQAGFSVAFLEASGGRTADLECYEGTHRFFVEVTVIQSTQGPTR
                     KSPAVRLQPHQILESSDEFFEQALVRRLLSRMAEKARQLERYCAPVLLAVSVPDLPWR
                     KGRPQDIPPLDLQRLAAMLVGVVVDVPQFSAVLLTLWKAPAQELRNPIRIRQVSWVTR
                     PPGDARDPRIRLLAVNPVARYRLSSQELTSIKERM"
     gene            complement(9280..9600)
                     /locus_tag="VLA60_08560"
     CDS             complement(9280..9600)
                     /locus_tag="VLA60_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09453.1"
                     /translation="MANPKIEQFKKVLQMDPKDETMWFGLGKAYMNDENWKEAIPALE
                     QCIRVKPEYSAAYFALAQSLHQTGEFEKCQTICTQGIAVATKNGDLLVIKNLEALKAS
                     LPSS"
     gene            9810..10760
                     /locus_tag="VLA60_08565"
     CDS             9810..10760
                     /locus_tag="VLA60_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSF09454.1"
                     /translation="MSMGRVGYVSHPAYERHEMGRSHPESPERLRAIRAQLESSGTWS
                     RLHQVAPRRAERKWIELVHDSSYVESLERRSPSIGYVSLDPDTSMCPGTLEAAYLAVG
                     GGLAAADAIMNHEIDQAFCAVRPPGHHAEADQAMGFCFLNTIAIAARYLQQQYGLQRI
                     MIVDWDVHHGNGTQQAFYDDPAVLFFSTHQAPFYPGTGRATEIGAGQGKGLTINVPLS
                     GGQGDEEYREIFQKVLVPAAEHFQPECILISAGFDGHRDDPLASMALTEQGYADMTTI
                     VSKIAKKFASGRILSCLEGGYQLKALAGSVDRHLQALLDN"
     gene            10765..12087
                     /locus_tag="VLA60_08570"
     CDS             10765..12087
                     /locus_tag="VLA60_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HSF09455.1"
                     /translation="MKSKRSWGGKAWLLLLLVLGVGIYIFYTEIRPTVIFGLREDYAK
                     PIPYQQIPVGLQSLKAEECGSCHVEIYEEWKSSIHAKAFHDPFFQAYWKKDDNIWVCL
                     NCHTPLENQQPTLIQDLPRGRVEKAIQIPNYRYDAEYQQEGVTCAACHVRDGKILGPY
                     DDSAAPHPTQFDPSFRTTQVCYRCHNVVSGPMQFYNAGPCGTYPEYEGKFFMKEKGLI
                     CQSCHMPEVERPVAKGSPIRYGRRHLWRGGHDPDMVKRAVAVQVQADPPTPQPGDDVK
                     LTLTLINAGAGHKIPTGDPDRFFTVEFTVRDSNGTVVHEQSDTMGRWILWQPVIVEVY
                     DNRLLPLASRDYSFEYEMPENEKGWIVQARIRYHIQTDGQNQMLRDQYGLTADDPYVF
                     TIYEREFPLDATLSAVVQDQEPDLRVGCMAPSDGLTPHPPSDTSSLHS"
     gene            12099..12968
                     /locus_tag="VLA60_08575"
     CDS             12099..12968
                     /locus_tag="VLA60_08575"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSF09456.1"
                     /translation="MAENFFIDTESLAQNLGREDLVILDVRGRAAYSSHIPGAVHSTW
                     HEYSDPSAVAKGVLDPDLGRIEQRLRALGINQSSDVVIYCNPFDNWGDEGRMFWMLTY
                     LGHSSVRILDGGWVKWTAEQRPFEHEAIQPKPGDFTIKANPDLIVNKDGLKKLVKGPH
                     PDTIILDARSVEEYAGKEIDGLPRAGHIPSAINIPWNRFLQPDATVKPAEQVRKIFED
                     HGLRDNQEIMTYCLGGVRAAWVFCLLRYVGFPRVKVYPGSWWEWSRDFAAPAEKDVKL
                     LYRVTNQDQMKSS"
     gene            13044..13397
                     /gene="smbP"
                     /locus_tag="VLA60_08580"
     CDS             13044..13397
                     /gene="smbP"
                     /locus_tag="VLA60_08580"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HSF09457.1"
                     /translation="MKKQVVRGIGLFAILGLLIAGSWGSVALAGANPHVAEAIAHAQG
                     AVKHGEQGHADALVEHAQEALTHAQGAQKDVKNPHLDEGVHELMEAVEHGKAGHADVG
                     TKHAKSAVMHLKEVK"
     gene            13469..14269
                     /locus_tag="VLA60_08585"
     CDS             13469..14269
                     /locus_tag="VLA60_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HSF09458.1"
                     /translation="MSKISGGYFQFSPVFGEIQRNLETILNAIHNFEGHLLVFPELAL
                     SGYQFLSKEEVRELAGDIPSGPVIKTLEEAVRGRDLHVVVGLAERDHDRLYNSAVLIG
                     PKGYIGRYRKTHLFFEETLWFDPGDTGFLVWDIGRAKVGLLVCFDWFFPEAARSLAIK
                     GADILCHPSNLVLPYCPDAMVTRCLENGVFAITANRVGREQRGEKPALPFIGMSEIVA
                     PNGQILCRSALKDEEVGLYEMDMAEARDKRINPYNDLLKDRRPEWYVL"
     gene            14434..15195
                     /locus_tag="VLA60_08590"
     CDS             14434..15195
                     /locus_tag="VLA60_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF09459.1"
                     /translation="MNRWLVISVVLLLGLPGCMVSKSKYEASVADMESAKAELEKSRM
                     HQEALEEQIRNLKGLNEKVSTDLETMTTEVQRIKEGRKNEHTLLETRERELDQEKEQL
                     TSKLRVVVDQYQKVKAQNKALKETVLRYQKELKDTRESSIGGGAGAMKSSEKPEMSKT
                     ESAPSVNSSAIISSKPPVPKIVTPPTLPKIGGDLVNINKAPVSDLVLTLGLTREVAEE
                     VVSNRPYRLRGELVAKNIIPKATFDEIKDRITASP"
     gene            complement(15179..15391)
                     /locus_tag="VLA60_08595"
     CDS             complement(15179..15391)
                     /locus_tag="VLA60_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09460.1"
                     /translation="MLRNAGSSQNCSSSPALSHAERKASAAWTSGAYEEYVSKARQRP
                     ACAKPLRRRQGTPLAAFFTIPITEKP"
     gene            complement(15442..16191)
                     /locus_tag="VLA60_08600"
     CDS             complement(15442..16191)
                     /locus_tag="VLA60_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09461.1"
                     /translation="MNNPIDSEFTPQVPLVSIDNLCRSFQMGKQTVHVLNGITMTLAR
                     KEMVSIVGASGAGKSTLLHIMGTLDRPTSGKVLFEGKNMFRVSETALAGFRNQSIGFV
                     FQFHHLLPEFTALENTYLPALIQKIPKQQAMDAAKSLLSEVGLSHRLHHKPGELSGGE
                     QQRVAVARALIRHPDLVLADEPTGNLDTHTGDELFALLRKLNHTHGTAFVIVTHNEKL
                     SLQTDRLIQLQDGQIIEDRCLKDNSTTTERS"
     gene            complement(16184..17470)
                     /locus_tag="VLA60_08605"
     CDS             complement(16184..17470)
                     /locus_tag="VLA60_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF09462.1"
                     /translation="MTLPYEILVGLRYLRAKRRQRTISLNTFISITGITLGVAALIGT
                     LGIMTGFKEDLQSKILGTTAHVIVQERGSNDMKGYADLVDQIEAVPDVIAAAPFIFKQ
                     VLVTSKTAVQGIILRGIQPSQETRVTDLEANMKFGDLAGLTRPFTPSTSTSPDSVRDN
                     PTPSPTNPPGIILGKELAFRLGVFIGDTINVVSPVGPMTSLTMTPKIRPYTVVGVFES
                     GMFEYDSSLAYISLEEAQKFFNLGETVTGIQVKVNDVFLAQQIAQRLEATLGHIFIAR
                     DWMQLNRNLFSALKLEKTMMFLLLVLITLVASFNIVGTLTMIVNEKQREIAILKAMGA
                     TPGAIMRIFMLNGLVIGIVGTGIGVPLGYTFLWLIQNYWTFDASVYYISRIPVHILPL
                     DVLLVATSAILISFAATFYPSWQAAKLDPVSALRYE"
     gene            complement(17467..18954)
                     /gene="lysS"
                     /locus_tag="VLA60_08610"
     CDS             complement(17467..18954)
                     /gene="lysS"
                     /locus_tag="VLA60_08610"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HSF09463.1"
                     /translation="MEELNEQQQQRLEKLHALQDGGLHPFGTRFPTSHTLETILQSYT
                     DSTKEDLEGKPISCTVAGRIVAMRRFGKAAFAALQEEGHRLQVYLKKDLLGDQSYELS
                     QSFDLGDWIGVEGKLFRTKTDELTVEVHRLTFLTKGLRPLPEKWHGLTDIETRYRQRY
                     VDLIANPSVHQVFRTRSRIINIIRSFLTERDYLEVETPMMHSIPGGATARPFVTHHNT
                     LDVDLYLRVAPELYLKRLIVGGFRRVFEINRNFRNEGISTIHNPEFTMLEFYQAYADY
                     HDLMDLTEQLFHTLSLEVCGSPTVAYQGQSISLAPPWRRVPYLDSIVEVNQLDSEVLS
                     DHKKAVDAAKRLGVNCAPQASHVDILVNLFEATVEPTLIQPTFITDFPIEISPLARKK
                     EANPALTDRFELFIGGRELANGFSELNDPLDQRQRFEAQVALRDAGDDEAHYLDEDFI
                     RALEYGMPPTAGEGIGIDRLVMLLTNQASIRDVILFPQLRPDRPL"
     gene            complement(19076..20198)
                     /gene="prfB"
                     /locus_tag="VLA60_08615"
     CDS             complement(join(19076..20128,20130..20198))
                     /gene="prfB"
                     /locus_tag="VLA60_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155970262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HSF09464.1"
                     /translation="MLEDIRIRMEKINDHILEFRRYLDVPRLTTELEELERETSQPDF
                     WNNPRQGAKAGRRQAEIERQLARLHQLDQQRDDLLAILDLIGQQGDPELEAEWSKGIK
                     DIEATLEAWRLEQLLSGEHDQNNAILSINPGAGGTESQDWAQMLMRMYIRWAQERGYK
                     VETIDLQPGDEAGIKSVTLAIAGPLAYGYLHSEAGVHRLVRISPFDANKRRHTSFAAV
                     GVYPELDDDIDIVIDDKDLKVDTFRAGGAGGQNVNKVETAIRMTHLPTGTVVQCQNER
                     SQLQNRIGAMRVLKAKLYELEQQKKDAEFQNIVGEKKDIAWGSQIRSYVFQPYQMVKD
                     HRTNYESGNVAAVMDGALDPFIEAYLTMKASKASHMPAN"
     gene            complement(20219..>21112)
                     /gene="lnt"
                     /locus_tag="VLA60_08620"
     CDS             complement(20219..>21112)
                     /gene="lnt"
                     /locus_tag="VLA60_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HSF09465.1"
                     /translation="LVQANIDQAVKWNEAHRDETLRRYASLSQEAGRNTDLLIWPEAA
                     TPFLFEQEPAYQKQVLDIAKDTHNSLLFGSPTLRFQQDGRPYLYNSAFLVTAEGRASD
                     RYDKRHLVPFGEYIPLRSILFFLDKLVVGIGDFKSGQGVMTMQVPTVPPGSGPRFGVA
                     ICFEVIFPDLVRRMAREGANFLVTITNDAWFGDSAAPYQHFGMVVFRAVENHLGFARA
                     ANTGISGFIAPDGHILSQTSIFSEQAVTGLLPLRSSSPTFYTQFGDVFSWGCVIIAGI
                     LFTWCRFTTPLSTPKPRSLPI"
BASE COUNT         5097 a         4906 c         5827 g         5282 t
ORIGIN      
        1 aacaaattca accggtttat ttaatagtcg ctggagtcgc ttggccacgg gagccaggct
       61 gagtgcctgg ttgatggttc catccgggcg tcccaggtgg gaacagagaa tgacgacagc
      121 gttttcatct acagcacggt tgatggtggg aagggcggcc cgaatcctgg aatcgtccgt
      181 gatttggcag gagtcatcga ggggaacgtt aaaatccacg cgaatgagga cgcgtttgtt
      241 gcggaggtca acctgatcga tggtttgttt ttttaaatgc ttcatgcctt cctttccctt
      301 ccgatgccga tggttcgcgg gggaaagccg gataactttt tcgtgatgcg atatttaacg
      361 gtgttgagca agaacattga tcagatcgtg taagcggcac gaatatcccc attcattgtc
      421 ataccaggcg aacaccttaa cgagccgttt attaatgacg gaagtgagag gtgcgtccac
      481 ggtggctgag tatgaacacc cattaagatc acttgaaaca atgggggctt cagagacctg
      541 caaataaccc tttaacgggc cctgggcagc ttgttgaaag gccgcgttca ccgttgccgc
      601 atcacaatcg cgttcgactt caactgaaag gtcaatgagc gatacattgg gcgttggcac
      661 tcgaatggcc atgccatcga ttttcccttt caattccggg ataaccaggt acaacgcttt
      721 ggccgccccg gttgatgtag gaatcatgga taaggcggcc gcccgtgccc gtcgtaaatc
      781 cttatgaggc aaatcaagca gttgttgatc gttggtatac gaatgaatgg tggtcataaa
      841 tccgtgcttg atgccgaatt cctgtaaaag gactttcgac acaggagcaa gacaattggt
      901 cgtacaggag gcgttggata tgatggtgtg ttgttgagga ttgtagtcgg agtcattcac
      961 gcctaacacg atcgtgatat ccgggtcttt ggcaggtgct gaaataatga cccgtttggc
     1021 tccggctgaa atgtgttgtc ccgcactttt tctgtcggta aaacgacccg tggattcaat
     1081 gacgaaatcg atctggtatt ctttccacgg caaggcctga gggtctttga tggccaggat
     1141 ctgaatctgt ttcccattca ctgtcagaaa gtctggtccg tgttgaatat cggcttgaaa
     1201 ttttccatga atcgagtcat aggtcaaaag atgggccagg gtttttgagt cagtcaggtc
     1261 attgatggcc acaatctcga tggcaggatc attgaaacag gtccggaaaa aatttcttcc
     1321 gatgcgacca aacccattaa tagcaacgcg gatactcata gtttgatata cctaactaaa
     1381 tgaaaataag ggaatcttat ataggtatat gatcgtaatt tcacaatagc atgaagtcaa
     1441 gaaaatatcg cgagtctggc tcaagaatgt tcctcgtttg cccgggttgt gagagcaatg
     1501 aacctttgta aagccacgct attcccgtga acatggcttc tgtgtgcgca ctttgaacct
     1561 aggaacctga ggttgtgctc cttatcggcc gctttggtag tagaaaaatg ctacttcaga
     1621 aggtgctcga ggattgtgcg atttgaggtt gctagagaac tttcatcgcg gttttcactt
     1681 cctgcatcgt ctcctgtgcc acgtgagagg cgcgttttcg tccttcttca accatttcct
     1741 gggtctgttc aggatgttga agtattgtgg cccgtttgtc ccacataggt tggtagcgtt
     1801 ccaccatggc gtcggcaact tggcgcttgc aatcgatgca tccaatggca gcggtgcggc
     1861 aatcctggtc tacttgtttg acgacgtcgg cagatgaata aattttgtga aagtcgaata
     1921 ccgggcagac atcaggattg cccttgtcgg tccggcgaac gcgtgcagga tccgtcacca
     1981 tggttttgag cttttgtcga acgaccggct cggtatcgga aagattgatc gtattgccgt
     2041 aacttttgct cattttgcgt ccgtcggtgc ccatgacttt gggaaactgt gtgagatgtt
     2101 ccttgggttc gggaaatacc ggcgaataga gattgttgaa tcggcggcct atttcccgtg
     2161 tgagttccaa atggggaagc tgatccttgc cgaccgggac gatatcgggt ttgtataaca
     2221 gaatgtctgc cgcctggagc accggatagc ctaaaaatcc ataggtggaa agatcttttt
     2281 ctttgatttc ctcctgcttt tccttatacg taggatttcg ctccaaccag ggaatgggca
     2341 caatcataga gaacagcaaa tggagaatgg cgtgttcggg aatgctggat tggacaaaca
     2401 ccgtagcccg ttccggatca atgccggctg ccagccaatc aatcagcaat tcctgtgtga
     2461 attctttaat ccggccggtg tcggcataat tggtcgacat ggcatgccag tcggccacaa
     2521 aaaaataaca ttcgtattgg gcttgtaagg atttccaatt ttcaagcgcc ccaagaagat
     2581 tacctaaatg catcagcccg ctcggttgca tgccgctgag tactcgtgtg gtcatagtca
     2641 gtattccggt tctcgttaag ttatgaaagc aggtaaaaag aggcgttgaa atatctgaaa
     2701 caccattccg ataaagctgc tgattatggg gatatacgaa ttcagcatca agagtcctaa
     2761 aataagaaac atcccatagg gttccaaccg gtttaagact tccgcggatc tgaccgggag
     2821 gagactacgg agaacccggc tcccatccaa aggaggaacc ggtagaagat tgaacgaaaa
     2881 taacacgata ttaatgatca tggaagataa ggccatggcc gtgaggggaa ccaggatcat
     2941 gccaagaaga tcgcggcgtg gttcaacacc aggctgagga ggccagttgg cttggagggt
     3001 ggggtcgaga taaagaaata gggcgagcag caggctgctg acaatggcta aaaataaatt
     3061 catgagcggt cccgctgcgg ccacgaacgc catgtctcgt ctggggttat ggagttgtgc
     3121 aggattgact ggaacgggtt tggcccaacc aaacacaaac cctccaggta tcagggataa
     3181 catcagtggc acaatgatgc ttccatagag gtcgatgtgc gggaggggat tgatggtgag
     3241 tcgtcccagg tttttggccg tctgatctcc aaagaaattt gcgacccacc catgggcgca
     3301 ttcgtgtaat accactgcgg ccatgagagg aaccagcatg aaggagaggt tatagagaaa
     3361 agaggaaagg gaatccattt tgacttagtt gagttgggtg aaggtcttgt ttttgacctg
     3421 gaggaggtag agccttcggt tcagtatgcc ggcggaacta aaagaggcaa atccgctcaa
     3481 cgccggcaag tcagagcgtc gcacaagttg gtcccagacc ccttgtccgg attgagcgcc
     3541 ctggcggatg gtttccatca ccatggttgc cgcatcatag gcctggaagg caaagaggga
     3601 cggttccttt tgaaactgac tgcgataccg ctgaacaaac atctgaatat tcggatcagg
     3661 gctgttgggg aaaaatccat cgacaaaaat gtgaccgtcc agatcgtgtt tggcccaccg
     3721 aaataactca gagttatgcc agctattgcc gcctaaaatc ggcacattca tgtcataaaa
     3781 tgccagttgg gccgcaatga gggccaagtg cacgggctgt cccgggacaa acacggcgtc
     3841 aatcccgggt gtgtagacca agcgatcttc tccggttcgg gttttttcag tttgggtggt
     3901 accgtatttg gccagatctt tctccttcaa gtggcgtaat tgttcaccaa tatcggtctg
     3961 gtcatcagca tagccttctg aggcgataat ttccccgccg gagtgcaaca cttccttcgc
     4021 gaacatatca gccatttctc gtccatacgc tgtttgggga taaataatgc caaaacggtg
     4081 gtatcctagt tttaggatgg catattccac caggcgtttg gcctggacgg atgaggtcat
     4141 ggcggtacta aaccaaaatg atccaaactg ccgcacgttt aagagcgtgg atgaaggggt
     4201 gataaacggc gtggcatagt catcagggag tcggccgagt ccctgtatct cacgggacaa
     4261 taagggccct acgaccgcaa ggggccgaaa ttcatcaagc atttgttgta tctcgacgcg
     4321 taacggggca gtgagggttg tggtgtcttt tacgaccagc ccaaccgagg tcagtcccca
     4381 ttgttccttg gcaatttcga gagcgagtcg aatgccgttt aatgaatccg tgccataggt
     4441 tttcatggga ccggaaaaag gcagggcggc tgccaaaata tgcgaatgaa atttgatctt
     4501 gctgataaat gattgcagga gagccatcgc cgtttgagca taggggtggg taggaaagcg
     4561 tttgagaaat ccacggatat cccgttcggc gaggacctca tccccgtggg cggtatgcag
     4621 ttcaatcagc cggatggttg cgatgtcacc tggataccgg gaggaataac tctcaaccag
     4681 ttcctgcagc gcggattcgt ctaactgttg caaaatcaga gtttgagtga ggctttctaa
     4741 ctcgagccgt tccgtctgat cggccagccc catttcattt aacagggctt ttaccgcgcc
     4801 cagcgggttg tgattgttta actcggtttg acgaatcaat tggaataggg accggcgcag
     4861 gtccagatca gtggcataat ccaatgcctg atacaattga tccagggcag gctcatagtt
     4921 ctgtaattcg atatagagtt cggccattaa gacccgagcc gggttcgtgg aacgcgaaaa
     4981 gggaaattct tccaggagtt gcttcaaaac cgtaagagca tcagtcggct ggtgatcatg
     5041 tttcagggcg gtggcttgca agagataggc ttcttccagg atctctggag gagggaatga
     5101 atcaatcaac ttctcgagaa tcgtaatggc ggctgcagga ttatcggctt ccagctccaa
     5161 ttttgcctga tccagaagag gggaagttcc agtttgttga gcaaatgcga ggttggtgcc
     5221 tccgccgatg aagggccacc atccctgatc caccagcaat cccgagcttg ccaggatgag
     5281 acacaaaagg ggtcgcagca atcgatattt gagtttgaca gtcgtcaaag ggaatttacc
     5341 taaacgcctt ggaaatggaa gggattgttc gtatggaaac tgggcaacac ccgaatactt
     5401 ggatggataa ttcacaagca gaatacggtg acgataatgc ttttcttgtt tagtatacca
     5461 ggaagttcgc ttaccaaaat agaagaacgc aagaaaatct ctggatagga ttatggccgg
     5521 tgggggcatg aaaaggtttg aggtacttcc atcatgaaga tgagggtggc gtaaagagct
     5581 gatgcaggaa tgtattgatc gttatctcac catgctgcaa gtcgagcagg gctatgcggc
     5641 gaataccatt gagagctatc gacgggacct caagaaactc gagatttttt ttcgaaccca
     5701 cgagattgcc gatcccacat atctttccaa gccactgtgg tttcaatttt taaacagttt
     5761 gaaagccgaa gggctgtcct cctcttcaat tgccaggtgc ctggcttcta tccggggttt
     5821 ctataagtcg tttgagaggg gaaaggatga tccgaccttt gagggtatat tgaaagggac
     5881 gccaaagcaa tggagtcaat tgccaaagtt gctgtccgaa gccgaagtga ccagattgct
     5941 taacttgtcc gtgagggata gaaaggaaga tctacgtgat gccgccatgg tggaactgct
     6001 ctatgccacc ggtcttcggg tatcggaact tatcaatttg gaaatggcgc atctcaatct
     6061 ggatgtgggg tttttgcaag cgactggcaa gcgggataaa caacgaattg ttccgatcgg
     6121 agacaaggca cgccagctgg ttgtggaata tctacagtct tctcgcccgg cctttgtcaa
     6181 aaagcgaacc tcttcggcgt tatttcttac ccggctgggt cgggccatga gccggcaatg
     6241 tttttggaaa attctcaaga atcgaacggc tcgggcaggc atcaccaagc ctatttcacc
     6301 gcacatgctt cgccattcct ttgccacgca tttattggat catggtgccg atcttcgttc
     6361 ggtccaaatg atgcttggcc atgccagcat tgccacgaca caaatctata ctcatgtgga
     6421 gcaagcccgc ctcaaaaagg ttcatgacca atactttccc cgtaaacaac gtacgagggt
     6481 cattggagcc ccccatgatg ctgggtcttg agcaggtatt tgcccgggtg cccccctgcg
     6541 ggcatggcct gctcccaatt tgcaattgga aaaaggcctg tggcaggcta ccaccgagct
     6601 tttcttttat aaatttttgg aggggttggt tatgaagtat agaaagcagg tgcattatgt
     6661 gaagggggtg tttgcgtgcc caaaatgtca tcaacatttt gtgcaagaga agtggttgga
     6721 ggaattccgg gtacggtgtc ataagtgcga ttatcgaggg gcattgacgc atgtatcggt
     6781 ggaagagcaa atggacgaag aaattgaacg acgatagcta agattgattg tctgctagat
     6841 tgggtttttt aggttcaaat agtcggtgcc tttattccaa ttcaattctc ctcatgaaag
     6901 tatctcgggg aaatatcctg tgggtggtgc tgtttgcatt tgtttggtgg agtggttgtg
     6961 tgcaagtctc cgctcaacct tcatctcccc aagcatctcc tcaagcgagg gatgggcttc
     7021 aggtcttagt cgcctatcat tccctgtccg gtcatacggc cgagatggcc aagagtgtga
     7081 aaaaaggagc tgaatcgata ccaggaacca ccgtcctact gaagaccgtg gggcaggtga
     7141 ccgccgatga gttgtttggc agcgatgccg tcatcgtggg ttcgcccgtc tattggtcga
     7201 atatgtcagg tgaagtcaaa gctttttttg accggtggca gtttgaattt ggcgtgtatc
     7261 ctgaatggaa aatgcgtaat aaggttggag cagcctttgc caccggtggg caaatatcca
     7321 gcggaaaaga ggtgacgatg ttgaccatcc ttgccgccat gcttgggaac aagatgattg
     7381 tcgtgagtga cggaggagcg ttcggcgctt cagcgacgac tgaaggcgat agccctggaa
     7441 ttaatgatgc ggagcgtgcc gatgcatggg cgttgggaaa gcgcgtcgct gaagtggcgt
     7501 ggatgatcaa gcgagggcag acgatcccag ggcaatcctg aagggcattc catgagttcg
     7561 atgccgggaa aatcagacga taaatgggtc atttattggg agcgggagta tgccaccgcc
     7621 tttgctccgg aacgactcaa gctggatttt catccccacc gccccttgat gaccaacatg
     7681 ccgttgcctg agcaacggga gttggccgcc tccggatata aggtccttcc tgatgattgt
     7741 gggccggtta gggccgtagg gcaatatggc tggcttatcc gttcacccgc tgattgccgg
     7801 tttcgtagga aaggcactgg ttttgaatgg cagtctccgc ccattcttcc tgaagagcgg
     7861 ctcttggggt ataagtcatt ttcgggaatg tatgtcgata cgattttgaa tagtggcttt
     7921 cccaagctct attgtggcat tcgtttttat tatcccaaac aagtcggcat gatgatgaag
     7981 gaccttccca atcattttta tcatttcccc agccggacct tctccatctg ggaagggatc
     8041 aaaacgcaag aatataaact gaccccgaat ccctatgatt ttcttcctga ccacgatgcc
     8101 tttgtgacaa attttctgct gcaattggac ccttctccgg acaagaccac gactattctg
     8161 cgtggcgatc ccataggtgt ggtgttcccg gtcatgctcc cgaaacattt tacactcgaa
     8221 gagttaaagc atccttcaga agcgcccaac acataatgcc gagcaggccc ccggcttgag
     8281 cctcttgcgt tcttttccct ttaaataata tgcagtgatg cctgtgaggt ataagctatt
     8341 gcccgatttt cctgcctaag ggaaagcctg ttgccgcttc aaattgatat actggctgaa
     8401 tgtcggcggg taggcatggg ccccgattgc cgaaaaacgc attggttggt tcgatttccg
     8461 tgctcatgag ggaggagcgt gaagatacaa tccaagtctg atttgattct ccaccaatgg
     8521 cagactcttg ccaaagcctg tgatgacgga gaaagtcaga ctcgactggc gatattgtgg
     8581 gagcacatta atcggtttcc gggcaggcag gcggcgtccg cccatgccga gattgagacc
     8641 gcgtattttc tcgcacaagc gggcttttcc gtggcatttt tagaagcctc tggcggtcga
     8701 acggcggact tggagtgtta tgaaggtacg catcgatttt ttgtggaggt cacggttatc
     8761 caatcaaccc aaggacctac tcgcaaatca ccggcggtgc gtctacaacc tcaccagatt
     8821 ttggaatcct ctgatgagtt ttttgaacag gctttggtca ggcgattgtt gtcacgcatg
     8881 gccgaaaaag ccaggcaatt agaacggtac tgtgcacccg tgttgctggc ggttagtgtt
     8941 cccgatttac catggaggaa agggcgccct caggacattc cccccttgga tcttcagcga
     9001 ttggctgcga tgttggtcgg agtggtcgtc gacgtccctc agttcagtgc ggtcctgctg
     9061 accctatgga aggctccggc gcaggaatta cgtaatccca ttcgtattcg ccaggtctcg
     9121 tgggtcactc ggccaccagg ggatgcacgc gatcctcgaa ttcgattgtt ggcagtgaac
     9181 ccggttgctc gatatcgcct tagctctcaa gaactcacga gtatcaagga acggatgtga
     9241 agcgaagctg cggggaagga ttggagcagg taccagcggt taggatgaag gcagggacgc
     9301 tttgagggct tccaggtttt taatgacgag gagatcacca ttcttggttg ccacggcgat
     9361 gccttgggtg catattgtct ggcatttttc aaattcgcct gtctggtgaa gcgattgagc
     9421 caacgcaaaa taggcggctg aatactcagg tttcacccga atgcattgct ccaaggcggg
     9481 aatcgcctct ttccaatttt cgtcattcat gtaggctttc cccaggccga accacatggt
     9541 ttcgtccttc gggtccattt ggagcacttt cttgaattgt tcgatcttag gattggccat
     9601 ggttgagatc cgatcagaaa taaaggtgac ggaacgtgaa tgaattcgag cctatcatgc
     9661 gggtatatgc gttgtctaag ccattcccct atgctaggat ctgatacgtt tttccttccg
     9721 gttcggcagt attcgcaagt attgtttggc cgacattgtt gatcgagttt ttcctctgtg
     9781 catgatttcc tattccattc tgagagtgta tgagtatggg acgtgttgga tacgtttctc
     9841 atccggccta tgaacgccat gagatgggca gaagccatcc ggagtcacct gaacggcttc
     9901 gtgccatacg cgcgcaatta gaaagttcgg gtacgtggtc ccgactgcat caggtcgccc
     9961 cacgacgggc cgaacgaaaa tggattgaac tcgtccacga ctcgtcctat gtggagagcc
    10021 ttgaacgacg ttccccctca attggttatg tttccctgga tcctgatacg tcgatgtgtc
    10081 caggaacgct ggaggccgcc tatctggcgg tcggcggggg gttggcggcg gcggatgcga
    10141 tcatgaacca cgagattgat caggcgtttt gcgccgtgcg gccgcctggg catcacgccg
    10201 aggcggatca agcgatgggg ttttgtttcc ttaataccat cgcgattgcc gcccgttatc
    10261 ttcagcaaca atatggatta cagaggatta tgattgtgga ttgggatgtg catcatggca
    10321 atggcacgca acaggccttc tatgatgatc ccgctgtcct tttttttagt acccaccagg
    10381 ctccatttta tcccgggacg ggccgggcga cggaaatcgg ggcaggtcaa gggaaaggtc
    10441 tgaccatcaa tgtgccgctc tcgggaggac aaggggatga ggagtatcgc gaaatattcc
    10501 agaaggtctt ggtcccggcc gccgaacact ttcaacctga atgtattctg atttccgccg
    10561 gatttgacgg tcaccgggat gacccattgg cgagtatggc cttaacggag caagggtatg
    10621 ccgatatgac aacgattgtc tcgaagattg caaaaaaatt tgcaagtggg cgaatcttgt
    10681 catgtttaga aggtggctat caactcaagg cgttggcggg atccgtggac cgccatctac
    10741 aggctctgct ggataattaa cgtgatgaag tcaaagcgat catggggagg aaaagcgtgg
    10801 ctgttgctcc tgttggtgct gggggtaggg atctacattt tttatacgga gattcgtccc
    10861 accgtcatat tcggcttacg agaggattac gcgaaaccca ttccctatca acagattcca
    10921 gtgggattgc agagcttgaa agcggaagaa tgtggaagtt gtcatgtcga aatctacgaa
    10981 gagtggaaat ccagtattca tgccaaggcc tttcacgatc cgttttttca agcctattgg
    11041 aaaaaagatg acaatatctg ggtctgtttg aattgccata cgccattgga aaatcagcaa
    11101 ccgactctta ttcaagacct tccccgtggg cgtgtcgaaa aagccattca aattcctaat
    11161 taccggtatg atgcggaata tcaacaagaa ggtgtgacgt gcgcagcctg tcatgttcgg
    11221 gatgggaaga ttctcggtcc atatgacgat tcggcggcgc cccatcctac ccaatttgat
    11281 ccttccttca gaacgaccca agtctgttat cgctgccata atgtcgtttc gggacccatg
    11341 caattctaca atgcggggcc ttgtggcacg tatcctgagt atgaaggcaa gtttttcatg
    11401 aaggaaaagg gactgatttg tcagagttgt catatgccgg aagtcgagcg gccggttgcc
    11461 aagggtagcc ctatccgata cggtcgacgg catctctggc ggggaggtca tgatccggac
    11521 atggttaagc gagcggtcgc tgtccaagtg caggcagacc ctcccacacc ccaaccaggt
    11581 gacgatgtca aattgacgct gaccctgatt aatgcggggg ctgggcacaa aatcccgaca
    11641 ggggaccctg accggttttt taccgttgaa tttacggtgc gtgattcgaa cggcacggtg
    11701 gtgcatgagc aatctgatac catgggtcgt tggatcctct ggcagcccgt gattgtggaa
    11761 gtatacgata accgattgct gccccttgcc agccgggatt attcctttga atatgaaatg
    11821 ccggaaaatg aaaaagggtg gatcgttcag gcgcggattc ggtatcatat tcagaccgat
    11881 ggtcaaaatc agatgttgag agatcagtat ggtctgacgg ctgatgatcc atatgtcttt
    11941 acgatctatg aacgggagtt ccccctggat gcgacccttt ctgcggttgt acaggatcag
    12001 gaacccgatc ttcgggtagg atgtatggca ccctccgatg ggcttactcc tcatcccccg
    12061 tcagatacgt cttcacttca ttcataaagg aaagcactgt ggccgaaaat tttttcattg
    12121 ataccgaatc acttgcccaa aacctgggcc gcgaagacct ggttatcctt gatgtacgag
    12181 gccgtgcggc ctattcctca catattcccg gtgccgtcca tagcacctgg catgagtata
    12241 gcgatccgtc tgccgtagca aagggggtct tagatcctga ccttgggaga attgaacagc
    12301 gtcttcgagc gctcggtatt aaccagtcca gcgatgtggt tatttattgc aatccattcg
    12361 ataattgggg tgatgaggga cgcatgtttt ggatgttgac gtatctggga cattccagcg
    12421 tccgaattct ggacgggggg tgggtcaagt ggactgcaga gcagcgtccc tttgaacatg
    12481 aagcgattca acccaagccg ggcgatttta ctatcaaggc caatccggac ctcattgtga
    12541 ataaggatgg attgaaaaaa ctggtcaaag gaccccatcc ggataccatc attctggatg
    12601 ccagaagcgt tgaggaatat gcggggaagg agatcgatgg gttgcctcgg gccggccata
    12661 ttccttctgc catcaatatc ccgtggaatc ggtttttaca accggatgcc acggtgaaac
    12721 ctgcagagca ggtcaggaaa atatttgagg atcacgggct acgggacaac caggaaatca
    12781 tgacatattg cctgggtggg gtacgggcgg cttgggtgtt ttgtctgttg cgatatgtcg
    12841 ggtttccacg agtgaaagtc tatccagggt cttggtggga atggtcacgt gatttcgcgg
    12901 caccggcgga aaaagacgtg aagttactat atcgggtcac caatcaggac caaatgaaat
    12961 catcttgatc ttgtgtgtga gggatgagat tataacttac tctctcagtt taatattaat
    13021 ttactttttc aaggaggtca gggatgaaaa agcaggttgt taggggaatt ggtctattcg
    13081 ctattctggg gttactgatt gcggggtcgt gggggtctgt cgcccttgca ggagccaatc
    13141 cacatgtggc tgaagcgatt gcccatgccc agggagctgt gaaacatggc gagcagggac
    13201 atgctgatgc gttggttgag catgcccaag aagccttgac ccacgcccaa ggtgcccaaa
    13261 aggacgtgaa aaatcctcat ttagacgaag gggttcacga acttatggaa gcggtggaac
    13321 atggtaaagc cggtcatgcc gatgtcggga ctaagcatgc aaaaagcgca gtgatgcatt
    13381 tgaaagaggt taagtaatca taaagcctta atctgatgaa agaagcgggt agagttcacc
    13441 tctgcccgct tttttttgtg gggtggtgat gtccaagatc agtggagggt attttcaatt
    13501 ttctccggtg tttggggaaa tccaacgtaa tctagaaacc atcctgaacg ccatccataa
    13561 ctttgaaggg catcttcttg tttttcccga acttgccctc tcgggatatc aatttctttc
    13621 taaagaggaa gtccgggaac tggctggaga cattccttcc ggtcccgtta ttaaaaccct
    13681 ggaggaagcc gttcggggac gtgacctgca cgtcgtggtc gggttggctg aacgagacca
    13741 tgaccggtta tataattcag ccgtgctgat cggcccgaaa gggtatatcg ggagataccg
    13801 gaaaacccat ttattttttg aggaaaccct ctggtttgat cctggagata cgggatttct
    13861 tgtctgggat attggaaggg ctaaggtggg ccttctcgtc tgttttgatt ggtttttccc
    13921 cgaagccgca agaagtctgg ccattaaggg cgccgatatt ttatgccacc ccagcaattt
    13981 agtattgccc tattgtcctg atgccatggt gacgagatgc ttggaaaacg gggtgtttgc
    14041 cataacagcc aatcgagtgg ggcgggaaca gcgtggggaa aaacccgcat tgccatttat
    14101 tggcatgagt gagattgtgg cccccaatgg acagattttg tgtcggtctg cccttaaaga
    14161 cgaagaggtt ggtctgtacg agatggatat ggcggaagcc cgcgacaaac gaatcaatcc
    14221 ctataacgat ttgctcaaag accggcgtcc tgaatggtat gttttgtagg cggcaagtag
    14281 ttggaatcca caagccaggg cgccgctagg cttgacataa ccgtgaggat gccataaatc
    14341 ctgaaataaa gggtgtaaac ccaagagccg gacaggggca ggaagagttt ttcaaggatg
    14401 aatctttcta ttggcaggga cgaggaggat attatgaaca ggtggttggt tatttcggtc
    14461 gtgcttctgt tgggattgcc agggtgcatg gtttcgaagt cgaaatatga ggcatcggtg
    14521 gcggatatgg agtcggccaa ggccgagttg gaaaaaagcc ggatgcatca ggaagccctt
    14581 gaggaacaaa tacgcaattt aaaggggttg aacgaaaaag tttccacgga tttggagacg
    14641 atgaccaccg aggtgcaacg gattaaagaa gggcgaaaaa atgagcacac cttattggaa
    14701 acacgtgagc gggaattgga tcaggagaaa gagcaactca cgtccaaatt gcgtgtggtc
    14761 gtggaccaat atcaaaaagt aaaagcgcaa aacaaagccc tgaaagagac agtgttgcgc
    14821 tatcagaagg aattaaaaga tacccgtgaa tcttccatag ggggtggtgc cggagcgatg
    14881 aagtcctcgg agaagcctga aatgtccaag accgaaagtg ccccgtcagt gaattccagc
    14941 gcgatcattt cgtccaaacc acctgtgccc aaaattgtga cgcccccaac cctgcccaaa
    15001 atcggtgggg atttagtcaa tattaataag gcgccagtga gtgatctggt gttgacgctg
    15061 gggttaactc gggaggtagc tgaggaagtg gtttcaaacc ggccttatcg gctccgtggc
    15121 gaacttgtgg cgaaaaacat tattcccaag gccacatttg atgagatcaa ggaccgtatc
    15181 acggcttctc cgtaatggga atggtgaaaa atgccgccag cggggttccc tgccttcgcc
    15241 ggagcggctt cgcgcaggca ggtcgctgcc tggccttgct cacgtactcc tcgtacgctc
    15301 cgctcgtcca agcagctgag gccttccgtt cggcatgact cagggcagga ctggacgagc
    15361 agttttgact actcccagca ttcctcaaca aggggaactt catcaacaga gatgaagctc
    15421 cccatttttt tcggtgtagt cttacgacct ctccgtcgtg gttgagttat ccttcagaca
    15481 gcgatcctcg atgatttgtc catcctgcag ttgaatcagc cggtcagttt gaagggagag
    15541 tttttcgttg tgggtcacaa tcacgaaggc tgttccatgc gtgtgattga gttttcgtaa
    15601 aagtgcaaat aattcgtcac cggtatgcgt gtcgagatta cctgtcggtt catccgccaa
    15661 caccaggtca gggtgccgga tcaaggctct ggctacggcg acacgttgct gctctcctcc
    15721 ggataattcc cctggtttat ggtgcaggcg gtgagagagc cccacttcag acaggagaga
    15781 tttagccgcg tccatggcct gctgtttggg aattttttgg atgagcgccg gaagataggt
    15841 gttttccaac gcggtgaatt ccggaagaag gtgatgaaat tgaaagacaa atccgatgga
    15901 ttgattccga aatcccgcta acgcggtttc gctgacccgg aacatattct ttccttcaaa
    15961 caatactttt ccacttgtcg ggcgatctaa ggttcccatg atgtggagca gggtactttt
    16021 tcctgcgccc gatgctccga caatcgacac catttccttt cgcgctaacg tcatggtaat
    16081 gccgttgagg acatgcaccg tttgcttgcc catctgaaat gaacgacaaa gattgtcgat
    16141 agacaccaat ggcacttggg gcgtgaactc tgagtcgatt gggttattca taacgaaggg
    16201 cgctgactgg gtctaatttc gccgcttgcc atgaaggata gaacgtggcg gcaaaactga
    16261 tcaggatggc cgaagtggct actaacagca catccaatgg caggatgtgc acgggaatgc
    16321 gggatatgta atagacggag gcgtcgaagg tccaatagtt ctgaatgagc cacaaaaagg
    16381 tatagccgag agggacccca atgcccgttc ccacgattcc gattaccaat ccgtttaaca
    16441 tgaaaatccg catgatggct cctggcgtgg cgcccatggc tttgaggatg gcaatttccc
    16501 gttgcttttc gttgacaatc atcgtcaagg tccccacgat attgaacgag gcgaccaggg
    16561 tgatcaagac cagcagaaga aacatcatgg tcttttcgag tttcaatgcc gaaaacaaat
    16621 ttcgatttaa ctgcatccaa tcacgggcga taaagatgtg accgagtgtg gcttctaatc
    16681 gttgggcaat ctgctgggcc aaaaatacgt cgttgacttt gacttgaatc ccggtgaccg
    16741 tctcaccgag attgaagaat ttctgggctt cttccaggct gatatatgcc aacgaagaat
    16801 catattcgaa cataccggat tcgaatacgc cgaccacggt gtagggtcga attttcggtg
    16861 tcatggtcaa cgaggtcatt ggaccgacag gtgagacgac gttgatggta tcgccgataa
    16921 acacgccaag ccgaaaggcc aattccttcc ccaaaataat tccgggtgga ttggtgggtg
    16981 aaggagtggg attgtcccga acggaatcag gagatgtcga tgtggatggg gtaaaagggc
    17041 gagtcaggcc agccaaatcc ccaaatttca tgttggcttc taaatcggtc acgcgtgttt
    17101 cctgcgaggg ctgaatgccc cggaggatga tcccttgcac ggctgttttg gaggtgacga
    17161 gcacctgttt gaaaatgaac ggggcagcgg ctatcacatc gggaacggct tcaatctgat
    17221 cgaccaggtc cgcatagcct ttcatgtcat tgctgccacg ttcctggacg atcacatgcg
    17281 cagtggtgcc aaggattttg gactggagat cctccttgaa gccggtcatg atgccaagcg
    17341 tgccaatgag tgccgcgaca ccaagcgtga tcccggtgat ggagataaac gtgttcagcg
    17401 aaatcgttcg ctgccgacgc ttggcccgaa gataccgcag tccgactaat atctcatatg
    17461 ggagggtcac aaaggccgat ccggtcgaag ttggggaaaa agaatgacat cgcgaatcga
    17521 ggcctgattg gtgagtagca tgacgagtcg atcaatccca ataccttctc ctgctgtggg
    17581 aggcatgcca tattcgaggg ctcgaataaa gtcctcatcc agataatggg cttcgtcgtc
    17641 tcccgcatcc cgcaacgcca cctgggcctc aaagcgttgt cgctggtcca agggatcatt
    17701 taactccgaa aatccattcg ctagttctcg tccaccaatg aagagttcaa agcggtcggt
    17761 cagggccggg ttggcttctt tttttctggc caagggggaa atttcgatag ggaaatcggt
    17821 tatgaaggtt ggttgaatca aggttggctc gactgtggct tcaaagagat tgacgaggat
    17881 gtcgacatgg ctggcttgcg gcgcacaatt aacacctaac cgctttgccg cgtccacggc
    17941 tttcttatga tccgacaaga cttcagaatc caactggttg acctctacga tcgagtccaa
    18001 atacggcacc cggcgccagg gtggggccag agaaatgctt tggccctggt acgccaccgt
    18061 tgggcttccg cacacttcca gactcaaggt gtgaaacaac tgttcggtca ggtccatcag
    18121 atcatgataa tccgcatagg cctgataaaa ttccaacatg gtaaattcag gattgtgaat
    18181 ggtggaaatg ccttcattgc ggaaattgcg gttgatttca aagacgcgcc ggaatccccc
    18241 gacaatgagg cgcttgaggt ataactccgg tgccacgcgc agatataagt ctacgtcgag
    18301 ggtgttgtga tgggtcacaa acgggcgtgc cgtggctcct ccaggaatgg aatgcatcat
    18361 cggggtttcc acttcgaggt agtctcgttc cgtgagaaac gaacgaatga tattaatgat
    18421 tcgactgcgc gtgcggaata cctggtgtac cgatggattg gcaatcaaat ctacataacg
    18481 ttgccgatac cgggtttcaa tgtcggtgag cccgtgccat ttttccggaa gaggccgtaa
    18541 ccccttggtg agaaaggtga ggcgatggac ttctactgtg agttcgtcgg ttttggtgcg
    18601 aaataacttg ccttcgactc cgatccaatc ccctaaatca aacgactgcg aaagttcgta
    18661 ggactgatcc ccaagcagat ctttcttgag gtagacctgg aggcgatggc cttcttcctg
    18721 taaggccgcg aaagcggcct ttccaaaacg gcgcatggcc acaatgcgtc cggccaccgt
    18781 gcacgaaatc ggtttgcctt caaggtcttc tttggtcgag tccgtatacg attgaagaat
    18841 ggtctcaagc gtgtgggagg tgggaaaacg tgtaccaaag ggatggagac ctccatcctg
    18901 aagcgcgtgg agtttttcca gccgttgttg ttgttgttcg ttcaattctt ccataactta
    18961 catgactcta ctggattcat aggggtgaat atctcgatag tcgtctggtc agcacatgaa
    19021 acacgaatct gtcggtgatg aatcatggag gccgagtatc ggaggtggat gcgtgttaat
    19081 tggccggcat atgtgaagcc ttcgaggcct tcatggttaa atacgcctca ataaaagggt
    19141 ccagcgcgcc atccatgacc gcggcgacat tgcctgactc gtagttggtc cggtgatcct
    19201 tgaccatttg atacggttgg aacacatacg aacggatttg gctaccccag gcgatatctt
    19261 tcttttcccc aacaatattt tgaaattcag cgtctttctt ttgttgttcc agctcataca
    19321 gcttcgcttt taacacccgc atggcgccga tccggttttg caattgcgac cgttcatttt
    19381 ggcattgcac cacagttccc gtgggaagat gggtcatgcg aatagcggtt tccaccttgt
    19441 tcacattttg cccgcccgcg cctcccgcgc gaaaggtatc gactttcaga tccttatcat
    19501 caatcacaat gtcaatgtcg tcgtccaatt ccggatagac ccccactgcg gcgaatgagg
    19561 tatgccgacg cttattggcg tcaaaggggg agatgcggac cagccggtgc acgcccgcct
    19621 ctgagtgcag gtatccatag gccaatggcc cggcaatggc caaggtcacg cttttgatgc
    19681 cggcttcatc gccgggttgc agatcgatgg tttccacttt gtacccccgc tcctgagccc
    19741 atcgaatata catgcgcatg agcatctgtg cccagtcctg ggattcggtt ccgcctgcgc
    19801 caggattgat gctgagaatg gcattattct ggtcatgttc gcccgataac aattgttcca
    19861 atcgccaggc ttccagcgtg gcctcaatgt ctttgatacc tttgctccac tcggcttcca
    19921 attccggatc cccctgttgg ccaatcagat caagaatcgc gagaaggtca tcgcgttgct
    19981 ggtcgagttg gtgaaggcgt gcgagttggc gttcgatctc ggcttgacgt cgtccggctt
    20041 tggcgccctg acggggattg ttccagaaat cgggttggga ggtctcccgc tcgagttctt
    20101 caagttcagt tgttagccga ggaacgtcaa agatacctcc ggaattccag aatgtggtcg
    20161 ttgatttttt ccattcgaat gcgaatatct tcgagcatgg gcagctctcc tctcgtggct
    20221 atatcggtaa agaccgtggc ttgggcgtgg aaagcggtgt ggtaaaccga caccaagtga
    20281 acaaaatacc ggctattata acacaacccc aactaaagac atccccaaat tgagtgtaaa
    20341 aggtgggtga tgaggaacgg agtggaagta atcccgttac cgcctgttcg gagaaaattg
    20401 atgtctggga aagaatgtgt ccgtccgggg caatgaagcc ggaaatgccg gtgttggcgg
    20461 ctcgggcaaa ccctaaatga ttttcaacgg cacgaaaaac gaccatgccg aaatgttgat
    20521 acggcgcagc agaatccccg aaccaggcat cattagtaat cgtgacgaga aaattagcac
    20581 cctcccgggc catccggcga accagatccg ggaaaatcac ttcgaaacaa atcgccaccc
    20641 cgaatctcgg accactgccg ggtggcaccg ttgggacttg catggtcatg actccctggc
    20701 cggatttgaa gtcgccgatc cctaccacga gcttatccag aaaaaagaga attgatcgca
    20761 agggaatgta ttccccaaag ggcaccagat gccgtttgtc ataccggtct gaggcccgtc
    20821 cttcagccgt caccagaaag gcactattgt acaaataagg acggccgtcc tgttgaaatc
    20881 gaagggtggg actgccaaat agcaacgagt tgtgcgtgtc tttcgcaata tcaagcacct
    20941 gtttctggta ggcgggttcc tgctcgaaga gaaagggcgt ggccgcctcc ggccaaatga
    21001 gcaggtcggt attcctcccg gcttcttggc tgagcgaggc gtagcgtcga agtgtttcat
    21061 cccggtgggc ctcgttccat ttgacggctt gatcaatatt ggcttggact aa
//
LOCUS       DATIAL010000279         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1342427, whole genome shotgun sequence.
ACCESSION   DATIAL010000279 DATIAL010000000
VERSION     DATIAL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342427"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..769
                     /locus_tag="VLA60_08625"
     CDS             242..769
                     /locus_tag="VLA60_08625"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HSF09466.1"
                     /translation="MGSSLLFSGLALGLVVAAVTDVRDSRIPNWLTFSLAAFGFGVQS
                     WEHGWDGFLFSLGGLGVGLACLMFFYIKGGMGAGDVKLLGAIGTILGPGHVVIAFAFA
                     AMLGGLYSLALLSNQGGMGHAWNRVFLLLSTLKVTRTLPVSGEQIPADPKLRYALVLG
                     LGTVIAQTLFYYGVL"
     gene            956..1801
                     /gene="cpaB"
                     /locus_tag="VLA60_08630"
     CDS             956..1801
                     /gene="cpaB"
                     /locus_tag="VLA60_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSF09467.1"
                     /translation="MGQKRPLVFLAIAMGIALVTTVMVYRWLQGQRMIEVDTPEVMVE
                     GVSVAVASADIPWGTPLAEKTVRTIMYPEEGVPAGHFKDPASLKGRVVLTNLKKNEPI
                     LESKLAPIDIKTGGVSAVMDPTKRAMAVKVNEIVGLPGFVQPGDRVDVMATFEDPRGK
                     KKENGGARVEVTKVVLENTLVLATDTQMERANGEEKPIPVKVITLEVSLEEAEKLAMA
                     ENGGKLRLALRSPLNPEVKKTKGADFRDLMSSHQIIPPPAKAAHKPENQVEVIKGTDR
                     KTMKF"
     gene            1848..>2095
                     /locus_tag="VLA60_08635"
     CDS             1848..>2095
                     /locus_tag="VLA60_08635"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HSF09468.1"
                     /translation="MNQTHGFPITSMLMALTLVLASYTGALGEGLTTQAVRVNEPQRL
                     GLTVGESRIVETKMSFKRASVANPDIADQIVLSPKQIYL"
BASE COUNT          528 a          450 c          592 g          525 t
ORIGIN      
        1 gggaggggag taaatgctgg ttggataagc aagaggagat ttaacggaca tcagttatcc
       61 gcttccaaag ctaggcctta agaaagttcg gcaactcacc gaaactaatt tttgaatgca
      121 gtgctgttgc tttattactg atagtggccg tgctcatccg gcagagatta ttcccgagtc
      181 accttctcat agaattcacc ttagattttg aaaaatacaa tcgacggatc atcctgaaat
      241 tatgggttcg tctctcttgt tttccggatt agccttagga ttagtggtgg ctgccgtcac
      301 cgatgttcgg gacagccgga ttcccaattg gctgactttt tctctcgcag cctttggatt
      361 cggcgttcag agctgggagc atggctggga cggattcctg ttcagtctcg gcggattggg
      421 tgtggggttg gcctgtttga tgttttttta tattaaaggg ggaatgggag cgggagacgt
      481 gaaattgctc ggagccatcg gaacgatcct gggacctggc cacgtggtta ttgcgtttgc
      541 gtttgcagcc atgttgggag ggctgtatag cctggccctg ttgtccaatc aaggtggcat
      601 ggggcacgcg tggaatcgag tgtttcttct cctttcgact ttgaaagtaa ctcgtaccct
      661 ccccgtgtcc ggagagcaaa tcccggcaga tcccaagttg cgatatgccc tggtcttggg
      721 gttgggaacc gtcattgcac aaaccttatt ttattatggt gtgttgtaac cgcggtgttg
      781 ggctttgttg tgggtggaga ttaaatgatc ggacacgatt cttttcgttg actataaaga
      841 atgacatttt ccatccgata ttccgatata cagagtcaat gggttgattc atcaaattta
      901 taggcaagaa cacattcggc ggtctcgatt cggcgaataa gggggaggaa gcctcatggg
      961 acaaaaacgt ccactcgtgt ttttagcgat tgcaatggga atcgctcttg tgacgacggt
     1021 tatggtgtac cgatggttgc aagggcaacg aatgatcgaa gttgacacac ccgaagtcat
     1081 ggttgagggt gtgagtgttg ccgtggctag tgctgatatc ccgtggggaa ctccactcgc
     1141 tgaaaaaact gttcggacca taatgtatcc cgaagaaggg gtgcctgcgg ggcattttaa
     1201 agatccggct tccttgaaag gccgggtggt gttgacgaac ctcaaaaaaa atgaacccat
     1261 tttagaatct aaactggccc ccatcgatat taagacggga ggcgtgtccg ccgtcatgga
     1321 ccccactaaa cgagcgatgg ccgtgaaagt caacgaaatt gtcgggcttc cggggtttgt
     1381 acagcctggc gatcgagtcg atgtgatggc gacatttgag gacccaagag gcaagaagaa
     1441 agagaatggc ggtgcgagag tagaagtgac caaagtggta ctcgaaaata ccctcgttct
     1501 ggcgaccgat acgcaaatgg agagagcaaa cggagaggaa aaacccatac ccgtgaaagt
     1561 cattacgctg gaagtgtcct tggaagaagc cgagaaattg gccatggccg aaaacggggg
     1621 aaaattacgt ttggctttgc ggagtccact caatcccgag gtgaagaaaa ccaagggagc
     1681 ggactttaga gatctgatga gttctcatca aattatacct cccccggcca aagcggctca
     1741 taagccggag aatcaagtcg aagtgatcaa agggaccgat cgtaaaacca tgaaatttta
     1801 agagggtcgt cgatggggat gcgatgatcg ttatgaaagg tatcgatatg aatcagactc
     1861 acggttttcc aatcacgagc atgttgatgg ctctgaccct ggttctggct agctatacgg
     1921 gagccttggg agagggtctc acgacccagg cagtccgagt caacgagccc caaagactcg
     1981 gtctaaccgt tggtgaatcc agaatcgtgg aaacgaagat gtccttcaag cgggcttcgg
     2041 tcgccaatcc cgatattgcg gaccaaattg ttctctcccc caaacagatt tatct
//
LOCUS       DATIAL010000280         7989 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_52186, whole genome shotgun sequence.
ACCESSION   DATIAL010000280 DATIAL010000000
VERSION     DATIAL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52186"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1680
                     /locus_tag="VLA60_08640"
     CDS             385..1680
                     /locus_tag="VLA60_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSF09469.1"
                     /translation="MKNSKLNNFVPFSLRFRKQGGQQSPTHLREFGWPFVLLCVGLVV
                     VGLLGLSGKPEVLPEQAESDLEVFVRQGCPHCEKAKSYLTRLKQQYPKFTVTIRDIGE
                     DPQALLRLKALAAKLGITQLGVPAFYGRGELLIGFESAETTGQQLEELLGRPPPDAGT
                     SSEGACPVEPDMPCPPVSTQSDAGEKSVQVPFLGDRTLPELGLPLFTLFLGLLDGFNP
                     CAMWVLLFLLALLANLRDRRKMFLLAGTFVLVSGVVYFAFMAAWLNVFLFIGYVRIIQ
                     VLMGGLAVGIGLLNVKEFWAFGQGLSLTIPESVKPGLYARIRKILAAEHVGQAMIGIL
                     VLATLVNMVEFVCTAGFPAMFTQVLSQQGLTTFEYYGYLGLYNLAYVADDAVMVTIAV
                     VTLSHRKLQAREGRWLKLISGLVMLGLGLLLLFAPTQLF"
     gene            1850..3586
                     /locus_tag="VLA60_08645"
     CDS             1850..3586
                     /locus_tag="VLA60_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF09470.1"
                     /translation="MLAKHEHDLLYGQQIEQLYALAPVGIIASLVNGSILTVIQWNVI
                     SHGLLLTWFAGLFLLNGAWTLLWYQFSNASRHPQDSHRWGRRFLGGTLASGILWGATG
                     IVLFPVSSIPHQIFLAFVVGGMIAGATAVYAPLQGAFYAYVLPAMTPLIVQFFLLEDE
                     RHMAMGGMCLLFVTMMVVTLRHNHSITMASMTLNLELGKSNQALQREIGQREQAEVAL
                     RESREQLHSIVQSTDEGIISLNSQGKVMLWNTGAEVLFGFTMEEMKGQTLECIIPERF
                     RQAHQQGILRASRAGRKTVVGEMFELMGLRRDGSEFPLELSLGYWHKHGEIFFTGIVR
                     DITARKKTERALHCRERELEQSQEELRALGAQLISAQEDERRRLSRELHDDMNQRLAM
                     VALEIDSVQRSLPESDPMQKTLQHLNDQVSALSDSVLHLAYQLHPSILDDLGLVVALK
                     SSIKEFSQWENIAVTFQPRDVPQFLPQDIASCVYRVTQECLRNVAKHAQASQVSVEVR
                     GVEGGLQLVITDNGKGFIPESGLRGTRGLGLIGMKERIRVVQGTFEVKASEGKGTTVT
                     AWIPLSRLVNSD"
     gene            4098..>7989
                     /locus_tag="VLA60_08650"
     CDS             4098..>7989
                     /locus_tag="VLA60_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HSF09471.1"
                     /translation="MSYIGVNIGALTVKVAALRGDTRDAKVVAHQGRPLQVLKELLAG
                     PEFADAEYYGVSGQLGHIPEVAAIQRALGEVEGQFDAVVCLGGESFLVYLLVDGKITN
                     VLSHNKCAAGSGEFFMQQIGRMGLETEEAIQRSFSGKVVPLASRCSVHCKSDITHKLN
                     RNEATAEDILHTLHDSMSNKVIALLEKGMHEPKRVLLTGGVTRNAAMLAALREKLPAT
                     DFVVLPESPWFEAWGSALIARDEPVYTSPHISAQASLGHLPPLHRYADRVKIIPALPH
                     QAPPDGMMVLGVDAGSTTTKAILLDPATYGVVASHYARTQGDPVRATRECLHALVDQL
                     GNRAVGLVGTTGSARELVGAYLGTEYVYNEISAHAAGANHFDTGVDTIFEIGGQDSKY
                     IYLRNGVPIDYAMNNACSAGTGSFLEESAHGDLGMNVSEISNIALDAPSPVHFKATCA
                     AFINSDIRIAQQGGHPRDNIVAGLVYAIVSNYLNRVKGPRYVGKKVFLQGGVALNHAI
                     GHAFAHSIGRDIVIPPSPELLGALGVGLLALQRSGGTLGTATNLLALAEPEMTLAGRF
                     TCGACAMYCSIDRFIVAGRRFPFGGRCSLFESVWKRKVRTVAAPDLVEKRAKLIFTSA
                     PPPMSTTPNQEGGNHIGWGVKRAFDSAKSLVEASLGHPHPVTAGVNETKQQIGIPRAL
                     MTHSLYPLYSTFFSSLGMEVVLSDVDPRGDLKSNAGFCFPIQIAHGAVLDLSRKKLDL
                     VFLPQVTRMPHQNACRECYLCPITQACPYFMSKAFSDIRFLSPVLDFVNGYEGCVALV
                     DMAVEDLQMPRELAQQAWEAAIRVQRESEQALRDLGQSALNEAVASGKPAILLAGHSY
                     SAFTQEASQSVGKKLSSMGVPVIPADCLASIEDGSTSWHFANQILNAMAVTKKYPNLF
                     LLCVSNFSCTIDAFTHSVLSSEMGAKPYLILEIDAHTADAGVQTRLEAFLDIVQNYRE
                     AQTSPGKPFIPCRLTSSGHVARSNGEQVRLDDPRVKIYFPNFSDYHTQAAAMSAQWLG
                     LHVGNLIPLDRSQLDRGLQHTSGRECLPLPICIGQLLQVDEHRRPGEIAGFYMIRGGA
                     PCVTDCYMGYFERFIAEQRLSDIFLLSPGPENNYGGLDLASLPKHLLPTMHVADILVE
                     IEQVLRVVGTPESVQELKGEWQRFRHAARSFDQFQSGLPGLIDRLAAFPRERDPLTCP
                     RVVVTGDFFTRFSPFFMDGVRDRYNQKGIILKPVDLGDLVLYDSYDRITRNADGWGMK
                     PGGLAMAKACTRILQPDGREYLQQWLGHQTVEWSE"
BASE COUNT         1683 a         1992 c         2265 g         2049 t
ORIGIN      
        1 gaccttcacc gtatattcca cgcgagcgat tttcatcctg gtcgtgttct cctcttttcg
       61 ttgtctttca tcctgactct cacgaagccg ctgtagggta ttgcgacagg tgaatctggg
      121 acgggtcata cttgatctat aggccggaag atggcaaatt tttaggccat gtatgcggtc
      181 ttagtgccag agccggatta ttggtacggt caattctagt tgttgcaggg cctaaaggcc
      241 acctaggaat tgaagatgtg tcacctcaaa ccttttctgt gctagattta ttttttcaat
      301 aatttattga ctagggatca accttttccc aagctgcgcg aaatatatcg cattccttct
      361 tgacctttcg agcatatccc atagatgaaa aactccaaac tcaataattt tgttcccttt
      421 tcccttcgct tccgaaagca aggtgggcag cagagtccaa ctcatttgag ggaatttgga
      481 tggcctttcg ttctgttgtg cgtgggtctt gtggtggtgg ggctgcttgg tctgtcaggg
      541 aagcctgagg ttcttccgga gcaggctgag agtgaccttg aagtgtttgt ccgccaggga
      601 tgcccacatt gcgaaaaggc caaaagctat ctaacccgac tgaagcaaca atatcccaaa
      661 ttcaccgtca cgattcggga tattggggaa gatcctcagg cgttacttcg attgaaagca
      721 ttggcggcga aattgggtat tacacaactg ggtgtgccgg ctttctatgg gcgaggggag
      781 ctgttgatcg ggtttgaatc tgccgagaca accgggcagc aactggaaga attgttaggg
      841 cgtcccccac cggatgcggg aacttcctct gaaggtgcct gtccggttga acctgatatg
      901 ccctgtcccc cggtatccac gcaaagcgat gccggggaaa agagtgttca ggttcctttt
      961 ttgggtgacc ggactcttcc agaactcggc ctaccgcttt ttacactctt tctcggactt
     1021 cttgatggat tcaatccttg tgcgatgtgg gtcttgctgt ttcttctggc gttgctggcc
     1081 aaccttcggg atcggcgcaa gatgtttctc ttggcgggca cattcgtcct ggtcagcgga
     1141 gtggtgtatt ttgcgtttat ggcggcgtgg ctgaatgtct ttctgttcat cgggtatgtg
     1201 cgaatcatcc aagtgctgat gggtgggttg gccgtcggca tcggcctgct gaatgtcaag
     1261 gaattttggg cctttgggca aggcctttca cttaccattc ccgagtcggt gaagcctggg
     1321 ctctatgccc gaattcgaaa aattttagcg gccgagcatg ttggtcaggc gatgatcggc
     1381 attctggtgt tggccaccct agtgaacatg gttgagtttg tgtgtacggc gggctttccg
     1441 gccatgttca ctcaggtgtt gagccaacag ggtctgacca cgtttgagta ttacggatat
     1501 ctcggtctct acaatctggc ctatgtcgcc gatgatgcgg tgatggtgac gatcgccgtg
     1561 gttaccctaa gccatcgtaa attgcaagca cgggagggcc gatggctgaa gttgatcagc
     1621 gggttggtca tgctgggcct gggtttactt ttgctgttcg ctccaacgca attattctaa
     1681 ccgcaatctg tctgaggcct tgaattgaag gatccttgtg attattgaat aagagtttta
     1741 aagtaacgta gttctaaaag atacacagaa ttggtcttgc gaatgtccgt atgacgttgg
     1801 aaacagaaga aggtctcaga acgttccctg gagagtttgt ggagggtccg tgttggctaa
     1861 acacgaacac gatctcttgt atgggcagca aatagagcaa ctctatgccc ttgcaccggt
     1921 agggattatt gcctctctgg ttaatggatc cattctgacg gtaattcaat ggaacgtgat
     1981 ctcccatggc ctgttactga cctggtttgc cggcctgttc ctgctcaacg gggcatggac
     2041 cctgctttgg tatcaattta gcaacgcatc tcgacatccg caggattccc atcgctgggg
     2101 gcgtagattt ctaggaggga cccttgcctc cggcattctt tggggggcaa ccggtatcgt
     2161 actctttccc gtaagctcca ttccgcatca gatttttttg gcctttgtgg taggaggcat
     2221 gattgccggg gcgaccgcag tctatgcgcc cttgcaaggt gctttttatg cctatgttct
     2281 tccggccatg accccgctca tcgttcaatt ctttctcctg gaggatgaac ggcatatggc
     2341 catgggaggc atgtgtctgc ttttcgtgac aatgatggtt gtcaccttac gacacaacca
     2401 ttccatcacg atggcctcaa tgaccttaaa tcttgaattg ggcaagtcca accaagcctt
     2461 acaacgcgaa atcggtcagc gtgaacaggc agaggtggct cttcgggaaa gccgggaaca
     2521 gttacattcg attgtgcaat caaccgacga ggggatcata tcgttgaata gtcaggggaa
     2581 ggtcatgttg tggaacacag gagcggaagt cctgtttggc tttacgatgg aggagatgaa
     2641 aggtcagacc ttagagtgca ttattcccga acggtttcgc caggctcatc aacagggaat
     2701 cttgcgggcc tcgcgagcgg ggagaaagac cgttgtggga gagatgtttg agctgatggg
     2761 acttcgacgg gacggcagcg aattccctct tgaactgtct ctgggatatt ggcacaagca
     2821 cggggagatc ttttttaccg gcattgtccg ggatatcaca gcacgcaaga agacagaacg
     2881 ggctcttcat tgcagagagc gggaacttga gcagagccag gaagagctcc gggctcttgg
     2941 tgcacaactc atttctgctc aagaagatga gcgccgccgt ctctcccgtg aactgcacga
     3001 tgatatgaat caacggttag ccatggtcgc gctcgagata gattctgttc agaggtctct
     3061 gccggagtcg gatccgatgc aaaagactct tcagcacctg aatgatcaag tgtctgcgct
     3121 ttccgatagc gtcctgcatc tggcctatca gcttcatcct tcgattttgg atgacctggg
     3181 gttagtcgtc gcccttaaat cctctattaa agaattttct caatgggaga atattgcggt
     3241 cacgtttcaa ccccgggatg tccctcagtt cttgcctcag gatattgcgt cgtgtgtgta
     3301 tcgggtgacg caggaatgtt tacgaaatgt ggcgaagcat gcgcaggcgt ctcaagtgtc
     3361 ggtggaggtg agaggggtag agggtggcct ccaactcgtc attacggata acggtaaagg
     3421 attcatccct gagtctggtt tgcgaggcac tcgcgggtta gggctgattg gcatgaaaga
     3481 acgaattcgt gtggtgcaag gcacatttga ggttaaagcc tcagagggca aaggcactac
     3541 cgttactgcc tggattccac tctcccgact agttaattcc gattgagaaa tttggtcttt
     3601 cctttcaaca aacctttccc tccattcaag cctatctgtt tggccagcgg cacagtccta
     3661 agccgtcagg ggtaaacttt tctgctaaaa acctttgatg gtctgtcgca tttcactaca
     3721 gggcgattta tcttctgccc ccaaacgcgt cacagcagta tgagtgggtc tcacgtcctc
     3781 ttaaatctcc cttgtttctc cccatcttct ctagagatgt cttttgacct ttctggctcc
     3841 cttcctgcac ctctctgaag gtaatgtgtc gtgagccctc tgtgaagcaa ggttctcaga
     3901 gggtgcggta taactgatat cgagcacttc aatgggagat gaagagttca gcaagagaag
     3961 cgttcatttt gttgattggc catcataatg tcattcaaga gaaaaagcct gttcggggaa
     4021 agagcgaatg atgagtcttt caagcccagt ggcgagctga aggatgtacc cactctctgg
     4081 tttcgggagg tttgtccatg tcttatattg gtgtgaacat cggtgctttg accgtgaagg
     4141 tggcagcgct ccggggcgat acgagggatg ccaaggtggt ggctcatcaa gggcgtccgc
     4201 tgcaggtgct aaaggaattg ctcgccggcc cggagttcgc agatgcggaa tactatggcg
     4261 tctccggtca gcttgggcat attcccgaag tggccgctat tcagcgagcg cttggtgagg
     4321 tagaaggcca gtttgacgcg gtggtctgtc ttgggggcga atccttcctg gtctatctcc
     4381 tcgtggacgg aaagatcacc aatgtcttat ctcataataa atgtgcggcc ggaagcgggg
     4441 aattttttat gcagcagatc ggccggatgg ggttggaaac ggaagaggca atccagcgtt
     4501 ctttcagcgg gaaagttgtg cccctggcct cccgctgctc tgtgcactgc aaatcggata
     4561 tcacccataa gctgaatcgg aacgaggcca ccgccgaaga tattttacat acgcttcatg
     4621 acagtatgtc caacaaggtg attgcgttgt tggagaaagg aatgcacgag ccgaaacggg
     4681 tgctcttgac cggcggggtg acccgtaatg cggccatgct ggcggcgttg cgcgaaaagc
     4741 ttccggccac ggattttgtc gtgctgccgg agagtccctg gttcgaagcc tggggcagtg
     4801 cattgatcgc ccgggatgag cccgtttaca cctcgcctca tatctcggct caggccagcc
     4861 ttggccatct cccgccgctt catcgctatg ccgatcgcgt aaaaattatt ccggccttgc
     4921 cacatcaagc gccccccgac ggcatgatgg tccttggagt cgatgccgga tcgaccacga
     4981 ccaaggcgat tctactggat cccgctacgt atggggtggt ggcttcccat tatgcgcgca
     5041 cacagggaga tcctgtgcgg gccactcgtg aatgccttca cgccctggtg gatcaacttg
     5101 gcaatcgtgc cgtgggtctg gtgggaacga ccggttcggc acgagagctt gtcggggcct
     5161 atttggggac cgaatatgtc tacaacgaaa tctcggctca cgctgcggga gcgaatcatt
     5221 tcgataccgg ggtggacacg atttttgaaa ttggtgggca ggactccaaa tatatttatc
     5281 tgcgcaacgg ggttcccatc gattatgcca tgaacaacgc atgttcggcg ggaaccggct
     5341 cgttccttga ggagagtgct cacggagatc tgggcatgaa tgtgtcggaa atttcgaaca
     5401 tcgccctgga cgcgccttcg ccggtccatt tcaaggcgac ttgcgccgcc ttcatcaact
     5461 ctgatattcg catcgcgcaa cagggggggc acccacgtga caacattgtt gccggattgg
     5521 tctatgccat cgtcagtaat tatctcaata gagtgaaggg gccgcgctat gtgggaaaga
     5581 aagtgtttct gcagggcgga gtcgcgctga accacgcgat tggccacgcc ttcgcccata
     5641 gtatcggacg cgatatcgtc attcccccca gccctgagtt actgggtgcc ctgggggtcg
     5701 ggttactcgc acttcagagg tccggtggca cactcggcac ggcaaccaat ctgctggcct
     5761 tggccgaacc agaaatgacg ctggccgggc ggtttacctg cggggcgtgc gccatgtatt
     5821 gcagtatcga ccggtttatc gtcgccggcc ggcggtttcc cttcggcggt cgttgcagcc
     5881 tttttgaaag cgtgtggaaa agaaaggtcc gaaccgttgc ggctcccgac ctggttgaga
     5941 agcgggccaa attaattttc accagtgccc caccccctat gtccaccact cccaaccagg
     6001 aaggagggaa tcacattggc tggggggtca agcgtgcctt cgatagcgcg aagtcacttg
     6061 tggaagcctc cctcggccac ccccaccccg tgaccgccgg tgtgaacgaa acgaagcaac
     6121 agatcgggat cccacgagca ctcatgacgc attccctcta tccgctctat tcaacctttt
     6181 tctccagcct ggggatggag gtggtgttat cggatgtgga cccgcgtggc gatctgaagt
     6241 cgaacgcggg cttctgcttt cccatacaaa ttgcccacgg cgcggtgtta gatctctccc
     6301 gaaagaagtt ggatctggtt ttcctgcctc aggtgacgcg catgcctcat caaaatgcct
     6361 gccgggaatg ctatttgtgt ccgatcaccc aagcctgtcc gtattttatg tctaaagcct
     6421 tctccgatat tcgctttctt tcgccggtgt tagattttgt gaatggctat gaggggtgtg
     6481 tggcccttgt ggatatggct gtcgaggatt tgcagatgcc gcgcgagttg gcacagcagg
     6541 catgggaagc ggctatccgg gttcagagag aaagcgagca ggcccttcgg gaccttggtc
     6601 agtcggcctt gaacgaggcg gtggcctcgg gaaaacccgc gattttactc gccgggcaca
     6661 gctacagtgc atttacccag gaggcgtctc aatcagtggg aaagaaatta tccagtatgg
     6721 gggttcccgt gattccagcc gattgtctcg cgtctatcga agacggatcc acgtcatggc
     6781 attttgctaa ccagatttta aatgcgatgg ccgtgaccaa aaaatatccg aacctgtttt
     6841 tgctctgtgt gagtaatttt agttgcacca tcgatgcctt cacgcattcc gtgctgtctt
     6901 ccgagatggg cgccaagccc tatctgattc ttgaaatcga tgcgcacacg gctgatgcgg
     6961 gcgtgcagac acggctggag gccttcctgg atatcgtcca gaactaccgt gaagcgcaga
     7021 ccagccccgg caaaccgttt atcccatgcc ggttgacgtc cagcgggcac gttgcacgct
     7081 cgaacgggga gcaggtgcgg ttagacgatc cacgggtaaa aatttatttt ccgaatttct
     7141 ccgactacca tacgcaggcg gcggccatgt cggcccagtg gttagggttg catgtcggca
     7201 accttattcc attggatcga agccagcttg accggggcct tcaacatacc tcgggacgtg
     7261 agtgtcttcc cctgcccatt tgtattgggc agctgctcca ggtggatgaa caccgtcgtc
     7321 ccggtgaaat tgccggcttc tatatgatcc gtggtggtgc cccttgcgtg accgactgtt
     7381 acatggggta ttttgagagg tttattgccg aacaacgact gtccgacatt tttctcttga
     7441 gtcccggacc ggaaaataat tacggaggat tagatttggc atccctgccg aaacatctat
     7501 tgccgacgat gcatgtggca gacatcctcg tggaaatcga gcaggtactg cgggtggtgg
     7561 gtacacctga aagcgtgcaa gagctcaaag gcgagtggca gcgattcagg cacgcggctc
     7621 gctcattcga tcaatttcaa tccggactcc ctggattgat cgatcgcctt gccgcctttc
     7681 cacgtgaacg ggatcccttg acctgtccgc gagtggtggt cacgggcgat ttttttaccc
     7741 ggttcagtcc tttttttatg gacggtgtgc gtgaccggta taaccaaaaa ggcattattc
     7801 tcaagccggt tgatctgggt gaccttgtgc tgtatgactc ctatgaccgg atcacaagaa
     7861 atgccgacgg gtgggggatg aaaccaggag gtttggccat ggcgaaggcg tgcacaagaa
     7921 tccttcagcc ggatggcagg gagtatctgc agcagtggtt gggacaccag accgtagaat
     7981 ggtccgagc
//
LOCUS       DATIAL010000281         5189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_375750, whole genome shotgun sequence.
ACCESSION   DATIAL010000281 DATIAL010000000
VERSION     DATIAL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375750"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VLA60_08655"
     CDS             <1..263
                     /locus_tag="VLA60_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HSF09472.1"
                     /translation="IAEIITETAAIQKTDSMEMVEHVLDLIKSMLQQGEVVKIAGFGN
                     FVVRNKGERKGRNPRTGEEIAITPRRVVTFRPSQLFKKYVNS"
     gene            331..681
                     /locus_tag="VLA60_08660"
     CDS             331..681
                     /locus_tag="VLA60_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HSF09473.1"
                     /translation="MGVGHKYEDKAFYKIGEVADLTNLPAYVLRFWESEFIFLKPKKS
                     QGKHRVYSKADIETVFEIKRMLYDEVYTIAGLKRYWYRKKRSEKAVQVPKDRVEKAKV
                     ELQGILKILGRTTV"
     gene            731..807
                     /locus_tag="VLA60_08665"
     tRNA            731..807
                     /locus_tag="VLA60_08665"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:765..767,aa:Pro,seq:ggg)
     gene            complement(890..1144)
                     /locus_tag="VLA60_08670"
     CDS             complement(890..1144)
                     /locus_tag="VLA60_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09474.1"
                     /translation="MQEDILQAYLEIEQAMQRYSMLLQEHVSRLETQTDPESKNRFHR
                     MKAGSKAMRDSSQIYLSYAKYVAYGMPEGEELAEEEDLQA"
     gene            1383..2141
                     /locus_tag="VLA60_08675"
     CDS             1383..2141
                     /locus_tag="VLA60_08675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF09475.1"
                     /translation="MSLQPPRHWISVVIPIKDERENIPLLASQLLKFFESRPESGSAA
                     FELVFVDDGSVDGSGPLLDELSKQFPALRVIHLDRNHGQTAAFDAGFREARGELVATM
                     DGDLQYDPNDFAKLLPFVERYDLVCGRRQTRHDNLIRRWSSKIANQVRNWAVHDGISD
                     TGCSLKVFKQAVVKRLPPFKNMHRFFPALAKMYGFTVTEIPVQHFPRAHGTSKYGIGN
                     RLFAGLYDLFAVRWMQKRCLNYTLKGSHPEKSEP"
     gene            2250..2564
                     /locus_tag="VLA60_08680"
     CDS             2250..2564
                     /locus_tag="VLA60_08680"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HSF09476.1"
                     /translation="MFESFTHSLLAMSFEELLWIGFGFLGQGIFFSRWLVQWVVSERN
                     EESQIPMSFWYMSLVGSVIVLTYAIHIADLVFMAGQSVGTVVYVRNIMLLHQANKRVM
                     SG"
     gene            2641..4308
                     /locus_tag="VLA60_08685"
     CDS             2641..4308
                     /locus_tag="VLA60_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HSF09477.1"
                     /translation="MLLLAAWLLGVDGGTLFGPDLGALGLTDRDEGSNAEAAREMLES
                     GDWISPTLNYEPRYAKPAFVYWLISGSYSLFGVNEFAARLPSALSGLCLLLLQYVFVR
                     RWAGPTVALFASCMLLLNLEFLGINRMVLTDPELVVFTTLAGYSFWHALHHEQTKRWF
                     FAIFYLAMGCGMLVKGPVGIIIPLVGVIPYLTLTRQWGIFWQRGFPLLGILLVAAVAA
                     PWYVMMFQIHGEAYWAAAQANTTGRFMNPMEGHGGTLLFYVPVLLVGFFPWSAFLPSV
                     LYHTFKRWKAFWQGQGPATQEQHLECFLSLWVVGLLLFFTFSATRLPHYIYPLFPAAA
                     LLVAQWWKRFLTEERPAGLTASTRVLIGVGYLLGIAMMFVPAVFQLFMKQIAKEFPAA
                     IQVDLAWLPSLVGLVLVLGTMLMRQCMRSDSKRHLAFWVGCGIMLIFTVLVARGGLSI
                     YQQYFIGPPQELAVIAGYNLGPDDRLIQFGRKRPSLSFYAKRKVYFLGVNDEELPQHL
                     SAPGRKMAIIQTQLRGQLPEAMAHWTVVLDHGGFSLLTSEPLLKDNS"
     gene            complement(4908..>5189)
                     /locus_tag="VLA60_08690"
     CDS             complement(4908..>5189)
                     /locus_tag="VLA60_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSF09478.1"
                     /translation="RQELIEAALWHESKEPVLGRRFRNEVGYVVDRFAETPFLWQERE
                     GGYRRVNCPIFPYYIPYFVRGEKIILVAVAHGHRQPGYWQSRQTFSTGL"
BASE COUNT         1144 a         1279 c         1373 g         1393 t
ORIGIN      
        1 acattgccga aatcatcaca gagacggcag ctatccaaaa aaccgactca atggaaatgg
       61 tggagcacgt cttggatttg atcaagtcga tgcttcaaca gggggaagtc gttaaaattg
      121 ccgggtttgg taactttgtt gtccggaaca agggagagcg gaaaggcagg aacccaagaa
      181 ccggagaaga aattgccatt acgcccagac gagtggtgac cttccgacca agccaactct
      241 ttaagaaata tgtcaattct tgactggcga gaatctcgca ttcggtctcg ttcactctct
      301 tgctagacac accgtggggt gattgcaggt atgggggttg gacacaaata cgaggataag
      361 gccttttata aaatagggga agtcgccgat ttgacgaatc ttcccgcgta tgtcctgcgg
      421 ttttgggagt cggaattcat ttttctcaaa cccaagaaaa gtcagggcaa acaccgggtc
      481 tattcgaaag ccgacattga aacggtcttt gaaataaaac gaatgctcta tgatgaagtg
      541 tatacgattg cgggcctgaa gcgatattgg tatcgcaaaa aacgatcaga aaaagccgta
      601 caagtcccaa aagaccgagt ggaaaaagcg aaggtggaat tgcaaggtat tctgaagatt
      661 ctggggcgaa cgacggtgta gggtttcgac caatgacgac ggatgggaat acgactggtc
      721 agaataatgt cggggcgtag cgcagcccgg tagcgcactc gcttggggtg cgagaggtcg
      781 gaggttcaaa tcctctcgcc ccgaccatgt tttcttttca gcagaaaggg tttcttcgtc
      841 tcctcggtca gttcataggg gagagtcaat tgggtagtac gaaggctcgc taggcttgta
      901 agtcctcctc ttcagccagc tcctcaccct ctggcattcc atacgccaca tatttcgcat
      961 aggagagata aatctggctg ctgtctctca tggcctttga gccagctttc attctatgaa
     1021 atcggttttt ggattccggg tcggtctgtg tttcgagccg gctcacatgt tcctgtaata
     1081 acatggaata gcgttgcatt gcttgctcaa tttccaaata ggcttgcaga atgtcttctt
     1141 gcatgaatgt tcctccttca aaatgtcctc caccttacaa aaggctgttc ccgtggtcaa
     1201 ctgaccgtga ccgtcggtgt ggagtgtagc atgacgatca cgggatctcg gggagagcct
     1261 ctcgtgatga agagtcgttt gaccgatttt ttagatattc agtaaaatgg tgaccgggta
     1321 ggagggtccg caacgtgtgt tccgacatta ttttgaaatc tgtagcgtag tccgcgccat
     1381 ccatgagtct ccagccgccg cgccattgga tttccgttgt tattcctatt aaagatgagc
     1441 gggagaacat cccgctatta gcgagccaat tgctcaagtt ttttgagtca aggcctgaaa
     1501 gtggctccgc ggcatttgaa cttgtctttg tggacgatgg aagtgtggac gggagtgggc
     1561 ccttgctgga tgagctatcc aaacagtttc ctgcactacg ggtgattcat ttagaccgga
     1621 atcacgggca aacggcggca ttcgatgcgg gctttcggga ggccagagga gaactcgttg
     1681 ccaccatgga cggtgatctg cagtatgacc cgaatgactt tgccaaactg cttccattcg
     1741 tggaacggta tgatttagtg tgtgggcgac gacaaaccag acatgacaat ctgatccgtc
     1801 gatggtcgtc aaaaattgcc aaccaggttc gcaattgggc tgtgcatgac ggcatttcag
     1861 ataccggctg ttccttgaag gtctttaagc aggccgtggt caaacgactt cctccattta
     1921 aaaacatgca ccggtttttc ccggcgctgg cgaaaatgta tgggtttacg gtgaccgaaa
     1981 tccccgttca gcattttcca agagcccatg ggacatcaaa atacggaatt ggcaacagac
     2041 tttttgccgg tctgtatgat ctcttcgcag tcagatggat gcaaaaacgc tgtttgaact
     2101 acaccctgaa gggtagccat cccgagaaat ccgaaccgta gtattttgaa ttaaccatct
     2161 ggaaatgatt gataccacaa acctttaatg caccggaagg gatctgaatt gatagacccc
     2221 tttcggcgat tcattccctg ttactgacaa tgttcgaatc cttcacgcac tctctcctgg
     2281 ccatgtcgtt tgaggaactc ctctggattg gctttggttt tttggggcaa ggcatttttt
     2341 tctcacgctg gttggtgcaa tgggtggtgt cggaacgcaa cgaggaaagc caaattccca
     2401 tgtcgttctg gtacatgagt ctggttggga gtgtgattgt gctgacttat gccattcaca
     2461 tcgccgatct ggtattcatg gccggccaaa gcgtcggaac cgtggtgtat gtccgcaata
     2521 tcatgctgct ccatcaggcc aataagcgag tcatgagtgg atagccctca ccctcaagca
     2581 ctctttccgt cctctctccg ctcctcaata acgcaatctc ctttttttca ggtggggtgt
     2641 gtgctgctct tggctgcctg gctcttgggc gtagatggcg gaacgctctt tggtccggat
     2701 ctgggcgcgc tggggctgac ggatcgggat gaggggagta atgccgaagc ggcccgggaa
     2761 atgctggaga gcggtgattg gatttcccct actctcaact atgaacctcg ctatgccaaa
     2821 ccggcttttg tgtattggct catcagcggg tcctattccc tgtttggcgt caatgaattt
     2881 gccgcgcggt tgccttcggc tttgtccggt ctctgcctgt tgttgctcca gtatgtgttt
     2941 gtgcgaaggt gggctggccc cacggtggcc cttttcgctt cgtgcatgtt gctgctaaat
     3001 ttggagtttt tgggcatcaa tcgcatggtg ctgaccgatc cggaactggt cgtgttcacc
     3061 accttggccg gctatagttt ttggcacgcc ctccaccatg aacagaccaa gcgatggttt
     3121 ttcgctatct tttatctggc catgggatgt ggcatgttgg tcaaggggcc cgtgggcatc
     3181 atcattccgc tggtgggcgt gatcccctat ctcacactca cgcgccaatg gggaatcttc
     3241 tggcaacggg gctttcccct tctcggaatc ctcttggttg ccgctgtcgc ggctccctgg
     3301 tatgtgatga tgtttcaaat tcatggggaa gcctattggg ccgcagcgca ggccaatacc
     3361 accggccggt tcatgaatcc catggaaggc catgggggga cgctcttgtt ttatgtgccg
     3421 gtgttgttgg ttggattttt cccatggagt gcctttctcc cttccgtgtt gtatcacacg
     3481 ttcaagcgct ggaaagcgtt ctggcaggga caaggcccag ccacgcaaga gcaacattta
     3541 gagtgtttcc tgagcctctg ggtggtggga ttgttgctgt tctttacctt ctcggccacg
     3601 cgcctgcctc attacatcta ccctttgttt ccggccgcag ccctgctggt cgcccagtgg
     3661 tggaagcgtt tcttgacaga agaaaggccg gctggactca cggcctccac tcgtgtcctc
     3721 attggggtgg gatacctctt gggaatcgcg atgatgtttg tgccggcggt ctttcagttg
     3781 ttcatgaagc aaattgccaa agaattcccg gctgcgatcc aggtggatct ggcgtggctt
     3841 ccctcgctgg taggcctcgt cctggtacta ggaaccatgc tcatgcgtca gtgcatgcga
     3901 tctgactcca agcgccattt ggccttttgg gtaggatgcg gaataatgct gatctttacc
     3961 gtattggtcg cgcgaggagg actctcgatt taccaacagt atttcatcgg accaccgcag
     4021 gaattagccg tcatcgccgg ttataactta ggcccggatg accgattgat tcaatttggc
     4081 cggaagcgcc catccctcag tttttatgcc aaacgcaaag tgtactttct tggggtgaat
     4141 gacgaagagc ttccccaaca cctctctgct cccggccgaa aaatggccat tatccaaact
     4201 cagcttcgcg gacaacttcc cgaagccatg gcccattgga ccgtcgttct ggaccatggc
     4261 ggtttttctc tcctcaccag cgaacccttg ctaaaagata actcgtaagc ttttcatttc
     4321 tcagttagaa ttttttctga taacaaatag ctattccaaa taatttccaa acgattgttg
     4381 tctttgagtc gtgccagaag aagctgcctg caccatggaa ttccctgagt cccctatcct
     4441 tcccattgtc cgacgcctgt tgcccgtcct tatggtcgaa cgctcttatc cctggcgggc
     4501 cttgtgtaag cccttccaca aaacttaggg tagactccgc caattggtcc gccttcccgt
     4561 gctttgcctc cgtccaatct gatgaggccg ggtggggcgt caatggcttg gggtcctttt
     4621 gctctacaca aagggtgcgc tcctcgagag gcatcaaatg atagttgcgc ccttctggac
     4681 gcgccaagca aaacctcggc tgcggggccg aaatcccgca tttctctttt attcacatca
     4741 ttcccagtgc gttaaatgta gttgtaatca catttttttc gctccgaaca aagggaaagt
     4801 ttattggaat gactataaag tgtctggctg agggatgatt tcatcacgca cgtgacatac
     4861 tttatttgtg gaaagaaatg cagaggcaat ggggttcagg aaaaatttta taacccggtt
     4921 gaaaaagttt gccgggattg ccaatagcct ggttggcgat gcccgtgagc tacagcaacc
     4981 agaatgattt tctctccacg cacgaagtaa gggatgtagt aggggaaaat ggggcagttt
     5041 actcgacgat aacccccttc tcgctcttgc cataaaaaag gggtttcagc gaaccgatca
     5101 accacgtagc cgacttcgtt tcgaaaccgt ctgccgagta cgggttcttt ggattcgtgc
     5161 caaagtgcgg cttcaatgag ttcctggcg
//
LOCUS       DATIAL010000282         5514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_697071, whole genome shotgun sequence.
ACCESSION   DATIAL010000282 DATIAL010000000
VERSION     DATIAL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697071"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VLA60_08695"
     CDS             complement(<1..1460)
                     /locus_tag="VLA60_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09479.1"
                     /translation="MNTHLRFMVSMVLGIVAWYGLALADPVLLQEGLGRHHFPISSNG
                     AMAQRYFDQGLILSFGFNHAEAARSFKEAQRRDPNCAMCYWGEALVLGPNINAPMDPS
                     VVPQAFAALEKAVALKDQATEKEAALIQALAMRYSKKVMTDRSPLDVAYAEAMRTVAQ
                     QFPDDPTIGALLAEALMDLHPWDFWSRGGEPKPWTPEIVSRLETVLDQSASHPLANHL
                     YIHIMEASPHPEKALPSAERLPALVPGSGHLVHMPAHIYIRVGRYRDAVLANQHAVKV
                     DEHYLNHAHEESLYTAAYVPHNSHFLWAAAIKLGQQQLAMQAALDTAATVKPEMMRAP
                     GIGGTMQHFWTIPLYTKIAFGQWSNILGEPAPPVDLRYPTAIWHYARGIALVRQGKLE
                     AAPQESMKLAEIARDPAVAKLTVFDLNNIVDILAIAEAVLSGEIAAGHGNYEEAVRQL
                     RRAVELEDGLNYTEPKDWYLPSRQVLGAVLLEAGNVV"
     gene            1686..2486
                     /locus_tag="VLA60_08700"
     CDS             1686..2486
                     /locus_tag="VLA60_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF09480.1"
                     /translation="MFGTDISMDLVIQYGLQAASALLLLAIGAFLARSAGQYAHRSLA
                     KIEMDVPIRVLLVRIIKGIIFLFTLLVVLQQFGIQIFPLIAGLGVAGVGIGLALQGVF
                     LNLFAGLSIIFTKPFRVGEFIDILGEHGEVQAIDIFTTKLLHLDRSIVVIPNRKIIGE
                     ILHNYGKIRQLDLTVGVAYATDLTQAQASVRKIVAANPRILQDPTPVIGIALLGDSSI
                     NISVKPWVTIPDYVPAQAELYQSIVEEFRTIGIQIPFPQREIRMLKAE"
     gene            2589..2918
                     /locus_tag="VLA60_08705"
     CDS             2589..2918
                     /locus_tag="VLA60_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HSF09481.1"
                     /translation="MTYLYLFLAIVAEVIATSSLKAAEGFTKLGPSLLVVIGYFAAFF
                     LLSLVLRSMTVGIAYAIWSGVGIILLALVGAVAFREIPDTPAVIGMGLIIAGVIVIHV
                     FSETVRV"
     gene            complement(2937..4004)
                     /gene="corA"
                     /locus_tag="VLA60_08710"
     CDS             complement(2937..4004)
                     /gene="corA"
                     /locus_tag="VLA60_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HSF09482.1"
                     /translation="MMKGHRKKRSSKSGLPPGTPVHIGEQKLEKMSLTALTYSEHAFH
                     EIHTHRVEEVVPLAGIAEHVWITVQGIHHIEGLQQLGTAFGLHPLVMEDIVNTHQRPK
                     IEDYGEYLFLVMKAVSRKDPAQSLMVEQVSLIVGKNFVLCFLEGNDDPFVNLRERLRN
                     EKGRLRKMGCDYLTYAHLDMIVDQYFPLLEQLGEDIEQLEEQLITQADREALRMLHSF
                     KHEMILLRRAIWPLREVLSHLERGDSTLIHSSTHIYLRDVYDHTIQLIETTETYRDML
                     AGMMDIYLTSLSNKLNETMKVLTVIATIFIPLTFIVGIYGMNFEVMPELEWPWGYPLV
                     MSLMLVLAIGMLWAFRKKRWI"
     gene            4342..5358
                     /locus_tag="VLA60_08715"
     CDS             4342..5358
                     /locus_tag="VLA60_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF09483.1"
                     /translation="MRNFDQTLMSRRRLLQLSGTLAMAPLFLATQSARAVMWSQLFGS
                     TKRTTSPITSNDEFYITSYRTPPFVPADRWALTIRGTVISPFTLTYRDLLAQPAITEI
                     VTLECVGNGVAGEAIGTAEWQGVRLKALLDKGRVTSDAQDVVFHAADGYSDSLTIERA
                     MMDDIMVAYRMNGVHLPLGHGFPARMIVPGHYGMKHVQWLTGIELVSSDYKGYYQRKD
                     WSDEAIVKTKSWITDPQTGDTLPAKNLFTMQGFAFAGSRGIRRVDISTNGGESWAAAT
                     LAPSLSPYSWVMWSYPWEPQRPGDFHILARATDGTGEQQSAQEHPPFPDGASGLQEVI
                     VSII"
BASE COUNT         1307 a         1553 c         1323 g         1331 t
ORIGIN      
        1 acaacatttc cggcctccag gagcaccgca cccaacacct ggcgcgatgg aagataccaa
       61 tccttcggtt ccgtataatt caatccgtct tcaagttcga ctgcgcgccg caactgacgc
      121 acggcctcct cgtaatttcc atggcctgct gctatttctc ctgacaaaac cgcttccgca
      181 atggccagga tatcgacgat attattcaga tcaaaaacag tcagtttagc caccgcaggg
      241 tcacgggcaa tctccgcgag tttcatggat tcctgtggag cagcctccag tttcccttga
      301 cgtaccaatg cgattccccg cgcataatgc cagatagccg tgggataacg taaatccact
      361 ggaggcgcgg gctcaccgag aatgttcgac cactgcccga atgcaatttt ggtatagagg
      421 ggaatagtcc aaaaatgttg catggtaccc ccgatgcctg gagcgcgcat catctcaggc
      481 ttgacggtcg cggccgtatc aagagccgcc tgcatcgcca actgctgttg cccgagctta
      541 atcgccgccg cccagaggaa atgggagttg tggggaacat aggccgccgt atacagactt
      601 tcctcgtgag catggttcaa atagtgctca tcgactttga ccgcatgttg attcgccaac
      661 acggcatccc gataacgccc gacacgaata tagatatgcg cgggcatgtg cacgagatgt
      721 cctgagccag ggacaagggc gggtaatcgt tcggcactgg gaagagcctt ctcgggatgc
      781 ggcgaggctt ccatgatatg aatgtagaga tgattggcca acggatgact ggccgattga
      841 tctaacacgg tctccagcct cgacacaatt tccggtgtcc agggtttagg ctctcctccc
      901 ctactccaaa aatcccaggg atgcaggtcc atcagcgctt cagctaataa cgcgccaatg
      961 gtcgggtcgt cgggaaactg ctgtgcgaca gtccgcatcg cctcggcata ggcgacgtcg
     1021 agtggggaac gatcggtcat caccttttta gagtagcgca tcgccagcgc ctgaatgagt
     1081 gcggcctctt tttcggtggc ctggtctttc agcgcgacag ccttctccag ggccgcgaag
     1141 gcctggggaa cgaccgaagg atccatcggt gcattgatat tcggaccgag caccaacgcc
     1201 tcaccccaat aacacatggc gcagttcgga tcgcgtcttt gagcttcttt gaaagacctg
     1261 gctgcttcgg catgattaaa gccaaaggac aggatgagtc cttgatcaaa atatcgctgg
     1321 gccatcgctc cattggagga gatcggaaaa tgatgtctcc ctaagccctc ttgaagcaga
     1381 acagggtctg ccaaggcaag accataccag gccacaatcc ccagaaccat tgacaccatg
     1441 aatcgaagat gcgtgttcat tgcattcctc ctcttttcac catcctagta ccacatctct
     1501 caccatcgcc tttcttagcg caatctccct atttggcgca acccattttt ctggacattc
     1561 ttgggccatt catatgtctc tcaaaaatac catcctgatg gctcctcatg tagtcatccc
     1621 gtcacttttc cggtaggcta tcccgcgtag agaccgggcc atcaccctca cctggaggac
     1681 cagacatgtt tggaacggat atcagcatgg atttagtcat tcagtacgga ttacaagccg
     1741 cgagcgcatt actgcttttg gcaatcgggg ccttcctggc cagatcggct gggcagtacg
     1801 cccatcgctc cttagcaaaa attgagatgg atgtccctat cagagtgctc cttgtacgaa
     1861 taatcaaggg tatcattttt ctgttcaccc tgctggtcgt gcttcaacaa ttcgggattc
     1921 agattttccc cctgatcgcc ggcctcggcg tcgccggcgt cggtatcggg ttggcgttac
     1981 aaggcgtgtt cctcaacctc tttgcgggac tgagtatcat attcaccaaa ccgtttcgtg
     2041 ttggggaatt tatcgatatc cttggagagc atggcgaagt tcaagcgatt gacattttta
     2101 ccaccaagct acttcatctg gatcgttcca tcgtcgtgat tcccaaccgg aagatcattg
     2161 gcgaaatctt gcataactat gggaagatcc gccaacttga cctgacggtt ggcgtggcct
     2221 atgcgaccga tctaacccaa gcccaggcct ctgtgcgaaa gatcgtcgcg gctaacccac
     2281 gcatattaca agatccgaca ccagttattg gcattgccct tttgggagat tcctcaatta
     2341 acatatccgt caaaccgtgg gtaaccatcc cagattatgt gccagcccaa gctgaactgt
     2401 atcagagcat tgtggaagag ttccgaacta taggcattca gatccccttc ccacagcggg
     2461 agatccggat gctcaaggcg gaatgagcct ccgggcggcc aattcgttgg accatggcac
     2521 ccttaaaaca ggtgattgcg taaaagatgg cctcctcgaa agagggaaag gcagaaagga
     2581 ttgtctcgat gacgtacttg tatttgttcc tggctattgt ggcggaagtg attgcaacca
     2641 gttcactcaa agcagccgag ggtttcacca aattaggccc aagcctgtta gtcgtcattg
     2701 gatattttgc ggcatttttt ctgttaagcc tcgtgttgag gagcatgacg gtcggcatcg
     2761 cttacgcgat ctggtcgggc gtcggcatca ttctcctggc gctcgtgggt gcagtcgcct
     2821 ttcgggaaat tcccgatacg ccggcagtca ttggaatggg attgatcatc gccggcgtga
     2881 ttgtcattca tgtcttttcg gaaaccgttc gggtataggg ctcccgcccc cacttcttaa
     2941 atccaacgct tctttcggaa agcccacagc atgccgatcg cgagaacgag cataaggctc
     3001 atcacgagcg gataacccca cggccattcc aattccggca tcacttcaaa gttcatccca
     3061 tagatcccca cgataaatgt caacgggatg aaaatcgtcg caatcacggt caaaaccttc
     3121 atggtttcat tcagcttgtt acttaagctg gtgagataaa tatccatcat gcccgccagc
     3181 atatcgcgat aggtttccgt ggtttcgatc aactgaatgg tgtgatcgta cacatcccga
     3241 agataaatat gcgtggacga atgaatcagc gttgagtcgc ctcgttccaa atgactgagc
     3301 acttcccgaa gcggccaaat cgctcgacga aggaggatca tctcatgttt aaaggaatgt
     3361 aacattctca gcgcctcccg atcagcctgc gtgatgagtt gttcttcaag ctgttcaata
     3421 tcttctccta attgttccaa cagaggaaag tattgatcca cgatcatgtc aagatgagca
     3481 taggtgagat aatcacaccc catttttcgt aaacggcctt tctcattgcg caaacgctcc
     3541 cggagattaa caaaagggtc atcgtttcct tcaaggaaac acaacacaaa attctttccc
     3601 acaatcaggc tgacctgttc caccatcagt gactgagcgg gatctttcct tgacacggcc
     3661 ttcatcacca aaaacagata ctcgccatag tcttcaattt ttggtcgctg atgtgtattg
     3721 acgatatctt ccatcaccaa aggatgcagt ccgaatgccg tacccaattg ctgaagccct
     3781 tcgatatgat ggatgccctg aacggtgatc cagacgtgtt ccgcgatacc tgccaacggc
     3841 acgacctctt ctacccgatg agtgtgaatt tcgtggaatg cgtgctcgga ataggtcaac
     3901 gccgtcaggg acatcttctc gagcttctgt tcaccaatat gcacgggtgt ccctggcggc
     3961 agccccgatt tactggatcg tttttttcga tgccctttca tcatctgctc cttcgataat
     4021 ctccatcaaa aaacgtcagg cgtaaccggc tctcattgtc tcacagacca tgatcggttt
     4081 gcataaaaag agcgatcagg gaaaactccc gaatcggcct tctttgctcc gtcttctcac
     4141 aatatttccc cgggcagcac ctctcgctct caaatagatt cccggtacca tgaagctccc
     4201 cttcaagaaa tcagccccat taatccctga acacaccacc cgatcctgct cactgttcag
     4261 accagattga tcaacctcga acatctttcc ggcacgactt cactgtaacg tcttcattta
     4321 aaaagagacc atatctctaa catgcgaaac ttcgaccaga cattgatgtc ccgccgccgg
     4381 ctcctgcaat taagcgggac cctggccatg gctcccttat tcttggcaac ccaatcggcc
     4441 agggccgtca tgtggagcca actgttcggt tccaccaaac gaaccacgag tccaatcacc
     4501 tcaaacgatg aattttatat cacgtcttac cgtacccctc cctttgtacc tgctgatcga
     4561 tgggcgctga caattcgagg aacggtcata tccccgttta cccttaccta ccgggacctg
     4621 ctcgcccaac cggcgatcac tgaaatcgtc acattggaat gtgtcggcaa tggggtcgcg
     4681 ggagaagcga tcggcacggc tgaatggcag ggagtccggc tcaaagcgct cttagacaag
     4741 ggcagggtca catcagacgc ccaagacgtg gtctttcatg ccgccgatgg atactccgac
     4801 agcctcacca tcgaacgggc catgatggat gacatcatgg ttgcctatcg catgaacggg
     4861 gtgcacctgc ctttggggca tggatttcca gccagaatga tcgtacccgg gcattacggc
     4921 atgaagcatg tccagtggct cacgggaatc gagctggtgt cctcggatta caaaggctat
     4981 taccaacgta aagactggtc ggatgaggcc atcgtcaaaa ccaagtcctg gatcacggat
     5041 ccgcaaaccg gagatacctt gcccgccaaa aacctattca ccatgcaggg ctttgccttt
     5101 gcgggatccc gggggattcg tcgggtggac atcagcacca acggagggga atcctgggcg
     5161 gccgcaaccc tggctccgtc actttctccc tactcatggg tcatgtggag ttatccatgg
     5221 gaaccgcaaa gacctgggga cttccacatt ttggctcgag cgaccgatgg aacaggggaa
     5281 caacaatcgg cgcaagaaca tccgccgttt cccgacggag cctcgggctt gcaagaagta
     5341 atcgtttcaa ttatctaaga accgggccct gccaacatta tggaatggat cacgcgattt
     5401 tcatgcccgg ggagagcgcc cttcattttt atctcctcct tcgtcctttc catgggctgc
     5461 tcgactattc ccaatacgtt tactcctact aatcctattt ctccggaaac tttt
//
LOCUS       DATIAL010000283         9999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1127062, whole genome shotgun sequence.
ACCESSION   DATIAL010000283 DATIAL010000000
VERSION     DATIAL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127062"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /gene="lpxK"
                     /locus_tag="VLA60_08720"
     CDS             <1..1003
                     /gene="lpxK"
                     /locus_tag="VLA60_08720"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HSF09484.1"
                     /translation="YGAVGHLRRTAYQHGWFPQHRLPKPVISVGNMTVGGTGKTPLVM
                     WLAAGLHARGKRVAILSRGYGRQRSVVNRLVSDGVSLKGDWRSVGDEAMLMAEKCPWA
                     IVAVGTDRYRLGQWVLEQASCDCFLLDDGFQHLSLYRDLDVLVFDATDVEGLSGVVPA
                     GRMREPLSAAKWATTIVFSRTERVVSVASLQSRIEQSVGTSIVPLRLETEPGMFRHIA
                     TGKSQQAGAFKGASCLLFSGIGNPASFRTSVVSCGLTVVEEVRYPDHFAYSEKDVEAI
                     RLKMRRLGLALALTTEKDALKIREHLRPDDPLWALEVQVRMTQGEDQLREQLHTICP"
     gene            1072..2157
                     /gene="waaF"
                     /locus_tag="VLA60_08725"
     CDS             1072..2157
                     /gene="waaF"
                     /locus_tag="VLA60_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HSF09485.1"
                     /translation="MNILVRVPNWIGDAVMCLPALMDLRNHHPHAKVTVLARPIIGEL
                     LEGHPGVDEVMMYVHQGEHQGLLGLWHLIQLVKRKKFDRAVLFQNAFEAALIAWVAGI
                     PSRIGYATDGRRWLLSEHVPRPDRATLHHTAYYRELVKAITRSPGKDGTPQLFLSPEV
                     KHACARQFPEIFLPSDGLVIGINPGSVYGSAKRWIPERFAEVGDRLVERLTEEFPGAP
                     FVRCVLIGGKGEEALAQDIARRMRYEPIALSGRTTIRELMGVLTRCSVLVTNDTGPMH
                     VAQALGVPVAAIFGSTDPSTTSPHGQFSGVVTASVRCAPCLLRACPIDHRCMTQVSVE
                     QVVDVALSQIRESSGYPNEGRIRGHVG"
     gene            2154..2756
                     /locus_tag="VLA60_08730"
     CDS             2154..2756
                     /locus_tag="VLA60_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF09486.1"
                     /translation="MRNESAARGTAVFLDRDGTLNHDTGYVTSPEQLVLFPGVPEAIA
                     RLNHLGVMVLLVTNQSAIGRGMMTLEGLESIHERLAEVIRPYGARIDGIFFCPHHPQD
                     GCGCRKPKAGLIHQAVARFALDVSLCYLVGDKRSDLEAARTASVPGVLVMTSSYSADV
                     VRARDAGQVPIDYVADSFVHAVDWIEQQIMNRDNRITQNR"
     gene            2832..3932
                     /locus_tag="VLA60_08735"
     CDS             2832..3932
                     /locus_tag="VLA60_08735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSF09487.1"
                     /translation="MDITPQRILVIKLSSLGDIVHALPAVAALRQRFPHARLTWMVKE
                     IWAPILEGNPDIDDILSVNVSWQNWPHIFRTLRRGQFDLVVDFQGLFRSGIFGVITGA
                     RTRVGFARAREGATWLYTHRVPLPETKPSPWRLLEVHAVDRNVAITTFLGGRSSTPVF
                     HLPQSSTDRLIIETMLQDAHVLDHEHLIALAPWTRSAMKSWPFKRFVDLAIKLVQWPD
                     VRVVLLGGPSDISAAGEFDCLVPQGLINLVGRLSLPQLPSLLRRMHLLIGNDSALIHL
                     AAGVGIPVLAIFGPTHPKATGPYPLGKHVIVRRELPCSPCGARTCRNPNYLECMKSIS
                     LEDLFREVKGIVGGFSHRRDDRMPATQSSAKP"
     gene            3963..4730
                     /locus_tag="VLA60_08740"
     CDS             3963..4730
                     /locus_tag="VLA60_08740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF09488.1"
                     /translation="MTEQRTREKVSAVVIAYNDAPNMRKCLESLHWVDELVVIDSHST
                     DGTTDICREFTSKIFHYPFQGFGVLRNHALTHAAHDWVLSLDTDERVTPTVQEEIERL
                     LREGPSAQAYKIPRRNFFLGRWIKHCGWYPDYRQPQFFHKGHMRYREELVHEGYELKG
                     KLGYLHASLEQIPFRDIDHYFRKMDRYSSLRAEDMHREGRTFRPHQLVLRPLFTFLKM
                     YVFRGGMLDRKPGLILSMLYAYYTFVKYAKLWEMQKS"
     gene            4816..5940
                     /locus_tag="VLA60_08745"
     CDS             4816..5940
                     /locus_tag="VLA60_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSF09489.1"
                     /translation="MRILHTESSSGMGGQEYRVLEEAQGMEKRGHTVVVAAPHGSQLA
                     ALAEQRGLQVKRTTSGKRGWITLVPSYLRILKHYEIDVVNTHGSLDSWTAAIAGRISS
                     RRPIIVRTRHKSTPVSRTWRHRLLYGKLPHVVTTTGEAVRQGLITGNRLSPSRVISIP
                     TGVDLERFHPQPPDASLRKSLGLGSQGPLIGAVTFLRPEKGMAVLIEAVRWLMQRFST
                     VECIIIGDGGEQPALLGRIRELDLEHCVHLIGFRQDVPALLAILDVVVIPSFEEGIPQ
                     SLTQALAMERPVVASAVGGVPEVVQDGLSGLLVPPRDPKILAEKIACLLSDPIAATRM
                     GKIGRQVIHKRFSMEHMLTQTELVYRQLMRVHSPVVAKRS"
     gene            5998..6768
                     /locus_tag="VLA60_08750"
     CDS             5998..6768
                     /locus_tag="VLA60_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HSF09490.1"
                     /translation="MVCLTGDVHQRSYRGTDTPFSSQTEVALAAKYCDIAAKYGVKVT
                     LFFTGKACQEEPDTVKILSGLPHCEIGGHTFAAFRDPLSRIFKKVYGTPWGTTAHQLR
                     DIQRTIASIQQVTGQRIQTWRNHSYIRTADTDRLLASCGIHVVSDEVSDLKMSAEWMH
                     PGYRSVPMNVLPDHEHVLHGKYQHGKTKPERLTRRMPITEWADQVKASIRSICERGGT
                     ATVLAHPMCMDVADGMVVFEELCRYIQPYGTVWLSEVQ"
     gene            6933..7796
                     /locus_tag="VLA60_08755"
     CDS             6933..7796
                     /locus_tag="VLA60_08755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF09491.1"
                     /translation="MKNQNSPARKNVTSVKRETVSCAVVAFNEEENLQPCLESATWMD
                     EIVVVDSFSTDRTMDIARTFTDKIFQRPWQGFGDQKNYAMDQATTDWVFILDSDERIP
                     AELQAEIEAVLSAGSKDGPVAYYVPRHNYYFGSLVLNAGCYPDYQLRLFRRGIGHLDD
                     AEPHNKFIFSGESANLSCPLVHLTRPTLHNYFEKFPNFTTLAAQDRARTKRHVRGTDL
                     LFRPIFTFSKYYFARKAYRDGMAGFLVSALSSMYTFVKYAKLWNMRQLESQGCTQEPK
                     SQTVTSLSGRQ"
     gene            7843..8946
                     /locus_tag="VLA60_08760"
     CDS             7843..8946
                     /locus_tag="VLA60_08760"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HSF09492.1"
                     /translation="MRIGFDAGPMRSAYSGIGQYVRCLFPAMFSFSQDIEWEAYASSS
                     GSFQTFPFHFPSHVSVKCSKSSWGLRRMEKDQPPLDIFHGTNFKAPDYGQRHTILTIH
                     DVWLARHPEYSKKLFGQALSSWKLGLRARQVSRVVAVSKFSSREIQEVLHLPSDRIVV
                     IHHGPSPDMFPDRDDQKFQEVRTRLHMPVRPFVLFVGGAEPRKNHSAVFKGFARSPRL
                     ASSCSLVAIGELESRGASLMVTARDLGIMDSVCCPGYVSNEDLRQLYSHAEAFVFPSF
                     YEGFGIPLLDAMACGAPIITGTGSALPEVAGDAALYVDPHDPEQLGMETERLVGDREL
                     QTQLRNKGFERVKQFTWERAAQDTLNLYREVRG"
     gene            8972..9292
                     /locus_tag="VLA60_08765"
     CDS             8972..9292
                     /locus_tag="VLA60_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09493.1"
                     /translation="MAWITSWPAWMVLLSVGVTVGLPRVPYYQEGLPYIYALGGMALV
                     TWYIGVGFLILGLYFLVMGCVSAGWFLSKRLHAALFGGVALILAGGCHWFYLLLLEMS
                     VRVT"
     gene            9403..>9999
                     /locus_tag="VLA60_08770"
     CDS             9403..>9999
                     /locus_tag="VLA60_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09494.1"
                     /translation="MTMTLLAKWSTNLPGWGDVIASENGPVEMMSAGVWFVATVWCLT
                     AGLSHSRHRMEWLGLTALCLLFGLRELDAHVWSTGWNLDKVANYWNPSFPLWERLLLF
                     GGMIIPVMVIGIVLCFRIRTRLAAAWNSRAPWLGQLILGGLLLIFCVVVDKLHGYYLP
                     LLGLEDAQLFFMALEEFGEYVLGIYTVSVLWPYWQATLP"
BASE COUNT         2163 a         2386 c         2891 g         2559 t
ORIGIN      
        1 ctatggtgca gtcggccatt tgcggaggac ggcctatcaa catggctggt ttcctcaaca
       61 tcgattgcca aaacccgtga tcagtgtggg taatatgacc gtggggggaa ccgggaagac
      121 tccattggtg atgtggttgg ccgccggact gcatgcgcgg ggcaagcggg ttgcgatttt
      181 aagccgaggg tatggaagac aacgttcggt ggtaaatagg ctggtctctg atggcgtttc
      241 gctgaaggga gattggcgat cggtagggga tgaggccatg ttaatggccg aaaagtgccc
      301 atgggcgatt gtggctgtgg gaaccgatcg ttatcgtctg ggacagtggg tgttggagca
      361 ggcctcatgc gattgctttc ttcttgatga tggcttccag catctttctc tctatcgcga
      421 tctggacgtg ctcgtatttg atgcgaccga tgttgagggc ttaagcggag tcgtgcccgc
      481 cggccgaatg cgtgaaccgc tgtccgcagc aaaatgggcc acgacgatcg tcttcagtcg
      541 aacggaacga gttgtatcgg tcgcatcact tcagagtcga atcgaacaga gcgtgggaac
      601 gtccattgtc cccttgcgtc tggaaaccga acccggaatg ttcaggcata ttgcgacagg
      661 gaaatcgcaa caggccggag cctttaaggg tgcatcctgt ttgctcttta gcgggatagg
      721 caatcccgcc tcctttcgga cgagtgtggt ttcttgtggg ttgacggtag ttgaggaggt
      781 tcggtacccc gatcattttg cctattcaga aaaagatgtg gaagccattc gtttgaagat
      841 gcgacgttta gggcttgctc tcgcgctgac aacagaaaaa gatgctctga aaattagaga
      901 gcatctgaga cctgatgatc ctctatgggc gctggaagtg caagtccgga tgactcaagg
      961 ggaagaccag ttacgtgaac agcttcatac catttgtcca taggactcag atatggttga
     1021 tcagataggg tcaagcagat cgattcatgt gagagggcat agcctttttc gatgaacatt
     1081 ctcgtccgcg ttcccaactg gatcggcgat gccgtgatgt gcctaccggc tctcatggat
     1141 ttacggaatc atcatccgca tgccaaggtc acggtgttgg ccaggccgat tatcggagaa
     1201 ttgttggaag gacatccggg cgtggatgaa gtcatgatgt atgtccatca aggggaacat
     1261 cagggccttc tggggctctg gcacctcatt caacttgtga aaagaaagaa atttgatcgt
     1321 gcggtgttat tccaaaatgc ctttgaagcg gccctcattg cctgggtggc tggcatccca
     1381 tcccgcatcg gatatgcgac ggacggacgg cgttggcttc tgtccgagca tgtgcccagg
     1441 ccggatcggg ctaccctgca tcatacggcg tattatcggg aactggtaaa ggccatcacc
     1501 cgctctcccg gaaaagacgg gacccctcaa ctttttcttt ctcctgaggt gaagcatgcc
     1561 tgtgccagac agtttcctga gatatttctt ccatcggacg gtctggttat tgggatcaat
     1621 cccggttcgg tgtatggatc ggccaagcga tggataccgg aacgtttcgc ggaggtgggt
     1681 gatcggctgg ttgagcgtct gacagaagaa tttccgggtg ctccgtttgt ccgatgtgtc
     1741 ctgattggag gcaaaggaga agaagcgctt gcgcaggaca ttgccagacg gatgcggtat
     1801 gagccgattg ccctgtcagg aagaaccacc atacgggaat taatgggagt gttgacacgc
     1861 tgctccgtct tggttaccaa cgacacggga cccatgcatg tggcccaggc tctcggggta
     1921 ccggtcgcgg cgatttttgg gtcaacggat ccctccacga caagtcctca tggacaattc
     1981 agtggcgtgg tgacggcatc ggtccgttgt gcaccctgtt tattgcgcgc ctgcccgatt
     2041 gatcatcgtt gtatgaccca agtgtccgtt gagcaggttg tggacgtcgc gctgtctcaa
     2101 atcagggaat catcgggcta tccgaacgag ggtcgaatca gagggcatgt tggatgagaa
     2161 atgagtccgc cgcacggggt acggccgtgt ttttggatcg ggatggcacc ttgaaccacg
     2221 atacggggta tgtcacttct ccagaacagt tggtgttgtt ccccggagtc cctgaagcca
     2281 tcgcccgatt aaaccactta ggggttatgg tgcttctggt caccaatcaa tcggctattg
     2341 gtcgggggat gatgaccctc gaagggttgg agtcgatcca tgagaggctg gcggaagtga
     2401 ttcgtcccta tggagcccgg attgatggta ttttcttctg cccacatcat cctcaagacg
     2461 ggtgtggatg tcgaaagccc aaagcgggat taattcatca agcggtcgct cgatttgctc
     2521 tcgatgtatc gctgtgttat ctcgtcgggg acaaacgcag tgatttagag gcggctcgca
     2581 ccgcctcggt acccggtgtc ctggtcatga cgagttcgta tagtgcggat gtggtaaggg
     2641 cgagagacgc gggacaggtg ccgattgact atgttgcgga cagctttgtt catgccgtgg
     2701 actggataga acagcagatc atgaaccgag acaaccgaat cacgcaaaac cgataaagcc
     2761 gctaattcat tcatgcgcgt tatcgggata ttgtatgtta tcgatgggct aaaggttttt
     2821 ggcgggtacg tatggacatc actcctcagc gcatcctggt catcaaatta agttcgctgg
     2881 gcgatattgt tcatgccctt ccagccgtgg ctgcgttgcg tcagcggttc ccgcacgccc
     2941 gactgacctg gatggttaaa gagatctggg cgccgattct ggaagggaat cctgatattg
     3001 atgacatttt atctgtgaat gtgtcttggc aaaattggcc gcatatcttt cgtacactcc
     3061 gaagaggaca gtttgatctg gttgtggatt ttcaaggact gttccgttca ggaatctttg
     3121 gcgtgataac tggagctcga acccgtgtgg gttttgcgcg ggccagggaa ggggcgacct
     3181 ggttgtatac acaccgggta cctttgccgg agacgaagcc ttccccctgg cgtttgttgg
     3241 aggttcatgc cgttgatcgg aatgtggcga tcaccacatt tctgggcgga cgctcatcta
     3301 ctccggtgtt ccatctacct caatcctcta ccgatcgtct catcattgaa acgatgcttc
     3361 aggatgcaca cgtcctggac catgaacacc tgattgctct ggcaccttgg acacggtctg
     3421 ccatgaagtc ctggccgttt aaacggtttg tggatctggc catcaaattg gtgcagtggc
     3481 ccgatgtgcg tgttgtgctt ttgggagggc cttcagacat ctcggctgcc ggggaattcg
     3541 actgtctcgt gccacagggt ctcatcaacc tggtcggacg actgtctctt ccccaattac
     3601 cctcactgtt gcgaagaatg catctgctca ttggaaacga ttcggccctc attcaccttg
     3661 cagcgggagt gggcattcct gtgctcgcga tctttggccc cacgcatccc aaagccacag
     3721 gtccctatcc tttgggaaag catgttatcg tgcgcaggga attgccctgc agtccctgtg
     3781 gagcacgaac atgcagaaat cccaactatc tggaatgtat gaaatcgatc tcactcgagg
     3841 atctctttcg tgaggtcaaa ggcatcgtcg ggggcttctc ccatcgaagg gatgatcgca
     3901 tgccggcaac ccaatcctcg gcgaaaccgt aaatctgtga ggaggtcgaa ggaaaaaacc
     3961 gtatgacgga acaacgcacg agagaaaaag tttccgctgt ggtgattgcc tataatgatg
     4021 caccgaatat gcggaaatgt ttagagagtc ttcattgggt ggatgaattg gtggtgatcg
     4081 attctcatag cacggatggg acgactgata tttgccgtga atttacctcc aagattttcc
     4141 actatccctt ccaggggttt ggggtgcttc ggaatcacgc cctgacccat gccgcgcatg
     4201 actgggtctt gagcctggat acggatgaac gggtgacgcc aacggtgcag gaagaaatag
     4261 aacggttgct tcgggagggg ccttcggcac aagcgtataa gattcccaga aggaattttt
     4321 tccttgggcg gtggatcaaa cattgtggat ggtaccctga ttaccggcaa ccccagtttt
     4381 ttcataaggg acatatgcgc tatcgggagg agctggtcca tgagggctat gagctgaagg
     4441 ggaaacttgg atatcttcat gccagtctgg agcagatacc ctttcgggat atcgatcact
     4501 actttcgcaa aatggatcgg tattccagct tgcgggcgga ggacatgcat cgtgaaggcc
     4561 ggacattccg cccccatcag ttggtgcttc gtcccttatt tacctttctg aaaatgtatg
     4621 tatttcgggg ggggatgttg gaccgaaagc cggggctgat tttatccatg ttgtatgcct
     4681 attacacctt tgtgaaatac gccaagttgt gggaaatgca aaaaagttga tcctatggac
     4741 ctcctcggcg atcccgttga tcctcccata actatgtcga aggtaagccc aactttttct
     4801 cgtcatcaga gacctatgcg cattcttcac acggaatctt cttcagggat gggagggcag
     4861 gaataccgcg tgttggagga agcgcaaggc atggagaagc gtggtcatac ggtggtagtc
     4921 gctgctccgc atgggagtca gttggccgcc ctggccgaac agcgagggct ccaggtcaaa
     4981 agaaccacgt ccggcaagcg agggtggatc acattggtcc cctcctattt acgaatcttg
     5041 aagcactatg agattgatgt ggtcaatacg cacgggtctc ttgatagttg gacggcagcg
     5101 attgccggac ggatctcgtc ccgacgcccc atcattgtcc ggacccgtca taaaagcacg
     5161 ccagtctcac ggacgtggcg gcatcgactc ctctatggga aattgccgca tgtcgttacc
     5221 accaccggtg aggcggtcag acagggattg ataacaggaa accggctgag cccttcccgg
     5281 gtcatttcga ttcccacagg agttgatttg gagcggttcc atcctcaacc cccggatgcg
     5341 tccttgcgaa agagtttggg gcttggaagt caaggtcctt tgatcggagc cgtcaccttt
     5401 ctgcgcccgg aaaagggcat ggcagtcttg atcgaggcgg tccgatggct catgcaacgt
     5461 ttttccaccg tcgagtgcat cattatcgga gatggcggag aacagccggc attgttgggt
     5521 cgcattcgag agcttgactt ggagcactgt gtgcacctta tcgggtttcg gcaggatgtg
     5581 ccggctctgc tggcgatact tgatgtggta gtcattcctt cttttgaaga aggaattcct
     5641 caatctctca ctcaagcctt agccatggaa cgtcctgtgg tcgcttctgc ggtgggaggg
     5701 gtgcccgaag tggtgcagga tggcctgagc ggactgttgg tccctccacg agacccaaag
     5761 atcttagccg agaaaattgc ctgtcttctg tctgatccca ttgccgcgac ccgaatgggg
     5821 aagatcgggc gacaggtcat tcacaaacga ttttccatgg aacacatgct gactcaaacg
     5881 gagttggtgt atcggcaatt gatgcgagta cactcccccg ttgttgcgaa gaggtcttga
     5941 acgggtgtgg agcgcgtcgg cgacttagcc gggttgagct acaaaggatt cgagaacatg
     6001 gtttgtttga caggagatgt tcaccaacgc tcgtatcggg gaaccgatac gccattttcc
     6061 tctcaaacag aagtcgcgtt agccgcgaag tattgtgata ttgccgcgaa gtacggagtg
     6121 aaagtcacgc tgttttttac cggaaaagcc tgtcaggagg aaccggacac ggtgaaaatt
     6181 ctatcgggtc tgccccattg tgaaattggg ggccatacct ttgcagcctt tcgagatccg
     6241 ttatctcgaa tattcaagaa agtgtatgga acgccatggg gaaccactgc tcaccaactt
     6301 cgtgatattc agaggaccat tgccagcatt caacaggtga cgggtcaacg aattcagacc
     6361 tggcgaaacc atagttatat acgaaccgct gatacggatc gcttattagc atcctgtgga
     6421 atccatgtgg tatccgatga ggtctccgat ttgaaaatgt ccgccgaatg gatgcatccc
     6481 ggctatcgtt cggttccgat gaatgttctt ccggatcacg aacatgtctt acatggaaaa
     6541 tatcaacatg ggaaaaccaa gccggagcgt cttacccgac gaatgcctat cacggaatgg
     6601 gccgatcagg taaaggcgag tatacggagt atatgcgagc gaggaggaac cgcgacggtc
     6661 cttgcccacc cgatgtgcat ggacgttgcc gatggcatgg tggtgtttga ggagctttgt
     6721 cgatatatcc agccgtatgg gacggtttgg ctctcagaag tgcagtaaag gaaacggggt
     6781 gggaaaatga aatgccgatt gaggagaggt agggtgggat acctgatgcg caggaatcga
     6841 ccagtggacg atgatgcaaa acccgggaat ccgtgcaggc atagactgtc cttattaccc
     6901 ttggtggtta tcgataatgg atcatttatc ttatgaagaa tcagaatagt ccggcccgaa
     6961 agaatgtcac ttcggtcaag cgggaaacgg tctcttgtgc cgtggtggca ttcaacgaag
     7021 aggaaaatct tcagccgtgt ttggaaagtg caacgtggat ggatgaaatc gtcgtggtgg
     7081 attcattcag tacggatcgt accatggata ttgcccgaac gtttacggac aagatttttc
     7141 agcgcccctg gcaagggttt ggggatcaga aaaattatgc gatggatcaa gcgaccacgg
     7201 attgggtgtt tattttggat tccgacgagc gaattcctgc cgaacttcag gctgagatcg
     7261 aagcggtgct gtctgccggt tcgaaggatg gacctgtggc ctattatgtc ccccgtcata
     7321 attattattt tggctctctg gtattgaacg ccgggtgtta tcccgattat caattgcgtc
     7381 tgttccgccg gggaatcggc catttggatg acgccgagcc acacaataaa ttcatctttt
     7441 cgggagaatc cgctaatctc tcgtgtccat tggtacacct taccaggccc acccttcaca
     7501 attattttga aaaatttccg aactttacga cgttggccgc gcaagaccga gccagaacca
     7561 aacgccatgt gcgtggcaca gacctcctgt ttcgtccgat ctttacgttt tcgaaatatt
     7621 attttgctcg aaaggcttat cgcgatggca tggcggggtt tttagtcagc gctctgtcca
     7681 gtatgtatac ctttgtgaaa tatgccaagc tctggaacat gcgacaactc gaatcgcaag
     7741 gatgcactca ggaacccaag tcacaaaccg tgacgtccct gtcaggaagg cagtgatggc
     7801 cgtgagagaa ggtccgatga ttcagaacgg ttcggacacc tgatgcggat cggttttgat
     7861 gccgggccca tgcgttccgc ctatagcggg attggacagt atgtccgatg tctcttccct
     7921 gccatgtttt ccttttctca agatattgaa tgggaggcct atgcctcatc gagtggttct
     7981 ttccaaacct ttccgtttca tttcccgtcg catgtctccg tgaaatgctc caagtcctcg
     8041 tggggtttac gccgaatgga gaaggatcag cccccgctcg atatctttca tgggaccaac
     8101 ttcaaggctc ccgattatgg ccaacgtcac accatactca cgatccatga tgtctggttg
     8161 gcacgtcatc cagagtattc gaaaaaactc tttggccaag cgctttcctc ctggaagtta
     8221 ggtctgcgtg ccaggcaggt ctctcgcgtg gtggcggttt caaaattttc ttcccgggaa
     8281 attcaagagg tgcttcatct cccctccgac cgtattgtgg tgatccacca cgggccgtct
     8341 cccgacatgt ttcccgatcg ggatgaccag aagtttcagg aggtccggac tcgattgcac
     8401 atgcctgttc gtccgtttgt gctatttgtg gggggagctg aaccccgaaa aaatcatagc
     8461 gcagttttta agggatttgc ccgatctcca cgattggcct catcatgctc tcttgtggcc
     8521 atcggtgagt tagaatcacg gggcgccagc ctgatggtga cggccaggga tttggggatc
     8581 atggattcgg tctgttgtcc cggatatgtg tccaacgaag atttgcggca gctgtattct
     8641 catgcggaag cctttgtgtt tccctccttc tatgaaggct tcgggattcc ccttcttgat
     8701 gccatggcct gtggtgcccc gattattacc gggacgggga gtgctctgcc ggaagtcgca
     8761 ggggatgctg ctctctatgt cgaccctcac gatccggaac aattgggaat ggaaacagaa
     8821 cggctggtgg gtgatcgcga attacaaacg caactacgca acaaggggtt cgagcgggtc
     8881 aagcaattta catgggagcg ggcggcacag gacacgctga atctctatag ggaggtgcgt
     8941 ggctaaccca aggtgtttta tgaaaatggc aatggcttgg ataacctcat ggccggcctg
     9001 gatggtattg ctatcggtag gtgtaacggt agggcttcct cgcgtgccgt attatcagga
     9061 gggtctcccc tatatctatg ccctcggggg aatggcctta gtgacctggt atattggggt
     9121 cgggtttctc attcttggac tctatttcct cgtcatggga tgtgtgagtg cagggtggtt
     9181 cctgagcaaa agactccatg cggccctctt tggaggcgtg gctttgattc ttgccggcgg
     9241 ctgtcactgg ttctatctgc tgttgttgga gatgtcagtc cgagtcacat gagaccggga
     9301 tttcctcaaa tgcccccgga caaaccttca ctcctcccgg atacacgtca aggacgggcc
     9361 tggctgtgtt ggaggaacct tgtggaggcg ctcttttttt gtttgacgat gacgcttttg
     9421 gcaaaatggt ctaccaattt gccggggtgg ggggatgtca ttgcctcaga aaacgggccg
     9481 gttgagatga tgagtgcggg cgtatggttt gtcgccacgg tgtggtgcct gaccgcgggc
     9541 cttagtcatt ctcgccatcg catggagtgg ttggggctca cggctctctg tcttctcttt
     9601 gggctgcggg agttagatgc gcatgtctgg tcgaccggat ggaatttaga taaggttgcg
     9661 aattattgga acccgagctt tccgctttgg gaacgactgt tgttgtttgg gggtatgatt
     9721 attcccgtca tggttattgg aatagtcctc tgttttcgaa tccggacacg actggcggca
     9781 gcctggaata gtcgtgctcc ctggcttggc caattgatcc tgggaggcct gctgctgatt
     9841 ttctgtgtgg tggtggataa actgcatggc tattatcttc cacttcttgg gttggaagat
     9901 gcacagctat tcttcatggc gttggaagaa tttggagaat atgtattggg gatttatacc
     9961 gtgtcggttt tatggcctta ctggcaagct acccttccg
//
LOCUS       DATIAL010000284         6234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_52704, whole genome shotgun sequence.
ACCESSION   DATIAL010000284 DATIAL010000000
VERSION     DATIAL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52704"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VLA60_08775"
     CDS             complement(<1..1277)
                     /locus_tag="VLA60_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09495.1"
                     /translation="MRVEPRILLLAFGAILFVGCSGPMSHYAKVERSLLAGKSAQAVT
                     IIQSAKADYGDNNRLLYLLDEGMVLHLAEQYIESNKVLEEAYTLVEDLYTRHLRDEAS
                     ALLVNEAQRPFEGAPYEHVMINVVKALNYAALQQWNEALVEGRRIDHRLNVLHDKQEG
                     KEAYHEYPFARYLVGLLYDIAGDLNNAYVGYRKAEQAYEDSRAWSHVVFPDILKADLI
                     RTAERLGLSDEVQQYREKYPEVSIASVSDGLDSQAQLIVISYHGQGPKKEDVIIDVPV
                     SLDALYLVALTKPWFGRSTRDTRVGESLLYGIHGQIARIALPRLTVKKTSLAHDVIEG
                     TYQTASFKTQSERVYDIAAVAEKNLADNYNGLMLRAVARSAMKMAAAEGITIGARAAA
                     GRNNQDWVGLLVGAIARIFALATEEADIRSWRTL"
     gene            complement(1401..2024)
                     /locus_tag="VLA60_08780"
     CDS             complement(1401..2024)
                     /locus_tag="VLA60_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HSF09496.1"
                     /translation="MLPITRLIKSCAVVVPCLYLLLLGGCGKEKKVTRVDPGVVTDFS
                     GRWNDTDSKLVAEAMMKEMVSRPWLENFTNQHNRVPTVIVGTILNKSHEHIDVGTFVT
                     DLEREMTNSQKVLFVASQRDRDEIRQERQEQAVHAREDTQRRPGQELGADFMMKGTIS
                     TIVDESDGVKGIYYQVDLDLINVETNVKSWYGQKKIKKVVERKRLLF"
     gene            complement(2081..2653)
                     /locus_tag="VLA60_08785"
     CDS             complement(2081..2653)
                     /locus_tag="VLA60_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09497.1"
                     /translation="MNEQGHDMRRIVLRIGGCALLAFMLVACGGPPKWVQEGSGVMNA
                     DDSKSIYGVGAVVGVKNEPLAWETAENRARAELAKTFRTYTAYLMQDYAASTSAGNFT
                     KSTEEQNVERAVKTFSAVTLNGVRPIDRYKDDETSTYYVLTKLSFAEMKEALQQAQEL
                     DEEVRDFVRKNAEKAFKKLEQEEAKRPGGL"
     gene            2838..3008
                     /locus_tag="VLA60_08790"
     CDS             2838..3008
                     /locus_tag="VLA60_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09498.1"
                     /translation="MADVTIEDGIIRVKELDIQDGKAAKALAEYPEAKWAEITRRAVK
                     IGLGFLKGGAEP"
     gene            complement(3108..3545)
                     /locus_tag="VLA60_08795"
     CDS             complement(3108..3545)
                     /locus_tag="VLA60_08795"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HSF09499.1"
                     /translation="MIQSLKTFQGLAALGVFLLGISSGCTFKATLDTTSDTTTNFLSS
                     TSGKSWWTEDGLVKHGEHARAFVATNHDILLQEIAQGQGEYVQAFGTILGVPSHHQAH
                     FQELIQNEYPVLVEIPIFLGDDQLNRFIGHAQQAGIYAASSNL"
     gene            3718..5274
                     /locus_tag="VLA60_08800"
     CDS             3718..5274
                     /locus_tag="VLA60_08800"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HSF09500.1"
                     /translation="MLTPEDHLELIRRGISTFQVESQLQRLRHGVPPITIIRPCRLND
                     GIVQLRPEHFSQYQQQFEMARQEDRVSKFIPASGAATRMFNDLLKFLSQEGSPESSSN
                     QAPTLPRAVDQAWTRLQDFPFIPDLEQYLHGQGQPPPTDQHTHDINTILQAVLKTPGL
                     GYAELPKALLSFHRYPEGPRTALEEHIHEAIRYHPNQLNSIKIHLTVSPQFEQAIKTH
                     LHTIQQTLGRQGWKLDCTVSFQKPSTDTVALDSTNQPFRGEDGTLVFRPGGHGALLEN
                     LNDYQGDIIFISNIDNVVPDHLKDPIVTWRKALAGYLVELQQQVFHHIAQLSSLPADA
                     KSVHQAEACILQELLLPLPQSYRELALPDQATLLKQYLDRPIRVCGVVPNTGDPGGGP
                     FWVAHPEDAPSRQIVEQSQVNPDSEAQQKLFASGTHFNPVDLVCGVRDFQGNPFNLLE
                     YVDPGTGFIGMKSYQGRPLKALEWPGLWNGGMAHWITIFVEIPRTNFNPVKTFLDWLH
                     PAHQPQTKQP"
     gene            5446..6135
                     /locus_tag="VLA60_08805"
     CDS             5446..6135
                     /locus_tag="VLA60_08805"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HSF09501.1"
                     /translation="MNEQEFFSLWQNFGFPCKSHPWHGVEIGEEAPFTVTVYVEIVPT
                     DTVKYELDKKSGHLRVDRPQRYSNVCPTLYGLIPQTYCGQSVAALSAECTNRPGLVGD
                     LDPLDICVLTEKSIIHGDVLLQAIPIGGLRMIDGDEADDKIVAVLKGDALHEQWTDIK
                     ACPVHFVERLMHYFLTYKDAPGSQKKNCEITHVYGREEAHEVIRRSQQDYAAKYGHLQ
                     AQLAKPRHALN"
BASE COUNT         1590 a         1675 c         1389 g         1580 t
ORIGIN      
        1 agagttcgcc acgaccgaat atcggcttct tctgtggcca gggcaaaaat tctggcaatc
       61 gcacccacta acaagccgac ccaatcctgg ttgttccggc cagcggccgc tctggcccca
      121 atggtgatgc cttcagccgc cgccattttc atggcggatc gagctactgc ccggagcatg
      181 agtccattat aattatcggc caggtttttt tcggctacgg cggcaatatc atacacccgc
      241 tccgattggg ttttaaaact ggcagtctga tatgtgccct caatcacatc atgagctaac
      301 gaggtttttt tcaccgtaag ccggggtaag gcaattcgtg cgatttgtcc gtgaatgcca
      361 tacagcagag actccccgac tctcgtgtca cgagtactcc ggccaaacca gggcttggta
      421 agggccacca ggtacaaggc gtctaaactc acgggaacat caataattac atcttctttc
      481 ttaggtcctt ggccatggta actgatcaca atgagttggg cctgggagtc taggccatcg
      541 gagactgaag caatactgac ttccggatat ttctcccgat actgttggac ttcatcagac
      601 aaccccaatc gttcggccgt tcgaatcaga tcagctttca ggatatccgg aaaaacaaca
      661 tgggaccacg cccgactgtc ctcataggct tgttcagcct ttcgatatcc gacataggca
      721 ttattgagat caccggcaat gtcatagagc agccccacca aataccgagc aaacggatat
      781 tcatggtagg cctcttttcc ctcttgtttg tcatgaagca cattgagacg gtgatctatc
      841 ctccgtccct ccaccaacgc ctcattccac tgttggaggg ccgcatagtt cagcgccttc
      901 accacgttaa tcatgacatg ttcataagga gccccttcaa atggccgttg agcctcattt
      961 acgagcaggg ctgaggcctc gtctcgaaga tgcctcgtat acaggtcttc aacaagagta
     1021 tacgcctctt ccagaacctt attgctttca atgtattgtt cggccaaatg aagcaccatg
     1081 ccctcatcca gtaaatagag caagcgattg ttgtctccat agtctgcctt tgccgattga
     1141 ataattgtaa cagcctgggc tgatttccct gccaacagac tacgctctac cttggcatag
     1201 tgggacatag ggccactaca cccgacaaac aagatggctc cgaaggcaag caggagaatt
     1261 ctcggttcaa ctctcatgga gaagagggca cgttggccaa ggtgggctgg tgaaaaacaa
     1321 ggccctgacg attaaagcca aggaaagaaa atgaccgaac aaccgaccgc acgtgacgga
     1381 agggattgga taaccttgcg ttaaaagagg agacgtttac gctcgacgac ttttttgatt
     1441 tttttctgtc cgtaccagga tttcacgttc gtctcaacat tgatgagatc taaatcaacc
     1501 tgataataaa tccctttgac cccatcggat tcatccacga tagtactaat cgtgcccttc
     1561 atcataaaat ccgcccccag ttcctgccca gggcgtctct gggtatcttc cctggcatga
     1621 actgcttgct cttgtcgttc ctgacgaatt tcatcgcgat cccgttgact tgccacaaac
     1681 agcacttttt gagaattggt catttcccgt tccaagtccg tcacaaaggt gcccacatca
     1741 atatgttcat ggcttttatt gaggatcgtt ccgacaatca ccgtcgggac tcgattgtgt
     1801 tgattcgtaa agttttccaa ccaaggcctg ctcaccattt ccttcatcat ggcttcggcc
     1861 actaactttg agtccgtgtc attccaccga ccgctaaaat cggtcacgac cccggggtct
     1921 actctggtca cctttttttc ttttccgcat ccgcccaata agagaaggta gaggcagggg
     1981 acaacaaccg cgcaggattt gattaatcga gtaatgggga gcaaggtgat tcctttgtga
     2041 tgatgaacca agaaggcaaa aggccgccaa ttaacctggg tcaaagccct cccgggcgtt
     2101 tggcttcttc ctgctccagt tttttaaagg ctttttcagc gttcttgcgg acaaaatccc
     2161 gaacctcttc gtccagttcc tgcgcctgtt gcagcgcttc cttcatttcc gcaaaagaca
     2221 gcttggtcaa cacataatag gtggaggtct cgtcgtcctt gtaacggtca atgggacgta
     2281 ctccatttaa agtcacagcc gaaaaggtct tgaccgcccg ttctacgttt tgctcttccg
     2341 ttgatttggt gaaattcccc gcagaagttg aagcggcata gtcttgcatc agataggccg
     2401 tataggtgcg aaaagttttt gccaactcgg cgcgtgcgcg gttttcagca gtttcccagg
     2461 cgagtggttc attttttacc ccgacgacag ccccgacacc ataaatagac ttggaatcat
     2521 cggcattcat cacacccgat ccttcctgaa cccatttggg aggacctcca catgccacaa
     2581 gcatgaaagc gagaagagca catcctccga tgcggagaac aattcgacgc atatcatggc
     2641 cttgttcatt cattagggta ccctaacctt ttttaagaaa atttttatca gaggttcctg
     2701 tcagacgggc ttagaaaatg ctagcattgg acctacaacc tgtcaagaaa attgaacttt
     2761 ttaccatctc accctgcctc acatcgaatt gcattcatca tcattcgaga catgcaggaa
     2821 accaaggagg atgtattgtg gctgatgtaa caattgagga tggcattatc cgggtgaaag
     2881 aactcgatat tcaggatgga aaagccgcca aggccctggc tgaatatccg gaagccaaat
     2941 gggcggaaat caccagacgt gcggtaaaga ttggcctggg ctttttaaaa ggaggggcag
     3001 agccttgacc atatcctcca tccttttaat agcctaaatg ctttttagat aatgatgaat
     3061 gtctcaaatg ggaaacaccc catgcccggg ttcttaaccc cacccggtta tagatttgag
     3121 gacgcagcat aaattccggc ttgctgggca tgaccgataa acctgtttaa ctgatcatct
     3181 ccaagaaaaa tgggtatctc aaccaggacg gggtactcgt tctgaatcaa ttcctgaaag
     3241 tgagcctggt gatgagatgg gacacctaaa atcgttccga aggcctggac atattccccc
     3301 tgcccttggg cgatttcttg aaggaggatg tcatggttgg tcgccacaaa ggctctggca
     3361 tgttcgccat gtttgacaag cccatcttcc gtccaccagg atttcccgga agtagaggac
     3421 aaaaaattgg tggtggtatc cgaagtcgta tcgagtgtag ccttgaaggt acaaccggaa
     3481 gaaataccca ataggaatac ccctaatgcc gcgagacctt gaaacgtttt caagctttgt
     3541 atcatgatag ttctccttgc ctgagtgcat gaacatgaca gagatgtcat gagactttac
     3601 aattggattt ttgtcgaatg cagcacatta aaactttgac atttttcatc atgaaataca
     3661 acttctatct gatgtgatct ctgatcaatc tcagttaaac tggattaaat aaacgttatg
     3721 cttacgcctg aagatcatct tgagttaata cgccgcggga tttccacgtt ccaggtggaa
     3781 tcacaacttc agcgcttgcg tcatggagtc cctccgatca ccatcattcg tccatgccgg
     3841 ctgaacgatg gtattgtcca actccgtcca gaacacttct cccaatacca acaacaattt
     3901 gaaatggccc gtcaggagga tcgagtcagt aaatttattc ctgcatccgg agccgccacc
     3961 cgtatgttca atgacctcct caaatttttg tcccaggagg gctcacccga atcctcttca
     4021 aatcaggcac caactcttcc gcgcgctgtc gatcaggcct ggacgcgctt acaggatttc
     4081 ccgtttattc ctgatttaga acagtatctc catgggcagg gacaaccccc tccaacggat
     4141 cagcatacac atgatatcaa tacgattctt caagcggtcc tgaagacgcc cggccttggc
     4201 tatgccgaat tacccaaagc cctcctctcc ttccatcgct atccggaagg gccccgaact
     4261 gcacttgaag aacatattca cgaagccatc agatatcatc ccaatcaact aaattcaata
     4321 aagattcatt tgaccgtttc tcctcaattt gaacaggcca ttaaaaccca tttacatacc
     4381 atccaacaaa cgttgggaag acaggggtgg aaacttgatt gcacggtctc cttccaaaaa
     4441 ccgtcgaccg acaccgtcgc cctggactct accaaccagc catttcgtgg cgaagatggc
     4501 actctggttt ttcgaccggg cgggcatggg gcgctattgg aaaatttaaa tgactaccag
     4561 ggagacatca tttttatttc caatatcgac aacgtcgtcc ccgatcattt gaaagatccg
     4621 attgtcacat ggagaaaggc attggcaggt tacctggttg agttacaaca acaggtgttt
     4681 caccatatag cacaactcag ctcgctcccc gcagatgcga aaagtgtcca tcaggcggaa
     4741 gcctgtatcc tgcaagaact ccttcttcca cttccccaat cgtatcgaga attggcgctt
     4801 cccgatcaag ccacattatt aaagcagtat ctagatcggc ccatcagggt ctgtggtgtc
     4861 gtccccaata ccggcgaccc gggcggaggg ccattttggg ttgcgcatcc cgaggacgcc
     4921 ccgtccaggc aaatcgtcga gcaatcacaa gtcaatcccg attctgaagc ccaacaaaaa
     4981 ctctttgcct ccggaactca tttcaatccg gttgatctgg tctgcggagt ccgtgatttt
     5041 caaggtaatc cctttaacct cctggagtac gtcgatcccg gaaccggctt catcggcatg
     5101 aaatcctacc agggtcgtcc cctcaaagcc ctggaatggc caggcttgtg gaatggagga
     5161 atggctcatt ggattaccat tttcgtggaa attccccgca ccaactttaa tcccgtcaaa
     5221 accttccttg attggcttca ccccgcccat caacctcaga caaaacagcc gtaactctgc
     5281 tcccggctgg gaaacatctg tttcgttttt cttcgttcct gttcaatact taccagcatc
     5341 cccggcacgc gtggccggtt aagatgaaga agattccgac cgaaacctag tagaggtcat
     5401 tgcgccttaa ctgggctcac ttatcccaca cctggaaaca ttttcatgaa cgaacaagaa
     5461 tttttctccc tttggcaaaa ttttgggttc ccctgtaaat cccatccgtg gcacggtgtc
     5521 gaaataggag aagaggcacc attcaccgtc acggtctatg tcgaaattgt gcccacggac
     5581 acggttaaat atgagttgga taaaaagtcc ggccatttac gagtcgaccg gccccagcgc
     5641 tattccaatg tgtgccccac gctgtatgga ttaattcctc aaacttactg cgggcaatct
     5701 gtcgcagcac tcagtgccga atgtaccaat cggcctggcc tcgtcggtga cctggatccc
     5761 ttagacattt gcgttttgac tgagaaaagc attatccatg gagatgtttt gctgcaagcc
     5821 attccgattg gcggcttgcg tatgatcgat ggggatgaag ccgatgataa aattgtggct
     5881 gtccttaagg gtgatgcgct ccatgaacaa tggacagata tcaaggcctg tccggttcac
     5941 tttgtggaac ggctcatgca ttacttcctg acttacaaag atgctcccgg ctcacaaaaa
     6001 aagaattgcg aaatcaccca tgtgtatggc cgcgaggaag cccacgaggt cattcgccgc
     6061 agtcaacagg attacgccgc gaaatatgga cacctccaag cccaacttgc caaaccccgc
     6121 catgcactta attaaaaacg cagcccctct gaacttctct catcgatctc aatgccattt
     6181 gaaataaaag tcccaacggc ttgcggtcag cgacggttct cctgttcata gcct
//
LOCUS       DATIAL010000285         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1235062, whole genome shotgun sequence.
ACCESSION   DATIAL010000285 DATIAL010000000
VERSION     DATIAL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235062"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VLA60_08810"
     CDS             <1..726
                     /locus_tag="VLA60_08810"
                     /inference="COORDINATES: protein motif:HMM:NF017119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HSF09502.1"
                     /translation="REMPGMGSLVTQKQDWPSPVMDEQHHGFFLADVLEYRPNIGGDG
                     SHDDYRWDIEGWYGGDYNRIWFKSEGQRDTAFKADYDIDSQLLFGRFIQQYYDFQVGG
                     RVETQSYRGRNVTRGLAVIGLQGLVPYNYEIQPALFISQHGDLSARLTATKDLSLTQR
                     LILQPRFETNAAIQRVRKFTTGSGLNNLELGFRLRYEIRREFAPYVGISLDKSFGDTT
                     SLVRKEGGNPSQIRFVVGVRLWF"
     gene            1017..1376
                     /locus_tag="VLA60_08815"
     CDS             1017..1376
                     /locus_tag="VLA60_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09503.1"
                     /translation="MNKGFPKKARVVVPVFVIVSLVLCGVAGAYCPMSSSAAASHSSQ
                     PASPHSPSDHTKECPEQLSTAKEQLKELTPVALPVTHFHILDLFTNASTQKLLFARTT
                     PSSSYPLLFLHFSVLLN"
     gene            1474..2832
                     /locus_tag="VLA60_08820"
     CDS             1474..2832
                     /locus_tag="VLA60_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF09504.1"
                     /translation="MNSFPFYGVHVRVLLVSTSLLLACMATGPAFAQESPLTLHTLVE
                     EALLRNPDIQAANRRWEASQAIIPQVQSLPDPNLLLGYQRMPMLEPLEGPVYGFSQKI
                     PFPGKLGLKGEVAARQADRIEQEFLATQLNVVARLKRHYWDLHFIHKSIEIVEKNKLL
                     LMQFEKTANARYSVGKTAQQDVFRAQVELSRVLDRLAVLEQQKESLHAAINRILNRPP
                     IGPLGTPEEIHLTVIPYDLPELARRVDAFSPILLASAKSVAQGEESVSLARKEYFPDF
                     NLSALGTRNERINENGYQIMFGIQIPLFYQTKQREGVNQAAADLSRAREDLTATRQDL
                     LFQVKDAFERAQRATRLVNIVGKAIIPQATLALQSAQAGYSVDKVDFLTLLNNLLTLQ
                     ENELELHGEMVEHEKAVARLEELTGGPLTVSSPTGSQEEYQGLHQSQSAPNHDRSGTT
                     GP"
     gene            2829..4061
                     /locus_tag="VLA60_08825"
     CDS             2829..4061
                     /locus_tag="VLA60_08825"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF09505.1"
                     /translation="MKQWTFYLGIGVLIVGGLIGTYLFLNREPARVSPGPSEKTTAQG
                     PAGQDVKQRPPHEHQGSGPASEPQTPPMPNTLTIAPERLQSIGVRFEEATRRSLARTI
                     RTVGRVEIDERRLARVNIKFEGWIEDLRVSAIGDHVKQHQILFTIYSPDLVATQEEYL
                     LALQGIRELGTSEFPVVAKGAKDLLEATRRRFQLWDITENHIQDLERTGEVLRTLPIH
                     SPITGTVLKMEARAGTHVTPGTELYMIADLSRIWIMADIYEYELPLIQLDQKATVTLS
                     YDPRTHLVGSVGFIYPTLDPQTRTAKVRFEVDNPGEKLKPGMYANVELTIPLGRRLAI
                     PRAAVLETGERQIVFIHQGGGTLEWRDAKLGVQTGEWVEVLKGVQEGDHIVTSANFLI
                     DSESQLKSAVRGMAGMKH"
BASE COUNT         1019 a         1112 c         1049 g          933 t
ORIGIN      
        1 agagaaatgc cgggcatggg atcacttgta acccaaaaac aggactggcc cagtcctgtc
       61 atggatgagc agcatcacgg ttttttcctc gccgacgtcc ttgaataccg gccgaacatt
      121 ggcggggacg gaagtcatga cgattaccgg tgggacatcg agggctggta cggcggtgac
      181 tataatcgaa tctggttcaa aagcgagggc cagcgggaca cggcatttaa ggccgactac
      241 gacattgatt cacaactcct tttcggacgc ttcattcagc agtattacga ttttcaagta
      301 ggggggcgcg tcgaaacaca atcctatcgg ggacggaacg tcacccgggg gcttgcggtg
      361 atcgggctac aaggtctcgt cccgtataac tatgaaattc agcccgccct cttcattagt
      421 caacatggag atctgtccgc acgattgacc gcgaccaaag atctctccct cacgcaacgt
      481 ctgatcctcc agcctcgctt tgagacgaat gccgcgattc aacgcgtccg gaagttcaca
      541 acgggttcag gactgaataa ccttgaattg gggtttcggc tgcgttatga aatccgacgg
      601 gaatttgccc cctacgtggg aatctccttg gacaaaagtt ttggagatac cacctcgctt
      661 gtccgaaaag aaggaggaaa tcccagtcag atacgattcg tggtgggcgt aaggctctgg
      721 ttttaacaca agccctgctc gcacactggt ggcaaggtgg aatatgtcct cgttccatcc
      781 ctcacgagat ggtgtagcag ctcaactata aaaaagtgtt gcatttaggg acttctagca
      841 attcaccctt tgggtagaat tgcaacacat tgttttaatc tttggacaat gctaatccaa
      901 aaaaagcgtt taaaatgaac atgggctttt gtgtggcatt acgtttgctt ttctaaagag
      961 gtgcctaaac ctcacacaga agctgtcgga taatgtcaaa agaaaaagct cggccaatga
     1021 ataaaggctt tccaaagaag gcccgagttg ttgttcccgt cttcgtgatc gtgtccttgg
     1081 tcctatgcgg ggtagcgggt gcatactgtc cgatgtcctc ttctgcagcg gccagtcact
     1141 cttctcagcc cgcatccccc cattctccat cggaccacac caaggaatgc ccggaacaac
     1201 tcagcactgc caaggagcaa ttgaaggaat taactcctgt cgccttgccc gtcacacatt
     1261 tccacattct tgatctcttt accaatgcct ctactcagaa attgctgttt gcacgcacga
     1321 caccatcctc atcctatccg ctcctcttcc tgcatttttc tgttcttctg aattgaattt
     1381 tcctctccaa ctgtcggcat acgtatccgg aagccatcag ggttttcgga acgttctggt
     1441 tttttttact ctctcttgtc gtgagggggc tttatgaact catttccatt ctatggtgtg
     1501 catgtacgtg tgctgttagt tagcaccagc ctgctattgg cctgtatggc caccggacct
     1561 gccttcgccc aagaatcccc ccttaccctc cacacccttg tagaagaagc tcttctacgc
     1621 aatccggaca ttcaagcagc caatcggcgg tgggaagctt cgcaagccat cattccacag
     1681 gtgcaatccc tgccggaccc gaacctgctc ttgggatacc agcgaatgcc catgctcgag
     1741 ccgctggaag gcccggtcta cggattcagt cagaaaatcc cctttcccgg gaaactcggg
     1801 ctgaaaggag aagtcgccgc ccggcaggcc gaccggattg aacaggagtt tctggctaca
     1861 caactcaacg tcgttgcccg cctgaaacga cattattggg atctccactt catccataag
     1921 agtatagaaa ttgtcgagaa gaacaaattg ctcctcatgc aattcgagaa gaccgccaac
     1981 gcccggtaca gtgtcggaaa aacggcgcag caggatgtat ttcgcgccca ggtggaactc
     2041 tcgcgggtgt tggatcgact ggcggtgttg gaacaacaaa aagaaagtct gcatgccgcc
     2101 ataaaccgaa tactcaaccg tcccccaatc ggaccacttg gaaccccgga agagattcat
     2161 ctcacggtta tcccgtatga cctgccggag ctggcgagac gcgttgatgc cttctcgcca
     2221 attcttctgg cgtccgccaa aagcgtggcg caaggggagg aatcggtctc cctggcccgg
     2281 aaggaatatt ttccggattt caacctgagc gcgttgggca ctcggaatga gcggataaac
     2341 gaaaatggat accagatcat gttcggtata caaattccac tgttttatca aacgaagcag
     2401 agggaaggtg tcaatcaggc ggcggcggat ctttctcgag cacgcgagga tctcaccgcc
     2461 acccgccaag atttgttgtt tcaggtgaag gatgcgtttg aacgggctca acgagcaacc
     2521 cgactggtca atattgtcgg gaaggccatc attcctcaag ccaccctcgc cctccaatcc
     2581 gcccaggcag ggtatagcgt cgataaagtg gactttctca ctcttctgaa taatctgctg
     2641 accctccagg aaaatgagtt ggagcttcat ggggagatgg tggaacatga aaaggccgtc
     2701 gcgagactag aagaactgac cggagggccg ctaacggtct cgagtcctac aggttcccag
     2761 gaagagtatc aaggcttgca tcaaagccaa tccgcaccaa accacgatcg atcggggacg
     2821 actggaccat gaaacagtgg acgttctatc tcggaattgg tgtgctgatt gtcggtggcc
     2881 tgatcgggac ttacttgttc ctcaaccgag agcctgcgcg tgtttctccc gggccttcgg
     2941 aaaagacaac cgcccaaggg cctgccgggc aggatgtgaa acaacgacca ccacatgaac
     3001 atcagggttc aggacctgct tccgaaccac agacaccacc catgcccaat accctcacca
     3061 ttgcccctga acgattgcaa tcaatcggcg tgcggtttga ggaggcaacc cgtcgatcgc
     3121 tggcccgaac cattcgaacc gtgggacggg ttgaaatcga cgaacgccgc ttggcgcggg
     3181 taaatattaa atttgaggga tggattgagg atttgcgcgt gagcgccatc ggggatcatg
     3241 tcaaacagca ccagatcctt tttacgattt acagtccgga ccttgtcgcg actcaagaag
     3301 agtacctctt ggcgcttcaa ggaattcggg aactcggtac aagcgaattt cccgtagttg
     3361 ccaagggcgc caaggatctg cttgaggcca cacgacggcg gtttcaactg tgggatatca
     3421 cggaaaatca tattcaggac cttgaacgaa ccggggaagt gttgaggaca ttacccattc
     3481 actccccgat cacaggaacc gttctcaaga tggaagcccg cgccggaacg catgtgaccc
     3541 caggcaccga actctacatg attgcggatt tgtctcggat ttggatcatg gcggatattt
     3601 acgaatacga attgcccttg atccagctcg accaaaaagc caccgtcacg ctctcctatg
     3661 atcctcggac acacctggtc ggatcggtgg gattcatcta tccgaccttg gatcctcaaa
     3721 cccgtacagc caaagttcgg ttcgaagtcg acaatccggg cgaaaaactg aaacccggca
     3781 tgtatgccaa tgtggaactc accattccct tgggacgccg gctggccatt ccgcgtgctg
     3841 ccgtgcttga aaccggagaa cgccaaatcg tatttatcca tcagggagga ggcacgctgg
     3901 aatggcgaga tgcgaagctt ggcgtacaga ccggagaatg ggtggaagtc ctgaaaggag
     3961 tccaagaagg agatcacatc gtgacatcag ccaacttttt gattgactcc gagagccaat
     4021 tgaagtccgc cgtccgcggc atggccggta tgaaacatta aaaaatagat tcatcatggt
     4081 tgcccacatc attgaattca gcgcccggaa tcg
//
LOCUS       DATIAL010000286         5534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_53235, whole genome shotgun sequence.
ACCESSION   DATIAL010000286 DATIAL010000000
VERSION     DATIAL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53235"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VLA60_08830"
     CDS             complement(<1..1324)
                     /locus_tag="VLA60_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF09506.1"
                     /translation="MPNSVQDWIDALEDADDATREEAAKALAEKGDPKTLEPLLIALE
                     DDYWSVRTYVGWALAKIGGEKAVEGLITLFNDNMMEVQAEAVKAMASMGKCVIPQLLT
                     GLKDKRWRVREQAAKTLGELRDPQAVPGLNLVCRDRDGAVKSAAAEALGKIGDPKAIP
                     TLIKLFKDTSKIVRETAGTALMYIGKESVDALLESLKDPHFVVRCHAVRALGGMTTDY
                     QMGRVWVREARVVDALIDMVKDPDRAVREDATIALGNIGDARAVDALMEAMKDGTVKR
                     HAIASLGMIGDPRAYPPVLDALKGKGIHQEGKPTPGCIISEDLLIKEAAATALGHFRH
                     PKVIPDLVLLLKDLVLRDYAANSLVLIGDAAIEPLVWFLHDPDLSKVQQESERVLAFA
                     TTRMTAGGALKKTVLDTLDKLGWKPTEGLSKETREIEYTDTSNVLGEGG"
     gene            complement(1350..1823)
                     /locus_tag="VLA60_08835"
     CDS             complement(1350..1823)
                     /locus_tag="VLA60_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09507.1"
                     /translation="MNHKQNNKTMIRTHVPRQIRRGIFCLFLMGISPLLVHHGHTADP
                     INPLLREFPITVNPNALTPPTWWHVPGITPLIWTKDPINMDAYKTSESRTLNLKPGDY
                     RFGTFTFDFLFKVTLSGTLEFSPTLDQCVDGRGTQVLTIRCSHTQPYPQEPDYPK"
     gene            complement(1801..2856)
                     /locus_tag="VLA60_08840"
     CDS             complement(1801..2856)
                     /locus_tag="VLA60_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HSF09508.1"
                     /translation="MSTSQLRPQQIRRGKLIRCVFLAWSMAWLPLIGGTASIASAAQA
                     EHIILVVLEGIKSSSIQSGATPNLAQLAKDGAVTWSAQSITPPLTVPAMASLLTGLQV
                     EKHRVTAAWETYDFARSFMRSPTVFDYMDLAGGKDTGVFLMDERLYQLVRPEIYVDSQ
                     VCGYTKPQCTPERASAYIKDFLKKVTSEKGYGFRLFGVPNLLLVHLPTAVKVGQKYGW
                     DSEKYSAALLSIDTTVEQIIQTYKELGVFDKTMVIITGLNSGPLAPPTGGGKTVSTSL
                     TTPELDATIPWIAWGANIKRNHPITQSVSLLDTGATIMYALGLETHTEWDSHVIGDIF
                     QAVPERRTTDNEPQTKQ"
     gene            3120..4394
                     /locus_tag="VLA60_08845"
     CDS             3120..4394
                     /locus_tag="VLA60_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09509.1"
                     /translation="MPHENNFQHANYLKSDPGVRVGYRTFQPGTCSDSPSWGQAMGGV
                     GERMAQFRVKGILFLNGLPFMDLFGAARLDEVGGLKRGYSRGISGLESLLALLRPTTN
                     GICLPDDPIHPPVANDEPTHGRVDLLAQEAGNFSSSYVRKFELALTQGSGQSIPCGRY
                     LWSSINHHVGRVEAAMHLLVYLQDWVSRLDLTRDDRLLLVGHGHAGQVLALLSNILAR
                     GESEMRGRVFEILAKYWQACPSADRSVEQLERLYGLVMDQTVLKGATVDVVTLGTPVR
                     YGWDTDGIGHLLHFVNHREIRTDGKRWLAKMELPQIAWEMPYQTGGDYVQQLAVAGTD
                     MVPNNPEAEQANVNFREIFEPYDGFERWLECTRRGTRCANDGQCLLVEYGVQAEESPR
                     KHLFGHACYTQSRAMLFLATEIVQAFYSPKSH"
     gene            4658..>5534
                     /locus_tag="VLA60_08850"
     CDS             4658..>5534
                     /locus_tag="VLA60_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09510.1"
                     /translation="MIVGVLRETYPGEHRVALVPAVLPSLKKGGMDVMVESKAGEQAG
                     YPDSAYVEKGATIVSSRAQVFEKADCVVQVRLLGANLTEGQADLTGFRKGQMLVGMAE
                     ALTAPQSVQDLASREVMAFALELMPRITRAQSMDILSSMGTVAGYKAVLIAASSLPKM
                     FPMLMTAAGTVTPAKVLIIGAGVAGLQAISVAKRLGAAVEAYDIRPAVKEQILSLGAK
                     FVELPLETGAAEDKGGYAKAQDETFYRKQRELLGKVIATSDVVISTAAVPGKKAPILI
                     TADMVGAMAPGSVIVD"
BASE COUNT         1302 a         1299 c         1456 g         1477 t
ORIGIN      
        1 ctcctccctc acctaacaca ttggaggtgt cggtatattc aatctctctg gtttctttgc
       61 tgaggccttc agttggtttc catccgagtt tatccagggt gtctaagact gtctttttaa
      121 gggcaccacc tgccgtcatt cgagtggtcg caaaagccaa aacccgctcg ctttcttgct
      181 ggactttaga caaatccggg tcatggagga accatacgag tggctcaatc gctgcgtctc
      241 caatcaacac caaggaattg gcggcataat cacgaaggac caggtctttg agtaacagca
      301 ctaaatccgg aatgactttg ggatggcgga aatgaccgag tgctgtcgca gccgcttctt
      361 taattaagag gtcttcgcta ataatgcatc caggggttgg cttcccctct tgatgtatgc
      421 ccttaccctt taacgcgtcc aggacgggag gataggcgcg aggatctccg atcatgccga
      481 gggaagcaat ggcatggcgt ttcactgtac cgtctttcat ggcttccatg agagcatcta
      541 ctgcacgggc atcgccgata ttgcccaagg cgatagtggc atcttcgcga accgctcgat
      601 cggggtcttt taccatgtcg atcagggcat cgacgacacg agcttcacga acccacaccc
      661 gccccatttg gtagtctgtc gtcatgcccc ctagtgcccg aacggcgtga cagcgcacaa
      721 cgaagtgagg atctttgagg gactccagta gggcatcgac tgattctttc ccaatgtaca
      781 tcaatgcggt tccggcggtt tcccgcacga tcttggatgt atctttaaat aatttaatca
      841 gagttggaat ggcctttggg tctccaattt ttcctaaggc ttcggctgcg gcactcttga
      901 ctgcaccatc ccggtcgcga cacaccaggt tcaatcctgg tacggcctgc gggtcacgca
      961 attcacctaa cgttttagcg gcttgttctc gcacccgcca ccgcttgtct ttcaagccag
     1021 tcagtaattg cggaatgacg cattttccca tggaggccat ggcttttact gcttcagctt
     1081 gcacttccat catattgtca ttaaaaagcg taatcagacc ttcaacggcc ttttctccgc
     1141 caattttggc taacgcccag ccgacataag ttcgaaccga ccaatagtcg tcttcaaggg
     1201 cgatcagaag gggttctaac gttttcggat cacccttttc agcaagggcc ttggcggcct
     1261 cttcgcgagt ggcatcatcg gcatcttcga gggcatcaat ccagtcttgc acagaattcg
     1321 gcatgatcat ccttcttatc ccttccgact tatttcgggt agtcgggttc ttgtggatag
     1381 ggttgcgtat ggctacaacg aattgtcagg acctgtgttc ctcgtccatc tacacattga
     1441 tcaagtgttg gcgagaattc caaggttccg gataaggtca ccttgaataa aaaatcaaac
     1501 gtaaaggtcc caaaccgata atctccaggc ttgaggttta aggtcctgga ttccgaggtt
     1561 ttgtatgcgt ccatattgat gggatctttt gtccaaatca acggagtgat gcccggtacg
     1621 tgccaccagg ttgggggcgt gagtgcattc ggattcacgg taataggaaa ctctcgcagc
     1681 agaggattga taggatctgc tgtgtgcccg tggtgaacaa gcagaggaga gatgcccatc
     1741 agaaacaggc agaaaatccc tcgtcggatt tgacgaggca cgtgtgttcg aatcatggtc
     1801 ttattgtttt gtttgtggtt cattgtcggt ggtccgtctt tctggaacag cttggaaaat
     1861 atcaccaatc acgtggctgt cccattcagt atgcgtttca agccccaggg catacataat
     1921 agtcgctccg gtatctaata gagagaccga ttgggtgatg ggatggttcc tttttatatt
     1981 cgcgccccag gcaatccagg gaatggtggc atctagctct ggggtcgtaa ggctggtcga
     2041 gactgttttg cctccccctg tcgggggtgc aagaggcccg ctattcaggc cagtaataat
     2101 caccatggtt ttgtcaaaaa cgccaagttc tttatacgtt tggatgattt gttccacggt
     2161 ggtatcaatg gacagtaagg cagcggaata tttttcggaa tcccacccgt atttctgccc
     2221 gaccttcacc gccgtaggca gatgtaccaa taagagattt ggtaccccaa aaagccggaa
     2281 gccatagcct ttctcactgg tgactttttt taaaaagtct ttgatatatg ccgacgctct
     2341 ttcaggcgtg cattggggtt tggtgtagcc acaaacttgg gagtcgacgt aaatttccgg
     2401 tcgaactaat tgatagagtc gttcatccat aagaaagacg cccgtatctt ttcctccagc
     2461 cagatccatg tagtcaaaga ccgtgggtga gcgcataaat gaacgtgcaa aatcgtacgt
     2521 ttcccatgcg gcggtcaccc gatgcttctc gacttgaagg ccggtgagca gggaggccat
     2581 cgccggaacg gtgagaggag gggtaatgga ttgagcagac caggtcacag ctccatcctt
     2641 ggctaactga gccaggttcg gagtcgcgcc gctttggatg gatgatgatt tgatcccctc
     2701 cagcacgacc agaataatgt gttcggcttg tgcggcggaa gcaatcgagg ctgttcctcc
     2761 gattagcgga agccaggcca tactccatgc taggaaaaca caccgaatga gtttgccacg
     2821 acgaatttgt tgaggacgta gttgggaagt actcataatt tctgccctgc tccacgacaa
     2881 gaaaataaag atgaaattgg gaacgaacct ttattatata tcattaggag aattctccct
     2941 cacttgtgag gaaagtaata gaaactgaac ttatcatgca gattttcagg cggtcaacca
     3001 actgagaagg ccaggatttt cattccatta gaatcggcag accaaaaatt ttcaaggatc
     3061 tcttgccctg ggtggagggt atccattatg aatggggcct cacacataga tgatttccaa
     3121 tgccccacga aaataatttc caacatgcta attatttaaa gtcggaccct ggagtacggg
     3181 taggctatcg aacctttcag cctggaacgt gttccgattc gccttcatgg ggccaggcaa
     3241 tgggtggcgt tggcgaacgg atggcccaat tccgagtgaa gggaatcctc tttctcaatg
     3301 gcctgccatt tatggatctc tttggtgcgg ccagattgga tgaggttgga gggttaaaac
     3361 gaggatattc ccgaggaatt tctggcttgg aatcgttgct ggctctcctt cgccctacta
     3421 caaacggtat ttgtcttcca gatgatccca ttcaccctcc tgtagcgaac gatgaaccaa
     3481 ctcacgggag ggtagacctg ctcgctcagg aagcaggaaa tttttcttcg tcgtatgtac
     3541 ggaaatttga actcgccctg actcagggga gcggccaatc tattccctgt ggtcgatacc
     3601 tttggtcttc gattaatcat catgttggcc gcgtcgaagc cgccatgcat cttttggtgt
     3661 atcttcagga ttgggtttct aggcttgatc ttacaagaga tgaccggctt cttctcgttg
     3721 gccatggaca tgcgggtcag gttctggctt tattgtcgaa tattctagct agaggggaat
     3781 cggaaatgag ggggcgcgtt tttgagattt tagccaaata ctggcaagcg tgcccttcag
     3841 ccgatcggtc cgtagagcag ctcgaacgct tgtatggtct tgtcatggat caaacggtgt
     3901 taaaaggcgc aacggtcgat gtcgtcacgt tgggaactcc ggttcgctat ggctgggata
     3961 ccgatggaat tggtcatctg ctgcatttcg tgaatcatcg tgagattcga actgatggca
     4021 agcgttggtt ggcgaaaatg gaattacctc aaattgcgtg ggaaatgccc tatcaaactg
     4081 gtggggatta cgtacagcaa ttggctgtgg ctggcacgga tatggttccg aataatccgg
     4141 aggctgagca ggccaatgtt aatttcagag aaatttttga accctatgac ggctttgagc
     4201 ggtggctgga atgtactcgg cgtgggacac ggtgtgccaa tgacggacaa tgtctgttgg
     4261 tggaatatgg agttcaggcg gaggagtctc ctcgaaagca cttatttggc catgcatgtt
     4321 atacccagag ccgggctatg ttatttctag cgactgaaat cgtacaggca ttctattcac
     4381 cgaagagtca ttgattgaat gaaagaatca ttttggtccc atatgcagtg atgatattct
     4441 gggatttttc attcgatgtg ccatgagccg tgttgcctct gtttcgcctt tcgcgctggg
     4501 ccgttagtcg cacttcctta tagatccaaa gacacattga ttctctttga atccctatga
     4561 tatcaacaca gggcttgcca aggggtttaa aaaatccctg cagacttaat gaaattgtaa
     4621 agttcctaca acccttatat ctaagagata ggcccttatg attgttggcg tccttcgaga
     4681 aacgtatcct ggtgaacacc gagtggctct agttccagct gtacttccct ctctgaaaaa
     4741 agggggcatg gatgtcatgg tggaatccaa ggctggagaa caagcgggtt atcctgactc
     4801 cgcgtatgtc gaaaaaggcg caaccattgt ctcatctcgg gcacaagtgt tcgaaaaggc
     4861 tgattgcgtt gttcaagtgc ggttgttggg agctaaccta accgaaggtc aagcagactt
     4921 aacaggcttt cgcaaagggc agatgctggt tggaatggcc gaagccttga ctgccccgca
     4981 atcggttcaa gaccttgctt ctcgtgaggt catggccttt gctttagaat tgatgcctcg
     5041 cattacccgt gcccaaagta tggacatcct ttcctccatg ggcacggtgg ctggctacaa
     5101 agcggtactg attgcggcca gttcgctgcc aaaaatgttt ccaatgctca tgacagcggc
     5161 cggaacggtc actcctgcga aggtcttaat cattggcgct ggggttgccg gattgcaagc
     5221 catttctgtg gctaagcggt taggagctgc tgttgaggcg tatgatattc gccctgcggt
     5281 gaaagagcag attttaagtt taggcgccaa gtttgttgag ctgccactgg agacaggtgc
     5341 tgcggaagat aaaggggggt atgccaaagc ccaagatgaa accttttaca ggaaacagcg
     5401 agagttatta gggaaagtaa ttgccaccag tgatgtggtg atttccacgg cggctgtgcc
     5461 tgggaaaaaa gcaccgattc ttattacggc cgatatggtt ggggctatgg ctccagggtc
     5521 agtaattgta gatt
//
LOCUS       DATIAL010000287         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_483097, whole genome shotgun sequence.
ACCESSION   DATIAL010000287 DATIAL010000000
VERSION     DATIAL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483097"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>3348
                     /locus_tag="VLA60_08855"
     CDS             13..>3348
                     /locus_tag="VLA60_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HSF09511.1"
                     /translation="MSESTTDRQPNRLSHETSPYLLQHAYNPVDWYPWGEEALSRAKQ
                     ENRPILLSIGYSACHWCHVMERESFENQAIADLMNRYFVNIKVDREERPDLDEIYMQA
                     TTAMNQGHGGWPMTVFLTPDQEPIFAGTYFPPTDRYGRPGFGSILRNIGEGWLRDQDN
                     IVQQAGRFTTRLRAALQPASPLAVGAAEIEDAVRQFARDFDARYGGFGRAPKFPPATG
                     LSFLLRQYHWSQEEKILAMVTKTLDGMAAGGLYDHIGGGFARYSTDEEWLVPHFEKML
                     YDNALLARIYVEAFQVTGKNQYRQVATETLDYILREMTSPEGGFYSATDADSEGEEGK
                     FFVWTPQQIRDIVTNGLDATCFCAYYDITDEGNWEHTNIPRTPRSPELVAQELGCSPE
                     DLHDSVNRVKPIIYQARLNRVPPGLDDKIITAWNGMMIGTMAEAGRVFNHQPYLDGAI
                     RAADFLLTTLSRPESRLWRTYRAGHAHLNACLEDYAYAAEAMIDVYEATAYERYLREA
                     VSLAERILEDFAARDHGGFFTTAADHEALIIRGREGADGATPSGNAVAASALARLSFH
                     RDREDFRIAATNAIRAYGEQIGQVPRGFPKSLMVIDFLLRGPVEMALVGIPGDKEYNQ
                     LRSAVNTCFVPYRIVAYRQELESESSHPLLAGKTLVNGKAALYICKNFACQVPLTDPQ
                     SVPAALSHPQEISPSSEGEPLQALTSHGIPGKATPQGTGTYVARILASPRDSRPSSHG
                     YASLGSTGLTTSRIGFGGYRIDVGVEDHRRALEKALREGCNLIDTSTNYADGGSERLV
                     GVVLKDLMANHDILREEVIIISKIGYVQGTNLTRAEAREQTGKPFPEMIKYGDGIWHC
                     LHPVFLAEQLILSLDRLGLETLDVCLLHNPEYFLSDAKNRQLSTDPIRLEDLRQEFYR
                     RLQKAFAYLESQIAAGRLQYYGVSSNTCTAKPDNPEATSLSRMLKAAEAAAKQAGIPH
                     HHFRVLQLPMNLFESGALLTPNTGPEESQTVLAMAREANIAVLVNRPLNAILEKSQGM
                     IRLADPQVEISNTNFDAQQSKVAALEHDYKQVLAPQIPKPEKGAAPLDYFNWAEELKR
                     IRSSIQNLEHWDQIESQTIAPHAN"
BASE COUNT          830 a          923 c          849 g          746 t
ORIGIN      
        1 acggtccctt ttatgtctga atccacaact gatcgccaac ccaatcgact gagtcacgaa
       61 accagtccct atctcctgca acatgcttac aatccggtgg attggtaccc atggggcgaa
      121 gaggcgctta gccgggctaa gcaagagaac aggcctattt tactttccat cggatattcc
      181 gcctgccatt ggtgccatgt gatggaacgg gaatcgtttg aaaatcaagc catcgcggat
      241 ctcatgaacc gctacttcgt gaatattaaa gtggacaggg aagaacgtcc ggatttggat
      301 gaaatttaca tgcaggccac gaccgctatg aaccaggggc acggcggctg gccgatgacg
      361 gtatttctca cacccgatca ggaaccaatt tttgcaggaa cgtattttcc tcccactgac
      421 cgctatggcc gtccgggatt cggatccatt ctacgaaaca ttggcgaggg gtggcttagg
      481 gatcaagaca atattgtgca acaagccggt cgattcacca cacgtttacg ggctgcctta
      541 caaccggcct ccccgcttgc ggtgggtgca gccgagatcg aagatgccgt gaggcaattt
      601 gcgcgggatt tcgatgctcg gtatggagga tttggacgcg cccctaaatt tccacctgcc
      661 accgggctct cctttctttt gcgccaatat cactggagtc aggaagaaaa aattcttgcc
      721 atggtgacca aaaccctcga tggcatggcc gcaggcggtt tgtacgatca catcggtgga
      781 gggtttgccc ggtattccac cgatgaagaa tggttggtcc cgcatttcga aaaaatgcta
      841 tatgacaacg ctctacttgc acggatctat gttgaggcct ttcaggtcac aggaaaaaat
      901 cagtatcgcc aggtcgcgac cgagactttg gattatattt tgcgtgaaat gacctcaccg
      961 gaaggcgggt tttattcggc cactgacgcc gatagcgaag gagaggaagg caaatttttc
     1021 gtgtggacgc cgcaacaaat tcgtgacatc gtcaccaacg gtctggacgc cacttgcttc
     1081 tgcgcctatt acgacatcac ggatgaaggc aattgggaac acaccaacat accacgcaca
     1141 cctagatcac ctgagctggt ggcacaggaa ttggggtgtt cccctgaaga tctacatgac
     1201 tccgttaaca gggtaaaacc gatcatatat caggctcgcc tgaaccgggt gccgccgggc
     1261 ctcgatgata aaatcattac ggcctggaat ggcatgatga tcggaactat ggccgaagcc
     1321 ggtcgggtgt tcaaccacca accctatctg gatggcgcca tccgggccgc cgattttctg
     1381 ctgaccactc tttcccgtcc agaaagccgg ttgtggcgca cctatcgtgc ggggcatgcc
     1441 catctcaatg cctgcttgga ggattatgcc tacgcggctg aagccatgat cgatgtctat
     1501 gaagccacgg catacgaacg gtatctccgt gaagccgttt ccttggctga acgcattctg
     1561 gaagattttg cagctcggga tcatggagga ttctttacaa ctgccgccga ccatgaagcc
     1621 ctaattatcc ggggacgaga aggagccgac ggggcaacgc ccagcggaaa tgccgtagct
     1681 gcctcggctt tggcccgctt atcgtttcat cgtgatcgtg aagactttcg aatcgctgcg
     1741 accaatgcca ttagggccta tggagaacaa atcgggcagg tgcccagagg gtttcccaaa
     1801 agtctcatgg tcatcgattt tttattacga ggtcctgtgg aaatggcttt agtgggaatt
     1861 ccaggggaca aggaatataa ccagctccgc tcggcggtca atacctgttt tgtgccttac
     1921 cggattgtag cctaccggca ggagctggag agtgaatcat ctcatcctct gttagcagga
     1981 aaaaccctgg tcaatgggaa ggccgctctt tatatctgta aaaacttcgc atgtcaggtc
     2041 ccgctcaccg acccccaatc cgttccggcc gcgttgagcc atccccagga aatttccccc
     2101 tcatcggagg gagaaccatt gcaggctctg accagtcatg gtatccctgg aaaggccact
     2161 ccccaaggaa cgggaacata tgtggccaga atattagcca gtccacgcga ttcccgccca
     2221 tcttcccatg gctacgcgtc tcttggttca accggcctga ccacttctcg aatcggcttc
     2281 ggtggatacc ggatagacgt aggagtggaa gaccatcgac gggcccttga aaaagcctta
     2341 cgggaggggt gcaatctgat cgatacatcc accaattatg ccgatggtgg cagcgaacgt
     2401 ttggtcggtg tggttctcaa ggatctcatg gccaaccacg atattttgcg tgaggaagtt
     2461 attataattt caaaaatcgg ctatgtgcag ggaaccaacc tcacccgcgc agaagcaaga
     2521 gaacaaaccg gcaaaccctt tccagagatg atcaaatatg gcgacggcat ctggcattgc
     2581 ctccatccgg tatttttagc agagcaactc atcctttcgc tcgaccggct gggactggaa
     2641 accctggatg tctgcctctt gcacaacccc gaatattttc tgtcggatgc caaaaatcgc
     2701 caattatcaa ccgaccctat caggctggaa gatctccgtc aggaatttta tcggcgtctg
     2761 caaaaggctt ttgcctatct ggaatcacaa atcgccgccg gccggttgca atattatggc
     2821 gtctcctcca atacctgcac ggccaaaccc gataatcccg aagccacgtc gctgtctcga
     2881 atgctcaagg ccgcagaggc tgccgcgaag caggccggga ttcctcacca tcattttcgt
     2941 gtcctccaac tcccgatgaa tttatttgaa tcgggagccc tactcacacc caataccggc
     3001 cccgaagagt cacaaacggt gttggcaatg gcccgggaag ccaacatcgc cgtattggtc
     3061 aaccggccac tgaatgccat cctggaaaaa agccaaggta tgatccgcct cgcagacccg
     3121 caggtggaaa tctccaatac gaactttgat gcccagcaat caaaagtcgc cgccctggaa
     3181 cacgactaca agcaggtgtt agcaccccag atcccaaaac cggaaaaggg agctgctcca
     3241 ctggattatt tcaactgggc cgaagaactg aaacgaattc gatcctccat acaaaatctg
     3301 gagcactggg accaaatcga atcccaaacg attgccccac acgccaat
//
LOCUS       DATIAL010000288        12431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1451896, whole genome shotgun sequence.
ACCESSION   DATIAL010000288 DATIAL010000000
VERSION     DATIAL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451896"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..560
                     /locus_tag="VLA60_08860"
     CDS             135..560
                     /locus_tag="VLA60_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09512.1"
                     /translation="MQKHWDVMRSDDSGESWHEISGDLPSDFGFPIAVHAHEPETIYV
                     VPIKSDSEHYPPEGKLRVYRSRTGGNEWEALTKGLPQQDCYVNVLRDAMAIDSLEPCG
                     IYVGTTGGQVYASADSGDSWMPIVRDLPPVLSVEVQALP"
     gene            557..859
                     /locus_tag="VLA60_08865"
     CDS             557..859
                     /locus_tag="VLA60_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09513.1"
                     /translation="MSTIRVVLPAHLRTLARIDGEVNLEMEGPVTPHAILNALETRYP
                     MLRGTIRDQVTQQRRPFIRFFACGQDLSHEPPDALLPDAITTGEEPFLIVGAMAGG"
     gene            complement(926..1675)
                     /locus_tag="VLA60_08870"
     CDS             complement(926..1675)
                     /locus_tag="VLA60_08870"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSF09514.1"
                     /translation="MNGSGQSTAPVALPRTMLERANAIDWGQVSSDLDAQESAVTEGL
                     LTSKECEALAMLYSRAEIFRSRIVMSRHNFGRGEYQYFRYPLPDLIEQLRNAMYPHLV
                     PVANRWNAAMGIEIRFPKRHEDFLARCHHSGQDKPTPLMLKYETDDYNCLHQDLYGEH
                     VFPLQLAILLSDPQKDFTGGEFVMTEQRPRMQSRPIVIPMRQGDGVIFAVHHRPVRGG
                     RGWYRVNLRHGVSRVRSGQRYTVGIIFHDAK"
     gene            complement(1672..2751)
                     /gene="ada"
                     /locus_tag="VLA60_08875"
     CDS             complement(1672..2751)
                     /gene="ada"
                     /locus_tag="VLA60_08875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HSF09515.1"
                     /translation="MRNTANQETCSVTTRHDPRWTIVVSRDPTADGTFFYSVNTTGVY
                     CRPSCPSRLPRPEHVRFHASREDAEKAGFRPCKRCTPNLPGLQERYAEKVAAACRFIE
                     DSDHAPGLKELSHHAGLSRYHFHRVFKAVTGLTPKGYTAAHLAKRVRSHLNHKGTVTE
                     AIYEAGFNSNGRFYETSDAALGMTPSSYRAGGSGIDIRFAVGECSLGSILVARSERGV
                     CAILLGDDPEQLTRDLQKQFSQAHLIGGDGDFERLVATVIGFVEAPGRGLDLPLDVQG
                     TAFQQRVWKALQNIPAGSTVSYADIAASIGAPKAIRAVASACGANAIAVAIPCHRVVR
                     TNGELSGYRWGIERKRALLEREAHT"
     gene            3033..3476
                     /locus_tag="VLA60_08880"
     CDS             3033..3476
                     /locus_tag="VLA60_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF09516.1"
                     /translation="MDIFQILKKDHQTVKDLFKKLETTGPRAMKSREKLFSQLKDDLE
                     AHSHGEEAVFYPALRENAEMVDLINEATEEHAEVENLLEDLEAMEVESAEWGSKLAEL
                     KKSVLHHVKEEESEIFKKAKEILDKEEIQKMGKEFQEAKKQATAG"
     gene            complement(3580..4605)
                     /locus_tag="VLA60_08885"
     CDS             complement(3580..4605)
                     /locus_tag="VLA60_08885"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF09517.1"
                     /translation="MKKDQRPEVVVVTGASAGIGRATVRAFARRGGSLGLLARGQEGL
                     EGARREVEKLGGHAVVVPTDVADPEQVERAAVTVEKAFGPIDIWINNAMVSVLSPVKD
                     MTAEEFQRVTNVTYLGYVYGTLSALRRMLPRDRGVIIQVGSALAYRAIPLQSAYCGAK
                     HAIKGFTESLRSELIHDGSRVRITMVHLPGVNTTQFGWIKSRLPYEPQPVPPIYQPEV
                     MARAIVWSADHDRRELWVGMPTAKTIVGEKFIPGLLDYYLAEVAYTGQQTNIPGNPDR
                     ADNLLTPVPQDRGAHGTFDDQARSSSFQLWASMNRGWLAVAGVGCLLSLGWARKRLQV
                     NRSRMND"
     gene            complement(4691..6067)
                     /locus_tag="VLA60_08890"
     CDS             complement(4691..6067)
                     /locus_tag="VLA60_08890"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF09518.1"
                     /translation="MHFRFSSIILIFLIIVVWSARVEPVWSQEPVDTPEVPTDSRPTD
                     KPQEIAVGADKVEDRLITQRLREVFQNLPDLANVDIVVNAGVVRLTGQTSTKEAHKQA
                     LELAERVDGVVSVTDEITQKREVRERLTVVQEKMVDQLNNFISYLPLLIIGFTVFLLF
                     WFLASFMTKWDNLYEKITTHAFLQSLAKQIVKGTVIFIGFVVMLEILDATALLGTIVG
                     VAGVMGLAIGFALRDTVENYIASILLSIRQPFRPLDQIVLENYEGLVMKLTSRETILM
                     TLDGNHVRIPNATVYKGIILNYSRNPNRRFSFEVGIDTAVNIEAALQLAIKTLEQTCG
                     VIADPPVRCTVEKLGDSNVVLKMFAWTTQDQYDFGKVKSEAIRAVKEAFDLANYEMPE
                     PIYRLKMQGYSPLDDHAVFDQTDHTNGAPAPAVPSSSQADVTKDNHIVEQISKDRTEN
                     KERDLLKS"
     gene            6659..7780
                     /locus_tag="VLA60_08895"
     CDS             6659..7780
                     /locus_tag="VLA60_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF09519.1"
                     /translation="MSPQVLSAAFLLLCSLLLYVLALIFAPFMTPILWAMILVRLFYP
                     LYQWLTRKLGDRTTASAALSTLSVMLLAVLPVAYLVFLVVTETINAYQLAMTWVQEGG
                     LERLPELVARLPLIGNVSQELLGRLILAYGDVQGHLVEGGKAVSGVLLSSVGGLATNA
                     IDLVMDFFVILFTLFFLFRDGHHLYRAFYEALPIEESYKAVIMERLDNVMVAVVQGTL
                     LTALAQGFTAGLAYWALGVPFSIFLGALSAILSLVPFGGTALVWAPVAAYLLWTGPIW
                     KGIVMIVIGVGLVGLMDNLLKPFLVGSKADLPVLFLFFASLGGLAYFGFIGLFLGPIL
                     LGIALAVFQIYRENYQEQVGLLVKAETGHPKEPVNPIIK"
     gene            7793..8047
                     /locus_tag="VLA60_08900"
     CDS             7793..8047
                     /locus_tag="VLA60_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HSF09520.1"
                     /translation="MSIIGWIVFGLIVGVVGKLLMPGKDPGGFLATVAIGIMGALFGG
                     MLGRMIGMYGQDDPVGFVMAVIGAILFLWLYRVITRRGDT"
     gene            complement(8200..8856)
                     /locus_tag="VLA60_08905"
     CDS             complement(8200..8856)
                     /locus_tag="VLA60_08905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF09521.1"
                     /translation="MIRVVIFDVDGTLMDTNYLHVEAWARAFSEVKHPVPRAAIHRQI
                     GKGSDLMLGALLTDEALHGRANELHSQFFEELKDHTYPLPGAKEILATLSSQDIAIWL
                     ATSAKSEELEHHVQALEAKDKLAGLVSSADVESAKPAPDIFQLTLERAGCSPKESIVV
                     GDTIWDIQSAQGCGLQTIAVRTGGAFSREELHTAGAVAVYQDCAELLKSGFPTTFEAR
                     "
     gene            complement(9602..10009)
                     /locus_tag="VLA60_08910"
     CDS             complement(9602..10009)
                     /locus_tag="VLA60_08910"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HSF09522.1"
                     /translation="MSFFSKILDKLGFNRSETQAATAPQTSSAPKPSAPQVAPASTAA
                     PTKTTVVDVNGKLEGLAASNPQKLNWKTSIVDLLKLLGLDSSFAARKELAIELGCPAD
                     KMGDSAHMNMWLHKTVLQKLADNGGNIPKELLD"
     gene            complement(10465..11019)
                     /locus_tag="VLA60_08915"
     CDS             complement(10465..11019)
                     /locus_tag="VLA60_08915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HSF09523.1"
                     /translation="MLTEKQKMLKGELYNGADPELVEERQRARLLCQQLNALPATART
                     AERQAILADLFHTETDTDLTPPFFCDYGYNIRLGAHAYFNFNCVLLDVMPISIGSHVL
                     FGPGVHIYTALHPLSAHERRSGLEFAKTVTIGDDVWVGGGAIICPGVVIGDGAIIGAG
                     SVVTRNVRPGVVAAGNPCRVIRQI"
     gene            complement(11197..12000)
                     /locus_tag="VLA60_08920"
     CDS             complement(11197..12000)
                     /locus_tag="VLA60_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF09524.1"
                     /translation="MRPVYLEKKGTMEYHDTIISQFTLQAVPFAELPGHSASMDMLVQ
                     FSGVGAKDTVLDVACGPGIVACELAKHAAHVTGLDLTPTMIQEAGKRQQEQNITNVSW
                     HIGDVLPLPFPSAHFSCVVTRYSFHHFLDPMAVLLEMVRVCKPHGRVMVVDAVLPSEK
                     IDAYNRMEQLRDPSHTKALSFAEMATAIEASGLTNIKTGCYKVEMELERQLAASFPHP
                     EGKDKLRQMFQSDIGKDAMGVGVHRMGSEIHFAYPILITVGQRLPDVVL"
     gene            complement(12241..>12431)
                     /locus_tag="VLA60_08925"
     CDS             complement(12241..>12431)
                     /locus_tag="VLA60_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09525.1"
                     /translation="FASSRVDAFTLGAWLNEGQLAEVVSCARETQEKLRHKALFYRMY
                     VHAESSGRVIGRQLVQKW"
BASE COUNT         3014 a         3288 c         3055 g         3074 t
ORIGIN      
        1 aggacggagg caggacatgg cagccggtca accgcggact gaaatcgcca tatgaacttc
       61 ccgaccccac tgcagaagtc ggccactgtg tgcactgcat cgccagacac ccgtcgcgcc
      121 cgaatgtgct cttcatgcag aagcactggg atgtgatgcg tagcgatgac tcaggcgagt
      181 cgtggcatga aatcagtgga gacttgccct ccgatttcgg attcccgatt gccgtgcatg
      241 cccatgagcc ggagaccatc tatgtggtac cgattaagag cgactccgaa cattatccgc
      301 cggaggggaa actgcgcgtc taccgcagcc ggacaggcgg aaacgaatgg gaagcgctga
      361 caaaaggatt gccgcaacaa gactgctacg tcaatgtctt gcgtgacgcc atggccatcg
      421 actcgctcga accatgcgga atctatgtcg gcaccaccgg cggacaggtg tatgcctcgg
      481 ccgattcggg agatagctgg atgcccatcg tccgcgatct tccacccgta ttatcagtcg
      541 aagtccaggc cttaccgtga gcacgattcg ggttgtcctt ccggctcatt tgcggacact
      601 ggcacgtatc gacggcgagg tgaacctgga gatggagggt cctgtcaccc cacacgcaat
      661 cctcaacgct ctcgaaacac gttacccgat gttgcggggg acaattcgtg accaggtgac
      721 acaacagcga cgaccgttca tcaggttctt tgcctgcggg caggacctct cccatgaacc
      781 accggacgcc ctgttacccg acgcgatcac aactggagaa gaaccgttcc tcatcgtggg
      841 agcgatggcg ggtggctaaa aaccggcaag ggtattcctt ttcccggcca gagaattccg
      901 ttttggaggg gattctccat ccggtttact tggcgtcgtg gaatataatc ccgaccgtat
      961 aacgctgccc tgagcgaacg cggctgaccc catgccggag attcacacga taccagcctc
     1021 ttccccctcg cacagggcga tgatgaaccg cgaaaatgac gccatcgcct tgtcgcatcg
     1081 ggatcacaat cggccgggat tgcatacgtg gccgctgctc ggtcatgaca aattcaccac
     1141 ccgtgaagtc tttctgaggg tcagataaaa gaatggcaag ctggagcgga aacacgtgtt
     1201 cgccgtagag gtcctgatgc aggcagttgt aatcgtcagt ttcatatttg agcattaatg
     1261 gggttggctt atcctgcccg gagtgatgac agcgggccag aaaatcttca tgcctctttg
     1321 gaaagcgaat ctcaatgccc atggccgcat tccaccggtt cgcaaccggc acaagatggg
     1381 ggtacatcgc attccgaagt tgttcgatga gatcaggaag tggatatcgg aaatactgat
     1441 attctccacg gccaaagtta tggcgactca tcacgatgcg gctgcgaaat atctcagcgc
     1501 gggaatacaa catggccaat gcctcgcatt ctttcgaggt gaggagcccc tccgtcactg
     1561 cactttcttg agcgtcgaga tcgcttgaga cctgtcccca atcaatcgcg ttggcccgtt
     1621 ccagcatagt cctaggcaaa gcgactggtg ctgttgattg cccgctccca ttcatgtgtg
     1681 tgcctcacgt tcaagtaacg ctcgtttccg ttcgatgccc caacgatagc cggagagttc
     1741 gccattcgtg cgcaccacac gatgacaagg aatagccaca gctattgcat tggcgccgca
     1801 ggcactggcg accgctcgaa tcgctttcgg cgcaccgatg ctcgcggcga tgtcagcata
     1861 gcttaccgtc gagccggcgg gaatgttttg caatgctttc cagacccgtt gttgaaacgc
     1921 cgtaccttgc acgtccaacg gcaggtccaa cccccgcccc ggcgcttcga caaaaccgat
     1981 cactgttgca accaggcgtt cgaagtcacc atcacccccg atgagatgag cctgggagaa
     2041 ctgcttctgc aaatcgcgcg tcagttgctc agggtcatcc cccagaagaa tcgcacacac
     2101 gccgcgctca cttctggcaa cgaggattga cccaagagaa cattctccca cggcaaatcg
     2161 gatgtctatc ccggaaccac cggcacgata tgaggaaggt gtcataccca aagcagcatc
     2221 ggaagtttca taaaaccttc cgttggaatt gaacccggct tcgtaaatag cctccgtcac
     2281 ggtgccctta tgattcaggt gtgacctcac ccgttttgcc agatgtgccg ccgtgtatcc
     2341 ttttggagtc aatcccgtga ccgctttgaa cacccgatga aagtgatacc ggcttagtcc
     2401 ggcatgatgt gataactctt tgaggcctgg cgcatggtcc gaatcctcga tgaaccggca
     2461 agctgctgcg actttctccg catatcgttc ctgcaatccg ggcaggtttg gcgtgcaccg
     2521 cttgcaggga cgaaagccag ccttctcggc atcttcacgt gaagcatgga accggacatg
     2581 ttcaggccgc ggcaaccgag atgggcagga cggacggcag tacactccgg tggtgttgac
     2641 ggaataaaaa aatgtaccgt cggctgtggg gtcacgggaa accacaattg tccaccgggg
     2701 atcatggcgc gttgtaacgg aacatgtttc ctggtttgct gtgtttctca tgaccactac
     2761 ctgttacagc ctgctaaaat ggcaagcata atttaccagg aaatctcccc tcctgacatt
     2821 cctcttttcc ggatgccaat ctttttatac aaaagtcggg gacagcgtgc actccgtttc
     2881 ttgctttcca attcgcagtt ccctgctact tccagggttc ttaccagttc acgtgtggga
     2941 aggtacaaga ttattcttgg agcaaggaat cgagattttc attatttttt cagtgagtag
     3001 ccatcggtca ttgaccggaa aggaaccagg ggatggacat ttttcaaatt ttaaaaaagg
     3061 atcaccaaac ggtaaaggac ttatttaaaa aattggaaac taccggtcca cgtgcgatga
     3121 aaagccgaga aaaattattt tctcagttaa aagatgatct tgaagctcac tcccatgggg
     3181 aggaagccgt cttctatcct gccctcagag aaaatgcaga gatggtagac ttgattaatg
     3241 aagccacgga agagcatgcc gaagtcgaaa accttctgga agaccttgag gcgatggagg
     3301 tagaatccgc ggaatggggc tcaaaacttg ccgagctcaa aaaaagcgtt ctgcaccatg
     3361 tgaaggagga agaaagcgaa atctttaaaa aggccaagga gatcctggat aaggaagaaa
     3421 tacagaagat gggaaaagag tttcaagagg caaaaaagca agccaccgca ggctaacttt
     3481 taccatgtcc aattgcccag ggatgggcct ccaagtcgag aggctgatct tgcatcaggc
     3541 agatcagctt ctcgatcgga gaatggattt tattgaggtt caatcattca ttctcgatcg
     3601 attcacctgt aaccgctttc gggcccaacc tagcgaaaga agacacccga cgcccgccac
     3661 cgccaaccac ccccggttca tggaagccca tagttgaaaa ctggatgacc gggcctggtc
     3721 atcaaaagtg ccatgtgccc ctctgtcctg cgggacaggc gttaataaat tatcggcgcg
     3781 atcaggattg cccgggatat tcgtctgttg gcccgtatag gctacttcgg cgagataata
     3841 gtccaataac cccggaatga atttttcccc gacgatggtt ttagctgtcg gcattccaac
     3901 ccacaactct cgacgatcgt gatcagccga ccagacgata gcccgcgcca tcacctccgg
     3961 ctgatagatg ggtgggaccg gctgtggctc atagggaagg cggcttttaa tccacccaaa
     4021 ttgagtggta ttgactcccg gcaaatggac catagtaatg cggacccggc ttccatcgtg
     4081 aatcagctcc gatcggagcg attccgtgaa tccttttata gcatgcttgg caccacagta
     4141 ggccgattga agtggaatgg cacggtacgc caacgcacta ccgacttgga taatgacacc
     4201 ccgatcccgg ggaagcatcc gacggagtgc tgaaagggtt ccatagacat atccgagata
     4261 agtaacgtta gtcacacgct ggaattcttc agccgtcatg tctttcaccg gcgacagtac
     4321 ggaaaccatt gcattattta tccaaatatc gatcgggcca aacgccttct ctactgtgac
     4381 tgcggctctt tccacctgct cgggatcggc tacatctgtc ggaacaacga cagcatgccc
     4441 ccctagcttt tccacctccc ggcgggctcc ttccaaaccc tcctgaccac gagccagcaa
     4501 accgagagag cccccccgac gagcaaaagc ccgcacggtg gctcggccga tacctgcaga
     4561 agcccctgtg acaaccacca cctccggtcg ttgatctttt ttcatgtctt ttccttttat
     4621 ggttaacgcg caacgggatt ggggaacggt aacggctggg aatatctcca gccagcttcg
     4681 aaactcaact ctatgatttc aataaatccc tttccttatt ctctgttcga tccttcgaaa
     4741 tttgttcaac aatatgatta tctttagtga catcggcctg agaacttgat ggcaccgcag
     4801 gtgctggagc accatttgta tgatcggtct ggtcaaaaac agcatggtca tcaagagggg
     4861 aatacccttg catcttcaga cgatagatgg gttcgggcat ttcatagttg gcaaggtcaa
     4921 aggcctcttt taccgcccga atcgcttcac tttttacctt tccaaaatcg tattgatcct
     4981 gtgtcgtcca cgcgaacatt ttgaggacca cattcgaatc gcctaatttt tctactgtgc
     5041 atcggaccgg gggatcagca atcaccccgc aggtttgctc taaagttttt atagccagtt
     5101 gcaacgccgc ctcgatattg acagcggtat cgataccgac ctcaaaggag aaacgccggt
     5161 tgggattccg ggaatagttg agaataatgc ctttgtacac cgtagcattc ggtatgcgga
     5221 catgattccc atctagcgtc atgagtatgg tctccctcga tgtcagtttc atgaccaagc
     5281 cttcataatt ttccaggaca atttggtcca ggggccgaaa cggttgcctg atgctcagaa
     5341 gaatgctggc aatgtagttt tccaccgtgt ctcggagggc aaaacctatg gccaggccca
     5401 tgactccagc tacaccaacg atagttccca atagagctgt ggcgtccagg atttccagca
     5461 tgacgacaaa accaataaat atgacggtcc ctttgacgat ttgtttggct aatgactgta
     5521 aaaacgcatg tgtggtaatt ttttcataca ggttatccca tttggtcata aaggatgcga
     5581 ggaaccagaa aagaagaaat accgtgaagc cgataattag aagtggcaga taggagataa
     5641 aattattgag ttggtcaacc attttttcct gcacgacggt caaacgttca cggacttccc
     5701 gtttctgtgt gatttcatct gtcacgctga ccacaccatc cacgcgttcg gcaagttcaa
     5761 gagcctgttt gtgggcttct ttggtggaag tttgaccagt cagacgtaca acccctgcat
     5821 tcaccacgat gtcaacattg gcaagatccg gcaaattctg gaaaacctcc ctaaggcgtt
     5881 gtgtgataag cctatcttca accttgtccg ctccaaccgc gatttcctgt ggtttatcgg
     5941 tgggtcgtga atctgtgggg acctctggag tatccacagg ttcctgggac catacaggtt
     6001 caacccgcgc actccaaacg acaatgatca ggaatatgag gattattgaa gagaatctga
     6061 agtgcatacc attacatcct gtccttattc ccattattgg ctgcttcgag gggccgatca
     6121 acagattgcc ggatctctcc ggaaaaatac gatcaccgtc tcatgcgaga cccgggttta
     6181 ttcaacgact ggagaaagga aacaaatatg aagttcagaa taatctcaga agaattgcca
     6241 atttgcgaaa ggataaataa atcaatgctt gttaagatcc tttttcatag ttcactaatt
     6301 gctatgaatg cttcaacaga caaattcagg tcgtactatc tgaaaacatc cgtcaaaacc
     6361 gcctctgaat acgatggatt ctaggagtag ggatgtggat acttgagagg ttccgcttaa
     6421 tgatttgtct ataataattt aacgggccgg ttcatatgat aaatagaagt gaaatttgct
     6481 tgacttcatt tcaacctgag aagttttcta aaatgcaaat actaggcgaa cggtaagtgt
     6541 tacactgcag aggtaaaaca tgcaggtact cctaattatg atcattgttt cctactcctc
     6601 gaccaagcgg acggcttcag atgtgtcgat acccttaccc tttgcagcgt caacataaat
     6661 gtctccacaa gttctttcag cggccttcct tctgttgtgt tccctcctgc tgtacgtgtt
     6721 ggccctaatt tttgccccct tcatgacacc cattttgtgg gccatgatct tggttcgtct
     6781 cttctatcct ctctatcagt ggctaacccg taagctggga gacaggacaa cagcttcggc
     6841 ggctctcagc acactgagtg tcatgttact ggcggtcttg cccgtcgcgt atctggtctt
     6901 cctcgtggtg acagagacca ttaacgccta tcagcttgcc atgacatggg tgcaggaggg
     6961 tggactggaa agactgcccg aacttgtcgc cagactgcct ctgatcggaa atgtgtctca
     7021 agaattgttg ggccgactta ttctggccta tggggacgtt caggggcacc tggttgaagg
     7081 agggaaagcc gtcagcgggg tcttgctgtc cagtgtcggt gggttggcca caaacgccat
     7141 tgatctcgtg atggatttct ttgtgatcct gtttaccctg ttcttcctgt ttcgcgatgg
     7201 gcatcatctc tatcgtgcat tttacgaagc cctcccgatc gaagaaagct ataaagccgt
     7261 catcatggag cgccttgaca atgtgatggt cgcagtcgtt cagggaaccc ttctcacggc
     7321 tctggcacag ggtttcacgg cagggcttgc ctattgggcc ttgggcgtcc cgttttccat
     7381 atttctgggt gccctgagcg cgattctttc tctggtacct tttggcggca cggcattagt
     7441 ctgggccccg gtcgccgcat atctgttatg gaccggtccc atttggaaag gcatagtcat
     7501 gatcgtcatc ggggtcggat tagtcggtct catggataat ttactgaaac cttttctcgt
     7561 cggatccaaa gcggacctac ccgttctgtt tctcttcttc gcctcattag ggggccttgc
     7621 gtatttcggc ttcatcggat tgtttctggg tcccattctg cttgggatcg cgctggcggt
     7681 cttccaaatt taccgtgaaa attatcagga acaggtcggt cttttggtta aagcagagac
     7741 gggacaccca aaagaaccgg tgaacccaat cattaaatag gagggttgaa gtatgagtat
     7801 catcggttgg atcgtctttg ggttaattgt gggagtagtt ggaaaactct taatgcccgg
     7861 gaaagatcct ggagggtttc tcgccactgt cgctatcggt atcatggggg cgctcttcgg
     7921 tgggatgctc ggccgaatga tcggcatgta tggacaggac gaccccgtgg ggtttgtgat
     7981 ggctgttatc ggggccatcc tgtttctctg gctctatcgg gtgataacca gacgtgggga
     8041 cacctgacgt caacaatttc cgccggatcg tcgagaacgg ttctttcact cactgacgtc
     8101 ccatgcttcc agcccccaca tgagcagaat gaccaaagac gggatctcgt ctggattcct
     8161 ggcgcagata atcacctatc gcagaaagcc cactccttcc tatcttgcct caaatgtagt
     8221 gggaaacccg gatttcagaa gctccgcaca atcctggtat acagccaccg ctccggccgt
     8281 atggagttcc tctcgactaa aggctcctcc ggttctaacc gcaatcgtct gaagcccgca
     8341 accttgagcg gattgaatat cccaaatcgt gtcaccgacc acaatactct ccttcgggga
     8401 acatccagcc cgctccagag ttagttgaaa aatatccggt gcgggcttcg cactctctac
     8461 gtccgccgac gacaccagcc cggccagctt atccttcgcc tcaagtgctt gaacatgatg
     8521 ttccaattct tcagactttg cactggtcgc cagccagatg gcgatgtctt gggatgaaag
     8581 agttgcaagg atttctttgg ctccaggcaa aggataggta tgatctttca actcctcaaa
     8641 aaactggctg tgtaattcat tggcccgtcc gtgtaaggcc tcatccgtaa gaagcgcacc
     8701 cagcatgagg tccgatccct taccaatctg acgatggatt gcagcacgcg gcactggatg
     8761 tttgacttcc gagaaagcac gggcccacgc ctccacatgt aaataattcg tatccataag
     8821 cgtgccatcc acatcgaaaa tcaccactcg tatcatttga aatttctcct cttaatataa
     8881 aatatggcta tccgacactg tgaatttccg tttgggaatt tgaacaaaag gacaaccagt
     8941 cagcgatgct gtaccccttt cccttccaga cctttttttg ttgagacgtt tctcagactt
     9001 taattgctgt tgaagcagcg tcagaacagc cccttcctct gcaggtaaac cacgaagcga
     9061 ttgagaagaa tctcgtttca cctgtttgcg aagtgtttga atgagggtgc ctctttcgtg
     9121 ttgcccagtc gtttagtaac cccttcaatg gctcgataca cattcagttt agcctgcgct
     9181 tgagaattaa acgattggaa ctcttgaaac gctctggccg cctgggcagt ctctgcccag
     9241 gggcgaaaat ctttgacagt agggtcttgc ccggcaattt ccccgcaaga taggaattca
     9301 catcggatga ttcgatggac tgtttttccc cactcgcatc gatatattga aaaagttcat
     9361 accccggaag ctcttgcgat ttcctgacgt ttttcgccaa tctgtggaac agtaaaatcg
     9421 acgccatgcc tgactccact ttttccttta aactgaaatt taagggtaga gccctttacg
     9481 ttgccatggc gtccactcat cgtcgttaat ccgaaggact cgttgtcact ggcatatgtt
     9541 tcattccccc ctcggcagat gaatccattt cctggtttcg tcccggctgt ccctcgcgcc
     9601 gctagtccag aagttccttt ggaatattgc ccccgttatc ggcgagtttt tgcagcacag
     9661 tcttgtgcag ccacatattc atatgcgcgg aatcccccat cttgtcggcg gggcacccaa
     9721 gttcgatcgc aagttccttg cgtgccgcaa agctgctgtc gagacccagg agcttcagca
     9781 gatccacaat cgaagttttc caattaagct tttgggggtt cgaggccgcg aggccttcta
     9841 gcttaccatt gacatccact accgtggttt tagttggagc agccgtcgaa gctggtgcga
     9901 cctgcggggc tgagggtttg ggggcagaag aagtttgtgg ggcagtagcc gcctgtgttt
     9961 ccgaacgatt gaagccgagc ttatcaagaa ttttgctaaa aaaactcatg gcaggtcctc
    10021 cctcttaaag ttaatggcat tgatcggcaa aagaatggct ttattcacat cacatattca
    10081 ggtacctcga atctttcttc gaagtaccga gagtagacaa gactgggaca gaattatttt
    10141 tatcatgaaa cattaggctc ttgtttgaaa tggatacttt tcacttttca tcagaggatt
    10201 cgtctgtccc cacgacatca gacatcacag tcaaactttc ccaggcccta catgattatt
    10261 tttccaagtg tgggggaacg agctcttcac gggaaaaggc aaatccgaac catagcaaca
    10321 tccactttca ggattctggt agttcaggcc tgcctgcgga ttccgtcgcg catagatcgc
    10381 atactcacag gccgcatcat ctctactttc ttacacggcg caacgggggt tagctgaacg
    10441 tgtaatctaa ccggcatcac cgggttaaat ttgacggata acgcgacagg gattgcccgc
    10501 tgcaacgaca cctggtctga cattacgagt gaccacgcta ccagcaccaa tgatggcacc
    10561 atccccgatg actaccccag ggcaaatgat cgccccgcca cccacccata catcatcgcc
    10621 aatggttacc gtcttggcaa attccagacc ggaacgtcgt tcgtgagcac tcagtggatg
    10681 taaggcggtg tagatgtgaa caccagggcc gaacaagaca tgacttccga tcgagatcgg
    10741 catgacatca aggagaacgc agttgaaatt gaagtaggcg tgtgcgccga gacgaatatt
    10801 gtaaccgtag tcacagaaaa atggcggggt gagatccgtg tcggtttctg tgtgaaaaag
    10861 gtccgcaagg atcgcttggc gttcggctgt ccgtgcagtc gcaggcaaag cgttcaactg
    10921 ttgacaaagc aatcgggccc gctgacgctc ttcaacaagc tccggatcgg caccgttata
    10981 taactcaccc ttgagcatct tttgcttttc agtcaacatc gaataccctg atgatttcga
    11041 aataaaagaa gacgaccatc gtattggtca tgtgtaacgt gaacagagcg gcatggaaat
    11101 gtaaaaattg ttttcaaact ttgacaatcg tattgaattt caaaaaacat tccctgaaat
    11161 tcttgactca cgtatcactc cccacctttt cccctctcag agaaccacgt caggcaacct
    11221 ttgtccaacc gtgataagga tcgggtaagc gaaatgaatc tcactaccca tgcgatgcac
    11281 cccaacaccc atggcatctt ttccaatgtc agactgaaac atctggcgaa gtttgtcttt
    11341 gccctcaggg tgagggaagg aagcagccag ctgacgttcc agctccattt cgaccttata
    11401 gcatcccgtt ttgatattag tcaggcccga tgcctcaata gctgtcgcca tctcggcaaa
    11461 actgagagct ttggtatggg aggggtcccg gagttgctcc atccgattgt acgcatcgat
    11521 tttttctgat ggaagaaccg cgtcaaccac catgactctc ccatggggct tgcaaacccg
    11581 aaccatttcc aacaagacgg ccatggggtc aaggaagtga tggaagctat agcgcgtcac
    11641 cacacatgaa aaatgggcac taggaaacgg gagtggcaat acgtcaccaa tgtgccagga
    11701 cacattggtg atattctgtt cctgctgacg tttccccgct tcctgaatca tggtaggagt
    11761 aagatcgaga cctgtcacat gagccgcatg cttagccaat tcgcacgcca caatgccggg
    11821 accacaggca acatcgagaa ccgtatcttt ggcacccacc ccactgaatt gcaccagcat
    11881 atccatcgat gcggaatggc cgggaagctc cgcgaaaggc acggcctgta atgtaaactg
    11941 ggaaatgatc gtgtcgtggt attccatggt gcctttcttc tctaaatata ccggtctcat
    12001 gccaggttca ggggcatgcc tgtactggcc gaccggctgg agtggcggat gagactcaag
    12061 gcttgaacaa acgcaaggcc atttgttctt cgtcgaaata tgcttcaccc atttttaagc
    12121 cgcgttcttc gcgtgcgaat actctaaagc cttctgatgc atagagccgc ttcgctcgca
    12181 agttgagtga aaccaccgtc aagttgatgt gctccagacc ttcgatgcct ctggcacgcc
    12241 tcaccacttc tggacaagtt gtcggccaat tactctgccg gaggactccg cgtgcacata
    12301 cattcgataa aataatgctt tatgtctcaa cttctcctgc gtctctcgtg cacaactcac
    12361 cacttcagcc aattgtcctt catttaacca ggctcccaga gtaaaggcat ctacacggct
    12421 cgacgcaaac g
//
LOCUS       DATIAL010000289         7689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_377389, whole genome shotgun sequence.
ACCESSION   DATIAL010000289 DATIAL010000000
VERSION     DATIAL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377389"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..2131
                     /locus_tag="VLA60_08930"
     CDS             281..2131
                     /locus_tag="VLA60_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSF09526.1"
                     /translation="MVGLAILIGLVIVVILLLPVLLDLNRYRDQYLPVLEQAFHRPVD
                     VQDVRLTLFPKLGFRVRDLTIGDDPAFSPQAFVTIPFAEVEIQWLPLLRRHIQVEHVR
                     LQDPTVHIIRRHDGALNMATVGKDPALHPPEKAGSNPANALKPLFGVFAVERLSMSGG
                     ILQYEDRAQEPFRSYHLEQLDVVTNSVKIGQTASLHVKGVMMPNQLPLEMTGRFGPLQ
                     STFDLPMIDLAGSIGSVEGTANGQVMDGRLELDVQISDLSTDDMPLNIALDKPVFVKD
                     LQAHLMASLIPEGPSKTSTGVRIDPLTVDLQTGGATIHLSGHGTPGRLILSGEAPTLS
                     SVDFPWALSVQRPFSLEQIRFETVIQGTRVDLVFLKTKAFRGNLEAHGRWDGTPAVPL
                     LLFQGTFTNFAVESMMQALRSSSLRLTGVGELHWSLVRAAHLPSGPSTMAGPVRLMIR
                     DGQLIGFDVMQGIEDALQLPDLLEESTGATKFSLIDANVELEGKGLVIRQLTVEAPDF
                     SMTGVGSLPFDESLNLQGNMAVSRTIGDRIIQRFPMAKVAWHQGRLVLPFTVKGTVQK
                     PVLHLDTQSFGQQVKTNVERRIEKVLQGDEQELQQLLQDGADVLKQLFGQ"
     gene            complement(2178..2615)
                     /locus_tag="VLA60_08935"
     CDS             complement(2178..2615)
                     /locus_tag="VLA60_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09527.1"
                     /translation="MSKNILIIDDDDMSRGLLRMVLEYDGCQCVEAENGAKGISLLES
                     KSFDIVILDNAMPVMTGMDFLDRLQHIPRKPDIPIIMVTGFLNAVVRENATRLGAYAI
                     IGKPYDFGELRAIVTQLCPSAGSPKPRSFVVANMSQTYSPPPA"
     gene            complement(3216..5873)
                     /locus_tag="VLA60_08940"
     CDS             complement(3216..5873)
                     /locus_tag="VLA60_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF09528.1"
                     /translation="MIPVSVKLAWREIQSAWSRFLFLFLCIALGVGAIVAVDLFAVNV
                     AQVILGDTRALLGGDVELSWRRAISDKGHKVLDSLTGRDIVLSHVTEIAAMATVNNPT
                     PQAALPKIASQLVELKAIDSAYPLYGRLVAEPDLPTAQLLNPLQSGCSRSPCFGALVQ
                     ESLLIRLNLTVGSELQIGQGNFLITAVLKKEPDKIANGFSLGPRVMISQDALKATALI
                     QTGSRIQERYRLSISNSTSLEPLMGELRGRLSQEGAQVTSFRDAQPRLRRFLDQLNLY
                     LGLIGFTILLVGGIGVACTIQGFLTQKIPIIATLKTLGADSSQIIRLYLTQSLILGGI
                     GSLLGVIVGVVLHRGLPLLLQGIIPEAMPMNPTVAPILRGIVLGILATLAFSLWPLLA
                     IRHVSPALVYRQAVNHSQTITNTQSRLTRWRTILKHWWNDRAQVVVGISMMVGVTGLA
                     MWQAHSLTLGLFFSGACAVAVLLLLGGTGVLYSILRHVPIPQRYLLRHAVRNLQRPGN
                     FTKPMTLAIGIGVMLLTTLTIVQRSLLDLIGNQIPSQAPSFFFIDIQPDQHPQFVDVL
                     QQQFPDSPYNLVPVVRSRLTAINGKTIDPEEHKGQRNGWYFTREYVLTTSRDLPKDNV
                     LTEGQWWDHTKQSDLDGAMSAPSDFPSVSVEKDAAKNLGLTLGSTLTLDIQGVPLVAK
                     VSSLREVDWGSFSINFFMILQPGSFDGAPFTYIATTRVPTTLEVPLQQAIVAVLPNVT
                     AIKVGDVLESIGRIFHQLALGIQALAFLCLVTGAVVMIAAISINRYRRLHELAIVKAL
                     GASRGLLVFSLGVEFGVIGAFAGLVGLGLGCLLSWSLLYFFFDLTWTVDLIILSTGLL
                     LTILLCLATGFLGTYRLLGFPPLSVLRQE"
     gene            complement(5870..6637)
                     /locus_tag="VLA60_08945"
     CDS             complement(5870..6637)
                     /locus_tag="VLA60_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09529.1"
                     /translation="MNVFSSSTSSSPSLTSQTPASRLLISVNHLGMQLRAGTQSVQIL
                     QDISFEVFTNQVVAIVGPSGSGKSTLLGLLAGLDRPTQGSIRIYQTDITTLSETEMAH
                     FRRKHIGYVFQAFHLIPTLTALENVALPLELQGITSGTLRAKTLLQSVGLEHRLHHYP
                     VQLSGGEQQRVALARAFIVRPPLLLADEPTGNLDSSTGAMVIDLLWELHQQHGSTLVL
                     VTHDMSLAARAQRILTLRDGAMVNETAPPLLSSGSEA"
     gene            6796..7458
                     /locus_tag="VLA60_08950"
     CDS             6796..7458
                     /locus_tag="VLA60_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HSF09530.1"
                     /translation="MNRSSDLLDSRAQFPAEPSLSGPLVGKNESLPKIVAFGDSLTAG
                     LGVSSEESYPAQLEKLLQERGFSYEVVNAGVSGETSAGGLRRVEWILKSRPTVVILEL
                     GVNDGLRGLPLEQTYANLRSIIDRLRNAGVMVILAGMRIPPNYGEAYTGEFIEMYKRL
                     AKELSLPLVPFLLEGVAAQPGLNQADGVHPTAEGYTIVAQNAFQTLEPLLKAGPGHQA
                     KE"
BASE COUNT         1922 a         1773 c         2145 g         1849 t
ORIGIN      
        1 attcggactg ttcaaaatct tgatggcatg aaaggccctg aaaatgtagc ctgaggcgtc
       61 gactaacacg acatgacgaa tcggaagagg ggaagacggt tcactcacag tggtcacagg
      121 aaaatatcct ctttgtatga ttgttggccc ttgaagatac ctgatagtcc ttgaggtgtc
      181 gatatcttag gatggtttat ctacggagag aaatggaaca tggtaaacta ccgaatcctc
      241 tggctcatga cgggagcatg tctttgtgaa gagtaatatc atggttggtc tcgctatcct
      301 cattggcctg gttattgtgg tcatcctgct tcttccggtt ttgctggatt tgaatcggta
      361 ccgtgatcag tatttacccg ttctggaaca ggccttccat cgcccggtgg atgtgcagga
      421 tgtccgctta acgctttttc ctaaactggg ttttcgggtg cgggatctga caatagggga
      481 tgaccctgcg ttcagtcctc aggcgtttgt cacgattcct tttgctgagg tggagattca
      541 gtggctccca ctgttgcgcc ggcatattca ggttgaacac gtgcgtcttc aggatccgac
      601 agttcacatc atccgcaggc atgatggggc gttgaatatg gccactgtcg gaaaagatcc
      661 ggctctccat ccgcctgaga aggcgggttc gaatccagcc aatgctctta agcccttatt
      721 tggtgtgttt gcagtggagc ggttatctat gagtggagga atcctacagt atgaggatcg
      781 tgcccaagaa cccttccgtt cctaccacct tgagcaactg gatgtggtca caaattccgt
      841 taaaattggc cagacggcga gtctgcacgt gaagggtgtg atgatgccca atcagctccc
      901 attggaaatg accgggcgat ttggccccct tcaatccact tttgatcttc cgatgatcga
      961 tctcgcgggc agcataggta gtgtagaagg aaccgcaaat ggccaagtga tggatggaag
     1021 acttgagttg gacgtgcaga tttcagacct atcgaccgat gacatgcccc tgaatatcgc
     1081 gttggataaa cctgtgttcg tcaaggacct tcaggctcat ctgatggcat ctctgatccc
     1141 cgaggggccc tcgaaaacat ccacaggagt gaggattgat ccgttgaccg ttgatctcca
     1201 aacgggtggg gcgaccattc atctttcggg tcatggtacg cctggacgtc tgattttgtc
     1261 cggtgaggcc cctactctgt cctcagttga ttttccctgg gcgctttccg tgcaacgccc
     1321 gttttcactg gagcagatcc ggtttgaaac ggtgatccaa gggacaaggg tggacctggt
     1381 gttcctcaag accaaagcgt ttaggggaaa tcttgaggcg catggaagat gggatgggac
     1441 cccagctgtt cccctgcttt tgttccaggg aacctttacg aattttgcgg ttgaatcaat
     1501 gatgcaggcc ttaaggtctt cctccctccg gttgaccggt gtgggagaat tgcattggag
     1561 cctcgtgcga gcggcgcact tgccctccgg gccatcaacg atggccggac cagtccgatt
     1621 gatgatccgg gatgggcaat taattggatt tgatgtcatg caagggattg aagatgccct
     1681 tcagctaccg gaccttctgg aggaatccac cggtgcgacg aaattttcct tgattgatgc
     1741 caacgtggaa ctggaaggca agggactggt gattcggcaa ttgaccgttg aggcaccaga
     1801 tttctcaatg acaggggtgg gaagtctccc ctttgatgaa tcgttgaatc tgcaaggcaa
     1861 tatggccgtt tcacggacga tcggcgaccg gattatccag cggtttccga tggccaaagt
     1921 tgcgtggcat cagggaaggt tggtgctgcc ttttacggta aagggaaccg ttcagaaacc
     1981 ggtactgcat ttggatacgc aatcctttgg gcagcaggtc aaaacgaacg tggagcggag
     2041 gattgagaaa gtcttacagg gggatgaaca ggaattacaa caactcttac aagatggagc
     2101 ggatgttctg aaacagttat ttggacaata gccccggagt acgcttttta tgaaggaatg
     2161 atcccttcat ccttgtctca agccggaggg ggactatagg tttgggacat attggccacc
     2221 acgaaggaac ggggttttgg ggagccggct gaaggacata attgagtgac aatggcccgt
     2281 aattccccaa aatcataggg tttaccgata atcgcatagg ctcctaagcg ggtggcgttt
     2341 tcgcggacga cggcgttgag gaacccggtc accataatga tggggatgtc cggtttccgt
     2401 gggatatgct gtaaccggtc caaaaaatcc atgccggtca tgacgggcat cgcattgtcc
     2461 agaatcacaa tatcaaatga ttttgattca agaagtgata tccctttggc tccgttttcg
     2521 gcttccacgc actgacaccc atcatattcc aggaccatcc ggagtaatcc gcgactcata
     2581 tcatcatcat caataatgag tatgtttttt gacatcaagg tctccttttt taccttatga
     2641 ctgaatccac catcctgatg gatcgagccc cttcaaggtt ggttcaccgt tagtgaaacc
     2701 agtcaaagat ggctcatgaa caactgtcgg gtacgcacag gtctgtgttg ttttggccat
     2761 atctctaccg tgtatgggaa gtgccaggtg cattgggggt tacagagttc tgaccattcc
     2821 aaatgacaag cattcctccg aagattccaa ggcctgtggt acagatggct aaaataattt
     2881 ccatggcttt cctcaaatgt tttgtgtttg ccattttaca aagcaaagaa tgtacccaaa
     2941 acggaagaaa gagatctttt aggggtctca tacatataac tcattgaaat actgatgaat
     3001 ggagagcaaa atttttataa tgggttttga aattctttaa aaaatataaa gaggatgagg
     3061 tattagggca tcagttgtgg gacaggttgt tattcaacac catacagttt gtatctaaat
     3121 ggataacttg taggagaggt ttatctcgga gtgggcccgg catcaggcac agatgtggtt
     3181 ttgggagagg aacaccgcaa ccttttagat gggatttatt cttgacggag tacggacaag
     3241 ggagggaagc ccagcaaccg gtaggttccg agaaagcccg tcgcgaggca tagcagaatt
     3301 gtcaaaagca agcctgtgct taatatgatg agatcgacgg tccaggtcag gtcaaaaaag
     3361 aagtacaaga gagaccagga gaggagacac cccaatccaa gtccaaccag gcctgcaaag
     3421 gctccaatga caccgaattc cactcccagc gaaaatacca gtagcccgcg acttgctccc
     3481 agggctttca cgattgccaa ttcatgaaga cgcctgtacc ggttgattga aatcgcggca
     3541 atcatgacta cggcgccggt gaccagacag agaaaggcca aggcttgaat ccccagggcc
     3601 aattggtgaa agatccgccc aatactctcc agcacatctc ccaccttaat ggctgtcacg
     3661 ttgggcagga cggcgacgat ggcttgctgt aaggggacct cgagagttgt tggcactctg
     3721 gttgtggcga tgtaggtgaa cggggcgccg tcaaaagagc caggctgaag gatcatgaaa
     3781 aaatttatag aaaaacttcc ccaatcaacc tctcgaagac tgcttacttt tgcgacaagt
     3841 ggcactcctt gaatatccaa cgtcagggtt gagcccaggg tgagccccag gttttttgcg
     3901 gcgtctttct ccacggagac cgaggggaaa tctgagggcg cgctcatcgc tccatccagg
     3961 tctgactgct tggtgtgatc ccaccattgc ccctcagtga ggacgttatc ttttggaaga
     4021 tcacgggatg tcgtcaagac gtattccctg gtgaaatacc atccgtttcg ttggccttta
     4081 tgctcttcag gatcgatggt ttttccgttg atggccgtga gacgagacct cacgactgga
     4141 actaaattat acggtgaatc cgggaattgt tgttggagca cgtccacaaa ttggggatgt
     4201 tggtctggtt ggatgtctat gaaaaaaaat gaaggagcct gagagggtat ctggtttcct
     4261 atcagatcaa gtaaggaccg ttgcacaatg gttagcgtgg ttaaaagcat gacaccaatt
     4321 ccaatggcca acgtcatggg tttggtaaag tttccaggcc gttgaagatt cctcaccgca
     4381 tgacgcaaca aataccgctg aggaatcggc acatgccgga gaatgctata cagtactcca
     4441 gtgcctccga ggaggaggag aacggccact gcacatgcac cggaaaaaaa taaaccgaga
     4501 gtcagtgagt gggcttgcca catggccagg ccggtcaccc ccaccatcat gctgatgccg
     4561 accacgacct gtgcccggtc attccaccaa tgcttaagga tggttcgcca tcgggttaat
     4621 ctggattggg tgttggttat agtttgggag tggttcactg cttgtcgata caccaatgcc
     4681 ggagagacat gacggatggc caacagaggc cagagtgaga aggccagtgt ggcgaggatg
     4741 ccgagaacga tcccccgcag gataggagcg acggtgggat tcatgggcat ggcttcagga
     4801 ataatgcctt gaagcaataa gggtaagcca cgatggagca ctacgcccac tatgacccca
     4861 agaagactgc caatccctcc caggataagg ctttgcgtga ggtagaggcg aatgatttgt
     4921 gaagaatccg ctcccagggt tttgagtgtg gcaataatgg gtattttttg ggtaaggaag
     4981 ccttgaatcg tgcaagccac tccgatccca ccgaccaata agatcgtaaa cccgataagc
     5041 ccaagataga gatttaattg gtcaaggaac cggcggagtc gtggctgggc gtcgcgaaat
     5101 gaggtgacct gggccccttc ctggctgagg cgcccgcgta actcacccat caaaggctca
     5161 agagaggtgg agttggatat ggacagacga tatcgttcct gaattcggct tccggtttgg
     5221 atcaacgcgg tggccttgag cgcgtcctgg gaaatcatga cccgtgggcc caaactaaat
     5281 ccattggcaa tcttgtcggg ttcctttttc agtacggcgg taataaggaa gtttccttgg
     5341 ccaatctgca gctcgctgcc aacggtcaga ttgagccgga tcagcaagga ttcctggaca
     5401 agtgctccaa agcacggcga gcgactacaa ccggattgaa gcggatttag cagttgcgct
     5461 gtcgggagat caggttccgc tactaaccga ccatagagag gataggccga atctatggcc
     5521 ttgagttcca ccaattgaga agctattttc gggagggccg cttggggggt agggttgttc
     5581 accgtcgcca ttgcggcgat ttcagtcacg tgtgagagaa cgatgtcacg gccggtgagg
     5641 gagtccagca ctttatggcc cttgtctgaa atcgcccgcc gccatgatag ctcgacatca
     5701 ccgcctaaca gagcacgggt atcccccaga atgacttgtg cgacattcac ggcaaacagg
     5761 tccacggcca caatggcgcc gacaccgagt gcaatgcaga gaaataaaaa tagaaaccga
     5821 gaccaggcgc tctggatttc ccgccaggcg agtttgacag aaacgggaat catgcctctg
     5881 agccggagga taacaacgga ggggccgtct cgttcaccat tgctccatcc cgcaaggtta
     5941 ggatacgttg tgcgcgagcg gccaatgaca tgtcgtgagt gactaagacg agagtgctgc
     6001 cgtgttgttg atgaagttcc cacagcagat caatcaccat tgcaccggtt gagctatcca
     6061 gattgccggt gggttcgtct gcaagcagga ggggagggcg gacaataaag gcgcgtgcca
     6121 aggccactcg ttgctgttcc ccaccagaga gttgaacggg ataatggtgg agacgatgtt
     6181 ctagtccgac ggattgtaaa agtgttttgg ctcgtaaggt gcccgaggtt attccttgca
     6241 attctaaggg gagtgccaca ttttccaggg cagtcagagt gggaatgaga tgaaacgctt
     6301 gaaacacata gccaatgtgc ttgcgtcgaa agtgcgccat ttcggtctcg cttaaggtcg
     6361 tgatgtcggt ctggtaaatg cggatagacc cctgtgttgg tcggtcgagg cctgcaagca
     6421 atcccaggag agttgattta ccgcttccag acggtccgac aatagccacc acctgattag
     6481 taaagacctc gaaggaaatg tcctgtaaaa tctggacgga ctgggtcccc gcccgaagct
     6541 gcatcccaag atgattgacg gaaatgagaa gccgggatgc tggagtttgg gaggtcaaag
     6601 agggcgaaga ggatgtagac gatgaaaaaa cattcacgtt gattacacaa acgggttcaa
     6661 ttggtgaata atagagttaa gaaatcctct ccagctatta tgcatacaag tatcctatca
     6721 gtcctcgtgc tcttctgtct gggaatctgg gggtgtggcc ctgaggagag tccctcacca
     6781 ggtctaccag agtccatgaa tcgcagttcc gacttgttag actctcgggc ccaattcccg
     6841 gccgaaccct ctttatccgg gccattggtg ggaaagaacg aatcccttcc taaaatcgtg
     6901 gcctttggtg atagcttaac ggctggattg ggcgtgtcat cggaggagtc ctatccggct
     6961 caattggaaa aacttttaca ggaacgtgga ttttcctatg aggtggttaa tgccggggtt
     7021 agcggagaaa cctcagccgg aggattgcga cgggtagagt ggattttaaa aagtcggccg
     7081 acggttgtca tattagaatt gggagtcaat gatggactcc ggggcttacc cctggaacag
     7141 acctatgcaa atcttcgtag catcattgac cggttgcgga atgcaggggt gatggtcatt
     7201 ttagcgggca tgcgcattcc gccaaattat ggcgaagcct acacggggga gtttattgaa
     7261 atgtataaac ggctggccaa ggaattgagt cttccactgg ttccattttt attagaagga
     7321 gtggctgctc aacctggctt aaatcaggca gatggagttc atcctacggc agaaggctat
     7381 accattgtgg ctcagaatgc tttccaaaca ttggagcctt tactcaaagc tggaccgggc
     7441 catcaggcca aggaatagat gccacccctc cccgtgaatg ggaaggggta agaaggggaa
     7501 ttctttaaat ggtgtgttat ggaatgcggg aggcgttatg actttttgaa gaaaatgtca
     7561 tagaccccat aggccaagat aactgcgatc attccgtagt acatgcccgt gataccttca
     7621 taccctccgc ctggttcatt ggcaaaggct gggtttgcgg cgaatacgaa aagagcactc
     7681 agaaaggta
//
LOCUS       DATIAL010000290         3200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_806399, whole genome shotgun sequence.
ACCESSION   DATIAL010000290 DATIAL010000000
VERSION     DATIAL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806399"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VLA60_08955"
     CDS             complement(<1..291)
                     /locus_tag="VLA60_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09531.1"
                     /translation="MNLLSEPLTESRKFPRTGLPPASIEPGVPSHSSASKQKRHHYRP
                     VWIVLVLLLPCLALTLYYARYGLSGPLQPDSILPSPSYALVLFLVYLDSVGLV"
     gene            complement(469..870)
                     /locus_tag="VLA60_08960"
     CDS             complement(469..870)
                     /locus_tag="VLA60_08960"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HSF09532.1"
                     /translation="MSEEEPGFVIRDKRGRSEPEASTPPPPKSSMEQPLSSAESSHAP
                     HPPLSFSSFVFSLGTSSLMLMGESLDPQQPAPPMNLPQAKEIVDILSLLEEKTKGNLS
                     SEEASVLGDMLYTLRMKYVSVTSGKGSTVSP"
     gene            998..1918
                     /gene="mazG"
                     /locus_tag="VLA60_08965"
     CDS             998..1918
                     /gene="mazG"
                     /locus_tag="VLA60_08965"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HSF09533.1"
                     /translation="MNQPSRETSTSTSPTTCLSDLLAIMSRLRGPDGCPWDREQTSQS
                     LKPHLLEETYEVLEAIDAGKPSALKEELGDLLLQIMFHTQIASEQNQFSFNDVARGLA
                     DKLIRRHPHVFQQTTDAAPVTTAQDVSRQWDQLKQQEQATQPGPQSLLQGIPKIAPAL
                     QRAFQVQKRASRAGFDWETIEPALEKFKEELNELYAAAAESIPSSSEGTLRPENPTVQ
                     NTIEEELGDVFFALVNVSRFLRVNPEEALRRATNKFITRFHFVESQAALEGKDLRDYT
                     LPQLDKWWDAAKHLERQAPQISESIEGATT"
     gene            1915..2544
                     /locus_tag="VLA60_08970"
     CDS             1915..2544
                     /locus_tag="VLA60_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09534.1"
                     /translation="MSQEEEPTEGRRVGIHPLIVVFGVIFGLWLFITLIIPKSKNLPN
                     QGPGTTPKTPVGLHEIVSTDLIPTYEATGTVTDMKAITILVPPATTDSQVVALLQYFQ
                     NARLDNALDTLLPPTTPGDKLGEFAIADIYIFSEAQYGVPESAKALGRGAHAPGEFYP
                     SSIPYEVAMENVRGHYAIDLYNKNSPERASLGFGEDETGVYSKRYQRVF"
     gene            complement(2617..3024)
                     /locus_tag="VLA60_08975"
     CDS             complement(2617..3024)
                     /locus_tag="VLA60_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09535.1"
                     /translation="MESDLIVLNVMGPYREPREPAFSYDYSVQRPDWATAQGVRVKVS
                     IELELDYLKNSILGIVGGSVGQQLRINQILSRAIADKKLEIADADGLLADRLDVMIGP
                     FTGDSISLFAELDQWMKAEQAVLRKSIHDQTGL"
BASE COUNT          850 a          845 c          788 g          717 t
ORIGIN      
        1 caccaacccc acgctatcca ggtacaccag aaaaagcacc agggcatagc tggggcttgg
       61 aagtattgaa tccggttgca ggggtcctga taagccatac cgggcgtaat agagagtgag
      121 ggccaggcag ggcaacaaca agaccaacac aatccagaca ggcctataat gatgccgttt
      181 ttgcttcgat gcagaactgt gggagggaac acccggctcg atggacgcgg gcggtagccc
      241 cgttcgagga aatttccggg attcagtgag cggttctgag agaaggttca cggcgtgtca
      301 gcgggagaat atcatccaag ggccagtcaa ctatgatcat tattgatcaa gaacaggaac
      361 agcgcaaggc gcgaccacat ccccactatc gattcgtctg tctgcctttg ggcgaattga
      421 gccgcacgcg tctggaagct tgcgggaagg aagagggttc gccgggaatt agggagaaac
      481 cgtactgccc ttccctgacg tgacgctgac atatttcatc cgcagggtat agagcatgtc
      541 tcctaagacg gacgcttctt cagacgaaag attccctttg gtcttctctt ccagcaaaga
      601 taaaatatcc acaatttcct tggcttgggg aagattcatg gggggtgcgg gctgttgagg
      661 gtccagagac tctcccatca gcatgagaga agaggttccg agggaaaaaa caaacgagga
      721 aaatgacaag ggaggatgag gcgcatggga cgattctgcc gaagaaaggg gttgctccat
      781 tgaagacttt ggcggtggtg gagtagacgc ctctggctcc gatcgccctc gtttatcccg
      841 aatcacaaaa cctggttctt cttcgctcat cgaacacctc ctgtagtggg aaaacactag
      901 cactaaccaa tggtctctat caagaatcaa ggacttacac tgagggagaa aacgggtata
      961 atgccctcgc atcgttctca aaaccttttg ccgtctcatg aatcaaccat ctcgggaaac
     1021 ttccacctct acttctccga caacatgttt gtcggattta ctggccatca tgtccagatt
     1081 gcgtgggccc gacggctgcc cttgggaccg tgaacaaacg agccagtccc tcaaacccca
     1141 tttacttgag gaaacgtatg aggtgctgga agccatagat gcagggaagc cttccgccct
     1201 caaggaagaa ttgggggatc tcttgcttca gattatgttt cacacacaaa ttgcctcgga
     1261 acagaatcaa ttttcattca atgacgtggc cagggggtta gccgataaac tgattcgacg
     1321 gcatccccat gtgtttcaac agaccaccga tgctgcacct gtcacaacag cccaggatgt
     1381 gagccggcaa tgggatcaac tcaaacaaca agaacaagcc actcaaccgg gccctcagtc
     1441 acttttacag gggattccca aaatcgctcc agctctccag cgtgcctttc aagtccaaaa
     1501 acgtgcctct cgcgcaggat ttgattggga gaccattgag cccgccttgg aaaaattcaa
     1561 agaagaattg aatgaattat atgctgccgc tgcggaaagt attccgtcgt cttcggaagg
     1621 gactcttcga ccggaaaacc cgacggttca aaacaccatt gaagaagaat tgggggatgt
     1681 gttctttgcg ttagtcaatg tctcccggtt ccttcgagtc aatcctgaag aggcgttacg
     1741 ccgtgccacc aacaaattta tcaccaggtt tcatttcgtt gaatcacaag ctgcgttgga
     1801 agggaaagat cttcgcgact atacgcttcc gcaacttgac aaatggtggg acgcggccaa
     1861 acatctggag cgtcaagccc cacagatttc cgaaagcatc gaaggagcca caacgtgagc
     1921 caggaagaag aaccaacaga gggtcggcgg gtaggcatcc acccccttat cgtggtgttt
     1981 ggcgtcattt ttggtttatg gctgttcatc acgctcatca ttcctaaatc caaaaattta
     2041 cccaatcagg gaccgggaac cacgcccaaa accccagtgg gcctccacga aatcgtatcg
     2101 actgatctca ttccgacata tgaggcgacc ggaaccgtca cagacatgaa agccatcacc
     2161 attctggttc ctccggccac cacagatagt caggtggtcg cgctgttgca atatttccaa
     2221 aatgcccgtc tggataatgc gctagacact cttttgccac ccaccacacc gggagacaaa
     2281 ctcggggaat tcgccattgc cgatatctat attttttcgg aagcccaata cggcgtgccc
     2341 gaatcagcca aggccttagg ccggggggct catgcccctg gggaatttta tcccagctct
     2401 attccgtatg aagtggctat ggaaaatgtc cgcggccact atgcgattga cctctacaat
     2461 aaaaactcgc cagagcgtgc gtccctggga tttggtgagg acgagacagg ggtgtattca
     2521 aaacgctatc aacgcgtctt ctaatacatt tgatgcgaag ggacgaagag tctcatccct
     2581 cctgcagagg ctccctcctc aaaatttctc cggctctcag agtcccgtct gatcgtggat
     2641 agatttccgc aggacagcct gttcagcctt catccactga tccagctcgg caaacagaga
     2701 aatggagtca ccggtaaaag gcccgatcat cacatccagg cgatcagcca gcaatccatc
     2761 cgcgtcggca atctcaagct ttttatctgc aatggctcgc gaaagaattt ggttaatccg
     2821 aagctgttgc ccgactgatc cgcccacgat acccagaatc gagtttttca aatagtccag
     2881 ttccagttca atcgagactt tcactctgac tccctgtgcg gttgcccaat caggtcgctg
     2941 aacggaataa tcgtaggaaa aggccggctc gcgtggctcc cgataaggac ccatcacatt
     3001 caaaacaatt aaatctgact ccataattcc attccttaaa cgaagaatag gtggatctat
     3061 tctacagaag gcgccgacac ccgggattta gacaatattt gaataagggc tccactcgaa
     3121 tccccttcga gggggcccgt ccacccttgt aatccatttt tcagaagata aatattggga
     3181 taattatgtc gactcagaaa
//
LOCUS       DATIAL010000291         3324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_592327, whole genome shotgun sequence.
ACCESSION   DATIAL010000291 DATIAL010000000
VERSION     DATIAL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592327"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..2442
                     /gene="malQ"
                     /locus_tag="VLA60_08980"
     CDS             217..2442
                     /gene="malQ"
                     /locus_tag="VLA60_08980"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSF09536.1"
                     /translation="MNNYESVTKPIWQLGRQYGIEPVYFDGLGVERQVTVPHLQQVLA
                     SMGVKTSSKKEILDSLAHAASRAWTRVIDSVVVRYPSDAPFMLALSLPLGDISLEKVS
                     LVIQVTDEKGGSRRLSLPGSRGKVTSEKTIRGVRYVQVHVPLSGKFSLGYFRLSVQVK
                     VHAERTVEGQALLIVAPRKCYLPPSPKRAWGISLQLYGLRSPRNWGIGDFRDLEEVMK
                     VAGTRWGVATIGINPLHIPAMGLTSPYSPSSRLFWSPVYLNLEGVIEWRTSAGLRRKA
                     KSAKFQRRLRQFRESPLVDYEAVGKLKWTVLEELFRVFRRHHLQPKHLTARGRAFAKF
                     VSEFHPHLTMFCTFQVLAERLGTVAWRTWPDAFRHPQSPDVEVFQRSHATRIRFYQYV
                     QWLCELQLQNLDRVAKKHSLSFRLYHDLPVGIHPDGADSWVFQDQLASDITVGAPPDS
                     FNLNGQSWGLVVPNPVRLREDGYRFLRETLRQNMRHGGVLRIDHALGLFRMFWVPQGG
                     TGKDGVYVKTFVDEILAVLALESVRRKVMVVGEDLGAVTPVIREKLDAAGLLSYRLLF
                     FEREIGGEMTPPHSYPEQALVAATTHDLPTLKGFWTGRDIIIKQEGGVYLRKKDCKQD
                     RQQRAEDRKQLWEALHREGLCSAGTIPPNLSDDILQAMYRYLARTPSRLLIVQLEDLL
                     AELDTPNLPGAPESVYPSWRVRIGRELTAWLNDPAILGFAKAMQRERRKGGSRDQIGR
                     E"
     gene            2650..>3324
                     /locus_tag="VLA60_08985"
     CDS             2650..>3324
                     /locus_tag="VLA60_08985"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09537.1"
                     /translation="MQEKKLTMAGWVDGLTDAVTASLAQIVAYLPTLILACMLLGLGY
                     VLARLVSVVVTRLLQLMGFDRLLSRTAVQTLLERSGTKKKSSEILGLIGFWIIFLVFL
                     IQASDTLSLTMVSDALTSIAYYIPKVGIAVLVLVLGLIAANFVRELITMTCSSAGITH
                     GTMVAQAVYVAVVLLIVVTAIDALGINTELLNNTIVILLAGLIGGAALSFGLGSRTAV
                     ANLIAAH"
BASE COUNT          742 a          749 c          963 g          870 t
ORIGIN      
        1 gagaccagtt taatgggaga gatactctgt tacagggcaa tgattggaag aatgttctga
       61 tctgaggtga cgaacacaaa caatgcggta gatgcatggt tcgtcaacaa gacactctca
      121 tgagccggat gcaacaaacc tgactataag tgatggttaa tagcgatttc cgcaacaact
      181 gccatcaaga agagtatctt tttttgaagg acaccgatga ataattatga atctgtcacg
      241 aagccgatct ggcaattagg tcggcagtat ggcatcgaac cggtttactt tgatggttta
      301 ggtgttgagc gacaggttac ggtgcctcat cttcaacaag tcctggcaag catgggggtg
      361 aagacctcat cgaagaagga aatacttgac tcgcttgcgc atgcggcctc ccgagcctgg
      421 acacgggtca ttgattcagt tgtggtgcgc tatccttctg atgccccgtt catgttggct
      481 ctgtcccttc ctcttgggga catcagtttg gagaaggttt cccttgtcat ccaggtgacg
      541 gatgagaagg ggggcagccg gcgcctatct ctgcccgggt ccaggggcaa ggtgacgtct
      601 gagaagacga tccggggtgt gaggtatgtc caggttcatg ttcctctgtc gggaaaattt
      661 tcactcggat atttccgtct ttcggtccaa gtgaaggtgc atgcagagcg aacagtggag
      721 ggacaggccc tgctcatagt ggcaccaagg aaatgttatc ttcccccttc acccaaacgg
      781 gcttggggga tttcgctcca actctatggg ttacggtcac ccagaaactg gggaattggg
      841 gattttcgag atttggaaga ggtcatgaag gtggcgggga ctcgttgggg agtggccacc
      901 atcgggatca atcctcttca tatcccggcg atgggattaa cgagccccta ttctccgtcc
      961 agccgtcttt tctggagccc cgtgtacttg aacctggagg gggtaattga gtggcggact
     1021 tcggcaggtc ttcgtcggaa ggccaagagc gcaaaattcc agcgtcgtct ccgacagttt
     1081 cgggagagtc cactggtcga ttatgaggcc gtagggaaac tgaaatggac agttctcgaa
     1141 gaactattcc gagtgtttcg acgacaccat ctccagccta agcacctcac ggcacgtgga
     1201 agagcatttg ccaaatttgt gtccgaattc cacccccatc tgaccatgtt ttgtacattt
     1261 caggtcttgg cggaacgcct gggaacggta gcttggcgta cctggccgga cgcctttcgg
     1321 catccccagt cacctgatgt ggaagtgttt caacgatccc atgcaactcg gatccgattt
     1381 tatcaatatg ttcaatggtt gtgtgagttg caattacaga accttgatcg tgttgcgaag
     1441 aaacattcgt tgtcgttccg gctgtatcac gatctaccgg ttggtattca tcccgatgga
     1501 gccgattcct gggtctttca agatcagctg gcctcagaca ttaccgttgg cgcgccgccg
     1561 gactccttta atctcaatgg acaaagttgg ggactggttg ttcccaatcc cgttcgattg
     1621 cgggaagatg gctatcgctt tcttcgagaa accttacgcc aaaatatgcg acatggaggt
     1681 gtcctcagga ttgaccatgc cttggggctg tttcggatgt tctgggttcc ccaaggggga
     1741 acaggaaagg atggcgtgta tgtcaaaacc ttcgtggatg aaattttggc ggtcctggct
     1801 ctagaaagtg tgcgacgaaa agtgatggtg gttggggaag accttggtgc ggtcactcct
     1861 gtcattcgag aaaaattaga tgccgccggg cttttatcct atcgtctgtt attttttgaa
     1921 cgagaaattg ggggagagat gaccccgcca cattcctatc ctgagcaggc gcttgtggcc
     1981 gcaacgacac atgatcttcc taccctcaaa ggtttttgga ctggacgaga tatcatcatt
     2041 aagcaagaag ggggtgtcta tctcagaaaa aaagattgca agcaagaccg gcagcagcgg
     2101 gcggaagatc ggaagcaatt atgggaggcc ttacatcggg agggcctctg ttctgccggg
     2161 acaattcccc ctaacctttc ggacgatata cttcaagcca tgtaccggta tttagccagg
     2221 acaccctcgc gcctgcttat cgtgcagctt gaagatttgt tggcggagct tgatacgcct
     2281 aatctgcctg gagcaccgga atcagtctat ccatcctggc gagtgcggat tggtcgagaa
     2341 ttgactgctt ggctcaacga tccggccatc ctgggctttg ccaaagcgat gcagcgtgag
     2401 cgacggaaag gcggaagtag ggaccagata ggcagggaat aggtttcacg cgcgctttga
     2461 tagatggatg gacagccagg cggtatccca tgagtcaaat acggtgtttt gccggaatgt
     2521 cagttgcgtg ctgtgtagga ggatactatg atcaacgatt ggcatggtcc actgcccatc
     2581 gggtctgatg gggaaagcag ggcgtttcgt ctaagttagg tcgatacgga agagaggacg
     2641 atccttcata tgcaagagaa aaagttgacg atggcggggt gggtagatgg cttaaccgac
     2701 gcggttacag cgagcctggc tcaaattgtt gcctatttgc caaccctgat tttggcgtgc
     2761 atgctgttgg gtcttggtta tgtgctggcc aggctggttt cggtcgtggt gacgcgcttg
     2821 ttgcaattga tggggtttga ccgtttgctg agtcgaaccg ccgtgcaaac cttattggag
     2881 cgatcaggaa ccaagaagaa aagttctgaa attttggggt tgattggttt ttggattatt
     2941 ttcctggttt ttctcattca agcgtccgac accctcagtt tgacgatggt ctcggatgcc
     3001 ttgacgagta ttgcctatta tattcccaaa gtcgggattg cggtgttggt gctggttctg
     3061 ggtttaattg ccgcgaattt tgttcgtgag ttgattacca tgacgtgtag ttcggcagga
     3121 attacccacg ggacgatggt ggcccaagcc gtctatgtgg cggtcgtgtt gttgattgtg
     3181 gtgacggcca ttgacgcact cgggattaat acggaattgc tgaacaatac cattgtcatc
     3241 ctcttagcag gattgatcgg gggtgcggca ttgtcgtttg ggttggggtc acgaaccgct
     3301 gtagctaatc tcattgccgc tcat
//
LOCUS       DATIAL010000292        24596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_270698, whole genome shotgun sequence.
ACCESSION   DATIAL010000292 DATIAL010000000
VERSION     DATIAL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 24596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24596
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270698"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="cmk"
                     /locus_tag="VLA60_08990"
     CDS             complement(<1..433)
                     /gene="cmk"
                     /locus_tag="VLA60_08990"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HSF09538.1"
                     /translation="MATRLGYVYLDTGALYRAIAWKVQQEQLDPNNLAHIQGLLARTN
                     LRLMLSKDALSILVDGQAINQGVIRTPDISQLASCIAAIQSVRDWLLPIQRKVVGEVG
                     IIAEGRDMGTRVFPSADVKFFLDAELEVRANRRQQELGQQGK"
     gene            complement(543..1850)
                     /gene="aroA"
                     /locus_tag="VLA60_08995"
     CDS             complement(543..1850)
                     /gene="aroA"
                     /locus_tag="VLA60_08995"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HSF09539.1"
                     /translation="MSQFTIIPTKKPLRGSLSVPGDKSITHRALIFGALAQGQTRIVG
                     YSKGEDCLNTLNAIRALGAGVQETPEGLEVTGKGLWGLQEPSNVLDCGNSGTGLRLLA
                     GVLAGQHFFSVLTGDSSLRSRPMGRVVTPLRQMGAVILGRRAGELAPLAIQGTGLKGI
                     LYESPVASAQIKSCVLLAGLFAEGTTIVKEPRRSRDHTERLLSYLGVPLQVEGSTVQL
                     QGRPSFDGKPIEVPGDISAAAFFMVAASIVPDSDILLTDIGLNPERTGILDILLEMGA
                     DITIVNQREISGEPVGDIRVRSAPLRGMTIGPELIPKTIDELPILCVAAALAHGQTRI
                     TGAQELRVKETDRIRAMATELSRLHVEVEEQPDGLIINGGSQIKGAVCQSYGDHRVVM
                     SLAICGLVAESPITIEDVACVETSFPGFKDKMLDLLTNSERPL"
     gene            complement(1847..2734)
                     /locus_tag="VLA60_09000"
     CDS             complement(1847..2734)
                     /locus_tag="VLA60_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HSF09540.1"
                     /translation="MSTSHSEPLFRHVSIIGIGLLGGSLGLALKEQGLAKSVVGIGRR
                     QENLELAVRMGAIDQFALEPHWAVSQSDLIVLATPVETYLSQIDLWGKDLAPSAIVSD
                     VGSVKGQLVSKIEARLPPSTFFIGAHPIAGKEKSGVAHADSHLFQGARCILTPTPHTN
                     AQALERVRHLWETVGSVVALMDPMDHDWVFGAVSHLPHIAAFSLMHTLEKLQGRTSQP
                     ADLLNFSGGGLRDTTRIAASSPEMWRDICVANHGNLVEMVDRYIQQLQNFRELLSKRD
                     APGLYEAIARAKASRERLI"
     gene            complement(2772..3785)
                     /gene="aroF"
                     /locus_tag="VLA60_09005"
     CDS             complement(2772..3785)
                     /gene="aroF"
                     /locus_tag="VLA60_09005"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSF09541.1"
                     /translation="MVIVLKPEATEQNVDHLIDRLRSLGMVTQITKGKERTIVAVLGD
                     DRVLQGQPLSVFPGVESVTPILAPWKLVSREFQKHDSVIDVAGVKVGGSRIVIMAGPC
                     AVEKLEITVGIAQEVARAGATILRGGAYKPRTSPYSFQGLGQEGLEFLAEARRQTGLP
                     VVSEILDARDLGHFLEKADVIQVGARNMQNFELLKEVGAYDKPVLLKRGLSATIKELL
                     LSAEYIMSRGNRNVMLCERGIRTFEPQYRNTLDLSAVPTLKEMTHLPVIVDPSHATGN
                     WKLVAPMAKAAIAAGADGLLIEVHSNPECALCDGEESLKPSRFAELMNDLQKVAQAVG
                     REL"
     gene            complement(3808..4893)
                     /gene="hisC"
                     /locus_tag="VLA60_09010"
     CDS             complement(3808..4893)
                     /gene="hisC"
                     /locus_tag="VLA60_09010"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSF09542.1"
                     /translation="MPLRVHPTIASLTPYSPGKPLDELERELGITQAIKLASNENPWG
                     PSPNALQVLAGAAASLHRYPDGGAHYLRQALAERWKVSDDQVLVGNGSDEIISLLVRA
                     FLSPGDEAVMADLTFVMYKLSVLGGHGVPVEVPLKNWVHDIAAMVDAVTDRTRLFFLC
                     NPNNPTGTMLTAREIDGFLSRLPDHVVVVFDEAYYEYVRHPDFPDSLQYVRDGRPVVV
                     LRTFSKIYGLAGLRVGYGYTTKEIAGYVNRVRPPFNVNSLAQEAARAALSDDDHVAKS
                     RAMNEAEMTFLEERLITMGLEPVPSQANFLYFNVGIDGRRAYDALLREGVIVRHIRGP
                     MLRVTIGQPTENRRFLESLARVLPTLS"
     gene            complement(5023..6105)
                     /gene="pheA"
                     /locus_tag="VLA60_09015"
     CDS             complement(5023..6105)
                     /gene="pheA"
                     /locus_tag="VLA60_09015"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HSF09543.1"
                     /translation="MELPEDMQQCRQEIDRLDDDILNILNERSQYVIKIGNLKKQQDS
                     SAHLHTPGREVAIVERLMRKNPGPFPSEALRHVYREIMSASLSLEGTQTVAYLGPPAT
                     FTHLAAMRKFGGSADYVSVNSIKDVFDEVERGRMRFGVVPIENSTEGVVNHTLDLFVD
                     SPLLIYGEVMLEVSHHFLSKAEKLEEIKTVYSHPHALAQCRNWLETNLPSVNFAEEPS
                     TARAAERCVNDPNAGAIASELAAQLYGLNILRSRIEDNINNFTRFLILSQKGAEPTGR
                     DKTSIIVSAKDRVGALYDLIRPFSSHGISMTKIESRPTRRKVWEYLFYMDLEGHQDDD
                     RLKKALEEVRSRCLMMKVLGSYSSHQ"
     gene            complement(6261..7220)
                     /locus_tag="VLA60_09020"
     CDS             complement(6261..7220)
                     /locus_tag="VLA60_09020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF09544.1"
                     /translation="MKKRFKQYALKLVKGLPARFSLSVSWNSTEGYMLGYAKEIMRAL
                     ENDYGVTTRRDVITGLRRLSLDEFGLVLLSMPDPEYPKLSRLLPAMASDEVQRGWTGN
                     HGLALLKETTAFVRALSYNYARVTGRSLDDATVLDFGCGYGRIAQLLYYFTVEDKIFG
                     VDPREESIELCRESRLGTNFLVSDYLPVSLPVGGVKFDLIYAFSVFTHLSRRAMTTSL
                     HTLRQHIADDGLLAMTIRPVEYWEQAPHASQDQKDALAVRHRQEGFAFNPHNWAPIDG
                     DVTYGDSSMTLDWMEATFPDWTIKETDRCLNDPLQRYVFLVPR"
     gene            complement(7822..8652)
                     /gene="panB"
                     /locus_tag="VLA60_09025"
     CDS             complement(7822..8652)
                     /gene="panB"
                     /locus_tag="VLA60_09025"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HSF09545.1"
                     /translation="MSAHNPQVIQKLAHMTIPEFTRQKKQGKKLTVVTAYDALFTRIV
                     EQAGLDVILVGDSLGMVVQGKPNTLSVTMEDMLYHTRLVAQAAKRAMVIGDMPFMSYQ
                     ASVEDAVRNAGRFLQAGAAAVKLEGGISVIDRVEAMTRFGIPVMGHVGMTPQSVNQAG
                     GYSVKGKAQDEAETIVSDAKALEAAGAFAVVLECMPTELAQRITEALSIPTIGIGAGQ
                     GCDGQVLVLYDLLGLFDDFVPKFVKPYAHLKADALQALRRYKEEVECGKFPTDAESYH
                     "
     gene            complement(8639..9616)
                     /gene="folD"
                     /locus_tag="VLA60_09030"
     CDS             complement(8639..9616)
                     /gene="folD"
                     /locus_tag="VLA60_09030"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HSF09546.1"
                     /translation="MTSKAAANWTGGAYEEVREPGQGARTPMSAFFNTPGEGVDVPAQ
                     IIDGKALAQAIRESIAEEVRLLEKDTGVKPGLAAVLVGDDPASAVYVRNKKIACEKAG
                     LYPQEHRLPASTTQETLLQLIHQLNADTRIHGILVQLPLPPVMDSQAILQAVSPEKDV
                     DGFHPVNVGRLVTGDPVFIPCTPKGVIQMIESTGQDISGKRAVVIGRSNIVGKPVAML
                     LLHRHATVTICHSRTQDLPAVVREADIVIAAIGKPLFVTAEMVKPGAIVIDVGINRLA
                     DGKLVGDVDYDLVKERAGWLTPVPGGVGPMTIAMLLQNTVESAKRNVGT"
     gene            9899..10873
                     /locus_tag="VLA60_09035"
     CDS             9899..10873
                     /locus_tag="VLA60_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09547.1"
                     /translation="MKPYCLTASFRNAILTSLLLLSCWTAIYAEEVTNLAFPQSMVAD
                     EGQVYFIANANGDPGTRENKGFISKVTTEGKMIDLHFIQGGQKSVTLNSPKGMALVGS
                     TLYVADLDVVRGFDKNTGQSVNSIPFTQFHSQSLAGLVADPHGGLYVSDADSNTIYHI
                     DDPAHDHTVKVFAQHESLSHPRGLSIHPRNGHVVGVGWEDGKIFEIDETGTIHELFAN
                     TLFTGSFHNLDGIDFDKYGTMYVSDLTAGKVWRILANHKKEVIAEFLLSPSGIGIDRV
                     NHVIMVPYLYVNGAEINGLERPSNANPNKKPRSWSDYGMGWLKKDPGE"
     gene            10870..11541
                     /locus_tag="VLA60_09040"
     CDS             10870..11541
                     /locus_tag="VLA60_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09548.1"
                     /translation="MSKCLYCHQRKGKRACPALGGVICSQCCGTNRITNISCPTSCVF
                     LESNDDYQQKRVGNRFELERRGFYRQLLDLGGERATEIFYVFEALAYRFFQDRRDAED
                     AEVLEGLQSLRRSFSLIQIPEAGLPAFGEELRKEFKVFGERQPLEPTLVTNVLDQAHT
                     FIQRFSGPGLRSHRFLHGLLGYIRERHPDVAEQLARQTGAGGRIIIPSGFGYAPDHAS
                     TEPVN"
     gene            complement(11580..12206)
                     /locus_tag="VLA60_09045"
     CDS             complement(11580..12206)
                     /locus_tag="VLA60_09045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HSF09549.1"
                     /translation="MRGVLQPRTLASGLRQLMMVIGLFTIVTAGGSPVANEAAAEEPS
                     QAETKAPHVLIKTKFGEMEAVLFPDLAPKHVESFLKLAKSGFYNGTIFHRIIPGFMIQ
                     GGDPLTKDPANRSRYGTGGPGYTVPAEFNRIIHEKGILSAARTADPNSAGSQFFIMAD
                     KAPHLDGQYTVFGEVVKGLDVIDTIVSQPRDLKDNPLERIEMTIDLIQ"
     gene            complement(12223..12972)
                     /gene="rnc"
                     /locus_tag="VLA60_09050"
     CDS             complement(12223..12972)
                     /gene="rnc"
                     /locus_tag="VLA60_09050"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HSF09550.1"
                     /translation="MAALAFEDIQESLEYEFKQQALLCEALTHRSFSQTQPQAVSPHN
                     ERLEFLGDAVLGLVVSESLAAMFPHSTEGELSKIKAGLISRSTLSKAASRLQLGQWLR
                     LGRGEEATKGREKISLLANALEAIIGAVYLDGGLDASRAFIQKVLTAEFSSLHNSPVS
                     SVGWDGKSRLQEWTHKQFGASPQYRLVRESGPDHQKVFAVTVEIQGKIMGQGEGRTKK
                     EAEQAAAAQAMAQAGLEVLDEPDHSIKKSTD"
     gene            complement(13144..14397)
                     /gene="fabF"
                     /locus_tag="VLA60_09055"
     CDS             complement(13144..14397)
                     /gene="fabF"
                     /locus_tag="VLA60_09055"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HSF09551.1"
                     /translation="MTEWRPRRVVVTGLGLVTPLDIGVSNTWDKLCKGQSGIGPITRF
                     DASQYSVQIAGEVKNFDPSTFIEKKEIKKMDTFIHFAIAASQEAVDDAKLVVNPDEAD
                     RVGVYIGAGIGGLPAIEHYHKVLLERGPDRVSPFFIPMVIINLASGQVAIRFGAKGPN
                     ACTVTACATGNHCIGDGFRLIQRGEADVMLVGGAESTICPTAVAGFAAAKALSRRNDE
                     PERASRPFDKDRDGFVIGEGAGVLILEELEHAKSRGAHIYAEVIGYAMNSDAFHITAP
                     PDDGAGAVTCMERAIQDAGIAKESVGYINAHATSTFADRIETQVIKHVFGDRAYSIPV
                     GSTKSMTGHLLGAAGGIEAVFSIMAMHHGIIPPTINLETPDPECDLDYIPTHARPCDI
                     DVAISNAFGFGGVNACLVFRRWKGE"
     gene            complement(14519..14749)
                     /gene="acpP"
                     /locus_tag="VLA60_09060"
     CDS             complement(14519..14749)
                     /gene="acpP"
                     /locus_tag="VLA60_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HSF09552.1"
                     /translation="MAVEERVKKIIVEQLGVEEDDVVPEAKFVEDLGADSLDTVELVM
                     ALEEEFEIEIPDEDAEKIQTVSAAIDFIKEKV"
     gene            complement(14806..15552)
                     /gene="fabG"
                     /locus_tag="VLA60_09065"
     CDS             complement(14806..15552)
                     /gene="fabG"
                     /locus_tag="VLA60_09065"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HSF09553.1"
                     /translation="MLLTEKIAVVTGGAQGIGQAIATTLAKEGADVVVADLDAERCQE
                     TVDLVQQMGRKAIAVSVNVGEWEQAKGMMERVLKEWGRVDILVNNAGITRDGLLMRMK
                     EEDWLAVLQVNLTGTFFCAKAVLPSMSKQRSGRIVNIASIVGAIGNMGQANYAASKAG
                     VIGLTKTIAREYASRNITVNAVAPGFIDTAMTQHLSPEIKEGLLNQIALKRLGQPSDV
                     ADAVCFLSSEKAGYITGHVLHVNGGMHMAS"
     gene            complement(15591..16526)
                     /gene="fabD"
                     /locus_tag="VLA60_09070"
     CDS             complement(15591..16526)
                     /gene="fabD"
                     /locus_tag="VLA60_09070"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HSF09554.1"
                     /translation="MNPVFGFLFPGQGSQSVGMGKTLFDRSPAVQALYKEAADILGYD
                     IAALCFEGPSERLNQTEYTQPALLVTSLAAFQLVRNGPLLPAAVAGHSLGEYAALVAA
                     GALNFGEAVNLVQKRARYMAEAVTPGSGLVAAILGLSEKDVRSVCQEAGSMGIVAPAN
                     FNSPGQTVIAGEKAAVEHAAELAKTRGAKRVMPLPVSVPVHTPLMQVAADRLKKDIDS
                     LKWSALKVPLVNNVEAKALLSAEEVRESLVRQLPSPVRWQETIQAMALLGITHFVEIG
                     PGKVLTGLVKRIVPEATTWNVMDEESYAHVLSHCA"
     gene            complement(16601..17416)
                     /locus_tag="VLA60_09075"
     CDS             complement(16601..17416)
                     /locus_tag="VLA60_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF09555.1"
                     /translation="MSGTTSWGENDWALILGVSSGFGEAAAMELANLGLNICGVHLDR
                     KSTQANAERIASQIRSLGREVLFFNVNAADPEKRGDILKALKQRAEESGKPVCVRVLL
                     HSLAFGTLKPYIAESHAEAINKAQMDMTLDVMANSLVYWTQDLVAQKFLGTGSRIFSM
                     TSAGGARVWRTYGAVSAAKSALESHTRQLALELAPLGITVNSLMAGVTDTPALRKIPG
                     SDEMLTLAKRKNPSNRLTTTQDVADALGLLSHPKAHWITGNVICVDGGEFIVD"
     gene            complement(17538..18512)
                     /locus_tag="VLA60_09080"
     CDS             complement(17538..18512)
                     /locus_tag="VLA60_09080"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HSF09556.1"
                     /translation="MNSRILGTGAYVPKRVLTNADLERMVETSDTWIIERTGIRERRV
                     VDPGQACSDLAVEAAQRALIAADVAPSEIDLILLATCTGDSPLPSTACLIQHRLGANR
                     AAACDISAACCGFIYALAIADAYVKNGMRHVLVIGSEVMSAITDWTDRNTCVLFGDGA
                     GAVVLGQGTEGSGILSTHLHANGGLSDMIQVPGGGSREPASKEVLQEKRCFIKMKGNE
                     TFKIAVKSMEEATKEALEANKLAMDDIDLFIPHQANMRILNAVGQRLGLAPEKLMINL
                     DRFGNTSAASIPLALDQAVREGRIHKGSVVLLAAFGAGLTWASAVVRW"
     gene            complement(18534..19529)
                     /gene="plsX"
                     /locus_tag="VLA60_09085"
     CDS             complement(18534..19529)
                     /gene="plsX"
                     /locus_tag="VLA60_09085"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HSF09557.1"
                     /translation="MKIAVDAMGGDHGLSPNVEGAIQATRESSLSIILVGDEPSLQSH
                     LDKLGGTKAGIEIFHAPQVVDMHESPALIARKKRDSSIWKATELVKNKQADALVSAGN
                     TGATMVSAFFLLGLIQGVERPAIAATLPTRQGKAIMLDVGATVDCTARQLFQFGIMGH
                     EYGRHLLGTDRPRVGLLSIGEEDTKGNEVTKETFKLLKDSPINFIGNVEGRDVYSGNA
                     DVIVTDGFIGNVALKISEGLADAIKKMLMKEIAQSALGRLSYLFVAGPLLRLRRKTDY
                     AEFGGAPLLGVEGISMICHGRSSSKAIKNAILRAQALAEGGLIEEIRGDIAQGCR"
     gene            complement(19575..19757)
                     /gene="rpmF"
                     /locus_tag="VLA60_09090"
     CDS             complement(19575..19757)
                     /gene="rpmF"
                     /locus_tag="VLA60_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HSF09558.1"
                     /translation="MANPKHKISKARRDKRRSHLKLTPPNLSTCPQCHEPKPSHTTCL
                     NCGTYKGIAIISVEEG"
     gene            complement(20467..20694)
                     /gene="rpsR"
                     /locus_tag="VLA60_09095"
     CDS             complement(20467..20694)
                     /gene="rpsR"
                     /locus_tag="VLA60_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HSF09559.1"
                     /translation="MEKGRLFQRKRVCRFCFDKTPLDYKDANLLRNFLTERGRIIPRR
                     TSGNCLRHQRKLTQAIKRARHVAILSFAEEH"
     gene            complement(20718..21128)
                     /locus_tag="VLA60_09100"
     CDS             complement(20718..21128)
                     /locus_tag="VLA60_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HSF09560.1"
                     /translation="MVGFNKVILIGNLTRDPELRYTPSGTPVVNFPLAVNRRYRQADD
                     QKEEVCYVDIVVFGRQAEHCGQYLNKGDGAIVDGRLQQRRWETDDGQKRSKHEVVAQT
                     VTFLPKKSSSGTSGAGVSEESTYDPEEYYPDPMP"
     gene            complement(21112..21459)
                     /gene="rpsF"
                     /locus_tag="VLA60_09105"
     CDS             complement(21112..21459)
                     /gene="rpsF"
                     /locus_tag="VLA60_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HSF09561.1"
                     /translation="MKLYESLCILRPVQAETENERVIEKMKEVLNQAGANILKLDNSG
                     KKKLAYDIQHERKGTYITVQFEGPPTVVFELERFQRMEDQVMKFMTVRLNPSDLVAVG
                     EASVEDAEDGGIQ"
     gene            complement(21960..22532)
                     /gene="pth"
                     /locus_tag="VLA60_09110"
     CDS             complement(21960..22532)
                     /gene="pth"
                     /locus_tag="VLA60_09110"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HSF09562.1"
                     /translation="MVVGLGNPGESYRYTRHNLGWMVLEQAADRWRVTWAKQEDGFLG
                     QGEVKGYPVLLLLPLTWMNQAGVAVRSVLQKLSPNSPDLIVVHDDLDLSLGVLKIKTR
                     GGAGGHNGLRSILSCLETEEFSRVKVGIGRPQNNESLANFVLSPFSSEEWEQVVSILP
                     KAVDALECLICEGPGIAMNRFHVRSSETGL"
     gene            complement(22542..23261)
                     /locus_tag="VLA60_09115"
     CDS             complement(22542..23261)
                     /locus_tag="VLA60_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HSF09563.1"
                     /translation="MKYELEVEKRDQAGKGVARQLRRQGKIPGVLYGGGKSEFVAMDH
                     KTARNLVISQVGHTGLLTVRISGGQERIAVLQDHQIDPITGAILHVDLFEVSMKKAIR
                     VKVPVTITGEVPVGVKEGGILHQVMRELHIECLPAQIPDHIEIDASGFGIGDGMHVKE
                     VTVPTGLKILDDEDLMVAHVATKMSEAKLESLLAREVAEGVAPVATAEKAAEVAATGG
                     AVAAADKTKAASDTKAKEGKK"
     gene            complement(23307..24248)
                     /locus_tag="VLA60_09120"
     CDS             complement(23307..24248)
                     /locus_tag="VLA60_09120"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HSF09564.1"
                     /translation="MLRDLKLITGNSNLALAREIAQYIGQDLSQATVTTFSDGEIRVR
                     IDENVRGGDVFLIQSCCHPVNTSIMELLLLIDAVKRSSASRITAVIPYYGYGRQDRKD
                     QPRVPISAKLIADLITAAGANRILTMDLHAGPIQGFFNIPVDHLYAMPVLLDYIKKQN
                     VSDLVIVSPDAGGVERARAFAKRLNSSLAIIDKRRESPNQAEVMNIIGDVQDKHALLF
                     DDMIDTAGTIVQTAQTCMDNGALSAWAGSTHAVLSGPALERLQSSCLREVMVTNTIPL
                     NGKDQQCPKLKTLTVAPLLGEAILRIHREESVTSLFV"
     gene            complement(24361..>24596)
                     /locus_tag="VLA60_09125"
     CDS             complement(24361..>24596)
                     /locus_tag="VLA60_09125"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09565.1"
                     /translation="ILGFIKDTLLSFGAQAALLSGSGATVFGIFRTQEEARQASTRLR
                     HDTSWKIFDIPMGSTDLPHDPFHVGSSSQVSKV"
BASE COUNT         6240 a         6854 c         5439 g         6063 t
ORIGIN      
        1 ctttcccttg ttgacctaat tcttgttgcc gtcggttcgc tcgaacctcc aattccgcat
       61 cgagaaaaaa cttcacatca gcactaggga acactcgcgt ccccatatct cttccttccg
      121 caataatccc aacttcacca acaacttttc gctgaatcgg gagtaaccag tccctgacgg
      181 actgaatagc tgcaatgcag gaagccaact ggctaatgtc tggcgttctt atgactcctt
      241 ggttaatggc ctgtccatcg accaaaatgg aaagggcatc cttactcagc atcagtcgca
      301 aattggtccg cgctagaagt ccttgaatgt gagcaagatt attcgggtca agctgttctt
      361 gttgaacctt ccaggcaatc gctcgataca aagcccccgt atccaggtaa acatatccga
      421 gacgggtagc caaacacttg gctacagtgc tcttgccgac tccagctgga ccgtcaatag
      481 tgatcaataa ccgacgacgt tgaacggccc tgttctcctc ttacgaccta atttctgaag
      541 cattataatg gtcgctcaga atttgtcaat aaatccaaca tcttatcttt gaaacccgga
      601 aaggatgtct caacgcatgc cacatcctcg atggtaatcg gagactccgc cactaatccg
      661 caaatggcca gcgacattac aacccgatga tcgccataac tctgacaaac cgcgccctta
      721 atctgggaac cgccattgat gattaaacca tccggttgtt cctcaacttc gacatggaga
      781 cgggataatt ctgtcgccat agctcgaatg cgatcggtct ctttcacgcg aagttcctgt
      841 gctccggtaa tgcgtgtctg gccatgggcc aaagcggcag ccacacacaa tataggcaat
      901 tcatcaatcg ttttgggaat gagttcgggg ccgatggtca ttcccctcag cggagcagac
      961 cgcacacgga tatccccaac cggttccccc gatatttctc gttggttgac gatggtgata
     1021 tcagctccca tctcgaggag aatatccaga atgcccgttc gttccggatt caatcctata
     1081 tcagtgagca ggatatccga atcgggaacg atcgatgccg caaccataaa aaacgccgca
     1141 gccgagatat ccccaggaac ctcaatcggc tttccatcaa aagagggtcg gccttgaagt
     1201 tgcacggtgg acccctcaac ctggagggga actcccaaat aggataagag acgctccgta
     1261 tgatcccggg atcgtctagg ctccttcaca atggtcgtcc cttcggcaaa gagcccggcc
     1321 aagaggacgc acgatttaat ttgggcactt gcaacaggag attcgtagag aatccccttg
     1381 agcccggttc cttgaatagc caaaggagcc aactcaccgg cacgtcttcc caagatgacg
     1441 gcccccattt gacggagagg agtgaccacc cggcccatcg gacgacttct cagagaggaa
     1501 tccccagtca gaacggaaaa gaaatgttgc ccggctaaga cgccagccaa aaggcgcaaa
     1561 cccgtcccgg aatttccaca atccaacaca ttcgaaggtt cctgcaggcc ccagagtcct
     1621 ttgccggtca cctcaagtcc ctcaggcgtt tcctgtacac cagcgcctaa ggcacgaatc
     1681 gcattaaggg tattcaggca atcctcgcct ttagagtagc ccacaatgcg tgtttggccc
     1741 tgcgccaagg ctccaaagat gagggcgcga tgcgtaatgg atttgtcccc cggtaccgac
     1801 aggctgccac gaaggggttt tttggtcgga ataatggtga actgactcat atcaaccgtt
     1861 ctcgtgaggc tttggcccgt gcaatcgcct cgtacaatcc aggtgcatcc cttttgctca
     1921 acaactcccg aaaattttgt agctgttgaa tataccgatc aaccatctcg accaaattcc
     1981 catgattggc cacacaaata tcccgccaca tttcaggaga actggccgca atcctcgtgg
     2041 tatcgcgcaa ccctccgcca gaaaaattca gcaagtccgc cggctgagat gttcgtcctt
     2101 gcaatttctc caaggtgtgc atgagagaaa aagccgcaat atgcggcaaa tgactcactg
     2161 caccgaagac ccaatcgtgg tccatgggat ccatcaacgc caccacagat ccgacagttt
     2221 cccaaaggtg acgaaccctt tccagggcct gggcattcgt atggggagtt ggagtcagaa
     2281 tacatcgagc gccttgaaac aaatgcgaat cggcgtgcgc cacccccgat ttttctttcc
     2341 cggcaatcgg atgggcgccg ataaaaaatg tcgagggagg caaccgagct tcaatttttg
     2401 agaccagttg gcccttcacg cttcccacat cactcacgat ggcggacggg gccagatctt
     2461 ttccccacag gtcaatttgg gagagatagg tctctacagg agtggccagg acaattaaat
     2521 ccgattggct caccgcccaa tgtggctcca gggcaaactg atcaatggcg cccattcgaa
     2581 cggctagttc gagattttct tgtcgacgac cgatccccac gaccgatttg gccaggccct
     2641 gttccttcag ggcaaggccg agagagccac caagcaggcc tatcccgata atggagacgt
     2701 gtcggaacaa cggttctgag tgggaagtcg acatgatgga aggcaaaagt gtcggaatca
     2761 caaaagaaca atcaaagttc ccggccgaca gcctgcgcca ccttttgtaa atcattcatg
     2821 agttcggcaa accggctggg cttcaacgat tcctctccgt cgcacaaggc acattccgga
     2881 ttagaatgga cttcaatcaa caagccgtca gctccagccg caattgcggc tttagccata
     2941 ggggccacaa gcttccaatt cccggttgcg tgacttggat caacaataac gggcaaatgc
     3001 gtcatttctt ttagggtagg caccgcactt aaatcgagcg tattgcgata ctgaggctca
     3061 aaggtgcgaa ttcccctttc acacagcatg acattccgat tgccacggga cataatatat
     3121 tccgcggata agagtaactc tttaatcgtg gccgacagcc cacgcttcaa caagacgggt
     3181 ttgtcatagg ccccgacctc ttttaacagt tcaaaatttt gcatattccg ggcgccaact
     3241 tggatcacat ccgctttttc caaaaaatgt ccgagatcac gagcatccaa aatttcactc
     3301 accaccggaa ggccggtttg tctcctggcc tcagccaaaa actccaatcc ttcctgtccc
     3361 aacccctgaa aggaataggg cgatgtacga ggcttatacg cgccgccacg caaaatggtg
     3421 gcaccggccc tagccacttc ttgagcgatg cccaccgtaa tttctaattt ctccaccgcg
     3481 caaggaccgg ccataatcac gatccgactt cccccgactt tcacgccggc tacgtcgatg
     3541 acggaatcgt gtttttgaaa ttcacggctc actaacttcc agggtgctag aatcggagtc
     3601 acactttcca cgccgggaaa cacgctcaag ggttgccctt gtagtacccg gtcatcgcca
     3661 agcaccgcca caatggtccg ttctttcccc tttgtgattt gcgtgaccat ccctaacgaa
     3721 cgaagacgat caatcaagtg atcaacattc tgctctgtgg cttcaggttt taagacgata
     3781 accattaatt aaatctgaat ccttgcgcta cgacagcgtg ggaagcactc gggccaacga
     3841 ttcaagaaag cgccgatttt cggtcggttg cccaatggtc actcggagca tgggcccacg
     3901 aatatgccgg acgatgacgc cttcccgaag caacgcgtca tacgcacgcc ttccatcaat
     3961 ccctacattg aaatacaaaa agttggcttg actgggaacg ggctctaaac ccatggtgat
     4021 gagcctttct tccagaaacg tcatttcggc ttcattcata gcccggcttt tggccacatg
     4081 atcgtcatcg gacagggctg cacgtgcggc ttcttgagcc aagctattca cgttgaaagg
     4141 gggacgaaca cgattcacat atccggcgat ttctttggtc gtatacccat aacccacgcg
     4201 caaccctgcc aacccataaa tttttgaaaa cgtccgtagt accacaaccg gccgcccatc
     4261 acgaacatac tgcaagctgt ccggaaaatc gggatgtcgc acgtattcat agtacgcctc
     4321 atcaaatacg acgaccacat gatcgggaag tcgggaaagg aagccatcga tttcccgtgc
     4381 cgtcagcatg gtgcccgtgg gattattcgg attgcagaga aaaaagagcc gcgtgcggtc
     4441 ggtgaccgcg tcaaccatgg ccgctatatc atggacccaa ttttttaagg gcacttctac
     4501 cggcacacca tgccctccca acacagaaag cttgtacatt acaaacgtca gatcggccat
     4561 tactgcttca tcaccaggag agagaaacgc gcgaaccaaa agactaatga tttcatctga
     4621 cccattgccc accagaacct gatcatcgga aaccttccac cgctcagcta acgcttgccg
     4681 aaggtagtga gccccgccat ccggataccg gtgaagcgac gcggctgccc ccgctaaaac
     4741 ctgtaacgcg ttgggcgaag gaccccaagg attttcatta gaggccagtt taatggcctg
     4801 agtaattccc agttcccgct ccaattcgtc caagggtttc cctggagaat atggggttaa
     4861 cgaggcgatt gtgggatgga cacgcagtgg catgagttta cattctttct tttgtgcgca
     4921 tgcggggaaa atcgaaaaaa cttcatatct accgagaaca atctattgcc ccagcctatt
     4981 ccggatcgac atccttcaag agcatacagg ttggaccaga ctctactgat gagaagaata
     5041 cgatcccaga accttcatca tcaaacaacg agacctgact tcctccaggg ctttcttcag
     5101 gcggtcatcg tcctgatgtc cctccaaatc catataaaac agatattccc agacttttcg
     5161 tcgggtaggc cgtgattcaa ttttggtcat actaatgccg tgcgatgaaa acgggcggat
     5221 caggtcatac aaggctccaa cccggtcttt tgctgagaca atgatcgacg ttttatcacg
     5281 accggttggt tcagctccct tttgggataa aatgaggaac ctggtgaaat tattaatgtt
     5341 atcttcaatt cgtgatcgca gaatattcaa cccatacaac tgagcagcta attcggaggc
     5401 aatggccccg gcgtttggat cattcacgca ccgctcagca gcccgagccg tactgggctc
     5461 ttccgcaaaa ttcacagaag gcaaattggt ttccagccag tttcggcatt gggcaagggc
     5521 atggggatga gaatagaccg tcttgatttc ttctaatttt tccgccttcg aaagaaagtg
     5581 atgggaaact tccagcatca cttcaccata aatcaacaag ggtgagtcca cgaacagatc
     5641 taacgtatgg ttgaccaccc cttccgttga attttctatg ggaaccacgc caaaccgcat
     5701 acgtccccgt tccacttcat caaacacatc cttaatgcta ttgaccgaca catagtcggc
     5761 cgatcccccg aacttccgca tggcagccag atgcgtaaac gtggcaggtg gccctaaata
     5821 cgcgaccgtt tgtgttccct ctagagaaag ggacgccgac ataatttccc ggtaaacatg
     5881 acgcagcgct tcactaggaa acggcccagg attcttgcgc atcaaccgtt ccacgatcgc
     5941 aacttcccga ccaggcgtgt gaagatgggc agacgaatcc tgttgttttt tcaaattccc
     6001 aattttaatg acgtattggg atcgctcatt caatatgttg agaatgtcgt catcaagccg
     6061 gtcgatctct tgacggcatt gttgcatatc ctctggaagc tccatgagac tcatccttta
     6121 tcctgaaaaa tctgcaattt aggcaggagc atacaaggaa taccctgctg tggcaagcaa
     6181 gcgtttcccc ctgtcagctt gcgactttcc ttcataaaca ggggcacatt tcctaaccac
     6241 aaacgctagg gcttctccgt tcagcgcggc accagaaata catatcgttg aaggggatca
     6301 ttcaaacaac gatccgtctc tttgattgtc caatcgggaa aggtggcctc catccagtcc
     6361 aaagtcattg aggaatcccc gtatgtgacg tcaccgtcga tcggcgccca gttgtgagga
     6421 ttgaaggcga agccctcctg tcggtgtcgg acggcaaggg catctttctg atcctgtgac
     6481 gcatgagggg cctgctccca gtattcgacc ggacgaatgg tcatggccaa cagaccatca
     6541 tccgcaatgt gctgccgcaa tgtgtgcaac gacgtcgtca tggccctgcg ggacaggtgg
     6601 gtgaacacgg agaacgcata gatgagatca aacttcaccc cccccaccgg caaggataca
     6661 gggaggtaat ctgacaccaa aaaatttgta ccgagcctcg actcccggca tagctctatt
     6721 gactcttccc gggggtcgac cccgaatatt ttgtcttcga cagtaaaata atacagcaac
     6781 tgggcgatac ggccatagcc gcacccaaaa tccaagacag tggcatcatc cagagaccgc
     6841 cctgtcacgc gcgcatagtt gtaactcaac gcacgcacaa acgccgtagt ttccttgaga
     6901 agtgccaagc catggtttcc cgtccagcct cgctgaactt cgtcactcgc catggcagga
     6961 aggagtcgtg aaagcttggg gtactcagga tcgggcatag acagcaatac caatccgaat
     7021 tcatcgagac tgagtcgtcg aagaccagtg atgacatccc ggcgagtcgt cactccatag
     7081 tcattttcca atgcacgcat aatctctttg gcatatccca gcatatagcc ctcagtcgaa
     7141 ttccaactta cagacagtga gaacctagcc ggaagaccct tcaccaactt tagggcatac
     7201 tgtttgaacc tttttttcat gaacaccttc accaatcgtt ggaaattccc cgcattcaac
     7261 ttcctctgaa tgactagtct cttagtagga gaattttgcg atgaaatttt ttgcttagtt
     7321 gaatttttgc ctttggttgt agcccacttc aggatcatga ggacaatgct ctattatcat
     7381 tttgttctat ccactcgccg gctaccacga aaagcttcaa tttgatccaa agttagttca
     7441 gtcatattta gatccaatgc cagctaaatg cgtttttttt cggcataaga ttaatgaaga
     7501 ccctccctat gcgctcgaaa tgcttttatt caaattcgcc aagtgtgtgt tcagggcgac
     7561 ataccctttg caatcaataa tgaagtgtct atccgcaaac tgcaacggga gcccgacagc
     7621 cccgctgtaa gggactctgt tcgattctac aggtaaaaag gatttaccct ttgatttatg
     7681 gccgctcgtt gaggccaaca gatggatcat ttgatttcag gaaggtgatg aattcaaaca
     7741 ccttctgagt gacctcagaa ggtggacaga atgtgcaatt cgttctgcca aattttgagg
     7801 agagtacggc gtgaaaattt tttaatgata actttcagca tcggtgggga attttccgca
     7861 ttcaacttct tctttatacc gccggagcgc ctgcaaggca tccgccttca gatgggcata
     7921 gggtttgaca aatttcggga caaagtcatc gaagaggcct aacaggtcgt aaagcaccaa
     7981 cacttgcccg tcacacccct gcccggcgcc aataccaatg gtcggtattg aaagggcttc
     8041 agtgatgcgt tgtgccagct cagtcggcat acattccagg accacggcaa atgccccggc
     8101 ggcttccagt gccttggcat cactcacaat agtttccgct tcatcctgcg ctttcccctt
     8161 aaccgaatac ccgcccgctt gattcactga ctgtggtgtc atgcccacgt gacccataac
     8221 gggaatgcca aaacgcgtca tggcctccac ccgatcaatc actgatattc ccccttcaag
     8281 tttcacagcg gcggcccccg cttggagaaa gcgccccgca ttccgcacag catcttccac
     8341 gctggcctgg taagacataa acggcatatc tccaatcacc atggcccgtt ttgcggcctg
     8401 agccaccaac cgtgtgtgat agagcatgtc ttccatggtc accgataagg tattgggctt
     8461 accttgcacc accatcccaa gggaatcgcc cacaagaatg acgtccagcc cggcctgctc
     8521 cacaatcctc gtgaacagcg catcataggc ggtcacgacc gttaactttt tgccctgttt
     8581 cttctgtcgt gtaaattcag gaatcgtcat gtgcgccaac ttttgtatga cctgagggtt
     8641 atgtgccgac attccgtttc gctgattcca ccgtattctg aagcagcatg gcgatggtca
     8701 tcgggcctac accgcctgga acgggtgtga gccatcccgc tcgttctttg accaagtcat
     8761 aatctacatc accgactaat tttccatcgg ctaatcgatt gatccccaca tcaatcacga
     8821 tcgcccccgg tttcaccatc tctgcggtga cgaagagagg cttcccgatt gccgcaatca
     8881 cgatgtcggc ttcacgaact acggcgggaa gatcttgggt gcgtgaatga caaatggtga
     8941 cggtggcatg tcgatgtaag agcaacatgg ccacgggctt tcctacaata ttacttcgac
     9001 caatcactac ggctcgtttc ccggagatat cttgtccggt cgactcgatc atctgaataa
     9061 cccctttggg tgtacaaggg ataaacaccg ggtcgcctgt gactaaacgt ccgacattga
     9121 ctgggtgaaa tccgtcgaca tctttctccg gggagacggc ttgcaaaatt gcttgactgt
     9181 ccatgacggg cggcaggggg agctggacca ggatgccgtg aattcgagtg tcggcattca
     9241 gctggtggat caattgcaat aaggtttctt gggtcgtgga cgccggcaga cgatgttcct
     9301 gaggatagag cccggctttt tcacaggcta ttttcttgtt ccgcacatag acggccgagg
     9361 ccggatcgtc tcccaccaac acggctgcta atccgggctt aacccctgta tctttttcga
     9421 gaaggcgaac ctcttctgca atgctctctc gaatcgcctg ggctaatgct ttgccatcaa
     9481 ttatctgtgc gggcacgtcg actccttctc ccggagtgtt gaaaaatgcc gacatcggcg
     9541 ttctcgcccc ttggccgggc tctcgtactt cttcgtacgc tccgcccgtc caattggctg
     9601 cggccttgct ggtcaaacat ttttgaacac tccattaatt tgctctcggt tactccgatg
     9661 cggattaatt gcaaaatata tcattttttc aacagaccct tgcattgaaa tggttatgca
     9721 gctcattaag gtgatttacg atatcaggca gggaaaatct tcgtcaacga aatggctgcc
     9781 tcatccttcc ttgacaccct gcaaaatcct cacttaccat gccacttcgg cttttcctct
     9841 aggcatccct tgttgctctg atcgtcatca tcgtctcagc gaaagttcgg ttgtttccat
     9901 gaagccatat tgtcttactg cctccttcag gaatgctatc ctcaccagtc tgttgctgct
     9961 gagttgttgg acggcgatct atgccgaaga ggtgacgaat ttagcgttcc cgcaaagcat
    10021 ggttgccgat gaagggcagg tctatttcat cgccaatgcc aacggagacc ctggaacacg
    10081 tgaaaataag ggttttatta gtaaagtgac gactgagggt aagatgattg acctccactt
    10141 tatccaaggt gggcaaaagt cggtcacgtt gaactcaccg aaagggatgg cccttgtggg
    10201 ctcaaccctt tatgtggccg atctggatgt ggtcagagga tttgacaaaa acactggaca
    10261 atcagtcaat tctattccct tcacacaatt tcacagtcaa tcccttgccg ggctggtggc
    10321 cgatcctcac ggggggctct atgtctctga tgccgattcc aataccattt atcacattga
    10381 tgaccccgcc cacgatcata ccgtgaaggt ctttgcccag catgaaagct tatcccaccc
    10441 gcgtggactt tccattcatc ccaggaatgg gcatgtggtc ggtgtcggat gggaggatgg
    10501 gaagattttt gagattgacg aaaccgggac gattcacgag ttgtttgcca acaccttgtt
    10561 taccggtagt ttccataatt tagacggtat tgatttcgac aaatatggca ccatgtatgt
    10621 gtctgatttg acagcaggga aagtctggag aattttggcc aatcacaaaa aagaggtgat
    10681 tgcggagttt ttgttgtccc cttcgggaat cggaatcgac cgggtcaatc atgtgatcat
    10741 ggttccctat ctgtatgtga atggtgcaga gattaatggt cttgagcgtc cctctaatgc
    10801 taaccctaac aaaaaacccc gatcctggtc tgattatgga atgggctggc tcaagaaaga
    10861 tcccggcgaa tgagtaaatg cctctattgt catcaacgta aaggcaaacg ggcttgtccc
    10921 gcacttggag gcgtgatttg cagccaatgt tgcggcacca atcgcatcac aaacatcagt
    10981 tgcccgactt cctgcgtctt tctcgagagc aatgatgact atcagcaaaa acgtgttggg
    11041 aaccggtttg aactcgaacg gcgagggttc tatcgacaat tactggatct cgggggggaa
    11101 cgggcgactg aaatatttta tgtctttgag gctctagcct atcgattttt tcaagatcgg
    11161 cgtgatgcgg aagacgccga ggtgctggaa ggactccaaa gccttcgacg aagtttcagt
    11221 ctgatccaga taccagaagc cggattgccg gccttcggtg aagaattacg aaaagaattt
    11281 aaggtcttcg gcgaacgcca accattagaa cccacgctgg tcaccaacgt gttagaccaa
    11341 gcccatacct tcattcaaag attttccgga ccagggcttc gttcccatcg ctttttacat
    11401 gggttactcg gctatattcg tgaacgccat cccgatgtgg cagaacaact cgcccgccaa
    11461 accggtgcgg gagggcgcat cattattccc agtggatttg gctatgcacc tgaccacgct
    11521 tccaccgaac cggtcaattg atcggcatct tccatccaca tttctggcca ttctctgagt
    11581 tattgaatca ggtctatcgt catttcaatc cgctcgagag gattatcctt caggtcgcga
    11641 ggctggctca cgatcgtatc gatgacgtca agacctttca cgacttcccc aaacacggtg
    11701 tactggccat ccaaatgagg agccttatcc gccatgatga aaaactgcga tcccgcgcta
    11761 ttgggatctg ccgtgcgggc tgcggaaaga atgccttttt cgtgaataat ccgattaaat
    11821 tctgctggta ccgtatagcc ggggcccccg gttccgtatc ggcttcgatt ggctggatct
    11881 ttggtcaaag gatctccccc ttgaatcata aatccaggaa taatccgatg aaaaatggtt
    11941 ccattataaa accctgactt ggctaatttg agaaaacttt caacatgctt gggtgctaaa
    12001 tcaggaaata acacggcttc catttctcca aatttcgtct taatcagaac gtgaggtgcc
    12061 ttggtctcag cctgcgatgg ttcttccgct gccgcctcgt ttgcgacagg agaaccaccg
    12121 gcagtgacaa ttgtgaacag tccgatcacc atcatgagct gacgtagacc gctggctaaa
    12181 gtccgtggct gcaacacacc cctcataatt cctcctccat ggctaatctg ttgatttctt
    12241 gattgaatga tctggctcgt ctaaaacttc aagcccagcc tgtgccatcg cttgcgctgc
    12301 cgcagcttgt tcggcctctt ttttcgtccg accctctccc tgacccatga tttttccttg
    12361 gatttccact gtgaccgcaa aaactttttg atgatccggg ccggattccc gaacaagtcg
    12421 atactgggga cttgctccaa attgtttatg cgtccattct tgcagacggc ttttcccatc
    12481 ccaccccact gaggacacag gactgttgtg taatgaagaa aattccgcgg tcaaaacttt
    12541 ttgaataaag gctctcgagg catccaaccc cccatcaaga tacaccgccc caatgatcgc
    12601 ttcaagggca ttggctaaca gcgatatttt ttcccggccc ttggtggctt cctctccccg
    12661 acccaatcgt aaccactgcc ccaactggag gcgactcgct gctttcgaaa gagtggatcg
    12721 gctaattaac cccgctttaa tcttggagag ttcgccctct gtggaatgcg gaaacatcgc
    12781 cgcaagactt tcgctcacaa ccaatcccaa caccgcatcc cctaaaaatt ctaaccgttc
    12841 attgtgggga ctcaccgcct ggggttgagt ttgagaaaat gaacggtggg tgagcgcctc
    12901 acatagcagg gcttgctgct taaattcata ctccaacgat tcctgaatgt cttcaaatgc
    12961 aagagcagcc acgagaccta caaagaattg ggagtgttga cacgcatgat cggtattctc
    13021 ttatctcttg agcaaaacgt tgtagtatat catatcgttt cgaggcaagg tcggggcaac
    13081 tgcttctgaa gtcctgagag gcacatcttt cgaaaccatc taatcaggaa gggactatgc
    13141 tgcctattcg cctttccaac gcctgaaaac cagacaggca ttgaccccac caaatccaaa
    13201 tgcattggat atggcgacat cgatgtcaca gggtctggca tgagtcggaa tataatctaa
    13261 gtcgcattcg ggatctggtg tctccaaatt gatcgtcgga ggaatgatgc catggtgcat
    13321 ggccataata gaaaagaccg cttcaattcc cccggcagcc cccaatagat ggccggtcat
    13381 ggacttggta gaacccacag gaatggaata ggcccggtcc ccaaagacgt gtttgatgac
    13441 ttgcgtttca atccgatcgg caaacgtgga tgtggcatga gcattaatat atcccacaga
    13501 ttctttggcg atccccgcat cctgaatcgc acgctccata caggtcaccg cacccgcccc
    13561 atcatctggt ggagcggtaa tatgaaaggc atcgctattc atggcatagc cgatcacctc
    13621 cgcataaatg tgcgcgcctc gagatttggc gtgttccaat tcctccagga ttaacacacc
    13681 ggcaccttcg ccaatgacaa acccgtcgcg gtctttatca aaaggccgac tggcccgttc
    13741 cggttcatca tttctcctgg acaacgcctt cgccgcggca aatccagcaa ccgctgtagg
    13801 gcaaatggtc gattccgctc ctccaaccag catcacatcg gcctctcccc gctgaatcag
    13861 tcgaaacccg tccccaatac aatgattccc tgtggcacag gccgtgaccg tacaggcatt
    13921 cggaccctta gcaccaaaac ggatcgctac ctgccccgag gccaaattaa taatgaccat
    13981 cgggataaaa aacggggaaa cccggtcggg gccgcgttcc agcaacacct tatgataatg
    14041 ttcaatggcc ggaagacccc caatgcctgc tccaatatag acccccacac ggtccgcctc
    14101 atccgggttt acgacaagct tggcatcatc caccgcctct tgactggcgg caatggcaaa
    14161 atggataaag gtatccatct ttttgatttc ttttttctca ataaacgttg agggatcaaa
    14221 gttcttgacc tcccccgcaa tctgcaccga atactgactg gcatcaaatc gggtaatggg
    14281 tccgattccg gattggcctt tacataattt atcccatgtg ttggatacgc caatatccag
    14341 cggcgtaacc aatcccaatc ctgtcaccac cactcgtcgt ggtctccatt cagtcattga
    14401 ggaactccat acatatcatg gctggaaaac ccagaaaatg cacctgaggc ttaacctcct
    14461 cgacgcccgt gatcaatttt ggacgaaccg caacttcgca cagtactcga tggccttctc
    14521 agactttttc cttaataaaa tcgatggcgg ccgagaccgt ttgaattttt tccgcatctt
    14581 catccggaat ttcgatttca aactcttcct ctaaggccat caccaattcc accgtatcca
    14641 gggaatcagc tccaagatct tccacaaact tggcctcagg gaccacgtca tcttcttcaa
    14701 cgcctaattg ttccacaata atttttttta cacgctcttc aactgccatt gttggctcct
    14761 cctccttgca aagtttatcg aatgacacat tctgttgcgc atgggctagg acgccatatg
    14821 catcccgcca ttcacatgta acacatgccc ggtaatatac ccggccttct ctgaactcag
    14881 aaaacacact gcgtcggcaa catccgaagg ttgccctaac cgctttaatg caatttgatt
    14941 cagtaacccc tctttaattt ctggcgaaag atgttgggtc atcgccgtat caataaatcc
    15001 cggggccacg gcattgactg taatattccg actggcatac tcccgggcaa ttgttttagt
    15061 gagaccaatc acccccgcct ttgatgctgc ataattggcc tggcccatgt tcccgatcgc
    15121 tccgacaata gaagcaatat tgacaatacg accacttcgc tgcttgctca tggaaggcag
    15181 aacggccttt gcacagaaaa acgtgccggt caaattgacc tggaggacgg caagccaatc
    15241 ctcctctttc atccgcatca gcaagccatc tcgcgtaatc cctgcgttat tgaccaagat
    15301 atccactcgc ccccattctt tgaggacgcg ttccatcatt cccttggcct gttcccactc
    15361 gccaacattg accgaaaccg ctatcgcttt gcgacccatt tgctggacaa gatccacggt
    15421 ttcttgacaa cgttcggcat caagatcggc caccaccaca tctgcacctt ccttggccaa
    15481 ggtggtcgca atcgcctgcc cgattccttg ggcaccaccc gtcacgactg caattttttc
    15541 cgtcaacaac attgctcaaa tccttcttga aaattggacc ccgtttgtcc ttaagcgcag
    15601 tgcgacagga cgtgagcgta ggattcctca tccatcacat tccacgtggt agcctcagga
    15661 acaatgcgct ttactaatcc cgtcaaaact ttccctggac cgatttccac gaaatgtgta
    15721 atacccagca gcgccatggc ctggatggtt tcctgccacc gcacagggga aggtaattgt
    15781 cgaacaaggg actcacgaac ctcctccgcc gacaacaagg ctttcgcttc cacattatta
    15841 accaacggta cctttaaagc agaccacttc agagaatcaa tatctttttt cagcctgtca
    15901 gcagcaacct gcatcaaagg agtatgaacg gggacactga ccggaagcgg catgacccgc
    15961 ttcgctccac gcgtcttagc caattctgcg gcatgctcca ctgcggcctt ttctccggca
    16021 atgaccgttt ggcctggaga attgaaattg gctggagcca caatgcccat agatcctgct
    16081 tcttgacaga cacttcggac atccttttcc gaaaggccta aaatcgcggc aactaacccg
    16141 cttccaggag tcacggcctc ggccatatac cgcgcacgct tctgcaccag attaaccgct
    16201 tctccaaaat tcagcgcgcc ggccgccact aatgcggcat attcgcccaa gctatgaccg
    16261 gcaaccgcag cggggagaag aggtccatta cggaccaatt gaaatgctgc gagactggtc
    16321 accagcaatg ccggctgcgt atattcggtt tgattgagtc gttctgacgg cccctcaaaa
    16381 cacaacgcgg cgatatcata tcccaagata tccgcggctt ccttatagag ggcctgaacc
    16441 gcaggactcc tatcaaaaag agtcttgccc atgccaacgg actgagagcc ttgcccagga
    16501 aaaagaaaac caaagactgg gttcatgctt ttcgctcacc ctctaaaaaa tgccatgatt
    16561 cagaggcagt ccaccacata gctcctctca cttcctgaaa ctagtcaaca ataaactctc
    16621 caccatccac acaaatcaca tttccagtga tccaatgggc tttcggatga cttaacagtc
    16681 ctagcgcatc ggcgacatct tgggtcgtgg tcagccggtt tgaaggattt tttcgtttgg
    16741 ctaaggtcag catttcatca gaaccgggta ttttacgcaa agccggagta tcggtgacac
    16801 ctgccatcag cgagtttacg gtgataccca gaggagccag ttccagggct aattgcctcg
    16861 tatgagattc cagggccgac tttgcagcgg aaaccgcccc ataggttcgc cagactcgtg
    16921 cgcctccggc gctggtcatg gagaagatgc gactccctgt tcccaaaaat ttttgtgcca
    16981 ctaaatcctg agtccaatac accagactat tagccatgac atccaacgtc atatccatct
    17041 gcgctttatt aatggcttct gcatgggatt ccgcaatata cggcttcagg gtcccaaaag
    17101 ctaacgaatg taacaacacc ctgacacaga ctggcttccc ggattcttcc gcccgttgct
    17161 tcagtgcctt caaaatgtcg ccacgctttt cgggatcggc cgcattgaca ttaaaaaaca
    17221 ggacctctcg gccgagtgaa cgaatctgcg aggcaatgcg ctccgcattc gcttgtgttg
    17281 attttcgatc aagatgcaca ccacagatat tcaaccctaa attggccagt tccatagctg
    17341 ccgcctctcc aaaaccacta gagactccta gaatcagcgc ccaatcattt tcaccccatg
    17401 aggtcgttcc agacataatt cccctctctt taaaccccca aaatcttctg gtttgctctt
    17461 gttggaaggc tcagattctt cctctgcttt tgtggcaatg atctcattca ggaaggagaa
    17521 atccgccggc agatacatta ccaacgaacg accgctgaag cccaggtaag acctgcacca
    17581 aatgcagcca acagcaccac actcccttta tgaattcttc cctcccggac agcttgatcg
    17641 agcgccaacg gaatcgatgc tgcagaggta ttaccaaatc gatccaaatt tatcatcaac
    17701 ttttctggag ccaaccccaa tcgctggccc acggcattca atatccgcat attggcctga
    17761 tgaggaatga atagatcgat atcgtccatt gctaatttat tggcctctaa ggcctctttg
    17821 gtggcctctt ccattgattt aacggcaatt ttaaacgttt cattgccctt catttttata
    17881 aaacatcgtt tttcttgaag aacctctttt gatgccggtt cccgcgaacc gcctccgggc
    17941 acctgaatca tatcagagag tccgccattg gcatgcaaat gagtggaaag gatccccgaa
    18001 ccttcagtgc cttgtcctag caccaccgcc ccagccccgt cgccaaataa gacgcacgta
    18061 ttgcgatcgg tccaatccgt tatcgccgac atgacttcgg aaccaatgac caatacgtgc
    18121 cgcataccat ttttcacata agcatctgca atagccaacg catagataaa cccacaacag
    18181 gcggcggaaa tatcacacgc cgctgcccga tttgctccta accgatgctg gatcagacag
    18241 gcagtggaag gaagtggaga atcaccagta caggtggcta aaaggatcag gtcgatctcc
    18301 gatggggcca catcagcagc gattaacgca cgctgagccg cctcaacagc taaatcggaa
    18361 caggcctgcc ccgggtcaac cactcggcgc tccctgatcc ctgtccgttc aataatccag
    18421 gtatccgagg tctcaaccat gcgctccaaa tccgcattgg tcaaaactcg cttgggaaca
    18481 taggcccctg tccctaaaat ccgactattc atacatgaaa caccgcgttg aactcacctg
    18541 catccttggg caatgtcacc tcggatttcc tcaatcaagc caccttccgc caaagcctgg
    18601 gctcgaagaa tagcattctt gattgccttc gaggaggaac gaccatgaca gatcatactg
    18661 ataccttcaa cccctaaaag tggggcccca ccgaattcgg cataatcggt ttttcgccgt
    18721 agccgcagaa ggggtccggc cacaaacaga taggaaaggc gacccagagc tgactgggca
    18781 atttctttca ttagcatttt ttttatcgca tccgccaacc cttcagaaat tttcagggcc
    18841 acatttccaa taaatccatc ggtcacaatg acatcggcat tcccactata gacatctcgc
    18901 ccttcaacat taccaatgaa attaataggg ctatccttca acaatttaaa ggtttctttg
    18961 gtaacttcat tccccttggt atcttcttcc ccaatactca atagacctac ccttggacga
    19021 tcggttccca ggagatgcct accatattca tgccccatta ttccaaactg gaagagttgt
    19081 cgggccgtac aatcaacggt ggcgcccaca tccagcatga ttgccttacc ctggcgagtc
    19141 ggcaacgtcg cggcgattgc cggtcgctct accccttgga tcaatcccaa aagaaaaaat
    19201 gctgacacca tcgtagcgcc cgtatttccg gcactcacca atgcgtcagc ttgcttattt
    19261 ttaactaatt ctgtggcttt ccaaatggaa gaatctcgtt tttttctggc aatcaacgca
    19321 ggagattcat gcatatccac aacctgtggc gcatgaaaaa tctcaatgcc ggctttggtg
    19381 ccgccgagtt tgtcaagatg agattggaga gagggttcgt ctccgaccag gataatcgag
    19441 agagaggatt cccttgtagc ctgtatggct ccttcgacat tgggagataa tccatgatcc
    19501 ccccccatcg cgtccacggc tattttcatg ggtttggtgt taaaagatga ctgactcgag
    19561 aaggaaaata ccgtttatcc ctcttcaacc gaaataatag cgatgccctt ataggttcca
    19621 caatttaaac atgtcgtatg agatggcttg ggctcatgac attgagggca cgtagacaaa
    19681 tttggcgggg tgagcttgag atgagatcgc cttttatctc tacgggcttt agaaattttg
    19741 tgtttaggat ttgccataat agtccaactt ttcagttaag aggtgactga tgccttcacg
    19801 atcctcttcg aagagaaaca gaggagcgct tggatggatt tttttttgat tttatttgct
    19861 ggaaatttgg cactggtgct gagctagtca ctggggtaca acacccacag atgccatcat
    19921 ttaaattgct cccgcatact tggcacaacc cgaggcagtt ttcattacat aaagattgaa
    19981 ggggggtagc aagaataacc tgctctcgaa tagccggaag aaggtcgatg tcatttcctg
    20041 taatagggta aacatcttcc tctgtcgcat tctcatcgac ggatttgtca cgcccctttc
    20101 ccccttttcc tttatccctg tgcggcacaa tcacattgac cgctttcacg gcctttttaa
    20161 attccacttc acacgacagg gataaatctt cctcaaattg atccaggcaa cgcacacata
    20221 ccctggccat ccttccagat attttccctt gaaaatatgc tgaccgctcc ccggtcagaa
    20281 aaagatctcc atgacaactt aagttcccaa tagggtttcc ctcatcatgg gagagcaata
    20341 agtccgaggg cttcacctca tgatgaattt gaagacctgt ggggggaaca tcaagtagat
    20401 tgatggttat agccatttaa caaccacacc tttctacctg ccccatttcg aagaatactg
    20461 atgatctcag tgttcttctg caaatgaaag gatagccaca tgcctggcac gcttaatcgc
    20521 ttgggttaat tttctttgat gccgcaagca atttcccgaa gtccgacgag gaataattct
    20581 tccgcgttcc gtgagaaaat tcctcaataa atttgcatcc ttgtaatcca atggcgtctt
    20641 atcgaagcaa aagcggcaca ctcttttccg ctgaaacaat cgtccttttt ccacagaaac
    20701 ctctcctagg ctaaaaatta gggcatgggg tctggataat actcttccgg atcataagtg
    20761 gactcctcac tcactcctgc cccagaggta cccgaggagg actttttcgg caagaacgtt
    20821 actgtttgag caacgacttc atgcttgctt ctcttctgcc cgtcgtccgt ttcccaccgc
    20881 cgttgttgta ggcgaccatc aacgatggcc ccgtcacctt tattgaggta ctgaccacaa
    20941 tgttcagctt gtcgtccaaa cacgacaata tcaacataac atacctcttc tttttgatca
    21001 tcggcctgac gatatcgacg gttgaccgct aacgggaaat taaccacagg ggtgccgctg
    21061 ggtgtatacc gaagctcggg atctctagtc agatttccaa tcaatatgac tttattgaat
    21121 cccaccatct tctgcatcct ccacacttgc ttcacctacc gctaccaaat cacttgggtt
    21181 cagccggacc gtcataaact tcatcacctg atcttccata cgctgaaagc gctccaactc
    21241 aaaaacgacc gtaggggggc cttcaaattg aaccgtaata taggtcccct ttcgctcatg
    21301 ttgaatatca taggccagct tctttttccc gctattatcc aactttaaaa tattcgctcc
    21361 cgcctgattc aggacttcct tcattttttc tatcactcgc tcattttctg tttccgcttg
    21421 aacgggacga agaatacaca gtgattcata cagtttcata cgtaaccttt ccctctgaca
    21481 gtatttttct agttttcagg cagagcaatc gtgttgtatc acaaaagggg acacaagcta
    21541 ccatagggta aattggagtg tcaaactcct ctcaggaata gaacctgcca attcagaaac
    21601 taaaagcctg ccatggtttc tcagtgaaga tgggctgttt ggcatttaca agcaagcggc
    21661 agacggtcga attctgatat caccacccaa aacggtcaac aggtccctat ctaaaagaga
    21721 aggcacttta taaactgtcg aaaattcact caccccttca tctaatctca atgtccacat
    21781 gaatgctaat tcatgtcagc gagattacat ccgctcacat tcgaaacttt ttgaggcacc
    21841 aaggtcattt acctaatgca aagagctggg aaatgagaga ggctgcctca gctctacccc
    21901 acgaattcaa cgggatgggt tgatccgctt ctgccaggcg gaatggcata cacaaatatt
    21961 tacagacctg tctcagagga ccgcacatga aatcgattca tggcaatacc cggcccttca
    22021 caaataagac attccaatgc atcaacggct ttcggaagaa ttgagacaac ctgctcccac
    22081 tcttctgatg aaaatggtga aagcacaaaa ttcgcaaggc tctcattatt ctgaggccgt
    22141 cctatgccaa ccttaaccct ggagaattct tccgtttcca gacaggacaa aatagaccgc
    22201 agaccattat ggccaccagc gccacctcgc gttttgatct tgagcactcc caaagacagg
    22261 tccagatcat cgtgaacaac aataagatca ggcgaattgg gagacaactt ctgaagaaca
    22321 gaccgaaccg caaccccggc ttggttcatc cacgtaagtg gaagaagaag caaaaccgga
    22381 tagcccttaa cttcaccttg cccgagaaac ccatcctcct gcttggccca agtcacacgc
    22441 caacgatccg ccgcctgctc aagaaccatc cagcccaggt tgtgtcgcgt gtatctgtag
    22501 gactctcccg gatttccaag tcccaccacc acatgcaagg tctacttctt gccttccttc
    22561 gctttagtat ctgatgcggc cttcgtttta tctgccgccg ccacagcccc tccagtggca
    22621 gccacctctg cagctttttc tgcagtggca acaggagcca ccccttcagc cacctcgcga
    22681 gccaagaggg actctagctt cgcttcagac atcttcgtgg caacatgggc aaccatgaga
    22741 tcctcgtcat ccaaaatttt gagacctgtc gggaccgtaa cttctttcac atgcatacca
    22801 tcaccaatcc caaatcctga cgcatcgatt tcaatatgat ccggaatttg agccggtaaa
    22861 cactcaatat gaagttcgcg catcacctga tgcaaaatac ccccctcttt cacaccaaca
    22921 gggacttctc ctgtaatcgt cacggggact ttaacgcgaa tagctttttt catcgagact
    22981 tcaaagaggt ccacatgtag aatcgccccg gtaatgggat caatttggtg gtcttgaagg
    23041 acggcgatcc gttcttgccc cccggaaatc cgaacagtca gcaacccagt gtggccaacc
    23101 tgagaaatca ccagattgcg ggccgtcttg tggtccatgg ccacaaattc agacttccct
    23161 ccgccgtata gaactccagg aattttccct tgtcgccgta gttgccgcgc cacgcctttt
    23221 ccagcctgat cccgtttttc aacttcaagt tcatatttca tcgtcttgcc cttttctaaa
    23281 tgagagtgaa tgaacagtta gctaatttat acaaacaggg atgtcaccga ctcttcacga
    23341 tgaatcctca aaatcgcctc acccaacaaa ggagcaacgg tgagagtctt taattttgga
    23401 cattgctgat ccttcccatt taaaggaatg gtgtttgtca ccattacctc acggagacaa
    23461 gacgattgca acctctccag tgctggacca gacaggactg catgggtact cccagcccaa
    23521 gccgacaacg ctccgttatc catacaggtt tgagctgtct ggacaatggt tcccgccgta
    23581 tcaatcatgt catcaaacaa taatgcatgc ttatcctgaa catcaccaat aatattcatc
    23641 acttcagcct gattcggcga ttcccgccgc ttatcgataa tcgcgagaga ggaattcagt
    23701 cgtttggcaa atgctctcgc tcgctcgacc cctcccgcat caggggaaac gatcaccaaa
    23761 tcagaaacat tttgcttttt aatatagtcc aacaacacag gcatggcata aaggtgatca
    23821 acaggaatat tgaagaatcc ttgaatcggt ccggcatgaa gatccatggt tagaatccga
    23881 ttcgcgccgg ccgcggtgat caaatctgca attaatttag cactaatagg cactcggggt
    23941 tgatcttttc gatcttgcct cccatatccg taataaggaa tgaccgccgt gatcctggaa
    24001 gctgatgatc gcttgaccgc atcgatcagg agcaacaact ccataatcga agtattcacc
    24061 gggtggcagc aggattgaat cagaaaaaca tcgcctcccc gcacattctc atcaattcta
    24121 accctgatct ccccatcact aaaggttgta accgttgcct gactcaaatc ctgcccaata
    24181 tactgggcaa tttctctggc caacgcaagg ttggagttac ccgtgatcaa ttttaaatct
    24241 ctgagcatgg tggtagtctc aacttgccta ggtgaacctg aaccgtagca gattttcagg
    24301 aaaaaaagca gagcaaaggt aattctacga ttggcggctt gtcaactcaa atcactgaat
    24361 ttacactttg gaaacctgag aagacgagcc aacatggaat gggtcatgag gtaaatcagt
    24421 cgaacccatc gggatatcaa agattttcca gctcgtatca tggcgcaatc gggtggaagc
    24481 ctgccgggct tcttcctgag tgcggaaaat cccaaatacg gtggctccac tacctgataa
    24541 tagtgcggct tgagccccaa aggaaagcaa agtgtcttta atgaatccca aaatcg
//
LOCUS       DATIAL010000293        23611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_699813, whole genome shotgun sequence.
ACCESSION   DATIAL010000293 DATIAL010000000
VERSION     DATIAL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 23611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23611
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699813"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2432
                     /locus_tag="VLA60_09130"
     CDS             <1..2432
                     /locus_tag="VLA60_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09566.1"
                     /translation="ANVIGFVQFGFSLKRLEAQRATFLQSLAWYTLSFVLLASLITIL
                     LVRKITAPVKKLAKVARDITQGNLDQQLEFTIDTKDEIHDLAVSFTQMFDWLRQYRRE
                     VESYQQSLEEKVAQRTHELRQAKDEALHLAEDAKAASQAKSEFLATMSHEIRTPMNGI
                     IGMTDLLLDTELAKDQRYYATTVRNSGESLLTILNDILDFSKIEAGKLEFETIPFDLR
                     QAVEETLELVGERALKKGLELVGWVFPDVHAAVMGDPGRFRQVLLNLLSNAIKFTEHG
                     EIGVQILRLEDGEHDIDIRVQVSDTGVGISPEAQKKIFESFSQADSSTTRKYGGTGLG
                     LAICKRIVELSGGTIGVESQLGQGSVFWFTLRLQKYWQDLPSHFDTDQTILEGLRVCC
                     VDDNDTNRHLLAQYAEDWGMRATSASSGMEALAVLRAGVAREKPFDLAILDMHMPNMD
                     GLELVRAIKADPRIQNVRCLLLTSLGRRGDAKEAQRAGFQAYLTKPVRKTQLKQNLVA
                     VMAPEQPTEMANQEILVTLHSLKDLSTNQQPSTILVADDHTVNQELASLLLHKLGHEV
                     EVVSNGKAAIEALQKRPFALILMDCQMPDMDGYATTKAIRAWEGEQRHTPIIAVTANA
                     MSGDREKCLAAGMDDYLTKPIKPDKLRDMLHRWLPREERTAPPLHHIDDGVRLRKPLT
                     NSDPLKTVSVETQPLDAERLAEWRALGGDEFVSKILKNFIREALECVSQVHEAVTTEN
                     WEDLTLAAHGLKGICRNVGVQKLSELALALEQHNRQESFETVRLKLSALQQELARVQY
                     ALEHEVVQHST"
     gene            complement(2649..2837)
                     /locus_tag="VLA60_09135"
     CDS             complement(2649..2837)
                     /locus_tag="VLA60_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09567.1"
                     /translation="MGRPTKVTQAKRNREMVKRMKQQDKEARREQRKSENEEPPRVLD
                     GEDPDLVGIRPGPQPPQY"
     gene            complement(3005..3274)
                     /locus_tag="VLA60_09140"
     CDS             complement(3005..3274)
                     /locus_tag="VLA60_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09568.1"
                     /translation="MKLTMKWGGKVRNRKTFKPEPKIRWDLLGLADPSKEKVPMPIDE
                     VRRQVLMMSSPGFDQDSATRLDVVPQKVSRSSTSGKPSKARREST"
     gene            complement(3651..4325)
                     /locus_tag="VLA60_09145"
     CDS             complement(3651..4325)
                     /locus_tag="VLA60_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09569.1"
                     /translation="MSACEGWSLPDRELTLSCGKGSHDIMILPANILKLSMILLLCWV
                     GGAWAGYEEGEEAYLQENFPAALSEWRPLAEEGNTEAQNMLGYMYRYGQGVPQDFEQA
                     RLWYRRAADLGNARAQNNLGAMYRQGLGVPQDYQEAFRWFLRAAEQGNGGAQNHVGLM
                     YYKGEGVRKDLVQAYMWAYLAAQQGMDPSIQALDLLSQEMTAAQISEATDLARKWKPK
                     GEEVAL"
     gene            complement(4340..6148)
                     /locus_tag="VLA60_09150"
     CDS             complement(4340..6148)
                     /locus_tag="VLA60_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HSF09570.1"
                     /translation="MSALIYGQKLTKAYGTKRLFQELTLGVSEKDRIGIIGPNGSGKS
                     TLLRIFAGLEQPDEGRVTRRQHLRIAYIPQQADFDSESTVAEEIERAALASGLHAHDC
                     VARVQETLGRMGFAQGDQLVRPLSGGWKKRLAIACGFVQAPDVMLVDEPTNHLDLEGM
                     RWLEQVMSQAPWPWVMVSHDRWFLGHATNKIAEINRRYPDGIFLVPGGYEEFLVKREE
                     FLNSQTQQAQALAGKVRREEEWLRRGPRARSTKAKSRIDSAHALQAELAETQVRLRQE
                     ETSIDFVGSGRRTKQLMVVHHLRKTFGPRTIIQKLDWVISPGTATGVLGHNGSGKSTL
                     LKLLAKELEPDQGSVTYADKLQVVYFDQNRDQLDPKMTLRRTLSDSGHTVMYRGQTVH
                     LAGWAKRFQFQPEQLDLPIELLSGGEQARAVIARLMLQSADVLIVDEPTNDLDIPTRE
                     VLEESLKEFPGALILVTHDRYMMEEVCTDFVGLDGQGGYGQFADYGQWESWLRRQRSG
                     KDQTGTQSKSVRATAERPKAKKLSFRDQQEYDMIEKRVLDAEAVLELCRTETENPKIA
                     ANHHKLQEAYENLKSAEQEVERLYTRWAELEAKQQA"
     gene            complement(6464..7630)
                     /locus_tag="VLA60_09155"
     CDS             complement(6464..7630)
                     /locus_tag="VLA60_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HSF09571.1"
                     /translation="MIVVGMGPAGASTAYELSRLGMSVVAFDKQVHPRYKICGGGLSA
                     RIERILPADFKEIVEGTVYRVQFIYGGTNSFIVESPRPVAYMVMRQTFDQWLVEKARQ
                     AGAEIREEESVVAIQELEDGVEVATGKGRYRGRVVIGADGAMSVVAQQCFPGREVGNI
                     PALESEYHGDTPHTFQRSQTVLISLRAAKKGYGWVFPKEKGLSVGVGEFIRGRNRPKR
                     SFEDFVRHESALAGLSIPSPLGHPLPIAHHPAYRPGQRWTGSLVRHRAVLVGDAGHLV
                     DPLLGEGIYYAVRSGQLAAASVASTIRDPRHRLEEYEKNAEAEFGLEFRVASRLGAII
                     YGLPRSCHWWAGRTFPDAYQRVLGRYCELLQGRETYQTLWAKILQRLKRPFGCS"
     gene            complement(8008..8346)
                     /locus_tag="VLA60_09160"
     CDS             complement(8008..8346)
                     /locus_tag="VLA60_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09572.1"
                     /translation="MIIKERNMNGLPVVSLSGKLDIFSKNAFKETAEKYTDSNSQGLI
                     LDFQGVTFLDSVGLGALVVLAKKFLKLKGRMIIVNPQDPVKTLLSEMHMEKHIPMYTT
                     DSEFSTFSEL"
     gene            complement(8720..9775)
                     /locus_tag="VLA60_09165"
     CDS             complement(8720..9775)
                     /locus_tag="VLA60_09165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HSF09573.1"
                     /translation="MTRYAGHSETLGVRMSDDRSILIGLVFFVGLGLFAGLGESSAMS
                     ISQAIEQAKVATVGILQDDQLEIPDNDYGLPVSIRGSGIHIGRGVIVTARHAVERTEG
                     GKVIIPDTIHVVTDDLFELPAARQGANAYLDVAVYQLQGAESDWPQAQVTFADEDVTY
                     GDQVFTVGYPLGWGPSINFGTTGNPNTFLPTVNSRLMQVDMSVCSGNSGGGLLNQHGQ
                     LVGLMHAIIQTETQHDDRRCARLAFVLPGPLVQRVVTAVLADRVPGFSVLGIQLQTLR
                     MGSRWALVVAKATGPSRHAGFQKGDVLVAINDVPITTPAQLKNYLIERTEPGQVVELR
                     VQRGDSQELLSVTLGRS"
     gene            complement(9939..10529)
                     /locus_tag="VLA60_09170"
     CDS             complement(9939..10529)
                     /locus_tag="VLA60_09170"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HSF09574.1"
                     /translation="MGETNDRGRMATSHPHSGLPDYLVPGLSILFVGINPGLRSAEVG
                     HHFAGPSNRFWKLLFDAKLIPLPMTYQDDWQLPEFGYGLTNLIARPTAGVQDLEKRDF
                     LEGHQALVTKITTYQPRLVALLGVSMARMLLSSKKATTAVQRLIENPIQVGLQSLALG
                     GAPVCVLPNPSGRNAHYSYQHMKALFGELKDWSQPA"
     gene            complement(10698..11348)
                     /locus_tag="VLA60_09175"
     CDS             complement(10698..11348)
                     /locus_tag="VLA60_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09575.1"
                     /translation="MTVTHRMLYILLATLLLSWWVIPGVWAEESSQVLTKQAMDECHE
                     GRRAKEETVRVAHFERGRKLAETAVGLDERSADAHFALFCNIGERMRANGEVLFSVFE
                     YGRMMEALDKTLLLNPNHMDALSSKGTILIEVPGLFGGDSEKGEAMLRRVIQQDPQSI
                     NARMVVARSCAQRGLDQEAFDLAKKALELARAGNRDDLLPEAEKTFSEIQTKLASK"
     gene            complement(11562..12806)
                     /locus_tag="VLA60_09180"
     CDS             complement(11562..12806)
                     /locus_tag="VLA60_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HSF09576.1"
                     /translation="MNIAVIGGGPAGLMAAEAAIAEGACVDLYDAMASVGRKFLLAGK
                     GGLNLTHSEPLERFLARYGARQGQFQSLLAGFGPAALREWAHELGVETFIGTSGRVFP
                     TDLKSAPLLRAWLRRLRQHGMAIHVRHRWGGWTETGSLRFATPQGDRFVSADAVVLAL
                     GGGSWSRFGSDGAWVPLLAGRGVAIEPLKPANCGFDVVWSPHLRGRYAGHPVKPVAVA
                     GTTQEGVEFRQQGEFVLTETGLEGGVIYTISAWMRDEIVKTGTALLRLDLAPDRDVSR
                     LISDLSRPRGSRSMASHLQRRVGISGVKAGLLRECVSKQDFADPVLLGAAIKALPVRL
                     VAPRPLEEAISTAGGVAFEALDQRLMIRTLPGVFCAGEMLDWEAPTGGYLLTACFASG
                     YAAGVGAARWLQSTRNPTLGTF"
     gene            13069..13236
                     /locus_tag="VLA60_09185"
     CDS             13069..13236
                     /locus_tag="VLA60_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09577.1"
                     /translation="MEQEEIRQLWADGEDWIIKRQHNQYFHRPDGKYGDWKPGLPPGV
                     VKPDVDTLFDN"
     gene            13337..13732
                     /locus_tag="VLA60_09190"
     CDS             13337..13732
                     /locus_tag="VLA60_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09578.1"
                     /translation="MRISGRIIGLLKEYMHDLVEQAKQEEAAHASFGFASQPYRADEA
                     ISDLLAILDDRIESEGVQVGLPIEFLHQMWTLCNQATTHISETVWLRKNLDQGTGSKA
                     EVRHLTYQALIAFLESQPEDDESGDATPN"
     gene            complement(13690..14559)
                     /locus_tag="VLA60_09195"
     CDS             complement(13690..14559)
                     /locus_tag="VLA60_09195"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HSF09579.1"
                     /translation="MDAAAFEITIPISVDAAELAGILDCQEFLGAWDAEGSTVLYWSR
                     NGAEILQQVRSAISVLGVVLPEGALRFHPVKAQDWNATWAASVQPIRIGRRIGIRPSW
                     ATMEMPEDGVELIIDPKQAFGTGHHATTQMILEWLEGVTWVPGMRVLDVGTGSGILAM
                     AALRLGATSALGIDVDTTALDCAREYAVVNNIQEALTLSSAQLATLPAQSFDVILANL
                     DRRTILHVIDQFARMRGPQTQLVVSGLLEEDESEIVRQFEGCGWRQRHVRGWDGWLAI
                     QFGVASPDSSSSG"
     gene            14793..16679
                     /locus_tag="VLA60_09200"
     CDS             14793..16679
                     /locus_tag="VLA60_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09580.1"
                     /translation="MKHTTTDRREQAILRLIQRTERMQAKGTIASRQFSRWRMGTFLA
                     GAAITIGVYQQAWFHTGNGLLLVFLIGFLTISWFHQRLKSRLHRLQLWLEIKKDSLAR
                     LQLDWPHISAYEHRAPERHPFAIDLDLTGPHSLLALIDTTFSSIGQHRVATWLFQSNL
                     PESDGLAWTTRQTLVKELTPLSRLRDRCLLANRLISSDRLNGTRIISLLEAPVSIRHL
                     PLIIFAACGLTSLTIVLGLWTLLMGAPNFWLLPLVFYIGTYFLLSGSIHPLFGRVLAL
                     HEELEKLVSVITELERRTFLHQPTMVQVCQSILTQQNRPTQSLKQLSRICQGLSVKAH
                     PLVHLGLNALIPWDLWFAGKLNRLISRLRTTLPAWLDTIGTVDAAGALARYAYLNPDY
                     LWPQLETTNTTPDTQGLTAGSLGHPLIARAHRVTNDLELRGEGHLLLVTGSNMSGKST
                     FLRTVGINLCLAQAGGPVCAKIFSWTWMRLYCCIRVTDALDEGLSYFYAEVKRLKVIL
                     EAVESTNPHPVLFLIDEIYRGTNNRERLAGSEAFVQALQQSRGLGMISTHDLELTGLA
                     SGNGHIRNAHFQETVEAGTLHFDYRLRPGPCPTTNALRIMAQEGLPVPHGFYSAGGTR
                     PDRL"
     gene            complement(16676..16951)
                     /locus_tag="VLA60_09205"
     CDS             complement(16676..16951)
                     /locus_tag="VLA60_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09581.1"
                     /translation="MPRPPFPTGPRPTPRMAGARPPAPPGTPKQFESLRASLLFCPKC
                     QTATPARERLLLVLPTGSLYEYLCAHCGTSTGSKTDQGQSEVRLIVP"
     gene            complement(17054..17374)
                     /locus_tag="VLA60_09210"
     CDS             complement(17054..17374)
                     /locus_tag="VLA60_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09582.1"
                     /translation="MAFFINPVPSGAADSPPVGDGVPLAPAFCGLCPERRQAPLRPHP
                     GFPPTKQARVHPYILGCLGYGAALVDDECNGTPFKVGRILFPSLTPPNPPPLAINAFF
                     DVFI"
     gene            complement(17903..18496)
                     /locus_tag="VLA60_09215"
     CDS             complement(17903..18496)
                     /locus_tag="VLA60_09215"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF09583.1"
                     /translation="MTSKSLTWATIIVTIVLVAVPNVFAIDKNSGIHVGIPPRTVADY
                     LRAVIMAHRHFYTIHIVNRLEKERIVDASENWQATHSLPLPVQLLQETSEMVELTSPN
                     VRYRLISQWPINKDNAPANEFERMALKEVQMHPNRPYSSTIGAGEGQVFEAVYADVAV
                     STVCVQCHNSHPNSPKSDFKIGDVIGGLLISFPLRSQ"
     gene            complement(18493..19317)
                     /gene="modA"
                     /locus_tag="VLA60_09220"
     CDS             complement(18493..19317)
                     /gene="modA"
                     /locus_tag="VLA60_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF09584.1"
                     /translation="MVIAGLKILIAAIAGLAAMVGTWEPVQAEPLTVGAPPSLRPAFN
                     DILPLFEREYGVAVNVVYTPSKTLLQQIEKGAPIDVFLSAGVEEVEYLYKKGLTLNGR
                     PRIYAQTSLVLVMSSNSPALFVSLHDALSNQSTRIALGDPQTSSLGDVTARVLTKFYP
                     TYKSRPHILYGAHSEDIMNLIRMGKADVGLVFRVDSISNGHIRISDENPIGAYVPILF
                     GQAVVWTCRDSLRSTAEQFSDFLMTPRIQKLLVLHGFDSIPLPVGLIPIEKKERTS"
     gene            complement(19746..20207)
                     /locus_tag="VLA60_09225"
     CDS             complement(19746..20207)
                     /locus_tag="VLA60_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09585.1"
                     /translation="MKFPFAFAARWVLCVLACLQLGLVGCHVTLISSYDPEMDRTATS
                     LQKRMDAFLTRLETHAGESQAHYSWDQVFYQDYLVELRSLRLRAQSDHVNEFAAKQLT
                     LMMDNFEQLRLAHEAGPLPVPTIQATRDLFNQGWQVIIAQEIAKRRGVDPP"
     gene            complement(20533..21543)
                     /locus_tag="VLA60_09230"
     CDS             complement(20533..21543)
                     /locus_tag="VLA60_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09586.1"
                     /translation="MTDQPGEKTNRRSMRSRFMVPGLTLALVGSIALLWIPVPSHTIL
                     LKAFYDFCHFPLFGGVAILLLYLARQLGEPRGWSVGRQYGTACIGALTLGALTEGVQS
                     FSSSRFAEWSDFCRDVSGAVAALGMYVTYDSRFTGRVATWRLAPRKHLVHAGVGLLVV
                     IALSPVVVWTYAYWDRATRFPSLVQFSSAWEMMFVKGRDCAVQVVPPPAGWEKSRVDT
                     VGHMVCSPAHYPGIRLEEPYPDWRGFSHFRVEVYSELPEARSLTVRIDDAPHNHGYAD
                     RFNRAVIISPGLNHLHILLDDIRHAPLGRELDLGAIKNVRLFAISPPEEFSLYVDNIR
                     LE"
     gene            complement(21554..>23611)
                     /locus_tag="VLA60_09235"
     CDS             complement(21554..>23611)
                     /locus_tag="VLA60_09235"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF09587.1"
                     /translation="PVPDHQLVQETLHDCLTEAMDLAGLRQVLERIENQEIQVRVVES
                     PVPSLFAHEILNANPYAFLDDAPLEERRARAVNLRRTSSPAFDGNIGTLDAAAIEAVV
                     EEARPLVRDADELFDVLQVLGWLPFAMGELWHELGEALLSCGRLLVLRVPVEGLPETK
                     VVEGWTTKEHLTRLQVLFPEAKVVAGLEDSRAVSVGDTSATAASAARDVVQGWMPHVG
                     PVTADELSRKLNVPALLVQQALLQLEGEGQVLRGHFRPSSRIDSSTGSSTKEEQRIGN
                     HAVEEEWCDRSLLARIHRRTVTSLRREIEPVAASDFMRFLFRWQHRAPGTQLHGEAGL
                     REVIHQLAGFEAPASAWEPSLFTARLAHYKPEWLDHLCLRGEVAWGRLTPPESKGTLL
                     PVVGTGQGIGNFVLDAGAAVPAFRPLTPTRLAPISFLRRQDVSWILGVAKPGTTAGQL
                     GGGLHLSGVAQAVWDCLDRRGACFFADLTGRTGHLPAEVEQALWELVATGLVTADGFD
                     PMRALLDPRRRRAEGKDRARRPGHSVGRWDLFQGDPNAHDEESQIRLHPHEQWARQLA
                     HRYGVVFRDLLKRESLPVTWRELLVQFRKLEWRGEMRGGRFVNGFTGEQFALPEAVES
                     LRAVRRDIQAGAQEIRLSAADPLNLIGIILPGERISAHTSKPIFFRNGVPSADTVSVV
                     SLA"
BASE COUNT         5977 a         6719 c         5711 g         5204 t
ORIGIN      
        1 aggcgaacgt cattggcttt gtgcaatttg gattcagtct caaaagatta gaggcgcaac
       61 gagccacctt tcttcagtca ctcgcctggt acaccttatc gttcgtcctc ctggccagtc
      121 tcattacgat tcttttggtt cggaaaatta ccgcacctgt caagaaactt gccaaggtcg
      181 caagggatat tacccaaggc aatctcgatc agcagcttga attcacgatc gacacgaagg
      241 atgaaattca tgatttggcc gtgtcgttca ctcaaatgtt tgattggttg cgccagtacc
      301 ggcgcgaagt ggaatcgtat caacaatctc tggaagaaaa agttgcgcaa cggacccacg
      361 agctcagaca ggccaaagac gaggcgcttc atttggccga ggacgccaaa gcggccagcc
      421 aggcaaaaag tgagtttctc gcgaccatga gccacgaaat ccgtaccccg atgaatggaa
      481 ttattggcat gactgattta ttactggaca ccgagttggc aaaggatcag cgatactatg
      541 ccactaccgt caggaattcg ggagaatccc tgctcacaat tctgaacgac attttagatt
      601 tttcgaaaat cgaagccgga aaactcgaat ttgaaaccat tccttttgat cttcgtcagg
      661 ccgtggagga gacgttggaa ctggtcgggg aacgagccct gaaaaaaggg ctggaattgg
      721 tgggatgggt ctttcccgat gtccatgctg ccgtcatggg agatcccgga cgatttcgtc
      781 aggttttact caaccttctg agcaatgcca tcaaattcac cgaacatggc gaaattggag
      841 tacagatcct gcgtctggaa gacggagagc acgacatcga tattcgcgtt caggtttcag
      901 ataccggcgt gggaatctcc ccggaagctc agaagaaaat atttgaatcc tttagccaag
      961 ccgatagctc tacgaccaga aaatacgggg gaacaggact gggactggct atttgtaaac
     1021 gcattgtgga gttatccggc ggtaccatag gagtggagag ccaattagga caaggaagtg
     1081 ttttctggtt tacgctccgt ctgcaaaaat actggcaaga cctaccgtct cactttgata
     1141 ccgatcaaac gattctggaa gggcttcggg tatgttgcgt ggatgacaat gataccaacc
     1201 ggcatctctt agcccagtat gccgaggatt ggggaatgcg agcgacttct gcttcttccg
     1261 gcatggaagc cttagcggtg cttcgagccg gtgtggcccg ggagaaacct ttcgatctgg
     1321 ccattctcga tatgcatatg cccaatatgg acgggctgga attagtcaga gccatcaagg
     1381 ccgaccccag aattcagaat gttcgctgcc tcctcttaac ctcgctcgga aggcgcggtg
     1441 atgccaaaga ggcgcaacgt gcgggatttc aggcgtacct gaccaaaccg gtccggaaga
     1501 cacaactgaa acaaaactta gtggctgtga tggcccccga acaacccaca gaaatggcga
     1561 atcaagaaat tttggttact ttgcattctc tcaaagacct atcaacgaat cagcagccga
     1621 gtaccatcct tgtggcagat gatcatacgg taaatcagga attggcttca ctcttattac
     1681 acaaacttgg gcatgaggtg gaagtggtat caaatggtaa ggccgcgatt gaagctcttc
     1741 agaaaaggcc gtttgccctc atcctgatgg attgtcagat gccggatatg gatggatatg
     1801 cgaccaccaa agcgattcga gcctgggaag gagaacaacg ccataccccg ataattgcgg
     1861 tgacggccaa cgccatgtcc ggggatcgag aaaaatgtct tgccgccggc atggatgatt
     1921 atttaaccaa acccattaaa ccggataaac tccgggacat gctccatcgc tggttaccac
     1981 gagaggaacg aacggcccct ccacttcatc atattgacga cggcgtacgc cttcgaaagc
     2041 ccctcacgaa ttccgatcct ctcaaaaccg tgtctgtcga aactcaacca cttgatgctg
     2101 agaggttggc cgaatggcgt gcgttggggg gggacgaatt tgtgagcaaa attctgaaaa
     2161 attttattcg cgaagccttg gaatgcgtct cgcaggttca tgaggctgtc acgacagaaa
     2221 attgggagga tttaactctg gcggcccatg ggctcaaggg aatctgcagg aacgtcggag
     2281 tgcaaaaatt gtccgagttg gctttggcat tggagcaaca caatcgtcaa gaatcgtttg
     2341 agacggtccg gctcaaatta tctgctctcc agcaagagtt ggctcgagta caatatgcct
     2401 tggagcatga agtggtccaa cactctacat aaaatttttg tagttgcatg ccaccctcag
     2461 atggacggaa acaggacagg aggtacgggc aatggggcta ttcaataaga tagcctggca
     2521 ggaacgaaga gggagacagc tatttcagat aatgccaaac ttgacaggac acgctaggat
     2581 tgggaaaagc atcccaagaa gaaaaccggt acttggatca gacgatcagt gagaaattgt
     2641 aggactcact agtattgcgg tggttgcggg cctggccgta tcccaactaa atcgggatct
     2701 tccccatcaa gcactcgagg aggttcttcg ttttccgact tgcgttgctc acgacgggct
     2761 tccttatcct gttgtttcat tcttttgacc atctcacgat ttcgtttggc ctgggttact
     2821 tttgtcggtc gtcccattta tcttcctcct ttcgaatagg gtatcatggc tccggcttca
     2881 ttttttgatg agctggaagg ctttcctagg ctctgcccac ttgccagact atcttgtttg
     2941 gggcaacggt cttcacgggt aaccatttgc cgactttctc gtccggctaa gcagcatacc
     3001 tgcgttacgt ggactccctg cgtgccttgg acggctttcc tgaggtcgag gaacgggaca
     3061 ctttttgagg caccacatca aggcgagtcg cgctatcctg gtcgaaccca ggactcgaca
     3121 tcatcagaac ttgtcggcga acctcatcga tcggcatggg aactttttct ttagaaggat
     3181 cagcgaggcc aaggagatcc caacgaattt tcggttcagg tttgaatgtt tttctatttc
     3241 taaccttacc accccacttc atcgtcagct tcataagaag cctccttctg tctaaagaac
     3301 catgcgaatc atgacaaaga acacacaccg tcctgtcgaa tacggaaagc gaggagctat
     3361 gaaggccata ctccctcacg aaatgatctt cgaagtcaat ccccccagga atgaaaaggc
     3421 aggaaaggca ggagaaccac catcgcttgc accgtaaatc agggtagcac tctactggta
     3481 gggagtcaaa tacaggaaga gattgtagtc gtgggattaa cccttaacct tcaatcacgt
     3541 cacgtcttcc agagcccttg cctctcaacg accggaatca ctcacggcat atgggcaagg
     3601 gtaacaactc tcatctttat gcagggcggt cggacatgcg tctaaagggt ctaaagggca
     3661 acctcttcac ctttgggttt ccatttccgt gcaagatctg ttgcctcgct gatctgagca
     3721 gcggtcatct cctgcgagag aagatctaaa gcttggatcg acggatccat gccctgctgt
     3781 gcagccagat atgcccacat ataggcttgc accaggtcct tccggacccc ttctccctta
     3841 tagtacatga ggccaacatg attttgcgca ccaccattgc cctgttcggc agcccgcaga
     3901 aaccaacgaa aggcttcttg ataatcctgc ggaaccccca aaccctgacg gtacatcgcc
     3961 ccgagattgt tttgagccct agcattcccg agatcggctg cccggcgata ccacaatcga
     4021 gcctgctcaa aatcctgtgg cacgccctgc ccataccggt acatatagcc caacatattc
     4081 tgcgcctcgg tatttccctc ttccgctaag ggtcgccatt cagacagcgc ggcgggaaaa
     4141 ttctcctgca gatatgcctc ctctccttcc tcataaccgg cccacgctcc tcccacccaa
     4201 cagagcagga gaatcatgct tagtttcagg atgttagccg gaaggatcat aatgtcatga
     4261 ctccctttgc cgcacgacag ggtcaactca cgatcgggta aagaccaacc ttcacaggcg
     4321 ctcatatatt cattgtactc tatgcctgtt gcttggcttc aagttccgcc catcgggtat
     4381 acaagcgttc cacttcctgt tcagcggact tgagattttc ataggcttcc tgaagcttgt
     4441 gatgattcgc cgcaattttg ggattttctg tttcagtacg gcacaattcg agtaccgcct
     4501 cggcatcaag gacccgtttt tctatcatgt catattcctg ctggtcacga aacgacagtt
     4561 tttttgcctt tggccgttca gccgtggcac ggacactctt tgattgagtg ccagtctgat
     4621 ctttgcctga tcgttgccgg cgaagccagg attcccattg tccataatcc gcaaattgcc
     4681 cgtatcctcc ctgcccgtct aatcctacaa aatccgtgca cacttcctcc atcatgtacc
     4741 gatcgtgggt gaccagaatc aaggctccgg gaaattcctt taaactttct tccaacactt
     4801 ctctcgtcgg aatatccagg tcgttcgtcg gctcatccac aatcaacaca tcggcggact
     4861 gcaacattaa ccgggcaatg accgcccggg cctgttcgcc accggaaagc aactcaatag
     4921 ggagatccaa ttgttcaggc tgaaactgaa accgtttggc ccatcccgcc agatgcacgg
     4981 tctggccccg gtacatgacg gtatgccctg aatccgacaa cgtccgccgc agggtcatct
     5041 ttggatcgag ttgatcacgg ttttgatcaa aatatacgac ttgcaattta tccgcatacg
     5101 tcacacttcc ctgatcaggc tccaattcct tagcaagtaa tttcaacagg gtggacttcc
     5161 ccgagccatt atgacctaag accccagtgg cggttcccgg tgagatgacc cagtccaatt
     5221 tttggataat tgttcgcggg ccaaacgtct ttcggagatg atggacaacc attaactgtt
     5281 ttgtgcgccg acccgagccg acaaaatcga tggaggtttc ctcttgtcgc aagcgcacct
     5341 gggtctcagc taattcagcc tgcaacgcat gggcagaatc tatgcgagac tttgctttcg
     5401 tggatcgagc cctgggacct cgacgaagcc actcttcttc cctacgaact tttccggcca
     5461 acgcctgagc ctgttgagtt tggctattca gaaattcttc ccgcttaacc agaaactcct
     5521 cataacctcc cggaaccaga aaaatcccat ccggatatct ccgatttatt tctgcaatct
     5581 tatttgtggc atgcccaaga aaccaacgat catggctcac catcacccat ggccatggag
     5641 cctgggacat cacctgctcc aaccaacgca ttccttcaag atcgagatgg ttcgtcggct
     5701 catcaaccag catgacatcg ggcgcctgaa cgaacccaca ggcaatggcg agccgttttt
     5761 tccatccgcc tgaaagaggc ctcacgagct gatcgccctg agcaaacccc atgcgtccca
     5821 atgtctcctg cacccgagcc acacaatcat gggcgtgcag gccggatgcc aaggccgctc
     5881 gttcaatttc ctcggcgacc gtagactccg aatcgaaatc agcctgttgg ggaatatagg
     5941 caatgcgaag atgctgccgt cgagtgactc ttccctcatc cggctgttca agcccggcaa
     6001 aaattcgaag gagggtactt ttccccgatc cattcggccc gataatcccg atccggtctt
     6061 tttctgaaac gcctagggtc agttcctgga agagacgctt ggtcccatag gctttggtaa
     6121 gcttctgtcc ataaatcagc gctgacatac gtatcacact ccttaatgtt ccgaccttca
     6181 taaactagga cataaatatt gtcccttccc cacttgggga acgaatccat tcctgccaga
     6241 tcggactcac gcaagaaagg accctactca acatacctgt ctccagaggc agaaaccaat
     6301 ccgcaggatc tgtttgctaa aggacgacct cacacattct tctgttgaaa tgggatgaac
     6361 accgggcgtc gttggtcggt gccaacctcg aacatcatta tcctaaccgc agtccatcag
     6421 cactctctgg cctcttcacc attctcatac ttcccattcc tggctatgaa cacccaaatg
     6481 gacgtttcag tcgttggaga attttggccc aaagcgtctg atacgtttca cgtccctgta
     6541 agagttcaca atagcgtcca agcacccgtt gataggcatc gggaaaggtc cggcccgccc
     6601 accaatggca agatcgaggt aacccataaa tgatggctcc taatcgagaa gccactcgaa
     6661 attccagccc aaattcagct tccgcattct tttcatattc ctccaaccga tgtcttggat
     6721 cccggatggt ggaggccaca ctggctgccg ccaattgtcc ggatcggaca gcataataaa
     6781 taccttctcc cagtaacgga tcaaccaaat gaccggcatc accaaccaac acggcccgat
     6841 gccgcaccaa gcttccggtc caccgctgtc cagggcgata cgccggatga tgggcaattg
     6901 ggagggggtg tcctagcggg gaaggaatcg aaagcccggc taaagcggat tcatgcctga
     6961 caaaatcctc aaaactgcgt ttaggacgat ttctgccacg aataaattcg cccacaccca
     7021 ccgataatcc tttttccttc ggaaagaccc agccgtatcc ttttttcgct gcgcgtagac
     7081 tgattagtac ggtttgtgag cgctgaaacg tatgaggtgt gtctccatga tattcacttt
     7141 ccaaggcagg aatatttccg acctctcgcc ctggaaaaca ttgttgagcc accacactca
     7201 tcgctccatc ggctccgatg acaacccggc cgcgataccg tcctttccct gtggccactt
     7261 cgaccccatc ctctaattcc tgaatggcga cgacggactc ctcttcccgg atctctgctc
     7321 cagcctgccg ggctttctcg accaaccatt gatcgaaggt ctggcgcatg accatgtagg
     7381 ccacgggacg cggagattcg acaataaagg aattcgttcc cccgtaaata aattgcacac
     7441 gatacaccgt cccctcaaca atctctttga aatcggccgg gagaattcgt tcaattctgg
     7501 ctgaaagccc tccgccacag attttgtacc ggggatgaac ctgcttatca aacgccacca
     7561 cggacattcc caatcggctc aactcataag cggtcgaagc ccccgcaggc cccatgccca
     7621 cgacaatcac atcgtactca aaggaatgaa gaggaggagc cacaggtcag agatgaaccg
     7681 tcgtgaaaat ggacttacga gataaacctg aacaggttag agaggaggaa gctatccggt
     7741 tacaaaaatt gtgtcctctt tcgaaaatct tttactactg tactggaatt ctccctccgt
     7801 tgactacccc ttcctcacac tcggtgacag acccaaaggc caacagtgga gcatgatagc
     7861 ttcggcatgg agaatgcttg accacatcct cctcacgagc tggaagtggg caggtaagaa
     7921 caggtggctg acccgtcaga gaagcccggg ggacttctcg ccaaaacaga caactgggaa
     7981 aggaaaaagg agggagagat aggagagcta gagctcagaa aatgtcgaaa attccgaatc
     8041 cgttgtgtac atgggaatgt gcttctccat atgcatttcg gataataggg tcttcaccgg
     8101 atcctgagga ttcacaataa tcatgcggcc tttaagtttc aggaacttct tagcaaggac
     8161 caccaaagcc cctaacccca cactatccaa aaaggtcacc ccttgaaaat ccaggataag
     8221 cccttgtgaa ttcgagtctg tatatttttc tgcagtctcc ttaaaggcat ttttagaaaa
     8281 aatatccaac tttccggaaa gactcacgac cggcaagcca ttcatgtttc gttctttaat
     8341 aatcacggct tggcattgct ctttcttttt ctatgacccg ctcgcaggta gattacccca
     8401 tgttttttgg ctttggtggg tctatcggat catttagagg gaatcttgag gattatggcc
     8461 tgattcatga cctttcaccg gcgtaacttg ctatcttgag cctcttccca ggatgagaaa
     8521 tggactctca aaaatcagtg gaagggtcag caggaagaaa aaagaatagg gttgaggaat
     8581 atctgccagg gaagatgaac cagatttcct tgtgcctgaa ggcccgtcat agaacccatg
     8641 gggacctcac aaagacaggc aagccccatg caggaagcca aacaaacgga gcggatttat
     8701 tctcaatttg aatgccctct taggatcggc ctaacgtaac cgagagcagc tcctgagaat
     8761 ctccacgctg aaccctgagt tcgacaactt ggcccggttc cgttcgctca atcagataat
     8821 ttttcaattg tgccggtgtg gtaatcggca catcattaat ggccaccaac acatcgcctt
     8881 tttgaaatcc tgcatggcgt gagggaccgg tcgctttggc cacgaccagc gcccaacgac
     8941 ttcccatcct caatgtctgg agctgaatcc ccagcaccga aaaccccggc accctgtcag
     9001 ccaagacggc ggtcaccacc cgttgaacca acggtcccgg gagaacaaaa gccaatcggg
     9061 cacaccgtcg atcgtcatgc tgcgtttccg tttgaatgat ggcatgcatg agccctacca
     9121 gttgtccatg ttgattcaac aaccccccgc cagaattgcc gctacacacg gacatatcca
     9181 cctgcattaa tcgactgttc accgtcggca aaaacgtatt gggatttcca gtcgtgccaa
     9241 agttaatcga cgggccccaa cccagtgggt acccgaccgt aaagacctgg tcgccatatg
     9301 ttacatcttc atcggcaaac gtgacctgcg cttgtggcca atccgattcg gcaccctgga
     9361 gttgatacac ggccacatcc aggtaagcat tggctccctg gcgagcggcc ggcaattcaa
     9421 aaaggtcatc cgtcaccaca tgaatggtgt ccggaattat gacttttcct ccttcggttc
     9481 gctctaccgc atgccgtgcc gtcacaatca ctccccggcc aatatggatt cccgatccgc
     9541 gaatactgac cgggagccca tagtcattgt ctggaatctc aagttgatca tcctgcagta
     9601 tcccgactgt cgccaccttg gcctgttcaa tggcctggga aatcgacatg gccgaagact
     9661 ctcccaaccc cgcgaacaag ccaagcccta caaaaaaaac gagtccaatg agaatcgacc
     9721 tgtcatcact catccttacc cccagcgttt cagaatggcc cgcatagcgc gtcaccacac
     9781 tcctccttcg aaccgactca ctctctcacg gttattcaac gagaacaccg atagctaaga
     9841 cctatctctg aacacctcat ccacaaaatt aacgtgtcct tctctgccgg aaagggacca
     9901 ctggtttgtg tcgacaactt gcccgccata cctgcggctc acgcaggttg gctccaatcc
     9961 ttcaactcac cgaacaaggc tttcatatgc tgataagaat aatgggcatt tcttccactc
    10021 ggatttggca acacacagac aggagcaccc ccaagggcaa gagattgcag gccaacctga
    10081 atagggtttt caatcaaccg ttggacagcg gtggtcgcct tcttggacga gagaagcatg
    10141 cgagccatac tgactcctaa caaggccacg agtcgaggtt gatatgtcgt gatttttgtc
    10201 actaatgcct gatgaccttc cagaaaatcc cgcttttcca aatcctgaac cccggcggtc
    10261 ggccgggcaa tcagattggt aagtccatat ccgaattcgg gcaattgcca atcatcctga
    10321 taggtcatgg gaagtggaat cagcttggcg tcaaacaata atttccaaaa ccggttggaa
    10381 gggccggcaa aatgatgccc gacctcagca gagcgcagac ccgggttaat tccaacaaaa
    10441 agaatagata acccggggac cagataatcg ggaagacctg aatgcggatg acttgtggcc
    10501 atgcgacctc gatcgttggt ttcacccaca cgcatctcca tatcttgaat atagaaagtc
    10561 cggaattttg cggtgtggcc tctagcacgg ctacgacaat cgatcggaga atacgagaaa
    10621 attgtggaga gcaaaacgga aatgctgggg aaaggatttc atctaaaccg gccaccgggc
    10681 aaaacccgaa tcgtgattta cttggaggcc agtttcgtct gaatttccga aaaggttttc
    10741 tcagcctcag ggagaagatc atcccggttc cccgccctgg caagctcgag cgcctttttc
    10801 gctaaatcaa aagcctcttg atccaatccc cgctgggcac acgaacgcgc gacgaccatg
    10861 cgggcattaa tggattgagg atcctgctga atcacccgcc gcagcatcgc ttcgcctttt
    10921 tcggaatctc ctccaaataa cccgggaact tcgatgagga tcgtcccttt agaagaaagg
    10981 gcatccatat gattaggatt gagcaacagg gtcttgtcca gagcttccat catccgtcca
    11041 tattcaaaca ccgagaaaag aacttctccg ttggcgcgca tacgctcgcc aatattacag
    11101 aacagggcaa aatgcgcatc ggctgaacgc tcgtccaacc ccacagccgt ttcagccaac
    11161 tttcgccccc gctcaaaatg agcaacccga accgtttcct cctttgcccg tctcccttca
    11221 tggcattcat ccatcgcctg ttttgtcaga acctgagaag attcttcagc ccagactccg
    11281 gggatcaccc accaggaaag cagcaaagtg gccaataaaa tgtaaagcat tcgatgagta
    11341 acggtcatct tgtccaaatc ctttgtgcgt tactctgagg aactccagga aatgtaaggg
    11401 gatacagaaa taaaaaaata ttttatatca agttcaacgc tcatgccaac gcctaggtgg
    11461 agaattttta ccttgtttta cccagggaag agacattccc catttgggtg gaccaacgag
    11521 aaagagtcta tgcccacagc tctacaatga atgggcggtt gtcagaacgt gcccaaagtg
    11581 gggttccgcg tcgattgaag ccatcttgca gcaccaacac ccgcagcata gccactggca
    11641 aaacaagcag tcagaagata gccaccggtt ggggcttccc aatccagcat ctctcccgca
    11701 caaaatactc ccggcaaagt acgaatcatc agtcgctggt ccaaggcttc gaaggccact
    11761 ccgcccgccg tgctaatcgc ctcctccagg ggacggggcg cgacgagacg caccggcagc
    11821 gctttgatgg ccgccccgag tagcacgggg tcagcaaagt cttgctttga cacacattcc
    11881 ctcagaaggc cggcttttac ccctgaaata ccaacccggc gctgtaggtg actagccatt
    11941 gaacgtgaac cacgcgggcg tgataaatct gaaatgagcc gcgacacatc acggtcgggc
    12001 gccagatcca atcgaagaag cgccgtgcct gttttcacaa tctcgtcgcg catccatgcc
    12061 gaaatcgtat agatcacgcc tccctcaaga ccggtctccg tcagaacgaa ttcgccctgc
    12121 tgtcggaact caacgccctc ttgagtggtt cccgcaacgg caaccggctt cacgggatga
    12181 ccggcatagc ggccacgaag gtgcggactc caaacaacat caaacccaca attcgcaggc
    12241 tttagtggtt caattgcaac acctcgcccg gcgagcaacg gcacccacgc cccatctgag
    12301 ccgaacctgg accaactgcc accacctaac gccaacacca ccgcatcagc agaaacgaaa
    12361 cgatctcctt ggggcgtggc aaacctgagg gatccggtct cggtccaacc accccagcga
    12421 tgccgcacgt gaatcgccat accgtgctgg cgcaaccggc gcagccacgc gcgcaaaaga
    12481 ggtgcagact tgaggtccgt gggaaacacc cggccggatg ttccgataaa cgtctcaaca
    12541 cccagctcat gagcccactc gcgtagcgca gccgggccaa atccggcgag caacgattgg
    12601 aactgtccct gccgtgcgcc ataccgtgca agaaatctct ctaatggttc agaatgcgtc
    12661 aggttgagcc cacccttccc cgccagaaga aattttcgac caacggaagc catggcgtca
    12721 tacagatcca cgcatgcgcc ctcggcaatc gccgcctcag cagccatcag accggccggc
    12781 cccccgccaa tcaccgcaat gttcatggtt ccctgcttta acatcatgac aaaccgggat
    12841 ctaggtgaca gtcaaaaggc gttctcaggt gattattgtc gattgccgtc tctgagcgtg
    12901 catttcggag tctaacccgg tcccctcaga agaatttccc cacaccaatg ctcttcatca
    12961 caaatgtgaa ttgcccgaag agaacggttt taattaagat acactgagcg catacatcag
    13021 gatcacttct ccccctagta gacttcataa gacaggaggc cggaattcat ggagcaggaa
    13081 gaaattcgac aattgtgggc cgacggtgag gattggatca tcaagcgcca acacaatcaa
    13141 tacttccatc gtccagatgg aaaatatgga gactggaagc ccggactccc tcccggcgtg
    13201 gtaaagccgg acgtcgacac cctcttcgat aattaaacga tgggctctcc ctcatcatca
    13261 gatccgaaat tcccatatct caaaaaaatt cttctcaact gatcaatcat tccagagaga
    13321 gtctcccgcc acgcctatgc gaatttccgg acgtatcatt ggtctgctga aggaatacat
    13381 gcatgacttg gtcgaacaag ccaaacagga ggaagccgcg catgccagct tcggatttgc
    13441 ttcccaaccc tatcgggctg acgaggcgat ctcagatctc ctggctattt tggatgaccg
    13501 tatcgaatca gaaggggtcc aggttggcct tcccatcgag tttctgcatc agatgtggac
    13561 actgtgcaac caagcaacca cgcacatttc ggaaaccgtc tggttgagaa aaaatctgga
    13621 ccagggcacc gggtcgaaag cggaagtccg gcacctcacc taccaagcgc tcattgcatt
    13681 cctggagtct cagcccgaag atgacgagtc gggagacgcg actccaaact gaatggctaa
    13741 ccagccatcc catcccctga catggcgttg acgccatccg cacccttcaa actgtcgaac
    13801 gatctcagat tcatcttcct ccagcagccc ggataccacc aactgcgttt gcggaccccg
    13861 catcctggca aattgatcga taacgtgaag aatcgttctg cgatccaggt tggccagaat
    13921 gacatcaaat gattgggccg gaagcgtggc caactgggca gaggaaagtg tcaatgcttc
    13981 ttgaatgttg ttcaccacgg cgtattccct ggcacagtcg agcgccgtcg tatccacatc
    14041 aattcccagc gcactggtcg ctcccaggcg taatgccgcc atcgcaagaa tcccactccc
    14101 cgtccccaca tccagcaccc gcatgcctgg aacccacgtg accccttcca accactcgag
    14161 aatcatttgg gtggtggcat gatgaccggt cccgaacgcc tgcttgggat cgataatcaa
    14221 ttccactcca tcctcgggca tctccatagt ggcccaactc gggcgaatgc caattcgacg
    14281 gccaatgcga atgggctgga cagacgcggc ccatgtggca ttccaatcct gggccttcac
    14341 cggatgaaac cggagagccc cttccggaag aaccacaccc aatacgctga tcgccgaccg
    14401 gacctgctga agaatctctg ctccattcct actccaatac aacacggtgg atccctccgc
    14461 gtcccatgct ccaagaaatt cctggcaatc gagaattccc gctaactcag cagcatccac
    14521 ggaaatggga atggtgattt caaaggcagc agcatccata agaactattc cctcgttaac
    14581 aagtttctta aaagtaagcg actcctgcca cccaatcctc agcggaaagt cacagaaggt
    14641 tctcttcaaa taggcgccag ctggggggac ccaggggctc gttgagggct ctgtcccatg
    14701 agaacgcgcc tgactgtatg gtttaacggg cacttcacaa aattccacgt tattgacggg
    14761 atcacgcaga gcggctaatg tccgcagtcc ctatgaaaca taccaccact gatagacgag
    14821 aacaggcgat tcttcgcctc attcaacgca ccgagcggat gcaggccaag ggcaccatcg
    14881 ccagccggca attcagccga tggagaatgg gaacattcct agcgggagcc gccatcacga
    14941 tcggagtgta tcaacaagcc tggtttcata ccggcaatgg tctcctgctc gtcttcctca
    15001 ttggatttct cactatatcc tggtttcacc agcgattgaa atcccggttg catcgactac
    15061 agctctggtt ggaaatcaaa aaagacagcc tggctcgatt gcaacttgat tggccacaca
    15121 tatccgccta cgaacaccgg gcccctgagc gacatccatt tgccatagac ctcgacctca
    15181 cgggtccgca ttccttactg gctttgatcg acaccacctt ttcctccata ggtcaacacc
    15241 gcgtcgccac ctggcttttc cagtccaatc ttcccgaatc agacgggcta gcatggacaa
    15301 ccagacagac attggtcaaa gaactcactc cactctcccg tttgagagat cgctgccttt
    15361 tagccaaccg gctcatcagc tccgatcggc tgaatggaac ccgcataatt tccttgctcg
    15421 aagcacccgt ctccatccgg catttgccgc tgattatctt cgcagcatgt gggctgacga
    15481 gtctcaccat cgtcctaggc ctctggacgc tcctcatggg ggccccgaat ttttggctgc
    15541 ttcccctggt cttttatatc ggaacctatt ttctcctatc gggatccatc cacccgcttt
    15601 tcggccgggt gctcgccctc catgaagaac tcgaaaaact tgtcagcgtg attacggaac
    15661 ttgaacgacg aacctttctc catcaaccaa caatggtaca agtctgccaa tccattctaa
    15721 ctcaacagaa tcgtccgacc cagagcctca aacagctcag ccggatctgc caggggctca
    15781 gcgtcaaagc ccacccgcta gtgcatttag gcctcaatgc cctgataccc tgggacctgt
    15841 ggtttgcggg aaaactcaat cgcctcatct cgcgccttcg cacgacactt cctgcctggc
    15901 tggataccat tgggacagtg gatgccgccg gtgcgctcgc gcgttatgcc tatctgaatc
    15961 ccgattacct ctggccacaa ctcgaaacca ccaatacaac tccggacaca caaggactga
    16021 ccgcaggcag cctcggccac ccgcttattg ctcgtgcgca tcgcgttacc aacgatctgg
    16081 agttacgagg ggaaggccat ctccttctgg tgaccggctc aaacatgtcc ggaaaaagca
    16141 cctttctacg aacggtcggc atcaatttgt gtttggctca ggccggtggt ccggtgtgcg
    16201 ccaaaatttt ttcctggacc tggatgcgtc tctattgttg catacgagtc acggatgccc
    16261 tggatgaagg cttgtcgtat ttttacgccg aggtcaaacg cctgaaagtt attctggagg
    16321 cggtggaaag caccaatcct cacccggtcc tctttctcat tgatgaaatc tatcggggca
    16381 ccaataaccg cgaacggctg gccggaagcg aagcctttgt gcaggccctg caacaaagcc
    16441 gtggactggg tatgatttca acccatgatt tggaactcac aggattggcc agtggaaacg
    16501 ggcatatccg gaatgcccac tttcaggaaa ccgttgaagc aggcacgttg catttcgatt
    16561 atcgactgcg accgggcccc tgcccgacca ccaatgcact ccggatcatg gcccaggaag
    16621 gcctaccagt cccacacggt ttttactcag cgggaggaac aagacccgat cggctttagg
    16681 gaacaataag gcgaacttcg ctttgaccct gatcggtctt agagccggtg gatgtgccgc
    16741 aatgggcaca gaggtattcg taaagacttc cggtagggag cactaaaaga agacgttcac
    16801 gggctggcgt cgcagtctga cattttggac aaaacagcaa ggacgctcga agcgattcga
    16861 attgcttggg tgtgcctgga ggagccggtg gacgagcgcc agccatccgt ggcgttggcc
    16921 ggggacccgt aggaaatggt ggtctaggca tacgcagatc ttagcgaagg gccttagtcc
    16981 ggtcaagatt ccaatggtag agggaacagt ctcaattcat gggacagaca atgactgctg
    17041 tgaagtagtc tagttaaatg aacacgtcaa aaaaagcgtt aatcgccaaa ggaggtgggt
    17101 ttgggggagt cagggaagga aacaggatcc ggccgacttt aaacggggtg ccgttgcatt
    17161 cgtcatccac caaggctgct ccgtagccga ggcagccaag aatatagggg tgaacgcggg
    17221 cttgtttcgt tggtggaaaa ccgggatgag gccgcagggg ggcctggcgc ctttccggac
    17281 aaaggccaca aaacgccgga gcaagcggaa ccccatcgcc tacaggagga gaatcggcag
    17341 ctccggatgg aacgggattt ataaaaaaag ccatgacctt cttcgcgagc gaaccgcaat
    17401 gatgtatgcg tttatacacc gcgaacggtg gcagtatccg ctgaccgtgt tctggcgggt
    17461 cttgcaagtg aaagaattct tgcaagtgaa agaatcaatg caacgccgac gtcctaaatg
    17521 agtttttcaa tctaagatgc cttgtcttcc ttagaggatc gaagaattcg ctgagtcatt
    17581 aacaagagtg atgggatcga aaaacagctc tggggacagt tagggcgaga aatccgcaga
    17641 tggaataagg gacacaaccg gaatttaaga aacgatgagg ccaacgtcgc tttgagcttg
    17701 atcggtgcta gaaccggcat gggtcccgca atgtgcgcaa aaatagttgt aaccgctcaa
    17761 tcatctgttt tggcctatat aaggtgaaag gaatgccaga ggattctgtt ggatatgtac
    17821 tgattcagaa ataacctgat ttcgaaatac ccggcgcaca tcgtctcaag atgtccttct
    17881 tttgcctaag cagcctggca tcttactgac ttctcaatgg aaagctgatc aatagtcctc
    17941 caatgacgtc accgatttta aaatcggatt tcgggctgtt gggatgggaa ttgtggcatt
    18001 ggacgcacac cgtagacaca gccacatcgg cataaaccgc ttcgaacacc tgtccctcgc
    18061 cggccccgat ggtactggaa tacggtctat tgggatgcat ttggacctcc tttagagcca
    18121 tccgctcaaa ttcattggct ggagcattgt ccttattgat cggccactga ctgatcaggc
    18181 ggtagcggac attcggactg gtcaattcaa ccatctcgct agtctcctgc agaagttgca
    18241 caggcaaagg caatgaatgt gtggcctgcc agttttccga ggcatcgaca atgcgctctt
    18301 tctcgagtcg gttgacgata tggatggtat aaaagtgccg gtgggccatg atgactgcac
    18361 gtagatagtc ggcaacggtt ctgggaggaa tacccacatg gatgccggaa ttcttgtcga
    18421 tggcgaagac gttcgggaca gccaccagta cgatggttac aataatagtg gcccaggtca
    18481 atgatttgct ggtcatgatg tcctctcttt tttttcgatg ggaatgagcc ccacaggtaa
    18541 cgggatagag tcaaatccat gtaaaaccaa gagcttttgg atgcgaggcg tcatcagaaa
    18601 atcagaaaac tgttctgcgg tagaacgtaa ggagtctcga caggtccaca cgactgcttg
    18661 cccgaataag atcggcacat acgcccctat cgggttttca tcgctgatgc gtatatgtcc
    18721 attagagatc gaatctacgc gaaagaccag ccccacatcg gccttgccca tgcgaatcaa
    18781 attcatgatg tcttcgctat gtgccccgta gagaatgtga ggacggcttt tgtaggtcgg
    18841 atagaatttt gtcagcaccc gggcggtgac atctcccaac gaggacgtct ggggatcccc
    18901 aagggcaatg cgtgtggact ggttggacag cgcatcatgc aaggaaacaa acagagccgg
    18961 ggagttcgac gacatcacaa gaacaagtga cgtctgggcg tagatccttg ggcgtccgtt
    19021 gagagtcagt cctttcttgt acaggtactc aacttcttca acccctgccg acagaaacac
    19081 atcgatcgga gcgccctttt cgatttgctg aagaagcgtt ttcgatggcg tgtagacgac
    19141 attgactgcc acgccatatt ctcgctcaaa cagcggcaga atatcgttaa aagccggtct
    19201 taaactggga ggtgctccaa ctgtcaaagg ttcagcctgg accggctccc aggtgccaac
    19261 catggccgcc agtccagcga tagcggcgat cagtatcttc aaccccgcaa tgaccactct
    19321 atacctccgc aggtccgctg aatccgcgaa ctatgcccat atgctagcac gcatgacatt
    19381 gcggaaaatt cagcaagaga catgtctggt aaaaattccc gggggggaac acgaacagaa
    19441 ggcgttcttg agccgatgta gcagtctcac acgtccaaaa cgacaacggc aacaacgccc
    19501 gggggtattc aattgggttg gaaaggttct gaggaaacgg cggatgagga ccacccatac
    19561 gtgactctaa ccgtgaaccc attggaaatg gaatactcta tattcacgaa tcttagcgaa
    19621 gggttatagt ccggtcaaga ttgcttcgca gcatgaaaaa tccccatatt ggctgaccca
    19681 ctatttcgag ccacactcct cagatttcac accaacccac accagttccc tgtcgctgaa
    19741 tccgatcacg gtggatccac tccccgccgc ttggcaattt cttgcgcgat aataacctgc
    19801 cagccttgat taaataaatc tcgcgtggcc tgtatcgtgg gaacgggaag gggccccgct
    19861 tcatgggcca accggagttg ttcaaaatta tccatcatga gcgtcaactg cttagccgcg
    19921 aattcattca cgtgatcact ctgtgcccgc agtcgcagtg agcgaagctc gaccagatag
    19981 tcctgataaa agacctgatc ccacgaatag tgagcctgcg attccccagc atgggtttcg
    20041 agtctcgtta aaaaagcatc catccttttt tggagcgagg ttgccgtccg atccatttcc
    20101 ggatcataag aagaaattaa cgtgacgtgg caaccaacca gccccaattg caaacacgcc
    20161 aaaacacaga gaacccatcg agcagcaaat gcaaagggaa atttcatgat gtcagatacc
    20221 attgggaatt cacctttttt gttgaaattc caggtgggaa tcgcccgcgg gacaggattt
    20281 ttttgtgcgg gcccctttca gaataacgac cttatcggga catgaaacag ttgaacctga
    20341 atctattgta ctcacatgtg tccatttgtc cagcaggttc cgatgaccat accaaccgtc
    20401 actcaactcc agccaatgat cccccgggat tattctgttc gaggccaccg caaatgctac
    20461 acatgatcct gccagtgatt gagatgctct tcatggcggc aaggggctcc atacccattc
    20521 gcactggcga ctctattcta ggcggatatt atccacgtac agggaaaatt cttccggtgg
    20581 gcttatggca aagagtcgaa cattcttgat cgcgccaaga tccaattccc gtccaagcgg
    20641 ggcatggcga atgtcatcca ggagaatgtg aagatggttg aggcctggcg aaattatgac
    20701 ggcccgatta aaccgatccg catatccgtg attatgagga gcatcatcaa ttcgaacggt
    20761 cagcgatcgc gcttcgggca attcggaata cacctcaacg cggaaatgcg aaaaccctcg
    20821 ccaatccgga tagggctcct caaggcgaat acccggatag tgtgccgggg aacacaccat
    20881 gtgccccacg gtatcgaccc gtgacttctc ccagcctgcg ggcggaggaa caacctgaac
    20941 ggcacagtcc ctccctttaa caaacatcat ttcccatgcg gaagaaaact gaacaagcga
    21001 agggaatcgg gtagcccggt cccaataggc ataggtccag acaaccaccg gactcagggc
    21061 tatcaccacg agcaacccga ctccggcatg aaccaggtgt ttccttgggg ctaaccgcca
    21121 tgtggcaaca cgtcccgtaa atcttgaatc gtatgtgaca tacattccca gcgcagccac
    21181 agcccccgaa acatcccgac aaaaatccga ccattccgca aaacgactgg aactgaacga
    21241 ttgcacccct tcagtcaatg cacccagcgt cagggcccca atacaagccg tgccatattg
    21301 tctacccaca gaccaacctc ggggctcacc aagctgccgt gccagatata agaggaggat
    21361 ggccacgcca ccaaataatg gaaaatgaca aaaatcgtag aaggctttca gaagaatggt
    21421 atgagagggc acgggaatcc aaaggagggc gatagaacca acgagggcca gcgtcagacc
    21481 agggaccatg aaccgggatc gcatggatcg ccgattggtt ttctctcctg gctggtctgt
    21541 cacaacacgc tcgtcaagcc agactcacga cggagacggt gtcggcagac ggcacaccgt
    21601 tgcgaaaaaa aatcggtttg gatgtgtgag cggagatccg ttcgcccggc aaaatgatgc
    21661 cgatcagatt taacggatcg gcggccgaca gacgaatctc ttgcgccccg gcttgaatat
    21721 cacggcgtac cgcccgcaac gattccaccg cctccggcaa tgcgaactgt tcccccgtaa
    21781 acccattcac aaaccggcca ccccgcatct caccccgcca ttccagcttt cggaattgta
    21841 ccaacagttc ccgccaggtc actggcagtg attcccgttt gagcagatcc cgaaagacca
    21901 ccccataccg atgcgccaat tgcctggccc actgctcatg tgggtgaaga cgaatctgcg
    21961 attcctcatc atgtgcatta ggatcaccct ggaacagatc ccaacgcccg acgctgtgcc
    22021 ccggccgtcg cgcgcgatcc ttgccctcgg ctcgccggcg tcgaggatca agcagggccc
    22081 gcatgggatc aaacccgtca gcggtcacca accctgtggc caccagttcc cacaaggcct
    22141 gctccacttc cgcgggcaga tgtcccgttc gaccggtcag atccgcaaaa aaacatgcac
    22201 cacgacgatc cagacaatcc caaacggctt gtgccacgcc actcagatgc aacccccctc
    22261 caagctgccc tgcagttgtt ccaggtttgg ccacacccaa gatccaactc acatcctgcc
    22321 ggcgcaaaaa actaatgggc gccagcctgg ttggagtcag cggccgaaat gctggcacag
    22381 ccgcaccggc atctaagaca aagttgccta ttccctgtcc tgtccctacc acaggtaaga
    22441 gggtgccctt gctttccgga ggggtcaggc gtccccaggc cacttccccg cgcagacaca
    22501 aatgatccag ccattcaggt ttgtagtgag ccaggcgagc tgtgaagagc gaaggttccc
    22561 acgccgaagc cggggcttca aatcctgcca attggtgaat cacttcacgt aacccggctt
    22621 ctccgtgcaa ttgtgttcca ggcgcccgat gctgccatcg aaagagaaac cgcataaaat
    22681 ccgatgcggc gaccggctcg atttcccggc gcaaagacgt gaccgtacgc cgatgaatac
    22741 gagccagaag actccggtca caccattctt cttcgactgc atggttacct atcctctgtt
    22801 cttctttcgt cgaagagccg gtcgaagaat caatccggga ggaaggacga aaatgacctc
    22861 gaaggacctg accttctcct tccaattgca agagagcctg ctgcacgagt agcgcaggca
    22921 catttaattt tctggacaat tcatcagcag tcaccggacc gacgtgcggc atccagcctt
    22981 gcacgacatc acgtgcagca gatgctgccg tggccgacgt atctcccaca gagaccgcac
    23041 gtgaatcctc tagaccggcc accacctttg cctccgggaa caagacctga agacgggtca
    23101 aatgttcttt cgtggtccaa ccttcaacaa ctttcgtttc cggaagtccc tcaaccggta
    23161 cccgcaacac caggagacgg ccacacgaca gtaacgcctc acccaactca tgccacaact
    23221 cacccatggc gaatggcaac catcccagaa cctgaagcac gtcgaacagc tcatcggcat
    23281 cacgcaccaa cggccgggct tcttcaacca ccgcctcaat cgctgccgca tccaatgtcc
    23341 cgatgttccc gtcaaaggca ggggacgagg tccggcgaag attcacggca cgcgcgcgtc
    23401 gttcttccag gggcgcatca tccaggaatg catagggatt ggcattcaaa atctcatggg
    23461 caaacaatga aggcacaggt gattccacca cccgcacctg aatctcctga ttctcgatcc
    23521 gctcaagcac ctgacgcagg cccgccagat ccatggcctc cgtcaaacaa tcatgcagcg
    23581 tttcctggac caactgatga tccggaaccg g
//
LOCUS       DATIAL010000294         5302 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_915278, whole genome shotgun sequence.
ACCESSION   DATIAL010000294 DATIAL010000000
VERSION     DATIAL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915278"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VLA60_09240"
     CDS             <1..687
                     /locus_tag="VLA60_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSF09588.1"
                     /translation="CQKLTMACSRLNALFEEEVLSDYVRGPVGPLPTEDIPEAVQRIQ
                     GEVDHVRGITSGLLQFIRLGQIGLRWEGLDVNQLVMGIVTSMERKLKSREVLVRIEDL
                     PDCMGDEVLVTQVFSILIDNAQKYLDPARPGEIIISGKTMHGWSVYSVGDNGIGIAGD
                     HHAGIFEGFSHPAVEAEGRQGVGLAIVRRIIDRHQGNIEVDSIPGQGSTFRVYFPRAI
                     IVEEGEGKEE"
     gene            complement(1053..1520)
                     /locus_tag="VLA60_09245"
     CDS             complement(1053..1520)
                     /locus_tag="VLA60_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF09589.1"
                     /translation="MRPKGSAAKLETRRRLAASLLARGRGIREVARTIGAAPSSVKRW
                     KDALEQGGKNALKSKPHTGRPSRLSPKQRQHLLLMLKRGPQAAKMRSDHWTCRLIGRM
                     IRQYFGVKYHPDHVSRILRGLGWKNKMKISPIKKNRRTTQPRLRRLGQSRRHR"
     gene            complement(1711..2364)
                     /locus_tag="VLA60_09250"
     CDS             complement(1711..2364)
                     /locus_tag="VLA60_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HSF09590.1"
                     /translation="MKLSKHSSPSDSSPFQTWTVPQPDQHSALLLVDLQNDFFPGGAL
                     AVPEANSLIPLINAYIKHFSRQGWPILATRDWHPANHSSFTEQHGPWPVHGVQGSRGA
                     QFHSDLVLPPGMMVISKGTDPKKDSRSGFDGTSLGDRLEDLNVQTLFVLGLPTEHCVK
                     HTVLDGCQRGLRVVLLTDATKGSDLQQDGSPNVLQEMTDAGACLANSRDIGIPASFR"
     gene            complement(2370..3263)
                     /gene="argB"
                     /locus_tag="VLA60_09255"
     CDS             complement(2370..3263)
                     /gene="argB"
                     /locus_tag="VLA60_09255"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HSF09591.1"
                     /translation="MNKLIKKADILIEALPYIRTLAGKTVVIKYGGSAMVQEELKEGF
                     AEDVVLLKYVGLQPIIVHGGGPQINEILDKFGIEAKFLHGVRVTDQPTMEVVEMVLGG
                     KINKEIVSLLNQHGAKAVGLTGKDGRLLTTKPFNPKSLIHTYSGSTDVLHPEDYGLVG
                     QVSHVDTTLLHTLQEANFIPVIAPIGVDAKWQTHNINADLVAGSVAAAVQAEKLLVLS
                     DVKGIRDANNRHVSTLSRKDMERMVKKQVISAGMLPKVHACLTALQGGVRKAHIIDGR
                     VPHAVLLEIFTDKGIGTEILA"
     gene            complement(3348..4751)
                     /gene="hslU"
                     /locus_tag="VLA60_09260"
     CDS             complement(3348..4751)
                     /gene="hslU"
                     /locus_tag="VLA60_09260"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HSF09592.1"
                     /translation="MEQETSPTPKNLDSLTPRQIVEELDRYVIGQRDAKRMVAIALRN
                     RWRRQQLSPELRDEIVPKNIIMIGPTGVGKTEISRRVSKLADAPFIKVEASKFTEVGY
                     VGRDVESLIRDLTDLSINMVKAAYLEKVQKKAEDMAEERVLDMLLPPSPSSPSFENTE
                     ESGGSPTQQKQDQQDSTRSKLRLQLREGKLDNRMVEIEIKERGGLPVGIISNIGGMED
                     LEHNLRDMLGGMMPGKKKNRRMKVSEALKFMAQEEAQKLIDMEEVTKEAITRTEQSGI
                     VFLDEIDKIAGREKHTGPDISREGVQRDLLPIVEGSTVNTKHGPVKTDHILFIAAGAF
                     HVAKPSDLIPELQGRFPIRVELEPLTKDDLIRILTEPKNALVKQYHALLSTEGIALEF
                     TKDGIEEIAATAVQVNDRTENIGARRLFTIVERLLEDVSFEAQDLQEKKVVINAQYVR
                     DHLQSIVKDEDLSRYIL"
     gene            complement(4755..5291)
                     /gene="hslV"
                     /locus_tag="VLA60_09265"
     CDS             complement(4755..5291)
                     /gene="hslV"
                     /locus_tag="VLA60_09265"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HSF09593.1"
                     /translation="MKIRSTTILCVRKGPTVAMGCDGQVTVGSTIMKSNAKKLRKMHQ
                     DKVLTGFAGATADAFTLFEKFDAKLEEYRGNLTRAAVELAKDWRTDRVLRRLEALLAV
                     ADREHSFLITGTGDVVEPEDGILAIGSGGPYALSAARALLAHTELQPSQVVEQSMQIA
                     AGIDIYTNHHIVIEELSS"
BASE COUNT         1155 a         1267 c         1453 g         1427 t
ORIGIN      
        1 tgtcagaaac tcaccatggc atgcagtcgg ctgaatgcat tgtttgagga ggaggtccta
       61 tccgattatg tgcgggggcc agtcggtcct ctccccacag aagatattcc cgaggcagta
      121 cagcgtattc agggtgaggt agatcatgtg cgtggtatca cgtcgggact tcttcagttt
      181 atccggcttg ggcagattgg ccttcggtgg gagggcctgg acgtgaatca acttgtgatg
      241 ggcatcgtga cttccatgga gaggaaactc aagagcaggg aagttcttgt gcggattgag
      301 gatcttccgg attgtatggg tgatgaggtg ttagtgactc aagttttttc gattttgatt
      361 gataatgccc agaaatatct cgatcctgcc cgcccgggag aaattataat ctccggtaaa
      421 acgatgcatg gttggtcggt ctattctgtt ggggataacg gaatcgggat tgcgggagat
      481 catcatgccg gaatttttga ggggttctcc catcctgctg tggaggcgga gggccgtcaa
      541 ggggtgggtt tggcgattgt gcgtcgcatc atagaccggc atcaaggaaa cattgaagtc
      601 gattcaattc cgggtcaggg ctcgaccttt cgggtgtact ttcctcgggc gatcattgtc
      661 gaggagggag aagggaaaga agagtgaatc gaataaaatg aagacgtatt tttcccagca
      721 atcagtcagt caactttggc agaggccctg gcctgcttca aatagtttcc tgaagttgtt
      781 gttagtcggg gtgtgcgtcc gggaccttct ttcagtcctc cgcgaatcaa cacattttgc
      841 gcgggtttgc atggaacatt ccttggggcc tgatcccgta atagacagaa aggtcctctg
      901 acctgaacgt ggtggtgaat ggcaggggag ggacgatctc taaggaaaat tgaaaaggta
      961 tcgagaaatg aaggaagaaa gcaatattgg ctaggagtcg aaactgatct ctccgtcatg
     1021 gcggattgaa gtgacctcca tcgaaaaatt tcttatcgat ggcggcggga ttgccctaaa
     1081 cgccgaaggc gtggttgtgt cgtcctcctg ttttttttaa tcgggctgat cttcatttta
     1141 tttttccaac ccaatcctcg caaaatccgg ctcacatggt cgggatgata ctttaccccg
     1201 aaatattggc gaatcattcg gccaatcagg cgacaggtcc aatgatcact ccgcatcttt
     1261 gcggcttgtg gtcctcgttt gagcataaga agaaggtgtt ggcgttgttt gggcgaaagg
     1321 cgggatggac gaccggtatg gggtttggac tttaaggcat ttttcccccc ttgttcgagt
     1381 gcatccttcc atcgtttgac ggacgacggc gctgccccga tcgttcgtgc gacctcgcga
     1441 atgcctcgac ctctggccaa caaactggcc gccaatcggc gtctcgtttc tagttttgct
     1501 gcactaccct ttggtctcat gaatccactt ctcggatttc tccctgcaac tcttgaggat
     1561 aaaggtgcat catccgttaa aaaatcaaat gaaggactcc tgggagaaaa tgacgaaaag
     1621 cgcggatgtg atgatcgagc aattttccca agtcgaagca tggggaaaat cgccagccgt
     1681 atggcactta aggagtgggg cagaaactgt ttaccggaaa gaggcgggga ttccgatatc
     1741 gcgactattc gcgagacagg cgcctgcatc ggtcatttct tggagcacat tcggggagcc
     1801 atcctgttgg aggtcgctcc cttttgtggc atcagtgagc aagacgactc gaagtccgcg
     1861 ttgacagcca tcgagaaccg tgtgcttgac gcaatgttcg gtcggtagtc ccaggacaaa
     1921 caatgtttgg acgttgagat cttccagccg gtcgcccaga ctcgttccat caaagccgga
     1981 tctcgagtct ttcttcggat cggttccttt agaaatgacc atcatcccgg gaggaaggac
     2041 caagtcggaa tgaaattgag ctccgcgtga tccttggacc ccatggaccg gccaaggccc
     2101 gtgttgttcg gtgaaggagc tgtgattagc cgggtgccaa tctctggttg ccagtatggg
     2161 ccagccttga cggctgaagt gcttgatgta ggcgttgata agaggaataa ggctgtttgc
     2221 ctcgggaacg gccagtgccc cacccggaaa gaagtcattt tgtaggtcga ctaagaggag
     2281 cgcggaatgt tgatcgggtt ggggaacggt ccaggtttga aaaggtgaag aatccgatgg
     2341 ggaggaatgc ttggaaagtt tcatattcgt tatgccaaaa tttcagtccc gatgcctttg
     2401 tctgtgaaaa tctcgagtaa caccgcatga ggcacccgtc catcaataat gtgcgcctta
     2461 cggacaccac cctgaagggc ggttaaacag gcatgaactt tgggaagcat gcctgcactg
     2521 atgacttgct ttttgaccat gcgttccatg tcttttctgg agagggtaga gacgtgccga
     2581 ttattggcat cacgaatgcc tttgacatcg cttaagacca gtaatttttc ggcttgaacc
     2641 gctgcagcta cgctaccggc taccagatcg gcattgatgt tgtgcgtttg ccatttggcg
     2701 tcgactccaa ttggcgcaat gaccggaatg aaattcgcct cttgaagcgt atgcagtaac
     2761 gtggtatcga catggctgac ttggccgacg aggccgtagt cttcagggtg cagcacgtcg
     2821 gttgatccgc tgtaggtgtg aataagactc ttgggattaa atggtttcgt ggtcaggagg
     2881 cgaccatcct tgccggtgag tccgacggct ttggcgccat gctggttcag caacgacacg
     2941 atttccttgt tgatttttcc accgaggacc atttcgacga cttccatggt tggctgatcc
     3001 gtgacccgaa ctccatgcag aaattttgcc tcaataccga atttatccag tatttcgtta
     3061 atttgtggcc caccgccatg aacgataatc ggttgcaatc cgacatattt taataagacg
     3121 acatcttcgg cgaagccttc cttgagttcc tcctggacca ttgcgcttcc cccatatttg
     3181 atgaccacgg ttttcccggc cagggtccga atatagggaa gtgcttcaat caggatgtcc
     3241 gcttttttaa tcagtttgtt catgcaacct actcatgggc tccagggttt gggggggaat
     3301 gagagaggca aaatggaaat atggccatca taacagatgt attgatttta aagaatgtac
     3361 cggctgaggt cttcgtcctt tacaatactt tggaggtgat cccgcacata ctgcgcgttg
     3421 atcacgacct ttttttcctg aagatcttgc gcttcaaacg agacatcttc caataaccgt
     3481 tccacgatgg tgaagagccg tcgtgcgccg atgttttcgg tccggtcatt cacctgcaca
     3541 gcggttgccg caatttcttc aatcccgtct ttggtgaatt ccaatgcgat cccttctgtg
     3601 ctcagtaacg cgtgatattg tttgaccagg gcatttttgg gctcggttaa aatgcggatg
     3661 aggtcatcct tcgtgagggg ctccagttct acacggatag ggaaccggcc ttgtaattcc
     3721 ggaattaagt ccgagggttt ggctacgtgg aatgctcccg cggcaataaa caaaatatga
     3781 tcggttttga ccgggccatg tttggtgttg accgtcgacc cctccacgat ggggaggagg
     3841 tcccgttgta ccccttcccg ggaaatatcg gggcctgtgt gtttctcccg tccggcaatt
     3901 ttgtcaatct cgtccaggaa gacgatacct gattgttctg tgcgggtaat ggcctccttg
     3961 gtgacttctt ccatgtcaat aagtttttgc gcttcttcct gggccatgaa tttcagggct
     4021 tctgagactt tcatccggcg atttttcttt tttcccggca tcataccgcc gagcatatcc
     4081 cgaaggttgt gctcaagatc ttccatgccc ccgatattgg aaatgattcc aaccggaagt
     4141 cctcctcgtt ctttaatttc aatttccacc atccgattat ccagtttgcc ttcgcgcagc
     4201 tggaggcgga gtttggagcg ggtggagtcc tgctgatcct gtttctgttg tgtgggagac
     4261 cctcctgact cctcagtgtt ttcaaacgag gggctcgagg gcgagggcgg aagcagcata
     4321 tctaagaccc gctcttcggc catgtcttca gccttttttt gaaccttttc cagataggcg
     4381 gccttgacca tattaatgga tagatcggtg aggtcacgaa taagagattc tacgtccctg
     4441 ccgacgtacc ctacctcggt aaatttggaa gcctcaactt taatgaatgg tgcatctgct
     4501 aattttgaga ctcgtcggga aatttctgtt ttcccgaccc ccgtaggacc aatcatgatg
     4561 atatttttcg ggacaatttc atcccggagt tcggggctga gctgttggcg ccgccagcgg
     4621 tttcgtaagg cgatggcgac catgcgcttg gcgtcccgtt gtccaatgac gtagcggtcc
     4681 aactcctcta cgatttggcg tggggtgagg gagtccaggt ttttgggtgt tggtgaagtc
     4741 tcttgttcca tacgttatga cgataactcc tcaataacaa tgtgatggtt ggtataaatg
     4801 tcgattccgg cggcgatttg catgctctgt tcgacaacct gggacggttg cagttcggtg
     4861 tgagccagga gggcgcgagc agcagataac gcgtaaggtc cccctgatcc aatggccaaa
     4921 atcccgtctt ccggttcaac cacatcacct gtgccggtaa taagaaatga atgctcgcgg
     4981 tcggccaccg ctaaaagcgc ttctaatcgt cgaaggaccc gatcggttcg ccaatccttc
     5041 gccagttcaa cggcggctcg ggtcagattt cctcgatact cttccagctt tgcgtcgaat
     5101 ttttcaaata aggtaaacgc atctgcggtg gccccggcaa agccggtgag caccttgtct
     5161 tgatgcattt tccggagttt cttggcgtta ctcttcataa tggtgctgcc gacggtgact
     5221 tggccatcac agcccatggc cactgtaggc cccttgcgta cgcacagaat cgtcgtggat
     5281 cgaatcttca tcgcttgaga gg
//
LOCUS       DATIAL010000295         2629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1237969, whole genome shotgun sequence.
ACCESSION   DATIAL010000295 DATIAL010000000
VERSION     DATIAL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237969"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(528..1565)
                     /locus_tag="VLA60_09270"
     CDS             complement(528..1565)
                     /locus_tag="VLA60_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09594.1"
                     /translation="MNRVVNHSQAMRTSLFGAVLFFMVFIGTVPASNGRSLNEPDPVS
                     GHSISLKEITPADVLARVQLLRDTLEMIRFEMGKPLATQSEAIVTNVHSHEVYFQALT
                     LFHKADRLSLELTGSTGISPEPTSLSTLAPLHIWKLVNASYLRILAIKEELGLKEIIS
                     ERPQDPSTSLTETGRAIVKANRQINLLQERHFSPSDVSQQVLLASQYAKRLLARFPQL
                     KEASETPPLERGKQPVDVFLRLVECYAILETIAHRSGIPVLHLDLPAAKNVGHSRQME
                     PSDVYDMATLLVSDLAYLHAQMKSHDLPKPIPYPGRTFPSHVYQQATVLLHQLEALEQ
                     MVGTDPTWLSR"
     gene            complement(1615..2241)
                     /locus_tag="VLA60_09275"
     CDS             complement(1615..2241)
                     /locus_tag="VLA60_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09595.1"
                     /translation="MIQLLQREILTNLGRACLLLVLLGYGGGSERAYGDSLNDDLVDL
                     TGGVIPLLSYDSGTSFSGGGELFSGGRLSSRGDVRYLVRLKNQTGDPIEADSLILVVD
                     KIQEMAQLRDVTTSLDIQGTDGNTEDGKPYFRVPVAGQAELPPYGESEPFLIEIKNPD
                     LLRLYPPVLRVRGIRKTASHVFQGALQNLIQEGVVTPEEAKKSMDQLR"
     gene            complement(2295..>2629)
                     /locus_tag="VLA60_09280"
     CDS             complement(2295..>2629)
                     /locus_tag="VLA60_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HSF09596.1"
                     /translation="LGIPQQTEDFGQTLGHYGLNPGAYLVLPIFGPSSMRDTAGLLTD
                     SAGSIFYLYTPLQLNYHFERSVPFSLTWGVDTRHQASFRYYQTGSPFEYDLIRFLYTK
                     YRELEIAK"
BASE COUNT          652 a          618 c          712 g          647 t
ORIGIN      
        1 cacaataagg caagccaaaa ggggaggaat tcgactcaac ttctgacgag gtaagccaat
       61 gggtaacaaa taggaggaaa gggatttggt caatgcccgt ctttcttcaa tcagaatcga
      121 ataccacgaa cccattggtg atatggacga ataggcctgt tgcggtaagt ttctcgaaaa
      181 agaatggctc ctgtgaccaa gagaatgttc atagcaagaa aaacaccacg atccggtttg
      241 aagcaccctc agtttcaggt tgcgtacttt tcaatttctc attttgcgtt ttatagcgga
      301 accggatcag atcatactcg aaaggtgaat ccgtttggta ggggcaaaag gtcaacaggg
      361 gggacgggct ctttagcttt cccgtcactt caaaaatcct gtgcttctgg gatattttcg
      421 atctcgtcga gggacgaggc ggagcataac caattaccat tctgggttaa gagatgggat
      481 atcgttgaag aagtatggat gaaatatttt caattgaggt ttattcttta gcgggaaagc
      541 cacgttggat cggttcccac catttgttcc agagcctcta gttgatgaag aagcacggtg
      601 gcttgctggt agacatgtga agggaacgtg cgtcccggat agggaatggg ttttggaagg
      661 tcgtggcttt tcatttgcgc atgaagatag gccaagtccg aaaccaggag agtggccata
      721 tcatagacgt cgcttggttc catttgccgg gaatggccga catttttcgc ggccggcaga
      781 tcaagatgaa gaacaggtat gccggaccga tgcgcaattg tttccagaat ggcatagcat
      841 tcaaccaggc ggaggaacac atcgaccggc tgcttgcccc tttcgagcgg gggagtctcc
      901 gaagcctcct ttaattgggg aaaccgggcc agcaaccgtt tcgcatactg actggccaga
      961 agcacttgtt gtgacacatc actgggtgag aagtgtcgct cctgcagtaa gttgatttgg
     1021 cggttggctt tcacaatggc ccgcccggtt tctgtcagac tggtcgaggg atcttggggg
     1081 cgttccgaaa taatttcctt caggcctaat tcctccttga tggccagaat acgaaggtag
     1141 ctcgcgttca ccaatttcca gatatgcaag ggtgcaagag tggagaggga ggtgggttcc
     1201 ggggaaattc ctgtactccc ggtgagttcc agcgagagac ggtcggcctt atgaaagagc
     1261 gtaagggcct ggaaatagac ttcatgggag tgcacattgg tgacgatggc ttcggattgg
     1321 gttgccagcg gttttcccat ttcaaaccgg atcatttcca gggtatcacg aagcagctga
     1381 acccgggcca ggacatccgc aggggtgatt tccttgagag aaatggaatg tccgcttacg
     1441 gggtcgggtt cattcaggct tcttccgttt gaggcaggca ccgttccgat gaataccatg
     1501 aagaagagca ccgcaccgaa taggctggtt ctcatggcct gactatggtt aaccactctg
     1561 ttcatctctt cctgattcct gcggattttc tccgggcact gacgttgctt gttgctatcg
     1621 taattggtcc atggactttt tggcttcttc cggggtcacg accccttcct ggatcaaatt
     1681 ctgcagcgca ccttgaaaca catgagaggc ggtcttgcgg attcctcgga ctcgtaacac
     1741 cggagggtat aagcgaagaa ggtcaggatt cttgatttcg atgagaaacg gttcgctttc
     1801 gccgtatggg gggagttctg cctggccagc aacgggaacc cgaaaatacg gtttcccatc
     1861 ttcagtattc ccatcggtcc cttgaatatc gaggctggtg gtcacatcac ggagctgtgc
     1921 catttcttga attttgtcca cgacgaggat caaagagtcg gcctcaatgg gatcgccggt
     1981 ctgatttttc agtcggacca gataccggac atcgccacga gaggaaagcc ttcctcccga
     2041 aaacaattcg cctccgcctg aaaaacttgt tccggaatca taggaaagca atggaatgac
     2101 tcctcccgtg agatcgacga ggtcatcgtt caaagaatct ccataggctc tttcactgcc
     2161 ccctccataa cccaaaagaa ccagcagcag gcatgcgcgg ccaagattcg tcaggatctc
     2221 cctctgcagc aattgaatca tcgtggcgtc atcttacaaa gaacgtgatg acccggcaaa
     2281 ggtgcccata ccggttactt ggcaatttct aactcccgat atttggtgta gagaaaccga
     2341 atcaggtcat attcgaacgg agatccggtt tggtaataac gaaaactcgc ctgatgcctc
     2401 gtgtccaccc cccatgtcag actaaaggga acactacgtt caaaatgata atttaattgc
     2461 aagggagtgt acaaatagaa gatgctgccc gcggaatctg tcagtaaccc ggcggtgtcc
     2521 ctcatagatg aaggtccgaa aatgggaagc acgagatagg ctccgggatt gagtccgtaa
     2581 tgcccgagcg tttgtccgaa atcctctgtc tgttggggta tacctaagg
//
LOCUS       DATIAL010000296        11864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_485436, whole genome shotgun sequence.
ACCESSION   DATIAL010000296 DATIAL010000000
VERSION     DATIAL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485436"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..948
                     /locus_tag="VLA60_09285"
     CDS             511..948
                     /locus_tag="VLA60_09285"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HSF09597.1"
                     /translation="MITAQYPRSQKEQVGGLCHLGRLIDKVRMRNAGQIQDYNYLTVG
                     FDKYLLDKLEIKGEDFEKRVLQGGTDAEIADWVKSNGKNLSDAEKAEWNDMVLTFGPK
                     APMAQKAFDEYKSGLANKRGVSVESLSHITTWCSLIEHDEGRK"
     gene            complement(1013..1459)
                     /locus_tag="VLA60_09290"
     CDS             complement(1013..1459)
                     /locus_tag="VLA60_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09598.1"
                     /translation="MSTRRFSRFSRLFHLSFAACLGLGLTAGIAQAQLETEPHPLDTF
                     IKGITNFSQPTGIRGTVRYIEKDRQTLWLNWEQRSDERPLFDSGWKLVPGDATLAVQP
                     RDSEQWEKLSNLSKGLPLELIVQDDGKGHRHILSFEDISGPPKIPL"
     gene            complement(1812..2471)
                     /locus_tag="VLA60_09295"
     CDS             complement(1812..2471)
                     /locus_tag="VLA60_09295"
                     /inference="COORDINATES: protein motif:HMM:NF025468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HSF09599.1"
                     /translation="MKKLFIFVEGQTEEKFSKELLLPYLESKGIFLQPIISTTKVVKD
                     GKNFRGGLGPNPRIKKEICNLLKDKSARAVSTMLDYYGLPKSFPGRSNPVGHTCFDRA
                     KFVEQSMLADFNDERFIPFLTLHEFEGLLFSSPADMAAGLPGGQNLEAPFKIIRQQFE
                     SPEEINDQPSTAPHQRILNIYPTYQKPTFGVAIASRIGLEKIREQCPHFSDWLTTLEQ
                     L"
     gene            complement(2474..3547)
                     /locus_tag="VLA60_09300"
     CDS             complement(2474..3547)
                     /locus_tag="VLA60_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF09600.1"
                     /translation="MKLEKIILKGFKSIRELELDLRPLNVLIGPNGAGKSNFIGAFKL
                     LNEMVEGNLQNSIARGGGAGTFLHFGEKVTGKIEVELHFGANGYYCVLAPAADGSLFF
                     EKEQCLFWGGSVSRHSESLGVGHKESKLSPTLSKIASHVSPALKSWKVYHFHDTSDTA
                     KVKSIGDINDNFYLRADASNLAAFLYTLKNGLFKSYYEAIRDQVRQVAPFFDDFILRP
                     DPSNENKIRLEWREKGSDYPFMAYHLSDGTLRFMCLATLLLQPNPPSTILIDEPELGL
                     HPYAITVLASLFHGLPTNTQLIVSTQSVPLVNQLQLEDIIVVDRIDGQSTFRHLQPEE
                     MADWLDDYGLGDLWEKNLLGGRP"
     gene            complement(3630..5093)
                     /locus_tag="VLA60_09305"
     CDS             complement(3630..5093)
                     /locus_tag="VLA60_09305"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HSF09601.1"
                     /translation="MRHRPPLHIRQGYTSTSPLPTQVVSSEEFLPPPQSIKQSQVEWL
                     IHQSSNRLSSRLGMNRRDFLKTTGGMALAFLAMNQVFGKFFDVLDVEAAEPQAVQTRK
                     GNIPFIFDVQTHYVSSSFNQPGWKEGLLGLRRRAKEMGLNPKLSGDRGTMEDLSLENY
                     IKEVFLDSDTSIGLISTPPGPYPWEAVVPPKEMTHIRDEINRLTASQRMLAHGLVMPQ
                     LGKVDLDYMDQQAETFKVDAWKCYTGSPPKGFEHGWWLSDEKIAYPMLEKAQALNITN
                     VCAHKGLPLGPVPDYNHPRDILQAAKDFPKLNFLIYHSGFLGTSRINLDETKKGEIPW
                     TSELCRLKQKQPKLNNVYMELGSTFGQLVITEPVVCAHLLGQILLAFGEDHLLWGTDS
                     IWYGTPQWQIEAFRRFQIPEELQEKFHYPALTKDLKAKVFGLNAAKLFKVDVEAKRKD
                     VPKDYLSHIKMTYLDEGFSPSHHAYGWIQGSQPQLQV"
     gene            complement(5167..5574)
                     /locus_tag="VLA60_09310"
     CDS             complement(5167..5574)
                     /locus_tag="VLA60_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09602.1"
                     /translation="MTTAERFLVEGQPSFSLGLMGMLLLLPALLAAAALEQSSTQLSL
                     PDEDSYRSPLSENLLESNRWRIPQEEKRDWRLPPPPPPVGWRTPQSEESKTSPSRRTI
                     ELFPRYQPGKSSDYDMIEREEKPLIKMFEFGSK"
     gene            complement(5571..6326)
                     /locus_tag="VLA60_09315"
     CDS             complement(5571..6326)
                     /locus_tag="VLA60_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HSF09603.1"
                     /translation="MSPDSQPGTLQHVIESQRLRVGVSLFPPWTLKDSNDQLTGFEID
                     IARQLAKDLGVQAEFHVFEWQNIVPALLDRKIDIITAGMVITPQRALKVNFSDPYDSA
                     GIGLVTNIPLTKTFLGPQDLNQPEVIITAVTGTVAEDLARRVFPKATIKTFASSQDAI
                     QALTSGKVHGYVEHEPITTFMALEHPKTVDEPLSKPLLETKTGFAVNKGDPDFIHFLN
                     AWIISHNADTWLDSAHKYWFEGVEWRKHVGTNP"
     gene            6581..7987
                     /locus_tag="VLA60_09320"
     CDS             6581..7987
                     /locus_tag="VLA60_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HSF09604.1"
                     /translation="MKFRCFMSWIFLLVILCLVNVVSAEPVLQPWTKNPWYWSDHGEP
                     VLLLGGSDDDSLFQWPEKDLLAQLDRLVAAGGNVIRNTMSSRQDRGFEVFPFKQLNDG
                     RYDLEQWNDEYWARFDRMLRETAKRGIVVQIEVWDPWDYMGDTWHIQPYNPRNNITYT
                     MENSELAERYPEPPYLNKQPFFFTTPRQRNNRVVLGYQQRFVDKMLDYTLRYGHVLYC
                     MDNETNGEEEWSRYWATYLKHRAGKESKKVFVTEMWGDWDLTAEIHRRTFDHLEVYDF
                     VDVSQNNHQRGQKHWDQFLRTRHFLAKHPRPINTTKTYGATGNRFGNDQDGIERFWRN
                     LLAGAASIRFHRPTAGLGLNDKAVASIRGARKLESQIPLWTLEPANDLLIDTKPNHAF
                     LAADMGHAFALYLPVGGGVRIDLSSTAEPLVIRWIEIATGEWGPEQHMQGGGNADLTS
                     PGSGNWAAAITPERPGTD"
     gene            8092..9645
                     /locus_tag="VLA60_09325"
     CDS             8092..9645
                     /locus_tag="VLA60_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HSF09605.1"
                     /translation="MKRALAILGTGSDVGKSLVTAGLCRLLHRAGVRVAPFKAQNMSL
                     NSFVTTDGGEMGRAQVLQAQACGLAPHVDMNPILLKPEADAKSQVIVQGQVWRSQNAR
                     DYFERKSQLVEFVKESYERLSKQYDVMVIEGAGSAAEMNLRDRDIVNWPMVEMADAAV
                     VLVADIDRGGVFAQVIGTMDLLASKERQRVIGVVINKFRGDLTLFNDGVTIIEERTGV
                     PVLGVLPYLRNLELDQEDSVDIDRLRKTPFEADTVNIAVVLLPHMSNFTDFNQVAAEP
                     DVALRFVASPQELHGADAVILPGSKTTIADLEYLRKVGFEEALMTHVQRGNEMMGVCG
                     GFQMLGKEITDPQHVETGGSTKGFGLLEVKTELLAHKKTVQVRARSLPESWGHECLVE
                     GYEIHMGDTRTVNPISSCFRILTDQAPRSVEQPESRVDGAMSLDGRVWGTYIHGVFDQ
                     PAFRRQWLNRARVRKGLAPLGLEISEEVSQRMSQALDRWADHLEAHLDVNPIFSALGL
                     NGSSPDRHH"
     gene            9725..10651
                     /locus_tag="VLA60_09330"
     CDS             9725..10651
                     /locus_tag="VLA60_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSF09606.1"
                     /translation="MRYDKNFLWKLDEPGSGNHQSAWSGRPGSWWALAKEVWAASCED
                     DVFGRAAQLGFYFLLALFPAMLGVTALIGMLPRQVVVPNVMPYAREVLPAESLVLVER
                     YVEQMIQGSGGGLFSLSLLGSLWAASWGMMAIINTLNAVYGVKETRPILKAGMTAVLL
                     TLGAAVFFITSLILILAGEQVSYWMAHVTGMDWWVTFGWPLLQWPVIVFFMLMAINLV
                     YYWAPNIDHQWQWMKPGSMLAVFLWIILSLGLKFYVENFINYNAVYGPITGVIILMMW
                     LYVCGLTLLIGGELNFILQRPDTMDGSVSSET"
     gene            complement(10707..11528)
                     /locus_tag="VLA60_09335"
     CDS             complement(10707..11528)
                     /locus_tag="VLA60_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HSF09607.1"
                     /translation="MIGSEYYTLSPASIPLENLRIALLHLAPIPGDLTENRRLIERAI
                     TVAVGSGATWIITPELCVCGYTFTDRIGTDWIMPQPDRWMTRMCQLAARLHVTLFLSH
                     PERDPQSNTLYNTVFVIRPDGAIAGTHRKINTLPTGSELWSTPGTQVAPVSIPPFRNV
                     GILICADAFSPGIAKSLADQGAKMLISSAAWASGFHGPNGEWERCTQDTGLPLLVCNR
                     TGPDRTLDFRTAESVVVKDGKRLLTLSSDRSTAFIIDWNLHRQNRATQKYQQIAL"
     regulatory      11757..>11864
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2837 a         2622 c         3329 g         3076 t
ORIGIN      
        1 tcaatctgat cgaacgtgcc gtgccggtgc atcaagcggg gggacatcgg tatcctatct
       61 ataaagaagt tcgggcgggc tttccgtttg gcggaccaaa ggggtttgat cggtggtggc
      121 agtccgcttc tgcccacaaa gccagaaccg ttggcctcct gttggtgtag gttcccggcg
      181 ccccatggag atttccttga taagagggtg atggcgacag gcatttgtca gtcgggttgg
      241 aaacagatcc ttcgattctc tcaggataac aaacagataa ctgaggcagg tgccgccttt
      301 cttgtcatgc tgagcccatt cgaccgcgct cggggtaggc aaggcgcacc ccagcttccc
      361 caaagactgg cacagatgcc gagggttcac tacggtgagc tcgggacaaa aattattggc
      421 tatttcgttt tgtccctttc acaaggtata accagttcaa tacgcatttc ggtagactat
      481 ttctacacac tattattata aggaggcatc atgatcacag cacagtatcc acgcagtcaa
      541 aaagaacaag tcggcgggct ttgccatctc ggacgactta tcgacaaagt ccggatgcgg
      601 aatgccggac agattcaaga ctacaattat ctcaccgtcg gcttcgataa atatttgtta
      661 gataaattgg aaatcaaagg agaggatttt gaaaagcgcg tgctccaagg cgggacggac
      721 gcagagattg ccgactgggt gaaatctaac ggaaaaaacc tgagcgatgc ggaaaaagcc
      781 gaatggaatg acatggtgct gacattcggc ccgaaagccc caatggccca gaaagccttc
      841 gacgaatata aatccggtct ggcgaacaaa cgtggtgtgt cggtggagtc cctttcccat
      901 atcaccacgt ggtgcagtct catcgaacac gacgaaggcc ggaagtagcc gacagcccct
      961 tcagattttt atgcgggcat ctggtgcccg attggcgtgg aactcaaaag atctacagcg
     1021 ggattttcgg gggaccggaa atatcctcga aggagagaat atggcggtga ccttttccgt
     1081 cgtcctggac gatcagttcc agaggcagcc ctttcgaaag gtttgacagt ttttcccatt
     1141 gctcggaatc ccggggttgg accgctaagg tggcatcccc gggaaccagc ttccatcctg
     1201 agtcaaacaa cggtctttca tctgatcgtt gttcccaatt taaccagagt gtctggcggt
     1261 ccttttcgat atatcggacg gtgccacgga tacccgtggg ttgagaaaaa ttggtaatcc
     1321 cttttatgaa ggtgtccagc gggtgaggtt cagtttcgag ctgtgcctga gcaatgccag
     1381 ccgtcaaccc caatcccagg catgcggcaa agcttaaatg gaagagtctg gaaaaccgtg
     1441 agaaccgtcg tgtcgacatg gataacctca caggtgagag gaaggatgac cttttaaact
     1501 gtttaattta atcataagca gagacgattc acaaatgccg ggcttttgaa tttgaaactc
     1561 caattgaaaa tttggatttg taaaagagtc aaaacctcaa aggtttaaat ttctttatga
     1621 gggctggagt atggagccag agagttttca tctttggtcc caacggtgaa cagatcaaga
     1681 aagtctatgg tggctataag atgaaaactt cgaaaagtca ggaaattttc agtgaaccaa
     1741 ctttagaaga gctgattcat acaccttcaa tgggacgagg ataggatgta catatcttta
     1801 tacgttttct ttcagagttg ctcaagagtt gtgagccaat cagagaaatg aggacattgt
     1861 tctctaattt tctccaaccc tattcttgaa gctatagcaa ccccaaatgt aggtttttga
     1921 taggtagggt atatatttag aattctttgg tgaggagcag tgctaggttg atcgttaatt
     1981 tcctcaggtg attcaaattg ctgacgaatt attttaaatg gagcttctaa attttggcca
     2041 cctggtaatc ctgccgccat gtctgctggg gaagaaaaaa ggagtccttc aaattcatga
     2101 agggttaaaa acggaataaa tctttcatca ttaaagtcgg caagcattga ctgctcaaca
     2161 aacttagcgc gatcgaagca agtatggcca acaggattgg atcgtccagg aaagctttta
     2221 ggaagcccat aatagtccag catcgtagag actgctctag cacttttgtc cttaagaaga
     2281 ttgcaaattt cctttttgat tctaggatta ggtcctagtc cgcccctgaa atttttaccg
     2341 tcctttacaa ctttagttgt agaaatgatg ggttggagga atattccctt actctccaag
     2401 taagggagga gtaattcttt ggagaatttt tcttccgttt ggccttcaac gaaaatgaat
     2461 aattttttca cctttaagga cggcctccaa ggagattttt ttcccagagg tctcccagcc
     2521 cgtagtcatc aagccaatct gccatttctt ctggttgcaa atgccggaat gtcgattgac
     2581 catctattcg atcaacgaca atgatgtcct caagttgaag ctgattgact aatggtacgg
     2641 attgtgtgga aacgattagt tgggtgttag ttggtaggcc atgaaataaa gatgcgagga
     2701 ctgtaatggc ataaggatga aggcccaatt ccggttcatc aattaaaatt gtggatggcg
     2761 ggtttggttg cagtaataat gttgccaaac acatgaagcg gagtgttcca tctgaaagat
     2821 gataggccat aaaggggtag tctgaccctt tttctctcca ctcaagacgg attttatttt
     2881 cattagatgg gtctggcctt aaaataaagt catcaaaaaa tggagcaact tgcctgactt
     2941 ggtcccttat ggcttcatag taacttttga ataatccatt ttttagtgtg tataaaaatg
     3001 cagctaaatt agatgcatct gctctgaggt aaaagttgtc attgatgtcc ccaatgcttt
     3061 ttacctttgc ggtatcactg gtgtcgtgaa agtggtagac cttccagctt ttgagggcgg
     3121 gggaaacatg gctagcaatc tttgaaaggg ttggagataa tttgctttct ttgtgaccta
     3181 cacctagtga ttcggaatgt cgacttacag aaccacccca aaagagacat tgctcttttt
     3241 caaaaaaaag agagccgtct gctgctggag ccaaaacgca ataatatccg ttggccccaa
     3301 agtgcagttc cacctcaatt ttgcctgtga ccttttctcc gaaatgtaaa aatgttcctg
     3361 ctccaccacc tcgggcaatt gaattctgaa gatttccctc caccatttca tttaacagct
     3421 taaaagcacc aatgaaattg cttttacctg ctccattggg acctattaaa acattcaggg
     3481 gacgtaagtc tagttcgagt tcacggatgg atttgaagcc tttgagaatg attttttcga
     3541 gtttcattta tcagtttaat cgaaaaataa tattaatgaa tttactttta ttccagaaat
     3601 ggccgaattt ctttttgcca tcacctaagt caaacttgga gttgtggttg ggatccttga
     3661 atccagccat atgcatggtg actgggggag aatccttcat ccaggtaggt cattttgatg
     3721 tggctgagat agtccttcgg gacgtctttc cgtttggctt cgacatctac cttaaacagt
     3781 ttagccgcat tgagcccaaa gactttcgct ttgagatcct tggtcaaagc agggtaatgg
     3841 aacttttcct ggagttcttc aggaatttga aaccgccgga aggcttcgat ctgccattgg
     3901 ggtgtgccat accaaattga atccgttccc cagagaagat gatcttctcc gaacgccaaa
     3961 agaatctgtc cgagcaggtg ggcgcagacg acgggttcgg tgatgaccag ttgcccgaag
     4021 gtcgagccca gttccatata cacattgttg agcttcggtt gcttctgttt taatcggcac
     4081 aattcgctgg tccagggaat ctcacctttt ttggtttcgt caagattgat acgagatgtg
     4141 cctagaaatc cggaatggta aatcaggaaa ttcagtttgg ggaaatcttt cgcggcctgc
     4201 aggatgtcgc gtggatggtt gtaatcgggc accgggccga gcggcagacc tttgtgcgcg
     4261 caaacgttgg tgatgttgag ggcctgcgct ttttccagca tggggtaggc gatcttttca
     4321 tcgctcagcc accagccatg ctcgaagcct ttcggcggcg agccggtata acacttccag
     4381 gcatcgacct tgaacgtctc cgcttgctgg tccatataat caagatcgac cttgcctaac
     4441 tggggcatga ccagcccatg ggcgagcatg cgttgggaag cggtgaggcg attaatttca
     4501 tcacggatat gcgtcatttc tttgggcggg acgacggctt cccatggata ggggccagga
     4561 ggagtactga tgagcccaat tgaagtgtcg ctgtccagaa agacttcttt gatgtagttt
     4621 tccaggctca ggtcttccat cgttccacga tcgccggaga gcttgggatt caggcccatt
     4681 tcttttgccc gccgtcgcag gcccaggagt ccctctttcc agcccggctg gttgaaggag
     4741 gaactgacgt agtgcgtttg cacatcgaag atgaacggaa tgttcccttt gcgtgtctga
     4801 accgcttggg gttccgccgc ttcaacgtca agcacatcga agaattttcc aaacacctga
     4861 ttcatggcca aaaaagccag tgccattcca ccggtggttt tgaggaaatc ccgccggttc
     4921 atgccgaggc ggctgctcag ccggttgctg ctttggtgga tcagccactc gacctggctt
     4981 tgtttgatgg attgtggggg cggtagaaat tcttcgctgg agacgacctg cgtgggaagg
     5041 ggtgaggtcg aggtgtatcc ttgccgaatg tggaggggtg gtcgatgcct catatgatca
     5101 attctgcctt ttccatggcg ccgaaggcaa acacggcaga ggtgaatggt ggcagcggcg
     5161 cagaaattat ttcgatccga attcaaacat cttaatcaac ggtttttcct ctcgttcgat
     5221 catgtcgtag tcggatgatt tccctggctg gtatcgaggg aacagctcaa tggttcgtcg
     5281 cgagggggat gttttcgatt cctcggattg tggggttcgc cagcccacgg gaggaggggg
     5341 aggtggtaat cgccagtccc gtttctcttc ttgtggaatt ctccatcggt tggattcgag
     5401 gaggttttcc gagagaggag atcggtacga gtcttcatcc gggagcgaca gctgggtgct
     5461 ggattgttca agggcagccg ctgcgagcag tgccggaagg aggagaagca tgcccatcaa
     5521 ccctaaggag aaagagggtt gtccctcaac aagaaaccgt tctgcagtcg tcatgggttc
     5581 gtgcccacat gctttctcca ctcgactcct tcgaaccagt atttgtgagc ggagtccaac
     5641 caggtatcgg cgttatggct gataatccac gcgttgagaa agtgaatgaa gtcggggtct
     5701 cctttgttga ccgcaaaccc cgtcttggtc tcgagcaacg gtttggataa tggttcatcc
     5761 acggttttag gatgttccag ggccataaaa gtcgtaatgg gctcgtgctc gacgtagcca
     5821 tggacctttc cgcttgtgag ggcctgtatg gcatcctgac tggaggcgaa ggttttaatt
     5881 gtggcttttg gaaatactcg ccgggcgaga tcttccgcca cggttccggt cacggcggtg
     5941 atgatgacct ccggttgatt caggtcctgg gggccaagaa aggttttcgt taacggaata
     6001 ttggtgacca gtccgatccc ggcagaatca taggggtcgc tgaagttgac ttttagggca
     6061 cgttgcgggg taatgaccat gccggccgtt ataatatcga tcttccgatc caggagagcc
     6121 ggcacaatat tctgccattc gaagacatga aactctgctt ggactccgag gtctttggct
     6181 aattgacggg cgatatcgat ttcaaagccc gttaattggt cattgctgtc tttgagggtc
     6241 caggggggaa atagtgagac acccactcgt aatctctgac tttcaatcac atgttgcagg
     6301 gtgccgggtt ggctgtcggg cgacatgctc gatggggatt ttgcggggag ctcagcccgg
     6361 gctgctgcca gaatgaccag ggaccagcag aggacggctg cgaaacaatt cagtagggtg
     6421 cgacaagacc gttggtgtgt gtacattatt accgccatgg ttgtggttcg cgtttatgaa
     6481 aggtgtcact gtaccatagg gcttctatcc ctgaaagtag gtggatagca gtcgaatggt
     6541 aaccaaaaaa cagaaggggt catgatggcg ggagggacat atgaaattta gatgcttcat
     6601 gagctggata tttttgttgg taatactttg tttggtcaat gtcgtatcag ccgagcctgt
     6661 gttgcaaccg tggaccaaaa atccctggta ttggagtgat catggagaac cggtgttact
     6721 gttggggggg agtgatgacg acagtctgtt tcaatggccg gaaaaagatc ttcttgcgca
     6781 gcttgatcga ttggtggccg ctggtggcaa tgtcatccgg aacacgatga gcagccgaca
     6841 ggatcggggt tttgaggtct tcccgtttaa acagctgaat gatggccggt acgatttaga
     6901 gcagtggaat gacgagtact gggcgcgctt cgaccggatg ctgcgggaga cggctaagcg
     6961 cggtatcgtt gtgcagatcg aggtttggga cccttgggac tacatgggcg acacctggca
     7021 tattcagccg tataacccac gtaataatat cacttacacg atggaaaatt ccgaattggc
     7081 agaacgatat cccgaacccc cctatctcaa caaacaaccg tttttcttta cgacgccaag
     7141 gcagcgaaac aaccgtgtgg ttttggggta ccaacaacga tttgtcgata agatgctgga
     7201 ttataccctg cgatatggtc atgtgctcta ttgcatggac aacgaaacaa atggggagga
     7261 agaatggagc cgttactggg cgacatacct caagcatcgg gcgggtaagg aaagtaaaaa
     7321 ggtttttgtt acggagatgt ggggggattg ggatctcacc gccgaaattc atcggcggac
     7381 gttcgatcat ttggaggtct acgacttcgt cgacgtatcc cagaataatc accagcgcgg
     7441 acaaaagcat tgggaccaat ttctccggac gcggcatttc ctggccaaac atccgagacc
     7501 catcaatacg accaagacct atggtgccac cggtaaccga ttcggcaacg accaggatgg
     7561 aatcgaacga ttctggcgga atctgttggc cggtgccgca tccattcgtt ttcatcgccc
     7621 cacggcagga ctgggactca atgacaaggc cgtggccagt attcgtgggg cgagaaagct
     7681 ggagtcgcaa ataccattat ggaccctgga acccgcaaac gatctgctca tagacaccaa
     7741 gccgaatcac gcgtttttgg ctgccgacat gggccatgcc ttcgccctct acctgcccgt
     7801 tggcggtgga gtgcgaattg atttgtccag cacagcggag ccgttggtaa tccgttggat
     7861 cgaaattgcc acaggtgaat ggggccccga acaacatatg caaggcggcg ggaatgctga
     7921 tttgacgtct cctggctcgg gcaattgggc tgcggcaatt accccggaac gtcctgggac
     7981 tgactgagga agctgattaa gcaaagggtg tcccgaacgg aggtctgtgt tctctacaat
     8041 gcggcacact tgaaggacgt ggttgcactt aatccgaaga agaaggcata gatgaagcgg
     8101 gcattggcta tattgggaac cggctccgat gtgggaaaaa gtctggtcac ggccggattg
     8161 tgtcgactat tgcatcgggc cggggtcaga gtggcaccgt ttaaagcaca aaatatgtcg
     8221 ctgaattctt ttgtcacgac ggatggggga gagatgggac gggctcaagt gcttcaggct
     8281 caggcctgtg ggttggctcc gcatgtggac atgaatccga ttttgttgaa gccagaggcc
     8341 gacgccaaat ctcaagtgat tgttcaagga caagtgtggc gaagccagaa tgcccgggat
     8401 tatttcgagc ggaaatccca gttggttgaa tttgtgaagg agagttatga acggctgtcc
     8461 aagcagtatg acgtgatggt gattgaaggc gcggggagtg ccgcagaaat gaacctgcgg
     8521 gatcgcgata ttgtgaactg gccgatggtc gagatggccg atgcggcagt ggtgttagtg
     8581 gcggatatcg atcggggggg tgtgtttgcg caggtcatcg ggacgatgga tttattagcg
     8641 tcgaaagaac ggcagcgggt gatcggggtg gtcattaata agtttcgagg ggatctgacg
     8701 ttatttaacg atggtgtcac catcatagaa gaacgcacgg gcgtcccggt cttaggcgta
     8761 ttgccgtatc tacggaatct ggaattggat caagaggaca gcgtggatat tgatcgattg
     8821 cgaaagaccc cttttgaagc agacaccgtt aatatcgcag tggtgttact gcctcatatg
     8881 agtaacttta cggatttcaa tcaggtggct gcggaaccgg atgtggccct tcgctttgtg
     8941 gcgtcacctc aggagcttca tggggcggat gccgttattc tgccgggaag caaaacgacg
     9001 attgccgatt tggaatatct tcggaaggtc ggatttgaag aggcgcttat gacgcatgtc
     9061 caacgtggaa acgagatgat gggtgtctgt gggggatttc agatgctagg gaaagagatc
     9121 acggatccac agcatgtgga aaccggtggt tcgacgaaag gattcgggtt gctggaggtc
     9181 aagacggaat tgcttgctca caaaaaaacg gtgcaagtcc gtgcccgttc cttaccggag
     9241 tcctggggcc atgaatgtct ggttgaaggg tatgaaatac atatggggga cacgaggact
     9301 gtgaatccca tttcatcctg ctttcggatt cttacggatc aggcgccacg ttctgttgag
     9361 cagccagaat caagggtcga tggagcgatg agtctggacg ggcgggtctg ggggacctac
     9421 attcatgggg tctttgatca acctgcattt cgtcgtcaat ggttgaatcg ggcgcgggtt
     9481 cgcaagggcc tcgcgccact tggcttagaa atttcagagg aggtttcaca gcgaatgtca
     9541 caggcattgg atcgttgggc tgaccatcta gaggcccatc ttgatgtgaa tcccattttt
     9601 tcagcattgg ggttaaacgg ttcttctcca gaccggcacc attaatcgaa atgaagaggt
     9661 gttgattttt ccagaaacct gttttacgca gatcatatcc gggtatgggg tataataaat
     9721 accgatgagg tacgataaaa attttttatg gaaacttgat gagccgggtt cgggcaatca
     9781 tcaatcggct tggtccggaa ggcctgggag ttggtgggca ttggcgaaag aggtgtgggc
     9841 cgcctcatgc gaggacgatg tgtttggacg ggcggcacag ctcgggttct attttttgtt
     9901 ggcgttattt cccgccatgt tgggagtgac ggcgctcatt ggcatgttac ccagacaggt
     9961 ggtcgtgccc aatgtcatgc catacgcccg ggaagtgctt cccgcagaat ccttggttct
    10021 cgttgaacgc tatgttgaac agatgattca gggcagtggg ggtggacttt tttccctgag
    10081 tcttctcggc tcactgtggg cggcttcctg gggaatgatg gcgattatta ataccttaaa
    10141 tgctgtctat ggggtaaaag aaacccggcc gattttgaaa gccgggatga ctgccgtcct
    10201 gctgacgcta ggagctgcgg tattttttat tacgtcgtta atcttgattc tggcgggtga
    10261 acaggtgagc tattggatgg cgcatgtcac cggaatggac tggtgggtga cctttggctg
    10321 gcctctgttg cagtggccgg tcattgtgtt ttttatgctg atggctatta acctggtcta
    10381 ttattgggcg cctaacatcg atcatcaatg gcagtggatg aagccgggct ccatgcttgc
    10441 ggtttttctc tggattatcc tatccctggg gctgaaattt tatgtggaga atttcattaa
    10501 ttacaacgcg gtctatggac ccattaccgg agtgattatc ctgatgatgt ggctgtatgt
    10561 ctgtggtttg actcttttga taggaggaga gcttaacttc atcctccaac gtcctgacac
    10621 catggatgga tctgtttcgt cagaaacttg acccgtgaat ctgtgcgtct ttgtttaaac
    10681 cccttgcggc ctgtccctta gtaatttcac aaggcaatct gttggtactt ctgtgtcgct
    10741 ctattttggc gatgaagatt ccaatcgata ataaaagccg ttgagcgatc cgacgatagg
    10801 gtcaggaggc gcttaccgtc cttgaccaca acactttccg ctgttctgaa atcgagtgtc
    10861 cgatcaggtc cggtgcgatt gcacaccagt agggggagtc cggtgtcctg agtgcatcgc
    10921 tcccattccc cattggggcc atgaaatcct gaggcccatg cagcggaaga aatcagcatt
    10981 ttggctcctt gatcggcaag acttttcgct attcccggcg agaaggcatc agcacagata
    11041 agaatcccca cattgcggaa gggcgggatg gatactggtg ccacctgagt gccgggagtt
    11101 gaccacaatt cagagcctgt cggcaatgtg ttgattttgc ggtgtgtgcc tgcgatggcg
    11161 ccatcgggcc taatcacaaa gacagtgtta taaagggtgt tggactgggg atcccgttcc
    11221 ggatgggaga ggaaaagggt cacatgcaac ctggctgcga gttggcacat gcgggtcatc
    11281 caccggtcgg gttgaggcat gatccaatcg gtgccgatgc ggtcggtgaa tgtgtaaccg
    11341 cagacacaga gttccggagt aatgatccag gtggctcctg atcctaccgc gaccgtgata
    11401 gcccgttcga tgagccgcct gttttcggtg agatcaccag gaattggtgc gagatggagc
    11461 aacgcgatgc gcagattttc caatgggatg ctcgcggggg aaagtgtgta atattcggaa
    11521 cctatcacat gtcaatagac gcttgcatca tgaggcggat tgcaatgcgt cgatacgtcg
    11581 atgaaatcta gtcgttgtca ctggcgagct ggaatggaat cccctattcg tgagcccggt
    11641 gagtccaaga gcttcgttta tggggtgggt ggattgcaaa ggccggtttg ggggatcgtt
    11701 aaaacagaca accttgcgcc ggccgacgaa cactgaaatg cgatttcttg acaaagcggc
    11761 tccgtccctc ctacattctc ccaaaacata tgcggaatga atgatgggaa gcgggtgcaa
    11821 ttcccgcgcg gtcccgccgc tgtaactgag gacgaacccc gcga
//
LOCUS       DATIAL010000297         7207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_271833, whole genome shotgun sequence.
ACCESSION   DATIAL010000297 DATIAL010000000
VERSION     DATIAL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271833"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VLA60_09340"
     CDS             complement(<1..194)
                     /locus_tag="VLA60_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09608.1"
                     /translation="MNLKSGQWEEASLPSFYHKRNGVSPALARLGMVKGEVPLPFMRR
                     VRYLHVERVRFDGLFGGIHSG"
     gene            210..1166
                     /locus_tag="VLA60_09345"
     CDS             210..1166
                     /locus_tag="VLA60_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSF09609.1"
                     /translation="MTPSELNASPLTYRLKLGLVLNGIIILAEFIGGYAINSLGLMSD
                     AGHNLIDQGSLLLALYAHILATQPATEHRTFGYHRAGTIAAFLNSLLLIFTGGIIGVF
                     ALDRMFSPVDVPGFWVMMIACISLIANLAVALLLRRGAEDDLNIRGAFLHMVMDAWMS
                     LGVIIAGLGIALTGFTILDPLISLLIVIIIVKGSWPLFRESLDILLESTPPHLKTSDI
                     VATIENVPGVRKVHDLHIWSVEPRIVMLTCHVLVDPEAVPDKDQLLQPIQSILSSKFG
                     IHHLTIQLETECQEQEDLHCNLSYLTNGSTGAEPIPPHLHHH"
     gene            complement(1163..1705)
                     /locus_tag="VLA60_09350"
     CDS             complement(1163..1705)
                     /locus_tag="VLA60_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSF09610.1"
                     /translation="MNFDELWFASINGWAGQFAGLDWFMLQVSQESNLVIPGILLVGY
                     WGWMKWGEARLAIPCLGLLIGLSDFLGGQMKVLISRPRPCQVLEHIHELVGCGGAFSM
                     PSNHALNSSTAISFLVMLYPGLGWVLWPLLGLIGLSRVFLGAHYVTDVLVGVGLGALL
                     GGGVGFLLKTLVFRRKPLAG"
     gene            1813..2595
                     /locus_tag="VLA60_09355"
     CDS             1813..2595
                     /locus_tag="VLA60_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HSF09611.1"
                     /translation="MLIDTHVHLDDPRYDPDRDTIFQRAQEAGVEKFVTIGCDLSTSH
                     AAVQLATSRPDVYATIGVHPHEVKLIEPNWYAEFRQLAQHPKVVAFGEIGLDYHYDHS
                     PRETQRQRFREQIELAQSLTLPLVIHTREAQEDTMAILQEAHAEKVGGVFHCFSEDLA
                     FAKRALDLGFHLSFSGIITFRNASQLHEVIRTVPDDRLLIETDAPYLTPVPFRGKRNE
                     SSYVTYVAEQIAKIKYGDSETGLARIAELTTNNACRLFKISS"
     gene            complement(2579..3124)
                     /locus_tag="VLA60_09360"
     CDS             complement(2579..3124)
                     /locus_tag="VLA60_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HSF09612.1"
                     /translation="MAPKHLIIDGYNVLGAMGLPPHRVVEQGEHRREEFIVRVGLYGH
                     KLHNLITLVFDAWQQSGASRQVIHRNGVTVIYTGQGEKADGVIQDFIRSHGKGAAVVS
                     SDLEVIAVAKAFGAFSIRSQEFITRLASSGIQGAAVGRSRGGRSQKDSDEEPVRSREK
                     KGNPRKLPKKLRQRNRIMKKF"
     gene            3263..4507
                     /locus_tag="VLA60_09365"
     CDS             3263..4507
                     /locus_tag="VLA60_09365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HSF09613.1"
                     /translation="MPSTRMISLYSCIGMLFLLGSLPLTGGATETGPFPGEEAQPLIH
                     LIAQSPPSTSIRNIRAHRHKNYTRVVLDLTRSVHPAPQEHQTATTFQLELPNTQLANS
                     AVTKPTAASLPLQLDLSTTSSGSILLSIPIDGWKRYKWYVLKNPARIVLDLYPITETS
                     ALARAPDRSTPGSPVVPPPTPPPPVRKKDLVIVIDPGHGGKDPGALGRKGTREKDVVL
                     QVANHLRDLLAKESSTKVFMTRETDVFIELEDRATFANTHKADLFVSIHINSHSQTAV
                     KGLEIYHFGEASDPRALAVAARENGTPLEKNAAPWQFILADKLNDKKIDDSRELAWTT
                     KNSLVKFLDTFYKIKDHGIKTAPFFVLRMTTMPAILAEIAFISNPTEEKLLQSSTYQK
                     RVAEGIFKGLQSYITPLQTASR"
     gene            4652..5386
                     /gene="truA"
                     /locus_tag="VLA60_09370"
     CDS             4652..5386
                     /gene="truA"
                     /locus_tag="VLA60_09370"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HSF09614.1"
                     /translation="MATIKLTIEYDGTAYVGWQRQPHQPTIQAALETALTRITQQHIS
                     VIAAGRTDAGVHARGQVVSFQSDKPIPIHKWGLAINSALPHDISVLSSEEVPASFHAR
                     YSAKEKLYEYRISSHPARPAIDRNRVWHLPYDLDIPAIRQAMSGLIGSHDFTSFQGPR
                     ASTSDPMCIVSQVSLSSDLMSLTIQIQANRFLKQMVRAIIGTLTEVGRHKRSPDSIQD
                     ILQAKDRRAAGETAPPQGLYLLQVLY"
     gene            5488..6762
                     /locus_tag="VLA60_09375"
     CDS             5488..6762
                     /locus_tag="VLA60_09375"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HSF09615.1"
                     /translation="MNTDFAPEFNQPTFRLAVAQFDQAAHHMNLDSGLLERLKAPQRS
                     LCVSIPVRMDNGKVQVFRGYRVHHDVARGPTKGGIRYHPDVSLGEVAALAMWMTWKTA
                     LAGLPFGGAKGGVAVNPSLLSPAELEGITRRYIAEIFPLLGPDKDIPAPDIGTNQQVM
                     GWVMDTYSQQVGFTVRGVVTGKPLSIGGTLGREEATGRGVVDVTLEVLRHFGLSTSDT
                     TVVIQGFGNVGSHTARILHQEGVKILAISDQMGGLYNQKGLDIPGILSHLATEKASIP
                     SLTQFGEQIINEDLLLLPCHVLIPAAVSEQITVNNASRLQCQYLIEAANGPTTLEADA
                     ILQERGIFVVPDILANAGGVIVSYFEWVQDAQRFSWQESDIHSRLRNIITAAFSRILH
                     HAEEKKLTMRTAALIAGIEEVAQAHQCRGLYP"
     gene            complement(6826..>7207)
                     /locus_tag="VLA60_09380"
     CDS             complement(6826..>7207)
                     /locus_tag="VLA60_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HSF09616.1"
                     /translation="ETGIDVVAYAAQIAAQLGAHIIKVKVPSNHIEQAAAKKVYEAEK
                     IPISAPAERIRHVVQSTFQGRRIVIFSGGAKGEDQKIFDEARGIRDGGGFGSIIGRNS
                     FQRPRPQALEFLSTVMKIYAGELS"
BASE COUNT         1801 a         2049 c         1601 g         1756 t
ORIGIN      
        1 ccgctgtgga tgccaccaaa caggccgtca aaacggactc tttcgacgtg taaatatcgt
       61 acccgccgca taaaagggag ggggacttcc cccttcacca ttcccaacct cgcaagtgcc
      121 gggctcactc cattccgttt gtggtaaaac gaaggaaggc ttgcctcttc ccattgtcca
      181 gacttgagat tcatgcgaga gtagatccta tgacaccctc agagttgaac gcctcccctc
      241 tcacgtatcg acttaagctg ggcctcgtcc tcaatggcat catcattctc gctgaattca
      301 tcgggggcta tgcgatcaat agtcttgggc taatgagcga tgccgggcat aatttgatcg
      361 atcaaggctc tctcttatta gccttgtatg cacatattct ggccacccaa cccgccacgg
      421 aacatcggac gtttggctac caccgcgccg gaaccattgc ggcgtttctc aatagccttc
      481 tgttaatttt cacaggggga attattggag tgtttgctct cgaccggatg ttttcaccgg
      541 ttgacgtacc tggcttttgg gtgatgatga tcgcgtgtat cagcttgatc gccaatcttg
      601 ccgtcgccct gttattgcgt cgtggagccg aagatgacct gaatattcgc ggggcctttc
      661 tgcatatggt gatggatgcc tggatgtctc ttggcgttat catcgccggg ttgggtatcg
      721 ccctgaccgg gttcaccatt ttagatcctc tgatcagtct cctcatagtc atcattatcg
      781 tgaaaggcag ttggccactc tttcgtgagt cgctggatat tttattggaa tcgacccccc
      841 ctcacctcaa aacatcagat attgtggcta ccattgaaaa cgttcctggc gtcagaaaag
      901 ttcatgatct gcatatctgg tcggttgaac cccgcattgt gatgttgacg tgtcacgtgt
      961 tggtagaccc cgaggcggtt ccggataaag atcaactact ccagcccatt caatcaatac
     1021 tctcatcgaa attcggcatt catcacttaa ccattcaatt ggaaaccgaa tgccaggagc
     1081 aagaggacct gcattgcaat cttagttacc tgacgaacgg ctcaaccggc gcagaaccca
     1141 tccctcctca tctgcaccat cattaacccg caagaggctt ccttcgaaac actagcgtct
     1201 tgagtaaaaa tcccacccca cctcctaaca gcgccccaag gccaaccccg accaacacgt
     1261 ctgtcacata atgcgcacct agaaacacac gggacaatcc aatcaggcca aggagtggcc
     1321 acaataccca ccctagcccg ggataaagca tgacaagaaa gctaatagcc gtgctggaat
     1381 tcaacgcgtg attggacggc atactaaaag cccccccaca cccgactaat tcatgaatgt
     1441 gctcgagaac ctggcaaggc cgcggacgac taatcaagac tttcatctgg ccgcccagga
     1501 aatcactcaa gccgatcagg agccctaaac agggaatcgc gagtctggct tcgccccatt
     1561 tcatccaccc ccaatacccc acgagcaaga tgccgggaat caccagattg ctttcctgcg
     1621 acacctgaag catgaaccaa tccaatcccg caaactgacc ggcccaccca ttaatggacg
     1681 caaaccataa ttcatcgaaa ttcatcgttg tcccgtacct attccggact tgtcttcaaa
     1741 ggacgcaaca tcctgcaaga aattggacgg gtatgataag gtacagtttt tataacaaga
     1801 aaggccttct tcatgctgat cgatacccat gttcatttgg acgatccccg ttatgatcca
     1861 gatcgagaca ctatatttca acgtgcccag gaagccggcg ttgaaaagtt tgtcactatt
     1921 ggatgcgatc tttctacaag tcatgcagcc gtgcaattag ccacaagccg acccgatgtg
     1981 tacgccacaa tcggagtcca tccccatgaa gtcaaactca ttgagcccaa ctggtatgcg
     2041 gaattcaggc aattggccca acacccaaag gtagtcgcct tcggcgaaat cgggcttgat
     2101 tatcattacg atcactctcc tcgagaaacc caacgccagc gatttcgtga acaaattgaa
     2161 ctggcgcaat cactcaccct tccactggtg attcatacac gtgaagcgca agaagatacg
     2221 atggcaatcc tgcaagaagc acatgccgaa aaggtgggcg gcgtcttcca ttgtttttcg
     2281 gaggatctgg catttgccaa acgtgctttg gacctgggct ttcatctctc attttcagga
     2341 atcattacct ttcgcaatgc ctctcagtta catgaagtga ttcgcacggt ccccgatgac
     2401 cggttgctca ttgaaaccga tgccccctat ctgactcccg tgccgtttcg aggaaaacgt
     2461 aacgaatcgt cctatgtcac ctacgtggca gaacagattg cgaaaattaa atatggtgac
     2521 tctgaaacag gattggctcg tatagccgaa ctgacaacca ataatgcctg tcggctcttt
     2581 aaaatttctt catgatccga ttgcgttgcc gcaacttctt gggaagtttc cggggattgc
     2641 cctttttctc tcttgatcgg accggctcct catcactatc cttttgggag cgtccccctc
     2701 gggatcgacc caccgctgct ccttgaatac cggaagaagc caggcgtgtg ataaattctt
     2761 gagacctgat cgaaaacgcc ccaaaagctt ttgcaacagc gatcacttca agatccgaag
     2821 agacgactgc ggcccctttc ccatggctcc ggataaaatc ctgaatcacc ccgtccgcct
     2881 tttcaccctg gcccgtgtaa atgacggtca ccccattccg gtggatcacc tgccggctcg
     2941 ctcccgattg ttgccaggca tcaaaaacta aggtgatcag attgtggagt ttgtgcccat
     3001 agaggcctac ccgcacaata aactcctctc ggcgatgctc accctgttca acgacccgat
     3061 gaggcgggag ccccattgcg cccaacacat tatatccatc gataatcaaa tgttttggtg
     3121 ccactgtggt tccttcatga tgcgatgcat gatctacccc tagtgcttga gtctacccgc
     3181 aacaggacct gcttcgaaag cgcttcttcc cttgacaaga aggcgatctt ctgcgaaaaa
     3241 agaaaggaac ggtttactac ctatgccttc cactcgaatg atatcccttt attcctgcat
     3301 cggtatgttg ttcctgttgg gatctcttcc cctgactggg ggagcaacag aaactggacc
     3361 gttcccgggg gaagaagctc aaccactgat tcatcttatt gcacaatcac caccgtcaac
     3421 ctccatccgc aatattcgtg ctcatcgcca taaaaactat acccgtgttg tattagacct
     3481 cactcgctct gttcatccag cgccccaaga gcatcaaacg gccacaacat ttcaattgga
     3541 acttcccaac acccaattgg ccaattctgc cgttaccaaa ccaacagcag cctcacttcc
     3601 tttacaattg gacctttcca ccacttcttc cggatccatt cttctcagca tacccattga
     3661 cgggtggaaa cgctataagt ggtatgtcct caaaaatcct gctcgcatcg tgcttgactt
     3721 gtaccccata accgagacgt cggctctcgc ccgtgcgccg gacaggagca caccaggttc
     3781 tccagtggtt cctcctccaa ctccgccacc acctgtcaga aaaaaagatc tggtgatcgt
     3841 cattgatccg ggacacggag ggaaagaccc cggggctttg ggacgcaagg gcacaagaga
     3901 aaaggatgtg gtcttacaag ttgcgaatca tttgcgagac ctgctcgcca aggaaagctc
     3961 cacgaaagtt ttcatgactc gagaaacgga tgtcttcatc gaattggaag accgggcaac
     4021 attcgccaat acgcacaagg cggatctttt cgtctcgatt cacattaact cccattccca
     4081 gactgccgtg aaaggtttag aaatctatca ttttggtgaa gccagtgacc cacgcgcact
     4141 tgctgtcgct gcacgggaaa atggcactcc tcttgaaaaa aatgccgcac cctggcaatt
     4201 tatccttgcc gacaagctta acgataaaaa aattgacgat tcccgtgagt tagcatggac
     4261 aaccaaaaat tccctggtga aatttttgga taccttttat aaaattaagg atcatgggat
     4321 caaaacagct ccattttttg tgctgcgaat gaccaccatg cctgccatct tggctgagat
     4381 tgcctttatc tccaacccaa cggaagaaaa gcttttacaa agttctacct atcaaaagcg
     4441 agtggccgag ggcattttta agggacttca atcctacatt acccccttac aaaccgcatc
     4501 ccgatagccg ggattgtctc cgccttcttc aattggcttc ctctggcctc acgctcccat
     4561 aacaatcggc acactgtaaa ctcatgattt ccacgcccat agccttcctg acgccaaagc
     4621 ccgtttcgtc atcctccttc cccttcgaat catggccacc attaaactaa caatcgaata
     4681 tgacggcacg gcttacgttg gatggcaacg acaaccccat cagccaacca tacaagccgc
     4741 actagaaaca gcgctcacgc gcatcaccca acaacacatt tctgtcatcg ccgcgggacg
     4801 aaccgacgct ggcgtccatg cccgtggaca agtcgtcagc tttcaatccg acaaacccat
     4861 tccaattcat aagtggggac tggcaattaa tagcgccctt ccgcacgaca tttctgtcct
     4921 gtcgagtgaa gaggtgcctg catcgtttca tgctcgctat agtgcaaagg aaaaattgta
     4981 cgaatatcga atctccagtc atcctgcccg cccggctatc gatcggaatc gtgtctggca
     5041 cctaccctat gacctggata tcccggccat ccgacaggcc atgtctggct tgataggttc
     5101 tcatgacttc acctcgtttc aaggcccacg tgccagtacc tccgatccca tgtgcatcgt
     5161 gtcccaagtt tccctcagct cggatctcat gtccctgacc attcagattc aggccaatcg
     5221 attcctgaag caaatggttc gggcaattat tggcaccctg accgaagtcg gacgacacaa
     5281 aagatcaccg gattccattc aagatattct tcaggctaaa gaccgacggg ctgcaggaga
     5341 aaccgctcct ccccaagggc tttatctcct tcaggtgttg tactaaaccc tcataaattt
     5401 tttccaggga aagaaactac ttatgctgag attcctgata tacttaagaa acaggacttc
     5461 aaatttccat ctcactggaa accttgtatg aacactgact ttgctccaga gtttaatcaa
     5521 ccaacatttc gcttggcggt ggcgcaattt gatcaggccg cccatcatat gaatctggat
     5581 tcaggattac ttgagcgact caaagcccct caacggtctc tctgcgtcag catccctgtc
     5641 cggatggata acggaaaggt tcaagtcttc cgtggctatc gagtgcatca tgatgtagcc
     5701 cgcggcccca caaaaggcgg catacgctat catcccgatg tcagccttgg ggaagtcgcg
     5761 gccttggcca tgtggatgac ctggaaaaca gcgttagcgg ggttgccgtt tggtggagcc
     5821 aaaggtggag tcgccgtcaa cccctcactt ctttcaccag ctgagttgga aggcatcacc
     5881 cgacgctaca tcgcagaaat atttccgtta ttgggaccgg acaaagatat ccccgctccg
     5941 gatatcggca ccaatcagca agtcatggga tgggtgatgg atacctacag tcagcaggtg
     6001 ggatttaccg tccgtggggt ggtcacgggc aaaccccttt caattggagg caccctgggc
     6061 agggaagaag caaccggccg tggtgtggtg gatgtgacgt tggaagtcct ccgtcatttt
     6121 ggtctgtcca cttcagacac gaccgtagtc attcaagggt ttgggaatgt cgggtcccat
     6181 actgcacgaa ttcttcatca ggaaggcgta aaaatactgg cgatcagcga tcagatgggc
     6241 gggttgtata atcaaaaagg tttggatatt ccaggtattc tctctcactt ggccaccgag
     6301 aaagcgtcaa ttccttctct cacgcaattt ggtgaacaga ttattaacga agatttactc
     6361 ctgctaccct gccatgtgct catcccagct gccgtatctg aacaaatcac cgtcaataac
     6421 gcctcgcgcc ttcaatgcca gtatctaatc gaggcagcta acggcccaac aactctcgaa
     6481 gcggatgcca tattgcagga acgaggaatt tttgtggtcc cggatattct cgccaatgcc
     6541 ggtggagtca tcgtttcata ctttgaatgg gtgcaggacg cccaacgttt ttcgtggcag
     6601 gaatcagata tccacagccg actcaggaat atcatcaccg cggcttttag ccgcatactc
     6661 catcatgcgg aggagaaaaa actaacaatg agaaccgccg cattgatcgc aggaattgag
     6721 gaagtggctc aagcccacca atgccggggg ttatacccct gattccacgg ccttcgttga
     6781 cctctctagt tggcgcagat atttttaaac ggactgctgg aatctttatg aaagttctcc
     6841 ggcatagatc ttcatgacgg ttgagagaaa ttcaagagcc tgcggtctcg gccgttgaaa
     6901 ggaatttctc ccgataatgg atccaaaccc tccaccatcc cgtattcctc tggcttcatc
     6961 aaaaattttc tgatcctccc ctttggcccc gcccgaaaag atgacaatgc gccgcccctg
     7021 aaaggtactc tgcacgacat ggcggatacg ctccgctggc gcgctaatag gaatcttttc
     7081 ggcctcgtac accttttttg ccgccgcctg ctcaatatga tttgaaggca ccttcacttt
     7141 gataatgtga gccccgagtt gggctgcaat ttgtgcggcg taggcgacga catcgattcc
     7201 ggtttcc
//
LOCUS       DATIAL010000298        13851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_593755, whole genome shotgun sequence.
ACCESSION   DATIAL010000298 DATIAL010000000
VERSION     DATIAL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13851
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593755"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VLA60_09385"
     CDS             complement(<1..983)
                     /locus_tag="VLA60_09385"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HSF09617.1"
                     /translation="MATESFEPSPPGLLPRQDFSHLIRILQNLGYRILGPVLSNGAIL
                     WGEITRGEDLPIGWKDQQRPGSYRLAPDSSPRFFNIVHGPQSLKPLVFTPRETLLVLD
                     RNGKTFSAKETVPEIPRTAVLGVRACDLAGLGIQDQIFLNGSYPDPYYQQRRNNLLLI
                     AVNCTRALETCFCASMGTGPKAQEGYDLSLTEADEDFLIQAGSPTGLEVLEHLACGVA
                     SEARIQQDDERIAACAASQTRTLPHASLPHGLYEAHDHPRWDEVAARCLACTNCTMVC
                     PTCFCHGVEETPTLDHQQSEHARVWDSCFTSDHGYIHGKNFRPTTKDRYRM"
     gene            complement(998..2365)
                     /locus_tag="VLA60_09390"
     CDS             complement(998..2365)
                     /locus_tag="VLA60_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09618.1"
                     /translation="MADNHSHPVAGSEEGLHSLLTRLEHQSLQPDFQIQRQLALSHAL
                     QPYFHPLMVPPLVPLPEEEDLARWFVYADYLPTDGHTSLIEQVRDLVTEHVPQEERVW
                     LDSVRHSYMDLLEVQALDQGKQATQTRLQSLGDQQIFDVLPHTRPVPYKVGQVLLTRL
                     LRGLADIRLPGPPLILSSSMGKAVFELTNEMRRDIEIGTGNFALSEWAEFAKSFGYMI
                     MWSVAKVRRGAWTVVDSQIDYQNTQGETFLYAIALYEHHDFRAMADGLRELDQFTPGQ
                     EHAGKPASTASQTDTLTWVWLPPQDPSGDGACPIARLTLTPTQLFAETDSADRLDTVK
                     HQLAATFGFLLHFQGETTAPPQHRTPEVDLLSDSYIAPPTIVSKEEEQQILASWLETV
                     YLEWAERPSPLLKGQSPRHFCSAQQDTKEVAAIIDQMEQHDLGLRRTGQSAYDYNILR
                     GHIGL"
     gene            complement(2383..3345)
                     /locus_tag="VLA60_09395"
     CDS             complement(2383..3345)
                     /locus_tag="VLA60_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF09619.1"
                     /translation="MVKIVTHILVPTDFSPSSTPAFRYAVEWAKVFEAQLTILHVHSL
                     QPGLDIDAGVAQQFLDEQRKVAREELETLLAEARQQVPRASMELLAGLPSECICEVAR
                     EKTCDLIMMGTHGWTGFNRVLFGSVAERVIQRAPCPVLTIPHRESADISAMHPLQILP
                     KQVVLPVEFSDCSMDAYEYAVQIAKWFDVPLTLVHAIEPLSYSLDFTLTHPLQEKTNR
                     DKVEKRLADLTAVLSEQGLSARYELLDKPTVDGILEISAIQQADLIVMGTHGRKGLTR
                     VILGSTAYKLLEQSPYPVLTVKSPKFEGGHHPSLANDKTTSPPA"
     gene            complement(3452..3877)
                     /locus_tag="VLA60_09400"
     CDS             complement(3452..3877)
                     /locus_tag="VLA60_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09620.1"
                     /translation="MHNLRKCLFRRIWVLSIGFFLLGGMSPTSAQPIQEVIVTIEGYT
                     FQTTQMPLQLDTETIIYIENRDSVRHDFGSDMFLNTLTHVDSNGVVTYGKGVEGVYLE
                     PGQEASIRLMLNHSGRFQFQCSIHKEMKGEILLLTVDAV"
     gene            complement(3998..4453)
                     /locus_tag="VLA60_09405"
     CDS             complement(3998..4453)
                     /locus_tag="VLA60_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSF09621.1"
                     /translation="MSAITRWDPFRELDELQGRLSTLFGRAPIRKEGERQEALRVAEW
                     APLVDITEDPKEYLVKAELPEVKKDEIKISVQNDVLVISGERKYEKEEKDKKYHRIER
                     AYGSFSRSFTIPEDADPEKVSAEFKEGVLHVHLPKSDRAKPKSIAVKVS"
     gene            complement(4689..5552)
                     /locus_tag="VLA60_09410"
     CDS             complement(4689..5552)
                     /locus_tag="VLA60_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF09622.1"
                     /translation="MRIVVPVDGSSCSTSSVQTLAHFTPPEELTLVHALHLPDFNYPM
                     ITPDLRTEAQEEIKDQLRKEGEGILDETQKHLPADFSHVQRVHQIGHPVDVIVETARS
                     AQSNLIVLGARGLGPVKELILGSVSHRVLMHAPCSTMIVKTPMTQLKKILLPIEGQED
                     AEAALQFLALQPFRQPVEVEVFAVWPQPQLSWPTTVGQSDRLEAQAIEEAQERMKAVT
                     DRLIRMNFACQAHVGIGDPAYAILEQRHASQSDLIMMGMHGRGGFSRFLMGSVSHSVL
                     HQTPCPVLIVR"
     gene            6025..7392
                     /locus_tag="VLA60_09415"
     CDS             6025..7392
                     /locus_tag="VLA60_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF09623.1"
                     /translation="MINDSPAVLVVDDEPEMRQLLRDILEEEHYRVMVASDGHDALNR
                     MEAEKFPVVVTDLRMKGMDGLGLLDHVLRKHPESNLIMMTAFGTVESAVDAMKQGAFD
                     YLTKPIKSNELSVTVAKAMREALLRQEVKQLRQQVSREFSFDQILGKSKPMKEIFDLI
                     RRVADSQTNILITGESGTGKELVAKAIHFNSQRKAAPFVPVNCAAIPELLLESELFGH
                     VRGAFTDAKSDKPGLFEEAHNGTLFLDEISEMPMMLQAKLLRAVQEREIRRVGATRSL
                     SINVRLVVATNVHLREEVKAKRFREDLYYRLNVIELHLPPLRDRKEDLPILVQGLLQK
                     SVTAQQKHIESVEEPAMARLMDYQWPGNVRELENILERAATLTQGKKICLDDLPSNIR
                     NIQGDGQLIGDAAERLLPLEQLEKSYIRRVLEKMGGNKYQTAHVLGIDRKTLYRKLAE
                     MEEVV"
     gene            7554..9509
                     /locus_tag="VLA60_09420"
     CDS             7554..9509
                     /locus_tag="VLA60_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF09624.1"
                     /translation="MSHDPRPPDLHQEVYLLRQELAELRAVQAMHQEARDGLEAIEQQ
                     LAGIIHSAMDAIIIIDDKQRIVIFNAAAEEIFQCSAQEALGNTLDRFIPLPLRSAHRE
                     HIRRFAETGATSRRMGANMEISGLRGNGETFPLEASISQAERGGKRWLTVILRDISQR
                     KQQEERLSYLGRILEESVNEIYVFDATTLRFTQVNKGARENVGYTMEELCVMTPLDLK
                     PDLPNNQFLELLALLQSGQREQVNFRTSHRRKDRTTYPVEVHLQYLQEEGRRVFLAII
                     MDLTERVATEQELQEAQRTLSTLLKNLPGMVYRCDNDRDWTLEFVSPSCQDLTGYPPE
                     KFVQSREVSYARDVMLQEDRERVWQEVQEALKDRKPFQSSYRIRTVEGAVKWVWEQGC
                     GIFSETGEVVGIEGYVVDITQQRALEDQLRKTERLAELGTLASGMAHEIGTPMNVILG
                     RAELLMRKAQDERTRRGLETIVNQVERITKIMNQLLSFARKRPAVRQAVNLEVVMIDV
                     LDVLQERLGKQHIQVKKTVSPHLPKVLADPDHMNQVLLNLILNACQAMGDGGTLSLAL
                     HPTDDTVELTVQDTGSGIPHEQLAKIFDPFFSTKAVGEGTGLGLTVVHGILQEHQGAI
                     RVSSVPGQGTTFIVSLPIYSEEARREA"
     gene            complement(9610..10479)
                     /locus_tag="VLA60_09425"
     CDS             complement(9610..10479)
                     /locus_tag="VLA60_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HSF09625.1"
                     /translation="MSRLREIRRILWWILILNLAVAAAKWGYGVVTHSLGMQADGLHS
                     TFDGLSNVIGLLGLWLTAPPPDANHPYGHKKFEALTAGSIGGFLVITCVYLLWKAYQS
                     WAFDVHPQVTGTSFVIMIGTMGVNIFVTRWERRKGTELKSDILTADSYHTASDVLTSC
                     SVLAGLLAVKAGYAFVDPLVAVLIAGVIAWTAFIVLKDVLSSLTDAVRLDPGEVHTAV
                     MSIAGVLHCHDIRTRGLNHHVFMDLSVHVDPTLSVDKAHALATRIEEHLIDHFESLED
                     VIVHIEPEGHERG"
     gene            complement(10652..12070)
                     /locus_tag="VLA60_09430"
     CDS             complement(10652..12070)
                     /locus_tag="VLA60_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HSF09626.1"
                     /translation="MRERGLLPDFSRGVIDEIDGISGPATQVTELTRDLTTLPWCSID
                     NDDSRDLDQLTVAEPMPDGAAKVLVAIADVDAIVKKGSAIDDHARHNTTSVYTVAETF
                     PMLPETLSTNLTSLNFESVRLALVIEMILAADGSLQRSDLYGATVRNHAKLAYNSVAA
                     WLQNQGPMPPAIAAVNGLDENLRLQDRMAQKLKALRHFRGALDLETIEARPVFEQDEL
                     KNLESETTNRAKDIIADFMIAANGVTVRYLTANGFPSLRRVLRSPERWQRIVDFAASF
                     GEPLPSEPDAGALEEFLLKRRQADPLGFPDLSLTIIKLIGRGEYVLNLPGETPSGHFG
                     LAVKAYTHSTAPNRRFPDLITQRLIKAVLSNDPVPYRVEELKSLATRCTEKENDAEKV
                     ERRLQKSAAALLLESKIGQQFDAIVTGASNKGTWVRLVPLPVEGKLVSGFSGLDVGDR
                     VQVDLISTDVERGFIDFAKAGP"
     gene            complement(12237..12932)
                     /locus_tag="VLA60_09435"
     CDS             complement(12237..12932)
                     /locus_tag="VLA60_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HSF09627.1"
                     /translation="MRVRHRERHRTERIGWLRATVLGANDGIISTASLLLGVSAAGAT
                     HSEVLVAGMAGLVAGAMSMAAGEYVSVQSQADTEEADLALERTELITDPSGEHKELAG
                     LYVERGLDPPLAKQVAGQLMAHDAIGAHARDELGISENLRARPIQASLASAGSFTLGA
                     AMPLLTAFIVPGASLIPVVSGTSLVFLALLGGLAAHAGGAPKMKGAMRVTLWGALAMG
                     LTAWIGALFGTVA"
     gene            complement(13024..>13851)
                     /locus_tag="VLA60_09440"
     CDS             complement(13024..>13851)
                     /locus_tag="VLA60_09440"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HSF09628.1"
                     /translation="KALYASKKQYKQVLKKHIEDLSDLQRLLYASNRYALLLIFQAMD
                     AAGKDSAIRHIMSGVNPQGCQVFSFAHPSAEELDHDFLWRTTQALPERGRIGIFNRSY
                     YEEVLIVRVHPEILYSQGLPDELLDEQTIWQERYRSIVDLESHLHRNGTRILKFFLHL
                     SEEEQRKRFLQRIDEPEKNWKFSLADIEERKYWDQYMQAYEACLSATSTETAPWYIVP
                     ADDKQNARLIISHMIVETLGSLNMRYPQPTKARLKELQTIRKRLARNARKLKSAKED"
BASE COUNT         3222 a         3422 c         3871 g         3336 t
ORIGIN      
        1 cacatgcgat agcggtcttt ggtggtgggc cggaaatttt tcccgtggat gtagccgtga
       61 tccgacgtga aacacgaatc ccagactcga gcatgttccg attgttggtg gtccagagtg
      121 ggagtttctt ccaccccatg acaaaaacaa gtagggcaaa ccatcgtaca gttggtgcag
      181 gccaggcatc gtgcggcgac ctcgtcccat ctggggtgat cgtgagcttc atataagcca
      241 tggggtaacg aagcatgtgg aagggtcctc gtctggctgg ccgcgcaggc ggctatccgt
      301 tcatcgtcct gctgaatccg ggcttcagac gccacaccgc acgcaaggtg ttccaatact
      361 tccagccctg tggggcttcc ggcttgaatc agaaagtctt catcggcttc ggtcaggctg
      421 agatcgtacc cttcttgggc ttttggtccg gtccccattg atgcgcaaaa gcacgtctcc
      481 agcgccctcg tgcagttgac ggcgatgagg aggagattgt ttcgtcgctg ttggtagtag
      541 ggatcaggat aggaaccatt caggaaaatc tgatcttgaa tgccgagtcc tgcaaggtcg
      601 caggctcgta ctcccagcac ggcggttcgt ggtatctcag gaacggtctc ttttgcagaa
      661 aatgtttttc cgttgcggtc cagcactaac agggtttctc gtggagtaaa caccagtggc
      721 ttgagagatt gtgggccgtg aacgatgttg aaaaaccggg ggctggagtc tggtgccagg
      781 cgataacttc ctggacgttg ctggtccttc cacccgatcg ggaggtcttc tccccgggta
      841 atttcgcccc atagaattgc gccgttggag aggaccggcc caaggatccg atagccaagg
      901 ttctggagaa tgcgaatcag atgagaaaaa tcctggcggg gtaagagtcc tggcggagaa
      961 ggttcaaaag attcggtggc catgattgcc ttataactta cagcccgatg tgaccacgga
     1021 gaatgttgta atcatacgcg gattgtcctg tgcgtcgcag acccagatca tgttgttcca
     1081 tttggtcaat gatggctgca acctcctttg tgtcctgttg cgcggagcag aaatgccgcg
     1141 ggctttgtcc tttcagaagc ggcgaaggcc tttctgccca ttccaaatac acggtctcaa
     1201 gccacgatgc gaggatctgt tgttcttctt ctttggagac aatggtgggg ggagcgatat
     1261 agctgtctga taagagatcc acttcaggag tccgatgttg agggggagcg gtggtctccc
     1321 cctggaagtg caataagaag ccaaaggttg ctgccagttg gtgcttgacg gtatccagcc
     1381 ggtctgcaga atcggtttcg gcaaatagtt gagtgggtgt caaggtgagg cgggcgatgg
     1441 ggcatgctcc gtcacctgat gggtcctgtg gtgggagcca tacccacgtt aaggtatcgg
     1501 tttgtgaagc cgttgacgcg ggcttgccgg cgtgttcctg cccgggagtg aattgatcaa
     1561 gctcacgaag cccgtcagcc attgcacgga agtcatggtg ttcatacaag gcgatggcat
     1621 agagaaaggt ttcgccttgg gtattctgat agtcaatctg tgagtcaacg acggtccagg
     1681 ctcctcgtcg caccttggcg acactccaca tgatcatata tccgaagctc ttggcgaatt
     1741 ctgcccattc tgaaagggca aaattgcccg tgccaatctc gatgtcccgt cgcatctcat
     1801 tggtcaattc aaagaccgct ttgcccatgc tggaggagag gatgagcggg ggaccaggca
     1861 ggcgtatgtc ggccaatccc cgaagtagcc gggtgagcag gacctgccca actttgtagg
     1921 gaaccggtcg cgtatggggg agaacatcaa atatctgttg gtctcccaac gattgcaaac
     1981 gggtttgagt agcctgcttc ccctgatcca gcgcttgcac ctcgagcaga tccatatagg
     2041 aatgtcgcac ggaatccagc cagacccgtt cctcttgcgg gacatgttcc gttactaggt
     2101 ctcgcacttg ctcgatgaga gaagtatggc cgtcagtcgg caaataatcg gcatagacga
     2161 accacctggc tagatcctct tcttccggca aaggaaccag gggtggaacc atgagtggat
     2221 ggaaataagg ctgaagggcg tggcttaatg ccagttgtcg ttgtatctga aagtccggtt
     2281 ggagcgattg atgctccaat cgggttaaca gactgtgaag cccttcttcc gatccggcta
     2341 caggatgaga gtgattgtct gccatcgtca caggttcagg gtttatgctg gaggggatgt
     2401 ggtcttgtca ttggcgagcg agggatgatg tcctccttcg aattttggac ttttaaccgt
     2461 caggacaggg taggggcttt gttcgagtaa cttataggcg gtgctcccca ggatcacgcg
     2521 ggttagtcct tttcggccat gggtccccat gacgataaga tcggcctgct gaatggcact
     2581 aatttccaga atgccatcaa cggttggttt atcgagtaac tcatatctgg cggataagcc
     2641 ctgttcggac aggaccgcag tgagatcagc cagacgcttt tcgaccttat cacggttggt
     2701 tttttcttga agcggatgcg tcagggtgaa atccaagctg taggacaatg gctcaatggc
     2761 atgaaccaag gttagaggca catcaaacca tttggcaatt tggaccgcgt attcgtaggc
     2821 atccatggag caatcggaga attccacggg cagcaccact tgtttgggga gaatttgtaa
     2881 gggatgcatc gctgaaatat cggctgattc cctgtgggga atggtcagaa ccgggcatgg
     2941 ggctcgttga atcacccgtt ccgccacgct cccgaataag actcgattga atcccgtcca
     3001 cccgtgggtt cccatcataa ttaaatcgca tgttttctcc cgggcgacct cgcaaatgca
     3061 ttcagagggg aggcctgcca gcagttccat ggatgccctc ggaacttgtt gccgagcttc
     3121 ggccaggagt gtttccaact cttcccgtgc cacctttcgt tgttcatcca gaaattgttg
     3181 ggcgactccg gcgtcgatat ccagccctgg ttgcagggaa tggacatgaa gaatggtcag
     3241 ttgtgcttca aatacttttg cccactccac cgcatatcga aaggcaggag tggagcttgg
     3301 tgaaaaatcg gtgggaacca ggatatgagt tacgatcttg accataactg tattggcctt
     3361 tcgccttaga gtgaaagcag gagaacacgt tcagaaattt taatagcttg ggcatcagac
     3421 cacaacccat aaaaaattca gaggttgccg attacacggc gtccacggtc aggagtagaa
     3481 tttccccttt catctcctta tggatcgaac attgaaattg gaatcgaccg gagtgattga
     3541 gcatcaagcg gattgatgct tcctggccag gctcaagata gacgccttcc acaccctttc
     3601 cgtaggtgac gacgccgttg ctatcgacat gggtcagagt attcaagaac atgtccgatc
     3661 caaaatcatg gcggaccgaa tccctgtttt cgatgtagat aatggtctct gtgtccagtt
     3721 ggagaggcat ctgggtggtt tgaaacgtgt atccctcaat ggtcacgatc acttcttgta
     3781 tgggctgcgc gcttgtcggg gacatcccac ctaagagaaa gaatccaatg gacagaaccc
     3841 aaatcctgcg aaataggcat tttcgaaggt tgtgcatgtg tggattatct cccttgtgtt
     3901 gaaaagactt gtctcactcg atttgctgcc aatggtcgta ttggcagcat agggtatccg
     3961 ggttcccctt tcaattcaga agggggaacc cgcttactca tgaaactttt acggcaatac
     4021 tttttggctt tgcccggtcg cttttgggta aatggacgtg caacacaccc tccttgaatt
     4081 ccgccgagac tttctcggga tcggcatctt cggggatggt gaaactccgt gaaaagctcc
     4141 cataggctcg ctcgatccga tggtattttt tatccttctc ttccttctca tactttcgtt
     4201 caccggaaat gactaacaca tcgttttgta cactgatttt aatttcatcc tttttaacct
     4261 cgggaagctc ggccttgacc aaatattctt tggggtcttc tgtaatatct accagaggag
     4321 cccattcggc gacgcgcaat gcttcttgcc gttccccctc ttttctgatt ggtgcccggc
     4381 cgaacaaagt tgaaagccga ccctgtaact catccaattc tcgaaacgga tcccaacgtg
     4441 tgatggcgct catcgcatat cctccttttt accctagaga gaaaatattg aacattcctt
     4501 aacctgctat caagcaatgg atatgccaac agtttagaag gaaaggcggc tgagatttaa
     4561 ggaaaaagct ttgggaaaaa ctcgttgctc tccatccttc gtggcaaagc gcctcattca
     4621 aagcgaccag gtctgtagca gatgtcatca acaggaaaaa ggaatctggt cctcctgctc
     4681 ctgagagctt accggacaat caataccggg catggggtct gatgtaagac ggaatgcgaa
     4741 acgctcccca tcaaaaaccg ggagaaccct ccccggccat gcatacccat catgatcagg
     4801 tcggattggg aggcgtggcg ttgttcgagg atggcatagg cgggatcgcc aattccgaca
     4861 tgcgcttggc aggcgaagtt catccggata agacggtcag tgacggcttt catgcgttcc
     4921 tgggcctctt ctatggcttg agcttccagt cggtccgatt gtccgactgt ggtgggccat
     4981 gacagttggg gttgtggcca gacggcaaac acctcgacct caaccggttg tcgaaaaggt
     5041 tggagagcta aaaactgtaa cgccgcctct gcgtcttctt gaccctcaat ggggagaaga
     5101 atctttttta actgagtcat gggggttttg acgatcatgg ttgaacacgg cgcatgcatc
     5161 aggacccgat gagacacgct cccgagaatc agttctttga ctgggccaag acctctggct
     5221 cccaacacga tcaggttgga ttgtgcggat cgtgccgttt ccacaatcac gtcaaccgga
     5281 tgtccaattt ggtggacccg ctgcacatgg gagaaatcgg caggcaggtg cttctgcgtc
     5341 tcatcgagaa ttccctcccc ttcttttctc aattggtctt tgatttcttc ctgggcctcg
     5401 gttcggagat ccggcgtgat catcgggtag ttgaaatcgg gaaggtggag agcatgaacc
     5461 agcgtcaatt cttcaggcgg cgtaaaatgt gcaagagtct gaacagaaga ggtggaacag
     5521 gatgaaccat caactggaac cacaattctc atgggacctc ccttttcaat gttgttctcc
     5581 ttgccctacc atagttgaaa gacagcaaga ggaatgccac cgtgatacca cctaatcttc
     5641 atggaagtgt gcgtttctgt tattttccag ggtgcagcct gataacgaga gaagggaatc
     5701 gtggtgatga gcaaccacag gaatgggctc tcccttctga caaatttttc ccagggtagc
     5761 aagcagtggg gcacctccga ttgaccaatg tggcaaccta gcatggcttg agttggacca
     5821 ggtgacgcaa attgctacag taggaatatt tctgattggt gaaaaaggct acaaatgttg
     5881 attatttatg ttcgcctctc gtcgctaatc cgggagagga tggggagtca ccgaagggaa
     5941 atgtatctta accggggtgg gctgaggctg ttatttttga taaatcgtgg cctcaggtta
     6001 ctgagttaaa caaggaatat ggcaatgatc aacgattctc cagctgtctt agtcgtcgat
     6061 gatgaacccg aaatgaggca attactgcgg gatattctcg aggaggagca ctatcgagtt
     6121 atggtggctt cagatggtca cgacgcgctt aatcggatgg aagcggagaa gtttcccgtg
     6181 gtcgtcacgg atttacggat gaaaggcatg gacgggctgg gattgttgga tcatgtcctt
     6241 cgaaagcatc cggagtctaa tctcatcatg atgacggcat ttggcaccgt tgagtcggcc
     6301 gttgatgcga tgaagcaagg tgcgttcgac tatctcacca aacccatcaa gagcaatgaa
     6361 ttatcggtga cggtggcgaa agccatgcga gaggcgcttc ttcgccagga ggtcaagcaa
     6421 ttacggcaac aggtcagccg ggaattttcc tttgaccaga ttctcggaaa aagtaaaccg
     6481 atgaaagaaa tttttgatct gatccggcga gtggctgatt cacagacgaa tattttaatc
     6541 accggagaaa gtgggacggg aaaagagctt gtggccaaag ccattcattt taatagccaa
     6601 cgtaaggccg cgccttttgt tccggtgaac tgtgcggcca ttcctgagct gttgttggaa
     6661 agcgaactgt ttggccatgt gcgcggcgcc tttacggatg cgaagtcgga taaacccggt
     6721 ttatttgaag aggctcacaa tggcacgttg tttttggatg aaatcagtga aatgcccatg
     6781 atgcttcaag ccaagttgct tcgtgccgtg caggagcggg aaatccggcg ggtaggggcc
     6841 actcgctccc tgtcgatcaa tgtccggttg gtggtcgcca cgaacgtgca tttacgtgag
     6901 gaagtgaagg ccaagcggtt tcgggaggat ttgtattatc ggttgaatgt gattgaactg
     6961 catttacctc ctcttcggga tcgcaaggag gatctgccta ttctggttca ggggttgctt
     7021 caaaaaagtg tcactgcgca gcaaaaacac attgaaagtg tcgaagaacc ggccatggcc
     7081 aggctgatgg attatcagtg gccgggtaat gtgagagagt tagaaaatat cctcgagcgt
     7141 gcggcgacac tgacgcaggg aaaaaagatt tgtttggatg atcttccctc caatattcgg
     7201 aacatccaag gagatggaca gcttatcggt gatgctgcag agcggttact tccccttgag
     7261 caattagaaa agtcttacat ccggcgggtg ctggaaaaaa tgggtgggaa taaatatcaa
     7321 accgcgcatg tgctcggcat tgatcgaaag acgttatatc gcaagctggc tgaaatggag
     7381 gaagtcgtat gagcgcacca agcctcagga tgatcgcgct gaatcatagt gcgaccggag
     7441 ggctccctca cgctccgggt tcagccctgc gagggatgtt ttattggtcc ttctctcatc
     7501 catccattat ttcttagtct cctgtttgtc agaaacaccc tcccggtcaa tcgatgagtc
     7561 acgatccacg acccccagac cttcatcagg aagtttatct gctccgtcag gaattggcag
     7621 aattgcgagc cgtccaggcg atgcatcagg aagccaggga cgggttggag gcgattgaac
     7681 agcaactggc agggatcatc cattcggcca tggatgccat tatcatcatc gatgacaaac
     7741 aacggattgt catctttaat gcggccgctg aggaaatttt tcagtgttcg gctcaagagg
     7801 cactggggaa taccctcgac cgatttattc cccttccgct ccggtccgcc catcgtgaac
     7861 acatccgccg atttgctgaa actggagcaa cttcccgacg gatgggggcc aacatggaaa
     7921 tttccggact gcgagggaat ggtgagacct ttcctttgga agcctcaatt tctcaagccg
     7981 aacgaggggg gaagcgatgg ttaacggtga ttcttcggga tatttcccag cgaaagcaac
     8041 aagaagagcg gttgtcttac ctgggaagaa ttcttgagga gtctgttaat gagatttatg
     8101 tgtttgatgc cacgactctg cgatttactc aagtcaacaa aggcgcgagg gaaaatgtcg
     8161 gatacaccat ggaagaattg tgcgttatga caccgctgga tttgaaaccg gatcttccga
     8221 acaatcagtt tctggagttg ttggcacttc ttcagtccgg tcaacgggaa caagtgaatt
     8281 ttcggacatc ccatcggaga aaagatcgaa cgacgtatcc ggttgaagtc catcttcaat
     8341 atttacagga ggaggggcgt cgggtcttcc tcgcgattat catggatttg acggaacggg
     8401 tggcgaccga acaggaattg caggaggccc aacgaacctt atcgacgctt ctgaagaatc
     8461 tgccgggcat ggtctatcgc tgcgacaatg accgggattg gaccttggag tttgttagcc
     8521 cgagttgtca agacctgacg ggatatccac ctgaaaaatt tgtccagtct cgagaggtgt
     8581 cgtatgcccg tgatgtgatg cttcaagaag atcgtgagcg ggtctggcag gaggtgcagg
     8641 aggcactcaa ggaccgtaag ccgtttcagt ccagctatcg gattcgaaca gtggagggag
     8701 ccgtcaaatg ggtgtgggaa cagggatgtg ggatcttttc agaaacaggc gaggttgtcg
     8761 ggattgaagg gtacgtggtg gatattaccc aacaacgcgc attggaggat cagctgcgaa
     8821 aaacggaacg attggccgaa ttaggcacgt tggcgtctgg catggcccat gaaatcggga
     8881 caccaatgaa tgtgatatta gggagagcgg aattgttaat gcgtaaagct caggatgagc
     8941 gtaccaggcg gggattggaa acaattgtga accaggtgga acgcattacc aaaattatga
     9001 atcagttatt aagctttgcc cggaaacgtc cggctgttcg gcaggctgtt aacttagagg
     9061 tcgtgatgat cgatgttctg gatgtgctgc aggagaggtt gggtaaacag catattcagg
     9121 tgaaaaaaac agtgagcccc cacttgccaa aggtattggc ggatccggat cacatgaatc
     9181 aggtcttgtt aaatctgatt ttgaatgcct gccaggcgat gggcgatgga ggcactctga
     9241 gcttggctct tcacccgacc gatgacacgg ttgaactcac tgtgcaagat accgggagtg
     9301 ggattcctca tgagcaactc gcaaagattt ttgatccgtt tttttccacc aaggccgtgg
     9361 gggaggggac cggcttaggg ctcaccgtgg tccatggcat tcttcaggaa catcaaggcg
     9421 ccatccgtgt gtcgagtgtc ccgggccaag ggacaacatt tattgtttca ctgcccattt
     9481 attctgaaga agcaaggcgt gaggcgtagg ttgaagagga gtcgaagatt gaatctgctg
     9541 aatgcttttt ccccttttct cccgacccct agcttctcac tccgcatgat ttacccttca
     9601 gggttcccat taccctcgtt cgtgtccttc cggttcgata tggacgatca cgtcttccag
     9661 cgattcaaaa tgatcaatga gatgttcttc aatccgggtc gcgagtgcat gggctttatc
     9721 gaccgatagg gttggatcaa catgaaccga gagatccatg aagacatggt ggttcagccc
     9781 gcgtgttctg atatcgtgac aatgcaggac gccggctatc gacatcacag ccgtgtgaac
     9841 ttcaccaggg tccaatcgaa ctgcgtccgt tagagaagac aggacgtctt tgagcacgat
     9901 gaaggccgtc caggcgatca cgccggcaat caggaccgcc accaaaggat caacgaaggc
     9961 atacccggct ttcacagcta agagtcctgc cagaacagaa catgatgtca ggacatcact
    10021 cgccgtgtga taggagtcgg ctgtgagaat gtcgcttttg agttcggtgc ctttccgccg
    10081 ctcccatcgg gtcacgaaaa tattgacccc catggttcca atcatgataa cgaaactggt
    10141 gccggtcacc tgagggtgca catcaaaggc ccatgactga taggctttcc aaagaagata
    10201 gacgcaggtt atgaccagga agcctccgat ggatccggcg gtcaaggctt caaatttttt
    10261 atgaccatag ggatgattgg catcgggagg aggggcggtc agccagagcc cgagcaaacc
    10321 aatcacattc gagagaccat cgaaggttga gtgaaggcca tcagcctgca tacccaatga
    10381 atgcgtcacc acgccatagc cccactttgc ggcagcgaca gcgagattta atatgaggat
    10441 ccaccacaga atccggcgaa tttctcgcag tcggctcatg gatagcgtac aacgatcatg
    10501 ggggcgaaaa gcggatttgt tgcccccgtg aaaacgtgga tatcatcggc tgcataccgt
    10561 cctcactata tcaagaagtt tgctggtgat cgagaatcga agagggaatc ctcttaggtg
    10621 aagaggtggt ttgcggatgg gggagaaacc gttatggtcc agccttggca aagtcgatga
    10681 agcctcgctc gacatccgtg ctgatgagat ctacctgcac gcgatcgccc acgtcgaggc
    10741 cgctaaagcc ggacaccagt ttcccttcga ccggcaaagg caccagacga acccaggttc
    10801 ccttgttgga ggcgccagtg acaatggcgt cgaactgttg tccgatcttc gattccagta
    10861 acaacgcggc ggccgatttc tggagccggc gctccacctt ctccgcatcg ttttcctttt
    10921 cggtgcaacg ggtggcgaga gacttgagtt cctctacccg gtaaggaacc ggatcgttcg
    10981 ataaaacggc cttgatcagt cgctgtgtga tcaaatcggg gaaacgccgg ttgggggccg
    11041 ttgaatgggt ataggccttc actgccagtc caaagtgccc tgaaggagtc tctcccggca
    11101 ggttgagtac atattcgccc ctcccgatga gtttgataat ggtgagtgac agatcgggaa
    11161 atcctaacgg gtcggcttgt cttcgtttga gcaggaactc ttccagagca ccggcatcgg
    11221 gttcagacgg gagaggctca ccaaacgatg cggcgaagtc tacaatcctc tgccaccgtt
    11281 ccggtgaacg caagacccgg cgaagggacg gaaaaccgtt ggccgtgagg taacggacgg
    11341 tcacgccgtt ggccgcgatc atgaagtccg cgataatgtc cttagccctg tttgtggttt
    11401 cgctttccaa gttcttcaat tcatcctgtt caaagaccgg ccgcgcttct atggtttcga
    11461 gatcgagggc accccgaaag tgccggaggg ctttcagctt ttgagccatg cggtcctgga
    11521 gccgaaggtt ctcatcgaga ccgttgactg cggcgatcgc cggcggcata ggaccctgat
    11581 tctgcagcca ggccgcaacg ctgttgtacg ccagcttggc atgattgcgc accgtcgctc
    11641 catagaggtc cgaacgctga agcgaaccat cggcggcgag aatcatttca ataacgagag
    11701 ccagacggac cgattcaaaa ttgagagagg tcagattcgt ggagagtgtc tcgggcagca
    11761 tcggaaacgt ttcggccacg gtatagaccg aggtggtgtt gtgccgcgca tgatcgtcaa
    11821 tggctgagcc cttcttgacg atcgcgtcaa catcggcaat agccactagg accttagcgg
    11881 ctccgtccgg catgggttcg gccaccgtca gctggtccag atcgcgggaa tcatcgttgt
    11941 cgatggagca ccagggaagg gttgtgagat cacgcgtcag ttctgttact tgtgtcgcag
    12001 gtccggagat tccgtcaatc tcgtcgatga ccccgcggga aaaatcggga aggagtcctc
    12061 tttcacgcat cgcctggcgg gcaatgcgtt gcaagatcgc gcggtgtcgc ctgtcgtctg
    12121 aatccgtcat catggctcct ttccggaata ctctgttaag cgttcctgcc tatacacgat
    12181 acgcaacgct taaaaagttc aataacgttc tgccgtttcg tttccggttg gtcctgttac
    12241 gccactgttc caaaaagcgc tccgatccat gcggtcagcc ccatagccag tgcaccccac
    12301 agggtgacgc gcatggcgcc tttcattttg ggggcaccac ccgcgtgggc cgccaatcct
    12361 cccaacaacg cgagaaacac gagtgacgtt ccagaaacca cgggaatcag actggcgcct
    12421 gggacaatga acgcagtcaa caaaggcatg gcggctccaa gagtaaagct gcccgccgac
    12481 gccagggaag cctgaatcgg acgcgcacgg agattttctg aaattccgag ttcgtcgcgt
    12541 gcatgggccc ctatggcgtc atgagccatg agctgaccgg cgacctgttt cgcaagcgga
    12601 ggatcaaggc ctcgttccac atacagcccc gcgagttcct tgtgttcccc tgaagggtcg
    12661 gtaatgagtt ccgtgcgctc aagggcaagg tcggcctctt cggtgtctgc ctgtgattgg
    12721 acagatacat actcaccggc agccatcgac atcgctccgg caaccagacc ggccattcct
    12781 gcgaccaata cctcgctgtg agtcgcaccc gccgccgaga cgccgagtag caagctagca
    12841 gttgaaatga ttccgtcgtt ggcccccaat acggtcgccc gcagccagcc gatgcgctca
    12901 gtacgatgtc gttccctgtg tcttacacgc atgcctcatt ctttcttaaa gggcatctac
    12961 tcaacaaggc aacctccctg cctatcagta cattcactgc acggaaagag aagccgggtg
    13021 cgtttagtct tccttggcac tcttaagctt cctggcgttc ctcgccaggc gtttgcggat
    13081 agtctgcaat tccttcaggc gcgccttggt tggctgaggg taacgcatgt tgaggcttcc
    13141 cagtgtttcg acaatcatat gcgaaatgat gagcctggcg ttttgtttgt catcggctgg
    13201 cacgatatac cagggagcgg tttctgtgct tgtggcactc aaacaggcct cataggcttg
    13261 catgtactga tcccaatact ttctttcctc aatgtcggcg aggctgaatt tccagttctt
    13321 ctccggttca tcaatgcgtt gaaggaaccg cttccgctgt tcttcctctg acaggtgaag
    13381 gaaaaatttc aagatccgcg tgccattacg gtggagatgg ctctccaggt ccacgatgga
    13441 acggtatcgc tcctgccaaa tggtctgttc atcaagcaat tcgtccggca gcccctggct
    13501 gtacagaatt tccggatgga ctcgaacgat cagcacttcc tcgtaatagg aacgattgaa
    13561 aatgccgatc cgcccgcgtt caggcagagc ctgtgtggtg cgccatagaa aatcatggtc
    13621 cagttcctcg gcgctgggat gggcgaagct gaacacctgg cacccctggg gattgacacc
    13681 ggacatgata tgccggatag cgctgtcctt gccggcggcg tccatggcct gaaatataag
    13741 caacaacgca taacggttcg acgcatagag gagacgttgc aggtcgctga ggtcttcgat
    13801 atgttttttg agaacctgct tgtactgttt cttcgatgcg tagagcgcct t
//
LOCUS       DATIAL010000299         4060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1346284, whole genome shotgun sequence.
ACCESSION   DATIAL010000299 DATIAL010000000
VERSION     DATIAL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346284"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VLA60_09445"
     CDS             <1..204
                     /locus_tag="VLA60_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09629.1"
                     /translation="GRDGEGNRQIHILTIIIPVCPVERRGVFIDLKPSHDWMHSDMTG
                     AEGYLHPLCDFQRVGANGDDVKI"
     gene            complement(487..1845)
                     /locus_tag="VLA60_09450"
     CDS             complement(487..1845)
                     /locus_tag="VLA60_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF09630.1"
                     /translation="MKQETRILVVDDEINIRNALVTILEKAGYLAQGADSAESALALM
                     KEAPSDLLITDVRMPGIGGMALVPRIKALWPATEIIVITAYGSIETAVEAMRLGAYDF
                     LTKPIDRERFSIVVEKALDRHRLAAENQNLRHQLETRNRLEEMIGESEAMRRIHHVIG
                     MVAKNDVTILIGGESGTGKELVARAIHQQSQRSNGPFITMNCGALPESLFESELFGYE
                     KGAFSGAVGTKPGRFEMADEGTLFLDELGELPLKAQVDFLRVLETGEFRRLGGKTLIT
                     VNTRIIAATNRNLPQLVETGLFREDLFYRINVVPIHLPPLRERREDIPVLLDYFLKEF
                     AQKHLIPLKTLSLASLRFLVQYPWPGNIRQLRNVIERLVVTVKEHTIQPDHLPDEIRT
                     SVNPGSRMVISLGTPLEDIEREVIRRTLVEVTHHREQAAKLLGISVRSLQYKIKEYGI
                     EL"
     gene            complement(1842..3239)
                     /locus_tag="VLA60_09455"
     CDS             complement(1842..3239)
                     /locus_tag="VLA60_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF09631.1"
                     /translation="MFRPRPETPLFHNLLIRQIVPILIIVGVVVGVLLIFSLEHQSMV
                     VVGLTEGKTVSSELNAAVGQSRRDLIAITLLLFIVGGVGISAVVTYLHYANAKQNLEE
                     VKGLARHILESIPTGVLTVSEDGVITAANPTAEAILERPAISLLGKHYGVVFGEGHPI
                     QVLLAQILHHGGSMHSKEITFDSQNGEVRTIRISSVDLTGDNGKPAGMIFQLHDVTEW
                     LALEKQVNEAQRLAALHTLSAGVAHELRNPLSALDLNLHLLEEELRETTDLSSRANDY
                     FKVLNTELSRLSAILDNIMRYAQSRPSQFQEVNIQSLVSHVTQLLHHEAEDRNQQLQV
                     SVPTNLPDVQGDETQIAQALINIVINAFQAMSAGGVCRIIVQERERQERRWVEIAVHD
                     TGQGIDPQSLVHIFDPFYTTKETGTGLGLAVAHRIIQNHGGRIDVTALPGDGTSVTLS
                     LPVCVNAQAIPVEGA"
BASE COUNT          994 a          990 c         1023 g         1053 t
ORIGIN      
        1 gggagggatg gtgaagggaa tcgtcagatt catattctga ccatcatcat ccccgtttgt
       61 cccgtggaac gacggggagt cttcatcgat cttaaaccct cacacgattg gatgcacagt
      121 gacatgacgg gggccgaagg ctaccttcat cccttgtgtg atttccagag ggtaggtgcg
      181 aatggcgacg atgtcaaaat ttaatccggc ccttgtgcgg tggggattgc cttcgcctag
      241 gcgtggcaac ctcagattaa aacgtcctct gaaattgcag aagggaaaat ggttttcttc
      301 tgatttgaga ggtacaaccc aaatgagccc aacaagtcag gaggtttgtt gggccatagg
      361 gaattaagtg ctcgagagaa ttcaaacaga aggtcgttta tagggcatgg gtgtcaatct
      421 ttctttagat gcaagtcgaa gcagatccgg tccctacctt tgtcttggat ccgttactta
      481 gagattttac aactcaatcc catattcttt gattttatat tggagcgatc tcacgctaat
      541 cccaaggagt ttcgcagcct gttcacgatg atgagtgact tccacgaggg ttcggcggat
      601 gacttcccgt tcaatgtctt cgagaggggt tccgagggag atcaccatcc tacttccggg
      661 attgacactc gttcgaattt catctggaag atgatcgggc tgaatagtgt gctccttcac
      721 cgtcacgacc aggcgttcaa tgacattccg gagttgacga atattacccg gccaggggta
      781 ttgaacgaga aatctcaggg aagcgagaga tagggttttg agtggaatca ggtgcttctg
      841 ggcaaattcc ttgagaaaat agtcgagaag gaccgggata tcctcacgac gctctcgcaa
      901 gggcggcaga tgaatcggca ccacatttat gcgatagaaa agatcttcac gaaacagtcc
      961 ggtttcgacc aactggggca aattgcggtt ggtggcggca ataatcctgg tattgacggt
     1021 gatcagggtt ttccctccaa gcctccgaaa ttcaccggtt tctaagaccc ggagaaaatc
     1081 cacttgggct tttaagggaa gttcgcctaa ctcatcaaga aagagggttc cctcatcagc
     1141 catctcaaat cgaccgggtt tggttccaac cgctcccgaa aatgcccctt tttcatatcc
     1201 aaataattcg ctttcgaaaa gagactctgg aagggcacca caattcatcg tgataaacgg
     1261 gccattggac cgctggcttt gttgatgaat ggcacgtgcc accaattctt tgcctgtccc
     1321 gctctcccca ccgattaaaa tcgtgacgtc atttttggca accattccaa taacatggtg
     1381 tattcgacgc atcgcttcac tttcgccgat catttcctcg aggcgatttc gtgtctcgag
     1441 ttggtgacgc aaattctgat tttcggctgc aagcctgtgg cgatccaagg ccttttcgac
     1501 cacaatggag aatcgttcac ggtcaatggg cttggtgaga aaatcgtaag ctcccaaccg
     1561 catggcttcc acagccgttt caatagaacc ataggccgtg atgacgatga tttccgtggc
     1621 aggccaaagg gcttttattc ggggaacgag agccatgccc ccgatacctg gcattcttac
     1681 atccgtgatg agaaggtcac tgggcgcctc tttcatcaag gccagcgcgg attcagccga
     1741 gtcggcgccc tgggcgaggt accctgcttt ctccaatatc gtgactaacg cattacgaat
     1801 attgatttcg tcatcgacaa ccagaatgcg agtttcttgt ttcacgcacc ctccacaggg
     1861 atggcctggg catttacaca cacgggtaaa ctcaatgtga ccgaggtgcc atcgccggga
     1921 agtgcggtca cgtcaatgcg gcccccatga ttttgtatta ttcgatgcgc gacggcgagg
     1981 ccaaggccgg tcccggtttc ttttgtcgtg taaaaagggt cgaagatgtg gacaagcgat
     2041 tgtggatcaa tcccttgtcc ggtatcgtga acggcaatct ccacccagcg ccgctcttgt
     2101 cgttcgcgtt cctgaacgat aatgcggcag accccacctg ccgacatggc ctgaaacgca
     2161 ttgatcacga tattaatcag agcctgtgcg atctgcgttt cgtcaccctg aacatccgga
     2221 aggttcgtcg ggaccgaaac ctgaagctgc tggttcctgt cctcggcctc atggtgtaat
     2281 aattgtgtga catgcgacac gagggactga atattaactt cctgaaattg agaaggccgt
     2341 gattgtgcat atcgcataat attatccaaa atagccgaca gccggcttaa ttcggtgttc
     2401 agtactttaa aatagtcatt ggcccgactg gacaagtcgg ttgtctcgcg gagttcttct
     2461 tcaagtaaat gaagattgag atccagggcg ctcagaggat tgcgaagttc atgcgcgacc
     2521 ccggctgaca acgtatgcag cgcagccagt cgttgggctt cgtttacctg tttttcgaga
     2581 gctaaccact ccgtgacatc gtgcagttga aaaatcattc ctgccggttt gccattatca
     2641 cccgtcaaat caacactgct aatccgaatt gttcgcacct ctccattttg ggaatcgaag
     2701 gtaatttcct tggaatgcat ggatccccca tgatgcagga tttgggcaag gagtacctga
     2761 atgggatgcc cttccccaaa caccaccccg taatgcttgc ccaatagaga aatcgcaggg
     2821 cgctcaagaa tggcttcagc ggttggattg gctgcggtta ttaccccatc ttcactgacc
     2881 gtcaatacac cagtcgggat actttccaat atgtgccggg ctagcccttt gacctcttcc
     2941 aggttttgtt tggcgttggc gtaatgcaga taggtgacga cagcggaaat accaacccca
     3001 ccgacgatga agagtagaag ggtgatggca atcaaatcac gccgggattg tccgactgcg
     3061 gcatttaatt ccgaggatac ggtcttgcct tccgtgaggc caaccaccac catactttga
     3121 tgctctagac tgaagatcaa gagcactcca accactacgc ccacaataat aaggatgggc
     3181 acgatttgac gaatgagcag attgtgaaaa agtggtgttt cagggcgagg ccggaacatg
     3241 cttagctcca gtttgtatga aatcttctcg gaaactatcg gaaatactgc ccatttctct
     3301 cctattacct taggcgaaga aagggggatg gtgcaatgtc ccttcattcg ttttaacaac
     3361 tgttcatcaa aagacggcgt tccaactcta tcggtggaca tgttctgtta tccggaaatt
     3421 ttcggctatg ccggggaacc ttttgtgcac acctctcttc ttaaaatgat gtcagtgcgg
     3481 ccatgacgcc ttccatggga acccatccat tctgtctatt acgtatacga ttggcagaaa
     3541 tgtcctcatt ccagcttttt acatcgagag gatactgaga tgagggcatt ttacgattac
     3601 ctgtcgagag gattatggcc acagctctag gggggacctt tggaaataca ctgatgatgt
     3661 tttatcccta acccatacaa gccttgtgca agggtagggg gattcagcat agatcgatgc
     3721 gttatccgga catggcatct ccagacttta ttgttcgtaa ttaaaaattc aaggccatgc
     3781 ccggaaaccc atatgatgca tttccatatc tagaattcta gaattccagc cttgaggcga
     3841 cagggtggta agaaaggttg ccacggtctc tttaagaaat tctgcctgaa cgtgatcagg
     3901 agtcggaggc ggttgaagtc caatcccttt tcctgccttt ccatgcgtcg tctggttggg
     3961 ctcatgaccc cttttggaaa gataagatta gagcaatgga cgtgccaagt ggcatatgag
     4021 gagtcgatcg tcgcttattg aaggagagct gagttttcct
//
LOCUS       DATIAL010000300        12825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_272341, whole genome shotgun sequence.
ACCESSION   DATIAL010000300 DATIAL010000000
VERSION     DATIAL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12825
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272341"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VLA60_09460"
     CDS             <1..354
                     /locus_tag="VLA60_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09632.1"
                     /translation="RRLAPQLDQLCIQADTVVDMCRQRPIPVNRLKAGQYQLKVDTLT
                     MQQIARPSLAFGPLAMAFFRDTFSLESLTLTGMAEEHARAYHVFRTRGEENYRRLEGM
                     FSHDPKGGLYARTIG"
     gene            335..883
                     /locus_tag="VLA60_09465"
     CDS             335..883
                     /locus_tag="VLA60_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09633.1"
                     /translation="MHVQLDEEIWEVGDRVQLGEVLANLSDRAQAKGCLVTELIVGNR
                     KMTDRELVPPTLAKVASSFGSIAAVSKHVETIVQYSEKTGQKFGAQLREQGQKFVDAF
                     RQGDGRYHQLDQWFGQIADYLEWLQVHESMGLKKPEVTHDLSFWIKELLNARENEDGV
                     RIADMLEYEVIPRLSRNITLLL"
     gene            1184..2011
                     /locus_tag="VLA60_09470"
     CDS             1184..2011
                     /locus_tag="VLA60_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HSF09634.1"
                     /translation="MALVVNNNIASLNAQRNLGVSSSQLEGSVARLSSGLRITRAADD
                     AAGLGISETLRAQIRSINQAVRNSNDGISLLQIADGGAEGIGNLLGRLRELAEQSASG
                     ILGSNERSFLDQEFVALRSEIDRISSVTEFNGVKLLSGTGNDSLSIQIGFRSSANDTL
                     SLSLNDLNTNTLNLASTNVSTSGNALDALSNIDSAISAVASARANIGSLQNRIDAAVG
                     NLQVANENITAAESRIRDADIAFESAKFVRNQILVQAGTSILAQANTLPQQALALLQ"
     gene            2079..2477
                     /locus_tag="VLA60_09475"
     CDS             2079..2477
                     /locus_tag="VLA60_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HSF09635.1"
                     /translation="MISTLSDSTFFINISIGNEIRLRDGQKAITGDTHRKSETKQPVY
                     ASDFTKDSTANETLTAEQVQATVARLQERVRQIDPRIEISLEKDINQVIFRVVDKESG
                     DLIRQIPSEQMVDLARLFSGQSGLFVEENI"
     gene            2605..4020
                     /gene="fliD"
                     /locus_tag="VLA60_09480"
     CDS             2605..4020
                     /gene="fliD"
                     /locus_tag="VLA60_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HSF09636.1"
                     /translation="MAISFGGLGNGVDFGPVVDALVQAERLPIDALTQKKLQAQKKQT
                     ELGLLGAKLLSFQSLASSLRNRVSFNKNQVNVASPSAQTPLSATVSSSAAPGTYQVTV
                     NQLASAHQIISKASTAVSQTDADIVGGESGTFQFKVGTGSIQTVNLDATGTLDDLRVA
                     INDLGAGVSASILNTGSEASPQFRLVLSANETGLDQAITIVADDTTLDMATTGVDTFQ
                     AAQDSEVVLGVTDAGAGTTAITINRSTNTLTDVVAGLTINLQAVDATNPVTISVSQDN
                     GAVKEAISDFVSGYNEIVAFVNERTFYNTETKERGIFVGESLARTVLDRVRESVFSQI
                     SGLTTYSGASQIGFETQTADGTIKLNETTLDSALNTNFSAVRDLFVKNTTTGTRGIAD
                     LAVNAIDGLDDIDTGSLTRRQNALTKQVKDFTNQITLKEEALARFEQQQRLKFANLDG
                     LLASLQSQLKQLQNAFPSTNG"
     gene            4036..4428
                     /gene="fliS"
                     /locus_tag="VLA60_09485"
     CDS             4036..4428
                     /gene="fliS"
                     /locus_tag="VLA60_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HSF09637.1"
                     /translation="MMLGAQAYANTQIQTASNIQVIVLLYDGAISSMKLAQEGMATLN
                     YQDKARFLDRALRVIGELSASLNMEDGGGIAKDLRRLYEYIQFELTQANLKNEPRRLQ
                     GPIRCLSLIREAWVDLAVQGTKPQAVGI"
     gene            4442..4756
                     /locus_tag="VLA60_09490"
     CDS             4442..4756
                     /locus_tag="VLA60_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09638.1"
                     /translation="MGKVNCLVEQLTQDAIEAARHGQWEQVIVLYDQRLSQGPQQTLS
                     LNTIQVLMESDQWLIARVKEVQAAINQHLNAIQDQRRKLGVLKRQWGENPTTPVRHLL
                     TI"
     gene            5077..5325
                     /locus_tag="VLA60_09495"
     CDS             5077..5325
                     /locus_tag="VLA60_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09639.1"
                     /translation="MTQQSASSAKSLSVLTENSVQNGKLLHSLSNKLLPIVVFSQLAL
                     RRCEDEQLKRQLEKIHGAAEQARDILVEIRSLHAARNP"
     gene            5411..6016
                     /locus_tag="VLA60_09500"
     CDS             5411..6016
                     /locus_tag="VLA60_09500"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HSF09640.1"
                     /translation="MDIVASEDRRQDCRVDVPVCLWVEIPLAFSYEMIDLDGAEFSQW
                     QWDELAVSPDLVKAMVDEKDLTIRDPLLLQMVTRIDWMLTSILRTLGKDKNLKGAIPE
                     LTTVSLCGSGMKFYSEDSYPLDSFLVLRLILRPFIPIQAVGKIVRVNAKSKGKEQGFE
                     IAVEFTKISSDDREAIIRHTLRSQATMQRFRLKQPVDSVLD"
     gene            6189..7694
                     /locus_tag="VLA60_09505"
     CDS             6189..7694
                     /locus_tag="VLA60_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HSF09641.1"
                     /translation="MSRYSHLNWLGRGLQEMLVSDLAQWPQLEVVSRDALGSVLREQW
                     LQQKGFSSVENTVGLGHLKGVRYLIQGRIFLQQDTLSVDLQIVDVETGVVVASVNAQG
                     VESDIPGLEQDLVTHLIGVFDPSLDGVSRSMVGQSSEDRQPHFRGDPGVDKKGRPVIS
                     FEPNPSSMVSHVDAFLSLEKLTYQRREAYRLAETIWDEGCVVEMGQPLYQVWHFSIDP
                     TRSVPVMAIPLSLFFSSHRISNIFEQVQKSGTDSGGNVDSDGFRTTLDEESGAQSLFI
                     EHFQKPRRVFIRALNEKQEVLAIFSDWGWRTERKFSMRGTQGVSLPMWPNPFVTGLVK
                     FPVDWVEREGQHVTFDSVAVPVPDEHALVVLEPIGEFKGEKSQISRLVDSEDILLHGL
                     ETLIKSNWAPAITEGLPMRGYLPGNKRTAVGVVHVQKGKIRDIQFQSWPEDPFFLESL
                     QDLQLKLLGACPECQDSAQAVPHLSDPVKTFRLQLTLVKDIYALHLGSRSH"
     gene            complement(7760..9175)
                     /locus_tag="VLA60_09510"
     CDS             complement(7760..9175)
                     /locus_tag="VLA60_09510"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HSF09642.1"
                     /translation="MSEAEPLSSPFDGVSLLADPIHGYISITVPYQEPHSTEQTEKDL
                     IDSPWVQRLRYILQLQSAHWVYPGAEHTRFQHSLGTMHLAGRFMARLYPSLCQTVSEV
                     PSCHFLEEFIRITALLHDIGHGPFCHFFDHHFLHQFNLTHEVVGQHIIRDHLGPIIKK
                     ICRSPSGPFGPREQLDPEHIAFLILKDPHKSTKRLPPWVIALQPLLGGIYTPDNCDYV
                     LRDSYMCGVAIGPVDIERLLHYTFFSSQGLTIHQSGLPALQMFLHARLYLYSNVYYHR
                     TTRAIDIHLQEIFQPTMEILCKKNPLQSLPHYLQLTDWHVIETVRSWKHARSMHKRQL
                     GMEWSQIIGRHVKWKTAFSTLLPIIQPASSSFQPQVFLNRMRRLLPTKYRHQDIRVDF
                     AHKDPRPINLLNMGNFQIFVWNPAKGTVEKDSLQKYLQYLPARMITLRVFSQDHSIDE
                     ILAKAAEETLRRHWPTLIQPV"
     gene            complement(9300..9749)
                     /locus_tag="VLA60_09515"
     CDS             complement(9300..9749)
                     /locus_tag="VLA60_09515"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HSF09643.1"
                     /translation="MVTLEAVRQQLTQVLHLRETPHRTALAFALGVFIAFAPHYLFHT
                     ASVVFCAWAFRLNFLALFLGSLINNPWTLIPILAASLYTGMLLMGVSSPVTIEWGQMR
                     VDNIFDMLSPYLIPFLVGACALAIVGSLIAYPVMRWAITRYRGLKNS"
     gene            complement(9771..10817)
                     /gene="thiL"
                     /locus_tag="VLA60_09520"
     CDS             complement(9771..10817)
                     /gene="thiL"
                     /locus_tag="VLA60_09520"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HSF09644.1"
                     /translation="MPPSRRQALVSTIGEFQLIRALLTGGFSLPGPHTLIGMGDDAAV
                     LAHPSSAHLVISTDLLVEHIHFSKNTSSFFDLGYKAAVANLSDIAAMGAIPTYILVAV
                     ALPSHVRYGDWKDLYRGLSVPCKAHGVQLVGGDTSASRSSLFLAITILGQVKPGQALT
                     RGGAQEGDIIYVSGSLGNSAAGLDYLSHHPSRGKNSKLRQPVKFLVGRHLQPTPRIAL
                     GRLLSSRRLASAALDLSDGLSGDLEHLCQQSRVGALIQEASLPMSPHLIAYASKMRVD
                     PLSWALHGGEEYELLFTVSPKKQHRLELAIKQLGIPATAIGVITPRRSGVQIAHQDGR
                     TQKLESQSYVHFSE"
     gene            complement(10827..>12825)
                     /gene="lon"
                     /locus_tag="VLA60_09525"
     CDS             complement(10827..>12825)
                     /gene="lon"
                     /locus_tag="VLA60_09525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSF09645.1"
                     /translation="MPDVLTVIENLDDPGRLADIIASNLGLNIETTQGVLEIDDPLLR
                     LRRVNEILGNEQDVLSMQQKIQAEAKGEMDKTQREYFLREQLKAIQKELGELDDRSEE
                     VAEFRRKIQEASMPDKVLKEAEKQLKRLEKTHQDTAEAGTIRTYLEWMVELPWSRHSQ
                     DNLDIPQANQVLQEDHYDLERVKERILEYLAVRKLKAKMKGPILCFVGPPGVGKTSLG
                     KSIARAMGREFVRMSLGGTRDEAEIRGHRRTYVGALPGRIVQGIKQAGTNNPVFMLDE
                     VDKIGTDFRGDPSAALLEVLDPEQNHAFVDHYLGVPVDLSEVMFITTANLTDPILSAL
                     RDRMEIIEIPGYSEEEKLGIAQRYLIPRQLEEHGITPEHLLLTDPCLARIISQYTKEA
                     GVRNLERELANVMRKVAKRIAEGKIKRYQVTPRNLHHYLGVPKYVPDQEQEKDEVGVS
                     TGLAWTETGGDVLYIEASAIKGKGLLTLTGHLGEVMKESAHAALTYIRSQAKSLGINP
                     EIFAKQDIHVHVPAGAIPKDGPSAGITMATALASCLSNQPVKRDLAMTGEVTLRGRIL
                     AIGGLKEKVLAAKRAHIKTIVIPKRNQKDLDDIPKHLLRGLEFIYVENMSEVLKVALH
                     TKHRHDMNSSMTHGHSSSGRRKKVAAPVTRRPLRQSITKTR"
BASE COUNT         3218 a         2904 c         3354 g         3349 t
ORIGIN      
        1 cgtcgtttgg ctccccagct cgatcagttg tgtattcaag ctgacaccgt agtggatatg
       61 tgtcgtcaaa ggcctatccc ggttaataga ttaaaggcgg gccagtacca attaaaagtt
      121 gacacactca cgatgcaaca gatcgccaga ccatcattgg cctttggccc tttggcgatg
      181 gccttttttc gagatacctt tagcttggag tccttgaccc ttaccggaat ggccgaggaa
      241 catgctcgag cgtatcatgt atttcgcact cgcggggaag aaaattatag gcggttggaa
      301 ggaatgtttt ctcatgatcc aaaggggggt ctgtatgcac gtacaattgg atgaagaaat
      361 ctgggaagtg ggtgatcgtg tccaactagg ggaggttctg gcaaatttga gcgaccgcgc
      421 tcaagccaaa ggatgtttgg tgacggaatt aatagtcggt aatcggaaga tgacggatcg
      481 ggagttagtg ccccccaccc tggctaaagt ggcaagttcg tttggatcga tagcagccgt
      541 gagcaaacat gtggaaacca tcgtacaata ttctgaaaaa acgggccaaa aattcggagc
      601 acagctacgc gaacaaggac aaaagtttgt tgatgctttc cgacaagggg atgggagata
      661 tcaccagtta gaccaatggt tcggccaaat agccgattac cttgaatggc tacaggttca
      721 tgaatctatg gggctaaaaa agccagaggt cacccatgac ctatcgttct ggatcaagga
      781 attactgaat gctcgtgaaa acgaagatgg ggttcgaatc gcagacatgt tggagtatga
      841 agtgattcct cgtctttccc gaaatattac ccttctcctg taacgcaagc tttctcttcc
      901 ttccatttcc tatagcttcc tacggagtta ccactggagg atgtggaatg aagtaatttc
      961 tgtttactcg atcaggtcac tgcgtggatt cccaatgggg ttctggtaga agacaagtga
     1021 ttggctagca tcccctcaag ttttcctctt gatggtcgat acataccggt ggaagtggaa
     1081 attggttcat ttcacacatt tcgaggaggc caatcggctc aactcattta ttgacttcct
     1141 caagggacgg atcctttgag gtgcagccat caaggaggaa agtatggcac ttgtcgttaa
     1201 caacaacatc gcatcactca acgctcaacg gaatttgggc gtgtccagtt ctcaattgga
     1261 agggtccgtc gcccgtcttt cttcgggttt acgaattacc cgagctgcag atgatgccgc
     1321 aggtttagga atttccgaaa ctttgcgtgc tcagattcgt agtattaatc aagcggtcag
     1381 aaattccaat gacggtataa gtttactcca gattgcagac ggtggtgcgg aaggtatcgg
     1441 taatttgttg ggtcggcttc gggaactcgc cgagcagtcg gccagtggaa ttcttggctc
     1501 gaatgaacgg tctttcctcg accaggaatt tgtggcccta cgttcggaaa tcgatcgaat
     1561 ttcttctgtg actgagttca atggggtaaa actgttaagt ggaacaggaa atgattcgtt
     1621 gagcatccaa atcggtttcc ggagttcagc gaatgataca ctttctctgt cactcaatga
     1681 tttgaacacg aataccttaa atctcgcctc gaccaatgtg tcgacttccg gcaatgcctt
     1741 agacgcactg tccaatatcg atagtgccat tagtgctgtg gcgtctgctc gagcaaatat
     1801 cgggtccttg caaaacagaa tcgatgctgc tgttgggaat ctgcaagtgg cgaatgaaaa
     1861 tatcactgct gctgaatcac ggattcgtga tgctgatatt gcctttgagt cggccaagtt
     1921 cgtcaggaat caaatattgg ttcaggcagg aacctcaata ttggctcagg ccaatacctt
     1981 gcctcagcag gccctcgcct tgctgcagta attcgtcata aggagaaacc ggggtttagc
     2041 cccggtttct cctttaagaa agttggggtt aacccattat gatttcaaca ctttctgaca
     2101 gcaccttttt cataaatatc tcaattggaa atgagattcg cctacgggat ggacagaagg
     2161 ccatcacggg ggatactcat cgcaaatcag agacaaaaca gccagtttat gcctcagact
     2221 ttactaagga ttcaacggcc aatgaaacat tgacggcgga gcaagtccaa gcaacggtgg
     2281 ctcgccttca agaaagggtg aggcagattg atccccggat tgagatctct ttagaaaagg
     2341 atattaatca ggtgattttt cgagtcgtgg ataaagaatc aggtgacctt atccgacaaa
     2401 taccttcaga acagatggta gatcttgcgc gattattttc cggacagtca ggtctgtttg
     2461 tagaagagaa catttagtct acgtttaatt cacttgcagt tcgtgagccc agagtccctg
     2521 cccctgggag aacgagttct cgtgaaacaa tggaagaccg ttaagcgttt cgtcatgttt
     2581 tagagaatga cgaaggaata tcggatggct attagttttg gtgggcttgg caatggcgtt
     2641 gattttggac cggttgtcga tgcgctagtt caagcagaac gccttcccat tgatgccctg
     2701 acccagaaaa agcttcaggc ccaaaaaaaa caaaccgaat tgggtttgtt aggggctaaa
     2761 ttactcagct ttcaaagttt ggcgagcagt ctgcgcaata gagtcagttt taataaaaac
     2821 caagttaatg tggcctcacc tagtgcccaa acccctttat cggcaactgt ttcttcatct
     2881 gcggccccag gcacctatca ggtgactgtg aatcagttgg cctccgcaca tcaaataatt
     2941 tcgaaggctt ccacggcagt ttcccaaact gatgcggata ttgtcggtgg cgaatccggg
     3001 acatttcaat ttaaagtggg tactggctcg attcaaacgg tgaatttaga tgccacgggg
     3061 acactggatg atttacgagt cgcgatcaat gatctgggag ccggagtcag tgcttcaatt
     3121 ttgaataccg ggagtgaagc ttcgccccaa tttcgtcttg tgttgtcagc gaatgagact
     3181 gggcttgacc aggccatcac aattgtggcc gacgatacaa cattggatat ggcgacgacg
     3241 ggagtggata catttcaagc cgctcaggat tccgaagttg tcttgggggt aacggatgcc
     3301 ggagccggga ccacggccat tacgatcaac cgttcgacca atacacttac cgatgtcgtc
     3361 gcaggcctca ccatcaatct tcaagcggtg gatgccacga atcctgtcac gatttcggtg
     3421 agtcaggata atggagctgt caaagaagcg atctcagatt tcgtgagtgg gtacaatgag
     3481 atcgtagcat ttgtcaatga gcgaaccttt tataataccg aaaccaaaga gcgagggatc
     3541 tttgttggag aaagtttagc tcgaaccgtt cttgatcggg tcagggaatc cgttttttcc
     3601 caaatcagcg ggttgacgac atattcaggc gcttcccaaa ttgggtttga gacccaaact
     3661 gccgatggga cgattaagct gaacgaaaca acattagatt cggccttgaa tacaaacttt
     3721 tccgccgtgc gtgatctgtt tgtcaagaat accaccaccg gtaccagggg aattgcggat
     3781 ttggccgtga atgccattga tggattagat gatatcgaca cgggatctct cacacgccgc
     3841 caaaatgcct taacgaaaca agtcaaagat ttcaccaatc aaattacatt aaaggaagaa
     3901 gccttggccc gttttgagca acaacaacgt ttaaagtttg ccaatttaga tggattgttg
     3961 gcctcgttgc aaagtcaatt gaaacagctg cagaacgctt tcccgtccac taatggctaa
     4021 agagaggatg atcatatgat gcttggtgct caggcttatg caaacacgca aatccaaact
     4081 gcgtctaaca tacaagtgat tgtattgctt tatgatgggg ccatttcttc tatgaagctc
     4141 gcacaagagg ggatggccac cctgaattac caggataagg cccgatttct cgatcgtgcc
     4201 cttcgagtga tcggggaatt gtcggcctcc ctcaatatgg aagatggcgg ggggatcgca
     4261 aaggatttac gtcgtctgta tgaatacatt caatttgaat tgactcaagc caacttaaaa
     4321 aatgagcccc gtcgcttaca aggacctatc cggtgccttt cgcttattcg ggaagcctgg
     4381 gtggatttgg ctgttcaagg aaccaagccg caggcggtcg ggatctaggc ctccgtgtcc
     4441 tatgggtaaa gttaactgtc ttgtcgaaca gctcacccaa gatgccattg aggccgcccg
     4501 acatggtcaa tgggaacagg tcattgttct ctacgatcag cgcctatccc aaggaccaca
     4561 acaaaccctt tctttaaata ccatccaagt cctcatggaa tctgaccaat ggctcattgc
     4621 cagggttaaa gaagtacaag cggcaatcaa ccaacatctg aatgctattc aggatcaacg
     4681 cagaaagtta ggtgttctta agcgacagtg gggagaaaac ccgacaaccc cggttcgaca
     4741 tcttctgacc atttgactgt ggaggggaat gttgaatggt ttttcatgcg ctgcaccctt
     4801 gggtttccgc caaagatcac cctccaatat ttgtgcataa catgacggaa tttccccgtc
     4861 acaattttga cgaaaagagt caaggtactt ctaatcggta aagaaaatgg cttctatgtt
     4921 ttcttccgct ctacatttct ctggaaaaac agccgaaagg aaagataggc tgtccaggta
     4981 tgactattgc gagattgcat ctttcaagga tgagatctcc atttttagag ggtgccacgg
     5041 ctggttgttc aggcctctat ttaaaggaac gacaggatga ctcaacaatc ggcatcatct
     5101 gcaaaatctt tgtcagtatt gaccgaaaat tctgtacaaa acgggaaatt attacattcg
     5161 ctctccaata aattgctgcc tattgtggtg ttttcccagt tagcgctacg tcggtgcgaa
     5221 gatgaacagc tgaagcgaca attggagaaa attcatgggg ctgccgaaca agccagggat
     5281 attcttgtgg aaattcgcag ccttcacgca gcgaggaatc cctagtatga aagcagtgtt
     5341 ttcggactcc atagttttgc tattccctgg attatgtgtg atgacaggac gattcttata
     5401 aaggtggctt atggatatcg tggcttctga ggatcgacgt caagattgcc gtgtagatgt
     5461 gcctgtatgt ctgtgggttg agattcctct tgccttctcc tatgaaatga ttgatcttga
     5521 tggagcagaa ttcagccagt ggcaatggga tgagctggcg gtctccccgg atttagtcaa
     5581 ggccatggtg gatgaaaagg atcttactat ccgtgatcct ttgctcttac agatggtcac
     5641 gcgtattgac tggatgttga catccattct ccggacatta ggaaaagata aaaatctcaa
     5701 gggggccatt ccagaattaa cgacagtgag cctatgcggt tccggaatga agttttattc
     5761 tgaagactca tatccccttg attcttttct cgttctacgc ctcattttgc ggccttttat
     5821 accgattcag gccgttggga aaattgtcag ggtaaatgct aaatcaaaag gcaaggaaca
     5881 aggatttgag atcgcggtgg aattcaccaa aatttcttct gatgatcgcg aagcgattat
     5941 tcgacacaca cttcggtctc aagccaccat gcaacggttt cgcctcaagc aacctgtcga
     6001 ttccgtcctt gactagggct ccggtaattt tctggtgtct ggtgtcctgt ctcgtgctaa
     6061 tggcaggatg tcagagacca ccggagattc ccgtttctcc ggcttcatca acttcatccc
     6121 ccattccctc tctacacccg cgtgttctca ggcgaccgca tagcgttgct ttccttcctt
     6181 tggaaaatat gagccgttat tcccatctaa attggctggg tcgcggactt caggaaatgc
     6241 tggtcagcga tttggcgcaa tggccacaac ttgaggtggt atcgcgagat gcattgggtt
     6301 cggtcttacg ggagcaatgg cttcaacaaa aaggattttc atcagttgag aatacagtcg
     6361 gtcttggaca tttgaaagga gtacggtatc tcattcaagg aagaattttc ttgcagcagg
     6421 acactctttc cgtggatctg cagattgtcg atgtagaaac tggggtggtc gtggcctcag
     6481 tcaacgctca gggggtagag tcggatattc ccggtttgga gcaagacctt gtgactcacc
     6541 tcattggtgt ctttgaccct tctctcgatg gagttagccg gtcaatggtc ggtcaaagtt
     6601 cggaggaccg gcagccgcac tttagggggg atcctggagt tgacaagaaa ggacgaccgg
     6661 tgatttcctt cgaaccaaat ccttcatcta tggtgtctca tgttgatgca tttctatcat
     6721 tggaaaaact cacgtatcag cgaagggagg cgtatcggtt agcagaaacg atttgggatg
     6781 agggatgtgt tgttgaaatg gggcagcccc tgtatcaggt ttggcacttt tcgattgatc
     6841 ccacgaggtc tgtgccggtc atggctattc ctctttcatt gtttttctct tcacaccgga
     6901 tttccaatat tttcgagcaa gtgcagaagt caggaaccga ttcggggggg aatgtagatt
     6961 ctgatggatt tagaacgacc cttgatgagg agtcaggagc ccagtcatta tttattgaac
     7021 atttccaaaa gccccggcgc gtatttatcc gtgctcttaa tgaaaagcag gaagtgctgg
     7081 cgattttttc cgattggggg tggcggaccg aacgtaaatt ttcaatgcga gggactcaag
     7141 gggtatccct gcctatgtgg ccgaatccgt ttgttaccgg gctcgtcaaa ttcccggttg
     7201 attgggtcga gcgggaaggg caacatgtga catttgatag cgtggcggtt cctgtgccgg
     7261 acgagcacgc tcttgttgtg ctggagccca tcggagagtt caaaggagag aaaagccaga
     7321 tttcgcgcct ggttgattcg gaagacattc ttcttcatgg gttagaaact ctgatcaaat
     7381 ccaattgggc tcctgcgata acggaagggc tgccaatgag ggggtatctc ccagggaata
     7441 aacggacggc ggtgggtgtc gtccatgtgc aaaaagggaa aattcgtgac atccaatttc
     7501 aaagctggcc tgaggatccc tttttcctgg aaagcttaca agatcttcaa ttaaagttat
     7561 tgggggcctg cccggagtgt caggattccg ctcaggctgt cccccatctc tccgatccgg
     7621 ttaaaacatt tcgcttacaa ctcaccctgg tgaaagacat ttacgccctc catcttggca
     7681 gtcgctctca ttaacctggc acgcgatttt ctcattccaa tggatctata tgtggggcaa
     7741 ctgtcccggt tataggcatt taaaccggtt gaattaaagt tggccagtgt cgacgcaggg
     7801 tttcctcggc agctttggca aggatttcat cgatggaatg atcttggctg aatactcgca
     7861 gggtgatcat ccgggctgga agatactgga gatatttctg aaggctgtct ttttcgaccg
     7921 tgcctttggc ggggttccag acaaatattt gaaagttccc catattgagc aaattaatgg
     7981 ggcgagggtc cttatgagca aagtcaactc gaatatcctg gtgacgatat ttagtaggta
     8041 acagtcgacg catccggttc agaaagacct gaggttgaaa ggatgatgat gccggctgaa
     8101 tgatggggag cagcgtgctg aatgcggttt tccacttcac gtgtcttccg ataatttgac
     8161 tccactccat tcccagttgt cgtttatgca tggaacgagc gtgcttccat gaacgcaccg
     8221 tttcgatgac gtgccaatcg gttaactgga gataatgagg taaggattgg agaggatttt
     8281 ttttgcacag gatctccatg gtgggttgaa aaatttcctg gagatgaatg tcgatcgccc
     8341 gtgtggtgcg atggtagtag acattggaat agaggtaaag cctggcgtgt aaaaacattt
     8401 gtagcgcagg tagtcctgat tggtggattg tgagtccctg tgaggaaaag aaggtgtaat
     8461 gaaggagtcg ttcaatatct acagggccaa tggctactcc gcacatgtag gaatccctga
     8521 ggacgtagtc gcaattgtcc ggggtataaa ttccgccgag aaggggctgt aaggcgatca
     8581 cccacggcgg cagacgcttt gtcgatttgt gaggatcctt gagaatcagg aaggcgatat
     8641 gttccggatc caattgctct ctcggaccaa atggcccgga cggactccga cagatctttt
     8701 tgattattgg ccccaaatgg tcacgaataa tgtgttggcc gaccacttca tgcgtcaggt
     8761 taaattgatg caggaaatga tggtcgaaaa aatggcagaa cgggccatgt ccgatatcat
     8821 ggagcagggc cgttatacga ataaattctt ccagaaaatg gcacgatggt acctcggaga
     8881 ccgtttggca aagcgacgga tagaggcgag ccataaatcg acctgccagg tgcatggtcc
     8941 cgagggagtg ctggaagcgg gtatgctcgg cgcccggata aacccaatgc gcactttgaa
     9001 gttggagaat ataccgaaga cgttgaaccc agggagaatc aatcaaatcc ttttcggtct
     9061 gttccgtaga atgtggttct tgatagggaa cggtgatgga aatatagccg tgaatagggt
     9121 cggcaagaag cgagacgcca tcaaacgggg atgagagtgg ttctgcctcg gacataggct
     9181 aagcgtcttt ccctgacctc aaacttttgc ctgttggagt gtgccgaatt gtcagaaaat
     9241 gcatagcata aaaagtattc acggtgagga tttgctggtg cgccatgttt attattttgc
     9301 taggaattct tgagccctcg atatcgggtg atggcccatc gcatgacggg ataggcgatc
     9361 aacgatccga caatcgccag ggcacaggcc ccaacgagaa aaggaatgag gtagggggaa
     9421 agcatgtcaa atatattgtc caccctcatc tgaccccact caatcgtgac tggtgaggat
     9481 acgcccatca acagcattcc ggtgtagaga ctggcggcta agattgggat aagtgtccag
     9541 ggattgttga tcaaggaccc cagaaagagc gctaaaaaat tcagccggaa ggcccaggcg
     9601 cagaatacca cgctggcggt gtggaacaaa taatgtggtg cgaaggcgat aaacacacct
     9661 agagcaaatg ccaaggctgt gcggtgagga gtttcccgaa gatgtaagac ctgagtcaat
     9721 tgttggcgga cagcttcaag cgttaccatg gacagcttcc tggcattctc ttactccgaa
     9781 aaatgcacat aactttgaga ttccaatttt tgagttctgc cgtcttgatg cgctatttga
     9841 actcctgaac gacgaggagt aatgacacca atggcagtgg caggaatccc tagttgtttg
     9901 attgccaatt ccaggcggtg ttgttttttg ggagacacgg taaacagcaa ttcatattct
     9961 tctcctccat ggagagccca ggatagagga tctaccctca tcttggaagc atatgcgatg
    10021 agatgaggtg acatgggaag gctggcctcc tgaatgaggg cacccactcg gctttgttgg
    10081 cacaaatgtt cgagatctcc tgagaggcca tcagataggt ccaaggccgc cgaagccaaa
    10141 cggcgggaag acagcaaccg tcctaatgca atacgagggg tgggctggag atggcgccca
    10201 actaaaaact tcacgggttg ccgcaatttt gaattctttc cgcgtgaggg gtgatgggac
    10261 agatagtcca atcctgcagc tgagttacct aacgacccac tgacatagat gatatctcct
    10321 tcctgagccc caccccgtgt taacgcctgg ccgggtttaa cctgtccgag tatggtgatt
    10381 gccagaaaca aagaggaacg tgatgctgaa gtatccccac ccacaagttg aaccccatgg
    10441 gctttgcagg gaacggaaag cccgcgatac aagtccttcc agtctccata tctcacatga
    10501 gagggaaggg ctacggccac caggatatat gtgggaatgg ctcccattgc ggcgatgtcg
    10561 ctgagattgg ctacggctgc cttgtagcca aggtcaaaaa atgaagaggt atttttggag
    10621 aaatgaatgt gttcaacgag gaggtcggtg gatatgacga gatgtgccga tgaggggtgg
    10681 gccagaaccg ccgcatcgtc tcccatgcca atgagggtat gtggaccggg tagggaaaac
    10741 ccaccagtca gcagagcgcg gataagttgg aattccccta ttgttgacac gagcgcctgg
    10801 cggcgggaag ggggcatgat aaaaagtcag cgtgtcttgg taatggattg tcgtaacggg
    10861 cgacgggtaa ccggggccgc aacttttttt cgacggccgg aagaactgtg tccatgagtc
    10921 attgacgagt tcatgtcgtg ccgatgtttg gtgtgaaggg cgactttgag aacttccgac
    10981 atattctcca cataaataaa ctcaagaccc cgtagtagat gtttgggaat atcatcgagg
    11041 tctttctgat ttcttttagg gataacaatg gttttgatat gagcccgttt agcggctaga
    11101 actttttctt ttaacccgcc aatggccagt attctgccgc gaagtgtgac ttcccctgtc
    11161 atcgccaagt ctcgtttgac aggttgattg ctgagacagg atgcaagggc cgtcgccatc
    11221 gtaatgcctg cggagggtcc gtctttgggg atggccccgg ccggaacgtg cacgtgaatg
    11281 tcctgtttag cgaaaatctc aggattgatt cccaatgact tggcctggga gcgaatatag
    11341 gtaagtgcgg catgagcaga ttccttcatg acctcgccaa gatgacctgt taaggtcaag
    11401 agcccttttc ccttgatggc cgaagcttcg atgtacaaga catccccacc ggtttctgtc
    11461 catgcgagtc ctgttgagac gccgacttca tctttttcct gttcctgatc cggcacatat
    11521 ttaggaacac cgaggtagtg atgaaggttt cggggagtga cctgatagcg tttgatcttt
    11581 ccttcggcaa tgcgtttcgc cacttttcgc atgacattgg cgagttcacg ctccagatta
    11641 cgaactccgg cctccttggt atattgagaa ataatgcgcg ccagacacgg atcggtgagg
    11701 agtaagtgtt ctggcgtgat tccgtgttct tctaactgac gcgggatcag gtagcgttga
    11761 gcaatgccaa gcttttcttc ctccgaatac cctgggattt caatgatctc cattcggtcc
    11821 cgtagtgcgg aaagaatggg atccgtcaaa tttgcggtcg taatgaacat gacttcactc
    11881 aggtcaacgg ggactccgag atagtgatcg acaaacgcat gattttgttc cggatcaagc
    11941 acctccagca aggcagcgga gggatccccg cggaaatcgg tcccgatctt atcgacctca
    12001 tctaacatga acacggggtt attggtccct gcctgtttga tcccctggac aatccgtccc
    12061 ggcaatgctc cgacataggt tcggcgatgc cctcgaattt cggcttcgtc tcgtgttcct
    12121 cctaaactca tgcgaacgaa ttcccgtccc atggcacgag caatcgattt ccctaaagaa
    12181 gttttgccga ctccgggagg gccaacaaaa cacaagatgg gtcctttcat tttggctttg
    12241 agtttgcgca cggcgagata ttccagaatt cgttccttga cacgttccag gtcatagtga
    12301 tcctcctgca gtacctggtt tgcctggggg atgtcgaggt tatcctgaga atgacggctc
    12361 caaggtaatt ccaccatcca ttccaagtac gttcggatcg tcccggcctc ggccgtatcc
    12421 tggtgcgttt tttccagacg ttttagttgt ttctccgctt ctttcaggac cttgtccggc
    12481 atgctggctt cttgaatttt tcgacgaaat tcggccactt cttcagatcg gtcatcgagt
    12541 tctcccaact ctttctggat cgctttgagt tgttcgcgaa ggaagtattc tcgttgcgtt
    12601 ttatccattt cccccttggc ttcagcttgg attttctgtt gcatggacaa cacgtcctgc
    12661 tcattaccca ggatttcatt cactcgcctg agacggagaa gaggatcatc aatttcaagg
    12721 actccctgag tggtctcaat gttgagccca agattggaag cgataatgtc tgccagacgt
    12781 ccaggatcgt caagattttc aatgacggtc agcacatccg gcatc
//
LOCUS       DATIAL010000301         4051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_916288, whole genome shotgun sequence.
ACCESSION   DATIAL010000301 DATIAL010000000
VERSION     DATIAL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916288"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..669
                     /locus_tag="VLA60_09530"
     CDS             133..669
                     /locus_tag="VLA60_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSF09646.1"
                     /translation="MELKNLLTPWNWFKKEEEQSQSARSQNVSRSPDHPLARFHRDLD
                     QLFDNVFQGFPLSPGGKENGSSWGGFILPHVDISEDKKQYTITVEVPGVAEKDIQMTL
                     ADGTLMIQGEKRSEQEDKNKHYHRVERSYGSFQRMLSLPTDADENNVEAKFKNGVLTI
                     TIAKDPGAKPAVRKIAIT"
     gene            1117..1575
                     /locus_tag="VLA60_09535"
     CDS             1117..1575
                     /locus_tag="VLA60_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09647.1"
                     /translation="MKNIMMLFTVLVFTVGAVDYASGDTTKPSFQVDPIEWSINTAEF
                     HLTQAQKLEQEATDLAARVRELNQKVAKYEKKPYLDTKGFHRDGLKRIIGTTLQKVSD
                     LRDQVAWHREEASRLADLDQVPQDSASDPEDTPPTLRDASRSHSHSEIPS"
     gene            1724..1855
                     /locus_tag="VLA60_09540"
     CDS             1724..1855
                     /locus_tag="VLA60_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09648.1"
                     /translation="MLGEAIIAMLTLMFLMWSAAIWASFKEDADQKPGSLHTRKKVA"
     gene            complement(1982..2893)
                     /gene="nhaR"
                     /locus_tag="VLA60_09545"
     CDS             complement(1982..2893)
                     /gene="nhaR"
                     /locus_tag="VLA60_09545"
                     /inference="COORDINATES: protein motif:HMM:NF008284.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HSF09649.1"
                     /translation="MDWLNYHHLLYFWEVARKGSIKEACEELHVTQPTISSQLQALEE
                     SLGQQLFIRRPRRLLLTEAGRLVFRYADEIFSLGREMTQALSGQPPDRPIRLMVGVID
                     SLPKMVVYELLAAALKSESPTQIVCEEGKLERLLADLVIHELDVVLADTPIPPILRVQ
                     AYNHLLGESGVCFFATSDLAKVYRKGYPKSLNSAPFLLPKANTVLRQDLESWFALHDI
                     HPHLVGEFEDSAMQKAFGQAGKGVFPGSSIMKAEICRQYQVEVVGDAGHVKQRFYAHT
                     VDRRIKHPSVLAISEFAKQRTFGLARK"
     gene            complement(3177..3623)
                     /locus_tag="VLA60_09550"
     CDS             complement(3177..3623)
                     /locus_tag="VLA60_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09650.1"
                     /translation="MNKVVIQLPCPRKRKDWEWPNSLPHATILLATPLIYAMIIPLLV
                     FDFCVELYQRVVFPLLGLPLLSRREYIRLDRHRLPYLNPFQKAGCLYCGYANGLLHYA
                     SRIAAETEKVFCPIQHRTGGKFHPPAHHIDFAPYGDAKEFQRKMDI"
     gene            complement(3782..>4051)
                     /locus_tag="VLA60_09555"
     CDS             complement(3782..>4051)
                     /locus_tag="VLA60_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09651.1"
                     /translation="QRLAAEKAAELAKGVKGIVSLVRINPSRPDREIEKEVALYLLWS
                     PIVNIDGVDFSVNDRVVKLEGAGEHSAHILTLEQDIGNIMGGCES"
BASE COUNT         1116 a          974 c          954 g         1007 t
ORIGIN      
        1 tcctccgcgg aaattggtgc tacggaggaa ataggttatg gcgggttatt tagcacaagg
       61 ttgatacctc cggtgtgcca ggcataaaaa cccttcttat tcaaattatt cgacgaaagg
      121 aggacatcca tcatggagct caaaaatctt cttaccccct ggaattggtt caaaaaagag
      181 gaagaacaat cccaatcagc acgaagtcag aacgtatcca ggtcgcccga ccatcctctg
      241 gctcggttcc atcgtgacct cgaccaactg tttgacaacg tcttccaagg attcccccta
      301 tcgccgggag gaaaggaaaa cggaagttcg tggggaggct tcattctccc ccacgttgat
      361 atcagtgaag acaagaagca gtacaccatt accgtggagg tcccgggcgt tgctgagaaa
      421 gatattcaaa tgaccctcgc ggacggcacc ttaatgattc agggcgaaaa gcgttccgag
      481 caagaagaca agaataaaca ctatcatcga gtcgaacgtt cgtacgggtc gttccaacgt
      541 atgctgtctc ttccaacgga tgcggatgaa aataacgtcg aagcaaaatt taagaatggc
      601 gtcttgacca tcacgatcgc gaaagatcca ggcgcaaaac cggctgtgag aaaaattgcc
      661 ataacgtaag gctcttctgc cttcgttcac gttgtgaacc ggcggttggc agaacaacgc
      721 caactgccgg aaatcgcaac acgctttctc ttgggcaatc ctcttccgaa tggagtggca
      781 gttttgactg atcctctcgt caaatgtaag ccccactcga tattcttcgg tctcacctga
      841 tatttattct cacctttttc ccgtaatttc aaaaagggtt tatccttata gtgcccttgg
      901 acgtaaacgg ccatgatggg gaaacgtgga ttgaattttt cgattctgtt tgtaagtttt
      961 ttcaatttgc ctctttgctt ttaaggactt atcttttttt atagaagaaa ggataaaagc
     1021 ccagtcgaac cgatgatgaa aggcaacatg gaacacggta taggggaaat gaaattgagc
     1081 attcagattc tcaactcaaa ggaaggaggt catacaatga agaacatcat gatgttattc
     1141 accgtattag tcttcacggt tggggcggtg gactatgcgt ctggtgacac gacgaagcct
     1201 tcgtttcagg ttgaccccat agaatggtca atcaatacgg ctgagtttca cctgacccag
     1261 gcccaaaaac tcgagcaaga ggccacagac ttggcggcca gggtgaggga gttgaatcaa
     1321 aaagttgcca agtacgagaa gaagccctac ctggatacga agggatttca ccgggatggt
     1381 ctaaaaagga ttattgggac aactttacaa aaggtaagtg accttcggga tcaagtggcc
     1441 tggcatcgtg aggaagcctc acggttggcg gatcttgatc aagtgcctca agacagtgca
     1501 tctgacccag aagatacgcc ccctactttg cgggatgctt ctcgctcgca ctcgcacagt
     1561 gagattccat catgaatcaa ccgtgacgaa gtggattatt tgaagggttt gtgactaaaa
     1621 aaactttctt catactgaac cctgaaactt gattgttagg actgggaacc tattccgaaa
     1681 tgggagcagt tgaaatcatc acggaatcga aaggaggatg agcatgttag gagaagcaat
     1741 catcgccatg ctgacgctca tgtttctgat gtggagtgcc gcaatttggg cttcctttaa
     1801 ggaggatgcc gaccaaaaac ccgggtccct ccatacaagg aaaaaagtgg cctagtcgga
     1861 tgagggaggg agccgggcct catgttgtaa acggagcatt tctcttggga caaggaggat
     1921 aacgccatct ccataaaagt aaattatcac atgaccggtc cggctctttc ccatgatttc
     1981 cttattttct agccaatcca aaggttcttt gtttggcgaa ttctgaaatc gctagtactg
     2041 acgggtgttt aatccggcga tcgacggtgt gggcataaaa acgctgcttg acgtgacccg
     2101 catctcccac cacctcaacc tggtactggc gacaaatctc agccttcatg attgaagaac
     2161 cggggaaaac cccctttcct gcttgtccaa aggccttttg catcgcacta tcttcaaact
     2221 ctcccaccag gtgtggatga atatcatgaa gagcaaacca actttccaga tcttggcgaa
     2281 gaactgtatt ggctttgggt aataagaaag gcgctgaatt taaggacttc ggatacccct
     2341 ttcgataaac cttggccaga tctgaagtcg caaaaaaaca tacgccggat tcgccaagaa
     2401 gatggttata ggcttgaact cgaaggatcg gaggaatcgg ggtatcggcc agaaccacat
     2461 caagttcgtg gatgactaaa tccgccagga ggcgttcaag ttttccttct tcgcaaacaa
     2521 tctgagtagg actttctgac ttgagggccg ccgccaggag ttcatagacg accattttgg
     2581 gcagggagtc tatcactccc accatgagcc ggataggacg atcaggcggt tgtccgctca
     2641 gcgcctgggt catctcgcga cccagcgaga aaatttcatc ggcataccga aaaaccagac
     2701 gaccggcttc cgtaagaaga aggcgacgcg gccgtcgaat aaagagttgt tggccgaggg
     2761 actcttccaa tgcttggagt tggctactga tcgtgggttg cgtgacgtgc agttcttcgc
     2821 aggcttcttt aatactcccc tttcgagcca cctcccaaaa ataaagaagg tggtgataat
     2881 taagccaatc catcggttag cgatcactca aaaattgttc aggcaagttc acgcccctta
     2941 tccaaagtta atccttatgc tccttcgtag actcgtctga ctggtggtta gcggcataga
     3001 tcccccgaat ttccgaaacc aattcgcgct ggttctaata tttcctcaaa attctcacaa
     3061 gagatcatgg aaagtacaaa gaagccggag gaaaagcaaa cggtaatttg acgaaggtgt
     3121 tccccgaccg gactcagtca atcacatcga attggaattc aagtattgca tgtacgttag
     3181 atatccattt ttctttggaa ttccttggca tctccataag gagcaaaatc gatgtgatga
     3241 gccggtggat gaaatttccc gccggtccgg tgttgaatgg ggcagaacac tttttctgtt
     3301 tcggctgcaa ttcgagaggc atagtggagt aatccattgg catatccaca atacagacaa
     3361 ccggcttttt gaaacggatt cagataggga agacgatgcc ggtcaagccg gatatactct
     3421 cgacgcgata ggagcgggag tccgagtaaa gggaaaacca cccgctgata caattccaca
     3481 cagaaatcaa aaaccaatag agggatgatc atcgcataga tcaatggtgt ggccagaaga
     3541 attgtcgcat gaggtaggga atttggccat tcccaatctt tacgtttcct tgggcatggg
     3601 agctgtatca caaccttgtt cattttatga gacccccttg gcagcttctg ttggcctgac
     3661 cactgtctaa acgaacacat tcaaattcct acaacttgga cactccaccc cctggaccta
     3721 agttatccat catcctcaag cagcacgaga ttttctgctt ttcactgtca tttttgacac
     3781 atcaactttc acaacccccc attatatttc caatgtcctg ttccaatgtc aaaatatgcg
     3841 cgctatgctc gccagctcct tccaacttca caacacggtc atttacggaa aaatccacac
     3901 catcgatatt cacgataggc gaccacaata aatataaagc gacttctttt tcaatttccc
     3961 tatcgggacg tgaagggtta atcctcacaa ggctgactat gccctttacg cccttggcca
     4021 attcggctgc cttttccgcg gctaatcgct g
//
LOCUS       DATIAL010000302         4487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1023592, whole genome shotgun sequence.
ACCESSION   DATIAL010000302 DATIAL010000000
VERSION     DATIAL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023592"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1538)
                     /locus_tag="VLA60_09560"
     CDS             complement(144..1538)
                     /locus_tag="VLA60_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF09652.1"
                     /translation="MIIKQFHVESLGHASYLLGSEETGEEFVVDIQRDVEPYLKESRT
                     QGMTIAYALETHQHNDYVTGIRELAERIPLQCLAGRHAKLGYDARPMADGERLTIGEI
                     DLQFLHTPGHTPEHISVLVTDRSRGKEPVLLLSGGALLVGDVARPDLLGGTEEAAHHA
                     EVMCKMLQEKILPLPDYVEVYPTHVSGSLCGGNIGSRLSTTIGYERRMNPLLADLDNK
                     DHFVKHCLDLKHLPGVPPYWPRMRKMNTEGPPLLGTLREPPALTPDQFARQQEEGCIV
                     VDCRQAEGFTTHIPGALNVGLGSSFATWAGTVIPEDSRILLVLDHPADLWDVCWQLLR
                     IGYPTPAGWLSGGMFAWRTAAKPLHQLMQWSVWDLQAHLETDSDLEVLDVRQPGEWAG
                     GHIKNARFVTGSELPKRVQEIRTKFQVAVICGSGYRSSVAASFLQHHAYRNIVNVLGG
                     MSAWKEANLPIVKD"
     gene            complement(1580..2068)
                     /locus_tag="VLA60_09565"
     CDS             complement(1580..2068)
                     /locus_tag="VLA60_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09653.1"
                     /translation="MITSPTTTRVEEHTDADINEEIYNRTKNNVFLYASAGPSAIDRR
                     LRELEMEWDIERVLEANAASFCLMGLALGAFIDRRWFIFPGAVAGFLLQHAIQGWCPP
                     VPLFRRMGIRTAHEIQQERHALKALRGDYRDVAEIKAEVNGADALQVTRAFEAAGNDS
                     ST"
     gene            2705..>4487
                     /locus_tag="VLA60_09570"
     CDS             2705..>4487
                     /locus_tag="VLA60_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09654.1"
                     /translation="MNEWKLIFDNWDIKNQQAREALCTLGNGYWATRGACEEARQHDG
                     HYPGTYIAGAYDRCERIIAGETVSHETIVNWPNWLYLTYAMDNGQWFSMDQVQVNTYR
                     QELDLQRGMLSRYIQFTEPKGRETILQSIRLVHMEHPHLAALQWTIIPLNWAGRLQVR
                     SALDSMISNENVERYREMNAKHFRLLDSGFHKQGWLFAVTETLQSHIRLAQGLRTQVS
                     GGNLLLQRNLQEMDMTIQEFVLDIQEQTPLQVDKTVALYHSRDRGISEPLEAVHHSLA
                     SAGSFEQLLCSHQKAWERIWDMSDIRIQNRPEDQLMLRLHLFHLLQTASPHLTDLDAS
                     LPARGLTGEMYQGHIFWDDVFALPFLIVRVPRVAHAMLLYRSRRQQQAGAQAQQNGYQ
                     GMRFPWRSGSDGSEQTPRFQWNPYSQHWIEDHTHLQCHQNAAIVYQIWKYYQATADLE
                     FLQSHGGLLTLQIARFWSSLATLDDMSGRYHIRGVMGPDEFHTGYPDNPTPGLQDNAY
                     TNVMAVWTLQCARQVLQTLPDYSRQQLMEIVSIDHLDLEHWENITRHMFVPFHEDDLI
                     SQFAGYEKLHELDVNAYQARYGDIRRID"
BASE COUNT         1104 a         1241 c         1099 g         1043 t
ORIGIN      
        1 aattaaactc agcggaaggt attcgctccc tcaggaacgg tttttaacct ccatctgatt
       61 cttgttttcc tcactaccaa gtctttagaa aaaaaacagt catggggaat tgtgcgacgc
      121 cctctaccgg ccggaatggc ggatcaatcc ttgacaatcg gaagattagc ctctttccag
      181 gcgctcatcc ctcccaacac attcacgatg ttccgatagg catgatgttg aaggaagctt
      241 gcagcaaccg acgagcggta tccgcttcca cagatgaccg cgacctggaa cttcgttctg
      301 atttcctgaa cacgtttcgg gagttcgctg cccgtcacaa atcgcgcgtt tttaatgtgc
      361 ccgccggccc actcccccgg ctggcgaacg tccaatacct ccaggtcaga atctgtctct
      421 agatgcgctt gaagatccca cacggaccac tgcattaact gatgaagtgg tttggctgct
      481 gtacgccatg cgaacatccc gcctgacagc caacctgcgg gtgtaggata cccgattcgg
      541 agaagctgcc agcacacgtc ccaaagatct gccggatgat ccaaaacaag taaaatacgg
      601 gaatcctcag ggatgaccgt accggcccat gtcgcaaacg acgatcccag gccaacgttc
      661 agggcaccgg gtatgtgagt cgtaaaccct tccgcttggc ggcaatcaac gacaatgcac
      721 ccttcttcct gttgtctggc aaactgatcc ggtgtgagtg caggcggttc gcgcaaggtt
      781 ccaagaagag gggggccttc ggtattcatt ttccgcatcc tgggccaata gggcggcaca
      841 ccgggaagat gctttaaatc caggcagtgt ttgacgaaat ggtctttgtt gtccaggtcg
      901 gccaaaagag gattcatccg gcgttcgtac ccgatcgtcg tggacaacct gctcccgata
      961 ttccccccac acagagagcc actcacatgc gtgggataga cttccacata gtccggaagc
     1021 ggaagaattt tttcctggag cattttgcac atgacctctg cgtgatgagc cgcttcttcc
     1081 gttccaccca aaagatcggg tctggccaca tccccgacca acaaggctcc cccggaaagg
     1141 agcagaacgg gttctttacc ccgcgagcgg tctgtcacca gaacgctgat gtgttcgggg
     1201 gtatgtcccg gcgtatgcag aaattgaaga tctatttctc caattgttaa acgctctcca
     1261 tcggccatgg gacgcgcgtc atatccgagc ttggcatgtc gacccgctaa gcattggaga
     1321 ggaatccgtt cggctaattc acgaattccc gtcacatagt cgttgtgctg atgagtctcc
     1381 agggcatacg caatcgtcat cccctgtgtg cgggattctt tgagataggg ttccacatcc
     1441 cgctggatat ccaccacgaa ttcttctccg gtttcctccg aaccgagaag ataggaggcg
     1501 tgccccaggc tttccacatg aaattgttta atgatcatga gttgtggagg atgaatgtgc
     1561 cgaaactgct cattaacgtc taggtggagg agtcatttcc ggccgcctca aatgctcgag
     1621 tcacctgcag agcgtccgcc ccattgactt cggcctttat ttcggccacg tcccgataat
     1681 ctccccgcaa tgcctttaac gcatgacgct cctgttgaat ctcatgtgcg gtccgtattc
     1741 ccattcgccg gaacagggga accggcggac accaaccttg tatggcatgt tgcaggagaa
     1801 aaccggctac agcgccggga aatatgaacc accggcgatc aataaacgca cccaatgcca
     1861 ggcccatgag gcagaacgag gcagcattgg cttccagaac tctttcgata tcccattcca
     1921 tctcaagctc ccttaaccgt cgatcgatgg ccgaaggtcc ggccgaggca tacaaaaaaa
     1981 cgttattttt tgtgcggtta taaatttcct cattgatgtc agcgtcggta tgttcctcaa
     2041 ctcgtgtggt cgttggtgaa gttatcatac atggcctcca ttcttttctg tgggacgccg
     2101 cactcccatg ccgtgggcgg tcgttctgtg tacgtctgtg tagtccagaa gataaggaaa
     2161 gacaaaaggg ccaacaacta acaaaaaccc tggagtccgt tgaaacttca gggtttttgc
     2221 tagtagtcaa ttcaggccgc gtgggacaca cttggagtat atgggcagag gggggatgac
     2281 tcgggaacga aatcagccac acacttcaga ttctcatatt cgtgtgttat gaaaaaccac
     2341 ccccccatac cggaagaaga ggctgaaaag gacctggaca gcatttttca tgagattaaa
     2401 caggtctttc gtgtatctgg agtcaatctc aactttcgca catgggccac ctatccaacg
     2461 atatccacga ttcgagactg attcgcgtga aagatgcgag acacttcctc atgtgggatc
     2521 aaccggaaat cgtgatcgcg catctgctgg atttcttcga ccggaccgtg ccatcccggc
     2581 acctcatgat ctagctgctc atcatcatga ataccaccgc tgtgttacca atggatgtcc
     2641 cccatgccag ggcatagtga actgatatca agtcgaaatc gattcctcat cctgcaggcc
     2701 gtgtatgaat gagtggaaac tgatatttga caattgggat atcaaaaacc aacaagcccg
     2761 tgaagcactc tgtacactgg gtaacgggta ttgggccaca cgaggagcat gtgaagaagc
     2821 cagacaacac gacgggcatt atcctggcac ctatatagcc ggcgcctatg atcgatgcga
     2881 aagaatcatc gcgggagaaa cggtctctca tgaaacgatc gtgaactggc ccaattggct
     2941 gtacctgaca tacgccatgg ataatggcca atggttttcg atggaccagg ttcaggtgaa
     3001 cacctatcgt caggaattgg acctgcaacg gggcatgctc agccggtaca tacaatttac
     3061 cgaacctaaa ggacgagaaa ccattctcca atccatccgg ctggtgcata tggagcatcc
     3121 ccatcttgca gccctacaat ggaccattat tcctctgaac tgggctggtc gattgcaagt
     3181 gagatcggcc ttggattcca tgatcagcaa tgagaatgtt gaacggtatc gggagatgaa
     3241 cgccaaacat tttcgattgc tcgactcggg attccataag cagggatggc tctttgccgt
     3301 cacggaaacc ctccaatctc acattcggct ggcccaagga cttcgaacgc aagtctcagg
     3361 cggcaacctc ctccttcaac ggaatcttca agaaatggat atgaccatcc aagaatttgt
     3421 tcttgatatt caagagcaga ctcccctaca ggttgacaaa accgtcgcac tgtaccattc
     3481 acgcgaccgt ggcatttccg agccgcttga ggccgtgcac cacagcctgg cttcagccgg
     3541 atcgttcgaa cagttgctct gctcccatca aaaagcatgg gagcggatct gggatatgtc
     3601 cgacatccgg attcaaaacc gtccggaaga tcaattgatg ctccgtctcc acctctttca
     3661 tctcctgcaa acggcttctc cccatctgac agacctcgat gcgagccttc cggcaagagg
     3721 actaaccggc gaaatgtatc aaggccacat attctgggat gatgtatttg ccttaccgtt
     3781 tctgattgtg cgagtgcccc gggttgccca cgccatgctt ctgtatcggt cacgacgtca
     3841 acagcaagcc ggagctcagg cacagcagaa tggatatcag ggcatgcgat ttccctggag
     3901 aagtggcagt gacggctccg agcaaactcc ccgatttcaa tggaacccct attcccagca
     3961 ttggatcgag gaccataccc atctgcaatg ccatcagaat gccgccattg tctatcaaat
     4021 atggaaatac tatcaggcaa cagccgacct ggaatttctt cagtcccacg gcgggctgct
     4081 cacactccaa atcgcccgat tctggtccag tctggcaaca ctcgatgaca tgagcgggcg
     4141 atatcatatt cgcggggtca tgggacccga tgaatttcat accggatatc cggataaccc
     4201 cacgcccggc cttcaagaca atgcgtatac caatgtcatg gctgtttgga ccctgcaatg
     4261 cgcccgacag gttctccaga ctctccccga ctattcgcgc cagcaactta tggaaatcgt
     4321 ttcgattgat cacttggacc tggaacattg ggagaacatt acccgccata tgttcgtccc
     4381 ctttcatgag gacgatctca tcagccaatt cgcgggttat gaaaaacttc acgaattgga
     4441 tgtgaacgcg tatcaggccc ggtatggaga tatccggcga atcgatc
//
LOCUS       DATIAL010000303         2971 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_594274, whole genome shotgun sequence.
ACCESSION   DATIAL010000303 DATIAL010000000
VERSION     DATIAL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594274"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VLA60_09575"
     CDS             complement(<1..712)
                     /locus_tag="VLA60_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF09655.1"
                     /translation="MKKSINMVAGENIPYINEAFASLGNLTLLPGRSIKSSDLHNTNV
                     LLIRSITRVDHTLLEGTPVEFVGSASAGVDHIDEAYLKARNIGFTSAAGSNANSVAEY
                     VLASLLFLGKQQGFSLKGKTIGIIGVGNIGKLVKHKVEALGMLPVLHDPPLAESGQVE
                     HRSLAETLSCDVVTLHTPLTTDGPYPTYHMLNEQTLKWLNPSAIFINAARGEVVETHA
                     LLNAITENRIGPTIIDVWE"
     gene            complement(1063..2031)
                     /locus_tag="VLA60_09580"
     CDS             complement(1063..2031)
                     /locus_tag="VLA60_09580"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HSF09656.1"
                     /translation="MKFISYVLTFGLNLALITPAFSYEEITVSNGGTITGKVTLAGKE
                     PPALAYSLITNPDTDFCGRISTGTGWRLVDEFQVAPDGGLQNTVVFLEGISQGKPFPQ
                     TGAAKVTVEDCLFTPWVSVVQNEQPLHIVNMDPIIHDVQIYETAPFGSKVMLHRPLRL
                     NPFHPKNRIKDHQHNPGEAMLDTVEFSQGRRILYLECGFHTYMQSWRVAVDNPYYAIT
                     DAEGNFTLPDVPEGVYSLLAWHPGMGGFIKMDVVILANETLKTRMEFQNPIDRRMAHN
                     TMFSNYRFGTEVLEKEGAVYDVQATHETQDILSDQHGQTANTHSTH"
     gene            2030..2158
                     /locus_tag="VLA60_09585"
     CDS             2030..2158
                     /locus_tag="VLA60_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09657.1"
                     /translation="MEGSFGQLVHTDEWVKPIDTLILGNTLGTFPAPWKEEAGSLG"
     gene            2719..>2971
                     /locus_tag="VLA60_09590"
     CDS             2719..>2971
                     /locus_tag="VLA60_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF09658.1"
                     /translation="MAVQTLPSSVWSIVLAGGEGERIRPSIQQWLGYPVPKQYCTFVG
                     TRSMLQHTWDRADQIGLPRKKVTVVGRTHQQSLEHHCTRQ"
BASE COUNT          698 a          709 c          784 g          780 t
ORIGIN      
        1 cttcccacac gtcgatgatg gtcgggccga tacgattttc tgtgatggcg ttcagcagtg
       61 catgggtctc cacgacttcc ccacgcgcgg cattgatgaa gatcgctgat ggattcagcc
      121 atttgagtgt ctgttcattg agcatgtgat aggtcgggta ggggccgtcg gtggtcaagg
      181 gcgtatggag tgtcaccacg tcacaactga gcgtttcggc taacgaacgg tgctccactt
      241 ggcccgattc tgccagggga ggatcatgga ggaccgggag catccccagc gcctcgactt
      301 tatgcttgac cagtttgccg atatttccga cgccgataat gccaatggtt ttccctttga
      361 gtgaaaaccc ctgttgcttg cccaggaaca ataaggacgc caggacatat tcggccaccg
      421 agttggcatt cgatccggct gctgaggtaa agccgatatt tctggctttg aggtaggctt
      481 catcgatgtg gtccactcct gccgaggccg atccgacaaa ttcgacagga gtcccttcca
      541 ataaggtgtg atcaaccctc gtgatcgatc gaatgaggag aacattcgta ttgtgcaaat
      601 cgcttgattt gatggatcgc ccgggcagaa gagtcagatt tcccaagctg gcaaacgcct
      661 cgttgatata ggggatattt tctccggcta ccatgttgat cgattttttc atattttgtg
      721 cctttcagac gatgtttttc caaaactgca gcctatcgga actggccggt aggcgctagc
      781 cttgcttttg ttgttacatg gacccacccg atgcgagggc aggcaagaat cataagaaag
      841 aggggaaagg ggggcgggtc ttgtttttta aggaggcgag ttgaaaagaa tggggcctga
      901 cttattcagt aagacctatc cgccaggctg gttgatccaa gtggacatat taagagcacc
      961 ctacagagat aatagctcac tgatctgttt attccaatca agcacatgca ttaatagcct
     1021 ttctcaagaa ttagctagtc aggtccctag atgtaggcag tatcaatgcg ttgaatgcgt
     1081 attggccgtc tgcccatgtt ggtcgctgag gatgtcctgc gtctcatgag ttgcttgaac
     1141 atcataaacc gctccttcct tttccaggac ctcggtgcca aaccgataat tagagaacat
     1201 ggtattatgg gccatgcgcc ggtcgatggg attctggaat tccatcctgg tcttcaatgt
     1261 ttcgttggct aagatcacca catccatttt gataaatcct cccatgcccg gatgccaggc
     1321 caagagggag tagacgcctt cggggacatc agggagtgtg aaatttcctt ccgcatcggt
     1381 aatcgcgtaa tacgggttgt ctaccgcgac tctccagctc tgcatgtagg tgtgaaatcc
     1441 gcattccaag tagaggattc tgcggccctg ggagaattca accgtgtcga gcatcgcctc
     1501 tcccgggttg tgttgatgat ccttgattct gttcttggga tggaacggat tgaggcggag
     1561 tgggcgatga agcatgactt ttgagccgaa gggcgccgtt tcataaattt gcacatcatg
     1621 aataatggga tccatgttga cgatgtggag cggttgttcg ttttgtacaa ccgagaccca
     1681 gggcgtaaag aggcaatcct ccaccgtcac tttggctgca cctgtttggg ggaagggttt
     1741 gccttgagaa attccctcca aaaagacgac ggtattctgg agacccccat ccggcgccac
     1801 ttgaaattca tcgacgagac gccatcctgt tcctgtggaa atacgcccgc aaaagtccgt
     1861 atcgggattg gtaatcagac tgtaggccaa tgccgggggt tctttccccg ccaaggtcac
     1921 ctttccggta atggtgcctc cattagagac cgtgatttcc tcataactaa acgccggagt
     1981 aatcagggcg aggttgagac caaacgtgag aacatacgag atgaatttca tggaaggatc
     2041 ctttggtcaa ttagtccata cggatgaatg ggtcaaaccc atcgatacat tgatcttagg
     2101 aaatacctta ggaacatttc cagccccctg gaaagaagag gccgggtcat taggataatg
     2161 gatatgctgg tttcatgcca caggttgtgc atgcaggaac tccccttttc tgtccggttc
     2221 acaattatgc aaaagaaaag aagaaagaag ttcggtcagc ccccacctta agttgtgagg
     2281 gccgaatcca ccttgtttgg taaattgcgg ttagggcgga ctgagcgatg catccgggag
     2341 ggtcatttcc atcacacttt cgtaaagcat gggtttaaag ccatgtcatg tcctattcac
     2401 gacagagcat cagtcgcctg aaaattcttc ttgccatgcc atgaaggtcc aagccttcag
     2461 cctgtttggt tccgcacatt tctggctctg atggcttcgg cggaaagtta gaaaaatcaa
     2521 cgaattaagg gttcttctga ctctggcctt tcaattgctt acctttcaat tcattatccg
     2581 aagtgcaata ttaatcgctg gctcctcccc tgttatctga cataaactca gtttgtggtg
     2641 tctggaaaaa cgagtgtggg tgttgctcta tcgggcatgt tccattagct acgttttaca
     2701 atttccagga ggttcatcat ggctgttcag accctaccgt cctccgtgtg gtccatcgtg
     2761 ttggcaggtg gtgaagggga acgtatccga ccctcaatac agcagtggct cgggtatccg
     2821 gttccgaaac aatactgcac ctttgtcggt acccgttcta tgttgcaaca tacgtgggac
     2881 cgggccgacc aaatcgggct gccccggaag aaagtgacgg ttgttggtcg aacacatcag
     2941 cagagtttgg aacatcattg cacgaggcaa g
//
LOCUS       DATIAL010000304         2459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_701851, whole genome shotgun sequence.
ACCESSION   DATIAL010000304 DATIAL010000000
VERSION     DATIAL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701851"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1137
                     /locus_tag="VLA60_09595"
     CDS             220..1137
                     /locus_tag="VLA60_09595"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HSF09659.1"
                     /translation="MTFQDIILSLHQFWKSHHCILTQPYDTEKGAGTFNPATFLRAIG
                     PEPWRAAYIEPCRRPTDGRYGDNPNRLQHYFQYQVVLKPAPADSQDLYLQSLAHLGLD
                     PYQHDIQFLQDDWESPTLGAWGLGWEVRLDGMEITQFTYFQEIGGLELDPVTVELTYG
                     LERIAMYLQQVDNVYDLMWTEDVRYGELYHESEVQFSVYNFEAADVDLIQTSFDRYEK
                     ECQQLLEHPRGLVLPAYDCCMKCSHLFNLLDARGAISVAQRTGYIGRVRNLARKCAQA
                     YLGQREQMGFPLLRHAAASARPHRKQKVI"
     gene            1175..>2459
                     /gene="glyS"
                     /locus_tag="VLA60_09600"
     CDS             1175..>2459
                     /gene="glyS"
                     /locus_tag="VLA60_09600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HSF09660.1"
                     /translation="MATSRTTRKNVKAKSVSKSQPKTVPFLLEIGTEELPSAFFPKIL
                     DDLEFLAKEMFKSSRLMDDSLQMLNSSLQTFGTPRRLALVVEGVQLKQEPFLEEVIGP
                     PKAAAYDPAGNPTKAAEGFAKSQKVPIDWLKIKATPKGQYVYVDKTQPGQPAKRVLAD
                     AIPKLLAQLYFPKAMRWNASRVKFARPVRWIVAMLGKEVLSFEFAGVGSNRQTWGHRF
                     PQKKIRNISTGLKIPHASHYLETMKKAGLVVDPGERRKLIDAQVDKLAKSAKGEVDPE
                     TRHELTETAVYGVESPHAIIGAFNPDFLSVPKPVLISSMKEHQGFFSLMTKDGALLPK
                     FIAVTNMPWGNTPLMTKGNERVLAARLSDAQHFFKEDSKRPLHERVPALEGVLFHHKL
                     GTVGQKVDRVGHLIGWMAEQIGRKDLDEVCRRAALL"
BASE COUNT          621 a          557 c          650 g          631 t
ORIGIN      
        1 cttgagactc tagaaaaaaa caaggtgcgg acgtcaaaat cttctttcct gatctcttcc
       61 gcgtatgggt tgttttcaat ctgattatct tgactggtat agggtttatt gctacggtaa
      121 aatttttctc tctacgtgca gagaacgaac ggaattattc atggcatacc tcattgctcg
      181 gctgttccag ggtcaaccta ggggccaagg tcaagtgttg tgacctttca agatattatt
      241 ctctcccttc atcagttttg gaaatcccac cactgcattt tgactcagcc gtatgacact
      301 gaaaaagggg ctgggacgtt taatccggca acatttcttc gcgcgatcgg ccctgagccc
      361 tggcgtgcgg cctatattga accctgtcga agaccaaccg atggacgata tggagataat
      421 ccgaatcgcc ttcagcatta cttccaatat caggtggtgt tgaaaccggc tcctgcagat
      481 tcgcaagatc tgtatctcca aagccttgcc catttggggc tagatcccta tcaacatgat
      541 atacaatttc ttcaggatga ctgggagtct cccactttag gggcttgggg gttgggatgg
      601 gaggtccggc tagatggcat ggagatcacg caatttacct actttcagga gatcgggggg
      661 ttggagttag atccggtcac ggtggagtta acctacgggt tggaacgcat tgccatgtat
      721 ttacagcagg tcgataacgt atatgacctg atgtggacag aagacgttcg atacggcgaa
      781 ctctatcatg agtccgaagt tcagttttcc gtctataact ttgaggcggc ggatgtggat
      841 ttaattcaga ccagctttga tcgctatgaa aaggaatgcc aacagttact ggagcatcct
      901 cggggactgg tgttaccggc ctacgactgc tgtatgaaat gttcccacct ctttaacttg
      961 ttggatgcga gaggggcgat cagtgtggcc caacggacag gctatatcgg ccgggtccga
     1021 aatcttgcca ggaaatgtgc tcaagcttat cttggtcagc gggagcagat gggcttccca
     1081 ctcttacgcc acgcggcggc ttccgcccgt cctcatagaa aacaaaaggt tatttgaatc
     1141 gatgccccga tgcggggctg tggcaaatac gcttatggca acatcaagaa caacccgtaa
     1201 aaatgtcaag gccaagtctg tctcgaaatc tcagcccaag actgtacctt ttttgttaga
     1261 aattgggacg gaggaattgc cgtcagcctt tttccctaag attttggatg atttggaatt
     1321 cttggcaaaa gaaatgttta agagcagtcg attaatggat gattcattgc aaatgttgaa
     1381 tagttcactg caaacatttg gcactccccg gcgattggcg ttggttgtgg aaggagttca
     1441 actcaagcag gaaccttttc tagaagaagt tattggtccc ccaaaagccg ctgcgtatga
     1501 tcctgccggg aatccaacca aggctgctga aggctttgcc aagtcccaaa aagtgcctat
     1561 tgattggctg aagattaagg caacacccaa aggacagtat gtatatgtcg ataaaactca
     1621 accaggtcaa ccggctaaac gggtcttggc agatgcgatt cctaaactcc tggcccaatt
     1681 atattttccg aaagctatgc ggtggaatgc gtcgcgagtc aagtttgcac ggcctgttcg
     1741 atggattgta gctatgctgg gtaaagaagt tctttcgttt gagtttgcgg gcgttggatc
     1801 gaatcggcag acatggggac atcggtttcc acagaaaaag attcgaaaca tatccacagg
     1861 tttgaaaatt ccgcatgcct cccactatct ggaaacgatg aaaaaagctg ggttggtggt
     1921 ggatccagga gaacgtcgga agctaattga tgcccaagta gacaaactgg ccaaatcggc
     1981 caagggagag gtcgatcctg agacccgtca tgagctgact gagaccgccg tgtatggggt
     2041 ggagtctccg catgccatca taggggcgtt caatcctgac tttctctctg tgcccaaacc
     2101 agtgttgatt tcgtcaatga aagagcatca agggtttttc tcgttgatga caaaggatgg
     2161 cgccctcctc ccaaaattta ttgcggtgac gaatatgccg tggggaaata ccccgctcat
     2221 gaccaagggg aatgaacgag tcctggcagc ccgcctgagt gatgcgcaac atttttttaa
     2281 agaggattcg aaacgcccac tgcatgaacg agtgcccgca ttagaaggcg tcctctttca
     2341 tcacaagttg ggaacggtgg gtcaaaaagt ggatcgggtg ggtcacctga tcggatggat
     2401 ggctgagcag attggccgaa aggatctgga cgaggtgtgc cgacgcgccg ctcttctgt
//
LOCUS       DATIAL010000305         4586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1455548, whole genome shotgun sequence.
ACCESSION   DATIAL010000305 DATIAL010000000
VERSION     DATIAL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455548"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..346
                     /locus_tag="VLA60_09605"
                     /pseudo
     CDS             29..346
                     /locus_tag="VLA60_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            422..1741
                     /locus_tag="VLA60_09610"
     CDS             422..1741
                     /locus_tag="VLA60_09610"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSF09661.1"
                     /translation="MEKIEPVPPTAESVKRGSELVKEMKCIECHGLEGRGDGNAFNLK
                     DDWGFSIQPADWHKCWNFRGSRQDAYNVKNIFRTFSTGVSGTPMPSFADNTTVEDRWH
                     IANYVNHLCERDKDVDIAGGNVTDEIATALVAAKPRAIDPLTDKPKVDFVVPSKFVEG
                     ELPADEHDERWNLVDRRIVAMGGQITHKPRNFVTRIDDVWVQSLYNETHISFMFRWDD
                     RTKSVQKDSVDWEPYEVNLGDYGIEEQPPGGSKFADDPEHPESIAAKQTSYQVFNDGL
                     AFQLPIKWQELPAPRKPRYFWGDEGFPVDITKWTADGEIKAYQGAGWDVDFEDRDDFT
                     EQLKVVKAEWSNGRWTVIVTRPLKGDYEEDAYIELGKYIPINFFVWDGHNGDVGRKMA
                     VSAFYYLVLEPPIEKETYIYPTLAAIGLVLVEGWILTRRANRRKGKV"
     gene            1807..2427
                     /locus_tag="VLA60_09615"
     CDS             1807..2427
                     /locus_tag="VLA60_09615"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HSF09662.1"
                     /translation="MANIKKIANISAVVLAGGKSRRMGKDKRNLEWGGTKFLDKVCLT
                     IGGLFDEIILVTAIEDYPCGHLPVRLVTDVIPHKGSLGGIFTGIMEASYSSVFVVACD
                     MPFLNPFVISRLCALPENDVVMPRLSTGYQPLHARYSKRCSLIMEKMIQEGNLRIQSL
                     IQDPTLSIQIVEETLFVDIDPHGYSFLNINTPADYEFARKAASHLK"
     gene            2432..3511
                     /locus_tag="VLA60_09620"
     CDS             2432..3511
                     /locus_tag="VLA60_09620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HSF09663.1"
                     /translation="MPVPPQNLLIVHPGALGDGLLALPAIRVLRVMFPGHRLIWFGHK
                     ELGDVLVEAQEVYQSYSFERLEILTYRGTNDSPQEDVLSIMRRCERAIGWLEDTDGIW
                     RSWLETAGIQDCILRSPHDRTLLNHHMVDRYVEILQPWVQTTQFLHDIEINRSLTGPL
                     VLPKSHSGRLPHPLKEPLILLHAGSGSRYKCASPALWASIVKGLMMAKPKWKICLVGG
                     PADNDSLRNVQGLLTHLEYGILAGMDLLQIGQYLQHAQLFIGHDSGLSHLAASFGVPS
                     VLLFGPTDPGKWAPRGPHVAVIRKFCHCLGKAAIARCKDMPCLSFSQGEVLANVEDVL
                     NGVKASVGCPSFECVDKAFTVPCLG"
     gene            3578..>4586
                     /gene="argS"
                     /locus_tag="VLA60_09625"
     CDS             3578..>4586
                     /gene="argS"
                     /locus_tag="VLA60_09625"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HSF09664.1"
                     /translation="MEKFLQNQVIEAIAVILDKVRKSGELSLDTMPTIVVEPPKRPEW
                     GDFSSSVAMTLASQVRQSPLKVAELLATGLRAHFSDVLVRVTVAPPGFLNLTLHPLSW
                     IQVLRMIQDRGPLYGTSNIGKGKRILLEFVSANPTGPLHVGHGRGAALGQAMARLLAF
                     VGFKVSREYYINDAGRQLQLLGRSVYARYREHWGNPSSFPEDGYHGDYIKIVAESVAK
                     THGQTLLDGSSDDAETLCAQLASQMLLDRIKEDLATFGVEFDSWFSETSLHTAGLIQE
                     SLDELRQKKLLIEEDGAWWFRSSQFGDEKDRVAQKQDGSYTYLAADMAYHRQKLDRGF
                     DT"
BASE COUNT         1166 a          954 c         1218 g         1248 t
ORIGIN      
        1 aggctttgct accacgtccc aggcagaaat ggcggaagga tttgccgagg gaagtcgccc
       61 tgctccacca tctgccgaac aagttgaagc agggaagcga gtgtatttca caaagtgtgt
      121 ttggtgccat ggcgtaaacg gggccgggga cgggccagga gcagaccgac tatggccaag
      181 gccacgtaac tttaatgcag ggacatttaa aatccgtcat acggccagcg gcgaattgcc
      241 actcattgac gtagatctct ttcaaactgt gacacatgga ttaccgggat cggccatgcc
      301 ctcctgggag ggtattttga ctgaggatca acggagggat gtcctagcct tcgttaccac
      361 cgaattggtc aaggatcgta gttggcaaga taccgaattt gaagaatttc atgtgttgca
      421 attggaaaaa attgaaccgg ttcctcctac ggcagaatcg gtgaagcgtg gctccgagct
      481 tgtgaaagag atgaagtgta tcgaatgcca tggtttggag gggcgcggtg atgggaatgc
      541 gttcaacctc aaggatgact ggggattttc catacaacct gccgactggc ataaatgttg
      601 gaatttcaga ggcagccgcc aggacgccta taatgttaag aacattttta gaaccttttc
      661 aacaggggta agcggaacac ctatgccttc gtttgccgat aatacaactg ttgaagatcg
      721 gtggcacatt gctaattatg taaaccatct ctgtgaacgg gataaggatg tagatattgc
      781 cggtggtaat gtgacggatg aaatcgccac ggctttagtt gcagccaaac ctagggcgat
      841 tgatccttta acggataagc ccaaagtcga tttcgtggtt ccttcaaagt tcgttgaagg
      901 agagttacct gcagatgagc atgatgaacg gtggaaccta gttgatcgaa ggattgtggc
      961 catgggtgga cagatcacgc acaagccaag aaacttcgtt accagaattg acgatgtctg
     1021 ggttcaatca ttgtacaatg aaacccatat ttcgtttatg ttccggtggg acgaccgtac
     1081 aaagagtgtg caaaaagata gtgtagactg ggagccctat gaagttaatc ttggtgatta
     1141 tgggatagag gagcagcctc ccggaggatc gaagtttgct gatgatccag aacatcctga
     1201 atccatcgcc gccaaacaaa cttcctacca ggtgtttaat gatgggcttg ccttccaatt
     1261 accgataaaa tggcaggaat tgcctgcccc tcgaaaacct aggtatttct ggggcgatga
     1321 gggatttcct gtcgatatca cgaaatggac ggccgatgga gaaataaagg cttaccaggg
     1381 tgccggttgg gatgtggatt ttgaagatcg tgatgacttc acggaacaat taaaagtggt
     1441 gaaagccgaa tggagtaatg ggcgttggac ggttattgta acccgaccac tcaaaggtga
     1501 ctatgaagaa gatgcctaca ttgagttagg gaaatatatc cctatcaatt tctttgtttg
     1561 ggatggtcac aatggggatg tagggcgaaa gatggcagtc tccgctttct attatctggt
     1621 gttagagcca cctatcgaaa aagagacata tatttatcca acgctggccg ccattggttt
     1681 ggttcttgtg gaagggtgga ttttgacccg gcgggccaat aggcgcaagg gtaaagtcta
     1741 acaaaagttg taaacctcgg aattatccga aaaaatgaag ggggtgggca accaccccct
     1801 ttttttatgg ccaatataaa aaaaatcgca aacatttccg ctgtggtcct agctggcggg
     1861 aaaagccgga ggatgggaaa ggataaacga aaccttgaat ggggaggaac taaatttctt
     1921 gataaagttt gtcttaccat tgggggatta tttgatgaga taatattggt gactgccata
     1981 gaagattacc cttgcggaca tctccctgtc cgtttagtga ctgatgtgat ccctcataaa
     2041 ggctcattag gtggaatctt tacggggatc atggaagcct cttattcttc tgtatttgtt
     2101 gtggcttgcg atatgccttt tttaaatcct ttcgttattt ctaggctttg tgctctgcct
     2161 gaaaatgatg ttgtaatgcc taggctttcg acgggatatc agcctctcca cgcacgatat
     2221 tccaaacgat gctctctcat aatggagaag atgattcagg aagggaatct tcggattcaa
     2281 agtctgattc aagacccaac tctctccatt caaattgttg aggagactct attcgttgac
     2341 attgatccac atgggtattc ctttttgaat atcaatacac cggctgatta tgaatttgcc
     2401 agaaaagctg cttctcatct gaaataatgg tatgcctgtt ccccctcaaa atttattgat
     2461 tgtccatcct ggagcattag gtgacgggtt gttggctttg cccgctattc gggtattgcg
     2521 tgttatgttt cctgggcacc gtctgatttg gtttggccat aaggaattag gtgatgtgct
     2581 tgtggaagcg caggaagttt atcagtccta ttcttttgag aggctggaga ttttgacata
     2641 tcgagggact aatgattccc ctcaggaaga tgttctgtcg attatgcgtc gttgcgagag
     2701 agccataggt tggttggagg acacagatgg tatttggcga agttggctgg agactgcggg
     2761 aattcaagat tgtattcttc ggtcacctca tgacagaaca ttgcttaatc atcatatggt
     2821 tgatcggtat gtggaaatac tccaaccgtg ggttcagacg acacagtttc tgcatgacat
     2881 agaaataaat agaagtctta caggtcctct tgttttaccc aaatcacact cggggagact
     2941 tccccatccc ctcaaagagc ctcttatcct tctccatgct ggaagtggca gtcgatacaa
     3001 atgtgcctct ccggcattat gggcgagcat tgtcaaaggc ctgatgatgg caaaaccgaa
     3061 atggaagatt tgtcttgtag gaggacctgc agataatgac tcgcttcgaa atgtgcaagg
     3121 cctactaact caccttgaat atggcattct ggcaggaatg gatctgcttc agatcgggca
     3181 gtatttacaa catgcccaat tgtttattgg gcacgattcc ggtttgtcgc atttagctgc
     3241 aagttttggg gtgccttccg tgttgttgtt tggtcccact gaccctggaa aatgggcgcc
     3301 gcggggacct catgtagccg taatacgaaa attttgtcat tgcctaggga aggcggccat
     3361 tgcgcgttgt aaggatatgc cgtgtctttc tttttcccaa ggtgaagtcc tcgcaaatgt
     3421 tgaagacgtg ttaaatggag tgaaggcgtc cgtgggctgc ccaagcttcg agtgtgttga
     3481 taaagcgttc actgtgcctt gccttggcta gaatgctgtg ttacattcgc cgcggtcata
     3541 aaaacccata tattctgcgt agataggatg ttccaccgtg gagaaatttc tccaaaacca
     3601 agtcattgag gccatcgcgg ttatcctcga caaggtcagg aagtccggcg aattaagtct
     3661 ggacacgatg ccaaccattg tcgtggaacc tccaaagcga ccggaatggg gggatttttc
     3721 ttctagtgtc gcgatgacat tggcctcaca agttcgacaa tcgcctttga aagtagccga
     3781 actgttggcg acaggtcttc gggcccattt ttcagatgta ttggtccgcg ttaccgttgc
     3841 ccctccagga tttttgaatt tgactctcca tcccctcagt tggatccagg ttttacggat
     3901 gattcaggac aggggaccgt tgtatggcac cagcaacatc ggaaaaggga aacgtattct
     3961 cctggagttc gtgagtgcga atccgactgg gccccttcat gtcggacatg gacgaggagc
     4021 ggcattggga caggccatgg ccaggttgtt ggcatttgtc ggatttaagg tttcccgtga
     4081 atattatatt aacgatgccg gtcgtcaatt acagttgttg ggccggtccg tctatgcccg
     4141 ctatcgagag cattggggaa acccgtcttc ctttcccgag gatggctatc atggagacta
     4201 tataaaaatt gtggctgagt ctgtggcgaa gacccatggg caaaccttac tggatggctc
     4261 atcggatgat gccgaaacgc tctgtgctca attggccagt cagatgcttt tggatcgaat
     4321 taaagaagat ttggctacat ttggtgttga atttgactcc tggtttagcg agacatccct
     4381 gcatacggca ggccttatcc aagagtcatt ggacgaactt cggcaaaaga agctgctcat
     4441 agaagaggat ggtgcctggt ggtttcgttc atctcagttt ggggatgaaa aggatcgtgt
     4501 ggctcaaaaa caggatggga gctataccta cttagcggcc gatatggcct accatcgaca
     4561 aaaattagat cgtggctttg atacct
//
LOCUS       DATIAL010000306         3630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_809502, whole genome shotgun sequence.
ACCESSION   DATIAL010000306 DATIAL010000000
VERSION     DATIAL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809502"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3217)
                     /locus_tag="VLA60_09630"
     CDS             complement(<1..3217)
                     /locus_tag="VLA60_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HSF09665.1"
                     /translation="MKKIEEQGSPGGKPLIGGEGQEMDIIESDSSPLSSTERKQDIMA
                     DVIEQPKLPWTGERYVPGVIGDIELEHLHRYYLARELALGRDVLDIACGEGYGSLLLA
                     EVARSVVGVDASEEVIRYADERYQRSNVQFKHGACGRIPLPDASIDLIVSFETIEHHD
                     EHHEMIREFVRVLRPDGLVLISSPDKAEYSDKPHFVNSFHVKELYFEEFQELLLNYFG
                     HAQFYGQRVRYGSCIGPLHNSSTPPANFRLSQGRAHKFLSIPEPVYFLAIVGNGELPI
                     LPSGIFVPEVPEYWNQHMALQAGIEERDQQLGHLQSQLMNTMAEHARVLEERDQRFGN
                     LYARLLTKEEELRRMHSSLLWRNISKVRILRERLCPEGSWRGRVCASFVHWLKGSGLP
                     VTQSRQFPVGSLDRVLTSAKSLLPFSSHQKHQVVSFVFGRFGSFFQKTESYRRWKDRQ
                     LPVLHESGNLHFSHQSPLRQPVSLIQLEPGGMTTLIQSLNFTQVEQPLVSIVIPVYNH
                     LEYTVCCLASILRHAPRCSFEVIIVDDGSQDETPNILPDVQNIRYCRNETNIGFLHSC
                     NRGAALARGQYLLLLNNDTQVLEGWLDELVNTFQEQSEVGLVGSKLLYPNGVLQEAGA
                     IIKPDGSAELVGLNDDPGRPEYNVVREVDYCSGACLLIKAELFKTLGGFDDIYAPSYC
                     EDSDLAFRVRKLGMRVFYQPRSVVVHHLSVSTNDSKHAKMPLIAKNSRIFIERWSDEL
                     KKLHEVRAIAFFLPQFHPIPENDRWWGKGFTEWTNVTKARPNFQGHYQPHLPADLGFY
                     DLRMPEVREEQARLARQYGISAFCYYHYWFAGKKLLNRPFDEVLQSGSPDFPFCLCWA
                     NENWTRRWDGCEEDILIAQSHTQEDHAGFIAEIAPALLDPRYVRIHGKPLVIVYRLGQ
                     FPEPKRTADLWREYCVNAGIGEIYLAYVQCFDRMPMGDDPSLYGFDAAIEFPPHRYPV
                     QAELSQPLINPEYQGVLFDYVQTSENFIRRTWPSYKLFKGVMPSWDNTARRQDVSHVF
                     VGATPERYEYWLRQIVEQTRRFHFGDERVVFINAWNEWAEGN"
     gene            complement(3358..>3630)
                     /locus_tag="VLA60_09635"
     CDS             complement(3358..>3630)
                     /locus_tag="VLA60_09635"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HSF09666.1"
                     /translation="YLMYRDVNRSLEKGQRFHAMFRFQLPYLPTGDYSVIAAIAEGTP
                     ENHSLHHWIDDALFFRVHSSHVQRGLIGIPMLSIEFESSVMPLASS"
BASE COUNT          986 a          964 c          818 g          862 t
ORIGIN      
        1 ggttcccttc ggcccattca ttccatgcgt taataaaaac cactcgttca tcgccaaaat
       61 ggaatcgccg tgtttgttca acaatctgtc gcaaccagta ttcgtatcgt tcgggagttg
      121 cacccacaaa gacatgagag acgtcttgcc gacgcgcagt attgtcccac gaaggcatca
      181 cacctttaaa gagtttgtaa gagggccagg ttcttcgtat aaagttttcg gatgtctgga
      241 cataatcaaa caacacgccc tgatactctg gattaatcaa gggttgtgag agctcagctt
      301 gcaccggata ccgatgagga ggaaattcaa tagccgcatc aaacccatag agacttggat
      361 cgtctcccat cggcattcga tcaaagcatt gaacataggc taagtaaatt tctccaattc
      421 ccgcgttcac acagtattcc cgccacaaat ccgcagtccg tttcggctcc ggaaactggc
      481 cgaggcgata cacaatcacc aaaggctttc catgaattct gacataccgg ggatctagca
      541 gcgctggagc aatctccgca ataaagcccg catgatcctc ctgggtgtga ctttgtgcga
      601 tgaggatatc ctcctcacac ccgtcccatc gtctcgtcca gttctcattg gcccaacaga
      661 ggcagaacgg aaagtccggg gaaccggatt gcagcacttc gtcaaagggt cgattcagca
      721 gtttctttcc ggcaaaccag taatggtaat agcagaaggc cgaaatgcca tactggcgag
      781 ccaaccgggc ctgctcttct cgtacctcag gcatacgtaa atcataaaat cctaaatccg
      841 ccgggagatg gggttgataa tgaccttgga aattcggtcg cgctttcgtt acattcgtcc
      901 attccgtaaa gccttttccc caccaccgat cattttcagg aatgggatga aattgaggaa
      961 gaaaaaatgc gatcgcacga acctcatgaa gcttttttag ctcatcgctc catcgttcta
     1021 tgaaaattcg actatttttc gcaatgagag gcatcttcgc gtgcttggaa tcattggtgc
     1081 tgacgctgag atggtgaacg accacggaac gcggttgata gaagacccgc atccccaact
     1141 ttcttacacg gaaggctaaa tccgaatctt cgcaataact gggtgcatag atgtcatcaa
     1201 atccccccaa ggtcttaaat aactcagcct tgatgagcaa gcaggcccct gaacaataat
     1261 cgacctcccg caccacatta tattcaggcc tgcccggatc atcattcaac cccaccagtt
     1321 ccgctgaacc atccggctta ataatggccc cagcctcctg gagcacacca ttgggataca
     1381 gcaattttga acccacaagg ccaacttctg attgttcctg aaaggtattc accaattcat
     1441 caagccatcc ctcaagtacc tgcgtgtcat tattcagcaa cagcagatat tggccgcgag
     1501 ccaatgctgc acccctattg caagaatgga gaaatccaat attagtctca ttcctgcaat
     1561 aacgaatgtt ctgcacatcc gggaggatat tgggggtctc atcctgcgac ccatcgtcaa
     1621 cgataatcac ctcaaagcta caccgaggcg catgcctcaa aatcgaagcc aaacaacaca
     1681 cggtgtattc caggtgattg tagaccggga tcacgatgga caccaagggc tgctccactt
     1741 gggtgaagtt taaggactga atgagcgtgg tcatcccacc cggctcaagc tgaatgagag
     1801 agaccggctg acgaagagga ctctgatgag agaagtgtag atttccagat tcatgcagga
     1861 caggcaattg ccggtctttc cagcgtcgat aactttccgt cttttgaaag aatgagccga
     1921 accgtccaaa gacaaacgat acaacctgat gtttttggtg agaggaaaag ggcaataggg
     1981 attttgcact cgttaggact cgatcaagcg atccaacagg aaattgccgg ctttgagtca
     2041 caggaagacc tgagcccttc aaccaatgca caaaacttgc gcacaccctt ccacgccatg
     2101 agccctctgg acacagccgt tccctcagga tccttacttt actgatattt cgccagagca
     2161 aggatgaatg catgcggcgt aattcttcct ctttcgtaag cagtcgggca tacaggttgc
     2221 caaaacgctg gtcgcgttcc tcaaggacac gggcatgttc agccatggta ttcatcaact
     2281 ggctttgaag atgcccaagc tgttgatcgc gttcttcaat accagcctgc aaggccatgt
     2341 gttgattcca atactcggga acctcgggaa caaatatccc agaagggagg ataggtaatt
     2401 ctccgttccc gacaatggcc aggaaataga caggctcggg tatactcaaa aacttatggg
     2461 ctcgcccttg ggataatcga aaattcgctg gaggcgtgga ggagttatgg agcggaccaa
     2521 tacatgatcc ataccggact cgttggccat aaaactgcgc atgcccgaaa taatttaaca
     2581 aaagttcctg aaactcttcg aagtagagtt ctttcacatg aaaggaattg acgaagtgtg
     2641 gtttatcgga atattcggcc ttatcagggc tggagattaa gaccagaccg tccgggcgca
     2701 aaactcgaac aaattcccgt atcatttcat gatgctcatc atggtgttcg atcgtttcaa
     2761 aactcacaat aaggtctatg ctggcatcag ggaggggaat gcgaccacag gctccgtgct
     2821 taaactggac gttcgaacgc tgatagcgtt catctgcata gcgaattacc tcttccgagg
     2881 catccacacc cacaacactc cgggcaacct cggctaacag cagggaacca tatccctccc
     2941 cgcatgcaat atcgagcaca tcccttccta acgccagttc gcgagcgagg taatagcggt
     3001 gcaaatgctc tagctcaata tctccaatga ctcccggcac ataacgttcg cctgtccacg
     3061 gaagctttgg ctgttctatc acatccgcca tgatatcctg ctttctttca gtagaagaca
     3121 gagggcttga atcggattca atgatatcca tttcctggcc ttcgccccct attaatggtt
     3181 tcccccctgg tgaaccttgc tcctcaattt ttttcatgat ttggctctat gtaaaattga
     3241 ccgccaccaa gccgaggtta caatgacgtc tgcactctct aaaaccaccg aaatgtgctt
     3301 tcggctgtgc gcaatatgga acacctctaa atttgacccc atatcactga gctcttctca
     3361 cgatgaggcc aagggcatca ctgaggattc aaattcaatc gataacattg gaatcccaat
     3421 gagcccccgt tggacatggc ttgagtggac tcggaaaaac agggcatcgt caatccaatg
     3481 atgaagagaa tgattctctg gtgtcccctc ggctatcgcc gcaatcaccg aataatcgcc
     3541 ggttgggaga tacggtaact gaaaacgaaa catcgcgtga aatcgctgcc ccttttcaag
     3601 ggagcggttc acgtcacgat acatcaaata
//
LOCUS       DATIAL010000307         7257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_57379, whole genome shotgun sequence.
ACCESSION   DATIAL010000307 DATIAL010000000
VERSION     DATIAL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57379"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VLA60_09640"
     CDS             complement(<1..1100)
                     /locus_tag="VLA60_09640"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HSF09667.1"
                     /translation="MNDGVTRRQVLATGAALAAGTLSAQLGHGESVDPSRSRTDQDQT
                     AKISNEKFSRYSRYRPSFGGPPDSDEYLGKLVPGLRKSGLPPVPIQTPDLEVLPWKMV
                     NGVKEFELRCTPVKREFLPGKFMNVWGFNDSMPGPTMEAVQGDRVRIIVQNELPEPTS
                     VHWHGLELPVEFDGVPGVTQHLIPPGGTYVYEYDLHQTGTFFYHSHVAMQEAFSMIGF
                     FIIHPRVAYDPPVDRDFGLIFQNFFIPPNASTPDSMGMDWNWHTINGRSGPYTTPLVC
                     KHGERVRIRLIDFSPMQHHPIHIHGHTFWLTGTEGGRIPSSSWIPRNTTLVGVAMAQD
                     FEFIAFNPGDWTFHCHMVHHMMNHMVRQVGPR"
     gene            complement(1147..2595)
                     /locus_tag="VLA60_09645"
     CDS             complement(1147..2595)
                     /locus_tag="VLA60_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF09668.1"
                     /translation="MIKIFMVGLGWPIILVFGWGLSGCVIPFHDNQMDELPSPNMYPQ
                     EGIPAPFPDLQNENHQDNGLTEFPTQADDEHDDGKTLSLQRLKKLARKNNPTLIQAWV
                     LVEGERAKALQAGLYPNPVGGYSGDQINVKNTIGEFQGGFIQQEFVTAGKLELSREKY
                     LARASAAEYQALAQEYRVMNGIAIQYYRLLGFQERVDIQRELLKSWQDQLLTVKEMFN
                     VGQANEADLRQARVQLQQQQLSVLMAENEVQMERERLMALVGTSLPGQPVSGMLAEDL
                     PPIRFEEALKRLRQESPELALAHANVRSDQIMVQREQAEPIPNITVRGSAGRNYVETQ
                     TVYGIQAFIKIPIFDWNQGTIQQARADLRRQEAQVTLTELRLRRSLAEQFQQYLTALQ
                     HITSYRDLLLPESEERYRIQLQSYQSARETWPEVLQAQRDFFTLRMEYINQLIAWRTA
                     RVAIEGLLLIDGLQAPQGVTPPGHLDANPKPR"
     gene            complement(2877..3227)
                     /locus_tag="VLA60_09650"
     CDS             complement(2877..3227)
                     /locus_tag="VLA60_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HSF09669.1"
                     /translation="MAHQHQPCVDACVRCAEECEGCANQCLGDMPECARLCIDCADLC
                     WSCTAFMSRDSQFSAQVCDLCADICDACAQECEKHQNEHCRRCAEACRKCAEECRKMG
                     VNAGAKKQPVGAGR"
     gene            complement(3519..3848)
                     /locus_tag="VLA60_09655"
     CDS             complement(3519..3848)
                     /locus_tag="VLA60_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09670.1"
                     /translation="MTIQLNWKLLVGATLIALTVGCASSPPRDLAQRNDHAGLTAWYQ
                     EEARDLRMRAEKMRQMGKYYEIYTPKQGQQPNLVQHCRNLVDKYIQAAEELDALAKLH
                     AEEKKTP"
     gene            complement(4120..>7257)
                     /locus_tag="VLA60_09660"
     CDS             complement(4120..>7257)
                     /locus_tag="VLA60_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSF09671.1"
                     /translation="RNRFIVFLILFGLASAGFWAMKQIPVDAVPDLSDTQVIIYTKWP
                     GRSPDLVEDQITYPIVTALLSAPNVTVVRGFSDFGFSYVYVLFKDGTDMYWGRSRVLE
                     YMNQLAGRLPNGVTPQLGPDATAVGWVFEYALIDETGQQDLASLRSFQDWYLRYWLLA
                     VDGVAEVASVGGFVRQYQVNLDPTKVQAYNLSLPHIIETIRMSNNDFGGRVVEFSGVE
                     YMIRGRGYLSSVADIEQIAMGVSPHGTPILLRDVATVTLGPDMRRGLVELDGQGEVVG
                     AIVLMRFGENALTVTERIKAKLNELESSMPPGVKVVTTYDRSGLIHESIATASENLIE
                     ELIVVSVLIIGFLLHFRSALLPILTLPLAVLLAFIPMYLMNIGLNIMSIGGIIVAIGD
                     MVDAAIVMVDNAHKRLEEWVKDGQKGDRTQVLIDSAKEVGPAIFASLLVIAIAFMPVF
                     TLEGQEGRLFKPLAFTKNFAIAMSAILAITLIPALLPLLVRGRIIPERRHPVSLGLQK
                     LYAPLLHVALRYRTLMIVLAILLTITVFPAFQRMGSEFMPPLYEGTILYMPNTLPGIS
                     VTQASMLLQEMNQKLKAFPEVERVFGKAGRADTSTDPAPFSMMETVVTLKPKDEWRAG
                     VTYESLVDEMDGALQFPGVTNTWTMPIKGRLDMLTTGVRTPVGIKIFGPDLQEIEKIG
                     KHLETVLKDVPGTRSVYAERVSGGYFLDFDIKREEIARYGLNVTDVGRIIESAIGGEN
                     IDTTIEGRERYPVNVRYLRELRDTPEKLRRVLVETPTKAQVPLAQLASLRFVSGPPMI
                     RDEDGMLAGYVYVDMTGRDVGSYVEDLKNVVQAKVTLPQGYRLAWSGQYEFMERVRER
                     LVIFVPLTISIIFILFYFAFQSMAKTLMVMLGVPLSLVGAVWYMVWLDYNMSIAVWVG
                     IIALAGVAAETSAVMVTYLDEALKRRRKANRLQSLKDLIEGVQQGAVERIRPMAMAGL
                     ANILGLIPVMWATGTGADVMKRLAAPMVGGVASAMLLTLFVIPAIYTIWFWHTDLKKA
                     SHKTAIELGRDPATE"
BASE COUNT         1742 a         1957 c         1830 g         1728 t
ORIGIN      
        1 atgcgaggac cgacttgtcg caccatatgg ttcatcatgt ggtgcaccat gtggcaatga
       61 aaggtccaat cgccgggatt gaaggcaatg aattcgaaat cctgagccat cgccactccc
      121 accaatgtcg tattccgagg aatccaggag ctggatggaa tgcgtccccc ttccgttccc
      181 gtcaaccaaa aggtatgccc atggatatga atcgggtgat gctgcatcgg gctgaaatcg
      241 atcaatcgaa tgcggactcg ttccccatgc ttgcacacca ggggagtggt ataggggccg
      301 ctacgtccat taatggtatg ccagttccag tccatgccca tggaatccgg cgtcgaggca
      361 ttgggcggga tgaaaaagtt ctgaaaaata agtccgaaat cccggtcaac gggcggatca
      421 taggccacgc gcggatggat aataaaaaat ccgatcatgc taaatgcttc ctgcatggct
      481 acatggctat gataaaaaaa agttccggtc tggtgcaggt cgtactcata cacataggta
      541 ccccctggtg gaatcagatg ttgtgtgaca cccgggacac cgtcaaattc caccggaagt
      601 tccaggccat gccaatgcac ggatgttggt tccggtagtt cattttggac aataatgcga
      661 acccgatccc cctggacggc ttccatcgtg ggccccggca tgctgtcgtt aaacccccaa
      721 acattcataa atttccccgg aagaaattca cgcttgaccg gcgtgcatcg aagctcgaat
      781 tccttgacgc cattgaccat tttccaaggc agtacctcca gatcgggtgt ctgaatcggc
      841 accgggggca gcccggattt gcgtaaaccg ggcacaagct tccccagata ttcatccgaa
      901 tccggggggc cgccaaaact ggggcgatag cgtgaataac gggaaaactt ttcattggaa
      961 attttggctg tctggtcttg gtctgtgcgg gaccgggagg gatcgactga ctcaccatga
     1021 cccaattggg ccgacaaggt tcctgcggcc agtgcagccc cggttgccaa aacctgtcga
     1081 cgtgtcacgc catcgttcat cgtacaatct ccatatagga aaaatgtgtc ttgtggaaat
     1141 tgacgcttat ctgggtttgg gattcgcatc caggtgccca ggcggcgtca caccctgtgg
     1201 ggcttgaagt ccatcaatga gcaggagtcc ctcaattgcc accctggccg ttcgccaggc
     1261 aattaactga ttgatatatt ccatccgaag cgtgaagaaa tcgcgttgag cttgcagaac
     1321 ctccggccag gtttcacggg cggattgata actctgcaat tgaatacgat accgttcctc
     1381 tgattcgggc aacagaagat cccgataact cgtgatatgt tgaagggcgg tgagatactg
     1441 ttgaaactgt tcggctaaac ttcggcgcaa tcgaagctcc gtaagtgtga cttgggcctc
     1501 ttgtcggcgg agatcggccc gggcctgttg aatcgttccc tggttccaat caaaaatcgg
     1561 aattttgata aacgcctgaa tgccatagac cgtctgtgtt tcgacataat tccggccggc
     1621 cgaacctcga acggttatgt tgggaatagg ttccgcctgt tcccgctgaa ccataatttg
     1681 atccgatcgc acattggcat gagccaaagc cagttcaggg ctttcctgcc gtaacctctt
     1741 gagggcctcc tcaaatcgga tgggaggcag atcttctgcc agcatccccg aaaccggttg
     1801 accgggaagg gacgtcccca cgagagccat taagcgctct ctttccattt ggacttcatt
     1861 ttctgccatg agcacggaca attgttgctg ttgaagttgc actcgggcct ggcgcagatc
     1921 agcctcattg gcttgaccga cattaaacat ttccttgacg gtcaagagct gatcctgcca
     1981 acttttgaga agctcccgtt ggatgtctac ccgctcctga aatcccagaa gacgataata
     2041 ttggatggca atcccattca tgacacgata ttcctgcgca agggcctgat attcagcggc
     2101 ggaagctcgt gctaaatatt tctcacgact caattcgagt tttcccgccg tgacaaactc
     2161 ttgttgaata aaccctccct gaaattctcc aatggtgttc ttgacgttga tttgatctcc
     2221 tgaatagccc ccgaccggat tggggtagag gcccgcctga agagccttgg cccgttctcc
     2281 ctcgactaga acccaggcct ggatgagagt aggattattt ttccgagcga gtttcttgag
     2341 ccgctgaagt gaaagggttt tcccgtcgtc atgctcgtca tccgcctggg ttggaaattc
     2401 tgttagtcca ttgtcctggt gattctcatt ttggagatcg ggaaatggag ccgggattcc
     2461 ctcctgggga tacatgttcg ggctaggcag ttcatccatc tgattatcat ggaaagggat
     2521 aacacatccg gataatcccc atccgaatac caatataatt ggccacccta acccgaccat
     2581 gaagatcttg atcattttta tatcctatga aatccgtggt gtcttttttg gacagtctag
     2641 aagattttct caatggtcga actggataat tttaaaaata tggcaaagga aatattcaat
     2701 cgaatcttcg atggaatcag gtagatcccc ttcaccgttt tcggcccatc cctggtaatg
     2761 aatggacttc tggacaggct cctgtccggg taggctaaga ttgaatggaa gaaagggaga
     2821 tcgacctcac cgggcatgcc tctgggaggc cttgcccgat gagtagtcaa ctaaggctat
     2881 cgcccggcac ccacaggttg tttttttgct cctgcattca cacccatttt gcggcattct
     2941 tccgcgcact tgcgacaggc ttcggcgcat cttcggcagt gttcattttg atgcttctcg
     3001 cactcttggg cgcaggcgtc acagatatcg gcacagaggt cacagacctg agcactgaat
     3061 tgagagtcac ggctcataaa ggccgtgcag gaccagcaga ggtctgcaca atcgatacaa
     3121 agtctggcac actccggcat gtcacccaag cattggtttg cgcaaccttc acactcctca
     3181 gcacatcgca cacaggcatc cacacaaggt tgatgttgat gagccattgg aaaatcctcc
     3241 tttattgaga gtacaggcaa atctgaccat taaaaacatt atgctgaaag ttaaaataac
     3301 tctcccagtc taaaagcgta cgctcaacga aaaaactgtc gaaaacccta gtttgggatt
     3361 atagtgggat tatgaaggaa ttgattatag aagaggacga tcactaaccc ataactttga
     3421 atctcgaata cacaattggt ttttgctttt tcaacctcgg tgtgaaagca actctggagg
     3481 ctgtctccac accgggttac cctcttggta ggaaggaatt atggagtttt cttctcttcg
     3541 gcatgaagtt tagccaaagc gtctaattct tccgcggctt ggatgtattt gtccaccaaa
     3601 ttccgacaat gctggacaag gtttggttgt tggccttgtt tgggagtgta tatttcgtag
     3661 tatttcccca tttgacgcat cttctctgcc cgcatgcgaa ggtcacgcgc ttcttcctga
     3721 taccaggcag ttaaccccgc atgatcattt cgttgcgcca ggtccctggg cggacttgaa
     3781 gcgcacccca ctgttaacgc aatcaatgtg gctcccacca acaatttcca gtttaattga
     3841 attgtcatga tctcctcctt gtgaatggta gtctcgagga aactgagata ttccagcgca
     3901 gctattcaat cccaaagcaa tgagatagga accgagtcaa tggtgacgtc aaaagtattc
     3961 ctccttattt tcttgattga gggaatcatc ggtcgaatgt cctttctttg ccaacgtaga
     4021 ttttgtatgc cggaacgttc ttcccaactt gctaggtcgt gcctaccgaa gtatccgacc
     4081 tctttttttt gcccctcgca tttaaaacaa ccactcaact cattccgtcg ccggatctcg
     4141 gcccagttct attgctgtct tgtgggaggc ctttttaagg tctgtatgcc aaaaccaaat
     4201 cgtgtaaatg gccggaatca cgaacagcgt gagaagcatg gctgatgcga cgccgccgac
     4261 catcggcgcc gccagacgct tcatgacatc cgctcccgtg ccggtggccc acatcaccgg
     4321 gatcaacccc agaatattgg ccaggcccgc catggccatg ggccgaattc gctcaaccgc
     4381 tccttgctgg acaccctcga tcagatcctt taaggattgc aggcgattgg ccttgcgacg
     4441 acgtttgagc gcctcgtcga gataggtcac catgaccgca ctggtttcag cggcgacgcc
     4501 ggccaacgcg atgatcccca cccagacggc gatactcata ttgtagtcca accagaccat
     4561 gtaccagact gcccccacga gggagagtgg cacacccagc atgaccatga gggttttcgc
     4621 catggattga aaggcaaaat agaacagaat gaaaatgatg gaaatggtca gagggacaaa
     4681 aataacgagc cgttcccgaa cccgctccat aaattcatac tgccctgacc atgccagtcg
     4741 atatccttga ggaagtgtca ccttggcctg tacgacgttt ttcaaatcct ccacgtaact
     4801 ccccacatcc cgtccggtca tgtccacata cacatacccg gccagcatgc catcctcatc
     4861 gcggatcatc ggggggccgc tcacaaatcg taaggaggcc aattgcgcaa gagggacttg
     4921 ggctttggtc ggggtttcca ccaacacccg tcgtaatttt tccggggtat cccgaagttc
     4981 tctcaaatat cggacattga ccggataccg ctctcgtcct tcaatggtgg tgtcaatgtt
     5041 ttcccctccg atggcggatt cgatgatccg gccgacatcc gtgacattga gtccgtaccg
     5101 tgcaatttct tctcgtttaa tatcgaaatc taaaaagtac ccacccgaaa ctcgctccgc
     5161 atagacactc cgtgtgccgg gcacgtcttt caataccgtc tcgagatgct tgccgatctt
     5221 ttcaatttcc tggagatccg gaccgaaaat tttgatcccg accggggtgc gcactccggt
     5281 ggtcagcata tccagccggc ctttgatggg catggtccat gtgttggtga cgccagggaa
     5341 ttgcaaagcc ccatccattt catccacaag actttcatag gtgacgcccg ctctccactc
     5401 atctttcggt ttaagtgtga ccacggtttc catcatgctg aacggggccg gatcggtcga
     5461 ggtatcggca cgcccggcct ttccgaacac tcgttccact tcgggaaagg ctttcagttt
     5521 ctgattcatc tcttggagca acatcgaggc ctgggtcacg gaaattcccg gaagcgtgtt
     5581 cggcatatag agaatcgtgc cttcgtagag tgggggcatg aattctgacc ccatccgttg
     5641 aaaggcgggg aagacggtga tcgtcagcag gatagccagg acaatcatga gggtccgata
     5701 tcgtaaggcg acatgtaaca aaggcgcata cagcttctgt aaccccagac ttaccggatg
     5761 ccggcgctcg ggaatgatcc ggcctcgtac cagcaacggc agcaacgcgg gaatcagcgt
     5821 gatggccagg atggcgctca tggctatggc aaagttctta gtgaacgcga ggggtttgaa
     5881 taaccgtccc tcctggcctt ccagagtgaa caccggcatg aaggcaattg caatcaccag
     5941 cagggaggcg aagatggccg gccccacctc tttggcggaa tcgatcagga cctgggtccg
     6001 gtcccctttc tgtccgtcct taacccattc ttctaatcgc ttatgggcgt tatcgaccat
     6061 gacaatagcg gcatcgacca tatccccgat ggccacgata atccccccaa tggacatgat
     6121 attcaggccg atgttcatca aatacatagg aatgaaggcc aacagcaccg ccaatggaag
     6181 tgtgagaatg gggagcaggg ctgatcgaaa atgaagtaaa aacccaataa taagcacact
     6241 gaccacaatg agttcttcaa tgagattttc ggacgctgtc gcgatggatt catgaatgag
     6301 cccgctccga tcgtatgtcg tcacgacctt caccccagga ggcatggatg actccaactc
     6361 gttgagtttg gccttgatcc gttcggtgac ggtgagcgca ttttccccga atcgcatgag
     6421 gacgatggct cccacgactt cgccttgacc gtccagttca accaatcccc gtcgcatatc
     6481 cggtcccaag gtgacggtgg ccacatcgcg taataggatg ggagtcccat ggggactgac
     6541 ccccatggca atctgttcga tgtcggccac cgacgataaa tagccccgcc cccggatcat
     6601 atattccacc cctgaaaact ccacgacccg tcccccgaaa tcgttgttgc tcattcggat
     6661 ggtttcgatg atatgcggga gagaaaggtt gtaggcctgg accttggtgg gatccagatt
     6721 tacctggtat tgccggacaa agcctccgac gctggcgacc tcggccacgc catcgacagc
     6781 caagagccaa taacgaaggt accagtcttg aaacgatcgg agtgacgcca aatcctgctg
     6841 gccggtttcg tcgatcaagg catattcaaa gacccagccg acggcggtag cgtccgggcc
     6901 caattggggg gtgacaccgt tcggtagtcg gcccgccagt tggttcatat attccaggac
     6961 ccggctgcgg ccccaataca tatccgtgcc atccttgaac aagacataga catacgaaaa
     7021 accaaagtcg gagaagcccc tcaccacggt gacattcggt gcggaaagga gagcggtcac
     7081 gatgggatag gtaatctgat cctcaaccag gtccggcgaa cggcccggcc acttggtata
     7141 gatgatcacc tgggtatcgg aaagatcggg gaccgcatcg acgggaatct gcttcatggc
     7201 ccagaatccg gccgaggcca acccgaagag gatgagaaag acgatgaagc gattccg
//
LOCUS       DATIAL010000308         6091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1455901, whole genome shotgun sequence.
ACCESSION   DATIAL010000308 DATIAL010000000
VERSION     DATIAL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455901"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1778
                     /gene="ngg"
                     /locus_tag="VLA60_09665"
     CDS             <1..1778
                     /gene="ngg"
                     /locus_tag="VLA60_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="HSF09672.1"
                     /translation="KDCDVLKAPQALHMTSPRQRPIGPTVKNWEVGPATYQGRRVKPK
                     VVLDCGWGRLIFAHTFTGPEEVASTLMQERPGHRDIAFYLRDPHVVLALRPHKLFLDP
                     SHTFRLWLGDYQSVANQQSGMVITPIQSEADVRALNRIYAARGMVTADTDLLMRHRRS
                     QKLCYLLAKDQFTTEVIGVILAVNHRLIFDDPEKGSSIWSLAVDPQAHHPGVGEALIR
                     HAAERMKARGCNYVDLSVLHDNHEAIALYRKLGFGRIPVFAVKTRNIINEKLFAGPSP
                     DQRLNPYATIIVNEARRRGIDVDVLDAENGYFRLTFGGRSITCRESLSELTSAIAMSR
                     CADKTLTRRVLQEAGLSVPAQMVANGRKRNGEFLEQYRSIVVKPATGEQGAGVSVDVR
                     RVKEMESAITLAGKSGGHVILEQYVKGEDLRIVLINYEVVAAAVRRPPRITGTGRHTI
                     AELIDRQSQRRARLTGGESRIPLDGETKRCIKEGGYTLAEVLPKGKTITVRKTANLHT
                     GGTIHDVTGKLHPDLATAAVIGAKALAIPVVGFDFIVTTVSKPHYVIIEANERPGLAN
                     HEPQPTAERFIDLLFPQTKTPTKLK"
     gene            1804..3012
                     /locus_tag="VLA60_09670"
     CDS             1804..3012
                     /locus_tag="VLA60_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant NAGGN system M42 family
                     peptidase"
                     /protein_id="HSF09673.1"
                     /translation="MIGSKIDTQYLLHILQKLLAIPSPTGYTDQIVHAVSNELKQLGI
                     PFELTRRGAIRATMAGKVSTPDRAIVAHLDTLGALVKWLKNNGRLAIVPIGTWSSRFA
                     EGARVTVFSDTSMHRGTILPLKASGHTYGDEIDTQPVSWKNLEIRLDETVTDVNGRIL
                     PLSTDQDLQRLGIHIGDFIAIDPSPEIGPNGFINSRHLDDKAGVATILAAAKSILESG
                     AMLPVDCHLLFTISEEVGSGASAILHGDVAEMITIDNGTPAPFQASSEFGATIAMADS
                     AGPFDYHLVRRLIALCVDHDIPFQRDIFCDYRSDSASAVEAGNDIRTALLTFGVDSSH
                     GYERTHLSGLEAIAKLLVVYMQNEPVFWRDRDTLGSDNDFPTQPIEETSFPHTLEDMV
                     RRHGEPQDDP"
     gene            3094..4911
                     /locus_tag="VLA60_09675"
     CDS             3094..4911
                     /locus_tag="VLA60_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HSF09674.1"
                     /translation="MSSHPPASTFPPANTTHPQLTLRAIATGMVLGALLTPCNIYSGL
                     KIGWTFNMSITAALLSYAFWKIFETTAQTERWGLLENNINQTTASSAASIISSGLVAP
                     IPALAILTGEQLPWSLLSVWVFSVSLIGIVVAVGLRQQMLLRDRLPFPAGVATAETVR
                     EIYGKGGEALARVKVLLTAGGIAGIMKIFNEAILTIPKMATGLAIPLKGALNKAGLST
                     VSLSNLGFVLDPSLLMVGFGAIIGLRAGLSLLIGAILAWGIIGPWVLTQGWIPAEHLT
                     PDGYWFGPLVEWLLWPGVTLMVMASLTSFACSWGRMVTGTILSRKTRASLLTPNDPYV
                     IPRQWFVIGLCISLVFAVMTQITLFNISMGIGILAVLLSFVLAIVAGRVSGETGITPI
                     GAMGKVTQLTFGFLIPGNVTTNLMAANVTGGAAGQCADLLHDLKTGLLLGASPRFQAL
                     AQIFGVLTGSLVGSAVYLVLIPDPQSMLLTIEWPAPAVATWKAVAEVFQLGTEAIPPG
                     SLLAMSIAGLLGVGMVVLDQSVPPSMSRWIPSASTMGLAFVIPAWNSLSLFLGALLGA
                     CLMRYAKTWAERFVMALAAGFVAGESLAGVASVLVKILF"
     gene            complement(5059..5250)
                     /locus_tag="VLA60_09680"
     CDS             complement(5059..5250)
                     /locus_tag="VLA60_09680"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSF09675.1"
                     /translation="MKRKRQLHDGIAGALITAGVALGYYVNPLWLLLPGILGVTLLQS
                     GFTGFFPVYFILDRTCPEE"
     gene            5613..>6091
                     /locus_tag="VLA60_09685"
     CDS             5613..>6091
                     /locus_tag="VLA60_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09676.1"
                     /translation="MPFGYYHQLNAKAKRIYRASDKVSDFQLPNVSVVRPVLRKIFEA
                     LQAESHLRTQKWTQRLLNRLTSQLHIRPIRFELLDIRPSNPRMELYGLYYPMEGRRIP
                     RIQVWMRTAKRHQVVAFRTFLRTLLHELCHHLDYDCLGLKDSFHTKGFYGREASLAS"
BASE COUNT         1317 a         1584 c         1618 g         1572 t
ORIGIN      
        1 ctaaggattg cgatgtgttg aaggccccgc aagctctcca catgacgagc ccccgtcagc
       61 gaccgatcgg gccgaccgtc aaaaactggg aagttgggcc ggcgacctac cagggccggc
      121 gagtgaagcc caaagtggtc ctggattgcg gctgggggcg gcttattttt gcccacacct
      181 ttacaggccc cgaagaggtg gcttccactc tgatgcagga acgtcccggg caccgggata
      241 ttgcctttta tttacgggat cctcatgtgg tcctggccct gcgcccgcac aagctgtttc
      301 tggatccatc gcatacgttt cgattgtggt tgggtgatta tcaatccgtt gccaatcagc
      361 aatcaggcat ggtgatcact cccatccagt cggaggccga tgtcagggct ttgaatcgga
      421 tctatgcggc acggggcatg gtcacggccg acacggatct ccttatgagg catcgccgct
      481 cccaaaaact ctgctatctt ctcgcgaaag accagttcac cactgaagtg atcggcgtca
      541 tcctagctgt gaatcatcgc ctgatttttg acgaccccga aaaaggcagc agcatctgga
      601 gtctggccgt tgaccctcaa gcccaccatc ccggagtggg ggaagcgttg attcgtcatg
      661 cggccgaacg gatgaaggcc cgggggtgca actatgtgga tctctcggtg ctgcatgaca
      721 accatgaagc cattgcgctg tatcggaaac tgggatttgg acgaattcct gtgtttgcgg
      781 tgaaaacgcg aaatatcatt aatgaaaaat tgtttgcagg accttcaccc gatcagcgac
      841 ttaaccccta tgccaccatt attgtcaatg aggccagacg ccgaggcatc gacgtcgatg
      901 tcctggatgc cgagaatggc tacttccgct tgacgtttgg gggccggtcc attacctgtc
      961 gagaatcgtt gtccgaattg acctcggcga ttgcgatgag ccgttgtgcg gataagaccc
     1021 tcacccggcg cgtgttacag gaagccggcc tatctgttcc agctcagatg gtggccaatg
     1081 ggcggaagcg caatggtgaa tttcttgaac agtatcgatc cattgttgtc aaaccggcga
     1141 cgggggaaca gggagctggc gtgagtgtgg atgtgcgtag ggtcaaggaa atggagtccg
     1201 caatcaccct tgccggcaaa tctggtggtc acgtcattct ggaacaatat gtaaagggcg
     1261 aggacttacg cattgtgttg atcaattatg aagtggtggc cgctgccgtt cgacgaccac
     1321 cacggatcac ggggacagga cgtcatacga ttgccgaatt gattgatcgg cagagtcagc
     1381 gacgggcacg attgaccggc ggcgagagtc gcattcctct ggacggagaa accaagcggt
     1441 gtatcaagga aggcggctat actctcgcag aggtcttgcc aaaaggcaaa accatcacgg
     1501 tgcggaagac ggccaatctg cataccggag gaacgatcca tgacgtgaca ggaaaacttc
     1561 atcctgattt agccactgcg gcggtaattg gtgccaaagc gctggctatt ccggtggtgg
     1621 gattcgattt tatcgtcacg acggtttcca agcctcacta tgtcattatt gaagccaatg
     1681 agcgacccgg tcttgccaat catgaaccgc aaccgacggc ggaacgattt attgatctgt
     1741 tgtttcctca aaccaagacg cccacaaaat tgaagtagtc ctttgcgggt gataggggat
     1801 tgtatgatcg gttcaaaaat tgacactcag tatctcctgc atattctcca aaaactattg
     1861 gcgattccga gtccgaccgg ctataccgat caaatcgtcc atgcggtgtc gaatgaactg
     1921 aaacaattag ggattccctt tgaactcacg cggcgcggag ccattcgagc caccatggca
     1981 ggaaaagtca gtacgcctga tcgagccatt gtggctcacc tggatacgct gggggctctg
     2041 gtcaaatggc tcaaaaacaa tggccggttg gccattgtgc caatcggaac ctggtccagc
     2101 cgatttgctg agggggcccg tgtgacggtc ttttccgata caagtatgca tcggggcacc
     2161 atcctgcccc tgaaagcttc tggccacacc tatggtgacg aaattgatac gcaaccggtg
     2221 tcgtggaaaa atctggaaat tcgcctcgat gaaactgtga cggacgtcaa tggacgtatc
     2281 ctacccttat cgaccgatca ggatctccag cgcctcggga ttcatattgg tgatttcatt
     2341 gccattgacc cctcgcccga aatcggaccc aatggattta tcaactcgcg gcatttggac
     2401 gataaagccg gagtggccac catcctggct gctgccaaat ccattcttga gtcaggggcc
     2461 atgcttcctg tggattgcca tttactgttt acgatatccg aagaggttgg gtcaggggcc
     2521 tcggcgatct tacatgggga tgttgcagaa atgatcacga ttgataacgg cactcccgcg
     2581 ccgtttcaag cctcgagcga attcggggcg acgattgcca tggccgattc agccgggcct
     2641 tttgattatc acctggtcag gcggctcatc gccttatgtg tggatcatga cattccgttt
     2701 caacgcgaca ttttttgcga ctacagaagt gacagtgcat cggccgtaga ggccgggaac
     2761 gacattcgga ccgctctcct tacgtttggg gtcgattctt ctcatggtta tgagcgaact
     2821 catctgagtg gactcgaggc cattgcgaag ttactggtcg tgtatatgca aaatgaaccg
     2881 gtgttttgga gagatcgcga tacgttaggg tccgataatg atttccccac tcaaccgatt
     2941 gaagaaacgt cttttcccca cacactggag gacatggtcc gacgccacgg tgaaccccaa
     3001 gatgacccat agcgcttctc tgctgaggac agacgacagg atgttcaatg cactcccatt
     3061 gcggatgttc tcctcattac cctgtctggc ttgatgtctt ctcatccacc cgcatctacc
     3121 tttcccccag ccaatacgac ccatccacag ttgacgcttc gtgccattgc caccgggatg
     3181 gtcttaggtg cgctcctcac tccctgcaat atctatagcg ggctaaagat tggctggaca
     3241 tttaatatgt ccattacggc agccttgctc agttatgcgt tttggaaaat attcgaaacg
     3301 accgcccaaa cggagcgctg gggtcttctc gaaaataata tcaatcaaac cactgcctct
     3361 tcggctgcgt ccattatctc gtcagggctg gtggccccga ttcccgcctt ggccatattg
     3421 actggcgagc aattgccttg gtctctgctc tctgtatggg tcttttccgt cagcctgatt
     3481 ggaatcgtgg tcgcggtcgg tctccggcaa cagatgcttc ttcgtgaccg attgccgttt
     3541 cccgccggtg ttgccacagc ggaaactgtg agggaaatct atgggaaagg gggggaagcc
     3601 ctggctcgcg tgaaagttct cctcacggct ggtgggatcg ccgggatcat gaaaattttc
     3661 aacgaagcta ttctgaccat ccccaagatg gccacgggcc tggccattcc actgaagggg
     3721 gcgctcaaca aggccgggtt atccactgtg tccctttcca accttgggtt cgttctcgat
     3781 ccctcgcttc tgatggtggg ctttggggcc attatcgggc ttcgcgccgg actctccctg
     3841 ctcattggag cgatccttgc ctggggcatt attggtcctt gggtcctcac gcaaggctgg
     3901 attcctgcgg aacatctgac gccggacggc tattggttcg gacccctggt ggaatggttg
     3961 ctctggccgg gggtgaccct gatggtcatg gcctcgctca cgtcgtttgc ctgctcctgg
     4021 gggagaatgg tgaccggcac catactttct cgaaaaaccc gagcatccct tttaaccccg
     4081 aatgatccct atgtcattcc acgtcaatgg tttgtgattg gactgtgtat ttctttggtc
     4141 ttcgcggtga tgacccagat aacgttattc aacatttcaa tgggtattgg gatcctcgcg
     4201 gtccttctct cctttgtatt ggccatcgtg gcgggacgag tctcggggga aaccgggatt
     4261 acgccaatcg gcgccatggg gaaagttaca caacttacgt ttggtttcct cattccgggt
     4321 aatgtcacga ccaatcttat ggcggccaat gtcacagggg gagcggccgg tcaatgcgca
     4381 gacctcctcc acgacctgaa gaccggtctg cttcttggtg cgtcgccgcg ctttcaagct
     4441 ctggctcaaa ttttcggagt gttaaccggc tcacttgtgg gcagcgcggt gtatctggtg
     4501 ctcattcctg atcctcaatc catgttgtta actatcgaat ggcctgcgcc tgccgtggca
     4561 acctggaagg ctgttgcgga agtctttcag ctcggtacag aggctattcc tccgggaagt
     4621 ctgttggcca tgagcattgc gggtttacta ggcgtgggga tggtggttct cgatcaatct
     4681 gtcccaccat ccatgagccg atggatcccc agcgcctcga ctatgggact ggcgtttgtc
     4741 attcctgcct ggaactcgct gtctctcttt cttggtgctc ttcttggagc ctgtttaatg
     4801 aggtatgcga agacctgggc tgaacgattt gtgatggcac tggccgccgg gttcgtggct
     4861 ggtgaaagcc tcgcaggtgt cgcgagtgtt cttgttaaaa tattattttg acctgatcca
     4921 aataaaaggc ccaccgagat ttttgaaggt cgttgtgccc ttgctgtggt cgcacaactt
     4981 ccaatccggc ttctcatttt cctccaaaaa ccaaatctcg gcttgtctga ctatcccccg
     5041 acgatatctg acctgctttt attcctcagg acaggttctg tcgagaataa aatagaccgg
     5101 gaaaaatccg gtgaatccgc tttgcagcag tgtgactcca agaattcccg gaagtaacaa
     5161 ccataaagga ttcacgtagt acccgagtgc gacgcctgct gtaataagcg cgccggcgat
     5221 tccgtcatgg agttggcgtt ttcgcttcat cttcactcct cctgtttgtt gaaacgttcg
     5281 ttcatggtct tcagggtttc cttcttatcc aagaggattt tttctggatg gttggcgatt
     5341 cttcaccttt ttagtctgtt ttactctttt tgtttcttga aatgaggacc gcatgggagg
     5401 agtctaactc cgggtacttt tttgaaaaat acgtatttaa gcctactttt tctcgtgaat
     5461 ttaaagttga ttaattgcta ggcaattcat tgaaaataca caagaattta atcatttacc
     5521 tcagtagatc tcccgtcttt tccgacaggt tcaaggggtg ctactctttg gggtgctcga
     5581 agaccttcac taagcagaat ggagaccaag acatgccttt cggctattac caccagctta
     5641 acgccaaggc gaagcggatc tatcgggcaa gcgacaaggt gtcggatttt caattgccca
     5701 atgtctctgt cgttcgaccg gtcctgcgaa aaatatttga agctttacag gccgaaagtc
     5761 atttgcgcac gcaaaaatgg actcaacgcc ttctgaatag gttaacgagc caactccata
     5821 ttcggcccat tcgatttgaa ttgttggata tccgaccctc caatccccgg atggaactgt
     5881 atggactgta ttatcccatg gaaggacgac ggatcccgcg tattcaagtg tggatgcgta
     5941 cggccaagcg gcatcaagtt gtcgcttttc gcacgtttct gcgcaccttg ttgcatgaac
     6001 tgtgtcatca tttggattac gattgtttag gcctgaaaga ctcctttcat accaaaggct
     6061 tttatggtcg tgaagccagt ttagcgtctc a
//
LOCUS       DATIAL010000309         6745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_595460, whole genome shotgun sequence.
ACCESSION   DATIAL010000309 DATIAL010000000
VERSION     DATIAL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595460"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..839)
                     /locus_tag="VLA60_09690"
     CDS             complement(54..839)
                     /locus_tag="VLA60_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09677.1"
                     /translation="MTATHVEFNAVGRTLPGVRSANRPGYAGLESQGTSTHVLLVKTG
                     IGPGKAERATRQILESEPWDVVISTGFAGALNSSPIGSLVIGQEVLLGQSTEMFSDSD
                     LPRIVCHPEWVKAALRVQLMDGCLLQGGRFVTTDRVLTQASQKRILGERTGAMAVDME
                     SGAIGEVAKQYGFPFLIIRAISDGINEDLPVDFNMFLKPFGWVGGIGQVLSSPRSWEG
                     LFRLYRNSRQAGIQLSSFFEKFFPIVSAPTFSMAINKSGAEIP"
     gene            complement(921..3089)
                     /gene="shc"
                     /locus_tag="VLA60_09695"
     CDS             complement(921..3089)
                     /gene="shc"
                     /locus_tag="VLA60_09695"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HSF09678.1"
                     /translation="MKLLKQFLVRLSGNIFETLTPKLGPYRVKQKPPPLKLVSHNLHP
                     EVSPDRTIQRTANLGHMSALESALQKGEEWLLSMQDPEKGFWVEELEADTTLTSDYVM
                     LRRFLDVIDVDRERKATRYLLHTQLEDGGWPIFFGGPADISASVKAYFALKLAGVSPD
                     EPVMQRARSLILGKGGVVQANVFTKITLALFGQYDWRGIPCMPPEIFLAPRWFYFNLY
                     AVSYWSRAVIIPLLIIFAHRPLCTISKEQGIDELFLQPRQEVDYAHVPPLHRDSTLVS
                     WRNFFVWVDGLLRLYEAYPIKALRKKALSSVQSWMIEHMGGEGGLGAIYPAMAYSIFA
                     LRALGYSTDHPLVQKALREIEALEIYSNPGNTPTPTMLHLQPCHSPVWDTALTINALI
                     ERGLALDHSSLQRADAWLRARQCRKVGDWAVSAPKARSGGWAFQFENEWYPDVDDTAA
                     VVTGLAKINSAEVLGSDEALQRGFRWALALQGSDGGWGAYDKDNNKLIFNKIPFADHQ
                     ALLDPSTADLTGRCLEMLGTLGYDQSHPAVNPAIEFLRKEQEPNGSWYGRWGVNYIYG
                     TWCVLSGLRAIGMDMTAPWIQQAVIWLESVQNSDGGWGESCTSYAEVEKAGRGESAAS
                     QTAWALMALLQAGESDSISVVRGVNWLIRHQRENGVWDEPFHTGTGFPRVFYLRYHGY
                     FKYFPIWALGMYRNVKITGEARADQLRKAAQVARRQSKLV"
     gene            complement(3271..4578)
                     /locus_tag="VLA60_09700"
     CDS             complement(3271..4578)
                     /locus_tag="VLA60_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HSF09679.1"
                     /translation="MDILEKIKNRSANVGVIGLGYVGLPLAVLQAKAGFRVFGVDEVV
                     AKVEMVNQGRNYILDVVDSELREVVEQKKLQATTDFSVLRQCDVILICVPTPLTKNKE
                     PDISAIVKVLGHLADCSHPNMLVVLESTTYPGTTEEVILPALTAKGLTAGKDLFVAFS
                     PERVDPGNAEFKTHNTFKLVGGVTKVCGEVAKAFYEQSIVKIFPLSSPRVAEMAKVFE
                     NVFRSVNIALVNELTLLCDRMNINVYEVIDAAATKNFGFMPFYPGPGVGGHCIPLDPY
                     YLAWKSKEYDVHTRFIELAGEINENMPYFVMNKLQRILNQRGKCLKDSIIFVLGVTYK
                     ANIEDPRESPATKVMELLQHEGASLQYSDPFTPSLIIQGKEYKSQQINAESLKRSACA
                     LILTAHSVFDYQLIVEHAPVVFDTRNGTRQVKHHRDRIVLLST"
     gene            complement(4687..5133)
                     /locus_tag="VLA60_09705"
     CDS             complement(4687..5133)
                     /locus_tag="VLA60_09705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSF09680.1"
                     /translation="MLTQLNDQKGFSLTELMIVVAIIGILATIAIPNFLRYQAKAKQT
                     EAKSNLVAIHTAEIAYFAENNGYIDDFNAIGFAMSGSSQRYFYKIGNANLGTLPPGCT
                     DPNLDSVSALGFTAVAIGNIDGDATCDVWTINEAKVITNVTNDVSS"
     gene            complement(5538..>6745)
                     /locus_tag="VLA60_09710"
     CDS             complement(5538..>6745)
                     /locus_tag="VLA60_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09681.1"
                     /translation="AHYYLGVIALEAKTKQEAKAHFRDAIALHPTFERAYLKLVAISE
                     EAEEPQEAIKLLERYLIEVNPHHREFRLRVIRLYIGQHNTDKALNHLDYFLQQNSDDL
                     HAQILKAQIYGEMGNFPAAIEQLNAILRVRPNELRVRDFLGLLYEEMKDYDRAIQAYR
                     TNVQMDDTFFDSILHLGFVSYRLKRNQEALSYLDQAVSLNPKRPEPYLLLGLTHFQME
                     EYHKAKARLEEGIHHDPSNAELHFNLGTVYDKLDHFDNVVREMEETLALDPEHADALN
                     YLGYSYADRGIKGEQALSLTQRAVALKPNNGYYVDSLAWALYKLGRIEEALETIQRAV
                     SLVSDDPVIYEHLGDIFLKQDERNKAREAWLHSLQLDSTNKLLGKRFREAGFGEPIPT
                     LSHQSTLSP"
BASE COUNT         1914 a         1745 c         1393 g         1693 t
ORIGIN      
        1 ccccatttct tctatcaaga aaagagtccg tttcccaata tgttgcaata atatcatgga
       61 atttccgcgc cagatttatt gatggccatg gagaaagtag gggctgaaac aatcgggaaa
      121 aatttttcaa aaaaactaga aagttggatt cctgcctgcc ttgaattccg atacaaccga
      181 aaaagtccct cccaacttcg aggtgaggat aggacctgtc ctatacctcc aacccatcca
      241 aatggcttga gaaacatatt gaagtctacc ggcaaatcct cgttaatacc atcagaaatc
      301 gcacgaataa tcagaaaagg gaaaccgtat tgcttcgcca cttcgcctat agctccactc
      361 tccatatcta cagccatcgc tccggtccgt tcacccagaa tacgtttttg tgaagcctgt
      421 gtcagcactc gatccgtcgt tacaaaccga ccaccttgaa gaaggcaccc atccattaat
      481 tggaccctta atgccgcctt gacccactca ggatgacaca caattcgtgg cagatcagaa
      541 tcagagaaca tctctgtaga ctgccccaac agaacctctt gaccgatcac cagcgatccg
      601 attggggaag aattgagagc gccggcaaaa ccagtagaaa taacgacatc ccatggttcg
      661 ctctctaaaa tttgccgggt agccctttca gcctttcctg gacctattcc cgtcttcact
      721 aacaagacat gggttgaagt gccttgactc tctagcccag cataccctgg gcggttggca
      781 gagcgcacac cgggaagagt ccggccaaca gcgttaaact ctacatgggt tgcagtcaat
      841 atgacgattc tggtcagagg aggctcctgc catcatcggg gaatgggaaa tatgcaaaag
      901 gcgggcagtc aggaccgaaa ttacacaagc ttactctggc gtctggccac ttgtgcggcc
      961 tttcgtaatt gatcggccct tgcttccccg gttattttta cattccggta catacccagg
     1021 gcccagatgg ggaaatattt aaaatatcca tgataccgta aataaaagac cctggggaac
     1081 ccggtacccg tatgaaacgg ctcatcccaa actccattct ctcgttgatg gcgaatgagc
     1141 caattgactc cccggacaac actaatggag tccgattctc cagcttgaag gagggccatg
     1201 agcgcccagg ccgtttgaga tgccgcactt tctcctcgcc cggccttctc cacttcagca
     1261 taggaagtac aggattctcc ccaaccccca tcagagttct gaacggactc cagccaaatc
     1321 acggcctgct ggatccatgg cgccgtcata tccatgccta ttgcacgaag acctgaaagg
     1381 acacaccagg tcccataaat ataattgacc ccccatcggc cataccaact tccgtttggc
     1441 tcttgctcct tgcgtaaaaa ttcaattgcg gggttcacgg cagggtgaga ctggtcatac
     1501 cctaacgttc ctaacatttc caagcatcga ccggttaaat cggccgtact gggatccaac
     1561 agagcctgat gatccgcgaa aggaatctta ttgaaaatta atttattgtt atccttatca
     1621 tatgcccccc aaccaccatc tgacccctgc aacgccaaag cccatcgaaa ccctcgttga
     1681 agggcctcat ccgaacccaa cacctcggca gaattgatct tggccaaccc cgtcaccaca
     1741 gccgcggtat catcgacatc gggataccat tcattttcaa attggaatgc ccatccacca
     1801 gaccgggctt ttggagccga cacggcccaa tctcccactt ttcgacactg acgggctcgc
     1861 aaccaggcat ctgcacgttg caatgacgaa tggtccagcg caaggccccg ttcgatcagc
     1921 gcattaatcg ttaaggctgt atcccacact ggagaatgac aaggctgtaa atgcagcata
     1981 gtgggtgttg gggtattacc aggattcgag taaatttcaa gtgcttctat ttcccttaat
     2041 gctttttgaa ccaacggatg atctgtagag tatcccaagg ctcgaagggc aaaaatcgaa
     2101 taggccattg ctggatagat ggcacccaat cctccttctc cacccatatg ttcaatcatc
     2161 caagactgta cactactcaa agcctttttc cggagggcct ttatgggata ggcctcatat
     2221 aaacgaagca gcccatcgac ccacacaaaa aaattccgcc agcttacgag agtggaatcc
     2281 ctgtgcaacg ggggcacatg agcatagtcc acctcctgac gaggctgaag aaaaagttca
     2341 tcaatccctt gttccttgga gattgtgcac agaggtcgat gggcaaaaat aattaaaagt
     2401 gggataatga ctgcacggga ccaatatgaa acggcatata aattaaaata aaaccatcgt
     2461 ggggctaaga atatttccgg aggcatacac ggtatcccgc gccaatcata ttgcccgaat
     2521 aaggctaagg taattttggt gaagacattg gcctgaacga caccaccttt gccgaggatt
     2581 aaactcctcg cacgttgcat cacaggctca tcaggagaaa ccccggccag cttgagcgca
     2641 aaataggcct ttaccgatgc actgatatct gcaggccctc cgaaaaaaat cggccaaccc
     2701 ccatcctcga gctgagtatg aagtaaatac cgagtagctt tccgctctct atccacatca
     2761 atcacatcca gaaaccgtcg aagcattaca taatcagagg tcagggtcgt atccgcttcc
     2821 aactcctcaa cccaaaatcc cttttcagga tcttgcatgc tcagtaacca ttcttcccct
     2881 ttttgaagag ctgattccag agcactcata tggccaagat tggctgttcg ttggatggta
     2941 cgatccggag aaacctccgg atgaaggtta tgggaaacca gcttaagagg cggtggcttt
     3001 tgctttacac gatagggtcc taactttgga gtcagggtct caaagatatt ccccgataac
     3061 cgtactaaga attgcttgag caacttcatg cggcagttct ttcagcgatt taacttttaa
     3121 taatgcaatt ttgggggagt gctaccaact aatgagagat tcacaaggca caaaaaagcc
     3181 ctgcttaaac agggctttat aacgaaatgc ggctagactg tcaagccgat actactttgc
     3241 cagggaatgt gttttgtatg gaaacgctcc ttaggttgaa agcaacacaa tacgatcacg
     3301 gtgatgcttc acctgacgag ttccgttccg cgtatcaaat accaccggag cgtgttcaac
     3361 aatcaactgg taatcaaaaa cagaatgcgc ggtcaaaatc agggcgcacg cactgcgctt
     3421 taacgattca gcatttatct gttgagattt atattccttt ccttgaataa taagggaagg
     3481 agtgaatggg tccgaatatt gtagtgatgc accttcatgt tgcaatagtt ccatgacttt
     3541 tgtggcaggc gattcacgtg gatcttcaat attggcttta taggttactc ccaatacaaa
     3601 aatgattgaa tcttttaagc atttccctcg ttgatttaaa atccgctgaa gcttattcat
     3661 tacgaaatag ggcatatttt cgttgatctc cccagccagt tcaatgaacc tggtatggac
     3721 atcatactct tttgacttcc acgctaaata atatggatcc aggggaatgc aatgtccacc
     3781 cacgccgggg ccaggataaa aaggcataaa gccaaagttc tttgtggctg cggcatcaat
     3841 cacttcataa acattaatat tcattcggtc acacaacaaa gtgagctcat tcactaacgc
     3901 aatattcacc gagcgaaaaa cattttcaaa gaccttcgcc atttccgcaa cacgtggaga
     3961 gctgagagga aagattttca cgatggattg ttcataaaag gctttggcaa cttccccgca
     4021 aactttggtg acaccaccca ccaacttaaa ggtgttatga gtcttaaact cagcatttcc
     4081 agggtcaacc cgctccggcg aaaatgcgac aaagagatcc tttccagccg tcaaaccctt
     4141 tgctgtcaag gcaggcaaaa tgacttcctc ggttgttcct ggataggtgg tgctttctaa
     4201 taccacgagc atattaggat gtgaacaatc agcaagatgc ccaagaacct tcacaatcgc
     4261 cgagatatcc ggttctttat ttttggtcag gggagtcggc acacagataa gtatgacatc
     4321 gcactgccgc aacacggaga aatcagttgt cgcctgtaat tttttttgct ctacgacttc
     4381 tcgtaactca gaatccacca catccaaaat ataattacgc ccttgattca ccatttcaac
     4441 cttggctacc acttcatcca ccccaaacac ccggaacccg gctttcgcct gaagaacggc
     4501 taagggcagc cccacatagc ccaatccaat cactccaaca ttggctgacc gattttttat
     4561 tttttcgagt atgtccactg atcccctcct cttgggtcac gttaaccgat tcagtttaag
     4621 atcggggaaa atcaactttt cttgagttgg ttcccgttaa atgcattgcg ctctgccaca
     4681 acaaaactag gaagacacat cattcgtcac atttgtgatg acctttgcct catttatggt
     4741 ccacacgtcg caagttgcat ctccatcaat gttacctatg gcaaccgccg taaagcctaa
     4801 tgcactcaca gaatccaaat tcgggtcggt acacccggga ggtaatgttc caagattggc
     4861 attgccgatt ttataaaaat atcgctgcga agacccactc atggcaaatc caatggcatt
     4921 aaaatcatct atatacccat tattttcagc aaaataggca atttctgcgg tatggattgc
     4981 caccaaatta cttttggcct ccgtttgctt ggctttggct tggtaacgca aaaaatttgg
     5041 aatggctatt gtcgccaaga ttccaataat tgccaccaca atcatcagct ccgttaaact
     5101 gaaacccttt tggtcgttta gttgagtcag catcctacga gacctccatg gagaactatc
     5161 ctccaacaga ttgttattta tatctcaccc tcggatgatg gtacacctgg ttaactcaag
     5221 tagccagggc tccaatgata atcaacacgg ccataaagac ggcaatgaaa cagcattaac
     5281 cgtcacaaaa ttcatataac tgaccatttc agcgacgcaa tgaatggaga gccacatcac
     5341 catgcaaggc aaataaagaa taggcgtgac tatttattcg ccagaaaaaa agcaaatctt
     5401 aagaatccgc cactgaaaat gactcagaca cgaaacctat caagtaggct cataccaccc
     5461 tttataataa actggaggta tcacttgata atttcattgg gtaaaccacg cggctaaata
     5521 tctagaatgg tgagaaccta aggggacagg gtagactggt gggaaagagt aggaattggt
     5581 tccccaaaac cggcctcacg aaatcgcttg cccagcaact tatttgttga atcgagttgc
     5641 agtgaatgaa gccacgcctc tcgtgcctta tttcgctcat cttgctttaa gaaaatatcc
     5701 cccaaatgtt cataaatcac aggatcatcg gacactaaag agaccgctcg ttgaattgtt
     5761 tccagtgctt cttcaatgcg tccaagttta tagagtgccc aagctaaact atccacgtaa
     5821 tacccattat tcggttttaa ggcgacggca cgttgcgtaa gggacaaggc ctgttccccc
     5881 ttgatgccac gatcggcata gctatacccc aaataattca aggcatccgc atgctcagga
     5941 tccaacgcca gagtctcttc catttccctg accacattat cgaaatggtc taatttgtca
     6001 tagaccgtgc ctaaattaaa atggagttcg gcatttgagg ggtcatggtg aataccctcc
     6061 tctaatctgg cctttgcctt gtgatactct tccatttgaa aatgggtcag ccccaacaat
     6121 aaatacggct ctggccgctt gggattgagg ctaacggcct gatccaaata cgaaagcgct
     6181 tcctgattgc gttttaaccg ataagacaca aagcctaaat gcagaatact atcgaaaaaa
     6241 gtgtcgtcca tttggacatt tgtccgatag gcttgaatcg cgcggtcata atccttcatt
     6301 tcctcatata acaatcccag gaagtcacga acacgcaatt cgttaggtct cacgcgcaag
     6361 atggcattca attgctcaat ggcagctgga aaattcccca tttcaccata gatttgagcc
     6421 ttaagaatct gcgcatgcaa atcatctgaa ttttgctgga gaaaatagtc caaatggttc
     6481 aaagccttgt ccgtgttatg ttgtccaata tacagccgaa ttacacgcaa ccggaattcc
     6541 cgatgatgcg gattaacctc aatcaaatag cgctccaaaa gtttaatagc ctcctgaggc
     6601 tcctctgctt cttcggaaat ggccactaac ttcaggtatg ccctttcaaa agtagggtga
     6661 agcgcaatgg cgtcccgaaa atgtgccttg gcttcctgtt tcgttttcgc ctccagagca
     6721 atcaccccca aataataatg ggcct
//
LOCUS       DATIAL010000310         4282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_165354, whole genome shotgun sequence.
ACCESSION   DATIAL010000310 DATIAL010000000
VERSION     DATIAL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165354"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VLA60_09715"
     CDS             complement(<1..1136)
                     /locus_tag="VLA60_09715"
                     /inference="COORDINATES: protein motif:HMM:NF027515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HSF09682.1"
                     /translation="MAYEHLLALRGELVRQQLTGFVVPHTDEYQNEYLPPCAERLAWL
                     TGFTGSAGTAIVLRDEAAMFVDGRYTLQVAYQVDKKSFTLHNSGETSPEEWLALRAVP
                     TDRIGYDPWLHTPRDLERFQTATERVGAQLVPCVSNPIDGVWHDRPQPPSGVIKPHLL
                     EFSGQSSQIKREILSRRFVEDRIDAAIITAPDSIAWLLNIRGSDVAHTPLPLCGAILY
                     ATGRVALFVDPKKITPGLQAHLGPDISFALPEELEAGLQQLGTHGNRVLCDPAKTNSW
                     IFTTLTQSGAALVHKDDPCVLPKACKNPVEIAGAYSAHQRDGVAMCQFLAWLAREGPK
                     GQVTELEAVDYLDACRRKQVFWEDCSFPTISGAGSNGAIVHYHST"
     gene            complement(1310..1645)
                     /locus_tag="VLA60_09720"
     CDS             complement(1310..1645)
                     /locus_tag="VLA60_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09683.1"
                     /translation="MQVAERMQDTTLVLMISGRVTAYSRKVFQGMVKSARFSGARHII
                     FNMEGVTFMDTIGLGGLVLAYLDLNDNHMAMSVVEPQQPVKTLLEDANFSELVPTYPT
                     EETALQAIH"
     gene            2013..4238
                     /gene="recQ"
                     /locus_tag="VLA60_09725"
     CDS             2013..4238
                     /gene="recQ"
                     /locus_tag="VLA60_09725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HSF09684.1"
                     /translation="MKNNLSPQHTDSRHLHSSTQPHLLSLLKEHFGFTAFRPLQEEII
                     RDVLAERDVVALLPTGGGKSLCFQLPALARSGLTIVVSPLIALMKDQVDGLRANGIPA
                     TYLNSSLTAQESQIRLRGLHQGDYRLLYMAPERLMVPGYLSLVRNFQVNLLAIDEAHC
                     ISEWGHDFRPEYRRLVELRDQVPSLPIIALTAAATERVQKDIVTLLRLQNPACYVASF
                     NRPNLTYQVQPKDNPFGQVIAFLRKRPKDSGILYCQSRKTTESVADRLQHHGLKALPY
                     HAGLSAEERTRHQELFLRDEIQVICATIAFGMGINKPNVRFVIHYDLPKNLESYYQET
                     GRAGRDGLPSECVLLFSAGDTVKQQRFINEKSNFQEQQIANRQLQEMVHYAECATCRR
                     RTLLQYFGEQVESENCGGCDNCLTPRDIFDGTDAAQTVLSCVEEIRGTSGFSVGLNHV
                     IAVLTGANTEKIRRWRHDQLTSYGKGEAHRRPEWAAISRELIRLGYLQQTADPFTVVE
                     VTDQGQHFLQDPKTLMLTTPMSTPERKALSGEDLSHDEMLFTRLRQLRKTLADEADVP
                     AYVIFSDVALRQMARDYPTNEQEFLQISGVGNRKLQEFGPKFLAVIARHLENYAPLTI
                     PKESNPLPAPSVSDRRKSLGDTVHETLRRFQSGHSVEQIASERGLVTSTIYGHLEQAI
                     KAGEPVDLTQLWTTEQDLKMAAAFEKTGFGNLTGAKELLGNLYDYSQLRLFRAVYGKK
                     G"
BASE COUNT         1064 a         1214 c         1066 g          938 t
ORIGIN      
        1 gtcgaatgat aatgcacaat ggcgccattt gaccctgcac cagaaattgt agggaagctg
       61 caatcctccc agaacacctg ttttcggcga caggcatcga gatagtccac ggcctctaat
      121 tctgtcacct gtcccttggg accttcccgg gccagccacg ccagaaattg acacatggcc
      181 actccatccc gttgatgcgc cgaataggct cctgcaattt ccaccggatt tttacaggcc
      241 tttggcagca cacagggatc atctttatgt acaagggcag ccccactctg ggttaatgtg
      301 gtgaaaatcc aggaattggt cttggctgga tcacacaaca cccgattccc atgggtcccc
      361 aactgttgta agccagcttc aagttcctcc ggtagggcaa aggaaatgtc cggacccaaa
      421 tgtgcctgca agcccggggt gatttttttc ggatcgacaa acagggcgac ccgcccggta
      481 gcgtatagaa tggccccgca caatggtagt ggagtatggg ctacatccga tccgcgaatg
      541 ttcaacaacc aggcaatgga atcgggtgcc gtaatgatgg ccgcatcgat ccggtcttcc
      601 acaaacctcc ggcttaatat ctcacgcttg atctgggaac tttgacccga aaactccagg
      661 agatgaggct tgatcacacc tgagggtggt tggggccgat catgccagac gccatcgata
      721 ggattcgata cacaggggac gagttgtgca ccgactcgtt cagttgctgt ctgaaaccgt
      781 tcgagatccc gcggggtatg aagccagggg tcatagccga ttcgatcggt tggtactgca
      841 cgaagtgcca accattcttc cggtgaagtt tctccactat tgtgaagcgt aaagcttttc
      901 ttatcaacct ggtaagccac ctgaagcgta taccggccat ccacaaacat tgcggcttca
      961 tcccgtaaga caatcgccgt ccccgccgaa ccggtaaacc cggtcaacca ggcaagccgc
     1021 tctgcgcagg gaggcagata ttcattctga tattcatccg tatgcggcac cacaaagccc
     1081 gtcaattgtt gacggaccag ctcccctcgc agcgccagaa gatgctcata tgccatcaca
     1141 actttcctat tattctaaac attccggttt cactccttcc cctgagcgag cccttgcgag
     1201 gataacacat tcgcgactgt ctgccgattg tttaaacaca cgtaccccgc gtagggcaaa
     1261 tcgactcaca tcattttaaa cgatgaaaaa gggaagcgca cccggaaaac taatggatgg
     1321 cttgcaaggc agtctcttcg gtgggatacg tcggaaccag ttcggaaaaa tttgcgtctt
     1381 ccagtaaggt tttcaccggc tgttggggtt ctacgacgct catggccata tgattgtcat
     1441 tcaagtcaag gtaggcgagt accagccccc ccaacccaat ggtgtccatg aacgtaacac
     1501 cctccatatt aaaaatgatg tgcctggcgc cggagaacct ggcggatttc accatcccct
     1561 ggaacacttt ccgggagtac gcggtcactc gtcctgaaat catcaagacc agcgttgtat
     1621 cttgcattcg ttcggctact tgcatctcgg caccaggggt tattttgtta ttttcggcaa
     1681 tttcctccga ttcttgctgg atgcccctga tttcctcatg atgtcgaggg tcttttacaa
     1741 cacacaggta gacagcctca ttatggaaag agcattatga cggatcttgg gccgtccacc
     1801 ggtaaaaaat aacataccga aattacgaag aaactgagag aaaatacagt atatcccgcc
     1861 tgagcggcag aaagctgtcg gagtgggcaa tgagaaggca accgagggtc actgggggaa
     1921 tcttcccccc acaagatgag cctcgctgca gatcagaata tctctatcaa tactctccat
     1981 caacatctct tacatttgcc acaacttcaa ttgtgaagaa caacctatcc cctcaacata
     2041 ccgactcccg gcacctgcac tcctcaacac aacctcacct cctctctctg ttaaaagaac
     2101 attttggttt cacggccttc cggcctttac aagaagaaat cattcgggat gtcctcgctg
     2161 agcgcgacgt ggtggccctg ttacccacgg gaggagggaa gtctctgtgc tttcagttac
     2221 cggcgctggc acgctcgggc ctgacgatcg tggtctcgcc gctcattgcg ctgatgaaag
     2281 accaagtgga tgggctccgg gccaatggca ttcccgctac gtatctcaac tcatctctga
     2341 ccgcgcagga atcccagatc cgcttgcggg ggctgcacca gggagactac cgcctcctct
     2401 acatggcacc ggaacgcctc atggtcccag gatacctcag cctggtcagg aattttcagg
     2461 tcaatctgct ggccattgat gaagcccact gtatcagtga atggggccat gatttccggc
     2521 cggaataccg gcgacttgtg gaactccggg atcaggtccc gagtctcccg atcatcgcgc
     2581 tcaccgccgc cgccacggag cgggtgcaaa aagacatcgt cacacttctg cgattgcaga
     2641 atccggcctg ctatgtggcc agtttcaacc gtcccaacct gacctaccag gtgcagccaa
     2701 aggacaatcc gtttggacaa gtcatcgcat ttctccggaa acgccccaag gactctggaa
     2761 ttctctactg ccagagccgg aagaccacgg aaagtgtggc ggatcgttta cagcatcatg
     2821 gcctgaaggc actgccctat catgccggcc tgagtgccga ggagcgcaca cggcatcagg
     2881 aattgttcct acgagatgaa atccaggtga tttgcgccac cattgccttc ggcatgggaa
     2941 tcaacaagcc taacgtgcgc tttgtcattc actacgattt accaaaaaac ctcgaaagct
     3001 attatcaaga gaccgggcgc gccgggcggg atggcctgcc aagcgagtgt gtcctgctct
     3061 tcagcgcagg ggatacggtc aagcagcaac gcttcatcaa tgagaaatct aacttccagg
     3121 agcagcagat cgccaacagg caattacagg aaatggtgca ctatgcggaa tgtgccacct
     3181 gccggcgccg cacattgctc caatattttg gagaacaggt tgaatcggaa aactgtggcg
     3241 gatgtgataa ctgcctgacg ccgagggata tcttcgacgg cacggatgcc gcgcagaccg
     3301 ttctctcttg cgttgaagag atacgtggaa caagtggatt tagcgtcgga ctcaatcacg
     3361 tcatcgccgt tctcaccgga gccaacaccg aaaaaatccg acgatggcgt catgaccaat
     3421 taaccagcta cgggaaaggg gaagcccacc ggaggcctga atgggctgcc atcagccggg
     3481 aactcatccg gctgggatat ctccaacaaa cggcagaccc attcacagtg gtggaagtca
     3541 ccgaccaggg tcagcatttt cttcaagacc ccaagacgct catgctcaca acacccatga
     3601 gcacaccgga acgtaaggcg ctctctggcg aggatcttag ccacgatgag atgctcttca
     3661 ctcgcctccg tcaattgcga aagacattag ccgatgaggc tgatgtccct gcctacgtca
     3721 tattctccga tgtcgcacta cggcagatgg cacgagacta tccgaccaat gaacaggaat
     3781 tcctccagat tagtggcgtc gggaatcgga aattacagga atttgggcct aagttcctgg
     3841 cagtcattgc caggcacctg gaaaactacg ctcccctaac tattccgaaa gagtcgaatc
     3901 ctcttccagc accatctgtc tctgacagaa gaaagtcgct tggagatacc gtccatgaaa
     3961 ccttgcggcg ttttcagtcg gggcattctg tcgaacagat cgccagtgag cgcggcttgg
     4021 tcaccagcac catctatgga cacctggaac aagccatcaa agccggtgag ccagtagacc
     4081 ttacccagct gtggacaacg gagcaggatt taaaaatggc agcggccttc gagaaaacgg
     4141 ggtttggcaa tctcaccggc gccaaagagc tactaggcaa cctctacgat tacagccaac
     4201 tccgcctctt ccgcgccgtt tatggcaaaa agggttaaga attcaatcag atctcatttc
     4261 aggcattagt tattccaggt gg
//
LOCUS       DATIAL010000311         3553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_810487, whole genome shotgun sequence.
ACCESSION   DATIAL010000311 DATIAL010000000
VERSION     DATIAL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810487"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VLA60_09730"
     CDS             complement(<1..690)
                     /locus_tag="VLA60_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09685.1"
                     /translation="MMTPGQQHRLVLSNQVGGTVVADAIKFQAAGSGTPRRATWGLSV
                     PTTGTYNVYARWTADAANATNASYTVYYAGGTTAVPMNQTQNGGQWNLLGTFTLNPAS
                     NPMVELSDQANGLVVADAVMVVPSGVSTDHVTYTPTLTTAGKVDIYAKWTENATRAGS
                     DLHRSACGGRDRYSGESTAVQCGVVSVGSLCDVARTESSCGSGRSLGGRDGGGCDTVC
                     EWGHECARDQLC"
     gene            839..970
                     /locus_tag="VLA60_09735"
     CDS             839..970
                     /locus_tag="VLA60_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09686.1"
                     /translation="MDRDMYVRGIIGPWFGDWISEQAVHPQPGRNLLLKLPMLLLYK"
     gene            998..1088
                     /locus_tag="VLA60_09740"
     tRNA            998..1088
                     /locus_tag="VLA60_09740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1032..1034,aa:Ser,seq:gct)
     gene            1221..2282
                     /locus_tag="VLA60_09745"
     CDS             1221..2282
                     /locus_tag="VLA60_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09687.1"
                     /translation="MREHRWETQATLSFEDILSVAGKLRQLGLTSIHEDKEMIGYIEE
                     WEVDHPQRIQALALWPTEDVTLLHLLDNWQGDFFLLAGRYHSIFQTHQSVNTYCSIAH
                     PWRITQPLTTLLPEAWLWLGFRHTHGFIRVRVHTTEVITPGETLAKPRDRFWLTDREN
                     AFRTAIQILDLPIEVTQKGARMLLQTDRTDTPLFCSWPDAFGPCQFELNSPDPFEFLV
                     PASQLAATYQGKSAHVRVYLTGFPEPTLLDFTGIAPNPRFMYRCSIHCTLSDMPELLH
                     LLEPQGRVYGSLAEFQTDYLLAEGEDVAAIVGLVGTNGDFRLEIRLNQRPLPHQATEQ
                     WLEELVGHPLVYAPLPAFP"
     gene            complement(2454..3452)
                     /gene="rfaQ"
                     /locus_tag="VLA60_09750"
     CDS             complement(2454..3452)
                     /gene="rfaQ"
                     /locus_tag="VLA60_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HSF09688.1"
                     /translation="MKRLLLIKLRYIGDVVLSTPLLPILHKRFPNASLTFLVNPGTES
                     VLFGNPYLENIMVLPREGWRQKFKCYQSLRQARFDGVVDLTDGDRSAFLTYWTGAGVR
                     LGFNRENRWRGKLYTHVLPSAYGSMHMVDYHAQALPALGINDPVGNPEWYLQPEDHEQ
                     GDHLMVSLQIGQAPLVLLHPPARYANKAWPLERFAALADWLADHGAVVALIGHESEML
                     IGHRIVNLTRSKPRNVMGQTGLRQLAAVMKRSALFIGNDGGPMHMAAAVGCPVLGLFG
                     PSDPYVWGPRGEKVTVFYKGLDCEKCCHDGCSRGEEGCMRQISVEEVCRAARLFLG"
BASE COUNT          808 a         1066 c          879 g          800 t
ORIGIN      
        1 acataattga tccctggcgc actcgtgccc ccactcacaa accgtatcgc atccgccacc
       61 gtcacgccct ccaaggctcc tgccacttcc acatgatgat tctgtcctgg cgacatcaca
      121 aaggctccca accgaaacca ccccgcactg gactgctgtc gattcaccag aatatcggtc
      181 gcgcccccca catgctgaac ggtgtaggtc actgcctgcg cgggtggcat tttccgtcca
      241 tttcgcatag atgtccacct ttcctgctgt ggtcaacgtc ggtgtatacg tcacatgatc
      301 ggtgctgacg ccgctgggca cgaccatcac cgcatcggcc accaccaggc cattggcctg
      361 atcagacaat tccaccatcg ggtttgacgc gggattcaag gtaaaagtgc cgaggagatt
      421 ccactgcccg ccgttttgcg tttgattcat tggcaccgcc gtggtgccgc ccgcatagta
      481 cacggtatag gaggcgttgg tggcattggc cgcatcggcc gtccaccggg catagacgtt
      541 ataggtaccg gtggtcggca ccgacagacc ccacgtcgcc ctgcgcggcg tccccgaccc
      601 cgcggcttga aacttgatgg catccgccac caccgtgccc cccacttgat tggataagac
      661 caagcgatgc tgctgtcccg gcgtcatcac ataggtcccc aacacattcc attgaccgaa
      721 ggtttatttg ggaaaatccg cgacccgatg gcctatctct gcgcctccta tcatccgaca
      781 aaaagcttgt gaaaaatata tccaagaacc gtttgggcat ggtaggatca ccaccctgat
      841 ggatagggac atgtatgtga gggggatcat tggcccatgg tttggggatt ggatttccga
      901 acaagcggtt catccgcaac ccggccgcaa tctgctcttg aagttaccca tgctgttact
      961 ctataagtag tcacaatcaa ttgacataca cttgcgcgga gaggtggccg agtggccgaa
     1021 ggcaacggtt tgctaaaccg tcgtaggtca aaagcctacc gcgggttcaa atcccgccct
     1081 ctccgccata tttttcgtca aattttccgg atctgccttc gttccttaac cttcatccca
     1141 ttcccctggt cacctccacc cggtatcgtt ggaaccaaat gatccacctg ccccgcattt
     1201 tgctttgctg aataagaccc atgcgtgaac atcgttggga aacccaagcg acgctctcct
     1261 tcgaggacat tttatcggtg gcgggcaaat tgaggcagtt aggcctaacc tccatccatg
     1321 aggacaagga gatgatcggc tatatcgaag aatgggaggt ggaccatccc cagcgcatcc
     1381 aggccctcgc gctctggccc accgaggatg tcacgctatt acatctgctc gacaactggc
     1441 agggcgactt ttttttattg gcaggccgct atcactccat ctttcaaacg catcaatcgg
     1501 tgaacacata ctgttcgatc gcgcatccct ggcgtataac ccagccgttg acgacactcc
     1561 tgccggaggc ctggctgtgg ctgggatttc gacataccca tggattcatt cgggtccggg
     1621 ttcataccac cgaagtgatc acacctgggg aaacacttgc caaaccacgg gaccgatttt
     1681 ggctcacgga ccgggaaaac gcctttcgga cggcaattca gatcctggat cttccgattg
     1741 aggtgacgca aaaaggcgca cgaatgctgc tgcagacaga tcgaaccgat accccgctct
     1801 tttgctcatg gccggatgcg tttggcccct gccaattcga actcaactcg ccggatccct
     1861 ttgagttctt ggttccggcc agccaactgg ctgcgaccta ccagggcaaa tctgcgcatg
     1921 tgcgtgtgta tctcacggga tttcccgaac ccaccctctt ggatttcact ggcatcgcac
     1981 ccaatccccg gttcatgtat cgctgtagta ttcactgcac cctgtccgat atgcctgagt
     2041 tgcttcatct cctggagccg caagggcggg tgtatggcag cctggcagaa ttccagaccg
     2101 actatctcct tgctgaaggt gaggacgtgg cggccatcgt gggtctggtg gggacgaatg
     2161 gtgactttcg tctggagatt cgcctcaacc aacgacctct tcctcatcag gccacggaac
     2221 aatggctgga ggaactggtt ggccatccac tcgtctatgc acccctaccc gccttcccgt
     2281 aaagggaatg ttaaaaatcc tgccagaggc gtttatggag acgccttttt aagcgctccc
     2341 tcattgtcct caggttggtt tctgtggctc atatgcccat ggaaaaagca ccatatttga
     2401 aatattcaac aactcccgtc acaaaatcct ctaatgcctg agtccacgaa cactcaaccc
     2461 aggaacaggc gtgcggcccg gcagacttcc tcgactgata tttgccgcat acacccttct
     2521 tctccccggg aacacccgtc atgacaacat ttttcgcagt ccaggccttt gtaaaacacg
     2581 gtaacttttt ctcccctcgg accccacaca taaggatcag agggaccgaa taatcccaga
     2641 acaggacaac cgacggctgc cgccatatgc atcgggccac catcgttccc gataaataag
     2701 gcgcttcgct tcatcactgc cgccagttga cgcaacccgg tttgtcccat cacattacgt
     2761 ggtttggacc tggtcagatt gacaatccgg tgtcctatca gcatctctga ctcgtgcccg
     2821 atcaaggcaa cgaccgctcc atgatcggcc agccaatcgg ccagggcggc aaatcgctca
     2881 agaggccagg ctttattggc atatcgcgct ggagggtgaa ggagcaccaa tggcgcctgc
     2941 ccgatctgga gcgagaccat caggtgatcg ccttgctcat ggtcttcggg ttgcagatac
     3001 cattccggat tgcccacggg atcattgata cctaaggcgg gcagggcctg ggcatgatag
     3061 tccaccatgt gcatagaacc ataggctgat ggcagcacat gcgtatacag ctttccacgc
     3121 caccgattct cccgattaaa ccccagcctg acaccggcac cggtccaata ggtcagaaag
     3181 gcggagcgat ccccgtccgt taaatccacg actccatcga aacgagcctg acgcagcgat
     3241 tggtaacact taaatttttg tctccatcct tcacgaggca acaccatgat gttctccaga
     3301 tagggattcc cgaacaacac actctctgtt ccgggattca ccagaaaggt gagtgacgca
     3361 ttgggaaatc gcttgtgcag aatggggaga agaggggtgg ataacacgac atccccaata
     3421 taccgaagct taataagaag aagacgtttc acgtgaatcg tgacttcgag atgatcctga
     3481 aaaaataatc ctacagggga accgttcggg ttacactcgg agggggcttc atttcaggaa
     3541 tccgtttgat gga
//
LOCUS       DATIAL010000312         7148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1132906, whole genome shotgun sequence.
ACCESSION   DATIAL010000312 DATIAL010000000
VERSION     DATIAL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132906"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VLA60_09755"
     CDS             <1..495
                     /locus_tag="VLA60_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSF09689.1"
                     /translation="EDENKKEVKYWRDIGTLDAYYEANMDLVGVDPHLNLYDEAWPIR
                     TYQGQYPPAKFVFADEFKGGRRGMALDSIVSGGCIISGSLVQNCVLSPNVHIEHHAEV
                     HDSVIMENVKIGDRSRIRRAIIDKGIVIPPDTIIGHDLHHDRQRFPVTEDGVVVVTPD
                     SPTK"
     gene            complement(536..1306)
                     /locus_tag="VLA60_09760"
     CDS             complement(536..1306)
                     /locus_tag="VLA60_09760"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HSF09690.1"
                     /translation="MLSSRDRITILSALIGVIILAWAYVLFLWAKMPMMHADVPEAIM
                     QLHPWTPVDFVFGYLMWAVMMVGMMLPGAVPMTLLYAGMVRKAERQGSPMAPTGAFVA
                     GYLALWSVFSVGATVVQWGLHATAMLSPMMVAKSPVVGAGLLVVAGMYQLTPWKAACL
                     EHCRAPAHFIAEHWRPGTSGAFRLGFHHGAFCLGCCWALMGLLFVGGVMNVLWIAAIT
                     VFVFLEKILPVGPWSGGLGGVAGIGLIVSGVGVLVLAS"
     gene            complement(1612..2247)
                     /locus_tag="VLA60_09765"
     CDS             complement(1612..2247)
                     /locus_tag="VLA60_09765"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HSF09691.1"
                     /translation="MADQWMLRGVEFSNCNCTYGCGCQFMAPSTNGFCEAMGSGHIEE
                     GYFNDTRLDGLNYVMLLQWPGEIAQGNGTQQILIDARADALQREALRKILHGEATAPG
                     STHFFVFNSTMSNVLEPQFVPIDLSIDVEARQATVKVPGLVESKGTPISNPHTGRDHR
                     ARIHLPEGFEYTVAEVGNASTKAQAGIRLNLSNSYGQFNILHMNQDGVIRP"
     gene            complement(2756..3082)
                     /locus_tag="VLA60_09770"
     CDS             complement(2756..3082)
                     /locus_tag="VLA60_09770"
                     /inference="COORDINATES: protein motif:HMM:NF002586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HSF09692.1"
                     /translation="MTTMYWYAGAALAEISGCFAFWAWLRLGRSGWWTVPGMVSLIVF
                     ALLLARTEAAFAGRAYAAYGGVYIAASLLWLWGVEQIQPDRWDWLGAGLCLVGAAVIL
                     WGPRPA"
     gene            complement(3115..4344)
                     /locus_tag="VLA60_09775"
     CDS             complement(3115..4344)
                     /locus_tag="VLA60_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF09693.1"
                     /translation="MVDPVSSPNSSHSDRPRIPTSIWALGLVSLCMDMSSELIHSLLP
                     VYMVTVLGASLLSVGLIEGFAEATALLTKMFSGVLSDYWGKRKLLTVIGYGMAAVTKP
                     LFPLASSLTTVFLARFLDRVGKGIRGAPRDALIGELAPPNLRGTCYGLRQSLDTVGAF
                     LGPLMAMGLMVLLADDIRAVLWVAVIPAVLAVFLLIAGVQEPPSTRPVGGSQTGIRLS
                     DLQRLVPAYWWIVVSGTLLTLARFSEAFLVLRAEHLGLSVTLIPMVMVVMSVTYAASA
                     YPAGRLADKMSRRPLLVAGCLFLMVADAILAVAGDVVMVMIGVAFWGMHMGLTQGLLA
                     TLIADASQPELRGSAFGVFSFVCGMSLLCASVLAGFLWDQFGPSATFLCGAVLTAVAL
                     LGFVLHNPDTPHPKRRE"
     gene            complement(4420..5763)
                     /locus_tag="VLA60_09780"
     CDS             complement(4420..5763)
                     /locus_tag="VLA60_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSF09694.1"
                     /translation="MTPIISTLGGPDVTHIFVHDPVALAKNLPLERRPANFRAYDQRA
                     LVMARPHDVVCMTSSKVDLSYMGFLYELGVGPEPGNIIELEPVPVSGECLDNDLFCPS
                     VDSLHRICERIPPHNHVVLNPDAGSQKGYVMAQALENLLGRTVKCMGGSPEVTEAANL
                     KHVAFRKAQELGIPVARGEIVESSDGQAPNVTRLRSAIRRYVAETGRVIIRSAEGRGG
                     TGIFLVEDNPESERQALELISRRPLSPVYLVQVLYEVAFSPNILVDVQPDGGAVNVVG
                     ISDQRLNPHLGHIGNVSPSGAHMLNDMSRAAEALGRWLQEDGYTGLVGFDFVEHADLR
                     TGRRRWFLAEINARSNASVYPTFLMAHLNVRQMDRGFPVIGAFFSMLARIQTVSFREF
                     RNAFDARLFNPRTGRGMIPYGFCQEQLCCNMVFLGPSRHEVEAMHTACVAQLSGR"
     gene            complement(5760..6965)
                     /locus_tag="VLA60_09785"
     CDS             complement(5760..6965)
                     /locus_tag="VLA60_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09695.1"
                     /translation="MVRKGAGNIALVLIGCGIAFVLVEIGLYLFGISYPSFFIADNLT
                     GYAHKPGAEGLYSNEGEAYIRINSLGLRDREHPLEKPVGTFRIALLGDSYTEAMQVPT
                     EQIYGARLEHELSDCPSLAGRQVEAMNFGVSGFSTAQELLVLRHKASPYDPDLVLLAF
                     YSENDIRGNLRELNGKTNIPYFIMNDGRLVLDNSFHHTTEFWLQQFPLSAEAFEISRV
                     LQVFRNAKYTLKSFLEEMAQQQQMKDWEDPALRLDLLVYLDQPDPSWENAWTITERLI
                     VQMKREVDDGGRRFLLVSLSNPDQVHPDVQHRQALARQLGVPDLLYPDRRIQALAKRE
                     GIELLSLAEPLATYAEQHGVFLHGFQNTALGKGHWNAAGHRLAGKLMSERICRIMESI
                     VPGKAIMAE"
BASE COUNT         1912 a         2161 c         1713 g         1362 t
ORIGIN      
        1 gaagatgaaa acaaaaaaga ggtcaagtac tggcgggaca tcggtaccct ggacgcctac
       61 tacgaagcca acatggatct cgtcggggtc gatccccacc ttaacctcta tgatgaggcc
      121 tggcccatcc gcacctacca aggccaatac ccgccggcca aatttgtctt tgccgatgag
      181 ttcaagggag ggcgcagagg tatggcactc gattccatcg tctctggagg ctgcattatc
      241 tccggaagcc tggtgcaaaa ttgtgtgctc tccccaaacg tccacatcga gcatcatgcc
      301 gaagtccacg attccgtcat catggaaaat gtcaaaatcg gcgaccggtc ccgtatccgc
      361 cgggccatca tcgataaggg catcgtcatt cctcccgata ccatcatcgg gcacgatctc
      421 caccacgatc gccaacgctt tcccgtcacc gaagacggcg tggtcgtcgt tacacccgat
      481 tcacccacaa agtaatcgag ccgtcgtgga aggggcacac cccaaaacgg gcaattcatg
      541 acgccagaac caacaccccc accccactca caatcagacc gatcccggcc actcctccca
      601 accctccgct ccaggggccg accggcaaga tcttttctaa aaacacaaag accgtaatcg
      661 ccgcaatcca gagcacattc atcaccccac ccacaaacaa cagccccatc aaggcccaac
      721 agcacccgag acaaaatgca ccatgatgaa atcccaatcg aaacgcgccg gaagtgcccg
      781 gtcgccaatg ctccgcaata aaatgagcgg gtgcacgaca atgttctaaa cacgcagcct
      841 tccacggggt gagttgatac atgcccgcga caaccaagag ccctgcaccg acaacaggac
      901 ttttggccac catcatgggc gagagcatgg ccgtggcatg caatccccac tgcacgacag
      961 tcgcgcctac actaaagaca ctccatagag ccaaataccc ggccacaaac gcccccgtcg
     1021 gagccatggg tgaaccctgc cgctcggcct tgcgaaccat acctgcatac aacaaggtca
     1081 tcggaacggc acccggcagc atcatcccga ccatcatgac agcccacatc aaatacccaa
     1141 acacaaagtc caccggcgtc catggatgca actgcatgat cgcttcgggc acatcagcat
     1201 gcatcatggg catctttgcc cagagaaaca ggacataggc ccaagccaga ataattaccc
     1261 caatcagggc tgagagaatc gtgattcgat cacgcgagga taacatagga aatgaaggcg
     1321 caggagaggg actcatggaa atggactata agaacccctg aaacaatcag caactgacag
     1381 gtaggttgaa taaccagaag cggacacctt ggcctgagag aacgttccct tcctcaccag
     1441 aagggcgaag tccgcgtagg cgcgactgtc aggattcccg cttaggggct gtcctgagcc
     1501 aaaccgcgag acccgcaacg gcgtcgtcaa cacgctgcac attcaacatg gcagaaaatg
     1561 atgatggccg acgaagaagt cgttcacaag gatagacgca tcgctcaaca atcagggtct
     1621 gatcacgcca tcctgattca tgtgaagaat attaaattgc ccatagctat tagacaggtt
     1681 caacctaatt ccggcttgag ccttggtcga cgcattgccc acttcggcca cggtatattc
     1741 aaatccttcc ggcagatgaa ttctggcgcg atggtctctt ccggtatgag gattggagat
     1801 gggtgtaccc ttcgattcca ccaagcccgg caccttgacc gtggcctgtc ttgcctccac
     1861 atctatggag agatcaatcg gaacaaactg cggttccaac acgttggaca ttgtactatt
     1921 gaacacgaaa aagtgggtag accccggggc ggtcgcctcg ccatgtagta ttttccggag
     1981 ggcttcgcgt tgcaaggcat ctgcccgcgc atcgattaaa atctgctgtg ttccatttcc
     2041 ctgagcaatt tcccccggcc attgcaacag catgacatag ttcaatccat ccaaacgcgt
     2101 gtcattgaaa tatccctctt caatatgccc cgagcccatg gcctcgcaaa atccgttggt
     2161 cgagggggcc ataaactggc acccacaccc ataggtacag ttgcaattgg aaaactcgac
     2221 accacgaagc atccattgat cagccattgt gcgctccttt cgaaagtcat atcggacccc
     2281 tctcaattat ttccagtttt tatcatgccg ctcaccgacg tttccaccgc caccccgggc
     2341 attggcccat ccaccagagg tcttcgtcaa gaacctctct caccgcgtga gagtcatcta
     2401 cacgttccga cttttcccat cacgccacct atactttttc tccctggttc gctaccattc
     2461 gcctccgaac ggcaccatag aaaagccgca ccactcacaa cacaaggagc acaccatggg
     2521 caagagccag gacgcacgca aagaaaccaa aaagaagcca gcgaaaacca tgaaagaaaa
     2581 gcgggccgag aaaaaaggca aaaaatagct tagggcattc gtgcccaatc ggcgaaaacc
     2641 ggcgagttga cgaggcggaa ggaaacaccc cctcgtcaac gattagaaga ttcccctcag
     2701 catctgccaa cctgactatg accacccacc gcgaaatgcg ttgctctcca atgtattaag
     2761 ccggacgagg gccccaaaga atcaccgccg ctccgacaag acacagcccc gccccgagcc
     2821 aatcccaacg atccggttgg atctgttcaa caccccagag ccataagaga gaggccgcga
     2881 tatatactcc gccgtatgcg gcataggctc gacccgcaaa tgcagcttcc gttcgcgcca
     2941 ataacagggc gaacacaatc agactcacca tgccgggcac ggtccaccac cccgaacgcc
     3001 caagtcgtaa ccaggcccaa aaggcaaaac acccgctaat ttccgccagt gctgccccgg
     3061 cataccaata catggtagtc atatcttctc cttattccgt gaaaccacaa ggcatcattc
     3121 ccgtcgcttc ggatgaggcg tatccgggtt atggagaaca aacccgagca gtgcgacagc
     3181 cgtcaacacc gctccacaca gaaaggtggc ggagggaccg aactggtccc agaggaaacc
     3241 agccagtaca ctcgcacaga ggagtgacat gccgcagacg aaactgaata caccgaaagc
     3301 gctcccgcga agctcaggct gtgaggcatc cgcaatgagg gtggccagga ggccttgcgt
     3361 gaggcccata tgcatacccc agaacgcgac cccaatcatc accataacca catcaccggc
     3421 aacggcaaga atcgcatcag ccaccattaa gaacagacat cctgccacca acaacggacg
     3481 gcgactcatc ttatccgcca gcctgccggc cggataagcc gacgcggcat aggtcacgct
     3541 catcacgacc atcaccattg ggatcaatgt caccgataac ccgagatgtt cggcccgtaa
     3601 aacaaggaac gcttcactaa atctcgccag ggttaacagt gttccagaca caacaatcca
     3661 ccagtaggcc ggaacaagcc gctgcagatc actcagccga atacccgttt gagaccctcc
     3721 cacaggacga gtggagggag gctcctgaac gccggcaatc agaagaaaca cagccagcac
     3781 cgcggggatg acggcaaccc acaacacggc ccggatatca tcagccaaca gaaccatcaa
     3841 ccccatggcc atcaacggcc ccagaaacgc gccgacggta tcgagcgatt ggcgaagccc
     3901 gtaacacgtc ccgcgcaaat tcggcggggc caactctcca atcagcgcat cacgcggggc
     3961 cccacgtatg cccttgccga cgcggtccag aaaccgcgcg agaaacacgg tagtcaggct
     4021 ggaggctaag ggaaataagg gtttcgtgac cgcagccatg ccatagccaa tcaccgtcaa
     4081 cagcttgcgt ttcccccaat aatcactcag cacgccggaa aacatcttcg tcagtaaggc
     4141 cgtggcttca gcaaatccct caatgaggcc aaccgacaac agactggcac caagcaccgt
     4201 caccatatag accgggagca ggctgtgaat cagctcggac gacatatcca tacacagact
     4261 caccaggccc agggcccaaa tacttgtcgg gattcgtggt cgatcggaat gagaagaatt
     4321 gggagaagat acaggatcaa ccatagcaga gcttagagtg ttctattgca tactggaagg
     4381 gaaggtcttg attgatcatc cggcaggagg cacctgaggt catcgccctg ataattgggc
     4441 aacacacgcc gtatgcatgg cttccacttc atgtcttgag ggtcccaaga ataccatgtt
     4501 acagcacagt tgttcttggc agaatccata gggaatcatc ccccggccgg tgcgaggatt
     4561 gaacagcctt gcgtcaaacg cattgcgaaa ctctcgaaaa gacacggtct gaattctggc
     4621 aagcatcgaa aaaaaagccc caatcacagg gaaaccgcgg tccatttgac gcacgttgag
     4681 atgcgccatc aaaaaagttg gatagacgga tgcattcgac ctggcgttaa tctctgcaag
     4741 aaaccaccgt cttcttccgg tccggagatc cgcatgttcc acaaaatcaa aaccgaccaa
     4801 ccctgtgtag ccatcttcct gcagccatcg tcccaatgct tcagcagccc gactcatgtc
     4861 attcagcata tgtgccccgg agggggagac atttccaata tgtcccagat gaggattcaa
     4921 gcgctgatcg gatattccga ccacgttcac cgccccccca tcaggctgga catcgacaag
     4981 aatattaggg gagaaggcga cctcatataa tacctgcacg agatagaccg gtgataaggg
     5041 ccttcgcgag atgagttcca atgcctgtcg ctcgctctcg ggattatcct cgacaaggaa
     5101 gatcccggtt ccgccccttc cttcggcgga tcgaataatc acccggccgg tttcagcaac
     5161 gtaacgacga atggccgatc gaagcctagt cacattcggg gcttggccat cagacgactc
     5221 aacaatttcg ccacgggcaa ccggaatacc aagctcctga gcctttcgga acgccacgtg
     5281 tttgagattc gccgcctcgg tcacctcagg actgccgccc atgcacttca cggtcctgcc
     5341 aaggaggttc tccaacgcct gggccatcac ataccccttc tgcgacccgg catccggatt
     5401 cagaacgacg tgattgtgtg gtgggatccg ctcacaaatt ctgtgtaagg agtcgacaga
     5461 ggggcagaag agatcattgt cgaggcattc gccagatacg gggacgggct caagttcaat
     5521 gatatttccc ggttccggcc cgacacccaa ctcgtacaga aatcccatat aggacaggtc
     5581 aaccttacta cttgtcatac aaaccacatc gtgcggcctg gccatgacga gcgcccgctg
     5641 gtcgtaggcg cggaaattgg caggacgcct ctccaaggga agattctttg cgagtgcgac
     5701 cggatcatgt acaaaaatgt gcgtcacatc aggcccaccg agggtggaaa taatgggtgt
     5761 cattccgcca ttatggcttt ccccggaacg atcgattcca ttatccggca gattctctca
     5821 gacatcagct tccccgcaag ccggtgaccg gcggcgttcc agtggccctt ccccaatgca
     5881 gtgttctgaa atccatgcag gaacacaccg tgctgttcag cataggtggc gagaggttct
     5941 gccaggctca ataactcgat gccttcccgt ttcgccaaag cctggatgcg ccgatcagga
     6001 tacagaagat ccggcacacc aagctgacgg gccaatgcct gtcgatgttg cacgtcggga
     6061 tgaacctggt cgggattcga aaggctcaca agaaggaatc ggcgtccccc atcatcgacc
     6121 tctcgcttca tttgcacgat caatcgttcg gtaatcgtcc aggcattttc ccaggatggg
     6181 tccggctggt cgagatagac cagtagatcg agacgaagag cgggatcttc ccagtccttc
     6241 atctgctgtt gctgagccat ttcttccaga aaggatttta gtgtgtattt ggcattgcga
     6301 aacacctgta gcacacggga gatttcaaag gcttcggcac tcagcggaaa ttgttgaagc
     6361 caaaactccg tcgtgtgatg gaaggaattg tcgaggacga ggcgtccgtc gttcatgatg
     6421 aaatagggga tattggtttt cccgttcagt tctctcaggt tcccacgaat atcgttctct
     6481 gaatagaatg ccagcagaac gagatccgga tcatagggag aagccttatg acgcagcacc
     6541 agtaattcct gggctgtact gaagccggac acaccgaaat tcatggcttc tacctgacgc
     6601 ccggcgaggg agggacaatc tgacaactcg tgttcaagcc tggccccata gatctgttct
     6661 gtcgggacct gcatcgcttc cgtgtaggaa tcgcccagaa gcgcaatccg gaaggtccca
     6721 acaggctttt ccagagggtg ctcacggtcg cgcagcccta aactattgat ccgaatgtag
     6781 gcctcacctt cattagaata aagaccttca gcccccggct tatgagcata gccggtcaga
     6841 ttgtcagcaa taaagaaaga ggggtaggaa attccaaaaa ggtaaagacc gatttcaacg
     6901 aggacaaagg caattccaca tccaatgagg actaatgcga tgtttcctgc cccttttctt
     6961 accacccgct ctcatcccca tcattttttc ctatcatcat tcacaaccga ccttctgaaa
     7021 caaggcgaaa tttctccata tttgcactct acccgtaccg gagcttcagg atgaaaatcc
     7081 aaccagccag aaccaaggtc accatattgg cgatgactaa cggccaatca tgattgatca
     7141 atccatat
//
LOCUS       DATIAL010000313         6014 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1348408, whole genome shotgun sequence.
ACCESSION   DATIAL010000313 DATIAL010000000
VERSION     DATIAL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348408"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="VLA60_09790"
     CDS             <1..2167
                     /locus_tag="VLA60_09790"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSF09696.1"
                     /translation="GNPQFLQPCHGVPVPGGTYLHFLAFDVARAPDGKWWVLRDRTEA
                     PTGAGFALENRIIVSRSLPNLFARTQVQRLSSFFQTFSESFLQFSQREDPLAVVLSPG
                     PGNMAYFEHAYLARYLGYPIVEGSDMTVRDERLFLKTVDGLKPVDLVLRRIYSDLCDP
                     LELMTESTTGIPGLLQAIRAGHVTLANALGSGLIESEVLLSFLPTLSKFFFGEGLKIP
                     SVATWWCGQANERAYVLDNMNRLLIRRVLTPKRGLTHDRLSSFGPDLNEQDREALAQA
                     IARRGHEYVGREIVSPSTTPFWTSHDELTPVPMTVRIYLTATKDGYSVMPGGLARVSV
                     RSDPRAHWHEPGDFSKDTWVKSNGPLDIPAAVTLPHHTLQLRRGGRDLPSRTADNLFW
                     LGRYTERAEGAIRLLRSLVSRMSGEAGIGDDPETLHRLVSLLVMQKHLSPRRAKRAVE
                     GGASAVEAELRTILFDPDSPDGLATILQNVRRTAELVRHRLSLDTWNILMELTSVPQD
                     WAQTKGQSIDDAIRLLSRMIQHLAALNGMVMENMTRSYAWRFLEMGRRLERVRHLSKL
                     IRHLASRGTPETTGALNLLLELADASITYRTRYKAEAKLAAVLDLLLADDTNPRSIIF
                     QLLTIESHINALPREEASASINPAQRITTRGCTDIRLADMMELAQFTTKTGVRVNLER
                     LLKQLDQHVHQLSDVVAHTFFSHSLAQRISGPQWLEGIP"
     gene            2164..3054
                     /locus_tag="VLA60_09795"
     CDS             2164..3054
                     /locus_tag="VLA60_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSF09697.1"
                     /translation="MMYQVTHRTSFAYTQPVAISHHVLRLTPRSHPRQHCLRSTVTLD
                     PTPSVRSEGEDYFGNPMTHLTIQTPHPQLIVEAKTVVEVLKPEPIPLDQSPPWEQVVQ
                     QLQSPLDSPNLEAQQFMYDSPYITIDDATYDFVRECFPSGRPLLAGVMELTSRIFEEF
                     TYEGGVTEVSTPVQDVLISRKGVCQDFAHLEIAALRSLGLPARYVSGYLLTHPPEGQE
                     KLVGADASHAWVAAWSPGLGWVDFDPTNNIIPSDEHITLAWGRDYGDVSPINGFMVGG
                     GQHTLAVSVDVSPAPHPSLV"
     gene            complement(3343..4467)
                     /locus_tag="VLA60_09800"
     CDS             complement(3343..4467)
                     /locus_tag="VLA60_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF09698.1"
                     /translation="MALFLMSKKFGWPDGLSDRSIIQQGVNMKLGRSKFLKGVMLFGL
                     IVVLSTPAMAADDLFPSVDQQVPHYSPQSRVSGKVEIDGSTTMKTILETWKDKLEKIH
                     PDLEISLKTDGSNTGVESLMSGKTKIAAMSRPMTKQEIEKFTKQVGYAPTAIPVAVDA
                     FAIFVHKDNPLDHITLQQLDALFSSDRRRGAPESIDTWGQLGLSGVWEKSSIVSHIRD
                     AKSGTGQFFREFVLLDGKDKEMSIVQPGAASVVHAVMNDPYAVGYSGIGYRTNSVKPL
                     HVAAVDGDAFVEPTFQSATNGTYPLHRLLYLYVNEPPDMEHAPLLSEIVKFAVSLEGQ
                     QVVAKSGFFPLPTKDLIAVSATWTRPLDSVSNTKGPKDFK"
     gene            4767..5066
                     /locus_tag="VLA60_09805"
     CDS             4767..5066
                     /locus_tag="VLA60_09805"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HSF09699.1"
                     /translation="MKIKNTAPSCASKLKVLSDPTRLAVLESLMSGQKNVGELMEQLE
                     VEQSLLSHHLSVLRDNGLVEATREGKTMIYKLPENVADSTSGKAINLGCCKISFD"
     gene            5114..5941
                     /locus_tag="VLA60_09810"
     CDS             5114..5941
                     /locus_tag="VLA60_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF09700.1"
                     /translation="MVVSLLATIGVIALCTICSLDASLAQAEGHKLVITGSSTMAPLV
                     AEIGKRFETRHPGVRIDVQTGGSSRGIADVINGVADIGMASRALNSQEQHLHGSVIAQ
                     DGITIILHRSNPVRELTDDQIRAIYTGGITHWNQVGGSDAPITVVNKAEGRSTLGLFL
                     DYFQVSNRDIHPHVVIGDNEQGIKTVAGNPHAIGYVSIGTAQYDATHGIPIRLLPLQH
                     IPATIGTVREGMFPLSRPLTLVTKSPPSGLIKTFIDFARSSQANDLILKQYFVPLQR"
BASE COUNT         1423 a         1662 c         1427 g         1502 t
ORIGIN      
        1 cgggaatccc cagtttttac aaccctgcca tggcgtaccc gtgccaggag gcacctattt
       61 acattttctg gcatttgatg tggcgagagc gccggacggg aaatggtggg tcttgcgtga
      121 tcgcaccgaa gcacccaccg gtgccggatt cgctctcgaa aatcgcatta ttgtctcacg
      181 gtctttaccc aacctgtttg cccgaactca ggtccaacga ctctcttctt ttttccagac
      241 cttcagcgag agtttcctcc agttttccca acgtgaagat cccctggcgg tcgtgctctc
      301 gccgggcccg ggaaatatgg cgtattttga gcatgcctat ctggctcggt atctgggcta
      361 cccgatcgtc gaagggtcgg atatgaccgt acgcgatgaa cggctgttcc tgaaaacggt
      421 cgatggtctt aagcccgttg atctggtctt acgccggatt tattcggact tgtgcgaccc
      481 tctcgaattg atgaccgaat ccacaacagg tattccaggg ttactccaag ccattcgagc
      541 cggacacgtc acattagcca atgcgctcgg aagcggattg atcgagagcg aagtcctctt
      601 aagctttctg ccgacattat ccaaattctt ttttggtgaa ggacttaaaa ttcccagtgt
      661 cgccacctgg tggtgcggac aggccaacga gcgcgcctac gtgctcgata atatgaaccg
      721 attgttaatt cgtcgtgtct tgacacccaa gcggggcctc acgcacgacc gtctctcctc
      781 cttcgggccc gacttgaacg agcaggaccg tgaagctctg gctcaagcca ttgcccggcg
      841 tgggcatgaa tacgtgggac gggaaatcgt ttcgccatcc accactccct tttggacctc
      901 ccacgatgag ctcacaccgg tcccgatgac tgtccgcatc tatctgacag ccacgaagga
      961 cggctattcc gtcatgccgg gtggactcgc tcgcgtgtcg gttcgatcgg atccacgcgc
     1021 gcactggcat gaacccggtg atttcagtaa agacacctgg gtgaagtcga acggtcctct
     1081 tgatattccg gcagccgtca cgcttcccca tcatacgctt cagcttcggc ggggaggacg
     1141 ggatcttccc agccggaccg ctgacaatct cttctggctg ggacgttaca ccgaacgcgc
     1201 tgaaggtgcg atccgtttat tacgaagcct ggtgtcacgc atgagcggag aggcgggaat
     1261 cggagatgac cctgagaccc tgcatcgctt ggtttcactc ctcgtgatgc aaaaacattt
     1321 atcgccccgt cgcgccaaac gggccgtgga aggcggagcc agcgcggtgg aggcggaact
     1381 tcgaacgatt ttatttgacc cggattcgcc ggacggcttg gcaaccattc tccagaatgt
     1441 acgacgcaca gcagaactgg ttcgccaccg gttgtccctc gatacctgga atattctcat
     1501 ggagctcacc agcgtgccac aggattgggc acagacaaaa ggacaaagca tcgacgatgc
     1561 gattcgactg ttgagccgga tgattcaaca tctggcagcg ctcaacggca tggtcatgga
     1621 aaatatgacc cgcagctatg cctggcgttt tctggaaatg ggacggcggt tggaacgcgt
     1681 gagacattta tccaaactca tccgccattt ggcatcacga ggcacgccgg aaacgaccgg
     1741 cgccctcaat ctgctgctgg aattagccga cgcctccatt acctatcgaa cccggtacaa
     1801 ggctgaagcc aaactcgctg cagtcttgga tttgcttttg gctgatgata ccaacccccg
     1861 ttcaattatt ttccagttat tgacgatcga gtcacacatc aacgcattgc ctcgggagga
     1921 agcctctgct tccatcaacc cggcacagcg gattaccaca cgcgggtgca cggatattcg
     1981 actggccgac atgatggaac ttgctcaatt cacgaccaaa accggcgtgc gagtcaatct
     2041 tgaacgacta ctcaaacaac tggatcaaca tgtgcaccaa ctctccgatg tggtggctca
     2101 cacctttttc agtcattcat tggcccaacg gatttcaggg cctcaatggc ttgagggcat
     2161 tccatgatgt atcaggttac acaccggacc tccttcgcct acacccagcc ggttgccatt
     2221 tctcatcatg tcttgcgtct cactccacgg tcccatcctc gacaacattg ccttcgatcc
     2281 accgtgacat tggatcccac gccatcggtc cgttcagaag gtgaggatta ttttggcaat
     2341 cccatgactc acctgaccat tcaaaccccc catcctcaat tgatcgtcga agccaaaacc
     2401 gtcgtggagg tcctcaagcc ggagccgata ccgttggatc aaagtccgcc ttgggagcaa
     2461 gtcgtccaac aactccaaag tcccttggat tcgccgaatc tggaagccca acaatttatg
     2521 tatgattctc catacatcac cattgacgat gccacgtatg actttgttcg tgaatgtttt
     2581 ccatccgggc gtccgcttct ggccggggtt atggaattaa ccagccgaat ttttgaggaa
     2641 ttcacatacg aaggaggtgt gaccgaagtg tccaccccgg ttcaagatgt cctcatctca
     2701 cggaagggcg tttgtcagga ctttgcccat ttggaaattg ccgcactccg cagccttggt
     2761 ttgcccgcac gctatgttag tggatacctt ctgacgcatc cacctgaagg acaggaaaaa
     2821 ctggtgggcg ctgatgcctc acatgcctgg gtagccgcat ggagtcccgg cctcggttgg
     2881 gtcgactttg atcccaccaa caatatcatt cccagcgatg aacacatcac cttggcctgg
     2941 ggaagagact atggggatgt cagtccgatt aatggcttca tggttggggg cggacagcat
     3001 acgctcgccg tatccgtgga tgtcagcccc gctccacatc cttcactcgt gtaatcccga
     3061 ttctgcaaca atacgaattt catttttcat tcgagaaacc ctgcctcagt ttctatcaga
     3121 gttcccactc ccattccctc tggacaaaaa taatgccttc cggacttgcc tatgatgaga
     3181 caagaccctc tcattctatt gtaacgagag cgtcatccct tttatgctgt ctggcatcag
     3241 gctccttgtc gggtcctgac tcccagcgtc tgcaaggcca tgtaagaacc tcagaggaag
     3301 tacggcatgc ttgctgccgg aaaatttccc cacatagtcg gactatttga aatccttcgg
     3361 tccctttgtg ttagagaccg aatccaacgg cctggtccag gttgcggaca cagcaatcag
     3421 gtctttggtt ggaagtggaa aaaatcccga cttcgctacc acttgttggc cttccaaact
     3481 cacggcaaat ttaacgattt cggagaggag aggagcatgt tccatatccg ggggctcatt
     3541 cacatagagg tataacaacc gatgaagtgg atacgttcca ttcgttgcgg attggaatgt
     3601 cggttcgaca aatgcatcgc catcaacggc agccacatgt aagggtttca cagaattggt
     3661 gcgatagccg attccactat agccgacggc ataaggatca ttcatgacgg catgaacaac
     3721 cgaggcggca ccaggttgaa caatactcat ctctttgtct ttcccatcca acaagacgaa
     3781 ttccctgaaa aactgtccag ttcctgattt cgcatcccga atgtgagaaa caatggaaga
     3841 tttttcccat accccggaaa ggcccaactg tccccacgtg tcgatggact ccggagcccc
     3901 tcggcgcctg tccgaggaga acaacgcatc caattgttgt aatgtaatgt ggtcaagtgg
     3961 gttatcctta tgaacaaaga tagcaaacgc gtcgacagca acggggatcg ccgtgggtgc
     4021 ataaccaacc tgtttggtga atttctcgat ctcttgcttc gtcatcggac gcgacatggc
     4081 cgcaatcttc gtcttcccgc tcattaacga ttcaacgccc gtgttggatc cgtccgtctt
     4141 cagactgatc tctaaatcag gatgaatttt ctccagcttg tccttccatg tttccaaaat
     4201 cgttttcatg gtagtggacc cgtcgatttc gacttttcct gacacacggc tttgtggaga
     4261 atagtgggga acctgttggt ccacactggg aaacaagtcg tctgctgcca tggcaggagt
     4321 actcagcact acaatcaacc caaacagcat cacaccctta aggaatttac ttctccccaa
     4381 tttcatattc actccttgtt gaatgattga ccggtctgat aggccatcgg gccaaccaaa
     4441 tttctttgac atcaggaaga gcgccatccg tctcatcctt cccctatcat ccctttaggt
     4501 caaaatttac cgctaacgta tgtcagccaa atgtcacgca tgttacaaat gagttaattt
     4561 tttcctggaa cccatattgt tcataaagag agaagtgaac gacccagttt actcaggtca
     4621 ttttgaatac attcgggcgg aaaacaagac ctcccagacc taccgtgcca attgatcttg
     4681 acgcataaca accgtttata ttaaatactg ttcatatgaa tatatatcca tttatttagg
     4741 ctttccattc actattttcc cgtgcaatga aaatcaaaaa tactgctccc tcctgtgcaa
     4801 gcaagctcaa ggtgctatca gatccaacac gcttggctgt tcttgaatcc ctcatgtccg
     4861 gacaaaaaaa tgtcggggaa ttgatggaac aattggaggt ggaacaaagc ttattatccc
     4921 atcatttaag cgtgttgcgg gataacggcc tggtggaagc cacacgggag ggaaagacca
     4981 tgatctacaa actgccggaa aatgtcgccg attctacgag tggtaaagcc atcaatcttg
     5041 gatgctgcaa aatctctttt gattaagatc gtcctgtgtt ttcttgaaaa aaccactgtt
     5101 gaaaaacttt tgtatggtcg tctcccttct tgcaaccata ggtgtcatcg cactctgcac
     5161 gatatgttcg ttagatgcct cgcttgcgca ggctgaagga cacaagcttg tcatcaccgg
     5221 atccagtacc atggctcccc tggtggcgga aatcggcaaa cgatttgaga cacgtcatcc
     5281 gggtgtccga atagacgtcc aaacgggagg ttcttcacgc ggcattgctg atgtcatcaa
     5341 tggagtcgct gacattggca tggcatctcg tgctctgaac tctcaggaac aacatctgca
     5401 tggatccgtc attgcccaag atggcattac cattatcctg catcgatcaa atcctgttcg
     5461 ggaattaacc gatgatcaaa tcagggctat ctataccgga ggaatcaccc attggaacca
     5521 ggttggcgga tctgacgcac ccattaccgt cgtgaataaa gcagaagggc gttccacctt
     5581 gggattattt ctggactatt tccaagtatc caatcgggac attcaccctc atgtggtcat
     5641 cggagacaat gaacaaggta ttaaaaccgt ggccgggaac ccccatgcga ttggatatgt
     5701 ctccattgga acggcacagt atgatgccac ccacggcatt ccaatccgat tattaccgtt
     5761 gcaacacatt cccgccacga ttgggacggt cagggaaggc atgtttcccc tctcccgtcc
     5821 cctgaccctt gtaacaaagt ctcctccttc aggactcatt aaaaccttta ttgatttcgc
     5881 gcggtcctct caggccaatg atctcatcct caaacaatat ttcgtcccgc tccaaaggtg
     5941 atttcctgct gtcggggagt gtgcgcctcc tcgcgttgat ggctggtgga ctcttggtcc
     6001 tggtcgtatc attt
//
LOCUS       DATIAL010000314         7522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1133164, whole genome shotgun sequence.
ACCESSION   DATIAL010000314 DATIAL010000000
VERSION     DATIAL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7522
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133164"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1375)
                     /gene="xseA"
                     /locus_tag="VLA60_09815"
     CDS             complement(32..1375)
                     /gene="xseA"
                     /locus_tag="VLA60_09815"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HSF09701.1"
                     /translation="MPGLFPSALTVSTLTRQIRSTFEQNFPPLWVEGEISNLRCPSSG
                     HRYFTLKDQSSQIRAVLFRSQVERLKFSLQEGLEVFVFGRLSVYEPRGDYQLLLEVVE
                     PKGVGALQLAFIQLKAKLEAEGLFEASRKRMLPLYPHRIGLVTSPSGAAIHDLLTIIH
                     RRWPFAQILIAPVPVQGDEAAGQIAKAIGALNQLGELDVLIVGRGGGSLEDLWAFNEE
                     IVVRAIASSRIPVVSAVGHETDLTLADLAADYRAPTPSAAAELVVPDCSTVRHHLGHQ
                     RVRLERSIRSLCVAWRGQVQELTGRLPEPRLVLGQFVQQVDELERQLYVKMKHWCRNL
                     QVQLLKHQSSIWERNPLIDIHRQQLALVDRGIRLVRGMSGCILDKRHQTELWMSQLNQ
                     LSPLAVLARGYSIVHKLQNGKVVKQATEVSEGESVRARLHEGELICLVQRTHPST"
     gene            complement(1385..2164)
                     /locus_tag="VLA60_09820"
     CDS             complement(1385..2164)
                     /locus_tag="VLA60_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HSF09702.1"
                     /translation="MNVLFIGDIMGEPGRRVILKHLSNVIQEHQIDLVIGNGENAAGG
                     FGITQEVAEDLFDLGLSVITLGNHAWDKREALEYLQKEQRVIRPANYPDGVPGKGTCV
                     VETIHGERLGILQLMGRVFMPMVDCPFRVAERELAQLTTQTHCILVDMHAETTSEKMA
                     MGYFLDGKVSAVLGTHTHVQTADEQILPQGTGYLTDVGMTGPVQSVIGMKPNLVIQKF
                     LTQLPKRFEVATGPSVLSAAVLNLNYSTGKTTHITRLRVFE"
     gene            complement(2185..3747)
                     /gene="rny"
                     /locus_tag="VLA60_09825"
     CDS             complement(2185..3747)
                     /gene="rny"
                     /locus_tag="VLA60_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HSF09703.1"
                     /translation="MDTVIPILAVVIGLFLGFVLSKVYQQQRDLKRRSAAEEQAQQLT
                     QNAQREAENLVKEAKIEVKDLLFQAKSELETKEKEKRNEIQAADRKIIQREEALERKI
                     NQFEKRDEEMRRKERSLKEKEEALVNKAAACDQAIKEHRLALEQVAGITAEEAKRQLL
                     GEIESEARMDAALLTKRILDEAKETAEREAREIVTRSIQRITRDYVNEATISVVPLAN
                     DSMKGRIIGREGRNIRALEAATGVDLIIDETPEAVIVSGFDPLRREVAKIALERLMQD
                     GRIHPTRIEEVVEKVKGDLDKLMRDDAEKVIFEVGLSDFHPEIVKLLGRLKYRTSYGQ
                     NNLYHAREASYICGIMAAELGLDVKLAKRGALLHDIGKVVSHEEEGTHAMLGAELAKK
                     YGESELIVNAIAAHHEQVEPLCPESVLVAAAEALSAARPGARRETLEAYVKRLEKLES
                     LATGFTGVDKAYAIQAGREIRVIIRQGELNDSESFALSRDLAKKIEQELTYPGQIKVT
                     VIRENRFIEFAK"
     gene            complement(4455..4748)
                     /locus_tag="VLA60_09830"
     CDS             complement(4455..4748)
                     /locus_tag="VLA60_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HSF09704.1"
                     /translation="MTRTIEVEIFGHRISLQGEGDEAYFHELAGYVDAQMRTLAQKTR
                     TSTPTKLAILAAINITDQLFRQERHRQFGEAEIERRTQVLLETIEKHLAANPH"
     gene            complement(4765..4998)
                     /gene="zapB"
                     /locus_tag="VLA60_09835"
     CDS             complement(4765..4998)
                     /gene="zapB"
                     /locus_tag="VLA60_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HSF09705.1"
                     /translation="MSVEKLETLEVRVKKLLDLVIELRQDKSHLEQELESTRERLAKH
                     EEMSEGWEEERTTIRSRIEKVLGELEFLDRSND"
     gene            complement(5048..7300)
                     /locus_tag="VLA60_09840"
     CDS             complement(5048..7300)
                     /locus_tag="VLA60_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSF09706.1"
                     /translation="MKILSIHVIGGVIVGGCILFLPQTVVSLDPPSAPCQNPEGCVAQ
                     FLEQLESSGTGSGTRLDHAKFFNEFVAANGNSPLAKHVGIRYGYWLKESFPLEAIPLL
                     QASLRDFPILRDYLTFWMGQAYAHAGLGKEAAEAFQQFSEHFSDSLLRADALYAGGDV
                     VAKLGDCEAALSMWSQALSIKSDHPKSANAFFQMGLCSAQMGQREKAIEIFRELWWKF
                     PLAQERVQAESWLRREVGSLFLPSLEERFKRSMALYNGGALEEAVQEFQHISSLSPPA
                     PQLFQVQYTLAMALVRLKRYDQAETILTSLSRSSSSRRDDAWVWLGRVYLRQGKGPEL
                     EALVGARSNEKVTGDQQSLLLTFYGIWLEDHARWLDAGKAYKRAAAVAHSLTQRLDAL
                     WRVGWIHYQQKQFIEAIEMFQEIIQAVGTPQTDSSLHAASQAFYWLARSQEHLGQMEP
                     AREGLKNLSQDYPFTYYGQLAEIRLGPTEFSTKQWAVLASTDMMNVETPAHLQQDIHY
                     QKTLELRTLRLYKEAVQELEAAFARFGADPKAFPQLVSLASEVGAYDVGIRLSIRHFG
                     GTLRKGQLHSLSAAWLGAFPMGYQSVIQSFAPKHVDPFLVAGLIREESLYSARVVSPV
                     GAIGLMQLMPETAKKVGRQLGLQDSDSDRKGLDEPNRNIQLGTYYLGQLLNEFQGNII
                     YAVAAYNAGPQAVKRWIGQNGHRDPDEFIELIGYRETRGYVKRVLGSYRIYRTLFGDA
                     CRGVSLDRFC"
BASE COUNT         2005 a         2015 c         1601 g         1901 t
ORIGIN      
        1 catggcggaa catgaagcaa aagggaattc cttacgttga aggatgggtg cgctgtacca
       61 gacaaatcaa ttcgccctca tgaagtctgg cgcgtacaga ctctccctca gaaacttcgg
      121 ttgcttgctt caccactttt ccattttgca gcttgtgaac aatgctatac ccccgagcta
      181 agaccgcaag gggacttaat tgattcagct gagacatcca cagctctgtt tgatggcgtt
      241 tatcaagaat gcaccccgac atacctcgta caagccgaat ccctcggtca accaaagcca
      301 gttgctgacg atgaatgtca atcaggggat ttctctccca aatcgaggac tgatgtttca
      361 gcaactgcac ctggagattc cggcaccagt gcttcatttt tacatagagt tgccgctcta
      421 actcatccac ctgttgcacg aattgtccaa gcacgagtct aggctcaggc aatcgtccgg
      481 tcaattcctg aacttgtcct ctccacgcga cacataaact ccttatggac cgttccaatc
      541 gtactcgttg atgccccaga tgatgcctaa ccgttgaaca atccggaacc accaactctg
      601 ccgcagcgga gggagtggga gcccgataat cagcagctaa atctgcgagc gtcagatcgg
      661 tttcatggcc aacagcagac accaccggaa tcctggaaga ggcgatagcc ctcaccacaa
      721 tttcttcatt aaatgcccag aggtcctcca acgatcctcc accacgcccc acaattaata
      781 catccaattc acccaattgg ttgagcgccc cgatagcctt agcgatttgt ccggcagctt
      841 catccccttg cacgggaaca ggagcaatca gaatctgtgc aaaaggccaa cgtcgatgaa
      901 tgatggtgag cagatcatgg attgcagcac cggaaggtga cgtcaccaga ccaatccgat
      961 gcgggtaaag aggaagcatc ctttttctcg aagcttcaaa taatccttcg gcttccaact
     1021 ttgccttcag ttgaataaag gctaattgga gcgcaccaac gcccttgggc tcaaccacct
     1081 ccaagagtaa ttgatagtct cctcgtggtt catacaccga aaggcgaccg aacacgaaaa
     1141 cttccaaacc ttcctgaagc gaaaatttca ggcgttcgac ctgactgcgg aagagcacag
     1201 ctctgatctg actcgattgg tctttcaggg taaaataccg gtgcccagaa gacgggcagc
     1261 gaagattaga aatttcccct tcaacccaga gtggaggaaa attttgttcg aacgttgaac
     1321 gaatctgtcg ggtgagagtc gagacggtaa gcgctgaagg gaaaagccct ggcacagaag
     1381 taagttactc aaacacacga aggcgcgtga tgtgagtggt ttttccagtt gagtaattaa
     1441 gatttaacac tgccgcactt aaaacagaag gtcctgtcgc cacttcgaat cttttcggaa
     1501 gttgggtcag aaatttctga atcaccaagt ttggtttcat tccgatgacg gattgaacag
     1561 ggccggtcat cccgacatcg gttaaatacc ccgttccctg gggtaagatt tgttcatcag
     1621 cagtttgaac atgcgtatgc gttccaagca ccgcagacac cttgccgtcc aaaaaatacc
     1681 ccatcgccat tttttctgag gtggtttcgg catgcatatc cacgagaata cagtgagtct
     1741 gtgtcgttaa ttgagccaat tcgcgttccg caacccgaaa agggcagtcc accatgggca
     1801 taaacactcg ccccataagt tgcagaatcc ctaggcgctc cccatgaatg gtttctacga
     1861 cacacgtgcc ttttcctgga actccatcag gataattcgc gggacggatc acgcgctgct
     1921 ctttttgcag atactctaaa gcttcacgtt tatcccaggc atgattgccc agtgtaatga
     1981 ctgaaagacc caaatcaaac aagtcttcag ccacttcctg tgtgatgcca aacccacctg
     2041 ccgcattctc cccgttgcca ataaccagat caatctgatg ctcctgaata acatttgaca
     2101 gatgctttaa gatcacccga cggccaggct ctcccatgat atctccgata aacagtacgt
     2161 tcatgatctc ctttgctcac agagctattt ggcaaattcg ataaagcgat tttcccgaat
     2221 gacggtcact ttaatctgcc cagggtaggt cagttcttgt tcaatctttt tggccaaatc
     2281 ccgagacaga gcaaaacttt cactgtcgtt caattctcct tgacgaatta tgacccgaat
     2341 ttcacgccca gcctggattg cataggcctt atcaacccct gtaaaccccg tagctaacga
     2401 ttccagcttt tccaatcgct tgacataggc ttcgagggtt tcgcgtctgg cacccgggcg
     2461 ggcggccgag agagcttccg cagcagccac taataccgat tcgggacaca gcggctcaac
     2521 ttgttcatga tgggcagcaa tggcattcac aatgagctcc gactctccat acttctttgc
     2581 caattccgcc ccgagcatcg catgggtacc ctcctcttca tggctgacca cttttcctat
     2641 atcatgcaat agggctcctc gcttagccaa tttcacatct aaccccagct ctgcagccat
     2701 aatcccacag atatatgacg cttccctggc atgatataag ttattttgtc cataactggt
     2761 tcgatatttc aggcgaccca ataatttgac aatttcaggg tgaaaatctg aaagcccgac
     2821 ttcaaagatg actttttcag catcgtctct cattaacttg tctaagtcgc ctttgacctt
     2881 ttcaacgact tcctcaatcc gagtcgggtg aatacgccca tcctgcatga ggcgttcaag
     2941 cgcaattttc gcgacctccc tccgaagggg atcgaatccg gacacgatca ctgcctccgg
     3001 ggtttcgtca ataataagat caacgccggt tgccgcctcc aaagcccgaa tatttctacc
     3061 ctcgcgaccg ataatccttc ccttcatgct atcattcgcg agaggaacca ctgaaatcgt
     3121 ggcctcgttg acgtaatctc ttgtgatccg ctgtatcgac cgggtgacaa tttctctagc
     3181 ctctcgttca gcggtttctt tagcctcatc caaaattctt tttgttaaca acgccgcatc
     3241 cattcgtgct tcactttcaa tttcacccaa caactggcgc ttggcttcct cggccgtaat
     3301 tcctgccacc tgctccaggg caagccggtg ctccttaatg gcctggtcgc acgcagcagc
     3361 tttattgacc agagcttcct ccttttcctt aagggaacgc tcttttcggc gcatctcctc
     3421 atcacgtttt tcaaattggt tgatcttacg ctcgagggcc tcttctcgct ggatgatttt
     3481 tcgatccgcg gcttgaatct cattcctctt ttccttttct tttgtttcaa gttcagactt
     3541 ggcttgaaac agcagatctt ttacttcgat ttttgcttct ttgaccagat tttcggcctc
     3601 tcgttgagca ttttgggtga gctgttgagc ttgctcttca gctgctgacc gacgcttcaa
     3661 gtccctctgt tgctgataca ccttcgataa cacaaatcca agaaaaagcc cgatcaccac
     3721 tgcaagtatg ggaattaccg tgtccattgg ttagctccta ttcgtttcct catgtcacaa
     3781 acacgagaaa gatttatctt ccctctgtta ggcggaaaca aacccgcaaa accacagggg
     3841 aaaatcagaa aaggaaggaa aacaaaaact ggagaaagcc tatctgatag gacagaaggc
     3901 ccatatttgt aggaaacgag gaggaggaga gatagggcaa ctctctaagc caaaagagcc
     3961 cataacaaca cgcaaaggac tcacccaatg aatccagtct tcacagtttc tgcctgaaat
     4021 agaagaacct ggataccaca caggccaaaa atttaaaatt tttagatcaa aaggcgagcc
     4081 atttggatag aaatgagcaa tttgcctcgt aaacaacgat ttccaagaat tcaacctgcc
     4141 cttaaataaa atttccctac ctcgtacgat cccatcatac aaaaaaccca caatgactat
     4201 gaaaatactt gtcatagtgg gcctccagtt aaattccctc cacaagaggg atattccttt
     4261 ctttctttga aattcaataa cttatgaaaa gtaaaatatc agaagacgat gaggaatttc
     4321 aataatgtgc cagttgaatg atgggaatag acggacaaca ttcggccaac caatggaatg
     4381 aagcacttca accaattgat ttcaaaggta atacctaatt aacgctggac aagaggaaat
     4441 gaaggagaga atggttaatg cgggttggct gcgaggtgtt tttctatcgt ttccagtagg
     4501 acttgagtcc gtcgctcaat ttcagcttct ccaaactgtc ggtgtctctc ttgtcgaaag
     4561 agctgatccg taatattaat tgcggccaaa atagccagct ttgtgggagt gcttgttcgg
     4621 gttttttggg cgagggtacg catttgcgca tcaacatacc cggccaactc atgaaaatac
     4681 gcctcatctc cctctccctg gagagagatt cgatgaccaa agatttcgac ttcaatggtc
     4741 ctcgtcattc ttaagtcctg tagattaatc gtttgaacgg tctaaaaatt caagctcgcc
     4801 cagcactttt tcaattctgg atcgaatcgt ggttcgctcc tcttcccatc cttcagacat
     4861 ttcttcgtgt ttggccagcc gttcccgagt cgattccaac tcctgttcaa gatgggattt
     4921 atcctgccgt agttcaatga ccagatcaag taatttctta actcgaacct ccaacgtttc
     4981 caatttctct acgctcacgt gcacgcctcc ttattcgcaa ggtcaaacga ggcggactat
     5041 aattcgttca acaaaacctg tcaagagaaa cacctcggca ggcatcccca aacaatgtcc
     5101 gataaattcg ataacttcca agtacgcgtt tcacatatcc acgggtttct cgatatccaa
     5161 tcagctcgat aaattcatcc ggatctcgat gaccattttg cccaatccac cgtttgaccg
     5221 cctgaggtcc agcgttatac gcggctactg cataaataat attgccctga aattcattca
     5281 gtaattgccc caggtaatac gtccccaatt gaatattgcg atttggctca tccaggccct
     5341 ttcgatcaga atcggaatcc tgtaatccca attgacgacc aacttttttg gctgtctctg
     5401 gcatgagctg catcaggcca atcgcgccaa ccggggagac aacacgagca ctatataggc
     5461 tttcttctcg tataagcccc gcgactaaaa acggatccac atgctttggg gcaaacgatt
     5521 gaatgacact ttggtagccc ataggaaaag ctcccaacca ggcggcagac aaggaatgga
     5581 gctgtccttt gcgcaacgtt ccgccaaaat gacgaataga caatcgaatc ccgacgtcgt
     5641 aggccccaac ttcgcttgct aaggagacca attgaggaaa ggctttggga tccgctccga
     5701 agcgggcaaa agcagcctca agctcttgga ctgcctcctt atataggcga agggttcgta
     5761 actctagagt tttttgataa tggatatcct gctgaagatg agctggtgtc tccacattca
     5821 tcatgtctgt tgatgccaac actgcccatt gtttcgtcga aaattctgtg ggaccgagtc
     5881 gaatctccgc caattgcccg tagtaggtaa aggggtagtc ctggcttaaa ttttttagac
     5941 cttctcgcgc gggttccatc tgcccaagat gttcctggga acgtgccagc caatagaaag
     6001 cctgagaagc cgcatgcaga gaagaatccg tttgaggagt tccaactgcc tggataatct
     6061 cctgaaacat ctcaatggct tcaatgaatt gtttctgttg ataatgaatc catcccactc
     6121 gccaaagcgc atccaaccgc tgcgtcagcg aatgagccac cgccgctgcc cgtttatagg
     6181 ccttccctgc atctagccat cgcgcatgat cttccaacca aattccgtaa aaggtgagga
     6241 gcaacgattg ctgatcccct gttacttttt cattcgaccg ggctccgaca agagcctcca
     6301 attctggccc ttttccctga cgcaaataca ctcttcccaa ccatacccag gcatcatctc
     6361 ttctggagga ggatgaacgc gagagtgagg tcagtatcgt ttctgcctgg tcataccgtt
     6421 tcaaacgaac taaggccatg gccaacgtgt attggacctg aaaaagctgg ggagcgggag
     6481 gagatagaga cgaaatgtgt tgaaattcct gaaccgcttc ttccaaagcc cccccattat
     6541 ataatgccat gctacgcttg aatcgctctt ctagtgaggg taaaaacaaa gaacccactt
     6601 cccggcgtag ccacgactca gcctgcactc gctcttgggc taaggggaat ttccaccata
     6661 attcacggaa aatctcaatc gctttttccc gttgtcccat ctgagcagaa catagaccca
     6721 tttgaaagaa ggcatttgcg gatttgggat ggtcagattt tatactgaga gcctgagacc
     6781 acatggagag agcggcttcg caatccccca atttggccac cacatcccca cccgcataaa
     6841 gtgcgtcagc tcgaagcaaa gaatcggaaa aatgctcact aaactgttga aatgcctctg
     6901 ctgcttcctt ccccaaacca gcatgggcgt atgcctgccc catccaaaag gtgagataat
     6961 ccctcagaat agggaaatca cgaagagagg cttgcaacag aggaatggcc tcaagaggga
     7021 aggattcctt gagccaatat ccataacgaa tcccgacatg tttggccaaa ggggaattgc
     7081 cattggctgc gacaaactca ttgaaaaatt tggcatgatc caagcgggtt ccagaaccag
     7141 ttccggaaga ttctagttgt tcaagaaatt gcgcaacaca gccttcagga ttttgacacg
     7201 gggcagaagg agggtccaag gagacgacgg tctgaggaag gaaaaggata catcctccca
     7261 cgatcactcc gccaatcaca tgtatcgaca aaatcttcat gactctcctc aatatcatta
     7321 tctaccagct tacccagtat tcgccatgca tacaatcacc agaatgccag aaaatttcaa
     7381 tcagtacagt gatagatcga gtcgtccggg agactgccct tcacccccct caattcgata
     7441 acacgggaag acactcttta gacaaatcct acaccctctt cttggcaatg ggaacccaaa
     7501 gaattccgga attttggacc ag
//
LOCUS       DATIAL010000315         2287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_918542, whole genome shotgun sequence.
ACCESSION   DATIAL010000315 DATIAL010000000
VERSION     DATIAL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918542"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VLA60_09845"
     CDS             complement(<1..461)
                     /locus_tag="VLA60_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HSF09707.1"
                     /translation="MKSLFDHYQMKPGFDEMFESAGVPRRHYRRLVERLRNFSSKDLE
                     LKRRQADQAFLRQGITFTVYGDLQQTEKIFPFDLMPRIIPDLEWRVIEEGIRQRVMAL
                     NMFLVDLYGDQRILNDHVVPRDLIESSVHFQKDLVGFCPPKEVFIHVSGVD"
     gene            749..>2287
                     /locus_tag="VLA60_09850"
     CDS             749..>2287
                     /locus_tag="VLA60_09850"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HSF09708.1"
                     /translation="MALHVALNHQTHYRYDRRVQMGPHVIRLRPAPHSRTPILSYALK
                     IEPTGYFLNWQQDPHGNYLARIIYPEKVQEFHVEVDLVADMVIYNPFDFFLEPDAETL
                     PFNYDPLVKEDLLPYLATEPPGPRLQEWLRQFTAPHDDPTVPFLMSLNQQLKSQIDYV
                     VRMEPGVQNPDETLELGRGSCRDTGWLLVQILRHLGIASRFVSGYLIQLVADVKSLDG
                     PSGTDRDFTDLHAWVEAYLPGAGWVGLDATSGLLAGEGHIPLACTPHPLTAAPISGAM
                     EICETTFSHEMSVTRIVETPRVTKPYTEEQWQAIDQFGQRLDQELAANDVRLTIGGEP
                     TFISIDHPDAPEWNTEAVGETKRPLAAILIKRLRERFAPGGLLHFGQGKWYPGEPLPR
                     WAFSLYWRQDGKPIWQEDQLFAEENQASNISADDANHFIQGIAKRVEVGTKTILPVYE
                     DPWHFIGQERKLPENLEPATNNLDDPMARARLAKVYERGLGKPAGYVLPLQRWNAKDG
                     PRRWV"
BASE COUNT          559 a          634 c          577 g          517 t
ORIGIN      
        1 aaatcaactc ccgacacatg gatgaacacc tctttgggcg gacaaaaacc cactaaatct
       61 ttttggaaat gcacggaact ttctatgagg tcacgaggca ccacgtgatc gttaaggatc
      121 cgttgatcgc cgtacaagtc cactaaaaac atattcaagg ccatgacgcg ctggcgaatc
      181 ccctcttcaa taacccgcca ttcgagatcc gggatgattc ggggcatcaa atcaaaaggg
      241 aaaatcttct ccgtttgctg gagatcgcca tagacggtaa aggtaatccc ctgccgcaaa
      301 aacgcctgat ccgcctgacg ccgcttcaac tccaaatcct tcgaagagaa attccgtagc
      361 cgttcgacga gacgccggta atgacggcgt ggaacgccgg ccgactcgaa catttcatcg
      421 aatcccggct tcatttgata gtggtcaaat aacgatttca caaccctacc cgttgatcag
      481 tggcctcttg gttataagat gatctcaccg aaatttacga tgtagagtgt agcatagccg
      541 gccggcccaa gggcaatttc aacaagagaa tacggaccat acaaagcttg tcctgacgcg
      601 gtgattggat cttttgggga atggttcagg aatggtgcaa aggacgaatg agagtgctaa
      661 gatccgttga ttcgtcatgt gaaaatggtc atttcatttt tccgcttttt ttcaccaaga
      721 caggcattcc ctgggctatt gatttttcat ggcactccac gtcgcactca atcaccaaac
      781 gcattatcgc tacgatcgcc gggttcagat gggacctcac gtcatccggt tgcggccggc
      841 tccccatagc cggactccca ttctgagtta cgcactcaag attgagccca ccggttattt
      901 cctgaactgg caacaggatc ctcacggcaa ctatctggca cggattattt atcctgaaaa
      961 ggttcaggaa tttcatgtgg aagtcgacct ggtggccgac atggtcatct ataatccctt
     1021 tgattttttt ctggagccgg acgccgagac acttcccttc aactatgatc ccctggtcaa
     1081 agaggatctg ctgccgtatt tggccacaga accaccaggc ccgcgactgc aagaatggct
     1141 gcgacagttc accgcgccac acgatgatcc taccgtaccc ttcttaatga gtctcaatca
     1201 gcaactgaag tctcaaatcg actatgtcgt ccgcatggaa cccggcgtgc aaaaccctga
     1261 tgagacgctg gaactgggcc gtggatcgtg ccgggacacc ggttggctcc tcgtgcagat
     1321 ccttcgccat ctgggcattg ccagtcgatt cgtctccggc tatttgattc agcttgtcgc
     1381 cgacgtgaaa tccttggacg gtccgtccgg taccgatcgg gactttacag accttcacgc
     1441 ctgggtagag gcatatctgc cgggggctgg atgggtggga ttggatgcca cgtccggcct
     1501 gttggctgga gaaggtcaca tcccgctggc ctgcacacct caccccctga ctgctgcgcc
     1561 gataagtgga gcgatggaga tttgtgaaac aacgttctct catgaaatgt cggtcacccg
     1621 tattgttgaa acgccacggg tcaccaagcc atacacagag gaacaatggc aggcgatcga
     1681 tcaatttgga caacgactgg atcaggaatt ggcagccaac gatgtccggc tcacaatcgg
     1741 aggagaaccc acctttatct ccatcgatca tcccgatgct cctgaatgga ataccgaggc
     1801 tgtcggtgag acgaaacgac cattggcagc gattctcatc aaacgcttac gggagcgttt
     1861 cgcccctgga ggacttctgc actttggcca aggaaagtgg tatcccggcg aaccgttgcc
     1921 ccgctgggcc tttagcctct attggcggca ggatggaaaa ccgatctggc aggaagacca
     1981 actctttgcc gaggaaaatc aggcctcaaa catttccgcc gatgatgcca accacttcat
     2041 tcagggaatc gctaaacggg tagaggtggg caccaaaacc attctccccg tctatgaaga
     2101 tccttggcat ttcatcggtc aggaacgaaa gctgccggaa aatctcgaac cggccaccaa
     2161 taatctcgac gatcccatgg ctcgtgcccg gctcgcgaag gtgtatgaac gcggtctggg
     2221 caaacccgca ggctatgtcc tgccattgca acgctggaat gccaaggacg gaccacgtcg
     2281 ctgggtc
//
LOCUS       DATIAL010000316         3317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_703878, whole genome shotgun sequence.
ACCESSION   DATIAL010000316 DATIAL010000000
VERSION     DATIAL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703878"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VLA60_09855"
     CDS             <1..337
                     /locus_tag="VLA60_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09709.1"
                     /translation="SAMPSGNAQIHGQAYQTLPSAVLLTPDLPDRIMEFYKRFLGPGW
                     FQAEDLNMIYFYRLPQPVATGSVLTKQLMSRPGSIPHIAIDMELGPCDQQIGRGARTR
                     ITVVAPPRS"
     gene            complement(377..1090)
                     /locus_tag="VLA60_09860"
     CDS             complement(377..1090)
                     /locus_tag="VLA60_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09710.1"
                     /translation="MPAFLIGWTHGEPPPPGYVAAWFDQAYGGPLRIRFLSSTGHHHF
                     EAGHTNWVAEVNLAPSMEIVEQWRGRLQWEHTRLALLFFQANAHLDRRDDVLHVARMA
                     RGVTLLTDGTAYDVGTGNYHNPSDWQDRDLVIFHLPDHVQVEQREDVQRARIWFHTRG
                     LTKFGLDDIETFQPIGLSGRGVEETVRRVAGRLIEQGKNPKVGEHILLDGSPAVTVVR
                     HRTDPMYGIPLAFREFRLE"
     gene            complement(1218..1613)
                     /locus_tag="VLA60_09865"
     CDS             complement(1218..1613)
                     /locus_tag="VLA60_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09711.1"
                     /translation="MKMVKAPLAFIQALHKRPIACPTPQCSGPVAVEELTRSTDRVKS
                     FELRCEQCDWHDTMMGDLQVDPPWDEGSLMEITEEHLLHLEPVCPYDQAPVDFHSLPN
                     PRRRARYRISCFFCGRQEELDWPPEEAKR"
     gene            complement(1610..2386)
                     /gene="pgl"
                     /locus_tag="VLA60_09870"
     CDS             complement(1610..2386)
                     /gene="pgl"
                     /locus_tag="VLA60_09870"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HSF09712.1"
                     /translation="MRTIIITRTSEELFETAAKEVVKVIKDCRQQGRECCIALAGGST
                     PRGLYRLLAGEPYRSQIPWEHLRLFWGDERPVPPDHPESNFRMAEEALLSHVPIPAQQ
                     VFRIEGELPAEEAAARYEAVLREQFRLTPGQIPEFDLVLLGMGPDGHTASLFPGTSAL
                     SELHKLVTAPWVEKFHTHRVTLTLPVLNAAKYVMFLVSGQDKATALQAVLEGTSDPAR
                     YPATCINPTSGHLTWLINHDAAVLLRKDTMTSMGDKGGIQ"
     gene            complement(2506..2817)
                     /locus_tag="VLA60_09875"
     CDS             complement(2506..2817)
                     /locus_tag="VLA60_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09713.1"
                     /translation="MIKHYLLMGLFAMLCIGCGGNPYLEASLDPAKFEGKDKVWFEEN
                     WGKPSGKSARFFGGESWVYSRIAGGESRFPFFNFSPHQCQIILDFDKEGKLDDYEYQD
                     C"
     gene            3097..>3317
                     /locus_tag="VLA60_09880"
     CDS             3097..>3317
                     /locus_tag="VLA60_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HSF09714.1"
                     /translation="MKLYLDTGSVKEIREAANYGSVDGITTNPSLVAKEGRDFKDLLL
                     EICEIVDGPISAEVVSIEAAAMVKEGRELA"
BASE COUNT          831 a          915 c          782 g          789 t
ORIGIN      
        1 cagcgccatg cccagcggca acgcgcaaat tcacggacag gcatatcaga cgctcccgtc
       61 agccgtgctc ctcacgcccg atctgccaga caggataatg gagttttaca agagattttt
      121 agggccgggc tggtttcaag ctgaagacct caacatgatc tatttctatc gcctgccaca
      181 acctgtggca acgggttccg tcctcaccaa gcagctcatg agcaggccgg ggtctattcc
      241 ccatattgct attgatatgg aacttggacc ttgcgatcaa caaatcggac gaggagcacg
      301 cactcggatc accgtggttg ctccaccccg ctcataacct tcgaattcaa tttttcctgt
      361 ctcgggttga agtcccttat tccaacctga actcccggaa tgccagggga attccataca
      421 tgggatcggt tcgatggcgc accaccgtca cggcagggct accatcaagg agaatgtgtt
      481 ccccgacctt cgggtttttc ccctgttcga ttaatcgacc cgccaccctg cgcacagttt
      541 cttccacccc tcgacccgat agtccaatgg gttgaaacgt ctcgatgtca tccaatccga
      601 atttcgtcaa ccccctcgta tggaaccata ttctcgcccg ctgcacgtct tcccgttgct
      661 ccacttgaac atgatcgggg aggtgaaata tcaccaaatc ccggtcctgc caatccgaag
      721 gattatgata attgccggtc cccacatcat aggccgttcc gtccgtcagc agagtgactc
      781 ctctcgccat tctcgccaca tgaagcacgt catcccgccg atcaagatgc gcattggcct
      841 gaaaaaatag gagggccaat cgggtgtgtt cccactgtaa ccgtcctctc cactgctcaa
      901 caatttccat ggaaggtgcc aaattgactt cggcgaccca attagtgtgt cctgcttcaa
      961 aatgatggtg cccggtcgat gagagaaacc gtatgcggag cggccctccg taagcttgat
     1021 cgaaccaggc ggcgacataa cctggcggag gcggctcgcc atgcgtccat ccaatcagaa
     1081 aggcaggcat agtctgggtg gcggatttct ttttttttat ttttttcatg gattcgagag
     1141 aaaaagagcc ggagacatcg aaaggggcat tagacaagaa ttaggaaaag ggaatgccgg
     1201 aagacgccga cacatgctca tcgcttcgct tcttcgggag gccaatccaa ttcttcctgc
     1261 ctcccgcaaa aaaaacacga gatgcgatag cgggcacgcc gacgcggatt gggcagggaa
     1321 tgaaaatcga ccggcgcctg atcatacggg cacaccggtt ctaaatgcag aagatgctcc
     1381 tcggtgattt ccatcaaaga gccttcgtcc catgggggat ctacttgcag atcgcccatc
     1441 atcgtgtcgt gccagtcgca ttgttcgcac ctcagttcaa atgatttgac ccgatcggtc
     1501 gagcgggtca gctcttccac ggccaccggg cccgaacact gcggagtggg acaggcaatc
     1561 ggccgtttgt gcaaggcttg aataaatgcc agaggcgcct tcaccatttt cattggatcc
     1621 ctcctttatc acccattgat gtcatcgtgt cctttcgcaa caggacggct gcatcatgat
     1681 tgatcaacca cgtcagatga cccgacgtcg gattgataca tgtagccgga tatcgagcgg
     1741 ggtctgaagt cccttctaac acggcctgaa gagcggtcgc cttatcctgg ccacttacca
     1801 ggaacataac atatttcgca gcattgagaa ccggtagagt cagtgtcaca cgatgggtat
     1861 gaaatttctc gacccatgga gccgtgacca atttgtgcaa ttccgatagg gcggatgttc
     1921 ccggaaacaa tgatgccgtg tggccatccg gccccatgcc caggaggacc agatcaaatt
     1981 cgggaatctg tcccggtgtc aacctgaatt gttcccgcaa gacggcttca tatcgggcag
     2041 cagcttcttc agccggtaat tccccttcaa tccggaatac ctgctgagca ggaatgggga
     2101 catgagacaa taatgcctct tcagccatcc ggaaattact ctcaggatga tcgggaggaa
     2161 ccgggcgttc atctccccaa aacaatcgaa gatgttccca gggaatctga gaccgatagg
     2221 gttcaccagc caataaccgg tacaaacctc gaggggtaga cccgcctgcc agagctatac
     2281 aacactcgcg accctgttgc cggcaatctt ttatcacctt caccacttcc ttagcggcgg
     2341 tctcaaacag ttcttcactg gttctggtga taatgatggt ccgcaccaat tcaaagtcct
     2401 acgcactgct ggcgggtggg ctgactgaag gacggaatta cctctggaat tggagattga
     2461 caatcatgtc tcttcactcc gcatgtcaaa ctcgaagatg actccttagc aatcctggta
     2521 ttcgtaatca tcgagtttgc cttctttatc aaaatccaaa ataatctgac actgatgcgg
     2581 agaaaaatta aaaaacggaa accggctttc tcctccggca atacgcgaat acacccagct
     2641 ttctcctcca aagaagcggg ctgacttccc gctcggtttt ccccaatttt cttcgaacca
     2701 gactttgtct tttccttcaa atttggccgg atccagtgat gcctctaaat atggatttcc
     2761 tccacagccg atacataaca ttgcaaacaa ccccatcaac agatagtgtt tgatcacata
     2821 ttctccttcc ctaccctgtc ggaaaatgag gaattacctt tatgctctgt aaaaattcta
     2881 gccctcagtc ctttaccagt caaactactc cagcctcttc tccttcatcg tagaacggtg
     2941 ggatctgatg aattcgggca gactgcaggg gacaatcccc tacaggtaaa aacctcccct
     3001 cgtaaaatgc tcttgaagta tttgtgcaag accaagcggt ggattacaat gaaacatgag
     3061 accaacaagc caccttacat ataaggggat tcaattatga agctctatct cgataccggc
     3121 agtgtaaaag agattcgcga agcagccaat tatggctctg ttgatggtat cacgaccaat
     3181 ccttctcttg tggcaaagga agggcgtgat tttaaagatc tgcttctgga aatttgtgaa
     3241 attgtggatg ggcccatcag tgcggaagtt gtgagtatcg aagccgccgc catggtgaaa
     3301 gaaggaagag aattagc
//
LOCUS       DATIAL010000317         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1349279, whole genome shotgun sequence.
ACCESSION   DATIAL010000317 DATIAL010000000
VERSION     DATIAL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349279"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VLA60_09885"
     CDS             <1..309
                     /locus_tag="VLA60_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF09715.1"
                     /translation="SDVEVVGEAGDGMEAIRLAESLQPDVVVMDINMPNMNGIEATRQ
                     IRQNHPHIHVIGLSVRQDKETEHAMREAGAAGYLSKETAGQDLYRMMASIVRLHQNFT
                     "
     gene            444..1946
                     /locus_tag="VLA60_09890"
     CDS             444..1946
                     /locus_tag="VLA60_09890"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF09716.1"
                     /translation="MPVYTAILALVLGLWAGLGMAAEDVENTAEVTGEEVEEVHDIGI
                     AVAIKNRLSRVDRKSFSSIEVSVKDGIVTLTGTADSLWIQQRATELSQAIRGVRGVID
                     RISVGPVGTTNDAALKKHLEYLFLEDSVVERDDIQVDVVEGVLTLKGRVHSRQEKHAA
                     LNLGKMIKGIREVRDFLKLENVRERPDSILKEEITHRLAFDVWVVNPSLLHVQVEKGR
                     AILTGQVGSVYEKSRIAELAWVEGVRSVDVSGIVVNWVSPDPMMRTQRPTLTDRAISN
                     AIQKVLAYDRRVAPFGISVTVKNGTVTLKGTVPFLSIRREAEENANNTVGVQSVNNLI
                     TVQSKATSKDADIHARLFAAFSRDPVLEPFTLGGAVKKGAVLLTGTVDSIYERNHAEN
                     VASRIRGVTSLTNHISFSNPEIEKTDWEIQLDFENQVWWSPFLSSQDIVATVEDGKAT
                     LSGSVQHMHQRLMAEQQAFEAGASLVINRLRVGKSLPSSELSSQEIYARR"
     gene            2187..>2582
                     /locus_tag="VLA60_09895"
     CDS             2187..>2582
                     /locus_tag="VLA60_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09717.1"
                     /translation="MKCTISTPGQHVVLTLTVDLSINTPNLSRTDVLLDRVVSSPISI
                     LILDLRTLSCVNAIGTWTIFQIVFKAREQGKGVFLYNVQPSIQPYLKEAGIFELSTTI
                     DTQEELGNVLEGNYRLPRSCANGENLKTSL"
BASE COUNT          649 a          602 c          701 g          630 t
ORIGIN      
        1 tccgacgtcg aggttgtcgg agaagccggg gatgggatgg aggccatccg tctggcagaa
       61 tctctgcaac cggatgtagt ggtcatggat attaatatgc caaacatgaa tgggattgag
      121 gccaccaggc agattcgtca gaaccacccc catattcatg tgatcggatt atcggttcgt
      181 caggataagg aaacggaaca cgccatgcgt gaagccggtg cggctggata tttgtccaag
      241 gaaactgccg gacaggacct gtatcgcatg atggccagta ttgtccggct gcaccaaaac
      301 ttcacctaag cctcaacgca gattctgaat cccggatatt ttctgttcat gcccgccaaa
      361 tttgtcaact gacatccccg gtgtttttcc tcattgtaac caggaccgtt cctgttcatg
      421 tttcagctaa tgggggcacg catttgccgg tttacactgc catacttgct ttggttttag
      481 gtctatgggc cggccttgga atggccgctg aggacgtgga aaataccgcc gaggttaccg
      541 gagaagaggt tgaagaggtt catgatatcg ggatagccgt ggccattaaa aaccggttgt
      601 cccgggtcga tcgcaaatcc ttttcctcaa ttgaagtgtc ggtgaaggac ggcatagtca
      661 cgctgaccgg gacagccgac agtctttgga ttcaacagcg agccacggaa ctttcccagg
      721 ctattcgtgg cgttcgaggg gtcatcgatc gcatttcagt gggaccggtc ggcacaacca
      781 acgatgctgc cctgaaaaaa catcttgaat acctcttcct cgaagattct gtggtggaac
      841 gcgacgacat tcaagttgat gtggtcgagg gggtcctcac cctgaagggt cgggtgcact
      901 cccggcaaga aaaacacgcg gcgctcaatt taggcaaaat gatcaaaggc attcgtgagg
      961 tccgggattt tctgaagctg gagaatgttc gagaacggcc ggattcgatc ctgaaggagg
     1021 aaatcaccca tcgcctggca tttgatgtct gggtggtcaa cccatctctc ttacacgtcc
     1081 aggtggaaaa aggtcgcgcg attctgacag gacaggtggg aagcgtgtat gaaaaaagtc
     1141 ggattgccga attggcatgg gtcgagggtg tccggtccgt ggacgtgagc ggtatcgtcg
     1201 ttaattgggt gtcgccggat ccgatgatga ggactcagcg tcccacgttg acggacaggg
     1261 caatttccaa tgccattcaa aaagtgctgg cgtatgaccg ccgggtcgcg ccattcggaa
     1321 tcagtgtgac ggtaaaaaat gggacggtga ccctgaaagg aactgtaccc tttctctcca
     1381 tcaggcgtga ggctgaggaa aacgcgaata acacggtcgg ggtccaatcc gtgaacaatc
     1441 tcatcaccgt ccagtctaaa gcgacttcaa aagatgccga tattcatgcc cggttattcg
     1501 cagcgttttc acgagatcct gtcttggagc ccttcaccct tgggggggcg gtcaaaaagg
     1561 gagccgtcct gctgacgggg accgtcgatt cgatttatga acgcaaccat gccgaaaatg
     1621 tggcttcccg gatccgaggt gtgacaagcc tgaccaatca catcagtttt tcgaatcccg
     1681 aaatcgaaaa aacggattgg gaaattcaac tggatttcga aaaccaggtg tggtggagcc
     1741 cattcttatc gagccaggat atagtggcga cggtggagga tggtaaggcg accttgtccg
     1801 ggtcagttca gcatatgcac caacggctta tggcggaaca gcaggcgttt gaggcaggag
     1861 cctcgctggt gatcaatcgg ttgcgagtcg gaaaatctct cccttcttct gagttgtcaa
     1921 gccaagaaat ttatgcccga cgttgaaagt cgggatggtc caaatcggaa taaatcaaat
     1981 ccaatttcac tgtaatcccc atcgtttcaa gtcctgcatg aaatcctctt ttgagtgccc
     2041 gtggaaggca tgcaatgctt ccccatagtc cgcttaagta tttaacctca agggtaatag
     2101 agagtagcgc gaataaaggg gatgcgtaaa atcaaaagaa tttttttata cctttttgtt
     2161 cttatcaccc aaagggaggt ccttgtatga aatgcactat tagcacccct ggtcaacatg
     2221 tggtcctgac acttaccgtg gatctcagta ttaatacccc caatttaagt cgaaccgatg
     2281 tattattgga ccgtgtggtt tcgtcaccga tttccatact cattttagat ttacggacct
     2341 tgtcatgtgt aaatgctatt gggacctgga ccatcttcca gattgtgttc aaagccaggg
     2401 agcaggggaa gggtgtgttc ctttataacg tgcaaccttc tatacaaccc tatttgaaag
     2461 aggcaggaat atttgaactg tccaccacaa ttgatacgca ggaggaactc ggaaatgtgt
     2521 tggaagggaa ttaccggctt ccgaggtcat gtgcgaatgg ggaaaacttg aagacctcat
     2581 ta
//
LOCUS       DATIAL010000318        12005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_704067, whole genome shotgun sequence.
ACCESSION   DATIAL010000318 DATIAL010000000
VERSION     DATIAL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12005
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704067"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1122
                     /gene="ttcA"
                     /locus_tag="VLA60_09900"
     CDS             301..1122
                     /gene="ttcA"
                     /locus_tag="VLA60_09900"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HSF09718.1"
                     /translation="MLQDIQPIQPLSHLTGSSEKLHTRLCRMVGQAVADFNMIEGGDK
                     VMVCLSGGKDSYAMLDVLMSMQSRAPVSYELIAVNLDQKQPGFPEHVLPDYLTRLGVP
                     FHIEERDTYSVVKRLIPEGETTCSLCSRLRRGILYGLAERLGATKIALGHHRNDMMET
                     LLLNMLFNGTLKSMPPKLRSDDGQHVVIRPLAYVKEAELARYAEMRNFPIIPCDLCGS
                     QENLKRKEVKALLQEWDTRYPGSGDSVFAALSKVIPSHLLDRQLFDFETFRELES"
     gene            1188..1712
                     /locus_tag="VLA60_09905"
     CDS             1188..1712
                     /locus_tag="VLA60_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HSF09719.1"
                     /translation="MPSPLLCVRFLPGIIMVYLQVFLLIMAANAAPIFGRLLMKGRWS
                     APLDGGATFFDGQPWLGPSKTYRGVAFSLVGTVLAADVLAMPWDIGLLTGGLAMTGDC
                     LSSFIKRRLGYASGGMALGLDQIPESLFPLLGLWGPLSLSAIGIMATASAFMVFELFI
                     SPILYRFRIRKNPH"
     gene            complement(1735..1938)
                     /locus_tag="VLA60_09910"
     CDS             complement(1735..1938)
                     /locus_tag="VLA60_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09720.1"
                     /translation="MPCSPQAFSTHQAKDRVLVWPLQDGGLITYEQPDGRLIHTLNTP
                     DGFSRKLGQLGITLTTTKNPDKE"
     gene            2275..2658
                     /locus_tag="VLA60_09915"
     CDS             2275..2658
                     /locus_tag="VLA60_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09721.1"
                     /translation="MSIGFAVFAVLMVPVFLYSMGPDGPAKVGDVVFATDRHRVRIMV
                     SNDEEIGRYSAMCVLEPRVQLVVQQMGPPSESAMIAEPVGLEKIDPPFCLPGKPVMVY
                     PHQVTLQPDLWGGLRDTLLQFSSRR"
     gene            complement(2694..3260)
                     /gene="msrA"
                     /locus_tag="VLA60_09920"
     CDS             complement(2694..3260)
                     /gene="msrA"
                     /locus_tag="VLA60_09920"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HSF09722.1"
                     /translation="MSDSAIASSTGKELATLGGGCFWCLEAVFDQVKGVESVESGYMG
                     GSRPNPTYEAVCSGNTGHAEVIQITFNPGIITYRELLEIFFGIHDPTTLNRQGNDAGT
                     QYRSAIFTHSSAQDATAHEVIRTLTEAKLFNDPIVTQVEPASPFYMAEAYHQEYFVRN
                     PSQPYCAYLINPKVAKFRQRFSDKLKSS"
     gene            3485..4381
                     /locus_tag="VLA60_09925"
     CDS             3485..4381
                     /locus_tag="VLA60_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HSF09723.1"
                     /translation="MRVVWATDIHLNFPGFKGRDVFFSSIRAQQPDVVFVTGDIAEAP
                     SLTHLLHEMRQAIQVPLYFVLGNHDFYYGSISRIRNDLQRWCQSQPGLTYLSTSGLVE
                     LTPTTALVGHDGWGDGRYGNYHLSPVRLSDQELIADFQDLGREAVLRKLRALGDEAAC
                     YLRDRLDEALSSYHRVICLTHVPPFKEACWYQGKMGNDDWLPYFACQAVGEVLLYASR
                     ERPDCQITVLCGHTHHAGTVHLRPNLRVLTGSAEYGAPCIQESFDLEELFPQSMLVEG
                     GEGEGGQSVETDVAGRPSGPAK"
     gene            complement(4374..4808)
                     /locus_tag="VLA60_09930"
     CDS             complement(4374..4808)
                     /locus_tag="VLA60_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HSF09724.1"
                     /translation="MKQGSLVQSHEWGQHEKVIVFDGVCNFCNAFVNFVLERDSRGLF
                     KFATLQSQPAQDILTHLHLSTQDYETFLLLEHGKVFTKSTAALKIFRSLPGAWRWLYA
                     FIIIPRPLRDMVYSFIARHRYQWMGKSDTCRVPTPKERARFI"
     gene            complement(4926..5888)
                     /locus_tag="VLA60_09935"
     CDS             complement(4926..5888)
                     /locus_tag="VLA60_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09725.1"
                     /translation="MASCKSINRIWNQFFFTGFSGESLGLLRMYIGIGLLFFHTYQFA
                     TVLTLNPIGAMYYFVEPIWYFKLLGIQYHIPALSFVMYIILMYATVSMIAGKNTRASI
                     LVIILCIFYLKGVRDSFSGDVHHRYIIPMQILFLFLLSKCGEVHSRDARQGHIHEGIQ
                     EWEASWPIKTMQLYVALFYFWSVIAKVRVSGWVWFAGEGRIQEVLIKRSVRWGVTDQG
                     EVVKNGLSFELAQHPDLIQFFGILVLVFELGFPLLLLIKSVKLRLAFILGVTAFHIST
                     FVLMDVNFLLIPFVYLIFFDLVPIHQWIKSRAWPLFRRGPSIAG"
     gene            complement(5875..6471)
                     /locus_tag="VLA60_09940"
     CDS             complement(5875..6471)
                     /locus_tag="VLA60_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09726.1"
                     /translation="MSQRTITLILIVYLTIIWAAVIFRIDYFPLTWVPMYSVYEPSET
                     ISTSIKDKAAIKKGFRVTHRDGSISYVSYKDLNIPKPNFGRLYSAIIFGHDPPKHKQG
                     NTSLGSINRWIRGLDDNERNFSVDWEWRMFWTINKTLRFEPGDQKFITRIESSHQKRV
                     YKKADLLKGDTGKAEIQTNTVVIDWQDQWIERWNHGLL"
     gene            7074..7679
                     /locus_tag="VLA60_09945"
     CDS             7074..7679
                     /locus_tag="VLA60_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09727.1"
                     /translation="MTVAVFIDRLEEFRQGVLRYREMAEPNQGLPRNGSVGMDSSPSK
                     ALRDILNQQFTSIEESVGTFSRGRYRIHLDSRGREDIYSHALSNECQAEHLDLILNDL
                     ELMLEELQKRPPHSLLVSQEEWPDNSGTNPEPSFGSLGHLGSSPSPASSDTLHAALNT
                     VASVIHQRVDRSQDQEELLAHVQAILDHPKITHLLSFSPSR"
     gene            7983..9287
                     /locus_tag="VLA60_09950"
     CDS             7983..9287
                     /locus_tag="VLA60_09950"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HSF09728.1"
                     /translation="MAKSSEQFLHWSISRVVPVDRDELPALIWSFVYFFCVLAAYYIL
                     RPVRDEMGVLSGAHNLPWLFSVVFITMLAVIPMFGWVAGHFRIRRLLPTVYVFFILNL
                     LAFFVALQIGVGLQAFGPIFFVWLSVFNLFVVSVFWSFMADVFRTDAARRLFGCISAG
                     GSLGAMTGPFITAMAVKGVGVSGLLLVSAIFLLIAVGCIMALLKWYHGHHGIDLGNRH
                     LSSIRNSEATPPSLWVGVVCIVRSAYLRGIALYLMCYSILSTFLYSQQTILIPQVMPS
                     SEDRMQLFASVDLGINVFAFTLQFFATGWLLTRFGVVIMLAVMPLVSLIGFGVFGLMT
                     VLPVLIGFGVLRRAGEFSITKPTRETLFTVVSREEKYQAKNVIDTVVHRGADMTGTGI
                     VSVFHAWGMTLSHMAFAALPIAGLWLATGVWLGRRHEAIRRR"
     gene            9392..9886
                     /locus_tag="VLA60_09955"
     CDS             9392..9886
                     /locus_tag="VLA60_09955"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HSF09729.1"
                     /translation="MSDALFTYGTLQFAEVMEAVTGMALPWVEAEAPGFAQFRLRDRI
                     YPGMVAREGALTRGRVYTSMSHQTWELLDRFEDPVYRRELLEVFRPDGSTMTAHAYVL
                     PVAQQHLLSSDLWQMDWFSHVHLDGYVSRCRAFYEAITSQGLSESCKQTLWKVTDSSQ
                     NSQA"
     gene            9883..10296
                     /locus_tag="VLA60_09960"
     CDS             9883..10296
                     /locus_tag="VLA60_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09730.1"
                     /translation="MMKSFNRPFLLVMLVTLLVFGGALVYFTMEYLSQVTNPDITLTE
                     STGHQISMLLLVVTMLAGMPAVGMGAYVMYLGSRIRLTQQWPPAGMGFGAETPVMLGD
                     RATLVGWGVTGLGFVLVVCGLILPVVGWEFGNMVQ"
     gene            10348..>12005
                     /locus_tag="VLA60_09965"
     CDS             10348..>12005
                     /locus_tag="VLA60_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HSF09731.1"
                     /translation="MTGWDWAILAVFILYALQAGFRERAVASQNLEEYFLAGRSLSGW
                     KAGLSMAATQFAADTPLLVTGLVATAGIFALWRLWIFALAFLLMGFLLAPSWRRVGVL
                     TDAELTEVRYGHRAASALRGIKAIYFGTIVNCTVLAMVLLAATRLAEPFLLWDQWLPA
                     GIFDVVVHIVKWGGVPFTVGGLEADNVWVRSANNLLSIGAIVSVTVLYSTTGGLRSVV
                     ATDLVQFGIGIVASGAFAWVVVDHVGGLASLTQHIQQQFSVAVGYPLTGNEILAFTPF
                     GARDATMMVLLVYGFQWLLQMNADGTGYLAQRSMACRSDHDARVAAVVFTVAQVVLRS
                     LIWLPLALGLLVIFPPPPEMIGPQFIADREFTFVQGIHELLPPGIKGLMVVGMLAALA
                     STVDTHLNWGASYWTNDIYRRFICEGWLKREPSQRALVWVARASNLLILLIALCILPW
                     LSSIQTAWQISLLLGAGMGVVLVLRWIWWRITAWGELACIAASVLMAPLLLWALPGQQ
                     EEMRLLIMGLGAGAIGVAASWFTGPEDLDRLETFYRRARPPGFWG"
BASE COUNT         2575 a         2735 c         3395 g         3300 t
ORIGIN      
        1 tgcctcttgg ggaaccggcc tcccgtccaa gatgggaaaa aggaaacgaa agatctgagg
       61 atgcctggtc cttcttcagc cggtcccaat tccttgtctt gaccccatct ggtgggtgac
      121 acggtcttca cccggtcttt cctgccattc cattgggttg atcccgcctc tgactgtcta
      181 tatcttttac tgtttcggag ggtagaatgc ggtaatcgtg caggttgcag cggctgttgt
      241 acgtccctgt gaattataac gaataaatct ttttcagaaa tgaggagtag agacgaagct
      301 atgttgcagg atattcaacc catccaaccc ttgtcccatc tgaccggatc cagcgagaag
      361 cttcataccc gtttgtgccg aatggtggga caggccgttg ccgatttcaa catgattgaa
      421 ggaggcgata aggtcatggt gtgcctttcc ggggggaagg atagttatgc gatgttggat
      481 gtgttgatgt ccatgcagtc tcgtgctccg gtgtcctatg aactgattgc ggtgaatttg
      541 gatcagaaac aaccgggatt tcctgaacat gtgctgccgg actacctgac gaggcttggt
      601 gtgccgtttc atattgaaga acgcgatacc tattcggtgg tgaaacggct gattcccgaa
      661 ggcgagacga cctgctcact ctgttcacgg ttgcgtcgtg gcatcctgta cgggttggcg
      721 gaacggttgg gcgcgaccaa gattgcactt ggccatcatc gcaacgatat gatggagacc
      781 ttgttgctga acatgttatt caacggaacc ctcaaaagca tgccgccgaa actgagatct
      841 gatgacggac aacatgtggt gatccgccca ttggcatatg tgaaggaagc cgaactggcc
      901 cgctatgcgg aaatgagaaa ctttcccatc attccctgcg acctgtgcgg ttcacaggaa
      961 aatttgaaac gcaaagaagt gaaggccctg ctccaggaat gggatacccg gtatcccggc
     1021 tcgggtgaca gtgtgtttgc ggccttgtcc aaggtcatac ccagtcatct cctcgatcgt
     1081 caactatttg attttgagac ttttcgcgag cttgaatcgt agattggcct gacgttggtt
     1141 cccgtgcgaa tgatgaccga cctgccagat cccattcgat tgtgtgcatg ccctcgcccc
     1201 tcctgtgtgt gcggtttttg ccgggcatca taatggtcta tctccaggtt tttttgttaa
     1261 ttatggcggc caatgccgcc ccgattttcg ggcggctgct tatgaaaggg cgttggagtg
     1321 ctcctcttga cgggggagcg accttcttcg atggacagcc gtggttggga ccctcgaaaa
     1381 cctatcgagg cgtggctttc tcgttggtag gcacggttct ggcggcggat gtcttggcta
     1441 tgccctggga catagggctg ttgacgggag ggctagcgat gaccggcgac tgcctctcta
     1501 gtttcatcaa gcgacggttg ggctatgcct caggcgggat ggccttgggc ttggaccaaa
     1561 ttccggaaag cctttttccc ctgttggggc tatggggacc cctgtcgtta tcggctattg
     1621 gcataatggc cactgcaagt gcgtttatgg tgttcgagct gttcatttct cctattttat
     1681 acagattccg cattcgtaaa aatccgcatt aattctttgt ttccgttact gaatttattc
     1741 cttgtcagga tttttggtcg tggtgagggt gatccccaat tggccaagtt tccggctaaa
     1801 tccgtcaggc gtgttgagtg tatggattaa gcggccatcc ggctgttcgt aggtaataag
     1861 tccaccatct tgtagcggcc agacgagtac ccgatctttg gcctgatgtg tggaaaaagc
     1921 ctgaggggaa catggcatgc cgagtagcat ttcgaaagaa acgggaggct ccaacctggg
     1981 gagaacgagc cgtcccggac aatttcgaat gggccgccag ggccagcgag ttcgtaattc
     2041 ctcaaaggtc atcggagcgc tttcttgacg tgctcattcc actccgagat ggatgcgttt
     2101 gggaatctta gtggaagaga ggatggctcc ggtgtatgct tcatgccatg gtccatgaaa
     2161 taatgcccgg atgtgtcagg aacagcgaag ccaacatact gagatcctgg cgtggggatg
     2221 aatgtggatc cctcatctga agaagcatct tcgggcaatt ccaggacacc cctcatgtcg
     2281 ataggattcg cggttttcgc cgttctcatg gtaccggtat ttctatattc tatgggcccg
     2341 gatgggcctg ccaaagttgg tgacgtggtg tttgccacgg atcgtcaccg tgtacggata
     2401 atggtttcca atgatgagga gattggaaga tattccgcga tgtgtgttct tgagccacgg
     2461 gtacaattag tggtgcagca aatgggccct ccctcagaga gtgcgatgat tgcggaaccg
     2521 gttggccttg aaaagattga tcctcccttt tgccttccgg ggaagccggt tatggtgtat
     2581 ccccatcagg tgaccttaca gccggacctg tggggtggcc tgcgggacac gcttttgcag
     2641 ttttcctctc gtcgctgagc aggacgaccc cgaagccgtt tatctccagg cactcaggag
     2701 gatttgagct tgtccgaaaa gcgttgccgg aacttcgcca ctttgggatt aatgaggtag
     2761 gcgcagtaag gttgtgacgg gttccgcaca aaatattcct ggtggtaggc ttcggccatg
     2821 taaaaggggg acgcgggttc cacctgagtc acaatgggat cgttgaacaa cttggcttcc
     2881 gtcagcgtgc gaatgacctc gtgcgcggtc gcgtcttgtg ctgatgaatg ggtgaaaatg
     2941 gcggagcgat attgggtgcc cgcatcattc ccctgtcggt tcagggtggt tgggtcatgg
     3001 atgccgaaaa agatttccag gagttcccgg taggtgatga ttccgggatt aaaggttatt
     3061 tgtatcacct cggcatgtcc cgtgtttccg ctgcagacgg cctcataagt tggattgggg
     3121 cgactaccgc ccatatagcc ggattctacg gactcgacgc ccttcacctg atcgaatacg
     3181 gcttcgagac accagaagca gccgccaccc aatgtggcga gttccttccc tgttgatgaa
     3241 gctatggccg agtcagacat gcgttactcc ttgctcatga aaatgactaa agctgtgagg
     3301 tcaactgtaa gagttcagag tttataccac gccctgcttt cttgggccac tgcttgttgc
     3361 acgggtggag aggcgagagt gacgtttccc ctgaaattct ccgctttact ttttgtccgg
     3421 cctctttata ggcttggacg atggtctttc agactgttga gtagtgatga gaggagcaag
     3481 tcatatgcgg gtagtgtggg cgaccgacat ccatttgaat tttccgggtt ttaagggacg
     3541 ggacgttttt ttttcgtcca ttcgtgctca acagccggac gtcgtctttg tgaccggaga
     3601 cattgctgag gctccatcgc tgacccatct cctccatgaa atgcgtcaag ccattcaagt
     3661 gccgttatat ttcgtcctgg ggaatcatga tttttactat ggttcgattt cccgaattcg
     3721 aaatgatttg caacgctggt gtcagagtca gccaggactc acctatcttt cgacatcggg
     3781 gttggtcgaa ttgactccga cgacggcgtt ggtcggacat gacggatggg gagacgggcg
     3841 ctatgggaac tatcatctgt ccccggttag actcagtgat caggagttga tcgcggattt
     3901 tcaggattta ggtcgggagg cggtgttacg caaacttcgt gcgttgggag atgaggcggc
     3961 ttgctatttg cgggatcgat tggatgaggc cttgtcatca tatcatcgcg tcatttgcct
     4021 gacgcatgtc ccgccattta aagaagcctg ctggtaccag gggaaaatgg gaaacgatga
     4081 ttggcttcca tattttgcct gccaggcagt tggtgaggta ttactttacg catcacggga
     4141 acgaccggat tgtcaaatca ccgttttgtg tggccatacc catcatgcgg ggaccgttca
     4201 cctgcgaccc aatctgcggg ttcttacggg ttctgccgaa tatggtgctc cgtgtattca
     4261 agagagcttt gaccttgaag aactgtttcc ccaatcaatg ttggtagaag gcggtgaggg
     4321 tgaaggaggg caatcggtag aaactgatgt cgccggaagg ccatcaggtc ccgctaaata
     4381 aatctggccc gttctttcgg agtaggcaca cggcacgtgt ccgattttcc catccactga
     4441 tagcgatgcc gggcgatgaa gctgtacacc atatctcgaa gaggccgggg aatgatgatg
     4501 aaggcataca gccaacgcca agctccagga aggctgcgaa aaattttgag agctgcagtg
     4561 gattttgtaa agacctttcc gtgttccagc agtaaaaagg tttcataatc ctgggtcgaa
     4621 agatgcaggt gagtgagaat gtcctgtgcc ggttgggatt gaagcgttgc gaatttaaat
     4681 aatcctcggg aatcccgctc gaggacaaaa ttgacaaacg cattgcaaaa attacagacc
     4741 ccgtcaaaga caatcacctt ctcgtgctgg ccccattcat gggattgtac tagcgatccc
     4801 tgtttcacgt gcccctctta gttgtcaggt ctttccttaa cgtgtacggg tatatctggc
     4861 atggcgcacc tccttattat aatacctcag aggtggatat gtacgcatgg gtggggctgt
     4921 cgggctcacc ctgctataga gggcccacgc ctgaataatg gccatgcgcg ggatttgatc
     4981 cattgatgaa tgggaacgag gtcaaagaaa ataagataga caaatggaat taataaaaag
     5041 ttaacgtcca tcaagacaaa ggtggaaata tgaaatgcgg taactcccaa aataaatgcg
     5101 agccgcaact tgacggattt gattaagaga agcaggggaa agccgagctc gaaaaccagg
     5161 acgagaattc cgaaaaattg aatgaggtca gggtgctggg ccagctcaaa gctgagccca
     5221 tttttgacga cttctccttg atcggtcaca ccccatcgaa ctgatcgttt gatgaggact
     5281 tcctgaattc tgccttcgcc agcaaaccag acccatcctg agacccggac tttagcaatc
     5341 acactccaga aataaaacag cgccacatac agttgcatgg ttttgatggg ccagctagcc
     5401 tcccattcct gaattccttc gtgaatatga ccttgcctgg catcacgaga atggacttcc
     5461 ccgcacttgg aaagtaaaaa gagaaaaaga atctgcatgg gaatgatata acgatggtgc
     5521 acgtctcccg aaaagctgtc tcgcacgccc ttcaggtaaa aaatacagag aatgatcacg
     5581 aggatcgatg cccgggtgtt tttccctgca atcatcgaga cggttgcata cattagaatg
     5641 atatacatca caaatgagag ggccgggata tgatattgaa tccctaacaa cttgaagtac
     5701 caaatgggct caacaaagta atacatggcc cctatcgggt taagtgtcag gacggtggca
     5761 aattggtacg tgtggaaaaa gaggaggcca atcccaatgt acatgcgtaa caggccaaga
     5821 ctttctcctg aaaatccggt aaagaaaaat tgattccaga ttcgatttat tgatttacag
     5881 gaggccatgg ttccatcttt cgatccattg atcttgccag tcgatcacga ccgtgtttgt
     5941 ttgaatctcg gccttgccgg tatcgccctt taataaatcc gcttttttgt acactcgttt
     6001 ttgatgggag gactcaatgc gggtgatgaa cttttggtcg ccaggttcaa atcgaagcgt
     6061 tttgttgatg gtccaaaaca ttcgccactc ccagtcaaca gagaagttgc gctcattgtc
     6121 atccaggccc cgaatccacc ggttgattga ccctagtgac gtattccctt gtttatgttt
     6181 tggtggatcg tgtccaaata tgatggcact gtatagccgc ccaaagttag gcttgggaat
     6241 attcagatcc ttgtatgaca cataactgat tgatccatcg cggtgggtga cacgaaaccc
     6301 tttcttaatg gcggctttgt ccttgatact tgtcgaaatg gtttcgctgg gctcgtacac
     6361 gctatacatg gggacccagg ttaaggggaa atagtcgatt cgaaatatga ctgccgccca
     6421 aatgatggtc aagtagacaa taagaatgag ggtgatggtc cgctggctca cgcaaacctt
     6481 tcttgggatt tattgagcgg ggtattcatg tgggtgtctc cccctagagg tgaaggtccg
     6541 agtgacggga aagcccatta tcttaagaaa gatgagagaa ctcaatagtg agaaggttac
     6601 aaaaaaatat tgttcgtgtg ataagcgaat ggagagcgaa ggggattgtt gtcgattttc
     6661 catgagaata gaaggaaaga agcgcggact aatccttata tttcccggga tagaacggtt
     6721 gagcagtagg aattttcgcg agtcctcctg tccgggtttc tatagcgctg gagtcgatgg
     6781 gctaattttt agtgtggtct ttaaggctgg gattcgaaat ttaaagattt gggactgtat
     6841 cttggttgac ttgtgccgtt ggcggtttct atgttaaaga ctttgttgtc ccaggaagat
     6901 ggtgcgtcct cagtgatctg ctctaggtgt tcctgtcgca atgttttctc tggattgagt
     6961 agtgcttccc tttaggttgg cagatggttt caatgtgtgg gttggagtgg attttgccct
     7021 cagaaccttc acatgtgcgg tcaaatttca caataggtaa ggagtgtgag tcaatgacgg
     7081 tagctgtgtt cattgatcgt ctcgaagaat ttcgacaggg agttctccgg tatagggaaa
     7141 tggccgaacc gaatcagggc ctgccccgca acggctcagt tgggatggac tcatcaccct
     7201 caaaggcctt acgggatatt cttaatcagc agtttacttc catagaggag tctgtaggga
     7261 cttttagcag aggtcgctac cggattcatt tggattccag gggtcgagag gatatttata
     7321 gccatgcgtt gtctaatgaa tgtcaggcgg agcatcttga ccttattctg aatgatcttg
     7381 agctcatgct ggaggagcta caaaaacggc ctcctcattc tcttctggta tcacaggagg
     7441 aatggccgga taattcggga accaatcctg agccttcctt cggctcgttg ggacacctcg
     7501 gctcttctcc atctcccgca tcatctgaca cgctgcacgc cgcgttgaat acggtggcaa
     7561 gcgtcattca tcaacgagtt gatcgatccc aagaccagga ggagcttttg gctcatgtgc
     7621 aagccatcct ggatcatcct aaaatcaccc acctcctatc cttctcccct tcccgataac
     7681 tggaatcttc ggtttctttc aagtcctggg ggtgcaacct cccgatcatt atgtggtggt
     7741 atcagaaaat cctgaagggt attgtgaaag atcgtttctg agcttttccc ctttatggcg
     7801 atcactgcgg ttcctctcca ttttaagtct catttccgtt ttccttaaaa tttattgatg
     7861 ggtatcaaaa cgcatggttc ggcgatatga ggtgcgatgc ctgaagactc taaggacgac
     7921 attcgtcata atgtacccat caatgcgcct cagttatagt gtccaagacg aattcctata
     7981 gaatggcgaa atcttctgaa cagtttcttc attggtcgat cagccgggtg gtgccggttg
     8041 accgggatga actccctgcc ctcatctggt cgtttgtcta ttttttttgt gtgctggcgg
     8101 cctactacat cctgcgaccg gttcgcgatg aaatgggagt gctctcgggg gctcataatc
     8161 tcccgtggtt gttctcagtt gtgttcatca cgatgctagc cgtgatcccg atgtttggat
     8221 gggtcgcggg gcattttcgg attcgtcgat tattgccaac ggtctatgtg tttttcatcc
     8281 tgaatctttt ggcatttttt gtagctttgc aaattggagt cgggttgcaa gcctttgggc
     8341 cgatcttttt tgtatggttg agtgtgttta atttgttcgt cgtgtcggtg ttttggagtt
     8401 ttatggccga tgtgtttcgc actgatgcgg cccggcggct ctttggttgt atttccgcag
     8461 gaggcagtct tggcgccatg acaggcccgt ttatcaccgc aatggcggta aaaggggtcg
     8521 gggtttccgg gttactctta gtgtcagcga tcttcttatt gatcgcggtg ggatgcatta
     8581 tggcgttgtt gaaatggtat cacggccatc acgggattga tttaggaaac cgacaccttt
     8641 cttccattag gaattctgaa gccactcctc ccagcttatg ggtaggagtg gtgtgcattg
     8701 ttcgctcggc ctatttgaga ggtattgccc tctatctcat gtgttattca attctgtcaa
     8761 cctttctgta ttcgcagcaa accatattaa ttccgcaagt catgccgagt tcggaagatc
     8821 gcatgcaatt gttcgcatcg gtggatcttg ggatcaatgt gttcgcgttc acgctccagt
     8881 ttttcgcgac aggctggctg ttgacgcggt ttggcgtcgt gatcatgttg gctgtcatgc
     8941 ccctggtatc attgattgga tttggggtgt ttggtttgat gaccgtcctg ccggtcttga
     9001 tcgggtttgg tgtcctgcgg cgggcggggg aattttccat cacaaagccg acgcgggaaa
     9061 ccctctttac cgtcgtttcc cgtgaagaaa aatatcaggc aaaaaatgtg attgatacgg
     9121 tcgttcatcg aggtgccgat atgacgggga cagggattgt ctccgtcttt catgcctggg
     9181 gaatgacctt gtcacacatg gcatttgcgg cattgcccat tgcgggtcta tggcttgcca
     9241 ccggtgtgtg gcttgggcga cgacacgaag cgatccggcg acgatgaggc ttgttcccga
     9301 gtgaagattg tcatggagtt taatgtgtcc cctgcgatag aaagccttgc ttaagaccga
     9361 tcatcagagg tcattcgatt ggagtcatga gatgagtgat gccttgttta cctatgggac
     9421 gttgcaattt gccgaagtga tggaagcggt gaccggcatg gcgttgccat gggtggaagc
     9481 ggaagcgccg ggattcgcgc agtttcggtt gagggaccgc atttatccgg gaatggtggc
     9541 ccgggaaggc gctctgacac gggggcgtgt gtatacctca atgagtcatc aaacttggga
     9601 gctcctggac cggtttgaag acccggtcta tcgcagggaa ttgcttgagg tcttccggcc
     9661 ggatggctca acaatgacgg cccatgctta tgtcttgccc gttgcccagc aacatctgct
     9721 ttcttcagat ctgtggcaga tggattggtt tagccatgtt catttagatg gatatgtgag
     9781 tcggtgtcgc gcattttatg aagcgatcac atcccagggc ttatcggaaa gctgcaagca
     9841 aacgttgtgg aaggtaacgg attcttcgca gaactctcag gcatgatgaa gtctttcaat
     9901 cgaccgttcc tgctggtgat gctggtgacg ctgcttgttt ttggcggggc cttggtctat
     9961 ttcaccatgg aatatctctc acaggtcacc aacccggata tcacactgac ggagtccacg
    10021 ggtcaccaaa tcagtatgtt actgttggtc gtgaccatgc tggcaggaat gcctgcagtg
    10081 ggaatgggcg cctatgttat gtaccttggg tcacgaattc gtctcactca gcagtggcct
    10141 ccagcgggga tgggatttgg ggcagagacg ccggtcatgt taggggatcg ggccacgctc
    10201 gtcggatggg gtgtgacggg gcttgggttt gttcttgtgg tatgtggatt aatcttgccg
    10261 gtggttggtt gggaattcgg gaacatggtt caatagactg aatgcatctt ttttgacagc
    10321 acacccaatc atgatgagcc aaaaattatg actggatggg attgggcgat tcttgccgtt
    10381 tttattctct atgccctgca agcggggttt cgagagcgag cggtcgcatc ccaaaatctg
    10441 gaagaatatt ttcttgcagg acgcagtctt tcgggatgga aggccggatt aagcatggcg
    10501 gccacgcagt ttgcggctga cacgccattg cttgtcacag ggttggtggc tacggccggc
    10561 attttcgcgt tgtggcgctt gtggattttt gccttggcct ttcttctgat ggggtttctc
    10621 ctggctccga gctggcggcg ggtaggggtg ctcaccgatg ccgagctgac ggaggtgcga
    10681 tatggccacc gtgcggcgtc ggcgctccgg ggtatcaaag caatttattt cggcaccatc
    10741 gtcaattgca cggtgctcgc catggtgctg ttggcagcca ctcgcctcgc tgaaccattt
    10801 cttctttggg atcaatggct tcctgccggc atttttgatg tggtcgttca catagtgaaa
    10861 tggggagggg tcccttttac cgttggcggg ctggaggcgg acaatgtgtg ggtgcgttcg
    10921 gccaataatc ttttatcgat aggggccatt gtgtctgtga cggtgttgta ttccaccacc
    10981 ggtggactac gaagcgtcgt cgccacggat ctggtgcaat ttggaatcgg gatcgttgcc
    11041 agtggggcat ttgcctgggt ggtggtggat cacgtgggag gcttggcgtc cttgactcaa
    11101 cacattcagc aacagttttc agtggcggtt gggtatccac tgaccggtaa tgagattctg
    11161 gcttttactc ccttcggtgc cagggatgcg accatgatgg tgttgttggt ctatggattt
    11221 caatggctct tacaaatgaa tgcggatggg acaggatatt tggctcaacg gtccatggca
    11281 tgccgtagcg atcacgatgc gcgagtcgct gcggtggtgt ttaccgtggc ccaagttgtg
    11341 cttcggagtt tgatctggtt gcccttggcc ctgggattac ttgtgatatt tccaccccca
    11401 ccggaaatga taggccctca gttcattgcg gatcgcgaat ttacctttgt gcaaggcatt
    11461 catgaactgt taccgccagg aattaaaggg ttgatggtcg tcggaatgtt ggcggccttg
    11521 gcctccacgg tcgatacgca tttgaattgg ggagcgtcct attggactaa tgatatttat
    11581 cgccggttta tttgtgaggg atggctcaag cgcgaacctt cgcaacgtgc actggtctgg
    11641 gttgcacgtg cgagtaatct gctgattctc ttgattgccc tttgcattct tccgtggttg
    11701 tcatcaattc aaacggcctg gcagatcagt ttgttgcttg gagcgggaat gggcgtggtg
    11761 ttggtactgc gatggatctg gtggcgaatt acggcatggg gcgaattagc ctgtattgcg
    11821 gcttctgtgc ttatggcccc actgttactg tgggcccttc cgggacagca agaggaaatg
    11881 cggttgttga tcatgggcct aggagccggt gcgattggtg tcgccgcgtc ctggttcacc
    11941 ggaccggaag atctcgatcg cttggagacc ttttaccggc gtgcacgtcc tccgggattt
    12001 tgggg
//
LOCUS       DATIAL010000319         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_489173, whole genome shotgun sequence.
ACCESSION   DATIAL010000319 DATIAL010000000
VERSION     DATIAL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489173"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2405
                     /locus_tag="VLA60_09970"
     CDS             <1..2405
                     /locus_tag="VLA60_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HSF09732.1"
                     /translation="NRRLDRLEPTATPESEKPSADSERLTGSIETVEILELYQDRAIQ
                     ATKSLLELGQSRDFREHFQIDEVMIEGVEGRRRASFDEDEGWWQRLRIATQKDGSLKF
                     EALTNRARAETYLQPAQRKLIERFLERAMATTQNDLELSSTLFELIIPNRLKEYAPDR
                     RKMVLVLDEKSAAYPWELLHNQYDPNSRPLAVEAGMVRQLAVPQFREKVLHGTALNAL
                     VIGDPTSHEPSGKFAPLPGAAAEAREVAQLIRHQEYRDVVELVEEEATPDAILTALYK
                     QPYRIVHCAAHGVFEFPIEDDPAKQGTADSQKASGTPPRMVTGMVLGEGLFLTPTEIN
                     QMRYVPELVFLNCCHLGQTKGSAEQSNIPFHQLASNLGTQLIRMGVRAVVAAGWAVDD
                     MAAKTFAKKFYEEMFQNRTFGDAVQLAREEIFLRQGGSNTWGAYQCYGDPDFSLHIGA
                     KSMGIQRRMVAPVELRVELYNLVQEAKTAEPKDEARLRRRLQELVAGVGQGWTDSAAM
                     CAALGLAYGEFGLFAEAVRFYDRGRMLQPADATVESLEQLANLKVRWALQRLERQADP
                     TAQKLDSLEKDFPLKELFDDAEHILAGLLTIQQTQERYALKGKLYKGKAMLFSTKAEK
                     RKALLEMKHCYAEGYNIGKDAQRHDVYYPLGNRLAAEIVLSWDQPKGRSTRRGKTKGA
                     DPIAEGLAELSVYAKELIGKGQSFWGMSLSPDQKLLEALYVRRLTANDQKEILNGYLE
                     AKRRGGSAREIDSVIKNIRFFESMVATQAPPNIRQQLSAGLKTLRESLVSDGGANGEP
                     ES"
BASE COUNT          729 a          689 c          812 g          640 t
ORIGIN      
        1 ccaatcggcg gttggaccga ctggaaccga ctgcgacacc tgaatctgaa aagccgtccg
       61 cagattccga gcgtctcacg ggctccattg agacggtcga gatactcgag ctgtatcaag
      121 atcgggccat tcaagccacg aaatcgctcc tggaattggg gcaatcacgt gattttcgtg
      181 aacatttcca aatcgatgaa gtgatgatcg aaggggttga ggggcgccgc cgtgcctcct
      241 ttgatgaaga tgagggctgg tggcaacgat tgcgcattgc cacccaaaag gacggctcct
      301 tgaaattcga agccctcacc aatcgggcgc gggcggaaac ctatttgcag ccggcacaac
      361 gaaaactcat tgaacggttt ttggaacggg ccatggccac cacacagaat gatctggaat
      421 tatccagcac cctgtttgaa ctcatcattc caaatcggct gaaggaatat gccccggacc
      481 gccgcaagat ggtgttggtg ctggacgaga agtctgccgc ctatccttgg gaattgttgc
      541 acaatcagta tgatcctaac tcccgtccat tagcggtgga ggccgggatg gtccggcaat
      601 tggcagttcc tcaattccgt gaaaaggtcc tccatgggac tgccctcaat gcattggtca
      661 ttggagaccc cacgagtcac gaaccctccg gaaaatttgc ccccttgccg ggtgctgctg
      721 ccgaagcgcg ggaagtggcg caactcattc gtcaccagga gtaccgggat gtggtggaac
      781 ttgttgagga ggaagcgaca ccggatgcga ttctgactgc cctgtataag caaccttacc
      841 gcattgtgca ttgtgcggcg catggtgtct ttgaatttcc aatagaagat gaccctgcca
      901 agcaaggcac ggcagactcg caaaaggcct ctggaacgcc acccaggatg gtcacgggaa
      961 tggttttagg ggaaggcctg tttttgaccc cgaccgaaat taatcaaatg cggtatgtgc
     1021 ctgagttggt atttttgaat tgctgccatc tgggacaaac caaaggcagt gctgaacagt
     1081 cgaacattcc atttcatcaa ctcgcttcaa acctcgggac ccaactgatt cgcatgggtg
     1141 tgcgggcggt ggttgcggca ggttgggctg tggacgacat ggccgctaaa accttcgcca
     1201 agaaattcta tgaagagatg ttccaaaacc gaacattcgg tgatgccgta cagctggcgc
     1261 gtgaggagat tttccttcgc cagggcggat ccaatacctg gggtgcgtat caatgttatg
     1321 gggatccgga cttttcgcta cacatcggag cgaaaagcat gggtatacaa cgccggatgg
     1381 tggctcctgt cgaattgcga gtggaattgt acaatctggt tcaagaggcc aaaacagccg
     1441 agccgaaaga tgaagcccgg ttgcgacgcc gattacaaga attggttgcc ggtgtcggac
     1501 aagggtggac ggattctgcg gccatgtgtg cggccctggg gttggcttat ggggaatttg
     1561 gcctcttcgc ggaggcggtt cgattctatg atcgagggag gatgctgcaa ccggccgatg
     1621 cgacggtgga gtcgctggaa cagttagcca acctgaaagt ccgatgggcg ctccagcgtc
     1681 tcgaaaggca ggcagatccc acagcacaaa agctcgactc actggaaaaa gattttccac
     1741 tcaaggaact ctttgacgat gcggaacata tactcgcagg ccttctcacg attcaacaaa
     1801 cacaggaacg gtatgctctc aaagggaagc tctataaagg aaaagccatg ctcttcagca
     1861 cgaaggcgga gaaacggaaa gctttactgg agatgaagca ttgttatgcg gagggctata
     1921 acattggcaa ggatgcccag cgacatgatg tgtattatcc cttggggaac cggctcgcgg
     1981 ctgagattgt gctctcgtgg gatcagccta aagggcggtc aacgcgacgc gggaagacca
     2041 aaggagctga tccaatcgca gaggggcttg ccgaattatc agtctatgca aaggaactaa
     2101 tcggaaaagg acaatccttc tggggtatga gtctgagccc ggatcaaaaa ctgctcgagg
     2161 cattatatgt acggcgtctg acagccaacg atcaaaagga aattctcaat ggataccttg
     2221 aggccaaacg acggggaggt tcagccagag aaatagattc cgtgatcaaa aacatcaggt
     2281 tctttgaatc gatggttgcc actcaggccc ctccgaacat tcgtcaacag ctgagcgccg
     2341 gactgaaaac attacgtgag agtctggtgt ccgatggtgg cgcaaatggc gagcctgaat
     2401 cgtaatgggt gtttcagatg aaaggcaatc gcctctgctg ctcccttggg aaattgtccg
     2461 atacaccagc tgttttcctc aacgccatgg gatccttcta tttacagctc tcgatcaaat
     2521 ggacctggtt gatccggccg gtcctggggt tcttgaccgg tgtgtgaagg gattttggat
     2581 acaccactga taatggagta tttgagatct ggtcacagta tatccaggtg tcccggcttg
     2641 caagtcctgg agcgggtgct cgcgcaaatc agttgacgga gaataccgct ttaaaaagaa
     2701 catccgcttg agaagaatat gttctgtatc catgtgtgat ccggtttctg aaatcctggc
     2761 gaattgagca aaaagtcaga tacattttga aaaatgcaga ccagagattg gatcaatggc
     2821 atgcttgatt ttgccatgaa cggcattcgt aaaagagcac gacttccgca
//
LOCUS       DATIAL010000320         9307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_704510, whole genome shotgun sequence.
ACCESSION   DATIAL010000320 DATIAL010000000
VERSION     DATIAL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704510"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..447)
                     /locus_tag="VLA60_09975"
     CDS             complement(325..447)
                     /locus_tag="VLA60_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09733.1"
                     /translation="MTIHLRILFSHRYRTVMEGRQADALLGLGENEVVRLDGTE"
     gene            826..1788
                     /locus_tag="VLA60_09980"
     CDS             826..1788
                     /locus_tag="VLA60_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09734.1"
                     /translation="MKLNVLFNVAVMLGMVAINSSAWGHSPNDAGEAGLQSKVREAGL
                     IMYGTVVDIQYRNSEPTKEQPQGLPHTFVTYQVLEVLRGDVPDKKVTLRIPGGADGQG
                     GVYMVSTAPAFALGQTDVLFVKGGEIDDCQLVECVEGRFRIHENQVLNGWGVPVVEAH
                     KTLRIGGKPRFDLNVMEIPRPSFKALLERPEMKAYIDRVSRESRQSLKDLQARYEREA
                     PKVTTVNLGNQILPIQKDVAEEPEAMPMEAYGDPLTPEVFFDAIREWNKQVGDPQFPI
                     VMANVKERFEVPDQEVLALEAKDEAVLKISDEERYDIHAKEGLQ"
     gene            1788..2888
                     /locus_tag="VLA60_09985"
     CDS             1788..2888
                     /locus_tag="VLA60_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HSF09735.1"
                     /translation="MKMTKILVPMILVVLCSSPAFSASWLECNGDSGKKLRWGGNSTT
                     ARINTGSFPAGSVLQAAQRGVNITNTNPSPFMINHTTETGGVGSGNGQNEIWAASISP
                     PGEARMRYHCYWLFGWHYGLDEVDIVLDSTGRSWTTSQNKNANFTYTGSSRPIDAVIV
                     HEAGHYLGLMHVNWEYNVMGDSWRHHHTNGGSAITYFGEDASHGARVLYGSQSSSFND
                     VAASHWRRTGASGEYSSHDRVRVRNSANTGTLTGITIAGEPGYRVNRGSVVRPEFTIE
                     NNGKQTHSNVTFGIYVSINDFISYSDTRIGGGTFGSIHPADVLTTTIPVIIPNYLNAG
                     QNYWLGIIVDEDNDINEVNGSNNRAYIPIRVQ"
     gene            complement(3168..3386)
                     /locus_tag="VLA60_09990"
     CDS             complement(3168..3386)
                     /locus_tag="VLA60_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09736.1"
                     /translation="MNDTMTISRSCEEGMYLFREGDYVWRVNEDGKVDCAVLGHTTVF
                     ELLTFIQKWKTPSRRIFQDYPFQDFLTD"
     gene            complement(4972..5508)
                     /locus_tag="VLA60_09995"
     CDS             complement(4972..5508)
                     /locus_tag="VLA60_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09737.1"
                     /translation="MRKMLLVLASILFITHFSFALAADPNMGKLQPPSMAPQRGGTIP
                     QLQLTPDYLYKQITALQQQVAGLQAQVNALRSVVQVTQNGATIQAAYLTINGQNNLAM
                     ISSKKINLTAGDDLTLTSGKDMSLQSQKDVTAEGAGKIRLKAPQIKLNDGTQPLAVVG
                     SSVSGGKVINGSVSVFVK"
     gene            complement(5985..7157)
                     /locus_tag="VLA60_10000"
     CDS             complement(5985..7157)
                     /locus_tag="VLA60_10000"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HSF09738.1"
                     /translation="MKILSFLRLGLLWYLVSLSPALLWAAPPNVVLSRALHFSSPDGT
                     PITIEPGKYFIEQAGPSKMRVIPEGDKPGLTIQAESLTHEQYELFSPMALTRPQKNEE
                     VLIELLLPGGIRLEARGSTKAPAEPPKIPVVSSPPPSPLTQAPEAAASVPPPVPMSPP
                     SIQEDTPATLASPAGDSSAMTKSLDTFFPYHAPQVSAQGTRVVVDEQEPGKDPLYLSV
                     LSPDHIGLTMFEQPALFWFIAQLTSQPVDVMIMDEGNTQILLDVRMLPPIQPGIHKVD
                     LKDYGIRLQLNTTYQWKIVLQGLNPGESRTTNGWIMRVSPSASVATLANLPSINATPT
                     EPLLEAGLWYDAIWALSERLHNDPTDTRALTQRANLFEQVGLLPPAKQDRAHLPLP"
     gene            complement(7704..7898)
                     /locus_tag="VLA60_10005"
     CDS             complement(7704..7898)
                     /locus_tag="VLA60_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09739.1"
                     /translation="MVTGLRKNSTISLSTWGQRGTAGLVEEWEPRIPIPEEKQQTRRR
                     SSISSPDSIEPTTERKNLKK"
     gene            complement(8365..8712)
                     /locus_tag="VLA60_10010"
     CDS             complement(8365..8712)
                     /locus_tag="VLA60_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09740.1"
                     /translation="MTVSLPQTLERTTDINSVTILLIDDSPDNLMILGDILEQMGYQV
                     VCAEDVEKGLEHAQRVQPDLIFMDVKMPGLDGFQACERLRAQENFQLTPIILMSAQSR
                     NHSSRQGLRSWRL"
BASE COUNT         2423 a         1983 c         2470 g         2431 t
ORIGIN      
        1 aaatatactg tgagcgtgtg gtgccgatgt acgcttgaaa agctgaatca atatggggct
       61 attgctctca cgtgcttgtt acccccggca ctccgagatt gtcctgaaaa gaggtttctg
      121 ccgattgtct gatgggttct cgccttccgt ccatgagcta tccccgccta ttgggataat
      181 gacttcgatc gcatcctcag tgagcgactt ccggcatgat ttccgttatg aatggcccaa
      241 agctgtataa ccggtttggg ggctatgccc ctctcgcgct ggtatcgttt cattgcgctt
      301 ccaccagtca gtgaagcgaa accgttattc cgtcccatcg agccggacca cctcgttttc
      361 gcctaatcct aataacgcat ctgcctgtct gccctccata accgtccggt atcgatgaga
      421 aaaaagaatg cgcagatgga tcgtcactca agaaatcggt gtgcgtatct aaaccgatac
      481 cattcgtatc tcatcttgtt tccttttctc ggtcaagaga aattcctcga agcgcttctg
      541 gattgaaatg tgccctaatt gcctaaaact agggtgaatt ttcggtagta gaaatagatt
      601 tcgtcaattg aaggttctgg cattgaggtt gctttagctg gctgcagaaa tattttttct
      661 cattaactca ccaaggaggg tgcatgctta gaaagcaggc gcacaagatt cagaggcaac
      721 gcaggaaatg gacatacaat gttttcggtg atccgtaaat gcagactgat tcgtcacggt
      781 tccggcaatt ttgtattcac gcaaaggaaa cagaaagggc attctatgaa actcaatgtg
      841 ttgttcaacg tagcggtaat gcttggcatg gtggccatca attcatcagc atggggacat
      901 agtccaaacg atgccggcga agccggtctt caatctaaag ttcgtgaagc gggtctgatt
      961 atgtacggca ctgtggttga tatccaatat cgaaactcag agcccacgaa agaacagccc
     1021 cagggtcttc cccatacgtt cgtgacttac caggttctgg aagttttgag aggtgacgtg
     1081 ccggacaaaa aagtcacgct ccgaattccc ggtggggccg atgggcaggg tggtgtctat
     1141 atggtgtcga cagcgcctgc gtttgccctt ggacaaactg atgtgttgtt cgtcaaggga
     1201 ggagaaatcg atgattgtca gctggtggaa tgtgtcgagg gaagatttcg tatacatgaa
     1261 aatcaggtgt tgaatggttg gggagtgccg gttgtggaag cccacaaaac gttacgcatt
     1321 ggggggaaac cacggttcga tctcaatgtg atggaaatac ctcgcccttc ctttaaagcc
     1381 ttgctggaac gccctgaaat gaaggcctat atcgatcgag tgagccggga gagccgacag
     1441 agcttgaaag atctgcaggc ccgctatgag cgggaagctc caaaagtcac gacggtcaat
     1501 ttaggcaatc agattctgcc aatccaaaaa gatgtcgccg aagaacctga ggctatgcca
     1561 atggaagcct acggagatcc tttgactccg gaagtatttt ttgacgccat acgtgagtgg
     1621 aataaacagg tgggcgatcc tcaattccca atagtgatgg ctaatgtgaa agaacggttt
     1681 gaagtgcctg accaggaggt cttggctctc gaggccaagg acgaagcagt acttaaaata
     1741 agcgatgaag aacgctatga catccatgct aaggaggggc ttcaataatg aaaatgacaa
     1801 aaattttggt acccatgatt ctcgtggtgt tgtgtagttc tcccgcattt tcggcatcct
     1861 ggctggaatg caatggggac agtggcaaga aattgcgttg gggaggaaac tccacgacag
     1921 cgcggatcaa tactggaagt ttcccggccg ggagtgttct gcaggcagcc caacggggtg
     1981 tgaatattac gaacacgaat ccctcgccgt ttatgatcaa ccatacgacc gaaaccggtg
     2041 gggtgggaag tggaaatgga caaaatgaaa tttgggccgc aagtatttct ccaccgggag
     2101 aggcccgcat gcgctatcac tgttactggt tgttcgggtg gcattatgga ctggatgaag
     2161 tggatattgt gctggattcc acagggcggt cctggacgac ctcccaaaac aaaaacgcga
     2221 attttactta taccggctca agccgtccca tcgatgcggt catcgtgcac gaggccggac
     2281 actatcttgg cctgatgcat gtcaattggg aatacaatgt catgggtgat tcctggcgcc
     2341 accatcacac caatggggga tcagctatca cgtatttcgg ggaagatgct tcgcatgggg
     2401 cgcgggtgct ttatggctcg caaagctcct cctttaacga tgtggccgcc tcgcactggc
     2461 gaagaaccgg agccagcggc gagtattcaa gtcatgaccg ggtacgggtc cggaatagtg
     2521 ccaacaccgg gacgttgacg ggtattacga tcgccgggga gcccgggtat cgggtgaatc
     2581 ggggaagtgt agtacggcct gaatttacca tcgaaaacaa tgggaaacag actcattcca
     2641 atgtgacgtt tggcatctat gtctcaatca atgactttat tagttatagc gatacccgta
     2701 ttggtggggg aacctttggc tcgattcatc ccgccgacgt gttaaccacc acaattccgg
     2761 tgatcattcc caattatctg aatgcgggac agaattactg gctgggaatc atcgttgatg
     2821 aggacaatga tatcaatgag gtgaatggtt caaataatcg tgcgtatatt cccatcaggg
     2881 ttcaatgaaa aagcagaagg tcgtgagtga tggatctcgg gacaatactg cttgtggttt
     2941 ctgattatct ataggggaat gggcttcgat cagatccccg ggcatgttca atgggacgtc
     3001 gatttgggag ccgaggccgt caggattcgt agaatcttgc atggcctcgg cttttcaatg
     3061 atgttcacgt gggcttgtca aaagtgatcg gtgtcaagca tgtagccgaa gaaccaggca
     3121 gtcataagca gggtgatgag aattggaggt cggggatgaa agcccggtca gtcagtgaga
     3181 aagtcctgga agggatagtc ttgaaagatc cgacgagagg gagttttcca tttctgaatg
     3241 aaggtgagaa gttcgaaaac agtggtatga cctagaacgg cacaatccac cttaccgtct
     3301 tcgttcactc tccacacata atctccttcc cgaaatagat acattccctc ttcacatgat
     3361 cgactaatgg tcattgtgtc attcatctct tgtttctcct ttagaagccg taggccacag
     3421 aatccgccaa aaaattgacg cggtgagaat cacagactcc tttgttcgca aaagaaatca
     3481 gcaataaaat gttcagaccc catggaatgc aaatatccac caccatacaa tcgatttgga
     3541 tagatgggta ataagtcatt caatctgact ggttgaatcg ggagtatgga ggtcgtgagg
     3601 aaaggcatgg tatgggatgg acgtttatta tagggttcat gcctatggtc gccacaacca
     3661 atataaagaa accaaaccca acagacttac gcaactgatg atgagtaaaa taccttccat
     3721 ggtgttgcct ctctctttgg tcagtcggct ttcacccctt cctgattcca aataaaagca
     3781 accttgatgc cgttggtcgt gttgtggctc ctggccaacc aggattgcaa cctaaacaat
     3841 taattttaat gagaaaagca aagagagaat acatcatggg tcctggaaaa cttcgactgg
     3901 tgtgacgcgg ggaagtaccg aaacgggtac atgccgtaaa cattggtggc agatacgaat
     3961 atactgaccg tttcgaggcc tatttatgaa ataatgagga agggggaatg gtgccaatcc
     4021 ggcattgaac ctcaataagt gagggttctg ctgtcttgga aacccattac gaaggccttt
     4081 ttaaaaagaa tctgcctact tctacaagtg agtggaggaa ggcctgttgc ttattcgcaa
     4141 gccaccgctc gggtttttcg aggggtatgt aatagcgtaa taattctaag tttttatccg
     4201 cagattttct ggcagccagg ccttgcaata agtatgataa gaaaatcgat gctgtttttt
     4261 tatcgcggtg aatcgaactc gtttcgacaa aaaaacatcc cgccaggaac aaataggacg
     4321 gatcgcacaa ccattccttg caggacttga cccgccggac aactcgaact gggagcgttg
     4381 ttcccacccc gcccactggt cccctggcaa actcattttc caatattccg tcttaacagg
     4441 tttcatgctg gcataggagt cccaatacat ctcgctccat ttcctaccgt gacggcatca
     4501 ttgccacaat ccaaaatata ggaatggttt catacgctgc ctccaaagct gggcattgag
     4561 aattccgctg aggcggtact cggcgaagtg gaagggaggg tttttaaacg ttgaatggtc
     4621 aagagaagtg gtcgctttgt tttgcatgtc gcagaaactc cagagggaat cccaaccctt
     4681 tacccattgg cagcagaaag gctaagtggt gagtatcaag ttgtttttcc tgaatggacg
     4741 cgccagcggg gcggttgggg tttttcagtc ttggtgctgg acatgccatc aaccggaaat
     4801 atcgtgatga aggtctttct tgtttctcat gagtcttagg cctggtgttc tttcaatcgc
     4861 agtccggcaa tggggaaaat tttaactgcc tcgtgaaaga cagagggctc cccgggactg
     4921 actccttttt cttctgcctg ccaattgaac ctcgaggaat cgccggattt attatttaac
     4981 aaagacgctc acactcccat tgatgacttt ccctccggag accgagcttc cgacgactgc
     5041 cagaggctgg gtgccgtcat ttaattttat ttgtggagct ttaagtctta ttttgccggc
     5101 gccttcagcc gtgacatctt tctggctttg taaggacatg tcttttccgc ttgtgagggt
     5161 caaatcatct ccagcggtca gattgatttt ttttgaagaa atcatggcca gattgttttg
     5221 gccgttgatg gtcagatatg ccgcctgtat agtcgccccg ttttgagtaa cctggacaac
     5281 cgatcggaga gcgttcacct gggcttggag gccggcaacc tgttgttgga gtgccgtgat
     5341 ttgtttgtag agataatccg gtgtcaattg aagctgcgga atggtccctc cccgttgtgg
     5401 ggccatggag ggcggttgga gctttcccat gttcggatca gcggcgagag cgaatgaaaa
     5461 atgagtaatg aacagaatgc ttgcaaggac caataacatt tttcgcatga aagacctcct
     5521 ttggtttcaa cagcctgccc tttgaagaga gccgatgtgt tgtttgagga cggaaatctg
     5581 tttgttgagc tgggaaattg ctgtatgtgc aagggatgat gccaggaata gcgccgtgaa
     5641 tgtcgaaagg atcagaacac gcatggagtt tccttacatg gtcgtctttt atctcaggtc
     5701 tgacgctgtg gaagttcttg tggggtctgt cgtgccaatt aagctcaagg ttcacgcctg
     5761 gctgattggt tgactctgat gaacgggaaa aatctgttgc aagtgtaccg atgccaggca
     5821 aaaaaagtaa ggaaatggct gattttgttg accgttatgg ggaattttcg cgacaacaag
     5881 tcatggttac gtatgtttag ttacaggtaa gtgactggaa ggaaaacaat gatggaatcc
     5941 acctgagcag gaaaattccc tcaacgcggc actactggtt ttttctaggg aagaggaagg
     6001 tgagcacgat cctgcttggc cggcgggagg agaccaactt gttcaaaaag attggctcgc
     6061 tgtgtgagag ctcgggtatc ggtgggatcg ttgtgaaggc gttctgataa ggcccagatg
     6121 gcgtcatacc agagccctgc ttcaaggagt ggttccgtgg gagtcgcatt aatggatggg
     6181 agattggcaa gagttgctac gctggccgaa ggcgagaccc gcatgatcca cccgttggta
     6241 gtacggcttt ctcctgggtt caggccttgg agaacgattt tccattggta ggtcgtattg
     6301 agttggagcc tgatcccata atccttcagg tctaccttat gaatgccggg ttgaatagga
     6361 gggagcatgc ggacatcgag cagtatttgt gtattccctt catccataat catgacatcc
     6421 acgggttgtg acgtcagttg ggcaataaac caaaaaaggg cgggttgttc aaacatcgtc
     6481 aatccaatat ggtccgggga taagacagac aggtacagcg ggtcttttcc cggttcctgc
     6541 tcatccacga caacccgtgt gccttgggca gaaacctgag gggcatggta cgggaagaag
     6601 gtatctaaac tcttcgtcat ggcagaagaa tcaccagcgg gtgaggccaa ggtagccgga
     6661 gtgtcctctt gaattgatgg aggagacata gggacaggag ggggaacact tgctgcagct
     6721 tcaggcgctt gagtcaatgg ggatggagga ggagacgaga ccacggggat tttgggtggt
     6781 tcggcaggag ccttcgttga tcctcgggcc tccaggcgga ttccgcccgg taaaaggagt
     6841 tcaattaaga cctcttcgtt cttttgaggc cgagtgagag ccataggaga aaacaattcg
     6901 tattgctcgt gagtgagaga ttctgcctga atggtgagac ccggtttatc cccttcaggg
     6961 atgacccgca ttttagatgg tccggcttgt tcaatgaaat attttccggg ttctatggtg
     7021 atgggagtgc catcgggaga agaaaagtgg agtgcgcggc tcaggaccac attcggaggg
     7081 gcagcccaaa gcagagccgg agacagactc accagatacc acaacagtcc aagcctgaga
     7141 aaggagagta ttttcacaaa cgtatcgtta gaatactgta aaaatagaat tttaaggctc
     7201 taccttacgg gaatcgtaaa agattgtgca atcagaagca tgcgagaatc cacttggaat
     7261 gaagatgtgg ctgggtaatt cctgagatca gtcctagaaa acggaaggct gataattcta
     7321 catgaattga tagctttcgg ccgagccata tttgacctcg gacgatatcg tttccctcaa
     7381 aaatacttct attgtcagtc ggcctcagtc aataattgaa tcctcggcag gttgttcctt
     7441 tctgtttcca gaacagatct atttaagagc aatgtggtca agggaagacc cacattgtcc
     7501 gggataaatg atatttttcc tggactccga gaaaatattt tccggagagg gtggtagatt
     7561 cttatgatga ggaggggctt gtagaacgtc actccgttag aaggctcaac atttgtcacc
     7621 cgaaacccag tccatgggag atggctggat acatcactta tacgaagaag attgagcgag
     7681 tcttcttgag ggggggagcg tccctatttc ttaagatttt tcctctccgt ggtcggctcg
     7741 attgagtcag gactggagat ggatgaacgt cgacgggttt gttgcttttc ttcaggaatg
     7801 ggtattcggg gttcccattc ctcgaccaac cctgccgttc cccgttggcc ccaagtggac
     7861 aggctaatag tagaattttt tcttagaccg gtgaccatgc gtactttctc caggttgtaa
     7921 aaggtaaata agacaaattg gcgtctgtta aatgactctt tctgtggggt tttcccttca
     7981 aaaatgtatc ttaaatggag aattttgaag gaaaacccac ataaagaaat taaacagcaa
     8041 tttagatgcc atgtcaaaat ccggtcttta tatcagggct tgtagcaatg atttgagcaa
     8101 tggggcaggg gagtgggggc ttcccatgtg agtgggattt tgtttatttg gggaagatgc
     8161 atgtatccat ttagataaat aagtgtattg aatgggaaat tttctagtat caatagaaag
     8221 agggaggtcg ccccttaggg gcacatcccc actgaattcc gtttgcagtt aatgtaaacc
     8281 ccgatctaac ttgaacctta ttatattaaa aatcgaggaa gttcggtccg gattttcgtg
     8341 ggggttatgg gtttaagcca aaactcagag cctccagctg cgaaggccct gtctcgatga
     8401 gtgatttcta gattgcgcgc tcatgagaat aatcggggta agctgaaagt tctcctgtgc
     8461 tcgaagtcgt tcacaggctt ggaacccatc aaggccaggc attttcacat ccataaatat
     8521 caagtcgggt tgaacgcgtt gggcgtgttc aagtcccttc tcgacatctt ctgcacaaac
     8581 aacctgataa cccatttgtt ctaagatatc tcccaggatc atcaaattat caggggaatc
     8641 atcaatgagc agtatggtga cagaattgat gtcggttgtc ctttctaatg tctgtggtaa
     8701 agataccgtc atgttcatcc tgtcttcctc catttcttgt cgtattgctt catcaatttt
     8761 ttttaccgat gaattacgca aggagagtgc cggattgatt ggacattgta acgcattgaa
     8821 attgagctat ttttagttaa tctatctcaa gggatcgggt gtcatgaggt gtgtcatgag
     8881 acaaagagga tgggacagga aaggtgtttg agacaatgaa aaaattcgca atggcgagaa
     8941 gaaacctggt cacaaatgga tgctatcagt cagttcggat tgatcactcc aacagataat
     9001 tgtgagatct cctagtaaaa aattagagat tctcttggcg gtaaatttcc tccccaaaaa
     9061 atgagagggg ttttctggac gttcatggta ggctaccgga aatctcctca gcaattgatt
     9121 acaggcagcc atgcccgctg cggtggaaag agatatggaa cagcttcaag atacaataaa
     9181 tcaaaaaatt caattggtga gtattgttat tccggtcttc aacgaacgaa aaacgattga
     9241 gacgattgtc gggcgcgtgc aaaatgtgga aattccaaaa gaactaatca ttgtggatga
     9301 cgggtcc
//
LOCUS       DATIAL010000321         2918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1134564, whole genome shotgun sequence.
ACCESSION   DATIAL010000321 DATIAL010000000
VERSION     DATIAL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134564"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VLA60_10015"
     CDS             <1..498
                     /locus_tag="VLA60_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HSF09741.1"
                     /translation="HRVQRVPTTEANGRIHTSTATVAVMPEVEEVDVHIDPKDLRIDT
                     YCSSGAGGQSVNTTYSAIRITHIPTGVVVTCQDERSQLKNRAKAMRTLRTRIGDAERE
                     KQEASIAQQRRSQVGSGERSEKIRTYNFPQNRVTDHRIGLTLHKLDRVMEGDLDGLIA
                     GLKAQ"
     gene            573..1451
                     /gene="prmC"
                     /locus_tag="VLA60_10020"
     CDS             573..1451
                     /gene="prmC"
                     /locus_tag="VLA60_10020"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSF09742.1"
                     /translation="MPPFTSYSQIYRGAVDTLTQAGISNARHEALWILEYALGVPRLQ
                     FHLNPDTPVDIETCRLAGALVQRRATREPIQYVLGSQEFLGLDFIVRPEVLIPRPETE
                     LLVEETITLLRSETRPVILDVGTGSGCLAISLAVNLPQAMIFASDKSVAALHIARLNA
                     MRHGVSQQILWMAGDLLAHLLSKNLAGKVTAIIANLPYISHEEWDHLSPDVKDFEPRL
                     ALDGGPDGLDVYRRLLKEAPRVMSSEGFMIMEVGRGQADLLCRELSGTEAFNILKVRP
                     DLHGIPRVVSVQRRST"
     gene            1465..2724
                     /gene="murA"
                     /locus_tag="VLA60_10025"
     CDS             1465..2724
                     /gene="murA"
                     /locus_tag="VLA60_10025"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSF09743.1"
                     /translation="MDQIRINGGVPLKGVVEIGGAKNAALPILAAALLGGGECVIDHV
                     PQVRDVVTMRKLLALLGVNVHADGSRITLDATHVQSTEAPYDLVKTMRASILALGPLL
                     ARLGEAKVSLPGGCAIGTRPVSLHLKGLEMMGAEIQVEHGYIHAKVSRLKGTRIDLDF
                     PTVTGTENLIMAACLAEGTTSLHNVAREPEITDLCAFLTKRGAKILGMGTDTITIEGV
                     RELHGAGHSIIPDRIEAGTYLMAGAITGGDVMVKGCVTDHLGSVLSKVQEMGVELTES
                     PEGVRIRSSGPLKAVEVKTLPYPGFPTDLQAQVMAVMSVAQGTSVIAETIFEGRFLHV
                     PELHRMGASIEVDGPHAVVKGVPLLTGAPVMASDLRASAGLVLAGLAADGETNISRVY
                     HLDRGYERLEEKFQALGANIERVREGA"
     gene            2721..>2918
                     /locus_tag="VLA60_10030"
     CDS             2721..>2918
                     /locus_tag="VLA60_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSF09744.1"
                     /translation="MSEVITIALSKGKLLDPTLELFRQAGYTGYRVPEDDRRLILEYP
                     EHGHRVLIVRASDVPAYVEYGA"
BASE COUNT          678 a          662 c          868 g          710 t
ORIGIN      
        1 catcgagtac agagggttcc gaccacggaa gccaatggac gaattcatac ctcgacggcc
       61 actgtggctg tcatgccaga agttgaagaa gttgacgtgc acattgatcc caaggatctg
      121 cggattgaca cgtattgttc ctcaggggcc ggcgggcaga gtgtgaatac cacctattcc
      181 gcgatcagaa tcacgcatat tccgacgggg gtggtggtga cctgtcagga tgagcggtcc
      241 cagttgaaaa accgggcgaa agccatgaga acgcttcgga cgcgcattgg cgatgccgag
      301 cgagaaaaac aggaagcctc gattgctcaa cagcgacgat cccaggtcgg gagtggagag
      361 cggagtgaaa aaatccgcac gtataatttt ccacaaaatc gtgtgactga tcaccgaatc
      421 gggctgacgt tgcataaatt agatcgggtg atggaaggcg accttgacgg gctcattgcc
      481 ggtctcaaag cccaataacg ctttcattca tctctcttcc ttcattccat tatacataga
      541 tgtttaacgt gtgctctgtt ttggaggctg tgatgccccc tttcacttcc tattcacaga
      601 tatatcgagg tgcggtggat accctgactc aagccgggat ttccaatgcc aggcatgaag
      661 cactgtggat tctggagtat gcgctgggag ttccacgatt gcagtttcac ctgaatccag
      721 acactcccgt tgatattgaa acatgcaggc tggcgggtgc cttagtacag cgccgtgcga
      781 cacgggaacc cattcaatat gttttaggga gccaggagtt tcttggcttg gacttcattg
      841 ttcgaccgga ggtcctcatt cccagaccgg aaacggagtt attggtagag gaaacgatta
      901 ctctcctgcg ctcggaaaca cgtccggtga ttctggatgt cgggacaggt tcaggatgtc
      961 tggcaatcag tctggctgtc aatcttcctc aggcgatgat tttcgcctcg gataagtcgg
     1021 tcgccgcttt gcatattgcc aggttaaatg cgatgcggca tggggtctct caacaaattc
     1081 tctggatggc aggcgatctt ctcgcccact tgttatccaa aaatttagca ggtaaagtga
     1141 ccgccattat cgctaacctg ccgtatatct cccatgagga atgggaccac ctttctccag
     1201 atgtcaaaga ctttgagcca cggctggcgt tggatggagg gccggatggg ttagatgtgt
     1261 accggcggtt gttgaaggag gcacctcgtg tgatgtcttc agagggattt atgatcatgg
     1321 aagtaggaag aggtcaggct gatcttctgt gccgggaact gtccggcacc gaagcattta
     1381 atattttaaa ggtccggccg gatttacatg gcattccacg ggtggtaagc gtgcaacggc
     1441 gttcgaccta ataggaaata ttcagtggat cagatccgga tcaacggagg agttcctctc
     1501 aagggtgtgg ttgaaatcgg cggggccaaa aatgcggcct tgcctatttt ggcggcagcg
     1561 ttgttagggg gtggagaatg cgtgattgac catgttcccc aagttcggga tgttgtgacc
     1621 atgcgcaagt tattggcctt attgggcgtc aatgttcatg cggacggttc ccgaatcaca
     1681 ctggatgcca ctcatgtgca atcgactgaa gcgccatatg atttagtgaa aacgatgcga
     1741 gcctctattt tggccctagg tcccttgctc gctcgattgg gagaagccaa agtttcgttg
     1801 ccgggtggtt gtgcgatcgg cacgagaccc gtgagtcttc atctcaaggg attggagatg
     1861 atgggtgccg agattcaagt cgagcatggc tatatccatg caaaagtttc ccgtctcaag
     1921 ggaacaagga ttgacttgga ttttccgact gtcacgggaa cggaaaattt aatcatggct
     1981 gcctgcctgg cggaaggaac aaccagtttg cataatgtgg cgcgagaacc tgaaattacg
     2041 gacctctgtg cgttcttaac gaagcgggga gccaaaattc ttggtatggg caccgacacc
     2101 ataaccattg aaggagtgag agaactccac ggggcaggac atagcatcat tcctgatcga
     2161 attgaggccg ggacgtattt aatggccgga gcgatcaccg gtggtgatgt gatggtgaaa
     2221 gggtgtgtga cggatcattt aggcagcgtc ctgtcaaaag tacaagaaat gggtgtggag
     2281 ttgacggaaa gccctgaggg ggtccggatc cgctcgtcag gcccgctgaa agccgtggag
     2341 gtgaaaaccc taccgtatcc agggtttccg acagatctgc aagcccaggt gatggccgtg
     2401 atgtcagtgg cacaaggcac cagtgtgatt gcggaaacta tttttgaagg acggtttctt
     2461 catgtcccgg aacttcaccg gatgggagcg tcgattgagg ttgacgggcc acatgcggtg
     2521 gtcaagggag tgccattgct gacgggtgcg ccggtcatgg cttcggattt acgcgccagt
     2581 gcgggcttag tgttggccgg tttggcggct gatggagaaa cgaacatttc cagggtttat
     2641 catttagacc ggggctatga acggctggaa gagaagtttc aggcgttggg tgccaacatt
     2701 gaacgggttc gtgagggtgc atgagcgaag tcattactat tgccctttcc aaaggcaagc
     2761 ttctcgatcc cactcttgaa ttgttcagac aggcggggta taccggttac cgtgtgccgg
     2821 aagatgatcg acgattgatc ttggaatatc ccgaacatgg tcaccgggtc ttgattgttc
     2881 gagcaagcga tgtgccggcg tacgttgaat atggcgcc
//
LOCUS       DATIAL010000322         4613 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1242455, whole genome shotgun sequence.
ACCESSION   DATIAL010000322 DATIAL010000000
VERSION     DATIAL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242455"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VLA60_10035"
     CDS             complement(<1..392)
                     /locus_tag="VLA60_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF09745.1"
                     /translation="MRFSKKSEYALRAMMELTQEFGQGLVQRKSLAERQNIPLGFLEM
                     ILLSLKNAGLIGSRRGAHGGVFLIKSPREVTLGQIIRLLDGPLAPIACVSQTAYQKCQ
                     DCPHAGTTACPIQRIMLDVRNAIASVLD"
     gene            complement(717..980)
                     /locus_tag="VLA60_10040"
     CDS             complement(717..980)
                     /locus_tag="VLA60_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09746.1"
                     /translation="MSFFPVLNRPGWIPGDFSEIPVEVFEIRLRKECPEMGWGSWKIL
                     HTGAWKAPGMRYDWRGNSLVCCTTLTKNCKKSEFVLALGAENA"
     gene            complement(1377..2498)
                     /locus_tag="VLA60_10045"
     CDS             complement(1377..2498)
                     /locus_tag="VLA60_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSF09747.1"
                     /translation="MSLNKTSSSQKLRGKTDQSLEEERVKTDEFLEQNVKSVEEETEE
                     TIRLTRLAADKEVESHRAEVDLEREQRLDGTDTGQLVDSLFTQERERADEVHTVERVE
                     EDRARTQEQFQKQMIAEALLEKERKETDFNLLDERIHADLELITRDQFLTIVSHDLKN
                     SIVAISLVARGIRSGLSKDPVDTVGLLKNLGIIEEATAGMNRMISDLLDVEQMAHDKL
                     QLEPERVDLLVLLQECVGQFAPIVSNKSFSMTIDTSPGPIFADLDHDRILQVLSNLIG
                     NSLKFTPNGGTIELSARKQETHVEISVTDNGPGIPEQLQAQIFERFSQLKLNDRRGIG
                     LGLFIAKWIVEAHRGRIWVTSHPGKGSTFSFTLPLSVSE"
     gene            complement(2948..3148)
                     /locus_tag="VLA60_10050"
     CDS             complement(2948..3148)
                     /locus_tag="VLA60_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09748.1"
                     /translation="MKYGVRAIGLCWCLFGLIACGGTVKKSFTNPAYPLSKVSSIAIL
                     PFDGVGEAGAASDLLAMGLAVH"
     gene            complement(3653..3728)
                     /locus_tag="VLA60_10055"
     tRNA            complement(3653..3728)
                     /locus_tag="VLA60_10055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3693..3695),aa:Ala,seq:cgc)
     gene            complement(3901..4521)
                     /gene="purN"
                     /locus_tag="VLA60_10060"
     CDS             complement(3901..4521)
                     /gene="purN"
                     /locus_tag="VLA60_10060"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HSF09749.1"
                     /translation="MSLALGVLISGRGSNLVAILDAIDRGSLDATLKVVASNKPNAAG
                     LERAKERGLPTIYLDPKPFAQEAKPREAYDLAMGALLQQHGVEYVALAGYMRIVTPAL
                     IRMYPSKIFNIHPSLLPAFPGLDAQRQALEWGTKVSGCTVHLVTEGVDEGPIIVQATV
                     PILEGDTVDSLSARILEKEHECYPQALQLMAQKRLHVEGRKVHILS"
BASE COUNT         1173 a         1186 c         1073 g         1181 t
ORIGIN      
        1 tgatcaagga ccgatgcaat ggcgttccgc acatccagca tgatgcgctg aatcggacac
       61 gccgtcgttc ccgcatgagg acaatcctgg catttctgat acgccgtctg actcacacag
      121 gcaatcggtg cgagtgggcc atccagaagc cgaataatct gccccaaggt gacttcgcgg
      181 ggagacttaa ttaaaaacac cccgccatgg gcaccccgac gactcccaat cagtcccgcg
      241 ttctttaacg acaacaaaat catttccaga aagcccaatg gaatattctg ccgctccgcc
      301 agggattttc gttgcacgag tccctgcccg aactcctgag tcagctccat catggcccgc
      361 aaggcgtact cacttttttt agaaaatcgc ataatagttt attaagttaa ctaaagttat
      421 gcacatttaa cataactacg ttcaggtttc tagaaaacac acagagattt ttgacagaga
      481 tgcgaagaca ggcaggaaat gtcaggatgg cgtcaaatgc ttcggtacag gtctatcggc
      541 aaaacgaaag tctctatcca gacttcgaaa accgtgaaga gtccctcacg ctccctcacg
      601 gtggatcaca taagtcaaat ctcctaccct aagccagcga tatccctacg attcatgggc
      661 cattttatgg atgccggaag gaggactggg actttcttcc tccttcatta ggttcactag
      721 gcgttctccg cccctaacgc caggacaaat tccgacttct tgcagttttt cgtcaaagtt
      781 gtgcagcaca ccaaggagtt gcctcgccaa tcataacgca tgccgggcgc tttccatgca
      841 cctgtatgaa gaattttcca actgccccat cccatttccg gacattcttt gcgcaagcgg
      901 atttcaaaga cctcgacggg tatttcagag aaatctcccg ggatccatcc tggtcgattg
      961 agaactggaa aaaatgacat ttctcaatgc cagcttactg gatatgccaa ctttactttt
     1021 ttgagccggc cacccccatg tcctgttgcc actgtagatt cacggttggt tcgattgaaa
     1081 aacggaactt tctcggcagt tgtcatgaat agtattcacc gtttttgcga agaatggcga
     1141 aagtctctga aggaacattt tttaaagctc tgcatggttc accctcaaat ccctgcgcta
     1201 acgccaaaag aaacctcctg acgacccggc tacaatggcc ttgccttata ggttcgtagg
     1261 agatttgaaa cacggtagga atgaatgggt ggagagcacc cagtacctgc tttattggcc
     1321 ccacacttcg tgtgccgctg agagctatcc cgttggttgc gaagaacacg tctatgttat
     1381 tccgatacag ataatggaag cgtgaagctg aacgtgcttc ccttcccagg atgtgaagta
     1441 acccaaatgc gccccctatg ggcttcgacg atccacttgg caataaaaag cccgaggccg
     1501 atgccacgac gatcgttcag tttaagctgc gagaatcttt caaaaatttg cgcctgtaac
     1561 tgttctggaa tcccaggccc attgtcagtt accgaaattt caacatgagt ctcctgcttc
     1621 cgtgcagata actcaatggt gcctccattg ggtgtgaact tcagtgaatt tccgatcaag
     1681 ttggacaata cttgaagtat tctgtcgtga tcgaggtcgg caaatatcgg tccaggggat
     1741 gtgtcaatgg tcattgaaaa tgacttattt gaaacgatcg gagcaaattg acccacgcat
     1801 tcctgtaata aaaccagaag atcgaccctt tcaggttcaa gttggagttt gtcatgtgcc
     1861 atctgctcga cgtcaagtag atcgcttatc atccggttca tgcctgccgt ggcctcctcg
     1921 atgattccaa ggtttttgag aagacccacg gtatccaccg ggtctttaga aagaccgctc
     1981 cgtatcccgc gagcgaccaa cgaaattgcg acaatggaat ttttcaagtc atgactgaca
     2041 atcgtgagaa attgatctct cgtaataagc tccaagtcag cgtggattcg ttcatcgaga
     2101 agattaaagt ccgtttcttt tcgctccttt tctaggagag cttcggcgat catttgtttt
     2161 tgaaattgtt cctgtgtccg ggcacgatct tcttctactc gttcaacagt gtgaacctcg
     2221 tcagcccgtt cccgctcctg agtgaaaagg gaatcgacca attgtccagt atccgtcccg
     2281 tcaagtcgtt gttccctctc cagatcaact tccgcgcggt ggctttcaac ttctttatcc
     2341 gccgcgagcc gagtcagccg gattgtctcc tcggtttctt cttcaaccga cttaacattt
     2401 tgttcaagga attcgtcagt tttgactcgc tcttcctcta agctctggtc ggttttacca
     2461 cgcagctttt gagaggatga tgttttgtta agtgacatag attgaggcct cgctcgttgt
     2521 tgtcacaaaa attagagggt gaccaaataa gattttatac tatccgaata ggcacggcta
     2581 gcgagagcac cagccacaat gtcacacacg cgaaagccgt gtctgaaaga ggttcgtcgg
     2641 ggctggtaat ggcacacgaa agttttgcgg gctgatacag catattcttg cgcttacgcc
     2701 agttcgacag cgtctcggca gggtagaagc gggtggcgct tttccagaag tcctgatggt
     2761 ccaggatgtg accatcgctc gctcaaattg tttgggtagc gaggacagca tctcaacaaa
     2821 tggggtaaaa gcctccgggg tttcccccta actgccaaca cgcctcatcg ggaatcgcca
     2881 gagacccctt ctcgcaacgc atggcagaaa cattccgaaa cccggcttcc atcgtattac
     2941 gtgaatgtca atggacggca aggcccatag ccagtagatc tgaggctgca ccggcctcac
     3001 ccaccccatc aaagggaaga atcgcgattg acgatacctt cgaaagcgga taggccggat
     3061 tggtaaaaga ctttttgacc gttcctccac aggcaatcaa tccgaataga caccaacaaa
     3121 gaccaatggc tcgcaccccg tatttcatgt cgatcccttt atctcagaga cttattgaac
     3181 gtctggttgt cattacgaat tcatgctgga caagattgta ggcattaggt tttctgatgt
     3241 acaggtttaa aagaatattc tcggaatctt gtctgcgtaa tctctttgtt tgaagaggaa
     3301 aagtccagcc ctggtgattc tcggtcaaag cgtgaaacaa gaatctgaac caacacgacg
     3361 ccatttcctg gtttgtcgaa tcagaaagtc gattggttca aaggtccggt gtgggctcac
     3421 ctaagcggct ctatcaagca tagatgggca attgaatgcc ctaagagcct atggacgaat
     3481 cttcggtttt tgaaaagagg ctggccagga cgaatacaga tagttcgtca tacttaatgc
     3541 ggcgataaga agttgctaaa tctttttcgg acaaccgaca acaaaaaagc cgcctatgca
     3601 gcggcttttc atctcatcat tatactgtat cggatgtctt gggatgatga agtggtggag
     3661 ggcacgggag ttgaacccgc gacccctacg ttgcgaacgt agtgctctcc caactgagct
     3721 agccccccac acttgagatt cgtgttctgt gaagcgttaa aaaaaaagaa ggccttatgg
     3781 attgaaaaat atcacaggat tataactgac tgttagtcga gttgccatcg ctgacctgtt
     3841 tttttgcctg cccgcagtcc cctcacgctc ccattaatcg tttaggtctg ataatttctt
     3901 ctatgaaaga atgtgaacct tgcgcccttc cacatgcaat cgtttttgtg ccatcaactg
     3961 aagagcctga ggatagcatt cgtgctcttt ttccaaaatg cgagcggata aggagtcgac
     4021 cgtatccccc tcaagaatgg gcacagtagc ttgcacaatt atcggtcctt catcgacccc
     4081 ttccgtcacc agatgcaccg tgcaaccgct gactttcgtg ccccactcca aggcttgacg
     4141 ttgggcatca agtcctggaa atgccggtag aagcgaagga tggatattaa agatcttcga
     4201 tgggtacatt cgaattaagg cgggggtcac aatccgcata tatcccgcca gagccacata
     4261 ttccactcca tgttgctgaa gaagggcacc catggccaga tcataggctt cacggggttt
     4321 agcttcctgg gcaaaaggct ttggatccag atagatcgtg ggaagccctc gttcctttgc
     4381 acgctctaag cccgcggcat tgggcttgtt actggctacg accttgagag tcgcatccaa
     4441 cgagccacga tcaatggcat ccagaatggc caccagattt gatccgcggc ctgagattaa
     4501 gaccccaagg gcaagcgaca cagtttgaac cttctaatgc gaataggaaa gctttgcctc
     4561 agaagacggg cgatctcgaa cctctccaat gacacagccc gtcatccctt gtt
//
LOCUS       DATIAL010000323         5410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_919944, whole genome shotgun sequence.
ACCESSION   DATIAL010000323 DATIAL010000000
VERSION     DATIAL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919944"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..693)
                     /locus_tag="VLA60_10065"
     CDS             complement(238..693)
                     /locus_tag="VLA60_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HSF09750.1"
                     /translation="MIGIISDTHGLVRPQVIAALTGAELIIHAGDIGSPDVLKTLEAI
                     APVIAVRGNNDQDPWAAKIPLTNVVEHQSYFFYVVHELDHLDIDPVASELSAVIFGHS
                     HRASAERRKGILYLNPGSAGPRRFTLPISVARLHMTDTGLTPEIIEISK"
     gene            complement(729..1220)
                     /locus_tag="VLA60_10070"
     CDS             complement(729..1220)
                     /locus_tag="VLA60_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09751.1"
                     /translation="MVLVILLGDYLAGPTIQFPILYLIPVSLASWYRGFSWGLTFAVT
                     LSLIRLIFNIQFWSVSLTILDASINTVIRILVLGGAAFLVSRTAMQTRALANRVNVLE
                     GCLPICSFCKKIRDDNNNWQPLERFISDRSNAQFTHGFCPECGQRHYGEFFKTGDRPT
                     IES"
     gene            1801..3414
                     /gene="zwf"
                     /locus_tag="VLA60_10075"
     CDS             1801..3414
                     /gene="zwf"
                     /locus_tag="VLA60_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSF09752.1"
                     /translation="MAPDANDTSGKVSESVKPSHPVFDHADEMKETPLFQQPSNGQTR
                     PPTLFIIFGAQGDLTKRKLIPALYNLASSHHLPQEFAILGIDGIPMHTDDFREKIRQD
                     IDELCPRPIDATIKEWLLDRLHYLSGDFRDPQTYERLESLLTQLNTTHGTQGNYLYYM
                     ATAPTFFAEIVAQLGAKGLVEHRKDFSRRIVIEKPFGHDLPSARSLNQALRQVLDESQ
                     IYRIDHYLGKETVQNILIFRFANGIFEPIWNRQYIDHVQITVAETLGVEHRGAYYEKA
                     GALRDMVSNHLLQILAFVAMEPPNTFDPEAVRNEKAKVLRAIQPMNPVEVLQSTVAGQ
                     YEAGSINGHRVLPYRTEKDVNPASLTETFAAMTLGIESWRWEGVPFYLRTGKRLHTRV
                     TEVVIQFKSAPLMLFRKTPVDRLTPNVLVIRIQPDEGISLSFGAKIPGPKVKVGTVDM
                     DFQYAEYFGDAPSTGYETLLHDVMAGDATLFQRSDSVEVGWSIVDPIVKVWESLGSHA
                     IRPYSSGSWGPPEADALLAKDGRMWRNHG"
     gene            complement(3501..4934)
                     /locus_tag="VLA60_10080"
     CDS             complement(3501..4934)
                     /locus_tag="VLA60_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HSF09753.1"
                     /translation="MRRHQPASDISWTRDAIIYQIFPDRFARDTQQLKPGSGFVSWGS
                     KPTAHGFMGGTLRGITEHLEYLLDLGVNLIYLAPIFFASANHRYNTYDYYRIDPRLGT
                     LADFQTLLSRAHANGIRILLDGVFNHCGRGFFPFYDVMERGPASPFISWFFITRFPVN
                     AFGQFRYRGWQERPLMPILNLANPATRRYFLDVAAYWTNQGIDGWRLDAVADVRGHQF
                     WKALWQKVKTINPSSYMVAEIWGPGRSWIRDGQFDGGTNYVLRQVVLDYFIHGSISTR
                     QFVSRVHNLLRMYPWEKTLAMVNVLGSHDTERLYALARGNILRLKLAMLFQFVFPGIP
                     AVYYGDEIGMKGGKDPDNRRGMEWNQSKWNHGLRDFTKQLIAIRKSLPMLREGDWTVV
                     QVLSDQQCCVFVRKTQRTFVLILIHNDDHPISIQMDIAPWVGKTARCLEDQLTGQTFK
                     CEQGKVVLSNLGPRSGLILTPVKPAVL"
     gene            complement(5074..>5410)
                     /locus_tag="VLA60_10085"
     CDS             complement(5074..>5410)
                     /locus_tag="VLA60_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09754.1"
                     /translation="TQMPGLNPASGQTAGETSGEIANRVGETLKLGMDLLNSGLGVGL
                     RFLQGLAGPEYHHGHPGWGHTHGAADHFHGFHGCECQMTCGCDCVNCCQSLACASSCG
                     CSPGVGSCC"
BASE COUNT         1480 a         1396 c         1294 g         1240 t
ORIGIN      
        1 cttcgattat atctagaaac ctaatcacag atttactttc ttcaatgctt tcttgcttta
       61 cgccaatcgc tcaactcaat ggtctcatcc tggtttcatg ttcacatttc caagagttct
      121 ggcaaagcga taccccattg atcacagagc aaccggcaaa gcactcaatt ctcaaggata
      181 ttacaagagt cggccaggcc actcccagaa gaatcaccgc ttatcccctc taccgcttta
      241 tttggaaatc tcgataattt caggagtaag gccggtgtcc gtcatatgaa gacgggccac
      301 ggaaatcggg agagtaaatc gacgagggcc ggcactgcca gggttgaggt agagtattcc
      361 cttgcgtctc tcggcagagg cacgatggga atgcccaaaa atgacggcag ataattccga
      421 ggcaaccgga tctatatcca ggtgatccag ttcatgcaca acatagaaaa aatacgattg
      481 atgttcgacg acattcgtca ggggaatctt cgcagcccaa ggatcctgat cattattccc
      541 acgaacggca atcaccggag cgatggcctc caatgtcttc aggacgtcag ggcttccgat
      601 gtctccggca tggataatca attccgcacc ggtcaaagcc gcaatcactt gagggcgcac
      661 caacccgtgc gtgtcagaaa taatcccgat catcttccct ctcttattcc ataccctcat
      721 cctgcgaatc aggattctat tgtcggtcga tcacccgttt tgaaaaattc cccgtaatgg
      781 cgttggccac actccggaca aaacccatgg gtaaattggg cattagatcg atcactaata
      841 aatcgttcga gtggttgcca gttattgttg tcgtcacgaa tctttttgca aaaactgcaa
      901 ataggaagac aaccctccaa tacgttgacc ctattggcta atgctcgtgt ttgcatagcg
      961 gttcgactga caaggaatgc cgccccgcca aggacaagga ttctgatcac cgtgttaatg
     1021 gaggcatcta atatggtgag ggaaaccgac caaaactgta tattaaaaat taatctgata
     1081 agagacaacg tcacggcaaa tgtcagcccc caggaaaatc ccctatacca ggaggcgagc
     1141 gacactggaa taagatacaa aatagggaac tgaatggttg gacctgccag gtaatcacca
     1201 agcaaaatta cgaggaccaa cgcactcaaa tagcccggaa ataatattgg gtgcgtgcag
     1261 gactccttcc aaaagcctgt cctggaggaa aaatttggga aagactgaag aggcggcata
     1321 gcctaaacag aattgagtaa aagaaaaatc ttttgaatta tcatagcata agagtcatat
     1381 gtcgagcatg acgtcctgaa tgattcctgt ccgcccccac ctgtcgggag gaacttgtgt
     1441 gacaattgct gggtctacca cgggcaacca gatcgttcta ctcaggcaac aggaaaagaa
     1501 ctgtccctca tccgggaatc tgaccagcca gtttgtgaac cacattaaaa cttccatggc
     1561 tcatcagtcg gaggaggttt tcctggaaaa agcacttcat tcaggacaca actgtgttcc
     1621 tcaatcccgt ttggggtatc cggacagtca tgtgtggata gcgcttattc ctggaaatgg
     1681 tatagtcatt agtataccgg tcacggttgt aaaggctgcg gcgcattgtc gccgactttt
     1741 tatcaggcaa agaccattaa gaggaatcac gttccattcc tcggttggat tattttctcc
     1801 atggcccccg atgcgaatga cacgtccgga aaggtaagcg agtcagtgaa accgtcacat
     1861 cctgtgttcg accacgccga tgaaatgaaa gagactcccc tcttccagca gcccagtaat
     1921 gggcaaacgc gaccgcccac attatttatc atatttggag cccagggaga tctcacgaag
     1981 agaaaattaa ttcccgcgtt atataacctg gcctcaagtc atcatctccc tcaagaattt
     2041 gccatccttg ggatagacgg gattcccatg catacggatg actttcggga aaagatccgt
     2101 caggacatcg atgaattgtg tccccgcccc atcgatgcca ccatcaaaga atggctcctt
     2161 gaccgcctgc attacctgtc aggggatttc cgggatcctc aaacctacga acgccttgag
     2221 tctctgctga cacaattaaa taccacacat gggacccagg ggaattatct ctattatatg
     2281 gccacggctc caacattttt tgcagaaatc gttgcgcaac ttggcgccaa aggattagtc
     2341 gaacatcgaa aagatttttc ccgtcgtatc gtcattgaaa aacccttcgg tcatgatttg
     2401 ccatcggcac gatcgctcaa tcaagccctg agacaggtgc tcgatgaaag tcaaatttac
     2461 cggatcgatc attatcttgg aaaagaaacc gttcaaaata ttttaatctt ccgctttgcg
     2521 aacggcatct ttgaaccaat ctggaaccgt caatacattg atcatgtcca aatcactgtt
     2581 gcggaaacgt taggggttga gcatcgcggg gcctattatg aaaaagccgg tgcgctccgt
     2641 gacatggtct ccaatcacct cctgcaaatc ttggctttcg tggcaatgga acctccgaat
     2701 acgtttgatc ccgaagcggt caggaacgaa aaagcaaagg tcctccgtgc catccagccc
     2761 atgaaccccg tcgaggtctt acaatcaacg gttgccggac aatatgaggc cggatcgatt
     2821 aatggtcaca gagtacttcc ttatcgaaca gaaaaggatg tcaatccagc ctccctgacg
     2881 gagacctttg ccgcaatgac cttaggcatt gaaagttggc gctgggaagg cgtgcccttt
     2941 taccttcgga ccggcaaacg gttacacacc cgagtgaccg aagtggtcat tcagtttaaa
     3001 tctgccccgc tgatgttatt tcgtaagact cccgtcgacc gactcacgcc gaatgtgctc
     3061 gtcattcgca ttcaaccgga tgaaggcatt tccctaagtt ttggagccaa aattccaggg
     3121 cccaaagtca aggtcgggac ggtggatatg gatttccaat atgcggaata tttcggtgat
     3181 gccccgagca cgggctatga gacgctcctg cacgacgtga tggctggaga tgccacactt
     3241 tttcagcgaa gtgatagcgt cgaggtgggc tggagtatcg tcgatcccat tgtcaaagtg
     3301 tgggagagcc tgggctcgca tgccattcgt ccctatagct cagggagttg gggaccgccg
     3361 gaagccgatg ctttgctggc caaggacggc cgaatgtgga gaaaccacgg gtaaggaaac
     3421 ctttgaatgt ccgcgaatcc caggtgccct tacccaaaaa gatacatttc agaaaaaatc
     3481 ggccctcagc ctcaactttt tcacaggaca gccggtttaa ccggagtcag gatcagtcct
     3541 gagcgtggtc ccaggttgga taagacaacc tttccttgtt cacacttgaa cgtctgtccg
     3601 gtaagctggt cctcaaggca tcgagcggtc tttcctaccc acggggcgat atccatctga
     3661 atagagatgg ggtgatcatc attatgaata agaataagga caaaggttct ttgtgtcttt
     3721 ctcacaaaga cacaacattg ttgatcagag agtacctgaa ccacggtcca atccccttcg
     3781 cgtaacattg gaagagattt ccggatggca ataagctgct tggtgaaatc ccgcaaccca
     3841 tgattccatt tgctctgatt ccattccatg ccccgacgat tgtcggggtc cttccctccc
     3901 ttcatcccga tttcatcccc ataatacacg gccggtatcc ccggaaaaac aaactgaaac
     3961 agcatggcca acttcagccg taaaatattt ccccgggcta aggcatacag gcgttcagtg
     4021 tcatgagacc ctaacacgtt taccatcgct aaggtctttt cccatggata catccgaagg
     4081 aggttgtgca cgcgggacac aaattgcctg gttgaaatgg aaccgtgaat aaagtagtcc
     4141 aagacgacct gacggagtac gtagttcgtt cctccgtcaa attgtccatc ccgaatccaa
     4201 gaacgaccag ggccccagat ttctgccacc atatacgacg acgggtttat ggtcttcacc
     4261 ttctgccaga gcgccttcca aaactgatgg ccccggacat cagccacggc atccaaccgc
     4321 catccatcta taccctgatt cgtccaatag gctgcgacgt ccagaaagta ccgtcgtgtg
     4381 gcaggattgg ctaagttgag aatgggcatg agaggccgtt cctgccaacc ccggtaacga
     4441 aattgcccga atgcattaac cggaaatcgt gtgatgaaaa accaggagat gaagggggat
     4501 gccggaccac gttccatgac atcataaaag ggaaagaatc cccggccgca atgattgaat
     4561 acgccatcca acaatatgcg aatgccattc gcgtgagccc tggataagag agtctgaaag
     4621 tcagccaatg tcccaaggcg aggatcaatc ctatagtaat cgtaggtgtt gtatcgatga
     4681 ttggccgagg caaaaaaaat gggggccagg taaatcagat tgacaccaag gtccaggaga
     4741 tactcgagat gttcagtgat ccctcgcaga gtacctccca taaatccatg agctgtgggc
     4801 ttggatcccc aggatacgaa accggatcca ggcttgagct gttgggtatc tcgcgcaaac
     4861 cggtcaggaa agatctgata gattatggca tcacgagtcc aggaaatgtc ggaagcgggt
     4921 tgatgacgtc tcatagatat acaatcagaa actcgttcac gggtaggacg tggatattgg
     4981 tgtgcaaaga aaaagaacga aacccggagt cacaagacac tccgggctag aggaagagga
     5041 agaagtccgc tggcaatctt tcaaaactgc cggttaacaa cacgagccta cgccgggaga
     5101 acacccacag gaggatgcac acgccaggga ctgacaacaa tttacgcaat cgcagccaca
     5161 ggtcatttgg cactcacatc cgtggaatcc atgaaaatgg tcggcggccc cgtgggtatg
     5221 gccccatcca gggtgtccgt gatgatattc agggccagct agcccttgaa gaaaccgtaa
     5281 tccgaccccc aacccagaat tcagaagatc cattcccaac ttcagggttt cccctacccg
     5341 gttagcgatc tccccacttg tctcgccggc agtttggcct gaggcaggat tcaaaccagg
     5401 catctgggtt
//
LOCUS       DATIAL010000324         6651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_168049, whole genome shotgun sequence.
ACCESSION   DATIAL010000324 DATIAL010000000
VERSION     DATIAL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168049"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1465
                     /locus_tag="VLA60_10090"
     CDS             47..1465
                     /locus_tag="VLA60_10090"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HSF09755.1"
                     /translation="MLKKVLVANRGEIAMRVIRACREFGIATAAIYSESDATAIYVKK
                     ANEAYLVGPGPVQGYLDARQIVGLAKRIRADAIHPGYGFLAENADFARLCEEAGILFI
                     GPSPHSLELLGDKVQARALAIKIGVPVVPGTDGSVSTLEEALTFSHKAGYPVMVKASA
                     GGGGRGLRVVRSDDELQDAMEAGAREALAAFGNGELFLEKYVERPHHIEFQLLADKNG
                     YVIHLGERDCSIQRRHQKLIEIAPSLVLTPRLRAEMGEAAIAMARAAQFYNAGTVEFL
                     LDPDGRYYFMEMNPRIQVEHTVTEQITTVDIVQSQLWIAAGRPLVFGQHEVNLQGYAI
                     QCRVNAEDPQNGFRPSSGKITAYLSPGGVGVRIDGAVYKDYTVPPYYDALLAKLTVHG
                     RTWDETVRRTHRSLEEFVLRGVKTTIPFMTKLMEEPDFRAGRFDTSYLERHPELFEYE
                     EAEEPEDLVIALSAAIAAFEGL"
     gene            1517..3457
                     /gene="oadA"
                     /locus_tag="VLA60_10095"
     CDS             1517..3457
                     /gene="oadA"
                     /locus_tag="VLA60_10095"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HSF09756.1"
                     /translation="MSRKTAHSDKSRKPSRPVPTRTKLDAAPALDIEASPKRQVFLTD
                     VALRDGHQSLLATRMRTEDLLSVASELDEVGFWSLEVWGGATFDSCLRFLKEDPWERL
                     RAFREAMPKTRLQMLLRGQNLVGYRHYADDVLEKFIELSAQNGIDVFRIFDALNDIRN
                     IRPAMEVVKACGKHIEATICYTVSPVHSLNHFVEQAKQLEDLGTDTICIKDMAGLLPP
                     FEAYELISRLKATVRVPIHLHTHYTSGMASMSSLMAIMGGLDILDTALSPLSGGTSHP
                     PTESFIAALRNTPYDTKLNLEQIAPAADKLRKARKRYRQFESDFTGVDTDILLSQVPG
                     GMLSNLAAQLAEQNALGKIKEVLEEIPRVRKDMGYPPLVTPTSQIVGTQATLNILTGE
                     RYKVITNETKNYFQGLYGKPPGTLNSKIRQKALEGDVPVSGRPADNLDPELDDAKQEL
                     VGREMAEEDIVSYALLPSVALQFFDERERGELKPEPLHDSSESGPAVAHDLHLAPVEF
                     NVTVHGEAYHIRVSGSGQTVDGVKPYYIKVNDKLEEVYLEPIQEVLAGSPDPPPSQTS
                     KREKRPKPSQPGDVTTPMPGRVVKVLVTEGSSVNVGDSLLVVEAMKMENRVQSPIAGS
                     VITIYVKEGDEVNPDETLIHLE"
     gene            3526..4413
                     /locus_tag="VLA60_10100"
     CDS             3526..4413
                     /locus_tag="VLA60_10100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF09757.1"
                     /translation="MGCSSLRALPSGIELIQRVPYQVTRVNNHRIAYLDVGQGPPLIL
                     IHGFGGSIWQWEHQYSVLAHTHRVIILDLLGSGLSDKPEDAYTPKYLLEFFREFMDTL
                     NIPRATLVGNSMGAGLAMAMALDYPERVERLVLISGFPAQVENSIASPHYQSFLYHRP
                     PLWLAKLGNWMAGKRTTEHILKEIIYNSALISPTIVDRSFHNRQRGDFLAPLYSLMDN
                     IKTWEGQYGNRLQKISHQVLLLWGEHDRVFPLEVGERVKDQLPHVEWHVIPEAGHLAQ
                     WEAPTIVNQYILSFLERKS"
     gene            4526..5179
                     /gene="tpx"
                     /locus_tag="VLA60_10105"
     CDS             4526..5179
                     /gene="tpx"
                     /locus_tag="VLA60_10105"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HSF09758.1"
                     /translation="MPKISNISFILILTASALTGCSTLNGQSAHDFSYKNIPVDKETA
                     RTGEGSTILFRGTPLPLSGIEVKAGETLRAVPLAKGDLSLVNIHESQGKVRIINIVPS
                     LDTKVCEQQTHYLSEKNQGLDQQVQLITISVDTPFAQKRFAKEAGITNVEFLSDFRGG
                     EFGSSHGLLLEGPHVLARAVMVVDANNVIRYLQVTPDLGHMPDMDRAFQVARSVLQN"
     gene            5220..5888
                     /gene="tenA"
                     /locus_tag="VLA60_10110"
     CDS             5220..5888
                     /gene="tenA"
                     /locus_tag="VLA60_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HSF09759.1"
                     /translation="MTFSEQLRNRAAPIWAAQLQHPFVVALGKGTLPAKKFQYYILQD
                     ARYLEELARVFALGSAKATDGDTALRFAKLVEDTITVERGLHESYGKQWALSPKDMRS
                     TPLSPTNYAYCRHMRSVAQTGTLGEITVVALPCAWVYCEVGHHLLKKATPKFSHPYYE
                     WLQLYGSPEFAEVTRWLREVVDRCAKTAGRAEKARMEEAFLISSQYEWMFWDMAWREE
                     KWPI"
     gene            5996..>6651
                     /locus_tag="VLA60_10115"
     CDS             5996..>6651
                     /locus_tag="VLA60_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09760.1"
                     /translation="MKRSPTALLYSLALFNLWIGLQFPDQVSGQPPMAKGIQPLIQLE
                     SQSLHQLIPKNIRPLLLPKEQEQFLQELEGQPPDWHTLQSLDHTEQSERLFQLNRARD
                     ETRLIHKDILQQPIAFLWSGLLRRYMPEYQGFLVALGPELTSTSWGIVRFKPMGLPDY
                     LVAIPSLESAKQIQSQQQKGEQIAIGVLFFGTLVADESLIYSFSHDQKGDGMILPVVQ
                     "
BASE COUNT         1719 a         1741 c         1585 g         1606 t
ORIGIN      
        1 ccccagtcat ttatttcaat ttattacgac acgacactag aatcgaatgt taaaaaaagt
       61 tttagttgcc aatcgaggag aaatcgcgat gcgggtcatc cgggcatgtc gagaattcgg
      121 cattgccacg gcggcgattt attcagaaag tgacgccacc gccatttacg tcaaaaaagc
      181 caatgaagcg tacttagtcg gtcccggccc tgtacaaggc taccttgatg ctcgacaaat
      241 tgtaggattg gcgaagcgca ttcgagccga tgccattcac ccgggatatg gttttttagc
      301 agaaaatgcc gattttgcgc ggctctgcga agaagccggc attctcttta ttggcccatc
      361 tcctcattca cttgaactac tgggcgataa ggtccaggct cgagcactgg ccattaaaat
      421 tggcgtccct gttgttccag ggacagatgg cagtgtgagc accctcgaag aggcgctgac
      481 cttctcccat aaggcgggat acccggttat ggtcaaagcc agcgccgggg gtggagggcg
      541 aggcctccgc gtcgtacgct cggacgatga attacaagat gccatggaag cgggagcccg
      601 ggaagctctg gcagcctttg gaaacgggga attatttctt gaaaaatatg tggaacgacc
      661 ccaccatatt gaatttcaat tactggcaga caaaaacggt tatgtgattc atctgggaga
      721 acgggattgt tcgattcagc ggcgccatca aaaattgata gaaatcgccc cgtcacttgt
      781 gttaacccct cgactacggg cagaaatggg agaagcggcc atcgccatgg ctcgcgccgc
      841 ccaattctat aatgccggga ccgtggagtt tctgttagat cccgacggac gatactattt
      901 catggaaatg aacccccgca ttcaagtgga acataccgtt acggaacaga tcaccaccgt
      961 tgatattgtg caatcccaac tatggattgc cgcagggcgt cctctggtct tcgggcagca
     1021 tgaggtcaat ctccagggct atgccattca atgtcgggtg aatgcggaag atccccaaaa
     1081 cggatttcga ccctcttcgg gaaaaatcac ggcctattta tctcctggcg gtgttggagt
     1141 tcgcattgac ggtgcggtgt acaaggatta taccgttcct ccgtattatg atgccctgtt
     1201 agccaagttg acggttcatg gccgaacctg ggacgaaacg gttcgccgaa ctcatcgctc
     1261 actcgaagaa tttgtcctgc ggggtgtcaa aaccactatt ccatttatga caaagctcat
     1321 ggaagaaccg gattttcgag ccgggcgatt tgatacctcc tatcttgaac gccatccaga
     1381 actctttgaa tacgaggaag cagaagaacc ggaggatctg gttattgccc tgtccgcagc
     1441 catcgccgca tttgaaggat tataacaacc gtcatgataa cgttgtgatc ccgttgcaca
     1501 tgtgaaaggt cgccttatgt cacgaaaaac cgcgcactct gataaaagca gaaaacccag
     1561 tcgtcccgtc cctacccgga caaaattgga tgccgctccc gctctggata tcgaagcatc
     1621 tccgaagcgc caggtctttt taaccgatgt ggctctacga gatggacacc aatctctttt
     1681 ggcgacccgc atgcgcacag aggatctctt atccgtggca tccgaattag acgaagtcgg
     1741 attttggtca ttagaagtat ggggtggtgc caccttcgat tcatgcctcc ggtttttgaa
     1801 ggaagacccc tgggagcgcc tgcgagcctt ccgggaggcc atgccgaaaa cccgtcttca
     1861 aatgctcttg cgtgggcaaa accttgtggg atatcgacac tatgctgacg acgtccttga
     1921 aaaattcatc gaattgtcgg ctcaaaacgg cattgatgtc tttaggatct ttgatgcgct
     1981 gaatgacatc agaaatatca ggccggccat ggaagtggtg aaagcttgtg gcaagcatat
     2041 cgaagcaacc atttgttata ccgtcagtcc ggtccacagt ctgaatcatt ttgtggaaca
     2101 agccaaacaa ctcgaagatc ttgggacaga caccatttgc atcaaagata tggccggact
     2161 ccttcctccc tttgaagcct atgaactgat ttcacgatta aaggccacgg ttcgagtccc
     2221 gattcacctg cacacacatt acacctccgg aatggcttcc atgtcttccc tgatggcgat
     2281 tatgggcggg ttggatattc tggataccgc attatctccc ctttccgggg gtacctccca
     2341 tcctccaacc gaatccttta ttgccgccct gcggaacaca ccgtatgaca cgaaattgaa
     2401 tcttgagcaa attgctccgg cggcggataa attacgcaag gcccgaaaac gctatcgcca
     2461 atttgaaagc gactttaccg gtgtcgacac ggacattctt ctctcccaag tgcctggcgg
     2521 aatgctctcg aatttggcag cccaattggc cgagcaaaat gcgctgggga aaatcaaaga
     2581 agtgctggaa gaaatcccgc gcgtgcggaa agacatgggg tatcctcctc tcgtcacccc
     2641 caccagccaa attgtcggaa cgcaagcgac gctgaatatt ttaaccggcg aacgctataa
     2701 agtgattacc aatgaaacca aaaattattt tcagggcttg tatgggaaac cacctggcac
     2761 cctcaactcc aagattcggc aaaaagcctt ggaaggcgac gtccccgtgt ccggtcgtcc
     2821 cgctgacaac ctggaccctg aactggacga tgcaaaacag gaactcgtcg ggcgagagat
     2881 ggctgaagaa gacattgtct cctatgcact cctcccctca gtagcgttgc aattttttga
     2941 tgaacgggag cgaggggagc taaaacccga acccctacat gactcatcgg agagtggccc
     3001 ggctgtggct cacgatcttc atttagcccc ggtagaattt aacgtgacgg ttcacggcga
     3061 agcctatcac attcgcgtat cgggatccgg gcagacagtt gacggcgtga aaccatatta
     3121 tattaaggtg aatgacaaac ttgaagaagt gtatctcgaa ccgattcaag aagtcttagc
     3181 cggctcacct gaccctccac catcccagac atcaaagagg gaaaaacgtc ccaagccatc
     3241 acaacctgga gacgtgacca cacccatgcc tggccgggtg gtcaaggtcc tcgtaaccga
     3301 gggttcaagt gtcaacgtcg gtgattccct gttggtggtg gaagccatga aaatggagaa
     3361 tcgcgtccaa tcgcccattg ccgggtccgt catcaccatc tacgtgaaag aaggagatga
     3421 agtgaatcct gatgaaacgt tgattcatct ggaataataa tgcagggctg ttctcacttt
     3481 cgatcattcc tccttttcat ggtctcgctt ggatgcttga cgatcttggg ttgttcctct
     3541 ctcagggcac ttccttccgg aatcgaactg atccaacgtg tgccctatca ggtcaccaga
     3601 gtcaacaacc atcgcattgc ctatcttgac gtcggacaag gtccaccact tatccttatc
     3661 catggatttg gcgggtcgat ttggcagtgg gaacatcaat attctgttct ggcccatacg
     3721 catcgggtca tcatcctgga tttactcggc tctgggctct ctgataagcc ggaagacgcg
     3781 tacacaccaa aatacctctt ggagtttttt cgggaattca tggataccct caatattcca
     3841 cgtgccacct tagtcgggaa ttccatggga gccgggttgg cgatggccat ggcccttgac
     3901 tatccggaac gcgtggaacg acttgtgctc atctcgggat ttccggccca ggttgaaaac
     3961 agtatcgctt ccccccatta ccaaagcttt ctctatcacc gcccccccct ctggctcgca
     4021 aaattgggaa attggatggc aggcaaaagg accacggagc atatactcaa agaaatcatt
     4081 tataattcag ccctcatctc ccctactatc gttgaccgtt catttcacaa tcgccaacgg
     4141 ggcgattttc ttgcccccct ctattcttta atggacaata tcaagacttg ggagggacaa
     4201 tatggaaata ggcttcaaaa gatttctcat caggtcctgc tcttgtgggg ggagcatgat
     4261 cgggtgtttc cactcgaagt cggtgaacgg gtaaaagacc aattaccgca tgttgaatgg
     4321 catgtcatcc ctgaggccgg gcaccttgca caatgggaag cacccactat cgtgaatcaa
     4381 tacatcctct catttttaga acgaaaatcc tgaagaaacc attatccaac agactactca
     4441 gcctacactt gaaagaaaat gatatttttt acacaccacg gcacctcaag ctcggatcac
     4501 cgatccggat agaaggagca tcactatgcc aaaaatttct aatatttcgt tcattctcat
     4561 cttaacggct tctgcattga cgggttgttc gacccttaat ggtcaatcag cacacgattt
     4621 ttcctataaa aacattccag ttgataagga aaccgccaga acaggagagg gttcaaccat
     4681 tctatttcga ggcaccccct tgccgctgag tggcattgaa gtgaaagcag gagagactct
     4741 tcgtgcggtc ccattggcca agggtgattt atcactcgtc aacattcatg aatcccaagg
     4801 gaaagtccgc atcatcaata ttgtgccttc cctggacaca aaagtatgcg aacagcaaac
     4861 gcattatctg agtgaaaaaa accagggcct tgatcaacag gtgcaactga taaccattag
     4921 tgtcgatacc cctttcgctc aaaaacggtt tgcgaaagaa gcgggcatta ccaatgttga
     4981 atttctgtcc gattttcgag gcggagaatt tggttcttcc catggactcc tgttggaggg
     5041 gccccatgtc ctggctcgag ccgtcatggt tgtcgatgcg aataatgtaa tccgctatct
     5101 ccaggtcact cctgatctcg gccatatgcc ggatatggat agagcgtttc aagtggctcg
     5161 ctccgttcta caaaactagt ggcaccactc acatccagaa aaattcacta cggactttca
     5221 tgacgttctc agaacaatta cgaaatcgtg ccgcgcccat ttgggcagcc cagttacaac
     5281 atcccttcgt cgtagcattg ggcaaaggca cgttaccggc aaaaaagttt caatattaca
     5341 tcctacaaga tgcaagatat ttagaagaat tggccagagt ctttgctcta gggtcggcca
     5401 aagcgacgga tggagatacg gctcttcgct ttgccaaact cgttgaagac acgataaccg
     5461 tcgaacgagg gcttcatgaa tcgtatggaa aacaatgggc tctttccccc aaggacatgc
     5521 gctccacccc cctttctcca acgaattatg cctattgccg gcatatgaga agcgtggctc
     5581 aaaccgggac actcggggaa attacggtgg tggccctccc ctgtgcatgg gtctattgtg
     5641 aagtcggaca ccaccttttg aaaaaggcaa cccccaaatt ctcacatccg tattatgaat
     5701 ggttgcaact ctatgggtca cccgaatttg cagaagtcac ccgatggctg cgcgaagtcg
     5761 ttgatcgctg cgctaagacc gcaggcaggg cagaaaaagc caggatggag gaagcctttc
     5821 tcatcagttc gcaatacgaa tggatgtttt gggatatggc ctggcgggaa gaaaaatggc
     5881 ctatatagcc gatggggtgt gtttatcttt ttataccatg acgtgagctg aaatcactca
     5941 ttacaaaact ctaagctttc tttcacccag caccagtctt cgagaacgca aacacatgaa
     6001 aaggagccca accgccttat tgtattctct cgcgttattc aacttatgga tagggcttca
     6061 gtttccggat caggtcagcg gacaaccccc catggccaaa ggaatccaac cccttatcca
     6121 actcgaaagc cagtccttgc accaactgat ccctaagaat atacggcccc tcctgcttcc
     6181 aaaagaacag gaacaatttc ttcaggaact tgaaggccag ccgccggatt ggcacacctt
     6241 acagagcctg gatcacacgg agcaaagcga gcgcctgttc caactgaatc gcgcacgaga
     6301 cgagacccgt ctcattcaca aggacatcct tcaacaaccc attgccttct tgtggtcagg
     6361 acttctccgt agatatatgc ccgaatacca gggatttttg gtggcccttg gcccagaact
     6421 cacatccaca tcatggggta ttgttcgctt taagcccatg ggattgcccg actatctggt
     6481 ggccattccc tctcttgaat cggccaagca aattcagagt cagcaacaaa agggtgagca
     6541 aattgccatc ggcgtccttt tttttggtac tctggttgcc gatgaatccc tcatctatag
     6601 tttttctcat gatcagaaag gggacggaat gatcttgccc gtcgtgcaaa t
//
LOCUS       DATIAL010000325         9539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_813176, whole genome shotgun sequence.
ACCESSION   DATIAL010000325 DATIAL010000000
VERSION     DATIAL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9539
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813176"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1092)
                     /locus_tag="VLA60_10120"
     CDS             complement(124..1092)
                     /locus_tag="VLA60_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF09761.1"
                     /translation="MTWLKLVEGYMPMQMISELAVCILVFSIINYSLKRAGMGLPKFW
                     AGIFVWCFVNLFYLKYRIYPPIPFSVRAIYGTVSACGIFMWVSGSQQEWEEFKRPIIN
                     VLDAKTGMTKFIRTALLILLPIGLWGFAYNSFLPSFDEPIELRTVHPAPPATTKVHGK
                     TYVLQTAANPYRINPEGKYDQAYSNAHIVSQDMGRLMKDVKNPEDNPWDPNAKGYIKH
                     VREGGEIFFQNCHFCHGDNLNGRGLWAFAFNPIPANFTDAGTIAQLQETFVFWRVAKG
                     GIGLPGEGFPWASVMPPWEQHLTVDEIWKVIMFEYWHTGYYPRTWE"
     gene            complement(1138..2451)
                     /locus_tag="VLA60_10125"
     CDS             complement(1138..2451)
                     /locus_tag="VLA60_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase"
                     /protein_id="HSF09762.1"
                     /translation="MVELLGRAIGMGWPVLLMLVGLLLYFQATISDPVKKKRASFQTI
                     VGIFCAFLAFIAISNYTHNFEGESRLLPVSLVMITIMAFVMGLYFPNISALMKIGGFM
                     FFVAAALSGYGNWLPQVEGGFPPPVVKLDFQSMSAQQLGDEGEKIIFGGIGQSKTQGA
                     IGKGQCPLCHGFQQGFLSERAPNLYGIPDTAPQRLKEPNYHMNNPEARTTEQKEAFPG
                     SGTATNAQEYIAESHACPSCFVVTGFGVKGSNDTVSPMPKIHKPPISLTLGELAAVDT
                     WIYTREGKEPPPYEEIVASYEKFIPEADRPSAGGEEEAGAGGGNLLADGSEPYDKLFM
                     KAGCPACHTIPGIEGATGKVGPLLMEGSNAPNRLKDPAYQGKAKSPKEYITESILNPS
                     AYVVKDFPDNQMPKDFGVRLTGGALNKMVDYLAQLKEGQPLPPKE"
     gene            complement(2519..4489)
                     /locus_tag="VLA60_10130"
     CDS             complement(2519..4489)
                     /locus_tag="VLA60_10130"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSF09763.1"
                     /translation="MGRLFNQMTRGKKKLFAVSALMVMGCLLLLPIFLSVTAVAGGGG
                     APAGGPPADVVAAQKAAGEEGEAEKVEMGRDVYYKTEGPAIGMPAPVTEDNETFYPRY
                     NFESRVLLWVANQQHLYYGSFVLAVPIFCMCIEFAGMVSKDKAMAKKYDQLAYDFIKI
                     SLTAYSLTAILGGILIFTFLTLYPAFFGYLSSIFRPVMHIYALTFVAESATLYIYYYG
                     WDKMREGVLKWVHLSMSVILNVIGTVLMFLANSWIAFMMSPAGVDEQGRYLGNIWHVI
                     HTALWNPLNVHRILGNMAFGGGVVAAYAAYRFLSSKTDEERAHYDWMGYIAMSLGVAF
                     LIPLPFAGYWLMREVYAYRQQMGITLMGGLLAWLFIIQATMIGILFLTTNYYLWQALG
                     RMTGGERFQKYIKYLVFILVVGLLVFITPHTIVMTPAELKAMGGQQHPVLGNYGVMSA
                     KNGGINAIIMTTVLSFIWYQRGNRVPTVSWAKFGNIFMGCFFIIAQLNNVWLACYGYF
                     IPANVRIGLSVPQVAGTLSCLLLMTPLNLAMLKNGRQLGPIRWGQIPPRSQYAIIMLA
                     TAFTWMMGLMGYIRSSVRLFWHVNEVMRDNSPWAYTHTIGFAANVISFNVLFFWISIM
                     FVFWLGTLGAKKVPVPSPAGQPVPSPQSATGH"
     gene            complement(5178..6062)
                     /locus_tag="VLA60_10135"
     CDS             complement(5178..6062)
                     /locus_tag="VLA60_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF09764.1"
                     /translation="MDGRFKLIFLIAVLFMTGLPVLGILRGTDAPPTPPDRDLQSHPS
                     LASETSPEALKDEALSGRANEDEMVEIPAGEFILGSDKGGFNEKPAHVAHLDAYWIDR
                     YEVTYQRYMEFVEATGHRQPGPPSRYAEKLGLLRGPHQPITYVSWSDANDYCQWRGKR
                     LPTEQEWEKAMRGTDGRTWPWGEGLSGHPANFAGEADGYVVSAPVGAFPLDKSVFGVY
                     DGAGNVMEWTDNWYVEDLYLQENAATPTSNRSPSTYKTMRGSGYTSQGVDIRITNRSF
                     MVPDFRDETIGFRCARSD"
     gene            complement(6148..7065)
                     /locus_tag="VLA60_10140"
     CDS             complement(6148..7065)
                     /locus_tag="VLA60_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF09765.1"
                     /translation="MENQRVLMGAILFVFGSFIMMIVMLIVMTYKGKKDLDELSAGQS
                     ANVRVLQPMPTQDFSMYKTLVGDDNREMVEIPEGPFTMGIGDGDPDEGPPHPVYLQTF
                     YIDLKEVTQADYERYINMTKRDKPKVPVFEDDVSKLIGPDYPVVAVTWNDAFGYCRWA
                     GKRLPTEAEWEKAARGEGKRRYPWGDKFDYQFANVDGEEDGFKFLAPVGSYEVGRSPF
                     GLYDVTGNVAEWVMDSYAPDYYQQAPYRDPPGPKDEDENKVIRGGSWRESRIGARVTK
                     RFAAKMWRNDASVGFRCAKDPPNENPQSS"
     gene            complement(7116..7493)
                     /locus_tag="VLA60_10145"
     CDS             complement(7116..7493)
                     /locus_tag="VLA60_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09766.1"
                     /translation="MKVTPSKIFDVLLGILGLGTVGLLIGIFMGEGWLPVALTAGALL
                     GAGVGLVGGRGFFLSIFIGTILGGLLALGLSGTEAVTVGAASGAAMGGFLGIWISMLI
                     ETWQQRNQNLPESNVKHHGPMQP"
     gene            complement(7497..8231)
                     /locus_tag="VLA60_10150"
     CDS             complement(7497..8231)
                     /locus_tag="VLA60_10150"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSF09767.1"
                     /translation="MRICSWQHNNLEGVKRSRVSAACLAWLIVGLLINGSSAAWAQEV
                     PSSGPSGSSMVLEDFQHPDAKGFPQGWEAQRSTVTAHETYTIQEEGGAFFLSAKNANQ
                     RVYTKQMTWDPKQHPILTWRWRLQSVPDNADFLAAIYPSLDVDLMFIPVNTKYVWSAT
                     LPVGSVKEGGMFSSTEIVIRSGTESLGKWVEERVNVYEDFLKIHDHEPAPHAWGISLL
                     GGPGVEVDFGSIRVQSESSSVDLGRE"
     gene            complement(8231..>9539)
                     /locus_tag="VLA60_10155"
     CDS             complement(8231..>9539)
                     /locus_tag="VLA60_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09768.1"
                     /translation="ALKWVHMTLGVLSNATGVILLLLANSWASFMMAPAGVDAQGHFL
                     GNVWHLLHSPLWNPLNTHRFLADIMSGGAVVVAYATYRFFMSKTAEDRAYYDWVGHVF
                     LVVVVCALLPMPLAGYWLMRAVFEFRQTMGMAMMGGMLSWLFVLQAIMVGVLFLGINY
                     YIWQSLARLQGGERFHPHFKAILFGLMVCFLVWFTPHTIAMSGSEMKAMGAAQHPVIG
                     QFGVMSAKNGAVNVMICLTTLSYILYRRANRVMTVKWASKGNIFLLMLFLMGIANIVW
                     LAIYGFYIPANVRVGLSSPQGMTTATVVVGGILLNRLMLRGAHINGPVHWGRITPRGI
                     VALFVVAAAFTWVMGLMGYIRSVGRLGWHISELMPDRSSWAFTPSLGFAAKMVTLNMI
                     VFWSSVFFVFWLSRWDQRVVERRTAEFARPSPVIQVISEEESA"
BASE COUNT         2644 a         2628 c         1944 g         2323 t
ORIGIN      
        1 caactcagaa gccaggctcc taggccacgc ctaccatatc cggctgtagt cgtttcgttc
       61 atgaccatcc tcttttcaaa gtgtccagta tcgaattgaa ttccattcat gtccgcgtcc
      121 tccttattcc caagtccgcg ggtaataccc ggtgtgccag tattcgaaca tgatgacttt
      181 ccaaatttca tctaccgtta aatgctgttc ccacggtggc atcacagatg cccatgggaa
      241 accttcacct ggaagcccaa ttccgccttt agccacacgc cagaaaacaa aggtttcttg
      301 gagctgtgcg atggttcctg catccgtaaa attggcagga attggattaa aggcaaaggc
      361 ccagaggccc cggccgttca aattatcccc atgacagaaa tggcaatttt ggaaaaagat
      421 ctctccccct tcccgaacat gcttaatata acctttagca ttcggatccc aggggttatc
      481 ttccgggttt ttcacatctt tcattaaacg tcccatatcc tgacttacga tatgagcgtt
      541 gctgtatgcc tgatcgtatt ttccctctgg attaatacga tagggattcg cggcagtttg
      601 caatacatac gttttcccat gcacctttgt cgtcgccggt ggggcaggat gcacggttct
      661 caattcaata ggttcatcaa agctgggaag gaaagaattg taggcaaacc cccaaagccc
      721 gattggcaat aaaatcaata aggctgttcg aatgaactta gtcattccag ttttggcgtc
      781 cagaacattt atgataggac gcttaaactc ctcccactcc tgttgtgagc cagacaccca
      841 cataaaaata ccgcaagcgg aaaccgtccc ataaatggcc ctcacagaaa agggaatggg
      901 cggatagatt cgatacttca gatagaacaa attcacaaaa caccagacaa agattcccgc
      961 ccaaaacttt ggcaaaccca tgccggctct tttaagagaa tagttaataa tagaaaacac
     1021 aaggatgcag accgccaact ctgaaatcat ctgcatcggc atgtagcctt caactagttt
     1081 aagccacgtc attcttcctt actccttata aagggttatc ccctaaatta atattcctta
     1141 ttcttttgga ggtaaaggtt ggccttcttt taattgcgcc aagtaatcaa ccattttatt
     1201 gagagccccg cctgtcaatc ttacgccaaa atcctttggc atttgattgt ccggaaaatc
     1261 cttcacgaca taggcacttg ggtttaatat cgactcagta atgtactcct tgggcgattt
     1321 ggctttcccc tgataggctg ggtctttaag gcgatttggg gcatttgacc cctccatgag
     1381 gagaggaccc acttttcccg tggcaccttc aatccctgga atcgtatgac aggccggaca
     1441 gccggctttc atgaagagct tgtcgtacgg ttcgcttccg tcagctaaaa gatttccacc
     1501 acctgcccca gcttcttcct ccccacctgc ggatgggcga tcggcttccg gaataaactt
     1561 ttcatatgaa gcgacgattt cctcatatgg cggaggttct ttaccttccc gcgtgtatat
     1621 ccaagtatca acagcggcca attcccccaa agtcaaagat atcggtggtt tgtgaatctt
     1681 cggcatagga cttaccgtgt cgttcgaacc cttcacacca aaaccagtca ccacaaaaca
     1741 acttggacat gcatgtgact cggcgatata ttcttgggca tttgttgccg tgccggaccc
     1801 tggaaaagct tccttttgtt cagtggtgcg tgcctcagga ttattcatgt gataattcgg
     1861 ttcttttaat ctctgaggcg ccgtatccgg aattccatag agattcgggg cccgctcact
     1921 caaaaatccc tgttgaaatc catggcataa tgggcattgg cctttgccga tggccccttg
     1981 tgttttgctt tggccaattc caccaaaaat gattttctct ccctcatcac caagctgttg
     2041 cgctgacatg ctctggaaat ctaacttcac gacaggagga ggaaatcctc cttcgacttg
     2101 aggcagccaa tttccgtagc cggagagcgc ggcagctaca aaaaacatga agcctcctat
     2161 tttcatcagc gcactaatat tgggaaagta gaggcccatc acaaatgcca tgatcgtgat
     2221 catgaccagt gaaaccggca acaaacgact ttctccttcg aagttatgcg tataattgga
     2281 aattgcgata aaggccaaga atgcgcagaa aattccaact atcgtctgga aacttgctct
     2341 tttctttttt accggatcag aaattgttgc ctgaaaatac agcaacaacc ccaccagcat
     2401 cagcaatact ggccaaccca tccctattgc ccgtcccaat aactcaacca cagtgcaatc
     2461 ctccaacttg ggggctgcct attgcagccc cacgattgtt tgtaattgta tttttcgttc
     2521 aatgaccagt tgcagactga ggagatggaa ctggctgccc tgcgggcgaa ggaaccggta
     2581 cttttttcgc accaagggtt cctaaccaaa agacaaacat aatactgatc cagaaaaata
     2641 acacgttaaa cgaaataacg ttggccgcaa acccgattgt atgggtataa gcccagggag
     2701 agttatcccg catgacctcg ttgacgtgcc agaacaatct cacggaagaa cggatgtagc
     2761 ccatcaatcc catcatccat gtgaatgctg tggccaacat gatgatggca tattgggaac
     2821 gtgggggaat ttgaccccat ctaatcggac ccagctgtct tccatttttc aacatcgcaa
     2881 gattcagtgg ggtcattaac agtaggcatg acaatgttcc ggccacctga ggcaccgaca
     2941 atccaattcg cacgttggca ggaatgaaat acccatagca agccaaccaa acattgttta
     3001 gctgggctat gatgaaaaaa catcccataa aaatattgcc aaatttggcc cagctaaccg
     3061 ttgggaccct gtttcctcgc tggtaccata taaagcttaa aacggtcgtc atgataatgg
     3121 cgttaatgcc gccatttttt gccgacatga caccataatt acctagaacc ggatgttgtt
     3181 gaccacccat agctttcaat tcagccggag tcatgacgat cgtatgtggc gtaatgaaaa
     3241 ccaaaagacc aacaactaga ataaacacga gatattttat atatttctgg aatcgttcac
     3301 cacccgtcat tcgtccgaga gcctgccaca agtaataatt tgtggtcagg aacaagattc
     3361 cgatcatggt ggcttgaata ataaacaacc aagctaacag accacccatc aaggtgatac
     3421 ccatttgttg tcgataggca tacacctcac gcatcaacca atatcctgca aagggcaacg
     3481 ggatgagaaa tgccacacct aaactcattg cgatgtagcc catccagtca taatgagccc
     3541 gctcttcatc tgtttttgat gaaaggaagc gataggctgc ataggctgcc accacaccac
     3601 caccaaaggc catgttcccg agaattctat ggacattcaa aggattccac agcgccgtat
     3661 gaattacgtg ccaaatattt ccaagataac gtccttgctc atctacccca gcgggtgaca
     3721 tcataaatgc aatccacgaa ttggcgagga acatcagcac cgttccaatc acattcagga
     3781 tgacggacat gctgagatga acccatttca gaacgccttc ccgcatcttg tcccaaccgt
     3841 aataataaat gtagagggtt gcactttccg ccacgaacgt taaggcatag atgtgcatga
     3901 ccggccggaa aatactggat aagtacccaa agaaggccgg atagagcgtg aggaaggtaa
     3961 aaatcaaaat cccaccaaga atggcggtta aggaataggc cgtcaggctg atcttgataa
     4021 aatcataagc caattgatca tattttttgg ccatggcttt atctttgctg accatcccgg
     4081 caaattcgat gcacatgcaa aagattggta ccgccaacac aaaacttcca taataaagat
     4141 gctgctggtt ggccacccaa agaaggacac gactttcaaa gttataccga gggtaaaaag
     4201 tttcattgtc ttccgttacc ggtgccggca tcccaatggc tggaccttcg gtcttgtaat
     4261 atacgtcccg gcccatttcc accttttccg cctcgccctc ctcacctgct gccttttgcg
     4321 cggcaacgac atccgcagga ggacctccag ccggtgcacc acccccacct gccacggcgg
     4381 tgactgacag gaaaatcggc aacaacagca gacagcccat caccatcaag gccgatacgg
     4441 caaacaactt tttcttcccc cgggtcattt gattgaacag gcgacccatt gacctacctc
     4501 cttgtgaaaa cgtgttgagt taaacccatc aaaattacct aattaatcgt cttagtctta
     4561 agttccaagc tcatagattt ttcaactatc ggaacatgta gaaatagtcg agtccctggg
     4621 catccatcaa gatataatcc aatgcctgga aatatacaaa acaaatgacc aatgaaacta
     4681 tgacatacag agctgtctgc atgcgctcct ccttgggtct aaaagaatta aatgattgat
     4741 taccatattg gaagtcatga tttttagcag ggacactgaa taccggcaaa ccaataaaag
     4801 aaccacaagc ttaccgcgca tgtcagataa aaaatcgctt tgcctatttt ggtctctaac
     4861 tccgttgact cccttatgac agccgtcgaa tcccccatct taattcctcc ctatgcttaa
     4921 ttcgcatcca aaaaattaaa cgatttcacc taataaactt cattgacaca ttattatttt
     4981 tcgtgctatt caaacattta ttgatcacgt aaaacgaggg attactcatg cgtacaaatg
     5041 caaaagtgtt cgacattata gttttggccg gaatggttgt caatattctg cttgctgtgt
     5101 ttctcatcct ctactatttt gattttcttt aacttttttc actttagccc cacacaaaac
     5161 attaacggtt tttggtctta atccgaccgt gcacacctga aaccaatcgt ctcgtctcgg
     5221 aaatcaggaa ccataaaact tctattggtt attctgatat cgactccctg gcttgtatag
     5281 ccactacctc tcatagtctt gtaggtagaa ggagaccgat tactcgtcgg cgtagcagca
     5341 ttttcttgaa ggtagagatc ctcgacatac caattatccg tccattccat aacatttcct
     5401 gcgccgtcat aaactccaaa gacactttta tcaagaggaa aagccccaac cggagccgac
     5461 acaacataac catcggcctc tcctgcaaaa ttggcaggat ggccgctgag accctcaccc
     5521 cacggccagg tgcgaccatc tgtccctcgc atagcctttt cccattcctg ttcggtagga
     5581 agacgcttgc ctctccattg acaatagtca ttcgcatcac tccatgacac ataggtaatc
     5641 ggctgatgag ggcctcgcag caaaccgagc ttttcggcat agcgggaagg cggaccaggc
     5701 tgccgatgtc cggtagcttc aacaaattcc atataccgct ggtaggtaac ctcgtaccga
     5761 tcaatccaat atgcatcgag atgagcgacg tgcgcaggct tttcattgaa tccgccttta
     5821 tcactcccaa gaatgaattc ccctgcagga atttcaacca tctcatcttc atttgcccga
     5881 ccagaaagcg cctcatcctt taatgcctca ggtgatgtct ccgaggctaa agaaggatga
     5941 gattgaagat cgcgatccgg gggagttgga ggagcatcgg ttcccctcaa tattcccaac
     6001 accggcaacc cggtcataaa taacaccgca atcaaaaaaa taagtttaaa tcgaccgtcc
     6061 atgttcccct atttctggtc cgtcaaggtt gcctattccg agaaactatg ggcgattagt
     6121 ctcgccgacg aggagcttga ttattgttta tgaggattga ggattctcat ttggaggatc
     6181 ttttgcgcac cgaaatccaa cactggcatc gttcctccac attttcgcgg caaaacgctt
     6241 ggtcaccctg gcgcctatac gagattcacg ccaagaacct ccacgaatga ctttattctc
     6301 gtcttcgtct tttgggccgg gggggtcccg atacggagct tgctgataat agtctggagc
     6361 ataactatcc atgacccact cagccacatt gccggtgaca tcatagagtc caaatgggct
     6421 tctccctact tcataagatc ccacgggagc taaaaactta aagccatcct cctccccatc
     6481 cacattggca aactgatagt caaacttatc accccaagga taacggcgtt ttccttctcc
     6541 ccttgccgct ttttcccact ccgcttctgt cggcaaccgc ttgccagccc aacgacaata
     6601 cccaaaggcg tcgttccagg tcaccgccac caccggataa tcgggcccga tcaatttgga
     6661 gacatcatct tcaaagaccg gcaccttagg cttgtcccgt ttcgtcatgt tgatatatcg
     6721 ctcataatct gcctgtgtga cttctttaag atcaatgtaa aacgtttgga ggtataccgg
     6781 atggggtggc ccttcatcgg ggtccccatc gccaatgccc atggtaaatg gtccttcagg
     6841 aatttcgacc atttcacgat tatcatcccc cacgagcgtt ttatacatag aaaagtcttg
     6901 tgtgggcatt ggctgcaaca cacggacatt ggccgattga ccagccgaca actcatccaa
     6961 atcttttttc cctttatagg tcataacaat caacatcacg atcatcatga tgaaagagcc
     7021 aaacacaaat agaatggccc ccatgaggac ccgttgattt tccattacta tttttcctct
     7081 cgcttcgacc tactcagcac gatacttcag tttgattagg gttgcatagg accatgatgc
     7141 ttgacgttcg actcaggaag gttctgattt cgttgttgcc aggtctcaat gagcatcgaa
     7201 atccaaattc ccaaaaatcc acccatcgcc gcccctgacg cggcaccaac ggtcacagcc
     7261 tccgttccac tcaatcctaa agccaacagt ccacctaaaa tggtcccgat aaaaatgctc
     7321 aaaaaaaatc cccggccgcc gactaagcct accccggcgc caagaagggc tccggctgtt
     7381 agcgcgacag ggagccatcc ctctcccata aagatcccga tcaacaggcc cactgtccct
     7441 aatcccagga tacccaagag gacgtcaaat attttcgatg gagttacttt cacttttcat
     7501 tcccgcccaa gatcaacgct ggatgattca gattgaaccc gaatagaacc gaaatccacc
     7561 tccactcccg ggccccctaa caaggaaata ccccaggcat gcggggccgg ttcatggtca
     7621 tgaatcttca gaaaatcttc atacacatta acccgctcct caacccactt cccaagggat
     7681 tcagtcccac tgcgaatgac aatttctgtt gagctaaaca tgccaccctc cttgactgac
     7741 cctaccggaa gggtggcact ccacacatat ttcgtgttca cgggaataaa cattaagtcg
     7801 acatccaagg acgggtagat ggcggccagg aaatcggcgt tatcgggaac actctgtaat
     7861 ctccagcgcc aagtgagaat agggtgttgc ttcggatccc acgtcatctg cttggtatac
     7921 acccgttgat tggcgttttt cgcggaaaga aaaaaagccc caccctcttc ttgaatcgta
     7981 taggtttcat gggctgtgac cgtactccgc tgcgcttccc atccttgagg aaatcctttg
     8041 gcatcaggat gctgaaagtc ctccaacacc atcgaactac cggaaggccc tgagctcggg
     8101 acctcttggg cccacgcggc actacttcca tttatcagaa ggcccacgat caaccatgcc
     8161 aaacaagccg cgcttacccg gctccgcttc acaccctcta aattattgtg ttgccaagaa
     8221 cagattctca ttacgcggat tcctcttctg aaatgacctg aatcaccgga gaaggccttg
     8281 caaattcagc cgttcgccgt tctaccactc gctgatccca tcggctaagc caaaacacaa
     8341 aaaagactga cgaccaaaaa acaatcatat tgagggtgac cattttcgcc gcaaatccca
     8401 gggaaggcgt aaatgcccac gaggatcgat cgggcattaa ttcggaaata tgccacccca
     8461 atcggcccac agacctgatg taccccatca accccattac ccaggtaaac gcggcagcga
     8521 ccacaaacag cgccacaatc ccacgcgggg taattcgtcc ccaatgcaca ggcccattga
     8581 tatgggcgcc ccgcaacatt aatcgattca ataatatccc tccaacaacc accgtcgcgg
     8641 tcgtcatccc ttgaggcgat gaaagaccca cccgaacatt tgccggaata tagaacccgt
     8701 aaatggccaa ccacacgata ttggcaattc ccatgagaaa taacatcaat aagaaaatat
     8761 ttccctttga tgcccacttg actgtcatca cacgattagc tcgccgatac agaatgtaac
     8821 tcaacgtggt taaacagatc atgacgttca ccgcaccatt cttcgcggac attacaccga
     8881 attgaccaat gaccgggtgc tgggccgcgc ccatcgcttt catctcactg ccactcatgg
     8941 caatggtatg gggcgtaaac cacacgagga aacacaccat cagcccaaaa aggatcgcct
     9001 taaagtgggg atgaaagcgc tcaccaccct gaaggcgcgc cagggattgc cagatgtaat
     9061 agttaattcc tagaaacaag accccaacca taatcgcttg gaggacaaac agccacgaca
     9121 acatgcctcc catcatcgcc atgcccatcg tttgcctgaa ctcaaatacg gccctcatca
     9181 accagtagcc ggcaaggggc atggggagaa gcgcacacac caccactaca agaaaaacat
     9241 gtccaaccca atcgtaatat gcacggtcct ccgcggtttt ggacatgaaa aaccggtagg
     9301 tcgcataggc gacaaccact gcgccacccg acataatatc cgccaaaaaa cggtgcgtat
     9361 tcagtggatt ccataatgga gagtgcagga gatgccacac attccccaaa aaatgacctt
     9421 gggcatcaac cccggccggg gccatcataa acgaagccca tgaattcgct aacaacaaca
     9481 aaatgactcc ggttgcattt gagagtacac caagagtcat atgcacccac ttcagcgcc
//
LOCUS       DATIAL010000326         3681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1243403, whole genome shotgun sequence.
ACCESSION   DATIAL010000326 DATIAL010000000
VERSION     DATIAL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243403"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VLA60_10160"
     CDS             complement(<1..1371)
                     /locus_tag="VLA60_10160"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HSF09769.1"
                     /translation="MTQSSLPRWWCFATAFSTGAVVMALEILGSRLLAPVFGSSLFVW
                     GALIGVVLAAMSSGYAAGGWLADRYAPQRVLAGLLLLSGAWTLLLAWVGQPVVFGVAQ
                     WIQDPRWGPCFAASVLLAVPAFGLSGVLPALLRLAIGDMGHLGRHTGGMIAVSTIGSL
                     IGTWGTSFYLLTWLGSATLVAILGVVQVCLGLWWTFKVGILQPSLQALLLAGVAGMTW
                     MGFHPVSILPPAIYQEDSPYQQVRVSENDLFRYLILDNTFHAVMWKATPESLALPYSQ
                     VMMGVLPIVENPKRGLILGHGGGSLAKWLGKNWPDLELDVVEMDPSVVRAAERYFGYT
                     PPLNHHVYVEDARTFLRNNPFQYDVVWVDVFARHLIPFHVTTQEFFVELRKHVHPEGA
                     VAVNLASSDSPANVRRAQAVLTTMRLAFPDVVSYGVAGPEWLDTKKGSVNLIFFGGKP
                     VSVMRGS"
     gene            1587..3206
                     /gene="murJ"
                     /locus_tag="VLA60_10165"
     CDS             1587..3206
                     /gene="murJ"
                     /locus_tag="VLA60_10165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HSF09770.1"
                     /translation="MSQSAQPPPAPPSPQGEAHRISRAAGLIGGATFCSRILGFIRDV
                     ILANLFGANASADAFYIAYRIPNLLRELFAEGSMSSAFIPVFTEYHSTRSKQETWELA
                     SAAFTTLLTLVTLVTLMGIVAAPSLVWLLAPGLHGQANQLATTTLLTQIMFPYLLFVS
                     LAALAMGILNSLHSFAVPALAPVFFNLCVIFFAVAISPLFDQAILAVAIGIVVGGLAQ
                     FLMQLPSLHKHGFPLSWNFHPRHPGVKRMGFLLIPSLLGLAVTQVNLTISTILASYFQ
                     GGPTYLFYGMRLIQFPLGIFGVAMATAILPTLSGQAARGAIHELRETVNFGLRMVLFI
                     ILPSMVGLILLRIPIIHVFFEHGAFSAMDTLGTASALLGFSIGLWAFASYRILATAFY
                     SLQDTKTPAIVAVASVGLNIGLSLWLMQWLQYTGLALATACAAMGNTLILLTILGNRL
                     QGLLWKTLGLSLVRTGIALLPLIAVSLWIAGFPLWQTTGQSLEKVAWLTLALVASTIG
                     YFSVHRVFKSEELMHLTLMLRRKIRKPTSTT"
     gene            3278..>3681
                     /gene="dtd"
                     /locus_tag="VLA60_10170"
     CDS             3278..>3681
                     /gene="dtd"
                     /locus_tag="VLA60_10170"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HSF09771.1"
                     /translation="MIAVIQRVRQASVTVESQVIARINRGLVILLGVAQGDTEQDVSF
                     MVQKIPRLRIFPDPQGKMTDALQDINGELLLISQFTLLANTEKGRRPSFESAAPPEEA
                     RLRYQELLEQFRALDLSVQAGMFGKTMVVSLD"
BASE COUNT          927 a         1057 c          839 g          858 t
ORIGIN      
        1 cgaaccacgc ataacagaga caggttttcc accaaaaaaa atcaggttca cggatccttt
       61 ttttgtatct aaccattcag ggcccgcaac accgtaggac accacatcag gaaacgccag
      121 gcgcatcgtg gtcaacacgg cttgtgcacg gcgaacattc gcgggcgaat cggaagacgc
      181 cagattaacg gcaaccgcgc cttcagggtg cacatgcttc cgtaattcca caaaaaattc
      241 ctgagtcgtg acatggaacg gaataagatg cctggcaaac acatcgaccc acaccacatc
      301 atactggaaa ggattgtttc gtaaaaacgt gcgggcgtct tccacataaa catgatggtt
      361 gagcggtggc gtataaccaa aataccgttc agcagcccgg accacagacg gatccatttc
      421 gaccacatct aattccaaat ccggccagtt cttccctaac cactttgcca atgatcctcc
      481 gccatggcct aaaatcaatc ctcgcttagg attttcgaca attggcaaca cacccatcat
      541 gacttggcta tagggcaagg caagagattc cggcgtcgct ttccacatca ccgcatggaa
      601 agtattgtcc aaaataagat accggaataa atcattttca ctcactctaa cctgttggta
      661 cgggctatcc tcctgataaa tggccggcgg caaaatgctg accggatgaa aacccatcca
      721 agtcattccg gctactcccg caagaagcag ggcctgtaga ctcggctgga gtattccaac
      781 tttgaaggtc caccatagac caagacatac ctggaccacg ccaaggatag cgactaaggt
      841 ggcactaccc agccaggtta aaagataaaa ggacgtcccc caggttccga tgaggcttcc
      901 gatggttgaa accgcaatca tgccaccggt atgacgtcca agatgcccca tatccccgat
      961 agccaaacgc aagagggccg ggagaacgcc gctcaatcca aatgccggaa ccgccaatag
     1021 cacactggct gcaaaacatg gcccccagcg aggatcctga atccattggg ccactccaaa
     1081 aacgacgggt tgccctaccc atgccaataa caaggtccaa gcgccggaca gcaacaacaa
     1141 cccggccaac acccgctgag gagcatatcg atcagccagc cagcctccag cggcatatcc
     1201 actgctcatg gcagccagga ccaccccgat cagcgcgccc cagacaaata gcgaactgcc
     1261 aaaaaccggc gcgagaagtc ggcttcctaa aatttccaac gccatcacca ccgcaccggt
     1321 cgagaacgcg gtcgcaaagc accaccatcg tggcagactg gattgagtca acgaggatct
     1381 tgtcataaga tggagaattg gatggacaaa agggaattga tgctaaagca tgatttagag
     1441 ggtgtcaatt cagaaacagg cgaaaaaatc cgtatccagg ctctgtgtgc ggacccgggc
     1501 ctttaataat acttgagcaa gacaatcgcc ctctgacaac tgacaggtga aacaactcaa
     1561 ccctttccgg taacattccg attcggatgt cccaatcagc tcaacctcca ccagccccgc
     1621 catctccaca aggagaggcc catcgtatta gccgtgcggc cggactgatc ggcggagcca
     1681 ctttctgcag tcggattctt ggatttattc gagatgtcat tctcgccaat ttatttggcg
     1741 ccaacgcttc agcagacgcc ttttacatcg cctatcgaat tcctaacctg ttgcgagaac
     1801 tttttgcgga aggatccatg tcttccgcct ttatcccggt gttcaccgaa tatcattcga
     1861 cccgctccaa gcaggaaacc tgggaattag ccagcgcagc attcaccacg cttctgacgc
     1921 tcgtgaccct tgtcacctta atgggcatcg ttgccgctcc aagtctcgtc tggcttcttg
     1981 cgccaggcct ccatggtcaa gcgaatcaac tcgccacgac cacgctcctc acgcagatca
     2041 tgtttcccta cttactcttc gtgagccttg cggccctggc catgggaata ctcaattccc
     2101 ttcattcatt cgctgttcct gccttggctc ctgtcttttt caatctgtgt gtcattttct
     2161 ttgcagtggc catttcgccc ttatttgatc aggcgattct cgctgtggcc attggaattg
     2221 tggtgggagg gctcgctcaa ttcctcatgc aattacccag tttacacaag catggcttcc
     2281 cattgtcctg gaattttcat ccccgccatc ccggagtgaa acgaatgggt tttctgctca
     2341 ttccttcctt gttagggtta gctgtgaccc aagtcaatct caccatcagc acgattttag
     2401 cttcgtattt tcaagggggc cccacgtatc tgttttatgg gatgcgcctc attcaattcc
     2461 cgttagggat atttggtgtg gcaatggcca cagctatttt gccaacctta tccggccaag
     2521 cagcccgtgg agccattcat gaactacggg aaacggtaaa tttcggatta cgcatggtgc
     2581 tatttatcat ccttccatca atggttgggc tgatcctatt acgcataccc attattcacg
     2641 tgtttttcga gcatggcgcc ttttcggcca tggacacgct tggaaccgct tcggcccttc
     2701 tcggattttc aatcggattg tgggcattcg ccagttatcg aatactggca accgcgtttt
     2761 attcccttca agatacaaaa acacccgcca tcgtggctgt agcttcggtt ggcctcaaca
     2821 ttgggctgtc attatggctt atgcaatggc ttcaatacac cgggctagcc ctggcgaccg
     2881 catgtgccgc aatggggaac acccttatct tactgaccat tttggggaac cggcttcagg
     2941 ggttactgtg gaagacactc ggcctatccc tggtccgcac aggaatagcc cttctccctc
     3001 tcatcgccgt ttctctctgg attgcaggat ttccgctatg gcaaacaaca ggacagagcc
     3061 tcgaaaaagt tgcctggttg actctggcgc ttgttgcaag cacgattgga tatttttccg
     3121 ttcaccgtgt attcaagtca gaggagctca tgcacctgac gctcatgttg cgacgaaaga
     3181 tacgcaaacc aacttctaca acgtaagcat acggactgac aggcataccg tcatcatgcc
     3241 cggctgtcct taaataagcg atatcacgaa agggctcatg attgccgtta ttcaacgcgt
     3301 caggcaggct tcagtcacag tggaaagcca agtcatcgcc cgcatcaacc gggggctcgt
     3361 cattctgtta ggtgtggccc aaggcgatac cgaacaagac gtgtccttca tggtccagaa
     3421 aattcctcgc ctacgaattt tcccggaccc acaagggaaa atgactgatg cccttcaaga
     3481 catcaatggc gaactcctcc tgatctctca atttacctta ctggctaata ccgaaaaagg
     3541 acgacgccca agctttgaat cggcagcgcc tccggaagaa gcccggttgc gatatcagga
     3601 attactggaa caattccgag ccctcgatct gtctgtacaa gcaggaatgt ttgggaagac
     3661 aatggtcgtg tcattggaca a
//
LOCUS       DATIAL010000327         4857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_384607, whole genome shotgun sequence.
ACCESSION   DATIAL010000327 DATIAL010000000
VERSION     DATIAL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384607"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VLA60_10175"
     CDS             <1..542
                     /locus_tag="VLA60_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09772.1"
                     /translation="TTHLIDAVAAHHGLTVHETPVGFKYIGELLAKGEVVFGGEESAG
                     MSIKGHVPETDGILAGALVAEMVAFTGQTIQELLKDLFDRVGAAFTARQDLPMSEHLK
                     TQVKQVLEHPPSEFAGAEVIHVNKLDGCKLILPDDSWYLIRLAGTESLVRCYGEAKTP
                     ERLEEIMQAGRELLHTPHN"
     gene            complement(731..1057)
                     /locus_tag="VLA60_10180"
     CDS             complement(731..1057)
                     /locus_tag="VLA60_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSF09773.1"
                     /translation="MRSCINQGSLVACEVVWAEVSAVFPSESDARDVLQTLGVQFSPL
                     RIDASLFAGRLWKDYRQKGGRRDRMVADFLIGAHALYHADALLTRDRGFYRSGMPKLK
                     IIDPSP"
     gene            complement(1134..1364)
                     /locus_tag="VLA60_10185"
     CDS             complement(1134..1364)
                     /locus_tag="VLA60_10185"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HSF09774.1"
                     /translation="MKTTISEKGQVTIPKPLRDRLGLLPGQVLDFREEKERLVATKAH
                     TSDPVGALFGVLKTKQRTDQTMTALRGKPGPK"
     gene            complement(1682..2146)
                     /locus_tag="VLA60_10190"
     CDS             complement(1682..2146)
                     /locus_tag="VLA60_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSF09775.1"
                     /translation="MTQDFVIRKTGRKVLDVSQIERTAKEGVSMDEQSLDVKIESRNV
                     GMTPRWKAEIERRTEALQTDTIRIIHARVTLTKNAHHKKGADNAEALVVVTLPRRRTV
                     TARKESKTFEEAIRSAFQAIEHELEKVEEKRLARNAKATAKRIEKAMALTAV"
     gene            2352..2837
                     /gene="msrB"
                     /locus_tag="VLA60_10195"
     CDS             2352..2837
                     /gene="msrB"
                     /locus_tag="VLA60_10195"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HSF09776.1"
                     /translation="MKIGMALGFGVLAKTLGVALAAPTTPTGGSDITKITKTDEEWKA
                     LLTPMQYRVLRHEDTEPPFTNEYHQSKAKGIYQCAGCALPLFSSDTKFDSGTGWPSFW
                     QPISEKVVETRTDWKLIYPRTEVHCARCGGHQGHIFDDGPPPTGLRYCINSAALKFVP
                     A"
     gene            complement(2803..>4857)
                     /locus_tag="VLA60_10200"
     CDS             complement(2803..>4857)
                     /locus_tag="VLA60_10200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF09777.1"
                     /translation="TAMKPKTNDSQFRFVGHWATSSFPRHVFADVWNIIQSKDRDQLS
                     KLFRGKVVILFSPVSKKPTLSTPWESVVPVEFVHALLANAVLTNGWISRTPLWVAFPA
                     TASAGLFFVCFLLWETPPTRLGMMGLAGTLLTGLALLWGLHSGWVWPILSTGLALGMT
                     VGGSLVWRLSRSRSIIQRRIAQGRQQLQQLETELAKRQQHVRELETRLHVAQDHAHRS
                     ATVIEGLETQQGGVSRQLECSQAEVEETRRQIDRLQTELEDLRRQVPAMQHAEHGHPE
                     SCENQVLLQECESLHILTRAPSVLRVFQDLKKASGTQSPILLLGETGTGKEVFARAAH
                     ALSPRHRGPFVSVNMAAIRPELFEGELFGHVKGAFTGAVGREGYIEAANGGTLFLDEV
                     GELPSDLQAKLLRFLEDGSFHRIGESRLTQVDARIIAATNCNLQQDVEAGRYREDLYY
                     RLRSIVLTLPPLRERGEEDCLLLAQSFLQYFSRQQNRMDLRFTQGALDVIMAYRWPGN
                     IRELRQTVAQAVALANGSLVTEADLHLSSPVMPSLKGEGHGRVELERLEDDMVLECLR
                     RHGFDMQVTAKALGWDRSTVTQRLKGLGFQALVDYHGNIEAAAQMLAGDKTLSPLVAR
                     RLREYSKNLLPSSRHYTSAEEAVADCRKRFRNLSERHFPAVEQLVHQRFATPEQISTP
                     RN"
BASE COUNT         1226 a         1312 c         1148 g         1171 t
ORIGIN      
        1 ccaccaccca tctcattgat gccgtggcgg cacaccatgg actcaccgtc cacgaaaccc
       61 ccgtcggatt caaatacatc ggcgaattac ttgcgaaagg ggaagtggtc tttggcgggg
      121 aggaaagcgc cggaatgtcc atcaagggcc acgtaccgga aacagatggg atattggccg
      181 gggcgttggt cgcagagatg gtggccttta cgggacagac cattcaggag ttactgaaag
      241 acctctttga ccgagtggga gctgcgttca ccgcccgaca agatttgccg atgagcgaac
      301 acctcaagac acaggtcaag caggtccttg aacatccacc atcggagttt gcgggagcag
      361 aggtcatcca tgtcaataaa ctggatggct gcaaactcat cctgccggat gactcctggt
      421 atctaattcg attggccgga accgaatccc tcgtccgatg ctatggcgaa gccaaaaccc
      481 ctgagcgcct ggaggaaatc atgcaagcag gacgggaact actgcatact cctcacaatt
      541 gaatgttcac aggcttacaa cccatggacc tctccctctc ctcctcgata ccctggatcc
      601 tgtttagtaa accaacttta tggataggtt agggccttta gcgtgaagta gatttgacaa
      661 agggaaaatg aggtgggtca ggccattgac cggagaaatc cagacacagt agaaaaaatt
      721 tttcaactct ctatggagag ggatcaataa tcttcagctt gggcattccg gatcgataaa
      781 accctcggtc tcgagtcaac aacgcatccg catggtaaag ggcgtgagcc ccaatgagaa
      841 agtcagccac catacggtct cgccgtccgc ccttttgccg ataatccttc cacaatcgcc
      901 cggcaaacaa tgaagcatca atccttaagg gagaaaactg cactcccaaa gtttgcaaaa
      961 catcccgggc atcgctctca gaaggaaaaa cagccgaaac ctcggcccat acgacctcgc
     1021 aagctactaa agatccttga ttaatacacg aacgaatagc ctcttttgag tgaacccata
     1081 catggggtct gccccaaaaa gaacaataag gatattcgta tcaacggccg taatcacttg
     1141 ggaccaggtt tccctcgtaa tgccgtcatg gtttgatcag tgcgttgttt ggtttttaat
     1201 acgccaaaca atgctcctac cggatcagac gtatgagctt ttgtggcaac cagcctctct
     1261 ttctcttctc gaaaatccaa tacttgtccg gggagaagac ctaaacggtc tcgaagaggt
     1321 ttggggatcg tcacttgtcc tttttcagag atggttgttt tcatactttt actgtaagaa
     1381 ttcctactca tttggtcaaa ttacatacaa ttatccgtag caaatccaga agaatcactc
     1441 tcatgttctt cttcgttcct ccaaaatggt ggggccgcaa ctcacaaatc taatcaggtt
     1501 ttattcaata taagatagcg aaggaacccc ccttcgcttg atgagggggg tcacttcctt
     1561 cagctattag aaagttattg gttcgagtgg taaggaataa aaaagcgggt ggacggacgg
     1621 cccacccgct acgactcgtt cggatgaaga gaaaaagaat agagatgaag gttttacccc
     1681 atcagacggc ggttagcgcc atagcttttt cgattctttt ggctgtggcc ttggcattgc
     1741 gggccaaccg tttttcttca actttttcga gttcatgctc tatggcctga aaggctgagc
     1801 gaatggcttc ttcaaaggtt ttcgattctt ttctggcggt caccgttcga cgccggggaa
     1861 gcgtcacgac aaccagcgcc tccgcgttat cagccccttt tttatgatgg gcatttttcg
     1921 taagagtgac acgggcatga atgatgcgaa tggtatccgt ttgcaaggcc tcggtccgtc
     1981 tctcaatttc ggctttccac cgaggtgtca tgccaacgtt ccggctctca atttttacat
     2041 ccagcgattg ttcatccata gacactcctt ctttggctgt tcgttcaatc tgtgaaacat
     2101 ccaacacctt ccggcctgtt tttcggatga cgaaatcctg agtcaatcct ccttttcttt
     2161 tggttttcta cagtgtaaaa atttctcttt cttcagtcaa aggtctcctc accaaatctg
     2221 taattttttt tctcattgca agactgatac agtatatcag ggtgggtgga atacggaggc
     2281 gtttccaccc tctcagctca gaggagggct atcagatgtc tataaatgta aacgtcaaca
     2341 ggagaaatct tatgaaaatt ggcatggcat tagggttcgg agttctcgcc aaaactctcg
     2401 gcgtggcatt ggccgctccc acgaccccaa ccgggggttc ggatatcacc aaaatcacga
     2461 aaacggacga agagtggaag gcgctgttaa cccccatgca ataccgtgtc ttgcggcatg
     2521 aagacaccga gccgcccttt accaatgaat atcatcaaag taaagccaaa ggtatttacc
     2581 aatgcgcagg atgtgcgttg ccactctttt cttccgacac gaaattcgac agcgggaccg
     2641 gatggcccag cttttggcaa ccgatttcgg aaaaagtagt cgaaacccgc accgattgga
     2701 aattgattta tccacgaacc gaagtccatt gcgcccgatg cggtggccac cagggacaca
     2761 tatttgacga cggcccaccg ccgacggggt tacgctattg cattaattcc gcggcgttga
     2821 aatttgttcc ggcgtagcaa agcgctggtg caccaactgc tcgactgccg gaaaatgtcg
     2881 ctctgaaaga ttgcgaaacc gttttcggca gtcagcgacc gcttcttcag cagacgtgta
     2941 gtgtctggaa gaaggaagca ggttttttga atactcgcgc agccgtctcg cgaccaaggg
     3001 ggacaaggtt ttatccccgg ccagcatctg cgccgccgct tcaatatttc catgatagtc
     3061 gactaacgcc tgaaatccga gacctttcaa tcgttgagtg acggtgctgc gatcccaccc
     3121 taaagcttta gctgtcacct gcatatcgaa tccatgacgg cgcaggcatt ccaacaccat
     3181 gtcatcctcc aatcgttcca gttccacccg accatgccct tcacccttca aggacggcat
     3241 gacggggcta ctgagatgca aatctgcttc ggttacgaga gacccgttag ccaacgccac
     3301 tgcctgggcc actgtttgtc gaagttctcg aatattgccc ggccatcgat aggccatgat
     3361 cacatccaat gccccttgcg tgaaccttaa atccattcgg ttctgttgcc gggaaaaata
     3421 ctgcaggaag gattgagcca atagcaggca gtcttcctcc cctcgttccc gcagaggagg
     3481 cagagttaac acaatactcc gtaatcgata atagagatct tcccggtatc gccctgcctc
     3541 cacatcttgt tgaagattgc aatttgtcgc cgcgataatt cgtgcatcca cctgagtgag
     3601 ccggctttct ccaatccggt gaaatgaccc gtcctccaga aaccgcagta attttgcttg
     3661 caaatcggat gggagttcgc cgacttcatc caaaaatagc gttccaccat tcgccgcttc
     3721 aatatagccc tcacgaccaa ccgcgccggt aaaggcccct ttcacatggc cgaacaattc
     3781 tccttcaaat aactccggcc ggatcgcggc catattcacc gacacaaatg ggccacgatg
     3841 ccgaggactc aatgcatggg cagctcgagc aaacacctct ttgcccgtgc ccgtttctcc
     3901 caatagaaga atggggctct gggtcccgga ggcttttttt aaatcctgaa aaacgcggag
     3961 gaccgaaggg gcacgggtca gaatatgaag cgactcacat tcttgaagta atacctgatt
     4021 ctcacaggat tcggggtgcc catgctcagc atgctgcatg gccggcacct gccgacggag
     4081 atcctccaat tcagtttgca accggtcaat ctgtcgtcgg gtttcttcta cctcggcttg
     4141 agaacactct aattggcgag acaccccccc ttgctgagtt tctaaacctt caataaccgt
     4201 cgccgaccgg tgtgcatgat cttgtgccac atgcaagcga gtctcgagtt cccggacatg
     4261 ctgctgtctc tttgccagct ctgtttccaa ttgttgtagc tgctgtcgcc cttgagcgat
     4321 ccgccgttgg ataatcgacc gggaccggga caaccgccaa accagcgatc cccctacggt
     4381 cattcctaaa gccaatcctg tactcaagat cggccacacc caaccactat gaagtcccca
     4441 tagcagagca agccccgtaa gaagagtacc ggctagaccc atcatcccca ggcgggttgg
     4501 cggagtttcc caaagaagga aacagacaaa gaacaggcct gcactcgctg ttgcaggaaa
     4561 agcgacccac agaggagtcc tggaaatcca tccgtttgtc agtaccgcgt tggccaataa
     4621 ggcatgaaca aattctaccg gtaccaccga ttcccacggg gttgaaaggg ttggtttctt
     4681 cgacacagga gaaaaaagga tcactacttt tccgcgaaac aatttcgata attgatcacg
     4741 atctttgctc tggatgatgt tccacacgtc cgcaaagaca tgcctgggaa aggatgaagt
     4801 tgcccaatgt ccaacaaacc ggaattgaga gtcgttggtc ttcggtttca tagctgt
//
LOCUS       DATIAL010000328         5664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_598857, whole genome shotgun sequence.
ACCESSION   DATIAL010000328 DATIAL010000000
VERSION     DATIAL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598857"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VLA60_10205"
     CDS             complement(<1..503)
                     /locus_tag="VLA60_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09778.1"
                     /translation="MKDKTWKPKRVGDFPITPSGIPVIELSIRSAECKATLESLIRET
                     DGVRLKDNQDSAVPALMIMELEENPEDTFSLIREMMKLNKAMEIFLTASRLESNILLE
                     AMRAGAKEFLAQPLQKQDVSDAIMRFVERSTAPVRQGDGQRAPGKVLAFFGGKGGVGT
                     TSIAVNLA"
     gene            complement(500..937)
                     /locus_tag="VLA60_10210"
     CDS             complement(500..937)
                     /locus_tag="VLA60_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09779.1"
                     /translation="MKKEQSMQRQSIVTMVGLIGLVLVGMGGCGVWPVMTEENGRRPQ
                     AMGKSDQVLSPWAPQPVHLNEEYGLAYRQALENQIVNPAASNKIDPIPGAADSRALQN
                     SMTRYQLMFQSPPFSEFKLQGGSGSSASTGYSSTGMGAGGTTK"
     gene            complement(946..2445)
                     /locus_tag="VLA60_10215"
     CDS             complement(946..2445)
                     /locus_tag="VLA60_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HSF09780.1"
                     /translation="MNQTHGFPFRSMLMALGLVLVGYTGALGEGLTTQAVHVNEPQNL
                     RLTVGESKIVERETAFKRASVANPKVADQIVLSTKQIYLTGLEVGTTTLTLWGQDGQV
                     SNVFQVRVSPDVTRLKEQLHTILPNEPGLQVMSSHEHISLTGNVSNMESLTKALTLAE
                     PYAPNKIINLVQVGGVQQVMMEVKVAEMQRGLMRRLGVNFQRQQKNHRDFSIGLINKL
                     STVDAFDPLPHVQSSQTTTLEFPSVRTLASNLILGFGIGDDLWTLFLDMLKEHSLSKT
                     LAEPTLIAESGQAAEFLVGGEYPVPIPQQLGQVTIKYKEFGVGLKFTPTVLSEGRISV
                     IVNPEVSELDFANGVQLNGFFVPALSTRRVKTVVELGDGQSFAIAGLLQDNIKETVSK
                     YPFLGDIPILGALFRSTSFQKNETELIVIVTPHLVKPLDMVKQTLPTDHYLEPNDFEL
                     MLMGYVEGVFPEPNVARPGEGYTLGSSSVAGRMPYRKGGMEGMFGHLAP"
     gene            complement(2492..3337)
                     /gene="cpaB"
                     /locus_tag="VLA60_10220"
     CDS             complement(2492..3337)
                     /gene="cpaB"
                     /locus_tag="VLA60_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HSF09781.1"
                     /translation="MGQKRPLVFLGIAMIIALVTTVMVYRWLQGQRMIEVDVPEVVME
                     GVSVAVASVDIPWGTPLGEKAVRVMTYPEEGVPVGHFKDTASLKGRVVLMNLKKNEPI
                     LESKLAPIDLKTGGVSAVMDPNKRAMAVKVNEIVGLPGFVQPGDRVDVMATFDDPRGK
                     KKEGGGNREEVTKVVLENTLVLAIDTQMERSAGEEKPTPVKVITLEVSLEEAEKLAMA
                     ENGGKLRLALRSPLNPEVKKTKGADFKDLMSSHQLIPPRPKAAHKPENQVEVIKGTDR
                     KTMKF"
     gene            complement(3519..4046)
                     /locus_tag="VLA60_10225"
     CDS             complement(3519..4046)
                     /locus_tag="VLA60_10225"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HSF09782.1"
                     /translation="MSSFLLLSGLALGLVVAAVTDVREGKIPNWLTFSLAGFGMGVLS
                     WEHGWDGFLFSLGGLGMGLVCLMFFYIKGGMGAGDVKLLGAIGAILGPGQVVFAFAFA
                     AMLGGLYSLALLSNQGGMRHAWDRMFFLLSTLKVTRTIPVSAAPISTEPKLRYALVLG
                     LGTVIAKTLFYYGVL"
     gene            complement(4556..>5664)
                     /locus_tag="VLA60_10230"
     CDS             complement(4556..>5664)
                     /locus_tag="VLA60_10230"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TadG family pilus assembly protein"
                     /protein_id="HSF09783.1"
                     /translation="KQRSFGVCALLNQRGIAAINGVFMGLTMAIMAGVAIDVGHALLT
                     QNELHNASDAAALAAARQLGVTYLAMPIDQQQDLNRNLTSNEQSQINAQATAAAFANS
                     ASNISNLSLSQGDIEYGTWDFNAKTFTPTVTRPNAIRATTRRDGAANGPITTFFGGIL
                     GVNTMSLATTSIAALGTSGGPTPPGVGDVPFAISSNWFNGVASCNSGIQFSPTGVNGC
                     AGWHVFDQHPANASKLRATVDGLEDGSYESPGMTPGQTQFTFTGGEVSSAFPNLINLW
                     DTKKQWNNDTGRDEWAINLPVYGASSSSSCNNPSGDITIVGYAKAVITTVKKNDIQAE
                     VKCDTFFDGAPNPNQTGGGLGPLQPISVYPRLVS"
BASE COUNT         1428 a         1595 c         1266 g         1375 t
ORIGIN      
        1 gccaaattga ccgcgatact ggtcgttccc actccacctt ttcccccaaa aaatgcgaga
       61 acctttcccg gcgctcgttg tccgtcaccc tgacgaactg gagccgtgct tcgctccaca
      121 aatcgcataa tcgcatcgct cacatcttgc ttttggagcg gttgagccaa aaactctttg
      181 gctccggccc gcatggcttc caataaaata tttgactcca gccgggaagc ggtcagaaat
      241 atctccatcg ctttattcaa tttcatcatc tctcgaataa gagagaacgt gtcctccgga
      301 ttctcttcaa gttccataat catgagtgcg ggaacagcgg agtcctgatt gtccttgagt
      361 cgcacaccat ccgtctcccg aataaggctt tcaagagttg ccttgcattc cgcagaacga
      421 atagaaagct caatgaccgg tataccggat ggggtaattg ggaaatcccc tacacgcttt
      481 ggcttccaag ttttatcctt cattttgtcg ttcctcctgc ccccatacct gtcgatgagt
      541 acccggttga cgcactacta ccagacccac cctgcagttt aaactctgaa aatggcggac
      601 tttgaaacat taactgatag cgagtcatac tattttgaag ggcccgtgaa tcggccgctc
      661 ccggtatggg atcaattttg tttgatgcag cgggattcac gatctgattc tcaagagcct
      721 gacggtaggc cagtccgtat tcctcattca aatgaacggg ctgaggcgcc cacggcgaga
      781 gaacctgatc gcttttcccc atcgcttggg gacgccgacc attctcctcc gtcatgactg
      841 gccacactcc acagccaccc atccccacca gaaccaggcc gatcagaccg accattgtta
      901 caattgattg gcgttgcata ctctgctcct ttttcatcaa atttcctatg gggccagatg
      961 accaaacatg ccttccatcc cacctttgcg atacggcatt ctcccggcca cactcgatga
     1021 tccaagggtg tagccctcgc cgggcctggc gacatttggc tccggaaaca ccccttccac
     1081 atatcccatc agcatcaact caaaatcgtt aggttctaaa tagtgatcag tgggtaaggt
     1141 ctgcttcacc atgtccagag gtttcaccaa atgaggggtc acaatcacaa tcagttcggt
     1201 ctcatttttt tggaacgagg tgctccggaa taaggcaccc aagatcggaa tatcacctaa
     1261 gaaaggatac ttcgagaccg tttctttaat attatcttgg agaagtcccg caatggcgaa
     1321 actttggcca tcgcctaatt ccacgaccgt cttgacccgt cgggtcgaaa gggccggcac
     1381 aaagaacccg tttaactgaa ccccattcgc aaaatctaat tctgacactt ccggattcac
     1441 aatgacggag atgcgcccct ccgaaagaac agtgggcgta aatttcaatc ccactccaaa
     1501 ttccttatat ttaatcgtca cctggccgag ttgttgcgga ataggaaccg gatattctcc
     1561 cccaaccagg aattccgctg cttgcccgct ctcggcgatc aaggtcggtt cggctaatgt
     1621 tttagacagg ctatgctcct tcagcatatc taaaaagagg gtccataaat catcaccaat
     1681 cccgaatcct aaaatcaggt tcgaggccag cgttcggaca ctcggaaact ccagggtcgt
     1741 agtttgtgag gattggacgt gtggtaatgg atcgaaggca tcaaccgtgg ataatttatt
     1801 aatgagtccg atagaaaaat cacgatgatt tttctgttgc cgttgaaaat tgacccctag
     1861 gcgcctcatc aacccgcgct gcatttccgc cactttcact tccatcatca cctgttgcac
     1921 gccacctacc tgcaccagat taatgatttt attgggggca tagggctcag ccagagtgag
     1981 ggctttcgtc aaagactcca tattggagac attgcccgtg aggctaatat gctcgtggct
     2041 gctcatcact tgaaggccgg gttcattcgg caatatggta tgaagctgct ccttcagacg
     2101 agtcacatcg ggagagaccc ggacctgaaa aacattggac acctgcccat cttgccccca
     2161 tagggttaag gttgtcgttc caacctcaag cccggtcaaa taaatttgct tggtggagag
     2221 cacaatttga tccgctactt taggattggc tactgaggca cgcttaaacg ccgtctcccg
     2281 ctccacaatt tttgattctc cgaccgtgag ccgtaggttt tgaggctcat taacgtggac
     2341 cgcctgagtg gtgagaccct cccccaaggc tcccgtgtag cccacaagga ccaggcccag
     2401 agccatcaac atgctcctga atggaaaacc gtgagtctga ttcatatccc tgcctttcct
     2461 aacgatcatc gcgtcaccct cgacgaccct cttaaaattt catcgttttt cgatccgtcc
     2521 ctttgatcac ctcgacctgg ttttcaggct tgtgagccgc tttcggccgc ggaggtatga
     2581 gttggtggga actcatcaaa tccttaaagt ccgccccctt cgttttcttc acctcgggat
     2641 tgagcggact ccgcaaggcc aaacgtaatt ttccaccatt ttcagccatg gccaacttct
     2701 cagcttcttc caatgacact tccaacgtta tgactttcac gggagtgggc ttttcctctc
     2761 cagctgatcg ttccatttgc gtatcgatcg ccaacaccag ggtattctca agaaccactt
     2821 tcgtgacttc ctctcgatta ccaccaccct ctttcttctt tcccctcgga tcatcaaacg
     2881 tcgccatgac atctacccga tcgcctggct gtacaaaacc cggaagcccg acgatttcgt
     2941 tgactttcac ggccatcgcc cgtttgttgg gatccatcac ggccgacacg ccgcccgttt
     3001 taagatcgat gggagctaac ttggattcta aaatcggttc attcttcttc agattcatta
     3061 acaccacccg gcccttcaag gaagccgtat ccttaaaatg gcccacgggg accccttctt
     3121 cagggtacgt catcactcga accgcttttt caccgagtgg cgtcccccag gggatatcga
     3181 cactggccac cgcaacactc acaccctcca tcaccacttc agggacgtcg acttcgatca
     3241 ttcgttgccc ctgtagccac cgatacacca tgaccgtcgt gaccagggca atgatcattg
     3301 caattcctaa aaatacgagt ggacgttttt gtcccatgag acttcctccc ccttcttcac
     3361 caaatctaga ccgaattttt tattcatgcc tatcaatcct gaggtcccga atcctctacc
     3421 tctgcatatc ggatggcaac tgacattctt aatagacaac gaaaagaatc ttctccgatt
     3481 gctcattttc aactcgcaga cctaagcgaa tatgcgagtt acaagacacc ataataaaac
     3541 aacgttttgg cgatcacggt tcctagtcct aaaaccaggg cataccgtaa cttaggctcg
     3601 gttgaaattg gcgcagcaga aaccggaata gtcctggtca ccttcagggt tgaaagaaga
     3661 aaaaacatcc gatcccaggc atggcgcatg ccaccctgat tggacaacag cgcgaggcta
     3721 tagagtcctc ccagcatggc ggcgaaggca aaagcaaaca ccacctggcc cgggcccaag
     3781 atcgcaccaa tggctccgag caatttcacg tcccctgcgc ccatcccccc tttaatataa
     3841 aaaaacatca agcagaccaa ccccatgccc aaacctccga gactgaacag gaaaccgtcc
     3901 cagccatgct cccaactcag tacacccatt ccaaagcctg cgagagaaaa agtcagccaa
     3961 ttgggaatct tgccctcccg aacatctgtg acggcagcga ccactaatcc caaggctagt
     4021 ccggataaca agagaaaaga actcatggtt tcagaatgtc ccgtcaaagg ctttttacga
     4081 atttactgtt gaaatctaat agaagctcat gtggtaacga ccgacccaga aagaacggaa
     4141 gccaccacca gtcctaaagc aaagtcactg tctacaagtt cctgtttcgg tgaattacgg
     4201 aaccttctta aggctgtcac caaaaaagtt aggaacgagg atcagagaga cgacgtggtc
     4261 gatccagaaa aagggcgagg agatgttctg cggaaaattt tgggaaaatg ccaaaagggt
     4321 taaaacaaga agtggcttgt cactatctcg gaggatcggg gagtttttga tgagcaggtt
     4381 tcttcggaca attgcctgcc gtaaatgagg acaactatgg tgagttgagc ctggaaagcc
     4441 aatatacgaa aaattttagc aaaaaaggga aatagataaa ttcctttgag ccagtcttgg
     4501 ttcagacggg cctgaaggaa aagaaatacc caaactctcg cctgactcct ccccattacg
     4561 acaccagtcg tgggtatacc gaaatgggct gaaggggtcc aaggccccca ccggtctggt
     4621 tgggatttgg cgctccatca aaaaaggtat cgcatttcac ttccgcttga atatcatttt
     4681 tcttaactgt tgtaatcacc gctttcgcat atcccacgat ggtgatatcc ccgctgggat
     4741 tattgcaaga ggatgaggaa ctggctccat agacaggcag gttgatggcc cattcatcac
     4801 gtccggtgtc gttattccat tgtttcttcg tatcccacag attaatcaga ttgggaaatg
     4861 cggaagagac ttctccaccc gtaaaggtga actgagtctg ccctggagtc atcccaggac
     4921 tttcatagct tccgtcttct aacccatcga cagtggcacg gagcttactc gcattggccg
     4981 gatgctgatc aaacacatgc catcccgcac agccattcac gccggtgggt gaaaactgga
     5041 tgccggaatt gcaactggcc acgccattaa accagttact ggaaatcgca aacggtacat
     5101 cgcccactcc cgggggggtt ggaccacccg aggtccccaa tgcggcaatt gaggtggtgg
     5161 ccagactcat ggtattgacc cccaatatac ccccaaaaaa agtcgtaatc ggtccattcg
     5221 ccgcaccatc ccgccgtgtg gtcgcgcgaa tggcattggg gcgtgtcacg gtgggggtga
     5281 acgtcttggc attgaaatcc catgtgccat attcgatatc tccctgggaa agagataaat
     5341 tactgatatt cgacgcacta ttggcaaagg ctgcggccgt cgcctgcgcg tttatttgtg
     5401 attgttcatt gctcgttaga ttccgattca aatcctgctg ctgatcaatg ggcatagcca
     5461 gataggtcac ccccaattgc ctggcagcgg ccagggcagc ggcatccgag gcattgtgca
     5521 attcattttg ggtgagcaaa gcatgtccga catcaatggc cacacccgcc atgatggcca
     5581 tggtcaatcc cataaacacg ccgttaatgg ccgcgatacc acgctgattc aggagtgcac
     5641 agacaccgaa tgatcgttgc ttgt
//
LOCUS       DATIAL010000329         9351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_385295, whole genome shotgun sequence.
ACCESSION   DATIAL010000329 DATIAL010000000
VERSION     DATIAL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385295"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(408..1463)
                     /locus_tag="VLA60_10235"
     CDS             complement(408..1463)
                     /locus_tag="VLA60_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HSF09784.1"
                     /translation="MRVQHMPTTIRPVFFLCALILCVLPGLASANTEELAPSEAVVSS
                     VWQVALDGSGHFTSIQEAIDQAASGDTILIKRGAYAEDVTVHSKEGLSIIGEGMDLVV
                     LTGKKRVGTLHIGKWPYGATNVTIQDLSVIQHGGLGVGIFNGSGVRLIRIRVNGMVFS
                     QQVQDVLLEDCVIGDSETTGVAFADSTGTLSGNLIYHNDHGVAIGGNSDVTLRQNVIT
                     RSLYEAVLMTDQSKARLIQNTLAQNGGGAAFHDDAQADVQGNIISQSAIGFLFFPGSH
                     TTLAFNALSGNQGDYVMTGTPPTPAPERAGKTDVQFAPGFVSPENGDFRLQSDSSMIQ
                     VGAFPYLGALPPVSSNP"
     gene            1568..2263
                     /gene="coaE"
                     /locus_tag="VLA60_10240"
     CDS             1568..2263
                     /gene="coaE"
                     /locus_tag="VLA60_10240"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HSF09785.1"
                     /translation="MPKFFLIYCRSQPPMLWLAMVVVGLTGGLASGKTTVARIFQSLG
                     ARIIDADELARAVVQPGKPAWRDIVKKFGPQVLAEDRTINRQALADIVFKSPKKLHVL
                     QAIIHPRVAREQAKQVKSMRHEDPHAVIIYDAALLLEAGAHRRMDHVIVVTADRATQL
                     TRACRRDGLTKAQALRRIRQQMPLCHKLDYADAVLDGTWSPTQLRHAVQSLYRTYVRE
                     ARSRTARRSLNTL"
     gene            2302..3225
                     /gene="trxB"
                     /locus_tag="VLA60_10245"
     CDS             2302..3225
                     /gene="trxB"
                     /locus_tag="VLA60_10245"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HSF09786.1"
                     /translation="MVHDVIILGSGPAGLTAAVYTARANLSPLLIDGSQPGGQLTITT
                     DVENFPGFPKGIMGPQLIQDMRAQAERFGTQFRQGHVTKVDLQVRPFRVTVDDEATLE
                     SQTLIIATGASANLLGLPSESRLMGHGVSTCATCDGFFFRGKEIAVIGGGDSAVEEAT
                     FLTKFASKVTLVHRRDKLRASKIMQDRAINNEKIAFQWNSALEEVLGDDVVKGIRVRN
                     VQTNQVEEIPVSGVFVAIGHTPNTSLFTGHIDMDEQGYIRTQPNRSTTNIPGVFASGD
                     VQDSHYRQAITAAGSGCMAAIDAERYLESLH"
     gene            3225..4427
                     /gene="thiI"
                     /locus_tag="VLA60_10250"
     CDS             3225..4427
                     /gene="thiI"
                     /locus_tag="VLA60_10250"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HSF09787.1"
                     /translation="MLHVLVHYHELALKGGNRKHFEYRLVHHLRGALKPLAHVHVEAL
                     QGRIRVSFEDEAAWPCLQEQIKRVFGIVNFSLTRAVPLAYDQSDLTVLKDAIIAHLPT
                     HPYANFRVSAKRADKRFVKTSMDVEREIGAAVADHTGKRVNLSHPDMTIFIELVPPYA
                     YYSCQRDTGPGGLPTGTGGKVICLISGGIDSPVAAYRMMKRGCKAVFVHFHGRPYLSR
                     VSEEKVRDQVALLTKYQLSSRLHLIPFGDIQSQIVINTPPPVRVVLYRRLMLRIATRI
                     AEQEEAWALTTGDSLGQVASQTPENISVINEATSLPILRPLIGMDKLEITQQAQALGS
                     FETSIEPDQDCCTLFVPKHPQTRCQLPHILKVEQALDIPKLVQQSLEGKELVTFSHAE
                     RMDRRAPL"
     gene            4624..5445
                     /locus_tag="VLA60_10255"
     CDS             4624..5445
                     /locus_tag="VLA60_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09788.1"
                     /translation="MLPPNVLFTWLYRVFVGVLLIQCLWEPINHELHLGLPSAWAEPH
                     SISEEETHHNHTAHHQDATPQTGQWEGSPEGIAYSEFNHALVGVGVILVGLSEWQTVM
                     GWQALMWVRWLLPVSLIGSGIFLLIWSDHLAWPIGSWTLAETLSGKDPEMLQHKISGI
                     LALAVGLVEGFLRAGKFTHLFWRSLLPGFALVGGLMLFRHMHGLHPGGHDIQTHHNIM
                     GTLALAGGLAWFAGEFIPRSHAALQTTSLVKFGFKILWALLVIIIGMQLLFYTES"
     gene            complement(5580..5780)
                     /locus_tag="VLA60_10260"
     CDS             complement(5580..5780)
                     /locus_tag="VLA60_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09789.1"
                     /translation="MKLSLPFFAMILKVGPASAYEEVQVLNGGAISGKVIIKGEKPRA
                     MAFNLVTILDLRNGIGGSSDYF"
     gene            complement(5876..6439)
                     /gene="nuoB"
                     /locus_tag="VLA60_10265"
     CDS             complement(5876..6439)
                     /gene="nuoB"
                     /locus_tag="VLA60_10265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HSF09790.1"
                     /translation="MFKILKKSFKTGIVTGQYPPAPAGQKFSVAPTAQKKAELFRKSL
                     TIREVDTGSCNACEMEMNALANPVYDIERLGIHIAASPRHADVLVVTGPVTVNMEGPL
                     KDVFKTTPNPKLVIALGDCAINCGIFKGSYAVTGPVDQHIPVDVRIPGCPPRPAEIIE
                     ALTELRKGVTTFTRWFKKLSGKRATDS"
     gene            complement(6588..8186)
                     /locus_tag="VLA60_10270"
     CDS             complement(6588..8186)
                     /locus_tag="VLA60_10270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSF09791.1"
                     /translation="MEPVLNPVEHLQRIFGPAINRVSTNHGIPVVHVHTNQISPITRY
                     LHANEELRGTLSLLWAVDHRPHDVRYELWYLFTLGESKDWLIVSTDLLGEERLFESIT
                     PKIHAANWYEREIRDMFGLIAQGHPDLRRLVRHNHWPKGVHPLHKNFPWNSVLQWRQP
                     PGSVDGHFIHGEGVFEVPVGPIHAGIIEPGHFRFTVAGEPIMHLDLQHFWKHRGVEKL
                     FEQQTLSGGVALAERISGDTTLGHSLAYCQAVEMLWEIHVPRRARYLRSVFLELERLH
                     NHIGDVGGICNDTAYALAHAHCSRLKERVMQLNDRLTGSRFLRGVNCVGGVAIDLSTD
                     QFSELVAELEQIEKDFSELEQIIAVNASLIDRFESTGILKKQTAWDHGVVGVVGRASG
                     IDRDLRKDRPFAAYDELSVRVAGSSHGDVRARVQVRVSEIHESIRLIREIQRMLPDGP
                     LKGDRQVVPDSGAWALSAVEGWRGEILYFLMVGEHEQIHRCKVRDPSFVNWPAIQMAV
                     IGNIIPDFPLINKSFSLSYAGNDL"
     gene            complement(8193..>9351)
                     /locus_tag="VLA60_10275"
     CDS             complement(8193..>9351)
                     /locus_tag="VLA60_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HSF09792.1"
                     /translation="SLGVQWVAVEATTLATVFLITFFKSRESLEAGWKYLILCSVGIA
                     LALFGTVLTYYSSIRVLGEENAALNLTSLLPVAAQMDGHVLKLAFIFILVGYGTKIGL
                     VPMHTWLPEAYSEAPAPVTAMLAGVLETVAVYAVLRSKSIVDQAVSPGFTGNLLILIG
                     LVSFGTAALFILIQRDYKRLFAYSSIEHMGLAMLGFGIGGPLGTFGGLWHLLNHALAK
                     SLGFFAVGNIFRRYRTRQIHEVQGLAKVQPITAIAFLVGGLALVGMPPFSLFNSELLM
                     ITSLAQKTFQFDSFHLGRFLTITVSHEIRSLGIIMVIVLLSVALFGGFIYRLTGMVWG
                     TPPAGIRQGEAWHIGQLPLALSVFALVWMGFYLPAQIETLLQRATDVVIGR"
BASE COUNT         2413 a         2611 c         2201 g         2126 t
ORIGIN      
        1 ctcccggata ttccggaaca catcgagggt ctcctcggca tgtccttcct taaacacttt
       61 ctcgtgaccc tggatgcgga gcatgctcaa ttgattctcc gtcccaagca aaaaccttaa
      121 tccaaaacgc tgtagtcccg ggatgttcag attccttgga tctctgcagc atggcgttgg
      181 tgcgagacaa aggaggggca ggttatggat ttctgcgcca acgatgtcct accacaaaga
      241 agactcttga aggttttaaa aattcgatct tctcattcac ccatgggtgc caaaaacaaa
      301 ccggagaaaa tttgtggagt gttaaaaaag actcgtccgg tcatgcactg agacttctcg
      361 aagaaaaggc cgcagacact tggacagagc cggaactgaa aaggaattta tgggttggaa
      421 gaaaccggag gcagagcccc taaatatgga aaggcaccaa cctggatcat ggaagaatcg
      481 gactgaagac gaaaatcccc gttttcagga ctgacaaaac cgggagcaaa ttgtacatcc
      541 gttttccctg cgcgttccgg agccggggtg ggtggagtcc cggtcatgac ataatccccc
      601 tggttgcctg acaaggcgtt aaaggccaac gtggtgtgac tccccggaaa aaataaaaaa
      661 cctattgcgc tctgactaat aatatttccc tgcacatcgg cttgggcatc atcatgaaaa
      721 gcagccccgc ctccgttctg agccagggta ttttgaatca accgggcttt ggactggtca
      781 gtcatcaaca cggcttcata aagacttcgg gttatcacat tctgcctgag ggtcacatca
      841 gaattgcctc cgatagccac gccatggtca ttatggtaaa tcagattgcc cgatagggtg
      901 cctgtagaat ctgcaaatgc cacacctgtt gtctcactgt caccaatcac acaatcttcc
      961 aaaagaacat cctgcacctg ttgactaaac accattccat tgacacgaat ccgtattaat
     1021 cgcacgccac tgccattaaa aatgccaact cccaatcctc catgttgaat cacggacaga
     1081 tcttgaatcg tcacattcgt tgccccatac ggccatttac caatatgaag agtcccgaca
     1141 cgcttttttc cggtcaacac taccaggtcc atgccttcac cgataatcga caaaccttcc
     1201 ttgctatgca cagtaacatc ctcggcatag gctccccgtt taatcaaaat cgtatcacct
     1261 gatgcagcct gatcaatcgc ttcttgaatc gaggtgaaat gccctgatcc atccaaggca
     1321 acctgccata ccgacgagac aacagcctca gagggcgcaa gttcctcagt attggcagac
     1381 gccaatccag gcaggacaca cagaatcagg gcacataaaa agaaaactgg tcgaatggtg
     1441 gtcggcatgt gttgaactct cacggtgttc attctcccct gtcacgctct tggccgtcca
     1501 tcggctcacc caaagaaaag gacgctcatc gagtcaataa cccaaggggt ttggcagggt
     1561 caagctggtg ccaaaatttt tcctcattta ttgtcgctcc caacccccta tgctatggtt
     1621 ggctatggtc gtcgtcgggt taactggagg attggcatca ggaaaaacga ccgtcgccag
     1681 gatatttcaa tcacttggag ccaggattat tgacgccgac gaactggcca gagccgttgt
     1741 ccaacctggg aaaccggctt ggagggatat cgtcaagaaa ttcggaccac aagtgcttgc
     1801 cgaagaccgc accatcaatc ggcaagctct cgccgatatc gtatttaaat cccctaagaa
     1861 attacacgtt ttgcaagcga tcattcatcc ccgtgtcgcc cgagaacagg ccaaacaggt
     1921 taaaagcatg cgacacgaag atccccatgc ggttatcatt tatgatgctg cgttattatt
     1981 ggaagccggt gcacacaggc gtatggatca tgtcatcgtg gttaccgccg accgggctac
     2041 tcaactcacc cgcgcctgcc gtcgtgatgg cctgaccaag gcccaagccc tccggcgaat
     2101 cagacaacaa atgccccttt gccacaaact cgactatgcc gacgctgtat tggatggaac
     2161 atggtcacct acccagttgc gccatgcggt ccagtcattg tataggacat atgtccgtga
     2221 ggcgaggtca cgaacggccc gccgcagcct caatactcta tagaccgttc acctacatgt
     2281 ctaaccgtaa ggagcccacg catggtgcat gacgtcatta ttctgggatc gggcccggct
     2341 ggattaaccg ccgccgtcta taccgctcga gccaatcttt cccccttgct cattgatggt
     2401 tcgcagcctg gcggacaatt aaccatcacc accgatgtgg aaaatttccc cggattcccc
     2461 aaaggcatca tgggacctca acttattcaa gacatgcggg cccaagccga acggttcggc
     2521 acgcaatttc gccaggggca tgtcaccaag gtagatctcc aggtgcgccc ctttcgggtc
     2581 acggttgatg acgaagccac tctggaatct caaaccctga ttattgcgac cggagcctcg
     2641 gccaatttac tgggcctgcc ctccgaaagc cgcctcatgg gtcatggcgt ctcgacatgt
     2701 gccacctgcg acgggttctt ttttcgtggc aaagagattg cggtcatcgg cggcggggat
     2761 agcgccgtgg aagaagccac atttctgacc aaattcgctt caaaagtcac cctcgttcat
     2821 cgtcgcgata aattgcgcgc atcaaaaatc atgcaggacc gggccatcaa caatgaaaag
     2881 atcgcctttc aatggaatag tgctcttgaa gaagtcctgg gtgatgacgt cgtgaaagga
     2941 atccgggtcc gcaacgtaca aaccaaccag gttgaagaaa ttccggtatc aggcgtcttc
     3001 gtagccatcg gccatacgcc gaacaccagc ctgtttaccg gccacatcga tatggacgag
     3061 caaggctaca tccgcactca gccaaatcgg tctaccacca acatccccgg cgtttttgca
     3121 tcaggtgatg tgcaggattc acattatcgc caggccatta cggctgcggg gtcaggctgc
     3181 atggcggcca tcgacgccga gcgatatcta gaatcactcc actaatgctt catgtcttag
     3241 tgcattatca tgaactcgcc ctcaagggag gcaaccggaa acatttcgaa taccggttag
     3301 tccaccatct gcgaggagct ctgaaacccc tggcacatgt gcatgtcgaa gcactccagg
     3361 gacgaatacg ggtgagcttc gaggatgagg cggcgtggcc ttgcttgcag gaacagatca
     3421 agcgcgtatt cggtatcgtc aacttttctt tgaccagagc cgtgccattg gcttacgacc
     3481 aatccgatct tacggttctc aaagacgcga tcattgcgca cctgcccact catccttatg
     3541 ccaactttcg agtcagtgcc aaacgggcag acaagcgatt cgttaaaact tcaatggacg
     3601 tggaacgaga aatcggggcc gccgtcgccg accataccgg caaacgagtc aatctttcac
     3661 accctgatat gacgattttc atcgaattag tcccgcccta cgcctactac tcctgtcaac
     3721 gggacacggg acccgggggc ctaccgacgg ggaccggcgg aaaagtcatc tgcctcattt
     3781 ccggtggcat cgattctcca gtggcagcct atcgcatgat gaaacgtggc tgtaaagccg
     3841 tctttgtgca ttttcacggt cggccgtatc tctcacgggt ttctgaggaa aaagtccgcg
     3901 atcaggtcgc gttgctcacc aagtaccaac tgtcttcccg tcttcatctc attccctttg
     3961 gtgatattca aagccaaatc gtcatcaaca cgccgccacc cgtgcgcgtc gtgctttacc
     4021 gtcgcttgat gctccgcatc gctacacgaa ttgccgagca ggaagaggca tgggccctca
     4081 cgaccgggga cagcctgggt caggtggcgt cccaaacgcc ggaaaatatt tctgtcatta
     4141 acgaagccac gtcacttccc attcttcgac cgcttatcgg gatggataag ttggaaatca
     4201 cgcaacaggc tcaagccttg ggatcgttcg agacctccat cgaacctgac caggactgct
     4261 gcaccctctt tgtcccaaaa catccacaaa cacgatgcca gctaccccat atcctcaaag
     4321 tcgagcaagc cctcgacatt cccaagctgg ttcagcaaag cctagaaggg aaggaattgg
     4381 tcaccttttc ccatgcagaa cggatggatc gaagggcacc actgtagaat gcctcttcac
     4441 acgttaaaac caccgcacac catcagacgg ttacctccat actctgaata cgcgatatga
     4501 ccaatcatat ttagcgaaat gcccgaattc tattttccag tttcagcaag acatcattct
     4561 ccaaccgtcc cacattggaa aaaccatgac caaaggaaaa tcttcagtga ggaacacgtg
     4621 agcatgctgc ctcctaatgt cctgtttacc tggctctatc gggtttttgt cggggtactg
     4681 cttattcaat gcctatggga accaataaac cacgaactgc accttggact accttccgcc
     4741 tgggccgaac cacacagcat ttcggaggaa gaaactcacc acaatcacac cgcccaccat
     4801 caggacgcta ctccgcagac cggacaatgg gaaggttccc cggaaggaat tgcgtatagc
     4861 gaatttaacc atgcgctcgt tggagtcggt gtcatccttg tgggtctcag tgaatggcaa
     4921 acagtgatgg gctggcaggc attaatgtgg gttcgttggc tgttgcctgt ttcactaatt
     4981 ggatcaggaa tcttcctgtt gatctggagc gatcatttgg catggcccat tggctcatgg
     5041 acattagccg aaaccttatc cgggaaagat cctgaaatgc tgcaacacaa aatatccggt
     5101 atcctggccc tggctgtcgg cctggtggaa gggttccttc gggctggaaa atttactcac
     5161 ctgttctggc gatcacttct gccgggtttt gcactggtcg gtggccttat gttgtttcgc
     5221 cacatgcatg gcctccatcc aggtggccac gacattcaga ctcaccataa catcatgggc
     5281 acattggcct tggcgggtgg cctagcctgg tttgccggcg agtttatacc acgttcccat
     5341 gccgcgttgc agacgacctc cctcgtcaaa tttggattca aaatcctatg ggctctcctg
     5401 gtcatcatca ttgggatgca actactcttc tacacagagt cctaatgaga tttcttcgcc
     5461 acatatcaat aaaggagaac ccactcattg atcagcctca cactccatac cgcataccaa
     5521 cacagctaat gaaggcaatt attcggcgcg gtaatccaca atgcagacca acgtcaaatt
     5581 caaaagtagt ctgatgagcc accaataccg tttcggagat caagaatcgt aaccaaatta
     5641 aaggccatcg ctctcggctt ctctccttta ataatcacct ttccggatat cgcaccccca
     5701 ttgagaacct ggacttcttc ataggccgag gccggaccta ccttaagaat catggcaaag
     5761 aagggtaatg ataatttcat gataatttcc caatcgctag agatttccaa aatccggcat
     5821 ccggccatta ctactccatt attaaaagtc gctttccggt cacagacaaa acgactcaag
     5881 aatcagttgc ccgttttccg gaaagttttt tgaaccacct ggtaaaggtc gtgacgcctt
     5941 tccgaagctc cgtcaaggcc tcaataattt cggccgggcg cggaggacat ccggggatgc
     6001 gaacatcaac cggaatatgt tgatcgaccg gaccagtcac cgcatagctt cctttaaaga
     6061 ttccacaatt gatggcacaa tcgcctaagg caatgactaa ttttggattg ggcgtggttt
     6121 taaacacatc cttcagcggg ccttccatgt tcaccgtaac cggaccagtc accaccaaga
     6181 catccgcgtg acgaggcgag gcggcaatat gaattccaag gcgttcgatg tcatagacag
     6241 gattggctaa agcattcatt tccatttcac aggcattaca cgaaccggta tccacttcac
     6301 gaatggttag cgactttcga aacagttcgg ccttcttttg cgctgttggc gcaacagaaa
     6361 atttctgccc ggcaggagca gggggatatt ggccggtcac tattcctgtt ttgaaacttt
     6421 ttttgagaat cttaaacatg gtctctcctc atttttgcgg gtcatacccc ccgaagacgt
     6481 caagtgggaa atagggctcc ctgtcgctct tccctgtcat cacgctggtt gtgttacctg
     6541 tttaccaccc ctcccccgac tcttcacgtt gcaccccttg taatgtatta caagtcattc
     6601 ccggcataag aaagactgaa gcttttatta atgagaggaa aatccggaat aatattcccg
     6661 attaccgcca tttgaatggc cggccaattg acaaacgagg ggtcccggac cttacagcga
     6721 tgaatttgtt catgttcgcc aaccatcagg aaatacaaaa tttcaccacg ccacccttca
     6781 accgcagaga gcgcccacgc tccggagtca ggaaccacct gacgatcacc cttcagcgga
     6841 ccatcaggaa gcatgcgttg aatctcgcga atgagtcgaa tggattcatg aatctcactc
     6901 actcggacct gaaccctggc tcgcacatcc ccatgtgaag aaccggccac acgtaccgaa
     6961 agctcatcat aggctgcaaa gggccgatct tttcggagat cccgatcgat gcccgaagcc
     7021 cgaccaacca ctccgacgac cccatgatcc caagcggtct gctttttgag aatccccgtg
     7081 ctctcaaacc gatcgatcaa cgaggcattg acggcgataa tttgctccag ttcggaaaaa
     7141 tctttttcaa tctgttccag ttcggcaacc agctcggaaa actgatccgt cgaaaggtca
     7201 atggctaccc ctcccacgca attcacccct cgaagaaatc tcgaacctgt cagtcgatca
     7261 ttcagttgca tcacccgttc tttcaaccga ctgcagtgag catgggctag cgcataggcc
     7321 gtgtcattgc aaatacctcc cacatcacca atgtgattgt ggaggcgctc caattccaga
     7381 aacacacttc tgagatatct ggcccgacgg gggacgtgaa tctcccataa catttctacg
     7441 gcctggcaat aagcgaggct atggccaaga gtggtgtccc ctgatattcg ttcagccaag
     7501 gccactccac cagatagcgt ctgttgttca aacaactttt ccacaccacg atgtttccaa
     7561 aaatgttgga gatcgaggtg catgataggt tctccggcca cagtaaaccg gaagtgcccg
     7621 ggttcaatga ttccggcatg aatgggaccc accggcactt cgaacacgcc ttccccatga
     7681 ataaaatgac catcgaccga tccgggaggt tggcgccatt gcaagaccga attccatgga
     7741 aaatttttgt gtaacgggtg aacacccttg ggccaatgat tgtgccggac taagcgccga
     7801 aggtccggat gcccttgggc gatcagaccg aacatatccc ggatttcgcg ctcataccag
     7861 ttggccgcat gaattttggg cgtgatggac tcaaagagcc gttcttcccc caggagatcc
     7921 gtggagacaa taagccagtc cttggattca ccgagcgtaa acaagtacca gagttcgtag
     7981 cgcacgtcat gaggacggtg atcgacagcc catagtaaag aaagggtccc tctgagttcc
     8041 tcgttggcat gaagatacct ggtaatcggt gaaatctgat tggtgtgcac atggaccaca
     8101 ggaatcccgt ggttggtgga aacacggtta atcgccggac caaatatgcg ttgcaaatgt
     8161 tcaactggat tcaataccgg ttccatgttt ggttaccttc ctatcaccac gtcggtggcc
     8221 cgttgtaaga gagtttcaat ctgagcggga agataaaacc ccatccacac caacgcgaag
     8281 accgataagg cgagtgggag ttgcccgatg tgccaggctt ctccttgcct gattcctgca
     8341 ggtggtgtgc cccataccat gccggtcagc cggtagataa atcctccaaa taacgccacc
     8401 gatagcagga cgatcaccat gatgattcca aggcttcgaa tttcatgtga taccgtaatg
     8461 gtcagaaacc gtcccagatg aaacgaatca aattgaaagg tcttttgagc cagcgaagtg
     8521 atcattaaca gttcactatt aaaaagtgaa aacggaggca ttcccaccag agccaggccc
     8581 ccgacaagaa atgcgatcgc ggtaatgggt tggactttgg ccaggccctg aacctcatga
     8641 atctgtcggg ttcgataccg gcgaaaaata ttgccgacgg caaaaaatcc cagcgatttg
     8701 gccaaggcat gattgaggag atgccaaagc cccccgaagg ttcctaatgg tccgccaata
     8761 ccaaacccca acatggccag gcccatatgc tcgatgctgg agtacgcaaa aaggcgtttg
     8821 tagtcacgtt gaatcaggat aaatagggct gccgtcccaa acgagaccaa tccgatcaag
     8881 attaagaggt tccctgtaaa ccccggggag acggcctgat ccacaatgga tttactccgg
     8941 aggacggcat acaccgcaac ggtttcaagc actccggcca gcatggcggt gaccggggcc
     9001 ggcgcttcgc tataggcctc aggcaaccag gtatgcatcg ggacaagacc aatcttcgtg
     9061 ccgtacccga ccagaataaa gatgaaagcc aacttcagca cgtgtccatc catttgagca
     9121 gccaccggca ataggctggt caggttcaac gcagcgtttt cttctccaag cacacgaata
     9181 gacgaataat acgtcaggac cgttccaaat aaagcgaggg caatgccgac agagcagagg
     9241 atgagatatt tccatccggc ctccaagctt tcccgacttt taaaaaatgt aatgaggaac
     9301 acggtcgcca gtgtcgttgc ctctacggca acccattgca caccgagact g
//
LOCUS       DATIAL010000330         4362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1244253, whole genome shotgun sequence.
ACCESSION   DATIAL010000330 DATIAL010000000
VERSION     DATIAL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244253"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VLA60_10280"
     CDS             complement(<1..712)
                     /locus_tag="VLA60_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09793.1"
                     /translation="MVSFPLSRMSFCSSIGRRLHILLALFLAGLFGMVAFTISGIQDQ
                     AVDALVIDLAGRQRMLQERHMKDVLLARQHRNANSTVTRTELVETLEALLYGRKITVS
                     WANDHAVRVPPAPTEAIKLAIHGQQELLVQFIAKSDAFLRGTQAVPAENASALELLQL
                     TDDMVAKAENLVTLYDAWASARNSRMVQWQILFGVIVGMLGVLLIKQIREANRYLEKE
                     VLERKQSEQILRESEQRHA"
     gene            complement(1025..3028)
                     /locus_tag="VLA60_10285"
     CDS             complement(1025..3028)
                     /locus_tag="VLA60_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HSF09794.1"
                     /translation="MSQLFKGMERIEEARQEMAGESFMAGLFMGNPDFNLLFPPEEPD
                     EERQIGEEYCHKIEDFLKQHVDPDDIERTAKIPEHVLKGLLDLGAFGMKIPKEYGGLG
                     FSYTNYGRVLMLMASWSNILALTVAVPQSIGIAMPILLFGNEDQKKAFLPRVARKEIS
                     AFALTEPDTGSDAANIQTHAVLNTLGTHFVVNGEKLWCTNGSLASLITLVARVPAKRI
                     FLDGKTKWVPVPEGKGAEASVHTAFILDMACPGVKIQQRCQFEGCRGIENAHMTFTDV
                     QIPVENLIGEIGKGLKYALTILNVGRAISIPALCLGMAKQAWQPTLDRANSRVTFQQP
                     LSRRQTQMRRVGQMAADLFAMEALSWLVWRMADQHAYDIRIEAAIAKIACSEKAINFL
                     KGAQTIFGGMGYEKADSKRVRGEPAFGIEQLVRDIEMYCIGEGATDILRPFVAREALN
                     PHLEQARNYLDAKGKALERIREGVHVAQWYLPRYLDLWKRKPLPSRPEIGHPKVRKHF
                     TYVERASRRLARAIFYILAIHQEAIRDDQGRQNRIESVGEDLLIMAATALWAERQYSR
                     EGDPRVWDLSDEVFHTARERIDRMIPEIIHNDDPVAASVGKRAFLGQYRFLNHGIIQR
                     GLFDYIPKVQAETLEEQGSYEKTNRENAVFTAPTDAGYRLHGR"
     gene            3293..4081
                     /locus_tag="VLA60_10290"
     CDS             3293..4081
                     /locus_tag="VLA60_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HSF09795.1"
                     /translation="MSALPRIWYTEHLADHVATVTINYPPANVLTPQGLSELESTLDD
                     LANHDQVKVIIITGTGRFFIAGADIRMLAEIGSRQKGKKLALSGQRILNKIAASPKPI
                     IAAINGICLGGGLELALCCHIRLAAEGIQLGLPEVNLGLIPGFGGTQRLVRLIGQSKA
                     TEMILTGDSLSAEDGKTCGLISEVFSDKYLMPQAIGLASRIASKSQSAVRAALQAIQR
                     GSDLKLREGLLVEATLFGELCESEDKQEGLAAFLEKRTPHFKDR"
BASE COUNT         1033 a         1176 c         1046 g         1107 t
ORIGIN      
        1 gagcatgccg ttgttcgctt tcacggagga tctgttcgga ttgtttgcgc tcaaggactt
       61 ccttctccaa gtatctgttg gcttccctga tctgctttat caacaatact cccagcatcc
      121 ccacaataac accgaacaga atttgccatt gaaccattct ggaatttctt gcgcttgccc
      181 aggcgtcgta caaggtcacc aggttttcgg ccttggccac catatcgtcg gtgagttgca
      241 gcaattccaa ggcggaagcg ttttccgcag gaacggcctg cgtccctctc aaaaacgcgt
      301 cagacttggc gataaattgg acaagcagtt cctgctgccc gtgaatggcg agtttgatcg
      361 cttcggtcgg agcaggcgga accctgacgg catgatcatt ggcccaactg accgtgatct
      421 ttctcccata aagcagggct tccaatgttt ccaccagttc tgttcttgta acggtagagt
      481 tggcattcct atgctgccgg gctagcagaa catctttcat atgccgttct tgcagcatcc
      541 gctgccggcc cgccagatct ataaccaggg catcaactgc ctgatcctgg attcctgaaa
      601 tcgtaaacgc gaccatcccg aaaagtccgg caagaaacaa ggcaagaagt atatgcaacc
      661 ggcggcctat tgaggaacaa aaggacatgc gggacagtgg aaaggacacc atgtgctctt
      721 cctaagcaaa ggtgcatgcc tagccttatg tcaacatcta tgagatagga aaagaaattc
      781 taacaggatc gtgaaaaagt ccgccagcga cctcctccac gctcaatctc ccaaccttcg
      841 catctgctcg ggattcgcac gccgtgcggc tctcctgggc gtatctttat gaacatgttg
      901 cgttgccctt aataggcaga aagcattggt tttgagatcg atctttctta tattgttaag
      961 tttttcaacg cccgcctcaa tgaccgttca atgaatatct agaaacacct cgaagagacg
     1021 cgattcatcg accatgcaac ctatatcccg catcggtcgg tgccgtgaag accgcatttt
     1081 cccgattggt cttttcatat gatccttgct cctccaaggt ttcggcttgc accttcggga
     1141 tataatcgaa aaggccccgt tgaatgatgc cgtgatttag gaaccggtat tgccccagga
     1201 acgcccgctt tccgaccgat gcggcgacgg gatcatcatt atgaataatt tccggaatca
     1261 tccggtcaat ccgctcacga gcggtatgaa atacttcatc cgacaagtcc cagacccggg
     1321 gatcaccttc ccggctatac tgccgttcag cccataacgc cgtggccgcc atgatcaaca
     1381 aatcttcccc gactgactca atcctatttt gcctgccctg atcatcacga atggcttcct
     1441 ggtgaatcgc cagaatatag aagatggctc tggccagacg gcggctcgcc cgttcgacat
     1501 atgtgaagtg tttgcggact ttgggatgac cgatttccgg acgggaaggg agtggtttcc
     1561 gtttccacag gtcaaggtat cggggaagat accattgcgc gacatgcacc ccttcccgga
     1621 tgcgttcaag ggctttgcct tttgcgtcca gataattcct ggcttgctcc aaatgcgggt
     1681 tcagggcctc acgggctaca aagggtcgca gaatatccgt ggctccctca ccgatacagt
     1741 acatttcgat atcccgaacc aattgttcaa tcccgaaggc cggctctccc cgaacccgct
     1801 tggaatcagc cttttcgtat cccatccctc caaagattgt ttgcgcacct ttgagaaaat
     1861 tgatcgcctt ttccgaacac gcgattttgg caatggcggc ttcaatacga atgtcatacg
     1921 catgttgatc ggccatacgc cacaccagcc aggacagggc ctccatggca aaaagatccg
     1981 cagccatctg tcctacgcgc ctcatttggg tttgccgccg gcttagcggc tgctgaaaag
     2041 ttacccgtga atttgcccga tccaacgtcg gttgccaggc ctgtttggcc attcccaaac
     2101 acaaagccgg aatactgatg gcccgcccga cgttcaggat ggtcaacgcg tatttcaggc
     2161 ctttcccgat ttctccaatg agattttcca ctggaatttg cacgtcggtg aatgtcatgt
     2221 gggcgttttc aatgccccga catccttcaa attggcaccg ctgctgaatc ttcacgcctg
     2281 ggcaggccat gtccaggatg aacgccgtgt ggacggaagc ctcagccccc ttcccttcgg
     2341 gcacggggac ccatttggtc tttccgtcca ggaatatccg ttttgccggc acgcgggcca
     2401 ccaatgttat caggctggcg agggagccat tcgtgcacca gagtttttcg ccgttaacca
     2461 caaaatgtgt gcccaaggta ttgaggacgg catgcgtttg aatattggcc gcatccgatc
     2521 cggtgtcggg ttccgtgagc gcaaaagccg agatttcctt tctcgcgacc cgggggagaa
     2581 atgccttttt ctgatcctcg tttccgaata gaagaatcgg catggcaata ccgatggact
     2641 gtggcaccgc aactgtaagg gccaggatat tgctccagct ggccatcagc atcagcaccc
     2701 gcccgtagtt cgtatacgag aatcccagcc cgccatattc tttgggtatt ttcatcccga
     2761 atgccccaag gtccagcagg cccttgagca catgctccgg gattttagcg gttcgttcga
     2821 tgtcgtccgg atccacatgc tgcttcagaa aatcttcaat tttatggcaa tattcttcgc
     2881 cgatttgcct ttcctcgtct ggctcttccg gcggaaacag cagattgaag tccggatttc
     2941 ccataaacag gccagccata aaactctctc cggccatttc ctggcgcgct tcttctattc
     3001 gctccatgcc tttaaaaagt tgtgacatgt ccggacctcc ttactgtgaa ttctggggga
     3061 aaccagtttt acatctaact ctagaattct cgcaggatac tcgataggtt tttcagccag
     3121 atggtctttt aaaaatttcc tttttacagt ccgcaagcaa ggaaattgaa ctcataatat
     3181 cctatcatta tgctaggata attttatatt atcaaagtgg agaccaatgc atcacggatt
     3241 cggctaaggc atgcaggcca aggatgcacg actctccaga ggagacaaga ccatgagcgc
     3301 acttcctcgc atatggtaca ccgaacacct ggcagatcat gtcgcaacgg tgacaatcaa
     3361 ctaccctccc gcaaatgtcc tcacacccca agggcttagt gaactcgaat caaccctgga
     3421 cgatctggcc aatcatgatc aagtcaaggt catcatcatc accggaacag ggcggttttt
     3481 cattgcaggg gccgatatcc ggatgttggc agaaatcgga tccaggcaaa aaggcaaaaa
     3541 actggctctt tccggccaaa gaattttaaa taaaattgcg gcttcgccga aacccattat
     3601 cgcagccatc aatggcatct gcctcggcgg aggcttggaa ttggcattgt gttgtcatat
     3661 ccgattagct gctgaaggaa ttcaactcgg gctaccggag gttaatcttg gtctgatccc
     3721 tgggttcggc gggacccaac gcctggttcg tctcatcggc caatcgaaag ctactgaaat
     3781 gatcctgacc ggcgattcgc tctcggcaga ggatggcaaa acatgtggac tgatatctga
     3841 ggttttctct gataaatatt tgatgcctca ggctatagga ctcgccagtc ggattgcctc
     3901 aaaaagtcaa agtgctgttc gagcagccct tcaggctatt caaaggggat cggatttgaa
     3961 acttcgtgag ggattgctgg tagaagcaac cttatttgga gaactttgcg aatccgaaga
     4021 taaacaagaa gggctggcgg cctttttaga aaagcgcact ccgcatttca aggaccgctg
     4081 ataccccaag tgttttagcg aatctgtcag gaaacgaggg gggatggatc agttggtttg
     4141 gaaagattcc tgctcaatct aatcaccact tttcctacat acacaataat ttggtggtag
     4201 tgagaggctc ctggatttca tgaaagggag gcacacgatc aattaacctg atcttttctt
     4261 ggtatcgttc gacttcaaag gtagtccggt tacaccattg ttttgacatg cctgttgctc
     4321 ttcaatagcc cattcactcg ttattggtgt ggggagcatc cc
//
LOCUS       DATIAL010000331         2658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_706614, whole genome shotgun sequence.
ACCESSION   DATIAL010000331 DATIAL010000000
VERSION     DATIAL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706614"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VLA60_10295"
     CDS             complement(<1..544)
                     /locus_tag="VLA60_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09796.1"
                     /translation="MPLSSENSGMVKQPIRQNRLHKVLHKNLRIPPWVIRPFYRALKI
                     TSSPTQYRLRKRLAREIVANTKPRMTIPDKAGYRLFGPDDIEGTDRIVRYCETVYQQS
                     REAFPPEYFQKHPHKKFLFPILEGAEFCRHPELLKFMVSRPILDAAAVYLGTVPKLTG
                     ARLCWSPENETALSSQLFHFD"
     gene            874..1617
                     /locus_tag="VLA60_10300"
     CDS             874..1617
                     /locus_tag="VLA60_10300"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSF09797.1"
                     /translation="MGTLVLLRHGESLWNREKRFTGWTDIGLSPKGVIEAQQAAQILK
                     GKRMMFDLCFSSVLSRAHETATIVLKNMDLTSVPVRKSWRLNERHYGALQGMTWWEAS
                     TQFGAKQVLIWQRHFSAVPPSLSPQDSRFPGLDPLYADLNPQDLPLTESLQDTQRRLI
                     PFWEEDIAPLLKEGKRILLVAHNNSIRSLLQFLLHLDDASIKQITIRTGEPLVCKFDA
                     KGNLISYSYMRWKPKLKDCTQKLLKYCLP"
     gene            1642..2355
                     /locus_tag="VLA60_10305"
     CDS             1642..2355
                     /locus_tag="VLA60_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSF09798.1"
                     /translation="MTYLNFSALEAISAQSFQEAKPYPWMNPPDLLTDAGYQGLVETL
                     PNVSMFQKRFGVKRAHGQQSHDRYTLEYRPGLPLSPHWNQFLAELQGKPYSNFLKRLF
                     GVKSLELNFHWHYTPNGCSVSPHCDAKHKLGSHIFYFNTKEDWDPAWGGETVILDDQG
                     KFSRKSAPSFEDFPQSLASTAIGNFSLLFQRLDKSWHGVRPVQCPEGHMRKVFIVVIN
                     RLSLVDRIQRVFGKSPKGY"
     gene            complement(2393..>2658)
                     /locus_tag="VLA60_10310"
     CDS             complement(2393..>2658)
                     /locus_tag="VLA60_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09799.1"
                     /translation="HLLCAVDVEGLVSEYLIGMGAAKALVRWLNPFKNEFFRLLATKG
                     APDIQAYYGFIELCFLAQWTKRAFFERDWKAIKPDYFEGYRGL"
BASE COUNT          712 a          653 c          589 g          704 t
ORIGIN      
        1 aatcgaagtg aaagagttgg ctggagagag ccgtttcatt ttcaggtgac caacaaagcc
       61 gggctccggt caacttcggg accgttccca gatagacggc tgcggcatca agaattgggc
      121 gggacaccat aaacttgagt aattccggat ggcggcagaa ttcggcacct tcaagaatcg
      181 gaaacagaaa ttttttatga ggatgttttt gaaaatattc cgggggaaaa gcctctcgtg
      241 attgctgata cactgtttca caatacctga caatgcgatc ggttccttca atatcgtcag
      301 gcccaaaaag gcggtatccg gccttatctg gaatggtcat ccggggcttc gtgttcgcaa
      361 caatttctct ggccaatcgc tttcgcaaac gatattgagt gggactactg gtaattttca
      421 gcgcacgata aaatggtcga atcacccacg ggggaattct cagattttta tgcagtacct
      481 tatgcagccg attctgacga atcggctgct tgaccattcc cgaattttca gaggatagag
      541 gcataaatac gattccgtgc ggaaaaatgg agaatttggc tgtatagctt gggcttcatt
      601 catcttccat ggatgccaat gaacgatccc tgcacatggg catccaggta aagcgactca
      661 tgattcctga aaatacgctt tctgtcaatc attcacattg agctccccgt gaaacagaat
      721 gccccatttt gaaaatcctt attcattctc gagaaaacct atggtattct taggattgca
      781 tccgaagatt gtaaattcaa tgccttttga ctttaattct gcgaagcgat tacctgtccg
      841 aaacatcaag gaacaaactt tccttataac aaaatgggaa ctctcgttct tttacgccat
      901 ggagaaagcc tgtggaaccg cgaaaaacgg tttacaggct ggaccgatat cgggttaagc
      961 ccaaaaggtg tcattgaagc tcagcaggca gcccaaattc ttaaggggaa aagaatgatg
     1021 tttgacctgt gtttctcatc ggttttaagc cgcgcgcatg aaacggcaac cattgtactg
     1081 aagaacatgg atttaacctc tgtcccggtg cggaaaagtt ggcgactgaa tgaacgtcat
     1141 tacggtgctc tccaaggaat gacctggtgg gaagcatcaa cacagtttgg tgcgaaacaa
     1201 gtcctgatat ggcagcgaca cttttctgcg gttccacctt ccctctctcc tcaggattct
     1261 cgatttcccg gcctggatcc cttatatgca gatttgaatc ctcaggacct tcccttgaca
     1321 gaaagcctcc aggacaccca acgccggctc attcctttct gggaggaaga cattgctccc
     1381 ctgcttaaag agggaaaacg gattctgctg gttgcacaca acaatagtat ccgaagtttg
     1441 ctacaatttc ttcttcatct cgatgatgcc agcatcaaac aaatcacgat tagaaccgga
     1501 gaacccttgg tctgtaaatt tgacgccaaa ggcaatctta tcagttactc ctatatgaga
     1561 tggaagccga aattgaagga ttgcacacaa aagttgttaa aatactgcct cccctaagct
     1621 catcattgat cgtttgtggt tatgacgtat ctaaactttt ccgccctaga agctatttca
     1681 gcccaatcct ttcaggaagc caagccctat ccgtggatga atcccccgga tctcctgacg
     1741 gatgcaggtt atcaaggcct ggttgagacc cttccgaatg tctccatgtt tcaaaagcgt
     1801 tttggggtca aacgggctca cgggcaacaa agccatgatc gctacacatt agagtatcgc
     1861 ccgggcttac ctctctctcc tcattggaat caatttctag ccgagcttca gggaaaaccc
     1921 tattcgaatt ttttaaaacg attatttggg gtcaagtccc ttgaattgaa tttccattgg
     1981 cactataccc ccaatggatg ctctgtttct ccgcattgtg atgccaaaca taaacttgga
     2041 tctcacattt tctattttaa cacgaaagag gattgggacc ccgcctgggg tggcgagacc
     2101 gttattctcg acgatcaagg gaaatttagc cgaaaaagcg ctcccagctt tgaagatttt
     2161 ccacaatcgt tggcttctac cgccattggg aatttcagtc tgttatttca acgattggat
     2221 aaatcctggc atggggtccg ccccgttcaa tgcccggaag ggcacatgcg aaaggtgttc
     2281 attgtcgtca tcaatcggct ctcactggtg gaccgaatcc agcgggtctt tggaaaaagc
     2341 cccaaggggt actgattttc tcaatacgtt ctatcgctca ttgccgatac tcctacagtc
     2401 cccgataccc ttcgaaataa tcgggcttta tggctttcca atctctttca aaaaatgccc
     2461 gtttcgtcca ctgggcaaga aaacacaatt cgataaatcc ataataggct tgaatatccg
     2521 gcgcgccctt agtcgccaac aatcggaaaa actcgttttt gaatggattc agccatcgca
     2581 ccaaggcttt ggcagcaccc atgcctatga gatactcact gacgaggcct tccacatcga
     2641 cagcacacag caaatggg
//
LOCUS       DATIAL010000332        10200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_277996, whole genome shotgun sequence.
ACCESSION   DATIAL010000332 DATIAL010000000
VERSION     DATIAL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277996"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VLA60_10315"
     CDS             <1..1262
                     /locus_tag="VLA60_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF09800.1"
                     /translation="ASPEGVVTTLKAIPGFYGNPVWDAALAGVLVLAIMYLTPGRIAK
                     VAPPSLLALLVVTPLAYAFLPDAPTIGEVPRGFPTPLMPTITWETLQIVLESAAILAV
                     LGAIDSLLTSLVCDNMTRTQHDPDRELIGQGIGNMVAGVFGGLPGAGATMRSVANIRT
                     GGRTPISGVLHSIILLAVLLGLGPLAEKIPLAVLGGILFKVGIDIIDWRFLRHILQAP
                     RIDVVIMTVVLLTTVLVDLITAVAVGMIFASLFFVKRMADLELANLHIVTNPTPSTPL
                     LPEEATILQQANGKILLIHVDGPMSFGSAKTMVRRLETVPGFNTFSSVVLDLSKVPAI
                     DGTAALAVEDMLNIVKAHDQHLFFVGMQPHVTKALDGLGVLVQIRPGHRFASRLEALQ
                     KASLVEGTPSKDPSRPSAPNKNLPATP"
     gene            1354..2652
                     /locus_tag="VLA60_10320"
     CDS             1354..2652
                     /locus_tag="VLA60_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HSF09801.1"
                     /translation="MATSILILTYAVIIAERFNRAIVALLGAATVIGTGVLTQDQAIQ
                     GIDFNTIGLLTGMMILVGITKECGIFQFLAIKAAKAAKGSPWGILVMLSIVTAVLSAF
                     LDNVTTVLLVAPVTLLIADELKINPYPLLFAEINASNTGGTATLIGDPPNIMIGSATS
                     LTFNDFVIHLAPIAIVAFSATLIPLWFMYGKTMFASLEARARIMAFNEYEAIRDVGLL
                     KHCLLVFSLVILGFILAHSIGMEPATIALGGAALLLLLVTFSLDNEQAGHKVHSMFGQ
                     VEWITIFFFCGLFIIVTGVEHTGLLQSIADWTLSLTAGNFTVTTLAILWVSAILSAIV
                     DNIPFVATMIPMIKSMLPVFQQAGIPLEQAETLWWALSLGACLGGNGTLVGASANLIV
                     AGIAERNGVPFRFLPFLKVAFPLMILQVLISTVYIWMRYL"
     gene            2649..3098
                     /locus_tag="VLA60_10325"
     CDS             2649..3098
                     /locus_tag="VLA60_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF09802.1"
                     /translation="MNALDIMIRQPHTIPPQCTIGKALRQMADLRLQAFPVVDEKKML
                     LGTLNFWQILEWAMPSYISQGDLPDVRFAPDLAQFHERLGELKPNPVTQVMNPHPPCA
                     RPDDSVLACAALIMKTPKTVYLLPVVEGDRQLVGIISAWDIIKEIAG"
     gene            complement(3154..3615)
                     /locus_tag="VLA60_10330"
     CDS             complement(3154..3615)
                     /locus_tag="VLA60_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09803.1"
                     /translation="MKSKGKGRGGVWWGAGTCLAIAVCVAGSGIAGSQEPSFSTPRQV
                     VEQWFEVYPGNMERAAELTTTAFREGISKEEWIAIRGPYLRDLQLKYVRAKIAHEKMV
                     GEEAHVIVHAHIVTLMGDQPQDELYVLLKNPEGRWVIDQVEVYTESFNTGP"
     gene            3831..4907
                     /locus_tag="VLA60_10335"
     CDS             3831..4907
                     /locus_tag="VLA60_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HSF09804.1"
                     /translation="MAPLTNKPGIHQGEKAHPQEHLTRLLAIIFTGIVCFLIFAILWP
                     NQPSPLDIVIRALGIALGGAWLYRFLTRHIRHRAMIRQAPFPTEWEKILAQNVPFYQA
                     LSLRDQNRFREEVHIFLREKRITGINTSVDDTVRVLVAASAIIPIFGFPGWEWEHIRE
                     ILIYPTSFDEGYQMGSSQEHPISGMVGTGAMNRMMILSKPDLLQGFKQSSHGHNVGIH
                     EFTHLLDKSDGTVDGVPEIALPGRAIRPWLNLVQNEMNKIRKGHSDINPYGLTNEAEF
                     FAVVSEYFFEKPVKMKQKHPALYAMLKQIFRQDPQSRITGALKAMLRPKQASLKHPAP
                     CPCGSGKPFEQCCPTNNVNSVFDK"
     gene            5112..5975
                     /locus_tag="VLA60_10340"
     CDS             5112..5975
                     /locus_tag="VLA60_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF09805.1"
                     /translation="MRVLLATDGSEQSSHATKAVMSLTSVSTLTILHVIDLPRLTFSM
                     LGPEIAFDLSKVAGEALRKDGEQVLTRTKALLSDKVKSLETRLEEGSPAELILSVAQE
                     EHPDLILMGARGRGQVEELFLGSVSQRVLTHAVCPVLIINRPLMEIKKILIAIQSSAD
                     VEKVHRFLTKHPFQPQTEITLFSVVPLPRSLFRGGVSAPEDKIEQALESTEAFLEQAV
                     SQLKGCYDSVKGYVGLGAPANTILEQASITEPDLLVMGMHHPSTITRFVLGTVSHTVL
                     HQATRSILVIR"
     gene            complement(5997..6482)
                     /locus_tag="VLA60_10345"
     CDS             complement(5997..6482)
                     /locus_tag="VLA60_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HSF09806.1"
                     /translation="MIYEVEGDILLSEAVAVAHGVAPNDNFAHGLALALRERWPAMYK
                     DFRHYCQVSNPKSGELWSWAGAGNVRIINLLTQEAPPSHGANPGKASVENINHCLKAL
                     RKTIESEKFSSVALPRLATGVGGLDWKDVKPLIEKHLGDLPIPVYVYSTYHPGVKAKE
                     R"
     gene            6565..7494
                     /locus_tag="VLA60_10350"
     CDS             6565..7494
                     /locus_tag="VLA60_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09807.1"
                     /translation="MPAILACTFKTTLTPTEIHKRAIEISVPLQQVFPEMRNTGQASG
                     WLKGQRSKGISLADRLRSGYHRFWYEASQALRWSRGPFREDLKGPMTNLTPTQQHRTK
                     ELAKSYRSRFETSLTPETALLNYSYLDILDRARHALGWPVPLYQRVCDLGSANFAYAA
                     ALHTFFHPNRLTGVEVDGHRLYCNGRSRIDYASGHIQDLSQTEYVVADFRRFTGQADI
                     ITAWYPFVTPKPLLAWRLPLSLFNPAAVFAQVANNLIIGGTFVMINHSGAEAEVARGI
                     AEDVGLVCAGHYLHQAPLRPRTADPVLTIWHHG"
     gene            complement(7662..8897)
                     /locus_tag="VLA60_10355"
     CDS             complement(7662..8897)
                     /locus_tag="VLA60_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF09808.1"
                     /translation="MGSFFVLTVFTAFRFLNRNRLRSGLTTVGIIIGVGSVIAMVSIG
                     EGAKFAIQAQVASMGTNMILILPGATSVGGVRGGSGGAVTLTVNDALDLQKKVPTLHE
                     TGWAKRDVMQIVYGNQNVNVPVNGISPTYLTIRDWSFTSGGPFTEGEMETTSRVALLG
                     QTVVDNLFVPGEDPVGKMIRIKNVPFRIIGVLAPKGQSPTGNDQDDIIFIPFTTAERR
                     VLGTQFLGSVGALFASTERPEDLPAAVEDIQRVLRDRHRLQPDQPDDFTIRTQVDIGK
                     VQEGTGETLTIMMFSVASISLLVGGIGIMNILLVSVTERTREIGIRMAVGAKRRHILI
                     QFLAEAMTLSMVGGLLGVGFGILGAQATTIVAGWPTIISFEAIMVAFLFSAAVGLIFG
                     IYPANKAARLNPIDALRYE"
     gene            complement(8906..9616)
                     /locus_tag="VLA60_10360"
     CDS             complement(8906..9616)
                     /locus_tag="VLA60_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09809.1"
                     /translation="MSQLIACTNLSKTYRLGDIDVEAIRGINLAIQPGEFIAIMGSSG
                     SGKSTLMNILGCLDVPTAGSYCLNGRDIAHAEPDELAEIRSRTIGFVFQNFSLIPKTS
                     ALENVQLPLFYQGLSLREQRSRAQSALARVGLANREHHYPTQLSGGQQQRVAIARALV
                     SAPSLLLADEPTGNLDTQSSQEIMDIFQSLNHTDGLTILVVTHEIDIAAYASRQIQMR
                     DGCILSDQTSIGMGMRVS"
     gene            complement(9842..>10200)
                     /locus_tag="VLA60_10365"
     CDS             complement(9842..>10200)
                     /locus_tag="VLA60_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09810.1"
                     /translation="AFRFFPSQSASVGQRMGESGSDVVKPLPGASPPGLHAWGGSHGR
                     KTVWKLTDSGDPTSVLLQPGISDGSKTEVVESELREQDKIIVGIAIPKGERKVNQLPP
                     GFGSGQKSTSSRDKGL"
BASE COUNT         2456 a         2839 c         2418 g         2487 t
ORIGIN      
        1 ctgccagccc cgagggtgtc gtcacgacac tcaaggcaat tcctgggttt tatggaaacc
       61 cagtgtggga tgccgccctt gccggggtcc ttgttttggc cattatgtat ttgactccgg
      121 gtaggattgc gaaggtggcc cctccttctt tattggccct cctcgtggtt accccactcg
      181 cctatgcttt tcttcctgat gcccctacca tcggagaggt tccacgggga ttccccaccc
      241 ctctcatgcc aacgatcact tgggaaacct tgcaaatcgt gctggaatca gccgccatcc
      301 tggccgtatt gggagcgatc gacagcctgt taaccagcct ggtctgcgat aatatgaccc
      361 ggacccaaca tgacccggac cgcgagctta ttggtcaagg catagggaac atggtcgccg
      421 gtgtgtttgg aggtctccca ggtgccggag ccaccatgcg gtcggtagcc aatatccgaa
      481 ccggaggacg gacaccgatc tcgggcgtac ttcactccat aattttgttg gcagttcttt
      541 tgggacttgg ccctttggcg gagaaaatcc ccttagccgt cctcggggga attttgttca
      601 aagttggtat tgatattatt gactggcggt tcttacgaca catcctgcaa gccccccgca
      661 tcgatgtcgt catcatgacc gtggtgttac tgaccaccgt gctggtggat ctcatcaccg
      721 ccgtggccgt aggaatgatc tttgccagcc tgttttttgt caaacgcatg gcggatttgg
      781 aactagctaa tctccacatc gtgaccaacc ccactcccag cacccctctt ttaccggaag
      841 aagccacgat tctgcagcaa gccaacggga aaattctctt gattcatgtc gatggcccca
      901 tgagctttgg atccgccaaa accatggtcc gccgcctgga aaccgtcccg ggcttcaata
      961 cgttttctag cgttgttttg gatttatcca aagtcccggc cattgacggg acagcagcat
     1021 tggcggtgga agatatgttg aatatcgtca aggcccacga ccaacatctt ttcttcgtgg
     1081 gcatgcagcc tcacgtgacc aaggcattgg atggcttggg agtgctggtg cagattcggc
     1141 ctggccatcg ctttgccagc cgtttggagg cactgcagaa agcctccttg gtagaaggaa
     1201 ctccctctaa ggatccatca cgaccttcgg caccaaacaa aaatcttcct gcgactccct
     1261 gaacaggcct gggtttttga ccctgaatat tgggctttta aaaatatgca cgagacacct
     1321 atccagacca tctttgggtt ctccccactt tgggtggcta cttcaatttt aatattgacc
     1381 tatgccgtca ttatcgctga acggtttaac cgggcgattg tagcgcttct tggggccgct
     1441 accgttattg gaacaggagt cctcacacag gaccaagcga ttcaaggcat tgatttcaat
     1501 accatcggcc tactcaccgg catgatgatc ctggtcggaa ttaccaaaga atgcggcatc
     1561 tttcaatttc tcgcgatcaa agccgcgaag gcggccaagg gcagtccgtg gggcatcctg
     1621 gttatgcttt caattgtgac cgcagtgctc tctgcatttt tggataatgt cacgacggtg
     1681 ctattagtcg ctcccgttac cttgcttatt gctgacgagc tgaaaatcaa tccgtaccct
     1741 ctgctcttcg cagagattaa tgcctccaat acaggtggca ccgctacgct gatcggtgat
     1801 ccccccaaca ttatgatcgg ctctgccacc agtttgacat ttaatgattt tgtgattcac
     1861 ctggctccca tcgccattgt agcctttagt gccacactca tccctctttg gtttatgtat
     1921 ggaaaaacca tgttcgcatc acttgaagcc cgagcccgaa ttatggcttt taacgaatat
     1981 gaagccattc gggatgttgg tctgttgaaa cactgtcttc tggtttttag cctggtcatt
     2041 ctcggattta tcttagccca ttcgataggc atggaacccg ccaccattgc tctgggtggt
     2101 gccgccctgc tcctattact cgtcacattt tccttagaca acgaacaggc agggcataaa
     2161 gttcatagca tgttcggaca agtggaatgg attaccattt tctttttctg cggattgttc
     2221 attatcgtca ccggtgtgga acataccggc cttttacagt ccattgcaga ttggaccttg
     2281 agtctaaccg caggaaattt caccgtgaca accctggcca ttctttgggt ttcggctatt
     2341 ctttccgcta ttgtggataa catacccttt gtggccacga tgattcctat gatcaaaagc
     2401 atgctcccgg tctttcaaca ggctggtatt ccacttgaac aggccgaaac cctctggtgg
     2461 gcattatcgt tgggtgcgtg cttaggagga aatgggacac tggtgggagc ctccgccaat
     2521 ttgattgtgg cggggattgc ggaacggaat ggcgtgccat ttcgatttct gccatttttg
     2581 aaagtcgcct ttcccctgat gatcctacaa gttttgatca gcaccgttta catttggatg
     2641 aggtatttat gaatgctctg gacatcatga tccgccagcc acataccatc ccaccacaat
     2701 gcacgatcgg gaaagccctt cgccaaatgg cggatctgcg actccaggcc tttccggtcg
     2761 tggatgagaa aaagatgtta ctgggaacac tgaatttctg gcaaattctt gaatgggcca
     2821 tgccttccta catttctcaa ggggatcttc ccgatgttcg ttttgctccg gaccttgccc
     2881 agttccacga acggcttgga gaacttaaac ccaacccggt cactcaggtg atgaaccccc
     2941 atcctccctg tgcccggccg gatgactccg tattggcctg cgcggctttg atcatgaaaa
     3001 ctccgaaaac ggtctattta ctgcccgtgg tggaaggaga tcgtcaactg gtgggaatta
     3061 tttctgcctg ggacatcatt aaagaaatcg cggggtagga gacaaggcgg gacatggctg
     3121 catcatcctt tgcagctcct gactccttcc gtttcaagga cccgtattaa acgattccgt
     3181 atatacttcc acctgatcga ttacccacct gccctcagga ttcttcagca ggacatacaa
     3241 ctcatcctgc ggctgatcac ccatcagcgt gacaatgtga gcatggacaa tcacatgggc
     3301 ttcttctccc accatcttct catgggctat cttggcacgc acatatttca actgtaggtc
     3361 ccgaagatac ggacctcgga tggcaatcca ttcttctttg gatattcctt ccctaaaggc
     3421 cgttgtcgtc agctcagcgg cccgttccat atttcccggg tagacttcaa accactgttc
     3481 caccacctgg cgaggtgtgg aaaaagatgg ctcttgagac cccgctatcc cgctaccggc
     3541 cacgcacacg gcaatcgcga ggcaggtccc tgccccccac cagacacccc ctcgcccttt
     3601 gccttttgac ttcatggtag agtccccctt atctggcgaa ttccggataa cttgaaagag
     3661 tccttggcca tatcaatgga ctaaacgggt agggtccagg agaaaacacg aaatgctgtt
     3721 ccaactttgc aaggtcattt cttgtcccct ccccttacag atccttttgt tggcatcgag
     3781 agtgccacga atattggtaa tgtaaagaaa tttttcagga ttgactcgac atggcaccat
     3841 tgacaaacaa gcctggaatc catcaggggg aaaaagctca ccctcaggaa catctcaccc
     3901 gtctcctagc catcatcttt accggaattg tgtgtttcct catcttcgcc atcctctggc
     3961 caaatcaacc gtctccactc gacatcgtca tacgggcttt gggcatcgcg ctgggcggcg
     4021 cctggttgta ccgcttcctg actcgtcata ttcggcaccg ggcgatgatt cgtcaagccc
     4081 cgtttcctac cgaatgggaa aaaattctgg cacagaatgt cccattctat caggcgcttt
     4141 ctctccgcga ccaaaaccgt tttcgagaag aagtccatat tttccttcgt gaaaagcgaa
     4201 tcaccggcat caatacttcg gtagatgaca ccgtccgggt attggttgca gcaagtgcca
     4261 tcattcccat atttggattt ccaggctggg aatgggaaca tatcagggaa atcctgattt
     4321 atccgacctc cttcgatgag gggtatcaaa tgggatcatc ccaagagcac cctatctcag
     4381 gcatggttgg aacaggggcg atgaaccgca tgatgatttt gtccaaaccg gatttattac
     4441 aaggtttcaa acaatcttca cacggacata atgtgggtat tcatgagttt acccatttat
     4501 tggataaatc tgatggcacc gtagacgggg ttcctgagat tgccttgccc ggccgggcca
     4561 ttagaccctg gctcaacctg gtacagaatg aaatgaacaa aatccgaaag gggcattcag
     4621 acatcaatcc ctatgggctc acaaacgagg cggaattttt cgcggtggtc tcagaatatt
     4681 tttttgaaaa acccgtgaag atgaaacaga aacaccctgc actctatgca atgttgaaac
     4741 agatctttcg ccaggatccc caatcacgaa ttaccggtgc cctcaaggct atgctccgcc
     4801 caaaacaggc ttctctgaaa catcctgctc cctgcccatg tggaagcgga aagccctttg
     4861 aacagtgctg cccaaccaat aatgtaaatt ccgtcttcga caaatgacgc cgcttgagaa
     4921 aacaggaaca atgccaaact atggaagctc aaccaagctt tgagcctttt ggggacatct
     4981 cggcttgagt ccctgactca cgataaaaaa catgcagcgc tgaccaaaga cccaaacccc
     5041 caaaacagac cgtctaggaa agcaggacca tgcacgatca caatgaaaag atgaaagagg
     5101 agaaatgacc aatgcgagta ctcctggcca ctgatggatc ggaacaatcc tcccacgcca
     5161 caaaggcagt catgagctta acctcagtca gtaccctgac cattctgcac gttatagatc
     5221 tccctcggtt aaccttttct atgctcgggc ctgaaattgc ctttgacctg tcgaaagtcg
     5281 caggggaagc actccgcaaa gacggtgaac aggttttaac acgcacgaag gctctcctgt
     5341 cagacaaggt gaagtcgctg gaaacccgat tggaagaagg ctcaccggcc gagctcatat
     5401 tatcggtggc ccaggaggaa catcctgatc tcattctgat gggcgctcgt ggaagagggc
     5461 aggtagagga gctttttctg gggagtgttt cacaacgggt tctgacccac gcagtatgtc
     5521 cagtcctgat catcaacagg ccattaatgg aaatcaagaa aattctgatt gccatccaaa
     5581 gctccgctga tgttgaaaag gtgcaccggt ttttaacgaa acaccccttt caaccacaga
     5641 cagagatcac cctcttttcg gtggtaccgc ttccacgttc cctctttcgg ggaggcgtct
     5701 ctgcaccgga ggacaaaatc gaacaggctt tagagagcac cgaagccttc cttgagcagg
     5761 ccgtcagtca actcaagggc tgttacgatt cagtcaaggg atatgtggga ttaggagccc
     5821 cggcgaatac gatattggaa caggcctcaa tcacggaacc agatctcctg gtgatgggaa
     5881 tgcaccatcc ttcaacgatc actcggtttg tgttgggcac cgtgtcccat accgtgctcc
     5941 accaggccac gcgatccatt ctcgtgattc gataacagga cgggctgcgc gcggcgttag
     6001 cgttctttgg cttttactcc tggatgatag gtggaataga catacacggg aatgggtaaa
     6061 tctccaagat gcttttcaat taatggtttc acatccttcc aatccaatcc ccccactccg
     6121 gtcgcaagcc gtggtagcgc cacactgctg aacttttctg attcaatcgt tttccgaagc
     6181 gctttcaaac agtgattgat attttcaacc gatgcttttc ccggattggc cccatgactc
     6241 ggtggagctt cctgtgtcaa gagattgata atccgcacgt ttccggcccc ggcccaactc
     6301 cataactcac cggacttcgg attggaaacc tggcaataat gacgaaagtc tttgtacatc
     6361 gcgggccacc gctcccgtaa cgcgagcgcc aatccatgtg cgaaattgtc attgggggct
     6421 acaccatggg caacagccac ggcctcactt aataaaatgt caccttccac ttcataaatc
     6481 atgcgcttcc ctccttgtca gatatcataa tccctttgtc tggtaaaaga gaaataacca
     6541 tagcacaagg cgctcagacg attcatgccg gccattctcg catgtacatt taagaccact
     6601 ctcaccccta ccgaaataca taaaagagca atcgagatat ccgtccctct tcaacaggtc
     6661 tttccagaaa tgcgaaatac ggggcaagcc tccggctggc tcaaggggca acgctcaaaa
     6721 gggatatccc tggccgaccg gctccgatcg ggctatcatc gattctggta tgaagcctcg
     6781 caagctctgc gatggtctcg tggaccattc cgcgaagatc tcaaggggcc aatgaccaat
     6841 ctgacgccga cccaacaaca cagaaccaaa gagctggcca agtcatatcg ctcgcgcttc
     6901 gaaacctcgc ttactcccga aactgctctt ctgaattatt catatctgga catattggat
     6961 cgtgcccgac atgcgcttgg ttggcctgtt cccctctatc aacgggtctg tgatctcggg
     7021 agtgccaatt tcgcgtatgc tgcggccctt cacacattct tccatccaaa ccgcctgaca
     7081 ggcgtcgagg tcgatgggca tcgtctgtat tgtaacgggc gaagccgcat cgattatgcc
     7141 agcgggcaca ttcaagattt gtcgcaaacc gagtatgtgg ttgccgactt tcgccggttc
     7201 accggacagg ccgatattat tacggcctgg tatccatttg tcactcccaa acccttactg
     7261 gcctggcgat taccgcttag cttattcaat ccggccgctg tattcgccca agttgccaac
     7321 aatctcatta ttggcggaac cttcgtcatg atcaatcaca gtggcgccga agcagaggtg
     7381 gcgcgaggca tcgcggaaga tgtcggcctc gtttgtgccg gccactatct tcaccaagct
     7441 cccctccgtc cgcgaacagc agacccggtg ttaaccatct ggcatcatgg ataacctgac
     7501 aggaacagtt acgtgcgttt tcagaaggcg gttatgggaa gggatttcga atattcgacg
     7561 ggagaggggg aaggcctggc ttacattttc ccactgggga ttttctgaac agtttacgtc
     7621 ccttttaaag ggggccttca ggcttgtccc ccttctgcgg attattcgta gcgcaaggca
     7681 tcgatgggat ttaatcgcgc agctttgttt gcagggtaaa taccaaagat cagcccgaca
     7741 gcggcagaaa agagaaacgc gaccataatg gcctcaaagg aaataatcgt gggccatccg
     7801 gccactatgg tcgtcgcctg tgcgccaaga ataccgaagc ccacacccag caaccctccc
     7861 accatactga gcgtcatcgc ttccgctaaa aattgaatca agatatggcg acgcttggct
     7921 ccaacggcca tacgaatacc aatctcgcgc gtgcgttccg tgacagacac cagcagaata
     7981 ttcatgatcc cgattccccc gaccaataac gagatcgagg ccacggaaaa catcatgatt
     8041 gtcagggttt cccccgtccc ctcctgaact ttcccaatat ccacctgggt gcgaatcgta
     8101 aaatcatccg gttgatccgg ctgcagacga tgacggtccc gaagcactcg ttgaatatcc
     8161 tcgacggctg cggggagatc ttcggggcgc tccgtcgacg caaacaatgc cccgactgat
     8221 ccaagaaatt gggtgccgag aacccggcgt tccgccgtgg taaaggggat gaagataata
     8281 tcgtcctgat cgttgccggt cggagattgg cctttgggtg ccaggacgcc aatgatacga
     8341 aacggaacgt tcttgattcg aatcatcttt ccgaccggat cctcaccggg aacaaataga
     8401 ttgtcgacca ccgtttggcc caagagggcc acacggctag tggtctccat ctctccttcg
     8461 gtaaacggtc cgccactggt gaacgaccaa tcacgaatgg tcagataggt cggagaaatc
     8521 ccattcaccg gaacattgac attctgattt ccgtaaacaa tttgcatgac atcccgcttg
     8581 gcccagccgg tttcatgcag tgtgggcact tttttttgca aatcgagtgc atcattgacg
     8641 gtcagggtca ctgcaccacc cgaaccacca cggacgcccc ccacactcgt cgctcccggc
     8701 aatattagaa tcatattcgt gcccatgctg gccacttgcg cctgtatcgc aaatttcgct
     8761 ccttcgccaa tactcaccat ggcgatgact gaacctacac cgataataat gccaaccgta
     8821 gtgagaccgg atcgcaaacg atttcggttg agaaagcgaa aagctgtgaa caccgtcaag
     8881 acaaaaaaac ttcccatcca gttccttatg atactctcat acccatacca attgaggttt
     8941 gatcgctcag gatacagccg tccctcatct gaatctggcg tgaagcataa gccgcgatat
     9001 cgatttcatg cgtcaccacc agaatagtca acccatcggt gtgattgagg ctctgaaata
     9061 tatccataat ttcctgactt gattgcgtat ccagattccc ggttggctcg tccgccagga
     9121 gaagggaagg agctgacacc agcgcgcggg caatggccac tcgctgttgt tgcccaccag
     9181 acaactgtgt ggggtaatga tgttcccgat ttgccaaccc cacgcgcgcc aaagccgact
     9241 gagcacggga tcgttgttca cgaagagaaa gcccctgata gaatagcgga agctgaacat
     9301 tttctaatgc gctggtttta ggaatcagac tgaaattctg aaaaacaaac ccgatggtcc
     9361 gactacgaat ctccgccaat tcatcgggtt cagcatgggc aatatcccgt ccattcaaac
     9421 aataactgcc tgcagtcggc acatccaggc aacccaagat attcatcaat gtggatttac
     9481 cagatccgga cgagcccata atcgcaatga attcgcctgg ttggatagcc agatttattc
     9541 cccgaatggc ctctacatca atatcgccca agcgataggt tttggagaga ttggtacagg
     9601 caatcagttg actcatcgtc atctttggcg aagaacattg ctagagaatg gaggccaagg
     9661 ttgccagacc atttttatgg caacccccgg aaaatcccct atgaccaatt tcccccttgc
     9721 aaggagtgca aaaatgttca gcgtctggac aagggccaga gcagtgaaaa agacacgcgc
     9781 gcccagctcc agagaccaag accgattgag gaacaacatc ggaacatttc cccttcgtcg
     9841 gctacagccc cttgtctcgt gaactcgtag acttttgtcc tgacccaaag cccgggggaa
     9901 gctgattgac ttttcgctcc cctttcggaa tggcgatccc gacaataatc ttgtcctgct
     9961 cccgaagttc actttcaacc acttccgtct tgctaccatc cgaaatgcca ggctgaagaa
    10021 gcacggaagt tggatcgccc gaatccgtca acttccaaac cgtcttccgg ccatggcttc
    10081 ccccccaggc atgaagtcct ggaggtgatg ccccgggcag tggtttcacg acatcagatc
    10141 cggactcacc cattctttgc ccgacagacg cggattggga agggaaaaag cggaaggccg
//
LOCUS       DATIAL010000333        11237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_814038, whole genome shotgun sequence.
ACCESSION   DATIAL010000333 DATIAL010000000
VERSION     DATIAL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11237
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814038"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1069
                     /locus_tag="VLA60_10370"
     CDS             287..1069
                     /locus_tag="VLA60_10370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HSF09811.1"
                     /translation="MKPDLREVEMVFDLFVPSVGGGGGYPVRKSRNVRDGYARGWGLQ
                     CGQLRESVRKDALYQEAYRLASGRTVVSEDNRMNIFLILKYFLPYIPFGHIVEFGSYK
                     GGNALFMAYVVDQLYPGRKVFALDTFTGMPATESAIDAHGAGDFQDVDLQELREFADR
                     EGLRNVEFIKGLFQETGPSVVDKIGSIVLAHIDCDIYSAVAFSYDLVKPFMVPGGYLV
                     FDDATTSSCLGATEAVEELVIRRDRMHSEQIFPHFVFRSHGV"
     gene            1103..1840
                     /locus_tag="VLA60_10375"
     CDS             1103..1840
                     /locus_tag="VLA60_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF09812.1"
                     /translation="MKECSKSIARRLSEPNFINRFFVGKGLDIGGHPDPLGLYQELFC
                     RMEDVRTWDLADGNAQFLEGVPDETFDFVHSSHCLEHLHDPVVGLRNWFRVLRPGGHL
                     VITVPDEDLYEQGQFPSTFNADHKWTFTIFKMQSWSRKSLNVLDLVRDLGAAAEPVKI
                     EQLISTYRFNLPRYDQTLSSVGECGIEFVIRKRPVAEVDAGGRWCRSSDQPRDEVRIH
                     LNQYRDDLRTLKHFNREKPPFHNDQPL"
     gene            1856..2884
                     /locus_tag="VLA60_10380"
     CDS             1856..2884
                     /locus_tag="VLA60_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HSF09813.1"
                     /translation="MIIRARAPLRLGLAGGGTDVSPYCDLYGGCVLNATIDRYAYAVL
                     EVLTSHKVQFCSADRQHFSEYQLGETIPCNGGLDLHRAVYLYMVEQHNEGRPLPVKVT
                     TYCDAPVGSGLGSSSTLVVAMLKAFAELLNVPLDDYALAHLAFKIERVDCGLQGGRQD
                     QYAATFGGFNFIEFYADNRVIVNPLRIKNWIICELEASLVLFFTGVSRSSAKIISDQS
                     TNVEAGTDLAVSAMHAMKREACTMKECLLKGDFTGLVESMREGWVSKKRSAKSVSNAH
                     IDEIYEAAVKAGALAGKVSGAGGGGFMMFFVPPEKRMEVIFALKKFSGQVSNCHFTKY
                     GMQGWKIE"
     gene            3137..3853
                     /locus_tag="VLA60_10385"
     CDS             3137..3853
                     /locus_tag="VLA60_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSF09814.1"
                     /translation="MAQVGIKKAIVLAGGRGERLQPVVPDLPKPMAPVRGRPFLEYIL
                     DRLVDAGLTEVTISVGYKAEVIEEHFGQAYRSLQIRYSRERYPLGTGGAMALALKGDE
                     SSPTLVLNADTIVDIDYPALMAWYAQTPTQVAIVLRHVPDVSRYGAVVLLGERIVEFQ
                     EKGQEGPGLVNAGVYVVGPEIFSQYGSGEHFSFETDILQPYCRELQPRAFVTTGYFID
                     IGTPSDYERAQKEMIFTRIN"
     gene            3886..4053
                     /locus_tag="VLA60_10390"
     CDS             3886..4053
                     /locus_tag="VLA60_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09815.1"
                     /translation="MGFEKEFEQFWKKGYLIVPDLFDQEEMHILKNIIVNHEGMKQRT
                     EGLMDKVSQGK"
     gene            4075..4689
                     /locus_tag="VLA60_10395"
     CDS             4075..4689
                     /locus_tag="VLA60_10395"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HSF09816.1"
                     /translation="MWNDTAGNDVFAKATRRASIFDRLEYFFNDSIYVYHNKITLKYP
                     GMVGFRYHQDYAYWYGMGNLYPDMATVLSAVDPMTRENGCLKILEGSHKMGRIDHVFH
                     DGVSDSGVCQDRLAVIETRLPEVYVELKRGDVVMFHCNTLHGSNDNASPHSRIALIGC
                     YNTKSNNPYKSAGGHPFYQVQERVLGKITEEDSSSLPDFDLQFS"
     gene            4815..5051
                     /locus_tag="VLA60_10400"
     CDS             4815..5051
                     /locus_tag="VLA60_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09817.1"
                     /translation="MKHLGSFLAICITLIVFGFENSAQAYLDPGTGSMVLQLLLGGIA
                     GAVVILKLYWRRFVGLFRGNAREESEHSSSEDQK"
     gene            5102..6451
                     /locus_tag="VLA60_10405"
     CDS             5102..6451
                     /locus_tag="VLA60_10405"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF09818.1"
                     /translation="MYQLDGRIFRTVNDCFSQDFNFVTSTGLFQKLATEGLLLPFEVV
                     SSDVLGHLDPKPRYVLEAPRLPFMSYPYEWSFPALKAAALLHLRVHLKSLAVGVTLSD
                     ASAYNVQFQGNQPVFIDHLSFRRYQSGEMWVGHRQFCEQFLNPLLLRAFFGISHNAWY
                     RGTQEGITTGEIRKLLKWRHCLKWNVLTHVVLQDLFQKTSTKNTKSLQDHSLAKASFP
                     LSSFKAMLEKMFHWISQLTPLNKGASTWGDYDKTCSYSSTEIQSKKQFILEFSQTVQP
                     TLIWDFGCNTGEYSEAALEGGARNVVGFDFDQGALDGSYHRAAERRLSFQALFMDAAN
                     PSPNQGWNGHERESLQARASADAILALALVHHLAIARNIPLVQVVNWLITLAPEGVIE
                     FVPKNDPMVQELLSLRQDIFPDYTAEHFMALLKEKAVIIKNETISKSGRVLVWFKRM"
     gene            6651..9233
                     /locus_tag="VLA60_10410"
     CDS             6651..9233
                     /locus_tag="VLA60_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HSF09819.1"
                     /translation="MPGIRMVLMEFLHLFALAGFAVAQPLYDLLGQNPEFFVSYKAGP
                     ELIIGMVFVLSIGMPLGLVLLELAVWPVGERVRNSLHLVFVFGLAFLTVLPPVQRLVN
                     GSDLLIVGFALLIGFLFSVFYVHWQAIRLFLTVLSPVVVAFPLWFLLFTPVGRLVMPD
                     AIEAQADIGINNPVPVVLIVLDEFNITALLDAERRIDPVRFPNFAALASQSQWYPNAA
                     AAYVETAVAVSAILTGRKPRGDIRLNPTATDHPQNLFTILGDRYALNVVESVTRLCPH
                     TLCKGEDAAAGSSLRYKSFFADLAVIFLHIIAPPHLGKQLPPLHAQWTGFGEELLKKG
                     ILGQNESRIHSDLAIGEKKSRESQITSFLSQIKQTSSPVLHFLHVVLPHTKYEYLESG
                     HQYVSKSGHIPAGWIDGPGWGGRWIGKESLILTAYYQYLQQIGYVDQFLGKLRRSLER
                     EELFDKALIIVTADHGVSFQRGLSMREVQKGNESDILKVPMFVKLPGQREGSIHERLV
                     SGIDVLPTIADVLGLKIPKAIDGFSMLNNEIPPRDEIDFLGVGKIKAQDLEGFPRLKW
                     QVDHFGEHTSLDRLVPRGPASNLIGQEFGDLDIGHSSSLGYINENPGQLDQVNLESRN
                     LPALFSGQIINTSRRSLPIAISLNGRIWATTQASLWDGKRNYFSVLFPPSAFQSGENQ
                     VRVFLIGDHDTKLTPIPLAIQGSLSDEQTMIRLHHEASGQSKLVFPGEREIVVDVGEK
                     HLTGNLDRVLLSEESIVVEGWAADLEKNQPAEEVFIFTNEKLTARAEVGISRQDVVEA
                     HQQKSLFHSGFRIKIPVKVLKPNPSDIRFIVVSFSNRAWEFSFSPEQLDFIRSTLEEK
                     NFSQ"
     gene            9675..10883
                     /locus_tag="VLA60_10415"
     CDS             9675..10883
                     /locus_tag="VLA60_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09820.1"
                     /translation="MKQNLALVVGSTIITIFIMEALLRGMGIPSTLKSGWGWENSAGR
                     KLSKYADLTTNQFGYRGQSIHYQTEDYVVLLVGDSQVEAYANRPEHMPEQFLQEFLTT
                     QLNKPVKVFSLASSGWGQDQQLLALQEYFNLYRADLVLLWATPGNDFWENAFPDRSAT
                     PQAGPLKPTFRLMEGELYGPFFTSESYLHNSAIAQLIETVTANLQKETLEQRILRRWL
                     QGMPSSDRAHIAENDHVCEGLTVINQVEFFNTIFNLNNEIGYTIRSGEDFLNSRSHFS
                     PFMLNPSRRDEYLVAITESLLQHVKEEVERHHSKFFVFYPVREDFDKRGMQMVRCVMD
                     AQGDIFRVSLDYASRLKDVTSSEDLVFFDLPGGNEIVVSPSDRHLNDSGNKLAMKKLS
                     LMLIERSLFN"
     gene            10948..>11237
                     /locus_tag="VLA60_10420"
     CDS             10948..>11237
                     /locus_tag="VLA60_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09821.1"
                     /translation="MLIEDANTEGITAFFCHGENVDGKPTDASLNFYDLSKKAAICPL
                     LNHWDEPGSLPLYRQQWRRMGKDGKGGMPQKQKKCYSCLQPRDFNDWMGWLSG"
BASE COUNT         2765 a         2356 c         2940 g         3176 t
ORIGIN      
        1 gaccctgata ttgagcatgc cgtatggttt atgaagcgag tcgtcactga gccgcattat
       61 gcgcaaacaa ttggtcaaca tgcggcggac tttatcaaaa cccatcatag ctcacaggcg
      121 gtggggacca aatatcgagg tcgacttgag gctttaaacc tgctagatga actgtgaagg
      181 agaagaccct tctccttcat aaggggaagg gatcgggatt ggcacgggtc ctcatgggtg
      241 ccgtgaaatg actgtgaatg atgtgtgacc cagggatgac agaggaatga agcctgatct
      301 gagagaagtg gaaatggtct ttgatttatt tgtcccttcc gtcgggggtg ggggaggcta
      361 tcccgtccgg aagtcccgta atgttcggga tggctatgcc agaggatggg gattgcaatg
      421 cggccaattg cgtgagtcgg tgcgtaagga tgcgttatat caagaggcat acaggttggc
      481 ttctggccgg actgtggtct ctgaagacaa ccggatgaat atttttctga tcctgaaata
      541 ttttcttcct tatattccct ttggccacat tgtcgaattt ggatcgtata aaggtgggaa
      601 tgcgctgttt atggcctatg tggtcgatca actgtatcct ggcagaaaag tctttgcgct
      661 ggatactttt acgggaatgc cggcaaccga atcggcaatc gatgcccatg gagcaggaga
      721 ttttcaagac gtcgatttgc aggagttgcg ggaatttgct gatcgggagg gtttgagaaa
      781 tgttgagttt atcaaaggat tgttccagga aaccggcccc tctgtcgtcg acaagattgg
      841 aagcattgtc ttggcccata tcgactgtga tatttattcc gcggtggctt tttcatatga
      901 tctggtgaag cctttcatgg ttccaggtgg gtatctggtg tttgatgatg cgaccacctc
      961 aagctgttta ggagcgactg aagcggtaga agaattggtg attcgtcgcg accgtatgca
     1021 tagtgagcaa attttccccc actttgtctt tcggtctcat ggagtgtgat acctaacctt
     1081 tccattattc gtcaacgtaa ttatgaaaga atgtagcaag tccattgctc ggcgattatc
     1141 cgagccaaac ttcattaacc gttttttcgt cgggaagggt ctggacatcg gtggccatcc
     1201 cgatcccttg ggtttatatc aggaattgtt ctgtcggatg gaggatgtgc gaacatggga
     1261 cctggctgat ggcaatgcgc agtttcttga gggggtgccg gatgagacct tcgactttgt
     1321 gcatagcagt cattgcctgg agcatttgca tgatcctgtt gtaggactcc ggaattggtt
     1381 tagggtgttg cggccaggcg gccatctcgt cataaccgtc ccggatgaag atttatatga
     1441 acagggccag tttccgagca catttaatgc cgatcataaa tggacgttta ccattttcaa
     1501 gatgcagtca tggagtcgaa agtctctgaa tgttctggat ttggtacgtg accttggtgc
     1561 tgctgccgag ccggtcaaaa ttgaacagct tatttcgacg tatcggttta atcttcccag
     1621 atacgaccag accttgagtt cggttgggga atgcgggatc gaatttgtca ttcgcaagcg
     1681 tcctgttgcg gaagtggacg caggtgggcg ttggtgtcgg tcctcagacc aacctcgaga
     1741 cgaagtgcgg attcacctga accagtaccg ggacgatctt cgaacactta aacatttcaa
     1801 ccgggaaaaa ccaccgtttc acaatgacca gcccctttga tatctggagg gttcgatgat
     1861 catccgcgcg cgggcgcctc ttcggcttgg cctggcggga ggcgggacag atgtgtcgcc
     1921 ctattgtgat ttgtatggcg gttgtgtgct caatgccacg attgatcgat atgcctatgc
     1981 ggtgctggag gtgttaacga gccataaggt gcagttttgt tctgctgatc gccagcattt
     2041 tagtgagtat caattgggag aaaccattcc atgcaatgga gggctcgatt tacatcgagc
     2101 cgtctatctg tacatggttg agcagcataa tgaaggacgg ccgttgcctg tgaaagttac
     2161 cacttactgc gatgctcctg taggatctgg gttaggatcc tcctcgactc tggtcgtggc
     2221 gatgcttaag gcctttgcgg aattgctgaa cgtcccgctt gacgattacg cgttggctca
     2281 tttggcgttt aagattgagc gagtcgattg tggtctgcag ggcggaagac aggatcaata
     2341 cgccgcgaca tttgggggat ttaactttat tgagttttat gcagacaatc gggtgatcgt
     2401 caatcccttg cgtatcaaaa actggatcat ctgtgagctg gaggcttcat tagtgctttt
     2461 ttttactggt gtgtccagat cttcggcaaa gatcatcagt gatcagagta ccaatgtcga
     2521 ggctggcact gacttggcag tttctgccat gcatgccatg aaacgtgagg cctgtacgat
     2581 gaaggagtgt ctgttgaaag gggatttcac tggactggtt gaatccatgc gcgagggatg
     2641 ggtcagcaaa aaacgttcag ccaagagtgt gtccaacgcg catatcgatg aaatctatga
     2701 agcggcagtc aaagcgggag ccttggccgg taaggtgtcg ggggctggcg ggggaggatt
     2761 catgatgttt ttcgtcccac cggaaaaacg catggaagta atttttgcgt taaaaaaatt
     2821 ctccggccaa gtcagtaact gtcatttcac caaatacggg atgcaaggtt ggaagattga
     2881 ataaattgaa tcgtgtggaa gaaatcagac cgcggttgac ctttttacat ggttgtttga
     2941 aaaaatcgaa tgtgttggtg aggtaaattt agaacctatt tagtccgaac aaccaaggat
     3001 agggatggta aggttttggt ctgatgcgta ttcaactgac ttcttccgta agaagctgag
     3061 tcgctgatat gtttgcgcca gggttatcct tctctggcat gtgtttgatt gatgtctgaa
     3121 gacaggagtg attcatatgg ctcaagtagg aatcaaaaag gccattgttc tggccggggg
     3181 acgtggcgaa aggcttcagc cagtggtccc agatcttcct aagcccatgg cgcctgtgag
     3241 aggccggcca tttttggagt atattctcga tagacttgtc gatgctgggc tgaccgaagt
     3301 taccatatca gttgggtata aggcggaggt cattgaagag cattttggtc aggcttaccg
     3361 ctcacttcaa atcagatatt caagggaacg ctatccttta ggaacaggag gagcgatggc
     3421 cttagcgcta aaaggtgatg aatcttctcc gactttggtc cttaatgccg acacgatcgt
     3481 tgacattgat tatccggccc tgatggcctg gtatgcacag accccaacgc aggtcgcgat
     3541 cgtacttcgt catgtcccgg atgtcagtcg gtatggtgcc gtcgtgctcc tgggtgaacg
     3601 gatcgtagag tttcaagaaa agggtcagga aggtccaggc ctagtaaacg ctggtgtgta
     3661 cgttgttgga cctgaaatct tttcccaata tggaagcggg gagcactttt cctttgagac
     3721 ggatatcctt cagccgtatt gtcgggagct gcagccacgc gcattcgtca ccacaggata
     3781 ttttattgat atcggcacgc caagcgacta tgagcgcgct cagaaggaaa tgatttttac
     3841 gcggattaat tgaacggcat cggttcggca aacatggaga gtgggatggg atttgagaaa
     3901 gagttcgaac agttttggaa gaagggatac ctgattgttc ctgatctgtt cgaccaggaa
     3961 gagatgcata ttctgaagaa tatcatcgtg aatcatgagg ggatgaaaca gcgcactgaa
     4021 ggtctcatgg ataaagtttc tcagggaaaa tgaccctctt ttgaaaccat ttttgtgtgg
     4081 aatgatacag cgggaaatga tgtcttcgcc aaagccacac gacgtgcgtc gattttcgat
     4141 cgattggaat atttttttaa cgattctata tacgtgtatc ataataaaat taccttgaaa
     4201 tatcccggta tggtcggatt ccgctatcat caggattatg cctattggta tggaatgggg
     4261 aatttgtatc cggatatggc tacggttctc agtgccgttg acccgatgac cagggaaaac
     4321 gggtgtttga aaattctcga ggggtctcat aagatgggca ggattgacca tgtgtttcat
     4381 gatggggtgt ctgacagcgg tgtttgtcag gaccgcctgg ctgtcattga aacgcgtttg
     4441 ccggaagtat acgtagaatt gaagcgagga gatgtcgtga tgtttcattg taataccctg
     4501 catggttcca atgataacgc gtctccccat tccagaatcg ccttaatcgg ttgttataat
     4561 actaaatcaa ataatccgta taagtcggca gggggccatc ccttctatca ggttcaagaa
     4621 cgagtgttgg gaaaaataac ggaagaggat tcaagcagcc ttcctgattt tgatttacaa
     4681 ttttcctgat gtcacttcag agtttgtccc ggaggataat agccctgtta ctgtttgacg
     4741 ataattaatg tgccaataag gctcgaacct gaaatgtcca aggtattggg aaaggctagg
     4801 gagggaatag caaaatgaaa catttaggga gttttcttgc aatttgtatt actctaatag
     4861 tgttcggatt cgaaaattcg gcacaggctt accttgatcc cggtaccgga agtatggtgt
     4921 tgcaactctt gttgggcggc attgccggcg ccgtggtgat tctcaaattg tactggaggc
     4981 gatttgtcgg actatttcgt ggaaatgctc gagaagaatc cgaacattcc tcttccgaag
     5041 atcagaagta atgggtggtg tccaacccct tccaggatcc ttccgagatc ccagtggaaa
     5101 cgtgtatcag cttgatgggc gtatctttag gacggtcaat gattgctttt ctcaggattt
     5161 caattttgtc acttccacgg gattattcca aaagcttgcg accgaaggct tgctgcttcc
     5221 gtttgaggtc gtgtcgtcag acgttctggg gcacttagat ccaaagcctc ggtatgtttt
     5281 ggaagcaccc aggctcccat ttatgtccta tccctatgaa tggtcatttc cggcactgaa
     5341 ggccgccgca ctcctgcatt tacgagtcca tttgaaatca ttggcagtcg gtgtcacgct
     5401 ttccgatgcc tcggcttaca atgttcaatt tcagggaaat cagcctgttt ttattgatca
     5461 cctctccttt aggcgctacc aatctggtga aatgtgggtt gggcatcgcc aattttgcga
     5521 acaattcctg aaccctctct tacttcgcgc attttttggc atctctcaca acgcctggta
     5581 tcggggaacg caggaaggaa ttacaactgg agagatccga aagctactca agtggcgaca
     5641 ctgcttgaag tggaatgtct tgacccacgt cgttttgcaa gatcttttcc aaaaaacttc
     5701 cactaaaaat acgaaaagcc ttcaggatca ttcattagcc aaggcctcgt tccccctttc
     5761 ttctttcaag gcgatgctgg aaaaaatgtt tcattggatt agccaattga cacctctgaa
     5821 taagggtgca agcacttggg gggattacga taagacctgt agttattctt caacggagat
     5881 ccaatctaag aaacaattca ttcttgaatt ttcccaaacg gtccagccca cactgatatg
     5941 ggattttggg tgtaatacgg gtgaatattc ggaggcggcg ttggaaggcg gggcacggaa
     6001 cgtcgtgggg tttgactttg atcagggagc gttggatggc agttaccatc gagccgccga
     6061 gaggcgtctc tcttttcaag ctctctttat ggatgcggct aatccgagtc caaaccaggg
     6121 gtggaatggt cacgagcggg aaagtctgca ggcacgggct tcagcggatg ccatcctcgc
     6181 cttggctctt gttcatcact tagctattgc caggaatatt ccgttggttc aggttgttaa
     6241 ttggctgatc acgcttgcgc cagagggggt catcgaattt gtcccgaaaa atgatcctat
     6301 ggtgcaagaa ttgttaagtc tgcgtcagga tattttccct gattacaccg ccgaacattt
     6361 catggcgctt ctgaaagaaa aggcggtgat cattaagaat gaaaccatct caaaaagcgg
     6421 acgagtgttg gtgtggttta aacgcatgta attcagaggc atataacctg gtcaaattta
     6481 cttaagaatt tgaaccatca tgaaaaatct gcggtgtcgt tgccatacgc cttgaatatt
     6541 tccgcaccct ttcctctaaa gacacttgtt atcccaacca taaacatctg aatttgaccc
     6601 aagctctgtc atcagggacc ccaatgccca cttcctaaga tgagttaaat atgcctggta
     6661 ttcgtatggt ccttatggaa tttttgcatc tttttgccct cgctggcttt gccgttgccc
     6721 agccgctcta tgatcttctt gggcagaacc ccgagttttt tgtttcttat aaggccggtc
     6781 cagagcttat catcggtatg gtcttcgtcc tttcgatcgg gatgccatta ggcctcgtgc
     6841 ttcttgaact tgccgtttgg ccggttggtg agcgagttcg gaatagcctg catttggtat
     6901 ttgtctttgg cctggcgttc ctcacagtcc tgcctccggt gcagcgattg gtgaatggat
     6961 ctgatctctt gattgtcggg tttgcattgc tgattggttt tcttttttcg gtcttttatg
     7021 ttcattggca ggcgatccgg ctgtttctta cggtgctttc acccgtcgtg gtggcatttc
     7081 cactgtggtt tttgttgttt acccctgtgg gtcggcttgt catgccggat gccattgaag
     7141 cacaggctga cattgggatt aacaatcctg tcccggttgt ccttattgtg ctggatgaat
     7201 tcaacatcac cgcgttattg gacgctgaac gtcgaatcga tccggtgcgt ttcccgaact
     7261 ttgccgcctt ggcctcccaa agtcagtggt atcccaatgc agctgcagcc tatgtagaaa
     7321 ccgcagttgc ggtgtctgcc attttgacgg gtcggaaacc gagaggtgat attcggctta
     7381 acccgacggc aacagatcat ccgcaaaatc tgttcacaat cttgggagac cgttatgctc
     7441 ttaatgtcgt ggagtctgtg acaagacttt gtccccatac actatgcaag ggagaggatg
     7501 ctgctgctgg ttcgtccctt cgttacaagt ccttttttgc agatctggca gtgatattct
     7561 tacatattat tgccccgcca catttaggaa aacagttgcc cccgctccac gctcaatgga
     7621 ccggtttcgg tgaggaattg ttgaagaaag ggattcttgg gcagaacgag agtcgtatcc
     7681 attccgatct tgccattggg gaaaaaaaat caagagaatc tcaaatcact tctttccttt
     7741 ctcaaatcaa gcaaacctca agccctgtcc tccatttcct tcatgtcgtc ctacctcata
     7801 cgaaatatga gtatcttgag tccggtcatc aatatgtaag caaaagcgga catattcctg
     7861 ccggttggat tgatggccct ggttgggggg ggaggtggat tggaaaagaa tccttgattc
     7921 tcacggcgta ctaccaatac cttcagcaaa ttggatatgt tgatcagttc ctcgggaaat
     7981 tgcgacgttc acttgaaagg gaagagcttt tcgataaggc gctcattatt gttacggcag
     8041 atcatggtgt ttcctttcag cgagggctat ccatgcgaga ggtacaaaag ggaaatgaga
     8101 gcgacattct gaaggttccc atgttcgtca agcttcctgg gcaacgtgag ggaagcattc
     8161 atgagcggct tgtcagcgga attgatgtgt tgccaaccat tgccgatgta cttggtttaa
     8221 agatccccaa ggctatagat gggttttcca tgttgaacaa tgaaattcca ccgagggacg
     8281 agatagattt tctcggagta ggaaaaatta aagcccagga cctggaggga tttcctcgtc
     8341 ttaagtggca ggtcgatcat tttggagagc acacctcact cgaccgactt gtgccaagag
     8401 ggccggcttc aaacttgatt ggacaagaat ttggcgattt ggatattggt cactcgtctt
     8461 cactaggata tatcaatgaa aaccctggtc aattagatca ggtgaacctt gaaagcagaa
     8521 acctccctgc cctttttagc ggacaaatta ttaacacgag tcgccgaagc ttaccgattg
     8581 ccatttctct taatggccga atttgggcta ctacccaggc atccttatgg gatgggaaac
     8641 gtaactattt ttctgtttta tttcctcctt cagctttcca gtcaggcgag aaccaagtga
     8701 gggtgtttct gattggagat catgatacaa aattaactcc catacccttg gcaattcagg
     8761 ggtctttgag tgatgagcag acaatgatcc gtttgcatca cgaagcctcg gggcaatcaa
     8821 aactggtgtt tcccggtgaa cgggagatcg tggttgatgt gggtgaaaaa catctgactg
     8881 ggaatctgga tcgtgttctt ctttcagagg agtcaattgt tgtggaggga tgggctgctg
     8941 atcttgaaaa gaatcaaccg gctgaagagg ttttcatttt cacaaacgaa aaattaacgg
     9001 ctagggcaga agttggaatc tcgaggcaag atgtcgtgga ggctcatcaa cagaagtcat
     9061 tgttccacag tgggttcagg attaaaatcc cggtgaaagt tctcaagcct aatccaagtg
     9121 atatcagatt cattgttgtc tcgttcagca accgagcatg ggaattctcc ttttctcccg
     9181 aacaattgga ttttattcgc tcgacgcttg aggaaaagaa tttcagccaa tgaccaggcc
     9241 aataagggca aatccagtca ttagtcgtga caaacccaag gtatcgctta accggaactc
     9301 catgaaagcg ttggattagg tgaggaacag ttccagcttt ttatttagtg cggcgcttgg
     9361 cacctggacg agccttttta ggattttcaa ggaagcttct tgacgtcgga ctgagtgggg
     9421 catgttttgt gggatcgttt ggattgtatc tcatggaaga tatgctcggg ttggtctgtc
     9481 tgattttgga aaccaaaagt ttgttcccgc tgcgaatgtt actcattaaa cggggccaga
     9541 ggattgaacc ggttgcccca acatatttcc taaagcatcc ttttgatgat gattgaatcg
     9601 aacatgatta ttagaagtta acgttaagtt cccgtctcct tgggggcgag cttgcggtgg
     9661 aagcctggaa gcagatgaaa cagaacttgg cccttgttgt tggatcgaca ataattacga
     9721 ttttcataat ggaggcctta ctgcgtggta tgggaattcc atctaccctg aagtcagggt
     9781 gggggtggga aaattccgcg ggtagaaaat tatcaaaata tgctgattta acaaccaatc
     9841 aatttggata tcgaggacaa agcattcact atcagaccga ggattatgtg gtattgcttg
     9901 tgggcgacag ccaagtcgaa gcgtatgcca atcgcccgga acacatgcct gaacagtttc
     9961 ttcaggaatt tctaacgact cagttgaata aaccagtcaa agtgttttcc ctggcttctt
    10021 cagggtgggg tcaggatcaa cagctactcg ccttacagga gtatttcaac ttgtataggg
    10081 cagacttggt tttgttgtgg gcaaccccag gaaatgattt ttgggagaat gcctttcctg
    10141 atagaagtgc gactccgcag gcgggaccct tgaagccaac ctttaggctt atggagggtg
    10201 agctctatgg cccctttttt acatcagaat cttacctgca taatagtgca attgcccaat
    10261 taatagagac ggtgacggca aatttacaga aagaaacatt agagcaacgc attcttcgtc
    10321 gttggcttca aggaatgcca tcgtccgata gagctcacat agccgaaaat gaccatgtgt
    10381 gtgagggact gacagttatt aatcaggtag agttttttaa tacgatattt aatctcaata
    10441 atgaaatcgg ttacaccata agatctgggg aagattttct gaacagcaga agccactttt
    10501 ctccttttat gctgaatcca tccaggcgag atgaatatct ggttgcgatt actgaaagtt
    10561 tattgcagca tgtaaaagag gaagttgaaa ggcaccattc aaaattcttt gttttctatc
    10621 ctgttcgaga agattttgat aagcgaggta tgcaaatggt taggtgtgtg atggatgctc
    10681 aaggagatat atttagagtt tcattggact atgcaagccg gttgaaggac gtcacctcct
    10741 ctgaagactt agttttcttt gatttgcctg gtgggaacga aattgtcgtt tcaccgagtg
    10801 ataggcattt aaatgattct ggaaataaat tagctatgaa gaaactgagt ctgatgttga
    10861 tagagcgttc cctatttaat tgataattat ttttcccctt tgaggctatg gtgatagttt
    10921 ggttccgctg tctttcgaac aataatcgtg ttaattgagg atgccaatac cgaaggcata
    10981 acggcattct tctgtcatgg agaaaatgtc gacgggaaac ccacggatgc ctcactcaac
    11041 ttctacgact tatccaagaa agcggccatc tgcccactgc tgaaccattg ggacgaaccc
    11101 ggcagtctcc cactttatcg gcagcaatgg cgccggatgg gcaaggatgg gaagggaggc
    11161 atgccgcaga agcaaaaaaa gtgttattca tgtctacagc ctagagattt taatgactgg
    11221 atgggatggt tgtcagg
//
LOCUS       DATIAL010000334         5204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_491701, whole genome shotgun sequence.
ACCESSION   DATIAL010000334 DATIAL010000000
VERSION     DATIAL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491701"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..752
                     /locus_tag="VLA60_10425"
     CDS             186..752
                     /locus_tag="VLA60_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09822.1"
                     /translation="MNTRQFGILVMTTLLAGVVGGALSNLVFINAAVSAEESEQPKKV
                     VTAEVFRLVTKDGQPRATLLLWNGELPALTFADETCHNRVFLGVFNEAQPVLILNDKG
                     CKQRAALDLQPDGLPSLTLRDKNDVPRARLRVLLDGSPVLTLFNQNGQIAWSPPLSSS
                     EPWRSTRERYPLGRDQDIPEEQTDVSNT"
     gene            902..1336
                     /locus_tag="VLA60_10430"
     CDS             902..1336
                     /locus_tag="VLA60_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF09823.1"
                     /translation="MAIQGVPASGFKTVGQIVPTNELKFGRKLNALAAAVELLSQHTP
                     GGPVVDEKNHFIGFISEFDVLRALEAGKDLNKVTVEDVMKEDHIAIADTTSIKDAVHL
                     MEKHRLLSLPVERNGEVMYTVTRHDLLRAWIGLGLGIESGNE"
     gene            1505..1795
                     /locus_tag="VLA60_10435"
     CDS             1505..1795
                     /locus_tag="VLA60_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09824.1"
                     /translation="MNSESNSYVTVRVFEASDPCEWELPGPNGTAIEGWHLLSVGCGH
                     SGRYSLQVTFDQFHCVNAEGQKVKVHLSGVSAQKLEKEPSLPSARTHRLPQD"
     gene            2100..2573
                     /locus_tag="VLA60_10440"
     CDS             2100..2573
                     /locus_tag="VLA60_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09825.1"
                     /translation="MRALAYVLIGVLGGMGVMSLSQKGGPSPTTMLAPVSYAMMSAEP
                     ASEIAEEKPWTGADLLEMAQLYDQKADELQSEAVRLEQRAISLAEKPHMDPKGFTRTG
                     WMLIASSRWKSAKELREMAAMHRSEGQRLLALEKNGEVPTEDLDKEGNASQKHGT"
     gene            3097..4647
                     /locus_tag="VLA60_10445"
     CDS             3097..4647
                     /locus_tag="VLA60_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF09826.1"
                     /translation="MIKKPDWKELFTMTWKDVQRDFLASVVVFLVALPLCMGIAIASG
                     APPVKGIITGIVGGLVVGLLAGCPLQVSGPAAGLTVIVFDVIQRFGLGKLGIVVLVAG
                     GIQVLAGFLQLGQWFRAVSPAVIQGMLAGIGVLILASQFHVMIGDTPKGSGLENIMSI
                     PEAMWKGLIPQEGVDSSHWVSARIGILTIAVIILWQFLVPKKIQFIPAPLVGVLVASG
                     EAYMLNLSIDYIEFRDSLGSSIHFLKMGDFASLLDPSILSLAIAIAFIASAETLLCAT
                     AVDQMHSGPRTRYNREMSAQGIGNLVCGFLGALPMTGVIVRSKANLQSGARTRASAVF
                     HGVWLLLFVGLFPEILELVPIASLAALLVYTGYTLINVQAIRTLSKFGRGEVVVYAVT
                     VVTIVTTNLLIGVLAGLGVALAKLIYSTNTLTVSVDRGTKNQPISIKFSGTATFVSLP
                     KFANALDNVPLKKEVHLDFDDLRYIDHACLNLLSSWKKFHETQDGKVSVDWVKVESKV
                     LERQTTPP"
BASE COUNT         1281 a         1138 c         1457 g         1328 t
ORIGIN      
        1 acaatcgatt ggtgtgcagt ttcctcaacc gaactaattt gtaatgactg gacgggggac
       61 ctcaaaaatc ccccgttcct tccggatgtg actgcgataa caaaaaagga gtctggcgcc
      121 aatctctagc gaaatcatga gggcaataat ggatcaaacg atctatgctt tgtgaggtat
      181 gggaaatgaa tacacgacag tttggcatat tagtcatgac gacgcttctg gccggggtgg
      241 tgggtggggc gttgtccaac cttgttttca tcaatgccgc agtatcggca gaggaatcag
      301 agcagccaaa gaaagttgtt acggcggagg tattccgcct tgtgacgaag gatggccaac
      361 cccgggccac gctgcttcta tggaatgggg aattgccggc tttgaccttc gctgatgaaa
      421 cctgtcataa ccgtgtgttc ctcggtgtct ttaatgaggc acagccggta ttgattctta
      481 acgataaagg ctgtaagcaa cgagcggctc tggatctgca gcctgatgga ttgccttccc
      541 tcactctcag agacaagaat gacgtgccac gtgcacggct ccgtgtgttg ctggatggaa
      601 gtcctgtcct gaccctcttt aatcagaacg gccagattgc ctggtcaccg cccttatcct
      661 cctccgaacc ttggagatcg actagagaaa ggtatcctct tggtagggac caggacatcc
      721 ccgaggaaca aacggacgtt tcaaacactt gactcaggcc atctgaaacg aacgcgttgt
      781 gtgaagttct tcttgaaaag aagtgaaaat catccaattt caatacttct tctgtaggaa
      841 tcaaaggaga aaagaccaat gagcaaagaa aagtttttac attttaattt gaggaggtca
      901 tatggctatt caaggtgtcc cagcctcagg gtttaagact gtcggacaga ttgtgccaac
      961 caatgaattg aaattcggta gaaaattgaa tgccctggct gcggcggtcg agttgctttc
     1021 tcaacatacc ccgggagggc cggtcgtcga tgaaaaaaac cattttatcg gctttatcag
     1081 cgaatttgat gttctccgtg ctttggaagc gggaaaagac ttgaacaaag tgacggtcga
     1141 agacgtgatg aaagaagatc atattgcaat tgctgatacg acgtcgatta aggatgccgt
     1201 gcatctcatg gaaaaacatc gattgttgag tctgccggta gagcgaaacg gggaggtgat
     1261 gtataccgtg acgaggcatg atcttttgcg ggcctggatt ggattaggat taggcataga
     1321 atctgggaat gagtagatca atggattggc gatcactccc agtatgggaa aacccatgta
     1381 atctagaaga cggacatgtt tattgagggg gaaatctcag agatgcggga gcaggggagg
     1441 gttggatcat actgagagtg gaaagtgaga cttcctgtct ttgtgagggc atgagcggtg
     1501 gtgcatgaat tcagaatcta acagctatgt caccgtcagg gtttttgagg cttcagatcc
     1561 ctgtgaatgg gaacttccgg ggccgaatgg aacggccatt gaaggatggc atcttctgtc
     1621 ggtggggtgt ggccattcgg ggaggtactc gcttcaggtg acctttgatc aatttcattg
     1681 tgtcaatgcg gagggccaaa aggtcaaggt acatttgagt ggggtttccg ctcagaagtt
     1741 ggagaaagaa ccatcccttc cgagtgcccg tacccatcgg ctcccacaag attgatgaag
     1801 aggttgagcc gatggaagag acgatggaag agacgatgga agagggggag ggtcgagaaa
     1861 agtcacatag agtctaaaat acttcgcaag tagatatttt taggtatcaa gggtttttca
     1921 cagctgcact gggaggtgtc tttttatagg ctgagtaaaa ggagcattga gagggtaact
     1981 atgccggctt ttgtgagaaa gagaagatat tgaaattaaa aaaaaagcga gagaattgtt
     2041 ttattgactt cttctcaggt tgcaagaggt aagcaacgct ttaatcagga gggatattta
     2101 tgcgtgcact ggcatatgtg ttgataggag tgttgggtgg gatgggtgtg atgagcctta
     2161 gccaaaaggg aggaccaagt cccactacta tgttggcacc ggtgagttat gcgatgatgt
     2221 ctgcggagcc agcttctgaa atagcagagg agaaaccctg gacgggtgcc gatttattag
     2281 agatggcgca gctctatgat caaaaagccg atgagcttca aagtgaagcg gtgagattgg
     2341 aacagcgagc cataagcctt gcagaaaagc ctcatatgga tcctaagggc tttaccagga
     2401 cggggtggat gctgatcgcc tcgtcgcgat ggaagtcggc aaaagagcta cgagaaatgg
     2461 ccgccatgca tcgttcagaa ggacaacgtc tcctggcatt ggaaaaaaac ggtgaggttc
     2521 ccacggagga tttggataag gagggcaatg cctctcagaa gcatggcaca taagttgtgg
     2581 tgtttttcag gtttacttta ggttagtgcc ccctgagttc gagttttgtc cttgtccact
     2641 ctgctttcgc agagagtggt attggcactg accagccggc ggcccttggt cgtgcccaag
     2701 acaatctttt gatacctcca ttatttgttc agcaggacca tggccgcgga aatgggagcg
     2761 gcgggaaatt tccttgctct atctctagat gaccattcta aggacagatc aaatcgtgag
     2821 ggaaatgtac ccgaaactca agggcgagga acggcttgcc gcgaggataa aaaagaatgt
     2881 gttggttcag cttgagcatt tgaagactca tcctgccgtc ccaagcggtt gcgcacgggg
     2941 aatctcacgc ttcatggatg gatttattct atcgcgacag gagaggcgtg gccgtgcgat
     3001 ccgcatcaag aaaagttcgt gtttccccaa gacgtcaaaa aggtcaagga tgcctagatg
     3061 cggacactct tcctcatgag gcataaggga tgactcatga ttaaaaaacc ggattggaaa
     3121 gagctgttca cgatgacctg gaaagatgtg caacgcgatt ttctggcgtc cgttgtggtg
     3181 tttctggtgg cattgccatt gtgcatgggg attgccattg cttcgggcgc ccctcccgtg
     3241 aaggggatta tcaccgggat tgtcgggggc ctcgttgtcg gactccttgc cggttgtccg
     3301 ctgcaggtga gcggcccggc tgccggtctg accgtgattg tcttcgatgt gattcagcga
     3361 tttggcctag gcaaactcgg gatcgtggtt ctggtggccg gaggaattca agtcctggcg
     3421 ggatttctcc agttaggtca atggttccga gccgtctccc ctgcagtgat tcaagggatg
     3481 ttagcgggaa tcggcgtgct catcctggca agccaatttc acgttatgat tggggacacg
     3541 ccaaaaggaa gcgggcttga gaatattatg agcattcccg aagccatgtg gaaaggatta
     3601 atccctcagg agggagttga ctcgagtcat tgggtgtcgg cccgcattgg aattctcacg
     3661 atcgcagtca ttattctttg gcaattcctg gtgcctaaaa agatccagtt tattcctgct
     3721 ccgctagtgg gtgttttggt cgcgagtgga gaagcgtaca tgctaaatct ctccatcgat
     3781 tatattgaat tccgggacag cctcgggagc tcgatccatt ttctgaagat gggggatttt
     3841 gcctcccttc tcgatccgtc tatcttgtcc ctggctatcg ccattgcctt tatcgccagt
     3901 gcggaaaccc ttttatgcgc tacggccgtc gaccagatgc attccggacc gcgaacccga
     3961 tacaaccggg aaatgtctgc ccagggcatt ggaaatcttg tgtgtggatt ccttggggct
     4021 ttacccatga ccggggtgat tgtccgcagt aaagccaatc ttcagagcgg ggcccgaacc
     4081 cgtgcctcag ccgtgttcca cggtgtctgg ttactcttgt ttgtcggact ctttcctgaa
     4141 atcctcgagt tggttcccat cgccagtttg gccgctctcc tcgtgtacac cggctatacc
     4201 ctcatcaatg tccaggcgat tcggacactt tcaaaatttg gccggggaga agtcgtcgtg
     4261 tatgcggtga ctgtggtgac cattgtgaca accaacctct taattggagt gcttgcaggg
     4321 cttggcgtgg cactggcaaa gttaatttat tcaaccaata cccttacggt ttcggttgac
     4381 cgggggacga aaaaccaacc gatttccatc aaattttcag gtacggccac gtttgtgagc
     4441 ctccccaaat ttgccaatgc tctggataac gttccgttga aaaaagaggt gcatctcgac
     4501 tttgatgatc tccggtatat tgatcacgcc tgtctgaact tactgagttc ctggaagaaa
     4561 tttcatgaaa ctcaagatgg aaaggtatcg gtcgattggg taaaggtgga gtctaaagtc
     4621 ctggagcggc agacgactcc tccctaattt aacagacatt tgacactcaa ttccctaaaa
     4681 aatggtccag gcatacgtgg aaggtcttgc cttttaaaga atatctggaa ttttcagcga
     4741 gacaaaccgt atggagagat gatctcggcg ggtttcccct tatggggctt tgctatgctt
     4801 tggggctgcc tacggtacct accgcatgga tgggaccatt tgaaagttta cggaatctcc
     4861 attttatgta tgtgaaggat aacatgaagg ggaatccagg ctacgcgatc gaagcatccc
     4921 tctgagtcat ttaatgatgg gagcctgagt gggctatgac caggggcccc tgaatgcctg
     4981 tttatcggat gtatgatttg tcgcgcatcc tggatcctga gggctattta cacattcttg
     5041 ttggatttca gggccaggac gtgagaatca ctcagtaatt ccaccgcgcg ctcggcggca
     5101 tcggcaaacg ggatgagcac gacatccacc ccggcctgtt tcaataattc cgcctcacgg
     5161 accgtatagg ctgccatacc taactgtttg gaataaccat gcgt
//
LOCUS       DATIAL010000335         2820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_491899, whole genome shotgun sequence.
ACCESSION   DATIAL010000335 DATIAL010000000
VERSION     DATIAL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491899"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VLA60_10450"
     CDS             <1..857
                     /locus_tag="VLA60_10450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF09827.1"
                     /translation="TTAPRMANVVSKVSQDLAVGMQFVFREKPVLRALLITTVFYTLG
                     ASAFIYVLPLFVNDHIEADPRWLGWLWSAMGAGMLTTSFGLSCVKNLGLNNRIFFIAV
                     SMVAGGGALQFLAHSHSVVWGFILVTLIGASTAAFTPIAWSIIQETTPNHLMGRVFTL
                     INTASMAMASGGMVAFGGVADNFGSMAGISMIGFVFLITGMVVVLLNRSSFQGERLLS
                     WKKMELSPYDDPKRVWSPVRISCSIHRSRRKNHQWRLDISPYSSDKIPNISPKRGSRA
                     PPWSKKYT"
     gene            893..1561
                     /locus_tag="VLA60_10455"
     CDS             893..1561
                     /locus_tag="VLA60_10455"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF09828.1"
                     /translation="MKHHSRDLLVMSLAVTLISWSQVFGSPTLLELTSSVPDETLVLQ
                     VQDHQAKPTPKLESSEKDPQKPSDKDNIVQSQNLSLKLAFMEDPRLFPYDIDCQVQEK
                     TVELRGEVSVEEEKTIVTAITTKLLKGKEIINHIEVRPSLRAAIQAKMDARLTELVKQ
                     RFAHSQTLRDADFEIMTIRGVVSLTGRTRFQVIVLEGAQAAREIPGVIAVNTQNVRLE
                     AGND"
     gene            1582..>2820
                     /locus_tag="VLA60_10460"
     CDS             1582..>2820
                     /locus_tag="VLA60_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF09829.1"
                     /translation="MSNLSLSSSDIPQPLALATNPPLLFQRNFGLLWWSQFISQIGDG
                     VTKLALLWFVYAVTGSPLQTTVIGILQTLPPILLGPFIGVFIDRFPKKIILISTDLIR
                     ALLIGLIPCLVSHEAFTVEYLYVLVFCNAVATSLFAPALFATIPFIVKKSALTAANAL
                     LQSTMSLGVIFGPLLSGLGIALLNSQDVLCLNVLTYIGSAICLVCISIPEVPHEAPFP
                     EATLKTSISSYVKDLQEGVQFTFFRQPVIRLLILTAGLYSFVISAFHTLLPVLGRNML
                     KLGPVEVGYITSGVGVGLFLASVFLAKVALGAPWQRIRLLVTTSLFTAFALWGFTGVS
                     ELSLSLLVVVLIGMGSGVLTPIEWGIIQEISPPQLIGRVLALYGTLAMLAAIGGMTTF
                     GWVTEQFGEFWTLLGMGMVLF"
BASE COUNT          648 a          702 c          681 g          789 t
ORIGIN      
        1 tgacgaccgc tcccaggatg gccaatgtag tgtcgaaagt tagtcaggac ttggcggtcg
       61 gaatgcaatt tgtctttaga gaaaaacccg ttcttcgcgc gctcctcatt acgacagtct
      121 tttatacgtt gggagccagc gcttttatct atgtgcttcc acttttcgtc aacgatcaca
      181 ttgaagccga tcctcgatgg ttggggtggc tgtggtctgc tatgggggca ggaatgctga
      241 ctacctcctt tggcctgagt tgcgtgaaga acctgggact aaataatcga atctttttca
      301 tcgccgtttc aatggtcgcg ggaggaggtg cgttgcaatt tctcgctcat tcacactcgg
      361 tcgtatgggg ttttatcctc gtaacactta ttggggccag tacggctgcc ttcacgccca
      421 ttgcctggtc tatcatccaa gaaacaaccc ccaaccattt aatgggaaga gtgtttacct
      481 taatcaatac tgcgagcatg gccatggcca gcggaggcat ggtggccttt ggaggggtgg
      541 ctgataactt tggttcaatg gctggcattt cgatgattgg tttcgtattt ttgattacag
      601 gcatggtcgt ggttttattg aatcgctcct cctttcaggg agaaaggctt ctctcatgga
      661 agaaaatgga actttctccc tatgatgatc cgaaaagagt atggagtcct gtcagaattt
      721 cctgttccat ccatagatca aggaggaaga accaccagtg gaggttggat atctcgccat
      781 attcttctga taaaataccg aatatttccc caaaacgtgg gtcgcgcgca ccaccatggt
      841 caaaaaaata cacataataa attcatgggc tattcagaag gagaagttta gaatgaaaca
      901 tcactctcgt gaccttttag tcatgagtct ggctgtgacg ctaatttcct ggagccaggt
      961 attcggctca ccaaccctct tggaattgac ttcctctgtt cctgacgaga ctcttgtgct
     1021 ccaagtccaa gatcatcaag cgaaacccac cccgaaactg gagtcttccg agaaggaccc
     1081 gcagaaacca tcagacaaag acaacattgt ccaatcccag aacttgtcat taaaactggc
     1141 atttatggag gatcctcgcc tctttccata cgatattgat tgccaggtcc aggaaaaaac
     1201 ggtggagttg agaggtgaag tttctgtgga ggaagaaaag accatagtca cagccatcac
     1261 caccaagctg ctcaagggga aagaaattat caatcacatc gaagttcgcc cctctttgcg
     1321 cgccgccata caggccaaaa tggacgctcg cctgactgaa ttggtgaaac aacggtttgc
     1381 tcatagtcaa acactgcggg atgccgattt tgaaatcatg acgattcggg gagtggtatc
     1441 tctgaccggg cggacccgat ttcaggtgat tgtcttggag ggggcacaag cggcccgtga
     1501 aattcctggc gtcatcgcag tcaatactca aaatgttcgt ctcgaggcag ggaatgattg
     1561 aagggaccca atcgccacat catgtccaat ctttctctat cttcttccga cattcctcaa
     1621 cctctagctc tcgccactaa tccccccctt ttatttcaaa ggaattttgg acttctttgg
     1681 tggagtcagt tcatttctca aatcggggat ggggtgacca agctggcctt gttatggttt
     1741 gtctatgcgg tgaccggttc ccctctccaa accacagtca ttggaatctt gcaaacgctc
     1801 cctccgattt tactcggtcc cttcataggg gtatttattg atcgttttcc caaaaaaatc
     1861 attctcatca gcaccgacct catacgagcc ctgcttattg gcctcattcc ctgcctggtc
     1921 tctcatgagg cgttcacggt tgaatatttg tatgtcttgg tgttttgcaa tgccgtcgcg
     1981 acttccttat tcgcgccggc cctattcgcc accattccat tcatcgtgaa aaaatcggct
     2041 ttaacggctg ccaatgcttt actgcagagc accatgagtt tgggagtgat tttcggcccc
     2101 ctgttaagcg gactgggcat cgccctgtta aattcccagg acgttctctg cctcaatgtc
     2161 ctgacgtata tcgggtcggc catatgcctt gtatgtattt ccattccgga agtgccacac
     2221 gaagcgccat tcccggaggc aaccctaaaa acttccatca gcagctatgt caaagaccta
     2281 caagaaggcg ttcaatttac tttcttccgt cagcctgtta tccgtctgct tattttaaca
     2341 gctggattgt atagcttcgt catcagtgcc tttcatacat tattgccggt cctcggcagg
     2401 aatatgttga aactgggtcc ggtggaggtc ggttatatca cttccggggt gggtgtgggc
     2461 ctatttttgg cctccgtatt ccttgctaaa gtcgccctgg gagcaccttg gcaacgcatt
     2521 cgcctgctgg tgaccactag cctattcacg gcattcgctc tttggggatt caccggggta
     2581 tcggagttat ccctcagcct cctggtcgtc gtccttattg gaatgggaag tggggtcttg
     2641 accccgattg aatggggaat aatccaggag atttctcctc cacaacttat cggacgagtc
     2701 ctggccctgt atggaacatt ggccatgctc gctgccattg ggggaatgac cacatttggt
     2761 tgggtgacgg aacagttcgg agaattttgg actctactgg ggatgggaat ggttctattt
//
LOCUS       DATIAL010000336         2183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1029294, whole genome shotgun sequence.
ACCESSION   DATIAL010000336 DATIAL010000000
VERSION     DATIAL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029294"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VLA60_10465"
     CDS             <1..395
                     /locus_tag="VLA60_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09830.1"
                     /translation="LTHCGYEVMIAKDGEEAVALYQQAMETHRPFLLVILDLTVPGKL
                     GGMGTLTRLQQLDPQVRALVSSGYSNDPIMANYASHGFVGVITKPYSLIELSNMVHQR
                     LWRQAPLATGQTPSDPPGRMTSISPSPH"
     gene            605..1972
                     /locus_tag="VLA60_10470"
     CDS             605..1972
                     /locus_tag="VLA60_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HSF09831.1"
                     /translation="MSLSHLKKTRVIVQQANPAAKRILKSLIDAGLEACDPALAIATS
                     LHLTKNRLQVHDLHYNLTHYSRIVCVGAGKASARMARALEQMLGNRLEGGMVIVPDGH
                     DAPCDKICMFNAAHPTPDGRGVKATKQLLALIQSLSQHDLLIVLLSGGASSLLCAPSI
                     GLTLAAKRRTTQLLLRSGATIHEINVVRKHMSAIKGGQLAQATPATILTLIMSDVLGD
                     DLSTIGSGPTVTDPSTFQDAIDILQQYSIWRKVPESVQRHLQRARSKPIPAPLKATRQ
                     QALRNRHIVLANNRHALEGVAKIAKLLGLKPFILPYPLQGEAQEIGSILGGLARDLVE
                     FGNPVHPPACLIAGGEPTVAVNGKGRGGRAQECVLAAARELAGLTNVFVAGFGTDGTD
                     GPTDAAGAVVDGKTVQRAKKKGLSIEHALHEHDSYTFFQQIRGHIMTGPTGTNVNDIY
                     LIVAM"
BASE COUNT          557 a          631 c          511 g          484 t
ORIGIN      
        1 tgttgacgca ttgtggatac gaagtgatga tcgcaaagga tggagaggaa gcggtggccc
       61 tctatcaaca agccatggag actcatcggc cttttctctt agtgatctta gacttaacgg
      121 tgccgggaaa acttggcggt atgggaacct tgacacggtt gcaacaactt gacccacagg
      181 tcagggcact cgtctccagt ggatattcaa atgatcccat catggccaat tatgcctccc
      241 atggattcgt cggggtgatt accaagccct attcgctgat cgaactgagc aacatggttc
      301 accaaagact ctggcgtcag gctcccctcg caaccggaca aaccccttcc gacccaccag
      361 gacggatgac ctctatctcc ccttcccctc attaatttgt gttccgggct attccttcat
      421 acacccggtg aacgaaccga cgacttctca cctctttgga aaaacccctt gttttgacta
      481 agggtggcag aagttcacct tgcatttctc aaactcattt ctaatgataa tatgctacat
      541 tgcagacaat gtttccccaa cagccatcac gctcttcacc ataaattctt gatcaaccac
      601 ctccatgtca ttatcacacc tcaaaaaaac acgcgtcatc gtccagcagg ccaatccggc
      661 cgcgaaacga atattgaaat cccttattga tgcgggcttg gaggcgtgcg atcctgctct
      721 cgccatcgca acttccctcc atctcaccaa gaaccgactt caggttcatg atctccacta
      781 caatctgact cactatagtc gcatcgtctg cgtcggagcg ggaaaagcct ctgcacggat
      841 ggcgagagca ctggaacaga tgctcggaaa tcggcttgaa ggcggaatgg tcattgtccc
      901 ggacggacat gatgccccat gcgacaagat ttgcatgttt aatgccgcac atccgacgcc
      961 ggacggacga ggggtaaagg ccacgaaaca actcctggca ctgattcaat cgctttctca
     1021 acatgatttg ttgattgttc tactttcagg tggagcttca agcctgctct gcgcgccatc
     1081 aatcggcctc accttagctg ccaaacgacg gaccacccag ttactcctgc gatccggagc
     1141 caccattcat gagataaatg ttgtccgaaa acatatgtcc gccatcaaag gcgggcaatt
     1201 agcccaagcc accccggcaa cgatcctgac cttaatcatg tccgatgttt tgggagatga
     1261 cctatccacc attggatccg gtccgacagt cacagatccc tccacgtttc aggatgccat
     1321 agacattctt caacagtatt cgatttggcg caaagtgcct gaatccgtac aacgccactt
     1381 acaacgagca cgttccaagc caattcccgc accattgaag gcaaccagac aacaggcctt
     1441 acgaaatcgg catattgttc ttgccaacaa ccgtcatgcc ctcgaaggcg tggccaaaat
     1501 tgccaagctg ttaggactca aacccttcat tctcccctac cccttgcaag gggaggcaca
     1561 ggagatcggc agcatcctgg gaggtttagc cagggacctc gtcgaattcg gcaacccggt
     1621 tcatccacca gcctgtctca tcgccggagg agaaccgacg gtcgccgtga acggaaaagg
     1681 ccggggtggg cgggcacagg aatgcgtcct ggccgcagcc cgggaattgg ctggactcac
     1741 gaatgtattt gtggcagggt ttggcactga cgggaccgat ggaccaaccg atgcggccgg
     1801 tgcggtcgtc gatgggaaga cggttcagcg ggccaagaag aaagggctct ccattgagca
     1861 cgcgttacat gaacatgaca gctatacctt cttccaacag atccgcggcc acatcatgac
     1921 cggccccacc ggcacgaacg tgaatgacat ttatctgatc gtcgccatgt aaaagattat
     1981 ttttcatttc tgcgccttta taagtaagtg ccagaggagg tcaagaacat tgaacgacgc
     2041 gcaacgcatg gtccaagaat tccacaagca attcgaaatc cacgtgtctc ctactccttc
     2101 tatcccggat gaaccgaccc aaatccttcg gaaacggctc atccaggaag aattcgatga
     2161 acttcaggaa gccatgcagg aaa
//
LOCUS       DATIAL010000337        12121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1029296, whole genome shotgun sequence.
ACCESSION   DATIAL010000337 DATIAL010000000
VERSION     DATIAL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029296"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VLA60_10475"
     CDS             complement(<1..1193)
                     /locus_tag="VLA60_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF09832.1"
                     /translation="MTMRREWMRILIVQDRPDQLEVLVNVLGEEGYQVVGARTAAEAS
                     EYAKCESFAVAVVDVQAPDASEVSLLSLLKAKNAEIQMILSPAFASLDSAKDVIGESA
                     VAYLKREGSTSELLSTLRRTFRNQLTEYTKELEIALAERDERFRQLVDHIKEVFWVFA
                     PDQSEVWYISPQYETVWGRSCASLLEDPRSLWNAIHHHDQPRFHTHQLAPDVNGVQGE
                     CRIVRPDNSVRWVRIQTVPIRKANGDLLSLAGVAEDITERKRVEEALTKTERQFRQSS
                     RMEAIGTLAGGIAHDFNNILTAILGYTELALATVPKESRTQRNLQEVLTAGHRAKHLV
                     LQILTFSRQAGQGKKPTPFHMVVREALKLLRSTIPTTIEIRQVLNTEATILADPTQMH
                     QIIINL"
     gene            complement(1341..1616)
                     /locus_tag="VLA60_10480"
     CDS             complement(1341..1616)
                     /locus_tag="VLA60_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09833.1"
                     /translation="MHRTVMHRFSEYHKGAIGIASGPLPNIHFPGSPYPFLLMSRGVT
                     INAGIVMMGPDDWEMLGIDFRMAMSRDQPRVRKQHSVSLMLKDDAPE"
     gene            complement(1823..2593)
                     /gene="pyrF"
                     /locus_tag="VLA60_10485"
     CDS             complement(1823..2593)
                     /gene="pyrF"
                     /locus_tag="VLA60_10485"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HSF09834.1"
                     /translation="MVIPIQERLIVALDVPSIEEARQYVKDLEGVARFYKIGLELFLA
                     VNADFIKELRAQNCRIFLDLKMNDIDETVRRAVNLAANLEVDFLTIFGNHATAKAAVL
                     GRGDGAIQILTVPLLSSWGEKDLQDLGMLSVEPGVPARFHSLDDYVLWRADMAMAQGC
                     DGLIASGKYVGILRERFGRKALIVSPGVRPAGQNTHEHQRSLTPFEAIRAGADYLVVG
                     RPIRDADNRRKMAEGIQEEIALAVKAGPSKERTLAGER"
     gene            3407..4258
                     /locus_tag="VLA60_10490"
     CDS             3407..4258
                     /locus_tag="VLA60_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09835.1"
                     /translation="MANASPIPFSSSSSSSSPILVVDDEQDIVLTLRDLLEADGHHIN
                     VASTGGTALECVKNHSPSVVILDVKLPDMDGLLVLEKMTKAVPGLPIIILSSYTTLDD
                     VTGPLEEQGAFAYLHKPINRSEIRTTVRQALKAYDLAKKMERARHALYESEIRFQAVF
                     QAAIDAIVLADHTGRIMSWNKAAETMFEYTAEEMFGKPLTLIMPSRYREAHTNGMKRL
                     QATGESHVIGKTVTLHGLRKSGQEFPIELSLNSWMTGTHPSYSGFIRDLSFRESKKER
                     PLQYHNV"
     gene            complement(6068..6141)
                     /locus_tag="VLA60_10495"
     tRNA            complement(6068..6141)
                     /locus_tag="VLA60_10495"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6107..6109),aa:Gln,seq:ttg)
     gene            complement(6417..7079)
                     /locus_tag="VLA60_10500"
     CDS             complement(6417..7079)
                     /locus_tag="VLA60_10500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF09836.1"
                     /translation="MMERVLEPEVMDDPVQVQAYAEADFQEENQGFVDTFLRLYEDQD
                     GPHVVDLGCGPGDIAIRLARNHPTCRITGIDASIPMITWAEQAVKQAGLAHRIELLSQ
                     RFQDVRLATPADAVMSNSLAHHVPNPLRFWYEIKKMIKPGGLVVVMDLLRPDSPEEAQ
                     ALVEQYAAHEPERLRQDFFHSLLAAFTEDEVAAHLAELNLSRLMVDVLDDRHWIVYGR
                     VY"
     gene            complement(7076..7621)
                     /locus_tag="VLA60_10505"
     CDS             complement(7076..7621)
                     /locus_tag="VLA60_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HSF09837.1"
                     /translation="MATILFVSDKYAGDVKIFMGSVAYLYQINCSAGGLPKLPVPEAW
                     ITFKGMDGDRHRNRALHGGPDRALRLFSFEVLEALQHEGHTVHPGASGENLTVAGLNW
                     TELKPGDLLKIGEEVQIKLTSYCEPCRHNAQWFVNGDFSRISHRQHPGWSRLYARVLS
                     EGRVRQGDRVWIVTLPKRNMK"
     gene            complement(7668..8429)
                     /locus_tag="VLA60_10510"
     CDS             complement(7668..8429)
                     /locus_tag="VLA60_10510"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF09838.1"
                     /translation="MFEGVFVFQPSAWEDRNWASLSGLPLEEVWLPVDESVTIFGWFV
                     NAGPTKPVLLWCHGNAGNISHRLDNIRELYRRGLSVFIFDYRGYGRSTGTPSEPGMYQ
                     DALAAYDYVLNRRGIASSRIVVFGRSLGACVAGEVAILRTSAGVILEGAFPSIQAMSD
                     HHYLGLPARWLLNVDFNLTEKLAKLRRPLLVIHGEKDSIVPVALGRQVYDAAHEPKQW
                     FVVVGAEHNDVPFVGGTAYFQKITSFVQTLISKEN"
     gene            8532..9338
                     /locus_tag="VLA60_10515"
     CDS             8532..9338
                     /locus_tag="VLA60_10515"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HSF09839.1"
                     /translation="MATYAIGDVQGCVQALHRLIEQIRFNPASDRLWFVGDLVNRGPD
                     SLEVLRLIKQLGNSAITVLGNHDLHLLAVWAKVTTLSRKDTFQSVLSAPDVNDLLTWL
                     RFRPLVHVEDGFCMVHAGLLPSWSISQVLELAREVEEVLQDENFHQHLPAIYFRKAIV
                     FSPHLSLDERLGLTTNVLTRLRVCTQEGIPEFSFKGHPDDAPPGYRPWFQVPNRATEE
                     DTIIFGHWSALGVVKGPRVFAIDGGCVWGRELIALRLEDQQLFRVTCSKI"
     gene            9717..10058
                     /locus_tag="VLA60_10520"
     CDS             9717..10058
                     /locus_tag="VLA60_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09840.1"
                     /translation="MMKSILQQRTNSSANNSATTHRKIRRRHTRIHLDSLRPDPSAYC
                     ADALAQCPKCQGLVHQEGGETSNETVVRCLNCGWQPHYQTPIIRETAESRLIRSLTAQ
                     FVSEYEWHRVP"
     gene            10628..11530
                     /locus_tag="VLA60_10525"
     CDS             10628..11530
                     /locus_tag="VLA60_10525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HSF09841.1"
                     /translation="MIVCVGLFLDLSTVTSAEEVAILKSADISAYSEAITAFKSALPP
                     SLHVTSEYDLKDDMAEGRNLARRIRASNAKVVLAVGLKAAVTAKLQIQKIPVIFCVVI
                     NPKKYGLPTDNMVGLSFRIPFHQRLQTLQTLVPNVSRIGVLFDPEKTKGVLNQLLQDA
                     EASGIRIVTEEVSAEKDVSKALNAIKNRIDALWLLPDSTILNENTLDFLLSTTLEANV
                     PLLGFSADLVRSGAVVGTYFRYADIGKLAAQLSHQLARQTSPSQLGTIISPGRVRRSI
                     NLRSARHLGLSITFDLLRQFDEQF"
     gene            11517..>12121
                     /locus_tag="VLA60_10530"
     CDS             11517..>12121
                     /locus_tag="VLA60_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09842.1"
                     /translation="MSNFNPPHAHPSPPLPTEPAGLEPGYLTLRTKFSLFVSLVIVLA
                     CSSLSGYLIQQEAEVMETSLLNTGKVLVKTLQNISLNRLINQDLHYLERMLEGAISLS
                     EVVYAIARDQDGKVLVEKSKGRLVDITKLRRDETQPLFPDDSLTETLITAASKISKIE
                     PLVTVLYSSPHQKGLLPKRSAEKKSAEGFMFGSETIYDFALP"
BASE COUNT         3176 a         3242 c         2698 g         3005 t
ORIGIN      
        1 agattgataa tgatctgatg catttgggtc ggatccgcta gaatggtggc ctccgtgttc
       61 aatacttgac ggatctcaat ggtcgtggga atcgtggatc gtaagagttt tagagcttcc
      121 cggacgacca tgtggaaagg tgtgggtttc ttcccctgac ccgcttgacg actaaaggtc
      181 aaaatttgaa gcaccaggtg tttggctcgg tgacccgcgg tcagcacttc ttgcagattt
      241 cgttgagtac ggctttcctt tggcacagtg gccaaggcca attcggtata acccaaaatt
      301 gcggttaaaa tattattgaa atcatgggcg atgccgcctg ccaacgtccc aatggcttcc
      361 atgcggcttg attgtcgaaa ctggcgctcg gttttcgtca gagcctcttc cacacgcttc
      421 cgttctgtaa tatcctccgc cacccccgca agactcagca aatccccatt agcctttcga
      481 atgggaaccg tttgtattcg cacccaccgc acggagttat ccggtctcac aatccggcat
      541 tctccctgaa ccccattcac atccggtgcc aattggtgtg tgtgaaatcg tggttgatcg
      601 tgatgatgaa tcgcattcca taggcttcga ggatcttcca aaagactggc acagctccgg
      661 ccccagaccg tttcatattg agggctgata taccagacct cagactggtc aggggcaaag
      721 acccaaaaaa cttcttttat atggtccaca agttgccgaa accgttcatc ccgctctgcc
      781 agcgcgatct ccagttcctt tgtgtattcc gtcagttggt tgcggaacgt tcgacggaga
      841 gtactcagca attctgatgt ggacccttcc cgtttaaggt aagcaacggc gctctcacca
      901 atgacatctt tagctgaatc cagactagca aaggccggac tcaggatcat ctgaatctca
      961 gcattcttcg ccttgagaag agaaagcaat gagacttcgg aagcatccgg cgcctgcaca
     1021 tcgaccactg cgaccgcaaa gctttcacat tttgcatatt ccgaagcttc cgcggctgtc
     1081 ctggccccta ccacctgata gccttcctcc cctagaacat tgacgagcac ttccaattgg
     1141 tcaggacggt cttgcacaat gaggattcgc atccattccc ttcgcatcgt catatatccc
     1201 tccattatgt ctaccgtcct gaactgtgtg cgggaccctc agaatttatt caaagatgta
     1261 ttggaagctt tctgccatac aggtgtttaa acctagactt cccaagccat gatccacttg
     1321 gatttccacc tctgctccgg ttactccggc gcatcgtcct tcagcatcaa cgagacagaa
     1381 tgttgttttc gcacacgggg ttgatcgcgg ctcatggcca tccggaaatc aatccccagc
     1441 atttcccaat catccggccc catcataaca ataccggcat taatggtcac gcccctgctc
     1501 atcaacaaga agggataggg gctaccggga aaatgaatat tggggagggg tccgctggct
     1561 attccaatcg ctcccttgtg atattcggaa aaacggtgca tcacggtccg gtgcaccaag
     1621 acgtcaccat acggtcgaaa ttacttaata gaagaggata agtatcaaca gttggccatt
     1681 cagggagtga ggcaaccaac atagagacaa aagggagcgt gagaaaggct agaccttccc
     1741 ctaaaataag gaagggaaaa aacgtgcagg aaatttaatg aagggccact ttggttcttt
     1801 aagactcatg ggatgttgcc acctatcttt ctccagccag tgttcgctcc ttcgaaggac
     1861 cagccttgac cgctaaggca atttcctctt gaatcccttc ggccattttg cgtcgattgt
     1921 ccgcatcgcg aataggtcgc ccaacgacta aataatcggc cccggctcga atggcttcaa
     1981 atggagttaa cgaacgttga tgttcatggg tgttctgtcc ggcaggtctc acgcctggag
     2041 agacaatgag cgccttccga ccaaaccgtt ctctcagaat tccaacatat ttccctgagg
     2101 cgatcagacc atcgcaccct tgggccatcg ccatatcggc tcgccataag acgtagtcat
     2161 ccagagaatg aaaacgtgcg ggcactcctg gttccacact caacatccca aggtcttgaa
     2221 gatccttttc cccccaactt gaaaggagcg gaacggtcaa aatttgtatt gccccgtcgc
     2281 ctcgacccag cactgcagct ttggctgtgg catgatttcc aaatatggta agaaaatcaa
     2341 cttcaagatt agccgccaga ttcacggctc ggcgcacggt ttcatcgata tcattcatct
     2401 tcagatctaa aaagatccgg caattttgtg cacgtaattc cttgataaaa tccgcattaa
     2461 ccgccagaaa taattccagg ccgattttat aaaaccgggc gacaccctct aaatccttca
     2521 catattgccg ggcctcctct atggagggaa catcaagtgc gacaattaac cgttcctgta
     2581 tggggataac catgttcatt cccaattcac catatacgct agcgggtaaa cattcatggg
     2641 catgagcgga tgaggaaact atatacaacc taaggaaaga gagccgcggg cattatccca
     2701 gactttcata ccagtaaata gccacacacc caatttccgc atgaacatgc cataattcta
     2761 gttatcaaca gtggagagtg ccataaccaa tggatgaact caagccacac cacatcgagg
     2821 ccaaacctgc ccttgacgac tcattgatcc gaaccaattc gccgaaaatt cctggacctg
     2881 cataggtttc cgggatcgac ttcaatacac accctacccc acaggatacg tatcccacat
     2941 ttttacggtt ctgtcactca tggcaaaagc gccgattccg gttacgaagc caaatgagat
     3001 cagtgctctt cccaattttt cgtcgggtgg aatccgattg ttgaggcttg gagacagata
     3061 gttgatctat taaaggaaac agatcgaaaa gtcagggaaa attcgccagc accgttgtcg
     3121 gtgaattcca tctcgcggag tatacaggtc aagggtgtgt atccatttgg ataattgggt
     3181 gtatccaaac agatataaaa atttcatttt ttcataagca agtcaattcc acaggggctt
     3241 ttccttgtaa aattttcaca aaatcacaat tttctccaaa ggcttatctc ctgaccgatt
     3301 ccacctatgc tgtaattggt acgtagattg cgtgttcaat cattataacc ccattctcag
     3361 gaacatccgc gttgaggggc agttacccca gacatgaggt caaaccatgg caaacgcatc
     3421 cccgatacca ttctcttcct cttcctcttc ctcttccccc attctcgtcg tcgacgatga
     3481 acaggatatt gtcctgactt tacgagatct tttggaagcc gacgggcacc acattaatgt
     3541 ggcttcaaca ggcggcaccg ccttggaatg cgtaaaaaat cactcgccta gtgtagtaat
     3601 ccttgacgtc aaactccctg acatggatgg cttactcgtt ctagagaaga tgacaaaagc
     3661 ggtaccaggg cttcccatca ttattttgtc gagttacacc actctcgatg atgtaaccgg
     3721 cccccttgaa gaacagggcg cctttgccta tctccataaa cccatcaacc gttccgaaat
     3781 tagaactacg gtgcgtcaag cactcaaggc ctatgacttg gcaaaaaaaa tggaacgcgc
     3841 ccgtcacgcc ttatatgaaa gcgaaatccg ctttcaagcc gtctttcagg cagccataga
     3901 tgccattgta ttagcggatc acaccggccg catcatgtcc tggaataagg ctgctgaaac
     3961 gatgtttgaa tacaccgccg aggaaatgtt cggaaaacca ctcacactga tcatgcccag
     4021 tcgctacagg gaagctcata ccaatggcat gaaacggtta caggcaactg gcgaatctca
     4081 cgtcatcggg aaaacggtca cactccatgg attaagaaaa agcggacagg aattccccat
     4141 cgaactctct ttgaattcct ggatgacagg gacgcatcct tcctattccg gatttattcg
     4201 agacctctca tttcgagaat caaaaaagga aaggcctttg cagtatcaca atgtatgaga
     4261 cccctggacc gggcatggct cgtaattttc tgagcggtga ggcaaagaga ttcgtctgag
     4321 caagcatgga ctgaagaaga gcgatcccaa gcaagttaaa ggattcggtg cgaggattag
     4381 ataattcctg ccgtgaaacc ctttcaattt gactcagact tgtgttcgtg ggaacacccc
     4441 gtcgagattt gaatttggtc gtacatcgcc tttaacattg tgactccttc ggttcacctg
     4501 tccaggcgga tgtaatccct ctatcgaaaa cacacgttat ttgtcgtagt ttttcctcac
     4561 actcaggtcc cctgagaact gcttgggcct gctcattccc atcgcaaaat ttccacacaa
     4621 tcgaaaaatt aatagtcaat cggatttcaa tgtagcaagg gctggctcaa gctttggaag
     4681 atcatcattc aaaaatctca gatcggttga aaatatgtcg tacttggcta actcaggcaa
     4741 aggaacgaac cgtatatttt tgatctcggg ggagcggggg cgaatgtgcc cggaattcag
     4801 tctcgcacgg aagattgcga aaccgtcctg tggagcacag cggaagtaaa agtgcaatcg
     4861 aaaggggccg ggttcttccg gaatataggg aatagcatca attctaagga gactgttcac
     4921 cctaatggcg ataccgagtt cctcggacat ttccttgcaa agagctgcct gtataacatc
     4981 gtcaggtgat gaagtgttgt ttggtctggt cggatctccg ggagttgatt cgagagaccc
     5041 accaggaaga ccccatgctc ctttcggccg ataactgtgc tcaactaaga gcacctccgg
     5101 aggggatgaa ttttcgtcga tcatgacggc tattgccccc actacccagc ttctggcaag
     5161 taacccttta aaaaatcctg ccacccgtgg aggaaacagc ctccaaatat atagggcaaa
     5221 acgatgaagg tttgggtgat gctcaataaa ccgtgttaac catgcgaaca tatcttttta
     5281 cgttatctat gaacagccaa gccagcacgg agatttggtg gatgtttcaa gaggtccgtt
     5341 aacttttttc caagtacctg tccttttgtc tcatcactat gcctggggtc aataacgcca
     5401 atacactata gttagctatc caagacaaat tgaatcatca attttcaatc aaaaatcacc
     5461 attcggtttt gccacttcga ttggattcac catagctatc ggcttatagg ccacacacat
     5521 tgctacgtga tagtttattg cccctgccgc tgatgggacc tttgtcccaa gaagaaatta
     5581 ggtcccatat ctgggcaaaa ttcccttatt cttttgattc gaattttata cgctcatatg
     5641 caacagaatg agaggattcc atactgtgta aggatctcct tgctgaggta aacctttcag
     5701 aacgggatcg ttaaaatttt cattgccgtg aagcggctac acattttttc tctaccgggt
     5761 cttgattcat cctgaaaatt gaatactcat cctggatgac ctagctgcgg ggttgtccac
     5821 cggtagatgt ttcggtcgtt ggtcctgaga atggtaaatc tgactatccg ttaaaaataa
     5881 cacttgagca tcggaatggc cccacaacca ttcgatatgc tcatctcagg ctcggcttct
     5941 aaagaggcaa tcaacgtatg taaaattgtc agaaacttgt cccaaatcct ggaccagtga
     6001 ttcaggcaag caattaaagg aaactgaaga agtaagtgaa gtgcctgaaa ggacagagga
     6061 aattgactgg ctgggggacc aggaatcgaa cctgggttct aagatccaaa atcttatgtc
     6121 ttgcccctag acgatccccc aaccgaacag gcactttaag acttacgggt ctaaagcgtc
     6181 aagatctttc aagcgagtgt tgaaaaagca tgccctgagt tttgccgaag ggtccgtcag
     6241 cggctttctc gcccgtttat catgctcacg tgcttaccta aacacttcgt gcctaaacgg
     6301 ctgcggcctt gctggacgta gatttttgaa cactccattg ctctcgattg ccacgaaaga
     6361 atatggtgaa gcttaaaact gttaaaaatc tgaattattc aacggatctt tcaaggttag
     6421 tacacacgcc cgtacacgat ccaatgccgg tcatccagaa catcaaccat caggcggctt
     6481 aaattcagtt ctgcgaggtg ggcagccacc tcatcctcag taaatgccgc caatagggag
     6541 tgaaagaaat cctgccgtaa tctctctggt tcatgagccg catactgttc aacaagcgcc
     6601 tgcgcttcct cgggagaatc cggacgaagg agatccatga ccaccactaa cccgccgggc
     6661 ttgatcattt tttttatctc ataccaaaat cgtaacggat tgggaacatg gtgggccaaa
     6721 ctgttcgaca tcacggcgtc agccggagta gccaatctga catcttgaaa ccgttgcgag
     6781 agaagctcta ttcgatgagc taacccggct tgcttcactg cctgttccgc ccaggtgatc
     6841 atgggaatgg aggcatcaat tcctgtaatt cgacaggtgg gatgattccg ggccaggcgg
     6901 atggcaatat caccgggacc gcaccccaaa tccacaacat gagggccatc ctggtcctca
     6961 tagagccgaa gaaaggtatc cacaaacccc tgattttctt cttgaaaatc cgcttcggca
     7021 taggcctgaa cttgcaccgg gtcatccata acttccggtt cgagaacccg ttccatcatt
     7081 tcatattcct tttcggcaac gtcacaatcc agaccctatc cccttgccgt acccgtcctt
     7141 cggacaagac ccgagcatac aatcggctcc atccggggtg ttgccggtgc gagatccggg
     7201 aaaagtctcc attcacaaac cattgcgcat tatgacgaca cggctcacaa taacttgtca
     7261 gtttgatctg cacctcttct ccaattttca gaaggtcacc tggtttcagc tctgtccagt
     7321 ttagtccggc cacggtcaaa ttttctcccg aggccccagg gtgaacggtg tgcccctcgt
     7381 gttgtaaggc ctccaggacc tcaaacgaga acagacgtaa ggcccgatcc gggccgccat
     7441 gcaaagcccg atttcgatgc cggtccccat ccatcccttt gaacgtaatc cacgcctcgg
     7501 gcacgggcag cttggggagt ccacctgcag aacaattaat ttgatagaga tacgcgacag
     7561 aacccataaa aatctttaca tcaccggcat acttgtccga gacgaagagg atggtagcca
     7621 tcgatcgaaa gggtggtcaa gcaggactac ataaatcacg aagaacctca attctctttg
     7681 gagattaaag tctgaacaaa gctggtgatt ttctgaaaat aggcggttcc cccaacaaac
     7741 ggcacatcat tatgttccgc acccactacc acaaaccatt gtttaggctc atgtgctgca
     7801 tcatagacct gccgccccag ggccaccgga acaatggaat ccttttcgcc atgaatcacc
     7861 aatagcggac gcctgagctt tgccagcttt tcagttagat tgaagtccac atttagcaac
     7921 caccgggcgg gcaatcctaa gtaatggtgg tccgacatgg cctggataga aggaaatgcc
     7981 ccctcaagaa taactccggc cgatgtccgg agaatggcca cttcaccggc cacacaagcg
     8041 cccaaagacc gtccaaacac gacaatccgg ctcgaggcaa taccacgtcg gtttaagaca
     8101 tagtcatagg cggccagggc atcctgatac atgcctggct ctgaaggagt gcccgtgctc
     8161 cggccatagc cacgataatc aaagatgaaa acagataatc cgcgccgata cagctcccgg
     8221 atattgtcta accgatgact aatattaccg gcattcccat gacaccagag aagcacaggc
     8281 ttggtcggtc cggcgttaac aaaccagccg aatatggtaa ctgactcatc aaccgggagc
     8341 cacacctctt ctaaaggcag tccactgaga ctcgcccaat tccggtcttc ccatgcagag
     8401 ggctgaaata caaaaactcc ttcaaacatc cgcccattgt agcaaggcgg ggacaccaac
     8461 gcggataaaa aatgctaaat tagggtattc ttcccgtaga aattcctttt accattcggg
     8521 gatccttcac gatggctact tatgcaattg gagatgtaca gggatgcgtc caggccttac
     8581 accgtctgat agaacagatt cgcttcaatc ccgcatccga tcgtctctgg ttcgtcgggg
     8641 acttagtcaa tcgaggaccg gattccctgg aagtgcttcg cctcatcaag cagttaggga
     8701 actcggccat caccgtgttg ggcaatcatg acttacatct tctggcggtg tgggccaagg
     8761 tcaccaccct tagtcgaaag gacaccttcc aatcggtgct ctcggcacct gatgtgaatg
     8821 acttactcac ctggttgcgg tttcgacccc ttgtccacgt cgaagacgga ttttgcatgg
     8881 ttcatgccgg cctcttacca tcctggtcca tttctcaagt cctagagtta gctcgtgagg
     8941 tggaggaagt cttgcaggat gagaactttc atcagcacct accggcaatc tattttcgaa
     9001 aagccatcgt attttctcct catctgagcc ttgacgaacg attaggcctc accaccaatg
     9061 tcttgactcg cctccgggtc tgtacccaag aaggtattcc tgagttctcg tttaaggggc
     9121 atcccgacga cgcccctccg gggtatagac catggttcca agttcccaat cgagcgacag
     9181 aagaggacac aattattttt ggtcattggt cggccttagg agtcgtaaaa ggaccacgcg
     9241 tgttcgcgat tgacggtgga tgcgtgtggg gacgggaact cattgccctg cgcctcgaag
     9301 accaacaact ctttcgcgtc acctgctcaa agatttaatc agatcatcct tcccgccgaa
     9361 ggatgaaagg ccaggcagtc tcccctttcc catacacgcc atatcccccc tgccgttccc
     9421 taaaaaaatt tacaccaccc attccatgta tctgatgaga tacattctgt atccatttag
     9481 atacctagtg attcctcctt cctctctgcc cttgggtaag cttcaatttt tattagcacc
     9541 atatagtgct taataattgt cggcatttac ttcctaggat ttattcaagt tcttcaggca
     9601 cttctcttgc ttgaatctct aggtaccacc gggaagcgta attgcgatgg aaagtaaaca
     9661 atcctgaaga cttaagggca cctcaccact gtatttattt taagaaaggg atcaacatga
     9721 tgaaatcaat tctccaacaa agaaccaatt catccgcgaa taattcggcc accacccaca
     9781 gaaaaattcg gcgacgccat actcgcatcc atttggattc ccttagaccc gatccatcgg
     9841 cctactgtgc cgacgcactc gctcaatgtc caaaatgcca aggcctggtg catcaagagg
     9901 gtggagaaac ttcgaatgaa accgtggtcc gctgtcttaa ttgtggatgg caaccgcatt
     9961 atcagactcc tataatacgt gaaacggccg aatccagatt gattcgctca cttaccgccc
    10021 agtttgtttc agaatacgag tggcatcgcg taccttaaag tttttacagc cacagtcatt
    10081 cggtgccagg gtgaccttgg gaatagttgc gaacaccgcg gtatttcctc cagtactcta
    10141 aagagtactt tcccattcca tctgagtaac caccctagac ggtttccggt ctagctcgct
    10201 tcctaggccc ccatccactt gaattcctgc tcatttctga ccaggaaatt tcccgacatt
    10261 ttgtgacgag ctttccctcg gtactaccct tgcttcttta caatatttgg gtgagactcc
    10321 atggtgaaag ttgcgtttgc gcctcaaccc gggaaacttt gagggaaaat aatatcgcac
    10381 agcatatact ggtgatgaat gaaatccccg attctttccc gcacaataag gaaagaatat
    10441 gaaggaagaa tctctatgac cagacactcg gcaatgtagg cacttattca tcctttccaa
    10501 tgaatcaaca atcaaccatt tcctggccat atggtagaac gaataacctc ataaacgtcc
    10561 ctgagacacg atgaaccatt tgagggcttt ttgccaccga tcagcctatt ggaggaaact
    10621 tgtccgggtg atcgtgtgtg tgggactttt cctcgacctg tccactgtca cctcggcgga
    10681 agaagtcgcc atactgaagt ccgcagacat cagcgcctat tcggaagcca ttaccgcatt
    10741 taaatccgcc cttcctccct ctctccacgt cacctcggaa tatgacctca aggatgacat
    10801 ggcagaaggc cggaacttgg cccgccgcat tcgcgcatcc aatgccaaag tcgtgttggc
    10861 cgtggggtta aaagcggctg tgaccgccaa actccaaatt cagaaaatcc ccgtcatatt
    10921 ttgtgtggtt attaacccga aaaaatatgg cttaccgaca gacaacatgg tcggcctctc
    10981 ttttcgtatc ccgtttcacc aacgcctgca aaccttacaa accctggttc ccaatgtctc
    11041 acgcatcggc gtgctcttcg atccagaaaa aacgaaaggg gtgttgaacc agcttctgca
    11101 agacgcggag gcgtcgggaa tcaggatcgt cacggaggaa gtatctgccg aaaaagatgt
    11161 ctcgaaagca ctcaatgcca tcaagaatcg catcgacgcc ttatggctcc tgccggacag
    11221 taccatccta aacgaaaaca ccctcgactt tttgctaagt acgaccttgg aagccaatgt
    11281 ccccctcctc ggcttctcgg ctgatttagt gcgcagcggc gccgtcgttg gaacgtactt
    11341 ccgttatgcg gacattggca aactggcagc tcagctatct caccagttgg cccggcagac
    11401 ttctccgtct caattgggaa ccatcatatc acccggacgt gttcggcgtt ccatcaatct
    11461 aagatccgca aggcatttgg gcttatccat cacttttgac ctcctaaggc agttcgatga
    11521 gcaattttaa cccgccacac gcccatccat cgccgcccct cccaactgaa ccggctggat
    11581 tggagccagg gtatctcacc ttgcgaacaa aattttcctt gttcgtcagc ctggttattg
    11641 ttctcgcctg ctcaagtctc agtggatatc tcatccaaca ggaagccgag gtcatggaaa
    11701 cctccttgct caatacgggt aaggttctgg ttaaaacact gcagaatata agcctcaatc
    11761 gattaatcaa ccaggatttg cactatctgg agagaatgct ggagggcgca atatccctat
    11821 cggaagtcgt ctacgccatc gcaagagacc aggacggcaa ggtgttagtc gaaaaatcaa
    11881 aagggaggtt agtggatatc acgaagttac gtcgtgacga aacgcagcct ttattcccgg
    11941 atgattccct cacggaaaca ctcatcacag cggcttccaa aatttccaaa attgaaccgc
    12001 tcgtgacggt cttatattcc tcacctcatc aaaagggact cctgcctaaa aggtctgcgg
    12061 aaaagaagtc tgcagaggga ttcatgtttg gatcagaaac catttatgac ttcgcccttc
    12121 c
//
LOCUS       DATIAL010000338         2785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_278704, whole genome shotgun sequence.
ACCESSION   DATIAL010000338 DATIAL010000000
VERSION     DATIAL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278704"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VLA60_10535"
     CDS             <1..646
                     /locus_tag="VLA60_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09843.1"
                     /translation="SNGISRSERFFHQLQSAVKSAGLQEQIPSAGIEIVSGGSCSLTV
                     LSPSRSQGARSNLFTTVSSGTDLNNQSLVTRFECGEHSFLLTADAEREALDGLLHTPN
                     SLSARVVKVPHHGARSSLNPEWVSRLQAEVMVISVGIHNRYGHPAQEVLDAYQRRGLP
                     LYRTDRDGAVWFTAALGSHDMTITTAKQKSLLTVQPGPHMLIEEIANWKRIWNW"
     gene            875..1603
                     /locus_tag="VLA60_10540"
     CDS             875..1603
                     /locus_tag="VLA60_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF09844.1"
                     /translation="MGNQEQLKLLKEGVGTWNAWRKANPSIRIDLSGGNLSGMDLPEV
                     DLDGANLHDVNFKNSNLSGSILSGANLMESSLMEANLTGANLKNANVNDADLLNVTLV
                     RANLERAHLAKTDMTGADISEANLTEANLHLANLHNAKFKRANLEGSKLREANMVQVD
                     LAGANLESADLAQSNLKQANISGANVENASLIQADLQEADLRDLHNMTLIQLSKARSL
                     RHARLDPPVEEQLAKDYPHLFRQN"
     gene            complement(1789..2550)
                     /locus_tag="VLA60_10545"
     CDS             complement(1789..2550)
                     /locus_tag="VLA60_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HSF09845.1"
                     /translation="METIGSLIGLGAVAGVFPIYLGIALALVIAKTMSRTWEGYLTGI
                     ATGVLVYLFFDLMHEAVEFTGATDLLSWGAFLGSLLIGFVGLVLIEQRQQGRYRAEPS
                     KLFLPYMIALGMGLHNLGEGLAIGAAYMQGEWVLSGVLIMGFALHNGTEGFAIIGSTG
                     KFRPGIKDIIMMGLLAGLPTCLGTLISGFAVSHYFTIIFFALAAGSLLYVIFSLTTIF
                     YTATRRVQSAYGVFTGISLMFVTAMVLQMVSGIRS"
BASE COUNT          775 a          631 c          711 g          668 t
ORIGIN      
        1 gagcaatggc atttcacggt cagaaagatt ttttcatcaa ctccaatccg cggtgaaatc
       61 tgccggcctc caggaacaga taccctcagc aggaattgaa attgttagtg gagggtcctg
      121 ttctctaacc gtgctgagtc cttcgcgcag tcaaggagca aggtcgaatc tcttcaccac
      181 tgtatccagt ggaacggatc tgaataatca atccctcgtc accagattcg agtgcgggga
      241 gcattcgttt ttattgacgg ccgatgcgga acgagaagcc ctggatgggt tgcttcacac
      301 tccgaacagt ctctcggctc gggtggtgaa agtgccgcat catggagcca ggagttccct
      361 gaatcctgag tgggtgagtc gacttcaggc tgaagtgatg gtcatttcag tcgggatcca
      421 caaccggtat gggcatccgg cccaggaagt gcttgatgcc tatcaacggc gagggcttcc
      481 cttgtaccgg acagatcgcg atggagcggt ctggtttacc gcagctcttg gctcgcatga
      541 tatgacgatt acaacagcga agcaaaaaag ccttctcact gtgcaacccg gccctcatat
      601 gttgatagag gaaatagcca attggaaacg gatatggaat tggtgaatct ggttttaact
      661 gtgctttgag cagaaagaac tgtttttttg atggtatgac cggtaaacct atccgggctt
      721 tggtctggtc gatccccccg ttcctgtcga ccactggaga caggttttct gcctgtggat
      781 agggctgaaa agtcttatcc ggttctgcta gaacaaaggg cgtgagtatc gggtaattgt
      841 ttctgtaaag ttagaaatgg agaaaaggag ttccatgggc aatcaagaac aactgaaatt
      901 actgaaagaa ggtgttggaa cctggaatgc atggagaaag gcaaatcctt cgattcgtat
      961 tgatttaagt ggaggaaatt tatcaggtat ggatttgccg gaagtcgatt tggatggcgc
     1021 gaatcttcat gatgttaatt ttaaaaactc gaatctttca ggatccatat tgtccggagc
     1081 caatctgatg gaatcaagtc tgatggaagc aaatctgacc ggggcgaatt taaaaaatgc
     1141 gaatgtgaat gatgcggatc ttctcaatgt cacacttgtg cgggcgaatc tggagcgtgc
     1201 gcatctggcc aaaacagaca tgacaggggc agatatctcg gaagcgaatt tgaccgaagc
     1261 caatcttcac ttggccaatt tacacaatgc caaattcaag agagccaatc tagaaggtag
     1321 caagcttcgt gaggcaaata tggttcaggt tgacctcgca ggagccaatc ttgagagtgc
     1381 tgaccttgcg cagtcgaatc tcaagcaggc gaatatcagt ggtgcgaatg tcgaaaacgc
     1441 ttccctgatt caggcggatt tgcaggaggc cgaccttcgg gatcttcaca atatgaccct
     1501 tattcaatta tccaaagccc ggtcgttgcg tcatgctcga ctcgatcccc ctgtcgaaga
     1561 acagcttgca aaagattatc cccatttatt ccggcagaac tgagaaaaag ccatggtggt
     1621 agaaattatc tcgtgtgagg agccatcgat aagggaaacc cggcagtctt cctgttacga
     1681 cgattgcaaa agaaatgaat aggtacaatt gggaaaaatc gaggggaatt cagggattca
     1741 ttctttgtcc ctcccgacgc gctgaaggga gttgaagaaa tgatgtattt acgagcggat
     1801 gccactgacc atctgcagga ccatggccgt gacgaacatg agactaatac cggtaaagac
     1861 gccataggcc gattggactc ggcgggtagc ggtataaaat attgtggtta gggaaaagat
     1921 gacatataaa agggacccgg cggccagcgc aaaaaatatg atggtgaaat aatgagatac
     1981 cgcgaatcca ctgattaatg ttcctaaaca tgtcgggagg cccgccaaca atcccatcat
     2041 gataatatct ttaatccccg gcctgaattt tcccgttgaa ccgataatgg caaatccttc
     2101 cgttccgtta tgtaaagcaa accccatgat cagaacccca cttaataccc attcaccctg
     2161 catatatgcg gcgccgattg caaggccttc acccaaatta tggaggccca ttcctaaagc
     2221 aatcatgtaa ggtaggaata gcttggatgg ctctgcccga taccgaccct gctggcgttg
     2281 ctcaatgagt accagcccca caaatccgat cagcaggcta ccgagaaagg cgccccacga
     2341 aagcagatcc gtcgcgcctg tgaattccac agcttcatgc atgaggtcaa aaaagaggta
     2401 cacgagaaca ccggtggcga ttccggttaa atatccttcc catgttcggc tcatggtttt
     2461 cgcgatgact aaggccaagg cgatgcccag atatataggg aacactcctg caaccgctcc
     2521 caaccctatt aaagatccaa ttgtttccat aaagtggttg aatctatcaa atatcagagt
     2581 ttattggcaa taatgaatat tggtttcatt ttcaaacatg aacaactata aatccgagat
     2641 catgaaatcc tggatgtatg gatgatttca aacagagcgc gaggttgaga gaacggctcc
     2701 catttcagga tggttaggga gtcgtccacg tagaccgggt ggcgaatgcc attgagcacg
     2761 caggtcacac caggggtgct ggcaa
//
LOCUS       DATIAL010000339         5044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1137423, whole genome shotgun sequence.
ACCESSION   DATIAL010000339 DATIAL010000000
VERSION     DATIAL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137423"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VLA60_10550"
     CDS             complement(<1..404)
                     /locus_tag="VLA60_10550"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HSF09846.1"
                     /translation="MAGISDIFNIARSGIRANQQGLATTSHNIANINTKGYSRQEVVL
                     ETARPAEGTIGSGVRVAAIRQSVDDFLENQLTSVNENLGSITARNNYLVQADGIFTET
                     DNSGLSVSLTEFFNAVRDLATNPESTIQRTVL"
     gene            complement(412..909)
                     /locus_tag="VLA60_10555"
     CDS             complement(412..909)
                     /locus_tag="VLA60_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HSF09847.1"
                     /translation="MIHPQSAMWERLLEMLAEAQSVFQRYGVLLFQEAHSLRIMDRPK
                     LVEVNGQKEQMLERMCQLEQQVERELHRLAGSAAYESIWSWLKAVPDPRAQLAQGMLT
                     DLLRLARLIQEQGEKNEALIRPIYHRVREAVQVMYTGFGTGPVYQGSGILHFPSVPSS
                     VHLQG"
     gene            complement(943..1263)
                     /locus_tag="VLA60_10560"
     CDS             complement(943..1263)
                     /locus_tag="VLA60_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HSF09848.1"
                     /translation="MTIRGLDPSGELGKLFFHVQAKNLQTQEASQPNATQRPSSADPV
                     DLSVLAQEIRDYSTRVAQVPELREEKIKRIKEVLEAGGQLATSSQVGDALTRETILNE
                     LGSK"
     gene            complement(1325..1681)
                     /locus_tag="VLA60_10565"
     CDS             complement(1325..1681)
                     /locus_tag="VLA60_10565"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HSF09849.1"
                     /translation="MIADMFPMDMTGLSHSVTTGRIDRVEDSVRRGELLRASQEFESY
                     FLSYLMKVMRETVPKGEFTANPMGEMYYSFYDEEIGKRAAQAGGIGLSAYVLDTVARN
                     EDLTSSPTSGPPFLKS"
     gene            complement(1739..2953)
                     /locus_tag="VLA60_10570"
     CDS             complement(1739..2953)
                     /locus_tag="VLA60_10570"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HSF09850.1"
                     /translation="MNRPTYFRNGRKPLRKSGQAWVSGWGVVLVLIGGCLMVSASAVI
                     LPGEIQAARIKDIASIEGVRENQLVGYGLIVGLDRTGDSVVGGQFTAQAIISMLNTMG
                     LKLNIDPIQLLTKNTASVMVTAKLPPFARPGMKLDVQVSSMANAKSLRGGTLLLTPLK
                     AANQQVYAVAQGPLSTSGFEAGAAGTTVKKNQQSGGIIPGGAIVERAVDVDMQAWEKF
                     SLTMHQADFITALRVSEAINAHLGGGVASAVNSGQIHVGIPERFQGKIVQMIAAVEGL
                     DVNVDVLAKVVVNERTGTIVMGEHVRLARMAISHGDLTIKVGTQFNVSQPEAAFLGRG
                     AQGAGRTVVTPDVQTDVQEEDGRVIQVDSSVTLGDLVKALNSLGVTPLDLVAVLTAAK
                     AAGALQADLELI"
     gene            complement(3002..3703)
                     /locus_tag="VLA60_10575"
     CDS             complement(3002..3703)
                     /locus_tag="VLA60_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HSF09851.1"
                     /translation="MSNNVLRAKSLAWGVLLLVVVFGGVGCAEEKTIRQVEAESNLLE
                     KSPPSSMGSLWDPGNGRAYMFEDRRAGRIGDIVVVQIVEQHKGSKKANTKADRDSSLS
                     AGVSGGLFGINTLSQKFAEYFNIDAGTSHEFEGDGSTSREDTLTGTIAANVIEVFPNG
                     DLRIRGKREVTVNSEKQTMTIKGIVRRIDLDTQNMVLSSKVADAEISYTGLGVVDDVQ
                     RPGWATRIFDWLTPF"
     gene            complement(3700..4443)
                     /gene="flgA"
                     /locus_tag="VLA60_10580"
     CDS             complement(3700..4443)
                     /gene="flgA"
                     /locus_tag="VLA60_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HSF09852.1"
                     /translation="MIFLGLFLCGNGLGTLGGMSWAEGPVSSTPVRPDSQSLDGRDFE
                     RVILAELKRRYGRSQHQVSFRVLFPKQGIEVPNGKIHLEIGDWSGGGRTGRRAFRVGI
                     FVNQQFLRTVNVVGEVKAQVEVATPIRWLKPKEVVTGEDLSVMLVDAPSLTHDFILDL
                     DEVIGKQVLRPLPPRQPIRKIMLDYPPMIHKGDRVMIEVRGDGLLVQTVGIAKAAGKK
                     GETIPVKNQTSGREVLATVLASGLVEVGF"
     gene            complement(4467..>5044)
                     /locus_tag="VLA60_10585"
     CDS             complement(4467..>5044)
                     /locus_tag="VLA60_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HSF09853.1"
                     /translation="RPVTVAKEYVQGSLKQTTNPLDLAIEGVGFFQVTRPDGTTMYTR
                     AGSFKQDSTGNVVSGDGDQLIPSITIPSGALQVDIGQDGTVSALLPGVSQATQVGQIQ
                     LVRFDNPSGLIAQGNNLFSESSSSGPAQQGTPGFSTGFGNIQQGFLEISNVNIADEMV
                     NMIIAQRAYELNAKAIQASDDMMQVVNGLRR"
BASE COUNT         1239 a         1491 c         1075 g         1239 t
ORIGIN      
        1 aacaggaccg tccgctgtat ggtactttct ggattcgtgg ccaaatcccg aaccgcattg
       61 aaaaactctg tcagactaac cgatagtccg gaattatccg tttcggtaaa aatcccatcc
      121 gcttgaacca agtaattatt cctggcggtt atagatccaa gattctcatt gacggaagtc
      181 aactggtttt ccagaaaatc atcgacggac tgacgaatag cagcaacccg gactccgctt
      241 ccaatcgtcc cttcagccgg ccgcgcagtc tcaaggacga cttcttgtct ggaatagcct
      301 ttggtattaa tattggcgat attatgggac gtggtcgcga gcccttgttg gttggcacga
      361 ataccggaac gcgcgatatt aaagatgtca ctgatgcccg ccataagact cctatccctg
      421 gagatgaacg gagctaggaa ccgaaggaaa atgtaatatt ccagatccct gatacaccgg
      481 accggtaccg aaccctgtat acataacctg aactgcctca cggaccctat gatagattgg
      541 gcgaatcaga gcttcattct tttccccttg ttcctggatc aaacgcgcca gccgaagaag
      601 gtccgtcaac attccttgcg ccaattgtgc ccgtgggtcc ggaacggctt tcaaccaact
      661 ccaaattgac tcgtatgcgg ccgacccggc caaccggtga agctcacgct ctacctgctg
      721 ctccaactga cacatcctct ccaacatttg ttccttctgc ccgtttacct cgaccaactt
      781 aggccgatcc atgatccgaa ggctgtgcgc ttcttgaaaa agcaacacac cataccgctg
      841 aaacaccgat tgggcttcag ccaacatttc cagcaaccgt tcccacattg ccgattgggg
      901 atgaatcatc ggtcaggcat ttctcttata aatgtcaggt gtttattttg agcccagctc
      961 gttaagaatc gtctctcggg tcaaggcatc acccacctga ctggaggttg ccaattgtcc
     1021 acccgcctcc aatacctcct taatacgctt gattttctcc tctctcagct caggaacctg
     1081 cgccactcga gtagaatagt cgcgtatttc ctgcgccaac acggaaagat caaccggatc
     1141 agcagacgaa ggacgctggg tggcgtttgg ttggctggcc tcctgcgtct gaagattttt
     1201 agcttggacg tgaaaaaata gtttccccag ctctccactg ggatcaaggc cccgtatggt
     1261 catggcgtca tgcctccttc actgtatgta tcggaagcaa cgccgttaaa ctttacagaa
     1321 cctcttaaga tttcaaaaaa gggggacctg aggtgggaga actcgtgagg tcttcattcc
     1381 tggccaccgt gtccaacaca tacgcagaaa ggccaatccc tcccgcctga gcggcacgtt
     1441 tgccaatttc ttcgtcataa aaggaataat acatttcccc catggggttg gctgtgaact
     1501 cgcctttggg gaccgtctct cgcatcacct tcatcagata ggacaaaaaa taggattcga
     1561 attcctgact cgcacggagt aattctcccc gacgaacgga atcttccacc cgatctatcc
     1621 tcccagtggt gacggagtgt gagagacccg tcatatccat agggaacatg tcagctatca
     1681 tagaccacat cctttccgcc agacagtcat caacaacgtg ctctcttcag atcctgacct
     1741 agatcaactc caagtcagcc tgaagcgcac ctgcagcctt tgccgccgtt agtacggcga
     1801 ccaaatcgag aggagtaaca cctaatgaat tcaacgcctt gaccaaatcc ccaagcgtca
     1861 cggaactgtc tacttgaatg actcgaccat cctcttcttg cacatcggtt tggacatccg
     1921 gagtcactac agttctaccg gcaccctgtg ctccccgccc aagaaaggca gcctctggtt
     1981 gggagacatt aaactgcgtt cccaccttga tcgtgagatc gccatgggaa atggccatcc
     2041 tcgccaaccg gacatgttca cccatgacaa tggttcccgt tcgttcgttc accacaacct
     2101 tggccaggac gtccacattc acatccagcc cttcaaccgc agcaatcatt tggactatct
     2161 tgccctggaa ccgttccgga atccccacgt gaatctgtcc ggaattgaca gccgaggcca
     2221 caccaccccc aagatgagca ttaatggctt cagacacacg caatgccgtg atgaaatcag
     2281 cctgatgcat ggtcaaagaa aatttctccc acgcttgcat gtccacatcc accgctcgtt
     2341 cgacaatcgc tccaccagga ataatccccc cggattgttg atttttcttc actgtggtcc
     2401 cggcagcacc agcttcaaat cccgatgtgg agagcggccc ttgggcgaca gcatacacct
     2461 gttgattggc tgctttcagc ggcgtcaaca acaacgttcc ccctcgcaaa cttttggcat
     2521 tggccatgga ggagacctgc acatccaatt tcatgccggg tctcgcgaac ggcggcaatt
     2581 tggcggtcac catcacagac gcggtatttt tagttaataa ttgaatggga tcaatattga
     2641 gtttgagccc catggtattc aacatggaaa taatggcttg cgccgtaaat tgaccaccga
     2701 cgacggaatc accggtccgg tccagaccaa caatcaagcc atacccgact aactggtttt
     2761 cacgcacgcc ttcaatggag gcgatatctt tgatgcgggc cgcctgaatt tctcccggta
     2821 aaatgaccgc actggcactg accatgaggc atccgccgat caaaaccaag acgacacccc
     2881 atccggaaac ccatgcttgt ccggatttcc ttaaaggttt tctgccattt ctaaaatatg
     2941 ttggacgatt caccacattc tccaattagc tcaaaaaata atgaactcat ccccaaataa
     3001 cttaaaacgg cgtcaaccag tcaaaaatcc tggtggccca ccccggtctc tgaacatcgt
     3061 ccaccactcc taatccggta taggaaattt ctgcatcggc aactttggat gacaagacca
     3121 tgttctgagt atccagatcg atccggcgga caatgccctt tatggtcatt gtttgctttt
     3181 cactattaac cgtgacctcc cgtttccccc gaatccggag gtcaccgttg ggaaagactt
     3241 caatgacatt ggcggcaatg gtgcccgtca aggtatcttc ccggctcgtc gatccgtcgc
     3301 cctcaaattc atgggaagtc ccagcatcaa tattaaaata ttctgcaaac ttctggctga
     3361 gggtgttaat cccgaacagt cccccgctta ctccagctga caggctggaa tcccggtctg
     3421 ctttcgtgtt ggcttttttg gagcccttgt gttgttcgac aatttgcacg acaacaatgt
     3481 ccccgattcg acccgctctc ctatcttcaa acatgtacgc tcggccgttt cctgggtccc
     3541 agagggatcc catggaggat ggcggactct tttcaagaag attcgattca gcttcgacct
     3601 gtctaatcgt tttttcctcg gcgcatccta ccccgccaaa aacgaccacc aggagcaaga
     3661 ctccccacgc taaagacttc gccctcaaca cgttattgct caaaatccaa cctccacgag
     3721 tcccgatgcc aaaacggtag ctagaacctc ccggcccgat gtctgattct tgacaggaat
     3781 ggtttctcct tttttaccag ccgctttggc tattcctacg gtttgaacca gcaaaccatc
     3841 acccctgacc tcaatcatca cccgatcgcc tttatgaatc atcggcggat agtccaacat
     3901 aatttttcga atcggttgtc ttggtggtaa tggacgtaag acctgcttcc ctatcacttc
     3961 gtcaagatca agaataaaat catgtgtcag tgaaggggca tccaccagca tcacagacag
     4021 gtcctcgccg gtcacaacct ctttgggttt caaccagcgt attggtgtcg ccacttcgac
     4081 ttgggccttc acttccccca caacattcac cgttcggaga aattgctgat tcacaaaaat
     4141 ccccactcga aaggcacgtc gtcctgtcct cccgcccccc gaccaatctc caatctcaag
     4201 gtggattttt ccgttgggga cctcaattcc ttgcttggga aacaataccc gaaacgacac
     4261 ctggtgctgc gaacgtccat agcggcgctt cagttccgcc aggatcaccc gttcgaaatc
     4321 tcttccatcc aacgactgac tatccggacg aacaggtgtt gatgagactg gtccttccgc
     4381 ccaggacatt cctccaagtg tccccaaccc attcccgcac aggaacaatc ccagaaatat
     4441 catgatgatt ttcaatatct taccctctaa cgtctgagtc cgttaacaac ctgcatcata
     4501 tcgtccgatg cctgaatggc tttcgcattc agttcatagg cccgttgggc aatgatcata
     4561 ttgaccattt catcggcaat attcacatta gatatttcaa gaaatccttg ctgtatattt
     4621 ccaaaaccgg ttgaaaatcc tggcgttccc tgttgtgcag gacctgaaga cgaactctct
     4681 gaaaataaat tattgccttg cgcaatcaag ccagacggat tgtcaaatcg gaccaattgg
     4741 atttgcccaa cctgagtggc ttgggacacc ccgggcagca acgcggacac cgttccatcc
     4801 tgcccaatat ccacttggag tgctccggac ggaatggtaa tgctcgggat gagctgatcg
     4861 ccgtcacccg aaaccacatt acccgtactg tcttgtttaa aggatccagc ccgtgtatac
     4921 atcgtggtcc catccgggcg tgtgacttga aaaaatccca ccccttctat cgccaaatcc
     4981 aacggattcg tggtttgctt gagacttcct tgcacatact ccttcgccac ggttaccggc
     5041 ctca
//
LOCUS       DATIAL010000340         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1352720, whole genome shotgun sequence.
ACCESSION   DATIAL010000340 DATIAL010000000
VERSION     DATIAL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352720"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /gene="secD"
                     /locus_tag="VLA60_10590"
     CDS             complement(<1..1501)
                     /gene="secD"
                     /locus_tag="VLA60_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HSF09854.1"
                     /translation="MKKLRGRLLLLLVVTVGSVILAIPSFPALYQSLPDGIKGILSHR
                     GLSLGLDLQGGIHLVLEVEEERAVEIAVDRSRKAMEDLLKEKAMAVEGIHREGSYTIV
                     ITLQQEAKGEEIRKLLDEAFPNFESQGQSGTRLVYELRSTEVERVKTSAINQALETLR
                     NRIDQFGVAEPLIQRLGLNQIAIQLPGVKDPQRAKDLIQETALLEFKLLEESKVALDL
                     PPQVAKGLEDKIRQDLEGKIPEGSEILFESAISESDGHSYSIPYLVKKDAALIGDVLQ
                     DARVTIGDFNEPIVSITFDSKGAREFDLLTASNIGKRMAVVLDGKVYSAPVIRDRISG
                     GRAVIEGTFTTAEANDLAVVLRAGALPAPLKTLQDLTVGPSLGQDSIEKGLRTTIIAG
                     TLVLIFMIVYYRLSGFIANMAVFLNLVCLLGALSGLNATLTLPGIAGIILTIGMGVDS
                     NVLIFERIREELRQGRPVRLAVDSGYDKAFLTIVDSHVTTLITGLALFLF"
     gene            complement(1521..1844)
                     /gene="yajC"
                     /locus_tag="VLA60_10595"
     CDS             complement(1521..1844)
                     /gene="yajC"
                     /locus_tag="VLA60_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HSF09855.1"
                     /translation="MNLDSYAWAQAGGASSDSSAGLLSLIPFLLIFVVFYFLLILPQQ
                     RRQKKQRELLDGLKKGDKVITSSGIWGTVTNLDKETATLQIADNTKIRLQRDHIASIR
                     TSNES"
     gene            complement(1884..>3049)
                     /gene="tgt"
                     /locus_tag="VLA60_10600"
     CDS             complement(1884..>3049)
                     /gene="tgt"
                     /locus_tag="VLA60_10600"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HSF09856.1"
                     /translation="MTPTSKPNRFDVHKSSGSSRARVGLVSTLHGEIETPAFMPVGSQ
                     GTVKGLDPKEVRECGFDLILANAYHLFLRPGHELIESHGGLHAFMQWPGAILTDSGGY
                     QMVSLANLCEVTEEGVGFRSHLDGEWHLLSPEKSIGIQVALGSDIMMVLDHCPTFPST
                     ELQARESVQRTTRWAKRCAVIPRKSHQWLFGIVQGGMFPHLRQESAQELVNMNMDGYA
                     LGGLSLGEEKSAMMDIIETVVEWLPSIRPRYLMGVGLPEDIVEGVARGLDLFDCVIPT
                     RHGRTGWLFTSTGRVLIKQACYARDPNPIEAECGCPVCQRFSRAYLRHLFLSHEMLGV
                     RLNTVHNLWYYGSLMKEIRQAIRENLFETFRKEFHYRRHDDRDKTFLQEMPTY"
BASE COUNT          843 a          813 c          639 g          754 t
ORIGIN      
        1 caaataaaaa gagtgccaat ccggttatta aggtggtgac atgggaatcc acaatcgtca
       61 agaaagcttt gtcataacca ctatccactg ccaaacgcac gggacgaccc tgccgcagct
      121 cttctctgat ccgttcaaaa attaacacat tggaatcgac tcccattcca atagtcaaaa
      181 taataccggc aataccgggt aaggttaggg tggcattgag tcctgataag gctcctaata
      241 aacaaaccaa attgagaaat actgccatat tagcgatgaa cccggagagc cggtaataca
      301 caatcatgaa gatcaagact agagtccccg caatgatggt cgttcgtaaa cccttctcaa
      361 tcgaatcctg tcccagagaa ggtccaaccg ttaaatcctg caaagttttt aatggtgccg
      421 gaagagcacc agcccggagt actacagcca gatcatttgc ctctgccgtc gtaaacgttc
      481 cttcaatgac cgcccgccct ccactaatgc gatctcgaat aaccggcgca gaatatacct
      541 tcccatccaa caccacagcc atccgcttcc caatattgga agccgtcaac aaatcaaatt
      601 cccgtgcccc tttgctgtca aaagttatac tgacaatggg ctcattaaaa tcaccaattg
      661 tcacacgtgc atcctgcagg acatcgccaa tgagagccgc atcttttttc acgagataag
      721 ggatgctata cgagtgccca tcggattcag agatcgcaga ttcaaagagg atttctgacc
      781 cttctggaat tttaccttcc aaatcctgcc tgattttatc ttctagccct tttgccacct
      841 gtggaggcaa atccaaggca accttcgatt cttccaataa tttgaattcc aataatgccg
      901 tttcttggat aagatctttg gctcgctgag gatctttgac ccctggcagt tgaatcgcaa
      961 tttgattaag ccccaaccgc tgaatcagag gttcggcgac cccaaattga tcaatacggt
     1021 ttcgtagggt ttcaagagcc tgattgatag ctgaggtttt gactcgctcc acttcggtgg
     1081 agcggagctc gtataccaaa cgagttccgg attgaccttg agattcgaaa ttcggaaatg
     1141 cctcatcaag gagctttcga atttcttcgc ccttggcttc ttgttggagg gtgatgacaa
     1201 tcgtgtacga gccttcacga tggatgccct ctaccgccat cgctttttcc ttcagtaaat
     1261 cctccatggc ttttctggac cgatcgacgg cgatctctac cgcccgttct tcttcgactt
     1321 ccaacaccaa atggattcct ccttgaagat ccaaacccaa ggagagccca cgatgactta
     1381 aaatcccctt tataccatca ggcaaagact gatacagcgc tggaaacgaa ggaatggcca
     1441 ggatgacaga tcccaccgtg accacgagaa ggagaagtaa ccttcctcga agttttttca
     1501 ttcatctacc ccttgataca tcatgattcg ttcgacgtgc gaatgctcgc gatatgatca
     1561 cgttgcaacc gaatttttgt attatcagca atttgtagcg tggcagtttc tttgtccaaa
     1621 ttggtgaccg tcccccagat tccagaagac gtaatcactt tatcgccttt tttcaggcca
     1681 tccagtaatt cccgttgttt tttctgccga cgttgctgtg gcaaaatgag caaaaaataa
     1741 aacaccacaa aaatcaatag aaacggaata agggatagta atcccgccga ggaatcggaa
     1801 ctggctcccc cggcttgagc ccacgcatag gaatctaaat tcatagctac ctcaattaaa
     1861 aaacatacaa ctggtaaatg gattcaatag gttggcattt cctgaaggaa cgtcttatct
     1921 cgatcatcgt ggcgtcgata gtgaaattct ttccgaaagg tttcgaacag attctctctg
     1981 atcgcttgcc gaatctcttt cataagcgag ccataatacc agagattatg taccgtattc
     2041 aggcgaacgc ccaacatttc atgagacaag aataaatgcc gcaaatatgc cctggaaaat
     2101 cgttgacaca ccggacaccc gcattcggcc tctatgggat tgggatccct ggcatagcag
     2161 gcttgtttga tcaacaccct ccccgtcgag gtaaacaacc agccggttcg tccgtggcgg
     2221 gtcggaatca cgcagtcaaa taaatccaac cctcgagcca ctccttccac aatatcttcg
     2281 ggaagcccaa cacccattaa atagcgggga cggatcgagg gaagccactc aacgactgtc
     2341 tcgataatat ccatcatcgc ggacttttcc tctcccaagg ataatccgcc tagggcatac
     2401 ccatccatat tcatgttaac caattcttga gccgattctt gtctcaaatg aggaaacatt
     2461 ccaccctgaa caatcccgaa aagccactgg tgggattttc tcggtattac ggcacaccgc
     2521 ttggcccagc gggttgttcg ctgaacggac tcacgggctt gcaattcagt gcttggaaag
     2581 gtggggcaat gatcgagaac catcataatg tccgatccaa gagcaacttg aatgccgata
     2641 cttttttcag gtgagagcaa atgccactca ccatcgaggt gagaacgaaa accgactccc
     2701 tcttccgtca cctcacagag atttgccaag ctcaccatct gatatccgcc gctatcggtc
     2761 aaaatggcac caggccattg cataaaggca tggagccccc catgagattc aatcaattca
     2821 tggccaggtc gtaaaaagag gtgataggca ttagccaaaa ttaaatcaaa cccacattca
     2881 cggacttctt tcggatccaa accctttacg gtcccctgtg acccaaccgg cataaatgcc
     2941 ggagtttcga tctctccatg aagggtggat accagtccga cccgcgcgcg tgacgaaccg
     3001 gaggatttgt gaacatcaaa acgattcggc tttgacgttg gtgtcattt
//
LOCUS       DATIAL010000341         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1245639, whole genome shotgun sequence.
ACCESSION   DATIAL010000341 DATIAL010000000
VERSION     DATIAL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245639"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            950..1453
                     /locus_tag="VLA60_10605"
     CDS             950..1453
                     /locus_tag="VLA60_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF09857.1"
                     /translation="MRLSKKSEYALRALSELTQEFGQGMVQRKTLADRQNIPLGFLEM
                     ILLSLKNAGLIGSRRGAHGGVYLIKSPGEITLGQIIRLLDGPLAPIACVSQTAYQKCQ
                     DCPYATTATCPIQHIMLDVRNAIASVLDHYSLEDFVLSRKDPSSTNTGTRRELRTRHI
                     KEPVKRP"
     gene            1493..1714
                     /locus_tag="VLA60_10610"
     CDS             1493..1714
                     /locus_tag="VLA60_10610"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HSF09858.1"
                     /translation="METHWRSVVKGISWRAVGTLDTTVLVFIFSGELLLAASVGLTEL
                     ITKIFLYWAHERVWQRIRWGRIFPSTSSP"
     gene            1749..2156
                     /locus_tag="VLA60_10615"
     CDS             1749..2156
                     /locus_tag="VLA60_10615"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HSF09859.1"
                     /translation="MAQRILISGALMALLGVAVGAFGAHGLRPLLSEPMLAVFETGVR
                     YQVFHALGMLIAGLSLAYAPTRYFRFAAWTFFLGIILFSGSLYVLSVSGIRALGIITP
                     FGGLCFIVGWGLLAWGYWKSVPSKNPPKSPPSG"
     gene            complement(2172..>2626)
                     /locus_tag="VLA60_10620"
     CDS             complement(2172..>2626)
                     /locus_tag="VLA60_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF09860.1"
                     /translation="ITLRDLSGTVHIFPNGTVTTLSNMSKDWSYYLLDIGVAYKEDTD
                     RVSAVMKEVGRELQEDPQIGPNILEPIEILGVDQFGDSAVVMRARLKTLPIQQWMVGR
                     EYRRRLKKAFDKEGIEIPFPHRTLYMGEASPPFTIKGSLAQAEMESPS"
BASE COUNT          636 a          660 c          635 g          695 t
ORIGIN      
        1 cgcgacgcaa aagtaccagg atctgcagat atccaaaccg cggaggggcc taagcgggat
       61 gacgaatccg gatgtccaac gccgattgat ccttggccct gcttcgcttt tggcgaaggg
      121 taaaaagact tgctccttct ctcgagatca accgaccaac gggctgtgag aaaaaaagat
      181 gaaagtcacc cgccttaggc gaggcccaaa gcgggtgacc aaatccctac tgtaaacgat
      241 aacaaacgag aaaagccgta acatcccaat cccggttatt gcggttccgc accccgattc
      301 tcatggtcct tgttttggag tatcaaacca ctgtcataca ggtatctcgg actaaaggca
      361 aagaaaccgg gtggacacgt caggacgtga accgcaatac catcgctaac gccgtcgtta
      421 actgggacgg taaattgttc caccactcct tgatgttaca actatgcgcc ctttcccccg
      481 ccggcctcag tggcccttga ggccctgttt gagcatggcc aagaaaattc tcgcgacccc
      541 attatcgtca ccacgaccca tcccaatcat gctgcagact tgcctcaaat gaggtctccc
      601 cccaaaaagg acagctacac tgtcacaaga tatgagtatt gaagaccgtt tataattgtt
      661 gagcactctt ttcgaaatcg tccgccaaac gtgtccaaga ccaaccgcgt gaaggtcgtg
      721 actgggtttc ctcatcccga atggttttcc ctttaacccc cgcaggcttt ccggctgaat
      781 tatccgatta cccttcttgc caatctctcg tgacgttacc ccgtgcagaa attctcatgg
      841 ttgaggaggt actctccgtc gaagtgtggc ccaacttcct cgtttgggct ggaaacttga
      901 aataaattat ggtaaatgtg aataaaatta gttaactttc tagactatca tgcgactttc
      961 gaagaaaagc gagtatgcct tacgggccct tagtgaactc actcaggaat ttggtcaggg
     1021 gatggtgcaa cgcaaaacgt tggcagatcg gcaaaatatt ccattaggtt ttcttgaaat
     1081 gatcctgttg tcgttaaaaa atgctgggct cattgggagt cgccgcggag cccatggggg
     1141 ggtgtattta attaaatcgc caggtgaaat taccttggga caaattattc gactgttgga
     1201 cggccccctc gcgccgattg cctgtgtaag tcaaacagcc tatcaaaaat gccaggattg
     1261 tccttatgcc acgacggcca cgtgtccgat acaacacatt atgctggatg tacgaaatgc
     1321 cattgcgtct gtcctcgatc attattctct ggaagatttc gtattatccc ggaaggatcc
     1381 gtcatcaacg aacactggaa cacggcggga gttgagaacg agacatatca aggagccggt
     1441 taaacgtcct taggagaatt tttaccgtat gagggaattt taagaggaga atatggaaac
     1501 tcactggcgt agcgtagtga agggaatcag ttggcgtgct gtcggcacgt tggacaccac
     1561 cgtgttagtg tttatttttt caggcgaact tcttcttgct gcttctgttg gactgacgga
     1621 attgatcacg aaaatatttt tgtattgggc tcatgagcgg gtctggcaaa ggattcgatg
     1681 ggggcgaata tttccttcaa ccagttctcc ataattgtaa ccttctctca accgatccca
     1741 tgcacgatat ggcacaacgg atactcatca gcggggcgct tatggctctt cttggcgtag
     1801 cggtgggtgc gtttggcgcg catggactgc gacccttgct ttcggaaccc atgctcgccg
     1861 tctttgaaac aggtgtccgg tatcaggtat ttcatgcgct gggaatgctc attgccgggt
     1921 tgagcctggc gtatgccccc actcgctatt ttcgatttgc cgcctggact tttttcctgg
     1981 gcattatatt gttttcagga agtctctatg tcctatccgt ttccggtatt cgggcgttgg
     2041 gtatcatcac accctttgga ggattgtgct ttattgtagg atgggggttg cttgcctggg
     2101 gatattggaa atctgtcccc tcgaaaaatc ctccaaaatc cccgccatcc ggctaaaagc
     2161 atcttttaat atcatgaagg ggattccatc tctgcctggg ccagagagcc tttaatggtg
     2221 aagggtgggc tggcctctcc catgtacaag gtccgatggg gaaaaggaat ttcaataccc
     2281 tccttatcaa aagccttttt cagccgccgc cggtattctc tccctaccat ccattgttga
     2341 atggggagcg ttttcaatct ggctctcatg accaccgccg aatcaccaaa ctgatccacc
     2401 cccagaattt caatgggttc aagaatattt ggtcctattt ggggatcttc ttgtaattcc
     2461 ctgcctacct ccttcatgac ggctgacacg cgatcggtat cttctttata cgcgacgccg
     2521 atatccagaa gataataaga ccaatctttc gacatattgg agagggtggt cacggttcca
     2581 ttcggaaaaa tgtgcactgt tcccgacagg tcccgaagag taatcg
//
LOCUS       DATIAL010000342         7907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1030105, whole genome shotgun sequence.
ACCESSION   DATIAL010000342 DATIAL010000000
VERSION     DATIAL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030105"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VLA60_10625"
     CDS             complement(<1..240)
                     /locus_tag="VLA60_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09861.1"
                     /translation="MKIFLFDLVGNQRINGNNEPPSWMPAYSMGTGDVQNPFSPSFLP
                     VTMAFIKGKQRYPLDEYKEIQPFLGHIPRKRPPHSR"
     gene            239..598
                     /locus_tag="VLA60_10630"
     CDS             239..598
                     /locus_tag="VLA60_10630"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSF09862.1"
                     /translation="MDGTSVAIIASGVLVGMGASFTGLGGGFLMVPLLLFLGFTAQKA
                     VGTSFVGILVIAVSALIAHGRMAHVDYKYGLLLGLGGIIGAQIGARLVEGVPTDMFKK
                     IFAVILIGLAIYLFVKK"
     gene            947..2923
                     /locus_tag="VLA60_10635"
     CDS             947..2923
                     /locus_tag="VLA60_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HSF09863.1"
                     /translation="MIEPIFLQSLFLLGTAVVVVVGFHRLHIPSPLAYLLVGVLLGSY
                     TPGPVIEAQPVRALAEFGIVFLLFTIGLNFSMPQIHALRHQLLGLGTGQVLLTTIAVG
                     LLAWLVDLPIAAAFVVGAVFAQSSTTIISKQLIEQGEEHSRHGRLGIAMSVFQDVTAV
                     PFVVVIPVLAMANTEVVVLAGSLGMAFAKAALAFGLVFLAGRWVMRPLFYLVAELRST
                     EVFTLTVLFVSLVSAWTTSSLGLSMAFGAFLAGMMLGETEFRHQVESTIRPFRDVLLG
                     LFFIGIGMLVDPSSIPGIWHWALMGATVLLLIKAALVALMVRLSGMDTLTAWRTGWLL
                     AVGGEFGFALLSIALDVGVIQAKIGQIVLTSVLFSMIVAPFLIRYNQVLARWCAPRPA
                     REGKDAVPQVRIDTLGPLRDHVIICGYGRIGQSVAHLLEEEKIPYVALDLDSSRVKDA
                     NIAGEGVFYGDASERTILEGVGVGTARLVVIAHADVASAHKTLHHLRALRPDLPIMVR
                     TRDETHVDELRAAGATEVVPETLEAGLMIAAHALVLLNVPISRVARRMREQRNGRYHL
                     LREFFLGDSLLAYTRQEHNVDRLRPVVLPPDSSFVGRTLHELNLNGVAIAALVREGQR
                     QLMPSGETRLEAWDVVVLFGSPDDLQRAERALLE"
     gene            3149..3490
                     /locus_tag="VLA60_10640"
     CDS             3149..3490
                     /locus_tag="VLA60_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09864.1"
                     /translation="MATWLIPALKAVLPHIGTIISATAPAFTKKGTAPTTDQTHLLQQ
                     QISELQTAASQNATHVKELAEQLQRTVAAIEQAASVATSNQQRALQLSLAAIALSAIS
                     LCAVLLHVLFL"
     gene            complement(3914..>7907)
                     /locus_tag="VLA60_10645"
     CDS             complement(3914..>7907)
                     /locus_tag="VLA60_10645"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HSF09865.1"
                     /translation="SLPGGAVQHNITGGTRPGGGGNLFHSFGDFGVPTDNIANFLNDT
                     GLPTSNILSRVTGGNPSNIFGTIQTEGFGNANFFLMNPAGIVFGPNASLNVGGATHFT
                     TADYVRLADGAQFTALPGAQDSLLSIAPIAAFGFLGPNPAGISVEGSTLSVSEGQTLS
                     LVGGDITIGRSNLTAPGGQIALASVGSPGEIQAHTLDKTSNIHRQTIESGGTIRISDQ
                     SGMDAGGEHGGTVLIRGGRLVLDHDSQIRANTLGGQDGGHVDIEVSENVLVAGTGVQT
                     DTPSGIFARTEGSGKGGTITISAQTVELTDGGHMSVTTRGAGDAGTIMVTAPDGEVIL
                     SGSDNRLTDFSPGEEAVSGLFANTVGTGQAGTITVQTGNLTISEGARISAGTVGRGSG
                     DAGKIFITATGDVLLSGTHANGIASSGLFAGASELTFPGNGILGSGGTINVTGQTVRI
                     LDGAKIHVASIGQEQPDMHMLDNAPSAGTVNIKAATLELTGADMDGNVIDIIPSEMTA
                     SATGPASAGHILLEADNIRIEDGGVVTSETESITKIEKTGSIRIHAEEDLVISGTAIR
                     TEFPPNLLPQNTLNPRTVVPSALRAHTKGVNRGGDIQISAENVSVTGGGLITNAALDG
                     AGAGGSIHLDVVDTIRLSGAYEKVDKNRTIFRTEISSGTQGPGHGGDITITAKNVEML
                     DGAWMSSRTRGRGSGNAGRVSITAEENVSLTGFMSVYDPEINNTSKTQEISSRIIARA
                     EEHDNRLADLPLGNAGDISITAKHVVMKDGAEIIAPSSSGRVIPITDAIEKIVANMPK
                     EQRPRAGNITVNADTIILSGARTVGGSGVFGWAPTTIRAGVIGPAGTGNISLTANTIV
                     IRDGADVTAESQSGTPGVQRGNVTITAHENLFLSGYLLRTEFPENPIGSDPFPEANGR
                     TIRPSSIGTRTHGINQAGDIIISAKNLTIANGAQITNGTTNLQPENAGPGGKILLTAD
                     HLTMSGMAPFPDAIGRPLPSIISSRTDGAGDAGQIEVMSKTIEIKSGGQISANSIPRE
                     GAIDPGAGGNIQLMATESFRAEGVGILEGTPVVRSGLFSSTEGPGLGGNLQVTSPVFE
                     MKNGAALSASTTGTGNAGDILITAGDSILLDKASVTTEAGSAVGGNIKLTADNRIDLT
                     NSRVTSRVKEGSGSAGSINFDPDFIVLKNSQVLSTAVSGSGGDITLTAKSAIVMDPFT
                     FLDARSQFGGSGRVNIESPVQFLSSTIVPLPQNLVPVTTLYGTKCGGEKGRFSSFVDS
                     KADTIAPTPGTLLASPLLPFASHSGVLNPIDLHVGSNNQTQAAPIHVAAYSPPVLFGQ
                     TTGQRATCRP"
BASE COUNT         1674 a         2125 c         2052 g         2056 t
ORIGIN      
        1 acgggaatgc ggtggtctct ttctaggtat atggcccagg aacggttgaa tctctttata
       61 ttcatccaat ggataccttt gtttcccctt tatgaaagcc atggttactg ggaggaagga
      121 aggagaaaag ggattttgca catcacctgt tcccattgaa tacgctggca tccaactggg
      181 cggttcattg ttaccgttga ttctttggtt tcccacaaga tcgaaaagga aaattttcat
      241 ggatggtaca tcggtagcta ttattgcaag cggggtatta gtgggtatgg gtgcctcctt
      301 taccgggctc ggtggcggat tcttgatggt ccccctgctc ttatttctcg gtttcacggc
      361 acaaaaagcc gttggcacct cattcgtcgg gatcctggtg attgcggtct cggccttgat
      421 tgcccacggg aggatggctc atgtggatta caaatatggc ctcctgctcg gcctgggcgg
      481 aatcatcggg gcacaaatcg gtgcacgcct tgttgagggt gttcccacgg acatgttcaa
      541 gaaaatcttt gcggtcattc tcattggttt ggccatttat ctctttgtga agaaatagga
      601 cccagtcgag accccatccc atcacaaggg gacattggtt cggtacgtcg ttggttttcg
      661 ctaccggata ttcagtgaac ggtaaaatcc atttttgctg atatagctat tttcaataca
      721 tggctcttgc caatacatcc tacttaatag taactatgaa tgggggtggc ttctcttggg
      781 ctgcatgttt gattccagta gccatgactg tccgtcagtg gtgctgagct tccccatgat
      841 agatttttga ggaccgacgc cacagcctca tgtctgtggg ggccggccaa tgtgacgcaa
      901 caaccccgac attccagtat gccattgtgt ttgcggaaag accctaatga ttgagccgat
      961 ttttctccag tccctttttt tactcggaac agccgtagtc gttgtggttg gattccacag
     1021 actccatatc ccttcgccac ttgcctatct gcttgttggc gtgttgcttg gttcatacac
     1081 cccggggccg gtcatcgagg cacaaccggt tcgtgccttg gcagaattcg ggattgtctt
     1141 cttattgttt acgatcggcc tgaatttttc gatgccgcaa atccatgcct tgcgccatca
     1201 gttactgggt ctgggaacgg ggcaggtcct gctcacgacg atcgcggtgg gtctcctggc
     1261 ttggctcgtg gacttaccca ttgccgccgc ctttgtcgtg ggagcggttt tcgcgcagtc
     1321 ctccacgacg attatcagca agcaactcat cgaacaggga gaggagcata gtcggcacgg
     1381 acgattgggg atcgccatgt ccgtttttca ggatgtgaca gccgtcccat tcgtggtcgt
     1441 cattcctgtg ctcgctatgg ctaatacgga ggttgtggtt ctggccggtt cactcgggat
     1501 ggcgtttgcg aaggcagccc tggcgttcgg actggttttt ctggccgggc gatgggtgat
     1561 gcgtccctta ttttatctgg ttgcagagct tcgctccacg gaagtgttca ccctgacagt
     1621 cctgtttgtt tcattggttt cggcatggac tacgagtagt cttggccttt ctatggcttt
     1681 cggtgccttc ctggcaggga tgatgttggg tgagaccgaa ttccgacatc aggtggaatc
     1741 tacgattcgc cctttccgcg atgtgttgct gggtctgttc tttatcggga tcggcatgct
     1801 ggtcgacccg tcgtcaatcc cgggcatctg gcattgggca ttaatgggcg ccacggtctt
     1861 attgctcatc aaggcagctc tggtggcgct gatggtacga ttatcgggta tggatacctt
     1921 gactgcctgg cggaccggat ggctattggc ggtgggaggt gaattcgggt tcgctctgct
     1981 ctccattgca ctggatgtgg gggtgatcca agcaaaaatc ggtcagattg tgttgacctc
     2041 tgtgttattt tctatgattg ttgccccctt tctcattcgg tataaccagg tgcttgcccg
     2101 ctggtgcgca cctcgtccgg cccgggaggg gaaggatgcg gtgccccaag tccgtatcga
     2161 taccctcggg cctcttcggg atcatgtcat catttgcggg tacgggcgca tcggtcagag
     2221 tgtggcccac cttctcgaag aagaaaaaat tccttatgtg gcattggatc tggattcgtc
     2281 cagagtaaag gacgcgaata tcgccggcga gggcgtcttt tacggggatg cttcggaacg
     2341 caccattctt gaaggggttg gtgtcggcac ggcccggttg gtcgtgatcg cccacgccga
     2401 tgtggcctcc gctcataaaa cgctacatca ccttcgcgcc ttacgtccgg atcttccgat
     2461 catggtccgc actcgcgatg aaacccatgt cgatgaattg cgtgcggctg gtgcgacgga
     2521 ggtggtgccg gagacactgg aagcaggtct catgatcgcc gcacatgcct tggtgttgct
     2581 gaatgtgcct atctcccgtg tggcgcgccg tatgcgggaa cagagaaatg ggcggtatca
     2641 tttattacgg gagttttttc tgggtgatag cctgctggcc tacacgaggc aggaacacaa
     2701 cgtcgaccgc cttcgcccgg tggttctgcc gccggacagt tcttttgtgg gacgcacact
     2761 acatgaactg aatctcaatg gcgtcgcgat tgccgctttg gtgcgggaag ggcaacggca
     2821 gctcatgccg tcaggtgaga ccaggctgga ggcatgggat gtcgtggtgt tattcggctc
     2881 gcctgatgat ctacagcgcg ctgaacgcgc cctgctcgaa tgacggggtt atgaatctcg
     2941 cggcgttatc gattgaattc gtcatggaag ccctacgtat ctcgcagtgg atggttgccg
     3001 tgccgtaact ccacgcgttc caaccaattc tgaattgaac gtctcatgag cttcatcgcc
     3061 atgtctttga caaacgtcta cattttcagc atattgctat tggtagttca ttcgacccaa
     3121 ctgatcttta ttgttgtgag gcatttctat ggcaacatgg ctcattccgg cactgaaagc
     3181 cgttcttcca catattggga caattatttc ggccaccgca cccgcattta caaaaaaagg
     3241 aacggccccc accaccgatc agacgcatct gttgcagcaa caaattagcg aattacaaac
     3301 agccgcctct caaaacgcca cacatgtcaa agaactcgcc gaacaattgc aacgcaccgt
     3361 tgcggctatc gaacaggccg cttcagtggc cacatccaat cagcagcgcg ccttgcaact
     3421 cagtcttgcg gcgatcgccc tttccgccat ctccctctgt gccgtgctct tgcatgttct
     3481 cttcctctaa gaccgcattg gtcgcctaag aaaatccgcc atgccactga acggtgatgg
     3541 agttcggagg agtgcgataa tttttgatac cctcatgcgt ttcgacaaca tgtgcgtaca
     3601 gatgcatcat tttatgaatg gaatcggaac actctgaatg agtgggccgg ggcgcgttac
     3661 caggttgagc acctcgcaaa aatcccccaa aagctgtcgt cctgagcaga cacgtgtgga
     3721 tcttgtaggt ttcagccact tgttgcacct ccgaatagca tctgcgcaaa cacttgtatc
     3781 aaggcactac ctccaaacca gaaaacgtcg catatctcat ctgtttaacc aaggaagaac
     3841 gtacccatag cccgatttcc ttttatctat tttggaaatg caggtattaa gagggatagg
     3901 tgttttacag tggttatgga cggcaggttg cacgctgacc cgtggtctgg ccaaagagaa
     3961 ccggtgggga atatgctgcc acgtgaatgg gagcagcctg ggtttgattg tttgaaccta
     4021 catgaagatc gatcggattc aagacaccag aatgagaggc aaacggtaga aggggactgg
     4081 ctaacaaggt tccaggagta ggcgcgatgg tgtcggcctt ggaatcaaca aaagaactaa
     4141 accggccttt ctccccccca catttggtcc catacagagt cgtcaccgga accagatttt
     4201 gcggcaaggg cacaatggta ctactgagga attgtaccgg ggattcaatg ttgaccctgc
     4261 cgctgcctcc gaactgcgaa cgggcatcca agaatgtgaa tgggtccatt acgatcgccg
     4321 atttggccgt gagggtgata tcacctccag atcctgaaac ggcggtactc aaaacctgac
     4381 tatttttgag tacgataaag tctgggtcga aattgatact tccggccgac ccggatcctt
     4441 ccttaacccg gctggtcacc cgactattgg tcaaatcaat gcggttatct gccgtgagtt
     4501 tgatattccc acccaccgcc gaccccgctt ctgtggtcac agaagccttg tcaagcagga
     4561 ttgagtcacc ggccgtgata agaatatccc cggcatttcc cgtgccggtc gtggatgcgc
     4621 taagcgcggc tccatttttc atttcgaaca cgggagaagt aacctgcagg ttccctccta
     4681 atccaggtcc ttccgtgcta ctaaagagtc cacttctcac aaccggtgtt ccctcaagaa
     4741 ttcccactcc ttcggcccgg aaactttccg tcgccatcag ctgaatattg cctcccgccc
     4801 ctgggtcaat ggcaccttcg cgagggatac tgttggctga aatctgcccc ccgctcttta
     4861 tttctatggt tttcgacata acctcaattt gccctgcgtc acccgcccca tcagttctgc
     4921 ttgaaataat actgggcagg ggtcttccga tggcatcagg gaacggggcc atgcccgaca
     4981 tggttaaatg gtcagctgtg agaagaattt ttccgcccgg ccctgcattc tcaggctgaa
     5041 gatttgtcgt accattagtg atttgcgcgc cattagcaat tgtcagattt tttgccgaga
     5101 taataatatc ccctgcctga ttgatcccgt gggttctggt cccaatgctg cttggtctaa
     5161 tcgtccgccc atttgcttca ggaaagggat ccgatcctat aggattttcc ggaaattccg
     5221 tacggagaag gtaaccggaa aggaataaat tctcatgtgc cgtaatggtc acgttccccc
     5281 gctgaacccc cggagtaccg gattgagatt cagcggtgac atcggctcca tcacggataa
     5341 caatcgtatt ggcggtcagc gaaatatttc cggttcctgc cggtccgata actccagcgc
     5401 gaatggtggt tggagcccaa ccaaaaactc cagatccccc aaccgttcgc gcccctgata
     5461 ggattattgt gtcagcgtta acagtgatat ttcctgcacg cgggcgctgt tcctttggca
     5521 tgttggcaac gattttctct atggcatccg ttataggtat cactcttccg gaggatgacg
     5581 gggctatgat ttccgcccca tctttcatca cgacatgttt tgctgtaatc gaaatatcgc
     5641 cggcattacc aagaggtaaa tccgctagcc gattgtcgtg ttcctctgcg cgcgcaataa
     5701 ttcgacttga tatttcttga gtcttgctgg tgttattgat ttctggatca tacacgctca
     5761 tgaatccggt gagagaaaca ttttcttcgg cagtgatact cacccggccg gcatttccac
     5821 ttccccggcc tcttgttctt gatgacatcc atgcaccatc cagcatttca acatttttgg
     5881 ccgtgatggt aatgtcacca ccatgaccgg gaccctgtgt cccactagaa atctcagttc
     5941 taaaaattgt cctgttcttg tccacttttt cgtacgcacc tgacaatcga atcgtatcca
     6001 caacgtcaag gtgtatgctt cccccagctc ccgctccatc taaagcagca ttcgtgatta
     6061 agcctccgcc ggtaacagat acattttctg cactgatctg aatatcgcct ccgcgattca
     6121 cgcccttggt gtgagcgcga agtgcactcg gaacgacggt tcgggggtta agtgtgtttt
     6181 gtggaagaag attcggtgga aactccgttc gaatcgcggt tcccgagatg acaagatcct
     6241 cttctgcatg tatccggata ctgccggttt tttctatttt ggttatggat tccgtttctg
     6301 aggtgaccac acctccatct tcaatcctga tattgtctgc ttccaacaga atgtgccccg
     6361 ccgaagccgg gccagtcgcg ctggctgtca tctcacttgg aataatgtca atgacattcc
     6421 catccatatc ggctccagta agttccagcg tggccgcctt aatgttcacg gtcccggcag
     6481 atggagcatt atcgagcatg tgcatgtcag gttgctcctg tcctattgag gcgacatgta
     6541 tttttgcccc atccaggatc cggacagttt gaccggtaac attgatcgtt ccgccggatc
     6601 ccagaattcc attccccgga aaagtcaatt cactggcacc cgcaaagagg cccgatgaag
     6661 cgatgccatt ggcatgtgtg cctgaaagaa gaacgtctcc ggtagccgta ataaagattt
     6721 ttccggcatc gccgctgcct cgtccaacgg tgccggccga aatgcgagcc ccttcactta
     6781 tggtgagatt tccggtctgc acggtgatgg tacccgcctg acctgttccc accgtgttgg
     6841 caaacaagcc gctcacggcc tcttcccctg gcgaaaaatc agtcaagcga ttatcactcc
     6901 cggataggat gacctcgcca tcaggggcag tgaccataat ggttcccgca tcacctgccc
     6961 ctcgggtggt cactgacatg tgtccgccat cggtcaactc caccgtttgt gcactgatcg
     7021 taatcgttcc ccccttcccg gagccttctg tcctggcaaa tatcccgctt ggagtgtccg
     7081 tttgcacgcc ggttcccgct accaggacat tttcgcttac ttcaatatcg acatggccac
     7141 cgtcctgccc ccccagcgta ttggcacgaa tttgggagtc atgatcgagg accaatcggc
     7201 cgccccgaat caacaccgtg ccaccatgtt ctccgccggc atccatgccg gactggtccg
     7261 agatccggat ggtcccaccc gactctatgg tttgcctatg aatattagaa gtcttgtcca
     7321 aagtatgcgc ttgaatttct cccggcgagc cgacactagc cagggcaatc tgccctccgg
     7381 gtgcagtgag attgctacgg ccaatggtga tgtcgccgcc tacgagcgaa agcgtttggc
     7441 cttccgatac cgacaacgta ctgccttcaa ccgaaatgcc ggctggattc gggccgagaa
     7501 atccaaaggc cgcaatcgga gcaatactga gcagggaatc ctgtgcaccg ggcagggcgg
     7561 tgaactgggc cccatctgca agccgcacat aatcagcggt cgtaaaatgg gtggccccgc
     7621 ccacattcaa ggaagcattg gggccgaaga caattcccgc gggattcatc aaaaagaaat
     7681 tggcattgcc gaagccttcg gtctgaatgg ttccaaaaat attggaagga ttgccaccgg
     7741 tgacgcgact gaggatgttt gaagtcggaa gtcccgtatc gttcaggaag ttggcaatat
     7801 tgtccgtggg aacaccaaaa tccccaaagc tatggaagag attgcccccg ccacccggtc
     7861 tcgttccacc ggtaatatta tgttgaaccg ctccgccggg aagagac
//
LOCUS       DATIAL010000343        14743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_708306, whole genome shotgun sequence.
ACCESSION   DATIAL010000343 DATIAL010000000
VERSION     DATIAL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708306"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VLA60_10650"
     CDS             complement(<1..522)
                     /locus_tag="VLA60_10650"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HSF09866.1"
                     /translation="MGIFREYDIRGIVAYDLGPDVVERIGRAYATLARARGVHNVAVG
                     RDGRLTSPALRDHLVAGLTSSGVNVLDLGLCATPLLYFSLFSCDVDGGIMITGSHNAS
                     EYNGFKMCIGREALYGADIQELQVIYESGEFASGSGTVLPQSIIPEYLKFLQEHFSLL
                     RAQGLRVVIDCGNG"
     gene            complement(546..1994)
                     /locus_tag="VLA60_10655"
     CDS             complement(546..1994)
                     /locus_tag="VLA60_10655"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HSF09867.1"
                     /translation="MNPHVYGVILAGGSGTRFWPLSRERFPKQLLRILGEGTLLQQTF
                     ERLLQNIPANRMAIVTNAVQAESIKLQLNQWKDDIAGNVILEPEGRNTAPAIALAALQ
                     LMHQDPEAVMVVVPADHVVKASKKFMRAVKFASELAMGGHLVTFGIQPTRPETGYGYI
                     QPLKRMRVGAKGPFIGYSVARFVEKPNLTTAKRYFRSGNYFWNSGIFVWKASQILSEL
                     AVHQPALSKLLTGLQGKMGREDFPSHLRKAYAKVESLSIDNAVMEHSSRSVVIPIDFG
                     WSDVGSWSSLEEVVPLDKDGNVRNGNIIDLGSRDSVLFADRRVVATIGLDNMVVVDTP
                     DATLVCPKDRAQDVKAIVNLLKRQGAPEHLEHRTVHRPWGSYTVMEEGKGYKVKRIEV
                     APGKRLSLQLHHQRSEHWVVIAGTARVTRGEEVYDLQSGMSTGIAKETPHRLENPGQI
                     PLEIIEIQNGPYLGEDDIVRLQDDFGRLRPIG"
     gene            complement(2134..2721)
                     /locus_tag="VLA60_10660"
     CDS             complement(2134..2721)
                     /locus_tag="VLA60_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HSF09868.1"
                     /translation="MRKNQGIASLSKLKGAKTVRPLKKKDSPRSLNRPEKVFKELRLF
                     ATDVDGVLTDAGMYYGESGEELKKFHTRDGMGIKLLQAQGMITAIITMENTKIVARRG
                     KKLGIPEVFQGAKDKVAILRHLSEKYGIPFSQMAYIGDDVNDVEALKTVGYAAAPADC
                     VEQVRQVVHYVCKKNGGEGAVREVIDRILAAKSPQ"
     gene            complement(4109..4900)
                     /locus_tag="VLA60_10665"
     CDS             complement(4109..4900)
                     /locus_tag="VLA60_10665"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HSF09869.1"
                     /translation="MLSLKKILASFFSPLSLCIEVMACGLLFLCFSRKQYLGKILVSL
                     GFILLVISGYEGVSGRIIRTLESQYPPINLSQVLTSEGTANTQESVKWIVVLASGTTG
                     DATLPYQLRVSHHSRVRLMEGIRLHRLLPGSKIILTGGTGFEGSPEATTMSRVAEELG
                     VSRADMVLEVESRDTKDHPLYVRDIIHDEPFILVTSAFHMPRAVKLFEKQGLFPIPAS
                     TGQWIPPMQFWSLVNFFPSSSGVRLAELAYHEYMGLLEAWVQGQI"
     gene            complement(5267..5644)
                     /locus_tag="VLA60_10670"
     CDS             complement(5267..5644)
                     /locus_tag="VLA60_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09870.1"
                     /translation="MSMRFWSLIVWFLLVLPPSAFSEVWDFSCTEAVSLLRAAQEQVV
                     RKHDQLQEAKFSLRHAPKDFDGCRRSRRGFQGGEIHCVTHQSPQGNLLRDILVAQRSL
                     DVSIQNFKKHQKGLVLSCSEPSP"
     gene            complement(5821..6429)
                     /locus_tag="VLA60_10675"
     CDS             complement(5821..6429)
                     /locus_tag="VLA60_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF09871.1"
                     /translation="MRKLFQGKYAILGLLVAILSGCSSWHHVSIFSHGAEPVEESPDS
                     SPASVVSAPPVSVPEPEPSLPIVSQPPISEIPPAIDISAQSADHSLLPSTLEDVFFDY
                     DQYYIRSEAVPILMQNAEVLLNRHATRTVLIEGHCDERGTEEYNLILGERRAMAVKNY
                     LVDLGVSASSIRVLSLGKNEPFCLQPTRECFQQNRRAHFVLK"
     gene            6674..7639
                     /locus_tag="VLA60_10680"
     CDS             6674..7639
                     /locus_tag="VLA60_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF09872.1"
                     /translation="MIDVQHVTKRYGNATALDDVSFSINKGEIVAFLGPNGAGKTTTM
                     RILTGFMPPTLGTVRIAGFDCLETPWEVKKHIGYLPETPPLYLELTVTEYLTFVGRIK
                     RLSEDQLIERMGTIITQTGLADVRGRVIGHLSRGYRQRVGLAQALLHNPPVLILDEPT
                     TGLDPNQIIEIRELIKSLAGSHTIILSTHLLAEATAICQRVIIIHRGRIVAVDTPEQL
                     GAKLRESETLSLTVKQFDPDLLEALRHIPGVLRVSQGATAHTYLLDTQMGRDIREELT
                     QLVVSRNVGLLELKTQPLTLEDAFKVLTQTNTPSGPGISTPDGPH"
     gene            7667..8437
                     /locus_tag="VLA60_10685"
     CDS             7667..8437
                     /locus_tag="VLA60_10685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF09873.1"
                     /translation="MTPVHTIVAKELRSAFVSPVVYVIAAIFLAIVGLLAYSAAANAS
                     NQAIRLMQIQNTYAQLNLNDMLFRPLFRSINLILMIILPLLTMRLFAEERKLRTFELL
                     LTSPIGVNEIVSGKFMSVIIVYSGLLSLTSLIPITLSAYATFDWRPIYLGYLALFLQG
                     ALLISVGLFASALTENQIIAAFLSFGCILGLWMLGALGGIVGDTTIGHILSYLSFSEH
                     YERLIRGLFNLKDILYYVSGIAFMWFAAHQAVDAYRWK"
     gene            8443..9951
                     /locus_tag="VLA60_10690"
     CDS             8443..9951
                     /locus_tag="VLA60_10690"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HSF09874.1"
                     /translation="MPSILGIAGTIIALVGLGLPLVYPAGESFSSILLGLGTLCLLGY
                     FAFHFRSITALARTRSTRLGAHSLFSILLAGVVVTLLNFLAAKHAPEWDFSETQNFTL
                     SRQTYQVLRTLPREVKMTVFTHEGSPGYGGYQDLLTTYAKESPLITLTFIDPERQPDK
                     AKEYQVTRIDTVVVESGPQKVYLQRSSEADLTNALLRVTQDKKKRLAFVTGHGEKSLS
                     DTESSGLSRAGDALTKQGYEVGTVDWQDTASHEAAVLIVASSTEAVSSDDQQRISQFL
                     EKGGRLLVMDDPGSGTSLDPLFTPWGIHVGTGILADEQDRLGRGSPTALLVRTFTTHD
                     ITEDFTTPILFPVSRSVTFDTAKGKAWEYVALAQTSPESWEETDLNSPQPEFTAGQDP
                     KGPFTVAGLLIRKPVDQSPTDQSAVLIVGNAAFATNGYLTYPGNTDFFLKAIAWLAQE
                     DALVSLTPKDPAFHPFIPNPSQEQALVFFQVLFIPLLTLFIGLSVWKRRRSL"
     gene            9953..11326
                     /locus_tag="VLA60_10695"
     CDS             9953..11326
                     /locus_tag="VLA60_10695"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HSF09875.1"
                     /translation="MINPVRVTLILALVVLGLGGYILLVDIPQSRQLEKQETQERQIL
                     PFDDRAITQIIWATPTDTIRLERDDQWRWMMTEPMRSPADSREIRRILRALTIGKIKR
                     HIEDGPSNLSAYGLEPPYLTLTLTTPTETQEMALGDAGPFAPSLYVQTKPDNQVVLTT
                     LDVMTFAQKSLTNFRLKDLLFFERDRVLELHIQLGSTTMVMTRVAGAHSLTPNWMFQS
                     PVKGPADKTAVGTLLMDLGGLAAIGFIDTEDQKKRILGQPARIQATIQVVEGARTHHL
                     ELYQFADPEKAYAITSASGPLYEIPPGILKPITQGMFHFQDKRLFGMEVADIAMLTVH
                     TPQDHYVLINQHDEWVLEDNPSAEVDQEVVKLFVSRIVDIPAEIFHPDSSSASTDHGM
                     ASPNATISGMNRKGQEIGQLILGKRERGLVFAKGASLPGLYQVRSTILDQIPKKIQLI
                     RQAGSTE"
     gene            complement(11452..11646)
                     /locus_tag="VLA60_10700"
     CDS             complement(11452..11646)
                     /locus_tag="VLA60_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09876.1"
                     /translation="MNGSNATTQVTVIICSIFLMGAMMVGCSSTPEPQSDPSKKDIQQ
                     DSDRFFKKMGQEEGQQAPAR"
     gene            11770..11970
                     /locus_tag="VLA60_10705"
     CDS             11770..11970
                     /locus_tag="VLA60_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09877.1"
                     /translation="MSGTVLAIFKCHDHTLDTLLGAMAGEGIRLTKMGIDQIPHSGKP
                     DQLLDHYEIRARAIVDKVKQLV"
     gene            11983..12678
                     /locus_tag="VLA60_10710"
     CDS             11983..12678
                     /locus_tag="VLA60_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSF09878.1"
                     /translation="MPPPSNFDLIPLFACLSESDRRELANHAIEVPITKGQFIFREGD
                     PADWFHIVKQGNVKCVKSSPEGREVTLKVLMPGDLFCCEASAFSGEGHPGCAQSMGEG
                     TIVKIHRKVMLDVIQRNPGAAVNIINYLSLRLRESQDNAKAFALDRAEQRVASILVNL
                     AQRSGIQEATGIRLAVRLTHQDLADMAGLTKETTSRILSRFKKDHMISGTGKKLVIQD
                     LTRLQQMAPHAFS"
     gene            12875..13918
                     /gene="nirK"
                     /locus_tag="VLA60_10715"
     CDS             12875..13918
                     /gene="nirK"
                     /locus_tag="VLA60_10715"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HSF09879.1"
                     /translation="MTHLAFTHSIRSTGFISLATMLGVLAMGSVSPEPAMSMPTIQAQ
                     LTTLPKVPTPVTRKEAAHVVVNVEAKEYVGTLADGVQYKFWSFNGTVPGPMIRVREGD
                     TVEVHLKNHPESVFPHNMDLHAVNGPGGGAGSNLAIPGKEGMFAFKALNPGLYIYHCA
                     SPVPNIPAHIANGMYGLILVDPAEGLPPVDKEFAVFQSEFFTMPSAEKGIMELSMEKG
                     LAEHPDHVVLNGRAGALIGDGQLEAKAGDTIRLYVGNIGPNSTSSFHIIGEIFDKVYV
                     EGAIGGTINKNVQTTLIPAAGATIVEFKVDVPGAYLLLDHSIFRVAKGAVGILSITGE
                     ENPSIYNSMDPAN"
     gene            complement(14021..14341)
                     /locus_tag="VLA60_10720"
     CDS             complement(14021..14341)
                     /locus_tag="VLA60_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF09880.1"
                     /translation="MNVVNIKDYGQFSKEKMKKQNVFQSSRFFCDVYGFEPGQEQKGH
                     VHADQDKVYLVLEGEGTFRVGSESQVLGEGQGTMAPAGEDHGVVNHTTNRLRVLVFMA
                     PNPG"
BASE COUNT         3823 a         3998 c         3331 g         3591 t
ORIGIN      
        1 cccatttccg caatcaatca caacccgaag tccctgggct ctcagcaaag aaaaatgttc
       61 ttgtaaaaat ttgagatatt caggaatgat ggattggggt aagaccgttc ctgatccgga
      121 tgcaaactcg ccgctttcat aaattacctg taattcttga atatcagccc cgtacaatgc
      181 ttcccggcct atacacatct taaatccgtt atactcagat gcattgtgac tgccggtaat
      241 cataataccc ccgtcaacat cacaggaaaa caaagaaaaa tacagtaagg gggtcgcaca
      301 tagaccaaga tcaagtacat tgactcctga actggtcagg ccggcaacaa gatgatcccg
      361 aagtgctggt gaagttaaac ggccatcccg gcccaccgcg acattgtgga caccgcgtgc
      421 tctcgccagg gtcgcatagg cacggccgat gcgttcaacc acatcaggac ctaaatcgta
      481 tgcaacaatc ccgcgaatat catattctcg aaaaattccc atccggctct ctttccttcc
      541 gaatcctacc caatgggtcg taaccgacca aaatcgtcct gaagtcgaac gatatcatct
      601 tctccaaggt agggcccatt ttgaatttca ataatctcta aagggatctg cccgggattt
      661 tctagacgat gcggagtttc cttggctatc cccgtactca ttcccgactg caagtcgtaa
      721 acttcttcgc cacgcgtcac ccgggccgtc cctgcaatga ccacccaatg ctcactgcgt
      781 tgatgatgga gttgaaggga aagccgtttt ccaggggcga cttcaatccg tttaacttta
      841 tatcctttcc cctcttccat gacggtatag gaaccccaag gacgatgcac ggttcgatgc
      901 tctaaatgtt caggggcgcc ctgtcgcttc aacagattaa caatggcttt gacatcttgc
      961 gcacgatctt tcggacagac cagggtggca tcaggcgtat ccaccaccac catattgtcg
     1021 aggccaatcg tggccacgac ccttcgatcc gcaaacagaa ccgaatcgcg gcttcccaga
     1081 tctataatat tcccattccg gacatttcca tctttatcca aaggcaccac ttcctccaag
     1141 ctgctccaac tccccacatc cgaccaacca aagtctatcg gtatcaccac gcttcgagaa
     1201 gaatgttcca tcaccgcatt gtcaatagaa agcgattcga ctttggcata cgccttacga
     1261 aggtgggagg gaaaatcctc acgtcccatc ttgccttgta aaccagtcaa caatttggat
     1321 aacgccggtt gatgaacggc taattctgat aatatctggg aagctttcca gacaaaaata
     1381 ccgctattcc aaaaataatt tcccgatcga aaatatcgtt tggcagttgt gaggttcggc
     1441 ttttcaacaa agcgggccac cgaatacccg atgaaggggc ccttggctcc gaccctcatt
     1501 cgcttcaaag gttgaatata gccataccct gtttcgggac gagtcggctg aatcccaaac
     1561 gtcacaagat gcccacccat tgccaattca ctcgcaaatt ttacggcccg cataaatttt
     1621 ttggaggcct tcacgacatg atcggccggc accaccacca tcacggcttc aggatcttga
     1681 tgcatcaatt gtagggccgc gagagcgatc gccggtgcgg tatttcttcc ttccggctca
     1741 agaatgacat ttccagcaat gtcatctttc cactgattga gttggagttt gatggattcc
     1801 gcttgaacgg cattggtgac aatcgccatc cgattcgcgg ggatattctg taatagacgc
     1861 tcaaacgtct gctgcaggag ggtgccttct cccaaaattc tcaacaattg tttaggaaat
     1921 cgttcccggc tcaacggcca aaaccgggta ccactccccc cggctaaaat cactccatag
     1981 acatgagggt tcatgttcga tccttctcct ccaagtgttt caaacggtca aagggaccga
     2041 atggaagacc atactcaaat gactaacggt taaaatctca taagaaagat tgtcggaatg
     2101 tgccattcaa aaatccactc aataccagta gggctattga gggctttttg ccgccaaaat
     2161 tctatcgata acttctcgaa cagctccctc acccccattt tttttgcaga cataatgcac
     2221 aacctgacgg acttgctcta cacaatccgc gggagccgcg gcgtatccca cagttttcaa
     2281 ggcctccacg tcattcacat catccccaat gtaggccatt tgtgaaaaag gaatcccgta
     2341 cttttcagac aaatggcgga gaattgccac cttatccttg gcaccttgaa agacttcggg
     2401 aatgcctaat ttttttccac ggcgagcgac aattttggtg ttttccatgg taatgatggc
     2461 cgtgatcatc ccttgggcct gtaagagttt aatccccata ccatcacgcg tgtgaaattt
     2521 tttcaattct tcgcccgatt ccccatagta catgccggca tcggtcagaa ccccatccac
     2581 atccgttgca aataatctca gctctttgaa gactttctct ggtctattta acgaccgtgg
     2641 agaatctttt tttttgagag gacgtactgt ttttgcccct ttcaatttgg ataaagaagc
     2701 gattccttgg ttttttctca cctgttcatt tctacctttc tatgtcacat ttcataggca
     2761 atcctacaga tacgcttaag tttaaggcag atcttccgtt aagaccacag gagggataga
     2821 atccgtaggc aggcaaattt aacaatcaat cgtccgtttt ttaattattt tcggaatcca
     2881 gttttgcgaa accggctcag acacggctta cttgtgagtt cttattttcg attcctcatg
     2941 atcttagctc tttgttccag tcttctttcc tgttcatccc cccaatctca gcaaagactg
     3001 gacaccgtat tatgttggct acgcccatct tgtcttccct cagaactccc atccctcagc
     3061 gaaaacgtac ctcaagccga tacaccccca gagcccatta acgcaatcgt ttcttcagag
     3121 gagtctcaac ccctatctcc gcaggtttcg tcttctgaaa taagagatgc ctcgaattgt
     3181 ctcacgtctg ctcaaatcgc cattacggcg tcaaatcctg ttgtcagcat ttcgtatatt
     3241 gaacccacaa caaaagccaa cggccaggca ctaacgaatc ttgccaagac taccatttac
     3301 caagatatag gaaaaggatt actcaaatat aaagagatca aagcgaccaa tcccggagga
     3361 gggggaacca ttcgagaaaa agtttcgttc agccttaagc cggaggaaac ccttcaggcc
     3421 acaatttgca taaccgcgac cgatacaaat ggccaggaga gataagcttg cataggatga
     3481 acctcaacgg tcttaagctt cattcaacct ggaggagcca tgagggtggt gtgcagatga
     3541 gtagtgggag gtcatttgct taaaatgaca tcgtaaagtt ttctcgtttt gtcacggctt
     3601 gaggataaat ccttgttgtt ccatacactc ccttatttcc aatccctcgg taaccgaatc
     3661 tctcttccaa tcatttccct gttccgcaga aaccagcgca acacaggctg aatgggtgtc
     3721 atttggatcc gtacctcctc catcagtgga aacccattcc ccttgtaggc agtccccata
     3781 gggatggcaa atacgatccg cacgagtaga gccatggttc ggatgataat acgattccca
     3841 cagactacac ccccccagga agaaggcact taggcctatt atgagggccc ctcccgccca
     3901 tctttgccaa ttcattactt tgctcccaaa aatcccagcc atattgtgaa ccaaacaccc
     3961 agattacatt cttttgtatc tctatcggac agcctgagct gagaaattag gccattcata
     4021 ccccgaatca gcgccgggac attaactctc tcaattgatg tttgattcgc cttaaacggc
     4081 caagattcag cgcctcgctt ctgcttcttt aaatctggcc ttgaacccag gcctccaata
     4141 accccatata ttcatggtag gccaattcgg caaggcgcac tccagaggaa ctcgggaaaa
     4201 agttcactaa agaccaaaac tgcatcggag gaatccattg acctgtggag gccggaattg
     4261 gaaatagacc ctgtttctca aataatttga ccgcacgtgg catgtggaaa gcacttgtca
     4321 ccaggataaa aggctcatca tggatgatat cacgaacata aagaggatga tccttggtgt
     4381 cacgggattc aacttccaga accatatctg cccgactgac gcctaattcc tcggcgactc
     4441 gactcattgt ggtcgcttca ggggacccct caaaccccgt cccccctgtc agaatgattt
     4501 tacttccggg cagcaggcga tgaagccgaa tgccctccat taatcgaacg cgggaatgat
     4561 gagacactcg aagttgatac ggtaaggtgg catctccggt agtaccgctg gccaggacca
     4621 caatccattt cacggattct tgggtatttg cggtaccttc agaagtcaga acctgggaaa
     4681 gattaatggg gggatattgt gattccaagg tacgtatgat acgccccgag acaccttcat
     4741 aaccgctgat gacaagcaga atgaatccca gggaaaccag gattttccca aggtattgct
     4801 ttcgggaaaa acacaaaaag agaagcccgc aagccatcac ctctatgcac agagagagag
     4861 gagaaaaaaa tgaggctaaa atctttttaa gactgagcac caaatggtct tttcctccta
     4921 tcgatgatga tcctagggaa agctccgccg attcatttcg cggcacagat cgatgattat
     4981 acgaattttt ccattttttt tcttcttggt tgtcaaaagg tggattgggc taaagccggc
     5041 atcattcgct tcgagcagga tgaccaaaac aaccatgatc gctccacggg atgggtgcgc
     5101 gaagcgataa taaaaggcac ccaccttgga gtggggggat gaaccattga ctatcctgga
     5161 ttttaacggg ggcaaaccct tggcaggagg atcgatgaag caccgccctg agaggggata
     5221 actatcgcgc gggatggaat acgaggtaag aaaaaagaat atcggattag ggagaaggct
     5281 ccgagcagga aaggaccagt cctttttggt gttttttgaa attctggata gagacatcaa
     5341 ggcttcgttg cgcaacaagg atatctctca ataggttgcc ttggggagat tgatgggtca
     5401 cgcaatgaat ctctcccccc tgaaatccac gacggctccg cctgcatcca tcaaaatcct
     5461 tgggagcatg ccgtagacta aattttgctt cttgcaattg gtcatgcttc cggacgacct
     5521 gttcctgagc ggctcttaac agagagacag cctcggtaca ggaaaaatcc caaacctccg
     5581 agaaagccga cgggggaagc accagtaaga accagacaat taaggaccaa aaccgcatag
     5641 acatcctcct catcctgagg aaaagggcaa aaatattgtt gagagacttg caaattttct
     5701 cccaaaatat cccgcgattt tgaccccttc aataacctaa aaggagggac agccatgatg
     5761 gaccggattc atatgagttc taaagacgta atcacacata ttggggaaga tttaaccgaa
     5821 ttacttcaac acaaagtggg cccgtctatt ttgttgaaaa cattcacgag tgggttgaag
     5881 acaaaacggt tcatttttcc ctaagctaag gactcggatt gaggaagcgc tcacgcctaa
     5941 atccaccaga taattcttca ccgccatcgc gcgacgctca cccaaaatga gattatattc
     6001 ctcggttcct cgctcatcac aatgcccttc gatcaggaca gtacgagtag cgtggcgatt
     6061 caggagtact tcagcgttct gcatcaggat aggaaccgcc tcactccgta tataatattg
     6121 gtcgtagtca aaaaaaacat cttccaacgt cgaggggaga agggagtgat cggcagattg
     6181 ggcactgatg tctatagccg gcggtatttc tgatattgga ggttgggaaa cgattggaag
     6241 agagggttcc ggttctggta cagaaacggg aggggcactc accaccgaag caggagatga
     6301 atcgggagac tcctcgaccg gctcggcacc atggctgaag atggacacat gatgccagga
     6361 actgcacccg ctcagaatag caaccaacag tccaaggatc gcatattttc cctgaaacaa
     6421 cttcctcaat ggtggccgtt actccactct cccaaggcag ggtagaagtt cccttgctcg
     6481 aatagctcaa aaatccctaa acgtgtaatt atcatacaac tttcatcaaa agagtcaatc
     6541 tcagtcggca ctgccctcat gtttgaactt gacggatttt ttttcacata ggtatgatcg
     6601 gactcctgtc gagccattgc attttaaagt cacttttctc accatttcac gacacaactt
     6661 gctgggcatc cgtatgattg acgttcaaca tgtgaccaaa cgctacggga acgcaaccgc
     6721 gttggatgac gtctctttct ccattaacaa gggggaaatc gtggcatttc ttgggccaaa
     6781 tggcgcagga aaaaccacga caatgcgcat tctgacaggg ttcatgcccc caactctggg
     6841 gaccgtccgc attgcagggt ttgattgtct ggaaacaccc tgggaggtca aaaaacatat
     6901 cgggtatctc cccgaaactc ctccacttta tctggaactg accgtcactg aatatttaac
     6961 ctttgtggga agaattaaac gactttctga ggaccagttg atcgaacgaa tgggtaccat
     7021 catcacccaa acgggcctgg ccgatgttcg gggacgcgtc attggacatt tatcccgtgg
     7081 atatcgacaa cgggttggcc tggcacaagc tcttttgcac aatcctcccg tgttgatttt
     7141 ggacgagcca accacagggc tagacccgaa tcaaattatt gagatccggg aactcattaa
     7201 aagcctggcg ggctcccaca ccatcattct cagtacacat ctactggccg aggcaaccgc
     7261 tatttgtcag cgggtcatta tcattcaccg tggccgaatt gtagcggtgg acaccccgga
     7321 acagttaggg gccaaactcc gtgaatcgga aacgctcagt ctaaccgtga agcaattcga
     7381 tccggatctg ttagaggcac tgcgccatat ccccggagtc ctccgagtct ctcaaggagc
     7441 cacggctcac acctatcttc tggatacgca gatggggcgg gatatccggg aggaattgac
     7501 tcaattagtc gtctccagaa atgtgggcct tctggaatta aaaacccaac ctctcactct
     7561 tgaggatgcg tttaaggtac tcacacaaac caacacgccc agcggacccg gcatcagcac
     7621 gcctgatggg ccacactaac cgccctatac ccggattact ctcattatga cccccgttca
     7681 tacgattgtt gccaaagaat tacgaagtgc gtttgtgtct cctgtggtct atgtcattgc
     7741 ggccattttt ttggccattg tgggcctgtt ggcctattcc gcggcggcca atgcgagtaa
     7801 ccaggctatc cgcttgatgc aaatacaaaa cacctacgca caactcaatc ttaatgatat
     7861 gttgttcagg ccactttttc gaagcattaa cctcatcctc atgattattc tcccattact
     7921 caccatgcgt ctcttcgccg aagaacgtaa actccgaacc ttcgaactcc tgctcacatc
     7981 tcccattggc gtgaatgaaa tcgtgtctgg aaaattcatg agcgtgatta tcgtctatag
     8041 cggcctgtta tcactaacaa gcctcatccc gatcacgctt tccgcctatg caacctttga
     8101 ttggcgacca atataccttg ggtatctcgc cctgttcctg caaggagccc ttctgatatc
     8161 cgttggttta tttgcctcgg cattgaccga aaatcaaatt attgcggctt ttttaagctt
     8221 tggctgcatc ttaggtctct ggatgctcgg ggcgttagga ggcattgttg gggacaccac
     8281 gatcggacac attctgtcct atctctcttt tagcgaacat tatgaacgat tgattcgtgg
     8341 gctcttcaat ctcaaagata tcctttatta cgtctccggc atagctttta tgtggtttgc
     8401 cgctcaccaa gccgtcgacg cctaccgatg gaagtaacgc gtgtgccttc aattctcgga
     8461 atagccggaa cgataatagc tctggtagga ctggggctcc ccttggtcta tcctgctgga
     8521 gagagcttct cctcaatcct gcttggcctg ggaacactct gcctcctggg ctattttgcc
     8581 ttccattttc ggagcatcac agcgttagct cgaacccggt ctacccgact cggcgcacac
     8641 agcctgtttt cgatcctgct tgccggcgtc gtcgtgacct tactcaattt cctcgcagcc
     8701 aaacatgcgc cggaatggga cttctcagag acacaaaatt tcaccttaag ccgccagacc
     8761 tatcaagtcc ttcggactct tccaagagaa gtgaaaatga cggtcttcac ccatgaaggg
     8821 agtccgggtt atggcggcta ccaggaccta ttgacgacct atgcgaagga gagtcctctc
     8881 atcacactga cattcatcga tcccgaacgc caacccgaca aggcgaaaga gtatcaagtg
     8941 actcgaatcg ataccgtcgt cgtggaaagt ggtccccaaa aggtctatct ccaaaggtct
     9001 tctgaagccg acctgaccaa tgcgctgctc cgcgtcacac aggacaagaa aaagcgcctt
     9061 gcctttgtca cgggccatgg tgaaaaaagt ctctccgata ccgaatcgtc aggattatct
     9121 cgtgccggcg atgccctgac caagcaaggc tatgaggtcg ggacagtgga ttggcaagac
     9181 acggcttctc acgaagcggc agtcctcatc gtggcctcct ccacagaagc cgtatcctct
     9241 gatgaccaac aacggataag tcaatttctg gaaaaaggag gacggctact ggtcatggat
     9301 gatccaggca gcggcacgtc tttagatccc ctcttcacgc cgtggggcat tcatgtggga
     9361 accggcatcc tggctgatga acaagaccgc ttgggacgtg gcagtcccac cgccttgctc
     9421 gttcgaacct ttaccacaca tgacatcacc gaagacttca ccactcctat cctgtttccc
     9481 gtatcccgct cagtgacctt cgacacggca aaaggaaagg cctgggagta tgtcgcctta
     9541 gcacaaacct ctccggaaag ttgggaagaa acagacctga acagtcctca accggaattc
     9601 actgcggggc aagaccccaa aggccctttt accgtcgcgg gcctcctgat ccgcaaaccg
     9661 gtcgaccagt ccccaacaga tcaatctgcc gtcctcatcg tgggcaatgc cgcgtttgcg
     9721 accaatgggt atctgaccta tccgggaaac acggattttt tcttaaaagc cattgcctgg
     9781 cttgctcagg aggacgcctt agtttctctg acaccaaaag atccggcctt tcatccattc
     9841 ataccgaatc cctcacagga acaagcgctc gtattttttc aggtcctgtt cattccactc
     9901 ctcaccttat ttatcggtct atctgtttgg aaaaggcggc gaagtctata acatgataaa
     9961 tcccgttcga gtgacgctca tcctggctct ggtcgttctg ggtttgggag gctatattct
    10021 cctcgttgat attccacaat cccggcaact ggaaaaacag gagacccagg aacgacagat
    10081 cctgccgttt gatgaccggg ctatcacgca gatcatctgg gccacaccca cggacaccat
    10141 ccgtctcgaa cgtgatgacc aatggcgatg gatgatgacc gaaccgatgc gatcccctgc
    10201 ggattcgcgt gaaattcggc ggatcctgcg agccttgacg ataggaaaaa tcaaacgaca
    10261 tatcgaggat ggtccaagca atttgtccgc atacggatta gagccccctt atctcaccct
    10321 caccctgacc actccaaccg agacgcagga aatggctcta ggggatgccg ggccctttgc
    10381 tccgtcctta tacgtccaaa cgaaacctga taatcaggtc gtgctgacaa ctttggatgt
    10441 gatgaccttc gcccaaaagt ccctcacgaa ctttcggctc aaagacctct tgtttttcga
    10501 acgggatcga gtcttggagc ttcacattca gctcggatca actaccatgg tcatgacacg
    10561 agtggcagga gcccatagct tgacgccgaa ttggatgttc caaagtcccg tgaagggacc
    10621 ggcagacaaa accgccgtgg gcacactcct catggattta ggaggcttgg ccgccattgg
    10681 attcattgac accgaggacc aaaagaaacg gattctgggc caaccggcgc gcattcaggc
    10741 gacgatacaa gtggtagaag gcgcacgcac gcatcatctg gaactctatc aatttgctga
    10801 tccggaaaag gcctatgcca tcacgtccgc ctccggtcca ctctatgaga tcccaccggg
    10861 catcctgaaa cccatcacgc aaggaatgtt tcattttcaa gacaagcgtc tctttggcat
    10921 ggaagtcgcc gatatcgcga tgctgaccgt ccacacgcca caagatcatt atgtcctcat
    10981 taatcaacac gatgagtggg tcttggagga caatccttca gcagaagtag accaggaagt
    11041 tgtgaaactt tttgtcagcc ggatcgtaga cataccagca gaaatattcc accctgattc
    11101 ctcttccgcc tcaacagacc atgggatggc ctcacccaac gccacgattt ctggcatgaa
    11161 ccgcaaggga caggaaatcg gacaattaat tttgggaaag agggagcggg gactggtctt
    11221 cgccaagggc gcaagtctgc caggtttata ccaggtccga tcgacgattc ttgatcaaat
    11281 acccaaaaaa atccaactga tacggcaggc gggttccacc gaatagactt cctgctcact
    11341 ccttacatcg ccgtttcagg cctcaaatgg cataattgcg ccttttgcgc aagcagaaga
    11401 gcaacagatt gttacaatca taacgaaccc catacttccc aaagctctct cttaacgtgc
    11461 gggagcttgt tggccttctt cctggcccat ctttttgaag aaccgatcgg aatcctgctg
    11521 aatgtccttt tttgacgggt ccgattgagg ttcgggtgtg gaagaacatc ccaccatcat
    11581 cgctcccatc aagaaaatag aacatatgat gacagtcacc tgagttgtgg cattactccc
    11641 attcatgatc gttcacctcc agcatcaatt gaacatatgt tgggtatcct tagaacagta
    11701 aagccatcct accctacatc ggacactgag catcgatcaa cacacagacc ggaattgact
    11761 gatttatagg tgtctggtac cgtcctggcc atcttcaaat gtcacgatca cacccttgat
    11821 accctactcg gtgcgatggc gggtgaagga atacggctca cgaaaatggg cattgaccaa
    11881 atcccacata gcggaaagcc tgatcaatta ctcgatcatt atgaaatcag ggcaagggcc
    11941 attgtcgata aagttaaaca attggtttaa gtatggcgac cgatgcctcc accatcaaat
    12001 tttgacttaa ttcccctctt tgcctgcctt tccgagtccg atcgaaggga actggcgaac
    12061 cacgcgatcg aagttcccat cacaaaagga cagtttatct ttcgggaagg tgatcccgcc
    12121 gactggtttc acattgtcaa acagggaaat gtaaaatgcg taaaatcctc accggaagga
    12181 cgcgaagtta cgctcaaggt tctcatgccc ggggatttgt tttgttgtga ggcatccgcc
    12241 ttttcaggag aaggtcatcc cgggtgcgcc caatccatgg gagaaggaac tattgtcaaa
    12301 attcaccgaa aggtgatgct ggatgtgatc caaagaaatc cgggtgcagc ggtcaatatc
    12361 atcaattact taagtctccg cctccgcgaa tcccaagaca atgccaaggc ctttgccttg
    12421 gaccgcgccg aacagcgggt cgcctccatt ctcgtcaacc tggcgcagcg atccggcatt
    12481 caagaggcaa ccggcattcg ccttgccgtg cgactgaccc atcaggattt agccgacatg
    12541 gccggcctga cgaaagaaac cacgagtcgc attctaagcc gattcaagaa agatcatatg
    12601 attagtggga caggaaaaaa gttggtcatc caagatctca ccagacttca gcagatggct
    12661 cctcatgcct tttcctagtt tctcctaaaa atacggcttg ccattcccgg ctctgagcca
    12721 acctccgggg acaccccgcg aaaatctttt cgcatttaat gatctatatc attgttttcc
    12781 tccggtattc ctctaagata aaaatgaata caaatgaaaa ggaccagggg ggctttttac
    12841 attttctcaa ttcattagaa ggaggaagtc atccgtgact catttagcgt tcacacactc
    12901 gattcgttca acaggtttca tctctctggc caccatgttg ggtgttttag ccatgggaag
    12961 cgtttcacca gaacccgcca tgagcatgcc aaccatccag gctcaactga cgacactccc
    13021 aaaagttcct accccagtta cccgtaagga agccgcgcat gttgtggtta atgtagaggc
    13081 aaaagaatat gtcgggacgt tagccgatgg cgttcaatac aaattttgga gttttaatgg
    13141 caccgtccca ggccccatga tccgcgtccg cgagggcgat accgtggaag tccaccttaa
    13201 aaatcatccg gagagcgttt ttccgcataa catggatctg catgcggtga atggtccagg
    13261 cgggggggca ggctccaacc tggctattcc cgggaaggaa gggatgtttg cctttaaagc
    13321 cttaaacccg ggactataca tttatcattg cgcctcccct gtcccgaata tcccggctca
    13381 tattgccaac ggcatgtacg ggttgattct tgtggatccg gctgaaggtc tgcctccagt
    13441 agataaggaa tttgcggtgt ttcaaagcga gttcttcacg atgcccagcg cagaaaaggg
    13501 catcatggaa ctctccatgg agaaaggctt agcggaacac cctgaccacg tggtgttaaa
    13561 cggaagagcc ggagccttga tcggggatgg gcagcttgaa gccaaggcag gagacaccat
    13621 caggctgtat gtcggcaaca tcggaccgaa tagcacatcc tcgttccaca tcattggcga
    13681 gatttttgac aaggtctatg tggaaggtgc cattggtgga accatcaata agaacgtgca
    13741 aaccacattg attcctgcag cgggagctac catcgtggaa tttaaagtgg atgtgcccgg
    13801 ggcgtacctg ttactggatc acagcatttt ccgggtcgcc aaaggggcag ttggcattct
    13861 ttccatcacc ggagaagaga atccttcgat ctataattcc atggatccag ccaactaagc
    13921 tttcttcgtt gtacattcaa gggcctgagt gagaacattc cttcaggccc tttttccttc
    13981 cagaatatcc accgccccac caattccaag attttagtct ctagccggga ttgggagcca
    14041 taaagaccag cacccgtagc cggttggtcg tatggttcac cacgccatgg tcttccccgg
    14101 cgggagccat cgtcccctgc ccttctccca ggacctggct ttcactcccg acccgaaacg
    14161 taccctcccc ttctaagacc aaatagactt tgtcctgatc ggcatgcaca tggccttttt
    14221 gttcctgccc gggctcgaaa ccatacacat cacaaaaaaa tctggatgat tgaaacacat
    14281 tctgcttctt catcttttcc ttcgaaaact ggccgtagtc tttgatattc accacattca
    14341 cttcagtgac ctcctcaatt gaacaatgcc gataccgccg ccaccattct ctcaatcatc
    14401 ctcaacaaat cccctacccc gaataatccc tcaccatccc ggacctcccc gaggcgattt
    14461 cacttcccag gagtgttgaa tactactaag ctccaagggc atattgaccc acaggcacgt
    14521 ggcatatcag aggcctcggg gcggttcaaa aaagcatgcc ctgatccttt cgaagggttc
    14581 gtccagccct gccctgagtt atgtcgaagg caaggccgca gtcatttttg cgcgcagagc
    14641 gtacttccag tatgtgagca cggaaaaatg gcgacctgcc tgcgcgaagc cgctccggcg
    14701 aaggcaggga acgccgctgg cggctttttt caacagcccc ttt
//
LOCUS       DATIAL010000344         6912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_815848, whole genome shotgun sequence.
ACCESSION   DATIAL010000344 DATIAL010000000
VERSION     DATIAL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815848"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1956)
                     /gene="mdoH"
                     /locus_tag="VLA60_10725"
     CDS             complement(<1..1956)
                     /gene="mdoH"
                     /locus_tag="VLA60_10725"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HSF09881.1"
                     /translation="MKFREATIAEAQHSLATPRRISWRIAAGLRRALLTVLVLIQSFV
                     AASYMTAILPYHGGNFLEMAIITLFFVLFVWISVGFWIGIFGFVLRCFGGDRFSPLGR
                     HTAAELSTQPLARTAVVMPIYHEPIQRTFGGIRAMYRSLEQTGQIDHFDFFILSDSRN
                     PDVWLAEQAAWHRLCTELGAGGHLFYRRRSLNQHYKSGNIADFLRRWGSGYEYMIVLD
                     ADSLMGGSTLVTMVQLMQAEPQTGILQTSPTLINARSMFARVQQFANHIYGPLFTTGL
                     ASLQLGEAVYWGHNAILRTQAFMKHAGLKRLPGPGLFHGPILSHDFVEASFMGRGGYE
                     VWLEPELSHSYEESPPTLVDDLQRDKRWAKGNLQHIWLMLFEPGLRPAHRMAFLNGIM
                     SYLASPLWLIFLVLTTIEAARLVLYPINYFPEPHSLFPHWPEWNPEWAIILASSTLFL
                     LFLPKFLSVIDVIRRRRSKQHGGIIRLLISVLLEIVVSTLLAPIRMLAHSRFVVEALL
                     NTTLRWAGQNRTDETGWAAAILNQAPGTLIAGAWAAFAFWLDQMFFFWSLPVAAPLVF
                     AAPTSVFLSRVGPGDRLKRWGFLQVPEERHGSPLLDDVYARTYPQLETYTERSSPAIQ
                     AILDPVLNQVHQAMAHAHRGGKKQEMIL"
     gene            complement(1953..2201)
                     /locus_tag="VLA60_10730"
     CDS             complement(1953..2201)
                     /locus_tag="VLA60_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09882.1"
                     /translation="MARSIAEHTLSRACDYLSAMGVELTREVTLRALTLVEAGLASKQ
                     EDPLQFVMTRIHDHFALQNPPLPTTAPPITRGSMSFKT"
     gene            complement(2201..3742)
                     /locus_tag="VLA60_10735"
     CDS             complement(2201..3742)
                     /locus_tag="VLA60_10735"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HSF09883.1"
                     /translation="MRILFAGMVAMFLMFPLHVAAGFTFDQVVQKAKNLADKPYEAPQ
                     LVPKIMREISYEAYKGIRFNPDHSLWKESQSNFQVMFLTPGLHYTHPVTINVIDAEGP
                     RPLVFKKSDFVFADPEIEKRVPADVGFAGFKLTFPLKNKNEQNQFLVFAGASYFRGVG
                     KENVFGLAGRGLAIDTGLPSGEVFPSFTEFWLVRPSPDAKEMVVYGLLDSISLTGAYQ
                     FTIVPGSQTKMKVRTKLFPRKPIQLLGVAPLTSMFFYGENTPRPAGEWRREVHDSDGL
                     QIHDGLTREWLWRPLMNPANLEMDFFSTDNVQGFGLLQREEKFSGYEDLGAHYEQRPS
                     AWVEPQGDWGQGKVVLVQLPTPNETHDNIVAFWTPVAPVTRETPLEMAYDVSFGKATL
                     SQNPLGTVVNTFIGDGNRIGGGNVPQAYRIIIDFAGGPLSKMPPDAPISGQVTVLDNS
                     DILEHFVEYHEQIQGWRLSILAKPQDKKPLHLRAFLKTDSATLTETWTYRLPMNNHIL
                     APEKE"
     gene            complement(4500..5600)
                     /locus_tag="VLA60_10740"
     CDS             complement(4500..5600)
                     /locus_tag="VLA60_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HSF09884.1"
                     /translation="MEHLLVALQQRNNPMISQGIIANPYGKPGGVQQTAYPDQGYQTT
                     QEYPAPQGYPSSPGYPQQPYDPYQSQQPQYTGNAPSPNNPYPDQEQQGNPYSTNPVYP
                     NQQASVPGYSSAPQNQYPQQSQQANAYPNTQTMYPNQQNPYPNQAPNPNQLPETPQYQ
                     TAQPETAQTGSVPISLDVLLVKKHMVNGVQSYLPIKDGDILLDGRGNPQAGDKFRVMF
                     RANTDCYVYVIAVDGSAWAQGIFPTASSPFANPIKAGQQYILPEGTNWFSLDQFKGIE
                     TIFVVASRAPRQDIEHILTTITGRERSPTEKPQAVSEPAIIPNGYSGARPGLSPFAFP
                     TAQNQQQELLPTTYFVKTAGEDLRITRWFRHQ"
     gene            complement(5831..>6912)
                     /locus_tag="VLA60_10745"
     CDS             complement(5831..>6912)
                     /locus_tag="VLA60_10745"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HSF09885.1"
                     /translation="GLPVQIDPYTGLPIQTDPNTGLPLQVDPNTGLPMENQQGFDPRQ
                     FPGDPNYGYGGAQPFNDGTPQYGFDANYPPVQGRGVPGQLPHARFPSTPCDQEANPQA
                     FQAGNSQNQLYGYDPNVAQQDYPQDSNAQGYTQADPYTQQTYPQDSYTQTGPETGVQT
                     QETGTPIGLDVIMVKKAIRNGAPVVLPIQDGDVLRDGRGNAAAGDKFRIMFRPNSDGH
                     VYVIAIDGSGWAQGIFPPPTAPLTNPVKAGEQYVIPEGNNWFSLDQFKGIETIFFVVS
                     QDKRQDIEEILQSITGHERPASENPHQVTKVAMVPNGVGGTRPSAKPFGLPEGSGPDH
                     AIIPTSYLAQQAGQDLRLTRWFRHE"
BASE COUNT         1621 a         1650 c         1925 g         1716 t
ORIGIN      
        1 gaggatcatt tcctgttttt tgccacctcg atgcgcatga gccatggctt gatgtacctg
       61 gttcagcacc ggatccagaa tagcctgtat ggcgggagaa gatcgttcag tgtacgtttc
      121 cagttgagga taggtcctgg catagacatc atccagcaat ggagagccat gacgttcctc
      181 gggaacttgc aaaaatcccc agcgcttcag tcggtcacct ggtcccaccc gactgagaaa
      241 cacggaggtg ggcgcggcaa acacgagtgg tgctgcgacc gggagggacc agaaaaaaaa
      301 catctgatca agccagaaag cgaatgcggc ccaggctccg gcaatcagcg tccccggggc
      361 ctgatttaaa atggcggctg cccagccggt ttcatctgtt cgattctggc ctgcccaacg
      421 taaggttgta ttaagcagtg cttccaccac aaaacggcta tgggccaaca tgcggatggg
      481 cgcgaggaga gtggacacca ctatttccag taagacacta atcaggagtc gaatgatgcc
      541 accgtgttgt ttgctccgtc gacgtcggat gacatcgatc acggacagaa attttggcag
      601 aaacagcagg aacaaggtgc tgctggccag aatgatggcc cattccggat tccattcagg
      661 ccaatgggga aacaggctat gaggttcggg aaagtaattg atcggataca agaccagacg
      721 tgcggcttcg atcgttgtta acaccaaaaa gattaaccac aagggggagg ccaggtagga
      781 cataatcccg ttcaaaaagg ccatgcgatg cgcaggccgc aggccgggct caaacagcat
      841 gagccagata tgctgtaaat ttcctttggc ccagcgttta tcgcgttgca ggtcatccac
      901 aagggtggga ggggattctt cataactgtg agagagttcc ggttccagcc agacttcata
      961 cccgccacgt cccataaatg aggcttccac aaaatcgtga ctgagaatag ggccatgaaa
     1021 aagtcctggt ccgggaagcc gcttcaggcc ggcatgtttc ataaacgctt gtgtgcgaag
     1081 aatggcgtta tggccccagt agactgcttc cccaagctgt agggaggcaa ggcctgtcgt
     1141 aaacagtggg ccgtagatat gattggcaaa ttgctggacc cgtgcaaaca ttgaacgggc
     1201 attgatcaag gtggggctgg tttgcaggat gcctgtttga ggttcagcct gcatgagttg
     1261 caccattgtg accagggtgg atccacccat gagactatct gcatcaagga cgatcatgta
     1321 ctcatagccg gaaccccacc ggcgaagaaa gtcggcaata ttcccacttt tataatgttg
     1381 gttcaaagac cggcgccgat aaaacagatg tccgcccgct cccaattcgg tacataaccg
     1441 atgccaagcg gcttgttcag ccaaccacac atccggattg cggctgtcgg aaagaatgaa
     1501 aaaatcaaaa tggtcaatct gcccggtctg ttctaatgag cgatacattg cgcggatccc
     1561 gccgaatgtc cgctgaatgg gttcgtgata gatcggcatg accacggctg tccgtgctag
     1621 gggttgagtg cttaactctg cagcggtatg gcgtcccagt ggtgaaaatc gatccccgcc
     1681 gaaacaccgg aggacaaatc cgaatatgcc aatccaaaat ccaacggaaa tccagacgaa
     1741 tagcacaaaa aacagggtga taatcgccat ctccaggaaa ttccccccat gatagggaag
     1801 gatggccgtc atatacgaag cggccacgaa agattggatg aggactaata cggtgagcag
     1861 ggcacgccgg agtcctgcgg caattctcca gcttattcgt ctgggggtgg ccagcgaatg
     1921 ctgggcttcg gcgattgtgg cttcacgaaa tttcatgttt taaaactcat gctgccccgt
     1981 gtgatcggtg gagcggtggt tgggagagga ggattctgca gtgcaaagtg gtcatggatt
     2041 cttgtcatca caaattgcag tggatcttcc tgctttgaag ccagtccggc ctcaacaagg
     2101 gtgagagccc gcagcgtgac ctcgcgggtt aattctactc ccattgccga caggtagtca
     2161 caggctcgtg agagagtatg ttcggcaatc gatcgggcca ttattctttt tctggtgcca
     2221 gaatgtggtt attcattggt aggcggtacg tccaggtttc cgttaaggtt gccgaatctg
     2281 ttttgagaaa tgcccgcaaa tgaagcggct ttttgtcttg aggtttggcc aggatggaca
     2341 gtcgccatcc ttgtatctgt tcatgatatt ccacaaaatg ctccaggatg tcgctattat
     2401 ccaacaccgt gacctggccg ctgatgggag catcgggcgg cattttgctc agtgggccac
     2461 ccgcaaaatc gataatgata cgatacgcct gcggaacatt tcctcccccg atacgattgc
     2521 cgtccccgat aaaggtattg accacggttc ccaacggatt ttgtgagaga gtggccttcc
     2581 caaaactcac atcataggcc atttctaaag gggtctcccg ggtcacgggc gctacgggtg
     2641 tccaaaatgc gacaatgttg tcatgcgttt cattgggggt aggcagttgg acgagcacca
     2701 cttttccttg cccccagtca ccctgtggtt cgacccaggc gcttggacgt tgttcataat
     2761 gcgcccctaa atcttcgtag ccagaaaatt tttcctcacg ttgcagtaaa ccgaatcctt
     2821 gtacattgtc cgtgctgaaa aaatccattt caaggttggc aggattcatc aagggacgcc
     2881 acaaccactc acgggtcagg ccatcatgaa tctgcaatcc gtccgaatca tggacttccc
     2941 tgcgccattc tccagctggt cgtggggtat tctctccata aaagaacatg ctggtcagcg
     3001 gggcaacacc caataactga ataggttttc tggggaagag tttggtgcgt accttcatct
     3061 tcgtctggct accggggaca atggtaaatt gatatgctcc agtgagactg atgctgtcca
     3121 gtaaaccata aaccaccatt tctttggcat ctggagacgg gcgtaccaac cagaattccg
     3181 tgaatgaagg aaatacctcc ccgctgggga gtcccgtatc aatggccagg cctcgtcctg
     3241 ctaacccgaa cacgttttcc tttccgaccc cccgaaaata actcgccccc gcgaaaacca
     3301 aaaactgatt ttgttcattt ttgtttttca aaggaaaggt cagcttgaat ccggcaaatc
     3361 ctacatcggc cggcacccgt ttttcgattt cggggtcggc aaacacaaag tcgcttttct
     3421 taaaaaccag cgggcgtgga ccttcggcgt cgatgacgtt gatggtcacc gggtgtgtgt
     3481 aatgcaaccc cggagtaaga aacatgactt gaaaatttga ttggctctct ttccatagac
     3541 tgtggtcagg attaaaccgg atccctttat acgcctcata ggatatttcc cgcataattt
     3601 ttgggacaag ttgaggggct tcatagggtt tatccgctaa gtttttcgct ttctgaacca
     3661 cctgatcgaa tgtgaagccc gctgcgacat gcagggggaa catcaagaac atcgcgacca
     3721 ttccagcaaa caagattctc atcactgacg ctcccgttta gtaagaagat tatgaaactg
     3781 aaactaccca tataaaatat cccactgagg aaaaaaccta agaaggaatg ctcaatgggg
     3841 aaggttattt tgcgttgcct aagagggaga tggtccccct gcttgatctg ggttccatcg
     3901 tgaattgtac tatatggggt aatgcaaaac tgagggaaag ggaggaattt atcgtaggac
     3961 aacccagaag cgagagacct tgatcgcttt ctatcattgg actttgtggg cagggaagcc
     4021 attggtgatg gtagcacgca aaaatatggt catggaaagt ttgagcaata ccttgaggca
     4081 attcacggct tagctgacta agtgagaata tgttcgtact caaggacatt ttggggccga
     4141 ttatagcaca tttttattgg gtatctatcg ggctctccgt tgtttttcaa gagtttttgg
     4201 ccatcgaccc acaaaattct ggaaagttat cgattgacag ctgcaattgc aagagaaacc
     4261 cagtcgccat cccagggtgc atattcagga gatgaacgtg aaaacgatca accgagaaaa
     4321 aagaacacgg cttatgaagt cttgaacaca ccgacaggat ctacaagtaa atgtttgact
     4381 gtcatgggtt gaccttgtcg ttaatcctga acccttttca gtttccatcg tgcctccggc
     4441 gtgaatttat tttcctgaag aatgtcgaaa gtgccttgtt gcttgaagca tccgtttgtt
     4501 tactgatgac ggaaccagcg ggtgatgcgg aggtcttcgc cggcggtttt gacaaagtag
     4561 gtggtgggaa gtaattcctg ttgctggttc tgtgcggttg gaaaggcaaa gggagataaa
     4621 cctggacgtg caccggaata cccattggga atgatcgcgg gttcgctaac tgcttggggt
     4681 ttttcagtcg gacttcgttc gcgcccggta atagtcgtca gaatgtgttc gatatcctgg
     4741 cgcggtgcgc gggaggccac cacgaagatg gtttcaatcc ctttgaactg atccaggctg
     4801 aaccaattcg ttccctccgg cagaatatac tgctgaccgg ctttgatcgg attggcaaac
     4861 gggctggagg ctgtcggaaa aatgccttga gcccaggccg atccgtctac cgcaatcaca
     4921 taaacgtagc aatcggtatt ggcgcggaac atcacacgaa atttatcacc ggcttgcgga
     4981 ttgcctcttc catccagaag gatatccccg tccttaatgg gcaggtagga ttgcacccca
     5041 ttgaccatat gttttttgac cagcagcaca tccaggctga tcggcacgga tccggtctgt
     5101 gctgtttccg gctgggcggt ctggtattgg ggagtctctg gtagttgatt cggattgggc
     5161 gcctgattcg ggtacggatt ctgctgattg ggatacatag tctgagtgtt cggataggca
     5221 ttggcctgtt ggctctgctg cggatattga ttctgtgggg cggaggaata tcctgggacc
     5281 gaggcttgtt ggttggggta gacaggattg gtggaatagg gatttccttg ttgctcttgg
     5341 tccgggtaag ggttattcgg agagggagca ttcccggtgt attgcggctg ctgactttga
     5401 taagggtcat acggttgttg agggtagcct ggagaggatg gatatccctg aggagcggga
     5461 tattcttgcg tggtctggta accctgatcg ggataggccg tttgttgaac gccgccgggt
     5521 tttccataag gattcgcaat gatgccttgg gatatcattg gattgttccg ttgttgaagg
     5581 gccactagca ggtgttccat gttttgttca tgggcgtccc cgtaattgtt aaccgctgtt
     5641 ctcaggaggt tggccccgaa ttctacgagt gggggaatta aaaaggggag cgccatacaa
     5701 ccgctgaaga gaaggctcgt acccatgagc agccagggac gtcgtggggg ggagtgagtc
     5761 attgaagagt tgaaggtcag gtgtatacca gccaactatt taatgaaatg acgccatgct
     5821 tatcttggcc ctattcgtgg cggaaccagc gagtgagccg cagatcttgc ccggcctgtt
     5881 gtgccagata actagtggga ataatggcgt gatccggacc tgacccttcc gggaggccga
     5941 acggtttggc gctcggccgg gttcctccga ccccattggg caccatggct actttggtca
     6001 cctgatgggg attttcggat gccggacgtt cgtgcccggt gatgctttgg aggatttctt
     6061 cgatatcctg ccgcttgtcc tgcgacacga cgaagaagat ggtttcaatc cctttgaatt
     6121 gatcaaggct aaaccagttg ttgccttcgg gaattacgta ttgctcgcct gctttcaccg
     6181 gattcgtcaa gggagccgtt ggcggcggaa agatgccttg cgcccagcct gaaccgtcaa
     6241 tggcgataac gtagacatgc ccatcgctgt tgggcctgaa catgatccga aatttgtcgc
     6301 ccgccgccgc attaccgcgt ccgtctctga ggacgtcgcc gtcttgaatc gggaggacca
     6361 ccggagctcc gtttcgaata gctttcttca ccatgatgac atccaatccg atcggagttc
     6421 ctgtctcctg ggtttggact cctgtctccg gtcctgtctg cgtataggag tcttgcgggt
     6481 aggtttgttg agtataagga tctgcttgtg tatagccctg cgcattcgaa tcttgaggat
     6541 aatcttgttg cgccacattg ggatcgtagc cgtaaagttg gttttgtgaa ttgccggcct
     6601 gaaaggcctg cgggttcgcc tcctggtcgc aaggggttga gggaaatctt gcgtggggaa
     6661 gctgccccgg tactccacgc ccttggacag gtggatagtt tgcatcaaat ccgtattgtg
     6721 gcgttccatc attgaagggt tgtgccccgc catacccgta gttgggatct ccaggaaatt
     6781 gtcggggatc aaacccttgt tggttttcca tggggagtcc ggtattggga tcaacctgga
     6841 gcggcaggcc ggtgttcggg tcggtttgaa taggaaggcc cgtgtaggga tcaatctgaa
     6901 ccggaaggcc cg
//
LOCUS       DATIAL010000345         3615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1138718, whole genome shotgun sequence.
ACCESSION   DATIAL010000345 DATIAL010000000
VERSION     DATIAL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138718"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..918
                     /gene="nadD"
                     /locus_tag="VLA60_10750"
     CDS             247..918
                     /gene="nadD"
                     /locus_tag="VLA60_10750"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HSF09886.1"
                     /translation="MFIGLLGGSFNPIHNGHLHIAQYVYNAIQLDRIIFIPTGDPPHK
                     PSASLAPAHHRLAMVQQAVAPFPFCTVSDYEIQSEAVSYSVDTVTHFKKECPEGTELG
                     FIVGLDAFLDFCSWRQVDHLLTLCHFIVCSRPGVAFTQLQSLPFLPAAKSQGFQSLDQ
                     QDTTRLDILLPSGKKLYLLAVPPCEISASTIREQLAGGSRVSQWLPPTVESYIIRHRL
                     YQWPV"
     gene            906..1346
                     /gene="rsfS"
                     /locus_tag="VLA60_10755"
     CDS             906..1346
                     /gene="rsfS"
                     /locus_tag="VLA60_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HSF09887.1"
                     /translation="MARLTHPKPSPTLEQAVFIAQAAQEKQAGEVLVLDVGAMTSIAD
                     YFVFASGDSERQVRGIASFIEKVMSTRYELSPDIEGKETANWILLDYGDIIVHIFKSD
                     VRKYYALESMWADAPQISIPESRKPFPQVAQAPKAPGKVARLSS"
     gene            1420..2436
                     /gene="bioB"
                     /locus_tag="VLA60_10760"
     CDS             1420..2436
                     /gene="bioB"
                     /locus_tag="VLA60_10760"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HSF09888.1"
                     /translation="MTPFQDLADRVLEGTIPSREEGLAILDTPQEKLLDLVNAAYRIR
                     SRYFGNTIRLQMLLNAKSGACQEDCHYCSQSTISTAPIERYALVSPEKMLEGARRAAQ
                     AKAQRYCIVISGRSPLQSEITHISSAVKQIKEELPIQICCSLGLLNGEQASQLKAAGV
                     DRINHNLNTSEAYHPAICTTHTYQDRLDTLKHARAAGLELCSGGIVGMGERDEDLVDL
                     ALALQDIQPDSIPLNMLYPVEGTPFDEMKNLTPVRCLKILCLFRFFHPKTEIRAAGGR
                     ERNLRSLQPLALYVADSLFVDGYLTTPGLAHQEVWKMIEDLGFTVEVKYEGIEAEKVE
                     TCSS"
     gene            complement(2488..>3615)
                     /locus_tag="VLA60_10765"
     CDS             complement(2488..>3615)
                     /locus_tag="VLA60_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HSF09889.1"
                     /translation="PGRTSVTMAAIVVGLAIMVGVGIMIQSFRNTVDIWIEQTMLADI
                     IVAPVSWLGEQEIESDKPSLPLALVRTVMMVPGVEAVDPYMETMGEVAGESVSLVARD
                     MALHAARSQYLFVAGDSTLILRETIEGEGVIVSEVLAGRLGLAVGSHIEVKTPSGQQA
                     FPVKGIFYDYATDGGKVVMDDQLFRRYWGESDATVFAVYLKAGQSLPVVRHEIEQVLK
                     NDLPIVTISNGELKTEILEIFDRTFRVTYVLELITLSVAVLGIINTLMTAITERRREL
                     ATLRALGMSRPQIQGLIFWESCYVAGLGAGLGILVGLALSVLLINVINKQSFGWTIQF
                     TLPWETLGMAVLVALLAAMLGAWGPARWAGRQVIAEDLRYE"
BASE COUNT          888 a         1055 c          871 g          801 t
ORIGIN      
        1 cagacctgtc ctgaggccat cgaaggaaag gccgcagcat tttggacgag cggaacatac
       61 ggtggagtat gagcacggcc aaggagcgac ctgcctgcgc gcttgcgcgt cgaagcgtgc
      121 ttcaacgcgt agacgcgcag ccgctttcag ccttcgtagc tacttcggcg aaagaggccc
      181 ggcgaaggca gggggaacgc cgctggcggc ttttcaacac gctccacatg agaaaaatct
      241 agactgatgt ttatcggcct cctaggcggc tcattcaatc ccatccataa tgggcatttg
      301 catattgccc agtatgtcta caacgcgatt cagcttgatc ggatcatttt cattccgacg
      361 ggggatcctc cacacaaacc atcggcctcc cttgcgccag cccatcaccg gttagcaatg
      421 gtgcagcaag cggttgcccc gtttcccttc tgcaccgtat ccgattacga aatccaatct
      481 gaggcagtgt cgtacagtgt cgacaccgtc acacatttca aaaaagaatg ccccgaggga
      541 acggagttgg ggtttattgt ggggcttgat gcttttctgg atttctgcag ttggagacag
      601 gtggatcatt tgctcaccct gtgtcatttt atcgtctgct cgagaccggg ggttgccttc
      661 acacaacttc aatccctgcc gttcctacct gcggcaaagt ctcaaggatt tcaaagcctt
      721 gaccaacagg acacgacccg cctggacatc ctgctcccat ctggaaagaa actttatctc
      781 ctagcggtac caccgtgcga aatctcggct tcgacaattc gagagcaact ggccggtggt
      841 tcccgcgtca gccagtggtt gccgcccacg gtggagtcct atataattcg ccatcgattg
      901 taccaatggc ccgtctaacc cacccgaaac catctcctac cctcgaacag gcggtcttca
      961 tcgctcaagc ggctcaagaa aagcaagccg gagaggtgtt ggtgcttgat gtgggagcaa
     1021 tgacgtcaat agcagactat ttcgtctttg cctcagggga ttccgaacgc caggtgcgag
     1081 ggattgcctc cttcattgaa aaggtcatgt ccacacgcta tgaactaagc ccggatattg
     1141 agggaaaaga aacggccaat tggatattac tcgactatgg agacatcatc gtccatatat
     1201 ttaagtcaga cgtccggaag tattatgcct tagaaagtat gtgggcggac gctccacaaa
     1261 tttctattcc tgaaagccgt aaaccttttc cgcaggtcgc acaagcgcca aaagcccccg
     1321 gaaaagtcgc caggctttct tcttaagcaa cgagaaggtt ttgtcttttt atccgtaggt
     1381 tggaaatcga atttttcagg aaaaataatg gagaacccca tgaccccatt tcaagatttg
     1441 gcagaccgcg tgctggaggg aacaatccca agccgggagg aaggcttggc cattctggac
     1501 acccctcagg aaaaactgct cgatctggtc aatgcggcct atcggattcg gtcacgatat
     1561 tttggcaaca ctatccgcct gcaaatgttg ctcaatgcca aaagtggagc ctgtcaggaa
     1621 gattgtcact attgttccca atcaaccatt tccactgctc ccatcgagcg gtacgcactc
     1681 gtctctccgg aaaaaatgct ggaaggcgct cgacgggccg cacaggccaa agctcagcgg
     1741 tattgcattg tgatcagtgg ccgctccccg ttgcaatcgg aaatcacaca catcagcagc
     1801 gccgtcaaac aaattaaaga ggaactgccg attcaaatct gttgttcact agggctcttg
     1861 aatggcgaac aggccagcca gttaaaggcg gctggggtcg atcgcatcaa ccataatctc
     1921 aataccagtg aagcatatca ccctgctatt tgcacgacac atacctacca ggatcgtctc
     1981 gacaccctga aacatgcccg cgcagccgga ttggaactct gtagcggcgg tatcgtcggc
     2041 atgggtgaac gggacgaaga tctggtggat ctggccctgg cgttacagga catccaaccc
     2101 gattccattc ccttaaacat gttgtacccg gtcgaaggca caccatttga cgaaatgaaa
     2161 aacctcacac cggtccgttg cttaaaaatc ctctgcctct tccgattctt ccaccccaaa
     2221 acggaaatcc gggcagccgg aggacgcgaa agaaatctcc gctcgctgca acccttggcg
     2281 ctctacgtgg cagactccct cttcgtggat ggctatttga ccacgccggg ccttgcccat
     2341 caggaagtct ggaagatgat cgaagacctg ggctttaccg tggaagtaaa gtacgagggg
     2401 attgaagccg aaaaagtcga aacctgctca tcctgattgc tctacctgcg gtgtcctaat
     2461 attcctttat ccatagacac ctccgagtca ttcatatctc aaatcttcag cgatgacctg
     2521 acgaccggcc catctggctg gcccccacgc ccccagcatt gcggccaaca gcgccacaag
     2581 gacagccatt cccaacgttt cccatggcag ggtgaactga atagtccagc caaatgattg
     2641 cttattaatg acgttgatca acaacaccga cagcgctaat cccaccagta tgcctaagcc
     2701 ggcacccaat ccggccacat aacaggattc ccagaagatc aggccttgga tctgtgggcg
     2761 actcattccc aacgcgcgta gcgtggccag ttcccgacgg cgttcggtga tggccgtcat
     2821 gagggtattg ataatcccca gcacggccac gctcagggta atcaactcca acacataggt
     2881 gacgcgaaac gtccggtcaa aaatttcaag aatctcggtt ttcaattctc cattactgat
     2941 tgtgacgatg gggaggtcat tttttaacac ctgttcgatc tcatgtcgca cgaccgggag
     3001 cgactgaccg gccttgagat acaccgcaaa gacggttgcg tcagactccc cccaataccg
     3061 acgaaagagt tggtcgtcca tgaccacttt tcccccatcg gtggcataat cataaaaaat
     3121 gcccttaacc ggaaatgcct gttgtcctga aggcgtcttc acttctatgt gacttcctac
     3181 cgccagcccc agccgcccgg ctaatacctc agacacaatc acgccttcac cttcaatcgt
     3241 ttctcgtaaa atgagagtgg aatcacctgc cacaaacaga tattggctgc gcgcggcatg
     3301 aagggccatg tcgcgtgcga ccaaggacac cgactcccct gcaacctcac ccatggtttc
     3361 catgtaggga tcaaccgcct cgacccctgg gaccatcatc actgtcctga ccagagccaa
     3421 tggcagagag ggcttgtcgc tttcgatctc ctgttccccc aaccatgaca ccggcgccac
     3481 gatgatatcc gccagcatcg tttgctcaat ccagatgtct accgtattcc ggaaactctg
     3541 gatcatgatg cccactccca ccatgatggc caacccgacg acaatcgccg ccatggtcac
     3601 actggtccga ccggg
//
LOCUS       DATIAL010000346        10277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_493641, whole genome shotgun sequence.
ACCESSION   DATIAL010000346 DATIAL010000000
VERSION     DATIAL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493641"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VLA60_10770"
     CDS             complement(<1..1313)
                     /locus_tag="VLA60_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF09890.1"
                     /translation="MAEMGSSSRSNGAEIILDCISDGVITIDLQKRVTFLNRAMQKML
                     GYNVDAAGTLLACDVLIQSNICSTKECVLERALQGERVSNFEAMVRRRDGVHIPVSIN
                     TDFLLDKEGKLIGLIEVIRDISLVRELSAKVEEVSELKHRLGEQTKLDNMVGRCPRMQ
                     DIMAKLPIIAASKSSVLITGESGTGKELIACALHAHSPRKDAPFVVVNCSSLSEGILE
                     SEIFGHVKGAFTNAYFDKPGRFEIANGGTIFLDEIGEMSVATQVKLLGVLERGQFERV
                     GSNDTVTVDVRVVAATNRNLEEAVQQGRFREDLYFRIRVIPVVLPPLRERTGDIPLLV
                     NHFLEKFNREMGKHIVSLSPQCLTALSRYPFPGNIRELQNMIEHAFVCCEEDTIQFEH
                     LPADLHRYCWEHREWTDSESLEALERQAICRALEKTGWRLKEASQ"
     gene            1652..2470
                     /gene="lgt"
                     /locus_tag="VLA60_10775"
     CDS             1652..2470
                     /gene="lgt"
                     /locus_tag="VLA60_10775"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HSF09891.1"
                     /translation="MIALDPVSILADLARIPYPEIDPVFFRIGPLALRWYGLMYLLGL
                     AGAYWLIKSRAATRHVSLPPQQLSDLIVYAAFGVFLGGRLGYVLFYNLPFYLDHPLKI
                     FAVWEGGMSFHGGLIGTCIAIWIFCKTRGFPVWPIADLAAGAAPIGLGFGRLGNFING
                     ELFGRSTDVEWCMVFPHGGPECRHPSQLYEAGLEGVALFVILTILNRRPTPPGTMFWA
                     FISGYGVARFFVEFFREPDSHLGFIFQWITMGQLLCIPMVAVGVFMIVRGYKQA"
     gene            complement(2522..2971)
                     /locus_tag="VLA60_10780"
     CDS             complement(2522..2971)
                     /locus_tag="VLA60_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09892.1"
                     /translation="MRMCVGVYFIMMAAGGCASDKPAPLTALAPPPNVDIQVLQVMTE
                     GKRLFDEGRWEAARQQFQFAVQQQADLAEAHYNLALCMDKMGDQAGAKKHFIEAANLA
                     PGNKVIWNSPPLRRYGNVPDAPTQATSAPVIPGFGGGATPGGGGRGF"
     gene            complement(3004..6447)
                     /locus_tag="VLA60_10785"
     CDS             complement(3004..6447)
                     /locus_tag="VLA60_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HSF09893.1"
                     /translation="MSNPERLPDADARLMAETTFDRNVVVLAGAGTGKTTLLVNRLIH
                     ALLREPHSLRLTDMLALTFTNKAANEMKARLRDRLHGLLADCEAEAGSEDSGGTDLEE
                     FKQRYHVSTAHVREKIQIALRDLEKSQIATLHSFAAHVLRLYPLEARVDPHFHEDEGT
                     QFLETFQHAWDAWLEQELGMQGSAHAQWQDLLNHMGLQEIRSFAFSLCQDQISIPELD
                     QQVCAEGSSPAFRKWLQSKQHQVRSLLTQYDRSKPRKIEKLLSLAQRVFNRVGYGEPP
                     IDFHLSDEEKALVDSTIGKAPGEWDASDFQDARRAIQAAQQLLQVNEVLLSKVLHVLG
                     PFVERVRQDFSAKGWIRFDGLLIRVRDLLRDHPMVREQLKRTYAAIMVDEFQDTDPVQ
                     YEILLYLGECPNESGRFWRDIQLMPGKLFIVGDPKQSIYAFRRADIEAFDQVVDKVTH
                     DGGIVCTLLTNFRSDGAILETVNAVFDRLFIPQANVQPPNVPLAVGRMREAGSGSTGM
                     EICVMANSQGEDEWDAERATRVEAEWLAGWIEEQLLPGSQWIMEKGVRTALRPGHIAV
                     LLRKFTNAQVYLEALQRHNIPCMADGERHFYRRQEVIDVVNVLRVLDDPTDALALVGI
                     LRSSLGGLTDREIMDVMKLGPLDIRQPQRLGAWASSQQSVIQGLFQRLAWLHAQANRL
                     PLPELLDHLFQQLPLVELAAASSHGEQAVVNVWKLRDLMSEQAAIPSLSFSAWVERLV
                     DSLMSHPSEPEAPLAEETLDAVRVLTIHKAKGLEFPVVLLPGLHQKATGLDRGAQITF
                     DWISGLYGCTLPPVWNAGQVPLWEKQRIREAAEQRRLLYVGMTRARERLILSGGILPK
                     VGGESLLSLIQGIVEGEFGNPELDIVRVGEVSIPHTVVTPAVLKYLKSPQHQADPEDS
                     SEVVISTPQWDTREMRWQQTNQAEAYVNPSMLHQARLVQDPGERGQGSRGTGQRLGIL
                     MHRVLQRWNFQSEPGRVRSALVDFCERQLPGQSGLNKSEVVRELATLLDLFLGSPGYR
                     ELQRATILGREVPFAVPWPVGQHEPSLPRTCVMEGVMDVVYEIDGDVWVGDYKTDHVS
                     SRTVGPRAETYREQAQAYARAARQCLGPAVKGCKLFFIRLGEVVTVTVETDGNMFQHE
                     Q"
     gene            complement(6440..9691)
                     /locus_tag="VLA60_10790"
     CDS             complement(6440..9691)
                     /locus_tag="VLA60_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HSF09894.1"
                     /translation="MFTLVSGPFSPHLESALVETVRQIKSADSRAPLAILVPSDSLRR
                     RLQWLLCAEHSCALFDLHVLTFHQFALHLHAEQAALDSLETGMSPFELVGDFFYEYLL
                     AVLLEKSGQTVGPFADLQGSSGLRQAVWRSIRDLLEAQVEPRLALRAVEEGLFDEIAV
                     TRLAGLMNLQEAIGNLSRQLGVGLPEDLANSVIPWVGASPFVARLSTVMYYGFYDITQ
                     VQLSLLEEVARTNSVKVFFPLTDQTAYQFAQRFLDRHLLKAEVVYQPLQVRHEPFELA
                     NRTGSSPSVQVVNVIGAQGELVFTCKAILHAVETTGHTFHEIGVVARTLDPYGLFLWR
                     VFEEHRIPFCTTATLPLLEEPVAKLWWQLAGLREERYPWQALLNVVTSPYYRSLSVNG
                     CSTHEQKPLWSQAIRHWRLMQGREDWERLAAVANDPEAIGDWQRKVGVPLEEASVAIQ
                     QCVDVVGRLVADCQALPESGPIGELTLAFEALVNTHLSLLQEETSSQMEARDQAHQTS
                     LAQAFEQVMTQLKQLDRVGCQVTWGAWVEVFRGALEGTRLPVPGQSPLGVQVFDAMAA
                     RGHAFRTVFILGMNDQVFPRVVREDAFLRDGDRRVLAESLGYKIDEKMHGFDEEALLF
                     ALLRHSARDRVYLVYQRADHKGRPLLPSPLLTEWARDVPGCSESEISVPLRVAERVRI
                     PYFSPGEETGQEMRLRNLLQGRAIQTDSLEPSFWWKLFRHGLKAVAALERTSTGGGSF
                     DGIMPVEGAHWQELLSRGLSPTALERYAQCPFRYWMEHALRTRNVREPISREMPNRIW
                     GELGHAILRHVYRYLIDHKWPVSPIEPVHFSSLIASTIDQVCDKYSTHYGKGYVVLWE
                     RMKTQLGAVVFAMIEHDQQEYVDQAMVPVDCEVGAEGEIVSEVPGGSTLLKIHGRVDR
                     VDRQSDGSRTRIVDYKFSGGLTTRTDDPDLVNEALRGRRLQPPLYSLMSSVSLSGHSG
                     EHPKEMILPHPAMHSVEFRFIRPLHPAPLTFSSFHGSIWATQTGTQLLQAMRRWIEGI
                     RTGQFFVLPGSYCRDCSWSVACRSQHHPSWVRAYGIPLAKELRQGRKQRATHE"
     gene            complement(10005..>10277)
                     /locus_tag="VLA60_10795"
     CDS             complement(10005..>10277)
                     /locus_tag="VLA60_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSF09895.1"
                     /translation="TLLVSKSCSACPNAKTLWKGMRIKYSFSYREIDITSEAGQELAE
                     RHAIRAVPATLINGRLTFIGVPPRESAEKALQAKAKPKPKKEETEE"
BASE COUNT         2459 a         3039 c         2422 g         2357 t
ORIGIN      
        1 tgttgagaag cctccttcag ccgccaaccc gttttttcca atgcccggca aatagcttgc
       61 cgttccaagg cttccagtga ttcagaatcg gtccattccc gatgctccca acaataccga
      121 tggaggtcgg ctggaagatg ctcaaattga atcgtatcct cttcacagca cacaaaggca
      181 tgctcaatca tattctgaag ctccctgata ttgccgggaa acgggtaccg gctgagtgcc
      241 gttaaacatt gcggggacaa gctgacaatg tgcttaccca tttcgcggtt gaatttttcg
      301 agaaaatgat tcacgagcag gggaatatct cccgttcgct cccgtagggg gggcagcacc
      361 accggaatca ctcgtatacg aaaatacaaa tcttcccgaa accgtccctg ctgaacggct
      421 tcttccagat tccgattcgt ggccgcgacc actctcacat ccaccgtgac cgtgtcattg
      481 gatccaaccc tctcaaactg tcctcgctca agcactccca ataattttac ttgggtggcg
      541 acactcatct ccccgatttc atcaagaaag atcgtcccac cattcgcaat ttcaaaccgg
      601 cccggtttat caaaatacgc attggtaaac gcccctttga cgtgaccgaa tatttcactc
      661 tccaaaatgc cctcagatag acttgagcaa ttcaccacca caaaaggggc atcctttctc
      721 gggctgtggg cgtgtaacgc acaggcaatt aattccttgc ccgttccact ttctccggtg
      781 atgagaacgg aggatttcga agcggcgatg attggcaatt tcgccatgat gtcctgcatg
      841 cgaggacatc gacccaccat attatccagc ttggtctgct ctcccagccg atgcttgagc
      901 tccgacacct cctccacctt ggcacttaac tcgcgaacca gcgagatatc acgaatgact
      961 tcgattaacc cgatcagctt gccctcctta tccaataaaa agtcggtatt gatgctcacc
     1021 ggaatatgta cgccatcacg tcgacgaacc atcgcttcaa aattcgacac ccgctctccc
     1081 tgaagcgccc gctccaggac acattccttg gtcgagcaga tattgctttg gatcaggaca
     1141 tcacaggcta agagtgtccc tgccgcatcg acgttatacc ccagcatctt ctgcatcgcg
     1201 cgattcaaaa aggtcacacg tttctgcagg tcaatcgtaa tcaccccgtc actaatacaa
     1261 tccagaatga tttctgcacc attcgaacga gacgatgatc ccatttcagc catgacgtct
     1321 atgcctctcg tcaaagtacc gacttccgat tacagcccac atgtcttttc tttcatccgc
     1381 atttatggta gttagatgct gtgatgtctt ccgaccaacg tgaccggacc cgtttcctgt
     1441 tagtgtaagg caaccctatt ctgctccaca aacccttgcc gatagccatt ttgccacttt
     1501 ccaagaagaa cgaggttctc ccaaccggtt cgcccctggt gaagcatgcg gttcgccttc
     1561 ttcccattcg gccaccaatc ggggggtcgc ctttagatgg agcacaaacg tcttccgggc
     1621 accgccgtcg tgaggtaagg cacgttaccg gatgatcgcc ctcgaccctg tctcaatttt
     1681 ggcggacctc gcgcggatcc cctacccgga aatcgatccg gtcttttttc gtatcgggcc
     1741 cctggcactt cgatggtatg gcctcatgta cctcctggga ttggcgggag cctattggct
     1801 catcaagtcc agagccgcca caaggcatgt ctctctcccg ccacaacaat tatccgatct
     1861 tattgtctat gccgcctttg gcgtctttct cggaggccgc ttgggatatg tgttgtttta
     1921 taacctgccc ttttatctgg accatcccct caaaattttt gcggtatggg aagggggaat
     1981 gtcctttcat ggcgggctga tcggaacgtg tatcgcgatc tggatcttct gtaaaacccg
     2041 tgggtttcct gtttggccca tcgccgactt ggccgcagga gcggctccaa tcgggttggg
     2101 gtttggacgg ttggggaatt ttatcaacgg agaattattc gggcggtcga ccgacgtcga
     2161 atggtgcatg gtctttcccc atggcggccc ggagtgccga cacccctcgc aactctatga
     2221 ggccggattg gaaggtgtcg cactctttgt catattaacg atactcaatc gccggcccac
     2281 gccaccggga accatgttct gggccttcat ctccggatat ggagtcgctc gattttttgt
     2341 agaatttttc cgtgaaccgg atagtcattt agggtttatc ttccagtgga tcacgatggg
     2401 tcagttgctc tgtatcccga tggttgcggt cggcgtcttc atgattgttc ggggctacaa
     2461 acaggcataa cccccccttc acgcatgctt atcagattcc gggaaatccc caaaaagcag
     2521 cttaaaaacc cctacccccg ccgcctggag tcgctcctcc accgaatcct ggtatgaccg
     2581 gagccgaggt ggcttgcgta ggggcatcgg gaacattgcc ataccgtcga agcggtggag
     2641 aattccaaat caccttatta ccgggagcca ggttggcagc ctcaatgaaa tgtttctttg
     2701 cgccggcttg atcgcccatt ttgtccatgc acagcgcaag attatagtgg gcttcggcca
     2761 gatcggcttg ttgttggaca gcaaactgaa actgttgtcg tgctgcttcc caccggcctt
     2821 cgtcaaagag gcgcttgcct tcggtcataa cctgcaacac ttggatatcc acatttggag
     2881 gaggggccaa ggcagtcaac ggcgctggtt tatcagatgc gcaccctccc gccgccatca
     2941 taatgaaata gaccccaacg cacatcctca tcctccaatt cctcattgct ccctccttgt
     3001 catctattgt tcgtgttgaa acatatttcc atcggtttct acagtcaccg tgaccacttc
     3061 cccgagacga ataaaaaaga gtttacaccc tttgaccgca ggacccaagc attggcgagc
     3121 ggcccgcgca taggcctgcg cttgttcccg gtaagtttcc gcgcgcggcc ccaccgtgcg
     3181 agaggatacg tgatccgttt tataatcgcc aacccacacg tcgccatcga tttcatagac
     3241 cacatccatc accccctcca tcacgcaggt ccgcggcaag gaaggctcat gttgcccgac
     3301 aggccatggc accgcaaatg gaacttcccg accaagaatc gttgcccgct gcaattcccg
     3361 ataccccggt gaaccgagaa agagatcaag gagcgttgca agctctctga ccacctcact
     3421 cttattcaac cctgactggc ctggtaactg gcgttcacaa aaatccacaa gcgcacttcg
     3481 tacccttcca ggttcacttt gaaaattcca ccgttgaagc acccgatgca tcaaaatccc
     3541 aagacgctga ccggtcccac gtgaaccttg acccctctcc cccgggtcct gaaccaatct
     3601 cgcctgatgc agcatagagg gattgacgta agcctctgcc tgattggtct gttgccatct
     3661 catttcacgt gtgtcccact gtggcgtcga gatcaccacc tcactactat cttcaggatc
     3721 agcttggtgc tgtggggact tcaaatattt caacactgcg ggcgtcacga cggtgtgagg
     3781 aatggaaacc tctcccaccc gtacaatatc cagctccgga ttcccgaatt ctccttcaac
     3841 aatcccctga ataagactca aaaggctctc cccacccact ttcgggagta tgccccctga
     3901 taagattaag cgctcccggg ccctggtcat tccgacatat agcagacgtc gctgttcggc
     3961 ggcttctcga atccgttgct tttcccacag aggcacctgg cctgcattcc acacaggtgg
     4021 caacgtacaa ccgtacaagc cgctaatcca gtcaaatgta atctgtgcac cgcgatccag
     4081 tcctgtggcc ttctgatgca acccgggcaa cagcaccacg gggaattcga gtcctttggc
     4141 cttatgaatc gttaggacac gcaccgcgtc taacgtctct tcggccaaag gggcttccgg
     4201 ttctgaagga tgggacatga gggaatcgac caatcgctcc acccaggcag aaaacgacaa
     4261 agaaggaatg gccgcctgtt cactcatcag gtcacgaagc ttccagacat tcaccaccgc
     4321 ctgttcacca tggctcgagg cggcggccaa ttccacgagg ggaagttgtt gaaacaggtg
     4381 atcaagtaat tccggaagag ggagtcgatt tgcctgggcg tgaagccagg ccagccgctg
     4441 aaaaaggccc tgaatgactg actgttggga actcgcccat gcaccaagtc tctgaggctg
     4501 ccggatatcc aacgggccaa gtttcatcac atccataatc tccctatccg tcagcccgcc
     4561 taacgaagaa cggagaatcc ccacaagggc cagagcatcg gtggggtcat ccaggacgcg
     4621 aagcacattc accacatcaa tcacttcctg acgccggtaa aaatgccgct caccatcggc
     4681 catgcaagga atgttgtggc gctgcaacgc ttcaagatac acctgcgcat tggtaaactt
     4741 tcttaacaac accgcaatat gcccgggacg taaggcggtt ctcactccct tctccataat
     4801 ccactggctt cccggcagca attgttcttc aatccaccct gctaaccatt cggcctccac
     4861 ccgtgtggcc cgctcagcat cccattcgtc ttccccttga gagttggcca ttacacagat
     4921 ctccattcct gtcgatccgg aacccgcctc tcgcatccgt ccaaccgcca gggggacatt
     4981 agggggttgg acattggctt ggggaatgaa taaccggtca aacaccgcat tgacagtttc
     5041 aagaatggcc ccatcactac gaaaattggt cagaagagta cacacgatcc ctccatcatg
     5101 cgtcactttg tccacgacct gatcaaacgc ctcgatatca gctcgccgaa aggcatagat
     5161 tgattgcttg ggatcaccca caataaacag cttccccggc ataagctgga tatcccgcca
     5221 aaacctcccg gactcatttg ggcactcacc tagataaagt aaaatttcat attgaacggg
     5281 atcagtatcc tgaaactcat caaccattat ggccgcatag gtcctcttca attgctctct
     5341 gaccatggga tgatcccgta ggagatctct aacccggatc aagagcccat caaatcgaat
     5401 ccatcccttc gccgaaaaat cctggcgaac tcgttcgaca aacggcccca gcacatgaag
     5461 aaccttactc aagaggactt cattgacctg caacagctgt tgcgcagctt gaatcgcccg
     5521 cctggcatct tgaaaatccg acgcatccca ctcccccggg gccttgccaa tggtggaatc
     5581 aaccagggct ttctcctcat cagacaaatg gaaatctatt ggtggctccc catagcccac
     5641 ccgattaaac acgcgttgag caagagataa gagcttctca atttttcgag gtttggatcg
     5701 gtcgtattgc gtcagcaatg atcgaacctg atgctgttta ctctgtagcc acttccgaaa
     5761 ggccggcgag gacccttcgg cacacacttg ctgatccaat tcaggaatag aaatctgatc
     5821 ctgacagagc gaaaaggcaa aggatcgaat ctcttgcaaa cccatgtgat tgagcaggtc
     5881 ctgccactgg gcatgcgcgg aaccttgcat acctaattcc tgttccaacc aggcatccca
     5941 cgcatgttga aatgtctcaa ggaattgagt gccttcatcc tcatggaaat gaggatcgac
     6001 cctggcttct aagggataca gacgaaggac atgagcggca aaactatgga gcgtggcaat
     6061 ttgcgatttc tccagatcac gcaaggcgat ctgaattttt tctcttacat gcgccgtgga
     6121 cacatgatag cgttgcttga actcctccag gtctgtgcca ccggaatcct cacttccggc
     6181 ttccgcctca caatcagcca gtagcccatg caaccggtcg cggagccgcg ctttcatttc
     6241 attcgcagct ttattcgtga aggtcagagc caacatgtcg gtcaagcgta acgagtgtgg
     6301 ctccctcaga agcgcatgaa tcagtcgatt caccaacagc gttgttttac ccgtcccggc
     6361 tccggccagc acgacgacat tgcgatcaaa cgtggtttcc gccatgagtc gcgcatcggc
     6421 atcgggaaga cgttcgggat tactcatggg tggcccgttg ctttcgtcct tgccgcaatt
     6481 ctttcgccaa gggaattccg tacgccctca cccacgacgg atgatgctgc gaccgacagg
     6541 ccaccgacca cgagcagtcc cgacaatagg aaccaggtag aacaaagaac tgcccggtgc
     6601 gtatgccttc gatccaacga cgcatggcct gcaacaattg ggtacccgtt tgcgttgccc
     6661 agatcgaacc gtgaaacgat gagaaggtga gaggcgcagg atgtaatgga cggataaacc
     6721 ggaattccac ggaatgcatc gcaggatgtg gcaagatcat ttctttggga tgctctccag
     6781 aatgaccgga caggctcacg gaagacatca gtgaatataa aggaggttgt aaccggcgac
     6841 cacgcagggc ttcattgacg agatcggggt cgtctgttcg agtcgtcaat ccacccgaaa
     6901 atttataatc cacaatacga gttcgtgagc catcggactg gcggtccacg cgatccactc
     6961 gcccatgaat cttcagcagg gtcgagcctc ccggcacctc gctcacaatc tctccctcgg
     7021 ccccgacctc acaatccacc ggcaccatgg cctgatcgac gtactcctgc tgatcatgct
     7081 cgatcatcgc aaataccaca gcacctaatt gcgttttcat tctttcccac aggaccacat
     7141 acccttttcc atagtgcgtc gaatatttat cgcagacctg atcaatggtc gaggcgatca
     7201 aggaagagaa atgaacgggc tctattggag agaccggcca tttatgatcg atgagatagc
     7261 gatagacatg tcgaagaatg gcatgcccca actcccccca aatccgattt ggcatttccc
     7321 gcgaaatggg ctctcgaaca ttccgtgtac gcagagcatg ttccatccaa taccggaaag
     7381 gacattgggc atagcgttcc aacgctgtcg gtgacaaccc ccgggacaag agctcctgcc
     7441 aatgcgcccc ctcgaccggc atgatcccat caaatgagcc tccgcctgtg gacgttcttt
     7501 caagagcagc caccgctttg agtccatggc gaaacaattt ccaccagaac gatggctcca
     7561 aagaatctgt ttggatcgcc cgtccctgaa gcagattccg caggcgcatt tcctgtccgg
     7621 tctcttcacc cggagaaaag tatggaattc tcacccgctc agccacacgc aacggcacgc
     7681 tgatctccga ctcagaacag cccggtacat ctcgtgccca ttccgttagg agaggagacg
     7741 gcagtagcgg gcgccccttg tggtcagcac gttggtagac aagatacacc cggtcacgag
     7801 ctgagtgtcg aagcaacgca aacaagagag cctcttcatc aaacccgtgc attttttcat
     7861 caattttata tccaagactt tccgccaaca ctcttcgatc cccgtcccgt aaaaaggcat
     7921 cctcccgaac cacacgagga aagacctgat cattcattcc gagaataaac acggttctaa
     7981 aggcatggcc tcgcgcggcc atggcatcga acacttgaac ccctaagggg ctttgtcccg
     8041 gaacaggcag tcttgtccct tccaaggctc ctcgaaacac ctccacccat gccccccagg
     8101 tcacttggca accgacccga tccagttgct ttaactgcgt catgacctgt tcgaatgctt
     8161 gtgcaagact tgtctgatgg gcttgatccc gcgcttccat ttgagaagat gtctcttctt
     8221 gaagcaagga aaggtgggta ttcacgagtg cttcaaacgc gagggtcagt tcaccaatcg
     8281 ggcctgattc cggaagagcc tgacaatcgg ccaccagtcg acccacgacg tccacgcact
     8341 gttgtatagc gacagacgcc tcttccagcg gcactccgac cttccgctgc cagtcaccaa
     8401 ttgcttccgg gtcattcgcg acggctgcaa gacgttccca atcctcccga ccttgcataa
     8461 gacgccaatg acgaatggct tggctccaga gtggcttttg ttcatgggtg gaacatccgt
     8521 tgaccgacag gcttcgataa tagggagagg tcacgacatt cagcagtgcc tgccaggggt
     8581 accgctcttc cctcaagccg gcaagctgcc accaaagctt ggcgaccggt tcttccaaca
     8641 gaggcaaggt cgctgtagtg cagaagggaa ttcgatgctc ctcaaacacc ctccacagaa
     8701 atagcccata tggatcgaga gttctggcca ccaccccaat ctcatgaaac gtgtgccctg
     8761 tcgtttccac cgcatgcaga atggctttgc aggtaaaaac cagctcccct tgggccccaa
     8821 tcacatttac cacctgcacg gacggggatg accccgtccg attcgctaat tcgaacggtt
     8881 cgtgcctaac ctgaagaggt tggtacacca cttcggcttt taatagatgt cgatcaagaa
     8941 atcgttgggc aaattgatag gcagtttgat cggtaagggg aaagaacacc ttcacggaat
     9001 tcgtacgagc cacttcttct aacagcgaaa gctgaacttg cgtgatgtcg taaaaaccgt
     9061 aatacatgac cgtcgagagt ctggcaacaa acggtgatgc cccaacccag ggaatcaccg
     9121 aattggccaa atcttccggc aatcccaccc ctaattgtcg gctcaaattc ccgatggcct
     9181 cctgaagatt cattaagccc gcgaggcgag tgaccgcaat ctcatcgaac agcccctctt
     9241 ccaccgcacg gagagccagg cgtggctcaa cttgcgcttc tagcaaatct cgaatgctcc
     9301 gccacacggc ctgcctgaga cctgatgaac cttgaagatc agcaaagggt ccgacagttt
     9361 gcccactctt ttccaaaagt acggctagaa ggtattcata gaaaaagtcg ccgacgagtt
     9421 caaacggaga catccctgtt tccagggaat ccaatgccgc ttgctcggca tgaagatgga
     9481 gagcgaactg atggaacgtc agaacatgga gatcgaagag cgcacaggaa tgctcggcgc
     9541 acaataacca ctggagcctt cttcgcaatg aatccgaagg cacaagaatg gccagcggcg
     9601 cgcgagaatc ggccgatttg atctgccgga ccgtttcgac aagagctgat tcgaggtgag
     9661 gagagaatgg gccgctgaca agcgtaaaca tccggacttt tgcgttcgat cggaggtagg
     9721 ctacaagacc gatcctgccg atttctaacc ggtttctggt cctatgagtt cgccattaca
     9781 atcagggcaa ctccaatccc cgtcaatcag ggacatggga tcaccacaga aggaacattc
     9841 aggtggagga ccggaaggaa ccctgggaag gacgggcaac tcaatatcta attctatttc
     9901 gtcttcatct tcattgggca tgtgaaaacc tcgtctatct ttcgcggtcg ttatctactc
     9961 agaaattgtc tatgaggagc tgtcgtagag atgaagtcct gaaattattc ctccgtttcc
    10021 tcttttttag gttttggctt cgccttcgct tgcagagctt tctccgcact ctcccgcggt
    10081 ggaactccga taaaggtcaa ccgtccattg atcagggttg cgggcacggc acgaatagca
    10141 tgtcgctccg ccagttcttg accggcctcc gatgtaatat caatttcccg gtaactgaaa
    10201 ctatatttaa tccgcatgcc tttccagagc gttttcgcat ttgggcaggc cgaacaggat
    10261 ttggacacca acaaggt
//
LOCUS       DATIAL010000347         3155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_924745, whole genome shotgun sequence.
ACCESSION   DATIAL010000347 DATIAL010000000
VERSION     DATIAL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924745"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1059..1919)
                     /gene="trpA"
                     /locus_tag="VLA60_10800"
     CDS             complement(1059..1919)
                     /gene="trpA"
                     /locus_tag="VLA60_10800"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HSF09896.1"
                     /translation="MSNRIQATFAQLHSKGEKALIPYIMAGDPTLAMTESLVLALEQG
                     GADLIELGVPFSDPIADGPVIQQAAERALHSGTTLKNILATVANLRKKTSIPLILMTY
                     YNSLMAMGIKDFCANAVDAGIDGLIVPDLPPEESDLLYHATQAAGGPVSIFLLAPTST
                     AERRRAVIQRSHGFIYYVSLTGITGAKLTDLSLVRQSVQQLQRASRKPVAVGFGIASA
                     EQAREVGRFADGVIIGSALVRYISEHHSAPTFLTNIQGMAHQWKSVLLPVTANRRPSV
                     AKRHKVPAPS"
     gene            complement(2000..>3155)
                     /gene="trpB"
                     /locus_tag="VLA60_10805"
     CDS             complement(2000..>3155)
                     /gene="trpB"
                     /locus_tag="VLA60_10805"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HSF09897.1"
                     /translation="FGGKYAPEILMPAIHELEQAYRAAKKSRPFQQEFLNCLKHFVGR
                     PTSLFFAERLTKTLGGAKIYLKREDLCHTGAHKINNTVGQALLTRRMKKNRVIAETGA
                     GQHGVAVATVAAMFGLEAEIYMGTEDMERQALNVFRMRLLGSKVTGVNSGSRTLKDAI
                     SEAMRDWTTNVRTTHYLLGSVLGPHPYPMMVRDFQSVIGRETRKQILALEGRLPHCLV
                     ACVGGGSNSIGLFYPFVKDTQVQMVGVEAGGYGIESGKHAARFAGGKPGVLHGTMTYL
                     LQNEDGQVNPTHSVSAGLDYPAVGPEHSYYHDTGRIRYTSATDAEALAAFDLLAKEEG
                     IIPALESAHAVAEVVKLAPTMKKSQILIMNLSGRGDKDVQQLAKMRGIPL"
BASE COUNT          740 a          767 c          840 g          808 t
ORIGIN      
        1 gaacaacccc ttgttaatta tggacgtttt ggcatgtcga tgctttctac tagaccaaga
       61 accatgccag aagtttcgga tgccgcatgg aataaaatcc ttgaaaacca gttacatttt
      121 accttttttt gtgttgtgtg aattttgaca ccaggcaagg actgcccggg ctaggtaaaa
      181 aagatttgct ggattttccc tggataatga caaaaggagg aaggcagata tcttcctcta
      241 ctctggaagg agccaaatga aatagggggt tgttgaaaaa aaagccgcca gcggcgttct
      301 cgccattttc ccgtgctcac gtactggaag tacgctctgc gcgcaaaaat gaccgtggtc
      361 ttgctggacg aattttttga accaccccga ggcctctgat atgcaacgtg ccagtgggtt
      421 aataacctcc tggaaattat tattatcaac actcccaata gggcaatgcg acagcaacct
      481 gccggttcat tgttcttggg ctcattatca gggaatgcaa agtggtttgt tcccaagggt
      541 tttcagctta gggttgtacg tgtgcaggtc ctcaatggag ttcgatctaa tagagcagac
      601 tgccctacga ccagatgggc tgaggcattg gcccagaggt ttacctacct ggctaacgag
      661 gattgggcga ttcgtccggc tgtatagaaa ggccaaatag gatggttgga tggttccacc
      721 atttgtggga gtctctcaat cgttgacctt gtttccctct agccggtgaa ttgaacgtcg
      781 aagcggtgaa gaattagtga aagatgcagg gcgcgcctga ctgaccgccc ccttccttgc
      841 tgaccttcga gtggaaactt tttggaaatt tttctggtag gagccgtctc accttcctgt
      901 ttttaaggaa gttcatgaag cctccctgcg tcgaaaagat ccgtcccggg atgaggaaag
      961 gggtgattca attgtgaggc aatcgtggca gtgagagccg ccgacaatca ggaatgactc
     1021 tgtcgattat gggcattgaa ggtgctattt gaacccactt acgaaggggc ggggacctta
     1081 tgccttttgg ctacggatgg tcgacgattt gctgtgacgg gcaagaggac ggatttccat
     1141 tggtgggcca tgccctggat attggtgaga aatgtcgggg cagaatggtg ttcactaatg
     1201 tatcgaacca gggcgcttcc aataataact ccatccgcaa atcgtccaac ctccctggcc
     1261 tgctcggcgg aggcaattcc aaatcccact gccacgggtt ttcgtgaggc tcgttgaagc
     1321 tgttggacgc tttggcgcac cagactgaga tcggtcagct ttgcaccggt aataccggta
     1381 agagagacat aataaataaa accatgtgat cgctgaatca cagcacgtcg tcgttccgcc
     1441 gtgctggtgg gagcaagcag aaaaatactc accggtcctc ccgccgcttg agtggcgtga
     1501 taaagcagat cagattcttc cggaggcaaa tccggcacga tgagtccatc gatgcccgcg
     1561 tcaacggcat tggcacaaaa atctttgatc cccatggcca tgaggctgtt gtaataggtc
     1621 atcaaaataa ggggaataga cgtctttttt ctcaggttgg cgacagtggc aagaatattt
     1681 ttaagggtag tccctgagtg cagggcccgt tctgctgctt gttgaatcac cggaccatcg
     1741 gcgataggat ccgaaaatgg cacccccaat tcgatcaggt cggcaccacc ctgttccaac
     1801 gctaatacaa gagattccgt catcgctaag gtcggatcgc cagccatgat gtatgggatg
     1861 agggcttttt cacccttgga gtggagttgt gcaaatgtgg cttgaatgcg attgctcatg
     1921 tcagaacatt tcaaatgtgc cggaggacct ggtggccttc gtggcctggt gattctctgt
     1981 ccactgagta tgccctttcc tagagaggaa ttccgcgcat tttggctaat tgttgaacat
     2041 ctttatctcc gcgcccggag agattcatga ttaagatctg tgactttttc attgtgggtg
     2101 ccagcttgac gacttctgca accgcatggg cactttctag tgcgggaatg atgccttctt
     2161 ccttggcgag taagtcgaac gcggctaagg cttcagcatc cgttgcggag gtgtaccgga
     2221 ttcgtccggt atcatgatag taactgtgtt caggaccgac tgccggatag tccaatccgg
     2281 cagacactga atgggtggga ttgacttgac cgtcctcatt ctggagaaga taggtcatcg
     2341 tgccatgcaa aacgccgggt ttccctcctg caaagcgcgc ggcatgtttc ccgctttcta
     2401 tcccgtaccc gccagcttcc acgccgacca tttggacttg agtatctttg acgaacggat
     2461 aaaataatcc aatgctattg ctgccaccgc cgacacaggc gacaagacag tgaggcaaac
     2521 ggccttccag tgcaaggatt tgctttcggg tttctcgacc aatcacggat tgaaaatccc
     2581 gaaccatcat ggggtacgga tgggggccca ggaccgatcc aagcaaatag tgagtcgtgc
     2641 gtacattggt ggtccagtca cgcatcgcct cactgatggc atccttgagt gtgcggcttc
     2701 cggaattcac ccccgtcact tttgatccaa gtaagcgcat tcggaagaca ttcaatgcct
     2761 gtcgttccat atcctcggtg cccatgtaaa tttcggcctc aagaccgaac atggcggcca
     2821 cagtagcaac cgctactcca tgctgtccgg caccggtttc ggcaatgacg cggttctttt
     2881 tcattcgtct ggtaagcaga gcttgcccga cggtattgtt gatcttgtgc gcaccggtat
     2941 gacagaggtc ttctcttttg aggtaaatct tggctccacc aagtgttttg gtgagacgtt
     3001 cggcgaaaaa aagagaagtc ggacgaccga caaaatgttt caggcaattc agaaattcct
     3061 gctggaaggg ccggcttttc ttagccgcac gatacgcctg ttccaattca tggatggcgg
     3121 gcatcaggat ttccggagca tacttgcctc cgaat
//
LOCUS       DATIAL010000348         4389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1570895, whole genome shotgun sequence.
ACCESSION   DATIAL010000348 DATIAL010000000
VERSION     DATIAL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570895"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(427..2022)
                     /locus_tag="VLA60_10810"
     CDS             complement(427..2022)
                     /locus_tag="VLA60_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HSF09898.1"
                     /translation="MNTNRIRQRTRRIPRGIVVGLFGLLHALSLPVWASDTIVVGSKN
                     FMESRLLAEMFAQLIEARTELTVTRRLGLAGTQVCFEALKTGGIDLYPEYTGTGLVTI
                     LGEPPMHDRVSVLNRVRSEFLHRWNIWWLAPLGFENSYALALRRDRAQTLGLRTISDL
                     VRVAPHMKAGFGYEFIQREDGLAGLKKSYGLQFQEVIGMQQTLKYQATDKGEVDLLDV
                     YTTDGRLAVYDFVVLKDDRQFFPPYEAAALVRGEILKRHPELGRVISLLTNAIDPTRM
                     RELNLRIQEQGEPVPQVAHSALRALNLVADNHVQPAASMESSDSILSYMWTKRSNLAL
                     RTGEHLGLSGLGLFLGILVAIPLGLALERWRRGAETIIRIIGMLQTIPSIALLAFMIP
                     IFGVGALPAIMALWMYSLFPILRNTYSGLRDAAPNVVETGRALGMTEWQILRTIRLPL
                     AAPTIMAGIRTSAIWTVGTATLAAFIGAGGLGVPIVAGLQLADVNLILSGALPATVLA
                     LSVDGLLSRVEQWARPRGLEPVR"
     gene            complement(2033..2782)
                     /locus_tag="VLA60_10815"
     CDS             complement(2033..2782)
                     /locus_tag="VLA60_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HSF09899.1"
                     /translation="MNPWALEIQHVSKQFPDGTYALQNIEWSVKKGETMALIGESGSG
                     KTTFLRLLNRLIEPTTGTVVIQGEPASAQDPIQLRRRLGYVPQDGGLFPHWTIKQNVC
                     LVPHLLGWTLEQQLERVDRLLPLMNLNPSQIAERYPIELSGGQRQRVAVARALAADPP
                     IVLLDEPFGALDPLTRHDLQDQFLSLQARLQKTMVLVTHDMSEAFRLGDRITILKEGC
                     IQQVGTPHQILHAPATPYVHLLVQHYHAHRG"
     gene            3567..4028
                     /locus_tag="VLA60_10820"
     CDS             3567..4028
                     /locus_tag="VLA60_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09900.1"
                     /translation="MNVNSLTGRDVASIDLETENRNGKHPEHEPDLSDSGKCIVVLGV
                     DLKTWTGLTENLWDLGFTLKRLPSSFQGLDQLNNAFVEGILWDCESSSLTGLAVVSQI
                     RGRMHVPPVMVISSPSNKRLLIKALENGAMDFIMKPIDHMELRNKCVRLFG"
     gene            4073..>4389
                     /locus_tag="VLA60_10825"
     CDS             4073..>4389
                     /locus_tag="VLA60_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF09901.1"
                     /translation="MIPVWVLNSQNLSPENPFIRVKSKKKLDGKNSESLRPVSILVVD
                     DSVDCRMILRMALEREGYRCIEAANGIAALKLFQEKPIDFIITDFQMPHMDGCEFLEV
                     ISSA"
BASE COUNT         1104 a          969 c         1210 g         1106 t
ORIGIN      
        1 tgtggtgaca tgagccctcc tcgggggggt ctatcgtgaa caatagtcta accgctacct
       61 tcagacgtca gcgcagacca cgtcatcgtt ggaataaggc ggaaagaaaa tgttcaggaa
      121 acggaaagaa aatgttcaga aaaatgaaca tcctttggtc taaatttttc tcgtgtaatg
      181 ctgaatgaga gaaagcattc gttcgataag ttccttgatc tgcctgcaat gcgcaaaact
      241 aaagggcagt aggtggatga tggcgaatat attgaaaatt tatcaatatc tccactatag
      301 cggcagagag ggttgcgggc aagataaggg cattgaccaa atcagatgag tacgtggctc
      361 ttttgtccgc caaaatgtca cttgggttgg cgtgtttttt atatattaaa cggcaattcg
      421 gtaaggttat cgaaccggtt ccagacctcg tggccgggcc cattgttcta ctcgactcaa
      481 aaggccatca accgacagag ccaacacggt ggcgggtagg gcgccggata agatcaaatt
      541 gacgtccgcc agttggaggc cggccacgat ggggacaccc aagcctccgg cgccgataaa
      601 ggcggccagt gtggcagtcc cgacggtcca gattgcagag gtgcgaatgc ccgccatgat
      661 ggttggcgcg gcgagtggaa ggcgaatcgt gcggagaatt tgccattccg tcatgccaag
      721 ggctcgcccg gtctccacaa catttggagc tgcgtctcgc aaccccgaat aggtatttcg
      781 aagaataggg aataacgaat acatccataa ggccataatg gcggggaggg ctcctacgcc
      841 aaatatcgga atcataaagg ccagaagtgc gatggatgga atcgtttgca acatgccgat
      901 tattcggatg atcgtctccg ctccccttcg ccatcgttcg agagccagcc ccaatggaat
      961 ggcgaccagt atgcccagga acaaccctag tccggaaagc ccaagatgtt ctcctgtccg
     1021 aagggccaga ttggaacgtt tggtccacat gtaggacaaa atggaatcgg aagattccat
     1081 gctggcggct ggttggacgt gattgtcggc cacaagattg agtgcccgta aggcgctatg
     1141 cgctacttgg ggaaccggtt ctccctgttc ctgaatccgg agatttaatt cacgcatgcg
     1201 tgtcgggtct atggcattgg tgagcaaact gattacccgt cccaattccg gatggcgttt
     1261 gagaatttct cctcgaacga gtgccgcggc ctcatacggt gggaagaact gacgatcatc
     1321 tttcaggacg acaaagtcat acacagctaa gcgtccatct gtggtgtaga catctaagag
     1381 gtcaacctct cccttatctg tggcttgata tttcaaggtc tgttgcatgc cgatcacttc
     1441 ctgaaactgt aaaccgtagg atttttttaa ccccgccaat ccatcttctc gctggataaa
     1501 ttcatatcca aatccggctt tcatgtgagg ggcgacacgc acaagatcag aaatcgttcg
     1561 gagtcctaag gtttgtgctc gatcacggcg aagggctagc gcataagaat tctcaaatcc
     1621 gagtggtgcc agccaccaaa tattccatcg gtgaagaaac tccgagcgaa cgcggttcaa
     1681 aacagacact cgatcgtgca tcggcggttc accgagaatg gtgacgagcc ctgttcctgt
     1741 gtattcggga taaagatcaa tccctcccgt ttttaaagcc tcaaagcaga cttgagtgcc
     1801 ggcgagtccc aggcgacgcg tgacggttaa ttccgtgcgg gcctcaatga gttgagcgaa
     1861 catttccgcc aggagccggc tttccataaa attcttggat cccaccacaa tagtgtcaga
     1921 ggcccaaacc ggcagggaaa gagcatggag cagtccaaaa agtccgacca cgattcctct
     1981 agggatcctc cgtgtgcgtt gtctgatccg attggtgttc atctccttcc tgttaacctc
     2041 gatgagcgtg ataatgctga acgagcaaat gaacataggg ggtggctgga gcatggagaa
     2101 tttggtgcgg agtcccgacc tgttgaatgc atccttcttt gaggatggta atccggtcgc
     2161 ctagccggaa ggcttctgac atgtcatggg tcaccaggac catggttttt tgcaatcggg
     2221 cttgaaggga gagaaattgg tcctgcaggt catggcgggt gagcggatct aacgcgccaa
     2281 atggttcgtc gagcaggacg atgggcggat cggctgccag ggcacgtgcc acggccacgc
     2341 gttgccgctg tccccctgag agttcaatgg gatagcgttc ggcaatttgc gagggattca
     2401 ggttcatgag aggtaagagc ctgtcgactc gttcgagttg ctgttccaat gtccatccca
     2461 ataaatgagg gacgaggcag acattttgct tgatggtcca gtgagggaaa agcccgccat
     2521 cttgtgggac ataccccagg cgtcgacgaa gttgaatggg atcctgtgca gaggcgggct
     2581 cgccttgaat gacgacggta ccggtcgttg gttctatcag ccgattcaga agacgcaaaa
     2641 aggtggtttt tcctgatccg ctctcaccaa tcagcgccat ggtttcaccc tttttcacac
     2701 tccattcaat gttctgaagg gcataggtgc catccgggaa ttgcttgctg acatgttgga
     2761 tttccaatgc ccacgggttc atgtgggtga ggtccttagg gacggggata agaggatcca
     2821 aatggtgtgg tgatgcgtca tcatcatgat gaacgtctgg agaattacct gtgacctgtg
     2881 gccaggataa cagggttcaa ctggagagac tagcctcgtt atcaaggccg gatgccatgc
     2941 tggcttagga aatccttcta taaatttgga gagaaaggcg aagaaagggg agcaaatggg
     3001 ttatttcgct catgctaacc tgtgcgccaa cgctcggttt tttcatcata gtgacttgat
     3061 cttctggctt tggaatgaag tgctgaaatg tatggagaaa tttcggagat attggaatgt
     3121 ataagatggg catagtggtt gcactacttg atgagaaaaa tagaagagat ctggatgcaa
     3181 gaaattttga atgctctctc atcatcataa tgatgttatt tttatgggtg tgctttttac
     3241 tttcctagga gattcgaatg catgcacaca gaaatggaaa ttcttatctt gagggctgcc
     3301 cgacttctag ccaagaaccc gatgcctgtt gtcgatgtgg gggactcatg aaaagcgagt
     3361 cagtttttga actcgttgaa aatgaaattg aattcatgtc cgctcgctgt ctgcagtgtg
     3421 gcgatattgt ggatccggtt attctcatga atcgatccgg gaaaggccta ttgagtagtc
     3481 gcaccggaag ggctcggcat tgaatcttcc catactttgt tcgaggagtg gattgagata
     3541 ggccgcagtc cagaaagcat catccaatga atgtgaatag cttgaccggg cgtgatgtgg
     3601 cgagtatcga cttagagaca gaaaatcgga atgggaagca tccggagcat gagcctgacc
     3661 tttcggacag tggtaaatgc attgtagttt tgggtgtcga tcttaaaaca tggactgggt
     3721 tgacggagaa tctttgggat ttagggttta cgcttaaacg tcttccctct tcctttcagg
     3781 gattggatca actgaataat gcattcgtcg agggcatatt gtgggactgt gaatcgagtt
     3841 ccctgacggg cttagctgtt gtgtctcaga ttcgcggaag aatgcatgtc ccgccggtta
     3901 tggtcatctc gagtccttca aacaagaggc tgctgataaa agcccttgaa aatggggcga
     3961 tggattttat tatgaaaccc atagatcaca tggaactccg aaataaatgt gtgcggttgt
     4021 ttggataggg agtgcgatgg gtgaaggagg acaaatttag ggaggcatgt ccatgattcc
     4081 tgtatgggtt ctcaattccc agaacctttc tccggaaaat ccatttattc gtgtgaaatc
     4141 gaagaagaaa ttggatggga aaaattctga atctttacgt cctgtttcta ttctggttgt
     4201 cgacgacagt gtggattgtc gcatgatact ccgcatggcc ctggaacgag agggctatcg
     4261 ttgcatagag gcagcgaatg gaattgctgc tctcaaatta tttcaggaaa aaccgattga
     4321 tttcatcatc acagatttcc agatgccaca tatggatgga tgcgaattcc ttgaagtcat
     4381 ttcgagtgc
//
LOCUS       DATIAL010000349         4206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_709805, whole genome shotgun sequence.
ACCESSION   DATIAL010000349 DATIAL010000000
VERSION     DATIAL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709805"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..1849
                     /locus_tag="VLA60_10830"
     CDS             539..1849
                     /locus_tag="VLA60_10830"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HSF09902.1"
                     /translation="MQTNESQDELEVSGEEEELSPEEYLKPLLQRAKQAARPLSGLIA
                     MKKNAALMAMADGLEAGEEAILKANEEDLSAFEGNPSRTAMADRLRLTPARIADMAAH
                     IREIAQLRDPVGESMGFWQRPNGMRVGRVRVPIGVIGIIYESRPNVTADAAALCLKSG
                     NVCVLRGGSEAHHSNLAIATILGEAAQKSGIPEGAITFIDKTDREVVLALLKSHRFVD
                     LIIPRGGEALMDTVTQHATIPVIKHDKGVCHIYVDSDVDLAMAQRISFNAKAQRCSTC
                     NSMETLLVNEKVAKKFLPPLAEEFIEAGVEMRGCSKTCQIISQAKPAQEDDFGKEFLS
                     LIVAIKVVKDMESAMDHIHQYGSRHTDSILTNDYDRALRFLREVDSSTVMVNASTRLN
                     DGYEFGLGAEIGISTTRIHARGPMGLEDLTCSKYVVYGSGQVRE"
     gene            1964..3745
                     /locus_tag="VLA60_10835"
     CDS             1964..3745
                     /locus_tag="VLA60_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09903.1"
                     /translation="MNLQCVLNHPQALRFPANQIYRQEWESAGGRILEQPTGHCVLYG
                     KHGQRILLADPEGNPLHECLWEQKPTGALSLVSARLRLDWGQWVGIKPKGLVNTISLD
                     LSRRPGWEQITQDDLRQMAARSLQSDLAMVRFFYRDEDVVLHGNGQATIHQVKDAFYV
                     LPNGSFEEARFMSCMSRMEWGRIDYLPVVELFLSLLPGTGSATFEFIRGLYDDQNNNG
                     GRVLQYRGIPAYPSDAAFRLFSLFFTPSVASGQSPLEVFLDMERSHEVHWLPASSFPV
                     RYMDEEQHVCVTVRNQMIQKVTWWDDPSGLPFNRIPESGLPVSDNRGAGVTADGLWLF
                     DGPGQKELTIRPSWQLSKPNHSVSWKPLASTWRDGFPRKPPMLTPQEAFSSVLLYPDK
                     SEMIGERESQPFIFDFLDDFLEEHQDLFHLRSHAKRVLLSHCEAGLGSCLQFQDNQIH
                     IIWYTWPQFAQKHAQSIWNRLSRMDRLAWFSNFQFIPFERLMLKTLPTRYDWIYLWIP
                     FSDYSNSNMIDCWVKFLRTYLAEGSVGCVAGPPVLKENFQRLGIQILYSAAGDSLPPF
                     RIHQTILPNGRLNPDLAVWIIQNPVQS"
     gene            3984..>4206
                     /locus_tag="VLA60_10840"
     CDS             3984..>4206
                     /locus_tag="VLA60_10840"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HSF09904.1"
                     /translation="MNTSPRPHERLLMDGQEMARTLTRMSHEILERNKGIEGLALVGI
                     RTGGVFLAKRIALRIQQIEKRDVPLGELDI"
BASE COUNT         1015 a          985 c         1082 g         1124 t
ORIGIN      
        1 cctaagtgtt cggattcaga aggtttcgac tctttatgaa tggtttccgg accgattcga
       61 aaaaaaatga tccgatttta cccgctaggg aaaagctaag atttgatggc aacgttggca
      121 gttagtttca aaagtggtga gatgcgttat gagttgcact gatgagcgag tcaaaaacaa
      181 ccgttatctt gaaatttcag gcaacaggtt ttggttttct gacatcatac attttaagaa
      241 ttaaccgcta cggtccctgg taaatcctct ttcttctaca tggcggcctt tatcgcaaaa
      301 aagacggacg gccgcggtag gagaaaccta tggagcaact ccactcatat tttttgaaaa
      361 gaaggtagcc cctccttaat gtgatacatg ctcgattaag tcgtgcacaa tgttggtagg
      421 caggaagtca ttgtcccctc agtccaatcc tcgcttcctt gacggttttt gtaactgcca
      481 ggtattctca acactattcc gtacataaaa tattgttacc tcaggaaaac ataaggctat
      541 gcaaacaaat gaatctcaag atgaacttga agtttcagga gaagaggaag agctttcccc
      601 tgaggaatac ctgaaaccat tgcttcagcg agccaaacag gctgcccgcc cattgagtgg
      661 attgattgcc atgaaaaaga atgcggcgtt aatggctatg gctgatgggc tggaggctgg
      721 ggaagaagcg atcctcaagg ccaatgaaga agatctttca gcattcgaag ggaatccctc
      781 acggacggcc atggcagatc gacttcgcct aacgccagcc agaattgccg atatggcggc
      841 gcacattcgc gagattgcgc agttacggga cccagttggc gaatcgatgg ggttctggca
      901 acgacccaat ggaatgagag tcggtcgggt gcgtgtgccg attggggtga tcggaatcat
      961 ttatgaatcc cgccccaatg tgaccgcgga tgcagcggcc ctctgcctca aatccggcaa
     1021 tgtgtgtgtg ctgcgagggg ggtccgaggc gcaccattcc aatttggcta ttgcgacgat
     1081 tcttggggaa gcggcccaaa agtctggaat tcccgaagga gcaattacct ttatcgacaa
     1141 gactgaccgc gaagtagttc ttgctctgct caaaagtcac cggtttgttg atctcatcat
     1201 tcctcgtggt ggcgaggcgc tgatggacac ggttactcag cacgcgacta tcccggtgat
     1261 caagcacgac aaaggcgtat gccatattta tgtggattca gatgtggatc tggctatggc
     1321 ccaacgaata agtttcaacg cgaaagctca gcgttgctcg acctgtaaca gcatggaaac
     1381 ccttctggtg aatgagaaag tggcgaaaaa attcttgcct cccttggccg aagaatttat
     1441 tgaggctggg gtcgaaatgc ggggatgttc gaaaacctgc cagatcattt ctcaggccaa
     1501 acctgcccaa gaagacgatt tcggcaaaga atttttatct ctcattgtgg cgatcaaagt
     1561 ggtgaaggat atggagagcg cgatggacca cattcaccaa tacggttccc gtcatacgga
     1621 ctctattctg accaatgact atgatcgggc tctacgattt ttgcgagagg tggattccag
     1681 tactgtgatg gtcaatgcct cgacccgttt gaacgatggc tatgagtttg ggttgggtgc
     1741 cgaaattggg attagtacaa ctcgtattca tgctcgtggt cccatggggt tagaagattt
     1801 aacctgttcg aaatatgttg tatacggttc cggtcaagtc cgggagtaag ggcccgatcg
     1861 gttcgctgca gtgtttccaa tttatgtttc tcacacccat cccgtctccg gttttggttt
     1921 tttttagagg cgatctgcct tctaagcttt tccatgcctc atcatgaacc ttcaatgcgt
     1981 attgaatcat ccccaagcct tacgttttcc tgccaatcag atctatagac aggagtggga
     2041 gtcggccggt gggcgtatcc tggaacagcc cactggtcat tgtgtgttat atggcaagca
     2101 tgggcaacgg attcttttgg cagatccaga gggaaatcct ctccatgaat gtttgtggga
     2161 gcaaaagcca accggggcgc tatcccttgt ctctgcccgt cttcgattgg attggggtca
     2221 atgggtcggg attaaaccga aagggttggt caataccatt tccttagatt tatctcggcg
     2281 gccaggctgg gagcagatca cccaagacga cctgcgtcag atggcagccc ggtccttgca
     2341 atccgacttg gcaatggtcc gatttttcta tcgggatgag gatgtcgtcc ttcatggtaa
     2401 cgggcaagcc acgattcacc aggttaaaga cgccttttat gtgttaccca atggatcctt
     2461 tgaagaagcc aggttcatgt catgtatgag tcggatggag tggggtcgga ttgactatct
     2521 cccagtggta gaattgtttt tatccctttt gccgggaacc ggtagtgcga cctttgaatt
     2581 catccgaggt ctctacgacg atcaaaataa taatggtggc cgcgtcctgc aataccgggg
     2641 tattccggca tatccctctg atgcggcgtt tcggctcttc agcctatttt tcaccccttc
     2701 cgttgcttcc ggacaatccc cgctggaagt gtttttggat atggaacgat cacatgaagt
     2761 ccactggctg cccgcttcca gtttccccgt tcgctatatg gatgaagaac aacatgtttg
     2821 cgtgacggtc cgaaaccaga tgattcaaaa agtcacctgg tgggatgacc cctccggcct
     2881 accgtttaac cgaatacccg agtcaggtct gccggtttcg gacaatcgag gagcgggagt
     2941 cacggcggac ggcctgtggt tatttgatgg ccctggacaa aaggaattga ccattcgacc
     3001 atcctggcag ctgtccaaac cgaaccattc ggtttcctgg aagccgctcg ccagcacttg
     3061 gcgggacggt tttcccagga aaccccctat gctgactccc caggaagcct tctcttcggt
     3121 tttactgtac cctgataaat ctgaaatgat tggggaaagg gaaagtcaac cttttatttt
     3181 tgattttttg gatgattttc ttgaggaaca ccaggatctc tttcacttac gatctcatgc
     3241 caaacgcgtc ttgctttcac attgcgaagc gggattgggt tcatgccttc agttccagga
     3301 caatcaaatc catatcatct ggtacacctg gccacaattt gctcaaaaac atgcccaatc
     3361 tatttggaat aggctttctc ggatggaccg gttggcgtgg ttttcaaatt ttcaatttat
     3421 cccctttgag cggctcatgt tgaaaacatt gccgaccagg tatgattgga tttatctctg
     3481 gattcccttt tcagattatt caaattccaa tatgatcgac tgttgggtga aatttttacg
     3541 gacctatctt gctgagggga gtgtcggttg tgtggccggt ccccccgtcc tgaaagagaa
     3601 tttccagagg ctaggcattc agattctcta ctctgccgca ggagattcct tgcctccctt
     3661 ccggattcac caaacgatac ttccgaatgg aaggttaaat cctgacttgg cggtctggat
     3721 tattcaaaat cctgttcagt cttagttcca aggagcccta cggcaaaatc tcaaatgtcg
     3781 gttggtcccg ttgaatgaac atgcaccttt ccgatcttga cagccgaacg ttgggcgaat
     3841 agtatgggga aacctttaaa acccatccgg cgaggtgggt aaggagagtc aagcgatggc
     3901 ggccactcaa ttggccattt catgtgtgcc cttctcatgt cgttctgaga agggtttttt
     3961 ttggcttcat ggggtgcgat gacatgaaca cttctcctcg accacacgaa cgacttttaa
     4021 tggatggcca ggaaatggcc aggaccttaa cccgcatgag tcatgagatt ctagagcgga
     4081 ataaaggcat tgaagggtta gcgttggtgg ggatccggac aggcggagtg tttctcgcca
     4141 aaaggattgc cctcagaatt cagcagatcg aaaaacggga cgtgcctctt ggagagttgg
     4201 acatca
//
LOCUS       DATIAL010000350         7285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_65434, whole genome shotgun sequence.
ACCESSION   DATIAL010000350 DATIAL010000000
VERSION     DATIAL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65434"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VLA60_10845"
     CDS             <1..654
                     /locus_tag="VLA60_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HSF09905.1"
                     /translation="NAKDKVLMGVERKSMVLTEEEKRTTAFHEAGHALMAKLLPGTDP
                     VHKVTIIPRGRALGMTMQLPIDDRHSYSKDFLYNTLSILFGGRVAEELIFKSVTTGAG
                     NDIERATDLARKMVCEWGMSEKLGPLTFGKKDEEVFLGRDFGSRRDFSDQVALEIDKE
                     VKRLVVESYERTTRMLTEHIHTLRAIAEALLEKEVLDGIEIEKIVQQSSSLEQAAAV"
     gene            1103..1891
                     /gene="folP"
                     /locus_tag="VLA60_10850"
     CDS             1103..1891
                     /gene="folP"
                     /locus_tag="VLA60_10850"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HSF09906.1"
                     /translation="MGILNVTPDSFSEDGHCLNPETAIERAQQMIAEGADLIDIGGES
                     TRPGASYVDKDEEIRRIAPILTVLRKLTNIPISIDTRKAAVARIALDLGADIINDVSA
                     LQDDPLMAQLIQETGAGVVLMHRQGHSINMQYAPQYKDVVVEIKDFLSERVSMARSMG
                     IPADHIMVDPGIGFGKTLDHNLEILGNIGKFLTLGHPILIGVSRKAFIGTLTGKPVEK
                     REFGNAVAVAAAVWQGAHIVRVHEVGAMHDAIQVAQALQNLCDK"
     gene            2082..3431
                     /gene="glmM"
                     /locus_tag="VLA60_10855"
     CDS             2082..3431
                     /gene="glmM"
                     /locus_tag="VLA60_10855"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HSF09907.1"
                     /translation="MGKLFGTDGVRGVANLDPMTSEMAMKLGRAAAHVFRKREGRHKI
                     IIGKDTRVSGYMLESALTAGLCSMGVDVLLVGPLPTPAIAFMTRSLRADAGVMISASH
                     NPYQDNGIKFFSNDGMKLPDELEHRIENLILSNEIEHIRPTAQAIGKAYRIDDAEGRY
                     IEFVKRSLPREMDFQNMRIVLDCAHGAAYHVFPKVIHELGAKVWVMGNEPDGLNINDG
                     CGAVHPERLQQMVQDRKADLGIALDGDADRALFVCEKGQVVDGDHALAAFALDLKRLG
                     RLRHNTVVGTVMSNFGFEVCMRQAGINLVRTAVGDRYILERMVAEDFNLGGEQSGHMI
                     FLDYHTSGDGMISALQMLKLMKRAQKPLSEIAMCMHSTPQVLVGVAVPRKPDLQSLPQ
                     LQQAIQDKERVLNGTGRILVRYSGTEPLLRVMVEGQDNQVIQKIAEDLITVVKSCIQ"
     gene            3633..6176
                     /locus_tag="VLA60_10860"
     CDS             3633..6176
                     /locus_tag="VLA60_10860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HSF09908.1"
                     /translation="MSYLTGLWFGPFLTFFPLSLLGALFIFGCILTWFEQQRRLTRRT
                     GFILFALVVGGIGHAYWASTTQWDSNLVIEADAQPIFLEGTIVAPVRQTPDGLVLLVE
                     ATHMVKQEGTQMTHGRIRLTWREPGASTLIYGEHIAFMARVREPYGTMNPGGFHYGRH
                     LKRQGIHAVATVQGPEAIRIQGTQERTTRGFILGVVDRWRQSIHHAAVSSLTNPALGL
                     FLGMVLGEQSYIESDIRDAFMASGTVHILSISGSHLGLLALLIFAGARWSLRQLPTVW
                     LERLSLRLTATRFAVLITLPAVSFYTLLAGAEMATVRSWIMIVVCCAGIWLGRERNLV
                     TALGLAALLMVLPHPEAIQDISFQLSYLSVAAIALVAQVRKEKESSLSGIDAAFPPAQ
                     PTWLLGLWHKAVLAWLITLAVSLTTLPLVAHDFHQIPWLGLLANLVIVPLVGAIMIPL
                     GLLSGMIVLVGGGATLPLSSLNQWVFDWLAQVVMMLSHVPGAAWYVASPSMSSMIVFW
                     GMLAVVVAMWHRQLIRWGCGTILLAILLWWGWSPRTGWEPGTVRVTFLDVGQGDATLI
                     ELPDGQTVLIDGGPSYRRLDMGRAVIGPYLWNQGIRRLDHVVATHPQWDHVGGLPWVL
                     DTFEVGHYWDNGISRPERFFSRLHLAVKSAGLQEQTSTAGTVIVSPGPCSLTVLNPSP
                     RQGTEEYGSWTVSSGRDLNNRSLVTRLECGPHSFLFTADAEQQSLNELLQVSNGLSAR
                     IVKVPHHGAKSSLHREWVNRLQAEAMVVSVGAHNRYGHPAPEVLDAYEQRGLPVYRTD
                     RDGAVWFIASLGSDDMTVRTAREGSLLQVQAGPRMWGEEWGNWTRMWNR"
     gene            6440..7168
                     /locus_tag="VLA60_10865"
     CDS             6440..7168
                     /locus_tag="VLA60_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF09909.1"
                     /translation="MANQEQLKLIKAGVNVWNAWRKANPSVRIDLSGGNFSRLELSCV
                     DLAGANLQDVNFNHADLSGAILSGANLTESSLLEANLTGANLENANVSDADLLRVVLI
                     RANLERAHLAKTDLTGADLTQANLTEANLLLANLQGAKLIEANLEGCKLREANLGQAD
                     LASANLGRADLAQANLKQANLGGANVEQASLIQVNLQEANLQDLYNMTLLQLSKARSL
                     RQARLDPPIYTQLAREYPHVCGED"
BASE COUNT         1712 a         1602 c         2016 g         1955 t
ORIGIN      
        1 aacgccaaag ataaggtctt aatgggggtc gaacgtaaga gcatggttct aactgaggaa
       61 gaaaagcgta cgacggcgtt tcatgaggcc ggccacgctt tgatggcgaa actcttacca
      121 ggaacggatc cggttcataa ggtcaccatt atccctcgag gacgggcttt gggcatgacg
      181 atgcaattgc ccatcgatga ccgacatagt tactcgaaag acttcttgta caataccctt
      241 tctattctgt ttggcgggcg cgtggcagag gaattgatct ttaaaagcgt gacgaccggg
      301 gctgggaatg atattgagcg ggctactgat ttggcgagaa aaatggtttg cgaatggggt
      361 atgagtgaaa aattaggtcc gttgacattt ggaaaaaaag acgaagaagt tttcctgggt
      421 cgtgattttg gttctcgtcg agattttagt gatcaagtgg ctttagaaat tgacaaggaa
      481 gtcaagcggc ttgtggttga atcttatgaa cgaacgacca ggatgctaac cgaacacatt
      541 catacattac gggccatcgc tgaagcgttg ttggagaaag aggtgttaga cggcatagaa
      601 attgaaaaaa ttgttcaaca gtcatcttcc cttgagcaag ctgcagcagt gtaacgagat
      661 ttccagattt tttcctcatt cagaatagcc aggtaattct cgttccaggc tgggaaggtt
      721 tattttcgac ccatcccctt tagttatgac ccccccaaaa aaggttgaac accttgtttc
      781 cattgagatc atgtttcttt ttccaccgtg ggtttagagc atattaattg ggcccaccac
      841 tatagtggga gcttcccctt aagaatttat ctaccaaacg ttaccgccca ctattcttat
      901 ttttgtccat tcgcggcttt tcttatcatt caattcgttg aaatttataa ggcggaatgt
      961 cctatttgat ttttaaaacc catcatgacc atgtgttggt taacggtatg agcgcatgaa
     1021 acccgcagtt gtctcagggc caacttttcc tgcaatccga tgccgggaaa ggatgatgcc
     1081 cattgggtca aaggtcttaa tcatgggtat cttgaacgtg accccagatt ctttttctga
     1141 ggatggacat tgtctcaacc ctgagacagc cattgaacga gcacaacaga tgattgccga
     1201 gggagcggat ttgattgata tcggggggga atcgacgagg cctggagcat catatgtgga
     1261 caaggatgag gaaataaggc gcattgctcc cattctgacg gtgttgagaa aactcaccaa
     1321 tatccccatt tctattgata cacgcaaagc tgctgtggct cgcatcgcat tggatctagg
     1381 ggccgatatc attaatgatg tgagcgctct tcaagatgat cctctaatgg ctcaattaat
     1441 tcaagaaacc ggagcggggg tggtcctcat gcaccggcaa gggcatagta taaatatgca
     1501 gtatgcccca caatataaag atgtcgtagt tgaaattaaa gactttttat ctgaacgggt
     1561 ctccatggct cgatcgatgg ggattccggc agatcatatt atggttgatc cgggaatcgg
     1621 gtttgggaaa acccttgatc ataatttaga aattcttgga aacattggga aattccttac
     1681 attgggacac cctattttaa ttggggtgtc acggaaagcc tttataggca cattaacggg
     1741 aaagccggtt gagaaacgag aattcgggaa tgcggttgcg gtagccgctg cggtctggca
     1801 gggagcacat attgtacgag ttcatgaggt gggagccatg cacgatgcta ttcaggtggc
     1861 tcaagcttta caaaatttgt gcgacaagta atagagtcgg tggtaagatc tctccccgcg
     1921 gcgttttcac cgctttgtcc ggcttacgac agctgcttgg accggaattt ttgaattgtc
     1981 tttctcgaca tgggatgcac ttatgagctg ggcgacaaaa tatattgttc gctaatcaat
     2041 ttgttttatg gtcttagttc gttactaaaa aagggaacca catggggaaa ctattcggta
     2101 cggatggggt gcgtggggtc gcgaacctgg atccgatgac tagcgaaatg gccatgaaat
     2161 taggacgggc ggcggcccat gtttttcgta aacgtgaagg acgacataaa atcatcattg
     2221 gcaaagatac tcgtgtgtcc ggatatatgc tggagtcggc cttaactgcc ggactatgct
     2281 ctatgggtgt ggatgtctta ctggtcggac ccttaccgac cccggcaatt gcgttcatga
     2341 cccgtagctt gagagccgat gccggggtga tgatttctgc ttctcacaac ccctatcagg
     2401 ataatggcat aaagtttttt tcgaatgacg ggatgaaatt accggatgaa ctggagcatc
     2461 gtattgagaa tcttattctt tccaatgaaa tagagcatat tcgaccgacc gctcaggcca
     2521 tcgggaaagc ctatcgcatt gatgatgccg aaggacgata tattgagttc gtcaagcgtt
     2581 cgcttcctcg tgaaatggat tttcagaata tgcgaattgt cttggattgt gcccatggag
     2641 cagcgtatca cgttttccca aaggtcattc atgaactagg cgctaaggtc tgggtgatgg
     2701 gcaatgaacc tgacggtctg aatattaatg acgggtgtgg cgcggtgcac ccggaacgat
     2761 tacagcagat ggttcaagac cggaaggctg acctcggtat tgccctggac ggagacgcgg
     2821 atcgggcttt gtttgtctgt gagaagggac aggtggtcga tggagatcat gccctggcgg
     2881 cttttgctct tgaccttaaa cggctaggtc gattacgaca taataccgtg gtcggaaccg
     2941 tgatgagtaa ttttggtttt gaggtctgta tgcgacaggc tggtatcaat ttggtacgaa
     3001 cggctgttgg ggatcgatat atcttggagc gaatggtggc tgaagatttt aatctgggtg
     3061 gagagcagtc cggccatatg atatttttgg attatcatac gagcggtgat ggaatgatct
     3121 ccgccttgca aatgctgaag ctgatgaagc gggctcaaaa acctctgtcg gaaattgcca
     3181 tgtgtatgca ttctactcct caagtgttgg tgggggtcgc cgttccccgc aagcctgacc
     3241 tgcagagcct tccacagtta cagcaggcta ttcaagacaa agagcgggtc ctgaatggaa
     3301 ccggacggat tcttgtacgc tattccggta cggaaccgtt gctgcgagtg atggttgaag
     3361 gtcaagataa ccaggtcatc cagaaaattg cagaagattt aatcacagtg gtgaagtcct
     3421 gcatccagta aggtggtacg gtctgtttct ggctagcttc attaatttgg gttgaatcct
     3481 agattctttt accggcaagg ttcttccgag gcagtggtct accataacct gcattctgtt
     3541 ccgtcgtctg ggagagtggc aaccgctgct gtcggcttgt taaagagttt cattaaccat
     3601 atgcactgtc ctatgtgata ctccttggag tagtgtccta cctcactggc ctgtggttcg
     3661 gcccgtttct aaccttcttc cccctatcac ttttgggggc actttttatt tttgggtgca
     3721 ttctcacctg gtttgaacaa cagcgacgcc taactcgaag gaccggattc atcctgtttg
     3781 cccttgtggt gggaggaatc ggccatgctt attgggcatc gaccacgcaa tgggattcga
     3841 atctcgttat cgaggccgat gcacagccca tcttccttga aggaactatt gttgcaccgg
     3901 tcaggcagac tccggatggg ctagtgttgc tggtagaggc gacccatatg gtgaaacaag
     3961 aagggacgca gatgactcat gggcgaatcc gtctcacttg gcgggaacca ggtgcttcta
     4021 cattaatcta tggtgagcat atcgccttta tggcccgagt acgcgaaccc tatggaacca
     4081 tgaaccctgg cgggtttcat tatggccgac atctaaaacg acaaggaatt catgctgtgg
     4141 cgaccgtgca aggcccggaa gccatcagga ttcagggcac ccaagagcgg actacccgtg
     4201 ggtttatatt gggtgtggtc gatcgctggc gccaatcgat tcatcatgcg gcggtctcca
     4261 gtcttacaaa tcctgcattg ggattgttcc tagggatggt attgggagag caaagttata
     4321 ttgagtcgga tattcgggac gcctttatgg ccagcggaac cgtccatatt ctctcgatct
     4381 ccgggtctca tttggggtta ttggccttgt tgatttttgc cggggcacga tggagcctgc
     4441 gccagttacc gacggtttgg ctggaacgac tttcgttacg actcaccgcc acacggtttg
     4501 cggtccttat cacattgccg gccgtctcct tttatacctt gctggcggga gcggaaatgg
     4561 cgacggtccg ctcatggatc atgatcgtgg tgtgttgtgc cggcatatgg ttgggaaggg
     4621 aacgcaatct ggtgacggcg ttaggcctag ccgcgcttct tatggttttg ccgcatccgg
     4681 aggccattca ggatatttct ttccagttat cctatctctc agttgcggca attgccctcg
     4741 tggcccaggt ccgaaaagaa aaggaatcca gtctatccgg aattgatgca gccttcccac
     4801 cagcacaacc gacatggctc ttgggtctct ggcataaggc tgtgctagcc tggctgataa
     4861 ccctcgcagt cagcttaact actttgcctt tggtcgccca tgactttcat caaataccgt
     4921 ggctgggttt attggctaat ttggtgattg tgcccttggt cggagcaata atgattcctc
     4981 taggtttgtt gtcagggatg atcgtcttag tggggggagg agcaactctt cctttgtcca
     5041 gtctgaacca atgggtcttt gactggctcg cgcaggtcgt gatgatgctc tcacatgttc
     5101 ccggagcggc ctggtatgtt gcatcgccga gcatgagttc gatgatcgtc ttttggggaa
     5161 tgctggccgt ggtcgtggct atgtggcatc ggcaactgat ccgatggggg tgtggtacaa
     5221 tcctgctcgc gattctgctg tggtggggat ggtctccacg cacggggtgg gagccgggaa
     5281 ccgtacgagt cacgttcctg gatgtgggac aaggtgatgc caccctcatt gaattacccg
     5341 acggtcaaac cgtcttgatc gatggtgggc catcctaccg acgattggat atgggacgtg
     5401 cggtcatcgg gccgtatctc tggaatcagg gtatccgaag gctggatcat gtggttgcga
     5461 cgcatccgca atgggatcat gtgggtggat tgccgtgggt gttggatacg tttgaggtgg
     5521 gacactattg ggataatggc atctctcgcc ctgaacggtt tttttctcgt ctccacctgg
     5581 cggtgaaatc tgctggactt caggaacaga caagcacagc aggaactgtc attgtcagcc
     5641 caggaccttg ctctctgact gtgctgaatc cttccccccg ccaagggacg gaggaatatg
     5701 gttcctggac agtgtctagt ggaagggatc tcaataatcg atcgctcgtc accaggctcg
     5761 agtgcgggcc gcattcattt ttgttcacgg ccgatgcgga acagcaatct ctgaatgaat
     5821 tgctgcaggt ttctaatggt ctttctgccc gcatcgtgaa agtaccgcat catggagcga
     5881 aaagttctct gcatcgtgaa tgggtgaatc gtcttcaagc tgaggcgatg gttgtttcgg
     5941 tgggtgccca taaccggtat ggacatccag ctcctgaggt gttagatgcc tatgaacagc
     6001 gaggccttcc tgtatatcgg actgatcgcg atggagcggt ctggttcatc gcaagtctcg
     6061 gatcggacga catgacggtg cgaacagcga gggagggaag tctgctccag gttcaagctg
     6121 gcccgcgtat gtggggagag gaatggggca attggactcg aatgtggaat cgataaaaag
     6181 gtttttcctc taacctgcca tgtggacttc gaggtctttc tcaagtttgg ccttctcggc
     6241 gcattccgaa tccgtcgtgg acgttttaca ataaatttgc cgtagtcgat aactggtgca
     6301 gaggtgggtc tattcccgca tatgaggacg aggggtgtgt caaattttgt atccccgttt
     6361 ttagcgaccg tcgagaacag acgtgtcagc cacaggtaaa atatttatca agatcggtta
     6421 ttgccattaa gggagtctca tggcgaatca agagcaattg aagctcatca aggcgggcgt
     6481 aaatgtctgg aatgcatgga gaaaagccaa tccttccgtt cgcatagatt tgagtggtgg
     6541 aaatttttcc agactggaac tgtcctgtgt cgatttggct ggtgccaact tgcaggatgt
     6601 caatttcaat cacgcggatc tttcaggtgc catattgtcc ggggctaatt tgactgagtc
     6661 cagtttgttg gaagcgaatt tgaccggcgc gaacttggaa aatgcgaatg tgagtgacgc
     6721 tgatcttctc cgggtcgtac tcatacgggc gaatcttgag cgggcacatc tcgccaaaac
     6781 ggatttaaca ggggcagatc tcacacaagc gaatctgact gaagcgaatc ttctcttggc
     6841 caacttgcaa ggcgctaaac tcattgaggc caatttggag ggatgtaagc tgcgagaggc
     6901 aaacctgggc caggcggatc ttgctagcgc caatcttggt cgtgctgatt tggcgcaggc
     6961 caatctcaag caagcgaatc tggggggtgc gaatgttgag caggcttcac tgattcaggt
     7021 gaatttgcag gaggccaatc ttcaggatct ttacaacatg actctcctcc aattgtcaaa
     7081 agccagatcg ttgcggcagg ctcgactaga cccgccaatc tatacacagc ttgcaaggga
     7141 gtatcctcat gtatgcgggg aggattagtt aagaaacttt gtgagatatc aagaaatctt
     7201 tacccgtgtt ggagaattcc caaatgggga caagaaaaat cataccaata gtggaaaacg
     7261 tgattttcga tcctaatctg agaga
//
LOCUS       DATIAL010000351         5289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1247836, whole genome shotgun sequence.
ACCESSION   DATIAL010000351 DATIAL010000000
VERSION     DATIAL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247836"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="VLA60_10870"
     CDS             <1..1696
                     /locus_tag="VLA60_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF09910.1"
                     /translation="KGGFIGLATREAVQKALYHKLATHPIEHIMVRDIFLATPATPFE
                     EVQQHMVERNQRIVPVVDHDQVVGIFSRTDLLRAMHQALPSQGGDVEPSAGAAPSATV
                     VPTPTLRTSNLHKQLASRLPPPLLTLLQTIRTLADTQEVAAFLVGGFVRDFLMNIPNF
                     DLDVVIEGDGIRFGKALAQELKAEWTIHERFGTVSIALPKTLNIPQMHHLDIATARTE
                     YYEYPTALPTVERSSIKKDLYRRDFTINALAIRLNRTPGELLDFFGGRRDLKDRIVRV
                     LHSLSFVEDPTRVFRAIRFEQRFGFQISKETQHFIQQAETMELFQRLSGTRLGNELIH
                     ILEEPEPSKGIQRLSQFKLFPFIHPKLQWKEPAPTLFASGEKILAWHEVESARSGTER
                     WLLYALAWFESLGKAELIKTWKRLGFPQRTTAAVGEFLQVQSTLIRTLNRKHLAPSEI
                     YDLLTPWPKELVLFLMAKAQSKPTIHVAMERIRDYLTTFQHIAITLTGHDLEDMGLPK
                     GPAYRRVLDRIFKAKLDSLVATQEDEYRLAKTFIAQETASGSHSKSSSLARKGNGR"
     gene            1739..3232
                     /locus_tag="VLA60_10875"
     CDS             1739..3232
                     /locus_tag="VLA60_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF09911.1"
                     /translation="MHQLSSPPPPSRIRWRILLLLLLISIITYIDRVNISVTARQMMP
                     ALGLTDLQMGQIFSAFVFGYALFQIPGGWLGDRWGPRRVLTFALIWWSIFTALTAVAP
                     TLPLANLIGIMGSLMVVRFLIGIGEAAALPNFNRAVANWHPPNERGLGIGITIGGIGL
                     GSALTPPLTAWIMVNYGWQTAFYVAGGLGIGIALLWYWYATDHPRQHAHVNTAEAELI
                     EGSESLPDPPAPLEGRSGKAWGMVHDVFTEIETREGRPPASQAKGNGTGGKKDNPNAT
                     TPTRTGRKSETAPVHKSKTTTVPWKAILTTPTVWWLTLSYTCLGYVAYVYMSWFYLYL
                     VNVRGFAILQGAFFASTPFIAMAIFCPLGGWVTDRLTEQYGINRGRASVGGAGMILAA
                     LSIIIGANIEAPYVAIGFLSLGAGWLYFTVGPFWSSTTDLSKPYAGTLSGLMNTGANL
                     GGTLSPTLTPWLADTFGWSVSLGTAALVAFIGGLCWIRIRPGMGIRS"
     gene            3587..3844
                     /locus_tag="VLA60_10880"
     CDS             3587..3844
                     /locus_tag="VLA60_10880"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HSF09912.1"
                     /translation="MVTNEPRISTIEVSDETITAQLTDGRMISVPLVWSWRLTNATPE
                     QRKNFQIIGNGHGVHWPDVDEDISAIGMLEGLPARPVKHPV"
     gene            4291..>5289
                     /locus_tag="VLA60_10885"
     CDS             4291..>5289
                     /locus_tag="VLA60_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF09913.1"
                     /translation="MALSIFLLFPGVVCATEIEEFKKGVVKITALVNGLPSVGTGFIV
                     RLDKDGAYIVTAAHVISGDSKPQVWFFPHPHQSFASQVLGIDGTNENGLATLRVPDPI
                     PDGLVALPLDQTTQASGGETITFIGFPPNLAPWTVSTGSISGRKGPHLMFQALVEEGH
                     SGGPLLLGGKVIGVVTDAQGRLGYAVPTPVLAIALKGWHVEPSGSDSLSREISGADGA
                     PMVRIPAGQFETNVESRSGTGDISEGSRYTVYVEDFFIDKYEVTIERYGKFLQVTGRT
                     APEYWDHIMMPRDANRPVVGVTWHEAKAYCHWARKRLPTEDEWEKSARGTDARIYPW"
BASE COUNT         1255 a         1524 c         1335 g         1175 t
ORIGIN      
        1 caaaggcggc ttcataggat tagccacacg ggaagcggta caaaaagccc tctatcataa
       61 actggccacc caccccatcg agcacatcat ggtgagggat atctttctgg ccacacccgc
      121 cacaccgttt gaggaggtcc aacagcatat ggtggagcgg aatcagcgga tcgtcccggt
      181 ggtggatcac gaccaggtcg ttggcatatt cagccgaacc gatttacttc gggcgatgca
      241 ccaggcactc ccgtcccagg gaggagacgt cgaaccctct gccggagcag ccccttcagc
      301 taccgtcgtt cccacaccaa ccttgcgtac ttcaaatctt cacaaacaat tggccagccg
      361 tttacctccg ccgctcctaa ccctcctcca gaccattcgg acactcgccg acacccagga
      421 ggtggccgcg ttcctcgtcg gcggattcgt ccgggatttc ctgatgaaca ttcccaattt
      481 cgatctggac gtggtgatcg aaggagacgg cattcgtttt ggcaaagcgt tagcgcagga
      541 actgaaggcc gaatggacca ttcatgagcg atttggaacc gtctcgatcg cactccccaa
      601 gactctcaat attccacaga tgcatcacct ggacattgcc acggctcgaa cggaatacta
      661 tgaatacccc accgcactac cgaccgtgga acgcagttca ataaaaaaag acctctaccg
      721 gcgggatttc accatcaatg ccctggccat tcgcttaaac aggacgcccg gagaactcct
      781 ggactttttc ggaggccggc gggacctcaa ggatagaatc gttcgggtcc tacacagcct
      841 cagcttcgtg gaagatccca cccgggtctt tcgggccatt cgcttcgaac agcggttcgg
      901 tttccagatc agtaaggaaa cgcagcattt cattcaacag gccgaaacca tggaactgtt
      961 tcagcggctc tccggcacac gattagggaa tgaactgatc catattctag aggaacccga
     1021 gccgtccaaa ggcatccagc gattgagtca atttaaactc ttccccttca ttcatcccaa
     1081 gctgcaatgg aaggaaccgg ctccgacgct ctttgcatcc ggagaaaaaa tcctggcctg
     1141 gcatgaggtg gaaagcgcca gatcaggaac cgagcgatgg ctgctctatg ccctggcctg
     1201 gttcgaatca ttgggcaaag ccgaattgat caaaacctgg aaacgattgg gattcccgca
     1261 acgaaccaca gcagccgtcg gagagttctt acaggtccag tccacgttga tacggaccct
     1321 caacagaaag cacctggctc cttcagaaat ttacgatctc ctcacgccat ggccgaagga
     1381 actcgtgttg ttcctcatgg ccaaggcaca aagcaagccg acgattcatg tggccatgga
     1441 acggatccgg gattatctca ccacgttcca acatatcgcc atcaccttaa ccggccacga
     1501 cctggaggac atgggccttc ccaaaggtcc ggcgtatcgc cgggtgctcg atcgcatttt
     1561 caaagccaaa ctggatagcc tggtggccac acaggaagat gaatatcggc ttgccaaaac
     1621 cttcattgca caagaaaccg cttcaggaag ccactccaaa tcctcttcat tggcaaggaa
     1681 aggcaacggg aggtagcctt cccgtcggct cccccggcaa gtagtctatt cattccccat
     1741 gcaccagcta tcatcgcccc ccccaccgtc tcgaatccgc tggcgaattc ttctgctcct
     1801 cctcctcatc agcatcatca cttacatcga tcgggtgaat atttccgtca ccgcccgcca
     1861 aatgatgccg gcactcggtc ttaccgatct gcaaatgggt cagatctttt ccgcctttgt
     1921 ctttgggtac gccctctttc aaattcccgg tggctggttg ggcgatcgct ggggaccacg
     1981 tcgcgtattg accttcgccc tcatctggtg gtcgatcttc acagcgctga cggccgtcgc
     2041 tcccacgctc ccactggcca acctgatcgg gattatgggt tcactcatgg tcgtgcgttt
     2101 cctcatcggc atcggcgagg ccgccgcgct gcccaatttc aatcgagccg tcgcaaactg
     2161 gcatcccccg aatgaacggg gcttaggcat tggcatcacc atcggaggga tcggcctggg
     2221 ctcagccctc acaccacctc tcaccgcctg gatcatggtc aattatggct ggcaaacggc
     2281 gttttatgtc gcaggcggat taggtatcgg tatcgcactc ttgtggtact ggtacgcaac
     2341 cgatcatccc cggcaacatg cacatgtcaa caccgccgag gcggaactga tcgaaggttc
     2401 ggaatctcta cccgaccctc ctgctccctt ggaaggacga tccggcaagg cctggggaat
     2461 ggtccatgat gtgttcaccg aaatcgaaac acgcgaagga cggcctccag cctcacaggc
     2521 aaaaggcaac ggaaccggtg ggaagaagga taatcccaac gccaccaccc caacacggac
     2581 agggaggaaa agcgaaacag cacccgttca caaatcaaag acgaccaccg ttccctggaa
     2641 agccatcctc accacaccca ccgtctggtg gctcacgctc agttacacct gtctcggcta
     2701 cgtcgcctat gtctatatgt cctggtttta tctttatctc gtcaatgtga ggggttttgc
     2761 catccttcaa ggtgctttct tcgcctcgac gccgtttatc gcgatggcca tcttctgccc
     2821 attgggagga tgggtgaccg atcgtctcac cgaacaatac gggatcaatc gtggccgcgc
     2881 gagcgtggga ggagcaggaa tgattctggc cgcgctctcc atcatcatcg gcgccaacat
     2941 cgaagcgcct tatgttgcga tcggcttcct ctcgcttggt gccggatggc tgtatttcac
     3001 cgtcggcccg ttctggtctt cgaccacgga cttatcaaaa ccctatgccg gcacgctctc
     3061 aggcctcatg aacaccggcg ccaacctggg cggaaccctc tcccccacac tgacgccgtg
     3121 gctggcggac accttcggct ggtccgtctc tctcggcacc gccgccttag ttgcgtttat
     3181 tggcggattg tgctggatcc gcattcgtcc gggaatggga ataagatctt aataccagcc
     3241 agacgtcagg tgccttgcta taaccactta gtacttctct cgccacctgg agtctcattg
     3301 ccgtttttcg tatactgtca gtcaacaaca gttcgataag gaaatttgga aagtatcgtg
     3361 ccaactgttc gagggattga aggaccttac cgatttttct tttatagttt tgactgcaac
     3421 gaacccaaac atgttcacgt ccaacgagaa cgtcatatct gcaaattttg gctagaaccc
     3481 atttccttgg caggtaatga tggatttgcg ccacatgagt taaatcgaat ccgaaaaatc
     3541 ctgctcgacc agagagacaa aatgatcgaa gcctgggagg aacattgtgg tcacgaatga
     3601 acccagaata tccacgatag aggtctcaga tgagaccatc accgcgcagt taaccgacgg
     3661 acgaatgatc agtgtgccct tggtatggtc atggcggtta accaatgcta ctcctgaaca
     3721 acgcaagaac ttccaaatta ttggaaatgg acatggagtg cactggcctg atgtggatga
     3781 ggacattagc gctatcggaa tgctcgaagg ccttcccgca cgacctgtca aacatcccgt
     3841 ctaacccttg cgattcaacc catccctatc ctcaaacatc aatttcattg tagatctttt
     3901 ctgtcggctg gtccgtacac cctcggcatc gccgcccgcg ttgcattgct cggtggactc
     3961 tgttgggtct tcattcaacc aggacatggg ctcaatctcc ccaggtcagg cgatcgagcg
     4021 tgaacaagtc tgaacggttc acaatacaga cgagccccaa aatgattcta ttatttctgg
     4081 atcatgataa actgagtcgc tgaactggaa tataaaaaac agccagtcac aaaatcgtga
     4141 tggggccggg cactcccgat tgcggagaaa aatacggtga tcccaaaagt tgttcaaaca
     4201 atcagaaccc acaagaacca ccgaacattg ccatcctcca atagggtgcg gggccattca
     4261 tggatatggg tgccttttat cggctgcatc atggccctct ctatcttcct gctctttccc
     4321 ggtgttgttt gtgccacgga gattgaagag ttcaaaaagg gcgtcgtcaa aattacggcg
     4381 cttgtaaatg ggctaccaag tgtcgggacg ggatttatcg tgaggctcga caaggacggc
     4441 gcctatatcg tgacggccgc acatgtgatt tctggagata gcaagccaca ggtgtggttc
     4501 tttcctcatc ctcatcaatc gtttgcctca caggtgttag ggatagatgg aaccaatgaa
     4561 aatgggttag ccacgttgcg tgttccagat cccattcctg acggactcgt ggcgcttccg
     4621 ctcgatcaga ccacccaagc atccggggga gaaaccatta ccttcatcgg ttttccaccg
     4681 aatctcgctc cctggacagt ctcaaccgga agtatcagcg ggaggaaagg gccacacctg
     4741 atgtttcagg cgttagtgga ggaaggtcat tcgggtggcc cccttctgct tggtggcaaa
     4801 gtgatcggag tcgtgacaga tgctcaggga cgtctggggt atgccgtccc cacaccggtc
     4861 ttggccatcg cgttgaaagg atggcacgtg gaaccatcgg ggagcgactc gttatcacga
     4921 gagatttcag gcgcagacgg tgcaccgatg gttcggattc ctgcggggca gtttgaaacg
     4981 aatgtagaat cacgctcagg aacgggggac atttccgaag ggagtcgata taccgtctat
     5041 gtcgaagatt tctttatcga caagtatgaa gtcaccattg aacggtatgg gaaatttcta
     5101 caggtcacag gtcgaacagc cccggaatat tgggatcaca tcatgatgcc ccgggatgcc
     5161 aatcgccccg tcgtcggtgt gacatggcac gaggccaaag cctactgcca ttgggccagg
     5221 aagcgattac cgaccgaaga tgaatgggag aaaagcgcac gaggaaccga cgcacgcatt
     5281 tatccatgg
//
LOCUS       DATIAL010000352         9134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1140123, whole genome shotgun sequence.
ACCESSION   DATIAL010000352 DATIAL010000000
VERSION     DATIAL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140123"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..663)
                     /locus_tag="VLA60_10890"
     CDS             complement(97..663)
                     /locus_tag="VLA60_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HSF09914.1"
                     /translation="MSQETEELWSRRFFLKSSLAGMLILGSRLMFPESASAQSLPEGR
                     LHLYNAHTDERLQVTYRNQSGRYDQEALKDINYLLRCHHSKKICQMDIQLLEYMNQVE
                     KLVGQGKEIHVYSAYRSPSYNKLLIRLGRGAAPNSLHTVGQAMDFSIPGVRLSKIRRA
                     AVKLRLGGVGYYGRRGFIHIDTGSVRYW"
     gene            1680..3257
                     /locus_tag="VLA60_10895"
     CDS             1680..3257
                     /locus_tag="VLA60_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09915.1"
                     /translation="MPQVEPSVMLNSIMGKIYNVLTNGDDTVPKSEDNFFSWCTPGLA
                     VEPDDFDFLTQGLTGVVKKKALKDMVLEGEGGESNSPELTPAVLEGLRAQDASKQYMN
                     AESFARLVDFVPDLAATTNNQFAAMSIMNNEGSLSERFEYILRMSQIMETKLDEKTKG
                     KIEKFRGLLSTTRVKKDLLTDEEIEVSEPSALVNLYNQKMTEYEAAALAYNSARIDAL
                     TADNVKAVHYWGMNANILRNRVKAAMGDWVSNGYKNDYEGINAFIDQVMRRDMALLKA
                     QYQDDLEKARITGMSSGSDFFYTSLVPSNPMQAAGWTNFSFQSSDYNNSSNSSYQMKR
                     SKTSAGGGWLGIFGGGGSASNASGQSESKVTFDSNQFYMNFSITQCQIVRPWFKTSFI
                     NSKLWRFDQNNPESSKQIVSDGGKPATGLCPAYPTAIIFIKDLVIGFRNSSGFSEAAQ
                     SWERSSASGGGAVHFGPFHFGGSHGRSSASGERSSKFHYDSEKQEMRVPGHQIIGFKC
                     HVFPKSPDPLSEITDWI"
     gene            3354..4916
                     /locus_tag="VLA60_10900"
     CDS             3354..4916
                     /locus_tag="VLA60_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09916.1"
                     /translation="MAIDSIVQLALMSKAKKVFEKEGTFLSFPVTPLAYTKEDLDFFS
                     QDTGSDLLRSKANLAAFSTLVNLIPHDEAWLPTDARFLWNELEYVLRESTLAASTRTP
                     EEEVAYQEALSFLKVPGEGGLLQDSPKVRVYNQYKDAYIMAEQEFMEARSTGEAAEDE
                     SEKTQWREVDEPAFRAKLKDLDNQWILEGYKNEVNIARSQYQNLGAKSPTITWEEWKG
                     SFNQDLDSETTAQDNFTVFPSSFTPSNALEEGSWNPFSLSEAEINILVNEAPADLRER
                     FSADGKVSTMKSVTLEFSSAAIKRSWFDSEIFRSRFWRLSDSTKILSDGGSPPSGDCP
                     AYVTAIVFARKVVVEAKQTQSTSSNPKMSVDLRFNYAVNDQNSIKRINPAVLEAVQPQ
                     LMKRPPIPPQQIKTQPVQPLIMKMRPQAMASFKSTAIRVPTQSTRGNVLLKPAVTTPM
                     VARPAYSRSMVTLQASTYIRLPTTPVRKPAPRPATPASPPPDVADKNIYILAFICKPL
                     PKCPDPDLALRW"
     gene            complement(5141..5419)
                     /locus_tag="VLA60_10905"
     CDS             complement(5141..5419)
                     /locus_tag="VLA60_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09917.1"
                     /translation="MNDRIDVKMTMTKSIHMLSMQQAINAATKMLDGFFNSTHCYPES
                     QENHSSCRMTLVDHAIQGTNVCVEIKSEEIIHATPHPVSATPPADIQQ"
     gene            complement(5895..6647)
                     /locus_tag="VLA60_10910"
     CDS             complement(5895..6647)
                     /locus_tag="VLA60_10910"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSF09918.1"
                     /translation="MARTLQQVPLPPTSLDIRIVTYNIHRCCGMDRRIMPRRIAEVLS
                     DLHPDVIALQEVLGLGPKGRGQDELLGALMGMGWVMASARLRHGYPFGNMILSRFPIV
                     NDAQHDLTWRTRRGRCCQRADLRLGNQTLHVYNVHMGTGLRERKYQAERLAILLDQHQ
                     GAGPKIILGDFNEWGRGVTTDILTPRFQSLDIRPFVKRRRTYPGFLPLLHLDHIFYEG
                     RIDVNHVHLPKSRAALIASDHLPLVADLRIRF"
     gene            complement(7141..8400)
                     /locus_tag="VLA60_10915"
     CDS             complement(7141..8400)
                     /locus_tag="VLA60_10915"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HSF09919.1"
                     /translation="MRILILNSGSSSLKFRLADSPSSSVGTDTDSTTLLQGIVTGIGK
                     VATLKISAPEHAAFTSTRQNILDHGQAVGWVWESLQGLMTPDGETLRGPGITIDAVGV
                     RVVHGGHRFTKSVLIDETVLGQIEELSILAPLHNPPCLEGIYQVRALLGPQIPIVAVF
                     DTSFHRTLPDHARTYAIPRDWTLQHGIERFGFHGIAHASLAHSYARYSGRSLEGTRLI
                     TFQLGQGCSVSAIKNGAVVDTSMGFTPLEGLVMGTRCGDMDPAIIGYIAQHAPHSFEE
                     IDHQLNTQSGLLGLSGQTSDMRQLLKAVHERQDPHATLAIEVFCHRARKYLGAYLATL
                     GGADAVVFGGGIGERAPDIRSRICQGMDWCGLSLDCPRNFEASDVQAGEAIPIHSKDS
                     AIKLLVIGTDEESWVARETFQCLRNAR"
     gene            complement(8513..>9134)
                     /locus_tag="VLA60_10920"
     CDS             complement(8513..>9134)
                     /locus_tag="VLA60_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HSF09920.1"
                     /translation="DCCNDKACEIEALQDRQSSILKIVLAINAVMFLIELTAGLLAGS
                     VSLIADSLDMLGDALVYGFSIYVVARGARMKAKAAFLKGGIMAAFGLFVFGQAVYKMV
                     FPQVPVFEAIGAIGLLALAANSLCLLLLWRHRADDINMSSVWLCSRNDIIANASVLCA
                     AVGVWLTTSGWPDILVGLGLAALFLRSALFVLRGAQTELRAVPTVN"
BASE COUNT         2436 a         2105 c         2348 g         2245 t
ORIGIN      
        1 tgcaagcagg atttattttc ccaattaaag gactattcct catagccctc acaagaccgg
       61 atgatattgg ttggaatgtg ataatgaaag aaggaattac cagtaccgga ccgatccggt
      121 atcgatatgg atgaatcctc gccggccgta atagccgact ccacccaacc gaagcttcac
      181 cgcggcgcgt cgaatttttg aaagtcgaac gccgggaatc gagaagtcca tcgcttgtcc
      241 gaccgtatgt aggctgtttg gcgcagcccc tcgaccaagg cgaatcaaca atttattgta
      301 tgagggcgac cggtaggcag agtagacgtg aatttccttt ccctgtccga ctaacttttc
      361 tacctgattc atatattcca gtaattggat gtccatctgg cagatttttt tggaatgatg
      421 gcatcgcagg agataattaa tgtcttttag ggcttcctgg tcatagcggc cggattgatt
      481 ccgatacgtc acctggagtc gttcgtcagt gtgggcgttg tagaggtgaa gccggccttc
      541 aggcagggac tgggccgatg cggattccgg aaacatcaac cggctgccaa ggatcagcat
      601 gcctgcaagt gaggacttca gaaagaagcg cctggaccag agttcttctg tctcctgaga
      661 catggtgaca ccctccgaac tatgaatggg gaaatgtagc gggagaataa agcggcatga
      721 aacactgatt gtttaacaaa ataggatgat gaggtcaacc aatttatgga aaattttcaa
      781 tcttcccctt atccatttgt attgagtcca taacctggtt gcatgaaaat ggccaatcac
      841 ctcctctttt ggccccattt tttttcgcgg aaactcacct gcattgctgt tctttccgac
      901 attctgcatt tgggaatcaa tcctcagcgg cctcatgtcc gacatgttga atccgacatc
      961 gatcattgtc gaattttgtt ttgattcccc ttacagacct aggggaatcc tggcagtatg
     1021 ggatatcgct tttccgcata tcaaaaaggg tagacgatgg gtgatctcac cgtttccttt
     1081 tttctttaac ctatagagtt ccggccgatg gagatattgt taaccaagat atccgattgg
     1141 catttgcctc tctctcctgg cgtcattctc ctagttcgtt acagcccgag tcagtgggca
     1201 taagccttca gggctcattg gcctgtccac gaatcccgtt ttggaagggt atttccagaa
     1261 cgtcccacct gtttccagat tgtcctgatt ttcgaagtct cccacgtttt actggtgaat
     1321 cattcctttc ataattcttc gtttggcttc gtgagtggca ggaatcggat gtttaatctt
     1381 gactccaatc caaactttca acaagccagg aagtgggttc cggttatcca aatagataaa
     1441 catgcctcac aattggatac accgtgaacg ttatgaaagg aacgcctgcg tcaagtgagc
     1501 actgaagaga aggttgaaaa gagttgaaat acaaagggga tgggtctgca caacacttgt
     1561 caacatacca catgaaaatt gggaataagt cttgctcacg tgaataaaac atatgggccc
     1621 aattatttaa tttccacgta tcctgtaaag ggtacgtctt actggaagga ggagtcgtca
     1681 tgccacaggt tgaaccgagc gttatgctta attcgattat ggggaaaatc tacaatgtgc
     1741 tgaccaatgg ggatgatacc gtccccaaat cggaagacaa tttttttagc tggtgcacac
     1801 ccggattggc tgttgaacct gatgattttg attttttaac acaagggtta acgggtgtgg
     1861 tgaaaaagaa agccctgaag gacatggtgc ttgaaggtga gggaggggaa tcgaattcac
     1921 cagagctgac accggcagta ttagaaggat tgcgtgcgca agatgccagc aaacagtata
     1981 tgaatgcaga aagttttgcg cggctcgtcg attttgttcc cgacctggct gccaccacga
     2041 ataatcaatt cgcggctatg agtatcatga acaatgaagg atcgctttca gagcgcttcg
     2101 agtatatttt acggatgagt caaatcatgg agaccaaatt ggatgaaaaa accaaaggaa
     2161 aaattgaaaa attcagaggg ctgctttcca cgacccgggt taaaaaggat cttctgaccg
     2221 atgaagagat cgaggtttca gagcctagtg ccttagtgaa tctgtataac cagaagatga
     2281 cggaatatga agcggcggca ttggcataca acagtgcccg catcgatgcc ctgaccgccg
     2341 acaatgtcaa agcggtccat tattggggga tgaacgccaa cattctgcgc aatagggtca
     2401 aggcagctat gggagattgg gtcagtaatg ggtataaaaa tgattatgaa gggattaacg
     2461 cgtttattga ccaggtcatg agacgggata tggcactgct gaaagcccaa tatcaagatg
     2521 atttggagaa ggccagaatc accgggatgt catccggcag cgattttttc tatacctcgc
     2581 tcgtgcctag caaccccatg caggcggcag gatggaccaa ctttagtttt caatcttcgg
     2641 attataataa ttcctccaat tccagttacc aaatgaaacg gtctaagaca agtgccggag
     2701 ggggctggtt aggcattttc ggcggtggcg ggtctgcgag caatgctagt gggcaatcgg
     2761 aatcgaaagt tacgtttgac agcaaccaat tttatatgaa tttttctatt acccaatgcc
     2821 aaattgttcg gccttggttt aagacaagtt ttatcaatag taagttgtgg cggtttgacc
     2881 aaaataatcc cgaatcatca aagcaaatag tcagtgacgg cggcaaacct gccacaggac
     2941 tctgcccagc ctatcccacg gcaatcattt tcatcaaaga tctcgtgatt ggctttcgaa
     3001 atagcagtgg attttcagag gcagcacaaa gttgggaacg atcgtcggcg agtggtggtg
     3061 gcgccgtgca ttttgggcca tttcatttcg gtggctcgca tggccgtagt tctgccagtg
     3121 gagaacgttc ctcaaaattc cactacgatt cggaaaaaca agaaatgaga gtgccgggtc
     3181 atcaaattat tggattcaaa tgccatgtgt tcccaaagag ccctgatccg ttaagcgaaa
     3241 tcactgattg gatttaacgc tcgaaggagc cccgcctcaa aatgaaaagt tggggcgggg
     3301 ctaccagggg aatgggggaa ttcgaccaac ctaactactt gatgaggtaa gacatggcta
     3361 tcgactccat cgtgcaattg gccttgatgt ctaaggccaa aaaggtcttt gaaaaggaag
     3421 gaacgtttct gagttttcct gtgacaccgt tagcctatac aaaagaggat ctggatttct
     3481 tttcacagga taccgggagt gatcttctca ggagcaaggc caatctggcc gcgttttcaa
     3541 cgctcgtgaa tctgattcct catgatgagg cctggttacc cactgatgca cgctttttgt
     3601 ggaatgagtt ggaatatgtt ctgagagaaa gcacgcttgc tgcttcgact cgtacaccgg
     3661 aggaagaagt ggcgtatcag gaggcgttga gttttttgaa agtccccggt gagggaggcc
     3721 ttttgcagga cagcccgaag gtcagggtct ataaccagta taaggatgcc tatattatgg
     3781 ctgagcagga gttcatggag gcgagaagta cgggtgaggc tgcggaggac gaatccgaga
     3841 aaacacaatg gcgggaggtc gatgagcctg cgtttcgcgc aaaactcaag gatctggata
     3901 atcagtggat cctggagggt tacaagaatg aagtcaatat tgccagaagt cagtaccaga
     3961 atcttggagc caaatctccc acaataacct gggaggagtg gaagggatct ttcaatcagg
     4021 atctcgatag tgagaccact gcgcaggata atttcacggt tttcccaagc agctttaccc
     4081 cgtcaaatgc cttggaggaa ggttcgtgga atcctttctc tctgagcgag gcggaaatca
     4141 acatcttggt gaatgaagcg ccggcagatc tccgcgagcg attctcggct gatggaaagg
     4201 tgtctacgat gaaatctgtg actttggaat tcagttctgc ggcgatcaag cggtcctggt
     4261 ttgattctga aatattccgg tcacggttct ggcggttgtc agattcgact aagattttat
     4321 ctgacggtgg cagcccgcct tccggagatt gtccggccta tgtcacggca atcgtctttg
     4381 cccggaaggt ggtcgtcgag gcaaaacaaa cccaatcaac ctcttccaac ccgaaaatgt
     4441 ctgtagattt gcgttttaac tatgcggtga acgatcaaaa ttcgataaaa aggataaacc
     4501 ctgctgtcct ggaagccgtg caaccgcaat tgatgaaaag gccaccgata ccaccacaac
     4561 aaatcaaaac ccaacctgta caacccttaa tcatgaaaat gcggccccag gcgatggcct
     4621 cgtttaagtc aacggccatc agggtgccaa ctcagtcaac cagagggaat gtcttgttaa
     4681 aacctgcggt tacgactcca atggtcgctc gcccggctta ctccaggtcc atggtgaccc
     4741 tgcaggcgtc gacctatatc cggttgccga cgacgccggt gcggaagccg gcgccacgtc
     4801 ctgcaacacc ggcatcgccc ccgcccgacg ttgccgacaa gaacatttat atattggcgt
     4861 tcatttgtaa gccattaccg aaatgccccg atcctgattt ggccttacgg tggtgattcc
     4921 gaattgaaga agaatggtaa agccaaatgt catgaaagag aaggtcttga tcatgcaggg
     4981 atatgcattt tatctgtaaa taatattagg cctagcaaat gattgagaaa agaaatacct
     5041 ggtgaatcta tgggcaggtg caagggggtg ttcagtcaat ataaatggtg agctttaccg
     5101 aatagatgaa gaggcaccag attttccggt ccggccttcc ctattgctga atgtcggcag
     5161 ggggcgtggc tgatacggga tgaggagtgg cgtgaataat ctcctcagat ttgatttcga
     5221 cacagacgtt ggtgccttga atggcatgat cgacgagagt cattcgacag gacgaatggt
     5281 tctcctggct ttccggataa caatgagtcg aattgaagaa tccgtctagc atttttgttg
     5341 cggcgttaat tgcctgctgc atggaaagca tgtgaatgga tttggtcatg gtcattttca
     5401 catcaatccg gtcgttcaaa atcctatgct tccgatttcc ctactaggtg gtatgtaaaa
     5461 agcataaggc gtgccaatga catacgacat tatgcactcg tgggacatgc cacagcatga
     5521 attgattcaa atgggaattt tcctgaaatc tcatgacgat cttttgtctt agaaatctcc
     5581 tgcttccttc gatttcagac aaatgaaggc cttgattctg gagtaaaaga caaattgatc
     5641 caaaaacccg acaatttttc ttatcttcga tttgactgtg ttgtttcaac aacatcgata
     5701 gtaatgtgat ctattccaga agagttttat aaggaattga ggcaggtgtt tcccgaaacg
     5761 gaaaccgatg gaaatttgtg tgaagggttt atctggtaaa aagaaaaagg ggtgagatcc
     5821 aagagaaatc ggaatgagga cctccgaacg acaatctgaa cggattgccc ggtgctctta
     5881 aggaagattg cacgttaaaa gcgtatgcga agatcggcga cgaggggaag gtgatctgag
     5941 gcgatcagtg cagcacgaga tttcggaaga tgcacatggt ttacgtcaat ccggccctcg
     6001 taaaaaatat ggtcgagatg caagaggggc aaaaatcccg gataggttcg gcgtcgtttg
     6061 acaaatgggc gaatgtccag gctctgaaat cgtggggtca ggatatcagt ggtcacacca
     6121 cgcccccact cgttgaaatc cccgagaatg attttcggac cggctccttg atgctggtcc
     6181 agtaggatag ccaggcgctc cgcttgatac ttgcgttccc gcaaaccggt ccccatatgc
     6241 acgttgtaga catgtaaggt ctgattgccc agccggagat ccgcgcgttg acaacatcgg
     6301 ccgcgccggg ttctccaggt caggtcatgt tgcgcatcat tgacaatcgg gaatcggctc
     6361 aaaatcatgt ttccaaaagg atatccgtga cgcagcctgg cagatgccat tacccatccc
     6421 atacccatca acgcaccaag cagttcatcc tgtccacggc ccttggggcc aagccccagg
     6481 acttcctgta gggcaatgac atccggatgc aggtccgaca gaacttccgc aatccgtctg
     6541 ggcatgattc gacgatccat cccgcaacac cggtgaatgt tataggtcac gatccgaatg
     6601 tcgagggaag tcggcggtag gggaacctgt tgaagtgtcc gggccatagg tgaatgaaaa
     6661 atctttttaa tcagggagcg gcgattgcta tagagaccat atcatgccgg gataatgatc
     6721 tgaggcataa aaaaatgtcc cattatttta attggcaaat tatagaccag gagagaaaaa
     6781 tgccgtccac aaattttgtg gataacttgg tggttaatgg caaaaacagt cctctttctg
     6841 aaggattgat aaagaaatga gcggaatgca catattttag gcattgtatg aaaattattt
     6901 ttgggtacaa catatagggg tgataaattt ttggcgtagt cgtaaaggta gatggcagat
     6961 tatatcttga cttttttgat ttagttgatc ctcgtgagtg tcttcaaact cttatcatga
     7021 tcatctaaaa atactttatc cacagaatgg attttgcctt gttgataatc aactaaatga
     7081 gaatgatttt gtcagctcct atagggcatt ttcatctggc caatacgttt tcgcttgtgt
     7141 tcatcgggca tttctcaggc attgaaatgt ttcacgggca acccacgact cttcgtcggt
     7201 tccaatcacc agcaatttaa tagcggaatc cttgctatga atgggaatgg cctctcctgc
     7261 ctgcacatca gaggcctcga aatttcgagg gcaatccagt gagagaccgc accagtccat
     7321 tccctggcag attcgagagc ggatgtcagg agcccgttca ccaataccgc ccccaaagac
     7381 gaccgcatcg gctccaccca aggtagccag ataggctccc aaatattttc gtgcccgatg
     7441 acagaacact tcaatggcca gagtcgcgtg aggatcctgt cgttcgtgga cggccttcaa
     7501 gagttgacgc atatcggagg tctggccgga caagcccaac aggccggatt gtgtattcaa
     7561 ttgatggtcg atctcttcaa acgaatgcgg agcatgttgg gcaatatacc cgataatggc
     7621 cggatccata tctccacatc gtgtgcccat caccaggcct tctaacggtg tgaatcccat
     7681 tgacgtgtca acgacggctc cgttcttaat ggccgaaacc gagcagcctt gccccaattg
     7741 aaacgtgatg agacgggttc cctccaacga tcgaccggaa tagcgggcat agctgtgggc
     7801 cagagacgca tgggcaattc catggaagcc aaagcgctca atgccatgct gtagcgtcca
     7861 atcccgggga atggcatagg tgcgggcatg gtcgggaagc gtgcgatgaa agctggtatc
     7921 gaataccgcc acgatgggta tctgtggacc caatagcgca cgaacctgat agatgccttc
     7981 caggcagggt ggattatgga gcggagccaa gatgctcaat tcttcaattt ggcccaagac
     8041 ggtctcatcg atcaggacgg attttgtaaa ccggtggccg ccatgaacga cccggacgcc
     8101 tactgcatca atggtgatcc cgggtcctct taaggtttct ccgtctggtg tcatcaggcc
     8161 ttggaggctt tcccataccc aacccactgc ctgcccatgg tccaggatat tttgccgggt
     8221 tgaggtaaac gcggcgtgct cgggagcgct gatcttcagg gtcgccactt ttcctattcc
     8281 ggtaacgatg ccctgcagca aagttgtgga atcggtatct gtcccgactg atgaagatgg
     8341 agagtcggcc aggcggaact ttagagaaga actgccgctg tttaaaatga gaatgcgcat
     8401 tgaaggggct accgaatgct tgctggcatg agcgggatat accctagcac aggcggggaa
     8461 acaggagaag ggagggtggt ttagccagag ctgttgaccc tcagcaggaa agttaattta
     8521 cggtagggac cgctcgaagc tccgtttgag ccccgcgcaa gacaaaaagg gctgaccgta
     8581 gaaaaagagc cgcgagtccc aagcccacga gaatgtcagg ccaacctgaa gtggtgagcc
     8641 acaccccaac ggcggcacac agtaccgagg cattcgcaat aatatcgttg cgggaacaca
     8701 accacaccga actcatattg atgtcatcgg cccgatgccg ccaaagtaag agaaggcaaa
     8761 ggctattcgc tgcaagcgca aggagaccaa tagcgccaat ggcctcgaat acagggacct
     8821 gtggaaacac catcttataa accgcctgcc caaacacgaa tagtccgaag gccgccatga
     8881 tcccgccttt gaggaaggcg gccttggcct tcattcttgc gcctcgcgcc acgacgtaaa
     8941 tgctgaagcc atacaccagc gcatcaccca gcatatccag cgagtcagcg atcaaggaga
     9001 cagaaccagc gagcaaccct gcggtgagtt ctatcaggaa cattaccgca ttaatcgcca
     9061 acactatttt gagtatagag ctttggcgat cttgaagcgc ctcgatctca cacgctttgt
     9121 cattgcaaca atca
//
LOCUS       DATIAL010000353         4951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_710282, whole genome shotgun sequence.
ACCESSION   DATIAL010000353 DATIAL010000000
VERSION     DATIAL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710282"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VLA60_10925"
     CDS             complement(<1..1838)
                     /locus_tag="VLA60_10925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSF09921.1"
                     /translation="MATFERLHHLLEQSLRDFQAESSDLSDTQEQELTQALHRLRGRL
                     TQILSTNSDPPPLANLLPVHDQRVSLPSPITLPEFSQIHFVTSCDGVILMANEVGSHI
                     LGMDLAEIGQVSIADYVAHEEWTILRSHLCAIDAPPKLLNRVLTILPPGRPSQKIPCV
                     VVPMFDQSQKVVAWHWELTLEPERSSVQPFIQLVQSLESQLFAGQSMDVCLTQICDAL
                     VRVLGFAFVWLTTMRKGCVPQLRAHAMSGDFDWEAQGALWWSQVSQQGELIQACKGSE
                     ISLLSPCCPSTNAMAWFPPGFQFHQAWGVPLAFDEDCFGMLVVCSEGPQPVDPTVLGW
                     LQVLGCEIERLMARGRQLDLWRLQSVAMGSVFDPVCMTDVQGRLEWVNDAYAALHGSS
                     MQSMLGSQLSSFPHEELQDRKSMSDPSAQEFGCLKTEVMQTGSNGQSLVFEQVVTPLL
                     NEQGEAMHFAVILHDVTARKTQELLMKHQAYYDPLTDLPNRIMFNDRLEQALAQARRN
                     GTLLALFFLDLDNFKTINDYHGHQTGDRVLRIVAERLATCVRSTDTVARLCGDEFTVI
                     LQGIEHIQDIRQVALKILECLIPPIRLGKEKIPIQISIGISVYPKDS"
     gene            complement(2053..2778)
                     /locus_tag="VLA60_10930"
     CDS             complement(2053..2778)
                     /locus_tag="VLA60_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HSF09922.1"
                     /translation="MILAAGLGTRLRPLTNTIPKPLLPVAGTPLIIWNLLLLRTSGIR
                     DVIINLHYLGNMIEEAIGDGSRWDMQVKYSHETTLLGTGGGLKAAEWFFEQHPFLVIN
                     GDTLIELDVKALVEFHRTHGGVATLVLRDDPQAAQWGAVDSDAQNRIFRINGRGMSQA
                     ELPNDLVQRMFAGVHILHPSILHNAPADRPFSIIDSYTNELARGSNIFGFLHTAYWSD
                     IGTVARYAQAQADAEAGVISPKT"
     gene            complement(2798..3907)
                     /locus_tag="VLA60_10935"
     CDS             complement(2798..3907)
                     /locus_tag="VLA60_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HSF09923.1"
                     /translation="MTPQTSLSLTGLDLSGVSSTLRKHLPFTAELEQCVPLAGDASNR
                     RYYRLHLSMAPVSSVILMKLADPEGFKASEEAVSGAGGDVTELPFTNILKHLRKNGVQ
                     VPVLYFYDESCGLLYLEDFGDVTLAQAWQESTPAIGENLYCKAIDQLVQLHLQASHPS
                     SDPCVALTRSFDVPLYLWEFDHFLEYGIVVRERPMRVDDQVPVREEFQKMAEWLASQP
                     QVFTHRDYHSRNLMVDGERLGVIDFQDALMGPVTYDLASLLRDSYVALDEGVIDRLIA
                     RYVEGMRQNLPFSQQSAMLFHDPEAFRRLFDFTSIQRNLKAAGRFVYIDRVKGNSSFL
                     ASIPQTLKNVRANLVKYPQLHRLLTHLSPYIPEWR"
     gene            4698..>4951
                     /locus_tag="VLA60_10940"
     CDS             4698..>4951
                     /locus_tag="VLA60_10940"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HSF09924.1"
                     /translation="MSYKNIGDYGIIGDLHTIALVGIDGSIDWCCLPRFDSPSLFGAI
                     LDHKIGGYFKIAPVTNGNTRQMYLPETNILLTRFLQHDGVG"
BASE COUNT         1415 a         1292 c         1102 g         1142 t
ORIGIN      
        1 gaatccttag gatagactga aatcccaata ctaatctgta tagggatttt ttcctttccc
       61 aatcgaatgg gcggaataag acattctaaa attttcaggg ccacctgacg aatatcctga
      121 atatgttcaa tcccttgaag aatcacggta aattcatccc cacacaatcg cgcgacggta
      181 tcggttgatc gcacacaagt tgccaaccgc tccgccacga tacgcaatac gcgatctcca
      241 gtttggtgac catgataatc attgatagtt ttaaaattat cgagatccaa aaagaataat
      301 gccagtaagg tcccattccg cctggcttgc gccaacgcct gttccaggcg gtcgttaaac
      361 attattcgat tcgggagatc ggttaacgga tcgtaatagg cctgatgctt catcagcagt
      421 tcttgtgtct ttcgagcggt cacatcatgc aagatcaccg caaaatgcat tgcttccccc
      481 tgctcattga gcaaaggagt aaccacttgc tcgaaaacca gagattggcc attgcttccc
      541 gtttgcatca cctcggtctt caagcaaccg aactcctggg cagatggatc tgacatagac
      601 ttcctgtctt gcagttcctc atgagggaac gaactcaact gagaacccaa catcgattgc
      661 atcgaggagc catgcaaggc ggcataggca tcatttaccc attccaaacg accctgcacg
      721 tctgtcatgc agactgggtc aaatacggag cccatagcca cactttgtaa tcgccataga
      781 tccagctgtc ttccacgtgc catcaaccgt tcgatctcac aacctaatac ttgcaaccac
      841 ccaagcaccg tcggatcaac cggctgtgga ccttcagaac acaccaccag cataccgaaa
      901 cagtcctcat caaaagccag tggaacaccc caggcttgat gaaactggaa tccaggcgga
      961 aaccaggcca tggcatttgt ggaaggacaa catggagaaa gcaatgatat ttcagaaccc
     1021 ttgcaggcct gaattaactc accttgctgc gaaacctggc tccaccacag agccccttgc
     1081 gcctcccaat caaaatcgcc agacatcgca tgcgcccgta actggggcac acatcccttt
     1141 cgcatggtgg tcagccatac aaaggcaaaa cccaaaaccc gcacaagggc atcacaaatc
     1201 tgcgtcagac acacatccat actctgaccg gcaaatagtt gcgattcgag actctgcacc
     1261 aattggataa aaggctgaac ggatgagcgt tctggctcca gagtcaattc ccaatgccac
     1321 gcaaccacct tctgggactg atcaaacatg ggcactacca cacatgggat cttttgcgat
     1381 ggtctccctg gcgggagaat tgtcagcact ctattcaaga gcttcggagg agcatcaatc
     1441 gcacaaagat ggcttcgaag aatcgtccat tcctcatgag caacataatc ggcaatggac
     1501 acctgcccta tttcagccag gtccatcccc aggatatgac tacccacctc attggccatg
     1561 aggatcacac catcacacga ggtcacgaaa tggatctgag agaattctgg gagagtaatc
     1621 ggagacggga gcgacaccct ctgatcgtga accggaagta aattggcaag aggaggtggg
     1681 tcggaattgg tcgaaagaat ttgggtgagt cgacccctca atcggtgaag tgcctgagtg
     1741 agttcctgct cttgcgtatc ggaaaggtca ctcgattcag cttgaaaatc tctcaaggac
     1801 tgttcgagta agtgatggag tcgttcaaaa gtggccacaa ttaccttcac cctggcacaa
     1861 ggaatgggag aaccatgggg agtatttaga taataaaccc gatatcgata tcttttggca
     1921 aggtttcctg gatgtcaacc cgtcaaaaga ggtgcgatta ttggcgccaa ttcctcacca
     1981 acgtactcag agcatcagcc gacaactcaa tccatagagg gaaatagtgt acagcctaac
     2041 aggcgaccat tcttaggtct taggggaaat cactccagcc tccgcatcgg cttgcgcctg
     2101 agcataacgt gccaccgttc caatatcgga ccaataggca gtatgaagaa atccaaatat
     2161 atttgaaccc cgcgcaagct cattcgtata ggagtcgatg atggaaaacg gcctatccgc
     2221 gggggcattg tggaggatag aaggatggag aatatgaacg cctgcgaaca tccgttgaac
     2281 caggtcgtta ggcaattcag cttggctcat tccccgacca ttaattctga aaatacgatt
     2341 ttgtgcgtcc gaatctacgg ctccccattg ggctgcttgc ggatcgtcgc gaagcactaa
     2401 cgtggccaca ccaccatgag tccgatgaaa ctctaccaat gccttcacat ccaactcgat
     2461 caacgtatcc ccattaataa ccaaaaacgg gtgttgctca aaaaaccact cagccgcttt
     2521 taatcctcca cccgttccta acaaggttgt ttcatgggaa tacttcacct gcatatccca
     2581 acgggatccg tcaccgatcg cttcttcgat catatttccc aaataatgca gattgatgat
     2641 gacgtcacgt attccagatg ttcgcaataa caacaaattc caaataatga gcggcgttcc
     2701 cgcaaccggc aataacggtt tgggaatcgt gttggtcaag ggacgaaggc gagtgccaag
     2761 acccgcggcc aaaatcatcg ccttcatgtg caagaagcta acgccattca gggatatacg
     2821 gagacagatg ggtgagcagt cgatggagtt gaggatattt tactaaatta gcacgaacat
     2881 tctttaatgt ctggggaatt gaggccaaaa aacttgaatt accttttact cgatcaatat
     2941 acacaaaccg accggcagcc ttcagattgc gctgaatact cgtgaagtca aatagccgac
     3001 gaaacgcttc aggatcatgg aataacatag ccgattgttg cgaaaatggg agattctgac
     3061 gcatcccttc tacgtaacga gcaataagac gatcgattac cccttcatcg agagcgacgt
     3121 aggaatccct caaaagggag gccaaatcat aggtcacagg gcccatcaag gcatcttgaa
     3181 aatcaatgac gcccagccgt tccccatcca ccatcaagtt acgggaatga taatcacgat
     3241 gggtaaaaac ctgcggttgc gaggctaacc attcggccat cttctgaaat tcctctcgaa
     3301 ccggcacttg gtcgtcgaca cgcatgggcc gctctctcac cacaatgcca tattccaaaa
     3361 aatggtcaaa ctcccagaga tacaggggaa catcaaaaga ccgtgtcaac gccacacagg
     3421 ggtcactgga aggatgtgag gcctggagat gaagctgaac gagttgatca atagccttac
     3481 agtatagatt ctccccgata gccggggtag attcctgcca tgcttgggcc agcgtcacgt
     3541 ctccaaaatc ttcgaggtag agcaagccgc aggattcatc ataaaaatat aacacgggaa
     3601 cctgaacgcc attcttccgc agatgtttca gaatattcgt gaacggcaat tcagtgacgt
     3661 ctcccccggc tccacttacg gcctcctcag aagctttaaa cccttccgga tcggctaact
     3721 tcatcagaat cacagaggaa acgggagcca tggaaaggtg caaccggtaa taacgacgat
     3781 tcgaagcatc ccccgccaac ggcacgcatt gctctagttc ggcagtgaac ggaagatgtt
     3841 tacggagagt agaggacaca cccgagagat cgaggcccgt caaagacagg gaagtttgag
     3901 gcgtcattac tttcgattat acggacatac caacttcttg tcatcaagag gaattaggat
     3961 acattcattt taacaactga attcccctgc ctaggttaca atggcttagc ttcgcggaac
     4021 ggaaaaaaag cccgcgcgtc accaaatgac gacctgcctg cttgcttgcg agtcgaagcc
     4081 gattcaccgc aaaaaggcga agtctctccg agaaggatgg gaacaatgct ggcgatatct
     4141 tcccatctta tacctccatt taagattgag aacattttga cgataagtag tatgaagaga
     4201 gggaaaattc tcacaagtca ccataatcac ccacaaatgt aaaggtgctc tatggaaatc
     4261 accaatcccc ccctgccaac tctcccctcc actcaatcta cctcctcggt ccaggatgga
     4321 gatgtgggcc agaagggtag cttttacagt cgcaacagaa aaaaacaaca caaggataat
     4381 gagcaacact cggaagaacc accaccccgc aaagatcgct caacacgact cattgatatc
     4441 cgcgtgtagc actggttaat tcttgaatgt tcatgtttcc tacgcgccga aacaatgtcg
     4501 tcccttcgga ttttgatcac aggattccat actctgcaga tgatcatatt ggggaccccc
     4561 tcctatctaa atcaaaatct aaatattgag ccttatcgat aaatttatcg ctatatctag
     4621 tgtttactgt tcactttttg ggtatattgg cttttggatt tcttgattta gccattttca
     4681 cctaatgaga accggacatg agctataaaa acatcggtga ttacggcatt atcggagatc
     4741 tccacaccat tgcccttgtt gggatcgatg gatcgattga ttggtgttgc ctgccacgat
     4801 ttgactcccc tagcctgttt ggagccattc ttgatcataa aattggaggg tatttcaaga
     4861 ttgcccccgt cacaaatggc aatacccggc aaatgtacct gccggaaacc aatattctgc
     4921 tgacccggtt tctgcaacat gatggtgttg g
//
LOCUS       DATIAL010000354         4640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_281911, whole genome shotgun sequence.
ACCESSION   DATIAL010000354 DATIAL010000000
VERSION     DATIAL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281911"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..490)
                     /gene="rplL"
                     /locus_tag="VLA60_10945"
     CDS             complement(116..490)
                     /gene="rplL"
                     /locus_tag="VLA60_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HSF09925.1"
                     /translation="MSKEDFIKVIEGMSVLELSDLVKGLEERFGVTAAAPVGVMAAAV
                     GAAAPAAEEQTEFTVVLGSAPADKKIQVIKVVREITGLGLKEAKDLVEAAPKAVKEGV
                     SKEESETIKKKLQEVGATVEVK"
     gene            complement(545..1051)
                     /gene="rplJ"
                     /locus_tag="VLA60_10950"
                     /pseudo
     CDS             complement(545..1051)
                     /gene="rplJ"
                     /locus_tag="VLA60_10950"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(1082..1774)
                     /gene="rplA"
                     /locus_tag="VLA60_10955"
     CDS             complement(1082..1774)
                     /gene="rplA"
                     /locus_tag="VLA60_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HSF09926.1"
                     /translation="MGKKFESALAKVTKALYSLEEASDLVKQAAFAKFDETVELSLCL
                     GVDPKRADQMVRGTTVLPHGTGKKVRILVIAKGEKEQEARDAGADFVGSDDLLQKIQD
                     GWLDFDSMIATPDMMGAVGRMGKVLGPRGLMPNPKTGTVTFEVGKAIQEIRRGRVEYK
                     VDKAGNIHVPVGKVSFQGQQIRENAQSVFDSIMKAKPSSSKGKYVKSATLSSTMGPGI
                     HLDGAAIAKQVS"
     gene            complement(1811..2236)
                     /gene="rplK"
                     /locus_tag="VLA60_10960"
     CDS             complement(1811..2236)
                     /gene="rplK"
                     /locus_tag="VLA60_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HSF09927.1"
                     /translation="MAKEVTSQIKLQIPAGKANPAPPVGPSLGQHGVNIMEFCKQFNA
                     RTQKQEGSIIPVVISVYKDRSFSFVTKTPPASDLLKKAAGIIKGSGIPHKNKVGTITM
                     AQLEDIAKLKVEDLNASDIPAAMNVIAGTARSMGITVEG"
     gene            complement(2302..2838)
                     /gene="nusG"
                     /locus_tag="VLA60_10965"
     CDS             complement(2302..2838)
                     /gene="nusG"
                     /locus_tag="VLA60_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HSF09928.1"
                     /translation="MSSHWYVIHTYAGYEGRVRAGIVERANQLGMADSVAQVLVPTED
                     VVEFKEGKRRASKRKFFPGYVLLEANGPLGDEVVTMIKETPKVTGFIGGGTRPIPLDP
                     EEVATLLKQLESGVTAPRELANFSKGDNVRIVDGPFLGFNGLVDEVDQDHGRVKALVS
                     IFGRSTPVELAFSQVERV"
     gene            complement(2859..3053)
                     /gene="secE"
                     /locus_tag="VLA60_10970"
     CDS             complement(2859..3053)
                     /gene="secE"
                     /locus_tag="VLA60_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HSF09929.1"
                     /translation="MFKKIWFSITEFLSDVRGELKKISYPTKSETIGSTTVVLLFCVI
                     MSLYLSVVDSFLVWLISRII"
     gene            complement(3144..3219)
                     /locus_tag="VLA60_10975"
     tRNA            complement(3144..3219)
                     /locus_tag="VLA60_10975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3183..3185),aa:Trp,seq:cca)
     gene            complement(3257..3406)
                     /gene="rpmG"
                     /locus_tag="VLA60_10980"
     CDS             complement(3257..3406)
                     /gene="rpmG"
                     /locus_tag="VLA60_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HSF09930.1"
                     /translation="MRVIISLACGDCKRRNYSTMKNKKNDPDRLEVKKYCRFCRKHSP
                     HKEVK"
     gene            complement(3425..4630)
                     /gene="tuf"
                     /locus_tag="VLA60_10985"
     CDS             complement(3425..4630)
                     /gene="tuf"
                     /locus_tag="VLA60_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HSF09931.1"
                     /translation="MAKAKFERKKPHVNVGTIGHVDHGKTTLTSALTKVMGDTGKAKF
                     VPYDEVAKASESQGRRDPTKILTIAISHVEYETDNRHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAADGPMPQTREHILLARQVGVPFIVVFLNKADKVEDKELLELVELEVR
                     ELLTKYGFPGDDVPVVIGSAIKAIEGDQSEVGVPSIMRLLEAVDSYIPTPQRAIDKPF
                     LMPIEDVFTISGRGTVVTGRVERGVVKVGDEIEIVGLKPTQTTIVTGVEMFRKVLDEG
                     QAGDNIGALLRGTKKEDVERGMVLAKPKSITPHTKFKAEVYILTKEEGGRHTPFFNGY
                     RPQFYFRTTDVTGVVQLNPGVEMVMPGDNVSFEGELISPIAMEQGVRFAVREGGRTVG
                     AGVVTEIVA"
BASE COUNT         1113 a         1483 c          844 g         1200 t
ORIGIN      
        1 tcaaaggctg gttgtcccat tcctgtctcc ttaaggatta acggccacct cagcttcctg
       61 ctggccctcg aaccttccta agtctctatt ccgaatcctc aaaatgaggg aaacactact
      121 tcacctctac ggtcgcacca acctcttgaa gtttcttctt aatagtttct gattcttctt
      181 tggacacgcc ctcctttacc gccttcgggg ccgcctcaac gagatcctta gcttccttca
      241 aacccaaccc agtaatctct cgcaccacct tgatgacttg aatcttttta tcagccgggg
      301 cacttcccaa tactaccgta aactcggtct gttcttcagc ggcaggagcc gcagcgccca
      361 ctgcagcagc catgacccca accggcgccg ctgccgtcac accaaatcgc tcctccagac
      421 ccttcacaag atccgacaat tccaggacac tcatgccctc tataacttta atgaaatctt
      481 cctttgacat ttttgcctct gttgcgctca tctccccttc tcctttctgc gttttgtttt
      541 cttgtattgc ggctaacacc gcaacaatac ttcttacaat ttgacctaaa accccgacaa
      601 accctcgaat tggcccttgg aaggccgaca acaacatcga caacaattct gcttttgacg
      661 gcaaatccgc cacagaagag agacgagctg gatttaactc gaccccgtcc agaattgccc
      721 ctttgagctg caatttctct tcacattttt cacttttaat aaaatcccga attactttcg
      781 caggcaatac cggatcatcg tatccaataa ccaatgccga ttgaccctta aaaaatggtt
      841 gcaaaggaac aagggccgtt cccccaatag ctctcacggc aagggtgttc ttgaccacat
      901 gaaagtttga cttcgcctga cgaagttttt ttcgcaaatc attgatttga ttcacgggca
      961 tacccgcgca ttccgttaca atggctatcc gcgctcgagc gaattgctca tgcatgactg
     1021 ccacagcttc atttttatca gtttttttca tacctcgaac tcacctgctg agattaaagt
     1081 ttcaactgac ctgctttgca atcgcggctc cgtccagatg gatacctggt cccatcgtgc
     1141 tggacaatgt cgcacttttc acatacttcc ctttactgga ggagggctta gccttcatga
     1201 tggaatcaaa aaccgactga gcattttctc taatttgttg cccctgaaaa gacacctttc
     1261 caacaggaac atgaatgttg cctgccttat caaccttata ctcaactctc cccctcctga
     1321 tctcctgaat ggctttcccg acctcaaagg tcaccgttcc agtctttggg tttggcatta
     1381 atcctcgtgg ccccaacacc ttccccattc ttccgacagc acccatcatg tccggggtcg
     1441 caatcatgga gtcaaaatcc agccatccat cttggatttt ctgcaaaagg tcatcgctcc
     1501 ccacaaaatc ggcgccagcg tccctggcct cctgctcctt ctcccctttt gcaatcacta
     1561 aaatcctgac ctttttcccc gttccgtgag gaaggaccgt agtcccgcga accatctgat
     1621 ccgcacgctt gggatctacc ccaaggcaaa gtgacagctc taccgtctca tcaaacttgg
     1681 caaaagcagc ctgttttact aggtcactgg cctcctctaa tgaatatagg gcctttgtta
     1741 ccttcgctaa agccgattca aatttctttc ccacggcgca taccctctgt taaaagcctc
     1801 acaatattat ttacccttca actgtaatgc ccatacttct cgcggttcct gcaatcacat
     1861 tcatcgcggc tggtatatca ctggcattca gatcttctac cttcaactta gcaatgtcct
     1921 ccagctgtgc catggtgatt gtcccaactt tatttttatg gggaatccct gaacccttta
     1981 tgatacctgc agccttcttc agtaaatctg atgccggagg agtcttggtc acgaagctga
     2041 agcttctgtc cttataaaca gaaatgacca ccgggataat gctaccctct tgcttctgag
     2101 ttctagcatt aaactgttta caaaactcca taatgttcac tccatgttgt cccaaggaag
     2161 gcccaacagg aggagcagga ttggcttttc cagccggaat ctgcaattta atttgactag
     2221 ttacttcttt ggccatgcct tcacctacct tacatcgact tgcgtattgc ttaaattaaa
     2281 aaaggaatac tccacttgtc ttcaaacacg ctcaacctga gaaaaagcca attccacagg
     2341 ggttgaacga ccaaaaatac tgaccagcgc cttgacccgg ccatgatcct gatccacctc
     2401 atccactaat ccattgaaac ccaaaaacgg gccatccaca attcggacat tgtccccttt
     2461 tgaaaaattg gccaactccc ttggagccgt tacgccggat tccaactgct tcaagagtgt
     2521 ggccacctcc tcgggatcaa gcgggattgg cctggttccg cctccaataa aaccggtcac
     2581 cttgggggtc tccttgatca tagtaacgac ctcatcccca agcgggccgt tcgcttcaag
     2641 cagaacatac ccgggaaaaa attttcgttt agaggcccgg cgttttcctt ctttaaattc
     2701 cactacatct tcagtaggga caaggacttg cgccacagaa tctgccatac ccaattgatt
     2761 tgcccgctcg acaattccgg cccgaacccg cccctcatat cccgcatagg tgtgaatgac
     2821 ataccaatga ctcgacatga attcccttct aatagatact aaataatcct actaattaac
     2881 caaacaagaa atgaatccac cacagagagg tacaaggaca taatcacgca aaacaacaaa
     2941 accaccgtgg tcgacccgat cgtctcagac ttagtaggat aagaaatttt tttaagttcc
     3001 ccccgcacgt cagacaaaaa ctcggtaatg gaaaaccata tcttcttaaa cacgacttac
     3061 cccctcaccc tttttcctaa cccctccact acgccaacaa acgactcttg ctcttttttg
     3121 cacatgacat tcacagccaa gtatggcagg ggcacaggga ttcgaacccg gactttcggt
     3181 tttggagacc gacgtgctag ccgttgacac catgcccctc caacctgcac gcaacctaaa
     3241 tgacccatac caccaattac ttgacctctt tgtgagggct atgctttcga caaaatcggc
     3301 agtatttttt tacttccaac cgatctggat cgtttttctt gtttttcatt gtcgaataat
     3361 ttcggcgctt gcaatctccg cacgctagag aaataatgac tctcatccat acacccctat
     3421 gttcttacgc gacgatttcc gtgaccaccc cggctccgac cgttcggccc ccttctcgca
     3481 ctgcaaatcg caccccttgc tccatcgcaa tcggggagat cagctcaccc tcaaacgaca
     3541 cattatcccc cggcatcacc atctcgaccc ccgggtttaa ttgcaccact cccgtgacat
     3601 ccgtcgtccg gaagtaaaac tgcggacgat agccattaaa aaacggcgta tgccgcccgc
     3661 cctcttcctt cgtcaaaata tacacttccg ccttgaattt cgtatgcggc gtaatgctct
     3721 tcggcttggc caacaccatg ccgcgctcca catcctcttt cttcgtgccc cgcaacagcg
     3781 ccccaatatt gtcacccgcc tgcccctcat ccaatacctt ccggaacatt tccacgcccg
     3841 tgacaatcgt cgtctgcgtc ggtttcagcc ccacaatctc gatttcatcc cccaccttga
     3901 ccacgccgcg ctccacccgg cccgttacca ccgtcccccg cccgctgatc gtaaaaacat
     3961 cttcaatcgg catcagaaac ggcttatcaa tggctcgctg cggagtcggg atgtagctat
     4021 cgaccgcctc caataagcgc ataatcgacg gcacgcccac ctcactctga tccccttcaa
     4081 tggccttgat cgctgacccg atgaccaccg gcacatcatc gcccggaaag ccatacttcg
     4141 tgagcaactc ccgcacttcc aactccacca actccagcaa ttccttgtct tccaccttat
     4201 ccgctttatt caaaaagacc acgataaacg ggacccccac ctgccgcgcc aacaaaatat
     4261 gctcccgcgt ttgcggcatc ggcccatcgg cggcactcac caccaaaatc gcgccgtcca
     4321 tttgcgcggc cccggtaatc atgttcttca cataatccgc atgccccggg cagtcgacgt
     4381 gcgcataatg tcgattgtct gtttcatact ccacatggga gatcgcaatg gtgagaattt
     4441 tggtcggatc ccgtcgcccc tgactctcac tcgccttggc gacttcatca taggggacaa
     4501 acttagcctt ccccgtatcg cccatcactt tcgtcaaggc cgacgtcagg gtcgtcttcc
     4561 catggtcaac gtgcccgatc gtccccacat tgacatgcgg cttcttccgc tcaaatttcg
     4621 ccttcgccat gccctacctc
//
LOCUS       DATIAL010000355         3576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1248030, whole genome shotgun sequence.
ACCESSION   DATIAL010000355 DATIAL010000000
VERSION     DATIAL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248030"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1642)
                     /locus_tag="VLA60_10990"
     CDS             complement(65..1642)
                     /locus_tag="VLA60_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF09932.1"
                     /translation="MELTTQQNLVHQTRDARRSKSFPKLSPSERDSLIKKYHPDHRAH
                     AYRPIKFGPNAGQLTVTEVATLLEGESPVPADLDLSPEYSVDVLVIGGGGAGCASALH
                     AHAQGADVLLATKLRLGDSNSVMAQGGMQIAVAPDDSPVQHFLDTLKGGHMKNDHQLL
                     KVMVEEGPAIAKWLLELGVLFDRDADGNLHVKKGGGSSKARLLTCSDYTGLEIMRVLK
                     DEVLNKKIPLLEFVAAVELLSDTKGACTGAVLKDLDNNRFVVVAAKTVILATGGIGRL
                     HIQGFPTSNHFGATGDALVLAYRLGVPLVQIDTFQYHPSGGVYPEQLVGALVTEGIRS
                     EGGHLVNAKGERFVNELDTRDVVSSSIIRECEEGRGVRTPSGRVGVWLDTPLLDVEHG
                     PGTLDKHFPAMVRQYERYQVDIRKDPVLIYPTLHYQNGGVKIDVNGETPVANLFVAGE
                     ASGGLHGRNRLMGNSLLDLMVFGKRTGTLAAERAKSLPKASLTLEHLRRFRAEAKRHH
                     VSAGVISPMVLPAYTRK"
     gene            complement(1663..2379)
                     /locus_tag="VLA60_10995"
     CDS             complement(1663..2379)
                     /locus_tag="VLA60_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSF09933.1"
                     /translation="MSDALDKEQLLDQEETLQPKMVTVEIEGKRFQVPAGITLIKALW
                     YTGQDAIRGVGCLGGFCGACATYYRTKDDPKVKTCLACSMAVEDGMSFSFMPAFPARK
                     ATYNLLELKDPKQDLFELYPEAPLCRNCNACTEACPQHIDVREGVWKAVFGDFKAVSE
                     MFMDCVMCGMCAPVCIADIAPNLVALYASRAQGVHFTEKPEGLAKRIQEISDGRFQKE
                     WDHILKLSDEELQNTTASTT"
     gene            complement(2459..>3576)
                     /locus_tag="VLA60_11000"
     CDS             complement(2459..>3576)
                     /locus_tag="VLA60_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HSF09934.1"
                     /translation="GLKAKTAFLVTPGSEQIYHTMKRDGFLATFESMGATVLANACGP
                     CIGQWKRADAVKGKADSIVSTFNRNFPGRNDGVSDTLSFLASPEVVTAYAFSGDLAFD
                     PVKGTLKTADGKDFKFEAPQGDELPAKGFAKGEEGFVAPAEDGSALKVDIPPTSERLQ
                     LLQPFPRWDGKDFDKLPLLIKTKGKTTTDHISPAGPWLKFRGHLDKISDNMFLGANNA
                     FSDEAGKGNDVLTGESGLTLAQIARRYKEKGIGSFVVGDENYGEGSSREHAAMSPRFL
                     GVKAVLTKSFARIHETNLKKQGILPLTFADAKDYDQIEQEDRISVKGLNTLAPGKPVQ
                     VILHKKNGQEVEIQANHSMTEQQITWFKAGSALNALN"
BASE COUNT          850 a          987 c          821 g          918 t
ORIGIN      
        1 atggcggaga tcagaacatt ggccgatgac gatcatccgg gtggttatct atacgattcg
       61 cttcttattt tctggtatag gctggcaaga ccattgggga gatgacacct gccgacacat
      121 gatgacgttt ggcttcggct cgaaacctcc tcaggtgctc taatgttaag gaggctttcg
      181 gcagggactt agctcgctcg gcagcaagag taccggttcg cttgccaaag accattaggt
      241 ccaataagga attccccatc aagcgatttc gcccatgcag gccaccggag gcttctcccg
      301 cgacaaataa attggcaaca ggcgtctctc cattcacatc aatcttgacg cccccgttct
      361 gatagtgcaa cgtaggataa atcaacaccg gatctttccg aatatctacc tggtagcgct
      421 catactgccg gaccatagcg ggaaaatgtt tatccagcgt gcccggtcca tgttcaacat
      481 ccaacaatgg cgtatcgagc cacactccca ctcttcctga tggtgttcgg acgcctcgcc
      541 cctcttcaca ttcccgaatg atcgacgagg acacgacatc ccgagtatcc aattcgttca
      601 caaagcgttc acccttcgcg ttgactaaat gcccgccttc agatcggatc ccttcggtga
      661 ccaatgcgcc gactaattgc tcaggataca ccccgccgga agggtgatat tgaaacgtat
      721 cgatttgaac gagaggaact ccgagacgat aagcaaggac aagggcatcc cctgtcgccc
      781 cgaaatgatt gctggttgga aatccctgta tgtgcaaccg gccaatcccg cctgtcgcca
      841 gaatgactgt tttagctgcc acgacaacga agcggttgtt gtccaaatcc ttaagaaccg
      901 cgcctgtgca ggctcctttg gtatcgctta acaattccac agcggcaacg aattccaata
      961 agggaatttt tttgttcagg acctcgtcct tcaacacccg catgatttcc aagccggtat
     1021 aatccgaaca ggtcaggagt ctggcctttg aactacctcc cccttttttg acatgcagat
     1081 ttccatccgc atcccgatcg aataacacgc ccaattcgag cagccatttc gcaatagccg
     1141 gcccctcttc caccatgacc tttaatagct ggtggtcgtt tttcatgtgt ccgcccttta
     1201 acgtatccag aaaatgctga accggcgaat catcgggggc cacggcaatc tgcattcccc
     1261 cctgggccat cactgaattg gaatcgccca gtcgtagctt ggtggccagc aatacatcgg
     1321 ccccctgggc atgggcatgg agtgccgacg cgcacccggc tcctcctcca ccgatgacta
     1381 agacatccac ggaatattcc gggctgagat cgaggtcagc cgggaccggg ctttcccctt
     1441 ctaacaatgt cgcaacttcg gtgaccgtta attgaccggc attggggccg aatttgattg
     1501 gtcgatacgc gtgagcgcgg tggtcaggat gatatttttt aatgaggcta tcgcgttcgg
     1561 acggagaaag ctttggaaag cttttggatc gacgggcgtc tctggtttgg tggaccagat
     1621 tttgttgggt ggtgagttcc atagggaagg agattgcgtc ggttaggttg tcgaggcggt
     1681 agtgttttgc aattcctcat cagacagctt gaggatatga tcccattcct tttggaacct
     1741 cccatcggaa atctcttgaa tgcgtttggc aagaccttcc ggtttttcag tgaaatgcac
     1801 gccttgagcc cggctggcgt aaagcgccac cagattggga gcgatatcag cgatacaaac
     1861 aggcgcacac atgccacaca tgacacagtc cataaacatt tcagaaaccg ccttgaaatc
     1921 gccaaacact gctttccaca ccccttctcg aacatcgata tgttgaggac aagcttcggt
     1981 acaggcatta cagttccggc aaagcggggc ttccggatac agttcaaaca aatcctgttt
     2041 aggatcttta agctcaagca ggttatacgt cgctttcctg gccggaaaag caggcatgaa
     2101 agaaaaggac atcccatcct caaccgccat cgaacaggct aaacaggttt tgaccttggg
     2161 atcgtccttc gtgcggtaat aggttgcaca ggctccacaa aatccgccta agcagccgac
     2221 gccacgaatg gcatcctgtc ctgtatacca caaggctttg atcagggtta tgccggctgg
     2281 aacttggaaa cgtttccctt caatctccac cgtcaccatc ttgggttgaa gggtttcttc
     2341 ttgatccagt aactgttctt tatctaatgc gtcagacata attacgaatc atctttgatt
     2401 cttaacaatt tttgcggggt attttaacaa tctcactcac ccacgccgtt tatggaactt
     2461 aattcaatgc attcaacgcc gagccggcct taaaccaggt gatttgttgt tcagtcatac
     2521 tatgattggc ttgaatctct acctcctgcc cattcttttt atgaagtatc acctggaccg
     2581 gttttcccgg agcaagggtg tttaaccctt ttacactgat gcgatcctct tgttcgattt
     2641 ggtcataatc cttggcatcc gcaaacgtca gtggaaggat gccctgtttc ttaaggtttg
     2701 tttcgtgaat gcgggcaaaa ctttttgtca agaccgcttt gactcccagg aaccggggag
     2761 acatcgccgc atgttcccga ctgctccctt caccatagtt ttcatctcca accacaaacg
     2821 acccaattcc tttctcttta taccttctgg caatttgtgc gagggtcaat cctgactcac
     2881 ccgtcaaaac atcattaccc tttcccgcct cgtctgaaaa tgcattattg gcccccagaa
     2941 acatgttatc gctgatttta tccaaatgtc cgcgaaattt gagccacgga ccggcgggag
     3001 agatatgatc ggttgtggtc ttccctttgg ttttaatcaa taacggcagc ttatcgaaat
     3061 cttttccatc ccaacgaggg aagggttgca ataattgcag ccgctcactt gtgggaggaa
     3121 tgtccacttt aagcgccgag ccatcctcag caggagcaac aaatccttct tctccttttg
     3181 caaaaccttt tgccggaagc tcatcaccct gaggcgcctc aaatttaaag tcttttccgt
     3241 ctgcggtctt cagcgttcct ttgacaggat caaaggccaa atccccggaa aatgcatagg
     3301 cggttaccac ctccgggcta gccaggaagg acaacgtgtc actcaccccg tcatttcgac
     3361 cgggaaaatt ccgattaaac gtactcacaa tcgagtccgc tttgcctttc accgcatctg
     3421 cccgcttcca ttgcccaata caggggccac aggcattggc caagacagtg gcccccatgc
     3481 tctcaaatgt cgccaaaaat ccgtcacgct tcatggtgtg ataaatttgc tcagatcccg
     3541 gagttaccaa gaaagctgtt ttggctttga gcccgg
//
LOCUS       DATIAL010000356         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_173312, whole genome shotgun sequence.
ACCESSION   DATIAL010000356 DATIAL010000000
VERSION     DATIAL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173312"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..729)
                     /locus_tag="VLA60_11005"
     CDS             complement(385..729)
                     /locus_tag="VLA60_11005"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF09935.1"
                     /translation="MLQVCEKLNHQSTVIIFTGRFDRQSTIGIGDLILGTKGMRCQHI
                     ILDFRGITGIDSVGLGLLYLWYHKLQPQHVGLSIVGPSPNVRAALEGCHFPDRVPFYA
                     SEHEAVRQEIYG"
     gene            complement(1386..1474)
                     /locus_tag="VLA60_11010"
     tRNA            complement(1386..1474)
                     /locus_tag="VLA60_11010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1438..1440),aa:Leu,seq:taa)
     gene            complement(1687..2763)
                     /locus_tag="VLA60_11015"
     CDS             complement(1687..2763)
                     /locus_tag="VLA60_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF09936.1"
                     /translation="MWVCPNRSNLIVVVMLGVLMASGCSSHSVRTGANSEGDGMASSE
                     DGNAVDPNRIMSQANGSVEGSGRNGGLVNGDKASQYANGSHSQDRVNGSNPNSNPETI
                     GGEERRLGQSGSNYGHQYPGVDGSGPNHGPVSGFENGMASSQNPDPEAWANAYLGNGK
                     NSRDFYGDPSKSAALNDGSSQGGVNPATWAETYKKEHGGPSPEYVDPDSSIANGQGQR
                     GRASVGIGRTDTNGSIFESNFSGSMGIQHGQVQDIYFAFDSWNISSSAAKYLEEGAQW
                     LQANPGKALTIEGHCDQRGTQDYNLVLGQKRADAAREYLVNLGVQPDRIKIVSYGKER
                     PFCQNDSEDCFQENRRNHMVVRVN"
BASE COUNT          874 a          821 c          716 g          803 t
ORIGIN      
        1 aaggtgaatg gttgaaaaaa tgaaaaaata cataaccaat atacaccgga aaagaaacat
       61 gcaaatggta caatctgata atttacgacc caaggtgaag cgtcactctg tcatgctcaa
      121 aacctcccac taaacttcat cctccctgca gatattcatc ctcggtgact atcacatctt
      181 gcgtctgttg tgaaggtttc catgcggatt cgtgacagac taaaccctgg gcatacgata
      241 catacccaca cctgagatgg ccttgaggcg acatatttcc tcaaacaaat tcagagctga
      301 cttatcgtaa attctcaggc cgcggattag aggatcaaag agcacaggat caggccgatg
      361 cctgaaggaa gcagcaaaga cattctaacc gtagatttcc tgtcggaccg cctcgtgctc
      421 agaggcatag aacggaacgc ggtcaggaaa gtgacagcct tccagtgcag ctcggacgtt
      481 aggagaaggc cccacaatac ttagcccaac gtgttggggt tgtagtttat ggtaccagag
      541 gtacaagagc ccgagaccca ccgagtcaat cccggtaatg cccctgaaat ccaggatgat
      601 gtgttgacat ctcatgcctt tggtgccgag gatcaggtcc ccgataccaa tggttgattg
      661 tcgatcaaac cgtcccgtga aaatgatgac tgtggattgg tgattcagtt tttcgcagac
      721 ttggagcata taatctactt gttaatttgt aaagcaccag gatggaaagt tttaatgcgt
      781 aggactatat tcaatccttt ccggatgggc ctaaaaatta ctcgttaaga ctagaggtag
      841 gtatcagtaa ccttttggaa caacctacac acaaaaaagg aacgactcat gaaattgcaa
      901 aaaatgaccg gggatagggg ctatttcctg tcgtgggtaa atgggagtgc ctaggaatta
      961 gtggggggag ggtgaaaacc ggagcaaagc tcaagcgctc gaaaactctc accacgcaca
     1021 cgaccggagg taagcaacaa tagctcaaga cggttttatg atggagaaaa ttggccgcgg
     1081 gcggattaag ggttcaaaga cttttcaagc ctaaaccctc tgatttttca aaccacaaat
     1141 tttaatgctg gtatccttac ggagggatat tcaattcgat gcagcggaaa ggcattcaag
     1201 actgcttctc agaatgttgg cattccagga ctccggtttc atgatttccg gtaaacggcc
     1261 atcaccaata ggcgacgggc ggggatagat catttgactt tcttgcgcac ttccggacac
     1321 gagaccatgg ccaaccttac ccggttcaac agctaccgcg ctgaccttca atctgttgat
     1381 tcaattggtg ccggagaccg gggtcgaacc ggtacgagct tgtaagcccg agggatttta
     1441 agtcccttgc gtctgcctat tccgccactc cggcatctca aagattggcg acgagactgt
     1501 accactcctt aaaaaccggt gcaagatttt caggcagaaa catcggtaga ggataacctc
     1561 atgcatcccc tagtcatttc acttttttgg gtttgatgcc cgacgtacag ctcaaaaaac
     1621 aaaaggatgc gaaggtttcg catcctcttg ttcggggcca ctgagggtcc tatgaaagcc
     1681 gaatccttaa ttaactcgaa caaccatatg attccgccga ttctcctgga aacaatcttc
     1741 actgtcgttt tgacaaaatg gtcgctcttt gccgtaggaa acaatcttaa tccggtccgg
     1801 ttgcacaccc agattcacta aatactccct tgccgcatcg gcccgctttt gaccgagcac
     1861 cagattataa tcctgtgtcc ccctttggtc gcagtgaccc tcaatcgtca aggcctttcc
     1921 aggattcgct tgtaaccatt gcgccccttc ttcaagatac ttggcggcac tgctggaaat
     1981 attccaactg tcaaaggcaa agtaaatatc ctgcacctga ccgtgttgga tccccatgga
     2041 accactaaaa ttcgactcaa atatcgagcc attcgtatcg gttctcccaa ttcccactga
     2101 agcccgccct cgctggccct ggccattggc aatactcgaa tcaggatcga catactccgg
     2161 agaaggaccg ccatgttcct tcttataggt ttcagcccag gtcgcaggat tcactcctcc
     2221 ttgagaagag ccatcattaa gagccgcgga ctttgacgga tcaccataaa aatcccggct
     2281 attcttgccg tttcccaaat aggcatttgc ccaagcttct ggatcaggat tttgactgga
     2341 cgccattcca ttttcaaatc cagacacagg tccatgatta gggccagatc catccacacc
     2401 ggggtattgg tgcccatagt ttgaaccact ttggcccaac cttcgttcct cgcccccaat
     2461 tgtttctgga ttcgaattgg gatttgaccc gttgactctg tcttgactat gggaaccatt
     2521 tgcatactga gaagctttgt ctccattgac caatccacca tttcttccac ttccctcaac
     2581 tgatccgttg gcctgactca taattctatt gggatcgact gcatttccat cttcacttga
     2641 agccatccca tccccttccg agttggcccc agttcggacc gaatgactac tacatcccga
     2701 agccattagg acccccagca tgaccacgac tattaagttg ctacgatttg gacaaaccca
     2761 catgagatcc tcctggtgca atggtgactg tttgtatcat gaggtaaggg ggaagattag
     2821 tccttcaacc tcctatacgg aatgatttct gaaaaaatcc gtttaagttt tgttcattct
     2881 tccgcaatag acacgtgcaa atccttatcg gttgctttgc agaaaagtta aggatgcccc
     2941 ataacccagg gagtaaggcc ctattgagca ctgtaccggg agcaccacgt atcaggtcca
     3001 cctcaaccac atatataaaa tgggaaccat ccttccctcg ctcgttctga aggaagaaaa
     3061 aaaataaatt ccccggtccg gttggaccgg ggaataggga cagatcctga acgattgaga
     3121 agaaaaaacg ctggaatgag aaattcgggg tcagagcttt gaggctggat tctgtaaaat
     3181 ctccgattga taccagaccc aaaatttacc cact
//
LOCUS       DATIAL010000357         3689 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1140716, whole genome shotgun sequence.
ACCESSION   DATIAL010000357 DATIAL010000000
VERSION     DATIAL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140716"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VLA60_11020"
     CDS             complement(<1..790)
                     /locus_tag="VLA60_11020"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HSF09937.1"
                     /translation="MTPSSFRVLSVIGAGAWGTALAHLLATKGFSIRLWAHEPEVAET
                     IQRTRENSWYLPEVVLPNSIQATISLPDCVQGTDVILLAAPSHAMANMVKQLNLLLKE
                     PLPIIIATKGIEEGTLQLMSQVVESHLPAVWHPFITILSGPSFASEVSRWKPTTILLA
                     GRDFNLVNALQQAFITPQFRVYAGRDMIGAQLGGALKNVMAIGAGVVDGLDLGSNARA
                     ALITRGLAEMIRLGRAMGADVATFYGLSGLGDLVLTCTGTLSRNY"
     gene            1074..1421
                     /locus_tag="VLA60_11025"
     CDS             1074..1421
                     /locus_tag="VLA60_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09938.1"
                     /translation="MSETKPCPSCGYDLPVQGRFCPKCGNRVDRKPVENSKQEPLNLR
                     ILYIMVGLLILAVLFPPWQTPPEQPPEFLGFHFITSPPTEGAQRSPILQNIQLFTIAV
                     AGLYFSWAFRGKG"
     gene            1459..1581
                     /locus_tag="VLA60_11030"
     CDS             1459..1581
                     /locus_tag="VLA60_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09939.1"
                     /translation="MRGTSEDTGHRVYLAPDSPNVPCPIRVSLTIDPGLQRITW"
     gene            complement(1571..2494)
                     /locus_tag="VLA60_11035"
     CDS             complement(1571..2494)
                     /locus_tag="VLA60_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HSF09940.1"
                     /translation="MSQHTGKFVGLMALYVFMGIGAVLFLNGSGFASGEELNTPHSPK
                     KSSESSSPPTHQSPQMDAAKTSLDKKGQEELEALRKKPDEFRTLMTGVQIFLGRFGYG
                     VGPYTGTLDQTTKQALKAYQENSGLAQTGDLDFPTLKRLTEDDRLLNRVVPFLPPQTF
                     HDQEWGQWVEVQGSWMLKEGNTDDVLQTSRITCMKEFKRCIDSTASLVNANVPQLKVH
                     THVYDIQEWDDANIVSAPYDGEACAVSILRISRNPPLATRFLSLQNKPGPCAKVQAED
                     RQYVLEDGPKIYQILRMQKAEAIQQILQVTK"
     gene            complement(3281..>3689)
                     /locus_tag="VLA60_11040"
     CDS             complement(3281..>3689)
                     /locus_tag="VLA60_11040"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HSF09941.1"
                     /translation="IYHHIEKQQNEYLLTYEDGPWYAVWKRSDDRVGTDTPVYSFSSV
                     FAPITLHTTIYHQWEWRPLTKSAQFITTDRIPISITGGREHGYRMYTMKQRLQPGEWR
                     VNVETEDGRLIGRMVFSAEANYSPSHELTTITQ"
BASE COUNT          882 a          873 c         1008 g          926 t
ORIGIN      
        1 ggtaattccg gctcaacgtc ccggtgcagg tgagcaccag atctcctaac cccgatagcc
       61 cataaaatgt ggcgacatcg gcgcccatgg cccggcccaa ccggatcatt tcggccagcc
      121 ctctggtgat taaagcggcc ctcgcgttag atccgaggtc cagcccatcc accacaccag
      181 cgccaatggc catgacattt tttaaggccc cgcccagttg cgcaccgatc atatcccgtc
      241 cggcatacac gcgaaactgt ggagtgatga aggcttgttg tagagcattg accagattga
      301 aatcccgccc ggcgagtaaa atggtcgtgg gtttccaacg gctcacttct gatgcgaagc
      361 tgggcccgga aagaatagtg atgaacggat gccagacggc ggggagatga ctttcgacca
      421 cctgactcat taattgcaat gtgccttctt cgatgccttt ggtggcgatg atgatgggaa
      481 ggggttcctt taataacagg ttgagttgct tcaccatatt ggccatggca tgtgatggcg
      541 ccgcgagcag gatgacatcc gtgccctgca cgcaatccgg aagactgatc gtggcctgga
      601 tggaattggg taagactacc tccgggagat accaggaatt ttctctggtg cgttggatgg
      661 tctccgcgac ctcaggttca tgtgcccata agcggatgct gaatcctttg gtggccagta
      721 ggtgagccaa agccgttccc caggctccgg cgccaatcac agagaggacg cgaaaggaag
      781 agggtgtcac ggtgtctcct ggcaaagatg attcatgaat gatagtggag ttggtgcatg
      841 tgctaaaggg gcaaagcgat gtatcgggag agaaacgtgc accggtctga gggaatgaat
      901 gagggtcgat gttgggattg aatcgtgatc tattgagaaa ttataaaaat gacatgggaa
      961 gtgagtcaaa tcattttggg tgtttcgcct ggacgaatcg atgaaaattg ggcaagatag
     1021 agaaatccaa caatcatgag acatatttta tcttaacaga aatcaggtgc tgtgtgagtg
     1081 aaaccaagcc gtgtccttct tgtggctatg atctccctgt ccaaggacgg ttctgtccaa
     1141 aatgcgggaa tcgggtggat cgaaagccgg tggagaattc caagcaggag ccactcaacc
     1201 ttcgtattct ctacatcatg gtgggccttc tcatcctggc ggtcttattc cctccctggc
     1261 aaacgccacc cgaacagccg ccggaatttt taggatttca ctttattacc tcccccccca
     1321 cggagggggc ccaacgcagc cccatactcc aaaacattca actcttcacg attgccgtgg
     1381 ctgggctgta cttttcctgg gcatttaggg gcaagggcta accagggcat tcctccgagt
     1441 ccggcgagga ggttcttcat gagggggact tctgaggaca cgggacatcg ggtctatctg
     1501 gccccagatt cccccaatgt cccgtgtcct atccgcgtga gcctcacgat tgatcccgga
     1561 ttacagcgga ttacttggtg acttgcagga tttgttgaat agcctcagcc ttttgcatcc
     1621 ggagaatctg gtagattttt ggaccatctt ccagaacgta ttgtcggtct tcggcttgca
     1681 ctttggcgca cggtcctggt ttgttttgca aagaaagaaa acgggtggct agtggaggat
     1741 tccgtgagat ccgtaaaata ctcacggcac aggcttcccc atcatagggt gccgacacga
     1801 tgttggcgtc atcccattcc tgaatgtcat agacatgagt atgcactttg agctggggta
     1861 cattcgcatt caccagggat gcggtcgagt cgatacaccg tttaaattct ttcatgcagg
     1921 tgatgcgcga ggtttgcagg acatcgtcgg tattgccttc cttgagcatc caggaaccct
     1981 ggacttccac ccattgtccc cattcctgat cgtgaaaggt ctgtggggga aggaatggga
     2041 ccacacgatt caagaggcga tcatcctcgg tgagacgttt cagtgtggga aagtcgaggt
     2101 ctccggtctg agcgagccct gaattttcct gataggcttt caaggcctgt ttggttgttt
     2161 ggtcgagagt tcccgtatag ggcccgactc catatccaaa gcgtccgaga aaaatctgca
     2221 ctccggtcat caacgtacga aattcatccg gctttttccg taacgcctcc aattcttcct
     2281 gacccttctt gtctaacgac gtttttgcag cgtccatttg tggggattga tgggttgggg
     2341 gcgatgaaga ttcagaagat tttttgggag agtggggtgt gttcagttct tcgcccgagg
     2401 caaagccgga gccattgaga aagagtactg ccccgatacc catgaataca tacagagcca
     2461 tcaatccgac aaatttaccg gtgtgttgcg acatatcaca ttcccttcta agaaactaaa
     2521 aagcagaaca tctgtgtggt gttcactcaa tacctgtgga gatcaggatc attccaggtg
     2581 gagttcgtgg agtcaagagc tgaagcctgt tcgcccattg agaggtgagc ggtggtccgc
     2641 aaaaaggtga gatggagaaa tgatggaaag aatggagatg ggggagaccg acaaaagcct
     2701 ggtaaagatg gcctctgctc tgattgacgg cagaacagat ggaccatagc aaaacgccgg
     2761 atggagcatg ccccatccgg cgttttgtcc gcacactggg aaaattaccg tttcttcgtg
     2821 gcagcttttt tcttggctgg agcctttttc ttggctggag cctttttctt ggctggagct
     2881 ttcttctttg tggccaaaac actcaccccc tttcaaatga gatgcgacgt cgaagccgca
     2941 gtgatagctc actcctacga tagcagtgag tctgtgaata ggaacacttg aacccgttat
     3001 tagtgtagga cgtcacgcga aaaatttatc ggcgctatcg ttctgcaatg ccaagcgatg
     3061 ttcgggagcg aaattgaaga gggaatgatg taagcaagaa gtgtttcgat agttgtgcat
     3121 gatgatatgg aatctatact acaacgtcta agcaattaat tccaggaatt tttaaatttt
     3181 ttttgtcgtg atgaacccga ggcaaaacgt ctgtcagaaa tcatgtcggc gtgacgaaat
     3241 gacacctgat gaacatgctc gaggaaagag gaaaaatttt tcactgtgtg atggtcgtca
     3301 attcatggga cggcgaataa tttgcttcgg ctgaaaacac catgcgtccg atgagtcgcc
     3361 cgtcttccgt ctccacattc acccgccatt caccgggctg gagccgttgt ttcatggtgt
     3421 acatacgata gccatgttct cttcctccgg tgatggaaat gggaatgcga tccgtggtga
     3481 taaattgagc cgacttggtg agtggtcgcc attcccactg gtgatagatg gtggtatgca
     3541 acgtgatcgg agcaaacacg gatgaaaagc tatagacggg tgtgtccgtt ccgacacggt
     3601 catcggaccg tttccacaca gcgtaccagg gaccatcttc ataggtgagc aggtactcat
     3661 tctgttgctt ttcgatgtga tgatagatg
//
LOCUS       DATIAL010000358         4639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_710861, whole genome shotgun sequence.
ACCESSION   DATIAL010000358 DATIAL010000000
VERSION     DATIAL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710861"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VLA60_11045"
     CDS             <1..755
                     /locus_tag="VLA60_11045"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSF09942.1"
                     /translation="ALLSIVVANVYMNNRNASAFLDLVYAFSPDVVLVTEPDQWWERQ
                     LRSLESSYPYTIKRPQENTYGMLLYSRLKLRNPAVRFLIEDSIPSIFTELELPMGVVI
                     DFYGLHPRPPHVGQDSEERDAELLTVGREVEHTNRPVVVAGDMNDVAWSYTTSLFQRI
                     SRLVDPRLGRGFYNTFHARYPILRFPVDHVFHSSSFRLVELKRLPYFGSDHFPMFAVL
                     SYEPDQPQAQPPAEDGEDREKAQELIKKGKEK"
     gene            complement(960..1391)
                     /locus_tag="VLA60_11050"
     CDS             complement(960..1391)
                     /locus_tag="VLA60_11050"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF09943.1"
                     /translation="MSKTITNNFSARAFFLILCMTCAFAFSACASDSNSRSVGRTVDD
                     AWISSKVKSTLLADTMINGMAVEVETYRGKVMLSGFVDTEDQIQRAVKLTKDIEGVKQ
                     VVNKMVVKSDAIESGTMDQSRTSGSGTFEEPATTPSQSGIQ"
     gene            complement(1978..2295)
                     /locus_tag="VLA60_11055"
     CDS             complement(1978..2295)
                     /locus_tag="VLA60_11055"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HSF09944.1"
                     /translation="MTHPEQFYSLGDDDTIRRHIRIERENGKKLRKTPWWKAQIQKGE
                     CHFCHQQVGAGNLTMDHVVPLARKGKSTRGNVVPACQACNRSKNLTTPVEAILDHLQT
                     KRD"
     gene            complement(2538..3338)
                     /locus_tag="VLA60_11060"
     CDS             complement(2538..3338)
                     /locus_tag="VLA60_11060"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HSF09945.1"
                     /translation="MSRLLMCPPDYFGIEYEINPWMRVSNQTNGERARTQWKTLTQVL
                     ENEVGAKLEFMEPVPKLPDLVFTANAGVIHGGKAVPSQFRHPERQGEERYFIDWFTKK
                     GYEVINLDPGIHFEGAGDLLGFPDFWIGGYRQRSDIRAYVQLSEIFQKEIMPVELVDQ
                     RFYHLDTCFCPLSGGELLYFPPCFDSYAQTVLASRIEQKKRFAVPPREGERFACNAVC
                     IDRHVVLPTGCPETMAWLHKQGYTTHPVELDEFLKAGGSAKCLTLALD"
     gene            complement(3353..4591)
                     /locus_tag="VLA60_11065"
     CDS             complement(3353..4591)
                     /locus_tag="VLA60_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HSF09946.1"
                     /translation="MIVQTVTIQGHIIDSLILAKVLDDIVMLGGTFTLSEVTVGTRRE
                     DTSHATILIEAPTMELLQEILKTIQPHGAVVETEEDCTVEPAPADGILPEDFYATSHL
                     ATQIRWQGNWIDVPQPEMDLAIRLKTSPPSAQMVSMGSVKKGDLVVTGRKGVRVFPLE
                     RPKERDVFGFMEAQVSSERPHRHIIADVAKRMKAIHQQRKEGKGSGKVLFAGGPAIIH
                     AGGREALAWIIEAGYIHVLFCGNALAAHDMEASLYGTSLGYNLGIGRSVPHGHEHHLR
                     TINRVRALGSIQQAVKGGLIKEGIMAACVRQGVQMVLAGTIRDDGPLPDVITDSIKAQ
                     EAMRAAIPGVGLALLVASTLHAVATGNLLPASVPTVCVDINPAVPTKLSDRGSFQAVG
                     LVMDSSSFLWELARELGWKG"
BASE COUNT         1104 a         1165 c         1141 g         1229 t
ORIGIN      
        1 aggccttgct gagtattgtt gtcgccaatg tgtatatgaa caatcggaat gcctcagcct
       61 ttttagacct tgtgtatgca ttctctcctg atgtggtact tgtcacggaa ccggatcaat
      121 ggtgggaaag gcagttaaga agcctggaat cctcctatcc ttacactatc aaaagaccgc
      181 aggaaaacac ctatggcatg ctcctgtatt cacgattaaa actccgcaac cctgcagtcc
      241 gctttttaat tgaagattca atcccttcca tttttacaga actcgaactc ccgatggggg
      301 tagttattga cttctatggg ttgcatcctc gccctcctca tgtggggcag gattccgaag
      361 agcgggatgc ggaattattg actgtggggc gggaggtaga acataccaac agaccagtgg
      421 tggtcgcagg agatatgaat gatgtggcct ggtcgtacac cacaagtttg tttcagagga
      481 ttagcagatt agtggatcca agactaggcc ggggattcta caacaccttc cacgccaggt
      541 atccgatttt gcggtttccc gtggaccacg tgtttcattc ttcctcattt cggttggtgg
      601 agttaaaacg tttaccatat tttggatccg accatttccc catgttcgca gttttatcgt
      661 atgaacccga tcagccccaa gctcaaccac ccgcggagga cggtgaagat cgtgaaaaag
      721 ctcaggagtt aatcaagaaa ggcaaagaga aataacgcgt cactcttttc cctatggatt
      781 gtccgaaaac aataatatta ttgttcggcc agattggtta aagcactggg ccgttttaat
      841 ggatctcttt tttaattgga gtatcaagat gccaaattca cgattgaaga aactacggca
      901 ttaaagtggt attttaggca ccggcccttg gtgactattc gccaagggcc ggtagcattt
      961 tactgtattc cgctttgtga tggagttgtg gccggttcct caaatgtgcc actccccgat
     1021 gtgcggcttt gatccattgt tccgctttct attgcgtcag atttaaccac cattttattg
     1081 actacctgtt taactccttc aatatctttc gtaagtttca ctgccctttg tatttgatct
     1141 tctgtatcca caaatccgct caacattact ttaccccggt acgtctccac ctcaacggcc
     1201 atcccattga tcatagtatc ggccagtaaa gtggatttca cctttgaact gatccaggca
     1261 tcatcaacag ttcttcccac cgatcggcta ttcgaatcac tcgcgcaagc ggaaaatgca
     1321 aaggcacagg tcatacatag gatgagaaaa aaggctctgg ctgaaaaatt atttgtaata
     1381 gtcttggaca taaattcccc ttttgttgat gtttttaaaa gctgccttac ctgcacctcc
     1441 gcttgtattc aaatcgaagg actacaggtt tcttctctgc tattacacgc atctcttcat
     1501 ggcctgaaca ggccatggct aaagcggaag ctaaaaccct cgacgtcaaa ccggccttcc
     1561 gcccttgtta attttgataa cggctatggc gttagcgctc attttccata ggcaaatatc
     1621 gatatcgcgg cgctggcaag ttataagtca gaggtcgttc aggcttccac tccgcacctt
     1681 gcatttgatc gtatcgtgat tcgcccagtg cggttgtaca aagaagtaat ttcaattggg
     1741 taacttcttg cctccgacgc cgcattaaaa cctattatgc ggcaaacccg aaaatgacga
     1801 acccagtaaa aaccctggaa catggatgga aaaattttga atgattaatt ttccgatgaa
     1861 atcgcctggc attcaggcag agggatgtaa ggacaattcc tcattcaggg aaaaaaagca
     1921 aatttccaga tggggtatga gtaaaaaatg cttgaggatt aggacggaaa tgacggttta
     1981 atcccgctta gtttggagat gatcaaggat ggcttcaaca ggggtggtga ggtttttact
     2041 gcggttgcag gcctggcagg caggcacgac attgcctctg gtactttttc ctttcctggc
     2101 caatggcacc acatggtcca tggtcaggtt tcccgctccc acctgctgat gacaaaagtg
     2161 gcactcccct ttttggatct gggctttcca ccatggtgtc ttccgtaatt ttttcccatt
     2221 ctcgcgctca atccgtatat gccgtcggat cgtgtcatcg tcaccgagtg agtaaaactg
     2281 ttctggatgg gtcataaggg aaggtggcct ctgtttacaa tactttatgc ggactggtga
     2341 cgtttgaagt catgagccgg gtcaacctgt tttgaatgat tgtagcgctt gggtcagcgc
     2401 tttttaaaga caaggaggtt ttcatgcctg ggcacagatc cttcgcgggg cctcaggatg
     2461 acaatgattt gaagcgggct cttaccgtca agcggtgttc gtgcagcgtt tgatagggga
     2521 aatcgaattt ttacaactta atccagcgcg agagtcaggc atttggcgga gccaccggct
     2581 ttgaggaatt catccagttc taccgggtgg gtggtgtagc cctgtttgtg taaccatgcc
     2641 atcgtttccg ggcatccggt cggcagcacg acatggcggt cgatacacac cgcattgcag
     2701 gcaaaccgct cgccttcccg gggtgggacg gcaaatcgtt ttttctgttc tatgcgagag
     2761 gccagcacgg tttgggcgta cgaatcgaaa caggggggaa agtagagaag ctctcctcca
     2821 ctgagggggc aaaagcaggt atccagatga taaaaccgtt gatccaccag ttccacaggc
     2881 atgatttctt tctggaaaat ttccgacaat tggacatagg cgcggatatc actgcgttgg
     2941 cggtagccgc caatccagaa atctggaaat cccaataagt ccccggcccc ttcaaagtgt
     3001 atcccggggt caaggttgat gacttcatag cccttcttgg tgaaccaatc aatgaaatat
     3061 cgttcctcac cctgccgttc cggatggcgg aattgactgg gtacagcctt tccgccgtgg
     3121 atgacacccg cattggcggt aaataccagg tctggtagtt ttggtaccgg ttccataaat
     3181 tcgagtttgg ctccaacttc gttctccaat acctgagtca gcgttttcca ttgggtcctg
     3241 gcgcgttctc cattagtttg attggacact ctcatccatg gattaatttc atattcgata
     3301 ccaaaataat ccggtgggca catcaataaa cggctcatgt tattgactcc tgctatcctt
     3361 tccagccaag ttcacgggcc agttcccata aaaaagaaga agaatccatc accagtccga
     3421 cggcttggaa gcttccacgg tcactcagct ttgtgggaac ggcgggattg atgtcgacac
     3481 agacggtagg taccgacgcc ggtagcaaat ttccagtcgc cacggcatgc agggtggagg
     3541 ccaccaacaa ggccaacccc acgccgggaa tggcagcgcg catggcttcc tgggctttaa
     3601 tggaatcggt gatgacgtcc ggcaaagggc cgtcgtcgcg aatcgtcccg gccaatacca
     3661 tctggactcc ctgtcggacg caggccgcca tgatcccttc tttgatgaga ccgcctttga
     3721 cggcttgttg aatgcttccc agggcccgga cccgattgat ggtccgaaga tgatgctcat
     3781 gtccatgggg cactgaacgg ccaataccca aattatatcc cagtgacgtt ccgtacaggc
     3841 tggcttccat gtcatgagcg gccagggcat tcccgcaaaa gagcacatgg atgtatcccg
     3901 cttcgataat ccaggccagg gcttcccgtc ctcccgcatg gatgatggcc ggaccaccgg
     3961 caaacaacac cttccctgac cctttgccct cttttcgttg ttggtggatc gccttcattc
     4021 gcttggcgac atcggcgatg atatgacggt gcggacgttc ggaagaaacc tgggcttcca
     4081 tgaacccgaa cacatcgcgt tcttttggtc gttccagcgg gaatacccga acccccttcc
     4141 gccccgtaac aaccaggtcg ccttttttta ccgagcccat tgaaaccatt tgcgcggaag
     4201 gaggggaggt cttcagcctg atggctaaat ccatttcggg ttgcgggaca tcgatccaat
     4261 ttccttgcca ccggatttga gtggccagat gagaggttgc gtaaaaatcc tccggtaaaa
     4321 ttccatcagc cggggctggc tcaaccgtgc agtcttcctc ggtctcaacc actgccccgt
     4381 ggggctgaat ggttttcagg atttcctgta gtaactccat cgtgggagct tcaatcagga
     4441 tggtggcatg agaagtgtcc tcccgtcggg ttcccaccgt gacttccgag agggtaaagg
     4501 tccctcccaa catgacaatg tcatccagaa ccttcgcgag gatcagtgaa tcaatgatat
     4561 gcccttggat ggtgacggtt tgaacgatca tggtgttatt ttaccatgaa attctgcaaa
     4621 aacttgatgt aaaaatcgc
//
LOCUS       DATIAL010000359         4309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1355889, whole genome shotgun sequence.
ACCESSION   DATIAL010000359 DATIAL010000000
VERSION     DATIAL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355889"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1771)
                     /locus_tag="VLA60_11070"
     CDS             complement(152..1771)
                     /locus_tag="VLA60_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09947.1"
                     /translation="MNPWNTIDSQQLVGLNPWVWVQLESAEPPGPFPFLGGVEPEVVS
                     SLHQVHGIMMSGIETAISDIMAKRASVDDPQLSRRLEDAYAEVVQSRPPLQRHIQCGR
                     KSNGTFQWTYPKNPMASGKMTYGGLRIFNSVSRQAIPFGFERPIGAKVGHFLGFLTGR
                     HTMAEIQTVAQAGGRDLERQLGQFFTLLHDHDCLAAVSNTSIDTHWRQVTRDQEVVHL
                     GHAALLYRHQDDFLWFDPWLMPWFAESPVPSLWTTLLPRPSGIFLTHDHDDHVDPRTL
                     YHLPKDVPVFVPSRRNRKTLHYDYLSLLRELGFSQITELAHGEVWRLGDAHVESVPFF
                     GEDPCDVELPRNCYLIADRGRNTLVHADSGPTNDGRSALHDKVIANLVLKYGPIPLVF
                     ASQQQLKEVRSYAAYACLSPPGKWLEVGENGFLTNAYLAELCHTAQAKCFVSYATGGA
                     DWYPDHLSFMFSGRNPARTALLTANWDPPESLKQELEPFGCRYHYGQALDVFGALPQG
                     ETRISHESDRLAPLGLYQLDHKPPPFLRKHQ"
     gene            complement(1842..2252)
                     /locus_tag="VLA60_11075"
     CDS             complement(1842..2252)
                     /locus_tag="VLA60_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF09948.1"
                     /translation="MQEKSKTRGLRHVALRVRNVRVSQQFYENLFDMKIVWQPDPENV
                     YLSTGYDNLALHQISPEELDRFNLSQIHPLDHLGFLMDSPESVDALYKEATERGITIV
                     KPLKQHRDGSYSCYLSDPDQNTIQVLFEPTVQLQ"
     gene            2527..3390
                     /locus_tag="VLA60_11080"
     CDS             2527..3390
                     /locus_tag="VLA60_11080"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSF09949.1"
                     /translation="MGGTLWNQKVGCVGIGACFLLFITISSKVSATDSSQEIMGKDGA
                     PMVLIPEGPFPMGVPKDARDGGLDERPNHDVFVDTFYMDKYELTNGRYLQFVTETGHR
                     TPQHPTDQKKGLWKGNMMPESVTDLPVINVDWHDANAYCRWAGKRLPTEAEWEKAAKG
                     PNDWRFPWGDVEPTLEHLNFNQSWRGEATLTQVGIYEKGKSPYGVYDVAGNVWEWVAD
                     WYDPAYYAKSPARNPKGPETGTYKTLRSSGWQGETPQVRIFTRIKSLPTDRNNSTGFR
                     CAKDGPDSSTR"
     gene            3417..4286
                     /locus_tag="VLA60_11085"
     CDS             3417..4286
                     /locus_tag="VLA60_11085"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HSF09950.1"
                     /translation="MKHDQSNTGFLGLRVGALESRMATEMERLIIRHGGVPIVAPSMR
                     ELPLSENPQALECGQALLSGQIDMVVLLTGVGLRTLLDVLQTRYTLHEITDALHNSVL
                     VARGPKPAFVLKELGLSPNILVPEPNTWRDTLSALDAAYPDGLHGIRVAVQEYGVSNP
                     EFLEGLRERGAEVQPVPVYRWALPDDLTPLKDLLQQVMDGLVPVLLITNAVQVEHIIK
                     VLGEDEKINHFRKALHRMMVASIGNVASERLRHYGFPVDMEPSHPKMGILVKEASQAV
                     HAILATKNAPPIR"
BASE COUNT         1122 a         1100 c         1084 g         1003 t
ORIGIN      
        1 aggtctctat catgattctc tgtctcccat tgattcagaa caattgttgg gtcccaatca
       61 taccgaactc agagaaagag tccaagacat aaccgttgag ttcaaaaaga tcatttccgg
      121 gaaatcacgt ccttctcgga ctttgccgct tctattgatg ttttcgcaag aagggaggag
      181 gcttatgatc caactgatac aacccaagcg gcgccagccg atccgactca tgcgatatcc
      241 tggtttctcc ttgaggaagg gctccgaaca catccaatgc ctgaccatag tgatagcgac
      301 atccgaatgg ttccaattcc tgcttgagac tttcaggagg atcccaattc gcggtgagca
      361 atgccgtacg ggcaggatta cgcccgctga acataaacga taaatgatcg ggataccagt
      421 ccgcaccacc cgtcgcatag gacacaaagc atttcgcttg agccgtgtgg cacaactctg
      481 caagataggc attggtcaaa aaaccgtttt ctcccacctc taaccatttt ccaggtggag
      541 acagacacgc atacgcggcg taacttcgca cttccttgag ttgctgttga gaggcaaaga
      601 ccagaggtat cggaccatat ttcaacacca gattggcaat gaccttatcg tgcaatgcgg
      661 accgtccatc attggtcggc ccgctatcgg catggaccaa cgtgttccgc ccgcgatccg
      721 caattaaata acagtttctg ggtaattcca catcacacgg gtcttctccg aaaaacggca
      781 ccgactcgac gtgtgcgtca cccaggcgcc agacttcccc atgcgccagt tcagtaatct
      841 gcgagaatcc taattccctg agaagggaca gataatcata atgcagggtt ttccggttgc
      901 gacgactcgg tacgaagacc ggcacatctt tgggcaaatg gtacagggtg cgaggatcga
      961 catgatcgtc atgatcatga gtaagaaaaa ttcctgacgg gcgtggaaga agggtggtcc
     1021 acagggaagg aacgggcgac tccgcgaacc atggcatgag ccaggggtcg aaccataaaa
     1081 agtcatcctg gtgtcgatac agtaaggctg catgacccag atgcacgact tcctgatccc
     1141 gcgtgacctg ccgccaatgg gtatcgatgg atgtgttcga tactgctgcc aaacaatcat
     1201 ggtcgtgtaa cagagtaaaa aactgcccta attgacgctc aaggtctcgt ccaccggctt
     1261 gtgccacagt ctgaatttcc gccattgtat gccgtcccgt gagaaagcca agaaaatgcc
     1321 cgactttggc tccgatcggt cgctcaaacc caaacgggat ggcctgacgc gagacggaat
     1381 tgaagatccg caacccccca tacgtcattt tgcccgaggc catgggattt ttcggataag
     1441 tccattgaaa ggtcccattg gatttccgtc cgcattggat atgtcgttgc aagggaggcc
     1501 gggattgcac cacttctgca taagcatctt ccagccgacg ggagagttga ggatcatcca
     1561 cggaagcccg tttggccatg atgtcgctaa tggccgtctc gatgccgctc atcatgatcc
     1621 catgcacttg atgaagactg ctgactactt ccggttccac tccgcccaaa aaggggaaag
     1681 gccccggggg ttcggcactc tccaactgaa cccagaccca gggattcagc ccgaccagct
     1741 gttgggaatc aatggtattc cagggattca tacatcagtg agattgagat acaacactca
     1801 ggttctgtga aaaataagaa agcccgtgga ttggagctta cctactgaag ttggaccgtg
     1861 ggttcaaaca atacctgaat ggtattctga tccggatccg agagataaca cgaataactt
     1921 ccatcgcgat gctgtttcag gggtttaaca atcgtgatcc ctcgttcggt ggcttcctta
     1981 tacagcgcat ccacactttc cggggaatcc atcaaaaatc ccaaatgatc caacgggtga
     2041 atttgagaaa ggttgaatcg atccagctct tccggagata tttgatgaag cgccagatta
     2101 tcatagcccg tactcaggta gacattttca ggatcgggct gccacacaat tttcatatcg
     2161 aagagatttt cataaaactg ttgtgaaacc cggacattcc gcacgcgaag agccacatgt
     2221 cgaaggccac gggttttgct cttttcctgc atatcctctt cttcagtaat gaagtgatag
     2281 tcctaacctc tttacgcatc ttgtcgttga aaggcatcat acccaggacc ctgagaggga
     2341 gcaagttttt caatcgcagg ttgagagacc gatacaactc cacaggttat tcccttgagg
     2401 atgaaccaaa gagaaggtaa gcgtgtgaat ctttgaccca tcattcagga tgaacggaaa
     2461 ttatggtatt ttatgaatgg gaaaatcaaa gttttgttgc cttgtcatga ataccggagg
     2521 ataaagatgg gtggaacgtt gtggaatcaa aaggttggat gtgtcgggat aggggcatgc
     2581 tttctgctct tcattacaat ttccagtaag gtgagcgcca ccgactcctc tcaagaaatc
     2641 atgggaaagg atggcgcgcc aatggtattg attcccgaag gaccctttcc catgggagtg
     2701 cccaaagatg cccgggatgg aggactggat gaacggccga atcatgatgt gtttgtggac
     2761 accttttata tggacaaata tgaactcacg aatggacggt atctccaatt cgtgaccgaa
     2821 acgggtcacc ggactccgca acatcctact gatcagaaaa agggactgtg gaaaggcaac
     2881 atgatgcccg aatcagtcac cgatcttcca gtcatcaatg ttgattggca tgatgcaaac
     2941 gcctattgtc gctgggcggg aaaacggctt cccaccgaag ccgaatggga aaaggcagca
     3001 aaaggcccaa atgattggcg cttcccttgg ggtgatgttg aacccacatt ggaacattta
     3061 aatttcaatc agtcgtggcg tggagaagcc acattaaccc aggtgggtat ttatgaaaaa
     3121 gggaaaagcc cctatggcgt gtatgatgtg gcgggtaatg tttgggaatg ggtggccgat
     3181 tggtacgatc ccgcctatta cgccaaaagt cctgcccgga atccaaaggg acccgaaacc
     3241 ggcacataca aaaccttgcg aagttccggc tggcagggag aaacgcccca ggtgcgtatc
     3301 tttacgagaa ttaaaagtct gcctaccgac cgcaataatt ccaccggctt ccgatgtgcg
     3361 aaagatggac ctgattcctc tacccgctga ttctttgacc aatccgaaat catctcatga
     3421 aacatgatca atccaatacc ggtttcctag gactccgcgt cggcgccctt gaaagccgga
     3481 tggccacaga gatggagcgg ttaatcatcc gacatggagg cgtgcctatc gtcgctccct
     3541 cgatgagaga acttccactc tcggaaaatc cacaggctct tgaatgtggc caggctttgc
     3601 tatcaggtca aatcgatatg gtcgtcctgc tgaccggcgt gggattgcga acactgctgg
     3661 atgtcctgca gacccggtat actcttcatg aaatcaccga cgccttgcac aactcggtgc
     3721 ttgtcgcgcg aggccccaag cctgcttttg tattaaaaga acttggtctt tctcccaata
     3781 ttctcgtccc ggaacccaat acctggagag atacgctcag tgcgctggat gcggcctatc
     3841 ccgacggtct tcacggtatt cgggttgcag tgcaggaata tggggtcagc aatccggagt
     3901 ttttagaggg gctgagggaa cgtggagcag aagtccaacc ggtcccggtg tatcgatggg
     3961 ccttaccgga tgatctgaca cctttaaagg atttgcttca acaagtcatg gatgggcttg
     4021 tgccggttct cttaatcacc aatgccgttc aggttgaaca catcattaaa gtgctgggag
     4081 aagacgaaaa aatcaatcac ttcagaaagg ccctgcaccg catgatggtg gcctcaatcg
     4141 gcaatgtggc aagtgaacgg ttacgccact atggattccc cgtcgacatg gaaccctccc
     4201 accccaaaat gggcattctg gtcaaagaag cctcccaagc cgttcacgcg atcctggcca
     4261 ccaagaacgc cccgccaatt cggtagtttt tctgtgtcta acccctctc
//
LOCUS       DATIAL010000360         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1248598, whole genome shotgun sequence.
ACCESSION   DATIAL010000360 DATIAL010000000
VERSION     DATIAL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248598"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..253)
                     /locus_tag="VLA60_11090"
     tRNA            complement(164..253)
                     /locus_tag="VLA60_11090"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Ser,seq:cga)
     gene            complement(515..1321)
                     /locus_tag="VLA60_11095"
     CDS             complement(515..1321)
                     /locus_tag="VLA60_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09951.1"
                     /translation="MQSFFQATVRLYHQALLASIQSFTRAWILIPVLLAFALGLVLVT
                     SLIAPLGMIGGFILGALNALVIGSTLSLTEQAVLGARRVSWPDILPSMGQYFWEVISV
                     GFMVWFPLLFLEMGLQTNPYQPLIGTAVFFLIFILLNPVPEVIYQSRNGSALEVVRES
                     YDFVVENWIEWFLPVAIVFGPFGLTFFFEMSSQAGRRGGLDFWHLITLPFSVLSEWLS
                     LLGMSSDMSFVIVLGLTPIVTVFMLFFRGHLFAALHRSSRRQRMFQSRTS"
     gene            complement(1347..1592)
                     /locus_tag="VLA60_11100"
     CDS             complement(1347..1592)
                     /locus_tag="VLA60_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09952.1"
                     /translation="MTLKQLYQDIYQKFLEKKDKYQGYDFQESPVGSATPPSPTFANH
                     VKWWTWNRPSRLKKIRHDRDQRQQDIINIQSPPPSKD"
     gene            2066..2148
                     /locus_tag="VLA60_11105"
     tRNA            2066..2148
                     /locus_tag="VLA60_11105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2100..2102,aa:Leu,seq:tag)
     gene            2202..3527
                     /gene="tig"
                     /locus_tag="VLA60_11110"
     CDS             2202..3527
                     /gene="tig"
                     /locus_tag="VLA60_11110"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HSF09953.1"
                     /translation="MKLEMTELGPVKRSLKIEVPEEAVKSEFQKVYAQLRRQAKIPGF
                     RPGKAPIALLEKRYADLVEQDVVQRLVPDYYQRAVKEAGVVPILVDIPPLERMKVKSN
                     GPFTFTATVEIKPTIQLGDYRPPNPISLKRDARTVTDDQVEKALQSLREQHAELDVVP
                     EGTPLREGLHAVLSLEGFVEDQPLEGTQKKGHLLHIGSKSPILGVELDEYLIGKKAGD
                     MIDIPQPFPANHPDPHLAGKTMVLKVTIDAVKEQKLPELDDEFAKDCGDYDSLEHLKS
                     TINTQLDNFLKQDLEEGYKDQVIERLLQMHHFEIPEVLIERELQTLIRQKMLKDHRHA
                     HREDDVEEPLRLQEEAKRLQQELHPEAKRRVKLSLILDAIVQKEGLHVTEDEVNAEIQ
                     KLATSLKLPVGEIQRMVEAGGQGAQEEFRERMKAEKALQFVYQNAVIQG"
     gene            3609..4238
                     /gene="clpP"
                     /locus_tag="VLA60_11115"
     CDS             3609..4238
                     /gene="clpP"
                     /locus_tag="VLA60_11115"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HSF09954.1"
                     /translation="MLIPMVIEQTNRGERSYDIYSRLLKDRIIFIGAPIDDVFANVVI
                     AQLLFLESEDPEKDIHLYVNSPGGSVTAGMGIYDTMQYVRSPINTICLGQAASMGALL
                     LTAGTKGKRFALPNARVMIHQPMGGFQGQATEIDIHAREILKIREKLNEIMAYHTGQP
                     LDRISQDTERDYFLSGEEAKAYGLIDEVIVRSQEKGHKDDTKAGSKSKD"
BASE COUNT         1148 a          955 c         1182 g         1015 t
ORIGIN      
        1 tgcgatattg tgcccgagac accctggcaa tggtcgagtt gagacgtgcg ttggggaaaa
       61 aggctcggat gaacggccaa taggacgtag acacccagac tttagactgg ccttctatac
      121 cccagcctga taatcaaggc cgccggcaca ggcatgccag gtttggcgga gagggtgggg
      181 atcgaaccca cggtcccgtt gccgggacag cagttttcga gactgcccga ttcggccact
      241 ctcgcacctc tccgcgaatg ggtatctact ttgcggcgac tgaacctctg atgaagccgt
      301 ttgtcttttg gaggggatct gttcatccca tcttagttat cttcttcacc gaggatctgt
      361 ttggtgaaag tttgccatct taccatgggg gtctgggggc gacaagcaag aagggggaat
      421 agttgcgagt agggcgagga agggacaagg agagaacgcc aaccctgttt caggccattc
      481 aatccttagc gttccatgcc ctccctatat catgttagga ggttcgggat tggaacattc
      541 gttggcgtcg ggaagatcga tgcagggcgg caaacaaatg gccacgaaag aagagcatga
      601 aaaccgtcac aatgggagtg agtccgagga cgataacaaa actcatgtcg gaggacatcc
      661 ccaataaaga gagccactca gatagcacgg aaaagggcaa cgtgatgaga tgccagaagt
      721 ccagcccccc ccgccttccc gcctggctgg acatttcaaa gaaaaatgtc aggccgaatg
      781 ggccgaagac gatggccaca ggcaaaaacc attcgatcca attttcaacg acaaaatcat
      841 aactttcccg gacaacctca agggcggatc cgttccggct ctggtaaatt acttccggca
      901 ctggattgag taagatgaaa atcaagaaaa atacggctgt gccaatcagc ggttggtagg
      961 gattggtctg tagccccatt tcgaggaata acaagggaaa ccacaccata aatccaacag
     1021 aaatgacctc ccagaaatat tgccccatac ttggcaggat atccggccaa ctcacacgac
     1081 gggcgccaag gacggcctgt tccgtcaggc tcagggtgga accaatgaca agggcattga
     1141 gggcacccag gataaaccct ccgatcattc caagcggagc gatgagagag gtgacaagca
     1201 cgaggcctaa ggcaaaggcg aggaggaccg gaatcaagat ccacgcgcgt gtgaaggatt
     1261 ggatcgaggc gagcagcgct tgatggtaca gtcgaactgt ggcctggaag aaactctgca
     1321 tgaggcgagg gaatgctccg cagttcttaa tctttgcttg gcggagggga ttgtatgttg
     1381 atgatgtcct gttggcgctg atcccggtca tgtcggattt tttttagccg gctaggacga
     1441 ttccaggtcc accatttgac gtgattcgca aaagtgggcg agggaggggt cgcggaccct
     1501 accggtgact cttgaaagtc atacccttga tatttatcct ttttttccag gaatttctgg
     1561 taaatatctt gatagagctg ttttaaggtc atagagtgtt accttaccca actccctcaa
     1621 gtcgatcaag agggaaaatc cataggaggg ttgagaaaac agatgggtgg tgtttgctcc
     1681 cttcgataat gcgcatgtgg gcattggtct gttctaatct tcaaattgtg ggtatagttt
     1741 ggctggtggg ggtttttcta cacacttcat cccttactca acgagtagca ttatgacttg
     1801 ctgaggagga gatgaagcct ccgaccgggt taaggaggac gattgactcg ttcatcttca
     1861 aacatttccc cccgatcaac ttggattgta gttgcagtgc ttctgggagg actattttcg
     1921 ttaccggaat tttaggatgg cctgaaggag atggacgttt tataataggg aggcttggta
     1981 gggtggagga gttttgttta ccctgaggat tctcggaaga gaacctcttt ttaaagtcga
     2041 tggaatgtgg cgaaaacggt tgcgggcgag agtggcggaa ctggcagacg cgcgagactt
     2101 aggatctcgt gggtaaccgt gggggttcaa gtcccccctc tcgcaccatt tcttaacatt
     2161 tatattcctt attgtgttct tttacaagga tgaatgctct tatgaagtta gaaatgacag
     2221 agctcgggcc ggtgaaacgg tcgttaaaaa ttgaagttcc cgaagaagcc gtcaaaagcg
     2281 aatttcaaaa ggtgtatgcc cagttgaggc gtcaagccaa aattcctgga tttcgacccg
     2341 ggaaagcgcc aatcgcctta ttagaaaaac ggtatgccga tcttgttgag caagatgtgg
     2401 tgcagcggtt ggtgcccgat tattatcaac gagcggtgaa ggaagccggg gtggtcccta
     2461 ttcttgttga tattcctccc ctggaacgaa tgaaagtaaa gtccaatggt ccttttacct
     2521 ttacggctac agtggaaata aagcccacga ttcaattagg ggattatcga ccaccgaatc
     2581 ctatttcttt gaaacgggat gcccgaacgg ttacagatga tcaggttgaa aaggctctcc
     2641 agagcctccg ggagcaacat gccgaacttg acgtggttcc tgaaggaacc cctcttcgtg
     2701 aaggattgca tgcggtgttg tctcttgagg ggtttgtaga ggaccagcca ctggaaggaa
     2761 ctcagaaaaa agggcatctt ctgcacattg ggtccaagtc gccgattctg ggtgtggaat
     2821 tggatgagta tctcatcgga aaaaaggccg gtgacatgat tgatattccg caaccctttc
     2881 ccgccaatca tcctgatcct cacctcgccg gcaagaccat ggtcctcaag gtgacgattg
     2941 atgcggtgaa agagcagaaa ctcccggaac tggatgatga attcgccaag gattgcggcg
     3001 actacgattc actggagcat ctcaaatcca ccatcaatac ccaattggat aatttcctga
     3061 aacaggatct tgaagaaggg tataaagatc aggtgattga acgtttgttg cagatgcacc
     3121 actttgaaat tcccgaggtc ctgattgaac gggaacttca gacgctcatt cgacaaaaaa
     3181 tgctgaagga tcatcgccat gcccatcgtg aagatgacgt ggaagagccc ttacgattac
     3241 aagaagaggc caagcgcctc caacaagaat tgcatccgga agctaagcgg cgtgtgaagc
     3301 tgagtttaat tctggatgcg atcgttcaga aagaaggcct tcatgtcacc gaggatgagg
     3361 tgaacgccga aattcaaaaa ctggccacct ctttaaagct cccggttggt gaaattcaac
     3421 gcatggtcga agccggtggg cagggtgcgc aggaggagtt ccgcgagcgg atgaaggcgg
     3481 aaaaagcctt gcagtttgtc tatcaaaatg cggtcattca ggggtaacgg gtcgtacaac
     3541 ccttctgaat gcacctgttc tgatatctgt atttgatacc tatttacaac acgaggaggt
     3601 ctcagggaat gctcattccg atggttattg aacaaacgaa ccgaggtgaa cggtcatatg
     3661 atatttactc acgtcttttg aaagaccgga tcatctttat cggagccccg attgatgatg
     3721 tgttcgcgaa tgtcgtcatt gcacagctgt tgttcttgga atccgaagac ccggaaaagg
     3781 acattcattt gtatgtcaat tcgcctggcg gaagtgtgac ggcgggaatg ggaatctatg
     3841 ataccatgca atatgtgagg tcgcccatca acaccatctg tttagggcag gcggcaagta
     3901 tgggagccct gttattaacc gcagggacta aaggtaagcg ttttgctctt cccaatgcac
     3961 gcgtgatgat tcatcaacct atgggaggat ttcagggcca agcgacggag attgatattc
     4021 atgcccgcga gattttaaaa attcgcgaaa aattgaatga aattatggcc tatcacacag
     4081 ggcagcctct ggatagaatc tctcaagata ccgaacggga ttatttttta tcgggggaag
     4141 aagccaaagc gtacggatta attgatgagg tcatcgtccg aagtcaggaa aaggggcaca
     4201 aggacgatac caaggcaggt tccaaaagta aggattaagg gcaaaggagg catttcttat
     4261 ggcgagacaa gcgaagtccg aagccaatct tcgttgctcc
//
LOCUS       DATIAL010000361         4467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1464879, whole genome shotgun sequence.
ACCESSION   DATIAL010000361 DATIAL010000000
VERSION     DATIAL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464879"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..828
                     /locus_tag="VLA60_11120"
     CDS             151..828
                     /locus_tag="VLA60_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSF09955.1"
                     /translation="MNTLRASLRRYFLTGLLVITPIWGTILILKTLFVTVDSILGTAL
                     ADLVLPDYYFPGLGILALLLLIFSAGVFATNFLGKRLVVRWEEILDRVPIVRGIYATL
                     KSMMDILSFKQREKYNKVVMIQFPKEGNYCLSFVTGETRKEIQNLAPEPLVHVFVPTS
                     PNPTSGYFLLVPESEIIPVDMGVEEAMKLIVSGGFYSPPDKSSSIEIAQKRDRMTLKD
                     SPKVQTP"
     gene            863..2374
                     /gene="glmU"
                     /locus_tag="VLA60_11125"
     CDS             863..2374
                     /gene="glmU"
                     /locus_tag="VLA60_11125"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HSF09956.1"
                     /translation="MSGKKNTAAMILAAGQGTRMKSALPKVLHRVAGRPMLWYAANLA
                     RQVATQTVAIVVGHGSEQVQAYLEQEKANFEPFEVVIQEKQLGTGHAVQQAYPVCTRN
                     DAKQADQFLILNGDSPLLTQATLTSLVDYHQSEKAGLTILTTVIPESRGYGRVVRGPS
                     GEIRRVVEDQDCSAEERSISEINVGTYVVDGTFLGKALSQLRPQNAQGEYYITDMIEV
                     AVQEGLKVVAWITNDHLETTGINTREHLAIAEKEMRRRICQRLMLSGVTMLDPERVII
                     DDGVEVGRDTSLYPGVILEGRTVLGANCVIHANSRLNNCLVGNNVLIQDSCVLLEARI
                     EEGAVIGPFAHLRPGSHIHRKGKVGNFVELKQTEVGEGSKVNHLSYLGDTVIGRNVNI
                     GAGTITCNYDGFRKARTQIEDNVFIGSDVQLIAPVTIGEGALIAAGTTVTKNVPPNAL
                     GISRVAQVNKEGTAAKRREILASSSAAHIQDKEHGDPAESSLRPNPQQQKDPV"
     gene            2502..4331
                     /gene="glmS"
                     /locus_tag="VLA60_11130"
     CDS             2502..4331
                     /gene="glmS"
                     /locus_tag="VLA60_11130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HSF09957.1"
                     /translation="MCGIIGYVGGESATPILVAGLQRLEYRGYDSAGIAIQHNGELKI
                     RRTVGKLTNLQALLQNGAVAGTIGIGHTRWATHGRPSEQNAHPHRSGEIVLVHNGIIE
                     NFLDLRHRLELEGYRFESETDTEVIAHLLSSLTIKGHGLQEAVRLIANELHGSYAIAV
                     MSLAEPGRIVVSRSGCPLVIGQNGKGAFLASDVTPLLAHIREVVFLEDGDVGILEGSG
                     ITIFDSDGQPKGFNPVTIDWDAEAAEKGGYPDFMMKEIHEQPQVIMDTLRGRFQYETG
                     EADLPDLAMTDEELLNARRIWIVACGTSWHSGLVGKYLFEEMIRRPVQVDIGSEFRYR
                     EPMIQKDDLFIAISQSGETADTLAAAREAKHRGARVLAIVNVVGSTLAREADGVIYTR
                     CGPEISVASTKAFTGQVIALYLLALHVGRVRRTLSPEEGRWWLDHFLKLPGQVEHILK
                     QEASIQAIAQRYYQKRNFLYLGRGINYPIALEGALKLKEISYIHAEGYAAGEMKHGPI
                     ALIDEDMPVVVLSPKDRLYEKSVSNLMEVRARSAPVIAFVTEGDHALDLVADHVFAIP
                     AVHALLTPILFTVGLQLLAYHIAVLRGVDIDRPRNLAKSVTVE"
BASE COUNT         1084 a          995 c         1254 g         1134 t
ORIGIN      
        1 cgatcctagt ctaagtcatt gaaattcgca agaaaagtct tgcaggagtc ccatcctgat
       61 tgtaagatgc ggtagtatta cggtgacttc ctacctctcc cgtatagacc gtgcctgctc
      121 tgtgtggcgg gtaaaaggcg gatttttctc atgaacaccc ttcgcgcctc tctcagacgt
      181 tattttttga caggcctgtt ggtaattacc ccaatttggg gaacgatcct cattttaaaa
      241 acgctgtttg tgaccgtgga tagtatcctt ggcacggcat tagcggacct ggtgttgccg
      301 gattattact tccccggtct gggtattttg gcattattgc tattgatttt ttcagccgga
      361 gtctttgcca caaatttttt ggggaaacgt ttggtcgtcc ggtgggaaga aattttagat
      421 cgcgttccga ttgtacgtgg tatttatgcc acattgaagt cgatgatgga catcttatca
      481 ttcaaacaac gcgaaaaata taacaaagtg gttatgattc aatttcccaa agaggggaac
      541 tattgtttgt cgtttgtcac gggggaaacc agaaaggaaa tccagaatct ggctccggag
      601 cctctggtcc atgtgtttgt ccccacatcg ccgaatccaa cttctggcta ttttctcctg
      661 gttcccgaga gtgaaattat tccggtagat atgggggttg aggaagccat gaagcttatt
      721 gtttcaggcg ggttctattc tcctccggat aaatcctcta gcattgaaat tgcacaaaaa
      781 cgagatagaa tgaccctcaa ggactcacca aaagtccaaa ccccatgaga agagaccttt
      841 aacgaagggt atgacaggcg taatgagtgg taaaaaaaac acggccgcca tgattctggc
      901 tgcgggacaa ggaacccgga tgaaatcggc tttgcccaaa gtcctgcatc gagtggccgg
      961 tcgtcccatg ctgtggtatg ccgcgaactt agccaggcaa gtggcaaccc agaccgttgc
     1021 cattgtggtc ggccacggat cggaacaggt tcaggcctac ctcgaacaag aaaaggctaa
     1081 ttttgagccg tttgaagtgg tgattcagga gaaacaattg gggacggggc atgcggttca
     1141 acaggcgtat ccagtatgta ccaggaacga cgccaagcag gccgaccaat tcttgattct
     1201 caatggcgat tcacctttat taacgcaggc cactctgact tccctggttg attaccatca
     1261 atctgaaaaa gccggcctga ccatcttaac gacggtcatt ccagaatcac gcggatatgg
     1321 gcgggtggtt cgcgggccat caggcgagat ccgtcgtgtt gtcgaggatc aagattgctc
     1381 agcagaagaa cgaagtattt cggaaattaa tgttgggacg tatgtcgtgg atggcacctt
     1441 ccttggaaag gcgttgagcc aattgcgtcc gcaaaatgcc caaggggaat attacatcac
     1501 cgatatgatt gaagtggctg tccaggaagg cctgaaagtt gtggcatgga taaccaatga
     1561 ccatttggag accactggga ttaacacaag ggagcatctg gctatagccg aaaaagaaat
     1621 gagacgacga atttgccagc gtctcatgtt gtcaggtgtc accatgctgg atccggaacg
     1681 tgtgattatt gacgatggag tcgaggtcgg aagggatacc agtctgtatc ccggagtcat
     1741 acttgaaggc cggaccgtcc ttggcgccaa ttgcgttatt cacgccaatt cgaggctgaa
     1801 caattgcctg gtcggcaata atgtcctcat tcaggattcg tgcgtcttgc tggaagccag
     1861 gatagaagag ggggcggtca tcgggccttt tgcgcatttg cgtccgggga gccatattca
     1921 tcgaaaaggc aaggttggta attttgtgga attgaaacag acggaagttg gagaggggtc
     1981 taaggtcaat catttgagtt atctcggaga taccgtgatc ggccggaatg tgaatatcgg
     2041 cgccgggacc attacctgca attacgatgg atttcgaaag gcacggacac agattgagga
     2101 caatgtgttt attgggagcg atgtgcaatt aattgccccg gtgacgatag gggaaggggc
     2161 gttgattgcg gcagggacca ccgtcacgaa aaatgttccg cccaatgcat tgggcatttc
     2221 tcgagtcgcg caagtcaata aagaaggtac ggcggcgaaa cggcgagaaa ttctggcttc
     2281 ttcctccgcc gcccatattc aggacaaaga gcatggtgac ccggcagagt cgtcactccg
     2341 accgaaccct cagcaacaaa aagaccctgt ctagtggtca attttgatga acgaagtcga
     2401 gcgatatttg gccgggctca tgtgttttta ggctgagcga taccatcccg atctttccaa
     2461 taccatttgg aaaaatgatg cgatgtaagg agcgttagag tatgtgtgga atcatcggat
     2521 atgttggagg tgaatcagcc acccctattt tagtggccgg gctacaacgg ctggaatatc
     2581 gagggtatga ctccgccggg attgcgattc aacataatgg tgagttgaaa attcgaagga
     2641 cggtgggcaa gctcaccaat cttcaggccc ttttgcaaaa cggggccgtc gccggcacaa
     2701 taggaatcgg tcatacgcgt tgggccacgc atggtcggcc atctgaacaa aatgcccatc
     2761 ctcaccgttc gggagagatt gttctcgtcc ataacggaat cattgaaaat tttctagatc
     2821 tccgccatcg attggaatta gaaggctatc ggtttgagtc tgaaaccgac accgaggtga
     2881 ttgcgcacct cttatcctcg ctcacgataa aagggcatgg gcttcaagag gcagtcagat
     2941 taattgccaa tgagcttcat gggagttatg ccattgccgt gatgtcgctg gctgagccag
     3001 gacgcatcgt cgtcagtcgt tcaggatgtc ccctggtgat tggtcagaat ggaaaaggag
     3061 cctttttggc ctctgatgtc acgcctctcc tggctcatat ccgggaggtg gtgtttctcg
     3121 aagacggaga tgtgggcatt ttagagggtt ccggtatcac catttttgat tcggatggcc
     3181 aaccgaaagg cttcaatcct gttaccattg attgggatgc tgaggcggct gaaaaggggg
     3241 gctatccgga tttcatgatg aaggaaatcc acgaacaacc acaagtgatc atggatacgc
     3301 ttcggggccg gtttcaatat gaaacgggcg aagcggatct tccggatttg gccatgactg
     3361 acgaggagtt gctcaacgcc aggcgaatct ggattgttgc gtgtgggacg tcttggcact
     3421 cggggctcgt cgggaaatat ttatttgaag aaatgattcg acgccccgtt caggtggata
     3481 ttggttccga gttccggtac cgggagccta tgattcaaaa ggacgatttg tttattgcca
     3541 tttcacaatc aggcgagacg gcggacacat tggctgcagc acgggaagcc aagcaccggg
     3601 gggcccgtgt cctggcgatt gtgaatgtgg tgggaagtac gctggctcgc gaagctgatg
     3661 gggtgattta cacgagatgt ggtcctgaga ttagtgtggc ttccacgaaa gcctttaccg
     3721 ggcaggtaat agccttatat ctcttggcct tgcatgtggg acgagtgcgt cgcacgttaa
     3781 gtccggagga agggcgatgg tggttggatc attttctcaa gcttcccggc caagtcgagc
     3841 atattcttaa gcaggaagcg tcgattcagg cgattgccca gcggtattat caaaaacgaa
     3901 attttttgta tttgggtcgg gggattaatt atcccattgc cctggaaggt gccttgaaac
     3961 tcaaagaaat ctcctatatc catgccgagg gatatgcggc gggggagatg aagcatggtc
     4021 ccatcgcatt gattgatgaa gacatgcctg tagtcgtgct atcgccgaaa gatcggctct
     4081 acgaaaagtc tgtcagtaat ctgatggaag tgcgcgccag gagtgccccc gtgattgcat
     4141 ttgtgaccga aggggatcat gccttggatc tggttgccga tcacgtgttt gcgattcctg
     4201 ctgtccatgc gctcttgact ccgattctgt ttaccgtcgg tcttcaactg ctggcctatc
     4261 acattgcggt gttgcgaggg gtagatattg accgtccgag aaatcttgcc aagagtgtga
     4321 cggttgagta aggcgtcctt ccgacctccc accagagtct ttcctgcttg gttctcacgg
     4381 cttctatcaa gtggagagta ttgaatcatg gagaccatga ttgtgaccgg aggcgctggt
     4441 tttattggcg ccaactttgt ccggatg
//
LOCUS       DATIAL010000362         8477 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1356142, whole genome shotgun sequence.
ACCESSION   DATIAL010000362 DATIAL010000000
VERSION     DATIAL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356142"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..1119)
                     /locus_tag="VLA60_11135"
     CDS             complement(265..1119)
                     /locus_tag="VLA60_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09958.1"
                     /translation="MPYTLKIFASYLKVCACCMVMMVIAAFVHSPADAAPIAVRFSEG
                     VTHGFLLVRSLAGEIIGQGEMTQVVQEGDLVESHLVFKFKDGSLHDEKVAFSQQHVFT
                     LISYRLIQHGPSFPEQIDVAIDRGTGEYTVRSQEGESGKEEVLTGTFDLPKDVYNGMV
                     VTMVLNLPKGAGETVHMLAFTPQPEVIKLELRPMGEHTVHIGDLSRKALQYVFKPDIG
                     LIRKWLGRITGQLPGKFHYRCWILADEVPSFVHYEGPLQLMGPIVRIELVSPHLLTKP
                     EDKKTPSK"
     gene            complement(1562..1711)
                     /locus_tag="VLA60_11140"
     CDS             complement(1562..1711)
                     /locus_tag="VLA60_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09959.1"
                     /translation="MDKRQGVAIQSVLETGTVKKILAENGHVPKEHGAVGGPFLPVKK
                     ITLYM"
     gene            complement(1853..2152)
                     /locus_tag="VLA60_11145"
     CDS             complement(1853..2152)
                     /locus_tag="VLA60_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09960.1"
                     /translation="MTNEEKHKAIRQWIDPEERVTVHYLDAPDLNAEVTGCTDQLVDL
                     SIETHVSHMKQRISVPLSQVEVSEDFSHYTRDPERPLRPSRLMLVIDEKRPPIIY"
     gene            complement(2707..2850)
                     /locus_tag="VLA60_11150"
     CDS             complement(2707..2850)
                     /locus_tag="VLA60_11150"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HSF09961.1"
                     /translation="MSTLVIIVLVLLLVGVLPTWPHSANWGYTPSGGVGLVLQVLVLT
                     SRI"
     gene            complement(2998..3210)
                     /locus_tag="VLA60_11155"
     CDS             complement(2998..3210)
                     /locus_tag="VLA60_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09962.1"
                     /translation="MTCRVARLFQQSTQIKPVACCKCGTRDLDVFFTSKALGLHLCST
                     CAQSMIVGEVGKELSARAKQEEPEQH"
     gene            complement(3500..4153)
                     /locus_tag="VLA60_11160"
     CDS             complement(3500..4153)
                     /locus_tag="VLA60_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSF09963.1"
                     /translation="MEGKSELSFNLQKFLAQDGSGKKILTFHEKENLFSQGDVADAVF
                     YIQSGQVKLSVVSKQGKEAIVGIVEQGGFLGEGCLVGQRVCMATATALEDSTLVRIDK
                     RTMIQVLHEEPTFSTVFMEYLLARNIRIQEDLVDQLFNSAEKRLARVLLLMANFGKEG
                     KPEPVLAKISQETLAEMIGTTRSRVSYFMNKFRKLGFIEYNGGLYVHSSLLNVVLHD"
     gene            4831..5253
                     /locus_tag="VLA60_11165"
     CDS             4831..5253
                     /locus_tag="VLA60_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HSF09964.1"
                     /translation="MALLCLSVLLTGCQDPPIHELQKARQAVEHARREGALNFAPDLY
                     SLAESELTIGEEEFHEQSRKMFWARDYSMATRLIMLAQTDAQQALSLAQEEKQKSSMS
                     DRDPSPLLSVHTYLEELRLTNGGLFFQGRERRNGPQAP"
     gene            5527..6390
                     /locus_tag="VLA60_11170"
     CDS             5527..6390
                     /locus_tag="VLA60_11170"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF09965.1"
                     /translation="MEPIVGTTGDMVIRYGLSTFFLFLIILIVRMGIHHSLFRSTELS
                     VETRRRWAVNLRNGLLFLFILGMIFIWAPQLQTFAVSVFAVAVAFVLATKEIIDCLSG
                     SGLRVLTKAFSLGDRIEIGGIRGNVVDHNALTTTVLEIGPGQTSHQYTGRAMVIPNSF
                     IFDHPLINETYTKKYRLHIVTVPLSTDDDWKTAERLLLQAGEEEAAPFIDEARTYLKK
                     LEGKLWLDAPSVEPRVTIQLPEPGRINLLLRVPCPTQYPSRLEQAILRRFLAEFSFAP
                     RLVIQESHSSH"
     gene            6575..>8477
                     /gene="treS"
                     /locus_tag="VLA60_11175"
     CDS             6575..>8477
                     /gene="treS"
                     /locus_tag="VLA60_11175"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HSF09966.1"
                     /translation="MIVDILENDPLWYKDAIIYELHVRAFCDSNADGIGDFKGLTQKL
                     DYLQDLGVTAIWLLPFCSSPLRDDGYDISDYTNIHPHYGTRRDFQAFVREAHRRGLRV
                     ITELVVNHTSDQHPWFQRARKAPPGSKWRNWYVWSETPDLYSDTRIIFKDTEHSNWTW
                     DPVAKAYFWHRFFSHQPDLNYDNPEVRKAIFEVLDFWLELGVDGLRLDAVPYLFEREG
                     TNCENLPETHAFLKTLRTHVDKKFKNRFFLAEANQWPEDAAAYFGEGDECHMNFHFPL
                     MPRLFMSMQMEDRFPIIDILDQTPAIPEACQWGLFLRNHDELTLEMVTDEERDYMYRV
                     FAHDKQARINLGIRRRLTPLLGNDRKKIELMYSLLFSMPGTPVIYYGEEIGMGDNFYL
                     GDRNGVRTPMQWSADRNAGFSYGNPQKLYLPIIIDPEYHYEAVNVELQQNNAQSPLWW
                     MKRIVSLRKRYKVFGRGSIEFLHPSNRKVLVFLRRYQDETILVAVNLSRHAQWVELDL
                     AEFKGRRPMTLFGRSKFPAIGDLPYLLTLSGHAFYWFALEPVESKQLEAQGKTEHGLP
                     TITISKDWDNLIQKREKVKLENVLPQYLQGRRWFGGKARTMQFVEVTETIPLPQENPL
                     AVLALIRVEY"
BASE COUNT         2097 a         2272 c         1963 g         2145 t
ORIGIN      
        1 cctttcctcc tggccatcga ggacgaacct cacaaagtgg gctgtatcat gcctagaaga
       61 ctgatctcgg tctgatcatt aatgctcagc tttgaattga tacagtagcg gcttgactct
      121 gacttgctta caaacaggcc ataagcatgg cgatctgaag aaggctttgt cgccgacagg
      181 tcatacctta ttcttaacta tctatccaac tagacgcatg gtagtctggc caaaatcgct
      241 cactcttgaa atgactaaac ctcgttattt tgaaggtgtc tttttatctt cgggcttggt
      301 cagcaggtgg ggactcacca gttcgattcg cacgatcggc cccatgagtt gcaagggccc
      361 ttcatagtgc acgaaacttg gaacctcatc ggccaggatc caacagcgat agtgaaattt
      421 ccccggcagc tggccggtaa ttctgccgag ccacttccgg atcaggccga tgtccggctt
      481 gaacacgtac tgcagagcct tccgtgacag gtcgcctatg tggacggtgt gttcacccat
      541 gggacgaagt tccagtttga taacctccgg ttggggcgtg aaggcgagca tgtgcaccgt
      601 ttcgccagcc cctttgggca gattcagcac catcgtgacc accattccat tgtagacatc
      661 cttgggcaga tcaaacgttc cggttagcac ctcttccttc ccactctccc cttcttgtga
      721 tcgaaccgtg tattctcccg tgccccggtc tatcgcaacg tcgatctgct ccggaaacga
      781 cggaccgtgt tgaatcaggc gatagctgat caaggtaaac acatgttgtt gggaaaaggc
      841 gactttttca tcgtgcagag agccgtcctt gaacttgaac accaaatgac tttcgaccag
      901 atctccctct tggacaacct gagtcatttc accctgacca atgatctccc cggccagcga
      961 acgcacgagc agaaagccat gcgtgacccc ttccgaaaat cggacggcaa ttggagcagc
     1021 gtccgccggt gagtgcacga aggccgcgat caccatcatg accatgcaac aggcgcagac
     1081 cttcagatag gatgcaaaga tttttagagt atagggcatg atgcggaata ctttctttct
     1141 tgtttcagcc actcacttag ggctggaatt ctgctctatg attaattaag agacaactct
     1201 acctaatgcc aatgatggaa aaaggaagca acaaacccac gctccttttg tagccataca
     1261 cagaagaccc aagcgtcaaa accgctattg cattcatttt ctccatattg tgggtcttcc
     1321 ggattgtggc aaatatgcca acaaggctgg cgatcgtcaa ggagttgccg acatcactac
     1381 aatttcccag aaagactcga actaaaaaga tcctgacatt ccggtagaag ccacgatgca
     1441 tccctacccc aaatgagcaa tgacatagag gaagcctgaa aggagcctcc atgggccgct
     1501 agtagcaaaa cgtcccagcg aaacgttttt ctggtctgag gcgccaggag taggttggcc
     1561 atcacatgta caacgtaatt ttcttgaccg gcaaaaatgg gccgccaacg gctccatgtt
     1621 cctttggaac gtgtccattc tctgctaaaa tcttcttcac cgttcccgtc tcaagaacac
     1681 tctgaatcgc cacaccttga cgcttatcca tgagaccgga ctatccacta cggcattcca
     1741 caatcgattt ccagctaata tggtcgtgtt ttcattcatg cctttaccag ctacgtgtca
     1801 tttaatttga cccaaaaagt cgagagtctc attcgcatat tcccttattc gcctaataaa
     1861 taataggtgg gcgtttctca tcaataacca gcatcaatcg actgggccga aggggtcgct
     1921 cgggatctct tgtatagtgc gagaagtcct ccgacacctc aacctggctg agcgggacgg
     1981 aaatacgttg tttcatatgg gacacgtgag tttcaatcga gaggtcgacc aattgatcgg
     2041 tgcaacccgt gacttcagcg ttcaggtccg gcgcatcaag gtaatgcacc gtgacacgtt
     2101 cttccggatc aatccattga cgaatggctt tgtgtttttc ctcgtttgtc atgcgtgctc
     2161 cttctttcga gtcgttaaag agaaatccag gcaggacagc caacgagaaa ggagggggat
     2221 cgcacctact cggctgttca attcatcggg gtctatgcca cggctgttga atttcatgaa
     2281 tgagccctgg aggtgctcag tagcgacatc aacacgacgg atgttgtttt ttatactcta
     2341 aagtcatcac acttttctaa atgtgtttcc agatccaagt ccagaatttt gggtatcagc
     2401 catggactca cgcgactgca tggttaaatt ttcccctgct ggcactaccc taaccatagg
     2461 aacacgatgc cgaaaatcca tgagtctcta cttcatcgca acataattaa ttaacaattc
     2521 acaccaccac cgaacacacc agaaaataat tcaaatcaga tcatatcgcc tcctgattcc
     2581 tggtgataat acccgcttga tacatttttt caatttaacg attgttgcaa aagaatcttt
     2641 ttgattctcc attacttcat tgctgttgtt cccaatttcc cgtctctcac ccttgtgtac
     2701 cggccatcag attcggctgg tcaaaaccag gacttggagg accaagccta ccccgccgct
     2761 gggggtataa ccccagttcg cgctgtgtgg ccatgtgggc agtacgccaa cgagtaacaa
     2821 aaccagcaca atgatcacaa gggtactcat gactttttcc tcattttttc agcttttcgg
     2881 ctaggacaat cgcccctccg cctgactgtt ccgatttgga catccttccc atccctgacc
     2941 aacgcgaaca aatcggatga gattttcctg tcttggctga ggtggatgag gtttcgctca
     3001 gtgttgctcc ggctcctcct gctttgccct agcgctcaat tccttcccca cttcgcccac
     3061 aatcatggac tgggcacagg tcgagcacag gtgtagtccc aaggccttcg aggtaaagaa
     3121 cacatccaga tcccttgttc cacacttaca acaggcgact ggctttattt gggttgattg
     3181 ttgaaacaac cgtgcgactc ggcaggtcat actgttacct ccatactcgc tcagaatatt
     3241 ggcatggaga acggcagaat gtagattgag tttagcggga ggacagcttg tagtctgttc
     3301 aaaattgctc agtaaaaaaa gatttggaaa ggtagcggag tggacctcat ctagccctcc
     3361 accactgcac cgacacagcg gaaatggagg gaatttagga gcctgtaacg tttggttata
     3421 gactatatac gctaatgaga aaatcgattg gacagcttaa tttcgtcgag tccagtatat
     3481 aatcacttgt tacagcccac tagtcgtgaa ggacgacatt caggagagag ctgtggacat
     3541 acaacccccc gttgtactcg atgaagccca atttcctgaa cttattcatg aaataactga
     3601 cccgggaccg tgtggtcccg atcatctcgg caagcgtttc ctgactgatc tttgcaagga
     3661 ctggttccgg tttgccctct ttcccaaaat tggccattaa gagcagcacc cgcgcgagcc
     3721 gcttctcagc cgaattaaac agctgatcca ccaaatcctc ttgaatgcga atgttgcgag
     3781 ccagaagata ttccatgaac accgtcgaga aggtgggttc ctcgtggagc acctggatca
     3841 tcgtccgctt gtcaattcgt accagagtgg aatcttccag ggctgtcgcc gtggccatgc
     3901 acacccgctg cccgacgaga cagccttccc ctaaaaaccc cccttgctcc acaatcccga
     3961 cgatggcttc tttgccctgc ttcgagacca ccgacagttt gacctggcct gactgaatat
     4021 agaacacggc atccgccaca tcaccttgcg aaaaaagatt ctccttttca tgaaacgtca
     4081 ggattttttt tccactaccg tcttgagcca gaaacttctg gaggttaaag gacaattccg
     4141 acttgccttc cataggcttt ctcctttgag agatctggat aggatctgga taggatctgg
     4201 ataaaaattc tgaacaaaga gaggggattt tttgcacaaa ccttgtcaat gcggaatatt
     4261 ttcccataag tgcaatcatc tgatcaagaa tgttactgaa gggatgcctc acgagtatct
     4321 cttgggttga tctcgtgaat gatgatcagg caaatctgat aggcgacctt tccccgcacc
     4381 cccgccatct ttggaggcca tttcaccctc gataggaaat tccaagccat acgcatagcc
     4441 tcggacatcc cggggcacct taaccttctt ctcgcttatc gtctccagga caaacggtca
     4501 atccccggtg aaggatcacc accacaccct ttgggtcctt ctttttccaa aaccagaccg
     4561 ggacaaaggg tccaaccatg agccaaactg tcccgcccac cgggaccctt tcatgaaaaa
     4621 tagttgaaaa atccggtatc taccaacacc ctttcggcat tcggtattgg cgtaggattt
     4681 gctttatcag ccagtgaatc tgaggaaaat cactcacggc cttccccccc aggaaccgtc
     4741 caaaagcaag gtcccatttt gcgagaagag aaactgccca tatggagatt cgttgagagg
     4801 actcagaggc gttcccctgg gggaatatat atggccttgc tctgcttgag tgtcctgttg
     4861 accggctgtc aggatccgcc gattcacgaa ttacagaaag cccgtcaagc agtggaacat
     4921 gccaggaggg aaggagcctt gaacttcgca cctgatctct acagtttagc ggaaagtgag
     4981 ctgacgatcg gagaagaaga atttcatgaa caatccagaa aaatgttctg ggctcgagat
     5041 tattctatgg ccacccgatt aatcatgttg gcacaaacgg atgcccagca agccctctct
     5101 ctggcccaag aagaaaaaca gaaatcttcg atgtcagaca gagatccttc ccctcttctt
     5161 tcagtccaca cctacctcga ggagcttcgt ctgaccaacg ggggcctttt cttccagggc
     5221 cgcgaaagga gaaatggtcc tcaagctccg tgactcctgc caccctcttt gattaggaga
     5281 acaaatacag tatgatttga gaaggttggt tgtcatttct gaacatgatc tgtcccctga
     5341 cggcttttgt tcattcgctt tcaccccgaa ccgttggcat gtgatttttt ttctcgttca
     5401 tctgtctatt cctggctata cgccttttcc ccaatcggtc agattcggaa tgatggctga
     5461 gacgctcatg gctctcctgt tgatctcaac gcgtccaatt cacccatctg tcatgaggag
     5521 ttacacatgg aacctattgt aggtacaacc ggcgatatgg tcatccggta cggcctgagt
     5581 acgttctttc tcttcttaat tattctgatc gtcaggatgg ggatccacca cagtctcttc
     5641 cggtcaaccg agctttccgt ggaaacacgg aggcggtggg cggtcaacct cagaaatggc
     5701 ctgctctttc tattcatcct cggaatgatt ttcatttggg cgccgcaact gcaaacgttt
     5761 gccgtctcgg tatttgctgt cgccgtcgcc tttgtcctag ccaccaagga aatcattgac
     5821 tgcctgagcg gatcagggct ccgggtgctg accaaagcct tttcgttggg ggatcgcatc
     5881 gaaatcggag gcattcgggg aaacgtggtc gaccacaacg ccctgaccac cacggtattg
     5941 gaaatcggac ccggccagac gtctcaccaa tataccggcc gggccatggt tattcccaac
     6001 agctttattt tcgatcaccc cttaatcaat gaaacctata caaaaaaata ccgcctccat
     6061 attgtgaccg tcccgttaag taccgatgat gattggaaaa ctgccgaacg cctcctgctg
     6121 caggccggtg aagaagaggc cgcccccttt atcgatgagg caaggacgta tctcaaaaaa
     6181 ctcgaaggca agttgtggtt ggacgccccc tccgttgaac cacgggtcac cattcagttg
     6241 cctgagccag gccgtattaa tttattactc cgcgtgccct gcccgactca atatccctca
     6301 cgcctggaac aagcgatcct tcgaagattt cttgctgaat tttcctttgc tccccgcctg
     6361 gttatacagg aatctcattc ttctcattag cagtggatta gcctttccac cggtttttat
     6421 ggtaaaccct tgtaactcgt tttcataggt cagcatcctc gctgaccagt cattaaccca
     6481 aacattgccg attccacgat ccacatcgag ggcctactca acgggtaggg ccgaggacaa
     6541 gaactgaata tcccgattca ggaagggcac atccatgatt gtcgacatac tcgagaatga
     6601 tccactctgg tacaaagacg ctattattta tgaactccat gttcgggcgt tttgcgatag
     6661 taacgccgat ggcataggcg atttcaaagg gcttacccaa aaactcgatt atctccagga
     6721 tctgggtgtg accgcgatct ggttgctgcc cttttgttca tctcctctcc gggacgatgg
     6781 ctatgatatt tccgactaca ccaacattca tccacattat ggaaccaggc gagactttca
     6841 agcgtttgtc cgggaggccc atcgtcgcgg attacgagtg attacggaat tagtggtgaa
     6901 tcacacctcg gatcaacatc cctggtttca gcgcgcgcgg aaagcccccc ccggcagcaa
     6961 atggcggaat tggtacgtgt ggagtgagac cccggatctc tattccgaca cccgcattat
     7021 ttttaaagat accgaacatt cgaattggac ctgggacccc gtggccaagg cgtatttctg
     7081 gcatcgtttt ttttctcatc agccggacct gaattacgac aaccctgagg tgagaaaggc
     7141 gatctttgag gtcctggatt tctggctgga actgggcgtg gacggcttgc gcctggatgc
     7201 cgtgccctat ctcttcgagc gagaagggac caattgcgaa aaccttccgg aaacgcatgc
     7261 gtttctcaaa acgctgagaa cgcacgtcga taaaaaattc aaaaaccgtt tctttcttgc
     7321 ggaagccaat caatggccgg aagatgccgc ggcctatttt ggggagggcg atgaatgtca
     7381 catgaacttt cattttccct tgatgccccg cctgttcatg tcaatgcaaa tggaagatcg
     7441 gtttccgatt atcgatattt tagatcagac tcccgcgatc ccggaagcct gccaatgggg
     7501 acttttttta cgcaatcacg atgaacttac tcttgaaatg gtgacggatg aagaacggga
     7561 ttacatgtac cgcgtctttg cccatgataa gcaggccagg atcaatctgg gaatccggcg
     7621 gcgattgact ccgctccttg ggaatgaccg gaaaaagatt gaactgatgt acagcctgtt
     7681 attctcgatg ccgggcacac cggtcatcta ttacggggaa gagatcggga tgggggataa
     7741 tttttattta ggcgaccgga acggggtcag gactcccatg caatggagtg cggatcgcaa
     7801 tgccggattt tcctatggca atccgcagaa actctatctg cccattatca ttgaccctga
     7861 ataccattac gaagccgtca acgtcgaact ccaacaaaat aacgcgcagt cccccctctg
     7921 gtggatgaag cgcatcgttt ccttgcgtaa acgctataag gtctttggac gaggctccat
     7981 tgaatttctt catccgtcca atcggaaagt cctggtcttc ctccggcgct atcaggacga
     8041 aaccattctg gtggccgtga acttatcccg ccatgcccaa tgggtcgaac ttgatctcgc
     8101 tgaattcaaa ggacgacgtc ccatgacctt gttcggacgg agtaaatttc cagccattgg
     8161 cgatttgcct tatttgctca cgttgagcgg acacgccttt tattggtttg ccttggagcc
     8221 ggttgaatcc aagcaactcg aggctcaggg aaagacggaa cacggcttac ccaccatcac
     8281 gatctccaaa gactgggata acctcatcca gaaacgggaa aaggtcaaac tcgaaaatgt
     8341 gttaccccag tacttacaag gccgacggtg gtttggcggc aaggcccgca ccatgcaatt
     8401 tgtggaagtc acggaaacga tccctcttcc gcaggaaaat cctttggcag tcctcgcgct
     8461 catccgagtg gaatata
//
LOCUS       DATIAL010000363         4211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_282816, whole genome shotgun sequence.
ACCESSION   DATIAL010000363 DATIAL010000000
VERSION     DATIAL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282816"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VLA60_11180"
     CDS             complement(<1..568)
                     /locus_tag="VLA60_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HSF09967.1"
                     /translation="MKPAFKRELTTVLCTILVGLSLTSTGIGSETSLDSAVGMITVDG
                     HAVPDIGPLPTVVPQPPGNLNYAAKISLGKQLYFDGRLSKNNEISCAFCHNPVVGFAD
                     PNQTSAGVGGLRGGRQSPTVYNTAFNPFQFWDGRAGSLEEQAIGPIHNPVERAETHEH
                     VVPKIAKINGYKEEFQEVFGTGVSLQGIA"
     gene            790..3312
                     /gene="hrpB"
                     /locus_tag="VLA60_11185"
     CDS             790..3312
                     /gene="hrpB"
                     /locus_tag="VLA60_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HSF09968.1"
                     /translation="MKTHTYPIDAVFPQLQQSLRQHLIVLLTAQPGAGKTTQIPLALL
                     QEPWLIPKTIIMLEPRRLAARAAARRMSDLLGETVGTTVGYRTRLDTKISPNTKLEVV
                     TEGILTRMLQHDPSLQNYGLVIFDEFHERSLQADLGLALCLESQKVFREDLRLLIMSA
                     TLDSAAISQQLRQAPVISCEGRMFPVETRYVGKPDGKNFAMQVAQSIHRLLKTEQGNL
                     LVFLPGAGEIRQVERLLADLPLGPHTRIAPLYGDLSAQAQDQAILPPPAGWRKVVLST
                     NIAESSLTIEGIRLVIDTGLMRVPRFDSRSGMSRLATLTVSQQSAEQRRGRAGRLEPG
                     LCIRCWSEAEQRTFTPRTTPEILDADLTSLVLELSLWGNHDPQELLWLDPPPSGAIAQ
                     ARQLLHSLGACDTHGHITDHGRAMADLPMHPRLAHMVLNGKALGMGTLACDLAAALSE
                     RNLFKGSFAREHADLRTRFDMLYGGAPVQRNTGSLDMTTIQRIRQASQSWQRTLHITT
                     PHHVPKQQIDQLGVLLALAYPDRIAQRQSDEDRRYRLANGRSARFHHPDPLEHEEWLV
                     IADLNGAPATALIYMAAPISREDLLSHCGDLIQSTDSVMWDGSTQAVRSIRQRRLGEL
                     ILEESRLPDPDPDLVLTALLDGLRNTGLSCLPWNPTLRNWQARVQFLRRATELGSAWP
                     DVSDDTLLQTLDQWLGPFLSNLSSLNQLKRIDLAWPLQALLSPEQRRTLDTLAPTHLT
                     VPTGSHIPLDYLSGEIPILAVRLQELFGQCDTPRLANGRVPVLIHLLSPARRPVQVTQ
                     DLTSFWKTGYTQVKKELKGRYPKHFWPDDPLQAPPTRGIKKQ"
     gene            complement(3566..4159)
                     /locus_tag="VLA60_11190"
     CDS             complement(3566..4159)
                     /locus_tag="VLA60_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09969.1"
                     /translation="MGKEQIIRIPDEDRVREVSPKIRAIDEHTFQDPQESKSLWKKTK
                     SFLLRHSLLTNISNALFKKMLLATGIRSPDKPKKRSIYRLFEDSEVLTQFSLSPFAEK
                     TKKAIAVWGEDAKLQGYHLIFLLIPPRSDFNNADIYNQVKEFLRGQKIDFIDFAPIFK
                     ERGWSREDLYWQIDQHLNDEGNRRVGQELSRLNNGKM"
BASE COUNT         1026 a         1192 c          972 g         1021 t
ORIGIN      
        1 cggcgattcc ctgcaaactc accccggttc cgaatacttc ctggaactct tccttatagc
       61 cgtttatctt cgcaattttg ggaacgacat gttcatgggt ctcggccctt tcaacgggat
      121 tatgaatagg accaatggcc tgttcttcca acgagccggc tcgaccatcc caaaattgaa
      181 aggggttaaa ggcggtattg tacactgtag gcgattgccg cccgccccgc agccctccaa
      241 cacccgccga ggtttggttg ggatcggcaa atcccacaac agggttatga caaaacgcac
      301 aagagatctc gttgttcttg gataaccgtc catcaaaata cagctgtttt ccaagagaaa
      361 tttttgccgc gtaattgagg ttgccgggag gctgtggaac aacagtcggc agtggaccaa
      421 tgtcgggaac cgcatgacca tcaaccgtga tcattcccac tgctgaatca aggctcgttt
      481 cactccctat tccagtggaa gtaaggctca gtcctaccag gattgtgcaa agtactgtgg
      541 tcagttccct tttgaatgct ggcttcattc cgtctttccc tcctgatcag tttgatgccg
      601 tttccgatgg acaaaacccc ttattcaata cttcagtgta ccctgaatgt cagcaaaata
      661 aaaagacaat tgcttcatct atagtaaaga tagatgctcc aacttggttt tttcaggtaa
      721 gttacttttt aacatggggg ccttgctgag aacctctgat tcgctattcc acctcacaat
      781 gaatgttgca tgaagacaca cacgtatccc attgatgcgg tatttccaca actacagcaa
      841 agcctcaggc aacacctaat cgtccttctc accgctcagc cgggtgccgg gaaaacgaca
      901 cagatcccgc tggcccttct ccaagaaccg tggttgattc caaaaaccat catcatgctg
      961 gagcctcgac gattggccgc gcgtgcggcc gcgcgccgta tgtccgatct tctgggggaa
     1021 accgttggca ctaccgttgg atatcgcaca cggttggata ccaaaatcag cccgaacaca
     1081 aaactggaag tggtaaccga aggcattctc acaagaatgc ttcaacacga tccatcatta
     1141 cagaactatg gcctggtcat ctttgatgaa ttccatgaac gcagtctgca agccgatctg
     1201 ggtctggcct tatgcctgga atcccagaag gtatttcgag aggaccttcg gctgttgatc
     1261 atgtcggcca cactcgacag tgcggccatc tcgcaacaat tgagacaggc tccagtgatc
     1321 agttgcgagg gcaggatgtt tcccgtcgaa acccgctacg tcggaaagcc ggatgggaaa
     1381 aatttcgcca tgcaggtggc ccaaagcatt catcgtctgc tgaagaccga acagggaaac
     1441 ctcctggtgt ttcttccggg tgcgggagaa atccgccagg tggaacgact cctggcagac
     1501 ctgcctcttg gcccacatac gcgcatcgct ccgctctatg gggatctatc cgctcaggcc
     1561 caggaccagg ctattcttcc accgcctgcc ggatggcgaa aagtggtcct atccactaat
     1621 attgccgaat ccagtctcac catcgaaggc attcgtctcg tcatcgatac cgggctcatg
     1681 cgcgtaccac ggtttgattc acgtagcggt atgagccggc tggccaccct cacggtctcc
     1741 caacaatcgg ccgaacagcg tcgcgggcgg gcgggacgcc tggaacccgg cctgtgtatt
     1801 cggtgctggt ctgaggccga gcagcgtacc ttcacccccc gaaccacacc ggaaattctt
     1861 gatgccgacc taacctcgct cgtgttggag ttgtctctat ggggaaatca tgatccacag
     1921 gaattactct ggcttgaccc tcctccttcc ggagccatcg ctcaggcacg acagctcctt
     1981 cactcccttg gcgcatgtga tactcatggc cacatcaccg atcatggccg ggcgatggcg
     2041 gatctcccca tgcatccgcg gctcgcgcat atggtcctga atggaaaggc gctgggaatg
     2101 ggaaccttag catgcgatct cgccgcagcc ttaagcgaac gaaacttgtt caaaggatca
     2161 ttcgcacgag agcatgccga tcttcgcacc cggtttgata tgctctatgg tggcgcccct
     2221 gttcagagaa acaccgggtc cctggatatg acaaccatcc aacgcattcg acaggcctct
     2281 cagtcatggc aacggacact gcacatcacc actcctcacc atgtccccaa acaacagatt
     2341 gatcagttgg gtgtactcct ggcgctggcc taccccgatc ggattgcgca acgacaatca
     2401 gatgaggaca gacgataccg cctggccaac gggcgaagcg caagatttca tcacccggat
     2461 cccttggaac atgaagaatg gctcgtgatc gcagatctta acggtgcccc ggcaacggca
     2521 cttatctata tggctgcccc gatttcacgt gaagatctgc tctcccactg cggggatctc
     2581 atacagtcaa cggattctgt gatgtgggat ggctcaacgc aagcggtcag atccatccgg
     2641 caacgacgac ttggggaact cattctggag gaaagccgcc ttcccgatcc tgatcctgat
     2701 ttggtgttga cggcgctcct cgacggcctt cgaaacacag gcctctcctg tttgccctgg
     2761 aaccccacac tcaggaactg gcaggcgcgc gtccaatttc tgcgccgcgc cacagaactg
     2821 ggatccgctt ggcccgatgt ctcggacgac acactccttc agacattgga ccagtggctt
     2881 gggccttttc tcagcaatct ctccagcctc aatcagctga aacggatcga tcttgcctgg
     2941 ccgcttcagg cgcttctttc tcctgaacaa cggcgcacgc tcgacaccct ggcgcccacc
     3001 catctcacgg tgccgacagg atcgcacatt cccttggatt atctgtcagg agagattccc
     3061 attctggctg tccgtcttca ggaattattc ggacaatgcg acacgccccg cctggccaac
     3121 ggaagagtcc ctgtcctcat tcatcttctc tctccggcca gacgccccgt tcaggtcacc
     3181 caggacctca ccagtttttg gaaaaccggt tacacgcaag tgaaaaaaga attgaagggc
     3241 cgctatccca aacacttctg gcccgatgat ccacttcaag ccccgcctac tcgagggatt
     3301 aaaaagcaat aattcaaagt ggcctgtgac ttaagccgta tggaccctca tctacctcga
     3361 tttagaaggc gagcatggat tcatccaaac caatccagtc atgtcactcg ggttggcatc
     3421 aacaaactgt gttccggcaa aaaaggggtg gctccccaag aatggaaaat attcagtcat
     3481 tacctccggg aagggttatg cttactcatc cagatgccaa aaaattttcc agagatgtta
     3541 ccatgacact gttaccgata aacaactaca tctttccatt attcagccgt gagagttcct
     3601 ggcccactct gcgatttccc tcgtcattca aatgttggtc aatttgccaa tagagatctt
     3661 ctcgactcca acccctttct ttaaagatgg gagcgaagtc aataaagtcg atcttttggc
     3721 cacgaagaaa ttctttcacc tgattataga tatcggcatt attgaaatca cttcttggtg
     3781 gaattaataa aaaaattaaa tgatatcctt gcaatttcgc atcttcgccc caaactgcaa
     3841 tcgctttttt ggttttctcc gcaaaaggag aaagagaaaa ttgggtcaaa acttctgaat
     3901 cctcaaacaa cctataaata cttctttttt ttggtttatc gggagaacga attcctgttg
     3961 ccagaagcat ttttttgaaa agggcatttg aaatattcgt caacaatgaa tgccgtaaca
     4021 aaaaactctt tgttttcttc cataaggatt ttgattcttg gggatcttga aacgtatgtt
     4081 cgtcaattgc ccttattttc ggggaaacct cacgaacccg atcttcatcg ggtatacgaa
     4141 tgatctgctc ctttcccatt tcataaaccg tatcgatgag ccaaccatcg acgactattg
     4201 aatgtggaaa g
//
LOCUS       DATIAL010000364         7336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1141104, whole genome shotgun sequence.
ACCESSION   DATIAL010000364 DATIAL010000000
VERSION     DATIAL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141104"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VLA60_11195"
     CDS             <1..695
                     /locus_tag="VLA60_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF09970.1"
                     /translation="LRTFGWLFRPPVRITQIIKQMHFVGFKSSFVVILTALFTGMVLA
                     LQGYYSLRKFGSEGLLGSAVAISMIRELGPVLASLMVTARAGSAMTAEIGIMRITEQI
                     DALETMAINSLQYLVTPKVVASLISVPLLVAMFDVVGIWGGYLVGVKLLGVSGSSYWS
                     SIESAVEWKDVYGGILKSISFGLIISWICCYKGYYTRMSSEGLGKATTESVVLAAVLI
                     LVWDYFLTSVLM"
     gene            704..1471
                     /locus_tag="VLA60_11200"
     CDS             704..1471
                     /locus_tag="VLA60_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HSF09971.1"
                     /translation="MSTSLPIIVKLVEVEKAFGDQSVLKGVTLDIPKGKITTIIGPSG
                     EGKSVMLKHIIGLIRPDRGKIIVDGIDLTRINDRELNDIRKKFAMLFQSAALFDSMNV
                     FDNVAFPLREKRRAAEQEICRLVQEMLDRVELGDMGHKFPAELSGGMKKRAGLARALV
                     MRPEIILFDEPTTGLDPLMAHAIHKLILAMQKTFGFTGIMVSHQIPEIFPISDWVAML
                     RNGKIIAMSPSDEFQQTADPVVREFISVNNGIHLASV"
     gene            1591..2037
                     /gene="mlaD"
                     /locus_tag="VLA60_11205"
     CDS             1591..2037
                     /gene="mlaD"
                     /locus_tag="VLA60_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HSF09972.1"
                     /translation="MMERGKLELIVGIFVLVGVICLGYLAIKLGKLELVGGDYYELQA
                     EFSSTSGLKNGASVEIAGVEVGRVKKIGLKEDRAQVVLAIQDGIPVFDDAIASIKTRG
                     IIGEKFMELSPGGAGERLKTGGTIVDTESGIDLEQVISQFIHGNVE"
     gene            2070..2762
                     /locus_tag="VLA60_11210"
     CDS             2070..2762
                     /locus_tag="VLA60_11210"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF09973.1"
                     /translation="MHREWVKKPELKAGTRAGELQRLQFKNSSRKVAVLFLGWIVVSL
                     ACLAVAWGGGTPTGAVKETVDQVFVVLRDQALKDPARETERRAKLEEIIGKRFDYAEM
                     AKRTLASQWKGLSPEQQQEFVTLFQQFLANSYVGNVDGYSGEEVEYLKEREKGEFAEV
                     QTKVVSPKVQIPLDYRLLQKNGEWWVYDVVIDGVSLMKNYRGQFSRIINSSSFEALLE
                     KLRSKADLGTSS"
     gene            2775..3983
                     /locus_tag="VLA60_11215"
     CDS             2775..3983
                     /locus_tag="VLA60_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSF09974.1"
                     /translation="MISFRLWGLVLSCLALGGVFPPSSIVLAENSFFVVPAFSTSKND
                     GQDFGLIAPSLNSDAEGNLRSLFAPMLIHNSFLGVRGTLNYFHYWSGGRQMETVGSYT
                     EEIERKLKFRYQDPGFIEGLFFVDVGAQFFKNATKRFYGLGQTTPKGNESNYTGREIQ
                     IHWNFGIHLNDVTRLSVNQRFRNVEIQPGGVDDEPYTKDRFPRDPGIKGATILAHRLV
                     FQYDSRDNLNTPTAGTRVEAFGELAQNFDFGKEEDLLYFRSGFDIRHLIPSPSKRYIF
                     VARAMLQLSFGDGIPFYEQSSLGGEDSLRGYGRDRFIDKHMVAFNVEERIHLFGMKMF
                     NVSIECELTPFVDMGRTYKDFKFRQFHDWEVTPGVGFRAIVRPNVMARVDWGYSREGG
                     AVFAGLNYPF"
     gene            4078..4770
                     /locus_tag="VLA60_11220"
     CDS             4078..4770
                     /locus_tag="VLA60_11220"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HSF09975.1"
                     /translation="MVGVFILPSGALQGEDSTDQQYPESLERELEILVKEGAANSNDL
                     TRLLRMAGLYLDLGYGVYVDREQKLAAFQEGARLAQKAFDLRESSVDAHFLFAANLGS
                     AAELEGLVAAALTIQKLKKHVSRVLELDPKNVQAHHMLGRMYEELPWMLGGDQNLAGE
                     HLKKAVSLDSRYAPAGLDLAKWYLKHGQNLEAVRELTRVVSTPPLRKRWIWERIHRPE
                     AQALLQQTLSQG"
     gene            4916..6811
                     /gene="dxs"
                     /locus_tag="VLA60_11225"
     CDS             4916..6811
                     /gene="dxs"
                     /locus_tag="VLA60_11225"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HSF09976.1"
                     /translation="MSLLKRIESPADLKKLSRDQFPELCQEIRELIIEVISHVGGHLA
                     SNLGVVELTVALQYLLNTPDDKIVWDTSNQAYTHKLLTGRREQFHTVRQFGGISGFCK
                     REESIYDTFNAGHAGTGVSAAVGFVEAREQLGKSNKVVCVVGDGALTAGMTLEGLQHA
                     GDMGRDCVVILNDNQMSISRNVGAISAYLNRTFTGEFYTRLREETSQLLETIPQIGEP
                     VKRMAIRAEELAKGLLLPGLLFEELGFRYVGPIDGHNFEHLLPTLENVLKLKGPTLLH
                     VITKKGLGFQPAMKNPVWFHACSPFDSKTGQPMKKPGHPSYSSIAAKTLIRQAKKDNR
                     VVVITAAMCEGTGMSEFEKEFPSRLIDVGIAEQHAVTYAAGLAADNMRPVICMYSTFL
                     QRAYDQVVHDVATQNLPVTFCIDRGGLVAEDGTTHHGAFDFAFLRHVPNMVIMAPKDE
                     NELQHMVQTGLVHDGPVAVRYPRGSSLGVPLDSEPIPLSIGQGELLSDGRDVAIIAIG
                     VMVSEAMKAAERLQEEGVSVAVINARFVKPLDKNLIREIAKKVKCLITVEEGCRMGGF
                     GSAVLEFLSEEECWSMPTKVLGLPDWYIEQGPQDLLREKYGLTADGIYDQAKALHDRV
                     SLQAVIR"
     gene            6821..>7336
                     /locus_tag="VLA60_11230"
     CDS             6821..>7336
                     /locus_tag="VLA60_11230"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSF09977.1"
                     /translation="MYISRRKTKQIQVGSVKVGGDAPISVQSMTIPHPRDVAGTVEQI
                     HRLEKAGCELIRVAVPDMEAAEALPKIKSQMTVPLIADIHFDHRLALKAAKVVDCVRI
                     NPGNIGPWWKTEEVIQAVNDYGIPLRIGVNGGSLEKHLLEKYGYPTAEALAESALNAV
                     HALEDVGFTNMK"
BASE COUNT         1852 a         1426 c         2055 g         2003 t
ORIGIN      
        1 tgttacggac gtttggatgg ttgttccgac caccggtgcg aatcactcaa attatcaaac
       61 aaatgcattt tgtgggtttc aagtcatctt ttgttgtgat tctgacggcg ttatttaccg
      121 ggatggtgtt agctcttcaa ggatactatt ccctgaggaa gtttgggtct gaaggtttgc
      181 tagggtctgc tgtggcgata agcatgatcc gtgagctagg tccggtatta gcctcattga
      241 tggtaacggc tcgcgcggga tcagccatga ccgcagaaat cgggattatg cgtattaccg
      301 agcaaattga cgcattggag accatggcga tcaattcttt gcagtatttg gttaccccca
      361 aggttgttgc ctcgcttatt tcggtaccct tgttggttgc aatgtttgat gtggtgggga
      421 tttggggagg gtatctggtt ggagtcaagt tgctcggagt cagcgggagt tcatactgga
      481 gttcaatcga gtcagcggtg gaatggaaag atgtctatgg gggaatatta aagtcgatca
      541 gctttggcct gattatcagc tggatctgct gttataaagg gtattatacg agaatgagtt
      601 ccgagggatt gggaaaagcc accactgaat ctgtggtgtt agccgcagtt ctcattttgg
      661 tttgggacta ttttttaaca tccgtcctta tgtaacgtta cgcatgtcta cgagtctacc
      721 gattatcgtc aagctggttg aggtggaaaa agcattcggg gaccagtctg tgcttaaagg
      781 ggtcactctg gatatcccta agggtaagat caccacgatt atcggtccga gtggtgaggg
      841 gaaaagtgtg atgctcaagc acatcattgg attgattcgc cctgatcgag gcaagatcat
      901 cgttgatgga atagacctta cacgaattaa tgatagggaa ttgaatgaca tacgcaagaa
      961 atttgctatg ttgtttcaat cggccgcctt gttcgattcc atgaatgtat ttgataacgt
     1021 cgcgtttccc ctgcgggaaa agcgcagggc tgctgaacag gaaatatgtc gattggttca
     1081 agaaatgctg gacagggtgg agttgggaga catgggtcat aaatttccag cagaactgag
     1141 cggagggatg aagaagcgtg caggtttggc gagagccctg gtgatgaggc cggagattat
     1201 tttatttgat gagccgacga cagggctgga tccgttgatg gcccatgcca tccataagct
     1261 cattttagct atgcaaaaaa catttgggtt tacgggtatc atggtgagtc accaaattcc
     1321 agagatcttc cctatttctg attgggttgc tatgttaagg aacgggaaga ttattgctat
     1381 gagcccttca gatgaatttc aacagactgc cgatcctgtg gttcgtgagt tcatttcggt
     1441 caataatggc attcatctcg cttctgtctg aatcgtggct cttgatggtg gtttaaggag
     1501 tcggatcaat atttagtcac gccgtactat gagatttatc cttcctggtc cttgccccaa
     1561 taaaacatca attaaccgaa ctgaattatt atgatggaac gtggaaaact tgaattaatc
     1621 gtcggaatat ttgtgttagt gggggtcatc tgtcttgggt atttggcgat caaacttgga
     1681 aagcttgagc tggttggagg ggattattac gaactccagg cagaattttc ttccacctcc
     1741 gggttgaaaa acggagcgtc ggtagaaatc gccggagtag aggtgggccg agtaaaaaaa
     1801 atcgggctga aagaagatcg ggctcaagtg gtccttgcga tccaggatgg gatccccgtg
     1861 tttgacgatg caattgcttc aatcaagact cgtggcatta tcggagaaaa attcatggaa
     1921 ttatcacccg gcggggcggg agaacgtttg aagactggcg ggaccattgt ggacactgaa
     1981 tctggaattg acctagagca ggtgattagt caatttatcc atggcaatgt tgagtgaggc
     2041 ttgtttggtg aaatggatgt ggaggaaata tgcataggga atgggtaaag aaacctgaat
     2101 taaaggcggg gacgagagca ggtgaacttc aacggttgca gtttaaaaat tcctcaagaa
     2161 aagtggcagt actatttctt ggttggatag tagtttctct tgcctgtttg gctgtcgcgt
     2221 ggggaggcgg tactccaacg ggggcagtca aggaaaccgt tgatcaggtt tttgtggtgc
     2281 tgagggatca agcgttgaag gaccctgcga gagagacgga acggcgagcg aagctggaag
     2341 aaattatcgg aaagcgattt gattatgccg aaatggccaa gcgtaccctg gcttctcagt
     2401 ggaaggggtt aagtccagag caacaacaag aatttgtgac tttatttcag cagtttttgg
     2461 caaattccta cgtcggcaat gtagacgggt attccgggga agaagtggag tacctcaaag
     2521 agcgggaaaa gggagaattt gctgaagttc agaccaaagt ggtgtccccg aaagtgcaaa
     2581 tacccttgga ctatcgtctg ttgcaaaaaa atggagagtg gtgggtgtat gacgtagtga
     2641 ttgatggagt gagtttaatg aaaaattacc gtgggcaatt ttcccgtatt attaactcct
     2701 catcgtttga agctcttctc gagaagcttc gttcaaaagc cgacctgggg acttcgtcct
     2761 aaggagtcgg ttttgtgata tcgtttcgtt tgtggggcct ggtgttgtcg tgcctggcct
     2821 taggcggcgt tttccctccc tcttccatcg tcctcgccga aaattctttt tttgtggttc
     2881 ctgcgttttc tacttccaaa aatgatgggc aagactttgg gttgatcgcc ccgtcattga
     2941 attccgatgc ggaaggtaac cttcgttctc tctttgcccc gatgctgatt cacaattcat
     3001 ttttaggagt ccggggaacc ttgaactatt ttcattattg gtctggggga aggcaaatgg
     3061 agacagtcgg ttcgtatact gaggaaattg aacgaaagct gaagtttcgt taccaggatc
     3121 ctgggtttat cgaaggtttg ttctttgtgg atgttggagc acagtttttc aaaaatgcga
     3181 ccaagcggtt ttatggctta gggcaaacca ctcccaaggg gaatgaatcc aactatacgg
     3241 ggcgtgaaat tcaaatccac tggaactttg gtatccattt gaatgatgtg acgcgattat
     3301 ccgtcaacca aaggtttcga aatgttgaaa ttcaaccggg aggtgtggat gacgagccat
     3361 atactaagga tcgtttcccg cgggatcctg ggataaaagg tgcgacgatt ttggcccacc
     3421 gtctggtgtt ccaatatgat tccagagata acctcaacac cccgacagcg ggcactcgag
     3481 ttgaagcatt tggggaattg gcgcagaact ttgattttgg gaaagaagag gatctgttgt
     3541 attttcgttc agggtttgat atcagacacc tgattccgag tccctccaaa cggtatatat
     3601 ttgtggctcg agcgatgctg caattaagtt ttggcgatgg gatccccttt tacgaacaaa
     3661 gttccctggg aggagaagac agcctgagag gatatgggag ggatcggttt attgataaac
     3721 atatggtggc atttaatgtt gaggaacgta tacatctatt tggcatgaag atgttcaatg
     3781 tcagtataga atgtgaatta acgccatttg tggatatggg gagaacctat aaggatttta
     3841 aatttcgtca atttcatgat tgggaagtta cccctggagt aggatttcga gccattgtcc
     3901 gccccaatgt catggctcgg gttgattggg ggtatagtag ggaaggtggg gcggtatttg
     3961 ctggcctcaa ctatccattc tgatacagct cacagtcgtt ctgctcttcc ggaaaaacat
     4021 gtattgacaa gacccaggaa tatgcgtcta tttgtgccaa tagggtttct cttcctaatg
     4081 gtaggagtct tcattctccc ttctggggca ttgcagggtg aggactcaac cgaccagcaa
     4141 tatcccgaga gtcttgaaag ggagctggag attttagtga aggagggggc cgctaattca
     4201 aacgacctca cacgcttgct tcggatggcc ggattgtatt tggatttggg ttacggggtg
     4261 tacgttgatc gggagcagaa gttggcagct ttccaggagg gagcccgtct cgcccaaaaa
     4321 gcctttgatc ttcgtgagtc ttctgtagat gcccattttt tgtttgccgc caatctcggg
     4381 agtgctgcgg aattggaggg tttggttgca gcggcattga cgattcagaa gttaaagaag
     4441 catgtgagtc gggtgttgga acttgatcca aaaaatgttc aggctcacca tatgttaggg
     4501 cggatgtatg aggagttacc ctggatgtta ggaggcgatc agaacttagc tggagagcat
     4561 ttgaaaaaag cggtttctct cgatagcagg tatgcaccgg cagggttgga tcttgcgaaa
     4621 tggtacctga aacacgggca aaatttggag gcagtgaggg agctaacgcg ggttgtaagt
     4681 accccacctc ttagaaagcg atggatatgg gaacggattc atcgaccgga agcccaggcc
     4741 ttgctccagc agactctcag ccagggttga ccgatctact gctcatccct gaagttagat
     4801 caaatgcttg acaggcaata gcagctatgg gaagattttg gttaatcatg tcggaatttg
     4861 gacattttta gttattcccc tacaacacgt taggttcttc aagaaaggat gcgcaatgtc
     4921 cttgctaaaa aggattgaaa gtccagcaga tttaaagaaa ctttctcggg accaattccc
     4981 tgaactttgt caggaaattc gggaactcat tatagaagtg atctcccatg tgggcgggca
     5041 tttagcctcc aatttaggtg tggtggagtt gacggtggct ttgcagtatt tgttgaacac
     5101 gccggacgat aaaattgtat gggataccag taatcaggct tacacacaca aactcttaac
     5161 gggccgtcgt gaacaattcc atacggtccg ccagtttggg ggaataagcg gattttgtaa
     5221 acgagaagaa agcatctatg acacgtttaa tgccgggcat gccggaaccg gagtgtccgc
     5281 cgccgtgggc tttgtagagg cacgtgagca attaggcaag tccaataagg tagtttgcgt
     5341 tgtaggagac ggggccctca cggctggaat gacattggaa ggattgcagc atgccggaga
     5401 tatgggccgg gactgtgtgg tgatcctgaa tgataatcaa atgtcgattt cccgaaatgt
     5461 cggagctatt tccgcctatc ttaatcggac ctttaccggg gagttctaca cgagacttcg
     5521 tgaggaaacc tctcaactcc tggaaaccat tccgcaaatt ggagaacccg taaagcgaat
     5581 ggcgattcgg gctgaagagc tggccaaagg cttattgctc cctgggctgc tatttgaaga
     5641 gttaggattt cgatatgtcg gtccaattga tgggcacaat tttgagcatt tacttccgac
     5701 gttggagaat gttttgaaat taaaagggcc aactctcctg catgtgatta ccaaaaaagg
     5761 attgggcttt cagccggcaa tgaagaatcc ggtttggttc catgcctgtt ccccttttga
     5821 ttcgaagact gggcaaccga tgaaaaagcc cggtcacccc tcatatagtt ctatcgccgc
     5881 taaaacactg attcgtcaag caaaaaaaga taaccgtgtg gtcgtcatta cggctgcgat
     5941 gtgtgaggga acgggaatgt ctgagtttga aaaggagttt ccctcccgtt tgattgatgt
     6001 cggaattgcc gagcagcatg cagtgacata tgcagctggt ctcgccgcgg ataatatgcg
     6061 gccggttatc tgtatgtact cgacctttct gcagcgggcc tatgatcagg ttgtgcatga
     6121 tgtggcaacc caaaatctgc cggtgacctt ttgtattgat cggggaggac tggtggccga
     6181 agatggaacg acccatcatg gggcttttga ctttgcgttt ctgcggcatg ttcccaatat
     6241 ggtcattatg gccccaaaag atgaaaatga acttcaacat atggtccaaa caggattggt
     6301 gcatgatggt ccggtggcgg ttcgatatcc tcgcggcagt agccttgggg tgcctttaga
     6361 ttctgagcca attcctcttt caatcggtca gggtgagctg ttatctgatg ggcgagatgt
     6421 ggcgattatc gcgattgggg ttatggtttc ggaggcgatg aaagcggctg agcgcttaca
     6481 agaagaaggc gtatcggtgg ctgtcattaa tgcacggttt gtgaaacctc ttgataaaaa
     6541 cctaattcga gaaattgcca aaaaagtgaa atgcttaatt acggttgaag aagggtgccg
     6601 aatgggtggg tttggatccg ctgttctaga gtttttatcc gaagaagaat gttggagtat
     6661 gccgacaaaa gtactcggat tgccagattg gtacattgaa caagggccgc aggatttgct
     6721 cagggaaaag tatggtctca cggcggatgg gatttatgat caggccaagg cgttgcacga
     6781 ccgggtttcc ctgcaagcag taatacgcta gtctattctc gtgtatattt ctcgacgaaa
     6841 aaccaagcaa atccaggttg gatcggtcaa agtcggtggg gatgccccca tatcggtcca
     6901 gtctatgacg attcctcatc cgagggacgt tgctgggact gtggagcaga ttcaccgctt
     6961 agagaaagct ggatgtgaat taattagggt agccgtgccc gatatggaag cggctgaagc
     7021 gcttcctaaa ataaaatcac aaatgacggt acccttgatc gcagatatcc attttgacca
     7081 tcgccttgct ctaaaagccg ctaaagttgt ggattgcgtc cgtattaatc cagggaatat
     7141 tgggccatgg tggaaaaccg aagaagtgat tcaggccgtc aatgactatg gcattccact
     7201 cagaattggc gtgaatgggg gttccttaga gaagcatctt cttgaaaaat acggctaccc
     7261 cacggccgaa gcacttgccg agtcggcatt gaacgcggtt catgctttgg aggatgttgg
     7321 gtttaccaat atgaag
//
LOCUS       DATIAL010000365         5658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1465192, whole genome shotgun sequence.
ACCESSION   DATIAL010000365 DATIAL010000000
VERSION     DATIAL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465192"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VLA60_11235"
     CDS             complement(<1..233)
                     /locus_tag="VLA60_11235"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HSF09978.1"
                     /translation="MRTERQGSKAARFMLNTCHSRGHAATLIDPLLYPLPLLDKMYKE
                     FKPGQAPQPLQQVADLIIPADGYIIVSGEYNHT"
     gene            585..1196
                     /locus_tag="VLA60_11240"
     CDS             585..1196
                     /locus_tag="VLA60_11240"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HSF09979.1"
                     /translation="MPLYPGMVQRPEDPPFQMEPVTDRKNGDKVHVSKISMSSHAGTH
                     IGAPLKCGSPYLDNEQTEFSATIGLAKVIEIQDRNSVKAEELKKHRIYSHDRILLKTR
                     NSASDWYSEHSPEDTIFLDRTAAVWLAERRVSTVGIDALLIGGKQENGKEIARILIQA
                     GIVIIEGLEFSRVKPGEYMLICLPLKIPNGNGAPARAILQPVR"
     gene            1347..2495
                     /locus_tag="VLA60_11245"
     CDS             1347..2495
                     /locus_tag="VLA60_11245"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF09980.1"
                     /translation="MPEIHHKEPSEFECEVSSPPPSSEFEAGHLSQLFQGPINIRSIA
                     LTGLFLYASLMVMYVAKAIMLPVFVALFLNLLFAPLVRGFEKFHIPPPLAAGGILLVL
                     ISTFTFGMVQLSTPASLWLDRAPEALQQVERKIRTIKSSVLEMGKATQALEHVASLSE
                     SRKAQKVEVKTNSLGGLLIDWTTEFILGLVSTMILLYFFLASGDLFLEKLVKVLPRFS
                     DKRRAVEIVRGIENNISSYLITVTSVNVGLALATSLAMYLLGMPNPFLWGAMAGILNF
                     IPYVGSIIGLGAVTLAAAITFEHMNMVGLVFGVYLFLTAMEGNFITPHLLGRKLTLNP
                     VIILVSVLFWGWLWGAVGALLAVPFVASLKIICDQIEPLNSIGEFLSR"
     gene            2583..3959
                     /locus_tag="VLA60_11250"
     CDS             2583..3959
                     /locus_tag="VLA60_11250"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HSF09981.1"
                     /translation="MPAWVLNWLNKLRSSLWLIPTCMTIAAILLSLAVPYLDAFFSPK
                     KIDSLRWLSNMGPEGAMTLLSTIAGSMITIAGVVFSITIVALTLASGQFGPRLLGNFL
                     RDRGNQIVLGTFVATFLYCLLVLRTIHVDPNGYTPYWGTLVGLVLAVCSLGVLIYFMH
                     HIAISIQAPNLIAAVYGELEEDLTRLFPERLGMPEGNETNETIIPSIMARIEKEGMDV
                     KAEVGGYLQAIENDELMEIARDQNLVILLHYRPGHFITKGASLAKVLALGPMADVVSQ
                     SINKQMICGNNRTQEQDIEFSVLQLVEVAVRALSPGINDPFTCINCIDHLSAILCQLC
                     QRKIPSPFRYDRDGVLRIIAPPVTFEGVLNAAFHQIRQHGKGNVAVLICLLEGLERIG
                     ETVERNEDRAAILHHVEMVQRASRESLQQEIDLEAVEDRCRQVMASLSMGVSRSSLLV
                     DKLLNILP"
     gene            4296..5069
                     /locus_tag="VLA60_11255"
     CDS             4296..5069
                     /locus_tag="VLA60_11255"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSF09982.1"
                     /translation="MLKTLLEMRGYGLRAIDKDLGSVNDFYFDDRLWRIRYLVADTGN
                     WLPGRSVLIGQEALERPDGENKVFPVKLTSAEIEDSPGIESDLPFSLQKEKELRLFFK
                     WREYWDEDGFIQPAGFTPMSVPGGEAIELSLKDGMKTSAKLNGNPHLRSANEIQGYYI
                     RARDGEIGHVDDFIVEDTTWAIRYLVVDTHNWLPGKKVLISYKWVNTVDWNQQTVEVA
                     MTRETIEGSPEYDPNKPINREYEVRMYDFYGKPHDWVEK"
     gene            5273..>5658
                     /locus_tag="VLA60_11260"
     CDS             5273..>5658
                     /locus_tag="VLA60_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09983.1"
                     /translation="MLNTFTNNPVPTVLIGLGLYWLLKDGAEPSHETQRPYRSDNTPW
                     RNSEKEFQPSMEEQVTEKFRTAKDALQGQVSEWKDEASHRVHELKEQASQQAKEWKVG
                     AGQKMEDVKGYLQEQGAVVRGEFNHL"
BASE COUNT         1498 a         1195 c         1409 g         1556 t
ORIGIN      
        1 atggtatgat tatactcgcc cgacacaata atgtagccgt cagccggaat aatcagatca
       61 gccacttgtt ggagaggttg aggcgcctga ccaggcttga attccttata cattttatcc
      121 aacagcggga gagggtagag aagaggatca ataagcgttg cggcatggcc gcgggaatgg
      181 caggtgttca gcataaaacg agcggcctta ctaccctgcc gctcggttct caccgattca
      241 taaaataggc caaattcatt ggcatgggat tttactttta gtcaaactaa atacatcgaa
      301 tgattcccgg atgtgtattt ccaaagtagg acctttattg tctgcacttt aaaccgtggg
      361 ttccgccaca gggtgtagac aaatctcaag tgggccttta caaaaggtct attgagccaa
      421 ggtatgaagt aaccatagtc tagcaatcgt gtcatccaat gccgggtttc ggaaaaggag
      481 gtatgataag agctggcctg ggctacctcc cggttttaaa tgagaagcga ttgagaagtc
      541 ggaatgtgga gtaggtcatg atatctaatt ggattgacat ttcaatgcct ctgtatccgg
      601 gaatggttca aagacccgaa gacccacctt ttcagatgga accggtgaca gacagaaaaa
      661 atggggataa ggttcatgtc tccaaaatat caatgagttc tcatgccgga acgcacattg
      721 gcgctccgtt gaaatgtgga agtccttatc tagataatga acaaacagag ttttcagcga
      781 caataggtct ggccaaagtg attgagatcc aagacaggaa ttctgtaaaa gcggaagaat
      841 taaaaaaaca ccggatctat tcgcatgatc ggattctttt gaagacacga aattctgcct
      901 ctgactggta ctctgaacac tcccctgaag atacgatctt tcttgatcga actgccgcgg
      961 tatggcttgc tgaacggcga gtgagcaccg tcgggattga tgcattgttg attggaggga
     1021 aacaggaaaa tggaaaagag attgctcgca tccttattca agccgggatt gtcattattg
     1081 agggactcga attttcccga gtgaaaccgg gtgagtatat gctcatctgt ttacccttaa
     1141 aaattccaaa cggaaatggt gctcctgcaa gggccattct ccaaccggtc cggtagtggt
     1201 gagggaaatt tttgctaggt agcccatgcc gatgaataaa tatccaatcc taaagaacga
     1261 caggatgagg aggtttgttg atagttcctg tgacacaaac ttaagttcgg tgcccccacc
     1321 tcttgtgccg aacttggctc cctccgatgc ctgaaatcca ccacaaggaa ccctcagaat
     1381 tcgaatgtga ggtttcctct ccacctcctt catccgaatt cgaagccggt catctatccc
     1441 aattatttca aggaccgatc aatatccggt ccattgccct cacgggactg ttcctttatg
     1501 cctcgctcat ggtgatgtat gtggccaaag ccatcatgct tcctgtgttt gtggccttat
     1561 tcctgaatct tttgtttgct cctcttgtgc ggggatttga aaaatttcat atcccccccc
     1621 cacttgcagc cgggggaatt cttctggttt tgattagtac ctttactttt ggaatggttc
     1681 agctgtctac tcccgcttct ctctggctgg accgggcccc ggaagcttta caacaggtag
     1741 aacgtaaaat ccggaccatc aaaagttcgg tcctggaaat ggggaaggcg acccaggctc
     1801 tggagcatgt ggcgagtctt tcggaatctc gtaaagcgca aaaagtcgaa gtgaaaacca
     1861 atagtttggg aggactattg atcgactgga cgacggaatt cattctgggg ctggtctcaa
     1921 cgatgatttt actctatttt ttccttgcct ccggggattt gtttttggaa aaactcgtga
     1981 aagtccttcc acgattttcg gataaacgac gggcagtgga aattgtccgt ggaatagaaa
     2041 ataacatctc cagttacctt attaccgtga ccagtgtgaa tgtcggcctt gcgctggcaa
     2101 ccagtcttgc catgtacctg ctcggcatgc cgaacccgtt tttatgggga gcaatggcag
     2161 gcattctgaa ttttattcct tatgtaggaa gcattattgg tttaggtgcg gtgacgttgg
     2221 ctgcggctat cacatttgaa catatgaaca tggtgggctt ggtgttcggg gtgtatttgt
     2281 ttttgaccgc tatggaagga aattttatta ccccccattt attgggacga aaactgacat
     2341 taaatcccgt cataatatta gtgagtgtgt tattttgggg ctggttatgg ggtgccgttg
     2401 gggcgttgct cgctgtccct tttgtcgcgt ctcttaaaat tatctgtgat caaattgaac
     2461 cactcaattc cattggggaa tttttaagtc gttagtttaa aatggttagg acacactctc
     2521 tgtctacttg gatttaggcg ggatgatgaa tttcaataat cttgctacaa ggtaagtaac
     2581 tcatgccggc ttgggtgtta aattggttga ataaactccg ttcgagtctc tggcttatac
     2641 ccacttgcat gaccattgcc gccatcctcc tctccttggc cgttccgtat ctcgacgcgt
     2701 tcttttctcc aaagaaaatt gactctcttc ggtggctgag caatatggga ccggagggag
     2761 cgatgacgct gctttccacc attgccggtt ccatgattac cattgccggt gtcgtgtttt
     2821 ccatcaccat tgtggccctg accctggctt caggacaatt cggtccgcgg ttacttggta
     2881 actttttgcg ggaccggggc aatcaaattg tgttgggaac atttgtggca acgtttctct
     2941 attgcctgct cgtcttgcga accattcatg tggatcccaa tggctatacg ccatattggg
     3001 gaaccttggt gggcctcgtt ttggccgtgt gtagcctagg ggtattgatt tatttcatgc
     3061 atcatattgc tatttccatt caggccccga atttaatcgc ggcggtatat ggggaattgg
     3121 aagaagacct gaccagactt tttccagagc gcttaggaat gccggagggg aatgagacga
     3181 acgaaaccat tattccctct attatggcca gaattgaaaa agaaggaatg gatgtaaagg
     3241 ctgaagttgg gggttacctt caggcaattg agaatgatga acttatggaa atcgcccgtg
     3301 accagaattt agtgatatta cttcattatc ggcctgggca ctttatcacc aagggagcat
     3361 cgttagccaa agtactggcc cttgggccga tggcagatgt cgtttcccaa tccatcaaca
     3421 agcaaatgat ctgtggaaac aaccgcaccc aggaacaaga tattgagttt tccgtacttc
     3481 aacttgtgga agtggccgtg agggcacttt caccaggaat aaacgatccg ttcacgtgca
     3541 taaactgtat tgatcacctc agtgcgattt tatgccagct gtgtcaacgg aaaattcctt
     3601 ctcccttccg gtacgaccgg gacggagttc tacggattat tgctccgcct gttacatttg
     3661 aaggcgtgct gaatgcggcg tttcatcaaa tccgacaaca cggaaaggga aatgtggcgg
     3721 tgctcatctg tctgttggaa ggcttagaac gtataggaga aaccgtggaa cgaaatgagg
     3781 atcgtgcggc aatcctacat catgtcgaaa tggtccaaag agccagccgg gaatccctcc
     3841 agcaagaaat agatttagag gccgtggagg atcggtgccg ccaggtcatg gcctcgttaa
     3901 gtatgggagt aagccggagt tcccttttgg tggacaaact gttgaatatc cttccctaac
     3961 atctctttca tttctgctcc tgggaccatt gatttggccc atttttccct ccggttccta
     4021 gttggtcgat tcagactctc aggttttcct gcatggtttt tctgtaaagt ttgaaagttg
     4081 ccaggatact ttctctaccc agaccattca atgactggaa acgctcgtac tgaggcaggt
     4141 gtttgacttt tcaggcaggc tgttaaaatt tttgtacatt gtcttagaag cctcagaaag
     4201 tggttaatga attcatagga gaaattccac ttattgctta tttgaaacag gtgactgatc
     4261 cattccttaa tacatattaa ttgaaaagga agattatgtt gaaaacttta ttggaaatgc
     4321 gagggtatgg attgcgagcc attgataaag atctcgggag cgtgaatgat ttttattttg
     4381 acgaccggtt gtggagaatc cgctacctgg tggcggatac cggaaattgg cttccgggaa
     4441 gaagtgtgct tatcggacaa gaagctctag agcggcctga tggagaaaat aaagtctttc
     4501 cggtgaagct gacctccgca gaaatcgaag atagcccggg catagaatcc gacttgccgt
     4561 tttccctcca aaaggaaaaa gaattacgtc tgtttttcaa atggagggaa tattgggacg
     4621 aggatggttt catccagcct gccgggttca caccgatgag tgtccctgga ggggaagcca
     4681 ttgaactttc cctcaaggac ggaatgaaaa cgtctgccaa attgaatggg aatccacatt
     4741 tgcggtctgc caatgaaatt caaggatatt acatcagggc ccgagacgga gagatcggcc
     4801 atgtggatga cttcatcgtt gaggatacca catgggccat tcgatatttg gttgtagata
     4861 ctcataactg gttgccagga aaaaaggttc taatttctta caagtgggtc aatactgttg
     4921 attggaatca acaaactgtt gaggttgcta tgactcgaga aacgatcgag ggaagtcctg
     4981 agtatgatcc gaacaaacct ataaatagag aatatgaagt tcgtatgtat gatttttatg
     5041 gtaaacccca cgattgggtt gaaaaatagt cgacaattac aaatctgttt taaaggtgca
     5101 attccccggt caatcaccgt catcgcctaa aaatttattg aatgccattt ggcaaccata
     5161 tattcgaaaa gaggaacgac cggagttcta ttgtgaggga acctttttat taccataccg
     5221 ggaaatacat tgcatatatc ggtctttgag catttactaa aggagagagt caatgttgaa
     5281 tacgtttacc aataatcctg ttcccaccgt acttatcgga ttgggattgt actggttgtt
     5341 aaaagatggg gccgaaccct ctcacgaaac ccaaagaccc tatcggagtg acaatactcc
     5401 ttggcggaac agtgaaaaag aatttcaacc ctcaatggag gaacaggtga cagaaaaatt
     5461 ccgaacagcg aaagatgccc ttcaagggca agtgtcggag tggaaggatg aggcgagtca
     5521 ccgggttcat gaattaaaag aacaggccag tcaacaggct aaggaatgga aagtgggggc
     5581 ggggcagaaa atggaagatg tcaagggata tcttcaagag caaggtgcag ttgtgcgagg
     5641 agaattcaat cacctatt
//
LOCUS       DATIAL010000366         6412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_819041, whole genome shotgun sequence.
ACCESSION   DATIAL010000366 DATIAL010000000
VERSION     DATIAL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819041"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..825
                     /locus_tag="VLA60_11265"
     CDS             301..825
                     /locus_tag="VLA60_11265"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HSF09984.1"
                     /translation="MAEAQILNGVNVAAVNELVKNVESDPKLGECKFHIKNTWSTCGQ
                     NQSKVSSFYAAKQEIPHDDPFTLNADEPAILAGHDTGANPVEHLLHALAGCLTTTLVY
                     HAAVRGIKIDALESELEGDLDIRGFLGLSNTVRSGFENIRVNFKVKTDAENIEKLKAL
                     SKLSPVFDMTSHGT"
     gene            complement(1170..1673)
                     /locus_tag="VLA60_11270"
     CDS             complement(1170..1673)
                     /locus_tag="VLA60_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09985.1"
                     /translation="MKKWGWLVQYGGTIILALGFGALLSQVPLFYETTLGTTKLRASQ
                     GVQFLGFAGALIVFWQLGQRTSNELSPLVPNLRFLCPVIMPLTTLIVLTVSHPVAGLV
                     LNPFLGKSHKIMYNWVFVLGIVSAALWVVIAWYVKAAPLLQQQDKSTKMSATGLPLSS
                     QEFHSLS"
     gene            complement(1764..2312)
                     /locus_tag="VLA60_11275"
     CDS             complement(1764..2312)
                     /locus_tag="VLA60_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSF09986.1"
                     /translation="MSGSNIVMSKWVICVGAVLGGILTISTWAQAADPQPQKPWNVGG
                     TMGFLANTPDDTAFALNFNADANVNRHIAVGPLLQLGFTGDLTLVGLSGQGKYAIPLP
                     ELGERFKVILQGGIGFVHADIGPSDTSFLIPIGIGVDYQINPQLAFKSDFLLNFTDLE
                     LGRGRGHNDTNVMPGLTFGVRF"
     gene            complement(2390..2821)
                     /locus_tag="VLA60_11280"
     CDS             complement(2390..2821)
                     /locus_tag="VLA60_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09987.1"
                     /translation="MKQHSILKKDLQLLVVGGVLGGLMIGCSSPQSVGIQIGESERTH
                     YPVVHKQPGPPPHAPAHGYRKKFAYQYYPTANVYYDQSRRVYFYLSGSEWKMAVSLPS
                     SLRIDVRETVSLELETDRPYMENDKHIKQVKYKEKGPKWKR"
     gene            2991..3257
                     /locus_tag="VLA60_11285"
     CDS             2991..3257
                     /locus_tag="VLA60_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09988.1"
                     /translation="MDKKKTSAACGRIVVEEIESELEGDLDIRGFLALSKTVRSGFQN
                     IRVNFKVKADAENIEKLKALSTRSPVFDMTSHGTNVQVKIKINK"
     gene            complement(3297..3956)
                     /locus_tag="VLA60_11290"
     CDS             complement(3297..3956)
                     /locus_tag="VLA60_11290"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSF09989.1"
                     /translation="MKNHRRRSDKIFLLLHSEQDKPFVGRLHRHLWDLGVTVLADGYE
                     ISRRHILEDYIDHAIKRDEIIGVVLSPNCINSRWCQRDLKQIIGAEKRSLRTRIIPLL
                     IGSVALPSFLMGYKYFNFSRSYYKTLTQIVAFYNQIEIPNMTWQHGNFRPKKLDHIKQ
                     FFEQAGWEILPAFTWLDYLKGLEELDQKGVHINRGFAKEGDFPQKMVPKEYVQLFSSF
                     T"
     gene            complement(3957..4169)
                     /locus_tag="VLA60_11295"
     CDS             complement(3957..4169)
                     /locus_tag="VLA60_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09990.1"
                     /translation="MVKPESGSLDMGACLLRKNIGKLGMGKPYARFDEGALGYPLSAV
                     VRSSRVRRNVREQLQVNTSALLYPFS"
     gene            complement(4762..5061)
                     /locus_tag="VLA60_11300"
     CDS             complement(4762..5061)
                     /locus_tag="VLA60_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HSF09991.1"
                     /translation="MGLHNPPHPGRSIRRDCLEALGLTVTDGAKILGITRHMLSRIIN
                     GQAGVSPEMAVRLEKAFGGTAQSWLALQQAYELAQVERGKIKVRSVSSVREEAKV"
     gene            complement(5154..5393)
                     /locus_tag="VLA60_11305"
     CDS             complement(5154..5393)
                     /locus_tag="VLA60_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09992.1"
                     /translation="MLTGVHEECTLKRMIVSIKHRGLKRWFYKEDPSGLTPHLVPIID
                     DILARLNVAEDVKAMSLPGWNLHPLKRGVERVLEY"
BASE COUNT         1631 a         1640 c         1553 g         1588 t
ORIGIN      
        1 aatacggttc atcaggattt tccgtgtgtc actcccattc tcgtgctcct ctttcgccat
       61 gaagcggcct taaattcctc tcctttgtaa ggagagggca agtgaggtag agtccgtgag
      121 ttatcggtca accaccactg gatcaaacat ggaaggctcc gcagaggcta cctccccttt
      181 gcccctcctt acaaaggagg gggacactgg aacaatcaac ctgaatattt acccatggga
      241 atgtcggaag acccccaaat attaacaata gggttgacta attcatggag gtgaatggcc
      301 atggctgaag cgcaaatcct taatggtgtc aatgttgcag cagtgaacga gctggtgaaa
      361 aacgtcgaga gtgatcccaa actgggtgaa tgcaagtttc acatcaaaaa cacctggagt
      421 acgtgtgggc aaaaccaatc gaaggtctca agcttttatg cggccaagca ggaaattcct
      481 cacgacgatc cattcaccct gaatgctgat gaacccgcca ttctggccgg gcatgatacc
      541 ggtgcgaacc ccgtcgagca cctgttacat gcgctggcgg gctgcctgac gacgaccttg
      601 gtgtaccatg cggccgtgcg aggcattaaa attgacgcac tggaatcgga actcgagggt
      661 gacctggata tcaggggatt ccttggtctc tcaaataccg tcaggagcgg gttcgaaaat
      721 attcgcgtca actttaaggt caaaaccgat gcggagaaca ttgagaagct taaggcatta
      781 agtaaactct caccggtgtt cgacatgacg tcccatggca cctaggtcca ggtgaatatc
      841 gagagaaagg gagtgttgaa aaaggccgcc agcagcgttc tcgccccttg gccgtgctca
      901 cgtactcctt cgtacgctcc gctcgtccaa gcggctgcgg ccttgctgga cggacatttt
      961 tgaacactcc actatttttc tctggtgagc ttcgttgact cctaagctga tgaagtggcg
     1021 aaatttttcc aatatacaac agtcccagaa aataaacagt cgaaagatat tggagcgatt
     1081 gaaagcaggc accggcggcg gatggggcgc gacaagccct tgcccctcat ttgtgaattg
     1141 tttgtgacat gatcaaaccc atttgcaagt cacgagagtg agtggaattc ctgtgaagaa
     1201 agaggcaatc ctgtggccga catttttgtg ctcttgtcct gttgttgaag gaggggagcg
     1261 gctttgacat accaggctat caccacccat agggcggcac tgacgatgcc aagtacaaac
     1321 acccagttat acatgatctt atgggatttt cccagaaatg gattaagcac gagaccggcg
     1381 accggatgac taacagtcaa aactattagg gtcgttaatg gcatgatcac cggacaaaga
     1441 aatctcaaat tcggcaccag gggggaaagc tcatttgacg tccgttgtcc caattgccaa
     1501 aacacaatta aggccccggc gaagcctaaa aattggaccc cctgcgatgc tcgcaattta
     1561 gtagtgccaa gggttgtctc atagaacagc gggacctggc tcaacagcgc tccaaacccc
     1621 agggcgagga ttatcgttcc accatactgc accagccatc cccatttttt catcgtatct
     1681 tttcctttct cgacacaccc gtttcagagt gcttattcaa catggcggca ttcagcaggc
     1741 ttgtaaccta gggacggaga tcatcaaaac cgcacgccaa aggtcaggcc gggcatcaca
     1801 ttggtatcat tgtggcctcg cccgcgcccg agttcgaggt cggtgaagtt cagaagaaaa
     1861 tcacttttga aggctagttg aggattgatt tggtaatcca ccccaatgcc tatggggatg
     1921 aggaacgagg tatcggatgg cccgatatcc gcatgcacaa atccaattcc accctgaagg
     1981 atgaccttga aacgctcgcc caattcgggc agaggaatag catatttacc ttgtcccgat
     2041 aatcccacga gagtgaggtc accggtaaac cccaattgca ggagcggtcc aacggctatg
     2101 tggcgattca cgtttgcatc ggcattgaaa tttagggcaa aagccgtgtc atcgggagta
     2161 ttggccagga atcccatcgt gccaccgaca ttccagggct tttgaggctg tgggtcagcg
     2221 gcttgcgccc aagtggaaat ggtaagaatg ccccccagta ctgccccgac acagatgacc
     2281 cacttcgaca tcactatgtt tgacccgctc atgtctcgaa tctcctttca cattaatccg
     2341 gctaataata ctttgacact tgattgcacc tttatgactt tccttcctac taccgtttcc
     2401 atttggggcc cttttctttg tatttgactt gtttaatgtg tttatcgttc tccatatagg
     2461 gtcggtcggt ttcaagttcc aaggagacgg tttcccgtac atctatgcgg agagaggagg
     2521 gcagggacac cgccattttc cattcgctgc cggaaagata aaaatacacc ctccgcgatt
     2581 ggtcgtaata gacattggcc gtgggataat actgataggc aaacttcttc cggtagccat
     2641 gagcgggagc atggggcgga ggtccaggtt gcttatgaac gacggggtaa tgggtccttt
     2701 cggattcacc aatttggata cccaccgatt gagggctgct gcaaccaatc atcaatccac
     2761 ctagcacccc ccccaccacc aataattgaa ggtctttttt taaaatcgag tgttgtttca
     2821 tcgggtcgta tctccttatt aaagaatggg cgatacctgt gagattagtt gtcgtcggaa
     2881 gggtccaaat ctgtagggcc ccgacaaaat gatttttgaa agcaaccggg gaaaaattag
     2941 aagaagagtg aagaacggct gtagaggacc taaaacatca acaaatttat gtggacaaaa
     3001 aaaagacgtc agcggcctgc gggaggattg tggtggagga aatcgaatcg gaactcgagg
     3061 gcgacctgga tatcagagga ttccttgccc tctcgaaaac ggtcaggagc gggtttcaga
     3121 atattcgcgt caattttaaa gtcaaagccg atgcggagaa cattgagaag ctcaaggcgc
     3181 tgagtacacg ctcaccggtg ttcgacatga cttcccatgg caccaatgtc caggtgaaaa
     3241 taaaaataaa caagtagttg taggacgtga atttgttttt taaaatgaca agtgcatcac
     3301 gtaaaagagg aaaatagctg gacgtattct ttaggcacca ttttttgggg gaaatcacct
     3361 tctttggcaa atcccctatt aatatgaaca cccttttgat ccagttcttc aaggcctttg
     3421 agatagtcta accaggtgaa cgcgggcagg atttcccatc ccgcctgctc aaaaaactgt
     3481 tttatatgat caagtttttt tggtctaaaa ttaccgtgct gccaagtcat gtttggaatt
     3541 tctatctgat tataaaaggc aacaatttgg gtcaaagtct tatagtagga gcggctgaaa
     3601 ttaaaatact tatagcccat taaaaatgac ggaagggcaa ccgatccaat cagcagcgga
     3661 attatgcgcg tcctcaatga tcgtttttct gccccaatta tttgttttaa atctctctgg
     3721 caccagcgcg agttaatgca gtttggggag agaacgacgc caataatttc gtccctttta
     3781 atggcatgat caatatagtc ctccaggatg tgtcgcctgg atatctcata cccgtctgca
     3841 agtacagtaa ctcccaaatc ccacaaatgc cggtgcaatc taccgacaaa aggtttgtct
     3901 tgctcactat gaaggagcaa aaatattttg tcagaccttc ttcggtgatt cttcatttaa
     3961 gaaaatgggt agagtagagc actggtgttc acctggagtt gttctcgaac gtttcgacgc
     4021 acgcggctac tccggaccac agcagacagg ggatacccca gcgccccctc atcaaaccgt
     4081 gcgtatggtt ttcccatacc cagctttccg atgttcttcc tcaggaggca tgcgcccata
     4141 tccagcgagc cggattcagg cttgaccatc cgaggcaggt tcctctgtag tgtcctgatc
     4201 gtgaatgggg ttgatagcag gaaggcaatc ccctgatcct ccactcgtcg gaaacgtgaa
     4261 caaagtaggg tcccctccct cgggcaaggt tctgttgtcc ttcgccctcg gtcagtacta
     4321 tgaaccactc cgactcccac gacagccctt gacgattccg gtgtccctta ctgaccctca
     4381 acctttggtt ccgttggttc caaaggagca aagcgactga agcgggcaga gttaggaccc
     4441 gtattactag gtgtcttagc ttgtgagagg cttccaccgc gcctgaatcg tatcggcaga
     4501 aaggtcggct ccacagtccc cttaatataa taccgcccat tgtgaatatg ctcgaatgtg
     4561 tttcgatctt tcaacggagc gaacgcttct gcttccagat aaggtgtgat gtcaaacaga
     4621 ccggcttgcc catcatcagc tttgatgtag agtaggtgat cgtcttttgg aataactgct
     4681 gtaatttcca ctgaaggaag ggcatacctt ccatttttcc cctgcttcca ttctcatgga
     4741 aaaaccatac aacagatttc tctaaacctt tgcttcttcc cgtacggagg acaccgagcg
     4801 aactttgatt tttccccgtt ctacctgtgc gagttcatac gcttgctgaa gcgctaacca
     4861 gctttgtgct gttccgccaa acgccttttc caagcgcacg gccatttccg gtgaaacgcc
     4921 ggcctgccca ttaataatcc tcgacaacat atgccgggta atccccaata ttttggctcc
     4981 atccgtaacc gtcaatccga gggcttcaag gcagtccctt ctgatggagc gaccgggatg
     5041 aggtggatta tgcagtccca taccatactc ctttctagtg gtaatcaacc aaattcacat
     5101 ccaggacctt gccgtcttca aaacgaaaaa taatgcgcca gtttcctgta accttaatac
     5161 tccaaaaccc tttccactcc cctttttaac ggatgcaggt tccatcccgg aaggctcatg
     5221 gccttcacat cctcggccac attcaaccgc gccaggatat cgtcaatgat cggcaccaga
     5281 tgcggggtta agcccgatgg gtcttcctta taaaaccacc gtttcagacc ccgatgctta
     5341 atactgacaa tcattctttt tagtgtgcac tcttcatgca caccagtcaa catgactccc
     5401 atcgtatggt caaaaactca agacccccga catggtcacc ttcattaaaa ttacacgctg
     5461 aactcaatgc acggggggat cgtcatgggg ctgttgaaaa tttcaaaaat tttcccattt
     5521 cctctgcata tgaatgcccc cgacccactc ctcgatggca atgagtggct ctgtcttacc
     5581 tgctccttga taggccttca tatgatcatc tcctcattga aacacccagc cgaatccgac
     5641 gataggcatg gggcagctgg gtggctcgca tatgctggcg taccatcgcc gaaaccaata
     5701 agccaggaat ctgtttcaag gacgattcac gtcaattcca gtgagaagga ggcgctctcg
     5761 ctcgaaccct gccgctacgt ggcacttaat cccttccggg caactatggt gacacatccc
     5821 cgatagtggg cgtggagccg ctatcccgaa acggtggggg cggctcgtga tcacgcctgg
     5881 ctgacaactg accagattac gaacagtttg gaaagcaagc ggggcctgtt caggagtgcg
     5941 atggaacctt cgtggccaag gaccacttgg gcttcgagac attccttgtc ggcagacgca
     6001 ggcgtagcgg gcaattttaa tcttcttggt tcacccacac ggcgagcagt catgacagat
     6061 tcactcgcgt ttcaatagta tcggttgatt ttggattacc gattatgggg gcgtgtatgc
     6121 cgcgaggata cgtggcctat caattcaggc gaaatcggct gatctaggat ggcaagacct
     6181 gacattcttt ccgattgatt ggcagacctt aaaacccggc ggtgcgccag ccgaatagca
     6241 atatgtattc ggctggcgca aacgctatgc gaggcgcgct tacctctggg tagacggctg
     6301 agagaaaatg gaaatcttgt ccggcgtctg ccccggcgtg ctgcgggttt ccattaccac
     6361 tggcgcggta aaagtggcag tgccttctaa cagaatatca gccacgagcg cc
//
LOCUS       DATIAL010000367         5560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1033990, whole genome shotgun sequence.
ACCESSION   DATIAL010000367 DATIAL010000000
VERSION     DATIAL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033990"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1572)
                     /locus_tag="VLA60_11310"
     CDS             complement(157..1572)
                     /locus_tag="VLA60_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF09993.1"
                     /translation="MSIIADTLQRLQTQTKSEGTETTQQAPLVLPARVRREPGWHRRP
                     SPLKFWLIGIGMAIGLSSLGMGAYWIGLHLDFGMPTEASSLPRQRVALIHSPPLSETP
                     PADSVSLEATQIPTVDPVESLPVSVAQEPDEESPAPQGDASMEPSSPLKVEQTFPTQP
                     VGEAPALQAPATPLIPPTSEDPQQKTGGTSLSTPHTTTDVAPNHPSTDPKKIASSPPV
                     TGLAPTEQLDSDFVEVASVNETGTPIPLEADLEEEKINLDELTTTTSLVIDEPSVPVN
                     TTALTLKNEGFQVNSQNTGPPQQSPANWLHRARELIQNGEYKEAQAMLSPLFHDPPVT
                     WEPWFWMGTAYLGAGQLEQADQYFLSGLARNDKVPQLWIQRALVAQQQGSFQLAVHEL
                     RQAEALQPDLPHIHLNMGYAFERMGNNRLANQYFGRFLQLTEGQPDFFSIRKKLLASL
                     TSITKTRGSTMGTPPSLRKEQ"
     gene            complement(1601..2425)
                     /locus_tag="VLA60_11315"
     CDS             complement(1601..2425)
                     /locus_tag="VLA60_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF09994.1"
                     /translation="MSTLDLSLCYQELGFSEPPFRLTPDTDFFFPGSHHLEALNHLRF
                     GIASGGLTMLTGEVGLGKTLLCRHLLRHPPTGVRFAYLMNPDQSYSDLLVSIYEDLTG
                     TLPEDQSLGALQRELPKLLLRLAVEGERVVVLVDEAHRLSGKVLEGLRLLSNLDTEKD
                     KLMCLLLVGQPELEQKLATRAFRPLRQRISVRYRLESFTWQETRAYIQHRLHIADSRH
                     RFHVGAGVLWLIYAWSRGVPRRINQLCDRALLAAYAQGGHTVTPMMIWRATKEFLP"
     gene            complement(2428..4026)
                     /locus_tag="VLA60_11320"
     CDS             complement(2428..4026)
                     /locus_tag="VLA60_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSF09995.1"
                     /translation="MRHNTIVIPQTSMISSRPSNGAFITCRARVFRSLALLGMFSLWG
                     CTVANPDADRSISDANTTQTSQRLSLSSDLAAPSVNNPQGTFSLEDRMLLTSDLPKPP
                     TPPPAPSLGPLYSFRAQDLPIIDALALFARSNHLNIVAGPDITGTITVDFHHLPLEQA
                     MSAILEAYGYYWEHHQNLIQVRQFKSKTMNIDYIRLVRGGAGQNRAQLNSGSSGSGSG
                     GTQDAGQITVNQQDEIKFWEELEKQIQTLMSKDGRLVLNRLSGTIQITDWYKRVDEID
                     YFLKKVHQALYRQVEIEVRIYEVNLNDNYSLGIDWSKINFLGSSGNIAIANIIAPPFV
                     PFGGFAFKAATTTISFADGSFDGVLKALQEQGDLRVVSQPRVVTLNNQPALIKVATDQ
                     PFFTQTIAQGTSGTGNIVTEQARSVTVGLVLSVTPQISEDGWIMMDITPIISRLRDIA
                     TSPNETSTAPVLDVKQSSGLVRLKNGEMVIIGGLIQEESSETERKVPILGDIPYLGRL
                     FKGTYTVKTKSELVIFLTPKIVQS"
     gene            complement(4055..4570)
                     /locus_tag="VLA60_11325"
     CDS             complement(4055..4570)
                     /locus_tag="VLA60_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09996.1"
                     /translation="MDENQRFRRALKNPLVAIGLCLLAGFAVYTNIVDTASDAPGIIS
                     VGLNRLLSAPTLPPLTRTGRPHDDEAGQWIEYASRDPFAPIVAASQVQPDPASMGADQ
                     TEEKQVTNTFTLKAIAIEAEQRSAVINRTVVYEGEMINGHQVLSIQPKGVWLKHQGKT
                     QWLTFSKKATS"
     gene            complement(4537..5124)
                     /locus_tag="VLA60_11330"
     CDS             complement(4537..5124)
                     /locus_tag="VLA60_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09997.1"
                     /translation="MKWLRRFEWPNPQQLKSTALVVLLIGTLSIVGWVSLDKLFNIQK
                     RQSLTQDAIGLEQQVSELRSQYLQARPDALDTHLQLAEQRLLHNFTHLAQWAQTLQID
                     GKQWNLLIQYRILSTERITSPIQGVTLVPLEIQVNSQGNPHAYRSYLQFLKALTRSGP
                     RVDIQNVTVLGDGQQATHLTIGLSVWMKTNDSVEL"
     gene            complement(5121..>5560)
                     /locus_tag="VLA60_11335"
     CDS             complement(5121..>5560)
                     /locus_tag="VLA60_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF09998.1"
                     /translation="ARHRTAIQTMTDQVTALQQDHAEWESRLMSLDTKRQWAQSVSET
                     TTPELEGPFLSYLGSIIPTQVILRKAFVERTQDGWNVELDGSTSTNLAATLLVVDQFV
                     QQLADGPYHVSLHEDWRDQLLTQTATSSNERPLYRFTLKGTIS"
BASE COUNT         1270 a         1329 c         1567 g         1394 t
ORIGIN      
        1 acattcaaag ggaggacgtc tagtcctgcg tcccgaggga gtcgaatgaa ctttgcgcat
       61 ggtgaatcgg ccccaacctt aatgaccgcc ctgggcagtg aaacctccag gaatatggtg
      121 gcgagagcat cttcaggcgc cagcgtattc cgtttgttat tgctctttcc ttaggcttgg
      181 aggggtgccc attgttgagc cccgcgtctt ggttattgag gtcaagctgg ccaacagttt
      241 ttttctgata gaaaaaaaat ccggctggcc ttctgttaat tgtaaaaatc taccgaaata
      301 ttgattcgct aaccgattat ttcccattcg ctcaaaggca tagcccatat tcaaatggat
      361 atggggtaaa tccggttgga gtgcctcggc ttgccgaagt tcatgcaccg caagttggaa
      421 gctcccttgt tgctgggcca ccagagctcg ttggatccac aattgcggaa ctttgtcgtt
      481 acgtgcgagg ccgctcaaaa aatactgatc agcctgctca agctggcctg cccccaaata
      541 tgccgttccc atccaaaacc atggttccca agtgacggga ggatcatgga agagggggga
      601 aagcatggct tgtgcctctt tgtattcacc gttttggata agttcgcggg cgcgatggag
      661 ccaattggct ggagattgct gaggtggacc tgtgttttga gagttcacct gaaacccctc
      721 gtttttcaga gtcagggctg tcgtgttaac agggacagaa ggctcgtcaa tcaccagaga
      781 cgtggtggtt gtgagctcat ccagattgat tttttcttcc tctaaatcgg cttctaacgg
      841 gatgggtgtg cctgtctcat tgacggatgc gacttcgaca aagtccgaat caagctgttc
      901 tgttggggca aggcctgtga ctggaggact gcttgcgatt ttcttcggat cagtgctcgg
      961 gtggttcggt gctacgtctg ttgttgtgtg tggcgttgag agggatgtgc ctccggtttt
     1021 ctgctgaggg tcttcggatg tggggggtat taatggtgtt gcaggtgcct ggagggccgg
     1081 agcttctccc acgggttgcg tggggaaggt ctgttcgact ttcaatggag aggatggctc
     1141 cattgaagca tccccttggg gggcgggcga ttcctcatcg ggttcctggg caacagatac
     1201 tggaaggctc tcaactgggt cgacagttgg tatctgcgtg gcttccagag aaaccgaatc
     1261 agcaggtgga gtctccgaaa gtggagggga gtgtataaga gccacacgct gtctgggcaa
     1321 agaagaggct tccgttggca ttccaaagtc cagatgtaag ccgatccagt aggcacccat
     1381 gcctagactg ctcaatccga ttgccatgcc gatcccgatc aaccaaaact ttaatggaga
     1441 gggccggcgg tgccaccccg gttctcttct cactctggct ggaaggacaa ggggtgcctg
     1501 ctgggtcgtc tcagtccctt cacttttggt ctgtgtctga agtctctgga gggtgtctgc
     1561 gatgatactc atggtctgtt ggttttccgt gatcctctca ctacggaaga aattctttgg
     1621 ttgcgcgcca gatcatcatg ggagtcaccg tatgtccgcc ttgagcatac gcggctaata
     1681 gagcccggtc gcacagttga ttgatgcggc gtgggactcc acgactccaa gcataaatga
     1741 gccagaggac tcccgcacca acatgaaatc ggtgtctgga atcggcgata tgcagtcggt
     1801 gttggatata cgcacgagtc tcctgccagg taaatgattc aaggcgatag cgaacgctaa
     1861 tgcgctgccg caatggtcga aaggctcgag tcgccaactt ttgttccaac tccggttgtc
     1921 caaccagcag gagacacatc aacttatctt tttcggtatc cagattggag agaagccgaa
     1981 gtccttccag gacttttcca ctgagccggt gggcttcgtc taccaatacg acgacccgtt
     2041 ctccttccac ggccagacgg agcaacagct taggcagttc ccgttgtaag gccccaaggg
     2101 actgatcctc aggcagggtc ccggtcaaat cttcataaat ggatacaagt aaatcagaat
     2161 aggactgatc cggattcatg aggtaagcga accgtacccc ggtcggcgga tggcgaagca
     2221 ggtgccggca caacagggtt ttgccgagac ccacttctcc tgtcagcatg gtgagcccac
     2281 cactggcaat gccaaatctg agatggttca acgcctccaa atgatggctg ccggggaaaa
     2341 agaaatcggt atccggcgtc aggcgaaatg ggggttcaga aaaccccaac tcttgataac
     2401 agaggctaag atcgagtgtc gacatgatca agactgtaca atcttgggag ttaaaaagat
     2461 gactaattca cttttggttt tgacggtgta cgtcccttta aacaggcggc caaggtaagg
     2521 aatatccccc agtatcggaa ccttgcgttc agtttccgat gattcttctt ggatgagacc
     2581 accaatgatc accatttctc catttttgag gcgaacgagc ccggaggatt gtttgacatc
     2641 taaaaccgga gcagtagagg tttcgttagg ggaggtggca atgtcacgaa gccgggagat
     2701 aattggagtg atatccatca tgatccaacc atcttcggaa atttgaggtg tgacggaaag
     2761 gaccaatccc actgtcaccg accgagcttg ctcggtaaca atattgccgg tgcctgaggt
     2821 cccttgggca atggtttggg taaaaaatgg ctgatccgtg gcgaccttaa ttaatgctgg
     2881 ttgattgttg agggtgacca cccggggttg ggacactacg cgcaggtcac cttgttcctg
     2941 gagagctttg aggacgccgt cgaagcttcc gtcagcaaag gaaatcgtgg tggttgccgc
     3001 cttaaaagca aaccctccaa aggggacgaa ggggggggca ataatgttcg cgattgcgat
     3061 atttccagat gaccccaaaa aattgatttt ggaccaatca atgcccaaac tgtaattgtc
     3121 gttaagattg acttcgtaaa ttctgacctc aatttcaact tgccgataga gagcttgatg
     3181 cactttttta aggaagtaat cgatttcatc aactcgcttg taccagtcag taatttgaat
     3241 cgttcccgaa agacgattaa ggaccagacg tccatccttc gacatcaagg tttgaatctg
     3301 cttttcgagt tcctcccaga atttgatttc gtcctgttgg ttgacagtaa tttgtcccgc
     3361 atcctgggtt cctcctgaac cggagccgga tgacccggag ttgagttggg cgcgattctg
     3421 tccggctcct ccccggacga gtcgaatgta atcgatattc attgttttgg atttgaattg
     3481 cctgacctgg atcaggttct ggtggtgctc ccagtaataa ccataggcct ccaaaatggc
     3541 cgacattgcc tgttccagag gcagatgatg aaaatccacg gtaatggtgc cggtgatgtc
     3601 tggtccggcc acaatattta aatggttgct tcgtgcaaac aatgccaatg catctatgat
     3661 cggtagatct tgtgcgcgaa acgaatacaa gggccccaaa gaaggggcag gcggcggtgt
     3721 cggaggtttg ggaagatcgc tggtgagcaa catgcgatcc tccagggaaa atgtcccctg
     3781 aggattgttc acagatggtg ccgccaaatc tgagctcagg gaaagtctct gtgaagtctg
     3841 tgtggtgttg gcatctgaga ttgaccggtc tgcatctgga ttggcgacgg tacatcccca
     3901 taggctgaac atacccaaaa gagccagact tcgaaaaacg cgcgcgcgac atgtgataaa
     3961 tgctccgttg gacggtcgac tggagatcat ggaggtctga ggaatcacta tggtgttgtg
     4021 gcgcatgtcg tctttcatag agtgatgagg gtagttatga cgttgctttc ttggaaaatg
     4081 ttaaccattg ggtcttgccc tgatgtttga gccacacgcc tttgggttga atggacagga
     4141 cctgatgccc gttaatcatt tctccttcat acacgacggt ccggttgatt accgcactcc
     4201 gttgctcagc ttcgatggca atggctttaa gggtgaacgt attggtcacc tgcttctcct
     4261 ctgtctggtc cgctcccatc gaagccggat ccggttgaac ctgagaggcc gccacgatgg
     4321 gggcaaaagg atcacgactg gcgtattcga tccattgacc agcctcatcg tcatggggcc
     4381 gccctgtacg ggtaagggga gggagagtcg gggcggaaag cagtcgattc agtcccacgg
     4441 agatgattcc cggtgcatcg gatgccgtat ccacgatatt ggtatagacc gcgaagcctg
     4501 cgagaaggca cagaccaatc gcgaccaacg gattttttaa agctcgacgg aatcgttggt
     4561 tttcatccat accgacaatc caatggttaa atgagtggct tgttgaccat cccccaggac
     4621 cgtcacattc tgaatgtcga ctcgtggtcc ggatcgggtc agggctttta ggaactgaag
     4681 ataggaacga tacgcatggg ggtttccctg cgagttgacc tgaatttcca acggaaccaa
     4741 tgtcactccc tggatgggag aggtgatgcg ttctgtgctc agaatccgat actggatgag
     4801 gagattccac tgtttcccgt cgatttgaag cgtttgcgcc cattgcgcca aatgggtgaa
     4861 gttgtggagt aagcgttgct cagccagttg gaggtgagtg tcaagcgcat ccggccgagc
     4921 ctgaaggtat tgagagcgaa gctccgacac ctgttgttcc aagccgatcg catcttgagt
     4981 caacgattgg cgcttctgaa tattgaaaag tttatccaga ctcacccagc ccacaatgga
     5041 taacgtgcca atcagtagca ccacgagtgc cgtgcttttg agctgttggg gattgggcca
     5101 ctcaaaacgg cgaagccact tcatgatatg gttcccttca aggtgaaccg atagagtggg
     5161 cgttcattgc ttgaggtggc ggtttgggtc aagagttgat ctcgccaatc ctcatgcagg
     5221 gacacatgat atggtccatc tgccagctgt tgcacgaatt gatccaccac aagcaatgtg
     5281 gcggcaagat tggtagaagt gctgccgtcc agctccacgt tccatccgtc ctgggttcgc
     5341 tccacaaagg ctttgcgcaa gatgacctgg gtggggataa tggatcccaa atagctgaga
     5401 aacgggcctt ctaattccgg cgtggtggtt tcgctgacgg attgcgccca ttgccgtttc
     5461 gtgtctaacg acatgaggcg actttcccat tccgcgtgat cctgctggag ggcggtgacc
     5521 tggtcggtca tggtctgaat cgcagtgcga tgtctggcca
//
LOCUS       DATIAL010000368         3306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_496779, whole genome shotgun sequence.
ACCESSION   DATIAL010000368 DATIAL010000000
VERSION     DATIAL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496779"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /gene="atpH"
                     /locus_tag="VLA60_11340"
     CDS             complement(<1..457)
                     /gene="atpH"
                     /locus_tag="VLA60_11340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HSF09999.1"
                     /translation="MNKSTIGRRYASALFQLLDKSGIEPARSALSALQQGLEETPALK
                     HVLASPVFNFEEKQAVLNELSQRMEAPPVMRDFLTQLLKKNRAMLLPEISEAFAELAD
                     QQQGVQQVWVGSAKPLQAAEKEQIKQDLSQTLHHGVEVAFEVDPHLIAGL"
     gene            complement(590..1741)
                     /gene="mnmA"
                     /locus_tag="VLA60_11345"
     CDS             complement(590..1741)
                     /gene="mnmA"
                     /locus_tag="VLA60_11345"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HSF10000.1"
                     /translation="MPVSDSKRVVLGMSGGVDSSVAAKLLKQQGYEVIGVTLKVWEEE
                     DDTSVSKRWQERGCCKVGMARHVAELLGISHRVIDTRERFQKGVIKDFIGGYLQGTTP
                     NPCVRCNERVKFGGLFQVADELGADYIATGHYANIKHDEGGTPFLVRGQDAKKDQSYF
                     LYRIPPSWLSKMMFPLGEMEKPDVWREAEAMGLPVEELKESQEICFVTQGDYRQFLKL
                     HAPHASSPGTFVDEKGKELGQHQGVAFYTPGQRKGLGIAGGSRLYVQRVLPDMNMVVL
                     GPAEDLDSRECLVSDLNILSPESLQHASKIDVKFRYSSPPVPATYRWPDSTSITIRFD
                     APVRALSPGQSAVFYLEDRVLGGGIIQGPTPKKNIQMCPEPNLAARPQR"
     gene            complement(1741..2217)
                     /locus_tag="VLA60_11350"
     CDS             complement(1741..2217)
                     /locus_tag="VLA60_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSF10001.1"
                     /translation="MWGEKEKKKELGFGQEFYTFLGKGVDFKGKAQFEGTVRVDGNFE
                     GEITIDDTLIIGESAVIKGTITGGTIVNSGRIEGVITAKSKIQLLKPAVLIGDVHTPI
                     FAMEEGVYFQGECDMGSAPKLEAPIDNKILSNGQGKTIPKVEQKLEKKLETEPLSR"
     gene            complement(2319..2819)
                     /locus_tag="VLA60_11355"
     CDS             complement(2319..2819)
                     /locus_tag="VLA60_11355"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSF10002.1"
                     /translation="MFFKKSEENTQDRAEMSQGQEKESAVVDSPVTSASQQESGDIIA
                     FVGEEVTFKGTIRYQGTVRVDGRLEGEIYTDGNLIIGQKAVIAAKIHAGTIMCQGQLT
                     GDVEAKHRVKLLSPAVFEGTITSPLLSMDEGVVFNGTCNMPQKMERKGKEAKLVISVE
                     EAVKVK"
     gene            complement(2976..>3306)
                     /locus_tag="VLA60_11360"
     CDS             complement(2976..>3306)
                     /locus_tag="VLA60_11360"
                     /inference="COORDINATES: protein motif:HMM:NF036800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10003.1"
                     /translation="YIEAGVLSLGHAKVLAGLPTPEAQIQLARKIVSEQLSVRQIEQM
                     ITVQRKGKGAKSKKEEPNRFRNLEEQFRKRLGTKVQVKAGVRGGQVIIHYFSEDELDR
                     IASLVLE"
BASE COUNT          795 a          895 c          685 g          931 t
ORIGIN      
        1 tcaatcccgc aataagatgg ggatctacct caaaagccac ttcgactcca tggtgcagcg
       61 tttgagacaa gtcttgcttg atctgttctt tctcggccgc ttgtaatggt ttggccgagc
      121 ccacccagac ttgctgaact ccctgttgtt gatcggccaa ttccgcaaag gcctctgaaa
      181 tttcagggag caacatggct cgattttttt tcaacagttg agtgaggaaa tcccgcatga
      241 ccggaggggc ctccatcctc tgactcaatt cattcaggac agcctgtttt tcctcaaaat
      301 taaaaactgg tgaagcgagg acatgcttca aggcaggggt ttcctccaag ccttgttgca
      361 atgcgcttaa ggcacttcgt gcgggttcaa tacccgattt atcgagcagt tggaaaagcg
      421 ccgatgcata tcggcgccca atggtgcttt tattcacagt ttacagagat tccaggcagc
      481 cacgtgttga acggaattgg gcgtgtgtta agcgggtgaa actagcatga gtgatacagc
      541 ctgtcaaccg gaaagagcgg atgtaaaggg aattttttct gattcccaat tagcgttggg
      601 ggcgtgcagc taagttgggt tccgggcaca tctgaatatt tttctttggg gttggccctt
      661 gaatgattcc acctcccaaa acacgatcct caagataaaa aaccgctgac tggccaggac
      721 tcaaagcccg aacaggggca tcaaacctta ttgtgatcga agtggaatcc ggccatcggt
      781 acgtagccgg gactggtggc gaactataac gaaatttcac atcaattttg cttgcatgct
      841 gcaatgactc tggagagaga atattcaagt ctgaaaccag gcattctctg gaatctaaat
      901 cttcggcagg acccaggacc accatattca tgtcgggaag cacgcgctgc acatacaaac
      961 ggcttcctcc ggcaataccc aatccttttc gttggcctgg ggtataaaag gccacgcctt
     1021 gatgctgccc aagctccttg cccttttcat caacaaacgt accgggactt gaagcatgcg
     1081 gtgcatgcag cttgagaaat tgccggtaat caccttgcgt cacaaagcaa atttcctggc
     1141 tttctttgag ttcttcgacg ggcaggccca tggcctccgc ttctctccag acgtcaggtt
     1201 tttccatttc ccctaaagga aacatcattt ttgagagcca actgggtgga attcgataga
     1261 gaaaatatga ttggtctttt ttggcatcct gcccacgaac caggaatggg gtccctcctt
     1321 catcgtgttt gatattggca tagtggccgg tcgcaatata gtcagctcca agttcatcgg
     1381 ccacctggaa gagacctcca aatttcactc gttcattaca ccgaacgcag ggattaggcg
     1441 tggttccctg gagatacccc ccaatgaaat ccttaatgac tcccttttga aaacgttcac
     1501 gggtatcaat gaccctgtga gagatcccta ataattctgc cacatgcctg gccattccca
     1561 ctttacagca tccccgctcc tgccaacgct tggaaacgga ggtgtcatcc tcttcctccc
     1621 agactttcaa tgtgaccccg ataacttcgt acccctgctg cttcaataat ttggcagcca
     1681 cagagctatc cacaccgccg ctcattccta agacaaccct tttggaatca gagacaggca
     1741 ttagcgactc aacggctctg tttctaattt tttttctaat ttttgctcaa ccttaggaat
     1801 cgttttgcct tgtccattgg agaggatctt attgtcaata ggcgcttcta attttggagc
     1861 ggaacccata tcgcattccc cttggaagta cacgccttcc tccatggcaa aaatgggggt
     1921 atggacatca ccaatgagaa ccgccggctt aaggagttga attttagatt ttgcggtaat
     1981 cacaccctca atccggccac tgttcacaat agttccacct gtgatggtcc ctttgatcac
     2041 ggcactttca ccaatgatga gggtatcatc aatcgtgatt tccccttcga agttaccatc
     2101 cacccgcacc gtgccctcaa attgagcttt gcccttaaaa tcaacacctt tccccaaaaa
     2161 tgtataaaat tcctgcccaa atcctaattc ttttttcttt tctttttcac cccacataca
     2221 tcgttcctcc tctcatccag taaagagccc tctagttgca cgctaggatc tccatgtcca
     2281 actgggaact agccgcctat tttttgcaga agattgcttt atttcacctt cacggcttct
     2341 tctaccgaaa tcacaagttt ggcctctttg ccttttcgct ccattttttg gggcatattg
     2401 caggttccat taaacaccac tccctcatcc atcgaaagaa ggggggaggt aattgtccct
     2461 tcaaagacag ctggggataa caatttaacg cggtgcttgg cttcgacatc ccccgtcaat
     2521 tgtccctgac acataatggt tcctgcatga atcttggcag cgatgaccgc tttttgccca
     2581 atgattaaat tgccgtcggt atatatttca ccctcaaggc ggccatccac tcgaaccgtg
     2641 ccctgatacc gaatagtgcc cttaaaggtc acctcttcac caacaaaagc aatgatatcg
     2701 cctgattcct gttgactggc tgaagtcacc ggactatcca ccacagctga ttccttctcc
     2761 tgaccttgac tcatttctgc cctatcctga gtgttttctt cgcttttttt gaaaaacatg
     2821 agcctctccc tcctcgataa ctgtgtgctc caattccttc atgcctgact cgatacccgg
     2881 taatattagt tcgaccattt gccatcatca tgatcctttg acatcttaaa ggatagccca
     2941 ttcgctcaga agccatccgt tggattattc aaattctatt ccaaaaccaa tgaagcaatc
     3001 cgatccagtt catcctcgga aaaataatgg atgatgacct gccctcccct gactccggcc
     3061 tttacctgga ctttagtacc caagcgtttt ctaaattgct cttccaggtt tcgaaatcta
     3121 ttgggttcct ctttttttga tttggcgcct ttaccttttc tctgcacggt aatcatctgc
     3181 tcaatttggc gaacagagag ttgctcggaa acaattttcc gtgcaagctg gatttgtgct
     3241 tctggagttg gcaggcccgc taataccttc gcgtgaccta aactgaggac ccccgcctca
     3301 atatac
//
LOCUS       DATIAL010000369        11817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1249664, whole genome shotgun sequence.
ACCESSION   DATIAL010000369 DATIAL010000000
VERSION     DATIAL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11817
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249664"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VLA60_11365"
     CDS             <1..192
                     /locus_tag="VLA60_11365"
                     /inference="COORDINATES: protein motif:HMM:NF018620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HSF10004.1"
                     /translation="LQDFVSAVETLSGEEDYQALVGHFGVSRNDPDFWKHSDALRIAY
                     QKESPIEAGLLDYNRLENW"
     gene            314..766
                     /locus_tag="VLA60_11370"
     CDS             314..766
                     /locus_tag="VLA60_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10005.1"
                     /translation="MLTLAQCTHGQIPEGKHTSTDLRILAQWSGNYPVIYLDQLPEGQ
                     TSGQAGYLGSPTQFRRVWSNFKPGEAVPQVNFDASLVVFTRNIAFYNHINILKITLKD
                     GVADIIAMETRSAQPIEDHVAMAMAAIPRKGIKYILAGQERVPISHAH"
     gene            756..1724
                     /locus_tag="VLA60_11375"
     CDS             756..1724
                     /locus_tag="VLA60_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10006.1"
                     /translation="MPIKRSCRVGMVIWNVEIMVFAGILTIFTVLNCSASPTPAQEPT
                     EIPAQEFATDPLNATYSIEGRTVRLQDGRSERSITPHSATTMKTVVYGKPVYGDLDGD
                     GQKDAAILLIHDPGGSGTFFYVAGALIINGAFRGTNAVWLGDRITPPDLQIRNGILIV
                     RYADRGHGEPMVTTPTVPMANYLILKNAQLESIVPLKEGEQIVEGWVTIGHEVRSFRP
                     CLEKKEHWLQGDSPGLSRILVAYKEAMPHPNLYLPLLMYTGRKNSHATGRGIRGRVRG
                     GISCHPTRSSHAGWNLQQGRPCDTFLGDQEGCGVFPLGAWRNEPLI"
     gene            1983..3083
                     /locus_tag="VLA60_11380"
     CDS             1983..3083
                     /locus_tag="VLA60_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF10007.1"
                     /translation="MANHSTPPRNEEFTKNAVEAAIRIGLVGLWVGWCFLILRPFVIP
                     IVWGLIIAVAIYPVYGSLRTLLHDSPRVSAILVTAGMLVILVWPAVLLGGILVKNGEM
                     VVNHLRDGTLTVPPPPQGLETWPLIGKPLANIWNLASVNIEGAFIQLEPQIRVLASSL
                     LSMVTGTGLAILQFFVAVVIAGVLLAYSASGYQLAHSIGRRLAGDRGTELATLAEATV
                     RSVARGVLGVALIQSLFAGLGFFIAGIPAAGFWAFLCLILGIVQIGVWPVMIPVVIYV
                     FSTQDTLTSVLFLIWSIFVSLLDNILKPIFLGRGLDVPMVVIFMGAIGGLLLSGIIGL
                     FFGAVVLTLGYKLFLAWLEVDQPTHEDKADKL"
     gene            3165..3929
                     /locus_tag="VLA60_11385"
     CDS             3165..3929
                     /locus_tag="VLA60_11385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF10008.1"
                     /translation="MKIDQGDKAVFVVVPALSPVLSCRLRYLEDKVEQTLIVHKKELA
                     MNQERKTHWEDVYRDTATEELGWYQAHPTLSLNLIQATGVQKTGSLIDVGGGDSTLVD
                     HLLDQDFNHITVLDISEVALERAKARLGDRADLVTWIEADITDFRSSRTYDVWHDRAV
                     FHFLTEAEDREKYCETMNRAVSAKGHVIMATFGYEAPPTCSGLPVVRYSPEFLTLAIG
                     NNFVCIESFEELHMTSSGNKQPFIYCRFTRRNPSHL"
     gene            complement(4026..5099)
                     /locus_tag="VLA60_11390"
     CDS             complement(4026..5099)
                     /locus_tag="VLA60_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HSF10009.1"
                     /translation="MFHSQTGVGALYGLGAAVLFGLSTPILKLFLPVYDLLPVVGLLY
                     TGAGIGLGFLEIFLKSTTSPASSQAETPVQTGDIGLLLGMILTGGILGPILMLYGLER
                     IPAVVGSLLLNLEAPLTILLAVTLFREHLGKREVGAAMCIFVGALLLSYGSGAIYGDW
                     LGVISVSGACLCWAVDNNLTQGLSLRDPLAVARIKGLSAGGFSLGLSWLMGYEFPDMT
                     AISMILVAGFVGYGISVVLDIYALRILGAAREAAFFATAPFIGALAAVPILGEHWSVA
                     EYLAGAAMAIGVSILLREHHNHLHVHTNMEHDHVHTHTHTDPHHQHQHEEEVVGSHAH
                     WHRHLPLQHDHGHVSDLHHRHEH"
     gene            complement(5089..5391)
                     /locus_tag="VLA60_11395"
     CDS             complement(5089..5391)
                     /locus_tag="VLA60_11395"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HSF10010.1"
                     /translation="MVGMIFKTLKTLGQKLTYEVSVYRLAFRDTRTPIFSKILLGLAI
                     GYLALPFDLIPDFLPVIGHLDDLIIVPGLVFLALKYIPVHIVEECRTQVKAESRVS"
     gene            complement(5453..5854)
                     /locus_tag="VLA60_11400"
     CDS             complement(5453..5854)
                     /locus_tag="VLA60_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF10011.1"
                     /translation="MDTIGVLSQKTGCHIETIRYYKRVGLLAKPPRTEGGHRLYGKEQ
                     LKRSVFIRRSRELGFSLEEIRTLFRLVDGKRYTCREVKGVTEQHLRDVSRKISDLRRL
                     QKILRSISSQCEGGMVPDCPIIESLFEEKRL"
     gene            6034..6246
                     /locus_tag="VLA60_11405"
     CDS             6034..6246
                     /locus_tag="VLA60_11405"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HSF10012.1"
                     /translation="MGVSTQQQSVIRCPQCGFQKTETMSLDSCQVRYECRKCGTILKP
                     KAGDCCVFCSYGTVSCPPQQTKHLDT"
     gene            6407..6652
                     /locus_tag="VLA60_11410"
     CDS             6407..6652
                     /locus_tag="VLA60_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10013.1"
                     /translation="MAKDDAKICAVPNVAWAMVKAGHQVTVLVDASAVTSVTKGFGWF
                     RHLVHSDTTALDGARLPEGERISLADRMGVPLEEIPH"
     gene            6665..6826
                     /locus_tag="VLA60_11415"
     CDS             6665..6826
                     /locus_tag="VLA60_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10014.1"
                     /translation="MAFLKKTGVTIYGNQNMMLLNNIDLDDVAPEVTPIALNRMVDFF
                     QSADHTIVY"
     gene            complement(6901..7290)
                     /locus_tag="VLA60_11420"
     CDS             complement(6901..7290)
                     /locus_tag="VLA60_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10015.1"
                     /translation="MHDGYFNAAEPAFNRTRCGLIRLLLDWIVLEFCNVPSSWANPNS
                     RNEEHSQQIGTFDTETVGTLPKNFVVGTTLNGRSAGVRKVIDMTQLLNLLHDLDPREH
                     TRIKNVLQVIAAPSPSYVLAQLKKLWF"
     gene            complement(7309..8052)
                     /locus_tag="VLA60_11425"
     CDS             complement(7309..8052)
                     /locus_tag="VLA60_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10016.1"
                     /translation="MSILSVLHKPTFASLTWLFLGIIFLGMATAGQFGTPKGEEGTRI
                     FKATEHPTYSGQFRLTGRDGMLVGSMGEKAPWDHMDYAGKHLVAIEGKIEIEVDEAAN
                     SGLVEAKFVEGNDQYRIVFARFAGLAPYQDGGLATRVYEHGDSGNGDSLYPKTWLYLA
                     GWGKADVFKNGELLFQDYAAHFMVMERSRDPNTHQVPYPTTRNLPGGETDPAGMEIDL
                     WVRSAETNTENFPPFETFIHLFWEEVTWK"
     gene            complement(8157..9521)
                     /gene="chrA"
                     /locus_tag="VLA60_11430"
     CDS             complement(8157..9521)
                     /gene="chrA"
                     /locus_tag="VLA60_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HSF10017.1"
                     /translation="MNKPDAHPVVSTASPPLPVTFWQAFVFWLKLGFISFGGPAGQIA
                     IMHQELVERRRWISERRFLHALNYCMVLPGPEAQQLATYIGWLMHRTWGGIMAGALFV
                     LPSLLILIVLSWIYIAFGDVPLVAGLFYGIKPAVTAIVVQAAQRIGSRALKNNVLWAI
                     AAASFVAIFALNVPFPTIILVAGLIGHFGGRIAPDTFRAGGGHNQTDKSVGLTLIDDD
                     TPPPEHAYFRWTKLLKVTVIGSVLWAVPMACLTVTFGWQHTLTQMCWFFTKAALVTFG
                     GAYAVLPYVYQGAVEYYDWLTPMQMIDGLALGETTPGPLIMVVAFVGFVGGYVKVLFG
                     PEFVFLAGAVAATLVTWFTFLPSFLFILAGGPLVESTHGDITFTAPLTAITAAVVGVI
                     LNLALFFGYHVLWPHGFTGAFDWVSALIALAAAVALFRYRRNVMQVIAVCALIGLIIK
                     SLIL"
     gene            complement(9836..11224)
                     /gene="zwf"
                     /locus_tag="VLA60_11435"
     CDS             complement(9836..11224)
                     /gene="zwf"
                     /locus_tag="VLA60_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSF10018.1"
                     /translation="MMGKARSDALVLFGATGDLAQKKIFPALQAMIQHGKVDVPIIGV
                     ARGGKDVDHLRQRARDSLEKLGGGVDETAFAKLSDLLRCVDGDYQEEDTFTRLRQTLA
                     TAQHPLFYLAIPPTLFPAVVESLGKSGCASGARVVVEKPFGRDLASAQALNDTLRSVF
                     DESAIFRIDHYLGKESIRNLLFFRFANSFLEPIWNRNYVESVQITMAERFGVEGRGKF
                     YEETGAIRDVVQNHLLQVVANLAMEPPVSSGGEALRDERVKAFKGIRPLTGKSLVRGQ
                     FRGYRDEAGVRPDSQVETFASMQLHIDSWRWEGVPFFIRTGKCLPVTATEVLVKLQHP
                     PHQLFDDTRSDASNHIRFRLGPDRVAIAMGARAKRPGERMIGEDIELYVCRQQGDEQG
                     AYERLIGDAMEGDASLFARQDGVEEAWRIVDPILKMSTPVFEYAAGTWGPNEAQAVVA
                     DIGGWHNPTEEG"
     gene            complement(11632..>11817)
                     /locus_tag="VLA60_11440"
     CDS             complement(11632..>11817)
                     /locus_tag="VLA60_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10019.1"
                     /translation="YAFKVGEHDFDRIFSRIQAEDIPYGSGPTAREDMTINHRGGGRG
                     VYFCDPNGHILELLTVG"
BASE COUNT         2948 a         3129 c         3023 g         2717 t
ORIGIN      
        1 ttacaagatt ttgtcagcgc cgtggaaacc ctcagcggcg aggaggatta tcaagccctg
       61 gtgggtcatt ttggtgtcag ccgaaacgat cccgatttct ggaaacatag tgatgcctta
      121 cggattgctt atcaaaagga gtctccgatt gaagcaggat tgttggacta taatcgtctg
      181 gaaaactggt aagaaggcgc gagcaacgaa aacatcgctg aaagatcttc cgacgccggg
      241 gcgttacatt ttccaaggag tgccggtgcc attcgcccaa agactcatct catgtctcgt
      301 tctgatgtgc gtcatgctga cacttgcaca atgtacgcat gggcagatcc ctgagggaaa
      361 acacacatcg accgacctcc ggatcctggc acaatggagc ggcaattatc cggtcattta
      421 cctcgaccaa ctcccggagg gtcagacgtc gggtcaggcc ggctatctgg gaagccctac
      481 acagtttcgg cgggtctggt caaacttcaa gcccggcgaa gcggtgcccc aggtgaactt
      541 cgatgccagt cttgtggtct ttaccaggaa cattgctttc tacaaccaca tcaacatcct
      601 gaagattacg ctgaaggatg gcgtggcaga cattatcgca atggaaacgc gatcggcgca
      661 gcccattgaa gaccacgtcg caatggcgat ggcagcgata ccacgtaaag gaatcaagta
      721 tatcctggcc ggccaagaac gcgttcccat atcacatgcc cattaaacga tcgtgtcgtg
      781 ttggcatggt gatctggaat gttgaaataa tggtcttcgc gggcattctc accattttca
      841 ccgtgctcaa ttgctccgcc tcccctaccc ccgcccagga acccactgaa attccggcac
      901 aggaatttgc gacagatccg ctgaatgcta catattctat cgaaggccgc actgtccgct
      961 tgcaagacgg tcggtcggag agaagcataa cgcctcattc cgcgaccaca atgaagacgg
     1021 tcgtgtacgg caaacctgtg tatggcgatt tagacggtga tggccagaag gatgcagcca
     1081 tactcctgat ccatgatcct ggcggaagcg gcacattctt ctatgtagcg ggtgcactca
     1141 tcatcaatgg cgcattccgg ggaacaaatg ccgtatggct tggcgatcgg atcaccccac
     1201 ctgatctcca aatccgcaat gggattctta tcgtcaggta tgccgatcgt ggccacggag
     1261 agccgatggt gaccacaccg accgtcccaa tggcaaatta cttgattcta aaaaatgcgc
     1321 aattggaaag tatcgtgccg cttaaagaag gtgaacagat tgttgaaggc tgggtcacca
     1381 ttggccatga agtccgctca tttagaccgt gccttgagaa aaaagagcat tggcttcagg
     1441 gggattcacc tgggctaagc cgaatcctgg ttgcctataa ggaggccatg cctcatccaa
     1501 atctgtacct gcctctgttg atgtatactg gccggaaaaa cagtcatgcc accggcagag
     1561 ggattcgggg cagagtacga ggcggcattt cttgccaccc aactcgttca agtcatgccg
     1621 gatggaacct gcaacaggga cgaccgtgcg atacattcct tggagatcag gaaggatgtg
     1681 gggttttccc actaggagcc tggagaaatg aacctttgat ctgactatcc tgaatccgaa
     1741 tgggctctat gaaaggctgt atgaaaaaca accccggcct tatgaatatt gcatttccgg
     1801 tatggaacgg tacaattcca atttcaaaca gattggttca agatggccga gcctgtccga
     1861 atcgccttga cgcatcggcg gtgagagcca cgaatttctt tgtatcggct caatcacttg
     1921 cacttccttt agggatcacg accccatact atttaaagta tcaggagaag gattagacac
     1981 atatggcgaa tcactcaact cccccccgaa atgaggaatt tacaaaaaac gcggtggagg
     2041 ccgcgattcg gatcggcctg gtcggccttt gggttggatg gtgtttccta atcctacgcc
     2101 ccttcgtcat ccccattgtc tggggtctca tcattgccgt ggccatctat ccggtttatg
     2161 ggtctctacg gactctttta catgattctc ctcgtgtgtc agcaatattg gtaacggccg
     2221 gtatgttggt gatcctggtt tggcctgcag tcctgttagg aggaattctt gttaaaaacg
     2281 gggaaatggt tgtgaatcat ttgcgtgatg ggacactcac agtcccccca ccaccgcagg
     2341 gtcttgaaac ttggccgttg attggaaaac cgttagccaa tatctggaac ctggcttccg
     2401 tgaatattga gggagccttc atccaacttg aaccccagat cagggtctta gcaagctcgc
     2461 tcctgtctat ggtcacaggg acaggtctgg caatcttgca attttttgtg gcggtggtga
     2521 tcgccggagt gctcctggct tactccgcgt ccggctatca gctcgcgcat agtattggca
     2581 ggcgcctggc tggagaccgc ggaaccgagc ttgcgaccct tgcagaagcg acggtgcgaa
     2641 gcgtggcgcg aggggtgttg ggggtggccc tgattcagtc gctatttgca gggttgggat
     2701 ttttcatcgc gggaatccct gcagcgggat tctgggcatt tttgtgtctc atcctgggca
     2761 tcgtgcaaat cggcgtctgg cccgtcatga taccggtggt catttacgtg ttctcaacac
     2821 aggataccct gacatctgtg ctgtttctga tatggtcaat ttttgtcagt ctgcttgata
     2881 atatcctcaa accgatcttt ttaggacgcg ggctggatgt tccgatggtg gtgattttca
     2941 tgggtgccat cgggggattg ctcttgtcgg gtatcattgg cttatttttt ggagccgtgg
     3001 ttttaacttt ggggtataaa ttgttcctgg catggctgga agtagaccag cctacccacg
     3061 aggacaaggc ggacaaatta tagaaaggta gcactccaca cccataccgg agtttgatca
     3121 aatttgtgaa aatgagatgc taggatacgg aatgtattct atgtatgaag atcgatcagg
     3181 gcgacaaagc tgtgtttgta gttgtgccgg cactttcccc tgtcctctcc tgcaggctga
     3241 gatatttgga ggataaagtc gagcaaacct tgatcgtcca caagaaagaa ttagccatga
     3301 atcaagagag aaagacacat tgggaagacg tgtatcggga cacggccaca gaagagttag
     3361 gctggtatca agcccacccc accctatcat tgaatctgat tcaggccaca ggcgttcaaa
     3421 aaaccggaag cctcatcgat gtggggggag gcgattcgac attggtagac cacctcctcg
     3481 atcaagattt caatcacatc accgtactcg atatttccga agttgcattg gagcgggcca
     3541 aagccagatt aggcgatcgc gcggatctcg tgacatggat cgaagccgat attactgatt
     3601 ttcgttcatc aaggacgtat gatgtctggc acgatcgcgc cgtgtttcat ttcctgacgg
     3661 aggcggagga tcgagaaaaa tactgtgaga ccatgaacag ggctgtgagt gcgaagggcc
     3721 acgtgatcat ggcaacgttt ggttatgaag ctccaccaac atgcagcgga ttaccggtcg
     3781 tccggtattc ccctgaattt ttgaccttag ctatcggaaa taactttgtc tgtattgagt
     3841 cgttcgaaga gcttcacatg acgtcaagtg gcaacaagca accgtttatc tattgccgct
     3901 ttaccagacg aaacccctca catctctagg ggtggagaat tttgaaatga ctatttgagg
     3961 gggatcaaaa agccccaaaa tgaaccagtc cacgatgtcg agaggcaatc ctttccagct
     4021 tcctgctaat gctcgtgtcg atggtgcaag tccgacacat gtccgtgatc atgttgcaac
     4081 ggcagatgcc gatgccaatg ggcgtgggac cccacgactt cttcttcgtg ttgatgttga
     4141 tgatggggat cggtgtgggt gtgggtgtgg acatgatcgt gctccatgtt tgtatgaacg
     4201 tggaggtggt tatgatgttc tctcaggaga atactaaccc ctatggccat cgccgcccct
     4261 gccagatact ccgcaacgct ccaatgttct ccgagaatag gcacggcagc cagggcgccg
     4321 ataaaaggag ccgtcgcgaa aaatgcggct tcccgggctg cgccgagaat gcgcaaggca
     4381 tagatgtcca acacgacact aattccatac cccacaaaac ctgcaaccaa aatcatactg
     4441 attgcggtca tatccggaaa ttcatagccc atcagccatg acaggcccag cgaaaatcct
     4501 ccggcactta atcccttgat ccttgccaca gccagcggat ctcgaagtga aagcccctgg
     4561 gtgagattgt tatccacagc ccagcataga caagccccac tcacgctgat gacgcctaac
     4621 cagtcaccat agatcgctcc agacccataa ctgagaagta aggcacccac aaaaatgcac
     4681 atagctgcac caacctcccg tttccccaaa tgttcacgaa aaagggtgac ggccagaagt
     4741 atggtgagag gcgcttccag gttgagcaag agcgatccga ccacggcagg aatccgttca
     4801 agcccataga gcatgagtat gggccccaaa attccgcctg tcagaatcat tcccagtaac
     4861 agaccgatat cacctgtttg aacgggtgtt tcggcctggg atgacgccgg ggacgtcgtg
     4921 gacttcaaaa agatttccaa gaaaccaaga ccgatgcccg cccccgtata taacaatccg
     4981 accacaggga gcaggtcata cacaggaaga aacagtttga gaatgggcgt tgacaggccg
     5041 aatagcaccg cagcgcccaa tccatacaga gcccccactc ctgtttgact atgaaacacg
     5101 actctctgct ttcacttgtg ttcggcactc ttcaacgata tgtaccggga tgtacttcaa
     5161 ggcgaggaac acgagtcccg ggacaatgat cagatcgtcc agatggccaa taacggggag
     5221 gaagtcagga ataaggtcga agggaagagc cagatacccg atggccaacc ctaacaatat
     5281 cttggagaag attggcgtcc ttgtgtcacg aaacgccagc cgatagacgc tgacctcgta
     5341 tgtcagtttt tgtccgagtg tttttagggt tttgaatatc atgccaacca ttgggggaag
     5401 atcatgggga aacgggttga aacctaatgt gcattcttgg tatgcagcgt gattagaggc
     5461 gtttttcttc aaacaacgat tcaatgatag gacagtcagg aaccatacct ccttcgcatt
     5521 gggaggaaat cgaacgaagt atcttttgaa gccttcgcag atctgagatt ttcctactga
     5581 catccctgag atgttgctcc gtcaccccct tcacttcccg gcacgtgtac cgcttgccgt
     5641 caaccagcct gaacagggta cgaatctcct caagcgaaaa tcccagttcc cggctgcgac
     5701 ggataaagac cgaccgtttg agttgttcct tgccatagag acgatgtccc ccttcggttc
     5761 tcggtggttt cgccagcaaa ccaacacgct tgtaatagcg gatggtctcg atgtgacacc
     5821 cggttttttg ggacaagacc ccaatggtat ccatgctccc tcgctccttg agactccatc
     5881 ttgcatctgt agttgctaca ggttgtacca tgagaatcag gtaggaagaa acctggcaga
     5941 taagtcagag aattttttca tgagccccat taactctgtg gaatgagatt ggaatcttaa
     6001 gaactatcgt gcaggaaaaa tggagatttg ccaatgggag taagcacaca acaacaatcc
     6061 gtcatacgct gtccgcaatg cggttttcaa aagaccgaaa caatgtcact tgattcctgc
     6121 caagtgagat atgagtgccg gaagtgcggg acaattctca aacccaaggc aggagattgt
     6181 tgcgtctttt gttcctatgg aacggtgtcc tgcccgcctc agcaaacaaa gcatttggac
     6241 acgtaaaccc acgcattgaa acaatcacga aggaggttgc gtataacaaa tatgaaattt
     6301 ggactgggtc tgtgcgtcgt cttcatgacc cttctcaaag gaaattgggc cgttgaggct
     6361 gagcaaattc agcctcaaca catcctgatc catatgaaga cttcttttgg ccaaagatga
     6421 tgcgaagatt tgcgccgtcc ccaacgtggc ctgggcgatg gtgaaggccg ggcatcaggt
     6481 caccgttctt gtggatgcca gtgccgtcac ctctgtcacc aaggggtttg gatggttccg
     6541 gcatctggtg cattcggaca cgaccgccct cgatggggcc aggctaccgg aaggtgaacg
     6601 gatcagtctg gctgaccgga tgggcgtgcc gttggaagag attccccatt agtatggaga
     6661 atacttggcg ttcctgaaaa agacgggtgt gaccatctat ggcaatcaaa acatgatgct
     6721 cctgaataat attgaccttg atgacgtcgc acctgaagtc acccccatcg ccttgaatcg
     6781 gatggtcgat ttttttcagt ctgcagacca caccatcgtg tattaaaaag aggtggtcag
     6841 atggacagaa aaatgcactg agaagacgcc taccggacca ctgccgccaa ggaatgaggc
     6901 tcaaaaccac aattttttta gttgcgccaa cacataggaa gggcttggcg ctgcgattac
     6961 ttggagaaca tttttgatcc gtgtgtgttc ccgcggatca agatcatgaa gtaaattcaa
     7021 taactgggtc atgtcgatga ctttccgcac ccctgcagac cgaccgttta gcgttgtgcc
     7081 caccacaaag ttctttggaa gggttcctac cgtctcagtg tcaaaggtcc cgatctgctg
     7141 agaatgttcc tcgtttctgc tgtttggatt agcccatgag gatgggacat tgcagaattc
     7201 caggacaatc caatccagca aaagcctaat taacccgcat cgcgttcggt tgaaagcggg
     7261 ctccgccgcg ttgaaatatc catcgtgcac attatttttt tcgctttttt acttccaagt
     7321 tacttcttcc caaaacaagt gaataaacgt ttcgaatgga ggaaaatttt cggtattggt
     7381 ctctgcagac cgtacccaaa gatcgatttc cattccggct ggatcagttt ctcctccggg
     7441 aaggttgcgc gtcgtcggat atggaacttg atgcgtgttg ggatctcggg aacgttccat
     7501 gaccataaaa tgggcggcat agtcctgaaa aaggagttcc ccgtttttga agacatccgc
     7561 ttttccccac cctgccaggt aaagccatgt ctttggataa agggaatcgc catttccaga
     7621 atcaccgtgt tcatagactc tcgtggccag gcctccatcc tgataaggag ccaatcccgc
     7681 aaatcgggca aagactattc ggtattgatc attgccttct acgaatttgg cttcgaccaa
     7741 accgctgttg gctgcctcat caacttcaat ttcaatcttc ccctcgatcg caaccaaatg
     7801 tttaccggcg taatccatat gatcccaggg ggctttctct cccatgctac cgaccagcat
     7861 gccatcccga ccggtcaagc gaaattgtcc actataggtg gggtgttccg tggctttgaa
     7921 gatgcgtgtc ccttcttctc ctttcggtgt accgaactga ccggctgtgg ccatacctaa
     7981 aaatataatc cctagaaata accaggtgag gcttgcgaat gtgggtttat gaagaactga
     8041 aagtatgctc atgtatcccc tccctgtagg tattcggctt ctaaaatcac tggacggaac
     8101 gcctgatggc gatcacattt tcggacgttc atgattcatc cacttcatga ctcacctcaa
     8161 agaatcaaac tttttatgat cagcccgatc aacgcgcaga cggcgatgac ctgcatgaca
     8221 ttcctccggt agcggaacag ggccacggct gctgccagcg cgatcaacgc cgatacccag
     8281 tcaaaggcgc cagtgaagcc gtgcggccat aaaacgtggt agccgaagaa cagcgccaga
     8341 ttgaggatga ccccgacaac ggcagccgtg atggcggtga gcggagccgt gaacgtgatg
     8401 tccccgtggg tcgattcgac caaaggacct cccgccagaa tgaacaggaa cgagggaaga
     8461 aaggtgaacc acgtcaccaa tgttgcggca accgccccgg cgaggaatac gaactccggc
     8521 ccgaataaga ctttcacgta cccgccgaca aacccgacaa aagccacgac catgataagt
     8581 ggcccaggcg tggtttcacc gagcgccaat ccatcaatca tttgcatggg ggtgagccag
     8641 tcgtagtatt caaccgcacc ttgatacacg tagggcagca ccgcataggc cccgccgaaa
     8701 gtcaccaggg ccgctttggt gaaaaaccag cacatttgtg tcaacgtgtg ctgccagcca
     8761 aaggtgacgg tcaggcatgc catcggtaca gcccacagca cgcttccgat gacggtgacc
     8821 ttgagcaatt tcgtccaacg aaagtaggca tgctcgggtg gcggagtgtc gtcgtcaata
     8881 agcgttaggc caaccgactt gtcggtttgg ttgtggccgc caccggctct aaacgtgtct
     8941 ggagcgatcc gaccgccgaa gtggccgatc agtccagcaa cgaggatgat cgtggggaat
     9001 ggcacattga gtgcaaagat cgccacaaag gacgccgcag cgattgccca cagcacattg
     9061 ttcttgagcg ctcgcgagcc gatcctctga gcggcttgaa ccacaatggc cgtcacggcg
     9121 ggcttgatgc cgtaaaacag gcccgcgacc agcggcacgt caccaaacgc gatatagatc
     9181 cacgacaaca cgatcagaat tagaagcgac ggcaggacga acagcgcccc ggccatgatt
     9241 ccgccccatg ttcgatgcat cagccaaccg atataggtgg cgagctgctg ggcttccgga
     9301 ccgggcagaa ccatgcaata gttcagcgca tggagaaaac gccgctcgga aatccaacgc
     9361 cgacgctcga ccagttcctg atgcatgatg gcaatctggc cggcaggccc gccgaagctg
     9421 atgaatccaa gcttaagcca gaagacaaag gcctgccaga aggtcaccgg caatggtggg
     9481 gatgcggtac tcacgactgg gtgcgcatca ggcttgttca tcggtttatt caacggctac
     9541 aaacaagcca tccaaggcgc cgcgcgcctt cgccgccagg agagtggtgc cggtgccggg
     9601 agaaggttcg ccatactcgt attcgccaca cccataaatt agattcgcat aatctgaatt
     9661 ctagcaaaac ctctatggcc ctacgaccgg attgttgcgg gcctcagtga gatttttggc
     9721 tagtcaggcg acccttctcc ctgtgaccgg ggcactcatc cgtgcatacg gtaggaaaga
     9781 acgtttcaac gaatggagaa acgggtgaag ccttattaag agcaagttga ccagctcagc
     9841 cttcctcagt cggattatgc catccaccaa tatccgccac gacggcttga gcctcattgg
     9901 gaccccacgt ccctgccgca tattcaaaga caggagtgga catcttcaaa atagggtcca
     9961 cgatgcgcca ggcctcttcg actccgtcct gcctggcaaa caaagaggca tctccttcca
    10021 tcgcatcacc gatgagacgt tcataggccc cctgttcatc gccttgttgg cgacatacgt
    10081 agagctcgat atcttcgccg atcatccgtt cgcccggcct cttcgccctg gcgcccatcg
    10141 caatcgcgac ccggtccgga cccaaccgga aacgaatatg attcgatgca tcagaccgag
    10201 tatcgtcaaa gagctgatga ggcggatgtt gcagtttgac cagcacctcc gtggccgtta
    10261 ccgggaggca cttcccagtt cgaatgaaaa aggggacgcc ttcccaccgc cacgaatcaa
    10321 tgtgaagctg catcgaggca aaggtttcga cctgcgaatc cggcctcacc ccggcctcat
    10381 cacgatagcc acggaactgt ccgcgtacca aagattttcc ggtgagtggc cgaatgccct
    10441 tgaacgcctt cacccgttca tcgcgcagag cttccccacc ggaactgaca ggtggttcca
    10501 tggccaggtt ggcaaccact tgcaacaagt gattctgcac gacgtcacgg atcgcaccgg
    10561 tttcctcata aaactttccc cgcccctcta ctccgaaccg ttccgccatc gtgatctgca
    10621 cgctttcgac gtagttacgg ttccagatgg gttcaagaaa tgaattggca aatcgaaaaa
    10681 acagaagatt tcgaatggac tcctttccga ggtaatgatc gatacggaaa atcgccgatt
    10741 catcaaagac gctgcgcagg gtatcattca gggcttgggc ggaagccaga tcgcgcccga
    10801 agggtttttc cacgacgact cgtgcgccag aggcacagcc tgactttcct aagctttcca
    10861 ccacggctgg aaacagagtc ggagggatgg ccaaatagaa gagagggtgt tgcgcggtgg
    10921 ctagggtctg acgtagacgg gtaaatgtgt cttcctcctg ataatcccca tcaacacatc
    10981 tgagcaaatc ggatagtttt gcaaaggccg tctcatcgac cccaccgccc aatttctcca
    11041 gactgtcccg ggctcgttgt cgaaggtgat ccacgtcctt accgcctctg gcgacaccga
    11101 taatcggaac atcgaccttc ccatgctgaa tcatggcctg aagcgccgga aaaattttct
    11161 tttgtgcaag gtcgcctgtc gccccgaaaa gcacaagcgc atctgaacga gccttcccca
    11221 tcattccctc ctcgaccaat ccacaaagta tatcaacgca tccgggtttt ccaggataac
    11281 ccgccagtct cctgatcctt tttggaattc aatctacatt gaattcctct cctttgtaag
    11341 gagaggcaag gtgaggtaga gacctggtac cctcgggaag ctacgatggg atcagccatt
    11401 cagtgctgtg aagaggctac ctcccctccg cccctccata caaaggaggg ggactcaagg
    11461 atgatgaact ttacaatata ctcatacgaa tgtctgaagg accagtatat ccaatgggca
    11521 tgaaccaaag aagacaagtt atgtaaaatg accgttctca ctcatccgac caaataattt
    11581 caatgggaca gaatacatct tggtgtttaa catataaccc tttggagtgc tctaccccac
    11641 cgtgaggagt tcaaggatgt gcccattcgg gtcacaaaag tacacgcccc gtcccccacc
    11701 ccgatggttg atggtcatat cctcccgcgc agtcggcccg ctcccatagg gaatgtcttc
    11761 agcctgaata cgactaaaga ttcggtcgaa atcatgttca ccgaccttga atgcata
//
LOCUS       DATIAL010000370         2708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_605156, whole genome shotgun sequence.
ACCESSION   DATIAL010000370 DATIAL010000000
VERSION     DATIAL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605156"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..621)
                     /locus_tag="VLA60_11445"
     CDS             complement(181..621)
                     /locus_tag="VLA60_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HSF10020.1"
                     /translation="MIDQLIQFAESWVPREIWVGMITLSLIAFVGTLIAIPALLIRLP
                     PDYFNNHNHRRWFANHHPIIRAIGLMVKNGLGLIFLVAGIAMLVLPGQGILTMVLGIL
                     FLDFPGKHRFEQKLIRQPQILNAINAMREKAGKLPFVFHENTPT"
     gene            767..1261
                     /locus_tag="VLA60_11450"
     CDS             767..1261
                     /locus_tag="VLA60_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HSF10021.1"
                     /translation="MMVQYWLFKSEPTTFSIDDLANSPRKTTCWEGVRNYQARNFLKS
                     MNVGDLGFFYHSNTDPPAIVGIVKVVKAAYPDAFAFDSRSRYFDPRSSPDSPRWFLVD
                     VQFVKKFPQALPLDQLRVVKGLEKMELLRKGSRLSIQPVRPDEWQRVLDLVPKNSRHS
                     STPK"
     gene            complement(1321..1608)
                     /locus_tag="VLA60_11455"
     CDS             complement(1321..1608)
                     /locus_tag="VLA60_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HSF10022.1"
                     /translation="MKFVILGKDGPEGAQKRPRYREAHLKRLEAWAQQGKVILAGPLT
                     DKIGSLIVVEADSLEEVQAFAKDDPYTLHGVFQEVTIHPFLQVFPKANLSL"
     gene            complement(1772..2188)
                     /locus_tag="VLA60_11460"
     CDS             complement(1772..2188)
                     /locus_tag="VLA60_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HSF10023.1"
                     /translation="MSDSGFPFSSERIFTLSEANGLIPELEQFWNNIKAGRQILMDTR
                     EEVKKASAQASSGGGTVMGVQYIKGLQDINSSLHAIQELGVVIKDVEIGLCDFPFLLD
                     DRLVFLCWKSGEKQVRWWHDIDSGYANRQPLEDPNP"
BASE COUNT          699 a          604 c          643 g          762 t
ORIGIN      
        1 agtccgttca ctttttcgtg aggattgact aggcctacga catggccgaa ggccacggca
       61 caaagtccgg cgagtcctag ccatccgatc ttgttccata cagacatgcg ggccatccta
      121 tcaatgggag aaaagggtgt aaacgggtgt tgactgagga aggttcaggc cgacatgtgg
      181 ttaggtcggg gtgttttcat gaaacacaaa cggcagtttg cctgcttttt ctcgcatggc
      241 gttgatagcg ttgagaatct ggggttgccg aatgagtttt tgttcgaaac ggtgttttcc
      301 cggaaaatcc agaaataaga tacccagaac cattgtgagg ataccttgtc ctggtaagac
      361 cagcatggca attccggcta ccagaaaaat gagtcccagg ccgtttttca ccatcaaacc
      421 aatggcgcga ataatgggat gatggttggc aaaccacctg cggtggttgt gattgttgaa
      481 gtaatcaggt ggcaggcgaa ttaatagagc agggatcgca ataagggttc cgacaaacgc
      541 aatcagggaa agggtgatca tgccgaccca aatttccctg ggaacccagg actcggcaaa
      601 ctgaattaat tgatcaatca tggttggtca tgatgcctca aaaatattaa gtaatcatgc
      661 ggtaaatgcg ttaggaaagg gtccaattgt actctgaaaa atgtacactt tataattact
      721 taatggtgtt ggtggttttt aaagccaatc aatgaatgag tgaggcatga tggtacagta
      781 ttggttattc aaatcagagc cgacaacatt ttccattgat gatctggcta actctccccg
      841 taagactaca tgttgggaag gcgtacgtaa ttaccaggct cgaaatttcc tgaaatccat
      901 gaatgttggg gatttgggat ttttttacca tagcaatacg gaccctccag ccatagttgg
      961 aatcgttaaa gtggttaagg ccgcttatcc tgacgccttt gcattcgatt caaggagccg
     1021 atattttgat ccgcgtagtt cgccggattc gccacgttgg tttcttgtgg atgtccagtt
     1081 cgttaaaaag tttcctcagg ccttgccttt ggatcagttg cgagtcgtta agggattaga
     1141 aaaaatggaa ctgcttcgta aaggttcccg actctcgatt caaccagtgc gtccggatga
     1201 atggcaacgg gtattggatt tagttccgaa aaattcaagg cattcgtcta ccccaaagta
     1261 ggccatcagg gttttcccaa gggttcccgt acattcttct tccacttact ttcttcccgg
     1321 ctaaagtgaa aggtttgctt tgggaaacac ctggagaaac gggtggatgg tgacttcctg
     1381 aaaaacccca tgaagggtat agggatcatc cttggcaaat gcctggactt cttcgagtga
     1441 gtctgcttca accacgatta agctccctat tttatcggtc agaggcccag ccaaaataac
     1501 tttgccttgt tgtgcccacg cctcaagccg tttgaggtgg gcttcccgat agcggggtcg
     1561 tttttgtgcg ccttcaggac cgtctttacc taaaatgacg aatttcattc tttctcctcg
     1621 ttccttttcc gacgccgcat caattggctt tcttcttcaa tgagcatgac ttctccatca
     1681 tatcagaagg cagtcatatg gacatgttgg gtcggaagcc ccggttgtct ggagagtgcg
     1741 tttgaaccga ataggcgaat gtgcggcagg actatggatt cgggtcttct aacggttgtc
     1801 tgttcgcata tccgctgtca atatcgtgcc accagcgaac ctgtttttct ccggatttcc
     1861 aacataaaaa taccaggcgg tcatcaagca aaaatgggaa atcacataag ccaatttcga
     1921 catctttgat caccaccccg agttcctgaa tggcatgcag gctgctattg atgtcttgta
     1981 accctttgat atattggacg cccatgaccg ttcctcctcc agaggacgct tgggcgctgg
     2041 cttttttgac ttcttccctc gtgtccataa gtatttggcg tcctgcctta atattgttcc
     2101 aaaattgttc cagctcgggg attaatccgt tggcttcgga gagggtgaaa atccgttcac
     2161 tggaaaaggg aaaacctgaa tcagacatgg gagaggaccc ttccttgttg actttcaatt
     2221 accgtcatga tggtttaaca taataagatt aagggtgcca accttgagag agcagggtga
     2281 ctattcgatg agtggttcgg ggaaaaattt gagtttcctg tcgcaacttc cgaaaggagc
     2341 ctattgacaa gttgttcaat agtgagataa ataattactt aagacctgcc catttgatag
     2401 caaaatccat ttccgccaat taacttctcg gacttcacgt attatttcaa atcgtagata
     2461 ggacaacacg ggtggttttg acaagtgtgg ttcttcagtt tctctaaaca ttcttcctaa
     2521 aacacacaat accggtctaa atcttttaac tttattaaag tctggtgcgg ttcccttttc
     2581 gggaatcgcc aaaattcgca gcgcacaacc cagatgaaaa tgttcgtatc ctttccaccc
     2641 ctcgtaacag gctaagccca cgagaaaacc cttccggcca atgaatggag taacctaatg
     2701 tatcttgc
//
LOCUS       DATIAL010000371         4865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_497392, whole genome shotgun sequence.
ACCESSION   DATIAL010000371 DATIAL010000000
VERSION     DATIAL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497392"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VLA60_11465"
     CDS             <1..694
                     /locus_tag="VLA60_11465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF10024.1"
                     /translation="AGEVAQSSTEAARIAQETATAARSGQEEMNQTVMGMQQISQAVV
                     QAANIIATLGRSSDQIGEIVSVIEDIADQTNLLALNAAIEAARAGEQGRGFAVVADEV
                     RKLAERTTKATKEIGDKIRQIQKDTRSAVSSMDQGTSQVGHGVALANKTGEALANIHS
                     MINATVGKIQQIANATEEQSTATRQIASDLEFMTQTTRQTTSGVFQSAKACHDLRLLA
                     GDLQKLVGSMKV"
     gene            complement(733..1020)
                     /locus_tag="VLA60_11470"
     CDS             complement(733..1020)
                     /locus_tag="VLA60_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10025.1"
                     /translation="MDRLASSQADRLLLDAKKAILQDQHRRFQDLQLEGRWDEAWKQL
                     HVTLACAADLLKDSVNMLEQVVAKKKLPSSTSTDQSPGFNDSINQNPRSTH"
     gene            1442..1969
                     /locus_tag="VLA60_11475"
     CDS             1442..1969
                     /locus_tag="VLA60_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSF10026.1"
                     /translation="MRETLDASGGTQVMQNTLTKVLPHDDFEEDRGVVLQMVSFQLGD
                     EVFAIDILVVQEIIRMLDITQVPNAPHYVEGVVNLRGKVIPIINLRVRFGLSGTEPTK
                     DTRIVVVEIAHMILGFIVDSVEEVLRLSEECIEPPPPTSRGGAEECHKGVGRVNGCLV
                     LLLDLERLFSNRSFV"
     gene            1986..2747
                     /locus_tag="VLA60_11480"
     CDS             1986..2747
                     /locus_tag="VLA60_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HSF10027.1"
                     /translation="MDILSVLGILLSLGAIVGGQHLEGGHLSSILQGTAFLIVMGGTL
                     GAVMLQFPLPVFLRALSSASMVFGNVSVDMKGIITKIVELSNISRKQGLLALEGQLKT
                     LTDPLMAKGVQLVVDGTEPHKIKEILEVEVENFEEEFGTTSKVYEAFGGYAPCVGIIG
                     AVLGLIHVMENLADPSALGGGIAVAFVATVYGVGAANLLFLPMGNKIKLKAKTLIIAK
                     IMVIEGLVSVAQGENPRMIEEKLSGFLSSAEKNKK"
     gene            2783..3523
                     /locus_tag="VLA60_11485"
     CDS             2783..3523
                     /locus_tag="VLA60_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HSF10028.1"
                     /translation="MKKKHEEHENHERWLVSYADFITLLFAFFVVMYSVSSVNEGKFK
                     VLSESLVSSFNNKKTVGELSIVNLPINKNSPIQVKAKTSSAENVRAFLSVANAITAAN
                     IPQGVQITTTERGLNIRIKDDALFQSGSARLNPQIIEFIDLIAGLVKELPNLISVEGH
                     TDNQPIRSSLYPSNWDLSTARANTLVRYLIDQHHLADYRLSSTGYAGTRPVELNDTPQ
                     GQASNRRVELIVLRDTRSDTESSHPYLP"
     gene            3853..4572
                     /locus_tag="VLA60_11490"
     CDS             3853..4572
                     /locus_tag="VLA60_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HSF10029.1"
                     /translation="MNRGIYPPLAGAITLEHRMEVLSHNIGNVNTTGFKKDKPVFATI
                     LGQTSGPSVAGIDLFPLMDALPPDRSQGTLYQTGEPLDIGLEGDGFLVAQTSDGLRYY
                     RGGKMYRNANGNLVTHTGDPLMGKKGPITLPPGEVVIGADGTLSVNNQVVDKLRLEKI
                     PDGQETAKVGNLFWTVPAQSVPDKQTTVHQGMLEQSNVNPSLDMVELIKVTREYEQMQ
                     KAIKAMDELAAQAIQAGRVQG"
     gene            4654..>4865
                     /locus_tag="VLA60_11495"
     CDS             4654..>4865
                     /locus_tag="VLA60_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HSF10030.1"
                     /translation="MIRAMHTASTGMFAQQLNIDTIANNLANVNTTGFKRSRAEFADL
                     LYQISRLPGASASNVGVFPVGMQVGLG"
BASE COUNT         1282 a         1016 c         1347 g         1220 t
ORIGIN      
        1 tgcgggagaa gtcgcgcaaa gttccacaga agcggcacgg attgctcagg aaacagctac
       61 agctgcacga agtggacagg aagaaatgaa ccaaaccgtg atggggatgc aacaaatttc
      121 tcaggcggtg gtgcaggccg ccaatattat tgccaccctc ggacggtctt cggaccaaat
      181 tggggaaatc gtgagcgtga ttgaagatat tgcggatcag accaatttat tggcgctcaa
      241 tgcggccatt gaggctgcgc gggcgggtga acaagggcga gggttcgctg tggttgccga
      301 tgaagtgcgg aaattagcag aacggacaac caaggcgact aaagaaattg gagataagat
      361 ccggcagatt cagaaagata cacgaagtgc tgtttcgtcc atggatcaag gtacaagcca
      421 ggtcggccat ggggtggcgt tggccaataa aaccggagag gccttagcaa acattcattc
      481 aatgattaac gctacggtgg gaaagattca acaaattgcc aatgctacag aagagcaatc
      541 cacagcaacg cgacagattg ccagcgattt agaatttatg acccaaacca cccgtcagac
      601 aaccagtgga gtttttcagt cggccaaagc ttgtcatgac ttgcgactgt tggccgggga
      661 tcttcagaag ttggtcggat caatgaaagt gtaactcaag gatccccatg cccggtggat
      721 ggtgaaacag tcctaatggg tcgaacgggg attctggttg atcgaatcgt tgaaccccgg
      781 tgactggtcg gttgaggtcg aggagggtaa tttcttcttg gcgacaactt gttcgagcat
      841 gttgacggag tctttgagta aatccgccgc gcaggccaat gtgacatgta attgtttcca
      901 ggcctcatcc cagcggcctt caagttgaag gtcctggaaa cgtcgatgtt ggtcttgcag
      961 aatggctttt ttggcatcaa ggagtaatcg atcggcttgt gagctggcta atctgtccat
     1021 gtaatgaatc ctttatttcg agacatgcta atgaagtaat aatgctgttc accctatcag
     1081 agcagttctt gaagcctcaa ggttctgata atggaagttg gtggaaaatg ggaagaattt
     1141 ttggagattg ctgtagattc ttacctacct ctaaacgaat ctaatggctg ttgatggaaa
     1201 acaatggaaa acaagaacgc tcaagttgga aattgcgtgg tggacggttg aatatacttg
     1261 aactgtcgga aattgcattt accgaaaagg aactttccgt agggaggaaa gtgacgccac
     1321 gggaagcggg gtcagtcaag cggctggttg cgtggatggg caggttattg aggggattgg
     1381 tgaaacaaaa ggccctggcg atgttggtta ggcatcggct ctcacagggt cggaacagga
     1441 catgagagag actcttgacg cgtctggagg gacacaagtc atgcagaata ctttgacgaa
     1501 ggtattacct catgatgatt ttgaggaaga caggggggtg gttctccaaa tggtgagttt
     1561 tcagttgggt gacgaagtct ttgccattga tatcttggtg gttcaggaaa ttattcgtat
     1621 gctggatatt acccaggttc cgaatgcccc tcattatgtg gaaggggtgg taaatctgcg
     1681 tggaaaagtc atccccatta tcaatcttcg agttcgcttt ggtctgtccg ggactgaacc
     1741 caccaaggat acgcgaattg tggtcgtgga aatcgcgcat atgattttag gatttatcgt
     1801 cgattctgtc gaagaagtct tacgcctgtc tgaagagtgt attgaacccc caccccccac
     1861 tagtcgagga ggagctgaag aatgtcataa aggggtaggg cgggtgaatg ggtgtttggt
     1921 gttgttgctg gaccttgaac ggttgtttag taatcggtcc tttgtataag gaagagtagt
     1981 ccatcatgga tattttatcg gtattgggca tccttctttc actgggtgcc attgtaggcg
     2041 gacaacattt ggagggcggg catttaagct ccattctcca agggacggcc tttttgattg
     2101 tgatgggagg tacgttaggc gcggtgatgt tgcaattccc tctcccagtt ttcctcagag
     2161 ccctttccag tgcaagcatg gtgtttggga atgtgagcgt ggatatgaag ggcattatta
     2221 ccaagattgt ggagttatca aatatcagcc ggaaacaggg actcttggcc ctggaggggc
     2281 aacttaaaac attgacggat ccgttaatgg ccaagggggt gcagctggtg gtcgatggga
     2341 cggagccgca caaaataaaa gaaatcctgg aagtggaagt tgaaaatttt gaagaagaat
     2401 ttggcacgac gagcaaggtc tatgaagcgt ttggcggata tgcgccgtgc gtggggatta
     2461 ttggcgccgt ccttggtctg attcatgtga tggaaaactt ggccgatccc tcagcattgg
     2521 gtggcgggat cgcggtcgca tttgtggcca cggtctatgg ggtgggagct gccaacctct
     2581 tgtttcttcc tatgggtaat aaaatcaagt taaaggcaaa gaccttgatc attgcgaaaa
     2641 ttatggtgat tgaaggcctg gtgtccgtgg cccaagggga gaatccaaga atgattgagg
     2701 agaagttgag tggatttctt tcctcggcgg agaaaaataa aaagtaaaat gctgacggcc
     2761 ggactgttgg gtgagggggc gtgtgaagaa aaaacacgag gagcatgaaa accacgaacg
     2821 atggctggtc tcgtatgcgg attttattac cttgctgttt gctttttttg ttgtgatgta
     2881 ttcggtctcg tctgtcaatg aagggaagtt caaggtcctc agcgaatccc tggtgagctc
     2941 ctttaacaat aaaaagacgg tgggggaact ttcaattgtg aatcttccca taaacaaaaa
     3001 tagccccatt caggtaaaag cgaaaacttc ttcagcggaa aatgtccggg cttttttaag
     3061 cgtggccaat gccattactg cagcaaacat tcctcaaggg gtgcaaatca ccacgacgga
     3121 acgtgggttg aatattcgga tcaaagacga tgcgttgttt caaagtggaa gtgccaggct
     3181 gaatccacaa atcatagaat ttatcgatct cattgccggt ctcgtcaaag agttgcccaa
     3241 tcttatatcc gttgaagggc atacggacaa tcagccgatt cgatcatctc tctacccctc
     3301 gaattgggat ctttctactg cgagggccaa taccttggtc cgatatttga tcgaccagca
     3361 tcatcttgct gattaccgat tgtcttcaac cggctatgca gggacccgtc ctgttgagtt
     3421 aaatgataca ccacagggac aagcctctaa ccgtcgagta gaactcattg tgctgaggga
     3481 tacccgttcc gacaccgaat cctcccaccc ctaccttccc tagaaattat tccacgattc
     3541 gaccacaatt tcagaacatc agttgacccg atttctgcct ttcgctaggc aggatcaggc
     3601 ctatgaggaa agtaatgccg gtaggtgtcg tgtggtgtcc gggattccaa cgcctggtag
     3661 ttttagatat gaaggattca ataggcaaag attgccattt cgagctcact ggtgccgggt
     3721 ttatggaatg aatgcttccc ctccgtagaa aaactttcca ctccggtctc tcttccggtc
     3781 aaaatccgtc tccttctctt attggcacat gcgttgcaga atttctcggg tattcacgtc
     3841 aaggagggag ccatgaaccg aggtatctat ccgcccttag ccggggcgat tacacttgaa
     3901 catcgcatgg aagtcctttc tcataatatt gggaatgtaa atacgacagg gtttaagaag
     3961 gacaagccgg tcttcgcaac catcctggga caaacctcag ggccttccgt ggccggaata
     4021 gatttgtttc ctttaatgga tgcacttccg ccggatcgat cccaaggcac cttgtatcag
     4081 accggggagc cgttagatat agggcttgag ggcgatgggt ttctggtcgc ccaaacatca
     4141 gatggattgc ggtattaccg tggtggaaaa atgtatcgga atgcgaacgg aaacttagtg
     4201 acccataccg gggatccact tatgggaaag aaagggccca ttacccttcc tcctggggaa
     4261 gttgtgattg gcgctgatgg gacactttcc gtcaataatc aggtcgtaga caagttacgg
     4321 ttggaaaaga ttcccgatgg acaagaaacg gccaaggtgg gcaatttgtt ctggaccgtt
     4381 ccagctcaga gtgtcccgga taagcagacg acggttcatc agggcatgtt ggaacaatcc
     4441 aatgtcaatc cgtccctgga tatggttgag ttgatcaaag tgaccagaga atacgaacaa
     4501 atgcaaaaag cgattaaagc catggatgaa ctagccgccc aagccataca agcaggccgg
     4561 gttcaagggt aatccaaaac aggaatgagg aatgtcccaa gaccctgctg catgaacagt
     4621 tgggaataca gataaaaagg gggagcaacc acaatgattc gagcgatgca tactgcatca
     4681 acaggcatgt ttgcccaaca actcaatatt gacacgattg cgaacaatct cgccaatgtg
     4741 aatacgacag gctttaaaag aagtcgcgca gaatttgcgg atcttcttta tcaaatttcc
     4801 cgtttgcctg gagcgagtgc ttcgaatgtt ggagtctttc ctgtggggat gcaggtcggg
     4861 ttagg
//
LOCUS       DATIAL010000372        16667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_284297, whole genome shotgun sequence.
ACCESSION   DATIAL010000372 DATIAL010000000
VERSION     DATIAL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16667
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284297"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="VLA60_11500"
     CDS             complement(<1..1795)
                     /locus_tag="VLA60_11500"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HSF10031.1"
                     /translation="MTKLLDVLFYGNPLYRKIVTVESGVEDWGAGQNEDANIPQAPAN
                     LRAIPDHTRITLTWDPVPEALYYNVYFMTTKGVQIKPNELTRPVAGQEDFIPKVGITK
                     EKANCIEGAISPYEHNDLANNFCYHYAISVVTEEGESLLSEEIMSIPSPYLPVMQIGC
                     EGVDDGEFKSPTGIAIDKDGNIYVGDTDNHSIQKFDRSGKFLARWGDEPDSTEGKFYY
                     PRGIATSPEGDVYVADSGNNRIQKFDSEGNIINAWGKFGFAWRGAEAGKFDVPWGVTT
                     DSQGNLFVSDTSNARIQKFLSDGSPVLKWGRDGGYDGAFFYPRGLAVDFVGNIYIADE
                     GNHRIQKFDARGNFLLKWGKEGNAPGQFKAPWGVACDPLGYVYVVDSGNHRVQKFDSS
                     GTYLTSWGNRGLTEGQLNFPSGIAVDKEGYVYVIDSGNHRLTKFAPTEDELNRGKQEK
                     RVASDLTAPINLAVKPGDTENILSWLEVPNAVSYNLYFSTEPNLSTDTSTKIEGVTTP
                     YIHSGLTNNTAYFYAMTSVTEDGAESRLSEEQTAIPVLIDISAPQNPDIVINHGAYMT
                     NSPDMVATISAKDVDSGVAAYFISENPMTPSG"
     gene            complement(2059..2280)
                     /locus_tag="VLA60_11505"
     CDS             complement(2059..2280)
                     /locus_tag="VLA60_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HSF10032.1"
                     /translation="MRSKGTVKWFNDRKGFGFIQVEGGQDVFVHYSALQEDGFKSLKE
                     GESVEFDLVEGAKGPQAANVTKSGIAQPS"
     gene            2604..3689
                     /locus_tag="VLA60_11510"
     CDS             2604..3689
                     /locus_tag="VLA60_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HSF10033.1"
                     /translation="MIRLLDRYIFFELLRPFLTSLTGLCFIIFTKEMLRLVDLVVSRG
                     ISFAALGSIVLHLLPSFLVLTLPIACLIASISAFNRLSFDNEVIAIRAAGVSFWRINL
                     PVVVFSGIVFLLTVYLSQWGQPWANVSLKTLALSVIEDELTLAVDSGVFNEPIPGLMV
                     YVPHSENASGGKGIFISDQRNPEQPLIIVAENFHMLKQSERQQFGIRLFKGAIHQIPN
                     DVNSFHRVTFDTYDFWLSSPFSSVKDKTKRKTYQNLTQQLEESGGTDAGALRRLVEFH
                     KDTAFPVATFVLGLLGVPVGIVSKRSGKAGGFAIGVGVVIGFYLLNVLGEFLVTTLVI
                     SPFFGAWMPNIILAWFTGFLLFQAGKQ"
     gene            3718..4806
                     /gene="lptG"
                     /locus_tag="VLA60_11515"
     CDS             3718..4806
                     /gene="lptG"
                     /locus_tag="VLA60_11515"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HSF10034.1"
                     /translation="MKKISWYVFGKFSRVFLMCLVTVLTIYLVVDFFEKLRKFLKYDA
                     DLTVIFSFFVYRIPEIAFLLAPLAALMASILTVCGLNRTREITAMRSCGLSFYQIAIP
                     FFVFGGLVSLVGLSLTAVVIPLANIKAEFVQSVLIQNKPEPLLLTPERLWLRVGRNHL
                     MRIDSVTEDGVRLNGIHQYTLDDRFSLKGILEGKWATFVDEEWIMEHAIERTFQDEER
                     MQVNGLPRQTLPLSLTPDDFQNWLAQSPEHMSLYQLHDYIQRLIEDGHSPHRFLTDYW
                     SRIAYSLVPLVMILLGISVSLRGSGVREVGIAKGLGQTLAIGFFFWAAHSVGIVLGRN
                     GAVLPIIGSWIATAMFLIIGINLFLRLK"
     regulatory      5105..5195
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5282..6160
                     /locus_tag="VLA60_11520"
     CDS             5282..6160
                     /locus_tag="VLA60_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HSF10035.1"
                     /translation="MDEDAPHFTKTAVGIKELDSSHLGSVSPEKAEDTEAQGQLDQEH
                     VTRILLEFLPTIRRMAGIMAFRNPFSLDVEDLTSAGAMGLLSALKRYDPTRDIKFRTF
                     AEYRIRGMMLDEIRSMDWVPRSVRSRRDQVRQIVEAHLQKNGVPPTAQELATLLGVPI
                     EEIEGVGGCDPRLISLDEPVGQGEDECTLRDVLPDVNYLDPFVACADAEMKAALSAAL
                     QTLSARQQEVLQSYYHAGLTMKEIGRQMGLTESGVCRVHSGAIRHLRIELKRIEDGGP
                     SAPRPRNLRKTPVVSD"
     gene            complement(6161..6730)
                     /locus_tag="VLA60_11525"
     CDS             complement(6161..6730)
                     /locus_tag="VLA60_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HSF10036.1"
                     /translation="MKRNQTLLSCILIIGIETFFICLLPVSPTPVNSADIASVLSSSS
                     PVSSLPLEIIHWQESSHSDDILAALTIYLEAGGESFAGKLAVAAVIRNRMALKYHSDG
                     TVKGTVLRAKQFEPWMGRNPEEVDFDQTNRIMQDSLLAWKVVQDGRKVVDGAVLFYNP
                     QLVRTPRWAQFNRKVAKIGGHEFFNKHTI"
     gene            7203..7727
                     /locus_tag="VLA60_11530"
     CDS             7203..7727
                     /locus_tag="VLA60_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10037.1"
                     /translation="MMICLGVGVILLGTLPLSNGWAQSDQIPVLPIQPLAHAKVYLMA
                     GDYRRAVGACQENIDRNPSVEAYVYLAYVYEAIDGYLGSLAKQEDYVKVEQLALNLTA
                     RDILDLIDPPNVMPRMAQELIHEGIRQQFDVTAAMANRLSRTRTDELWAQQTRWRELK
                     PDSWWTGAPDEWQW"
     gene            7895..8242
                     /gene="trxA"
                     /locus_tag="VLA60_11535"
     CDS             7895..8242
                     /gene="trxA"
                     /locus_tag="VLA60_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HSF10038.1"
                     /translation="MSDLVAKADATNWDGEVLKASEVVMVDFWAVWCGPCQMVAPIVD
                     ELAQEYQGKLKVMKLNTDDAPEVAGKYQIMSIPSILFFKGGQVVEKIVGARPKQQFKQ
                     VIDSLLAQSTSPA"
     gene            8268..9998
                     /gene="recN"
                     /locus_tag="VLA60_11540"
     CDS             8268..9998
                     /gene="recN"
                     /locus_tag="VLA60_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HSF10039.1"
                     /translation="MLTELRISNFALIDQLHLEFPPGFLVLTGETGAGKSLLIDALVL
                     ITGGRASAEHIRFGAEEALLEACFVIPPTHPLVSRLQGDGYLLPDQQDIIVRRMLSRS
                     GRNRNFLNGQLAPLQTIQDIGPQLVDIHGQHDQQSLLSPKTQLKLLDAFGNLENVVGS
                     YQEMHREWIEKKSALDEYVVRLRDQANRQDILQYQYDELVKMQLQSGEEEALSQEYHR
                     LKHSGRLGELSNQAFTALHEGERSVLDHLGEVTEWVQELAKIDAQGESWVPLLEAANM
                     SLREVTDNLRDYRTRIEYDPERMDLIDSRLAGLQRLKKKYGKSIEDLVELTKTLEKDL
                     AQLQNGEEQVRHLQAEVTSRYESMKALAEDLSARRKTVAKHLVKEIKQELAALKMSTM
                     EVQVNIEIANGDNRFGWAGMDRVEMLLAPNPGEPLMPLGRIASGGELSRIMLALKTVF
                     AENDQTPVVIFDEIDSGIGGEAGIVMGSRLRQLAQFHQVCCITHLPQIASQAHAQFVV
                     EKTTLEDRTLTKVHEVTGVHRETEIARMLGGGTLTPTIRKTAGEMLDRGSSPLLGLTH
                     NKPKKKPAKT"
     gene            complement(10080..10748)
                     /locus_tag="VLA60_11545"
     CDS             complement(10080..10748)
                     /locus_tag="VLA60_11545"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HSF10040.1"
                     /translation="MNRLPYRELIHGQDYWIQDQALPNALEIAQRCIANPTWTLGSPW
                     RPEPWPGMRAPGALTPDELYTVESYVTTHLGIPTLTPQSDNEAGLSGHNHIQICGGAE
                     GVARPHVDSARICDYAAVLYLHPSPPTSHCGTSFYRLHLPGEPPDGNYCPKDYESLSE
                     VPGVSQEMDPTMFKEILEAPYVFNRLVAYKSDLIHSATGYFGWDHALASKRMAVVFFW
                     KTGS"
     gene            10982..11722
                     /locus_tag="VLA60_11550"
     CDS             10982..11722
                     /locus_tag="VLA60_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HSF10041.1"
                     /translation="MLNPAVSDIDQLLTETLQVIKVEEVTRHYWEQGEFLALEHLIPT
                     QVVQEFMREVERVRPQINRNFIPGHKKGGSVSFYLLQQSAPAILAFYHHQGWIDLLSR
                     IAGVPLMVCPEEDPHSCALYFYTEAGDHIGYHYDTSYYKGDRFTVLLGLQDQSSSRLV
                     CRLHTKEQGREVKELSLLTDPGTFIFFNGDKLHHAVTPLGAGEERIVLTLQYVTNPSM
                     GLAQRWFSNMKDAVGYFGWSAMFRKPHR"
     gene            complement(11744..12523)
                     /locus_tag="VLA60_11555"
     CDS             complement(11744..12523)
                     /locus_tag="VLA60_11555"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HSF10042.1"
                     /translation="MTSAQRSNKWVGVGLTDLGLVRKLNQDAFSLDNSLQLWVLADGM
                     GGHAGGEVASQIAVKAIPDVVRTQLSTETSSYVQPEKLESLLDQALESANQRIRKAAA
                     EDESLKGMGTTIVVVAITHSPRGFQAIVAHAGDSRAYLFRQGTLSLWTKDHTLMEERL
                     ALNLITAKQVRTHPLRHVLTKALGIDPELRPTIRTYPLDPSDLILMCSDGLTKMLTDE
                     EIQTIIGQKAPHAEAICRTLVNTANRLGGEDNTTVVLIGLH"
     gene            complement(12520..13293)
                     /locus_tag="VLA60_11560"
     CDS             complement(12520..13293)
                     /locus_tag="VLA60_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HSF10043.1"
                     /translation="MLQSTFLFLPGIGESTERLWWEDGIGTWDDFLEKPTAPRISSFR
                     KAQYDEDILEAQKQWKAQNSRFFNRILKARDHWRLYPNFRSQAAFLDIETNGIPLPDG
                     EITVVGIYGKGRMTTLIQGENLSGERLQAELASYDLLVTFFGSGFDLPFLKAKYPNLT
                     LDHPHIDLCFAARRLGLRGGLKAIETEVGCYRPTSLEGLTGWDAVRLWEESQLGQADS
                     REVLIQYNEADCKNLEPLADLIYNRLAGRHGLPEYIDFL"
     gene            13431..13799
                     /locus_tag="VLA60_11565"
     CDS             13431..13799
                     /locus_tag="VLA60_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HSF10044.1"
                     /translation="MTIGGIQVIAQLKPNRTAQAVVEALPIEVPVNQWGEEFYCNMPG
                     VKDYREVATTQVKVGDVAFWGMGGMLAVFFGRTPMSLGDDPVPADRVNVIGKVIGDPK
                     VLREATGASLMRVEQLPEGT"
     gene            13799..14080
                     /locus_tag="VLA60_11570"
     CDS             13799..14080
                     /locus_tag="VLA60_11570"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSF10045.1"
                     /translation="MRLNKVRVHHMAVSVIERLQASGLLQIQGKPEVVIQKLEAAILT
                     ELQVEDRLNADVREMLKQFEREFAEGRADYQKMFTMVKQKLIKERGVIL"
     gene            14127..14414
                     /locus_tag="VLA60_11575"
     CDS             14127..14414
                     /locus_tag="VLA60_11575"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HSF10046.1"
                     /translation="MNTPILSDEKQTHLAHVILTSVTDMSEAKMIGDSAHALREVKRV
                     LAEHMKAEEELVQKVRSRLQSYARPIPEGSQEWDVLYQKTYQEELRKRNLS"
     gene            14544..14783
                     /locus_tag="VLA60_11580"
     CDS             14544..14783
                     /locus_tag="VLA60_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10047.1"
                     /translation="MNDREKTMHALGAATLSILPGIGHLYLGVKRGNIFLVCGILLLI
                     ISKFFWPTGWLIYMQFAILAGFDAFAFGRRGRGLF"
     gene            14813..15688
                     /locus_tag="VLA60_11585"
     CDS             14813..15688
                     /locus_tag="VLA60_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10048.1"
                     /translation="MKQGDRPLTRLVRLVMLDWGMRILVAVIGLNASLAQAANLSVTD
                     LLTSPGKPIQLQARLTDPEAEGEIGLPDQPLEFIVHGRTVGQANTDAHGWARLDFAPS
                     MRGNLSIIVKLVTSSKAEAIEGKGVLLSWERRRPILLIDLAVLVEGELVMDPPPPELY
                     KDPGLKLGDAHPAAASELGKLAQFYYNLVYLDLTGRGHLEHIQDWLREHQFPPGMIRI
                     LPKTSTALTELIHDLKTEGWEKVTGGIGRTADFAEVLVQNRLQTVILPLPQTKGRFPR
                     RAIVLDDWSRIRRHL"
     gene            15802..>16667
                     /locus_tag="VLA60_11590"
     CDS             15802..>16667
                     /locus_tag="VLA60_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HSF10049.1"
                     /translation="MKAARPKTRFVCQECGYQGVRWSGRCPECAQWNSLREEVDRDLS
                     TLQERSVAGGTPEAVPISSIVQTQEFRLQAGIEELNRVLGGGVVPGSVILIGGDPGIG
                     KTTLLLQTLASLGTAKQVGLYVSGEESPQQIKMRADRIGIQSPNLYVAAATSLEEIFK
                     VAEQLNPGVIVVDSIQTVFTQELTSAPGSVSQVQEVGCRLMWYAKRTHVPIFIIGHVT
                     KEGVIAGPRLLEHIVDTVLYFEGDKGQSYRILRAVKNRFGPTNEIGVFEMKDEGLTEV
                     GNPSELFLTGRIG"
BASE COUNT         4191 a         3614 c         4375 g         4487 t
ORIGIN      
        1 tcccgcttgg ggtcatgggg ttttcagaga tgaaataggc agccactccg ctatccacgt
       61 ctttagctga gatagtggcg accatatcag gactattggt catgtaagcc ccatgattta
      121 tgacgatgtc aggattttga ggtgccgaga tgtcgattaa aaccggaatg gcggtctgct
      181 cttcagatag ccggctttct gcaccatctt cggtcaccga cgtcatggca taaaaatatg
      241 ccgtgttatt cgtgagccca ctatgaatat aaggagtggt cacaccttca atctttgtag
      301 aggtatctgt ggagaggttt ggttcagtac taaaatagag gttgtaactg acagcattgg
      361 gaacttcgag ccaactaagg atgttttctg tatctccggg tttgacggca aggttgatgg
      421 gggcggtaag atcgctggca acccgttttt cttgtttgcc tcgattgagt tcatcctcag
      481 ttggggcaaa cttggttaaa cggtggttgc cgctgtctat gacgtacaca tatccctctt
      541 tgtccaccgc aatccctgac gggaaattca gttgtccttc ggttaatcca cgatttcccc
      601 acgaagtgag ataggtcccg ctagaatcaa atttttggac acggtgattt ccgctgtcga
      661 cgacatagac atatcctaaa gggtcgcagg cgactcccca tggggcttta aattggccag
      721 gggcattccc ttcctttccc cacttcagga gaaagttacc acgtgcatca aatttttgaa
      781 tccgatggtt cccctcatcg gcaatataaa tattcccgac aaagtcaacc gcgaggccgc
      841 ggggataaaa gaaggcgccg tcatatcccc cgtcacgccc ccacttaagc acaggggagc
      901 cgtcggaaag gaatttttga attctggcat tgctggtatc cgagacaaac agattgcctt
      961 gggaatcggt ggtaacaccc caggggacat caaatttccc cgcttccgcc cctctccagg
     1021 caaaaccgaa ttttccccat gcgttgataa tatttccttc ggaatcaaac ttttggatgc
     1081 gattgttccc actgtcggcc acatacacat ctccttcggg ggatgtggcg atgccccgtg
     1141 ggtagtaaaa ttttccttcg gttgaatcag gttcgtcacc ccaacgtgca agaaatttcc
     1201 ccgatcggtc aaacttctga atcgaatgat tgtctgtatc gccgacatag atatttccgt
     1261 ctttatctat agcaatgccg gttggtgatt tgaattctcc atcgtcgacg ccttcacacc
     1321 cgatttgcat cacggggagg tagggcgaag gaatggacat aatctcttcg gataatagac
     1381 tttcaccttc ttccgtgacc acagaaatgg catagtgata acaaaagtta ttggctagat
     1441 cgttatgttc ataaggagag attgctcctt caatgcaatt ggctttttct ttggttatcc
     1501 caacttttgg gatgaaatcc tcttgtcctg cgactggcct ggttagttcg ttgggcttaa
     1561 tttgaacgcc cttggtggtc atgaagtaaa cgttgtaata cagggcttcc gggacaggat
     1621 cccatgtcag ggtaattcgc gtgtgatcag ggatagcgcg taggtttgct ggtgcctgtg
     1681 gaatattggc atcctcgttt tgtcctgctc cccagtcctc cactccactt tctacggtca
     1741 caatctttcg atagagcgga tttccataaa ataaaacatc gagaagtttt gtcatgctgt
     1801 tcccctgaaa ctgatgaggc agttgtgtga aatttgtaat ttatgcaata aatacaatga
     1861 cttgcgacaa agtctaccag gagaaaaaga gggaagtcaa ggaaagtctt agcaggctat
     1921 ttacctcttg gccctctttg acaacaagtc aaaggcgagg aattccagat tattccattg
     1981 gagaaaagtt gagacctaat ggcccaaaaa attcaccctt tcgtcatcag caaataaatg
     2041 cgagagagtc accactattt aagatggttg ggcaattcct gattttgtga cgttggcagc
     2101 ctgtggaccc tttgctccct ctaccaagtc aaattctacc gattccccct cttttagaga
     2161 tttaaatccg tcttcttgaa gtgcggaata atggacaaac acatcctgtc caccttcaac
     2221 ttgaatgaag ccaaaacctt tccgatcatt aaaccatttt accgttccct tacttctcat
     2281 gatggccctc cccattagag tacttgtgga tatatattac aaaaaagttg tgcgcgctta
     2341 aaaagaaaag ccgaaaaagg agtccattcc tctctcggct tcacggcgta atattcttct
     2401 cacttcttta cccattacaa aaacgaagag gatgtagcac agtttgcaga atctgtcaat
     2461 aaatgacagg gggaaattca ttccctacta ttattcgtct ataggttaat tagaccccat
     2521 atttgattaa aaatgagcaa aggggccatg ctactcagcc atcatatttc atcactttcc
     2581 acttatttga attgtccaag accttgatca gacttctcga tcggtacatt ttttttgagt
     2641 tgcttcgccc gtttttgacc agtctgaccg gattgtgttt catcattttt accaaggaaa
     2701 tgctaaggct ggtggattta gtggtatccc gaggaattag ctttgcggcc ttggggtcta
     2761 ttgtgttgca tttgcttcct tcttttttag tcctaaccct accaattgcc tgcttgattg
     2821 cctcaatttc ggcatttaat cgcctatcgt ttgacaatga ggtcattgcc attcgagctg
     2881 cgggggtcag tttctggcga atcaaccttc ctgtggtggt cttttccggc atcgtattcc
     2941 tcttaacggt atatctttct caatggggtc agccctgggc caatgtgtca ttgaagactt
     3001 tggctctgag cgtcattgaa gatgagttga ctcttgcggt tgattcgggg gtgtttaatg
     3061 aaccgatccc cggactcatg gtgtatgtgc cacactcgga gaatgcttct ggcggtaagg
     3121 gaatttttat ttcggaccaa cgcaatccgg agcaaccctt gataattgtg gcggagaatt
     3181 ttcacatgtt gaaacagagc gaacgtcagc agtttggcat tcgattgttt aagggagcaa
     3241 ttcatcaaat ccctaacgat gtcaatagtt ttcatcgggt cacctttgat acctatgatt
     3301 tttggctatc ttcgcctttt tcctcggtga aagataagac aaaacggaaa acttaccaaa
     3361 acctcactca gcaattggag gaatcaggtg gaacggacgc tggagccctt cgtcgattag
     3421 tagagtttca taaagacacc gcatttccgg tcgccacctt tgtgctgggg ttattgggtg
     3481 tcccggtggg gattgtgtcg aagcggtccg ggaaagctgg agggtttgcg attggggttg
     3541 gcgtggttat cggattttat ttattaaatg tgttgggcga gtttctcgtg acgactttgg
     3601 tgatctctcc gtttttcggt gcgtggatgc ctaacattat cctagcctgg ttcacaggat
     3661 tcctgttatt tcaagctgga aaacaatgat ccagaattct tccaagcaat ttagatcatg
     3721 aaaaaaattt cttggtatgt gtttgggaag ttcagtcgcg tcttcctaat gtgtcttgtg
     3781 accgtgctga cgatttatct ggtcgtggat ttttttgaaa aattgagaaa gtttttgaaa
     3841 tatgatgcag acctaaccgt tattttcagc ttttttgtct atcgaattcc cgaaattgct
     3901 tttctattag ccccgctggc ggctctaatg gcgtcaattt tgaccgtttg tgggcttaat
     3961 cggactcgtg aaatcacggc gatgcgcagt tgtgggttaa gtttttatca aattgccatt
     4021 cccttttttg tctttggtgg gcttgtgagt ctcgtgggtc tgagtttgac ggccgtggtg
     4081 attcctctgg ccaatattaa agctgaattt gttcaatcgg tcttaatcca aaataaacca
     4141 gaaccccttt tacttactcc ggaacgtttg tggcttcggg tgggacgcaa tcaccttatg
     4201 cggattgatt cagtgacaga agatggggtt cggttgaatg gtattcacca gtacacgctt
     4261 gatgatcgct tcagtctcaa aggcattctt gaaggaaaat gggcaacatt tgttgatgag
     4321 gaatggataa tggaacatgc gattgagcga acatttcagg atgaagaacg tatgcaagtg
     4381 aatggtctac cacggcagac gttgcctctc tctctcacgc ctgatgattt tcagaattgg
     4441 ctggctcagt ctcccgagca tatgtccttg taccaattgc atgactatat tcaaaggtta
     4501 atagaagatg gtcatagtcc tcatcgtttt ttaactgatt attggtctcg aattgcatac
     4561 tctcttgttc ctctggtgat gatactgcta gggatctcgg tgagtctccg cggaagtggt
     4621 gtgcgtgagg ttggaatagc taaaggactt ggacaaactc tggcaattgg gttttttttc
     4681 tgggctgcgc attcggtcgg cattgtctta ggacgaaatg gagcggtatt accgatcatt
     4741 ggcagttgga ttgccacagc catgtttttg atcatcggga ttaatctttt tttaaggcta
     4801 aaatgagggt tccacgattg tcatgaagag caatggaggg aatgtgtgat tgagattttg
     4861 atcatccgtt ccttttcacc attcgtgttg gtctttgatg ctgtgcgatg tatgaacttg
     4921 tcaggtattc tcagaaacac gcaagggatt ttcttaattc cccctgactc ggatccgata
     4981 acatttccat agagatagag gttgaagagt aggggaggat ccgaagatcc aaggcagaca
     5041 gttgatcggg aaatctcaaa aaaccactta cacgaggttg taattatcga ataacgaatt
     5101 caacaggtga caagggcaaa ctatccgcga gggtagggcg caaagccaag aaaccctgag
     5161 aacgttgtta tttcagggtt cgtctggtta ccgaactgtg ttggtagatc catgcgttcg
     5221 ttcatgcgcc cattccctgg aattgggcgc ttttttttgg tcaccctgga aaggccaaaa
     5281 tatggacgaa gacgcaccac attttacaaa gaccgcagtt ggtatcaaag aactcgactc
     5341 gtcccacctc ggttcggtta gtcctgagaa agcagaagat acggaggcac agggccagct
     5401 cgatcaggaa catgttaccc ggattttatt agagtttctc ccgaccattc gtaggatggc
     5461 tggaattatg gcgttcagga atcccttttc cctggatgtc gaggatttga cgagcgcagg
     5521 ggctatgggg ctgttgtcgg cgttgaagcg ctatgatcca acacgggata ttaaatttcg
     5581 cacatttgcc gaatatcgta ttcgcggcat gatgttagat gaaattcggt cgatggattg
     5641 ggttcctcgg tctgttcgat ctcgccgaga tcaagtacga caaatagttg aggcgcatct
     5701 tcagaaaaat ggggtgcctc cgactgcaca ggaattggcc accctgttgg gcgttcccat
     5761 tgaggaaata gaaggagtgg gggggtgtga tcctcgttta attagtctgg atgagccggt
     5821 tgggcaggga gaagatgaat gcaccctccg cgatgtattg ccggatgtta attatctgga
     5881 cccatttgtg gcctgtgcgg atgcagaaat gaaagcggca ttgtcagcgg ccctccagac
     5941 attatcggca cgccagcagg aggtgcttca atcgtattat catgccggat tgacgatgaa
     6001 agaaattggt cggcaaatgg gtctgaccga gtctggcgtc tgtcgggtac attccggtgc
     6061 cattcgacat ctacgcatag agttgaaacg tattgaggat gggggaccca gtgcgcctcg
     6121 acccaggaat ttacgaaaaa cgcccgttgt cagcgactaa ttaaatggtg tgcttattga
     6181 aaaattcgtg tccgccaatt tttgccactt tacggttgaa ttgagcccac cggggagttc
     6241 tgaccagttg gggattataa aacagtaccg ccccatccac cacctttcgc ccgtcttgta
     6301 caaccttcca ggctaacaag ctatcttgca tgattcgatt tgtttgatca aaatctactt
     6361 cttctggatt acgtcccatc cagggttcaa actgtttggc tcgaagaact gtgcccttga
     6421 ccgtgccatc gctgtggtat ttgagagcca ttcggttccg aatgacggcc gcgacagcta
     6481 actttccagc aaaactttca ccaccggctt ccagatagat ggttagggcg gctaaaatgt
     6541 cgtcgctgtg gctggattcc tgccaatgga ttatttctag aggcaatgat gatacaggcg
     6601 aagaagatga aagaaccgat gcgatatcgg ccgaatttac gggagttggt gaaacgggga
     6661 gtaagcatat gaagaaggtt tctatgccga tgatgaggat acaagacaac agtgtttggt
     6721 ttcgtttcat tttatccctt taaatattga tgggtttaaa atttttacgc atttgttaga
     6781 tatggatgag tcaggttcaa taaaatgctt cttggataag gtcttccgca aagtacatgc
     6841 cattgacgtc ttagactgtt aaatggaatg tgtttatggg agtcttgtgt tgtagggagg
     6901 agtttggtgt taaacttttt gtgttttcat aaataacaag tggcctttac ccggcattgt
     6961 gtcgtggttt cgggacatca aagattaggc cacacgtttt attatttcag agtggttgga
     7021 aacagggcga tttcgagatt cttgtactga tcaaaaatct gacaatctta aaaatgggtt
     7081 aagtgaatca tcgaaaaata tggagaaagg acagagaatc tattgagaat gatgtcggtt
     7141 gcgcgatttt agtgatggag gagaacatga gcatttcgtc ggtgagcgaa agagtaaggg
     7201 aaatgatgat atgtctcgga gtgggggtaa ttcttctggg tacgttaccg ctttccaatg
     7261 gttgggctca gtcagatcag atacctgtcc tgcctattca acctcttgcg catgctaaag
     7321 tgtatttgat ggccggagat taccgtcgag cagtaggggc ttgccaagaa aatattgacc
     7381 gcaatccctc ggttgaagca tacgtgtatt tggcctatgt ctatgaggcc attgatggct
     7441 atttaggatc attagcaaag caggaggatt atgtgaaagt ggagcaatta gccctgaatt
     7501 tgaccgcacg ggatattctc gatcttattg atccgccaaa tgtcatgcct cgtatggcgc
     7561 aagaactcat tcatgaaggc attcggcaac aatttgatgt gacagcggca atggccaatc
     7621 ggttgagtcg gacacggacg gatgaattgt gggcacaaca aacccgttgg cgtgaattga
     7681 agcctgattc atggtggacc ggcgccccgg atgaatggca atggtgaatt ctatccctgc
     7741 cttgtctgtg ggtgatgagg tcaaacagat cacacagggt gcaatgctac tcagtcagtt
     7801 aatcgtatat gaatgagttg acagtccgac aagagcggtt ttaggatgcc aagacagtct
     7861 ggacatttgt cgtatttatc caaggaggca gtcagtgtct gatttagtgg ccaaagccga
     7921 tgcaacaaat tgggatgggg aagtactgaa agcttcagag gtggtgatgg tggatttctg
     7981 ggcagtttgg tgtggaccct gtcaaatggt ggccccgatt gtcgatgagc ttgcacagga
     8041 ataccaaggc aaactcaaag tcatgaagct caacaccgat gatgctccag aagttgcggg
     8101 gaaatatcaa atcatgagta tcccaagtat tctcttcttc aagggtggcc aagttgtcga
     8161 aaaaattgtt ggtgcaagac cgaagcaaca attcaaacaa gtcattgact cgctcctggc
     8221 tcaatccact tcccctgcct gatcagcggg aacgatgact cggtgttatg ctgaccgagc
     8281 tgcgtatttc caactttgcc ctcatcgatc aactccattt ggaatttccc cctgggtttc
     8341 ttgttttgac gggggagacc ggggcgggaa aatctcttct cattgatgct ctggtgttaa
     8401 tcaccggggg acgagcctct gccgagcata tacggtttgg tgccgaggaa gctctgctag
     8461 aagcctgttt tgtcatccca ccgacccatc ctcttgtgag caggttgcaa ggggacggat
     8521 acctccttcc cgaccaacag gacattatcg ttcgtcggat gttatcgcgg tccggaagga
     8581 atcgcaattt cctcaatggg caactcgccc ctcttcaaac gattcaagac atcggacctc
     8641 agttggtaga tatccatggc caacatgacc agcaatcatt gctctcccct aaaacccagc
     8701 tcaaattgct ggatgcattt gggaatcttg agaatgtcgt cggatcctat caggagatgc
     8761 accgagaatg gatcgagaaa aaatcggctt tggatgaata tgtggtcagg ttacgggatc
     8821 aagccaatag gcaggatatc cttcaatatc aatatgatga gctggtcaaa atgcagttgc
     8881 agtccggaga agaagaggct ctcagccagg aatatcatcg gttgaagcat agcggtcgat
     8941 taggcgagtt atcgaatcaa gcctttacgg cgttacatga aggcgaacga tcggttttgg
     9001 atcatctggg tgaggtgact gagtgggtac aagagcttgc aaaaattgat gcgcaaggtg
     9061 aatcgtgggt ccccttattg gaagcagcga acatgtcgtt gcgggaagtg accgataacc
     9121 ttcgggatta ccggactcgg atcgagtatg acccggagag aatggatctg attgatagtc
     9181 ggttggccgg tcttcagcgg ctgaaaaaga aatatggaaa gtcaattgaa gatttagtgg
     9241 aacttaccaa gaccctggag aaagatttag cccaactcca aaatggagaa gagcaggtgc
     9301 ggcatttgca agcagaagtg accagtcgat atgaatcaat gaaggctttg gcggaagact
     9361 tgtcggcccg gaggaaaacc gttgcgaagc atttggtcaa agagatcaag caggaattag
     9421 cggctcttaa aatgtcgaca atggaggtgc aggttaatat tgaaatagcc aacggagaca
     9481 atcgatttgg gtgggcagga atggatcggg ttgagatgct tctggctccc aatccgggag
     9541 aaccgctgat gccattgggc cggattgcat cgggtggaga attgtcccga attatgttgg
     9601 ccttaaaaac cgtgtttgca gaaaatgatc agacgcccgt cgtcattttc gatgagatcg
     9661 acagtggaat tggtggggaa gccggtatcg tcatgggaag tcgcttgcgt cagttggctc
     9721 aatttcatca agtctgttgt atcacccatc ttccgcagat tgcctcgcag gctcatgcac
     9781 agtttgtggt tgagaaaaca acgctagagg accggacgct cacaaaagtc cacgaagtca
     9841 caggtgttca tcgggaaacc gaaatcgctc gcatgttggg aggaggcacg ctgactccaa
     9901 ctatcagaaa aaccgccggt gaaatgctgg atcggggaag cagtcctctc ctgggcctca
     9961 ctcacaataa gccaaaaaaa aaacctgcca aaacctaatc taatttcgat taagcgtggt
    10021 acattcagga taggcattca tgaacgagtg gacagctctt tctccgtgtt cgagagttgt
    10081 taagatccgg ttttccaaaa aaacacgaca gccatccgtt tggaagctaa cgcatgatcc
    10141 cagccaaaat accctgttgc cgaatggatg agatccgatt tataggcaac gaggcggttg
    10201 aagacatacg gggcctctag gatttctttg aacatggtcg gatccatttc ctgtgatacc
    10261 ccaggcactt cgctaagact ttcgtaatct tttggacaat agttgccgtc tggtggttct
    10321 ccgggaagat gcaatcggta aaaggatgtg ccgcaatgtg atgtgggcgg gcttggatga
    10381 aggtagagga cggcggcata atcgcaaatt ctggcagagt cgacatgtgg acgagcaacc
    10441 ccctcagctc ctccgcatat ctgaatatga ttgtgtccgg aaagtcctgc ctcgttatcc
    10501 gattggggag tgagggtggg aatacctaaa tgtgtggtca cgtagctttc cacggtgtac
    10561 agttcatcgg gagtcagagc tccaggagcc cgcatgccgg gccagggttc cggccgccag
    10621 ggggatccca aggtccaggt tggattggca atacatcgtt gggcaatctc taatgcgttg
    10681 gggagggcct gatcctgaat ccaataatcc tggccatgaa ttaattcgcg ataggggagt
    10741 cgattcataa atacaagtta gacagagtgg ttgatcaaag gagaaatttg tgagacgaca
    10801 tgagaaagtg tggatgaaaa tgggcacctt aaaacgtcag tcagggtgag gtcaagcgga
    10861 tggattgttt gatagcctga tgagaaagtg atggttgtca tgataagcta tggttaatga
    10921 cgacctggaa aaataggata aactgaaggt ttggagtctg aagggatgaa ggagagtgac
    10981 tatgctcaat ccagcggtgt ccgacattga tcaactgttg actgagaccc tgcaggtcat
    11041 caaggttgag gaggtcacgc gtcactattg ggagcagggt gagttcttgg ctttagagca
    11101 tttgatcccc actcaggttg ttcaggaatt tatgcgggaa gtagagcggg tgcggccaca
    11161 aatcaatcga aactttatac cagggcataa aaaggggggg agtgtcagtt tttatcttct
    11221 gcagcaatcg gctccggcca ttcttgcgtt ctatcaccac cagggatgga tcgacctctt
    11281 gagccggatt gccggagttc cgttaatggt gtgccccgaa gaggatcccc attcctgcgc
    11341 actgtatttt tacaccgagg ctggagatca tatcgggtat cattatgata cctcgtatta
    11401 caagggcgat cgatttacgg ttttattagg tttacaggat caatcaagca gccgattggt
    11461 ctgccgttta cataccaaag agcagggtcg agaggttaaa gagttatccc tcctgactga
    11521 ccccggcaca tttatttttt ttaacggcga taaattgcat catgccgtta cgccgctggg
    11581 ggctggagaa gagcggattg tgctcacgct tcaatacgtc accaacccat ccatggggct
    11641 ggcccaacga tggttttcga atatgaaaga tgccgtggga tattttggat ggtcggccat
    11701 gtttcgaaaa cctcaccgtt aaaaaaaatc tccacgtccc tcctcagtgc aacccgatta
    11761 acacgacagt tgtattgtct tctccaccta atcggttggc agtattcaca agtgttcggc
    11821 atattgcttc ggcatgtggg gctttctgac cgataatagt ttgaatctcc tcatcagtca
    11881 gcatttttgt gagtccgtct gaacacatca gaatgagatc tgacggatca agcgggtagg
    11941 tcctaatggt aggtcgtaac tcaggatcaa ttccaagggc tttggttaac acatgccgaa
    12001 gggggtgagt gcgaacctgt ttggctgtaa tgaggttcag ggccaaccgt tcctccataa
    12061 gggtgtgatc ttttgtccag agcgaaagtg tgccctgcct aaagagatag gcgcggctgt
    12121 cgccggcatg tgccacgatt gcctggaatc ctctaggaga atgcgtgatg gcgaccacga
    12181 caatggttgt tcccattcct ttcagggatt cgtcttccgc tgctgctttt cgaatacgtt
    12241 gattggcgga ttccagagct tgatccaaaa gcgattctaa tttttcaggt tgaacatagg
    12301 aagaggtttc cgtcgagagc tgggttcgaa cgacatcggg aatggctttg accgcaatct
    12361 ggctggctac ctcgcctccg gcatgtccgc ccatcccatc tgccagcacc cacaattgta
    12421 gggaattatc gagtgaaaag gcatcctggt tcagttttct aaccagaccg aggtccgtca
    12481 gtccgacgcc gacccatttg ttagagcgtt gggcagatgt cataggaaat caatatattc
    12541 agggagtcca tgacgtccgg ccaggcggtt gtaaataagg tcggccaaag gctccaggtt
    12601 tttgcaatcg gcttcattat attggataag aacctctctt gagtccgctt gtccgagttg
    12661 cgattcttcc cacagccgga ccgcatccca ccccgtcagt ccttccagcg aagttggtcg
    12721 ataacaccct acttccgtct caatcgcctt cagcccgcct cttaatccga gacgtcttgc
    12781 cgcaaaacac aagtcgatat ggggatggtc aagcgtcaag tttggatatt tggccttcag
    12841 aaaaggcaag tcaaagcctg agccaaagaa ggtcacgaga agatcgtatg aggcaagctc
    12901 tgcttgcaag cgttcaccgg acaagttttc cccttgtatc aacgtggtca tgcgaccttt
    12961 tccgtaaata cccaccaccg taatctcgcc atcgggtaac ggaattccgt ttgtctcaat
    13021 atccagaaac gctgcctgag atcgaaaatt tggataaaga cgccaatggt cgcgcgcttt
    13081 caggatgcgg ttaaagaaac gggaattctg tgccttccac tgtttttgtg cttccagaat
    13141 gtcttcatca tattgggctt ttcgaaacga ggaaatgcgg ggagcggtag gtttctcgag
    13201 gaaatcgtcc catgtgccaa tgccatcctc ccaccagagg cgttcagtgg attctccaat
    13261 gccgggtaaa aaaagaaatg tggattgtaa catgagtctt aacgcgaaga gagcctaatt
    13321 ctaaccttga tttcaccaag aggacaagct acattcgaag caatcttgag atcttctttc
    13381 attcgtagca taagcgagaa tatatggaat tccgtccaca gaaaattaaa atgaccatcg
    13441 gtgggatcca ggtcattgca caactcaaac cgaatcgaac cgcacaggct gtggtcgaag
    13501 ctcttcctat tgaggtgccc gtgaatcagt ggggagaaga gttttattgc aacatgccgg
    13561 gggtcaaaga ctatcgagaa gttgccacca cacaggtaaa agttggcgat gtggcttttt
    13621 ggggcatggg ggggatgttg gcggtctttt ttggacgaac tcccatgagc ctcggagatg
    13681 atccggttcc tgctgatcgg gtgaacgtca ttggaaaagt gataggtgat ccgaaagtgt
    13741 tgagggaggc cacaggggcg tcccttatga gagtggaaca gcttcctgaa ggaacctgat
    13801 gcgactcaat aaagttcgcg tacatcatat ggccgtctca gtcattgaac gattacaggc
    13861 gagtggattg ttacaaattc aaggaaaacc ggaagtggtg attcagaaat tggaagcagc
    13921 cattctaacc gagttacagg tggaagatcg tttgaacgcg gatgtccggg agatgttgaa
    13981 acagtttgaa cgagagtttg cggaaggtcg agcggattat cagaaaatgt ttacgatggt
    14041 gaagcaaaag ctcatcaaag aacgcggggt cattttatag ggaatatggc agaatagact
    14101 ttgaaggccg taggactgaa ttgctaatga atactcccat ccttagcgac gaaaaacaga
    14161 ctcatcttgc acatgtaatt ctgaccagtg tcaccgacat gtccgaggca aagatgattg
    14221 gcgattcggc tcatgccctt cgtgaagtga agcgggtgtt ggccgagcat atgaaagcgg
    14281 aagaggagct ggtgcaaaaa gtgcggagtc gattgcaatc atatgcacgg ccgattccag
    14341 aaggttccca agagtgggat gtcctttatc aaaaaaccta ccaggaagaa ttacgtaagc
    14401 ggaacctaag ctgaagaatt cctctttgca cctctccggt ttttttcttt tctgcgagtg
    14461 tgccgtcaac acttttatcg ggcagatgct tatccctgga actctcttga gggccaatgg
    14521 tatggttttt caagggtact ctcatgaatg atcgtgaaaa gaccatgcat gctcttggag
    14581 ccgccaccct ctccatttta ccggggatcg gtcatctgta tttaggtgtc aaacgcggga
    14641 atattttcct ggtctgtggg atccttttat tgattatttc caagtttttc tggccgacag
    14701 ggtggctaat ttatatgcaa tttgccattc tggcaggctt cgatgcgttt gcatttggta
    14761 ggcgcggacg aggattattt taaccatctg ccagccacat ggagaccata gaatgaaaca
    14821 aggtgatcgt cccctgacga ggttagtgag gttggtgatg ctggattggg gtatgagaat
    14881 tttggtggcc gtgattgggc taaatgcttc actggctcag gcggcgaatt tgtcggtgac
    14941 tgatttgcta acatctcccg gaaagcccat tcaattacag gcacgcttaa ccgatcccga
    15001 agcagaaggg gagattggtt tgccggacca gcccttggaa tttattgtgc atggccggac
    15061 ggtgggccag gcgaataccg acgcgcacgg gtgggcccgg ctcgactttg ctcccagtat
    15121 gcgggggaac ttgtcaatta ttgtgaagtt ggtgacatca tccaaagccg aggccatcga
    15181 agggaaaggg gtcctcctct cctgggaacg tcgtcgtccc atattgctga ttgacttggc
    15241 ggtgttagtg gaaggggagc tggttatgga tccgcctccg cctgaactct ataaggatcc
    15301 tggcctcaaa ttaggagatg cccatccagc cgcagcctca gaattgggca agctcgcgca
    15361 attttattac aaccttgtgt atctggattt gaccgggcga gggcaccttg aacatattca
    15421 ggattggttg cgcgagcacc aattccctcc aggaatgatt agaatccttc cgaagacgtc
    15481 aacggcattg accgaactca tacacgatct gaaaacagag ggttgggaaa aagtgactgg
    15541 tggtatcggg cgcacggcag attttgcgga agtactcgtt caaaatcgcc tgcaaaccgt
    15601 gattttaccg ctaccacaga caaagggtcg gtttccacgc cgggccattg tcctcgatga
    15661 ttggtcacgt attcgacggc atctttgatg gagccaatta gcaaatagcc ggtcgacgaa
    15721 gggtccaacg accggatgtg ggtattgggg aatgcaaaat aattttctgc tcatgagcca
    15781 gaatgtaggg aagaagtctt ggtgaaggca gcacgaccca aaacacgttt tgtatgtcag
    15841 gaatgtggat atcaaggcgt tcgctggagc ggccggtgtc cggagtgtgc gcaatggaat
    15901 tccttacggg aggaggttga tcgagatctt tcaacccttc aagagcgaag tgtcgccgga
    15961 ggaacaccgg aggctgtccc catcagctct atcgtgcaga ctcaggaatt tcgtcttcag
    16021 gctggtattg aagaactgaa tcgagtgctt ggtgggggag tggttccagg atcggttatc
    16081 ttgattggag gcgaccctgg aattggtaaa acgacacttt tgctccaaac cctggcctcc
    16141 ctgggaacag ccaaacaagt cgggttgtat gtatcgggag aagaatcacc tcagcaaatt
    16201 aagatgcggg cggatcgtat aggcattcaa tctcccaacc tgtatgtggc tgctgccacc
    16261 tccctggaag aaattttcaa agtggctgag cagctgaatc ccggggtcat tgtcgtggat
    16321 tccattcaaa cggtttttac acaggaactc acatcagcgc cagggagtgt cagccaggtt
    16381 caggaggttg gctgccgctt gatgtggtat gccaagcgga cgcatgtccc tatttttatt
    16441 atcgggcatg tgacgaagga aggcgtgatt gccgggccaa ggcttctgga acatattgtc
    16501 gatacggtgt tgtattttga aggagacaaa ggccagagct atcgaatcct tcgagccgtg
    16561 aaaaaccgct tcgggccgac caatgaaatc ggggtttttg aaatgaagga tgaggggctg
    16621 accgaggttg gtaatccctc cgaactgttt ttaacgggac gaattgg
//
LOCUS       DATIAL010000373         4713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1466547, whole genome shotgun sequence.
ACCESSION   DATIAL010000373 DATIAL010000000
VERSION     DATIAL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466547"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VLA60_11595"
     CDS             complement(<1..196)
                     /locus_tag="VLA60_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF10050.1"
                     /translation="MKRNTLALRLLILSSVWVVVTLVVVGVLLILLFRSHVERRFDDF
                     LFDQLKGNIAASEISPTAGAL"
     gene            complement(186..941)
                     /locus_tag="VLA60_11600"
     CDS             complement(186..941)
                     /locus_tag="VLA60_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF10051.1"
                     /translation="MPKRWRSLRSKGSETTHHDKDRLSPVNLMRVLVVEDNPQVARQI
                     VQALQRGVYVVDVARDGEEGEFFGQTESYDAVILDLGLPKCEGLTVLQRWRQAGRTMP
                     ILILTARDTWREKVLGLRAGADDYLTKPFEVEEVVARIEALIRRAAGHATAVLTRGSV
                     TLDTSSQRVTIHDQPVELTALEFRTLSYFLHHPHAIISKTELTEHIYNQDFDLDSNVI
                     EVLINRLRKKLGASFISTHRGKGYQLTEFEHET"
     gene            complement(886..1233)
                     /locus_tag="VLA60_11605"
     CDS             complement(886..1233)
                     /locus_tag="VLA60_11605"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSF10052.1"
                     /translation="MNVDRQKISKKERMLLPWILGVSISLFGFIPPAYPDDDIPGYQQ
                     LKAEVVPVQQLFQKVQKEFEGIILELELEEEDLRWIYEVKVLTPQGNVLKIDYDAKTM
                     AVLTVKGQRDHSP"
     gene            complement(1682..2467)
                     /locus_tag="VLA60_11610"
     CDS             complement(1682..2467)
                     /locus_tag="VLA60_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10053.1"
                     /translation="MKSSTLSAWFLLFMTISNMSYATEGGTFSASPGLLEPMVFDLVL
                     PMGSHKHDYEFNTLFQYNFKDDAFRANPEFEYAFADGYGIEFELPTQDGVVKGYKLGL
                     QGTFHFLQNGSFIHGWQYIGEYHRHSVQFEDAGGEFEHDPGEFRDSGEFENDLLYLFG
                     YRMNTYWSMLNMMGLRLTDLGSHGHLEGLVNSNLTYSFSKKFHVGLEMNWESRPTRPD
                     LLLVMPQLHVRLVKHAKIQFGFGMERTEHQNFPVAAARVIFGF"
     gene            2779..3213
                     /locus_tag="VLA60_11615"
     CDS             2779..3213
                     /locus_tag="VLA60_11615"
                     /inference="COORDINATES: protein motif:HMM:NF033674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HSF10054.1"
                     /translation="MSRHILFITVGLFFFSTLTLWAQFQDPRHTGITTVSAAQEADDD
                     SWVSLTGNILRQLGEERYLFQDSTGTIIVEIDHEDWRNALVNPETTVRICGEVDRDWL
                     TKEIDVERVEVMTASSQGFSQLQRNDSDQTNPIDDGQTSGRS"
     gene            3478..3699
                     /locus_tag="VLA60_11620"
     CDS             3478..3699
                     /locus_tag="VLA60_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10055.1"
                     /translation="MNNLYIPGRSHAWYTPGQIFTWLQQQHFQVFSILYEDHWYVVEA
                     RDPDGTRVELKVTPKTRTIFTWKILEESC"
     gene            complement(4002..4634)
                     /locus_tag="VLA60_11625"
     CDS             complement(4002..4634)
                     /locus_tag="VLA60_11625"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HSF10056.1"
                     /translation="MELQRPKFWDNINMNDLARNFFEGLLILVPVVTTLYVAWLVLQT
                     IDGWLNIPIPGVGFLVTVGLITLTGRYASTVFVQKMLDVLERVLVKAPFVKILYTSLK
                     DLIAAFMGEKRRFDQPVLVSLVPGGHAEAVGFVTRTDLEFLGLLNHVAVYFPQSYNFA
                     GNILIFPKEQVHPLEAESADVMAFIVSGGVSGGQNGEGGVGLQDMISKGG"
BASE COUNT         1304 a         1218 c         1032 g         1159 t
ORIGIN      
        1 gtaacgctcc agccgtaggg gagatttcgc tggccgcaat attgcccttg agttgatcaa
       61 acaagaaatc atcaaatcgg cgttccacat gggagcgaaa gagaaggatg agcagcacac
      121 cgaccaccac aagagtcacc actacccaga cgctcgagag aatcagcagg cgcagggcaa
      181 gcgtgttacg tttcatgttc gaactctgtg agttggtatc ccttacccct gtgagtactg
      241 ataaaagagg cccccagctt ctttctcaat cgattgatta agacttcaat cacattagaa
      301 tccagatcaa agtcctggtt gtagatatgt tccgtgagtt cggttttaga tatgatggca
      361 tgaggatgat ggagaaaata cgataaggta cgaaattcca gcgcagtcaa ctccacgggt
      421 tgatcgtgga tggtcacccg ttggctactc gtgtcgaggg tgaccgaacc ccgcgtcagg
      481 actgcggtgg catggcccgc agcccgccga atgagcgctt ctatccgggc cacgacctct
      541 tcaacttcaa agggtttggt cagataatca tccgcgcccg cccgtaaacc caacactttt
      601 tcccgccagg tatcccgtgc agttaatata agaatgggca tcgtgcgtcc cgcttgccgc
      661 caacgttgta acaccgtcaa gccttcacat tttggaaggc ccagatctag aatcactgca
      721 tcgtaggatt cagtctggcc gaaaaactcc ccttcctcac catcccgggc cacatccacg
      781 acgtaaaccc ctcgctgcaa agcctgaact atctgcctgg ctacctgggg gttatcttca
      841 acaacgagca ctctcataag attaacggga gacaaacgat ccttgtcatg gtgagtggtc
      901 tcgctgccct ttgaccgtaa ggaccgccat cgttttggca tcgtagtcga ttttcagaac
      961 attgccctga ggggtcagta ccttgacctc atagatccag cgcaaatcct cctcttctaa
     1021 ttccagctca agaatgattc cctcaaactc cttctgtact ttttggaaca attgttgaac
     1081 aggaaccact tcggccttta attgttgata cccggggata tcgtcatccg gatatgccgg
     1141 tggaataaat ccgaacaggc tgatactaac ccccaagatc cacggcaaca acatcctctc
     1201 cttttttgaa attttctgac gatcaacgtt cattttctcc cacctccttt cccttttcgg
     1261 acatacggag aaaatctctc tggaacagaa tcttatcatg accaacctta cgccaagctt
     1321 aaaattctgt taagcccgaa atcaggatat aaacaggacc agacccttta tcttcacaat
     1381 acgcattctc acgcattcgg gatgtcctga acagtagccg gcaggtcgaa cgattggaca
     1441 ggcatgagtc cgtaagagcg aatttccttg aagacgaata gcggaagggc tgaatcccga
     1501 gatcctgacg atccatagat cgaatttcat ttcgtccacc gataggaagc ctaccctctc
     1561 cattcaaggt cttttcaaac tttcccattc agaccccact cctcatgcgc cctgatatgc
     1621 cttgaggcat ttcaagaaag tttcgtcaca ctgcccattc gatgagtgaa tcgacgaagg
     1681 atcagaatcc aaaaattacc cgagctgccg caactgggaa gttctgatgt tctgtccgtt
     1741 ccatcccgaa tccgaattgt atttttgcat gtttgactaa acgaacatgc agttggggca
     1801 tcactaacag taaatcaggg cgcgttggtc gagattccca attcatctct aatcccacat
     1861 gaaatttttt cgagaatgaa taagtaaggt tactattcac caatccctca agatgcccat
     1921 gagagccgag gtccgtcagt cgcaatccca tcatattcag catactccaa taggtattca
     1981 tccgatatcc aaagagatag agcaggtcat tctcgaactc tccgctatct ctaaactctc
     2041 caggatcatg ttcaaactct ccaccagcat cttcaaactg tacactatgc cgatggtatt
     2101 ctccgatgta ttgccagccg tgtataaagc ttccattttg gagaaaatga aatgtacctt
     2161 gcaatcccag tttatagcct ttgacgacac catcttgcgt cggcaattca aactcaattc
     2221 catagccatc tgcaaacgca tactcaaatt caggattggc tctaaatgca tcatccttga
     2281 aattatattg aaacaaggta ttgaactcgt aatcatgttt gtgggatccc attggtaaaa
     2341 ccaaatcaaa gaccatgggt tcgaggagac caggggaggc gctaaatgtt ccaccttcag
     2401 ttgcgtagct catattcgaa atagtcatga aaagaagaaa ccaggctgaa agcgttgaac
     2461 ttttcattcc tgattttccc tccgtccttt ctccatacat tcatcaccct gctcctatct
     2521 gaaatcgcat cctaacgatc cgacatttaa gccatgctga aatgaatttt cagcgtggct
     2581 tcaggatgcg ggaggtacaa tccccaaaga ataagcgcag cgttgaaaca ccaagaaagg
     2641 aggttcaaga aataatcgat gagatcaaca gactcatcat tcgtggtggg ttagtggtgg
     2701 gaggattcgt tgggctattg accacaacaa ggtgtaggcg gtttacacgt tcacattttt
     2761 caaccaagga gaagtatcat gagcagacac attcttttca taactgtcgg attgttcttt
     2821 ttcagtacgc tcaccctctg ggcgcaattc caggatccaa gacacactgg catcacgacc
     2881 gtgtctgccg ctcaagaggc agatgatgat tcctgggtct cactaaccgg aaacattctt
     2941 cggcaactgg gagaagaacg atatctcttc caggattcca ccggaaccat cattgtcgag
     3001 atcgatcatg aagattggcg caacgccctc gtcaatccgg aaacaaccgt cagaatttgc
     3061 ggggaagtcg accgggattg gttgacgaag gaaattgatg tggaacgggt cgaggtgatg
     3121 accgcatcca gtcagggctt tagtcagttg caacggaacg attctgatca gaccaacccg
     3181 attgacgatg ggcagacatc gggccggagc taaagtctgt tccaactatg ttgaggaaga
     3241 acggtctatc aagacttcac accctccacc aattgatgga gggtgtgagc aacagtatcg
     3301 ccggggggtt gaccctactg cattttcgag aacgggttcc gccatgccat gtgaaatgag
     3361 cagattcacc gagcagttcg tatccaaata ttgagacgtt gcgaatgaaa atcagggcgg
     3421 tatggcagaa tgaaccatca gaagatcttt ctcgatgtgc ccaaccaagg aggacatatg
     3481 aacaatttgt acattcccgg cagatcccat gcctggtata cgcccggaca aatatttacc
     3541 tggcttcaac aacagcattt tcaagttttt agcattctgt atgaggacca ttggtatgtg
     3601 gttgaggccc gtgatccgga cggcacaagg gtagaattga aggtcacacc aaaaaccaga
     3661 accatcttta cgtggaaaat cctggaagaa tcctgctgag atcgtacgca ggacggatga
     3721 gtggcttaaa gttgctgaaa gacaccgtat gcagatgaga cgacaaaagg gctccctttc
     3781 cctcgttcct aattgccttg ctttttaggt tttgcacctt cccttcagga aattgggagt
     3841 catgaataat ttcacgggcc tacttgagat agaaggtgat ttcgaaaaat cttacgtaca
     3901 aggggtaggg agattgatcg tcatgattga agcgaacgac acattcattc aaaaacattc
     3961 tccggttatc atcaattccg tcaatacaat taccatggac ctcatccacc tttcgagatc
     4021 atgtcctgta ggcctacccc cccttcccca ttttgcccac cggagacgcc ccctgacaca
     4081 ataaatgcca tgacgtccgc actttctgcc tccaatggat gaacttgttc tttgggaaag
     4141 atcaggatat ttcccgcaaa attataggat tgcgggaaat agacggccac atggttgagc
     4201 aggccgagaa attccaaatc cgttcgagtc acaaagccta ccgcttcggc atgaccaccg
     4261 ggaaccagag acaccaatac cggctgatcg aaccgccgct tttcccccat aaacgccgca
     4321 atcagatcct tgagcgaggt atagagaatt ttgacgaaag gcgctttcac caacacccgt
     4381 tccagcacat ccagcatttt ttgtacaaac acggtggacg catatcggcc ggtcaaggtg
     4441 attaacccga ccgtgaccag aaatcccaca ccagggatgg gaatattcag ccagccatca
     4501 atagtttgaa gcaccaacca ggccacatat aacgtcgtca cgaccggcac caggattaag
     4561 agaccttcaa aaaaattcct ggccagatcg ttcatattga tattgtccca aaacttgggt
     4621 cgttgtaatt ccatatcaga ctacccccat aaaaaagtga atacccgcag gcccagctct
     4681 ccagacacag acatgtcgaa acttcgcatt ttt
//
LOCUS       DATIAL010000374        12027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_68128, whole genome shotgun sequence.
ACCESSION   DATIAL010000374 DATIAL010000000
VERSION     DATIAL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68128"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..708
                     /locus_tag="VLA60_11630"
     CDS             154..708
                     /locus_tag="VLA60_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSF10057.1"
                     /translation="MNTLAIDQDAKSGTSKNNQLSPPSLKDPLYIDVRTPQEFEGMHI
                     SGARNIPLPDLHRYVSELKTLSREHRILLICRTQNRVKIAYEYLINQGLTNCGILEGG
                     ITAWVNQGKPVVRGQQRISLEGQVRAIAGVLILIGVGLGQTVHSGFLLLPTLVGVGLL
                     HAGLTDSCLMGMLLSKLPYNRIQK"
     gene            784..1305
                     /locus_tag="VLA60_11635"
     CDS             784..1305
                     /locus_tag="VLA60_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10058.1"
                     /translation="MGQGRSLLNGNARKLLLAAVIIGSATVAQAAGSSDATTQLQDIQ
                     AKFWPVLQLEGNAQKEINAIANLLSNDSKLKAIDATKASGEMCMLETVSKHNMVHFNQ
                     HPEQTTEDIVYYINPEQFIANGLDVAKLPRHPEKLGEMKPLQWYYYDGSYVEPHQGSQ
                     LNKPFVIMSLDVK"
     gene            1412..1942
                     /locus_tag="VLA60_11640"
     CDS             1412..1942
                     /locus_tag="VLA60_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1264 domain-containing protein"
                     /protein_id="HSF10059.1"
                     /translation="MQRSIRSFFMLGTFSLLAMGCMTTPAMSEGGAPAPGPAQGYDLH
                     VQAPHLMPDGTPGGPYHHYCKGINDKILQCLLFETTDANAPLVAIEYFVSKDLSRTLP
                     LIQWHRFFHDHKQEIATGRVQILDIDDPEKVKAIAEAASKTDGVIYHLWQKGKEFPDG
                     TVTYPQSLGHIFNQPN"
     gene            2009..2479
                     /locus_tag="VLA60_11645"
     CDS             2009..2479
                     /locus_tag="VLA60_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSF10060.1"
                     /translation="MDVSKRKFLIAQFLFVVGVVGIGIVHAADPEVLKPRVPPEQIQE
                     ARAKMNPFPSTSENIEKGKRVFHGKAFCVTCHGPEGKGLGDIPGLRGRLPRNFTDKTW
                     QAARTDGEIFWILQNGSPGTDMASFIPLVLTEDEAWLVILYVRSFGTGTESSGK"
     gene            2608..2949
                     /locus_tag="VLA60_11650"
     CDS             2608..2949
                     /locus_tag="VLA60_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10061.1"
                     /translation="MYKYDGKQIAEDPTKARGERPDNPPICPITDGTSTSAFVTLREH
                     RLLVVNVKNSPMTVTTTLPINEIYPAGGGCPIVPFPNDLYAIDPGGLPDSVSTKLRAS
                     RDAIFSDTYGL"
     gene            3392..3799
                     /locus_tag="VLA60_11655"
     CDS             3392..3799
                     /locus_tag="VLA60_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10062.1"
                     /translation="MTVMPLVVLATWLAFTSVETPSPSATLALPSEPDESTRIDEIYD
                     QESRLLLSLYSLKGNGKADYVTGRTVEQHVRSTYGNPVYYTLEFPIFYWWNHTMWNDP
                     FVDGVNGNEQVYLEETDFDVSRYKPCVFNGQPC"
     gene            4112..5344
                     /locus_tag="VLA60_11660"
     CDS             4112..5344
                     /locus_tag="VLA60_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF10063.1"
                     /translation="MNEKPPGIDRRHFLKGSLALGLASLLTPRGLWATDSPTVTLPFE
                     RGRRPLVAFPQKRPLMVMTTRPPQLETPFHIFNEGIFTPNDAFFVRWHLANIPREIDV
                     NTFRLTIRGRVNQSLSLTLHDLHTQFEQVEIAAVCQCSGNSRGFFHPRVPGGQWGNGA
                     MGNANWKGIRLRDLLHRAGLSGDAVQVRFDGLDQPVAQATADFRKSLTLDDALQEEVL
                     VAHSMNGEALPLLNGYPLRLVVPGWYATYWVKMLNDIEVLNQADQNFWTTHAYRLPAN
                     ACHCVKPGESLTDTVPMGKMPVRSFITNLEEGGTIPGGEPCTISGIAFDQGHGIDRVL
                     VSLDGGQQWQSARLGKDYGDFSFRPWEAKFIPKPGHAYALRSLAINRIGESQRFGARW
                     NPNGYLRNVIESVNIHAS"
     gene            5354..6019
                     /locus_tag="VLA60_11665"
     CDS             5354..6019
                     /locus_tag="VLA60_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10064.1"
                     /translation="MIRVWMFLLLLVGATGFAWGLPSDGDDREPVSILTLPADPISFQ
                     PGPGSDLASRYCLMCHSAEYVYTQPPHPREHWMEIVNKMQTTFGCPISNEDMAPLIDY
                     LVTQNSLQPSPSAQHVQRDDRKETPGQPLTQFGDPQKGKALYARHCVTCHGPGGKGDG
                     LIGQALIPPAANLTLLGKQSDQTILDTLRNGRPGTAMPAWKNDLNAVELNHLLSFLRT
                     LTP"
     gene            6236..7666
                     /gene="pyk"
                     /locus_tag="VLA60_11670"
     CDS             6236..7666
                     /gene="pyk"
                     /locus_tag="VLA60_11670"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HSF10065.1"
                     /translation="MRHVKIVATIGPATSSQECLKQLLHAGVNVARFNMSHGTADWHK
                     TTIQRLRRLALEEKIPLAILVDLAGPKIRLGDLPNEGTVLKKGQDIILIPRSDQNSRH
                     NESEDTLSVNLSHFPDRMEPGQIVLIDDGNISLTMEKQETDRLICRVLVGGQVTSHKG
                     VNFPGLRLDIPGFTEKDADDLQVAIHVQADYVALSFVRSPQDIHTLQTALTNRSSLIP
                     VIAKIERPEALTCLDEILDAADGVMVARGDLALEISPEEVPLLQKQIIAQANRMGKPV
                     ITATQMLESMTRNSSPTRAEASDVANAVLDGTDAVMLSAETSTGNFPLESVQVMDRII
                     RQTEKEMFRRIQQSESSKESVRSTPEAVCEAAVSLATSVGAQAIVVFTDSGHTAMLLA
                     RHRPTVQIIALTPSPAVTRQLILVWGIVSCVFPQIDATDERINAAQDHLKTLGLLHEG
                     RLIVIVTGERLGHSFGTNIIKAHLVV"
     gene            7691..9355
                     /locus_tag="VLA60_11675"
     CDS             7691..9355
                     /locus_tag="VLA60_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF10066.1"
                     /translation="MSLNITIVGGVAGGASAAAKARRTNETANIEMFEKGPYVSFANC
                     GLPYYVGKTIPDRDDLLLQTPERFWKRFRVRVHVLHEALKIDRLAKCVHVKNLVTQET
                     ISHPYDKLILAPGAGAIRPDLPGIHAKNIFTVKTVPDSDAIKTFLAGHPSQQALVIGG
                     GFIGLETAEALMGLGLTVTIIEKASQILPPFDQDMATLVAHHLQENGVKIIAGDGING
                     FRPDRDLAKEAELESGTRLPMDIAILSIGVRPELKLAREAGLKIGSAGGIAVNTRQQT
                     SDPDIYAAGDAVETLHLVTGKQTRIALAGPANKQGRVAGANAAGGDLQFHGALGTAIV
                     ESMGITAAKTGLSAHEAEAYGLNYFVSLTHPLDHAEYYPGAEALHMKLVVEQQTGKLL
                     GVQIVGEDGVDKRIDVLATALHARMRVQDLEQLDLAYAPQFNSAKGPVIMAGFVAANT
                     LRGEVNTLTGKELQKKLLTNPSLQLLDVRTTDEYQEAHIPHARLIPVDELRDHLQQLD
                     PAQETVVYCRVGLRGYLAARILLQHGFTHVFNLTGGYLSFPSDREI"
     gene            complement(9400..10386)
                     /locus_tag="VLA60_11680"
     CDS             complement(9400..10386)
                     /locus_tag="VLA60_11680"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HSF10067.1"
                     /translation="MDFLQNRRETAYSRLHQLKKELSKARKLVEGKACVYLIGSYGRE
                     EASKYSDLDLFIVGGGIKEEHDLNNLDEIIVKADLIEVTRKLDFPEFSGDGEYLKYYT
                     VDDLVETLGTRDDDKANTFTARLLLLLESQPLLEDEIYSKVIFKVLEAYWEDYEDHGS
                     EFVPAFLVNDILRLWRTFCVNYEAGTKNENETLEQKAKRKLKNYKLKHSRLLTCYSGL
                     LYLMAVMAEQGKVSPENAKQMVSLSPTERLEWLKGQAKIVNHSSHIDTILSLYENFLK
                     KTDAPKKELIEMFQDRIKSGQALQEAREFGDQVYELLCLIGQRNKLFRFLVV"
     gene            complement(10310..11041)
                     /locus_tag="VLA60_11685"
     CDS             complement(10310..11041)
                     /locus_tag="VLA60_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10068.1"
                     /translation="MEKISQKVRAVFDENSSRGSDRVKEQSIDVYFSADVETDGPIPG
                     PYSLLSFALVYAGRFDGHKFESPENLRQTFYREIKPISDSFELEALRVNQLDRDRLCR
                     EGKDPVDAMTEASRWVREIAGPYRPILVAYPLSFDWTWLYWYFIRFSSEGSPFNHSGC
                     FDIKTAYAVKARIPVSASGRSNLESWLRPHHEHSHHALEDAIEQAEIFANLFRWESKP
                     DGLSPKSARNRLLSPSSTQKRTVKG"
BASE COUNT         3068 a         3091 c         2860 g         3008 t
ORIGIN      
        1 catagggatt ttggaacaag ccggacgaac ccaggccatg gatcttgtcg gtgggtatga
       61 cgcctggctc accacctgga gtcatccacg ccaaaaggtt caaccccaag cgatatccgc
      121 atgcccgaaa taaacggacg aggaggatac ccaatgaata ccttggcaat agatcaagat
      181 gcaaaatcgg gaacgagtaa gaacaatcag ctgtcgcctc cttcgttgaa ggatcccctg
      241 tatatcgatg tcaggacacc acaagagttt gaaggtatgc atatttcagg tgctcgaaat
      301 attcctctac cggatttaca tcgatatgta agcgaactga aaaccctctc tcgtgagcac
      361 cgcatcctgt tgatctgccg aacacagaac cgggtgaaga ttgcctacga atatctgatt
      421 aaccaaggcc taacgaattg cggcattctg gaaggaggca tcactgcctg ggttaatcag
      481 ggcaaacctg tcgtccgggg acaacaacga atttcccttg aaggacaagt acgtgccatc
      541 gcaggggtat tgattctcat aggggttgga ttgggccaga cagttcactc agggttcctt
      601 ctccttccaa cactggtagg agttggactt ctccatgcgg gactcacgga ctcctgtctg
      661 atggggatgc tcttgagcaa gctgccctat aaccgtattc aaaaataata gttctctatg
      721 atatctactc catcattttc atgtttgaag attttacata tccaaacacc agggaggttt
      781 cagatgggtc agggaagaag tttgttgaat gggaatgcga gaaagctctt gttagcagct
      841 gtaataatcg gctcagccac agtggcccag gctgcgggga gttctgatgc cacaactcaa
      901 ttgcaggaca tccaggcgaa attttggccg gtgttgcaat tggaaggcaa tgcccaaaaa
      961 gagatcaatg cgattgccaa tttgttatcg aatgattcca agttaaaagc catcgacgcc
     1021 accaaggcca gtggggaaat gtgcatgctg gaaaccgtgt ccaagcacaa tatggtgcat
     1081 ttcaaccaac accccgaaca aacgaccgaa gatatcgtgt actacattaa ccccgaacaa
     1141 tttatcgcta acgggctgga tgtggccaaa ttgccccggc acccggagaa acttggcgaa
     1201 atgaaacccc tccaatggta ctactatgac ggcagctacg ttgaaccgca tcaggggagt
     1261 caactgaata agccgtttgt cattatgagc cttgacgtca aataatggtc gttcctagaa
     1321 ctccatggta gtatgcggcg aattttaccc ggctcccaag accttccatg tggttggagg
     1381 gtcattactg atcaacataa aggaggttgc tatgcagcgt tcaatccgtt catttttcat
     1441 gttagggact tttagtcttt tggccatggg gtgtatgacc actccagcca tgagcgaggg
     1501 gggtgcgcct gccccagggc ctgcccaagg atatgacctt catgtgcaag cccctcattt
     1561 aatgccggat ggcaccccag gtggacccta tcaccactat tgcaaaggga ttaatgacaa
     1621 gatcttgcaa tgtcttctgt ttgaaaccac ggacgccaat gcccctcttg tggccatcga
     1681 atatttcgtg tccaaggatc tttcccggac cttgccgttg atccaatggc atcggttttt
     1741 ccatgaccat aaacaagaaa ttgctaccgg ccgcgtgcaa attttagata ttgatgatcc
     1801 agaaaaagtc aaagccattg ctgaggccgc atcaaagacc gacggggtca tttatcatct
     1861 atggcaaaag gggaaggagt tcccggacgg gacagtaacc tacccacaat ctctcgggca
     1921 tatctttaat caaccaaatt aatcgaaggt ggtccccgga gattcctttc tggaatctcc
     1981 gggatctttt ctggagggga ctttgaccgt ggacgtatct aagcgtaaat ttttaattgc
     2041 gcaatttctc tttgtggtgg gggtggtcgg aataggtatc gtgcatgccg ccgatcctga
     2101 agtcctcaaa ccacgagttc ctcctgaaca gattcaagaa gcccgagcga agatgaatcc
     2161 tttcccttca acctcagaaa atattgagaa gggcaaacgc gtttttcacg gaaaagcatt
     2221 ttgcgtcacc tgtcatggac ccgaaggcaa aggcctgggt gacattcctg gccttcgagg
     2281 tagactccca cgaaacttta cggacaaaac ctggcaggca gcgcgcacgg acggggaaat
     2341 tttttggatt ttgcaaaacg gcagcccggg tacggatatg gcctcattca tccccctggt
     2401 cttgacagaa gacgaagcat ggcttgtgat attgtacgtg agatcatttg gaactggcac
     2461 cgaatcgagt gggaaatagc ataagaagaa cctttgaata attggaatca tttttggctt
     2521 tatcatgggc tggggcattt cttccggtga agcccatgaa gtgtgggtgg ccgcccaatc
     2581 cgacacggct aaagagagtg gaggattttg tataagtatg atggcaaaca gattgccgag
     2641 gaccctacca aggcccgtgg agaacggccg gataatcccc ccatttgtcc gattacggat
     2701 ggcacaagta ccagtgcttt cgtgacgtta cgtgaacaca gattactggt ggtcaacgtc
     2761 aagaattcac ccatgaccgt caccaccacg ttaccgatta atgaaattta tccggcaggt
     2821 ggcggatgtc ccatcgtgcc atttcctaat gacctctatg ctatcgatcc tgggggcctc
     2881 cctgactcgg tctcgaccaa gttgagggca tcccgcgacg caatattttc tgacacttac
     2941 gggctttgag gcattggacg caatctctgg gatgtagatc gcgcagagaa gatgattgag
     3001 atatgtcatt caatctccta ttccggcacg gggtccattc accttgtggg tacaaagagc
     3061 aatgatccgg ctccggtcct catttgattt ttttcgacac ggccattatg cttttgtgaa
     3121 cattgggggg gcccaatctc ttgaccggaa atcacaaggg tatcattaat gcgaaaggat
     3181 ccacgtctga agtggctatg accaaaatct tacatgctgg aaaaggcggt gaattgatag
     3241 gtagtacccg tctcaccaat atggtggacg gaaaagaaac agcggatccc catgggcttg
     3301 cggtagttta accctaattt gttccgtgca tgagcatggc ctccttccgg ggctatactc
     3361 tggaccacat ctaggaagga ggacttggcg tatgacggtt atgcctctag tcgttcttgc
     3421 aacctggtta gcgttcacct cagtggaaac tccctccccc tccgcgacct tagctttgcc
     3481 ttcagaaccc gacgaaagta ctcgtatcga tgaaatctac gaccaggaat ctcgcctgct
     3541 cctgtccctg tattccttga aaggaaacgg gaaagctgat tacgtcacgg gacggaccgt
     3601 cgaacagcat gtgcgaagca cctacggaaa tccggtgtac tacacgttag agttccccat
     3661 tttttattgg tggaaccata cgatgtggaa tgacccgttt gtcgatggtg tcaacggcaa
     3721 cgaacaggtc tatctggaag aaaccgattt tgatgtgagc cgatataaac cttgtgtctt
     3781 taacggacaa ccctgctaga aaaccagact tccccggcag ggtctcttcc ctcaattatc
     3841 catctggtct ctggcagatc aaggaagggc ttcgcctccc ccaaatccct catttcaaat
     3901 gaatgatctg tacgatagtc aactgtatta aatcatgaaa atgggaaccc gggaacgcac
     3961 atgatgctgt ttttcggaat ggctgatgac cggatacggt aagtgagata gtcaattcgt
     4021 gtcttggcaa aacgaatgaa aaacgtgaag gactgatttg tgcgagggcg ggctttttgg
     4081 tttctttggg agaatcacgg tagattgact tatgaatgaa aaaccccctg gtatagaccg
     4141 tcgacatttt ttgaagggga gcctggctct tgggctggca agtctactca cccctagagg
     4201 cctctgggcc accgattccc cgacggtcac tcttcctttc gaacggggtc gccgtccact
     4261 ggtggctttt ccacagaagc ggcccctcat ggtgatgacc acaaggcctc ctcaattgga
     4321 aacccccttt catattttta atgaaggcat cttcacgccc aatgatgcat tttttgttcg
     4381 ctggcatctc gccaatatcc ctcgggaaat tgacgtgaac actttccggc tgactattcg
     4441 cggtcgagtc aatcaatccc tatcattgac tcttcatgat ttacacactc aatttgaaca
     4501 agtcgaaatt gccgccgtct gccaatgctc gggaaacagt cgcgggtttt ttcatcctcg
     4561 ggtgcccggg ggacaatggg gcaatggagc aatgggaaat gccaactgga agggaatcag
     4621 actccgtgat ctccttcatc gggcaggcct atcaggggat gcggtgcaag tgaggttcga
     4681 tggcctggac caacccgtcg cacaagccac agcggatttt cgaaaatctc tcacccttga
     4741 tgatgcgctc caggaagagg tgttggtcgc tcactccatg aatggggagg ccctccccct
     4801 gctgaacggc tatccccttc gcctcgtggt gcctggctgg tatgccactt attgggtgaa
     4861 gatgctgaat gatattgaag tgctcaatca ggccgatcaa aatttttgga cgacacatgc
     4921 ctatcgcttg ccggctaatg cttgtcattg cgtaaaaccc ggagagtcct taacggatac
     4981 cgtgcccatg ggcaaaatgc ccgttcgttc ctttatcacc aatcttgagg agggtggaac
     5041 cataccggga ggggaacctt gcaccatttc aggtatagcc tttgatcagg gccatggcat
     5101 tgatcgcgtc ctggtctcct tggatggtgg acaacaatgg caatccgccc gattaggaaa
     5161 ggattatggt gatttcagct tccgcccctg ggaagccaaa tttattccga agcccgggca
     5221 tgcgtatgca ctgcgatcac tggccatcaa tcggattgga gaatcacagc gattcggtgc
     5281 ccgatggaat ccaaacggat atcttcgtaa tgtgatcgaa agtgtgaata ttcatgcatc
     5341 ctgaggccaa ctcatgatcc gtgtttggat gttcttgttg ttgttggttg gggccacggg
     5401 atttgcctgg ggacttccgt ctgatggaga tgacagagaa ccggtatcca ttctcactct
     5461 tcctgctgat ccgatttcct ttcaacctgg tccaggaagt gacctcgcaa gccgttactg
     5521 cctaatgtgc cattcggcag aatacgtgta tacgcaacca cctcatccgc gtgagcattg
     5581 gatggaaatc gtcaacaaaa tgcaaacaac ctttggctgt ccaatctcga atgaggacat
     5641 ggctccattg atcgactact tggtgacgca aaattcactc cagccttcac cctctgcaca
     5701 gcatgtccaa cgggacgacc ggaaggaaac acccggtcaa cccctcactc aatttggcga
     5761 tccccaaaaa gggaaagccc tctatgcacg acactgcgtg acgtgtcatg gacccggcgg
     5821 gaaaggggat ggtctcattg gacaggctct gattccccca gcggccaacc tcacattact
     5881 cggcaaacaa tcagatcaaa ccattcttga tacgcttcgc aacggccgac cgggcacagc
     5941 catgcctgcc tggaagaacg atctgaatgc agtagagttg aatcatcttc tttcatttct
     6001 tcgaacgtta actccataac cgcgatgaat cccgggagcc tggctattag accattccat
     6061 cagcggggga atatctttct tgggtttgcc caattacaca aatacttgcc atgcaatgct
     6121 tcatgaaacc tgagaaaaaa cctcgtgaaa tttttggttg ttgcggtcac aaaaggtccc
     6181 gcttccccca ccaatatctg ctggcccccc tttaaccaat cttgagatca ttcccatgcg
     6241 tcatgtcaaa attgtagcca caattggccc tgcgacttct tcccaggaat gcttgaaaca
     6301 actcctccat gccggagtca atgtcgccag gtttaacatg tcccatggaa ccgctgactg
     6361 gcacaagaca acaattcaac gccttcgacg ccttgctctt gaggaaaaga taccccttgc
     6421 catccttgta gacttggcag gtccgaagat ccgcctgggg gatctcccaa acgaaggaac
     6481 cgtcttgaag aaagggcagg acatcattct gattccgagg tcggatcaaa actcaaggca
     6541 caacgaatcg gaggataccc tgtcggttaa tctttcccat ttcccggatc gaatggaacc
     6601 aggtcaaatc gttttaattg atgacggaaa tatatccctc accatggaaa aacaagaaac
     6661 cgaccgactg atctgcagag tcctggtcgg aggccaggtc acctcacata aaggtgtcaa
     6721 tttccctggc ctccgactcg acattccggg atttacggag aaagacgccg atgatcttca
     6781 agtggccatc cacgttcaag ccgattatgt cgccctctct tttgttcgaa gtccgcagga
     6841 tattcataca ctccaaacgg ctttgactaa tcgatcatcg ttgattcctg tcattgccaa
     6901 aatcgaaaga cctgaagccc tgacatgtct tgatgaaatc cttgatgcgg cagatggagt
     6961 gatggtcgcc agaggtgacc tggcgttgga gataagcccg gaagaagtcc ccctcctgca
     7021 aaagcagatc atcgcccagg ccaatcgcat gggaaaacct gtgatcacag ccacacaaat
     7081 gctcgaatcg atgactcgaa attcttcccc cactcgcgca gaagcatcgg atgtggcgaa
     7141 cgccgtactc gacgggactg atgcggtcat gttgtcggcc gaaacgtcca cgggaaattt
     7201 tcctctcgaa tcagtccagg tcatggatcg gattatccga caaacagaaa aggaaatgtt
     7261 ccgccgaatc cagcaatccg agtcgtctaa ggaatccgtg cgttcgactc cggaagcggt
     7321 ttgtgaagct gccgtctctc ttgcaacatc tgtcggcgcc caagccatcg tggtcttcac
     7381 cgattccggc catacagcca tgttattggc ccgccatcga ccgacggtcc agattatcgc
     7441 cctgactccc tccccagccg ttacccgtca attaatttta gtatggggca ttgtctcatg
     7501 tgtctttccg caaattgatg ccaccgatga acgaatcaat gcggcccagg atcatctcaa
     7561 aaccctcggc ctgcttcatg aaggccgtct cattgtcatc gtcaccgggg aacgcctggg
     7621 acattccttc ggaaccaata tcatcaaagc ccatttggtc gtgtaaagtc agccaaggag
     7681 accaggaaac atgtccctca acatcaccat tgtcggcggc gtggccggcg gggcgagcgc
     7741 cgccgctaag gcgcgtcgaa ccaacgaaac ggcaaacatt gaaatgtttg aaaaagggcc
     7801 ctatgtatcc tttgccaact gtggcctccc gtactatgtg ggaaagacca ttccggatcg
     7861 tgatgacctt ctgcttcaaa ccccggagcg gttctggaaa cgattcaggg tccgggttca
     7921 tgtcctgcat gaagccctca agatagaccg acttgcaaag tgcgtccacg tcaaaaatct
     7981 ggtcactcaa gaaaccatct cccacccgta cgacaaattg attttggctc ccggagcggg
     8041 ggcgatcagg cctgacctcc ccggtattca tgccaaaaac atctttacgg tcaaaaccgt
     8101 tccggattct gatgccatta aaacctttct cgctggccac ccttcccagc aggccttggt
     8161 tattggagga gggtttattg gactggaaac ggccgaagcc ctgatgggtc ttggattgac
     8221 cgtaacgatc atagaaaaag cttcacaaat tctgcctcct tttgatcagg acatggccac
     8281 gctggtcgct catcatctac aagaaaatgg ggtgaagatt attgcgggtg acgggatcaa
     8341 cggatttcgg ccggacaggg atctcgccaa agaagccgaa ttggaaagtg gaacacggct
     8401 gccgatggac atcgcgattc tttcgatcgg cgtcagaccg gaactcaagc ttgcccgaga
     8461 ggcggggctt aaaattgggt cagcgggagg cattgccgtg aatacacgac agcaaacctc
     8521 tgaccccgat atctatgcag cgggtgatgc agttgaaact cttcaccttg tgactggcaa
     8581 acaaacaaga atcgccttag ccgggccggc caataagcag gggagggtcg ccggagccaa
     8641 tgctgcgggc ggagacttac agtttcacgg tgcactggga acggcaattg tcgagagcat
     8701 gggtatcacg gctgccaaaa cgggattaag cgcacacgaa gccgaggcct atgggttgaa
     8761 ttactttgtc tccctcaccc accccttgga tcacgcagag tattatcctg gagcagaagc
     8821 gctgcacatg aaattggtcg tcgagcaaca gacggggaag ttgttaggtg tccagattgt
     8881 gggggaggat ggcgtagaca aacgaattga tgtgttggcc actgccctac acgccagaat
     8941 gcgagtccag gaccttgaac aacttgatct cgcctatgcc ccacaattta attctgccaa
     9001 aggaccggtc attatggcag gtttcgtcgc cgccaatacg ctacgcggtg aggtcaacac
     9061 gctcacgggg aaagaattac aaaagaaatt gctgacgaac ccttctctcc agttattaga
     9121 tgtccggacc acggacgaat atcaagaagc tcacattcct catgcccgcc tcattcccgt
     9181 ggatgaactg agagaccatc ttcagcaact ggatcccgct caggaaaccg tcgtctattg
     9241 tcgtgtcgga ttacgcggat atctcgcggc acgcattctc ctccaacatg gcttcaccca
     9301 tgtcttcaac ctcaccggtg gctatttaag ctttccttcg gacagagaaa tttaaatcct
     9361 aagtagacca aatgccacga accttgaggt tttcgattgc tagacaacga ggaatcgaaa
     9421 aagcttgttt ctctgaccta ttaaacaaag cagttcatat acctgatcgc caaattccct
     9481 ggcctcttga agtgcctggc cactttttat ccggtcctgg aacatttcga ttaattcctt
     9541 cttaggagca tctgttttct tcaaaaaatt ttcatacagg gaaagaattg tatctatatg
     9601 acttgaatga ttaacaatct tggcttgtcc ttttaaccat tcaagacgtt cagtaggaga
     9661 aagggaaacc atttgcttcg cattttctgg gctaaccttg ccttgttcag ccataactgc
     9721 catgaggtac agaagtccag aatagcaggt taaaagcctg ctgtgcttaa gcttgtaatt
     9781 cttgagcttc cgtttagctt tctgttctaa tgtttcattt tcatttttgg ttcccgcctc
     9841 gtaattcaca caaaaggttc gccacaagcg gagaatatca tttacaagaa acgcagggac
     9901 aaactcagat ccatgatcct cgtagtcctc ccagtaggcc tcaagaactt taaaaatcac
     9961 ttttgagtag atttcatctt ctaaaagtgg ctggctctcc agcagcagaa gcagccgggc
    10021 tgtgaaagtg ttggccttat cgtcatcacg tgtgcctaga gtttcgacca ggtcatccac
    10081 agtataatac ttgagatact caccgtcgcc ggagaattct ggaaaatcta gttttctagt
    10141 aacttctatt aaatcggctt ttactataat ttcatcaaga ttattcagat catgttcttc
    10201 tttaattcca cccccaacga taaacagatc tagatcacta tatttacttg cctcttcacg
    10261 accgtaggac cctattagat agacgcaggc cttcccttcg acaagctttc tagcctttga
    10321 cagttctttt ttgagttgat gaaggcgaga gtaagcggtt tcgcgccgat tttggagaaa
    10381 gtccatcagg tttgctctcc cacctgaaca agtttgcaaa aatctctgcc tgctcaatcg
    10441 catcttctaa cgcatggtgg gagtgttcat ggtggggccg aagccagctt tcaagattcg
    10501 accggccgga cgctgacacc ggaatcctgg ccttgactgc ataggcggtt tttatgtcaa
    10561 aacaacccga atggttgaag ggcgaacctt cagaagaaaa gcggataaaa taccaatata
    10621 accatgtcca atcgaaactt aatgggtagg caacaagtat tggtctgtag ggacccgcaa
    10681 tttctcgaac ccatcgacta gcctcagtca tggcatctac ggggtccttc ccttcgcgac
    10741 aaagacgatc acgatcaagc tgatttacac gtaacgcctc caactcgaag gaatcagaaa
    10801 ttggctttat ctcacgatag aaggtttgcc tgaggttttc aggtgattca aatttatgcc
    10861 catcaaaacg accagcatag accagggcga acgaaagaag agaataggga ccagggatgg
    10921 gaccatccgt ctcaacatca gcagaaaaat agacgtcaat gctttgttcc ttgacccgat
    10981 cggatcctcg agatgaattt tcatcgaaaa cagcacgcac tttttgagat attttctcca
    11041 taagggccga tcctaacagt ctgaatttag ggagtccagc caataaaact aatcatgaaa
    11101 cccgactacc cattctggcc gaaccgcata tgtttcacga tcatcgccca cctttctgtc
    11161 ctccctcccg agtaactccc tatccacgcg gagtagcggt ctagacgtaa aagtctgccg
    11221 atgcacaaat ctcttctcct tcatgagctt ccttagcccg cctttatacg acgttctccc
    11281 taaactcctt aaatcaggag attccgaatt caaccgctca cacctcgaca cacatcaccg
    11341 cctcattccc gtggatgaac tgagagacca tcttcagcaa cttgacccct cgcaggaaac
    11401 ggtcgtctat tgccgggtcg gggtatgcgg ctaccttgcc gcccgcattc tgctccagca
    11461 cggcttcacc aatgtcttca acctcactgg tggctattta agttttcctg gaactgatag
    11521 ataagcttgc cggatttatc cctgggatag cgttgtttcc tcaagtgtta accacttaac
    11581 tgttaagact taacatgtga aagatttctc tcaagccttt cttcatctcc ctcccttctc
    11641 atctcgaagc tgctctaatt ccggtatttt ttcttttttt gtttgtgctc aatatggtgc
    11701 tgattttgct attcttcctt gtcagagttc ggataatgct ttgtttgggg caaaacacct
    11761 cagatgtgtg cctgcggaag aggggaattt ttcctcacat gtaaaaacaa gtggtaatcc
    11821 tggaaaaatt cggctgttaa ccaatcaacc aaatataaga atttcatctc cttaatttca
    11881 cccgtggagg ttttcacaat gaaatgcttt gcatgcctcg tctcccaaaa aggtttattg
    11941 acccttctgg tctttattct cggtggtttc atgacccttg gcttcccaac gaccattttg
    12001 gcggccgatg tacaagaggc ggagtcg
//
LOCUS       DATIAL010000375         5458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_605811, whole genome shotgun sequence.
ACCESSION   DATIAL010000375 DATIAL010000000
VERSION     DATIAL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605811"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1534)
                     /locus_tag="VLA60_11690"
     CDS             complement(116..1534)
                     /locus_tag="VLA60_11690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HSF10069.1"
                     /translation="MKTTSIRVRLTLWYGSALALILVLFAVALYLVMSRALREQVDAS
                     LEEAAAVAIRTLGEHRFGPFLIFEDLSQDFPEIALLDKFFQIFGPAGQVTIQSANIQS
                     REIPLSQTSFRASLDGKSTFESVQFEKGVPLRLLSVPIRQDDQLVNILRVGTSLYPTE
                     RMLHRLLAGLYIASPLALLVSLVGGWFLAGRALRPVHAITQAAQRIAAGDWTQRIQTP
                     HSNDEIGQLASTFNDMIGRLEVSFRQIRQFSADASHELRTPLTITKGETELALRRPRQ
                     TEDYRVVLESNLEEIDRMSRIVDELLFLSRADLGEIKLKMFPVQLDDLVREIQQQALV
                     LGQERNIHTVLEAIEPVVVQGDDLRLRELLLNLVDNAVKYSQEGQTVELSLLVAGNKG
                     KLVVRDHGIGLAPEDHGRIFDRFYRTDEARAHAAKGTGLGLAICKWIVEVHHGTIEVQ
                     SVVHGGSCFTVFLPLAPSAAKV"
     gene            complement(1531..2205)
                     /locus_tag="VLA60_11695"
     CDS             complement(1531..2205)
                     /locus_tag="VLA60_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF10070.1"
                     /translation="MRILVVEDEPKVASFIRRALEEESYAVDVCADGPGGLDWAQAVN
                     YDLIVLDLMLPGLPGLEVLKRARAAGVKAPVLILTARSEVDQRVKGLDAGADDYLTKP
                     FAIEELLARARALLRRAGGAPTGILQVDDVILNPVTREVTRAGQRLEFTTKEYALLEY
                     LMRNAGRVLTRSMITEHVWDLDFDTFTNVIDVYISYLRNKIDKGRERSLIQTVRGSGY
                     MMRSDG"
     gene            complement(2339..4162)
                     /gene="rpoD"
                     /locus_tag="VLA60_11700"
     CDS             complement(2339..4162)
                     /gene="rpoD"
                     /locus_tag="VLA60_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HSF10071.1"
                     /translation="MGKTVSLPEVIQRLIEIGKPKGHVTIKDLTHILPADQITSDQLH
                     TILGELHEVNLQVIDPSKRTASQLAALKKNAGQEDSDDSDEETDSSLDIDLTPGEPTR
                     IDDPVRLYLKEMGRVPLLTREGEIELAKHIEEGKRELACAVYGMPLNIHYLESLHDQL
                     KLEEIRVRDIVLLQETLEEEEVEEETMASEQEDEELRFRTLESLATVRKLGRGLLALY
                     TQNRDGQTTKNTKAQLEKRRIGLADQFVDQIEALNLHQRVKDQMLRRVKDVGQEIRSI
                     EMMTARHTKKLGLAGQDGIQVIGKKPRVTTKGSSLRRKQHLTPEEIEKLKQEVEAGKA
                     TLLRLQDEVLGMPMEEFTAALTILETAEEKVKRGKAQLIEANLRLVVSIARKYTNRGL
                     QFIDLIQEGNIGLMRAVDKFEYQRGYKFSTYATWWIRQGVTRAIADQARTIRIPVHMI
                     EANTKLARTARQLVQQLGREPTPEEIAERMGLTPEKVRMMLDISKGTISLETPIGEKE
                     DSQLGDLIEDKNAVSPMDAANRYDLQRQIANALGVLTPREETVIRKRFGIGDSTDHTL
                     EEIGQDFDVTRERIRQIEAKALKKLRHPSCSHKLRSLVDHL"
     gene            4786..>5458
                     /locus_tag="VLA60_11705"
     CDS             4786..>5458
                     /locus_tag="VLA60_11705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSF10072.1"
                     /translation="MISLLLVEDNPKEVLVIKNMLKEGLQNQFTLKHSRSLSDALDLI
                     QQNQFQAIILDSHLPDGKSFESIPQFLQFCPDAPILILSGVEEEDQAIQAVKSGVQDY
                     LIKGQTSSSTLCRAIRYAMERQRATRRITQLAHYDHLTGLANRGLFYERLNCAVARCH
                     RNDTAIALMFLDLDHFKDINDSLGHDCGDSLLKTVAARIKKCIREIDTGVRLGGDEFA
                     VLLEQI"
BASE COUNT         1491 a         1422 c         1122 g         1423 t
ORIGIN      
        1 tcaagagtcg cctcccttaa gatttgttca gtacaaggga aaggtaccac ccagagatga
       61 aggggaaatt aggggaaaat taaaactttt taatagagcg agaagtgttt gtgtcttaca
      121 ctttcgctgc ggagggagcc aaaggcagga aaaccgtgaa acaggaacca ccatgaacga
      181 cactttggac ctcaatggtc ccatggtgaa cctccacaat ccacttacaa atcgccaaac
      241 ccaatcctgt cccttttgcc gcatgagcac gcgcttcatc cgtccggtag aaccgatcaa
      301 aaattctgcc atgatcctca ggggcaaggc caataccgtg gtctcggacc accagctttc
      361 ccttgtttcc ggcaacaagc aatgagagct ccaccgtctg tccttcttgc gaatatttca
      421 cggcattatc gaccagattt aacagtaatt cacgcaaacg cagatcgtcc ccctggacaa
      481 cgacaggctc aatggcttcg agcaccgtgt gaatattccg ttcttgaccc aataccaagg
      541 cctgttgttg gatttctcgc actaaatcgt ccaactgaac tgggaacatc ttgagcttaa
      601 tttcgccaag atcggctcgt gataaaaaca agagctcatc cacaatccgg ctcatgcggt
      661 cgatctcctc caaattactc tccagcacca cccgataatc ttccgtttgc cgcggtcgcc
      721 gtaacgccag ctcagtctcc ccttttgtaa tggttaaggg tgtccgaagt tcatgggacg
      781 catccgcgct aaactgccgg atttgccgga acgacacttc caaccgtcca atcatgtcat
      841 taaaggtcga ggctaattgt ccgatttcat cgttggaatg tggagtttga attcgttggg
      901 tccaatcccc ggccgcgata cgttgggccg cctgcgtaat agcatgaacc ggacgcaacg
      961 cccgtcctgc caaaaaccac cctccgacca aagacaccag caaggccaaa ggagaggcga
     1021 tatagagacc ggctaagagc cgatgcaaca ttcgttctgt aggataaagg gatgtcccaa
     1081 cacgtaaaat attcaccaat tgatcatctt gacgaatagg aaccgacagc aatcgcagtg
     1141 gaaccccttt ttcaaattgg acggattcaa aggttgattt tccatcaaga gaggcacgga
     1201 acgatgtctg actcaatggg atttctcggc tttggatatt agccgactga atcgtcactt
     1261 gccccgccgg cccaaaaatt tgaaaaaatt tatccaaaag cgcaatttca ggaaagtcct
     1321 gagataaatc ttcaaaaatc aggaacgggc caaaccgatg ttctcccaac gttcgaatcg
     1381 ccacagcggc agcctcttcc aacgaggcat ccacctgttc ccgaagtgca cgggacatca
     1441 ccaaatataa agccacggca aaaagaacca gaattagagc taatgcagat ccataccaca
     1501 aagttaaccg aactcgaatc gaggtcgttt tcacccgtcc gacctcatca tatatccgct
     1561 cccccgaacc gtttgaatta aacttcgttc tcgtccctta tcgattttgt ttcgtaagta
     1621 gctaatatac acatcaatca cattagtaaa cgtatcaaaa tccaagtccc acacatgttc
     1681 cgtaatcatg gaccgcgtta acacccgccc cgcatttcgc attaaatact ctaagagcgc
     1741 atattcttta gttgtgaact caagacgttg gccggcacga gtcacctccc gagtaacagg
     1801 atttaatatc acatcatcca cctgaagaat tccggttggt gcccctccag ctcgacgaag
     1861 gagggctcgg gctctggcga gcaattcttc gatcgcaaag ggtttcgtta aataatcgtc
     1921 cgcccccgca tctagcccct taacccgttg gtccacttct gaacgtgccg ttaaaataag
     1981 taccggagct ttgacacctg cagcccgagc gcgtttaagc acttctaatc caggcaatcc
     2041 cggtaacatt aaatccaaaa caattaagtc ataatttacg gcttgagccc aatccaaccc
     2101 tcctggtcca tctgcgcata catcgaccgc ataactttcc tcctccaggg ctcgccggat
     2161 aaaggaagcc acctttggtt catcttccac gactaaaatt cgcatcttaa gaaaagcata
     2221 ccatctaaat acccggaccg agaaggggaa cacaaattga gtaaatagga aatatatggc
     2281 aaaaaaaagg ccccgtcatc tagatgacgg ggccttatcc atttacaata aactgggatt
     2341 agagatggtc caccaaactc cgaagtttgt gactgcagct cgggtgacga agtttcttta
     2401 gggctttggc ctcgatctgg cgaatccgtt cacgagtcac gtcaaaatcc tggccaattt
     2461 cctccaatgt atggtcggta ctatcaccga tcccaaaccg cttacggatc acggtctctt
     2521 cgcgaggggt cagcacgccc aaggcattag caatctgacg ttgcagatca taccgatttg
     2581 ccgcatccat gggagagact gcatttttat cttcaatcaa gtcccctaac tggctatcct
     2641 ctttttcgcc gatcggcgtt tcaagagaaa tagttccctt ggaaatatcc aacatcatgc
     2701 ggaccttttc aggagttaaa cccatccgtt cggcaatttc ctctggagtc ggttcacggc
     2761 ctaattgttg caccaactgc cttgccgtcc gcgctaattt agtattggcc tcaatcatat
     2821 gtaccgggat acgaatggta cgagcctgat ctgcaatggc acgtgttaca ccctgtcgaa
     2881 tccaccacgt tgcataggtg ctaaatttat agccacgctg atactcaaac ttatcgacag
     2941 ctcgcattaa tccaatattg ccttcttgga tcaaatcgat aaactgcaat cctcgattcg
     3001 tatattttcg tgcaatactg acgaccagtc gcaaattggc ctcgatcaat tgggctttcc
     3061 ctcgctttac cttttcctca gcggtctcca gaatagtcag agcagccgta aattcttcca
     3121 tcggcatacc cagcacctcg tcctggaggc gcaagagcgt agctttcccc gcttcaactt
     3181 cttgttttaa tttttcaatt tcttccggag tcaggtgttg tttgcgcctc agggaactcc
     3241 cttttgtggt tacacgtggt tttttcccaa tcacctgaat accgtcctgc ccagcaagtc
     3301 caagcttttt cgtatgacgg gcagtcatca tttcaattga tcgaatttcc tgaccaacgt
     3361 ctttaacacg tcttagcatt tgatctttaa cccgctgatg cagattaaga gcttcgattt
     3421 gatccacaaa ttgatcggcc aggccaatcc ggcgcttctc aagttgtgcc tttgtatttt
     3481 tcgtcgtttg cccgtcccga ttttgggtgt aaagggcaag taatccacgc ccaagctttc
     3541 gaaccgttgc cagactctct agcgttcgga atcggagctc ctcatcttcc tgttcactgg
     3601 ccatggtctc ttcttcaact tcctcttcct ccagggtctc ctgtaggagc acgatatccc
     3661 gaacacggat ctcctcaagt ttcaattggt cgtggagtga ttccaggtaa tggatattca
     3721 gcggcatacc atagaccgca caggccaact cacgcttgcc ttcttcgatg tgtttcgcaa
     3781 gttcaatttc gccttcacgg gtgagcaatg gcacccgtcc catttctttt aaatacaacc
     3841 ggaccggatc atcgatacgg gtaggctcac ccggggtcaa atcaatatct agggaagaat
     3901 ccgtttcctc atcggaatca tctgaatcct cttgccccgc gttctttttt aaagctgcca
     3961 actgcgaggc cgtccgcttt gaaggatcaa tcacttgaag attgacttcg tgcaattcac
     4021 ctaaaatggt atgtaattga tcagaggtaa tctgatcggc aggaagaata tgcgttaagt
     4081 cttttatggt gacatgccct ttgggcttgc caatctcaat taatcgctga atcacctccg
     4141 gcaatgacac ggtcttcccc attaacatag ctccttcaaa attaatagct taacccaaat
     4201 caatcatacc acttttcgta ctgaaccaat gggcattcct ctcatgagga gaacctatcg
     4261 ctcttctctc gtcctttttc tcaacggacc acattattag aatgcagaat ggtgtgctat
     4321 cttttataaa gatttagaga ggaatcgcac atcagtctat cgactaagta cgtcatatcc
     4381 tccttgaggc gaaaccaggg aaaagaccaa ttgttaaaaa aaatcgaagg taaaaataca
     4441 atgcgcaatt tgataacact atataaaaat ttggactaca aagtaaagtt aaattttcta
     4501 agtgaataat aaaataactt aattcagagt acttattatc ttatctgaaa aatttatcga
     4561 aagttgccgc catacagcta tgactaatcg attgttttta ttaatttatt ttctattttt
     4621 gatcggaaaa gtgatttatg ggactagctc actaaaaggg acaaaatata cagaaactat
     4681 caagaaaata cggttatatc cgacaagatt acctaggacc accttctcga atctaaaaag
     4741 tctctggaca caagaacgag ctgaaatggg gagaacgccg caatcatgat ttcacttttg
     4801 ttggttgaag ataacccaaa agaagtgttg gttattaaaa atatgctgaa agaaggactc
     4861 caaaaccaat ttaccttgaa gcatagccgt tctctcagcg acgctctcga cctcattcaa
     4921 caaaatcagt ttcaagccat tattctggac tcgcatcttc cggacggcaa atcatttgaa
     4981 tccatcccac aattcctgca gttttgccct gatgccccga ttctgatttt gagcggcgtc
     5041 gaggaagaag accaagcgat tcaagccgtg aaaagtgggg tccaggacta tttaatcaaa
     5101 ggccagacca gcagctcgac tctctgccgg gccattcgct atgccatgga acgccagagg
     5161 gccacccgac gcattaccca gttggctcat tacgaccatc tgacaggact cgcaaaccga
     5221 ggtcttttct atgaacggtt aaattgtgcg gtggctcgct gtcatcggaa cgacacggca
     5281 atcgctctga tgtttttgga tctggaccat tttaaagaca tcaatgacag ccttggccat
     5341 gattgtgggg attccttact caaaaccgtg gccgcacgga ttaaaaaatg tatcagagaa
     5401 attgacacag gtgtccgatt aggaggagat gaatttgccg tccttctgga acaaattg
//
LOCUS       DATIAL010000376         6575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1036045, whole genome shotgun sequence.
ACCESSION   DATIAL010000376 DATIAL010000000
VERSION     DATIAL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036045"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /gene="mutS"
                     /locus_tag="VLA60_11710"
     CDS             complement(<1..1711)
                     /gene="mutS"
                     /locus_tag="VLA60_11710"
                     /inference="COORDINATES: protein motif:HMM:NF003810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HSF10073.1"
                     /translation="MKDQELSPLMRQFQDVKAQHPDAIVFFRVGDFYEMFFEDAEEAS
                     KLLGIVLTARGKAKGEAIPLCGVPYHTSTGYIAKLLKAGKIVALCEQVEDPKLAKGLV
                     RREVVRVYTPGTLYDHELLPAGEANFLSALCYTPLSSSGNTTLANRFGLASLDLSTGE
                     FWISESWMKHSLQSVVDELVRLEPKEVIFPAGIQEAMTSAFQSLPIARIVPRDATTFG
                     LEESKTILTKIFQVDRIEDLQLHGLHSGLQAAGGLLHYLADTQPTLVHAHIRRPWIRL
                     LEHEMQLDQTTLRNLEVLKPVSEQRQSPTLLTTLDKTKTPMGTRLLREWIVRPLTQVP
                     AIQLRQEAVKELVLHLGIRMTIREHLKTVQDLERLNSRIVLEVANPRDLMNLHRSLEN
                     LLLLQQLLPSLTSPMLQTIHEAWDPLQDVSSWIGDTIIPNPPLSTKEGGIIQTGVNAE
                     LDELRILAKEGTRLLAEMETRERNRTGIDSLKVKFNQVFGYYIEVTKANLSRVPADYH
                     RKQTLVNAERFTTGELQDLEGRLSSADQKMKNLEGQLFGEIRRRVASATIRIQGMAQQ
                     LARL"
     gene            complement(1879..2409)
                     /gene="tsaE"
                     /locus_tag="VLA60_11715"
     CDS             complement(1879..2409)
                     /gene="tsaE"
                     /locus_tag="VLA60_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HSF10074.1"
                     /translation="MNLNPSHSTSHQTATGNALQLRMASAQETIRFGERIGQQLTGGD
                     VLALTGDLGAGKTVLTCGIALGLGIPMDQVSSPTFTLIQEYPGTIPLIHVDLYRLEGP
                     SDLSTLGLEEYFTSNTIVLIEWAERFPQILPSDHLAIYLEYGEAENIRLVTLSGTGPN
                     TIRIVTNIQRNLSNPH"
     gene            complement(2416..3969)
                     /locus_tag="VLA60_11720"
     CDS             complement(2416..3969)
                     /locus_tag="VLA60_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HSF10075.1"
                     /translation="MWVVTAEQMQTLDRRTIQETKVPGITLMERAGTGAMRHLIEACG
                     STKGKKVVILCGKGNNGGDGLVVARLLAKKGAKLKVVLMAPIKALSPDAKIMYRRLSK
                     IIRPSLLTVNPSEESLHSLTQDADILVDALLGTGLSSAVRPPYSSAIGAMNASQAFTV
                     AIDIPSGLDSNTGAILGAAVQADLTVTFGCPKLGLYLGSAIDKVGSIQVVDIGIPQEF
                     VVDLNPQVHLLSQNMVRPLIPLRPLSSHKGTFGHAGIVGGSQGKTGAPAMAGLGALRI
                     GAGLATVATPQSISPILESKLLEVMTEPMPESSQHLLGMEAYPGLLAFVAKKSALAFG
                     PGMGTSPETTELLGHLLPQLEAPCVLDADALNGLAQQQQIFSALKRPPILTPHPGEMA
                     RLLGASSKQVNEDRIGVSRRFAMKHQVVLVLKGARTIIAEPQGQVAICPTGNPGMASA
                     GMGDVLTGIITGLLAQGLSGWDAARAGVYLHGLAGDLAAATIGEPGLIAGDVLTTIPH
                     ALTHTLSHS"
     gene            complement(4143..4856)
                     /locus_tag="VLA60_11725"
     CDS             complement(4143..4856)
                     /locus_tag="VLA60_11725"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HSF10076.1"
                     /translation="MARLGVNIDHVATLRQARGGHEPDPIVAASLAHLAGADGIVVHL
                     REDRRHIQDRDLTVLRETVQTHLNLEMAADDAVAKIALNIKPDLVTLVPERRQELTTE
                     GGLDVIEHRDRIQAMVQLLHEGGIPVSLFIDPDVNQLRAAHKVQADCIEIHTGRYANT
                     RRQQEEDVELDALVQTAKLASKLGLRVSAGHGLNYRNIRRLTGILEVEEFNIGHSIIA
                     HALFVGLERAVRDMKHLLG"
     gene            5108..6292
                     /locus_tag="VLA60_11730"
     CDS             5108..6292
                     /locus_tag="VLA60_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF10077.1"
                     /translation="MEPFYRIQRLPGYVFSQVQELKLQARKRGEDIIDLGMGNPDQPT
                     PRHIVDKLIETARLGRNHRYSASRGITKLRHAICDWYQRNYEVSLDPETEAIVTLGVK
                     EGLAHLALAMVGPGDVVLAPTPTYPIHMYSVIIAGGEVRGVELGPDSDFFENLKKAFK
                     QTQPAPRALIISFPHNPTTRVVDLEFFKKVVEFALEHQVWVIHDLAYADIVFDGYKAP
                     SLLQIPEAKQIGVEFYSLSKSYNMPGWRVGFCVGNREVIGALTKIKSYLDYGMFQPIQ
                     IASIIALNGPQDCVKEIVGRYERRRNALVRGLNRIGWPIDYPRATMFAWAKIPQPYLG
                     MGSLEFSKLLLRDAKVAVSPGIGFGEGGDDYVRFALVENEHRIFQAVRGIRQALKLDG
                     DV"
     gene            6292..>6575
                     /locus_tag="VLA60_11735"
     CDS             6292..>6575
                     /locus_tag="VLA60_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSF10078.1"
                     /translation="MRKTIQVGLIGFGTVGTGVVKILQENVSLITKRVGVPVALVRIA
                     DLDVKTDRGVSVPQGVLTTDVRDILDDPAIDIVVELIGGHEPAKRFILQAL"
BASE COUNT         1525 a         1575 c         1830 g         1645 t
ORIGIN      
        1 ccagcctggc cagttgctgc gccattccct ggattctgat ggtggcagat gcgacacggc
       61 gacggatttc accaaataac tgcccttcca ggtttttcat cttttggtct gcgctcgaga
      121 gccgaccttc gagatcttgt agctctccgg tagtaaaccg ttcggcattg accagggttt
      181 gtttccggtg gtaatcggca ggaactcgcg ataaattcgc tttcgtcact tcaatgtaat
      241 agccgaagac ctgattgaat ttcaccttga gggagtcgat gccggtccgg ttgcgttccc
      301 gagtttccat ttccgccaat aacctggttc cttcttttgc cagaattcgc aactcatcca
      361 gttctgcatt cactcctgtt tgaataatgc ctccctcctt ggtcgaaagg ggtggattgg
      421 gaataatggt gtcgccaatc caggatgaga catcctggag gggatcccac gcctcatgga
      481 tggtttgtag catgggagac gtgagagacg gtaagagttg ttgcagtagc agcagatttt
      541 ccagggagcg atggagattc atgaggtctc gagggttggc cacttccagg acaatccgac
      601 tattcagtcg ttcaaggtct tgtaccgttt tcagatgctc acgaatcgtc atgcgaatcc
      661 caagatgaag gaccaattcc ttcacggctt cctggcgtag ttgtatggcc gggacctggg
      721 tcaggggacg gacgatccac tcccgtaaaa gtcttgtgcc cattggagtt ttggtcttgt
      781 ccaatgtcgt gagtaaagta gggctttggc gttgttcgga tacgggcttg agaacttcta
      841 aatttcgtag ggtggtctga tccagttgca tttcatgttc gaggagtcga atccatggac
      901 gccgtatatg ggcatgcacg agcgtgggct gagtgtcagc caggtagtgt aaaaggccgc
      961 ccgcagcttg aaggccggaa tgaaggccat gtagctggag gtcttcgata cgatcgactt
     1021 gaaaaatttt ggtaaggatg gtcttgcttt cctcaaggcc aaaggtcgtg gcatcacgag
     1081 gcacgatacg ggcgataggc aaggattgga atgcggaagt catggcctcc tgtattccag
     1141 caggaaagat gacttctttg ggctcgaggc ggaccagttc atcgacgaca ctttggagcg
     1201 aatgcttcat ccaggactcc gaaatccaaa attccccggt tgaaaggtct agggaggcca
     1261 gtccgaatcg attggctaga gtggtattcc ccgatgagga taacggggtg taacacagcg
     1321 ctgacaggaa attggcttct cctgccggaa gcaactcatg atcgtataag gttccagggg
     1381 tatagacccg cacgacttca cgacggacga gccctttggc aagcttgggg tcttcgactt
     1441 gttcgcataa ggccactatt ttcccagcct ttaagagctt tgcgatatac ccggttgaag
     1501 tgtgataggg gactccgcaa agagggatgg cctccccttt ggccttgcct cgtgctgtta
     1561 atacgatgcc aaggagtttt gaggcctctt cggcgtcctc aaaaaacatt tcataaaaat
     1621 ccccaacacg aaaaaataca atggcatcag gatgttgagc ctttacatcc tgaaattggc
     1681 gcatgagcgg ggagagttct tggtctttca tcaggtgagc tgtttcgttt tgataattgt
     1741 tgagtgtggt aagaatcgtc tgaaatgagt gtgctggtta tgtcattgtg ggtcgatgcc
     1801 gcgccttcgt tattgaattc ggatcggatg gccgcacgaa tatgtcggtt cagttgtatg
     1861 cggtaccgat aagttgagtc aatgcgggtt tgataaattt ctttgtatgt tggtcactat
     1921 gcggatcgtg ttgggtccgg ttccggacag ggttacaagg cggatgtttt ctgcctctcc
     1981 gtattccaag taaatggcca gatggtcgga gggtaatatc tgagggaacc gctccgccca
     2041 ttcaatgagg acaatggtgt ttgaagtgaa atattcctcc aatcctaatg tggatagatc
     2101 ggatggcccc tcaagacgat ataaatccac atgaataagc ggaatagtcc ctggatattc
     2161 ttgaataagg gtaaacgtgg gactgctgac ttgatccatg ggaatcccta gcccaagggc
     2221 gataccacag gtgagaacgg tttttcctgc tcccaagtca cccgtcagag ccaacacgtc
     2281 tcctcctgtt aactgttgcc ctatccgttc accgaatcgg atggtttcct gggcagatgc
     2341 catccgtagt tgaagagcat ttccggttgc ggtctgatga gatgtggagt gagagggatt
     2401 cagattcatg aattcctacg aatgagacag cgtgtgggtc aatgcatgag gaatggtggt
     2461 caacacatcc cccgcgatca gtcctggttc accgatggtg gcggccgcca gatcgcctgc
     2521 caatccatgc agatagacgc cagcccgcgc cgcatcccaa ccgcttaagc cctgagctaa
     2581 taaaccggta atgattccgg ttaggacatc tcccattccg gcggaagcca ttccaggatt
     2641 ccctgtcgga caaatggcta cttgtccctg gggttcggcg atgatcgttc gagcgccttt
     2701 taataccagg accacctgat gtttcatggc aaaccgtctg ctgacgccga tgcgatcctc
     2761 attgacttgt ttggatgagg ctccaagaag cctggccatc tctccagggt gtggggtcag
     2821 gattggcggt cgtttcaagg ctgaaaatat ttgctgctgt tgggctaatc cgttgagggc
     2881 atcggcatcg agaacgcagg gtgcctccag ttgagggagg agatggccca agagttcggt
     2941 tgtttcggga gaggtcccca taccgggtcc gaaagccaga gccgattttt tagcgacgaa
     3001 agcaagaagg cccgggtagg cttccattcc cagcaggtgt tgggacgatt ccggcatggg
     3061 ttcagtcatc acctccagga gctttgactc caaaatgggg gaaatgcttt gaggagtagc
     3121 cacggttgcc aggccggccc caatacgaag tgcccctagt ccagccatag ccggagcccc
     3181 cgttttccct tgtgaaccac caacgattcc tgcatgtcca aaggtcccct tgtgtgagga
     3241 cagggggcga agaggaataa gggggcgaac catattttgt gacagaaggt gaacctgggg
     3301 gttcagatcc accacaaatt cctggggaat cccaatatcg acgacttgga tgctaccaac
     3361 tttgtcgatg gctgagccca gatacaggcc gagtttcggg catccgaacg tcacggtgag
     3421 atcggcctga acagcggctc ccagtatggc cccggtgttg ctatccaagc cggacggaat
     3481 atctatggct acggtaaagg cctgcgaggc attcatggct ccgatggcgg aggagtaggg
     3541 tgggcgaact gcggaagaga ggcccgtacc aaggagcgca tcgacaagaa tatccgcgtc
     3601 ttgggtgaga gaatgcaggg attcttccga gggattgacg gttaacagag atggtctgat
     3661 gattttgctg agtcgtcgat acataatctt cgcatcggga ctgagtgcct tgattggagc
     3721 catcaagact accttcagct ttgcaccttt ttttgccaag agcctggcaa caaccaaacc
     3781 gtctccacca ttattcccct tgccacaaag aatgacgacc tttttgcctt tagtggaacc
     3841 acaggcttcg ataaggtgcc tcatcgcacc ggttcccgcc cgctccatta aggttatgcc
     3901 gggaaccttg gtttcttgaa tcgtgcgccg atcaagtgtt tgcatctgtt cagcggtcac
     3961 gacccacata ggcaaatcat agaaacaagg agaaatggtt ggttaaagca tgagcaaccg
     4021 caagccatgc gtagacgggt taggatgaca ttcgggtccg acactgcttt tgaattgact
     4081 cgtggatgtc tccggatcat ttaccgtgca cacagatggg aacctgtttc ttttcataga
     4141 cgttaaccca ggaggtgttt catgtcacgg accgctcgtt caagtccgac aaacagggca
     4201 tgagcgataa tgctatgacc gatgttgaat tcttcaactt ctaaaatgcc ggtcagccgc
     4261 ctgatattcc ggtaattcag cccatgcccc gcactgacac gcaatcctaa tttcgacgca
     4321 agtttggccg tttgaaccaa cgcgtcaagc tcaacatctt cttcctgttg acggcgggta
     4381 ttggcgtacc gaccggtgtg gatttctatg cagtctgctt ggaccttgtg ggcggcgcga
     4441 agttgattga catcaggatc aatgaacaga ctgacgggaa ttccgccttc atggagtaat
     4501 tgcaccatgg cctggattcg gtcccgatgc tcgatcacgt ccaacccacc ctctgtggtg
     4561 agttcctggc gccgttcggg cacgagggtc accagatcgg gtttaatatt cagggcaatt
     4621 ttggccacgg catcgtcagc ggccatttct aagttgagat gcgtctgcac ggtttcacgc
     4681 aaaacggtca gatcccgatc ctgaatatgt cgccggtcct cccggaggtg cactacaatg
     4741 ccatcggccc cggcaaggtg agccaacgaa gccgcgacaa tggggtctgg ttcatgtcct
     4801 ccacgagctt ggcgaagagt cgccacatgg tcgatattca cgccgagtcg tgccatggaa
     4861 gtcctttaag gtccgggcga gtgatgaatc aacgagatcc cattatggca aaagggaaaa
     4921 tcttcctgca ataaagggat tttcagttca gggtgagtga ggcggggaaa attgactgaa
     4981 aattcccata ctctgaaatt gactggtcgc gttacctcat ctagaataaa aactctatta
     5041 aatcgtacga ttatctatcc atttccttcc caaaccatca aggacgattc gtcattttac
     5101 acaaacaatg gaaccttttt accgaattca acgtctgcca ggatacgtct tctctcaggt
     5161 tcaggagtta aaactccaag cccggaagcg gggagaagat atcattgatt taggcatggg
     5221 caatcctgac cagcccaccc cccggcatat tgtagacaaa ttgattgaga ccgcgagact
     5281 ggggcgcaat catcgctatt cggcttcacg cggtattacg aaattgcgtc atgcgatttg
     5341 cgattggtat caacgaaatt acgaggtcag tctcgatccg gagacggaag cgattgtgac
     5401 ccttggggtg aaggaagggt tggcgcatct ggccctggcc atggtgggac cgggtgacgt
     5461 agttctggcc ccgaccccca cgtacccgat tcatatgtac agtgtgatta tcgcaggtgg
     5521 tgaagtgcgc ggggttgaac tcgggccgga ttcggatttt ttcgaaaatc tgaaaaaggc
     5581 cttcaagcag acgcagccgg ctcctcgggc gttgatcatc agttttcctc ataatccaac
     5641 cactcgcgtc gttgatctgg agtttttcaa aaaggtggtg gagtttgccc tggaacatca
     5701 agtctgggtc attcatgatt tggcctatgc cgatatcgta ttcgatgggt acaaagcccc
     5761 cagccttctt caaattcctg aggccaaaca aattggcgtg gaattttact ctctctcgaa
     5821 gagttataat atgccggggt ggcgggttgg attttgtgtc gggaatcgtg aagtcatcgg
     5881 tgcgttgacc aagatcaaaa gttatctgga ttatggcatg ttccagccta ttcaaattgc
     5941 cagtattatt gccctgaacg gtccgcagga ttgcgtaaaa gaaattgtgg gacgatatga
     6001 gcgacggcgc aatgctctgg tccgcgggct caatcgcatt ggttggccga tcgactatcc
     6061 tcgcgccacc atgtttgcct gggcaaaaat cccacaaccc taccttggga tgggatcgct
     6121 ggagttttcc aagttattgt tacgagatgc caaagtggcg gtgtcaccgg gaatcggatt
     6181 tggcgaaggc ggcgatgact atgtgcgctt tgcattagtc gaaaatgagc accggatttt
     6241 ccaggccgtt cgaggaatcc gtcaggcctt aaagctggat ggagacgtgt aatgcgaaag
     6301 acgatccagg ttgggctgat cgggtttgga acggtgggaa caggagtcgt aaagattctc
     6361 caggaaaatg tcagcctcat cacgaaacgt gttggagttc cggtggcact cgtgcgaatt
     6421 gcggacttgg atgtgaaaac cgatcgcggg gtttcggtgc ctcaaggggt acttacgacg
     6481 gatgtcaggg acattctgga tgatccggct attgatatag tggtggaatt aattggcggg
     6541 catgaaccgg ccaaacggtt tattcttcag gcgct
//
LOCUS       DATIAL010000377         4266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_177011, whole genome shotgun sequence.
ACCESSION   DATIAL010000377 DATIAL010000000
VERSION     DATIAL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177011"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VLA60_11740"
     CDS             complement(<1..481)
                     /locus_tag="VLA60_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HSF10079.1"
                     /translation="MLAGQASWFKNPFRRFFGIGSQTNESDETSYTSKEILVDLTVGI
                     HLAQDMALMWSERFHQVRVGDGIINTLPEIQEGFPTINGINGADIWGHKLTFRYDTRD
                     RQLISSQGTYVNVSVEWNRNFKPQPVTDWWRTTVDARHLVPHFHNRLVFVSHLYADTV
                     "
     gene            complement(1051..3330)
                     /locus_tag="VLA60_11745"
     CDS             complement(1051..3330)
                     /locus_tag="VLA60_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HSF10080.1"
                     /translation="MMNRFLGPHNLFRLFKHPRSFAYLLSCWWVILIGIQQTERWFLL
                     PHVIASEPPTISLLGKTLLVGVLSDGVTASAGILLAMILAMSGIPFCLILERPPGSNR
                     MISVYRRTLQWTLILIGALFLISAMVDVSYYRLFHHHLNFPFFEYLDEAFQASRHHTE
                     SQGSNQTLAELAEAGSWLRYLITFFIILALTILTGIWGMNRLWSHSIFAYAQTTHFQF
                     RAVLFAVPFAVGAAAWSPLDLPQAFTLQIDSTAYHGLAQNPILSASLPLQEYIRSQGK
                     WTPAQLPRPMSANEALTVTHQILAPSARWPSFQFPLLKEQSTIPPFTLPSRVNILVLL
                     VEGLDRRFLGKTLSLSNPAPEGTNLPSRISLTPFLDSLRQDSVYFEHFFSNGVQTTRG
                     LLATLCSVFPRQGTAVIKTRNTHEYLCFPSVLQKAGYRTEMMMGLDSDIPGSRIFLAR
                     NGIEQISGGQDFPADVERMGIGLTDGAFLDVLYQRVAKLQQQPDPYFLAALTTGTHHP
                     FTIPLRHPEVRALKDQPDPYLAALRNFDLEFSRVFTHLKRDGLLTNTILFVLGDHGRH
                     EPVGFTEGERVSGHFSTPLFVWMDETLRTQLQIVPHTVTTVGSQVDIAPTILALTGLT
                     PNLTPFMGRDLSCLLIKNCLSDNLAFLSNMYDDAIGIADHQGVWWYAFDTGLLNHTDL
                     DLQTPVKHSSLDEIDAAHAYRSMISLYLTANTLIEHNRIWSWGEFDTPFTPPGALHAL
                     SPNRVSASTPVETRLFQYE"
BASE COUNT         1127 a          958 c         1183 g          998 t
ORIGIN      
        1 tgacggtgtc tgcgtacaaa tgggatacaa aaaccaggcg gttatgaaaa tgtgggacga
       61 gatggcgggc atcaacggtc gttcgccacc agtccgtcac gggttgcggt ttgaaattcc
      121 gattccattc gacggacacg ttcacatagg ttccttgcga ggaaattaat tggcggtccc
      181 gagtgtcata acgaaatgtg agtttatgcc cccaaatatc tgctccgtta atgccgttaa
      241 tggtcggaaa cccctcttga atttctggaa gagtattgat gattccatcc ccgacgcgta
      301 cctggtggaa ccgctcgctc cacataaggg ccatatcctg agccaaatgg atcccgacag
      361 tgagatccac cagtatttcc tttgaagtat aagaggtttc atcggattca ttcgtttggc
      421 ttccgattcc aaaaaatcgt cggaagggat ttttaaacca ggaggcctgt cccgccagga
      481 tataccgccc ccccctgccc caacattttt gtaagagaga tcgatttcac gctgaacctt
      541 ggtggagtag gacaatatcg cacggtattg cgtggtatca gacgggtatc catagtaatt
      601 catggtcaat gtttcaccga tatacttatt atgcaaatat tggggagcga taatgtcttc
      661 taaatggccg ttcgcatttg atcggagcat tgggacgagg aaaccgtaat aggccccttc
      721 attgcggctg tagccgaaag ccgggaccgg aaacacggaa aattctccct tggtcggaag
      781 agacctgatt gcctcctctt tggcttcttc ctgtttcgct tcccctctct gtccttccgt
      841 accctgtact ggaaaatccg ggtcgtgctt gaatcgttcc tgccgatttt gagaggcctg
      901 atcgggatca ggagcgtttt gccccagggt ggtttctgga aaaaaccccg ctccgcacat
      961 cacaaaaatc acgaaagaaa cgatcttgtt caaagacccg gaaaaatgtc ggtgcatggt
     1021 tcatggtttt cggatgagtg gtcatgatgt tcattcatat tggaaaagac gggtctctac
     1081 aggagtcgac gcagagacgc gatttggcga tagggcatgc aaggctccgg gtggggtgaa
     1141 tggtgtgtcg aattcccccc acgaccaaat ccgattgtgt tcgatgagag tattggcggt
     1201 gaggtaaaga ctgatcatgg aacggtaagc gtgcgccgca tcaatttcat caagggagga
     1261 gtgcttgacg ggtgtttgca ggtccagatc ggtatggttg agcaatccgg tatcaaacgc
     1321 ataccaccag accccttgat gatcggctat tccaatcgca tcatcataca tattgctgag
     1381 aaacgccagg ttatccgaca aacagttctt gatgaggagg caactcaaat ccctccccat
     1441 aaagggcgta aggttcggtg tgaggccggt gagggccaat atcgtcggtg caatatctac
     1501 ctgactgcca acggttgtca cggtgtgggg cacgatctgg agttgtgttc taagcgtctc
     1561 atccatccag acgaacaaag gggtcgaaaa atgtcctgac acgcgttcgc cttccgtgaa
     1621 gcctacaggt tcatgtcggc catggtctcc caggacgaag agaatggtgt tggtcagcag
     1681 cccgtcacgt ttcagatggg taaacacccg cgaaaattcc agatcaaaat ttcgaagagc
     1741 tgccagatag ggatcgggct gatcttttaa tgcccgaact tcagggtgcc gcaagggaat
     1801 cgtaaaagga tgatgagttc cggtggtgag ggccgcaagg aaataggggt cgggctgctg
     1861 ctgcaatttg gcgacgcgtt ggtacaggac atcaaggaat gcgccgtccg tgaggccaat
     1921 gcccattcgc tccacatccg cgggaaagtc ctgaccaccg gagatctgct cgattccatt
     1981 tctggctaaa aatatacgtg aacctgggat atcgctatcg aggcccatca tcatttccgt
     2041 ccggtacccg gccttctgga gcacagaggg aaagcagaga tactcatgcg tgttgcgcgt
     2101 cttgatgacg gcggtacctt gcctcgggaa tactgagcat agggtagcca ggaggccccg
     2161 ggtggtttgc accccattgc tgaaaaaatg ttcaaaatac acactgtcct gccgcaggga
     2221 atccagaaat ggagtgagag agattcttga gggaaggttc gttccctccg gtgccggatt
     2281 cgaaagggat agggtttttc cgagaaatcg tcgatccagg ccttcaacca acagtacgag
     2341 gatattcact cgtgagggaa gcgtaaatgg cgggatggtg gattgttctt tgagcagggg
     2401 aaattgaaag gatggccacc gtgccgatgg agcaagaatc tgatgagtga cggttaaagc
     2461 ctcgttcgca ctcatgggcc ggggaagctg cgccggggtc cacttcccct gggatcggat
     2521 gtactcttga agcggaagtg aggcagagag aattggattt tgagctaaac catgataagc
     2581 cgtactatca atttgcaggg taaaagcctg tggaagatcc aaaggactcc aggcggcagc
     2641 tccaacagca aatggaaccg caaacaggac ggcccggaac tgaaagtgag tggtttgggc
     2701 gtaggcgaat atcgaatgag accaaaggcg attcattccc cagattcctg tcaggatggt
     2761 caaagcgagg ataatgaaaa atgtgatgag ataccggagc caacttccag cttcagccaa
     2821 ttccgcgagg gtttggttgg agccttgaga ttccgtgtga tgccggctgg cttggaaggc
     2881 ttcatccagg tattcaaaga aaggaaaatt gaggtgatgg tgaaacagac gatagtaaga
     2941 tacgtccacc atggcgctta tcaagaatag tgcacctatc aatatgagag tccactgaag
     3001 cgtccgtcga tagacggaaa tcatacgatt ggagccagga ggtctttcca gaatcaggca
     3061 gaagggtatg ccggacatgg cgagaatcat agccagaagg attcccgcac ttgccgtcac
     3121 gccgtcactg aggaccccca cgagaagagt tttgcccaag agactgattg tcggtggttc
     3181 gcttgctatg acgtggggaa ggagaaacca ccgttcggtt tgttggatgc cgataagaat
     3241 cacccaccag cagctcaata gataagcgaa cgaccggggg tgcttgaaaa gccggaaaag
     3301 gttatgtggg ccaagaaatc gattcatcat tcctattcca aatgattgcc ggattatgag
     3361 gggtggatag tccgagactc tcgtgctaac tggctcttga ggaaagctgt cctcccgcta
     3421 tcttctgctt gagctggaaa aaatggccgg tgttcattca gtatgatcaa aagaccgggc
     3481 atcgtctgtt caaaattgct cataatcagc ccaggccata acgttctgaa gcggggaatg
     3541 tcgtgcgagt cccaccgatg gctataagtg gtctcaatta ggaaggatcc caccacaaca
     3601 tagaccgaca aatgggctag ctcacagaag gccattcaaa aaagatgtgc tgtctggtgc
     3661 taggaggcct gaagggcaaa ccaatgacac tcgccaaaca gaccaccgca taaattgatc
     3721 cacagacgac agaagataag cacagcatgg tgagggttga aaacgtaggc acacaatcgg
     3781 ctatgaaact ggtccttatt caatggcttc tggtatggac gggttcagca caatcacgcc
     3841 agattgcaga acggaaagaa tcgaggagaa attcaattgt ttatgaaaaa taaaacggga
     3901 aggaaatcga acggatgaag gtagaaatga aaaagttttg tatgaaaatg aaaaatttat
     3961 tgacgaaggt taagatgcct tcaggattgc gtctgtccaa ggagaaagtg ctgacgcttt
     4021 gttatacagg gtcgggggaa aaaagataaa aggtcttcga gaatcttcgc tgattctgca
     4081 gcggggagtc gtcatgaagc cacctttggt ttattccagg ctgggaagag cgtcaagaag
     4141 gatttgggct tcctgctgat tattctgttg cctggattcc aatgccggcg atcgagcagg
     4201 tttatctagt atgtgacgca gttctttccg tgcagcacga tagttctgcc ttttgatata
     4261 ggtttt
//
LOCUS       DATIAL010000378         3421 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1467892, whole genome shotgun sequence.
ACCESSION   DATIAL010000378 DATIAL010000000
VERSION     DATIAL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467892"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..872)
                     /locus_tag="VLA60_11750"
     CDS             complement(96..872)
                     /locus_tag="VLA60_11750"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF10081.1"
                     /translation="MSSFQTGKPLAPWSRSFSPRLLMGGHTKFDELPSQASPYLGLEV
                     EMHVQAVAGDAGMIPVDLERALDLGIEVFLLSEGCAVESLCIAALWRHSLRTGYLAAR
                     IALNQGSNPRMVWQSFVGGVLHDIGMLVFLTQHLEAFTTVVEIAQCRGGSLAKLERRV
                     LGYTHGESGATFLARWGIDDVLLAIAAFHDDPWNAPHATFGPLTAVYAGNFVEGGGIA
                     QDGDGVIGREGEAYLTRLGLWDDLPIWQSWMPSIPQLAIH"
     gene            complement(1160..3007)
                     /locus_tag="VLA60_11755"
     CDS             complement(1160..3007)
                     /locus_tag="VLA60_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF10082.1"
                     /translation="MVPVSAHRPVLLVGTHPETQEMLRDALSVPSMDALPVENLSSDK
                     RVASSGAQPSLPQFTLVSVEMGSQVLAKFAEAQAANNPFALVVIEGRSGEWEIVRDLV
                     EGLWKCDQTLRCIFCLPSDRASWPHRLVVSRPEQWAVLRIPFLGEEAFQLACCLSLPL
                     PKPMEPAFDRATDSDETGCSSTPYMDIDTDNGEEPTGALESARGELAASRYYVENILR
                     SMADSLLVINADMTIGAVNPSLLNLLGYQEDELIGQSPGLIFGEEFSQGVIIENLLLQ
                     GSVSGVESSFLTHEGQKITISVSGSMMQDLQGQFQGLVCVAQDITERKRMEEEKLQLH
                     EQLMETSRQLGMAEVATGVLHNVGNVLNSINVSIGVITDLLRNSMVGDVGRISQLLDK
                     HREDLGTYLSQNPKGKQVPHYLGKLSGQLKEEQRVALLELERLRENVGHAQQCVAAQQ
                     DLAKPNGMTEQVCVAEVVAEALAVNQKMLEETNVAVIQEFQEVPQLIVDKHQLLQILV
                     DVIRNACQAMESAAQRHLVVRIKLIIGPPDSLCLEVQDTGSGIPPDDITKIFGQGYST
                     KYGGRGLSLHHGALMAKNMGGALRAQSDGMGQGATFFLDLPGNFHFPDL"
     gene            complement(3106..>3421)
                     /locus_tag="VLA60_11760"
     CDS             complement(3106..>3421)
                     /locus_tag="VLA60_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10083.1"
                     /translation="GVPPVIRITATIIQDRRHTGLGQHGKLCQIEIHDQGIGIPPEQF
                     ENIFGMFKRLHRKEEFEGKGIGLAMCQRIVEQCGGAISVRSKLGEGSTFIVTLPFSDE
                     RS"
BASE COUNT          922 a          993 c          718 g          788 t
ORIGIN      
        1 ctaacgaagg taaatgagaa atatctgcga aaaacatggg aggggcattg ggggtcatgc
       61 gaaccgtagg acaatgaatt gagcgatacg agagttcaat gaatcgccag ttgcggaata
      121 cttggcatcc agctttgcca aatcgggaga tcatcccaca gccctaaacg ggttaaatac
      181 gcttctcctt ctctcccaat gactccatcc ccatcctgag cgatccctcc tccctcaaca
      241 aaatttccgg catacaccgc cgtcaaaggg ccgaaggtcg catggggagc gttccatgga
      301 tcatcatgga acgccgcaat cgccagtaac acatcatcta taccccatcg cgccagaaaa
      361 gtggctccac tttccccgtg agtataccct aagacccgcc tttccaattt tgccaagctc
      421 cctcctcggc attgagctat ctctacgacc gtcgtaaatg cctccagatg ctgagtcaga
      481 aatacaagca tgccaatatc atgaagcacc cctcccacaa acgattgcca gaccatgcgt
      541 ggattcgatc cctgattcaa ggcaatccgt gcagccaggt atcccgttct gaggctatgc
      601 cgccacaagg ctgcaataca caaagactct actgcacatc cctcggacaa gagaaacact
      661 tcgatcccaa ggtctaacgc acgctccaga tcaaccggga tcattcccgc atcacccgct
      721 actgcctgca catgcatttc cacctcaagg ccaagataag gtgacgcctg agatggaagc
      781 tcatcaaatt ttgtgtgccc tcccatcagc aaccggggtg aaaaagatct ggaccagggg
      841 gccagtggct ttccagtttg aaacgaagac acccgatttt catgagtcct ttctccctcc
      901 cccttccaag agtcatcatt ggtttgacgc caatattcaa tcacgggaga tactccagga
      961 aacacagaaa aattgcagag gactgaaagc ttcctctcac accttcaaaa ttttctcggt
     1021 ttcaccagac tgactcttga ggtaggaatc cgtgagacag tccatgaaaa atgccaaaca
     1081 aaaggaagag gcaacagaac cacacaacgc tcatgaagag acggtcagaa aaaacgattg
     1141 aattcgttgg atgaagaagt cacaaatcgg gaaaatgaaa attccctggc aggtctagaa
     1201 aaaatgtggc gccttgcccc ataccgtcac tttgagcgcg aagcgccccg cccatatttt
     1261 ttgccattaa cgcaccgtga tgcagactca agccccgtcc tccatatttg gtgctatacc
     1321 cttgcccaaa aattttcgta atgtcgtccg gaggaatccc gctgccggta tcctgaactt
     1381 ccaagcaaag agaatccgga ggaccaatga tgagcttgat acgcacaact aagtgcctct
     1441 gagcagccga ttccattgcc tgacaggcat tgcgaatgac atccaccaaa atttgaagga
     1501 gctgatgctt atccacgatg agctgtggca cctcctgaaa ctcctgaatc accgcaacgt
     1561 tggtttcctc caacatcttt tgattcacgg ccagggcctc cgctaccacc tcagccacac
     1621 atacctgttc cgtcatcccg ttgggcttag ccagatcctg ttgggccgca acgcattgct
     1681 gagcatgccc gacattctcc ctcaagcgct ccagttccaa caatgccact cgctgctctt
     1741 cctttaattg ccccgataat ttccccaaat aatgcggtac ttgtttcccc ttggggtttt
     1801 gcgataagta cgttccaaga tcttcacgat gtttatccaa taactgtgag atccgaccta
     1861 catcccccac catcgaattt cttaaaagat ccgtgatgac cccgatggat acattaatgc
     1921 tatttaacac attgcccaca ttatgcagca cacccgttgc cacttcagcc attcccaact
     1981 gtcttgaggt ttccattaac tgctcatgca attgcagctt ctcttcttcc atccgttttc
     2041 gttcagtgat atcctgggca acacatacca acccctgaaa ttgcccctgc agatcctgca
     2101 tcatcgaacc agagaccgaa atggtaattt tctgtccctc atgagtcagg aaactggatt
     2161 ccaccccact caccgatcct tgaagtaaga gattttcgat gataacacct tgggaaaatt
     2221 cctcgccaaa aatcaagccc ggcgactgcc caatgagctc atcctcctga taacccaaaa
     2281 ggtttagcaa agaaggattc accgctccaa tagtcatatc ggcgttaatg actaacagag
     2341 agtcggccat cgatctgaga atattctcaa cataatatct ggaagctgct aattcgcccc
     2401 gagcagactc caatgccccc gtcggctctt cgccattatc ggtatcaata tccatgtagg
     2461 gggtagagga acaaccggtt tcatcagagt cggttgccct atcaaacgca ggctccatag
     2521 gtttcggtaa cggcaggctc aaacaacagg ctaattgaaa ggcctcttct ccaagaaatg
     2581 ggatccgaag taccgcccat tgctcgggcc ggctcacaac cagtcgatgg ggccaggatg
     2641 cgcgatctga gggaaggcag aagatgcacc gtagggtctg atcacatttc cacagcccct
     2701 caaccagatc tcgcacgatc tcccattccc ctgaacgacc ctcaatcaca acgagagcaa
     2761 aaggattgtt cgcagcttga gcctctgcga acttcgctaa gacctgagac cccatttcca
     2821 cgctgacaag ggtgaactgc ggtaaagatg gctgggcacc ggagctcgca acacgtttgt
     2881 cacttgataa gttctctacc ggcagggcat ccatagacgg cacagacaac gcatcacgca
     2941 acatctcttg ggtttcggga tgcgtcccaa caagtaagac agggcgatgc gcagaaaccg
     3001 gtaccataga ttataatgat ctccccaata tccacaaagc caatgtcagc aatatatgct
     3061 tctcccaatc ggtaaagccc tgtgggtcat ctttgggact ggccgtcaac tcctctcatc
     3121 gctgaatggg agcgtgacta taaaggttga tccttctccc agcttggaac gaacggagat
     3181 ggctccaccg cactgctcga caatacgttg acacattgct aacccaatcc ctttcccttc
     3241 gaattcctct ttccgatgaa gtcgcttaaa catcccaaaa atattttcga actgttctgg
     3301 cggaatacca attccctgat catgaatttc gatttgacaa agcttcccat gctggcctaa
     3361 acctgtgtga cgtcgatcct gaataatcgt tgctgtgata cgaatgacag gtggcacccc
     3421 c
//
LOCUS       DATIAL010000379         3033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_929354, whole genome shotgun sequence.
ACCESSION   DATIAL010000379 DATIAL010000000
VERSION     DATIAL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929354"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(609..932)
                     /locus_tag="VLA60_11765"
     CDS             complement(609..932)
                     /locus_tag="VLA60_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10084.1"
                     /translation="MAEEKQKIDLSMYGVAEIIKWCITRNNGRIPGTDTAVFKALQAA
                     LAEKPTTGDYFTLDQFWKTRKVFEFTKDEVAVVDRCLYDLPNFDGAQLPQIRYKFWPE
                     AVCSK"
     gene            1131..2381
                     /locus_tag="VLA60_11770"
     CDS             1131..2381
                     /locus_tag="VLA60_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSF10085.1"
                     /translation="MVYPASIIAPSQPTYSGERQEELYSSQSLWARLVIPLGLLGLLS
                     LVGCPTREEYHPPDLIYHYLDIPVGKNPTSIRASDFNGDGFTDLITSNIQGNSLSLLL
                     GKGDGSFQDQKTISACQEPRNVAVDDFNLDHVPDFVVACSGDNHALVFLGQGDGTFLR
                     GAQYLVHRTPVSIATGDFNEDFLPDLAVALRNDKIQILLGMGNGKFRLGPLYEYGDTP
                     TSVATEDLNGDKHLDLAVSNGGPMSHAVSIWLGLGDGTFQAPTDYRTGKRPLGVSFGD
                     FNTDRAIDLLVINGEMNTITVFLGNGDGTFQEGRESGGDAGPNFGVAQDFDGDKIPDV
                     AVANIQSGSISLLFGKGDGTFHYPPRDYPTPHAPFALTTLSIATGRGEEPGLAVVNNA
                     SSNVSIFLHHGLNIRKGQLPATPS"
     gene            2458..2700
                     /locus_tag="VLA60_11775"
     CDS             2458..2700
                     /locus_tag="VLA60_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10086.1"
                     /translation="MTPVRSVIQPREENVRTFGWWFGMGSLLTLAVLLVQGGMRDLIY
                     GSSYSGTWEGILSMGLTVFGGGVLAGCVAVILNRLR"
BASE COUNT          709 a          669 c          776 g          879 t
ORIGIN      
        1 tgaatgttct ggctcgtcag atgaatcggg atcgattccc gccagaaggg tttgcatttt
       61 tcatgtcaca ggagccggtc gtcctggctc ctggccagat tctgactgat gatttctccc
      121 cgctagattt acttcagggg gaagggtaag catccctttc gcttcgagtc agcagaatcc
      181 cttccctcat tcataaaaac gacctaggat acctctccgc ttgctgagga gggtttcacg
      241 agaaaggtga cctgttcctg gttcttacgg tgggaaaaat ccgggtgttg taaaaaccgt
      301 aagatgccac tctaaccaaa ggagggatcc actcatgtgg cgtctggcgc ggactgcttt
      361 ggcgtttttg attgccaaga gttattttgt ctttggcggt tttcactccc cacgtttacc
      421 aggtgtccct aatcccaatg tataggtgta taccagaatt gctggtttga accgtccaac
      481 cctagcttga ttttggccgt ctggaagcgt tgttgcgccc attctaagcc tgaagacaag
      541 gattgtctgg agacaggcga tcgaaggaga taacaatatg ttcaaaaagt atggggcctg
      601 atgtcatcct attttgaaca gacagcctct ggccaaaact tgtatcgaat ttgtggtagc
      661 tgagcgccgt caaaattcgg gagatcataa agacatcgat caaccacggc tacctcatct
      721 ttggtaaatt cgaagacttt ccgtgttttc caaaattgat ccagtgtaaa ataatcgcct
      781 gtggttggct tctccgccag tgcagcctga agtgctttga agacggcggt atcagttcct
      841 ggaatacgtc cgttgttgcg ggtaatgcac cattttatga tttcggctac cccatacatg
      901 cttagatcaa ttttctgctt ttcttcggcc atgatttcct ccttacaaac agacaaaaag
      961 acaagggacc tctataacat aaagagagtc gggtcatcca gtgtgcgaag gccatacctt
     1021 tcaaaaatgc ccctggtaaa ttctgtagcc ttgtagaaaa aagaggattt ctcgggctac
     1081 gagcaggttt tacatcattt ggaaaaatcc gtatactcga ggcgctgggc atggtttatc
     1141 ctgcatctat aattgcaccc tctcaaccca catattctgg agaaaggcaa gaagaattgt
     1201 attccagtca atctttgtgg gctcgacttg tcattcctct gggccttttg ggcctgctgt
     1261 ctttggtcgg ttgtcctacg cgggaagagt atcatcctcc tgatctgatt tatcattatt
     1321 tggatattcc agtcgggaaa aaccctacgt caatccgagc cagtgatttt aacggcgatg
     1381 gatttacgga tttgatcacc agcaatattc agggaaattc cctgtcactg ctcttgggaa
     1441 aaggggacgg gagttttcaa gaccaaaaaa cgatttctgc ctgtcaggag ccgaggaatg
     1501 tggctgtgga tgattttaat cttgaccacg tgccggattt cgtcgtggcg tgctccgggg
     1561 ataatcatgc tcttgtgttt ttgggacaag gcgatggcac ttttttgcgt ggggcccaat
     1621 acctggttca tcgtacgcca gtgagtattg ccacaggaga ttttaatgaa gattttcttc
     1681 ctgatttagc ggtggccctc cgcaacgaca aaatccaaat cctgttgggg atggggaacg
     1741 gcaaatttcg attgggaccg ttatatgaat atggagatac gcctacctct gtggctaccg
     1801 aggatttgaa tggggataaa catttggatt tggccgtgtc caatggaggg ccgatgagtc
     1861 atgcggtgtc gatctggctg ggtttggggg atggcacctt ccaagctcca actgactatc
     1921 gtactggcaa gaggccattg ggggtctcct ttggagactt caatactgat cgagccatag
     1981 atcttctcgt aattaacggt gaaatgaaca cgattaccgt gtttttagga aacggggatg
     2041 ggacttttca agagggaagg gagtcgggcg gtgatgcggg tccaaatttt ggagtggctc
     2101 aggattttga cggcgacaaa attccggatg tggccgttgc taatattcaa tccggaagta
     2161 tttcgttgtt atttgggaag ggcgatggga cctttcatta tcctccaaga gattatccca
     2221 ctccgcatgc cccctttgca ttaaccaccc tatcgattgc aaccggtcga ggggaagaac
     2281 cgggattggc ggtcgtgaat aatgcttcaa gtaatgtctc tattttttta catcatgggt
     2341 taaatatccg gaagggccaa ttgccggcca ctccatcttg aatacctctt caaatgtgat
     2401 gttcttgaca cccgatttct gcaatgttta cagtattttt gcctttcaga aagaggcgtg
     2461 acgccggtcc gaagcgtgat tcagcctcgt gaggagaatg tgcgaacttt tggctggtgg
     2521 tttggaatgg ggtctctctt gaccttagct gtcttgcttg ttcagggagg catgagagac
     2581 ctgatctatg gttcttcgta ttctgggaca tgggaaggaa tactttccat gggactgacg
     2641 gtttttggag gtggcgttct tgcgggctgt gtcgcagtca ttttaaatcg acttcgctag
     2701 gcaacttgta acctccattc gcccacgaga gggctcttaa tcaagcgtag gtctgtatcg
     2761 ttatgacgtg cctcagatgc caaggaacca tgttagttga acgtcagtac acccgagtgt
     2821 cccgccggtt caatgcgaaa tgtatcaatt gcggtttttg gtttgactta gctgatgtgt
     2881 tgcgcttttt taagcgtgtt cttgaaagcg ggtacaacgg tcaaaaaatc cgcgaaaccc
     2941 tctagcctgt ctgaagcctg ccgccttatt ttcaactctg caactcaaaa aattaatgaa
     3001 ctgatcaagt ctctgtctgt tccttgtggt gtg
//
LOCUS       DATIAL010000380         4490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_607270, whole genome shotgun sequence.
ACCESSION   DATIAL010000380 DATIAL010000000
VERSION     DATIAL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607270"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..466
                     /locus_tag="VLA60_11780"
     CDS             176..466
                     /locus_tag="VLA60_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10087.1"
                     /translation="MYVWSKPLVLGLVLALVLLEGMFFFLIGEEAESQLHVRTTQWIT
                     ANYVLLMIWIFVWMYDQARVRGKNVWLWVLPYVFFPLPTLAFFIFRLQRRIV"
     gene            complement(473..1852)
                     /locus_tag="VLA60_11785"
     CDS             complement(473..1852)
                     /locus_tag="VLA60_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HSF10088.1"
                     /translation="MTDGFGRLLIARFVGFRLLPGQIVALAALGLILFGACLLMLPFA
                     THPGVNISFLDALFTATSAVCVTGLIVMDTPQDFTLFGQGVVLGLIQIGGLGYALMAT
                     MILLILGRRLGLRDRMMLSESMNTMDLQGLVRFVKLVAMITFGLEGLGAVLLTLRFAV
                     DLPWVTAAYYGVFHSISAFNNAGFALFSDSFKSFQTDWTINGIITILVIFGSIGFFVF
                     EDLLGNFRGQRFRLQTHTKLVLVTTALLIVGGTIGITILEWNNPATFQSASIGKKLTI
                     SYFHSVSRTAGFTSMDIVDMRDATLYFLLLLMAIGGSPGSMAGGLKTTTAAIVFLTIL
                     NMLRRDRDVEVFNRRIPQDLITRALCFTVLAIVMITGMTLLLDSTESQPFLFLMFEIT
                     SAMGIVGMSLGNGETLSLSALFTDFGKVMIMLSMLLGRFGPLMIGLFAVKTAVSKPYR
                     YAKARVIIG"
     gene            2151..3536
                     /gene="mtaB"
                     /locus_tag="VLA60_11790"
     CDS             2151..3536
                     /gene="mtaB"
                     /locus_tag="VLA60_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HSF10089.1"
                     /translation="MNSEYCQQPQPRVTLYTLGCRLNQAETALLKDGFQQSGFIPVEF
                     GQETDVFVINTCTVTEGAEVECRRIVRQVLRHSPHAFVAVTGCYAQTGLEVLRRMDDI
                     DLIVGNQFKMKLPEIIPPFPRPKKQPVPLVHHGRIDPENFSIEGVGAYTTTRANLKIQ
                     DGCQFMCSFCLIPFARGRERSRFLEDAVCEAELLVERGHRELVLTGVNIGQYRDGAAD
                     LLALIQRLEAITGLERIRISSIEPTTVPESLLDYMSSSTKLCRHLHVPLQSGDDRILQ
                     AMNRRYSVREYQEAMESALKRIPDLCFGTDVLVGFPGEGPREFANTEAVVRGFPFAYL
                     HVFSYSPRPGTASTKLPHPVSPVTTKERSRSLREMSDHKRMAFQQRFVGRRVSVLFEA
                     AEADGYWPGLTDNFLRVTVRSPHLLRNTIQPVVVTGMMSDTTLGLLEPSSESPPPAQD
                     LFPASSLVCVS"
     gene            3550..>4490
                     /locus_tag="VLA60_11795"
     CDS             3550..>4490
                     /locus_tag="VLA60_11795"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HSF10090.1"
                     /translation="MSGHPLLLPTSLKARTRSPGQSGYQVYMETFGCQMNEYDSEIVR
                     SLLKTRGFQFTPHDDEADVILFNTCAIRENAHNRVYGHLARYTNRKQERGLVIGVLGC
                     MAQNLKQELMDTRSFIDVLVGPDGYRRLPDLLIDALEHRKRGLAVDLSEYETYAHIVP
                     DRPDGINGWIAVMRGCDNFCSFCVVPYTRGRERSRDPEGIIQEAHQLVSQGVRQITLL
                     GQNVNSYRSGEWDFARLLIAVGDVPGIQRVRFMSPHPKDFPPALLEAVAEHPAICKTI
                     HLPLQSANDRILERMGRGYTQREYRKLVEAIRKKGPLV"
BASE COUNT         1095 a         1030 c         1246 g         1119 t
ORIGIN      
        1 tagaggaaaa atgtgttcag tgtgaaagac gtcaggaggg aatcgatagg acatcccttg
       61 gagaatgttt tgatttgcga cggtatctta tgccacgtta ggacctgtcg caggtggacc
      121 ggctagggtc cctagggaga ccaggaaaac tactttttaa agaaggagcc aaatcatgta
      181 tgtgtggagt aaacccttgg ttcttggatt agtgctggcg ttggtcttgt tggagggaat
      241 gtttttcttc ctgatcggcg aggaggcgga aagccagtta catgtccgaa cgactcaatg
      301 gattacggcc aattatgtgt tgctcatgat ttggatcttc gtctggatgt atgatcaagc
      361 cagggtaaga ggaaaaaatg tgtggttgtg ggtcttgccc tatgtcttct ttccgcttcc
      421 gacgctggca ttttttattt tcaggcttca gcgacggatt gtctgagtcg atttacccga
      481 taatcactcg ggccttggca taacgatagg gtttgcttac cgccgttttc acggcaaata
      541 atccgatcat taacggcccg aaccggccca atagcatgga tagcataatc atcacttttc
      601 caaagtcggt aaagagagct gataagctca gtgtttcgcc gtttcctaat gacataccca
      661 caatgcccat ggctgaggtg atttcgaaca tcagaaataa aaacggttgt gactcggtag
      721 aatccaacag gagggtcatg ccggtaatca tgacaatggc caggacggtg aagcaaaggg
      781 cacgggtaat gaggtcctgg ggtattcgcc ggttaaagac ctctacgtct ctatctcgac
      841 gaagcatatt taagatcgtc agaaatacga tagcggcagt ggtcgttttg aggcctcctg
      901 ccatactccc gggcgatcct ccgatggcca ttaataataa caggaaatag agggtggcat
      961 cgcgcatgtc gacgatatcc atggatgtga atcctgcggt acgggaaacg gagtgaaaat
     1021 aggaaattgt cagctttttc ccgatggagg cggattggaa tgtggcggga ttgttccact
     1081 ccaatatggt aatgccgatt gttccaccca cgataagcag ggcggtggtg accagcacca
     1141 atttggtatg ggtctgtaga cgaaagcgtt gcccgcgaaa attgcccaga agatcttcaa
     1201 agacaaaaaa cccgatgctt ccgaagatca ccaaaatggt gatgattcca ttgatggtcc
     1261 agtctgtttg aaaggactta aagctatcag aaaaaagggc aaacccggca ttattaaagg
     1321 ccgagatgga atggaatacc ccataatagg cggcggtcac ccagggcaaa tcgacggcaa
     1381 agcgaagcgt gagcaacacc gcgccaagcc cctctaaccc gaaagtaatc atggccacca
     1441 gcttcacgaa ccggaccaat ccctgtaaat ccatcgtatt catggattcg ctgagcatca
     1501 tgcggtctcg caatccaagg cgacgcccta aaatgagtaa gatcatcgtg gccattaagg
     1561 cgtatcccaa tcctccaatc tgaatcaatc cgagaaccac gccttgtcca aacaaggtga
     1621 aatcctgagg agtatccatg acgatgagac ccgttacgca gactgcagag gttgccgtaa
     1681 agagcgcgtc aaggaatgaa atgttcactc cgggatgagt ggcaaatgga agcatgagca
     1741 gacaggcacc gaagagaatg agcccgaggg cggctaatgc cacaatctgg cccggtagta
     1801 accgaaagcc gacaaaacga gctatcagga gtcttccgaa accgtcagtc attgtttgag
     1861 gggagagggt tcgattgaac gtgaatcagg acaggcgcag agggtcatgg tgttcgaacc
     1921 aacgggactg ccaagctgtg gtccatatcc aacaagcaca ggccttttgt tataaaaaat
     1981 agaagtgggc gggagacaag attgaaagac gatagataac tgtagggcaa tccccgaatg
     2041 tgcccggctg catctagagt ttaaaaaata ttagcatgtt cggacggcaa tggaaacctg
     2101 gtttcccctg ccttgttgtg tggaaaggcc ttctttataa tgctttgcct atgaattctg
     2161 agtattgcca acaaccccaa ccacgtgtga cgttgtatac gttaggatgt cgactcaacc
     2221 aggctgaaac ggctctactt aaagacgggt ttcagcaaag cgggtttatc ccggtcgagt
     2281 ttggccagga gacggatgtt tttgtgatca atacctgcac cgtcacggaa ggggctgagg
     2341 tggaatgtcg acgaatcgtt cgccaggtcc tccgtcattc cccccatgcc tttgtcgctg
     2401 ttaccgggtg ttatgcccag accgggttgg aagtgcttcg tcgcatggac gatattgacc
     2461 tcattgtcgg caatcaattc aaaatgaagc tgcctgagat cattccgcct tttccccggc
     2521 ctaaaaaaca acctgtgccc ttggtgcatc atggacgcat tgatcctgag aatttttcga
     2581 tagaaggcgt tggagcttat acgacgactc gcgcaaatct aaaaattcag gacgggtgtc
     2641 agtttatgtg ttcgttttgt ctgattccct ttgccagagg ccgggagcgg agtcggtttc
     2701 tggaggatgc ggtttgtgag gccgagctgt tggtggaaag ggggcatcgg gagttagtgc
     2761 tgacgggggt gaacatcggg caataccgtg atggagctgc cgatctcctg gcgctcattc
     2821 aacgccttga agcgattaca ggcttggagc ggatccgtat ttcctctatt gaacccacca
     2881 cggttcctga gagtctcctg gattatatga gtagttccac caagctgtgc cggcatttac
     2941 atgttccctt gcaaagtggg gacgatcgta tcttgcaggc tatgaatcgc cggtattccg
     3001 tgcgggagta ccaggaggcc atggaatcag cgctgaaacg aattccagac ctgtgtttcg
     3061 gcacggatgt gctggtagga tttcctgggg agggtccacg agaatttgcg aatacggaag
     3121 cggtggtcag gggttttcct tttgcgtatt tgcatgtgtt tagttactcg cctcggcccg
     3181 gtacggcttc caccaaatta ccgcatcccg tgtctcctgt tacgacgaaa gagcggagcc
     3241 gatctttgcg ggaaatgtcc gatcacaagc gaatggcgtt ccaacagcgg tttgtcgggc
     3301 gacgggtctc ggtgttattt gaagcagcgg aagccgacgg gtattggccg gggctcacgg
     3361 ataattttct tcgtgtcacg gttcgttctc cacatttgct acggaatact attcagccgg
     3421 ttgtcgtgac cggcatgatg tcggatacca cgttgggact gctggagcca tcatctgaaa
     3481 gtcctccgcc cgcacaggat ctttttcctg cgtcttcact tgtatgcgtt tcataggaag
     3541 gtgatgtttg tgagcggaca ccctttactt ctccctacca gcctgaaggc ccggacccgt
     3601 tcccctggtc aaagtggcta tcaggtgtat atggagacct ttggttgtca gatgaatgaa
     3661 tatgattcgg aaatcgttcg gtcgctgtta aaaacccgag gatttcaatt tacgccgcat
     3721 gatgatgaag ccgatgtgat cctgtttaac acctgtgcca ttcgtgagaa tgcacataat
     3781 cgcgtgtatg gccatttagc ccggtatacc aatagaaaac aagaacgtgg cttggtcatc
     3841 ggggtattgg gctgtatggc gcagaatctg aaacaggagc tgatggatac ccgatcgttc
     3901 attgatgtgc tggtcgggcc ggatgggtac cggcgactgc ccgatcttct tatcgatgcg
     3961 cttgagcatc gtaaacgagg gttggccgtt gatctgtccg aatacgaaac gtatgcccat
     4021 attgtcccgg accggccgga tggaattaat ggctggatcg ccgtcatgcg gggctgtgat
     4081 aatttttgtt ccttttgtgt ggtgccctat acgcgaggac gagaacgctc tcgggaccct
     4141 gaggggatta ttcaggaagc ccatcaactg gtcagtcagg gggttcggca aatcacgtta
     4201 ttgggacaaa atgtcaattc ctaccgttca ggggaatggg attttgcccg attgttaatc
     4261 gccgttgggg atgtcccagg gattcagcgt gtgcggttta tgtctcccca tcctaaagat
     4321 tttcctcctg ccctgcttga agccgtggcg gagcatcccg ccatctgtaa gacaattcat
     4381 ttgccgctgc aatcggctaa tgatcgaatt ctggaacgta tggggcgggg gtacactcaa
     4441 cgggagtacc gtaagctggt tgaggccatt cgaaagaaag gtcctcttgt
//
LOCUS       DATIAL010000381         8626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_393528, whole genome shotgun sequence.
ACCESSION   DATIAL010000381 DATIAL010000000
VERSION     DATIAL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393528"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..630
                     /locus_tag="VLA60_11800"
     CDS             82..630
                     /locus_tag="VLA60_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HSF10091.1"
                     /translation="MTRLPALNPESAIGQAQRLLKGVESKLGFAPNIMRTMANSPAVL
                     QGYLDFSGALSKGHLSAKFREQIALTVSEVNDCRYCLAAHSAIGRTVGLSDEAISDSR
                     RGESLDTKEATALEFTRQMLSNRGQVTDEEVAKLRKAGFTNGEVTEILANIGLTLFTN
                     YFNHVAGTEVDFPAVSGLEARV"
     gene            697..1062
                     /locus_tag="VLA60_11805"
     CDS             697..1062
                     /locus_tag="VLA60_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HSF10092.1"
                     /translation="MATQTALNLQYRLEDLFAYIREGRILDAINEFYAEDAAMQENNE
                     SPTVGRKANLEREKQFLSTVKEWKRFEVTAKGAGDDVTFYETVMDWVASDGTPVHVEQ
                     VVVAKWQGGKIIHERYYHN"
     gene            1102..1296
                     /locus_tag="VLA60_11810"
     CDS             1102..1296
                     /locus_tag="VLA60_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10093.1"
                     /translation="MALSNPGMDFIPDQTALVITDPQNIFLCPGGVAWSVVGESVTEN
                     NTVEHLKTLHSGSRVRKQFV"
     gene            1362..2411
                     /locus_tag="VLA60_11815"
     CDS             1362..2411
                     /locus_tag="VLA60_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HSF10094.1"
                     /translation="MKRFNIIFKPLIGILAVVPFGLIGMLVVGATASKSGNQDPATAQ
                     TTSVEIHYPTLHKTVNVNGLDIFYREAGPKEAPVILLLHGFPTSSHMFRNLIPQLSDR
                     YHVVAPDYPGFGNSSMPTVDQFTYSFDNLAKVMEQFTQKVGLKSYTLYLMDYGAPVGF
                     RLAVRHSERVESLIIQNGNAYEEGLREFWKPFRAYWKDRTPENARALKGFLNLPATKW
                     QYTHGVRDPEAISPDNWLVDQYLLDRAGNNDIQLQLFDSYGSNPPLYPKWQAYLREHQ
                     PPTLIVWGKNDQIFPAEGAYPYKRDLNNLEFHLLDTGHFALEEDGPVIATHIRHFLDT
                     RVVSGQSDHVKENPS"
     gene            2408..3028
                     /locus_tag="VLA60_11820"
     CDS             2408..3028
                     /locus_tag="VLA60_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSF10095.1"
                     /translation="MTQLTSDIAFTPSVKAVQERLGSRQHYTRMEEGEGWLDTITPEL
                     ADFICERDSFYFATASGAGRPYIQHRGGPKGFLRVLDARTLAFADFKGNRQYISLGNL
                     AENHQAFLFLMDYATQTRIKMWGTAEVIEDAHDLLKQLTDSQYKGKPERVIRFHVEAW
                     DANCRQYIPGKYSQEEVDRLMLPLKNRITALETEIEILNRQLRVSV"
     gene            3169..3702
                     /locus_tag="VLA60_11825"
     CDS             3169..3702
                     /locus_tag="VLA60_11825"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HSF10096.1"
                     /translation="MMNHESEQWEEGFRTLFESKVTAYKNGERRVDVMFTKQEDDFLR
                     SIGATPQEIFDFVEDWCDAGEPDPETALAITRIRRDYFLNDQQGKYSKFVKITELFPP
                     REASLAGLEWFPRIIEKARAKLYGELPPDLMYGCGADRRFLKKVNVNPVEFLQMVREA
                     EDDVDRIVKFVTERSQT"
     gene            3733..4722
                     /locus_tag="VLA60_11830"
     CDS             3733..4722
                     /locus_tag="VLA60_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSF10097.1"
                     /translation="MPKIVRFHQTGGPDVLRLEDLPLAEPGKGEVRMKVEAIGLNRAE
                     VMLRKGQYLETPQLPSRIGYEAAGVIDAVGPDITDLRIGDRVSTIPSFPMSQYGVYGE
                     SAIVPASAAAPYPDNLSAIEGAAIWMQYMTAYGALIEYGNLKTGDTVWITAASSSVGL
                     AAIQIVKATGGVAIATTRGADKKAFLLQAGADHVIVTDEEDLVERGMALTSGKGARLV
                     FDPVAGPLLEQLAAVTAPGGIIFEYGALAPHPTPFPLFPALAKGLTIRGYTLFEIVKN
                     PEMLKRGKDYIYQGLESGALKPIIDRTFPLAEIAEAHRYMVSNQQKGKIVVTV"
     gene            complement(4751..5437)
                     /locus_tag="VLA60_11835"
     CDS             complement(4751..5437)
                     /locus_tag="VLA60_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF10098.1"
                     /translation="MSSPTDPDNTSSPTTGGIIDLMTSLAMIFILLFAAFITQTPSEA
                     QSQLQEHKENVQAALRDHLERLGLTLESDPHDPLTLLIVVPDNKLTFEFGQSMLSHDA
                     VQFLQEAMPFYAAVLCGPLRDKIDSLAIQGHTDDRGDDAYNLKLSQERSLAVMVKGLE
                     AIEAQEPSAYQCFQAMTSASGRGRQDLIYDGHSRVNREKSRRVIFKIRLRSTEQRHMA
                     EGLPPILPSL"
     gene            complement(5434..6657)
                     /locus_tag="VLA60_11840"
     CDS             complement(5434..6657)
                     /locus_tag="VLA60_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10099.1"
                     /translation="MPSTFGLWEFLSRDVTFLGGPNSPMLSWLGSVGIVVFFLWHILK
                     MRKETASVRLAFNRVRPMLDGLANERKEIDRNRFTHHPGKGFSTHVDRAASSSSRLDS
                     EDIHTLDSGMQQEPIFRDAWAQYRMTLILEHVPWFVDPRIFSTKRAAEVFTQEALIAK
                     HVNLPFYRQLPSFITSLGLLLTFLALFIGLGKLHAEGSEIVGIQGLINGLAGKFLTSI
                     VGLIVANVFTFIEKPLMVRLINDHYIFLGLIDQLFPRKTMEQMLEQLTAMQGRSPKDH
                     SASGGESRPQAGWETNGLAGSSADLTAAIQTLMTLQQEQHAEIRRALSELPRVMTDRL
                     HGPLNDLNDAIHSLTKCLKEANLHPMPTETPFENRPALWKTSAYARSPSMISFFDKIS
                     SWSKRPGPVRHRRTG"
     gene            7170..>8626
                     /locus_tag="VLA60_11845"
     CDS             7170..>8626
                     /locus_tag="VLA60_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF10100.1"
                     /translation="MEALRTTTPTILVVDDEVDICLALSDLLESEGYQVETVQTGSEA
                     LRRVSPVHSYSAVILDLGLPDLDGLIVLQRLYGQDPTLPVVILTAHGDQKEKILTLQH
                     HAFSHLIKPYDRQEVVEMVHRAVAAKDLTWKAVLAEEALTSSESKRQLEQQRTQALLS
                     ESEQRLRLALKAGNMGIWDWHIPTGHLIWSEEVAGLFGLAHGMLDETFEAYENCIHPD
                     DRAVLKDIIGRTLETGAPYEVEHRIVWPTGEVRWVACKGQVVKDQDGLPQRMLGIVQD
                     ITTRKQAEIHLQDSQRFLTSIVDNLPHMIFVKDAKDLRFIRFNQAGEELIGYSKEAMI
                     GKTDYDLFPKTEADFYTRKDREVLSGKTQVDIPDEVIHTKFHGTRYLHTKKIPIFDEE
                     GNPQYLLGISEDITERKQADLGLVERNRMLELDVEVATVINQKEAIPELLQGCTEALV
                     RHLDAAFARIWCLDETKQVLNLCASSGLYTHLDGP"
BASE COUNT         2185 a         2071 c         2353 g         2017 t
ORIGIN      
        1 atcgattggc acggtcattg taataccacg aaaaaatgaa tgtgaacgtt ttccaatacc
       61 cacataagga gaacacaaac catgacacga ttacctgcac tcaatccgga gtccgccatc
      121 ggccaggccc aacgtttgtt gaaaggcgtg gaaagtaagc tcggcttcgc gcctaacatt
      181 atgcggacca tggccaactc gccggcggtc cttcaggggt atctcgattt ttcgggagcc
      241 ctctcgaaag gccatctatc ggcgaagttt cgcgaacaaa ttgccctgac cgtttccgag
      301 gtcaatgact gccggtattg tctcgcggcc cactcggcca tcggccgaac ggtggggctc
      361 agtgatgagg ctatcagtga cagccggcgc ggggaatcac tcgacaccaa ggaggccacg
      421 gccctggagt ttacgcggca gatgctgtcg aaccggggac aggttacgga cgaagaggtt
      481 gccaaactcc gcaaggccgg gtttacgaac ggggaggtca cagagatcct ggccaatatc
      541 gggctgacac tgtttaccaa ttatttcaac cacgtcgccg ggacggaggt ggacttccct
      601 gcggtttccg ggctggaagc cagggtctaa aaccgtcgag gtctaaattc agaaccacca
      661 ttactcaatc attcatttca tgaaaggaga aaaaccatgg ccacgcaaac tgcccttaac
      721 cttcaataca gactggaaga cttgtttgca tacatccggg aaggccgtat tctggatgcc
      781 atcaatgaat tctatgcgga agatgcggcg atgcaggaaa acaatgagtc cccgacggtg
      841 gggcgcaaag ccaatttgga aagggagaaa caatttctca gcacggtgaa agaatggaaa
      901 cggttcgagg tgacggccaa gggcgccggc gacgacgtga cattttatga aacggtcatg
      961 gattgggtgg cttctgacgg cacgccggtt catgttgaac aggtggtggt cgccaaatgg
     1021 cagggcggca agatcatcca tgagcggtat taccacaact aatccaaaat aattttcacc
     1081 tattcaaaaa tgagattgat catggcactt tccaacccag gaatggactt tattcctgat
     1141 cagacggccc tagtcattac cgacccccag aatatttttt tatgcccagg aggcgtggcg
     1201 tggagcgtgg tcggcgaaag cgtgaccgag aacaacactg ttgagcatct caaaacactt
     1261 cacagtggca gccgtgtaag aaaacaattt gtttgacctc gatggtctct tttggaaacg
     1321 taaaggctca ccgaatcaac agggtaaagg agaacgacat catgaaacgc tttaatatta
     1381 tttttaaacc tctgattgga atattggctg ttgtgccatt cgggcttata ggcatgttgg
     1441 ttgtgggggc gacggcctcc aagtcgggaa atcaagaccc ggccaccgct caaaccacgt
     1501 ccgtggagat ccattatccg actctccata aaacagtaaa cgtaaacgga ctggatatat
     1561 tttatcgcga ggcggggcca aaggaggcgc cggtcatttt attgcttcat gggttcccga
     1621 cttcgtctca catgttccgc aacctcattc cgcaactatc ggatcgatat catgtggtgg
     1681 ctccggatta cccgggcttt ggaaatagtt ccatgccaac cgtggatcag tttacctata
     1741 gctttgataa tcttgcgaag gtcatggagc agttcaccca aaaagtgggc ttgaaatcct
     1801 ataccctcta tttgatggat tatggagcgc cagtaggctt ccggttggcg gtcaggcatt
     1861 ccgaacgggt tgagtcgctc attatccaga atggaaacgc gtatgaggag ggcttgcggg
     1921 aattttggaa accgtttcgg gcgtattgga aagatcgtac gccggaaaat gccagggccc
     1981 tcaagggctt tctgaatctg ccggcaacca aatggcagta tacccatgga gtgagagatc
     2041 cagaagcgat tagtcccgat aattggctgg ttgaccaata tctgttggac cgggcaggca
     2101 ataacgacat tcagttgcag ttattcgata gttacggctc gaatccaccg ttgtatccca
     2161 aatggcaagc gtatctgcgt gaacatcaac cacccacgtt gattgtgtgg ggaaagaacg
     2221 atcagatttt tcctgccgaa ggcgcatatc catacaaacg ggatttaaat aacttggaat
     2281 ttcatctgct ggacacaggg cactttgccc ttgaagagga cggaccggtt attgccactc
     2341 acattcgcca ttttctggat acgcgggttg tttcgggaca atccgatcat gtgaaggaga
     2401 atccatcatg acacaactca ccagcgacat tgcatttact ccgtccgtga aagccgtgca
     2461 ggagcgactg gggtcacgac aacactatac gcgaatggaa gagggggagg gatggttgga
     2521 taccattaca ccggagcttg cggactttat ctgtgagcgg gactccttct atttcgccac
     2581 cgcgagtggg gcaggaaggc cttatattca gcatcgaggc ggccccaagg gttttctcag
     2641 ggtgctggat gcccggacac tcgccttcgc cgatttcaaa ggcaatcgcc aatatatttc
     2701 attaggaaat ctggccgaaa atcatcaggc ctttttgttc ctgatggatt acgcgaccca
     2761 aacccgaatc aagatgtggg gaactgcaga ggtcattgaa gatgctcacg acttgctcaa
     2821 acaactcacc gattcacaat acaagggaaa gcccgaacgg gttattcgtt ttcatgtgga
     2881 agcctgggat gccaattgcc gacaatacat tcccggcaaa tattcacaag aggaggtcga
     2941 ccgccttatg ttgcccctta agaaccggat cacggccctg gaaaccgaaa ttgagatatt
     3001 aaacagacaa ttgcgggtgt ccgtgtaaac gaatgtcagt cgattactgt caatggccat
     3061 gctgaaatag gagacaagga aatgaacgca tccgtacaat tcaaggaacc aatgtccgaa
     3121 ttgataggag cttctgatta tacacacaga actgcaaggg aacatcgaat gatgaatcat
     3181 gaatctgaac agtgggaaga gggattccgg accctcttcg aaagcaaggt cacggcctat
     3241 aaaaacgggg agcgccgggt ggatgtgatg tttacgaaac aagaggacga ttttcttcga
     3301 tcaatcgggg ccacgcctca ggaaattttc gactttgtgg aggactggtg cgatgccgga
     3361 gaacccgatc cggaaaccgc tctcgccatt accaggataa ggcgagacta ttttttgaac
     3421 gatcaacagg gaaaatattc gaaatttgtg aaaattaccg aattgttccc gccccgggag
     3481 gcctctctgg ccggactgga atggtttccc cgtataatcg agaaagccag agccaagctt
     3541 tatggagagt tgccacccga tctgatgtat ggttgcggtg cggatcgtcg atttctgaaa
     3601 aaggtcaacg tcaatcccgt tgaatttctc caaatggtcc gggaggcgga ggacgacgtg
     3661 gaccggatcg tgaaatttgt gacggaacga tcacaaacct gaccgaaatg atttttttga
     3721 aggaggtggc tgatgccaaa gatcgtgcgc tttcatcaaa caggcggacc tgacgttctg
     3781 aggcttgagg acttaccttt ggccgaaccg ggcaagggag aagttcgcat gaaggtggag
     3841 gcaatcgggc taaaccgcgc cgaagtcatg ctgagaaaag gccaatatct ggagactcct
     3901 caactgccct ctcggatagg gtatgaagcg gcaggagtca ttgatgccgt cggtcccgac
     3961 atcacagatc ttcggattgg ggacagggtc agtacgatcc cgtcctttcc catgagccaa
     4021 tatggtgtct atggtgagag tgccatcgtc ccggcctctg cggccgctcc atatcccgac
     4081 aacctctccg ccattgaagg tgccgccatt tggatgcaat acatgacggc gtatggggcg
     4141 ctcattgaat acggaaatct gaaaacaggc gacacggttt ggattacggc cgccagcagc
     4201 agcgtgggtt tggcggccat tcaaatcgtt aaagcgaccg gaggagtcgc gattgcgacg
     4261 actcgtggcg cagataaaaa agcctttctc cttcaagcgg gagcggatca tgtgattgtg
     4321 accgatgaag aagatctggt ggaaagaggg atggctctca cctctggaaa aggcgcgcgc
     4381 ctggtgtttg atcctgttgc gggtccgttg ttggaacaac tcgccgccgt caccgcacct
     4441 ggagggatta tctttgaata tggggcattg gctccacacc caaccccatt tcctttgttc
     4501 ccggcactgg caaaaggatt aaccatacga ggctatacgc tgtttgagat cgtcaaaaac
     4561 cctgaaatgc tgaagcgagg gaaagactat atttaccagg ggttggaatc cggcgcttta
     4621 aaaccgatca tcgatcgcac gttccccttg gccgaaattg cggaagcgca tcgctacatg
     4681 gtgtccaacc agcaaaaagg gaagatagtg gtgactgtgt gaaatttgga agtgtattac
     4741 aggttgatgc ttacaacgat ggaagaattg ggggcagccc ttcagccatg tgacgttgct
     4801 cggttgaacg gaggcggatt ttaaagatca cgcggcggct cttttcgcgg ttcaccctgc
     4861 tatgcccatc gtagatcaag tcttgtctgc cacggccgga agctgacgtc attgcttgga
     4921 agcattgata ggccgacggc tcttgagcct caatggcctc cagccccttc accatcacgg
     4981 ccagggatcg ctcctggctt aattttaaat tatacgcatc gtccccgcga tcgtcggtgt
     5041 gaccctggat tgcgagggaa tcgatcttgt cgcgtaaggg tccgcataag acggccgcgt
     5101 agaacggcat cgcctcctgt aaaaactgaa cggcgtcatg agaaagcatg ctttggccga
     5161 actcaaaagt gagtttattg tcggggacga cgatgagaag cgtgagcgga tcgtggggat
     5221 cggactccag ggtcagtcct aatcgctcca gatggtcccg aagggctgcc tgcacatttt
     5281 ctttgtgttc ttgcaattgc gattgggctt cggagggggt ttgggtgata aaggccgcaa
     5341 agagcagaat gaagatcatg gccagtgagg tcatgagatc gatgatcccg cctgtcgtgg
     5401 gagaactggt gttatccgga tccgtcgggg aactcatcct gtccgccggt gtctgactgg
     5461 gcccggccgt ttggaccagg agctgatttt atcgaaaaag ctgatcatgg aagggctccg
     5521 agcgtatgca gaggtcttcc agagggctgg gcggttttcg aaaggtgttt ctgtcggcat
     5581 gggatgcagg ttggcttcct tgaggcattt ggtgaggctg tgaatggcat cgttcaaatc
     5641 attgagggga ccatgaagcc ggtcggtcat gacgcgtgga agttccgaca gggctcgtcg
     5701 gatttcggca tgttgctcct gctgcaatgt catcaaagtt tgaatggccg ctgttaaatc
     5761 ggccgatgac ccggcaagcc cgtttgtctc ccatccggcc tgtgggcggc tttctccgcc
     5821 ggaggccgag tgatcttttg gggatcggcc ttgcatggcc gtgagttgct ctaacatttg
     5881 ctccatggtt tttcgaggaa agagttgatc aatgagacca agaaaaatgt aatgatcgtt
     5941 tatcagtcgg accatgagcg gtttttcaat gaacgtgaag acattggcca caataagtcc
     6001 gacgatggat gtcaggaatt tgcctgccag cccgtttatc agcccctgaa ttccgacaat
     6061 ctcacttccc tcggcatgta gcttgccaag gccgataaac aaggcgagaa aggtcagcag
     6121 taacccgaga ctcgtgatga acgaaggcaa ctgtcgatag aacggcagat tcacatgttt
     6181 ggcaatgagg gcttcttgag taaacacctc tgcggcgcgt tttgtgctga atatccgtgg
     6241 gtccacaaac cacgggacgt gctcaagaat gagtgtcatt cggtattgcg cccatgcatc
     6301 gcgaaagatg ggttcctgtt gcattcccga gtcgagcgtg tggatgtctt cactgtccag
     6361 ccggctcgaa gaggatgctg cccggtccac atgggttgag aacccctttc cagggtgatg
     6421 cgtgaagcgg ttgcgatcga tttcctttcg ttcattggcc agtccatcca acatgggccg
     6481 tactcgattg aatgccagac gaaccgaagc cgtttccttt cgcatcttga gaatgtgcca
     6541 cagaaaaaat accacaatcc caacagaccc cagccaactc agcattgggc tgttcgggcc
     6601 tcccaggaag gtcacgtcac ggctcaaaaa ttcccacagg ccaaacgtag agggcatctt
     6661 gtcctcagcc tttcgctaat aattgcctac ggcttatgca tggcagagtt gttgatcgtc
     6721 aaattcgtat acagcattct caagtttcgt gccgtcacta aaacaagtag aaactcataa
     6781 aataagggaa gttttttgaa tgtttgggaa ccttgggaga agcgttgggc aaagagtgcc
     6841 tcctgcatcg gcaagttgat cctcaaaaaa aacgcatcca caatcatggt tccccataaa
     6901 tcctgacaca atccttctcc ctctggtccc agactgtcat cctcatccgg tgccaaagat
     6961 tgccagaccg ttggttttgt tgatgttcaa ggggttcact catgttcatg gttagggaaa
     7021 cctacaagga aaattttctt ttaaatgcgt gatagagcca atgggtttga ccctttattc
     7081 aaatggcgta caatacctcc acgattccag tcaagcttct cggctgaagg acattttggc
     7141 gaaacatagg ggaaagaaaa ggaagaaaga tggaggctct gagaaccacc acacccacta
     7201 tccttgttgt ggatgacgag gtggacattt gcttggcctt gagtgatctg ctggaaagtg
     7261 aagggtatca ggttgagacc gtgcagaccg gaagcgaagc gttacgtcga gtgtcccccg
     7321 ttcattccta tagtgccgtc atcctcgatt taggtctgcc tgaccttgat gggctgattg
     7381 tccttcaacg gctgtatggg caggatccca cccttcctgt ggtcattctt acggcgcatg
     7441 gcgatcaaaa ggaaaaaatc ctcactctgc aacatcatgc attttcccat ttgatcaaac
     7501 cctatgaccg gcaagaggtg gttgagatgg tgcatcgcgc ggttgcggcc aaggatttga
     7561 cttggaaggc cgtgctggcg gaggaggccc tcacctccag tgaatcgaaa cgtcagctcg
     7621 aacagcaacg cacgcaagca ttgctttccg aaagtgagca acgactaagg ttggccttga
     7681 aggcgggaaa tatggggatt tgggactggc atattccaac cgggcatcta atctggtcgg
     7741 aggaagttgc cgggctcttc gggttagcgc acgggatgct tgatgaaacc tttgaggctt
     7801 acgaaaactg cattcatcct gatgatcgtg cggtgctaaa agacatcatt gggagaacgc
     7861 tggagaccgg ggcgccttac gaagtcgagc atcgaatcgt gtggcccacc ggggaggtcc
     7921 ggtgggttgc ctgcaagggg caggttgtta aagatcagga tggtctcccc caacgaatgc
     7981 tcggcatcgt tcaagatatc accacccgaa aacaggcaga gatccatttg caggacagcc
     8041 aacggttttt gacctccatt gtggataatc ttccgcatat gattttcgtt aaagatgcca
     8101 aagatctccg ctttatccgc ttcaatcaag ccggggaaga gctcattggc tattccaagg
     8161 aagcgatgat cgggaagacc gattacgatt tgttccccaa aacggaagca gacttttata
     8221 ccaggaaaga tcgagaagtc ttgtccggta aaacccaggt tgatatcccg gacgaagtga
     8281 ttcacaccaa atttcacggg acacgatacc tgcataccaa aaaaatcccc atcttcgatg
     8341 aagagggaaa ccctcaatat cttttgggaa tttcagagga tattacagaa cggaaacagg
     8401 ccgatttggg attggttgag cgaaaccgca tgctagagtt agacgttgag gtggcaaccg
     8461 tcatcaatca gaaggaagcc atcccagagc ttcttcaggg gtgcactgaa gctctggtac
     8521 ggcatcttga tgcagcgttt gcgcgtattt ggtgcctgga tgaaacaaaa caggtactaa
     8581 atttgtgcgc cagctctggg ctttataccc atctagacgg accgca
//
LOCUS       DATIAL010000382         8939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_70198, whole genome shotgun sequence.
ACCESSION   DATIAL010000382 DATIAL010000000
VERSION     DATIAL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70198"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            666..845
                     /locus_tag="VLA60_11850"
     CDS             666..845
                     /locus_tag="VLA60_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004153593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HSF10101.1"
                     /translation="MTILKWAAIFFVIALIAGGFGFSGIAEGAAGIAQVLFYIFLVLC
                     VVMVLIGVFVAKKIT"
     gene            964..1257
                     /locus_tag="VLA60_11855"
     CDS             964..1257
                     /locus_tag="VLA60_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10102.1"
                     /translation="MTNQEKAKLIAPWINPAERVTVDFNDVTGLNAEVFGCTENVVYL
                     LFQEAFPHMKEQVTIPLKAVQVEEDDGHYTRDPDAPLQWRLRLRVNQNRPEGI"
     gene            1450..2208
                     /locus_tag="VLA60_11860"
     CDS             1450..2208
                     /locus_tag="VLA60_11860"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF10103.1"
                     /translation="MKFIAKERMKVGTAVMALCLGIPVLAQADSSYDYRDRSHVDTRG
                     WSDSRYYDADRDRNNDRRFYTDQYGDLDTMENQDNNHRYRGEGYVTQDGYRSTASSGN
                     LQYPNDLRRADQTIRRDILAELGEGTERVQVSVENGVATLSGMVLNREAMVEAEEAAY
                     DGGARKVRNELQIFRYDERPWGEMSDRSLAKAVRKELAWSPYVDEDPIRVDARRGVVT
                     LQGTVKDRSQMAAAVENAYEAGARRVNNQLRIAN"
     gene            3347..3742
                     /locus_tag="VLA60_11865"
     CDS             3347..3742
                     /locus_tag="VLA60_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10104.1"
                     /translation="MAPLAVMGILLVIDPDPDSQESWVRLGDSCGVEIMVAGNLSDGI
                     SLSTQQSVDLIVVDLFLPQKSGFSFIAEITSKEHHPPIIATFSAEQAPKFNIKRFAHL
                     LGASYTFEKPLNPKLFLQACRELVTHFPG"
     gene            complement(3860..4780)
                     /locus_tag="VLA60_11870"
     CDS             complement(3860..4780)
                     /locus_tag="VLA60_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10105.1"
                     /translation="MKTLSPSTNCVINNPEEFKQFIPANFSYFLERSSGLIRRILQDM
                     GQWADNSAHEKLLLRLSFDLVERFIEYCPSQLPCRPSLLLDGFISDFFGRPQEPQASS
                     TSNPVLYRFLDGLLNRAVLSRDALICLFYHFYGMKPVEVAFLLGLEEGQTQRIYKNFA
                     RWRQKGWFQAAEEVGLTTQEVQSLIENQASNPDLFHLQVRENLETLLPFYRKSDPPYY
                     PCLEGTKWQEMFREGYGFDYRMWHLPLCLSCMKVITEVGDSFLLNPEVTLNFHISPHS
                     LREEEEFWPALKNFSADKKHHNINRSLQMA"
     gene            5143..>8939
                     /locus_tag="VLA60_11875"
     CDS             5143..>8939
                     /locus_tag="VLA60_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10106.1"
                     /translation="MTSSRRMAETIRNKNWLETPLGDMAGWPSQLKTSVQIVLDSKYP
                     MFVWWGNHLINIYNDAYIPILGNRHPQALGACASDIWSDIWDVIGPQADLVFQEGRST
                     WNEDLPLFMRRHGFLEETFFTFSYSPIKNEQGDILGLFCACTEETSQVLKGRRLHTLR
                     RLSEVTEKADSVGEVCRLSAEILTDNSFDIPFSLIYLWDKDERFLCLAAQSGFQSDSL
                     LTPPRLAMDGAMIHPQPWWPLSLSPKRDETVFTINRQLNLSGGPWPEAPGQGMVLPVE
                     SWETQRQSGYLIVGLSSRLALDDRYREFLNLLAKGISGAINRAQVRAEERKRIEALAE
                     LNRAKTEFFSNVSHEFRTPLTLILGSLEEAMQTTTFTTAPGVEVAHRNAVRMLKLVNT
                     LLDFSSLEAGRMQVVFEPVDLCELTIDLASSFESAMKKAGLEFIVSCSTLSQPVYVDR
                     TSWEKIILNLLSNALKFTLKGRVSVDVYPHAESVKVRIEDSGVGMSRETLSHVFDRFF
                     RAKEALGRTFEGTGIGLALVKELVEMHGGKIHVESEPGNGSVFTVTVPFGFHHLPPEQ
                     VCHTSAQPKDERLAKSFILESQQWAGDTDHEPASQEILQDKEISLQKIDHSRPTILLV
                     EDNRDMRNYLTRLLCADYEVVSVCDGQEALDRIATVRPHLILSDIMMPRIGGNELLTH
                     LRSNHELQSTPFIFLSARAGQEAEIDGLLTGADDYLIKPFSGRELLARIKNTLGLATM
                     RHAAIEYESRFQHLAHHPEIMTWVTEADGNCVFLSRTWYDFTGQTQATGLGEGWLSAI
                     HPHDRPSIEQAFYEANRKRQSVRMEYRLRSAKGEYHYVLDSASPRFASDGGYLGYVGL
                     VLDLSCQKDIEEQHRESEERFRILANSISQFAWMADSTGWIFWYNARWYEYTGTTLRD
                     MEGWGWTKVHHPDHVNRVVEKVSRCFQTGHIWEDTFPLKGKEGEYRWFLSRAVPIRNR
                     HGEVVRWIGTNTDITELQTTQEQLQHLTNKLESQIAIRTEELRHNQSRLQALVGELIL
                     TEQRERRRIAEELHDFLAQLLVACRLRVNRLEQMVGAEGHMLPVLHEIDRILDQSLGY
                     TRSLVAQLVPSVLYQFGLLKALQWLAEDMKQYGLTVEVNMERDHLTFTENEAVLLFQS
                     VRELMLNVKKHAAVDTVYLAVRLSSSDEVCIEILDRGVGFDPNLIEKHSPTFNQFGLF
                     SIRERMSLLGGRMVIDSSSGSGTRIRLYMALSEQDPSNATLSIADSGMMVTKETSQGE
                     RSK"
BASE COUNT         2301 a         2027 c         2364 g         2247 t
ORIGIN      
        1 aatggcatat atgggtgtcc attatcgatg tcctcactat gtactatcat catctctatc
       61 aaagttcagg atgctcctac atggataaaa cctcgcggac ccttcgtaac ttgtctattt
      121 gaatgaacaa cctcatgcgc catcatgagg aacaggaaaa aaatagggtg taagaatttt
      181 tcccttcaaa gatttccatt gaaggcatgc cctctttaaa ggaaggagag aaggtgtcgt
      241 aggtggcatt caatattgta aaagttgcgg atggaggact ggatgatcgg gacatgggag
      301 gtcaatttca ctcaaggagg tagccattct ttccagggag ccaaaaaacg cccacccgtg
      361 aaacctccgc ctgaacctga gagaaaatct ccgcccatcg gtcctccgaa acgggatccg
      421 aaaccggagc cgattcacga tccgcctatg cctccatcgc cgggaaacga tcccaacgat
      481 gaacctctcc cgattggtga cccgcctgat aggtcggatc agcctattca tatgagaatc
      541 acggcaggtg gcctctccgt tggatggccc ttggactact cccgaaacag ggagggattc
      601 actttcattt atacacgtga tcccatgatc cgtgatcctc ttcaacctcg tgaaaggagg
      661 tctctatgac gatattgaaa tgggccgcca tcttctttgt cattgcgctt atcgcgggcg
      721 ggtttggttt ctcaggaatt gcggagggag cggccggaat tgcccaggta ttattttata
      781 tttttctcgt gctgtgcgtg gttatggtcc tcatcggagt gtttgtggcc aaaaaaatta
      841 cataataccc ccctgggggg aaatcattag catgaccggt caacagctct gtggcacagg
      901 ctggcgggag gcgcgaacag gagaaattat ttttcctgat aaagagctga ttaagaggat
      961 gttatgacga atcaagaaaa ggccaaattg attgcgccgt ggatcaatcc tgcagaacgg
     1021 gtcacggtgg attttaacga tgtgacgggt ttgaatgctg aagtgttcgg atgcacggaa
     1081 aacgtggtct atttgttatt tcaagaagcg tttccacata tgaaggaaca agtcactatt
     1141 ccgttgaaag cggtgcaggt ggaagaggat gatggacatt acacccggga tcccgatgcc
     1201 cctcttcaat ggcgattgcg cctgcgcgta aatcaaaatc gtccggaagg aatctgacat
     1261 cgctcacgga gtgggtttaa ctctatttga tccattggat atgtcgctta ggaagtacgt
     1321 aattgggagg aaggattaga cgatgggcgg acatgatccc cacaacttgt ccattcacaa
     1381 tttgccgggt tccagctatc gtttaagtaa gatcttccca acaattctat ttaacgaaag
     1441 gagagaacga tgaaattcat agcaaaggaa cgcatgaaag taggaactgc cgtcatggcc
     1501 ttgtgcttgg gcatacctgt cctggcgcag gcggattcat cctatgatta cagggaccga
     1561 tcgcatgttg atacacgcgg gtggtcggat tcccggtatt atgatgccga tcgcgatcgg
     1621 aataatgacc ggcggtttta cacggaccaa tatggtgatc tggataccat ggaaaatcag
     1681 gacaataacc acaggtatcg gggagaggga tatgtgactc aggacggata ccgctccact
     1741 gcttcctcag ggaatctaca gtacccgaac gaccttcggc gagctgacca aaccattcgg
     1801 cgagatatcc ttgccgagct gggcgagggc accgaaagag ttcaagtcag tgtagaaaat
     1861 ggcgtagcga cgctgtcggg catggtttta aatcgggaag ccatggtgga agcggaggag
     1921 gcggcctatg atggtggcgc gcgaaaagtg cgaaacgaat tgcaaatatt ccgatacgat
     1981 gagcgaccgt ggggagagat gagtgatagg agtttggcaa aggctgtgag gaaagaatta
     2041 gcatggagtc catatgtgga tgaggatcca attcgggtgg atgcccggcg aggagtggtg
     2101 acgctgcaag gaaccgtgaa ggatagatct caaatggcgg ctgcagtcga aaatgcctat
     2161 gaagccggag cccggcgtgt caataatcaa ctcaggattg ccaactaacc ggggtcccgc
     2221 aagatctgat ggtccccgaa atcgaatggt agagctgggc ttctcgtgtg cggtatagag
     2281 atattctctt aagaccgtca ggaagtagga gcagaaggaa atgacgcgat acatagccgg
     2341 tacgcaatag ttcaacagaa aggggagagt tatgccaatt ttattgtgga ttttaggagt
     2401 tcctctcagc ctcgtgctga tattatggtt gtttggagtg atcggttttt aataaaccgt
     2461 tttcaagact aaatttttgg taaaagaatt tgaacaacca agtgtcttga gtacaaatta
     2521 atatattgtc tgtaggatat gacgggcccg ggtatttcag ttgaaggaat gtccgggcct
     2581 gttactataa tgaaccaccc tctttttttg gacccggatt agcggattac cttgggtatc
     2641 cgggcacttt ttcgcgacag gctttacaaa aaactgaagc caaatgtcca tttgttcatg
     2701 tgtcgttata tggtaaagct gaatctgatc aggaagatga ctgattccta cgaatacgga
     2761 gaaagatggg gtcgacgccg atgtcagcag tgacaatcca acaggctgaa tatagagaag
     2821 ggcgtggaaa gcttgttttt tttaatgtag tagtgggagt attggttaca gctctcctgt
     2881 gtgcttctca agtaatccct cttgctgaag gcttccccac acattcgatt caaggtcatt
     2941 ggcttcagaa gaaccgtcag gagaattcta tttctaggga ggctccaggg aggaccttta
     3001 tttattcatt ggaaccctgg ggtgggtttc gggttaacaa tgctgaaaga cctctcttcg
     3061 caatgtggga tgtctctatc ctgccttcga ggtcgggagg caatctcctg tcaaccaccg
     3121 cacaaaagcg catagttgct gaatcaaaaa cgggcatgaa gcgaggcgac attcgtccgt
     3181 ccattcaatt tttccgagta cgaaatctga attggatcac gacatcatga tgaatggcat
     3241 agctttcatg ttcaccatat aagatttgac tgaacggaag acagaagggg gatcgggttc
     3301 tagcgatgtg ggtttcttat ccggataatt cgaagggcaa aggtgcttgg ctccattggc
     3361 tgtaatgggt attcttcttg ttattgaccc cgatcctgat tcccaagagt cctgggtgcg
     3421 tctcggtgat tcgtgcggag tcgagattat ggttgcagga aatctttctg atggaatcag
     3481 tctctcgaca caacaatccg tcgacctcat cgtcgtagac ttgtttctcc ctcaaaaaag
     3541 cgggttttcg ttcattgcgg aaatcacgtc gaaagaacac catcctccaa tcattgcaac
     3601 cttctcagcc gagcaagcgc caaagtttaa tattaaacgg tttgctcatt tgctaggagc
     3661 gtcttatacc tttgaaaaac ctctcaaccc caaattattt cttcaagcct gtagggagtt
     3721 ggtgacacat tttcccggct aacccgtaaa gatatactgc ccggacagga gacagtgttt
     3781 gaacagcact gttctctcct gatgaacatt cgatcctacg gtttttagcc aaatattttc
     3841 cgtaccttga gagtgcaggt taggccatct gaaggcttcg attgatatta tgatgttttt
     3901 tatccgcaga gaaatttttc agggccggcc aaaactcctc ttcctccctc aacgaatgag
     3961 gagagatatg aaaattcaac gtcacctcgg ggttgagaag gaatgagtcg ccaacttccg
     4021 taatgacctt catgcaggat aggcagagag gtaaatgcca catccgataa tcaaatccat
     4081 agccctctct aaacatctcc tgccatttcg taccctcaag gcatgggtag taggggggat
     4141 cgctttttcg ataaaagggc aataaggttt ccagattttc cctcacctga agatgaaata
     4201 aatccggatt agaagcctga ttttcaatca acgattggac ctcctgggtg gttaatccaa
     4261 cttcttctgc cgcttggaac caaccttttt gccgccatcg tgcaaaattt ttgtagatgc
     4321 gctgggtctg cccttcttcc aatccaagaa ggaaggcgac ctcgaccggc ttcatgccgt
     4381 aaaagtggta aaacagacag attaaggcat cccggcttaa gacggctcgg ttgagcaggc
     4441 catccagaaa ccggtacaac acagggttag aggttgatga tgcttgaggt tcctgaggtc
     4501 ttccaaaaaa atctgagatg aatccatcca ataacaggga aggccgacag gggagctggg
     4561 aaggacagta ttcgataaac cgttccacca gatcaaacga aagacgcaac aataactttt
     4621 catgggccga attgtcggcc cattgcccca tgtcttgcag aatacgccga atcaggccag
     4681 aagaacgttc aaggaaatag gagaagtttg ccggaatgaa ctgtttgaat tcttcagggt
     4741 tattaatcac acagttggtt gatggggata acgtcttcaa ggtcgtcctc cttgcttggg
     4801 gaaatgaaaa tgaatgaaaa atgaaaaaat ttcataaggc catcagactg tcttgttgta
     4861 caaatttatc gagatggccc cccttcctgt actgttaaaa tcccttgatt ctcccgatac
     4921 cgaccctagg gatgccaaga ctgaaccgcg gacgttccac aggaggaaac aaaggaatca
     4981 gaggacactc tccccaaaga gaacgaaaat ccctgatttg tcaactgaaa atgcggggga
     5041 ggtgtgataa ggttgagcct gccaacccag agggattaaa ttgggtttcc ccttacttca
     5101 ttgccagcct cacaggctca accaggtgaa aggactttgt ccatgacatc gtcacgccgg
     5161 atggcagaaa ccattcgaaa taaaaattgg ctggaaaccc cactcgggga catggccgga
     5221 tggccttctc agctcaaaac ttctgtgcag attgtcctcg attcaaaata tcccatgttt
     5281 gtctggtggg ggaaccatct gatcaatatt tataacgatg cctatatccc gatattggga
     5341 aaccgacatc cgcaggcgtt gggtgcctgt gcatcggata tctggtctga tatctgggat
     5401 gtgattggtc cgcaggcgga cctcgttttt caagaaggtc gttccacatg gaatgaggat
     5461 cttcctttat ttatgcgtag acatggattc ttagaggaaa ccttttttac attttcctac
     5521 agcccgatta agaatgaaca aggagatatc ctaggactct tttgtgcctg taccgaagaa
     5581 accagccagg tattaaaagg gcgaaggctt catactctca ggcgactgtc cgaagtgacg
     5641 gaaaaagccg attctgtggg agaggtttgt cgtctctcgg ctgaaattct cacagacaat
     5701 tcctttgata ttccgttcag tcttatatat ttgtgggata aggacgaacg ctttctgtgt
     5761 ctggcagcac aatcaggttt tcaatctgat tcactcctga ctccacctcg actcgcgatg
     5821 gatggagcca tgatccatcc tcagccatgg tggccgctgt cactttctcc caagcgggat
     5881 gagacggtat tcacgattaa caggcagttg aatctttctg gtgggccgtg gccggaggcg
     5941 cccggtcagg gaatggtgct gcctgtggag tcgtgggaaa cacaaagaca atccggctat
     6001 ctgatcgtgg ggctcagttc gaggctggca cttgatgacc ggtaccgtga gtttttaaat
     6061 ctgctcgcga aggggatttc gggggccatc aatcgtgcgc aggtccgggc cgaagagcgg
     6121 aaacggattg aagcgttagc cgaactgaac cgggcaaaaa ccgaattttt cagcaatgtg
     6181 agtcacgagt tccgcacacc tctcacactt attctcgggt ctctggaaga agcgatgcag
     6241 accaccactt tcaccacggc tccgggtgtg gaggtggctc accgaaatgc cgtgcggatg
     6301 ctgaagctgg tgaacacgct gttggatttt tccagcctgg aagcgggccg catgcaggtt
     6361 gtgtttgaac ccgtggatct ctgtgagtta accattgacc tggcatcgtc gtttgaatct
     6421 gccatgaaaa aggccggtct ggagtttatc gtgtcctgtt ccacgctgtc tcaaccggtc
     6481 tatgtggatc ggacctcctg ggaaaaaatc attctgaatc tgctttcaaa tgccttgaaa
     6541 ttcacgttga aaggacgggt gtccgtggat gtctatcctc atgcggagag cgtgaaggtc
     6601 cggattgagg attcgggagt cggtatgagc cgggagacgc tttcgcatgt gtttgaccga
     6661 ttctttagag ccaaggaagc cttgggacga acatttgaag gaactggaat cggtttggct
     6721 ttagtaaaag agctggtaga aatgcatggc gggaagattc atgtggagag tgaacctgga
     6781 aacggaagtg tgtttaccgt gacggtgccg ttcgggtttc atcacctccc gccggagcag
     6841 gtatgccaca cctcggcgca acccaaggac gaacgtttgg cgaagtcgtt cattttggag
     6901 tcccagcagt gggcgggaga cacggatcat gaaccggcca gtcaggaaat tctccaggat
     6961 aaagaaattt cacttcagaa aatcgatcac tcacgtccca ccattttact ggtggaagac
     7021 aatcgggata tgcggaatta tttgacgcga ttactgtgtg ccgactatga ggtggtttct
     7081 gtctgtgatg ggcaggaggc cttggaccgg attgccacgg tccgccctca ccttattctg
     7141 agtgatatca tgatgccccg cattggtgga aatgaattgc tgacccatct tcggtccaat
     7201 catgaattgc agtcgacgcc gtttatcttc ctatcggcca gggccggtca ggaggcggaa
     7261 atagatgggc ttctgaccgg tgcggatgac tatctcatta agccgttttc tgggagagag
     7321 cttctggcca gaattaaaaa cacactggga ttggccacga tgcgtcatgc agcgatcgaa
     7381 tatgaatccc gcttccagca tctggcccac caccccgaga tcatgacctg ggttacggaa
     7441 gcagacggga attgtgtctt tctcagtcga acctggtatg actttaccgg gcaaacacag
     7501 gcaaccgggc tcggtgaggg atggttgtct gccattcatc ctcatgatcg tccatccatt
     7561 gaacaggcgt tttacgaagc gaaccgaaaa agacagtctg ttcgaatgga ataccgcctt
     7621 cgtagtgcga agggtgagta tcactatgtt ctggattccg cctctccccg ctttgcatcg
     7681 gacggcggat atctggggta tgtgggtttg gtgctggacc tttcctgtca gaaagatatc
     7741 gaggaacaac accgggaaag tgaagagcga tttcgtattc ttgccaatag catttcccaa
     7801 tttgcctgga tggccgattc gaccggatgg atcttttggt acaatgcacg ctggtatgaa
     7861 tataccggta ccacgcttag ggacatggaa gggtggggct ggacaaaagt ccatcaccct
     7921 gatcatgtga acagggtggt tgaaaaggtc agccggtgtt ttcaaaccgg gcacatatgg
     7981 gaagacacct ttcctctcaa gggcaaagag ggcgaatacc ggtggtttct ctcaagggcc
     8041 gtgcccattc ggaatcggca tggggaggtc gtgcggtgga tcggcaccaa tacggacatt
     8101 accgaattac aaaccacaca ggagcaactc caacacctga cgaataaatt agaaagccaa
     8161 attgccatac gcacagagga actgcgtcat aatcaatcgc gcctgcaggc actcgtcggc
     8221 gaactgattc tgactgaaca gcgggaacga cgcaggattg ccgaggaact tcatgatttt
     8281 ctggcccaac tcctcgtggc ctgccggttg cgagtcaaca gacttgagca gatggtgggt
     8341 gccgagggac atatgcttcc ggttcttcat gaaattgatc gcattctcga ccaatcgttg
     8401 ggatatacgc gctcgctggt ggcccaactg gtcccgtcgg tgttgtatca attcgggtta
     8461 ctcaaggcac tgcagtggtt ggcggaggat atgaaacaat atggactcac ggtggaagtc
     8521 aatatggagc gcgaccatct gacatttacc gagaatgagg cggttctttt gtttcaatcc
     8581 gttcgtgaac tcatgctgaa cgtaaagaag catgccgccg tggacacggt ctatctggcc
     8641 gtccgccttt cctcatcgga tgaggtctgt atcgaaattt tggatcgtgg cgttggcttc
     8701 gatcccaacc tcatcgaaaa acacagccct acgttcaatc aattcggatt gttcagtatt
     8761 cgagaacgca tgtctttatt aggcggacgg atggtcatcg actcgagttc cggttccggg
     8821 acacgcatcc gcttgtatat ggccttatct gaacaggacc catcaaatgc gaccttatct
     8881 attgcagatt ctggaatgat ggtcaccaaa gaaaccagtc agggagaacg ctcgaaaat
//
LOCUS       DATIAL010000383         6806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_394276, whole genome shotgun sequence.
ACCESSION   DATIAL010000383 DATIAL010000000
VERSION     DATIAL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394276"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1824
                     /locus_tag="VLA60_11880"
     CDS             <1..1824
                     /locus_tag="VLA60_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HSF10107.1"
                     /translation="HRAWASLVNRSLNMFTLIALGTGTAYVYSTVATLAPQLFPASFR
                     GKGGEVAVYFEAAAVITVLVLLGQVLELRARSQTGSAIKALLGLAPKTARRIRENGEE
                     EDVAVDSVHPGDKLRIRPGEKIPIDGVILEGQSAVDESMITGESIPVEKEADAQVTGG
                     TVNGTGTLVMRVERVGRDTVLAQIVRMVSEAQRSRAPIQRMADLVAGYFVPIVVGVAL
                     LTFLIWAMFGPEPRMAHALVNAVAVLIIACPCALGLATPMSIMVGTGRGATAGVLMKN
                     AEALEQLEKVDTLVVDKTGTLTEGKPKLMSVVSLFDMPEEDLLRLVASLERNSEHPLA
                     SAIVNGAKDKGLELVKAQDFQSKTGKGVTGTVQGHSMALGTRKFLEEEYHVRPETFGP
                     LSDKAEDLRRDGQTVMFVAIANQIAGLIGVADPIKPSTSEAIRLLHEEGIHVVMVSGD
                     HQQTAQAVAKKLGIDEVKAEVLPEEKSRIIQQLQAKGKIVAMAGDGINDAPALAQAQV
                     GIAMGTGTDVAMESAGITLVKGDLRGIARARRLSQATMRNIRQNLFFAFFYNAIGVPI
                     AAGILYPAFGILLSPMIAAAAMTFSSVSVITNALRLRTTTL"
     gene            complement(2696..6232)
                     /locus_tag="VLA60_11885"
     CDS             complement(2696..6232)
                     /locus_tag="VLA60_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF10108.1"
                     /translation="MSVEPFGVLEHLKVLSPNHIYFLASKAFVLRGYEYYAQGRLESY
                     SWDRTQSILSATVRGATQYLVRFGVQHGQLTFSCTCPVWTPESHCKHVICALLTTVNL
                     LLPDTFRMPSSNSTQRALLLRQLMERAGSPLASKPSTPTAPPRFEITLCNHHGVSSIR
                     ITNHGNICQSFAGMPTELAVLLRATQDPAWSVQEGLRDFLKHHGQHHALFYENADGRF
                     PLEWAPDALYTTKTELDILGSHVSIAACCLRYGEVQPNTYACLGFAADLNAKKLGPLE
                     HEGGWAVYDLLYENSQREQVIEPADPLSRPLALPATSHHREGWLSAERPAGMARPTFS
                     IPVEQFITLQLDIPLHEQDRILRSVLLKCAGQEVALTDHRHSGKAPTYQYRLTFIPEA
                     IPLDASVLDLRTGVLRAECWLGESQGRPSVPIFRIFPFLERAKTVSSGLKAKKRRALL
                     YDTFFQLIGHQKPTDVRKLVKACIAQDEFRPFKLKAEAEQILQRFSSPALEPSVRLVV
                     HDGHWCVVMNDWAKEALLYAIPYTLWGPSLFEGMVEHDEMSLPLTNLYPLLPELHAKL
                     QEVGIGLFYQDQPITTSQWDCSIDARRASSIDWFELRPELLCDGVRIEAKDIEKILER
                     GGMMEADGIIRIVDQITQAILRAFAFLSNKPSMEHREQKAVVRVPKLQILDWVALRKR
                     GVTVILPPADEALINRLLHFEQIEPIPLPQVVDATVRPYQQEGYQWLGFLYQHRLGAC
                     LADDMGLGKTLQAICLFAGIKEGLITAPEPIQGPHLVVLPPSLLFNWEHEISRFYPSL
                     TVQSYTGKERTPSFAGADVVLTTYGLIRRDIAILEQIPFNVILFDEAQAVKNIVARTT
                     GAARRLKGYFKCVMTGTPLENHVGEYYALMDLCVPGLLGDYEEIRGKMKAPSPALLET
                     IMQRSRPFVLRRTKSQILKELPPKIETDLYLELTDRQKSLYQQTVSKIRSTIAAAYRS
                     QTPAQARIIALTAILKLRQICLSPQLLSPAHTELSPKLECLLDRLKELLDEGHSALVF
                     SQFTSFLDIVEQAFDAHEIPHVRLDGSTPTPTRKKLVQKFQEGENPSIFLLSLKAGGQ
                     GLNLTKASYVFHLDPWWNPAVENQASDRAHRIGQTQNVSIMRLLMRHTIEEKMMELKQ
                     KKLDLYHAVLEGATPHGGGASLSQKDFDFLLE"
     gene            complement(6603..>6806)
                     /locus_tag="VLA60_11890"
     CDS             complement(6603..>6806)
                     /locus_tag="VLA60_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10109.1"
                     /translation="SRSLNQPSCLSACTSESRTLIRFGGKFFFYGADDVTDESWRGRV
                     GMLLGLALIFQHFPLSVGFSLTY"
BASE COUNT         1604 a         1817 c         1860 g         1525 t
ORIGIN      
        1 caccgcgcgt gggcttcgct cgtgaaccgc agtctcaaca tgtttacgct gattgcattg
       61 ggaaccggca cagcctatgt ctatagcacc gtggccacgc ttgcgcctca attatttcct
      121 gcctccttca gaggaaaggg cggtgaggtc gccgtgtatt ttgaggctgc cgccgtgatc
      181 accgtactcg tgctattggg ccaggtcctg gaattacggg ctcgcagcca gacaggcagc
      241 gccattaaag ccttgcttgg cctggcccca aaaactgccc ggcgaattcg cgagaatggc
      301 gaggaagaag acgtcgccgt ggattccgtg catccaggag acaagcttcg tatccggccg
      361 ggagaaaaaa ttccaatcga cggagtcatc cttgagggtc aaagtgccgt ggatgaatcg
      421 atgattaccg gagaatcgat cccggtggaa aaagaggcag atgctcaggt caccggggga
      481 accgtcaatg gcacggggac gttggtgatg cgagttgaac gagtgggtcg ggataccgtc
      541 ctggcgcaaa ttgttcgcat ggtgagcgaa gcccaacgca gccgggctcc cattcaacgg
      601 atggccgatc tggtagccgg ctattttgtc ccgattgtcg tcggggtggc gttactgacc
      661 ttcttgatct gggcgatgtt cggccccgag cctcgtatgg ctcacgccct cgtcaatgcg
      721 gtggcggtcc tcattattgc ctgtccctgc gcgttgggtt tggccactcc tatgtcgatt
      781 atggtgggta ccggacgagg agccacagcc ggcgttctaa tgaagaatgc tgaagccctg
      841 gagcaattgg agaaagtgga tactctcgtc gtcgataaaa cgggaaccct cacagagggc
      901 aaacccaagc tgatgtcagt cgtgtccctt ttcgatatgc cggaagagga cctgctgcga
      961 ttggtcgcta gcctggaacg caacagcgaa caccctctgg cctccgctat tgtgaacggg
     1021 gcgaaagaca aagggctgga gttagtgaag gctcaggact ttcagtcgaa aaccggcaaa
     1081 ggcgtgaccg gaaccgtcca gggacacagc atggcactcg gcacacggaa gtttttggag
     1141 gaagagtatc acgtgaggcc cgagaccttc gggcccttgt cggacaaggc cgaggacctg
     1201 cgccgtgacg gccaaaccgt catgtttgtg gccatcgcaa atcagattgc cggactcatc
     1261 ggagtcgccg acccgattaa accatcaacg tcggaagcca ttcgcctgtt gcatgaagaa
     1321 gggattcacg tggtcatggt atccggtgac catcagcaaa cagcacaggc ggttgcgaag
     1381 aagctcggca tcgatgaggt gaaagcggaa gtgttgcccg aggagaaaag ccggatcatt
     1441 caacaacttc aggcaaaagg aaaaatcgtg gccatggccg gagacggcat caatgacgca
     1501 cccgccctcg ctcaagccca ggtcggcatt gcgatgggga ccggcacgga tgtggccatg
     1561 gaaagtgcgg ggatcacgtt ggtcaaaggt gatcttcgag gcattgcccg tgcccgccgg
     1621 ctcagccagg cgaccatgcg caacattcgt caaaatctgt tctttgcttt tttctacaat
     1681 gccatcggag tgccgattgc cgccggcatt ctctatccgg cattcggcat acttttaagt
     1741 ccgatgattg cggcagccgc catgacgttt agctctgttt cggtcattac caatgccctc
     1801 cgattgcgaa ccacgacatt gtagttttta aatctgcaga attcatgaga ccactccagc
     1861 agcagagccg ggccttttac aatggtcaca atcgcattcc tcatccccat gaccacattt
     1921 taggtttttc aacacgacaa caatccaaaa tgaccgtaac ttaggatcat acggaaatta
     1981 tggctcgagc ccatcgacgg tactctcagg acaagctcta gaaggctccg tgcctctgct
     2041 tgtcatgctg agagaaacga ggatctgagc ccagtccaat caggccatgg ctcagccaga
     2101 tgcttcgcgc gcctgccctg agcggagtca aaggggctca gcataaccat tctgttgagg
     2161 gtgtctcgga tcatacagaa tagagggtac tatgctcttt tctgtgtgtc cctgaaaggt
     2221 tctctcccat ggtccgacgc ctgccgtccg gccctaagga gggacgctct ccttcgctgg
     2281 cgggccttgt ttgagcggag cgagttggtc cgccctccgc aggctgacgt ccctcccctc
     2341 tcatggggcc ggacggggcg tcaatggttt tggggccttt tgccgaaaca aaagggcctc
     2401 gcctgccggg gcgaaacccg gcaacacaga acattacgtt gacacgcgag ttgtggacgc
     2461 acgtgcgagg cgttcacctg ccagcgcttg ttaactggca attctcaaga tagatttccg
     2521 atacatcgtc aagctgtaag gcaagaacca taaattctga atgatccggt gtctcccacc
     2581 tcgttttctt ctcatcctca aacgtgtatg gatcccatca ggattcatct gttcagaatg
     2641 atcagctgta gggcgtagaa cgctatattt ttttagatcc gtagctgaca aacgtttatt
     2701 ccaaaagaaa atcaaaatct ttttgtgaga gagacgctcc gccgccatga ggggtggccc
     2761 cttccaggac cgcgtgatag agatccaatt tcttctgctt caattccatc attttctcct
     2821 cgatggtatg tcgcatgagt aaccgcatga tagacacgtt ctgcgtttgc ccgatgcgat
     2881 gcgcacggtc agacgcctga ttttccacgg ccggattcca ccacgggtcg aggtgaaaga
     2941 cataagatgc tttggtgaga ttcagccctt gcccgcccgc tttaagactc aataaaaaga
     3001 tagacgggtt ctctccctcc tgaaatttct gaaccagttt ttttcgggtg ggcgtcggtg
     3061 tggagccatc caatcggaca tgaggaatct catgcgcatc aaaggcctgt tcgacgatat
     3121 ccagaaacga cgtaaattga gagaagacca gcgcactatg cccttcatcc agaagctcct
     3181 tgaggcgatc aaggagacat tcaagcttcg gggaaagttc ggtatgcgcg ggactgagca
     3241 attgcggtga caagcagatc tgtcggagtt tgagaatcgc cgtcagcgca atgatccgcg
     3301 cctgcgcggg tgtttgactt cgataggcgg cagcaatggt cgaacgaatt ttggagaccg
     3361 tctgctgata taaactcttt tggcgatcag tcaattcaag atacagatcc gtctcaattt
     3421 tcggtggcag ttctttgagg atttggcttt tggtccggcg caacacaaac ggtctgctcc
     3481 gctgcatgat ggtctctaac aacgccggtg aaggcgcttt cattttcccc ctgatctctt
     3541 catagtctcc cagaagaccc ggcacgcaca aatccatcag ggcataatat tctcccacat
     3601 gattttccaa aggtgttccg gtcatcacgc atttaaaata tcccttgaga cgtcttgcgg
     3661 caccggtggt cctggctaca atgtttttga ccgcctgggc ttcatcgaac agaatgacat
     3721 tgaatgggat ctgttcgagg atcgcaatgt cccgtcggat caggccgtag gtggtgagga
     3781 ccacatcggc tccggcaaat gagggcgtgc gttccttccc ggtatacgac tgaacagtca
     3841 gtgagggata aaatctggaa atttcatgtt cccaattaaa taagaggctg gggggaagca
     3901 ccacgaggtg tggaccttga atcggttccg gtgcggtgat gaggccttct ttaatcccgg
     3961 cgaatagaca aatggcttgc agggtctttc ccaatcccat gtcatccgcc aggcacgcac
     4021 ccaggcgatg ttgatagaga aaccccagcc attgataccc ttcttgttga tacgggcgaa
     4081 ccgtcgcatc cacgacttga ggaaggggaa taggctcaat ctgctcaaaa tgcagcaacc
     4141 ggttgatcaa ggcttcatcc gccggaggga gaatgacggt caccccccgt tttctcaagg
     4201 ccacccaatc caggatttgc agtttcggga cacgaacgac agccttttgt tcccggtgtt
     4261 ccatgctggg cttattggag aggaaggcaa acgcgcgcaa aatggcctgg gtgatctgat
     4321 ccacaatccg gatgatgccg tccgcctcca tcattccacc ccgttcaaga attttttcga
     4381 tatccttggc ttcgatccgc accccgtcac acaacagttc cgggcgaagt tcaaaccaat
     4441 caatgcttga cgcccgtcgc gcatcgatcg agcaatccca ctgcgacgtg gtaatgggct
     4501 ggtcttgata gaatagcccg atgccgactt cctgcaattt tgcatgaagc tcgggaagca
     4561 ggggatagag gttggtgagc ggcagactca tttcatcatg ctccaccatt ccctcaaaaa
     4621 gcgaaggtcc ccacagcgta tatggaatgg catagagcaa ggcttctttc gcccaatcgt
     4681 tcatcaccac acaccagtga ccgtcgtgaa ccaccagccg cacactgggc tctagtgcgg
     4741 gcgaggaaaa tcgctgaagg atctgttcgg cctccgcctt cagtttgaat ggacggaatt
     4801 catcctgggc aatgcaggcc tttaccagtt tcctgacatc cgttggcttc tgatgaccga
     4861 tcaattggaa aaaggtgtca tagaggagtg cacggcgttt cttcgccttc aatccgctcg
     4921 agacggtttt ggctcgttca agaaatggaa atatccggaa aataggcaca ctggggcgcc
     4981 cctgactttc cccgagccaa cattccgcgc ggagaacccc tgtcctcaaa tccaacaccg
     5041 aggcatctaa cggaatggcc tccgggatga acgtgaggcg atactggtag gtcggtgcct
     5101 ttccggaatg gcggtgatca gtgagtgcaa cctcctgccc ggcacacttg agcaacaccg
     5161 accgcaggat tcggtcttgt tcatgaaggg gaatatccag ttgtaaggtg ataaattgtt
     5221 cgaccggaat ggaaaacgtg ggtctcgcca ttccagcagg gcgttccgct gaaagccagc
     5281 cttcgcgatg gtgggatgtc gccggcaggg ccaacggtcg gctaagcggg tccgcgggtt
     5341 caataacctg ttcccgctga ctgttctcat acagcagatc gtatacggcc catccacctt
     5401 catgttcgag tggcccaagc tttttggcat tcaaatcagc cgcaaatccc agacaggcgt
     5461 aggtgttcgg ttgcacctct ccgtagcgta aacaacaggc ggcaatcgaa acgtgtgacc
     5521 cgagtatgtc cagttctgtc ttggtggtat acagcgcatc cggtgcccat tccaaaggaa
     5581 atcgtccgtc cgcattctcg taaaacaagg catgatgctg gccgtgatgt ttcaggaaat
     5641 cccgtaaccc ttcctggacg gaccatgcgg gatcctgcgt ggcccgaagc agcacggcta
     5701 actcggtcgg catcccggca aaggattggc aaatgttccc atggttggta atgcgaatgg
     5761 atgacacgcc atgatggttg cagagagtaa tctcaaaccg tggaggagcg gtcggagtgg
     5821 atggttttga ggccaaaggg cttccggcgc gctccatgag ttgtcgcagg agtagggccc
     5881 gttgagtgga attggatgag ggcatacgga aagtatcggg aagcaggaga ttgactgtgg
     5941 tcaggagagc acaaatgaca tgcttacaat gcgactccgg cgtccacacc ggacaggtgc
     6001 aggaaaaggt cagctgcccg tgctggacac caaaccgaac cagatactgg gtggcccctc
     6061 gcaccgtcgc cgacaagata gactgcgtcc ggtcccagct ataagactcg agccttcctt
     6121 gggcataata ctcatatccg cgaaggacaa aggccttgga agccaggaag tagatatgat
     6181 tcggagagag gactttcagg tgttccagga caccaaaggg ttctacggac atcgttcccc
     6241 tatcatcgta taggaccctg agcaatcaac aaacaggcac gacctctggc accagggcat
     6301 tcccggtcgt gatggacaac gcatcactcg atgcacttgg taccgggtct gatgagacat
     6361 atgggtgctc tgcaaagcac gggtcccttt cgaccagaca tcccggcttg aacaacttta
     6421 cgggtgtttc cattccagat gcagatcggc aaagcttcaa ttatacacca ctcatcttct
     6481 gatgtatgcc gtttgaactg agctaatacc aaatatcgcc ataccggctc aaaacggttg
     6541 gtgagaaggc catgacgcgt tcattttgaa gattccacgc ctctctctgt tagcccggaa
     6601 tctcaatacg ttaaggaaaa gccaacgctc aggggaaaat gctggaaaat caacgctaac
     6661 cccaaaagca ttcctacgcg cccgcgccag gattcatctg taacatcatc agccccgtag
     6721 aaaaaaaatt ttccaccgaa ccggatcaag gtccggcttt cactcgtgca ggcagagagg
     6781 caagagggtt ggttaaggga tcggga
//
LOCUS       DATIAL010000384         5088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1576713, whole genome shotgun sequence.
ACCESSION   DATIAL010000384 DATIAL010000000
VERSION     DATIAL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576713"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..632)
                     /locus_tag="VLA60_11895"
     CDS             complement(198..632)
                     /locus_tag="VLA60_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HSF10110.1"
                     /translation="MDVLESSRGWEHFPHEADMGIRGFGITKPQAFEQAAIALTAVVT
                     DPLQVDSQLERHFTCDAPDDELLLVDWLNALIYEMACGHMVFGRFDVTITDHHLKAIA
                     HGEPVDRKKHEPAVEIKGATYTELSVRQIEGNGWVAQCVVDV"
     gene            complement(818..1021)
                     /locus_tag="VLA60_11900"
     CDS             complement(818..1021)
                     /locus_tag="VLA60_11900"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HSF10111.1"
                     /translation="MSDMLKVIEVLAESDKSWEDAASYAVTKAAKSVHGIKSIYIKDM
                     EAKVENNKITKYRINANISFLLD"
     gene            complement(1089..1490)
                     /locus_tag="VLA60_11905"
     CDS             complement(1089..1490)
                     /locus_tag="VLA60_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSF10112.1"
                     /translation="MHQPHNCLGFFIFILIMAWPILAFSQEVPGNPQNGKILFEKHCA
                     NCHGEKGAGDGPDTQFLLVQPANFHSLESRSKTDWELMNVITFGAVFSPMHGWSERLT
                     EDERWDVLRYIRFLAPFNPIAKLHPSAERHG"
     gene            complement(1564..2001)
                     /locus_tag="VLA60_11910"
     CDS             complement(1564..2001)
                     /locus_tag="VLA60_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10113.1"
                     /translation="MNHLLSLLLRSGSLALLILCMGMSPGWAQEQDVIAGGRQLFQKF
                     CVSCHGTQAKGDGPLSESLKPKPANLTRLSANHGGTFPFWHAYRTIDGRDHIPSHGTR
                     DMPVFGIWFRIPDDEVSIETEWADQVRGRIWQLLSYLESIQEP"
     gene            3028..>5088
                     /locus_tag="VLA60_11915"
     CDS             3028..>5088
                     /locus_tag="VLA60_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSF10114.1"
                     /translation="MAPKAKTPKPKGKPKKPAKKSSSAKKVAPPTSAKTPSKASRSSP
                     KLPSPVKNIPRSSQSREPRAGFPIVGIGASAGGLEAFEEFFTQMPPDSGLAFVVVPHQ
                     SPSHTSLLPGLLQKCTTMPVLEVADGLAVKPDTVYLALAGKNLALLNGRLQLMEPALQ
                     DRLPLPIDYFFRSLAEDQKHRAIGIILSGTGSDGTLGLKAIKGESGMTIAQEPQSAKY
                     TGMPQSAIATGIVDYVCPPTQMPEQILAYIRGPYLAPIPSPLDQESDAGRFLQKVFVL
                     LRDRTGNDFSLYKTNTIRRRIERRMNVHQIESPKQYLRYLVENPHELDALFQEILIGV
                     TSFFRDPAAFEVLAHQGLPSLLDDKPDGDLVRVWVPGCGTGEEAYSLAILLREYMIHR
                     KIRFQVQVFGTDLDAQAIEVARKAIYPGGIANDVTPERLERFFIKEESNYRVKKDIRD
                     LVIFATHNLLADPPFTKLDLLSCRNLLIYLEAQTQRRLFPLFHYALKGRGLLFLGSSE
                     TVGDFEEGHFESLERKWKLYRRKAGATDRTTLEGFPISRITPVRDLSFGEDCALPSPA
                     PSIPALIETMLLEQYAPSSVIVNERGEIVYIHGRTGAYLEPASGQPNLILVNMAREGL
                     RYPLAAALHQVSTQEREVVHQRIRVKTNGTFALVDLTVKPIQDPEALRGLYQVILESR
                     PAGAS"
BASE COUNT         1252 a         1316 c         1267 g         1253 t
ORIGIN      
        1 taactccaat cactcagccg ttccttcctt cactaggagc ctgtcggact taggaagaat
       61 cgactgcaaa agtgtctcag cggtccatat ttcaccgatt tcttggccca tagtcccact
      121 atgggcctgc gaaatcgtca aaatctggcc tcgctcagcc gctttttcgc tttcgatccc
      181 ctaagtccga cagactccta gacatccacg acacattggg caacccagcc gtttccctca
      241 atctgccgga cggataactc ggtataggtc gcccctttga tttctacggc tggttcatgt
      301 ttcttacggt cgactggttc tccgtgcgca atggctttga ggtgatgatc cgtgatcgtg
      361 acgtcaaacc ggccgaatac catgtgaccg catgccattt catagatgag ggcattcagc
      421 caatcgacca gtaatagctc gtcatccggc gcgtcgcagg tgaaatggcg ctccagctga
      481 gaatctacct gtaggggatc cgtcacgaca gccgtcagag ctatagctgc ctgttcaaag
      541 gcttgaggtt tggtgattcc aaaaccacga atccccatat ccgcctcatg gggaaaatgc
      601 tcccaccctc ttgaagactc aaggacgtcc atgtgtgtca ttagccggtt gtcagcctga
      661 gcttcagcaa aggaactgta cccagattta ccgtcccaaa gctcggccgg atccttccat
      721 gaaccacggc ctgagctccc tcgactgcgc ataggacagg attcccagat agtacaggat
      781 aactaagagc aggaatccat ctgtctttct tttctttcta atccagcaga aaagatatgt
      841 tggcgttaat tcgatatttg gtgatcttgt tattttccac tttggcttcc atgtccttaa
      901 tgtaaatcga tttaatgcca tgcacgcttt tggctgcttt ggtcacggcg tacgaagccg
      961 catcctccca actcttatct gattctgcta acacttcgat cacttttaac atgtctgaca
     1021 tggctgaacc tccttcttaa tgcgaaaatg aataataagc cactgacctg gagacagtgg
     1081 ttgagctgtt atccgtgcct ctctgctgaa gggtgaagtt tggcaatggg attaaaggga
     1141 gcaaggaatc tgatatagcg caagacatcc cagcgctcat cttccgtcag acgctctgac
     1201 catccatgca tgggagaaaa gacggctcca aaggtgatca cattcattaa ttcccaatcg
     1261 gtttttgatc gggattccaa cgagtgaaaa ttcgcgggct gtaccaacag gaattgggta
     1321 tccggcccat caccggcccc tttttctccg tgacaattcg cgcaatgctt ttcaaacagg
     1381 attttcccat tctgagggtt gcctggaact tcttgactga atgccagaat gggccaggcc
     1441 atgatgagga taaaaatgaa aaaaccaagg caattgtgtg gttgatgcat gatgtcctcc
     1501 tgctcgctta tctgtgaaga tgtggtttcg tgccatcgaa gtgtcatact cctcccctca
     1561 tatttatggc tcctgtatag attccaggta ggacagcagt tgccagattc gccctcgtac
     1621 ttggtcggcc cactctgtct caatcgagac ttcatcatcc ggtatccgga accatattcc
     1681 aaacaccggc atatcacggg tgccatgact tggaatgtga tcgcgcccat cgatcgtgcg
     1741 gtacgcgtgc caaaagggaa atgtcccgcc atggttggct gataatctgg tcagattcgc
     1801 cggcttgggc ttcaaagatt cagaaagagg gccgtccccc ttggcctggg ttccatgaca
     1861 ggaaacacag aatttttgaa agagctgtct ccctccggct ataacatcct gctcctgagc
     1921 ccatcccgga ctcattccca tacacaggat cagcaaggcg aggctcccgc tccgtaggag
     1981 aagactgagc aaatggttca tggggtatcc tttaagtgta aaaacattca tgcaacctct
     2041 tgagcatgtt tgggagagga tttgtattgg tattggcaaa ttttgcgatt ggatagggtt
     2101 tgcgaatcaa agatttcccc gcaattcaaa catcgccatt cataaatcca aacagtctta
     2161 tgaatatttt ctatattcag acaggtatcc atgaccatga acccttggca gcgagaacat
     2221 tccataatgt caccccccgg tataagtttt ttctcaaggt aggttcataa agcaatgaat
     2281 gtattaactc ctctctcgac ccatgtcgtc tcagcttgtg agcgagggag atcatagatt
     2341 cgataccaaa gaagatctat caaaccttat gccaaatcat gaatatggat tcaggacgga
     2401 aagaatgttg agagaaaaag gaagggaaag ggtgcctaat cccattgaga atagggcgaa
     2461 accctacata accagtgagg aaacattgcc ctggaaaaac agttaatctg gcatggcacg
     2521 gcggagaaaa gttcggggaa agatgcaaat gtcagtggaa ccgccaccgt actgtagcat
     2581 ctttccccag ccctttcgaa ccggtggtcc cagtcttcct cagtaagtgg tgcctgcctt
     2641 ttactgaaaa gaggtaggaa tcgccagccc tgccaagggg tctttgaatt ttttaagcgc
     2701 tggtgcctcc cgtggaggaa gatccccgat tttatcgcct gcgtccgtca tctccgccaa
     2761 tggtcagcgg ggattcattc ggaacgtagc caagatggac tcccgataac gaatgtcggg
     2821 agtgccagca gagtgaggct tgatgaacag tatcgggaat gccaacagaa aaattaggag
     2881 cggaaacctg gttgatcgat ataggttgag gctaactagg agggggaaat cctaaagagc
     2941 tgtacctttt ggccaaaaat gaatatgatt caacctccac attattacgg tgtcggtctc
     3001 acacttaccc ccaagggaat tgcttttatg gctcccaaag caaagacccc taaacccaag
     3061 ggaaaaccca aaaaaccggc aaagaaaagt tcttctgcaa agaaagtggc tcctccgact
     3121 tcggcaaaaa ccccatcgaa agcatcaagg tcaagtccta agcttccctc cccagttaag
     3181 aatatccccc gttcctctca atcccgggaa cctcgcgcag ggtttccgat tgtggggatt
     3241 ggcgcctcgg cgggcgggct cgaagcgttt gaggaattct ttacccagat gccaccggat
     3301 agcgggttgg ccttcgtggt ggttccccac caatccccca gtcataccag tctacttcca
     3361 ggcctcctgc aaaagtgtac gaccatgccg gtcctggagg tcgcagatgg tttggcggtg
     3421 aagcccgata cggtctatct ggctttggcc gggaaaaatc tggcattgct gaacggtcgg
     3481 ttgcaattaa tggaaccggc cttacaagac cgactgccct tacctatcga ttactttttc
     3541 cgttccctgg ctgaagacca aaaacatcgg gcgattggca tcattctgtc agggacgggg
     3601 tccgatggaa cattgggttt gaaagcgatc aagggggaat cgggcatgac gatcgcccag
     3661 gagccgcaat ccgccaagta cacggggatg ccacaaagtg ccattgcgac cgggattgtc
     3721 gattatgtct gtcctccgac acagatgccc gagcagattc tggcttatat ccgaggtccc
     3781 tacctggcgc ccataccctc gcctctggac caggaaagcg acgcgggacg gtttttgcag
     3841 aaagtgtttg tgctcctgcg tgatcgaacc ggaaacgact tttccctgta taaaacgaat
     3901 acgatccgcc gccgtatcga gcgacggatg aatgttcacc agattgagag tcccaaacag
     3961 tacctccgct atttggtaga aaatccgcac gagttggatg cgctgtttca agaaattctg
     4021 atcggcgtca caagcttttt ccgggatcct gcagcattcg aggttctcgc ccatcagggc
     4081 ctcccttccc tcctggatga caaaccggat ggggatctcg tgcgggtgtg ggtccccggc
     4141 tgtgggaccg gcgaagaagc ctattcgctg gctatcctcc ttcgcgaata tatgatccac
     4201 cggaagatcc ggtttcaggt ccaggtcttt ggaactgatt tggacgccca ggccatagag
     4261 gtggcgcgga aagcaattta tccggggggg attgccaatg atgtcacccc ggaacgattg
     4321 gagcggttct ttataaaaga agaaagcaat taccgtgtca aaaaagacat tcgggatctc
     4381 gtgatttttg ccacgcacaa cctgcttgcc gatcctccct tcacgaaatt ggatctgttg
     4441 tcatgtcgga atctgcttat ttatttagag gcccaaaccc aacgtcgcct gtttcctctt
     4501 tttcattatg cattgaaagg gagggggtta ttgtttctgg gatcttcaga aacggtcggg
     4561 gattttgaag aagggcattt tgagagcctc gaacgaaaat ggaaactgta tcgacggaaa
     4621 gccggagcca ccgatcgcac cacgcttgag ggatttccca ttagtagaat caccccggtg
     4681 cgtgaccttt cattcggaga ggattgtgct cttccgagtc ccgccccctc cattcccgcc
     4741 ttgattgaaa cgatgttatt ggagcagtac gctccttcca gcgtaatcgt gaatgaacgg
     4801 ggggaaatcg tctacatcca tggacggacc ggcgcctatt tggaaccggc gtccggccaa
     4861 ccgaacctca ttctcgtgaa tatggctcga gaggggttac ggtatcccct ggcggcggct
     4921 ttgcatcaag tctccactca ggaaagggaa gtggtgcacc aacgcattcg agtcaaaacc
     4981 aatgggacgt ttgccttagt ggatctgacg gtcaagccga ttcaagaccc tgaagcactt
     5041 cgagggctct atcaggtgat attggaatcc aggccggccg gggcctcc
//
LOCUS       DATIAL010000385         3764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1145588, whole genome shotgun sequence.
ACCESSION   DATIAL010000385 DATIAL010000000
VERSION     DATIAL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145588"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..659)
                     /locus_tag="VLA60_11920"
     CDS             complement(204..659)
                     /locus_tag="VLA60_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10115.1"
                     /translation="MNACSAEGLFQVDGEKGVVEWGEEGVKAMATILVVDDDQQVCAL
                     LKQVLEEEGYTVESALNGMEGISRYRSVPTDLIILDILMPEKEGLETILDLRREFPKV
                     KIIAMSGGSERAKLNLLDLARRLGAQYTVNKPFQLQTLTDLVKKALQED"
     gene            complement(824..2812)
                     /locus_tag="VLA60_11925"
     CDS             complement(824..2812)
                     /locus_tag="VLA60_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10116.1"
                     /translation="MTAHSEWSRILIVEDHPGQLEILAELLGKEGYHVLGVSTVAEAV
                     RYAKNEKFLVAVVNLQVPDASGIQLFHLLKRMNGRIRVMMNSAFANLNLVEEGVNGDT
                     FTYLEQALSPSELLGHIRHVFREQIFEYIQDLETALAERTAELAERDERLGKLVEHIN
                     EVFWISTSDQSEILYVSPRYETMWGRSCASLFQDPRSLWKAVHPQEQERLLTHQSEAN
                     VAGFQGECRIVKPDNSIRWVRIRTFPLKNPDGDILYLAGVAEDITEQKSLEEALTKTE
                     RQFRQSSRMEAIGTLAGGIAHDFNNILTAILGYTELALATVAKGSRTQRNLQEVLTAG
                     HRAKHLVLQILAFSRQTGQGKKPTPIHMIIREALKLLRATIPSTIEIRQSLKTEATIL
                     ADPTQMHQVIINLCTNAEYAMRETGGILKISLKDVEVTEDLARMISGLQVGPHVQLTV
                     NDSGTGMTPEILERMFDPFFTTKPIGEGSGMGMAVAHGIITGHGGAIVVDSVVNKGTK
                     IEVYLPTIPTPVWERVMDHGPIPIGKETILFVDDEETIVHLGKELLTQLGYTVEVYTN
                     SVEALGAFRHDPHRFDLVITDQTMPVLTGEALSRELLQIRPDLPIILCTGFSHIMSAE
                     KAKALGIQAYLMKPLAIRDLVPIIRHVLDKTPSSLAGP"
BASE COUNT          992 a          991 c          756 g         1025 t
ORIGIN      
        1 cgtaatcagt gcgctgtagg gtgtaaacat catcccgagc actgggtatc ccaccgtcca
       61 ggcatcaccg tcattcgccg aagaaatacg acggattcct tgaagctccc aagagagagc
      121 atgcttcaca aactcgataa acaccgaatc gggttgtgtc cgcaaataca gcatggctcg
      181 gtactccaac gggtgaaccg gtactaatct tcttgaagag cttttttaac cagatccgtg
      241 agggtttgca attggaaagg cttattgacc gtatattggg cccctaaacg gcgagcgaga
      301 tccaacaaat tcagtttagc ccgctcactt ccccctgaca tcgcgataat tttgacttta
      361 gggaattcac gtcgcaaatc caggattgtc tcaagaccct ctttttcggg cataaggata
      421 tccaagataa tcaggtcggt cggaacactg cgataccgac tgattccttc cattccattt
      481 aaggcactct caaccgtata cccttcttct tccaaaactt gtttcaaaag ggcacaaacc
      541 tgctgatcat catcaacaac taagatggtt gccatcgctt tgactccttc ttcgccccac
      601 tccacaacgc ctttttcccc atcaacctga aaaagaccct ctgcggaaca tgcgttcaac
      661 tttggcatct ccatgaaaaa taattggttg aagtttagaa aatttctgac ttcatctcga
      721 acctcatctt ccgcaataat aattcttttt tacagtatga ttgaatgttc atcgatcgct
      781 cggaataact gattttttct tttttccatg acctcctatc ggtttacggt ccggccaacg
      841 aagagggggt tttgtcgagt acatggcgaa taataggaac caggtcccta atggccaagg
      901 gtttcatcaa ataagcctga atacccaatg ccttagcttt ttctgcagac atgatatgac
      961 tgaatcctgt acacagaatg atgggcagat ccgggcgaat ttgtaaaagc tctcgggata
     1021 aagcctctcc agtgagaacg ggcatggttt gatcggtaat caccagatca aaccggtgtg
     1081 gatcatgacg aaatgccccc aaggcctcta cggaattcgt gtagacttcc actgtataac
     1141 ccagttgggt gagaagttcc ttcccgagat gcacaatcgt ctcttcatca tccacgaata
     1201 aaatggtttc ttttccaatc ggaatgggac cgtgatccat cacccgttcc cataccggtg
     1261 tgggaatcgt ggggagatac acctcgattt ttgtcccttt gttcacgaca ctatcaacca
     1321 caatggcccc tccgtgccct gtgatgattc catgagctac cgccattccc attcccgacc
     1381 cttccccaat aggcttggtg gtgaaaaatg gatcaaacat tcgttcaaga atctcaggcg
     1441 tcatgcctgt gccgctatcg ttcactgtca actgaacatg cggaccaacc tgtaagcctg
     1501 agatcattct tgccagatcc tccgtgactt ccacatcttt caaggagatt tttaaaatcc
     1561 cgccagtttc ccgcatggca tattcggcat tggtgcataa attaatgatg acctgatgca
     1621 tctgggtcgg atcagctaga atagtggctt ctgtttttaa agattggcgt atttcgattg
     1681 tagagggaat ggtcgctcgt aagagtttaa gagcttcccg gataatcata tgaatgggtg
     1741 tgggcttttt cccctgaccg gtttggcgac taaatgccaa gatctgaagg accaagtgct
     1801 tggctcgatg tccagccgtc agcacctcct gaaggttacg ttgagtgcga cttccctttg
     1861 ccacagtggc caacgccagt tcggtatacc ccagtatggc cgttaaaata ttattaaaat
     1921 catgggcaat acctcccgcc aacgtgccga tcgcttccat gcggcttgat tggcgaaatt
     1981 gacgctcggt tttcgtgaga gcttcttcaa ggcttttctg ctctgtaata tcctctgcca
     2041 ctcccgcaag ataaagaata tctccatctg ggtttttcaa aggaaacgtg cggatccgca
     2101 cccaccggat tgaattatcc ggcttcacaa tccggcattc tccctgaaat ccagccacat
     2161 tcgcctccga ttggtgcgtc aggaggcgtt cttgttcttg gggatgaaca gctttccaca
     2221 gacttcgagg atcctgaaaa agactggcac aacttcgtcc ccacatggtt tcataccgcg
     2281 gactgacata caggatttct gactggtcag aggtagagat ccaaaaaacc tcattaatgt
     2341 gttccacaag ttttccaagc cgttcatccc gctccgccaa ttcagcagtc cgctctgcca
     2401 gggctgtctc caagtcttgt atatattcga aaatctgctc acgaaagacg tgccggatat
     2461 gcccaagcaa ttcggatggc gagagcgcct gttcaagata ggtaaaggta tccccattca
     2521 caccttcttc gactaaattc aaattggcaa aagcactatt catcatcacc cgaatccgtc
     2581 cattcatcct ctttaggaga tggaacaatt gaataccaga ggcatccggc acctgcaaat
     2641 tgacgactgc tacaagaaat ttctcatttt ttgcatatct cacagcctcc gctaccgtag
     2701 agacgcccaa cacatggtag ccttcctttc ctaacaattc agcaaggatt tctaattgcc
     2761 ctggatgatc ttccactatg aggatacgtg accattcact gtgagctgtc atacgttcct
     2821 ccataaagca tactgttaaa aactattccg gtaaggctca gttttggcta gtagatttga
     2881 caatcatttt accaattctc tttcatccac cccgggatgg cgaacagtct ttcttcagcg
     2941 gaattaagac ggaattcttc cccaatctcc aacgcatcat gcagataccc ttaccgcacc
     3001 tcttactgta tccgttcaca tcaaaccagc ctcaatgaag atttgcgcac ctcaatagag
     3061 tttttaagtc tccgtaacag tgatctaatc ccttcttgtt cagatctcgg taagacttac
     3121 tcgcatagtt ttatgtcccc aaaaactttg tttcgtcgga tattattggt gactttcata
     3181 tattccggaa agcagcttct cattctttct caaatccagg aatcatcata ccaaccgggc
     3241 gcatgaatgg tcacgctgtt gcccctaagg ctatcagcgt aaatttatat gaacgccgta
     3301 tcctgaagaa tcacatcaat tagatcatgc ttaatgtcca actgctaaaa atttcaccat
     3361 aaggctaata taggcatgag atagagtggg tattctaaca cggacaaagc ctagccctga
     3421 gtatttacat caaccatatc taactcagga caactcatga tagtaaaacg actggcgcac
     3481 aggagaatgg cctttccatg ctaccgcgcc atatacagag gaaaactaag cttcgaactt
     3541 tgctaaaaat cagtgtcctg attcctcaag cctcgtgtca tagaacttct aactctccac
     3601 accaattctc atctatttcc ggatgaactg gcccttaccg ctactccaat ctcctcttga
     3661 atcttttgag ccatatcccg ccgattggca gcatcccgaa taggtcgacc caccactaaa
     3721 taatccgcac ctgcttgaat ggcgtcaaaa ggagtcaagg aacg
//
LOCUS       DATIAL010000386         5146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_178694, whole genome shotgun sequence.
ACCESSION   DATIAL010000386 DATIAL010000000
VERSION     DATIAL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178694"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(700..1449)
                     /locus_tag="VLA60_11930"
     CDS             complement(700..1449)
                     /locus_tag="VLA60_11930"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HSF10117.1"
                     /translation="MTNIFFDFTITQLLLVGVLFIWTGFVRSGLGFGGAALGLPLMLF
                     LYDQPLFWLPVIGSHLLFFSALTLRTGIHDVDWPYLRHATLFVIPTTLIGVFGLITLP
                     TEWLLIFIYSISLAYGLMWLLNLSINSKKSWSDRLLLALGGYVAGTSLTGGPLMVAVT
                     MKNVATNQLRNTLYVLWFTIVMIKMSTFVILGVELHLLTALTLLPIAAIGHVIGLKFH
                     DAILKNDQWFKQVIGGMLVLISGLGLMNNLA"
     gene            complement(2592..3224)
                     /locus_tag="VLA60_11935"
     CDS             complement(2592..3224)
                     /locus_tag="VLA60_11935"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HSF10118.1"
                     /translation="MSTYFRHVYRFLIRPWIAPTVRLYLLRTSVAEKGLRESPSQPHP
                     WQTIWGLCVVMLMGGLFTGCVTDATTDLTKAPFDASTELTDASTDSITRLTDGTSQAT
                     TDLTEPTREFLSSTTPGAWFNADGMLNPDHKKIAFVVVNFDNLQEDIARGSGEYLFSL
                     SRLIGVPPDSRDSFMQSAQRQHAYIFDESQSRPESFRRLIRTLHVTSPYS"
     gene            complement(3601..3813)
                     /locus_tag="VLA60_11940"
     CDS             complement(3601..3813)
                     /locus_tag="VLA60_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10119.1"
                     /translation="MNCPRCGGFQMLDQFYGLENNGSVWMYDAVRCLNCGSIEEPMRE
                     KGETISPPRAERINQYALTVRTVAAR"
     gene            complement(4234..4899)
                     /locus_tag="VLA60_11945"
     CDS             complement(4234..4899)
                     /locus_tag="VLA60_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HSF10120.1"
                     /translation="MEPKRRPSFNPKTFLRQVGEGKTILSCKKNQVLFSQGDTAEAVF
                     YILEGKVTLTVVSKRGKEAIVAILEPATFFGEACLTGQLLRLEAATTVGVAKIVRIGK
                     TAMIKILHDQPTFSELFLTHLLARNIRIQEDLVDQLFNSSEKRLARVLLLMAHFGKES
                     KPEAVIAKLSQETLAEMIGTTRSRVSFFMNKFRKLGFIEYNSQSDKELHVHSSLLNVV
                     LHD"
     gene            complement(4966..>5146)
                     /locus_tag="VLA60_11950"
     CDS             complement(4966..>5146)
                     /locus_tag="VLA60_11950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10121.1"
                     /translation="VLQHIQERYPSIPVVMLTGHTECHLEAQAIAAGARACLYKPFNW
                     VELEGILRCSVGAAS"
BASE COUNT         1264 a         1353 c         1328 g         1201 t
ORIGIN      
        1 tctgattata tgcgcctggc cattgtcatg gcgcaaaagg tcccccaata tcccttcgga
       61 gccgtgaccg acctgggcga gccggaagat atcggcaaat tcttttccgg ctttcaaaaa
      121 tacctctacc agccacaagc cgtcgcatag gaacagggcc gggccattga ccggggttgg
      181 cattgcctcc tcacttactt ccaaccacct tcccacattc ctcattcatc agaatggttc
      241 actgttctct gcgggatctt gttgcacttg tcggttagat gccgaggttc ggcccggcag
      301 ccgagccact tttgtttcgg caaaagtggc caaaaccatt gacgccccgt ctggactcat
      361 tgaagaggag ggacgccaac ttagaagaag gcggaccaac tcgcaaggct caaacagggt
      421 ccgctagcgg agaagagcgt ccctccttgg ggccagccgg caggcgtcgg aatacagacg
      481 aaaggttcat gcttgaggtc cggccttgca ttcacacgta cttccaaccc ccttccaggc
      541 ttccgcaaaa tatagaatag ttagcttttc tctgcgggtt cttctagggg aaaagtttgc
      601 gcccttcggc atgcctcagg ccaggcatcc cgagtttgat cgcccccctg tattgaaccc
      661 ctagataatg gtcatggggt cacacatgag caggctgtct taggccagat tgttcattaa
      721 tcccaatccg ctgatgagga cgagcattcc tcctatgacc tgtttgaacc actgatcgtt
      781 tttcaatatc gcatcatgaa atttgagtcc gatcacatgg ccgatggctg cgatgggaag
      841 gagcgtcagg gcggtgagca gatgcagctc gactccgagt atcacaaagg tactcatttt
      901 aatcatgacg atggtaaacc agagaacgta caacgtgtta cgtagttgat tggtcgcgac
      961 atttttcatg gtgaccgcaa ccatcagtgg tccaccggtc agtgacgttc ccgccacata
     1021 cccaccaagc gcaagcaaca gcctgtcgct ccaactcttc ttgctattga tggaaagatt
     1081 caagagccac atcaatccat acgcgagtga gatggaataa ataaagatga gcagccactc
     1141 agtcggcaac gtaatcaggc cgaacacacc aatcaaggtc gtggggatga caaaaagcgt
     1201 ggcgtgccgc aaatagggcc aatcgacatc gtgaataccg gtacgtaagg tcagagccga
     1261 aaaaaacagg agatgcgatc cgatgaccgg caaccaaaat agtggttggt catagagaaa
     1321 cagcatgagt ggtagtccaa gcgcagcacc accgaaccca agcccgctgc gtacaaaccc
     1381 tgtccagata aacaggactc ccacgaggag tagctgagtg atcgtgaagt caaagaagat
     1441 gttcgtcatg gatcgtgcgt ttgctgaaaa aagccgccaa cggcgttccc tgccttcccg
     1501 ctcgccgcta aagccagctt tggcgcgcaa gcggccgatg cggccacgcg tttacgcgct
     1561 gaagcgcttc aacgcgcaag cgggcaggtc ggtttccaat tttaagttcc cacgtactgt
     1621 ccgaaatacg ggtccagtca gtttataccg gttatatcat gcaacacatc acgaattttc
     1681 gctgcaaggc ttctcaacgt accatttccc tcacagtcag gtctgtttta cagaatgatc
     1741 tatcccctac aggcaaagat ggccacatac atttgaagtg tgacaggttt gggggtccgg
     1801 ccttttattc acacagactc ccagcccatt tcccacattc ctacttgttc agaatgattc
     1861 actgctctct gcgggttctt gtgggacttg tcttttagag gccggggatt cgcccccgaa
     1921 cgacgaggtc cttttgtttc ggcaaaagga cccaaaacca ttgacgcccc gtctggcctt
     1981 attgaagagg agggacgcga acattttgaa gagcggacca acttgcaggg ctcacacaag
     2041 gcccgctaga tgcgaagagg gtccctcctt ggggccagcc ggcaggcgtc ggatccttag
     2101 gagacgaaca gtttagggac ttccaggaaa aagcttggac tgtaggaatg tggagtgaag
     2161 aagatcgatt cccgggtttt ggctcggcag ccgagccact tttgtttcgg caacagtggc
     2221 caaaaccatt gacgccccgt ctggcctcat tgaagaggag ggacgctaac tctgcagagg
     2281 ggggaccaac tcgccaggct tgtcctgagt tttcgaaggg ctcaaacaag gcccgctggc
     2341 tgagaagaac ctccctcctt ggagccagcc ggcaggcgtc gaaccaaagc ggatgtacca
     2401 ttaggaattt ttataaaaga gagctgactt cagaagtgac ggagtggtcc ggataatgaa
     2461 cgtcgaatgg tatgtcctac ccctttgtgc gaaatgaaaa cgagaatcag acgaaaacga
     2521 taccgccttg ttcaagacca tgcggagaca cgctcagcct ttctaggtcc tctagataag
     2581 atcttcctcc cctacgagta gggtgacgtc acatgtaatg tcctgatcag acgtctaaac
     2641 gattccggcc gggattggct ctcatcaaaa atataggcat gctgacgttg tgcggattgc
     2701 atgaaactgt cgcgcgaatc agggggaaca cctattaacc gagaaagaga aaacagatat
     2761 tctccggatc ctctggcaat gtcctcttgc aaattgtcaa agttgacgac cacaaacgcg
     2821 atctttttat ggtcaggatt gagcatgcca tccgcattga accacgctcc gggtgtcgtg
     2881 ctggaaagaa attctctagt gggctcggtg agatcggttg tcgcttgact ggtcccatcg
     2941 gtcagtctgg taatcgaatc cgtcgaagcg tcagtcagct cggtggacgc atcaaacggc
     3001 gctttggtta aatcagttgt ggcatcggta acacatcccg tgaataaccc gcccatgagc
     3061 atgacgacac acaggcccca tatggtctgc catggatgag gctgactggg gctttcacgt
     3121 aaaccttttt ctgcaacaga agtgcgcaga agatagaggc ggactgtcgg tgcgatccac
     3181 ggccttataa gaaaacgata tacatgacga aaataggtac tcatgcgcga ttcccccctt
     3241 gattagaaaa atgaatgatg gatatatgaa ttatttaaca ttatataaaa tcacctgctt
     3301 ccattctttc cccaaattca tactggaagc atggtatttt gtattcttgc cccgctccgt
     3361 gggtttttat tttttcgcac gtctttgcgc ggggtggacc gagagggctt caccacatcc
     3421 acccggggcc atgtggccgg tgggggcctt cacgcctcct gacggacatg ctcccgaagt
     3481 tccaatattt cgagatgttt ggaatcttga acaaaccgag tggacggaat ccatgctgca
     3541 ctgtcttcat ggtcaaggca gatgacaagg agaggccatg gaaagcggtc ctccttttac
     3601 ctatcgggcc gccactgtcc gcacggtcaa ggcatattga ttgatgcgtt ctgccctggg
     3661 cggtgagatc gtctcccctt tttctctcat gggctcttct atcgatccac aattgaggca
     3721 ccgcacggca tcatacatcc agacagatcc attgttttct agaccgtaaa actgatccaa
     3781 catctgaaac cctccgcaac gtggacaatt catacatgcc tccttgttgg gtgctacttt
     3841 ccatttgaat tagttgttcc tccccaggcc ttttccctat aaggtcgaca gttgttcggc
     3901 ctacgtcaca ggcctcgcct ccctccgccg gcatggttca gcgaaagatg caacccacag
     3961 cacactcatc atcggcggta cgcccgaagg ggtatccatg gcgtgcgcgc tttaacattc
     4021 aatctatttt tgcggagcgc gagagcatac cctgtgaccg gtgtgaattg ctctaactca
     4081 taatcatagg cctccgtgca aggcttgtct ggtcaatatt gctcaggggt gaggacggaa
     4141 acggagggtt ccttgaggtg cccccggctc aacttcattg aaccgactga acggcaaggg
     4201 aagcggaagg gagaccaaac cggaggagga attctagtcg tgaaggacga cattcaggag
     4261 ggaactgtgc acgtgcaact ccttgtccga ttggctgttg tattcaataa agccaagctt
     4321 tcgaaactta ttcatgaaga aactgacgcg ggagcgggtg gtgccgatca tctccgcaag
     4381 cgtttcctga ctcaatttcg caatgacggc ctccggcttg ctttctttcc caaaatgcgc
     4441 catgaggagc agcacacgcg ccagccgttt ctcgctggaa ttgaaaagtt gatcgactaa
     4501 atcctcttgg atgcgaatat tgcgagccag gagatgcgtg aggaagagtt cagagaaagt
     4561 gggttggtca tgaagtatct tgatcatggc ggttttgccg atgcgaacga ttttggcgac
     4621 accaaccgtg gtcgcggcct ccagacgcaa tagctgcccg gtgaggcagg cttcaccaaa
     4681 gaaggtggca ggctccagaa tcgccacgat ggcctctttt ccgcgtttgg agaccacggt
     4741 gagtgtgacc ttgccctcca aaatatagaa gacggcctca gccgtatcac cttgtgaaaa
     4801 gagaacctgg ttttttttgc acgacaagat ggttttgccc tctccgactt gcctcaaaaa
     4861 ggttttcgga ttgaacgagg gtcttcgttt aggttccata catccttttg aaataagatg
     4921 ggtaatgaat tctcgataac gtcaggcact gggagaccat tttccttatg aagccgcgcc
     4981 gaccgagcaa cgaaggatgc cttccaactc cacccaatta aaaggtttat acagacaggc
     5041 ccgcgcccct gccgcaatcg cctgggcctc caagtggcat tcagtatggc cggtgagcat
     5101 cacgaccggt atggacggat accgctcctg gatgtgctgt aagacc
//
LOCUS       DATIAL010000387         7174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_931349, whole genome shotgun sequence.
ACCESSION   DATIAL010000387 DATIAL010000000
VERSION     DATIAL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7174
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931349"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VLA60_11955"
     CDS             <1..317
                     /locus_tag="VLA60_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HSF10122.1"
                     /translation="QRLPTELYQSLTWDRGTEMANHRQFTLATDIAVYFSDPKSPWQR
                     GSNENTNRLLRQYFPKGTDLSLYTQAQLNKVARQLNERPRKTLEYETPAEKFQACVAA
                     TS"
     gene            complement(367..1248)
                     /locus_tag="VLA60_11960"
     CDS             complement(367..1248)
                     /locus_tag="VLA60_11960"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF10123.1"
                     /translation="MNATDALDKMEAKVDELPLLPMVLVKIMQLDPNSEDYYDEFVRL
                     LEEDPPFAVRVVALANSAAYHPVSPIVRIKDAVTRLGSDTVRSLVASLAVQRVFVPTS
                     QNEIKLWTHSISTAVTSKHLAKMIPKLEVNPEKAYLCGLLHDIGRFVMFEHAPQHLKD
                     VDETHWQTPEELIEADVEVFKYAHTDLGYRACKNWGLPEEVAVVVRDHHNHTLDKPTK
                     PATYEATVACVRLADHLTVAVLENRGTDSDFQRVIEEHVLVSSEEKNWLDAASLVSRL
                     DAIRSEIDGLQKGMGFN"
     gene            1643..2011
                     /locus_tag="VLA60_11965"
     CDS             1643..2011
                     /locus_tag="VLA60_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10124.1"
                     /translation="MRSFPKLLALFLIGLFLAFSFNAYACVVPVFAKMPVAQTQGSDC
                     TMPGEEPVTQFCDGFKTLAVQWSLDTPSNSQTHAPFIGEMALLLPDLVPSSRKIIFPP
                     GTGHVAVPKDLLLLISVFRI"
     gene            2194..3459
                     /locus_tag="VLA60_11970"
     CDS             2194..3459
                     /locus_tag="VLA60_11970"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF10125.1"
                     /translation="MISRYVVIMAFGMVWLGLPGPLSAQPTGTTEPVLELLPLIQEAI
                     QQNPEIAATQEQVLAMKERAPQVGALADPELKVQLWNTPDSLNVTKTQTVIYGVAQQF
                     PFPGLLSKKAEVATREADQAEQRMAAKIREITAAVKMAFFELLFAHKAIDVHHEQQKL
                     LRQFFDIANAKYGVGTRTQVDVLRAQVELSKISQQFPVLNQRRETAQAHLNTILNRDP
                     HAPLGIPRAPHPQPLTRSLEELQEHALRLRPELQEADLAVTRFQSATQLAKLQYYPHL
                     RVELQRWQNYHTDDGFGGNVMVNIPFAFWTKPKYDAGVREAAAQVRSAQAKQQTLVTL
                     TRFQVKDLVVQIQSTQEVLELYRTTVLPQAQQVLQAAHAGYRTDRTDFLDLIDAERAL
                     ITFRLEYIRALVDREQQVAQLERVLGADL"
     gene            3474..5156
                     /locus_tag="VLA60_11975"
     CDS             3474..5156
                     /locus_tag="VLA60_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HSF10126.1"
                     /translation="MLTHISRPRWIILGILLCILVASLFFFKESWWPLFHSTGTTTKE
                     AKHIEQPMQDMEAMSSHSSMERESSQAYAMVTPARQQLIGVTTAPVSMRPLRTVIRAV
                     GKVDYDEQRLTHINLRISGWVEELFVDTTGQLVRKGQPLFTLYSQELVSAQEEYLLAM
                     KANKQIQNSPLLETRHQTAQMVQSARDRLRHWTLTDAQIDDLARRGKPQTYVTIYSPA
                     AGYVIDKKVFKGTLVKPEMTLYAIADLSTVWVEAEVFEYEMPFVQVGQEGILTLAAYP
                     GETFRGEITFIYPYLNREARTNTVRLVFKNPKLRLKPDMYGTVQIQVNRGSKLAVPEE
                     AVLDSGIRQIVYVVRGEGMFEPRQVTLGPKVGGYYEVVEGLALDERVATSGTFLLDSE
                     SKLMASSNMMGALGMGGIKMEQAHMGKMDMGMDKDKNMPQTKTATAKNNVSSGKMERK
                     AEGLTLILSTDLTPVRMGENRIQMTLTDAAGKPASHAKVRLTFTMPMPGMIPATVPMT
                     EEKPGIYVAKVNLGMAGQWDLTIKIQRSDQPDVKERFSVIAVGSGMPGMPGM"
     gene            5340..6008
                     /locus_tag="VLA60_11980"
     CDS             5340..6008
                     /locus_tag="VLA60_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HSF10127.1"
                     /translation="MAADGIPHDHKAIVDIFRKDIQRLEAGTTLSAERATKLKRMAKD
                     PKGVQRPLLLNQANKNSQQGGINMEQAQLWQMNIGGKDTQSTDMRVKVTDNRRLIPGT
                     SNDTYASPGKMETLADGLAMIFTTEPSPPRVGENRMVATVTDATGQPVSRARVRLTYT
                     MPFQNMTPTTVRLREGTKGVYEAIVNLGLGGLWDLQVGIIRPGQPEIQETYSVTAGGG
                     DLLE"
     gene            6030..>7174
                     /locus_tag="VLA60_11985"
     CDS             6030..>7174
                     /locus_tag="VLA60_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF10128.1"
                     /translation="MIERIIGWSARHGFLVGILTLFLMGWGIWAVYHTPLDALPDLSD
                     VQVIVLTEWPGRSPDLIEDQITYPIVTSMLGAPRIKYVRGQSFLGLSFVYIVFQDGTD
                     MYWARSRVVEYMQGLVGKLPEGISPTLGPDATGVGWVFQYALVDESGNHSLADLRSFQ
                     DWYLRYWLQSVPGVAEVATIGGFVKQYQVQVDPVKLQGYGIGLPQVIQAVRRSNNEVG
                     GRVIEASEREYMVRGRGYIQSLDDLRTIPIGMDGKGTPITVHDVATVTIGPDLRRGIA
                     ELNGQGETVGGIVVMRYGENALEVIQRVKEKITTITPSIPEGIRIVPVYDRSELILRA
                     IATLKEKVLEITIVVSVVSLLFLFHVRSALVPVLLLPVAILLSFIAM"
BASE COUNT         1806 a         1876 c         1834 g         1658 t
ORIGIN      
        1 cccagcgcct ccccaccgag ctgtatcaat ccttgacgtg ggaccgaggc accgagatgg
       61 cgaatcatcg ccaattcact ctcgcgaccg atattgcggt gtacttttct gacccaaaga
      121 gcccatggca gcgtggatcc aatgagaata cgaaccgcct gctcaggcag tactttccca
      181 aaggaactga tttatccctg tatacgcaag ctcagctgaa taaagtggct cggcagttga
      241 acgaacgccc gagaaaaaca ttagaatacg aaaccccagc ggagaaattt caggcctgtg
      301 ttgcagcgac cagttgaact caccacccaa agcgggcatt cactaaacgt agaaacaaag
      361 acaaagttaa ttaaatccca tccccttttg caggccgtca atttcagagc gaatggcatc
      421 taatctggat accaatgagg cagcatcaag ccaattcttt tcctcactgg aaactaacac
      481 gtgctcctca atgacccgtt gaaagtcgct atcagtgcca cggttctcca ggacggccac
      541 agtcaaatgg tctgctaatc tgacgcaagc cacagtggct tcataggtag ctggttttgt
      601 gggtttatcg agtgtgtggt tgtgatgatc acgaacaacg acagcaactt cttctggcag
      661 accccagttc ttgcaggcgc ggtagcctaa atccgtgtgg gcgtacttga atacttcaac
      721 atcagcctct attaactcct ctggcgtttg ccagtgtgtt tcgtcaacat ccttgagatg
      781 ttgcggcgcg tgctcgaaca tcacgaatcg accaatatca tgcagaagcc cacataaata
      841 ggctttctct ggattaacct ccagcttagg aatcattttt gccagatgtt ttgacgttac
      901 cgctgtggat attgagtgtg tccacaattt gatttcgttt tgacttgttg ggacaaaaac
      961 cctttgcact gcaagcgatg cgaccaggct tcggacggtg tcgctcccta atcgggttac
     1021 cgcgtccttg attcttacta tcgggctaac agggtgatat gcagcggaat tggctaatgc
     1081 cacaactcga acggcaaaag gtgggtcttc ttctaaaagt cgcacaaatt catcgtagta
     1141 gtcttctgag ttgggatcga gttgcataat tttaaccagc accataggaa gcaatggaag
     1201 ttcgtcaact ttggcttcca ttttgtcaag agcgtccgta gcattcatac agcaccccac
     1261 aaacttgtag cttctcctcc gctgttatcg gctattccca gggtagtctg aatgatggat
     1321 tctcagtttt ggccctttac cgctaatagc cgaaagcaga gattaaaacg cgactcgata
     1381 tgaaaccgtt tttaagatcc tcggatggcg actatttcag ttaagctctt ccaactacca
     1441 atctgaaaca aaaaagtggt tggggaaaag ctaagggtat tcgataaaac ttcatatatc
     1501 gtcttctgga aagagagcat caacaatagc atttaccatc attcgtccca atggattcat
     1561 gttgcaccca aacagcctat ccaacggctg ctggctagaa tcgctattta ctgagaagat
     1621 ttttgggagt aagataactt tgatgagatc cttcccgaaa ttgttagccc tctttctaat
     1681 tggtctgttc ctcgcgttca gctttaatgc ctatgcctgc gtggtgccgg tctttgccaa
     1741 aatgccggtg gctcagactc aaggaagtga ttgcaccatg ccgggggaag agcctgtcac
     1801 ccaattttgt gacggattta aaactctcgc agttcaatgg agtctcgata ctccttcaaa
     1861 cagccagact catgccccct tcattggcga aatggccctg cttcttcctg accttgtccc
     1921 ttcctccaga aaaattatct ttccgcccgg taccggccat gtggccgtgc caaaagacct
     1981 tctcctcctc atctcggttt ttcgtatttg atcccctccc cgtttttccg ttcatcacca
     2041 attccctgaa ttcacgacct cactgatcct ggtctgaaat tttcaggcct gatcgacagg
     2101 atcattttct cttttctgct tctttgggta cgaggcagaa ccggtgagaa gtccatggtc
     2161 gctacataac aagcggagtc aaccatgcac cagatgattt ctagatacgt ggtgataatg
     2221 gcattcggta tggtatggct tggtctgcca ggtccactct cggctcaacc aaccggcacg
     2281 actgaaccgg tattggagct cctgcccctg atccaggaag ccattcaaca aaatcctgag
     2341 atcgcggcga cacaggagca ggtactggcc atgaaggagc gcgcccctca ggtgggagcc
     2401 ctggccgacc ccgaattaaa ggttcagttg tggaatactc ctgactcgct gaatgtcacc
     2461 aaaacacaaa cggttatcta cggggttgcg caacaatttc ctttcccggg attgctctcg
     2521 aagaaagccg aggtggcgac acgggaagcc gaccaggccg aacaacgcat ggccgccaaa
     2581 atccgcgaga ttactgctgc ggtcaagatg gccttttttg agctgttgtt cgcccacaaa
     2641 gccattgatg ttcatcatga acagcaaaaa ttgctccggc agttctttga tattgccaat
     2701 gccaaatacg gagtgggaac ccgaacacag gtagatgtcc tgcgagctca ggtagagctg
     2761 tcaaaaattt cccagcaatt ccccgtcctc aatcaacgac gggaaaccgc tcaagctcat
     2821 ctcaatacca ttctgaatcg tgatccccat gctccgcttg gtatcccgcg agcaccacat
     2881 cctcaaccgc tgacacgctc gcttgaagaa ctccaggagc acgcgcttcg actccgcccg
     2941 gaacttcaag aagctgatct ggcggtgacg cgatttcagt cggccacgca attggccaaa
     3001 ttgcagtact accctcacct tcgggtggaa ctccagcgct ggcaaaatta ccacaccgac
     3061 gacggtttcg gcgggaatgt gatggtgaac attcccttcg cgttctggac caaaccgaag
     3121 tatgacgcag gggtcagaga agctgcggcg caggtcagat ccgcacaagc caagcaacag
     3181 acccttgtca cactcactcg ctttcaggtc aaggatctgg tggtgcaaat tcagtccact
     3241 caagaggttc tggaactcta tcgcaccacc gtccttcccc aggcacagca ggttctccaa
     3301 gccgcccatg cgggttatcg gacggatcgc acggattttt tggatctgat cgatgcggaa
     3361 cgcgcgctca tcacctttcg gcttgaatac atccgggcgc tcgtggaccg ggaacagcaa
     3421 gtcgcccaac ttgaacgcgt gctcggcgcg gacttgtaag aaggagacgc acgatgctca
     3481 cgcacatatc acgaccacgc tggatcatcc ttggaatatt gctctgtatt cttgtggcaa
     3541 gcctgttctt ttttaaagaa tcctggtggc ccctcttcca ttcaacggga acgaccacga
     3601 aggaggcgaa gcatatcgaa caacccatgc aggacatgga agccatgagc agccattctt
     3661 ccatggagag ggaatccagc caggcctatg ccatggtgac tccggcacgg caacaattga
     3721 ttggcgtcac cacagccccg gtaagcatgc gcccgctccg gacggtgatc cgtgccgtgg
     3781 ggaaagtgga ctatgacgaa caacgtctca cccatatcaa tcttcgcatt tcaggttggg
     3841 tcgaagaact ctttgtggat accaccggac aacttgttcg caaaggtcag cccttgttta
     3901 ccctctacag ccaggaactc gtgtcggcgc aggaagaata tctgcttgca atgaaggcca
     3961 acaagcagat tcagaatagc cctcttcttg aaacccggca tcagacggca caaatggtcc
     4021 agtctgcacg cgaccgtctc cggcattgga cattgacgga tgcacagatc gatgacttgg
     4081 cgcgtcgtgg aaagccgcag acctatgtca cgatttattc ccctgccgcc gggtacgtta
     4141 tcgataagaa ggtctttaaa ggcacgttgg tgaaacccga gatgaccctc tatgccattg
     4201 ctgacctttc taccgtatgg gtggaggcgg aagtctttga gtatgaaatg ccctttgtcc
     4261 aagtggggca agagggaatc ctgaccctgg cggcctatcc tggagaaacc tttcgaggag
     4321 aaattacctt tatctatcca tacctcaacc gggaggcccg caccaatacc gttcggctgg
     4381 tgtttaagaa tccgaagctg cgactgaaac cggacatgta tgggaccgtt cagattcagg
     4441 tgaatcgcgg aagcaagctt gctgttcctg aggaagcggt gctcgattcc gggatccgac
     4501 agatcgtcta tgtggtccgg ggagagggca tgtttgaacc acggcaagtc acccttggcc
     4561 cgaaagtagg cggctactat gaagtcgtcg agggtctggc tctggacgag cgggtcgcca
     4621 cctccggcac gtttcttctc gattcggaaa gcaagctcat ggcgagttcg aacatgatgg
     4681 gcgcgctggg gatgggaggt atcaaaatgg agcaggcgca tatgggcaag atggatatgg
     4741 gaatggataa ggataagaat atgccacaaa caaaaacggc caccgccaag aataacgtct
     4801 cgtcggggaa aatggaaaga aaagccgaag ggcttaccct catcctctcc actgacctga
     4861 cgcctgtacg catgggagaa aaccgaatcc agatgaccct aacagacgca gccggcaagc
     4921 cggcctccca tgccaaggtc cggctcacat tcaccatgcc tatgccggga atgattccag
     4981 caaccgttcc aatgacggag gaaaagccgg gcatctatgt ggccaaagtc aatctcggca
     5041 tggcaggaca atgggacctc acgatcaaga ttcaacgttc ggatcaacct gatgtcaaag
     5101 agagattttc cgtgattgct gttggaagcg ggatgccggg gatgccaggc atgtgagcat
     5161 gaaaaacacc caaacctaaa aacaaagtga ggtgagtgat gttgcagaaa tgccaagaaa
     5221 tgttttgtac aagggctgcc gcgagtatgg tcaacgctgt gttactaaca ctaagccttt
     5281 tcgtaacaag ttgggccggt tctgataaca gagtgttgct aatcgaactc gaaaacaaga
     5341 tggcggccga tggaattccg cacgatcaca aggccatagt agacattttt cgaaaagaca
     5401 tacaacgact tgaagcggga acaactctga gtgcggagcg cgcaaccaag cttaaacgaa
     5461 tggctaaaga tcccaagggc gtccagcggc cgcttctcct caatcaggca aataagaaca
     5521 gtcaacaagg cgggatcaac atggagcagg ctcaactgtg gcagatgaat ataggcggca
     5581 aagatacgca aagcacggat atgcgtgtaa aggtgacgga taaccgcagg ctgattccag
     5641 ggacatctaa cgacacttat gcatcgccgg ggaagatgga aacgcttgcc gatggcctcg
     5701 ccatgatttt cacgacggag ccttctcctc cgcgtgtagg agaaaaccgg atggtggcca
     5761 ctgtgacaga cgcgactggc caaccggtat cccgagccag ggtacggctt acatacacca
     5821 tgccgtttca gaacatgact cccaccaccg tgcgattgcg ggaaggaacg aagggagtct
     5881 atgaggccat agtcaatctc ggcctggggg ggctatggga tcttcaggtt ggcattatcc
     5941 gtcccggcca accggagatt caggaaacat attcagtgac agcaggcgga ggagaccttc
     6001 tggaataacc ggaagttcag ctatcgacca tgattgaacg aatcattggc tggagtgcgc
     6061 gtcacggctt tttggtagga atccttaccc tgtttctgat gggttgggga atctgggcgg
     6121 tctatcacac gcccctggat gccctgcccg atctatccga tgtccaagtg atcgtcttga
     6181 cggagtggcc tggccgaagc ccggatttga ttgaggatca aatcacctat cctattgtca
     6241 cctcgatgtt aggcgcccca cgcatcaaat atgttcgagg ccaatcgttt ctgggtttgt
     6301 cctttgtcta cattgtgttt caagacggaa ccgacatgta ttgggcccga agccgggtgg
     6361 tggaatacat gcaggggctg gtgggaaaac tgccggaagg gatctctcca accttaggcc
     6421 cggatgcgac aggggtcggg tgggtctttc aatatgccct tgtggacgaa agcggaaacc
     6481 atagcctggc tgatcttcgc tcgtttcaag attggtattt gcgctattgg ttgcaaagtg
     6541 tgcccggcgt agcggaagtg gcgaccatcg gcggattcgt gaaacagtat caagtccaag
     6601 tcgatcccgt gaaattacag ggatatggta tcggccttcc ccaggtcatt caggctgtcc
     6661 gacgcagtaa caatgaagta ggaggtcggg tcattgaggc cagtgaacgg gaatatatgg
     6721 tacgaggccg cggctacatc caatcgcttg acgatcttcg aaccattccc atcggaatgg
     6781 acggaaaggg gacgcccatt accgtccacg atgtcgcgac cgtgacgatc gggccggatc
     6841 tcagacgtgg cattgcggaa ttaaatggac aaggcgaaac cgtcggtggc atcgtcgtga
     6901 tgcgttatgg cgaaaatgca ctggaagtca ttcaacgggt taaagaaaaa ataacaacca
     6961 tcactccctc cattcctgaa ggcatccgta tcgttccggt gtatgatcgc tcagaattaa
     7021 ttcttcgggc catcgccacg ctcaaagaaa aagtattgga aatcaccatc gtcgtaagcg
     7081 tggttagcct cctcttttta tttcacgttc gctccgcact ggttcccgtc ctcttactac
     7141 cggtcgcgat tctcctgtca tttattgcga tgta
//
LOCUS       DATIAL010000388        19219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_395277, whole genome shotgun sequence.
ACCESSION   DATIAL010000388 DATIAL010000000
VERSION     DATIAL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 19219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19219
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395277"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VLA60_11990"
     CDS             complement(<1..460)
                     /locus_tag="VLA60_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10129.1"
                     /translation="MLQKFHKNRQALIGNHASYSFSQHRHFRRHKGFLFALVLGAGLF
                     IQTLLLPMPAFSESEEVYPEGDQEYFKALLELEKDKEYKKFLMDSVSYAGGRKVEYID
                     YKGVRPTVDGEEGLPGGPGMDAGHVKQQTYEAMRNMDWFRKNNYQNFIRNV"
     gene            complement(872..1177)
                     /locus_tag="VLA60_11995"
     CDS             complement(872..1177)
                     /locus_tag="VLA60_11995"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSF10130.1"
                     /translation="MSFPNKYTLSLTQEAENDIIDILVYTAQQWGEEKLQEYDASLCH
                     ALEVVKDNPLLGYRQSLLPEDHRCFNVRKHVLVYQIKSSHIYVLRILHQRMNLSDHV"
     gene            complement(1161..1445)
                     /locus_tag="VLA60_12000"
     CDS             complement(1161..1445)
                     /locus_tag="VLA60_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10131.1"
                     /translation="MNVRFPDVDEHYIKTKVEAGFYMNETELVRDAVRHMREEDERKT
                     QFLSAIKVGQDQVASGQHRELTPEVLSELRQNATRKIQEGHNPKSDVLPE"
     gene            1686..1928
                     /locus_tag="VLA60_12005"
     CDS             1686..1928
                     /locus_tag="VLA60_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10132.1"
                     /translation="MMTALTPVKRHQATRMNYQPCAMMAPTITNDNFILVDSEGQARR
                     MEVGDSEGHRMPFVVQMIVEHGAMPWYVEFWHWIIG"
     gene            complement(2379..3263)
                     /locus_tag="VLA60_12010"
     CDS             complement(2379..3263)
                     /locus_tag="VLA60_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HSF10133.1"
                     /translation="MRRKIFSFSGVLGIAPKKKGKTEPRPISLIPTDGRIVQRAILNV
                     LQKQSKLQKYLQVETSFGGIEKRGGVPQAIEKALELIAKGASYYIKSDISKFFTKIPV
                     KEVLNILRRELCDDEFIELLDTSIRLEVKVLSGYEKYIHFFDFEEFGTPQGCSLSPLI
                     GNILLFEFDNKMNDSNVSCLRYMDDFIVLATSERAANAAFKKAQSLLVKYGLSAYESG
                     PKVAKAKADKGWTASRFEYLGVEFDGSLRRPSRSNRNRLLDRIKENIKVSQDVIAETK
                     SKEFKKFKDLYEKSYSKP"
     gene            3639..4976
                     /locus_tag="VLA60_12015"
     CDS             3639..4976
                     /locus_tag="VLA60_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSF10134.1"
                     /translation="MTALTLRTLCLLFLLIPATAARGQDVDLKTTVSEAVDTLQHYIR
                     FNTTNPPGDVTEAAAFLQDILQREGIAATRYEAAPGKVNLLARIKGTGKAKAILLLHH
                     MDVVPADASRWDRVKPFGGELKDGQIWGRGAIDMKGTGVLQLYAFLTLARQKVLLDRD
                     IIFMAVNDEEIGGTMGTRYMLDHHYEELDPEYVLDEGGFGSREIFVNGELVFGISVAE
                     KRALWLRVTAHGIAGHGSQPHDQNPNDKLMRALNRLFSAPFPTVPNTALDALESRLGT
                     LAPNQFTNAIRQTTVSLTSLRSGVGDPPKANVIPSLATATLDARLLPGMDAEAFLAEV
                     KSRLGDEIDVEVIHPASQSVVTPHDTPMFRALSNAITRNHPDATVVPMIIPYGTDSRM
                     FRLRGAKCYGILPLVLSTELATSIHGDAERISVDQFETGIRIYYDTLVEVAAR"
     gene            complement(5109..5915)
                     /locus_tag="VLA60_12020"
     CDS             complement(5109..5915)
                     /locus_tag="VLA60_12020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF10135.1"
                     /translation="MSTITVESMDGLFSAPGSGRLRKAATPALRVFKGHDRHEGQAED
                     DPADAVLVERVRRGEETAFKELVEHYHSPLRRVAMTFVNSEAVAEEVVQDTWMAVLEG
                     IHRFEGRSSLKTWIFRILVNRAKTQGVRESRYVEFPGKASDADQGGIDMVDELPVAPG
                     GEGCPGNYSGIISSQTEASPERKLLNKEMVERIYAVIHTLPAMQRQVILLRDVEGVES
                     EHVRRMLGVSESNQRVLLHRARTKVRQALAPYMDESHPHAPKPCALKPCA"
     gene            complement(5940..6197)
                     /locus_tag="VLA60_12025"
     CDS             complement(5940..6197)
                     /locus_tag="VLA60_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF10136.1"
                     /translation="MVRLTVKATQDGTTTVEIEGRIVSEWINVVETECQGLLAQGNNV
                     VLDFSAVNFVGEDGVRMIRRLITQGCRIKNCPSFIQQILFT"
     gene            complement(6407..8836)
                     /locus_tag="VLA60_12030"
     CDS             complement(6407..8836)
                     /locus_tag="VLA60_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10137.1"
                     /translation="MAEVRTTSPFHSEGESVPSAVSAQMSSLGPVPVLQDEVLQRVVK
                     GVEAQTGEKFFDTLVEELARALGVKYAYLSEFNRADGCFYSKAGWSPNGLIPRFQVPV
                     GGPCDVALNHQVVHHADGLADCYPNVQVIKDWGATSYCGLPLINSSGKVAGHLAVLDD
                     KPMPNGDYTASIMRIFAARALAELERVTLDQALRKSDYILRLVDEGTASTSGGEFFQA
                     LACNLAKALGVRYAFVGQLMAEASRVRTLAFWNGEAFIENFEYDLEHSPCQQVLAGEI
                     CHFPERVQQLFPHEKGLVDLGAESYLAIPLLNQAGEVLGHLAALDVRPMPMDARLLPL
                     FRIFGARAGAELERQKIHARLAESENQLRDLFDEAPIAYVNEGLDSRFIRANRAAMQT
                     LGITPDQVEGTYGKSFIPDTPEAQRRLKEAFDSIGRGTDTSGVVLELRRKDNGKPLWI
                     QWWSRPDPGGTYTRTMFLDITDKVLMEQEKARLEAQNTYLREEIQSVHNFEEVIGSSS
                     TLKKVLRNVERVAPTDSTVLVTGETGTGKELIARAIHNLSPRKSKPLVKVNCAAIPAG
                     LIESELFGHEKGAFTGALTKKIGRFELADKGTIFLDEIGELPLDLQAKLLRVLQEGEF
                     ERVGGTQTFKVNVRVIAATNRDLEHLSRTGHYRSDLYYRLNVFPIHLPSLREREGDIP
                     ILAQYFVRKCATNLGKKIDRIPESMMAALRQYVWPGNIRELEHVIERAVILTEGSSLE
                     PIELSTGAGGGGAVSSVMTLEERERRHIMDILEQTGWRVSGKDGAAELLGLKPTTLEA
                     RMKKLGITRKK"
     gene            9397..12066
                     /locus_tag="VLA60_12035"
     CDS             9397..12066
                     /locus_tag="VLA60_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSF10138.1"
                     /translation="MSKRTPHLTIEEERLEESKKRTVHWKRWGPYLSERQWGTVREDY
                     SPNGEAWEDFSHDQARSRAYRWGEDGIGGICDRHQQICFALALWNGKDPILKERLFGL
                     TGNEGNHGEDVKEYYFYLDSTPTHSYMKFLYKYPQHPFPYAQLIEENRKRGKHDWEYE
                     LMDTGVFDDNRYFDVFLEYAKATHEDILIRITAHNRGPDFAELHVLPTIWFRNTWSWT
                     PHAPRPTLNRDEDLGDAQAIHLDHEQYGSRWLLCEGTPDMLFTENDTNTQRLYGVPNA
                     GPYVKDGINDYIVAGAQGTVNPAQVGTKAAVHYHPLIAPGQSITYRLRLTNLPPTEGQ
                     LGEEFETIFPARQEEADEFFAKRLGTAQSEDAQNVQRQAFAGMLWSKQFYHFDVRTWL
                     AGDPTGPPPPAGRKNGRNTDWKHLYNEDIISMPDKWEYPWFAAWDLAFHCVPLALVDP
                     DFAKDQLRLLVREWYMHPNGQIPAYEWAFGDVNPPVHAWATWRVYKIEKRIRGKADRV
                     WLAKIFHKLLLNFTWWVNRKDADGRNIFQGGFLGLDNIGLFDRSAPLPTGGYIDQSDG
                     TSWMGMYCLNMLAIALELARHDPAYGDVASKFFEHFVYISHAINHIGEGNHEASLWDE
                     TDGFYYDMLHFPGGHSQQMKVRSMVGLIPLFAVETLEPEVVDALPGFKRRLLWFIENR
                     PEFRRHVCSTSLPDGSVRRMLSIVDQEQLPRVLKYMLDEEEFLSTYGIRALSKHHQDH
                     PYVLHLNGNEHRVDYEPAESKTGMFGGNSNWRGPIWFPVNFLLIESLQKFHHFFGEHL
                     TVECPTGSGSQQTLWQVAAEISRRLNRIFLHDTSGHRPVNGGIAMFQNDPHWRDYLLF
                     YEYFHGDNGAGLGASHQTGWTGLVTKLLEQSGE"
     gene            12070..14154
                     /locus_tag="VLA60_12040"
     CDS             12070..14154
                     /locus_tag="VLA60_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSF10139.1"
                     /translation="MSPDRRHQHQDIPPPIHPSILPQIVDFGREICGDIWMAERREWL
                     VTNGIGGFASGTVAGSLTRRYHGLLIAALKPPLGRTLLVSKLEETLEYQGKTVQLSTN
                     HWHGGTVAPTGFLFLERFRLEGTTPVWTFAIGNALIEKRLWMEPGENTTYVKYAFTRG
                     SLPIRLNLRAFINHRDYHGITLETLPEFSATPIPHGLQILPAEGSPFILSASGGSVET
                     FNLWYHDYDLPLERERGLPDREHHLHIGTWNLNLKPGEEITWMGSTRLKPSRPAASCF
                     ESRRRHEMALLQSELKAKQSSPSWPDWIQQLTMAADQFIASRPLSANDTGHTIIAGYH
                     WFGDWGRDTMISLPGLTLTTRRFDIARSILSTFAQYADQGMLPNRFPDAGEEPEYNTV
                     DATLWYIEAVRQYVDATDDRAFLNTIFPVLVEMLAWYRKGTRFGIGMDPADHLLHAGE
                     PGVQLTWMDAKIGDWVVTPRIGKPVEINALWYQAWLTLGQLARTMKEPHEEFLQTAKA
                     IHTSFQRFWDEPLHRCYDVLDGPHGHDPAPRPNQLFAVSLPHSPLTYKQQHAVVDYCA
                     QYLYTTYGLRSLGQEDPQYVGQYEGDIRQRDGAYHQGTVWGWLLGPFVLAHFRVYRNP
                     EVALSYLAPMAHHLNDYGLGTLGEIFDGQPPFAPRGCIAQAWTVAEILRAWHLIHTST
                     TKLRTEKHTPQK"
     gene            14872..17388
                     /locus_tag="VLA60_12045"
     CDS             14872..17388
                     /locus_tag="VLA60_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10140.1"
                     /translation="MTLISTLRSFFLRLVINKEDSSQLQASESYTNWCTRFAWERLTV
                     LYLLGLVANPAFIVADVLLHREHLDAILDIRMILELGLLGCFLLVRYQPAAIPPRVFL
                     ILWVLIGNLCIVQMTVVLGGFTAQYYNGLNLVFLAAAVIVPVSWPSHLIAQAGTLVSY
                     YGLNLLNPSVTANIDAAIENSFFLVWTCVALLFSVSLYERLQRAEFHARLFERRARVE
                     LETSNQKLLELDRLKSEFFANISHEIRTPLTLAVGAFKTLLKSALGVEAKDVVQTGLR
                     NASRLLFLINELLDLAKFDSGRTTPNKRCMDFAALISNVVMNFDSSPTRRIHVKGLMQ
                     PVALEADPLQMKKVLYNLLSNAFKFSDPHEGQVWIRLSSKEHSIELEVEDNGIGIPPD
                     QLERIFDRFTQVESSATRRYEGSGIGLALVKEIVMLHGGTIAVESDPGRGSVFTITLP
                     RGNVTADQMLAIQDDEDDRDVLPRPAEGEHKNDESFLPPTEKKDAPLVLVVDDNADMR
                     GYVKKILHRQYQIVVARDGAEALKMAQHLRPALILTDVMMPKMSGQDLLKAVRSDKTL
                     RSTPVIFLTARAGTEARIESLEAGADDYLSKPFDEPELLARVGNLIRARAQERELVQL
                     QKEKLARFLPQNLADMIMSGDHEDFLKGHRSKITVLFIDLRGFTPFTETVAPEEVMAV
                     LREYQAAMGYLISGYGGTLERFAGDGIMVYFNDPQPCPNHAEQAVRMAIAMRQEVHSL
                     EEQWKKRGIPLGVGIGIATGYATIGAIGFEGRTDYAAIGPVTNLASRLCNEAQHGQIL
                     VPEQIAFLLGNLVSTEPVGELALKGIQKPLSVYQVIGLKA"
     gene            complement(17865..18956)
                     /locus_tag="VLA60_12050"
     CDS             complement(17865..18956)
                     /locus_tag="VLA60_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10141.1"
                     /translation="MRRMLVCLFVLVFVIGPSDGMARRVHLSSEQISQLAQIRIILLT
                     SSVRTEEGLFQSEALNALVAKRFQELGFSVVSDSAASHDVEFRVFCEERKRFVGTTPG
                     GGDAELEEAPDRLWNGPACRFRYHLNGADLGWLTEVRAPSSDEKFGFRESGEAFEQSA
                     VFASLLQEVARSEFPLLVMADWGHASRLASLLLDPETQPAQQQIIVQQLAGFSTSTFL
                     PSLLTFIQRQHFPAEAIEALASAGEEGLPHLVALFQDRNQLPTIQASAVRGIGRIYGT
                     TGNRDAFEPMVEYLAGAVDTIASPDDIEYPVLTAVVWSLSKSRSDRVLGLLHRLQAKI
                     WVYHDQSGNMQELREAVSAVSKFLDHVQL"
BASE COUNT         4596 a         5346 c         4711 g         4566 t
ORIGIN      
        1 ccacatttcg aatgaaattt tggtaattgt ttttcctaaa ccaatccatg ttgcgcatgg
       61 cttcatatgt ttgttgtttg acatgaccgg catccattcc aggaccacca ggtagacctt
      121 cttctccatc gacagtgggc ctcacacctt tataatcaat atactccact ttcctgccac
      181 ccgcgtacga aacactatcc atcaagaatt ttttatactc cttgtctttt tcgagttcca
      241 ggagggcttt aaaatattcc tgatcaccct ccggataaac ctcttccgat tcagaaaagg
      301 ccggcatagg taataatagg gtttgaataa ataagccggc ccccaagacc aaagcaaaca
      361 aaaacccctt gtgccgcctg aagtgacggt gttgggaaaa tgaatatgat gcatgattgc
      421 cgatgagtgc ttggcggttc ttgtgaaatt tttgcaacat agtgagttcc ctaatcaaaa
      481 agaatggaaa gaaatttcaa cgcgaatttc ccgcctattg ttgagagaat acctccagat
      541 agtcaattga ctgttccagg atctttcctt ttttattggt tttaattgag aggttcagtt
      601 gggaacgaat aaccaagcac cgtcatggga aaagcgccag aggccttgtt caccttagcg
      661 acggccgaca ttccgatcct ggtgcgactc agggtatgaa gtcgacaccg caaccacaga
      721 aaacttggat gcggaaaaaa ggcgtagctg gcaatcgtaa gggggagtcg tcggagtcaa
      781 tgagaacatt tagaatcata tgcgacgtgt gcccacttga ccagagcctt ctaatgggtg
      841 gccccaaaca ggaaaaactc caagtagggt tttacacatg atcagagaga ttcatgcgtt
      901 gatgcagtat ccgcagcaca taaatatggg aactcttaat ctgataaacc aggacatgct
      961 tcctgacgtt aaaacatcga tggtcttcgg gtaacaagga ttggcggtat ccaagtaggg
     1021 ggttgtcttt tacgacttcc agggcgtgac aaagggaggc atcgtattct tgcagttttt
     1081 cctcgcccca ttgctgggcg gtgtaaacca gaatatcgat aatatcgttt tctgcttctt
     1141 gagtgagaga tagggtgtat ttattcggga aggacatcag acttggggtt gtgtccttcc
     1201 tggattttcc tagttgcgtt ttgacgtagc tcgcttagaa cctctggcgt caattcccga
     1261 tgctgtccgc tggctacctg gtcctgcccg accttgattg cagacagaaa ctgggtcttg
     1321 cgttcgtctt cctcgcgcat atgacgaacc gcatcacgga ccagctcggt ttcgttcata
     1381 taaaacccgg cttcgacctt ggtcttaatg tagtgttcat cgacatcggg aaatctgaca
     1441 ttcatggtat ggccctccat aggatacgat aataatcaca atgcatgtct aatcatagca
     1501 tgattatgtc acatttgaaa gaaaatggga gtactgctgt tgagcagttc cagtcttaga
     1561 gtaaacggta gtgcccccaa ttggcagttg gtttaaatat ctgatttggc aggcaataga
     1621 acgaggagtt aggtcaaaag cagttcatgt gtaagtcata ttgattccac cgctcgataa
     1681 tcaccatgat gacagctctg acacccgtaa agcggcacca ggctacgcga atgaattacc
     1741 agccctgtgc aatgatggcg cccaccatca ccaatgacaa tttcatcctg gtcgatagcg
     1801 agggccaggc ccggcggatg gaagtcggcg actcagaagg gcaccggatg ccgtttgtgg
     1861 ttcaaatgat tgtggaacac ggtgccatgc cctggtacgt cgaattctgg cattggatta
     1921 ttgggtaatt aaccttttaa ccgattgaag aataacagca agcaggggat tctgtcccct
     1981 ggcgacggtt ctcaataccc cgaatcgcga gctttcaaca attcctgatg cattacatct
     2041 catagtaaaa ttgaatgggg catccaagat ctgatcccat tctcttcgag ttaattgtta
     2101 ggacaccatt gattttttag ttgttaaatc ctttgctttt tcatctaacc tagagtcttt
     2161 cctttttaaa tccgtcagaa aataaacacc ttcgattctt ctggccgttt taagaccttg
     2221 ccgaccgcac gttctgtgcc ttttcaatac accaaagaca aaatctctat aggctactaa
     2281 tagatcatct atctctttat ccaaagaacc cataactgca ttgtcattgc aataggaaaa
     2341 ttgatttccc caagagcgaa cgagacaatg tatacctctc acggttttga atacgacttt
     2401 tcgtagagat ccttgaattt tttaaattcc ttcgatttag tttcagcaat aacatcctgg
     2461 cttaccttaa tattttcttt tatgcgatcc aacaatctat ttctgttcga ccgcgacggc
     2521 cttcttaaag acccatcaaa ttccacaccc aaatattcaa aacgtgaggc cgtccaccct
     2581 ttatctgctt tggccttggc aaccttaggc ccggattcat aggctgaaag gccatatttg
     2641 acgagcaacg attgtgcctt tttaaaggct gcattagcgg ccctttctga cgttgcaaga
     2701 acaatgaaat cgtccatata tctaaggcag gagacattcg aatcattcat tttattatca
     2761 aattcaaaaa gaaggatatt tcctattaag ggagacaagc tacatccttg tggcgttcca
     2821 aattcttcaa agtcaaaaaa gtggatatat ttctcatatc ccgagagcac tttgacttcc
     2881 agccgaattg acgtatccag caattcaata aattcatcat cacaaagctc tcttctcaaa
     2941 atattcagaa cttctttaac tggaattttc gtaaaaaatt tacttatatc ggacttaata
     3001 taataagaag ctccttttgc tataagctca agagcttttt cgatagcttg aggaacaccc
     3061 ccacgctttt cgatgccacc aaaactggtt tctacctgca aatacttctg aagtttgctc
     3121 tgtttttgaa gtacgttcag aatagctcgc tgaacgattc gtccatctgt tggaatgaga
     3181 gaaatcgggc ggggttcagt ttttcccttt ttctttggtg caataccaag gacaccagaa
     3241 aaggaaaaaa tttttcttcg caactgggtc cttatttttt tgatattctg aaattcattg
     3301 cttttaaaaa gatctgcttc ctacgagtat ccttggacgc atttggcctg catgacgagg
     3361 catatacatg atcccaggca cgataaaggt tttctgaaga gtagactctt ttgcagagct
     3421 tggaccgtgg cgttgtgagt ggcactgggc ttcaccctcg atggtgcgtc cagatgcttg
     3481 ccggatcaat cctctaaaag tttccagacc aatcagaatt gagtcggata gattatcaac
     3541 ggtattattc aatgccaatg gtcgtgcctg atgatataaa ctgttggacg ctcctcacta
     3601 tgcagaccat taacgtgaac tcccacaggt caaccgttgt gacagctttg actctgagaa
     3661 ctctctgtct gttgtttctg ctgattcccg ccacggcggc tagggggcag gatgttgact
     3721 tgaagacgac cgtcagcgaa gcggtggaca ccttgcaaca ctatattcgg ttcaacacga
     3781 ccaacccgcc gggtgatgtc accgaggccg ccgccttcct tcaggatatt cttcagagag
     3841 aggggatcgc ggccacgcgg tacgaagctg caccgggcaa ggtcaatctt ttagcccgga
     3901 tcaaaggcac aggaaaggcc aaggctattc tgctgctcca ccatatggat gtggtgcccg
     3961 ccgacgcgag ccgctgggat cgggttaagc cgtttggcgg cgaactcaaa gatggccaga
     4021 tctggggccg tggcgcgatc gacatgaaag gcacaggagt ccttcaactt tatgcctttc
     4081 tgacgctggc gcggcagaag gtcctccttg accgcgatat cattttcatg gcggtgaacg
     4141 acgaagagat tgggggaacg atgggaaccc gctacatgct cgaccaccat tatgaggaac
     4201 tcgatcccga gtatgtcctc gacgaggggg gatttggcag tcgcgagatt tttgtgaacg
     4261 gggaactcgt ctttggtatc tccgtggcag agaaacgggc cttgtggctc cgtgtcacgg
     4321 cccacggcat cgccgggcac ggctcacaac cgcatgatca gaatccgaac gacaaattaa
     4381 tgcgtgcgct taacaggctt ttcagtgcgc cgtttccgac cgtccccaat acggctcttg
     4441 acgcacttga gagccggctt ggcacacttg caccaaacca gttcacgaac gccatccggc
     4501 agacgacggt ctcgctcact tctcttcggt caggtgtggg cgatccgccc aaggcgaatg
     4561 tcatcccctc cctggccact gcgactctcg acgcccggtt gctgccgggc atggatgccg
     4621 aagcctttct ggcggaggtg aagtccagac tcggcgatga gatcgacgtt gaggtcattc
     4681 acccggcgag tcagtccgtc gtgacgccgc atgacacccc gatgtttcga gcgttgtcca
     4741 acgccattac acggaaccat cccgacgcga ccgtcgtacc gatgattatt ccttacggaa
     4801 ccgattcgcg gatgtttcgt ctgcgcgggg ccaaatgcta tggcatcctt ccccttgtgc
     4861 tcagtactga actcgccacg tccatccatg gtgacgctga gagaatctcc gtcgaccaat
     4921 tcgagacggg aatccgtatt tattacgaca ccctggttga agtcgcagcc cgctagtgct
     4981 tcggttcccg atccgaaccc cagccctctg atccttgcaa gcgctaagaa accatttttt
     5041 agaaaatccc atacagaatg gggttcagca atccgactcc aagttattcc gcattcctct
     5101 cgcacccttc acgcacaggg cttgagggca cagggttttg gggcatgggg gtgggactca
     5161 tccatgtaag gtgccaaggc ctgacgaact tttgtcctgg cgcgatgtaa cagcacacgc
     5221 tgattggatt cggagacgcc cagcatccgg cggacgtgct cggattcaac cccctccaca
     5281 tcccgcagga ggatcacttg gcgctgcatg gctgggaggg tgtgaatgac ggcatatatc
     5341 cgctcgacca tttccttatt caacaacttc cgttcaggtg acgcttccgt ttgggaggat
     5401 atgattccgg aataatttcc agggcacccc tctccaccag gcgcaaccgg cagttcatcc
     5461 accatatcaa ttcctccttg atcggcatcg gaggcctttc ctggaaactc cacgtaccgg
     5521 ctttcccgca ccccttgcgt tttggcccga ttgaccagaa tgcgaaagat ccacgttttt
     5581 aacgacgagc gcccctcaaa acgatgaatg ccttccagca ccgccatcca ggtgtcttgc
     5641 acgacttctt cggccacggc ctccgaattc acgaaggtca tggcgactcg acgtaatggt
     5701 gaatgatagt gttcgaccaa ttccttaaac gcggtctctt cgccgcgtcg aacgcgttcc
     5761 actaaaaccg catctgcggg gtcgtcctcc gcttgacctt cgtgacggtc gtggcctttg
     5821 aagacccgga gcgccggggt cgcggctttc ctgagccgac ctgaccctgg tgcactgaaa
     5881 agaccatcca tcgattctac tgtgattgta ctcatgggac gctcctccat cggaaccatt
     5941 taggtaaata agatctgttg aataaatgac ggacaatttt tgatacgaca gccttgggtg
     6001 atgagccgcc gaatcatgcg gactccatcc tcccccacaa aattcaccgc tgaaaaatca
     6061 agcaccacat tgttgccttg agccaacagt ccctggcatt cagtttcgac cacgttgatc
     6121 cattccgaca cgattcgtcc ttcgatttcc acagtcgtcg ttccgtcctg ggtggctttg
     6181 accgtgagtc gaaccatcgg tcagggtctc ctgttgttgg atgggaactg cttcatacaa
     6241 agggtattga gcaaccatcg ggccaaaaga aaaccggcca ctcagacggg atccaaatgg
     6301 ccggtttcaa tgaggttttg taatggttga gtgatgagtt cgtgagcggt attcccaata
     6361 tttttcccaa tatttcgtga tggtcacaat atattgggaa tctctcctat ttttttcttg
     6421 ttattcccag cttcttcatt cgggcttcca aggtggtggg tttcaacccc agcagctccg
     6481 cagccccgtc ctttccactg acccgccaac ctgtttgttc aaggatatcc atgatatgcc
     6541 gccgctcacg ctcctccaat gtcatgacag atgaaaccgc ccctcctccc cccgcgccgg
     6601 tcgacaattc gatgggttcc agggaagatc cttccgtgag aatgacggcc cgttcgatga
     6661 catgttccaa ctcgcggata ttcccgggcc aaacatattg tcgcagcgcc gccatcatcg
     6721 attccggaat ccggtcaatt ttttttccta aattcgtggc gcacttccgc acaaaatatt
     6781 gggccaggat cggaatgtcc ccttcccgtt cacgaaggga gggaaggtga atgggaaaca
     6841 cattcagacg atagtacaaa tcggatcgat agtgcccggt gcgggacagg tgctcgaggt
     6901 cccggttggt ggcagcaatg acccggacgt tgaccttaaa ggtttgcgtg ccccctaccc
     6961 gttcaaactc cccctcttgg aggacccgta gcagcttcgc ctgtaaatcc agaggcaatt
     7021 cgccgatttc gtccagaaaa atcgtccctt tgtcggccaa ttcaaaccgt ccgatttttt
     7081 tggtcaacgc tccggtgaat gcgcccttct catgcccgaa gagttcgctc tcgatcaatc
     7141 cggcaggaat ggcagcacaa ttgactttga ccagcggctt ggacttgcga ggactcaggt
     7201 tatgtatggc cctggcgatc aactccttcc cggttccggt ctcccctgtt accagtacgg
     7261 tcgaatcggt gggagccacc cgctcgacgt tgcgcaacac ttttttgaga gtcgatgagg
     7321 atccaatcac ctcttcaaaa ttgtgcacac tttggatttc ctcccgcaga tacgtattct
     7381 gcgcttcaag cctggccttt tcctgttcca tgaggacttt gtcggtgata tccaggaaca
     7441 tggttcgggt ataggtcccg ccgggatcgg gcctggacca ccattgaatc cacaggggct
     7501 taccgttgtc cttgcggcgc aattccaata ccacgccgct ggtatccgtc ccgcggccaa
     7561 tcgagtcaaa tgcctccttc agccgccttt gcgcttcggg agtatcgggt ataaacgatt
     7621 tgccgtacgt cccttccacc tgatcaggcg tgatgcccag ggtctgcatc gccgcacgat
     7681 tcgcccggat aaacctggaa tccagtcctt cgttcacata ggcaatcggt gcctcatcga
     7741 acagatcacg caattggttt tcactttccg caagcctggc atggattttc tggcgttcca
     7801 attccgctcc ggccctggcg ccgaaaattc gaaagagcgg aagcagcctg gcgtccatcg
     7861 gcatgggtcg cacatccagc gcggccaggt gccccaacac ttcaccggcc tggttgagca
     7921 acggaatggc cagataactt tctgcgccca gatcgacgag tcctttttca tggggaaaca
     7981 gttgttgaac gcgttcaggg aaatggcaga tttccccggc aagcacttgt tggcaggggc
     8041 tatgttccag atcatattca aagttttcaa tgaaggcctc accgttccaa aaggccagag
     8101 tgcgcacccg gctcgcctcg gccatgagtt gccccacaaa ggcataccgg acacccagcg
     8161 cctttgccag gttgcaagcc agtgcctgga aaaattctcc tccggaggtc gaagcggtgc
     8221 cttcatcgac gagccgcagg atataatcac ttttcctgag ggcttggtcc aacgtcaccc
     8281 gttcaagttc cgccaacgcc cgagcggcga aaatacgcat gatggaagcc gtatagtctc
     8341 cattgggcat cggcttatca tcgagcaccg ccaaatgccc ggccaccttt cctgaggaat
     8401 tgatgagggg gaggccgcaa taactggtgg ctccccagtc tttaataact tggacattgg
     8461 gataacaatc ggccaagccg tcggcatgat ggactacctg atgattcaac gccacgtcac
     8521 aggggccacc aaccggcacc tggaaacgag gaatcaatcc gttcggcgac catccggctt
     8581 ttgaataaaa acatccgtcc gctcgattga attccgacag gtaggcatat ttcactccca
     8641 gcgccctggc cagctcctcg accaacgtgt caaaaaattt ctctcccgtt tgcgcctcta
     8701 cccctttgac tactcgctgg agcacttcgt cctggagcac agggacggga ccaagggaag
     8761 acatctgagc ggaaacggcc gaagggacgg actctccctc agagtgaaat ggagacgtgg
     8821 tgcgtacctc agccattaca tcctcctctg aaatggcaat gcgtgagaaa aacctggccg
     8881 tcttgagtag gaccctaccg gcgctattat ctccaaggag ccgggtgaaa ggaaatagat
     8941 cggttcccgg attatcccga cgacttccta aacgttcccc tgaccgactt ctcctgggcc
     9001 cctttcattc ggttccggaa ccggccaaag aaaattgcac ggcatggagg gattttccgg
     9061 aggttgatcc cctaccatcc tggccgaaga tctaggacag tttacggtta tttcgtgata
     9121 cactaaaatt gtggaacgga gcaggaggct cgactatact cgttggaccg tccaacgacc
     9181 ttttctccgg cggacctgtt tggctaacaa cccttcactc atctttgtga cacattgcca
     9241 acaactcgga acatgtttca ggtatcggag cctgtgagca tcccgacgtt gaacccggga
     9301 tttttacgtg ggccgatagt tcattccccg aacggatatg tcctgcaaat cgtccggcga
     9361 taacccgtca agcatcgacc ataggaaccc ccttccatgt ccaaacgcac acctcacctg
     9421 accatcgaag aagaacgctt agaagagtcc aaaaagcgga cggtgcattg gaaacggtgg
     9481 ggaccctatc tgagtgaacg ccaatggggc acggtccgtg aggattacag tccgaatgga
     9541 gaagcctggg aagatttctc ccatgaccag gcccgctcac gggcctaccg gtggggcgaa
     9601 gatggcatcg gcggcatctg cgaccgccac cagcagattt gttttgcgct ggcgctctgg
     9661 aacggaaaag atcccattct gaaagaacgt ttgtttggct tgacagggaa cgaaggcaat
     9721 catggggaag acgtcaaaga atattacttt tacctggact ctacacccac gcattcctac
     9781 atgaagtttt tgtataaata tccccaacac ccctttccct atgcccaatt gatcgaagaa
     9841 aaccggaagc ggggcaaaca cgattgggaa tatgagttga tggacacggg tgtgtttgat
     9901 gacaaccggt atttcgacgt gtttctcgaa tatgccaaag cgacacacga agacatcctg
     9961 atccggatta ccgcccataa ccgggggcct gattttgcgg aacttcatgt cttgccgacc
    10021 atctggtttc gcaacacctg gtcctggacg ccgcatgcgc cacgtcctac tctcaatcgg
    10081 gatgaagacc ttggcgatgc gcaggccatc cacctggacc atgaacaata cgggtcgcgg
    10141 tggttgttat gcgaaggcac tccggacatg ctctttaccg aaaatgacac caacacgcaa
    10201 cggctctacg gagtgcccaa cgccggcccc tatgtcaaag acgggatcaa tgactatatc
    10261 gtcgccggcg cacaagggac cgtaaatccc gcacaagtgg gaacgaaggc tgcggtccat
    10321 tatcacccgt tgattgcccc aggacaaagc atcacctatc gcctgcgatt gaccaacctc
    10381 cctccgaccg aaggccaatt gggcgaagag tttgaaacca ttttccctgc gcgccaagag
    10441 gaggcggatg aattttttgc caaacgactc gggactgcgc aatccgagga cgcgcaaaat
    10501 gttcagcgtc aggcatttgc cgggatgctg tggagcaaac agttttatca ttttgacgtg
    10561 cgcacctggc tggccggaga ccccacagga ccgccaccgc ccgcagggcg aaaaaatggc
    10621 cggaacacgg attggaagca cctctataat gaggacatca tttccatgcc cgacaaatgg
    10681 gaataccctt ggtttgcggc ctgggacctt gcctttcatt gcgtgccatt agccctggtg
    10741 gacccggact tcgcaaaaga tcagcttcgg ctattggtca gagaatggta catgcatccc
    10801 aacggacaaa ttccggcgta tgagtgggcg ttcggcgatg tgaatccgcc cgtccacgcc
    10861 tgggccacct ggcgggtgta caaaattgaa aaacgcatca ggggcaaagc cgaccgggta
    10921 tggttggcca agattttcca caaactcctg ctgaatttta cctggtgggt gaaccggaaa
    10981 gatgccgatg gccgaaacat ttttcaggga gggttcctcg gactggataa tatcggcctc
    11041 ttcgaccggt ccgcgccgct tccgaccggc ggatacatcg atcaatccga cggcaccagc
    11101 tggatgggca tgtactgttt gaacatgctg gccattgccc tggaactggc acgacacgat
    11161 ccggcctacg gcgatgtggc cagcaagttt tttgaacatt ttgtctacat ctcgcacgcc
    11221 atcaatcaca tcggcgaggg caatcatgaa gcctccctat gggatgagac cgacggattc
    11281 tattatgaca tgttgcattt cccgggcggg cattctcaac aaatgaaggt ccggtccatg
    11341 gtgggactca tcccgctctt tgccgttgag acgctggaac cggaagtggt agatgccctg
    11401 ccgggcttta aacggcgcct gttgtggttt atcgaaaacc gtccggaatt ccggcgtcat
    11461 gtctgctcaa ccagcctgcc ggatggcagt gttcgccgga tgctctccat tgtagatcag
    11521 gagcaactcc cccgcgtcct gaagtacatg ctggatgaag aggaatttct ctccacatat
    11581 ggcattcgag ccttatccaa acaccatcaa gaccatccct atgtgctcca tctgaatgga
    11641 aatgaacatc gcgtggatta tgaaccggcc gaatccaaaa cagggatgtt cggcggcaac
    11701 tccaattggc gaggaccgat ttggtttccg gtgaattttc tgctcatcga atcgttacag
    11761 aaatttcacc atttcttcgg ggaacatctg accgttgaat gcccaaccgg atcaggatcg
    11821 caacagacat tgtggcaggt cgcggcggaa atttcccgtc gcctcaatcg gatcttcctt
    11881 catgatacct cgggccatcg accggtcaac gggggaatag ccatgtttca gaacgatccg
    11941 cattggagag attatctgtt attttacgaa tattttcacg gggacaacgg ggcgggatta
    12001 ggcgcaagcc accaaacggg atggaccgga cttgtgacta aactcttaga acaatcagga
    12061 gaataggata tgtctcccga ccggaggcac caacaccagg acatcccacc tcccatacac
    12121 ccgtctatcc tgccacagat cgtggatttt ggcagggaga tttgtggaga tatctggatg
    12181 gcggaacgcc gggaatggct cgtcaccaac ggtatcggag ggttcgcctc cggcaccgtg
    12241 gcgggttccc tgacccgacg ctatcacggc ctgctcatcg ccgccctcaa gccacccctc
    12301 ggacgcacct tgttagtcag caaactggaa gaaaccttag agtaccaagg gaagaccgtt
    12361 cagctctcca ccaaccattg gcatggcgga actgtggcgc caacgggatt tttattcctg
    12421 gagcgctttc gcttggaagg cacgacaccg gtctggacgt ttgccatcgg caacgcatta
    12481 atagaaaagc gcctatggat ggaaccgggt gagaacacca cctacgtgaa gtacgctttc
    12541 accagaggat cgcttccgat tcgcctcaat ctgcgagctt tcatcaatca ccgcgactac
    12601 catggcatca cgctggagac cctaccggaa ttttcagcca ctcccatccc gcacggactc
    12661 cagattctcc ccgcagaagg ttctccgttt atcctatccg catcgggagg atcggttgaa
    12721 actttcaatt tgtggtacca cgattacgat ctgccactcg aacgcgaacg gggacttccc
    12781 gatcgcgaac atcatctgca catcggcacg tggaacctca acttgaaacc gggcgaagag
    12841 atcacctgga tgggcagcac ccgtcttaag ccgtccagac ccgcggcttc gtgtttcgaa
    12901 tcacgccgac ggcatgaaat ggcattgctc caatcagaac tcaaggcaaa acagtcatcc
    12961 ccgagttggc cggactggat ccaacagctc acgatggccg cagaccagtt catcgcctct
    13021 cgtcccctct cagccaacga caccggccat acgatcatcg ccggttatca ctggtttggt
    13081 gattggggcc gggacacgat gatcagcctg ccgggactca ccctgaccac caggcgcttt
    13141 gacatcgctc ggagcattct ttccacattt gcgcaatatg ccgaccaagg tatgcttccc
    13201 aaccgattcc ccgacgcagg ggaggaaccg gaatacaaca ccgttgatgc gacgctctgg
    13261 tacatcgagg ccgtccgcca atatgtggac gccaccgatg atcgcgcctt tctcaacacg
    13321 atctttcccg tgcttgtcga gatgctggcg tggtaccgca agggcaccag gtttggaatc
    13381 ggtatggatc ctgccgatca cttactccat gccggcgagc cgggcgtaca gctcacctgg
    13441 atggatgcca agatcggaga ctgggtcgta actccgcgga tcggcaaacc ggtcgagatc
    13501 aatgcgctgt ggtaccaagc ctggctcacc ctgggccaat tggccaggac catgaaagag
    13561 ccgcacgaag aatttctcca aacagccaag gccatccaca catcctttca acggttctgg
    13621 gacgaaccct tacaccggtg ttatgacgtg ctggacggac cccatggcca tgatccggcc
    13681 ccacgcccca atcaattgtt cgccgtgtcg ttgccacaca gtccgctcac ctacaaacag
    13741 caacatgcgg tcgtggatta ttgcgcccaa tatctctaca caacgtatgg gttacgcagc
    13801 ctggggcagg aggatcccca atacgtagga caatatgaag gagatatccg gcagagggat
    13861 ggcgcctatc atcaaggcac cgtctggggc tggctactcg ggccttttgt gctggcacac
    13921 tttcgggtct atcgcaatcc ggaagttgct ctgtcttacc ttgccccgat ggcccaccat
    13981 ctcaacgatt acggactggg aacgctgggc gaaatttttg acggacaacc gccattcgct
    14041 cctcgtggat gcatcgccca agcctggacc gttgccgaaa tcctccgcgc ctggcatctc
    14101 attcacacgt ccaccactaa gcttagaaca gaaaaacaca ctccccaaaa ataaagtgct
    14161 ttggtgtcaa ttaagacttg tcattctgag gccaggccga agaatctgtg cccagcctga
    14221 agatgtaagg actcaaaggg atcctttctt tttccgcaga tgcctctctc aactcttgtt
    14281 ttctctccca catcctccaa tgttacccaa ttaataggga cttaaaggat ttgaagataa
    14341 tggacctttt gtctatagtc ttttaaacaa ctggctggag gcactgtcat ggtgacggtc
    14401 ccatttcgct ggatcgtggc ggaccagatg caagaaggac ggccagccgc caatacaatc
    14461 gcgttcgatt tccaaaacac cggcaatggc tttattcaga ggcctctccg taacctacaa
    14521 aataatctca ttcctccaaa ctacccggac cgccccttgg cggccagaaa ccaggcgttc
    14581 caaatgcctt cgttcctggt cggtcgagtg aatgggggcg tcccctcatg ctcgcatggg
    14641 caacacctcc atattcagat agcagaaaaa ggaatacttc atacatcacg acaatgatca
    14701 ttccctacat tcgtacagtt ttgtggaata gttatggttc ttccttccct atcgattgac
    14761 cttttttttt cgaatcgaaa cgtatgggcg aaccgcgcat gccttgtacg agcagagcag
    14821 taatcaatgg atagactgtc aaaacctacc tagatcattc cgaggttcac gatgaccctc
    14881 atctccacgt tacgtagttt tttcttaaga ctcgtcataa acaaggaaga cagttcgcag
    14941 ctccaagcat ctgaatccta cacaaattgg tgcacccgct ttgcctggga acgtctcacc
    15001 gttctctatc ttttgggatt ggtcgccaac cctgcgttta ttgtagccga cgttctcctc
    15061 catcgagagc atctcgatgc aatcctcgac attcgcatga ttttggagtt agggctcctc
    15121 ggttgttttc tgctggtaag ataccaacct gctgcaatcc cgccacgtgt gttcctaatc
    15181 ctttgggtat tgattggcaa tctgtgcatc gttcagatga ccgtcgtgct tgggggattc
    15241 acggcccagt attacaacgg gctcaatctg gtgttcctgg cggcagcggt cattgtgccc
    15301 gtgtcctggc caagtcatct gatcgctcaa gccggaacgc tcgtgtcgta ttacgggctg
    15361 aatcttctca atccttctgt gacggctaat atcgatgcag ccattgagaa ctcgtttttc
    15421 ctcgtatgga cctgcgtcgc cttactcttc tcggtttccc tctacgaacg actgcagcgg
    15481 gccgaatttc acgcacggct ctttgagcgc agggcaaggg ttgaattgga gacgtccaac
    15541 caaaaacttc tcgaactgga tcggttgaaa agtgagttct tcgccaacat cagccatgaa
    15601 attcgcacgc cgctcactct ggcggtgggt gcatttaaga ctctcttgaa atctgcactc
    15661 ggcgtagaag ccaaagatgt ggtccagacc ggcttacgga atgcctccag gcttcttttc
    15721 ctcatcaatg agttgctgga cctggctaaa ttcgacagcg gtcgcaccac tccgaacaaa
    15781 cggtgtatgg atttcgctgc gctcataagc aatgtggtta tgaacttcga ttccagcccg
    15841 actcgtcgca tccatgtgaa aggcctgatg caaccggtgg ccttggaggc cgatccactc
    15901 caaatgaaga aagtgttgta taacctgctc tccaacgcct tcaagttcag cgatccccat
    15961 gagggccagg tgtggattcg tctctcctcc aaggaacatt ccattgaact ggaggttgaa
    16021 gacaacggga tcggcattcc gcccgatcaa ttagagcgaa tcttcgaccg attcactcaa
    16081 gtggaaagca gtgccacgag gcggtatgaa ggcagcggga tcggattggc cttggtgaag
    16141 gaaatcgtca tgctgcatgg gggaacaatt gcggtggaaa gcgaccccgg ccgtggttcc
    16201 gtatttacca tcacgttgcc tcgcgggaac gtgaccgcgg accagatgct tgccattcag
    16261 gacgacgagg atgaccggga cgttcttcct agaccagccg aaggcgaaca caaaaacgat
    16321 gagtccttcc tcccccccac tgaaaaaaag gatgccccat tggtgttggt ggtcgatgac
    16381 aatgccgata tgcgcgggta cgtcaaaaaa atattgcaca gacaatatca gatcgtagta
    16441 gccagggacg gggcggaagc cttaaagatg gctcaacact tgcgcccggc attgatcctg
    16501 acggatgtca tgatgccgaa aatgagcgga caggaccttt tgaaagccgt tcgaagtgat
    16561 aagactcttc gatcgacccc cgtgattttc ttaacagccc gcgctgggac cgaagcacgt
    16621 attgaaagtc tggaagccgg agccgacgat tatctgtcca aacccttcga cgaaccggaa
    16681 ctcctggctc gcgtcggcaa cctgattcga gcacgcgcac aagaacggga actcgtacaa
    16741 ttacagaaag aaaaattagc caggttttta ccccagaatc tggccgacat gatcatgtcg
    16801 ggtgaccacg aggatttcct gaaagggcat cgcagcaaaa tcaccgtgct ctttatcgat
    16861 cttcgaggat tcacaccctt tactgaaacg gtggctcccg aagaggtgat ggccgtcctc
    16921 cgtgagtacc aggcggcgat gggttacctg atctccggat atggcggaac gcttgagcga
    16981 tttgccggcg acggcatcat ggtctatttt aacgacccgc agccctgtcc caatcacgcc
    17041 gaacaagcgg tgcgcatggc aatcgccatg cgacaagaag tccattcgtt agaagagcaa
    17101 tggaagaaac gcggcattcc cctcggagta ggcatcggta ttgcgaccgg atatgcgacg
    17161 atcggtgcga tcgggtttga aggacgaacg gactatgccg cgatcggccc tgtcaccaat
    17221 ctggcatcca gactctgcaa cgaagcccaa cacggccaaa tcctggtccc ggaacagatc
    17281 gccttccttc tcggaaatct ggtttcgacc gaaccggtcg gggaactcgc actgaaaggc
    17341 attcaaaagc ctctttccgt ctatcaagtg atcggattaa aagcctagtt gttattctgc
    17401 ggccccgctg aagaatctga ggccagccct aaaacgtcct cgtctcccag aacgccttct
    17461 tttcctatcg gctcaccatt ggcaaaaatt ccccctccgt catccccgac atctgtcatc
    17521 ggggatccat cttccctccg acattcctcc ccccgcattg gcatcctgat ccattcgact
    17581 tcgctcagga caagggctcc gcgaagaatc tgtgcccagc ctgaagaccc cacaactccc
    17641 cgagatcatc agatttcttc aggcagtccc ccacccgcct tgtcattctg agcccgcggc
    17701 gaagaatctg tgcccagcct gaagatgcct tcgtttccca gaacctcttc tccgtcatcc
    17761 cgacattcgt tataaggtat ccatctattg aatttgttct tcctgctcgc cttttccagg
    17821 aatttcttta ttccataatt ccgtattttc cacacctcat cacctcagag ttgtacgtga
    17881 tcaagaaatt tggatacggc actgaccgct tcccgaagct cctgcatgtt cccggactga
    17941 tcatgataga cccagatttt cgcttgcaga cggtgcagga gcccaagcac ccggtctgac
    18001 cgacttttgc tcagggacca gaccaccgcc gtaagcactg gatactcaat atcatcaggt
    18061 gaggcgatcg tgtccaccgc gcctgccaga tactccacca tcggttcaaa cgcgtcccga
    18121 tttcccgttg tgccataaat ccggccaatc cctctgacag cggaagcctg aatcgtaggc
    18181 aactgattcc gatcctggaa caacgccaca agatgaggca acccttcctc ccccgcggat
    18241 gccagggcct caatcgcttc cgccggaaaa tgctgccgtt gaataaaagt cagcaggcta
    18301 ggaagaaatg tagatgtgga aaaacctgcc aactgttgaa caatgatttg ttgttgagcg
    18361 ggttgggttt caggatccaa caggagcgag gccaatcgtg aggcatggcc ccagtcagcc
    18421 atgaccagca acgggaactc cgatcgtgcc acctcttgaa gcaacgaggc aaacacggca
    18481 ctctgttcaa aggcttcccc ggactcacgg aacccgaact tctcatcgga agaaggagcc
    18541 cgtacctccg tgagccaacc caggtctgca ccattcaaat gatatcggaa ccggcaggcc
    18601 ggaccattcc atagacgatc cggggcctcc tccaattccg catccccacc cccaggtgtg
    18661 gttcccacaa accgtttccg ctcctcacaa aacacgcgaa actctacatc atgcgacgca
    18721 gcggaatcgc tcacgaccga aaaacccagt tcttgaaacc gtttcgcaac cagagcattc
    18781 agtgcctctg actggaagag cccctcctcc gtcctgacgc tggaagtaag aaggatgata
    18841 cggatctgag ctaactgcga gatctgttca gaggaaagat gcacccgcct ggccattccg
    18901 tcggaaggtc cgatgacgaa gaccagcaca aacaggcaaa cgagcattcg acgcatggat
    18961 gctctcctgg agaaaggttg gcggggttag aaccccaggc ttgcaacatc ttcgttccag
    19021 gggaaccggc ttatccggga cacacatcgt gacctccccg ttgaccgtag gcaacaatcc
    19081 ggtgaccaga aattcggaca tcatcggacc gatcagcttg ggtgccaaac tgacaaccgc
    19141 cacaataagc gttctttaca gctcctcagt tttggacaga tgcgtgttct gcgcacacga
    19201 ttgctttatg ccgacttcc
//
LOCUS       DATIAL010000389         4076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1039003, whole genome shotgun sequence.
ACCESSION   DATIAL010000389 DATIAL010000000
VERSION     DATIAL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039003"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VLA60_12055"
     CDS             <1..618
                     /locus_tag="VLA60_12055"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HSF10142.1"
                     /translation="ANYPLHIGITEAGTAMTGAVKSAIGLGWLLSQGIGDTLRVSLAA
                     DPVEEVKVGFEILKSLELRHRGVNVIACPTCGRVEIDVVKMANELEHRLGHITTPITV
                     SVLGCVVNGIGEGKEADIGIAGGQGVGILFKKGKLYKRVPSEDLLHTLIEEVELMAKE
                     QGGQGPSVNVHETLDGVPIHQTPMEDLSLSPIRSVSKELPVLPRQ"
     gene            708..783
                     /locus_tag="VLA60_12060"
     tRNA            708..783
                     /locus_tag="VLA60_12060"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:741..743,aa:Cys,seq:gca)
     gene            887..1903
                     /gene="hpnH"
                     /locus_tag="VLA60_12065"
     CDS             887..1903
                     /gene="hpnH"
                     /locus_tag="VLA60_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HSF10143.1"
                     /translation="MSVPVSQMYTVAHYALSQHLRGVKRYPLVLMLEPLFRCNLECAG
                     CGKIQYPDHILNRRLTPEQCWAAADECGAPIVSIPGGEPLIHPEMPAIAEGLVKRKKY
                     VYLCTNAILLERKLQDYTPSKYLTFSIHMDGLKEEHDHAVCRDGVYDVAVKAIKAALA
                     KGFRVTTNTTLFNDAAPARVRRFFDEMMELGVEGMMISPGYSYEKAPDQHSFLSRDRT
                     HSLFAQLLSQRKRAWQFNQSPLFLEFLMGKREYECTPWGNPTYNIFGWQRPCYLLQDG
                     YVSTFRELMEETEWERYGTGRHEQCRDCMVHCGYEASAVKDTFSSWSGFFGTVRATLF
                     PNAV"
     gene            1994..2341
                     /locus_tag="VLA60_12070"
     CDS             1994..2341
                     /locus_tag="VLA60_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10144.1"
                     /translation="MGLDASSMDTPQSWEGRHLQPSNEEELHEAMDRVFDYRGDVTIH
                     LQTGEQVIGFVFDRQEEAPEPYIKLFLPGDQQPRMVLYQEVAEIIFSGQDTAFGRSWE
                     DWAQKWKKPEASS"
     gene            3152..3712
                     /locus_tag="VLA60_12075"
     CDS             3152..3712
                     /locus_tag="VLA60_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HSF10145.1"
                     /translation="MNEFQTVKSLLVSGLAVMGLLMSACGGGEGPPQPPPPAPPEYAD
                     KHMPAGYWNNPEILKEGEAIFTGQQNIDVNCASCHGKDGKPVKAGARDFRRTEQMQLY
                     SDAVWFWRISEGVTGTKMKAWKSKLSEDAIWKVIAYEATFGLKGMEYDVAKKQWVPAG
                     TAGSAPGGDAPSAEAPAGGEAEKAAE"
     gene            3751..>4076
                     /locus_tag="VLA60_12080"
     CDS             3751..>4076
                     /locus_tag="VLA60_12080"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSF10146.1"
                     /translation="MKTLNRSLWIVLALGLSGGGVAFGQVPDAPLVDFPYSGNRTAVW
                     VVAQLHILFAAFILGAPIFAVVAEWLGYKNNDPKYDRLAKEVIKVTVILYSMTALTGG
                     LFIFVLL"
BASE COUNT         1030 a          806 c         1128 g         1112 t
ORIGIN      
        1 gccaattacc cattgcatat cggcattacc gaagcaggaa ctgcgatgac cggtgcggtg
       61 aaatctgcta ttgggttagg ttggctgctt tctcagggta ttggcgatac ccttcgggtc
      121 tctttagcgg ccgatccggt ggaagaagtc aaagttggat ttgagattct gaaatcgctt
      181 gagcttcgtc atcgtggtgt caatgtgatt gcttgtccga catgtgggcg agtggaaatt
      241 gatgtggtga aaatggctaa cgaattagag catcgactgg gacatatcac cacgccaatt
      301 actgtatccg tcttgggttg tgtggtgaat ggaattggcg aagggaaaga agctgatatt
      361 ggaattgccg gaggtcaagg cgtaggaatt ttgtttaaaa aagggaaatt gtataaacgt
      421 gtgccatctg aagaccttct tcatactttg attgaagaag tggagttgat ggctaaagag
      481 cagggtggtc aaggccctag cgtcaatgtt catgagacgc ttgatggcgt tcccattcat
      541 caaaccccaa tggaagatct ttccttaagc ccgatacgat ctgtctccaa agaacttccc
      601 gtgttgcctc gtcagtaatg attccagcct tttgaggact aaagagcgag aaagccagat
      661 tttctcttgt acaagagaaa atagacttct tataatgagc tcggaaaggc gccgtaccca
      721 agtggctaag ggagcagtct gcaaaactgc gattcagcgg ttcgaacccg ctcggcgcct
      781 ccatttattt tatcctgttt aacccaggca ttggttttag gatttatgct gggagtagga
      841 gtgaattctt gaaatatggt gaaaatctag agggagtaaa ataacaatgt cggtacctgt
      901 gtctcaaatg tataccgtag ctcattatgc cctctcgcag cacctacggg gtgtgaagcg
      961 ataccctctt gtgctcatgc tagagccgct ctttcggtgt aatctggaat gtgcagggtg
     1021 tgggaaaatc cagtacccgg atcatatttt gaatcggcgt ctgacgcctg agcaatgttg
     1081 ggctgccgct gatgaatgtg gtgcacctat tgtgagtatt cctggaggag agcccctgat
     1141 tcatccggaa atgccagcca ttgccgaagg gcttgtgaag cgaaagaaat atgtttacct
     1201 ctgtacaaat gccattctgc ttgaacgaaa attacaggac tataccccct cgaaatacct
     1261 cacatttagc atccatatgg atggtctcaa ggaagagcat gaccatgccg tttgtcgaga
     1321 tggtgtctat gacgttgccg tgaaagccat taaagccgct ttggccaaag gttttcgggt
     1381 gacgaccaat acgacattat ttaatgatgc ggcgcctgcc cgtgtcaggc gattttttga
     1441 tgaaatgatg gagctgggtg tggaaggcat gatgatttct cctgggtata gctatgaaaa
     1501 ggctccagat cagcatagtt ttttgagtcg tgatcggaca cattcgttat ttgcgcaatt
     1561 gctgtctcaa cgaaagcgcg cttggcagtt caaccagtct ccgttattcc ttgaatttct
     1621 gatgggaaaa cgggagtatg aatgcacccc ttgggggaat cccacgtaca atatttttgg
     1681 atggcaacgc ccctgttatt tattacagga tgggtacgta tccacttttc gagaattaat
     1741 ggaagaaacg gagtgggagc gttatggaac gggacgacat gaacaatgtc gggattgtat
     1801 ggtgcattgt gggtatgaag cctctgctgt gaaagatacc ttcagctcat ggagtgggtt
     1861 ttttgggacc gtcagggcga ccctgtttcc caatgcggtg taaggcttct tctctttgtt
     1921 tgttttttcc ccttcctcaa ctctgggtcc tctgaaaatt gtctcaacct ttagctgtaa
     1981 gtaaggggtc agcatgggtt tagatgcatc ttcaatggat actccacagt catgggaagg
     2041 tcggcatctg cagccttcga atgaggaaga attgcacgaa gccatggatc gtgtatttga
     2101 ttatcgaggc gatgtgacga ttcacctcca aacgggggag caggttatag gatttgtatt
     2161 tgatcgtcag gaggaggctc ctgagccgta tattaagcta ttcctaccag gagatcagca
     2221 gcctcgtatg gttttatacc aagaggtggc tgaaatcatt ttctcagggc aagatacggc
     2281 gtttggtcga tcatgggagg actgggcgca aaagtggaag aagcccgagg caagctctta
     2341 acgacaggat tcccgcaagc atttttatcc cttcaatgtt ctgaagcttc catataatcc
     2401 aaaacaggtt ttccttgtaa tcggactcta ggcgtgatga gaaatttatt tctctctcga
     2461 ctcatgcact cgtatgttta ttctctgaaa acacaggaat ggtgagtggg aaacagcacg
     2521 caagaacgga agaaataatt gacggttggc ttctgactgt gatagaagtc ctgaactttt
     2581 ttgggcccag gagacctatg tagcccgaag atttctgaat gggataaatg gggttggcag
     2641 agtattttta ttgaaggaaa tgtgaaggct tgcgaaaact gcagaatgta gggtgtacac
     2701 aaagggtgca atcacgtcat ttcgttctag ctttcatatt ttctaatttg gtcgcttttc
     2761 ccagtccgtg tgttgtatgc ccttcatgat gtagaccatc gctttatgat tgagggtcgg
     2821 attattggaa atggcgggga tggctttgag tcggctgtcc cttccgtgaa ggatgcttga
     2881 gcagatgttt cgaggaatgg aaaagcagat aggatggatt tagcaaagtc gttttccact
     2941 tggacagtgc aaaccagggg catcgctggg ttattcaatc aggagcgttt gagtcgacac
     3001 ggtgtgtgga gattgacctg gctagtccaa ggccagattg aatcattgcg gcaaactttg
     3061 ggcgcccatg ccctgtcgtt ccgtagttac taagattttg tgaggaatgt ggaatttgaa
     3121 aattcaagaa tcaggtaaac gagggggaac aatgaacgag tttcaaacag taaaaagcct
     3181 tttagtcagt ggtttagccg ttatgggcct tttgatgtca gcatgtggtg gtggcgaagg
     3241 tcccccgcaa ccgcctcctc ctgctccacc ggaatatgcg gataagcata tgccagccgg
     3301 ctattggaat aatccagaaa ttttgaaaga gggagaggcc atctttactg gacaacagaa
     3361 cattgacgtg aattgtgcga gttgccacgg gaaagatggg aaacccgtta aggccggtgc
     3421 ccgggacttc aggcgaacag agcaaatgca gctatattcg gatgccgtct ggttttggcg
     3481 aatttctgag ggagtgactg ggacaaaaat gaaggcgtgg aagagcaagc tttcagagga
     3541 cgccatttgg aaagtcattg cgtatgaagc gacgtttggg ctcaagggga tggaatatga
     3601 tgttgcgaaa aagcaatggg ttcctgcggg aacggctggg tcagcacctg gaggtgatgc
     3661 tccatccgcc gaagctccag ctggtggaga ggctgaaaaa gcggcagagt aatccaggaa
     3721 ttgaatgaaa aataaggagg gcggggaccc ttgaaaacac tgaatcgaag tctatggata
     3781 gttctagcgt tgggcctaag tggggggggg gttgcatttg gacaggtacc tgacgctccc
     3841 cttgtggatt ttccttattc gggaaatcga acggccgtgt gggtcgtcgc tcaacttcac
     3901 atcttatttg cggcgtttat tttaggcgct ccgatatttg ccgtagtggc tgagtggctt
     3961 gggtataaga ataacgatcc caagtatgac cgtctggcca aggaagtcat taaggttacg
     4021 gtcatcttat acagtatgac tgccttaacc ggagggttgt ttatttttgt cctact
//
LOCUS       DATIAL010000390         3926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_179793, whole genome shotgun sequence.
ACCESSION   DATIAL010000390 DATIAL010000000
VERSION     DATIAL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179793"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VLA60_12085"
     CDS             <1..828
                     /locus_tag="VLA60_12085"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HSF10147.1"
                     /translation="RTNPKSSTGRLDIFTRVVTDLHMGFDEIRSGYEGPLFLEIVPRS
                     FTIRVQAGLALNQLRLLSGKPMVSDSGLRAVHRKTPLLFHNGDSGKTDLALSAKELRV
                     NNGLFLSVDLQGSSDHSEAIVGYRAKKNSHVIDLSLVGHYVASDFWEPLKRNATANML
                     LEPEEFYILASKERIQVPPGYSSEMVAYEAACGELRTHYAGFFDPGFGYANRSRKGTQ
                     VVLEVRPHDVPFRIHDGQTFFKVVYEKMRDIPTQLYGSALGSSYYQQGLTLSKHFKW"
     gene            831..1262
                     /locus_tag="VLA60_12090"
     CDS             831..1262
                     /locus_tag="VLA60_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10148.1"
                     /translation="MSTETEQPQSTDPVAPKPVPPRPPVRDEDPEDQENLQMQVAMRM
                     VGSAMVFIGFLQVFLSASTGAEISIFPMIIYFSGMAMWAYSSVDNLTVRYTVMAVSLL
                     CAMAFMHFGEVLFWHKYLIYWGTIGVVVFFMFKNPKKPAGS"
     gene            1318..2016
                     /locus_tag="VLA60_12095"
     CDS             1318..2016
                     /locus_tag="VLA60_12095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSF10149.1"
                     /translation="MGIAVIIPVLNEQKALPALLPVLIPLEFEEIIVVDGGSWDGTVD
                     VARKMLESISDSRYRIISGPCGRASQMNAGAALANSEILVFLHADTQLPDNARQVIAD
                     AMDDPQCVGGRFDVRFPRDTGYAWMVSRLMNLRSRWSGIFTGDQTMFVRRSVFETLGG
                     FANIPLMEDIEFSRRLKRVGTIAALRAKVITSFRRWEQQGPLRTIVRMWTLRAWYRLG
                     WDPRRLQQYYDTVR"
     gene            2000..2773
                     /locus_tag="VLA60_12100"
     CDS             2000..2773
                     /locus_tag="VLA60_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HSF10150.1"
                     /translation="MTPSGNDPAVRRSRPSHQSQGGRRRSLPPAASAIIVFAKAPVAG
                     QVKTRLCPPLTSDEAASLHGSLVLDILERCQSLRGYDRILAGTPSPHHPFFRAMEARF
                     KIPVWDQIGDDLGTRMASVFKQALGSPYRSVVVIGTDIPGINGPLLTTALDSLHDHDV
                     VLGPTTDGGYYLIGLRAPVPELFENIPWSTEQVYALTEQKVKMLGLSLKILPKLRDLD
                     TVEDLHMCIRDSKDRQNQMFSSRTKNVLQELAKRLTNRE"
     gene            2836..3570
                     /locus_tag="VLA60_12105"
     CDS             2836..3570
                     /locus_tag="VLA60_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF10151.1"
                     /translation="MIDGVALITGGARGIGRGIALDLADKGWRVALCYRTSRQEAEEV
                     KGGIEGRGGKAMAVQCDVSDPVAATQLVQHVEDAWGKIDALINGAGPYHRISLFEETV
                     EGWSDMFTNNLHPIFYLAKAVAPGMKARKSGRIISFSMANADKMEAQPHVTAHYIAKA
                     GVLILTRTLAKMLAPDGITVNAISPGFIDSGSAPPGELEGMAKKIPAGYIGQVEDTVQ
                     AVRYFLSDEARYVTGANLHVSGGWGV"
     gene            complement(3662..>3926)
                     /locus_tag="VLA60_12110"
     CDS             complement(3662..>3926)
                     /locus_tag="VLA60_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10152.1"
                     /translation="GVRISRGVTMHGFALNVNVDLQPFTLLAPCGIEGCTMVSMAKNL
                     GRTIDSHRVRTQIAHHFGKIFGITWLEENGPGYEQEAGREARY"
BASE COUNT          875 a          967 c         1096 g          988 t
ORIGIN      
        1 cgaactaatc caaaaagttc cacggggaga ttggatattt ttacccgcgt ggtgactgat
       61 ctccatatgg gctttgatga aatccgctcc gggtatgaag gcccgttatt tttggaaatt
      121 gtgcctaggt cgtttaccat acgtgtgcag gctggattgg ccctcaacca attacgatta
      181 ctgtctggca aacccatggt ctcggattca gggttgcgag ccgttcatcg caagaccccg
      241 ttgctgtttc acaatggaga ttccgggaaa acggatctgg ccttatcggc caaggaactc
      301 cgggtaaaca acggtctgtt tttaagtgtc gatttgcaag gatcgagtga ccattccgag
      361 gcgattgtgg ggtaccgggc aaaaaagaac agtcatgtga ttgatctcag tctggtgggc
      421 cattatgtgg cctccgattt ttgggaaccg ctaaaacgca atgccactgc taacatgctg
      481 ttggagccgg aagaattcta tatcttggcc tccaaggagc gaatccaggt tccacctggc
      541 tattcatcgg aaatggtagc gtatgaagct gcctgcgggg aattgcgtac ccattatgcg
      601 gggtttttcg atcctgggtt tggctatgcc aaccgcagcc ggaaaggcac ccaggtggtc
      661 ttggaggtcc gtcctcacga tgtgcctttc cgtatccatg acggacagac gttttttaag
      721 gtcgtttacg agaagatgcg ggatattccc acgcaattat atggatcggc gttagggtct
      781 tcctattacc agcaggggct cacactcagc aaacatttta agtggtaact atgagtacag
      841 aaaccgaaca acctcagtcg accgaccctg ttgcccccaa gccagtgcct ccccgtccgc
      901 cagtcaggga tgaggacccg gaggatcagg aaaacctgca gatgcaggtg gccatgcgta
      961 tggtggggtc cgccatggtc tttatcggat ttttgcaggt ttttctgtcc gccagtaccg
     1021 gtgccgaaat cagcatcttc cccatgatta tttatttttc agggatggcc atgtgggcct
     1081 attcctccgt ggacaacctc accgtccgtt atacggtgat ggcggtgtct ctgctttgcg
     1141 cgatggcatt tatgcatttt ggggaagtgt tattttggca taagtacctg atttactggg
     1201 gcacgatcgg ggtcgtcgtg ttttttatgt tcaaaaatcc caagaaaccc gccggctcgt
     1261 agcccaacgt tcttaattct tggtctttcc aatcgtgcgt caaccattag gtttcccatg
     1321 ggcatcgccg tgatcattcc agtcttgaat gaacagaagg ctttgccggc tttgttgccc
     1381 gtcttgatac cgctggagtt tgaggaaatt attgtggtag atggaggaag ctgggatggc
     1441 acggtggatg tggctagaaa gatgttagaa tcgatatccg actcccgcta tcggatcatc
     1501 tccggtccat gtgggcgggc ttcccaaatg aacgccgggg cagctttggc caactccgaa
     1561 attttggtat ttttgcatgc tgacacccaa ttgccagaca atgccaggca agtgatagca
     1621 gacgccatgg atgatccgca gtgtgtcggt ggacggtttg atgtacgatt tccacgggat
     1681 actggttatg catggatggt gagccggctc atgaatctgc gctcacgctg gtcggggatt
     1741 ttcactggcg atcaaaccat gtttgtgcga cgttccgtgt ttgaaacgtt ggggggtttt
     1801 gcgaatattc ctcttatgga agatattgaa tttagccgcc ggttgaagcg agttggcacg
     1861 atcgcggctc tgcgagcgaa ggtcatcacc tcgtttcgac ggtgggaaca gcagggccct
     1921 ctccgcacaa tcgtgcgcat gtggacgctt cgcgcgtggt atcggctagg atgggatccg
     1981 cgtcgtcttc agcagtatta tgacaccgtc cggtaacgac ccagctgtac gtcgatcccg
     2041 tccatcacac cagagccagg gtgggagacg tcgatctctc ccgcctgcgg cgtcggcgat
     2101 tattgtcttt gcgaaggcac cggttgccgg tcaagtcaaa acacgcctgt gtcctcctct
     2161 cacgtctgat gaggcagcca gtctccacgg aagtcttgtc ttagatatcc tggagcgatg
     2221 ccaatctctc aggggctatg atcgcatcct ggccggaacg ccctctcccc atcatccctt
     2281 ctttcgggcc atggaggctc gattcaagat tccggtctgg gatcaaatag gggacgatct
     2341 gggaactcgc atggcatcag tattcaaaca agcgctcggt tccccatatc gttcggttgt
     2401 ggtcattgga acggacattc ccggcataaa tggtccactc ctcacaacgg cactagacag
     2461 tcttcacgat catgatgtcg tgttaggtcc caccacggac ggagggtatt atttaatagg
     2521 gttgcgcgct cccgtgccgg aactgtttga gaacataccg tggtccacgg agcaggtgta
     2581 tgccctcaca gaacagaagg taaaaatgct gggcctctcg ttaaaaatcc taccgaaact
     2641 tcgtgatctt gatacggtgg aggatcttca catgtgtatc cgggattcga aagatcggca
     2701 gaatcagatg ttttcttctc gaaccaaaaa tgtcttgcag gagttggcca agcgattgac
     2761 caaccgcgaa taagggaaca ataattcttt tggatggcga cggtgtccgc acgagtacca
     2821 caggagagat gagaaatgat tgacggtgtg gcgttgatca ctggcggagc cagaggaatt
     2881 ggacggggta tcgcgctgga cttggcggac aagggttgga gggtggctct gtgctatcgt
     2941 accagtcggc aggaagctga ggaagtcaaa ggtggcattg agggacgggg tggaaaggcc
     3001 atggccgttc aatgtgatgt ctccgatcct gttgcggcaa cacagcttgt tcagcatgtg
     3061 gaagatgcct ggggcaagat tgatgcgctg atcaacgggg ccggccctta ccatcgcatc
     3121 tcactgtttg aggaaactgt tgagggatgg tcggacatgt tcaccaataa tctccacccg
     3181 atcttttacc tcgcgaaggc cgtggcgccg gggatgaaag ccagaaaatc tggccgcatt
     3241 atttcgttta gcatggccaa cgcagacaaa atggaagccc aaccgcatgt gaccgcccat
     3301 tatattgcta aagccggcgt gctcattctg acccggaccc tggcgaaaat gttggctccg
     3361 gatgggatca ccgtgaacgc gatttcaccg ggatttatcg attcagggag cgcgccaccg
     3421 ggagagcttg aaggcatggc taagaaaatt ccggcaggat atattggtca ggttgaggat
     3481 accgtacaag ccgttcgtta tttcctctct gacgaagctc gatacgtgac gggagccaat
     3541 ctgcacgtga gcggcggctg gggagtctga agtctcgaag ctggcatcaa tgagcagaat
     3601 acaggcattc tcaaaatttt taccctcctt gtgccatgcc ggactaacgg cagagcgtct
     3661 ttcaatatct tgcctctcgt cccgcttctt gctcgtaccc gggtccattc tcttctaacc
     3721 aggtgattcc aaaaatcttc ccaaagtggt gggcgatttg tgtccgcacc ctgtgtgaat
     3781 ctattgttct cccaaggttc tttgccatgg aaaccatagt acagccttca atgccacatg
     3841 gcgctaagag ggtgaacggt tgaagatcga cgttcacgtt taaggcaaag ccgtgcatgg
     3901 tcaccccacg ggaaattcgt acgccg
//
LOCUS       DATIAL010000391         9783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1039194, whole genome shotgun sequence.
ACCESSION   DATIAL010000391 DATIAL010000000
VERSION     DATIAL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039194"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VLA60_12115"
     CDS             <1..636
                     /locus_tag="VLA60_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10153.1"
                     /translation="NVIILHFHERIARALHKIQMAMEVGRPTTFIIEQVAEMSEMLQV
                     FLDNLKAGVLLGNKFEVALKTLIEDLSREASFPILVYVDPSCEDHLSREQGAQLLHIV
                     REAVRNSVQYSHGKRGQVSAKTTPTDTVIEVSDNGKGFEVDLVGAQGHGLGNMVNRAK
                     KIGARLKIHSQPSKGTSVVIEVPLNEASSDRSHSASIVDASLSASKNVHVG"
     gene            complement(717..792)
                     /locus_tag="VLA60_12120"
     tRNA            complement(717..792)
                     /locus_tag="VLA60_12120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(757..759),aa:Met,seq:cat)
     gene            996..2249
                     /locus_tag="VLA60_12125"
     CDS             996..2249
                     /locus_tag="VLA60_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF10154.1"
                     /translation="MTSTAAQNIKQSVLVGRQAIFDRQKGVFGYELLYRDGQANSAQV
                     VDGDEATARVMVNTFLEMGIDQIAGTSQAFINLTANFFLSQNYEVLPKHNVVLEVLES
                     IEPTPIVIQSLVRARQLGYKIALDDFIVRDSHRALLEVADFVKVDILALTSEQLLEQI
                     DVLKQYPVQLLAEKVEDQEVYSLCYEKGFEYFQGYFFCKPQIMEGVKLSGNRMAIVLL
                     LAKLQDPDIQIKDLEELVENDLSLSLKLLRFVNSASVGLPRVVNSIGQAVGMVGTERM
                     RQWASLLVLAHTGGKPSELMRIALIRGRMCQGLSRLQGESTSQGFTVGLFSVLDAYFD
                     CEMKQLLADLPLASEILLALTEGQGCLGEILKCVMSYERGEWDQIENSCFEPHVVRQE
                     YFLSTEWANEVMKTTLAGNENSREG"
     gene            complement(2273..3439)
                     /locus_tag="VLA60_12130"
     CDS             complement(2273..3439)
                     /locus_tag="VLA60_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10155.1"
                     /translation="MSTTLRFEHIESAIQALPTQGRTMLQLLLLQYMNLSSEAIDYMV
                     SDQPDSRFLAGNQPKGNPLSLEAERNITSRANQYKDYYRQKRERPGMHIEFLTQALKN
                     IDKSIQIAERLLVSEFGLAQNALQDAKTQAPSILLRQELRKLQRAWDNQELSPKEYQI
                     QRLLLEYQALLRRRGIFRRRLKFAQNEFIASGNSPLKDHEIAHVWGIPLGSLVARKVK
                     ALQHFLTELQKYEEKLSTPNESLQPINLWQETLALLSQRPIERSMVEYDGLEKTEEAL
                     LDKLRAFVDGSMSEPEESKFWTSITKINDTEFSGTWKSHARSILAFQRLHALLNDMDF
                     SDEGLEESLRIKIYPQLPDDQLAPESDDKPIELSEKGLGVLNYFVGEPDDKRRG"
     gene            complement(3488..3703)
                     /locus_tag="VLA60_12135"
     CDS             complement(3488..3703)
                     /locus_tag="VLA60_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10156.1"
                     /translation="MEGEKDILFVRRDKDGAVTLYIDEDWAAERGVDPSQLVKIEIPR
                     ELYANGTVQQLREYAATCLESLDNGTP"
     gene            complement(4101..4808)
                     /locus_tag="VLA60_12140"
     CDS             complement(4101..4808)
                     /locus_tag="VLA60_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HSF10157.1"
                     /translation="MAGAEEAAKQQFVNFAFYKVDPLWRRLPKEERDLGKQEFIRAAE
                     EYSGKVIVVPYTTTGIRGDCDFMLWRISYDLEMFQEMSSKLLATGLGRYLTTPYSYLS
                     MTKRSIYVDHHVHEGQESRRLKISPGRAKYIFVYPFEKTREWYLLTKAARQGMMDEHI
                     AIGHKYPSVKLNTTYSFGLDDQEFVVAFETDSPQDFLDLVMELRSTEGSRYVKRDIPI
                     FTCIMKSLKEAVETLGG"
     gene            4997..5656
                     /locus_tag="VLA60_12145"
     CDS             4997..5656
                     /locus_tag="VLA60_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10158.1"
                     /translation="MNQILLFQLFTQRVGSMLGLVRCAGFWFLLEAGLVILVSSPVWG
                     IVLHPSESQILEAIENGQEGARSRTPPNMLYWHFGISSEDAPHAHGFLMTKLNGIAVM
                     SSHFALRGEGLASQDIQRILDEESLQVVVLIFGDSPSFAKDSYLLLKQGDRLIKPDRI
                     RFDARATLLSPSQGPPMYRAKIVASFNYDVFDALAQTTVKVFPGTGGEVTFDLDFASI
                     P"
     gene            5666..6991
                     /locus_tag="VLA60_12150"
     CDS             5666..6991
                     /locus_tag="VLA60_12150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HSF10159.1"
                     /translation="MGKRLFDAIGLGLLNMKPLHADIIAVGSELLLGGRLDSNSVFVA
                     HLLAECGIEVRKKISVGDQKGDIQEALRGSLKRAHVVVLTGGLGSTLDDCTREAVAAT
                     LQCPLLKRKKAYDNLQACYRRFGRPVTPLLARQAFLPSRATMLANTVGTAPGFLIRSG
                     QSVIIALPGVPREAKVMMVSQVQPILRKLFNSNRHYWQHTFQTFGLPETDVQRQLNSV
                     SKDYGSIQFGILASPRGVMVTVSCWVFGRRPSSVKEVSPFFPEWDHLILQIRECLGPW
                     LFAEGERTMEEVVGDALRGRSWTLALAESCTGGLVAHRLTGVPGSSRYVDRGVVSYSN
                     EAKRELVGVPASTLRRYGAVSSQVAMAMAKGIRMRSRVDVGVGVTGIAGPGGGTLKKP
                     VGLVYGAIDGPQGPQCQCWRFHGDRAEITLKTSQAVLDLIRRYANEAAF"
     gene            6975..7589
                     /gene="thpR"
                     /locus_tag="VLA60_12155"
     CDS             6975..7589
                     /gene="thpR"
                     /locus_tag="VLA60_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HSF10160.1"
                     /translation="MKLHSNQKSLRVFLAVELSLDLRRKVAELQRQLRESLPMVNWVR
                     PESIHLTLKFLGYVDASMVEPVLTAIEPIRTSQPPLTLELQGLGVFPHLRRPRILWIG
                     CTGDIPALLNLVSRIEGALEPLGFPPEDKPYFPHLTLARIKHDQSQVGGVLIHSGVLE
                     QPQNLGILHVERITLFRSEVSQSGAEYTALRTVPFNEPGSHISI"
     gene            7673..8773
                     /gene="recA"
                     /locus_tag="VLA60_12160"
     CDS             7673..8773
                     /gene="recA"
                     /locus_tag="VLA60_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HSF10161.1"
                     /translation="MAERVAPQKETQKDGKKRALDLALTQIEKQFGKGAIMKLGTEDV
                     PRDIPAISTGSLGLDMALGIGGLPRGRIIEVFGPESSGKTTLCLHAIAEAQKAGGAAA
                     FIDAEHALDISYAKKLGVHTDDLLVAQPDTGEQALEIAETLVRSGALDIIVVDSVAAL
                     VPRAEIEGEMGDSHMGLQARLMSQALRKLTGAISKSQASVVFINQIRMKIGVMFGNPE
                     TTTGGNALKFYSSVRLDIRRIESIKEGQEVMGSRVRVKVVKNKMAPPFKQAEFDVMFA
                     EGISKVGELVDLGVERRIVDKAGAWYSYKEERLGQGREAVKTFLKSNPDIAQDIDTQL
                     RNSYGLSGEPPKQEESSPPVSSTSKKSAGDRD"
     gene            8801..>9783
                     /locus_tag="VLA60_12165"
     CDS             8801..>9783
                     /locus_tag="VLA60_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HSF10162.1"
                     /translation="MKIIDMIATSLELRQAFVRYFSSCGHTVVPSGPLIPQADPTLLF
                     TNAGMNQFKGVFLGEESRPYSRAVSIQKCLRAGGKHNDLENVGFTRRHHTFFEMLGNF
                     SFGDYFKEEAIAFGWEFLTQIAGLPADRLWVTVFREDQEAYDLWHTRMGISESRLVRL
                     GEKDNFWQMGETGPCGPCSEILIDQGEAFGCGRSTCAVGCDCDRYLEIWNLVFMQFDR
                     DPVGTLNPLPRPSIDTGMGLERLAAVTQGVASNYDSDVFSPILERIGKSTGQVYGASP
                     TADRSMRVIADHLRAMTFLITEGVLPSNEGRGYVLRRIMRRASRHGRVLGV"
BASE COUNT         2391 a         2082 c         2619 g         2691 t
ORIGIN      
        1 aatgttatta ttctgcattt tcatgaacga atagcccgcg ccttgcataa aattcaaatg
       61 gctatggaag tagggcgtcc caccaccttc attattgagc aggtggcgga aatgtcagaa
      121 atgctccagg tgtttttgga caatctcaag gcaggagtcc tgctcggaaa caaatttgag
      181 gtggcactta agacccttat tgaggatttg agtcgagaag cctcattccc gatattggtc
      241 tatgttgatc cttcttgtga ggatcatctg tcacgggagc agggtgccca actgttacat
      301 attgtacgag aagccgttcg gaatagcgta caatattcgc atggcaaaag agggcaagtg
      361 tctgcaaaga ccacgcctac agacacggtt attgaggtct cggataatgg aaagggcttt
      421 gaagttgatc ttgtcggggc tcagggacat ggattgggaa atatggtcaa tcgggcaaag
      481 aagatagggg ctcgtctgaa aatccacagt caaccgtcta agggaacttc ggtagtcatt
      541 gaagtgccat tgaatgaagc gtcgtctgat agaagtcatt ccgcttcaat agtagatgcc
      601 tcattgagtg caagtaaaaa cgttcatgtc gggtgacgtt tgtgtccgat gcctcagaag
      661 ggggagaacc ttctggcgtc ttctgggatc cgtgaggcaa aggggaagaa ggaacttggt
      721 ggcggtggag ggatttgaac ccccggcccg cggcttatga gtccgctgct ctaccacctg
      781 agctacaccg ccgtatttca cccagaacga aaagatctat accatagctg gagaacttgg
      841 gacaattctt tttctgaaaa tgatccagtt ttaggtgaat ttcctagctc tagtgccctg
      901 gattccttta gagccgcctt tacttgatcc ttgtcggacc tagggatcgt ggcgatcctt
      961 ttaaaatttt tatttattta ccttggaagg tttttatgac ttcaacggcc gctcaaaaca
     1021 tcaagcaatc cgttctggtc gggcgacaag ctatttttga tcgtcaaaaa ggagtttttg
     1081 ggtatgaact cctgtatcgg gatggtcaag ccaatagtgc gcaagtagtt gacggggatg
     1141 aggccaccgc cagggtcatg gtgaatacgt ttttagagat gggaattgac caaatagctg
     1201 gaacgagtca ggcgttcatc aacctgacag cgaatttctt tttaagccaa aattatgaag
     1261 tcctgccaaa gcacaatgtg gttcttgagg tgttggagtc cattgagcct acgcccatcg
     1321 tcatccaatc gcttgtcagg gctaggcaac ttggctacaa aattgcgtta gatgacttta
     1381 tcgttcggga ttctcatcga gcattactag aggttgctga ctttgtaaaa gtggatatat
     1441 tggcattaac gagtgaacaa cttcttgagc aaattgatgt gttgaagcaa tacccggttc
     1501 aattgcttgc agaaaaagtc gaagatcaag aagtgtattc gctgtgttat gaaaaaggat
     1561 ttgaatattt tcaagggtac tttttctgta aaccgcaaat catggaggga gtcaagcttt
     1621 ccggtaatcg catggcgatc gtgcttttat tagccaaatt gcaagatcct gatattcaaa
     1681 taaaagatct tgaggaatta gtcgaaaatg acctgtcact gagcctgaag cttcttcgtt
     1741 ttgtgaactc tgcgtctgtg ggtcttcctc gagtggttaa ttcaattggg caggccgtgg
     1801 ggatggtagg gaccgaacgt atgcggcaat gggcctcctt actggtgctg gctcatactg
     1861 gtggaaagcc aagcgagctg atgcgcattg ccctcatccg gggacgaatg tgtcaaggtt
     1921 tgagccggtt gcaaggagag tctaccagcc aaggctttac cgttggcctc ttttctgtgt
     1981 tggatgccta ctttgattgt gagatgaagc aacttctcgc agaccttcct cttgcttcgg
     2041 aaattctgct tgctctgacc gaagggcagg ggtgtttagg ggaaatttta aaatgcgtga
     2101 tgtcttacga acgaggggag tgggatcaaa ttgagaacag ttgttttgaa ccccatgtgg
     2161 tgcgacaaga gtattttctc agtactgaat gggccaacga ggtcatgaag acgactcttg
     2221 ccgggaatga aaatagcaga gaaggatgag gtggaaggtt ggaaaaccag gattatcccc
     2281 ttcgtttgtc gtctggttct cctacgaaat aattcagcac acctaaaccc ttctcgctaa
     2341 gctcaatagg cttatcatcg gattcagggg ccagttggtc gtcagggagt tgggggtaaa
     2401 ttttgatccg taggctttcc tccaaccctt catctgaaaa atccatatca ttaagcagag
     2461 cgtgtaatcg ttggaaagct aaaatagaac gagcatggga tttccatgtt cccgagaatt
     2521 ccgtgtcatt tattttggta atgcttgtcc aaaactttga ttcttcaggc tctgacatgc
     2581 ttccgtcgac gaatgctcgt aatttatcaa gtaacgcctc ctctgttttt tccaaaccat
     2641 cgtattcaac cattgacctt tcgatggggc gttgggataa gagggcaagt gtttcttgcc
     2701 agaggttaat tggttgtaac gactcattcg gtgtggagag tttttcctcg tatttttgta
     2761 attcagtcag aaagtgttga agggccttaa ccttcctggc aactaggctc cctaaaggaa
     2821 ttccccagac atgagcaatc tcgtgatcct ttaacgggga atttccggaa gcaatgaatt
     2881 cattttgagc gaacttcaat cgacgtctga agatcccacg tcgtcgcaat aaggcctgat
     2941 attccaggag cagtcgctgt atttgatact ccttcgggga gagttcctga ttatcccatg
     3001 cacgctgtaa tttccggagt tcctgtcgaa gtagaatcga gggtgcttgg gttttagcat
     3061 cctgcaaggc attctgggcc aatccaaatt ctgatacgag taaccgttct gcgatctgaa
     3121 tggatttatc aatgtttttc aatgcttgag tcaggaattc aatgtgcatt cctgggcgtt
     3181 ctcgtttctg tcggtagtaa tctttgtact ggtttgcccg ggatgtgata tttctttcgg
     3241 cttcaagaga gagcgggttt ccttttggct gattgcctgc cagaaagcgg gaatcgggtt
     3301 gatcgctgac catatagtcg atggcttcag aactcagatt catgtactgc aacaaaagaa
     3361 gctgcagcat ggtgcggcct tgagtgggaa gcgcctgaat ggctgattca atgtgttcaa
     3421 aacgcagtgt tgtagacata attatttatg agctcaagga caagattttg tatttcaacc
     3481 tttagtttta aggagtgcca ttatcaagag attcgaggca tgtggctgcg tattctctta
     3541 gttgctgtac tgttccattg gcatagagtt ctcggggaat ttcaattttg actaattgcg
     3601 atggatcgac cccccgttct gcggcccaat cttcgtcaat atataaggtt accgcgccat
     3661 ctttgtcccg tcgcacaaaa agaatatctt tttccccttc catctctgac actcccgaat
     3721 gaaaattaca ctttgttttt cttgataaca cacctctttg aggcctgtca aaatggtcga
     3781 gtgccttggt gtgggttaac cagatgttct gagactcaag ggattttatt agaaatttgt
     3841 tgctgcaggt aggagaatcg ctaaggcatg aggtgcggct gttgtaactg atgaaatcat
     3901 ccgtacgcac tgtcgggcag gagcaccatc aagtgtcagg cctatctagg attgaggaaa
     3961 gttttaattt atcgggaaaa tttgaattat aagcacatac agataccctt caatggaagg
     4021 gtatctgtta aagagctcat tttggaatga agtctggagg ctgctgctct aaagtgcgtt
     4081 acgagaatga ttgagagaga ttaccctccg agagtttcaa cagcttcttt gagacttttc
     4141 ataatacagg taaaaattgg aatatcacgt ttgacatacc ggctgccttc ggtagagcgt
     4201 aattccatga caaggtctag aaagtcctgt ggggaatcgg tttcaaaggc gaccacaaat
     4261 tcctggtcat ccaatccaaa tgaataagtg gtattgagtt tgactgaggg atacttatgg
     4321 ccaatagcga tatgttcatc catcatgcct tggcgggcgg ctttggtgag caggtaccac
     4381 tctctggttt tttcaaatgg ataaacaaat atatatttcg cacggccagg ggaaattttc
     4441 aatcttcgac tttcttggcc ctcatgcacg tgatgatcga cgtatatgga tcgtttcgtc
     4501 atggaaagat aagagtacgg cgtggtaagg taccgtccca agcctgtagc caagagcttt
     4561 gaactcattt cttgaaacat ttcgagatca tagctaattc gccacaacat aaagtcgcaa
     4621 tcacctcgta tgcctgttgt ggtgtaaggc acgacaatga ccttgccgct atattcttcc
     4681 gctgcgcgga taaattcttg cttgccgagg tctcgctctt ctttgggaag acgtctccac
     4741 aaaggatcga ctttatagaa agcaaaattg acaaattgtt gtttggcagc ctcttcggca
     4801 ccagccataa tcatacctcc cctggaaaga aaacccgtgt ttttttaatc acttaaaaaa
     4861 ataggtctta acatcgctac tgcaatattg tcaatgagca gctggcgtga tggctgttaa
     4921 gatccactcg gaataggagg agtagggaat aggaccttaa tcttgaggag gggagaaact
     4981 ttttaggtca tagccaatga atcaaatctt gttatttcaa ctatttaccc aacgcgtagg
     5041 gtctatgctg gggttggtaa gatgtgccgg tttttggttt cttttggaag cggggctggt
     5101 catcttagtt tcttctccgg tgtggggtat tgttctgcat ccctcggaat ctcaaattct
     5161 tgaagccatc gaaaatggtc aggagggagc ccgaagccga actccaccca atatgttgta
     5221 ttggcatttc ggtatatctt ctgaagatgc tcctcatgcc catggatttc taatgacgaa
     5281 attaaatgga attgcggtga tgtccagtca ttttgctctt cgaggagaag gcctcgcatc
     5341 ccaggatatt caacgaatcc tggatgaaga atcccttcaa gtcgtggtgt tgatctttgg
     5401 ggattcacca agctttgcca aagacagcta tcttttgctt aaacagggag atcggttgat
     5461 caagcctgat cgcattcggt ttgatgccag agcgacgctg ctcagcccaa gccaaggacc
     5521 tcccatgtat cgtgcaaaga ttgtggcatc ctttaactac gacgtttttg atgccttggc
     5581 tcaaaccacc gtcaaagttt tcccaggaac aggaggcgag gtgacttttg atctagattt
     5641 tgcttccatt ccataagggg cgtggatggg taaacgactg tttgatgcaa ttgggctggg
     5701 tctcttgaac atgaagcctc ttcatgctga catcattgcc gtgggttcag aattgcttct
     5761 gggcggacgc ctcgatagta attcagtatt tgtcgcacat cttctcgctg agtgtggcat
     5821 tgaggttcgg aaaaaaatat ccgttggtga ccaaaaaggg gatattcaag aagcccttcg
     5881 tggctcgctg aagcgcgctc atgtggttgt cttgacgggt ggactcggtt caacgctcga
     5941 tgattgtacc cgtgaggctg ttgccgcgac gcttcaatgt ccactgctca aacgaaaaaa
     6001 agcttatgac aatcttcagg cttgctatcg acgctttgga cgaccggtca caccgctatt
     6061 ggccaggcag gcttttcttc cttcccgagc cacgatgttg gcgaataccg tcggcacggc
     6121 gcccggtttt ttgattcgta gcgggcaatc agtcattatc gcattaccag gtgttcctcg
     6181 tgaggccaaa gtcatgatgg tttctcaggt tcagccgata ctccggaagt tatttaacag
     6241 caacaggcac tattggcagc ataccttcca aacatttgga ttgcccgaaa ccgatgttca
     6301 aagacaattg aattctgttt cgaaggatta tggaagcatt cagttcggta ttttggcttc
     6361 gcccagggga gtgatggtga cagtgagttg ttgggtgttc ggccgacgac cttcttctgt
     6421 gaaggaggtg tccccgttct tcccggaatg ggatcacctg atccttcaga tccgtgaatg
     6481 tttaggtccc tggctctttg cggaaggtga acgtaccatg gaagaagtcg tcggggatgc
     6541 cttacgaggt cgttcctgga cgctggcact tgccgaatcc tgtaccggag gattagtggc
     6601 tcatcgcttg acgggtgtgc ccggcagttc gcgttatgtg gaccggggag tcgtgtctta
     6661 tagtaatgaa gcgaaacgag aactggtggg agttcctgcc tcgacgcttc gtcggtatgg
     6721 ggccgtgagc tcacaggtag ccatggctat ggccaaagga attcgcatgc ggagtcgagt
     6781 cgatgtggga gttggagtca caggaattgc gggtcctgga ggagggactc tgaagaagcc
     6841 agtggggctg gtgtatggag cgattgatgg tccccaaggg cctcagtgtc aatgttggag
     6901 atttcatggg gatcgagcgg aaattacttt aaagacctct caggctgttt tggatttgat
     6961 ccgacgatac gccaatgaag ctgcattcta atcagaagag tctgcgagtc tttttggccg
     7021 ttgaactttc cttggatttg cgtcggaaag tggccgaact tcaacgtcag ctgcgagaaa
     7081 gcttaccaat ggtcaattgg gttcgccctg agtcgataca cctcacactg aaatttcttg
     7141 gatatgtgga cgcttccatg gtggaaccgg ttttgactgc cattgagcct atccggacaa
     7201 gtcagcctcc tctaacctta gagcttcagg ggctgggagt ttttccgcat cttcgacgtc
     7261 cacggatact gtggatcgga tgtaccgggg acattcctgc cttgctcaat cttgtttcac
     7321 gaatcgaagg tgcgttggaa ccgttgggct ttcctcccga ggacaagccc tatttccctc
     7381 atttgaccct ggccaggatc aaacatgacc aatctcaggt tggtggcgtc ctgattcatt
     7441 ccggcgtact ggagcaacct cagaaccttg ggatactcca tgttgaacgg atcacacttt
     7501 ttcgcagtga ggtgagccaa tccggtgcgg aatatacggc tttgaggacg gtgccattca
     7561 atgagcctgg atcacatatt tcaatctaag ttaggcattt acctgttcaa gagtgtgggg
     7621 taagatagcc gccactttgc atgctcggaa atattgaaca aaaggatacc caatggcaga
     7681 acgcgtagct cctcaaaaag agacccaaaa agacggaaaa aaacgagcgc tggatttggc
     7741 tctgacccaa attgaaaagc aatttggcaa aggggccatt atgaagctag ggacggaaga
     7801 cgttcctcgt gatattccgg ccatttcgac ggggtctctc gggcttgata tggccttggg
     7861 gatcggagga ttgccgcgag gtcgaatcat tgaggtgttc ggaccggaat cgtctggaaa
     7921 aaccacgttg tgcctgcatg ccattgccga agcgcagaaa gccggcgggg cggcagcctt
     7981 tatcgatgcc gagcatgcgt tggatatctc ctatgctaaa aagcttgggg tccacaccga
     8041 tgatctgctg gtggcccagc cggatactgg cgaacaggct ctggaaattg ccgaaacgct
     8101 ggttcgaagc ggggcgttgg atatcattgt cgttgattcg gttgcagccc tggttcctcg
     8161 tgccgaaatc gaaggggaaa tgggagattc tcatatgggg cttcaggcta gattaatgtc
     8221 ccaggccttg cgaaaattaa ccggggccat ttctaagtct caagcttcgg tggtatttat
     8281 caatcagatt cgcatgaaaa ttggggtgat gtttggaaat cccgagacca cgaccggcgg
     8341 caacgcgttg aaattttatt cttcagtgcg gttggatatt cgccgaattg agtcgattaa
     8401 agagggccag gaagtgatgg ggagtcgggt acgggtgaaa gtggtcaaaa ataaaatggc
     8461 gccacccttt aaacaagccg aatttgatgt catgttcgcc gaaggtattt ccaaagtcgg
     8521 ggaattagtg gatttaggcg ttgagcgtcg aatcgtagat aaagccggag cgtggtattc
     8581 gtataaggag gaacggctcg gtcagggccg ggaggcggtt aaaacctttc tgaaaagtaa
     8641 tccggatatt gcccaggaca tcgacacgca attacgaaac agctatgggc tctcggggga
     8701 gcctccaaaa caagaggaat catccccgcc tgtcagttca acctcaaaaa aatcagcagg
     8761 agatcgtgac tgatctggcc gggaggctaa atcatcttga gtgaaaatca ttgatatgat
     8821 tgcaacatca cttgaacttc ggcaggcctt tgtaaggtac ttctcgagct gtgggcatac
     8881 ggtggtgccc agcgggccac tcattccgca agcagatcct acgctcctgt ttaccaacgc
     8941 gggtatgaat caatttaaag gtgtgttctt gggtgaggaa tcccgaccct atagccgggc
     9001 cgtctccatt caaaaatgtc tgcgggcggg gggaaagcat aatgatttgg agaatgtggg
     9061 ttttacccgc cgccatcata cgttttttga aatgctgggc aatttttcat ttggagatta
     9121 ttttaaagag gaagcgattg cgtttgggtg ggaatttctc actcagattg ccggactgcc
     9181 ggctgatcga ttgtgggtga cggtgtttcg tgaggatcag gaagcctatg atctatggca
     9241 tacacgaatg ggtatttcag agagtcgcct tgttcgtctg ggtgagaagg acaatttttg
     9301 gcaaatggga gagacgggtc cctgtggtcc ttgcagcgaa atcctcattg atcagggtga
     9361 ggcattcgga tgtggccggt cgacctgtgc cgttggctgt gactgcgatc gatacctcga
     9421 gatttggaat ttggtgttta tgcaatttga tcgggatcct gttggcacgc tcaaccctct
     9481 acccaggccc agtattgata ccggaatggg gttagagcgg ttggcagcgg tgacccaggg
     9541 agtggcgtca aattatgata gcgatgtgtt cagccctatt ttggaacgaa tagggaagag
     9601 caccggacag gtgtatggtg cctccccaac ggctgaccgt tcgatgcggg tgattgcgga
     9661 ccacctgcgg gcaatgacct ttttaataac tgaaggggtg ttgccatcga atgaggggcg
     9721 tggctatgtc ctgcgacgga ttatgcggcg agcctcacgg catggtcgag tattgggggt
     9781 gga
//
LOCUS       DATIAL010000392        12793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_610088, whole genome shotgun sequence.
ACCESSION   DATIAL010000392 DATIAL010000000
VERSION     DATIAL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610088"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /locus_tag="VLA60_12170"
     CDS             <1..1765
                     /locus_tag="VLA60_12170"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10163.1"
                     /translation="ADLIPPSWQYPEVTAARIRFGHLEITTPNFVVSPWTQREEFICR
                     DGTTGEIEVVYLEERPTEVEGPFLQEERNLLHSLGEMVRLQLDRKRTQAALREAHDVL
                     EQRVLDRTSDLTMANRLLSREVAQRKEAEEKLEDLIAEIQRNHDDLIAILNQLHIGTA
                     LTDRDGKVTFLSQAAQQLCGKANVPHSGEPWQALFSDPAYHAQIEDMVNRAPEERQEV
                     LVRMSAVGGECTRWINVDVRDDPRDPERKMFFLYDASEVQTLRQLLGETGKYQDLIGA
                     SHSMQLVFQQIQDFSRVDSTILIEGETGTGKELVARAIHQASHRNEFPFIAVNCAGLT
                     EALVASQLFGHKRGAFTGAVSDQQGLIEAAEGGTLFLDEIGDIPLTVQTSLLRVLQEW
                     EITRLGESRLRKVNVRVLAATHHNLANDVSAGTFRADLLYRIRVGRVLLPALRERRED
                     IPLLISHFLNQYRATTGRPVEAVSNEALRLLMAYAWPGNVRELKHAIEFAVIRCPQTI
                     IRPQDLPPEVLNQDERLSVAPSEDITSSHDDDKEKIFAALKQTGGNRSAAAKLLGIAR
                     LTLYRRMSRLGIPARRKTSSL"
     gene            2364..2735
                     /locus_tag="VLA60_12175"
     CDS             2364..2735
                     /locus_tag="VLA60_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10164.1"
                     /translation="MNHKIMMVFFLVGILLGIPNFVLAGEITKEQISEWKLETQGWSI
                     QDHIAAAYSEKEEVQSLEDRIQQLDKRISMLEKKPYFDPKGFTRSSLKLLSSTLEEQE
                     KLLNEKIAWHFRQADQAKLTE"
     gene            3290..3661
                     /locus_tag="VLA60_12180"
     CDS             3290..3661
                     /locus_tag="VLA60_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10165.1"
                     /translation="MNNKFFISAFLVGMLLGLPQFVLASDNGQEQSSAWRMETQGWSI
                     QDHMTAAKASEGEVQSLESRVQDIDKRIAHFESKPHFDPKGFHRNALKHISSTLKGNT
                     NILNEKIAWHIRQADQAKLTE"
     gene            4382..5929
                     /locus_tag="VLA60_12185"
     CDS             4382..5929
                     /locus_tag="VLA60_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10166.1"
                     /translation="MIVDMPHSTDQLARNFYQTLLEVTNVLNSQRNTEDLWKAITGHI
                     KKVISWERAGITLYHSDIDAFRFYAVETSMPVVKLKCDAIIPREGSAMGWVYNHRRLH
                     IRPHLQQNPVFLEDAFYHEEGLGRMINIPMIIGDNCIGSLNIGSVESGDPDPENVLFL
                     HQVATQIAFAIDHVRAYEEIHHLKEQLTRENAYLLEEIKVTYDFGAMVGNSQKFKNVL
                     DLAQAVAPTTTSVLIMGETGTGKELLARLLHELSPRKVKPFVRVNCAALPSGLVESEL
                     FGHEKGAFTGAETGRPGKFELANGGTLFLDEIGEMPIEAQAKLLRVLQDGIVERVGSI
                     EGKAVDVRIIAATNSDLSKAIAEGRFRSDLYYRLHVFPIVVPPLRERLHDIPLLVRHF
                     MEKNRLQFRRNCQEIDDRSMEMLVQYPWPGNVRELKNVIERAMILCQSSVLHVEDSLL
                     QSPANDMDAMPPTQLKDVERTRILQALAACEWRIDGPLGAAKHLGLHPSTLRSRLKKF
                     GIKRYSE"
     gene            complement(6362..7102)
                     /locus_tag="VLA60_12190"
     CDS             complement(6362..7102)
                     /locus_tag="VLA60_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF10167.1"
                     /translation="MGKLTKPSSYHVALRKRQEIAKGTMAFYFDRPAGFTFTAGQFID
                     LSLPNLSPSDPEGHTRALTLASAPSEQRLMVATRLRDTAFKRMLAEMPLGTTIDLEGP
                     FGQFTLPFDDSRTIVLLAGGIGITPFRSMLVEAAHHKLSHHPILLYSNRHQEDAAFLD
                     ELQTLQQENTHFTCIGTMTSPNGGTETWKGETGRIDPVMLRKYVKETETALYYVVGPP
                     AMVDGLRKMLESTGIPRTNIRSEEFVGY"
     gene            7363..8898
                     /locus_tag="VLA60_12195"
     CDS             7363..8898
                     /locus_tag="VLA60_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10168.1"
                     /translation="MERIERNYRALLNVAIVLNSQRDTDSLWQAIIEQIEQVMPWARA
                     SVTLYDRESDGFRFYVVTTHLAKVVLQGDSVIPRVGSGMGWVYDHKSMHVRPDLRQEQ
                     VFLEDQWYVEEGLGRMINLPLLVRGKCMGVLNLGSVDPGNPEPDALEFLSQVAMQIAY
                     AIDHVQAYEEINLLRQQLARENIYLVEELKLTKHFGAMVGRSQVFQHVLELAREVAPT
                     PATVLITGETGTGKELLAQAIHDWSMRANKPFIRVNCAALPAGLVESELFGHEKGAFT
                     GADSRREGRFELAHGGTLLLDEIGEIPLETQAKLLRVIQDGMVDRVGGKQPIPVDIRL
                     MASTNADLPSAIEQGRFRADLFYRLHVFPITIPPLRARPEDIPELAQHFLEHYAMKLK
                     RPCETIEPDSLQRLIRYSWPGNIRELENVIERAVILSHQKVLRIDERLLILQNATHES
                     KAPAGLVDFERQHILQTLASSNWQIEGEGGAAEQLGLAPSTLRSRIKKLGLQRPSSST
                     PPK"
     gene            9150..9983
                     /locus_tag="VLA60_12200"
     CDS             9150..9983
                     /locus_tag="VLA60_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10169.1"
                     /translation="MTISRTQSRVISERRTLLPVSVFAASLLFLIGVLIPVTSKAAET
                     NDGHHELLSGERILLATVEETSSADVRVDTGDLQPRYLPLNVRKDKGLPELQVGDRIA
                     ITVNDQNLIVDVHLLGEIDHHQIIRGVLAQPLMTGHEQAVIRTEDGKEESYVVRPLAR
                     SKVASVPVGAHAIFLLDERHGIADATYESAEAAAEAQQLRQKRSPLKSSFKRVTGVIL
                     KTLDHNTISFQAHEGSVQLLEVSPLVLDKLPKLPKGHLVVLFLDADNTVTDVSAYDRE
                     H"
     gene            10035..10499
                     /locus_tag="VLA60_12205"
     CDS             10035..10499
                     /locus_tag="VLA60_12205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10170.1"
                     /translation="MLTQGFPVEGLNTVGQIVATNTVRFHAQQNGLDIAGALLSTHMA
                     GSPVVDDQGRYVGFINEFDAMRALAAGNDLGKLVAKDIMRKDQLAVISSTPIEEAAKR
                     MEEYHVFNLPVIDQYGAVAYSVSWHDLLRAWIGPGVDPGVAGLSLWATNRGE"
     gene            10589..11233
                     /locus_tag="VLA60_12210"
     CDS             10589..11233
                     /locus_tag="VLA60_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HSF10171.1"
                     /translation="MVSLQKLIQYQLSEGLTEYELAEDIGVPKFTIQRILRGRDPESL
                     DIWKKLARYFHVDMELLRFGHFAFVHPPMKDEDRSDGPQPYRKVPILSWTQVRHVVEG
                     HDSKFFLAAGQGMVETELTGARVFAMHVEDDSMEPLFHVKEIIFVNPDLRPQSGDYVV
                     VLDQMAEADHGCLRQLKRIQKQYILQPLNLQYKDVNLASHQKIVGRVVRLRMNL"
     gene            11414..12706
                     /locus_tag="VLA60_12215"
     CDS             11414..12706
                     /locus_tag="VLA60_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HSF10172.1"
                     /translation="MTTREPSLTVYDVLIALGQFQGLSANPKLISSPLLAALKDAGTA
                     HIYADTASREELMTVLTVDQETILKEIDGNTVNQPLLAKVIKQYLKQDNPAKWAQHLR
                     EKEPELSQGDLLPLFYSIVCGKIGTDMVRAFASGRSWEVSLQLHMGLVHNLENAKQIG
                     RSLRRMVPSAFVKVPFAPHAPMCFLIARDLEKEGIPVNFTSTFSARQVVAAALLSNVT
                     RTNIFMGRLDQGLKAELLGAHVSLEAQRILLRLRGNSGIKTQLIVASLRDWGSLLHTA
                     GCDVFTVPCKVLQEFLEQNEVKDSSIQSRLGTSYEDRLGIPDNIVAKLGADRIARLYT
                     VEPEFVDFLLEYRVTTEYQDLDDGDHLVRRFEQAGFGDLFYAPDHHEWTALRRGKVPD
                     LEDPITMRPSLDTLYSLLADADFEKYQEDMDREMTRYL"
BASE COUNT         3335 a         2843 c         3375 g         3240 t
ORIGIN      
        1 tgccgatctt attccgccat cctggcaata cccggaagtc acagccgccc ggattcgctt
       61 cggtcatctg gaaattacga cgcctaattt tgtggtatcc ccgtggactc agcgggagga
      121 gtttatttgc cgagatggaa cgacgggtga aattgaagtc gtatatctgg aggagcgccc
      181 gacggaagtt gagggcccct ttctgcagga agagagaaat ttattgcact cactaggaga
      241 gatggtccgg ctgcagttgg atcggaagcg gacgcaagcc gctcttcgtg aagcgcatga
      301 tgtgctggag cagcgggtgc tggacagaac atccgacctg acgatggcca atcggttact
      361 gtcgcgggaa gtggcacaac ggaaagaagc ggaagagaaa ttagaggact tgattgcgga
      421 gatccagcgg aatcacgatg atctcattgc tattttgaat caattacata ttgggactgc
      481 cctgaccgac agggatggaa aggtgacctt tctgagtcag gccgcacaac agttatgcgg
      541 aaaagcgaat gttcctcatt caggtgagcc ctggcaagcg ttgtttagcg atcctgccta
      601 tcatgctcaa atagaggaca tggtgaatcg tgctcctgaa gagcggcagg aggtcttagt
      661 gcgcatgtct gcggttggtg gagagtgcac ccgttggatc aatgtggatg tcagagatga
      721 tccacgagac ccggaacgga aaatgttttt cttgtacgat gcctcggagg tgcagaccct
      781 tcgtcaatta ctgggagaga ccggcaaata tcaggatctt attggcgcca gtcattccat
      841 gcaattggtt ttccagcaga ttcaggattt ttcccgtgtg gattccacca ttcttattga
      901 gggagagacc ggaacaggca aggaactggt cgctcgtgcc attcaccaag ccagccaccg
      961 caatgaattt ccttttattg cggtgaattg tgcggggtta accgaagcac tggtcgccag
     1021 ccaattattt gggcataaac gtggggcctt cacaggcgct gtctcagatc agcagggatt
     1081 gatcgaagcc gctgaaggag gaaccttgtt tctggatgaa atcggggata ttccgctcac
     1141 cgtccaaacc agtctgcttc gggttctgca agaatgggaa attacccgtc ttggggaatc
     1201 tcggctccgg aaagtgaatg ttcgtgtgct tgcggccacc catcataatc ttgccaatga
     1261 tgtcagtgcg ggaaccttcc gggctgacct gttgtatcgg attcgggtcg gaagagtgtt
     1321 attgcctgcg ctgcgggaac ggcgggaaga tatcccatta ttaatcagcc attttctgaa
     1381 ccaatatcgg gcgaccacag gaaggcctgt tgaggccgtg agtaatgaag cgctccggct
     1441 gttgatggcc tatgcatggc ctggaaacgt tcgcgaactc aagcatgcta ttgaattcgc
     1501 cgtcattcga tgtcctcaga cgattattcg acctcaagat ttaccgccag aggtgcttaa
     1561 tcaggatgaa aggctttcag tggcaccctc tgaagacata acttcaagcc atgatgatga
     1621 taaggagaag attttcgctg cattaaagca aacgggaggg aaccgttcag ctgccgcaaa
     1681 gctgcttggg atcgcacgcc tcacgttgta tcgtcgcatg tcacgcctcg gaattcctgc
     1741 tcgccgtaaa acgtcttccc tataaagtct tgtttgggag acagttgaat aactcatcct
     1801 ccaaccatag ggaatttgcc cctattaagc ccaggcgaat ctgggtaggg gagatttcag
     1861 aaaggtccgg ctttaaataa gccaaccccg gcaggctaaa gcaaggtttt ccacagtccc
     1921 ggccgcaatc acctggatgg ccggagctcc cggattggcg gtggagcatg gcctagaagt
     1981 ctcctgtcgg cgtgcttgcg cgatttcccc gcacttcttt ccttgttcgt ctattccttt
     2041 agaaccgttt caaatatccc gcatcttcct atcttccaca gagtaaggcg ttgttcagca
     2101 tccagataaa taagaaacgc atctaaaggc atgacggact gacgtgttac ataatgcatc
     2161 aaggcattac acttttgatg cacatggatg taacagttca gcaccttcat tttattgcac
     2221 aaacattaaa ccgttgaaat taaaagtaag tactgcctta aggctgttgg cctataaatt
     2281 ggataaacat aataaggaga tgaaagagtg ggcagtgaat caatggatgc tctttaaggc
     2341 agtcagtaag aagggaggaa gccatgaatc acaaaataat gatggtattt tttctcgtgg
     2401 ggatactcct aggcattcca aattttgttt tggctggtga aattacgaaa gaacagattt
     2461 ctgaatggaa actggaaacc cagggttggt ccattcaaga ccatatagct gctgcgtatt
     2521 ctgaaaaaga ggaagtccag tctttagagg accgcattca gcaattagat aaacgcataa
     2581 gcatgttgga gaaaaagcca tacttcgatc cgaagggttt taccagaagt tccttaaagc
     2641 ttctttccag cacattggaa gaacaagaga agctattgaa tgaaaagatt gcctggcatt
     2701 ttcggcaggc cgaccaggcc aagctgacgg agtaggagta atggaacctc cagaatttcc
     2761 taatcctcgc atgagagtgt tcccttaagt ttttcttcgc agaaacagga ccgtcagcct
     2821 gctcaaccat gcgggtccaa tgccttgaat tgaataagaa gcatgagtga gtcggcaaag
     2881 cagagccaag actcttcgca ttttttttat gttccccacg gacactgatc atgggctgat
     2941 gggttacttc aggctggagg ggtagcacct tctctagccg gttccatctt cacatttctc
     3001 aggcaaaacc ctggtcaaca aaacaaaaaa aaagatctcc gaatggatga ctgaaaagtg
     3061 taacattgtg taactaagtg aatcacattt gttacatttg tatgttacat gtatgacata
     3121 atttttaagt acataaaatg gtaagttatt gaatataaag gtaagtataa tttaattttt
     3181 gttggcatat aaattggata ttgcaatagg gtgtgcggaa acaacgttaa cattagcagt
     3241 gcaagttcga cgatgtttta aatctgataa atgctggaag gaggaagtta tgaataacaa
     3301 attttttatt tcagcttttc ttgtggggat gcttctagga cttccacagt ttgtcctggc
     3361 tagtgataac ggtcaagagc agagttcagc ctggagaatg gagacccagg gttggtcgat
     3421 tcaagatcat atgactgcgg caaaagcttc agaaggggaa gtccagtctt tggaaagtcg
     3481 tgttcaggat atagataagc gcatagccca ttttgagagc aagccacact tcgacccgaa
     3541 ggggtttcat agaaatgcct tgaagcatat ctcttctacc ttgaaaggga atacgaatat
     3601 tctgaatgag aagatcgcgt ggcatataag gcaagctgac caagctaagc tgacggagta
     3661 gttgatccca gtatgatgta attgcaaatg ccaataaatg tctatccctg ttccatgtaa
     3721 tggggaggtt cagcagcctc cccattcccg gaacagaata aaatctccac cctttattat
     3781 tcttgtgcct gacgtatacg tcaggctttt ttttttctaa ggcttttgac ctccatggaa
     3841 ttgcatacct ttcacgaact gatgcaaaaa atataaggca aatcccttct tccacacatg
     3901 aagaagtggc ctcactggtg acacttatcc aaagcaatgc atctatgagg tgggataaaa
     3961 gaccagaggt gtggatatag ctgtggcgaa tgggcgtgcg attgagcacg ggcatctacg
     4021 tgcgttttcg ctcaattgcg aaaattaaaa atgaagtttt tttaataatg tttacttaca
     4081 taaaccaatt gattgcgtga catggacgac acaggtggga gaggtggtgg ttggacttat
     4141 taaactattg aaaaataaaa catattttta tgaatattcg ctcagttgac atgacaactt
     4201 tgaaaaggga aatagatatt tgtacaccac aacaaaagcg gttaatggct cagtaattgc
     4261 tgcttcttta tagaaattta atcgtaatat ttttacccat gaatacctgt ttcttgttca
     4321 aaaattacgg ttagggttaa aatccttcct aaccatatat ttcaatgtgg ggggcttata
     4381 catgatagtc gatatgcctc actcaaccga tcaactagcc cggaacttct atcaaacctt
     4441 gctggaagtc acgaacgtct taaattccca acggaatacg gaagatctgt ggaaagccat
     4501 tacgggacac attaaaaagg tgatttcttg ggaacgcgcg ggtataaccc tctatcattc
     4561 tgacatagat gcctttcggt tttacgcggt cgaaacaagt atgccggtgg tgaaattgaa
     4621 atgtgatgcc atcattcctc gagaaggtag tgcaatggga tgggtgtata atcatcggcg
     4681 tcttcatata cgtccccatt tacaacaaaa tcccgtattt ttagaagatg cattttatca
     4741 cgaagaggga cttggaagaa tgatcaatat tcccatgatt attggggata attgcatcgg
     4801 atctttgaat atcggcagcg tggaatccgg ggatcctgat ccggaaaatg ttttgttttt
     4861 acaccaagtg gcgactcaaa ttgcctttgc cattgatcat gtgcgggcct atgaagagat
     4921 ccaccacttg aaggaacagc tcacccgtga aaatgcctat cttctagaag aaatcaaagt
     4981 tacgtacgat tttggagcga tggttggaaa cagtcaaaag ttcaaaaacg tgctggactt
     5041 ggcccaggct gttgccccga ccacgacctc ggtgctcatt atgggggaaa cgggaacagg
     5101 aaaagagcta ttggcacgat tacttcatga gttgagccca cgcaaagtaa agccgtttgt
     5161 tcgagtcaat tgtgcggcgt taccttcagg tctggtggaa agtgagctct tcggccatga
     5221 aaaaggcgcg tttaccggtg cagagactgg aagacccggc aaatttgaat tagccaatgg
     5281 tggcaccttg tttcttgatg aaatcgggga gatgccgatt gaagcgcagg caaaactcct
     5341 gagggtttta caggatggta tcgtggaacg agtaggcagt atcgaaggga aagccgtcga
     5401 tgtgcgaatc attgccgcga ccaatagcga tttaagcaag gcaattgccg aaggtcgatt
     5461 tcgttccgac ttatattacc gactccatgt ttttccgatt gtggtgcctc ccctccggga
     5521 acgtctgcat gatattcccc ttctggtgcg acatttcatg gagaaaaatc ggttgcagtt
     5581 tagacgaaac tgtcaggaaa tcgatgatcg ttcaatggaa atgctggttc agtatccatg
     5641 gccgggcaat gtacgggaac tgaaaaatgt gattgaacga gccatgattc tttgccaatc
     5701 gtcggtcttg catgtggaag attcgttatt gcagtcaccg gcaaacgaca tggatgccat
     5761 gccgcccact caattaaaag acgtggagcg cactcgaata ctccaagcct tagcggcgtg
     5821 tgaatggaga attgatggac cattaggggc ggccaaacat ctggggttac atcccagcac
     5881 cctgagaagc cgattgaaaa aattcgggat caagcggtac tctgaataag acacccagtc
     5941 gtgggctgtg attgggatga gaaaccggta agtccggagg taataatttt aaataggcca
     6001 tgcccccgtc tttgtcctcc caatcgaaac ctacctcgac taacttgtga gcatacgaac
     6061 ggcttagatc aggaaaattt gtcccgtccc cccgcttcac taagatgctt tgttgccctc
     6121 aggtcattaa caggtcatcc cgacaggacg attattcaga aaggaaacct acgtaaacag
     6181 gttcctttgc accactcctg gtgagaagtc ccagcatggg aaaaaacggc tcacgccaag
     6241 gccaattttt tcagatccat gctgaaatct gtttattggt atttgactgt tcagtcgtgg
     6301 agtccgggga attcctatcc tcaatggttt aacgaagcaa atccaatctt caataagaat
     6361 ttcaatagcc cacaaattct tcagagcgaa tattggttct tggaatgccg gtagactcaa
     6421 gcatctttcg tagtccatca accattgccg ggggtccaac aacataatac aaagccgtct
     6481 ccgtctcttt aacatatttt ctgagcatga cagggtctat ccggccggtt tcacctttcc
     6541 aggtttccgt gcctccgttt gggctcgtca tcgttccaat acacgtaaaa tgcgtgttct
     6601 cttgctggag ggtttgcagt tcatcaagaa aagcggcatc ttcctgatgc cggtttgaat
     6661 acaggagaat gggatgatgt gagagtttgt gatgagcggc ttcgaccagc atacttcgga
     6721 acggtgtaat tccaattcct cctgcgagaa gaacgatcgt tcgggaatcg tcaaatggta
     6781 gcgtgaattg tccgaatgga ccttcaaggt cgattgtcgt tccaagaggc atttctgcca
     6841 acatgcgttt aaaagcggtg tcccgaagac gggtggcgac catgagccgt tgttcggaag
     6901 gagcgctggc aagggtcaag gcccgtgtat ggccttccgg atcggatggt gaaagattcg
     6961 gtaatgacag atcgataaac tgcccagctg taaaggtgaa tccagccggt cggtcaaaat
     7021 aaaacgccat tgtacccttg gcgatttcct gacgctttct caatgccacg tgataactgg
     7081 aaggtttggt gagttttccc attgaaatcg ctccttgatg gtaggatacg ttatgaggtg
     7141 ctccgaactc acattatcct agcgtttcgg tatggggttg agtcaattgg tggctcatgg
     7201 ttttcatgat ccggttaaga cgaatccatt tttccttacg gcttaaccct aaaaaaatcg
     7261 tactgccttt tcagatttcg aataagttca agcgcatttc tcttattaga caggtcattc
     7321 ggttctatgc ttgaatctgt cgcaagaaag gattgaagga ctgtggaacg aatcgagaga
     7381 aattaccggg cactcttgaa tgtcgcgatt gtgctcaatt cacaacggga tacagatagt
     7441 ctctggcaag ccattattga gcagattgaa caggtcatgc cctgggccag ggcaagtgtg
     7501 acgctatatg accgtgagtc tgacgggttc agattttatg tggtgacgac tcatttggcg
     7561 aaggtcgtgc ttcaaggtga ctcagtgatt ccgcgggtag ggagtggcat ggggtgggtg
     7621 tacgaccata aatctatgca tgtccgtcct gatctcagac aggaacaggt ctttctggaa
     7681 gaccaatggt atgtggagga aggactggga cgcatgatca atctgccgtt gttggtcaga
     7741 ggaaagtgta tgggggtgtt gaatcttggg agcgttgatc ccggcaaccc ggagccggat
     7801 gcgcttgagt ttttatccca agtggctatg caaattgcct atgcgattga tcatgtgcag
     7861 gcctatgagg aaattaatct gctgcgtcag cagttggcac gagaaaacat ctatctggtc
     7921 gaggaactca agctgacaaa acacttcggg gccatggtag gacgaagtca ggtttttcag
     7981 catgtattgg aacttgcccg cgaggtcgct cctacaccgg cgacggtcct gatcacgggg
     8041 gagacgggaa ccgggaaaga actcctcgcc caagccattc atgattggag catgcgtgcg
     8101 aacaaaccct tcattagggt caattgtgcg gcccttccgg caggattagt cgaaagcgaa
     8161 ttatttggtc acgaaaaagg cgcatttacc ggagccgact cgaggcgtga ggggcgtttt
     8221 gagttagccc atggcgggac cttgttgtta gacgaaatcg gggaaattcc tttggagacc
     8281 caggcgaaat tattgcgagt gatccaggac gggatggttg atcgtgtggg cggcaaacag
     8341 cctatcccgg tggatattcg gttgatggcc tcgaccaatg ccgatctgcc atcagccatt
     8401 gaacaaggtc gttttcgtgc tgaccttttc tatcgacttc atgtgtttcc gattaccatc
     8461 cctcccctcc gtgcccggcc ggaagatatt ccagagctcg cacagcattt tctggagcac
     8521 tatgctatga aactcaagcg gccctgcgaa acgattgaac cggattctct gcagcggctt
     8581 atccggtatt catggccggg caatattcga gaactggaaa atgtgattga acgggcggtg
     8641 attctctccc accaaaaggt tctgcgcatt gatgaacggc ttttgattct tcagaatgcg
     8701 acgcatgaat caaaggctcc ggccggttta gttgattttg aacggcaaca tattcttcag
     8761 accctggcct ccagcaattg gcagatcgag ggggagggcg gcgcggcgga acaactcggc
     8821 ttggcaccca gcacgctcag gagccggatc aaaaaattag gtctacaacg accgagtagc
     8881 tcaacccctc caaaatagtc tgtggtgatt cctttctcta taaaaatccg acttcactcc
     8941 tagggccggt tctccacgat gtgtcgtggc tgtaggccac gacactccac ctaattttcg
     9001 tggatcaaaa aagaccttct ctgttaaaca gaatataaca aattggtttt attgatgagt
     9061 ttctcctaat cggatatctc gcacaatggc ataggggatg cattgttaaa agtaggtaaa
     9121 gaaaatttca ataaaaaaag gagttcccaa tgactatctc tagaacacaa agtcgagtga
     9181 tttctgagcg taggacatta ctgcctgtga gcgttttcgc ggcctccttg ttgttcttga
     9241 taggtgtttt gattccggtc acttcaaaag ccgctgaaac aaatgatgga catcatgaat
     9301 tgctctccgg agaacggatt cttcttgcga cggtcgagga aaccagcagc gctgatgtca
     9361 gggtcgatac cggcgatttg cagccccgtt atcttccttt gaacgtacgt aaggacaagg
     9421 ggttgccgga actccaggtg ggggatcgca tagccattac ggtcaacgac cagaatctga
     9481 tcgtcgatgt acatttgctc ggggaaattg accaccatca aatcatacgg ggtgtgctgg
     9541 cccaaccgct catgaccggg catgaacagg cggtgattcg aacggaagat gggaaggagg
     9601 agtcttacgt cgtgaggcct ctcgccagga gcaaggtggc gtccgttcca gtaggtgccc
     9661 acgcgatctt cctccttgat gaacggcacg ggattgccga tgccacctat gagagcgccg
     9721 aggcggccgc ggaggctcag cagcttaggc aaaaaaggtc gcccctcaaa tccagtttta
     9781 agcgagtgac aggcgtcatc ctcaaaacct tggatcacaa tacgatctcc tttcaggctc
     9841 atgagggttc tgttcagctg cttgaggtca gtccgcttgt tctggataag ctgcccaagc
     9901 tgcctaaagg ccacttggtg gtgctctttt tggatgccga caatacggtc accgatgtgt
     9961 cggcctatga tagggaacat taacgggaga cttttcgagt gtacagagaa acttgaagat
    10021 gagggggagg gaccatgctg acacagggct ttccggtgga agggttaaac acggtcggac
    10081 aaatcgtcgc gaccaacacc gtacggtttc atgctcaaca gaacggtttg gacatcgcag
    10141 gagcactact ttcgacgcat atggccggtt cgccggtggt ggatgaccaa gggcgctacg
    10201 tcgggtttat taatgagttc gatgctatgc gagcgctggc ggcagggaac gatctcggca
    10261 agctcgtcgc gaaggacatc atgcgcaaag atcaattagc cgtgatatcc tcaactccga
    10321 ttgaagaggc cgccaagcgg atggaagagt atcatgtttt caacctacca gtgatcgacc
    10381 aatatggggc tgtggcctat tcagtctcgt ggcatgatct tttgcgggcg tggatcggcc
    10441 cgggggttga ccctggagtc gcgggcctat cgttatgggc tacgaacagg ggagaataac
    10501 gggcggtgtc tcactcggtt cgcacgtgag gcatgttttg agataaatca gtaccccgaa
    10561 gcaattgaat cggagaaagg tcctgtttat ggtgagtctt cagaaactta tacagtacca
    10621 attaagcgaa ggcctaacgg aatatgagtt ggcagaagac atcggtgtgc ccaagtttac
    10681 gattcagagg attctgaggg gcagagatcc ggagagtctg gatatctgga agaaattggc
    10741 gcgctacttt catgtggata tggaacttct ccgttttggt cattttgcct tcgtacatcc
    10801 accaatgaaa gacgaagatc gatctgatgg tcctcagccg tatcgaaagg tgccgatact
    10861 ctcatggact caagttcgcc atgtcgtgga aggccacgac tcaaaatttt tcttggctgc
    10921 tggacagggg atggtagaaa ctgaactcac gggagcgcgg gtatttgcaa tgcatgttga
    10981 ggatgattcc atggagcctc tgttccatgt gaaggaaatc atctttgtga atccggacct
    11041 tcggccccag tctggcgatt atgtggtggt cttggatcaa atggctgagg cggatcatgg
    11101 ttgtcttagg cagctcaaac ggattcagaa gcaatatatt ttgcaaccac tcaaccttca
    11161 atacaaggat gtgaatctgg cgagtcatca gaaaatcgtt gggcgggtgg tccggcttcg
    11221 gatgaatctt taatcgcgtt gttcggatgg gattgctgcc agtcgcgagg cgcggattat
    11281 tcgggaaatg ggggctgacc agctgacatg atcgaacggg gtgaagggtc agtcgaaaag
    11341 tgaaatcaac aatctgtgtc ccctatcatg atatggtgca gccacgtgag cataacgaca
    11401 gcagaggtga accatgacta cccgggaacc gtctttaaca gtttatgacg tcctcatcgc
    11461 cctaggacaa ttccagggtt tgtctgcgaa cccgaagctt atcagttctc ctttattggc
    11521 cgcattaaaa gacgcgggaa cggcacatat atatgccgat acggcaagcc gggaagaatt
    11581 aatgacggtt ttgacggttg atcaggagac catacttaaa gaaattgatg gcaacaccgt
    11641 caaccagccg ctgctggcta aggtcatcaa acagtatctc aaacaggaca acccagccaa
    11701 atgggcacag caccttcgcg aaaaagagcc ggaactttcg cagggggacc tcctcccgtt
    11761 gttctacagc attgtctgcg ggaagatcgg caccgacatg gtgcgggcct ttgcgagcgg
    11821 tcgttcgtgg gaagtgagcc tacagctcca tatgggattg gtacacaatt tggaaaatgc
    11881 gaagcagatc ggccgttcgt tgcgacgaat ggtcccgagt gccttcgtaa aagttccgtt
    11941 tgcgcctcat gcgccgatgt gttttttaat tgcgcgggac ctggagaagg aaggcattcc
    12001 ggtgaatttt acctccacgt tttcagcgag acaggtcgtg gctgctgcgc tgttgtccaa
    12061 cgtcactcga acgaatattt tcatgggacg gctggaccag gggctcaaag ccgagctgct
    12121 cggcgcacac gtgtctttgg aagcacagcg gatactcctg cggctcaggg gaaattccgg
    12181 aatcaaaact cagctaatcg tcgcgagttt gcgggactgg gggagtttac tccataccgc
    12241 ggggtgcgat gtctttaccg taccgtgcaa ggttcttcag gaatttctcg aacagaatga
    12301 agtgaaagac agttccatcc agagtcgcct cggcacttcg tacgaagatc gcctcggaat
    12361 tccggacaac attgtcgcga agttgggagc tgatcgtatc gccaggctat acacggtcga
    12421 acccgaattc gtggattttc ttcttgagta ccgggtgacc accgaatatc aagatctgga
    12481 tgacggcgat catttggtgc gacggtttga acaagccgga ttcggagatc tgttctatgc
    12541 ccccgatcat cacgaatgga cggcattgcg acgaggaaaa gttccggacc tggaagatcc
    12601 aatcacgatg aggccgtcct tggacacctt gtacagcctt cttgccgatg cggactttga
    12661 gaaatatcag gaggacatgg atcgggaaat gacgcggtat ttgtagaaga caacgtagca
    12721 ttcattcctt gaaaaggtta agccatgata cgaatcaaac gtgtctatcg ggagccgagt
    12781 tcgcgcgatg gga
//
LOCUS       DATIAL010000393         2458 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_717297, whole genome shotgun sequence.
ACCESSION   DATIAL010000393 DATIAL010000000
VERSION     DATIAL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717297"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            372..>2458
                     /locus_tag="VLA60_12220"
     CDS             372..>2458
                     /locus_tag="VLA60_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10173.1"
                     /translation="MFHMKSRKAITTLSIGLLVGGSSFLSGGTLLAEENQDKVIHSTG
                     MVHQESPGWAERLKGQTIREDIQEGHPERAAMVERQHDRIMLQMNSESDGQSHASHTS
                     SGFYNTMTNMHQYGAGGQDLLLASDRNVEPVAASGGNCPSTAPVREYDISAINAEITL
                     NQWLDYYPGYMYVLTENIDKVREEEAKNAAAREVDGHGDPGAVTNGLQDQWIQPLVIR
                     GNQGDCVKFTFRNQLEFGEEASLHIHGSNMVISKTGQPATTTNRDTVAEEGQVVEMEW
                     YIHPDTQEGGRQFHTFSNDRELTVMGLMGTFVVEPHGSDYLNPLGNGDPTPMASGWQA
                     IIKNGTGPDFREFVIIYHEVGDEAFRPVNKHGDFLPQRDPLTDTYRPGARAINYRSEP
                     FGINMMHVQHEYFGFEDESMGYSSYTFGDAPTTIPRSYLGDPAKYRLVHGGSEVFHSH
                     HPHGGTIRWPRSPRAIDEMPMWHAAKNGPVKYPVIRTKTDRVDVEVIGPSEALDLETE
                     CGSGLCQQLAGDFLFHCHVAHHYVSGMWGYWRVYNTLQTEDNHTDVMPPLRELPDRKG
                     RMKVGVTSEQLIGKTVDWFGKKFTIVEKGKTNWKADPAVVNIKEWVEIQLPTQGKPGH
                     KDDERGQILSYDATVLDWAWDGNKALSEKESTIENPKYASKTPGKRRPILFEPTTGKV
                     SWPHLTPHFGKRV"
BASE COUNT          670 a          521 c          647 g          620 t
ORIGIN      
        1 aacaactcat acaggatgga aagccaggcc ccatttccac tgatcttcaa aaggagtttt
       61 cttcgtatgt tgcagggctc accatgtcgt agctttttaa aatcggaaat ttaaaaggct
      121 ggagtgaatt attagccgtt aactcggtga caatttacct atctcccaca ttcatttcgg
      181 gtatcccatt ttttatagag tagttgtgag aaatgcaatg aaatcaaact catatctttc
      241 agacttgaca gtctcaatcg attggattac cttttgcgct ctccaacatt ttcatggaat
      301 attatcgact gtttgatttg tagaataagt ctttggtttg tcagtttatt tcaccttaag
      361 gaggccggtc catgtttcac atgaaatcta ggaaagcgat caccacactt tccattggat
      421 tgttagtcgg ggggtccagt tttttgtcag gaggaacact cctggctgaa gaaaatcagg
      481 acaaagttat tcacagcacg gggatggttc atcaagagtc tcctggatgg gctgaacggt
      541 taaaagggca gaccattcgt gaagatattc aggaagggca tccagaacgg gccgcaatgg
      601 tcgagcgtca gcatgaccgg atcatgcttc aaatgaacag tgaatcggat ggtcaatccc
      661 atgcctccca tacttcttcc ggtttttaca acacgatgac caatatgcac caatatgggg
      721 cgggaggcca ggatttactt ttggcatcag atcgtaatgt ggagcctgtt gcagcgagtg
      781 gtggaaattg tccttcaact gcccctgtca gggaatatga tatttcggcc atcaatgccg
      841 agattactct caatcaatgg cttgattact atcctgggta catgtatgtc ctgacggaaa
      901 acatcgataa ggtacgggaa gaggaagcca agaatgccgc agcgagagag gtggatggcc
      961 atggtgaccc tggtgcggtc acgaacggtc ttcaggatca atggattcag cctctggtga
     1021 tcaggggaaa tcaaggggat tgtgtgaaat tcaccttccg taaccaactt gagtttggtg
     1081 aggaggccag tcttcacatc catgggtcga atatggttat cagcaagact ggtcagcctg
     1141 ctacgacaac caatcgcgac acagtggctg aagaaggaca agtcgtagag atggagtggt
     1201 atatccatcc ggacacacag gagggtggcc gacaatttca tacttttagt aatgatcgag
     1261 aattgacggt catgggtctc atggggacat ttgtggttga accgcatggc tccgattatt
     1321 tgaatccgtt aggaaacgga gacccaaccc ctatggccag tggatggcaa gcgattatca
     1381 aaaatggaac gggaccggat tttcgtgaat ttgtaatcat ttaccatgaa gtgggtgacg
     1441 aagcgtttcg tccggtgaac aagcacgggg atttcttgcc gcaacgggac cctctcactg
     1501 atacctatcg ccctggggct cgtgctatca attatcggag tgagcctttc ggtatcaata
     1561 tgatgcatgt gcaacatgaa tacttcggtt ttgaagatga atctatggga tatagttcct
     1621 atacctttgg tgatgcacca acgacgattc ctcgatccta tcttggggat cctgccaaat
     1681 acagactggt tcatggagga tctgaagttt tccattccca ccatccccac ggtggaacga
     1741 ttcgatggcc aagaagcccg cgggcgattg atgaaatgcc gatgtggcat gcagccaaaa
     1801 acggtccggt taaatatccg gttatcagga caaaaaccga ccgggttgat gtagaagtga
     1861 tagggccatc agaagcgttg gatttggaga cggaatgtgg atccggctta tgccagcaac
     1921 tggcgggtga cttcttattt cattgccatg tagcccatca ctatgtttca ggaatgtggg
     1981 gatactggcg agtgtacaat acactgcaga cggaggataa ccatacggat gtcatgcctc
     2041 ctctcaggga attgcccgat cgaaaaggcc gaatgaaagt gggtgtgact tcggaacaat
     2101 tgattggaaa gacagtcgat tggtttggaa agaaattcac tatcgtggaa aagggaaaaa
     2161 ccaattggaa agccgatccg gcggtagtga atatcaagga gtgggtggaa attcagttgc
     2221 caactcaagg gaagcctgga cacaaggacg acgaacgtgg ccaaattctt tcctatgacg
     2281 cgacagttct ggattgggcg tgggatggca ataaggccct gtctgagaag gaaagtacaa
     2341 ttgagaatcc caagtatgca tcaaaaacac ctgggaaacg gcgaccgatt ctgtttgagc
     2401 ccacgacggg aaaggtgtca tggcctcacc tcaccccaca tttcgggaag cgggtcat
//
LOCUS       DATIAL010000394         5486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_610226, whole genome shotgun sequence.
ACCESSION   DATIAL010000394 DATIAL010000000
VERSION     DATIAL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610226"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VLA60_12225"
     CDS             <1..460
                     /locus_tag="VLA60_12225"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HSF10174.1"
                     /translation="FRFFQVILMLSLTVFAPPDLAKSWGATPVSLAPAADPQETVVTI
                     NIKSREFSPNTLSITVGQKTRLVLKNLDSELHAFLPIGLLTDIHLNVSGSGAPQFSKE
                     GLSRVLLPTRGQTDIVFIPSHPGRFPYFCDLPGHVMQGTIVVHKNEGVVD"
     gene            541..2304
                     /locus_tag="VLA60_12230"
     CDS             541..2304
                     /locus_tag="VLA60_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10175.1"
                     /translation="MSIVRRQPIMTNTLKPEADHRLGSPVHRMWPTLKRVGLLCGSVL
                     LASTHVWAAEPASVNDEALLSTVREYANAVGKGDQVAAGQRDFVCLHQMAQQQLFSNG
                     NFPDPSNPIYEWCDQRRQEVHQRAIQQNDRALDNVWPGPGKLVDFSDFERFFIAETLN
                     QQLAPSFFVMKAIAVQEPTNPFIIEAVESGTLPHASFPSPDETRVVAAPTAFLTTKVS
                     YPNALTAPIANAPGAEDWAVPYKKAQRVVKSVNVKWVVLSDLKQLGFPTDRAVLDLVL
                     DGPRGTIIPFVVDPGGYVPSSTEWFGLPESLAAIQTGIQQAQAAPTRLESVMRLNRVL
                     LIGPSHKPALEVFSDQLYKGFLDYGGRLNGLQLGDERLAQRFNELYWTVQSQTDRFDL
                     SLGMEIGGKSEPMPADYLYRMIPVMEALASLEPGDFNNRIRLSSTYRWTNDQMAALSA
                     PQELLTEVPAEQEELRAQVLLELAWARIGKVAWNRHFDDPDIQRGYEAAQKAFELTKD
                     PLNKFTAAYAMAYSLAFHVPRDNQAMLGLLQQARDWFEKTPGSSPQSWAYMLHNDTLK
                     GLVENDPAFKSLLAAQLEPTK"
     gene            2463..3848
                     /locus_tag="VLA60_12235"
     CDS             2463..3848
                     /locus_tag="VLA60_12235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10176.1"
                     /translation="MELDFRKNPAILSNMVDVMADGVFTVDAKGHIVAWSTGAARITG
                     YSSQDILGKSCHILEGQNCKGFRILTEFLDNPTPYPWGICNQECKVLGKDGRELYLFG
                     NVSIIRDEQGQVGGAVGTFTDLTSFILNNQKIAVLEEQAKSRESFQQLIGKSPAMQEV
                     FRRLRLAAQSDVTVLLTGESGTGKELAARAIHTLSDRNDKPFFAINCSAIPETLLESE
                     LFGHVKGAFTGAIRDKIGVFQAADGGTLFLDEIGDTSPLLQLKLLRVLQESEIRRVGD
                     DRGMRVDVRLITATNRDLKALMAEGTVREDFYYRIHVFEITLPPLRARREDIPLLVHH
                     FMAANSKTFGRKIGDIAKDALQRLTDYNWPGNVRELKNAIEHAFVTVNSDCLTLWDLP
                     SEVQSPPRSRSLKPLSANTEPSLPPEEEARIAEALQQTKGNKTKAAKLLGVSRVTLWK
                     KIKQLQTPPNA"
     gene            4117..>5486
                     /locus_tag="VLA60_12240"
     CDS             4117..>5486
                     /locus_tag="VLA60_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF10177.1"
                     /translation="MIVKQFYLSCLAHASYMIGDNKTSTAVVVDPQRDIEQYLQEARR
                     HDWKIRYVFLTHFHADFLAGHLELQRQTGAEICLGAKAKTDFPIRNFRDKEVLEFGSV
                     RIQVLETPGHTPESISLTVYDLDKSTDHPHCVLTGDTLFIGDVGRPDLMASIGVTAEE
                     LGGQLFDSLRNKLMCLPDETLVYPAHGAGSMCGKNLSSDTVSTLGRQRWENYALQPMT
                     KEKFIELVTADQPEAPSYFGYDARMNREQHPVLNDVVRNSLTPLSLQAVLDHQQNGAQ
                     VLDVRNAVEYAGGHLKGSLNIGLGGKFATWAGTVLNPKSSIVIIAEPGYEKEAIQRLG
                     RIGFDRVLGYLKGGMQSLEVNPDVVQKVQRITAQGLAELLTTPHPPMVVDVRSVKEWE
                     SGHIDGSLNIPLPHLAEQVQDIPADTPVVVHCASGYRSSTAIGILEQAGRTQAMDLVG
                     GYDAWLT"
BASE COUNT         1363 a         1444 c         1378 g         1301 t
ORIGIN      
        1 gttcagattt tttcaggtca ttctgatgct gagtctcaca gtatttgcac cccctgactt
       61 ggctaaatca tggggagcga cgccggtcag tctcgctcct gctgctgatc cccaagaaac
      121 agtcgtgacc atcaatatta aatcccggga attcagcccc aacactctat caatcaccgt
      181 ggggcaaaaa acccggctgg tcctcaaaaa cttggattcg gaactgcatg catttctgcc
      241 aatcggccta ctgacagata tccacctgaa cgtcagcgga agtggtgctc cacagttttc
      301 caaagaaggt ctcagccgcg ttttgcttcc cacccgaggc caaaccgaca ttgtttttat
      361 cccatcgcat cccggtaggt ttccctattt ttgcgattta ccgggccatg tgatgcaggg
      421 taccatcgtg gtgcataaga atgagggtgt ggtagattag ccacctggat tatgagggac
      481 attcccctct ctgctacaca aaatcattaa ccaagcatgt ttgaggagga gtttggttcc
      541 atgagtattg ttcgtcgtca accaattatg acaaataccc tgaaacctga ggcagatcat
      601 cgtctcgggt cccctgttca ccggatgtgg cctacgctta aacgagtggg ccttctgtgc
      661 ggttctgtgc ttttggcctc cactcatgtg tgggccgccg agccagcgtc cgtcaatgat
      721 gaagccttgc tctccaccgt tcgggaatat gccaatgcgg tcggaaaggg ggatcaagtc
      781 gcggccggcc aacgggattt tgtgtgttta catcagatgg ctcaacagca gttgttttca
      841 aacgggaact ttcccgaccc gtcaaacccc atctatgaat ggtgcgacca gcgtcggcaa
      901 gaggtccacc aacgagccat tcaacaaaat gaccgggcat tagataatgt ctggccggga
      961 ccgggaaaac tggtcgattt ttccgatttc gaacgatttt ttattgcgga gacgcttaat
     1021 cagcaattgg ctccatcgtt tttcgtcatg aaggccattg cagttcaaga accaacaaac
     1081 ccatttatca ttgaggccgt ggaatcggga accctgcccc atgcctcatt tccttctcca
     1141 gatgaaacca gggtggtagc cgcacccaca gcctttctca ccaccaaggt gtcctatccg
     1201 aatgccttga cggcacccat cgccaatgct cccggcgccg aggattgggc cgtgccctac
     1261 aaaaaagccc agcgagtcgt aaaatccgtc aacgtgaagt gggtcgtgct ctcagatctg
     1321 aaacaattgg gttttcctac ggatcgtgcc gtgctggatc tggttctcga tggcccacgt
     1381 ggcaccatca ttccattcgt ggtggacccg ggtggctatg ttccgagctc aacagaatgg
     1441 tttggccttc cagagagcct ggcggccatc caaacaggca ttcaacaggc ccaagccgca
     1501 ccgaccagac tggaatccgt aatgaggctg aaccgggttt tgctgattgg cccgtcccac
     1561 aaacccgcac tggaagtttt ttcagatcaa ctgtacaaag ggtttttgga ttatggaggt
     1621 cgtttgaacg gccttcaatt gggtgatgag cgtctggccc aacggttcaa cgaactgtat
     1681 tggacggtgc aatcccaaac cgatcgattt gatctatccc tgggaatgga aataggcggg
     1741 aagtctgagc cgatgccagc tgattatctc tatcgaatga tcccggtgat ggaggcatta
     1801 gcatctctgg agcccggtga tttcaacaac cggattcgat tgtcctcgac ctaccggtgg
     1861 acgaacgatc aaatggcggc cctgtccgct ccacaggaat tattgactga ggtgccagcc
     1921 gaacaggaag agcttcgcgc acaggtgctc ttagagttgg cgtgggcccg aatcggaaaa
     1981 gtggcctgga atcggcactt tgacgatccc gatattcaga gaggttatga ggcggctcag
     2041 aaagcgttcg agctcacgaa agatcccctc aacaaattta cggcagccta tgcgatggcc
     2101 tatagcctgg cgtttcacgt tccccgtgac aatcaggcga tgctgggtct cctgcaacag
     2161 gcccgtgact ggtttgagaa aactcccggc tcaagcccgc aaagctgggc gtatatgttg
     2221 cacaacgata cgctgaaggg gttagtcgag aatgatcccg ccttcaagtc attgctggcg
     2281 gcacagttag aacctacaaa ataggtgcgt gaggccttat gaaatttcgg acaaactaac
     2341 gactaccaag gctggagaat gttcattgaa acaataccat tctccagccg tcttggttca
     2401 cctaccctct ttttttcaac ccgctcaatc tcacccggtg aactttcttt gaatcacaga
     2461 ctatggagtt agatttccgg aaaaatcccg ccatcctcag caacatggtt gacgtgatgg
     2521 cggatggcgt attcacggtc gatgctaaag gtcacattgt agcctggagc accggggcag
     2581 ctcgcattac gggatattcc agccaggata tcctgggaaa atcctgtcac atcttagaag
     2641 gtcaaaattg taagggcttt cgcatcctga cggaattttt ggacaacccc accccgtatc
     2701 cttggggcat ctgcaaccag gaatgcaaag tgctggggaa agacggccgc gaactctacc
     2761 tcttcggcaa tgtctcgatc atccgagatg aacaaggcca ggtcgggggc gctgtcggaa
     2821 cctttaccga cctcacctca tttattttga ataaccaaaa aattgccgtg ctggaggaac
     2881 aggcaaaatc ccgtgagtcc tttcaacagc tcatcggcaa aagcccggcc atgcaagagg
     2941 tgtttcgccg gctccgcctg gctgcacaaa gcgatgtcac ggtattgctt accggggaat
     3001 ccggcaccgg aaaagaactg gcggcccgag ccattcacac cctgagtgat cgaaacgata
     3061 aaccgttttt cgccatcaat tgctcagcga ttccagaaac cttgcttgaa agtgaactct
     3121 tcgggcatgt aaagggggcg ttcaccgggg ccatccgcga taaaatcggg gtctttcaag
     3181 ccgccgacgg tggcacgtta tttttggatg aaatcggaga taccagccct ctcctgcaat
     3241 tgaaattgct cagggtcctg caggaaagcg aaattcgccg ggttggagat gaccgtggca
     3301 tgcgggtcga tgttcgtctc attacggcca ccaatcggga tttgaaagcc cttatggcag
     3361 aaggcacggt acgggaagat ttttattatc ggattcatgt gttcgagatt acccttccac
     3421 ctttgagagc cagacgagaa gacattcctc tcctggttca tcatttcatg gcggccaact
     3481 ccaagacgtt tggtcgcaaa ataggagaca ttgccaagga tgccctgcaa cgattgactg
     3541 actataactg gcccggcaat gtccgcgaat taaaaaatgc gatcgaacac gccttcgtca
     3601 ccgttaatag tgattgcctc accctgtggg acctcccttc agaagttcag agtcctccaa
     3661 gatcccgaag cctcaagcct ctctcagcca acacagagcc ttcccttcca ccggaggagg
     3721 aagctcgaat cgcggaagcc ctccaacaga ccaaaggcaa caaaacaaaa gccgccaagc
     3781 ttcttggagt gagtcgagtc accctctgga agaaaatcaa acagcttcag acccctccca
     3841 acgcataatc aaatcgttaa cacgttaact taacagttag cgcattttgt tcaggtcaac
     3901 tgcctttaag gccgtaatca ggcctttcct catttcaaca aatccaataa ttaattgatt
     3961 ttattgaaaa ttttcaaatt cgtctgccaa gcataatctg gcacagtcca tgcgtaatga
     4021 tgttacatgg aaagaatctt acaagaatat ttcaaaacga gttcaatcga tttcaaccaa
     4081 tgctcctttt tcaagaagga ggggagggta ttcatcatga ttgtgaaaca attttatttg
     4141 agttgtttgg cccatgcgtc ttacatgatt ggcgataaca aaacctcaac cgctgtggtc
     4201 gtggatcctc aacgggacat tgagcaatat cttcaggagg cccgacgcca cgattggaag
     4261 attcgttatg tgtttctcac ccacttccat gcagactttt tagccgggca ccttgaactc
     4321 caacgacaga ctggcgctga gatctgcctg ggcgccaaag ccaagaccga cttccccatt
     4381 cgaaactttc gggacaaaga ggttcttgag tttgggtcgg tgcgcattca ggtgttagaa
     4441 actccggggc atacccccga aagtatttcg cttaccgtct acgacctgga caagagcacc
     4501 gaccatccgc actgcgtgct gacgggagat actctgttta ttggcgatgt gggcaggccg
     4561 gatcttatgg cctccattgg tgtcaccgcc gaagagttag gggggcaatt attcgattcg
     4621 ctccgcaata aactcatgtg tcttcccgat gaaaccctgg tctatcctgc acacggagcg
     4681 ggatccatgt gcgggaaaaa tctaagcagc gacaccgtct ccacattggg caggcagcgt
     4741 tgggaaaact atgcacttca acccatgacg aaagaaaaat tcattgaatt ggttacggct
     4801 gatcaacctg aagcgccttc gtactttgga tatgacgcaa ggatgaatag ggaacagcat
     4861 cctgttctga atgatgtggt gcggaacagc ctcacgcctc tttccctcca ggctgtcctg
     4921 gatcatcagc agaatggagc tcaagtcctt gatgtcagaa atgccgttga atatgcggga
     4981 gggcacctga aggggagtct caacattgga ttaggaggaa agtttgcgac ctgggccgga
     5041 acagttctga accccaaatc ctccatcgtc atcattgcag aacctggata tgaaaaggag
     5101 gcgattcaac gattaggccg cattggattt gaccgcgtcc ttggctacct gaagggtggc
     5161 atgcaatccc ttgaggtgaa ccctgatgtg gtccaaaaag tccaacgcat cactgctcag
     5221 ggacttgcgg aactattgac gacccctcat ccccccatgg tcgtcgacgt gagatcggta
     5281 aaagagtggg agtccgggca tattgacgga agcctcaaca tccctttgcc tcacctggcc
     5341 gaacaggttc aggacatccc tgcagatact ccggtggtgg tccattgcgc cagtggatat
     5401 cggtcatcta ccgccatagg gattttggaa caagccggac gaacccaggc catggatctg
     5461 gtcggtgggt atgacgcctg gctcac
//
LOCUS       DATIAL010000395        15071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_72856, whole genome shotgun sequence.
ACCESSION   DATIAL010000395 DATIAL010000000
VERSION     DATIAL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15071
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72856"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1669
                     /locus_tag="VLA60_12245"
     CDS             110..1669
                     /locus_tag="VLA60_12245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HSF10178.1"
                     /translation="MNSPNNTDSLHPLEIQVLSELGKYPQPLSDLQLANHTELAPSQV
                     SMAVGWLLTKQLVSLSSETTTTYVSLTSVGAQYHQPNSSPLEWILQTVKSAAHDGSSP
                     TVKDLQGTGKFQPSELSRAIGILKKEGVLQMGAGGVLEITSKDSDTMRDLRSLLNQVQ
                     EGARPLDSFASDHQALLRQYAVKRGNTQEPFRIDDHTERTYVLTDEGRNLLPHLREGA
                     AQEISQLTPELLKDGSWRHVSFRKYSINLRPPRLAVGRRHSYREFLDTVKYKLTSMGF
                     QEMRGELIETEFWDMDALFMPQFHPARAIHDVYFVKNPKMAKKIQEPFLSRVGQVHRD
                     GGTSGSKGWKYEYDPERAKRLVLRSQGTAVSARTLAQNPPVPGKFFSIARCFRYDNVD
                     ATHATDFFQVEGIVLGSDINFRTLLGLLQLFATEMAQAKEIRFFPAYFPFTEPSVELH
                     VRHPKLGWMELGGAGLFRSEVTTPLGVTVPVIAWGLGLDRMAMIALGIQDIRDLFTSD
                     LEAVRNMRRHF"
     gene            1679..3373
                     /gene="pheT"
                     /locus_tag="VLA60_12250"
     CDS             1679..3373
                     /gene="pheT"
                     /locus_tag="VLA60_12250"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HSF10179.1"
                     /translation="MPTISIFQKDFCRLVGRDASTSDIEQWMPYVKGEVKDVSQETGE
                     VRVELQDTNRPDLWCVEGIARQIRSVLNKGMPPYSFFSEKRGAKRRIQVAQGMEVVRP
                     FVAGCVSLGYAMTPDGLDQCIQTQEKLADAFGRKRETVSIGLYRASAIAFPVTYGLVK
                     PDEIRFTPLGFEEKMTPQEILTVHPKGLEYGSILAGCERLPLLWDSDGQVLSFPPIIN
                     SRELGEVQLGDTELLVEVTGTDLSMVVLALNIFACNLADRGATIESLDINYPYKTEFG
                     ATVKTPLSMNQSQRISIEAIEQALGMPLGSEVIQEALASYGYQVKATRQSVSVTLPAF
                     RNDFMHVMDVAEDVAITRGYDSFSPIMPQTFTVGSLSLEEQTSDRIRDLLVGFGFQEI
                     FSNIMASHQELVDRMRIADTEYARLVEVDNVMSQTYACLRPSILPCLLRVEALSPRAF
                     YPHLLFEVGETAQLDLEANVGSRTTLSVAAISANSGANFSEIHSYLDLLMYYMAWPYE
                     LEPVTHPSFLEGRVGRIRCEGYAVGYIGEFHPEVLEAWQIDMPTSGFEFEIRKPEA"
     gene            complement(3370..3738)
                     /gene="rplT"
                     /locus_tag="VLA60_12255"
     CDS             complement(3370..3738)
                     /gene="rplT"
                     /locus_tag="VLA60_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HSF10180.1"
                     /translation="MPRVKGGPQTRQRRKKRLKLASGQYGGKSKLFRTATESVDKGQA
                     YAYTGRKQRKRNFRQLWVTRISAAVRAQGVTYSQFMNALKKANVLLNRKMLSEMAIHD
                     PQGFSHLVTLTKGDGQPVAA"
     gene            complement(3766..3969)
                     /gene="rpmI"
                     /locus_tag="VLA60_12260"
     CDS             complement(3766..3969)
                     /gene="rpmI"
                     /locus_tag="VLA60_12260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HSF10181.1"
                     /translation="MAKMKLKNHSGASKRFKRTGSGKWMRRKAGMRHILTSKAPGVKS
                     RLSGSAEVRKEDRTSLSRLLPYK"
     gene            complement(4027..4533)
                     /gene="infC"
                     /locus_tag="VLA60_12265"
     CDS             complement(4027..4533)
                     /gene="infC"
                     /locus_tag="VLA60_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSF10182.1"
                     /translation="MTPKQRINHFIKNPEVRVIGAEGEQLGILKTSEAIRQAREIGYD
                     LVEVAPTADPPVCRIMDYGKFKYEQSKKEHRMRLNQKSTQVKEVKFRPRTDKHDMETK
                     VRQIRDFLEEGNKTKVTVMFRGREMANQELGFKAIQKVIEELKGAGNIESPPRMEGRN
                     LYMVVAPK"
     gene            complement(4542..6485)
                     /gene="thrS"
                     /locus_tag="VLA60_12270"
     CDS             complement(4542..6485)
                     /gene="thrS"
                     /locus_tag="VLA60_12270"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSF10183.1"
                     /translation="MESIQVSLQGESSQALPKGISAKAALEKLKGAVPPEVFAVKAND
                     VEIDLLASLETDSTLQPLMFDSPEGKEIYRHSSTHIMAQAVKECFPSAQLTIGPAIEE
                     GFFYDFAFERPFTPEDLEKIEAKALEIIKRNLPVSRREFSKEEAIEFFKNRGEFYKVE
                     LIQGFPDGEPVSAYTQGDFVDLCRGPHLPATGYVKAFKILNSAGAYWRGDERNPMLQR
                     LYGTSFPSREALQAHLDNLEEIKRRDHRKLGKDLDLFSIQDETGPGLILWHPKGAQIR
                     LLIENFWREQHLANQYELVYSPHVARLDLWKTSGHVDFYKENMFASMPVESSEYQLKP
                     MNCPFHIMVYKSHLRSYRDLPIRYGELGTVYRYERSGVLHGLMRVRGFTQDDAHLFCR
                     SDQLGEEVQKVLKFITSMLGTFGFTEFKMFLSTRPEKAVGTIEQWDQATLALETALKS
                     GGYTYQEDPGEGVFYGPKIDVKIQDALGRSWQCSTVQIDFNNPERFGLTYVGDDGKTH
                     QPIMIHRALLGSIERFFGILLEHFGGAFPAWLAPVQVTVLPISEKHQGYAQEVEKNLR
                     QEGCRVVLDLRNEKIGLKIREAEKAKIPFMIVVGDREAEAKMVAVRQRNGKNLGTMPI
                     IDLVTLIREDMPEAVRKGSLLFE"
     gene            complement(6603..6677)
                     /locus_tag="VLA60_12275"
     tRNA            complement(6603..6677)
                     /locus_tag="VLA60_12275"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6643..6645),aa:Val,seq:gac)
     gene            complement(6759..7373)
                     /locus_tag="VLA60_12280"
     CDS             complement(6759..7373)
                     /locus_tag="VLA60_12280"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF10184.1"
                     /translation="MNPSHIRFALLTQDPDLKSRVQGPDLQNTITVLDDCSSLEAAMR
                     SQQFTGVILDESGKKFFPALSALNGQLNLSKTFIYAGPLPAWSTMNQIRQVMGDQPSE
                     EGAIDPKDVSLASYVEKKLGDFVRAMNVGSGKNLYPTLMRAVERPLIELALRETHGNQ
                     IKAARLLGLNRNTLRKKITEFQISVNQLKQSTAGTRKKTESDSV"
     gene            complement(7468..8955)
                     /locus_tag="VLA60_12285"
     CDS             complement(7468..8955)
                     /locus_tag="VLA60_12285"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HSF10185.1"
                     /translation="MSQSGQGPHPAIPAHQFGLFAAPLIAGLMFVLLPESLPDPARIL
                     STILSWVVVMWITEPIPLPITALLGCGLCVVAGLGDMQSVFSAFAHPIIFLFIGSFFI
                     SEALSVHGLDRRFGNWLLSRKCVGSNPIRIMMAMSLAVAGLSMWINNTAATAVMLPIA
                     LGVLRALRQSYPNMETFDTGFLLCLAYGAGIGGVATLVGTAPNLIGVGLLAQQAHFTI
                     SFDQWLLIGLPLASVMLLVMFTLMYWLHPPPSHSSFHEGSPVLKISAPLPWTRGEQYT
                     FGVFVLAVLLWLLPAILSIWFEGDHAVVQWVRAHLPKELVPIFASGLLFLLPLNFRQG
                     KFTLTWREAANIHWGTILLFGGGIAFGELMVETELAKAIGQGMVAMFGIESVWGLTGV
                     AILTALVLTELASNTAATSMIVPVLIAIAHTANFSPVPPVLGACMAASLAFVLPVSTP
                     PNAIVYGTGRIPILRMVQAGLLLDAIGGVLIWGALRIAGPLLGIV"
     gene            9149..9646
                     /gene="rfaE2"
                     /locus_tag="VLA60_12290"
     CDS             9149..9646
                     /gene="rfaE2"
                     /locus_tag="VLA60_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HSF10186.1"
                     /translation="MQSKIHTLDSLLQRISVHQQAGESIVFTNGCFDLLHIGHTRYLA
                     EAKQLGDRLVVGVNSDRSVRQLEKGKHRPIQTDAQRAEVIAALGCVDYVILFDEPDPL
                     NLIKAIQPNVLVKGGDWTSERIIGKDFVEERGGFVRTIPLVSDVSTTTIIERILTTHG
                     ISPSC"
     gene            9687..13166
                     /gene="mfd"
                     /locus_tag="VLA60_12295"
     CDS             9687..13166
                     /gene="mfd"
                     /locus_tag="VLA60_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HSF10187.1"
                     /translation="MNVPSVFHHFANQLRTVVPAIPEQTPPPCLLSTQQACLALAMTL
                     FTLPEKKTAGTDSQTHLIITPTQEQAEELYEDLEFFFSFIRRDHETLALFPPWATIPY
                     NPALPAHSVICQRVRSLHRLSRSESTVIVSSLPAILHKLLPPEVFAQACFSLKIGETC
                     EREVLLRSLIRLGYERGSLAEVPGEFSVRGGIVDIFSTAQDHPVRVEFLGDTVESIRT
                     FDPSTQESITHLKETWVLPTREFIPPDSGQPDSAIEHIQASIEWDLPRYYPKLVTLFE
                     YIQTPITVSFYRPVDLDAAAAQFWNALLETWEHLQPGQGGASGHADPDQLYEMWETIK
                     TYTSMCPTVWWDSVAPDTGTMPCVPVSLQAQSPSSVGVGIRGLPFKETLSKLDQLRAD
                     SEIFFVARSPGQVERLLSLLHDHGFPANKWQHPFPQPATDARAPFYCLEGDLSAGFLS
                     PDGHIAFVTEEELFGKGIRHRPHTKSPTAKFFSSLEDLKEGDLVVHLQHGIGRYLGLR
                     RLEVQGFTSDYLLLQFGGTDTLYVPLDRLNHIHRYRGAEQRSPRLDRLGGTAWGKTKA
                     KIKKGIEDMTEELVELYANREVARRSAYQEDTLLVHEFEAGFEYEETPDQLRAIEEIG
                     RDMESPKPMDRLVCGDVGYGKTEVAMRAAFKAIQGNRQVAVLVPTTLLAQQHFENFSI
                     RFAPFPVKIGILSRFQSANEIKATLKDLASGVIDIVIGTHRVVQKDVVFKQLGLVIID
                     EEQSFGVRHKERLKQLRTQVDVLTLSATPIPRTLQMAFSGVRDLSVIETAPPGRLAVE
                     TQVLRFDATVVREAIARELARGGQVYYVHNRVETMEQTGAWLQDLVPEARVLMAHGQM
                     NEQLLERVMLRFFHQEADVLLASAIIQSGLDIPSANTILVDRADLFGLSQLYQLRGRV
                     GRSGQQAFAYLFIPNEETLSTDAQKRMNAIQEFAELGSGFRIAAADLEIRGAGNLLGK
                     QQSGNIAAVGLDLYLQMVEQAVQQLKGQEVEEEWEPTLQLDVSAFIPEEYVEDASQRL
                     GLYKRLSGSDRLGDLALLYGETLDRFGSPPAAMERLFEVMQIKVLAKALQLEYVEVRG
                     GLMSLRFHERAKLPEPGIRALMDKWKDRLDCLSPRAYRLTLPEQDWASMYPRANTILQ
                     ELHQSMAKEEVRT"
     gene            13177..14088
                     /locus_tag="VLA60_12300"
     CDS             13177..14088
                     /locus_tag="VLA60_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HSF10188.1"
                     /translation="MRVCLKDGPFQTLTVWVVAILLFFPEVSLSLTDRIIAVVNKEVI
                     TSSDLEKELRDEYKRLNAKYHGEELNRRYFQKQREVLNHLIDDHLMIQEAQAKGLSVT
                     QDEIDEALRRTPLPPTQTEEEFGHQMLLKKLFDFEIRRNIVIEEEEVRRYYEANSTLF
                     LSPPRYRLQQILLAGREGYEREKAKNKAQTMYAAWTPNTTLEDFATRYFEQIHELGWV
                     QENELVAPLKEVVKELKPGQISAPIETLLGFHLIMVEEIQQPQPLPLEVVERDIRGLL
                     TKQRSEEAYRNWLADIKQKAFIDVRLK"
     gene            complement(14154..>15071)
                     /locus_tag="VLA60_12305"
     CDS             complement(14154..>15071)
                     /locus_tag="VLA60_12305"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HSF10189.1"
                     /translation="RVTLSEIAVRVAKERGIKVIAHGSTGKGNDQVRFENYITTLDAT
                     MKVIAPVREWSMGRDEQIDYAKQHDIPIKQTKEYLYSHDDNMWGSTNEGGDIEDPARI
                     PDLRKFLQVCIPPEQAPDEPEIVEVGFNKGIPCSINGEDLPLCQVIERANAVGARHGV
                     GIVHLTEDRLVGLKVRGVYEAPGAEILIKAHFNLEKLVNTRDLNELKKIIDEKWAYTC
                     YGAKWFDPSMDAIHAFQDSANQRVKGTVKVKLFKGKADVVAMTSPYSLFDANLATFNK
                     DASFNQNASAGFIEIYNLAQKTYRRLDQV"
BASE COUNT         3756 a         3976 c         3652 g         3687 t
ORIGIN      
        1 gtgcgaatgt tctggttgcc ggttccgcca tttttggcag caagaatatt cctgaaacca
       61 ttcggcagat gcgaactgca gctcagaccg tgattgtcta atcgggccca tgaattcccc
      121 aaataatacc gacagccttc atcctctcga aattcaggtc ctcagtgaac tggggaaata
      181 tcctcagcca ctttcagacc ttcaacttgc caaccatacg gagctagccc cttcacaagt
      241 tagtatggcg gtcgggtggc tgcttacgaa acaactggtg agcctgtcgt ccgaaaccac
      301 gacgacctat gtctcgctca cctcggtcgg cgcccaatac caccagccga actcttctcc
      361 acttgagtgg attcttcaga ccgtgaaaag tgccgctcat gacggctcat cccctaccgt
      421 gaaggacctt caaggcacgg ggaaattcca accctcagag ctaagccggg cgatcgggat
      481 tctcaagaaa gaaggcgtcc ttcagatggg tgcaggggga gtgctggaaa taaccagcaa
      541 ggatagtgac accatgaggg acttgcgaag cctgcttaat caggttcagg aaggtgcacg
      601 acccctggat tcgtttgcat ccgatcacca ggcattgctt cgccaatacg ccgtcaaacg
      661 cggcaatacg caagaaccct ttcgcatcga tgatcatacc gaacggacct acgtccttac
      721 agatgagggc cggaatcttc tccctcattt acgggaaggg gccgcccaag aaatttctca
      781 attaaccccg gaactcttaa aagacggatc ctggcgtcac gtatcctttc gcaaatactc
      841 catcaacctt cgccctcctc gcctagcggt cggcagacgg cattcctatc gggaatttct
      901 ggataccgtg aagtacaagc tcacgagcat ggggtttcag gaaatgcgtg gcgaacttat
      961 tgagacggaa ttttgggata tggacgcgct tttcatgcct caattccatc ctgctcgtgc
     1021 catccatgat gtgtattttg tcaagaatcc caagatggct aagaaaattc aagagccatt
     1081 tctcagccgg gtagggcagg tccaccggga tgggggaacc agcggatcga aagggtggaa
     1141 atacgagtat gaccctgagc gggcgaagcg cttagtcctc cgcagccaag gcaccgccgt
     1201 ttccgctcgg accttggcac aaaatccccc ggttccaggg aaattttttt ctattgcccg
     1261 gtgttttcgt tatgacaatg tcgatgccac gcatgccacg gacttttttc aggtggaagg
     1321 gattgtgctt ggctctgata tcaactttcg aacactcttg ggactcctgc aattgttcgc
     1381 cacggaaatg gcacaagcca aagagattcg atttttcccg gcttatttcc cctttaccga
     1441 gccctcggtg gaattgcatg tccgtcatcc caaacttgga tggatggaac ttgggggggc
     1501 cggccttttc cgctctgaag tcaccacacc tttaggggtg acggttccgg ttattgcatg
     1561 gggactcggg ttggatcgaa tggccatgat agcccttggg atacaagata ttcgcgattt
     1621 attcacttcc gatctagaag cggttcgcaa tatgcgccgt catttttagt tacgccccat
     1681 gccaaccatt tccattttcc aaaaagattt ctgtcgactt gtcggtcgtg atgcctccac
     1741 atccgacatt gaacaatgga tgccctatgt gaaaggggag gtaaaagacg tctcacagga
     1801 gacgggtgaa gtgcgcgtgg aacttcaaga taccaatcgg cctgatttat ggtgtgtgga
     1861 agggattgcc cgacaaattc gatcggtgct gaataaaggg atgccccctt attctttttt
     1921 ttcggaaaag aggggcgcca aacggcggat tcaagtggcc cagggcatgg aagtcgttcg
     1981 accgtttgtt gcgggttgcg tctcgttggg atatgccatg acgccggatg gcctcgacca
     2041 gtgcatccaa actcaggaga aattagccga cgcctttgga cgaaaacgtg aaaccgtgtc
     2101 cattggtctc tatcgtgcct cagccattgc ctttcccgtc acgtatggcc tcgtgaaacc
     2161 ggatgagatt cgatttacgc cattgggatt tgaggaaaaa atgaccccac aggaaatcct
     2221 caccgtccac cccaagggtc tggaatatgg atctattctc gctggatgcg aacggttgcc
     2281 gcttctttgg gattcagatg gacaggtgtt gtcctttcct cccattatca acagtcggga
     2341 gcttggtgaa gtgcagttag gagacacgga attactcgtg gaagtcaccg gaaccgattt
     2401 gagtatggtt gtcttggcct tgaacatttt tgcctgcaat ttagccgatc ggggagccac
     2461 catcgaatca ctggacatta actatcccta caaaacggaa tttggcgcta ccgttaaaac
     2521 cccgctgagc atgaatcaat cgcaaaggat ttccattgag gccattgaac aggccttggg
     2581 aatgccactc ggttcggagg ttattcaaga ggcccttgcc tcttatggat accaggtgaa
     2641 agcgaccagg cagtccgtat ccgtcacctt acccgccttt cgaaacgatt tcatgcatgt
     2701 gatggatgtc gcggaagatg tggccattac ccgaggctat gattcgtttt ctcccatcat
     2761 gccacaaacc ttcaccgttg gaagtctctc cttggaagaa caaaccagcg atcgcattcg
     2821 tgatcttctg gtcggatttg gatttcagga aattttttcc aatatcatgg cttcccatca
     2881 ggaattggtg gacagaatgc ggattgccga caccgaatat gcgcgactgg tagaagtgga
     2941 caacgtcatg tcccaaacct atgcctgtct gagaccttcc attttaccat gcctgcttcg
     3001 ggtggaagcc ctatcacctc gcgcgtttta tccccatctg ctttttgagg tgggagaaac
     3061 ggctcaactg gatttggagg ccaatgtggg ttctcgtacg accctgtccg ttgccgccat
     3121 tagcgccaat tcgggagcga atttttcaga aattcatagc tacctggatt tattgatgta
     3181 ttacatggcg tggccctacg aactggagcc agtcactcat ccctcatttt tagagggacg
     3241 agtcggccgt atccgttgtg aggggtatgc ggtaggatat atcggagagt ttcacccgga
     3301 agtactcgaa gcctggcaaa tcgatatgcc aacatccggg tttgaattcg agatcagaaa
     3361 gccggaagct taggcggcga cgggttgtcc gtctcccttg gtgagggtca caaggtgaga
     3421 aaagccttga ggatcgtgaa tcgccatttc tgaaagcatt tttcgattta ataacacatt
     3481 ggctttcttc agcgcattca tgaactgact ataggtgact ccttgcgcgc gaacagctgc
     3541 gctgatcctg gtgacccaga gctgccggaa attacgcttc ctctgttttc gtccggtgta
     3601 cgcataagct tgccccttat caactgattc ggttgcagtc cggaaaagct tactttttcc
     3661 tccatattgg ccactcgcca gtttgagccg ctttttccgg cgctgtcgag tttgtggccc
     3721 accttttacc cttggcatga cacgttctcc ttaatttttg aaatgttact tataggggag
     3781 taatcgagac agtgaagttc ggtcttcttt tctgacctct gctgaaccgc ttagtctgga
     3841 ttttactccg ggtgccttgg aagtgaggat atgacgcatt ccggccttcc gccgcatcca
     3901 cttgccagag ccggttcgtt taaatcgctt actggctcct gagtgatttt ttaatttcat
     3961 ttttgccatt tcttatattc ctttttaacg accggcaaat tgccggctct tcctcaactt
     4021 agccaattat tttggcgcga ccaccatgta aagattccgg ccctccattc taggaggact
     4081 ttcaatattt cctgcgccct ttaactcctc aataaccttc tgaatcgctt tgaacccaag
     4141 ctcttgattg gccatttctc gaccgcggaa cataaccgta actttggtct tattcccttc
     4201 ttccaggaag tcccgtattt gccggacttt cgtctccata tcgtgcttgt ctgtccgagg
     4261 ccgaaattta acttccttaa cctgggtgga tttctggtta agtcgcatgc gatgttcttt
     4321 tttgctctgt tcatacttaa atttcccata atccataata cggcagaccg gagggtccgc
     4381 ggtaggagcc acttccacca aatcatatcc aatttccctc gcctgccgaa tcgcctccga
     4441 tgtcttcagg attccaagct gttcaccttc agcaccaata acccggacct caggattttt
     4501 gatgaaatga ttaatacgtt gtttgggtgt caccggacga gtcattcgaa aagtaaagaa
     4561 cccttcctaa cagcctcggg catgtcttcc cgaattagcg tcaccaaatc aataataggc
     4621 atcgttccca aatttttccc gttcctttgt cgtaccgcaa ccatctttgc ttcagcttcc
     4681 cgatccccca cgacaatcat gaaaggaatc tttgcctttt cagcctcacg gattttaagg
     4741 ccaatctttt catttcgcaa gtctaagacg acgcgacagc cttcctgacg aagatttttt
     4801 tcaacctctt gtgcataccc ttgatgtttt tctgaaattg ggaggaccgt aacctgaaca
     4861 ggggccaacc aggccggaaa ggcaccccca aaatgctcca ggagaatccc gaaaaatcgt
     4921 tcaatcgatc ctaataaagc ccggtgaatc atgatgggtt gatgagtttt accatcatca
     4981 cccacgtagg tcaggccaaa cctctcggga ttattgaaat cgatctgaac cgttgagcat
     5041 tgccaggatc ggcctaaggc atcctgaatt ttgacatcga ttttaggccc ataaaagacc
     5101 ccttcccccg gatcttcctg atatgtatag ccgccgcttt tcagcgcagt ttccagcgcc
     5161 agcgtggctt gatcccattg ctcgatcgtc cccacagcct tttcgggccg agtagagaga
     5221 aacatcttga attccgtaaa tccaaacgtc cccagcatac tcgtaataaa tttcaggacc
     5281 ttttggacct cttcacccaa ctggtcagat cggcagaata aatgagcatc atcctgggta
     5341 aatccccgaa cccgcatcag gccatggagg actccggaac gttcgtaccg gtagaccgtt
     5401 cccaattccc cgtagcggat aggcagatca cgataactgc gcaaatgtga tttatacacc
     5461 atgatatgaa acgggcaatt catgggcttt aactgatatt cactggactc gaccggcatg
     5521 gaggcgaaca tattttcttt gtagaagtcc acatgcccgc tggttttcca taaatccaac
     5581 cgagcaacat gcggcgaata gaccaactca tactgattgg ctaaatgttg ttcccgccaa
     5641 aaattttcaa tcagcagacg gatttgtgcc cctttggggt gccataaaat aagaccgggt
     5701 ccggtttcgt cctgaatact aaataaatcg aggtcttttc cgagctttct atggtcacgg
     5761 cgtttgatct cctctaaatt atccaaatga gcctgcaagg cctctcttga ggggaaggac
     5821 gtgccataaa gccgctgcaa catggggttt cgttcatccc ctcgccaata ggcacctgca
     5881 ctattcagaa ttttaaaggc tttgacatag cccgtggccg ggagatgggg gccacgacag
     5941 agatcgacga agtccccttg ggtataagca gacaccggtt ctccatcagg aaacccttga
     6001 ataagctcta ccttatagaa ttcaccacga tttttaaaaa attctattgc ctcttcctta
     6061 gagaactcac gtcttgaaac cggaagattc cgtttgatga tttccaaagc cttggcttcg
     6121 attttttcta aatcttccgg cgtgaacggg cgctcaaagg caaagtcata aaagaagccc
     6181 tcttcgatgg ccggacctat cgttaattgt gctgaaggaa aacactcctt caccgcttgg
     6241 gccataatat gcgtgctact gtgccgataa atttctttgc cttctgggga atcaaacatc
     6301 aacggctgca aggtggaatc ggtctccagg gaagccagga ggtctatttc cacgtcattg
     6361 gccttcacgg caaacacctc aggtggcacg gctcccttca gcttctctag tgcggccttc
     6421 gccgagattc ctttgggaag agcctggcta ctttcgcctt gcagtgatac ctgaatagat
     6481 tccattagag taaaaacacc taataaaaaa aggatctaca ttccgtgatg aacgcaagaa
     6541 aaggcaccat tcctctcaat taaggaagct ggataccgtc aggctttttt ggagaaagat
     6601 aatggtaggc gcgggcggta ttgaaccgcc gacctctacc gtgtcaaggt agcgctctcc
     6661 ccctgagcta cgcgcctata tcttaccggc catatgctag actgccttgc cggtcttgtc
     6721 aagaaatggt tatttttcaa ggggaaatgg aaagactatc aaacagaatc cgattccgtc
     6781 ttttttcgtg ttccggcagt agattgcttt aactgattga cggagatttg aaattctgta
     6841 atctttttcc tgagagtatt gcgattgaga ccaagaagac gagccgcttt aatctgattg
     6901 ccatgcgttt cccgtaaggc cagttcaatc aggggccgtt caacagctct cattaaggtg
     6961 ggatagaggt ttttccctga gccgacattc attgctcgaa caaaatcccc caattttttt
     7021 tccacataac tggccaagct cacatctttc ggatcgatag ccccctcttc cgatggttga
     7081 tctcccataa cctgccggat ctgattcatg gtactccagg cgggcaaggg gcctgcataa
     7141 ataaatgttt ttgacaaatt cagttggcca ttcagtgccg acaaggcagg aaaaaatttc
     7201 ttcccggatt catccagaat cacgccggta aattgttggg agcgcatggc cgcttccagg
     7261 gaagagcagt catccaacac cgtaatggtg ttctggagat caggaccttg aacccgcgac
     7321 ttgagatcgg gatcctgggt taacagagca aaccgtatgt gagaagggtt caaagacacg
     7381 ttatttgggt caggtgaaat gccagcgagg attatgtcgg aacattattt aaaaatcaat
     7441 tgaggtctat ctcctaggtt aagaagctca aacaattccc aaaagtggac cagctatacg
     7501 taatgcgccc caaatcaaca ccccaccaat tgcatccaac aacaatccgg cttgcaccat
     7561 tcggagaatc ggaattctgc ccgttccata cacaatggca ttcggcggag tagaaaccgg
     7621 caacacgaat gccaggcttg cagccataca agctcccagt acgggaggga caggtgaaaa
     7681 attggccgtg tgggcaatgg cgatgagcac cggcacaatc atactggtgg ctgcggtatt
     7741 tgacgcgagc tccgtgagaa ccagagccgt aagaatagcc acacccgtta aaccccagac
     7801 ggactcgatg ccgaacatag ccaccatacc ctgaccaatc gctttcgcca attcggtttc
     7861 caccatcaac tccccaaagg cgatgcctcc cccgaataac aggatcgttc cccaatgaat
     7921 attggcggct tctctccatg ttaaggtgaa tttcccctgc cgaaagttca aaggcagaag
     7981 aaacaaaagc ccagaggcaa aaataggcac caattctttc ggcaaatggg cacgaaccca
     8041 ttgcacgacc gcatgatcac cttcaaacca tattgagaga atggccggga gcagccacaa
     8101 cagaacagcc aacacaaaca ccccaaacgt atattgctct ccccgagtcc agggaagggg
     8161 ggccgagatc ttgagaacag gagatccttc atgaaaagac gagtgagaag ggggaggatg
     8221 taaccaatac ataagcgtaa acatcacgag cagcatcaca ctcgccaacg gcaatccaat
     8281 cagtaaccat tggtcaaatg aaatcgtgaa atgggcttgc tgtgctaaca gccccactcc
     8341 gattaaattc ggcgcggtgc cgacgagagt ggccactccc ccaatgccgg cgccataagc
     8401 cagacacagc aaaaacccgg tatcaaaggt ctccatatta ggatagctct gccgaagcgc
     8461 ccgtaataca ccaagcgcaa taggcaacat gaccgccgtt gccgcagtat tgttaatcca
     8521 catagacagg ccggcaacag ccaaactcat agccatcata atacgaatag gattggaacc
     8581 cacgcactta cgtgacaaca gccagttgcc aaaccggcgg tccaacccat gaacagagag
     8641 ggcctccgaa ataaagaaac tcccgataaa caggaagatg atggggtggg caaaggcaga
     8701 aaacacggat tgcatgtctc ctaacccggc gacgacacac aatccacagc ccagcaaagc
     8761 ggtaatgggc agaggaatag gctccgtaat ccacatcacc actacccagc ttaaaattgt
     8821 tgacagaatt ctcgcggggt cgggcaggga ttcgggaagc agcacaaaca tcaacccggc
     8881 aatgagcggg gcggcaaaga gaccaaattg atgcgccgga atggcgggat ggggaccttg
     8941 accggattgg ctcatgcagc tcttggaatg gacgcactat ttaggacatc cggttggcct
     9001 gagaccgcga ccgcatatcc tgtttttcta tttgccgaag tggcttattg ggtaacctct
     9061 ctaggatttg gcgtatacag caggtatcat acagaaaacg ggatatgtga gatagggacc
     9121 ccaggaaagg attcaacgat ttattcttat gcaatccaaa attcatacct tggacagtct
     9181 cttacaacga atttccgttc atcagcaggc gggagaatcc attgtcttta cgaatgggtg
     9241 ttttgatttg ctgcatatcg gacatacgcg atatctggca gaagccaagc agttgggaga
     9301 tcgattagtg gtcggagtca acagcgatcg ttcagtccgt caactggaga aggggaagca
     9361 tcgaccaatc caaaccgatg cccaacgggc ggaggtcatt gccgcattag gttgcgtaga
     9421 ttacgtcata ctttttgacg agccggatcc cttgaatctc attaaggcta tccaacccaa
     9481 cgttcttgtg aaaggaggag attggacctc cgaacgaatt atcggaaaag attttgttga
     9541 ggaacgggga ggatttgtgc gaacgattcc acttgtctcc gatgtttcga ccacaacgat
     9601 tattgaacga attctcacca ctcatggaat aagcccatcc tgctagtccg cacgtcgaga
     9661 acctttttcg tggggtcttt cagattatga acgttccttc cgtcttccat cattttgcaa
     9721 accagctgcg cacggtcgtt ccagcgatcc cggaacaaac gcccccgccc tgtctgctga
     9781 gtacgcaaca ggcctgcctc gccctggcca tgaccctgtt cacccttccg gagaaaaaga
     9841 ccgccggcac tgacagccaa acacacctca tcatcacgcc cacacaagaa caagctgaag
     9901 aattgtatga agatttggaa tttttcttct cgttcatcag gcgtgatcac gagacactgg
     9961 cattattccc tccatgggcc accattccct ataaccctgc actgccggcc cacagcgtta
    10021 tttgtcagcg cgttcggtcc ctccaccggt tgtctcggag tgaaagcacc gtcatcgtga
    10081 gctcacttcc ggccatcttg cataaactgc ttccgccgga ggtcttcgct caagcatgtt
    10141 tttccctcaa gataggcgag acgtgcgagc gtgaggtgct tcttcgttca ttaatccgac
    10201 tagggtatga acgtggatcg ttagctgaag tccccggtga gttcagcgta cgtggcggta
    10261 ttgtggatat tttctccacg gctcaggacc atcccgtccg tgttgaattt cttggtgata
    10321 cggtcgaatc catccggacg tttgatccct ccactcaaga atccatcacc catctcaagg
    10381 aaacctgggt cttacccact cgggagttta ttcctccaga ttccggacaa ccggatagcg
    10441 ctatcgaaca cattcaggca agcattgaat gggatcttcc ccggtattat cccaaacttg
    10501 tcacactgtt tgagtacatt cagacgccga tcaccgtctc tttctaccgc ccggttgatc
    10561 tggatgctgc ggctgcgcaa ttttggaatg cgttgttaga aacctgggaa cacctccagc
    10621 ctggtcaggg gggcgcctcc ggccacgctg accccgatca actctatgaa atgtgggaaa
    10681 ccattaaaac ctataccagc atgtgtccaa cggtatggtg ggatagcgtg gctcctgata
    10741 ccggcaccat gccctgtgtg cctgtttcct tacaggcgca gtctcccagc agtgtcggtg
    10801 taggcattcg cgggcttccc tttaaggaaa ccctttccaa actggaccaa ttgcgggcgg
    10861 attccgagat cttttttgtc gctcgcagtc cgggacaggt ggaacgcctg ttgtccctct
    10921 tgcatgatca cggcttcccc gccaacaagt ggcagcaccc cttccctcaa cccgccaccg
    10981 atgcccgcgc acccttttac tgtcttgaag gagatctatc ggcagggttc ctctcacctg
    11041 acgggcacat cgcgttcgtc accgaggaag aattgtttgg gaagggcatc cggcatcgtc
    11101 cccacacaaa atcccccacc gcaaaatttt tctcgtcgct ggaggatctg aaagaaggcg
    11161 atctcgtcgt ccatcttcaa catggaattg ggcggtatct gggtttgcgt cggttggaag
    11221 tgcagggttt taccagtgat tatttgctcc tgcaattcgg tggaaccgat accctgtatg
    11281 tcccgctgga ccgcctgaac cacatccacc gctatcgggg agcggaacaa cgctccccgc
    11341 gtctggaccg cctgggaggc acggcttggg gcaaaaccaa ggcgaaaatc aaaaaaggca
    11401 tcgaggatat gaccgaagaa ctcgttgaac tctatgccaa ccgggaagtc gccagacgga
    11461 gcgcatacca ggaggacacg ctcttggtcc atgaatttga agcgggcttt gaatatgaag
    11521 aaacgcctga ccagcttcga gccattgaag aaataggacg ggatatggaa tccccgaaac
    11581 ccatggaccg cctggtctgt ggagatgtgg gatacggcaa aacggaggtc gccatgcgtg
    11641 ccgcctttaa ggcgatccag ggcaacaggc aggtggccgt cctggttccc acgaccttgc
    11701 tggctcaaca acattttgaa aatttttcaa tacggtttgc cccattcccg gtcaaaatcg
    11761 gcattttgtc acgctttcaa tcagccaatg agatcaaagc tacactgaaa gacttggcca
    11821 gcggggtgat cgacattgtg atcgggacgc accgggtcgt gcagaaagat gtcgtcttta
    11881 aacaattggg cttggtgatt attgatgaag aacaatcgtt tggtgtccgc cataaagaac
    11941 ggctaaagca actccggacc caggtcgatg tgctcacgct gtccgccacc cctatccccc
    12001 gaactctgca gatggccttt tcaggagtca gagacctatc cgtcattgag accgcgcctc
    12061 cgggtcgttt ggcggtggaa acccaggtcc tccggtttga tgcgacagtc gtacgggagg
    12121 ccattgcccg ggaactcgct cgtggagggc aggtgtacta cgtgcacaat cgagtggaaa
    12181 ccatggagca gacgggtgcc tggttacaag atttggttcc ggaagcacgg gtattgatgg
    12241 cccatggcca aatgaacgaa caattattgg aacgggtgat gttacgattt tttcaccagg
    12301 aagccgatgt gctgctggcc tcggccatta tccaatcagg tttggatatt ccaagcgcga
    12361 acaccatcct ggttgatcgg gcggatttat tcgggctctc ccagctgtat caacttcgcg
    12421 gacgggtggg gcgaagcggg caacaagcct ttgcctacct cttcattccg aacgaggaaa
    12481 ccctgagcac cgacgcacaa aaacgcatga acgccattca agaatttgcg gaacttggct
    12541 ccgggtttcg aatcgcagcc gctgaccttg aaatccgggg agcgggaaat ttactcggga
    12601 aacaacagtc cggcaatatc gccgctgtcg ggttggacct gtacttacaa atggtggagc
    12661 aggctgttca gcagcttaaa ggccaggaag tggaagaaga atgggaaccc acccttcaac
    12721 tagatgtttc ggccttcatt cccgaggaat acgtggaaga cgcctcccag cgcctgggac
    12781 tctacaaacg gctatccggg agtgaccggt taggggattt ggccttatta tatggggaga
    12841 cgcttgaccg gtttggatcc ccccctgccg ccatggaacg actatttgaa gtcatgcaaa
    12901 tcaaagtgtt ggccaaggcc ctacaactcg agtatgtcga agtccggggc ggactgatga
    12961 gcctgcgttt tcacgaacgg gccaagctcc cggaaccggg aatccgtgct ttgatggaca
    13021 aatggaaaga tcgcctggac tgtctttctc ctcgtgccta tcgtttgacc ctgccggagc
    13081 aggattgggc atccatgtac cccagggcta acacgatttt acaggaactc catcagtcga
    13141 tggccaaaga ggaagtgaga acataacgtg aagggcatgc gggtatgcct gaaagacggg
    13201 ccttttcaga cgctcaccgt ctgggtggtg gcgatccttc tcttttttcc cgaggttagc
    13261 ttgagtctca ccgaccggat tattgccgtg gtgaataaag aggtgattac ctcgtctgat
    13321 cttgaaaagg aattgcgcga tgagtataag cgcctgaacg ccaaatatca cggagaggaa
    13381 ctgaatcggc ggtatttcca gaaacaacga gaagtcctga accacttgat tgatgatcac
    13441 ctcatgattc aagaagctca agccaagggg ctctctgtca ctcaggacga gatcgatgaa
    13501 gccttacggc gaactccgct tccacccact caaacggaag aggaattcgg gcatcaaatg
    13561 ctcctcaaaa aactctttga cttcgagatt cgccggaaca ttgtcataga ggaagaagaa
    13621 gtccggcggt attatgaggc caactccacg ctttttctct caccgccccg gtaccgcctc
    13681 cagcaaattc tgttagccgg gcgggaaggc tatgaacggg agaaagccaa gaacaaggcc
    13741 caaaccatgt atgcggcatg gacaccgaat acgacgctcg aggatttcgc caccaggtat
    13801 ttcgagcaaa ttcatgaatt aggctgggta caggaaaacg aattagtggc ccctctgaag
    13861 gaggtggtca aggaactgaa accgggacag atctcggccc ctattgaaac cctcttagga
    13921 tttcacctca tcatggtaga agaaattcaa caaccccaac cgctcccgct tgaagtcgtg
    13981 gaacgggata tccgggggtt acttactaaa caacgatccg aggaagcgta tcgaaattgg
    14041 cttgccgata tcaaacaaaa agctttcatc gatgtgaggc ttaaatagag gattcatttc
    14101 accgctgatc gctcaagacc ttctcccatc acgaatccct gtcatactcc atcctatacc
    14161 tggtctaagc gccgataggt cttttgggcc agattgtaaa tctcaataaa acctgcagag
    14221 gcattctggt tgaaactcgc atccttatta aaagtggcca aattggcatc gaataaggaa
    14281 tagggggagg tcatcgcaac cacatcagct tttcctttaa acagctttac ttttaccgtt
    14341 cccttcacac gctgattggc ggaatcctga aaggcatgga tggcatccat tgatggatca
    14401 aaccatttag cgccataaca ggtgtacgcc cacttttcat caatgatttt tttcagttcg
    14461 tttaaatcac gggtattgac gagtttttcc agattaaaat gcgctttgat cagaatttcc
    14521 gctcccggag cttcatacac accacgaacc ttcaatccga ctaagcggtc ctccgtcaaa
    14581 tgcacaatgc ctaccccatg ccttgcccct accgcattgg cacgttcaat gacctgacaa
    14641 aggggcagat cctcaccatt gattgaacaa gggattcctt tgttgaaccc gacttcgaca
    14701 atctccggtt catccggtgc ctgttccggt ggaatgcaca cctgcagaaa ttttcgtaaa
    14761 tcaggaatgc gggccggatc ctcaatatcc cctccctcat tagtcgagcc ccacatattg
    14821 tcatcatgcg aatacaggta ctctttagtt tgtttaatgg ggatatcgtg ttgtttggca
    14881 taatcaatct gttcatcccg tcccatactc cattcacgta ccggcgcgat cactttcatc
    14941 gtcgcatcca aagtcgtgat gtaattttca aagcgaacct gatcatttcc ttttcccgtg
    15001 gagccgtgag caatgacctt gattccccgt tccttggcca cacgaacggc aatttccgaa
    15061 agcgtcaccc g
//
LOCUS       DATIAL010000396         4054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1362605, whole genome shotgun sequence.
ACCESSION   DATIAL010000396 DATIAL010000000
VERSION     DATIAL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362605"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VLA60_12310"
     CDS             complement(<1..263)
                     /locus_tag="VLA60_12310"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HSF10190.1"
                     /translation="MRIGQGYDIHPLREGRPLILGGVVIPHSQGLDGHSDADALTHAV
                     CDAILGAMGEGDLGTRYPSSNPEYKNLSSLVMLRSVAQTLRDKG"
     gene            complement(320..1024)
                     /gene="ispD"
                     /locus_tag="VLA60_12315"
     CDS             complement(320..1024)
                     /gene="ispD"
                     /locus_tag="VLA60_12315"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HSF10191.1"
                     /translation="MFNSVVAVVPAAGLGTRMGGNTPKQYLTLGNLPLLVYSLQVFQQ
                     LEEICEVILSVPASDREYCWREIVKPFGLEKVAKVVAGGARRQDSVRNGLAAISDRPD
                     GVLVHDGVRPFIDQGMVRNVIDCAGKTGAAVVAMPIHDTVKRVDASQIIQETLKREEL
                     WQIQTPQVFRYDWLVEAHQQAQQHQWEVTDDAALIERMGYPVSVVEGSCFNIKVTKPE
                     DLVFGKAILETIGSRR"
     gene            complement(1017..2042)
                     /locus_tag="VLA60_12320"
     CDS             complement(1017..2042)
                     /locus_tag="VLA60_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HSF10192.1"
                     /translation="MVLRMIFLGVGVVLGMALAWGATDGQIGVLLMGGGIGAGVGAII
                     LAVEHRLKATPLPFVVCGGGGLVVGLLVAGLIASVTGLVARSPNTVFNILASLVIFLG
                     IPYWGLIMGIRFATEGWAPSAIPTGDTESVRIKKLLDTSVIIDGRIADLCETGFIEGT
                     LVVPHFILQELQHISDSSDGLKRARGRRGLDILNVLQKVSNIKVDLVEDDFPHVKEVD
                     TKLIELAKQMDAKVLTNDFNLNKVAGIQGVRVLNINDLCNALKPVVLPGETIRVFVLK
                     EGKEAGQGVAYLDDGTMVVVDHAKRWIGKNADVIVTSVLQTSAGRMIFTRLKEETEHE
                     ELSFSRV"
     gene            complement(2059..3066)
                     /gene="fbp"
                     /locus_tag="VLA60_12325"
     CDS             complement(2059..3066)
                     /gene="fbp"
                     /locus_tag="VLA60_12325"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HSF10193.1"
                     /translation="MTPRFSLSTHTFREQAPIPGTSGEFSRIIMQIALGGKLIAQDLR
                     KAGLSQFLGSTGLINVQGEEVQKLDERANQIFLDVFEHMELVSTLVSEEMEKPYLIKE
                     ADGQGRYAVFLDPLDGSSNIDVNASLGSIFSIHRLSIEGFPTSEDALRKKGCDQVAAG
                     YILYGSSVLLVYTCGHGVHQFTLDQEAGEFFLSAKNIQIPKHGKIYSVNEGNYQKWST
                     GTQQFLHYLHEVDVQTGRPYTSRYSGCFVADVHRVLCKGGLYMYPGEQKNPEGKLRLM
                     YEAAPLSFLVEQAGGMGSTGVEPVNQLIPKALHQRVPLYIGSQDDVTKAEAFLRSESP
                     I"
     gene            complement(3326..>4054)
                     /locus_tag="VLA60_12330"
     CDS             complement(3326..>4054)
                     /locus_tag="VLA60_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HSF10194.1"
                     /translation="ETGADVGIGYDGDADRIGVIDEQGQIMWGDRLLLLFARDVLNEH
                     PGCSIISEVKASQGLYDDIQKQGGRGIMWKTGHSIIKAKMKEEKALLAGEMSGHLFFA
                     DRYYGYDDAIYASCRLIEILVKRKMPLSALLADIPQTVVTPEIRVDCPDDQKFLLVEI
                     VKNRLQHEANTQNSKTSVLPIRDIITIDGIRVRFDEGWGLIRASNTQPALVLRFEASS
                     HTHLTQIQAYLEGQLSEATVDLTT"
BASE COUNT         1096 a         1116 c          855 g          987 t
ORIGIN      
        1 cctttatcac gaagggtttg agcgacactt cgtagcataa ccaaactcga gagatttttg
       61 tattctggat tactgcttgg gtaccgtgtt ccgagatctc cttctcccat ggctcctaga
      121 atggcgtcgc aaacggcatg ggtcaaggca tcagcatcgg aatggccatc caacccttga
      181 gaatgtggga tgaccacgcc acccagaatc aggggacggc cttcccggag gggatgaatg
      241 tcataacctt gaccaattct catatcacat caaagacgag cgaaaaaaaa tccatctgaa
      301 ggaaggctgg cctgaaattt caccttcggc ttccgatcgt ttccaaaatc gctttcccaa
      361 acaccagatc ctccggcttg gtcaccttta tattaaaaca acttccctcc acaacggaaa
      421 cggggtaacc catccgttcg atgagggccg catcatcggt gacttcccac tgatgttgtt
      481 gcgcctgttg gtgtgcctca accagccagt catatcggaa aacctgcggt gtctgaattt
      541 gccagagttc ttccctcttc agcgtctctt gaatgatctg agatgcatca acccgcttca
      601 ccgtatcatg gatcggcatt gccactaccg ccgcacctgt ttttccggca caatcgatca
      661 cattcctgac catgccctgg tcaataaagg gccgaacacc atcatgaacc aaaaccccgt
      721 caggtcgatc ggaaatggcc gcaagtccgt ttctgacaga atcctgtcgc cgcgctcctc
      781 ccgccaccac tttggcgact ttctccagac caaaaggttt cacgatttca cgccagcaat
      841 actcccgatc cgatgccgga acggagagaa tgacttcgca gatctcctcg agttgttgaa
      901 aaacctggag tgagtacact aaaagaggca gattcccaag agtaagatat tgcttggggg
      961 tatttcctcc catgcgggta ccaaggcccg cggcaggaac caccgccacc acagaattaa
     1021 acacgcgaga agcttaactc ctcgtgttca gtttcttctt tcaggcgggt aaaaatcatt
     1081 cggccggcac tcgtttgtag aacactcgtc acaatcacat ccgcattttt gccgatccat
     1141 cgcttggcat ggtcaaccac caccatggtg ccatcatcca aataggccac accttgcccg
     1201 gcctctttcc cttctttcaa aacaaaaacc cggatcgtct caccgggaag aaccaccggt
     1261 ttcagcgcat tacacaagtc attaatattc agcaccctga cgccttggat cccggcaacc
     1321 ttattgaggt taaaatcatt ggtcagcacc tttgcatcca tttgcttcgc aagttcaatg
     1381 agctttgtat ccacctcttt cacatgagga aaatcatctt ctaccaggtc taccttaata
     1441 ttgctgactt tttgaaggac attcaaaata tccaaccctc gtctcccacg tgcccgcttt
     1501 aacccatcag aggaatccga aatatgctgt aactcctgga gaataaaatg tggaacaaca
     1561 agggttccct caataaagcc cgtttcgcac aaatctgcaa tccggccatc aatgatgacg
     1621 ctggtatcaa gcagtttttt gatgcgaacc gactccgtat cacctgttgg aatcgctgat
     1681 ggggcccatc cttctgtggc aaatcgtatt cccatgatta atccccaata gggtatcccc
     1741 aaaaagatca ctaagctggc caaaatgtta aagacagtat taggagagcg tgcgactaat
     1801 cccgtcaccg aggcaatcaa acccgccaca aggagaccaa caactaatcc tccccctcca
     1861 cacacaacga agggtaaagg cgtggccttc aatcggtgtt ccacagccag aataattgca
     1921 ccaaccccgg ccccgattcc cccgcccatg agcagaacac ctatttggcc gtcggttgct
     1981 ccccatgcca aggccatacc taagaccacc ccaacgccta gaaagatcat tcgcaacacc
     2041 ataggagatt ctccttccct agatcggtga ctcagaccgc aggaacgctt cagctttggt
     2101 cacatcatcc tgactgccaa tatataaagg gactctctgg tgaagggctt tcgggattag
     2161 ttggttgacg ggttcaacac ccgtacttcc cataccaccg gcttgttcaa ccaaaaatga
     2221 taagggtgcg gcttcataca tgagcctcaa ttttccctca gggttcttct gctctcccgg
     2281 atacatatag agaccaccct tgcacaacac tcgatgcaca tcagccacaa aacacccgga
     2341 ataacgactg gtgtatggtc tgcctgtctg aacatcgact tcgtgaagat agtgaagaaa
     2401 ttgctgagtg ccggtagacc atttttggta attaccttca ttcacactat atatttttcc
     2461 atgcttgggg atttgaatat tcttggctga gaggaaaaac tcaccggcct cctggtccaa
     2521 cgtgaattgg tgcacaccat ggccgcaggt atacactaaa agcacactcg atccatacaa
     2581 gatatatccg gccgcaacct gatcgcaccc tttttttcgc aaggcatctt cagaagtagg
     2641 gaaaccctca atagaaagtc gatgaatgga aaaaatggac cctaaagacg cgttgacatc
     2701 aatgttagat gagccatcca aaggatccaa aaagaccgca taccgtccct gaccatcggc
     2761 ttctttaatg aggtaaggct tttccatttc ctctgaaacc agcgtactca ccagttccat
     2821 gtgctcaaac acatccaaaa atatctgatt ggcccgttcg tccaactttt gaacctcttc
     2881 gccttgcaca ttaattaacc ccgtagaacc caggaattga ctcaatccgg cttttcgaag
     2941 atcctgggca atgagtttcc cacccaaagc aatttgcatg atgatgcggg aaaattcacc
     3001 ggaggttccc ggaataggcg cttgctcacg aaaggtatgt gtcgataagg aaaaacgcgg
     3061 agtcatcaat cctaatccat agactatact agaggatttt cattatatgc ttttccgcat
     3121 aaaggagcaa cgcacgttcg agaggaattc gcgttcgttc gggaatgaat attctcacgg
     3181 atgaatccgg ctggcttatt caatgccgag ccttcatggt gatggatatc acagtgttcc
     3241 tgatctgatc gacgatgcct tcaccagaag cggttgagaa tgtctacagg aacgatctaa
     3301 aaaagtaaaa taacccgggg agagcttagg tggtgaggtc taccgtggct tcagaaagct
     3361 gtccttccag ataggcctga atttgagtga gatgagtgtg ggaagaggct tcaaagcgaa
     3421 ggactaatgc tggttgcgtg tttgatgccc gaatcagacc ccacccttcg tcaaaacgaa
     3481 cacgaatgcc atcaatggtg atgatgtcac gaatgggcaa aactgatgtc ttcgaatttt
     3541 gcgtgttagc ctcgtgctgc aaacggtttt tcacgatttc gactaataaa aatttttgat
     3601 catccgggca atctactcga atttcaggag tcacaacggt ttgaggaatg tcggccaaca
     3661 gcgccgacaa gggcattttc cgcttcacaa gaatttcgat aagccgacaa gaagcataaa
     3721 tagcatcgtc atatccataa taccgatcgg caaaaaacag gtgacccgac atctccccgg
     3781 caagcaaagc cttttcctct ttcattttgg ctttgatgat ggaatgtccc gtcttccaca
     3841 tgataccccg tccaccctgc ttttgaatat cgtcatacaa cccctgagag gctttcacct
     3901 ctgaaataat ggaacaccct ggatgctcat tcagcacatc ccgggcaaaa aggagtaata
     3961 accggtcacc ccacataatc tggccctgtt catcaatgac gccaattctg tcagcatcac
     4021 catcgtaccc gattcccaca tcggctccgg tttc
//
LOCUS       DATIAL010000397         4807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1578796, whole genome shotgun sequence.
ACCESSION   DATIAL010000397 DATIAL010000000
VERSION     DATIAL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578796"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VLA60_12335"
     CDS             complement(<1..203)
                     /locus_tag="VLA60_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10195.1"
                     /translation="MSIKKLSSAQFRLRLLNLMTQKHHWAWPRIVGPTISKAQLKIHY
                     QQEYAVYVRDFPVFLARIHGQNPP"
     gene            905..1309
                     /locus_tag="VLA60_12340"
     CDS             905..1309
                     /locus_tag="VLA60_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10196.1"
                     /translation="MATCLLLPNKVMGNRINALKVSTVNTRRDQPMMYVDSRYISLAR
                     KSELLHHEHAMNQLRRQVAQLSKAVAWELEQHHSHLKELQQVEDTLTGKSQPVHATQP
                     NAESAPPTTGRWTKQRKHNPRSFQKPVAELVG"
     gene            2313..>4807
                     /locus_tag="VLA60_12345"
     CDS             2313..>4807
                     /locus_tag="VLA60_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HSF10197.1"
                     /translation="MDERRGYTRLPDMLSKLQKKGFVIQAQAHPSTELTTISNLGIPP
                     IYRVKAQGGLHIVDSHGQDLFQHADPKRVFPNFEAIPPLLVQTLLFIENRELLDPCCP
                     YANPAVEWDRLGQAFLTQGIQLVKNDQNVPGGSTLATQLEKFRHSPGGQTSSLMEKAK
                     QVTAASLRIYHDDVRTQEAQKHVVLDYMNSFPLGAYPGAGEIHGFGDGLSAWFHMDLN
                     RFDQILAGADPTSLEKMALAAATYKKGVSLLLALRRPSHYLTHIDALNEKTNIYLRLL
                     TSAGIISPALRDLALIYPLVFAPASSTNSSNTSFQDQKAPNFIRAHLQNLLGLPSLYA
                     LDHLDATVETTLDRDLQFRATTLLQHLADPAFVKDSQLTQPHLLAQGNPADIIYSLTL
                     YERTPYGNLLRVQTDTLNQPLDMNQGSKMDLGSTAKLRTLVTYLEAIERLHHAYAGQS
                     PQKLLELNQQSLDPLSRWALTYLQTSSDQSLSGILKAALNRQYSASPTERFWTGGGLH
                     TFANFNRADNGKLFTVREAFHHSVNLVFIRLMRDLVQYHTLAIPGSTAMVLKDPLNPI
                     RRQYLQKFAQQEGRIFLNRFYDKYKGLSPEEAWQLVLSQTRLTPLRLAVLLRSIEPEK
                     DVRAFTTSLRQTFPTIKLSPEQADRLFTQTDPRVLSLADRGYVARIHPLELWTVAFLR
                     QHPDSGKSELANASEQELLEVYAWLFKTHRKAAQDSRIRLILEQEAFMEIHKAWKRVG
                     YPFATLVPSLATAIGSSADRPAALTELMGILVNEGRKTPTVTIRQLHFAEGTPFETLV
                     APQEPNQEQILNPLVAQILRQELRSEERRVGKE"
BASE COUNT         1305 a         1351 c          968 g         1183 t
ORIGIN      
        1 ggaggatttt gcccgtgaat acgtgccagg aaaacgggga agtcccggac atacacggcg
       61 tactcttgtt gataatgaat tttgagttgc gctttggaga tggttggccc aacgattcga
      121 ggccaggccc aatgatgctt ctgagtcatg agattcagca atctcagacg aaattgcgct
      181 gaagatagtt tcttgatgga catcctgtat ttcttgttaa cgattcggtg aatcacccat
      241 ccatcaatgc tcagaccaaa tccaacttac taaccattcg gatttcaatc gattttctgg
      301 agtctttaat tttttgcggg attgatcacc acaaaatcag ccattgaaga atgatttctg
      361 gctcatatat agatcttgtc caaacattca gaatagcagg ataagttaaa taggcaactt
      421 aaagaagaaa ttaaagaatc ccctcgaaag cacgagcttc tctaccataa caaaaacaaa
      481 taattacacc tcaaaccgta tcatattcag tgtcattacc ttcatccttt ttctttctgg
      541 accaagggaa tcaaagactt gacataataa aaatagcata aaaagaaaca agatcggaaa
      601 aattttgcca ggcaactatc aatgtgaggc attctacaaa atgtgccaat ctgcatcaga
      661 atggaaaggc ccgtaaatat aatattcgtc gaaaattttt tttcacgttc ctggcgcttc
      721 atctcagatg cactcaagcc tatcttgatg cttaacaatt tatgaatcgt tttgtgctga
      781 tggtaaaaaa cagtgaccgt actcagccat ttcaggaatc gagcatacac cagatggata
      841 tttagtgata aaaattcatt gaaacatcaa gaccttagat gtctaatcct gaggaaaacg
      901 gaatgtggca acatgtttgc ttttgcccaa taaagtaatg ggcaatcgga ttaatgcatt
      961 gaaagtatca accgtcaaca cacggaggga tcaaccaatg atgtatgtgg atagccggta
     1021 cataagtctg gctcgcaaaa gcgaattatt acaccatgag cacgccatga accaattacg
     1081 tagacaggtg gcacaactct caaaagcggt tgcgtgggaa ttggaacaac accactctca
     1141 tctcaaggaa ctccaacagg tggaagatac tcttactggt aaaagccagc ccgtacacgc
     1201 tactcagccg aacgcggagt cagctcctcc tactaccggc agatggacta aacagcgaaa
     1261 acacaatccc cggtcctttc aaaaaccagt agccgaactc gtcggatagc ttttcccagt
     1321 caaagttcat cgcttgaccc acggaatttc cgtgggtcat ctcttttccc tcctcaggtt
     1381 ccttccaaag cgccataggc aaaatcttct gccaatctcc tcacatagcc ccccaaacaa
     1441 ggtattgccc actttctccc ccctgacgac atgatgaggt cgctcgaaac ccctatgcgc
     1501 atattttcct ataagcaaga gacccggaag cctccaactc aatcgggagg actcccgttg
     1561 cacgaacgca gattgggtat tcacctatcg ccctgttttg cactaactac cggtgacttc
     1621 ctctttccca aagcactgtc tccctttttc tcaacttaca gccttttgtc cacaattcag
     1681 catttaacct ggacaacttg tccaggattg aaagcacatc caccaacaac tgaaaacatc
     1741 ctggcgcaca atttatcaag ttcacccagt tgtaaattta ctaacttggc tcgccaattc
     1801 actttttcaa tcaaaggaaa ctgctctgtt atcgcacttg gccataccgg cagcatatga
     1861 aacccaaaaa accttcgtac attttttgca caattgtatt tcgaaacact tggctaggat
     1921 ggtttacgtt ccgatatcac aggtccatgc tcttttatat acagaccggg ccattccgta
     1981 aaccagcgac ttttctgata tctcttctct tatcaagaaa ggccctgacc tttcctcctg
     2041 ccgacccttg ggtcggcgct gaaaccacac ccggtaattc ccagcaaatc gagaaatggc
     2101 gaaaccttgg tcgcctcctt caaagccttc gcccccccta ccaaaccact catattgtct
     2161 tcctggccac attgactttt ttgggctggg cgatatttga actgaacact tcctggcttc
     2221 aatcccgtct atttcatgca atctccgctc aactaacatc ccaagtaaaa ccggggccct
     2281 ccccactcac agtgtttcca tctgcaggcc ccttggacga acggcgcggg tatacccgac
     2341 tccccgatat gctctccaag ctccaaaaga aaggattcgt cattcaggcg caagcccatc
     2401 catcgactga actcacaacc atctccaatc tgggtatccc tcccatctac cgagtaaaag
     2461 cccagggcgg gttacacatt gtggatagtc atggtcagga cctgtttcaa catgcagacc
     2521 cgaagcgagt ctttcccaat tttgaggcaa ttccccctct gttggttcaa accctccttt
     2581 ttattgaaaa tcgagaatta cttgatccct gctgtcccta tgccaatccg gccgtggaat
     2641 gggaccggtt agggcaggca ttcctcacgc aaggcatcca attggtgaaa aacgaccaga
     2701 acgttcccgg gggaagcact ttggccacgc aattggagaa attccggcat tctcccggag
     2761 gccaaacctc ctccctgatg gaaaaagcca aacaggtaac ggccgccagt ctcaggattt
     2821 atcatgacga cgtacgcaca caagaagctc agaaacatgt ggtcctggat tatatgaatt
     2881 cattcccgct cggcgcgtat cctggtgccg gggagatcca tggattcggt gatggactat
     2941 cggcctggtt tcacatggac ctcaaccggt ttgaccagat tctggctggt gctgacccga
     3001 catctttgga aaaaatggcc ttggcagcag ccacttacaa aaaaggagtc agtctccttc
     3061 tagctctcag gcgaccctcc cattacctca cacacattga tgccctgaac gagaaaacca
     3121 atatctatct tcgcctcctg acaagcgccg gcattatttc ccctgctttg cgagacctcg
     3181 cacttattta tcccttggtc tttgcgccag catcatccac gaattcaagc aacacctcct
     3241 ttcaggatca aaaagcacca aatttcatac gcgcccattt acaaaattta ttagggcttc
     3301 caagtcttta tgccctggac catctggacg ccaccgtgga aaccaccctg gaccgcgatc
     3361 tccaattccg agcgaccacg ctcctacaac acctggcaga ccccgccttt gtgaaagact
     3421 cccaactgac tcaacctcat ctgcttgctc aagggaatcc ggccgatatc atttatagcc
     3481 tgactctgta tgagcgaact ccttacggca atctccttcg agttcaaacc gacaccctca
     3541 atcaaccatt ggatatgaat cagggctcaa aaatggattt gggttccact gctaaacttc
     3601 gtacattggt cacttatctg gaagccattg aacgcctcca tcacgcgtat gcggggcaat
     3661 caccgcaaaa actcttggag ctcaaccagc aatccctgga cccacttagt cgctgggcat
     3721 taacctacct tcaaacttct tcagaccaat ccctctctgg gatattaaaa gccgccctca
     3781 atcgacagta ttcagcgagt ccaaccgaac ggttttggac tggaggcggg cttcacacct
     3841 ttgccaattt caacagagcc gacaacggga aactgtttac tgtccgggaa gcttttcatc
     3901 actccgtgaa tttagtcttt atccgcttaa tgcgggatct cgtccagtat cacactctcg
     3961 ccatacccgg atcgaccgcg atggtcctca aagaccctct caatcccata cgccggcaat
     4021 atttacaaaa attcgcccaa caggaaggtc gaatctttct caatcgtttc tatgacaaat
     4081 acaaaggact ttcgccggaa gaagcctggc agctggttct ttcccagact cgactcacac
     4141 cgctccgact ggctgtcctc ctgcgctcga tcgaacctga gaaggatgtc cgggccttca
     4201 ccacatcact ccgacagaca tttccaacca tcaagctctc ccccgagcaa gccgaccggc
     4261 tttttacaca gactgacccc cgcgtgctca gccttgctga tcgaggctat gtggcccgca
     4321 tccatccact cgaattgtgg acggttgcat tcctccggca acatcccgat tccggcaaat
     4381 ccgaactcgc caacgccagt gagcaggagt tgttggaggt gtacgcctgg ttatttaaga
     4441 ctcatcgcaa agccgcacag gatagtcgga ttcgacttat tctcgaacaa gaggcattca
     4501 tggaaatcca caaagcctgg aaacgggtcg gatatccctt tgcaacatta gtcccgtcgt
     4561 tagctaccgc aattggcagt tccgccgacc gcccggcggc tttgaccgaa cttatgggta
     4621 ttctggtcaa cgagggaaga aaaaccccca cggtgacaat tcggcaactg cactttgcgg
     4681 aaggaacccc gtttgaaaca cttgtcgcac ctcaagaacc aaaccaggaa caaattttga
     4741 acccgctggt cgctcaaatt ctccgccagg aacttagatc ggaagagcgt cgtgtaggga
     4801 aagagtg
//
LOCUS       DATIAL010000398         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_610654, whole genome shotgun sequence.
ACCESSION   DATIAL010000398 DATIAL010000000
VERSION     DATIAL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610654"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            677..898
                     /locus_tag="VLA60_12350"
     CDS             677..898
                     /locus_tag="VLA60_12350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HSF10198.1"
                     /translation="MRIQDIPEIDRLSTPEKILLVEELWDHIASDPSAIPIPQSHANE
                     LERRLHRQETQPGTLLSLEELQSRIDRRK"
     gene            895..1203
                     /locus_tag="VLA60_12355"
     CDS             895..1203
                     /locus_tag="VLA60_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10199.1"
                     /translation="MTYPFRFLPEVEDDAVAAYIWYEQKAPGLGEEFLRTFYGDAGKL
                     SQNPLLYPTLHLKFRRRLLRRFPYAIYFSSEEREIVVSGLFHCARDPRGIQSILQHRR
                     "
     gene            1314..1745
                     /locus_tag="VLA60_12360"
     CDS             1314..1745
                     /locus_tag="VLA60_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10200.1"
                     /translation="MNQTQNGGQWNLLGTFTLNPSMNPMVELSDQANGRVVADAVMVV
                     PSGVSTDHVTYTPTLTTAGTVDIYAKWTENVTRAQAVTYNVQHASGGTDIVVNQQQPS
                     AGWFRLGAFVMSPGQNHHVEVAGALEGVTVADAIRFVSAGA"
     gene            complement(1798..1977)
                     /locus_tag="VLA60_12365"
     CDS             complement(1798..1977)
                     /locus_tag="VLA60_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSF10201.1"
                     /translation="MQPVEFDAIVFQEETTYVAHCPELDVSSCRKTLDQARQNFKTAV
                     RLFLEEAEKLGTSHC"
     gene            2145..2501
                     /locus_tag="VLA60_12370"
     CDS             2145..2501
                     /locus_tag="VLA60_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10202.1"
                     /translation="MNQTQNTAQWNLLGTFTFNPASNPMVELSDQVNGPVVADAVMVL
                     PSGVSTDYVTYTPTLAIPGTVDIFAKWSESATRAQAVTYTVQHAGGPKYQPGFSWLNR
                     KPPSHRSILSFLFPGN"
     gene            complement(2573..2902)
                     /locus_tag="VLA60_12375"
     CDS             complement(2573..2902)
                     /locus_tag="VLA60_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10203.1"
                     /translation="MGTDPASLQRLKTFMDLIRRAKQVGAEPTFISTPTEHGDNINVA
                     ATIDGKQATAGILFWDVSVLQNNGLADTLDEEDLALGLNITDGLVEDAEKILTFVDTE
                     LAKLAST"
     gene            complement(3038..3253)
                     /locus_tag="VLA60_12380"
     CDS             complement(3038..3253)
                     /locus_tag="VLA60_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10204.1"
                     /translation="MGRKKSDDEIFPEMAAMLEGMQELMCDEDGKPLSPDHPQYQKLT
                     GLMENIATRAQKRNQQDPSTSGQHGTA"
     gene            complement(3308..3943)
                     /locus_tag="VLA60_12385"
     CDS             complement(3308..3943)
                     /locus_tag="VLA60_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HSF10205.1"
                     /translation="MKSGQTQISRIIGGILLISLLGWANVALSKIQGEYELMEGEPSQ
                     HEAGKVILFEFADFYCSHCHMFERVVGTKLKKEFGDKLEIRMVGFPVIPGKLPTAFEM
                     YNQAVTMGKGPEMKQLLFQSIHEKDIQVFDKTMRSLLLKEIGLDPTEFEAGLASGKPF
                     KTLAKGRTWGERIKVTHTPTVLLDGNIRVANLTAENLRTVIESILNQDSKS"
BASE COUNT         1010 a         1101 c         1193 g         1164 t
ORIGIN      
        1 actggttcag tttttatatt gtttttaggg ttagggataa tcctgttaat taagattttg
       61 caaaacaata aaaaggtaaa gctgtaggag aggatgtcgc aaagggggcc gatgcacggg
      121 aggagataag gaggcagaaa cacttccttt agatttttga gccggggttc gtccaggcag
      181 ccgaggtcct tttgtttcgg caaaaggacc caaaaccagt ggccctcagc gtgggtcaag
      241 agatgtcctc cgctgctcgc cgaatccagc ggcgggcaaa ctcgctgcgc tcagacaatg
      301 cccgccttaa ctcttgatcc ggctctgcgg ctcggccacg ctgaaagggc cgatttactt
      361 catcctcagt gcaccatcct ggcggcgcga ctgatttttt ggtgaatcag cagcagccta
      421 gtgcgggggg tggtaagagt tggggatttg ccatgaatcc gtggcaaaat tctcgcgtag
      481 ccatggcagg ggaggggaaa ggtatgccat tcagtcggta ccaacttttg gaatagccca
      541 aagcattcag taggttcatg cagaccattg agtcagccat caggaaattg acaaatgcgt
      601 tctgcctgtt tagcctttct gtggcgctac caagagctaa atctgcacgg ggaaccggga
      661 tgagaggaga acatacatgc gaatccaaga cattccagaa atcgaccgat taagcacgcc
      721 agaaaaaatt ctgctcgtcg aggagctctg ggatcatata gcatccgatc catccgccat
      781 tccaattccc caaagtcatg ccaacgaact cgagagacga cttcaccgtc aagagaccca
      841 acccggcaca ctgctctctt tggaagaatt acaaagcaga attgatcggc gtaaatgacc
      901 tatccctttc ggtttttgcc cgaagttgag gacgatgccg ttgccgcata catttggtat
      961 gagcagaagg cgccaggatt gggtgaggag tttttgcgta ccttttatgg ggatgccggg
     1021 aaattatcac agaatccatt attgtatccg accctacatc tcaaatttcg ccgtcgactg
     1081 cttcggcggt ttccctatgc catttacttc agttcagaag aacgcgaaat tgtggtatcc
     1141 ggtctttttc attgcgcccg cgatcctcgg ggcatccaat ccattctcca gcaccggcgg
     1201 tagttgagag ggtgtagctc tttaatcccc tcccgcgtgt cctccgcttc tcggccacac
     1261 cagatctatc cgtaggacga ttccaagacc tactggacgg agaaggggtg ccgatgaatc
     1321 agacacagaa cggcgggcag tggaatctcc tcggcacctt taccctgaat ccctcgatga
     1381 accccatggt ggaattgtcc gatcaggcca atggccgcgt ggtggccgat gcggtgatgg
     1441 tcgtgcccag cggggtcagc accgatcatg tgacctatac gcccaccctg accacagcag
     1501 gaacggtgga catctatgcc aaatggacgg aaaatgtcac ccgcgcgcag gcggtgacct
     1561 acaacgttca gcatgcgagt ggagggaccg atattgtcgt gaatcagcag cagcccagtg
     1621 cggggtggtt tcggttggga gcttttgtga tgtcgccagg acagaatcat catgtggaag
     1681 tcgcaggcgc cttggaaggc gtgacggtgg cggatgcgat acggtttgtg agtgcgggcg
     1741 cctagccacc ctctcagggg gtaaacagat tattagacat gggaaattac acctggtcta
     1801 acaatgcgat gttcctaatt tttcggcttc ttcgagaaaa agtcgcactg ccgttttaaa
     1861 attctgtcgt gcctggtcaa gtgtctttct gcaactggat acgtctaatt cagggcaatg
     1921 ggcgacatac gtggtttctt cctgaaaaac aatggcatca aactccacag gctgcatagt
     1981 agactcctat atcgtgaaag tcttgttgaa gaattatgcc tgcccactat accaggaggg
     2041 ttattcggca gaaagagggc tgacacttta gacttgtggg cagggagact gatctgcaaa
     2101 cagctaccac ccatcatgcc aatggtggca agatggtcgt tccgatgaat cagacacaga
     2161 acaccgcgca atggaatctc ctcggcacct ttaccttcaa ccccgcgtca aacccgatgg
     2221 tggagttgtc tgatcaggtc aatggcccgg tggtggccga tgcggtgatg gtgttgccca
     2281 gtggggtgag caccgattat gtgacctata cccccacact ggccatccca ggaacggtgg
     2341 acatctttgc gaaatggagt gaaagtgcga ccagagcgca ggcggtgacc tacaccgttc
     2401 agcatgcggg cggtccgaaa tatcaaccag gcttctcgtg gttgaatcga aagcccccat
     2461 ctcaccggag tattctctcc tttttatttc ccggcaattg aaggcagtga agcttggagt
     2521 cttatggttc tttgcgattg tggtgctgac aagctacata gaggtcgaga aattatgtgg
     2581 aagccaattt cgccagttcg gtatcgacaa atgtgaggat tttttcagca tcctcgacca
     2641 ggccgtccgt gatgttgagc ccgagagcga gatcttcttc atctaaggta tcggccaatc
     2701 cgttattttg taatacgctg acgtcccaaa ataatatccc ggcagtggcc tgtttcccgt
     2761 cgatggtggc ggccacgtta atattatcgc catgctcagt cggggtactg ataaaggtcg
     2821 gctcggcacc tacttgcttc gctcgtcgaa taaggtccat gaatgttttt aatcgttgta
     2881 gactggcagg gtctgtgccc attaatatct ttcctctctg gcgtgaaggt tcaactggtc
     2941 caccttcaat ttctggtttg ccaattcgac gaaaggccct cacgaaatca atcgaaggtg
     3001 tagttccaag gcgtagatcc tatgagccga ttcggtatta cgcggttccg tgttggcccg
     3061 aggtcgaagg atcttgctgg tttcgtttct gggctcgggt ggcaatgttt tccataagtc
     3121 cggtcagttt ttgatattgc gggtgatcgg gagagagcgg tttgccatct tcatcgcaca
     3181 tcagttcctg catcccttcg agcattgcgg ccatttcagg aaaaatttcg tcatccgatt
     3241 tttttcgtcc catggtgtcc ttcctttgct atcgattcga aatggattgg tacgttcatc
     3301 gtttacttta agatttactg tcctggttga gaatgctttc gatcaccgtt ctgaggttct
     3361 cggcggttaa gttcgccacg cgaatgtttc catccaaaag cacggttggc gtatgggtga
     3421 ccttgattcg ttccccccac gtccggcctt tcgctagtgt tttgaaaggc tttccactgg
     3481 caagtcctgc ttcaaattcc gtcggatcga gcccgatctc tttcagaagg agggaacgca
     3541 tggtcttatc gaagacttga atgtcttttt cgtgaatgga ctgaaagagg agttgtttca
     3601 tttctggccc tttgcccatg gtgacggcct gattgtacat ttcaaaggcg gtcggaagtt
     3661 tgccgggtat gacgggaaat cccaccattc taatttctag cttgtcgccg aattcctttt
     3721 tcagtttcgt tccgacaacc cgttcgaaca tgtggcaatg tgagcagtaa aagtcagcga
     3781 attcaaacag aatgaccttg ccggcttcat gttgagaagg ctccccctcc atcagttcgt
     3841 attcgccctg tatcttgctc aaggccacat tggcccatcc gagcaggctg attaggagta
     3901 ttcctccaat aatgcgggaa atttgcgtct gtcctgattt catgcatgat cctttcttga
     3961 tttcgatctt atttctggac tttttctctc gaatcctcgg agcctgttag ggtaacatgc
     4021 ttcgttcttg gaggcacgct cacgttagcc catcatatca gactgaacct agggtccttc
     4081 aaggtttctg aaaaggggct gttgaaaaaa gccgccagcg gcgttccctg ccttcgccgg
     4141 agcggcttcg cgcaggcagg tcgccatttt tccatgctca cgtactggaa attcgctctg
     4201 cgcgtacaaa tgactgcggc cttgccttcg acatgactgg tatggtgtct cagggcaggg
     4261 ctggacgaac ccttcgaaag gctcagggca tgcttttttg aaccgccccg aggcctctga
     4321 tatgcaacgt gcctgtgggt caatatgccc ttggcaatta ggagtattca acactcccgg
     4381 aaaatgaaca tgcctcgggg aagtccggaa aggtgagggc ttgttcgggg taggggattt
     4441 gttgaggatg attgagagaa tggtggag
//
LOCUS       DATIAL010000399         3388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_825767, whole genome shotgun sequence.
ACCESSION   DATIAL010000399 DATIAL010000000
VERSION     DATIAL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825767"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1044..2018)
                     /locus_tag="VLA60_12390"
     CDS             complement(1044..2018)
                     /locus_tag="VLA60_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10206.1"
                     /translation="MRKKLSFWMCLFALIIICGQTFSWIALAFAEESSPGTSQKQKTF
                     SFFKMVEESFFGDPTDPWRPISLSTFFSEGWDEPFDYPVKGSGGAPRQGWINSLDGVF
                     YRLWFLSYGHTQNFHDNGNQDFGRYTLFAPVSRRFQIRFDVPFLVSNRGGEQNGYHNN
                     FGDFVISPRFLLSETQDVSQIFTIAVRTPTGEKENGHDVFSITPEYQFWSNVYNTWVV
                     RGGIGTTFPTQSNRGRYSFNYNVAIGKYLTSHDQTPFGDLTLYVAANGFTTLDNRASD
                     VNFFSMTPGFRSHMGRDWYLLAGVEVPLTGPDSASFDYETNVWVMKVF"
     gene            2714..3070
                     /locus_tag="VLA60_12395"
     CDS             2714..3070
                     /locus_tag="VLA60_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HSF10207.1"
                     /translation="MSACDTIATELAAKGYVEIVAGSSDFYVTYHVRAREKTEIQPMP
                     GSMIGPGWGRGYAEVYQYEEGTLIIDLFHAKAQHLIWRGIAKGVVDWHAALERRAELI
                     DEAVKKNFAQFSPPHS"
BASE COUNT          887 a          787 c          826 g          888 t
ORIGIN      
        1 ggagcggaga ttcatcatgg gatgttcggt gtatggcttg tctcccccct catagcgaag
       61 gaacaaaacc aagcggggga tcggcacccc cacggcctcg tgtcgcaggg gagaggaagc
      121 aatgtgggca tgagtgagca tgtcattgaa tttttcctgt gggcaaggtg cctacttggt
      181 ttcagcgatt gtcggctgct ccgcgacgac agattttctg aacaaggtag tcagacagcg
      241 gcaaagcggc gattgcgatt attcgttgga atcatgaagc tgctgagtta ctattgttac
      301 aacaaggctc ctgcagcaag gttctaaccg catcgcccac atttttttga acgaagtcgg
      361 tggtttcgtt gacccccacg ggggaggcga aaaatcctcg agcccatatt tctccgctag
      421 cccaatatgt tttttttagc acgggaagga atttctcctt cagcctctgg ctcggcatac
      481 ttttcaatgt ccctcctact tctgagacca catattttgg cggaatggtc atatgcacct
      541 gcacgtgatc gcgatctacg ccaatttctt cgatatacca gtcgggatga aattccctca
      601 cttccaacaa tgccttcttg aaatactctg ccaacccggc ctaaaggatt tttcgccgct
      661 agcgcgtcac ccacaccaaa tggtactggg ttttgtaaac aacgtgggct tgtcgatgga
      721 gcttcatcgg ccccatcata ccagggggag ctgaagacgg ctcctggctc tcaccctcac
      781 ccttacaggt ggggaactct cgctaaatct gattttgttt gtgaatatca gtgtctgcca
      841 aaattgcact ttcgccacgg aacgttgccg ctgtatgcgg cttacaatac gaagatgggc
      901 aaattgctgg gcaagaccgc cgaccgccac accttgtctg agttcgtatc ttgaaatacc
      961 aacgcaagtg cctaaggggc ctctcctcaa ataagataag gtattttcaa catggatcaa
     1021 tcattgggga ttggttaaaa ccattaaaac actttcatca cccacacgtt tgtctcataa
     1081 tcaaagctgg cggaatcagg tcctgtcaga ggaacttcca caccagccag aaggtaccag
     1141 tctcgaccca tatgggaacg gaatcccggt gtcatgctga aaaaattcac atccgatgct
     1201 ctgttatcta aggttgtaaa tccgtttgct gccacatata acgtcaagtc cccgaagggc
     1261 gtttggtcat gtgaagtgag gtatttacca atggcgacgt tgtaattaaa tgaataacgc
     1321 cctcgattag attgtgttgg gaaggtggtc cctattcccc cgcgcacaac ccaagtgttg
     1381 tacacattcg accagaattg atattcaggg gtaatgctga agacatcatg cccattttcc
     1441 ttttctcccg ttggcgtgcg tacggctatc gtaaagattt gactgacatc ttgtgtttcg
     1501 gacagcagga atcttggact gatgacaaaa tcaccaaaat tgttatgata gccattttgt
     1561 tctcctccac ggttgctcac caagaacggg acatcaaaac gtatctgaaa gcgtctgctt
     1621 actggagcaa acagcgtgta acggccgaaa tcttggttgc cgttgtcatg gaagttttgg
     1681 gtgtggccat aactaaggaa ccacaatcga tagaatactc cgtcgaggga attgatccac
     1741 ccttgccgtg gggctccccc cgaacctttc acgggatagt caaagggttc atcccaacct
     1801 tcgctgaaaa aagtactgag cgatatagga cgccatgggt ccgtcgggtc tccaaaaaaa
     1861 gactcttcta ccatcttaaa aaatgaaaag gtcttttgct tttggctggt acctggtgag
     1921 gattcttccg caaatgccaa ggcgatccag ctaaaagttt gtccgcaaat gattatcaga
     1981 gcaaacaggc acatccaaaa actgagtttt tttctcattg attattcttt ctgccggtca
     2041 tgcgccggcg ggattttcca aacaggtaca tactacaact tgtggagtat gatttcacag
     2101 ctgtaaaacc cgagaagtat gtatgaaaag tcaatttcct gccatctttt tctgcgacag
     2161 atttataaat atttcttgag atggttggag gctgaaatcc ttaccaaatt tagacgatcc
     2221 gtcatttatg actcatgaaa tgagtacgtt acctccactc gggttatttg tccatgaata
     2281 cccatgttct ggacccgctg ccaccaaccg atccagaatt tccgactgct gaagcagcat
     2341 cgtagcattg ggtagactcg taaggtgtta gggcatcaga acctgacctc cgattgaatg
     2401 aagaaataat tagtattttg agccggtgga tttccaatag atcggtgggt gtgaattcac
     2461 ttctccgggc tccataaaga ccatcaaagg tttgattata agtgccggcg ggaatttggg
     2521 tgccactggc ataatcgaac atcgccatga ggcgaggaga ccagggcagc ataaaggtat
     2581 aacctatcga cacgtgttgg aagtaggcaa agtgatcgtt gccatccaca gtaccggcct
     2641 gcaagaccga ttcaacatca tagccgaaca atgcgacctg aggaggttga tagagcctta
     2701 ggccaagggt ggaatgtctg cgtgcgacac gattgcaacc gagcttgcgg caaaaggata
     2761 tgtcgaaatc gtcgccgggt cgtcagactt ttatgtcaca tatcatgttc gggctcgcga
     2821 gaaaaccgaa attcaaccta tgcccggttc gatgatcggg ccaggctggg gtagaggcta
     2881 cgcagaagtg tatcaatacg aagaaggtac actcatcatt gatctgttcc atgcgaaggc
     2941 gcagcatctc atctggcgtg ggattgccaa aggtgtggtt gattggcatg cagctcttga
     3001 acgaagggcc gagttaatcg atgaggcagt caagaaaaat tttgctcaat tttctccccc
     3061 gcattcttag actgtctgtg tgcttgccag acaaaaacgt ttccgaagga ttctctctgg
     3121 aacttagtcg attgaatttc tttcatactc ctaaacagga ggttgtgtta gagattgaaa
     3181 acgaacacgt ttaaatgggg tgaaatgaaa tgaccggaat agaggtatgt gtatacctgc
     3241 tttagcaaat taaaactcta tccccggagg tgtttaatct ttatgcttgg agggagacga
     3301 acgtgatctt atccgagtcg ggaggaatta tctgaaggac tcgccggaag atgtgtatgt
     3361 gtatcgcttg accgaaccga tcgagaat
//
LOCUS       DATIAL010000400        11176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1256590, whole genome shotgun sequence.
ACCESSION   DATIAL010000400 DATIAL010000000
VERSION     DATIAL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256590"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            867..2816
                     /locus_tag="VLA60_12400"
     CDS             867..2816
                     /locus_tag="VLA60_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10208.1"
                     /translation="MPSRVEKPQLTTLRIFIVDDSPSDRSTYTRWIRRTASFRLDIQE
                     METGTQAWEAWRISPPHCLLLDDQLPDMTGLEFLAKVRGPHSQSKTPIIFLTGYGNDE
                     IAAQAIQLGAQEYFNKSTLTERFLWHHIEQAIKQVITIPRIRSLERQASIILQSSSDG
                     ILVVGHDGRIRYSNPAAEQLFQKTSEQLTGSPFGFPISANQTTEIGIMGESGQPTPVE
                     MRVVPIEWDEEPAYLASLRDVTEQRRAEDERRRHEIERQYSQKLESLGVLAGGIAHDF
                     NNLLMTIVARSGLALRALPPDAPAREHLDFIEKAGLRGGELANQMLAFAGQTRLNFQV
                     INLPKLLEDMTPFLRATLSKRLTFEYDLAPSLPPIRADQAQLRQIIMNIVINASEAYG
                     EGDGVITIRTFEWEPTIENFQSWYVIGELPDNRSVALTIQDTGCGMTSDTIPKIFDPF
                     FSTKLPGRGLGLAALLGLAQAHAATIAVRSQPDVGTEFTLLFPATTPSPSTKKRPVFS
                     IPTQHASLTKKPKVLVVDDEEDVRVACSMIFEEMGLQTLVASDGQIGIQVFQQHQHEI
                     MVVLLDLTMPNMDGQQFLEHIQGLNATIPVILSSGYSEEEAMKRFDNPNMAAFIQKPY
                     QVETLIAKVEEIRQKQITSSNKPGL"
     gene            3341..4726
                     /locus_tag="VLA60_12405"
     CDS             3341..4726
                     /locus_tag="VLA60_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HSF10209.1"
                     /translation="MTYSHDLIIIGGGSAGYAAARTAQAEGADVGIVDQGPLGGLCIL
                     RGCMPTKTILRSSDLAALIRRAPEFGLHSTNLQVNLAAIIERKNRLIKAFAEDRIQAL
                     QHPRFALYQEHAHFVSPTTIQAGPHRLSAKAYIIATGSVVSHIPIPGLEEAGYLTSDE
                     ALELETLPESMIVLGGGPVALEFAQFFSRIGVMVFLIQRSAHILSDSDEDLVRPVEAH
                     LQAEGMKMYTNTQIQYISTSGGQKTIHFLHQGQRKEVTAISILQALGRRPNIDGLNLE
                     AAQVAHQTNAILVNKDMRTSQPHIFAIGDVNGGYEIVHIAIEEGEIAGWNAVHPNLPP
                     KHFDSRLKTQVVFTDPQLASVGLSERECLDRQLPYLVASYPFNDHGKSLCLGETYGHV
                     KVLCHPQSGEILGGHIVGPEASELIHELIAIMYFRGTVHDLLRIPHYHPTLAEILTYP
                     AEELVGKLPSV"
     gene            4946..5683
                     /locus_tag="VLA60_12410"
     CDS             4946..5683
                     /locus_tag="VLA60_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HSF10210.1"
                     /translation="MSHPSATPTDSPNPLMGKVVIGSILLAGIGAFFYFDLGHYLSLD
                     ALKANRDILLAYTASHYEIAAAVFILIYILQTAFSLPGGAILTLTGGFLFGSLMGTLF
                     VNIGATAGATLAFLAARYLLHDWVERKFGDRLGTIQEGFANNAFNYLMTLRLIPAFPF
                     FLVNLVSGLTRVNLGTYVLATSIGIIPGSFVFAFAGRQLGTINSLSEIASPPVLLAFT
                     LLGLLALMPVAYQKWKKTQSGIINPKS"
     gene            5718..7268
                     /locus_tag="VLA60_12415"
     CDS             5718..7268
                     /locus_tag="VLA60_12415"
                     /inference="COORDINATES: protein motif:HMM:NF004991.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HSF10211.1"
                     /translation="MNTPQSPAHNGLVFPQDHYNQELVNNVHPPQWRNPTPSGRYNLV
                     IIGAGTAGLVTAAIASALGAKVALIERHLMGGDCLNVGCVPSKGVIRAAKAWQAVREA
                     EQFGVHISGEIKQDFGAAMARMRKLRARISHVDSAHRYTKLGVDVFIGNGRFTSSSTI
                     DVEGTTLQFAKAVICTGARATAPPIPGLANTTYLTNENIFSLTELPQRLGVIGAGPIG
                     CELAQSFVRFGSQVFLVEAMHGILPNEDRDAADIVEQSMLRDGVQLLCCGKDLNIHSA
                     NGAKHLVVDSHGTHYDIPVDEILVGVGRSPNIEGLELERVGVEYDKTGVKVNHRLQTS
                     NSKIFAAGDICSPFKFTHTADAQAQIVIQNALFPHPFGLGYGSTEHLVIPWATYTQPE
                     IAHVGLYEKDAKDKNIPIDTFTFHLNEVDRAILDGEDQGFARVHVKKGTDSIVGATIV
                     AAHAGDMISEFTLAMKGGLGLNTIAGTIHPYPTQAEVVKKSANAWRKTTLTEGKKQFL
                     RKLFAWTR"
     gene            7387..8160
                     /locus_tag="VLA60_12420"
     CDS             7387..8160
                     /locus_tag="VLA60_12420"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HSF10212.1"
                     /translation="MTRITPRPAFFSVPTVLFGLLSLTHSNAVIAANPDHLAVGNFSI
                     ATPGEPFPQEWKPLTFDNIPEQTQYELVKDDQQVVVKAISRQSSSGLTREISIDPKEY
                     PVIAWRWKVENILQKGDVAQKSGDDYPARLYITFQYDSSQVGFFEKAKFETIKFIYGQ
                     YPPIGAINYIWESKSPIGTMVPNPYTDRVYMFVTESGSAKLNQWVTEERNIYEDYKKA
                     FGEDPPNISGVAIMTDTDNTKESAVAYYGDIVFKKSGRK"
     gene            complement(8278..8853)
                     /locus_tag="VLA60_12425"
     CDS             complement(8278..8853)
                     /locus_tag="VLA60_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HSF10213.1"
                     /translation="MALKDSTMLPLGTPLPHFKLPDVRTGHMVSPESFVGKHAILVMC
                     ICRHCPYVVHVQEELAKLGRDYQKKNVGIVAISSNDVKRYPQDSPTRLKEMAEELDFR
                     FPYCYDESQEVAKALTAACTPDFFVFNEERKLVYRGQLDDSRPGNGKPVTGRDLRMAL
                     EAVLDKKPVSVVQKPSAGCSIKWKTGNEPDY"
     gene            complement(8982..9281)
                     /locus_tag="VLA60_12430"
     CDS             complement(8982..9281)
                     /locus_tag="VLA60_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HSF10214.1"
                     /translation="MKHKLMRYMVGNFSCQEIAQLITDYLDGSLTLTERIRFQMHLGL
                     CFACRNYLRQMKYTIATLRQLPPEPVPQHVREELLERFRAWKQRSPQSSVKSETD"
     gene            complement(9299..9976)
                     /locus_tag="VLA60_12435"
     CDS             complement(9299..9976)
                     /locus_tag="VLA60_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF10215.1"
                     /translation="MLISPSPLTVLDKLKLDEPGLVRALQAGDEQVWATVMDWYSGSL
                     LRLAMAYVPSRAVAEEVVQETWMGVFEGIHRFESRSSFKTWLFRILTNRAKTRGIRES
                     RYEPSGLSTSSPDADEGPSLEDSLFVAEGSRMGHWKNPPHDWEPDTPERVLLSKECRA
                     AIETAIENLPATQRQVMTLRDIEGVSSEEVCNILEISETNQRVLLHRARTRVRRELNP
                     YVQGKNT"
     gene            complement(10219..10860)
                     /locus_tag="VLA60_12440"
     CDS             complement(10219..10860)
                     /locus_tag="VLA60_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HSF10216.1"
                     /translation="MSRRSIGIPCTTVPKSTQGNGLSRLQEIADRRSIPGVLLFSGSG
                     DVLFMNAEAEMLSRQIMGDAYDAEARSVWPADVLELCESLRTLLDNPENQLVEGQHEL
                     RRVTGDVKSPVLLRGFPLAAHTEKDEACMLIIMEKIGRERRVVSNQAREQYRLTSREV
                     EIVKYISEGWTNKEIARHLEISEHTVKEHVRHLLKKTKTTTRTGILAQIFQDT"
BASE COUNT         2934 a         3084 c         2561 g         2597 t
ORIGIN      
        1 ctacaagtca ttgccgaagg agtggaatca caacaacaag tcgatttcct ggaagaatat
       61 ggatgcgatg cgatgcaagg gtattggatc gcgccgccac tccccgccaa tgaaagcacc
      121 cagcacatgg tgcacatggc ataaggacag ttggctcaca ccttctctct ctatccttaa
      181 aatttcggtc atcctttctc tgatattccg ttctcactct tcactctgaa tttcccaaac
      241 agttgttgtc tagcaatttg cttctgaatt ctcttgtttc ttgcagattt tcctatcagt
      301 ctgattcagg gccgacgtaa gaagaggggt ctaggccctc tactactcat ggcaccgctc
      361 ggtgtcggca cggagcgctc taatattttc gtgaccgtgg caacccatat tggaatcatt
      421 acatcagtat tactcagaat catcagcagg acatatttca gatattggag cgattgcacg
      481 gccgtcataa attcggagcc gggaacggtc cccgactcac cattgtgaac aaaccctcaa
      541 acgccctggc ggaaaaattt ccctagacag gattatggtt accatgctgc ccatctcacc
      601 ggtgaatgcg agttcaaatc ctggaattag gaagatgcca acagttatat tgggaaacct
      661 tttgaaaacg tttacttatt taaagctatg gtaaagttaa aaaaataccg gtttacatta
      721 ggcgtccact cgaaactggg caaaaaagaa caccaaatga ataatcccgc gatgatggag
      781 ggccatgcta ttgattgacg aatacatttt tgcctttcct cttaatctca gttgtttcac
      841 tttcattcat aggatcgccg tcaaccatgc caagccgtgt tgaaaaacct caactcacca
      901 ctctccgaat ttttatcgtc gatgatagcc caagtgaccg atccacctat actcgatgga
      961 tccggcgcac agcctcattt cgcctagaca ttcaagaaat ggaaacagga acccaggcat
     1021 gggaagcctg gaggatctcc ccgcctcact gtctgctttt ggatgaccaa ttaccggata
     1081 tgaccggatt agaattcctg gcgaaagtac ggggaccgca ctcacagtcg aaaactccca
     1141 tcatttttct caccggatac ggtaacgacg aaattgcagc tcaggccatt caactgggcg
     1201 cacaagagta tttcaacaaa agcaccctaa cagaaagatt cctctggcat catatagagc
     1261 aagcgatcaa gcaggtcatc accatacctc gcatacgctc tcttgaacgt caggccagca
     1321 tcattttgca atcatccagc gatggaatcc ttgttgtcgg acatgacgga cggatccgtt
     1381 actctaatcc tgcagcagag cagctcttcc aaaagacatc cgaacaactc accggatccc
     1441 cctttggttt cccgatctcc gcaaaccaga cgacagaaat aggcatcatg ggcgaatccg
     1501 gacaaccgac tcctgtagaa atgcgagttg tccctattga gtgggatgaa gaacccgcct
     1561 atttagcctc actcagggat gtgactgaac agcgaagggc cgaagacgaa cgacgccgtc
     1621 atgaaatcga acgccagtat tcacaaaaac tggaaagtct gggagtgctc gccggaggaa
     1681 tcgcccatga tttcaacaat ctcctgatga ctattgtagc ccgctccggc ctcgctcttc
     1741 gcgctcttcc tcctgacgct ccagctcggg aacacttgga cttcattgaa aaagccggtc
     1801 ttcgcggtgg ggaattggcc aatcaaatgc tggcatttgc cggacagacc cgcctaaact
     1861 tccaagttat taatcttcca aaactcctcg aggacatgac cccttttctc cgggcaaccc
     1921 tttcaaaacg actaacgttc gaatacgatc ttgccccctc ccttcccccc attcgagccg
     1981 atcaagctca attgcgccaa atcatcatga atattgtgat aaacgcatca gaagcctatg
     2041 gagaaggaga tggagtcatc accatccgta cattcgaatg ggagcctaca atagagaatt
     2101 ttcagagttg gtatgtgatt ggagagctcc cggataaccg aagtgttgcc ctgaccattc
     2161 aggacactgg gtgcggaatg acctcggaca caatcccaaa aattttcgac ccgttttttt
     2221 cgaccaaact tcctggacga gggcttggac tcgcagcgct tcttggactc gctcaggccc
     2281 atgccgcgac cattgccgtc cgaagtcaac ccgatgtcgg tacagaattt acgttactgt
     2341 ttcccgccac cacgcccagt ccttcaacaa agaaaaggcc ggtcttctca attcccactc
     2401 agcatgcctc actgacaaaa aaacccaagg tgttagtggt tgatgacgaa gaagacgttc
     2461 gggtggcgtg ttcgatgatc tttgaagaaa tgggattgca aaccttggtc gcctcggatg
     2521 gacaaatcgg cattcaggtc tttcagcaac atcaacacga aattatggtc gtgctcttag
     2581 atctcaccat gcccaatatg gacggccaac aattcctcga acatattcaa gggcttaacg
     2641 caacgattcc ggtgatactg tcgagtggat attccgagga ggaagcgatg aaaagatttg
     2701 acaacccgaa catggcagcg ttcattcaaa aaccctatca agtggaaaca ctcatcgcaa
     2761 aagtcgaaga gatcaggcag aaacaaatca catcctccaa caaacctggt ctgtagaaag
     2821 cgcccacctc catgctccgc catcttccac ctcgcatgac agctgagaac gacaacttcc
     2881 gcgtaggtat cgttcacgcg tcgaggatag tcccagatga tttcgaaaca aggcggaaat
     2941 gacaatcttc acactctgag gatccaactc ccctgcctat cggaagtcca gagtcccttc
     3001 ttcatattgg ctcgtgccga atgcagtctc caaaaatctg gatcagtccg cgaggcttag
     3061 tcaaacgact caacggcttc cattcaatat ccagactgat acgggtcaag gcccgtcaaa
     3121 ttcaggggag tgccatggga tgatctaatg aatagggaaa gcagaggaac ggatgtcgct
     3181 acacagccac tttacatgac caatcaaatc cgaaggacac ggagcgcagg ctgtctccga
     3241 caactgcctt tcttccttgt aactgttcat gctacatatt tctactcgga attcgaccat
     3301 gcggattttc tcaatttatt cataagcaca aggcttccac atgacctatt ctcatgacct
     3361 catcattatt ggagggggat cggcaggcta tgcagccgct cgcaccgctc aagcggaagg
     3421 agccgatgtc gggattgtcg atcagggtcc gttaggaggc ctctgcatct tacgaggatg
     3481 catgcccacc aaaaccattc tccgctcatc cgatctcgcg gcgctcatcc gtcgggctcc
     3541 ggaattcggg cttcactcaa cgaaccttca agtgaacctc gcagccatca ttgagcgaaa
     3601 aaaccggtta atcaaggcat ttgctgaaga tcgcattcaa gcactccaac accctcgatt
     3661 tgccctctac caggaacatg cacattttgt ttcgccgacg accattcaag ccggcccgca
     3721 tcggctatcc gcgaaggcct acatcatcgc caccggttct gtggtgtcgc acattcctat
     3781 tccaggattg gaagaagcag gataccttac cagcgatgag gcattagaac tggaaacgct
     3841 tcctgaatcg atgattgttc taggcggcgg gccggtcgca ttggaattcg cccaattttt
     3901 ttcccgcatc ggagtcatgg tatttctgat tcaacgaagt gcccacattc tctcggactc
     3961 ggacgaagac cttgttcggc cggttgaagc acaccttcag gccgaaggca tgaaaatgta
     4021 taccaacacg caaatccaat atatcagcac gtccggtggc cagaaaacca ttcacttcct
     4081 ccatcaggga caaagaaaag aggttaccgc catctccatc ctccaggccc tcggccgccg
     4141 cccaaatatc gacggtctca atttggaagc ggctcaggtt gcccatcaaa cgaatgccat
     4201 tttagtcaac aaggacatga gaacatccca accacatatt tttgctattg gagatgtcaa
     4261 cggcgggtat gaaatcgtcc atattgccat cgaagagggc gaaatcgccg gatggaacgc
     4321 cgttcatccc aatctccctc ctaaacactt tgattcccgc ctcaagaccc aggtcgtctt
     4381 tactgatccc cagttggcca gcgtgggact gtccgaacgg gaatgcttgg accgtcaact
     4441 cccctatctt gtcgcttctt acccctttaa cgaccatggc aagtctctct gcctgggaga
     4501 aacgtatgga cacgttaaag tgctctgtca tcctcagtcc ggtgagattc tcggtggcca
     4561 tattgtcgga cccgaagcct ctgaactcat ccatgaactg attgctatca tgtattttcg
     4621 tggaacagtc cacgatcttc tccgcatacc ccattaccat cccactctcg ctgaaattct
     4681 tacctatccc gctgaggaac ttgtcggaaa attaccctcc gtttaggata accgtgcaca
     4741 cttaaaagag cccctatgtc gggatgactc ggacagtggt tacctaagag aagggaatcg
     4801 ctctcttcac tattcgaatc tttctcgtaa caaatgcccc tgaagcgcga ctacagcaca
     4861 atatcgccga agacgccgct ggataatctg gcgtaaggtc cacaattgga gaattgccat
     4921 gaaaacccgc ataatggagc ctcacatgtc ccatccgtcc gccaccccaa ccgactcgcc
     4981 caatccattg atgggaaagg tagtcatagg aagtattctg ctggcaggca tcggtgcgtt
     5041 tttctatttt gatctgggcc actatctctc actggatgcc ttaaaggcca atcgagacat
     5101 cctattagcc tataccgcct cacattatga aatcgcagcc gcggtattta ttctcatcta
     5161 tatcctccag acagccttct cactccccgg cggagccatc ctcacgttga cagggggatt
     5221 tctatttgga agcctgatgg gaacgctatt cgtcaatatc ggagccaccg ccggagccac
     5281 gctcgccttt ttggccgccc gctatctgct ccacgattgg gtggaacgca aatttggcga
     5341 ccggctcggg accatccaag aaggctttgc caacaatgcg tttaattacc tcatgaccct
     5401 tcgactcatt ccggcctttc ctttttttct cgtgaacctg gtctccggcc tcacccgtgt
     5461 caacctcggc acctatgtcc tggcgacgtc cattggcatt attcccggca gctttgtttt
     5521 tgcctttgcg ggccgccaat tgggcacaat caattccttg agcgaaattg ccagcccacc
     5581 ggtcttactc gcctttacgc tcctgggttt gttagccctc atgccggtgg cctatcagaa
     5641 atggaagaag actcaaagtg gaatcatcaa tcctaaatcg taaatgactg aaccctttat
     5701 caaactagag gcctcagatg aacaccccac aatcaccagc ccataacggt ttagtctttc
     5761 cccaggacca ctataaccag gagttggtca acaatgtgca tccgccccaa tggcgcaatc
     5821 ctacgccgtc cggacgatat aacctcgtca tcatcggagc cggcacagcg ggattagtca
     5881 ccgctgcaat tgcgtcagcc ctcggagcca aggtggcgtt aattgaacga cacctgatgg
     5941 gcggagactg cttgaacgtg ggatgtgtgc cctccaaagg cgtcatacgg gcggccaaag
     6001 cctggcaggc cgtcagagag gccgaacaat ttggggtgca tatttccggt gaaatcaaac
     6061 aggatttcgg tgcagccatg gcccgcatgc gtaaactgcg tgcccggatc agtcatgtcg
     6121 actcagcgca tcgttatacc aaacttggag tggatgtctt tatcgggaac ggcaggttca
     6181 ccagctcaag caccatcgat gtggagggaa cgacattaca atttgccaaa gcagtcatct
     6241 gcacgggtgc ccgcgccacc gcaccaccaa ttccaggctt ggccaacaca acgtatctca
     6301 ccaacgaaaa catcttttcc ctaacggaac ttcctcagag actgggagtg atcggggccg
     6361 gccccattgg ctgcgagctg gcccaatctt ttgtccgatt cggcagccag gtgtttttag
     6421 tggaagccat gcacggcatc ctccccaatg aagatcgcga cgctgccgac atcgtggagc
     6481 aatcgatgtt gcgggacggc gtgcaactgc tctgttgcgg aaaagacttg aacattcact
     6541 cagcgaacgg cgccaaacac ctcgtggtgg actcgcatgg cacacattat gacattcctg
     6601 ttgatgaaat tctggtcgga gtcggccgca gtccgaacat cgaagggttg gagctggaga
     6661 gggtcggggt cgaatacgac aaaaccggcg tgaaggtgaa ccaccggctt caaaccagca
     6721 attccaaaat atttgcggca ggagatatct gctctccatt taagttcacg catacggccg
     6781 atgcgcaagc gcaaattgtc attcagaacg ccctatttcc tcatccgttt ggcctcggct
     6841 atggcagcac ggagcacctg gtgattccct gggcaaccta tacccagccg gaaattgccc
     6901 atgtcggtct gtatgaaaag gatgcgaaag acaaaaacat tccgatcgac accttcacat
     6961 ttcacctcaa cgaagtcgat cgggctattc tcgatggaga agaccaaggc tttgccagag
     7021 tgcacgtcaa aaaagggacc gattcaatag tcggagccac catcgtagcg gcccatgcgg
     7081 gcgatatgat tagtgaattc actctagcca tgaagggagg cctgggactc aacaccatcg
     7141 ccggcaccat tcacccctac ccgacccaag cggaagtcgt caaaaagtcg gcgaacgcct
     7201 ggcgaaaaac caccctgacg gaaggaaaga aacagttcct ccgcaaactc tttgcatgga
     7261 cccggtaaga tcatgtccta tcaagcacct aaccctagta tgttgtggcc cgttgaactg
     7321 ctcagggcga acggagtcgg tcaacaaatg attcaaacac cactcaggct ttagcggaaa
     7381 tcagacatga cacgcatcac acctcgaccc gcctttttct ctgtccccac agtcctcttc
     7441 ggactcttga gcttgaccca ttcgaacgcg gtcatcgccg caaatcccga tcacctcgcc
     7501 gtcggcaact tctccattgc tactccggga gaaccgtttc ctcaagagtg gaaaccactg
     7561 acctttgaca acatccccga acagacccaa tatgaactgg tcaaagacga ccagcaggtg
     7621 gtggtcaagg ccatcagtcg tcaatcatcc tcgggcctga cacgggagat ctcgatcgac
     7681 ccaaaggaat atccggtaat tgcgtggcga tggaaagtcg aaaatattct gcagaaaggg
     7741 gatgtcgctc aaaaatcagg agatgactat ccggcacggc tctacattac cttccagtat
     7801 gacagcagcc aggtcgggtt ttttgaaaag gcgaaatttg aaaccatcaa atttatttat
     7861 ggccaatatc ctcccattgg cgccatcaat tatatttggg aaagcaaaag ccccatcggc
     7921 acgatggttc ccaatcctta tacggaccgg gtctacatgt tcgtcacgga aagcggaagt
     7981 gccaaattga atcaatgggt aaccgaggaa cgtaatatct atgaagatta caagaaagcc
     8041 tttggagaag atccccccaa catctcagga gtcgccatca tgaccgacac ggataacacc
     8101 aaagagtcgg ccgtcgccta ttacggggat attgtgttta aaaaatccgg aaggaaatga
     8161 gagtaaaaga gtgaaaagaa gacagcagga gagaaaaatt tcctgaatcg acccacttaa
     8221 ggaaatcgtt gcactctact tctcatgcct caacgttccc tcctcacaca ttgcgtatca
     8281 ataatccggc tcattcccgg tcttccattt aatactgcag cccgcactag gcttttgcac
     8341 cacagaaaca ggtttcttat ccagaacggc ctccagggcc attcgtaaat cccgcccggt
     8401 cacgggcttc ccgtttcccg gtcggctatc atccaattgt ccccgatata ccagtttgcg
     8461 ttcttcatta aacacaaaaa aatccggtgt acaagccgcg gtcaaagcct tcgccacctc
     8521 ctgagattcg tcataacaat aaggaaaccg aaaatccaat tcctccgcca tttccttcag
     8581 tcttgtagga gaatcctgag gataacgctt cacatcgtta ctactgatcg ccacaatacc
     8641 aacatttttc ttttgataat ctcgaccgag tttggccaat tcctcctgca catgaacgac
     8701 ataagggcaa tgtcgacaaa tacacataac cagaatggcg tgtttcccga caaaactttc
     8761 aggggacacc atatggcctg tccgaacatc cggtagttta aagtgaggca agggcgtccc
     8821 cagcgggagc atcgtcgaat ctttcaaggc catggtttac tcctttgggt taatcaagac
     8881 tgaggggttc ctgtgaacga gtacagtcag aaagcttgaa gtcctgttta ccacatcagg
     8941 tggaaaggga gaagggggcc accggtcatc actgcaatga attaatcggt ttcagatttg
     9001 acggaagact gaggggaacg ctgcttccag gctcgaaacc gctcaagaag ctcctctctc
     9061 acatgttggg ggacgggctc agggggcaat tggcgtagag tcgcaatggt atatttcatc
     9121 tgacgtaaat aattacggca ggcaaagcac aggcccaaat gcatttgaaa tcgaattcgc
     9181 tccgtaagag tcagcgaccc atccaggtaa tcggtaatga gttgggcgat ttcctggcag
     9241 gaaaaatttc ccaccatata ccgcataagt ttgtgtttca aatttgtatc cattgtcctt
     9301 aggtgttctt tccctgaaca tagggattaa gctcccggcg caccctggtg cgcgcccggt
     9361 gcaacagcac ccgttgattg gtctcactga tctcaaggat gttacagact tcttcagagc
     9421 tgacaccctc gatatcgcgc aaggtcatca cctgacgctg cgtagccggc aagttttcaa
     9481 tcgcggtttc aatagctgcc cggcattcct ttgacagtaa tacccgttcc ggtgtatccg
     9541 gttcccagtc gtgaggtgga tttttccaat gacccattct ggaaccttct gccacgaaaa
     9601 gagaatcttc caaggaaggc ccctcatcgg catccggaga agaagtgctc aacccggacg
     9661 gctcatagcg actctctcgt attcctcgtg ttttggcccg attcgtcaaa atccggaaaa
     9721 gccaggtttt aaaggatgac cggctttcaa accggtgaat gccttcaaat acccccatcc
     9781 aggtttcctg cacgacttct tcggctacgg cccggcttgg gacataggcc atggctaacc
     9841 gcaataacga accggaatac caatccatca cagtcgccca cacctgctca tcaccagctt
     9901 gaagagcccg aaccaaccct ggctcatcaa gtttgagctt atcgagcaca gtgaggggag
     9961 agggagaaat gagcaacctc tgaaaatttg aagcgacccc aaaacaggaa ccagaggatt
    10021 atccttagcc tgacagtcac aatcaagagt ttcctcaaaa gaaatcccgt ctggcagatc
    10081 catccttcta agcagacaca aggagccaac aggaaaaaac aggcaggtac tgaagattaa
    10141 caggcaaacg gattgttgca gatggcagga tcgaagggaa aaagagagac gaggcagtcg
    10201 ggcgtacgcc gggatgcgtt aggtatcctg aaagatttgg gcaagaatgc ccgttcgagt
    10261 agtggttttc gtttttttca gaagatggcg aacgtgttcc ttcaccgtat gttcgcttat
    10321 ctccagatgt cgagcaatct ctttattggt ccatccctca ctgatatatt tcacaatttc
    10381 tacttcccga ctggtcaaac gatactgctc cctggcctga ttagacacca cccgccgttc
    10441 acgtccgatt ttctccatga tgatgagcat acaggcttca tctttttctg tatgggccgc
    10501 taatggaaaa cccctcagta atacgggcga tttcacatca cccgtcactc gtcgcaactc
    10561 atgctggcct tcgaccaact ggttctcggg attgtctaat agagtccgaa gggattcaca
    10621 taactccagg acatccgcag gccacacact tcgcgcctcg gcatcatacg catctcccat
    10681 gatttggcgg cttaacattt ctgcctcggc attcataaac agcacgtctc cggaccccga
    10741 gaagagcaac accccgggaa ttgatcgtcg gtcggcaatt tcctggagcc gggacagacc
    10801 atttccctgg gtggattttg gaacagtcgt acaaggaatg ccgatagatc gtcgactcaa
    10861 ggtatactcc gtggatcagg ttatgtagcg atgtaaaatt cccttccgac agcccgcaag
    10921 ggagcaacaa ctcacggtgc ctgatgcccc gcgaccgcta cgattgatct taatgaactg
    10981 ttttcggagt gtgacagaag aaacttaaat aaacggaggg ggaggataaa gaaaacttcc
    11041 aagatgcggc agaaacccgt gtggccatga aaactgaagc ctttcttgct tatcccccat
    11101 gctgcaagaa ggaaaacgcg aggctccaag tgaggaatct tttttcaagc cggattccgg
    11161 ttcggaaata ccaccc
//
LOCUS       DATIAL010000401         3646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_74105, whole genome shotgun sequence.
ACCESSION   DATIAL010000401 DATIAL010000000
VERSION     DATIAL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74105"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..343)
                     /locus_tag="VLA60_12445"
     tRNA            complement(257..343)
                     /locus_tag="VLA60_12445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(307..309),aa:Leu,seq:caa)
     gene            complement(471..2687)
                     /gene="recG"
                     /locus_tag="VLA60_12450"
     CDS             complement(471..2687)
                     /gene="recG"
                     /locus_tag="VLA60_12450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HSF10217.1"
                     /translation="MPTHSSLSEIPIQFAKGVGPRRARLLEKLAVQTLEDAFWFLPWR
                     YENRLEVLPIGNLLPGMKATIKGRVQKVWVKTTYRRRLVVVTVTVRDETGLIECVFFN
                     QPYLEKTFVPGVSLLLTGPVLSNSSGSSPMTMRGPEYELLDEDELPDMAGGRIVPVYH
                     ETNGLSSKQIRRIIRSIFDHYADQLQEMLPLTMRAQLGVPPLPDALPVLHFPNQERSV
                     EALNAYATPEHQRLAFEELFLLQLALAMRRQEQAQDIEGIAFAVRNRLVEQLKTVLPF
                     SLTSAQERVIKEISHDMGRPTSMNRLLQGDVGCGKTVVALHAIVMACGSGYQAALMAP
                     TEVLSEQHYLSLLPLFDALGVRCLLLKGGQTGRERGKILAGMQSGQVQVVVGTHALLQ
                     PDVHFAKLGLVIVDEQHKFGVLQRAQLRGKAPWHPDVLVMTATPIPRTLAMTLYGDLD
                     VSVIDQFPPGKKPVKTMLFPTGQRKDAHRLMRKELEAGRQAYVVYPLVEPSEKIDLQA
                     AIEAAEQLREECAPFRIGLLHGRLPSREKQVVMAQFKKKEIDVLVATTVVEVGLDVHN
                     ATVMLIEHAERFGLAQLHQLRGRVGRGLYQGYCVLVHGLGKIPPYSQQIPLKLESSRM
                     SLDKIGRSSQGEAFSPSTARRRLGVFAQCEDGFALAEEDLSIRGPGHVLGVKQWGEMD
                     FRVADLARDTVLLIKAKQMAGEILECDPRLMLPDHRILKETLFRKWGEKFALGSIG"
     gene            3544..>3646
                     /gene="rpmB"
                     /locus_tag="VLA60_12455"
     CDS             3544..>3646
                     /gene="rpmB"
                     /locus_tag="VLA60_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HSF10218.1"
                     /translation="MAFSCDVCGKNRLVGNNVSHANNRTKRVFRPNLR"
BASE COUNT          950 a         1027 c          797 g          872 t
ORIGIN      
        1 attgctaatc caaaataccc taccttccgc atgtggctcc tccttggaag tgtgagtgaa
       61 taatcaaaaa aacgaacgaa ttcaaatata cgattttatt tcatgatcaa gggcaccaac
      121 tatagtcaag gcggtagccc ctgtcaagtc aattcttggc tttgaatcat aggtcaacat
      181 acccgaaatt tcacttaaag gcttacactg ttttttccat tttttctcta ttttcattga
      241 accctaaaga cagtaatggt gcccggaggg agggtcgaac tcccactctc gtgaaagaac
      301 cggattttga gtccggcgcg tctgccagtt ccgccatccg ggcaaagcca ttttcacctg
      361 cgatatctac cacgaacctc tatgcaggtc aaacaggaaa tgctggcccg cttctccaat
      421 aaataaacca tagcggggca ttctcatttc ttccccatta ttagatgtcc ttatccaatg
      481 gaacccagcg cgaacttttc accccatttt cgaaacaatg tttccttgag gatgcggtgg
      541 tcgggcagca tcaaccgtgg atcacattcc aatatttctc ctgccatttg tttcgctttt
      601 attaacaaca cggtatctcg agccaaatcc gccacacgaa aatccatttc cccccattgc
      661 ttgaccccta agacatgccc aggcccacga atactcaagt cttcttccgc cagtgcaaag
      721 ccgtcttcac attgcgcaaa caccccaagg cgccgcctcg ctgtggaagg ggaaaacgct
      781 tccccctggg aactcctccc gatcttatcg agcgacattc gactggactc aagcttcaag
      841 ggtatttgct gggaataggg aggaattttt cccagaccat gaaccaacac acaatagcct
      901 tgataaagac ctcgaccgac ccggccgcgt aattgatgca attgcgccaa cccaaaccgt
      961 tcggcatgtt caatgagcat gaccgtggca ttgtgaacat ccaagccgac ctcgacaaca
     1021 gtggtggcca ccaacacatc aatctctttc tttttaaact gggccataac cacttgtttt
     1081 tcgcgggatg gcaatcggcc atgcagcaaa ccgatacgaa atggagcaca ttcttcccgt
     1141 aattgctcgg cagcctcaat ggctgcctgt aaatcaattt tttctgatgg ttcaactagc
     1201 ggatacacca cataagcctg ccgccccgct tctaattctt ttcgcatcaa acgatgcgcg
     1261 tctttccgtt gaccggtcgg gaacaacatc gtctttacgg gttttttccc tggaggaaac
     1321 tgatcaatca ccgacacatc caaatcccca tacagcgtca tcgccaaggt cctggggata
     1381 ggcgtggcgg tcatcaccaa cacatcggga tgccatggcg ctttcccacg aagctgcgcg
     1441 cgctgcagca caccgaattt gtgttgctca tccacaatca ccaacccaag cttggcaaag
     1501 tgcacatccg gctgtaacaa cgcgtgtgta cccaccacca cctgcacctg acctgactgc
     1561 attccggcaa ggatcttccc tcgctcgcgt ccggtctgcc cgcccttcaa caaaaggcac
     1621 cggacaccca gagcatcaaa taggggaagc aacgacagat aatgctgttc gctcaacact
     1681 tccgtgggag ccatcagggc cgcttgatat ccggacccac aggccatcac gatggcatga
     1741 agagccacca cggtcttccc gcacccgaca tctccttgca gcaagcggtt catactggtt
     1801 ggccgtccca tatcatggct aatttccttg atcacccgct cttgcgcgga agtgagcgaa
     1861 aagggcaaca cggttttcag ctgttcaacc aaccggttgc gaacagcaaa tgcgatgcct
     1921 tcaatgtcct gagcctgctc ttggcgccgc atggcaagcg ctaattgcag gagaaacaat
     1981 tcttcaaacg ccaggcgttg gtgctctggc gtcgcgtagg cattcaaggc ctccaccgaa
     2041 cgctcctgat tgggaaaatg taagacgggt aatgcatctg gaaggggtgg cactccgagt
     2101 tgtgctcgca tggttaaggg caacatctct tgtaactgat cggcataatg gtcaaaaatt
     2161 gatcgaataa ttcgtcgaat ctgcttggaa ctcaacccat tcgtctcatg atagacgggc
     2221 acaatccggc ccccagccat gtccggcaat tcgtcctcat ccaacaattc atactccggc
     2281 ccgcgcatgg tcatcggtga gcttccagaa gaattcgaca agactggacc ggtcaataac
     2341 agagatacac caggcacaaa ggttttctcc aaatagggct ggttaaaaaa cacgcattcg
     2401 ataagacccg tctcatccct caccgtcaca gtgaccacga ccagacgtct tcgataggtg
     2461 gttttcaccc acactttctg caccctgcct ttaatggtgg ccttcatgcc cgggagaaga
     2521 ttcccgatgg gcaacacttc caatcggttt tcgtatcgcc agggtaaaaa ccagaaagca
     2581 tcctctaatg tttgcactgc taatttttcc agcaagcgag ccctccgggg tcccacccct
     2641 ttggcaaatt gaatgggaat ctcagagaga gaggaatgag tcggcatgtt gattacaaaa
     2701 agcctattac atcagaaccg gaagacttac atcgaacgac tatcgaagag tctgcttaaa
     2761 ccggccccat ctgcaataat atggctcatg aggaatttga gggtgaaaaa caacatatct
     2821 aagtaaaaca cagactgcct gtgaaacgta cgaatgtgaa tgaattagtg tcaacagaaa
     2881 atggtcatgc gtcagtttgg cttgtcagcc ttgttatccc gcaatacagg ctagatagcc
     2941 gccaattgct caccccgaca gtacatttca cttaaccgat tgggttcatc cttcctaaaa
     3001 gggatttgtc gaaggccctc aattctcatt caagcacggc gctccaaacc aacggacagt
     3061 actttccttc caaattggca ccaacgccag cttagcgcct cgccaaggcc ttaggagttt
     3121 ggaacgatac gccttcgccg gatgacccgg agttgtttct ttcgcggtga aattatcaag
     3181 ttgagctttc tgcgctcggt tcgcttgaac ctcaaggaca aatcgcctgt ctttatctaa
     3241 accagaagcg ttcatgtggc atgacagaac aatggtataa tcgccctccc tttcgccgca
     3301 gcgttgcaat accggtactt ctataactgc cacaccgggc actattcccg atgctgtgca
     3361 ccgtaggagc gacttccagt gaccttctag tgcccaggcg gaacaacaac cctttcattg
     3421 tggaggcttc cgggaccaca gaaaattttc attatcgagc ccttgtgtgt acaatagcag
     3481 ctgtggtaac aaacagactc taatcgacaa ttctgtccat tttaaatatc cagggagaag
     3541 accatggcct tttcatgtga cgtgtgcgga aaaaaccgct tagtgggaaa taatgtgagt
     3601 cacgccaata atcgcacaaa acgtgtcttt cggccgaacc tgcgta
//
LOCUS       DATIAL010000402        11011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1580088, whole genome shotgun sequence.
ACCESSION   DATIAL010000402 DATIAL010000000
VERSION     DATIAL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580088"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VLA60_12460"
     CDS             complement(<1..547)
                     /locus_tag="VLA60_12460"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HSF10219.1"
                     /translation="MAKKQPVKTSPPSTRKSAKYADQTVEIGTGGEVHQTSGKTNPVL
                     TTQQGIPVADDQNSLKIGERGPSALEDFHFREKMFHFDHERIPERVVHARGFGAHGYF
                     ENYESLADVTKADLFQRPRDKTPAFVRFSTVAGNKGSFDLARDVRGFAVKLYTKEGNW
                     DLVGNNMPVFFIQDAMKFPDLV"
     gene            858..1424
                     /locus_tag="VLA60_12465"
     CDS             858..1424
                     /locus_tag="VLA60_12465"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSF10220.1"
                     /translation="MMMQIGQTITDGTYQAFHKEQIRSLTLSECQGKWLVLVFYPGDF
                     TFICPTELQELGELYPEFQKLGAEILTVSTDSVFVHKAWHDTSPAIKNIQFPMVADTT
                     GRLSREFGTYLEDEGVSLRGSFLIDPDGVLKVGEIHDNSIGRNGRELLRKLQAAVHVR
                     EGKGEVCPASWSPGDKTLKPGLDLVGKI"
     gene            complement(1554..3197)
                     /locus_tag="VLA60_12470"
     CDS             complement(1554..3197)
                     /locus_tag="VLA60_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSF10221.1"
                     /translation="MTTSRTGGWIVGLAAAVLTMGTTSMAAAAGFTAYNDLAWGTGQL
                     ETNITKFTSPNGGSGLPATGLLKDFATGLDTGVTVTVAGGQFVAGVNDLQGADPTTGD
                     AFTIFDGKVNGKGVLTYIDDVNNSLVLTFSGLNPGKTYNLTFYAHRNNNGWDRASLVT
                     LSGQDAFTNTSSVATDNPDTGTYPGGVLFTGPTSASTRLPADNDNGYVAQFSGIDPGS
                     DGTVLLTISFDGSVSEQYKGKYGSAVRLIESSGSAGQDLNGDGKADLVFRNTNGNAAV
                     WLLNGAAAPLSTVSLGGVGSAWEVAGVGDTDADGNADIIWRNTTTGAVAVWLMNGGTR
                     LSAGFPGTASTAWSIQGVGDLNGDGKADLVFRHTNGSAAVWLLNGTAPPSSTGSLGVV
                     SSAYQISGVGDTDADGNADIIWRNTTNGAVAVWLMNGVTRTSVGFPGTASTAWSIQGV
                     GDLNGDGKADLVFRHTNGSAAVWLLNGTAAPSSTGSLGVVSSVWRIRQVGDTDADGNA
                     DIIWRNTSSGAVAVWLMNGVTRTSVGFPGTASTAWEIQP"
     gene            complement(3523..4716)
                     /locus_tag="VLA60_12475"
     CDS             complement(3523..4716)
                     /locus_tag="VLA60_12475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HSF10222.1"
                     /translation="MRAVCWNGTHDVRIENVPDPEILNPRDAIVRVTLTAICGSDLHL
                     YDGYIPTMKAGDILGHEFMGEIVEVGAGVQNLQNGDRVVVPFNIACGRCFFCTGKMWS
                     SCDNSNPNAWMAEAAYGHSPAGLFGYSHMLGGYAGGQAEYVRVPFADTGPLKIHNGIK
                     DEEVLFLSDIFPTGYQAAENCGIQEGDTIAVWGCGPVGQFAIRSALMLGAERVFAIDH
                     FPQRLDLARRGHPGQFHEDDRIETINFDEDDVYDYLMNATGGRGPDACIDAVGLESHG
                     TTVDAIFDRIKVASLLATDRIHVLRQAARAVRKNGTISIPGVYGGFADKFPIGAIFGK
                     GITIRCGQTHVHNYMQPLFERIEREEIHPSFIISHRYNLDEAPDAYKNFRYHQNEFRK
                     VVLCP"
     gene            complement(4870..5649)
                     /locus_tag="VLA60_12480"
     CDS             complement(4870..5649)
                     /locus_tag="VLA60_12480"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HSF10223.1"
                     /translation="MKSTNIGALEQILSLGIGCGLIVHGLGQQPRRGILPLLFGSGLV
                     IHGLTSHSRLYEAIGIDELHGSHIRHPLNRTVHLRESITINRSQEDLYSFWRDFKNLP
                     RFMQNIVAVQELDERHSRWQARGPFNKNFHWEAEILEERQNELIKWRTIEIHSNLEHE
                     GVLSLRRAAGNRGTILSLDCRWTPPGGVFGATMAKLLPDDPARQVSEDLRRFRQLMEA
                     GEISRNQHTSADSNHSELVQKVREVGDELGITDPVVTDQRP"
     gene            complement(5810..7396)
                     /locus_tag="VLA60_12485"
     CDS             complement(5810..7396)
                     /locus_tag="VLA60_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSF10224.1"
                     /translation="MSDSLRWWQEGAIYQLLVPSFLDTNGDGYGDLQGITRRLEYLEW
                     LGVRAIWLSPIYPSPLADLGYDITDYQNIEPKFGSLHDFDEMVTEAHRRNLKIILDWV
                     PNHTSDKHPWFLDSRSNRESAKRNWYLWRDGKPDGSPPNNWISVFGGSVWQWDATTTQ
                     FYLHTFLDKQPDLNWRNPDVRAALFDTMRFWLERGVDGFRIDALDLLLKDDQFRDNPP
                     NPEYEDGQGPDSRLLPHHTRDQEGIHEIVATMRRVAEEFGDDKMLAGELYLPVPQATA
                     YYGNDTRELHLPLTMKLAWTPWTVEDLSRSVQSYIQDVPPHGWPSWIFSTHDCLRLAM
                     RLQGELTRVAAMLLLTLPGTPVLYYGEEIGMRGVPIPAEQAIDPQGRRTGRNRDPERT
                     PMQWNPDLHAGFSSREPWLPIAEDFQTANVSSQSGNPRSLLTLYRRLFEIRHDKSFFL
                     NERPEFLNVHNSLVAYLRKTHDGDHLVVLNFSGEPQTLDRDPSFQARVCLSTFLDKEG
                     EKIHGLLDLRGHEGLILSCD"
     gene            7605..9674
                     /locus_tag="VLA60_12490"
     CDS             7605..9674
                     /locus_tag="VLA60_12490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSF10225.1"
                     /translation="MTGTPDNNMITRRLIRHTTGDDWRNFLIAREWLVTNGLGGYASG
                     TLAGIATRRYHGLFIAALPAPLGRQMTLAHLSERVRLADGTLIKLSGEAHDLPDPYPH
                     GIDYLTEFRLEMGLPVWRYEIPGATIEKHILMPHQQNTVHIIYRLLHGNSVRLKLMPS
                     VHNRSHDAPVNTPLPGVFRLTEVESKYELSVLPDLPTLRMHLRGARQAFTVNRQTLPE
                     RMYPVEESRGYAESGTLWSPGYFRADLSQDHPVCLTASTESWEVVTALPVDEVWRLEH
                     ERRRRILAAAPEGARSGIGPELVLAADQFIIKPVGRIADSARASAEGDEIRTIIAGYH
                     WFTDWGRDTMISLEGLALMTGRQAEAGYTLRLFAHYLRDGLLPNLFPEGDQQGLYHTA
                     DATLWFFQAIGRYLETTGDMETLNVLLPHLQAIAEHHLNGTRFGIRVDPEDGLLRQGE
                     EGYQLTWMDAKVDGWVVTPRRGKAVEINALWYNALCLLGGWVRRVKGNTEAGQWEAHA
                     ERTRMSFNRRFWYEPGRHLFDVVDGEQGDDAACRPNQLLAISLTHPVLDCRYWDAVLN
                     IVQDRLLTPVGLRSLSADHPSYNPKYDGNLLARDAAYHQGTVWAWLIGPFIDAWLKVH
                     PEDVAGARQFLSGLKDHLNEGCMGSISEIFDAEAPFTPRGCIAQAWSVAEVLRCWVKT
                     TPDVHEA"
     gene            10036..10182
                     /locus_tag="VLA60_12495"
     CDS             10036..10182
                     /locus_tag="VLA60_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10226.1"
                     /translation="MVFSWVADAIYISGWGGEEWRLVAFYTLSGGVVGALLAPFPDIL
                     IFGR"
     gene            10209..10424
                     /locus_tag="VLA60_12500"
     CDS             10209..10424
                     /locus_tag="VLA60_12500"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HSF10227.1"
                     /translation="MWPMTLNLIIVALFGVNAWLRAADPTWEEGFILSTLGVLLLGIS
                     GWLGGELVYKHGVGVDTGHVLRTAEKR"
     gene            10738..>11011
                     /locus_tag="VLA60_12505"
     CDS             10738..>11011
                     /locus_tag="VLA60_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10228.1"
                     /translation="MNHLYVVFICISLTLIGCDEFPKDTHHTLQHIQSRGVLRIGAID
                     QAPWAYCEEGVAKGIEIKLAMAFARSLGVEPHIEFLSEADATEYLRQ"
BASE COUNT         2597 a         2941 c         2843 g         2630 t
ORIGIN      
        1 gaacgaggtc tggaaatttc atggcgtcct gaataaaaaa cacgggcata ttgttgccca
       61 caagatccca atttccctct ttggtataca gctttacagc aaatcccctg acgtctcttg
      121 ccaaatcaaa ggagccctta ttgccggcta cggtagaaaa gcggacgaac gccggagttt
      181 tatcacgtgg tcgttgaaag aggtcggctt tggtaacatc tgccagagat tcgtagtttt
      241 caaaatatcc atgagccccg aacccgcggg catgcacaac cctttctgga atccgctcat
      301 ggtcaaagtg aaacatcttc tcccgaaagt gaaagtcttc caatgcgctt ggaccccgct
      361 ctccgatttt cagcgagttt tggtcatccg cgacaggaat accctgctga gtcgttaata
      421 ccggattggt ctttccgctt gtctggtgga cttcccctcc tgtgccgatc tcgacagttt
      481 ggtctgcata tttggcggat ttccgggtgg aaggaggaga agttttaacg ggttgctttt
      541 tggccatgcg aatcgctcct tatcattaat gatttcgtga tgaataggtg agtaaacatc
      601 tgacgctacg ctatccatca tatagaacca attgcagagc ccatactgta agaaaccctg
      661 gtaagaaatt tcaggaactg ttatgaggta acaggagttc acgcaaggtt tcatggcagt
      721 cacaattttt tgcattccgg gataagattt gtttaagtaa cttgagaatg ggagttcaga
      781 tacacacgcc caaatatctg ctctgaaaca cttgtgctaa acattacaac ccgtttcgat
      841 aaaggaggtc gaaaagcatg atgatgcaaa ttggacaaac gatcacggat ggcacctacc
      901 aggctttcca caaagaacaa atccgttcac tgaccttgtc ggaatgtcag ggaaagtggt
      961 tggtgctggt gttttatccg ggagatttca cgtttatttg tccaaccgaa ttacaagaac
     1021 tgggagagtt gtatccggaa tttcaaaaac tcggggctga gatacttacc gtcagcaccg
     1081 attcggtctt tgtgcataag gcctggcacg atacctcgcc tgccataaaa aatatacagt
     1141 tcccgatggt agctgatacg actggccggc taagccggga gttcggaacc tatttggagg
     1201 atgaaggggt atcgttacgc gggagctttc ttatcgatcc ggatggtgtg ctaaaagttg
     1261 gagaaataca cgataatagc attggacgaa atggtcggga gctcttacga aagcttcagg
     1321 ctgctgtcca tgttagagaa ggaaaaggcg aagtatgtcc cgccagctgg tcccccggcg
     1381 ataagacgtt gaagccaggc ttggatctgg tcggaaaaat ttaatccaaa ggccatggtc
     1441 gatcaaccat ttcatctgga gtagggatcg gcgtcatcca gccaggtgac tctcgcttgt
     1501 gaaagaagcg agagtcacct acagcaggcc tcatattcga ccggtttatc aagctaaggt
     1561 tgaatttccc aggccgtcga cgccgtgccg gggaagccca cggaagtcct tgtcaccccg
     1621 ttcatcaacc acaccgccac cgcgccgctc gaggtattgc gccagataat atccgcgttg
     1681 ccatcggcat ccgtatcgcc gacttgtcta atccgccaga ccgagctcac cacacctaag
     1741 gagccggttg aagagggcgc tgcggtccca ttcagcaacc agaccgccgc gctgccattg
     1801 gtgtgtctaa agaccagatc agccttacca tccccgttca gatccccgac cccctggata
     1861 gaccatgcgg tcgacgccgt gccggggaag cctacagagg tccgggtcac cccattcatc
     1921 aaccacaccg ccaccgcgcc gttcgtggtg ttgcgccaaa taatatctgc attgccgtcg
     1981 gcatcagtat cccccacccc cgagatctga taagcggagc tcactacgcc taaggagccg
     2041 gtagaggagg ggggcgcggt cccattcagc aaccagaccg ccgcactgcc attcgtgtgt
     2101 ctaaagacca ggtcggcttt gccgtcccca ttcagatccc cgactccctg gatagaccat
     2161 gcggttgacg ccgtgccggg gaagcccgcc gaaagccggg ttcctccgtt catcaaccac
     2221 accgccaccg cgcccgtggt ggtattgcgc cagatgatat ccgcgttgcc atcggcatcg
     2281 gtatcgccta ctcctgctac ctcccaggcg gaacccaccc cgccgagaga gacggtggac
     2341 aagggagctg ctgccccatt caacagccag actgcggcat taccattcgt attcctaaag
     2401 acaaggtcag ctttgccatc tccattcaaa tcctgtccag ccgaccccga tgactcaatc
     2461 aaccgaaccg cgctgccgta ctttccctta tattgctcac tcacacttcc atcaaaactg
     2521 atagtcagca gcaccgtccc atcgctaccg ggatcaattc cgctaaactg ggctacatag
     2581 ccattatcat tgtccgctgg caatcttgtg gaagcacttg tcggtcccgt aaacagcaca
     2641 cctccaggat acgttcccgt atcaggatta tcagtcgcaa cggaactcgt attcgtaaag
     2701 gcgtcctgcc cggaaagggt caccagggaa gcccgatccc acccgttatt gtttcgatga
     2761 gcatagaagg tcagattata cgttttgcca gggtttagcc cgctaaatgt caacaccagg
     2821 gaattattca catcatcgat ataggtaagc acgccctttc cgttgacctt cccatcaaag
     2881 atcgtaaagg catcaccggt agtcgggtct gccccttgca gatcattgac cccggcaaca
     2941 aattggccgc cggccacggt cacggtcacg cccgtatcaa ggccggtagc aaaatctttc
     3001 agtaggccgg tggcgggaag gccagagccc ccattagggg aagtgaactt cgtgatattc
     3061 gtttccagtt gcccagtccc ccaagccaga tcattatagg cggtaaaacc agctgccgca
     3121 gccatagagg tcgtccccat ggtcaagacc gccgccgcga ggcccactat ccacccgcca
     3181 gtccgagacg tagtcatttt cataaatccc cctttccttt tccgccttga gttgaagcat
     3241 tatttgcatg aacactgtcc atttcacgat acctccttaa aaagtctacg cgggaacatg
     3301 gttgaagggg gtccggccaa gagccaggcc gccagcctat cttgagtcag gtggatgcgc
     3361 cgcttgcggg actcgccgcc ggtcttccgg gaaaaagaag agataggccg gtggaaaatg
     3421 tccactatcc ctcatcatct tcctcatctg tttttttgag gagccattca gggttgaaat
     3481 gaccgaacat tcttttttac tctgatttcg ttccgacaag cttcaagggc aaagcacaac
     3541 tttcctgaac tcattttggt gataccgaaa atttttatag gcgtcaggag cttcatcaag
     3601 attatagcgg tgtgagatga taaaagacgg atgaatctct tccctttcga tgcgttcaaa
     3661 caatggttgc atataattat gaacatgagt ctgaccacaa cgtatggtaa tgcctttacc
     3721 aaagatggcg ccaattggaa atttatcggc aaaaccccca tatacgccgg gtatggagat
     3781 cgtgccgttt ttgcgaacgg ctcgtgccgc ctggcgaagg acatgaatgc gatcggtcgc
     3841 gagaagactc gccaccttaa tccggtcgaa gatggcatca accgttgtgc cgtgggattc
     3901 gagccccacg gcgtcaatgc aggcatcagg gccccggcca ccggtcgcat tcatgagata
     3961 gtcatagacg tcatcctcat caaaattaat agtttctata cgatcatcct catgaaattg
     4021 tccggggtgg ccccggcgcg caaggtccag acgttgggga aaatgatcaa tggcaaatac
     4081 cctttccgct cctagcatca gggcgcttcg tatggcaaat tgccccacgg gtccacaacc
     4141 ccagacggca atggtatcgc cttcctgaat cccgcagttt tccgctgcct gatatcccgt
     4201 gggaaagata tcggagagga acaacacttc ctcatccttt ataccattgt gaattttgag
     4261 cggtcccgta tccgcaaaag gcacccggac atattcggct tgtcctcctg catacccgcc
     4321 aagcatgtgg gaatatccga acagtccggc aggggaatgt ccataggccg cctctgccat
     4381 ccatgcgttg gggttggaat tgtcgcagct cgaccacatc ttgccggtac aaaaaaaaca
     4441 ccttccacac gcgatgttaa atgggacgac gacccgatcg ccgttttgta gattttgtac
     4501 gccggctccg acttcgacaa tttctcccat aaactcatga ccgaggatgt cgccggcctt
     4561 catggtcgga atgtacccgt catacaggtg taggtctgag ccgcagatag ccgtaagggt
     4621 gacgcgaacg atcgcatcac ggggattgag aatttccggg tccggaacgt tctcgatccg
     4681 gacgtcatgt gttccgttcc aacagactgc tcgcatgaga agattccttc cagccttcaa
     4741 caggcattga cgtattgttc agaaagagac acggtgtagc tatcgacttt ttccggatcc
     4801 ggaattcccg tttcgttcaa gagtgaaatc gacaaataaa ggtcttacgt tcccaagttg
     4861 catcgccacc taggggcgtt ggtccgtaac cacggggtcg gtgatgccaa gttcgtcgcc
     4921 tacttcccgt accttctgca cgagctcgga atggttagaa tcggccgaag tatgctggtt
     4981 tcgcgaaatt tctccggcct ccattaattg gcgaaagcgg cgaagatctt cgctcacctg
     5041 acgcgcaggg tcatcgggaa ggagcttcgc cattgttgcg ccaaatactc ctcccggcgg
     5101 agtccatcga caatccaggg agagaatggt tccgcgattt ccggccgcgc gtcgcaggga
     5161 caagactccc tcgtgttcaa gattggaatg tatttcaatc gtccgccatt tgatcagttc
     5221 gttttgacgt tcttcaagta tctcggcttc ccaatgaaaa tttttattga aggggccacg
     5281 tgcctgccaa cgggaatgtc tctcgtcgag ttcctggacc gcgacgatat tttgcatgaa
     5341 ccgtggtaaa tttttaaaat cccgccaaaa cgaatagagg tcctcctgtg atcgattgat
     5401 cgtaatggac tctcgcaaat gcacggttct atttaaggga tgccgtatat gagacccatg
     5461 caattcgtca attccaatag cctcatacag gcggctatgg cttgtaagac catggatgac
     5521 taatccgctc ccgaataaca ggggtagaat accgcgtctg ggttgttgcc ctaagccatg
     5581 aacaattaag ccacagccga ttcctaagga aaggatttgc tccagagctc cgatattcgt
     5641 gcttttcata tgacatctcc aatataaatt atgccagccc ggagaacggg gcgattggca
     5701 atcaggccat cggaatcata ataccgatta agataccctc tcccttcctc cacggttact
     5761 ggtaatatcc ctggatataa accagaaacc gatcaatgaa aatatgtatt caatcgcatg
     5821 agaggatcag tccttcatgc ccgcgcagat cgagaaggcc atggattttc tcgccttcct
     5881 tgtcaagaaa cgtcgaaaga cagacgcggg cctgaaacga agggtcccga tcaagtgttt
     5941 gcggttcacc actaaagttg agtaccacca gatgatcacc gtcgtgagtc tttcgcaggt
     6001 aggccacaag cgagttgtga acattaagaa attccggtcg ctcgttcaaa aagaaagatt
     6061 tgtcgtgacg gatctcgaac agccggcgat acagagtgag gagtgagcgt ggattcccgc
     6121 tttgggagga cacgtttgcc gtctgaaaat cctctgcgat cgggagccag ggttctcgtg
     6181 aagagaatcc ggcgtggaga tccgggttcc attgcatcgg tgtgcgttcc ggatcgcggt
     6241 ttcgcccggt cctccggccc tgaggatcga tggcctgttc ggcaggaatc gggactccgc
     6301 gcatgccgat ctcttctcca tagtacagca ccggggtgcc gggcagcgtg agtaacaaca
     6361 tggctgccac gcgcgtcaat tctccttgga ggcgcatggc caaccgcaga caatcgtgcg
     6421 tgctgaaaat ccacgagggc catccgtgag gcggcacatc ctgaatatag gattgtaccg
     6481 agcgagacaa atcctcaacc gtccacggcg tccacgccag tttcatggtc aatggtaaat
     6541 gcaactcccg tgtatcgttt ccgtagtagg ctgtcgcttg aggcacgggc aaatacaatt
     6601 caccagccaa catcttgtcg tcaccgaact cttccgcgac cctgcgcatg gtggcaacaa
     6661 tttcatggat cccctcctga tcccgtgtgt gatgcggaag caatcggcta tccggtccct
     6721 gtccgtcctc gtactccgga tttggcgggt tatcgcgaaa ctgatcatcc ttaagcagca
     6781 aatccagagc gtcaatgcga aatccatcta ctcctctttc cagccagaac cgcatcgtgt
     6841 cgaatagggc cgctcggaca tccggattcc gccagttcag gtccggttgt ttgtcgagga
     6901 acgtatggag gtagaactgc gtcgtggtcg cgtcccattg ccagacgctg cccccaaaga
     6961 cactgatcca attattcgga ggggatccat ccggttttcc gtctcgccac agataccaat
     7021 tgcgcttggc actttctcga ttcgaccgtg aatcgagaaa ccagggatgc ttgtctgacg
     7081 tatgattggg cacccaatcc agtatgatct tgagattccg gcgatgggct tcggtcacca
     7141 tttcgtcaaa atcgtgcagc gatccaaatt tcggctcgat attttgatag tcggtgatat
     7201 cgtaaccaag gtcggccagg ggggatggat agatcggtga caaccaaatg gctcggactc
     7261 ccagccactc gaggtattcc aggcgccggg taattccctg cagatccccg tatccatctc
     7321 cgttggtatc aaggaaggaa gggactaaca gctgataaat ggcaccctcc tgccaccagc
     7381 gcagtgagtc tgacatgtcc gttcctcctt tcgtataagt cttatcagta ttccggttct
     7441 gtctcatgag agagggttct gtgagatgtt ggttataacc ggtgtcctac aataccaaga
     7501 gggcggccat ccactgtttg gctaatttgg aaaattttca ggtgaaaata tccgttgatg
     7561 cgagtgagaa attgtggttt taaaagaacc gacgacgaaa aaatatgacc ggaactcctg
     7621 acaacaatat gattacgcgg cgcctgatac gacacacaac cggcgacgat tggcgaaact
     7681 ttctgatcgc ccgggaatgg ctcgtgacca atggcttagg cgggtacgcc tcgggcacct
     7741 tggccggaat agccacccgc cgttaccacg gattatttat tgccgcactg cccgctcccc
     7801 ttgggaggca gatgaccttg gcacacctat cggaacgtgt ccgcctggca gatggcaccc
     7861 taatcaaact gagcggggag gcacatgacc tcccagatcc ctatccacac ggaatcgatt
     7921 atttgacgga atttcggctt gaaatggggc ttcccgtttg gcgttatgaa atacccggcg
     7981 cgacgatcga aaagcacatt ctcatgcctc atcaacaaaa taccgtgcat attatctatc
     8041 gactgcttca tgggaattcc gtccgattga aattgatgcc atcggtgcac aaccgttcgc
     8101 atgatgcacc ggtgaacaca cctttgcctg gcgtctttcg cttgacagaa gtcgagtcaa
     8161 aatatgaact gagtgtgttg ccggatcttc cgacgctccg aatgcacctg cgcggagctc
     8221 gacaggcatt taccgtgaat cgccagacct tgcccgaaag gatgtacccg gtagaagaga
     8281 gtcgcggata tgcagagagt ggaaccctct ggtcccccgg ttactttaga gccgatcttt
     8341 cacaggacca tcctgtgtgc ctcacggcgt cgaccgagtc ctgggaagtt gtgacggcct
     8401 tgccggtcga tgaggtttgg cggttggaac atgagcgacg gagacgcatc cttgcagcgg
     8461 cacccgaagg tgcccgcagc gggataggtc cggaactggt gctggccgca gaccaattta
     8521 ttattaagcc cgtcggacgt atcgcggatt ccgcgcgagc cagtgccgag ggagacgaaa
     8581 ttcgtacgat tatcgccggc tatcattggt ttactgattg gggtcgggac accatgatca
     8641 gtttggaagg tctggcattg atgacaggac gccaggccga agcagggtat accttacggc
     8701 tgtttgccca ttacctccgc gatgggctac tgccgaacct gtttcctgaa ggcgatcagc
     8761 aaggcctcta tcataccgcc gatgccacct tgtggttctt tcaagccatt ggccgttatc
     8821 tggaaaccac gggcgacatg gagacattga atgtgttact gcctcacctc caggcaatcg
     8881 ccgaacatca cctgaacggt acacggttcg ggattcgggt tgatccggaa gacggcttgt
     8941 tacggcaggg agaagagggc tatcaattaa catggatgga tgccaaagtc gacggttggg
     9001 tcgtcacccc gcgacgcggc aaagccgtcg agatcaatgc cctatggtat aacgccctat
     9061 gcctccttgg cggatgggtc cgccgggtta aaggaaacac tgaggccggg caatgggagg
     9121 cccacgctga gcgcacacga atgtctttta accggcgttt ttggtacgaa ccgggccgtc
     9181 acttattcga tgtggtggac ggtgagcaag gggacgatgc ggcctgtcgg cccaatcaat
     9241 tgctggccat ttccctgacg catcctgttc tcgattgccg atattgggac gcggttctga
     9301 atatcgttca ggaccgccta ctgacgcccg tcggattacg atctttgtcg gccgatcatc
     9361 ccagctacaa cccaaaatat gatggcaatt tgctcgcgcg cgacgcggct tatcatcaag
     9421 gaaccgtatg ggcatggctg atcggtccct tcatcgacgc gtggctcaaa gtgcatccag
     9481 aggatgtcgc aggggcgcgt caattcctat caggattgaa agaccatctg aacgaaggat
     9541 gcatgggcag catcagcgaa attttcgatg ctgaagcgcc ctttacccct cgcggctgta
     9601 ttgcgcaagc ctggagcgtg gcggaagttt tgcgctgttg ggttaaaaca actcccgacg
     9661 ttcatgaggc ataggacgta agtcctgcgg aggggtagtt ggaatgcctc ccactttcct
     9721 gcatcacctt tctctcatga ttccttctta ataattccac ggaaaacccc agacggaggc
     9781 gagccgaagt atcctgccgc caagtcactt tgccagggtt atatacagtt gtgtttccgc
     9841 tcgaattcaa aaatgatctt tcacagaaca cctgttggtc gtggaattct tccggaatca
     9901 ggattagggg tttaccgcat tctctgttac cacctggaga agaaacaaaa gagaaaggga
     9961 aagttctatg atgactcctg ccagtattaa agggcatcca ttgcacgtca tgttaattcc
    10021 cttgcctttc ggtgtatggt tttttcctgg gtggccgatg cgatctatat cagcgggtgg
    10081 ggaggagaag aatggcgcct cgtggccttc tatactctca gtggcggtgt ggtaggcgcc
    10141 ttactggctc cattcccgga tatcttgatt ttcggtcgct gagcgacccc cattctaaaa
    10201 aaattggcat ctggcctatg acccttaatt tgatcatcgt ggcattattt ggggtgaatg
    10261 cctggctacg tgcagcggac ccaacatggg aagagggatt tattctttcc acattgggag
    10321 tcctccttct gggaatatcc ggatggttgg gcggagaact ggtgtataag cacggcgtgg
    10381 gcgtcgacac gggacatgtc ctgcgcaccg cggaaaaacg ataacaccgg aacaatagga
    10441 gccaacaaat aaagccttgg acggtgtggc gatttttgat gtcggcccgg ttttggatgc
    10501 agctcaagga tactcatcat tttcaatcta tactttcaat gaccaccatt aagcagaaat
    10561 cttggtcctc gtgccttgga gagttccatg gacaggatga tctttcctct tatgtgtgcg
    10621 tatcattggt gcgtgtgttg tttgactcgt catgttttcc cttccgaaaa cggctactgt
    10681 tccaaccttc ctgcagacgg aaagcattgc atgtttctct gatatgaaga ggtcacgatg
    10741 aaccatttgt atgtcgtgtt catctgtatc tcactcactc ttatcgggtg tgacgagttc
    10801 cccaaggata cccaccatac acttcagcat attcagtccc ggggggtgtt acggataggt
    10861 gccattgacc aggccccgtg ggcctattgc gaagaagggg tggcgaaggg catcgaaatc
    10921 aagctggcca tggctttcgc ccggtcgctt ggcgtggaac ctcatattga gtttctctcc
    10981 gaggccgatg ccactgaata cctccggcag c
//
LOCUS       DATIAL010000403         5305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1256870, whole genome shotgun sequence.
ACCESSION   DATIAL010000403 DATIAL010000000
VERSION     DATIAL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256870"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1097)
                     /locus_tag="VLA60_12510"
     CDS             complement(108..1097)
                     /locus_tag="VLA60_12510"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSF10229.1"
                     /translation="MPEIQRIMLGPWKLLKSLFDTQEATALASHKNGREIDLVRTIPF
                     LALHIAVLAVFWVGWSPIAIAVAVSLYIIRMFAITGFYHRYFSHRTFRTSRVMQFLFA
                     CLGASAAQRGPLWWAANHRHHHAYSDKPADVHSPVIRGLWWSHVGWFLTHDQLATKLN
                     RIKDFARYPELRFLNRFDTLVPIGLAVILFLLGKWLGSAYPDLGTSGWQMLVWGFVIS
                     TVVLWHGTFTINSLSHLFGSRRYPTSDHSRNNFLLAIITLGEGWHNNHHQYCSSARQG
                     FRWWEIDVTYYLLEVLQLFGIIWDIRPVPAWVLNHPSLDQVDQDTDSAEKMAM"
     gene            complement(2171..2767)
                     /locus_tag="VLA60_12515"
     CDS             complement(2171..2767)
                     /locus_tag="VLA60_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSF10230.1"
                     /translation="MKQESGRPSKREQLIQTAVTLFGQNGIHATGIDTIVEHSGVTKK
                     TLYAHFRSKEELVLAALRHYDGQFRNSFMRQVETKARTPKTRLLAIFDVAETWFSQQS
                     FYGCLFINAVGEHSTPDTSLRYVCKDFKRMLTEFILKLCIQMGARNPQHLAEELSLLL
                     EGAIVTAQVSQKPDAAKIAKRVAAVLIEKQSPHETPQT"
     gene            3007..3468
                     /locus_tag="VLA60_12520"
     CDS             3007..3468
                     /locus_tag="VLA60_12520"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HSF10231.1"
                     /translation="MKMMKTILSSLFVMFLAIAATPAFAGDVTNSTPGISGYDPVAYF
                     TDGKPMRGSGYHVVEHKGVTYAFASKEHKEMFEADPGKYVPAYGGYCAYGVAVGKKFV
                     SDPEAWRIVQGKLYLNLDRDIQNKWSKDIPGYLKKSEANWKEIKDKAPSDL"
     gene            3549..4148
                     /locus_tag="VLA60_12525"
     CDS             3549..4148
                     /locus_tag="VLA60_12525"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HSF10232.1"
                     /translation="MKKFVTIGSVLILGLGSLAGWAMTDEGKPFAPNVDPKTGDISVP
                     ENYREWPTLGTWAHAKVEGAPGLQEYHVVYTQPDTIKYYKEKNRFPDGAVLVKELLNA
                     DTMPMTTGPAVGHATTIKGWFVLVRDTTGRFKKSSLWGDGWGGWSLFNPDDPQHTVSK
                     DYKIDCLPCHTPARELARKNAVEADKWIYSFGYPVLQRK"
     gene            4793..>5305
                     /locus_tag="VLA60_12530"
     CDS             4793..>5305
                     /locus_tag="VLA60_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HSF10233.1"
                     /translation="MKFLIYLVFLSFSWLGLTTQPLAAVGDVNYISDVVTVPLRSGPT
                     TAHRILHRGLPSGTQLTILAIDEEAGFTQVRTTDGMEGWVTSQYLIGEPIARVKLAAA
                     EKRLQGLKAEIEKEREARASIQAEHKETDANNRTLNLQVQSLSKELAELKRISGDSIN
                     EHARNIELVQQ"
BASE COUNT         1382 a         1237 c         1354 g         1332 t
ORIGIN      
        1 agatgtggga gagtgagaag gggaaggcct ggaggcgtaa atttaaaaaa ggcatgggtg
       61 aacaggccgg acaccttaaa agttcatcca tggggctaat agtgtatcta catcgccatc
      121 ttttcggctg aatcagtatc ctggtccacc tgatcaagac ttgggtgatt cagtacccaa
      181 gcggggactg gacgaatatc ccaaatgatc ccgaaaagtt gaagaacttc cagcaggtaa
      241 taggtgacat cgatttccca ccatcgaaat ccttgccggg cggagctgca atattgatga
      301 tgattattgt gccatccttc acccagggtg atgatggcca gaaggaagtt gttccggcta
      361 tggtcggagg ttggatagcg tcggctaccg aaaagatggg aaagggaatt gatagtgaag
      421 gtgccgtgcc agaggacaac ggtcgaaatc acgaagcccc acaccagcat ttgccatcca
      481 cttgtaccca gatccggata ggcactgccg agccatttcc ccaaaagaaa caggatgact
      541 gccagcccga ttggcacaag ggtgtcgaaa cgattcagaa atcgtagttc cggatatcgc
      601 gcaaaatctt tgatgcggtt caactttgtt gcgagttggt catgggttaa aaaccaacct
      661 acatggctcc accagagccc gcggatgacc ggagaatgga catctgccgg tttgtcggaa
      721 taggcatgat ggtggcgatg attagcggcc caccataaag ggcctcgttg ggccgcgcta
      781 gccccaaggc aggcaaatag gaactgcatc actcgtgagg tgcgaaaggt tcggtgggag
      841 aagtagcggt gataaaagcc ggtaatggcg aacattcgta taatatacaa ggacacggca
      901 accgcgatcg cgataggact ccagcccacc caaaagactg cgaggacggc aatatgtagg
      961 gccaaaaatg ggatcgttcg caccaggtct atctcgcgac catttttatg ggaagccagt
     1021 gctgtcgctt cttgggtgtc aaacagggac tttaacagtt tccatgggcc caacatgatc
     1081 ctctggattt ctggcatcct taacgctcct ctctcatgtg tggggaattc cgagatatct
     1141 atagcttacg attcacacaa gttcatcgcg gcctttttat aaaacggaga tcctcaactt
     1201 tcttattata aaggggaaag attcgaactc tacaaagtaa accagaagga caccttctgg
     1261 caaatacaat tttaccgggt ggatggcttt tggtctgtga tgggaggctg gatattctat
     1321 aagaatccgt tcaaaaaggc tgactcgcct gtccattatg gtgatactga aagtgaggaa
     1381 taatcatagt catacaaaaa aattcttcaa aactaaaatc tcatcatttt ttgctgattg
     1441 ggtgtagaag gggattggtt tcggggcttg gcatgttgtt atggatgtgg acggctacct
     1501 gggtttgggt gatagattaa gcgctccgat gtattggttc gatcctgata agttcttcga
     1561 agcaatggaa gaaacgcctg aatatttata ttgaaaggtg aaaggcaagc ccacgaaaga
     1621 tgcacccgct gttgttagca cctactgatt tatatgtatg tggatagcca aacaaaaaca
     1681 cccccgaaat taccactttg ttttccgtga cattttcatg ggggcagcta aggagattct
     1741 tattcattat ttccgcttga gtgtagcctg gaacttcttc cattccagga ggtcctggag
     1801 actgtggatg aggccaaaac gcaatcaaaa taggtcgctc atcgtgcggt atttccctga
     1861 atcacaagag gccaatccct gggtttctgt tgtagaagaa tctgcagaaa agtcaataaa
     1921 accaggatgg cctgcgctga aaccctctcg ctacatacgg atgcccactg aattttattt
     1981 cagtgggcga gaatggcatc ttagctccac ctgttagcct cccgggacct taaggctcat
     2041 ggcttctgat aaatggtgcg gcgtagcgat gcccaacgac caagcgccaa acttgtcaga
     2101 agttatattt gccgggtgat gatcatcaaa acaattaaga gtatccgata atccacctcg
     2161 aaatatgatg ctatgtttgg ggtgtttcat gtggactctg tttttcgatc agcactgctg
     2221 ctacccgttt ggcaattttg gctgcgtcag gtttttgtga cacttgggcg gtcacgattg
     2281 cgccttccaa taagagcgag agttcttcgg ctaaatgttg aggattccgg gctcccatct
     2341 gaatgcatag cttgaggata aactccgtca acatccgctt gaaatccttg cagacgtaac
     2401 gcagtgaagt gtcgggggta gaatgctcac caacggcatt gatgaacaga catccataaa
     2461 aactctgttg cgaaaaccag gtttcggcca catcaaaaat ggccaacaag cgagtttttg
     2521 gagtgcgcgc cttggtctcc acttgcctca tgaatgaatt tctaaactgt ccgtcatagt
     2581 gtcgcaatgc cgcgagcacc aattcctctt ttgagcggaa gtgcgcatac aaggtttttt
     2641 ttgtcacccc cgagtgttca acaatcgtat cgatccctgt ggcgtgaatc ccgttttgcc
     2701 caaataaggt cacggctgtc tggatgagtt gctcccgctt tgagggtcgc ccgctttctt
     2761 gcttcatact actagacctt caggtctacc gaaattttta caatttttgt ggatacataa
     2821 atgtctctga cagacatctg tcttttttgt ctttgtatcc caatggcttg aatggtttct
     2881 gcattgttaa cgcgactgga aaagaagtgt aacgaaacac gaactcagct gactaggctc
     2941 cataaaggat acagatctgt atacatccat ttactaacca ttctatcttc aaaaaggaga
     3001 tccatcatga aaatgatgaa gacaatctta agttccctgt tcgtcatgtt cctggccatc
     3061 gcggctacac ccgccttcgc gggggatgtc accaatagca ctccaggaat cagcggttat
     3121 gatccggttg cgtactttac ggatggcaaa ccgatgaggg gttccggtta ccacgtggtt
     3181 gagcacaagg gagtgaccta tgcctttgct tccaaagagc acaaagaaat gttcgaggcc
     3241 gaccccggga agtatgtgcc agcctacggc ggatattgtg cctatggtgt ggcggtcggg
     3301 aaaaaattcg tgtccgatcc tgaagcgtgg aggatcgttc agggcaaact gtatttgaac
     3361 ctggatcggg atatccaaaa caaatggtcc aaggatattc cgggctatct caaaaagtcg
     3421 gaggccaatt ggaaagaaat caaagataaa gcgccgtcgg atttataaag aaactaggcc
     3481 gaggtccgtc acggtttata tgacggacct cagccatttt tggatatcag tgttgtaagg
     3541 agaaatctat gaaaaagttt gtcaccatag gatcggtcct catcctggga ttggggagtt
     3601 tggcaggatg ggcgatgaca gatgagggga agccttttgc ccccaatgtt gatccaaaaa
     3661 ccggagacat cagtgttcct gaaaattacc gggaatggcc cacgcttggg acctgggccc
     3721 acgcaaaggt ggaaggtgcg ccaggtttgc aggaatacca cgtcgtgtat acccagccgg
     3781 acacgatcaa gtattacaag gagaaaaacc gattccccga cggagcggtg ttggtgaaag
     3841 aactgctaaa cgccgacacc atgccgatga ccaccgggcc ggcagtcggt cacgcgacca
     3901 ccattaaagg ctggtttgtg ttggtcaggg atacgaccgg gcggtttaaa aaatccagcc
     3961 tatggggtga tggttggggg ggttggtccc tctttaatcc ggatgatcct caacatacgg
     4021 tatctaagga ttacaaaatt gattgtcttc catgtcacac cccggccaga gagctggcgc
     4081 ggaaaaacgc ggttgaggcg gataaatgga tttactcatt tggttacccc gtgcttcaaa
     4141 ggaaatgaca gagcaactta aatatgaaga tccaattcat cggcactgtt cgaaaaagag
     4201 tttcactcgc ctgacatttg tccttttggc cacgacttat tatttgactt cggcttccga
     4261 gccgggttgg gttcgaaagc cgaggcttgt ttcattcggc caaaggacac aaagccattt
     4321 ctacccaagt ttgcagattg aatcgttctg acgcatacca tagaggggcg gaccaactcg
     4381 cctggctcaa ccagggtcct tcctgggttt gaatttcagc cccgttagct taatgacagg
     4441 cgggggtcat atgatcacct gagtcggcga ccaagtctgt ggttaagtgt ttcatttcat
     4501 cctaccctgc tcatccacct tctttaggcc cgtttgcgtg accatttccg ggtcttcttc
     4561 gtggacagaa aaggtgattc aatagtaagg caatacagaa gttaactttt ttaaggctca
     4621 attttcatga gccgattttt tggtgtcatt gacaccttac ggctcctttg ctacttatcg
     4681 gtttgtcccg cagggcttgt ttatttccta ttcccagtga gtacgacact ttacctttcg
     4741 tccgaagaac tcctttctcg aaatgcgcaa caccatgagg aggttaaacg ctttgaaatt
     4801 tttaatctat ctcgtctttt tatcgttctc ctggttgggg ttgaccacac agccattggc
     4861 tgcggtcgga gacgtgaatt acatctcgga cgtagtaacg gtgccgctac gcagtgggcc
     4921 cactactgcc catcgcatcc tacaccgtgg cctaccaagc gggacccaac tcaccattct
     4981 cgcgatagac gaggaggccg ggtttaccca ggtcaggacc accgatggga tggagggctg
     5041 ggtaacgtcc cagtacctta tcggggaacc catcgcccgg gtcaagctgg ctgcagccga
     5101 aaagcgccta caaggtctta aagctgaaat cgaaaaggag cgcgaagcgc gtgcgagcat
     5161 ccaggctgag cacaaagaga ccgacgccaa caaccggaca ctcaatttgc aggtgcaatc
     5221 cctctcgaaa gaactcgcgg aactgaagcg tatatcgggc gattccatca acgagcatgc
     5281 ccgtaacatc gaacttgttc agcag
//
LOCUS       DATIAL010000404         3337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1149058, whole genome shotgun sequence.
ACCESSION   DATIAL010000404 DATIAL010000000
VERSION     DATIAL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149058"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VLA60_12535"
     CDS             <1..547
                     /locus_tag="VLA60_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSF10234.1"
                     /translation="GTYDGKDHFAYFQHLSLGYTFMLPWAPRIMAMYDYASGTRNPHG
                     NESQTFDPLFGARRAELSPTSLFGPFYRSNISSPGIRLILEPLPVLRLDLKFRAWYLA
                     QSRDAWVNSGLQDSSGAAGNVLGQDVEVGVRWTPSPNLSIDAGFDHFFKGSYIKNQTN
                     VPGYPPAQDTNYFYVQTEVRF"
     gene            1377..>3337
                     /locus_tag="VLA60_12540"
     CDS             1377..>3337
                     /locus_tag="VLA60_12540"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HSF10235.1"
                     /translation="MNTRVLLLFMLSFSPLFLSMSQAQEQETPYNYPDVSPYAATVIG
                     TPPALKADLPKQIPVEDRELSVFPDRALPGILWYGENMRYSLVSQEKAAPLIFIIAGT
                     GAGYNSEKMQVLQRAFFQAGFHVLSLSSPTHPDFIVSASRTGIPGHLLEDSQDLYRVM
                     RLAWLEIQHEIKVTEFYLTGYSLGAAQAAFVSQLDEEQDDFHFTKVLLINPPVSLYTS
                     TSILDDMLANNIPGGTDKFPDFFDRVFHAFSEEYRHGEFVNFSGDFLYSAYKNLHLSD
                     ELLAALIGTSFRLSSSNMIFTADVLTNSGYIKPKNLILSATDSVTDYFKVSNRVTFLD
                     YFREMFYPFFQARYPGMTEQGLIANLSLATLEEYLRHNPKIGLLHNADDIILADGELD
                     YLKEVFGPRAKIYPRGGHCGNLDHRDTLSYIVDFFRNPLPANVARQPDRIFPTPTPVL
                     VGYQTHQATGMEDLPIPHIPVRISYTPSLDFQSRLDQQIQNLERKLQASHAYLQAAQN
                     RTIASENIPEPAHLVSALLEPDAKGVIIEPAKRPVEDLVHSDARYLVDVYDPIEAFNR
                     GTYKFNAQFDEYIFLPVVEGYEAVMPDFFEDRLSNFFSNIADIRNLFNAMLQLKGEAT
                     LNTLGRFLINCTFGLGGFFDHATPLGLIQ"
BASE COUNT          803 a          848 c          753 g          933 t
ORIGIN      
        1 aggcacgtat gatggcaagg atcattttgc ctatttccaa catctgtccc tgggctatac
       61 ctttatgctc ccctgggcac cccggatcat ggccatgtac gattacgcca gtggcaccag
      121 gaatccccat gggaacgaga gccagacctt tgatcctctg tttggcgcac ggcgagctga
      181 attatcgccc acgagtctgt ttggaccctt ctatcgctcc aatatcagtt cgccgggcat
      241 tcgattgatt ctcgagccgc ttcccgtcct tcgactcgac ctgaaattcc gggcctggta
      301 tctggcccaa tcccgggatg catgggtcaa ttcgggctta caggattcat ccggcgctgc
      361 agggaacgta ttgggacagg atgtggaggt aggcgtccgg tggactccct cgcccaatct
      421 cagcatagat gcgggttttg accacttttt caaaggttcc tatattaaaa accaaacgaa
      481 cgtgccaggg tatcccccgg cgcaggatac caattatttc tatgttcaaa ccgaagtcag
      541 gttttgatcc tgtgggaaga aaattttgcc aaaaaagaaa ttttttacgt ttgtgttttt
      601 cctacctaat taatattgca ggttattgaa gtacctactg ctgcttggcc taattcgtat
      661 tcgcttcaat cacaataatg acagcatcta cctaaaacgc aggtgtttgt tctcggcatc
      721 acgatggtga cattcacacc catgtgcttt acccgcagcg ccagactatt tgcgcgattg
      781 cccgtagagg ccgcaatggc agcaccggcc ccctgcttat cggtgaggtg tatcaaatta
      841 ttgtacgctc tgcgaatttt gaacaagcat tccgtttact catgcagtcg gaaacgagcg
      901 aagccacatc taggagtgta ttaagagcat aggtgcggac atcaagaatt cgcttcaggt
      961 aggactgtgg cataagtttt ccattgattt cagtcgatga cacagttcgc acaaatggaa
     1021 gaattccatc aatgcaatgc acaattggtt gcatacagga ccatttgatt ttgggacaca
     1081 cagtgacagt tcaatattca tgcactctcc ggtctatggt tcatttttgt ttacagtggt
     1141 tcctctcaat ttctattgta tgattccctt ttccaaaatc actccttctt tcaaaatgac
     1201 aggagcctga atcaggccgg tgctttaggc atgattggag gatggagatc ggcaggagag
     1261 ttgcacttta atattgaaaa tttccaacct attcagcaca atgtggaacc gaacttcatc
     1321 attttcctgg tgcttggatc aatttttcct cacacgcaaa attataaagg atgtgtatga
     1381 atacacgagt cttgcttctt ttcatgttat cgttttcccc cctcttttta tctatgagtc
     1441 aggcgcagga gcaagagacc ccctataatt atcctgatgt gagtccgtat gccgcaacgg
     1501 ttattggaac tccgccggcg ctgaaagccg accttcccaa gcagatcccg gttgaagatc
     1561 gtgaattgtc cgtctttcca gatcgcgcac tccccggcat tctctggtat ggggaaaata
     1621 tgcgttattc attggtctct caggaaaaag ccgcgccatt gatatttatc atcgccggaa
     1681 ccggcgcagg gtataattct gaaaagatgc aggttctgca gcgagcattt tttcaggccg
     1741 gtttccatgt tctctcctta tcctcgccta cccatccgga ctttatcgtc tcggcctcac
     1801 gcaccggaat ccccggtcac ctcctggagg attcacaaga cttatatcga gtcatgcgat
     1861 tggcctggct ggagattcaa cacgaaatta aggtcacaga gttttatctc acgggataca
     1921 gtttgggcgc agcacaggcc gcctttgtgt ctcagcttga cgaagaacag gacgatttcc
     1981 atttcacaaa agttctgctg atcaacccgc cggtaagctt atacacctcg acctccatcc
     2041 ttgatgacat gttggcgaac aatattcccg gcggcacgga taaatttccg gactttttcg
     2101 acagggtgtt tcatgccttt tcggaagaat accggcatgg agaattcgtg aattttagcg
     2161 gtgacttttt atattcagcc tataaaaacc ttcacctttc tgatgaactc ctggctgccc
     2221 tcattggcac ctcgtttcgc ctctcatcct ctaatatgat tttcactgct gacgtcttga
     2281 ccaattcggg ttatatcaaa ccgaaaaatc tgatcttgtc cgcgaccgac tcggtgacgg
     2341 actattttaa agtgtccaat cgtgtgacat ttttggatta ttttcgtgaa atgttttacc
     2401 cgttttttca agccagatat cccggcatga cggaacaggg gctcatcgca aacctcagct
     2461 tggccactct ggaggaatac ctcaggcaca acccgaaaat cggacttttg cataatgcgg
     2521 atgacatcat cctcgcagac ggcgagttgg attatctcaa ggaggtattt gggccgcggg
     2581 ccaaaattta tccgcgcggg ggacattgcg gcaatctgga ccaccgggac accctgagtt
     2641 atatcgtaga tttctttcgg aacccgcttc ccgccaacgt ggcccggcaa cctgatagga
     2701 tttttccgac gcccacaccg gttcttgttg ggtaccagac ccatcaggct accggaatgg
     2761 aagatcttcc tatccctcat attccagtga ggatttcgta tacaccctct ctcgactttc
     2821 aatcccgact cgatcaacaa atccagaact tagagcgcaa gcttcaagca tctcatgcct
     2881 atctccaagc cgcacaaaac cggacgatcg cgtcagagaa tattcctgaa cctgcgcatc
     2941 tggtcagtgc cctattggaa ccggacgcta aaggtgtaat cattgaaccg gctaaacggc
     3001 ccgtcgagga cttggtgcat tctgatgcca gatatctcgt ggatgtttat gatcctattg
     3061 aagcgtttaa ccgagggacg tataaattta atgcacagtt tgatgaatat atctttctgc
     3121 ctgtggtgga agggtatgaa gccgtcatgc cggatttttt cgaggatcgt ctctcaaatt
     3181 tcttctcgaa tattgccgac atccgtaatc tgttcaacgc gatgcttcag ctcaaggggg
     3241 aagcaacact gaacaccttg ggacgctttc tcatcaactg cactttcggc ttaggaggat
     3301 ttttcgatca tgccactccg ttaggattga ttcaaca
//
LOCUS       DATIAL010000405         5581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_181944, whole genome shotgun sequence.
ACCESSION   DATIAL010000405 DATIAL010000000
VERSION     DATIAL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181944"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..464
                     /locus_tag="VLA60_12545"
     CDS             63..464
                     /locus_tag="VLA60_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSF10236.1"
                     /translation="MVAAEIGYTQKAFELGLAALLMDMADIGGNVKDGCHIASMGGTW
                     MALTYGFGGLRDDDGILTFRPRRAPDQPAKLRFPLTYRGQILDVELGPDLATYSLWEG
                     DGLVIRHEEEEIRLSPTTPMVTRPIVKRVSS"
     gene            complement(822..2042)
                     /locus_tag="VLA60_12550"
     CDS             complement(822..2042)
                     /locus_tag="VLA60_12550"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HSF10237.1"
                     /translation="MNIILPGGTIGILGGGQLGRMLAMEGRRMGYRIGVLDPVQNCPA
                     AQVADFFVQSELTNTQRVIDFVSQVDVVTIETELVPWRLLADIETGQATRPSSSVLAL
                     IQDRLVQREFLQDHSFPQTPFASVKNHATLTTAAQHVTFPAILKKRRAGYDGKGQIRV
                     TGADHLNEAWRELKEVPSVLEAVAPFKMELSVVLARSLQGDIQLYPLAENAHRQNILH
                     TTRVPARVADTIRLRAEELAVSLSEALDYCGVMAVELFLLEDDTLLINEIAPRPHNSG
                     HFTFGACVTSQFEQHLRAICGLPLGDTSLMHPVVMVNLLGDLWRNGPPKWDRLLAHPQ
                     VRLHLYGKTHAAPGRKMGHFLWIADNPDQSYQQAECLLQSMMDADSQEKSFPISNPAN
                     EPFQKQSRPLETPA"
     gene            complement(2039..2539)
                     /gene="purE"
                     /locus_tag="VLA60_12555"
     CDS             complement(2039..2539)
                     /gene="purE"
                     /locus_tag="VLA60_12555"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HSF10238.1"
                     /translation="MNTKSDHSNPLVAIIMGSRSDWETMRHASVILTELAVPHECRIV
                     SAHRTPDFLFEYVGSAQSRGIHVLIAGAGGAAHLPGMAAAKTILPVLGVPVRSRALQG
                     FDSLLSIVQMPKGIPVATHAIGEDGAVNAALQAAAILAVSDHGIGQRLKAYYDQRHTP
                     ALEKLI"
     gene            complement(2750..3004)
                     /locus_tag="VLA60_12560"
     CDS             complement(2750..3004)
                     /locus_tag="VLA60_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10239.1"
                     /translation="MYANTKSLFQANALQRLCNWSDQMRIHFQSGDKSVDRACEPGPA
                     DEEYRCRFMGTKDIQIAWDRLPGTVQRQVVVIGGAEIMEL"
     gene            complement(3530..3874)
                     /locus_tag="VLA60_12565"
     CDS             complement(3530..3874)
                     /locus_tag="VLA60_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10240.1"
                     /translation="MLDANTKAMVLEALMDEYKARAFYRLVLNTFGEIGPFSNIVNAE
                     ETHARALELLCGHFEIPLPKDDWVEKLEPPSSILEACQAGVQDEHDNIAMYDRFLRQT
                     KVPEVLTLFQRL"
     gene            4363..5349
                     /locus_tag="VLA60_12570"
     CDS             4363..5349
                     /locus_tag="VLA60_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF10241.1"
                     /translation="MKDQSTVKGERWSVILAGGEGERTRPFIEQWLGYHKPKQYCSFV
                     GHRSMLQHTWDRADYLTHPEQKVTVIAQNHFREVVSQSKNRSCGQVIGQPRNCDTAAG
                     IFLPLTSIRAQNPHATVVLYPADHFVFPENRFMDTVEKAIQATTIFQDRIMVLGVRPT
                     SLELEYGWIEPGGPLGSSGGLCVRLVHAFLEKPNAMQGLAAQAKGALWNTFIMTAKVE
                     TFWRLGWKCMPEIMERFEQWEKVIGTPQESRTLREIYHDMPQRNFSSDLLGCAPESVG
                     VLELKNVLWSDWGRPERIADTIRAIGKTPAFGLECLRESRRARNENQLTEVW"
BASE COUNT         1373 a         1419 c         1462 g         1327 t
ORIGIN      
        1 ttttgactat tatgaccctc taaccacagg agattcttct ctctcatcat gcatccagag
       61 taattgtcgc ggcggagatt ggctatacgc aaaaggcatt tgaattaggt ttggccgccc
      121 ttttgatgga tatggctgat atcggtggaa acgttaagga tggttgccac atcgcctcga
      181 tgggagggac gtggatggcc ctgacctacg ggtttggcgg gttgcgggat gacgatggga
      241 ttcttacgtt tagacccaga cgcgccccgg accaaccggc aaagttgcga tttcctctga
      301 cctatcgcgg gcaaatcctg gatgtggaac tcggcccgga tttagccaca tattcattat
      361 gggagggcga tggtctggtg atccgacacg aagaggaaga gattcgtctt tcaccaacga
      421 ctcccatggt cacccgaccc atcgtcaagc gggtttcctc ctgaggcggg ttaaccgtcc
      481 gggttgtggc gctcattcta tgggaaaatt ccagagtttc ccaataatga tagctcagag
      541 gaataccacc cccatatggc caaatcctcc acacgagaca ggatcagggt ttaaaggcga
      601 atcggacgtg cggatggtaa gcggatgccc aaagttgtag gcgcgtaaca gataccgcat
      661 ccaactgaat cattatcaaa acacaaagag catgaacaga ttggcagggg aaaccaacga
      721 gaggatcaca accgtcatcg tttagtcttg gctgtttgaa tgccgcatca tgccttgagg
      781 tgtcgcccgc attagaaacc ctgagtgaaa aacggcggca gtcatgccgg agtctccaat
      841 ggcctggatt gcttttggaa aggctcattg gctggattgc tgataggaaa acttttctct
      901 tgagaatctg catccatcat gctctgaagg agacattcgg cttgttggta tgattgatct
      961 ggattgtccg ctatccaaag aaaatgcccc attttgcgtc ctggcgcggc gtgtgttttt
     1021 ccataaaggt gaagccggac ctgcggatga gccagaagtc gatcccactt gggcggtccg
     1081 tttcgccata gatctccgag gagattcacc atcacaacgg gatgcatgag agaggtgtcc
     1141 cccaatggta atccgcaaat ggcgcggaga tgttgttcga attgggacgt cacgcaggcc
     1201 ccaaacgtga aatgtccact gttatgaggc cggggagcga tctcattgat caacaaggta
     1261 tcgtcctcaa gaagaaacag ctccaccgcc atgactccgc agtagtcaag cgcttctgag
     1321 agggaaacgg caagctcctc cgcccgtaaa cggatcgtgt ccgccacccg ggccggaacg
     1381 cgtgtggtat gaagaatgtt ctgccgatgt gcattctcag ccaggggata gagttgaata
     1441 tctccctgaa gactgcgggc cagcacgacc gatagttcca tcttgaaggg cgcgacggct
     1501 tccaaaacag acgggacttc ctttaattct cgccaggctt cattcagatg gtccgcgccg
     1561 gtaacccgga tttggccttt accgtcatat cctgcacggc gcttctttag aatagccggg
     1621 aatgtcacgt gttgagccgc agtggtcaag gtggcgtgat ttttgaccga ggcaaaggga
     1681 gtttgtggaa agctgtgatc ttgaagaaat tcccgttgaa ccagccgatc ctggatgaga
     1741 gcgagcacgg aagaggacgg acgggtcgct tgacccgttt caatgtcggc aagaagcctc
     1801 caaggcacca gttcggtctc aatggtcacc acatcaacct gagaaacaaa gtctatcacg
     1861 cgttgtgtgt tcgtcagttc tgattgcaca aaaaaatccg caacctgggc ggccggacaa
     1921 ttctgaacgg gatccaacac gccaatccga taccccatcc ggcggccttc catggccagc
     1981 atgcgaccaa gttggcctcc gcccaatatc ccgatcgtcc caccaggaag gatgatattc
     2041 atatgagttt ctccagtgca ggggtgtgtc gttggtcata ataggccttg agtcgttggc
     2101 cgatgccgtg atcagatacg gcaagaatgg ctgctgcctg caatgcggca ttgaccgctc
     2161 catcctcgcc gatcgcatgg gtcgcaaccg gaatgccctt tggcatttgg acaatcgata
     2221 agagcgaatc aaagccttgc agggctcgag accgcaccgg cactccgaga accggaagaa
     2281 tcgtttttgc cgcggccatt cccggcaagt gtgcggctcc tcctgccccg gcaatgagca
     2341 cgtggatgcc acggctttgg gccgatccga catactcaaa gagaaaatcc ggtgtgcggt
     2401 gggctgacac aatccggcat tcatggggaa cggccaattc cgtgaggatc acacttgcgt
     2461 gccgcatggt ctcccaatca ctgcggctcc ccatgataat cgccacgagt ggattggaat
     2521 gatcagattt agtattcatc gattaggttg cctcccccaa aatattctcc ctttaagcat
     2581 ccaccccgaa agcacccggt tccgtgaaca gatcgtgagg cggcagtcca gggtaatgaa
     2641 aggtgcagtc tcctgatatg agcctgagcc tttttccccg gctccggctc tgtccgtatt
     2701 tgtcgcaagg ccctcttctc tgtccgccct actgtacccc gcttcaccat cataattcca
     2761 taatttctgc accgccgatc accaccactt gccgctgaac ggttccaggt aaccggtccc
     2821 aggcaatttg aatatctttt gtccccataa accggcatcg gtactcttca tcagcaggtc
     2881 ctggctcaca tgccctgtcc acgcttttgt cccccgattg gaaatgaatt cgcatttgat
     2941 cggaccaatt acacaatcgc tgcaaggcgt tagcctggaa tagggatttg gtgttggcgt
     3001 acatttcttc cctgctttgg cactatgccg gccgggaaga ctctagccaa ttccagccca
     3061 cacgttaaag cccggcctcc tgcccaggca atcagttttc ggaaccttat cccgatgaag
     3121 ggggaatgcg acgggatttt tggggcgcga ggatgggcgg aacgctgtct taatgtttga
     3181 aaaattctga gggatagtaa cgcaagccct ggaagaccat gtcatccaga gtaaaacgaa
     3241 gcatctcgca aggtcttgac gtttccaacg acttcatcgt tccttcgctg ttgaatcgaa
     3301 ataacaggct ggtgaggatt cggtttatca caaataattc atccctccct taatgggaaa
     3361 attgcagcat ctacacatga tgtggatcca gtttgccatc ctcctgactt cgatggcgac
     3421 cgggccgacc ggctcggaat cggccaagcg gcatcgctcc tcttccggac tgaccgcctc
     3481 gagcaacaca gcgctcaaat gctgggagat ggtgctgtcg tgagacagcc tacagtcgtt
     3541 ggaataatgt cagaacttcg ggcaccttcg tctgcctgag aaaccggtca tacatcgcga
     3601 tattgtcgtg ttcatcttga acgccggctt gacaggcctc gagtatagac gagggaggct
     3661 caagcttctc tacccagtca tctttgggta acggaatttc aaagtgcccg cacagaagtt
     3721 caagcgctcg cgcatgggtt tcctccgcgt ttacgatatt gctgaatggc cctatttcac
     3781 cgaatgtgtt gagcacgagg cgatagaacg cccgcgcttt gtattcatcc attagcgctt
     3841 ccagaaccat cgccttggtg ttggcatcaa gcatgtgaat cacctcccat cttttgacta
     3901 ggcggggaaa ctcaccatcg acacgcccaa ggcgtagtga tttcctccct tctacctcgg
     3961 tgcttaaagc atggcaacgc tttccgatat gcatgtccct agatgaacgt ccagacgatt
     4021 gttggaggaa gactcattcc acactttatg ttgggctgat ccttttcacg atttctatat
     4081 attccatatt ccacgaaaag aactgtagat atttgccacg tccgtgcaaa attttttgta
     4141 gccaaatagg tcaagggttt ggggatcgga aaatgatgcc tgacgtcaga agcccaatat
     4201 aaaagaaatt ttcataatgg taccgaactt gaagagaaag taaagaaata ccaatcaaga
     4261 gagcgaaact attagaagaa ctgaaagatc cttaccctgt ttgcgttcgc agaaccaaaa
     4321 gttttgagaa gagtttttgg gtttgctgaa aaaggggact ccatgaaaga ccaaagcacg
     4381 gtaaaggggg aacgctggtc agtgatcttg gctggaggag aaggggagcg cacgcgtccg
     4441 tttattgagc agtggctggg gtatcacaag cccaaacagt attgttcgtt tgtggggcat
     4501 cgttccatgt tgcaacatac gtgggatcgc gcggactacc tgacccatcc tgaacaaaag
     4561 gtgacggtca ttgctcagaa ccactttcga gaagtggtgt ctcagtcgaa aaatcgatcc
     4621 tgtggccagg tgattggaca acctcggaac tgtgatacgg ctgccgggat ttttcttccc
     4681 ttaacgtcta ttcgggctca gaatcctcat gcgacggtgg tgctgtatcc agcagaccat
     4741 tttgtattcc ccgaaaatcg atttatggac acggttgaaa aggctattca ggcgaccacc
     4801 atctttcaag atcgaatcat ggtcctcggt gtcaggccca cgtctcttga gctcgagtat
     4861 ggatggatcg aaccaggagg acccctggga tcaagtggcg gactttgcgt aagactggtg
     4921 catgcctttc tcgagaagcc aaacgctatg caggggctag ccgctcaagc caaaggagcc
     4981 ctttggaata cctttatcat gaccgccaaa gtggagactt tctggagatt gggatggaag
     5041 tgtatgcccg agattatgga acgatttgaa caatgggaaa aagttatagg cacacctcag
     5101 gaaagtcgga ctctacgtga aatctatcat gatatgccgc agcggaactt ttcatccgat
     5161 cttttgggtt gtgcccctga atcggttgga gtgcttgaac tcaaaaatgt cctgtggagc
     5221 gattggggtc gccccgaacg tatcgctgac actattcgcg ccataggaaa aacgccggct
     5281 tttggattgg aatgcctgag ggaatcgcgt cgtgcacgca atgagaatca gttgacagag
     5341 gtctggtgat gcgaacgagt caacaatcag ggagccgtga agacgcaacc catcccattt
     5401 ccctcgggct aaagaaaagc cgttgtctct aaaaagagag gggctaaacc atccagcgga
     5461 gatctatgcc actggatccc aaaatgaatt tcccataaca gatgttggaa atgacgagtg
     5521 atcaaacaaa aatcccaatt tctccatttg gggacaggga tgatgtttca cattcatcaa
     5581 g
//
LOCUS       DATIAL010000406         7894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_503859, whole genome shotgun sequence.
ACCESSION   DATIAL010000406 DATIAL010000000
VERSION     DATIAL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503859"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /gene="rseP"
                     /locus_tag="VLA60_12575"
     CDS             complement(<1..1060)
                     /gene="rseP"
                     /locus_tag="VLA60_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HSF10242.1"
                     /translation="MNNLLSFSPDSIFLFGQKLWWFLVVLGVLVTFHEYGHYLAARWV
                     GVRVLKFSIGFGPKLIGRKIGDTEYLLAAIPLGGYVKLYGEEVSEAVSPAEQRESFIH
                     QSLPHKTLIVAAGPGFNFILSYLIFTGMLALGSPLFVPSIDNITPVIEAITPDSPAET
                     AGLHIGDRVIRANEEDISTLGELYQQVGKAHGRPVTLDVIRGDTVKTLIITPTVQMVP
                     DRPDEPQYTLGIEDHAPLVGGVMPDTPAMAAGLQKDDRIIQINDTPIVTWSQMTEIVR
                     QHPGTPLEVQVDRGGQTVSLRITPEGQTVTSSDGETKSVGRIGIKLAGAGTVLKSTSL
                     FLAPWDGLKATWKWCELTI"
     gene            complement(1078..2247)
                     /locus_tag="VLA60_12580"
     CDS             complement(1078..2247)
                     /locus_tag="VLA60_12580"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HSF10243.1"
                     /translation="MKHIVILGSTGSIGNSTLDVISRFPEEFQVVGLAAGSNDQALEE
                     QIRIFKPEVAALSCQDAANRLRARMGRTSTEILDGEQGLCAVASASKGDLVISAIVGG
                     AGLKPTLTAIQAGRQVALANKEPMVMAGRLMQLEAHKHGVTIFPIDSEHSAIFQSMEG
                     HRKIDIRRIVLTASGGPFWDWPINDLEHVTPEQAMQHPNWKMGAKITTDSATLMNKGL
                     EVIEARWLFDIPHDQIDVVIHRESIIHSLVEYCDGSVIAQLGHPDMRTPISYALKYPE
                     RIPLNPPLLDLGKIGKFTFYPTDSKKFPCLQLAYDALAGADGLPPALNAANEIAVDAF
                     LKEQIAFLDIPRVIQETMNAYIATPVTSIEDALEVDQWARRTATEMMKACSSSPI"
     gene            complement(2284..3090)
                     /locus_tag="VLA60_12585"
     CDS             complement(2284..3090)
                     /locus_tag="VLA60_12585"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HSF10244.1"
                     /translation="MDPRRLYSAIVFIPLLYAGIRYSPPWLLSLLIGTASLLALWEFL
                     TLYFGPTGTLGSKIISCLGAVMLLVAMYTGYSQALNLWLLGIVMVVLTGFFIAPTAMR
                     QRLPLWTAYPFGILYVVVLLGHFILLRQLPHGIALIFFVLAITWLADTGGFVVGLSLG
                     RHALAPALSPKKTIEGLIGGVLFSVLGGLISHFWFLPFFSLGECAILGMGMAIIGALG
                     DLAESAIKRSVSVKDSGTIIPGHGGVLDRVDSLLFTGPAFYYYALFTGST"
     gene            complement(3171..3956)
                     /locus_tag="VLA60_12590"
     CDS             complement(3171..3956)
                     /locus_tag="VLA60_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HSF10245.1"
                     /translation="MDEFHAVEVRQVAEGDLLDQLDLTSLPRHIAIIMDGNGRWAAQR
                     GLPRIAGHKEGLRALQDVLEIGHELTIPYITIYAFSQENWKRPHSEIRLLMGLLEQYL
                     HQERQRFQERQVRFLPIGRLEQLPPSVRSLALEVAEETRHLTQRTLAVALSYGGRTEI
                     VDAVRKVAIEVQMGSLTPDQIDESLFEQYLSTWGLPDPDLLIRTSGEARISNFLPWQI
                     AYTELYFTKTLWPDFRRVETLLALLDYQKRERRFGRITQTVSP"
     gene            4184..5611
                     /gene="tldD"
                     /locus_tag="VLA60_12595"
     CDS             4184..5611
                     /gene="tldD"
                     /locus_tag="VLA60_12595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HSF10246.1"
                     /translation="MVPSLSDFALQEKDVLQALNKATARGVDYVDMYVESCVTDSVSM
                     EESLVKRAVKSISQGAGVRAVAGERTGFAYSDDLSVRDLEIAADTARYIADSPQGNNP
                     IAVTHRSQPSQNLYPLSQPLTDISTETRVDLLNKIDVEARRYDPRITKVMASFNTEQK
                     IFLVINSQGQLVGDVQPLSRLQITCIAEDGANRQIGSFGGGGRVGFAFYLEQNRAMEF
                     AREAARQAIVNLGAVEAPAGTMPVVLGGGWPGILLHEAIGHGLEADFNRRKTSAFSDL
                     VGKSVASELCTIVDDGTLPFRRGSMNIDDEGTPTSRTMLIEKGILRGYITDRLNARLM
                     GIPLTGNGRREDFRSIILPRMTNTFMLAGESDPQDIIRSVKNGLYAVSFGGGQVDITS
                     GKFVFSASEAYLIEDGKVTKPVKGATLIGNGPDVLKKVSMVGHDMKLDEGIGTCGKDG
                     QSVPVGVGLPTIKINEMIVGGTAMG"
     gene            5649..7019
                     /locus_tag="VLA60_12600"
     CDS             5649..7019
                     /locus_tag="VLA60_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HSF10247.1"
                     /translation="MSNLPITHGDRFPELAATVLKRAKALGATEADLLVAEGDSVSVQ
                     VRMSEVDRLSKAREKVLGIRVFFGKGSASSSTSDFSKASLDRLVSDTCSLARAVVEDE
                     TSGLPAPDQMVVDIPDMDMKDDRQLAVDEEIDLAKRTEQSAFSADPRITNSEGAECSA
                     GYGSILLANSHGFVGSYASSSYSLSVSPIALDSQNGGMQRDYWYSVKRKFHELDSPES
                     IGQEAARRTVRRLGSRAITTQEVPVIFDPETAKGLLGHISSAVSGYSLYKGASFLLGQ
                     LGKSIASDLVTVVDDGRLAGGLGSRPFDGEGLPTRRTLVIDRGVLSSYLLDTYSGRKL
                     GMASTGNASRSVGENPTVGATNLFLSPGSYSPDEILKSMKRGLYVTDLIGFGVNLVTG
                     DYSRGAVGFWVENGELTHPVEEVTIAGNLQQILKDIEMIGNDLEFRGRVASPTVKIRK
                     MMVAGH"
     gene            7113..7610
                     /locus_tag="VLA60_12605"
     CDS             7113..7610
                     /locus_tag="VLA60_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10248.1"
                     /translation="MPLEKEDGAKVLQLITSRYNAREWRKKIEKTLSLPPSGLKDEDQ
                     RKAFLYLKVGLQAYKSRRADPPSWIVGGFATKEVIDRALFKPTVIDPSFTKDQVTGFF
                     GEDPGSEVNEVWWEDMLVNWFEEPEEEVEDAIDGGSSEDSESLAEVDEASGPADAAHP
                     QKTIN"
BASE COUNT         1963 a         1824 c         2168 g         1939 t
ORIGIN      
        1 taatggtgag ttcacaccat ttccatgtgg ccttgagtcc atcccagggg gcgagaaata
       61 gagaggtgct tttgagcacc gtacccgcac cggctagttt aatcccaatg cggccaacag
      121 acttggtttc cccatcggaa gaggtaacgg tttggccttc aggggtgatg cgaagagaga
      181 cggtctgtcc tccgcgatcg acctgaactt ctaaaggggt tccagggtgt tggcgaacga
      241 tctcggtcat ttgagaccag gtgacaatcg gcgtgtcatt aatttggata attcggtcat
      301 ctttttgaag acccgcagcc atggcagggg tgtccggcat gactcccccg acaagtggag
      361 catgatcctc aatgcctaag gtgtattgag gctcgtccgg gcgatctggc accatttgaa
      421 ccgttggagt gatgatcagc gttttaacgg tatccccacg aatgacatcc agtgtgacgg
      481 gacgcccatg ggccttaccg acctgttggt atagttcacc cagagtcgag atgtcttctt
      541 cattggcccg gattacccga tcaccgatgt gtaatccggc agtttcggct ggggagtcgg
      601 gggtgatagc ttcaatgacc ggggtgatgt tgtcgatgct tggcacaaag aggggtgatc
      661 ccaaagccag cattcccgta aagatcaggt aactgagaat aaagttgaat ccggggccag
      721 ccgccacaat caacgttttg tgaggaagcg actggtgaat gaaggattca cgttgttcag
      781 caggtgaaac agcctctgac acttcctcac catataattt gacataaccg ccaagaggaa
      841 tcgctgccaa aagatattct gtatcgccaa tcttgcgacc aatgagtttt ggtccaaaac
      901 cgatggaaaa tttgaggacc ctgactccca cccatcgagc ggccagataa tggccgtatt
      961 cgtgaaaggt cacaaggact cccagtacga ccaggaacca ccataacttt tgcccaaata
     1021 gaaagatgga atctggagaa aatgacagga ggttattcat gggtgtttat cacgggttca
     1081 tattggtgaa gaggaacagg ctttcatcat ttctgtggct gtgcgtcggg cccattggtc
     1141 tacctccaag gcatcttcaa tggatgtgac gggagtcgcg atataggcat tcattgtttc
     1201 ctggatgact ctgggaatat ccaaaaaggc aatttgttcc ttcaggaagg catcaacggc
     1261 gatttcattt gcggcgttta atgcgggtgg aagtccgtcc gctcctgcca gtgcgtcata
     1321 ggctaactgg agacagggga attttttcga atcagttgga taaaacgtaa atttcccaat
     1381 ttttcctaaa tccaggagtg gaggattcaa aggaatgcgt tccgggtatt tgagagcata
     1441 ggaaattggt gttcgcatgt ccgggtgccc taattgcgca ataactgacc catcacaata
     1501 ttctaccaaa gagtggataa tactttctcg gtgaatgacg acatcaattt gatcgtgagg
     1561 aatatcaaag agccatcggg cttcaatcac ctccaggcct ttattcatca aggtagcgga
     1621 gtcggtggtg attttagcac ccattttcca atttggatgt tgcatggctt gttcgggggt
     1681 cacgtgttcc aggtcattga ttggccaatc ccaaaatggc ccgccggaag cggttaacac
     1741 gatccgccga atatctatct ttcggtggcc ttccatcgat tggaaaatgg cgctatgttc
     1801 actatcgatg ggaaaaatgg tgacgccatg tttgtgggct tccaattgca tcaaccggcc
     1861 tgccatgacc atcggctctt tattggcgag ggcaacctgc cggcctgcct gaatggctgt
     1921 taaggttggc ttgagtcctg ctccaccgac gatcgcagaa atcactaagt cacctttcga
     1981 tgcactggcg acggcgcaga gcccctgttc cccgtccaga atctccgttg acgtgcgtcc
     2041 catccttgct cgtaatcggt tggcggcatc ctggcaggag agcgcagcca cttccggctt
     2101 gaagatgcgg atctgttcct caagagcctg atcatttgat cctgcggcta agccgactac
     2161 ttgaaattct tcagggaatc tggagatcac atctaatgta ctgttcccaa tcgatccggt
     2221 agatcctaag atgacgatgt gtttcaccag attctctccc tatggttgtg cacggtctgg
     2281 ttattaggtt gagcccgtga atagggcata gtagtaaaag gccggtccgg taaagagcag
     2341 actgtccaca cgatccaaca ctcccccatg tcctggaatg attgtgcctg aatccttgac
     2401 actcacgcta cgtttgatcg cagattctgc aagatcccca agcgctccga ttatggccat
     2461 acccatgccg agaatagcgc attcccccaa cgagaaaaag gggagaaacc aaaagtggct
     2521 gataaggcct cccagcacag aaaaaagtac ccctccgatc aaaccttcaa tcgttttttt
     2581 aggactcagg gctggcgcca gggcatgtcg tccaagactt aaacctacga caaatccacc
     2641 ggtatcagct aaccatgtga tcgctagcac gaaaaagatt aaggcgattc catggggcag
     2701 ttgccggagg agtatgaaat gcccgagcag aaccacgaca taaaggattc caaagggata
     2761 ggccgtccat aaagggaggc gttgcctcat ggccgtcggg gctataaaaa aacccgttaa
     2821 aacaaccata acgattccca agagccagag gttcaaggct tgggagtagc cggtgtacat
     2881 ggcgaccagg agcatgacgg caccaagaca ggaaattatc ttgctgccaa gagtgccggt
     2941 tggtccaaag tagagcgtga gaaattccca taatgcgagg agagaagctg tgccaatcag
     3001 gagagaaagc agccaaggtg gagaatagcg tattcccgca tagagcaaag gaataaatac
     3061 gatggctgag tacagtcgac ggggatccat tgtttatagg agtaaaaggg gcagtgcgtc
     3121 agataaacaa gaagaagagt gccgggtatg caggagagcc acagacggtt ttatggcgaa
     3181 acagtttgag tgattcggcc aaaacgacgc tcccgttttt gataatcgag taaggcgaga
     3241 agagtctcaa cgcgacgaaa atcaggccac agggttttgg tgaaatagag ttcggtgtag
     3301 gcaatttgcc agggaaggaa attgctgatt cgcgcctctc cagatgttcg gatgagcaaa
     3361 tctggatccg gcagacccca tgtgctgaga tattgttcaa acaaggactc atcaatttgg
     3421 tctggtgtga gcgatcccat ctgaacctcg attgcgactt tccgcacggc atcgacaatc
     3481 tcggtacgtc ccccatagct gagggcgacc gccagcgtcc gctgagtcag atggcgggtt
     3541 tcctctgcca cctctaaggc cagcgaacgg acggaagggg gcagttgttc cagtcgtcca
     3601 attggcagaa atcgtacctg gcgctcttga aaccgttggc gttcttgatg caggtactgc
     3661 tcgagtaacc ccataagcag tcgaatttcg gaatgaggcc gtttccaatt ttcctgagaa
     3721 aatgcataga tggtgatata aggaatcgtg agttcatgcc caatttccaa aacatcttga
     3781 agggcccgga gaccttcttt gtgaccggca attcgaggca acccgcgttg agcggcccat
     3841 cggccattgc catccatgat gattgcgata tgtctgggga gggaggtgag gtcaagttgg
     3901 tccaacaaat cgccttcagc aacttgtctt acttccaccg cgtgaaattc atccatggaa
     3961 ttgtgctgaa cccgatcgta agggtaagaa aacggcgttg gaattctatt gaaagatttt
     4021 aggagctagc caaaatttca caataaattc agtctaataa tgaaagcgat tcttgtcaaa
     4081 tgaccgacga gacgatctta attggaaatt gcgcccacct gggagatggg ctatactcca
     4141 tttttgtttc tttgattacc tacttcgttg taattgatgt cctatggtac ccagcctttc
     4201 agattttgcc cttcaagaaa aggatgtcct ccaggcctta aataaggcca ctgctcgagg
     4261 agtggattac gtggacatgt atgtcgagtc ctgcgtgacc gattccgttt ctatggagga
     4321 aagcctcgtc aagcgcgcag tcaaaagtat ttcccagggg gcgggtgttc gagcggtggc
     4381 tggagaacga accgggtttg catattcaga tgatctatct gtgcgagacc tggaaattgc
     4441 cgctgatacg gcacggtata ttgccgattc tcctcaaggg aacaatccca tagccgtcac
     4501 tcatcgttct cagccctccc agaatctcta cccgttgtct cagccgctca cggatatttc
     4561 aacagaaacc cgggttgacc tcctgaataa aattgacgtg gaagccagac gatatgaccc
     4621 gcgaattacc aaggtcatgg cgtcttttaa tacggaacag aaaatattct tagtcatcaa
     4681 ctcccaaggg caactggtgg gagatgtgca gcctctttct cgtcttcaga ttacctgtat
     4741 tgccgaagat ggggccaatc gccagattgg gtcttttgga ggagggggac gagtcggatt
     4801 tgccttctat ctggagcaga accgcgcgat ggagtttgcc cgagaagctg cacgtcaggc
     4861 gattgtgaat ctaggcgcgg tcgaagctcc ggccggcacc atgccggttg tgttaggtgg
     4921 gggttggccg ggcattctgc ttcacgaagc tatcgggcat gggttggaag cggattttaa
     4981 tcgtcgcaaa acgtcagcct ttagtgattt agtgggcaaa tccgtcgcgt ccgaattatg
     5041 cacaattgtg gatgacggga cgctcccatt tcggcgtggg tcgatgaaca ttgatgatga
     5101 ggggacgccg acgtcacgaa ccatgctcat tgaaaaaggt attttgcgcg gctatatcac
     5161 ggatcggctc aatgcccgtt tgatgggtat cccgttaaca ggaaatggaa gacgggaaga
     5221 tttccggagt attatccttc cacgtatgac gaataccttt atgttagcgg gagaatccga
     5281 tccgcaggat atcattcggt cggtgaaaaa cgggttgtat gccgtgtcgt ttgggggagg
     5341 gcaggttgat atcaccagtg gaaaattcgt cttttcagcc agcgaagcct atctgatcga
     5401 agatgggaag gtgaccaaac cggttaaggg cgcgacgttg attgggaacg gtcctgatgt
     5461 gctcaagaaa gtttcgatgg ttggacatga tatgaaatta gatgaaggaa ttgggacctg
     5521 tggaaaagat ggacaatcgg ttccggtcgg cgtgggactt cctaccatta aaatcaatga
     5581 aatgatcgtc ggtggcacgg cgatgggctg atggataacc ctcccgtttt gtttctttcc
     5641 atttactaat gtcaaatttg cccatcacac atggtgaccg tttccccgag ttggccgcca
     5701 cggttctcaa acgagccaag gctttagggg cgacggaggc ggatctactt gtagcggaag
     5761 gcgattcagt ctcggtgcag gtgcggatgt cggaggtgga tcggctttcc aaagcccgtg
     5821 agaaggtctt ggggatccgg gtgttttttg gtaagggttc agctagttct tctacatcgg
     5881 atttttccaa agcctcgttg gatcgtttag tcagtgacac atgtagtttg gccagagctg
     5941 tggttgaaga tgaaacttca ggactgccag cgccggatca aatggtggtc gatatccccg
     6001 acatggatat gaaagatgat cggcaattgg cagttgatga agaaatagac ctggccaagc
     6061 gaaccgaaca atccgcgttt tccgcagatc cccgcatcac caattctgaa ggggctgagt
     6121 gttcggctgg ctatggatcg attttgttag cgaattccca tgggtttgtt ggatcgtacg
     6181 ccagttcttc ctactcactc tccgtgtcgc ccatcgcgtt ggacagccaa aatggcggta
     6241 tgcagcgaga ctattggtac tccgtgaagc gtaaattcca tgaattggat agcccggaat
     6301 cgatcggcca ggaggccgct cgccgaaccg tgaggcggct aggcagtcgg gccattacga
     6361 cacaagaagt tcccgtgatc ttcgatccgg aaacggccaa aggcctccta gggcatattt
     6421 cctcagcggt atccgggtac tccttgtata agggggcctc gtttcttttg ggacaactcg
     6481 gaaaatctat tgcttcagat ttggtgacag tggttgatga tggtcggtta gccggagggc
     6541 tggggtctcg accatttgat ggtgaagggt tgcccacgag aagaaccctg gtgatcgata
     6601 gaggcgtgct gtcgagttac ctgttagaca cgtattcagg acgaaaattg gggatggcat
     6661 ccacgggcaa tgcatcaaga tcggtgggcg aaaatccgac agtgggtgcc acgaatttat
     6721 ttctctcgcc aggctcctat tcaccagatg aaattctcaa gtcaatgaag cgagggctgt
     6781 acgtcacgga tctgattgga tttggtgtca accttgtcac gggagactat tcaaggggag
     6841 cggtggggtt ttgggttgaa aatggagagc tgacccatcc ggtggaagag gtcacgattg
     6901 ccgggaattt acaacagata ctgaaggata ttgaaatgat cgggaatgat ttagaatttc
     6961 gtggccgggt ggccagtcct accgttaaaa ttcgcaagat gatggttgcc ggtcattaga
     7021 ggtatacagg acaagaactc attcaagagt ctatcgatta cctatctatt tcatgaagtc
     7081 agaaagcggc ccgagggcca taaggagtca ggatgccact ggaaaaagag gatggggcaa
     7141 aagttctcca gttgatcact tcccggtaca atgcacggga atggagaaag aaaattgaaa
     7201 agactttaag tttgccccca tcagggttga aagacgaaga tcaacgcaaa gcgtttctgt
     7261 atcttaaggt cgggctgcaa gcctacaaat cccgccgggc ggatcctcct tcttggattg
     7321 tgggcggctt tgccaccaag gaagttattg accgggcatt gtttaaaccc acggtcattg
     7381 atccgagttt taccaaagac caggtgacag gattttttgg agaagatccc ggttcggaag
     7441 ttaatgaggt ctggtgggaa gacatgctcg tcaattggtt tgaagaacca gaagaagagg
     7501 tcgaagacgc catagacggc gggtcgtctg aagattcgga atcgttggct gaggtcgacg
     7561 aggcctcagg tcctgcagac gccgctcacc cccaaaaaac cattaactaa attcctttct
     7621 gtccggttcc ttcaggtccc ttgagcctaa ttttttgagt aatggcatgg gggatcatga
     7681 tggaaaccaa gctcctccgc ctttctcgtc ctcaccctta ccggttccat gccccttgca
     7741 ggaattcgtc catattcctt tcatcaattg aactcctggg tctgtcggct catgtatggg
     7801 gtgacggccg ccttgccttg cccggtttca gttcagggac cagcgttgat tgtgtgtgac
     7861 tatacctcga tgggggacat tggtcttatt ggcc
//
LOCUS       DATIAL010000407         8502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_291021, whole genome shotgun sequence.
ACCESSION   DATIAL010000407 DATIAL010000000
VERSION     DATIAL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291021"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VLA60_12610"
     CDS             <1..221
                     /locus_tag="VLA60_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10249.1"
                     /translation="LVLELKKTSNPLGSDCDRRRLNGFQAELGYSFGALVECQTSKGK
                     DPSIRVIEWTPDDGAPCYESFPAELGRE"
     gene            complement(864..2129)
                     /locus_tag="VLA60_12615"
     CDS             complement(864..2129)
                     /locus_tag="VLA60_12615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF10250.1"
                     /translation="MKFAKYPISDALKNNLSRLGFVKTTDIQFKAMPSIMAGEDVLAI
                     AQTGTGKTAAFAIPIINQIDQWKRSQRSEGIQCIVMVPTHELAMQIGEVFAQLSKQTK
                     VKAFAMYGGVEQDPQIQTLQNGIDVLIATPGRLFDLISQGYVLLDRVQTLVLDEADHM
                     LDLGFIGDIHAIKKKLPHKHQTLFFSATINAEIKKLAYSQVRHSAIRIQISPDNRVSK
                     NVSHYVMFVEMDDKRFFLERFLNDNPESRVIVFVRTRVRAERVAKAMGRVGIATLTIH
                     GEKDQRDRAAVMNAFKTGACRVLIATDVSARGIDIPDVHYVVNYDLPDKAENYVHRVG
                     RTGRGVKKGIAISFCSREEKAQLEEIQVFLDQEIEVVKITKKDYAFTVTHPSGELSLE
                     ELLAEIEEEEEEKRKKRKKIKPQKKKKKT"
     gene            complement(2656..5817)
                     /locus_tag="VLA60_12620"
     CDS             complement(2656..5817)
                     /locus_tag="VLA60_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HSF10251.1"
                     /translation="MVPNPSTSPSHRESGPLAWMVHNRVTPNLLMLTLLLGGLFMASH
                     IKQEVFPEFEEDTVTITVPFPGASPEEVEHGVILAIEEGVRGLEGVNEIRATAAENQG
                     TVVVELLAKSNNQKIYQDIQQAISRIVTFPEDTEKPRVTLDTRRRDVLTLQLYGDVSE
                     WTLREVAEQVRDRLLQEPGITQVDLEGARAYEIHVEVSQEDLRAYGLTLQGIAQTIAA
                     TALDRSGGSVETSSGEILLRVQERRNWASDFADIPIIANQTGTLLRLGDIAHVSEGFQ
                     ESETYATFNGVRAIGIEIFRVGDQTPIGVSESARAAMARIIPDLPDSIHYTILKDRSE
                     IYYQRLTLLLKNGFLGLLLVLAILTLFLEYKLAFWVTVGIPTSFLGAMLFLPAMDVSI
                     NMVSLFAFIIALGIVVDDAIIAGENIYEYRQRGKSFREAAILGARDIAIPISFSILTN
                     IVAFLPLMFMPGSFGKIWAVIPVVVSTAFIISWIEALFVLPAHLAHSGANPSGTIGRT
                     LHHTQQRFSAWFSRFVERTYGPFLRRAITHRYLTIAIGFGIFAIVLAYPFSGRMGFIL
                     MPTVEADFAKASAALPFGSPPEEMIRVRDGLIRSVQEVIDQYPGQHITNGTFALVLEN
                     TIDVRAYLPDANQRPLSTTELTTLWREHTPDFPGVEYLRFEADSGGPGRGAGISVELS
                     HRNIGMLDKASTDLADRLAEFGSVKDVDDGYSPGKPQLDFRIKEEARSLGLTAGDIAR
                     QARNAFYGSEALRQQRGRNEIKVLVRFPESERSSEYHVENLVIRTPAGPHVPLYQVAT
                     VEKGRAFREITRRDGHRTVTVTANVQPAKETNLVLTKLKSDVLPGLLRDYPGLSYSFE
                     GRQADMRDALQSFLYSSTLALILIYVLLAVPFRSYLQPAIVMTSIPFGLVGAVLGHMI
                     MGYNISIISMMGIIALGGVVVNDSLIMIDYANTRRREGHHACEAICQAGIRRFRPILL
                     TTLTTFGGLGPMIFETSRQARFMIPMAISLGFGILFATAIMLVLIPCLYLVAEDVLGV
                     FTVTVHTPSPKKVPNEPEVIVAE"
     gene            complement(5807..7030)
                     /locus_tag="VLA60_12625"
     CDS             complement(5807..7030)
                     /locus_tag="VLA60_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF10252.1"
                     /translation="MLPPSHSNIRLILVKAVLPVLILALAGGIAFSIVQTAPQPERHP
                     KPRQPRLVETVDINVTTQMLTIEAWGQVQPVHQITLRSQVNGKIISVNPELVPGGRFS
                     AQETILRLEPADYALAVRQRQSDLAKARVALLLEEGNQVVAKQEFDLLGATINDQEKA
                     LVLRLPQLRAMKADVDAAAAALQSAKLALARTVIKAPFEATVVRHHVDLGTYVTATTD
                     LVTIAGTKEYWVELAVPMAQLRWITIPRTPDEPGSAVKLFQENVWGKEKFRTGRVVRL
                     LTDLEPNGRMARLLVAIQDPLNLQAQHAPLPRVLLGSYLRAEIQGEELSRLAKVDRRW
                     IHDQDTVWLMNPDNTLEIRQVTIAYRGQHVVYISEGLETGDRLVVTDISAPTNGMPIR
                     LNTSRSDTPVTPHGT"
     gene            complement(7058..>8502)
                     /locus_tag="VLA60_12630"
     CDS             complement(7058..>8502)
                     /locus_tag="VLA60_12630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF10253.1"
                     /translation="PQHRMITLQYCRAILTGISLMLITGCLFDPQPIELKIQPPDSFS
                     LSGQQTLPDKWWEAFEDEALDRLIERALEDNPTLLATWDRLRQAEAIAVRDGAPRFPF
                     LNANSNASRSRTNTDIQTDRFAVGLSVAYELDLWGRVRAFGSAARFDAQASQADLTAA
                     AITLSSQMAKTWYNLLEQRGQLALLEKQISTNEKVLQLVTLRFRQGVAAAADVLRQKQ
                     LVEQTRGKTKDVRAQIATLTHQLAILLGKPPKSLEIPDNQALIRLPPLPSTGLPGELI
                     QRRPDVQAVFYQVQAADARVAAAIAERFPRIDLAGAITTNLTPTLTGGAFATAPGGLF
                     ANWLATVSAQIVAPIFNAGARAAQVDLNKAARSEVLHNYEFTVLQAFQEVENALIQEA
                     QQREKTSSLEEQFHIATQVIERLRARYLNGATAYLDVLNALNSQQELEREILTARRTL
                     IDFRVDLAKALAGGWSMTQPEIRQLTISKG"
BASE COUNT         1905 a         2249 c         2338 g         2010 t
ORIGIN      
        1 tgctggtgct ggaactcaag aagacgtcca acccactcgg tagtgattgc gatcggaggc
       61 gtctaaatgg cttccaagcc gaactcggct actcgtttgg cgctttggtc gagtgccaaa
      121 ccagcaaggg aaaggacccg agtattcgtg tgatcgagtg gacccccgat gatggagcac
      181 catgctatga aagtttccca gcagagttag gtcgcgaatg aagaataata agggtatacg
      241 ttgatctatg accaggccgt tccgatttga atttccacat gccctttatc accggacgtc
      301 acgtggagat cggcgggaag tgcctggggt catgtcttca gtatgacagc gggtagtcac
      361 gctttgtggg taatcgagta aagccgaaag tggattgcgt gattgcacga ccagacccca
      421 ttaaatactt cgggcccttt cctttaaccc gacgcctgct gttcggcctt aatggcggac
      481 gctcttcaag aattggcggg ccttgtttga gcctggcgag ttggcccgtc ctcctgtttc
      541 ctgcgtccgc ttaatctaaa gaggccgaac tgggcgtcaa tggttttggg tccttttgcc
      601 gaaacaaaag gacctcgact gccgggccga aacccggcaa ggtttaaaca attgaaagaa
      661 gaagctattg cttgtgtgca gtgaaaatcg gcttcgcagg cgaacggcag gggggtcatc
      721 ttttccgatt aggaaggggt tgaggccagt tcatgggctt tttggtattg gctcacgcgt
      781 atcttgaatt caagtccacc tgagcggaaa gcgagtcctc agtccaacaa agttgcaatt
      841 gattcgtatt cttaaacgag ccattaggtc ttttttttct tcttttgcgg tttaattttt
      901 ttcctttttt ttcttttctc ctcctcttcc tcctctattt cagctagtaa ctcttccaag
      961 ctgagttccc cgctgggatg tgtcaccgta aaggcataat cctttttcgt gattttgacg
     1021 acctcgatct cttgatctaa aaacacctga atctcctcaa gttgtgcctt ttcttctctg
     1081 ctgcagaacg aaatcgcaat cccctttttg acgccccgcc cggttcttcc cacacgatgc
     1141 acgtagtttt ctgccttatc cggcaggtca tagttcacca cataatggac atccggaatg
     1201 tcgatgccgc gtgcgctgac atctgtggca attaaaaccc gacaggcacc tgttttaaaa
     1261 gcgttcatga ccgctgcacg gtcacgctga tccttttccc catgaatggt cagcgtggct
     1321 attccgacac gacccatcgc cttggcaacc cgttcagcgc gcacccgcgt ccggacaaac
     1381 acgatcaccc ggctctcagg attatcgttc aggaaccgct ccaggaaaaa ccgtttatca
     1441 tccatttcaa cgaacatcac atagtgcgag acatttttgg agacacgatt gtccggggag
     1501 atttgaatcc ggatagccga atgcctcacc tgagaatagg ccagtttttt aatctcggca
     1561 ttaatggttg ccgagaaaaa gagggtttga tgtttgtgtg gcagcttttt tttgatcgca
     1621 tgaatatcac caataaatcc gagatcgagc atgtgatcag cttcatccag cacaagagtt
     1681 tggacccggt ccagcagaac atacccctga ctgatcaggt caaatagtcg accgggtgtc
     1741 gcaatgagca catcaatgcc attctgaagt gtttgtatct gcgggtcctg ctccacgccc
     1801 ccatacatgg caaacgcctt gaccttggtc tgcttggaaa gttgggcaaa gacctctcca
     1861 atctgcattg ccagttcgtg cgtcggcacc atgacaatac attggatccc ctcggagcgc
     1921 tgactccttt tccattggtc aatctgattg atgatcggga tggcaaatgc tgccgttttc
     1981 cccgttcctg tttgggcgat ggccagaaca tcttcccccg ccatgatcga gggcatggct
     2041 ttaaattgga tgtccgtggt ttttacaaac ccgagtcggg acaggttgtt ttttaaagca
     2101 tctgaaatgg gatattttgc aaatttcatg gtatttcaat tcctcttctt ctcatcgtat
     2161 acgacacaac aggggtggaa cattctggga atgctgaaaa aagccgccag aggcgttccc
     2221 tgccttcgcc ggagcgactt cgcgcaggca ggtcgccatt ttgccgtcct cacgtactaa
     2281 aagtacgctc ccggcaccaa catggctgcg gccttgctgg acgactgttt tgagcattcc
     2341 cgtcgaccat tgatatcgaa tccctgttga gtgaatacgt gcgttttttc agaaatattc
     2401 aaaaggaaca ttctgatttc ggactgtgcc acagtctact cggaaatgat cagacaggca
     2461 aaaaaacata agggagaaga aatgtgcttg gggtcatgtt tggggtcagc ttgccatgcg
     2521 ccatcggact gtcccctgag ggtaggtaag tgaagcccaa aggagatttc gtgatatcca
     2581 ggcatttcca catttttctt ttcagccatg tgctgatgaa gagtgtgctc tttagttatg
     2641 taaatagacc ttctcttact cggcaacgat gacttccggt tcattcggaa cttttttcgg
     2701 ggaaggcgtg tggaccgtaa cggtgaacac ccccaacaca tcctccgcga ccagatagag
     2761 acagggaatg agcacgagca tgatggcggt ggcaaagaga attccaaacc ccaatgaaat
     2821 ggccatggga atcatgaacc gggcctgacg ggacgtttcg aaaatcatgg gacccaaacc
     2881 cccgaaggtg gtgagggtgg ttaacagaat gggccggaac cgacggatgc ctgcctggca
     2941 gatggcctca cacgcatgat gtccttcccg ccgcctggta ttggcatagt cgatcatgat
     3001 taaggagtca ttgacgacca ctccgccaag cgcaatgatc cccatcatac tgatgatgct
     3061 gatgttgtag cccataatca tatggccgag caccgcacca accaatccga acggaatgga
     3121 tgtcatcaca atggccggtt gaaggtaact acgaaacggc acggcaagca acacataaat
     3181 caggatcaat gccagtgtcg aactgtataa aaaactctga agcgcatctc gcatatcggc
     3241 ctggcgtcct tcgaagctgt aactgaggcc gggataatca cgaaggaggc ccggaagcac
     3301 gtcgcttttc agtttagtca ggacaaggtt ggtttccttt gcgggttgga cattcgcggt
     3361 gacggtcacg gtccggtgtc catcccgtcg ggtgatttcc cgaaacgccc ggcctttttc
     3421 aactgtcgca acctgataca acggcacgtg tggaccggca ggcgttcgaa tcaccagatt
     3481 ttccacgtgg tattcgctcg aacgctccga ctccgggaat cgcaccagta ctttgatctc
     3541 gtttcgccct cgttgctggc gaagggcttc cgatccgtaa aacgcgttgc gggcttgtct
     3601 ggcgatatcc ccggcggtga ggccgaggct gcgggcctcc tctttgatgc ggaagtccag
     3661 ttggggtttc cccggagaat acccgtcgtc cacgtctttg acactgccga attcagccag
     3721 acggtcggcc aaatcggtgc ttgccttatc gagcatgcca atattccgat gactgagttc
     3781 aacactgatc ccggccccgc gccccggtcc cccgctatcc gcctcaaacc gcaggtattc
     3841 cactcccggg aaatccggag tgtgttcccg ccagagggtg gtcaattcgg tggtgctgag
     3901 cggacgttgg ttcgcatccg gcagataggc gcgaacgtcg atggtgtttt ccagcaccag
     3961 cgcaaaggtg ccgttggtaa tatgttgtcc cggatactgg tcgatcacct cttgcacgga
     4021 acggattaac ccgtcccgga cccggatcat ttcctcggga ggacttccga aaggcagcgc
     4081 ggctgaggcc ttggcaaaat cagcctcaac ggtgggcatg agaataaagc ccatgcgtcc
     4141 gctgaatgga taggccagca cgatggcaaa aatcccgaac ccgatggcaa tcgtcaggta
     4201 gcggtgagta atggcgcggc ggagaaaagg accataggtc cgttcgacga atcgggaaaa
     4261 ccatgcactg aaccgttgtt gggtgtgatg cagggttctg ccgatggtgc cgcttggatt
     4321 ggcgcccgaa tgcgccaaat gggccggaag aacgaacaag gcttcgatcc aggaaataat
     4381 gaaggcggtc gagaccacca cgggaatcac ggcccagatt tttccgaagg atccgggcat
     4441 gaacatcagc ggcaggaagg ccacgatatt cgtcaaaatg ctgaagctga tgggaatggc
     4501 aatatcccgt gcccccagaa tggccgcctc gcggaaggat ttgccgcgtt gccggtattc
     4561 ataaatgttt tctccggcaa taatggcgtc atccacgaca atcccgagcg caataataaa
     4621 tgcaaagagg gacaccatat taatagacac gtccatggcc ggcagaaaga gcatggcccc
     4681 cagaaatgac gtcgggatcc ccaccgttac ccagaacgcc agtttgtatt ccagaaacag
     4741 ggtgaggata gccaggacca gcaacaatcc gagaaatcca ttctttagca gaagcgtgag
     4801 ccgttgataa tagatttccg accggtcttt gagaatggtg taatggatgg agtccggcag
     4861 atcaggaata atacgggcca tcgcggcgcg tgcgctttcc gacacaccga tcggagtttg
     4921 atctcccact cgaaatattt cgatgccaat ggcacgtacc ccgttaaatg tggcatacgt
     4981 ttcgctttcc tggaatcctt ccgacacgtg ggcgatatct ccgagacgca ggagcgttcc
     5041 tgtttgattg gcaatgatgg gaatgtcggc aaagtctgac gcccaattac gtcgttcctg
     5101 tacccgcaac aggatttcgc cactggaggt ttccacgctt ccacccgatc gatcgagtgc
     5161 ggtcgcggca atggtttgtg caatgccctg cagggtgagc ccataggccc ggaggtcttc
     5221 ttgagacacc tccacgtgaa tttcataggc ccgcgcgcct tcaagatcga cttgcgtaat
     5281 gccgggttct tgtaaaagac ggtcccgaac ctgctcggcg acttcacgaa gcgtccattc
     5341 cgagacgtca ccatacaact gaagcgtcag gacgtcgcga cgacgggtgt ccaaggtgac
     5401 acgcggcttt tccgtatcct cgggaaaggt gacgatgcgg ctgatcgcct gctgaatgtc
     5461 ctgatagatt ttttgattgt tggacttggc caggagttcg acgacgactg ttccctgatt
     5521 ttccgcggcc gtggcccgga tctcgtttac gccctccaga ccacgcaccc cttcctcaat
     5581 cgccagtatc accccgtgtt cgacttcttc gggactcgcg cccggaaacg gaacggtgat
     5641 ggtgacggtg tcttcttcaa attcgggaaa gacctcctgc ttaatgtggg aggccataaa
     5701 cagtccaccc agaagtaacg tcaacatgag caaattgggc gtgacccggt tgtgcaccat
     5761 ccaggccaga gggccggatt cccggtgcga tggagacgtg gaaggattag gtaccatggg
     5821 gagtcaccgg tgtgtccgaa cggctggtat tcaggcgaat gggcattcca ttcgtggggg
     5881 cggaaatgtc ggtgaccact aagcggtctc ccgtttccag gccttcagaa atatagacca
     5941 catgttggcc acgataggcg atcgtcacct ggcgaatttc cagtgtattg tctggattca
     6001 tgagccacac ggtgtcctga tcatggatcc agcgacgatc caccttggcc agccggctga
     6061 gttcctcacc ctgaatttcc gcgcgaagat acgagccgag cagcacgcga ggcagcggcg
     6121 catgctgtgc ttgaagattc aaagggtctt gaatggccac cagcagtctg gccatacgcc
     6181 cgttgggttc aagatcggtc agcaaccgga ccacccggcc cgtccgaaat ttttcttttc
     6241 cccacacatt ttcctgaaag agtttgacag cggaccctgg ttcatcaggt gtgcgaggaa
     6301 tcgtgatcca ccgtaattga gccatgggaa cggctaactc gacccaatat tctttcgttc
     6361 ccgcaattgt cacgagatcg gtggtggcgg tcacataggt cccgagatca acgtgatggc
     6421 gaacgacggt tgcctcaaac ggggctttga tgacggtgcg tgccagcgcg agctttgcgg
     6481 attgcagggc agccgcggcg gcatccacgt cggctttcat cgcgcgcagt tgcggtaggc
     6541 gaaggaccag ggccttttcc tggtcgttga tcgttgcacc caataaatcg aactcttgtt
     6601 tggcgacgac ctggttgccc tcttcgagga ggagagccac gcgggccttc gctagatcgg
     6661 attgtcgttg gcggacagcc agcgcataat ccgccggttc caaacgaaga atggtttcct
     6721 gtgcgctgaa ccgaccgccg ggaacgagtt cagggttgac ggagatgatt ttcccgttca
     6781 cctgtgaccg gagtgtgatt tgatgaacag gctggacttg tccccaagcc tcgatggtca
     6841 gcatctgtgt ggtcacattg atgtcaacgg tttccaccag tcggggctgc ctgggtttgg
     6901 gatgccgttc aggttgtggg gcggtctgga caatcgaaaa cgcaatgcct ccagccagcg
     6961 ccaggatcaa aacaggcagg accgccttca ccaggatcag tcgtatgttg gaatgggatg
     7021 ggggtaacat gagttgggcg tttttcggga ggaatcccta tcccttggaa atggttaatt
     7081 gacggatttc aggttgagtc atggaccacc caccggccag agccttggcc agatcaaccc
     7141 gaaaatctat gagcgtacgt ctggcggtca ggatctcgcg ttcaagttcc tgttgactgt
     7201 tcagcgcatt aagcacgtcc agataggcgg ttgcaccatt cagataccgg gctcgcaagc
     7261 gttcgatcac ctgggtggcg atgtggaact gctcttccag actggaagtt ttttcccgtt
     7321 gttgggcttc ttgaatcaag gcattttcga cctcctggaa tgcctgaagt accgtgaatt
     7381 cgtagttgtg caggacttca gaccgagcgg ctttgttcag atcgacttgc gccgccctcg
     7441 cccccgcatt aaagatcggt gcaacgattt gtgccgatac ggtggctaac caattggcaa
     7501 aaagccctcc aggagcagtc gcaaaggctc cgccggtcag ggttggggtg agattggtgg
     7561 ttatcgcccc ggcaagatca atgcggggga aacgttcggc aatggcggcc gcgacacgcg
     7621 catcggctgc ctgtacctgg taaaacacgg cctgcacgtc agggcgccgt tgaatgagct
     7681 ccccgggcag gccggtactc ggcaatgggg gaaggcgtat cagcgcctga ttgtcgggga
     7741 tctccagaga cttgggaggt ttcccaagaa gaatggcaag ttgatgggtc agcgttgcga
     7801 tttgtgcgcg gacgtctttg gtttttccac gcgtttgttc aactaattgt ttctgtcgca
     7861 ggacgtcggc cgccgcggcc acgccctgtc ggaaccgcaa ggtgaccaat tgaagaactt
     7921 tctcgttggt cgatatctgt ttttccagca aagccagttg gcctcgttgt tcgagcagat
     7981 tataccaggt tttcgccatc tgactcgata acgtaatcgc ggcggccgtt aaatcggcct
     8041 gactggcctg cgcgtcgaag cgcgccgcac ttccgaaagc ccgtacccgc ccccacaggt
     8101 ccaactcgta ggccaccgat aacccaaccg caaaacgatc ggtctgaata tcggtattag
     8161 tgcgactccg gctggcatta ctgttcgcat ttaaaaatgg aaagcggggg gctccatcac
     8221 gcaccgcaat ggcttccgcc tgtcgcaggc ggtcccaggt ggccaacaga gtcgggttgt
     8281 cttccagcgc ccgttcaatc aaccggtcaa gggcttcgtc ttcaaaggct tcccaccatt
     8341 tgtccggcaa cgtttgctgg ccggaaaggg aaaaggaatc gggcggctga attttcaatt
     8401 caatgggttg tgggtcgaac aggcacccgg taatcagcat cagggaaatt cctgtgagga
     8461 tggcgcgaca atattggagg gtaatcatac gatgttgcgg at
//
LOCUS       DATIAL010000408         6648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1364281, whole genome shotgun sequence.
ACCESSION   DATIAL010000408 DATIAL010000000
VERSION     DATIAL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364281"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /gene="argH"
                     /locus_tag="VLA60_12635"
     CDS             <1..1156
                     /gene="argH"
                     /locus_tag="VLA60_12635"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSF10254.1"
                     /translation="VHMSIERRLTELIGPVGGKLHTGRSRNDQVTLDLRLYLRDTLQH
                     LSNDLRLLQQSLVTLAERYMGVMMPGYTHLQRAQPVLFSHHALAYVEMVERDKGRLHD
                     ALVRINVMPLGSGALAGNNYPIDRHYTASLLHFPRVTQNSLDAVSDRDYVIEVLSACA
                     IVMMHLSRLSEELILWSSQEFHFTDLPDGFCTGSSMMPQKKNPDVPELIRGKTGRVYG
                     HLFSLLTTLKGLPLSYNRDLQEDKEPLFDTLDTVTISVNIYAELLAQLTIRPEPMKEA
                     VSQGFLLATELADYLVKKGMPFRESHHVVGSLVRECLAKGKDLGQLTQQDLLNASSSF
                     DAKAFQVLTPEAAINSKNIIGGTATAQVTKQIKGWKKSLQAEMKHSMKRQ"
     gene            1162..1410
                     /locus_tag="VLA60_12640"
     CDS             1162..1410
                     /locus_tag="VLA60_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10255.1"
                     /translation="MRQILKIPTGYFLIFCILGLLSSSGCGAVGPPIPPEKVGIEAKR
                     LKQQQDQAKTEGAFDQDPLNVPLEEAVELPTVYPIDTR"
     gene            1495..3372
                     /locus_tag="VLA60_12645"
     CDS             1495..3372
                     /locus_tag="VLA60_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF10256.1"
                     /translation="MDTSLTQGAIRPKLLLLDPYPRNNPYRMTASERRSIWFPKLSLP
                     AIAAYTPTTWDVEIVDEAVRDIDFETPCDVVGISIMTCYAPRAYEIADEFRRRGKPVI
                     LGGVHPTYCPDEALRHCDAIVCGEAEDLWPQVITDIEAGSLKRIYRMDQFPTLSHYKP
                     PRIELLSPDSYMTRLCTFTTRGCHFDCEFCSVSPFNGKTTRRRPVPEVIEELKRAKEW
                     LRSDIIERMTRGSLFHALTTSLKIWVGLEEGSIVAFVDDLHNSHRAYCRELWQALKSL
                     NIKWGCQSTLFLGDDPEMVRLAADSGCVSVFVGMESLSDDSLDETNKGFNQVRKFENQ
                     IKMFHDHGIMVNPGLVFGFDNDDESVFETAVEFLTRNKVELAYFNVLTPLPGTALFER
                     FKREGRIIDQDWSKYDGKHVVYTPKRMSAEQLQEGFFWANHQFYSWPCIWKRMGHKSQ
                     RLIPRLEMNWEFRKLIYRTTPKGALSPLATVLKNLQTKLPTCETQQLVPNALHPQPSP
                     DSNHQELCLKMKARRHDAFAALFIDLEGTLDHLNAKELLKRITQAGREAKMDIVVNFE
                     HIKHATPKAIYTLLDGEILRAITPHTKLRYRNLKEAFQSIVSEIALENLDLFDEDIQH
                     A"
     gene            3365..4627
                     /gene="lysA"
                     /locus_tag="VLA60_12650"
     CDS             3365..4627
                     /gene="lysA"
                     /locus_tag="VLA60_12650"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSF10257.1"
                     /translation="MHDFHYQGDGLFCEEVPIRQITEQVGTPCYIYSHRTLIRHFHAF
                     DQAFNAIPHIVAFAMKANSNLTVLRLLAKEGCGADIVSGGELFRALTAGMAPNKIVFA
                     GVGKSKEEIQYALESDILMFNVESPGELQQINEVAGSMGLRAKVALRINPDIDPQTHP
                     YISTGLKKSKFGIGADRALEEFDAAGNLPHIEVVGVHSHIGSQLTQVTPFVDALKKAV
                     ALIQTLQAKGVHIQYLNIGGGLGITYSDETPPHPKELAEAISPLLQSVSCQIIMEPGR
                     SIVGNAGILVTKVLYNKASADKHFVIVDAAMNDLLRPSLYDAHHDIQPVLKKESSAVN
                     TVDVVGPICESGDFLAKDRKMPHSQPGDLLAVMSAGAYGFTMASNYNSRPRVPEVLVK
                     GKDITVIRKRESYEDLIRGETISEAFSN"
     gene            4729..5601
                     /gene="dapA"
                     /locus_tag="VLA60_12655"
     CDS             4729..5601
                     /gene="dapA"
                     /locus_tag="VLA60_12655"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HSF10258.1"
                     /translation="MFSGSLVAIVTPFSKGKFDEKAMADLIESQISSGTHGLVPCGTT
                     GESATLTPEEHERVVSFTVEVVNKRVPVIAGTGSNSTDEAITFTKHAKAVGADGALLI
                     TPYYNKPTQEGLYRHFAAIAKAVDLPQILYNIPGRTSINMLPTTVSRLSEIQNIIGIK
                     EGSGSLQQVSEIIQQSRPGFLVLSGDDPLTLPMIALGGKGVITVTANVAPTNMANMVN
                     AALKGDYETARLIHYKLSPLFGALFLETNPIPVKAALAMMGKMSEEVRLPLTPLAAEF
                     RPALQEALQQAGVL"
     gene            5615..6418
                     /gene="dapB"
                     /locus_tag="VLA60_12660"
     CDS             5615..6418
                     /gene="dapB"
                     /locus_tag="VLA60_12660"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HSF10259.1"
                     /translation="MIRTIITGAAGRMGMRLVALTQDTPGLKLSGAVEVKGHPSIGKD
                     AGEVAQIGQVNIPITDDLHVCLSQGDVVIDFTAPSSCLANIQHVVKSTKAMVIGTTGF
                     TDQELAQLKTFALKIPCVFSPNMSVGINVLLSTIGKIARSLGEQYNIEVIEAHHNKKK
                     DAPSGTALKLAEALAEGMAWDLQEVGVYTRHGMTGERKTREIGMQTIRAGDIVGDHTI
                     LLGGPGERIEITHRAHTRDTFAQGALRAAEWVAQKPPGLYSMADVLGLS"
BASE COUNT         1792 a         1704 c         1548 g         1604 t
ORIGIN      
        1 tgtgcacatg agcattgaac ggcgcctgac tgagctcatt gggcccgttg gcgggaaact
       61 ccacaccggc cgaagtcgga atgatcaagt caccctggat cttcggctct acctcaggga
      121 tactctccaa cacctgagca atgatctccg tttacttcaa cagtcgctcg tgacgctcgc
      181 tgagaggtat atgggggtga tgatgccggg ctatacacat ctacagaggg cccagcccgt
      241 cctcttttcg catcatgccc tagcttatgt tgaaatggtt gagcgggaca aagggcgtct
      301 ccatgatgcc ttggttcgaa ttaacgtcat gccgttaggt tccggtgcgt tagctggaaa
      361 caattacccg atcgatcgac actataccgc atccttatta catttccctc gcgtgaccca
      421 gaatagcctg gatgccgtgt ctgaccgaga ttatgtcatt gaggtgctta gcgcttgcgc
      481 catcgtgatg atgcacctct ctcgtttaag tgaggagctg attttatggt catcacaaga
      541 attccacttc acagatcttc ccgacggatt ttgtacaggc agcagcatga tgccacagaa
      601 aaaaaaccca gacgtccctg aactgatccg cggaaaaacc ggtcgggtct atggacattt
      661 gttcagcctc ctgaccactt taaaaggatt gcctctcagt tacaatcgag accttcagga
      721 agacaaagaa ccgttgtttg acacattgga taccgtgacg atatcggtta atatttacgc
      781 agaactcctg gctcaactaa cgatacgacc tgagcctatg aaagaagcag tgtctcaggg
      841 ttttctgctt gccacggagt tggcggacta tctggttaaa aaggggatgc ctttccgtga
      901 atcccaccac gtggtgggaa gtctggtacg ggagtgctta gcgaaaggga aggatttggg
      961 acaactcacc caacaggatt tactgaatgc ctcatcgtct tttgacgcca aggcatttca
     1021 agttttaact cccgaagctg cgattaacag taaaaacatt ataggtggta ccgctacagc
     1081 ccaagtcaca aaacagatca agggttggaa aaaatcatta caggccgaaa tgaaacattc
     1141 gatgaaaaga cagtgaacgg tatgaggcaa atcctaaaaa ttcctaccgg gtatttcctc
     1201 attttttgca tactggggct cctctcctcc tcaggttgcg gagcagtggg ccctcccatc
     1261 cccccagaaa aagttggcat tgaagccaag agactcaagc aacagcaaga tcaggccaaa
     1321 acagagggag cctttgacca ggatcccctc aatgtaccac tggaagaagc agtggaatta
     1381 cccaccgtgt atcctatcga tacccgataa tacacaggga tttgtccagg gagaacctca
     1441 cgaggtacaa tgaggccccc acagtctctc caactgtttg tggaggataa aagaatggat
     1501 acctctctca ctcagggtgc aattcgtccc aaacttctgt tgcttgatcc ttatccaaga
     1561 aataatccct atcgtatgac ggctagcgag cgccggtcga tctggtttcc gaagctcagc
     1621 ctaccagcga ttgccgccta tactccaaca acatgggatg tcgaaatcgt ggacgaggcc
     1681 gttagggata tcgattttga gactccctgt gatgtggttg gaatatccat tatgacgtgt
     1741 tatgccccga gagcctatga aatcgcggat gaatttcgtc gacgggggaa accggtcatt
     1801 ctcggaggag tgcaccccac ctattgccca gatgaagccc ttcgccattg cgatgccatt
     1861 gtctgcgggg aagccgaaga cctttggcct caagttatta ccgacatcga ggccgggtcc
     1921 ctgaaacgaa tctatcgcat ggaccaattt ccaaccttga gtcattacaa acccccgcgc
     1981 attgaattgc tcagcccgga ttcttacatg accaggcttt gcaccttcac gacgcgagga
     2041 tgtcattttg attgtgagtt ttgcagcgtt tcgccattta atggcaaaac cactcgacgc
     2101 cgacccgttc ccgaagtcat tgaagagctg aaacgagcca aagaatggct gcgctcggac
     2161 attattgaac gaatgacccg tgggtcactt ttccacgcct taacaacctc cctgaaaatt
     2221 tgggtaggcc tggaagaagg ttcgattgtt gcttttgttg atgacctgca taacagtcat
     2281 cgtgcctact gccgtgagtt atggcaggcc ctgaaatctc tcaatattaa atggggctgt
     2341 cagtccactc tctttcttgg ggatgatcct gaaatggtac gattggctgc cgatagcggt
     2401 tgcgtatcgg tttttgtcgg gatggaatcg ttgtcggatg attcgctcga tgaaaccaac
     2461 aaaggattca atcaggtgcg gaaatttgaa aaccaaatta aaatgtttca tgaccatggc
     2521 atcatggtga accccggtct cgtgtttgga tttgacaatg atgacgaatc ggtattcgag
     2581 actgccgtag aatttttgac acgaaacaag gtcgaattgg cctactttaa tgtgttgacc
     2641 ccccttccag ggacggcctt gttcgaacgc tttaagcggg agggcaggat cattgatcag
     2701 gattggtcga agtatgacgg gaagcatgtg gtatacacac ccaaacgcat gtcagccgag
     2761 caattacagg aaggattttt ctgggccaac caccagtttt attcctggcc ctgcatctgg
     2821 aaacgcatgg ggcataagag ccagcgactg attccccggc ttgaaatgaa ttgggaattt
     2881 cgaaaattga tataccgtac cacacccaaa ggcgctctat cccccctggc tacagtcttg
     2941 aaaaatctgc aaaccaagct cccgacatgt gaaacgcagc aactcgttcc taacgctctt
     3001 caccctcaac catctcctga ttccaaccac caggagctct gcttgaaaat gaaagctcgt
     3061 cgtcacgatg cctttgcggc cttgttcatt gatctggagg gaacattaga ccacctgaat
     3121 gccaaagaac ttttgaaaag gattacacaa gctggacgtg aggccaaaat ggatatcgtg
     3181 gtcaactttg agcacataaa acatgcgact ccgaaagcta tttacacatt acttgatggg
     3241 gaaattctcc gagccatcac cccccacaca aaattacggt atcgaaactt aaaagaagcc
     3301 tttcaatcca ttgtttctga aatcgctctt gaaaatttgg acctgtttga tgaggatatc
     3361 cagcatgcat gactttcact accaggggga cggattattc tgtgaggagg tccccattcg
     3421 ccaaattacc gaacaggtgg ggactccctg ctacatttat agccatcgga cattgattcg
     3481 acattttcat gcttttgatc aagccttcaa tgccattcca catatcgtgg cctttgccat
     3541 gaaagcgaat tcgaacctga ccgtcttgcg tctcctcgcc aaggaaggat gtggcgcaga
     3601 catcgtgtca ggtggagaac tttttcgcgc attaacagcc ggaatggcac ccaataaaat
     3661 cgtctttgcc ggggtcggca aatccaaaga agaaattcaa tatgcattgg aatcggatat
     3721 tttgatgttc aatgttgaat ctcccggtga acttcagcag atcaatgagg ttgctggctc
     3781 catgggcctt cgcgccaaag tggcgttacg catcaatccg gatattgatc cccaaaccca
     3841 tccgtacatt tctaccggat taaaaaaaag caaattcgga attggagcgg accgggcact
     3901 tgaagaattt gacgcagcgg ggaaccttcc tcacattgaa gtggtaggcg ttcattcaca
     3961 cattggttcc caattaaccc aggtcacccc atttgttgat gccttaaaaa aagctgtcgc
     4021 cttaattcaa acactgcaag ccaaaggtgt ccacattcag tatctcaaca taggcggagg
     4081 acttggtatt acttactcgg acgaaacgcc tccccatccc aaagagctgg ctgaagctat
     4141 ttccccgttg ctacaatccg tctcatgtca aattattatg gaaccgggcc ggtccattgt
     4201 agggaacgcg ggcatcttgg tgacgaaagt gctgtataac aaggcaagcg cggacaagca
     4261 tttcgtgatt gtcgatgccg caatgaatga tctgttacga ccaagcttat atgatgccca
     4321 ccacgatatt cagccggtct taaaaaaaga gtcatccgca gttaacactg ttgatgtggt
     4381 gggacccatt tgcgaatcag gagacttttt agcaaaagat cgaaaaatgc cccattctca
     4441 acctggagat ctcctggccg tcatgagtgc cggagcgtat ggatttacaa tggcttcaaa
     4501 ttataattcc cggccacgcg tccccgaggt gctcgtgaaa gggaaggaca tcacagtcat
     4561 tcgaaaacga gaaagctacg aggacctcat tcgaggcgaa accatttcag aagccttctc
     4621 gaattgattt ttcgatttaa agtcttgtgt tgattcgcca aggcgttcgc cgttttgtcg
     4681 ttacaaacac aaaaattccc cctacaccaa aagattgagg tcagaattat gttcagcggt
     4741 tcgttagtcg ctattgtcac cccattctca aagggaaagt ttgatgaaaa ggccatggcc
     4801 gatctcatcg aatcccaaat ttcttctggg acccatggcc ttgtaccttg tgggacgaca
     4861 ggcgaatcag ccaccttgac cccggaagag catgaacggg tcgtttcatt caccgtcgaa
     4921 gtggtgaata agcgggttcc tgtcattgcc ggaacaggct caaactctac cgatgaagcc
     4981 attactttta cgaaacatgc caaagccgtg ggtgccgatg gggcattgct catcacgccc
     5041 tattacaata agcccacgca ggaagggctt tatcgtcatt ttgccgccat cgccaaagcc
     5101 gtcgacttac cccagattct ttacaatatt ccagggcgga ccagcattaa catgttgccg
     5161 accacggtga gccgtctgtc ggaaatccag aatattattg gcatcaagga aggcagtgga
     5221 tctctccaac aggtatccga aattatacaa caatcccgac ctggcttcct tgttctctca
     5281 ggcgatgatc ccttgacact ccctatgatc gccctcggcg ggaaaggcgt gatcacggtc
     5341 accgcaaacg tcgccccgac caatatggca aacatggtga acgccgcact aaaaggtgac
     5401 tacgaaacag ctaggctcat tcactacaaa ctctctccct tatttggagc attatttttg
     5461 gagactaatc caattcccgt gaaagccgct ctggccatga tgggaaaaat gtcagaagaa
     5521 gttcgactgc cgcttacccc acttgctgcg gaatttcgac cagcactcca agaagccttg
     5581 caacaagccg gagtgctctg agaaaagaga acacatgatt cgaacaatca ttaccggcgc
     5641 ggccggtcga atgggcatgc gattagttgc gttaactcag gacacccctg ggctcaaact
     5701 ttccggtgca gtcgaagtaa aagggcaccc ctctattgga aaagacgctg gcgaagtcgc
     5761 ccaaatcggg caagtgaaca ttccgatcac cgacgatctc cacgtctgtc tctcccaggg
     5821 tgatgtggtg atcgacttca cggctccttc atcctgtctg gccaacatcc agcatgtcgt
     5881 caaatccacc aaggccatgg tcattggaac taccggattc actgatcaag aattggccca
     5941 acttaaaaca tttgctctga aaatcccctg tgtgttttcc ccaaacatga gtgtagggat
     6001 caatgtgctc ctcagcacca tcgggaaaat cgccagatct ctaggtgagc agtacaacat
     6061 cgaagtaatc gaagcccatc acaataagaa aaaggacgcc cccagcggaa ccgccctaaa
     6121 actggctgag gctctagcag aaggcatggc atgggattta caagaagtgg gcgtctatac
     6181 acgacacggt atgaccggcg aacggaaaac tcgtgaaatt ggcatgcaaa ccatccgcgc
     6241 aggagatatt gttggcgatc acaccatttt attgggggga ccaggagaac gcattgaaat
     6301 aacccaccgg gcccatactc gcgatacctt tgcccaaggc gcgcttcgcg cggcggaatg
     6361 ggtggctcaa aaaccacctg gcctctattc catggccgac gttttaggct tatcctaaaa
     6421 caacttcacg aatttatccg ccatatcggc atgaaagatg atggccaaca aaaaatttca
     6481 atcacaccca attaagctat acctcctcaa caacaattgc atgagaccta tcctctcaaa
     6541 tgtctccaag ccctactaaa cccggcctaa aagactccat tcatcctgtc gatacacaat
     6601 tctttcattg acaaagccaa agagcacgtg gtcttatcga aagagatt
//
LOCUS       DATIAL010000409         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1473230, whole genome shotgun sequence.
ACCESSION   DATIAL010000409 DATIAL010000000
VERSION     DATIAL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473230"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VLA60_12665"
     CDS             <1..1299
                     /locus_tag="VLA60_12665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF10260.1"
                     /translation="NNLVVTLFSLPVILVRLLTLRDQLRTGDLRVSDLVIMSSATGPE
                     DETPASETRHEAGQNFHKTLKHLDAIARLAKPFLSHFAQRLAMHGSAPDNFRTSSSFK
                     KPIQQLTKRIDALNLRPDVQETLIQGIHQIKEEILIHQQVLETSGRKQPGNASGKALA
                     RIREIEESLILMPHLEFLQSCEILEQTVAQVHQAKAKMVEANLRLVVSVAKHYTNRGL
                     HFLDLIQEGNIGLMRAVDKFDYTRGYKFSTYATWWIRQGITRSIAEKGNTIRRPVHIY
                     EITQKIKKTSQRLTQRLRRTPTLQELAENTGLPVAKVQGIMEGSYDPVSLDSPLDEQG
                     EGTLGDILEDHEAPSPLKISEEFSSKAAISRLLKALNPREERILRMRFGIGYDEESTL
                     EEIGQTLGVTRERIRQIETIALKKLREPVVRQQLESLLNN"
     gene            complement(1687..>2310)
                     /locus_tag="VLA60_12670"
     CDS             complement(1687..>2310)
                     /locus_tag="VLA60_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10261.1"
                     /translation="INFFKLTILSFIFTIGFLGGCSDSSTDKSHPESTSEHPGFMANI
                     DGAVHAEISGDGIVTYLPPKERDPVTGNRPGYYMLFNNLPTDAMEGRDLVIIFRIPDG
                     AQPGQYNLMTPDPLKVGQNFDVQVEAVEQGQSIPYQTNTEGTITLDNFTPDRISPEAS
                     RIKGTFQFVAENTVGEKVSVNGAFDFPSKQNVVSLNAGYSVKEELRI"
BASE COUNT          627 a          579 c          505 g          599 t
ORIGIN      
        1 aataacctgg tcgtgactct tttcagttta cccgtcatcc tagttcgtct ccttacgctt
       61 cgagatcaat tgcgcacagg agacctccgt gtcagtgatc tggtcatcat gagttccgca
      121 actggcccag aagacgaaac tcctgcatcc gaaacccgac acgaggcagg acaaaatttc
      181 cacaagactc tcaagcatct tgatgcgatc gcccggttag ccaaaccatt tttgtcccat
      241 tttgcgcaac gtctggccat gcatggttcc gcaccagata atttccgtac ttcttcctcc
      301 ttcaaaaaac ctattcaaca attaacaaaa agaatagatg ccttaaattt gcgccccgac
      361 gtacaggaaa ccttgattca gggaattcac cagattaaag aagaaattct cattcaccaa
      421 caggtcttgg aaaccagcgg ccgcaaacaa cccgggaatg cctccggaaa agctctggca
      481 cgtattcggg agatcgagga gtctcttatt ttgatgccgc acctggaatt tttgcaatcc
      541 tgtgaaatcc ttgaacagac cgtcgcccag gttcatcagg caaaagccaa aatggttgaa
      601 gccaatctgc gattagtcgt gagtgtggcc aagcactaca cgaaccgcgg gttacatttt
      661 ttggatttaa tccaagaggg caacattggg ttgatgagag cggtcgataa gtttgattat
      721 acgcgtggct ataagtttag cacctatgcc acatggtgga ttcgacaggg catcaccaga
      781 tccattgccg aaaaagggaa cactattcga aggcccgtac atatctatga gattacccaa
      841 aaaatcaaga agacctcaca gcgtcttact caacggttaa gacggacgcc gactcttcaa
      901 gaactggcgg aaaacaccgg gctaccggtt gcaaaagtgc aaggaatcat ggagggctcc
      961 tatgatcccg tttccctgga ctccccgttg gatgaacagg gtgagggcac attaggagat
     1021 attcttgagg atcacgaagc accctcaccg ttaaagattt cggaggaatt cagctcgaag
     1081 gcggccatct cccgcctcct taaagcattg aacccgcgtg aagaacgcat tcttcgcatg
     1141 cggtttggca tcgggtacga cgaagaatcc acattggaag aaatcggaca aaccttaggg
     1201 gtcactcgag aacggatccg gcagatcgaa accatcgcct taaaaaaatt gcgggaaccg
     1261 gttgttcggc aacaattgga gagtttacta aacaattgag cgtattgatt tcgtttcaca
     1321 accggatcaa ttccttttat ccggggtagg gaaagactta cgcctgtcac tcccataaag
     1381 agacgagaaa caaaatcccc cgcttgtcgt tcatgctttc ttggacaaca atcggaccgg
     1441 cctcattctc cttgagactc agcagacgtt agagcctctc aacatgatct ctcccaccag
     1501 gcgacatctc catgtctgtt ctgtccatag tggttggatg cttcgaatat ccttcgagcc
     1561 gcacttcatc acatgtattt aattttcttc tagaacgccc caccttccgc ggcatcgttt
     1621 tctgataacc ggaaaaacaa attgaaaaat ttgcaatgca tttctgggta atttcttatc
     1681 tttccttcag atccttaact cttccttaac cgaataccct gcattcaaag atacgacgtt
     1741 ctgtttcgag ggaaaatcga atgccccatt tactgaaacc ttttctccaa cagtgttctc
     1801 tgcaacaaat tgaaaggtgc ctttaatcct actggcttct ggagatatgc ggtcaggagt
     1861 gaaattatcc aaagtaattg ttccctccgt attcgtttgg tagggaatgg attgcccttg
     1921 ttctaccgct tctacctgta catcaaaatt ctgtcccacc ttgagaggat cgggtgtcat
     1981 caagttatac tggccaggct gggctccatc aggtattcga aatatgatga ccaaatctct
     2041 tccttccata gcatctgtgg gtagattgtt aaagagcata tagtaaccgg gacgatttcc
     2101 ggtcacggga tcccgttcct tcggtggtag gtaggtcaca attccatctc ccgatatttc
     2161 ggcatgaaca gccccgtcta tatttgccat gaatccggga tgttcactcg tggactcggg
     2221 atgactttta tcggtggatg aatcggaaca ccccccgagg aatcctatcg tgaaaatgaa
     2281 actaagtatg gtcagcttga aaaaatttat
//
LOCUS       DATIAL010000410         5455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_719674, whole genome shotgun sequence.
ACCESSION   DATIAL010000410 DATIAL010000000
VERSION     DATIAL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719674"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1020
                     /locus_tag="VLA60_12675"
     CDS             370..1020
                     /locus_tag="VLA60_12675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HSF10262.1"
                     /translation="MSQILQIVKHAQVSRSTFCPSKFRSTTLPNNIKYCAQCGSVIEE
                     KIPSGETLLRAVCPSCHTIHYENPKIVAGSIPEWEDKILLCRRAIEPRLGLWTFPAGF
                     MELGESTEEAAARETLEEANAEIVIHSLFGIFSLPHVSQVYVVYRAHLQNLNFKPGPE
                     SLDVQLVSLADIPWKDLAFPVIHESLTRYVKDIQTSTSQTHSSIVPIQKPPSFYSS"
     gene            complement(1268..1786)
                     /locus_tag="VLA60_12680"
     CDS             complement(1268..1786)
                     /locus_tag="VLA60_12680"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HSF10263.1"
                     /translation="MMKPPVPQKALKNSRISYWISPSPVGRLLLVGNPDGLQALQFQD
                     GAHPLDIQPTWTQSREPFRPVLEQLEEYFDGSRTRFQITLNLQGAPFQRHVWKALQRI
                     PYGRTVSYGEIAQQVGSPKASRAVGAANGQNPVSIIVPCHRVIGSNGKLVGYGGGLPI
                     KTALLALEQSKH"
     gene            1881..2348
                     /locus_tag="VLA60_12685"
     CDS             1881..2348
                     /locus_tag="VLA60_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HSF10264.1"
                     /translation="MSIQQHLHHWCQTRGLSLAELSQQTGVALTQLQDFQKGHWDPPL
                     STMALIAQSLNVPESWLHHDPRTLLRLWNDSDEDNPELPSTSSLDPLFQRIIQTSRDH
                     QDLFVLLTSLLHHGDPKLIRAAQVNLQSLFKQVRPTTVPWGSRPPGHFEPPND"
     gene            complement(2403..2807)
                     /locus_tag="VLA60_12690"
     CDS             complement(2403..2807)
                     /locus_tag="VLA60_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10265.1"
                     /translation="MNRILLIDDDIQVRESVSLSLETAGYTVMSAGSCQEGLQNHENF
                     QASLIIMDVLAMEEGEDEPTAPVSEQSSSIPMITLTGNIPASGGTMKGPHQIFQPICT
                     LQKPFTVDELLQTVQRALVGYVSSSPAPAPFS"
     gene            complement(3108..4439)
                     /locus_tag="VLA60_12695"
     CDS             complement(3108..4439)
                     /locus_tag="VLA60_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10266.1"
                     /translation="MTANTQALPASPSPFLTIFRMWWPLAASWMLMGLEFPLVTATVS
                     RLADPEVHLAAYGGVVFPLSLLIEAPVIMILVASTALCKDWLAYRLIRNFIVVLGSLL
                     TVVHVVVAFTPVYGMIVQDVLGVPDIIVEPARMGLQCMTPWTLSIAVRRFFQGVVIRA
                     GQTRLIGLGTLVRLFVSSAVLLCGLVLQSLPGIVVATVAMSSGAMVEAGFILFFVRPI
                     LTNLKMSPVHPSPLTLNQLRVFYWPLAFTPLISLGNLPIVSAAISRMPNAIESLAVWP
                     VLGGLSFVFRSGGLAMQEVVVALYDRPHFLVPLKKFVTLVSICTSGILFLIAATPLST
                     LWFEWGAALTPELTALASTALWLMVIFPALSPWESFFQGQFVHRGMTSVITQAVCLYL
                     FGSSIFLLAGVLYGQVTGLYVGLAAMLSGLGFQLFWLWRNSRRLDVHLHKF"
     gene            complement(4663..>5455)
                     /locus_tag="VLA60_12700"
     CDS             complement(4663..>5455)
                     /locus_tag="VLA60_12700"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HSF10267.1"
                     /translation="GKISAAELALQRFQFAARLMQAAGYAGWILLIDEAELIGRYSVN
                     QRAKSYAEVARLMNLDGGPTLPGLGAVLALTTDFREEVLEKKGDKVKLLEKLRAKNTE
                     ADRMLADKAEKGMGLLETQRIPLGQPPNDIIQESYQTIRSLHLKGHGWHMWENAANVP
                     AQIIPGTSMRKYVKSWVTHWDSLRFFPGEACEIEASTWKPSCVDQAPPQGANPEEDNQ
                     KKSHGGHFAVETDSTMAGKSEDQEFVAPGTPHTSGSFTNGPKTTF"
BASE COUNT         1544 a         1459 c         1243 g         1209 t
ORIGIN      
        1 tccattcaat cccaaaaccc cgatttttgc cccgtaaaaa aatgaaaggt aaatatcttt
       61 caagacctgt tttttcgggg gatgcatgcg gcccacaccc accaaggaaa aaataatttg
      121 tcgatcattc gtactcatgc cgttcgctcc aatcagttct cacgcgatcc aggatttgtc
      181 agggttgcaa gaagacacat gtagatgcca accttaccaa aggaatacga ggattcacaa
      241 tgcagactgg gaaaggtgac cctttccttc aaaatgtgac tgggatatgc tgcccttaaa
      301 ggaaagggtt tatcctaaaa cacgccattg aattacaggc aagacagagt atgataaaga
      361 aaagcccaca tgtcccagat ccttcagatc gtgaaacacg ctcaagtatc tcgctccaca
      421 ttttgtccat ctaaattcag atctactacc ttgcccaaca acatcaaata ctgtgctcaa
      481 tgtggaagcg taattgaaga aaaaattcct tcgggtgaaa ccctcctcag agccgtatgt
      541 ccatcctgcc ataccattca ctatgaaaac cccaaaatcg tggcgggcag cattcccgaa
      601 tgggaagaca aaattctctt atgccgacgg gccatcgagc ctcgtttagg gctctggacc
      661 ttcccggctg gatttatgga attaggcgag agcaccgaag aggcggcagc ccgggaaacc
      721 ctggaagaag cgaacgcgga aatcgtcatt catagtttat ttggaatatt tagccttcca
      781 catgtctctc aggtctatgt ggtctaccgg gcccatttac aaaatctgaa ctttaaaccc
      841 ggcccggaaa gcctggacgt ccaactggtt tccctagccg acatcccctg gaaggatctg
      901 gccttccctg tcatccacga atcgttaact cgatacgtca aggatatcca aacctccacc
      961 tctcaaaccc attcctccat cgtccccatt caaaagccgc cgtcttttta ttcatcctaa
     1021 ccgtcgactg acgaagaagt gctttagtca aaatgaatag tggaaaagga gagtggtggg
     1081 aatgatgttg ccctgcctga ctgccagaga agccgcactg attttccgtc ctgcccgctt
     1141 tcgcaccggc atttgcaata atcaattgga caacctagcg tgacaaaggg ttagcatgat
     1201 ggaaaatctc ctgttccagg cctgcgataa gttagggcag tgggaacggg cgaaaatgac
     1261 aggggggtta atgtttcgac tgttccaatg ctaatagggc agttttgatg ggtagtccgc
     1321 ctccatagcc aaccagtttt ccattagatc cgatcacccg atggcacggc acgataatag
     1381 aaacagggtt ttggccattg gcggctccca cggctcgtga ggccttcgga ctgccgacct
     1441 gttgagcaat ttccccatac gacaccgttc ggccataggg aatacgttga agagccttcc
     1501 agacatgacg ttgaaagggg gccccttgaa ggttcaatgt aatctgaaac cgtgtccggg
     1561 aaccgtcaaa gtattcctcc agttgctcaa gcaccgggcg aaacggctca cgcgactgtg
     1621 tccatgtcgg ctggatgtcg agagggtggg ctccatcttg aaactgcagg gcctgcaacc
     1681 catctggatt tcccaccaag agaaggcggc caaccgggct tggactaatc cagtaagaaa
     1741 tcctggaatt ctttaaggct ttttggggca ctggtggctt catcatcctg ctcctcacga
     1801 ttatcacagg tgaaaccgca tagggtcccg gacgaaattg aagatggaca ggctgaggaa
     1861 cattttccta tgatcgaacc atgtccattc agcaacattt gcatcattgg tgtcaaacca
     1921 gaggactctc tctagccgaa cttagtcaac aaacgggcgt ggccctgacg caattacagg
     1981 atttccaaaa gggccactgg gatcctccgt tgtccactat ggcgttaatt gcccaatcct
     2041 taaatgtgcc tgaatcctgg ctccatcatg atccccggac ccttctgcga ttatggaatg
     2101 actctgacga agataatcct gaacttccga gcacctcttc tctcgatccc ctatttcaac
     2161 gaattatcca aaccagccgt gatcaccagg acctcttcgt tttactcaca agcctgctgc
     2221 accacggtga ccccaagtta attcgcgcgg ctcaggttaa cctccaaagc cttttcaagc
     2281 aggtgcgccc caccactgtc ccctgggggt cgagacctcc aggacacttc gaacctccca
     2341 acgattaacc acccaaaccc tcttacccaa aggttgagtg gaaccaaaga aagaaatggc
     2401 tgttatgaaa atggggcagg agcgggagaa gaactaacgt aaccgaccaa agccctttgc
     2461 acggtttgga gaagctcatc aacagtaaag ggtttttgca gggtgcagat cggctggaat
     2521 atttggtgtg ggcccttcat cgttccacca gatgcaggaa tatttcccgt cagggtgatc
     2581 atcggaatac tcgaagattg ctcagaaaca ggtgcagtgg gttcgtcttc tccctcttcc
     2641 atcgccaaca catccataat aatcaaggac gcttgaaaat tttcatgatt ttggaggcct
     2701 tcctggcagg agcctgcgga catgaccgta tacccggccg tttccaaaga aagagagacg
     2761 gattccctca cctgaatatc atcatcaatc agcaaaattc gattcataag ttaaccaaaa
     2821 ggtattcaca aataaatatt ctcgttacca tttcaaaaaa ggcaattttc ttcatcacat
     2881 aaacgagcaa tttatgttcc ttcccgactg caattgcctt gaagtaattt tcactttaaa
     2941 aaacaatgac ttataccatc atttcagaat tggtttgtga gccacatgaa ggaaaaaaca
     3001 tgcaatatgc agggattatc attcactgtt ttcccaaaaa tcccctaatg aacctcaaga
     3061 ccaccggact ggtcctgaaa ccaccaaagc aacccaccaa aaaataccta aaatttgtgt
     3121 aaatggacat caagcctgcg actgttcctc catagccaga atagttgaaa tcctaacccg
     3181 gaaagcatcg cggccaagcc cacatataat ccggtcactt gcccataaag aactccggcc
     3241 agcaaaaaga tggaacttcc aaacagatac aaacacacgg cttgagtaat aacagaagtc
     3301 atcccacgat gaacaaactg gccttggaaa aaactttccc atggactcaa agcgggaaaa
     3361 atcaccatga gccagagcgc tgtggaggcc aaggcggtca gctcaggagt cagagccgct
     3421 ccccattcaa accagagagt ggagagagga gtagcagcaa tcaaaaaaag gattccgctc
     3481 gtacagatgg aaaccagcgt cacaaatttc ttcaaaggga cgagaaaatg cggtcggtca
     3541 tacagggcga caaccacctc ctgcatcgcc aatcctccac tccgaaacac aaacgagagc
     3601 ccacccaaca ccggccagac agccaaggac tctatagcgt ttggcatccg actgatggcc
     3661 gcactgacaa tcggtaaatt cccaagggaa atgagtggag taaatgcgag tggccaataa
     3721 aacacgcgta attgattcag tgtgagaggc gatggatgca caggactcat ttttagattg
     3781 gtgaggatcg gacgcacgaa aaacaaaata aagccagcct cgaccattgc cccagaagac
     3841 atcgcgaccg tggccacgac aatccccggc aaactctgca gaaccaatcc gcaaagtaag
     3901 accgcactgc ttacgaataa acggaccaag gttcccagcc caatcagacg cgtctgccct
     3961 gcccgaatca ccactccctg aaaaaacctt ctcaccgcaa tcgagagcgt ccatggcgtc
     4021 atgcattgaa gacccattct ggccggttcc acaataatat ctggaacacc taacacgtct
     4081 tgtacgatca ttccgtacac cggcgtaaag gccacgacga catgaacgac ggttagaaga
     4141 cttcccaaga ccacaatgaa atttcgaata agccggtagg ccaaccaatc cttacataag
     4201 gcggtagacg caaccagaat cataatgacg ggtgcttcga tcaacaatga gagaggaaaa
     4261 accactccac cataggcggc taaatggacc tcaggatcgg ccaagcggga aaccgtggct
     4321 gtgaccagtg gaaactccaa acccatgagc atccaactcg cggccagggg ccaccacatg
     4381 cggaaaatcg tgaggaacgg agaaggagag gcaggtaggg cctgcgtgtt agcagtcacg
     4441 aaacgacaag cggatcacag ggaacccata tgtacactgt ccaaaatgag caggaagcct
     4501 gagaggaggc aaaaggaaaa caggttcatt ggcaaatgga tacggctacc tattcgggga
     4561 aagtaaagta aggcgtcatt cacgaaaggg aaccgccctt tcgtgaatga cgcctacctt
     4621 atcatgaacg cgaggtgttc cgccaaaagg tcggaacctg agttagaagg tagttttagg
     4681 gccattcgtg aaggagccag aggtgtgtgg agtcccgggg gcaacaaatt cctggtcttc
     4741 agattttccc gccatggtcg aatcggtttc caccgcaaaa tgccccccat ggcttttctt
     4801 ctggttatcc tcctccgggt tggctccctg ggggggggcc tgatcgacac aagacggctt
     4861 ccatgtcgaa gcttctattt cacacgcttc tccaggaaag aaacgaagtg aatcccaatg
     4921 tgtcacccaa ctcttcacat atttccgcat actggttccc gggataatct gggccggaac
     4981 atttgccgca ttttcccaca tatgccaccc atgccctttc agatgaagcg atcgaatcgt
     5041 ctgataggat tcttgaataa tatcgttcgg tggctgtcca aggggaatac gttgggtctc
     5101 caataagccc atgcctttct ccgctttgtc ggctagcatc cgatcggctt ccgtattctt
     5161 ggctcgaagt ttctccaaaa gtttgacctt gtcacctttt ttctctaaga cctcttcccg
     5221 aaaatcagtg gttaatgcca gcacagcacc caatccgggg agagtaggac cgccatctaa
     5281 attcatgagg cgtgccactt cggcatagga tttcgcccgc tggttcaccg aataccggcc
     5341 aatcagttcg gcttcatcaa tcagcagaat ccacccggca tatcccgcag cttgcatcag
     5401 acgagcagca aactgaaagc gctggagcgc caattctgca gccgatattt ttcca
//
LOCUS       DATIAL010000411         3856 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_612976, whole genome shotgun sequence.
ACCESSION   DATIAL010000411 DATIAL010000000
VERSION     DATIAL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612976"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VLA60_12705"
     CDS             <1..349
                     /locus_tag="VLA60_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10268.1"
                     /translation="KGKVPAGTTLPIVDGVTSTFMTQHQAEGHRRAKRGIHIAALLSQ
                     QFSGRPRIDVSQPHTTIVDLPNVRFQFGREVEDQWQRFLVLYPTVKDKIEGRSQEVDL
                     RFAQKVIFRKRTL"
     gene            353..1606
                     /gene="ftsA"
                     /locus_tag="VLA60_12710"
     CDS             353..1606
                     /gene="ftsA"
                     /locus_tag="VLA60_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HSF10269.1"
                     /translation="MTKKEHIVVGLDIGTTKICAVVGEIQDDRSIHVIGVGSHRSNGL
                     RKGMVVNMDSTVESIKRAVEEAELMSAVQINSVYTGIAGSHIGSESAQGVVALKKREV
                     TKADVRRAVDTARACAVPAPDRQILHVLPREFIVDDQEGIRDPLGIAGSRLEVDVHIV
                     TGAVTSAQNLIRCVSRAGLDVVDIVLQPLASSAAVLTHEEKDLGVVMVDIGGGTTDIA
                     IFVEGCIRHSAVLPIGGSHLTGDLAMGLKTAPEEAEKIKLKYGVASSLFVNEDEGIEV
                     PSVGGRPPRVMPRALVAEILSPRVEEMFELVLREIRRAGYDGMLVAGGVLTGGTSLLP
                     GMAEVAEHVLNLSVRLGRPIGVEGLREIVSNPSYSTAVGLIVHSVSHENEFELVGPGA
                     GKGKKSGRWAGGLVGKMKGWIMNFF"
     gene            1719..2915
                     /gene="ftsZ"
                     /locus_tag="VLA60_12715"
     CDS             1719..2915
                     /gene="ftsZ"
                     /locus_tag="VLA60_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HSF10270.1"
                     /translation="MFSLSDEEQSVINIKVVGVGGAGCNAVNTMIEAGLSRVEFVVAN
                     TDLQALGRSLASYKIQLGPERTRGLGAGAKPEVGKESAMESEHQIREALEGADMVFVT
                     AGMGGGTGTGGAPVAAKIAKELGILTVGVVTKPFQYEGNRRTSFAEEGIRELKKYVDS
                     LLIIPNQKLLGMVDKSTPLVEAFKIADDVLRQAIQGISDVITTPGLVNVDFADVRTVM
                     GYSGRAVMGMGTGRGPTRASDAARLAIASPLLEDGSVEGARGLLLNITGGPNLTLHEV
                     NEASNIAREAADPQANIIVGQVINPELGDELTVTVIATGFEQAESVMRFSNAHQPTLV
                     VEPVKKPALVAVPAENGASNGSEDLDRPTYLRRQATARPVPEKASLLVDDDWDVPTFL
                     RRKAEN"
     gene            2920..3675
                     /gene="pgeF"
                     /locus_tag="VLA60_12720"
     CDS             2920..3675
                     /gene="pgeF"
                     /locus_tag="VLA60_12720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HSF10271.1"
                     /translation="MTMEKGPFPNGSGVSDDGLTSDMIPVRHFFGTRSTPVGLATHIQ
                     LGHIASGPREFPAVVALKQIHSTRVVVIHTHRGLGTLEQTEGDALVTNQPETLVVVRT
                     ADCVPVLLVEKARGVVGAIHAGWRGAVGGIVAETIRSCVNEFGAKPEHMHLAIGPSIG
                     PCCYEVDEQVINPLRSRYPAWPGVLQETHEGKGVLDLKQLIYHQIRAGGVPDSQIGRV
                     DHCTHCRDDLFYSYRREGQVNGTMYSGIILPAA"
BASE COUNT          889 a          891 c         1244 g          832 t
ORIGIN      
        1 gaaagggaag gtgccggcag ggacgacatt gcccattgtt gatggggtga cctccacatt
       61 tatgacacag catcaagccg aaggccatcg acgcgccaag cggggtattc acattgctgc
      121 gttgctgtcc cagcagtttt cgggcagacc acggattgat gtgtcgcaac cccatacaac
      181 gatcgtggat ctacccaatg tccggtttca attcgggcgt gaggtggagg accagtggca
      241 acgatttttg gtgttgtatc ccaccgtgaa ggataaaatt gaaggtcggt cgcaagaagt
      301 ggatttgcga tttgctcaaa aagtcatttt tcgcaagagg acactatagg acatgaccaa
      361 aaaagaacac attgtcgtgg gactggacat cgggacaaca aaaatttgtg cggtggtggg
      421 ggaaattcag gatgatcgct ccattcatgt gattggagtg ggctcacatc gttcgaacgg
      481 gttgagaaaa ggcatggtcg tcaacatgga cagtaccgtg gagtccatca agcgggccgt
      541 ggaggaagca gaattgatgt cggcggtcca aatcaattcg gtgtatacgg ggattgcggg
      601 aagccatatc gggagtgaga gtgcgcaagg cgtggtggcc ttaaaaaaac gggaagtgac
      661 caaggcggat gtccgtcggg ccgtcgatac cgcacgagcc tgcgccgtgc cggctccaga
      721 ccgccaaatt cttcatgtgc ttcctcggga atttattgtg gatgatcagg aagggattcg
      781 cgatccatta ggcattgcgg gatcacggtt ggaagtcgac gtgcatattg tgaccggagc
      841 ggtcacctcg gcacaaaacc tgatccggtg tgtcagccgg gcgggattgg atgtcgtcga
      901 tattgtgttg cagccgttag cctccagcgc ggccgtgctg acccatgagg aaaaggacct
      961 gggggtcgtg atggtggata tcggaggagg caccaccgat atcgcgattt ttgtcgaggg
     1021 atgtatccgg cactcggcgg ttcttcccat cggggggagc catctcaccg gggatctggc
     1081 aatgggattg aaaaccgcgc cggaggaggc ggaaaaaatt aaattgaaat atggggtggc
     1141 cagtagtctc tttgtgaatg aggacgaagg cattgaagtg ccgagtgtcg gaggacggcc
     1201 gccccgggtt atgcctcgag cgttggtcgc ggagattctt tctccacggg tggaagaaat
     1261 gttcgagctc gtgcttcgtg aaatccgtcg tgccggatat gacggcatgc tggtggctgg
     1321 aggcgtgttg acgggcggaa cctccttact cccgggcatg gccgaagtgg cggaacacgt
     1381 gctgaatctg tcggtgcggt tagggaggcc aatcggagtg gaaggtttac gggaaatagt
     1441 cagtaaccca agttactcga cggctgtcgg gcttatcgtc cattcggtga gtcacgaaaa
     1501 tgagtttgaa ctagtgggcc ctggagccgg caaaggcaaa aaatccgggc ggtgggccgg
     1561 tggtctggtc gggaaaatga agggatggat catgaacttt ttttaaatta tgacaagaaa
     1621 ggaggatcat ggatccgtgc ctacttcgcg agccaaggtg agtcatcatt caaatgtccc
     1681 cacgggctac caggggagtg gtagaaagga ggagtcgtat gttttcactg tctgatgagg
     1741 agcaatcggt tattaacatc aaagttgtcg gtgtcggagg tgccgggtgc aacgcggtaa
     1801 atacgatgat tgaagccggc ttaagccggg tagaatttgt cgtggccaat accgatttgc
     1861 aggccctggg ccgctcgctt gcgtcgtaca aaatccaatt gggtccggag cggacgcggg
     1921 gcctgggggc aggagccaag ccggaggtgg gaaaagaatc ggcaatggaa agtgaacacc
     1981 aaatccgtga ggccctggaa ggggcggata tggtctttgt cacagcaggc atgggagggg
     2041 gaacgggcac gggcggtgct cccgttgccg caaaaattgc caaagagttg ggcatcctca
     2101 cggttggagt agtgaccaaa cccttccaat atgaaggaaa ccggcggacc agctttgccg
     2161 aagaagggat ccgtgaatta aaaaaatacg tggattccct cttgatcatc cccaaccaga
     2221 aactcctggg aatggtcgat aaaagtacgc cgcttgttga ggcgtttaag attgcggatg
     2281 atgtgttgcg gcaggccatt caaggtattt ctgatgttat taccactccg ggtttggtca
     2341 atgtggattt cgccgatgtg cgaaccgtca tgggctattc cgggcgtgcg gtgatgggga
     2401 tgggaacggg ccggggtccg acgcgagcca gcgatgcggc tagactagcc attgccagcc
     2461 ccttgctgga agatggaagc gtggaagggg caagagggtt gttgttgaat attaccgggg
     2521 ggcccaatct cacgttgcat gaagtaaacg aagcgtccaa tattgcccgg gaagcggcag
     2581 atcctcaagc caatatcatc gtaggtcagg tcattaatcc cgagctaggg gatgaattga
     2641 ccgtgacggt cattgccaca ggattcgaac aggccgagtc ggtcatgcgg ttttccaatg
     2701 cgcatcaacc gactcttgtt gtggagcctg ttaaaaaacc cgcattggtg gctgtgcctg
     2761 cggaaaatgg ggcgagcaac ggttctgagg atctcgaccg gccaacctat ctgcgacgac
     2821 aggcgactgc caggccggtt cctgaaaagg ccagcctgct ggtggatgat gattgggatg
     2881 tgccgacttt tttacggagg aaagcggaga actaatccca tgaccatgga gaaaggtccc
     2941 ttcccaaatg ggtccggagt gtctgatgac ggattgacga gtgatatgat ccccgtgcgg
     3001 catttcttcg ggacccggag tacgccggta gggttagcca cccatataca actcgggcac
     3061 attgcgagtg ggccgcggga gtttcctgct gtcgtcgcgc tcaagcaaat ccatagtacc
     3121 cgggtggtcg tcatccacac ccatagagga ctagggacat tagagcagac cgagggtgac
     3181 gcgttggtca ccaatcaacc tgagaccctg gtggttgtgc gcacggcgga ttgtgtcccg
     3241 gtgctgctgg tcgagaaggc caggggagtg gttggtgcca ttcatgccgg atggcgcggc
     3301 gccgtaggcg gcatcgtggc agaaaccatt cgttcttgcg tgaacgaatt cggggcaaag
     3361 ccggagcaca tgcatctggc gatcggcccc tctatcggac cgtgttgtta cgaagtcgat
     3421 gaacaggtca tcaacccgtt gcgaagccgg tatccggcat ggcccggggt gcttcaagaa
     3481 acccacgaag gcaaaggggt tttagacctc aagcaactga tctaccacca gatccgcgcc
     3541 ggtggcgttc ctgactctca gattggacgg gtggaccatt gcacccattg tcgggacgat
     3601 ctcttttatt cctacaggcg ggaggggcag gtgaatggga ccatgtatag tggaattatc
     3661 ctgcctgctg cctaaggatt gatctcgctt gccgggagtg gcaaggtttt atgcgatgta
     3721 gggacgcatc gctgacccat cctaagcgat tcgccgcttt tacgcgcgga gcgtacgctt
     3781 agtacgtgag cacggaaaaa tggcgagaac gccgctggcg gcttttttca acagacccat
     3841 ctagggactc acagga
//
LOCUS       DATIAL010000412         2609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_720024, whole genome shotgun sequence.
ACCESSION   DATIAL010000412 DATIAL010000000
VERSION     DATIAL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720024"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(433..2037)
                     /locus_tag="VLA60_12725"
     CDS             complement(433..2037)
                     /locus_tag="VLA60_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HSF10272.1"
                     /translation="MSNQLMSKHVIPYVPGLTKTIISLILVGTVCLSFPSQSLAYKIE
                     IGVSGPIGGDSVTEENGRTEIQLDTGPVIGPDGEAGSAYATANLATGTLALKVTSTTP
                     VQSSQEGMGLASIQINDRLRFTLDPGVAIANIRFSMRVDGFPSCRDLSSNCFTNAIIR
                     LGEMSDQALGLPLPEPKELEVRVTVVDGQELPIEAFLSARVEMNNLIDVSDPSATIRL
                     ILPKGVSFTSESGVFLGLQPPFEEPPPRAPVSSDLNGDGKADIVWRNVRTGEVAVWLM
                     NGGLIGSSGFLGGVSSDWQIVGVGDVNNDGMADIVWRNTLSGAVAVWFLNGLTITSAS
                     FPGSASLDWVIKGVGDMDGNGSADLIWRNMQNGAVAVWLMDGPSISRFRSLPGVPLAW
                     QIVGIGDVNADGLADIIWRHNGGVSAVWVMDESTITSVGFLGGTTPEWEKAGFGDLNG
                     DGTTDMVWRHTGTGKVGIWLMNGGAIEVSGFLPGMLPGWRIAQVGDADGDGKADVIWH
                     NRSTGQVAVWLMDGLTLSSTEFPGST"
BASE COUNT          710 a          740 c          576 g          583 t
ORIGIN      
        1 ttggggaacg gaatattggg ttgaatggct gcctcttcaa tgtcggcata tccttttttt
       61 ccccgtgaga tattgtcgct caagaatccg caattcttgg gtactcgcct gctcgtgccc
      121 acactctgaa aaaaatcggt ctccaccttt cccgtggttg tcgcctctag agccatccag
      181 aaaatttttg aacacaccaa atggagggca aaccggggga aacgggaatc aacttgtaag
      241 aggggaaaac gctactataa tgggtctctc tccctaacga ggatttgaat aaccacggaa
      301 ttcagagcag gcttcaagga atcggatttg cccattttca atgggggaat tgagaattag
      361 gacaaagaag tgatcgttaa tagaggagct taccgaacga aagctctgta gggatctcct
      421 gtgacagtat acctacgtgc tgccgggaaa ttctgtcgag gacaaggtca ggccgtccat
      481 gagccagacc gccacctgtc ctgtactgcg attatgccag atcacatcgg ccttcccatc
      541 tccatctgcg tcaccaacct gggcaatacg ccaccccgga agcatacccg gcaaaaagcc
      601 ggatacctca atcgcccctc cgttcatcaa ccagatcccc actttcccgg taccggtatg
      661 acgccagacc atatcagtcg tcccatcgcc gttcaggtca ccaaaacccg ctttctccca
      721 ttctggtgtg gtccctccga ggaatcccac cgaggtgatc gtcgattcat ccatcaccca
      781 cacggcagat acccctccgt tatggcgcca aatgatgtcc gctagaccat ccgcattcac
      841 atcacctatc ccaacgatct gccacgctaa gggcacaccg gggagtgaac ggaatctgct
      901 aattgagggc ccatccatga gccagaccgc aacagctccg ttttgcatat tgcgccagat
      961 gagatcggca cttccattgc cgtccatatc gcccactccc ttaatcaccc aatccaagga
     1021 agcgctcccg gggaaactgg ccgacgtgat cgtcaacccg ttcaaaaacc atacagccac
     1081 cgcaccacta agagtgttgc gccagacaat atcagccatt ccgtcattat tcacatcacc
     1141 cactccaacg atctgccaat cagatgagac cccacccagg aaaccagacg aaccaatcaa
     1201 gcctccattc attaaccaca ccgccacctc accggttctc acattgcgcc agacaatatc
     1261 cgccttgcca tctccattga gatcggaact gacaggggca cgagggggcg gttcctcaaa
     1321 aggaggctga aggccgagga acaccccaga ttccgaggta aagcttaccc cctttggcag
     1381 aattaatcgg atagtcgcac ttggatcgct gacatcaatg aggttattca tctcgaccct
     1441 ggccgataaa aacgcctcaa tgggtagctc ctgcccatca acaactgtga ccctgacctc
     1501 caactccttt ggctccggta aaggaagtcc aagagcctgg tcactcatct ctcccagacg
     1561 aatgatcgca ttggtaaagc aattggaaga caaatcccga caagagggaa acccatcaac
     1621 cctcatagaa aatctgatgt tggcaatggc cacacccgga tccagcgtaa acctcaaccg
     1681 atcattgatt tgaatggaag ccaatcccat gccctcttgc gaactttgca cgggagtcgt
     1741 cgaggtcacc ttaagcgcga gggttccagt ggctaaattg gctgtggcat aggcagagcc
     1801 ggcttcaccg tctggtccga ttaccggacc cgtgtccaat tggatttcag tacgtccatt
     1861 ttcctcggta actgaatccc ccccaatcgg ccctgaaaca ccaatctcga ttttatacgc
     1921 gaggctctgt gacgggaagg acagacacac cgttcccacc agaatgaggg atatgatggt
     1981 cttggtcaga ccgggtacgt agggtattac gtgcttcgac attaattgat ttgacataaa
     2041 agattacatg aacaaggttg actaacctta tcggatagac aattccattc ttaaaaatca
     2101 ggcaccccat tccagatctt ccctccctcg ccatttcttt attttcttac cagactcctc
     2161 aaaaacgttg gaaattctct ctcgcggaga acacaatgga gtcattcctc ccttatattt
     2221 caaaggccat tttcctatgc ggtcatcaat ttcaaaaatc accaatagga gtcaatctta
     2281 tctctagtct cagcctttct attaagactc aaccggaaat tagggagatt ccggatcgaa
     2341 agcagaaggg gcccgaatag ctgttcattt cttcaatccg aaaaaaaggc atcaggtttg
     2401 gatgaagggg gtaggagtca tcctacgcgg aaaagttgga tcacgaagcg gggcgcataa
     2461 accagacgaa cacgttttta taatttagca ggagagaaca agccattctc gtagaagagg
     2521 aaatccaaca cagcaatggg aacaaggggt ttgtcccccc ccccaaaaaa tcggagagat
     2581 gcgttgatat gccacagagg atcgatgaa
//
LOCUS       DATIAL010000413         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_75343, whole genome shotgun sequence.
ACCESSION   DATIAL010000413 DATIAL010000000
VERSION     DATIAL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75343"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /gene="murC"
                     /locus_tag="VLA60_12730"
     CDS             <1..975
                     /gene="murC"
                     /locus_tag="VLA60_12730"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HSF10273.1"
                     /translation="FVIGGKVNAMGTHARLGRSDLLIAEADESDGSFLRLSPSIVVVT
                     NIDREHLDHYGSMEGLQEAFLEFINKIPFYGVAIVCADDPWIRKLLPRVVKRYHTYGM
                     SDFSGVLTSDLFATDIETKAMGVEFRAHYRDQKLGPFRIRIPGVHNVANALAAIGVAL
                     ELDVPVDLIRAGLAAFAGVERRFQIRGEKNGIVVVDDYGHHPTEIRATLAAARAAWQR
                     PLIVVFQPHRFSRTRDLVDEFAKAFEQADRVYVMDIYPAGEAPIPGITGQMMADTIRA
                     SGHPSVQWLNRDSGLITKLREGLREGDVLLTLGAGDVWKVGTELLESL"
     gene            1014..1934
                     /gene="murB"
                     /locus_tag="VLA60_12735"
     CDS             1014..1934
                     /gene="murB"
                     /locus_tag="VLA60_12735"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HSF10274.1"
                     /translation="MKTGRPTLTAKRLQRAVQHVQGEVHWNESLAPLLSLQVGGPADV
                     LIFPLDVEDVIRVIVGARTEGIPFVVLGGTNVVVRDKGIRGIVMQLKHLTGIHEEADH
                     VVYAQAGVRLPRLMQFAVGHHLSGMEWAAGIPGTVGGGVVMNAGTRLGEMQEVLHAVD
                     LVNLRGHRVRIEASNMSFTYRKATLPKGVVVGAWVQLALSDKAQVEAKTKTYLQYRRE
                     TQPLTLPNAGSVFKNPPGDSAGRLVEAAGLKGVRIGDAEVSTKHANFIVNRGAATADQ
                     VLALIRKVRQTVARKFGVRLQLEWKIIGES"
     gene            2012..2980
                     /locus_tag="VLA60_12740"
     CDS             2012..2980
                     /locus_tag="VLA60_12740"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HSF10275.1"
                     /translation="MMTQKSLRIGVLMGGSSAEREISLKTGRSICDALKRRGYTVIPI
                     EVDATLPHQIRAKKISVAFLALHGPGGEDGTVQGMLEVMKMPYTGSGVSASAVCMNKG
                     LTRVVLQAAKVPVPPGMTVSGKMIPVRPPPSLGLPVVVKPCAEGSTFGVSIVRKPSQW
                     KEALQEAYRYGEQAVVEKYIPGREVAVGVFGNEVLPGVEIIVPGGFYDFTAKYGKAAT
                     RYVCPASLSSKLETALREYSLRAYQALGCRGAARVDFRIHTNGRPYILELNTIPGMTE
                     RSLLPMAAAQIGWDYDDLVERILREALPKRMVPSSGKIRSTRSSAS"
     gene            2977..>3316
                     /locus_tag="VLA60_12745"
     CDS             2977..>3316
                     /locus_tag="VLA60_12745"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HSF10276.1"
                     /translation="MISGESLNTKRPRKNRPLKKTGGPGKAKSSSTALRLRRVVIGGA
                     LACLIVVVVVGGRQVMRWADEWTEIQQITVVGLDRVTRDEILTKLDLAPQTSLFSVDS
                     ELLATRLESHP"
BASE COUNT          708 a          753 c         1085 g          770 t
ORIGIN      
        1 ttcgtcattg ggggcaaggt gaatgccatg gggacccatg cgcgtttggg ccggagtgat
       61 ctgctgattg cggaagccga cgagagtgac gggtcgtttt tgcggttgtc accctccatc
      121 gtggtcgtga cgaatatcga ccgtgagcac ctcgatcatt acggaagcat ggaaggtctt
      181 caagaggcgt ttttagaatt tatcaataag attccattct acggcgtggc gattgtgtgt
      241 gccgatgatc cctggattcg caagctcctg cctcgggtgg tgaagcgata tcacacgtat
      301 ggcatgagtg atttttcggg tgtgctgacc tctgatttgt ttgcgacgga cattgaaacc
      361 aaagcgatgg gtgtggaatt tcgggcacat taccgggatc aaaagcttgg gcccttccgc
      421 attcgtatcc cgggtgtgca taacgtggcc aatgcgctag cggccatcgg ggtggctttg
      481 gaattggatg tgcccgtgga tttgatccgg gcagggttgg cggcttttgc cggagtcgag
      541 cggcggttcc agattcgtgg agagaaaaac gggattgtgg tggtggacga ctatgggcac
      601 catcctaccg agattcgtgc caccttggcg gctgcgcgag ctgcgtggca gcgcccgttg
      661 atcgtggtgt tccagccgca tcggttttca agaaccaggg acctggtcga cgagtttgcc
      721 aaggcgttcg aacaggcgga tcgcgtgtat gtcatggata tttatcccgc cggtgaggcg
      781 ccgattcccg ggatcacggg acaaatgatg gccgacacca ttcgggcgtc tggtcatccc
      841 tctgtccaat ggctcaatcg tgattccggt ttgatcacca aattgcgtga ggggcttcgt
      901 gagggcgatg tcctgttgac gctgggtgcg ggcgatgtct ggaaagtcgg aactgaattg
      961 ttggaatcgt tgtaaccgaa tgtggtgagt catggaagtc gaggattgta caggtgaaga
     1021 ccggccggcc gacactgaca gcgaaacggc ttcaacgagc cgtgcagcat gtgcaaggag
     1081 aagtccactg gaacgaatcc ctggcgcctc ttctgtcgtt gcaggtgggc ggccctgcgg
     1141 atgtcctgat ctttcccctg gatgtggaag acgtcattcg cgtgatcgta ggggctcgaa
     1201 cagaagggat tccctttgtg gtgttaggcg ggaccaatgt ggtcgttcgc gataaaggga
     1261 ttcgaggcat tgtcatgcaa ttgaaacact taaccgggat acatgaggaa gccgatcacg
     1321 tggtatatgc ccaggcgggg gtgcggttgc ccaggttgat gcaatttgcg gtggggcatc
     1381 atctgtccgg aatggagtgg gcggcgggga ttccgggaac cgtcggtgga ggggtggtga
     1441 tgaatgccgg aacgcggttg ggggaaatgc aggaggtctt gcatgccgtc gatttggtga
     1501 atctgcgtgg acatcgcgta cgaatcgagg cttccaacat gtcatttacc tatcggaagg
     1561 cgacgctccc caaaggcgtc gtcgttggcg cgtgggtcca gttggctctc tcggataaag
     1621 cgcaggtgga ggcgaaaacc aaaacctatc tgcagtaccg gcgggagacc caaccgttaa
     1681 cccttccgaa tgccgggtcc gtgtttaaaa acccgccggg agattctgcc gggcggttag
     1741 ttgaagccgc aggattaaag ggtgtgcgga ttggtgatgc tgaagtatcg accaaacatg
     1801 ccaattttat tgtgaataga ggagcggcca ccgccgacca ggtactggca ttgattcgaa
     1861 aagtccggca aaccgtggcg agaaagtttg gggtccgtct tcagcttgaa tggaaaatta
     1921 ttggagaatc gtagagatgc cggggcttgt tggtggtgac gaagagatgc agtcagagcg
     1981 aaaggcttat cacaaggtga agacacagag aatgatgaca cagaaatctt tgcgaatcgg
     2041 tgtgctgatg gggggatctt cagctgagag agagatttcg ctcaagacgg ggcgttcaat
     2101 ctgcgatgcg ttgaaacgac gtggatatac ggtgatcccc attgaagtcg atgccacatt
     2161 gccgcatcag atccgggcaa agaaaatttc ggtggccttt ctcgcgttgc atggaccggg
     2221 aggggaagat gggacggttc aagggatgtt ggaagtcatg aagatgccgt acaccggatc
     2281 gggcgtgagt gcgagtgccg tctgcatgaa caagggattg acgcgcgtgg tccttcaggc
     2341 tgccaaggta cccgttccgc cgggcatgac cgtatccggg aaaatgatcc cggttcgtcc
     2401 acctcccagc ttaggcttgc ctgtggtggt gaagccttgt gcggaggggt ccacgtttgg
     2461 tgtttcgatc gttcgcaagc catctcaatg gaaggaggcc ttgcaagaag cctatcgata
     2521 tggggagcag gcggtggtgg aaaaatatat tcccggccgg gaagtggctg tgggcgtttt
     2581 tggcaacgag gtcttacccg gcgtagaaat catcgttccc gggggctttt acgattttac
     2641 ggccaaatat ggaaaggcgg caactcgtta tgtgtgtccg gcttcgcttt cttcaaaact
     2701 ggaaaccgct ctccgtgagt acagccttcg tgcctatcag gcgttagggt gtcgcggtgc
     2761 cgcccgagtc gattttcgca ttcataccaa cggacgtccg tatattctcg aattgaacac
     2821 tattcccggc atgactgaac ggagcttgct tcccatggca gcggcacaga tcgggtggga
     2881 ctatgatgat ctggttgaac ggatattgcg ggaggctcta ccgaaaagga tggtaccgtc
     2941 ttccgggaaa ataaggagca ccaggagttc tgcgtcatga tatcgggaga atcccttaac
     3001 acgaaacggc ctcgaaaaaa ccggcccctt aagaaaaccg gaggacctgg gaaagcgaag
     3061 tcgtcatcga cagccttgcg gctccggcgt gtcgtgattg gaggtgcctt ggcctgtctt
     3121 atcgtggtgg ttgtggtggg aggacgtcag gtgatgcggt gggcggatga gtggacggaa
     3181 atccaacaga tcacggttgt gggtttagat cgagtcacgc gcgatgaaat tctgacgaag
     3241 ctggacttgg cgccacagac cagtttgttc tctgtggatt cagagctgct cgcgacccgg
     3301 ttggaatcgc atccgt
//
LOCUS       DATIAL010000414         6280 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1581168, whole genome shotgun sequence.
ACCESSION   DATIAL010000414 DATIAL010000000
VERSION     DATIAL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581168"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /gene="mnmG"
                     /locus_tag="VLA60_12750"
     CDS             complement(<1..1242)
                     /gene="mnmG"
                     /locus_tag="VLA60_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HSF10277.1"
                     /translation="MSKECDIVVVGGGHAGCEAALAAARMGCRVVMLTIDSQKIATMP
                     CNPAVGGIGKGHLVKEIDALGGEIGWNTDQSGIQFRVLNMRKGPAVRATRVQCDKQLY
                     AHRMQETVSRQDNLSIIQGTVDRILTQGGAVNGVMTNSGDTIFTKAVVLTSGTFLKGL
                     MHIGLNHLPGGRAGEPSAENLSDCMRDFGFEIGRLKTGTPPRIDRDSIDFSVMIPQPG
                     DSVARPFSVRTTHIPNPQIHCHITYTNDETHRIIRENLDRSPMYCGIIESTGPRYCPS
                     IEDKVVRFSDKNRHQIFMEPETLESRSFYPNGISTSLPVDVQQAFVRTIPGLEQAVFL
                     RPGYAIEYDYFPPRQLHETLETKLVKGLFHAGQINGTSGYEEAAAQGIMAGINAALQV
                     KGESPLILDRSQAYIGVLIDDL"
     gene            complement(1288..2742)
                     /gene="mnmE"
                     /locus_tag="VLA60_12755"
     CDS             complement(1288..2742)
                     /gene="mnmE"
                     /locus_tag="VLA60_12755"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HSF10278.1"
                     /translation="MGSLDDTICAVATPMGEGGVGIVRVSGTHALPVASSILRLRSGK
                     PLEQIKPYQLYLGQFVWNPRQSGKIVGREIPSVLDEVLVVTMRAPRSYTGEDVVEVHA
                     HGGPVIVNAICEALTQAGARLAEPGEFTKRSFLNGRMDLTQAEGVLDTIRANSLQSLR
                     MAQEHLQGRLSLVIQHQRDRLVKLVAHLEAGMDFVDDDIQFIDQSELKHNTQEVLREI
                     LQLLESAEEGRMIREGIRTVIIGRPNVGKSSLLNGILGTNRAIVSHIPGTTRDVLEEA
                     IMVEGVIIRLFDTAGLRETMDELENEGMNRATQAIEEADVLIMAFDRSSTLNEKDLLF
                     IEKYHEKPCVMVLNKIDLPAKFSHEELQDIVSQHKKISENSSVGEKRYVEVSALSGEG
                     LDNLKKAIKSLAVGRQYEAGDSVLVSRLRHKILLRNAGEALKNALLAIDDGLSAECVA
                     LELRMALNSLGEIVGAITNEDILDQIFREFCIGK"
     gene            complement(2762..4504)
                     /gene="yidC"
                     /locus_tag="VLA60_12760"
     CDS             complement(2762..4504)
                     /gene="yidC"
                     /locus_tag="VLA60_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HSF10279.1"
                     /translation="MEKRVVLFLVLSLGIIFGYDLLLKELGYSPFSTSPIIDQELATS
                     LPKKGADAETTPSSSLSEPPSISSSIPSVEPLLTEEVVVVETPLFRAGISGQGGVITS
                     WELKKYLTQTEENVPVQLVYPNGQFPGPLTVQIKGNEAETQRLRQGNFQVQKNFNELD
                     ESHPTGKVFLTHSSSESDLWIQKELTFHYDSYVVDIIIRTRGISGDIDVLLGTNFGVV
                     EWGQGFIGLLGSAWMVGDELEKLSPDADAPILRRDGPVRWLALQDKYFMSVFIPDNAN
                     GLYSKLETEMVVSAGMSLPGGSGEQVHKTKLYAGPKKYDVLKSFDIGLEDTIDFGWFI
                     YGSWSIVKAVAKPLFSVLRFIYDYTHNYGVAIILLTLGIKLLFVPLQYKSYKSMQGMQ
                     TIQPKVLALQEKFKDNKEKLNQELMKLYKEHKVNPVGGCLPMVLQMPVFVALFNILYM
                     TIDLRQAPFMLWVTDLSAQDPYYVLPVLMGISMVVQQKIMPTTMDPTQAKMMMILPVG
                     LTFLFVTFPAGLVLYWVTNNVLTVTQQFVTDRYILKKPRGGSTDQSPKGSEPALESGK
                     KKRSSSPSGSSSKS"
     gene            complement(4575..4787)
                     /gene="yidD"
                     /locus_tag="VLA60_12765"
     CDS             complement(4575..4787)
                     /gene="yidD"
                     /locus_tag="VLA60_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HSF10280.1"
                     /translation="MIASVLKKIIAVYQYGVSPFMGTCCRFEPTCSHYGFEAIHKHGA
                     MKGVVLMASRILKCHPFHPGGLDPVP"
     gene            complement(4794..5099)
                     /gene="rnpA"
                     /locus_tag="VLA60_12770"
                     /pseudo
     CDS             complement(4794..5099)
                     /gene="rnpA"
                     /locus_tag="VLA60_12770"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            complement(5133..5279)
                     /gene="rpmH"
                     /locus_tag="VLA60_12775"
     CDS             complement(5133..5279)
                     /gene="rpmH"
                     /locus_tag="VLA60_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HSF10281.1"
                     /translation="MSMTYRQPSNLKRKRDHGFRKRMSTKNGRKVLARRRKKGRYRLT
                     VSDE"
     gene            5470..>6280
                     /gene="hisS"
                     /locus_tag="VLA60_12780"
     CDS             5470..>6280
                     /gene="hisS"
                     /locus_tag="VLA60_12780"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HSF10282.1"
                     /translation="MLRSIKGLKDILPQQTPQWRYMETLAHTLAAQSGFSEIRIPIFE
                     STELYARSIGGETDIVEKEMYTFPDRDGSSLTLRPEGTAGVVRAVLEHHLLESPSTRK
                     LYYLGPMFRHERPQAGRYRQFHQFGVEAFGTEDPFMDVEVIALLWRFFCSLGLKDLTL
                     QMNSIGNTQDRENYIRELKQFISSRLEMICGNCQRRYTTNPLRILDCKVPICQEATKD
                     APTLFHHLSPESAVHFQSVLDGLTSIDIPYSLNYRLVRGLDYYSRTTFEITS"
BASE COUNT         1692 a         1585 c         1326 g         1677 t
ORIGIN      
        1 taaatcatca attaaaacac caatatatgc ctgtgaccta tctaaaataa gcggactctc
       61 acccttcacc tgaagggcag cgttaatccc cgccataatt ccctgggcgg cagcttcttc
      121 atagccggat gtcccattta tttgaccggc atgaaagagt cctttgacga gtttcgtttc
      181 caatgtttca tgaagttgtc gtggtggaaa gtaatcatat tcaatcgcat agcctggtct
      241 tagaaatacc gcctgttcaa gacctggtat cgttcgaaca aaggcctgtt gtacgtccac
      301 cggcaagctg gtagaaatcc cattcgggta gaatgagcgt gactcaaggg tttcaggttc
      361 catgaaaatc tgatgtctgt ttttgtcaga aaaccggacc actttatctt caatcgaggg
      421 gcaatagcgc ggccccgttg actcaatgat tccacagtac atcggagatc tatcaagatt
      481 ttcccttata attcggtgag tttcatcatt cgtataggta atatggcaat gaatttgtgg
      541 gttgggaatg tgagtggttc ggacagaaaa tggtcgagcg accgaatccc caggctgcgg
      601 aatcatgacg ctgaaatcaa ttgaatctct atctatcctt ggaggggttc ctgtttttaa
      661 tcgtccaatt tcaaatccaa agtctctcat gcaatccgat agattctccg cagatggctc
      721 accggcccga ccgcccggaa gatgattcaa tccaatatgc ataagacctt tcagaaaggt
      781 ccccgaggtc aggacaaccg ctttcgtgaa aatagtgtcg ccgctattgg tcatgacacc
      841 attgaccgcc ccgccctgcg tcaggatccg atcaactgtg ccttgaataa ttgataaatt
      901 atcctgtctg cttaccgttt cctgcattcg atgggcgtaa agctgtttgt cacattggac
      961 tctcgtggca cgcacggccg gtcctttccg catattgagc acacggaatt gaatgccgga
     1021 ttggtcagtg ttccacccta tttctccacc gagagcatcg atttccttga cgagatggcc
     1081 cttcccaata cctcccacag cgggattaca tggcatcgtg gcgatttttt gggaatcaat
     1141 ggtcagcatc accaccctac atcccatcct ggcggccgcg agagcagcct cacacccggc
     1201 atgacctcct ccaacgacga caatgtcaca ttccttactc attggttcta atccgtattt
     1261 ttatgaacac ttttccaggt atgacattta cttcccaatg caaaactctc gaaaaatttg
     1321 atctaaaata tcctcgttcg tgattgcgcc gacaatttct ccaagagaat tcagtgccat
     1381 tcgtaattct aatgccacac attcggctga aagcccatca tctatggcca ataatgcgtt
     1441 tttcaaggcc tctcccgcat tgcgaagtaa gatcttatgt ctcagccgtg aaacgagcac
     1501 cgaatcaccg gcctcatatt ggcgaccaac agccaggctt ttgatggctt tttttaaatt
     1561 atcaagacct tcaccgctta atgcagagac ttcgacatac cttttctctc ccacagaact
     1621 attttcagaa atttttttat gttggctgac aatatcctgc agttcttcat gagaaaattt
     1681 ggccggcaaa tcgattttat tgagcaccat tacacaaggt ttttcatgat atttttcaat
     1741 aaaaagtaag tctttttcat taagtgttga acttctatca aatgccataa tcaacacgtc
     1801 agcttcttca atagcctgag tggcgcgatt cattccttca ttttctaatt catccatcgt
     1861 ttctcgtaat ccggcagtat caaagagccg tattatgact ccttccacca tgatagcctc
     1921 ttcgagaaca tcacgtgtgg tcccaggaat atgagataca atagctcgat tggttcccag
     1981 tatgccattt aacaaactgg actttcctac attgggtcgc ccaataatta cagtgcgaat
     2041 gccttctcga atcattcggc cttcttcggc ggactccaac aattgcaaga tttctcgcaa
     2101 gacctcctgt gtattatgct tgagctcgct ctggtcaata aactggatat catcatccac
     2161 aaaatccatt ccggcttcca agtgggcaac aagtttcacg agacgatccc gttgatgttg
     2221 gatgaccaaa gatagccgcc cctgtaggtg ctcctgggcc atccttaaac tctgaagaga
     2281 attggcgcgt atggtatcta agaccccttc cgcctgggtt aaatccatcc gcccgttcaa
     2341 aaaactccgc ttcgtaaact ccccaggttc agccaacctg gcgcccgctt gcgttaaggc
     2401 ttcgcaaatg gcgttcacta taaccggtcc tccgtgggca tgcacttcaa ccacatcttc
     2461 tcctgtatag gaccgtgggg ctcgcatcgt cacaaccagg acctcatcca atacagaggg
     2521 aatctctctt cccacaattt tcccactttg acgagggttc caaacaaatt ggcccaggta
     2581 caattggtag ggcttgatct gttcaagagg tttacccgaa cgtaatcgaa gaatacttga
     2641 ggcaacagga agggcgtgag ttccactgac acgaacgatc cccactcccc cttcgcccat
     2701 aggggtggcc acggcacaga tggtgtcgtc taaagagccc atagcttaga cagaggcagg
     2761 attaggattt agaggaagag ccagagggag atgacgaacg tttctttttc ccggattcca
     2821 aagcaggttc gctccccttg ggagattgat ccgtagaccc acctctcggt tttttcaaaa
     2881 tatacctgtc ggtcacaaac tgctgagtca cggtcagcac attattggtt acccaatata
     2941 acaccaaacc tgcgggaaag gtgacaaaca aaaaggtcaa ccctacagga agaatcatca
     3001 tcattttcgc ctgggtgggg tccatcgtgg tgggcatgat tttttgttgg acgaccatag
     3061 aaatccccat cagcacaggc agcacataat acggatcttg ggcagaaagg tctgtaaccc
     3121 acagcataaa cggggcctgg cggagatcga tcgtcatata caaaatatta aagagggcaa
     3181 caaacaccgg catctgcaat accatgggga gacagccacc gactggattc actttatgct
     3241 ctttatagag cttcatcagc tcctggttga gtttttcctt attatcttta aatttctcct
     3301 gaagggccag aaccttgggt tggatggttt gcattccttg cattgactta taacttttgt
     3361 attgcaaggg aacaaataac aacttaatcc ctaatgtcag taaaataatg gccacaccat
     3421 aattatgggt atagtcatag atgaaccgta aaacggaaaa cagcggcttc gccacggctt
     3481 tcacaatgct ccaactgcca taaataaacc atccaaaatc aatggtatcc tccaacccga
     3541 tatcgaaaga cttaaggaca tcgtattttt tgggaccagc gtacaatttt gtcttatgga
     3601 cttgttcccc ggatcccccc ggaagagaca tgccagcaga gacaaccatt tcggtttcaa
     3661 gcttagaata caggccgttc gcattatccg ggataaatac actcataaaa tatttatcct
     3721 gaagggccaa ccacctgaca ggaccatcgc gtcgcaggat tggagcatcc gcatcgggag
     3781 acaatttttc caattcatcc ccgaccatcc aggcagatcc cagtagtcca atgaatccct
     3841 gaccccattc caccacacca aaattggtgc ccaaaagtac atcaatgtca cccgaaatcc
     3901 cacgggtacg aataatgata tccaccacat aggaatcgta atgaaacgtg agttcctttt
     3961 ggatccacaa gtcggattca gaagacgaat gggttaaaaa cactttaccg gtgggatgcg
     4021 attcatctaa ttcgttaaaa ttcttttgga cctgaaagtt cccttgacga agacgctggg
     4081 tctccgcttc attgcctttg atctgcacag tgagtggccc gggaaactgt ccatttggat
     4141 ataccaattg gacgggaaca ttttcctctg tctgagtgag atattttttt aactcccagg
     4201 atgttatgac gcctccctgg ccggaaatac cagctcgaaa aagcggggtc tcaaccacca
     4261 caacctcttc agtaagcaat ggctcaacag aaggaataga ggaggatatg gaagggggtt
     4321 cagataaaga agaagagggg gtggtctcag catccgcccc tttcttcggc aaactggttg
     4381 caagctcttg atcgatgatc ggactggtag agaatggaga atatcccaac tccttcagga
     4441 gcaggtcata cccaaagata atccctaggg aaagaacaag gaaaaggacg acgcgctttt
     4501 ccatttttct aactcgtgag tctcattaaa gctggtggta attaacaatc aaacggaggc
     4561 aaaagattca gtgttcaggg aaccggatcc agccccccag gatggaatgg atgacactta
     4621 agaattcgac tagccattaa gaccacaccc ttcatcgcgc catgtttatg gatggcctca
     4681 aagccataat gggaacacgt cggttcaaaa cggcaacagg ttcccatgaa cggggaaacc
     4741 ccatattggt agactgcaat aatttttttc aaaaccgagg cgatcatttt ttattaaaga
     4801 agccccattt tggctaatgt gcccttccag gtttccccaa tcttctgata cttcacaccc
     4861 aaaaccatgg ttttggggta cacgatgcaa tgaaaaccac cggccattcc agattgcgat
     4921 tccctgacga gttcacgaaa aatccgtttc agccgatttc tcgtgacggc tttgcccact
     4981 cgtcgaccaa cgacgatgcc tatccgagac ggatcatgat tgggtacacg actaacaagg
     5041 agggtaaaaa atgggttccg cacgacagac ccattggcct tcactccatc gtattcatat
     5101 tttttcttta aaaaaaacgg gaagccctcc ccttactcat cagaaaccgt caagcggtat
     5161 ctgcccttct ttcgtcgacg ggcaaggacc tttctcccat tcttagtgct catgcgcttt
     5221 ctaaagccat gatctcgttt tctctttaaa ttagagggct gtcgataggt cattgacacg
     5281 gcattgttct ccttttagcc aatttcttat gaacacgcca ttttatgggg ggaaagagca
     5341 agagtcaaac gaattttctc cctcctaaac tgacacccaa acccattgat tcgttgactg
     5401 gattctggcg gaaagatata gtcggattct cttttcccca atattttctt caacagaata
     5461 aggcttaccg tgttacgctc cattaagggt cttaaggaca ttttgcctca acaaaccccg
     5521 caatggcgct atatggaaac attggcccat acccttgctg cccaatccgg attttctgaa
     5581 atacgtattc cgatattcga gtccactgaa ctctatgcca ggagtattgg aggagaaacc
     5641 gatattgtag agaaagagat gtataccttt cctgatcgag atggaagttc tctgacgtta
     5701 cgtcctgagg gtacggccgg ggtggtgcga gctgtgttgg agcaccatct tttggaatca
     5761 ccctctacac gaaaactata ttatcttggc ccgatgttcc gtcatgaacg accgcaagca
     5821 ggtcgatacc ggcaatttca ccaatttgga gttgaagcgt tcggaaccga agatcccttc
     5881 atggatgtcg aagtgattgc tcttttatgg aggttttttt gttcgctggg acttaaagat
     5941 ctcactttgc aaatgaattc aattggcaat actcaagatc gtgaaaacta tattcgggaa
     6001 ctcaaacaat tcatttcttc tagattagaa atgatttgcg ggaactgcca gcgtcgatat
     6061 acgactaatc ccttacgtat tctagattgt aaggttccga tttgtcagga agcgacaaag
     6121 gacgccccaa cacttttcca ccacctctcc cccgaatcgg ccgttcattt tcaaagcgtc
     6181 ctcgatggac tcacgtccat agacatcccg tattccttga actaccgttt ggtgagaggc
     6241 ctggattatt actcccgcac gacctttgaa atcacttctc
//
LOCUS       DATIAL010000415         6546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1581291, whole genome shotgun sequence.
ACCESSION   DATIAL010000415 DATIAL010000000
VERSION     DATIAL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581291"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VLA60_12785"
     CDS             complement(<1..1463)
                     /locus_tag="VLA60_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HSF10283.1"
                     /translation="MSHLDKTLQVQRSFMPLPSAGFARNVLVGFSVTLITLTLCPQPT
                     LGETSFLPPNDSTEALTFNYAIANSYSRPATSIETQPFETSTNHNNQQSSILIDQTGE
                     TTPGIPGLSTEDDPNQSSAEALAQPPTHQTQSSEAGKPVAPSSVDVSQESNASRDQRI
                     FFSDENKFEALDTQTQFSINASTKLRFYPAESSEQRSQVALARFAKVFDQTSEAPHQR
                     KPGTYGTIPLVLNDSVEKNLEYFQYGIHERFQSYLDRFHHYRDLVEPVFRELGLPPEL
                     MYLSLVESGFNPRAFSRSRASGPWQFMKGTGRVYGLDVDWYLDERRDPIKSTVAAAHH
                     LRDLYDQFGSWPLALGAYNAGSGKISRAIKKTGTRDFWTIRRSRHIRRETKDYVPRFI
                     AATLIAQNPTAYGFTTPDGDRHEFEEVLITKRVHLSAVTKQTGIPVEELQRLNPELRR
                     SIVPNLTAPGYYLKVPLGMASLVEELHPTLAVWTQPPP"
     gene            complement(1675..3624)
                     /locus_tag="VLA60_12790"
     CDS             complement(1675..3624)
                     /locus_tag="VLA60_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HSF10284.1"
                     /translation="MATSPTLIHPVASEAKRTQTAASQGTTDFRSFGTKVALVTMPFS
                     YSKFPSIQLGTLSSLLKAQGIGAQTYHFNLQFAHKIGVPLYEILCEKRGLFGEWLFSA
                     LLFRNNPKHREYPMVFKPIFEDTAREAGCSISHLEEIARSIAPQFLTWCLTSVNWSQF
                     EVVGFTSTFDQNVASLSLAKLIKDVYPDVRIVFGGANFDGEMGLEHFRAFPWIDYVVI
                     GEGEQAFPPLVQRILLDDETDIPPGVAFRREGQIHFTPQETLFTKFSETPPPDYDDYF
                     AELQELAKQGSTGLNRILLYEGSRGCWWGEKHHCTFCGLNAQAMEFRAKNPAQVASEL
                     DYLSSRYDTTRFRLVDNIIDMKYVDGVFGQLAQEHVDLDVFMETKSNLNKRQIQTLAK
                     GGVKCMQPGIESLSPAQLKEMDKGVSPLQNIECLKWSRYYNIDLSWNILLGFPQETDT
                     DYQQQIELIPSLIHLQPPESTAKLWLERFSPYFKWPERYGIRRTGPGLAYAYVYDERQ
                     VDLNKIAYDFEYTIDWKVDPHLYKELVRLVEEWKQRYHSSNRPFLFFGKALSYLTVYD
                     GRGLTPTSERFEGPHAFILEFCNEQPKSVEQIRKALQGCSFSGSEEDIIVPITDILTT
                     LKGKRLLHEERGRYFTLALPVNPHR"
     gene            complement(3633..3977)
                     /locus_tag="VLA60_12795"
     CDS             complement(3633..3977)
                     /locus_tag="VLA60_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HSF10285.1"
                     /translation="MSLADNTCVPCRGGVPPLGAQKIQDLLGQLETGWTLNAQGHIEK
                     TYTFDNFADALAFTNRVGNVAELEGHHPDIYLAWGKCKVEIWTHKIQGLTESDFYLAA
                     KADRAFHLPAAY"
     gene            4705..5265
                     /locus_tag="VLA60_12800"
     CDS             4705..5265
                     /locus_tag="VLA60_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10286.1"
                     /translation="MPVNALRKPGARMSLQEALARVEKLKRLTESSNPHEAALAAMRL
                     KDFDVEVARFPRPEPVAEDVSPPEDHAYLNKVVEILDEMPIVPYETLDEIEVGQSADK
                     SKVNWDELHFALVKKAQRMGADALIHIQLKGTAEQKVLGATALKYLTPKEVLEIQQST
                     ALEDAEKAYNESQKERLDEDLAPGIG"
     gene            5722..6294
                     /locus_tag="VLA60_12805"
     CDS             5722..6294
                     /locus_tag="VLA60_12805"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSF10287.1"
                     /translation="MSKFPTTILGILGITVWLTVMNADLAHTKETELLSVGIRGGLNS
                     NTLGIPPTEKEDFQQYDVFAVVGTPWSWDFPSGWEIRFRLNVAAGALRAAGEAGFIGE
                     VSPGLAFTKPNWNLTFDIGGGGAYLSREKYGRQDIGGPVQIVGHAGITYHLPWNISLG
                     WRFHHISDAALYGSNNRGVDLHLYELSYRF"
BASE COUNT         1714 a         1437 c         1683 g         1712 t
ORIGIN      
        1 ggaggaggtt gggtccagac agcgagtgtc gggtggagtt cctcaacaag cgaggccatt
       61 ccaaggggta ccttcagata ataaccagga gccgtaaggt tgggcacgat actgcgtcga
      121 agttcagggt tcagccgttg gagttcctca accggaatcc cagtttgttt ggtgacggcc
      181 gaaagatgca cgcgttttgt aatcagaact tcttcaaatt catggcgatc accgtccgga
      241 gtcgtgaatc cataggccgt gggattttgg gcaataaggg ttgccgcaat aaagcgaggc
      301 acatagtctt ttgtttcccg tcttatgtgg cgtgaccgtc gaattgtcca aaagtcacgg
      361 gtgcccgtct ttttgatggc ccgcgaaatc tttccgcttc ccgcattata cgcaccaagg
      421 gccagaggcc aggagccaaa ttggtcatat aaatcacgaa gatgatgagc cgcagcaaca
      481 gtggatttaa tcggatcccg ccgttcatcc aaataccagt cgacatcgag gccatacaca
      541 cgacccgtcc ctttcataaa ttgccagggg ccggaagcac gggatcgaga aaatgctcgt
      601 gggttgaacc cactttcaac cagtgaaaga tacatgagtt ccggcgggag gcccagttca
      661 cgaaaaacgg gttccacaag atctcggtaa tgatgaaacc ggtcaagata cgattgaaat
      721 cgttcatgaa ttccatactg gaaatattca agatttttct ctacggaatc gttcaggaca
      781 aggggaatgg tgccatatgt cccgggtttt ctctggtggg gggcttccga tgtttgatcg
      841 aaaactttgg caaatcgagc tagggcaact tgtgaccgtt gctcagatga ttcagcaggg
      901 taaaaacgta atttcgtaga ggcgttaatt gaaaattgag tttgagtgtc tagggcttca
      961 aacttgttct cgtcagaaaa aaaaatccgc tgatcccggg aagcgttaga ttcctgagag
     1021 acatcaacgg atgagggagc aacgggtttt ccagcctccg atgattgggt ctggtgtgtt
     1081 gggggttgag ctagggcttc tgcagaagac tgattcgggt catcttcggt agataaccct
     1141 ggtatgcctg gagttgtttc cccggtttga tctatgagaa tagacgattg ttgattgtta
     1201 tggttggtgc tcgtctcaaa cggttgagtc tcaattgagg tcgctgggcg agagtagcta
     1261 ttcgcaatgg cataattaaa ggtaagagcc tctgtggaat cattgggagg taaaaaagag
     1321 gtttcgccga gggttggttg agggcagaga gttaacgtga tcaaagttac actgaagcca
     1381 accagtacgt tgcgggcaaa accagcagat ggcaaaggca tgaaggagcg ttgaacttgt
     1441 aacgttttat ctaagtggct cattttataa gagttttcgg caaatctgtg cacaacttga
     1501 taggctaccc tcgtaagaaa aaattgtcaa acatattcat gattttttga cattggtcga
     1561 tgggtagaaa tcctgcacga actgcaccaa aaagtaagtg tatgaaaaaa ggagaaaaat
     1621 acggtggcct gtgaatgaga gtggcgtttt tattccagtt gttaggcaaa aatgtcaacg
     1681 atgaggattg accggcaggg ccagagtaaa ataccggccc ctttcctcat gcaatagtct
     1741 tttccccttc aatgttgtga ggatatccgt gatgggcacg ataatgtctt cctccgatcc
     1801 tgaaaaggag caaccctgca aggctttacg aatctgttca acggacttgg gttgctcatt
     1861 gcaaaattct aagatgaagg catggggtcc ttcaaatcgt tcgctcgtcg gagttaagcc
     1921 tctcccatca taaacggtga gatagctcaa ggcttttcca aagaagagaa agggtcgatt
     1981 agaagagtga tatcgctgtt tccactcttc aacaagtctg acgagttcct tatagagatg
     2041 tggatcgact ttccaatcga ttgtgtactc aaaatcataa gcgattttgt tgagatcaac
     2101 ctgccgttcg tcatacacat aggcataggc taaccccggt cctgttcggc gaataccata
     2161 gcgttcaggc catttaaaat aaggactaaa ccgttcgagc cagagtttgg ccgtggattc
     2221 tggaggttgc aggtggatca atgagggaat gagttcgatt tgttgttggt aatcggtatc
     2281 tgtttcctgt ggaaagccca ataaaatatt ccaggaaagg tcaatattgt aatatcggct
     2341 ccattttaag cattcaatgt tttgtagtgg gctgacaccc ttatccattt cctttagttg
     2401 cgccggactc aaactttcga tgcccggttg catgcattta accccaccct tcgccagtgt
     2461 ctgaatttgt cgtttattga gattgctttt cgtctccata aacacgtcca ggtccacgtg
     2521 ttcttgggcc agttgcccga agacaccgtc cacatatttc atatcaataa tattatcgac
     2581 caagcgaaaa cgggtggtgt catagcggct ggaaagatag tccaattctg aagcgacctg
     2641 agcaggattt tttgcgcgga attccatcgc ttgagcattt aatccgcaaa aggtgcaatg
     2701 atgtttttct ccccaccagc acccacgaga tccttcatag agcagaatcc ggttcaatcc
     2761 ggtggacccc tgcttggcta attcctggag ctctgcaaaa taatcgtcgt aatcaggtgg
     2821 gggcgtttct gaaaacttgg tgaataaggt ttcctgaggg gtaaagtgga tctgtccttc
     2881 ccgtcgaaag gccactccgg gaggaatatc cgtttcatcg tccaggagga tgcgttgaac
     2941 caatggagga aaggcttgtt ccccttctcc gatcacgacg taatcaatcc agggaaatgc
     3001 ccgaaaatgt tccagcccca tttctccgtc gaaatttgcc cccccaaata caatccgaac
     3061 atcgggatat acgtccttga tcaatttggc cagactcaag ctggcgacat tttgatcgaa
     3121 cgtggaggtg aagcccacca cctcgaattg actccaattc actgatgtca ggcaccaggt
     3181 taaaaattga ggagcgatcg accgggcaat ctcttctaaa tgactgattg agcagccggc
     3241 ttcccgagcc gtgtcttcga agatgggttt aaacaccata ggatattccc tgtgtttcgg
     3301 attattccga aacaggagtg cagagaacag ccattcgcca aataggccac gtttttcgca
     3361 taagatttcg tataacggta ccccgatttt atgggcgaat tggagattga aatgataggt
     3421 ttgggcacca atcccctgcg cttttagtaa ggacgagagg gtccctaatt ggatcgaggg
     3481 aaatttcgaa taactaaatg gcatggttac cagtgcgacc ttcgttccga aagatcggaa
     3541 atcggtggtg ccctgagagg ccgcagtctg ggttcgtttt gcttctgaag cgaccgggtg
     3601 gatcaaagtc gggctggtgg ccataagagc cttcaatagg cagcaggaag gtgaaaggcg
     3661 cgatcagcct tggcggctaa ataaaaatca ctttcggtga ggccttgaat cttgtgagtc
     3721 caaatttcta ccttgcattt gccccaggcc aaatatatat caggatgatg accctcaagt
     3781 tccgccacat tccctactcg attagtaaaa gccaaggcat ctgcaaagtt gtcaaaagta
     3841 taggttttct caatatgacc ttgagcattg agcgtccatc ccgtttctaa ttgaccaagc
     3901 aggtcctgga ttttttgagc tcccaacgga gggacgcccc cacgacaagg tacgcatgtg
     3961 ttatctgcta gactcatttg cttcctcctt ccattctgaa catttctggt ggatggcctt
     4021 tgtcaccctg ttcttggctt gtgccaggga aaacgggttt tcttcccatc tccttcgtat
     4081 gaattatgtt ggatggtata gggaacttca ggcaatcgtg aggtcatacc atacccctgg
     4141 tcaagccact aattatagac atcaccaagt tcggtgcaca attccagagc ggaagtggga
     4201 gtaagcctat gtgcaacatg gggtatctgt ttagggggtt aggttatttg ggctgatccg
     4261 gtttacagca ccatagtaaa ttgaccccca taagaggctt tcaggggacc ggtgctcaat
     4321 ccacaatggt ttctctaggc aaagaaaaag cctattccca ttgtattcag aagagcatgt
     4381 ttgctgatca atcgaggtgg atcaaggtct tcggtggctt cagcccagcg cctcattatt
     4441 acctcgccat gattggatga ccagccttag ggagagaagg ccctaggggg agtgaacagc
     4501 gtatggagtc gttctttcct tgccaaaaga tcatttgaca acctgaatcc tggacaggta
     4561 gaaaaacact ccaaaagctt aggtccgtct gctattgggc attctcgtcg tcattcagga
     4621 cttaaagcca cgttaatgag cgatttcctt ccttcttgaa atgagcctcg gaaggtctat
     4681 gctacacgat cacggagttg tgcaatgccg gtgaatgctc tgcgaaaacc tggtgcgaga
     4741 atgagtctgc aagaagccct tgcacgagtg gagaaactca aacggttaac agaaagcagc
     4801 aatccccacg aggcggcctt ggccgccatg cgattgaaag attttgatgt ggaggtggcc
     4861 cgatttccac gcccggaacc tgttgctgaa gatgtttccc cgccggagga ccatgcttac
     4921 ctcaataaag ttgttgaaat ccttgatgaa atgcccattg ttccctatga aacgttggat
     4981 gagatagagg taggacaatc tgctgataaa tcgaaagtca attgggatga acttcatttt
     5041 gcccttgtga agaaagcgca gcgtatgggg gcagatgcct tgattcatat tcaattaaag
     5101 ggaactgctg agcaaaaggt gcttggagcc acggcattaa aatatctcac tcccaaagag
     5161 gtcttggaaa ttcaacaatc cacggcctta gaggacgccg agaaagccta taacgagtct
     5221 caaaaagagc ggcttgatga agatctagcc cctggtattg gctaaccaac aaaatttttg
     5281 tcagagtgaa gaagacctgg taggctttta tagaagaaag gggaatacgc aaccacattc
     5341 agattctgga tggaccgcct caaaagaatc ggaaggttca ttgagggcga ttgctgcctc
     5401 cttaaaaacc tacaagggga cgacagacat atgagaatat gatatatttc ccacagtgtt
     5461 taccctctca tcgcaccctt ggcattcttt tcttaattcc ctcttgtaat ttttttctca
     5521 cagacttcgg ctacgagcag agagaaattt cacgtgtgga aagaccgtga aagccgtcgt
     5581 tttggcctca agtggcgaag tgtgaagaaa aagaccatgc agtacttaag tcagggggca
     5641 tgcaggttat tgtcaagtgg tggtgtgatt tgccgttgtc acaactcaac aaaaggacca
     5701 actgtggaga gaagcaaaat tgtgtcaaaa ttcccaacca ctatactcgg tattctgggg
     5761 ataacagtgt ggctcacggt aatgaatgcg gacttggccc acaccaaaga gactgaatta
     5821 ctttccgttg ggatacgggg aggactcaat tctaatacgt taggcattcc gccaactgaa
     5881 aaggaagatt ttcagcagta cgacgtattt gcggttgttg gaactccttg gagttgggat
     5941 tttccttccg gatgggagat acgttttaga ttgaatgtgg cggcaggtgc gttgagggcg
     6001 gcgggagagg ccgggttcat tggagaggtc agtcccgggc tggcctttac aaagccaaat
     6061 tggaacctca ccttcgatat aggcggtggt ggggcatatt taagtaggga aaaatacggt
     6121 cgtcaggata tcggaggacc ggttcaaatt gtggggcatg ctggtattac ctatcacctt
     6181 ccctggaata tatcactggg atggcgattt catcatattt ctgatgcggc actgtacggg
     6241 tctaataatc ggggagtgga tctgcacttg tatgagctca gctatcggtt ctgagggtat
     6301 ttgagaagat tccggccatc atgggggtag cgctccgccg ttaaggtcgt cccgcttcat
     6361 ctcgtttttc tttcaatggc cgaattattc atcatcacac tgatgacggc ttccacactg
     6421 ggagtgttga atactactaa tttccaaggg catcttgacc cacaggaacg ttgcatatca
     6481 gaggcctcgg gctgttcaaa aaagttcgtc cagcaaggcc gcagtcattt ttgcgcgcag
     6541 agcgta
//
LOCUS       DATIAL010000416        10155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_75692, whole genome shotgun sequence.
ACCESSION   DATIAL010000416 DATIAL010000000
VERSION     DATIAL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75692"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VLA60_12810"
     CDS             <1..204
                     /locus_tag="VLA60_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10288.1"
                     /translation="MTAGGALKKTVLDTLDKLGWKPTEGISKETREIEYTDTSNVLGE
                     GGEGRFGKSGDFAMPAKPPKIER"
     gene            complement(264..1946)
                     /locus_tag="VLA60_12815"
     CDS             complement(264..1946)
                     /locus_tag="VLA60_12815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF10289.1"
                     /translation="MIQKAMMPGSKQMYGDNPIAQRKTDLYKQEYVHSFVQKWDALID
                     WEARASSEGDFFIRILKERGVKRVLDVATGTGFHSIRLLRAGFEVTSADGSPEMLAQA
                     FENARRAGFIMRTIHADWRWLSRDIHNKYDAVICLGNSLTHLFSEQDRRKALAEFYTA
                     LRHDGVLILDQRNYDGILDEGFTSKHVYYYCGDNVSAEPEHVDEGLARFKYTFPDGEA
                     FHLNMYPLRKEYTRRLMNEVGFQQVKTYADFQETHKVEDPDFYVHVAEKLYKNEERPT
                     TIMGKFGNLNYSTTVDTARDYYNSSDADRFYATIWGGEDIHIGLYESDKDSIFDASRR
                     TVEKMAASVKGLDATKRVLDIGSGYGGSARHLVRQYGCQVGCLNLSEVQNKRNRALNQ
                     KENISLAINVIDGNFESIPMPDGSVDVVWSQDAILHSGNREKVFQEVHRVLAKGGQFI
                     FTDPMKSATCPPDVLQPILDRIHLDSLGSIEYYRQLAAQLGFQEIQIEDLSENLASHY
                     GRVLQELTAQHHALIQVCTEEYLASMKVGLQHWVQAGKSGHLSWGILHFQRN"
     gene            2414..3901
                     /locus_tag="VLA60_12820"
     CDS             2414..3901
                     /locus_tag="VLA60_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HSF10290.1"
                     /translation="MNPLKSTTLVFNISCAMALLFLLWGAMAPKHLAKITGTFQTALL
                     DSFGWLYVLAATGFLVCAFCLIFSRWGDIPLGPDGAKAEFPLHTWFARLFCAGMGIGL
                     VFWGVAEPTSHFHDPPIGEGGTPQAARLALQYSFFHWGLHPWGIYTMVGMALAYFQFR
                     KGTPGLFSSGCQPVLGRHATGRLGTTVDVIAVFATVFGVATSLGFGAVQISGGLSYVF
                     DMPNTLTTQLTVIAIVTVLFMLSAQTGLQRGIKYLSNLNMILALTLLLFLLFLGPTHF
                     IMTVFPSTFGHYIQNLPTMSLNLAPFRDSTWIHNWTLFYWAWWIAWAPFVGTFIARIS
                     KGRTVRQFVLGVLLVPSLFCAFWFTVFGGTGIALELFQDTPLKAIMETKGIEVLLFTV
                     LEQYPMGTVMSLIAIFLISTFFITSADSATFVLGTLTTNGSLNPPNRIKFTWGIIQSL
                     SAAILLWSGGLKGLQTGAILAAFPFVFVIIILIISLLKSFKEETR"
     gene            4024..4473
                     /locus_tag="VLA60_12825"
     CDS             4024..4473
                     /locus_tag="VLA60_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10291.1"
                     /translation="MKLDSLPKEFTPYKQVEIATNKLIDGVALVSVNGFIPVLIGKGE
                     TPEIWVTIPADQQGKEWRPIVSKNTSLLEVITVRTKEKVIQVAMSDSIVLEVKEITPE
                     SAEITQCNLRPFGINVFLEDDCLKVMTNRYSGNTITGAKIFIDVGKG"
     gene            5118..5483
                     /locus_tag="VLA60_12830"
     CDS             5118..5483
                     /locus_tag="VLA60_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10292.1"
                     /translation="MTELDTSLPHITTTETLVRFYVLLSQYIDRCLDEATKRSLPEGE
                     FQKHLAETHTQVTELLTTNRVVKNKAETEFQRITTLCEQFLKTPNDQTIKSSLQHEHD
                     VLRIKMLALSDLLAVFRSV"
     gene            5628..6725
                     /locus_tag="VLA60_12835"
     CDS             5628..6725
                     /locus_tag="VLA60_12835"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF10293.1"
                     /translation="MIDSVAEHISALDDEDWGVREDAAVALGRLGDPRSVQPLIRALR
                     DSDRAVREAATAALKALGEPAILSLGFCLQDLNPQVQESAACILADIANEQVLDPLLS
                     AALSPNWIVRMSAAKGLSRIQNSQAIDTLILLLQDKVPAVREEAGQAIQAIGNTSIPK
                     LLEKLKDQNWKIRLRAVEALTLLKPLEAVGHLMILVLEDSDTAVRQDAVRALGQIGDP
                     RAIPLLLSSLALETPSLKLPSIEALGQLRSTEAIPMLIALVNSLPKEAYEDRMEGCTD
                     PQYKEDLPPLEAAIRALGKIRDPQAIPALIQALQSTLLRTEAAEALTQFGQTAVNPLL
                     NLLKTTKNDNLRRHVLESLSHLGWRPGQIRI"
     gene            6741..7832
                     /locus_tag="VLA60_12840"
     CDS             6741..7832
                     /locus_tag="VLA60_12840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF10294.1"
                     /translation="MPKETFETIISELDHEEDWRRMRATSTCMKGGPKAVEAIIHAMA
                     TGSSHYKVEAAKMLARLRDPRAGQALAHVLKDEDEQVKQAAVDALEHMAGILDEATAS
                     ALLEYLRDDTLQEKVTALLGVIPTSIGPLSERLKDPDPAIRLRAAEILAHLLDPRSAN
                     AFVDAMSDPDLRDLATDTLRKLGAIRDRVDQTMDDLRAVDESELKEGVRQEAVIQLHR
                     IGRPIVEILIEYLEDDDWVVREAAADTLGKIGDIRAVEPLMIRLRSDKDTGVKEHALK
                     SLGLIGDPRPVELFIEAIPIRPLRILAVEALEKVKDIEVLRPHMELFARLKTDRDGLI
                     SYNSGVILDKLEAATAQMNMAEETWAAKE"
     gene            7847..8617
                     /locus_tag="VLA60_12845"
     CDS             7847..8617
                     /locus_tag="VLA60_12845"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF10295.1"
                     /translation="MTEGNHVDPLLSALRDDNEALRNHAASRLGEAGSEAIPKLINMF
                     LEDDCVVREAATSALVQIGEPAVDPLIEALKDDEEWSVREQAATALGKLRAIKSVEPL
                     VQALRLDKDGGVRTAAIWALERIGHPDAVPALVDALMDATLREDAARVLKKLGDSRAT
                     DALVEGLQASNWIVRRHAAEALGKIGDPRSLDPLIHALADEDWLVRRNAAESLARFKD
                     SRAIDPLVPLLEDENEMVRDTAEGALASLGWTPGSSHS"
     gene            8681..9349
                     /locus_tag="VLA60_12850"
     CDS             8681..9349
                     /locus_tag="VLA60_12850"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF10296.1"
                     /translation="MANEAPAKLISIGPKSGTKKDGFNLVTENVTAINLETKQIEVEL
                     LAYDGKTVLMDVSEEAAEDLQKIKIGDGATLRVVEEDGKRIVKSFRIRSKDPNIQRAD
                     AALQDLTDTHWLNRKHAIEVLGELRVEAAVKPLVEMLSDEVGDVRQRAYEALIKIGAP
                     CVPDVIPLLVSEDDDLRQSATEILRKIGKPAVEPLALALGEADEKLQKRIMKVLDRMG
                     YKRK"
     gene            complement(9504..>10155)
                     /locus_tag="VLA60_12855"
     CDS             complement(9504..>10155)
                     /locus_tag="VLA60_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HSF10297.1"
                     /translation="CERVGADVQMVAKGMGLDKRIGSKFLHAGPGYGGSCFGKDTAAL
                     INIGNENDYEMTIAKATKRVNDDQRVRMMEKIREAVGELRGKTIAILGLAFKPNTDDI
                     RDSPALFLAEQIMKAGGSVRTYDPEALEISMKVLPTMVPCQDAYHTMEGADAVVLVTE
                     WNQFRNLDFDRVRAAVNSRIFIDLRNVYEPQRMEDQGFYYLSVGRKTVGTHPSQKS"
BASE COUNT         2555 a         2711 c         2363 g         2526 t
ORIGIN      
        1 atgacggcag gtggtgccct aaaaaaaaca gtcttagaca cccttgataa actcgggtgg
       61 aaaccaactg aaggcatcag caaagagacc agagagattg aatataccga cacctctaat
      121 gtgttaggtg agggaggaga aggccgcttt ggcaagagtg gtgactttgc tatgccggcc
      181 aaaccaccaa aaatcgaaag gtaatataca ccaaatcgcg tggcggggag tggcgcgtac
      241 gctggcggaa gttctttcct ctgttagttt cgctgaaagt gcaaaattcc ccaactgaga
      301 tggccacttt tcccagcctg aacccaatgc tgaagtccca ccttcataga agccagatat
      361 tcttccgtac aaacctgaat tagcgcatgg tgttgtgccg ttaattcctg aagtactcgt
      421 ccataatgcg aggcaagatt ttcagaaaga tcctcaatct gaatttcttg aaaccctaat
      481 tgggcggcca gctgccgata atattcaatc gaccccagcg aatccaggtg aatacgatct
      541 aaaatcggtt gcagcacatc cgggggacaa gtcgcactct tcatcggatc ggtaaaaata
      601 aattgtccgc ccttggccaa cacccgatga acctcttgaa acaccttttc ccgatttccg
      661 ctatgtaata tggcatcctg cgaccacacc acatcaacgc tcccatccgg catgggaatc
      721 gactcaaagt tcccgtcaat gacattaatc gctaaactga tattctcttt ctgattcagt
      781 gcccgattcc gtttattttg gacttcactc aaattcaggc atccaacctg gcatccgtat
      841 tgtctcacga gatgacgcgc tgagcctccg taccccgacc ctatatccaa cacccgtttc
      901 gtggcatcga ggcccttgac agaagccgcc attttctcca cggttcgtcg gctggcatcg
      961 aaaatcgaat ccttatcgga ttcgtacaat ccaatgtgga tatcttctcc accccagatc
     1021 gtggcataaa accggtcagc atccgaactg ttgtaatagt ctcgagccgt atccacagtg
     1081 gtggaataat tcagattccc aaatttgccc atgatcgtgg tcggacgttc ctcattttta
     1141 taaagtttct ccgccacatg tacataaaaa tcagggtcct ccaccttatg tgtttcttga
     1201 aagtctgcat aggtttttac ctgctgaaaa ccaacctcat tcatgagccg ccgtgtatat
     1261 tctttccgta gtggatacat atttaaatga aaggcttctc catcgggaaa cgtatatttg
     1321 aaccttgcga gtccttcatc aacgtgctcc ggttctgcac tgacattgtc tccgcaatag
     1381 taatacacat gcttggacgt aaatccctca tctaaaattc catcgtaatt cctttggtcc
     1441 agaatcagca ctccatcgtg cctcagggcg gtgtaaaatt cagccaaggc ttttctccga
     1501 tcctgttcgg aaaagagatg agtcagggag ttccccaggc aaatcaccgc atcgtattta
     1561 ttgtggatat cccggcttaa ccagcgccaa tcagcatgaa tagttcgcat gataaacccg
     1621 gctcgtcggg cattttcaaa tgcctgcgct aacatttcag gactgccgtc cgcactcgtg
     1681 acttcgaacc cggcacgcaa gagccgaata gaatgaaacc ccgttcccgt cgcgacatcc
     1741 aaaacgcgct tgacgccccg ttccttcaaa atccggataa aaaagtcccc ttcactcgag
     1801 gccctggcct cccaatcaat caaggcgtcc catttttgca caaaactatg aacatactcc
     1861 tgtttgtata aatctgtttt tcgttgggct atgggattat ccccatacat ttgcttacta
     1921 ccgggcatca tcgctttctg aatcattata gacacacctc ctagagggct gttgccctca
     1981 ccaccattag aataatgggt gcaagtgaaa aacggtctgg cgatttcctc tttccataca
     2041 gtattcaatt gccaaaaccc gcaaggacaa aaccgtagtc tgtctcaacg gaccagaaat
     2101 aaaaaaggcc tccgaacttc tcctgcgagc gtaaggtagg cccgactgtt acgtgttagg
     2161 ctttcaatct aaccggttag gctcttcttc gcaacccctt ttgactacgt ccccattttt
     2221 atcaaaacta aaatgatcaa aaccacagac agatttaaaa tctttattga agaaaaaggt
     2281 ggtctaccct taccaatttc gaaatatgtc cattcattgt caacgttttc ggtttatcat
     2341 gatctggggt ttccttgttc ggcaacgcca aatatggtaa ggcctaccct tcctcattcc
     2401 attcaattac tccatgaatc cactgaaatc gaccaccctt gtcttcaaca tctcctgcgc
     2461 catggccctt ctctttctcc tttggggcgc catggccccg aagcatttag cgaaaataac
     2521 cgggacattc caaacagcac ttcttgattc gtttggctgg ttatatgttc tggcagcaac
     2581 aggctttctt gtttgtgcgt tctgtctgat tttttctcga tggggtgata ttccgctggg
     2641 gccggatgga gccaaggctg aatttcctct ccacacctgg tttgccaggc tgttttgcgc
     2701 aggcatgggc attgggctcg tattctgggg agttgccgaa cccacgtccc attttcatga
     2761 tccgcccatc ggcgagggag gaaccccgca agcagcccgc ttggcactgc aatattcctt
     2821 tttccattgg ggccttcatc catggggcat ttacaccatg gtgggcatgg ccctggccta
     2881 tttccaattt cgaaaaggca caccaggact gtttagctca ggttgccagc cggttctcgg
     2941 caggcatgcc actggacggc tgggaaccac cgtggatgtc atcgccgtct ttgccaccgt
     3001 cttcggtgtg gcaacctcac tggggtttgg agcggtgcaa atcagcggtg gattatccta
     3061 tgtttttgac atgccaaata ccctcactac tcaattaacc gtcatcgcca ttgtgaccgt
     3121 gctcttcatg ctctccgctc aaaccggact ccaacgaggc atcaaatacc ttagtaattt
     3181 aaatatgata ttggcgctga ctctgctgtt atttttgcta tttctcggcc cgacccactt
     3241 catcatgacc gtatttccct caaccttcgg tcactacatc caaaaccttc caacaatgag
     3301 cctgaatctg gcgccattcc gagattcgac ctggattcac aactggacat tattttattg
     3361 ggcatggtgg atcgcctggg cgccatttgt gggcaccttc atcgcgagga tttcaaaagg
     3421 gcggactgtg cgtcaattcg tgttgggcgt gttgcttgtt ccgtcactct tttgtgcatt
     3481 ttggttcacg gtttttggcg gaacgggtat cgcattagaa ttgttccagg acacgcccct
     3541 aaaagccatc atggaaacga agggcatcga ggtcctgcta tttacggtat tagagcaata
     3601 tccaatgggc acggttatgt cgctcatcgc catttttttg attagcacct ttttcatcac
     3661 ctcagcggat tcggccacct tcgtgctagg gacactcacc accaacggta gccttaatcc
     3721 acccaaccgg atcaaattca cctggggaat tattcaatct ctgtcggccg ccatcctcct
     3781 gtggtccggc ggcctcaaag gtctgcaaac cggcgctatc ctcgccgcct ttccgtttgt
     3841 ctttgtcatc attatcctca tcatctccct tctcaaatcc ttcaaggagg aaacaagata
     3901 ggctcgacgc gacaaggacc atcatctctc aatggttgat ttgatcctaa aaaatcgcta
     3961 ttactgctat tctcttcaac cccataaaaa tatacctgtc acggagttat tagagaggaa
     4021 tcaatgaagt tagattcgct tccaaaagag tttacacctt acaaacaggt agaaattgcc
     4081 acgaacaaat taatagatgg tgtggcattg gtatccgtaa atggatttat tccagtttta
     4141 attggaaaag gtgaaactcc cgagatttgg gtgaccattc ctgccgatca acaaggtaag
     4201 gaatggcgcc caattgtgag taaaaacacg tctttgcttg aggtgattac ggtcagaacc
     4261 aaggaaaagg tgatccaagt agccatgtcg gacagcattg ttcttgaagt caaagaaatc
     4321 acacccgaga gtgcggaaat tactcaatgt aaccttcggc catttggcat aaatgtgttt
     4381 ttagaagatg actgcctgaa agtcatgact aaccggtatt caggtaacac cataaccggg
     4441 gcaaaaatat ttattgatgt tgggaaagga taaccacggc aagattaatc gagatgccct
     4501 cgaaggaccg cacatatttt tttggtcaag gctcatcgtg aaccaaaatt cattgatggt
     4561 gctattggat tgctcctgtg gtcaggcggc ctcaaagacc tgcaagggtg cgctatcctg
     4621 gcccccgctc cctttttgtc tttgccacta tctttctcat gatctccctc tcaaattttt
     4681 ccatgtgaaa acccacgggt agccaacgct tgacctggac gatctgggtt tcaaattatt
     4741 tccatgcatg gttttcctgc cttcccctct gaaagcatag ggttttcccc tccacaacaa
     4801 tgaaggcgcc cttcgtaacg cggcaagcca tacttcgcct gccttggaga aacccgccaa
     4861 cacaaaaatt ctcggagagg ttggaataag tgcaatgcat tctccggaca gctactttgt
     4921 tctgacaaat cttaagatga attcccgatc cacatcggaa atctataagg ccagaaacat
     4981 aaattctcta ttactttctc attctggact tgcaccatct cccctttatt gacagaatag
     5041 ctctagaacg cccccttgga agggtccaat gaatcctact tgactcaagt aagcggcaaa
     5101 atctgaaagg aacaatcatg actgaacttg acacgagtct tccacatatc accacaaccg
     5161 aaacattggt gagattttat gtcctcctct cccaatacat tgatcgatgc ctggatgagg
     5221 ccaccaaacg ctcactcccg gaaggagaat ttcaaaaaca tctagccgag actcacactc
     5281 aggtaacgga gcttcttacg accaaccggg tggtcaaaaa caaagcggag acggaatttc
     5341 aacgaatcac aaccctctgt gaacaatttt tgaaaacccc gaacgatcag accattaaaa
     5401 gtagtttgca acacgagcat gatgttctga gaataaaaat gttagcgttg agcgacctcc
     5461 tagcggtgtt tcgatcggtg tagcgtctcg tctcccacca cccgcaacag acgaccttcc
     5521 tcctgactca ttgaccgcct aaaaaatcgt gtggtaacgt cgatcttcgg gttttcccta
     5581 atggtagggg aacaggtgaa ttccaaattt ctccaatcgg atcagacatg atcgactcgg
     5641 tcgctgaaca tatttctgcc ctggacgacg aggattgggg ggtacgcgaa gacgccgcgg
     5701 tagccctcgg acgtcttggc gatccccgca gcgttcaacc attgattcgg gcgcttcgtg
     5761 actcggatcg ggccgtgcgg gaggccgcaa ctgcagccct aaaagcgctt ggcgaaccgg
     5821 ctattctctc attaggtttt tgccttcagg atctgaatcc tcaggttcag gaatcggccg
     5881 cgtgcatcct ggccgatatt gccaacgaac aggtactgga tcccttgttg tccgcggcat
     5941 taagtccaaa ctggattgtg cgcatgtcgg cggccaaagg cctgagtcgc attcagaact
     6001 cccaagccat tgatacatta attttgctgc tccaggataa agtgccggcc gtgcgagagg
     6061 aagcgggaca ggccatacag gccatcggca ataccagcat tccaaaattg ctggaaaaat
     6121 tgaaggatca gaattggaaa atccggttgc gggcggtgga agcgttaacc ctgttgaaac
     6181 ccctggaagc ggtaggacat ctgatgattc tggttctcga agattccgat acagccgtgc
     6241 gccaagacgc cgttcgagca ctcggacaga ttggcgatcc tcgtgccatt cccttattgc
     6301 tatcctccct tgccctcgaa acgccatcac tcaaattgcc ctcgattgaa gcacttggtc
     6361 agctcagatc caccgaagcc attcccatgt taatcgcgtt ggtcaactcg ttacccaagg
     6421 aagcttacga agatcgcatg gaaggatgca ccgaccctca atataaagaa gatctccctc
     6481 ccctggaagc cgccatcagg gccctgggaa agattcgcga cccacaggct atccccgccc
     6541 ttattcaagc gctgcagagt acgctcctgc ggacagaagc cgccgaggcc ctcacgcagt
     6601 ttggccaaac agccgtgaat ccattgttaa atctcttaaa aacgaccaag aatgacaatc
     6661 tgcgtcgtca tgtgttggaa tccctttctc accttggctg gaggccggga cagatacgca
     6721 tctaactgaa agacctgacg atgccgaaag aaacattcga aaccatcatt tctgaactgg
     6781 atcatgagga agattggcgc cgaatgcggg ccacctccac ctgcatgaaa ggtggaccca
     6841 aggccgtcga ggccatcatt cacgccatgg cgacaggctc ttctcactac aaagtggaag
     6901 cggcgaaaat gttagcccgc ctacgggatc cccgagccgg gcaggctctc gctcatgtcc
     6961 tgaaagatga ggatgaacag gtgaaacagg cagccgtcga tgccttggag catatggctg
     7021 gcattttgga tgaagccacc gcgtctgcac tcttagagta tttacgcgac gatactctcc
     7081 aggaaaaagt cacagcccta ctcggtgtga ttcccacttc tatcggccct ctgtcggaac
     7141 gactcaaaga tcccgatccg gctatccgtc tccgggccgc agagattctc gcccacctcc
     7201 ttgatcctcg atcggcaaat gcgtttgttg atgccatgtc cgaccccgat ctccgtgacc
     7261 tagccaccga taccctgcgc aaattgggtg cgattcggga ccgtgtcgat caaacgatgg
     7321 acgacctgcg ggcggtggac gaatccgagt tgaaagaagg cgtccggcag gaagcggtta
     7381 ttcaactgca tcggattgga cggccaatcg ttgaaattct cattgaatac ctggaagacg
     7441 atgactgggt ggttcgggaa gccgccgctg acacccttgg caaaattggc gatattcgcg
     7501 cggtggaacc gctcatgatc cggctccggt cggacaaaga cacgggggtg aaagaacatg
     7561 ccctgaaatc cctggggctc atcggcgatc cacggccggt agaattattc attgaagcca
     7621 ttcccattcg ccctcttcgc atactcgctg tcgaagccct ggagaaagtg aaagatatcg
     7681 aagtcctgag accccacatg gaattatttg cccgattaaa aaccgaccgg gacggcttga
     7741 tttcctataa ttccggggtc atccttgaca aactcgaggc tgccactgca caaatgaaca
     7801 tggctgaaga gacctgggcc gcaaaggaat aaaggagtga tgacccatga cggaaggtaa
     7861 ccacgtagac cccttattgt cggcgcttcg cgatgacaat gaagcccttc gaaatcatgc
     7921 cgcttctaga ctcggagaag ccggctcaga agcaattccc aagctgatta atatgtttct
     7981 ggaagacgat tgtgtggtgc gtgaggcagc cacaagtgcg ttggttcaaa ttggggaacc
     8041 ggcggttgat ccactgatcg aggcgttaaa agatgatgag gaatggtctg ttcgggaaca
     8101 ggcagccacc gctctcggga aactccgtgc aattaaatcg gtggaacctt tggtacaagc
     8161 cctccgtctt gacaaggatg gaggcgtgcg cactgccgcc atctgggcct tagaacgcat
     8221 tggtcatcct gatgccgttc ccgccttggt ggatgccttg atggacgcga ctcttcgtga
     8281 ggatgccgca agagtgttaa aaaaactggg agattcccgc gcgactgatg ctctggtgga
     8341 gggcttacag gcctcgaact ggattgtgcg ccggcacgcg gcagaggcgt tagggaaaat
     8401 tggcgatcct cgcagcttag atcctttaat tcatgctctg gccgatgagg actggttggt
     8461 ccgtcgtaat gccgcagaat cattggctcg cttcaaggat agtcgagcca tcgatccctt
     8521 ggtccccttg ctcgaagatg aaaatgaaat ggttcgagac actgccgaag gtgctctggc
     8581 aagcttggga tggacacccg gttcttccca ttcatagtat acatagcaat caatgctctt
     8641 tccgcttttt tttaactatc actaaggaca ccgacacatt atggctaatg aagcacccgc
     8701 aaaactcatt tcgattgggc ccaagagtgg cacgaaaaaa gatgggttta atctggtgac
     8761 cgaaaacgtc actgcgatca atcttgagac aaaacaaatt gaggtcgaac tcctggcata
     8821 cgatgggaag accgttctta tggatgtcag cgaagaggct gccgaagacc tccaaaagat
     8881 caaaattggt gacggggcga ctcttcgcgt cgtggaagaa gacggcaagc gaatcgtcaa
     8941 aagttttcgc atccgctcta aggaccccaa tattcaacga gccgatgctg cgctgcagga
     9001 tcttaccgat acgcactggc tcaatcgcaa gcatgccatt gaagtccttg gcgaactccg
     9061 ggtggaagca gccgtgaaac cgttggtgga aatgctcagt gatgaagtcg gtgacgtccg
     9121 gcagcgtgcc tatgaggcct tgatcaaaat aggagcaccc tgtgtccctg atgtcatacc
     9181 tcttttggtc tccgaagatg atgaccttcg acaatcggca accgaaattc ttcgaaagat
     9241 tggaaaaccc gcagtggagc ctttggctct ggcattaggc gaggctgatg aaaaacttca
     9301 aaaacgcatt atgaaggtgt tggaccgcat gggctataaa cgcaaatagg gtactcggca
     9361 gggtacacca aaagccgacc atccgctcgc atacaagacc cagaaggcaa cttgacgcgc
     9421 ctttttcgct aatcctgact gttctctgga cagacttgcc aatggaagct aggcttcgag
     9481 caagagccgt gtcttgcgat ctcttagctt ttttgggagg gatgggttcc cacagttttt
     9541 cgacccaccg aaagataata aaatccctga tcttccatcc gttgcggttc atagacattg
     9601 cgaagatcga taaaaatccg ggagttcacc gcagcccgaa cccggtcaaa atccaaattt
     9661 cgaaactggt tccattccgt gaccaacacc acggcatccg ccccttccat cgtgtgatag
     9721 gcatcctgac aggggaccat ggttggtaac accttcatcg atatctccaa ggcctccgga
     9781 tcgtaggtcc gcacgctccc gcccgctttc atgatctgct ctgcaaggaa caaggccggt
     9841 gaatcccgaa tatcgtccgt gttgggttta aaagccaatc cgagaatggc aatggttttt
     9901 ccgcgtaact ccccgaccgc ttctcgaatt ttctccatca tccggactcg ctgatcgtcg
     9961 ttcacgcgtt tggtggcctt ggctatggtc atttcatagt cattttcgtt tccgatattg
    10021 atcagtgcgg cagtatcttt tccaaaacac gaccctccat atcctggacc cgcatgcaga
    10081 aatttcgacc cgattcgttt atccaatccc atccctttgg ccaccatttg cacatcggct
    10141 ccaacccgtt cgcag
//
LOCUS       DATIAL010000417         9240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1474369, whole genome shotgun sequence.
ACCESSION   DATIAL010000417 DATIAL010000000
VERSION     DATIAL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474369"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(679..813)
                     /gene="tatA"
                     /locus_tag="VLA60_12860"
                     /pseudo
     CDS             complement(679..813)
                     /gene="tatA"
                     /locus_tag="VLA60_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(932..1237)
                     /gene="tatA"
                     /locus_tag="VLA60_12865"
     CDS             complement(932..1237)
                     /gene="tatA"
                     /locus_tag="VLA60_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HSF10298.1"
                     /translation="MFGSFGWMELMLILIIVLIIFGAGKIPQLGEGLGKAIKGFKKSV
                     AEADALDVTPNEQGEGGQAPPPQPEQLASQHQTAEVPPPPPAQGVPQPTESTQSKQG"
     gene            complement(1498..2934)
                     /locus_tag="VLA60_12870"
     CDS             complement(1498..2934)
                     /locus_tag="VLA60_12870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HSF10299.1"
                     /translation="MSASPSMDPDALPQLVGDFQPVDLWQAHINRLFYGLRGSRVREY
                     YQTVAAADFRLGFALAEDYWRRCGQRAKTQGADQGTTPLTVMEWGAGNGNLAACFLDR
                     LQELDQEKRIFPRITYRCIEREPVLLEQAKANTDLAKHRDRVTFDLVSIEDLSDFPDG
                     SIDRIICNELWSELPTKLILRKGGEIHEEQLRPNLNEKRLADFPDWPKFIEAFGQQDI
                     ESLTALPSFLEDLVWEREYRPIETKDFPFRRTVVEFLKQIDEEVLVPYNVGACQSLKE
                     AKRLLAPNAIGFSGFDAGTVDPQVLNDPEKPCYTVQGGQFSFMVNFQLMQDVARHLNI
                     HTGVIESQRDFVGRSLSTNVITVMDLLASHPSPPEGQAWQLDALILRTLEALNRTYRS
                     PYHRHIEFPLSESTPAHERAGLENLVQSLPPHGVPDTIAYLTESEIWKAMPDLEKLGY
                     DSEGIKGMLELPLQPVDYIHMFFALKDS"
     gene            3175..3846
                     /locus_tag="VLA60_12875"
     CDS             3175..3846
                     /locus_tag="VLA60_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10300.1"
                     /translation="MTHPLPVMPRSHLLGILMSILLFTMLSTAWGGDSPVQTDPPSVF
                     QPKDTQHGFSQYDTPPDHFSDAPSLYDNYSQSPLIPSLDKKPGGVFLGLDFAESFEVK
                     PTTRGGHTHIPVRPTETFPPDVTSVYLVFTVHEHLSSYQIIGRLFSELETGMHAPQWL
                     DEDIVDLATEDESGFLKFFPPDGTWRPGQYRVEIFVGYIANDVNKLGTMRFTIGPHPA
                     APALP"
     gene            4046..4309
                     /locus_tag="VLA60_12880"
     CDS             4046..4309
                     /locus_tag="VLA60_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HSF10301.1"
                     /translation="MLKKSGSYLLLFIFIGGLLGSILGEILQVVAPQGTVQNIFVQAL
                     NLGLDPPVTVNLVLIKFTLGFLLKMNLLTVLGMFLGAYVYKHI"
     gene            complement(4512..6521)
                     /gene="uvrB"
                     /locus_tag="VLA60_12885"
     CDS             complement(4512..6521)
                     /gene="uvrB"
                     /locus_tag="VLA60_12885"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HSF10302.1"
                     /translation="MMQATNTFSLKSDYTPKGDQGNAIAALTDGLEQGLKHQVLLGVT
                     GSGKTFTMANVIVNVQKPTLVVVHNKTLAAQLYQEFKSFFPDNAVEYFVSYYDYYQPE
                     AYIPTTDTYIAKDSAINDTIDQMRHAATTALLQRNDIIIVASVSCIYGLGSPEVYHDM
                     LLYLEPGMEMRRERILAKLVDIQYSRNDLELQRGMFRARGDVIEIYPASSESNTVRVE
                     LFGDEVEALYEIDPLTGQSLRKLPKIAIYPKSHYVIAPDRYEQAITGIESELEERIAY
                     FRRTNQLLEAQRIEQRTRFDLEMIRTMGYCHGIENYSRHLSGRAPGEPPPTLLDYFPK
                     DFLLIVDESHATVPQFGGMYEGDRSRKNTLVEYGFRLPSAMDNRPLKFPEFERCMNQV
                     VYVSATPGPYELKHAGKALVEQIIRPTGLIDPVMDVKPAKGQVEQLLGEIRLRVAKGQ
                     RVLVTTLTKRMAEDLTEYYHDQGLKVRYLHSDIKTLERAEIIRDLRKGVFDVLVGINL
                     LREGLDLPEVSLVAVLDADKEGFLRGYRALVQTAGRAARHREGTVILYGDVVTDSMRR
                     ALDETGRRRTIQLAYNAEHGITPETIKKRIHDLEYQLAEADYVDVSLAAEAVAAYGGE
                     KDLEQNIAALEKDMKAAAKALEFERAAQLRDTIRVLRQKQIQVGI"
     gene            6654..7094
                     /locus_tag="VLA60_12890"
     CDS             6654..7094
                     /locus_tag="VLA60_12890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF10303.1"
                     /translation="MATTTIAEFMGHDHDRLDEIFNRFQAFKKQDSQKARLLFQELLT
                     GLHRHIRWEEEILFPFFEAQAEMVRDHGPTGVMRNEHRQIKDFLEQIQAKVAAGDLST
                     EEAEEGLLQVLTAHNVKEEKILYPWIDNCVSDEERASLLARMGN"
     gene            complement(7145..8827)
                     /locus_tag="VLA60_12895"
     CDS             complement(7145..8827)
                     /locus_tag="VLA60_12895"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HSF10304.1"
                     /translation="MSHSSINLDIANRIPLQPIAEVAREKLQLDASQIELYGRYKAKI
                     PLASLPPFPNKPESKLILVTAMSPTTAGEGKTTTLIGLVDGLNKIGKRAVGAVREPSL
                     GPCFGMKGGGCGGGYAQVVPMTDINLHFTGDFHAITSAHNMLAALVDNHLYWGHKPHI
                     DPRRVTWGRVLDVNDRSLRSVVTGLGGKSNGFTREGRFDITAASEVMAILCLATDLQD
                     LQQRLGHIKVGRTVNKEMVFAKDIQAEGAMTVLLKDALNPNLVQTLEHNPALVHGGPF
                     GNIAHGCSSVVATKAALTLADYVVTEGGFGADLGAEKFFNIKCRRSGLTPACAVVVGT
                     IKALKLHGGAKEKDLEQEDLDALRHGLPNLLRHLENVKKFGVPTVVAINQFTSDTKRE
                     LELVEQACADHGVKMALANHWAEGGAGAVCLAETVRDVIEKEPADFRVLYPDTMPLAE
                     KVRIIAREIYRAEDVEIPPAVHARFAELEKNGFGHFPICMAKTQYSFSTDPAKKGAPT
                     GFTLPVREVRLAMGAGFVVVITGDMMTMPGLPRVPAAQSIGIDAQGNIVGLF"
     gene            complement(9027..>9240)
                     /locus_tag="VLA60_12900"
     CDS             complement(9027..>9240)
                     /locus_tag="VLA60_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10305.1"
                     /translation="KILGEGDGPATHGEPEYKAECREKFMHVLYLTKLGVEYQAGPGC
                     LLCRNRPEADLPSRFLQVKSHHVHRE"
BASE COUNT         2202 a         2599 c         2146 g         2293 t
ORIGIN      
        1 tggtaaagga tcggtaaaaa gtcctcattg cactcctcct gttggtgaat agccctgtgg
       61 attcaagatc caacgccctg taccccattt actaccatcc agagctgctg gtctctctca
      121 tccgcttcct tcttgactgc gtgattcttg ccgtctcaag cactaccatg ttttccatta
      181 accaaaccaa tagttccctg ttctacgact cttccgatct attattgttt gccatttaga
      241 tgccaagtcc cattctttgt cgtaaatcat taaaaagtaa tgtctttttc ctgactttct
      301 cgctgatttt tgttcatgac cctatcgctt gaatgctacg atagttgcaa aaaccacacg
      361 acaaaagata accgtagacc cgcctgcagt gtgcacaatg aacagtctga tgaccgtccg
      421 gaaggatttc gtgatgacag ataaatttta aaaatcgaaa gaaggcttgg gtgtcaacga
      481 aacgatccag cagtcaacgt cctcacggag tcgcacctcc cctggctacc ccattttgga
      541 tccaccggac tcggtggctt gttctgtcgc ccctttgatc tctgcttcgg cttctttgat
      601 tgacgattcc aaatcttgtt cgacagattt gaattcctgc gtaatcatat tgatctccgg
      661 ttcaaccgac tgccgaagat cttccgttgt ttccttaaac cctttgaccg ctctcccgac
      721 ctgcttgccg atttccggta attccttagg accaaagagc aggaacgcga tgacgaggat
      781 gatcaaaatt tccatcgctc ctaagccgaa catcttcgca ctcctctata gtctgcccgc
      841 tgaatatata ccttgagcgt cgatcaggct taggtccatg ctgcctgtcc cttcggtggg
      901 cggtcttcac cgtgtcaatc tcacccgcga attatccctg tttggattgc gtggattccg
      961 ttggctgcgg caccccttgc gccggtggcg gaggaggaac ctctgctgtc tggtgttggc
     1021 tcgccaattg ttccggttgt ggcggtgggg cctgcccgcc ttcaccttgc tcattaggcg
     1081 tcacatccag cgcatccgct tcggcaacag actttttaaa gcccttgatc gctttgccta
     1141 agccctcacc aagctgcggg attttccctg caccgaaaat gatgagcaca atgatgagga
     1201 tgagcataag ctccatccat ccaaacgatc caaacacagc tacctcgtct tattgtgaat
     1261 gagtgtttac gttatttacg ttcttgggaa aatccgccgc agtatacaca acccttttca
     1321 aaaaatctac ctgattgcaa atttcttgca tcggcaataa ctttttttcc gattgagatt
     1381 ttgccttcac aggatccact cctccgttga gagacttcca attacacaat catgatgccc
     1441 gcgttccgcc ctaatcaatg gcacacgaca aaacgcatcc cgcaactgtc aggatgacta
     1501 tgaatccttc aaggcaaaaa acatgtggat gtaatccacc ggctgtaggg gaagttccaa
     1561 catgcctttg atgccttccg aatcgtatcc caatttttcc aaatcgggca tggccttcca
     1621 aatttccgat tcggtgagat aggcgatggt atccggcaca ccatgtggcg gtaacgattg
     1681 caccagattt tctagtccgg cgcgttcatg agcgggagta ctttcggaaa gcggaaattc
     1741 gatatgacga tggtagggac tccggtacgt gcggttcaat gcttccaggg ttcgaagaat
     1801 caacgcgtcc aattgccagg cctggccttc cggcggcgaa ggatgtgatg ccagcagatc
     1861 catgacagtg attacattgg ttgacaagct ccgtcccaca aagtcccgtt gtgattcaat
     1921 cacgcccgtg tgaatgttca aatgcctggc cacgtcttgc atcaactgaa agttcaccat
     1981 aaagctgaat tggccaccct gcaccgtata acagggcttt tccgggtcat taagcacctg
     2041 cggatcgacc gtccctgcgt caaaaccgct aaatccgatc gcgttcggag ccaacaatcg
     2101 tttggcttcc ttgaggctct gacacgcgcc cacgttgtaa ggcacgagga cttcttcgtc
     2161 gatctgtttg agaaactcca cgaccgtgcg gcgaaatgga aagtccttgg tctcgatggg
     2221 gcgatattcc cgctcccaga cgagatcttc gagaaacgag ggtaatgcgg tgagagattc
     2281 gatgtcctgc tgaccaaagg cctcaataaa ctttggccaa tccgggaaat ccgccagccg
     2341 cttttcgttt agattgggac gaagctgttc ctcatggatc tcaccgcctt ttcgcagaat
     2401 gagcttggtt ggcaactcgc tccagagttc attgcagata atccgatcaa tactcccatc
     2461 cggaaagtcc gacagatcct cgatagacac aaggtcaaac gtgacccggt cacgatgctt
     2521 agccagatcc gtgttggctt tagcttgctc caacaagacc ggttcgcgct ctatacaacg
     2581 ataggtgatt ctcggaaata tccgtttttc ttgatccaac tcctgaagac gatccaagaa
     2641 acaagccgcg agattcccat tcccggcgcc ccattccatc acggtcaatg gtgtggtccc
     2701 ttgatcggca ccttgagttt tcgctcgttg tccacaccga cgccaatagt cctcggccaa
     2761 ggcaaagcct aaccggaaat cggccgcggc caccgtttgg taatattctc gaacacgact
     2821 tccccttaat ccataaaata gacgattaat gtgggcttgc cacagatcga ccggttgaaa
     2881 atctcctacg agttgcggca atgcatcggg atccatactt ggtgaagcac tcatagttca
     2941 aacctatatt ccttagaaag aatgaatgaa aatgccagtg gcaacaaagt agatgaaaat
     3001 gcgtagaacc ggccggaatc ttaacaaaca cctcatggtg ctgcaaggca agctcgaaag
     3061 tccttgactc aagggcgcca tcgaaggtag agaacgtgga ttcttaaaaa gatccgggaa
     3121 agatttttgg aaatcacgat agtcgagtca cgccacctct acacagaaac ctgtatgaca
     3181 catcctttgc ccgtcatgcc gcgcagccac ctgttgggca tcttgatgtc gatcctgctt
     3241 tttacgatgc tcagcaccgc ttggggaggt gattctcccg ttcagaccga ccccccttcc
     3301 gtatttcaac cgaaagatac ccaacacggg ttttcccaat atgatactcc gcccgatcat
     3361 ttttctgatg ctccatccct ttatgataat tactcccaat cgcctctaat cccctcattg
     3421 gataaaaaac ccgggggagt atttctgggt ttagatttcg cagaaagttt cgaggtcaaa
     3481 ccgaccacca ggggcggcca tacacacata cccgtcaggc caactgaaac attccctccg
     3541 gacgtcacat cagtgtatct ggtgtttacc gtccatgaac atttatcgtc ctatcaaatc
     3601 attggccgtc tgttctctga attagaaacc ggaatgcatg ctcctcaatg gctcgacgaa
     3661 gatatcgtcg atctggctac ggaagacgag agcggatttc tgaaattttt cccaccggac
     3721 gggacctggc gaccagggca ataccgtgtc gaaatcttcg tgggatatat cgccaatgac
     3781 gtcaataagt tgggcaccat gcgatttacc attggtccac accctgccgc acccgcttta
     3841 ccctaagtac cctcttgtgg ccacagacct tttgtgccgg gctatttctt gactcaattc
     3901 aatgtttctt gtaaggtatt cagcagttta ccctaagcat atttttgggc cattccagcc
     3961 atcatttcgt gcaattttct tatacattcc agagatcgca tacacacaat tccttgatct
     4021 ccaaccatgc gccgggaggc aacgggtgtt aaaaaagagt ggaagctact tactcttatt
     4081 catttttatt ggtggactgt taggcagtat tttaggggaa attcttcaag tcgtggctcc
     4141 tcagggcacc gtacaaaata ttttcgttca ggcgttaaac ttaggcttag acccacccgt
     4201 gaccgtcaat ttggtcttga taaaattcac actcgggttc ctcctgaaaa tgaaccttct
     4261 caccgtttta ggcatgttcc tcggcgctta cgtttataaa cacatataga cgaactcaaa
     4321 cgcccgaccg tccctctcga aaccctctct tgccggcgtg gtcattcccg acagtccgta
     4381 ttgtgcatcc atggtgggct cgttgcggat agatcaccac ttgctaccgg cggggtaaca
     4441 gaattggaaa gggagggggt cgctatcccg gaattcgata agagaacatg aaagagggcg
     4501 ctggtcctga tttaaatacc gacctgaatc tgcttttggc ggagaacacg aatagtatcc
     4561 cgcaactgag cggctcgttc aaattccaag gccttggctg ccgccttcat atctttttcc
     4621 aatgcggcga tattctgttc taaatccttt tcgcccccat aggccgcaac cgcctctgcc
     4681 gctaaggaca catccacata atcagcttcc gccaattgat actccaaatc gtggatgcgt
     4741 tttttgatcg tctccggcgt gatcccatgc tcggcgttgt atgccaattg aatggttcgc
     4801 ctgcggccgg tttcgtccaa cgccctccgc atcgaatccg tgaccacatc gccatacaaa
     4861 atcacggtgc cttcccgatg acgcgccgca cgcccggctg tctgtaccaa ggcacgatat
     4921 ccccggagaa aaccttcctt atccgcatcc aacaccgcca ctaaactgac ttcaggcaaa
     4981 tccaatcctt cacgcaataa attaatgccg accagcacat caaacacacc cttacgcaga
     5041 tccctgatga tctccgcccg ctcaagcgtt ttaatatccg aatgcagata ccgcaccttt
     5101 aatccctgat cgtgataata ctcggtaaga tcttccgcca tgcgcttggt caacgtcgtc
     5161 accagcaccc gctgcccctt cgccactcgc agccggattt ccccgagcag ctgttcgacc
     5221 tggcccttcg ccggcttcac gtccatcacc ggatcaatca aacctgtggg tcgaatgatt
     5281 tgctcaacca gcgccttgcc ggcatgtttc agttcatacg gtcccggagt cgcggaaaca
     5341 tacaccacct gattcatgca cctttcaaat tcgggaaatt taaggggccg gttatccatc
     5401 gccgagggta agcgaaaccc gtactcgacc aaggtgtttt tgcgtgaacg gtctccctcg
     5461 tacatcccgc caaattgcgg aacggtggca tgcgattcgt ccacaatcag caaaaaatct
     5521 ttagggaaat aatccaacag ggtcggcggc ggctcgcccg gcgctcgtcc gctcaggtga
     5581 cgggaatagt tctcaatccc atggcaatac cccatcgttc gaatcatctc caggtcaaat
     5641 ctcgtccgtt gttcaatccg ttgcgcttcc agcaattggt tcgtccggcg aaagtaggca
     5701 atgcgttctt ccagctctga ttcaatgccg gtaatggcct gttcatagcg atcaggcgca
     5761 atcacatagt ggctcttggg atagatcgca attttcggca gcttccgcaa cgactggccg
     5821 gtcaacggat cgatttcata cagggcttcc acttcgtcgc cgaacaattc cacccggacc
     5881 gtattcgact cggacgaagc gggataaatc tcaatgacat ccccgcgcgc ccgaaacatc
     5941 ccacgctgca actcgagatc attccgcgaa tattgaatgt ccaccagctt ggccaaaatg
     6001 cgctcccggc gcatctccat gcccggctcc agatacaaca acatgtcgtg atagacctca
     6061 ggcgatccca ggccataaat acacgacacg gatgccacaa taataatgtc attgcgttgc
     6121 aggagcgccg tcgtggccgc atggcgcatc tggtcgatgg tgtcgttgat ggcggaatct
     6181 ttggcgatat aggtatccgt agtcggaata taggcttccg gctgatagta gtcgtaatag
     6241 ctcacgaaat attcgaccgc attgtcggga aaaaatgatt taaactcctg atacaactga
     6301 gccgccaacg tcttattgtg gaccacaacc agtgtaggtt tttgcacgtt gacgatcacg
     6361 ttggccatgg tgaacgtctt ccccgaaccc gtgaccccca ataacacttg atgcttaagc
     6421 ccttgttcta agccatcagt caacgcggca atcgcattcc cttgatcacc tttgggtgtg
     6481 taatcggact tgagagaaaa ggtattggtc gcttgcatca tgccaatgta tcacaaggcg
     6541 attgtgggag gcaggtcgat ggtgtccgtg gatcctcttg acgctcttat gctgatcagg
     6601 ccaggatcat ccgagaaccc ccgggtcacc cagcccatat aagaggagga cgtatggcca
     6661 caaccaccat cgcggaattt atgggacatg atcatgaccg tttggatgaa atattcaacc
     6721 ggtttcaggc attcaagaag caggattctc aaaaagcccg attactgttt caagaattgt
     6781 taaccggact gcaccgacat atcaggtggg aagaggagat tctttttccg ttctttgagg
     6841 ctcaagcgga aatggtcaga gaccatgggc cgaccggagt catgcgaaat gagcatcggc
     6901 agatcaagga ctttctggaa cagattcagg caaaagtcgc agcaggagat ctttccactg
     6961 aggaagcgga agaaggtttg cttcaggttt taactgctca caatgtgaag gaagagaaaa
     7021 tcctctatcc gtggattgac aattgcgtca gcgacgaaga gcgggcctcc ctcctcgccc
     7081 ggatgggaaa ctgattctcg ccgaccggca catcccgcgg ccagtcacgt tcacgtgctc
     7141 ctttctaaaa caatcccaca atatttcctt gtgcgtcgat gccgatcgat tgagccgccg
     7201 ggacacgcgg aaggccgggc atcgtcatca tatcccccgt aatgaccacc acaaatcctg
     7261 cacccatcgc gaggcgaact tctcgcacgg gaagcgtaaa tccggtcggg gctccctttt
     7321 tggcaggatc agtggaaaag ctgtactgcg tcttcgccat acaaatgggg aaatgaccaa
     7381 acccgttttt ttccagttca gcgaaacggg cgtgaacggc cggcggaatt tctacatcct
     7441 cagcccggta aatctctctg gcgatgattc gcactttttc tgccagcggc atcgtatcag
     7501 gatacagcac gcgaaagtcg gcgggctcct tttcgataac atcacgaacg gtttcggcca
     7561 aacacacggc accggcgccg ccttcagccc aatgattggc cagagccatt ttgaccccat
     7621 gatccgcgca cgcctgttcc accagttcca actcccgctt ggtatcactc gtaaattggt
     7681 taatggccac aaccgtggga actccgaatt ttttcacatt ttccaaatgg cgtaataaat
     7741 tcggcaaccc gtgacggagt gcgtcaagat cttcctgttc gaggtctttt tccttggccc
     7801 caccatgaag cttcaacgcc ttaatcgtcc cgacaaccac cgcacatgcc ggggtgagac
     7861 cggatcgtcg acatttaatg ttaaaaaact tctcggcccc gagatcggca ccaaaccctc
     7921 cttctgtcac cacatagtcc gcgagtgtga gagccgcttt ggtcgccaca acggaactac
     7981 acccatgggc aatgttcccg aacggaccgc catgaaccag ggccggatta tgttccaggg
     8041 tctgcacaag attggggttg agagcgtcct tgagtaacac ggtcatggct ccctccgcct
     8101 ggatatcttt tgcaaagacc atttccttgt tgaccgttct ccccactttg atatgaccta
     8161 aacgttgctg aagatcctgg aggtcggtcg ctagacacag aatggccatg acctccgagg
     8221 ctgcggtgat gtcaaaccgt ccttctctgg taaacccgtt ggatttcccc cccagtcccg
     8281 taaccacgga tcgcagtgac cgatcattca catccaacac ccgaccccac gtgacccgac
     8341 ggggatcaat gtggggtttg tgcccccaat acaaatgatt gtctaccagg gccgccaaca
     8401 tattgtgcgc tgacgtgatc gcatgaaagt cccccgtgaa atgcagattg atgtcggtca
     8461 tagggacgac ctgggcatac cctcccccac atccgccgcc cttcattccg aaacagggcc
     8521 ccaggcttgg ctctcgaacg gccccaacag cccgttttcc gatcttattc aagccatcca
     8581 ccagaccgat aagcgtcgtg gtcttgcctt ctccagccgt cgtcggactc atggcggtca
     8641 ccaaaataag cttgctctcc ggcttgtttg gaaatggcgg gagggacgct aagggaattt
     8701 tggccttata ccgcccatac agttcgattt gactcgcatc caattgaagt ttttccctgg
     8761 ccacttcggc gataggttgc aaggggattc ggttggcgat gtcgagatta atggaagaat
     8821 ggctcatcag gctgactcct caaaaacgtg catggggtta aggtctggat gttaaattca
     8881 ttatcgcata ctcgaagcgg acggcgggcc ctttctcccg ccccgtcgcc gaccggcctg
     8941 aggcaatacc ctcgtgttgg cattcaatca tgccagcccg tggtgatgga gcggggacgc
     9001 cgggacaaca aggaaaagca acaggtctat tcccgatgaa catgatggga tttgacctgg
     9061 aggaaacgag aaggaaggtc ggcctcaggt ctattccggc ataacaggca ccccggtccc
     9121 gcttggtatt cgaccccaag ttttgttaag tagaggacat gcataaactt ttctctacat
     9181 tcggctttgt attctggttc accatgtgtg gctggaccat caccttcgcc caggattttg
//
LOCUS       DATIAL010000418         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_76303, whole genome shotgun sequence.
ACCESSION   DATIAL010000418 DATIAL010000000
VERSION     DATIAL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76303"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VLA60_12905"
     CDS             complement(<1..431)
                     /locus_tag="VLA60_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HSF10306.1"
                     /translation="MKKSTSQSGGAFSAEEREAVYRAIFERRDVRKNFLPDYIPEETL
                     RRILLAAHHAGSVGYMQPWDFLIIQDPDTKHAVKNLFVQANDAAALLYRGKESGLYRG
                     LKLEGIQEAPINICVTCSRDRGGPSVLGRATVPETDLYSTC"
     gene            complement(428..1024)
                     /gene="cobO"
                     /locus_tag="VLA60_12910"
     CDS             complement(428..1024)
                     /gene="cobO"
                     /locus_tag="VLA60_12910"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HSF10307.1"
                     /translation="MSDHMHQARMERLKASVDRRIAEAQEEKGLLMVHTGAGKGKTTA
                     ALGMAIRCLGHGMNVAVVQFIKGAIDTAEERILKSFGSQVVFLRMGEGYTWETQDRER
                     DRLVAQKAWTEVGKFLQDPSFGMVILDELNIAIHHEYVSLEQVLKAVAQRPPTLHVVI
                     TGRGAKPELIEAADLVSEMKMIKHPFRKGIKAQKGVEF"
     gene            complement(1021..>2354)
                     /locus_tag="VLA60_12915"
     CDS             complement(1021..>2354)
                     /locus_tag="VLA60_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HSF10308.1"
                     /translation="ALLSAFRAQGRTVQPFKVGPDFLDPGHHQLASGRTSRNLDGWML
                     GAVLNRTIFQKAAHGADLSIIEGMMGLFDGSSPVKEAGSTAELAHQLNAPILLVVDGS
                     AMARSAAAMVYGYAQFDSSLNVAGVIFNRLISEGHYLLLKEAVEKETRIPVVGYLGTD
                     SDVTIPDRHLGLRTAMEGSGTELYTKLGQLASATIDLGRIEQLAQASPEMLVTNSGMP
                     AKDSSLVNRSVRVGVAFDPAFCFYYPENLQFLEQAAGELVRFSPMNDPSLPDVDLVYL
                     GGGYPEIYAEALQRNSAMRHSIQAFAGRGGVVYAECGGLMYLANTLRDFNGTVYDMVG
                     VIPAETAMSRTKMTLGYRELTVTHTGLLGEQGAKIRGHEFHYSTLHPKGDLEYLGHIT
                     DAQGRDRGGDGITVGNVIALYTHLHFSSHPHVPAALVEAARGEAAMRGNRL"
BASE COUNT          570 a          701 c          537 g          546 t
ORIGIN      
        1 cagcacgtac tatacaaatc cgtctcggga acggtcgcac gaccgaggac tgacgggcca
       61 ccacgatcac gagagcaggt cacacagata ttgatagggg cttcttgaat tccttcaagc
      121 ttcagacctc gatacaaacc cgattccttt ccccgataca gcaaggctgc cgcatcattg
      181 gcttgcacaa aaaggttttt cacagcatgc ttggtgtccg gatcctgaat gatcaaaaaa
      241 tcccatggct gcatataacc gacagagccc gcatgatgag cagccaacag aatgcggcga
      301 agcgtttctt caggaatgta atcgggaagg aaatttttcc ggacatctcg tcgttcaaat
      361 atcgcacgat agacggcctc ccgctcttca gcggaaaagg ctcccccgct ttgagaggta
      421 ctctttttca aaattcgact cctttttgag ccttgatgcc tttgcgaaag ggatgcttaa
      481 tcatcttcat ttccgaaacc aggtcggcgg cctcgatgag ctccggcttc gcaccccgcc
      541 cggtaatgac cacatgaagt gtgggaggac gctgagctac agccttcagc acttgttcaa
      601 gcgagacata ttcatgatgg attgcaatat tcagttcgtc cagaatgacc atgccaaagg
      661 atggatcttg aagaaacttt cctacttctg tccacgcctt ttgagccacc agtctgtccc
      721 gttcacgatc ctgggtttcc caggtatacc cttcacccat tcgaagaaat accacctggc
      781 tgccgaagct cttcaggatt cgttcttctg ccgtatcgat ggccccttta ataaattgca
      841 ccaccgccac gttcatgcca tgtccgagac atcggatggc catgcccaat gccgcagtgg
      901 ttttgccttt gcctgcaccg gtgtggacca tcaatagacc cttttcttcc tgtgcctcgg
      961 caattcgtcg atccacagaa gctttcaacc gttccatgcg agcctgatgc atgtgatcgc
     1021 tcatagccta ttccctctca tcgcagcttc accccttgcc gcttcgacca aagccgcagg
     1081 gacatgaggg tgactggaaa aatgcaaatg tgtatacagg gcaatgacat tccctaccgt
     1141 aataccatct cctccccggt cccgtccctg cgcatccgtg atatgaccga gatattccag
     1201 atctcccttg ggatgaagcg tcgaatagtg aaactcatgt ccacggattt tcgccccctg
     1261 ttctccaaga agtcccgtgt gagtcacggt caactctcga taccccaagg tcatctttgt
     1321 ccggctcatt gcggtttcgg ccgggatcac cccgaccatg tcgtacaccg ttccattaaa
     1381 atctctgaga gtgttggcca gatacatcaa accgccacat tccgcataga ccactccacc
     1441 ccgtcccgca aaggcttgaa tggagtgacg catggcgctg tttcgttgta acgcctccgc
     1501 gtaaatctcc ggataccctc ctcccagata caccagatcc acatcaggga gtgacggatc
     1561 attcatgggg gaaaaccgga ccaactcccc agccgcctgt tcaagaaatt ggagattctc
     1621 cggatagtaa aaacaaaaag ccgggtcaaa agccactccg actcttacag atcgattcac
     1681 cagactggaa tccttagccg gcatgccaga attcgtgacc agcatttccg gactggcttg
     1741 agctaactgt tcgatgcgac ccaaatcgat ggttgcggaa gccagttgac cgagtttggt
     1801 atacaattca gttcccgaac cttccatagc cgtccgtaat cccaaatgcc gatccggaat
     1861 ggtcacgtcc gaatcagtgc cgagatagcc cacaaccgga atacgggttt ccttttctac
     1921 cgcctccttt aataacaggt aatggccttc actgataagt cggttaaaaa tcacaccggc
     1981 gacattcaac gaagagtcga attgtgcata gccatagacc atcgcggctg cggagcgggc
     2041 catggcactc ccatccacga ccaggaggat cggcgcgttc agttggtgag ccaattcagc
     2101 cgtgctcccc gcttctttga ccggagaact tccatcaaag agacccatca tgccttcaat
     2161 tatcgagagg tcggctccgt gagctgcctt ttgaaaaatc gttcggttca atactgcccc
     2221 gagcatccac ccatcgaggt tccgggatgt ccggccactg gccaactgat gatgccctgg
     2281 atccagaaaa tccggcccga ctttaaacgg ttgaaccgtg cggccctgag cccgaaacgc
     2341 cgacaacaac gcca
//
LOCUS       DATIAL010000419         3444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1475020, whole genome shotgun sequence.
ACCESSION   DATIAL010000419 DATIAL010000000
VERSION     DATIAL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475020"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VLA60_12920"
     CDS             <1..408
                     /locus_tag="VLA60_12920"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF10309.1"
                     /translation="QNIPLGFLEMILLSLKNAGLIGSRRGAHGGVYLIKSPREVTLGQ
                     IIRLLDGPLAPIACVSQTAYQKCRDCPHAGTTACPIQRIMLDVRNAIASVLDHYTLQD
                     FVFPRQGGAVPNMKKQPMSSATQKKKKSSDNQS"
     gene            460..681
                     /locus_tag="VLA60_12925"
     CDS             460..681
                     /locus_tag="VLA60_12925"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HSF10310.1"
                     /translation="METHWRSVVKGVSWRAVGTLDTTLLVFIFSGELLLAASVGLTEL
                     VTKIFLYWVHERVWQKIRWGRIYPTTSSP"
     gene            715..1125
                     /locus_tag="VLA60_12930"
     CDS             715..1125
                     /locus_tag="VLA60_12930"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HSF10311.1"
                     /translation="MARTILISGALVALLGVAGGAFGAHGLRPLLSEHMMNVFETGVR
                     YQVFHALAMLVAGLSLAYAPIRYFRWAGWAFSLGIVLFSGSLYVLSISGVRALGMITP
                     FGGLCFIVGWGLLAWGYWKAFPSESTPISPPNGI"
     gene            complement(1150..2082)
                     /locus_tag="VLA60_12935"
     CDS             complement(1150..2082)
                     /locus_tag="VLA60_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF10312.1"
                     /translation="MMEPFSFEQLSPFLAHKSMAVIEVSLRLLLIGIVAYVGVRALSF
                     ALHRLETLLLTMRKDQDKNQLDAEKRVKTLTGLLRTICLTLLWVVAVVMSLDQVGLDI
                     TPILASAGIVGLAVGFGAQNLVRDVINGFFMILENQVRVGDVAVVNGTGGLVEGISFR
                     TITLRDLNGTVHIFPNGTITTLSNMTKEWSYYVMDIGVAYKEDTDRVTAVIKDVGKEL
                     QEDPEFGPNMLEPIEVLGVDQFADSAVVIKARLKTLPIKQWVVGREYRRRLKKAFDAK
                     GIEIPFPHRTLYMGEASLPFTVNGTLAQVEREAP"
     gene            2678..>3444
                     /locus_tag="VLA60_12940"
     CDS             2678..>3444
                     /locus_tag="VLA60_12940"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSF10313.1"
                     /translation="MSLGLALLVVAGGASVSEAAVLTKEDFKLYGYVEASYTQNFNFP
                     KNSGANANDLRIFDGDANSFRPNMAQLVFEKEGTNASNVVERAGFRLKLDVGEDAQFT
                     GGRTGDDIDFQEMYVQYVAPLGNGLDIRIGRMNALVGYEVIESPYNNNFSRSWLFGLG
                     QPFTITGIRLAYAVNEQVSVLMSVLNSYTGLTEDNNTAKSVEGLVSYRPTEALGLNVY
                     GIVGKESSNTRSGSGTRVLVGGYATYQATDQLGFVVEG"
BASE COUNT          823 a          793 c          939 g          889 t
ORIGIN      
        1 cagaatattc cattgggctt tctggaaatg attttgttgt cgttaaagaa cgcgggactg
       61 attgggagtc gtcggggcgc ccatggcggg gtgtatttaa ttaagtcgcc ccgagaagtc
      121 accttggggc agattattcg ccttctggat ggcccactcg caccgattgc ctgtgtgagt
      181 cagacggcgt atcagaaatg ccgggattgt cctcatgcgg gaacgacggc gtgtccgatt
      241 cagcgcatca tgctggatgt gcggaacgcc attgcatcgg tccttgatca ttacactctt
      301 caagattttg tattcccccg gcagggaggg gcagtaccca atatgaaaaa acaaccgatg
      361 tcatctgcga cacagaagaa aaaaaaatcg tcggataatc aatcttagtc agtctttttt
      421 gctgtcctca cgcggtaagg tcaacgataa gaggaaccta tggaaactca ttggcgtagt
      481 gtggtgaaag gtgtgagttg gcgggcagtc ggtacattgg ataccactct cctggtgttt
      541 attttttctg gtgaactcct tcttgccgct tccgttggcc tgacggaatt ggtcacgaaa
      601 atatttttat attgggtcca tgaacgagtc tggcaaaaga ttcgatgggg gcgaatatat
      661 ccgacaacca gttctccgta aattttactc accgcgatcg attggattga agatatggca
      721 cggacaatac tcatcagtgg tgcgttggtg gccctccttg gtgtggcagg tggtgcattc
      781 ggggcgcatg gcctgaggcc cctgctttca gagcacatga tgaatgtctt tgaaacgggt
      841 gtgcgttacc aggtgtttca tgccttggcc atgcttgttg caggattaag cctggcctat
      901 gcccccattc gctatttccg atgggccggc tgggcctttt cccttggtat cgtgttgttt
      961 tcaggaagtc tgtatgtctt atctatttca ggtgtgcggg cactggggat gatcacgcct
     1021 tttggaggcc tgtgctttat tgtgggatgg ggcctgctgg cgtggggata ttggaaagcc
     1081 ttcccatctg agagcactcc aatatcccca ccaaatggca tttaaggaag gcacaactcc
     1141 ttaacagggt tagggcgctt ccctctccac ctgggcgaga gtgccgttga cggtgaaggg
     1201 gaggctggcc tctcccatgt agagggttcg atgtggaaag ggaatttcaa tgcccttcgc
     1261 atcaaaagct tttttaagcc ggcgacggta ttctctcccg actacccatt gtttgatagg
     1321 aagagttttc aatctggctt taatgaccac cgccgaatcg gcaaattgat ccactccaag
     1381 aacttcaatg ggttcaagca tgttggggcc gaactccggg tcctcctgta attccttgcc
     1441 tacgtcttta atgacggcag tcactcgatc agtatcttct ttatacgcca caccgatatc
     1501 cataacataa taggaccact cctttgtcat gttggagagg gtcgtgatcg tcccattggg
     1561 aaaaatatgc accgtaccgt ttaagtcacg aagggtaatg gttctgaagc tgatgccttc
     1621 cactaaaccg ccggtgccat tcaccactgc cacatcacct acccgcacct ggttttccag
     1681 gatcatgaaa aagccgttaa tgacatccct gaccaggttt tgcgcgccaa agccgaccgc
     1741 aaggcctacg atgccggcac tggccagaat cggggtaata tccagaccta cctggtccag
     1801 gctcatcacg accgcgacga cccatagtaa cgtgaggcaa atggttcgaa gtaaaccagt
     1861 caacgtcttg acccgctttt cggcgtccag ttgatttttg tcctgatctt ttcgcatggt
     1921 gaggagcaag gtttcgagtc tgtgcagggc gaaactaaga gctcgtaccc cgacataggc
     1981 gacaatccct attaggagaa ggcgcaggct gacttcaata acggccatcg acttgtgtgc
     2041 cagaaaggga gagagttgtt caaatgaaaa cggttccatc attcacgacc tccttgagag
     2101 aatgttcata ggttgaacgc attgggtaaa cacatgccga ttgaaaaacg caggaaaagt
     2161 atcctttaaa gggttcagaa aatccgcaag cacggagagg atagagatac cacatcacgc
     2221 caatatccgc ccaggaaaat acgaatggcc tcgacaaccg gctttctgtg cctggccagc
     2281 tctttctaaa ggtcccgcgg gtttttcttt tgaatctccc ttgacactaa ggaaaacagg
     2341 tggtaaaaaa ctcaaaatag gtcgccaagg acaacgacgt cccatgggtg acctcccata
     2401 caatcgaacg cggacaatga agtcctcagc caaagaaatt ggttggggat ttttttttgc
     2461 cttttgaccc catgcccgtt cgttggactt agggaaaccc aatagagtct cgtcaatgaa
     2521 gcgtggctga tcattagcgg gggaaggtgg cgtaggaaat tcctggtcgt gggatggcga
     2581 gtgcgacctc aaaaacacgg agggctacat gattggagac ctaatagctg gaatcacctt
     2641 tcaccactca cagaaggctc ctaggagtgg agggttgttg agccttggtc ttgcgctgct
     2701 tgtggtggct ggcggagcga gcgtgagtga ggcggcggtg ttaaccaagg aggatttcaa
     2761 actctatggg tatgtggagg catcctatac gcaaaatttc aactttccaa aaaattccgg
     2821 ggccaatgcc aatgatttac ggatctttga cggggacgcc aattctttca gacccaatat
     2881 ggcgcaattg gtctttgaaa aggaagggac caatgccagc aatgtcgtcg agcgtgccgg
     2941 cttccgactg aaattagatg ttggggagga tgcgcaattt acgggtgggc gaaccgggga
     3001 tgatatcgat tttcaggaaa tgtatgtcca gtatgtcgcc ccgcttggca atggtctgga
     3061 tattcggatt ggacgaatga atgccttggt gggctatgaa gtcattgaga gtccctacaa
     3121 taacaatttt tcccgatcct ggttatttgg tttgggtcag ccctttacga tcaccggcat
     3181 tcgtctggcg tatgccgtca acgaacaggt ttcagtgtta atgagtgttc tcaattctta
     3241 tacgggtttg acggaggata ataatacggc gaaatcggtg gaggggttag tgtcttatcg
     3301 ccccactgaa gcgcttggat tgaatgtgta tggcattgta ggaaaagaga gttcgaatac
     3361 gagatccggg tcgggcacac gggttcttgt cggcgggtat gcgacctacc aggccactga
     3421 tcaattgggt tttgtggtgg aagg
//
LOCUS       DATIAL010000420        27797 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_505897, whole genome shotgun sequence.
ACCESSION   DATIAL010000420 DATIAL010000000
VERSION     DATIAL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 27797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27797
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505897"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(562..1494)
                     /locus_tag="VLA60_12945"
     CDS             complement(562..1494)
                     /locus_tag="VLA60_12945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HSF10314.1"
                     /translation="MSDTMVSLRRKIGGAGDLQSVVRTMKALAASSIGQYEQSVSALA
                     DYARTVELGLSVCFREDSPMALMGEQKRQSDAEAIGAIVFGSDQGLVGQFNEVVADYA
                     IKTLAARSGHHSVWAVGERVQSRLADAGLSLEGIFPVPNSVKAITPLVGKILLESEAA
                     HSRGKIHELHLFYNRPTSRAVYAPVNQRLLPLDETWRSELTTIPWPTGNLPEVIGSST
                     ATLRALIHEYFFISLFRACAESLASENASRLTAMQRAEKNIDELLEDLNRHFHQLRQS
                     GIDEELFDVISGFEALAKKPAARECHAGDPHIES"
     gene            complement(1491..1862)
                     /locus_tag="VLA60_12950"
     CDS             complement(1491..1862)
                     /locus_tag="VLA60_12950"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSF10315.1"
                     /translation="MQVQINTDRHIKGHEALAAQVSGVVKSALSRFSDSITRVEVHLS
                     DENSDKKGGKDDMRCVMEARLEGRQPVAVTNQAATVDQAVDGAAEKLTRMLESTLGQL
                     RDQKKHRTDPPPPGTEFPEES"
     gene            complement(1891..3054)
                     /locus_tag="VLA60_12955"
     CDS             complement(1891..3054)
                     /locus_tag="VLA60_12955"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10316.1"
                     /translation="MTQTTTLLTRELIAELVAAHRPPCLSLYQPTHRRHPENQQDPIR
                     FRNLVKELETSLRQKYPAVETRLLLEPFEALTNDHDFWTHTHDGLAVLAAQGLFHVFR
                     LQWPVAELVVVADSFHTKPLRRFLQSVDRYQVLGLSLRTIRLFGGNRHALDEIDLVPG
                     FPRTIEEALGDELTEPHSTVASYGGVGQASGAMHHGHGGKKDEVDIDADRFFRAVDRA
                     VLERYSRPSGLPLILAALPEHHHRFRKVSHNPFLMAEGLTINPDALQMDELRERAWQV
                     VDPQYQSQMATVTDEFAVAKSKGLGSDDLAKVAQAAATGRVATLLIEADRQIAGRVDS
                     ATGQVEFADLSHPQVDDLLDDLGELVEKMGGRVLVIPAEKMPGRTGLAATYRH"
     gene            complement(3430..5973)
                     /locus_tag="VLA60_12960"
     CDS             complement(3430..5973)
                     /locus_tag="VLA60_12960"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HSF10317.1"
                     /translation="MSRLTKSVPGLTSAEVARRLAAEGPNLLPGGTPKSMFAIVLGVL
                     TEPMFLMLLVAGGIYLALGDRAEALFLLGFVIVVIGITLAQERKTQRALESLRDLSAP
                     RALVIRDGQEMRIPGAAVARGDILVLREGDRIPADALLLEGQLAVNESLLTGEAVPVA
                     KSPGAANAAFKQPGGDGTPFLFASTVVTKGVGLAEVRAIGATTAVGRIGQALAATKEE
                     PSGLQQSSRKLIRNLTVAGLLLASAQILLGWLWNGRTFLESLLSGIALAMAILPEEIP
                     VILTVFLALGAWRISKQKVLTRRVSAVEALGAITVLAVDKTGTLTQNRMEVAELATAD
                     EVFRNTDANSLPEAFHELTEFAMLATPSDPFDPMEKAIQNFGHRWLIGTEHVHDGRTP
                     AFQYDLSPDILAMTQVFSSTEPSHHLLATKGAPEAVADLCHLPQAERDEIQRQVTTMA
                     ERGLRVLGVAKGDWQVSEWPQNQHDFTFIFLGLVGFNDPPRPEVPAAIAECRKAGVRL
                     IMMTGDHPATARAIASQVGLSERPEIMTGSDIAALSDAALCEKLQHVDLCARLQPEQK
                     LRLVQVLKTDGEVVAMTGDGVNDAPALKAADVGIAMGERGTDVARESAALVLLDDSFA
                     SIVAAIRQGRRIYDNIGKATRFTFAVHMPIIMLVLVPAFLQWPVLLMPVHIVLLELLI
                     DPACSIVFEAEPESPDLMTRPPRQVSASPFATANLTYAVIQGAGMAALLLLGHGVLVG
                     QGVSETESRLAVFTGLVLGVFLLVLSNRDLSRPAIMGLAANNPWIKRMFGAVALLLIV
                     VMTVPFLRGVMGFAPAGMHQLIASLLLLVGVSLWLETLRRLGGRLLFEK"
     gene            complement(5970..7523)
                     /locus_tag="VLA60_12965"
     CDS             complement(5970..7523)
                     /locus_tag="VLA60_12965"
                     /inference="COORDINATES: protein motif:HMM:NF009884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternate F1F0 ATPase, F1 subunit alpha"
                     /protein_id="HSF10318.1"
                     /translation="MNNPIENLHSLFDKTFAGMSHAREDFKPGMEAHEIGRITNVSTG
                     IARVSGLPRVGFEEVLKFPGDVYGIAFNVDEDEIGVVLLGDYWQLHAGDEVERRGHVM
                     DVPVCDKLIGRVINPLGRPLDGHGPVVSAERLPIERPAPPIMNRAPVTVPLQTGIKVI
                     DALIPIGRGQRELILGDRQTGKTAIALDTILNQRDQNVLCVYCAIGQRASGVAKVVAT
                     LREKDAMEYTIVVVTEGNDPPGLAYIAPYAATSIAEHFMEQGRDVLVVYDDLTHHARA
                     YRELSLLLRRPPGREAFPGDIFYIHSRLLERATHLRKDLGGGSLTALPIIETEAQNIS
                     AYIPTNLISITDGQIYLSPSLFELGVLPAIDVGRSVSRVGGKAQRTVYRAVAGNLKLA
                     YAQFEELETFARFGARLDDDTRAIIEHGRRIRACLKQPEFAPVSVPAQITLLLALIEK
                     LFDLVPLDQMMKAEQALHKAALDIPLEVCKRLEAATKLNDEDRQTIIDIARNALTPFQ
                     HNAPHKETS"
     gene            complement(7507..8325)
                     /locus_tag="VLA60_12970"
     CDS             complement(7507..8325)
                     /locus_tag="VLA60_12970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10319.1"
                     /translation="MLIDWFTVVAQALNFLILVWLLKHFLYKPILNAIDAREKRIAAE
                     LADADAKKAEARQERDAFKRKNEEFDQERAALLTNAVNQATAERQQLIDNARKESDTL
                     RAQWQEALRSEHQSLSEELTRRTREEVFAIARKTLADLTTTTLEEQMADAFIRRLHKL
                     SSEEKGALRSAFEAASTPAIIRSTFDLPSAQRTAIEEVVKEVLAIETQVQFETAPDLV
                     SGIELTANGHKIAWSIGHYLASLQKRVDDLLNMRPKAATKAIAHPERHANEQPN"
     gene            complement(8330..8611)
                     /locus_tag="VLA60_12975"
     CDS             complement(8330..8611)
                     /locus_tag="VLA60_12975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HSF10320.1"
                     /translation="MDSMTLIAMASIITAGLTTGIGCIGPALGEGRAVSTALTSLAQQ
                     PDAANTITRTLFVGLAMIESTAIYCFVVSMILIFANPFWNHVLAQAAGK"
     gene            complement(8643..9344)
                     /locus_tag="VLA60_12980"
     CDS             complement(8643..9344)
                     /locus_tag="VLA60_12980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HSF10321.1"
                     /translation="MHLSPDDIIFWHYGFIKLNATIVFTWGVMFVLTVGSLVITCHLS
                     TGLTRSRWQNLLEIVVTAIGKQIEEVGLSQPRKYLGFLGTIFLFVATASLCTIIPGYE
                     PPTGSLSTTVALALCVFVAVPLFGIEEQGVGGYLQSYLKPTVIMLPFNIISELSRTLA
                     LAVRLFGNMMSGAMIVGILLTITPFIFPIVMSLLGLLTGMVQAYIFSILATVYIAAAT
                     RTRQMKLEPDSGAQD"
     gene            complement(9334..9645)
                     /locus_tag="VLA60_12985"
     CDS             complement(9334..9645)
                     /locus_tag="VLA60_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HSF10322.1"
                     /translation="MSEFFMMVPALVTGIVLGAMFFGGLWWTVRKGLSSTQPAIWFIG
                     SLLLRTCLTLAGFYVVSDGQWERLLVCLVGFTIARPIVTRLTGLAENPSRPAQEPSHA
                     P"
     gene            complement(9638..9976)
                     /locus_tag="VLA60_12990"
     CDS             complement(9638..9976)
                     /locus_tag="VLA60_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HSF10323.1"
                     /translation="MTEDPEEKPLREETTLARQVGTKAARKLKAQRHTTRTIWFGLGM
                     MGLIGWSVAIPTLLGAALGLWLDKQYPGPHSWTLTLLFIGLIIGCMNAWHWVTKEDRE
                     IRAEQENHHE"
     gene            complement(9969..10373)
                     /locus_tag="VLA60_12995"
     CDS             complement(9969..10373)
                     /locus_tag="VLA60_12995"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HSF10324.1"
                     /translation="MQPTLMTLKILLPFRIFAEQHAVTRIVAGTREGAFGILPQRLDC
                     VAALEPGILIYETNGDDEQYVALDEGVLVKNGREVLVSVRNAIAGTDLHQLREAVERE
                     FLTLDEHEQSLRSVMAKLESSFVRRFADFRHD"
     gene            complement(10412..11890)
                     /gene="atpD"
                     /locus_tag="VLA60_13000"
     CDS             complement(10412..11890)
                     /gene="atpD"
                     /locus_tag="VLA60_13000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HSF10325.1"
                     /translation="MDTNINHLNLGAVVSVRGSVVDIRFEDHLPPIYSLLRTGQEGRI
                     AIEVLAQLDTHRVRGIALTPTQGLARGMVVEDTGGPLMAPVGKGILSRMFDVFGNSID
                     REPPPTEIQYRTVHRAPPSLARRSTKSEIFETGIKIIDVLLPLERGGKAGLFGGAGVG
                     KTVLLTEMIHNMIGHHQGVSIFCGIGERCREGEELYREMKEAGVLPNMVMVFGQMNEP
                     PGSRFRVGHAALTMAEYFRDDEHRDVLLLIDNIFRFIQAGSEVSGLMGQMPSRLGYQP
                     TMGTELSRLEERIANTDSGAITSIQAVYVPADDFTDPAAVHTFSHLSASIVLSRKRAS
                     EGLYPAIDPLQSNSKMATPGTIDERHYRIAQDVRRTLAQYAELKDIIAMLGLEQLSLA
                     DRKVVARARRLERFLTQPFFTTEQFSGLKGQLVSRKDALDGCERILGDEFKDYPEHTL
                     YMIGRIDEAKEKAKSGKPAVQDEQESNNEPSDDPKLNGKSER"
     gene            complement(12435..13001)
                     /locus_tag="VLA60_13005"
     CDS             complement(12435..13001)
                     /locus_tag="VLA60_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF10326.1"
                     /translation="MGDGLFQKILIPVDFAPCSREAFLVGLRLARNFQSEVILLHVID
                     TKSLDALNALGLALPSEEKAQKKRLHHQARILARGLLALPEAKGIKISRLLSEGKPFM
                     EIARMTRTEQVDLVVMGSYGGQTGDITRIFFGSTAEKVVRTVTCPVLCVPHPYHKRQP
                     FTDVDAVSKPLKTEPNKKRTKPSSGSRK"
     gene            13408..14316
                     /locus_tag="VLA60_13010"
     CDS             13408..14316
                     /locus_tag="VLA60_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF10327.1"
                     /translation="MKILCAVDGSEFSLWALECVGKLFRHSMKELVLLHVVDPRILRG
                     GGVRRSQKDEPTTKDISKKLEAAGRKVLTDCAHRMEVVLSQAATKPFAAIKTALVKGH
                     VADSIITYAERSRPDLVVVGSRGMNDLPGYLLGSISRTILTHGPCSVLTVKSPLDVPA
                     SVLLALDGSKPSKFAANRVKEWLSPEEVTLHLVSVVPDILTDASKKLFPKSRLKTLVA
                     PLQQHAQECLEQYRELFLKEGFQVVGKRLQGSPREKIVESVPVCHAQLVVMGSKGLTG
                     VERFTMGSVSEWVSAYAPSSVLVVRH"
     gene            14406..15722
                     /locus_tag="VLA60_13015"
     CDS             14406..15722
                     /locus_tag="VLA60_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF10328.1"
                     /translation="MKTGKTERSHVTSAKTTGAWVGKLLGFVLAGSMVSTGAFGKHLD
                     LSAPHADISLDCHGKFLGKQVPVETLKEMVAGHARWLADHQDPKGTQANFCGADLSGV
                     EFRETDLSGANFQGANLTRAVFTAAKLVHVNFHKANLTETSLSSVDLQGANASGGTFR
                     LADFRKANMTQANFAGADLREANLAESRFRLAILRGADLEDAYLVRADFEMANLAEAS
                     LIRVDGREANFQRALLANAKLIDADLNRSNLQEADLRSADLSGADLGLANLNEAMLPH
                     ARLSEANLDRAVLFKANLEGANCAGASFQNADALEANLVNSNLLRATLDEAYLAKANL
                     QGANLEGADVSWANLVEADLSQSNLEFAVLFRTNLQEAKLDGARFYGADLRGANLEKA
                     SLQGTHLEGVAIRYAHGLNQAQLNQSCVDPRTRLPEGLVGPSPCLR"
     gene            15835..16479
                     /locus_tag="VLA60_13020"
     CDS             15835..16479
                     /locus_tag="VLA60_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10329.1"
                     /translation="MFTLFEPGGMRIPYVSGVFRQRNVEKISNASPIGQVKVGQENPP
                     SISQKNAGPSSLQARLYEQVNSFTQSSRAKIFARDIMSSPVVTLSVTSSLAQAWDVMN
                     SRRFRHIPILGADDVVVGMLSDRDLFRGTMESVLSGTSESHTQAGGSIQSLVSRPVLV
                     ASPEAELRALARVLLEEHIGALPIVSEAVGLVGMITRSDILRTLVAHPDFDQWV"
     gene            16560..16913
                     /locus_tag="VLA60_13025"
     CDS             16560..16913
                     /locus_tag="VLA60_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10330.1"
                     /translation="MLNQGEKLLIVHRRLFEKDTPRFFVGEVLIYEAGIVKVKGYTFV
                     KDLFSGNMQKKSDLRTKVMSIVSGTLIVYQLPVTALLDSMRFSLDQDGGLVLTDEGGF
                     SMDVSESLYKHETHQ"
     gene            complement(17284..18543)
                     /gene="pfp"
                     /locus_tag="VLA60_13030"
     CDS             complement(17284..18543)
                     /gene="pfp"
                     /locus_tag="VLA60_13030"
                     /inference="COORDINATES: protein motif:HMM:NF041103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HSF10331.1"
                     /translation="MDSPSPKKFAILVGGGPAPGINSVIEAATIRSRLCGVEVLGIED
                     GFQWLMKSDLSHTQPLTRETVSHLHFRGGSLLGTSRANPTKRPQDLSTTLTSLQKLDV
                     TGLITIGGDDTAFSALKLAELASGRLHVVHVPKTIDNDLCLPHGMPTFGFQTARHMGV
                     ELVKNLMIDAHTTSRWYFVVTMGRKAGHLALGIGKAAGATLTLIPEEFKGTTLKLGHL
                     TNILVGAMTKRLVQGRSDGVAVLAEGLSEFLDQDDLALLGGVERDEHGNIRLAELDIG
                     KVLKETVSQKLKHSGIKITIVSKNIGYELRCADPIPFDMEYTRDLGYCAAQFLLDGGN
                     GAMVSIVNGRFTPLPFSDIVDSATGRTRVRMVDVDSESYKIARAYMARLEPEDLANME
                     TVTQYAEMLNMTPDQFRSTFEAVANPF"
     gene            complement(18605..19522)
                     /locus_tag="VLA60_13035"
     CDS             complement(18605..19522)
                     /locus_tag="VLA60_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF10332.1"
                     /translation="MKTQNAPVKYKTILIPVDDSSQAEEAVQGVQALSPPERVLLLHT
                     ISIPQLAYPGTGMSVGHDFSGAAERALREEGARILEKAASLLPPECGKVSQHLEIGIP
                     ASVILSMTEHESVDLIIMGSRGLGTIKEHLIGSVSHRIATHASCHVLLVKAPVVPFKN
                     ILLLVEYPLDAGWAIEFLSQKPFRGTPHVTVLHVIPFAQPVFPMGALLPDSWKKELQD
                     VGARLTKDVADKLSSLGYSVESIVEPGAPSSVIQRQISRLQPQLVLMGTPGRSPIQRL
                     AHGSVSHATIHHSPCSVLLIRDPISHAPA"
     gene            complement(19901..20182)
                     /locus_tag="VLA60_13040"
     CDS             complement(19901..20182)
                     /locus_tag="VLA60_13040"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HSF10333.1"
                     /translation="MKKYRNIGHHERVIRVGVGFLLLALSGFSFLPGWGDLILMIVGL
                     IALLTGIIGYCPAWQALGINTCPPQKPDHPLSRSNPAIHEQADPSSHRS"
     gene            complement(20225..20584)
                     /locus_tag="VLA60_13045"
     CDS             complement(20225..20584)
                     /locus_tag="VLA60_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10334.1"
                     /translation="MLLIATIDPILRKNLQQTLTQKGDPFLLLDEGENIIESVLCHNP
                     SLVVVDLYLTQPSGLEILRQLREKGFSGKVVVLGGQSNQSLAPEASQLGAIQIIGRPF
                     NANQVLGAVRVASGDLD"
     gene            20932..21474
                     /locus_tag="VLA60_13050"
     CDS             20932..21474
                     /locus_tag="VLA60_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HSF10335.1"
                     /translation="MRALLVFVCFLGCSGTGWAKDIELVALGVRGGMNFKDAGLQPGE
                     KEDFEQFDVFGIMGLPWRWEGTSGWEVRSQVWGTAGALRGAGDLGFITTLTPGLALKI
                     PSWWLIIDGGAGGALISKWKFGRQDIGGPFQFIAHVGVGIQLPGHMVVGWRFHHMSDA
                     TIYGKSRGVDLHMLELSYAF"
     gene            complement(21713..22132)
                     /locus_tag="VLA60_13055"
     CDS             complement(21713..22132)
                     /locus_tag="VLA60_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10336.1"
                     /translation="MATPNLHIKARDLMQTRMVAVTRQYSARDLSILIHSGTFSGVPV
                     IEPGNHLVGMVTEFDVLKALADGKDLHGLKAEDVMTMNPVTVEETATAEEIVKHMIKH
                     QIIRIPVVRDGKLLGMISRTDLLNHLIDNHLINVYGG"
     gene            22644..23561
                     /locus_tag="VLA60_13060"
     CDS             22644..23561
                     /locus_tag="VLA60_13060"
                     /inference="COORDINATES: protein motif:HMM:NF039293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10337.1"
                     /translation="MSVIIQRFEPGTLQAKVQVRTAHAMQRGAIVTIPTRFELVEQAG
                     VNFLIRIVSNLVRKVQANVDQRHPSKAVNTDQDPFLPYDQDLFVADISDTHVCLLNKF
                     NVLDHHILMVTRTFQEQESFLTLSDIEAVLLCLTEFEGLAFYNAGEAAGASQRHKHLQ
                     MVPLPLTAEVSHLPIEPLLETARFEGTIGRAPGLPFSHVLVRMNPEWIAFPLKGAQEL
                     LKQYLHLLQTLGLADGGTGEETRSPGPYNLLVTRQWLLLVPRSTECFEDISVNALGFA
                     GGFLVKNEAQLDRLKTCGPMTALRHVALC"
     gene            23676..24242
                     /locus_tag="VLA60_13065"
     CDS             23676..24242
                     /locus_tag="VLA60_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HSF10338.1"
                     /translation="MDLQIDSRNVTMTPRWKMEIESRMADLQAGHDDLIHGRVTLTKN
                     AHHKKSQNVAEALVVVTMPGRHTLTARKEEKTFEEAIRAAFFAMAIEVKKFRDKRASK
                     EIRVPPIPLRGVISKLFPKEGYGFLLQDGGGEVYFHKNAVHGMKFEELEDGVEVSFNV
                     EDGEKGPQATTVNPLPSVPGAVDKAAFS"
     gene            complement(25779..26912)
                     /locus_tag="VLA60_13070"
     CDS             complement(25779..26912)
                     /locus_tag="VLA60_13070"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSF10339.1"
                     /translation="MLKRTVTLFQLLGFKVGIDWTWPFLALLIVWSLAMGFFPHEYPD
                     LTESTYWLLAISGAIGLFGSLILHELSHSLIARRYDIPITGITLFIFGGVAQMDKEPP
                     NATSEFRMAIAGPLASFVLAGVFYGAYALGQLLSLPLPVLAVLGYLGFINLVLAVFNL
                     VPAFPLDGGRMFRAMLWHWKRDLRWATHLASRIGSGFGFLLLFLGVLNILHGRPLNGI
                     WFCVLGLFLDRAASASYDQLLARQAFEGEPIQRFMATDVVTVAPDLSIRAFVDQYVYR
                     HLHDIYPVINHSQPVGCIHARHVNNLNREEWDHRTVNDLLIPLNDHNTIAPDTDVMQA
                     LSLMNRTGNSRLIVMDENRLVGIVALKDILKFLSLKLDLEGTR"
     gene            complement(26947..>27797)
                     /locus_tag="VLA60_13075"
     CDS             complement(26947..>27797)
                     /locus_tag="VLA60_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HSF10340.1"
                     /translation="AGRTEKEVYAQVGLPYLEPELRENWGEVEAGLHHALPHLVTLND
                     IRGDLHVHSKASDGHFSIEEMALAAQGKGYNYLAICDHSQHVTIAHGLDANRLARQIE
                     EIDQLNDTLKGFTILKGAEVDILEDGSLDLCDDLLQRLDLTVCAIHYKFNLPRAKQTE
                     RIIRAMDHPSFSILAHPTGRMINEREPYEIDMEQIMTAALERGCCLEVNAQPVRLDLN
                     DRHCKMAKDMGVKVVISTDAHRTTDLDFMRFGVGQARRGWLEPEDVLNTLRLNELKKL
                     LRRASS"
BASE COUNT         6130 a         7151 c         7951 g         6565 t
ORIGIN      
        1 gttggccatt tgttgagatc gactgttgtg ccttccggca cctggaaatc gttggattga
       61 atatgcatgg tcgcctttct atcagggttc attacgggac ccgctcaccc cgtgttcaga
      121 ggtgtttcgt tccttggcca caggacctca aagcgggcga tgacgtcgaa cagctcctcg
      181 tcgatgctac gttggcggcc cccctctcgt gtcccagtga tgttctgtat tgccgggttt
      241 cgtcccggca ggcgaggccc ttttgttccg gcaaaaggac ccaaaaccat tgacgccccg
      301 tctagattca ttagggtagg atggacgcaa gccgggaggg cggaccaaca tgcggagcct
      361 gtcctgagtt ttatcgaagg gctcaaacaa ggcccgccag cggatgagag cgtccatccc
      421 ttaggccaga cggcaggcgt cggaacatga aaaatgaacc tttcagggaa aaatatgatt
      481 gagcgaggaa gcatatattt tatgtggttc cattaccata cagggaagtc tttggtctga
      541 atgttcgttg ttgcccttct ttcatgattc gatgtggggg tcacctgcat ggcattccct
      601 ggcggctggt tttttggcca gcgcctcaaa accggagatg acgtcgaaca gctcctcgtc
      661 gatgccactt tggcgcaatt ggtggaagtg ccggttgagg tcctccagca gctcgtcgat
      721 gtttttctcg gcgcgttgca tggcggtcag tcggctggcg ttttcgctgg ccagagattc
      781 ggcacatgcc cggaagagtg agataaagaa atactcatga atgagcgccc gcagagttgc
      841 ggtactactg ccgatgacct ccggcagatt tcctgtcggc catgggattg tggtcaactc
      901 gctgcgccat gtttcgtcca gtggcagcag gcgctgattg accggcgcat agaccgctct
      961 ggaggtgggg cggttgtaaa agaggtggag ttcgtggatc tttccccggc tatgagctgc
     1021 ttcgctttcc aggagaatct tcccgacaag tggcgtaatg gccttgaccg aattcgggac
     1081 cggaaagatt ccttctaacg acaaacctgc gtctgccagg cgcgactgca cgcgctcccc
     1141 cacggcccat acactgtgat gtccagaccg agcggccagt gtcttgatcg cataatcggc
     1201 gacgacttca ttaaactggc ccaccagtcc ctggtcggaa ccgaacacga tcgcgccaat
     1261 cgcctctgca tcggattgcc ttttctgttc ccccatcaag gccatcggcg agtcttcccg
     1321 gaaacagacg cttaacccca gctccacggt gcgagcgtag tcggccaatg cgcttaccga
     1381 ttgctcgtat tggccgatgc tcgaggcggc caacgccttc atcgtgcgga cgaccgattg
     1441 gagatccccg gctccgccga tctttcggcg cagactcacc atggtgtcgc tcatgactcc
     1501 tctgggaatt ccgttccagg tggtggaggg tcagtcctat gcttcttttg atcgcgcaat
     1561 tgcccgagag tgctttcaag cattctggtc agcttctctg cagcgccgtc gacggcttgg
     1621 tccacggtcg cggcctgatt cgtgaccgcg acgggctggc gtccttcaag gcgggcttcc
     1681 atcacgcaac gcatgtcgtc tttgccgccc ttcttgtcgc tattctcatc gctcaggtgg
     1741 acctccaccc gggtgatgga atcgctgaat cggctcaggg cactcttcac gacgccactg
     1801 acctgagcgg ccaatgcctc atgaccttta atgtggcggt cagtattgat ttggacctgc
     1861 atgggattct cctttgtgag tgtggggggt tcaatgccgg taggtggcag ccagtcccgt
     1921 ccgtcccggc atcttctcgg ccggaatgac caggaccctg ccgcccatct tttcgaccag
     1981 ctcacccagg tcgtcgagca ggtcatcgac ttgcggatgg ctcaggtccg caaactcgac
     2041 ctgacccgtc gcactgtcca cccgtccggc aatctggcgg tcggcctcga tcaggagcgt
     2101 ggcgacccgc ccggtggcgg ccgcctgtgc aacttttgcc aggtcgtcac tgccaagtcc
     2161 cttggatttt gccactgcga actcgtcggt cactgtagcc atctgggact gatattgcgg
     2221 gtcgacaacc tgccaggcgc gttcccgaag ttcgtccatc tgcagcgcat cgggattgat
     2281 ggtgagtcct tccgccatca gaaacgggtt atggctgacc ttgcgaaaac gatgatgatg
     2341 ttccggtaat gccgccagga tcagtggcag gcccgacggt cgggaatagc gttctaatac
     2401 tgcgcggtcg actgcgcgaa aaaaccggtc tgcgtcgatg tcaacctcgt ccttttttcc
     2461 gccgtggccg tggtgcatgg ccccgctcgc ctgaccgacc ccaccataag aagccacggt
     2521 agaatggggt tcggtcagct catcccccag cgcctcctcg atcgtccggg ggaacccggg
     2581 gacaagatcg atctcgtcaa gcgcatggcg attcccccca aagagccgga tcgtgcgaag
     2641 gcttagtccg aggacctggt agcgatcaac cgattgaaga aaacgccgca acggcttggt
     2701 gtgaaagctg tcggccacga cgaccaattc ggcgaccggc cactgaagcc ggaacacatg
     2761 aaataagcct tgcgcggcca gcacggccag tccatcgtgt gtgtgggtcc agaagtcatg
     2821 atcgttggtc agggcttcaa acggttccag aaggaggcgg gtttcgacgg ccggatactt
     2881 ttgccggagc gaggtttcca gctccttgac aagattgcga aaccggatcg ggtcctgctg
     2941 attctcggga tggcgccgat gggtgggctg gtaaagcgag agacacggcg gccgatgagc
     3001 ggctaccaac tcggctatca attccctggt gagcagagtc gtcgtttgtg tcattcctgc
     3061 ctcctctttt gtgttcaagc attgtgaaac ttcaataata gcttactgac atggcttaac
     3121 cagtcgattt ttcgctccta tgaagtgact ttttgtcact tgaggcggcc agggccttta
     3181 aggtgcggcc gactgattgg agatctcccg cactgttgat cctgcggcgg tggctggcca
     3241 tggtgtcgct caagcctttt ccccgattgc cgccctggac gtggtgttgg gttgaaagcg
     3301 tgcgagcaat ttatgggcaa tctcgacgat ctttctccga tcccggctat ttcattttgc
     3361 ggcggtttca aaatcggcgc gatcgtggtc gtgggacgag gctttttatt ggaactcata
     3421 ctgcgttttt tatttctcaa agagcaatct tccgcccagt cgtcgcagcg tctccagcca
     3481 taggctgaca ccaaccagca agaggaggct ggcaataagt tgatgcatgc cggccggcgc
     3541 aaaccccatg accccgcgca ggaagggcac ggtcatcacc acaatgagca gtagtgccac
     3601 ggccccgaac atgcgcttga tccagggatt gttcgcagca aggcccatga tggcgggccg
     3661 agacagatcg cggttggaca aaaccagcaa gaacactccg agtaccagtc cggtgaaaac
     3721 tgccaggcga ctttccgttt cactgacgcc ctgcccgacg agaacaccgt gccccagcag
     3781 caggagtgcc gccatgccgg caccctggat gactgcatag gtcagattgg ctgtggcgaa
     3841 cggcgaggcg gaaacctgcc gtggcgggcg ggtcatgagg tcgggggatt ccggttccgc
     3901 ctcgaacacg atggagcagg ccgggtcgat gagcagttcc agcagcacga tatgcaccgg
     3961 catcaacagc accggccatt gcaggaaggc gggaaccaga accagcatga tgatgggcat
     4021 gtgcacggcg aaggtgaagc gcgtggcctt gccaatgttg tcatagatgc gacggccctg
     4081 tcggatggcg gcgacgatgc tggcgaagct gtcgtccagc agtactaacg cggcggattc
     4141 ccgcgccacg tcggtgccgc gctcgcccat ggcaataccc acgtcagcgg ccttgagcgc
     4201 gggggcgtcg ttgacgccgt cgccggtcat cgccaccact tcgccgtcgg ttttcagtac
     4261 ttgcaccagt cgcagtttct gctccggctg caggcgcgcg cacagatcca cgtgttgcaa
     4321 tttttcgcac agcgccgcat cgctgagtgc cgcaatgtct gacccggtca tgatttccgg
     4381 ccgctcgctc agtcccactt ggctcgcgat ggcacgggcg gtggcggggt ggtcaccggt
     4441 catcatgatg aggcgcaccc ccgctttgcg acactcggcg atggctgccg gcacttccgg
     4501 gcgaggcgga tcgttgaaac ccaccagtcc aagaaagatg aaggtaaagt cgtgttggtt
     4561 ttgcggccac tcactgacct gccagtcacc cttggccacc cccaatacac gcaggccgcg
     4621 ctcggccatg gttgtgacct ggcgctggat ttcgtcgcgc tccgcttgcg gcaggtggca
     4681 cagatccgcc accgcttccg gcgcgccttt ggtggccagc aaatggtgtg agggttccgt
     4741 gctggagaat acctgtgtca tcgcaaggat gtcgggagag aggtcgtact ggaacgcggg
     4801 ggtccggccg tcatgcacgt gctcggtgcc gatgagccag cgatggccaa aattttgaat
     4861 ggccttttcc atcgggtcga aggggtcaga gggcgtggcc agcatggcga attcggtgag
     4921 ttcgtgaaac gcttccggca ggctgtttgc gtctgtgttg cggaacacct catctgcggt
     4981 ggccagctcc gccacctcca tgcggttttg cgtcagcgtg ccggtcttgt caaccgccag
     5041 cacggtgatg gcgccaagtg cttcaaccgc cgacacgcgc cgggtgagca ccttttgctt
     5101 tgaaatgcgc caggcaccca acgccaaaaa gacggtgagg atgaccggaa tctcctccgg
     5161 cagaatggcc atggcgagcg cgatgccgga cagcaggctc tctaggaagg tacgaccgtt
     5221 ccacagccag ccgaggagta tttgcgcgct cgcaagcaaa agccccgcca ccgtcagatt
     5281 gcggatcagc ttgcgcgaag actgttgcaa accggaaggc tcttccttgg ttgccgccag
     5341 cgcctgtcca atgcggccca cggcggtcgt ggcgccgatg gcgcgcactt ctgcgagccc
     5401 cacccccttt gtgaccactg tgctcgcgaa caaaaacggt gtgccgtcac cgccgggctg
     5461 cttgaatgcg gcattcgccg cgccgggcga tttcgccacc ggcaccgcct cgccggtgag
     5521 cagggattcg ttcacggcaa gctgtccctc cagcagcagg gcgtcggccg ggatgcggtc
     5581 gccttcgcgt agcaccaaaa tatcccctcg cgccactgcc gcgccgggaa tgcgcatctc
     5641 ctgcccgtca cggatcacca gcgcgcgcgg cgcggagaga tcgcgcagcg actccagcgc
     5701 gcgctgcgtc ttgcgctcct gggccagggt gatgccgata acgacaatca cgaagccgag
     5761 cagaaataac gcttcggcgc gatcacctag tgcgagatag atgccacctg ctaccaacag
     5821 catgaggaac atgggttcgg tcagcacgcc gagcacaatg gcgaacattg acttcggcgt
     5881 gccgccgggc agcaggttcg ggccctcggc ggccagacgt cgggccactt cagccgaggt
     5941 caggcccggc accgatttgg tgaggcggct catgacgttt ccttgtgtgg cgcgttgtgt
     6001 tggaaaggcg tgagcgcgtt gcgggcgatg tcgatgattg tttgacgatc ttcgtcattg
     6061 agtttggttg ccgcttccaa tcgcttgcat acttccaatg ggatgtccag ggcggccttg
     6121 tgtaacgcct gttcggcctt catcatctgg tcaaggggca caagatcgaa cagtttttcg
     6181 atcaacgcca ggagcagagt gatttgagcg ggcacggaga ccggggcgaa ttccggctgt
     6241 ttgaggcatg cgcgaattct ccgtccgtgt tcgatgatgg cgcgggtgtc gtcatccagt
     6301 ctggcgccga acctggcgaa ggtttccagt tcttcaaact gtgcataggc cagcttgaga
     6361 tttcccgcta cggcccggta gaccgtccgt tgtgccttgc ctccaacgcg ggaaacggat
     6421 cgaccgacat cgatcgcagg cagcacgcct aattcgaaca gagagggcga gagatagatt
     6481 tgtccatccg tgatggaaat caggttggtc ggaatatagg ccgaaatgtt ctgtgcttcg
     6541 gtttcaatga tgggaagcgc ggtcagggag ccaccaccca ggtctttgcg cagatgtgtg
     6601 gcgcgctcca gaagtcgcga gtggatgtaa aagatgtcgc cgggaaaggc ttcgcgaccg
     6661 ggcgggcgcc gcaaaagaag ggacagctcg cgataggcgc gagcgtgatg cgtgaggtcg
     6721 tcgtagacca cgagcacatc gcgaccctgt tccatgaaat gctcggcaat actggtcgcg
     6781 gcgtacggcg cgatataggc gagtcccggc gggtcgttgc cctctgtgac gacgacgatg
     6841 gtgtattcca tcgcgtcttt ttctcgtagg gtggccacca ctttggccac accggatgcg
     6901 cgttggccga tggcgcaata cacgcacagc acattctgat cccgctggtt gagaatggtg
     6961 tcgagggcga tggcggtctt gcccgtttgc cggtcgccga gaatcaactc acgctggcct
     7021 cgtccaatcg ggataagtgc atcaatgacc ttgatgccgg tttgtagcgg caccgtcacg
     7081 ggggcacgat tcataatggg cggggccgga cgttcgatag gcaggcgttc ggcggaaacc
     7141 acaggcccat ggccatccag aggccgtccc aatgggttga tgactcgccc gattaatttg
     7201 tcgcataccg gcacgtccat gacgtgtccc ctacgttcga cttcatcacc cgcatgcaac
     7261 tgccagtagt cgccaaggag cacaacgcca atctcatctt cgtcgacgtt gaaggcgatg
     7321 ccatacacat caccgggaaa tttcagcacc tcctcaaagc ccacgcgggg aagcccggaa
     7381 accctggcga taccggtgga gacgtttgtg atcctcccta tctcgtgggc ctccattccg
     7441 ggtttgaagt cctcgcgtgc gtggctcatg ccggcgaacg ttttgtcgaa aagactgtgc
     7501 aggttttcaa ttgggttgtt cattggcgtg gcgttcggga tgtgctatgg cttttgttgc
     7561 cgcttttgga cgcatgttca ggaggtcgtc gacgcgtttt tgaagcgatg cgagataatg
     7621 tccgatgctc caggcaatct tgtgtccatt ggcggtgagt tcgatgccac tcaccaggtc
     7681 cggggcggtt tcgaactgga cttgggtctc aatggcgagc acctccttga ccacctcttc
     7741 aattgcggta cgctgcgccg aaggaaggtc gaacgtgctc cgtataatcg ccggagtgga
     7801 cgcggcctcg aacgccgacc gtagcgcacc cttctcctca ctgctcagtt tgtgcaatcg
     7861 gcggatgaag gcgtcggcca tctgttcttc cagggtcgtt gtggtgaggt ctgccaatgt
     7921 ttttcgcgcg atggcaaaga cttcctctcg tgttcggcgg gtgagttctt cactgaggct
     7981 ctggtgttcg ctcctcagcg cctcctgcca ctgggcccgc aaggtgtccg actccttccg
     8041 cgcgttgtcg atgagctgtt gacgctcggc tgtggcttga ttcaccgcgt tggtcagcag
     8101 tgccgcgcgc tcctggtcga attcctcgtt tttgcgcttg aatgcgtcgc gttcctgtct
     8161 ggcttcggcc ttttttgcgt cggcgtctgc aagttcggcg gcgatccgct tttccctcgc
     8221 atcgattgca ttgaggatgg gtttgtacag gaaatgcttc aacaaccaga ccaggatgag
     8281 gaagttgagt gcctgcgcaa caacggtgaa ccagtcgatt aacatcgtct cactttccgg
     8341 cggcctgggc gaggacgtgg ttccagaacg ggttggcgaa gatcagaatc atcgagacca
     8401 cgaaacagta aatggccgtg gattcgatca tcgccaggcc cacaaacagc gtccgggtga
     8461 tcgtgtttgc ggcatcgggc tgctgggcca gcgaagtcaa ggctgttgaa accgcccttc
     8521 cttcccccag cgcgggccca atacagccga tgccggtggt gaggccggct gtgataatcg
     8581 atgccatagc gatgagcgtc atgctgtcca tagagtctcc ttttggtgtg gtgacgtgtt
     8641 tgtcaatctt gtgctccgga atccggttcc aatttcattt gacgtgttcg tgtggcggcc
     8701 gcaatataca ccgtggccag gatgctgaag atataggctt gtaccatacc ggtgagcagc
     8761 ccgagcaggc tcatgacgat cgggaagatg aagggcgtga tggtcagcag aatgccgaca
     8821 atcatggctc cgctcatcat gttgccgaac aagcgaacgg ccagggccag cgtgcgggaa
     8881 agttcgctga taatgttaaa cggcagcatg atgacggtcg gtttcagata ggactggaga
     8941 tagccgccga ctccttgctc ctcgatgccg aaaagcggta ccgcgacaaa cacacacagc
     9001 gcgagtgcca ccgtggtcga gagggatcct gtcggtggct cgtaaccggg aatgatcgta
     9061 cagaggctgg ctgtggcgac gaacaggaag atcgtcccca gaaagccgag atacttgcgc
     9121 ggctgactca ggcccacctc ttcaatttgt ttcccgatgg cggtgacgac gatttccaga
     9181 agattctgcc agcgggaacg tgtcaggccg gtggacaggt ggcacgtaat gactaatgat
     9241 ccgacggtca gcacgaacat cactccccac gtgaacacaa ttgtggcatt cagcttgata
     9301 aacccgtaat gccagaaaat gatgtcgtcc gggctaaggt gcatggctgg gctcctgcgc
     9361 cggacggctt ggattttcgg ctaagccggt gagccgggtt acgatcggac gtgcgatggt
     9421 aaatccgacg aggcacacca gcagcctttc ccattggccg tccgagacca catagaaacc
     9481 agccagggtc aggcaggttc gtagtagcag actgccgatg aaccaaatgg ccggttgtgt
     9541 ggacgatagc cctttccgaa ccgtccacca caggcccccg aaaaacatgg ctcccagcac
     9601 tattcctgtt accagcgcag gcaccatcat aaagaactca ctcatggtga ttctcctgtt
     9661 cggcacgtat ttcacggtct tccttggtca cccaatgcca cgcgttcatg cagccgatga
     9721 tcaggccgat gaagagcagg gtgagcgtcc atgaatgggg tccgggatac tgtttatcca
     9781 gccagagacc gagtgccgcg ccaagtagcg tcggaatggc caccgaccaa ccgatcagcc
     9841 ccatcatccc caacccaaac cagatggtcc gcgtcgtgtg acgctgcgcc ttgagtttgc
     9901 gcgcagcttt cgtgccgacc tgtcgggcca aggtcgtctc ttccctgaga ggtttttctt
     9961 ccgggtcctc agtcatggcg aaaatccgcg aaacggcgca caaagctact ttccaactta
    10021 gccatcaccg agcggaggct ttgctcgtgt tcgtccagag tcagaaattc acgttccacc
    10081 gcctcgcgca attggtgaag gtcggtcccg gcaatggcgt tcctgacgga gaccaacacc
    10141 tcccggccat ttttgaccag cactccttca tcgagagcca catattgctc atcatccccg
    10201 ttggtttcgt aaatcagaat tcccggctcg agtgccgcta cgcagtcaag gcgctgaggc
    10261 aaaatgccaa acgcgccctc acgggtccct gcgacgatac gtgtcaccgc gtgctgttcg
    10321 gcaaaaatcc tgaacggcag aagaattttc agtgtcatga gcgtcggctg catgttcgac
    10381 ctccggttgc ggttaggacg gtggtttcga cttaccgttc ggatttccca ttgagtttcg
    10441 ggtcgtccga tggttcgttg ttcgactctt gctcgtcttg aactgccggt ttgcccgatt
    10501 tcgctttttc cttcgcttcg tcaatccttc caatcatgta cagcgtatgt tccgggtaat
    10561 ccttaaactc gtcccccagg atgcgttcac aaccgtccaa ggcgtccttg cggctgacaa
    10621 gctgtccttt gaggcctgag aactgttccg tcgtgaaaaa cggctgggtg agaaaccgct
    10681 caagacgtct ggcgcgagcg accaccttcc ggtccgccag agagagctgt tccaagccaa
    10741 gcatcgcgat gatgtccttg agctctgcat attgcgcgag tgtccgcctg acgtcttgcg
    10801 cgatgcggta atgccgttca tcaatcgtgc ccggcgtggc catttttgaa ttcgactgca
    10861 agggatcgat ggccggatag agcccctcac tggcccgttt gcgcgaaagc acgatggatg
    10921 cggaaagatg cgagaaggtg tgcacggcgg ccgggtcggt gaaatcgtct gcaggcacat
    10981 acacggcttg aatcgacgtg atggccccgg aatcggtgtt ggcgatgcgt tcctccaacc
    11041 gggacaattc ggtccccatc gtgggttgat agcccagacg tgatggcatc tgccccatca
    11101 gccctgaaac ctccgagccg gcctggatga accgaaaaat gttatcgatc agcagcagga
    11161 cgtcccggtg ctcgtcgtca cggaaatact ccgccatggt caacgccgcg tggcctacac
    11221 ggaaccggct gcccggtggt tcgttcatct gaccgaacac catcaccatg ttcggcagca
    11281 caccggcttc cttcatttcc cggtagagtt cttcaccttc acggcagcgt tcgccgatcc
    11341 cgcaaaaaat gctgactccc tggtgatgcc caatcatatt gtgaatcatt tcggtgagca
    11401 agacggtttt gcccacgcct gctccaccaa acagaccggc cttgcctccg cgctccaaag
    11461 gcaatagtac atcaattatt ttgatgcctg tctcgaagat ctcggatttg gtggagcgcc
    11521 gggccaacga aggcggggct cgatggacgg tgcgatattg aatctctgtc ggtggcggct
    11581 cccggtcgat ggaattgcca aatacgtcga acatgcggga gagaatgcct ttcccgacag
    11641 gtgccatcag cggtccgcct gtgtcttcta cgaccatgcc gcgcgcgagg ccttgtgtgg
    11701 gcgtcagcgc aattccgcgc acgcgatgtg tgtcgagttg ggccaatacc tcaatggcga
    11761 ttcggccctc ctgtcctgtg cgcaacaacg agtagatggg tggtaaatgg tcctcgaacc
    11821 ggatatccac cacgctgccc cgcactgaga cgactgcgcc gagattcaag tgattgatgt
    11881 tagtgtccat gtgggggctt cttggatttc cggtcgctgt ggatcctcca gttggttggg
    11941 cggtcgcgct tcagagatgg atttgccttg atctttgatg gattatgcgc caaatggacg
    12001 cgctcctcaa ttcaacctct taccggtttc atgagtcaat atgttcatgt tcaagaatgg
    12061 tgcccgcagt gaagactttt tgcccgtaac atatactcag gcatcttaga gccattgtag
    12121 agaggttaga aacctgtttc tacaatgtta tcaagaatca catcgccttc aacagaggaa
    12181 aggggggcgt agttttggca gcagggagac ggtgattgaa aggaaaaagg aagtcgacta
    12241 tggaaccgga tctgagcgac agcatcgcac ccgttcatgc ttcgagagct tcagcacgaa
    12301 cgggtggccg tgacgctcat ccgcgagggg ggagagcccg ttcgctctgc ccgccgcgcc
    12361 cggcgtggca gtcgaagagc ctgtcccgag ggagagtcga aggggaacgt catacctaac
    12421 cgccattttg tggtttattt gcgtgaaccg gatgagggtt tggtgcgctt cttgttcggt
    12481 tccgttttga gcggtttgga taccgcatca acatcggtga agggctgtcg tttatgataa
    12541 ggatgcggga cgcaaagcac tggacatgtt acggtgcgaa ccactttttc ggccgtactg
    12601 ccaaaaaaga tcctggtaat atcccctgtt tgcccgccat aactgcccat gacgacaagg
    12661 tcgacctgtt ctgttcgtgt catacgggca atttccataa agggtttccc ttcgctgagt
    12721 aagcgcgaaa tttttatccc tttggcttca ggcaacgcca agagacctcg tgcgagaatg
    12781 cgggcctggt ggtgtaatcg ttttttctgg gctttctcct ccgaaggcag cgcgagcccg
    12841 agagcgttga gggcatccaa agatttggtg tcgatgacat gaaggagaat aacctctgat
    12901 tgaaaattcc tggcgagacg aaggccaacc aggaacgctt ctcgtgagca gggagcaaaa
    12961 tcgacgggga tcagaatttt ttgaaacaga ccgtcaccca tgttgttgac ctcgtgatgt
    13021 ggttgtcata gcgtagatat ttacgataat gcatgagaac tcagcaaaga aaatgccaga
    13081 ccatgttttc ttcctgagaa ttgggtttga gtgagacgta gacgatccaa aacgttggct
    13141 tctggtcaat taattttatt ggtctcctga aaaaagcgga aaaaagctac accatgatga
    13201 aggaaaatag tcccaaaaaa gggcaggtgg aaatagggtg aacggttatg aggttttatt
    13261 ttatgtaaat ttcaaacttc cggccagaaa aatatcgggc aaatactttt ccacttggca
    13321 ggattcctgc gaggggaaga gtgtgtcgtc ataagcaatg gacttgtgtg aagtgttctg
    13381 tgtgaatgag attagggaga tgagccaatg aaaatcctat gtgcggttga tgggtcggag
    13441 ttttctctct gggcgcttga atgtgtcggg aagttgtttc gccattcgat gaaggagttg
    13501 gtgttgctgc atgtggttga cccacggatt ttgagaggcg gtggggtgcg gcgatcccaa
    13561 aaggatgagc cgaccaccaa agatatttcc aaaaaactgg aggcagcggg tcggaaggtc
    13621 ctgacggatt gtgcgcaccg gatggaggtg gtgttaagcc aggccgccac caagccgttt
    13681 gcagcaatta agacggcctt ggtgaaaggg catgtggctg attcgattat tacctatgcc
    13741 gagcggagcc ggcctgactt ggtggttgtg ggatcacgcg gaatgaacga tctcccggga
    13801 tatctgcttg ggagtatttc gaggacgatt ctgacccatg gcccctgctc cgtcctgaca
    13861 gtcaagagtc cgctcgacgt gccggcttcc gtcctactcg ccttggatgg gtccaagcca
    13921 tccaagtttg cggctaaccg ggtgaaagaa tggttgtctc ctgaagaggt aacacttcac
    13981 ctggtgtcgg ttgtgcccga tatcttgacg gatgcgtcta agaagctatt cccgaaatca
    14041 cggctgaaga ccctggtggc acctcttcag cagcatgctc aggaatgcct tgagcaatat
    14101 cgagaattgt ttcttaagga agggtttcaa gtggtgggaa aacgtcttca ggggagtcca
    14161 cgggagaaaa tcgtcgaatc ggttcctgtt tgtcacgccc aattggtggt catgggatcc
    14221 aaggggctta cgggtgtgga acgatttact atgggaagtg tctcggaatg ggtgagcgcg
    14281 tatgcgcctt cttccgtgct ggtcgtccga cattaacgca atcgcatgaa tgtcgaatgg
    14341 gctgtcgcct tcatggatat ccctgaagag agtctggcgt tacgcatcat aaaggagggc
    14401 aattgttgaa gacaggaaag acagaacgat cgcatgtgac gtctgcaaag acaaccgggg
    14461 cgtgggtggg aaagctgctt ggattcgtat tggcggggag tatggtttcc acgggagcct
    14521 ttgggaagca tctggatttg tccgctcccc atgcagacat ttctttggac tgtcatggga
    14581 aattccttgg gaagcaggta cccgtcgaga cgctcaagga aatggtagcc gggcatgccc
    14641 gatggcttgc cgatcatcaa gacccgaaag ggacacaggc caatttctgc ggtgcagatc
    14701 tgtccggggt cgaatttcgt gaaacagact tgagtggtgc caactttcaa ggtgccaatt
    14761 tgacccgtgc cgtgttcacc gccgccaaat tagtccatgt gaattttcac aaagcaaatc
    14821 tcacggaaac ctctttgtcc tcggttgatc tgcagggagc caatgcctca ggcggcacat
    14881 tccgcctggc ggattttcga aaagctaata tgacccaggc caatttcgcc ggagccgatc
    14941 tgcgagaagc gaatctggca gagtcccgtt tccgcttagc catcttgcgg ggagcagatt
    15001 tagaggacgc ctatttggtg agggcggatt ttgaaatggc caaccttgcg gaagcgtctc
    15061 tcattcgagt ggatgggcgg gaagcgaatt tccaacgagc gttactcgcc aacgccaaac
    15121 tgatcgatgc tgatttgaac cggagcaatt tacaggaagc ggatcttcgt tcggccgatt
    15181 tatcaggagc ggatttaggg ctggctaatc tcaatgaagc catgcttccg catgcaagac
    15241 tcagtgaagc gaatttagac cgggcagtgt tatttaaagc caatctcgaa ggggccaatt
    15301 gcgccggtgc gagctttcag aatgccgatg cgctcgaggc aaatttagtg aatagtaatc
    15361 ttttgcgtgc gacactggac gaagcctatc tcgcaaaggc gaatttgcag ggggcaaatc
    15421 tggaaggcgc cgatgtctcg tgggcgaatc tggttgaagc ggatctttcg cagagcaatc
    15481 tggaatttgc agtgctgttc cgtaccaatc tgcaagaggc caaactggat ggggcgaggt
    15541 tttacggggc cgatcttcgt ggagcgaatc tcgaaaaagc ctctttgcag ggcacacatt
    15601 tggagggtgt ggccatccga tatgcccacg gattaaacca ggcacaactg aatcagtctt
    15661 gtgtcgaccc acgaacccgg ttgccggaag gattggtggg accatcccca tgcctgcgct
    15721 gagcaggaaa atttggacct tgcaaaacgg tgaagggggg ggcaacgaac atgctcaatg
    15781 tctcatatac aagtatagag tgtgaatcca tgcaaccaga cacggagtgt cggaatgttc
    15841 accctatttg agccgggagg catgcggatt ccctatgtgt cgggtgtgtt ccggcagaga
    15901 aatgtcgaga aaatttccaa tgcctctcca atcggccagg tgaaggttgg acaggaaaat
    15961 cctccctcga tttctcagaa gaatgcaggc ccttcttcct tgcaggcccg cttgtatgag
    16021 caggttaact cgttcacgca atcttctcga gcaaagattt ttgccaggga catcatgtcc
    16081 tcccctgtcg tcacgctttc tgtgacgtcc tcacttgccc aagcctggga tgtgatgaat
    16141 tccaggcgat ttcgacacat tcctattctc ggggcagatg acgtcgtggt cgggatgttg
    16201 tccgatcgcg atctgttccg gggaaccatg gagtcggttc tttcagggac atcggagtct
    16261 catacacaag ccgggggttc tatccaaagt ctggtcagtc gcccggtcct agtcgcgtcc
    16321 ccggaagccg agcttcgcgc actggcgagg gtgttgttgg aggaacacat tggcgcattg
    16381 ccgattgtgt ctgaagccgt gggcttagtg ggcatgatta ccagaagtga tattctgcgt
    16441 accctggtcg cccaccctga ctttgaccaa tgggtataaa cgaaagagat tctccgccca
    16501 agggagagga agcggacagt tcctctctta tccttttgct gagttaaagg aggaaacgta
    16561 tgcttaacca aggagagaaa ctgttaatcg tccatcgccg actatttgaa aaagatacgc
    16621 cccggttttt tgtgggagag gtgctgatct atgaagccgg aatcgtcaag gtgaaagggt
    16681 atacgtttgt caaagatctg tttagtggaa acatgcaaaa gaagtccgat cttcgcacaa
    16741 aagtcatgtc gattgtctca gggaccctta ttgtgtatca attgccggtg acggctctct
    16801 tggattcgat gagatttagt ctggatcagg atggagggtt ggtgctcacg gatgagggag
    16861 gattttcgat ggatgtgtcg gaatccctct ataaacacga aacgcatcaa taacaacacg
    16921 tccttggttt ttcctctgcc aaggctcgct tccttccttt ttttttgtct aagcgttcag
    16981 cctcctatgg ccagcccatg gtctctgcaa gggagtgttg aataatgaag attttgaagg
    17041 gcaaatttgg attagattac tcatcaaagg ctccttgtcg gttcaaaaaa gtccgtccag
    17101 caaggccgca gacgttttta cgcacggagc gtacgctgag aacgtgagca cggaaaaatg
    17161 gcgagaacgc cgctggcggc ttttttcaac agacccatgc aaggatttgc atgggtgcga
    17221 ttctctgccc tttagggaga gattggccaa taagaggcag cgggaagaaa atggcgtgaa
    17281 tgttcagaaa ggattagcga ccgcctcaaa ggtgctacgg aattgatcgg gggtcatatt
    17341 taacatttcg gcatactgtg taaccgtttc catgtttgcc aaatcttccg gttctaatcg
    17401 ggccatatag gcacgggcaa tcttatagga ttccgaatca acgtcgacca tgcgcacgcg
    17461 ggtgcggccg gtggcggagt ccacgatgtc gctaaacgga aggggggtaa agcgtccgtt
    17521 cacgatggac accatggcac catttcctcc atccagaaga aattgggcgg cgcaataccc
    17581 caagtcccgt gtgtattcca tgtcgaacgg aatcgggtcg gcgcagcgaa gttcatagcc
    17641 gatatttttg gacacgatgg tgatcttgat gccagagtgt ttcagctttt ggctgacggt
    17701 ttccttcagg acctttccga tatccagttc cgccagtcgg atattgccat gttcatctcg
    17761 ttcaactccg cctaagaggg caagatcgtc ctgatccaaa aattccgaca gtccttcagc
    17821 taatacggcc acgccatccg agcggccctg gaccagacgt ttggtcatgg ctcccaccag
    17881 aatgttggtt aagtgaccaa gcttgagtgt ggtccccttg aattcctcag gaatcagagt
    17941 gagggtcgcc cccgccgctt taccgatgcc tagggccagg tgtccggcct tacgccccat
    18001 ggtgaccaca aaataccatc gggatgtggt atgggcgtca atcatgagat ttttgaccag
    18061 ctcgactccc atgtgccggg ccgtttgaaa tccgaaggtc ggcatgccgt ggggaaggca
    18121 taaatcgtta tcaatcgttt tgggcacgtg aacaacgtgc aatcgcccac tggccaattc
    18181 cgccagtttt aatgcggaaa aggccgtgtc atccccgcca atggtgataa ggccggttac
    18241 atccaatttt tggagggagg tcaacgtggt tgataaatct tgagggcgtt tggttggatt
    18301 ggctcgagat gtgcctaata acgagccacc acgaaagtgc aagtggctca ccgtttcgcg
    18361 ggtgagaggt tgagtgtggg aaaggtcgct tttcatcaac cattggaatc catcttcaat
    18421 cccaagcacc tctaccccac agagacgact tcgaattgtg gcggcttcaa tgacactgtt
    18481 aatgccgggg gcgggtccgc ccccgactag aatggcgaat tttttaggag atggggaatc
    18541 catggtgtcg tttctcgcgc taaaaattat agcattccat catagggaga ggcccttcca
    18601 gtaatcaagc cggagcgtgt gaaattggat cacgaatcaa gagcaccgaa cagggggagt
    18661 ggtgaatggt cgcatgagac acactcccat gggccagtcg ttgaatcggt gaacgaccgg
    18721 gagtacccat gaggactaat tggggttgaa gccgtgagat ctgtcgttga atgactgagg
    18781 aaggcgcccc tggttcgaca atactttcta cggaatatcc caggcttgag agtttgtccg
    18841 caacgtcctt cgttaaacgg gctcctacat cctggagttc cttcttccag gagtccggta
    18901 agagtgcgcc catcgggaaa acaggctggg caaaggggat gacatgcagc acggtaacat
    18961 gaggagtgcc tcgaaaaggc ttctgggata aaaactcaat ggcccatccg gcatcaagag
    19021 gatattcgac gagaaggaga atgtttttga acgggacaac gggagccttg acgagaagta
    19081 cgtgacaaga tgcgtgagtg gcgatccggt gcgagacgct tccaataaga tgttccttga
    19141 tggtgcctaa cccgcgcgac cccatgatga tgaggtccac cgattcgtgc tccgtcatcg
    19201 agagaatgac tgaagcggga atcccaattt caaggtgttg agagaccttc ccgcattccg
    19261 gtggcaaaag ggaagcggcc ttttcgagga ttcgtgcgcc ttcttcccgc aacgcccttt
    19321 ctgcggcccc ggaaaaatca tgaccgacac tcatccctgt acctggatag gcgagttggg
    19381 gtatggatat ggtgtggagc agcagcacgc gttccggagg agacaaagcc tgtacccctt
    19441 gaacagcctc ctccgcctgt gacgaatcat ccacaggaat caaaatcgtt ttgtatttca
    19501 ctggtgcgtt ttgggttttc atgtgtcctt gattttatgg ggaccccacc agactgtagg
    19561 gtgaggttcc gtgtaatctg gtagaagaat ctgttgtcgt atcctggcat ggtttagttg
    19621 acgctttatg agtggggaga cctgctggtc ttcaaccttg gagcatatgg cattcaagcg
    19681 ctaaccgcgt caaaggatca tgcctgtgga gaacctcaat gtggtggacc gacgttcctc
    19741 tatgattgat gatcgtttcc agtcccaata aatctggcga cagttgttgt tgagttttgc
    19801 ctcgtggacg aacgattccc ggctcagaac actccagaat tcatcctcat tctgttcttc
    19861 ttgccgggga gtttgatcat caagttgaaa ctccgcttct ttatgatctg tgtgatgagg
    19921 gatctgcctg ttcgtgaatt gccggattag agcgtgaaag cggatgatca ggtttttggg
    19981 gtgggcatgt attgattccc aaagcttgcc acgccgggca gtatccaata ataccggtca
    20041 acaaggcgat aaggccgaca atcatgagga tgagatctcc ccatcccggc aagaaagaaa
    20101 atcctgataa ggcaaggagg agaaacccta ctccgacgcg aatcactcgt tcatggtgtc
    20161 cgatgttacg atattttttc atatcacacc tcttcaaatg gagaaagcca attgatttgg
    20221 tcccttagtc taagtcgcca ctggcgaccc gcactgcgcc caatacctgg ttggcgttga
    20281 aaggacgtcc gataatttga atggcaccca gttgactggc ttctggagcg aggctttggt
    20341 tagattgccc tcccaatacc accacttttc ctgagaatcc cttttcgcgg agttgacgta
    20401 aaatttccag accgctcggc tgggtcaaat ataaatccac caccaccagg gaagggttat
    20461 ggcataatac ggattcaata atattttccc cctcatccag caaaagaaaa gggtcgcctt
    20521 tttgggttaa agtctgctgt aggttttttc gcaagatggg gtcgatagtg gcaatgagta
    20581 acatacaaaa gacatccttt atggagatat tgtacaatct ggaaacttca aaacctgtgc
    20641 caaaatgcca ggaagagatg actttgattt tttaggaaag aaaattttcc aacattggaa
    20701 agtggcgcaa aactcctcat ttcagcttcg gagaggtgtt gcgcttggcc tcagtttcac
    20761 gagttttaga gtcttacttt tatgggtatt tcattttaaa aatgtgtaat actcattcaa
    20821 ttcaattgac gacaacaatg agaatcttgc ttggggtagg gaaataggtt ggtctgatgg
    20881 gctgttatcg ccacacgtgt cgtcatattt ttcaaaagga gtcgaaagtt catgagggcg
    20941 ttattagtgt tcgtttgctt cttgggatgt tcgggaaccg gatgggccaa agacattgaa
    21001 ttggtcgccc tgggggtgag aggtggcatg aactttaaag atgccggttt acagcctggc
    21061 gagaaggaag attttgaaca atttgacgtc tttggcatca tggggcttcc ctggaggtgg
    21121 gaaggtacgt ccgggtggga ggtgcgttct caagtctggg gtactgctgg tgcgttaagg
    21181 ggagccgggg atcttggatt tatcacaacg ctcacacctg gtttagcttt aaaaattcca
    21241 agttggtggt tgattattga tggtggagca ggaggagcgc tcatcagcaa gtggaagttt
    21301 ggccgccagg atatcggtgg tccgtttcaa ttcattgcac acgtgggtgt gggtattcag
    21361 ctcccaggac acatggttgt gggctggcga ttccatcata tgtctgatgc gacaatttat
    21421 ggaaagagcc gtggcgtaga cttgcacatg cttgaactga gttacgcttt ctaaatatgt
    21481 tcatggtctt cgtctatggc tctttcagca agcattggtg aagagatcgc cgtctggccc
    21541 ctttccccat gcatgacgct ctgtcttgac ttcctccagg agtgaaagaa gacaggcggg
    21601 tcagggtgcg ggaaagagga tgccccctgt gaaccgaaac aaattcccac gagactgaat
    21661 ttcgaggagg atgtgggctc ctcgatgttt gaaaaattag ctcccctggc tgctagcctc
    21721 catagacatt aatcaaatgg ttatcaatca ggtgattcaa taagtctgtg cgggagatca
    21781 tgcccagaag cttgccatca cgtacgacgg gaatccggat aatctgatgc ttgatcatgt
    21841 gtttgacgat ttcctccgct gttgccgttt cttcaaccgt caccgggttc atggtcatga
    21901 cgtcttcggc ctttagcccg tgcaaatcct tcccatcggc cagggctttc aacacatcaa
    21961 attcggtgac cattcctacc agatgattgc ccggttcaat gacaggtact ccgctaaacg
    22021 tgccggaatg gatgaggatc gagaggtcac gagcactata ctgacgggtg accgcgacca
    22081 tacgtgtttg cataagatca cgggccttaa tgtgaagatt cggtgtagcc atgaaacctc
    22141 ctaatagatg aatagaaatt ccttatgggg cgatcctgta taggaccata tggacgtgta
    22201 tattaaatct cgcacagtgg ttctcgcaaa acatcgggct gttcgatttc ccggttcttc
    22261 tcgctattct ctctgtcacc atcttctctc tgctattttt aaatggatcg gacgcgaacg
    22321 atgactatac aaaatagcga gggctaaggt ctcatgcagg taggaattca gggaaaaaga
    22381 ttgtaaacaa atcagggctc acccggcacg ggccatgtct gaaagggcat tcggtaggcc
    22441 gccaatcatg attcctgact catgaattgg aattctggtt ttggtgtttt gaaggtttaa
    22501 ctgaatctgg cagaatagag atgttctgaa caaaaatcat cataccttgt gaaaagtgag
    22561 actactctct caagaaagat gtccaggtga gtgagatacc ctatttttta gggatggcgc
    22621 cctcctaagg cgggggaata agcatgtctg ttatcattca gcgatttgaa ccaggaacct
    22681 tgcaagccaa ggtgcaagtc agaaccgccc acgccatgca acgaggcgcg attgttacga
    22741 ttcccacccg tttcgagttg gtggaacaag ccggggtcaa ttttctcatt cgaattgtct
    22801 ccaatctggt caggaaggtg caggcaaacg tcgatcaacg gcatccttct aaggctgtta
    22861 acacagatca ggacccattt ctgccttatg atcaggatct gtttgtagcg gacatttccg
    22921 atacccatgt gtgtttgttg aataaattta acgtcctgga tcatcacatc ctcatggtga
    22981 cgcgaacgtt tcaggaacag gaatccttcc tcacgttgag tgatatcgaa gccgtgttat
    23041 tgtgtttgac cgagttcgaa ggattggctt tttataacgc gggggaagcc gctggcgcga
    23101 gtcaacgaca taagcatttg caaatggtcc cacttcccct gaccgctgaa gtatctcatc
    23161 ttcccattga acctcttttg gaaacagccc gattcgaggg gacgattggg agggctcccg
    23221 ggctcccttt ttcccatgtg ttggtccgaa tgaatccgga atggattgct tttcccttaa
    23281 aaggggcaca agagttgctg aagcaatatc tgcatttgtt gcagactctc ggcctggcgg
    23341 acggtggaac gggagaggaa accagaagcc ctggccctta taatttgctg gtcacacgac
    23401 agtggttgtt attggtgcca cggtccacag agtgttttga agacatttcc gtgaatgccc
    23461 tggggtttgc cggaggcttt ttggtgaaga atgaggcgca gcttgatcga ttgaaaacct
    23521 gtggaccaat gaccgcactt cgtcacgtgg ccttgtgcta ggggaatttt cccatgttcc
    23581 cctcgtgagc gcccatgagt atactgaaag cctggtttgt ggtgatgtga gtgtctgttg
    23641 tctttaatac tcaaatcgag agggaagaaa aaactatgga tcttcaaatt gacagtcgaa
    23701 atgtgacaat gaccccacgg tggaaaatgg agattgaaag tcggatggcc gatctccagg
    23761 ccggccatga tgatctgatc cacgggcggg tgacactcac aaaaaacgcc catcacaaaa
    23821 aatcccaaaa tgtggccgaa gcccttgtgg tcgtcacgat gcctggacgg cataccctga
    23881 cggcgagaaa agaggaaaag acgttcgaag aggctatccg tgcggcgttc tttgccatgg
    23941 ccatcgaggt gaagaagttt cgtgacaaac gggcctccaa agaaattcgg gtcccgccta
    24001 ttccgttacg cggagtgatc tctaaattat ttcccaaaga aggatacggt tttcttctcc
    24061 aggacggagg aggcgaggtc tactttcaca aaaatgcggt acacggaatg aaatttgagg
    24121 agcttgagga tggggtggag gtctccttca acgtggaaga tggggagaag gggccacagg
    24181 caaccacggt taatccgctt cccagtgtcc cgggagccgt cgataaagct gccttttctt
    24241 gaagcggttt gatcaagaaa ggcattgatt tcagaggaat ttttcctctt ttgagaggaa
    24301 gcttggtgtt tgggagtggc aggaaatttc tgctctgtgg aaatgcgtgt gtggattttt
    24361 gcacattgaa tcggtaaatg atattcatgc acgtgtggtg tgaccgtgtt gggctcccag
    24421 caaattccgc agagggattt ttggcaaaga atacgatccg gcttaggggg cttgggcggc
    24481 taaaagttcg actcctgctg ttcaggcgat cacatgtagc cggacttctt tcagcgagtg
    24541 ctggttcccg atctgtccaa cccggctagg ccatttcagc accaacccca gaccctgctc
    24601 ttcaagtgcc agataaccgg gtcacaccaa agcccgaggc cgtgtggtgc ggtcgggctt
    24661 tgggtggttc tctgccgggc gggctttaag gtgtacgctt ttgtaatatc gggggtcgag
    24721 gactttgtaa tatcgggctt aggggcgccg gtctattcac agtcggattt tgtgtttccg
    24781 gcctttgaat atatgtggag catccctgtg agttctgtcc agcaaagcaa tctcgtgagg
    24841 ggtttaaggt ctgagggtcg atccttcgct caagcaaatc attggggttc agaagaaata
    24901 gcatcaagaa gtgatccagt gaggtcgcct cacccgatgt cacacctccg gctccgggtc
    24961 ccaacacata acctttgccc aataactttt gcacagctgc tgcacaatcc agccctctat
    25021 tgatttccac atcgacggtc gaagacatat tatcaaccac aaaagtgttt gtggcattct
    25081 cgacgcaatc caaaagaaca acattccgat tgaaaaaggg gatcttctgg gcctcagata
    25141 cctccccggt tagaaagact ccggtcagac ctccaagtac cagcgctatt ccaaaaacgt
    25201 tcttgtttgg tgaaaggtgc ctcatttggg tcctccgtgt gtgtaattgt aagagctatg
    25261 ccgagacatt ccgtaacctt tgtcacttcc tgcttgtgat tgaggattgt tcttcttcgt
    25321 ctttcagccc tccttccggc tagtggatgt gttgcgaaaa caacctccgg ttcaaacgtg
    25381 gagtccctcc tttgatgaag ttgaacaaaa aaatggcctt tgaggctttt atcatcctca
    25441 aaggccctcc attgccttag cccaatgtgc tcaaccacct atcaactgat cctatgatag
    25501 gcgagggaaa tttttaccac aatttgaatg agaatgtccc accccttgtg ggcttacggg
    25561 gtgctggacg cgacagcctc agagacaggc gccataacaa tctgacctgg gtttaaaata
    25621 ccggatggaa agtcgccggg gatcgggatc accatggcgt cgtgttaata acgatagtag
    25681 aggaaaactc tctggttgct taggacagga atacgcagaa ggtcaggaga gcaatcattt
    25741 gacagatgtc cccggtttgc tgagggggga gtttcaggct atcgggttcc ttccagatca
    25801 agctttaacg aaagaaattt caagatgtct ttcaaggcca caataccgac gagacgattt
    25861 tcgtccatga cgatgagccg gctgtttccg gtccggttca tcagtgagag ggcttgcatg
    25921 acgtcggtat cgggagcaat ggtgttgtga tcattcaacg gaatgagaag gtcattgacg
    25981 gtgcgatggt cccattcttc gcgattcaag ttgttgacgt gacgtgcgtg aatgcaacct
    26041 acgggctgtg agtggttgat cacaggatag atatcgtgga ggtgccggta gacgtactgg
    26101 tccacgaatg cccggatgga gagatcgggt gcaaccgtca cgacatcggt tgccataaat
    26161 cgttggatcg gctctccttc gaaagcctgc ctggcgagca gttgatcgta ggaggcactg
    26221 gcggctcggt cgaggaagag acccagcacg cagaaccata tgccattcag tggccggccg
    26281 tggagaatat tgaggacacc caggaagagc agaaggaatc caaaacccga tccgatgcgg
    26341 gaggccaggt gtgtcgccca tcgaagatcg cgcttccagt gccataacat cgcgcgaaac
    26401 atgcgccctc cgtccagggg gaaggccggg acaagattga acacggccag gaccaggttg
    26461 ataaatccca ggtagccgag aaccgctaaa accggaagag gaagactaag cagttgccca
    26521 agcgcgtacg ccccataaaa cactcctgct aacacgaaac tagccagtgg cccggcaatg
    26581 gccatgcgga actcggatgt ggcattcggc ggctctttgt ccatttgcgc cactccaccg
    26641 aaaataaaca aggtaatgcc ggtaatggga atatcgtatc gtcttgcgat tagggagtga
    26701 gacaactcat gcaggatgag agacccgaat agtccgatgg ccccggaaat agccagcagc
    26761 caataggtgg attccgtcaa atccggatat tcatgcggaa agaaccccat ggccagtgac
    26821 cagacaatca gcagggccag aaagggccag gtccaatcga tgccgacttt gaatcccagc
    26881 agttggaaca gtgtgactgt gcgcttgagc atacgtggga accttatcaa ccgtatgagc
    26941 gatggttcat gatgatgccc gccgaagaag tttcttgagt tcattaagac ggagggtatt
    27001 caggacatcc tctggctcca accacccccg acgggcttgc cccacaccga agcgcatgaa
    27061 atccagatcg gtcgttcggt gcgcgtcggt ggaaatgacc actttcactc ccatatcctt
    27121 cgccattttg cagtggcgat cattcaaatc gaggcgtacc ggttgggcgt tgacctccag
    27181 gcaacatccg cgctccaagg ccgcggtcat gatttgttcc atgtcgatct catacggttc
    27241 ccgttcattg atcatgcggc cggtgggatg tgccaggata gagaaagagg gatgatccat
    27301 cgcccgaatg atgcgctcgg tttgtttcgc ccgtggcaaa ttaaatttgt aatggatggc
    27361 gcacaccgtc aggtccaaac gttgtaacag gtcgtcgcag agatccaggg acccatcttc
    27421 caggatatcc acctcggccc ctttcagaat ggtgaagcct ttcaatgtgt cattgagctg
    27481 gtcgatttcc tcaatctgcc tcgctaggcg gttggcatcc aatccgtgcg caatggtgac
    27541 gtgttgggaa tggtcgcaaa tggcgaggta gttataccct ttgccttggg cggccagggc
    27601 catctcctca atagagaaat ggccgtcgct ggctttggaa tgcacatgca gatcgccacg
    27661 gatatcgttg agcgtgacga ggtgggggag ggcatgatgg agaccggctt ccacctcacc
    27721 ccaattctct cgtagctccg gctcaaggta agggagaccg acctgggcat agacttcttt
    27781 ctccgtccgt cccgcga
//
LOCUS       DATIAL010000421        10103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_506703, whole genome shotgun sequence.
ACCESSION   DATIAL010000421 DATIAL010000000
VERSION     DATIAL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506703"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VLA60_13080"
     CDS             <1..525
                     /locus_tag="VLA60_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HSF10341.1"
                     /translation="RAGDVVAILGIGGLGHLGVQYANKMGFETVAIARGKDKEALAKK
                     LGARHYIDSRAQNVAEALKGLGGAKIILATVTSGKAMSATIGGLAIDGKLIMVGSSEE
                     PVEVPIVQFIMGRHSVQGWPSGTSSDSQDTLAFSVMTGIRPMIEEYPLERAAEAYERM
                     MSGEARFRVVLKVS"
     gene            complement(910..1152)
                     /locus_tag="VLA60_13085"
     CDS             complement(910..1152)
                     /locus_tag="VLA60_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HSF10342.1"
                     /translation="MIPADVKLDTLGYFCPMPIILTSKKIKELASGQVLEVISDDEGI
                     KKDMPAWCETTGHAMVGMEEEGAADKKVYKAYVKKA"
     gene            complement(1176..1376)
                     /locus_tag="VLA60_13090"
     CDS             complement(1176..1376)
                     /locus_tag="VLA60_13090"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSF10343.1"
                     /translation="MKDNRMPGIPELMETAKDLDVKMIACTTTLGLMGISKDTLIDGI
                     DQLAGVTTYLAEARHGSVNLFI"
     gene            complement(1376..1705)
                     /locus_tag="VLA60_13095"
     CDS             complement(1376..1705)
                     /locus_tag="VLA60_13095"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HSF10344.1"
                     /translation="MNATHVEPATALAELKETKPDKVTLVVLSGDMDRVMAALIIATG
                     AAAMGMQVTMFFTFWGLNAIRKENVSSSPKDWLRRAFGWLNKGGASRLPLSRFHFGGL
                     GTTMMKR"
     gene            1970..2887
                     /gene="ftcD"
                     /locus_tag="VLA60_13100"
     CDS             1970..2887
                     /gene="ftcD"
                     /locus_tag="VLA60_13100"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HSF10345.1"
                     /translation="MDRLVECVPNVSEGLDEAVLALLTERIESVPNVALLDLHMDPDH
                     HRSVFTLAGEPERMATALFQFIQEAQHSIDLRRHQGQHPRIGAVDVVPWIPLRGVTME
                     ECADYAKDLGRRVGQELGIPVFLYEQAALVPLRARLDSIRRGGLSGLQERMYQEADWK
                     PDFGPNVLHPTAGAVAIGARFFLIAFNVVLKSQDIQVARRIARTIRSSGGGLPALKAM
                     GVPLSSKGLVQVSMNLMDFRQTSLRAAFQAVERESHRLGVEIQESEIVGLVPQEAWDA
                     DLATDLKLKNWNPEGVLEVACGKYHLFPS"
     gene            3021..3449
                     /locus_tag="VLA60_13105"
     CDS             3021..3449
                     /locus_tag="VLA60_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10346.1"
                     /translation="MSDDVAALRASKFGQLTVGSVMEKEVQSGMKDSEAKLLASYMME
                     GFGSVPIIDETSKLVGIVSEFDLLKALRKGKNLEDVTAGDIMTANPVSVTQDTNVLTL
                     IDVLQNNHLIRVPVVDSKGKLIGIVARRDLLRGYLQTSGS"
     gene            3585..4424
                     /locus_tag="VLA60_13110"
     CDS             3585..4424
                     /locus_tag="VLA60_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HSF10347.1"
                     /translation="MKDQANQIVRAPWFEYGIIAFILINGVILGLETSPALVEHYGAL
                     MHWGNHVILGIFILEALIKMIAVAPQIDRYFRDGWNIFDFSVIVFSLIPATGEFAMIA
                     RLARLLRVVRLISTIPELRLIVSTLVRSIPSMIHVMTLMGVIFYVYAIMGYQLFHEHD
                     PTHWRSLGISLLTLFRVVTLEDWTDVMYAAMEFHYLSWIYFVSFVVLGTFVVINLFIA
                     VVINNLDEAKAERLTELQGPVTQKEILKDLRETQIALKRLEERLERTSGENVLPLSKV
                     FKG"
     gene            complement(4593..5261)
                     /locus_tag="VLA60_13115"
     CDS             complement(4593..5261)
                     /locus_tag="VLA60_13115"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HSF10348.1"
                     /translation="MNDTNASLLRNARPRLTRQLISGMCGMVVCLGFLSLSPVQAAEE
                     LVGNAVNAVGDFTVFRTDGIQERLEGKGSLALYEGDRVKTGSGSQALMEFTNGIQVAL
                     NEDTDFLILSRWEDGKGFTRILRLANGEVWVKTMGGPKPLEIETPVATAAVKETEFDI
                     KVLPDGQTTLTVVEGIVEFGTAFGTCPIKTSTISYGNRGKKCTKPAPVDVAPNVAWAQ
                     GIRQ"
     gene            complement(5340..6347)
                     /locus_tag="VLA60_13120"
     CDS             complement(5340..6347)
                     /locus_tag="VLA60_13120"
                     /inference="COORDINATES: protein motif:HMM:NF028129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5074 domain-containing protein"
                     /protein_id="HSF10349.1"
                     /translation="MMPRSRSTISPSAGLVLFLIALLTGCSAFAPPPKPEGLNTRVYT
                     ANESSNTVTVLDGDTYQVIGDIETLNYGAHDLALSRDGKQLWVTNLANGRVSVIDTES
                     RKTIASIYTGTRAHVVALTNNNKHAWVANISDNTISIIDTTIFRILGTIPVGKGPMGI
                     AFSRDGKFAYVSTQDKMVNIIDTSAHQVIKQIPVGPNPHFLTLGPDGNIWGTNTGSND
                     IYVIDTTTNEIAKTIEVGPAPQQIAFALKGTAGPNAYVTVTGINKVVMVDATSPTLAI
                     REQIDVGDGPNGIWANAVGTKIYVGHTQSNNVMVVDTGTSQVLSTLPVGRKPIRVVAS
                     R"
     gene            complement(6389..9934)
                     /locus_tag="VLA60_13125"
     CDS             complement(6389..9934)
                     /locus_tag="VLA60_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSF10350.1"
                     /translation="MIIVPPTLRQLSPALLWITLLVFCGVVGEGWLPFSYGAPPPPRT
                     LSGHQGEIFGLAFDPTGQIIASGSGDQSIRLWDVETGRLQTVLKGHTAPVRTLAFSPK
                     GNFLASGASDKTTRIWDVQTGKETASFSSVFGNIRAVAFSPDGEVLASTGDGGSLRLL
                     DWKAKKELKAMKSGFGIIYAVAFSPSGSTLATGSSDTHVHIWDPSSGQQQTVFSGHTK
                     AVLSVAFSPDGSLLASGSADGTIRLWEVQTGRERGVLLGHSGDVNAVGFMANGIHVIS
                     AGSDGTVRFWDSSTSRSLGILSNFKGPVWALALAHHEALLAIGGRDRTVHLQAATITP
                     AQTSSGASSVIPRDADAGPPPIPPPHAEADFLIQPHKLKPGDELTIDLRVSNTGKGPL
                     YRFLAKTSSSHPLLDGRTFYFGKILAGQSKSEILKVNIPKDMTTDEIPLDIQFEEYNG
                     FVPDPLKALITLSGAPRPRLAYNYQILDNGTGQSVGNGDGRIQKGEAVDLLLTIKNVG
                     AVPAKDTWVELTNQEGQHLDIRPHVIRFGQLEPNDTKQARISFTVWPDYPKQELGFGL
                     LIKEKTQNVFLNEKVDLIVDILPPQQIIATNKIVMVTQDHASLLSGAGAESSVLATVE
                     KAQTLSATGELGGWYRIQLSEKETGWISKQQVTVQSSIKGEMPIPTIQGLEANKAAQF
                     INLPEQLQQAEQERARIEQTLKQREQEMTLLQIKLEQMTSTQQSTLSTTQEELERERA
                     EREQIELALQHREREAQQLRAQLEDSANLKSSELSTMREHLQQEQAQREQAEKALRNF
                     KQEMAHLEAKLDKMNQSQVSAKTPPAIALATPFDQQVLTVDRVHLVGAAASDRGIARI
                     EVRVNDELLVRRQGRGVAVVSSQGMQQSTQEFSETIQLREGENIITVIAYDNDQLESY
                     RTLRVTREINRGQIWAVVIGISRYKTVRSLKYADKDASAFRDYLTKNVGVLPDHLTFL
                     INEEATLFNLKRSLGTELKRKAGPKDTVIIYYAGHGAPEPDASSPDGDGLEKYLIPYD
                     ADPQDLYTTGLPMREVETIFHRLSAERVIFITDSCYSGATAGRTFSTAARRATLSDGF
                     LTRLSKGKGRVVLTASRAGEVSEERDELGHGVFTYYLLEGLNGKADVDGDSIVTVDEA
                     YSYLSTHVPQATGQNQHPVKKGEVEGQLILGRIQF"
BASE COUNT         2279 a         2410 c         2756 g         2658 t
ORIGIN      
        1 cgtgccggtg atgtggttgc tattctgggc atcggcggcc ttggacattt aggggtacag
       61 tatgccaaca agatgggatt cgagacggtc gccattgcca gaggaaaaga caaagaggct
      121 ctggccaaga agttgggcgc gcggcactat atcgacagca gggcccaaaa tgtagcggaa
      181 gcgcttaagg gtcttggcgg tgcgaaaatt atcctcgcaa ccgtcacgag tggaaaggcc
      241 atgagtgcaa ctatcggggg actggccatc gacggtaaat taattatggt tggctcctct
      301 gaagaaccgg tggaagtacc catcgttcag tttattatgg gtcgacattc agttcagggc
      361 tggccgtccg ggacgtcgtc ggattcgcag gatacgttgg ccttcagtgt gatgacgggt
      421 ataaggccga tgatagagga gtatcctctt gaacgcgcag ccgaagccta tgagcgtatg
      481 atgagtggtg aagcaaggtt cagggtagtt ttaaaggtta gttgaagagt ccgctttctg
      541 ggcactcatc caatgctttg ccttcgtcaa aaacgtgaat gcccatactg aagaagctgt
      601 gctcatgaaa agagccaagg tggtcagtgg gtaagggcga caaatttctg cagcgacggc
      661 tcgactttca atactctgaa catgacatca ctcaactgac gcctgtcatc ggggattcat
      721 tcatatttcc tcgccgcagg aaatgagcgg acgagggaag gcgggggtca ttccattaaa
      781 aatgaagatc tgaatggggt attcgttgtg aaaatttcga tgttgacgta tgttacccca
      841 gcgtctaatt gctcggtaat tgatcggtta tgagaccctg gaaaagagat attctacaag
      901 agcagaatct caagcctttt tgacataggc tttatacact tttttgtcgg cggcaccttc
      961 ttcttccatg cccaccatgg cgtgtccggt tgtctcgcac caggccggca tatctttttt
     1021 aatcccttcg tcatccgaaa tcacttcaag gacttgccct gaggcgagtt ctttgatttt
     1081 ttttgaggtg agaatgatgg gcatcgggca aaaatatccc aatgtgtcga gtttaacgtc
     1141 ggctggtatc atggtagatc cttttgtggt ggtcgttaga taaataagtt tacggatccg
     1201 tgccgggctt ccgccaaata ggtggtgact ccggcgagtt ggtcgattcc atcaatgagc
     1261 gtatcctttg aaatgcccat gagtcccagg gtcgtggtgc aggcaatcat cttcacgtcc
     1321 aggtctttgg cggtttccat gagttcgggt atgccgggca tgcgattatc tttcatcacc
     1381 ttttcatcat ggtggttccg agacctccaa aatgaaatcg tgagaggggc aggcgactgg
     1441 ctcccccttt gttcagccat ccaaaggctc gacggagcca atccttaggg gagctactca
     1501 cattttcctt ccggatggca ttcaaccccc aaaaggtaaa aaacatcgtc acctgcatgc
     1561 ccatggctgc agcgcccgtg gcgataatca gggccgccat gacgcggtcc atgtctccac
     1621 tcaggacgac aagtgtcact ttatcgggtt tggtttcttt gagctcggct aaagcggttg
     1681 ctggttcaac gtgggtggcg ttcatgatgt caccttcttt ctcagaaaaa tgatgggtcg
     1741 cctcctaaag agtcctcgat ataggcggat tgttacggag tataacagat cctgcccttt
     1801 gagagcgttg attttgcttg gaggcattat ccaaggatga ggcttaccgc tcattcgact
     1861 ataatatggt aattttttcg gtgtcaacaa aagcctgttc tggattgtgc ctgcatgagg
     1921 cctgaaccgc aggcttctta gccgtgtagg cagatgtaag aaagtggcca tggatcggtt
     1981 ggtagaatgt gtgccgaatg tgagtgaggg cctggatgag gccgtactcg ctttattgac
     2041 tgagcgtatt gagtcggtac ccaatgtggc cttactcgat cttcatatgg atcctgatca
     2101 tcatcggtcc gtttttaccc ttgcagggga gcctgagagg atggccactg cgctttttca
     2161 gtttattcaa gaagcacagc atagcatcga cctccgccga catcaggggc agcatccacg
     2221 catcggggcg gttgatgtgg tgccctggat tcccttacga ggcgtgacga tggaggagtg
     2281 tgcggactat gccaaagatt tgggcaggcg agtcggacaa gaactgggca ttcccgtctt
     2341 tctctatgaa caggcggctt tagtgccctt gcgggcccgg ctggatagta tacgccgggg
     2401 ggggctttct gggttacagg aacggatgta tcaagaggca gattggaagc ctgattttgg
     2461 cccaaacgta ttgcatccta cggcaggagc ggtcgcaatc ggagcccgtt tcttcctgat
     2521 tgcctttaac gtggtcctca aaagtcagga tattcaagtg gctcgtcgta ttgcccgcac
     2581 gatccggtct tcaggtggag gcttacctgc gctcaaggcc atgggtgtgc ccttgtcatc
     2641 aaaagggctt gtgcaggtgt ccatgaatct gatggatttc cggcaaacgt ccttgcgagc
     2701 ggcatttcag gctgtggagc gggagtccca tcggttgggt gtggaaattc aggaaagtga
     2761 aattgtgggc ctggttccgc aggaggcatg ggatgcggat ttggctaccg atcttaagtt
     2821 gaagaactgg aaccctgaag gtgtgcttga agtggcgtgt ggaaaatacc atcttttccc
     2881 ttcgtaactc atcaggtaca atgaccgcga aatgtgaaga tgaattccac gtcctactga
     2941 attgtgagcc ggggcactgg aatcattcga aagagcaacg acgtgggcat gaacttttac
     3001 tcaaaaaagg aaaaaaccat atgagcgatg atgtggcagc tttgcgggcc agtaaatttg
     3061 ggcagttaac ggtgggcagt gtcatggaga aagaagtgca atcgggcatg aaggattcgg
     3121 aagccaagtt actggcctca tatatgatgg aaggatttgg ctcggttccc atcattgacg
     3181 aaacctcgaa attggtggga atcgtttccg agtttgattt actgaaggcc ttgcgcaaag
     3241 gaaaaaacct ggaagatgtg acggcaggtg acatcatgac tgccaatccg gtatcggtta
     3301 cgcaagatac caatgtgctg acgctgatcg atgtcctgca aaataatcat ctgattcggg
     3361 tgcctgttgt ggattcgaag ggaaaattga tcgggatcgt tgcgcgtaga gatttactac
     3421 ggggctattt gcaaacatcg gggagttgaa gctccccgag gaattgtgct acggctttcc
     3481 ttcactgggt tcatccatcc cattttccaa cattaatgaa tcggcgctcg ttctcttcct
     3541 ggttccaggg tgggaatgcg ccatgagtct gtgagttgac gacgatgaaa gatcaagcga
     3601 atcagattgt ccgcgcccca tggtttgagt atgggatcat tgcctttatt ctcattaacg
     3661 gagtcattct gggcctggaa acctcaccgg cattggtgga acattatggt gccctgatgc
     3721 attggggaaa tcacgtcatc cttggaattt ttattctgga agccctcatc aaaatgattg
     3781 ccgtggcacc gcagattgat cggtattttc gagatggctg gaacatcttt gatttttccg
     3841 tcatcgtgtt ttctttgatt ccggctaccg gtgaatttgc catgattgcc cggttagccc
     3901 gcttgttgcg ggtggttcgt ctcatctcga ccattcctga gctccgcctt attgtttcaa
     3961 ccctcgtcag atctattccc agcatgattc acgtgatgac tctcatgggc gtgatctttt
     4021 atgtctatgc cattatgggc tatcagttgt ttcatgagca cgatccgacg cattggcgat
     4081 cattggggat ctcgctgttg accctgtttc gcgtcgtgac cctggaggat tggactgatg
     4141 tgatgtatgc cgccatggaa tttcattacc tgtcttggat ttattttgtg agttttgtgg
     4201 ttttagggac gtttgtcgtg attaatctgt ttattgccgt ggtgatcaat aacctcgatg
     4261 aggccaaagc cgaacgcctg actgaacttc aggggccggt cacacaaaaa gagatactca
     4321 aggatttgcg agaaacacaa atagccctca agcgcctaga ggagcgcctg gaaagaacgt
     4381 ctggagaaaa tgtcctaccc ctatcgaaag tgtttaaagg ttaaaatccc cggaaccatg
     4441 ttggttacgt tccaagggca atcgtggaaa ggaatgatag agaaagaagg ggcggggata
     4501 atccactagg ccagatccca acacgaaaaa atcgggcccg ttccggtctg ccggaacggg
     4561 cccaatttat ggcgataacg taacggtaat tgctactgac gaataccttg ggcccacgcg
     4621 acattagggg ctacgtcgac cggagccggt ttcgtgcatt ttttcccgcg attcccatag
     4681 ctgattgtgg aagttttgat cgggcaggtt ccaaacgcag tgccaaattc cacgatcccc
     4741 tccaccacag tgagagtcgt ttgaccgtcc ggtaagacct tgatgtcaaa ttccgtttcc
     4801 ttcacggctg cagtggctac aggagtttcg atttccaatg gttttggtcc tcccatggtt
     4861 ttgacccaaa cctctccgtt cgccagacgt aagatcctgg tgaatccttt accgtcttcc
     4921 cagcgagaga gaataaggaa atcggtgtct tcgttaaggg ccacttggat cccattggtg
     4981 aattccatta atgcctggct tcctgagccg gttttgactc ggtctccttc gtataacgcg
     5041 agggaccctt tgccctcaag tcgttcctga ataccatcgg ttcgaaatac cgtgaaatct
     5101 ccaacggcat ttacggcatt ccccaccagt tcctcggcgg cctgaaccgg agagagggaa
     5161 agaaatccca gacagacaac catcccacac attccggaaa tgagttggcg ggtcagacgt
     5221 ggtcgtgcgt tgcgtaaaag ggatgcattg gtatcgttca tgtgagcctc ctattcagca
     5281 tttgaaaaat catcaacttg aatgatcatg gagggatgat cccttcccca tgatgtggtt
     5341 caccgggatg cgacgacccg aatgggtttt ctcccgacgg gaagggttga taggacctgg
     5401 cttgttccgg tatcaacgac cattacattg tttgattgtg tgtgaccgac atagattttg
     5461 gtgccgacgg cattggccca gattccgttg ggtccatctc ccacatcaat ttgttcgcga
     5521 atggcaaggg ttggggaggt ggcatcaacc atgacgactt tattaatgcc ggtgaccgtg
     5581 acataggcat tggggccggc agttcccttt aaggcaaacg cgatttgttg cggagccggg
     5641 ccgacctcga tcgttttggc gatttcattg gttgtcgtgt cgatgacata aatgtcatta
     5701 ctgccggtat tggttcccca tatgttgcca tcgggcccca gcgtgagaaa gtggggattt
     5761 ggtccgaccg ggatttgctt gatcacttgg tgtgcggatg tgtcaataat gttgaccatt
     5821 ttgtcctggg tactgacata cgcaaatttc ccgtcacggg aaaaagcaat gcccatcggg
     5881 ccttttccga ctggaatcgt cccgagaatg cgaaagatcg tggtatcaat gatcgaaata
     5941 gtgttatcgg aaatattggc cacccatgca tgtttattgt tgttggtgag agccacgaca
     6001 tgggcgcgag tgccggtata aatcgatgca atcgttttac gggattcggt atcaatgacc
     6061 gagacccgtc cattagccag attcgtcacc cagagctgtt tcccgtcacg gctgagagcc
     6121 aaatcgtgcg ccccataatt cagcgtttcg atatcaccga taacttgata cgtgtctcca
     6181 tcaaggaccg tgacggtatt ggagctttca tttgccgtat agacccgggt atttaatcct
     6241 tcaggtttgg ggggaggtgc aaacgcagaa caacccgtca ggagagcgat caggaaaagc
     6301 accagaccgg ctgaggggga aattgtcgaa cgcgaacgtg gcatcatgag ggggtcctcc
     6361 tgcattgggg tggaaataat cgtatctttt aaaattggat ccgtccgaga attaactgtc
     6421 cttccacctc accctttttg acggggtgtt ggttttggcc cgtggcctgg gggacgtggg
     6481 tggaaagata ggaatatgct tcatcaaccg tcacgatgct atctccatcc acatcggctt
     6541 ttccattcag cccttcgagc aaatagtagg taaagacgcc atggcccagc tcatcgcgtt
     6601 cttcactgac ttctccagcc cggctggctg tcagcacgac ccgtcctttc cctttggaca
     6661 gtcgggttaa aaatccatct gaaagggttg ccctgcgggc ggcggtggaa aatgtccgtc
     6721 ccgcagtggc gccactataa caagaatctg taatgaaaat gactcgttcg gcggacaatc
     6781 gatggaaaat tgtttcgact tcccgcatcg gcaggccggt ggtatacaaa tcctggggat
     6841 cagcatcata gggaatcagg tacttttcca ggccatcccc gtccgggctg gaagcatcag
     6901 gttcaggggc tccatgaccg gcataataga taatgacggt gtccttcggg ccggcttttc
     6961 gttttaattc cgttcctaac gaccgtttca gattaaacag tgtggcttcc tcattgatta
     7021 agaacgtcag gtgatcgggc aagactccca cattcttggt gaggtaatcc cgaaacgctg
     7081 acgcgtcttt gtcggcgtac tttaacgatc tgacggtttt gtatcgggaa atgccgataa
     7141 cgacagccca gatttgtccc ctgtttattt cccgggtgac ccgaagggtt cgataggatt
     7201 ccaattggtc attatcatag gctatgacgg tgataatatt ttctccctcg cgaagctgaa
     7261 tcgtttcaga aaattcctgt gtcgattgtt gcatgccttg cgaggagacg acggccactc
     7321 cgcgaccctg gcgtctgacc aacaactcat cattcacgcg aacttcgatt cgggctatgc
     7381 cacggtcgct ggccgctgct cccaccaggt gtacccggtc gactgtcaga acttgttgat
     7441 caaacggggt tgctaaggca atggctggag gcgttttggc ggaaacctgg gactgattca
     7501 ttttgtccaa tttggcctcc aaatgggcca tttcctgttt gaaatttcgc aaggccttct
     7561 ccgcttgttc ccgctgtgcc tgttcctgct ggagatgttc gcgcatggta gatagctcgg
     7621 aagatttcaa attggctgaa tcttcgagtt gggcccggag ctgctgcgct tcccgctcac
     7681 gatgttgtaa cgctagttcg atctgttccc gttcggcccg ctcccgctcc aattcttcct
     7741 gggtggtgga aagggtggac tgttgcgtac tggtcatttg ctccagtttg atctgaagaa
     7801 gagtcatttc ctgttcacgc tgtttcaagg tctgttcgat gcgagcccgt tcttgttccg
     7861 cttgctgaag ctgctcgggc aggttgatga attgagccgc cttattggcc tccaggccct
     7921 gaatggtggg tatgggcatt tcgcctttta ttgaactctg gacggtgact tgttgcttgg
     7981 aaatccagcc tgtctctttt tctgacagtt ggatccgata ccagccgcca agctctccgg
     8041 tggctgagag ggtttgcgct ttttcgacgg tggccagcac ggaactttcc gcccctgctc
     8101 cgctgagcaa ggaggcatgg tcctgcgtta ccatcacgat tttattcgtg gcgatgatct
     8161 gttgaggggg caggatatcc acgattagat ccactttttc attcaaaaac acattttgag
     8221 ttttttcctt gatcaataac ccgaagccga gttcctgctt ggggtaatcg ggccacacgg
     8281 taaaactaat gcgggcctgt ttggtgtcat tcggctccaa ttgaccgaat cgaatgacat
     8341 gaggccggat gtccaaatgc tgcccttcct gattggtcaa ttccacccag gtatcctttg
     8401 cgggcaccgc gcccacattt ttgatggtca acaacaggtc aaccgcttca cctttttgaa
     8461 ttcggccgtc accattgccc actgactgcc cggtgccgtt atcaagaatt tggtaattat
     8521 aggctaagcg gggccgcgga gctccggaga gggtgatgag ggccttgagg gggtccggca
     8581 caaatccgtt atattcttcg aactggatgt ctaacggaat ttcatcagtg gtcatgtctt
     8641 tgggaatatt tactttcaat atttccgatt tcgattgccc ggccagaatt tttccaaaat
     8701 aaaaagtccg accgtccagc aatgggtgtg agctactggt tttggccagg aaccggtaca
     8761 gcgggccttt tccggtattg gaaacacgga ggtctatcgt gagttcatca ccaggcttta
     8821 gtttatgagg ttgaatcagg aaatccgctt ccgcatgtgg gggagggata gggggtggcc
     8881 cggcatcggc atctctgggg atcactgatg aagctccgga cgacgtttgt gccggagtga
     8941 tagtggccgc ttgaagatgg acggtccggt cgcggccccc aatggccagg agcgcttcgt
     9001 gatgggccag agccaaggcc cataccggcc ctttgaaatt ggaaagaatg cccaaggacc
     9061 gactggttga gctatcccag aaacggacgg ttccatcgct tccggcagaa attacgtgta
     9121 tcccattggc cataaatcca acggcgttca cgtccccgga atggccgaga aggacccctc
     9181 gttcccgccc agtttggact tcccagagtc tgattgtgcc atcggccgat ccactagcca
     9241 gcaaggatcc atccggagaa aatgcgacgg agagaacagc tttggtatgt ccggaaaaga
     9301 ccgtttgttg ttgaccgctt gagggatccc agatgtgcac atgagtatcg ctgctaccgg
     9361 ttgctaaggt tgaacccgaa ggtgaaaaag ctacggcata gataatgccg aatccgcttt
     9421 tcatggcttt caattccttt ttggctttcc agtccaataa acgaaggctg cctccatcgc
     9481 ctgtactggc cagaacttct ccatccggag aaaaggctac cgctcgaata tttccaaaaa
     9541 cggaactgaa agaggccgtt tcttttcctg tctgcacatc ccagatcctg gtggttttat
     9601 cgctcgcgcc tgaagcgaga aaattccctt tcggggagaa ggctaacgtc cggacgggag
     9661 ccgtatgccc tttcaagacg gtttgaagac ggccggtttc cacgtcccac aatctaatgc
     9721 tttgatcgcc gcttcctgag gcgatgattt gaccggtggg atcaaaggcc agtccaaata
     9781 tttcaccttg atgtccactg agagtgcggg ggggcggagg ggctccatag gaaaaaggca
     9841 gccatccttc tccaacgacg ccacaaaata cgaggagcgt gatccacaat aatgctggtg
     9901 aaagctggcg aagagttggg ggtacgataa tcatgatcgc atgtttaaaa aaaagcctgg
     9961 ttcacctttg ttgctcagtc tgtcggattt caggactcaa gggttcatct gattacactc
    10021 ttttcctggg gaaaaacgat gaggggcccg cctgtctgcc tgttcattca tggaagaacc
    10081 gaatgattga gaagccctat tga
//
LOCUS       DATIAL010000422        10491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1366980, whole genome shotgun sequence.
ACCESSION   DATIAL010000422 DATIAL010000000
VERSION     DATIAL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366980"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VLA60_13130"
     CDS             complement(<1..1119)
                     /locus_tag="VLA60_13130"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF10351.1"
                     /translation="MAATFPVLSWVHGLHFNNIRGDLYGGLVAAVVALPLALAFGVTS
                     GLGAIAGLYGAIFVGFFAALFGGTPAQASGPTGPMTVVMAGIILNFADEPALAFTAVI
                     LGGVFQVLFGLMGFGRYITLVPYPVISGFMSGIGGIILILQVAPFLGLEAKSKVVDNL
                     MAMPEYFASLSLPAMLVGSLTLAIVYLTPASIGKRLPPVLLALIVGTMVAEMFFPTLP
                     VIGDIPTGFPTPIWPTMHTNTLLMMVEEALVLALLGTIDSLLTSLVCDNMTRTQHDSN
                     RELIGQGIGNMAAGFFGGLPGAGATMRSVANIRTGGRTPLSGMFMAVVLLGTLLGLGP
                     LAEKIPLAVLAGLLIKVGFDIIDWRFLKHMVQAPRTEVL"
     gene            complement(1243..2964)
                     /locus_tag="VLA60_13135"
     CDS             complement(1243..2964)
                     /locus_tag="VLA60_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSF10352.1"
                     /translation="MPSPLSLQNSVVRPQKNRGQVLALALLVATVVFVVDLFLPMGVA
                     EAVPYVASVLIALRSPDKNDPLRLAGLCTLLTIIGFYFSPSGAEPWIGMTNRSIALFA
                     IWSTALLAMQVRESDEATRDQSSMLTGILSNMPAVAFRLDQEGVMHDSFGRGLARFGL
                     KELTAIGRIPLEPPDQIKNQVKEGTLANSVFYESKGILQGKPWWFLNCLCKLEGDQPG
                     LIGFGIDITDRKRAERRLALHDVIADILTTASSISETYPQILKAICHTLEWKVGILWR
                     MDYQRQALGATLIWPTDSPQAQAMSSAHSGWALTPAVGLAGQVWATRNPLWIKDVAEY
                     PKDPRSQIALEVGLHAGFAIPICLAENTRAVLEFFSPYPEEADDMLLQRLSNIGFQIG
                     QYIEREHKERRLATHHSLTNVLAESAGMTQAASLILEAIGDNLGWDLGAIWTLDSSQQ
                     VLRCLNVWHSPHLKLDAFRQESQSITFARGVGLPGRVWKSGEPIWITDVVSDPNFPRA
                     AAAAQEGLHSGFAFPVKSGAEFVGVIEFFHRDIQKPDKELLVMFASIGLQIGQFIQRT
                     AKQSLNG"
     gene            3253..3621
                     /locus_tag="VLA60_13140"
     CDS             3253..3621
                     /locus_tag="VLA60_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10353.1"
                     /translation="MSSTSPHVHIFHVAGLDDRDRGFNRQIHVIPREGGFQARLRYES
                     LQIDVDPVPTEDQVLQGLIHMLHERGYRQLRTQRIFVGEQYLGNQELWVEYPDPEAPL
                     ETRKTWVAWIRQIVGRSFRS"
     gene            complement(3691..5076)
                     /locus_tag="VLA60_13145"
     CDS             complement(3691..5076)
                     /locus_tag="VLA60_13145"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSF10354.1"
                     /translation="MPFVPLHATALRLGLYIKIEGSWFSHPFPTNSFKIESAKDLETL
                     RGLTKVKLYFDPNRSEPEGIHSDHTKAPDRKGVQSTVEQNKEPSNESKSITPQHSSEE
                     EATPEDPIQRKVAQHQAFQVYQEHLQAVGGQFQEVVQEAKQVMQDVITGRPRGLRTAQ
                     KIVSELYEILDIADNSRALLNLIGSNEPNEEFFLHALNVCSLSLMVGQDLGLSREDTE
                     KLALGALFHDIGELKFPAEQLLRKGSMSASERKNFLSTHPKYGGELAEKLPNFPYEAI
                     DVIRQHHERLNGSGFPTGKKDDQISQFAKIVMVVDEYDELCHHPDPAKSLIPSEALSY
                     LYVKCRHTLWHDAVVSLISQLGVYPPGSLVNLSNQKIGIVTSVNLANRLRPVILVYDE
                     YTSPDEPIILNLSEEDESLSIVSAIRPVDLSAKIRECLNPRRIISYFPSASASSSESG
                     VGGLRTLAGST"
     gene            complement(5579..6682)
                     /locus_tag="VLA60_13150"
     CDS             complement(5579..6682)
                     /locus_tag="VLA60_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSF10355.1"
                     /translation="MISQQQCQNATILIVDDQMTNIMFLESILKNGGYTQVHSTQDPT
                     QVIPLFKELDPDLICLDIRMPELNGFQVMGQLKIIHKHGFLPILVLTSEEDRETRLRA
                     LESGAKDFLPKPFDKVEVLMRIRNLLEASLLNKTVSQQNDILEETVRIRTQELKDTQL
                     EVVHRLARAAEHRDNETGAHIIRMAHFAVVLGRAAGMSEDECDVLFHATPMHDVGKLG
                     IPDRILLKPGKLDPEEFEIMKQHTVIGAQLLSNSHSPVLQMGEVIALTHHEKWDGSGY
                     PNRIAGEDIPIVGRICAIADVFDALSSKRCYKDPWPLDKTLKEMHSLSGKQFDPHLLD
                     LFNELLPVMTDIQQTHTDKEIPKFDLLKTNNHV"
     gene            complement(6976..10071)
                     /locus_tag="VLA60_13155"
     CDS             complement(6976..10071)
                     /locus_tag="VLA60_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10356.1"
                     /translation="MGNALNHLGVQAVKQMENTLENPVESILNPDPFQSYVHALSERL
                     DQYPTGDTHDILSQMLKTGQRLQALGVEVRKWVQHSGPILKDLQSQQTIKMARERLKN
                     LRNQIQTWEEKTRLAQTMAYRKGKQASPSEALEIADDLLAHQIRHAALGVSIIQTELG
                     NLSVLLETLYGERNPDRLVDLKDSQLQQNLTRLALGMNMLGGNSPIPNDTVQKILQDL
                     GSIFFGQGYSIDKTHQTIGLGSGGLYRFKQNSLRLEAERAEFYVRLEDIKEQIGEAQV
                     SFAKLASQQTQEVTNKFENSFAQSWRFMLTLSLICVAGFLSTAWIISRWIHKQLTLLE
                     TSKTQAEAAVKAKSQFLATMSHEIRTPMNGVIGMTGLLLETDLTSQQRQLANTVRSSG
                     ETLLTLINDILDFSKIEAGKLEFEIIDFDLRTTLEDTLDLLAGPAGTKQLELVGLVSA
                     TVPTALQGDPGRLRQILMNLVGNAIKFTARGEVVVQMEVLDDTPDTVTIRVEVRDTGI
                     GIAPEAHAKIFQPFSQADSSTTRQFGGSGLGLAISKQLVEQMGGTLGVESRPGQGSTF
                     WFTACLTKQPAPAQSRTPANTHLVGLTVCGVDDHPTNRQLLAQYFQDLCMTGSLAATP
                     TDALTLLRQAAHQGRPVDLAIIDMEMPDMDGLQLARTIKADPNTAAVRLVLLTSLGTR
                     GDAKAAQEAGFVGYLTKPIRKDQLQVCLETVMGYEPMDPTAVSRPLITSHRLQKWCRP
                     KAARILVADDHHVNQQLAVMLVESLGHRADVVANGQEALEALSRIAYDAILMDCHMPE
                     MDGYEATRAIRAQEAKEGKHNELGDKKPVHSQPFLLTSDTILPIPKHMPIIALTANAM
                     QGDRVKCLQAGMDDYLSKPIRPEELIRVLTKWLPHQIFDIPTQDEGPYPLPHSSPSGS
                     TGQPAVNTQTLKELEELGGRAFLESMIQKFVEDALQCVTLIEHALDKHNLTQLNEAAH
                     GLKGISRNMGADSLAQLAVQIEATCKAGEVAALSNWEPQLHDVFQQTRTELENVFNRA
                     K"
BASE COUNT         2497 a         2517 c         2916 g         2561 t
ORIGIN      
        1 cagaacctcg gttcgtggag cttgcaccat gtgtttcaga aatcgccaat caataatatc
       61 gaatccaacc ttgatgagca gaccggcgag aaccgcaagt ggaatttttt cagccagagg
      121 tcctaagcca agcagggtgc ccaagaggac aaccgccata aacatgccag aaagaggcgt
      181 gcggcctccg gttcgaatgt ttgccacgga tcgcatagtg gccccggctc caggaaggcc
      241 tccgaaaaac cctgcggcca tattgccaat gccctggccg atcaattccc ggttggagtc
      301 atgttgggtt cgtgtcatgt tgtcacagac gagagaagtc agcaggctgt caatcgttcc
      361 caataatgcc aggacaaggg cttcttcaac catcatgagg agtgtattgg tatgcatggt
      421 tggccaaatt ggcgtgggaa atcctgtagg aatgtctccg ataaccggaa gggttggaaa
      481 gaacatctcc gcgaccattg ttccaacgat gagagccaac aacaccgggg gaaggcgttt
      541 gccgatagag gcgggagtga gatagacaat ggccaatgtg agagacccca ccaacatggc
      601 ggggagagaa agcgaagcga aatactcagg catggccatg aggttgtcca cgactttaga
      661 cttcgcttcc aagccgagaa agggggcaac ctgaagaata agaataattc ctccgatgcc
      721 gctcatgaat cctgaaatga ccggataggg aaccaaggtg atataccgac caaaccccat
      781 cagtccaaat aagacttgga agaccccgcc gaggatgaca gcggtaaatg ccaatgccgg
      841 ttcatcggca aagttcaaaa tgatgccggc catcaccacg gtcatcggac cggtcggtcc
      901 tgaggcctgt gcaggagtcc cgccgaagag ggctgcgaaa aaccccacaa aaatagctcc
      961 atacagaccg gcaatggccc cgagtccaga tgttactccg aaggccaagg ctaacggtaa
     1021 ggccacgacc gcggccacta gaccaccata caggtctccc cgtatgttat tgaaatgaag
     1081 gccatgaacc catgaaagca ctggaaaagt agcagccatt ttttaggagg gaggcggtag
     1141 attcggatga taggtgacca atgctgccag gaaatgatac cccacgggca tgtcgttggt
     1201 taccaattaa tcaaaccgaa aattaattta caagaccatc gattatccat tgagactttg
     1261 cttggcggtc cgctggatga attggccaat ttgaagacca atggaggcaa acatgaccaa
     1321 cagttctttg tcgggctttt gaatatccct gtggaaaaat tcaatgacac caacaaattc
     1381 ggcccccgat ttcacaggga aggcgaatcc ggaatggagc ccttcttgtg cggcagcggc
     1441 cgcgcgcgga aaattcgggt cggataccac atcggtgatc cagatcggtt cgccgctttt
     1501 ccaaacccga ccgggtaatc ccacccctcg tgcaaacgtg atcgattggg attcctgccg
     1561 aaaggcatct agctttaagt gaggggaatg ccagacattg aggcaacgca agacctgttg
     1621 tgaggagtcc agtgtccaaa tagctccgag atcccatcct aaattgtcac caatggcttc
     1681 caggataagg gaagccgcct gagtcattcc agcagattcc gcgagaacat tggttaaact
     1741 gtgatgcgtg gccagtcgac gctccttatg ctcccgttcg atatactggc caatttgaaa
     1801 accgatattc gaaagccgtt gaagcaacat atcatccgct tcctccgggt atggggaaaa
     1861 aaattcgaga accgctctcg tattctcagc caggcaaatg ggaatcgcga atccggcatg
     1921 aagtcccact tccaaggcaa tctggcttct aggatctttg gggtattcgg caacgtcctt
     1981 gatccaaaga gggtttcgag tggcccacac ctgcccggcc agtcctacag caggcgtgag
     2041 cgcccaaccg gagtgtgccg atgacatggc ttgagcttga ggagagtccg ttggccaaat
     2101 gagggtggct cccagggcct gtcgttgata gtccattcgc cagaggatgc ccaccttcca
     2161 ttccagcgtg tggcaaatgg ctttgagaat ttgaggatat gtctctgaaa tgctagacgc
     2221 ggtggtcagg atgtctgcga tgacatcgtg caaggccagg cgtcgctccg cacgcttacg
     2281 atcagtaata tcaatgccaa acccaatgag gccaggttgg tcgccttcca atttgcacag
     2341 acaatttaaa aaccaccagg gcttcccctg cagaattccc ttgctctcgt aaaacaccga
     2401 attggcaaga gtgccttcct tcacctggtt ttttatctga tcgggtggtt ccaaggggat
     2461 acggccaatg gcggtcagtt cctttaagcc gaaacgagcc aggccgcgac cgaaggaatc
     2521 atgcatgacc ccttcctgat cgagtcgaaa tgccacggcc ggcatattcg ataatatgcc
     2581 ggtcagcatg gaactttggt cacgagtcgc ctcgtcagac tctctcacct gcatggccaa
     2641 cagggcggtt gaccaaatcg caaataaggc aatcgatcga tttgtcatgc caatccatgg
     2701 ttctgcaccc gacggcgaaa aataaaaccc gataattgtt aaaagggtac agagtcctgc
     2761 cagacgcaat ggatcatttt tatccggaga ccggagggca atcaacacgc tagcgacata
     2821 agggaccgct tcggcgactc ccataggaag gaagaggtcc acgacgaaga ccactgtggc
     2881 cacaagcagg gctagagcta agacctgccc cctatttttt tgagggcgta caaccgagtt
     2941 ttgaagggac agcggacttg gcatggggtc atatgggaat acatgaaaac ggggaatacg
     3001 acacaaatat tcccctcgga tttagtcaca atctttgggt accacgatac gaaatgcacg
     3061 ccaatgagtg caagtggaat cagcggaaat tgatggccta tgccgtcagt cgggagcgca
     3121 gacttttgga cgaagacagg ctgtggcctg aagaagggaa cgggcaaggg ccggaagtgc
     3181 ctccaaaggc tcgacggcct tttccctctt atgcgaagat gacaagcgga ggtatgatag
     3241 gcccttacgc gtatgagttc cacatctcct cacgtccata tctttcatgt tgccgggttg
     3301 gatgaccgtg accgtgggtt taatcggcaa atccatgtca ttccccgtga aggaggattc
     3361 caggccaggc tccgctatga atcgcttcag atagatgtcg atcccgttcc tacggaagac
     3421 caggtcctgc aaggtctcat tcacatgttg catgagaggg gctatcgtca attgcgaacc
     3481 caacgcatct ttgttggtga gcagtatctt gggaatcagg agctgtgggt ggagtatcct
     3541 gatcccgagg ctcctcttga aacgaggaag acatgggtgg cctggatccg tcagatcgtt
     3601 gggcggtcct ttcgctcgta ggtctccacg tagcataaga taacaggtga gttcgtgagg
     3661 atggtttcgc gaggcgttct tgggaaggga ttatgtggat ccggccagag tgcgcagacc
     3721 tccgacgccg gattctgagg aagaggcaga ggcagaggga aagtagctga tgattcgacg
     3781 agggttgagg cattccctga tttttgcaga aagatcaacg ggtcgaatgg cactgacaat
     3841 agagagcgac tcatcctctt cagacaggtt cagaataatc ggttcatccg gagaggtgta
     3901 ttcatcatag acgaggatga ccggcctgag ccggttcgcg agattcacgc tggtgacgat
     3961 tccaatcttc tgattactca agttgacaag tgagcctgga gggtagacgc ccaactgact
     4021 gattaacgag acgaccgcgt catgccatag tgtgtgccgg catttgacgt ataaatagga
     4081 tagggcctct gagggaatga gagatttggc tggatccggg tgatggcaca attcatcgta
     4141 ctcatccacc accatgacaa ttttggcgaa ctgactgatt tgatcgtctt ttttccctgt
     4201 tgggaatcct gacccgttga ggcgttcatg gtgctggcga atcacgtcaa tcgcttcata
     4261 agggaaatta ggcaatttct cggccaactc ccctccgtat ttcggatgtg tactcaaaaa
     4321 gtttttccgt tcggaagcag acattgaccc ttttcgtaat aattgctctg ccggaaactt
     4381 gagttcgcca atgtcatgaa acagagctcc gagagccagt ttttcagtgt cttcccgcga
     4441 taatcctaaa tcctgcccga ccatgagaga taacgaacag acattgagtg catgcagaaa
     4501 aaattcctca ttcggttcgt tggaaccgat gaggttcagc agtgctctcg agttgtcagc
     4561 gatatcgaga atctcataaa gctcgctgac aattttctga gccgtccgga gtcctcgagg
     4621 gcggccggta atcacgtcct gcatgacctg tttggcttct tgcaccacct cctggaattg
     4681 accaccaact gcttgcaaat gttcctggta gacctggaag gcttggtgtt gggcgacttt
     4741 tcgttgaata ggatcttcgg gagttgcttc ctcctctgag gaatgctgtg gggtgatgga
     4801 cttagattcg ttcgaaggtt ctttgttctg ttctaccgtg ctttgaaccc ccttccggtc
     4861 aggcgccttg gtgtggtcgg aatgaatccc ttctggttct gaacgatttg gatcgaaata
     4921 taacttgacc ttcgtgagtc cacgaagtgt ttcgaggtct ttcgctgatt cgattttgaa
     4981 tgaattggtt gggaacggat gactgaacca ggatccctca atcttgatgt acaatcccag
     5041 tcgcagagct gttgcgtgaa gtggaacgaa aggcatatta actctcgatt ccagtcataa
     5101 ggcatgatct ggcttgtctg gcaatcgggt cccatggttg tgagtgatca tggggcaaag
     5161 aatatgcaga ttatctgccc cgttgtgagc cgtttttcta taaccgtgtt gcccactgac
     5221 tttgtcggaa gggacgaatg taaaattaag gggaagtccg ggaaaagagt gacaaagaaa
     5281 tgagaatgga gtgccatgag ccacgaggga ggttgaggca tggcctttat cccgcattat
     5341 cccgaggggt cattacatcc accaatcaga caggcgggga attcgcctgg agccaaagga
     5401 tttggaaaga ggatcagatc cttttgatca ccctggtcta tttggatacg caatgaatga
     5461 aacactcttg gaacgtcaaa tgccggggag aaaatcaagc gtactaccct aaaaggagca
     5521 agtgggtgaa tgagagagga aacgggtcta cggagcgcaa atggtgggaa tgagttgctt
     5581 agacgtggtt gttggttttc agcaggtcga atttgggaat ctctttatcc gtgtgggttt
     5641 gctggatgtc ggtcattaca ggcagaagtt cattaaacaa gtctagcaga tggggatcga
     5701 actgttttcc tgaaagggag tgcatttcct tgagcgtttt atccagaggc cagggatctt
     5761 tgtagcatcg tttggatgag agcgcatcaa aaacatcggc gatggcacaa attctgccga
     5821 ctatcggaat atcttctccg gcaatgcgat tcggatagcc agagccatcc catttttcgt
     5881 gatgggtgag tgcaatcact tcccccattt gcaggacggg agagtggctg ttggagagga
     5941 gttgagcgcc gatgaccgta tgttgtttca tgatttcaaa ttcttcggga tctaattttc
     6001 cgggtttcaa gaggatccgg tccggaatcc ccaatttccc gacgtcgtgc atgggggtcg
     6061 cgtgaaagag cacatcacac tcgtcttcgg acatgccggc ggcacgtccc aagaccacgg
     6121 caaagtgagc catgcggatg atatgcgcgc cggtttcatt gtctcgatgt tcggctgcac
     6181 gagcaaggcg gtggaccact tccagttgag tgtctttgag ctcttgagta cggatccgga
     6241 cggtttcttc cagtatgtcg ttttgttggg acaccgtttt gttgagcaaa ctggcttcca
     6301 gcaaattgcg gattcgcatt aacacttcca ccttgtcgaa tggtttaggc aggaagtctt
     6361 tggcgcccga ttctaacgcg cggaggcggg tttcccggtc ctcttctgac gtaaggacca
     6421 gaatggggag aaacccgtgc ttgtgaataa ttttgagttg tcccatcacc tgaaacccat
     6481 taagttcagg catgcggata tcaaggcaaa tcagatctgg atccagctct ttaaacagag
     6541 gtatcacttg agtgggatct tgagtggaat ggacttgggt gtacccgccg ttttttagaa
     6601 tgctctccag gaacatgatg ttggtcattt gatcatccac aatgagaatc gtggcatttt
     6661 ggcattgctg ttgggaaatc attgtagtcc gtatgacatc aaaaggatca acgtattcat
     6721 cgagaatgag gcctggttga ccggccacca gcattgagat tgggcaggcg tttcatcgat
     6781 tcctatgcga tccttccctc ctggggatgg tatttatcat tgtttatgtc ggtcaaaaca
     6841 agtgaaactt gaagtaacgt gctttttgat ttggaaagaa gccgggagca gggatgaatg
     6901 gtttagagga agagccttgc ggtaaaagtg gtgaggagca caaaggcgag gattgttctc
     6961 tctcaacgct ttcccctatt tcgctctgtt aaaaacattc tcgagttcgg tccgggtttg
     7021 ttgaaagacg tcgtgaagtt ggggttccca gtttgacaga gcggccactt ctccggcctt
     7081 acaggtggct tcaatttgaa ccgctaattg tgctaatgag tcggcaccca tgtttcggga
     7141 aatccccttg aggccgtggg cagcctcatt caattgagtg aggttgtgtt tgtccagcgc
     7201 gtgttcgatg agggtgacgc actggagggc gtcttcgacg aatttctgaa tcatggactc
     7261 cagaaacgcc cggcctccaa gttcttcaag ttcctttaag gtttgggtgt tgactgcagg
     7321 ctgtcctgtt gatccggaag gagatgaatg aggcaggggg tatgggcctt cgtcctgagt
     7381 tggaatatcg aaaatctggt gaggcagcca ttttgtcaac acgcggatca gctcttcggg
     7441 acgaataggc ttgctcaggt aatcatccat tcccgcttgc aagcatttga cgcggtcccc
     7501 ctgcatggca ttggcagtga gggcaatgat gggcatgtgt ttgggaatgg gaagaatggt
     7561 atcagaagtc agaagaaagg gttgagagtg aaccggcttt ttgtcaccta actcgttatg
     7621 ttttccttct tttgcttcct gcgcacgaat cgcgcgagtg gcctcatatc catccatttc
     7681 tggcatatgg caatccatca gaatggcatc ataggcaatc cgggacaggg cttcgagggc
     7741 ttcctggcca ttcgccacga cgtcggcacg gtgcccgagg ctttccacta gcataaccgc
     7801 caattgctga ttgacgtggt gatcgtcggc gaccagaatc cgggccgcct ttggccgaca
     7861 ccatttctgc agccgatgtg atgtgatcaa cgggcgcgag acggcggtgg ggtccatggg
     7921 ctcatagccc atgactgttt ccaggcagac ctgtaactgg tcttttcgaa tgggcttggt
     7981 cagatagccg acaaacccgg cctcttgagc ggccttggca tcgccccgcg tccccaagga
     8041 ggtgagcagc acgaggcgca cggccgcggt gttcgggtcg gctttaatgg tgcgagctaa
     8101 ctgcagccca tccatatccg gcatttccat atcaatgatg gcgaggtcca cgggcctgcc
     8161 ttggtgggct gcctggcgga ggagggtcag agcatccgtg ggagtggcgg ccagagagcc
     8221 agtcatacac aagtcctgaa agtattgcgc caataactgg cgattcgtgg ggtgatcgtc
     8281 gactccgcag acggtgaggc ccaccaggtg ggtgttggca ggggtgcggg actgggcggg
     8341 ggcgggttgt ttggtcaaac acgcggtaaa ccaaaaggtg ctgccctgcc ccggtctgct
     8401 ctccaccccc agggttccgc ccatctgttc tacgagttgt ttggagatgg ccaggcccag
     8461 gccggagcca ccaaactggc gggtagtcga actatcggcc tgcgaaaagg gctgaaagat
     8521 tttggcgtgg gcctcagggg caatgccgat gccggtatcg cgaacctcca cccgaatggt
     8581 gacggtgtcg ggagtgtcat cgaggacctc catttggacg acgacctcac cgcgggcagt
     8641 aaatttgatg gcattcccca ccagattcat gagaatttga cgcaggcgcc cggggtctcc
     8701 ttgcagggcg gtcggtaccg tggcggagac cagccccacg agttctaatt gtttcgtgcc
     8761 ggctggcccg gccagcaagt cgagcgtgtc ctccagggtg gtgcgcagat cgaaatcaat
     8821 gatctcgaac tcgagcttgc cggcttcgat tttggagaaa tcgagaatgt cgttgatgag
     8881 ggttaacagg gtttcgccgg aggaccgcac ggtattggcc aactggcgtt gctgcgaggt
     8941 cagatcggtt tcgagtaaga ggcccgtcat cccgatcacc ccgttcatag gggtgcggat
     9001 ttcatgactc atggtggcta aaaattggga tttggctttc actgcggcct cggcctgggt
     9061 tttcgaggtc tctagcagcg tcaattgctt gtgaatccac cgggaaatta tccatgccgt
     9121 ggataaaaat ccggctacac atattaggct gagggtcaac atgaatctcc agctttgcgc
     9181 aaaggagttt tcgaatttgt ttgtcacttc ttgggtttgt tgggacgcca gtttggcgaa
     9241 tgacacttga gcctcaccaa tctgttcttt tatgtcttcc aagcgtacat agaattccgc
     9301 acgttccgcc tccagacgaa gagaattctg tttgaaacga tacagacctc cagagcctag
     9361 tcctatggtt tgatgtgtct tgtctatcga atacccttga ccaaagaaga tggaccctaa
     9421 atcctgaaga atcttttgaa cggtgtcgtt tggaatgggc gagtttcctc ccagcatatt
     9481 cattcccagg gcgagtcgag tcaaattttg ttgcaattgg gaatctttta aatcgacgag
     9541 ccgatcggga ttgcgttcgc catacagggt ttccaataat acggacaggt tccccaattc
     9601 tgtctgaata atcgacacgc ctagggcggc atggcgaatc tgatgagcga gcaagtcatc
     9661 ggcgatctcc aatgcttccg aaggagaggc ctgttttcct ttgcggtagg ccatggtttg
     9721 tgctaggcgt gtcttctctt cccaggtttg aatttggttt ctcaagtttt tcaaccgttc
     9781 ccgagccatt tttatggtct gctgtgattg caggtctttc aagattggcc cggaatgttg
     9841 gacccacttt cgcacctcca cgccgagggc ttgcagacgc tgaccagttt ttagcatttg
     9901 actcaaaatg tcgtgggtgt ctccggtggg gtattggtct agtcgctcac tcaacgcatg
     9961 tacgtaggat tgaaatggat cggggttgag tatgctctca acaggattct ccagggtgtt
    10021 ttccatttgt ttcactgcct gcacaccgag atgattcagc gcattaccca tttccctctg
    10081 taacgctcct gcttccatcg caactatttt gttggtttgt acctcaaaga gcgtatatct
    10141 cgtcaggcct agggtgacaa caccaaggag cacaccaagg aatacgagaa accacacgaa
    10201 ccaaaggacg cgatgtccag tcggagcgaa tgtcatggcg aacttaatgg gccttcacga
    10261 gttgaggtca gtctttcaaa ttaaaggaga cgaccgttcc ctttttcaat tgagtcgctc
    10321 agagcatctg gctggtctgg taatgggagg cgttcaattg aagaggatgt ttcattccta
    10381 aatcgaaggt tcctaattgt tccagggcgg atacagcagt gactcgtgtg atgatcggtc
    10441 tgcattcttc tggaccgaat tccgaagcta agggaatatt gcccaacaag g
//
LOCUS       DATIAL010000423         7384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1044375, whole genome shotgun sequence.
ACCESSION   DATIAL010000423 DATIAL010000000
VERSION     DATIAL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044375"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1008
                     /locus_tag="VLA60_13160"
     CDS             31..1008
                     /locus_tag="VLA60_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HSF10357.1"
                     /translation="MEREDALQDITIEIPKAEGRDLLVKIIAVSVNPVDTKLRLRAAP
                     EGDWGVLGFDASGVVEAVGPEVQNFKPGHAVFYAGSIARPGTNSEYHLVDERIVGRKP
                     ASLSDAEAAALPLTAITAWEMLFDRLDVKRPTPQGGNAILIIGGAGGVGSITIQLLRT
                     LTDLTVVATASRPETQDWVRECGAHHVFDHRQPLAPQVEALGLGAPGFVFSTTQTGQH
                     LADIVGLIAPQGRFGLIDDPDVLNVMPFKLKAVSIHWEMMFTRSLFGTPDMAEQGKLL
                     NEVAALVEAGRIRSTVTEVAGKIDAATLRAVHAQLESGSARGKIVLEGF"
     gene            1005..1160
                     /locus_tag="VLA60_13165"
     CDS             1005..1160
                     /locus_tag="VLA60_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10358.1"
                     /translation="MIRCVAEANPPPWSADMPDKLEANTHPAVVFYRTAYLANPAKVV
                     ELYVGVE"
     gene            1993..3483
                     /locus_tag="VLA60_13170"
     CDS             1993..3483
                     /locus_tag="VLA60_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HSF10359.1"
                     /translation="MARQRVTAQDLYNYTKCLHRVYLDSNGDPSEKSEVSSFVKLLWE
                     VGLQTERDYITSLGDQEIVDLQNLAVEPAFQETILAMEHGAAVIYQGCLIHERFVGRP
                     DLLVKREDGASRFGKYLYEPIDIKAGKGWEEADGKKPRFKEHYAFQILFYRLLLEQIQ
                     GTVTSGGRIINVDKQLEEFDPAPFEERFNQAMQDVQQLVSGEETSEPVLGSHCTMCGW
                     FSRCYRWVKDESDPTGLFFVGKQKFALREQGLRTIQDIAKMDVQTYLKPPHKIPRMGK
                     VSLTRMKERAQVLLEGKPRIRSGYSFPEVVREIYFDIEDDPTRGVTYLFGMVIQDGKS
                     PSHFTYFLAKRPEDEEQTVRDFWQFIQSTDKAVYYVYSHKERSTLKRLMEKYDLDADT
                     FEKYKSCEFDLYQDLIVEYSDWPTFSYGIKHIAKFIGFQWRDVDPSGANSIAWYNDYL
                     ANPANEALLNRILEYNEDDCYAMAAITRYFEHHAHKTQGMAKKLAP"
     gene            3509..4201
                     /locus_tag="VLA60_13175"
     CDS             3509..4201
                     /locus_tag="VLA60_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10360.1"
                     /translation="MAATVLVVDDEKDLVELVKYHLEKEGLKCLEARDGETALQLARE
                     RTPDLMVLDLMLPGVDGLEVCRKLRKDPKTSSIAIIMLTAKAEEVDRIVGLEMGADDY
                     MVKPFSPRELLARVKAVLRRGQGQDMPTLKRVGTLEVDEGKHQVTVEGTVVELTVKEF
                     DLLCALMRANGRVLNREQILETVWGYSNAVDIESRTVDVHIRRLREKLGSECTRIVTV
                     KGVGYRFESGSV"
     gene            4231..5613
                     /locus_tag="VLA60_13180"
     CDS             4231..5613
                     /locus_tag="VLA60_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10361.1"
                     /translation="MRNPSLILAGWMVSAMIGAGVAVAWAWPFWVMVLLGAGLVGLAV
                     LLTRDIWDQTRENHEDVMTGVRQLEQLSGIDEARTSRVVESDALGGHIQRLVENVKKR
                     MTGLEEERTKIIAIMENLVEGVVAFDPKGKVLFTNSSAHRILGLEAQAIQGRSLWEII
                     RNQELAGLVEGCQELAWHERRQAEVELHPPVSMVLEVYALPFPFSNQKKGSVLVLHDV
                     TQLRRLEQVRTEFVENVSHELRTPLTAIVGYLETLVDEPSLDTPNNRKFVQVAHQHAV
                     RLSRLVEDLRSLSEIESGKVVLRCESVPLGEVAQDVCEMFQHQLSKKNLQVSNKIGVE
                     RSAWVDRDRLIQILVNLVDNAIKYTSAGGTISFQAIPVGDARISLQLKDTGQGIPSTD
                     LPRITERFYRVDRARSREEGGTGLGLSIVKHLLQLLDGKLRIQSELGKGTTVEVILPI
                     SRPTPSSQSL"
     gene            5812..6123
                     /locus_tag="VLA60_13185"
     CDS             5812..6123
                     /locus_tag="VLA60_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10362.1"
                     /translation="MGTTSTEWLAPVDVEGKDHDQVLAVISASPGVTIEELLEHLPWM
                     RWGHLFSILGECLQEGSVILCQKEFQFEVRAIHSSSSGNEKDVLTGYMPSKSGDRWMG
                     I"
     gene            6201..7214
                     /locus_tag="VLA60_13190"
     CDS             6201..7214
                     /locus_tag="VLA60_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF10363.1"
                     /translation="MKHNQKRRKMWVLGLGIGLFLGWTGTAILGVSPVLAKDEMEDPD
                     WVNYAKVSGVSGNLNSIGSDTLNNLMTLWAEGFRKQYPNVKIQIEGKGSSTAPPALIE
                     GTAQLGPMSRKMKGKEIDAFESKFGYKPTAFPVAVDALAVYVNKDNPIQGMTIEQVDA
                     VFSKTRQHGYKENLSSWGQLGLTGMFADMPISIYGRNSASGTYGFFKEVVLENGDYKD
                     EVKEQPGSASVVQGVTEDQAGIGYSGIGYRTSSVHALPLSMKAGQPFVEPTYENATSG
                     EYPLSRYLFVYVNKAPNQPLSPIVREFLKYVYSREGQKVVIKDGYFPISEKIIGKQSE
                     HLK"
BASE COUNT         1832 a         1634 c         2098 g         1820 t
ORIGIN      
        1 aaagccgttg cctacaaaac tcctggtccg attgagcgtg aagacgccct ccaggatatc
       61 accattgaga tacccaaagc cgaaggccgt gatctactcg tcaagatcat cgctgtgtcg
      121 gtcaaccccg tggacaccaa attgcgcctc cgtgctgcgc ccgaaggcga ttggggagtc
      181 cttggatttg atgcctctgg cgttgtcgag gccgtagggc ctgaggtgca gaattttaaa
      241 ccgggccatg cggtttttta tgccggatcc atcgcgcgac ccggcaccaa cagcgaatat
      301 caccttgtcg atgaacgcat tgttggccgc aaacctgcca gcctgagcga cgcggaggcc
      361 gcagcacttc cgctcacggc gatcaccgcc tgggaaatgc tgtttgaccg gctcgatgtc
      421 aaacgcccga ccccgcaggg aggcaacgcc atcttaatca ttggtggcgc aggcggcgtg
      481 ggctcgatca ccatccagct tctgcggaca ttgaccgatc tgacggtagt cgccacggcc
      541 tcccgtcctg aaacacagga ttgggtgcgc gaatgcggcg cccatcacgt attcgaccac
      601 cgccagcctt tagcacctca ggttgaggca ctgggtctcg gcgctcccgg ttttgtgttc
      661 tcaacgactc agaccggcca gcatctagcc gatatcgtcg gactgattgc gccgcagggc
      721 cgttttggcc tgatcgacga cccggacgtg ctcaacgtca tgcccttcaa gctcaaggcc
      781 gtatcaattc attgggaaat gatgttcacg cgttcgctct ttggcacacc ggatatggcg
      841 gaacagggaa agctgttgaa cgaagtggct gcgctggtcg aggcgggacg catccgttcc
      901 accgtgaccg aagtcgcggg caagatcgat gcggcgaccc tgcgcgcagt gcatgcgcag
      961 ctcgaaagcg gctcggcgcg gggcaagatt gtgcttgaag ggttttgatt cgctgcgtag
     1021 cagaggcgaa tcccccccct tggagtgccg acatgccgga caagctggaa gcgaatacgc
     1081 atcccgccgt ggtcttctac cgcacggcgt acttggctaa tcccgccaaa gttgtcgaac
     1141 tgtacgtcgg tgttgaatag atttaacaca attcactcgt agagggggaa gcgggcgttt
     1201 tttgattatt ccatggaaat ggcggagcaa tgccccggca agaagatgga gttcgcctgt
     1261 gcggtagcca ttgagacctc gtcgctttat acaaccatcc gacgtggcca aacaatgaag
     1321 catacgtgac gatggatttc tttcgattcg attaggaggg aaagatcctt tagcattggg
     1381 ataccattca agaagttcca actgaaacaa aaaacggaaa tatgagtaca agcggcacta
     1441 ctgctcaaaa tcgcaggtaa cgatcttata gccgtgcagg gcgacgaaat gattctcaaa
     1501 tccgtggtaa tgcttcttgt ttgaggtgtt tgaagaagtg ccgaagatat gaacggacgg
     1561 atttgattaa aaatcttatt ccagcttccc aatacataaa ttgaaatcct cctctgatta
     1621 tgggacatgg ggcccaaccg gaaaaattag gatgagaaaa ttttcgggtt caattattta
     1681 tctaataata ggaagagtaa tagtcggatg ttgccggaag gttaggcctg gattacatca
     1741 atgtaaacaa tgcacaaata gaaggcttga aatggaggac tagcaggtct ccaggatctc
     1801 gaaacgcttt aatagggatc gaggctacga ctgccgtatc aaccgaagag tttcggtgaa
     1861 aattttctcc cttgcgttct ttgactcctt tacatttccg ttcggtcttt tctgcctgtt
     1921 gtggtagtat atctcgacag tgaatgcaga attatcccca atgtgaacat ctgcttcgtt
     1981 ccttcctgaa acatggcccg gcaacgagtc accgcacaag acctctataa ctacaccaag
     2041 tgtttgcatc gcgtgtatct ggatagcaat ggcgatcctt cggagaaatc cgaggtcagc
     2101 tcatttgtga agctgttgtg ggaagtgggg ttgcagactg aacgggatta tattacttcg
     2161 ctcggcgatc aggagatcgt ggacttgcaa aatcttgcgg tagagccggc atttcaggaa
     2221 accatcctgg ctatggagca tggagccgct gtgatttatc aaggctgtct gatccatgag
     2281 cgctttgtgg ggcgaccgga tttattggtg aaacgtgagg atggagcgtc tcgctttggc
     2341 aaatatcttt atgagccgat tgatattaaa gctggaaagg gttgggagga ggcggacggc
     2401 aagaaaccca ggtttaaaga acattacgcc tttcagattt tgttttatcg gctgttactt
     2461 gagcagattc agggaacagt tacatcgggt ggccggatta ttaatgtgga taaacagctt
     2521 gaagaatttg atccggctcc ctttgaagaa cgatttaacc aggccatgca ggacgttcag
     2581 caactggtgt ccggagagga gacttcggag ccggtgctgg gaagtcactg caccatgtgc
     2641 ggatggttta gccggtgtta ccgatgggtg aaagacgagt ctgatcccac cggtttattt
     2701 tttgtgggca agcaaaaatt tgcactccgg gagcagggac tccgaacgat tcaagatatt
     2761 gccaaaatgg atgtgcaaac ctatctcaag ccgcctcaca agattccgcg aatgggaaaa
     2821 gtctccctga cccgaatgaa agagcgggcg caggttctgt tagaggggaa gccgaggata
     2881 cgctccggct atagttttcc ggaggttgtc cgggagatct attttgatat tgaagatgac
     2941 ccgacccgtg gcgtcaccta tctgttcgga atggtcattc aggatgggaa aagtccctct
     3001 cattttacct attttttggc taaaaggccc gaagacgaag agcagacggt cagggatttc
     3061 tggcaattta ttcaatcgac ggataaagct gtgtattatg tgtattccca taaggagcga
     3121 tcgacgctga aacgcctgat ggaaaaatat gatctggatg ccgatacctt tgaaaaatat
     3181 aagtcctgtg aatttgattt gtatcaagac ctcattgtgg aatactctga ttggccgaca
     3241 ttttcttatg ggattaaaca tatcgccaag tttatcgggt ttcagtggag agacgtggac
     3301 ccgtccggcg ccaactccat tgcctggtat aacgattatt tagccaatcc cgccaatgaa
     3361 gcgctgctga atagaatttt ggaatataat gaggatgatt gttatgccat ggcagccatc
     3421 acacgatatt ttgaacatca cgcccacaag actcagggta tggctaaaaa gctggcccct
     3481 tgagacgcaa tgttgaggag attggactat ggctgcaacg gtattagtcg ttgatgatga
     3541 aaaggatctg gtggagttgg tcaagtatca cttggaaaag gagggactca agtgcttgga
     3601 agcgcgtgac ggggaaacgg ccttgcagct ggccagggaa cgtactccgg atcttatggt
     3661 cctggacctg atgttgccgg gggttgatgg gttagaggtc tgccggaagt tgaggaaaga
     3721 cccgaaaacg tcttcgatcg ctattattat gctgacggcg aaagccgaag aagtggatcg
     3781 gattgtcggg ttggaaatgg gggcggacga ctatatggtg aaacccttta gtccgaggga
     3841 actccttgcc agagtgaaag ccgtgttacg gcggggacag gggcaggaca tgccgaccct
     3901 caaacgggtt ggaaccctcg aggtcgatga aggcaagcat caggtgacgg tggagggaac
     3961 cgtggtggag ttaaccgtca aggaattcga tctgctctgt gcgttgatgc gggcgaatgg
     4021 ccgggtcctg aaccgggagc aaatattgga aacagtttgg ggctattcca atgccgtgga
     4081 tattgaatca cgaacggtgg atgtgcacat tcggcggctc cgggaaaagt taggaagcga
     4141 atgcacacga attgtgacgg tcaaaggtgt gggctatcgt tttgaatcgg ggtcggtcta
     4201 aggtgaatat gatgaacgtg accttcatca atgcgtaatc cctcgctcat tcttgctggc
     4261 tggatggtgt cggctatgat cggggcgggt gtggcggttg cctgggcatg gccattctgg
     4321 gtgatggtct tattgggggc tggcctggtt ggcctggcgg tgttgctgac ccgggacatc
     4381 tgggatcaga cgagagaaaa tcatgaagat gtcatgacag gcgttcgtca acttgaacag
     4441 ctcagcggaa ttgatgaggc gaggacttct cgtgtggtgg aatccgatgc cttggggggc
     4501 catatccagc gcctggtgga aaacgtgaag aaacgtatga ccggcctcga ggaagaacgc
     4561 acgaaaatta tcgccatcat ggaaaatctg gtggaaggag tggtggcctt cgatccaaag
     4621 ggaaaggtgt tgttcaccaa ttccagcgcc catcgaatat tgggattgga ggctcaggct
     4681 attcaaggac ggtcgctgtg ggaaatcatt agaaatcaag aactggcagg tctggttgaa
     4741 ggttgtcagg aattggcgtg gcatgagcga cgacaggcag aggtagaatt gcatcctcct
     4801 gtctccatgg tgttagaggt ctatgcgctc ccttttccct tttccaatca gaagaagggg
     4861 agtgtgctgg tgttgcacga tgtcacccaa ttgcgtcgct tggaacaggt acggacggag
     4921 tttgttgaga acgtgtccca tgaactgcgc accccgctga cggccattgt cggatatttg
     4981 gaaacgcttg tggatgaacc gtcattggac actccgaaca atcggaaatt tgtgcaggtt
     5041 gcccaccaac atgcggtgcg gctcagtcgg ttagtagaag acttacgaag cctctcggaa
     5101 attgaatcgg gaaaagtcgt attgcgttgt gaatcggtgc cgttgggtga agtggcgcag
     5161 gatgtctgtg agatgtttca acatcaactg tcgaaaaaaa atctgcaggt ttccaataag
     5221 atcggagtcg aacgtagtgc ttgggttgat agagatcggt tgattcagat tctagtgaat
     5281 ctcgtggata atgccattaa atatacgtct gccggaggca ccatatcgtt tcaagccatc
     5341 cctgtgggtg atgcgcggat ctcactgcag ctcaaagata caggccaggg tattccttcg
     5401 acggatcttc cgaggattac cgaacgattt tatcgggtgg atcgagcgcg gtcccgtgaa
     5461 gaaggcggga cgggccttgg gctctccatc gtcaagcatc ttcttcaact gctcgatggc
     5521 aaacttcgca tacagagtga attgggtaaa ggcaccaccg tggaggtgat cctgccaata
     5581 tccaggccca ctccctcaag tcaatctttg tgagatttcg tgttctctcc cgggtaactc
     5641 ccttcctcta gttttttcac ctaatccccc caaaaaaacg ttacacagtt gtaacccgaa
     5701 cctcacagag gtgaaatagt cattcgctag gattcggtgg cagggttaaa taattagact
     5761 gccgagcttt ggaactgtct caaggtgaga cgacaatgac gggagggact tatgggaaca
     5821 actagcacag aatggcttgc tccggttgat gtggaaggga aagatcatga tcaggtgtta
     5881 gcggtcattt ctgccagtcc gggtgtgacg atagaagaac ttttggagca tttgccatgg
     5941 atgcggtggg ggcatttatt ttccatcctt ggggaatgcc tgcaagaagg gtcggtaatt
     6001 ctgtgccaga aggagtttca atttgaagtc cgggccatcc attcgtcgtc aagcggaaac
     6061 gagaaggatg ttctgacggg ctatatgccg tcaaaatcgg gagatcggtg gatgggtatt
     6121 taacacgttc gtaacattgc cgttacatag aggaaatctg ttagacctac actatttatt
     6181 cacttgagga ggcacgattg atgaaacaca atcagaaacg aaggaaaatg tgggtattgg
     6241 gcttgggcat aggactgttc ctgggctgga ccgggaccgc gatcctcggg gtttctccag
     6301 ttttggctaa ggatgagatg gaagatcctg attgggtgaa ttatgccaag gtgagtggcg
     6361 tatccggaaa tctcaatagc attggctcgg ataccctgaa taatcttatg acgctgtggg
     6421 ctgaaggatt ccggaagcaa tatccgaatg tcaaaattca aattgaggga aaaggatcaa
     6481 gcactgctcc ccctgctctt attgaaggaa cggctcaatt ggggccgatg tctcgtaaaa
     6541 tgaaggggaa agaaatcgat gcctttgaat cgaagttcgg ttataaacct accgcctttc
     6601 ccgttgcggt agatgcattg gcggtctatg tcaataagga taatcccatt caagggatga
     6661 cgattgagca agtcgacgcg gtgttttcca agacccggca gcacggatat aaggaaaacc
     6721 tttccagttg gggccaattg ggcttaaccg gaatgtttgc cgatatgccg atcagcatct
     6781 atggccggaa ttcggcgtcg gggacctatg ggtttttcaa agaagtggtg ctggaaaatg
     6841 gtgattataa agatgaagtg aaggaacagc ccggttcggc ttctgtcgtg caaggggtga
     6901 ctgaagatca ggcagggatc ggatatagcg gtattggata tcgaacttct agtgtccatg
     6961 cgcttcctct ttccatgaag gcagggcaac cctttgtgga gcccacctat gaaaatgcca
     7021 ccagcgggga gtaccccttg tcacggtatt tatttgtgta tgtgaacaaa gctcctaatc
     7081 aaccgttgtc gccgattgtt cgagaatttt tgaaatatgt gtatagtcgt gaaggacaga
     7141 aggtcgtcat taaagatggc tatttcccca tctcggaaaa gattattggg aaacagtcgg
     7201 agcacctcaa gtaggaatag cttaaggaac atcctgaccg cctcccggag aaatcttttg
     7261 gagagcggtc gggtgttctg atatcatttc agccctgttt ctcatttctc cttcccaacg
     7321 accacatatg atgccacccg tatcttcccc ctcctctttg gaatctaata gacaatcgtt
     7381 ggtt
//
LOCUS       DATIAL010000424         7520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_615038, whole genome shotgun sequence.
ACCESSION   DATIAL010000424 DATIAL010000000
VERSION     DATIAL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615038"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2468)
                     /locus_tag="VLA60_13195"
     CDS             complement(<1..2468)
                     /locus_tag="VLA60_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HSF10364.1"
                     /translation="MSVSDRKKPDVCWHAMDGESVLKAVASQVGGLTQDVAAQRLALY
                     GPNRLRPPKKQSSWQRFLAQFHNILIYILLAAGVVTAILGHWVDTSVILGVVIINAVI
                     GFLQEGKAERAMDAIRRMLSVQACGLRDGTRCPIPAESLVPGDIVFLQSGDKVPADLR
                     LLKVKDLRIEEAALTGESVPVDKHMKPVPETASIGDRRGMAYSGTLVTYGTGTGVVVA
                     TGDASEIGRISAMLARVQTLTTPLLRKIGEFGSRLSIAIITGAIGVFLFGVFFREYGF
                     SDMFLASVGLAVAAIPEGLPAIMTITLAIGVQAMARRQAIIRRLPAVETLGSVTVICS
                     DKTGTLTRNEMTVQTLATAAYTVEVSGVGYDPHGGFSLLGNPIVLTDLPDVMELARAG
                     MLCNDADLDHVEGVWQINGDPTEGALVTLGRKAGLDYRFEHEMWPRTDVIPFESQHRF
                     MSTLHHDHAGHGFMYIKGAPERVLEMCAFQRSLGENHPLNSRSWHDRIEQMANRGQRV
                     LAVAVASTNHEHRELRFRDVEQGLTLLGLFGIVDPPRPEAIEAVRECQQAGIRVKMIT
                     GDHLVTARTIGLQMHIGDGKQALSGQVLETLSDEELKRVVRDIDIFARTSPEHKLRLV
                     QALQAGGEVVAMTGDGVNDAPALKRADVGVAMGVKGTEVAKEAAEMVLADDNFASIAH
                     AVEEGRRVYDNIQKSILFILPTNVAEAGVIVAAILLGTMLPITPVQILWVNMITAVTL
                     ALSLTVEPPESDVMRRPPRSPGEAILSRFLLWRIGFVSTLAVAGTFGLFLWETDQGAS
                     IETARTIAVNMLVVFEAFYLLNARS"
     gene            complement(2619..3563)
                     /locus_tag="VLA60_13200"
     CDS             complement(2619..3563)
                     /locus_tag="VLA60_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF10365.1"
                     /translation="MKILVAVDPSEHAEEAIRFVKSVDWPKASEIYLIHVIEMKHASP
                     LIPSGGPSSWDRVISEARGKLFTEAKGLLEHTRKEILEERAVIIKSLVMEGLPGAQIL
                     QAVEDYQIDLVILGTRGLSNVKRFLLGSTSDWVMREAPCSVLLVREKLSKVTMGKSAA
                     KILLATDGSAVALSTVDMLGLLTCKTPPKVTVAHVVGRPAYLEGWYWGKGKAAFKQLA
                     EQVSENAQKEGASHLEEVSQRVKGLGMEVDTVLTKGDPAEEIVKFAERSKAKLIMVGS
                     KGFIGGRPVPLGGVVRKIARYAPCSVLLTRPGRSEKKG"
     gene            complement(3620..5152)
                     /gene="malQ"
                     /locus_tag="VLA60_13205"
     CDS             complement(3620..5152)
                     /gene="malQ"
                     /locus_tag="VLA60_13205"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HSF10366.1"
                     /translation="MVNLARGSGILLHPTSLPEGWGIGDLGPTAYQFVEFLKAAGQSW
                     WQMLPLGPTGYGNSPYMCFSAFAGNPLLISPEKLVEDGFVSASDAGRPPSFSADRVDY
                     PLVIAHKRLIYEKSFEQFQANPPAEHRQAFMLFREKHASWLEDFSLFMSLKESYAGQA
                     WTRWDKPLIVRDPQALQDCCRRLREKIDYHQFLQYLFFHQWSALRQYAHAEGVRIIGD
                     LPIYIAHDSSDVWAHPDSFYLDDQCQPSVVAGVPPDYFSATGQRWGNPIYRWDVRAGS
                     GYQWWIDRFRANLALVDMIRLDHFRGFEAYWEIPASEPHAIHGRWVKGPGGELFAAVK
                     AALGEVPVIAEDLGVITPEVEALRDTCGFPGMRILQMAFGNDPKAHHYRPHHYTQNSI
                     VYTATHDHNTTVGWFTAEPGKETTQSKEEIKEERVSVLRYLGTDGRDIHWDVIRLAMS
                     SVAQLAMVPLQDVLGLGSECRMNRPGTLKGNWEWRIHPGQLTEKVGSRLRDLTGLFDR
                     LP"
     gene            complement(5290..5853)
                     /locus_tag="VLA60_13210"
     CDS             complement(5290..5853)
                     /locus_tag="VLA60_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10367.1"
                     /translation="MIMIVTFHEEIRGKISAFLSEKGYEVCVPPHRQDVIPLVKEKSP
                     LVIVLDMYVAAPNGLDVLKELRAQHYHGRIVALAGTSVRSLMSQASQLGVDQVIGGFQ
                     GDGGALNLDQVESAIKMALHSSIAKRAFELYVAGGRTKGKDLDDWLEAEREIFSKTIS
                     LPSEEREVKAEPESAPKKRKKSPKKLS"
     gene            complement(6714..7082)
                     /locus_tag="VLA60_13215"
     CDS             complement(6714..7082)
                     /locus_tag="VLA60_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10368.1"
                     /translation="MTSFHKILCFEVWLLLVLVAGCYESPTETVSEDAGVEHPGFIGK
                     VSETVNADISGPGVATYLRSQNTVDGVRSGYYLIANDRGTKDPLVTFRIPTGTESGMY
                     ELVAADPMELGENFEVQLRA"
BASE COUNT         1885 a         2177 c         1672 g         1786 t
ORIGIN      
        1 gaccgggcat ttaacaaata aaaggcttca aagaccacca gcatgttgac ggctatcgtc
       61 ctcgcggttt cgatagatgc cccctggtca gtctcccata ggaacagtcc aaacgtgccg
      121 gccaccgcta aggtggatac aaagcctatt cgccacagca gaaaccgtga caaaatggct
      181 tcccctgggc ttcgaggggg acgtcgcatg acatccgatt caggaggttc gacggttaag
      241 gacaaggcca atgtcacggc ggtgatcatg ttgacccaca ggatttgtac cggcgtaatc
      301 ggcagcatgg tccctaacag aatagcggcc acaatcactc cggcttcggc gacattggtg
      361 ggaagaataa acagaatgga cttttgaata ttgtcataaa cccgacggcc ttcctcaacg
      421 gcatgcgcaa tcgaggcaaa attatcatcg gccagcacca tttccgccgc ttccttcgcc
      481 acttccgtcc cctttactcc catggccact ccgacatccg cccgcttgag tgccggcgca
      541 tcattcaccc catccccggt catggccacc acttccccac cggcctggag ggcttgcaca
      601 agccgtaact tatgctccgg actcgtccgg gcaaagatat cgatatccct taccacacgc
      661 ttcaattcct catcgctcaa cgtttccagg acctgtcccg agagagcctg tttcccatct
      721 ccaatatgca tttgtaagcc aatcgttctg gccgtcacca gatgatcccc ggtaatcatt
      781 ttgactcgaa tcccggcttg ttggcattct cggaccgctt caatggcttc aggccgtggt
      841 gggtcaacaa tgccaaagag gcctaacagt gtcaggccct gctccacatc tcgaaaccgt
      901 aactctcgat gttcgtgatt ggtggatgca acggccaccg ccagcactcg ttgtccacga
      961 ttcgccatct gttcgatccg atcatgccac gaccggctgt tcagaggatg attctctccc
     1021 aaactccgct gaaacgcgca catctccaac acccgttcag gggcaccctt gatatacata
     1081 aatccatgcc cggcatggtc gtgatgcaac gttgacataa accgatgttg cgattcaaag
     1141 gggatcacat ccgttcgtgg ccacatctca tgttcgaatc ggtaatccaa tccggctttt
     1201 ctcccgaggg tcaccaacgc cccctccgtc ggatctccgt tgatctgcca gaccccctcc
     1261 acgtggtcca gatccgcatc gttacacaac attccggctc gagcgagttc catcacgtcc
     1321 ggaagatcag tcagcacgat cgggttgcca agcagagaaa atcctccatg cggatcatag
     1381 ccgacaccac tgacctccac ggtatacgcc gcggtcgcca gcgtttgaac ggtcatttca
     1441 tttcgtgtca gcgtgccggt tttatctgaa caaataacgg tcacagaccc aagggtttca
     1501 acagccggca gccgtcgaat aatcgcttga cgtcgggcca tcgcttgaac cccgatggcc
     1561 agcgtgatgg tcataatggc cggaaggcct tcgggaatgg cggcaacggc taatcctacg
     1621 ctggccagaa acatatcact gaatccatac tcccggaaaa acactccgaa gaggaacaca
     1681 ccaatcgctc cggtgataat ggcaatactc aatcggctcc cgaattctcc gatctttcgg
     1741 agaagtgggg tggtcagggt ctgcaccctg gccagcatgg cgctgatccg tccaatttcg
     1801 gaggcatctc cggtcgcaac caccaccccg gttcccgttc cataggtcac cagagttccc
     1861 gaataggcca tgccccttcg atcaccaatg gacgcggttt ccgggaccgg tttcatgtgt
     1921 ttgtcaaccg ggacggactc accggtcaag gcggcttctt caatacgcaa atctttcacc
     1981 ttcagaagac gcagatccgc cggaactttg tctccggatt gcagaaacac aatatccccc
     2041 ggcacaaggc tttccgcggg aatcggacaa cgggttccat cacgaagtcc acaggcctgc
     2101 accgacaaca tccggcgaat ggcatccatc gccctttcgg ctttgccttc ctgaagaaat
     2161 ccgataacgg cattgatgat caccacgccc agaatcacac tggtatccac ccaatgaccc
     2221 agaatggcgg tcacgacacc cgctgccagg aggatataaa ttaaaatatt gtggaactgg
     2281 gcgagaaacc gctgccagga tgattgcttt ttaggcggac gtagacgatt ggggccatag
     2341 agagccagcc gttgggcagc aacatcctgt gttaagcccc caacttgact ggccactgcc
     2401 ttgaggacgg attctccatc catggcatgc cagcaaacgt ctggcttttt tcgatcggat
     2461 accgacatcg cgctcctttt aattgaggat aggaccacac atgagcaaca ggttcaacat
     2521 taacatttac ccaccacact gccggacgac catgacaatt gcgtccttta ccctttgatg
     2581 gccttgagtc ttcctgaaga gggaaaatgg atcacgcatc aacctttttt ctcggatctg
     2641 ccaggccggg tgagcaacac cgaacatgga gcatagcgag caatttttct gacgacccct
     2701 cccaatggga ccggcctgcc ccctatgaat ccttttgatc caaccatgat caattttgcc
     2761 ttcgaacgct cggcaaattt gacaatttct tcagcaggat ctcctttggt caacactgta
     2821 tcgacttcca tgccgagtcc tttgactctt tggctcacct cttccaggtg gctggcacct
     2881 tctttttgag cgttctccga tacttgttcg gctaattgtt taaatgccgc ttttcctttt
     2941 ccccaatacc aaccctccag gtaagccggt cttcccacca catgcgccac cgtcaccttt
     3001 ggaggggtct tgcaggtcaa cagaccgagc atatccacag tactcaaagc caccgctgac
     3061 ccatcagtgg ctaaaagaat tttggcggcg gattttccca tcgtcacctt actaagtttt
     3121 tcacgaacca ataacactga acacggggct tccctcatca cccaatcact ggtactccct
     3181 agaagaaacc gtttcacatt ggaaaggcca cgcgtcccta agatgaccag gtcaatttga
     3241 tagtcctcca ccgcctgtaa aatttgcgca cccggaagcc cttccatcac cagggactta
     3301 atgatcacag ccctttcttc aagaatctcc ttcctggtgt gctcaagaag acccttcgct
     3361 tcggtgaaca gtttccccct tgcttcggaa atgacccgat cccaactgga cggtccaccc
     3421 gacggtatca gaggagacgc atgtttcatc tcgatcacat ggattaaata gatttctgat
     3481 gcctttggcc aatccaccga tttcacaaat cgaatggcct cctctgcatg ttcggaagga
     3541 tcaaccgcca ctaaaatttt catcgtcatc tccctttctc acaaaagctg taattttttt
     3601 tggattcctt aggcgtgggt catggcaatc gatcaaataa acctgtcagg tcacggagtc
     3661 ggctcccaac cttctccgtc aattgcccgg gatgaatccg ccactcccag ttccccttga
     3721 gcgtgccggg gcgattcatc cggcattcac tacccaatcc caaaacgtcc tgaaggggaa
     3781 ccatggcgag ttgagcaaca gaactcatgg ccagtcgaat cacatcccaa tgaatgtcac
     3841 gcccgtcggt cccaagatag cgcaacacac tcacacgctc ctccttgatc tcttctttgg
     3901 attgggtggt ttccttcccc ggttcagcgg taaaccaccc gaccgtcgta ttatggtcat
     3961 gcgtggccgt atagacgatg gaattttggg tatagtgatg gggtcgataa tgatgggcct
     4021 tgggatcgtt gccaaaggcc atttgtaaaa ttcgcatacc gggaaaacca caggtatccc
     4081 gcaacgcctc cacctcggga gtgatcaccc caaggtcttc ggcgatcacc ggcacctcac
     4141 ccagtgcggc tttcacggct gcaaacaatt cgcctcccgg acctttcacc catcgaccat
     4201 ggatggcatg aggctcagaa gccgggattt cccaataggc ttcaaaccct cggaaatgat
     4261 ccaatcgaat catatcgaca agagcaagat tggcacggaa tcgatcgatc caccattggt
     4321 aaccggaccc ggctctcacg tcccaccggt aaatcggatt tccccaccgt tgtcctgttg
     4381 cgctgaaata gtccggcggc accccggcca ccacggacgg ttgacattga tcatccaaat
     4441 agaatgaatc aggatgcgcc cagacgtctg aactatcatg cgcgatatag atcgggaggt
     4501 ccccgataat tctgaccccc tcggcatgag catactgtct cagggcggac cattggtgaa
     4561 aaaacagata ctgaagaaac tgatgataat caatcttctc cctgaggcga cgacagcagt
     4621 cctgcagggc ttgaggatct cgaacgatta gtggcttatc ccatcgagtc catgcttgcc
     4681 ccgcatagct ttctttcaaa gacataaaga gggagaaatc ttctaaccag gaagcatgct
     4741 tctcacgaaa caacataaag gcttggcgat gttcggctgg agggttggcc tgaaactgct
     4801 caaaagactt ttcgtagatt agtcgcttat gcgcaatgac cagagggtaa tccacacggt
     4861 cggctgaaaa agacggaggc cgccccgcat cggatgccga tacaaatcca tcctccacaa
     4921 gtttttccgg gctaatgagt agggggtttc ctgcaaaggc ggaaaagcac atgtaggggg
     4981 aattgccata cccggtcggt cctagtggca acatctgcca ccaactctga ccggcggcct
     5041 ttaaaaattc gacaaattga taggccgtgg gtcctaaatc cccaatgccc catccctccg
     5101 ggagagacgt cggatgcagt aagattccac ttcctctcgc cagatttacc atgatcccgg
     5161 actgaatgct ctgaaaacca atttaattga aagggtagga ttaaaattcc gttttttttg
     5221 tgttcaatga cattcgcaaa ccattcttca gacgtccagc cgtcccttgt aagttggaac
     5281 tcaggccggt caagataatt tctttggaga ctttttccgc tttttgggtg cactttcagg
     5341 ctcagccttt acttcacgtt cctctgatgg caacgaaatg gtcttactaa aaatttcccg
     5401 ttcagcctca agccaatcgt ccaggtcctt ccctttcgtc cgacctcctg ccacatataa
     5461 ttcaaatgcg cgtttggcaa tgcttgagtg taatgccatt ttaatcgcag actccacctg
     5521 atcaagattc aatgccccgc catcgccttg aaatcctcca atcacctggt ccacgccgag
     5581 ctgggacgcc tgagacatta aggaacgcac cgaggttccg gccagcgcca caattcttcc
     5641 atgataatgt tgggctcgta gttcctttag cacgtctagt ccgtttggtg ccgccacata
     5701 catatccaac acgatcacta agggggattt ttcctttacc aggggaatca catcctgcct
     5761 atgaggagga acacagactt catagccttt ctcagagaga aaagcggaaa tttttcctcg
     5821 aatttcttcg tgaaatgtca caatcataat catcgagata taacctccct atcacaatcc
     5881 caaggatcga ctcagccacg cttccatgat acagagtaca aattctatac cattttcatt
     5941 tcgtaaatac attcatgagc tttccctgca ctcacacagc actgtgagtg gctgtctaga
     6001 aagggaaaat cctcatagag gcaaattgcc ccatattcgg acagtgctgg aaccccttgt
     6061 agaggaattg ctccacacaa ttttcctgcc cggatattta tttttggcag actcatactc
     6121 ctcccacaaa acaccacttc cgccagcccg tgattctcaa tgggcaaagg gtcgccgcgg
     6181 cgccgctggc ttattcaggt ccaggaggtc tttttttcca gcctttcact aaaacaacaa
     6241 agccgatcac cccgatgatg agcgcgagtc cgcccagtaa cattaacgca tccatttatt
     6301 tgtctcctca cgtttgagca catccctctc ctcaaccttc ctggcaattg aggcgagaaa
     6361 tgtgcgacat gcaaaatcag gaaaagatca tttaagtcaa tgactgaaca aacacttagc
     6421 acagatatcg ccggtcaatt caggcctcgg gacttctgtt ttatttttcc tcagggaccg
     6481 ctgattcgaa atgactcccc atccgcactc aatcatgaaa ggctttcatt ttcgctaccc
     6541 aagaaattca aaggttcctt tgcctgagag gctttttcca tcagcatcct ttgtcaccaa
     6601 tcgaaaagat cctttaattt tggcaccgga caattttgta ccatcagcgg ggaaggcttc
     6661 caaggccaga gttccttctg tatcaaaccc aaaagaagta agtttcccgt caactatgct
     6721 ctcaattgaa cctcaaaatt ttcacccaac tccattggat cggctgcgac caattcatac
     6781 atgccggatt ccgtcccggt tgggatcctg aaggtcacca acggatcttt cgttccgcga
     6841 tcattggcga tcaaataata cccggaacgc acgccgtcaa ccgtattttg cgagcgtaga
     6901 taggtcgcga cgccgggtcc ggatatgtca gcattcacag tctcggagac ctttcctatg
     6961 aatcctgggt gctcaacccc ggcatcttcg gacaccgttt cagtcggtga ttcgtaacat
     7021 ccggcaacca aaaccagtag gagccagact tcaaaacata aaattttgtg aaatgatgtc
     7081 ataatggaat ccaattcgtt atatgagaaa tggatagatg aacagttacc tctttatggc
     7141 catgggcacc tgcgcaagaa cccgacgagg acgggccacg agggtttgtg cgtacttgct
     7201 actagttaac caatagcaaa agagaatggg aagagggtaa atcctgacaa aatatttgtc
     7261 atgcacgatc aaacgtccaa ttgacgcaga ggaaaagaca aaaaattcat ttcataaaaa
     7321 aacagcgcat tcctccccct tcaacttgat gaggggggag gaatgcgcaa atgccacaag
     7381 aaaacacatc ttaaaagacg ctccccaggc tgctgtcagg agcttcactc agaaccatta
     7441 agctttcgat cttccagtta cgcatggtcg ggatggtgaa tggacgccac cgaccattga
     7501 ccgccgtgaa cgaccttgtc
//
LOCUS       DATIAL010000425         3419 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_937749, whole genome shotgun sequence.
ACCESSION   DATIAL010000425 DATIAL010000000
VERSION     DATIAL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937749"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VLA60_13220"
     CDS             <1..1070
                     /locus_tag="VLA60_13220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10369.1"
                     /translation="LKSGTGMTHFAILMGARGSESMMMIPQDEDSCDPPALPGDSFPE
                     TLTPTIIGDSEPIQQLVEMVRLIAASEATVLIQGESGTGKELVAKTIHALSRRRLRPF
                     IVVECSALPETLLESELFGHVRGSFTGAVADRKGLFEEAEGGTIFLDEITDTTPAFQA
                     RLLRVLQEGEIKPVGSNGSIKVNVRVISAGNKPLEHLVATKLFRADLYYRLAVLPLTV
                     PPLRERREDIQLLVRYFLEQCARKHGREPMHLSPNAQTALVQHSWPGNVRELENLIER
                     VVVTCPHSPIEVKDFFADHSLQPDKSDLSSIGKIARQEAERSRILQALRDAKGDKTRA
                     ARALNISRSSLYNKLRDYHIS"
     gene            1534..>3419
                     /locus_tag="VLA60_13225"
     CDS             1534..>3419
                     /locus_tag="VLA60_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HSF10370.1"
                     /translation="MYPGKSRFLTASMFLALMGGASFWLTPSVVGAENASAPHVHQTG
                     MTHQESSDWAEQLKGQTRVEDALEGRAGLSEKIEVQHHRIMRRLEEQAQQDAQVQANS
                     GVFNNVSMMHQYMGQDGSSYLLAVDPDAALSTGAGAKCPAGVPTKHYDISMIDTEITL
                     NRWLDYYVGYMYVLTDNIDKVRAEEAQNKAARDKDGWDPGAVTTGLQGDYIQPLAIRA
                     NQGDCLKITLRNQMEIESGSLHVNGGSMVMSTTGKAASTSNPETVVEPGNSVEMEWYI
                     HPQTQEGVRRFHSYSNARELTVMGLFGALIVEPAGSKYLDPLGTGPAPELQSGWQAII
                     DNGSGHDFREYIIFYHEVGDEAFRPVNKKGDFLPQRDPLTDAYRPGGRALNYRSEPFG
                     IDEMHVQHEYFGFEDESLAYSAYTFGDAPTTIPRGYLGDPMKYRIVHGGSEVFHSHHP
                     HSGSIRWQRSPRADQQEHWYLGKDGPVKYPVVRTKSDRVDVEVIGPSEALDLEPECGS
                     GGCQHLAGDFLFHCHVAHHYVAGMWGYGRVYNTLQVGEVRTDIMADLRELPDRQGRVK
                     VGVSSDKLIGTTVDWFGKQFKIVDKSQKSNWKSNPAVVNIKDWVEMYLPAMGKPGHTD
                     DEKG"
BASE COUNT          861 a          801 c          954 g          803 t
ORIGIN      
        1 cgttgaaaag cgggacgggt atgacccatt ttgcgatatt gatgggggct cgggggagtg
       61 agagtatgat gatgattcca caggacgagg attcatgtga cccccctgca ttgcctgggg
      121 attccttccc agagaccctg actcccacga tcatcggaga cagtgaaccg attcaacaat
      181 tggtggaaat ggttcgattg atcgccgcga gtgaggcaac ggtgctcatt cagggggaaa
      241 gtggaacggg caaggaattg gtggcgaaga ccattcatgc cttgagtcgt cgacgcctcc
      301 ggccctttat agtggtggaa tgcagtgcat tacccgaaac cttgctggaa agcgaattat
      361 ttggccatgt acgtggatcc tttaccggag ccgtagctga tcgcaaaggc ctgtttgaag
      421 aagccgaagg cggcacgata tttttggatg aaattactga taccactcct gcattccaag
      481 cccgcttact tcgcgtcctg caggaaggcg aaatcaagcc cgtggggagc aatggatcca
      541 tcaaggtgaa tgtgagggtc atctctgcgg ggaataaacc tctggaacat cttgtggcca
      601 ccaagttgtt tcgtgcagat ttatattatc ggctcgccgt cctcccgtta accgtcccgc
      661 ccctgaggga gcggagagaa gatatccaat tgttagtgag gtattttttg gagcaatgcg
      721 ccagaaagca cgggcgtgag ccaatgcatc tcagccccaa tgcccaaacg gctctcgtgc
      781 aacattcgtg gcctgggaat gtccgggaat tagaaaactt gattgaacgg gtggtggtca
      841 catgcccgca ttccccaata gaagtgaaag acttttttgc cgatcattcg ttgcaacctg
      901 acaaaagcga tttgagttcg atcgggaaaa tcgctcgcca ggaagcggaa cggtcaagaa
      961 tcctccaagc cctccgggat gcgaagggag ataagacccg cgcagcgcgg gccttgaata
     1021 tcagtcgatc cagcctttac aacaaattgc gggactatca tatttcttaa actgctccct
     1081 tcccttccat tgtccctaaa atcctcattt accgctttta attgtccaat gttttagacg
     1141 ggcaactgct tgattgttca tcgaaatttt acgcaatttg aaaagcctgt ccaagatcct
     1201 ggaaattctc accactattc cgttgtctct tctcacctga ttgtgacctg aaattctctc
     1261 cagtattggt tgttgtgatt tggagcattg ataggtgttc tttggcactg ttgttgctca
     1321 tgaattcact cgccgacgct ggatagggtg tgatggaccg gcctccggaa tgcccgacct
     1381 cccacagagg gcgccttgcg tcggcacaaa aaaaatacgg ggccgggcta ttcattgaag
     1441 agacgaacat cttgaaatga ttgagccgag taagctgatg gagtgccaaa aatcagggtt
     1501 tttattcacc acataaattt aggaggccgg agaatgtatc caggcaaaag tagatttcta
     1561 accgcgtcaa tgtttttggc gttgatggga ggagcgagct tttggcttac cccgtctgtt
     1621 gtcggcgccg aaaatgcctc tgctccacac gttcatcaaa cgggtatgac tcatcaggaa
     1681 tcctccgatt gggctgagca actgaaagga caaacccgtg tggaggatgc attggaaggg
     1741 cgtgcaggcc tttctgaaaa aatcgaagtg caacatcatc gaatcatgcg gagacttgaa
     1801 gagcaggcac aacaggatgc gcaagtccaa gcgaactccg gggtgtttaa taatgtctcg
     1861 atgatgcatc agtacatggg acaggatgga tcaagctatt tgttagcggt ggatcctgat
     1921 gcggctctgt ccaccggggc gggagcaaaa tgcccggctg gcgtgcccac caaacactac
     1981 gatatctcca tgatcgatac ggagattacc ctcaatcgat ggctggatta ttatgtcggt
     2041 tacatgtatg tgttaacgga caacattgat aaagtccgcg ctgaagaggc ccaaaataaa
     2101 gccgcacgtg acaaagatgg ttgggatccc ggggcggtga cgacgggatt gcagggggat
     2161 tacatccagc ctcttgccat ccgggccaat caaggggatt gtttgaaaat cacccttcgg
     2221 aatcaaatgg agattgaaag tggaagtctt catgtgaacg gtggaagcat ggtgatgtcc
     2281 acgacgggaa aagcggcatc cacttccaat cccgagacgg ttgtggagcc gggaaatagt
     2341 gtggagatgg aatggtacat tcaccctcaa acacaagaag gagtgagaag atttcactcg
     2401 tacagcaatg cgcgtgaatt gacggtcatg ggcctttttg gtgccttgat tgttgaaccg
     2461 gccggatcga agtacttgga tccgttggga acagggcctg cgccagaact gcaatcagga
     2521 tggcaggcga tcatcgataa tggatccggt catgacttcc gcgaatacat cattttctat
     2581 cacgaagtcg gagacgaagc attccggccg gtgaacaaga aaggggactt tttgccacaa
     2641 cgcgatccat tgacggatgc ctatcgtcct ggcggtcgtg cgctcaatta tcgtagcgag
     2701 cctttcggta tcgatgaaat gcatgtacag cacgagtatt tcggattcga ggatgaatcg
     2761 ttggcttaca gcgcctatac cttcggcgat gcgccgacga ctattccccg ggggtacctt
     2821 ggtgatccca tgaaataccg aattgttcac gggggatccg aagtgtttca ttcgcaccat
     2881 ccgcacagtg ggtcgatccg gtggcaacga agcccgcgtg cagaccaaca agagcattgg
     2941 tatttgggaa aagacggccc ggtgaaatat cccgtcgtcc gcacaaaatc tgaccgtgtg
     3001 gatgtggaag tgatcggacc gtccgaagcc ttggatcttg aacctgaatg cgggtcgggg
     3061 ggatgccaac acttggcggg agacttttta ttccactgcc atgtcgccca ccattatgtg
     3121 gcggggatgt ggggatacgg tcgggtgtat aacaccctgc aggtcggtga ggtccggact
     3181 gacattatgg cggacttacg tgaattgcct gatcgtcaag ggcgggtcaa agtcggtgtc
     3241 agttccgaca aattaatcgg gaccaccgtc gattggttcg gtaagcaatt caagatcgtt
     3301 gataagagcc agaaatcaaa ttggaaatcc aatccagccg ttgtgaacat taaagactgg
     3361 gtggaaatgt acctgccggc catgggcaaa cccggccaca cggatgacga aaagggcca
//
LOCUS       DATIAL010000426         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1044753, whole genome shotgun sequence.
ACCESSION   DATIAL010000426 DATIAL010000000
VERSION     DATIAL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044753"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VLA60_13230"
     CDS             <1..209
                     /locus_tag="VLA60_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10371.1"
                     /translation="QRIRKPEALKQWYLSLIDELAQQGHVSTFSIKGLSWAEIDNLAD
                     IEQAERLVKIWEPVNASSVVSHHG"
     gene            complement(279..1085)
                     /locus_tag="VLA60_13235"
     CDS             complement(279..1085)
                     /locus_tag="VLA60_13235"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HSF10372.1"
                     /translation="MVPDHQTVTALVLAGTRNDQDPVARLVGVPNKILAQVGGEPMIS
                     RVLQTLEKSHTVGKRILCGPSWDTVQDHAFLKKCIESDEVHWIEPLQGPSVSVGRYLH
                     EHRQEFPILITTADHPLLTPEMVDYFLREARQTEADVTVGLVPYSLVAASYPQSKRTV
                     TRFTGGGYCGSNLFCLFTSKAERLVEFWSHIEQERKHPLRLIRTLGGMVLIRYVLGRL
                     SLSEALARLSRRLDLHVKEILLPFPEAAIDVDTPEDLALAEKILGARGKV"
     gene            complement(1113..2237)
                     /locus_tag="VLA60_13240"
     CDS             complement(1113..2237)
                     /locus_tag="VLA60_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10373.1"
                     /translation="MIRVEPVNDRATLKEFIRLPWKLYRSDPFWIPPLLVERLHTFSP
                     KNPYFQHAQWQCWIAYQGNEPVGRISAQIDDFHLRRYKDQTGFFGLLEGEPAPKVFQA
                     LFHTAEQWLQTHGMKSVRGPFNLSINQECGLLVEGFDSPPMIMMGHSRPYYSQLVEQN
                     DYVKAQDLLAYRIGIHFVFPPGVSLFLKKAKTSMRIRPLRRKYLKEDLAIIKDIYEEA
                     WSENWGYLPFSDAEFSQIGQQLKLLVEDDFVQIAEIDGEPTAMLVAFPNVHEVIRDLN
                     GRLFPWGWLKILWRLKASYPKSGRVALMGVRKRFQSSPMGAAMAYGLIEAVREAGLRK
                     HIQQIETSWILEDNNRMRHILESLGAEQYKTYRIYQKTLQ"
BASE COUNT          655 a          611 c          523 g          612 t
ORIGIN      
        1 atcaacgcat tcgaaagcct gaagccctaa agcaatggta tctatcactt attgatgaat
       61 tggcccaaca aggccacgtt tccacctttt ccatcaaagg attgagttgg gctgaaatcg
      121 acaacctcgc agatatagaa caggctgaac gcctggtgaa gatttgggaa cctgtcaatg
      181 cctcttctgt cgtttctcac catggctgat atctcagatt attactttta accaatccta
      241 aaataaccgc ttaccctctt cctgaggatc ccggactctt atacttttcc tcgagccccc
      301 aaaatttttt ccgctaatgc gagatcttca ggggtatcca catcaatggc agcttcagga
      361 aagggaagaa gaatttcttt cacatgaaga tcgagtcgac gactcagccg ggccaatgcc
      421 tctgaaagag ataaccggcc caacacatag cggatcaaaa ccattccccc aagtgttcga
      481 atcaaacgaa gaggatgctt acgctcttgt tcaatgtgac tccaaaactc caccagccgc
      541 tcggccttcg atgtaaataa acagaataaa ttgctcccac aatagcctcc acccgtaaac
      601 cgagtgaccg ttcgtttaga ttgaggatat gatgccgcga ccaatgaata cggtacaagt
      661 cccactgtga catccgcctc cgtttgtcgg gcttctcgaa gaaaataatc caccatctcc
      721 ggggtcaaca agggatgatc ggccgtcgta atcaaaatag gaaattcctg gcgatgctcg
      781 tgcaaatatc tgcccacact gacgcttggg ccttgtagag gttcaatcca atggacttcg
      841 tctgattcaa tgcacttttt aagaaaggca tggtcttgaa ccgtatccca tgaaggcccg
      901 cataatatcc gctttcccac cgtatgagat ttttctaacg tctgcaatac tcgggatatc
      961 atgggttcgc cacccacttg tgcgagtatc ttattcggta ccccgaccag tcgggcaacc
     1021 ggatcctgat cgttccgggt tccagccaac accagagccg tgacggtttg gtggtcaggt
     1081 accataggta ttcatgaata aaatgccagt gatcattgaa gagttttctg atagattcga
     1141 taggtcttat actgctcagc tccgagagat tctagaatat gacgcattcg attattatct
     1201 tccaatatcc aggacgtttc gatctgctga atgtgttttc gaagtcccgc ctctctcacc
     1261 gcctcaatca aaccgtaagc catcgccgca cccatcggac tggactggaa acgtttacgg
     1321 actcccatta aggcgacgcg accggatttg ggatacgaag ctttcagccg ccataggatt
     1381 ttaagccacc cccatgggaa aaggcgtccg tttaaatctc gtataacttc atgcacatta
     1441 ggaaaggcga ctaacatggc ggttggctca ccgtctattt cagcaatttg cacaaaatca
     1501 tcttctacca atagtttgag ctgttgcccg atctgggaaa attcggcatc gctgaaagga
     1561 agatatcccc aattttctga ccatgcttct tcataaatat ctttgataat tgccaaatcc
     1621 tccttcagat actttcgccg taagggacga atgcgcatag aagttttcgc ttttttgaga
     1681 aacagagaaa cacctggagg aaaaacaaaa tgtattccaa tccggtaggc aagaagatct
     1741 tgggccttta catagtcatt ttgttcaacc aactgcgaat agtaaggtct cgagtgtccc
     1801 atcataatca tgggcggaga atcaaatcct tcaactaaca ggccacattc ctgattgatg
     1861 gataaattaa aaggcccccg aacactcttc ataccatggg tctggagcca ctgttccgca
     1921 gtatgaaata gagcttgaaa aaccttgggg gcaggctctc cctcgagcaa gccaaaaaat
     1981 cctgtctgat ccttataccg acgtaaatgg aaatcatcga tttgcgcact gatccggcca
     2041 actggttcgt tcccttgata ggcaatccag cattgccatt gggcatgttg gaaataggga
     2101 ttctttggag aaaaggtatg gagccgttcg acgagtaaag gaggaatcca aaaagggtct
     2161 gagcggtaca gcttccaggg aaggcgtata aattccttga gagtcgcccg gtcattgacc
     2221 ggttcaaccc gaatcaaaat gcttttccct gctcatcctc acccacccac tgagtacagg
     2281 gaggcggact agtaacacac atcaggagtc accgttctcg gcagcccttt agttatcatt
     2341 gcttttgttc ggttaactga gccacccagg gatagtggga acgttcctgc ccggaatagc
     2401 c
//
LOCUS       DATIAL010000427         6766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_185589, whole genome shotgun sequence.
ACCESSION   DATIAL010000427 DATIAL010000000
VERSION     DATIAL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185589"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(701..1585)
                     /locus_tag="VLA60_13245"
     CDS             complement(701..1585)
                     /locus_tag="VLA60_13245"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSF10374.1"
                     /translation="MIQISYISSATRSMSQDDLEDILRVSRENNAQSGITGMLLYGNK
                     TFIQIMEGEEKVVNALIEKIKRDPRHTDFHVLKKKTIERREYSDWSMGFKRVSEDDFQ
                     AVKGLETLSEKDFNTAFLGSHGSIVESLMDHFRKDRIRKFGQEELSLDQEDRLIQILH
                     QTIRAAVRVLAVLMVFTILWGVVDVVYIIYSKLISPSFTTFTIRDIVSTFGAFLAVLI
                     AIEIFINITLYIRKDVIHIKLVVATALMAIARKVIIFDFKEMTPPYIFGTAAVVLALG
                     ITYWLIERRLTWSEPDNL"
     gene            complement(1994..2950)
                     /locus_tag="VLA60_13250"
     CDS             complement(1994..2950)
                     /locus_tag="VLA60_13250"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HSF10375.1"
                     /translation="MKHPTPQRPMTSILLVFGILSIGAIFAMVDIPLAVSQPVSIPIA
                     SITGDIAMDAANPIWESVPGVVVPLSGQTITTPMHPNISVKTVMVKMATNGKDIGVWA
                     NWSDQTINNTTIGPQDFRDQMAIQFPVQDAGAPPFQCMGQSGGTVNIWRWNAEWQKDL
                     GMGVAGMWDVDQQYPSIAWDYYYEEPSRGVTYTNRTGRSSGPFNEGIWSGNIMSDPSL
                     RISSVEDLNANGFSTLTTQATQNVLGNGLWEPYGALKGGCCNGPTWRVVMKRSLTTDD
                     PSDVQFKPGSSFPVAFAVWDGSNVERNGMKGISTWFTAQMPN"
     gene            complement(3041..3679)
                     /locus_tag="VLA60_13255"
     CDS             complement(3041..3679)
                     /locus_tag="VLA60_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HSF10376.1"
                     /translation="MNQITPKFSDTDIHILECGLGWIKDAEDAFADKLFHRLLRDHPE
                     VTSSLHSMGLQPFSRRIVQILDTIIRDIRSCGKIHSPIREQWTDLLPTTVSHLEPSQL
                     LRMAETYLDIFSELAEDAWSPAMESAWRKIIHEVSVSLWGQQPESLSLAKIASPFPFI
                     KRRNYCMNQPFTFLLGTIMILAGSIASIGLWSRDRLAEAKLSRNLRLKKVWC"
     gene            complement(3907..5154)
                     /locus_tag="VLA60_13260"
     CDS             complement(3907..5154)
                     /locus_tag="VLA60_13260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF10377.1"
                     /translation="MNADVLIIGAGLAGLSCATTLADHGIDFLLLEASEEVGGRIGSD
                     HLDGFVLDRGFQVFSTAYPEAKRLLDYPSLELHHFFPGAVIRCLGKWHRMPDPFRHPL
                     QSIQALFTPIGTLSDKIRVATFRHLARSGNLNDLFQRPETTTFQHLKNFGFSSSMIER
                     FFRPFLGGVFLDPELQTSSRMAEFVLRMFGSGDISLPAKGMGAIPRQLASGLPPGRIR
                     TQARVTSIQKTSVTLESGEALSARAIVLATDGPAATKLVPGLKSSPYRRVTCLYYSAP
                     EPPYSGPFLILNGEGAGPINNLCVLTQIAPTYSTTKSQLISVSVLDRADFSKKDLAIR
                     VRQQLREWFGSSVEDWQPIRTYHITSAQPVQVPPFPNPFSRQAHVQDGIFVCGDFCAT
                     GTIDGALFSGRRAAEDIRKWLET"
     gene            complement(5227..5583)
                     /locus_tag="VLA60_13265"
     CDS             complement(5227..5583)
                     /locus_tag="VLA60_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF10378.1"
                     /translation="MAAKGQRTLTKKQKLPSRPPKLDTIHHVAIPVSNVAEAVDWYTS
                     RFNCCVDYIDDTWALLAFANTKLALVLPREHPPHFALSRPDAHKFGELVTHRDGLNSV
                     YITDAFGNSIEVLEES"
     gene            complement(5634..6143)
                     /locus_tag="VLA60_13270"
     CDS             complement(5634..6143)
                     /locus_tag="VLA60_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HSF10379.1"
                     /translation="MSEVKKAILLVGHGGIPKGYPADLITKLKRLEAQRRATGSPMSP
                     EELELDTKVRTWPRTPETDPYQAGLESLGAAMKPLLNGSMFGLAYNEFCGPTLAEAVE
                     SLIHQGAQTITVVSTMFTPGGSHSESEIPEELEELRKKHPKVTLIYAWPFNLEKVSKM
                     LIEHIQQFS"
     gene            complement(6156..>6766)
                     /locus_tag="VLA60_13275"
     CDS             complement(6156..>6766)
                     /locus_tag="VLA60_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HSF10380.1"
                     /translation="MLEKWINHMGLEKALDSIIFPVLRLIGDLWHQGGMSLRGEQSVS
                     RLVRQHLINVLREEPPLGGPHALIACVPGDFHEIAPLTAAVLLRGLGWHSIYLGPNVA
                     FEMLEIALRRKQTQLILLSCNLEPEEKILRSWLQKITRLLQPLCAVMVGGPGFKPFVP
                     LLRSHNITYFTQVHDLKTLQQRTGMAESARAFHPLSFISWQP"
BASE COUNT         1696 a         1573 c         1767 g         1730 t
ORIGIN      
        1 agtctgggtg gtcgggggta attccaaaca gactcttgcc ctcgcttcgg aatctggatt
       61 tccggatttt gttcaaagat gagtttttgc attgtattgt cctaaggtat atctcttttg
      121 aaaagacggc taatgcccca acccctcatg ttaacatttt ctagagcagt cctttctttt
      181 atttaactac atcacccttg aactctccaa ggagaacctc caaaacccta gacaattctg
      241 caagactcca tttgttgacg aagagtctct gacgaatgga ataaccggtc gcctctaaaa
      301 cgtgatggtg ggtgaatcag gacctgccgg ccatggataa ccggaagttt gtgaaaaaca
      361 tgtttacgga gaaagccatg cagactcccg tacaatggga tttgacttgg ggaaaactgc
      421 caaagatacc tgaatggtat cgagaggtgg gtgtgacaag atgcgccgtt cgccggaggt
      481 caggggaaaa tagttggtcg agggtcaact ggttgtaaag gttgaagaat ctgcaataca
      541 gtaatatcca atgatggtgt aagctgtttg ctatttcttg cgagaatctc gtaccgggaa
      601 ccacgtagcg gtgagaatta caaattcttc agcggatatg acatgagaca cgactggtct
      661 cccgtcaatg aaaatggaca gacaattgca tcgttaatca ttacagatta tccggctcac
      721 tccaggtgag ccgtctttcg atgagccagt aggtgatacc caaagccaat acgaccgcag
      781 cagttccaaa aatatagggc ggggtcattt ctttgaaatc aaatatgatc acctttctgg
      841 caatggccat taatgcggtc gcgacgacta gtttaatgtg tatgacatct ttgcgaatat
      901 agagagtaat gttaatgaaa atttctatgg caatgagtac ggctaaaaag gctccaaatg
      961 tgctgacgat atcccgaatg gtgaatgtgg tgaaacttgg agagatcagt tttgaataga
     1021 tgatatagac gacatcaacc actccccaga gaatcgtaaa gaccatcagc acggctaata
     1081 ctctgactgc ggcccgaatg gtctgatgaa gaatttgaat gaggcgatct tcctgatcaa
     1141 gggacaattc ttcctgcccg aattttctga ttcggtcctt acggaaatgg tccatcaagg
     1201 attccacaat gctcccgtgg cttcccaaaa atgccgtatt gaagtctttt tccgataaag
     1261 tttcgagtcc cttgacggct tgaaaatcgt cttcggaaac ccgtttaaac cccatgctcc
     1321 aatcagaata ttcccggcgt tcgatggttt ttttcttgag cacgtgaaag tcggtatggc
     1381 gtgggtcccg tttgattttc tcaatcaacg catttaccac tttttcttcg ccctccatga
     1441 tttggataaa ggttttgttt ccatatagca gcatccccgt aatcccggat tgggcattgt
     1501 tttcgcggct aaccctcaga atatcctcca agtcatcctg agacatggat ctcgtggccg
     1561 aactgatata agaaatttgg atcatcggtt gaatccccct catctgggtt gaaaggctat
     1621 gatgtcgttt ctagattgta accgcagagg aatgagtccg gtaggtcata tggcacagaa
     1681 ggaggacagg gatcggggaa gtttccaact caagcgctag gagtatagac tccgatttta
     1741 gtaggaggat ccgtttctta aggcgcggaa atatttctcc tcaaaattag tgaaaactcc
     1801 acacttggaa agtcgggatt gagagcaagg ctacgtatct tcgtttcttg gcacctgatg
     1861 ggcacagtgt gaggtattga actgacacgc cctcgaaggg cgtgtcacca ctaagttttc
     1921 cagaagtcta tccgcctaaa ccccaggttt tggtgagatt ttttggtact ggagggtttc
     1981 cttccgattt ctgctaattc ggcatttgag cggtaaacca ggtcgaaatc cctttcatcc
     2041 cattacgctc tacattcgag ccgtcccaca cggcaaaggc cactggaaag cttgaaccgg
     2101 gtttaaactg gacatcactg ggatcatcag tcgtcaagga tcgtttcatg acaacccgcc
     2161 acgttggtcc attgcagcac cctcccttga gggcaccata cggttcccat aacccgttgc
     2221 ccagcacgtt ttgtgtcgcc tgggtggtga gagtgctaaa gccattggcg tttaggtctt
     2281 ctactgaact gatccgaagt gaagggtctg acatgatgtt tcctgaccag atgccctcat
     2341 taaacggccc ggagcttcgt ccggttcgat ttgtataggt gacgccccgt gagggttcct
     2401 catagtagta gtcccaggca attgaggggt actgttgatc cacgtcccac atgccggcca
     2461 ctcccatgcc aaggtctttt tgccattcag cgttccatcg ccaaatattg acggttcctc
     2521 ccgattgacc catgcattga aatggaggtg ctccggcatc ctgaaccgga aactggatag
     2581 ccatttgatc ccgaaaatct tgaggtccaa tggtggtgtt gttgatggtc tggtcactcc
     2641 agttcgccca gacaccaatg tccttcccgt tggtcgccat cttcaccatg acagtcttca
     2701 ccgagatgtt aggatgcatg ggagtggtaa tggtctggcc gcttaatggc accaccactc
     2761 cggggacgga ttcccaaatt ggattggccg catccatggc gatgtctcct gtgatggaag
     2821 caatgggaat ggagaccggt tggctcaccg ccagggggat gtcgaccatg gcgaagatag
     2881 cccctatcga cagaattcca aaaacgagca ggattgaagt cattgggcgt tgaggcgttg
     2941 gatgtttcat atttctcctt tacgctgaag gatcatttct caatattttc gattcttgga
     3001 acatatcgtt ttggaacctt gttctcacac cggtgacgaa tcagcaccat acttttttaa
     3061 gcctgaggtt ccttgatagc ttggcttctg caagccgatc acgactccac agcccgatgg
     3121 aggcgatgct gcccgccaaa atcatgatgg tccccaataa aaatgtaaat ggttggttca
     3181 tgcaataatt cctccttttg atgaatggaa agggcgatgc gatctttgca agagacaagg
     3241 attcaggttg ttgtccccac aagctgacgc tcacctcgtg aatgattttt ctccaggcgg
     3301 attccatggc ggggctccag gcatcttccg ccaactcaga aaaaatgtcc aaatacgtct
     3361 ccgccatcct gagcagttga ctcggttcca ggtggctcac cgttgtggga agcaggtctg
     3421 tccattgttc tctgatgggg gaatgaattt ttccgcatga gcggatgtcc ctgataatcg
     3481 tatcgaggat ctggacgatt cgcctgctga atggttgtag tcccattgaa tggagagagc
     3541 ttgtgacctc aggatgatct cgcagcaggc gatgaaaaag cttatcggca aaagcgtcct
     3601 cagcgtcctt aatccatcct agaccacatt ccaggatatg aatgtccgta tctgaaaatt
     3661 tgggggtaat ttgattcatg tcaaggtgcc tttccttaag gtctctgaat gttgctcctc
     3721 taacagtgta caccctggag tttgagggag gctaaaagga ggccgaaggg aaatggaaaa
     3781 accttagaca aacggaccgc tcaatctctg tgtgcgtgtc tgaaggcaga cagtctcaat
     3841 gacaggaatc gaaatcgaaa tgttgtcggg tagggcaatt ctagaaaaca ggaaggatgc
     3901 cggatttcat gtctcgagcc atttgcgaat atcctccgcg gcacggcgac cggaaaagag
     3961 ggctccatca atagtcccgg tggcacaaaa atccccacac acgaagatgc catcctgaac
     4021 atgtgcctga cgggagaacg gattaggaaa tggtgggacc tggacgggtt gcgcggatgt
     4081 gatgtgatag gttcgaattg gctgccagtc ttcgactgat gagccaaacc attcacgcag
     4141 ttgttggcga accctgatgg ctaggtcttt tttggaaaaa tcagcacggt ctagcactga
     4201 aacggagatc aactggcttt tggtcgtgga ataggtcggg gcgatttgag taaggacaca
     4261 gagattattg attggaccgg ctccctctcc gttcaatatt agaaatggtc ctgaataggg
     4321 cggctcaggg gcgctatagt agagacaggt cacgcggcga tagggagatg atttgaggcc
     4381 gggaacaagt ttcgttgcgg ctggtccgtc ggtggccagg acaatggcac gagcggatag
     4441 ggcttccccg gattccaagg tcaccgatgt cttctgaatg gatgtgaccc tcgcttgagt
     4501 tcgaattcgt cctggcggaa gaccggatgc cagttgtcgg ggaatggctc ccatcccctt
     4561 ggcgggaagt gaaatgtctc cagagccaaa catccgcaag acaaattccg ccatgcgact
     4621 ggaagtttgt aattccggat ccaaaaatac accaccaaga aatggtcgaa aaaatcgttc
     4681 gatcatggag gaagaaaatc cgaaattttt gaggtgttgg aatgttgtgg tttcaggcct
     4741 ttgaaataga tcgttcagat ttccggaccg ggccaaatgg cggaaggtgg ccacccgaat
     4801 tttatctgaa agggtaccaa taggagtgaa aagcgcctga atgctctgca agggatggcg
     4861 aaagggatcc ggcattcgat gccatttccc aagacaccga atcaccgcac cagggaaaaa
     4921 atgatgaagc tcgagggaag gataatccag caggcgtttg gcctcaggat aagccgtgga
     4981 aaagacctgg aagccccggt ctagaacgaa gccatcgagg tgatctgatc caattcgccc
     5041 tcctacctct tcggatgctt ccagaagtaa aaaatcgatc ccatgatccg ctaacgttgt
     5101 tgcgcaggaa agtccggcaa ggccggctcc gataatgagc acatcagcat tcataataaa
     5161 agatttgtgt ttgtttaggc ccaggaagaa agaaggatat gcctgctgtg tcgaaagatg
     5221 ttcaacttat gactcttcta acacctcaat ggaattgccg aaggcatcgg tgatgtagac
     5281 cgaattgagt ccgtcacgat gggtaacaag ctctccaaat ttgtgggcat ctggacgact
     5341 gagcgcaaaa tgaggcggat gttcccgagg gaggaccagg gcaagcttgg tattggcaaa
     5401 ggccagaagc gcccaggtgt catcgatata atcgacgcag cagttgaagc gggaggtata
     5461 ccaatcgacc gcttcggcca cattcgagac gggaattgcc acatgatgga tcgtatccag
     5521 ttttgggggt ctggacggga gcttttgctt tttagtcaag gtgcgttgcc ctttggctgc
     5581 catgcgcttt tgcttacctc tctttgcgtg tgcttcatga agggtgaaag aacctatgaa
     5641 aattgttgga tgtgctctat cagcattttt gagaccttct ccaggttgaa cggccaggca
     5701 taaatgaggg tgaccttcgg gtgtttcttg cgaagttctt ccagttcttc ggggatttca
     5761 ctttcggaat gcgatcctcc cggagtaaac atggtcgaga ccacggtaat cgtctgtgct
     5821 ccctggtgaa tgagggattc aaccgcttcg gcgagtgtcg gtccgcagaa ctcattatac
     5881 gccaagccaa acatcgatcc attcaagagt ggcttcatgg cggccccaag ggattcgagt
     5941 ccagcctggt aaggatccgt ttcaggagtc cggggccatg tgcgaacctt ggtgtccaat
     6001 tctaattctt cgggcgacat gggactgccc gttgcccttc gctgggcttc aaggcgtttc
     6061 agttttgtaa tgagatcagc cgggtatcct ttgggaatgc ctccgtgtcc gacaagaaga
     6121 atggcttttt tgacttctga catgttgcgg gctccttatg gttgccagga aatgaatgat
     6181 aatgggtgga aagccctggc gctttcagcc atgccagttc gttgttgcaa cgttttgaga
     6241 tcatggacct gggtgaaata tgtaatattg tgagagcgaa gaagaggcac aaacggtttg
     6301 aaccccggtc cgcccaccat taccgcacac aatggttgaa gaagtcgagt gatcttttgc
     6361 agccaggacc gtagaatttt ctcttctggt tctaggttgc aggaaaggag aatgagctgt
     6421 gtttgtttcc ggcgcagggc aatttccagc atctcaaatg caacgttggg cccgagatag
     6481 atactgtgcc atccaagtcc tcgcagaaga accgccgcag tcagtggagc aatttcatga
     6541 aagtccccgg gcacacaggc gattaaggcg tgcggtcctc ccagaggagg ttcctcgcgt
     6601 agaacgttaa tgaggtgttg acgcaccaat cggctcacgc tttgttcccc cctcagactc
     6661 atccctcctt gatgccacag gtcgccaatc agtcgcaaga caggaaaaat aatgctatcc
     6721 agggcttttt caagccccat atgattgatc cacttttcaa gcatga
//
LOCUS       DATIAL010000428         5338 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_401820, whole genome shotgun sequence.
ACCESSION   DATIAL010000428 DATIAL010000000
VERSION     DATIAL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401820"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="VLA60_13280"
     CDS             complement(<1..1862)
                     /locus_tag="VLA60_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF10381.1"
                     /translation="MLVTKRQFLKITAGTVAAVALADNALALTALQPVVEVGNPLGEY
                     PDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDMY
                     GNRGTFAHNPRMCLKGYTYHRRVYGPYRLKGPLMRRGWKAWMDDGSPELDPTVMTKYK
                     FNARYLDDMLRVSWDTAFTYLAKAMIVIANRYSGEAGARRLREQGYPPEMIEMMKGSG
                     VRSMKFRAGMPVLGVIGKMGITRMNGGCGALLDSWVRKVGPDNAQGGRYFNNLTWHGD
                     QDPSQPFWSGAQAIDCDLSDMRFSKMNTSWGKNFVENKMPEAHWKLESIERGGRVVVI
                     TPEYNPTAQRADYWMPVRPETDGALFLGACKIILDDGLQDNDFIRSSTDFPLLIRTDT
                     LQYLDPRDVIKDYAFPDFSKSYSGKVQGLSPSQIARLGGFMVWDLNQDKAVPLHRELV
                     GWHFKNSGLDPALTGSFRVKLLSGREADVMPLFQMYQVHLQDYDLDTVHQINRCPKDL
                     IVRWARDNGTIKPAAIHNGEGVCHYFHMTSMGRAAAMVMTLTGNMGKFGTGCHTWSGN
                     YKVGVWAATPWSGEGIGIHTGENPFKITTDPNAHGKEINYRPYYYGEETTYWNHG"
     gene            complement(1915..2103)
                     /locus_tag="VLA60_13285"
     CDS             complement(1915..2103)
                     /locus_tag="VLA60_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10382.1"
                     /translation="MMEFWVANPLIEMIFGIVSFVGVLVLGWANLMVEPPKTAVAIPA
                     ELNVWERHRLRQSQKKEE"
     gene            complement(2244..4256)
                     /locus_tag="VLA60_13290"
     CDS             complement(2244..4256)
                     /locus_tag="VLA60_13290"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HSF10383.1"
                     /translation="MSPIIWGEEMNGMRGGMKLKKNAAMMLISIIVGMLLLPIGVAMP
                     ILFGNGEAIASGGGQSPQPAQEVVEGEEGEAPVEMGRDIYYKTEGVVSSETAPVTADN
                     THDYPRYGNFESRVLIWVANQQHLYYGSFVLAVPIFCMVIEFIGMVTKDKAMAKKYDQ
                     LAYDFIRISLTAYSLTAILGGILLFTFLTLYPTFFSYLSGIFRPVMHIYALLFVAESG
                     TLYIYYYGWDRMREGVLKWVHLSIAVVLNVIGTVLMFLANSWIAFMMSPAGVDEQGRY
                     LGNIWHAIHSALWNPLNVHRILGNMAFGGSVVAAYAAYRFLSAKSDEERAHYDWMGYV
                     AMFIAVIFLIPLPFAGYWLMREVYAYRQQMGITLMGGLLAWLFIIQATMIGALFMTTS
                     YYLWQCFGRMPGAERFQKYIKYLVFIAVVGLLVFITPHTIVMTPAELKAMGGQQHPVL
                     GNYGVMSAKNGGINMLIIVTIVSFIWYQRGNLIPAVKWKKLGNIFMTCFIVMGSLNII
                     WLAVYGYYIPANVRIGLSVPQVATTLSCLLLMMPLNLAMLKNAKMAGPIQWGKMPARS
                     QYALIALATEFTWMMALMGYIRSSVRLFWHVNEVMRDNSPWAYTHTIGFAANMISFNV
                     LLFWSTILFVFWLAAFAGKKSPVPETPKVPESQAVRQFTPQPASRS"
     gene            complement(4684..5277)
                     /locus_tag="VLA60_13295"
     CDS             complement(4684..5277)
                     /locus_tag="VLA60_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HSF10384.1"
                     /translation="MKFYLDTVSLTEIQEIGRLGILDGIAMNATLVTEQGLNFYQGIR
                     EICRYVDSPISVGVLSIEEEAIVKESKELSKIHKNVLVKCPLTPAGLKATKRLTAEGI
                     RVNVSLCFSLTQALIAAKAGAWCVSICLDRSGDDQSKGDDIIRNIVTVFQNYGLSTQV
                     LLAGIRSPHDVLEAALAGGHICTMRFPMFQQLFDPAV"
BASE COUNT         1398 a         1516 c         1166 g         1258 t
ORIGIN      
        1 ccgtggttcc agtacgtcgt ctcttcgccg taatagtatg gacggtaatt aatttctttc
       61 ccatgggcat tcggatccgt cgtgattttg aacggatttt ccccggtgtg gatcccgatg
      121 ccttcccccg accatggcgt ggccgcccaa acaccaacct tatagtttcc tgaccatgta
      181 tgacatcccg taccgaattt ccccatgttt ccggtcaggg tcatcaccat ggcggccgca
      241 cgccccatcg acgtcatatg aaaataatgg cagacgcctt ctccattatg aatggcggcc
      301 ggtttgatgg tcccgttatc tcgagcccat cgcacaataa ggtctttcgg acatcgattg
      361 atctggtgga cagtatccag atcgtaatcc tgaagatgga cctgatacat ctggaaaagg
      421 ggcatgacat ctgcttccct cccactcagc aatttcaccc tgaacgatcc cgtcaacgcc
      481 ggatcgagac cgctgttctt aaagtgccaa cccaccagtt ctcgatgaag cgggaccgcc
      541 ttgtcctgat tgagatccca caccataaac cctccaagac gagcgatctg cgaaggcgaa
      601 agcccttgca ctttgccgga gtaagatttg gagaaatccg ggaaggcata atctttaatg
      661 acatcacgcg gatccaaata ttgcagggta tccgtgcgaa tcaacaaggg gaaatccgtg
      721 ctggatcgaa tgaaatcgtt gtcctgaagc ccatcgtcta aaattatttt gcaggctccg
      781 aggaataacg ctccatctgt ctcggggcgg accggcatcc aataatcggc ccgttgtgcc
      841 gtgggattgt attccggggt aatgaccacc actcgaccac cccgttcaat ggactccagc
      901 ttccaatggg cttccggcat tttattttcg acgaaatttt tgccccagct tgtattcatc
      961 ttgctgaatc gcatatcaga aagatcacag tcgatggctt gcgccccaga ccagaatggt
     1021 tgcgaaggat cctgatcacc gtgccaggtc aaattattaa aataccgccc accctgggca
     1081 ttgtccggtc caacctttct cacccaacta tccaacagag ctccacatcc accgttcatc
     1141 cgggtaatgc ccatcttgcc tatgacacct aacacgggca taccggcacg gaatttcatg
     1201 ctccggactc ctgagccctt catcatctcg atcatttccg gaggataccc ttgttcccgt
     1261 aatcgcctcg caccggcctc gccgctatac cggttggcga tcacgatcat ggccttggcc
     1321 aaataggtaa acgcggtatc ccaggagacc cgcaacatat catccaaata gcgcgcattg
     1381 aatttatatt tcgtcatgac cgttgggtct aattcaggac ttccgtcatc catccaggct
     1441 ttccagccac gccgcatcaa tggccccttt aaacggtatg gaccatacac ccggcgatgg
     1501 taggtatacc ccttcaaaca catccgggga ttatgggcaa aggttccccg attcccgtac
     1561 atatcctcat aggtttgatg atcgtagttt tgctctacgc gcatcacgac cccgttccgg
     1621 acaaacgcac gaatacgaca ggcatgcgtg tcattcgggg aacaacacca ggtaaaggaa
     1681 ctgtcatacc ggtattgatc gtgatagacc ctctcccagg agcgatccgg gtattcaccc
     1741 aatggattgc ccacctccac aaccggttgc aatgccgtca acgccagcgc attatccgcc
     1801 aacgccacag ccgccacagt gcctgctgta atttttaaaa attgtctttt tgtgaccaac
     1861 atcaaacgct cctccataca aaggttgact gtttcatctc acgacacaaa gcctttattc
     1921 ctcctttttt tgagactgcc tcaaccgatg tctttcccag acattcaatt cggcaggtat
     1981 ggccaccgca gttttcgggg gctcgaccat aagatttgcc catcccaaca ctaagacacc
     2041 cacgaagctg actatcccga agatcatttc aatcagagga tttgcaaccc aaaattccat
     2101 catctcgctc tccttcacag ttcaaccact ccatcatgcg cttcaccttg aatgcgtgaa
     2161 cgcgcctttc agccccctgt cctcgcgccg acttcatgaa tccagaattc attcaccaac
     2221 ctatactttc cccatgaaac tcatcaggaa cgactggccg gttgaggtgt gaactggcgc
     2281 acagcctgtg actctggcac tttcggcgtt tccggtaccg gcgatttttt cccggcaaaa
     2341 gccgccagcc agaacacgaa tagaatggtc gaccagaaca gcagaacatt gaaggagatc
     2401 atattcgccg caaacccgat ggtatgggta taggcccatg gcgagttatc ccgcatcacc
     2461 tcgttcacat gccagaagag gcggactgaa gaacgaatgt atcccatcaa ggccatcatc
     2521 caggtaaact ccgttgccag agcaatcaga gcatattgcg accgcgccgg catttttccc
     2581 cattgaatgg gacccgccat cttggcattt ttcagcatcg ccagattcaa cggcatcatt
     2641 aacaggagac acgacaatgt agtggccacc tgaggaaccg acagcccaat ccggacattc
     2701 gcgggaatat aatatccata gactgccagc cagataatat tgagacttcc catcacgata
     2761 aaacaggtca taaaaatatt gcccaatttt ttccatttca ccgccggaat caaattgcct
     2821 cgctgatacc aaatgaaact cacaatcgtc acaatgatga gcatattgat cccgccattt
     2881 ttggcggaca tgaccccata gtttcctaag acgggatgct gttgccctcc catggctttc
     2941 aactcagccg gggtcatgac gatggtatgg ggcgtgataa acaccagtag gcccaccacc
     3001 gcaatgaaca ccagatattt aatatatttt tggaaccgtt ccgcgccggg catcctgcca
     3061 aagcattgcc ataagtaata actcgtcgtc atgaagagtg caccgatcat ggtggcctga
     3121 ataataaaca accaggcgag aaggcctccc atcagggtaa tccccatttg ttggcgatag
     3181 gcataaacct cccgcatgag ccaatagccc gcaaacggca agggaataag gaaaatgact
     3241 gcgataaaca tcgcgacgta tcccatccag tcgtaatggg cgcgctcttc atcggattta
     3301 gccgataaaa accggtaggc cgcataggcc gcaacgacgc tgcctccgaa tgccatattt
     3361 cctaaaattc gatgaacatt caacggattc cacaacgcgc tatgaatcgc atgccaaata
     3421 ttcccgaggt aacgtccttg ttcatccacc cctgccggcg acatcatgaa ggcaatccac
     3481 gaattcgcta aaaacatcag cacggttccg atgacattca agaccacggc gatactgaga
     3541 tgcacccatt tcaggactcc ttccctcatc ctgtcccatc cgtagtagta gatatatagc
     3601 gttccgcttt cggcgacgaa cagcaaagcg tagatgtgca tgacaggacg gaaaatccct
     3661 gagaggtagc tgaaaaatgt ggggtagagc gtgagaaagg tgaaaaggag aatgccccca
     3721 aggattgctg tcaacgaata ggccgtcaga ctgattcgga taaaatcata ggccagctgg
     3781 tcgtatttct tcgccatggc cttatccttt gtcaccatgc cgatgaactc aatcaccatg
     3841 cagaagatag gaaccgccaa gacaaaactg ccgtaatata aatgctgctg gttcgcaacc
     3901 cagatcagca ctcgactttc aaaattgcca tagcggggat aatcatgcgt attgtccgcg
     3961 gtgactggag cagtttcact cgacaccacg ccctcggtct tgtaataaat atcccgtccc
     4021 atttcgaccg gcgcttcgcc ttcctcaccc tcgacaacct cctgagcagg ctggggactt
     4081 tgccctcctc ccgaagcgat ggcttcgcca tttccaaata ggatcggcat tgctaccccg
     4141 atcggaagca gcagcattcc cacaatgatg ctgatcagca tcattgcagc atttttttta
     4201 agtttcattc ccccacgcat tccattcatt tcttcccccc aaataattgg actcatctaa
     4261 catccaaaaa aaacttctgc tgataaatat atctcccaag tttttctgac attgccctat
     4321 tcgtgatgcg accaatttca ttcccgtatt tcctgtatgt tgttgaacgc tttgcatggg
     4381 ccatgcccaa tcaaaataat tcaatgcccc cgcacaaagc cttgtaatcc taaagtttcc
     4441 aatgtttttt ctctggataa catccgcaga cggatcattg gcccacgaaa gaacgtggtg
     4501 ggtggtggtg gccacccacc gctttacgtg gctggactca ggaaaatacg gggggtacaa
     4561 aagggggaaa aactaggagc atgacgaaga acgataagat acctgcaaac ccgatcacgc
     4621 ttcggaaggg cgggagttct gaagagcaca atccggatcg gaggatgtgg ccagaaagga
     4681 caattacaca gcaggatcga aaagttgctg aaacatcggg aatcgcatcg tacaaatatg
     4741 ccctcccgct aatgctgcct caagcacatc atgaggagaa cggataccgg caagaagcac
     4801 ctgagtggag agaccatagt tttgaaagac ggtgacaatg tttcgaataa tatcgtctcc
     4861 cttggattga tcgtcgccgg agcgatcaag gcaaatggac acacaccagg caccggcttt
     4921 ggcggcaatc aaggcttgcg tgagggaaaa acacaaagac acattcaccc gtatgccttc
     4981 ggctgtcaac cgcttagtcg ccttgagacc ggcaggcgtc aaaggacact tcactaagac
     5041 atttttgtgg attttcgata attctttgct ttccttgacg atggcctctt cttcaatact
     5101 caacacgccg acactgatcg gactatccac ataacgacag atttctcgaa ttccctgata
     5161 aaaattcagc ccctgctcgg taaccaacgt ggcgttcatg gctattccat ccagaatccc
     5221 cagcctgcca atctcctgaa tttctgtgag gctcaccgtg tcgagataaa atttcatgaa
     5281 cgtttattac tcatgaacag gaatgacctt cctcggcgtt aaaagccctg ccgccctc
//
LOCUS       DATIAL010000429         8612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1044961, whole genome shotgun sequence.
ACCESSION   DATIAL010000429 DATIAL010000000
VERSION     DATIAL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044961"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VLA60_13300"
     CDS             <1..375
                     /locus_tag="VLA60_13300"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HSF10385.1"
                     /translation="QTQELPPGKKDVVRALQRVSFNKDDLNRYKSLGIVGENNEGGVT
                     LLEPEKVQPDDRAFVENLIKEENEDRLAIMSRIIETNETLAPSELPRVHKMFAALNRD
                     KALKGERIQLDNGTWTQKDATP"
     gene            348..8255
                     /locus_tag="VLA60_13305"
     CDS             348..8255
                     /locus_tag="VLA60_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HSF10386.1"
                     /translation="MDSKRRHTLTQESRLRDGHLSHPSQEAQESTSGRKERRSFQFHE
                     ALFGLIAFTGLIGFLCSSLPALAATPERLTTHPGLDYQPSVSKDGQTLAFVSTRSGNS
                     DIWVQTLKSAALTLPRQMTTHPASDQEPALNRDGTRLLYVSHKSDPRGDVYLLDLITR
                     EEQRLTDLTSGDGSPQWDQEEQGFFYLKSDPLQNTSAIYRKSFANQSEELVVAQATSF
                     SVNGQGQLLYSTGAHLTLLNLQDNSSTSFDQDSHALDLWPALDHNPSDLTTDRHLVFT
                     RYDLDTNDDGLVDTDDESSIWMRLWGPQQAELQKLYRITPAHTFHIYPAVSGDFLYYS
                     DLKAGDIFRMNIPAFLRDYVDLDHAKSLAASYQDHGQLDLALLVLGNISHNLLAQQPP
                     DARAEFDFSLAEAQTQEGNFLAARESLEPYTRQPGRTGALARIYTIVLRVQEQAQGIS
                     SAARRRLVTSGVSDLLTIGQEHRDMDEVYGQALIEAGRLYLFADDPLTALEYLVKVED
                     LHNKEIRAKGLFTRGEAYRILGDAPNVIRVFVDVIQLFGERSSWGKRAIQQVVMLSQQ
                     GKTERERITALNRIIPQHPDLPILIANARLTIADLYHEQGEQLSALETLDFVIPVPDL
                     PNELVIQAYRKKAAILSESERYQEAADTYAALSQFPGENQTELEGTKSLLILQLVKKA
                     LKDRKIGETRIAAKSLKQLIDQYPESVEAHRAYIETKVMLKDTAEVQDWYATLVKTHP
                     DHAVYQYGQALALSYAEPPDLPLVIRLLQRAMKKDPALGYVHQTLGWAYEQTERTSGN
                     KGYLEKAEQEYRIALELNDAGRFPDVESQLLLNLGNTYLALSNAREAYRHYRQREEQF
                     APSGETITELLYRKNYGEACFKAGRTEESLVQYQLTLRNVPPEQPGLQAEILERIGLS
                     HQDIGQYAKAIEAFSRALVLNRELGQEQNVTLLQRNIGVNLFNLSRASETGGREELKQ
                     ALGSYFTSLDHLTTGGGKTLSKGPGLFNVNVALNEGGSQAASGFDLRGEQKLMFSYIA
                     STYEQLDEPGPARDFYRKKLALLNQVKPATQDAAALTEKAIVLNRLGVLSHQLDEPEQ
                     AMESFRQSLNYTRTLNIPFGTSVNIYNLSTLAVESLLQGHTPDHNLVEAITSGIQDLQ
                     QQNYEDRNLFFTLTNTAMLLSLLPDPSPDPRLKPADAVQRMHEQFTTRTLPWSYYQKA
                     DSLLQKPALFSDSERLPAQFLVKLNQAELARTSDQPQVYRNIQDDLLKLVEDRQSPNS
                     WLWYLSQAEATADPLTHKKLLTQSVEAVLRFPAQTDPPTGQTNTWPAYERLIQLSVDQ
                     LIKEGRPDDAFTVAEQLSIRQMTSAVYEALGETVFLKGLGDYEPELHSLLGELRQARN
                     HGNVAAIEELAPALQDTLYALFEEYPWATASFWAYPPTRDLIFLGVDAQHPYLKILKG
                     RQGYHGFVHNGDTLHYSPLAVTEKGVTGDEEFHKRLKQSASAYVAMPQELESTLPSLT
                     LDQKPLTWVSNFYDFLNGYHQRSLFFSHITTPSTFLPSTPSSAGEIPFVLQQFSGVET
                     HDHPLASHTNIAAFLKNPDTFAFEVQEAQNVREFVSVLDFAGSQHHSILLFGGIPSSS
                     PSPWVLISSLLRAGFPHVIVSRTPLDSQTAARFLNQYLTHLNTLPPDEAVMAASKDLW
                     GSETGKYPFRHYGFAGMGPDERQEYAASIYDQEVAEAIHAFETKDFPASLLHIEHALA
                     LIEHAKKRQDFKELTTLAVETAFEISDYEKGLFFQQKLLDALTAETPANERAEAEYRL
                     GILHSRLEHFDLAVQHLEQANQIWEQGEQLDRLAEGMATLGIVRENMGAYTEALDQFN
                     QSFSLYQEIGEMGHTAFQYRRIGRIYYLRLGRYEKARENFLAALKLYQEQGDPQGEAE
                     ALYEVGLTYEKVGLFDQAAERYHQALSIAKELDDPKLLTTGDLYLANVAWFQGNYQTA
                     FQLLTQADKKAEQAKDPQLRIMVKNTRGLIYWTLNDTDKGLVHLHDAVTLSRSSNIQT
                     ELASSLNNLGLIYRQRGDHATALDFFQQAKTLDESLKSQWGLGYDHRNIGISLLALGQ
                     LEEAEGHFIKAEQISAEINNVINWVKALLELGNVNKALIRPEQALGYYERAHEISQRY
                     GIKEVEWRAAAGKATLLREEGKLPDALTWFTKGVEVVEGMRAALKIDELRNSFQTNKL
                     DLYRDIITLLITMNRTDDAFNFLERSRSRSFIDLLGNQKLAFKNQGDRETWTKINRLA
                     STVDSLKSELGSYEEPPADLQDRYRNTKALYEEAILEVKQQNPALSSFVSVDPLNLEG
                     VQQLLAPRVGLLTYFLTKDQLYLWLITKERTLFKTVSAKEEDLTRLVTRYRQLVQHLE
                     PVDDELQKLYGSLIQPVEPDIKNLEYLGIIPDGPLHFLSFAALKHGPAYLVDEIPLFY
                     APSASVFQFTFAKRQAVKNDKVLAIGNPDLGNFNYDLPLAELEAKSIKWNYPNMDILT
                     GTKATKEWVVKNISRYGIIHLAAHGEFDEGNPLLSSLWLASENPENRRLTVKEIFGLE
                     LNADLVTLSACQTGLGKLEAGELIGLNRAFVYAGTHALVSALWRVDDLSTSVLMKHFY
                     RNYVTANKARSLRQAQLLVKKDFPHPSYWAGFSLIGDYQ"
     gene            8296..>8612
                     /locus_tag="VLA60_13310"
     CDS             8296..>8612
                     /locus_tag="VLA60_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HSF10387.1"
                     /translation="MPDRIARVIFGIVFLTIVAWMFPSSNWAATLYAKQDDVKVNAEK
                     SPTSAVIATLALGDAVTVLAEEGRLAKVKTATGKIGWVFKFRLAEEKPSTGGGGLGLS
                     GLTG"
BASE COUNT         2159 a         2523 c         2101 g         1829 t
ORIGIN      
        1 caaacacagg agcttcctcc aggaaaaaaa gacgtcgtgc gtgccctcca acgagtgtcc
       61 ttcaacaaag atgatctcaa tcgctataaa tcattgggga ttgtcggtga aaacaatgaa
      121 ggtggtgtca ccctccttga gccggaaaaa gtccaaccgg acgatcgagc gtttgtggaa
      181 aatctgatca aagaagaaaa tgaggatcgt ctcgcaatta tgagccggat cattgagacc
      241 aacgagactc tggcaccctc agaattaccc cgggtccaca aaatgttcgc cgcactcaac
      301 cgggataagg cgctcaaggg agaacgaatc caactggaca atgggacatg gactcaaaaa
      361 gacgccacac cctaactcaa gaatccaggt tgcgagatgg acacctttct cacccttccc
      421 aggaagctca agagagcacc tcaggaagaa aagagagaag atcgtttcag tttcacgaag
      481 ccctattcgg cctgatagcc ttcaccggcc tgatcggatt cctgtgctcc tccctacccg
      541 ctctcgctgc gacacctgaa cgcctgacca ctcacccggg cctggattat caaccgtctg
      601 tttccaagga tggacaaacg cttgcattcg tttctactcg ttcaggaaat tcggatatct
      661 gggtccaaac cctcaagagt gccgccctca cacttcccag gcaaatgacc actcacccgg
      721 ccagcgatca ggaaccggcc ttgaaccggg atgggacccg actcttgtat gtgtcgcata
      781 agtccgatcc ccgtggcgat gtgtacctcc tggatctgat tacccgtgag gagcaacgat
      841 tgacggattt aaccagcggg gatgggtctc ctcagtggga tcaagaagaa caaggatttt
      901 tctatctcaa aagcgatccg cttcaaaaca cctccgccat ctaccggaaa tcttttgcaa
      961 accagtcgga agaattggtc gtggcccaag ccacgagctt ttctgtaaat ggtcagggac
     1021 agctcctgta ttccactggt gctcatttga ccttattgaa tcttcaggac aacagctcca
     1081 cctccttcga ccaggacagc catgctctgg atctctggcc tgccttggac cacaacccct
     1141 ctgacctcac cacggatcgt cacctggtgt ttacccggta tgacctggac accaacgatg
     1201 acgggctggt tgataccgat gatgaatcgt ccatttggat gagactctgg ggcccgcagc
     1261 aggcagaact ccaaaaactc tatcggatca ctccggccca cacatttcat atttaccctg
     1321 ccgtgtccgg ggattttctc tattactccg atctgaaagc cggagatatt ttccggatga
     1381 acatccccgc gtttctcagg gattacgtcg atctcgatca cgccaaatcg ctggccgcct
     1441 cctatcagga tcacgggcaa ctggatctcg cgctcctggt tctgggaaac atttctcaca
     1501 atctgttggc acaacaacct cctgacgcac gagcggaatt tgatttttcc ctcgccgaag
     1561 cacagacaca ggaaggaaat ttccttgcgg cccgcgaatc cttggagccc tacacccgcc
     1621 aaccgggacg gaccggcgcc ttggcccgga tatataccat cgtcttgcgg gtccaggagc
     1681 aggctcaggg catcagctct gcggcaagac gccggttagt caccagtggg gtgtccgatt
     1741 tactcaccat cgggcaggag caccgggaca tggatgaggt atacggtcag gctctgattg
     1801 aagccggacg gctctacctc tttgcggacg atcctctcac agccttggag tacctcgtca
     1861 aagtcgaaga tctgcacaac aaagagattc gagccaaggg tcttttcacc agaggagaag
     1921 cctatcgaat tttaggagac gcccccaatg tgatccgcgt gttcgtggat gtcatccaat
     1981 tatttggcga gcgttcctcc tggggcaaac gtgccatcca acaggtcgtg atgctgtctc
     2041 aacagggtaa aacggaacgt gagcgcatca cggccctaaa caggatcatc ccccagcatc
     2101 cggaccttcc catcttgatc gccaacgccc gcttaaccat cgccgatctg tatcacgaac
     2161 agggagaaca actgtccgct ctggaaactc tggatttcgt catcccggtt cccgacctcc
     2221 ccaacgaact cgtcatccag gcctatcgaa aaaaggccgc catcctttca gaatcggaac
     2281 ggtaccagga agcggcggat acctatgccg ccttgagtca atttcccgga gaaaatcaga
     2341 ctgaactcga aggaaccaaa agccttctca ttttacaatt ggtgaaaaaa gccctgaagg
     2401 accggaagat cggggagacc cgcatcgccg ccaaatccct gaaacagctt atcgaccaat
     2461 atcccgaatc cgtagaagcc catcgggcgt atattgaaac caaggtcatg ctcaaagaca
     2521 cggcggaggt ccaggactgg tacgccacct tagtcaagac ccatcccgat cacgccgtct
     2581 atcaatacgg ccaggccctg gcgttgtcct atgccgaacc gcccgacctc ccgttagtga
     2641 tccgcctcct ccaacgcgcc atgaaaaaag atcccgccct ggggtatgtc caccaaaccc
     2701 tgggatgggc ctacgaacaa accgaacgga cgtccggcaa caaaggctac ctcgagaagg
     2761 ccgagcagga gtatcgcatt gcgttggagc tcaacgatgc cggccgcttt cccgacgtgg
     2821 aatcgcaact actcttgaat ttaggcaata cctacctggc tctctcgaat gcccgcgaag
     2881 cctatcgaca ttaccggcag cgtgaggagc aattcgctcc gtccggcgag accatcaccg
     2941 aattgctcta tcgcaaaaat tatggagaag cctgttttaa ggcaggacgc accgaggaat
     3001 cgttggtgca atatcagttg accttacgga acgttcctcc tgagcagcca ggcctgcagg
     3061 ccgaaatcct ggaacgcata ggcttgtccc atcaggacat cggacaatat gccaaagcca
     3121 ttgaggcgtt ctcccgggcc ttggttctga accgtgagtt agggcaggaa caaaatgtca
     3181 cattgctcca gagaaatatc ggggtcaacc tgtttaacct gagccgcgcc agcgaaaccg
     3241 gtggacgcga ggaactcaaa caggctttgg gcagttactt taccagcctg gaccacctca
     3301 ccaccggcgg gggcaaaacg ctttccaaag gaccaggcct gttcaatgtg aatgtggccc
     3361 tcaatgaagg cgggtctcaa gcggccagcg ggttcgacct aaggggcgaa caaaagctca
     3421 tgttcagtta tatcgccagc acctatgagc aattagacga accgggtccc gctcgagatt
     3481 tttatcgcaa aaaactcgcg ctcctcaatc aggtgaagcc ggctacccag gatgcggcag
     3541 ccctgacaga aaaagccatt gtcctcaacc ggctgggagt gctttcccac caattagatg
     3601 aacctgagca ggctatggag tcctttcgtc aatccttgaa ttacacccgg acgttgaata
     3661 tcccgttcgg gacgagcgtc aacatctata acctttcgac attggccgtc gaaagccttt
     3721 tacaaggcca caccccggac cacaaccttg tggaggccat cacctcgggc attcaggatc
     3781 tccaacaaca aaactatgaa gaccgcaatc tcttcttcac cctcaccaac accgctatgc
     3841 tcctctccct tcttcccgac ccatcgccgg acccgcgcct caagccggct gatgccgtcc
     3901 aacgcatgca tgaacagttc acaaccagga cacttccgtg gtcgtattat caaaaggccg
     3961 actcgctcct acagaagccg gccctctttt cagattcaga acgacttccc gctcaatttc
     4021 tggtcaagtt gaaccaggcc gaattggcac gcacatcgga tcagcctcag gtctatcgca
     4081 acattcagga cgacttactc aaactggtgg aagaccggca atcgcccaat agctggttgt
     4141 ggtatctgtc tcaagccgaa gcaaccgctg atcccctgac gcacaagaag cttctcaccc
     4201 aatccgttga ggccgtcctt cgttttcccg cgcaaaccga cccccccacg gggcagacca
     4261 acacctggcc tgcatacgaa cgtctgattc agctctcggt cgaccaattg attaaagaag
     4321 gccggcccga cgacgccttt accgtggcgg agcaactgag cattcgacaa atgaccagcg
     4381 ccgtctatga agcactggga gagacggtct ttctcaaagg cttgggagac tatgaaccgg
     4441 aattacactc gttactcggg gaactccggc aagccagaaa tcacgggaac gtcgcggcca
     4501 ttgaagaact ggcccctgca cttcaggaca cgctctacgc cctgtttgaa gaatatccct
     4561 gggccaccgc gtccttctgg gcctatcctc cgaccaggga cctgatattt ttgggagttg
     4621 acgcgcaaca cccctacctg aaaattctca aagggaggca aggctatcat ggctttgtcc
     4681 ataacggaga cacgctccat tacagccctc tggcggtcac ggaaaaaggg gtcaccgggg
     4741 atgaagaatt tcacaaaagg ctcaaacaat cggcctccgc ctatgtggcg atgccacagg
     4801 aattggaatc caccctccct tcactgacgc tcgatcagaa accgctcacg tgggtcagca
     4861 acttctatga tttcctgaat ggctaccatc agcgcagcct ctttttttct cacatcacca
     4921 ccccaagcac atttctcccc tccacacctt cgagtgcggg ggaaattcca tttgtgctcc
     4981 agcaatttag cggggtcgag acgcacgatc atccgctggc cagccacacc aacattgccg
     5041 cgtttctcaa aaatccggat acgttcgcat ttgaagtaca ggaagctcag aatgttcggg
     5101 aatttgtttc cgtgctcgat tttgccggca gccaacacca ttcgatcctg ctcttcggcg
     5161 gtatcccctc ctcgtcgcct tctccatggg tgctgatctc ttcgctcctg cgggcgggat
     5221 ttcctcatgt catcgtgagc cggacaccgc tcgattccca aacggccgcc aggtttctga
     5281 atcaatatct cacgcatctc aatacgcttc ctcccgatga agccgtcatg gccgcctcga
     5341 aggatctctg gggatcagag acggggaaat accccttccg tcactacgga tttgccggca
     5401 tgggtccgga cgaacgccag gaatatgccg cctccatcta tgaccaggaa gtggcagaag
     5461 ccattcacgc atttgagacc aaagactttc ccgcctcttt gctgcatatc gaacatgcgc
     5521 tcgcccttat cgagcatgcc aaaaaacgac aggacttcaa agaactcacc accctggcgg
     5581 tcgaaaccgc gtttgaaatc agcgattatg aaaagggtct gttctttcaa cagaaactcc
     5641 tggatgcgct cacggctgag actccggcca acgaacgggc tgaggcggaa tatcgccttg
     5701 gcatcctgca ttcccgtctc gaacattttg acctcgccgt acaacacctc gaacaggcca
     5761 accaaatctg ggaacaaggc gaacagcttg atcgcctcgc cgaaggcatg gccaccttgg
     5821 gtattgtgcg tgaaaatatg ggtgcctata cggaagccct ggaccaattc aaccagtcat
     5881 tttccctgta tcaggaaatc ggggaaatgg gccacacggc ctttcaatac cgtcgaatcg
     5941 gtcgtattta ttaccttcga ttgggccggt acgagaaagc ccgtgaaaac tttctggccg
     6001 ctcttaaact gtatcaggaa caaggcgatc cccagggcga agccgaagcg ctgtatgaag
     6061 tgggattgac ctatgaaaaa gtcggtctct tcgaccaggc cgcggaacgc taccaccagg
     6121 ccctctcgat tgcgaaagaa ctggatgacc ccaagctcct cacgacgggc gacctctacc
     6181 tcgccaatgt ggcctggttc caagggaact atcaaacggc ctttcaactc ttaacccagg
     6241 ccgacaaaaa agccgaacag gccaaagacc cgcaattacg cattatggtg aaaaataccc
     6301 gcgggctgat ttactggaca ttgaatgaca ccgacaaagg actggtccat ttacacgacg
     6361 cagtcacgct cagccggtct tccaatattc aaaccgagtt ggcctcttcg ctgaacaatc
     6421 ttggtttgat ctaccggcaa cggggcgacc atgccaccgc gctggacttt tttcaacagg
     6481 cgaaaacgct tgacgagtcc ctgaagagcc aatggggttt aggctatgat catcggaata
     6541 tcggcatctc cttactggcc ctgggacagt tagaagaggc cgaagggcat ttcatcaaag
     6601 ccgagcagat cagcgccgag atcaacaatg tcatcaattg ggtcaaagcc ttgctggaac
     6661 tgggcaatgt taataaggct ctcatccgac ccgaacaggc ccttggctat tacgagcggg
     6721 cacatgagat ctcccagcga tacggcatca aggaggtaga atggcgggcc gcagcgggca
     6781 aagccaccct gttgcgggag gaaggaaagc tcccggacgc cctgacctgg tttaccaaag
     6841 gggtcgaggt ggtggaaggc atgcgggccg cgctgaaaat cgatgaactc cgcaatagtt
     6901 tccaaacgaa caaactggat ctgtaccgtg acatcatcac cctgctcatc accatgaacc
     6961 ggactgatga cgccttcaat tttttagaac gctcccgatc ccggagtttt atcgacctgt
     7021 tgggcaacca aaaactcgca ttcaaaaacc agggcgatcg ggaaacctgg accaaaatca
     7081 acaggctggc ttccacggtg gattcactca aatcggaact gggatcgtat gaggagcccc
     7141 ctgccgatct ccaggatcga tatcgcaata cgaaggccct gtatgaagaa gccatcctgg
     7201 aggtcaaaca gcagaatcca gccctcagca gttttgtttc tgtggatccg ctcaatctgg
     7261 aaggggtcca acagctcctg gcgcctcgag tgggactgct cacctacttt ttgacaaaag
     7321 accagctcta cctgtggctc attaccaaag agcgaactct gtttaaaacg gtttcagcga
     7381 aggaggaaga cctcacccgc ctggtgaccc gctatcggca actcgttcag catctggaac
     7441 cggttgatga tgaattacag aaactctatg ggtctctgat tcaaccggtc gaaccggaca
     7501 tcaaaaacct ggagtatttg ggaattattc ccgatggccc cttacacttt ctctcctttg
     7561 cggcactcaa acacggcccg gcctatctgg tggatgagat cccgcttttt tacgcaccct
     7621 cggctagtgt ctttcaattc acctttgcca aacgtcaagc cgttaaaaat gacaaagttc
     7681 ttgccatcgg aaatcccgat ttgggaaatt tcaattacga tctcccatta gccgaattgg
     7741 aggccaaaag catcaaatgg aattatccca atatggatat tctcaccgga acgaaagcca
     7801 cgaaagaatg ggtggtgaag aatatttccc gatatggcat catccattta gcggcccatg
     7861 gggaattcga tgagggcaat ccgctgttgt cctcactctg gctggcttca gaaaatcccg
     7921 aaaaccgccg actgaccgtc aaggagattt tcggtttgga attaaacgcg gatctggtga
     7981 ccttgagcgc ctgccaaacg ggcctgggca aactggaagc cggagaactc atcggcctga
     8041 accgcgcatt tgtctatgcc ggcacgcatg ccctggtgtc cgccctgtgg cgggtagatg
     8101 atctctccac ctcggtgctc atgaaacatt tttaccggaa ttacgtcacg gcgaataaag
     8161 ccagaagctt gcgtcaagca caactcctcg tcaaaaagga ttttccccac ccgtcctatt
     8221 gggccgggtt tagtttgatc ggagactatc aataatcgta aatcgtgccc gacctcaaag
     8281 ctgaaagggg gcctcatgcc agaccgtatc gctcgggtta tctttggaat tgtctttctc
     8341 accattgttg cctggatgtt tccctcatcc aattgggcgg ccaccctcta cgccaagcag
     8401 gatgacgtca aagtcaatgc ggagaaatct ccgacctccg cggtcatcgc caccctggcg
     8461 ctaggagatg ccgtcaccgt gctagccgaa gaaggtcgac tggccaaagt caaaaccgcc
     8521 acaggaaaaa tcggctgggt gtttaaattc cggctggcag aagaaaaacc ttccacgggt
     8581 ggcggcgggc ttggcctctc aggcctcacg gg
//
LOCUS       DATIAL010000430         6330 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_186234, whole genome shotgun sequence.
ACCESSION   DATIAL010000430 DATIAL010000000
VERSION     DATIAL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186234"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2045
                     /locus_tag="VLA60_13315"
     CDS             237..2045
                     /locus_tag="VLA60_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HSF10388.1"
                     /translation="MAARNALFGSRFPSHSSVQRISRVADIGKGLPVFLFLSLQFFPG
                     IGQDNQLFAQVQPGFDPTGRFGEPPPLEKKTEPLPPKPAPDITLPPVETIPEMKGAPP
                     QIRVMVRKIQVEGSTVFSAEELAKVTAPYENRELSTNDLEELRRALTLLYVNKGYVNS
                     GAVIPDQTLQDGVVTIQIVEGKLTDIQIEGTKYFLPFYFEDRIALSSGPPLNINPLKE
                     KLQLLLQDPRTERLNTELKPGLKPGEAVLHVQVEEASPFKAWVEFNNFQSPTVGESRG
                     LGNIAVQNPFGLGDAFRFTYGQSKGLQPLIEANYIIPLTARDTTLELNFRFNDFNVVT
                     KPFDDLDIRTKSQIYKIALRQPVYRTLNDEIALSLIGEHLENQSFLGGTGFSFQRGAT
                     DNGKAIVSALRFGQEWVHRESNQVLAVRSRFSVGLNVLDATNNKQNSDDPDGEFFVWL
                     GQAQYVRRVDPLGIEFLGGLTLQIANDSLFALEQFAVGGRYSVRGYWENYLVRDNAFL
                     FNIESRIPMFPSFFGPKVAVALAPFIDVGRSWEAKRNTPDPQTLASIGAGIRFSFFNR
                     AHASLYWGQQLNHVENPPDGGVQDQGVHWEFVLDIL"
     gene            complement(2143..2724)
                     /locus_tag="VLA60_13320"
     CDS             complement(2143..2724)
                     /locus_tag="VLA60_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10389.1"
                     /translation="MDEEIHDALTVSIPVPFLGKGRGLGLSVEDMYQDPVFADVYFDD
                     VRSSIQGGVEDFLQETVALLKQEDQWGLRIEGYCDSRGPSAYNLARADYHLRSLAGFL
                     VQLGIPSGRIHPVNFGQTPFSCQSGSERCQEDNLRAERIFSILAVDRFQRGCLARLRL
                     VAGKDSDRATGYLKRPPYLQRIQLASPVITSFR"
     gene            complement(3347..4189)
                     /locus_tag="VLA60_13325"
     CDS             complement(3347..4189)
                     /locus_tag="VLA60_13325"
                     /inference="COORDINATES: protein motif:HMM:NF017829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF928 domain-containing protein"
                     /protein_id="HSF10390.1"
                     /translation="MSQMMTRCRWVAVLGVTLGLSVQAMGGAEFTDQVNRKHKKDVAS
                     VAIEPGFLEETLPSVDRQVVQASDDKQTASSRKESLYQPPPRGAPGGRVGGGTRGPVT
                     DLPVLWALAPDHVGLSSEVQPRLVWYLSKSTTYPLEFTLIDESGVTPIIEKRLSSPTE
                     SGIHIIQLADYDLKLEKGKTYQWFVSLVSDPEHRSADIIGGGMIEVGDVPASLAEELR
                     NANPVEATKLWGQAGFWYDAIGVISTNIQSHPSDSEMHHVRASLLEEVDLDTPAQFDR
                     QHGL"
     gene            4667..>6330
                     /locus_tag="VLA60_13330"
     CDS             4667..>6330
                     /locus_tag="VLA60_13330"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10391.1"
                     /translation="MSRTHGFSNFSRYGLGVWFLLLGVGGIGITSTVAAELSPQDLMA
                     EGSRQYQQGQLEQAARSWTQAARIYEKSGDHPNHIQALIYLSRALLDMGQYQRAQQLL
                     RTAVQSAQETTQQRLMAQALSQLGTLHLNVGELDSALEILQQGLKVSMEIEDRPLMAA
                     ILNDLGNAHAVRRNDTEALAAYTESSILADSLDLQPLSIRAMINAALVEIRQQSAQNG
                     KIHLQQALKKTRTLPDSHEKIQNLLTIGMGLRDLSHQVTEEHATVFTQAAESFREAIG
                     SAKAIGDWKNESYGWGFLGELYEEGGRHEEALQLTQLAIRTSQQGQAPEALYRWEWNT
                     GRHLKNLGRTDEAISAYQRAIDTLQPIRQQLSVGLPQNPASFRESLGALFFETAALLL
                     QQADEVTDATRKEGLLFHTRDTIEAFKAAELQDYFKDDCVEANRGRIQAIDKVSTTTA
                     IIYPILFPDRMALLISLGGTMKKITVPVQEHDLTDKIHLFRTLLEKRTTHQYLPHAQA
                     LYDLLIRPIEPYLSEFGIQTLVFVPDGPLRTIPMGPLHDGEKFLIQ"
BASE COUNT         1695 a         1730 c         1517 g         1388 t
ORIGIN      
        1 caaagaaagt cactaaggag ggagcacggt tgtgtaaacg agacagggaa tgagaagtat
       61 gtcctctttc agcctctcca tattttccca tccgaacttc ttcaaacaat gagggccccc
      121 tcaagaggga cccttaccag acttcatcga ggatcaaata attccctagc aattattgac
      181 ttcttcctgt ataaaaaacc cttacagcag acaacctctt ctactgaatg ccattcatgg
      241 cagcgaggaa tgcgttgttt ggttcaaggt tcccttccca ttcatccgtc caacggatta
      301 gccgggtcgc tgatattggg aaaggactgc ctgttttcct cttcctatcc ctacaatttt
      361 ttccgggaat aggacaagac aatcaactct tcgcacaggt ccagccaggc tttgatccca
      421 ccggacggtt cggcgaaccg ccgcctctag agaaaaaaac cgagcctctc cccccaaagc
      481 ctgcccccga tatcacattg cctccagtgg aaaccattcc tgaaatgaaa ggggcccctc
      541 ctcaaattcg ggttatggtc cggaaaattc aagttgaggg gagcaccgtc ttttcagcgg
      601 aggaactagc caaagtcaca gccccatacg aaaacaggga gttgtccacc aatgatctgg
      661 aggaactgcg ccgggcctta accctgctct atgtgaataa aggatatgta aactccggag
      721 cggtgatccc ggatcagact ctacaagacg gagtcgtcac catccagatt gtcgaaggaa
      781 aactgacgga catccaaatc gaaggcacca aatattttct accgttttat tttgaagacc
      841 ggattgccct gagcagcgga ccaccgctca atatcaaccc gttgaaagaa aaacttcagc
      901 tccttcttca agaccctcgc acggaacggt taaacacgga actgaaaccc ggcctgaaac
      961 ccggcgaagc ggtcctccat gtgcaggtgg aggaggcttc ccccttcaag gcatgggtgg
     1021 aattcaacaa ctttcaatca ccaacggtgg gcgagtcgcg gggtctcgga aatatcgcgg
     1081 tgcagaaccc gttcgggttg ggggatgcct ttcgctttac ctatggccaa tccaagggat
     1141 tgcagcctct catcgaagcc aactacatca tcccgctcac ggcacgagat accaccctgg
     1201 aactgaattt ccggttcaat gactttaatg tcgtcacaaa accgttcgat gatctggaca
     1261 tcaggaccaa gtcccaaata tacaaaatcg ccttgcgtca acccgtctat cgcacactca
     1321 atgacgaaat tgccctttcc ctcattggag aacacctgga aaatcagagt ttccttggtg
     1381 gaacggggtt ctccttccag cgcggcgcaa ccgacaacgg caaggctatc gtgtcagccc
     1441 tccggttcgg gcaggaatgg gttcaccggg aatccaatca ggtcctcgcg gttcgatccc
     1501 ggttcagtgt gggattgaac gtactggatg ccaccaacaa taaacaaaac tcggatgatc
     1561 cggacgggga attttttgtc tggttaggcc aggcccaata tgtccgacgc gtagatcctc
     1621 tcggcatcga atttctcggg ggcctgacct tgcaaattgc caatgacagc ttattcgcac
     1681 tggaacaatt tgccgtcggc ggccggtaca gcgtccgggg gtactgggaa aattatctgg
     1741 tgcgggataa tgcgtttctg tttaacatag agtcccgaat cccgatgttc cccagctttt
     1801 ttggtccgaa ggtggcggtg gcactggctc ccttcataga tgtgggacgg tcatgggagg
     1861 ccaaacgcaa tacgcccgat ccgcagaccc tggccagtat tggcgcagga atccgattct
     1921 ccttcttcaa tcgggcacat gccagtctat attgggggca acagttgaat cacgtggaaa
     1981 atcctcctga tggaggcgtt caggatcagg gagtacattg ggaatttgta ctggatattt
     2041 tgtagccctt ctgaatagaa aggcgttcac taggcagccg gttgttcgga aaagcaacag
     2101 gctttttcgt tcaatggaaa gaaagcgaaa gataacacgc tttcagcgga atgaagtgat
     2161 gacaggggac gcgagttgaa tgcgttgcaa ataaggcggg cgcttcaaat atccagttgc
     2221 tcgatccgaa tcctttccgg cgaccagacg taatcgcgcc agacaccccc gttgaaaacg
     2281 gtccacagcc aggatagaaa atattcgttc cgcgcgaagg ttatcttcct gacaccgttc
     2341 actcccgctc tgacaggaaa aaggagtttg gccaaaattc accggatgaa ttcgcccgga
     2401 gggaatcccc aactgcacca agaaccccgc aagggatctc agatgatagt cggcccgggc
     2461 gaggttatag gcagacgggc ctcgcgaatc gcaatatcct tcaatccgta atccccactg
     2521 atcttcctgt ttcagaagcg cgactgtttc ctggaggaaa tcttcgacac caccctgaat
     2581 ggaagaccgg acatcatcaa aatacacgtc ggcaaataca gggtcctggt acatatcttc
     2641 gaccgatagg cccaaacccc gacccttccc caaaaagggc accggtatag agacagtcaa
     2701 cgcatcatga atttcttcgt ccacgtctac ctgaaaactc aaaaaagcag gagcaatgtc
     2761 cagggtattc gcgagagatt cctgaacaga ccatatcgtc cccaacagaa gggaccatgc
     2821 cagtacaaac ctgtggaatc tattcagatg gaagagaaac atggcagcag gaacgaacga
     2881 ggaccagttt gatagctcat cgagaaaacg cctcagggtt ggtatggttt tggccacacc
     2941 cttttggagt cagtatcggc ccgacaggat gaaactttat tcgagtgaga gaaacgagga
     3001 atcgccattc aggcctagct caacatggaa aatccggagc taaaatgagg tgtgaggtcc
     3061 agcctgagga gtgcgtgaac ggttgttgga taacagtcga gagggagaca acggacggta
     3121 ataaaaaaac ctgcccccgc cacaacggcg gggacaggtt ccataactgc gggtgcaatg
     3181 aaaaggggaa aatgaacctg ctcccttagg acaaaaagcg caggctcaat aacacacggg
     3241 catcacggac tgggggaaat ttctggtcct gtcaacaccc gtccagacaa gaccttcgtg
     3301 gacttgccca taaacacctc caattcacat tttcaaacag ccacagtcat aatccatgct
     3361 ggcgatcaaa ctgcgccggc gtatccaaat caacctcttc aagcaatgac gcccgcacat
     3421 gatgcatctc ggaatcggaa ggatgagact gaatattggt ggaaatcacg cctatggcgt
     3481 cataccaaaa tccggcttgc ccccatagct tcgtggcttc aaccggattg gcgttcctca
     3541 attcctcggc cagcgaggcc gggacatcac ccacctcaat cattcctccg ccgataatat
     3601 ctgccgatcg atgctcggga tcagagacca gggacacaaa ccattgatag gttttccctt
     3661 tctccagttt cagatcgtaa tcagccaact gaatgatgtg aatcccggat tcagtgggag
     3721 aggaaagccg cttctcaata atcggtgtca cccctgattc atcaataaga gtaaattcca
     3781 aagggtaggt tgtcgacttt gataaatacc acaccaatcg tggctgcacc tcactcgaca
     3841 atcctacgtg atctggagcc agggcccaca gtaccggtag atccgtcaca ggacctctgg
     3901 tgcctccacc aactcgtcct cccggagccc ctcgtggtgg aggttggtac aacgattctt
     3961 tccgggatga agccgtctgc ttgtcatccg atgcttgaac aacctgccga tcaacagaag
     4021 ggagtgtctc ctccagaaac cctggttcaa tggctacaga cgcgacatcc ttcttatgtt
     4081 tgcggtttac ctggtctgtg aattccgctc ctcccatggc ctgaacacta agaccaaggg
     4141 tcacgcctaa gacagccacc cacctgcatc gtgtcatcat ctggctcata atggtcctcc
     4201 tctcttcgtc cggaccgtcc tcccggtaca ttttcgcgta aaagtaagac gttctcacat
     4261 cggctcacac tcatgaatac agctacataa cgcaagcttc gttccagaag ccaaattttc
     4321 cccacttgga gacaatccat tcgaaccccg tgccattgct aaagatttca tgaataccct
     4381 ctctcacatg gtcttgccgt ctaaggtatt tggatatctc aactgtctac ctggtgtatt
     4441 gtgtgtctac tttgagaaca atttcaagag aaaggggttt accctcacaa gctgacgacc
     4501 gtagaccttc atcgttaaaa tcgctttcct cttccagatt cacaaccaga cccacgagac
     4561 ccctcaaaaa cgccccgtat cagaaaccct tactttgacc ttattgacga caattcctag
     4621 aatgggactg ttgaatgcat agataacttc agagaaaggg tcggccatgt cccgaactca
     4681 cggattttcc aacttttctc gttatggtct tggggtgtgg ttcttactcc tgggagtcgg
     4741 cggaataggg ataacgagta ccgttgcggc tgaactgtct cctcaagatt tgatggccga
     4801 gggaagccgg cagtatcagc agggccagtt ggaacaggcg gcccggtcct ggactcaagc
     4861 cgcccggatc tatgaaaaat ccggagatca tcccaatcac attcaagcgc tcatttatct
     4921 ttcaagagcc ctgctcgata tgggccaata ccaacgggcg caacaactcc ttcgcacagc
     4981 cgtccaatca gcacaggaga ccacccagca acgcctgatg gcccaggcac tcagccaatt
     5041 gggaaccttg catctcaatg tgggagaatt ggattcggca ctcgagatcc tgcaacaggg
     5101 gctgaaggtc tccatggaaa tcgaagatcg acccctcatg gctgccatcc tcaatgatct
     5161 gggcaatgca catgcggttc gccggaacga cacagaagcc cttgcggcgt atacagaatc
     5221 cagcattctt gctgattcac tggatttgca acctctctcc attcgagcca tgattaatgc
     5281 agccctggtg gaaataaggc agcagtccgc acaaaatggg aaaatccatc tgcaacaggc
     5341 attaaagaaa acacgaacac ttccggactc acacgaaaaa attcaaaacc tgctcaccat
     5401 cggaatggga ttgagagacc tcagccacca ggtcacagaa gaacatgcca cggtattcac
     5461 gcaggccgct gagtcatttc gggaagccat aggcagcgcg aaagcaattg gagactggaa
     5521 aaatgaatca tatgggtggg gatttttagg agaattgtac gaagaagggg gccgccatga
     5581 ggaagccctc caactcacgc aattggcgat tcggacgagt caacagggcc aggccccgga
     5641 agccctctat cgctgggaat ggaacacagg acggcacctc aaaaatctgg gtcggacgga
     5701 cgaggccatt tcagcctacc agcgagcgat cgatacgctc caacccattc gtcagcaact
     5761 gtctgtcggg cttccccaga atccggcatc atttcgagaa tcgctgggag ccttattttt
     5821 tgaaaccgct gccctgctac tccagcaagc ggacgaagtg acagatgcca caagaaaaga
     5881 agggcttctg ttccacaccc gggataccat tgaagcattc aaggccgcag agctccagga
     5941 ttacttcaaa gacgattgcg tggaagcgaa caggggcaga attcaagcca tcgataaagt
     6001 atccaccacc acggcgatta tttatccgat tctcttccct gaccggatgg ccctgctaat
     6061 aagccttgga ggaacgatga aaaaaatcac cgttccggtt caagaacatg acctgacaga
     6121 taaaattcac ctgttccgga ccttattgga aaaacggacc acccatcaat atctacctca
     6181 tgcgcaggcc ctgtatgacc tgcttatccg gccgattgaa ccgtatttga gtgaattcgg
     6241 gatccaaacg cttgtcttcg tcccggatgg accattgcga accattccca tggggcccct
     6301 ccatgacgga gaaaaattcc tcattcaaaa
//
LOCUS       DATIAL010000431         6147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_616288, whole genome shotgun sequence.
ACCESSION   DATIAL010000431 DATIAL010000000
VERSION     DATIAL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616288"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..663)
                     /locus_tag="VLA60_13335"
     CDS             complement(124..663)
                     /locus_tag="VLA60_13335"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HSF10392.1"
                     /translation="MRRWTVFSLMFVMLGLGFALVAPLGLAADAAKEMAVKYILATSK
                     AARTVYVKGIMADATKGGMKPSEDWVKDDHGIMLPAQFVKELGKEIKEFDLSLVGTDP
                     LYASNAAKSAKEKDMLVELAKGKEKVLVAEDGGATVGMSADYAIADSCADCHNKHPKT
                     TKKDWKKGDFMGAIVVRLR"
     gene            complement(900..2708)
                     /locus_tag="VLA60_13340"
     CDS             complement(900..2708)
                     /locus_tag="VLA60_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF10393.1"
                     /translation="MGKVWNSIGIGPKVVMTIMVVFSGLLAAGAYLLGQQLHADLERV
                     LSDQAYIVQKQIEVTRAYVTKEFVVKAKAAGMNTGVDHSAPDTIPFPATFARETSELL
                     AEEGVFNARIVSLTPLNPKNAPQDSFEKEALVALASGQKEFTKNDTVNGKMMFRRATP
                     DLATAQACIGCHEGKKIGDMLGAVSVQIPMDGPAAQLQGNLSNMYMGILAVGVLMMGL
                     LYLMIAKLVVQPMKSLESMAVRAREGDLTGRASVNSQDEIGRTSESFNMMFDRVSEVI
                     ASVKTAVSGITTGTSEINAGTSDLAGRTSAQAGALEETSASMEEMTSTIKQNADNAKQ
                     ANQLAVAAREVAEKGGAVTDKAVEAMDEINKSSKKIADIINVIDEIAFQTNLLALNAA
                     VEAARAGEQGRGFAVVASEVRNLAQRSATAAKEIKTLINESVQKVGGGSELVNRSGQT
                     LAEIVNSVKRVTDIISEISAASQEQAAGIDQVNKAVMQMDQGTQQNAALVEQATSASQ
                     SMAQQAEDLLRQVEFFKVQTESGSQRSETTAKTRAAVHAQSTGGTGSQYSRAVSSITG
                     TAQGKHSPNKREVPVGVGSSASNGHKKSGDDFFEEF"
     gene            complement(2750..4978)
                     /locus_tag="VLA60_13345"
     CDS             complement(2750..4978)
                     /locus_tag="VLA60_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HSF10394.1"
                     /translation="MVNTIFSLGITTKLVVLFAIFGIVPMAAVGYLGSSATGEMETAA
                     GLRVQAMAETIGDKIDRNLFERYGDPQSFGLNRIVGERYHWYSKENNAIASAMNQYVA
                     TSGIYYLTILVDPVGDVMAVNSKDAKGNPIDTTAIYSKKYSDAPWFKAMEAKEFTTKQ
                     PFTAPGNETATGTFIEDVHVDEDVKAVYSGDDGLTLGFSAPVYVDGDLIGYWSNRVKF
                     SLVEEIVQTAYQELKGAGWAGSEISLLGDKGQVLIDYDPFNQGTEDMTHDFEKTLFKL
                     NLAETGVESAKLAVAGKTGYQLALHDGKNIWQMGGYTHLKGALGYPGMNWSVLVRVPK
                     AEATAAAIAFNRNLTMTAIICLALILIVGLWVGRRVAKSLVHISEVAQRAADGDLIRR
                     VTVTSNDELGSMGKAMNAMLDNLVKVVSEVRQAAEHVSTASGEITQGNEDLSQRTSAQ
                     AGALQETSASMEQMTSTIKQNADNAKQANQLAVAAREVAEKGGAVTDKAVVAMDEINK
                     SSKKIADIINVIDEIAFQTNLLALNAAVEAARAGEQGRGFAVVASEVRNLAQRSASAA
                     KEIKALINESVQKVGDGSELVNRSGQTLGEIVNSVKRVTDIISEISAASQEQAAGIDQ
                     VNKAVMQMDQGTQQNAALVEEATSASQSMKQQATELLNQVAFFKLEETGHRQFAGGQR
                     GSGATSVLANGKKSSAVSQPLHSVKAVSPQKSTAKSQPVGVGSSNGHDRRQRENDFFE
                     EF"
     gene            complement(5065..5604)
                     /locus_tag="VLA60_13350"
     CDS             complement(5065..5604)
                     /locus_tag="VLA60_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSF10395.1"
                     /translation="MTTATSEPTEQRSIRTTYASDDQQYLTFNLADEYYGVDILKVQE
                     IKGYTTVTRIPNTPDFLKGVLNLRGTIVPIVDLRMKFGMGVTEPTSFTVVVVVNVRNR
                     VMGFLVDAVSDVLDMNAKDIQAPPDMGTSVDVTFIAGIGNAQDRLVTLLDIDRVLTEE
                     EVIVVENIQDVEEKAEVHA"
     gene            complement(5746..>6147)
                     /locus_tag="VLA60_13355"
     CDS             complement(5746..>6147)
                     /locus_tag="VLA60_13355"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HSF10396.1"
                     /translation="GQDNYIIPLIAVTESIRPKPNELQRIVGRGEVVNLRGEWVPVVK
                     LYEAFTLTPDFTDPSQALLVIVETDGRRLAVLVDELTGQQQVVIKSLEQNYRKVEAIS
                     GATILGDGQVALILDVPGLAKLARVGHIAAA"
BASE COUNT         1398 a         1811 c         1348 g         1590 t
ORIGIN      
        1 acccatagcg gggaattcta gccggagatt ttttaggata aagaaacgat ggggggaaaa
       61 gaggagagga aggggtctaa gccttcctcg cctctcttta agacaaatga tcttaaaact
      121 aaattatctc aatcgcacca caatggctcc cataaagtca cccttcttcc aatctttctt
      181 ggtcgtctta ggatgtttgt tatggcagtc ggcacaactg tccgcgatag cataatcggc
      241 cgacatccca accgtcgctc cgccatcttc cgccactaac actttttctt ttcctttggc
      301 taattccacg agcatgtcct tttccttggc acttttcgcg gcattggagg catacaaggg
      361 atcggtccca accaaagaca aatcaaactc ctttatttct ttcccgagtt ctttcacgaa
      421 ctgagccggc agcattatgc cgtggtcatc cttcacccaa tcttcagacg gtttcattcc
      481 acctttggtc gcgtcggcca taataccctt gacatatacc gttctggcag ccttagaggt
      541 ggccaagatg tacttgaccg ccatttcctt agccgcgtct gcggctagac cgagcggcgc
      601 aaccaaggca aagccaagac ctaacatgac aaacattagt gaaaatacag tccatctcct
      661 cataaccatc ctcctcttgt aagtataggg aaacatcatg cagatgagtc cgacatggtt
      721 atgggacatt cagcatttcc tccataacct ggttcaaaca caatgttaat aaacactcat
      781 agaatcctct ccgggttttc gggaaaaaat ggatcagact ttaggagaga atttcacagg
      841 cgttgaagtg aaagtcatga tttccccttt tcgtccagct ccaagttatg aacagtcttc
      901 taaaattctt caaaaaaatc atccccggac tttttatgcc cgttggacgc cgaactccca
      961 acacccacag gaacttctct tttattggga gaatgctttc cctgagctgt gccggtaata
     1021 gacgagaccg cacgagaata ttgagagccc gttccgccgg tcgattgagc gtgaacagct
     1081 gcgcgggttt ttgctgtggt ctccgaacgt tgagacccgg actcagtctg gaccttgaaa
     1141 aactcgacct gccgcaacag atcctcagct tgttgcgcca tggactggga cgctgaagtc
     1201 gcctgttcca ccaaggccgc attttgttgt gtgccttggt ccatctgcat caccgcctta
     1261 ttcacctggt cgatccccgc cgcctgctcc tgggacgccg cactgatttc cgaaataata
     1321 tccgttaccc gtttgaccga gttcacaatc tcggccagcg tctggcccga gcgattgacc
     1381 aactcactgc cacccccgac tttttgcacc gattcgttga tcagcgtctt aatctctttg
     1441 gcggccgtgg ccgaacgttg cgccagattt ctcacttccg aggccactac ggcaaaacct
     1501 cgtccctgct cgcccgcccg tgccgcttcc acggcggcat tcaaagctaa gagattcgtt
     1561 tggaaggcga tttcgtcgat cacattaata atatccgcga tctttttgct gctcttatta
     1621 atctcatcca tggcctctac ggccttgtcc gtgaccgcgc cacccttctc cgccacttcc
     1681 cgggcggcca ccgccaattg gttcgcctgc ttagcgttat ccgcattctg tttaatggtt
     1741 gaggtcatct cttccatgga cgcactggtt tcttctaagg ctcctgcttg tgccgaagta
     1801 cgcccggcga gatccgaggt accagcatta atctcggaag ttccggtcgt aataccggac
     1861 actgctgttt ttacagacgc aatcacttca ctcacccggt caaacatcat attaaacgac
     1921 tcactggttc ggccgatttc gtcctgacta ttcacgctgg cacgaccggt cagatctcct
     1981 tccctagctc tcacggccat ggactccaga ctcttcatcg gctggaccac caacttcgca
     2041 atcatcagat acaacagccc catcatcaag acacccacag ccagaatccc catgtacata
     2101 ttgctgagat tcccttgtaa ttgtgccgcg ggaccatcca tgggaatctg tactgatacc
     2161 gctcctaaca tatccccgat ttttttgccc tcgtgacagc cgatacaggc ttgagcggtc
     2221 gccaaatccg gggtcgcacg gcggaacatc attttcccat tcacggtatc gtttttggta
     2281 aattcttttt ggccgctcgc gagggcaaca agggcttctt tctcaaagga atcttgtgga
     2341 gcatttttgg gattcagggg cgtgagactc acgatcctgg cattaaagac gccttcctca
     2401 gctaagagct cagaggtttc acgagcaaat gtggccggaa agggaatcgt atcgggtgca
     2461 ctatgatcca cgccggtgtt catcccagcc gccttagctt tcaccacaaa ctctttggtc
     2521 acataggcac gggtgacctc aatttgtttt tgaacaatat acgcttgatc ggataagacc
     2581 cgttcaagat ccgcatgcaa ttgctgaccg agcaaatatg cccccgcagc cagcaacccc
     2641 gagaagacca ccataatagt catcaccact tttggaccaa tgcctattga attccagact
     2701 ttacccatgc gacctcctaa tttccttgtt aaataaacag actggtttgc taaaattctt
     2761 caaaaaaatc gttctcacgt tgacggcgat catggccatt cgagctgccc acgccaaccg
     2821 gttgggactt agccgttgat ttttgaggag agacagcctt cactgagtgt aatggctgac
     2881 tcacagcgga agactttttg ccgtttgcta acacagacgt ggctccagat ccacgttgtc
     2941 cccctgcaaa ctgccgatgt cctgtctctt ccaacttaaa aaacgcgact tgattcaaca
     3001 gttccgttgc ctgctgcttc atggactggg aagccgacgt ggcttcttcc accagggccg
     3061 cattctgctg cgtgccctga tccatctgca tcaccgcctt attcacctga tcgatccctg
     3121 ccgcctgctc ctgggacgcc gcactgattt cggaaataat atccgtcacc cgtttgacgg
     3181 agttcacgat ctcgcccagc gtctggcccg agcgattcac taactcactg ccgtcaccca
     3241 ccttttgcac cgattcattg atcagggcct tgatctcctt ggcggccgac gccgaacgtt
     3301 gcgccagatt ccgtacttcc gaggccacca ccgcaaaacc ccggccctgc tcgcccgccc
     3361 gtgccgcttc cacagccgcg ttgagtgcta aaagattggt ctggaaggcg atttcgtcga
     3421 tcacattgat aatatccgca attttcttgc tgctcttatt aatctcatcc atagccacca
     3481 cggccttgtc cgtgaccgcc ccacctttct ccgccacttc ccgggcggcg acagctaatt
     3541 gattggcctg cttggcgtta tccgcatttt gcttgatggt ggacgtcatc tgctccatcg
     3601 acgcgctggt ttcctgcaaa gcgcccgcct gggcggaggt gcgttgcgaa agatcttcat
     3661 tgccttgggt gatttcacct gacgcagtgg agacatgttc agccgcttgg cggacttcag
     3721 acaccacctt caccaaatta tccaacatgg cattcatcgc cttacccatg cttcctaatt
     3781 catcattgga ggtcacggtg acgcgccgaa tcaaatctcc gtcagccgcc cgttgagcca
     3841 cctcactgat gtgaaccaga ctctttgcca cacgacgccc aacccacaat ccgacaatca
     3901 aaatcagagc cagacaaata atggcggtca tggttaaatt tcgattgaat gcaatggccg
     3961 cagccgtagc ttccgcttta ggaactctga ccaggaccga ccaattcatc cctggatagc
     4021 ccaaggcgcc tttcagatgg gtatatccac ccatttgcca gatgttcttc ccgtcatgga
     4081 gggccaattg atagcctgtt ttcccggcaa cggccaattt tgcgctttct accccggttt
     4141 cggcgagatt gagtttaaac aatgtttttt caaaatcatg ggtcatgtcc tctgtgcctt
     4201 gattgaaagg atcataatct atgaggacct gtcccttatc gccgagcaga gaaatttccg
     4261 atcctgccca tccggcccct ttaagctctt gatacgcggt ttgcacgatt tcttctacca
     4321 gagaaaattt gacccgattg ctccaatacc cgatcagatc gccatccaca tacaccggag
     4381 cagaaaaacc cagggttaac ccgtcatctc ccgaatacac cgctttcaca tcttcatcga
     4441 catggacatc ttcaatgaag gtcccggtcg ccgtctcatt gccgggagcg gtaaagggtt
     4501 gtttggtggt aaattctttg gcttccattg ccttaaacca cggggcgtcc gaatattttt
     4561 tggaataaat tgccgtagtg tcgatcggat tccctttcgc atccttggag ttcacggcca
     4621 tgacatctcc aaccgggtct accaatatcg tcaaataata aataccggat gtcgcaacgt
     4681 attggttcat cgcactcgca atggcattat tctccttgct ataccaatgg tagcgttcac
     4741 ccacgatcct gttcagtccg aaggattgag gatccccata ccgttcgaac aaattccggt
     4801 cgattttatc cccgatagtc tccgccatgg cttgcacacg cagacccgca gctgtttcca
     4861 tttccccggt agccgaagat cctaagtacc ccaccgcggc catcggtaca atgccaaaga
     4921 tggcgaacaa caccaccaac ttggttgtaa ttcccaaact gaaaattgtg ttcaccatcc
     4981 tgttcataaa atcctcgctg acttctttaa agtggttgtt cgccgccggt gtgtcggacc
     5041 atccatggtc cctttcgttt ccggtcaggc atggacctca gctttttctt ccacgtcttg
     5101 aatgttttcc acgacgatta cttcctcctc cgtgagaacc cgatcaatgt ccaacagggt
     5161 cactaagcga tcctgggcat taccgatgcc ggcgataaac gtgacatcga ccgaggtgcc
     5221 catgtccgga ggggcctgaa tatccttggc attcatatcc aacacatcgg acaccgcatc
     5281 gactagaaag cccatcacac gattccggac attgaccaca accaccacgg tgaacgacgt
     5341 gggttccgtc acccccatgc cgaatttcat gcgaagatcc acaatgggca caattgtgcc
     5401 acgaaggttg agcacccctt taagaaaatc cggggtattg gggatcctgg tcaccgtggt
     5461 gtatccctta atctcctgca ctttcagaat atccactccg taatattcat ccgccagatt
     5521 gaaggtcaga tattgttgat cgtcgctggc atacgtcgtt cgaatcgagc gttgttctgt
     5581 gggttcagac gttgcggtgg tcatgattag gctcctttgt tatgaacatc atctttatgg
     5641 ctcaatactt gtcatccttt tccctcgtaa agcgtgaagc gtaaggcgtg agaaggaaag
     5701 ctgtcttgtt cttcacgcct cacgtctgct tcgcctcacc gtttttcacg ccgccgcaat
     5761 gtggccaacc cgtgccaatt tggccaatcc cggtacatcc aagatcaagg cgacctgccc
     5821 gtcccccaga atggtggctc cggaaatagc ctcgaccttg cgataatttt gctccaaact
     5881 ctttatgacc acttgttgtt gccccgtcag ttcatccacg agcacggcca atcgccgacc
     5941 atcggtttcc acgatcacca acaaagcctg ggaaggatcg gtaaagtcgg gcgtgagggt
     6001 aaacgcctca taaagcttga ccaccggcac ccattctcca cgaagattga ccacctcgcc
     6061 tcttcctacg atccgttgca actcattggg tttagggcga atggattcgg tgaccgcaat
     6121 aagcgggata atgtagttat cctgacc
//
LOCUS       DATIAL010000432         7715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_403017, whole genome shotgun sequence.
ACCESSION   DATIAL010000432 DATIAL010000000
VERSION     DATIAL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403017"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..959
                     /locus_tag="VLA60_13360"
     CDS             399..959
                     /locus_tag="VLA60_13360"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSF10397.1"
                     /translation="MGGIGIFCLLLGCDSGYSESSFADAPSSHSVASRPSKGSPAPDF
                     RLMDMKGKAVSLSDFKGKVVLLNFWATWCGPCRIEMPAMEALYRSMRSKGLEIVAVSV
                     DQQGTAVTRPFQEAMGLSFPILHDQDYEVGLTYGARTLPMTFAIDRQGIIRQVVFGSR
                     DWNSPEARRGIAEVLQEPIPESSQRM"
     gene            967..1716
                     /locus_tag="VLA60_13365"
     CDS             967..1716
                     /locus_tag="VLA60_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HSF10398.1"
                     /translation="MIDSISQVSLLAAFSAGLLSFVSPCVLPLVPSYLSYITGLSVEN
                     LAKVEERERFKSAIILNALLFIAGFSTVFIAFGASASLMGQLLYDYQDVIRKVGGVLI
                     IIFGLYLLGILKLSFFMTERRLMHFETRPVGYLGSFLIGTAFAAGWTPCVGPVLGAIL
                     AYASTTESMSSGVMLLSAYSLGLGLPFFLTAFGMDTFLSYFKNLRSYLGGVSFISGGL
                     LVAVGVMIYADSLTLITSFLERNGIGWYIGQ"
     gene            2178..3197
                     /gene="galE"
                     /locus_tag="VLA60_13370"
     CDS             2178..3197
                     /gene="galE"
                     /locus_tag="VLA60_13370"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HSF10399.1"
                     /translation="MILVTGGAGYIGSHTCVELLHAGCDVTVFDNFSNSHPESLARVQ
                     RITGKSLRLIRGDCRDRDAVVAALRESGATAVIHFAGLKAVGESVQQPLAYYDNNVVG
                     SLRLLEAMGECGVKQLVFSSSATVYGDPQRLPLTEEHPLSATNPYGRTKRMVEEILRD
                     LHRSDPSWRIGILRYFNPVGAHDSGLIGEDPQGIPNNLLPFVAQVAVGRLERLNVWGA
                     DYPTPDGTGVRDYIHVVDLALGHLKALEALARLDVQKECLTVNLGTGNGYSVLEIVRE
                     FETASGKPIPYKIAPRRPGDIASCYADPNQAFTLLGWRAERGLSEMCADAWRWQRDNP
                     QGYAV"
     gene            complement(3658..4716)
                     /gene="bamD"
                     /locus_tag="VLA60_13375"
     CDS             complement(3658..4716)
                     /gene="bamD"
                     /locus_tag="VLA60_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HSF10400.1"
                     /translation="MKFPVNFRSLLLPFLTALIMIAGCSSTPKPAEHADTTSKQQVSS
                     TDEQIFVGDSVEMSYDPNVIMKRAESFFDKESYAESIVEYKHFLDLHRNHILAPYAQY
                     KIGVSHFKQYRTVDRDPEPLEQSIQSFEKLLQEFPASRYETEAKQTILICKEQLAQRH
                     LMVGHFYLKRGSYLAAAHRFEKIIKEFPELETAGDAMFHLAKTYQNLGIEEWSQEWLL
                     TLVNEHPNNPYHSDGKELLAKLEKKNPTLLLASLPSDPSPNEHNTPNPSAIGQRPNNQ
                     RLVRTISHTPESPLLPAATAPTVTPAMLSARTASEPPLSKTACTIGTWCDSPSSSATT
                     PPMQVSSSAKSCKTGEWC"
     gene            4962..5954
                     /locus_tag="VLA60_13380"
     CDS             4962..5954
                     /locus_tag="VLA60_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HSF10401.1"
                     /translation="MNANLSRIVGTGSFLPGRQVDNQEVADLLGIEPSYIFRVSGIRA
                     RFWADSHEECSFLAEQATRRALDQAGLVPEDLDAILVSSTSPEMIFPSTACLLQARLG
                     IRGIPAFDLSASCSGFLYGLSMADCFIRAGQFRRCLVVASEIKSRSLDVKDLSTTILF
                     GDGAGAAIVERSSNSDMGVVSIRIHADGTYHDLVKIAGGGSRQPLSQDVLDASAHTLR
                     IRGSRVFRIAIRRLGQAVRDHLAQEKWDVSQLDQVIFHQANGRMLTQFCENIGVPPER
                     CLSMIEQVGNTSSASLPMALDHANRNQKLKTGDRVMLGAFGGGLTWGTALVRWG"
     gene            6103..7041
                     /gene="ispH"
                     /locus_tag="VLA60_13385"
                     /gene_synonym="lytB"
     CDS             6103..7041
                     /gene="ispH"
                     /locus_tag="VLA60_13385"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HSF10402.1"
                     /translation="MKIFLANPRGFCAGVDRAIEIVDLSLKTYGAPIYVRHEIVHSRH
                     VVESLRGKGAIFVEELNEVPDGAIVIFSAHGVAKEVWEESRRRNLKVIDATCPLVIKV
                     HNEVNRDYSNEYELILIGHAGHPEVVGTLGQVPDKFHLVSSVEDVDSLEVENPLHLSY
                     VTQTTLSVDECRDIVAALHGRFPGIKGPHQEDICYATQNRQNAVKELSRFVDVILVIG
                     SPNSSNSNRLRELGERCGIPSYLIDSYQDIQTQWLEGVNAIGIAAGASAPEVLVAQVI
                     KFLKEHGATSVEELTVVEENVEFLLPKELLMAKVPR"
     gene            7363..>7715
                     /locus_tag="VLA60_13390"
     CDS             7363..>7715
                     /locus_tag="VLA60_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF10403.1"
                     /translation="MYLRTLVVSGFKSFAEAKIDFPNGITAVVGPNGTGKSNIVDAIL
                     WAMGEQSVKSLRSERMEDVIFNGTEQRKPMGMVEASLIFSEVTPRELEPVSAILEGLD
                     QPTDVMITRRLYREG"
BASE COUNT         1701 a         1685 c         2172 g         2157 t
ORIGIN      
        1 aacagtcgct agcgataggc ttgtatcgtt cacaatttcc cgggtctcgc aggataggga
       61 gtttcgtgta cggctacgta aggtcttgcc ggaaaaaatg aaaatcatat ttggctttcc
      121 cgttttcgtc gagaccttgt aatatgtata gatctcctca aaaatatgaa attgacacgc
      181 acctgatcca ataaaatcca aacgatcctg gtgggtcgac ccgtatgaaa tgaattgcgt
      241 tgacgatgaa atttatcggg tcttatgatg aaggttgaag gagtttgccg gtatagtgat
      301 taggcgatgg ttgaatagtc ttgtggggag gcagtgaatt gaaaaccata cttgggttga
      361 gggccgcatg ccattacctc tcgaagtctg gactcttttt gggagggata gggatttttt
      421 gtcttcttct aggctgcgat tccggctatt ctgaaagttc ttttgccgat gcgccatctt
      481 ctcattctgt agcctcccgt ccttcaaagg gatcccccgc tccggatttt cgcttgatgg
      541 acatgaaagg aaaggctgtt tcgctttccg attttaaggg aaaagtggtg ttgctgaatt
      601 tttgggcgac ctggtgtggg ccgtgccgga ttgaaatgcc cgcaatggag gccttgtatc
      661 gcagtatgcg atccaaaggg ttggaaatag tggcggtctc cgttgaccaa cagggcactg
      721 ccgtgacaag gccttttcag gaggcgatgg gcttgagctt tcctatcttg catgatcagg
      781 attatgaggt agggttgacc tatggcgcac gtacgctccc tatgactttt gcgattgatc
      841 gtcaaggcat catccggcag gtggtcttcg gatcccgtga ttggaacagc cctgaagctc
      901 gcaggggaat tgccgaggta cttcaagagc cgattccaga gtcttcccaa cggatgtaag
      961 aacaatatga tcgattcgat cagccaggtg tctttgcttg cggcatttag tgccggattg
     1021 ctgtcttttg tttctccatg cgtcctcccc ctcgtccctt cctatctatc ctatatcact
     1081 gggctctcgg tggaaaatct cgccaaagtt gaagagcgag agcgatttaa gtccgcgatt
     1141 atcctcaatg ctctgttgtt tattgccggg ttttccacgg tctttatcgc gtttggggca
     1201 tcggccagcc tgatgggaca attgttgtat gactatcaag acgtgatccg gaaagtcggg
     1261 ggtgtcttga tcattatttt cgggctatat ttgcttggga ttttgaaatt aagttttttt
     1321 atgaccgaac gacgcttgat gcattttgag acgcgtcccg ttggctacct gggttcgttc
     1381 ctgatcggaa ccgcatttgc cgccggctgg acgccttgcg taggaccggt gttgggggcc
     1441 attctggcct atgccagtac gacggagtcg atgtccagcg gggtgatgct cctatcggcc
     1501 tattcgttag ggttgggtct cccgtttttt ctcacggcat ttgggatgga tacgtttttg
     1561 agctacttca aaaaccttcg gtcctattta ggcggggtgt cgtttattag cggcggactg
     1621 ttagtggcgg tcggcgtcat gatttatgcg gattctctga cactcatcac cagttttctt
     1681 gaacgtaacg ggattggttg gtacatcggc caataaaaag gcctacccga cctggcctat
     1741 tctcctcttt cttctttcct cgtctttcca ctcgcttgta tagtttccgg aacgatggtg
     1801 ggcttaagca gatttaggag tatattggag caaccaatat tcatggggga gcgatggtct
     1861 tctcttggtt atggagtggt gggggccatg ggaaaaaact ggacagaagt ttcatgaacg
     1921 gctcttgttt ttttgagcca ccgattctca gtcacatgta ttgatggtcc tcaattcacc
     1981 tctattcctt accgatctat ttcttgaggc acctttcttc tcagtggagg gtcccaccac
     2041 attttttgag actctataat acggaatcgt tggcttgcca tcattgaagt gtctcagcct
     2101 ccttcacgtg atgtaccaag gatcaacatg aaaagaaaca gcagcaggct atctcaattt
     2161 caatgggagc gttctcaatg attttggtaa ccggcggcgc cggctatatc gggtcacata
     2221 cctgcgttga gctcctccat gcgggctgtg atgtgacggt ctttgacaac ttctctaata
     2281 gccatcctga gtcgttggcg cgggtgcagc gtatcacagg gaagtctctt cgtctgatac
     2341 gcggggactg ccgtgatcgg gacgcagtgg tggcggctct gcgcgaaagc ggggcgaccg
     2401 cagtcatcca ctttgccggc ctcaaggcgg tgggagaatc ggttcagcaa ccgttggcct
     2461 attatgacaa caatgtggtc gggtctctgc gcctgttgga ggccatgggc gagtgcggcg
     2521 tgaaacaact ggtgtttagc tcttctgcca cggtttatgg ggatccgcaa cgccttccgc
     2581 tcaccgagga gcacccgcta tctgcgacca atccctatgg tcgaacgaaa cggatggtgg
     2641 aggagatctt gcgtgaccta caccgcagcg atccctcctg gcggatcggc atcttgcggt
     2701 actttaatcc tgttggggcg catgatagtg gtctgattgg tgaagatcct cagggtatac
     2761 cgaacaattt gttacccttc gtggcgcagg ttgcagtggg acggctggag cgtctgaatg
     2821 tatggggtgc cgattatcct actcccgatg ggaccggagt gcgggattat atccatgtag
     2881 tggatttggc actggggcat ctcaaagcac tagaggcatt ggctcgactg gatgttcaga
     2941 aagaatgctt aacggtcaac cttggtacgg gcaacgggta cagtgtcctg gagatcgtgc
     3001 gggaatttga aacggcgagc ggcaagccga ttccctacaa gatcgcacca cgccgccccg
     3061 gtgacattgc ctcttgctat gctgatccga atcaagcgtt cacacttttg ggttggcgag
     3121 ctgaacgtgg actcagtgag atgtgcgcgg atgcctggcg atggcaacgt gacaaccctc
     3181 agggctatgc tgtctgattt atccccatag cgatctgcag gcctctggta gtcgggccgc
     3241 tgagagtcta gtgcccaggg gttctggtaa atggcgtatt accaggcgaa atcttcgtgg
     3301 taccgtcgcg tgatcggaat tctcgcgtgg tcatagagga tctccataga ggccgcagta
     3361 ccacactggc ttcccccaca acatcgcatt gcatgatcaa gctatagaag attcgggtcc
     3421 gtgaagcccg gagaagttcg cgtgggagcc gtctattggg gggcacattc cgcaagcggt
     3481 caggaggttg cccgatgaat attttttgaa tgtgatgtcc ttcgttgaca tcgcagagga
     3541 attgagcttc atgggacagg tacttggggc ggataacggg aaattgcgcc catgaggcca
     3601 attggcctct atacgattac tcgagaacac ggttcagcta agtaaggacg tagcgggtta
     3661 acaccattcg ccggttttac aggatttcgc gctactcgat acctgcatgg gtggtgttgt
     3721 ggccgaggaa gatggtgagt cacaccacgt gccaattgta caagctgtct tggagagagg
     3781 cggctccgaa gccgtgcggg cgcttagcat ggccggcgtc actgtcgggg cggtggctgc
     3841 gggtaacaat ggggattccg gggtatggga tattgtcctt acaaggcgtt gattgttagg
     3901 gcgttgtcct attgccgagg gattgggggt gttgtgctca ttcggcgagg gatcggaagg
     3961 aagggatgcg agaagaagag taggattctt tttttccagc tttgccaaga gttctttgcc
     4021 gtctgagtga taggggttgt tgggatgttc gttgaccagg gtaagtaacc attcctggga
     4081 ccattcttcg atccccaggt tttggtaggt tttagccaga tggaacatgg catccccagc
     4141 agtttctaat tcaggaaatt ccttgatgat cttttcgaat cgatgggcag cagcgagata
     4201 agaaccacgt ttgaggtaga aatgcccaac catcaagtgc cgttgtgcta attgttcttt
     4261 acaaatcaga atggtttgct tcgcttccgt ttcatagcgg cttgccggaa attcttgaag
     4321 aagtttctcg aagctctgaa ttgattgctc caagggctct gggtcacgat cgactgtccg
     4381 gtattgtttg aaatggctga ccccgatttt gtattgggca taaggggcca atatgtgatt
     4441 tcggtgaagg tcaagaaaat gtttgtattc cacgatggat tccgcatagg attccttgtc
     4501 aaaaaaagat tcggcacgtt tcatgattac gttcggatcg taactcattt ctaccgaatc
     4561 accgacgaag atttgttcgt ccgttgagga cacttgttgt ttcgatgtgg tatcggcgtg
     4621 ctccgccggt ttgggtgttg aggagcatcc ggcgatcatg atgagagcag tgaggaaggg
     4681 aagcaaaagg gatcgaaaat tgaccggaaa tttcacaggt aagtcactcc aggcagtgtt
     4741 gtgatcatat attgcggtga aagtcggtat agaggtatga aacagcccat atctatacaa
     4801 gattcggacc aataaaagaa agtctttaat gttgcgactg tgttaatttt ttcattacaa
     4861 attgtctccc ttttaccaca gtatttggta gagatgaaaa atttgccaca gtcaatgggt
     4921 tgtcaattca gtcaattcaa tggataggct atgagaatta tatgaatgcc aacctttctc
     4981 gaatcgtcgg aaccggctct tttcttcctg gtcgtcaggt agataatcag gaggtggccg
     5041 atcttttggg aattgagccg tcgtatattt ttcgagtttc tgggatccgg gccaggtttt
     5101 gggcggattc gcatgaggaa tgttcatttt tggcggaaca ggcgacgcga agggcattgg
     5161 atcaggctgg tttggtcccg gaagatcttg acgctattct ggtttcttcc acctctccgg
     5221 agatgatctt tccctccacg gcctgtttgc ttcaagctcg gctgggcatc aggggcatcc
     5281 cggcatttga tctttcagcc tcttgctcag ggtttcttta tggcctttcc atggcggatt
     5341 gtttcattcg ggccgggcag tttcgaagat gtctcgtggt ggcttcggaa atcaaatctc
     5401 ggtccttgga tgtcaaagat ctgagtacca ccattttatt tggtgatggt gccggtgcgg
     5461 ctattgtcga gagatcgtca aattccgaca tgggcgttgt cagcattcga attcacgccg
     5521 acgggacgta tcatgatctg gtcaaaattg ccggaggtgg ttcccgtcag cctttgtctc
     5581 aagatgtcct tgatgccagt gcgcatacct tgcgaatacg gggttctcgg gtgtttcgca
     5641 tcgcaatcag gcgcttaggc caggcggtca gggaccattt ggcgcaggag aaatgggatg
     5701 tgtcgcagct cgaccaggtc attttccatc aagcgaatgg acgtatgctg acccagtttt
     5761 gtgaaaatat cggagtccct cctgagcgat gcctctcgat gatcgagcaa gtcggaaata
     5821 cctcttcggc ttcattgccg atggcgttag atcatgcgaa ccgaaatcaa aaactgaaga
     5881 cgggggatcg ggtcatgttg ggagcctttg ggggcgggct tacctggggc acggctttgg
     5941 ttcggtgggg ataacatcac cctctttgcg ttgtcgccgt gttatcggag aaatcgggtc
     6001 gggcattgtg ttgacgatct ctggcaattt agcctatcgt gcgcaaaaga catgcgtgga
     6061 atagataatt aagctagata aatattttgt aaggagggtg cgttgaaaat cttcctggcc
     6121 aatccaagag ggttctgtgc gggagtggat cgagcgatcg aaatcgtcga tttgtcctta
     6181 aagacttacg gggcaccaat ttatgttcgg catgaaattg tccatagtcg tcatgtggtc
     6241 gagtcccttc gtggaaaagg cgctattttt gttgaagaat tgaatgaggt tcctgatggc
     6301 gccatcgtca ttttcagtgc acatggcgtg gcaaaagagg tgtgggaaga atccagacga
     6361 cgaaatttga aagtaattga tgccacatgt cccctggtca tcaaggtcca taacgaagtg
     6421 aacagggatt attccaacga atatgagttg atcttgattg gtcatgcggg gcaccctgag
     6481 gttgtcggaa ctctcggaca ggtgcctgat aaattccatt tggtttcctc tgttgaggac
     6541 gtggactcgt tagaagtaga aaatcctctt cacctgtcgt atgtcaccca aaccacgttg
     6601 agcgtggatg aatgtcggga tattgttgct gccttgcatg gtcggttccc gggtattaag
     6661 gggccacatc aggaagatat ttgttatgcc acacaaaatc ggcaaaatgc ggtcaaagag
     6721 ctgtcccgtt ttgtggatgt cattctggta atcgggtctc ccaatagctc caattccaac
     6781 cgattgagag aattgggcga acgttgtgga atcccttcat acctcattga ttcttatcag
     6841 gatattcaga ctcaatggct ggaaggtgtg aacgcaatcg gcattgcggc aggggcctcg
     6901 gctccagaag tgttggtggc tcaggtcatt aagtttttga aagagcatgg agcgacatca
     6961 gtagaggagt tgacggtagt ggaagagaat gttgaatttc ttcttccaaa agaattactg
     7021 atggccaaag ttccgcgata agtttccaag gatttccgct ttcctggagg agcgcgagcg
     7081 tatcttttcc gaatttaccc ttcctcatta tctttcccaa tatattgaag ccgtcagacc
     7141 tgtttgcttg gcgaattctc ctggatggag aatgtcccgt gagaggctcc ttggttttgt
     7201 ccggttgaca ggagccgtag ttttggcaat atgtcaagat attgaatagg ccgatagtca
     7261 gacccacagt attttgtgga aagttttgat ttttctttct tcttatggta ctgatttgag
     7321 ccaaattact accctgtcat ccactccatt ctgagaggga atatgtatct tcgtacttta
     7381 gttgtcagtg ggtttaaatc ctttgctgag gcaaaaatag atttcccgaa cgggataacg
     7441 gctgttgtcg gtcccaacgg gacagggaaa agtaatatcg tggatgccat cttgtgggca
     7501 atgggtgagc aaagcgtgaa gagtttgcga agcgagcgca tggaagacgt aatttttaat
     7561 gggaccgagc aacgcaaacc catgggaatg gtcgaggctt cgctcatttt ttccgaggtg
     7621 acgccacggg agttggagcc tgtctctgcc atcctggaag ggcttgatca gccaacggat
     7681 gtgatgatca cccgtcggct ctaccgcgag ggaga
//
LOCUS       DATIAL010000433         4230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_939347, whole genome shotgun sequence.
ACCESSION   DATIAL010000433 DATIAL010000000
VERSION     DATIAL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939347"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VLA60_13395"
     CDS             complement(<1..690)
                     /locus_tag="VLA60_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HSF10404.1"
                     /translation="MSSPDQHVSIDFIRARVIADLENQTIGKRVHTRFPPEPNGHLHI
                     GHAKSICLNFGIAREFGGLCNLRFDDTNPTKENMEYVSSIQEDVRWLGFDWDDRLFFA
                     SDYFEQLYQFAVRLIQAGHAYVDSLTAEEIRVFRGTLTEPGKDSPYRNRPIEENLALF
                     TRMRSGEFEDGTHVLRAKIDMASPNINLRDPALYRIRHASHYRTGDDWCLYPTYDFAH
                     PLSDSLEGITHS"
     gene            complement(1339..2016)
                     /locus_tag="VLA60_13400"
     CDS             complement(1339..2016)
                     /locus_tag="VLA60_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10405.1"
                     /translation="MAKPVGKLRKINLSEVWGNEADGFASWLQQEEILDILGETIERP
                     LKPAGSDIPLSILAGGVLAKDAKTGSYVIVLGHLEGIDPDALGKLIMYAAGLDAKTVV
                     CVAQSISAEIRQTLDWLNGVSRDEVTFYGAELELWRIDDSVPAPNFHLVCQPNLWARQ
                     LKKGQDEGGESKAAKGSEFAESNANHEEGAKAPKEKGAAPQGKPAAVGKDGVSVRQNF
                     VYTKTFS"
     gene            2469..2735
                     /locus_tag="VLA60_13405"
     CDS             2469..2735
                     /locus_tag="VLA60_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10406.1"
                     /translation="MNRLSLLGLSLVCALGLMVSLSGCGETEGPAEKAGKTLDQAAEK
                     TTESVNDAMESTGEAMDNATEKTGEALNEAMESTEKMAEDTGKH"
     gene            3026..3556
                     /locus_tag="VLA60_13410"
     CDS             3026..3556
                     /locus_tag="VLA60_13410"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HSF10407.1"
                     /translation="MMIDKWIKTTLAVLALLFALSVGSPFARAENKWAVGTDIGFLSD
                     TTDDTVFTLSFQGDYFLNSQVSVGPQVLFSPGGDLTQVTFAGIGRYHIPVGAVTIIPF
                     GGIGFVYADLEHGRRDDSDVSYSFPFGATAAFKINRTVDLSSTLIFTFQDIELNNRKS
                     SDNFNIGMLFGFRFQP"
     gene            complement(3707..>4230)
                     /locus_tag="VLA60_13415"
     CDS             complement(3707..>4230)
                     /locus_tag="VLA60_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10408.1"
                     /translation="QFSGSRETFLIFIVVACGIMATLSVNLFNQSSSDPSEARVGEST
                     NPEQPKSPPAKVPSVELSDVPLATGTEPLEKLFVQSGCAVCHTIPGIAPALGREGPRL
                     VLGTNGPLRLADPQYQGTATTVREYVQESILNPGAYVVPGYSDRVMPRWYGKRLNAMA
                     LGRMAEYLENLKE"
BASE COUNT         1149 a         1127 c          983 g          971 t
ORIGIN      
        1 cgaatgcgta atgccctcta acgaatcgga aagaggatgc gcaaagtcat aggttggata
       61 gaggcaccag tcatcaccgg tccgatagtg cgaggcatgt cggatacgat acaaggcagg
      121 atctcgcaga ttaatattag gcgaggccat atcaattttg gctcgcagca catgggtccc
      181 gtcctcaaat tctccggacc gcattcgggt aaataaggcc aaattttctt caatgggacg
      241 attccggtag ggactatcct tccctggctc agtcagcgtc cctcgaaaaa cccggatctc
      301 ctcggccgtt aaactatcca cataggcatg cccggcttga atcagtcgca ccgcaaattg
      361 atagagctgc tcaaaatagt ctgaggcaaa aaagaggcga tcatcccaat caaagcccaa
      421 ccaccggaca tcttcctgaa ttgaagagac atattccatg ttctccttgg tcggattcgt
      481 atcatcgaaa cgaagattac acagcccacc gaattcccgg gcaatgccaa aattcaaaca
      541 gatggatttc gcatgaccaa tatgtaaatg cccattaggt tcagggggga aacgggtgtg
      601 cactcgtttg ccaatagtct ggttttccag atctgcaata accctggccc tgataaagtc
      661 gatggaaaca tgttggtctg gtgacgacat aatgaacaag ctttcaaaaa attaaatgaa
      721 aaaattttca gcaactcgca tcttcaaaaa caagactcgg actgtttaat tcccgtttct
      781 aggcatagag attgtccgaa aaggtccggg gacagccttt ccaaacaact tttccaaacg
      841 acctgtgggt tattcgccag gcgtgaagag tacagttgcc cccacgctac catgagtcgc
      901 gccaactctc aacacgggca agacaatatt cgatccttga gggttaccaa aacccgtcaa
      961 accggatcca gacagagaag gcatgagcgt cgcaactctt aagatgaaca ggcatgacgg
     1021 ctagagcgaa aatgaggatc cccatctgaa tctgccgtct tctcctggtt cggatttcag
     1081 gaaaaaatga ggaatccctt gtttatggca caggcgcaaa ttcaattcgg cttccggaga
     1141 tagattggca agccaccgcc cgggacctca tcgtttcata tggtggattc ggatcaagac
     1201 ccttgggtta gggtacggaa aaaatgccag ggaatgaaga gcactgcaac acccttcagg
     1261 agacccattc cctattacat gtattggaga tgaggtggat taaaaaacaa ccaacagaga
     1321 acgaaccgca accggatttt aggagaaggt tttggtatac acaaaattct gacgcacgga
     1381 gactccatct ttgcccacgg cagccggttt tccctgtgga gctgcgcctt tctcttttgg
     1441 cgctttcgcg ccttcttcat ggttcgcgtt cgattccgca aactcagatc ccttcgcagc
     1501 tttggattcc ccaccctcgt cctgaccctt tttcaattgc ctggcccaca ggttgggctg
     1561 acaaacaagg tgaaagttgg gagcagggac ggaatcatca atgcgccata actcaagttc
     1621 agcgccataa aatgtcactt catcacggga aacgccattc agccaatcca aggtttggcg
     1681 aatctccgca gaaatactct gggccacaca caccaccgtc ttcgcatcga ggcccgcagc
     1741 atacataatc agtttaccca gcgcgtcggg atcaatgccc tccaaatgac ccaacacaat
     1801 cacgtaactc ccggtcttcg catctttggc gaggacgcct ccggccaaaa tactgagagg
     1861 aatgtcactt ccagccggct tgaggggacg ctcgatcgtc tcccccaata tgtccaaaat
     1921 ttcctcctgc tgaagccatg aagcaaaccc gtcggcctca tttccccaca cttcacttaa
     1981 atttattttt cgaagctttc caactggttt tgccataaac aacctcaacc gcaaaaaatt
     2041 gttgttttct acttacttcc ccaatgagac gtactagtct tatcgacagg tattcaaaaa
     2101 agttaagcta caaaatacaa aaggcactat ctcccgatga accgtgtccg atcccgtcat
     2161 aaccgaccga caacagcaca acccgattcc gggaagacct cactcaaaac gtccgctgtc
     2221 ctgagcaatc cctggatttt tcgatttcaa tcaggtcatt cctcaggcaa gacattcacg
     2281 taaaaaaaat tacctactta tcattgatct ttcttcaaat tagagtacaa tagattaagt
     2341 tcattcacgc atggattggt cctcgtaccc cattcacccc cttctccaga ctcgaatacc
     2401 cctctggaac gagggaccaa aaggatggac agcaaggtgt tccccaactt taacaggagg
     2461 tttctgctat gaatcgatta tccctattag ggctgagtct cgtctgtgcg cttggactga
     2521 tggtttcact ctcagggtgt ggggaaacgg aaggtcctgc cgaaaaagct gggaaaaccc
     2581 ttgatcaagc ggccgaaaaa acaaccgaat ccgtcaatga cgctatggaa agcacagggg
     2641 aagccatgga caacgcgacc gaaaaaaccg gagaagccct caacgaggca atggaaagta
     2701 ccgagaaaat ggcagaggat accgggaaac attaaagaaa atttcctgaa ctcacgtatc
     2761 tgagagtccc tgacaccctt ccgaattttc cagcttttgc tggcaacagc cgggaggaaa
     2821 tgggcaaagg gtctcatctt ccttttttct tgggccttca ggtggtcgat ggcataccat
     2881 caggatccag ggaagagggg agttgtgaaa ccgaggttaa gcaaaaacgg cggggtcagc
     2941 actaaagacc aggaagtgat tcgaaataag gaaagggtaa gtcccatcgc tcacacaacc
     3001 gattgaacca agaggaaaac gagacatgat gatcgataag tggataaaaa cgaccttggc
     3061 cgtcttagcc ctcctttttg cgctctcagt gggcagtcct ttcgcacggg cggaaaacaa
     3121 gtgggcggtt ggaacggata tcgggtttct gtcagatacc accgacgaca ccgtgttcac
     3181 gctcagtttc cagggagact attttctcaa ctcacaagtc tctgtgggcc cgcaggtctt
     3241 gttttctccc ggcggcgacc tcactcaagt caccttcgcc ggaatcggcc ggtatcacat
     3301 tcccgtcggc gctgtcacca tcattccttt tgggggaatt ggctttgtct atgccgattt
     3361 ggaacacggc cgtcgtgatg acagcgatgt cagttatagc tttccttttg gggctactgc
     3421 agccttcaag atcaatcgga ccgttgatct gtcaagtaca ctcatattta ccttccagga
     3481 cattgaactg aataatcgga aaagcagcga taatttcaac atcgggatgt tattcggctt
     3541 tcggttccaa ccctaatacc ctcacatcac ccttcacgat ggcaggcggt cgtactgttg
     3601 gatgaccatc accgacggaa ccgacccgtt caaaggggtt gggagagcgt accccaaaaa
     3661 tagacgaata gatgcgttca cgtcgcgaga agctgcccga ggctttttac tccttcaggt
     3721 tttctaaata ctcagccatt cgacccaacg ccatcgcatt gagccgcttc ccataccatc
     3781 ggggcatcac ccgatcagag tatccgggca ccacgtatgc gcctggattc aaaatcgatt
     3841 cctggacata ttcacgaacg gttgtcgccg tcccctgata ttgagggtcc gctaatcgaa
     3901 gcggtccatt cgtccctagg actaaccgag gaccctcacg ccccaaagca ggagcaatac
     3961 caggaatggt atgacacacc gcgcagccgg attgcacaaa caacttttcc agcggctcgg
     4021 tgcccgtggc caatggcaca tcgctcagct ccacagacgg aactttggcg ggtggggact
     4081 ttggttgctc cggattggtg gattccccca cgcgggcttc gctaggatcc gaagacgatt
     4141 gattgaacag attcacagaa agggtcgcca taatcccgca ggccaccaca ataaaaatga
     4201 gaaaggtttc ccggcttcct gaaaattgtg
//
LOCUS       DATIAL010000434         7034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_186979, whole genome shotgun sequence.
ACCESSION   DATIAL010000434 DATIAL010000000
VERSION     DATIAL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186979"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4480)
                     /gene="gltB"
                     /locus_tag="VLA60_13420"
     CDS             complement(<1..4480)
                     /gene="gltB"
                     /locus_tag="VLA60_13420"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HSF10409.1"
                     /translation="MKQVTNQPVWNLPGLPSRQGLYNPGNEHDGCGIGFVAHIKGQKS
                     HDIIEKGLEVNKNLTHRGAQGCDPCTGDGAGILSQIPHEFFHRVAAETGVDLPEAGAY
                     GVGMVFLPQDARTRQQCEAIFEAIVHEEGQYFLGWRDVPAKEDQIGDQARKTMPVIRQ
                     FFVARELLNPMQFERKLYVIRKRITRAIRESALPGKEWVYISSLSGNTIVYKGMLLPE
                     QVALFYPDLADPTFTSALALVHSRFSTNTFPTWALAHPYRYSVHNGEINTLKGNVNWM
                     RARQGRLASDLFGDDLKKLFPIIDDSTQSDSACLDNAIEFLVMAGRSLPHAMMMLIPE
                     PWVGNPQMDFDRRGFYEYHAAVMEPWDGPAAVCFTDGKLVGATLDRNGLRPCRYQITK
                     DDVVVLASEAGVLPTDPKTIRVKGRLQPGRMFVVDTVQGRILDDEEIKADITKRKPYR
                     QWLTQYRVSLDELPEPLNVPQPDHPTLRQRQQAFGYTVEELKMVLIPMAVTGEEPISS
                     MGTDTPLAVLSERPQLLFKYFKQLFAQVTNPPIDPIREHLVMSLVTNIGPKPNVIAEI
                     PEACRRIKLQQPILSNVDLQKIRMIGDPHFKSKTLSLLFKVAEGPEGMAAALDQLCQQ
                     ASKAIKDGEKFLILSDRGVNAEWAPIPSLLGVSAVHHHLVREETRTEVGLILETGEPR
                     DVHHFACLIGYGAGAINPYLVFESLVDMEREGYFPESVDAATAESKFIKAVNKGLLKI
                     FSKMGISTVQSYCGAQIFEAIGLNNKLIDRFFTGTASRIEGIGLSEVGEETLRRHALA
                     YRPVPMRQLDFGGEIHYRIQGEHHNWNPETIYKLQHASRANDAKAYADFSALVNKEDQ
                     RRSNLRGLFEFNWAAEPVPLEEVEPASEIVKRFTTGAMSFGAISKEAHETLAIAMNRI
                     GAKSNTGEGGEDPERFTPLPNGDSKNSYIKQVASGRFGVTAHYLVNAKELQIKMAQGA
                     KPGEGGQLPGHKVDEFIAKLRYSTPGVQLISPPPHHDIYSIEDLKQLIFDLKNSNPEA
                     AISVKLVAEVGVGTVAAGVAKAHADKVLISGDSGGTGASPLSSIKYAGVPWELGLAET
                     HQTLVLNNLRGRIRVETDGQLKTGRDVVIATLLGAEEFGFSTAPLIVEGCIMMRKCHL
                     NTCPVGVATQDGELRKQFTGKPEHVVNFFMFVAEEIRSLMAKLGFRTMREMIGRVDKL
                     KVQKAVDHWKAKGLDLSPLLVKPNVGPEVATSCVQAQDHGLKGILDNQLVELCQSAID
                     RGESVSLDLPIRNVNRTTGTVLSSHIARRYGPEGLPPDTIRIKFTGSAGQSFGAFLSK
                     GITLTLEGESNDYLGKGLSGGTIIVVPPKGVTYLPEDTILIGNTSLYGATGGEGYFYG
                     IAGERFAVRNSGARAVIDGTGDHGCEYMTGGVVVVLGKTGRNFAAGMSGGDAYVLNED
                     GQFPARCNMGMVELEPVVSSEDQQTLRAMIEAHFRYTQSVNARRVLESWDMMLPKFV"
     gene            4784..5788
                     /locus_tag="VLA60_13425"
     CDS             4784..5788
                     /locus_tag="VLA60_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HSF10410.1"
                     /translation="MHQEPALRDLESMEDVWEAFRPDLIEVESQITTHLESHAPLINT
                     VAGHIFASGGKRIRPLLLILCARLCGFLQKDFLLLGSLVEFIHTATLLHDDVLDEADL
                     RRGQPTARKIWGNQASILVGDYLYSQAMALIATFHNHGINEALADACRKMAEGEVLQL
                     CANSQPHLSEAAYLKIVEYKTAALVAASCKVGAIVGGASIEEQAALYRFGYHLGMAFQ
                     LADDRLDYSADRAKLGKALGQDIRQGMITIPLLHLLQTCSPQDNQWITEKILAHAIED
                     EDVTRIIQLMQKQGSLSYSTARAREYVEAAALDLEPFPACMAKRSLSITACYMVNRDQ
                     "
     gene            6132..6578
                     /locus_tag="VLA60_13430"
     CDS             6132..6578
                     /locus_tag="VLA60_13430"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HSF10411.1"
                     /translation="MTREEQTLQRIQRQLATLGPVLPGSLSEQWNVCGTPGCRCKAPS
                     NPKKHGPYYQLSFTVAGKSSTLFVGSGRGATAAQALSAIQGVNHGLGPGVSGPGSQPG
                     LQGGDRMRGLPRLFAGLKKTRLLDWPNTRGSSNATGGSRLMTFSCS"
     gene            6569..>7034
                     /locus_tag="VLA60_13435"
     CDS             6569..>7034
                     /locus_tag="VLA60_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSF10412.1"
                     /translation="MLLTLGHLGLAHPSLGGMVAALSSRISREALHQRFTASAAAFLR
                     HCLQTVLQYKLQTVPIRTNLLQPFARVLLVASSSWDVSEKLGAVLPGSGGNASIANCK
                     LQTAYDYKQGELTFLADTAGTVPDNRYTDHLPALANRTDLVLFDQGYFKLTTV"
BASE COUNT         1687 a         1962 c         1681 g         1704 t
ORIGIN      
        1 tgacaaattt cggcaacatc atatcccacg attccagaac ccgcctggca ttgacacttt
       61 gggtgtagcg gaaatgggcc tcaatcatcg cacgaagggt ctgttgatct tcggatgaca
      121 cgacaggctc taattccacc atgcccatat tgcaacgtgc agggaattgt ccatcctcat
      181 tcaagacata ggcatcccct ccggacatcc cagcggcaaa atttcgtccc gtcttcccca
      241 gaaccaccac cacccccccg gtcatatatt cacacccatg atcgccggtt ccatcgatga
      301 cagcccgtgc gccactattc cgaacagcaa accgttctcc cgcaatgcca taaaaatacc
      361 cttcaccacc agtcgcccca tataaagacg tgttcccgat cagaatcgtg tcttccggca
      421 aatacgtcac ccctttcggg ggaacgacaa taattgtccc tccagagaga ccctttccca
      481 aatagtcatt cgactcacct tccagagtca gggtaatacc cttcgacaaa aatgctccaa
      541 aggattgtcc ggccgatccg gtaaatttaa tccgaatcgt gtcgggagga agcccttccg
      601 gcccataccg gcgtgcaata tggcttgaaa gaacggtacc ggtggtccgg ttcacatttc
      661 gtataggcag atccagcgaa acagattcgc cccggtcaat ggcagattga cacaattcca
      721 ccaattgatt atccaaaatt cctttcagac cgtgatcttg tgcttgcaca cacgaggtgg
      781 ccacttcagg gccgacattc ggcttcacca ataaggggga taaatccaat cctttggctt
      841 tccaatgatc cacagctttt tgcactttga gcttatccac acgtccgatc atctcgcgca
      901 tggtcctgaa tccgagtttc gccatcaggg aacggatctc ttctgccaca aacatgaaga
      961 agttcacaac atgctctggt tttcccgtaa attgttttcg taactcccca tcttgggtgg
     1021 caactcccac cggacaggta ttgaggtggc atttcctcat catgatgcat ccctcaacaa
     1081 tgaggggggc tgtggaaaaa ccaaactctt ccgctcccaa aagggttgca atgaccacat
     1141 cacggccagt tttcagttgg ccgtcggttt ccactcgaat tcgtccacgc aggttattga
     1201 gcacgagcgt ctgatgggtt tccgccaggc ctaactccca ggggactcct gcgtacttaa
     1261 tggacgatag cggagacgct cccgttcctc cggaatctcc acttatgaga actttatcgg
     1321 catgagcttt tgccacgcct gccgccacgg ttccaactcc gacttcggcc acgagtttca
     1381 cggaaatcgc ggcttcagga ttggaatttt tgaggtcgaa aatgagttgt ttcagatctt
     1441 cgatagaata aatatcatga tgaggggggg gagagatcag ttgcactccc ggcgtggaat
     1501 agcgaagttt agcaataaat tcatcgactt tgtgtcccgg caattgcccg ccttcgcctg
     1561 gcttggcacc ctgggccatt ttaatttgta actccttggc attcaccagg taatgtgccg
     1621 tcaccccaaa acgacccgat gccacttgct taatataaga atttttggaa tcgccgttcg
     1681 gcaaaggtgt aaatcgttcc ggatcctcac caccttcgcc ggtattgctt ttggcgccaa
     1741 tgcgattcat ggcaatggcc aaagtctcat gcgcttcttt actgattgca ccaaacgaca
     1801 tggcaccggt agtaaaccgc ttcacaattt cgctggcggg ttccacctct tcaagaggga
     1861 ccggttcggc cgcccagtta aactcgaaca gtccgcgaag attcgagcgg cgttgatcct
     1921 ctttattcac cagggccgaa aaatccgcat aggctttggc atcattcgca cgcgaggcat
     1981 gctgaagttt gtaaatggtt tcaggattcc aattatgatg ctctccctga atccggtaat
     2041 gaatttcgcc accaaaatct agttgccgca tggggaccgg acgataggcc agcgcatgtc
     2101 tgcgcagggt ctcttccccc acctcactga gaccaattcc ttcgatccgt gaagccgttc
     2161 ccgtaaagaa ccgatcaatc agtttgttat tcaggccaat ggcctcaaaa atttgagctc
     2221 cacaatagga ctggactgtg gagatcccca tctttgagaa tatcttcagc aatcctttat
     2281 taactgcctt aatgaacttt gattcagccg tggccgcatc cacgctttca ggaaaatacc
     2341 cttcccgttc catatccacg agggactcaa acaccaaata aggattgatg gccccggcgc
     2401 cataaccgat gagacaggcg aaatgatgca catcacgcgg ttcacctgtt tccagaatca
     2461 accccacctc cgtccgggtt tcttctcgaa ccagatgatg atgcaccgcc gaaacgccta
     2521 atagactagg aatgggcgcc cactcggcat tgacacctcg atcactcaaa atgagaaact
     2581 tttccccatc ctttatggct ttcgaggctt gctggcatag ctgatcaaga gcggcagcca
     2641 tgccctcagg cccttcagca accttaaaaa gcaaggacaa tgtcttactt ttaaagtgtg
     2701 gatccccaat catgcgaatc ttttgcaggt cgacattgct aaggattggt tgctgcaatt
     2761 tgatccttcg gcatgcctct ggaatctctg caatgacatt cggttttggc ccgatgttcg
     2821 tgacaagaga catcaccaga tgctcacgga tcgggtcgat gggaggattc gtcacttgcg
     2881 caaacaattg tttgaagtat ttaaaaagaa gttgcggtcg ttcggacaac acggccaacg
     2941 gggtatcggt tcccattgaa gaaataggct cttcacccgt gacggccata gggatcaaca
     3001 ccattttcaa ctcttccacg gtgtacccga aggcttgttg acgttggcgc agtgtcgggt
     3061 ggtcgggttg tggcacattg agtggctcag gcagttcatc taaggaaacg cgatattgcg
     3121 ttaaccattg tcgatagggc ttacgtttgg tgatatcggc tttaatttcc tcgtcgtcca
     3181 gaatacgtcc ttggacggta tccacaacaa acatgcgccc tggttgcaga cgtcccttca
     3241 cgcgaatggt cttaggatca gtcggaagta ctcccgcctc tgaagccaac accacgacat
     3301 catccttggt gatttgatag cgacagggac gcagaccatt ccgatccaag gtggcaccca
     3361 cgagctttcc atccgtaaag cacacagccg cgggtccatc ccacggttcc atcaccgccg
     3421 cgtgatattc ataaaaccct cgccggtcaa aatccatttg tggatttccc acccacggtt
     3481 ccggaatcag catcatcatg gcatggggca aggaccggcc ggccatcacc agaaattcta
     3541 tggcgttatc caaacaggcc gaatcgcttt gtgtggaatc atcaatgatg ggaaataatt
     3601 ttttgagatc atccccgaac aggtcagagg caagcctgcc ctgtcgtgcc cgcatccaat
     3661 tgacattccc tttcaaagta ttaatttcgc cattatggac ggaatagcgg tagggatggg
     3721 ctaaggccca ggtcggaaac gtgtttgtac taaagcgaga atgcacgagg gccagggccg
     3781 acgtaaatgt gggatccgca agatcggggt aaaacagtgc aacctgttcc ggcaacagca
     3841 tccccttata gacaatcgta ttcccggaca gactggatat atagacccat tcctttccgg
     3901 ggagagccga ttcgcgaata gcacgggtta ttcgtttacg aatgacatac agtttgcgtt
     3961 caaactgcat gggattcagt aattcacgtg ccacaaaaaa ctgtcgaatg acgggcatcg
     4021 tttttcgggc ttgatccccg atttgatcct ctttggccgg cacatcccgc caccccaaga
     4081 aatattgccc ctcttcatgt acgatcgctt caaagatggc ttcgcactgt tgcctggtcc
     4141 tggcatcctg cggcaaaaac accatgccga ccccataggc tccggcttca ggaaggtcga
     4201 cacccgtttc ggccgcaacc cgatgaaaaa actcgtgggg gatttgagag agtattcctg
     4261 ctccatcacc ggtacatgga tcacaccctt gtgctccgcg atgagtgaga tttttgttga
     4321 cctccaggcc cttttctatg atgtcatgtg atttttggcc ctttatatgg gccacaaacc
     4381 cgattccaca tccatcatgc tcatttccgg gattgtagag gccttgcctt gaaggcaatc
     4441 ccgggagatt ccacactggt tgattagtca cctgtttcac gtttcaccta aaatattgtt
     4501 ggccatcgtt aaaaaggaga ttaaggcacg ctacccctta atagagtctc tatgggccat
     4561 ggtgtcgttg tgtggtcccg gagagcgacg gaagaaaagg gaagtctaaa tcaaaattgt
     4621 aaacgacaag attaatggat ggtcaaaaaa tctgtcaaga cgatcaattt tccgctccgc
     4681 aatatttccc tcacatgtat gcgggttttc gaaaaaatgc tgattgattt ccaagctatg
     4741 gttcagcttg acaatccaca gacgaccccc tagtatcgcc gccatgcatc aggaaccagc
     4801 ccttagagac ctggagtcca tggaggatgt gtgggaagcc tttcggcctg acctcattga
     4861 ggttgaaagc caaattacca cacaccttga atcgcatgcc ccactcatca ataccgtcgc
     4921 tgggcacatc tttgccagcg gaggaaaacg tatccgtccc ttgctcctga ttctctgcgc
     4981 ccgactctgt ggatttcttc aaaaagattt tttgttgctg ggcagtctcg ttgaatttat
     5041 ccatacggcc acgttactac atgacgatgt gttagacgaa gccgatctta gacggggaca
     5101 acccactgcg agaaaaatct ggggcaatca agccagtatt ctggttgggg attatttata
     5161 ttcccaggcc atggccctga ttgccacctt tcacaaccat ggcatcaacg aagccttagc
     5221 ggatgcgtgc cgaaagatgg cagaagggga ggtccttcaa ctctgcgcca acagtcaacc
     5281 gcacctgtcg gaagccgcct atctcaaaat tgttgaatat aaaacggctg ccctggtcgc
     5341 tgcctcttgt aaggtcgggg cgatcgtggg aggtgcctcg atagaagaac aagccgcgct
     5401 ctatcggttt ggctaccatc ttggaatggc attccaattg gctgacgatc gcttggatta
     5461 ttccgcagac cgtgcaaaac tcggcaaagc ccttggtcag gatattcgcc agggcatgat
     5521 taccattccc ctccttcatc ttctccagac ttgttcccct caagacaacc aatggatcac
     5581 cgaaaaaatc ttggcacatg ccatagagga tgaggacgtc acgagaatca tccagttaat
     5641 gcagaaacaa ggctctctgt cctattccac tgctcgagcg cgagaatacg tagaagccgc
     5701 ggccctcgac cttgaaccgt tcccggcctg catggccaaa cgatccctct ccatcaccgc
     5761 ctgctatatg gtcaaccgcg accaataata ccctccaaaa attattcgta attccctttt
     5821 cgaagtataa gaatcgagaa ttggctcatt cttcgcctaa gccgatccta cacctcctgt
     5881 cattccggcc agtttttagc gggaacccat tttagctttt gaaaatcacc aaaacgttgt
     5941 tttaagacaa cgcgtcttac tcgtttatca aatgaccatg tccaagcgat ttttgatgtt
     6001 gccgggtctc gccccatagc tgttaagcta agcaaagatg ttccttgtcc ctgcgcttgt
     6061 cttgcgcttt ttttgtgtta tattaccgat agctttatta aagctatcgg ccataacgga
     6121 aggaggaacc gatgacgcga gaagagcaga ccctgcaacg aattcagcgg cagctggcca
     6181 ccttgggccc ggtcttaccg gggagtctaa gcgaacagtg gaatgtgtgc gggacgcccg
     6241 gctgtcggtg caaagctccc tccaatccca agaagcatgg gccctactat cagctcagtt
     6301 ttaccgtggc gggcaagagt tccacgctct tcgtgggatc tgggcgaggt gcgacggcgg
     6361 ctcaagcgct atcagcgatt caaggcgtta accacggact gggtccaggc gtgtctggcc
     6421 ctggctcgca gccagggctt caggggggcg accgcatgag ggggctgccg aggttgtttg
     6481 cggggttaaa aaaaacgaga ttactcgact ggccaaacac acggggttcc agcaacgcca
     6541 cgggcggctc acgcctcatg actttctcgt gctcctgacc ctgggccacc tggggctggc
     6601 gcatccgtcg ttggggggga tggtagcggc gctctcctcg cgcatcagtc gcgaagcctt
     6661 acatcaacgc tttacggcct cggcggccgc ctttctccgc cactgtctgc agacggtgct
     6721 tcaatacaaa ctgcagacgg tgccgatccg cacgaacctg ctccagccgt ttgcccgcgt
     6781 cctgctcgtg gctagctcga gttgggatgt cagcgagaaa ttgggtgccg tccttcccgg
     6841 ctcagggggc aacgcctcca tcgccaactg caaacttcaa acggcgtatg actacaagca
     6901 gggcgaactg acctttctgg cggacaccgc tggcaccgtg ccggataatc gctacacgga
     6961 ccatctgcct gccctggcca acaggaccga cctggtgctc ttcgatcagg gctatttcaa
     7021 gctgacgacc gtca
//
LOCUS       DATIAL010000435        15354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_79434, whole genome shotgun sequence.
ACCESSION   DATIAL010000435 DATIAL010000000
VERSION     DATIAL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15354
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79434"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..632
                     /locus_tag="VLA60_13440"
     CDS             285..632
                     /locus_tag="VLA60_13440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF10413.1"
                     /translation="MESRPIIDRAIVAKNWHARGFSCGVWIDHAGREWRAATQDVEEI
                     FMAMSGELELEIAGERVQTSAGKEVLIPSGAPYTIRNIGGTTARWLYGKKRSPSAPLQ
                     PVESNRDLIHTPL"
     gene            complement(674..1540)
                     /locus_tag="VLA60_13445"
     CDS             complement(674..1540)
                     /locus_tag="VLA60_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF10414.1"
                     /translation="MTHTWLLGILAVVFIFNGLSQPTLAQESQGGNSWLDAPVQDDGP
                     PGSMVLIPEGEFLMGDNDGPRNERPEHTVWLDAYSIDRYEVTMAEYQKLLDTDYSIEP
                     PPLWDDGVATGELGIRPAVGISWNDANRYCQWVEKRLPTEAEWEKAARGTDGRRYPWG
                     HMQPFVDIANYNQGTTGWVSYKLTLSPVTSGTRGMSIRHGLKQGGKSAYGLYHMAGNA
                     AEWVNDWYERFYYEESPKKNPQGPAEGDYKVLRGGSWEDQPVNLRVTARSKGEVDFQD
                     LTTGFRCAKDAK"
     gene            complement(1959..2036)
                     /locus_tag="VLA60_13450"
     tRNA            complement(1959..2036)
                     /locus_tag="VLA60_13450"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1998..2000),aa:Glu,seq:ttc)
     gene            complement(2292..2716)
                     /gene="rnpB"
                     /locus_tag="VLA60_13455"
     ncRNA           complement(2292..2716)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VLA60_13455"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2784..3512)
                     /locus_tag="VLA60_13460"
     CDS             complement(2784..3512)
                     /locus_tag="VLA60_13460"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HSF10415.1"
                     /translation="MNLQLQYLINLQKFDLRIFQIQDQLRKAPELLKSAETPLQDILA
                     KLQVLKNTGESLVKQRRSSERELATQEEQLQKIRNRLSELKTNKEYQAHLFEIELARK
                     KKDSIEENVLEIMERVEQNEQALKELEEQSIEAQKVFDSEKARLETHFANLANELADL
                     DRQQTTLSLQVDKPLLVRYNRLKTLRKGYAVAQLRDGACGGCQLQLPPQLVAEVKRGD
                     ELLDCSYCHRILYMARHLEEETTE"
     gene            complement(3574..3650)
                     /locus_tag="VLA60_13465"
     tRNA            complement(3574..3650)
                     /locus_tag="VLA60_13465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3614..3616),aa:Ile,seq:cat)
     gene            complement(3742..5568)
                     /gene="dnaG"
                     /locus_tag="VLA60_13470"
     CDS             complement(3742..5568)
                     /gene="dnaG"
                     /locus_tag="VLA60_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HSF10416.1"
                     /translation="MTGEKRGVPPEILQQIRDRIDIIDLISTYVSLSKAGQNFKGLCP
                     FHSEKTPSFSVNPVRQMFYCFGCSVGGDAFTFLMKQEGMDFMEALRELSQRTGVPLPE
                     RRESAGKTTSGLSRERYFHLYQLAASWYHRNLQNNPEGQVARDYLDQRGITRESWNTF
                     QLGFVPEGWNGLSKWLEQQSVKPEELIQAGLVVRKESEDGTRISTYDRFRDRVMFPIT
                     DPRGQVVGFGGRILKDGASPKYLNSPETDLFFKGRSLYGMDKARQSATASGRFYLVEG
                     YFDVIALHQNGIENAVAPLGTALTSDHVQILRRLAPSVMLVFDGDAAGIGAALRTLDL
                     FMNSGIEVRVLLLPAGEDPDTFIRKNGVSAFRELEGKAATLLDFAIMSVLDKAKNDSI
                     QDRVKRADEILAILHKTKNPIEKEEYLKVVSERLGIRPELLRKRLPTISRPMSVAPSA
                     KGKEQAATNLAIPQGKPEERDLIVLLLQGRLEPDQIRQLDEGAFTVPLYRHILGKAFQ
                     HSDDEGNLNLDALHAEFSQDPAYEPVISRLSVGEYNCDEVSIHVAGCLRVLKKNQIQR
                     LMDEVINQLKVAQREGRQDLVDALVAESNVLRQKKALLTAST"
     gene            complement(5668..6012)
                     /locus_tag="VLA60_13475"
     CDS             complement(5668..6012)
                     /locus_tag="VLA60_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HSF10417.1"
                     /translation="MGDCLFCQIVKGAIPADTLYEDEHTVVFNDINPQAQVHMLVIPK
                     RHVVSLDDTKETDAALLGHLMVVCAKMARQRDIVGGGYRVVANIGPGAGQSVFHLHFH
                     VMGGRTFAWPPG"
     gene            complement(6005..6700)
                     /gene="hisIE"
                     /locus_tag="VLA60_13480"
     CDS             complement(6005..6700)
                     /gene="hisIE"
                     /locus_tag="VLA60_13480"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HSF10418.1"
                     /translation="MSEVSSTSTLQFDEQGLLPCVVQDWLDGTVLMVGFMNQQAWEMT
                     CDSQRVHFWSRSRKRLWKKGETSGHELLLKEMFVDCDRDTILVKAQPIGPTCHTGARS
                     CFFSPLPSSTSEEPAAFFSGTAWGGITRRLYEMVGDRKANPSPDSYVSKLMEEGLDRI
                     LKKVAEEAGEVLLAGKNGDREEIIYEMADLWFHTLIMLGHFSIPPEDVYQELGKRFGK
                     SGIRQPEGGSPHG"
     gene            complement(6707..7504)
                     /gene="hisF"
                     /locus_tag="VLA60_13485"
     CDS             complement(6707..7504)
                     /gene="hisF"
                     /locus_tag="VLA60_13485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HSF10419.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGVSFVNLRDAGDPVHIAQVYDREGA
                     DELCFLDITASHEKRDTILDVVIRTAEQVFMPLTVGGGIRTIEDIRRLLEAGADKVSI
                     NTAAVEHPDFVKAAANRFGSQCIVVAIDAKRATTCEGGEPGWEVFTHGGRNSTGLDAV
                     EWGKRMKDYGAGEILLTSMDQDGKQDGYDLALTAAISESVSIPVIASGGAGTLQHLYE
                     AFHVGKADAVLAASIFHYQTFSIGQAKSYLAQQGIPVRPGTVPVSIS"
     gene            complement(7510..8244)
                     /gene="hisA"
                     /locus_tag="VLA60_13490"
     CDS             complement(7510..8244)
                     /gene="hisA"
                     /locus_tag="VLA60_13490"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HSF10420.1"
                     /translation="MLLIPAIDLKDGKCVRLRQGEMDQVTRYSDNPAAMAEHWQSLGA
                     RYLHVVDLDGAVTGTPQHLSHIEAMTKRLAIPVQVGGGIRNTSTIRAYLSCGVDRVVI
                     GTAALQDADFLAAAATEFPDKILIGIDVKQGLVAVHGWKTVSSLTPRQVFESVKDLPL
                     GGMVFTDISRDGMLAGPNISALREAVDISPFPVIASGGVTVLKDVQTIQALGSRISGI
                     IIGKALYEGTLDLKAALEMVALAEAS"
     gene            complement(8248..9051)
                     /locus_tag="VLA60_13495"
     CDS             complement(8248..9051)
                     /locus_tag="VLA60_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10421.1"
                     /translation="MCFAINGRSRPMPSRFLVMSLIGWWCLGVGCVPPKVQIQAAPQF
                     DPSRISSLAILPFQTIKTPQLASSSTRGGIRDPEEIRTQFRLPGTDQADGTESRKVRY
                     VVPETAAHRITTQVASVLAGRPALRVIGPEESSAVMGKESPEGESSLPVMAQEVGTRL
                     KVDGVVGGLVRTYREREGSKLGAKPAAVGFEVYLLRPSDGMVLWTGEFFEEQKPLNQD
                     VVGFFEKGGGFVTADKLAELGVNKVMKAFPVGLGEQGPPLPAPSLKENP"
     gene            complement(9057..9680)
                     /gene="hisH"
                     /locus_tag="VLA60_13500"
     CDS             complement(9057..9680)
                     /gene="hisH"
                     /locus_tag="VLA60_13500"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HSF10422.1"
                     /translation="MIAIIDYGMGNLRSVQKGFECVGHDAVVTRDLLVIDQASHVVLP
                     GVGAFGDCMDNLARFELMDVIYRSIASGKPFLGICLGYQLLFSESEEFGCHQGLGILA
                     GKVKAIPRPSDDGATSFKIPHMGWNTIQVNTVAPPLRGIASDSYVYFVHSYYVEPTDC
                     ALVSTQTDYGISFASSVWKDNVFATQWHPEKSQAVGLQVLKNFGDWQ"
     gene            complement(9725..10327)
                     /gene="hisB"
                     /locus_tag="VLA60_13505"
     CDS             complement(9725..10327)
                     /gene="hisB"
                     /locus_tag="VLA60_13505"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HSF10423.1"
                     /translation="MNRHLVPRTASQERKTAETAIRVEWGLDGCGNNQIKTSIPFLDH
                     MLNLFGKHGFFDLTVEAKGDIEIDDHHTIEDVGIVMGQVLTQALGTKEGLTRFGWATV
                     PLDETLAQVTVDLSGRPYLVYNVVLPTRYIKSFDLGLFEDFFQAFVSQGGLNLHVNVL
                     YGRNPHHIMEAIFKAFAKAMDQAVRREERIKGVLSTKGLL"
     gene            complement(10324..11607)
                     /gene="hisD"
                     /locus_tag="VLA60_13510"
     CDS             complement(10324..11607)
                     /gene="hisD"
                     /locus_tag="VLA60_13510"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HSF10424.1"
                     /translation="MKIVSHKDQEFSQAVNRVCQRAAQQNDKVEKAVRTILDAVRQEG
                     DQALQRLTWKFDHVRISPDGLRVTSKDVERAYAELPSSDIKALKYAANRIRTFHRRQR
                     RKSWVYRQQGIQLGQRITPLDAVGVYVPGGKALYPSSVLMNAIPAKVAGVKRVVMCTP
                     TPNGIIHPALLAAADVAGVNEIYRVGGAQAIGALAFGTSTIRPVDKIVGPGNMYVATA
                     KRLVYGQVDIDMIAGPSELLVIADSASNPLHVAADLLCEAEHDEEACVYLVTTSLTLA
                     RRVVTEVTRQLAQLGRQAIASRSIHNHALAFVVRTFDEAFELANRIAPEHLSLNVDRA
                     GDYVSRIRHAGAIFVGRYTPPAVVDYVAGPNHVLPTGGSARFFSCLGVDEYMKTSNVV
                     AYSKAALRKAHAPVMRLSLLEGLDAHGRSMESRWT"
     gene            complement(11604..12266)
                     /gene="hisG"
                     /locus_tag="VLA60_13515"
     CDS             complement(11604..12266)
                     /gene="hisG"
                     /locus_tag="VLA60_13515"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HSF10425.1"
                     /translation="MSEGITIALSKGKLLDPTLELFRQAGYTGYHVRADDRRLILEFP
                     EHGHRVLIVRPSDVPAYVEYGAADLGVVGKDVLLEQSPDVYEPVDLNLGVCRLVVARP
                     NGPQLVQRMSSKLRVATKYPMITERFFNQQGLPVELIKLYGSIELAPLVGLADRIVDL
                     VETGNTLKANNLVEDEVIAWSSARLIVNRASLKMKHAVITELIARVKKILSRSRSKKV
                     HA"
     gene            complement(12263..13522)
                     /gene="murA"
                     /locus_tag="VLA60_13520"
     CDS             complement(12263..13522)
                     /gene="murA"
                     /locus_tag="VLA60_13520"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HSF10426.1"
                     /translation="MDQIRITGGLPLKGSVEIGGAKNAALPILAATLLGGGECVIDHV
                     PHVRDVMTMGKLLALLGVAVQEEGARVRLDAAQVHSIEAPYDLVKTMRASILALGPLL
                     ARLGEAKVSLPGGCAIGTRPVNLHLKGLEMMGAEIQVEHGYIHAKAGRLKGARIDLDF
                     PTVTGTENLIMAACLAQGTTSLHNVAREPEITDLCAFLTKRGAKIHGMGTDTITIEGV
                     KELHGAGHWVIPDRVEAGTYLMAGAITGGDVMVKGCVTDHLGSVLKKVQEMGAELTES
                     HDGVRIRRSGPLKAVEIKTLPYPGFPTDLQAQMMALMSVAEGVSVISETIFEGRFLHV
                     PELHRMGASIEIDGPHAVVKGVPLLTGAPVMASDLRASAGLVLAGLAAVGETIVSRVY
                     HLDRGYERLEEKFQALGARIVRVREVT"
     gene            complement(13539..14417)
                     /gene="prmC"
                     /locus_tag="VLA60_13525"
     CDS             complement(13539..14417)
                     /gene="prmC"
                     /locus_tag="VLA60_13525"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HSF10427.1"
                     /translation="MPPFTSYSQLYRGAVETLIHAGIANARHEALWILEDALGITRLQ
                     LHANPDTPVDAEVCRQAVALFQRRALHEPLQYVLGNQDFFGLDFLVRPGVLIPRPETE
                     LLVEEAIGLLGADACPVILDVGTGSGCLAISLAVNLPAAVIIASDKSVSALHLAQVNA
                     MRHGVAQRILWVAGDLLSHLLSRNLAGKVTAIIANLPYISHAEWEHLSPDVKDFEPRL
                     ALDGGPDGLDVYRRLLDESPGLVSSKGYVLMEVGVGQADQLCREPSLTNAFKVVKVLP
                     DSQGIPRVVCVQRHLV"
     gene            complement(14485..>15354)
                     /gene="prfA"
                     /locus_tag="VLA60_13530"
     CDS             complement(14485..>15354)
                     /gene="prfA"
                     /locus_tag="VLA60_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HSF10428.1"
                     /translation="IAQTRLMTEDLQLDPDMRRLAGEELQSLESQREAMESQVIELLC
                     PADEGDNKNSFIEIRAGTGGSEAALFAGDLLRMYTKFAEAKGLRVELMEFQETGLGGV
                     KEAVLLVEGKGAFGNFKYESGVHRVQRVPTTEANGRIHTSTATVAVMPEVEEVEVHID
                     PKDLRIDTYCSSGAGGQSVNTTYSAIRITHIPTGVVVTCQDERSQLKNRTKAMRTLRT
                     RIGDAEREKQEASIAQQRRSQVGTGERSEKIRTYNFPQNRVTDHRIGLTLHKLDRVME
                     GDLDGLIAGLKAQ"
BASE COUNT         3789 a         4494 c         3436 g         3635 t
ORIGIN      
        1 cttttccaca aattgtgaca ttgacagagg tatctcccct ctatataaga ggacgcattc
       61 acagaaaact taactccctt gcattctctt ctctctaccc ccatgaatgc actgctggtg
      121 tttttccctg gctttccttc cgtaagagtg actggcgtat tttcctgccc gtcgtgcccc
      181 tctccgcgct tcttcctgag caggagtcaa tgcctaaatt tcggcagcca tcagcgtatt
      241 catcgaattt ttttataagt cttttattct ttaagaggcc tatcatggaa agccgtccca
      301 tcatcgaccg cgcaatcgtt gcaaaaaatt ggcatgctcg cgggttttcc tgtggggtgt
      361 ggattgatca tgccggacgt gaatggagag ccgccaccca ggatgtggaa gaaatattta
      421 tggcaatgtc aggagaattg gaattagaaa tcgcaggaga gcgggttcag acatctgcag
      481 ggaaagaagt gcttattccc agcggtgcac cctataccat tcgtaatatt gggggaacaa
      541 ccgctcgttg gctctatggg aaaaaacggt cgccttccgc tccactccaa ccggtcgaat
      601 caaatcgcga tctcatccat accccactgt agagtttgac cgagtgtagc tacttttacg
      661 agtagccgcg ccgtcacttc gcatccttgg cacaacggaa tcctgtcgtc agatcctgaa
      721 aatcaacttc cccttttgag cgggcggtca cccgcaggtt taccggctga tcttcccacg
      781 atcccccacg taatacttta taatcgcctt ccgccggccc ttgaggattc tttttagggc
      841 tctcttcata ataaaaacgt tcataccaat cattgaccca ttcggccgca ttcccggcca
      901 tatggtacaa gccataggcg ctttttccgc cctgttttaa tccgtgacga atactcatgc
      961 ccctggtccc gctggtgacc ggagaaagcg tcaatttata actgacccag ccggtcgtcc
     1021 cctgattata gttggcaata tccacaaatg gctgcatgtg accccatgga tacctccgcc
     1081 catctgtccc ccgagcggct ttctcccatt cggcctccgt gggtaggcgt ttttccaccc
     1141 actgacagta ccgattggca tcgttccagg aaatgcccac ggccggtcga atccctaatt
     1201 ctccagtggc aacaccatca tcccacaacg gtggcggttc aatgctgtaa tcggtatcta
     1261 ataatttctg atattccgcc atggtcactt cgtaccgatc gatagaatag gcatccagcc
     1321 acacggtatg ttccggtcgt tcgttccgag gaccgtcatt atctcccatg agaaattccc
     1381 cttcaggaat cagcaccatt gaacccggcg gtccgtcatc ttgcaccgga gcatccagcc
     1441 aagaatttcc tccttgggat tcttgagcca aggtgggctg gctcaaacca ttgaaaatga
     1501 agactaccgc cagtatgccc aataaccatg tgtgcgtcat aacacctcgc cagaaattaa
     1561 aataattaaa ttttggtccg caacacgcgt cacattccgt cgccaaacac ccacaggaat
     1621 cggaattgaa catcaaacac atttcagtac tttcggggat acctcggcca gcttaatacc
     1681 atacggccgg atgaaatgac gactccaaaa agtatgcttg tttacatctt tatgcggcaa
     1741 ccagaagaaa tccctctctc attcaggaga gtaagattcg acaggcccca aaaaaagtac
     1801 gaccgatatg cttaccatat cgaacttaac tgaacgagtg aagaggcctc agtcgatttc
     1861 cttcaagacc cgctacatac tctgttatca atcactggca atgatttttt taaaaataaa
     1921 aaaaaagccg ttgaagaaac cttcaacggc ttaagctatg gcgtccccaa cgggattcga
     1981 acccgtgttg ccggcttgaa aggccggcgt cctaggccag gctagacgat ggggacgcag
     2041 taattcagga tgtgtattag gcctttgtat gataaacacg gaagcgatgg acccggcatt
     2101 ctatcaatgg ggaaaacacg agtcaaacca ggaagttatt ctcaaccatc tgaatgctat
     2161 cagctcacac aacagtatga gcctccgact ggaactttgc ctcccatttg tgggatctcc
     2221 cggagaacgc ctcgacaccc gtgaggagaa accacaataa ttttggccaa acccataatc
     2281 aagaaagatg gtgaaggagt gggcctgtaa gacggattct gtactagatg aaacgagttt
     2341 catccagtgg tgaccatttc tctggaacct aggttaccct aggcttcaag caacctaccc
     2401 gagaacctcg accgggccgg tctctctccc agtccgagaa gaaatcgctt tcttcgacaa
     2461 accgggaatc gtccccctat ttggttttgc tccaggtgac gcttaccctg ccacctgtgt
     2521 caccacaggc gcggtgggct cttaccccac catttcaccc ttacctggca aaaaccgagg
     2581 aatttgccat tggcggtata ttttctgtgg cgctggtgtc ggattgctcc gcctgggcgt
     2641 tacccagcac cttgcccttt ggagtccgga ctttcctctc acgaaaaatt ccgtgagcgg
     2701 ccacccggcc cactcgcacc atcagtatct atcgtaggtt gagaaaatga tgaaagcaag
     2761 cggcgcgtca catggaggaa aagctattcg gtcgtttcct cttcaagatg ccgagccata
     2821 tagagaatac ggtggcaata agaacagtcc aatagttcgt caccgcgttt cacctccgca
     2881 accagttgcg gtggaagctg aagctgacac cctccacagg ccccgtcacg aagctgggcg
     2941 acggcatatc ctttcctcaa ggtctttaat cggttgtatc gaaccaaaag tggcttatcc
     3001 acctgcaggg aaagagtggt ttgctgacgg tccaaatcgg ctaattcatt cgccaaattg
     3061 gcaaaatgcg tctccagtct cgccttttcg ctatcaaaaa ctttctgggc ttctatggat
     3121 tgctcttcca gctctttaag ggcctgctcg ttctgttcca cccgctccat gatttccagc
     3181 acattctcct caatagagtc ttttttcttc cgagctaact ctatttcaaa cagatgcgcc
     3241 tggtattctt tgttcgtttt taactcagaa agccgatttc tgattttctg gagttgttct
     3301 tcttgagtag cgagttcccg ttcagagctt cgtcgttgtt tgacgagcga ttcacccgta
     3361 tttttaagga cttgtagttt ggcaagaata tcttgaagcg gagtttcagc ggatttgaga
     3421 agctctggag ctttccgcaa ctggtcttga atctgaaaaa ttcgaagatc aaatttttga
     3481 agattaatca ggtattgcaa ctgcaaattc acaaagtcct caatatccta gaacatgttt
     3541 gctcaccggt tactcaagtc cggccctgtc tcgtggtggg cccaccagga cttgaacctg
     3601 ggaccaactg attatgagtc agccgctcta accaactgag ctatgggccc gagatcataa
     3661 cggcgcacac acccttttac cataaatggt gctcaggaaa aactcccccc tgcgaatttt
     3721 attccctcgc caggggtaaa ctcaggtaga agcggttaat aaggcctttt tctgacgaag
     3781 cacattggac tctgcgacca aagcatcgac caagtcctgg cgcccctccc gttgtgcaac
     3841 cttaagttga ttaatcacct cgtccatgag gcgttggatc tggtttttct ttaaaacacg
     3901 gagacatccg gcgacatgaa tagacacttc gtcacaattg tattccccca cgctcaaccg
     3961 ggaaatgacc ggttcataag ccggatcctg actgaattcc gcatgcaagg catcgaggtt
     4021 caagttccct tcgtcatccg aatgctgaaa ggccttgcca agaatatggc gatacagtgg
     4081 gacagtaaac gcaccctcat caagttgccg aatctgatcc ggttccagac ggccttggag
     4141 taataataca ataagatccc gttcctccgg cttgccttga ggaatggcca gattcgtggc
     4201 agcttgctcc ttccccttag ccgacggggc gacactcatc ggccgggaaa tagtcgggag
     4261 acgtttacga agtaattccg gtcgaatacc caaccgttct gagaccactt tcagatattc
     4321 ttccttttct atgggatttt tagtcttatg aagaatcgca aggatctcat ccgcccgctt
     4381 gacccgatct tgaatagaat catttttggc tttatccaac acagacatga tagcaaaatc
     4441 cagcaacgtc gcggctttcc cctccaactc acgaaacgcg gacaccccgt ttttccgaat
     4501 aaatgtgtca ggatcttcac cagccggaag cagcagcact cggacttcaa tgccggaatt
     4561 cataaacaaa tcaagcgtcc gcaaggccgc accgatccct gcggcatctc catcaaagac
     4621 gagcatcacc gacggggcca atcgccgtag aatctggaca tgatcggagg ttaacgccgt
     4681 tcccaagggt gccacggcat tttcaatccc gttttggtga agagcgatga catcaaaata
     4741 cccttctacc aaataaaacc tccccgaagc ggtggctgac tgcctggcct tgtccattcc
     4801 atagagggat ctccccttaa agaataagtc ggtttcgggt gaattgagat atttgggaga
     4861 ggccccatct ttcaggattc gacccccaaa tcccaccacc tgcccacggg gatcagtaat
     4921 gggaaacatc acccgatcac gaaagcgatc atacgtggat attctggtgc catcctcaga
     4981 ctctttccgc accaccaatc cagcctgtat gagttcttca ggcttgacag actgctgttc
     5041 cagccatttg gaaaggccat tccatccttc cggaacgaat cccaactgaa atgtgttcca
     5101 ggattccctg gtaatccccc gctgatccaa ataatccctg gcgacttgac cttcaggatt
     5161 gttctgaaga ttacggtgat accaggatgc ggccaattga tacagatgaa aataccgttc
     5221 acgagataag cctgatgtgg tcttccccgc tgattcccga cgttcaggca ggggcacccc
     5281 tgtccgttgg cttaactcac gcagagcctc catgaaatcc atgccttctt gtttcatcaa
     5341 aaaggtaaag gcatctcctc cgacactgca gccaaaacaa taaaacattt gtcgaacggg
     5401 attgaccgaa aatgagggag ttttttctga atggaagggg cacagccctt taaagttttg
     5461 ccccgctttg gaaagactca cgtatgtgga aatgagatca ataatatcaa tgcgatctcg
     5521 aatttgttga agaatctctg gaggaactcc tcgcttttcg cctgtcatct cacttctcac
     5581 cacggactgc gttagggaca cagcatgaat gtcgttccct tcggatacca tatactctcg
     5641 gggcactttg tccccggcaa cccaacatta acccggaggc cacgcaaatg tccgcccacc
     5701 cataacgtga aaatgcaaat gaaaaacact ctgtccggct cccggtccga tattcgcaac
     5761 gacccgatat ccgccaccga caatatcccg ctgcctggcc atcttggcac agacgaccat
     5821 caggtgacct aacaaggcgg catccgtctc tttcgtatca tccaaagaca ctacgtgccg
     5881 cttgggaatc accaacatat gcacctgcgc ctgaggattg atatcgttaa acacgaccgt
     5941 gtgttcatcc tcatacagtg tgtcggcggg aatagccccc ttgacgattt gacaaaaaag
     6001 acaatcaccc atgtggagag cctccttccg gctgtcgaat tcctgatttg ccgaatcgct
     6061 tgccaagctc ttgatagacg tcttcaggag gaatggaaaa atgcccgagc atgatgaggg
     6121 tatgaaacca caaatcagcc atttcataaa taatttcttc ccgatcacca tttttcccag
     6181 cgagaaggac ttcgccggct tcttccgcca cctttttcaa aatacgatcg aggccttcct
     6241 ccattaattt cgacacatag gaatcagggc tcgggttggc cttccgatcg ccaaccattt
     6301 catacaggcg ccgggtaatc cctccccagg ccgtaccgga gaagaaagcg gctggctcct
     6361 cagaagtcga agaaggtagg ggagaaaaaa aacatgaacg ggctccggta tgacacgtcg
     6421 gtccaatggg ctgggctttc accaaaatcg tatcccgatc acaatccaca aacatttcct
     6481 ttaacagcaa ttcatggccg gacgtctcgc cttttttcca taaccgtttt cgcgatcgac
     6541 tccagaaatg aacccgttgg gaatcgcaag tcatctccca cgcttgttgg ttcatgaacc
     6601 ccaccatcaa gacggtccca tccaaccaat cctgcacaac acaggggagc aatccttgct
     6661 catcaaactg caatgtgctt gtcgaagaga cttcgctcat gggtcgttat gaaattgaaa
     6721 ccggcacggt tcccggacgc acagggatac cctgttgagc caaatacgat ttggcttgcc
     6781 cgatggaaaa cgtttgataa tggaaaatag atgccgccag caccgcatct gctttcccca
     6841 catggaacgc ctcatacagg tgctgaagcg tgccagctcc tccggaggca atgacgggaa
     6901 tagagacgga ctcggaaata gccgccgtta aggccaaatc atagccgtcc tgctttccgt
     6961 cctgatccat actggtcaaa agaatctctc cggcgccata gtccttcatc cgtttccccc
     7021 actcaacggc gtccagaccg gtcgagttcc gccctccatg ggtaaacact tcccacccgg
     7081 gctcaccccc ttcacatgtg gtcgcccgct tcgcatcaat cgccacgacg atacattgcg
     7141 agccgaaacg attggcggca gcttttacga aatcaggatg ctccaccgcg gccgtattaa
     7201 tactgacttt gtccgctccg gcttctaata accggcgaat atcctcaatg gtccggatcc
     7261 ctcccccaac cgtgagcggc ataaacacct gttccgccgt ccgaatcacc acatctaaga
     7321 tcgtatcccg cttttcatgt gaagcggtaa tatccagaaa acacagctcg tccgcacctt
     7381 ctcgatcata cacctgcgca atgtgcaccg gatctcccgc atcgcgcaaa ttcacaaacg
     7441 agaccccttt caccactcgc ccgtctttca catccaggca gggtatgatt ctcttcgtca
     7501 gcatgatgct taagacgctt ctgccaaggc caccatttca agggccgcct tcaagtccaa
     7561 ggtgccttca tatagggcct ttccgataat gattccggaa attctgcttc ccagggcctg
     7621 aatagtctgt acatctttca ggacggtgac tcccccagaa gcaataacgg gaaacggcga
     7681 gatgtccacc gcttctcgca gagctgaaat atttggtcct gccaacatgc cgtcccggga
     7741 tatgtccgta aagaccatgc ctcccaaggg caagtctttt actgattcaa agacctgtcg
     7801 tggggtcaga ctggacacgg tcttccaccc atgcactgcg actaaaccct gtttgacatc
     7861 aatcccgatt aaaattttat caggaaactc tgtcgcggct gctgccaaaa aatccgcatc
     7921 ctgtaaggcc gctgttccaa tcaccacccg gtccactcca caggacaagt aggcccgaat
     7981 ggttgacgtg tttcgtatgc ccccaccgac ctgcacggga atggctaacc gtttggtcat
     8041 ggcttcaatg tgtgatagat gctgaggtgt tccggtcacc gccccgtcta aatccaccac
     8101 atgaaggtag cgggcaccaa gactctgcca atgttccgcc atcgccgcag ggttatcgga
     8161 atacctggtg acctgatcca tctcgccctg tcgcaggcgg acacatttcc cgtccttcag
     8221 atcaattgca ggaatcagta gcatcggtta gggattctcc ttcaaggatg gagcaggaag
     8281 aggaggcccc tgttcgccca agccgacggg gaaggccttc atcactttgt tgacacccaa
     8341 ttcagcaagt ttatcggcag tcacaaaccc tccacccttt tcaaaaaaac ctacaacatc
     8401 ctggttaagc ggtttttgtt cctcaaaaaa ttccccggtc cacaagacca ttccatcaga
     8461 cggtctgagg agatacactt caaaccctac tgcggccggc ttggctccaa gcttgctccc
     8521 ttcacgctca cggtatgtcc ggacgagtcc ccccacaact ccatcaactt ttaagcgggt
     8581 tccgacctct tgggccatga ccggcaatga cgattctcct tccgggcttt ccttgcccat
     8641 cacggcagaa gactcctctg gaccaatcac ccgtaaggcc ggccggccgg ccaacacaga
     8701 agccacctgt gtcgtaatgc gatgtgccgc cgtttcaggc accacataac gaacttttct
     8761 ggattctgtc ccatccgcct gatcagtccc cggcaaccga aattgtgttc gaatttcttc
     8821 cggatcccta atgcctccac gtgtcgagct actcgccaac tgaggggttt tgatggtctg
     8881 aaaaggaagg atggcgagac tggatatacg ggagggatca aattgtggag cggcctgaat
     8941 ttgaacttta gggggaacac agcctacacc gagacaccac catccgataa ggctcatgac
     9001 caggaaccgg gaaggcatcg gtctcgaccg cccattgatc gcaaaacaca ttcccctcat
     9061 tgccaatcac caaaattctt caagacctgc aacccgacgg cctgactttt ctcgggatgc
     9121 cattgggtag caaacacatt gtctttccag acactactcg caaaagaaat tccatagtcg
     9181 gtttgggtac tgacgagagc acaatccgtc ggttccacat agtaggaatg cacgaaatac
     9241 acataggagt cggaggcaat gccacgtaat ggcggagcaa ccgtgttgac ctgaatcgta
     9301 ttccaaccca tatgaggaat tttaaacgac gtcgctccat catcggaggg acggggaatc
     9361 gctttcacct tcccggccag gatccctaac ccctggtgac agccaaattc ttcgctttcc
     9421 gaaaacagca attgataccc taaacaaatg ccgagaaaag gttttcccga agcgatggaa
     9481 cggtatatga catccatcaa ctcaaagcga gccaggttgt ccatgcaatc cccaaacgcg
     9541 cccacacccg gcaatacgac atgactggcc tggtcaatca cgagaaggtc tcgagtcacg
     9601 accgcgtcat ggcccacgca ttcaaaccct ttctgcacgc tccgcagatt tcccatccca
     9661 taatcaataa tggcaatcat gtcccggctg ttatcctctc tctccgcccc ctgattctca
     9721 cccttcagag caaccctttc gttgagagca ctcccttaat tcgttcttcc cgcctaaccg
     9781 cttgatccat ggctttcgca aacgccttaa aaatcgcttc catgatgtga tgaggatttc
     9841 ttccatacag cacattcaca tgaaggttta accctccctg actcacgaat gcctgaaaaa
     9901 aatcttcaaa caatcccaaa tcaaacgact taatataccg cgtgggtaac accacattgt
     9961 atacgagata cggccggcca ctcagatcca cggtcacctg ggccaacgtt tcatccaacg
    10021 gcaccgtcgc ccatccgaat cgggtgaggc cttctttggt ccccagagcc tgggttaaga
    10081 cctgccccat gacaatcccc acgtcttcga tcgtgtgatg gtcatcaatc tcgatatccc
    10141 ctttcgcttc caccgtcaga tcaaaaaatc catgtttacc gaataaattc aacatgtgat
    10201 ctaaaaacgg gatcgaggtc ttaatctgat tgttgccgca tccgtcgagg ccccattcca
    10261 ctcgaatggc cgtttccgcg gttttccgtt cttgagaagc cgttctggga acaagatgtc
    10321 ggttcatgtc catcgactct ccattgatcg cccatgtgca tccaggcctt ccagcagaga
    10381 cagccgcatc actggagcat gggctttgcg caacgcggcc ttcgaatagg ccacaacatt
    10441 gctggttttc atgtactcat ccacacccaa acacgaaaaa aatctggcac tcccaccagt
    10501 tggtaacaca tgattgggcc ctgccacata atccaccacc gcgggagggg tatagcgccc
    10561 aacaaaaatg gcacccgcat gacgtatacg tgacacatag tctcccgccc gatccacatt
    10621 taatgataaa tgctccggcg caatccgatt ggctaattca aacgcctcat caaaggttcg
    10681 cacaacaaac gccagggcat gattgtgaat tgatcgggac gcaatcgcct ggcgtcccaa
    10741 ctgtgcaagt tggcgggtca cttcagtcac aacccgtctg gcaagagtaa gagaagtcgt
    10801 caccagataa acacaggctt cctcatcatg ctcggcttca caaagaagat cagccgccac
    10861 atgcagtggg ttactggcgg agtcggcaat gaccagcaac tcactcggac ccgcaatcat
    10921 atcaatgtcg acctggccgt agaccaggcg tttggcggtg gccacataca tattccctgg
    10981 accgacaatt ttatccacgg gccggattgt cgaggtgcca aaagccaagg ccccaatggc
    11041 ttgggctcca ccgacccggt agatttcatt cactccggcg acatccgccg ccgccaacaa
    11101 cgcagggtgg atgatgccat tcggtgtcgg cgtacacatg acgactcgct tcaccccagc
    11161 cactttcgcg gggatggcat tcatgagcac cgatgacgga tacagggctt ttccgcctgg
    11221 aacatatacc ccgacggcat ctaacggcgt gatccgttgg cctaattgaa tcccctgttg
    11281 acgatagacc caggatttcc ttcgttgtcg acgatgaaag gtccgaatac ggttcgccgc
    11341 atatttcagc gccttaatat ccgaggacgg tagttcggcg taggcacgct cgacgtcttt
    11401 cgacgtcaca cgaaggccgt caggactgat tcggacatga tcgaatttcc aggtcaatcg
    11461 ctggagtgcc tgatcgccct cctgccgaac agcatccaag attgtcctga cggctttttc
    11521 aaccttgtcg ttttgttgag ctgcccgctg acagacacga ttcaccgctt gcgaaaactc
    11581 ttggtccttg tgggaaacaa ttttcatgca tgaacctttt tggagcggga acgagaaaga
    11641 attttcttta cccgggcaat cagttccgta atcacggcat gtttcatttt taagcttgcc
    11701 cgattcacaa tcaatcgggc cgatgaccag gcgatcactt catcctcgac cagattattg
    11761 gctttcaggg tgtttcccgt ctccaccaga tcgacaatcc gatcggccaa tccgaccaat
    11821 ggagccaact cgatcgaccc gtagagctta atcaattcca caggcaaccc ttgctggtta
    11881 aaaaaacgtt cggtgatcat gggatatttg gttgccaccc gaagcttgga agacatccgt
    11941 tgcacaagct gtggcccgtt cggcctggcg actaccagcc gacagactcc caagttcaag
    12001 tcaaccggtt catagacatc cgggctctgt tccaacagga catctttccc aaccacccct
    12061 aaatcggctg cgccatattc aacataggcc ggcacatcac ttgggcgtac aattaagacc
    12121 cggtgcccat gctccgggaa ttccaaaatt aatcgacggt cgtccgctcg gacatgatat
    12181 cccgtatagc ctgcctggcg gaacaattca agagtcggat caagaagttt acccttggaa
    12241 agggcaatgg taatcccttc gctcatgtca cctcgcgaac gcgaacaatc ctcgcaccca
    12301 gggcctgaaa tttttcttcc agccgctcat atccgcggtc taaatgataa actcgggaca
    12361 caatcgtttc gccaacagcc gctaacccag ccaggaccaa acctgcactg gcgcgtaaat
    12421 ccgaagccat gacgggtgca ccggtgagca acggcacacc tttgacgacg gcatgcgggc
    12481 catcaatctc aatcgacgct cccatccggt gaagttccgg gacgtgcaaa aatcgtcctt
    12541 caaaaatggt ttcggaaatc acgctcacgc cttctgccac cgacatcaac gccatcattt
    12601 gggcttggag atccgtcggg aagccgggat aggggagcgt cttaatttcc accgctttga
    12661 gcggacccga ccggcggatt cgcacaccgt catggctttc ggttaactca gcacccattt
    12721 cctgaacttt tttcaggacg ctgcctaaat gatctgttac gcaccccttt accatcacat
    12781 ccccaccggt aatggctccg gccatcaaat aggtaccggc ttcaactcga tcggggatca
    12841 cccaatgccc tgccccatga agttccttca ccccttcaat tgtaatggtg tccgttccca
    12901 tcccatgaat tttggcaccc cgcttggtta agaaggcaca caggtccgtg atttccggtt
    12961 cccgggcgac attatgcaaa ctcgtggtcc cttgcgccag gcaagccgcc atgatcaggt
    13021 tttccgtgcc cgtgaccgtc gggaaatcca agtcaatcct tgctccttta agccgaccgg
    13081 ctttcgcatg gatatagcca tgctccactt gaatttcagc ccccatcatc tccaatcctt
    13141 tgaggtgaag attgaccggt ctggtgccga tcgcacaccc acccggcaga gaaaccttgg
    13201 cttctcccag tcgagcgagt aatgggccta aggctaaaat agacgctcgc atcgttttga
    13261 ctaaatcata cggcgcttca atcgaatgca cctgagccgc atccagcctg accctggcgc
    13321 cttcctcttg aaccgccact cccaacaagg ccagtaattt ccccatggtc atcacatcac
    13381 gaacgtgtgg aacatggtca atgacgcatt ccccgccccc taataacgtc gctgccaaaa
    13441 tcggcaaggc ggcattcttg gctccgccga tttccacgga cccctttaat ggcaatccac
    13501 ctgtgattcg aatttgatcc actgacagta ttcctggtct acacaagatg ccgttgcacg
    13561 cataccactc gtggaatgcc ttgggaatcc ggcaggacct ttaccacctt aaacgcattc
    13621 gtcagggaag gctcccggca cagttggtca gcctgaccaa ctcccacttc catcaggaca
    13681 tatccctttg aggaaacaag tcccggcgat tcatccaata accgccggta cacatcgagt
    13741 ccatccggcc ctccatccaa cgccagccgt ggttcaaagt ctttgacatc tggagaaagg
    13801 tgttcccatt ccgcatggga gatatacggc aggttagcaa taatggcggt cactttacct
    13861 gctaaatttc tggataacaa gtgggaaagc aggtcaccag ccacccacag aattcgttgc
    13921 gcaactccat ggcgcatggc attgacctgt gcaagatgta gcgcggagac tgacttatct
    13981 gatgcgataa tcaccgctgc aggaaggttg accgcgagac tgatggccag acatccagaa
    14041 cccgtcccga catccagaat caccggacag gcgtcagccc ctagaagtcc aatcgcctct
    14101 tccaccagca attcggtctc aggtcttgga atgagcaccc caggtcggac gagaaaatcc
    14161 aaaccaaaaa aatcttgatt ccctaataca tattgcagtg gttcatgtaa ggcacggcgc
    14221 tgaaacaagg ccacagcctg ccgacagact tccgcatcaa cgggagtgtc tggattcgca
    14281 tgcagttgca acctcgtaat accaagggca tcctcaagaa tccacagcgc ttcatggcgg
    14341 gcgttggcaa tccccgcgtg aatcagcgtc tcaaccgcac cacgatataa ctgtgaatag
    14401 gaagtgaagg ggggcatctg agcctcatag agcacacgcg ccttgaaccg ttgaaagaga
    14461 aggaccggcg ttacacgaag ggcatcattg ggctttgagg cccgcgatta acccgtcaag
    14521 gtcgccttcc atcactcgat ccaatttgtg caacgtcagc ccgattcgat ggtcggtcac
    14581 gcgattttgt ggaaaattat acgtgcggat tttctcactc cgttcccctg tcccgacttg
    14641 ggaacggcgt tgttgcgcaa tagaggcttc ctgtttttct cgctcggcat caccaattcg
    14701 tgttcgaagg gttcgcatgg ccttcgttcg attttttagt tgggatcgct cgtcctgaca
    14761 tgtcacgacc actcctgtcg gaatatgcgt aatccggatc gcagaatagg tcgtattcac
    14821 actctgtcca ccagcccctg atgaacaata ggtgtcaatc cgtaaatctt tcggatcaat
    14881 gtgcacctcc acctcttcaa cctccggcat caccgcgacc gtcgccgtgg atgtatgaat
    14941 tcgtccatta gcctctgtcg tcggaacccg ctgcacccgg tgcacaccac tttcatactt
    15001 aaaatttcca aaggctccct tgccttcaac cagcaaaacc gcctctttga ccccaccaag
    15061 accggtttcc tgaaattcca ttaattcgac ccgtaatccc ttagcctcgg caaacttcgt
    15121 gtacatccga aggagatcgc cggcaaagag tgcggcctca cttccacccg tccctgcgcg
    15181 aatttcaatg aacgaatttt tattatcccc ttcatcagcc ggacagagca attcaatgac
    15241 ctgggactcc atggcctcac gttgactttc caggctttgc aattcttcac cggccaaccg
    15301 gcgcatgtcg gggtctaatt gcagatcttc cgtcatcagg cgagtctggg caat
//
LOCUS       DATIAL010000436         3815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_831864, whole genome shotgun sequence.
ACCESSION   DATIAL010000436 DATIAL010000000
VERSION     DATIAL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831864"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="VLA60_13535"
     CDS             <1..2043
                     /locus_tag="VLA60_13535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF10429.1"
                     /translation="QEVLGVMGLFARHPLTEFTLKSLGMVADRITVALERQVARDEKN
                     KLTLFKQRLLASAGEGIYGLDLDGNTTFVNPAVLQMTGYEEDELLGHSQHAILHHTKP
                     DGSPYPPEECLIYAALKEGSVYHIDTEVFWRKDGSFFPVEYVSTPIRNDLGQLEGAVV
                     TFQDITHRKRAEKVLQESEERFRFLNEAIPQQVWTARPDGSLDYVNRRVLEYFGSSFE
                     EMIGHGWQRFLHPDDLPGCLDCWDRARETNQPYEIEFRLLRGSDHAFRWHLGRALPVF
                     NSEGQVVKWFGSNTDITQLKQLENQVRQSQKMEAIGTLAGGIAHDFNNILMAIIGYTE
                     LAKQSARENVRAQKNLDEVLVAGQRAKELVQQILTFSRQTEQMQQPLDLQLLVKEIGK
                     FLRATFPATIDIRQQFTEATSIILGDPIQMHQVLMNLCANAEYAMRGSGGLLELKVER
                     VIGEPDGIGAHPDLKGGAYVCLTVRDTGTGMTPDVLQRIFDPFFTTKRVGEGTGMGLA
                     VVHGIVISYGGVIKVESEPGQGTMFSIYFPEMEPANYPGEKTPLPQEVFMGRGHILFV
                     EDEEPLARLGEEAMKGLGYEVMVRTSSIEALETFRADPFRFDAVVTDQTMPHITGEAL
                     ARQLLELRPDVPIILCTGFSHSMTSEKAKAMGIRAFLLKPLLIKDLAWTLREVLHH"
     gene            complement(2232..>3815)
                     /locus_tag="VLA60_13540"
     CDS             complement(2232..>3815)
                     /locus_tag="VLA60_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HSF10430.1"
                     /translation="KPGSVGPPLTSVEVRIDQPNEEGIGEVVVRGPNVMKGYDQAPAE
                     TAEAIRDGWFFTGDLGYLDSDGYLFLTGRIKELIVTPGGKNILPEELEEAYQQNPAIA
                     ELCILGLPRPGEEGEHLHAVVVPNFDYLREHKIHDSATYIKDALNSVAATLPSYKRIS
                     GVTFIKDSLPRTRLGKIQRHLVRAMTQSKQTAVEPAPEQEPETDQEIRQTTIGQAVIQ
                     TLVGLTSADHALRLDDHLDLDLGFDSLKRVEFQAALESRLGPVPETFMGEVVTVRDVI
                     TKLLALQQIPVEDTETTSSWHQIFENPLPPALTKTVLAPLSRGNKIIGQIGMAMVGSL
                     SRIAFPLTVKGIAHLPKEGPFILAANHLSFIDPFIILATVPPSTFSQLYTLGWEPFFR
                     SPFRRWVARVGHVIPVGPETPLVNVLKASAALLRNGKSLLIFPEGERSLDGQLLPFKK
                     GLGVLACELHIPIIPVKIEGSFQVWPPDAKTPQRHPISLTFGEGQTFTPSMVETWTTR
                     GEDPYVVATQFIRDAVASL"
BASE COUNT          912 a          886 c         1065 g          952 t
ORIGIN      
        1 caggaggtgc tgggagtgat ggggttattt gctcggcatc ctttgacaga gttcacgctc
       61 aaaagtttgg gaatggtggc cgaccgtatt accgtggccc ttgaacgtca agtggcaaga
      121 gatgaaaaga ataaacttac cctcttcaaa cagcgccttc tggcatcagc gggggaagga
      181 atttacggcc tagatcttga cggaaataca acttttgtca atccagcggt cctgcaaatg
      241 accgggtatg aagaagacga actgcttggt cattcccagc acgcgattct ccatcacaca
      301 aaacccgatg gttcacccta tcccccggaa gagtgcttaa tttatgccgc attaaaggag
      361 ggctccgttt atcatataga cacggaagtg ttttggcgta aggatgggtc gtttttccca
      421 gtcgaatatg taagtacgcc tatccgaaat gatctgggtc aactggaagg ggccgtcgta
      481 acttttcaag atatcaccca ccgcaaacga gccgaaaaag ttcttcaaga gagcgaagag
      541 cgatttagat ttctgaatga ggcgattcca cagcaggttt ggactgcccg gcccgatggt
      601 tccctcgatt atgtcaatcg acgggttctc gagtatttcg gctcatcctt tgaggagatg
      661 atcggtcatg ggtggcagcg ctttctgcat ccggatgatc tgcctggatg tctggattgc
      721 tgggacaggg cacgtgaaac caaccaaccc tacgaaatcg aatttcgcct actgcggggg
      781 tcagaccatg cctttcgatg gcatcttggg cgggctctac ctgtttttaa ttcagagggg
      841 caggtggtga aatggtttgg atccaacaca gatatcactc aattgaagca gttggaaaat
      901 caggttcgcc aatctcaaaa aatggaggcc attgggaccc tggcaggtgg tattgcccat
      961 gatttcaata acattcttat ggcgatcatt ggctatacgg aacttgccaa acagagtgcc
     1021 agggaaaatg tcagagccca aaagaatctt gatgaggtgt tggttgccgg gcagcgcgcc
     1081 aaggaattgg ttcagcaaat tttgacattt agtcggcaaa ccgaacaaat gcagcagccg
     1141 cttgaccttc aactgttggt caaggaaatt ggcaaattcc ttcgtgccac gttcccggcg
     1201 actatcgaca ttcgtcaaca gttcactgag gcgacctcga tcatattagg agatcctatc
     1261 caaatgcatc aggtgttgat gaacctgtgt gccaacgccg aatacgccat gcggggaagc
     1321 ggaggtcttc ttgaattgaa agtggagcgt gtgattggcg aacctgatgg gatcggcgca
     1381 catcctgatc taaagggagg tgcctatgta tgtctgacgg ttcgagatac cggaacgggt
     1441 atgactccgg atgtcctcca acgtatcttt gatcccttct ttaccacgaa gagggtcggt
     1501 gaagggacgg gaatgggctt ggccgtggtc catggaatcg tcatcagtta tggcggtgtt
     1561 attaaggtgg agagtgaacc aggacaggga acaatgttta gcatttattt tccggagatg
     1621 gagccggcga attatccggg ggaaaagact ccattgccac aagaagtgtt catgggccga
     1681 gggcatattc tttttgtgga agatgaggag ccccttgcca ggcttgggga ggaggcaatg
     1741 aaggggttgg ggtatgaggt catggtgcgc acgagtagca ttgaagctct ggaaaccttt
     1801 cgggccgatc cctttcgatt tgatgcggtt gtgaccgatc aaacgatgcc gcatatcacc
     1861 ggcgaagctc tggcgcgtca attgttggaa cttagacccg acgtgcctat tatcctgtgt
     1921 acgggtttta gtcacagtat gacctcggaa aaggccaaag ccatggggat tcgggctttt
     1981 cttttaaagc cgctgttgat taaggatctc gcgtggactc ttcgggaggt tcttcatcac
     2041 tgacttatta aaaaagaccc ttcagttccc tccgaccaaa tgaggtattt tttggctcta
     2101 gttttgtaag aaggatggta cctgcaactt tcccaatttc cttagaagga caattttctg
     2161 ctttcctcca gccattgaat tcactctaaa cacttacaag aggaaccttc ttgaaaatgg
     2221 tcacccagga attagagtga tgccacagcg tcacgaatga attgagtcgc gaccacatag
     2281 gggtcttccc ctctggttgt ccatgtttca accatggatg gggtgaaggt ttgcccttcc
     2341 ccaaaggtga gggagatggg atgacgttga ggtgtcttgg catcgggagg ccagacctga
     2401 aaacttcctt cgattttgac cgggataatg gggatgtgta attcacaggc aagaaccccg
     2461 agccccttct tgaatggaag gagctgtcca tcaaggctcc gttctccttc ggggaaaatc
     2521 aggaggcttt tcccgttccg gagaagggcg gctgaggcct tgaggacatt gaccaggggc
     2581 gtctccgggc ccacgggaat cacatgaccc acgcgtgcga cccatcgacg aaaaggtgat
     2641 cgaaaaaacg gttcccagcc aagagtgtaa agttgtgaaa aggtggatgg tggcacggtc
     2701 gccaaaataa taaagggatc aataaaactc aggtggttgg ccgccaggat aaagggtcct
     2761 tccttgggca aatgtgcgat gcctttgaca gtcaatggga aggctatacg ggaaaggctt
     2821 ccgaccatag ccatgcctat ctgcccgatg attttattcc ctctggaaag cggcgcgagg
     2881 acggtttttg tcagggctgg agggagtggg ttttcgaaga tctgatgcca ggaggaggtg
     2941 gtttctgtgt cttcaacggg aatttgctgg agggccaaga gcttggtaat gacatcacgc
     3001 accgtaacga cttctcccat gaacgtttcc gggaccggtc ccaaccggct ttcgagagct
     3061 gcttgaaatt cgacacgttt caacgagtcg aatcccaaat ccagatccag gtgatcgtcc
     3121 aatcgtaggg cgtggtcggc tgaagtcagc ccgacaagcg tttggataac cgcttggccg
     3181 atggttgttt ggcgaatctc ctggtccgtc tcaggttctt gctccggtgc gggttcaacg
     3241 gcagtctgct tagattgcgt catggcacga acaagatgtc gctggatttt ccctaagcgg
     3301 gtgcgtggga gggaatcttt gatgaaggtc acaccactga tccgtttgta ggagggcagg
     3361 gttgcggcga cactattcag cgcatctttg atataggtgg cggaatcatg aattttgtgt
     3421 tctcgtaaat aatcaaaatt tgggacaacc acggcatgaa gatgttcgcc ctcctcacca
     3481 gggcggggga gtccaagtat gcagagttcg gcgatcgccg gattctgttg gtatgcctct
     3541 tccaattcct cgggaagaat gttttttcct ccaggtgtga cgatcagttc cttgatccgt
     3601 ccggtgagaa acaggtatcc gtctgagtcc agatagccca gatctcccgt aaaaaaccag
     3661 ccatcacgaa tagcctctgc tgtttcggcc ggggcctggt catagccctt catcacatta
     3721 ggccctcgta ccaccacttc tcctattccc tcttcgttcg gctgatcaat tcgcacctcg
     3781 acgctggtga gtggtggacc gacggaaccc ggttt
//
LOCUS       DATIAL010000437         3462 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_403486, whole genome shotgun sequence.
ACCESSION   DATIAL010000437 DATIAL010000000
VERSION     DATIAL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403486"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VLA60_13545"
     CDS             complement(<1..479)
                     /locus_tag="VLA60_13545"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HSF10431.1"
                     /translation="MVCTALLVSLGHSATYDCFDESGTRVLTDNPAQLFNCTLLPTQS
                     PSLSNQASSTTATLQAIPKSIPDPAPPDAQISTQVSSLNEEEQERPVNQEITIPLTKS
                     GGSFVVPVMLNQERPAQLIVDTGASMTVLSTQVAIDLGILGTTDNELLTVNTAGGSV"
     gene            complement(600..1202)
                     /locus_tag="VLA60_13550"
     CDS             complement(600..1202)
                     /locus_tag="VLA60_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10432.1"
                     /translation="MTNQRKWLVLGGLLLAWGMVTIFQMWDSAPTDTLPSSATSGVPL
                     PPIKDLELDPAFSNPRQTAKFSLPRNIFSPLGMSQVAHQKKIDSAPTSRLPKTAPSPA
                     PPQPPPPPPGPSMADLAGQRAREQLNQFRFLGYLTKGGESQAFLTNGQAIYIVKQGEM
                     LEGRVQVNKIEPETVVLSTQVLETGSHVQATIPLTPDTSG"
     gene            complement(1202..1774)
                     /locus_tag="VLA60_13555"
     CDS             complement(1202..1774)
                     /locus_tag="VLA60_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10433.1"
                     /translation="MNSFQIIQGSKTTIRISKYLGPLFIVTVLAGLSSVGMFAFLFYP
                     AQDRFQHITALNQASLHTQATMQTARQTQTILSTMWSALPDSGEFTDLSLDIADLAKQ
                     NHVQIPGMGYDFKPLAHKLATKGTFAFEAAGPYEAIRKFIFELEQRWPYLFIEKLSVE
                     TSKKQKGVIFKLKVSTFLKEFPEPVKKTKA"
     gene            complement(1771..2385)
                     /locus_tag="VLA60_13560"
     CDS             complement(1771..2385)
                     /locus_tag="VLA60_13560"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HSF10434.1"
                     /translation="MMIPRVHRNLSAPGIPLLQLSQLGLTLLTAGALALTASFWWEGN
                     SLHEQIDVLEEQAAGVETANQQLVTQARAAGLDLSHQAIRSIPSKVTFVKQVRERVGF
                     SWTQLLTDLETAIPSDITLNSVSLDEKTDTILLQGSAASLPDLNRLIHQLEKHAAFQN
                     VLLSQHSTKTTKEEQGGSHSVFSLTVTYDARHPTSHLTGVPRQR"
     gene            complement(2382..>3462)
                     /locus_tag="VLA60_13565"
     CDS             complement(2382..>3462)
                     /locus_tag="VLA60_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10435.1"
                     /translation="HIARLPLSPGGLTLSSTKLNIANTDEFLTQLKALAKPYRRPISI
                     ALSLPDICARTSIFDFASFPTKKPEQTALVNWRFQQDLKLDTSQSRLAFGLYVPTSVR
                     DSPSPDTTEHVRVLGTAIRHEIIEQYENLCLEADLIPTSVGMSGLDVFDLYRPNIQDR
                     LTAENSSWTSHPPTFMFLFISHWGFTFLAFQDGSPRFIRTKALAIKTEAHDLQVPSLD
                     SESEEHRETGDHRPTPSGDPSSDAHHDYATGKTSYPPYTAMKVEKEILATLQYYFEMF
                     PISAATASCPVNLFMTTDLTLGQSLMPPLDHIQQTLKASGGHEPQIRITSLSPASHFH
                     LLNTHLAVSHQEEAALPGYASLMVA"
BASE COUNT          829 a          730 c         1023 g          880 t
ORIGIN      
        1 tggactgacc ctcctgcggt attaacggtc aagagttcat tgtcggttgt tcccaggatt
       61 ccgagatcaa tggcgacctg agtcgagagg actgtcatgg aggcaccggt atccacaatg
      121 agttgggccg gtcgttcttg attcagcatg acaggaacga caaatgaccc tccacttttg
      181 gtgaggggta tggtgatttc ctgattaaca ggtctttcct gctcttcctc attcaacgaa
      241 gacacctgag tagaaatctg ggcgtcagga ggtgccgggt caggaatgga cttggggatt
      301 gcttgaagtg ttgcagtcgt ggatgacgct tgattgctga gagaagggct ttgtgttggc
      361 aatagcgtgc agttaaagag ttgggctgga ttgtctgtta aaacgcgtgt tccggattca
      421 tcaaaacagt catatgtcgc tgaatgccct agagacacca atagggcagt gcataccatc
      481 aatgccgaca tccaccaaaa ggtggtgtgg tgaggattcc taagaaacga aacacggcga
      541 cattcataca taatgagaaa agaataccaa gatgcggggg gaaaacgcta tgaaggagat
      601 tatccgctgg tatcgggtgt taagggaatg gtggcctgca catgcgagcc ggtttccaac
      661 acctgggtgg acagaactac ggtttctggt tcgatcttat tcacctgcac gcgaccttcc
      721 aacatctctc cctgtttgac gatatagatg gcctgcccat tggtgaggaa ggcctgactt
      781 tccccccctt tggttaaata ccccaggaac cggaattggt taagttgttc ccgggcccgt
      841 tgccctgcaa gatcggccat agagggacct gggggtgggg gtggtggttg gggcggagct
      901 ggggagggcg ctgttttggg aaggcgagaa gtgggggcgc tgtcaatttt tttttggtgg
      961 gcgacctggg acatgcctag aggtgaaaag atgttgcggg gtagagagaa ttttgcggtt
     1021 tggcgaggat tagaaaatgc cggatctaac tccagatctt taatcggggg aagcggcact
     1081 cctgacgtgg cagatgaagg tagggtatct gttggggcgg aatcccacat ttggaagatc
     1141 gtcaccattc cccaggccaa taataagcct cctaacacca gccattttcg ttgattcgtc
     1201 attaggcttt tgttttttta accggttcag ggaattcttt cagaaaggtg gagaccttca
     1261 gtttaaagat aacccctttc tgttttttag atgtttcaac ggacagtttt tcgataaaga
     1321 gatagggcca gcgttgctcc agttcgaaaa tgaattttcg aatagcctca taggggcctg
     1381 cggcttcaaa cgcaaacgtg ccttttgtgg ccagtttatg ggcgaggggt ttgaagtcgt
     1441 atcccatgcc aggaatttgt acgtgatttt gtttcgccaa atcggcaatg tctaaactta
     1501 aatctgtgaa ttcaccggaa tccggaagag cactccacat ggtggataga atggtttggg
     1561 tctgccgtgc tgtctgcatg gttgcctgtg tatgcaagga cgcttgattc agggccgtga
     1621 tgtgttgaaa acggtcctgg gccgggtaaa agaggaaggc aaacatcccg accgatgaca
     1681 atccggcaag gaccgtcacg ataaacagcg gacctaaata ttttgaaatc cggatcgtcg
     1741 tcttggatcc ttgtatgatc tggaaagaat tcatcgttgt ctgggaaccc ctgtcaggtg
     1801 agaagttggg tgtcgggcat cataggtgac ggtgagcgaa aatacgctat gtgagccacc
     1861 ttgctcctct ttggtcgttt ttgtggaatg ctgcgatagc aacacattct gaaaggctgc
     1921 gtgcttctct aactggtgaa tgagtcgatt gaggtcgggt aaagatgctg ccgatccttg
     1981 gagaaggatg gtgtcggttt tttcatcgag agacactgag ttcagggtga tatccgaagg
     2041 aatcgctgtt tccagatccg tcaataattg tgtccaggaa aatcccaccc gttcacgaac
     2101 ctgtttcaca aaagtgactt ttgagggaat gcttcgaatg gcctggtgcg aaaggtcaag
     2161 cccggccgcc ctggcttggg tgaccaattg ttggttggca gtctcgacac cggcggcttg
     2221 ttcttccagg acatcaatct gttcatgtaa ggagtttcct tcccaccaga atgaagccgt
     2281 aagggcaagt gcaccggcgg tgagaagggt gagcccgagt tgggacaatt gaagaagagg
     2341 aatgccgggt gcagataaat tcctatgtac acgtgggatc atcatgccac catcagactg
     2401 gcatagcctg gcaatgccgc ttcttcctgg tggctcaccg ccaggtgtgt gtttaataga
     2461 tgaaaatgtg aggccgggga tagtgaggtg attcgaattt gaggctcatg tcctccactg
     2521 gccttaaggg tctgttgaat gtggtcgagg ggcggcataa ggctctgccc cagggtcaga
     2581 tcggtggtca tgaacagatt aaccggacaa gatgccgtgg cagcagatat gggaaacatc
     2641 tcgaaataat attgcagtgt ggccagaatt tccttttcca ccttcatggc ggtgtacgga
     2701 gggtaagaag tctttcctgt agcgtaatcg tgatgtgcgt cggaagaggg atctcctgac
     2761 ggtgtcggac gatgatctcc ggtctccctg tgctcctccg actcagaatc cagtgaagga
     2821 acttgaagat catgggcctc cgttttgatg gcaagagctt tcgtacgaat gaagcgtggg
     2881 ctgccatcct gaaaggccaa aaatgtaaat ccccaatgac tgatgaagag gaacatgaat
     2941 gttgggggat gagaggtcca agaagagttt tcagccgtga gtctgtcctg gatgttgggg
     3001 cggtacagat caaagacatc gagccccgac attccaacag aagttgggat gagatctgcc
     3061 tctaagcaca gattttcata ttgttcaata atttcatggc gaatggccgt gcctaacact
     3121 ctgacatgtt ctgtggtatc cggggaaggt gaatctctga ccgacgtggg cacatagagg
     3181 ccaaaggcca gtcgtgattg ggaggtatcc agtttgaggt cctgttgaaa acgccaattc
     3241 accagtgccg tctgctccgg tttttttgtg ggaaaggatg caaaatcaaa aatactcgtc
     3301 cgagcgcata tgtccgggag actgagtgct atggaaattg ggcgacgata gggcttggcc
     3361 agtgccttga gttgagtaag aaactcatcg gtattggcaa tgttgagttt ggtcgaagag
     3421 agcgtcaggc cccccgggga gagcgggagc ctggcgatgt gt
//
LOCUS       DATIAL010000438         7992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_403715, whole genome shotgun sequence.
ACCESSION   DATIAL010000438 DATIAL010000000
VERSION     DATIAL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7992
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403715"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..988
                     /locus_tag="VLA60_13570"
     CDS             185..988
                     /locus_tag="VLA60_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10436.1"
                     /translation="MDGYRVQSQKRKNTALSLVVVGLMLWTPLSEGTVSAYQEEVIHG
                     GGSITGVMKFAGIVPTPKTYKVTMGSHPEFCQTIADKKGFIHIPKVRVSSDLHLADVV
                     VFLQDVDRGKPLPKEAPILAVDQCQFVPRVIGTLADQNLRMAMRDPILHQIRGWEMLD
                     NGRLPLFHISNLGEGGEATLPLKTRRSSIIKWTCDQHSFMEGWLLLTANPYVTVSDDD
                     GVFRLTDIPAGTHTVGAWHPVLGYQEAKITLISGRQETLELTFVSPSNP"
     gene            1145..2332
                     /locus_tag="VLA60_13575"
     CDS             1145..2332
                     /locus_tag="VLA60_13575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF10437.1"
                     /translation="MKDSKTFISLCLVGFCSFVSYDMVRRPALALFVESLGAGPFLVG
                     LLVAVSTVTGIVLKLPIGLLSDQINRKRLMLGGVLAFAIPPFLYPFTSDLWTLGILRL
                     FHGLATAMFTPLALAMVAELFAKRRGEAFGWYTSATQGGGLLGPMIGGAIVYQYGFAP
                     TFLLAGLFGMLALIFFWMIPQTPTPSRVHQYSVPTLWKEMREGLRRVCRIPPILITSS
                     VEAAKMMGNGTLMAFLPLYGLTIELNAAEIGVLFGVQALTSFIAKPVMGRISDRGARQ
                     PLIFGGLCLCGLMVMVIPQIQGYVLLLAVAGAFGFGEAVVTSSTTALVADYSEGKDLG
                     AGMGLRGTIMDIGHAGGPLLAGLLIHTLGYGGGFFCIGVLLLLTAAFFGVTMMGIKKS
                     VVL"
     gene            2373..2504
                     /locus_tag="VLA60_13580"
     CDS             2373..2504
                     /locus_tag="VLA60_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10438.1"
                     /translation="MNEEMGTGDLIIGIVAAIGVVILVVVFIVVVKNVLLKKDSDIQ"
     gene            2520..3176
                     /locus_tag="VLA60_13585"
     CDS             2520..3176
                     /locus_tag="VLA60_13585"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HSF10439.1"
                     /translation="MFSGIVEEIGAIQGIDKGLAGAKFSILASLTLEDLKLGDSISVA
                     GACLTASQVGDTGFSVDVSTETLNCTTLGTITVGTPVNLERAMKLNARMGGHLVTGHI
                     DGIGLLRARQQDGNSIQLTIEAPEEIIRYCVAKGSITVDGISLTINAVSDRSIAVAII
                     PHTAKVTTMGLKQIGDMVNLESDLIGKYVERLLQASGALPPKPATVINRDYLEKRGLL
                     "
     gene            3192..3365
                     /locus_tag="VLA60_13590"
     CDS             3192..3365
                     /locus_tag="VLA60_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10440.1"
                     /translation="MSQDFPPKPKHPLWRLFAVVTLFVIIIPATLWGIVQLYEIICAW
                     CGCCSAIDRPRTR"
     gene            3426..5324
                     /locus_tag="VLA60_13595"
     CDS             3426..5324
                     /locus_tag="VLA60_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HSF10441.1"
                     /translation="MYSSKLYNPKDFFLTLILAGGVFTFDLLLPRGFAEEMLYTGVVF
                     FATQRLPRRMVYVVAIGCTALTIIGFALTFTSDGAPSSLSWPFRFPIINRAFCIAGIW
                     AITLLALQRRRAIEALRTSEDRFGLVAESIKDYGIVMLDPEGNVASWNAGAAHIFGYS
                     TGEVLGRPHSLFYASLAIDSGEPTNFLTEARASGSVMKEEWLVRKNGSRFWGHVGITV
                     LRDEQDQLHGFAAVMGDLTKRKQEEDVIRALLRLSEKLNSTFVLETLLDELVTEAIQL
                     VQAHAGFAGLASGETLTCEKYIQGRTIKLCHRSWSAGQGLPGWLLLHKTAYLTNHAQE
                     DRQIEQDFRQAFDIHSALSIPILDAKDTLLGCIEVHNKEKASGFTHVDQQTMIGVSQV
                     ASIAIQNALAYQKLQEAEALHSHLLDKIMTAQEDERRRISRELHDEIGQSLTSLLVGL
                     RAAENEACSDGMGARVNDLRKITSTTLQEVQRLAKGLRPSVLDDFGLEEAIARYGAEF
                     SSTYGIEVDVAQNWQSKDRLAPAVETALYRIVQEALTNVGKYAKATTVSILLQQNPAQ
                     IRLIVEDNGQGFDAQAIVRKAAAGAHLGLHGMRERTLLLNGSISIESSAGTGTTIYVN
                     IPLKGEGA"
     gene            5321..5992
                     /locus_tag="VLA60_13600"
     CDS             5321..5992
                     /locus_tag="VLA60_13600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF10442.1"
                     /translation="MKIRIYIVDDHAVLRSGLRLLINGQPDMEVVGESGGAASAIEAV
                     QDLLPHVILMDLAMQGHMHIDTIGELKKCCPSGYILVLTMHTESGYVRGSLAAGASGY
                     VVKSAADTELLTAIRTVAQGKTFLDWTVGKGVGQDPPGAHKKRPADRAPGLLGQLSPR
                     EREIFGLVAQGFTNQHIADQLGISIKSVETYRARVMEKLGLRSRADLVQFALSCGVLT
                     SGNPL"
     gene            6309..7982
                     /locus_tag="VLA60_13605"
     CDS             6309..7982
                     /locus_tag="VLA60_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF10443.1"
                     /translation="MMPMIHGLHFNNLRGDIYGGVVAAVVALPLALAFGVASGAGAIA
                     GLYGAIFVGLFASLFGGTPAQCSGPTGPMTVVMAGILVQFSDNPALAFTVVIMGGGLQ
                     ILFGLLGFGHYIALVPYPVISGFMSGIGCIILILQVGPLVGFEAKPEGIYANLVAIPE
                     FLSNPFPQATILGLLTIAIMYLTPVRMSQLIPPQLLALCAGTLGAYFFFPHAPVIGNV
                     PEGFPSLMIPDMETSVLLLMVEGAVVLALLGSIDSLLTSLVCDNMTRTQHDSNQELIG
                     QGIGNMVAGLFGGLPGAGATMRSVANIRSGGRTPLSGILMSLVLLATLLGLGPLAEKI
                     PLAVLAGILFKVGLDIIDWRFLRHLVKAPRTDVLIMGVVLVITVLVDLITAVGVGIVL
                     ASLLFVKRMAELELANLRVISEPTQEIPFHPEEKAILERNNGRIVLIHVDGPMSFGSA
                     KTMVRRLESIKGFNTFTHVILDMTDVSAIDGTAALAVEDMLRMVQAHRQNLYFVGIKP
                     GVLKVLEGLGVLDHIGPEQRYDLRLDALRHADQGHNFLPTDELSGVQRA"
BASE COUNT         1823 a         1832 c         2182 g         2155 t
ORIGIN      
        1 aagggcggaa atcactaaag agaaaccgaa gtgttgagga attaaagatg aggcccaccg
       61 ggaactgaat attcactgtt ccccgaattg tgagtagatt ttattttcca gtctagtcat
      121 ttccctggtg tcggtagtcc ggattgacta ttaaggacaa atttttagga aggagatgtg
      181 tgtcatggat gggtatcgcg tccaaagtca gaaaagaaag aatactgccc tatccttggt
      241 cgtagtgggt ttgatgttgt ggactccgct gagcgaaggg acggtgagtg cctatcagga
      301 agaggtgatt cacggtggag ggtcgatcac tggtgttatg aaatttgctg ggattgttcc
      361 aacacctaaa acctataagg tcaccatggg aagccatccc gaattctgtc agactattgc
      421 ggacaaaaaa ggtttcatcc atatcccgaa agtccgtgtg tcttccgatc tgcacttggc
      481 ggatgtggtc gtttttctcc aagatgttga ccgtgggaaa cctctcccga aagaagcgcc
      541 gatcctggcg gtggaccaat gtcaattcgt gccacgagtc atcggcacct tggccgatca
      601 aaatctccgt atggccatgc gcgatcccat tcttcatcag atccgggggt gggagatgtt
      661 ggataacggc cgtctgccgc tttttcatat ttccaatttg ggagagggtg gggaagccac
      721 tctcccactc aaaacacgcc gtagcagcat tatcaaatgg acatgtgatc aacacagctt
      781 tatggaaggt tggcttctgt tgaccgcgaa tccgtatgtg acggtatccg acgacgacgg
      841 ggtttttcga ttgaccgata ttcctgcggg cacacatacg gtcggggcct ggcacccggt
      901 gctgggttat caggaagcca agataacgct catttccggg cggcaagaga ccctcgagct
      961 gaccttcgta tccccctcga acccatgagg cgtgctgagt caatcggcgg ctgatcaggc
     1021 agtcgatgaa tttcttaaag actatcgcgt gtaagatgtg acttttaaaa ctttcgtctt
     1081 ccttctcaga atctcgcaat gaccgttttc tctcaccggc tctccttttc gtttggtctc
     1141 taggatgaaa gattcaaaga catttattag tctttgtctc gtcggttttt gtagttttgt
     1201 cagttatgac atggtgcgtc gaccggcctt ggctctgttt gtggaaagcc ttggagccgg
     1261 cccatttctg gtcggcctct tagtggctgt ctcgacagtg accggcattg tactcaagct
     1321 tccgatagga ttgctctccg accagatcaa tcgaaaacgg ttgatgttgg gaggggtgtt
     1381 ggcctttgcg attccgccgt ttctttatcc gttcacctcg gatctctgga cgttaggtat
     1441 tctccgattg tttcatggat tagcgaccgc gatgtttacg ccgctggcct tagcgatggt
     1501 ggcagagctc tttgcgaaac ggcgggggga ggcttttggt tggtacacct cggcaaccca
     1561 gggcggtgga ctgctgggtc caatgattgg gggggctatt gtctatcaat atggatttgc
     1621 ccccacgttt ctcttagccg gattatttgg aatgttagct ttaatcttct tctggatgat
     1681 cccccagacg cccacaccct cgcgggttca ccaatattcc gtcccgacac tatggaagga
     1741 gatgcgcgaa ggcctccgtc gagtctgccg gattccaccc attctcatca cgagtagcgt
     1801 ggaagctgcc aagatgatgg gcaacggcac gctgatggcg tttttgccgc tctatggatt
     1861 gacaatcgaa ttgaacgccg ccgaaatcgg ggttttgttt ggagtgcagg cgttgacttc
     1921 gtttattgcc aaaccggtga tggggcgaat ttctgatcgg ggggccaggc agccgttgat
     1981 ttttggaggg ctgtgcctgt gcggcctcat ggtcatggtt attccccaga tccagggcta
     2041 tgttctctta ttagcggtcg ccggagcgtt tggatttggt gaagcggtgg tgacgtcctc
     2101 gaccactgcc ctggttgctg attattcaga aggaaaagat ctgggagctg ggatgggatt
     2161 acggggaacc ataatggata tcgggcatgc aggcggaccg ctcctggccg gcctactcat
     2221 ccatacctta ggctatggag gagggttttt ctgtattggg gtcctcttat tactgacggc
     2281 ggcatttttt ggtgtcacaa tgatggggat caaaaaatca gttgtgctat aatgggcttt
     2341 ttcctgaagg gcaggcaaag gagtcgaacg ccatgaatga agaaatggga accggtgatc
     2401 tcatcatcgg gattgtggcg gccatcggtg tcgtgatctt agtggtggtc tttatcgtgg
     2461 tcgtgaaaaa tgttctttta aagaaagaca gcgatatcca ataaccaaat aggtgggaca
     2521 tgtttagcgg aattgttgag gaaatcggtg cgattcaggg aatagataaa ggattggctg
     2581 gtgcaaaatt ttccattttg gcttcgctga ctttagaaga tctcaaactg ggagatagta
     2641 ttagtgtggc cggtgcctgc ttaacggcaa gccaggtggg tgacacgggt ttttcggtgg
     2701 atgtgtccac cgaaacattg aattgcacaa cattaggaac cattactgtc gggacaccgg
     2761 tcaatttgga gcgtgccatg aaactcaatg ccaggatggg gggacatctg gtcacaggcc
     2821 atatcgatgg cattggtctc ttgcgtgctc gacagcagga tgggaattcg attcaactca
     2881 ctattgaagc tccagaggaa attatccgct attgtgtggc gaaaggctca attaccgtgg
     2941 atggaattag tttgaccatc aatgccgtgt cggatcgttc tattgccgtg gccatcattc
     3001 cgcataccgc caaagtgacg accatgggcc ttaagcaaat aggggacatg gtcaatctgg
     3061 aatccgactt aattggaaag tatgttgaac gcttattgca agcaagtgga gctctcccgc
     3121 caaaacccgc cacggtgatc aatcgagatt atcttgaaaa acgaggcctg ctatagtttt
     3181 tttcacattc catgtcacaa gattttccac caaaacccaa acatcctcta tggcgattat
     3241 ttgccgtcgt cacgctcttt gtgatcatca ttcccgcaac gttatggggt atcgttcaac
     3301 tctacgaaat catttgtgcc tggtgcggct gctgtagtgc catcgatcgt ccacgcacca
     3361 ggtagatttt cactcaggca tttactgcca tcgtcaatga atgaggagaa cgggtttttc
     3421 tccttatgta ttcaagcaaa ttatacaacc ccaaagattt tttcttaacc cttatcctgg
     3481 ctggtggggt tttcacgttt gatctcctgc tcccgcgagg atttgcggaa gaaatgttgt
     3541 acacaggggt ggtgttcttt gcgacccaac gtttgccacg aaggatggta tatgttgtgg
     3601 ccattggatg caccgctctc acgatcatag gctttgccct cacattcacc tcggatgggg
     3661 caccttcatc attaagttgg ccattccggt ttcccatcat caacagagcc ttttgtatcg
     3721 ccggtatttg ggccattacc ctcttggcac tccaacgaag gcgggcgata gaagctcttc
     3781 gaacgagtga ggatcgattt ggtttggtcg ccgaaagcat caaggattat ggaattgtga
     3841 tgctggatcc ggaagggaat gtcgccagtt ggaatgccgg tgcggcacat attttcggct
     3901 attccacggg agaggttctg gggcggcctc atagcctgtt ctatgcatcc ttagctattg
     3961 actccggcga accaaccaat tttctgactg aggctagagc ttcggggagt gttatgaagg
     4021 aggaatggtt ggtacgcaaa aacgggtcca ggttttgggg gcatgtgggc atcaccgtct
     4081 tacgtgatga acaggatcaa cttcatgggt ttgccgcggt gatgggggat cttaccaagc
     4141 gaaagcagga agaagatgtg attcgagccc tgcttcgtct gagtgaaaag ttgaattcca
     4201 ctttcgtgct ggaaacgctc ctggacgaat tggtgacaga agccattcaa ctggtccagg
     4261 cccatgccgg gtttgccggg ttggcctcgg gggaaacgtt gacatgtgaa aaatatattc
     4321 aaggtcggac aataaaactc tgtcatcgtt cctggtctgc ggggcaggga ttacccggct
     4381 ggctgctcct gcataagact gcctatctta cgaatcacgc acaggaggac agacagattg
     4441 agcaggattt tcgtcaagcc tttgatattc actcagcctt aagcattcca attctcgatg
     4501 ccaaggatac tcttttggga tgtattgaag ttcataacaa agagaaggcc agtggcttca
     4561 cacatgttga tcagcaaacc atgattgggg tttcccaggt ggcctctatt gccattcaga
     4621 atgccttggc ttaccaaaaa cttcaagaag ctgaggcgct tcatagccat ctgttggaca
     4681 aaatcatgac ggctcaagaa gacgagcggc gtcgaatcag tcgggaatta catgatgaaa
     4741 tcggccaatc tctgacttct cttctggttg gacttcgtgc ggctgagaat gaggcctgtt
     4801 cagatggaat gggagcacgg gtgaatgatc ttcgaaagat tacgagtacg acactgcagg
     4861 aggttcagcg actggcaaaa ggtctgcgtc caagcgtact ggatgatttt ggactagagg
     4921 aagccatcgc ccgctatggg gccgaattta gttcaaccta tgggatagag gtggatgtgg
     4981 cgcaaaactg gcaaagtaag gatcggcttg ctcccgcagt ggagaccgcg ttgtatcgaa
     5041 ttgttcagga agcgctgaca aatgtcggca aatatgccaa ggccaccacc gtgtcaatac
     5101 ttctacagca aaatccggca cagattcgac ttattgtgga agataatggt cagggatttg
     5161 acgcgcaggc gattgttcgg aaggctgcgg caggtgcgca tcttggcctg cacgggatgc
     5221 gggagcgaac cctgttgctc aatggttcga tttccattga atcatctgcc ggaaccggaa
     5281 ccaccatcta tgtcaacatc cccttaaaag gagagggggc atgaaaattc ggatttatat
     5341 cgtcgatgat cacgcagtcc tccgatcggg attgcgtctc ttgattaatg ggcaaccgga
     5401 catggaagtc gttggtgaat ccggaggcgc cgcttccgcc atagaggccg ttcaagatct
     5461 gttgcctcat gtcatcttaa tggatctggc catgcagggt catatgcata ttgacacaat
     5521 cggcgaactg aaaaaatgtt gtccttccgg atatattctt gttctgacca tgcacaccga
     5581 aagtggctac gttcgtgggt cattagctgc cggcgcttcg gggtatgtcg tcaagagtgc
     5641 ggccgacacc gaattattga cagcgatccg taccgtggca caagggaaga cctttctgga
     5701 ttggactgta gggaaagggg tggggcagga cccccccggg gcccacaaaa aaaggccggc
     5761 tgacagagcc cctggattgc tggggcaact cagtccgaga gagcgcgaga ttttcggtct
     5821 ggtcgcacaa gggtttacca atcagcacat tgccgatcaa ttggggatta gtatcaaatc
     5881 ggttgaaacg tatcgtgcaa gggttatgga aaaactcggt cttcgtagtc gtgccgacct
     5941 tgtgcagttt gcgctgagct gtggtgtgct gacgtccgga aatcctctct aaactcccac
     6001 cttttccccg atagagcaat gttccaaaca gcctcgtatg gtcttgtcag gttttcccct
     6061 ctgccatttc atccgtattc ccgttaattt aatccagtaa gagcacaaca ctcaatctca
     6121 taactctttt attccatttt gcccatattt cttttagcaa caggcacggt gaaggctaat
     6181 ctatcataaa gattatggtc attttcttct ccgatgcacc ccatacataa aacacggcag
     6241 caggcaggtc ccctgatttg ccgaaaataa tagtatcgag ggatttcatc cctagaggag
     6301 taagagagat gatgcccatg attcatggac tgcatttcaa taatcttcga ggagatatct
     6361 acggtggcgt ggtggcggct gtggtcgcac taccattagc cctcgctttt ggggtggcct
     6421 caggggccgg tgcaattgcc ggactctatg gcgctatttt tgtcggattg ttcgcatccc
     6481 tattcggggg gacccccgcg caatgttccg gccccaccgg tcccatgacg gttgtgatgg
     6541 ctggaattct ggttcaattc tctgacaatc ccgccctcgc atttaccgtg gtgatcatgg
     6601 gtgggggatt acaaattctt tttggattgc tcgggttcgg gcattatatt gcccttgttc
     6661 cctatccggt gatttcagga ttcatgagcg ggattggatg cattattctt attttgcagg
     6721 ttggaccgct tgttggattc gaggccaaac cggagggaat ttatgccaat ctggtggcca
     6781 ttccggaatt tttatcaaat cctttccccc aagcgaccat tctgggtctc ctgactattg
     6841 cgatcatgta tctgacacca gtacgaatga gtcaattgat tcctccccaa cttctggccc
     6901 tatgtgcagg caccctgggc gcctatttct ttttccctca cgcaccagtg attggaaatg
     6961 ttccggaagg ttttccgtct ttgatgatcc ctgatatgga gaccagtgta ttgctcctga
     7021 tggtagaggg ggcggtggtg ttggctctcc ttggaagtat cgatagtctg ctcacctcct
     7081 tggtgtgtga caacatgacc cgtacccaac atgattcgaa tcaagaattg attggtcagg
     7141 gaattggcaa tatggtggcc ggtctatttg ggggccttcc gggagcaggc gcgaccatgc
     7201 ggtcggtggc caatattcgg agtggcggcc gaacgcccct atcgggaatc ctcatgtcat
     7261 tggtgttatt ggccacattg ctgggcctgg gaccgttagc ggaaaagatc cctttagccg
     7321 tgttagccgg gattttattc aaggtggggt tggatattat cgattggaga tttctccgac
     7381 acttggtcaa ggccccacga accgatgtgc tgatcatggg ggtggtgctg gttattacgg
     7441 ttcttgtgga tttaatcacg gctgttggcg tgggaatcgt cctggcgagt ctgttatttg
     7501 taaagcgcat ggccgaactt gaattggcca atttacgggt cattagtgag ccgacacagg
     7561 aaatcccgtt tcatcctgaa gaaaaagcca ttcttgaacg gaataacggt cgaattgtct
     7621 taatccacgt agatggcccg atgagtttcg gttccgcgaa aacaatggtg cgacggctgg
     7681 agtcaatcaa ggggttcaat acctttacgc atgtgattct tgacatgact gatgtgtcag
     7741 ccattgacgg aaccgccgct cttgcggttg aagatatgct tcgcatggtt caggcacatc
     7801 gccagaattt atactttgtt ggaattaaac cgggagtcct taaagttctg gagggattgg
     7861 gggttttgga tcacattgga cctgaacaac ggtacgacct tcgattggat gcgttgcgtc
     7921 atgccgatca agggcataat tttttaccga cagatgaact ctccggcgtg caacgggcat
     7981 gaccaaaata tt
//
LOCUS       DATIAL010000439         3849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1478894, whole genome shotgun sequence.
ACCESSION   DATIAL010000439 DATIAL010000000
VERSION     DATIAL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478894"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VLA60_13610"
     CDS             complement(<1..1032)
                     /locus_tag="VLA60_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF10444.1"
                     /translation="MSDSSEFSSSKDSSRSGGSSFPKSSRKSGNSPLAVIDQCLASLP
                     EGDHRIPLLYQLRHGLVEQTTASQQQEADMKKLQGVLEKLTAPANRVGTLLDQPDKGV
                     ARIMVGGAEYYANVDPRLEEEGLKVGHQILVNEAYVVIRSLGYDRNGPIVKVREILVD
                     GRLRIDQEAGRQGILIQRADDLAGTELKVGDEVRLDPSHRIAIERLENPQGQSHLLAE
                     LPTVRWEHIGGQQEAIQAIRRSIEYPLLHGELFSRYQFQQPKGFLLYGPPGCGKTLIG
                     QATAASLGQLFAKERETLTPHVGTQGQGSASQLPPIEGGVFLHVKGPEVLNMWVGESE
                     RIIRDLFLQA"
     gene            complement(1235..1564)
                     /locus_tag="VLA60_13615"
     CDS             complement(1235..1564)
                     /locus_tag="VLA60_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSF10445.1"
                     /translation="MGSKIYVGGLPYSTAEQELADLFGQHGAVTSAKIITDKYTGQSR
                     GFGFVEMASDAEAKAAIEALNGTEMGGRTLTVNEAKPQAPRTGFGGGGGGNRGGGGNR
                     GGGGDRW"
     gene            complement(1845..3125)
                     /gene="clpX"
                     /locus_tag="VLA60_13620"
     CDS             complement(1845..3125)
                     /gene="clpX"
                     /locus_tag="VLA60_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HSF10446.1"
                     /translation="MAFWDSKQGRNRADAVCSFCGRAKPGATNLIQAPTKQACTQCQA
                     PGSVLICADCIQQCSKFLSGHTEGQSAIEPPAGPLPTPPEIKEVLDQYVIGQDRAKKV
                     LSVAVHNHYKRVFSGKDLPDVELEKGNVMLIGPTGTGKTLLAKTLAKILHVPFAIADA
                     TTVTEAGYVGEDVENVVLKLLQSCDFNMARAQVGIIYIDEIDKISRKSESASITRDVS
                     GEGVQQALLKLVEGTLCNVPPKGGRKHPEQEFLRVDTTNILFICGGAFVGVEDIIAQR
                     TNQKRMGFGAGSSNGETLSSDQLMEKLKPEDLLKYGLIPEFVGRFPILASLNDLRVED
                     MVRVLQEPKNALLKQYQALFQLDGVQLDFTPEAVRAIATRASSMKTGARGLRSILEEV
                     MLDLMYDVPALKNIKRVIISEETIDGRGAPQLIS"
     gene            complement(3248..>3849)
                     /locus_tag="VLA60_13625"
     CDS             complement(3248..>3849)
                     /locus_tag="VLA60_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10447.1"
                     /translation="GGETWSKRMEGMKEVLMVVTLDCDPRQPQTLYAGTSGGVYKSID
                     GARTWKMVNNGLVAAEVLKSSRALSVTRIKVDPHESDTVYTATLSGLYVTKDGGQVWT
                     RIGETLSDQMLSDLILDHTYPHIVYVTNREGVFRSEDGGITWEARSAGLTNVNIRALA
                     MSAVDSDVLYVGTNGKGLFRTGDRGMSWEAVPLVVDESL"
BASE COUNT          963 a         1108 c          837 g          941 t
ORIGIN      
        1 ggcttgaaga aataagtccc ggatgatgcg ctccgattcc ccgacccaca tattcaggac
       61 ttccggacct ttgacatgca gaaacacgcc accctcaatc ggaggaagtt gagaggcaga
      121 gccttgccct tgcgtgccaa catggggcgt aagcgtttct cgttctttgg cgaagagttg
      181 cccaagactt gccgccgtgg cttggccaat cagggtcttt ccgcaccccg gagggccata
      241 caggagaaag cccttgggtt gctggaattg ataccgtgag aacaactctc catgcaggag
      301 gggatattct attgaccggc ggatggcttg aatagcctcc tgctgtccac cgatatgttc
      361 ccaacggaca gttggcaatt ccgctaaaag atgcgattgc ccttgggggt tctctagtcg
      421 ttcgatggca atcctgtgag atgggtccag tcgaacctca tcgccaacct tcaattccgt
      481 accggcaaga tcatccgccc gttgaataag aatgccctgc ctgcccgcct cttgatcaat
      541 acgaagacgc ccatcaacta atatctctcg aactttcaca atcgggccat tccgatcata
      601 cccgagagac cggatgacca catacgcctc attcactaaa atctgatgcc caacctttaa
      661 accttcttcc tcaagccttg gatcgacatt ggcataatat tccgccccgc caaccatgat
      721 cctcgccaca cccttatcag gctgatcaag cagggtcccg actcgatttg ccggggctgt
      781 cagtttctca agcactcctt gaagtttctt catatcggct tcctgttgtt gagaagccgt
      841 cgtttgttca acaagcccgt gtcgaagttg atagagaaga ggaatccggt gatccccttc
      901 aggcaaagat gccaaacatt ggtcaatgac cgccaacggg gaattcccag atttccggga
      961 ggatttcggg aatgaagatc cccccgatcg agaagaatcc ttggaggaag aaaattctga
     1021 agaatcgctc attgtgtatt gtcttccatt ttatttcaat tgggacatga aagccgcgat
     1081 gcccatcaat actcccgacg agaaatcttg gccgataatc agtatctcat tctacaatgt
     1141 ttccggacca ttacccaagg tataaaaaaa gcaggggatc cgttatcgga tcccctgctt
     1201 ttattgaagt gtagcctgct agtgacttag cgaattacca acggtctcca ccgccaccac
     1261 ggtttccgcc accgccacga tttcccccac caccgccacc aaatccggtt cgcggtgctt
     1321 ggggtttcgc ttcattgacc gtcagggtgc gccctcccat ttcggttcca ttcagcgcct
     1381 caatcgccgc cttggcttca gcatccgagg ccatttccac aaaaccaaat cctcgagatt
     1441 gccccgtgta cttgtccgta ataattttag ccgacgtcac ggcgccgtgc tggccaaaaa
     1501 ggtcagccaa ttcttgctct gccgtggaat acggcaggcc acctacatag atctttgaac
     1561 ccatcggggt ccctccttct aaatgcgata ttgttttgca tgggtcgaag agaagagaag
     1621 ggaaggaacg gcccgagaac gcaaaccagg tggcgactta ggactaactt ccaatcagac
     1681 tccttgcgga ttccttggca aaacaacatc agtgatgggc ccgtcactta accattcacc
     1741 acgtggcccg aatgtcgtga tgaatttctt tggattagca tatgtcagaa tgaaaaagaa
     1801 tgcaacaatc cccggcaagg aaactactcc tccgagcact attttcagga aattaattgg
     1861 ggagcccccc tgccgtcaat cgtttcctcg gagataatca ctcgcttaat gtttttcaag
     1921 gcgggcacat catacatcaa atccagcatc acttcctcca gaatagaacg caatcccctg
     1981 gctccggttt tcatagacga agccctggtg gcaattgctc gaaccgcctc cggcgtgaaa
     2041 tccaactgca cgccatctaa ttgaaataag gcttgatatt gtttcagaag agcatttttc
     2101 ggttcctgta gaacccgaac catatcctcc acgcgcaaat cattcagaga ggccagaata
     2161 ggaaaccggc cgacaaattc agggatgagt ccatacttta aaagatcctc cggcttaagc
     2221 ttttccatca attgatcaga gctcaatgtc tcgccattgg aggaacccgc gccaaaaccc
     2281 atgcgtttct gattggtccg ctgagcaata atatcctcca cccctacaaa cgcaccgcca
     2341 caaataaaga ggatattcgt cgtgtccacc ctgagaaatt cctgctcagg atgctttctt
     2401 cctcccttgg ggggcacatt gcataacgtc ccttccacca atttcaacaa cgcctgttgg
     2461 acaccttccc cggacacatc ccgcgtgata gaggcgcttt cagacttccg gctaatctta
     2521 tcaatttcat cgatataaat gatgcccact tgcgctctgg ccatattaaa gtcacacgac
     2581 tgcaataact ttaagaccac attttccaca tcttcgccca catatcccgc ttccgtcacc
     2641 gtggtagcat ccgcgatggc aaaggggaca tgcaaaattt tcgctaaggt tttggctaat
     2701 aaggtcttgc cggttccagt cggcccgatc aacatcacat tccctttttc caattccaca
     2761 tcaggcagat ctttaccgct aaagacccgt ttataatgat tatggacggc aaccgacaga
     2821 acctttttgg cacggtcttg tcctatcaca tactggtcca ggacctcttt gatttcagga
     2881 ggggtcggca agggacccgc tggcggctcg atggcggact ggccttccgt atggcctgat
     2941 aaaaattttg aacattgttg gatacaatcc gcacaaatca agaccgaccc cggtgcctga
     3001 cattgcgtgc aggcttgttt cgtcggggcc tggatcaaat tggtggctcc aggttttgcc
     3061 cgaccacaaa aggagcagac cgcatccgca cgatttcgtc cttgcttcga atcccaaaaa
     3121 gccacggcag ttacctttct tactcgtcac cagaaatcag tctaaaagac tctatcactt
     3181 acccatgaaa gaagatctta atcttcaaga agaaggatat caaatcatct cccattcagg
     3241 ataaacgtca aagggattcg tccacaacca ggggcacagc ctcccatgac attccacggt
     3301 cacccgtccg aaagagtcct tttccatttg tccccacata aagcacatcg gaatcaaccg
     3361 cgctcatagc taaagcccga atattaacat tcgtcaagcc cgcacttcga gcttcccagg
     3421 ttattcctcc atcttcactt ctaaagacgc cttcacgatt ggtcacatac acgatatggg
     3481 ggtaggtgtg atcgagaatg aggtcactca acatctgatc ggagagtgtc tccccaatgc
     3541 gagtccagac ttgccctcca tccttggtga cgtacagtcc actcagagtc gcagtataca
     3601 ccgtatcgga ttcatgaggg tcaaccttaa ttcgagtcac gctcaatgcc cgtgaggatt
     3661 tcagcacctc agcggctacc aacccattat tgaccatttt ccaggtgcga gccccgtcaa
     3721 tggatttata gactcccccg cttgttccgg catagagcgt ttgaggctgt cggggatcgc
     3781 aatcaagcgt cacgaccatg agcacttctt tcatgccctc catccgctta ctccaggttt
     3841 ctcccccat
//
LOCUS       DATIAL010000440         8790 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1478950, whole genome shotgun sequence.
ACCESSION   DATIAL010000440 DATIAL010000000
VERSION     DATIAL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478950"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..719)
                     /locus_tag="VLA60_13630"
     CDS             complement(27..719)
                     /locus_tag="VLA60_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HSF10448.1"
                     /translation="MIACVTFDFDGTLVDSNQVKVQSFYKIVENYDPSGDTVTEVLQR
                     CSHKDRYGITRELAREFMAKGLIPPHTGTEILGLQWAETYTTTCQAAVVGCPEVPGAS
                     GILSWLLNQNIPLYLNSRTPTKALNRLVTLRNLTQYFSGIYGAPASKLENLLHIQKLT
                     QAKPEEILFVGDSEDDWNAAAEFGCHFAGVILRGNSRFTQIPPLHMTNLAEIRTIVKN
                     LQENRNDFAPNH"
     gene            complement(716..2299)
                     /locus_tag="VLA60_13635"
     CDS             complement(716..2299)
                     /locus_tag="VLA60_13635"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HSF10449.1"
                     /translation="MIDQDFNFHDLFILDLANNHQGSVEHGLGIIQSTAEVIKRHQVR
                     AAIKFQFRQLDTFIHTDHQSNSELKYIQRFQSTRLDQIQFQTLLNEVWAQGLLAMCTP
                     FDEESVNIAVDMGFNVLKVASCSAKDWPLLEEIAGAGPPVVCSTGGLTLEDIDNVVSF
                     FQHRAVQFALMHCVSVYPTPDPLMNLNQIQILRNRYPNIPIGWSTHENPGDTVPVQIA
                     VALGARLFERHIGLETESIKLNAYSSTSQQVDTWLEAYHRAKELCGPKTRPPASEVEQ
                     SSLDGLRRGVFAKRSIKKGRELTRDLVYFAMPYVEGQMESGAWKEGYTAVQDITPDQP
                     VMRDAVEITLNQGLVTLKQAIHEVKALLNEANIQLGSEFKVEYSHHYGLENFRQTGAV
                     LIECINREYCKKLVIQLPGQRHPSHYHAKKEETFQILFGILHVNIDGYPRILHPGETI
                     LILPGVWHSFWTDTGVVFEEVSTTHYNNDSFYADKRINKLQRSERKTMVDHWGRFQIA
                     QQAASEKTPEVPLPDPHAQ"
     gene            complement(2324..3415)
                     /locus_tag="VLA60_13640"
     CDS             complement(2324..3415)
                     /locus_tag="VLA60_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSF10450.1"
                     /translation="MYNVKIFGAGSIGNHLAHASRSLGWNVMICDVDQAALDRTKQTI
                     YPTRYGKWDEAIKLSLVKDAPRGGFDLVVVGTPPDSHLSLALGALEEKPKAILVEKPL
                     CPPNLEQAQALQKMVVESGTFAFVGYDHVVGKAAQETLQAGRQLSTIETIDVEFREHW
                     GGIFAAHPWLQGPQDSYLGFWKRGGGASGEHSHAINLWQFLSGGLGKGRITEVSATLD
                     YVQTDMVEYDKLCLLSLRTEAGLLGRVVQDVITSPSRKWARIQGNDGFAELTIGHQPG
                     VDRVDWKTGGDDTQFRTIQKTRPDDFIWELQHIEGVMTGKTKDSVLSLERGLETMLVV
                     AAAHLSSAKKRTVRIDYRKGYTPEALELL"
     gene            complement(3510..4901)
                     /locus_tag="VLA60_13645"
     CDS             complement(3510..4901)
                     /locus_tag="VLA60_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface carbohydrate biosynthesis protein"
                     /protein_id="HSF10451.1"
                     /translation="MTKPRRRLTIPVEIFNREFDAKVLLSCFATERGFSVIIGEKHEI
                     QLNLAALPKSIYLPTNLHNRNAIVFSLLAQLGHSLVGTDEEAIVYCSPEVYVKEKLGS
                     TEFSRPNLFLAWGPENARIWKSQPAYNGMPVHITGNSRLDLLRPEMRQYWTPQVETIH
                     KKFGKNILINTNFGKLNHFRPNKGSEKDALERAAVSPSDVDEFELGMARHRLALFQYF
                     QEMVGQIAAAFPAHTILIRPHPSENHETWRQAANGYTNVQVHIEGHVIPWLLAADAII
                     HNSCTTGLEGYVLKRPVFSYQPIISDRFDKQLPNAVSHRATSARELIELIKATLSGDQ
                     SPQENGSEAKTRLIEQYLAALQGPFASENIVSTLETFDATCSPSTPKWGTVFAAKSQA
                     LLRKLRRRFNAQFRMGDQNRSQRYNYLNHIFPDITLGHIEERIERLQHALGRFHHTQV
                     RPLQDNIFEIFPN"
     gene            complement(4964..7342)
                     /locus_tag="VLA60_13650"
     CDS             complement(4964..7342)
                     /locus_tag="VLA60_13650"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HSF10452.1"
                     /translation="MEVREGTASSGKAPVRIFVGSESAQGRAERILIWSIEQVRDPGR
                     VYEIYLMKELKGFDRRKWLTGFTNYRFAIPAFAGQAGRAIYNDVDQIYLADPGLLFDH
                     DLNDHGFLSIARNDTSVMVMDCKKMAAIWSLEAAKFKKKNALVQRALEVPNLWGPLEP
                     EWNARDEEYQAGQSKVLHYTALHTQPWQPFPQEFVYQKSLEGDVWFSLEHSANQKGFL
                     VFSRKHPSSLFTELANETRDLRNISPTKLSPFQDVETINELQEAVRTLQAKSLLYCHF
                     GQLFENVPEHLRATDLSSLTMTTMDLCQEDSGHLPSRQFDLVLSPGGLHLVPEEDIPW
                     VIEEMFNRAHGMVYMAIEITNPDQSYGAENPHACLKQNINRWMIHLESIGARYPAIHW
                     KLAFHAKTPKGFQQIFIRCGGHWIGSPPRIWILSDGKVGHTTQSEVLAKSLGWPYEIK
                     QLRFYGWHKIQKLVWGLFPPNLIGVDRVHSSPLCPPWPDLVISTGWRPAPIARWIREQ
                     SQGKTRIIQLGRKGGCSADLFDVVITPSYYGFPPHPHRIETVAPLNELTQDSLARVKQ
                     RWPNLFDNAPHPRIVFVVGGATARFKFTPDMARTIGGQLKHLAQKCHGKIFAVTSPRT
                     GKDLIHALESTLLPDHFVHTWKPNQSDNPYFAYLADADVIIVTGESESMLAEAASLGK
                     PVYIVPLPENSPTWKVRLSEKIIQRAQSQPLNKRGTARPQQGLERLCARLIRAGLVQP
                     RRNLTMLHDSLIKNGIARPFGEPITNGTRPQLKETETVARKVKATLGIFDHL"
     gene            complement(7461..8690)
                     /locus_tag="VLA60_13655"
     CDS             complement(7461..8690)
                     /locus_tag="VLA60_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF10453.1"
                     /translation="MSLFDKFLPIQEARNELLKSGVNPFHVTVEKIISPTEAIINGRK
                     TILVGTNNYLGLTFHPDSILAGQQALAQEGTGTTGSRMANGSFASHKALENAIADFYG
                     APYAIVFSTGYQANLGVLSALAGPGDTILIDGDSHASIYDGCRLGGAEIIRFKHNDVA
                     DLEKRLRRLGDRSYRTLIVVEGIYSMLGDRAPLAEIVEVKRKYGATLIVDEAHSLGVL
                     GQHGRGLAEETGVEQDVDCIVGTFSKSLGSTGGFGVSFHPEFELIRYTSRPYIFTASP
                     CPSVVASTRATIRIIQAHPELRQHLWTNSRHLYEGLTKSGYILGAEVGPIIAAMVPNK
                     ELALAMWQQLLENGIYVNLMIPPATPNGECLLRCSVSAAHTHNQIEKIISAFADLPHG
                     TQTHSNPAHLQPDATLN"
BASE COUNT         2060 a         2048 c         2303 g         2379 t
ORIGIN      
        1 cccgattaaa tggtgcgggt agatgctcaa tggtttgggg caaagtcatt tcgattctcc
       61 tgaagatttt tcacaattgt tctgatctca gccaggtttg tcatatggag tggtggtatc
      121 tgtgtgaatc ggctgttacc acgaagaatg actccggcaa agtggcagcc aaattcagct
      181 gcagcgttcc agtcatcctc actatctccg acaaagagta tttcctctgg ttttgcctga
      241 gtgagctttt gaatgtgaag caaattttcg agtttacttg cgggagctcc atatattccc
      301 gagaaatact gggtgaggtt tcgtagggtc acaaggcgat tgagggcttt ggtcggcgtt
      361 cttgaattga ggtatagagg aatattttga ttcaacagcc atgagagtat ccctgaggcc
      421 ccagggactt ccggacatcc gacaacggcc gcttggcagg tggttgtgta agtctcggcc
      481 cattgtaaac cgaggatctc tgtgcctgtg tggggcggaa taagcccttt tgccatgaat
      541 tcccgggcaa gttcacgggt gattccgtat cgatccttat gagagcaccg ttgaagaact
      601 tcggtgacgg tgtctccgct gggatcataa ttctcgacaa tcttataaaa ggattgaacc
      661 ttcacctggt tggaatcaac cagcgttccg tcaaaatcga aggtgacgca agcaatcatt
      721 gcgcatgagg atcgggcaat ggcacttcgg gagttttctc cgatgctgcc tgctgggcga
      781 tttgaaaccg tccccaatga tcaaccattg ttttccgctc agatcgttgc agcttattga
      841 ttcgtttgtc ggcatagaaa gaatcgttgt tatagtgggt ggtggacact tcctcaaaga
      901 caaccccggt gtctgtccaa aaggaatgcc atactccagg taggataaga atggtttcac
      961 cgggatgcaa aatgcgggga tatccgtcga tattcacgtg gaggatacca aacagtattt
     1021 gaaatgtttc ctccttcttt gcatgataat gtgaggggtg tcgctgccca ggcaattgaa
     1081 tgacgagttt tttgcaatat tcccgattaa tgcattcgat aaggaccgcg ccggtttgtc
     1141 ggaaattttc aaggccgtag tggtgcgaat attcgacttt aaattcactg cctaattgaa
     1201 tgttggcttc gttgagtaag gctttgactt cgtgaatcgc ctgtttaaga gtcacgagac
     1261 cttgattgag ggtgatctcg acagcgtccc gcatgaccgg ttggtccggc gttatgtcct
     1321 ggacggccgt atagccttct ttccatgctc cgctttccat ctgcccttcg acgtagggca
     1381 tggcaaaata cacaaggtcc cgtgtgagtt cccggccttt tttaatggat cgtttggcaa
     1441 aaacccctcg acgaaggccg tccaaggagg actgttccac ttcggaggcc ggtggacgag
     1501 tttttgggcc acacaattct tttgcccgat gataggcttc cagccaggtg tccacttgtt
     1561 gtgaggtgga cgagtaggca ttcaatttga tggattcggt ttccagtcca atgtgccgtt
     1621 caaaaagtct ggctccaaga gcgaccgcaa tttgaacagg gacggtatca ccggggtttt
     1681 catgcgtgga ccagccgata ggaatattgg gataccggtt gcgcaatatc tgtatttgat
     1741 tcaagttcat taacgggtca ggagttggat acaccgacac gcagtgcatt aatgcgaatt
     1801 gcacggcccg atgttggaag aaactcacca cattatcgat gtcctccagt gtcagtccac
     1861 cggttgagca caccaccggc ggcccggcgc cggcaatttc ctcaagaagc ggccaatcct
     1921 tggctgaaca actggcgacc ttcaacacat taaaacccat gtcgacggca atattgacag
     1981 actcttcatc gaagggggtg cacatggcta gcagcccttg ggcccatact tcattgagca
     2041 atgtttggaa ttggatctgg tccaatcggg tggattgaaa tcgttggatg tatttgagct
     2101 cgctgtttga ctgatgatct gtatgaatga aggtgtcgag ttggcggaat tggaatttaa
     2161 tcgctgcccg gacttggtgc cgtttgatca cctcagccgt tgattggatg attcccaggc
     2221 cgtgttccac acttccctgg tggttattgg ctaggtccaa tatgaataaa tcatggaaat
     2281 tgaaatcttg gtctatcatg cttttcccct tcctctggca gatctacagc aattctagtg
     2341 cctctggtgt ataccctttc cggtaatcga tgcgcacagt gcgtttcttg gctgacgaga
     2401 gatgcgcggc ggcgaccacc agcatggttt ccaggcctcg ctccaatgag aggacggaat
     2461 cttttgtctt gcctgtcatc acgccctcga tgtgttgtaa ttcccagatg aaatcatccg
     2521 gtcgcgtttt ttgaatggta cggaattggg tgtcatcgcc accggttttc caatcgactc
     2581 tgtcgacccc tggttggtgc ccgatggtga gttccgcgaa accgtcattc ccttgaatcc
     2641 ttgcccactt tctggaaggg gaggtaatga cgtcttgcac gacccttccg agtaaacccg
     2701 cttcagtcct caggctaagc aggcagagtt tgtcgtactc caccatatct gtctggacat
     2761 aatccagtgt cgcggatact tcggtaatgc ggccttttcc caggccccca ctcagaaatt
     2821 gccataggtt aattgcatgg gaatgttccc cgctggcacc tccaccccgt ttccaaaacc
     2881 ccaaataact gtcctgtgga ccctgtaacc atggatgagc cgcgaagatg ccgccccaat
     2941 gttcacgaaa ttccacgtcg atggtttcaa tggtggataa ttggcgaccg gcttggagtg
     3001 tttcctgcgc ggcttttccc acaacgtggt catatcccac aaatgcgaat gtgccagatt
     3061 cgaccaccat cttctgtaat gcctgggcct gttccaagtt cggggggcag agaggttttt
     3121 ccacaagaat ggctttgggt ttttcctcaa gagcaccaag tgccagggag aggtgggagt
     3181 ctggaggagt cccaacaacc acgagatcaa atccccctct cggggcgtcc ttgaccaggg
     3241 aaagcttgat ggcctcgtcc cattttccat aacgagtcgg ataaatggtc tgtttggttc
     3301 gatccaatgc cgcttggtcc acatcgcata tcatgacgtt ccaacccagg gaacgagatg
     3361 catgagccaa atggttgccg atagacccgg ctccaaaaat ttttacgtta tacatgtatc
     3421 ctccttaggg tgttcacgaa atttggtcta tggatgccca tgcatgctca gcatgattgc
     3481 ggttataggc attcttcatg atggagtagt tagttgggaa atatttcaaa aatattgtcc
     3541 tgcaggggcc tgacttgtgt gtggtgaaat cttcctaacg cgtgttgcaa gcgctcaatg
     3601 cgttcttcga tatgccccag ggtgatatca gggaaaatat ggttcaaata attgtaccgt
     3661 tgtgatcggt tttgatcccc catgcgaaat tgagcgttga atcggcgtcg taattttctg
     3721 aggagggcct gagattttgc ggcgaatacc gttccccatt ttggggtcga gggtgaacag
     3781 gtggcgtcaa atgtttctag agtactcacg atgttttctg aggcaaaagg cccttggagg
     3841 gccgcaagat attgttcaat taaccgggtt ttagcttcag agccattttc ctgaggagac
     3901 tgatcacctg aaagggttgc tttgatgagt tcaattaatt cccgggcgga tgttgcccgg
     3961 tggctcacgg cattcggaag ttgtttgtca aaacggtcgg agatgatcgg ctgataggaa
     4021 aaaacgggac gtttcaggac atacccttcc aagccggtgg tgcaactatt atgaatgatg
     4081 gcatcggctg ctaatagcca ggggatgaca tgtccctcta tatgtacctg gacgtttgta
     4141 tatccgtttg cagcctgcct ccaagtctcg tggttttcag atggatgggg acggattaag
     4201 atggtatgag cgggaaaggc tgcggctatt tggcctacca tttcctgaaa atactggaat
     4261 agcgccagac ggtgccgtgc cattccaagt tcaaattcgt ctacgtccga tggtgaaacc
     4321 gccgcccgtt caagcgcatc tttttccgaa cctttgttgg gcctaaaatg gttcaattta
     4381 ccaaaattgg tattgatcag gatgttcttc ccaaattttt tatggatggt ttcgacttgc
     4441 ggtgtccaat attggcgcat ttccggccgt agtaagtcta gccgggaatt tccggttata
     4501 tgcacaggca ttccattgta tgcgggctgt gatttccaga ttctggcatt ttctggtccc
     4561 catgctaaaa agagattcgg ccgggaaaat tctgtcgagc ccaatttttc tttcacatac
     4621 acttctggcg aacaatacac aatggcttcc tcgtccgttc ctaccagcga gtgccccaat
     4681 tgggccagaa gggagaagac gatggcattc cggttatgaa gattggtggg caggtagatg
     4741 gatttgggta aagcggccag attgagttga atttcgtgct tttcccctat aatgacggaa
     4801 aatccacgct ctgtggcgaa gcaggaaagc agaactttgg cgtcaaattc cctattaaag
     4861 atttcaacgg gaatggtcag acggcgtcgc ggttttgtca tttcaaaaat atccgaacac
     4921 gattcggagg gcgctttgct ccggctgtgt aatggtgact ttgttataga tgatcaaaga
     4981 ttccaagagt ggctttgact tttcgggcga ccgtttccgt ttcctttaat tgtgggcgtg
     5041 ttccgtttgt aatgggttct ccgaacggtc tggcaatccc attcttgata agggaatcat
     5101 gcagcatggt gagatttcga cggggttgca ccaaacccgc acgaatcaat cgagcgcaaa
     5161 gccgttccaa tccctgctgt ggacgagccg ttccccgttt attcaagggc tgggattggg
     5221 ctcgttgaat aatcttttca ctgagccgta ctttccacgt tggggaattt tcgggaagtg
     5281 ggacaatata aaccggcttg cccagagaag ccgcctcagc caacatggat tcgctttcac
     5341 ctgtgacgat aatgacatcg gcatcagcca aataggcgaa ataagggtta tcagactggt
     5401 ttggcttcca tgtatgcaca aaatgatcag ggagcaatgt tgactcaaga gcgtgaatca
     5461 ggtctttgcc cgttctggga gacgtgacgg caaaaatttt cccatggcat ttttgcgcaa
     5521 ggtgtttgag ttggcctcca attgtgcgag ccatatcggg ggtaaatttg aaccgtgccg
     5581 tggcacctcc cacgacgaag acgatgcgag gatggggagc gttgtcgaaa agatttggcc
     5641 atcgctgctt caccctagcc aagctgtcct gcgttaattc gttcaagggg gctaccgtct
     5701 cgatgcggtg tggatgtgga gggaagccgt aataggacgg ggtgatgacc acatcaaata
     5761 agtcagccga gcatccgcct tttcgtccca attgaatgat acgtgttttc ccctggcttt
     5821 gctcacgaat ccatctggca attggtgccg gtcgccatcc ggtagaaatc acgaggtctg
     5881 gccatggggg gcagagaggg gaagagtgaa ccctatctac gccgatcaaa tttggaggga
     5941 ataatcccca cacgagtttt tggattttat gccatccata gaatcgcaac tgtttaattt
     6001 cataaggcca ccccaacgac ttcgcgagca cttctgattg agtggtatgt ccaactttgc
     6061 cgtctgagag aatccagatt cgtgggggag aaccgatcca atggcctcca caacggataa
     6121 atatttgttg aaatccctta ggtgtttttg cgtgaaacgc aagcttccag tgaatagccg
     6181 gatagcgggc gccaattgat tcaaggtgaa tcatccaccg gttaatattt tgtttcaagc
     6241 aggcgtgggg attttcagca ccataggatt ggtcggggtt ggtgatttct atcgccatat
     6301 agaccatgcc atgtgcacgg ttaaacattt cttcgataac ccatgggata tcttcttctg
     6361 ggacgagatg aagccctccg ggtgacagga ccaggtcgaa ttgtcgggaa ggcaggtggc
     6421 cagagtcctc ctgacaaaga tccattgtgg tcatggttaa tgaggaaagg tctgtagccc
     6481 gaaggtgttc gggaacattt tcgaacagtt gcccaaaatg acaatacaga agggacttgg
     6541 cctggagggt gcgtactgct tcttgaagtt cgttaatggt ctcgacatcc tggaaaggag
     6601 atagtttcgt cggggaaata ttccggagat ctctcgtctc gttagccaat tctgtaaata
     6661 gggaggaggg atgtttccgc gaaaatacaa ggaatccttt ttgattggca ctgtgctcca
     6721 aactaaacca aacatcgccc tccaggcttt tttggtatac aaattcctga ggaaagggct
     6781 gccaaggttg agtatggaga gccgtataat ggaggacttt agattgtcct gcctgatatt
     6841 cttcatcacg ggcattccat tcgggttcta atggacccca gaggttgggt acttccaagg
     6901 cgcgttgcac cagtgcgttt tttttcttga attttgccgc ctcaagggac cagatggcgg
     6961 ccattttttt gcaatccatg accatcaccg aagtatcatt ccgtgcaatg gagagaaagc
     7021 catgatcgtt caaatcgtgg tcgaataata atccaggatc cgccaggtag atttgatcga
     7081 catcgttgta aatcgctcgc cctgcctggc ccgcaaatgc cgggatcgca aaacgataat
     7141 ttgtaaaccc cgtgagccat ttgcgccggt caaatccctt taattccttc atgagataaa
     7201 tttcgtatac tcgacctgga tcccgaacct gttcaatgga ccagatgagt atgcgttcgg
     7261 ctcggccttg ggctgattcg cttccaacga aaattcgaac gggagctttt ccagaggaag
     7321 ctgtgccttc tctgacctcc aagatgattt tttcgggtat taccctgatg ccgttgggtc
     7381 tagagcggtg acgtgacatg ggacatttgg ggaatgatca ggatcgttgt ttcgacaggt
     7441 aagcgaaacg ctagcatttt ttaattgagg gttgcatctg gttgtaagtg agcagggttg
     7501 ctatgggttt gagttccatg aggcaaatca gcaaatgccg aaataatttt ttcaatttgg
     7561 ttatgtgtat gagcggcgct cacactgcag cgcaagaggc attcgccatt tggagtggca
     7621 ggaggaatca tgaggttgac ataaatcccg ttttccaaaa gttgttgcca catggcgaga
     7681 gccaattctt tgttggggac cattgcggca ataataggtc ctacctccgc cccaaggatg
     7741 tacccactct tcgttaaccc ctcatacaaa tgccgggaat tggtccacag atgctgacgg
     7801 agttctggat gagcctggat gatgcgaatg gtcgctcgtg tcgaagcaac aactgagggg
     7861 catggagaag cggtaaaaat atagggtcga ctggtgtacc ggattaattc gaactcggga
     7921 tgaaagctta cgccgaaccc ccccgtcgag cccaggcttt tgctgaaggt cccgacaata
     7981 caatcgacgt cctgttcgac tcctgtttct tcggctaagc cacggccatg ttgtccgagt
     8041 acccccaacg aatgcgcttc atcaacgatc agagtggccc catattttct cttcacttcg
     8101 acgatttcgg ccaacggggc tcgatctccc agcatgctat aaataccctc aacgacaatt
     8161 aaggttcgat aacttcggtc gcccaatcga cggagccgtt tttccaaatc tgccacgtcg
     8221 ttatgcttaa accggataat ctccgcgcct ccaagtcggc atccatcata tatgctggca
     8281 tgggagtccc catctatcaa aatagtgtcc cctgggcctg caagagcaga aagcactcca
     8341 agattggcct ggtagcctgt ggaaaagacg atcgcatagg gcgcaccata gaaatccgcg
     8401 atggcgtttt ccagcgcctt gtggctcgca aaagatccat tggccatcct cgatccggtg
     8461 gtgcctgttc cttcctgggc aagcgcctgt tggccggcta gaatggagtc aggatgaaag
     8521 gtcaacccta agtaattatt ggtaccgacc aggatggtct tgcgaccgtt gatgatggct
     8581 tctgtcggag aaatgatctt ttcgacggtg acgtgaaacg ggttgacccc acttttcagt
     8641 agctcgtttc tggcttcttg aatgggaaga aatttgtcaa atagtgacat gattaccctt
     8701 tcagcaactt ctcaatctct tcaccaaggt ccttgactgt tcggatattg ggaagattgt
     8761 tcagagggat ggaaatatca aaatgatctt
//
LOCUS       DATIAL010000441         3651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1262852, whole genome shotgun sequence.
ACCESSION   DATIAL010000441 DATIAL010000000
VERSION     DATIAL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262852"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..620)
                     /locus_tag="VLA60_13660"
     CDS             complement(312..620)
                     /locus_tag="VLA60_13660"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HSF10454.1"
                     /translation="MVRQFFLGFVKIHILHHAAEEPVCGVDLAQELASHGYQLSPGTL
                     YPTLHGLEAAGYLRCQLELQSGRRRRTYTITRSGRQALAQARQQIRELTEEVMNRKTI
                     "
     gene            1588..2085
                     /locus_tag="VLA60_13665"
     CDS             1588..2085
                     /locus_tag="VLA60_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HSF10455.1"
                     /translation="MNCLLLRHGIAVSSEEWNGSERARPLTKEGIHKTEQVAAGLKRI
                     GMKPTHLLCSPLTRTHQTAEIAKEALQITTAIQLCPELVYDQSPMLLFSILQGLPQDA
                     VVMCVGHEPHLGQTAALMIFGKNSSGLSVKKAGGCLISFDGNVGVGRGNLEWWMAPAQ
                     LRALR"
     gene            complement(2200..2592)
                     /locus_tag="VLA60_13670"
     CDS             complement(2200..2592)
                     /locus_tag="VLA60_13670"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HSF10456.1"
                     /translation="MAESYQMGALQVSTVGLEEEGYQMYLGQKCRLSGVLKVQGMARI
                     DGFVEGEIYGSDLIQVGKDGKLEATVKAKDIVAQGPLRGDFVALQKIQLLAPATLEGK
                     IDAPVFLLEEGVFVNGLVKMGMARPAVS"
     gene            complement(2934..>3651)
                     /locus_tag="VLA60_13675"
     CDS             complement(2934..>3651)
                     /locus_tag="VLA60_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HSF10457.1"
                     /translation="NFMTLHMGLPSSSSHALIGAMVGAGFIHGGVEVLQGAGLLKVME
                     AMVSSPLLGFLAAFVLMVGLSWAFFRTSRGRAMRLFRRLQLVSAGFMALSHGANDAQK
                     AMGIITLALLSAGHIPSAEVPKWVVISCALSMGLGTALGGWKIIRTLGMRIAKLDPVH
                     GFAAETGAGAVLMVTAHIGLPVSTTHTITSSVMGVGAVNRLSAVRWGVTRRIAYAWIF
                     TLPGSAFLAVAFYILLSAFF"
BASE COUNT         1007 a          942 c          776 g          926 t
ORIGIN      
        1 aatgattcaa ggcatattca atggcttgca gcaaggtcag ttgggtggac aactgttcaa
       61 gcggttgagc atgacaagct tctataacca catgaagact caaaaagatt gccaggagag
      121 ttggcaacag attcattgag gtgtccttct tatatattat tatggtgacc attgaccatt
      181 tacgtgttga gaaatatcac aggatggggc tagatgaatt tggaacacat ttttttcttc
      241 tcatccttcc tgtacaaccc taattcagat caccgtattg attagtaagt gtttccagca
      301 atctcctttc atcaaatcgt ttttcgattc atgacctctt ctgttagttc tcgaatttgc
      361 tgcctggctt gagctaaggc ctgtcggccc gatctggtga tggtgtaggt tcttcggcgg
      421 cgtcccgatt gaagttccag ttgacatcgg agatatcctg ccgcttccag tccgtgcaat
      481 gttggataaa gggtgccggg actcagctgg taaccatggc tagccaattc ctgagccaaa
      541 tcaaccccac agacgggctc ctcagcagca tggtgcaaaa tatggatttt gacgaaaccc
      601 agaaaaaatt ggcgtaccat acgccgagta agctcatgat tactggttgg cataaagact
      661 gccactaggg aaatagaatt ctgttatcgt ttatcgatat cgtttttcga tatcgttttt
      721 cgatactatt agcccaaact tccaaattgt caagatagtg attacacgtc gcatgcccaa
      781 gagtgagaga ccacaaacga aaccagatta tctccccatt agaaattcat tcctatagca
      841 accaaccccg atgcataggg tgcttgtatt ttggggctat ggcttgtttg ttttcttcat
      901 ctgattatga tgctctctta tcatgagaga tttatttcac aagcctgtct ctttggtgcc
      961 caggcccccc ttcagcatct ttgccaatac gtatgctgtg ttggcttata tcagcctatc
     1021 ctccatggat acttaggttg gccttgcaca gacaaacaat ttgattgatc cgcgtttaaa
     1081 aacgcccgtg ggttccctaa taagatatct tcactttgcc gggttcatga cagactgaaa
     1141 tcggcgcgaa acctcagaca ggaaaccagc gttgtcctgt ctaagctatt tatcccctac
     1201 tcgcccctga ttctttttta tcgtctttac cgcgccacaa atcgacgtct taagcgaata
     1261 tcgcgatgta tttgtagcgt tatcaaagat taccttcgcc ctcgtcggag cagtgataaa
     1321 tggggcaaaa ccctgggctt gtgggatttc cacccaaaat gattggcaga gaaatgccat
     1381 gacgttgctt ttcgcttttc tattgttgcc ttcgttacac gatttcaatt acaagtgaac
     1441 actttctgtt tattcctcat tggggttcct ggtcctcagt ttcaacttgg caatttttaa
     1501 cttttgcagt gcctgtagag ttgtcatttt tgatggatga taaccggcat ggtcgctctt
     1561 aatggggcaa cataaggaga tgttcatatg aattgtcttt tattgcgcca tggcattgct
     1621 gtttcttcgg aggaatggaa tggttcagaa cgtgcacgcc ccctcacgaa agaaggcatc
     1681 cataaaaccg agcaggtcgc ggcaggactg aaacgaatcg gaatgaagcc cacacacctg
     1741 ctgtgtagtc ctttaacccg tacacaccaa actgcggaaa ttgccaagga agccctgcag
     1801 atcacaacag cgattcagct ctgtcctgag ctggtgtatg accagtctcc catgctgcta
     1861 ttttccatat tgcagggttt acctcaagat gcggttgtca tgtgcgtcgg gcatgaacct
     1921 catcttggcc aaacagccgc attgatgatt tttggcaaga acagctcagg cctttcggtc
     1981 aaaaaggctg gaggctgtct gatttctttt gatggaaacg tcggagtagg gcggggaaat
     2041 ttagaatggt ggatggcccc tgcgcagctc agagcgctac gctaagggcc ttgtgcatga
     2101 cacaacattc accacttgtc aaccttatga aacatagaac aattcgcttt cgatgccatg
     2161 actgttactc ggtgcattgg ctaaatgtgt acccttggtt tatgagaccg cagggcgcgc
     2221 catacccatt ttcaccagtc cattgacgaa gaccccttct tccagcagaa aaaccggtgc
     2281 atcgattttc ccttccaatg tcgcaggggc cagcaactgg atcttctgca gagcgacaaa
     2341 gtccccacga agaggaccct gtgcgacaat atccttagcc ttcactgtgg cttcaagttt
     2401 tccatctttt cccacttgaa tcagatcgct accataaatc tccccttcaa caaacccatc
     2461 gattcgcgcc atcccctgaa ctttcaatac accgctcaat cggcacttct ggcctaaata
     2521 catttgatac ccttcctctt caagcccaac tgttgacact tgcaatgcgc ccatttgata
     2581 ggattctgcc ataactcctc cttatggtta caattccacg gattgggtga aaggaaaccc
     2641 gtgactccat ctttgaacac gtcagactaa agtgaacgat acgaaaggaa ccatccccaa
     2701 ttggagggac cgtagagcca acttccagac aaccaatttc ttaagatgaa atctacgtga
     2761 ctgtatccac aaaaccgggc cggtcaactg ttctgcaaaa gatgaacaaa cagaggagag
     2821 aaaaaacgga gaaatgcctt cacgtcaaga gtcaaggaac agaaaacctg aacctatcaa
     2881 ttcagcaaaa acagaatctt gagcatcaca gaaactgaaa taacaacgaa caactaaaag
     2941 aatgcggaca gcaggatgta aaatgcaaca gccaagaacg ccgaaccggg aagtgtaaag
     3001 atccacgcat aagcaattcg cctggtgacc ccccaacgaa ccgccgacaa ccgattcacc
     3061 gctcccacgc ccataaccga agaggtaatc gtgtgagtcg tactgaccgg caatccaata
     3121 tgagccgtaa ccatcaacac cgcacctgcc ccggtttcgg cggcgaaccc atgcacagga
     3181 tccaacttgg cgattcgcat ccccagagtt cgaatgatct tccaccctcc gagggccgtc
     3241 cccaagccca tagaaagtgc acaggaaata accacccatt tgggcacttc cgcagaagga
     3301 atatgtccgg cagaaagcag agccaaggta ataattccca ttgctttttg tgcatcatta
     3361 gctccatgac tcaacgccat aaaccccgct gacaccaatt gaagacgtcg aaacaatctc
     3421 atagcccttc ctcgcgaagt ccgaaaaaaa gcccagctca accccaccat gagtacaaaa
     3481 gccgcaagga atcccaataa aggagaagag accatcgctt ccatgacctt taacaatcca
     3541 gcaccctgca acacctcgac accaccatga ataaaacctg caccgaccat ggctccaatc
     3601 aaggcatggg aggaactgga aggcaacccc atatgtaagg tcataaaatt c
//
LOCUS       DATIAL010000442         9816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_80486, whole genome shotgun sequence.
ACCESSION   DATIAL010000442 DATIAL010000000
VERSION     DATIAL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80486"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VLA60_13680"
     CDS             <1..453
                     /locus_tag="VLA60_13680"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HSF10458.1"
                     /translation="GLSKLAFRQGVPTLGICGGMQSMNVALGGTLVQDIPSQLSTKIE
                     HRPTKSATKTAHLVNIEPNSLLQRIAKQLSIAVNSSHHQSIKSVPSSFQVTGIAPDGV
                     IEAIEASRHPFWLGVQWHPEFLYRQDAIQKRLFSALIQSARQFADSRT"
     gene            complement(530..937)
                     /locus_tag="VLA60_13685"
     CDS             complement(530..937)
                     /locus_tag="VLA60_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10459.1"
                     /translation="MDSLPELVGTVHIILGPYRGNPIALYMCQDESTCRISPRIYPWN
                     QTVGTGETPNKAAADFEARWKETDLADDMYTGPHWEGGIKPEKPKPVPAPKPATPPAA
                     KTEAAQETKTPAPKSPALPDPPQPAPEAEDAAP"
     gene            complement(1092..1571)
                     /locus_tag="VLA60_13690"
     CDS             complement(1092..1571)
                     /locus_tag="VLA60_13690"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSF10460.1"
                     /translation="MLGWTPESIKQWVRWMQFLDRWGYITACLSFLLVGMLVFGYSWV
                     AYAQSVQTEFLPATITLLNDLLFVIILLELFRTVLGFLQNDRIRLEPFLHVGIIASVR
                     RILTAGAEFSHQESVPEDMFRHYLMDMSLHVIVILVLMIALYLHRKSELPGEPLLNK"
     gene            complement(1587..1988)
                     /locus_tag="VLA60_13695"
     CDS             complement(1587..1988)
                     /locus_tag="VLA60_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10461.1"
                     /translation="MALKKGDIIDERKRYPDSCGLVLKVMSGGEGFEKIRCCGHDLTL
                     EDVVPSFNQERGRRAGALAVGMPLDEKMVATDSCGLRLMVMAGGEGFQEVICCGHSFG
                     AKAIQSLEFGQMRGEDTNREQSPNTPHQGTA"
     gene            complement(2198..3280)
                     /gene="nagZ"
                     /locus_tag="VLA60_13700"
     CDS             complement(2198..3280)
                     /gene="nagZ"
                     /locus_tag="VLA60_13700"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HSF10462.1"
                     /translation="MSIAELVGQLCMIGFEGTEVTPDLRAWMQEFRPGGIILFSRNLV
                     DPVQIAHLTNDLQALAGDIPLLIGIDQEGGRVSRLPSGFTIFPPATTVAHSGSTELAY
                     QAAAVTAKELRAVGINMNMAPVLDIHTNPSNPIIGDRAFGTEPEQVCLMGAATLAGLH
                     DHRVLACGKHFPGHGDTSTDSHVELPVVHATRERLESVELKPFQYAIAHGLQAIMTAH
                     VHYPALDPINPATLSSMILTDILRQQMGFSGVILSDDLEMGAIVNHSTVGEAAVHSLQ
                     AGVDMLLVCKTRGLATDTIKAVSRAVESHVLDQSRLETSLARIAFVKQQFVSPYHPID
                     IPSIPNSVGIFAHRHVLAQIQEQFRT"
     gene            complement(3296..4801)
                     /locus_tag="VLA60_13705"
     CDS             complement(3296..4801)
                     /locus_tag="VLA60_13705"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HSF10463.1"
                     /translation="MNILVKSGEATATTTDVLVCLEYEQDKTWSKAVRPVDQKLGGQL
                     SALRKGGEFSGKPNNTALLHIDGKLPAKRVLLVGLGTRETVTLERIRQAMGTAAKRAR
                     TAKAKGIVCVMPDVPKGTGPMDDVAQAMVEGVVLGEYRFNEYRTDRTDDNHSLQSCTL
                     LTTSNAHLNEAKDGAKRGQILGEATCFVRDLCNHPANVMTPSRVVTEAKKIAREAKVR
                     LKVLDRKQQEKLGMGGLLGVSRGSIEPPQFIILEYMGGPRTQKPIVFVGKTVTFDSGG
                     ISLKPSENMEQMKADMTGGAEVLATIRAASRLKLPVNAIGLLPVTENMPGGRATKPGD
                     ILTMLSGKTVEVQNTDAEGRLILADGLAYASRFKPACVIDIATLTGAAAVALGQFAIG
                     MLGNDDPLKADIKKAGNQAGERVWEMPLWDEYFEQLKSDVADMRNIGGRGGGMITAAM
                     FLSKFVGDHPWVHLDIASTDWGTTERPYIPKGPTGIGTRLLIQYLLNHVNR"
     gene            complement(4862..5515)
                     /gene="queE"
                     /locus_tag="VLA60_13710"
     CDS             complement(4862..5515)
                     /gene="queE"
                     /locus_tag="VLA60_13710"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HSF10464.1"
                     /translation="MTTLPSLKITEIFHSLQGESTRVGQPCVFVRLTGCPLRCTWCDT
                     EYAFYGGHTMPIETILTQVQSYGCPLVEVTGGEPLHQPGSLVLLTQLCEAGYQVMLET
                     SGAFDIANVDERVSIILDVKCPGSGMTDRMRWDTLRHLSGKDEVKFVLKDRVDYEWAR
                     DIVKRYSLGDRCAVHLSPVFGVLHLQSLAEWILEDRLPVRFQLQLHKVIWDPQTRGV"
     gene            complement(5761..6456)
                     /locus_tag="VLA60_13715"
     CDS             complement(5761..6456)
                     /locus_tag="VLA60_13715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF10465.1"
                     /translation="MTRSTSSPAPLTHSQHWIGAFFDVDNTLIPGASIEIGFFRYLWQ
                     DGVMGWPEVWHSLEYAIRQMPPISFNPLRRRKLYLMGHDVSLIEQMASEYVELYVVPR
                     VSARALNRLSRHRDAGHVVAFVSGSPEFLVKPLAEALGVSLVFGARPESQEGLYTGKV
                     FAPLPYGEGKGRYVERLATRFNLDLTRSYAYGDSPGDFHALQLVGHPFVVNPIRGMAR
                     IARERGWPITQWA"
     gene            6568..6651
                     /locus_tag="VLA60_13720"
     tRNA            6568..6651
                     /locus_tag="VLA60_13720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6601..6603,aa:Leu,seq:gag)
     gene            7116..7739
                     /locus_tag="VLA60_13725"
     CDS             7116..7739
                     /locus_tag="VLA60_13725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF10466.1"
                     /translation="MKRGWYSRRVFPRLVHWSMGQAGFAPLRQALIAKASGLVLEIGF
                     GSGANFSYYPSQIHSLTAIDPNPGMIPLARSLQAETVIPVHLALALAEALPFPSASFD
                     TVVSTMTLCSVPQFSKALQELFRVVRPGGRLLFLEHGQSPDRSVRLWQDRLTPAWKRL
                     GDGCHLNRPMVQMIQAQGWTVSALENFYLPGVPKPFAYFSQGMAVKA"
     gene            7839..>9816
                     /locus_tag="VLA60_13730"
     CDS             7839..>9816
                     /locus_tag="VLA60_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10467.1"
                     /translation="MAKFLLMVNRGVPAYLTVWAAMLGVSLSLGFLIDSLSYAEVAGA
                     SQGASSPVQDSAQAHLQLGIDFFLTDDLDVAIDEFRETARQRPGYADAYHNLGVALAK
                     TGDLTGAIAAWSQAERLDFHTASLRYHLSALVSYNYGISLVRDGRLAQALAQWRAALR
                     LQPDFPEAHYALGLGYLAAGDPVQAVAHFQATLHDESNWAHAYEALGLAYFASHENIL
                     AEQAWRKALALEPDLPKVHANLGLLRLQEGNYQEAIEHSRQALTLQPDLVAGHYNVGV
                     ALFAKGDESASVPSLERAISLDSRLTSARLLLGVVWSRQGNWAQAASLWREALRRDPF
                     AKDGVWLHYNLGVAMAAMGLIDEAAVEFDVVTDQRPEWAPGWSQLGSALLATRQWEKA
                     VVALESAARLQPAWAHLHFAIGKAWAEAGNLSQAVAAFQRAVEVEPQLVDAWFHLGVV
                     LRAQNRIGEAIEPLRLAAEGGSGEAQSLLASMYANGSGVDRNIPLAMLWWFRSSRATM
                     ADSLTQTAREQLSQFRRGLHRHLFSPSDRQQVLIGFSLIREDLHHVSPPHRVSTQVAN
                     DEGTWDHLTPSEPVVAWVIDQALAGDQSAQYTLHDWYVNGERGRLVPADPQIRNYFLQ
                     TAKEGNPFSCQVVRAVAPESAERFSADWQLAANGC"
BASE COUNT         2221 a         2540 c         2667 g         2388 t
ORIGIN      
        1 ggactatcca agttggcctt tcgtcagggg gtgccgacgc tggggatctg cggagggatg
       61 caatccatga acgtggcctt gggaggaacc ctggtgcagg atattccctc tcaattgtcg
      121 acgaaaattg aacatcgacc caccaaatcg gcaacgaaaa cagcccatct ggttaacatt
      181 gaacccaaca gcctactgca gcgcatcgcc aagcaattga gtattgcggt gaatagctcc
      241 caccatcaat cgatcaaatc tgttccgtcg tcctttcaag ttactgggat tgctccggat
      301 ggagtcattg aagcgattga agcctctcgt catccattct ggcttggtgt ccaatggcat
      361 ccggagtttt tataccggca ggatgcgatt cagaaacgcc tattttcggc gttgatacaa
      421 tccgccagac agtttgccga cagccgaacc tagcctgttc aggacatttt ggcctggttt
      481 ccacaaaatc catacgaaag agaactctcg ataaggggac tgggaattct tacggagccg
      541 cgtcttcggc ttcgggagct ggttgtggtg gatccggcag ggccggtgat ttgggtgcag
      601 gggttttggt ctcttgtgcg gcctctgttt tggctgcagg aggagtggca ggttttggag
      661 caggcacggg ttttggcttt tccggcttga tgccaccttc ccaatgaggg ccggtataca
      721 tatcgtcggc caagtccgtc tctttccacc tggcttcaaa gtctgcggcg gccttattgg
      781 gagtttctcc ggttccaaca gtttgattcc acggatagat acgaggagaa attcggcagg
      841 tagactcatc ctggcacatg tacagtgcaa tcggatttcc tcgataaggg cccagaataa
      901 tatggaccgt gccgactaat tcaggaagag aatccatgat accaacacaa cagattgtcg
      961 tctaaaaagg aggctatact gccacagccc ccggcggtat gcaatatgag ccgaagaagt
     1021 tcatcaggaa tttatgacaa tgattgaggg aagagaaatg agaaacagaa ttttcctgaa
     1081 attcggaagg gctatttgtt gagaagaggt tctcccggga gctcggattt tcggtgaaga
     1141 tataatgcga tcatgaggac caaaattacg atgacatgca aggacatatc catgaggtaa
     1201 tgacgaaaca tgtcttcagg gacagattcc tggtgcgaga attcagcgcc agcggtgaga
     1261 attcttcgga cagaagcgat gattcccaca tgcaggaagg gttcgagtcg aatacggtcg
     1321 ttttgtagga agcctaatac cgtgcgaaac aactccaaaa gaatgatgac gaacaacagg
     1381 tcattcagta aagtgatggt cgctggcaaa aactcggtct gtaccgattg ggcataggct
     1441 acccaactat aaccgaagac cagcataccc acgagaagaa aactcaagca ggcggtgatg
     1501 tagccccacc ggtcgaggaa ctgcatccat cggacccatt gtttaattga ttctggtgtc
     1561 caccctaaca tgttgaatct gctctcttac gcggttcctt gatgtggcgt gttgggggat
     1621 tgctctcggt tggtgtcctc tccccgcatc tgaccaaact ctaaactctg aatcgctttc
     1681 gccccaaacg aatggccaca acagatgact tcctgaaatc cctccccgcc cgccattacc
     1741 attaggcgaa gtccacagga atcagtcgca accattttct catcgagggg catgccgacg
     1801 gccaaggctc cggctcggcg gccacgctct tggttaaacg agggaaccac gtcttccagg
     1861 gtcaggtcat gtccgcaaca tcgaattttt tcaaatcctt cgccaccgct catcacctta
     1921 agtaccagtc cgcaagaatc cggataccgt ttgcgttcgt cgataatgtc cccttttttc
     1981 aatgccatag cgatgctcct tgctcccgaa atgaaacatg aaattctgtc atcagcaata
     2041 ggaatagatg aatggatgtg tcggtcaata cagcaaggtt gatagctaaa caaatctttg
     2101 tagggaatgt ccagaattgg gccaggccct tctttttcac acctaaaacg gtgagagtag
     2161 ccggcatccg tccgtcttgt ctttctgtgc attcaggtca ggtgcgaaac tgttcttgga
     2221 tctgtgctaa cacatggcga tgggcgaaaa tcccgacgga attcgggatg gagggaatgt
     2281 cgattgggtg atagggggag acaaattgct gtttgacgaa ggcgatacgg gccaaactcg
     2341 tttcaaggcg gctttgatcc aatacatgag attcaactgc acgactgacg gctttaatcg
     2401 tatcggtcgc aagcccacgg gttttgcaca ctaataacat atccactccg gcttgcaacg
     2461 agtgcacagc ggcctcgccg acagtcgaat ggttcacaat ggcgcccatt tccaggtcgt
     2521 cgctcaagat gacgccggaa aatcccatct gctgccgaag gatatctgtc aatatcatgg
     2581 aagagagggt tgcgggattt atgggatcca gggcgggata gtggacgtgg gccgtcatga
     2641 tggcttgtag gccatgcgca atggcgtatt gaaaaggttt gagttccacg ctctccagcc
     2701 gctcccgggt ggcgtggacc accggcagtt ccacatgaga atcggtactg gtgtcgccat
     2761 gaccaggaaa atgttttccg caggccaata cgcgatgatc gtgcaggccc gcgagagtag
     2821 ctgctcccat gaggcacacc tgttccggct cggtcccaaa cgcccgatct ccgataatag
     2881 gattggaggg attcgtatgg atgtccaaaa ccggggccat attcatatta atcccaacgg
     2941 cgcgtaactc cttggcggtc acggctgctg cctgataagc cagttccgtg gatcccgagt
     3001 gggcgacggt agttgcagga ggaaagatgg taaagcctga aggaaggcgg gagacgcgtc
     3061 cgccttcttg atcgatgccg atgagtagcg gaatgtcacc ggccagggct tggaggtcgt
     3121 ttgtgagatg tgcgatttgg acagggtcaa ccaaattgcg ggaaaaaaga atgatccctc
     3181 ctggtcggaa ttcctgcatc caggctcgaa ggtccggagt gacctccgtc ccttcgaacc
     3241 cgatcataca cagttgccct acgagttcag cgatagacat cgcgtgtgtt gccgcctagc
     3301 gattgacgtg attcagcaaa tattgaatga gcaaccgggt tccgatacct gtcggacctt
     3361 taggaatata cggccgttct gttgtacccc aatcggtact ggcaatgtcc aaatggaccc
     3421 agggatggtc cccaacgaat tttgataaaa acatggccgc cgtaatcatt ccgcctccgc
     3481 gcccaccgat attccgcatg tctgccacgt cactttttag ctgttcaaaa tattcatccc
     3541 acaggggcat ttcccatacc cgttcaccgg cctgattgcc ggcctttttg atgtcggctt
     3601 tcagggggtc gtcattaccc agcatgccaa tcgcgaattg tcccaacgcg acagcagctg
     3661 caccggtcag tgtggcgatg tcaattacgc aggcgggttt gaaccgagag gcataggcca
     3721 agccgtcagc cagaatcaac cgaccttccg catcggtatt ttggacttcc accgtttttc
     3781 cggagagcat ggtaaggata tcgccgggtt tagttgcccg tcctcccggc atgttttccg
     3841 tcacaggcaa gaggccgatc gcattgaccg gaagcttgag ccgggaggcg gctcgaatcg
     3901 tcgctaacac ttcggctcca ccggtcatgt cggccttcat ctgttccatg ttttccgatg
     3961 gttttaagga gatgccaccg ctatcaaatg ttaccgtttt gccgacaaaa acaatgggtt
     4021 tttgtgtgcg agggccgccc atatattcca gaatgatgaa ttgaggcggt tcgatgctgc
     4081 ctcgagagac ccccaataat ccgcccatgc cgagtttttc ctgctgtttg cggtccaaga
     4141 ctttgagccg gactttggcc tccctggcga tttttttggc ttcggtgacg acccgggaag
     4201 gcgtcatcac gttcgcgggg tggttgcaca aatcccggac gaaacaggtc gcttccccaa
     4261 ggatttggcc acgtttggca ccatctttgg cctcattcag gtgagcgttg gaggtggtga
     4321 gtagggtgca ggattgcaag gagtggttgt cgtccgtgcg gtccgtgcgg tactcattaa
     4381 accgatattc cccaagaact acaccttcca ccatggcttg agccacgtca tccatagggc
     4441 cggtgccctt tggcacgtcc ggcatcacgc aaacaatccc tttggctttg gcagtgcggg
     4501 cgcgcttggc agcggtcccc atcgcttgac gaattcgttc caaggtcacc gtctcccgtg
     4561 ttccaaggcc gacaagaagc acgcgtttgg cgggcaattt cccatcgata tggagtaagg
     4621 cggtgttatt cggcttgccg gaaaattctc cacctttgcg aagggcgctt aattgcccac
     4681 cgagtttctg atcgaccggc cggacggctt tgctccaggt tttgtcttgt tcatattcca
     4741 aacacaccaa cacatccgtt gtcgtagcgg tggcttcacc actcttcaca agaatattca
     4801 tacgcctcct caataaataa atcgccccta acggtaaaca gatccccttg ccgtggcgtt
     4861 gttacacgcc ccgggtttgg ggatcccaga tcactttatg caattggagt tgaaagcgca
     4921 caggcagtcg atcttccaga atccattcag ccagggattg aagatggagg actccaaaga
     4981 ctggactcaa atggacggca cagcggtctc ccaatgaata ccgcttcacg atatcgcgcg
     5041 cccattcgta atcgacccgg tccttcaaaa caaacttcac ttcgtctttt ccggacaaat
     5101 gcctaagggt atcccaccgc atccggtcgg tcattccact acccggacat ttcacatcca
     5161 gaataatcga aacccgctca tccacgttgg caatatcaaa cgccccgctg gtttccagca
     5221 tcacctgata tccagcctcg cataattgtg ttaacaatac cagagagcca ggttgatgca
     5281 atggttcacc gccggtcacc tcgacgagcg gacaaccata cgattgtacc tgcgtcagaa
     5341 ttgtctcaat cggcatggta tgtccaccat aaaaggcata ttccgtgtca caccaggtgc
     5401 aacgaagcgg acaacccgtt aaccggacaa acacgcacgg ttgtcccaca cgggtggact
     5461 ccccttgaag gctatgaaag atttcggtga tctttaagga aggcaaagtg gtcataaaca
     5521 ctctcaactc cagcacacaa cagatagcat tgtactcaaa aagtggggtg agggggaggg
     5581 gacagagaaa ggcgcgctag gtggctgtcg gagatgagta atcgtcacaa gactggatcg
     5641 ggttgagtga tattttcgat catatggggc gaatagaggg ggctattcga tgaaaaggac
     5701 cggagaagcg gtcaatttgg cgcaagcaag caggtggatt tcatttcggg tggccccctc
     5761 ctaagcccat tgtgtaattg gccaaccacg ttcgcgggca atacgagcca tgccgcgaat
     5821 ggggttcacg acaaagggat ggccaaccag ttggagtgcg tggaaatctc cagggctatc
     5881 gccataggca tagctacgcg tcaagtccag attgaaacga gtggccaggc gctccacata
     5941 ccggcctttt ccctcaccat agggcaacgg ggcaaacacc tttcccgtat agagtccttc
     6001 ctgggattcc ggtctcgcgc caaatactaa tgagactcca agtgcctcag ccagtggttt
     6061 caccaagaac tcaggtgatc cgctgacgaa ggccacgaca tgtcccgcat cccgatgacg
     6121 ggataaacga ttcaaagccc tcgctgagac ccgtggaaca acatataact ccacgtactc
     6181 gctggccatc tgttcaatga gggagacatc atgccccatc agatacagct tcctccgtcg
     6241 cagagggttg aacgaaatgg gtggcatttg ccgaatggca tattccaggc tgtgccatac
     6301 ctccggccaa cccatgacac cgtcttgcca gagataccgg aaaaatccga tttcaatcga
     6361 cgcacccgga atcaacgtgt tatccacatc gaaaaaggca ccgatccagt gttgagaatg
     6421 agtcaggggt gcgggcgaag aggtcgaacg ggtcatggtg agctataatc cgggcggtca
     6481 aattattaat gagaatggcc gggattctac cccgcgcccc acgcgttgac aaggacacca
     6541 agaggtttca taatctgcct acttcgtgcc gaagtggtgg aatggcagac acgcacgttt
     6601 gaggggcgtg tggggaaacc catgcgggtt caagtcccgc cttcggcacc aacaatccta
     6661 cctatctcta gatcgtctct gtcatgggtc ctacccttac aaaaaattgt gccgagattc
     6721 gaattgtatt tctgaattgg gagttccccc ttttataggg ggaccggaat gaagcgaagg
     6781 catgcgtgag ggcctttgtg agagcagaag ccagccttcc ggcacctgct cgttcaagtc
     6841 agatcttcag gctccaatcc tttcctccag attatcttat cgatggggct caggtatgcc
     6901 actgtcaccg catacattct cgaggtaaaa actttattaa cgaccttggc gttcgggtca
     6961 ttcattccta gaggaaccgg gggaaattga aagatttcgt cgatgggatt ccggctcatg
     7021 cgcttgcttg cggactactt tgggggataa acgttattct gatgcttagg aaagggcaaa
     7081 atagaagaca aattcaattg ccttgaaggt cattcatgaa acgcgggtgg tattcacgac
     7141 gagtgtttcc caggctggtc cattggagta tggggcaagc gggatttgcc cccttgcggc
     7201 aggcgttgat tgcaaaggct tcagggttgg ttttggagat cgggtttgga tccggcgcca
     7261 atttttctta ctatccttcc cagatccatt ctttgacggc gattgatccc aatcccggaa
     7321 tgatcccgtt ggcccgctcg cttcaggcgg agacggtgat tcccgttcat ctggccctgg
     7381 ccttggccga agcgctcccc tttccctctg ccagcttcga tacggttgtg agcacgatga
     7441 ccttatgcag tgttcctcaa ttttcgaagg ctctgcagga attgtttcgg gtcgtgagac
     7501 ctggaggacg gttgttgttt ttagagcatg ggcaaagtcc ggatcgttcg gttcgcctgt
     7561 ggcaggatcg tctcacgccg gcgtggaaac gccttggcga tggctgtcat ttaaaccgtc
     7621 caatggttca gatgattcag gcgcagggtt ggacagtttc cgcactcgaa aacttttatc
     7681 ttcccggcgt tccgaagcct ttcgcctatt tttctcaggg gatggccgtg aaggcctgat
     7741 cctccctgtg cgcccttcca ttttccttga tttcttaata cagtgaagtt ggtgttcatg
     7801 agggtgtcgt gtgtcttctt gaaccttccc caactctaat ggctaaattt ctactgatgg
     7861 taaatcgagg ggtacctgcc tacttgacgg tgtgggcggc gatgttggga gtttccctct
     7921 cgctgggatt tcttatcgat tccctctctt atgcagaggt ggctggtgcc tcacaagggg
     7981 cttcatctcc tgttcaggat tccgcccaag cgcatttaca gttagggatt gatttttttc
     8041 tcacggatga cctggatgtg gccattgatg agtttcggga gaccgctcgt cagcgtcccg
     8101 gttatgccga tgcctatcat aatcttgggg tggccctggc caaaacgggg gatttaaccg
     8161 gggccattgc ggcctggtcg caagcggaac ggttggattt tcacacagcc tccttgcggt
     8221 atcacctgtc cgccctggtt tcgtataact atggcatttc cctggtgcga gatggtcggc
     8281 ttgcgcaggc cctggcgcaa tggcgggcgg cccttcgcct tcaacctgat tttccagaag
     8341 cccattatgc attgggattg gggtatttag cggcaggcga tcctgtgcaa gctgtggccc
     8401 attttcaagc gacgcttcac gatgagtcta attgggcgca tgcctatgaa gctttgggat
     8461 tggcctattt tgcatcacat gagaatattc tggccgagca ggcatggagg aaagccctag
     8521 ctttggagcc cgatcttccc aaggtccatg ccaatttggg attacttcgt ttgcaggagg
     8581 ggaattatca agaggcgatt gaacattccc gacaggcgtt gacacttcaa cctgaccttg
     8641 tagccgggca ttataatgtc ggtgtggcat tgtttgcgaa aggagacgag tcagccagtg
     8701 ttccttcact ggaaagggcc atttctcttg attcccgatt aacttcagcg agattgctgc
     8761 ttggtgtcgt gtggagtcgt cagggaaatt gggcacaggc cgcctcgctc tggcgtgagg
     8821 cattacgccg ggatcccttt gccaaagatg gagtatggct ccattacaat ctgggagtcg
     8881 ccatggctgc catgggacta atcgacgagg cagcggttga atttgatgtg gtgactgacc
     8941 aacgtcctga atgggctccg gggtggtctc agttgggctc agcgcttctg gcgacccgac
     9001 aatgggaaaa ggccgtggtc gccctggaat ccgctgctcg attgcaaccg gcctgggcac
     9061 acctccattt tgccattggc aaagcctggg cggaagcggg gaacttgtct caggctgttg
     9121 ccgcctttca acgagcggtc gaggttgagc ctcaactggt cgatgcatgg tttcatttgg
     9181 gggtcgtgct gcgtgctcag aaccggattg gtgaagcgat cgagccactc cgtctggcgg
     9241 cggaaggcgg atcgggcgaa gcccaaagcc tgttggcttc gatgtatgcc aatggcagtg
     9301 gcgtggaccg caacatccct ttggcgatgt tatggtggtt tcggagtagt cgggccacga
     9361 tggccgattc gctcacgcag accgccagag aacagctttc acaattccgt cgcggcctac
     9421 atcgacatct cttttcgccc agtgaccggc agcaggtgtt aatcggcttt agtcttattc
     9481 gagaagatct tcaccacgtg tctcctcccc atcgcgtgtc cactcaggtg gccaatgatg
     9541 aagggacctg ggaccacctg actccgagtg aacccgttgt tgcatgggtg attgaccagg
     9601 ccttggccgg tgatcagtct gctcaatata ccctccatga ctggtatgtg aatggagagc
     9661 gtggtcggtt ggttccggca gatcctcaga tccgaaatta ttttctgcaa acggccaaag
     9721 aaggcaatcc cttttcctgt caggtcgttc gagccgttgc tcctgaatcg gctgaacgtt
     9781 tttcagcaga ctggcaattg gcggcaaatg gatgtc
//
LOCUS       DATIAL010000443         4147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_940697, whole genome shotgun sequence.
ACCESSION   DATIAL010000443 DATIAL010000000
VERSION     DATIAL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940697"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VLA60_13735"
     CDS             complement(<1..699)
                     /locus_tag="VLA60_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10468.1"
                     /translation="MSKLIEALNRLQSVRTEEGLPSSLSLLADSRPDKSESVQQHQPD
                     LKPSSKYNIHSEEIPRFNLALGEWLGILHHEYLGSFVREGGGAVKFAVFPSEDVLKTC
                     QQELEGLAKREGYVLAKVDARFTKAHMIERLFQKIAKQIDWDELAYRFLQRLLEEHGH
                     QIPSNRPEFSLRQVAMMNERKEPMLRRDIQTWMEKAIEGDSGLCREFRMAMIRLCMAQ
                     LDDGDSDRVMATAVK"
     gene            complement(817..1557)
                     /locus_tag="VLA60_13740"
     CDS             complement(817..1557)
                     /locus_tag="VLA60_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10469.1"
                     /translation="MSSSKRPPKEIHRHPRVVGTVPNWQREDTYPCAQSLNDTFWRWE
                     FLRRRADYRKDWEQHYLQTYDFDLSCAKDPQYPTRYRKKVFTPDHPAFKARMPNSLEK
                     YHLNGLPNPSIAKPWMLSFDSNYGRIYFGQGPDWLGGGEEVTLCLSEWRMAAVFDLKK
                     PLSGQIEKVKADLLEWQKHQLGRNLERRKCRDEWPMYLRVLDAVDLGVPFHEIGRTLF
                     HLHDDDICEARANQLYKRACQMSVHFPV"
     gene            2525..3151
                     /locus_tag="VLA60_13745"
     CDS             2525..3151
                     /locus_tag="VLA60_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSF10470.1"
                     /translation="MPDFKGLGAGAGPSPRARTSVILFHKPKGYLVTRKDERERKTVY
                     HILPPFLLQEGWVPIGRLDKDSRGLLLFTQDGTVVDRLTAPGGCEKTYEVEIRGRISD
                     DDLSLVLQGVSTSIGILTVHRVTKKREVGPKTQLEVVLREGKNRHLRRIFHALKDPKF
                     QTPLKVLDLKRVQIGPLPLDIPVGTWRFSTPDEETQLMAIFKIPIPRG"
     gene            complement(3171..>4147)
                     /locus_tag="VLA60_13750"
     CDS             complement(3171..>4147)
                     /locus_tag="VLA60_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HSF10471.1"
                     /translation="HRPLYDWLLRVCEVSCYPQQIEFARLNLGHTVMSKRKLHQLVEE
                     KHVNGWGDPRLPTLKGLRRRGYTPEAIRNFCERVGVAKRDSVIEMALLEHAIREDLNR
                     SAPRVMAVLHPLKVIIENYPDGQVEILEAINNPEDPTQGKREIPFSRELFIEQDDFRE
                     DPPKKFFRLSPGQEVRLRYAYIIRCVGVEKDPETGEVTALRCTFDSETKSGGSQSSRK
                     VKGTLHWVSAQHALPAEVRLYEALLTQPNPGSGGDAQDLHHLLNPHSLTVLSDCQVEP
                     GLRGAPSGSRFQFERQGYFCLDPDSTQDHLVFNRTVPLRDTWAKIDKK"
BASE COUNT         1113 a         1108 c          915 g         1011 t
ORIGIN      
        1 tttcaccgcc gtagccatga cacgatcgga atcaccgtca tccaactgag ccatgcatag
       61 ccggatcatt gccatgcgaa attcacggca cagacccgaa tcgccttcaa tcgctttttc
      121 catccatgtt tgaatatccc ggcggagcat cggctccttc cgttcattca tcatcgcaac
      181 ctgccgcaac gagaattccg gtcggtttga aggaatttgg tgcccatgtt cttcgagcaa
      241 cctttggaga aaccgatagg cgagttcatc ccaatctatt tgtttggcaa ttttctgaaa
      301 cagtcgctcg atcatgtggg cttttgtgaa tcgtgcgtcg acttttgcca aaacatatcc
      361 ttcacgtttg gccagtccct ctaattcctg ttggcaggtt tttaatacgt cttcactcgg
      421 aaacacggca aatttgaccg ctccaccccc ctcacggacg aaactcccaa gatattcatg
      481 gtgaagaatg cccagccatt cgccgagagc cagattaaat cgtggaatct cctcggagtg
      541 aatattgtac ttggacgatg gtttcaaatc cggctgatgt tgttgcacag attccgactt
      601 gtccggacgt gagtcagcga gcaaagaaag ggaggaagga agaccttctt cggttctcac
      661 ggactgtaaa cgatttaagg cctcaatcaa tttgctcatc gtacggttcc ttcccccatg
      721 tacattctgg aattatcaga cttctcccac tccacacctg attcaatgac catgcccaca
      781 cccgcactct gacgggctcc cgtaaccttg aacctcttac acaggaaaat gcacactcat
      841 ttgacaggca cgtttataca attgattggc tcgcgcttcg catatgtcat catcatgtag
      901 atgaaataac gttcgtccga tttcatgaaa cggcacgccg agatccacgg catccagcac
      961 gcggagatac attggccatt catcacgaca ttttcggcgt tcgagatttc gaccgagttg
     1021 atgtttttgc cattccagaa gatctgcctt cactttttca atttggcccg acaacggctt
     1081 tttcagatca aacacggccg ccatgcgcca ttccgacaaa cacaaggtga cctcttcacc
     1141 tccccccagc caatccggtc cctgtccgaa atagatccgg ccgtaattcg aatcaaatga
     1201 caacatccag ggtttggcaa tggatggatt cggaaggcca ttgagatggt atttttctaa
     1261 tgaattcggc attcgagctt taaatgccgg atgatccggg gtaaaaactt ttttgcgata
     1321 ccgagtggga tactgaggat ctttggcgca ggacagatca aaatcgtatg tttgcaagta
     1381 atgttgttcc caatccttcc ggtaatctgc gcgtcgacgg agaaactccc accgccaaaa
     1441 ggtatcgttt aaactctggg cgcagggata ggtatcctca cgttgccagt tgggcacggt
     1501 ccctaccact cgtggatggc ggtgtatttc cttgggcggt cgtttactgc tcgacatgtc
     1561 tccggacctc ctgttcggga gtattcactg ctagatcgcc ggatatgagt gacgtgccgg
     1621 gtccgaagaa agtcaaagac cgtcagtccc ggcttacgcg acattccagc aacctacctc
     1681 aaacccgcaa gcactcagga atactgataa cccggtcaaa aaaagaatgg tgtcggttct
     1741 taaactacaa tacgaacaaa agaggcaggt gtcgggagat gagctgcggg atctttactc
     1801 atgatgagga gaacaggttt tgcgtcttga ccaagacact ccatggaccc atccaaacca
     1861 ggagagcacc gtcacacaac aagctaaagc aaccatggcc aagacaaaac aaattttccc
     1921 taacatgtcg aagaccccca ggatagcggt tcttcattcc tgcctctact ctctgtatcg
     1981 gcagggtttt tacaggtctt tactcgatgc ctaaaaaaac ataccctttc tctaaagaaa
     2041 ataactccaa aaattctaca aaacaatggg caagactttc ttcggggtta tttgcatttt
     2101 ttttcttaaa aaacaatggc ttacataaaa atcgactgtt atatccagat acactaaaaa
     2161 taatccttct ttactctcta gcccattaac tgtattggga gacgcctcag gcgccgattt
     2221 caagtccgca acactcatca gagttcattt tcacggtcca gccaaggctt tgagcagatc
     2281 actatgccat tgaaccggac agtaaaaaaa acttataaaa tacagagccc ggttggttgg
     2341 cacaccccct catcgggacc agtgtgattg gatcagagta cccgaaccga ttagttgagt
     2401 catttctctc aaagaattaa ccctttcgca aagaaggagc caaaacttgt gagtgtcctg
     2461 cgacaagaac caccagagat ctcgaattcc tttatcactg tcgattcaac aaaaaaaaac
     2521 aaccatgcct gacttcaagg gattgggcgc tggagcagga ccttctccac gtgcgcgaac
     2581 aagcgtcatc ctgttccata agcccaaagg gtatctggtg actcgaaagg atgagaggga
     2641 gagaaaaaca gtctatcata tcctaccgcc tttcctattg caggaaggct gggtaccaat
     2701 cggccgattg gacaaagact cccgtggcct cctcctcttc actcaagatg gaacagtagt
     2761 tgaccggctc accgcgccag gcggatgtga aaaaacctat gaggttgaga tccggggaag
     2821 aatttcagac gatgacttat cccttgtgtt acagggagtt tccacttcca tcggaatatt
     2881 aacagtacac agggttacaa aaaaaagaga agtcggaccc aaaacacagc tagaggtcgt
     2941 cttacgggag gggaaaaacc ggcatctccg tcgaatcttt catgccctga aagatccaaa
     3001 atttcaaacg ccgctcaaag ttctcgatct caaacgcgta caaatcggcc ctttgccctt
     3061 ggatatcccc gtaggaacat ggagattttc aacacccgac gaggagacgc aattgatggc
     3121 tattttcaaa ataccaatac ctcggggttg aatctttggt gaaagcgctc tcacttctta
     3181 tcaattttcg cccatgtatc tcgaagaggt accgtccggt taaagaccaa atggtcctga
     3241 gtagaatccg gatcaagaca aaaatatccc tgccgctcaa actgaaaccg actacctgat
     3301 ggcgcccctc tgagaccggg ttcgacctgg caatcagaca acacggtcaa ggaatgtgga
     3361 ttgagcaggt ggtgcaggtc ctgagcgtca ccccccgaac cgggattcgg ttgagtcaac
     3421 aaggcctcat acagtctcac ttcggcagga agggcatgct gagcagagac ccagtggaga
     3481 gtccctttta cctttcgact tgattgactc ccgccacttt tcgtttcaga atcgaaagta
     3541 catcgtagcg ccgtcacttc tccggtttcg ggatccttct cgacaccgac acagcgaata
     3601 atataggcgt agcgaagacg tacttcctgc cctggagata agcggaaaaa ctttttaggt
     3661 ggatcttccc ggaaatcgtc ctgttcaata aacagctcac gagaaaatgg gatttcccgc
     3721 ttgccctggg tgggatcctc aggattattg atcgcctcta agatctcaac ctgcccatcc
     3781 ggataatttt caataatgac tttcagagga tgtaataccg ccatcacccg tggcgccgac
     3841 cggttcagat cttctcgaat agcatgctcc aacaaggcca tttcaatcac actgtcacgt
     3901 ttcgccaccc ccacccgctc acaaaaattg cgaatcgcct caggtgtgta accccgacgc
     3961 cgaagccctt tcaaggtggg caaccgggga tccccccacc cgttcacatg cttctcttcc
     4021 accaactggt gaagcttgcg tttactcatc acagtatgcc cgagattcaa acgggcgaat
     4081 tctatttgct ggggataaca cgagacctca cacacccgga gcaaccaatc atataaggga
     4141 cggtgat
//
LOCUS       DATIAL010000444         2864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_833182, whole genome shotgun sequence.
ACCESSION   DATIAL010000444 DATIAL010000000
VERSION     DATIAL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833182"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VLA60_13755"
     CDS             <1..224
                     /locus_tag="VLA60_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10472.1"
                     /translation="QQKIFGYLLDFQVAPFIDIGRVGSHFSSKTILNPQVNPGIGFQF
                     LARPHIVGRVDVAYGKDGVNVFAGLDYPF"
     gene            255..812
                     /locus_tag="VLA60_13760"
     CDS             255..812
                     /locus_tag="VLA60_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HSF10473.1"
                     /translation="MNRLPATKPIIHKYVYRLAYLVMALILSGCSLSYFNAATNGDTE
                     TVLTLLKDGIDVNTTFPIVRTHALMVAAAFGHVDTVRALIDRGADVNAKDLTGWTPLH
                     AAAFKGNMQIVRLLLEKGAVAEPSTWFLESPWVMAEELGYPAIVPLLKQAEWQTVASR
                     HPLPCIPVEFGTNYAPMPTFSQHVC"
     gene            917..1363
                     /locus_tag="VLA60_13765"
     CDS             917..1363
                     /locus_tag="VLA60_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10474.1"
                     /translation="MKHSLEHQITQAGQQPWLCRLMIAGLFSSALILGGGAAWADGDG
                     HGYGHDPKSHLEKLTKKLDLTKEQQDKILPIIEEKHQKMEGLHEQMKEMRKQAMAKIE
                     AELTPEQQAKWKEMQEEREEKMKEYKEKHGKDCDKKGKHGKDEKHD"
     gene            1783..2100
                     /locus_tag="VLA60_13770"
     CDS             1783..2100
                     /locus_tag="VLA60_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10475.1"
                     /translation="MLEVVEIRVSYRYVKEHSWVVQGITGFLSAYFMEKPGFTLKRHF
                     EELETGMHVWLCDVPPKMKVPTLLRRLKDDIPPCQYTQLETSPPAPARFLIDSLEPES
                     APS"
     gene            complement(2212..2652)
                     /locus_tag="VLA60_13775"
     CDS             complement(2212..2652)
                     /locus_tag="VLA60_13775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HSF10476.1"
                     /translation="MTEPSCKICVGAWPLKDHFLVDCGLTNAFVFEDQFFAGWTVLVL
                     KDHASELFELSRVARGMLIEEVTHVAHILAQVFDAKKINYELLGNQVPHMHWHLIPRL
                     KTDPDPLKPVWCVPHEPVRLSAEQLAERVHLLRSALRIDPNQPL"
BASE COUNT          821 a          708 c          692 g          643 t
ORIGIN      
        1 gacaacaaaa aattttcgga tatctcctgg acttccaagt cgcgccattc atcgatatcg
       61 gtcgtgtcgg atcccatttt agcagcaaga ccattctcaa tcctcaggtc aatcctggga
      121 taggattcca gttcctagcc cgtccccata tcgtgggccg ggttgatgtg gcgtatggaa
      181 aggatggagt caatgtattt gccggattgg attatccctt ttaaggtgat aaatcttttc
      241 ttgagggact gagcatgaat agacttccag ccacgaaacc catcatacac aaatatgtct
      301 atcgtcttgc ctacctggtc atggccttga ttctttcggg atgctccttg tcgtatttta
      361 acgccgccac caacggcgac acggaaacgg tgttgactct tctcaaagat ggcatcgacg
      421 ttaacaccac ctttcccatt gtgagaaccc acgcccttat ggtcgcggcc gcattcggtc
      481 atgtggacac ggtccgcgca cttattgata gaggagccga cgtcaatgcg aaggatttga
      541 cagggtggac tcccctgcat gccgcagcat ttaaaggcaa tatgcaaatc gttcgccttc
      601 tccttgaaaa aggtgcagtt gctgagcctt cgacctggtt tttggaaagt ccgtgggtca
      661 tggcagagga attgggatat ccggcaatcg ttcccttatt gaaacaggct gaatggcaaa
      721 cggtggcaag tcgccatccc ctgccctgca tccccgtgga attcggcaca aattacgccc
      781 ccatgcccac attttctcaa cacgtctgtt aatcatcttg aggatccacc acacagcttc
      841 tttcggatct gatatactag aaagttttcc cataaccgtt cacactgttt atcatttttt
      901 aatctaggag gtaggtatga agcattcact cgaacaccag attacccaag cagggcaaca
      961 accatggctg tgccgactga tgatcgccgg attgttcagc agcgcattga ttcttggtgg
     1021 cggagcagcc tgggcagatg gagacggtca tggttacggg catgatccaa aatcccatct
     1081 ggagaaactc acgaagaagc tggatcttac gaaagaacag caagataaga ttctccccat
     1141 cattgaggaa aagcaccaga agatggaggg gcttcacgaa caaatgaaag agatgcgcaa
     1201 acaggccatg gcaaaaattg aggccgaact gacgccagag caacaagcca aatggaagga
     1261 aatgcaagaa gaacgggaag aaaagatgaa ggaatacaaa gaaaaacatg gcaaagattg
     1321 tgacaaaaaa ggcaaacatg gcaaggatga aaagcacgat taagccttaa ttgagaatgg
     1381 ggggcggatc tattcactat caggtgcata tccgtccctc ttccctaacc tttcagaata
     1441 gccctgctcc cgatcctccc ctgcaaaagt aaccgcaggc cgtaatctgt ctccccttcc
     1501 atcgacccca aggatgtttg caaggtttac cggtctgtta tccacacgac tatccattgg
     1561 tatagaagac gtgacgagct caaatggaga gccagaaatc actcgggatg cccgataaaa
     1621 atcaggcatg agggtaacgg agggggacaa agccaggcat gtccggcaag acagtaacaa
     1681 aaaattccca gtggctcttc tcaatttccc aatagacgcc attattgtta aggtataact
     1741 ttccaaaaca ggatttattc gactgcacag ggagatcaag acatgcttga ggtggtagag
     1801 attcgggtca gctatcgata tgtgaaagag cattcctggg tggtacaggg catcacgggg
     1861 tttctttctg cctattttat ggaaaaaccg ggatttactc tgaaacgaca ctttgaagaa
     1921 ttggaaaccg gcatgcatgt ctggttgtgt gatgttcctc ccaagatgaa agttcccacg
     1981 ctcctgaggc ggctgaagga tgacattcct ccctgccaat atacacagct cgaaacaagt
     2041 cccccggcgc cggcgcgatt tctcatcgat tcactggagc cggaatccgc accctcctaa
     2101 ctgcccgcca aacgaaaatt ccgaagcgac tgaggacgga aggacaacag ggtaacaaca
     2161 cggaaaccgc cacgaggtga gctgcaattc atgctctcta ggctgagcaa actagagggg
     2221 ttggttggga tcgatgcgca aagccgaccg aagaaggtgg acccgttcgg ccagctgttc
     2281 agcggaaagc cggacaggct catgcggcac acaccacacc ggtttcaacg gatcaggatc
     2341 ggtcttgagc cttggaatca gatgccagtg catgtggggc acctgattcc cgagcagttc
     2401 atagttgatc ttcttcgcgt caaacacctg tgccagtata tgggctacgt gggtgacttc
     2461 ctctatcaac ataccacgcg caactctgga taattcaaac agttcggaag catggtcttt
     2521 caacaccaat acggtccagc cggcaaaaaa ctggtcctca aacacaaacg cgttggtcag
     2581 accacaatcg acaagaaaat gatccttgag cggccatgct ccaacacata ttttgcaaga
     2641 tggctcagtc acagcaacaa aaaaaacaaa gggatcaaga attcaccaat agagaaaaaa
     2701 gattataggg acaccacacg gaagagacga agccgattgg acccatgaca ccttttgtta
     2761 ttctgggaca acataccgca cattcccttc atcttgaaag gctacatcct gcatcatgtg
     2821 gtaattgaat gtgcggagaa tatatgaaaa aatgaacgga gaag
//
LOCUS       DATIAL010000445         3311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1155535, whole genome shotgun sequence.
ACCESSION   DATIAL010000445 DATIAL010000000
VERSION     DATIAL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155535"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VLA60_13780"
     CDS             <1..997
                     /locus_tag="VLA60_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10477.1"
                     /translation="PTEPSSPPPSFPFVIASSTLLGDLLLLALLRTESYHDQEFFTFF
                     RNDMASDEYKWEQFARWWTAFAGRHGVADRTEQFFRQGLGPQNALDRAAQEGGDLDYI
                     LFTLIRHWLPAVLPPAGQERTAKNDSDYWLESERILKAAVGRLRELKPVIDMLTTPSP
                     LDRAADSPRSEVEVELANMLEGIAEVVGEYGGPDYTSIIKNFDPIPLRQTQPFKHNKK
                     YSAELWVVFLLREHFRNLGLGKDRAWKLIAQLVDAANIFQSDDQPSTPEALKSWWTKN
                     WPRTYTLLTEKGQQGDAKSTAYQSDYEWFRAWFNWQTASNEFQIPSATKNPNSPS"
     gene            complement(994..1695)
                     /locus_tag="VLA60_13785"
     CDS             complement(994..1695)
                     /locus_tag="VLA60_13785"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HSF10478.1"
                     /translation="MRPCPSCSQENPDTANFCLQCGQALGSPSLETPTEANPEGLETR
                     TPPTQSQLWETFIGPSKSIQFSVKTGWVWRSAFLYYKEKFENIETPQGPRFVLSWNWP
                     AALFDSFLWFLYRKMYLFALLYAVAPALAIFMTGDVMVGIVSRILAGASANYLYYWHV
                     KDKVQRIMAMPSLDDTSRAHLIQEEGGVQPYVLWLGVVLHVLMLGLLAAAIVQGPPVQ
                     DPLGGTEGRPSQKFF"
     gene            complement(1796..2452)
                     /locus_tag="VLA60_13790"
     CDS             complement(1796..2452)
                     /locus_tag="VLA60_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10479.1"
                     /translation="MILILLWMVVVLLSYQLWVLLKRHGSFLLILKELSDRIRSGLGA
                     VDVRELKTIQAEWFRLEQQGNLRKVLGVKAGEYFQQFQECFHKGELSVDGLEPFEDSV
                     SDMNESDPRPRSIMWKGLRFTLSDSIWFQTGVVPNEDVEDDQVQSMVQGPFCRGCLKR
                     LVGRVPAQISYVPAQCHNCGLSWSIHELDYPEMPLRDLKRMVYNMLDQKVRMSRNVQC
                     "
     gene            2710..>3311
                     /locus_tag="VLA60_13795"
     CDS             2710..>3311
                     /locus_tag="VLA60_13795"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HSF10480.1"
                     /translation="MAAIEDGPLKPPLNEVEEDRALLLNTHRNWRRSLQAGIRENGQS
                     EDAQSTHTAPPSSKDFFQIDLREADFRHQDLRNANFQEAYLPGANFEGANLRGANFQG
                     ANLRSVRFRGAQLAWANFSQADLIWADFQQAELQGTDFQHATLRGATFVRAQIRGGNF
                     FSSDCSWADFQDTDLHESDFQQANLNGTTFLNAQTTGANF"
BASE COUNT          873 a          891 c          707 g          840 t
ORIGIN      
        1 accaactgag ccatctagtc ctcctccctc gttccctttc gtgattgcct cttcaacatt
       61 gttaggtgac ctactcttgc ttgcattgct tcgcaccgaa tcgtaccatg accaggaatt
      121 ttttaccttc tttcgaaacg atatggcctc tgacgaatat aagtgggaac aattcgcacg
      181 ctggtggacc gctttcgcag gccgacacgg cgtcgcagat cgaacagaac agtttttccg
      241 ccaaggcctt gggccgcaaa acgccttgga ccgggcagct caggaaggcg gtgatctgga
      301 ctatatccta tttaccctta tcaggcattg gcttccagcc gtccttcccc cagcaggaca
      361 ggaacgaact gcaaaaaacg attccgacta ttggttggag tcggaacgta tcctcaaggc
      421 tgcagtcggt cgcctcaggg aacttaagcc cgttattgat atgcttacga cacctagtcc
      481 gcttgatagg gccgctgact cccctcgatc agaagttgag gtcgaattgg ccaacatgtt
      541 agagggtatt gcagaagtgg tcggagagta tggaggcccc gattatacct caatcattaa
      601 aaactttgac ccgattcccc ttcgccaaac acaaccattt aaacacaaca aaaaatacag
      661 tgcggaattg tgggttgtct ttttattacg ggaacatttt cgaaatttgg gtttgggaaa
      721 ggatcgtgct tggaagctca tcgcccagct tgttgatgcc gcaaatattt tccagtcaga
      781 cgatcagccc tccacacctg aagcccttaa atcctggtgg acaaaaaact ggcctaggac
      841 ctacacactc ttgacggaaa agggccaaca aggggacgcc aaatctaccg cctatcaatc
      901 tgactatgaa tggtttcgag cgtggttcaa ttggcagacc gcctcaaatg aattccagat
      961 cccttcagcc acaaaaaacc ccaattctcc ttcttaaaag aatttttgag aaggacgtcc
     1021 ttccgttcca ccaagaggat cctgcaccgg aggcccttga acaatcgccg cagccaatag
     1081 gccaagcatc aacacatgca gcaccacccc taaccagagg acgtaaggtt gcactccacc
     1141 ctcttcttga atcaggtgag cacggctcgt atcatcaaga ctgggcattg ccataattct
     1201 ctgtaccttg tctttcacat gccaataata caagtaattc gcgctggctc ctgccaatat
     1261 tctcgataca attcccacca tcacatcccc agtcataaaa atggcgagag ccggggccac
     1321 agcgtataac aacgcaaata gatacatttt gcgatataaa aaccaaagaa acgaatcaaa
     1381 caacgcggcg ggccagttcc aacttaggac aaatcgtggg ccttgagggg tttcaatatt
     1441 ttcaaatttt tccttgtagt acaaaaaagc cgagcgccac acccacccgg tcttgactga
     1501 aaattgaatg gatttacttg gaccgatgaa cgtttcccat aattgggatt gagtgggagg
     1561 agtcctagtc tcgagtccct caggattggc ctctgttgga gtctctaagg aaggtgaacc
     1621 aagcgcttgt ccacattgca aacaaaagtt cgcggtatcg ggattctctt gtgaacagga
     1681 agggcaaggg cgcatagtgg tcgtttatag catcccaact acacccacac aagttctgtc
     1741 tggtagcaac agatcactag acagcatctg aatcctcctc acccatcatg aagacttagc
     1801 attgtacatt tcgactcatt cttacttttt gatcaagcat gttatacacc atacgcttga
     1861 gatcacgaag gggcatttct ggataatcga gctcatgtat gctccaggat agtccacaat
     1921 tgtggcactg cgctggaaca tatgaaatct gggccggaac cctccccacc aatcgcttca
     1981 ggcacccccg acaaaatggc ccttgcacca tggattggac ttgatcatcc tcgacatctt
     2041 cgttcggcac cacccccgtt tgaaaccaaa ttgaatcgct aagcgtaaaa cgaaggcctt
     2101 tccacatgat cgacctgggt cgagggtcgg actcgttcat gtcagaaact gaatcctcaa
     2161 agggctcaag gccatcaaca gaaagttctc ctttatgaaa acattcttga aattgctgga
     2221 agtattcccc ggctttgacc cctaaaacct tccttaaatt gccctgctgc tccaaacgaa
     2281 accattcagc ttgaatggtc ttcagctctc tgacgtccac cgctcctaac cccgatcgaa
     2341 tacgatcaga aagttctttc agtatcagga gaaatgaacc atgtcttttc agtaataccc
     2401 atagttgata gctcagaagc acgaccacca tccataatag aattaagatc acctccatat
     2461 cacctcacac atgcctaatt cttgccatgt ccgtcattga aacatttttg agtatccttc
     2521 gattcgtgtt tcatggccac aggggcgtag tatttgtata ccataattag gtttccttga
     2581 tgccttcagg ccctcatttt ggactggacc cttggtgtca ttttcgctaa ggtttcatcg
     2641 tgccctatag accagtgctc ccttttaact cattttttat tttctattgt cttttcgtgg
     2701 catttctcca tggcagcgat tgaagacggt cccttaaaac ctcctctcaa cgaggtagaa
     2761 gaggaccggg cacttcttct caacacacat aggaattgga gacgctccct gcaagctgga
     2821 atcagggaaa atggacaatc tgaagacgcc cagtctaccc acaccgctcc accaagttct
     2881 aaagattttt ttcagattga cctccgcgag gccgatttcc gccaccagga tcttcggaat
     2941 gccaattttc aagaagccta cctccctggg gcgaattttg agggagcgaa cctgcgtggc
     3001 gcaaattttc aaggagcgaa tctgcgaagt gttcgtttca gaggtgccca attagcctgg
     3061 gcgaattttt cacaagccga tcttatatgg gcggatttcc agcaagcgga acttcaagga
     3121 accgacttcc aacatgccac ccttcgaggc gccacctttg ttcgtgcaca aattcggggg
     3181 ggaaattttt tttcctcgga ctgctcttgg gccgattttc aggacactga cctgcatgag
     3241 agcgattttc agcaggccaa tctcaatgga acgaccttcc tcaatgccca aactaccggc
     3301 gccaatttcc a
//
LOCUS       DATIAL010000446         4912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_725390, whole genome shotgun sequence.
ACCESSION   DATIAL010000446 DATIAL010000000
VERSION     DATIAL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725390"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VLA60_13800"
     CDS             complement(<1..397)
                     /locus_tag="VLA60_13800"
                     /inference="COORDINATES: protein motif:HMM:NF027966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HSF10481.1"
                     /translation="MKAEPRWIRGWRLAPAHMKEGVLLICFCGVLGGCASIELPDSAE
                     QRGSEFNCAVGDVTPHEAIVWLQTTGPQTLKIQYSSDPQWSTFHETIMVSTSPETDFT
                     THIPLTALTPKTRYWYRSLVPGKDPGRPCQ"
     gene            634..1203
                     /locus_tag="VLA60_13805"
     CDS             634..1203
                     /locus_tag="VLA60_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10482.1"
                     /translation="MTPFPFLCIALLGAFHLTSREEAVFPYQADHQPSIRVQPVAMNT
                     NPFSSIQTRPKLPVEPDEFDPREINAYYDWRNDMFFREFDLTGSGSVNFMTARRTYKV
                     WLDEFGTPVVLTVGDPVFFWIDLNGNGKFEQELGEMFKDSYEDGVTGNEEPYDNSDLQ
                     EPAGPGPEWNTPLPQGGFKLPPCDSRFGC"
     gene            complement(1246..2055)
                     /locus_tag="VLA60_13810"
     CDS             complement(1246..2055)
                     /locus_tag="VLA60_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10483.1"
                     /translation="MNNNYALWTLLASLSLVMGPGASHAYEVGNVQTGGSIHGKVSFL
                     GSPPEPLHFKVEKNPEVCGQERSLMKVESRDGFLTGAVIVLEGVDTGKPFPPNTFTGN
                     LPGTGEFRYLGGESLGLQVKTKDCNFGPFTGVIAANEAVQFQNQDSSKHVLHSFVSKD
                     TRGRILRTVHNQDLQPRDAIVREFPADKMEDGVVVRIICNRHDFMQNWMYVVDNPYFA
                     ISDQEGGFTIDQIPPGTYTLLVRHPVLGEQRQEVTIEARGDLQVGFAFVDE"
     gene            complement(2125..3267)
                     /locus_tag="VLA60_13815"
     CDS             complement(2125..3267)
                     /locus_tag="VLA60_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10484.1"
                     /translation="MTRSIRYLCFGLILGTFGIVYALPPAEGALLDGFMGGVEESLGK
                     RAEEKGERLVIGNFYQAERPDSHAPIGVMQDHTHNKGEFMFSYRYMYMYMDKMRNGTD
                     NLSTNDVLKDFAVTPKNMTTQMHMFSAMYGLNDTVTLMAMLPYLMKEMEHQNRAGVNF
                     TTNSSGFGDTKLAALWRLYAFETPSIGSHRFHLNTGLSLPTGDITPTDNTPLGNNSLL
                     PYPMRLGSGTVDLLPGLTYTGVRNDVSWGFQALGTVRIGYNKQGYKEGNRYQVNSWGA
                     YRWADWISTSARLNWQQWYNIKREDGQLQTTVGPAQIPLVPTADTDRQGGQRLDLMGG
                     VNILFPEFMGLENHLNVEAGAPIYQYLDGPQLKQAWSFWVGWQMVM"
     gene            complement(3486..3785)
                     /locus_tag="VLA60_13820"
     CDS             complement(3486..3785)
                     /locus_tag="VLA60_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10485.1"
                     /translation="MKRISHLFVVCLVLCLLTLSGILTAQAVEHSQQHSQHHQGTHST
                     LLCTWFCAAGQTLETNRVLVDGPVESLLRIEDWRPITRQVIFVIPQASRAPPPCI"
     gene            complement(4191..4586)
                     /locus_tag="VLA60_13825"
     CDS             complement(4191..4586)
                     /locus_tag="VLA60_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10486.1"
                     /translation="MIVQIMQKELKTISEGCSITQAAEKMQKAGIGSLLVERKGIPVG
                     IITDTAIVHKAVGLKKNLDETKVESLMTSPLPTIQLAQTSHDALDLMGDLGVRHLIVC
                     DGPKIVGLISARDLLVYFGSVSEPRIGVD"
BASE COUNT         1236 a         1248 c         1124 g         1304 t
ORIGIN      
        1 attgacacgg tcgtccggga tcttttcccg gcaccaggct ccgataccaa taccgtgttt
       61 tcggggtcaa agccgtcagg ggaatgtggg tggtgaaatc ggtttccgga gacgttgaaa
      121 ccattatggt ttcgtgaaag gtggaccact gcgggtcgct ggagtattga attttcaggg
      181 tttgggggcc agtggtttgc aaccacacga tggcttcatg cggcgtcaca tctcccacgg
      241 cgcaattaaa ttcgctgccc ctctgttctg cactatccgg caattctatg gaggcgcacc
      301 ctcccaacac tccacaaaag cagatgagca ataccccttc tttcatgtgg gccggagcaa
      361 gcctccagcc ccttatccat cgtggttccg ctttcatcgt atggtgaatc cttttcaatt
      421 tttccagccg ggggaaatcc tctggtgaat aatacagata ggggtcggaa ggggatgaaa
      481 tccacttggt ataatttcct aaacccgtta gcgcgcgggt acttcggata agcttcaacc
      541 gggttcgatc cttcgcccaa atttgcacct ttgctcttgc aaagaataca attttgctag
      601 tctccctgaa taacgaggat aagagcaata ttaatgacgc cgtttccctt tctctgtata
      661 gcattgcttg gggcttttca tctcacttcc cgtgaggagg ctgtcttccc gtatcaagct
      721 gatcaccaac cttccatccg ggttcagcct gtggccatga ataccaatcc gttttcgtcg
      781 attcaaacac gcccaaagct tccggtggaa ccggatgaat tcgatcctcg ggagatcaac
      841 gcctattatg attggcgtaa cgatatgttc ttcagggaat ttgacctgac cggttccggg
      901 tccgttaatt tcatgacggc acgtcgaacc tataaggtct ggctcgatga gttcggcacc
      961 ccggtcgtct tgaccgttgg cgatccggtg tttttttgga ttgatttaaa tggaaacggg
     1021 aagtttgagc aggagctggg tgagatgttc aaggattcct atgaggatgg ggtcaccggc
     1081 aatgaagagc cttatgataa tagcgatctt caagagccgg cgggtccagg cccggaatgg
     1141 aatacccctc ttcctcaggg cggttttaag ctcccgccct gcgattcgcg gttcgggtgt
     1201 tgagggctat gatctcccag gaccattcac ttcttcagtt gcccttcatt catccacgaa
     1261 cgcaaatccc acctgcaaat ctccccgggc ttcaatggtg acctcctggc gctgttcacc
     1321 caacaccgga tgccggacta acaacgtata cgttcccggc ggaatctggt caatggtgaa
     1381 tccgccctct tgatcagaaa tggcaaaata cgggttatcc accacgtaca tccagttctg
     1441 catgaagtca tgccgattac aaatgatccg caccacgacc ccgtcttcca ttttgtctgc
     1501 gggaaattcc cggacgattg catcacgagg ttgaagatcc tgattatgaa ccgtgcgcag
     1561 aattcttccc ctggtatcct tggacacaaa ggagtgcagg acatgcttgc tcgaatcctg
     1621 gttctgaaac tgcacggctt cattggctgc gatcacaccc gtgaaaggac caaaattgca
     1681 atctttggtc ttgacctgaa gcccgaggga ttcccctccc aggtatcgaa attcacccgt
     1741 tccaggtaaa ttccccgtga aggtgtttgg agggaaaggt tttcccgtat caacgccctc
     1801 aagcacgatc accgcaccag tcaagaatcc atctcgagat tcaactttca tgaggcttcg
     1861 ttcctggcca caaacctctg gatttttttc taccttaaag tgtaaaggct ctggcgggga
     1921 gcccagaaaa gaaacctttc catgaatgga tccaccagtc tggacattcc ccacttcata
     1981 cgcatgggat gcaccgggcc ccatgaccag ggagaggcta gccagtaatg tccacaaggc
     2041 atagttgtta ttcatgatcc atctcctttt ttgctatttc cctagactct ccggaatttg
     2101 agccatccct acccaatctt ttttttacat gaccatttgc catccaaccc aaaaggacca
     2161 ggcttgcttc aattgcggac catcgagata ttgataaata ggcgcgccgg cttcaacatt
     2221 cagatgattc tcgaggccca taaactccgg gaacagaata ttcacacccc ccataaggtc
     2281 caaccgctgt cccccctgtc gatcagtatc ggcagtcggg acaagaggga tttgtgcggg
     2341 tccaactgtc gtttggagtt ggccgtcttc tctcttgatg ttgtaccatt gttgccagtt
     2401 caatcgcgca gacgtgctga tccaatcagc ccaacggtag gccccccagg agttgacctg
     2461 atatcgattg ccctctttat aaccctgctt attgtacccg atacgcactg tcccaagtgc
     2521 ctgaaacccc cacgacacat cattccgaac ccccgtatag gtcagcccgg gtaacagatc
     2581 gacggttccg gaacccaacc gcattggata cggcagcaat gagttgtttc ctaatggtgt
     2641 gttatccgta ggagtgatat ccccggtcgg gagcgataga ccggtattca aatgaaaccg
     2701 atgggaaccg atactgggcg tttcaaacgc atatagtcgc caaagggccg ccaacttggt
     2761 atccccgaat ccactggagt tggtcgtgaa attgaccccg gctctgtttt gatgctccat
     2821 ctctttcatg aggtagggca gcatggccat cagggttacc gtatcattca agccatacat
     2881 cgcactgaac atgtgcatct gcgtcgtcat atttttggga gtcaccgcaa aatctttcaa
     2941 cacgtcattg gtggacaggt tgtcggtccc attccgcatc ttgtccatat acatatacat
     3001 ataccggtag gaaaacatga actcaccctt gttatgcgta tgatcctgca tgaccccgat
     3061 gggagcgtgg gaatcggggc gctccgcctg gtaaaaattt ccgatgacga gtcgttcacc
     3121 tttttcttcg gcccgtttcc ctaatgattc ctccactccc cccatgaaac catccaataa
     3181 cgccccttca gcaggcggga gggcatagac gattccaaaa gttccaagga tgagcccaaa
     3241 acataaatat cgaatactcc tcgtcatatc ttgccgatcc ttttttgatt aaaattttta
     3301 gtcaatccag ccccaataaa tgataaatga atgatggcga attcattccg aatgaaatct
     3361 gtttggggaa agggtctttc cacagagcaa aattccgctc gcaagctttt ctagccaagg
     3421 cttgcgaatg aaaccctctt aacttccgaa ggttttttat aaccaagaaa ttcgtaatga
     3481 taaagctaaa tgcagggagg aggggcacgg gaagcttgag gaataacgaa aataacttga
     3541 cgggtgattg gacgccaatc ttcaattcga agaagagact caaccggtcc atccactagc
     3601 accctgttag tttctagtgt ttgccctgca gcacaaaacc aggtgcacag aagtgtggaa
     3661 tgggtccctt gatggtgttg agaatgctgt tggctgtgct caacagcctg ggcggtgagg
     3721 atgccactca gggtcagtaa acataaaacc agacagacaa caaataggtg agaaatacgc
     3781 ttcatgttgt gagagcataa aattctttac ggcctataat tttagtaaca gataaggaga
     3841 ccccacttca accctatgag gatggggcat ttttctacaa aggccgcata aaaatggctt
     3901 aagaatgacc aacggaggtt ttgggagatc tgaaatgttt tggacgaaga gaccactcaa
     3961 gaattgcgag tcctgccctt tacgggagac tcagccgtat cgactctggg agtactctct
     4021 taatttttga aagatgaaca ccttgttaat ggcagtgatt taaggaaacc tccttccttg
     4081 ccaggcgtaa aaatctcgtg ggcaaaatat ccgaacaacc ttaattgttc gcgagtcttt
     4141 ccctcaaaaa aatacaaaaa ggtattattt atgccagctt atccgctgga ttaatctacc
     4201 cctattctgg gttccgagac acttccaaaa tacaccagaa gatcccgtgc agaaattaaa
     4261 ccgacgattt tcggtccatc acatacaatg agatggcgaa cgcctaagtc acccatcaaa
     4321 tccaacgcat cgtgggaagt ttgggccaat tggatagtag ggagaggtga ggtcatgaga
     4381 ctctctacct tggtctcatc caggtttttc ttgaggccaa cggctttatg gacaatagct
     4441 gtatcagtaa tgattccgac aggaattccc ttccgttcaa ccaacaagct tccaatccct
     4501 gctttctgca tcttttcggc cgcttgagtg attgaacaac cttcagaaat ggttttcaat
     4561 tctttctgca taatttgaac gatcatgtat ggttcccttc cgatgatggt gtgtgtaatt
     4621 tctctatatt ataccaattt tttcaatagc gtcaccgttc tttttttcat gctaattaaa
     4681 cgtcaatcga ggaggcagga ttggacattc ccgagccaga ttcatcctat ccaggccgga
     4741 acagctccgg gaactcacaa tgaaaaacaa aattcttttg cgaattattg acacttcgag
     4801 aagtgggcat tgtccaattt ttcgaaatag caatataggc cttgggctcc ttcgcgtgtc
     4861 gtttcctatt ttttactaag agttaatgga cggatttttt ttcacaagtc tt
//
LOCUS       DATIAL010000447         4409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_80805, whole genome shotgun sequence.
ACCESSION   DATIAL010000447 DATIAL010000000
VERSION     DATIAL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80805"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..502)
                     /locus_tag="VLA60_13830"
     CDS             complement(89..502)
                     /locus_tag="VLA60_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HSF10487.1"
                     /translation="MGGSNPYIEKSETAVAKKPFKLTFITPEKPIEIEVDPEKFPYGE
                     TGLPGSILDIALGNGIDVEHACGGVCACSTCHIIVKQGLDTCNEATDDENDQLDEAPG
                     LTLQSRLGCQCVPNGEKDLIIEIPAWNKNLVKESH"
     gene            707..1192
                     /locus_tag="VLA60_13835"
     CDS             707..1192
                     /locus_tag="VLA60_13835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF10488.1"
                     /translation="MLKLSKKADYALMALQYMAMVRSGETSPFNPNRVVNTKEIAEEH
                     HIPLELLAKVLQTLAKYDMVESQNGPKGGYLLAREPREISIAQVLEAIEGPLGIADCY
                     HEKDEHASCEQIEHCNIRTPLLRVQESIFHLLSSMSIEDMMAEPPLIIVESRKTKGVQ
                     L"
     gene            1189..2403
                     /locus_tag="VLA60_13840"
     CDS             1189..2403
                     /locus_tag="VLA60_13840"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HSF10489.1"
                     /translation="MKLPIYLDNHSTTPCDPRVLEMMLPYFTEKFGNAASRNHTFGWE
                     AEEAVEVARKQIAHLIQADAKEIIFTSGATESDNLALQGVVEMYREKGNHIITSSTEH
                     RAVIDTAKYLEKKGIKVTFLPVDKAGMVSPDDVRNAITDQTILISIMMANNEIGTINP
                     VAAIGKVAKEKGVLFHCDATQGVGKIPVNVQEMGIDLMSFTAHKIYGPKGVGALYVRR
                     KAPRVRLEAMMYGGGHERGMRSGTLAVPLIVGFGKACELCEQEMSTESVRMAKMRDRL
                     QEGIMKGMDEVYLNGHPTERLPNNLNISFAYVEGEALLMGVKEIALSSGSACTSATLE
                     PSYVLRALGVGSDLAHSSIRFGLGRFNTDEEVEYTIDRMIKAVTHLREMSPLYEMAKE
                     GVDLKSVQWAAH"
     gene            2486..2902
                     /gene="iscU"
                     /locus_tag="VLA60_13845"
     CDS             2486..2902
                     /gene="iscU"
                     /locus_tag="VLA60_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HSF10490.1"
                     /translation="MAYSEKVIDHYNNPRNVGSFQKDADDVGTGVVGAPECGDVMKLQ
                     IKVENDTIVDAKFKTFGCGSAIASSSLATEWLKGKTLEDAQKIKNTDIVHELNLPPVK
                     IHCSVLAEDAIKAALGDYQKKAGEKKVKPEAATTSS"
     gene            2944..3297
                     /locus_tag="VLA60_13850"
     CDS             2944..3297
                     /locus_tag="VLA60_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HSF10491.1"
                     /translation="MDTKTTESNSTVAMTDEALKEVKRLLDLQGITEGGLRLGVKGGG
                     CSGLSYTVNFDDKIGEFDTVTEVDGVKVIVDAKSAIYLQGMTLDYQKDMVSGAFKFIN
                     PNATKTCGCGESFSA"
     gene            3355..4104
                     /gene="hscB"
                     /locus_tag="VLA60_13855"
     CDS             3355..4104
                     /gene="hscB"
                     /locus_tag="VLA60_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HSF10492.1"
                     /translation="MPELSEPEIMEHQHQTTTKARELPMARSMCWHCQSEVTGEYLCG
                     QCVKVQPLSKDLDYFACFQLPRLLNIDEQQLEQTFYDLSRTFHPDFYSTKDESEKAIS
                     LGNSAFLNAGYRTLKDPIQRAEYLIRLEAGAVKDIRSNPPADLFEEVLELQEDLETFR
                     QLAPKGESPDLEALRKKLQDKRERLEKRQAKMEESLQSQFKEWDQLQARTPLPGEARE
                     EKNFILRAMQEILSNRTYVRNMVNDLLETTG"
     gene            4131..>4409
                     /locus_tag="VLA60_13860"
     CDS             4131..>4409
                     /locus_tag="VLA60_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HSF10493.1"
                     /translation="MSRIVGIDLGTTNSLIAFMDKEGPHVIPGPDGQNKVPSIVGLTD
                     NGMIVGEPAKAHLIRDPSHTIYSVKRFMGKGLADVKDDLKYFPYQLHEK"
BASE COUNT         1214 a          933 c         1124 g         1138 t
ORIGIN      
        1 tgttcgaaca atttcgtacg ggaaaatgtg gggagagtgc aagagcacat ccttctcacc
       61 cgtgtcttgt ataaacaacc gtgttccctt agtgactttc tttgacaaga tttttattcc
      121 atgccgggat ttcaatgatg aggtcttttt ccccattcgg gacgcactgg caccccaatc
      181 ttgattgaag ggtaagaccg ggggcttcat ctaattggtc attttcgtca tccgtggcct
      241 cgttgcaggt gtccagcccc tgcttcacaa tgatatgaca ggttgagcag gcacacactc
      301 cgccgcatgc atgttccaca tctatcccat ttcccaacgc gatatcgagg atgctcccag
      361 gcaaacctgt ctccccataa gggaattttt ctggatcaac ctcaatttca atgggctttt
      421 caggtgtaat aaaggtcagt ttgaatggtt ttttagcgac tgcggtttcg cttttctcaa
      481 tatatggatt actgcctccc atttgattct ccttataaat caatgcgttg aaaatttatt
      541 tttttaatta gttcgttggt gattattttt gtccttacta attccgacta aatggatctc
      601 cgaccatttt agtcttgact gacacaagag gtcaatgata cattaaaccc gatcatataa
      661 gtcggcatta tggctaaata aatactataa atattaagac ttaactatgc tgaagctctc
      721 gaaaaaagca gattatgccc tgatggctct ccaatatatg gccatggttc gatctgggga
      781 aaccagtcct tttaatccga atagggtcgt taacaccaag gaaatcgctg aggaacatca
      841 tattcccctg gaattgttag ctaaagtgct tcaaaccctg gctaaatatg acatggtgga
      901 aagtcaaaac ggaccaaagg gaggctacct cctggcccgt gagcctcggg agatttcgat
      961 tgcgcaggtg ctggaagcga ttgaagggcc actgggaatt gcagattgtt accatgaaaa
     1021 agatgaacat gcctcctgtg aacaaattga gcattgtaat atccggactc ctctgttgcg
     1081 ggtccaagaa agcatttttc atttactcag tagcatgtct attgaagata tgatggccga
     1141 gcccccctta atcattgtgg aatctcgtaa aacaaaagga gttcaattat gaagttgcct
     1201 atatatttgg ataatcattc aacgaccccc tgcgatcctc gggtgcttga aatgatgctc
     1261 ccctacttca ccgagaaatt tggaaatgcg gccagccgga atcatacttt cggttgggaa
     1321 gccgaagagg cggtggaagt ggccagaaag cagattgcgc atctaatcca ggccgatgcc
     1381 aaagaaatta ttttcaccag tggggcaacg gaatcggata atctcgcctt gcagggcgtg
     1441 gtcgaaatgt accgggaaaa aggaaatcat atcattacaa gttcgacgga acaccgggcg
     1501 gtgattgata cggcgaaata tttggaaaag aagggtatca aggtcacttt cctccccgtt
     1561 gataaagccg gcatggtgag ccctgatgat gtacgcaatg ccattaccga ccagacgatt
     1621 ttaatttcga ttatgatggc caacaatgag atcggtacaa tcaatccagt ggcggccatc
     1681 ggaaaagttg caaaagaaaa aggtgtcctc ttccactgtg acgcgacaca aggggtgggg
     1741 aaaattcctg tgaatgttca ggaaatggga attgacctga tgtcctttac cgcccacaag
     1801 atttatggtc ccaaaggcgt gggggcccta tatgtccgac gtaaggcgcc aagagtgagg
     1861 ttggaggcga tgatgtatgg tggaggtcat gagcggggta tgcgatcggg aaccctggct
     1921 gtgccgctca ttgtcggatt tgggaaagcc tgcgaactat gtgagcagga gatgtcaaca
     1981 gagtctgttc gtatggccaa gatgcgcgat cgccttcaag aaggcattat gaagggtatg
     2041 gatgaggtct atttaaacgg gcatccgact gagcggttgc cgaacaatct caatattagt
     2101 tttgcgtacg tcgaaggaga ggcgttgctc atgggggtca aagaaattgc gttgtcttct
     2161 ggttcggctt gtacgtcggc cacacttgag ccctcttatg tgttgcgtgc cttgggagtt
     2221 ggatcggatt tggcccattc ctcgatccgc tttgggcttg gtcgatttaa taccgatgag
     2281 gaggtggaat acaccattga tcgcatgatc aaggctgtga cgcacctacg cgagatgtcc
     2341 ccgctctatg agatggccaa ggagggggtc gatttgaaaa gcgttcaatg ggcagctcat
     2401 taattaattg atacgactga agatatattc acaatggttt taaaaaatac actgtcatac
     2461 catacaacaa agggagggat agtccatggc ctatagtgaa aaagtcattg atcattacaa
     2521 caatccccgt aatgtgggaa gttttcaaaa agatgccgac gatgtcggaa caggtgtcgt
     2581 cggagccccg gaatgcggag atgtgatgaa actccaaatt aaagtcgaaa acgacactat
     2641 tgtggatgcc aagttcaaga cgtttggctg tggctcggcc attgccagtt ccagtttggc
     2701 gaccgagtgg ctcaagggga aaaccttaga agatgctcaa aagattaaga atacggatat
     2761 tgttcatgaa ttgaacctcc cacccgtaaa aatccattgt tcagtgttgg cggaagatgc
     2821 cattaaagcg gccttggggg attaccagaa aaaagcggga gagaagaagg ttaagcccga
     2881 agcagccact acctcatcct agcttaggga actgttcacg atttgttgta gggagaaatg
     2941 gcgatggata caaaaaccac ggaaagcaat tcaacggttg ccatgactga tgaggcgttg
     3001 aaagaggtca agcggttatt ggaccttcag ggcattactg aaggtggtct tcgattggga
     3061 gtcaaaggtg gcggctgttc ggggttgagc tataccgtca acttcgatga caagatcgga
     3121 gagtttgata ccgtcactga agtggatggc gtaaaagtga ttgtcgatgc caaaagcgcc
     3181 atatatctcc aggggatgac cttggattat caaaaggaca tggtgagtgg tgcctttaaa
     3241 tttatcaatc ctaatgccac caagacatgt gggtgcggag agtcgttttc ggcgtaagac
     3301 taattttctt caatcacata gtgacgcaat aactcgggca tggggcagtt caccatgccc
     3361 gagttgtcag agccagaaat catggaacac caacatcaaa ccacgacaaa agcccgagaa
     3421 cttcccatgg cccgcagtat gtgctggcac tgccaatctg aagtcacggg agaatatctc
     3481 tgtgggcaat gcgtcaaagt tcagcccctt tcaaaggact tggattattt tgcgtgcttt
     3541 caacttcctc gtttgttgaa cattgacgaa caacaattgg agcagacatt ttatgattta
     3601 agtcgtactt ttcatccgga tttttattcc acaaaagatg aatcggagaa agccattagt
     3661 ttagggaatt ctgcatttct gaatgccggc tatcgtacac tcaaggatcc cattcagcgg
     3721 gcagaatatt tgattcggtt ggaagctgga gccgtgaaag atattcgatc gaatcccccg
     3781 gcggatttgt ttgaagaagt tcttgagctg caagaggatt tggaaacatt ccgacaactg
     3841 gcccccaagg gcgagtcacc tgaccttgag gcgcttcgga aaaaattaca agacaaacga
     3901 gagcgtttgg aaaaacgcca ggccaaaatg gaggagtccc tgcaaagtca gtttaaagaa
     3961 tgggatcagt tgcaagcacg cactcccctt ccaggtgagg cgcgtgagga aaaaaatttt
     4021 attttgcgtg ctatgcagga aattctttct aatcgaacat acgtgcggaa tatggtcaac
     4081 gatttgctcg aaaccacggg ctaatcttca tcagctaaaa ggaatatatt gtgagtcgca
     4141 ttgttggtat tgatttggga acgacgaatt cgctgattgc ctttatggat aaggaggggc
     4201 ctcatgtcat ccccggcccg gacgggcaga acaaggtgcc atccatcgtc gggttaaccg
     4261 ataatgggat gatcgtggga gagccggcaa aagcccattt aattcgtgat ccttcccaca
     4321 ccatttattc tgtgaagcgg ttcatgggca aggggctggc tgacgtgaag gacgacctga
     4381 agtattttcc ttaccagctt catgaaaaa
//
LOCUS       DATIAL010000448         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1587037, whole genome shotgun sequence.
ACCESSION   DATIAL010000448 DATIAL010000000
VERSION     DATIAL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587037"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VLA60_13865"
     CDS             <1..569
                     /locus_tag="VLA60_13865"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HSF10494.1"
                     /translation="ELQSLLALPQIPTRIECFDISNTMGTHSVASMVVWEQGRMKKSD
                     YRRFRIKTVEGANDFASMHEVVKRRYGGSLANKPGKTLPEPDLVVIDGGAGQLGAAME
                     AMAAVGLSQVAICGLAKAKGDKDERIFLPGRKTPIILPLKSPATRLVQTIRDEAHRFA
                     ITFHRKLRGDAMIPLPLLRSAKSSKGSS"
     gene            908..1156
                     /locus_tag="VLA60_13870"
     CDS             908..1156
                     /locus_tag="VLA60_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10495.1"
                     /translation="MQCPRCQGTMIVDHFVDMATSGEIWMPGWRCLMCGEVLDPLIEH
                     HRHLQREHQEVVGAISGSTARPPSPISVKSRKANKRSF"
     gene            1469..>2148
                     /locus_tag="VLA60_13875"
     CDS             1469..>2148
                     /locus_tag="VLA60_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HSF10496.1"
                     /translation="MAHTYDHQAVEAKWQAYWAQSGTFQVQEDSDKPKYYCLEMFPYP
                     SGRIHMGHVRVYAIGDVVARYKTMRGYNVLHPMGWDAFGLPAENAAIEKGVHPNVWTQ
                     ENVAYMKGQLQRMGLSYDWDREVNTSQPEYYRWNQWIFVKMVERGLAYRKRSAVNWCL
                     SCETVLANEQVLAQEGKDGGVCWRCESVVIQKELEQWFFRITSYSQELLDGCERLATW
                     PARVLAMQR"
BASE COUNT          485 a          469 c          625 g          569 t
ORIGIN      
        1 aagaacttca gtcgctctta gcgttgccac agattccgac ccgcattgaa tgttttgata
       61 tctccaatac catggggacc cattcagtgg cgtccatggt ggtttgggaa caaggcagaa
      121 tgaagaagtc agactatcgg cggtttcgga ttaaaacggt agaaggagcc aacgactttg
      181 ccagtatgca tgaagtggtg aaacgccgat acggcggatc gttggctaac aaaccaggga
      241 aaaccttgcc ggagccggac ctggtggtga ttgatggtgg agccggacaa ttaggcgcgg
      301 ccatggaagc gatggcggcc gttggtctct cacaggtcgc gatttgtggt ttagccaaag
      361 cgaagggtga caaagacgaa cggatttttc tgccgggtcg aaagacgccc atcatactgc
      421 ccctgaaatc accggcaaca cggctggtcc aaaccattcg agatgaagcg catcgctttg
      481 ccatcacctt tcatcgcaag ctgcggggtg acgcgatgat tcctcttcct ctcctgcgtt
      541 ccgcaaaatc ttcgaagggg agttcctaat ccttcgccag atatggtgtt ggtgtcggct
      601 gtggttgtcg atgcccacca attctcgcaa aatgtgatct gggacttctt ggaaaacagg
      661 tgcggacgga ttccgaactg ctgcgtggga atcttgtttg gagcaagtgg gtttttcgtt
      721 tccttgaaaa aattgagatg gctgtgatga gacaggggga gcatatgtgt tttcttgagg
      781 ttgtattgtc ctgttgccca ttattcgacg aacacgagac ttccccttct cccctttgaa
      841 agatttaagg tctgtgaggg cctcaccgat ggatacatac atcacgtata aagaggaggt
      901 atgacggatg caatgccctc gatgtcaggg gaccatgata gtcgatcact ttgtggatat
      961 ggccacgagt ggcgagattt ggatgccggg ttggcggtgt ctgatgtgtg gagaagtcct
     1021 tgatccgtta attgagcatc atcgtcatct gcagcgtgaa catcaggaag tggttggtgc
     1081 catttccgga tcaaccgctc gtcctccctc acccatttcc gtgaagtcgc gcaaggcgaa
     1141 caagcggtcc ttttagtcgt tctgttcttc ccctttttcc tgtgcattta ttcgcgatcc
     1201 ggtggtctga acggcctctt tgcgatttgc ttgtggggtg ggtttctcta tgctagattc
     1261 ctcaggatgc tgttgaaaat gcagcgtttc ccaacgtcaa atccggaggc aatgacccgc
     1321 atagtttccg aatgtcttta tccaatttga cgcggaaaaa gtttttaccc atacggacat
     1381 ttctttacac atgtggcgag gaagtaggga ttagtttgtg gcgtattttt cgaggcgatc
     1441 accgtatttt ctttcataag gcataagcat ggcgcatacg tacgatcatc aggctgttga
     1501 agcaaagtgg caggcatatt gggctcaatc cgggacgttc caggttcagg aagattcgga
     1561 caagcccaaa tattattgtt tggagatgtt tccctatcct tcgggccgga tacacatggg
     1621 ccatgtgcgc gtgtatgcca tcggggatgt cgtagcccgc tataagacga tgcgtgggta
     1681 taacgtgttg catccgatgg gatgggatgc tttcgggttg ccggcagaga atgccgcgat
     1741 tgagaaaggg gtgcacccca acgtctggac tcaagaaaat gtggcctata tgaaagggca
     1801 acttcagcgg atgggtctgt cctatgattg ggatcgtgag gtgaatacgt ctcagcctga
     1861 atattaccgt tggaatcaat ggatctttgt gaaaatggtt gagcgtggat tggcatatcg
     1921 aaaacgctcg gcagtgaatt ggtgtctttc ttgtgaaacg gtcttggcga atgagcaggt
     1981 gttggcgcaa gaaggaaaag acggaggcgt gtgctggcga tgcgagtcgg tcgtcattca
     2041 aaaagaattg gagcagtggt ttttccggat tacgtcctat tcgcaggaat tattggacgg
     2101 ctgtgagcgg ttggcgacct ggccggctcg tgtgctggcc atgcagcg
//
LOCUS       DATIAL010000449         5679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1156036, whole genome shotgun sequence.
ACCESSION   DATIAL010000449 DATIAL010000000
VERSION     DATIAL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156036"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /gene="nusB"
                     /locus_tag="VLA60_13880"
     CDS             complement(<1..462)
                     /gene="nusB"
                     /locus_tag="VLA60_13880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSF10497.1"
                     /translation="MTMTIPSDTPQANQAHGRPSRRLARQLALQMLFQHEFQDQNPTW
                     QEKFWASQSASPEVQTFTSNLFLGVITNQSEIDRIIRDFAIDWSLQRMPVVDRNILRC
                     SIYELLWEPDIPAMVTINEAVELAKLFADDEARRFVNGLLDHVLRAEPKLAI"
     gene            complement(459..968)
                     /gene="ribH"
                     /locus_tag="VLA60_13885"
     CDS             complement(459..968)
                     /gene="ribH"
                     /locus_tag="VLA60_13885"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HSF10498.1"
                     /translation="MANQERGDKADCQVAIVVSTFNEIVTSRLLAAAEFTLTQLGTPP
                     DHRQVIRVPGAFEIPLIAKTLAQSHRFDAIICLGAVIRGETPHFEYICAETSRGIGQA
                     SLETGIPIIFGVLTTDTVDQAMERSGPPERNKGADAARTALEMAVLMKRLSKTSIRQS
                     GFLRESTTS"
     gene            complement(1077..2306)
                     /locus_tag="VLA60_13890"
     CDS             complement(1077..2306)
                     /locus_tag="VLA60_13890"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HSF10499.1"
                     /translation="MKKETAPIHQPQFHTIAETLQDIQAGKCIILVDDEDRENEGDLV
                     MAAEKVTPEHINFMAKYGRGLICLALTPDRVEELKLPPQANENTAPFGTAFTVSIDAA
                     KNITTGISAADRATTIQLAVDPTAKPTDFARPGHIFPLKANKGGVLRRAGQTEGSVDL
                     ARLAGLYPAAVICEIMNDDGTMARVPHLMKFAKHHGLKIITIKDLIQYRLHRETFVKQ
                     VATANLPTRFGHFQSVVFENELDAGTHIALVKGEIDSLPTLVRVHSGCLTADVFGSLR
                     CDCREQLHRAMEIIEREGKGVLLYLNQEGRGIGLTNKIRAYHLQDQGSDTVEANLQLG
                     FKADLRDYGIGAQILVNLGLKKIRLITNNPRKIVGIEGYGLEVVERVSIEITPQESNV
                     HYLRTKKAKLGHLLNNV"
     gene            complement(2348..3571)
                     /locus_tag="VLA60_13895"
     CDS             complement(2348..3571)
                     /locus_tag="VLA60_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF10500.1"
                     /translation="MLKEVIQSLRSGHWPTLLGAWLHFEISFMVWLLIGAMGIAISEE
                     FSLSSSQKGLLVGFPLLGGALLRIIVGPLGDRLGAKVVGLAILGLEGIGLLLGWLGGT
                     SFESMLGIGLFLGFAGASFAIALPLASQAYPSAHQGLAMGVVAIGNSGVLLAAFMAPR
                     LAEGVGWHQVFGIMLLPVMGAALLFSWLVQSAPIRNETDLTKNDSIGIFLRKGIQDPV
                     MYWLCFLYGVTFGGFVGLSSYLPIFLHDQFHVGMVKAGTLTAISALAGSVVRPLGGYL
                     ADRHGGIALLQGLYLIIAALCLVSGNLDSFVLTYAMILLIMLCFGFGNGAIFQVVSCR
                     FKSVMGTASGFVGAAGGLGGFLLPYGFGWLKELTGTFSSGFYTLGLVSGLAGISVMMI
                     QRSIRLTKHKSISEI"
     gene            3812..4852
                     /locus_tag="VLA60_13900"
     CDS             3812..4852
                     /locus_tag="VLA60_13900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HSF10501.1"
                     /translation="MTQKSSKKRTPDVIDVVVKKSLSEQPNEEVHEEIILDDAKEQEN
                     QPESEEASKDQAKWDSVSTALTPTTTLSRYLAEVRRYPFLSKEEELQLFHEYQTLGTR
                     ESAVKLILANLRVCVSIASEYGLAGIDQMDLIQEGNVGLLQAMKKFDPTKNVRFYAYA
                     AWWVRAFVLRYLLNNFRLVKIGTTQEQRRLFYNLRREKAKLERQGYVPDPKLLADRLN
                     VRERDVVEMDQRLGSWELSLDQPMTSDGEGTFHDLLPSTQAPIDDQLADTQLRLLFRK
                     KLAEFAQTLSEREEDILRNRLLSESPVTLEDLGRKYMITKERTRQLEAKIIKKLRELM
                     KKDIQDFEHLRK"
     gene            5105..5422
                     /locus_tag="VLA60_13905"
     CDS             5105..5422
                     /locus_tag="VLA60_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HSF10502.1"
                     /translation="MAATKEKEKKEAKEGSSAKGFRPLGDRVFVAYTEELERTAGGIY
                     VPDSAREKPQRGTIEAAGPDVENVKVGDQVLFDKYSGTKIKVENDDCLILKEEDILGV
                     FEN"
     gene            5497..>5679
                     /locus_tag="VLA60_13910"
     CDS             5497..>5679
                     /locus_tag="VLA60_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HSF10503.1"
                     /translation="MAKQLQYSEKARASILSGVNQLADAVKATLGPKGRNAILDKKFG
                     APTITKDGVTVAKEIEL"
BASE COUNT         1622 a         1386 c         1285 g         1386 t
ORIGIN      
        1 gatggcaagc tttggttcag cccggagcac atggtctaac agtccattga cgaatcgtct
       61 ggcctcatca tcggcaaata gcttagctaa ttcaaccgct tcattgatgg tgaccatggc
      121 cggaatatcg ggctcccaca ataactcgta aatggaacat ctcaggatat tcctatccac
      181 caccggcatc cgctgcaaag accagtcgat ggcaaaatca cgaatgatcc ggtcaatttc
      241 ggactgattc gtgatgacac caagaaagag gttagaggta aatgtttgga cttccggtga
      301 agccgattga cttgcccaaa acttttcttg ccatgtaggg ttttgatcct gaaattcatg
      361 ctggaagagc atttgcaacg caagctgacg ggccaggcgt ctggatggcc gtccatgtgc
      421 ttgattggct tggggtgtat cggatggaat agtcatggtc atgatgttgt ggattccctt
      481 aaaaagccag actgacggat gctggtctta ctcaaacgtt tcattaagac ggccatttca
      541 agagccgtac gggctgcatc agccccctta ttccgttcgg gtggcccaga acgttccatt
      601 gcctgatcaa ccgtatccgt agtcagcact ccgaaaataa ttggaatgcc agtttccaga
      661 gaggcttggc cgatcccgcg acttgtctcg gcacaaatat attcgaaatg tggagtttct
      721 ccgcggatta ctgcccctaa acagataatg gcatcaaatc gatgtgattg ggccagtgtc
      781 tttgcaatta aaggaatctc aaatgctcca ggaacccgaa tgacctgcct gtggtctggt
      841 ggagtgccta gttgggttaa ggtgaattca gctgcagcca ataagcggct ggtcacaatc
      901 tcgttaaaag tactgaccac gatcgcaacc tgacagtctg ccttgtcacc acgctcctga
      961 ttcgccatgt tggtttaata attcctttga gattcaccct ctcactacaa aaagtaagag
     1021 ccttgcgatt cgcgattcag tgtgtaccac ttgccagaaa gaactgcaag tccccactac
     1081 acattattca aaaggtggcc taatttggcc tttttcgtgc gcaagtagtg cacatttgac
     1141 tcctggggag taatctcaat ggacactcgt tcgaccactt ccaatccata accctcgatt
     1201 cccacaattt tccttggatt attggtgatg agtctgattt ttttaagacc taaatttact
     1261 aaaatttggg cgccaattcc gtaatcccgc aaatcagcct taaaccccaa ttgcaaattg
     1321 gcttcgactg tatcactgcc ctggtcctga aggtggtagg cccgaatttt attggtcagt
     1381 ccgattcccc gtccctcctg gtttaagtac agcaggaccc ctttgccttc ccgttcaatt
     1441 atttccattg ctcgatgcag ttgctctcgg caatcacagc gcaaagaccc aaagacatcg
     1501 gctgtcagac agcccgaatg gactcgtacc aacgttggaa ggctatcgat ttcccctttc
     1561 accaaagcta tgtgtgttcc ggcatcaagt tcgttttcaa aaacgaccga ctgaaaatga
     1621 ccaaaacgtg tgggaagatt cgcggtggca acttgcttca caaacgtttc tcgatgcaat
     1681 cgatattgaa tcaaatcttt aattgtgatg atcttcaggc catggtgctt tgcaaatttc
     1741 attaagtgag gaacccgtgc catcgtacca tcatcattca taatttcgca gatcactgcc
     1801 gcgggataca gcccggccag ccgggcaaga tcaacagacc cttcggtttg tccggcacgg
     1861 cgcaatactc cgcctttatt ggcttttaaa ggaaagatgt gtccaggtct ggcaaaatcg
     1921 gttggcttgg ctgttggatc cacggccaac tgaatcgtgg tagctcgatc agcagccgag
     1981 atccccgtgg taatattctt ggcggcatct atcgatacag taaaggctgt gccaaacggt
     2041 gcggtatttt cattcgcctg gggggggagc ttcaactcct caactcggtc gggggtgagg
     2101 gccaggcaaa ttaatcctcg gccgtatttc gccatgaagt taatatgttc aggcgtgacc
     2161 ttttcagctg ccatgaccaa atcgccctca ttttcccgat cctcatcgtc aacaagaatg
     2221 atgcatttgc cagcttgaat atcctgtaaa gtttcggcaa tagtgtgaaa ttgaggttgg
     2281 tgtattgggg ccgtttcttt tttcatgggt gactcccttg acgggcaaac gtttcaggca
     2341 cagctcgtta aatttcggaa atggatttgt gtttggttaa ccgaatcgag cgctggatca
     2401 tcataacact aatgcctgct aatcctgaaa ccaagcccag ggtgtaaaat ccagaggaga
     2461 aggttccggt taattccttt aaccatccaa acccgtatgg taagaggaat cctccgaggc
     2521 ctcctgcagc accaacaaag ccggaagccg tacccattac ggatttgaat cgacaggaaa
     2581 caacctggaa aatggctcca tttccaaatc cgaagcaaag cataattaga agaatcatgg
     2641 cataggtcaa gacaaaacta tcaagattcc cggaaaccag gcatagggcg gctattataa
     2701 gatagagccc ttgcaataag gcgatgcccc cgtggcgatc cgcaagatat cctcccagag
     2761 ggcggaccac acttcctgct agagcggaaa tcgcggtcaa tgtcccagcc tttaccatgc
     2821 cgacgtgaaa ttgatcgtga agaaaaatcg ggagataact tgaaagccct acaaatcctc
     2881 cgaacgtcac accatacaaa aaacataacc agtacatgac tggatcctga atcccttttc
     2941 gcaaaaatat tccaatactg tcattttttg taagatcggt ttcattcctt atcggagctg
     3001 attgaaccaa ccaggaaaac aggagggctg cccccataac gggtaggagc atgataccga
     3061 aaacctgatg ccatccaacc ccttccgcaa ggcgtggagc cataaacgca gccaacagca
     3121 cgccactatt tccaatcgcc acaaccccca tggccaaccc ctgatgggct gagggatagg
     3181 cttggctggc caggggcaga gcaatcgcga aactcgcgcc agcaaatcct aaaaagagtc
     3241 caattcccag catagactcg aaactggttc cacctaacca tcccaacagt aacccgatgc
     3301 cttccaagcc cagaatggcc aatcccacaa ctttagcccc taatcggtct cctagcggcc
     3361 ccacaataat tcgaagcaag gcgccaccca gcaatgggaa gccgactaat aggccctttt
     3421 gagaagacga aagagaaaat tcttcagata tagcaatacc catggcccca ataagaagcc
     3481 acaccataaa acttatttca aagtggagcc acgcaccgag gagagttggc caatgcccgg
     3541 aacgaagtga ctggataacc tctttcaaca tagttctttc aagggcatgg gaggcctttc
     3601 tgtttaaatt tttcaagcct gtgatcaaag gctgactata aaaatattta tctgaaatag
     3661 acaatatttc tctgaagtgg aattcctgaa acctcttttt cacctcgaaa ttggaaaaat
     3721 tcgtcaatgc gtttggtgat ccaagaaaaa ttcattacaa taaagcagaa gaatgcggca
     3781 tcccctgcct tctggatcag caagccaaag catgacacaa aaatcttcca aaaagcggac
     3841 tcccgatgta atcgacgtcg tggtgaaaaa atctctttct gaacaaccga atgaagaggt
     3901 ccatgaagaa atcatccttg atgatgccaa ggaacaggag aaccaacctg aaagtgaaga
     3961 ggcctccaaa gaccaggcga agtgggattc ggtctcaacc gcactgactc cgaccacaac
     4021 actcagtcga tacttagccg aagtccgacg gtatccattc ctatcgaaag aagaagagct
     4081 tcaactcttt catgaatatc agacactagg gacacgagag tctgcagtga aattgatttt
     4141 agctaatctg cgtgtgtgtg tctccatcgc ctcggaatat ggtcttgccg gcattgacca
     4201 aatggacctg atccaggaag ggaacgtcgg gcttttgcag gccatgaaga aattcgatcc
     4261 gacaaaaaat gttcgctttt atgcctacgc agcctggtgg gttcgagcct ttgtgctgcg
     4321 gtatctcctc aataattttc gtcttgtcaa aataggcaca acccaggaac aacgacgcct
     4381 tttctataac cttcgtcgcg aaaaagccaa attggaacga caaggctatg ttcccgatcc
     4441 aaagttgctg gcagaccgcc ttaatgtgcg tgaacgagat gttgtggaaa tggatcaacg
     4501 cttaggaagt tgggaacttt ccctcgatca gccaatgacc tcggacgggg aaggcacatt
     4561 tcatgacctt cttccttcta cacaagctcc cattgatgac cagctggctg acacacagtt
     4621 gcgcctcctc ttccgaaaaa agttagctga atttgcacaa acactatcag aacgagagga
     4681 ggacattctt cgcaatcgcc tcctatcgga atctcccgtg accttagaag acttaggaag
     4741 aaaatatatg attaccaaag agcggactcg tcaactggag gctaaaataa ttaagaagtt
     4801 acgagagctt atgaaaaaag atattcagga ttttgaacat ctcagaaagt agcgctgtaa
     4861 gttcatatac gtacaaaaac cgtttacctc aagatttgga ggtgctttcc ttactaaaag
     4921 gatggccctc cgcctctcgt cattattgaa aatcttacct ttccctcttg acttcaattc
     4981 aaccagactt atctctccct ccaagttgac acatttttga aaaatttaaa caggtgtgcg
     5041 tcatcctaag ttagtactca ccattttctt aatcaattag caacaaccaa agaaggaggc
     5101 agacatggca gccacaaaag aaaaggaaaa gaaggaagcc aaagaaggaa gcagcgcgaa
     5161 ggggttccgt cccttagggg atcgtgtgtt tgtagcctat accgaagaat tggagcggac
     5221 agcaggggga atttacgtgc cggattcagc tcgggaaaaa ccacaaagag gcaccattga
     5281 agctgcagga cctgatgttg aaaatgtgaa agttggcgat caagtcttgt ttgacaagta
     5341 ttctgggacc aaaattaagg ttgaaaacga tgactgctta attttgaaag aagaagatat
     5401 tttgggcgtc ttcgaaaatt aacaccacca tcaagttcga actcactttt agtggcaatc
     5461 cacatcacca aaatatgttt ttaaaggagg atatgtatgg ctaagcagtt gcaatacagt
     5521 gaaaaggcac gtgcgtctat attgagcgga gtcaatcaat tggcagatgc ggtgaaagcg
     5581 acattagggc cgaaaggccg gaatgcgatt ctcgacaaga aatttggtgc cccaaccatc
     5641 accaaagacg gcgtgacggt tgccaaggag atcgagtta
//
LOCUS       DATIAL010000450         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1048237, whole genome shotgun sequence.
ACCESSION   DATIAL010000450 DATIAL010000000
VERSION     DATIAL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048237"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..223)
                     /locus_tag="VLA60_13915"
     CDS             complement(83..223)
                     /locus_tag="VLA60_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10504.1"
                     /translation="MNTIEWIVILGGLTAIAWVNWYFFMAQAMADKPIQIPAETGKSS
                     TR"
     gene            complement(220..438)
                     /locus_tag="VLA60_13920"
     CDS             complement(220..438)
                     /locus_tag="VLA60_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HSF10505.1"
                     /translation="MEDLTLTIEGMSCGHCVQGITTILSNLKGVKVDQVKIGEATLGF
                     YPQTITPDQIIHAIEEEGYKARLSMEAV"
     gene            complement(634..1230)
                     /locus_tag="VLA60_13925"
     CDS             complement(634..1230)
                     /locus_tag="VLA60_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HSF10506.1"
                     /translation="MKTEATSEMGKEAQHPPLLEEVMKQLTDHRQAFHAFLTRRLGNP
                     AFAEDLLQQCFMKALAHFHSIKKMDRIVPWFYQILRHALIDYYRSKETDSKRFQAFLL
                     ETETLGTNQVPSLDELQPTICACLDRLVFTLKPEYADIIRRIDLSGESLQSVAKDLHI
                     TTNNLTVRLHRARQALRKSLEDSCGICSKHGCLNCTCE"
     gene            complement(2039..>2657)
                     /gene="deoC"
                     /locus_tag="VLA60_13930"
     CDS             complement(2039..>2657)
                     /gene="deoC"
                     /locus_tag="VLA60_13930"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HSF10507.1"
                     /translation="TKDQIRQLCEEAVTFGFGAVCVAPLWVPFATRCLAGSPVRVATV
                     IGFPHGNTLPDVKAYEAKLAIEAGAHELDMVIQIGLLRAGNHGAVLDDIQGVVNVARR
                     ASRSKILVKVILETSLLNKEEQQIACQLVEKAGADYVKTSTGFTGHHVTIEEVGFLRS
                     LVGTRVGVKAAGGIRDLITAVALVNAGASRLGSSSSVSIMHMWPG"
BASE COUNT          596 a          684 c          750 g          627 t
ORIGIN      
        1 cccaaatgtc accagctggg cacagatcct tcgccaggag ctcaggatga caatgtcgat
       61 aggacgaggc atcatttccc gttcaccttg tggaggattt cccggtttct gcggggatct
      121 gaattggttt atccgccatg gcttgagcca tgaagaagta ccaattgacc caggcaattg
      181 ccgtcagtcc accgaggatg acgatccatt ctatggtgtt catacggcct ccatgctgag
      241 ccgggctttg tagccttcct cctcgatcgc gtgaattatt tggtcgggtg tgatggtctg
      301 cggataaaag ccaagggtgg cctccccaat tttcacctga tccaccttca cgcccttcaa
      361 attggaaagg atggtggtga taccttgcac acaatgcccg cagctcatgc cttcaatcgt
      421 gagtgtgaga tcttccatgg gaacctccgt ctgttgaaat tggtttgtgt gtgttgcatg
      481 tcctgttcgg tgtccgacat gcccgtttgc ttatatagac ggagctctct cctgattctt
      541 acagcgaggg attgtttaaa actggaacgg gaaaaaattg agcaaaaagg tggaagagga
      601 tgtgggagat gtagggagaa ggggggttgg ggcttattcg caggtgcaat tcaagcaccc
      661 atgcttactg caaatcccgc aggaatcttc cagggacttg cgcaaggcct ggcgcgctcg
      721 atgcaaacgg accgtgaggt tattggtggt gatgtgtaaa tccttggcga cggattgcag
      781 cgattctccg gacaaatcga tccggcgaat gatgtcggca tattcgggtt tgagggtgaa
      841 cacgaggcga tccaggcagg cacaaatcgt gggttgtagt tcatccagcg aagggacttg
      901 gttcgttccg agtgtctcgg tctccagaag aaaggcttga aaccgttttg aatccgtttc
      961 tttgcttcga taataatcaa tcagggcatg tcgaaggatt tggtagaacc aggggacgat
     1021 ccggtccatc tttttgatgg aatggaaatg ggccagggct ttcataaagc attgttgcag
     1081 gaggtcttcg gcaaaggcgg gattacccag acggcgggtc aggaaggcgt ggaaagcctg
     1141 gcgatgatcg gttagctgct tcatcacctc ttctaagagg ggagggtgtt gggcctcctt
     1201 ccccatttca gatgtggctt cggttttcat gtgctactca aggttgtcgt caacacgttc
     1261 aggagggcga gacgatagca cgcccggccg gcattcggga agatcgggta cgtgaaacga
     1321 aagagacgac ctggaaccat agcttcccgc ctacgagcgg cggaattgat gaatggaaag
     1381 ggcaaggaaa ggatacccgc tattttatgg tcatttcaaa taatttccga aatgtggctg
     1441 gttgggtgaa gcaccatcgt tcattgccgg ccaaccggtg agcgtccaca acaccacagc
     1501 acatcgtgaa atgcgatgtg gaattccctc agtctctcaa tccattcatt ccccttgacc
     1561 ggcgcctgcc gtccggtccc agaggcgaat gctttcatct acgggcgggc cctgtttgag
     1621 cctttcgaaa actcagggca ggctccgcga gttggcacgc cctcgaagga cctacatcgg
     1681 cacgctttaa ttttgatccg acgcctgccg gctggcccta gggagggacg ctcttcgcat
     1741 ttagcggacc ttgtttgagc agagcgagtt ggtccgctct tctaaaggtt ggcgtccctt
     1801 ccttcccaca aagccagacg gggcgcccat ggttttgggt ccttttgccg aaacaaaagg
     1861 acctcggctg ccggcccgaa acccggcatc tatcctacga ttcacatcat tttcagcaag
     1921 taagatgtgg gtggaagcag gcatgacttt gctccgaaca cagcggaagt ttattggaat
     1981 gactatctat ataataatgt cgaggagagg gagagagaat ttttaaggag ggcgagcttt
     2041 atcccggcca catgtgcatg atggacacgg atgatgaact ccccaggcgg gacgctccgg
     2101 cattgaccaa cgccacggca gtgatcaggt cgcggatgcc cccggccgcc ttaacgccca
     2161 cccgtgttcc gacaagactc cgcagaaacc ccacctcttc aatggtgaca tggtgaccgg
     2221 taaatccggt cgacgttttc acgtagtcgg ctccggcctt ctccacgagt tggcaggcta
     2281 tttgttgttc ttctttattt aagagcgatg tttcgagaat gaccttgacc aggatttttg
     2341 atcgggaggc acgacgcgcc acgttcacca caccctggat atcatccagg accgctccat
     2401 gattgcccgc ccgaagcaag ccaatctgga tgaccatatc caattcatgc gctccggctt
     2461 caatcgccag tttggcctcg taggctttca catccggaag cgtgttgcca tgaggaaagc
     2521 caataaccgt ggcaacccgg acgggagatc ccgccagaca ccgtgtggca aacggaaccc
     2581 agagcggggc gacgcagacc gccccgaagc cgaacgtcac cgcctcttca cagagctgtc
     2641 ggatctggtc tttggtg
//
LOCUS       DATIAL010000451         6866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1048394, whole genome shotgun sequence.
ACCESSION   DATIAL010000451 DATIAL010000000
VERSION     DATIAL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048394"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VLA60_13935"
     CDS             <1..263
                     /locus_tag="VLA60_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10508.1"
                     /translation="PNFAPAATAIGWFLYDKGQDDFAMDYFSRALKANPDDAQATFGV
                     GRIYEEKRKPELAVQHYQRALSLEKDPDKKMRILNFMDKLLG"
     gene            complement(322..600)
                     /locus_tag="VLA60_13940"
     CDS             complement(322..600)
                     /locus_tag="VLA60_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10509.1"
                     /translation="MGTQMGLRLEQRGDGNYYKVILHIGSTLIPISDDIVEELRNQTA
                     LPPDQFFALFVNKVGYSSYLQEQIRQEVRNTGDLNPQISVIQEFFRKE"
     gene            806..1888
                     /gene="serC"
                     /locus_tag="VLA60_13945"
     CDS             806..1888
                     /gene="serC"
                     /locus_tag="VLA60_13945"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HSF10510.1"
                     /translation="MARLYNFSAGPAILPEEVLKQAQAELPDWHGCGASIMEMSHRGK
                     EFVSVAAEAEQDVRDLLGVPANYKVLFLQGGASTQFATIPMNILRGKSKADYILTGAW
                     GKKAVSDAKKYCEVNVAASSESDNFTSIPPFEGWALSQDAAYVHYTPNETIGGVEFHW
                     VPDSGEVPLVADFSSTILSRPIDVSRFGIIYAGAQKNIGPAGLTLVIVRDDLIGNVLP
                     ITPSVYDYAKQAEADSMLNTPPTYALYIAGLVFKWLKKQGGLSAMSAINQRKASKLYA
                     AIDASKFYRNPVEKASRSWMNVPFVLANPDLDKAFLAGATEAGLTTLEGHRSVGGMRA
                     SIYNAMPEAGVDALIDFMADFEKRKA"
     gene            2056..3639
                     /gene="serA"
                     /locus_tag="VLA60_13950"
     CDS             2056..3639
                     /gene="serA"
                     /locus_tag="VLA60_13950"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HSF10511.1"
                     /translation="MNILVSDSLSPKGVEVLERAGFSVDVKTKLTKEELLQEIPKYEG
                     LVVRSATKVTKDVIEAGTRLRIVGRAGTGLDNVDSEAATRRGIVVMNTPGGNTITTAE
                     HTMSMIASMSRKIPQATMSMKAGKWEKTRFMGTELYNKTLGLVGLGQIGSYVAKLAQG
                     WSMNVIGYDPYLAVERAKQMGIEVVDLNELFHRSDVISVHTPLTNETRGLINTETIAK
                     MKDGVMIVNCARGGIINELDLCEALKSQKVAAAAFDVFENEPVDPKHPLMDLDNFICT
                     PHIGASTEEAQENVAIGIAEQFVDYFKRGIARGAVNVPSVAPEALPQLQPYLVLAERM
                     GRFQAQLLDGGIKSVTVEYFGEVAQLTVAPVTVAVLKGLLSPILEDVINYVNAPIVAK
                     ERGIEVKEVKSSDAGDFSSLIRIKVEAGSHTYSLGGTLFHRQEPRFVEINQFQVEVVS
                     EGQMILIDNVDRPGVIGMVGQILGQHDVNIARMQCARVERGASALLIIGLDAPLASAV
                     LDLLKKEKDILSVRMVDLS"
     gene            3711..5036
                     /gene="hisZ"
                     /locus_tag="VLA60_13955"
     CDS             3711..5036
                     /gene="hisZ"
                     /locus_tag="VLA60_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HSF10512.1"
                     /translation="MKPLRSLIPIGTATLLPHTAQSVRWLEDQLLAHCSTWGYQEIIL
                     PTFEYLDVLAVGLAPEILEKCYKFADWGSGRILVLRPDATAQIARMVAMGLGGDTLPL
                     RFSYRTTVFRYEPEHRGRDREVFQVGFELLGNDTSQSDAEVVTLLAGLLERIGLPKWK
                     ISLGHVGFFKALLAQSGLSLSGRKQAEIAAAHKDLPQLEKILETERLSRSKVKDILQV
                     PERYGRDEVLEWGMRIAGKDTQLLKPLQRLTQVYGQLIANGVQDQILLDLGEFRGFDY
                     YDGVVFDVFTSTFGSEIGGGGRYNHLVGRFGRDLSAIGLGLDLDRVFSALERGGTVGG
                     NGLKPVRIFTSPHQSEASFALAQRLRGAGICVIEDMIEGSPKSVLSWVTTVARRRGVP
                     CAMIFEEKTSTPQSVLLLEFTSYSQKPRQTRMLVQEVPQRLQAFSHGNI"
     gene            5023..6378
                     /gene="dnaB"
                     /locus_tag="VLA60_13960"
     CDS             5023..6378
                     /gene="dnaB"
                     /locus_tag="VLA60_13960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HSF10513.1"
                     /translation="MAISEAPEIRVPPQNLEAEQSVLGAILLDNAALNRAMEILSEDD
                     FYRTGNRIVYRGMVALSERNQPVDQITLTDYLRGTGELDQIGGASYIAEIVQVVPSAA
                     NIRYHSNIVRDKSLLRGLVQTATEVAMRGYEGTTGTNELLEFAEREIFRLAQGHLGGT
                     FAPVSSIIKDSVEIVDRLYSRKERITGVPTGFSDLDNMLAGLQPSDLIIVAGRPSMGK
                     TSLALGMVEYASLRSNAVVGIFSLEMSRAQLVLRMLSSQALLDSHALRTGQLTKHDWV
                     ALTEAASRLEQARIFIDDSGNLTLQQMRGKARRLKAEHGLDLLVVDYLQLMEGRGDSE
                     SRQQEISDISRALKGLAKELNIPVIALSQLSRAVENRKPPIPVLADLRESGAIEQDAD
                     VVMFIYREEVYEPDTEQKGIAQILVRKHRNGPIGEVDLQFHDRYAKFNNLTRERQAGI
                     V"
     gene            6400..>6866
                     /locus_tag="VLA60_13965"
     CDS             6400..>6866
                     /locus_tag="VLA60_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10514.1"
                     /translation="MNSTTVQVMAKKVRSGRTFPMWLGRLRTILWGIPLLVVSCGTAP
                     PPNLNGQEASMVTRETVHPQAYYHFLRGSLAELNNNAATALEEYQAGLAFDADSKFLK
                     FRLAKLHFSMSHPTEAVDLARQIPVSEISQAAMFFDLAKIFAGGGETGRALEI"
BASE COUNT         1695 a         1441 c         1873 g         1857 t
ORIGIN      
        1 aaccaaactt tgctccggcc gcgaccgcga tcggctggtt tttatatgat aaggggcaag
       61 atgattttgc catggattat tttagtcgag cgctcaaagc aaaccctgat gatgcccaag
      121 cgaccttcgg ggttggccgg atctatgaag aaaagcgcaa gccggaattg gcggtgcagc
      181 attatcagcg ggcactgtct ttagaaaaag acccggacaa gaagatgcgc attctcaatt
      241 ttatggataa attgcttgga tagatattaa atagcagtag ggggtggaca ggcatcagca
      301 ttcttcggaa gcggtgtaaa gttattcctt ccgaaagaac tcttgaatga ctgatatttg
      361 aggattgagg tctccggtgt ttcgtacttc ctggcgtatc tgctcttgta agtaggatga
      421 gtatcccacc ttgttgacga aaagcgcgaa aaattgatca ggtggaagcg cagtctggtt
      481 tcgcaattcc tcaacaatgt catcactgat ggggatcaaa gtactcccga tgtggagaat
      541 gactttataa taattcccgt ccccccgttg ttccaatctc agacccattt gtgttcccaa
      601 gttgttctcc aaccttcttg aaaatctatg aaagccttct ccaatcgccg gtcattgtag
      661 ggtatccaaa attggaataa caaggttccg tcctcttctt gactggtcta ggaccctatg
      721 ctagcgtaac ctctttttca cactggtgaa gttccgtaat tcaatatgta tgacgccggt
      781 ttaggaatgg aggacgagtc aaataatggc gcggttatat aactttagtg cgggtccggc
      841 gatattgccg gaagaggtgt taaagcaggc acaagccgaa ttgccggatt ggcatggctg
      901 tggcgcatca atcatggaaa tgagtcatcg tgggaaagag tttgtctcgg tggctgcaga
      961 agccgagcaa gatgtccgcg atttattggg ggtgcctgct aattataaag tgctcttttt
     1021 gcaaggtggg gcgtccacgc aatttgccac gattcccatg aatatcttac gtgggaaaag
     1081 taaggctgat tacattctga ctggtgcatg gggcaaaaaa gccgtaagcg atgcaaaaaa
     1141 atattgtgaa gtcaatgttg cggcatcttc cgaaagcgat aactttacga gtattccgcc
     1201 atttgaaggg tgggcattaa gtcaggatgc cgcctatgtg cattacacgc ctaatgaaac
     1261 gatcggtggc gtggagtttc actgggtccc agattccggc gaggtgccgt tagttgccga
     1321 tttttcatcc accattttat cgcgtcccat tgatgtcagt cggttcggaa tcatttatgc
     1381 tggcgcacaa aaaaatattg gcccagccgg tttgacatta gtgattgtgc gggacgacct
     1441 cattggaaac gtattgccga ttaccccaag cgtgtacgat tatgccaaac aggcagaagc
     1501 ggattctatg ttgaatacac ccccaacgta tgccctgtat attgcgggtt tggtgtttaa
     1561 atggttaaaa aaacaaggtg ggctttcggc gatgagtgcg atcaatcaac ggaaggccag
     1621 taaactctat gcagcaattg atgcgtctaa attttaccga aatcccgtgg aaaaggcctc
     1681 tcgttcctgg atgaacgtcc ccttcgtctt ggccaatcct gatttggata aggcatttct
     1741 tgcaggagcg acagaggccg gattaacaac acttgaaggc catcgttctg ttggcggaat
     1801 gcgcgccagt atttataacg cgatgcctga agctggcgtt gatgcgttga ttgactttat
     1861 ggcggacttt gagaaacgca aagcctaatg tctccccgga aatccggtta cggatgactc
     1921 tctaagatac aagataatgg tgcgaatatg tttgccacaa ccctgtgtgt gaatgagatc
     1981 cctcttaaaa tgccaagagt gctcaagatt ttcctgtgag aactaaagtg gggcctcccc
     2041 tatattggag atactatgaa tattttagtg agtgacagcc tttcccctaa aggagttgaa
     2101 gtcttagagc gggccggatt ttcggtggat gtgaaaacca agctcacgaa agaggaattg
     2161 cttcaagaaa ttcccaaata tgaaggttta gttgttcggt ctgcaaccaa agtcactaaa
     2221 gacgtgattg aggcaggcac tcgtcttcgt attgtgggcc gtgccggaac cggtcttgat
     2281 aatgttgaca gtgaggcggc aacccggcgc ggtattgtgg tcatgaatac tccaggtgga
     2341 aatactatta ccactgcgga gcacacaatg tcgatgattg cttcgatgag tcgaaagatt
     2401 ccacaggcga ccatgtccat gaaggcaggt aagtgggaaa aaacccggtt catgggaaca
     2461 gaactctata ataaaacttt ggggttggta gggttgggcc agattgggtc gtatgttgca
     2521 aaattggccc agggatggtc catgaatgtc attggctatg atccctattt ggccgtggag
     2581 cgggccaaac aaatggggat tgaagtggtg gatttaaatg aattgttcca tcgttctgat
     2641 gtgatatcgg ttcatacgcc cttgaccaat gaaacgagag gcctcattaa caccgagacc
     2701 atagcaaaga tgaaagatgg ggtcatgatc gtgaattgtg ccagaggcgg tatcataaat
     2761 gaactggatt tatgcgaagc gttgaaatcc cagaaagttg ccgcggcggc ctttgatgtg
     2821 tttgaaaatg agccagtgga tcccaagcat cccctcatgg atcttgataa ctttatctgc
     2881 accccccata taggtgcttc taccgaagaa gctcaagaga atgtggcaat tggaattgct
     2941 gaacaattcg ttgattattt caagcgaggc attgccagag gggctgtgaa cgttccctct
     3001 gtggcacctg aagcgcttcc ccaactgcag ccctatttgg tcttggcgga acgtatgggt
     3061 cgatttcagg ctcaattatt agacggaggg attaaatctg ttaccgttga atattttggt
     3121 gaagtggctc aacttaccgt ggcaccagtg accgtcgctg tgttaaaggg ccttctctct
     3181 cctatcttgg aagatgtcat caattacgtg aatgccccta tcgtggcaaa agaacgtggt
     3241 attgaagtta aggaagtgaa gagtagtgat gccggagact tctccagttt aatccggatt
     3301 aaagtcgaag ctggttctca tacctattcc ctgggtggaa ctctttttca tcgtcaagaa
     3361 ccgcgttttg tcgaaataaa ccaatttcag gtcgaggtgg tctcggaagg ccaaatgatt
     3421 ttgattgata acgtggatcg ccctggggtg attggtatgg tgggtcagat tttaggccag
     3481 catgatgtca acattgccag gatgcaatgc gccagagtcg agcgtggtgc atcagcactg
     3541 ctgattattg gtctggatgc cccgcttgct tccgccgtgt tggatcttct caagaaagag
     3601 aaagatattc tgtccgttcg catggttgat ctcagctagc ttgacggctt ctttctgcag
     3661 actatcttgg ccaaacattt cgagttggca ggttctcttc catacgcctc atgaaaccac
     3721 tccgctcgct tatccccatt ggcacagcta cattacttcc ccatacggcg caaagcgttc
     3781 ggtggttgga agatcaattg ttggctcatt gttcgacctg gggctatcag gaaatcattc
     3841 tccctacctt tgagtatctt gatgtattag ccgtgggctt ggctcctgaa atcctggaga
     3901 aatgctataa atttgcggat tggggatcag gcagaattct ggtcttgcga cctgatgcga
     3961 cggctcagat tgcccggatg gtggccatgg gccttggagg agatactctt cccttgcggt
     4021 tttcctatcg gaccacggtt tttcggtatg aaccggaaca tcgtggacgg gaccgtgaag
     4081 tatttcaggt cgggtttgaa ctgcttggca atgatacttc tcaaagtgat gccgaagtcg
     4141 tgactttatt ggcggggctt ctggaacgga ttgggttgcc aaaatggaaa atttctcttg
     4201 gtcatgtcgg attttttaaa gcattgttgg cccaatccgg tctgtctctt agtgggcgaa
     4261 agcaggcaga aatcgcggca gcccataaag accttcctca gctggaaaaa attttagaga
     4321 ctgaacggtt atctcgttct aaagtaaagg acatattgca ggtgcccgaa cggtacgggc
     4381 gggacgaagt gctggaatgg ggaatgcgaa tcgctgggaa ggatacgcaa ttgcttaagc
     4441 ccttacaacg tctaacgcag gtgtatgggc aattgatagc aaacggggtg caagatcaaa
     4501 ttcttttgga tcttggggaa tttcgtggtt ttgactatta cgatggtgtc gtgtttgatg
     4561 tctttacctc tacgttcggc agtgaaatcg gtggaggagg gcgctacaat catttggtcg
     4621 ggcggtttgg ccgggacctt tccgcgattg gattagggtt ggatttggat cgcgtgtttt
     4681 ccgccttgga gcggggagga acggtgggcg gcaatggatt gaagcctgtc agaattttta
     4741 cttctcctca tcaatctgag gcctcttttg cccttgctca acgattgcgg ggggctggga
     4801 tttgtgtgat tgaagatatg atagaaggtt ctccaaaatc ggttttgagc tgggtcacca
     4861 cagttgctcg gcgtcgaggg gtgccgtgtg caatgatttt tgaagaaaag acttccactc
     4921 cgcaatcggt gctcctatta gaattcacct cttattctca aaagccccgg caaacccgga
     4981 tgctagttca agaagttcct caacggttac aagcattcag ccatggcaat atctgaagcc
     5041 ccagagatcc gagttcctcc ccaaaatctc gaagcggaac aatccgttct tggagccatt
     5101 cttcttgata atgcggcgct taatcgagcg atggaaatct tgtcggagga tgacttttat
     5161 cgaacgggca accggatcgt gtatcgtggc atggtggcat tgtcggaacg gaatcaacct
     5221 gttgatcaaa ttaccctgac agactatttg cgagggaccg gcgaactcga ccagattggg
     5281 ggcgcttcgt atattgcaga aatcgtacag gtggttccca gtgcggccaa tatccggtat
     5341 cacagtaata ttgttcgaga taaatccttg cttcgtggcc ttgttcaaac tgccaccgag
     5401 gtggccatgc gaggttatga agggacgacg gggacgaatg aacttcttga atttgccgaa
     5461 cgggaaattt ttcgattggc tcaggggcat ttaggaggaa cgtttgctcc tgtcagtagt
     5521 attattaagg atagtgttga gattgttgac cggttgtata gccgaaagga acggattacc
     5581 ggagtgccca ccgggttttc ggacttggat aatatgctgg ctgggcttca accatctgat
     5641 ttaattattg tggcgggtag gccgagtatg gggaagacca gtttggcctt agggatggtc
     5701 gaatatgctt cacttcgatc caatgccgta gtcggaatct ttagtttaga aatgtctcgt
     5761 gcacagctgg ttttgcgtat gttgagttcc caggcgctgt tagactctca tgctctccgg
     5821 actgggcagt taacgaaaca tgattgggta gccttaactg aggcagcgag tcggttggag
     5881 caggccagga ttttcataga cgattctggg aatttgactc ttcagcaaat gcgagggaag
     5941 gctcgacggt tgaaagccga acatgggttg gacctcctgg tggtcgatta tttgcaattg
     6001 atggaggggc gtggagattc agaatctcgc caacaagaaa tttcggatat ttcccgggcc
     6061 ttgaaaggat tggcaaaaga gttaaatatt cctgtgattg ccctttctca gttgagccgg
     6121 gcggtggaaa atcgaaagcc gcccatcccc gtactggctg atttgcgcga gtcgggggcg
     6181 attgagcaag atgccgatgt ggtgatgttt atctatcgcg aggaggtcta tgagcctgat
     6241 actgagcaga aaggcattgc gcaaattctg gtgagaaaac atcggaatgg accgattgga
     6301 gaagtggatc tgcaatttca tgaccggtat gccaaattta ataacttaac gagagagaga
     6361 caggctggta tagtataatg agtcgttgag ggacggtata tgaattctac aactgtgcaa
     6421 gttatggcga agaaggttag gtcaggacgg acattcccca tgtggttggg tcgtctgaga
     6481 accattttgt ggggaattcc actactggtg gtgtcctgtg ggacagcccc ccctcccaat
     6541 ttgaatggac aggaagcgtc catggtgacg cgggaaactg tccaccctca ggcctactat
     6601 cacttccttc gaggttccct tgccgagttg aataacaatg ccgcaacagc tttagaggaa
     6661 tatcaagcag gtttggcatt tgacgcggat tcgaagtttt taaaatttcg cctcgccaaa
     6721 ctgcacttct caatgtcgca tccgactgag gccgtggatt tagcaaggca aatccctgtg
     6781 tcggaaattt ctcaggcggc aatgtttttc gatttggcga aaatttttgc cggtggaggg
     6841 gaaaccgggc gggccttgga gatttt
//
LOCUS       DATIAL010000452        14491 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_619292, whole genome shotgun sequence.
ACCESSION   DATIAL010000452 DATIAL010000000
VERSION     DATIAL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619292"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VLA60_13970"
     CDS             <1..659
                     /locus_tag="VLA60_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10515.1"
                     /translation="QTTVLKGSQLPEKQGWQDSSNAVRPLDVVTNGSHVTLNTIGVLR
                     AIDQLPGKAYMWFYKDLPFDFETGFSIEFTLQVHKVEQPHNFLDSGIMFYGSVDISDG
                     TFAEGPRSHMIYFDEDAIGWGDEKGMFKMDTTDTFHTYTLTVEGGRFAKVFVDGKPAL
                     KRNDWVGIPRIGFGDMTNDNGVNGKFSIGDIIVTSDPQRFPSRPAPRRQLIPPSRRGG
                     "
     gene            complement(846..1598)
                     /locus_tag="VLA60_13975"
     CDS             complement(846..1598)
                     /locus_tag="VLA60_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10516.1"
                     /translation="MGLLKKLRGMFNRRKTISTNPLYEIVLTHLQKDIHESPHEFIQK
                     FSEASKEKILQEIDNVTETLWQAPDRVLANREGLLESMLHQVDCEIFMIEPGHRLAGF
                     NGVSGELKDFLTEFAQKRIDTGELDWKQKTSPTKDEAYKLVWSKWLRANQYCKIFNAI
                     RLYLKDYHTNQERDWFFPLQCASAAFTEYNFRKEYGLIQIIDGARALQYGSFLEMVSK
                     GHKDPLDEWEKTYNELFPLHSSSYAESRNGKN"
     gene            1646..1810
                     /locus_tag="VLA60_13980"
     CDS             1646..1810
                     /locus_tag="VLA60_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10517.1"
                     /translation="MKKSTRHGIFSYLCLALTNLPAVCPPTSGFRQIGIDEKTVFLCQ
                     DRGNPIASEL"
     gene            complement(1960..2610)
                     /locus_tag="VLA60_13985"
     CDS             complement(1960..2610)
                     /locus_tag="VLA60_13985"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSF10518.1"
                     /translation="MTRKNDCWSTKGLLRVPVRLPNGEEFDVYNTDLDAGRDEEDRIV
                     RKKQIKFLAKCIKKLSKDRAFILGGDFNSQYDHGGIEASGEDYPILKHYLLDELALND
                     SNARPEPNGYWKNRVDYIFYRGGQGARIELIGSGSEDQRFRHPVNRGRQSPNEKYEDL
                     LSDHPAIRAEFKILPSPISAHTSRDGEIDSCAEEPVKEKMEIVWEDKLSSTFSARS"
     gene            complement(2607..2924)
                     /locus_tag="VLA60_13990"
     CDS             complement(2607..2924)
                     /locus_tag="VLA60_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10519.1"
                     /translation="MRNDDTRSGLMAWLLMDYDFVLLQENFEFHRQIVHEFTYELIEQ
                     GNGPRIALAPLWLVTLPIKIANVGFQLFNSPGIPFRVPYGSGLTIASPYYNRYRFQIM
                     KDG"
     gene            3325..3486
                     /locus_tag="VLA60_13995"
     CDS             3325..3486
                     /locus_tag="VLA60_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10520.1"
                     /translation="MAICTRRHHSPAFKVEAVLAIIRQDCTLAELTEQFGAPEGFFRH
                     RTRIRSMTE"
     gene            complement(3779..4099)
                     /locus_tag="VLA60_14000"
     CDS             complement(3779..4099)
                     /locus_tag="VLA60_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10521.1"
                     /translation="MRALIFFGCFLGMAGNTWAQIGEDPWRPASQLHPTPPRSFYDQT
                     PIPSSPGSVQNFDAWYQQQRVQQRQQHDRYRQEALQHDLNQMNRLRTYDRLRWGPLQP
                     YSPY"
     gene            4780..5097
                     /locus_tag="VLA60_14005"
     CDS             4780..5097
                     /locus_tag="VLA60_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10522.1"
                     /translation="MKPFANGVRIFALPMQASSRNDKIVLVILGNAITCMILQGQQHY
                     LWRTVAQDDERIDMRVQLRRIHMQSVNNLLLRSQAFQVWKESVCAKGVRENPFQCDLI
                     KFN"
     gene            5129..6019
                     /locus_tag="VLA60_14010"
     CDS             5129..6019
                     /locus_tag="VLA60_14010"
                     /inference="COORDINATES: protein motif:HMM:NF015457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10523.1"
                     /translation="MASTTGMRGGGFYDAHSSGQRAAMDEFLPWIVDAMADLPLPTSG
                     SPIALLDLGSSEGANAIHAVGAIIEALRERTSADIWTFFDDLPTNDFNQLLANLFPNA
                     TLSSRTAGIYPAAVGGSLFGRVAPAASLDIATTFNAVGWMSVLPPTRLPRFISPSPPA
                     QRAFREGVSVSPEERAAFTKQAHEDILSFYRARAAELKPGGKLLVQVFGRNDEHDTAN
                     GIIDGLSGAMLDMVEEGRLTRDVYENFIFPVYYRSLSELLPPTGESGEPPAFHVDKAE
                     ARDCRVPFNEEYARSGDPRA"
     gene            6065..6217
                     /locus_tag="VLA60_14015"
     CDS             6065..6217
                     /locus_tag="VLA60_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10524.1"
                     /translation="MTSGLPNTPDRPQLIDELYARMTKRFAADPERYGFHFISLGVLP
                     TRVLSI"
     gene            complement(7092..7180)
                     /locus_tag="VLA60_14020"
     tRNA            complement(7092..7180)
                     /locus_tag="VLA60_14020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7144..7146),aa:Ser,seq:cga)
     gene            complement(7195..7683)
                     /gene="tadA"
                     /locus_tag="VLA60_14025"
     CDS             complement(7195..7683)
                     /gene="tadA"
                     /locus_tag="VLA60_14025"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HSF10525.1"
                     /translation="MEVLDPTTDSQFMRMALDLAHQAFRAEEVPVGAVLVQNKKILAT
                     GFNQRESTQDPTAHAELMAIRSASMTLGSWRLTDTTLYVTLEPCAMCAGAIIQARIAR
                     VVFGTWDPKAGACGSICNLTAETRFNHRVKVDSDILADDCRAILQNFFQHLRSNQPVP
                     TA"
     gene            7864..8577
                     /locus_tag="VLA60_14030"
     CDS             7864..8577
                     /locus_tag="VLA60_14030"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HSF10526.1"
                     /translation="MWQSLAHHFFENRLPLITIEWSTRLTASAGLFVSQIGPRSWWAS
                     RDYRQGAARVIRLSAPLLRDQPEEELRRTLAHEMIHQWQFDIRKHRPSHGAEFREMMD
                     RMNAAGLGVTVRHQLNVAVQRHHRYAWQCLQCGMAYHRQRRTIIPARHICSRCRGKLV
                     EVELHHTQKTGEESMDGGGVNRGGHQAEDESAVRMLCVGGMKEAEALSTKGVFTPDDF
                     REEFISEFGGPAMAPPTND"
     gene            8815..9819
                     /locus_tag="VLA60_14035"
     CDS             8815..9819
                     /locus_tag="VLA60_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HSF10527.1"
                     /translation="MDILILFASLVVILLGAEAFTNSLEHFGERLGISEGVTGSVFAA
                     VGTALPETMVPIFAILLSSGSETLRHEVGVGAILGAPLMLATLAFFLMGFFAARKRGW
                     HGTLNPEPIGFARDLLWFNWAFCLGVIAVFVPQEWGWVRPAIAGALVILYVVYLYQTI
                     RSSANLVDDGHGTEADHPLYITKVGLPDNLMVILFQVGCGLGLIVLGAKGFVYGIEHL
                     APKWGVSALTLSLLIIPIATELPEKVNSIIWIRRDRDTLAVGNITGAMVFQGSLLPAL
                     GIMLTAWVPQHEILMGMAMTLVAACYLTWVVRRGALKPIHLVMNGLCYVVFVVSVLFW
                     "
     gene            10077..11156
                     /locus_tag="VLA60_14040"
     CDS             10077..11156
                     /locus_tag="VLA60_14040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HSF10528.1"
                     /translation="MALPIVVVTRRLPQQAWEVLGAQADLVCWEYDCPVDRKWLLDHL
                     PEAEGLYCLLTDRIDREVLQVGKRLRVISTMAVGYDHIDVEACTTRGIPVGHTPGILT
                     ETTADLAFALLLSAARRIVEGVEYVREGQWTTWSPDLLLGQDVHGATLGIVGFGRIGQ
                     AMARRAQGFQMDVLAVPSPKGGNGAPQSSVSLGPGTQPIAKPTMHLKGSFEGTSQGES
                     GGAAFHEVDLHEALALADFVSLHVPLTPHTHHLIGEAEFRAMKPSSILINTARGGVVD
                     SEALYHALVHGHIGGAALDVTDPEPMPVTHPLLTLPNCLVIPHLGSASVATRTRMACM
                     AAENIVAGLRGDALPHCVNPEVYRV"
     gene            11294..11605
                     /locus_tag="VLA60_14045"
     CDS             11294..11605
                     /locus_tag="VLA60_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HSF10529.1"
                     /translation="MIHITPPAVEKLKALIEEHPEDPIVRLAVRDRDDKVLVFSITLE
                     DAVTQEDSVLDIEGLIIAIDGSCAQRLEGVTVDYEEAAGFSFHHPEPPDPYKLNLILP
                     E"
     gene            11715..12902
                     /locus_tag="VLA60_14050"
     CDS             11715..12902
                     /locus_tag="VLA60_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HSF10530.1"
                     /translation="MMELFFATCPRGLEEVLSQELQELGGQEIRSTPGGVECQGAFPL
                     CYRLNLESRIASRILWRVGQGWYRNEEDLYRLASRLPWPEYFSVDCHIKVRIIAQHSP
                     LKSLEFATLRVKDAICDRFVRATHARPEVSKRQPDIQIVVFVDAEHVVWYIDTSGDPL
                     FKRGWRKVAGDAPIRENLAAGILRLSGWTGEQVLLDPMCGAGTFLIEAALMAKGIAPG
                     RGREFAFRHLQNFDDTIWDHVRQESIKSIPPRPGLSIVGYDEDANALTMARSNLEGLG
                     LEDIQLGQVDVLDVMPPASKGFLVTNPPYGVRMGDRSELEEWYPKFGNLLKQRFAGWD
                     VYVLTADSRLPKLIHLAPSRKIPLFNGPLESRLYEFQMVAGGNRKSARQARQQAVNRP
                     GLR"
     gene            complement(12895..13347)
                     /locus_tag="VLA60_14055"
     CDS             complement(12895..13347)
                     /locus_tag="VLA60_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10531.1"
                     /translation="MRGAVKNTFKILTIEDDIAQIEALKEALTVAQIICVEIHEAEDG
                     EQAMAFLNQEYPFMDAPQPDLIFLSNHLPDVSGEEIIKRLQRDRRLQSIPVALFSEET
                     PRPSKDFSFPPNCHLFKRPKTCDEWIYVLRCIEDVWVSLLNMAGKPLI"
     gene            complement(13717..>14491)
                     /locus_tag="VLA60_14060"
     CDS             complement(13717..>14491)
                     /locus_tag="VLA60_14060"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HSF10532.1"
                     /translation="QELSIIRQALVRASDRLHRIQYEGLAVQIKPDGSPVTNADLEVN
                     RIVQEALFAVYPDDGWLSEESPDNPIRLKKNRVWILDPIDGTKPFMKYLPQFAISLAL
                     IDHGQASIGIIFNPATREYYCAVRGEQATVNGCPIHVGRTTGQRLSFLVNTGPIDRST
                     IRTWRETANCRNLMGSIAYSLALVAAGQIDGVINIGTQNEWDIAAALLLVQAAGGVVL
                     DRDLKSIQCNQPHPIVNGIIAARPDAIPVIQQLLGSLPG"
BASE COUNT         3430 a         3399 c         3964 g         3698 t
ORIGIN      
        1 gacaaactac cgtattgaaa ggttcccagt tgccagaaaa gcaaggttgg caggattcca
       61 gcaatgctgt tcgcccattg gatgtggtga caaacgggag tcatgtcacg ctcaatacaa
      121 ttggagtcct aagagccatc gatcaacttc cagggaaagc gtacatgtgg ttttataaag
      181 accttccttt tgacttcgaa acagggttct ccattgagtt tacattgcaa gtgcacaaag
      241 tggagcaacc gcataatttt cttgattcgg gcattatgtt ttacggttct gtcgacatct
      301 ctgacgggac cttcgcagaa ggacctcgaa gtcatatgat ctattttgat gaggatgcca
      361 ttggatgggg ggacgagaaa gggatgttta aaatggatac gactgatacc tttcacacct
      421 acacgttaac ggttgagggt ggaagatttg ccaaggtgtt cgttgatgga aagcctgctt
      481 tgaaacgaaa tgactgggtg ggtataccca gaatcggctt tggagatatg acgaatgata
      541 atggggtcaa tggaaagttc tctataggcg acataattgt gacctccgat ccccaacgct
      601 tcccttcgcg acctgccccc cgccgtcaac tcatccctcc ttcacgacgt ggtggttaat
      661 ccacctatgg ataaaccaaa cacatcgaga ctcaccgtct gaaaaatttt gtaagggatt
      721 tcgttattca tcagagtaat atgaggcaga ttttggagga accgacaaga gggagcagat
      781 gaaggtggat ggagggatat tggtttgctg gtgactcgtt ttttagctga aggaaaaata
      841 ttggcctagt ttttcccatt cctggactca gcataagacg aagaatgtag agggaataat
      901 tcattatagg tcttttccca ttcatctaag gggtctttgt ggcctttaga aaccatctca
      961 agaaaggaac catattgtag ggctcgagca ccatcaatga tctgtatgag gccatattct
     1021 ttgcgaaagt tatattctgt aaaagctgcc gaggcacatt gaaggggaaa gaaccagtct
     1081 cgctcctgat tcgtatgata atccttcaaa taaagccgta ttgcattaaa tattttgcaa
     1141 tattgattgg ccctcaacca tttgctccac accaacttat aggcctcatc tttggtaggg
     1201 ctggtttttt gcttccagtc taattcgccc gtgtctatgc gtttttgggc aaattcagtc
     1261 aggaagtctt tcaattcgcc ggaaacacca ttgaaacccg ctagcctatg gccaggctca
     1321 atcatgaata tttcacaatc cacctggtgc aacatggact caagcaaccc ttcacggtta
     1381 gccaatactc tatcgggagc ctgccatagg gtttctgtca cattgtcaat ttcctgaagg
     1441 atcttttcct tcgatgcttc agaaaatttt tgaatgaact catggggtga ttcatgaatg
     1501 tctttttgta aatgggtcag aacaatttca taaaggggat tggtggatat ggtttttctg
     1561 cgattgaaca taccccgtag tttttttaac aatcccatac aaaacctcca caagtaaggg
     1621 gtaggttttc cacaccggga gattcatgaa gaagagtact cggcatggaa tcttttccta
     1681 tctctgcctt gcactcacga atttacctgc tgtctgccca ccaacatccg gtttccggca
     1741 gattgggatt gatgaaaaaa ccgtctttct gtgccaggac cgaggcaatc caatagcaag
     1801 tgagctgtaa acaggcctta acagctatac aatttaatat agccaggatt tttcctattg
     1861 gccagagaat gaccgtagcc ggataaaggc agcaggaagg ttcactctag atgtctttgg
     1921 gcaaattggc gagatggggt atagtgattc ccgcagagat tatgaacgag ctgaaaaagt
     1981 cgacgacagc ttgtcttccc aaactatctc catcttctcc ttcacaggct cttctgcaca
     2041 cgaatctatt tccccatccc gtgaagtatg agcggagatt ggcgacggta agattttgaa
     2101 ttccgcgcga atggcagggt gatcgctaag aagatcctcg tacttttcat taggagattg
     2161 tctgcctcgg ttaacaggat gccggaaacg ctgatcttcc gatccagagc cgattaactc
     2221 aatcctcgct ccttgacctc ctcgatagaa aatatagtcc actcgatttt tccaatatcc
     2281 attgggttct ggacgtgcat tgctgtcatt gagagccaat tcatcaagga gatagtgttt
     2341 taaaattggg tagtcttctc ctgaggcctc aattccccca tggtcatatt gtgagttgaa
     2401 atcacccccc agaatgaagg cccgatcttt cgataatttc ttgatacatt ttgcaaggaa
     2461 ttttatctgc ttttttcgaa caattcggtc ttcctcatct cttccggcat ccaggtccgt
     2521 attgtatacg tcaaattctt cgccattcgg aagacgaacc ggcacgcgaa gaagtccttt
     2581 ggtcgaccag caatcatttt tcctcgtcaa ccatccttca taatctggaa gcggtacctg
     2641 ttatagtagg gtgaagctat ggtcagcccc gaaccatagg ggacacgaaa aggaatgccg
     2701 ggactgttaa ataactgaaa accaacattg gcgattttaa tgggaagggt gaccagccat
     2761 aatggggcca gagctatccg tggcccgttt ccctgttcta tgagctcata ggtgaattca
     2821 tggacaattt gccggtgaaa ttcaaaattc tcttgtaata atacaaaatc ataatccatg
     2881 agcaaccagg ccatcagacc acttcgggta tcatcattcc tcacaggtat tccgacatac
     2941 atggaattca gaatcggaga aattccgtga acattgtaag acagaaaccg aatggtacag
     3001 ggcacaggta ccaatgattc cgattgatcc gcagaacata cttcgcgtgg cgtaacacct
     3061 tctgctattg aaaactcgta accggaatct attaatatca tgctaagtaa gaagaccccc
     3121 ttcatcaagc attttgggat ggccatattt cacaactcct tttctattcc ggaactcgta
     3181 ttcataacga cgaccccatc agaggaacta gggtccccaa ctcccacaat taaaccgccc
     3241 cctactttac ttacctcaga tttaaaatca agtgttcctc acataatctt ctgagctatg
     3301 ttttttcaat ggaaggagca gaagatggca atatgcacac gacggcatca ctctcccgct
     3361 ttcaaggtgg aagcggtctt ggccattatc cgccaggact gcacattggc tgaattaacg
     3421 gaacagtttg gtgcaccaga aggatttttt agacaccgca ctcggatccg gtcgatgact
     3481 gagtgaaaag ccatgatgaa ggtcacggca gagctgtcgg tgatccgcca atgtgatgtg
     3541 ttggctgtac cacatggacg gcaaagggcg atgggtcgac aaggtgttgg tcgcacggct
     3601 ttggcgtagc gtgaagtacg aggatgtcta cttacgagcg catgagcttt cttcagcggt
     3661 atgattggga ctcacgcact actttcacct taaaactgta gcaagaagat gttagcccca
     3721 atcgggtttt acaggtaggg gaggaaataa tgttgcgaaa gagtccgtcc gccacagctt
     3781 aatatggtga atagggttgc aggggtcccc aacgcagtcg atcataggtc cgcagccggt
     3841 tcatttggtt taagtcatgt tgtagagcct cctgccggta acgatcgtgt tgttgccgtt
     3901 gttggacacg ctgttgttgg taccacgcat cgaagttttg caccgacccg ggggaagaag
     3961 ggataggggt ttggtcatag aaagatcttg ggggagtcgg gtgaagctgt gaagccggtc
     4021 gccagggatc ttcgccaatt tgggcccagg tatttcctgc catacctagg aagcatccaa
     4081 agaatattaa tgctcgcatg cggtagccct cctgtaaatg gggaaaaaga tgttcccaca
     4141 tggccgatca tttgtatcag ggggtagcgt ggcaagattc gcaaataaag gcaattcatc
     4201 aaataggtaa tgaaaggaaa gtttctgaca ccttcgcccg ttgctgttcc cgctgtggct
     4261 cgttggaggt cttttcccgg gtggcaacct cctccagccc atcctctggc atggccgata
     4321 attactgccc gaccatagaa gttttcttat caagccccag ttcagaaagt gcgagagtgc
     4381 cgttctcaat tagattggtt gacggagagc gagggaattt gaagtcgtgc gggcccggct
     4441 catcccgcat tcccacgagg gggccatgtc cccctcgtac cttcgcatag ccggggtcac
     4501 cccatgagag gggaaatatt gctattgcca aagcctactt ggcgaacacg ctccccgaac
     4561 cggtcttgat gtcgtgttca aattcaaggg ctcttccaga tattatcaat ttaacgcatg
     4621 tttggcacac tcgggggcat gaactccaaa acgcctggct atcaatgcca tcgtttccga
     4681 ccgaatttat tcgtcatgtg atgtgggtgt agtatcaatt ctgtttgagg tttcgggaag
     4741 tggaggagcc tctggttaaa cgatgcttta tcgccagcta tgaaaccgtt cgccaatggt
     4801 gtcagaattt ttgccctgcc tatgcaggca agctcaagaa acgacaaaat cgttctggtg
     4861 atacttggca acgcgataac atgtatgatc cttcaggggc aacagcacta tttgtggcga
     4921 accgtggccc aagacgatga gaggattgat atgcgtgtgc aactccgacg cattcacatg
     4981 cagtctgtca ataatctttt actgcgaagc caggcctttc aggtctggaa ggaatcggtg
     5041 tgtgcaaaag gggtgagaga aaatccgttt caatgtgatc tcattaagtt caattaacaa
     5101 tgccccgcga tgggcaggag acctgagcat ggcaagcaca actggtatga gagggggagg
     5161 cttctacgat gcgcactcat cggggcagcg cgcggcgatg gacgaattcc ttccctggat
     5221 cgtagacgcc atggccgatc tgcccttacc gacgagcggc tcgccgatcg cgctgcttga
     5281 cctcggctcg tcggaagggg ccaacgcgat tcatgcggta ggggcgatca tcgaggcgct
     5341 gcgcgaacgc acctcggccg acatttggac ttttttcgat gatctgccga cgaacgactt
     5401 caatcaactc ttggccaacc tctttcccaa tgcgacgctc tcgagtcgca ctgccggtat
     5461 ctatcccgct gccgtcggcg ggtcgctctt tgggcgggtc gctccggctg cgagccttga
     5521 catcgcgacg accttcaatg cggtgggctg gatgtcggtg ttgcccccga cgcgcctgcc
     5581 gcggttcatc tccccatcgc caccggcgca gcgtgcattt cgcgagggtg tctccgtgag
     5641 cccggaagaa agagcggctt tcacgaaaca agcccatgaa gacatcctga gtttctaccg
     5701 ggctcgtgcc gccgagctga agcccggcgg aaaactactc gttcaagtct tcggacgcaa
     5761 cgacgaacac gacaccgcaa atggaattat tgacggtcta agcggcgcga tgctggacat
     5821 ggtggaggag ggtcgcctga cgcgcgacgt ctatgagaat ttcatctttc ccgtctatta
     5881 tcgatcgctt tcggagttgt tgccgccgac cggagagagc ggggagccgc cggcgttcca
     5941 cgtcgataag gcggaggctc gcgactgccg agtccccttc aacgaggagt acgcccgcag
     6001 tggagacccg cgggcctagg ccgaaagcta tgccggattc attcgcgcgt tctcggaacc
     6061 cgttgtgacg tccggcttgc cgaacacccc ggatcggcca cagctgatcg acgagcttta
     6121 cgctcgaatg acgaagcgat tcgcggccga ccctgaacgc tacgggtttc atttcatttc
     6181 tctgggggta ttgccgacgc gggtattgtc aatttaacgc gggtttggct gactgcgctg
     6241 aatgaaaaaa ccaacagtcg gtcaccaacg acaccgtttt ctccagaaaa catggttgtg
     6301 gtgagttcga tccggccacg ggctctctcg gtctcataca caaaggcgca gtgggaccgc
     6361 taaaaaaccg ccagagaaat ctgcatgaaa aagagaaaaa tcagagggcg atgatcgggg
     6421 gagagtgtct cgatcttggc tccaagggag cctatctcgc ggacgagtta ggagtatttc
     6481 cgaaatgcgg ggaggagctg atgttgctgc aggctgcttg gcggagaggg tgggaatttg
     6541 aagtcgtgcg ggcctgctag ccccgcactc ccactagggg gccagccccc tcatgccttc
     6601 gcatggcttc ggtcactcca tgaaggggaa atgtccctct taccaaagcc ttttcctggc
     6661 aaatgcacga ccagagcccg tcttcaggta gcattcgaac tcaagggcct ttgagacatc
     6721 agaaaatccc atataggtta cgagtttcca aggtatgaat ttcgaagtat gaatagagcc
     6781 accgttgttg tgaacgatca gtcgtttttt tttagtcgga agtaatacca atgtaacttt
     6841 gtttagaatt tgattcgctt ctgatgagat agacatactc catgatagag gaatcatggg
     6901 agtccgcctt cgtcactacg tcgatcttgc tactcatgaa ccctatttct aaagtgaaag
     6961 tgagctcagc tatcgattgt gggctgcaag acttcggcgg acagccttcg cgtcatttcc
     7021 tgtgctggcc tgccagccga agctttagcc gcttgcgcgc caaagctggc tacagcggcg
     7081 agcgcgaagg ctggcggaga gggtgggatt tgaacccacg gtcccttgtg aggacaacgg
     7141 ttttcgagac cgtccgaatc ggccactctc gcacctctcc gtgttcgtcc tatgttatgc
     7201 ggtaggaact ggttggttac tccgtaggtg ctgaaaaaaa ttttgtaaga tcgctcgaca
     7261 atcatctgcc aggatatctg agtctacctt tacccggtga ttaaacctgg tctctgctgt
     7321 gagattgcag attgacccac aggcgccggc cttcggatcc catgtgccaa acacgactcg
     7381 ggcaattcta gcctgaatga tggctcctgc acacatggcg cagggttcca atgtcacata
     7441 gagggtggtg tctgtcagac gccaggatcc cagtgtcatc gaagcggatc gaatggccat
     7501 gagttcggca tgggcggtcg gatcttgtgt gctctcccgt tgattgaagc ccgttgcgag
     7561 gattttttta ttctggacca gcaccgctcc aaccgggact tcttccgcac gaaaggcctg
     7621 atgggccaag tccagcgcca tgcgcatgaa ttgactatcg gtggtgggat ccaatacctc
     7681 catggtcata tcaaaaagta cagttgaatg atgatggtgt gagtgaaagt ttttgttccg
     7741 aaaggcaaaa aaatggtaac atagccacta tctgatgtat agccaaccca cctggaaatc
     7801 ttggtgaaat cttccgccgg tatcgtgtgt cagaggctcg ttcctttgga aaaggtgcaa
     7861 gttatgtggc agagtcttgc ccaccacttt tttgagaatc ggttacctct gattaccatt
     7921 gagtggagca cacgattaac cgcatcggcc gggctgtttg tgagtcagat tggcccaaga
     7981 agctggtggg cctcacgtga ttatcgacaa ggtgcggctc gagtgattcg gctgtccgcc
     8041 cccttgcttc gcgatcaacc cgaagaagag ctccgacgaa cgttggcgca tgaaatgatt
     8101 catcaatggc agtttgatat tcggaagcat cgcccgtccc atggggcaga atttcgagaa
     8161 atgatggacc ggatgaatgc ggcgggactt ggggtgaccg ttcgccatca gttgaatgtg
     8221 gctgttcaac gacatcatcg ttatgcctgg caatgccttc aatgcggaat ggcctatcat
     8281 cggcaacgac gaacgattat tccggctcga catatttgta gccgatgtcg aggaaaactt
     8341 gtcgaagttg agttacatca cactcaaaaa accggtgagg aatcgatgga cggaggaggc
     8401 gtgaatcggg gaggacatca ggcagaggat gaatcggctg tgcggatgtt gtgtgtgggt
     8461 ggaatgaagg aggctgaagc cttatcgacg aaaggcgtct ttaccccgga tgacttccgc
     8521 gaggagttta tcagcgagtt tggcggcccg gcgatggcgc caccaacgaa cgattagagc
     8581 atagatcaca atggtactta ggacgagcaa gaagcctgtt gtgatgttgt ccatagaggg
     8641 agtgtagaag aataaatttc cagatggcaa ggtgtggggg cgtcctggga acggtgggtg
     8701 gtacctgaag aaaaatagcg ttaccttagt gcacgtaatt atatgaaagt attatgaaat
     8761 ttagcatgct ccttggaatt ttctattaat aaaaaggaaa cgtacctttt ccatatggac
     8821 atcctgattc tcttcgcatc tttggtcgtc attttgttgg gggccgaggc gtttaccaac
     8881 agtttagaac attttggaga gcgtctcggg atttcggaag gtgttaccgg ttcggtattt
     8941 gcggcggtgg gaacggcatt accggaaacg atggtgccca tctttgccat cttgctgtca
     9001 tcgggttctg aaacgttgcg gcacgaagtg ggggtgggag ctattctcgg ggcgcccctg
     9061 atgctggcca cattagcctt ctttctgatg gggttctttg ccgcacggaa aagagggtgg
     9121 catggcacgt tgaatcctga gccaatcgga ttcgcccgtg atctgctctg gtttaactgg
     9181 gcgttttgtc tgggagtgat tgcggtgttt gttccccaag agtggggttg ggtgcggcct
     9241 gccattgcgg gcgcgctggt tatcctgtat gtggtgtatt tgtatcaaac cattcgatct
     9301 tcagccaatt tagtggatga tggccatggg accgaggcgg atcatccgct gtatataacc
     9361 aaagttgggc ttcccgataa tttaatggtg atcttgttcc aagtgggatg cggcctgggc
     9421 ctcattgtgt taggggcgaa aggttttgtg tatgggattg aacatcttgc ccccaagtgg
     9481 ggggtttcgg ccttaaccct ctctctcctc attattccga ttgcgacaga attgccggaa
     9541 aaagtgaaca gcattatttg gatccggcgc gatcgggata ccctggctgt cgggaatatt
     9601 accggggcca tggtctttca ggggagttta ttgccggcct tgggaattat gctgacagcc
     9661 tgggtgccgc aacatgaaat tctcatggga atggcgatga cgttggtggc ggcctgctat
     9721 ttgacctggg tggtacggcg gggcgcgttg aagcccattc acctggttat gaacggtctg
     9781 tgttatgtgg tgtttgtggt ttcggtgctc ttctggtagg gagtgttgaa aaatgccgcc
     9841 atcggcgttc tcgccgcttg gccgtgctca cgtactcctc cgtacgctcc gctcgtccaa
     9901 gcggctgcgg ccttgctgga cgagcatttt tgaacactcc actatgtttc tctggtgagc
     9961 cttggtaact cataggctga ggaaacgaca aaattattgc aaaactcaac agcccttgct
    10021 aatccccgga ctttaatttt ttcacgagcc atgaagaaga aaaccaaaga ctaataatgg
    10081 cccttccaat agttgttgtc acgcgacgtc tcccacaaca ggcttgggag gtattagggg
    10141 cacaggccga tcttgtgtgc tgggaatatg attgccccgt tgatcggaaa tggttgttgg
    10201 atcatcttcc cgaggcagag ggtctgtatt gcctgttgac ggaccggatt gatcgggagg
    10261 tgcttcaggt tgggaaacgg cttcgggtga tcagcaccat ggctgtcgga tatgaccata
    10321 tcgatgttga agcctgtaca acccgtggca ttccggttgg gcacacgccg ggtattttaa
    10381 ccgaaacaac ggcggatctg gcttttgcct tgctcctctc tgcagccaga cgaatcgtcg
    10441 aaggggttga atatgtgcga gagggacagt ggacaacctg gagccctgat ctcttgctgg
    10501 gacaggatgt gcatggtgcg acgttaggca ttgtgggatt tggccggatc gggcaggcca
    10561 tggccaggcg cgcgcaagga ttccaaatgg atgtgctagc ggtgccgtcg ccgaagggtg
    10621 ggaatggagc acctcagtct tcggtctcac tggggccagg cactcagccc attgctaaac
    10681 caacaatgca tctgaagggc tcttttgaag ggaccagtca gggcgaatcg ggaggggcgg
    10741 cattccatga ggttgatctg catgaagcat tagccctggc ggattttgtg agtctgcatg
    10801 tccccctgac tcctcacacg catcatttga ttggggaagc cgagtttcga gccatgaagc
    10861 catcaagtat tctaatcaat acggctcgtg gcggcgtggt tgattcagag gccctgtatc
    10921 acgccttagt gcatggccat attggtgggg cagccttgga tgtgactgat ccggagccca
    10981 tgcctgtgac acacccgctc cttacgcttc ccaactgtct ggttatccca cacttgggga
    11041 gtgcgtctgt ggcgacaagg accagaatgg cctgcatggc cgctgagaat atcgttgccg
    11101 ggttacgcgg ggacgcgctg ccacactgtg tcaatcctga agtctaccgc gtgtaacgca
    11161 gttccgggac cgggatggtt cgaatcctta gggaaattgc tagatgactg tttacgctgg
    11221 aaaaggagtt cgatgatatg ggaaacatta caggtcaatc tgacgattgg cggtttcgtc
    11281 atgaggagag atcatgattc acatcacacc accggctgtg gaaaaattga aggctttgat
    11341 cgaagagcac cccgaggatc ccattgtgcg tttagccgtt cgggaccggg atgacaaagt
    11401 ccttgttttt tccattactc ttgaagatgc cgtgacacag gaggattcgg tcttagatat
    11461 tgaagggctc atcattgcca tagacggctc ctgtgcacaa cgtctggagg gcgtcacggt
    11521 tgattatgag gaggctgctg gattcagctt ccaccacccg gaaccccccg atccttataa
    11581 attaaacctg atcctacctg aatgaggcag acagaatggg attggggcat ccactgaacc
    11641 cgatggcggg aatattcttc atcaatcatt cttgctaagc ttacccggta accatatatc
    11701 ccgcgattat tcttatgatg gaattatttt ttgcaacctg tcctcgtggc ttagaagaag
    11761 ttctgagcca agaacttcaa gaattaggcg gtcaggagat tcgatcgact cctggcggcg
    11821 ttgaatgtca gggtgctttt cctctctgct accggctcaa cttagaaagt cggattgcca
    11881 gccgtatttt gtggcgggtc gggcaggggt ggtaccggaa tgaggaggac ctgtatcgcc
    11941 tcgcctcacg tcttccctgg ccggaatatt tttccgtgga ctgtcacata aaagttcgca
    12001 tcattgccca acattctcca ctgaagagtc tggagtttgc gacgttgcga gtgaaagatg
    12061 ccatttgtga ccgatttgtc cgggcgacac atgcgcgccc cgaggtaagt aaacgacaac
    12121 ccgatattca aattgtggtc tttgtcgatg cagagcatgt ggtgtggtat atcgacacct
    12181 ccggagatcc gctgtttaag cgtggatggc gaaaggtggc cggagacgcg ccgatacgag
    12241 aaaatttggc ggcaggcatc ctcaggcttt caggttggac cggtgagcag gtcttgcttg
    12301 atccgatgtg tggcgccggc acatttttaa ttgaggcggc gttgatggcc aaagggattg
    12361 ccccaggccg tgggcgggaa tttgcctttc ggcacctgca gaactttgat gacacgatct
    12421 gggatcatgt acgccaagag tcgatcaaat cgattccccc ccgtccgggt ctttctattg
    12481 tgggatatga tgaggatgcg aatgccttga ccatggccag aagtaatctt gaaggcctgg
    12541 ggcttgagga tattcaattg ggtcaggttg atgtgttgga tgtgatgcct cctgcctcga
    12601 agggttttct ggtcaccaat cctccttatg gagtgagaat gggagaccgg tcggagttgg
    12661 aggagtggta tccgaaattt gggaatctgt tgaaacagcg gtttgcgggg tgggatgtct
    12721 atgtgctcac agccgattcg cgtcttccta agctgattca cttagcgccg tcgcgaaaaa
    12781 tccctctgtt caatggcccg ttggaatcga ggctgtatga atttcaaatg gtggctggag
    12841 gtaaccgcaa atcggcaaga caggccaggc aacaggcggt aaacaggcca ggtcttagat
    12901 gagaggtttt cctgccatgt ttaagagcga gacccagaca tcttcaatgc atcgcagcac
    12961 ataaatccat tcgtcgcagg tcttcggtcg tttgaaaaga tggcaattgg gagggaaaga
    13021 aaaatctttc gagggacgtg gagtctcttc cgaaaacaag gccaccggaa tggactgtaa
    13081 gcggcggtcg cgttgaagtc tcttaataat ttcttctccc gacacatccg ggagatgatt
    13141 ggagagaaaa atcaggtcgg gttgtggggc atccataaag ggatattcct gatttaaaaa
    13201 ggccatggct tgttcgccgt cttcggcttc atgaatttca acacagatga tttgggccac
    13261 cgtcagtgct tctttgaggg cttctatctg agcgatatca tcctcgatgg tcaggatctt
    13321 aaatgtgttt ttgacggctc cgcgcatgat ccgatgctca ttttcctggt tgaagcagac
    13381 tgcccacttt tcagtctggt gaaagattga cgtctgatat gcgatagaaa atacgtgtgt
    13441 tggcctgtct gttttttcgg ttgaatcttg ctgagattaa gagaaaaccg gctgagacgg
    13501 atttttaatg aagaagaatg gggaagagag tcccgatgcc tggaacatgt tctgttgatt
    13561 gcagaatgga tcgaggctca ttacgctgaa aaagtggtac accaactagc gtaaatttcc
    13621 ctcaatatga acaaacatga aacatgcaag caaagaggga tactctctta cccctatggg
    13681 gtgagagaaa gacacgtctc cggcctgatt gacatgctag ccagggaggc tgccgagcag
    13741 ttgttggatc accgggatgg catcagggcg agcggcgatg atgccattga cgatgggatg
    13801 aggttggtta cattgaatgg atttcaggtc tctgtctagg acgacccctc cggccgcttg
    13861 aaccagaagg agcgcagccg cgatatccca ttcgttttgg gtgccaatat tgattactcc
    13921 gtcaatctgt ccggcagcca ccagtgccaa tgaataagca atggaaccca taagattgcg
    13981 gcaattggcg gtttctcgcc aggtgcgaat ggtggagcgg tcaatgggtc ccgtattcac
    14041 caggaacgac aagcgctgtc cggtagtccg gccgacatga atggggcagc catttacggt
    14101 ggcctgttca cctcgtacgg cacaataata ttcccgggtg gcggggttga agataatccc
    14161 gatagaggcc tgtccatggt cgatgagcgc aagggaaatc gcaaattgtg ggaggtattt
    14221 cataaagggt ttggtcccgt caatgggatc caggatccac acacggtttt ttttcagtct
    14281 gatggggttg tcgggcgatt cttccgagag ccagccatca tcaggataga cggcgaacaa
    14341 ggcttcctgg acgatacgat tgacttccag atcggcattg gtcactggcg atccgtccgg
    14401 cttgatctgg acggctaatc cttcatactg gattctgtga aggcgatcac ttgcgcggac
    14461 cagggcttgt cggatgatgg agagttcttg g
//
LOCUS       DATIAL010000453         3064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1156683, whole genome shotgun sequence.
ACCESSION   DATIAL010000453 DATIAL010000000
VERSION     DATIAL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156683"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VLA60_14065"
     CDS             <1..700
                     /locus_tag="VLA60_14065"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HSF10533.1"
                     /translation="DSTLDYLAEGAYRLYCLSRIRVMVIDSLVLATGNRHKVEEIQSI
                     LNDVGIPMLTLNEFPDFQAVNEDGLTCQANAVKKAVATAKFTGHWALADDTGLEVDAL
                     QGRPGVYAARYAGEQATYEDNCQKLLQELHGVPSDQRTARFITVVALANPDGETETVE
                     GVLEGIITQEFHGTGGFGYDPVFYVPQLGKTLAEMTFAEKNRISHRAQAVTNAKSLLI
                     THRHSQESGRSAAR"
     gene            678..754
                     /locus_tag="VLA60_14070"
     tRNA            678..754
                     /locus_tag="VLA60_14070"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:712..714,aa:Pro,seq:tgg)
     gene            complement(769..1017)
                     /locus_tag="VLA60_14075"
     CDS             complement(769..1017)
                     /locus_tag="VLA60_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10534.1"
                     /translation="MTITLNSQNDRTIATLLQHSWTNLVQRYVHLSPTHLRNAVEGVS
                     GYGKARALDPQPKGEATIQDGTVAKSIIGVREEREKVV"
     gene            complement(1038..1346)
                     /locus_tag="VLA60_14080"
                     /pseudo
     CDS             complement(1038..1346)
                     /locus_tag="VLA60_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1421..1594)
                     /locus_tag="VLA60_14085"
     CDS             complement(1421..1594)
                     /locus_tag="VLA60_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10535.1"
                     /translation="MVPVLAICDGALGFWVAMEEDFPPHTPSALLCHKTRNALNALPK
                     SVQPKAKQAFQEI"
     gene            1593..2000
                     /locus_tag="VLA60_14090"
     CDS             1593..2000
                     /locus_tag="VLA60_14090"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF10536.1"
                     /translation="MSPRAFHAFAVVGNEDALEAFYETGRQWPVLGTEGFLGWVKTQV
                     PRLRLLREHPRYERANVHPSVRQVLQQVATVFGVEVSTVKRGRRGEANQARQVAMYLV
                     KRLCDLTLRETATHFGVESYGVVGWACAQVRAK"
     gene            2086..2406
                     /locus_tag="VLA60_14095"
     CDS             2086..2406
                     /locus_tag="VLA60_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10537.1"
                     /translation="MGYDWIFTGGGGIGSGLSKGSGALINAVKGTYRTTPANLAEKLA
                     LEEAKGGAGKRIMEGKIRDPRLSPESEWSKMKHVHSHSGGNETIIHYWKNLINGSSEG
                     FKFK"
     gene            2403..2876
                     /locus_tag="VLA60_14100"
     CDS             2403..2876
                     /locus_tag="VLA60_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10538.1"
                     /translation="MKRKVLQLAAASLVLGLLNGKNLPDIATEALVEGEDSYSLRILA
                     GLLEPSSSEGQMLLEKVFLELAIPKPNRIEAALHLSKEVAGKILEGQLSPYHGAKKID
                     EISLLVPLEDILPLHPFINWSSEWEERPEDHAFMDNAIIEAAKDLNSLIENEEKQ"
BASE COUNT          745 a          705 c          851 g          763 t
ORIGIN      
        1 ggattcaacg cttgattacc ttgcagaagg agcttatcgg ctctattgcc taagtcggat
       61 aagggtgatg gtcattgatt ctttagttct ggcaaccggg aatcggcata aagttgagga
      121 aattcaatcc attctcaatg atgtgggcat acccatgctc accttgaatg aatttcctga
      181 ttttcaggct gttaatgaag atgggcttac ctgtcaggcc aatgccgtga aaaaggccgt
      241 ggccacagcc aaatttaccg gccactgggc cttggcggac gataccgggt tggaagtgga
      301 tgcgttgcag ggacgtccag gggtctatgc ggcccgatac gccggcgagc aagcaactta
      361 cgaggacaac tgtcaaaaat tgttgcagga attgcacggg gtgccttcgg accagcgaac
      421 cgcccgtttt atcaccgtgg tggccctggc caacccggat ggtgagacgg aaaccgttga
      481 aggcgtgtta gagggaatta ttacccagga gtttcatgga acgggtggat tcgggtatga
      541 tccggtattc tatgttcctc aattaggaaa aacactagcc gagatgacct ttgcagaaaa
      601 aaatcgaata agccatcgcg ctcaagcggt gaccaatgcc aagagtcttc tcatcacaca
      661 tcgccattca caagagtcgg ggcgtagcgc agcccggtag cgcgcctgct ttgggagcag
      721 gatgtcggag gttcaaatcc tctcgccccg accaataaaa acaacaattt agacaacttt
      781 ttccctctcc tccctcacac ctattatgga ttttgctacg gttccatcct ggatcgtggc
      841 ttcccccttc ggttgtgggt caagagctct ggctttgcca taccccgata ccccttccac
      901 ggcgtttctc aggtgggtcg gggagagatg gacatagcgt tgaaccaaat tggtccagct
      961 atgttgaagc agggtggcga ttgtcctgtc attctggcta ttcagagtta ttgtcaaatg
     1021 tatttctgga gcgttgctca agcgccgact gagtccgtcg ttagttgttc tatcccatcg
     1081 gtaaattgga tgcccgcgat gactttgggc aactccttca atcctggtaa tcgtctccag
     1141 gttttctcgg cacactcccc caacttaaac atcctgtgcc gcatgccgtc acgcctgaga
     1201 tatctcttcg tgcgggctgt ccgatgtcga atcgttccaa acgtcgattc aatggggttc
     1261 gtcgtccgga ggctcggcca atgggcagcc ggaaaggcat agaccatgag cagctccttc
     1321 ctatcttttt ccaagcatgc ggtggctttg gggtatttgt gtcataggtg ttgaggaatc
     1381 cttcgaagga ctgctccgcc tcccttttgg tctctgcccg ctagatttct tgaaacgcct
     1441 gcttggcttt tggttggaca cttttcggca gggcattcaa ggcattccgt gtcttatgac
     1501 ataacaacgc tgatggcgtg tgggggggga agtcttcctc catcgccacc caaaagccca
     1561 gggcgccatc acagatagcc aatacgggaa ccatgagccc tcgggccttt catgcctttg
     1621 cggtggtcgg aaacgaggac gcgttggaag ccttttacga gactggacgc caatggccgg
     1681 tgttgggcac ggagggattt ctgggatggg tcaagaccca ggtcccacgt cttcgactcc
     1741 tgcgggagca tccacgctat gaacgggcga atgttcaccc gtcggtccgt caggtcctgc
     1801 agcaggttgc cacggtgttt ggcgtggagg tctcgaccgt aaagaggggc cgtcgcgggg
     1861 aagccaatca ggctcgacag gtggcgatgt atctggttaa gcggctgtgt gatctgactt
     1921 tgcgggaaac ggcgacgcac tttggggtgg aaagctatgg ggtggtaggg tgggcctgtg
     1981 cgcaggtccg ggcgaaatag ctcagcgact cccgcttcaa gaaacgggta gggcaggtgg
     2041 agacagcaat gagtcaacaa aagatttgac ccctaatctg agtgggtggg atacgattgg
     2101 atttttaccg gggggggagg cataggctcc ggacttagca agggatcggg agcacttatt
     2161 aatgcggtaa aaggtaccta tagaacaacg ccagcaaatt tagccgaaaa actggcccta
     2221 gaagaagcaa agggtggagc agggaaaaga attatggagg gaaaaatcag agatccacgc
     2281 ttatctcccg aaagtgaatg gtccaaaatg aagcatgttc attctcattc tggtggaaat
     2341 gaaacaatta ttcattattg gaaaaatctc ataaacggtt caagcgaagg atttaagttt
     2401 aaatgaaaag aaaagttttg caattggctg cggccagtct ggtcttgggc ttgctcaatg
     2461 ggaaaaatct acctgatatc gcaacagaag ctctggtgga aggagaggat tcatattcat
     2521 tgaggatttt ggctggcctc cttgagccat cttcctcaga aggacaaatg cttctagaaa
     2581 aagtatttct ggaactggca atacccaagc caaatcgaat tgaggctgca cttcacctct
     2641 caaaggaagt tgcagggaaa atacttgaag ggcaactttc accctatcat ggggccaaaa
     2701 aaattgatga gatttcttta ttggttcctt tggaagacat tcttcccctt caccccttta
     2761 ttaattggtc tagtgagtgg gaagagcgcc cagaagatca cgcttttatg gataatgcga
     2821 ttattgaagc ggcaaaagat ttaaattccc ttattgaaaa tgaggagaag cagtagcagg
     2881 tagcttgggg actaggaata ggttgaatgt aaatatcttg aatgtgtgac ggagatgttt
     2941 gttttttgac tgttgtcggg gatatctcag agaaatacgt cggccatagt caccggcccg
     3001 ttgacttgcc atggcttgct tcacggcaga gatcgggcgt cccatgcagg tcacgaaacc
     3061 gctt
//
LOCUS       DATIAL010000454         5159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1156738, whole genome shotgun sequence.
ACCESSION   DATIAL010000454 DATIAL010000000
VERSION     DATIAL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156738"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VLA60_14105"
     CDS             complement(<1..639)
                     /locus_tag="VLA60_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HSF10539.1"
                     /translation="MVTGAVAGGVLGALAPEAARHVEVLGELWLRMLRMLVVPLIVAS
                     IIQGVGSLGDIRQLGQLGGATVTYYLITSGMAIVLGVILVSLIKPGVGADIAAAVAPS
                     GVEAAVNIGWLDLIKEFLSPNLFASASQGELLPLVIFSLAFGAALTTVGENGTLVLKF
                     FEGIFGAIMKLIHLVMWIGPIGVFGLVAGRLASAGGLDGIVALLVGLGLFTVT"
     gene            complement(854..1003)
                     /locus_tag="VLA60_14110"
     CDS             complement(854..1003)
                     /locus_tag="VLA60_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10540.1"
                     /translation="MSFLQVRQSTLTGERQTFISASAIAYIQELKPDQDLGWQIGVEP
                     SRALI"
     gene            complement(1042..3036)
                     /locus_tag="VLA60_14115"
     CDS             complement(1042..3036)
                     /locus_tag="VLA60_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10541.1"
                     /translation="MSDSPEGSPQTELQAATARQEQALLQALEALEQATPFVKAKYQP
                     EVIRRATDLFETDAGLQFVRAQAHRFDSAGVFHAGPWEHPDRLLPALVGAGLRAEGQY
                     SSLEMLSELRVLAIAAGESPHPKFSAEQALTFLRTVMGLNLDLLYGSETEESRLRPKV
                     YARAHRILAMIKHEISSEGLLEQVLDDIDARVAQRPIDVSLTLKMIEQAENIKGDPDP
                     KLAARLDRYLKATGPPTPLAKKAGSPTNYGKLLAKATTHEIENEAKVVGKLLTLTGLS
                     SEYHVAFLQHALKHEETGILATALDLTEVAQAHMEQYREKVFELMRLTIHPATSWIIY
                     GFQQMLERMLLARPEVADGLTKLLNLDLCPTAQQVIKKQLPRGSGITANAAIVGGAIA
                     MLGQPLGVGQGNNPTCQGARALSLWSLHDPGYLLQLLTSAARDDTVEFMFEGVPILSG
                     EIGGGVAEGKFDLKLDPVSRILVPHLDRIYDEKMKRAALRGEDPHKWVNPALYGRWVA
                     TSLASITMVNQTVSGYEDFLRLFYATHHPLYDGGHDLVYPNPVGLLITNVHGVFLGYH
                     AVSIQRVAEDEEGKVRVYFFNPNNEGRQNWGRGVEPSVVGHWEIPGESSLPFEHFAAH
                     IYAFHYNQMEVGDPKAVPAEIIAEVTAHAKEGWGQAFTWL"
     gene            complement(3029..4276)
                     /locus_tag="VLA60_14120"
     CDS             complement(3029..4276)
                     /locus_tag="VLA60_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HSF10542.1"
                     /translation="MEKNAPVVKKSRGGKRAPVALLGYSLETMEAAKEHNSPFVAVVP
                     EGFDIALKEDGVDVVTWDFYVHNHNSDRLYEKLLEREVSHAVPLYEETVEWAGSLNTR
                     LLKDPRAFQRALLFRDKAMMKRNAQMNGIRVGVFEEIRSREEALAFFDDINEARLRLQ
                     EEAPEPVHLKPLSAAGSVGHVFVRSRQDITSLPDDAFPCLAESHLAGQEFSVEAFIHK
                     GKIEFMNITEYVHLGYSQIVPETPRLTKHRTKIRKAIEKLLKAFGIVNGMIHPEYFLD
                     QEGELHFGEVAARVPGGHIFELIQRAHGFNPYAALLLCSDPNTSKTELDRFFPKEKDH
                     KIFAANLMVYPRKASVHQLQFPKELEAHPYMDRHTMFEPVTAKVAERVGFGNHYGTVF
                     LAGEDPETLRELVQRYEEYDFYE"
     gene            complement(4395..4961)
                     /locus_tag="VLA60_14125"
     CDS             complement(4395..4961)
                     /locus_tag="VLA60_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF10543.1"
                     /translation="MMQNQTELGERLHRRSGRRIIHRGLDIQPAKRHEMVIAANILRS
                     SADWYRPFLHEKDMTQHEVDESWGGREFERRQFYIGREEQAPVGIVSTQSVGDMFYIG
                     YIYVYDHQTGKGFGPQLLNHVRDVAWREGKHGMVLIAHPKAYWATRSYRRFGFECIAT
                     TRDQVLEWRGGWLKPYYEEGFELYQYLF"
BASE COUNT         1273 a         1460 c         1220 g         1206 t
ORIGIN      
        1 cgtaaccgtg aataacccta aacccactaa cagcgcgaca ataccgtcaa gtccaccggc
       61 actcgctaaa cgccctgcca ccaagccaaa cacgccaatg ggaccgatcc acatcaccag
      121 gtgaatcaac ttcatgatcg ccccgaatat cccttcaaaa aacttgagca ccaatgtacc
      181 attttcaccg accgtcgtca gagccgcccc gaacgccaga gaaaagatta ccaatggcag
      241 caattccccc tgcgaagcgg acgcgaataa attcggggaa aggaattcct ttatgagatc
      301 cagccatccg atgttcactg ctgcctcaac tccggaaggg gccaccgctg cggcaatatc
      361 ggctcccact ccaggtttaa tgagactgac cagaatcacc cccagcacaa ttgccatgcc
      421 tgaggtgatc aaataatacg taactgtcgc tcctcccaat tgacccaatt ggcgaatatc
      481 acccagtgat ccaaccccct ggataatcga agccacaatc aaggggacca ccagcatccg
      541 caacattcgg agccagagtt cccctaggac ttccacatgg cgagcggctt ccggagccag
      601 ggcacccagc accccgccgg cgaccgcacc ggtcaccatg agccacacaa gaggaccatg
      661 gccatttcca cgatctacag aaatgggttg ttcggtaatg ggacaaacct gaggttaagg
      721 aaaggaaatg ttatttggtc tttttagctg atttctgttc gggtaccctg actccccgag
      781 tattggtctt tgcctgcctc acaaggtcat cggaactatc gagggaaata atgggctcaa
      841 gcttatgatg caattaaatg agcgctctgg agggttccac accaatttgc caaccaagat
      901 cctggtcagg cttcagttct tggatatatg caatggctga tgcgctgata aaggtctgtc
      961 gttcccctgt cagtgtcgat tgcctgactt gtaaaaaaga catactccac caccccttcc
     1021 aaatttctaa ggcccatagg attataacca ggtgaaggcc tgtccccacc cttccttcgc
     1081 atgggctgtc acctcggcga tgatttcggc tggaacggcc ttggggtccc ctacttccat
     1141 ctgattatag tgaaacgcat agatatgcgc ggcaaaatgc tcgaagggta atgacgattc
     1201 tcctggaatt tcccagtgac cgaccacaga cggctcaacc cctcttcccc aattttgacg
     1261 gccctcgttg ttgggattga aaaaatagac acgaaccttc ccttcttcat cctcggccac
     1321 acgctggatg gagacggcat gataccctaa aaaaaccccg tgaacattgg taataaggag
     1381 cccgacaggg ttggggtata ccaaatcatg ccccccgtca tataaggggt gatgggtggc
     1441 ataaaataac cgtaaaaaat cttcgtaccc ggaaacagtc tgattaacca tcgtaatgga
     1501 agccagactg gtcgccaccc accgaccgta caaagccggg ttcacccatt tgtgtgggtc
     1561 ctcgccgcgc aaagccgcac gcttcatctt ttcatcgtaa atccggtcga gatgaggcac
     1621 cagaatgcgt gagacgggat cgagcttcaa atcaaatttt ccttccgcga cacctcctcc
     1681 aatttctccg gataaaatcg gcactccctc aaacataaac tcaacggtgt catcccgagc
     1741 cgcgcttgtc agcaattgga gcaaatatcc aggatcatgg agactccaaa ggctcagggc
     1801 tcgagcaccc tgacaggtcg ggttgtttcc ttgccccact cccaacggct gccctaacat
     1861 ggcgatggca ccacccacaa tggccgcatt agccgtaatg ccgctccccc gaggcagctg
     1921 tttcttaatc acttgttggg cagtaggaca taaatcaaga ttaagcaatt tggtcagacc
     1981 atcagcgacc tctggtcgtg ccagcagcat ccgctccaac atctgttgaa atccataaat
     2041 aatccaactg gtggcgggat gaattgtcag gcgcatcaac tcaaagactt tttcccgata
     2101 ttgttccata tgggcctggg caacctcggt taaatccaag gcagtcgcca ggattcccgt
     2161 ttcctcatgt ttgagggcat gctgaaggaa cgcgacatgg tattcgctgg ataacccggt
     2221 cagagtcaat aattttccaa cgacctttgc ctcattttct atctcatgtg tggtggcttt
     2281 ggccaagagt ttgccgtagt tggtgggaga gcccgctttt ttggccagtg gtgtgggagg
     2341 acccgtggcc ttgaggtacc ggtcgagtct ggcggccaac ttgggatcag gatctccctt
     2401 gatgttttct gcctgctcga tcatttttag ggtgaggctc acatcaattg gtcgctgagc
     2461 cactcgcgca tcgatgtcat caagaacctg ttccagcaac ccttccgagg aaatctcatg
     2521 cttaatcatg gctaatattc tgtgcgcccg tgcatagacc ttcggacgaa ggcgagactc
     2581 ctcggtctca ctgccataca acaaatcaag attaagcccc atgactgtcc gtaaaaaggt
     2641 gagggcttgc tccgcagaaa atttcgggtg gggcgattca cctgctgcaa tggctaacac
     2701 ccgaagctca ctcagcattt ccaaagacga atactgccct tcggcacgga gccccgcgcc
     2761 caccaacgcg gggagaagcc ggtcgggatg ttcccagggt ccggcgtgaa agaccccagc
     2821 ggagtcaaag cgatgggcct gtgctctcac aaattgcagt cctgcgtcgg tttcaaataa
     2881 atccgtggca cgacgaatga cctctggctg gtacttagcc ttcacaaatg gcgtcgcctg
     2941 ttccaaggcc tcaagggcct gaagtaaggc ttgctcctga cgggcagtgg cagcctgcaa
     3001 ttcagtctga ggggagccct cgggtgaatc actcatagaa atcatactcc tcataacgct
     3061 ggacgagctc acggagagtt tccggatctt ctcccgccag gaagacggtt ccataatgat
     3121 taccgaatcc cacgcgctct gccactttgg cggtgacggg ctcaaacatg gtatgccggt
     3181 ccatatacgg atgggcttcc aattctttag gaaactgaag ttgatggacg ctggctttcc
     3241 gaggatacac cattaagttg gccgcaaata ttttatggtc tttttctttt gggaaaaacc
     3301 ggtctaattc tgttttggag gtattgggat cggagcataa caacagagcc gcatacggat
     3361 taaacccatg ggcacgctga atgagttcga aaatatggcc gccgggaaca cgggccgcca
     3421 cctccccaaa gtgcaactcc ccctcctggt ccaagaaata ttccggatga atcataccat
     3481 taacgatgcc aaatgccttc aaaagttttt caatcgcctt acgaatcttt gtccggtgct
     3541 tagtgagcct cggcgtttcc ggcacgatct gggaatagcc caaatgcacg tactcggtaa
     3601 tattcataaa ttcgattttt cccttatgga taaaggcttc gaccgaaaat tcctgcccgg
     3661 ccaaatggct ttcggccaga cacggaaagg catcgtcggg aagggaggtg atgtcttgcc
     3721 ggcttcgaac aaacacatgg cccacactgc ccgccgccga cagaggtttc agatgaaccg
     3781 gttcgggcgc ttcctcctgc aagcggagtc tggcctcatt gatgtcatca aaaaacgcga
     3841 gagcttcctc ccgcgagcga atctcttcga acaccccgac tcgaatgccg ttcatttgcg
     3901 cattgcgctt catcatggcc ttgtcgcgga acagcagtgc ccgttggaag gcacgtggat
     3961 ctttcagtag gcgggtgttg agggacccgg cccattccac cgtttcttca tacagcggca
     4021 ccgcatggct cacctcccgc tccaacaatt tttcatagag tcggtcggag ttgtggttgt
     4081 ggacataaaa gtcccaggtc accacatcaa ccccatcctc tttcaatgcg atatcaaaac
     4141 cctccggcac caccgcgacg aagggactgt tgtgttcctt tgcggcttcc atcgtttcca
     4201 agctatagcc gagcagagcc accggcgccc ttttccctcc acgggacttc ttgaccactg
     4261 gtgcgttctt ttccatgcag acctcctgta ggtaggggac atatcgaata aactcctcca
     4321 tcctgtcccc gcttgcagac aaatggaaga acccatcagc aataatgatt cgctcgtgtg
     4381 tgaggaaagg tgtgttagaa cagatattgg tacagttcaa aaccctcttc atagtagggt
     4441 ttcaaccaac cgccgcgcca ctctaggacc tggtcacgag tggtcgcaat gcattcaaag
     4501 ccgaaccggc gataggagcg cgtggcccag taggccttgg gatgggcaat taacaccatg
     4561 ccgtgttttc cctcgcgcca agccacatca cggacgtgat taagtaattg aggcccaaac
     4621 cctttgccgg tttggtgatc atacacatag atatatccga tataaaacat gtctccgact
     4681 gactgcgtag acacgatgcc tacgggtgcc tgctcttccc gcccgatata aaactgccgc
     4741 cgctcaaact ctctcccccc ccaggattca tccacctcat gctgcgtcat atccttttca
     4801 tgaagaaaag gtcgatacca gtcggcagaa gaccggagga tattggccgc aataaccatc
     4861 tcatgacgtt tggccggctg aatatccagg cctcgatgaa taattctcct tccgcttctt
     4921 cgatgtagcc gctccccaag ttccgtctga ttttgcatca tgaagatcta tcctttttta
     4981 aaaaatttgt gccacggact catactcctc acgagtcgag ccggagggtc attgcctatt
     5041 gtgaataatt attttcagag aaaaattggt gtgagcatct catcattcac accggcattt
     5101 tcacattgtg taagatgtgt caaatcttga atgtttgtga cgaaaacaaa cggtaccct
//
LOCUS       DATIAL010000455         3404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_405864, whole genome shotgun sequence.
ACCESSION   DATIAL010000455 DATIAL010000000
VERSION     DATIAL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405864"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..1654
                     /locus_tag="VLA60_14130"
     CDS             323..1654
                     /locus_tag="VLA60_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10544.1"
                     /translation="MGISDALRTRSGFPITTISIRTLPSFLKFLFVLLLIMPLSGCSR
                     HPVTTPDHAPNAESAIIVVPGYYGTRLVRESDGSLVFISLNQVLFGSQTLTIPVPGLG
                     FGETTALQPEGILDEVRVVPLLYSVDVYGSLLERLRSFTNTSRDVIPFTYDWRGDLME
                     AVRSLDVLIRRHRGEGKKEISVVAHSMGGLIVSYYLRYGTQDIDTAVETWEGAGEISR
                     VVMAGVPFLGAMNSFRNMNYGATFGWNSSLLSYEAYASFPASYYLLPVADSDELLTPE
                     LKPLHGVIRNAGQWRQSAWGLLKNKQTLSKEIVDGRAAYLSFWLRQSEQFLERLHAPL
                     SRPSPHQPSLLYLYAKGTPTLAKGVWTGDPGKGHDSLLFEDPDPVAGPAGNHPTVYAD
                     GDGTVTVSSVWLPEAFRQSFHPTIRDYEVGHTELVTTPDIQDDIIKFLDRR"
     gene            1729..2973
                     /locus_tag="VLA60_14135"
     CDS             1729..2973
                     /locus_tag="VLA60_14135"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSF10545.1"
                     /translation="MNLSLLILLGVYLVIAFRKIGGIPVKIWQAMTAGAVLVLMTGQI
                     SPLAAIQAINLDVMVFLLGMFVVGEALILSGYLSSLAYGILHRVKSADGLVLMILFGA
                     GLTSAVLMNDTLAIVGTPLVLRLAREHRLEPRLLLMTLAVAVTTGSVLSPIGNPQNLL
                     IAIDGPVPSPFSTFLQALALPTFLNLVVAYVVLRVMWPRAFHATTLVHNVVAISDPAL
                     ARLARLALFVIIGLIGVKIGCVSLAVPLEFPLSYLAMAAALPLLVCSRRRLELLRQVD
                     WSTLLFFAAMFVLMASVWQTGVFQEWTAHLQMDLTSLPSILSLSIILSQLISNVPLVA
                     LYLPLVSEAGGSLPGLLALAVGSTIAGNLMILAAASNVIIIQRAEQEGYTLSFRIFAW
                     IGVPLTILQTLVYAVFLEWGVG"
     gene            complement(3039..>3404)
                     /locus_tag="VLA60_14140"
     CDS             complement(3039..>3404)
                     /locus_tag="VLA60_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10546.1"
                     /translation="LISIKMKPLHSSVRAIIVSTIMSLIPFLPALAMEERAQPGKALF
                     QQYCQDCHGPQRDGRGVLRPFLEQDPANLTSQMTQAKTDQELFSIIKKGGGEMHGWAD
                     TFTDEQVLDLVHYIRALSP"
BASE COUNT          652 a          873 c          942 g          937 t
ORIGIN      
        1 aaccaggcga atgaagcgct cagcagtgcg ctgctcagga gagaccatca tctggactga
       61 ctggcgttca gatgcttggt ctccatccca tggaaatcac taacattcac cttacatttg
      121 acgtcaatca tctttccacg cctcatccat attttttaaa ctaagcaggc attcggccgc
      181 tcctctccga ttcgacctgt tcttctctga caccttatgg agagtggaca gggtaggact
      241 tcggtaatct ttaccaaatg tatgatgtag agccatttaa tcttaatccg cagggttatc
      301 ggcaacgacg gtcagaaaat ctatgggtat ctctgatgca ttacgcacac ggagtggttt
      361 tccgattacc acgatttcca ttcgaacact accatccttc ctgaaatttc tgtttgtatt
      421 actgctgatc atgccgctgt ccggatgtag tcggcaccct gtcaccacgc cggatcatgc
      481 tcccaacgct gaatcggcga tcatcgtcgt ccctgggtat tacggcacgc gtttggttcg
      541 cgagtcggat ggcagtctgg tgtttatttc tctcaaccag gtgctgttcg gttcgcagac
      601 attgactata cccgtgcccg gcctgggttt tggagaaacg accgccttgc aacccgaggg
      661 cattcttgat gaggtccggg tcgttccgct cctctactcc gtcgatgtct atggttcatt
      721 gctggaacgg ctacggagct tcaccaatac aagccgggac gtgattccgt ttacctatga
      781 ttggcggggg gatctcatgg aggctgttcg aagtttggat gtcctcattc gccgacaccg
      841 gggcgaggga aagaaagaga tctctgttgt tgcgcatagt atgggtggcc tgattgtcag
      901 ctactattta cgatatggca cccaggatat cgatactgcc gtggagacct gggagggagc
      961 aggggagatc agccgcgtgg tgatggccgg ggtgccattc cttggagcca tgaactcgtt
     1021 tcgaaacatg aactatgggg cgacctttgg ttggaactcg tctctgctca gctacgaggc
     1081 ttatgcgtct ttcccggcca gttactatct cttaccggtt gccgattctg atgaactgct
     1141 taccccggaa ctgaagccct tacacggggt gatcagaaat gccggacagt ggcggcagtc
     1201 cgcctggggg ctgctcaaga ataaacaaac cttatctaaa gaaatcgttg atgggcgtgc
     1261 cgcttacctg tccttttggc ttcgtcaatc ggaacagttt ttggaacggc ttcacgcacc
     1321 tctctctagg ccaagccccc atcaaccctc ccttctgtac ctgtatgcga aaggcacacc
     1381 gaccttggcc aaaggggtgt ggacgggaga ccctggaaag ggccatgatt ccttgttgtt
     1441 tgaggatccc gacccggtcg cgggtcctgc agggaatcat cccactgtct atgcggatgg
     1501 agatggaaca gtgacggtgt catccgtttg gcttccggag gcctttcgtc agagctttca
     1561 ccccaccata cgggactatg aggttggaca taccgaattg gtcactaccc cggatatcca
     1621 agacgatatc ataaaatttc tcgatagacg gtagcacacc atgggctatc ctagtcacct
     1681 cttttctcac tgcgggttcc atgaacttgt cttgacgacc tctgccgcat gaatctttcc
     1741 ctgctcattt tgctgggtgt ttatctggtc attgcattcc ggaaaatcgg tggcatccct
     1801 gtgaagatct ggcaggccat gactgccggt gccgtattgg ttctgatgac cggccagatt
     1861 tcccctcttg cggccataca ggccatcaat ctggatgtga tggtgttttt gcttgggatg
     1921 tttgtggttg gggaggcgtt gattcttagc ggatacctgt cttcgctcgc ctacgggatc
     1981 ttgcatcggg tgaaatcagc tgatgggttg gtcctgatga tcctattcgg ggcagggctc
     2041 acttctgcgg tgttgatgaa cgatacgttg gctatcgtcg ggacgccttt ggtactgcgt
     2101 ttagcccgtg aacatcgcct cgaaccccgc ttgctcctca tgacgttagc tgtggccgtg
     2161 acgaccggga gcgtgttaag cccgatcggc aaccctcaaa atctcttgat tgcgattgac
     2221 gggccggttc cctctccctt ttcgactttt cttcaggccc tggcgcttcc cacttttctc
     2281 aatttggtgg tggcatatgt ggtcttacgg gtcatgtggc cgagggcgtt tcacgccacc
     2341 actctcgtgc ataatgtggt ggccataagc gatcctgccc tggcgcgctt ggcccgtctg
     2401 gcactgttcg tgataatcgg cctgattggg gtgaagatcg ggtgtgtgtc gctggctgtg
     2461 ccgctagagt ttcctttgag ctatctggcc atggcggccg cgttgccttt gctggtgtgt
     2521 agtcgacggc gtctggaatt gctgcggcag gtggattggt ccacactgct tttttttgcc
     2581 gccatgttcg tcttaatggc gagtgtctgg cagacgggag ttttccagga gtggacagcg
     2641 cacttgcaga tggacctcac ctccctgccc tccattctta gtctcagtat cattctgagc
     2701 caactgattt cgaatgtccc gttggtggct ttatatctgc ctctggtgtc cgaggccggt
     2761 ggttcccttc cgggccttct ggctttggct gtcggaagta cgattgcggg gaatctgatg
     2821 attcttgctg cggccagtaa tgtaattatt atccagaggg cggagcagga agggtacacc
     2881 ttgagtttca gaatttttgc ttggatcgga gtgccgttga cgattctcca aacgctggtc
     2941 tatgccgtgt ttcttgaatg gggtgtgggg taggtgagtg ttgcagaaag ctccatcacc
     3001 cgtttcgtgc gttacagcga tgctgtgcgg caaaacatct acggtgagag tgcccggatg
     3061 taatgcacca ggtccaggac ctgttcgtcg gtgaaggtgt ccgcccagcc atgcatttcg
     3121 cctccccctt ttttgattat ggaaaagagt tcctggtcgg ttttggcctg tgtcatttgc
     3181 gaggtcaaat tggcaggatc ctgttccagg aaaggacgta ggacgcctct cccgtccctc
     3241 tgaggaccat ggcaatcttg acaatattgt tgaaagagcg ctttgcccgg ttgggcccgc
     3301 tcttccatgg caagagccgg gagaaagggt atcaggctca tgatcgtcga gacgatgatc
     3361 gctctcacag aagagtgcaa aggtttcatt ttgattgaaa tcaa
//
LOCUS       DATIAL010000456         9127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_511973, whole genome shotgun sequence.
ACCESSION   DATIAL010000456 DATIAL010000000
VERSION     DATIAL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9127
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511973"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VLA60_14145"
     CDS             complement(<1..185)
                     /locus_tag="VLA60_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10547.1"
                     /translation="MAATRLFNNILGKVSFVVGIGFLWGLILPLAPVHSKESPTSEPP
                     LSASSPISGTILIVGNGP"
     gene            complement(372..665)
                     /locus_tag="VLA60_14150"
     CDS             complement(372..665)
                     /locus_tag="VLA60_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HSF10548.1"
                     /translation="MPLYEYVCEGCDHRYEAMQALSVKPEETLCPKCNTTQSRRIMSS
                     FASKIVGTHKPGFAEGKAYNMLNERMDKFSKLPPIMGQRAAPTEANSQPSGGE"
     gene            867..1046
                     /locus_tag="VLA60_14155"
     CDS             867..1046
                     /locus_tag="VLA60_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10549.1"
                     /translation="MSVLIRKFKLPTGMVKEERIDDPDRIERYMQFFDKEQQAKLDAG
                     QKVFLDKDEWQFVDN"
     gene            1165..1368
                     /locus_tag="VLA60_14160"
     CDS             1165..1368
                     /locus_tag="VLA60_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10550.1"
                     /translation="MGYWIHLKRSLDEAMIHRDRCPEVPSGPHRSDFWEGGWTEYPDK
                     DTALEYLENTDASLQLKCPLCKP"
     gene            1441..1875
                     /locus_tag="VLA60_14165"
     CDS             1441..1875
                     /locus_tag="VLA60_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10551.1"
                     /translation="MAEKFANARWVKEGFLDNRIVGSVIGQITFAGLDTVDVYLVGEF
                     VGEIAGKVIGFRNPRFLDDPQAGESLYDFSVPQLGKVSLMSFDPHPLLEPHPYFEWFS
                     AHQQHYRIELQPDEAWLLSEIERDAVEDQAQRIRQELTPLLP"
     gene            2041..3078
                     /gene="hemE"
                     /locus_tag="VLA60_14170"
     CDS             2041..3078
                     /gene="hemE"
                     /locus_tag="VLA60_14170"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HSF10552.1"
                     /translation="MNDRFLKACLRQPVDRTPVWFMRQAGRYMEEYQAIRRKHSILEV
                     CKTPALAAEVTLQPVNRFDLDAAIIFADILLPLQPMGLNLEFVEGKGPVIDNPIRGEM
                     GIVDLRPVDGEAFAYAGEAIRQALQALDNRVPLLGFAGAPFTLASYAIEGGSSRDYAR
                     TKHLMFSQPTVWHQLMSKLSVVVVEYLRVQSRAGAQAIQLFDSWVGCLSPGDYEEYVL
                     PHVQNIFSALRVEGIPLIYFGTGTTGLLSLMRKAGSDVMGVDWRVRLDEAWGTIGFDV
                     SIQGNLDPLVLFAPEKEIERRVKDIMQQAGGRPGHIFNLGHGILPNTPLHSVEFAIEC
                     VRRYSTASSRE"
     gene            3189..4688
                     /gene="hemG"
                     /locus_tag="VLA60_14175"
     CDS             3189..4688
                     /gene="hemG"
                     /locus_tag="VLA60_14175"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HSF10553.1"
                     /translation="MRKNPYQVVVVGGGISGLATAYALTEEAEKTQTPVQCTVVEREP
                     RWGGKILTHVTDNYLIEGGPDSFLTSKPWALELCRTLGLQDQLICTNPQHNQTFSFCR
                     GALRELPQGLLAFRPRRVDTLVSSGLLSWGGMLRMAAERFWPKQNPWPADESLGAFFR
                     RRFGTEAFEYLIEPLVAGIYAGDADELSIESTFPRFRELEREHGSVIKGMRKALASAP
                     PAPRSSGAATTMFMSLRGGLSELIQAVVEALRKRGVGLMEGVGCLEIQSRTLESGEFQ
                     VMLENGNRLPADAVVLATPAYQTARLLRVFRPETASLLDGIPYASTATISMGYPAESI
                     ASQIRGFGFVVPRKEQRPLLAATWTSLKWPDRSRAGETLIRCYIGGRGRETIVEQDDS
                     SLVKCIRGELLSMVGITAAPTYTEVHRWRQGMPQYVLGHRDRLAKVQEQLAHSPGLYL
                     TGAGMHGIGIPDCIREGTKVGKQLLQDYAANRAMKNSGSTESNGRGQIG"
     gene            4702..4992
                     /locus_tag="VLA60_14180"
     CDS             4702..4992
                     /locus_tag="VLA60_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HSF10554.1"
                     /translation="MEAIVRAHACVSGKVQGVGFRAFVQMQANKMVLHGWVRNRAEGG
                     VELEVEGPEASVHTFLEAIHKGPPLSQVLQVTVDWKDPNRQTEGFHVLRTSL"
     gene            5077..5592
                     /locus_tag="VLA60_14185"
     CDS             5077..5592
                     /locus_tag="VLA60_14185"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HSF10555.1"
                     /translation="MNYKAHIREVPDFPKPGILFYDITPLLNNPVCFRSIIDECICHY
                     QDASITRVVGIESRGFILGSPLAYHLNAGFVPVRKPGKLPADVFEVNYNLEYGSTALA
                     IHRDAIQKGERVLVVDDLLATGGTAGATVHLIRQLGGEIAGVVFLVELLKLDGRNKLN
                     GCDIHSLITYE"
     gene            5702..6217
                     /locus_tag="VLA60_14190"
     CDS             5702..6217
                     /locus_tag="VLA60_14190"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSF10556.1"
                     /translation="MAERPVKKETKTTASAGTKKGPAKGPSKATSPTPASRKPAKAQT
                     EDRHVALKEILLAKREALIQEIKQQLGQSVTEEQQRRLEAAMDSGDQALVDLDREMGI
                     SLQEMRNRERQLIDDALDSLDEGTYGVCAECGGEINEKRLQALPFARLCVECKSKREL
                     MEKIERSEQRQ"
     gene            6419..7099
                     /gene="queC"
                     /locus_tag="VLA60_14195"
     CDS             6419..7099
                     /gene="queC"
                     /locus_tag="VLA60_14195"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HSF10557.1"
                     /translation="MSKIVVLASGGLDSTVTAAIAKSEGYELYFLTVDYGQRHRTEIE
                     CAQKISEYFKVKEHKIIQLDLRTFGGSALTDQIPVPTNRTGRDREQQIPVTYVPARNT
                     IFLSLALAYAEVLDASSIFFGANIRDYSGYPDCRPEFIQAFKEVARLGTRMGLEGQQV
                     DILAPLLFLTKREIIQKGHALGVPFQWTNSCYNPGLQNQPCGHCDSCVIRQEGFKEAQ
                     LPDPLLAG"
     gene            7157..7585
                     /locus_tag="VLA60_14200"
     CDS             7157..7585
                     /locus_tag="VLA60_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10558.1"
                     /translation="MNKHSRRTILKSVAGVLCWFVVGCSENGTNARESSPPATIAQAS
                     VEKDLPTEFKDGETKFNTFCSPCHGVQAVGTAQGPPLVHKIYEPSHHADFAFQRAAAQ
                     GVKAHHWKFGNMPKIEGVTADDVTQIIGYVRWLQRQAGIS"
     gene            complement(7841..>9127)
                     /locus_tag="VLA60_14205"
     CDS             complement(7841..>9127)
                     /locus_tag="VLA60_14205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HSF10559.1"
                     /translation="QALETLYGVTTNSLTFPMKMKGRTTRLTREQLMGYVRHPLASVR
                     RGAYHALFKVYGEHRDVLGEMYKSLVQDWGNEGLTLRHYASPMAVRNVTNDVPEKAVD
                     ALLKTCRKNAVIFQEYFRLKARLLKIKQFQRYDLYAPFAAKKSKYSFARAKELVMEAY
                     QAFSPELAQRAKRVLEENHLDAAIRPGKMGGAFCYSVLPTQTPYVLVNFTGEPRDVST
                     LAHELGHAVHAMMASEHSIFTFHSSLPLAETASVFGEHLLSDLLLQQEMDPKVRAGLL
                     VDQLDDAYATIMRQAYFVQFERQAHHMIQQGATIDALAQTYLTLLREQFGPRLHVDKE
                     FQWEWLAIPHIFASPFYCYAYSFGSLLVLSLFQRYQTEGPSFVPRYLQLLSGGGSASP
                     QDLLKPLQVDINSTTFWQAGFTRIRGLVNELERSMP"
BASE COUNT         2241 a         2098 c         2455 g         2333 t
ORIGIN      
        1 ggtccatttc caacgatgag aatggtccct gaaatcggag atgaagccga aaggggaggc
       61 tctgaggtag gagattcctt tgaatgaaca ggggccagtg gaagaatgag tccccagaga
      121 aaacctattc cgaccacaaa agaaaccttg cccaaaatat tgttaaagag ccgggtggcc
      181 gccatatttc ttaggatcct tagcaaggaa caaccgttgc gcaagtggtc gaaactacag
      241 aataagttat caccaagaat gaaaagcggg cacatctttc cggtttaggg aaataaatgt
      301 caaggggaga ggaatgtgga agttggaaaa tcttgggcgg tcaccattca gagggccgcc
      361 caagcttcat cctattctcc tccggagggt tgggagttgg cctcggtcgg ggccgcgcgc
      421 tggcccataa tgggggggag ttttgaaaac ttatccattc gttcattcag catattatag
      481 gctttgcctt cagcgaatcc tggtttatgt gtacccacaa ttttcgaggc aaacgaggac
      541 ataattcgtc ggctttgagt tgtgttgcat tttgggcaaa gggtttcttc cggttttacg
      601 ctcagggctt gcatggcctc ataacgatga tcgcaccctt cgcagacata ttcatacaat
      661 ggcataaata actcctttaa aattatacga ggatgaacat gaatggtacc tccgtcaggt
      721 tttcagtata tcgaagattg tattgacgtg gccaatagat aaatgaattg tttttggaaa
      781 tgtccagaaa agattgacgg caagtttatg atttcactat aatttttcgt tggtcttatt
      841 acagacaatt tttggaggaa tgagtcatgt cggtgttaat tcggaaattt aaattgccca
      901 ctggaatggt caaggaggag cgcattgacg atccagatcg cattgagcgg tatatgcagt
      961 tttttgataa agagcagcaa gccaagctcg atgccggcca aaaggttttt ttagacaaag
     1021 atgaatggca gtttgtcgat aactgaattc ctgcagggca gactccctct ccggtatatt
     1081 ccggccgggt tgttcccacg tcaatctttg atcgtgtctg tggggcaggt ccggctcacc
     1141 actcagtgaa ggagcaccac gactatgggg tattggatcc atttgaaacg atcacttgat
     1201 gaagcgatga tccaccggga tcgttgcccg gaggtgcctt caggcccgca tcggtcggac
     1261 ttttgggagg gtgggtggac cgaatatccg gataaggata cggctctgga gtatttggaa
     1321 aatactgatg cctccctcca actcaaatgt cccttatgta aaccgtaact cgtgtttgtg
     1381 gataatcggt tcatcagtcc gtgaacagga ttcttcatta ttttttaata atttttttgt
     1441 atggctgaga aatttgcgaa tgcccggtgg gtcaaggagg gatttttaga caatcggatt
     1501 gtcggatccg ttattgggca aattaccttt gccgggcttg atactgtgga tgtgtattta
     1561 gttggtgagt ttgttggaga gattgccgga aaggtgattg gttttcgtaa ccctcgcttt
     1621 ttagatgatc ctcaggcagg agaatcgtta tatgattttt ccgtccctca attaggaaaa
     1681 gtcagtctca tgtcgtttga tccgcatcct cttcttgaac cccatcccta ctttgaatgg
     1741 ttttccgctc atcaacaaca ttatcgtatt gagctgcagc cggatgaggc gtggctcctg
     1801 tctgaaattg aacgagatgc tgtggaagac caggctcagc ggatccggca agagctcaca
     1861 cctctcctgc catgaccaga tgcgggttta agttgtggcc ttgttgtacc ccaaggccag
     1921 gaatcaaatt ttcacggaaa ttggtggtcc caggaaaatg atctgtgcat attgaccagt
     1981 cacgccaccc ttggttgata tttattgact cactctttct ccattcgtag aatgcccgcc
     2041 atgaatgatc gatttttaaa agcctgcttg cgtcagcccg tagaccgaac ccctgtttgg
     2101 tttatgcggc aagctggacg atacatggaa gagtaccagg ctatccggcg caagcattct
     2161 attttggagg tatgcaaaac gcctgcgtta gctgccgagg tcacgcttca acctgttaat
     2221 cgctttgatc tggatgcggc gatcattttt gcggatattt tgttgcctct ccagcccatg
     2281 ggattaaacc tggaatttgt tgaggggaag ggtccggtca ttgataaccc gatacgaggc
     2341 gagatgggca ttgtggatct gcgtccggtc gatggtgagg cgtttgccta tgccggagaa
     2401 gctattcgcc aggcacttca ggctctcgat aatcgagttc cgttactggg atttgccgga
     2461 gcgccgttta cattagccag ttatgctatt gaaggcggca gctcgcgtga ctatgcccga
     2521 accaaacact taatgttctc gcagccgacc gtgtggcatc aattgatgtc caaactgtcc
     2581 gtggttgtcg tggagtattt acgagtccaa agtcgagcag gcgcccaagc cattcaactc
     2641 tttgacagtt gggtcggctg tttaagccct ggcgattatg aggaatatgt ccttcctcat
     2701 gtgcaaaata ttttttccgc cttacgggtt gaagggattc ccctgatcta ttttggaact
     2761 gggacgaccg gacttctttc gctgatgcga aaggcgggaa gcgatgtaat gggtgtcgac
     2821 tggcgagttc gtttggatga agcatggggc actatcgggt ttgatgtgag cattcaaggc
     2881 aatctggatc ccctggttct gtttgcgcca gaaaaagaaa tcgagcgtcg ggtgaaggat
     2941 attatgcagc aagccggcgg cagacctggt cacattttta atttgggtca cggaattctt
     3001 cctaataccc ctttacacag cgttgaattt gccattgaat gcgttcgccg gtattcaacg
     3061 gcttcctcac gagagtgatg tagcgatact ttccccctgt cagctttctc ccgtccctcc
     3121 atgaaaacct gtttatttca atgttttagc cacgtacatg catttcctgt ctgattgctc
     3181 gggacttcat gagaaagaat ccataccaag tcgtcgttgt cggaggaggg atatccggac
     3241 tggctacggc atatgccctc acggaggagg ctgagaaaac acagacaccc gttcaatgca
     3301 cggtcgttga acgagagcct cggtggggtg gaaaaattct gacgcatgtg acagacaact
     3361 atctgatcga aggtggacct gactcgttcc tgacttccaa gccctgggct ctggaattat
     3421 gccgcacgtt aggacttcag gatcaattga tttgtaccaa ccctcaacac aatcagacat
     3481 tttcattttg ccggggagca ttgcgagaac tgccacaagg cttactggcc tttcggcccc
     3541 gacgggttga tacgcttgtt tccagcgggc tcctatcctg gggtgggatg cttcgcatgg
     3601 ctgccgaacg gttttggcca aagcagaatc cttggccggc agacgaatct ttgggagcgt
     3661 tttttcgtcg acgatttggc accgaagcct ttgaatactt aatcgaacca ttggtggcag
     3721 gcatctatgc cggggatgca gatgaactca gtattgagtc gacctttcct cgatttcgcg
     3781 aattagagcg cgaacatggc agtgtcatta agggaatgcg aaaggctctg gccagcgcgc
     3841 caccagcccc gcgttcctcc ggcgcagcca ccaccatgtt tatgtcgctt cgtggtggat
     3901 taagtgagct cattcaggcc gtggtggagg cattgcgcaa acgcggagta gggttgatgg
     3961 aaggggttgg gtgtctggaa attcaatctc ggactctgga gtcaggggaa tttcaggtca
     4021 tgttggagaa tggaaatcgg cttccagctg atgcggtcgt gttggccact ccggcatatc
     4081 agaccgctcg cttacttcgg gtatttcgac ccgaaacagc gagtctgtta gatgggattc
     4141 cctatgcttc gaccgcgacg atttctatgg gctatcccgc tgaatccatt gcttcacaga
     4201 tacgaggatt tggatttgtg gtgcctcgaa aagaacaacg cccgttgcta gccgctacct
     4261 ggacttcgtt gaagtggccc gatcggagcc gggctggaga aacgctgatc cgttgttata
     4321 tcggaggtcg agggcgagag acgattgtgg agcaggatga tagctccctt gtgaagtgta
     4381 ttcgggggga attactctct atggtcggaa tcacggctgc cccgacctat acagaagtcc
     4441 atcgatggag gcagggaatg cctcaatatg tgctcggaca tcgggaccgg ttggctaagg
     4501 ttcaggagca gttggcccat tcgccaggcc tttatctaac gggagcaggc atgcatggca
     4561 tcggtatccc tgattgtata cgggaaggca caaaggttgg aaagcaactc ctacaggatt
     4621 atgccgcgaa ccgtgcaatg aaaaattctg gaagcacaga atccaatgga aggggccaga
     4681 tcggataacg tggaagagca catggaagcg attgttcggg ctcatgcctg tgtcagtgga
     4741 aaagttcaag gagtcgggtt tcgagcattt gtgcaaatgc aggctaataa aatggtatta
     4801 catgggtggg tgcgaaaccg tgctgaagga ggagtcgaat tggaagttga agggcctgaa
     4861 gcatctgttc acacctttct tgaagccatt cacaaaggac ctcctctctc acaggttctt
     4921 caggtaacgg ttgactggaa agacccaaac aggcaaactg aagggtttca tgtccttcgt
     4981 acttctctgt aagggatggg ggccttgcgg atcggggaat tccgatctca aacactgaac
     5041 cattcacggg cgtcatcaag gttgagtcgt gatttcatga actacaaagc acatattcgc
     5101 gaggttccgg attttcctaa gccgggtatc cttttctatg atattacgcc cttattaaat
     5161 aatcctgttt gttttcgttc catcatcgat gagtgcattt gtcattatca ggatgccagt
     5221 attaccagag tggtgggaat tgaatcgcga ggatttattt tggggagccc attggcctat
     5281 cacctgaatg ccggatttgt tcctgtccgg aagcccggca aattgccggc cgatgtgttt
     5341 gaagtcaatt ataatttaga atatggctcg acggctttgg cgatccatcg tgatgcgatt
     5401 caaaagggag agcgagtgct tgtggttgat gatctccttg ccactggagg gacggccggg
     5461 gcgaccgtcc acctgattcg gcaattgggt ggcgagattg ccggcgtggt ttttttagtt
     5521 gaattactga agctcgatgg ccgaaacaaa ttgaacgggt gtgacattca ctccctgatt
     5581 acctatgagt gagtacatgg actatcgtat tgccgcatga tgcaacgggg aaggtgtcag
     5641 caatacatcc tcctgtcgtg tgagagaatg gttaactttg gaaggaagaa aggaatgtag
     5701 tatggccgaa agaccagtca agaaagaaac aaaaacaacg gcctccgccg gaacaaaaaa
     5761 aggtccggca aaaggcccat caaaagccac ttccccaact cccgcatcgc ggaagccggc
     5821 gaaagctcaa acggaagatc gtcatgtcgc gttaaaggaa attctcctgg cgaaacgaga
     5881 agcgctgatt caagaaatca aacaacagct tggacaatca gtgacagaag aacagcaacg
     5941 ccggctggaa gccgcgatgg atagcgggga ccaggcgctg gtggatttgg atcgcgaaat
     6001 ggggatttcg cttcaggaaa tgcgaaaccg tgaacgacaa ttgattgatg acgccttgga
     6061 tagtttagat gaaggtacct atggcgtgtg cgcggaatgt ggcggagaga tcaatgaaaa
     6121 gcgtttgcaa gcgcttccgt ttgcccgttt atgcgtcgag tgtaaatcta aacgtgaact
     6181 gatggaaaag attgaacgat ccgaacagcg gcaataatta tcaggtttgc cgcttctgtt
     6241 ctttcaccgt gtctcgctgg gattggcata caccaatcca atttggcatt ggcttttttc
     6301 gccttcaact gtcctcccac caacactgaa gcctgtgact gatcattcct gtaaaaaaaa
     6361 atccagatcc atttgatagg cgggagaaaa agggtgcaag gacggccttc gtaagctcat
     6421 gtcaaagatt gttgttctgg caagtggcgg gttggattcg acggtgacgg ccgccattgc
     6481 caaatcagag ggatatgagc tgtatttctt gactgtcgat tatgggcaac gccatcgaac
     6541 ggaaatcgaa tgtgcgcaaa aaatttctga atattttaag gtcaaagaac acaaaattat
     6601 ccaattggac ttgcgaacct ttggtggatc cgcgctaacc gatcagattc ccgtccccac
     6661 caatcgaacc ggacgggatc gtgaacagca aattcccgtc acctatgttc cggccagaaa
     6721 tacaattttc ttgagtctag cgctggccta tgccgaggtg ttagatgcct ccagcatatt
     6781 ttttggggcc aatattcgtg actattccgg ctaccccgac tgccgtccgg aatttattca
     6841 ggcgttcaag gaagtggctc gtcttggcac ccgaatggga ttggaaggcc aacaggtcga
     6901 cattctcgcc ccgctactct ttttgaccaa gcgtgagata atccaaaaag gacatgcctt
     6961 aggggtgcct tttcaatgga ccaatagttg ttataatcca ggtctacaaa accaaccgtg
     7021 cggccactgt gatagttgcg tgattcgtca agagggattt aaggaggcac aacttcctga
     7081 tccgctactc gccggatgaa tgtgggaccg tggcctcgtc tgattgcgaa ggagtctggt
     7141 ataaaggaac atggacatga acaagcattc taggcgaaca attctcaaaa gtgtggcagg
     7201 cgttctttgt tggtttgttg ttggatgttc tgagaatggg acgaatgccc gggaatcctc
     7261 cccgcctgca accatcgccc aagcatcggt agagaaagac ctgccaacag aattcaaaga
     7321 cggcgagaca aaattcaata cattttgttc tccctgccat ggagtgcagg ccgtgggaac
     7381 ggctcaaggt ccccctttgg tccataaaat ttatgagccg agtcaccatg cagatttcgc
     7441 gtttcagcgg gctgcggctc aaggagtcaa agcccaccat tggaagttcg ggaacatgcc
     7501 gaagatcgaa ggcgtgactg cagatgatgt cacgcagatt attggttacg tccggtggct
     7561 tcagcgccaa gctggaattt cctagagcga gtgttgaaaa ataccgccag cggcgttccc
     7621 tgcctttgcc ggagcggctt cgcgcaggca ggtcgccgct tgaacgtgct cacgtacttc
     7681 tccgtacgct ccgctcgccc aagcagctgc ggccttgctg gactagccat tttgaacact
     7741 cccttatttt tcctcgttgg ggtttccgtc attcatccaa ggcaaaatat gagaaatatt
     7801 caacagcctc ttagacatcg cgggttgtcg tccaaaacgt tcacggcata cttcgttcca
     7861 gttcattgac cagcccacgg atccgtgtaa atcccgcctg ccagaatgtc gtagagttaa
     7921 tatctacctg taggggtttg agcaggtctt gaggactggc cgacccacct cctgacagca
     7981 attgtaggta tcgtgggacg aacgagggcc cttctgtttg gtagcgttga aacaacgaga
     8041 ggactaatag gctgccaaag ctgtaggcgt agcaataaaa cggactggca aagatatgag
     8101 gaatggctag ccattcccat tgaaactcct tgtccacgtg taggcgaggc ccgaattgct
     8161 cacgaagaag ggtcaggtag gtttgagcga gagcatcaat ggtcgcgcct tgttgaatca
     8221 tgtgatgggc ttgccgttca aattgaacaa agtaggcctg tcgcataatt gtggcatacg
     8281 catcgtcgag ttgatcgacg agaagtcccg ccctgacctt agggtccatt tcctgttgga
     8341 gaagtaaatc ggagagcagg tgctcgccaa acacagaggc cgtttccgcc agagggagcg
     8401 acgaatgaaa cgtgaagatg gaatgctcac ttgccatcat cgcgtggacg gcatgaccca
     8461 gttcatgcgc taaggtcgag acatcacgag gctctccggt gaaatttacc aggacataag
     8521 gagtctgcgt ggggagcacg ctatagcaaa aagccccgcc cattttgccg ggcctaattg
     8581 cagcatcaag gtgattctct tcaagaactc gcttcgcccg ttgggccaat tccggggaaa
     8641 aggcttgata cgcttccatc actaactctt tcgccctggc aaaagaatat ttcgatttct
     8701 tcgccgcgaa gggagcatac agatcataac gttgaaactg ttttatcttc aggagccgtg
     8761 cctttaaccg gaaatattcc tggaaaatga cggcattctt tcggcaggtt ttgagcaggg
     8821 catcaacggc tttttcgggg acatcattcg tgacattgcg cacggccata ggagaagcgt
     8881 aatgccgcaa ggtgaggcct tcatttcccc aatcctgaac caggctctta tacatttctc
     8941 caaggacatc ccggtgttca ccataaacct tgaatagcgc gtggtaggct cctcgacgaa
     9001 ccgaagccaa gggatgccgg acatatccca tgagttgttc gcgggtcaga cgggtcgtcc
     9061 ggcctttcat tttcatggga aatgtcaaac tattcgtggt gacgccatac aatgtttcga
     9121 gagcctg
//
LOCUS       DATIAL010000457         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_835113, whole genome shotgun sequence.
ACCESSION   DATIAL010000457 DATIAL010000000
VERSION     DATIAL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835113"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /gene="lepA"
                     /locus_tag="VLA60_14210"
     CDS             complement(<1..1400)
                     /gene="lepA"
                     /locus_tag="VLA60_14210"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HSF10560.1"
                     /translation="MSKAFPQNNIRNFSIIAHIDHGKSTLADRFLEITGAVSQREARA
                     QYLDAMDLERERGITIKAHAVTTRFQSSDGQEYQFNLIDTPGHVDFAYEVSRSLAACE
                     GALLLIDATQGVEAQTIANAYLAISNNLVIIPVINKIDLASADVDGVKVQIRDVLGLS
                     GEEAFFVSAKDGRGVREVLEGVVRIIPPPAGSLSRPLKALIFDSWFDNYQGAVVLLRV
                     MDGEILPNMMIRLMSSGREFEVLEVGVFSPKRTKVDRLGPGEVGYICAGMKELSDTKI
                     GDTITDSRRPTETALPGYRDVKPVVFCGLYTTDNAKFEDLRDSLEKLRLNDSSFVYEP
                     ETSLALGFGFRCGFLGLLHMEIIRERLEREYGLDLISTAPTVVYRVYSINGEMIVIDN
                     PSRLPDPSQIERIEEPYIKATIITPERYIGNVLQLCQERRGIQVGLQYLDPTRSMLIY
                     ELPLNEIVLDFYDRLKS"
     gene            1485..>2224
                     /locus_tag="VLA60_14215"
     CDS             1485..>2224
                     /locus_tag="VLA60_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF10561.1"
                     /translation="MTVHRTRTQLEKDDCRYVWHPFTQMQEWERHPPLIIKRGHGSYV
                     YDMDGNAYLDATASIWVNVHGHRHPRIDQAIRRQLSQVAHTTLLGLSNPPAIQLAKML
                     IRLAPQGLQKVFYSDNGSTAVEIAAKMAIQYWQQCGTPQPRKIRFIHLGMSYHGDTVG
                     GVSLSGIELFRRPFSSLVFPTYDIEPPYCYRCPFGLHFPECRLACLDPLENLLRVRHQ
                     EIAGVILEPMVQGVAGIIPSPPGYLKRVR"
BASE COUNT          582 a          575 c          512 g          555 t
ORIGIN      
        1 gatttcaggc gatcataaaa gtctaacaca atttcattga gaggaagttc ataaattaac
       61 atggaccggg ttggatcaag atattgcaaa cccacttgaa tgccccgacg ctcctgacac
      121 agttggagaa cattaccgat atagcgttcc ggggtgatga tggtggcttt gatatatggc
      181 tcttcaattc gttcaatctg ggagggatct gggagtctgg aagggttgtc gatcacaatc
      241 atttccccat tgatcgaata cacccgatag acaacggtcg gggcggtact gatcagatcc
      301 agaccatatt cccgctccaa ccgctctcgg ataatctcca tatgtagaag ccctaaaaat
      361 ccacatcgaa atccaaaacc caaggcgaga gaggtttccg gttcatacac aaatgaggaa
      421 tcattgagcc gtaatttttc cagagaatca cgcagatcct caaatttggc attatccgtc
      481 gtgtataatc cacaaaaaac caccggcttc acatcacggt atcccggcaa agcggtttcc
      541 gtcggtcgcc gggagtcagt aatagtgtct ccgattttgg tatcggaaag ctctttcatc
      601 ccggcacaaa tatatcctac ttcccctggc ccaagtcgat cgaccttggt ccgtttcggt
      661 gaaaagaccc cgacttccag gacttcaaat tcacgtccgg aagacatgag gcggatcatc
      721 atattgggga gaatttctcc atccatgacg cgaagtaaca cgacggctcc ctggtaatta
      781 tcgaaccagg aatcaaaaat aagcgctttc aacggtcggg aaagagaacc ggcaggaggg
      841 ggaataatcc gaaccacacc ctcaagaact tctctcacgc cgcgaccatc tttggcactg
      901 acgaaaaagg cctcttcacc tgataatccc aacacatctc tgatttgaac tttgactcca
      961 tctacatcgg cactcgctaa atctatctta ttgatgacgg gaatgataac cagattattc
     1021 gaaatcgcta aataggcatt ggctatcgtt tgggcctcca ccccctgagt ggcatcgatc
     1081 agcaggagcg cgccttcaca ggcggccaga cttcgcgaga cctcataggc aaaatctaca
     1141 tgaccgggag tatcaataag gttaaactga tattcctgtc cgtccgagct ctggaatcgt
     1201 gtggtgaccg catgtgcctt aatagtgatg ccgcgttcac gttccaggtc catggcatcc
     1261 agatattgcg cacgcgcttc acgttgagaa acagcacccg tgatttcgag aaaccggtcg
     1321 gcgagagtgg atttgccgtg atcaatatgg gcaataatgg agaagttccg tatgttgttt
     1381 tgaggaaagg ctttactcat tcgaagtgtc cgtgattgta gtgagcgttg aaaaggttgt
     1441 caaaaattga tgaattccgc taggttattc ttctggaaag aaccatgacc gtccacagga
     1501 cccgcacaca attagaaaaa gacgattgcc ggtatgtatg gcatcccttt acccaaatgc
     1561 aggaatggga acggcacccc cctctcatta tcaaacgcgg acatggctcc tatgtctatg
     1621 atatggacgg caatgcctat ctcgatgcca ccgcatctat ctgggtgaac gtccatggac
     1681 atcggcatcc tcgcattgat caagctattc gccggcaact ctcccaggtg gctcatacca
     1741 cactgcttgg tctttctaac cctcctgcca ttcaattggc gaagatgctc atacgcctcg
     1801 ccccgcaagg tttacaaaaa gttttttatt ccgataacgg ctcgacggcc gtggaaattg
     1861 cagcaaaaat ggccattcag tattggcaac agtgtgggac tccacaaccg cggaaaattc
     1921 ggtttatcca cttgggcatg tcctaccatg gggatacggt aggaggagtg agcctcagcg
     1981 gaattgaact tttccgtagg cccttttcct cacttgtctt cccaacttat gacattgaac
     2041 cgccttattg ctatcgctgt ccctttggct tgcactttcc cgaatgcagg cttgcctgct
     2101 tggatcctct tgaaaacctt cttcgagtca ggcatcaaga aattgccgga gtgatccttg
     2161 agcccatggt acaaggagtc gctggaatca ttccttctcc acctggatac ctgaaacgtg
     2221 tccg
//
LOCUS       DATIAL010000458         5179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_82698, whole genome shotgun sequence.
ACCESSION   DATIAL010000458 DATIAL010000000
VERSION     DATIAL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82698"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VLA60_14220"
     CDS             complement(<1..507)
                     /locus_tag="VLA60_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HSF10562.1"
                     /translation="MGARQPTSSAKPPASSTGRAWSARKIAAATLQAIERSRGFSDDV
                     FDHITKDLVVAERDRHLAFELVYGVLRHYLTLDWRLDQVSRKPMVRLPLTVATTLRVA
                     AYQLLYLDRIPASAAVNEAVQLIQKQPGHNWSGFVNAVLRNLLRQPVPPMPDPAVDAT
                     QALSIRYAC"
     gene            complement(524..1549)
                     /gene="pdxA"
                     /locus_tag="VLA60_14225"
     CDS             complement(524..1549)
                     /gene="pdxA"
                     /locus_tag="VLA60_14225"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HSF10563.1"
                     /translation="MASTQTTKPLLAITMGDPAGIGPEIIVKALQLTKIWRICRPLVI
                     GSRTILEYTAQSLGTSLVLVPVSGHESSSDSRMFRRGSLPFLDPFSGTVRPVRLGRIT
                     ARSGDMAVRCIQTAVRLAQAGCVQGIVTAPINKHAMHLAGHTYPGHTEMLADLTKAKE
                     SGMMIVGGPLKILFATTHLALRDVPNVLTSTTILRAIRLAHQGLTRLFHIQKPRIGVA
                     GLNPHAGENGLFGDEEGRLIRPAIRQAKKQGIACTGPHPADTLFGKAVRGSFDGIVAL
                     YHDQGLIPLKTVAFGHCVNITVGLPIIRTSVDHGTAYDIAGQGMADPMSLVEAIEMAA
                     KLATHSP"
     gene            complement(1557..2786)
                     /gene="larC"
                     /locus_tag="VLA60_14230"
     CDS             complement(1557..2786)
                     /gene="larC"
                     /locus_tag="VLA60_14230"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HSF10564.1"
                     /translation="MNRHLHIDAFSGVSGDMFLGALVDTGVPLSALEKGLKGLGIKGY
                     RLREQQVIRNSIRATKVDVDIQKGFTKPLSLATIKKTLKNSRLSDTIRTQALHTFQLL
                     AEAEGRVHGQEPNKVHFHEVGVIDSLVDIVGTLLGFAHLNITTVSFSPINVGAGTIST
                     AHGLLPVPGPAVAHMAQGIPILSNGPAIEFTTPTGIALVKTLSQDCKPLPPLTPQTVG
                     YGAGTADPHGWPNVLRLFVGVQDSDSLNHTERIIQLETNIDDMNPQLYEAIMERLFEA
                     GALDVTLTPTIMKRSRPGTIVTVIAFSQDLQSLQSILFSETSTLGIRIQEMDRAILPR
                     SFQTIRLLGGSVRMKIAGLGQGRSKVTPEYRDCVTLAERTRQPVQTIIDLARRTYGTA
                     KTPSTPKTSPAKLAQRK"
     gene            2989..3213
                     /locus_tag="VLA60_14235"
     CDS             2989..3213
                     /locus_tag="VLA60_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10565.1"
                     /translation="MLTLSWPFGFPSRFAESGLGLNSALPSSRNRLYRSATPKAKEKI
                     IQQLWDLIGVKGKSLAQYEGCPQYRSAEDL"
     gene            3363..4214
                     /locus_tag="VLA60_14240"
     CDS             3363..4214
                     /locus_tag="VLA60_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10566.1"
                     /translation="MPRLFKLSLKTLQIFGLCLTIGLAFSQQAVAKPNKGPATSPVKI
                     SDVVVSPDPFILGKSGLKLTLLVELPSSLRGANVLEVSAMITSPTRRSMSFVAHRLLV
                     DELETKGKNLTIPVELVWDGKDQYHQLVADGSYFYELQAKLMEDQGNGPRTKIVSRRV
                     HGTLEALAYVGEVLPPLTPEPDIPEELEILREGESSEESGPNTEEGLKSSDDPVVPEE
                     EAPMDAAGALQPEEAGGSEADVQIGEPEEKDESIIPDMGESLTSESLNEPMPELPKSG
                     EDLPLPR"
     gene            complement(4180..4950)
                     /gene="larB"
                     /locus_tag="VLA60_14245"
     CDS             complement(4180..4950)
                     /gene="larB"
                     /locus_tag="VLA60_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HSF10567.1"
                     /translation="MNHDRLAALLQRVQGGHVSVPQAIRQLRTLPFEDLGFASVDHHR
                     SLRQGFPEVILCEGKTPAQITAIARKLLKAGGPFLATRVSPANARSLKRLARKAVYHE
                     MARMVSISHNKTDKQGLILIVTAGTSDIPVAEEAKVTAEVMGSRVQTLYDVGVAGLHR
                     LLDRQECLHQARVVVVVAGMDGVLPSVVGGLVDRPIVAVPTSQGYGANFGGLAPLLTM
                     LNACSSGIGVVNIDNGFGAGCLAHRININGEAEGLPQT"
     gene            complement(4947..>5179)
                     /locus_tag="VLA60_14250"
     CDS             complement(4947..>5179)
                     /locus_tag="VLA60_14250"
                     /inference="COORDINATES: protein motif:HMM:NF019122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10568.1"
                     /translation="STTRTVAEGVPTSGAAMALAERYQVDMPIVRGVFQMLFEGRNPR
                     HIVTDLMSRLAKGETDGLFSASTATFGPRAHS"
BASE COUNT         1199 a         1338 c         1470 g         1172 t
ORIGIN      
        1 acacgcatat cgaatggaga gggcctgggt ggcgtcgact gccggatcgg gcatgggcgg
       61 aacaggttgc cggagtagat tgcgcaatac ggcattcacg aaaccggacc aattgtggcc
      121 gggctgtttc tggatgagct gaacggcttc attcaccgcg gcggaagccg ggatacgatc
      181 gagatagagt aattgatagg cggcaacccg caacgttgtg gcgaccgtca gcggcaggcg
      241 gaccatgggc ttgcgggaga cctgatctaa tcgccaatcc agggtgaggt aatgccgtaa
      301 cactccatag accaactcaa acgccaggtg ccggtcgcgt tcggcgacca cgaggtcctt
      361 agtgatgtga tcgaacacgt catcgctaaa cccccgggat cgttcaattg cctgcaacgt
      421 ggcggcagcg atttttctcg cactccaggc acgaccagtc gaagatgccg gtggtttggc
      481 ggaggatgtc ggttgtcgcg ctcccatctt cacgccttgg caatcatggg gaatgtgtgg
      541 ccaacttggc agccatttca atggcctcaa caaggctcat gggatcggcc atgccttgcc
      601 cggcaatgtc ataggccgtg ccgtggtcaa cagaggtgcg aatgatcggt agacccaccg
      661 tgatattcac gcaatggccg aaggccaccg ttttgagtgg aatcaatccc tggtcatgat
      721 ataaggcgac aatgccatca aatgatcctc gtaccgcctt gccgaacaag gtatccgcag
      781 gatgaggacc cgtgcaggca atgccctgtt ttttggcttg ccgaatagcg ggtcgaatga
      841 gacggccttc ctcgtcgcca aacaggccat tctcgccggc atgagggttg aggcccgcca
      901 cgccgatgcg cggtttctga atgtggaaca agcgggtgag tccctggtga gccaatcgaa
      961 tggcccggag aattgtggtg gaggtcaaca cgttcgggac atcacggaga gccaggtgtg
     1021 tggtcgcaaa taaaattttc aagggacccc cgactatcat catccccgat tcttttgctt
     1081 tcgtcaggtc cgccaacatt tcggtatggc ccgggtacgt gtgccctgct aaatgcatcg
     1141 catgtttatt aatgggtgcc gtcacaatgc cctgtacgca accggcctgg gctaaccgga
     1201 cggctgtttg aatgcacctg actgccatat ccccggaccg tgccgtgatg cgtccaagcc
     1261 tgaccggacg gactgtcccg gaaaagggat ccaggaacgg gagagagccc cggcggaaca
     1321 ttcgagaatc ggaggatgat tcgtgcccgc tcaccggcac aagaaccagt gaggtcccta
     1381 aggattgtgc ggtgtattcc aggatggtcc ggctaccgat gaccaggggc cgacagattc
     1441 gccagatttt cgtcaactgg agagccttca caataatttc cggtccgatc ccggcaggat
     1501 cgcccatggt aatcgccaga agcggtttgg tcgtttgcgt ggaggccatg aatggattac
     1561 ttgcgctgtg ccaatttggc aggagaagtt ttgggcgtgg atggggtttt cgcagtgcca
     1621 tacgtccggc gtgcgaggtc gatgattgtt tgcacaggct gcctggtgcg ctcagccaag
     1681 gtgacacaat cccgatactc gggagttacc ttagaacggc cctgtccaag accggcaatc
     1741 ttcatgcgga cagacccccc caacagtcta attgtttgga aggagcgagg cagaatggct
     1801 cggtccattt cctggatccg tattcccagc gtcgaagttt cggaaaatag tattgactgc
     1861 agcgactgaa gatcctgtga gaacgcgatg actgtcacaa tcgtgccggg ccgactccgt
     1921 ttcatgatgg ttggggttaa tgtgacatca agagctccag cctcgaacag gcgctccatg
     1981 atcgcctcgt acaattgggg attcatatca tcaatgttcg tctcaagctg aatgatacgt
     2041 tctgtgtgat tcaaagaatc agaatcttga acaccaacaa ataaacgcaa cacattaggc
     2101 cagccatgag gatcggcggt acccgcccca taccccactg tttgtggtgt taagggtggg
     2161 agcggtttac aatcctggga gagagtttta accaacgcga tcccggtcgg ggtcgtgaat
     2221 tcaatggccg gtccattgga gaggatggga atgccctggg ccatgtgcgc caccgccggt
     2281 cccggcactg gaagcagccc atgcgccgtg gaaatagtac cggctcccac attgataggc
     2341 gaaaatgaca ccgtcgtgat gttcagatgc gcaaacccca gcagggttcc gacaatatcc
     2401 accagcgaat cgattacgcc cacctcatga aaatgtactt tattcggttc ttggccatgc
     2461 accctgcctt cggcttcagc cagcagttgg aaggtatgaa gagcctgcgt gcgaatggtg
     2521 tcggacaggc gactgttctt taaagttttt ttgatggtgg caagtgataa gggcttggta
     2581 aatccctttt ggatgtccac gtccaccttg gtggcccgga tgctgttgcg gatcacctgc
     2641 tgttctcgaa gccgatagcc ttttatgcct aagcctttga gccctttttc aagggcagac
     2701 aagggtacac cggtatctac gagggcgccg agaaacatgt ccccgctgac acctgaaaac
     2761 gcgtcaatat gaagatgtct gttcacgaga tgattcctca gtaaattcaa ggcttaaaac
     2821 gtatcatatc aaggaagtca aagcgactct gaagcgacca ataggaacgt atcgatcagt
     2881 gaaagaggaa atgagtgatg attaatcttt ttcaaatttt gcttggatcc ttgcgggttt
     2941 cgccccggcg gacgaggtcc ttttgttacg gcaaaaggac ccaaaaccat gttgaccctg
     3001 tcgtggccct tcgggttccc ttcgcggttc gccgaatccg gcctcggctt aaatagtgcg
     3061 ctgccgtcct cccggaatcg gctgtaccgc tcggccacgc caaaggccaa agaaaaaatt
     3121 attcaacaat tgtgggattt gattggtgtg aaggggaaat ctttggcaca atatgaagga
     3181 tgccctcaat accgatcagc tgaggatcta tgacctggtt ggagatcaca ccgtattgag
     3241 agacacttcg ttatactccg aatgacatgg ctgcccaggg attgcttcat tctggcgtcc
     3301 ctcgggattc atcgaccatt gactaatcga tggaattaca gtttttacaa ataaggaaac
     3361 agatgcctcg tttatttaaa ctttctctga aaacgctgca aatttttggc ttatgtctca
     3421 caataggttt agccttttcc caacaggctg ttgcaaaacc aaataaagga ccggcaactt
     3481 cccccgtgaa aatttctgat gtggtggttt ctcctgatcc ctttatcctt gggaaaagtg
     3541 ggttgaaatt gaccctgctc gtggagttgc cgtcatcgtt acgtggagcc aatgtgctgg
     3601 aagtctctgc catgattacg tcaccgacgc ggcgctccat gagttttgtc gcacatcgtc
     3661 tgttggtgga cgaactggag acgaaaggaa agaatctcac cattccagtg gagctggtct
     3721 gggatgggaa agatcaatac catcaactcg ttgctgatgg atcgtatttt tatgaacttc
     3781 aggccaagtt gatggaggat caggggaatg gtccacggac aaagatcgtg tcacgtcggg
     3841 ttcacgggac actcgaagcg ttggcgtatg tgggtgaagt gttgcctccc ctaactccgg
     3901 aaccggatat tcccgaagaa cttgagattc ttcgtgaggg agagagttct gaagagagtg
     3961 gcccaaacac ggaggaaggc ttgaagtctt ccgacgatcc ggtggttcca gaggaagagg
     4021 cccccatgga tgctgcgggt gctcttcagc cggaggaggc cggtggttcc gaggccgatg
     4081 ttcaaatcgg agaaccggag gagaaagacg agagcataat cccagacatg ggagagtcgc
     4141 taacatcgga atctttgaac gagccgatgc ctgagttacc taagtctggg gaagaccttc
     4201 cgcttccccg ttaatgttga tgcggtgggc gagacagcct gctccaaacc cgttatcgat
     4261 atttaccacg ccgattccgg atgagcaggc attcagcatc gttaagagag gggcaagccc
     4321 gccaaaattc gccccgtagc cctggctggt gggcacggcg acaatcgggc gatcaaccaa
     4381 ccctcccacc acgctgggca aaaccccatc cattcctgcg acgaccacca ccactcgtgc
     4441 ctggtgaagg cactcctggc gatccaagag ccgatgtaag cctgcgacgc caacatcata
     4501 gagcgtctgc acccggctgc ccatgacttc ggcggtgact ttggcttcct cggccactgg
     4561 gatatcggag gtgccggccg tgacaataag aatcaatccc tgcttgtctg tcttgttatg
     4621 ggaaatagag accattctgg ccatttcatg atacaccgcc ttgcgggcca atcgcttgag
     4681 gctgcgtgca ttagcgggcg agacccgtgt cgcaaggaac ggtccgccgg cctttaataa
     4741 ttttcgagcg atggctgtaa tttgtgcggg tgtttttcct tcgcacagga tcacttccgg
     4801 aaaaccctgt cgaagcgacc ggtgatgatc cactgaggca aacccgagat cttcaaacgg
     4861 gagggtgcgg agctgtcgaa tggcctgggg gacagaaacg tgaccgcctt gaacccgttg
     4921 aagcaaggcc gctaaccggt cgtgattcat gaatgagccc tggggccaaa ggttgcggtg
     4981 ctggcggaaa aaaggccatc ggtttcgcct ttggctaatc gggacatcag atctgtcaca
     5041 atatgtcggg gattgcgccc ttcaaataac atctgaaaca cgccgcgcac tatgggcata
     5101 tccacctgat aacgttcagc cagcgccatg gcagctccgc tggtgggaac gccctcagcc
     5161 acggttctgg tggttgagc
//
LOCUS       DATIAL010000459         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1049709, whole genome shotgun sequence.
ACCESSION   DATIAL010000459 DATIAL010000000
VERSION     DATIAL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049709"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /gene="nuoD"
                     /locus_tag="VLA60_14255"
     CDS             <1..1063
                     /gene="nuoD"
                     /locus_tag="VLA60_14255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HSF10569.1"
                     /translation="PDLGYLHRGVEKLAEGLTYMQMLPHTDRLDYVCAMTNNYAYVRA
                     VEKLINMTVPERAEYVRTIVAEMQRIVGHLFWLGTQALDIGAMTVFFWTFREREVLLD
                     LFEKLCGARLTLNYYRIGGVDADLTPEIISALRNFLKMFPDHIREYDTLLQKNRIWVA
                     RTKGVAILSAVDAINFGCTGPVLRGSGVAYDIRKLEPYGVYDRVEWEVPLGKDGDTYD
                     RYWVRMEEMWQSARIIEQCLDQLPDGPIMGIDAQVIPPPKPEVMRDMQSLIHHFIIFT
                     QGFKPPKGETYCATEVPKGELGFFIISDGSSRPYRLKIRSPSFIHMGAFDHMARGYLI
                     SDIVTIFGTYDIVMGECDR"
     gene            1190..1675
                     /locus_tag="VLA60_14260"
     CDS             1190..1675
                     /locus_tag="VLA60_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HSF10570.1"
                     /translation="MSRYPVKRSALLPLLNLAQREEGFVSESAMREIAKILDLTPPQV
                     FETVTFYTMFNLKPIGKFHLQVCKSLMCALVGSDDVVGWIQNKLGIGPGQTTPDKTFT
                     LSVVECLGSCGTGPMMQVNDDYYEQLTEEKTGRVLDDLKRDGTSTLKTGPFMYPQPHA
                     K"
     gene            1755..>2713
                     /locus_tag="VLA60_14265"
     CDS             1755..>2713
                     /locus_tag="VLA60_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HSF10571.1"
                     /translation="MAKYEKILLNNMEQPGYSGSLADYEQAGGYQALRTVLKDMQPDE
                     VIEVVKKSGLRGRGGAGFPTGVKWGFIPKDHPGPKYLCCNADESEPGTFKDRQLMERD
                     PHQMLEGMAITCFAIGAQTAYIYIRGEFRLGAKILERAIQEAYGAKYLGDNVLGSGLT
                     INVYVHQGAGAYICGEETALLESLEGKRGLPRFKPPFPATHGLYQKPTVVNNVETMAN
                     IPHIINRGGEWFAAIGSPPKSCGTRVFCLSGHVKLPGNYEVPMGITFRELIYELGGGM
                     RSDKPLKAFIPGGASAPFLTPEHLDVKMDFESVALAGSMLGSGG"
BASE COUNT          686 a          568 c          755 g          704 t
ORIGIN      
        1 tccggatctt ggatatttgc atcgaggagt cgaaaaattg gctgaaggac tgacctacat
       61 gcaaatgctg ccgcataccg atcgattgga ttatgtctgt gccatgacca ataactatgc
      121 gtatgtacgg gcagtggaaa aactcataaa catgaccgtt cccgaacggg cggaatatgt
      181 gcggacgatc gtcgccgaaa tgcaacgcat cgtcggtcac ttattctggt tgggaacaca
      241 ggcgttggat atcggtgcga tgacggtctt tttttggacc ttccgggaac gggaagtcct
      301 gttggattta tttgaaaaat tgtgtggggc tcgtctgact ttgaactact atcggattgg
      361 cggtgtggat gccgatctca ctccggaaat catttcggcc ctgcggaatt ttttaaaaat
      421 gtttcctgac cacattcggg aatacgatac tttattgcaa aaaaatagga tttgggtggc
      481 acggacgaaa ggagttgcga ttctttccgc agttgatgcc attaattttg ggtgcacagg
      541 accggttcta cgggggtctg gagtggccta cgatattcga aaactggagc cgtatggcgt
      601 gtatgatcgt gtggagtggg aagtgcctct cggcaaagat ggggatacct atgaccggta
      661 ttgggtcagg atggaggaaa tgtggcaaag cgcgcgcata attgagcaat gcctggatca
      721 attgcctgac ggacccatta tgggtatcga tgctcaagtg attccgcctc caaaacccga
      781 agtgatgcga gacatgcaaa gtctgattca ccatttcatt atttttacgc aaggctttaa
      841 gccacccaaa ggagaaacgt attgtgcaac ggaagtgccc aaaggcgaat tgggtttctt
      901 tattatcagt gacggaagtt ccaggcctta ccggttaaaa attcgctcgc cctcttttat
      961 ccatatgggc gcatttgatc atatggcccg tggatatttg atctccgata tcgtcacgat
     1021 tttcgggacc tatgacattg tcatgggaga gtgtgatcga taaccggaag ttgctgggag
     1081 ttctgtagaa gaaggcgaat tgctaaaata tcatcaaaat gattgttttc acctcagaaa
     1141 agaatgattg atgattttcg ataggtttac agacgaagtt aagactatta tgtcccgcta
     1201 tccggtgaag cgatctgcct tgttgccctt attgaacctg gctcagcgtg aagagggatt
     1261 tgtgagtgaa tcggctatgc gggagattgc caaaattctg gatttaaccc cgcctcaggt
     1321 gtttgagact gtcacgtttt atacgatgtt caatctcaag ccaatcggga aatttcatct
     1381 tcaagtctgc aaatcgctca tgtgcgcctt ggtcggatcg gatgatgtgg ttggttggat
     1441 tcagaacaag ctggggatcg gtccgggtca gaccacgccc gacaaaacct ttaccctcag
     1501 tgtggtggaa tgtctgggct cctgcgggac cggtccgatg atgcaagtga atgatgacta
     1561 ttatgagcaa ttaaccgaag aaaaaaccgg acgggtgttg gacgatttga aacgggacgg
     1621 aaccagtaca ttaaaaacag gacccttcat gtatcctcag ccccatgcta aatgattgga
     1681 tgatagatga taaactggcg aagtgcggtc gagtaattct ctgtatacgg caccatcctt
     1741 agcgaatatt ttttatggcc aaatacgaaa aaattttatt aaacaatatg gaacaacccg
     1801 gatatagcgg ctcgttggcg gactatgagc aggcgggtgg ttaccaggcg cttcgtacag
     1861 tcctcaagga tatgcagcct gatgaggtga ttgaggtggt gaaaaaatca gggcttcgag
     1921 ggcgcggagg agccggattt cccacagggg tgaaatgggg ctttatcccc aaagatcatc
     1981 cggggcccaa atatctgtgt tgcaatgcgg atgaaagcga gccgggaacc tttaaagacc
     2041 gtcaactcat ggaacgggat ccccatcaaa tgctggaagg gatggccatc acctgtttcg
     2101 ccattggtgc ccaaaccgcc tacatttata taagaggaga atttcgcctg ggggcgaaga
     2161 ttctggagcg ggctattcag gaagcctatg gagcaaagta tctgggtgac aatgtgctcg
     2221 gaagcggctt gaccatcaat gtttatgtcc accagggggc gggagcctat atctgtggtg
     2281 aagaaacggc ccttctcgaa tcgttggaag gcaaacgtgg tcttccacgc tttaagccgc
     2341 cgtttccggc gacgcatggt ttgtatcaaa aacccactgt ggtgaataat gttgaaacca
     2401 tggctaatat ccctcacatt atcaatcgag gcggggaatg gtttgctgca atcggctctc
     2461 cgcctaaaag ttgcggaacc cgggtgtttt gtctaagcgg gcatgtgaag ctgccgggta
     2521 attatgaggt tcccatgggc atcactttcc gggaattgat ttacgaattg ggtgggggga
     2581 tgcgatcgga taagcccctc aaggcgttta ttcccggggg agcatccgct ccatttctga
     2641 cacctgagca tttagatgtg aagatggatt ttgaatcggt ggccctggca ggatccatgt
     2701 tggggtctgg cgg
//
LOCUS       DATIAL010000460         4113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1049972, whole genome shotgun sequence.
ACCESSION   DATIAL010000460 DATIAL010000000
VERSION     DATIAL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049972"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /gene="hscA"
                     /locus_tag="VLA60_14270"
     CDS             <1..1507
                     /gene="hscA"
                     /locus_tag="VLA60_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HSF10572.1"
                     /translation="TFSPPQVSAMILKELKRRAEDYLKEDISKAVVTVPAYFNDSQRQ
                     ATKDAGMIAGLEILRIINEPTAASLAYGLQKKTQGLIAIYDLGGGTFDISILKLKDGI
                     FEVLATNGDTHLGGDDFDQLLVDVMVDDIFQTQGLKVGENPDLMQTIRLEAERVKIRL
                     SDETQARAVIELPNSTYEKVWTRESMETLTMGLVERTLAPCRLALKDAGLTASQIDEV
                     VLVGGNTRMPLVWRKVEELFGKKPHSELNPDEVVALGAAVQADILGGHTTDLLLLDVT
                     PLSLGIETMGGVMSALIRRNTTIPTSAKEMFTTYVDGQTSVDIHILQGERELVKDNRS
                     LARFQLKLPPLPAGVPRVEVNFLIDANGILQVTARDIRTGEAQSVQVKPSHGLTDDEV
                     EGMVRDSFQFATEDIKARQIIEARNEANAIIVATEKALGRAANLIHEKETESIRQILR
                     QLGEVKDGDDHRVIRAKIAEVEKTTHHLAEVLMDATLKEALESKKLSDVIK"
     gene            1578..1781
                     /gene="iscX"
                     /locus_tag="VLA60_14275"
     CDS             1578..1781
                     /gene="iscX"
                     /locus_tag="VLA60_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HSF10573.1"
                     /translation="MWGDTEDIAIRLQEEHPDLDPLTVRFTDLHAWVVALPEFKDDPK
                     TSNEKKLEAIQMAWYEEYQDARS"
     gene            complement(1823..>4113)
                     /locus_tag="VLA60_14280"
     CDS             complement(1823..>4113)
                     /locus_tag="VLA60_14280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HSF10574.1"
                     /translation="ILWIATRDLPTFDSFQDYHPSLVSRVYADNGELIGQFFIERRLY
                     TPIDKIPKAFTQAVIATEDTRFFDHPGLDIVGIGRAAWTNLKKGGRFQGASTITQQLA
                     RALFLSPERTYQRKIKELILAVKMEWVLTKEQILEMYLNQIYFGHGAYGVAAAALTYF
                     DKNVSDLSLPESAFLAGLPKAPNTYSPYRNPDLAKSRKELVLGRMVEAGYITNEEAQA
                     AMATTLAYHHQSIEPIAAYFLEEVRQHLVDRYGETLVYKGGLRIYTTLNIAMQKIAEE
                     AVRTGLRQLDKRQGWRGPIEHIAFSKDFTPPDTFPELQSPKAALVHGLYRALVTNVTK
                     QSAQILIGNTYKGSILFEDMRWAQRRLEKSGDVGTAVVRDKASPLQLLKVGDIIEVAP
                     KNGTVESGEFVLEQTPIVEGALIAMDPRTGAVQSMVGGYDFTRSQFNRAVIARRQPGS
                     AFKPLIYASALQQGLTPATLILDAPVVYEDEDLDRVWKPENYEKRFFGTITLREALRH
                     SRNAATVRLLEQIGVPQVVNIASNLGIRSPLSQDLSLALGSSSLTLQEITSAYGVFAN
                     QGLWLEPYLITHAENLNGEILEQHQFEPRQAMTKENAYLITNMLMDVIQSGTGRLAKS
                     IGRPLAGKTGTTNSYNDAWFVGYAPNLATGVWVGFDGVRTLGRLESGAHAALPIWTKF
                     VDQALLHSPVMTFPIPNDIQFAQIDTATGDLPSKTSRNISTEVFRKGTEPGKAAPQKA
                     NPMDFFEFDRLNSDSSNQLSPF"
BASE COUNT         1040 a          934 c         1105 g         1034 t
ORIGIN      
        1 aacattttct ccccctcaag tgtccgccat gattctgaaa gaattaaaac ggcgagccga
       61 ggattattta aaggaggaca tctctaaggc cgtggtcacc gtcccggcgt atttcaatga
      121 tagtcagcgg caagccacca aagatgcggg tatgattgcc ggattggaga tcctgcgtat
      181 cattaatgag cccacagccg cttccttagc gtatggcctg cagaaaaaga cacaaggtct
      241 gatcgcgatt tatgatttag gcggagggac gtttgatatc tctattctca aattaaaaga
      301 tggaatcttt gaagttctgg caacgaatgg ggatacccat ttagggggag atgatttcga
      361 tcaactgctc gtcgatgtga tggttgatga tatttttcaa actcaaggcc taaaagttgg
      421 cgagaatcca gatttaatgc aaaccattcg gttggaggcg gaacgtgtca aaatccggtt
      481 gtcggatgaa acccaagccc gggcggtgat agagcttccc aattcgacgt atgaaaaggt
      541 atggacgcgg gagagcatgg aaacgttaac gatgggtttg gtggaaagga cccttgcgcc
      601 ttgtcggctt gccctcaaag atgcaggttt aaccgccagt cagattgatg aagtggtcct
      661 ggtcggcggg aatacccgca tgccactggt ttggcgaaaa gtggaagaac tgtttggcaa
      721 aaagccgcac tctgaactga accctgacga agtcgtcgcg ttaggggcgg ctgttcaagc
      781 tgatatttta ggcgggcata ccacagacct gttgctactg gacgtgactc ccctgtcatt
      841 gggaattgaa acgatgggcg gggtcatgag tgcgctgatt cgtcgaaata ccacgattcc
      901 aaccagtgca aaggaaatgt tcacgaccta tgtcgatggg cagacctcgg tggatattca
      961 tatcctccaa ggagaacggg aattggttaa agataatcga agtttggccc gcttccaatt
     1021 aaagcttcca ccgttgccgg ctggggtccc tcgcgtggaa gtcaactttc tcattgacgc
     1081 caatgggatc ctacaggtga cggccagaga tatccggacc ggtgaggctc aatcggttca
     1141 ggtaaaacct tctcatggac tgaccgacga tgaggtggaa ggaatggttc gggattcttt
     1201 tcaatttgct accgaagaca tcaaagcccg tcagattatt gaagcacgga atgaagccaa
     1261 tgcgatcatc gtggcgaccg agaaagctct ggggcgtgca gcgaatttaa ttcatgaaaa
     1321 ggaaacggaa agcattcgtc agattttacg gcagcttggt gaggtgaaag acggggatga
     1381 tcatcgtgtc atacgtgcga aaattgctga ggttgaaaaa acgacgcatc atttggccga
     1441 agtgttgatg gatgccacgt tgaaagaagc tctcgaaagt aagaaattat ctgacgtcat
     1501 taaataatcc ctcaataggg agactttcga catgggcaaa acccattcaa ggggaagaaa
     1561 tcatggctca ggaattcatg tggggtgata ctgaagatat tgctattcgg ctccaggaag
     1621 agcatcccga ccttgatccg ttaacggtca ggtttacgga tttacatgcg tgggtggttg
     1681 cccttcctga atttaaggac gatcccaaaa cgtccaatga aaaaaaattg gaggccattc
     1741 aaatggcctg gtatgaagag tatcaggatg cccggtcctg agaaggcatg tgacggtgta
     1801 atcggcctgc tgaagtgttc tatcaaaatg gggaaagctg gttggatgaa tccgagttca
     1861 accggtcgaa ctcaaaaaaa tccatagggt tcgccttctg aggagccgct tttcctggtt
     1921 ccgttccttt acgaaaaact tccgtactga tgtttcgact ggtcttggac ggcaagtcac
     1981 ccgtcgcagt gtcgatctgg gcgaattgaa tgtcattagg aataggaaag gtcataactg
     2041 gtgagtgtag cagggcttga tcaacaaact tggtccaaat tggcagggct gcatgcgctc
     2101 cggattccaa tctgccgagg gttcgaaccc catcaaatcc cacccatact cctgtcgcca
     2161 aattcggggc atacccgaca aaccacgcat cgttataact atttgtggtt ccagtttttc
     2221 ctgccaatgg ccggccaata gatttggcga gtcttccggt tccgctttga atgacatcca
     2281 tgagcatatt ggtgatcaag taggcgtttt ccttggtcat ggcttgtcga ggttcgaact
     2341 ggtgttgttc taagatctcc ccattgaggt tctcggcatg tgtaatcaga taaggttcaa
     2401 gccacaaacc ctgattcgcg aagacgccat acgcggaggt gatttcttgg agggtcaggc
     2461 tggacgatcc caaggctaaa gagaggtcct ggctgagcgg acttcgaatt cccagattgc
     2521 tggcaatatt gacgacctgg ggcactccga tttgttcaag caacctgacg gttgcggcgt
     2581 tgcgggaatg gcgcagtgct tcacgaaggg taatggttcc aaaaaagcgt ttttcataat
     2641 tttccggctt ccatacccgg tctaaatctt cgtcttcata gacgacgggg gcatcaagaa
     2701 tcagggttgc cggtgtcagg ccttgttgaa gcgccgaagc ataaatgagt ggcttgaagg
     2761 cggagccggg ttgtcgacgg gcaatcactg cacgattaaa ctgacttcga gtaaagtcat
     2821 accctcccac cattgattgc acggcgcctg tcctcggatc catggcgatg agggcgcctt
     2881 ccacgattgg agtttgctct aagacaaatt cgcctgactc gacagtgcca tttttcgggg
     2941 cgacttcaat aatgtctccg accttcaaga gttggagcgg agaggcttta tcgcgaacga
     3001 ccgctgttcc cacatccccg cttttctcca atcgccgctg agcccatcgc atatcctcga
     3061 aaaggataga acctttatag gtatttccaa tcaagatttg agcagattgt ttggtcacgt
     3121 tagtcacaag cgcgcggtac agaccatgga caagagctgc ctttggactt tgtagctccg
     3181 gaaaggtatc gggaggcgtg aaatctttgg aaaaggcaat atgttcaatc ggtcctcgcc
     3241 agccttgtcg tttatctaat tggcggagtc ctgtacgtac cgcttcttcc gcgattttct
     3301 gcatggcaat gtttaaggtg gtgtagattc gcagcccacc cttatacacg agggtttctc
     3361 cgtaccgatc caccaagtgc tgacgcacct cttccaggaa ataggccgcg atcggttcta
     3421 tggattggtg gtggtaggct agggtcgtgg ccatggcggc ttgcgcctcc tcgttggtga
     3481 tatacccggc ctctaccata cgccctaaga ccaattcttt ccgcgattta gccagatctg
     3541 ggttccgata gggagaatag gtgtttggag ctttaggaag accagccaaa aatgctgatt
     3601 caggaagact caaatccgag acgtttttat caaaataggt taaggcggcg gcggccactc
     3661 cataggcacc gtgaccgaaa taaatttggt ttaagtacat ttctagaatt tgctctttgg
     3721 tcaacaccca ttccattttc actgcgagaa tgagctcttt gatttttcgc tgatacgttc
     3781 tttcgggaga aagaaaaaga gctctggcta actgttgggt aatggtgcta gcgccttgaa
     3841 accggccacc ttttttgagg ttggtccagg ccgcccggcc gatgccgaca atgtccagcc
     3901 cagggtgatc aaaaaaacgg gtatcctctg ttgcaatgac cgcttgggtg aaggctttgg
     3961 gaattttgtc gatgggcgta tacagacgac gttcaatgaa aaattgccca atgagttcac
     4021 cgttatctgc atagactcta gacactaaac tcggatggta gtcttgaaag gagtcaaatg
     4081 tgggaaggtc acgggtcgca atccagagaa tcc
//
LOCUS       DATIAL010000461         9937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1050012, whole genome shotgun sequence.
ACCESSION   DATIAL010000461 DATIAL010000000
VERSION     DATIAL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050012"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2433
                     /locus_tag="VLA60_14285"
     CDS             <1..2433
                     /locus_tag="VLA60_14285"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HSF10575.1"
                     /translation="GGGLAGLLALVGGGLSAKTSPEPVAHASAPPNEGWVKGDQSGSL
                     IGLDPEGKFIQAFPLKHTDVSIQVSGPLARAQVTQQFENPYPDKIEAVYTFPLPQHAA
                     VDAMMLTVGDRVVKGRIKKREEARAIYEAAKRRGNVAGLLDQERPNIFTQSVANILPG
                     EKITVTISYVEMLSYDDGIYSVVFPMVVGPRYIPGQPTAQVPSLGEGRAYDTDQVPDA
                     SHITPPVVKPGSRSGHDLSLSVTIDAGVPIQQVQSKTHDIVVEKSQTSQAEVHLKQEA
                     TIPNKDFILTYAVAGNDIHDAVLTHRNGEDGFFTLLLQPPDRVKTEEVTPKELVFVLD
                     TSGSMSGFPIEKAKETMRLAIEGLNPRDTFNLITFAGDTHILFPEPVPAIPANVRKAQ
                     AFLASRSSGGGTEMMKAIRAALEPSDSQEHVRIVCFMTDGYIGNDMAILSEVQNHPNA
                     RVFSFGVGSSVNRFLLDKMAEEGRGEVEYVGLNDDGTAAARRFHERVQHPLLTNLAVD
                     WNGLDVSDIYPKRLPDLFSAKPVVLTGRYTTPGKGTIRLRGKVSGRSVVRNIPVDLPG
                     FAPEHDVLASLWARTKIAELMAKDYTGIQQGSPRTAVREAITQLGLDYRLLTQFTSFV
                     AVEELLITEGGQSRRVEVPVELPEGVSYEGIFGSSDENAGSFRQHLMQPAAKMSRSFL
                     GGKVARESVASSPQTMVDRVEPERDDTRRGDYSSKMDSTLESLVEHVEKTIVLPQDVA
                     QYVTDGKVLLQVWLKDTNPETLEHLKKIGFELMAQPKTGRLIIGKLAFEKLKALAELS
                     MVRYITVQPTVM"
     gene            complement(3694..4071)
                     /locus_tag="VLA60_14290"
     CDS             complement(3694..4071)
                     /locus_tag="VLA60_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HSF10576.1"
                     /translation="MLDTCTFLWLISDDKALSPASREIFTDPSHVIFLSAISVWEILV
                     KYSLGRITLPTPVDQFIPQQRERHGISTLALEEAATGHLPKLPTLHKDPFDRMFICQA
                     IQHELTILTPDPLITQYAVRTAW"
     gene            complement(4077..4322)
                     /locus_tag="VLA60_14295"
     CDS             complement(4077..4322)
                     /locus_tag="VLA60_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HSF10577.1"
                     /translation="MIKLNIHEAKTHLSRYLDKLKKGETILLCKHNKPVAEIRGLPTP
                     LSRKRPIGLAKGQFTIPDSFFEPLPDELLKAFSGHSE"
     gene            complement(4482..5405)
                     /locus_tag="VLA60_14300"
     CDS             complement(4482..5405)
                     /locus_tag="VLA60_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10578.1"
                     /translation="MSTLAIFVIPFVVILGIVLVSLTLPFWRRDPSPVSFGLDDDRAQ
                     ELLDLQIERETVVRSLQDLELELSQGRMDPSDYERLKATDERRLLSLLDRLDTFHDVV
                     HDETSEHQPATSKRINWVPTIASGLVVLLASIGIYSALQFKAAQKLMAVEAQMGGGPN
                     PMEMVAKLEKRLKDNPDDLQGQIMAGRSYMALNRMPEAKKAWEKALELDPKQHEAHFN
                     LGVIQIETRQIDDPEIFKQALAHFDMVLADLPNQPAVNWYRGLALWYLNRRQETDAAW
                     TLAAQNMEPGTKDIEFVKDALIKLRAGEVPF"
     gene            complement(5422..5886)
                     /locus_tag="VLA60_14305"
     CDS             complement(5422..5886)
                     /locus_tag="VLA60_14305"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HSF10579.1"
                     /translation="MSIRLTWCLAALLMIPLPLSAAVQDETDVDIQVREIAKTLRCTV
                     CQTENIWESGAPLAQQMRGVIRDRIALGHSTEEIRAYFLSRYGDYILMEPPKHGVNWL
                     IWVAPFLLLVIGGFFLYKEVTHWVRDTPTPPSQPEPPLDDEARKRIERELKS"
     gene            complement(5886..6449)
                     /locus_tag="VLA60_14310"
     CDS             complement(5886..6449)
                     /locus_tag="VLA60_14310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HSF10580.1"
                     /translation="MNKGWQVTLILCLIGLFALFYQGLWGDPRHIPTVLIETDAPNFE
                     GPDVETGQTISLDQFKGKVVLLNFWASWCYECKVEHKSILQLHQLFKDNPDFVMLGVN
                     YQDELPDAQKYLKEYGSTFSHVRDLKGQLAIDYGVYGVPETFVIDQQGKIRHKWVGPI
                     TGEVYTNITQKVIAPLLNAQPTTTTTS"
     gene            complement(6446..8395)
                     /locus_tag="VLA60_14315"
     CDS             complement(6446..8395)
                     /locus_tag="VLA60_14315"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HSF10581.1"
                     /translation="MIIEIGHFSTIVALVLSVFGMASAVLALQTRNPDWARSGRIAIT
                     LIFVLLGVGMLSLVYAFIIRDFSVLYVANNSNSKLPFFYTVAAVWGGHEGSLLLWVFI
                     LSVFSTLAVWLHWRTQPAIMPYLIGVESAIIFGFLCLIIFLSNPFERLLPVPLDGKDL
                     NPLLQDPAMVMHPPMLYMGYVGFSIPFSFAMAALFSGRLGEEWIKVTQRWSMFAWMCL
                     TTGILLGGYWAYYELGWGGYWGWDPVENASFMPWLVGTAYLHSVLVQEKRKMFKVWNL
                     FLIIVTFSLSLIGTFLVRSGVLTSVHSFATDPERGLYILIFVGTVIGIAFGALILRSN
                     KLKSQVEMDSLISRESIFLFNNLFFLVAAATVFLGTLYPLILETLQDAKVTVGPPYYN
                     AVFMPIALGLLFLMGIGPYIPWRKASVASLKKSFSTPLLAGAVIVALLFVTGIHNLYA
                     LAGGYVVTFAGMAIVLDFGKISQLYAQRNGSNIFQGSLAAFTANQRRYASMLTHIGVL
                     VLVVGIIASTVYQTEKVVSMRVGDEFEIGDYRMKLAKIHEVAGGNWNAQEGVFQVFKG
                     EDLITELRPQKRIYSATQTPTTESAIYATNMGHVFLTMPEVSEDGVAVARGVLNPLVL
                     WVWGGGVIMGLGVILNILRPRKKEE"
     gene            complement(8414..8869)
                     /gene="ccmE"
                     /locus_tag="VLA60_14320"
     CDS             complement(8414..8869)
                     /gene="ccmE"
                     /locus_tag="VLA60_14320"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSF10582.1"
                     /translation="MGTKNKKIAIIVSILLVAGSLGYLAFGNFGENIVYFVTPSEVKA
                     FTAEAYSKKVRVGGMVVKGSLKTQPEPVGLTFELTDGAATIPVAFQGIPPDLFKEGQG
                     AVVEGQWRDGRTFHSTMIMAKHSEDYMPMELKRAGVELPKKDFMKTLSP"
     gene            complement(8853..9365)
                     /locus_tag="VLA60_14325"
     CDS             complement(8853..9365)
                     /locus_tag="VLA60_14325"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HSF10583.1"
                     /translation="MTGLYTRWALILGVTLLLAILTYQHYQQHLSTLSMGTLLSSPPV
                     TQPVRIQGMVKSGTLQGDVEHGQATFEFVDGEASLAVEYQGPPLENIRELKTLVLIGL
                     WDSQAQIFRAQDTALINNFGFVAAAYLISVMALGWMVFAMSQRVMVLFKEIKESKLYE
                     PEADTLGHKE"
     gene            complement(9362..>9937)
                     /gene="ccsA"
                     /locus_tag="VLA60_14330"
     CDS             complement(9362..>9937)
                     /gene="ccsA"
                     /locus_tag="VLA60_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HSF10584.1"
                     /translation="HVPLAHTSTLAYSVLFFGSIWYLWKRDPLVDNMCQASASICALF
                     TSLALITGSIWAKPTWNTWWTWDPRLVSFTVLLLILGGYIMLRRLVDDRVQGGRYAAI
                     LAIVGGIDLPIIKFSVEWWRTLHQPMSFSTRGVSISEDILVPLTLMSIGCWLLFAYMV
                     MVRTQILYLTDLLHAKKGRFLSQTHFSQTTP"
BASE COUNT         2446 a         2358 c         2686 g         2447 t
ORIGIN      
        1 ggaggagggc tggctgggtt gttggctctt gtgggagggg gactcagtgc gaaaacttcc
       61 cctgagccgg tagcccatgc cagtgctcct cccaatgaag ggtgggtgaa gggagatcaa
      121 tccggaagtc tgatcgggct ggatccggaa gggaaattca tccaagcttt tcccctcaag
      181 catacggatg tgtccataca ggtcagcggg ccactcgccc gggctcaggt cacccagcag
      241 ttcgaaaatc cctatcccga taagattgaa gctgtctaca cctttcccct cccacagcat
      301 gcggcggtgg atgcgatgat gcttacggtt ggagaccggg tagtgaaagg gaggattaaa
      361 aagcgagaag aggcgagagc catttatgaa gcggcgaaac ggcgaggcaa tgtggcgggg
      421 ctcttggatc aggaacggcc aaatattttc acacagtcgg tcgcgaatat tctgccgggg
      481 gagaagatta cggtgacgat cagttacgtg gagatgctgt cctatgacga tggaatctat
      541 tcggtggtgt ttccgatggt ggttggtccc cgctacattc caggacaacc cacagcccag
      601 gtcccatcgc tcggcgaggg gcgggcctat gacaccgatc aagtcccgga tgcctcccat
      661 atcacgccgc ctgttgtgaa gccgggcagt cggtcgggac atgacctgtc gttgtccgtc
      721 acaattgacg ctggtgtacc cattcaacag gtccaatcca aaacgcatga cattgttgtg
      781 gaaaaatcac agacttctca ggcggaagtt cacctcaaac aggaagcgac tattcccaac
      841 aaagatttta tcctgaccta tgctgtggcg gggaatgata ttcacgatgc ggtcctgacg
      901 catcgaaacg gagaagatgg gttttttacc ttgcttctcc agccgcctga ccgggtgaag
      961 accgaggagg tcacgcccaa ggaattggtt tttgtgttgg atacctccgg gtccatgtcg
     1021 ggttttccca tcgaaaaggc caaagaaact atgcgcctgg ccatcgaggg attgaatccg
     1081 cgcgatacgt ttaatcttat tacctttgca ggtgataccc atattctgtt tcccgaacct
     1141 gttcccgcaa taccggcgaa tgtccggaag gcccaggcgt ttctggcctc acggtccagc
     1201 ggcggtggaa cggaaatgat gaaggccatt cgtgcggcgc tggaaccctc cgattcgcaa
     1261 gaacatgttc gcattgtgtg tttcatgacg gatgggtata tcggcaatga tatggccatt
     1321 ctgagcgaag tgcagaacca tcctaatgcc cgcgtttttt cattcggagt tggtagcagt
     1381 gtcaatcggt ttttgttgga caagatggcc gaggagggtc gtggtgaagt ggagtatgtg
     1441 ggcttgaatg acgatggcac ggctgcggcg cgccgttttc atgagcgggt ccaacatccg
     1501 ttgctcacga atttggccgt ggactggaat gggctggatg tgtcggatat ctatccaaag
     1561 cgactccctg atttgtttag tgcgaaaccg gtggtgctga caggccggta tacgactccg
     1621 ggcaagggca cgatacggtt gcgtggcaag gtgtcagggc gttcagtggt gcggaatatt
     1681 cccgtcgatc tcccaggttt tgctccagag catgatgtgc tggcttcttt gtgggcgcgg
     1741 accaaaattg cggagctgat ggcgaaggat tacaccggga ttcaacaagg aagtccccgg
     1801 acggcggtgc gggaggccat tacgcaatta gggttggatt atcgactgtt gacccagttc
     1861 acctcatttg tcgcggtgga agagttgctc atcacggaag gtggtcaatc tcgtcgtgta
     1921 gaagtgccgg tggaactacc ggaaggcgtg agctacgaag gcatttttgg aagcagtgac
     1981 gaaaacgcag ggagttttcg tcaacacctg atgcaaccgg ccgcgaaaat gagtcgatct
     2041 tttctgggag gaaaggttgc ccgggaatcc gtggcctcca gcccacagac tatggtggat
     2101 cgcgttgaac cggaaaggga tgatactcgt cggggagatt actccagcaa gatggattct
     2161 acactcgaat cattggtgga acatgtggag aagaccatcg tgttgcctca ggacgtggcc
     2221 caatatgtga ctgatgggaa ggtattgctt caagtctggt tgaaagatac caatcctgag
     2281 accttggagc atttaaaaaa aataggcttt gaattgatgg ctcaacccaa gacgggacgt
     2341 ttaattattg gaaagctggc ttttgaaaag ttaaaagcgc tggccgaatt gtccatggtg
     2401 cggtatatca cggtgcaacc cacagtaatg taaaaccgag gtggttagaa tcaggcggct
     2461 cgggtattac atccgagccg cctttttttt gcgtctcctg catgttctta gcaaaaatcc
     2521 atcttttcta cgctgctgag tgagtaaaat ggacacccgc cacaaaaggc tataggactg
     2581 aaagccgatg tttttaaagt ctcctgaaaa tctcacaact ggcttgggct aaagcgcatt
     2641 cttattgtgg gcattgcgtt atttttctat tcaataaagg tggttgatgc ttccaggtct
     2701 gatggcaaag aacactttgc cgacaagtga aagcataata gtaagcgttt gagtgcgttt
     2761 ttcgtgtgat aactatgacc gaattatctc agagcattcc tctgaaaaaa cttcccgtcc
     2821 tggcgacgat tcgggaagcc ctcatggttt catggcagca tcgggctata ttattccgtt
     2881 gtataattgg gtgtacctta ttcgccggcg ccttttgttc cctgcaagag tacatcgttg
     2941 tagagtgcgt ttgggccaat aaggactgga attctttatc aggtggaatt tccttcttta
     3001 ttctttcggc gtttcccacc ttaatgattt ctgctctgtt ttctgttttt tgccatcgct
     3061 tagttttagc aaatgggaag ggaaaatctt cgagtcggat tgcctttggt aaacgagagt
     3121 ggcgattttt cggctggaat ttaactctgt gtttggccgc aattttcatt gtccttcttg
     3181 caatattagt tcgttacctt ctctggaaat gttggagaat ttaaaagaga attatggagg
     3241 ccttgtttta ataaaaacct tcttggataa ctattcattg aatattttgt tctatggagg
     3301 attttcctat cttcttgcac cctattgttt ggtactacca gcaactgcag tcgatcttaa
     3361 gccatctttg gcatggtcat cggaactaac taaagggaat gaattgagac tggcactatt
     3421 atttggaggc ttgcccctta ttttcggatt tttatactgc tctccctcgc tattgacttg
     3481 gttggaaatt gatcaaatgt tgattgtgaa acatgtgttc aggccatttc ttgtgtattt
     3541 ggttactccg atcatcgtaa tcgtgttatc catcgcgttt cgtgaactcc ccaatgggac
     3601 tccatcttcc tcccattccg aaaaagcctc tatagcttaa agcccctttg cgcttttgtc
     3661 ctgttgccag gaacacattg gtttcaaagg catctaccaa gcggttcgga cggcatattg
     3721 ggtaatgagt ggatccgggg tcaggatggt cagttcgtgt tggatagcct ggcaaatgaa
     3781 catgcggtca aatgggtctt tatgaagtgt agggagtttg ggcaggtgtc cggttgcagc
     3841 ttcttccaac gcgagtgtgc tgatgccatg gcgctcccgc tgttgaggga tgaattgatc
     3901 cactggtgtg ggaagggtaa tcctacccag agagtatttc accagaattt cccagacgga
     3961 aatggcactc agaaagatta catgcgaggg atcagtaaat atttcccgac ttgctggcga
     4021 gagcgccttg tcgtctgaaa tgagccacag aaatgtgcag gtatccagca gaacctttac
     4081 tcggaatgtc cggaaaacgc tttgagcagt tcgtcgggta ggggttcgaa aaagctatcg
     4141 ggaatggtga attgaccttt ggctaagcca ataggtcgtt tgcgtgaaag gggcgttggc
     4201 aaccctctga tttcggccac gggtttattg tgcttacaca aaaggatcgt ttctcccttt
     4261 ttaagtttgt ccagataacg ggacaagtgt gtcttggctt cgtgaatgtt gagtttaatc
     4321 atagttcaaa attaaactat aaaatgaact aagtcaattc tgtccaattg gtcgaacgct
     4381 ttgttgtgat ggggatgtcg acaattttaa aatcgttttg cacgtaatcc atgtgctact
     4441 catcgcccac ttaaactcac gtgtatttaa atcagaaata actaaaacgg aacttcaccg
     4501 gcgcggagtt tgatgagggc gtctttaaca aactcgatgt ccttggtgcc gggctccatg
     4561 ttttgtgcgg cgagggtcca ggcggcatcg gtttcctggc ggcgattcaa ataccacaag
     4621 gctaaacccc gataccaatt gacggcgggt tgattgggta ggtctgccag aaccatgtca
     4681 aaatgtgcga gagcctgttt aaatatttcc ggatcgtcaa tttgacgcgt ttcgatctgg
     4741 atcactccta aattgaagtg ggcttcatgc tgtttcgggt ctagctctaa ggctttttcc
     4801 caggcctttt tggcttcagg cattcgattt aaggccatgt aggatcgtcc ggccatgatt
     4861 tggccctgca ggtcatcggg attgtctttg aggcgttttt ccagtttggc gaccatctcc
     4921 atcggattcg ggcctccgcc catttgggct tcgacggcca tcaatttttg tgcggccttg
     4981 aactgaagcg cgctgtaaat gccgatggag gccagtaaga ccaccaagcc agaggcgatg
     5041 gtcggaaccc aattgattcg tttcgaggtt gccggttggt gttcgctggt ttcgtcgtga
     5101 actacgtcat ggaaggtgtc cagccggtct aacagactga gtagacggcg ttcgtctgtg
     5161 gccttcaatc gttcatagtc ggacgggtcc atcctcccct gtgagagttc caattcgagg
     5221 tcctggagag aacggaccac ggtttctcgt tcgatttgaa ggtcaagaag ttcctgagcc
     5281 cggtcatcgt ccaggccaaa ggaaacgggt gaaggatccc gccgccaaaa tggaagggtc
     5341 agggagacaa gcacgatccc caggatgacc acaaacggaa tcacgaaaat ggcgagcgtc
     5401 gacatagggg attgagacta ttcaggactt gagttcacgc tcgatacgtt ttcgagcctc
     5461 atcgtccaat ggcggttcag gttggctggg cggagtgggt gtatcacgga cccagtgagt
     5521 cacttccttg tagagaaaaa atcccccgat gaccagtagg agaaacggag ccacccagat
     5581 gagccaattg acaccatgct ttgggggttc catgagaata tagtcaccat agcggctgag
     5641 gaaataggca cgaatttctt ccgtgctgtg ccccagggcg atccgatcac gaatcactcc
     5701 ccgcatctgt tgggccagtg gcgcgcccga ctcccaaatg ttttccgttt ggcagacggt
     5761 gcagcggagg gttttggcaa tttcccggac ctgaatgtcg acgtccgtct cgtcctgcac
     5821 ggcagcggac agcggaagag gaatcattaa gagagccgca aggcaccagg taagtcggat
     5881 ggacattagg atgtcgtggt cgtggtcggc tgagcattga gcaacggggc aatgactttt
     5941 tgtgtgatgt tggtatacac ctcaccggta atcgggccga cccatttatg tcggattttt
     6001 ccctgttgat caatcacaaa agtttcgggt accccataga ccccgtaatc aatggccaat
     6061 tgtcctttga ggtcacggac atgggaaaag gttgagccgt attcctttaa atacttttgg
     6121 gcatcaggca gttcgtcttg ataattcacg cccagcatca cgaagtcggg attatcttta
     6181 aagagttgat gaagctgtaa gatgcttttg tgctccacct tgcattcata gcaccatgag
     6241 gcccagaaat tgagcaggac cactttccct ttgaattgat ccaaagagat cgtttgtccg
     6301 gtttcaacat ccggtccttc aaagttgggt gcatcggtct caataaggac agtgggaatg
     6361 tgtcgtggat ctccccagag accctgataa aacaaggcga acaatcctat aaggcagagg
     6421 atgagggtca cttgccagcc tttgttcatt cttctttctt tcgcgggcgg agaatattca
     6481 aaatcacgcc gaggcccatg atgacaccgc ctccccatac ccacagaacc agcggattga
     6541 gcacgccacg tgcgacggcc acaccgtcct cggacacctc cggcatagtc agaaagacgt
     6601 gccccatgtt ggtggcataa atggccgatt cagtggtagg cgtttgggtg gccgaataga
     6661 ttcgcttttg gggccggagt tccgtaatca ggtcctctcc cttaaatact tgaaagaccc
     6721 cttcctgcgc attccagttc ccgccggcca cttcatgaat ttttgccagt ttcatgcgat
     6781 agtccccgat ttcgaattcg tctcccacgc gcatcgagac gactttttcc gtttgataca
     6841 cggtggaggc gatgatgccg acgactaaga cgaggactcc gatatgggtg agcatgctgg
     6901 catagcgtcg ttggttggcg gtaaaggccg ccagacttcc ctggaagata ttgctcccgt
     6961 tccgctgtgc atacagttgc gaaatttttc caaagtccag gacgatcgcc atgccggcga
     7021 atgtcaccac ataccctcct gccagggcat agaggttgtg aatgccggtt acaaacagca
     7081 gggcgacgat gaccgctcct gctaaaagtg gtgtggagaa actttttttc aggctcgcca
     7141 ccgacgcttt ccgccatggg atatagggcc cgatacccat gagaaataac aggccaaggg
     7201 caatgggcat aaataccgca ttgtaatagg gcggcccgac ggtcaccttg gcgtcctgca
     7261 acgtttcgag gattaaagga tagagggtgc ccagaaagac cgtggcggca gccaccagaa
     7321 agaataaatt attaaagagg aagattgatt cacgggaaat caatgaatcc atttcgacct
     7381 gcgattttaa tttattggat ctcaggatta atgcgccgaa ggcaattccg atgaccgtgc
     7441 cgacgaatat caaaatatac agaccacgct caggatccgt ggcaaaggaa tggacggacg
     7501 taagcacgcc gcttctcacg agaaaggtcc cgatgagaga caaggaaaag gtgacgatga
     7561 tgaggaataa gttccagact ttgaacatct tccgcttttc ctgcaccaga acggaatgca
     7621 ggtaggcggt gcccaccagc caaggcataa acgaagcgtt ctcaacggga tcccagcccc
     7681 aatacccacc ccaacccagt tcgtagtagg cccaatagcc cccgagcaga atgccggtcg
     7741 tcaggcacat ccaggcaaac atgctccatc gctgcgtgac cttgatccat tcttctccca
     7801 atcgcccaga aaataaggcc gccatggcaa aggaaaatgg aatggaaaag cccacataac
     7861 ccatatagag catgggaggg tgcatgacca tcgccgggtc ttgcagcaac ggattcaaat
     7921 ccttgccatc gagggggacg ggtaagagac gttcaaacgg attggagaga aagataatta
     7981 aacataaaaa tccgaaaatg atggctgatt ccacgccaat caaatacggc atgatggcgg
     8041 gctgggtgcg ccaatgcagc cagacggcca gggtgctaaa gacggaaaga atgaagaccc
     8101 agagcaggag cgagccttca tgcccgcccc agactgccgc gacagtatag aaaaacggga
     8161 gtttcgaatt ggaattgttg gcaacataga gaaccgagaa gtcccgtatg ataaaggcat
     8221 agaccaatga gagcatgccc acgcccagca gaacgaagat gagggtgata gctatgcgcc
     8281 cggaacgggc ccaatcgggg ttccgggttt ggagggccaa tacggcgctg gccatgccaa
     8341 agactgacag caccagggca acaatggtcg aaaaatgtcc aatttcaata atcatagaga
     8401 atggcgaaca gggttagggc gaaagggttt tcatgaaatc ttttttggga agttccacac
     8461 ccgctcgttt gagttccatg ggcatgtagt cctcggaatg cttggccatg atcatcgtgg
     8521 aatgaaaagt ccggccgtcc cgccattggc cttccaccac cgccccttgt ccctccttaa
     8581 acaggtccgg gggaataccc tgaaacgcga caggaatagt ggcggcacca tctgtaagct
     8641 caaaggtcag acctaccggc tcgggttggg ttttaaggct acctttaacc accataccac
     8701 ccacacgaac ttttttgctg taggcttcgg cggtaaaggc ttttacctcg gagggagtca
     8761 cgaagtacac gatattttca ccgaaattcc caaatgcgag atagccgagc gaaccggcaa
     8821 ccaggaggat gctgacaatg atggcgattt ttttattctt tgtgcccaag ggtgtctgcc
     8881 tctggttcgt aaagtttgga ttctttgatc tctttaaata aaaccatgac tctttggctc
     8941 atggcaaata ccatccaccc caaggccatg acggaaatca ggtaggcggc ggccacaaat
     9001 ccaaaattat taattaaggc ggtgtcctgc gcgcgaaaaa tctgtgcctg gctgtcccag
     9061 agcccgatga gaaccagggt tttcaattcg cgaatgtttt ccaggggggg accttggtat
     9121 tccacagcaa gactggcttc cccatccaca aattcaaatg tggcctgacc atgttccacg
     9181 tcaccctgca aggtaccact tttgaccatc ccttgtatcc tgaccggttg ggttaccgga
     9241 ggactgctca aaagggttcc catggacaat gtggataagt gttgctggta gtgctgatag
     9301 gtcaggatgg cgagaaggag ggttacccct agaatcaacg cccagcgtgt atataacccg
     9361 gtcatggagt cgtctgagaa aaatgggtct gactgagaaa gcgacctttt ttggcgtgca
     9421 ataagtcagt caagtacaaa atttgagtgc ggaccatcac catataggca aacaataacc
     9481 aacagccgat gctcattaac gtcaagggta ccagaatgtc ttctgaaatg ctgactcctc
     9541 tggtggagaa cgacatgggt tggtggagtg ttctccacca ttccaccgaa aattttatga
     9601 tgggcagatc gatgcctccg acaatagcca gaattgccgc atagcggccg ccctgcaccc
     9661 ggtcatccac gaggcgccgg agcatgatgt acccccctaa aatcaggagg agcaccgtaa
     9721 acgagacgag ccgtgggtcc catgtccacc aggtgttcca tgtgggtttt gcccagatcg
     9781 aaccggtgat gagggcaagt gaggtaaaga gggcacaaat gctcgccgag gcctggcaca
     9841 tattatccac aagcggatcc cgtttccaca aataccagat gctcccgaaa aataatacgg
     9901 aataggccaa cgtgcttgtg tgagccaagg gcacatg
//
LOCUS       DATIAL010000462         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_407716, whole genome shotgun sequence.
ACCESSION   DATIAL010000462 DATIAL010000000
VERSION     DATIAL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407716"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VLA60_14335"
     CDS             <1..183
                     /locus_tag="VLA60_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10585.1"
                     /translation="LIGCGAVGPPIPPEEVGIEAKVRKQQQEQARKEGLTEEEQSTTP
                     VEEAVELPALYPIGTR"
     gene            283..2160
                     /locus_tag="VLA60_14340"
     CDS             283..2160
                     /locus_tag="VLA60_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF10586.1"
                     /translation="MDTTTTLIGTRPKLLLLDPYPRNNPYRMTASERRSIWFPKLSLP
                     VIAAYTPPTWDIELVDEAVTDINFDTHCDLVGLSIMTCYAPRAYEIADEFRRRGKKVV
                     LGGVHPTYCPDEALRHCDAIVCGEAEDLWPQVVADIEAGTLKQIYRMDQFPALSDYKP
                     PRIELLSPDSYMTRLCSFTTRGCHFDCEFCSVSPFNGKTTRRRPVPEVIEELKHAKEW
                     LRSDIVERMTRGSLLNALTTSLKIWVGLEEGSIVAFVDDLHNSHRAYCRELWQALKSL
                     NIKWGCQSTLFLGDDSEMVKLAADSGCVSVFVGMESLSDDSLDETNKGFNQVRKFENQ
                     IKMFHDHGIMVNPGIVFGFDNDDESVFETAVEFLTRNKVELAYFNVLTPLPGTALFER
                     YTREGRIFDQDWAKYDGKHVVYKPKRMTPEQLQEGFFWANHQFYSWPCIWHRVAHTSQ
                     RLIPRLDMNWEFRKLICRTTPKGTLSPLAKVLKNLQAKLPTFETQQLIPNALIPQASS
                     GFKPQDLCLKVKARRHDAFAALFIDLEGTLDHLNARELLDRIKQAARDAKMDIVVNFE
                     NLKQATPKAIHTLLDGEILQAITPHAKLRYRKLKEAFQSSVAEISLGDLDLLDEGAQH
                     A"
     gene            2153..>2931
                     /gene="lysA"
                     /locus_tag="VLA60_14345"
     CDS             2153..>2931
                     /gene="lysA"
                     /locus_tag="VLA60_14345"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HSF10587.1"
                     /translation="MHDFHYQGGELFCENVPIQKIAEEVGTPCYIYSHRTLIRHFRAF
                     DQAFQAIPHIVAFAMKSNSNLTVLRLLAKEGSGADIVSGGELFRALHAGIPPNKIVFA
                     GVGKSKEEIRYALESEILMFNVESPGELQKINEVAGAMGLRARVALRINPDIDPQTHP
                     YISTGLKKSKFGIEADRALEEFDAAGNLSHIEVVGVHSHIGSQLTQVTPFVDALKKAI
                     ALIQTLQAKDVHIQYLNIGGGLGITYSDETPPHPKELATAIS"
BASE COUNT          791 a          699 c          694 g          747 t
ORIGIN      
        1 ctgattggtt gcggtgcggt tggtcctccc attccaccag aagaagtcgg aattgaggcc
       61 aaagtgcgga agcagcaaca ggaacaggcc agaaaagaag gtctgaccga ggaggaacaa
      121 tcgactacgc cggtggagga agcagtggaa ttacctgcat tgtatcccat aggtacccga
      181 tgaggtttaa atgcaatacc tcaaatttgt ccgtagcggt cctcacgggg tagaatcaag
      241 gttatttcat ttgaacccat ctgtttgcgg aggataaaaa gaatggatac tacgaccacg
      301 ctgataggaa ctcgtcccaa actgcttttg ctcgacccct acccccggaa taatccgtat
      361 cgtatgacgg caagcgaacg ccggtcgatc tggtttccca aactcagttt gcccgtcatc
      421 gcggcttata ctccgcctac atgggacata gaactcgtgg acgaagccgt cacggatatt
      481 aattttgata ctcattgtga tttggtcgga ttgtccatca tgacatgtta tgcacctcgc
      541 gcgtatgaaa tcgccgatga atttcgtaga cgtgggaaaa aggtggttct cggaggggta
      601 caccccacct attgccctga tgaagcactt cgccattgcg atgctattgt ctgcggtgag
      661 gcagaagacc tatggcctca agtcgttgcc gacatcgaag ctgggacctt gaaacaaatc
      721 tatcggatgg atcaatttcc tgctctgagt gattacaaac ctccacgcat tgaattgctt
      781 agccctgact cctatatgac ccggttgtgc agcttcacga cacgaggatg ccattttgat
      841 tgcgagtttt gcagcgtctc accgtttaat ggcaaaacca cccgacgtcg tcccgtcccc
      901 gaagtcatcg aagaactcaa acacgcgaaa gaatggttac gttcggatat tgtcgaacgc
      961 atgacccgcg gctcactcct caatgcctta acaacttcct tgaaaatatg ggttgggttg
     1021 gaagaaggtt caattgtcgc ctttgtggat gatttgcata acagtcatcg agcctattgc
     1081 cgtgagttat ggcaggcact gaaatctctc aacattaaat ggggttgtca atccacgctc
     1141 ttcctcggcg atgattctga aatggtgaaa ttggcagcgg atagtggttg cgtctctgta
     1201 tttgtcggaa tggagtcctt gtcggatgat tccctggatg aaaccaacaa agggttcaat
     1261 caagttcgga agtttgaaaa tcaaattaaa atgtttcacg accacggcat tatggtcaat
     1321 cccggtatcg tatttggttt tgataatgat gatgaatcgg tgttcgagac tgccgtggaa
     1381 ttcttaacgc gtaataaagt ggaattggca tactttaatg tgttgactcc cctaccggga
     1441 accgcactgt tcgaacgtta tacacgggaa ggccggatct ttgatcagga ctgggcaaag
     1501 tatgacggaa aacacgtggt atacaaaccc aaacgcatga cccctgaaca gttacaggaa
     1561 gggttttttt gggcaaacca tcaattttat tcctggccat gcatttggca tcgtgtcgca
     1621 cataccagtc aacggctcat cccccggctc gacatgaatt gggaattccg aaaattgatt
     1681 tgtcgtacca cacctaaagg caccctttcc cccttagcca aggtcttaaa aaacctgcag
     1741 gccaaacttc cgacctttga aacccagcaa cttattccta acgctcttat cccccaagcg
     1801 tcttctggtt ttaaaccaca ggacctctgc ctgaaagtga aggctcgtcg gcacgatgcc
     1861 ttcgcggcat tgttcattga tctcgaggga accttggacc atttaaatgc cagggaatta
     1921 ttagacagga ttaaacaagc cgcacgggat gccaagatgg atatcgtggt caattttgaa
     1981 aatttaaagc aagccactcc caaagccatt cacacattgt tggatggaga aatcctgcaa
     2041 gccattaccc cccacgccaa attacggtat cgaaaattaa aagaggcctt tcaatcttct
     2101 gtagcggaaa tctcacttgg ggatttggat ttgcttgatg aaggtgccca acatgcatga
     2161 tttccattat caagggggag agctattttg tgagaatgtc ccaattcaaa agattgccga
     2221 ggaggtagga accccttgtt atatatacag ccaccgcaca ttaattcgac attttcgggc
     2281 ctttgatcag gcatttcagg ccattcctca tatcgtggca tttgccatga aatcgaattc
     2341 caacctgacc gttctgcggc ttctggccaa ggaggggagt ggagcagata ttgtgtcggg
     2401 cggagaactt tttcgcgcac tacatgccgg aatacctccc aacaagatcg tatttgccgg
     2461 agttggcaaa tccaaggaag aaattcgata tgcattagaa tcggagattc tgatgttcaa
     2521 tgtcgaatct cctggcgaac tccaaaagat caatgaagtg gctggcgcca tggggctgcg
     2581 cgcgagggtg gctttacgta tcaatcctga cattgatcca caaacacatc cctacatttc
     2641 caccggatta aaaaaaagca aatttggaat tgaggctgat cgggccttgg aagaatttga
     2701 cgcggcaggg aatctatctc atattgaagt ggtaggggtc cattcgcaca taggttccca
     2761 actaacccaa gtcaccccat ttgttgatgc cttaaaaaaa gctattgcat taattcagac
     2821 gcttcaagca aaagacgttc atattcaata cctgaacatt ggcgggggac ttggaattac
     2881 ctattcagac gaaacccctc cgcaccccaa agagctggct acggccattt c
//
LOCUS       DATIAL010000463         3089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_728564, whole genome shotgun sequence.
ACCESSION   DATIAL010000463 DATIAL010000000
VERSION     DATIAL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728564"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VLA60_14350"
     CDS             complement(<1..506)
                     /locus_tag="VLA60_14350"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HSF10588.1"
                     /translation="MRLTTATTANNRPSFREHYLLQGLCAWYVLLWTILAIDPLDRQD
                     WLLENILALGLIVVLIATYRHFPLSDLSYMLLALFLSLHAIGAHYTYSKVPLGFWIQD
                     LLNLERNHFDRIAHFSFGFLLVYPLRELFLRRVRVRGFWAYYLPVSGVLALSGFFEIV
                     ESWIARLV"
     gene            complement(475..1140)
                     /locus_tag="VLA60_14355"
     CDS             complement(475..1140)
                     /locus_tag="VLA60_14355"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HSF10589.1"
                     /translation="MKHVLLAGLLLWYVAFFFFMARNPVDPHSWGLASILPVVFVGFL
                     ASTYQHLSFSSVSYVLMTFFLTLHTIGSHYTYEQVPFGIWLEQILELPRNHFDRLVHF
                     CFGLLFAYPLFELFGKIGGFSVRLRSYMVLMTLVGLGGLWEILESWVARIVRPELGPA
                     FLGAQGDLWDAQKDMAATLYGVLICLAIMLVARRLIISPAVMPTERIMELQHAPHHCD
                     YRQ"
     gene            complement(1340..1798)
                     /locus_tag="VLA60_14360"
     CDS             complement(1340..1798)
                     /locus_tag="VLA60_14360"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HSF10590.1"
                     /translation="MKTPASIKGHPLHPMLIPLPLGLWIFSWIADLIWLSGWGSQLWQ
                     EIAFYTLGGGLVGAFLAAIPGLMDYLSLTDSHVKKIGTRHLTVNLIIIMICVINVWMR
                     ADGISYEGAFALSTLNIMLLGLSGWLGGELVYKHGVAVDTPPVLRTTGKR"
     gene            complement(1994..>3089)
                     /locus_tag="VLA60_14365"
     CDS             complement(1994..>3089)
                     /locus_tag="VLA60_14365"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HSF10591.1"
                     /translation="VIAGYHWFTDWGRDTMISLEGLMLATGRHADAGYTLRLFAYYIR
                     DGLLPNLFPEGGKEGLYHTADATLWFFHAIDRYVEATGDMATLEILLPRLLTIASAHL
                     QGTRFGIHVDPEDDLLRQGEESYQLTWMDAKVDEWVVTPRRGKAVEINALWYNALCLL
                     ADWVHQLRGNSEADPWRVHAEKTRSSFNRRFWYDKGGYLFDVVDGEEGDDTACRPNQL
                     LAFSLKHPVLDRRQWEPVLDVVRQRLLTPVGLRSLSPDHPHYHATYDGDIHARDAAYH
                     QGTVWAWLIGPFIDCWLKVHPDDQAGARQLLSGFDEHLNEACIGSISEIFDAEPPFTP
                     RGCIAQAWSVAEVLRCWVKTAPVKTNTPHS"
BASE COUNT          890 a          792 c          775 g          632 t
ORIGIN      
        1 ctgaccaatc gcgcaatcca ggattcgaca atttcaaaaa acccactcaa ggccagaacg
       61 ccactgacag gcaaataata cgcccagaaa cccctgacac ggacccgacg aagaaataat
      121 tcacgaagcg gatataccag aagaaaacca aatgaaaaat gggcgatgcg atcaaagtga
      181 ttccgttcca ggtttaacaa gtcctgaatc caaaaaccga gaggaacttt ggaatatgtg
      241 taatgagcac caatggcatg gagggacaga aaaagagcga gtaacatata ggagagatct
      301 gataggggaa aatggcgata ggtggcgata agcaccacaa tcagtcccaa cgctaaaata
      361 ttttcgagaa gccaatcctg gcggtcgagt ggatcaatgg caagaatcgt ccataaaagc
      421 acataccagg cacagagtcc ttggaggaga taatgttcgc gaaatgatgg tctgttattg
      481 gcggtagtcg cagtggtgag gcgcatgctg gagttccatt attctttcgg tgggcataac
      541 ggcaggagag atgatgagtc gccgggcgac tagcatgata gccaggcaga tcaggacgcc
      601 atataaagtc gccgccatat ctttctgcgc atcccataaa tctccctgtg ctccaagaaa
      661 agccggaccc aattccggcc tgacaattcg cgctacccag gattcaagga tttcccacag
      721 tccgcctagc cctaccaacg tcatcagcac catatatgag cggagccgga cagaaaatcc
      781 tccgattttt ccgaaaagtt cgaataatgg atacgcgaaa agcaacccga aacaaaaatg
      841 aaccagtcgg tcaaaatgat tgcggggtaa ctcaagaatt tgttccagcc atatcccgaa
      901 tgggacctgt tcataggtgt aatgcgatcc gatcgtgtgt aaagtcagaa aaaatgtcat
      961 caggacatac gacacactgg aaaatgatag gtgttgatag gtcgaagcca aaaaacccac
     1021 gaaaacaaca ggaagtatac tggcaaggcc ccaactgtga ggatcaacag gattcctcgc
     1081 catgaagaaa aagaatgcga cgtaccagag aagtaacccg gccaacaaca cgtgtttcat
     1141 acatctccct caggaaattg aagaaaaatt tgattcgcca gcttgtgtat tgtctccgaa
     1201 caatttccaa atgacaagtc aagggagctg agtctgataa tgattgtgag aaaaatggat
     1261 ttgaggaaaa gaggagagga ttgggaggta cggaacgctg tcttggctta tgaagacgag
     1321 aaattcccat gaataaagac taccgtttgc ccgtcgttct gaggacggga ggagtatcga
     1381 cggctactcc gtgtttatac actaactctc cgcctaacca gccggacaat cccagcagca
     1441 tgatattgag cgtggagaga gcgaaggctc cttcataaga gatgccgtcg gcgcgcatcc
     1501 aaacattgat gacgcatatc ataataatga tgaggttaac ggtgagatgc cgggtcccga
     1561 tttttttcac gtgagagtct gtcagggaaa gataatccat taatcctgga atcgcagcga
     1621 ggaaggcccc caccaagccg ccccccaggg tgtagaaagc tatttcctgc cacaactgac
     1681 ttccccatcc actcaaccag atcagatcag caatccacga aaaaatccag agtccaagcg
     1741 gaagcgggat aagcataggg tgaaggggat ggcctttgat actagcaggg gtcttcataa
     1801 ctccatcttc ctcttcagat aaagcgcata tccaaacagc gttggatact catcttctga
     1861 caaatagtgt aaacccgatc cctcattgcg cggcactctc taaaaccctg gacagagaga
     1921 atttccgaaa tggtccagga tgcagtgttg gacaactgca ggattgtgga ccagtttcgg
     1981 gggagccggt tcgttacgaa tgcggagtat tcgttttaac cggcgcagtc ttgacccagc
     2041 agcggaggac ttccgccacg ctccaggcct gagcaataca tccccgtggg gtaaacggtg
     2101 gctcggcatc aaagatttcg ctgatgctgc cgatacaggc ttcattgaga tgctcgtcga
     2161 aacccgacag caattgacgc gcaccagcct gatcgtccgg atgaactttc agccagcagt
     2221 cgataaacgg tccaataagc catgcccaca ccgtcccctg atgataggcc gcatcgcgtg
     2281 catgaatatc gccgtcatag gtggcatggt agtgaggatg atcgggcgaa agcgatcgca
     2341 agccgactgg cgtcaacaaa cgctgccgaa ccacgtccag aaccggctcc cattgccgcc
     2401 gatccagaac aggatgcttg agcgagaagg cgagcagttg attaggccgg caggccgtat
     2461 catcgccctc ctccccatcc acgacatcga ataaatatcc gcccttgtcg taccagaagc
     2521 ggcgattgaa cgatgagcgg gtcttctcgg cgtgcacccg ccatggatcc gcttcgctgt
     2581 tcccccgtaa ttgatgaacc cagtcggcca gcaggcataa ggcgttgtac caaagggcgt
     2641 taatttctac agcttttcca cgccgtggtg tcacgaccca ctcatcgact ttggcatcca
     2701 tccaggttaa ttggtagctt tcttctccct gccgcaacaa atcatcctcc ggatcgacgt
     2761 gaattccgaa tcgggttccc tgcagatgag ccgaggcaat cgtcaaaaga cgcgggagca
     2821 ggatttcaag tgtcgccatg tctcccgtgg cttccacata ccggtcaatg gcatgaaaaa
     2881 accaaagcgt cgcatcggcc gtgtgataga gcccctcttt gccgccttca ggaaacagat
     2941 tcggcagcaa accatcccgg atgtaatagg caaataatct caaggtatag ccggcatcgg
     3001 catgacgtcc ggtcgccaac atgaggcctt ccagactgat catggtgtca cggccccaat
     3061 cggtgaacca atgatagccg gcaatgact
//
LOCUS       DATIAL010000464         7975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1373598, whole genome shotgun sequence.
ACCESSION   DATIAL010000464 DATIAL010000000
VERSION     DATIAL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1373598"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..816
                     /gene="tmk"
                     /locus_tag="VLA60_14370"
     CDS             19..816
                     /gene="tmk"
                     /locus_tag="VLA60_14370"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HSF10592.1"
                     /translation="MKPRTHAKGKQGLSSGLFITFEGTEGSGKTTQCHRLARALRTQG
                     YQVLETREPGGTPLSEAIRRLLLSHSKTKSNKEFITPECETALILAARSQHVAHVIRP
                     ALSKGMIVLCDRFFDSTVAYQGYGRHRDITFLKHSHQFATEGLTPHLTFLLDLPTQEG
                     LARRQQARHQNRLDQESLDFHQRVRQGFIALAKQHPQRIQKLDARQSPEILNTLIASM
                     MARLIQSLPSTGLAHVTPQSSRIIKRVQTKGTPQHGVSRSRRASTPN"
     gene            776..1807
                     /gene="holB"
                     /locus_tag="VLA60_14375"
     CDS             776..1807
                     /gene="holB"
                     /locus_tag="VLA60_14375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HSF10593.1"
                     /translation="MAFRDLVGHQRPIKWLQTAVSTNHLGHAYLFHGEPTIGKRLTAI
                     ALAQYLHCEVPQIAPTPDACGHCRSCHQIAQATHPDFLLVQPEDTQKQNPKITIDQIR
                     DIEHLVIYRPLLGSHKICLIDQADSMTTEAANALLKTLEDPPDHCLFLLITNRQEHLL
                     ATIRSRCISLRFAPLPSASIYEFLKERTDREDQENRLIAAFAEGRLGSALACDPTELK
                     VKIRQYWALLFGEDTASPSQVMDMSEGLVKSNQVQEAIHWFWAGLRDLLLLTLDPSLS
                     PTLYQDQETALRELSLQITPSSLLDLIHELNQLERGQQRNLNVQIGLEQFFFHLHDQL
                     EFSHSPGLA"
     gene            1894..3879
                     /gene="metG"
                     /locus_tag="VLA60_14380"
     CDS             1894..3879
                     /gene="metG"
                     /locus_tag="VLA60_14380"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HSF10594.1"
                     /translation="MSNTFYVTTPIYYVNDVPHIGHAYTTIAADVLARFYRLTGDEVF
                     FLTGLDEHGQKVQQAAAKAGIDPQAHCDLLTPKFKNLWSQLHISNNGFIRTTDLQHQA
                     IVQRYLQALYDKGLIYQAEYTGWYCTFDERFWTEKDVENGLCPDCLRPVEQVSERNYF
                     YRMSRFQNRLKEHIQTNPNFIQPDTRRNEVLGFLQKPLEDLSISRPKSRLSWGIELPF
                     DRECVAYVWFDALVNYLSALEYVAPTPSTTKFWPASLHLVGKDILTTHAVYWSTMLMG
                     LELPLPQSIFAHGWWTVNGEKMSKSRGNVVDPYAVVQEFGADAFRYFLLREVPFGQDG
                     DFSITAFGTRYNADLANDLGNLLSRTLTMLANFSNNTVPQPNAKHETDQDRQLQHAAS
                     SLFPSIDTHLRHLEFNRALETMWGLVQSANQYIDKTAPWMLAKDPDHTPRLHTVLFHL
                     TEVLRFLTVAVHPFMPQIAETMKTRLGLSMDFSTPVLQEFPRWGAYLFTGQTSKGASL
                     FPRKDSRPSDSQKSPEQKMPIHPNRTHMETPQPVPSIQPQSPPADVIQIGIADFQKVQ
                     LKVAKVLTAERVPKSEKLLKLQVDIGTEQRQIVAGIGKKYAPEEMVGRTIVVVANLKP
                     AKLMGIESQGMVLAAGDQEVLELLNMSQEIPAGTRIK"
     gene            complement(4088..5488)
                     /locus_tag="VLA60_14385"
     CDS             complement(4088..5488)
                     /locus_tag="VLA60_14385"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HSF10595.1"
                     /translation="MLKRHSEFLKNLLFLSDLVVICVCWVAAYFIRFSVPLFPITKGI
                     PPIDPYLWLLFPIVAVWGICFYSFNLYRPRRMGSHLAEFVDIAKANTLSILILVALTF
                     FSKPFEFSRLVIMYFWLLNLVVLGFSRMVFREVLRVFRRMGYNQRQVVIIGAGKLGQR
                     VGDTLKMHPELGLQVRGYLTRNAEKVGQVLDGTPVIGTFAQAADILTSQVDIVFLCLP
                     PEVECEAEGLMKILSATTVGVKIIPSIYEFVTLRAEAEMFEGLPIITLQGSPLYGWNL
                     LLKRMVDICGAAVALVVASPIALMIAVLIKLTSPGPVFYRQTRVGLDGKSFNIVKIRT
                     MRMDAEEKTGPVWAKAHDPRRTPIGTFLRRTSLDELPQFWNVLKGEMSIVGPRPERPE
                     FIEKFRAQIPQYNLRHTMKAGITGWAQINGLRGNTSWEKRLAYDMYYIEHWSLWLDVK
                     IMIMTLWKGLIHREAY"
     gene            complement(5482..6666)
                     /locus_tag="VLA60_14390"
     CDS             complement(5482..6666)
                     /locus_tag="VLA60_14390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF10596.1"
                     /translation="MKVALVHDWLTGMRGGERCLEALCELLPDAPIYTLFHVKGSVSE
                     TIERHLIIPSFLNRVPFAKSRYRYLLPFFPSAIQRLEFHQYDLVISSSHCVAKGIRVP
                     RETCHISYVHTPMRYIWDGFDTYFVKPGIWNFGRLGMGLFRTRLQQWDMESNAHVSCF
                     IANSHNVAERIARQYGRAACVVYPPVDWQTFHVSHRHEGFYLMVTAFAPYKKVDLAIA
                     AANELGLPLKIIGQGQDEKRLRRMAGPSIEFLGWQPDHRVREFYSRCLAVLFPGEEDF
                     GIVPLEAMAAGKPVIAYGKGGALETIVPLNPKHKPGENHLERGLHGSGSSSVPTGVFH
                     YEQSVQAIIESIQLFTQHLTDFNPDAIRAHVEPFDRSHFKQRMQQVIMSRYREFRRTP
                     PC"
     gene            complement(6675..7757)
                     /locus_tag="VLA60_14395"
     CDS             complement(6675..7757)
                     /locus_tag="VLA60_14395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF10597.1"
                     /translation="MRVLQIVADGKPGGGTTHVLQIFKGLRHAVSFQLITEEQSYLMK
                     EAGALGIPCQGLRFFISRLNPAIPFQLRALVMAFHPDLVHVHGGRAGFFFAVSLLKVP
                     MVYTVHGYHFVEKSPIIRRMALMAERWNLRRACHSIFVSKYDVVLAKKFQLLSGSAKR
                     SVIYPGLSLKNLPPPLPQALLHIGFIGRLEPQKDPLLFVEMMEFLPEYSATIVGDGAL
                     APMIKQEIARRGLGGRVHMVGELSHGEALEILATFSVVVLTSRWEGLPVLVLESMGMG
                     VPVVSMNVSGLEEIIHDEVNGMLVEKRRGEDLAERVKIITNNEDLRISLIKKAQETIQ
                     EKFSIETMMASILKIYQEMSASHAGH"
BASE COUNT         2181 a         2165 c         1778 g         1851 t
ORIGIN      
        1 cgatcagagt aaatttccat gaaaccgaga acccacgcaa agggaaaaca gggcctgtct
       61 tcagggttat tcatcacctt cgaaggcaca gaaggctctg gaaagaccac acaatgccac
      121 cgattggcac gagcacttcg aactcagggc taccaggtat tagaaacccg tgagcctgga
      181 ggaacaccat tgtctgaggc cattcgtcgc cttctgcttt cccactccaa aacgaaatcc
      241 aacaaggaat tcattactcc agaatgcgaa acagctctaa ttcttgctgc ccggtcccaa
      301 catgtggctc atgtgattcg gccagccctg tcaaaaggta tgattgtgct gtgtgaccgg
      361 ttttttgatt caactgtggc ctaccaggga tacggacggc accgtgatat cacttttctc
      421 aagcattccc atcagtttgc cacggaagga ctgactccac acctcacgtt tttactcgat
      481 ctcccgaccc aagaagggtt ggctaggcgc caacaggccc gacaccaaaa tcgacttgac
      541 caggaatcct tggatttcca tcaacgagta cgtcaaggct tcatcgctct cgctaaacag
      601 catccccaac gcattcaaaa attggatgcc agacaatccc ccgagatctt gaatactctt
      661 atagcatcca tgatggcacg cctcattcag tcgttgccat cgacaggcct ggctcacgtc
      721 actcctcaat ccagccgcat tatcaagcga gtccagacga aagggacccc tcagcatggc
      781 gtttcgagat ctcgtcgggc atcaacgccc aattaaatgg ctccaaacag cggtctccac
      841 caatcatctg ggtcatgcct atctgtttca tggagaaccg accattggga aacgacttac
      901 cgctattgcg cttgcccaat atctccattg cgaggtccca caaatcgcac caacacccga
      961 tgcctgcggc cactgccgat catgtcatca aatcgcccaa gccacccatc ccgacttttt
     1021 gttggttcaa cctgaagata cgcaaaagca gaatcccaaa attaccattg atcaaatacg
     1081 cgacatcgaa cacctggtca tttatcgtcc tctcctgggg tcacataaga tttgcctcat
     1141 tgatcaggcc gactcaatga ccaccgaagc agccaacgcc ttgctcaaga cattggagga
     1201 tccgccagac cattgccttt ttctcctgat caccaaccgc caggagcacc ttttggcgac
     1261 gattcggtct cgatgcattt cgttgcgatt tgccccgctt ccctctgcca gcatttatga
     1321 attcctgaag gaacggacgg acagggaaga ccaggaaaac cggttaatcg cggcatttgc
     1381 ggaaggccgg ttgggttcag ctttggcttg tgaccccaca gaattaaagg tgaaaatccg
     1441 tcagtactgg gcgttgttat ttggggaaga tactgcgtcg ccttcacagg tgatggatat
     1501 gagtgaaggg ctggtcaaat cgaaccaagt acaggaagcg attcattggt tttgggcagg
     1561 gctcagggac ttgctattgc tgaccctgga tccttccttg tctcccacct tgtaccagga
     1621 tcaagaaacg gcgcttcgcg agttgtctct acagatcacc ccctcttcac ttctggactt
     1681 aatccatgaa ctcaaccaac ttgagcgagg gcaacaacga aatcttaatg tgcaaatcgg
     1741 attggaacaa tttttctttc atctccacga ccaattggaa ttctcgcatt caccaggtct
     1801 cgcttaacca tcaattacct gcttaccccg gttctgcgac caccagagtc gtttggcgca
     1861 tgtgggttcg gagctaacag aggatgtctt gtcatgtcca acacgtttta tgtcaccacc
     1921 ccaatctatt atgtcaacga tgtgccgcac attggccacg cctacaccac catcgcggcc
     1981 gatgttctag cccggtttta tcggctaaca ggagatgagg tttttttcct caccggacta
     2041 gacgaacatg ggcaaaaagt ccaacaggcg gcggccaagg ccgggatcga tccgcaggca
     2101 cattgtgacc tcctcacgcc aaagttcaag aatctctggt cccaattaca tatctccaac
     2161 aatgggttta ttcgcaccac cgatcttcaa catcaggcta tcgtacaacg ctaccttcaa
     2221 gccctctatg acaaaggctt gatttatcag gccgaataca ccggctggta ttgcactttt
     2281 gacgagcgat tttggacaga aaaggatgtg gagaatggcc tgtgcccgga ttgcctgcga
     2341 ccagtagagc aggtcagtga acgaaattat ttttatcgca tgagtcgctt tcagaaccgg
     2401 ttgaaggagc atatccaaac aaatcccaac ttcattcagc cggacacccg tcgcaatgag
     2461 gttctagggt ttctgcaaaa gccccttgag gatctgtcaa tttcccgacc caagtcacgg
     2521 ttatcgtggg gcatcgaatt accatttgat cgggagtgcg tggcctatgt atggttcgac
     2581 gccttagtga attacctgtc agccctggag tacgtcgctc caactcccag caccacaaaa
     2641 ttctggcccg cgtcgctcca tctggtcgga aaagatatcc tgaccaccca tgccgtgtac
     2701 tggtcgacca tgttaatggg cttagaactg ccgctccccc aatccatctt tgcgcatggc
     2761 tggtggacgg tgaatgggga aaagatgtca aaaagtcgag ggaatgttgt cgatccctat
     2821 gccgtggtcc aagaattcgg cgctgatgcc tttcgctatt ttctgttgag ggaagtacca
     2881 ttcggccagg atggagattt ctccatcacg gcattcggta cccggtataa cgctgatttg
     2941 gcaaacgacc ttggcaatct gctgtcccga actcttacga tgctggccaa tttttccaac
     3001 aataccgttc ctcaacccaa cgccaaacac gaaaccgatc aggatcgtca actccagcat
     3061 gcggcttcat ccctttttcc ctccattgac acacaccttc gccacttaga atttaatcgc
     3121 gcactggaaa ctatgtgggg tttggtccag tcagctaatc aatatattga taagaccgct
     3181 ccatggatgt tggccaaaga tcctgatcat acccctcgcc ttcacacggt cctatttcat
     3241 ctcacggagg tcttgcgatt cctcacggta gccgtacacc cgtttatgcc acagatcgcc
     3301 gaaacgatga agacccgact gggattatcg atggattttt ccacgcccgt gttgcaagaa
     3361 ttccctcgtt ggggagcata cctttttacg gggcaaacca gtaaaggcgc ttcgctattt
     3421 cccagaaaag attcccgtcc atcggactcc cagaagagtc ccgaacagaa aatgcctatt
     3481 cacccaaaca ggacacacat ggaaacacct cagcccgttc catccatcca accgcaatcc
     3541 ccacccgcag acgtgataca aatcggcatt gccgatttcc aaaaagttca attgaaggtg
     3601 gccaaagtct taacggcaga acgtgttcca aagtctgaaa aactgctcaa gctccaggtg
     3661 gatatcggaa cagaacagcg tcagatcgtg gctggaattg ggaaaaagta tgccccagaa
     3721 gaaatggtgg gccgaaccat tgtcgtggtg gcgaatctca agccagcaaa actgatgggg
     3781 attgaatccc aaggcatggt attagctgca ggagatcaag aggtccttga acttcttaac
     3841 atgtcccagg agatccctgc cgggacaaga atcaaatgat ggttttcacg cttctttcac
     3901 tcgaaaagtc atgacggaca tctgcccaaa atttttcttg agaaggcccg ctgacgtcct
     3961 tgcacggaat gaagaaaatg catggtcaga gaaatgtcag ggataatcac gaggagggaa
     4021 attcaacccc attggcaaag agtctcacat gggagaactg ccgcgctgcc acacgaaggg
     4081 ggaaaggcta atacgcctcc cgatggatca accccttcca aagggtcata atcataattt
     4141 ttacatccag ccataaggac caatgctcga tgtaatacat gtcgtaggct aaccgttttt
     4201 cccaagaggt attccctcgc aatccattaa tttgggccca acccgtaatt ccggctttca
     4261 tggtgtgacg caggttatat tggggaatct gggcccggaa tttctcgata aactccggtc
     4321 gctcgggacg cggccccaca atactcatct ctccctttaa aacattccag aattgtggca
     4381 attcatccaa actggtccgt cgcagaaacg tcccgatggg cgtcctgcgt gggtcatggg
     4441 ctttggccca gacgggtccc gtcttttctt cggcatccat acgcatagtc cgaattttga
     4501 caatattgaa cgatttccca tcgaggccaa cacgagtctg ccggtagaaa actggacctg
     4561 gagaggtgag cttgatgagt accgcaatca tcaatgcgat tggtgaggcc accaccaggg
     4621 ccacggcggc accacagata tcaaccatcc gcttcagaag caaattccac ccgtaaagag
     4681 gagatccttg cagagtaata ataggaaggc cctcaaacat ttcagcttct gcccgcaacg
     4741 tcacgaactc ataaatggat ggaatgatct tcactcctac tgtggtggca gacaggattt
     4801 tcatcagtcc ctcagcttca cattccacct ccggaggcaa acacaaaaat acgatgtcca
     4861 cctggctggt taaaatatct gccgcctggg caaacgttcc gataacgggt gtcccgtcca
     4921 atacttgacc aaccttttcg gcattcctgg tcaaataccc gcgaacctgg aggccaagtt
     4981 cgggatgcat tttgagcgta tcgccaaccc gctgcccaag ttttcctgcg ccaataataa
     5041 ccacctggcg ttggttgtat cccattcgcc ggaaaaccct gagaacttcc cgaaagacca
     5101 tacgggaaaa gcccaacacc accagattca atagccaaaa atacatgatc accaaacggg
     5161 aaaattcaaa cggcttcgag aaaaaggtca atgcgaccag gatcagaata ctcagcgtat
     5221 tggccttggc aatgtccaca aattccgcca gatgagagcc cattctacga gggcgataca
     5281 gattaaatga atagaaacaa attccccata cggcgacaat tggaaaaagg agccagagat
     5341 acgggtcaat aggagggatg cctttggtga tgggaaacag gggcactgaa aagcgaatga
     5401 agtaggcggc cacccaacac acgcatatca caaccaagtc ggaaaggaag agcagatttt
     5461 ttaagaattc actgtggcgt ttcaacatgg tggggtacga cggaattcgc gataacgaga
     5521 catgatcacc tgctgcatcc gttgcttaaa atgagaacga tcaaacggtt caacatgtgc
     5581 ccgaattgca tccggattga aatctgtgag gtgctgcgtg aacaactgaa tgctttcaat
     5641 tatggcttgg accgactgtt catagtgaaa gacccccgtt ggaacagaag aagatccact
     5701 tccgtgaaga ccacgttcta aatgattttc gcctggctta tgcttaggat tgagagggac
     5761 gattgtttca agggctccac cctttccata ggcaatgacc ggtttcccgg ccgccatggc
     5821 ctccaaaggc acaataccaa aatcctcttc accggggaag agaacggcca gacaccggct
     5881 gtaaaattct ctgacacgat ggtctggctg ccagccaaga aattcgatac ttggaccggc
     5941 cattcgcctt aagcgtttct catcttggcc ctgcccaata attttcaatg gaagccccag
     6001 ctcattggcc gcagcaatcg ccagatcgac ttttttgtag ggcgcaaagg ccgtgaccat
     6061 caagtaaaac ccttcatggc ggtgcgacac gtgaaaggtc tgccaatcaa caggagggta
     6121 gaccacgcaa gccgcccttc catactggcg tgcaattcgc tctgctacgt tatgcgaatt
     6181 ggcaataaaa caactcacat gggcattgga ttccatatcc cactgctgaa gtcgcgttcg
     6241 aaaaaggccc atgcccagtc tgccaaaatt ccaaatcccc ggcttaacaa aataggtatc
     6301 aaatccatcc cagatatacc gcatgggggt atgaacgtac gaaatatgac aggtctcgcg
     6361 aggcactcga atgcccttgg ccacacaatg gctcgaactg atcaccaaat catactgatg
     6421 gaattccaat cgctggatgg ctgaaggaaa gaaagggagc aaataccgat atcgactctt
     6481 tgcaaatggg actcgattaa gaaagctggg aataataaga tgccgctcaa tcgtctccga
     6541 cacactgccc ttcacatgaa acagcgtata aattggcgca tcaggtaata attcacacaa
     6601 cgcctcaagg caccgctcac cccctcgcat accagtcagc caatcatgaa ccagcgcaac
     6661 tttcacttgt gccctcaatg acctgcatgt gaggcagaca tttcctgata tattttcaga
     6721 atcgaagcca tcatggtttc gatggaaaat ttttcttgta tagtctcttg tgccttctta
     6781 atgagggaga tcctgagatc ctcattgttt gtgattattt tgaccctttc ggcaagatct
     6841 tctcctctcc ttttctctac cagcattccg tttacctcgt catgaatgat ttcttccaac
     6901 ccactcacat tcatgctcac aaccggaact cccatgccca tagattctaa gaccaaaacc
     6961 ggcaggcctt cccacctgga ggttaaaacg accacgctga acgtagcgag gatttccaga
     7021 gcctctccat gagacaactc ccccaccatg tgaacccgtc cacctaaacc ccgcctggct
     7081 atttcctgtt tgatcatggg agccaaagcc ccatcaccaa caatcgtagc cgaatactca
     7141 ggcaagaact ccatcatctc tacaaaaagg agaggatcct tctgaggttc taatcgtccg
     7201 ataaagccaa tatgcaggag cgcctggggc aaaggcggtg gaagattttt caaagagagc
     7261 cctggataga taactgacct tttagcgcta cctgatagca attgaaattt tttcgcaaga
     7321 accacatcat atttcgacac aaaaatagaa tgacacgcac gtcgcagatt ccaacgttcg
     7381 gccattaacg ccattcggcg aatgatcggt gacttttcaa caaaatgata tccatggacg
     7441 gtatagacca tcgggacttt caagagagat actgcaaaga aaaaccctgc acgcccacca
     7501 tggacatgaa ccagatcagg atgaaaggcc ataaccaacg ctcgaagttg gaaagggata
     7561 gcaggattga gccgactaat gaaaaaccga agcccctgac agggaatacc aagagctccg
     7621 gcttccttca ttaaatatga ttgttcttcg gtaatgagtt gaaaggaaac ggcatgccgt
     7681 aagcctttga aaatctgtag aacatgcgtg gttcctccac ccggctttcc atcagcaaca
     7741 atttggagga cccgcattac gtcccaaatt tcccaagcaa atttcctgtg attcttagac
     7801 cacacactat cgcagagcgc gacctattcg atcggttcca agaccacggt acatacctaa
     7861 tccagcatag atactttgca aagcccacaa ggcccatgcg accggacgac caaaatgttt
     7921 ccggaaaaaa taatattgat taacataata aatttctttt tgtttagaaa gatcg
//
LOCUS       DATIAL010000465         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1266480, whole genome shotgun sequence.
ACCESSION   DATIAL010000465 DATIAL010000000
VERSION     DATIAL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266480"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VLA60_14400"
     CDS             complement(<1..257)
                     /locus_tag="VLA60_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10598.1"
                     /translation="MTTLFPKLRLIAVFFLLTHIGMGSPSSQAGEAHDLLAQAFSSYE
                     HQQWTAAIAPLERALTLYPGYAEAHHLLGLILNNLDQPAKA"
     gene            complement(295..879)
                     /locus_tag="VLA60_14405"
     CDS             complement(295..879)
                     /locus_tag="VLA60_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSF10599.1"
                     /translation="MVRRHNLMIYILLLWCTLTSNPISLAGNPPESSEPIETLEVIEV
                     TGTTVTKASITYSFPLPSFNHSWTPTTFHHLAVPDLHFVNLPQPPMPRILLDQIGTTR
                     GRMTSVKPLKTERPLYPRMAREQGWQGKVVLRTQITADGIVKNATVQESSGFSLLDES
                     AVQSVKSWLFEPAKNGEFAVASTVDLPIRFDLLQ"
     gene            complement(1086..1379)
                     /locus_tag="VLA60_14410"
     CDS             complement(1086..1379)
                     /locus_tag="VLA60_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10600.1"
                     /translation="MTYRKSLTMVFILMLFLIMGSMHRTYASHITDHTHHHTPTTHST
                     GVCSWMCAAAQSISGDNLDISPSFQLLTIMGWPFPSVPSLVSTHFLTTRAPPR"
     gene            1665..>3099
                     /locus_tag="VLA60_14415"
     CDS             1665..>3099
                     /locus_tag="VLA60_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HSF10601.1"
                     /translation="MFVRLLNILFGSKNDRELKRLKPLVVRANEIEGSLQPLDDAGLA
                     AKTSIFKKRLEAGETLDDLLPEAFAVCREMSKRRLGMRHFDVQLLGGMVLHQGRIAEM
                     RTGEGKTLVATLPVYLNALEGKGVHLVTVNDYLAKRDAQWMGPLYHGLGLSVGIIQHD
                     ASFLFDPTYDAPDKRLQFLRPCSRREAYGADITYGTNNEFGFDYLRDNLVVTDLSQGV
                     QRELNFAIVDEVDSILIDEARTPLIISGPAEQSTDLYFQVDRIIPHLKREEDYTVEEK
                     TKTVSLTEEGNAHVEKLLGVDNLYDLKHLSLVHHVIKALQAHVLYHRDVEYVVKDGEV
                     MIVDEFTGRLMPGRRWSDGLHQAVEAKEGVKIANENQTLASVTFQNYFRMYNKLAGMT
                     GTADTEAAEFAKIYNLEVNVVPTNSTMIRIDNPDVVYRTEKEKFEAIADEIKEYHEKG
                     QPVLIGTISIEKSERLAALLKHRGVKHH"
BASE COUNT          798 a          673 c          844 g          784 t
ORIGIN      
        1 atggccttgg caggctgatc aagattattg aggattaatc ccaacagatg gtgcgcttca
       61 gcgtatcccg ggtacaaggt cagcgcgcgc tctaaggggg caatggccgc agtccattgt
      121 tggtgttcat aggacgaaaa ggcctgagcc agaaggtcat gggcttctcc agcctgactt
      181 gaggggctgc ccatgccgat atgggtgagg aggaaaaaaa ccgcgatgag tctgagtttt
      241 gggaaaagag ttgtcatggg ctaaccttat taatagtggc aatattccac aggtttattg
      301 aagcaaatca aatcgaatgg gtaaatccac ggtggacgca accgcgaatt ctccattttt
      361 tgcgggttca aacaaccagc tttttaccga ttgcacagca ctctcatcca ggagagaaaa
      421 tcccgaactt tcctgaacgg tggcattctt gactatgcca tcggcggtta tctgcgttcg
      481 tagcaccacc ttcccctgcc atccttgctc tctagccatt cgcggataga ggggacgttc
      541 ggttttcagg ggtttgacac ttgtcattcg cccgcgtgtt gttccgatct ggtccaggag
      601 aatacggggc ataggcggct gtggcaggtt gacgaaatgg agatcgggaa cagcaaggtg
      661 gtgaaaggtt gtgggtgtcc atgaatggtt gaaagagggc aacggaaatg aataggttat
      721 actggctttt gtcaccgttg tcccagtcac ttcaatgacc tccaatgttt caataggttc
      781 tgagctttca ggtgggtttc ctgccaagga aattgggttg gaggtcagcg tgcaccaaag
      841 caataaaata tagatcatca gattgtgccg acgtaccata agacctcttc cccttaagta
      901 acttgacgac cggcgttttt gccggtaaaa aaatcacttt cccccttaag aagttttccc
      961 ctcgttcggt ctctcctgac caggaagatg taggtagcca cctcctctga tcgcgctttg
     1021 taaaaccgtt ccccaactct actggttttg gtgaaaacgg tttaggagac aaacttaatc
     1081 taattttacc ggggtggagc tctggttgta agaaaatgag tggacacgag agaagggact
     1141 gacggaaacg gccatcccat gatggtaagg agttgaaaac tgggggaaat atccaggttg
     1201 tctccggaaa tggattgtgc ggcagcgcac atccaggaac agactccagt gctgtgggtg
     1261 gttggtgtgt ggtgatgggt atggtccgta atatgcgaag cgtaggtccg atgcatgctc
     1321 cccatgatga ggaaaagcat cagaataaaa accatggtga gactttttcg ataggtcatg
     1381 gtatcaacag accagaaacg actgaaaccc tatataataa cggaagctta tgctagggaa
     1441 ataccaaaga ttctgtcaag caaatggctg tccttcgcat cgtgtcgtcg tttgtcatat
     1501 gatgagccgt tgccgccata aatccgtttc tcgtgagttg attcccaccg gtaattcggt
     1561 atactaagaa aactgtacgg tttggaaagg tgtatctagt cctccatccc tatccatcgt
     1621 gagtttctcc aaccctattt ctgtatccga ggtgtaaggt cgatatgttt gttcgtctat
     1681 taaatatcct gtttggaagc aaaaatgacc gagaactgaa gcggctcaag ccactggtgg
     1741 ttcgagccaa tgaaattgaa ggttcgttgc aacctcttga tgacgcggga ttggcggcaa
     1801 agacttcaat ttttaaaaaa agactggaag ccggagagac cttagacgat ttactcccgg
     1861 aggcgtttgc cgtgtgccgg gaaatgtcca agcgccggct tggcatgcgc cactttgatg
     1921 tgcagttgtt agggggtatg gtcttgcatc agggccgcat tgctgaaatg cgcacgggtg
     1981 aaggaaaaac cctcgtcgcg acccttcccg tctacttgaa tgcgttggag ggaaaagggg
     2041 ttcatctggt tacagtcaac gattatctgg cgaagcggga tgcccagtgg atgggaccgc
     2101 tctaccatgg cttaggctta tcagtcggaa ttattcagca tgacgcctca tttttattcg
     2161 atccgaccta tgatgcaccg gataaacggt tgcaattcct tcggccatgt tctcgacgtg
     2221 aggcctacgg ggccgatatt acctacggga ccaataatga atttggcttt gattatctca
     2281 gggataatct cgtggtcacg gatttgagtc agggcgttca acgggagttg aactttgcca
     2341 tagttgacga agttgacagt attctcatcg atgaggctcg aacgccgctg attatttccg
     2401 ggcccgccga acaaagcaca gacctctatt tccaagtgga tcggataatt cctcacctca
     2461 aacgggaaga ggattatacg gttgaagaga aaacaaaaac ggtgtcgttg accgaggaag
     2521 gcaatgcgca tgttgagaaa ctcttgggtg tcgataatct ctatgactta aaacacctga
     2581 gtttggtgca tcacgttatt aaggctctcc aggcccatgt gctctatcac cgggatgtcg
     2641 agtatgtggt gaaggatggc gaagtgatga ttgtggatga gtttaccggt cgattgatgc
     2701 cgggacggcg ctggagtgat gggcttcatc aggctgttga ggccaaagaa ggggtaaaaa
     2761 ttgccaacga aaaccagacg ctggcatccg tgacgttcca gaattatttt cgtatgtaca
     2821 ataagctggc gggcatgacc gggacagcgg acaccgaagc cgcggaattc gcaaaaattt
     2881 acaaccttga agtgaatgtg gtgcccacca atagcaccat gattcgaatt gataatccgg
     2941 atgtggtcta ccggacggaa aaggaaaaat tcgaagccat tgccgacgag atcaaagagt
     3001 atcatgagaa gggccaaccg gttctgattg gcacgatttc cattgaaaaa tccgagcgtc
     3061 ttgcggcttt attgaagcat cggggtgtga agcatcatg
//
LOCUS       DATIAL010000466         2352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_728858, whole genome shotgun sequence.
ACCESSION   DATIAL010000466 DATIAL010000000
VERSION     DATIAL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728858"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VLA60_14420"
     CDS             <1..746
                     /locus_tag="VLA60_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HSF10602.1"
                     /translation="VLTTLHGRLDLPELKPIFAEFLDCPLISISDAQRKPLAWANWQQ
                     TIHHGLPEHLYTFHPDSQPYLVYVGRLTPEKRPDHAIEIAKRVGMPLKIAAKVDPVDR
                     DYFRTVIQPLLTDSLVEYVGEVTDSQKNDLIGNAYALLAPFDWPEPFGLVFIEALACG
                     TPILAYRRGSVPEIINNGDTGFICESVDEMVKSVPEISTLDRRHCRQMFEQRFTVERM
                     VQNYLSVYEHLLNRVDELPTSSLEEAVNF"
     gene            828..2291
                     /locus_tag="VLA60_14425"
     CDS             828..2291
                     /locus_tag="VLA60_14425"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF10603.1"
                     /translation="MSKWVDSLKSSGSWDLFQNPNFRQLWASQAVSQIGDGLTKVALL
                     WFVYQLTGSALKMTIIGLLQTIPPLLLSPIIGVYLDRLPKKPVMVTLDLIRAVLIALI
                     PILFLFDALSLEVLFVLVFFTAIFSTAFGPALAASLPLLVKPTELMSANSLLQTTTNL
                     GMLIGPAICGILIAWLGTENVLYVNAVTFLVSALCLLPIKGRLLTPAPGLSKAASNMS
                     QDLLVGMRFVFRERPVLRSLLFTTVFFTLGASAFIYVLPLFVNEYMKADPIWLGWLWS
                     SMGAGMLVTSFSLSCVKNLGRNARIILIGFSLVVGGSALQLLAHAHSIIWGFILVTII
                     GASTAAFTPIVWSIVQETTPNHLMGRVFTLINTASMAMASGGMVAFGGVADTFGALAG
                     ISMISFVFLGTGLAVIISNRSAIPGLRMFRWPSFEPSIRVDSQKAIGYLYPVHSMPES
                     IQRNWLWNVEWNPPTAHASSWRRLPQVVLQLISRAPP"
BASE COUNT          527 a          574 c          546 g          705 t
ORIGIN      
        1 ccgttttaac aacactgcat gggcgtctgg atcttccgga acttaaaccg atttttgccg
       61 aatttctgga ttgtcctctt atttccattt ctgatgctca acgaaaacca ttggcatggg
      121 ctaattggca acaaaccatc catcatggtt tgcccgaaca tctgtatacg ttccatcctg
      181 attctcaacc ctatctggta tatgttggac gactcacccc tgaaaaacga cccgaccatg
      241 ctatagaaat tgcaaaacgt gtcgggatgc ccttgaaaat tgctgctaaa gttgatcctg
      301 tggatcgcga ctattttcgc accgtcattc aaccgctctt aacggattca ttggtggaat
      361 atgtgggtga agttacggat tcacaaaaaa atgatttaat cgggaatgcc tatgcgctgc
      421 ttgcgccctt cgactggccc gagcctttcg gtttggtgtt tatagaggca ttggcttgtg
      481 gcacaccaat tttggcctat cgacgtggtt ccgttcctga aatcatcaat aatggggata
      541 cggggtttat ttgtgaatcc gtggacgaaa tggtcaagtc cgtccctgaa atatccactc
      601 tcgaccggcg ccattgccgg caaatgtttg aacaacgctt taccgtggag cgaatggttc
      661 agaattatct ttccgtctat gaacatctgt tgaaccgtgt ggatgagctc ccgacgagct
      721 cattagaaga agcggtgaac ttttaagagt gggtcttttc ccccaggcac atcatcatat
      781 tgacaaacat tatattgaca aacattcttc cgggattagg gaaacatatg tctaaatggg
      841 ttgattcttt aaaatcttcg ggaagttggg atttatttca aaatcccaac ttcaggcaat
      901 tgtgggcttc acaggcggtc tcccaaattg gagatggatt aaccaaagtg gcccttttgt
      961 ggtttgtcta ccaattgacg ggttccgcgt taaaaatgac gattatcggt ctattgcaaa
     1021 cgattccccc cctcttgttg agccccatca tcggggtgta cctggatcga ttgcctaaaa
     1081 aacccgttat ggtgactctt gatctcatcc gggctgtctt gattgctctc atcccgatcc
     1141 tatttctttt tgatgccctc tctttggaag tgctgtttgt tttagtattt ttcacggcca
     1201 ttttctcgac ggcctttgga ccggctctgg cggcctctct gcccctcctg gttaagccta
     1261 cggagttgat gtccgccaat tcacttctgc aaacgacgac caatctgggc atgttgattg
     1321 ggccggcaat ttgtggaatt ttaattgcct ggcttggcac agaaaacgtc ctctatgtga
     1381 atgcggtcac atttctggta tcggcattgt gtttattgcc gatcaaaggc cgtctgctga
     1441 cgcctgctcc cggactctcg aaagccgcgt cgaacatgag ccaggacctt cttgtaggaa
     1501 tgagatttgt gtttcgagag agacctgtgt tgcgttctct cctgtttacc actgtcttct
     1561 ttacattggg agccagcgcc ttcatttacg tgcttccctt gttcgtcaac gaatatatga
     1621 aagccgatcc tatctggtta ggatggctat ggtcttcaat gggagcaggg atgctcgtta
     1681 cctctttttc tttgagttgc gtgaaaaatc tgggaagaaa tgctcgaata atcctcatcg
     1741 gtttctcctt ggtcgtggga ggtagtgccc tccaactcct cgctcatgcc cattcaatca
     1801 tatggggatt cattcttgtc acgattatag gtgccagcac agccgcattt acacccattg
     1861 tatggtctat tgttcaagaa accacgccca atcatttaat ggggcgggta tttaccttga
     1921 tcaatacggc tagcatggcc atggccagtg gaggtatggt ggcattcgga ggagtagcgg
     1981 atactttcgg ggctttggct ggcatatcca tgattagctt cgtgttttta ggcacgggat
     2041 tggcggtaat tatttccaat cgttccgcca ttccaggact acgaatgttt cggtggccct
     2101 cctttgagcc ttctatccgt gtcgattctc aaaaggcaat tggttatctc tatcccgtcc
     2161 attccatgcc ggaatccatc cagaggaatt ggctatggaa cgtcgaatgg aatccaccaa
     2221 ctgcccatgc ctcttcatgg agaagactcc cacaggtagt tcttcaactt atttcccgcg
     2281 ctccgcctta gcctcagcac gatgcccgat cccttccgga aggaacccaa tgagtcaaga
     2341 gaggtatcta tc
//
LOCUS       DATIAL010000467         5533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1158888, whole genome shotgun sequence.
ACCESSION   DATIAL010000467 DATIAL010000000
VERSION     DATIAL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158888"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VLA60_14430"
     CDS             complement(<1..698)
                     /locus_tag="VLA60_14430"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HSF10604.1"
                     /translation="MNQTRTLTEEFRNLSELAQNVWWSWSPEGRAVFSYIDPTLWRLT
                     YHDPIKQLQRIASDRLEALGQDVVFLSLYREAMKAFHAYMEAKDHWFGRTYPQWQDRT
                     IAYFSAEFGLHRSVPLYSGGLGILAGDHLKEASDLGVPLVAVGFMYSQAYFRQVVDTN
                     GWQEAVYDSVDPMMMPIELARTPAGDLAKVQVQLGSRMVACVVWQIQVGRVSLYLLDT
                     DTPENSPEDRHLTAR"
     gene            complement(817..1713)
                     /locus_tag="VLA60_14435"
     CDS             complement(817..1713)
                     /locus_tag="VLA60_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10605.1"
                     /translation="MAKQSAENYESVLKDLAHTMMSVARESVGLIKRSDQKLAMKLQR
                     KQEWEIYLEFLRMLFNLVDRLSGFYIPIQGQKEFMNSLEDSVAHQLKTVLAPSLSSSE
                     VDDMEVTLSVGQVVADSRKTYEKFKFVVTDQTKERDQYFQFFSERIASKAGAPGKQEI
                     TAAAFLCGSAVVPALKSLFEDATKPKAQPATSAAHSVAEGAGSSGAAEKGSSSTQSGA
                     PEAAGKNLIKLISVVSRAQGEEVESWWGLHPQFRRDLPPDEAKELAKHMNRATRIVGE
                     RFAVVASLAQSGTNPNQPVGNA"
     gene            2074..4086
                     /locus_tag="VLA60_14440"
     CDS             2074..4086
                     /locus_tag="VLA60_14440"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HSF10606.1"
                     /translation="MDSTSPPSKVYVLKRPLEESTPPKLSRNYAEELNPQQLAAVEAV
                     QGPALVIAGAGSGKTRTLVYRVARLIDLGIVPSSILLLTFTRKASQEMLSRVGLLIGL
                     RAQQVGGGTFHSMANILLRRYGRPVGLEPGFTILDRGDSEDLLSLLRGQLGLNDIGKR
                     FPRKHTLAEIFGKTANTLESLEDIVINEYSHFGEYLGELQKLQAAYDAAKRQRQLVDY
                     DDLLVKLLELLVIDQQARETISQIFRYILVDEYQDTNRLQASLVRNLAATHDNVMVVG
                     DDSQSIYAFRGATFRNIMEFPDLFPGATIYKLEENYRSTQPILQLANKLIEAAPEKYS
                     KTLFTRKGEGPLPTVVEAMGENAQSRFIAQKILELREEGIPLHEIAVLFRSSFHAFDL
                     ELELTRRNLPFIKRGGFKFIETAHVKDLMAHLRIIHNPLDTVSWNRLLLLLEGVGPKK
                     AKDLIASILQAHGQYEILKHSTGRSASGLRHLAATLDQLTENPLTPAVLLGEVMEYYV
                     PLLKDQYDDYPKRIRDLEHLSVMAERYESLNDFLADLTLEPPNESVVDVEVPDRDDER
                     LILSTIHSAKGLEWQCVFVIWLVDGRFPSAYSFMGPEELEEERRLLYVAVTRAKHHLF
                     LTYPINVYDKMTGSVLTKPSRYLDHIPPSYFDTWSLVEEDQTYSWR"
     gene            4098..5204
                     /locus_tag="VLA60_14445"
     CDS             4098..5204
                     /locus_tag="VLA60_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10607.1"
                     /translation="MSKSLKNSKMLNCIRYFTLSYQRWQSVQKGSSIKAAGILTSGAY
                     GGVREHDKRPRTKLANFCNTLLVTYFGRMLGLAILVSLISVNMPSQAWSAEDTTLSRP
                     NPRHPWPALLQQADTLGLPTGFLKHIDPKFVTVHFEDLRTYAAEYHPHDHTMILNLRL
                     SFNEAGGALADLARMTHHDVALLYHELLHAYLDYLYATDHGQALTAEDQQVLEAANNQ
                     MACHYRFVRINPIRQLKGATELRFLSDQDTWEVMNETWAVFVGWVLWTKLELFQDHLT
                     TKSWTPALIEQWTQRLTDAVNSGELLGYYEPDDPEERQIARKRFIAPSNGLTPQDVEL
                     LLTTVLREPPELVQASTAVFEQYQTTMEPAPSCE"
     gene            5354..5429
                     /locus_tag="VLA60_14450"
     tRNA            5354..5429
                     /locus_tag="VLA60_14450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5387..5389,aa:Arg,seq:ccg)
BASE COUNT         1386 a         1627 c         1302 g         1218 t
ORIGIN      
        1 cgggcagtca gatgacgatc ctctggtgaa ttttccggtg tatcggtatc caaaagatac
       61 agcgaaaccc ggcccacttg gatctgccag accacacacg cgaccattcg ggaacccaat
      121 tggacttgaa cctttgccaa atccccggcc ggtgtccgcg ccaactcaat cggcatcatc
      181 atcggatcga cggaatcata cacggcttcc tgccaaccgt tggtatccac aacctgccgg
      241 aaataggcct ggctatacat aaatccgaca gccaccagcg gaacccccag atcactggct
      301 tctttaagat gatcaccagc cagaatccct aatcccccgc tataaagtgg aacggaacga
      361 tgcaacccaa attctgccga aaaataggca attgtgcgat cctgccactg aggatacgtt
      421 cggccaaacc agtggtcctt ggcctccata taggcatgaa acgccttcat cgcctcgcga
      481 tacagggaaa gaaacaccac atcttgcccg agagcctcca accgatcaga ggcaatccgc
      541 tgcaactgct tgatgggatc gtgatacgtc agtcgccaga gcgtgggatc aatatacgaa
      601 aagacggccc ggccttcagg agaccaactc caccacacat tttgagctaa ctcagaaaga
      661 ttacgaaatt cttccgtcaa cgtacgagtt tgattcatag gatatactca caaaaagatg
      721 gcatcatcca atggtgaaga gtgaaggatc ttcaccaacc ctggacacat gttgaagtgg
      781 tcaaagagaa cacttagaag agtcagttag agagggttag gcattcccaa caggttgatt
      841 tggattcgtc cccgattgcg ccaacgaagc cacaaccgca aaccgctcgc ccacgatccg
      901 ggtcgcccgg ttcatatgct ttgccaattc cttggcttca tcagggggaa ggtctcggcg
      961 aaattgagga tgaagccccc accaggattc aacttcctcc ccctgtgcgc gagacaccac
     1021 actgatcaac ttgatcagat ttttccccgc tgcctccggg gcaccggatt gtgtcgaaga
     1081 gctgcctttt tcagcagccc cagacgaacc ggccccttcc gctacggagt gagccgcaga
     1141 tgtcgccggt tgggctttgg gtttagtcgc atcttcaaag aggcttttga gagccgggac
     1201 gaccgcactt ccacacaaaa atgccgcagc ggtaatttcc tgcttgcccg gggctccggc
     1261 tttagaggca atgcgttccg aaaaaaactg gaaatattga tcccgctctt tggtttggtc
     1321 tgtgaccaca aacttaaatt tttcataggt ttttcgacta tccgccacca cctggccaac
     1381 ggacagcgtc acttccatgt catcgacttc acttgaactc aatgaggggg ccaggaccgt
     1441 ttttaattga tgggccacgg aatcttccaa actattcatg aattcctttt ggccctgaat
     1501 cgggatataa aatccagaca ggcgatccac caggttaaac agcatacgca ggaattccaa
     1561 ataaatttcc cattcctgct tccgctgcag cttcatggct aacttttgat cagaccgttt
     1621 gatcaagccg accgattccc gagccacgct catcatggta tgagcaagat ccttcagcac
     1681 actctcgtaa ttttctgcag attgtttggc cattttcaac tccgtaattg gctaaagtat
     1741 aaccgagggc ccgcgacctg tccaagccta atatggaagt gttaagccgc cagtggcgtt
     1801 ctcgccactt ggccgtgctc acctacttct tgtacgctcc gctcgtccaa gtagctgcgc
     1861 ccttactgga cgagcctttt tgaacactcc cccattgttt tcatttgctt ttggccactc
     1921 atctgctcgg gaaaagggga acatattcca aaattcaaga gaccgaatat cgcccgcgtt
     1981 gcaacccacc accacgtatg ctatacacgg ccaaaggtct tcgaaagacg aaactcgggt
     2041 gcatatctcc cgtcaattcc tgcccatgga ttcatggatt ccacgtcgcc accatccaaa
     2101 gtctatgtcc tgaaacgccc cctggaggag tccactccac caaaattatc gcgtaattac
     2161 gcggaagaat tgaatcccca acaactcgcc gccgtggaag ccgtgcaggg cccagccctt
     2221 gtcatcgccg gggccggcag cgggaaaaca cgcacgctcg tgtaccgggt cgccaggctc
     2281 atcgacctgg gcattgtgcc cagctccatt ttactcctga cctttacccg aaaagcctca
     2341 caggaaatgc tcagccgggt cggactcctc atcggactcc gcgcccagca ggtcggcggt
     2401 ggaacctttc actcgatggc caatatcctc ctgcggcgtt atggccgccc cgtcggactg
     2461 gaaccagggt ttacgatact cgatcgggga gattccgaag atcttttgag cctcctccgt
     2521 ggtcaattgg ggcttaacga catcggcaaa cggttccccc gcaaacatac cctggccgaa
     2581 atattcggga aaacagccaa cacactggaa agcctggaag acattgtcat caacgaatac
     2641 agccatttcg gggagtatct aggagaactc cagaaactcc aggccgccta tgacgcagcc
     2701 aagcgccaac ggcaactggt cgattacgac gatctcttag tcaaactcct tgaactcctg
     2761 gtcatcgacc aacaggcccg ggaaaccatt agtcagatct ttcgctatat tctggtggat
     2821 gaatatcaag acaccaatcg cctgcaagcc tcattggtcc gcaatctggc ggccacccat
     2881 gacaatgtca tggtggtggg cgacgattcc caatccatct atgcctttcg tggtgccacc
     2941 ttccgcaata tcatggaatt tcccgacctc ttcccgggtg cgaccattta caaattggag
     3001 gaaaactacc ggagcacgca accgattctc caactggcga acaagctcat cgaagcggca
     3061 cccgaaaaat acagcaaaac cctcttcaca cgaaaaggag agggaccatt acccaccgtc
     3121 gtggaggcga tgggagagaa tgcccaatcg cgattcatcg ctcaaaaaat tctggagtta
     3181 cgggaagaag gtatcccctt acatgagatc gccgtgctgt tccgatccag ctttcatgcc
     3241 tttgacctcg aattggaact cacccggcga aatctcccct tcattaaacg aggcgggttc
     3301 aaattcatcg aaaccgccca tgtcaaagac ctcatggccc acttgcggat cattcacaat
     3361 cctttggaca ccgtcagttg gaaccggctc ctcttgctcc tcgaaggggt cggccccaaa
     3421 aaagccaaag atctgatcgc atccattctc caggcccatg gccagtatga gatcttgaaa
     3481 cacagcaccg gccgctccgc ctccggattg cgtcacctgg ccgccacact ggaccagtta
     3541 acggaaaatc ccctcacccc ggccgtactg ctcggggagg tgatggaata ctatgtccct
     3601 ctcctcaaag atcagtatga cgattacccc aaacggattc gagacctgga acatttatcc
     3661 gtcatggcgg aacggtacga aagtctcaac gacttcctgg ccgatctcac gcttgagccc
     3721 ccgaatgaaa gtgtggtgga cgtcgaagtc cccgatcggg acgacgagcg gctgattctt
     3781 tccaccattc actcggccaa gggattagaa tggcaatgcg tctttgtcat ctggctggtg
     3841 gacggacggt ttccctcagc ctactccttc atgggccccg aagaacttga agaagagcgc
     3901 cgactgctct acgtggcagt caccagagcc aaacatcacc tcttcctcac ctaccccatc
     3961 aatgtgtacg ataaaatgac cgggtcggtc ctcacgaaac cctcacggta tctggatcac
     4021 atcccgccat cctatttcga cacctggagt ttagttgaag aagatcaaac ctattcctgg
     4081 aggtaatttt tagtagtatg tcgaaaagcc taaagaactc taaaatgctc aactgtattc
     4141 ggtacttcac attatcctat cagcggtggc agagtgttca aaaaggctcg tccatcaagg
     4201 ctgcaggcat tttgacgagc ggagcctacg ggggagtacg tgagcacgac aaaaggccga
     4261 gaacgaagtt ggcgaacttt tgcaacactc tgctagtgac atatttcggt cggatgttag
     4321 gcctggccat tctggtcagt ctgataagtg tgaacatgcc aagccaagcc tggtccgctg
     4381 aagacaccac cttaagtcgt cccaaccccc ggcatccgtg gcctgccctc ctgcagcaag
     4441 ccgataccct ggggctgcca acggggttcc tcaaacacat cgaccccaaa tttgtcactg
     4501 tacactttga agacctccgc acctatgccg cagaatatca tccacacgat cacaccatga
     4561 ttctcaacct gcgtctgtcc tttaatgaag ccgggggagc gttggccgat ctggcccgga
     4621 tgactcacca tgatgtcgcc ctgctctatc atgaactcct gcatgcctat ctggactacc
     4681 tctacgccac cgaccatgga caagcgctga ccgcggaaga ccagcaggta ttggaggccg
     4741 ccaacaacca gatggcctgt cattatcgct ttgtccggat caatcctatt cgccagctca
     4801 aaggcgccac cgaactccga tttctctcgg atcaggatac gtgggaagtc atgaatgaaa
     4861 cctgggcggt ctttgtcggc tgggtgctat ggaccaaatt ggaactcttc caggatcacc
     4921 tgaccacaaa aagctggaca ccggcactca tcgagcaatg gacccaacgg ttaacggatg
     4981 cggtgaactc cggtgaatta ttgggctact acgaacccga tgatccggag gaacgccaga
     5041 tcgcccgtaa acgcttcatt gccccttcca atggcctcac tccgcaagat gtcgaactat
     5101 tattaaccac cgtcctaagg gaaccacccg aactggtcca agcttccacc gcggtgtttg
     5161 aacagtacca aaccaccatg gaaccggctc cctcttgcga ataaattgag ggcctccggc
     5221 aggtagacat cacctaaaca tccatgctag cttcagaact tttccggggc ggttgccctc
     5281 tgtttttcct ctcgcatggc ggagaggcca cgagaactag aggccggatg taattgcaca
     5341 tgaacacaat cttgcgcctg tagctcagtg gatagagcac ttgcctccgg agcaaggggc
     5401 cacaggttca aatcctgtca ggcgcaccat gcacatgaat atttcgaaag ttcccctccg
     5461 tttgtaagga ggggcgaggg gaggtaggat tcagaattta ttctccttat aggtggacga
     5521 aagacccgtt gga
//
LOCUS       DATIAL010000468         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1374421, whole genome shotgun sequence.
ACCESSION   DATIAL010000468 DATIAL010000000
VERSION     DATIAL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374421"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /gene="rsmH"
                     /locus_tag="VLA60_14455"
     CDS             <1..539
                     /gene="rsmH"
                     /locus_tag="VLA60_14455"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSF10608.1"
                     /translation="RMDQTQRVTAADLVNFLPEHELADVIFAYGEERYSRRIAHAIVQ
                     ARKTGLIQTTQALVAILELALPRVYKKGRLHFATRTFQALRIRVNRELEVLEPALGDA
                     IDLLKEGGRVCVVAFHSLEDRIVKHAFRAMARGPHPRIALLTKKPVIPSELEIRHNPR
                     ARSAKLRVAERLPEGEAL"
     gene            536..883
                     /locus_tag="VLA60_14460"
     CDS             536..883
                     /locus_tag="VLA60_14460"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HSF10609.1"
                     /translation="MKKNVFLGVMIMLGVWCLVYVGVKIDMWRLGYAIEDLETQRAAL
                     KKQQEGLQVSLSELTDPQRVAQRAHNQLGLIVPQEGQIVMVSLESLPHPEKDGDLPVR
                     LVREFSHSLLSIP"
     gene            931..2649
                     /locus_tag="VLA60_14465"
     CDS             931..2649
                     /locus_tag="VLA60_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSF10610.1"
                     /translation="MVCLSLLLGFFIIGGRLFYLQVLQAEVGAHQAQQQHQKTVVLQP
                     DRGVIVDRQGHPLALNVEVASLYVRPLSLQDPQRTARSLAPVLQMPVAQLRDLFTRNQ
                     PFVWVKRHVPENIVKKLEAMKISGMGLIREPHRFYPKGELVSHIVGFAGIDSQGLEGV
                     ELQYESYLKGERNLVRYQRDALGRKISSPQNQDVVTLPTGYHLTLTVDEVLQFIAEEE
                     LAQALKRSGAKSGSIVMMDPLTGAILAWALHPTFDPNHVAQFSAQDWRNRAVTDPYEP
                     GSTLKIVVAAAALEEQVMSPETLIYGGDGQMAVAGTIVHDPAKTSWMTFSEAVAQSSN
                     VGAIKVALELGPDRVFEYLKAFGFGEKTDIDLPGESSGILREPHKWSGRSLSSIAMGQ
                     EIAVTPIQMVVAMSVVANGGWLMTPYVVSSVLDSQGHVILTKDPQPKRRPISMETAGA
                     LTRILETAIETGTGRRAKLAEYRAAGKTGTAQKFDPEIGSYSSSKIIASFVGFAPVEQ
                     PRLTMLVMIDEPKIGNWGGEIAAPVFRKVAERVLPHLGVLPGKSPIIRTAAATSPISL
                     SQPLVQ"
     gene            2689..>3154
                     /locus_tag="VLA60_14470"
     CDS             2689..>3154
                     /locus_tag="VLA60_14470"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HSF10611.1"
                     /translation="MKIEGNLQCEVVSITEDSRQVKPGSVFVAIKGEHQDGHQFVRQA
                     QIQGAVGVVVEEDVVGGLESSQGGKSSAVIRVKNSRKTLGLLAARVHGIPSAQLKMVG
                     VTGTNGKTTVTHLAKSLLEVQGHQVGLLGTVGYVFGKELRVASHTTPAPVELQ"
BASE COUNT          798 a          712 c          861 g          783 t
ORIGIN      
        1 tgcgaatgga tcaaactcaa agagtcacgg cagctgattt agtaaatttt ctcccagaac
       61 atgaattagc agatgttatt tttgcttatg gagaggagcg ctattcccgt cgaattgctc
      121 atgccattgt tcaggccaga aagaccggtc ttattcaaac gactcaagca ttggtggcca
      181 tactcgaatt agctcttcct cgcgtctata aaaagggacg attgcatttt gccaccagaa
      241 cgtttcaagc gcttcgcatc cgtgttaatc gggaattgga agtgttggag ccagccctgg
      301 gagatgccat tgaccttttg aaggaaggtg gacgagtctg cgtggtggca tttcactcgt
      361 tagaagatcg gattgtgaag cacgcattcc gtgcaatggc acggggaccg catccgagaa
      421 ttgccttgtt gaccaaaaag cccgtcattc cgagtgaatt ggaaattcga cataaccctc
      481 gtgcccgaag tgcaaagtta cgtgtggctg aacgcctccc agaaggagaa gctttatgaa
      541 aaaaaacgtg ttccttggcg tcatgattat gttgggggtg tggtgtctgg tatacgtagg
      601 agtcaaaatc gatatgtggc gcttggggta tgctattgaa gatttagaaa ctcaacgagc
      661 agcattgaaa aaacagcaag aaggattaca ggtgagcctg tcagagttaa cagacccaca
      721 acgagttgcc caacgggcgc ataatcaatt aggactcatc gtgcctcaag aaggccagat
      781 tgttatggtg tcccttgaat cgttgcccca tcctgaaaaa gatggggatc ttcctgttcg
      841 cttggtcagg gaattttcac atagcctatt gagcattccg tgagaacgcc atctcaggac
      901 attcatcctg tctgcagaat gcgaagtggc atggtctgtt taagcctctt attagggttc
      961 ttcattattg gtggccgtct gttttacctg caggttcttc aggctgaggt tggggctcat
     1021 caggcacaac aacaacacca gaagacggtg gtgctgcagc ctgaccgggg tgtgattgtc
     1081 gacagacaag gtcatccact ggccctcaac gtggaggtgg cgtcactata cgtcaggccg
     1141 ctctcactcc aggatccaca acgcaccgcc cggtccttag cacctgtttt gcaaatgccg
     1201 gtcgctcagt tgcgtgacct ttttacacgg aaccaaccct tcgtgtgggt gaagcgtcat
     1261 gttccggaaa atatcgtcaa aaaattggag gcgatgaaaa tctctggcat gggattgatc
     1321 cgcgagcctc atcgttttta ccccaagggg gaactggtat ctcatatcgt ggggtttgcc
     1381 gggatagata gtcagggttt ggaaggggtg gaacttcagt atgaatccta tttaaaagga
     1441 gaaagaaacc tggtgcgata tcaacgtgac gccttgggaa gaaaaatctc ttctccacaa
     1501 aaccaggacg tggttaccct accgaccggt taccatttga cgttaaccgt ggatgaggta
     1561 cttcaattca ttgcggaaga ggaattagcc caggcattaa agcgaagcgg cgccaaatcg
     1621 ggaagtattg tgatgatgga tcccttaacc ggggcaatcc tggcgtgggc gcttcatcct
     1681 acctttgatc ccaaccacgt ggcccaattt tctgcgcaag attggcgaaa tcgagcggtg
     1741 acagatcctt atgaaccggg atcaacctta aaaattgtgg tggcggcggc tgcactggaa
     1801 gagcaggtca tgtccccgga aacgttgatc tatggtggtg acgggcaaat ggctgtcgcc
     1861 gggaccattg ttcatgatcc ggccaaaacc agttggatga ccttttccga agcagtggct
     1921 cagtcaagca atgtgggtgc cattaaggtc gcattggaat tagggccgga tcgcgtcttt
     1981 gaatatttga aggcgtttgg atttggagaa aaaaccgaca tagatctccc gggagagtct
     2041 tcagggattt tacgagaacc ccacaaatgg agtggtcgaa gcttgtcttc gattgccatg
     2101 ggacaagaaa tcgcggtcac tcccattcaa atggttgtgg ctatgtccgt ggtggctaat
     2161 ggcggatggc ttatgactcc ttatgtggtt tcctcggtat tggattcaca agggcacgtc
     2221 attctgacaa aagatccgca gcccaagcgt cgccccatct cgatggaaac agctggtgca
     2281 ttaacccgca ttctcgaaac ggccatcgag acaggcacgg gcaggcgtgc caaacttgcg
     2341 gaatatcgag ctgcgggaaa gacggggaca gctcaaaaat ttgatcccga aatcggatcg
     2401 tactcttcct caaaaatcat tgcttcattc gtaggatttg caccggtcga acagcctcgt
     2461 ctcaccatgt tagtgatgat tgatgaaccc aaaattggta attggggggg cgaaatcgct
     2521 gcgccagtct ttcgaaaggt ggcagaacga gtcctgccgc atttgggggt cttgccggga
     2581 aaatccccaa ttattcgaac ggctgctgct acatcgccca tttccttatc gcagcctcta
     2641 gtacaataac ctgtgacgct tcaagagctt atttcttctt tagatagcat gaaaattgaa
     2701 gggaatcttc aatgtgaagt cgtgtctatc acggaggatt cccggcaggt gaagcctggt
     2761 tcagtgtttg tggcaatcaa gggagaacac caggatggtc atcaatttgt gaggcaggcg
     2821 caaatacaag gggctgttgg tgttgtggtg gaggaagatg tcgttggtgg acttgaatca
     2881 tcccagggag gaaaatcctc cgccgttatt cgtgtgaaaa attctcgaaa aactttggga
     2941 ttgttggccg ctcgagtcca tggcataccg tccgctcaac tgaaaatggt aggagtgacg
     3001 gggacgaatg ggaagacgac ggtgactcat ttagccaaat ccttactgga ggtccaagga
     3061 catcaggttg gattgctggg aacggtggga tatgttttcg ggaaagaact ccgtgtggct
     3121 tcacatacaa cacctgcccc cgtcgaacta cagg
//
LOCUS       DATIAL010000469         3128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1590706, whole genome shotgun sequence.
ACCESSION   DATIAL010000469 DATIAL010000000
VERSION     DATIAL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590706"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VLA60_14475"
     CDS             complement(<1..439)
                     /locus_tag="VLA60_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10612.1"
                     /translation="MSPQEWLNVLKSAYLQGFIRRGGSAVKFAVVEEAQNAKAVSVGV
                     GALSREEGFVTIHADSHCTKVQLIDLLFKEMARQIDWDALAFEYVKKLFREHQWKIPE
                     RREECCWSSLASLNSCDEATMHREVNAWLEGSLFHDFQMSRDFR"
     gene            complement(520..1407)
                     /locus_tag="VLA60_14480"
     CDS             complement(520..1407)
                     /locus_tag="VLA60_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10613.1"
                     /translation="MSAEGIDNTLEACGSSSATGVLELPDAPPKAVKKNVNLEQALAD
                     IEEQAGGLVKTTKQVMASFKKIKDAAKVGDLVNLRKLLEEGKEVTLSLGCDFVKTQER
                     LNFDEAGYLEGKAFRQELLTTAQQMGVNLYEHDGYLFSYPVLLRILHKERAVAIDRMR
                     ENRLRPSVLVKRLKEVQNKPLRFKPQTFLEMVFTAYSIVVAGRGKHLIGKGTVIPLLE
                     LYQLLTLLPWQANEYTRQEFARDVYLLDKSGTISTKNNHRANFHASTGVRDANKTLTV
                     IAQGGREKTYYGISFVQTG"
     gene            1626..2447
                     /locus_tag="VLA60_14485"
     CDS             1626..2447
                     /locus_tag="VLA60_14485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF10614.1"
                     /translation="MVERFLQTLFMENMAPFAVITGASRGIGAEYARALAAQGYHLLL
                     VARDHNRLQDLSKEIQQTTSMQVWTETLDLAKPNAAGTLYQLAQSYRPHVSLLVNNAG
                     FGLYGPFTGMPLSTIQDMLQVHIHATTESTRLFLPGMMNRREGAIINVASVAGFFPIP
                     YMAEYAATKAFIISFSEAVAMEAKENEVTIQVCCPGYTETDFHRTAGHCPRHISSPHA
                     PYDVVRKSLKALKSRQTLVTIGWQGLATEWITPFIPKKWLRRLASRFVRPNSPSH"
     gene            2480..2911
                     /locus_tag="VLA60_14490"
     CDS             2480..2911
                     /locus_tag="VLA60_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HSF10615.1"
                     /translation="MAHSYFHSIIVPLSICSVCILAFGCSGPRPILYPNNYLQQVGPD
                     RAEQDIEECQQLAEDYVPEHDASTVAGNTAVGAGAGGAVGAVSGAIRGGVGIGAAIGA
                     ATGATVGFIRGLFQASQPTPAHKAFVSRCLSERGYESIGWE"
BASE COUNT          788 a          837 c          695 g          808 t
ORIGIN      
        1 atcgaaagtc ccgactcatc tggaaatcat gaaacaagct cccttccaac catgcattca
       61 cttctcggtg catcgtggct tcatcgcaac tattgagaga ggccagagaa gaccagcaac
      121 attcctcccg ccgttcagga atcttccatt gatgctctcg aaagagtttt ttgacatact
      181 cgaacgccaa ggcatcccaa tcaatttgcc tggccatttc cttaaacaag agatcaatca
      241 attgaacctt cgtgcaatga gaatccgcgt gaatcgtgac aaagccttcc tcccgtgaca
      301 gcgcccctac ccccacactc accgctttgg cgttctgagc ctcctccacg accgcaaatt
      361 tgacggccga tcctccacga cgaataaacc cctgaagata ggcagatttg agaacgttca
      421 accattcctg tggagacata aattttccct caatatcctg ttaatctcat tcgtcttctc
      481 ggttcaccct agggaaactt aaatacggtc ctgacccgtt tatcccgtct ggacaaagga
      541 aattccataa taggttttct cccgtccccc ttgcgcaatc acggttaacg ttttgttggc
      601 atcccgcacg ccggtactgg cgtgaaagtt tgcccgatga ttgttttttg tggaaatggt
      661 tccactttta tctaataaat aaacatcccg ggcaaattct tgtctggtgt attcattggc
      721 ctgccacgga aggagcgtca agagttgata cagttccaag agtggaatca ccgttccttt
      781 gccaatgagg tgcttccctc gacccgccac cacaatggaa taggccgtaa agaccatctc
      841 caaaaacgtt tgcggcttaa atcgaagggg tttattttga acttccttta atcgtttgac
      901 caaaaccgaa ggacgaagcc ggttttcccg cattcgatca atggcaaccg cacgttcttt
      961 atgaagaatg cgtaataaga ccggatagga aaataaatag ccatcgtgct catagagatt
     1021 gacacccatc tgctgggcag tggtgagaag ttcctggcga aacgctttgc cctccaaata
     1081 tccagcttca tcaaaattca gacgttcctg ggttttcaca aagtcacatc ccaggctcag
     1141 cgtcacttcc ttgccttcct ccagcaattt tcgcagattg accaaatcac ccactttcgc
     1201 tgcatcttta atttttttaa atgacgccat cacctgcttt gtggttttca ctaatccccc
     1261 ggcttgctct tcaatatcag ccaaggcctg ctccaaattg acgttcttct tgacagcctt
     1321 tggaggggca tccggcaact caagcacacc agttgccgaa ctcgatccgc aggcttctaa
     1381 cgtattatca attccttcgg cactcatgtc ggtttccgag tcctctggtt tgtggtgaat
     1441 tcaggcaatc ggctttcacg aaccgattga tcggcgcata cctgtttcat tcggtacatc
     1501 cctgataatt cttaatattt tcgggcttat ttcatgatca tgtttcagaa aaaacccaca
     1561 acgtcttgtt cgtgctcagc gcttcttggt cagcccgcag ttgacctgtt ggaagaaagc
     1621 cttttatggt tgaacgattt cttcagacgc tattcatgga aaatatggcg ccattcgctg
     1681 ttattaccgg agcttcacga gggattggag ccgaatatgc cagagcctta gcggctcaag
     1741 gctaccatct cctgctggtt gcccgggacc ataaccgtct gcaggattta tccaaggaga
     1801 ttcaacagac cacctctatg caagtctgga cagaaacgct ggatttggcc aaacctaacg
     1861 cggctggaac cctttaccaa ttagctcaat catatcggcc gcatgtgtcg ttgcttgtca
     1921 acaatgccgg ctttgggctg tatggcccat ttacaggcat gccactctca acgatccaag
     1981 acatgttaca ggtccatatc catgcgacca cagaaagcac aagactattc ctacctggta
     2041 tgatgaatcg acgggagggt gcgattatca atgtggcttc tgtggcagga tttttcccaa
     2101 ttccctatat ggcggaatac gccgccacca aagcctttat catatctttc tctgaagcgg
     2161 tggcgatgga ggccaaagaa aatgaggtaa ccatccaggt atgctgtcca ggctacacag
     2221 aaaccgattt ccataggacg gcagggcact gcccacgcca catttcttct cctcacgctc
     2281 cctacgacgt cgtacgaaaa tcattaaagg ccctaaagtc ccggcaaacc ctcgtgacta
     2341 ttgggtggca gggactggca accgaatgga taactccatt tattcctaaa aagtggctca
     2401 ggcgtcttgc cagccgattt gttcgaccaa attccccctc tcattgaaca cacggcctcc
     2461 catactaaag gagcacagca tggcccattc ttatttccat tcaattattg tcccgctcag
     2521 catctgtagt gtctgcatcc tagcgtttgg gtgctctggc cctcgcccaa tcctctatcc
     2581 caacaactat cttcaacaag ttggacccga tcgagctgaa caggacattg aggaatgcca
     2641 acagcttgct gaagattatg tcccagaaca cgatgcctcg acggtggccg ggaatactgc
     2701 cgttggggcg ggagcagggg gtgccgtcgg tgcagtcagt ggggccattc gaggaggagt
     2761 gggtattgga gcagccatag gtgccgcaac aggtgcgacc gttggcttta tccgtggctt
     2821 atttcaggcc tcgcaaccaa cacccgccca caaggccttc gttagccgat gtctttcgga
     2881 acgagggtac gaatcgattg gttgggaata gatcagaaaa gaaacatgaa gaaaatgggg
     2941 tgagaacacc ttgagacgct gttcgtggtg gttctcacct tgagaaaatc cagtatcgaa
     3001 acttggctca caggagaatt aagaaaagct taaaatgatg agctttctgc acgacggagg
     3061 gctcgcatca aaactggatc ttgataagcc acttcactta aggcgtccaa gatatgaaga
     3121 tctttttc
//
LOCUS       DATIAL010000470         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1159571, whole genome shotgun sequence.
ACCESSION   DATIAL010000470 DATIAL010000000
VERSION     DATIAL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159571"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1818
                     /locus_tag="VLA60_14495"
     CDS             <1..1818
                     /locus_tag="VLA60_14495"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HSF10616.1"
                     /translation="VAQLELPDSPSKESGAIKAWLRGELKQLATLKKILIFEKVTRSN
                     ARDLIVSLAHWTRLLGKPGMVLTIDISAFTGGKEAGATSLTYSPSAVMDAYEMLRQFI
                     DGSDEIEGLLVVVVTSQAFLSDQRLGLNRYEALKLRIWDDVRDKTRQNPFAPMVRLTD
                     QTAVFAESSLLARVGNRKEDVQHQRVIESLRAGVPSPGVVQALGCPQPMVKSKFQQVL
                     QDAQASIHKGWTTKGMLIEGGFGTGKSHLLESLRRMALESRFVCSKVVISKETPLYSP
                     ALMFRSAIEAADIPEKRGDVLAEVAADLNFKSPQYANFYEWVHRQGGEIDGRFAASVF
                     LYERMVNDPELSHRIIRFWAGDPLTDAEIKRYLKSCDGSVSYRFERVSPMEMALQRFK
                     FVSRLMIAAGYSGWILLIDEAEIIGRYSFKQRAQSYAELARWLGRLEASSFADLGYKS
                     GLAAVMALTDDFQSAILDEKGDREKVPEKLRESGSETDLVLSRQAEQGMRLIECERIS
                     LVGPYDQLVEEIYHKIRAIHGAAYGWEPPQVPTIERLSSTRMREYVKGWITEWDLTRL
                     DPTQKVEIELTEIRQDYSEDSELEGDQDDLLHAALSESN"
     gene            1999..>2420
                     /locus_tag="VLA60_14500"
     CDS             1999..>2420
                     /locus_tag="VLA60_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10617.1"
                     /translation="MELRPQEWFHTLRQEYLQRFIGQGGSSVKFVVTDSDSDRVDIQN
                     QLATLAQQEGYACISVDAKDTKIHMMDKFFHQIARRIPWDDLASQFVRRLLQENGYQI
                     PDNREEFCMTGVARMNERAEPLLRRDLNSWLERAIYRDT"
BASE COUNT          634 a          524 c          653 g          609 t
ORIGIN      
        1 gtggctcagt tggaattgcc ggatagtccc tcgaaagaat caggagcaat taaggcctgg
       61 cttcgcggcg agctgaaaca attggctact ctcaaaaaaa tattgatttt tgaaaaggtc
      121 acccgcagta atgcgcgtga cctcattgtc tccttagccc attggacccg cctgttgggg
      181 aaacccggta tggtcctgac catcgatatt tccgccttta caggcggcaa agaagccggt
      241 gcgacctccc tgacctattc gccctctgcg gttatggatg cctatgagat gctgagacaa
      301 ttcattgatg gcagcgatga aatcgaaggg ctattggtcg tggtcgttac ctcgcaagcc
      361 tttctctcgg atcaacgatt gggtctgaat cggtatgagg ccctaaagct tcgaatttgg
      421 gatgatgtcc gggataaaac ccgtcaaaat ccctttgcgc cgatggtcag gctgaccgac
      481 cagaccgcag tctttgccga gagcagtctt ctggccagag tgggaaaccg aaaagaagat
      541 gtgcagcatc aacgggttat tgaatccttg agggcaggtg tcccgagccc gggagtggtc
      601 caagcgctgg gatgtccaca gccgatggtc aaatccaaat ttcaacaggt gttgcaggac
      661 gctcaggcca gcattcataa gggttggacc acaaaaggga tgttgattga aggaggattt
      721 ggtacgggga aatcacattt gctggaatcc ttaagacgta tggcattgga atccagattt
      781 gtctgtagca aggtcgtaat aagtaaggaa acgcctttgt atagtcccgc attgatgttt
      841 cgatctgcca ttgaggctgc tgatataccc gaaaaacgag gcgatgtctt ggccgaggtg
      901 gcagcggatt taaattttaa gagtccgcaa tacgcgaatt tttatgaatg ggtgcatcgt
      961 cagggcggag aaatcgatgg gcgctttgcg gcctcggtgt ttctgtacga acgcatggtg
     1021 aatgaccccg agttaagtca ccggatcatt cgattttggg caggggatcc gttgaccgat
     1081 gctgaaatca agcgatatct caaatcctgt gatggaagtg tatcttatcg atttgaacga
     1141 gtctcaccca tggagatggc gttgcaacga tttaaatttg tttcacgact tatgattgcg
     1201 gcgggatatt ctggttggat tttactgatc gatgaggcag aaattattgg caggtattcc
     1261 tttaaacaac gagcccagtc ctatgcggaa ttagccaggt ggctgggccg actggaagcc
     1321 tccagctttg ccgatctcgg ctataaatcc ggattggcgg cggtgatggc gctcacggat
     1381 gattttcaaa gtgcaattct ggatgaaaag ggtgaccggg aaaaagtgcc agaaaagctg
     1441 cgagaatcag gatcggaaac cgatttggtg ctgtcccgcc aggcagaaca gggaatgcgg
     1501 ctcattgaat gcgaacgcat atccttggtc ggtccctatg accagcttgt agaagaaatc
     1561 taccataaaa ttcgtgccat tcatggcgcg gcgtatgggt gggaaccacc tcaggtccca
     1621 acgattgagc gattgtccag cacccggatg cgtgaatatg tcaaaggctg gattaccgaa
     1681 tgggatttaa cgcgtcttga tccaacacaa aaagttgaaa ttgaattaac ggaaattcga
     1741 caagattatt cggaagattc tgaattagaa ggggatcagg acgatctgct tcatgccgca
     1801 ctctccgagt cgaactagtt tgggtcttga tcaaaatggc tgtgatcaga aaatttgcac
     1861 ataaaaacca ccagattcga atgaatcttt caaagacggg gcaacacgtt ttttcagcat
     1921 aggtctctgc cttttcgaca gtctgtaaaa cctttctgtg tatgcatgtg actttctaac
     1981 ctgtgaagga tacgagcaat ggagttacga cctcaagaat ggtttcatac gcttcggcaa
     2041 gaataccttc aacgatttat cgggcaagga ggttcatcgg tcaagtttgt ggtaacagat
     2101 tctgactcgg atcgagtcga catccaaaac caattggcta ccttggctca gcaggagggg
     2161 tatgcgtgca tctcagttga tgccaaagac accaaaattc atatgatgga taagtttttt
     2221 catcagattg ctcgtcgcat cccatgggat gatctggcct cacaatttgt tcgtcgactc
     2281 cttcaagaaa atgggtacca gatcccggac aacagggaag agttttgcat gacgggagtc
     2341 gcccggatga atgaacgggc ggaaccgtta ctgcgacgag atttaaatag ttggttagag
     2401 cgggccatct atcgagatac
//
LOCUS       DATIAL010000471         3511 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1483887, whole genome shotgun sequence.
ACCESSION   DATIAL010000471 DATIAL010000000
VERSION     DATIAL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483887"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..388)
                     /locus_tag="VLA60_14505"
     tRNA            complement(314..388)
                     /locus_tag="VLA60_14505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(354..356),aa:Gly,seq:ccc)
     gene            complement(512..1735)
                     /gene="tyrS"
                     /locus_tag="VLA60_14510"
     CDS             complement(512..1735)
                     /gene="tyrS"
                     /locus_tag="VLA60_14510"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HSF10618.1"
                     /translation="MTTSEINRQLDLILRGTVEVIQLDELKAKLTESQKEQRPLKIKA
                     GFDPTAPDLHLGHTVLIQKLKHFQELGHQVIFLIGDFTGMIGDPTGVSETRVALTKEQ
                     VLANAKTYEQQIFKTLDPRKTRIEFNSHWMSGMRVEKMVELCSHYSVARMLERDDFSK
                     RYREQKHISVHEFLYPLVQGYDSVVLEADVELGGTDQKFNLLVGRDLQRDYGQKPQVV
                     LTMPLLEGTDGVRKMSKSFGNYIALEDRPADMFGKLMSISDVLMLRYYELLTVRNLDE
                     VKHQHPMEAKMALAEQIVRQYHGSDAVEEAKADFQQRFQKRDFPDEPDARVKLTPTDF
                     HLSQDYSMPVVDLLMRTGLVPSKSEARRLVSQGGVQVDGEKLADPNGMMTFEPGRSYP
                     MKIGKRKFAIIEFRE"
     gene            complement(1767..2237)
                     /locus_tag="VLA60_14515"
     CDS             complement(1767..2237)
                     /locus_tag="VLA60_14515"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF10619.1"
                     /translation="MKLALLGMGVFFVYWVGWPQPSLSSVAPLEHSGVQPSHTLPSGS
                     ADDPDEVVSVAPLSGRNVGQPLQVGVEGEEGQSREVAAFTVDLNDGTLAELEHLPGIG
                     PILAGRIVAHRTSHGAFRRIEDLALVPGIGKKRLEQLRPLVGVRASTMAIGIGS"
     gene            complement(2362..>3511)
                     /locus_tag="VLA60_14520"
     CDS             complement(2362..>3511)
                     /locus_tag="VLA60_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF10620.1"
                     /translation="VARKEEVVRGLVKGIGILLKQWKITHVPGRGELVNDRQVRVTHL
                     DGSSSTMDADAIILATGSSWPQLPQFPIDGQRVLTSKEALDLTEIPRSMVIVGGGVEG
                     CEFACLLSGLGSEVSVVEMMPSVLPLEDEDTISTMTRELKKRKIQLYLNTQITNWAAV
                     DEGVRATLASGEELVAGHLLVSVGRRLNSGRLGLDQVGVQVGTRGEILVNDKMETSIP
                     GIYAIGDVTGKFMLAHVASAQGKVAASNAMGRPQAINYDVIPAGIFTLPEIGRVGLTE
                     AQARERGLAVAVGRFKYAGLGKAQAVGEPFGHFKVISDDQTKKILGVHIIGAHAADLI
                     HEAAMAMQGGLTVEVLANMIHAHPTFSEGLMEAAEDVEGMAIHQARKR"
BASE COUNT          859 a         1013 c          827 g          812 t
ORIGIN      
        1 tcaggaagtt ctactgtgag ggtgacgagt ggttgagcgg gcagcaatgg tcggggtcat
       61 gaataacaaa aagtctgcct tagagtgatt gacaaaaagc cgagacagat ggccttggaa
      121 gcattggata tcacaatgag agtggaaggc aggatggaca agttcgagca cagaagataa
      181 tgtcccatga cagcctgagt tgatcctgga ccgagtcccc gtccaacatt ttcctgaatc
      241 ccctaccgtg acacaaaccg gaaatgttca gaactttaag cccgaagaag atgagggaag
      301 tggtggattg aattggagcg ggcgagggga tttgaacccc cgacaacttg cttgggaagc
      361 aaggactcta ccactgagct acgcccgctc acgagatcag gattcgcatt ctaggtgtgg
      421 gctgagtctt agtcaaggta caaaaaattt gagagtggct cgattggctc tgccgtcgaa
      481 gccagtccct ccaggatata tccggacgaa tctattccct gaattcaatg atcgcgaatt
      541 ttcgcttgcc aattttcatc ggataggatc ggccaggctc gaaggtcatc atcccgtttg
      601 ggtccgcaag cttttctccg tccacctgga ccccaccctg ggaaacgagt cgtctagctt
      661 cagatttact gggcactaag cccgtccgca tcaggaggtc aacgacaggc atggaataat
      721 cttgcgagag atgaaaatcc gtgggagtca gtttcacccg ggcatccggc tcatcgggaa
      781 aatcccgctt ttgaaatcgc tgctgaaaat cagccttagc ctcttccacc gcatcgcttc
      841 catgatattg acgaacaatc tgttcggcca gggccatttt ggcctccatc ggatgttgat
      901 gcttgacctc atccaaattg cggaccgtca acagttcgta atagcgcagc atcaggacat
      961 cgctaataga cataagctta ccgaacatgt ctgccggacg atcttccaag gcgatataat
     1021 ttccaaagct tttactcatt ttccggaccc catcggtgcc ttctaacaac ggcatggtga
     1081 gcaccacctg cggtttttgg ccgtaatccc gctgcaagtc acgcccgacg aggaggttaa
     1141 atttctgatc ggttccaccc aactccacat cggcttccaa caccaccgag tcgtacccct
     1201 gcaccaaagg ataaagaaat tcatgcacgc taatatgctt ttgctcccgg tatctttttg
     1261 agaaatcgtc ccgctccaac atgcgtgcca cgctataatg ggaacacaac tccaccatct
     1321 tttcgacccg catgccactc atccaatgac tattaaattc gatacgggtt ttccggggat
     1381 ccagggtttt gaaaatctgt tgttcatagg ttttcgcatt ggccaacact tgctctttcg
     1441 tcagcgccac ccgggtttcc gacaccccgg tcggatcgcc gatcatgccg gtaaaatccc
     1501 caataagaaa aattacctga tggcccaact cctgaaagtg cttgagtttt tggattaaca
     1561 cggtatggcc cagatgcagg tccggcgccg taggatcaaa cccggcttta atcttgagag
     1621 gacgctgttc tttttgcgac tccgttaatt tggcttttaa ttcgtcgagc tgaatgacct
     1681 ccacggttcc ccgaagaata aggtccagct gccggttgat ttctgaagtt gtcatcttgt
     1741 aagccacgct gtgtgaaagt gaaaaattaa ctcccgatcc ctatcgccat ggttgaagcg
     1801 cgaacaccaa caagggggcg cagttgctcc aaccgttttt ttccaatacc cggcaccaga
     1861 gccaaatctt caatgcgtcg aaatgctcca tgagacgttc gatgtgccac gattcgtcct
     1921 gccaggattg gtccaatacc aggaagatgc tctaattcag ctaaggtgcc gtcattcagg
     1981 tccacggtaa acgccgcgac ttccctggat tgtccctctt ctccctccac tcccacctga
     2041 agaggctgac ccacatttct ccccgaaagc ggtgccacgc tcacaacctc gtcaggatca
     2101 tccgcggaac cggaaggaag ggtgtgtgat ggctgaaccc ctgaatgttc gaggggagcc
     2161 actgaagaaa gagacggttg cggccaacca acccaataaa cgaaaaacac tcccatcccc
     2221 aacaaggcca atttcaagag gagagagctt ccccaggaaa ttttgtctga atgcctgtca
     2281 ggtacttgca gcgagctggc gtgcgacctc tgcggttcga tgtctttcat gttgaatcaa
     2341 taaacacact tcataacatt atcaccgctt tcgcgcctgg tgaatggcca tgccctccac
     2401 atcctcagct gcctccatca gaccttccga aaacgtcgga tgcgcatgga tcatgttggc
     2461 gagaacctcg acggtcaacc caccctgcat tgccatggcg gcttcatgaa taaggtccgc
     2521 cgcatgggca ccaataatat ggacgccaag aattttcttg gtctggtcat ccgaaatcac
     2581 tttaaaatgt ccaaacggct caccaacggc ctgtgctttt ccgagaccgg catacttgaa
     2641 tcgcccgacc gcgaccgcca acccgcgttc ccgggcctgg gcctcagtca gtccgacacg
     2701 cccgatttcc ggcaaggtaa aaatccctgc cggaatgaca tcatagttaa tggcctgcgg
     2761 ccggcccatc gcgtttgagg ccgccacttt tccttgagcc gaggcgacat gcgccaacat
     2821 gaatttccct gtcacatctc caatcgcgta aataccggga atggaggttt ccattttatc
     2881 gttgaccaga atctcacccc tggttcccac ctgcacgcca acctgatcca gccctaaccg
     2941 gccggagttc agacgacgcc cgaccgaaac cagcaggtgg cctgcaacca gttcttctcc
     3001 tgaagccagc gttgctcgca ctccctcatc aacagccgcc caattggtaa tctgcgtatt
     3061 gagatagagt tgaatcttgc gttttttcaa ctcacgcgtc atggtggaga tcgtatcctc
     3121 atcctcaagt ggcagcacgg acggcatcat ttcgacaaca ctcacttccg atcccagccc
     3181 gctcaacaag caggcaaact cgcacccttc taccccaccg ccgacaatca ccatgctccg
     3241 gggaatctcc gttagatcca gagcttcctt actggtcaac acacgttggc catcaatggg
     3301 aaactgaggc agttgaggcc aggaggaccc tgtggcaaga ataatggcat cggcatccat
     3361 ggtggaagag gatccatcaa gatgggtgac ccgcacttgc cggtcattaa ccaattcgcc
     3421 tcgccccggc acgtgggtaa ttttccactg cttcaataag atgccgattc cctttacgag
     3481 cccacgaacc acttcctcct tacgcgcaac c
//
LOCUS       DATIAL010000472         6375 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1375045, whole genome shotgun sequence.
ACCESSION   DATIAL010000472 DATIAL010000000
VERSION     DATIAL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375045"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="glgP"
                     /locus_tag="VLA60_14525"
     CDS             <1..1281
                     /gene="glgP"
                     /locus_tag="VLA60_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HSF10621.1"
                     /translation="LSEASAQVRLSTVFTTHTPVPAGHDVFSGNLIREHCHWWWEELG
                     LTQEDFMALGRHPELSPDQFHMTTLPIRLASFINGVSKEHEQVSKKMFHILWPERPLH
                     EVPIHSVTNGVHVPTWIAQEMDVLYQTYLGSDWRERCDDPNMWQRMKEVPDEEIWEVR
                     KFLKRKLLSFIRQRARIGWMDGTMEPIQVLASGAFLEPHALTLGFARRFATYKRATLL
                     FSDLERLKQILLNPWRPVQIIFAGKSHPADQAGRELIHRIYQFAKAHHLGGHIAFVED
                     YDMHVAKFLVQGVDVWLNNPRPPLEASGTSGQKAALNGVPNLSVLDGWWKEGYDGSNG
                     WAVPLPEEPLGDWAQDDLDMVGLYTKLENEVIPLYYDRGVDGVPHGWCTVVKNSIRTS
                     APRFSARRMLKEYVKRAYTPLFSEAGKARDAKLA"
     gene            1544..1969
                     /locus_tag="VLA60_14530"
     CDS             1544..1969
                     /locus_tag="VLA60_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10622.1"
                     /translation="MKVLRILILLGIVFGLGYYTGQKPDEVKQRLRDLSGQVLENTIG
                     FDQKTSLQRQMLAAKDGFLEGRAYLLDHHFKEASEEFERALHHLDQAVELDPQGPMAQ
                     KIKIVKQKIREAQQSLAQGHSLPPHLLDDLRQELQSVMP"
     gene            complement(2197..3066)
                     /locus_tag="VLA60_14535"
     CDS             complement(2197..3066)
                     /locus_tag="VLA60_14535"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF10623.1"
                     /translation="MRDDQNNIDDAFSDQTEEFVSLEGKTTLPQDELVDEVSEAITAE
                     ADEETADTVASAETEEGAEQEEELVEEQVKDEIDIQIDLLNDSEWVVRREAVITLGEM
                     GDERCVEPLVRCLRDGDWQVRDAAVEAIAMIGSPAVDLLLRYIRDYDARKSVIKALGK
                     INDERVLDPLISMLHNDEFKDDATWALAELGQPAVGRLLECLKNQDEVIRKQAILALG
                     EIKDASCVDLLIERLQDPDWFIRLSSAAALEKIGDPRGREAIKPLMKDPDLVVRLRIE
                     RMLAAWKKQAVTA"
     gene            complement(3112..3315)
                     /locus_tag="VLA60_14540"
     CDS             complement(3112..3315)
                     /locus_tag="VLA60_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10624.1"
                     /translation="MAKRRSLQEDATSLKAKVTKSLASSDNPEGDSAIRSLRKRLRRV
                     QRKVRTAKRREEHRKSKKVMAEA"
     gene            complement(3512..3973)
                     /locus_tag="VLA60_14545"
     CDS             complement(3512..3973)
                     /locus_tag="VLA60_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10625.1"
                     /translation="MTFSMKVLGTLTTAMFLTTGLAIAGPEKDPIPARVPADQRGDAK
                     KDKSPLYEKAEEASPEIVAEGKALYEGKGTCINCHGKEGNGQGPAGAVLNPGPRDFTN
                     CKFHKKRKDGELMWVIKNGSPGTGMVPLVPATISEEEAWKVIAYERSFCKG"
     gene            complement(4351..5265)
                     /locus_tag="VLA60_14550"
     CDS             complement(4351..5265)
                     /locus_tag="VLA60_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HSF10626.1"
                     /translation="MNTGKAFPIQDNAMETLGGLFRQTRERQRLSLEQIASRTRIQQH
                     HLQALEEEDFASLPAKVFVKGFVRSYARSLGLDEDNAIQLFLTSSSNFYDRTQEEQQH
                     IQVTLQAAHRKRFNWNFVVILFLAIGGILFYLLPEQQEPLPPTPESETPLPINETKKE
                     ALLEPLPPIEVSPDSVSSEAPVPTEVQPPSPLPSGITPSPLPPEPQPIAPTFPASPVP
                     EKTIGTDGTLVLEIEATQLTWVVVQSDDQAPHEALLQPGQKSTWKANTQYLLTLGNAA
                     GVVIRLNGELQGPFGKPGQVVRNIRLKP"
     gene            complement(5330..>6375)
                     /locus_tag="VLA60_14555"
     CDS             complement(5330..>6375)
                     /locus_tag="VLA60_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HSF10627.1"
                     /translation="RIGTRRHHSAQSNQSGLAKAVSASITASFIIDHPTLQKKGLIFM
                     GNLLVVGSVAFDSVRTPFGEASNVLGGSATYFSTSASFFTDVNLIAVVGEDFPKEHLD
                     FLHSRGINLDGLEQRPGKTFRWRGEYSYQLNEAQTLETHLNVLETFRPKIPDSYLTPS
                     ALFLGNIDPELQLDVLNQVKRPSIVACDTMNFWIEGKREALWKVLEHIDILVINDGEA
                     RALGNDANLVQVAKRILSRGPKTLIIKRGEYGVLMFHQQEIFGAPAYPLEAVKDPTGA
                     GDTFAGGFMGYLSATENYSQAGLRQAIIFGSVMASFNVEEFSLDRLRHLTREEIDNRF
                     TSFKHLTHFEDLS"
BASE COUNT         1503 a         1501 c         1644 g         1727 t
ORIGIN      
        1 ctgtcggagg cgtccgctca ggttcgcctg agcactgtat ttacaaccca tactcctgtc
       61 ccggccgggc atgatgtctt ttcgggaaac cttattcgtg agcattgtca ctggtggtgg
      121 gaagagttag gactcacgca ggaagatttt atggcgttgg gtcggcatcc ggaattatca
      181 ccggatcaat tccatatgac gacattgcct atccgtctgg cctcgttcat taatggcgta
      241 agcaaggaac atgaacaggt ctcaaaaaag atgtttcaca ttctgtggcc ggaacggccg
      301 ctgcatgagg tgcccattca tagtgtcact aatggggtgc atgtccccac gtggattgct
      361 caggaaatgg atgtgttgta tcaaacatat ctgggatctg actggcggga acgatgtgac
      421 gacccaaaca tgtggcagag gatgaaggag gtgccggatg aggaaatatg ggaggtccgg
      481 aaatttctga aacgaaagct gctaagcttt atccgtcagc gtgctcggat aggctggatg
      541 gatgggacga tggagcctat ccaggtgttg gcgagcgggg cgtttcttga gccgcatgcg
      601 ttgaccctgg gatttgcgcg acgatttgcg acctacaaac gggccacgtt gttgttttca
      661 gatctggagc ggctgaaaca gattctgttg aatccatggc gtcctgttca aattattttt
      721 gccgggaaat ctcatcccgc cgaccaggca gggcgcgaac tcatccatcg tatttatcaa
      781 tttgccaaag cacatcatct gggcggccat attgcctttg tcgaagatta cgatatgcat
      841 gtggccaaat ttttggtgca aggggtcgat gtctggctca ataatcctcg cccgccttta
      901 gaggcgagcg ggacaagcgg ccagaaagcc gccttaaatg gcgtgcctaa tttgagcgtg
      961 ttagatgggt ggtggaaaga aggctatgat ggaagcaatg gctgggcagt gcctctccct
     1021 gaagagccac ttggtgattg ggcacaggat gacctggata tggtcggcct ctacactaaa
     1081 cttgaaaacg aggtgattcc gttgtattac gaccgtggtg ttgatggcgt tccacatggg
     1141 tggtgtactg tcgtcaaaaa ttccattcgg acatcggctc cacggtttag tgcccgtcgc
     1201 atgctcaaag aatacgtaaa gcgtgcctat acgccgctgt tctcagaggc agggaaggca
     1261 cgagacgcaa agctggcctg aatctctcag cgtgttctat aaatttcggc tcatgtgcgg
     1321 taggctgact ctttgcccag aaggtcatgg agtatcccgt gtcagagttg taccttctgc
     1381 aatgcagtga taccatcgac ttacctatgg tgcatcattc cctgtctcat cggttcctcc
     1441 tcgatcgata tgagtttccc ttccggcctt attcatggag tgaacaaggg gcgatgtcta
     1501 ttcagcgaag agcgggaggc attctgaacc ggcgaggtcc actatgaaag ttttacgaat
     1561 attgatcctg ctgggtattg tatttggatt gggttattac acggggcaaa aaccggacga
     1621 ggtgaagcaa cgattgcgag acttgtcggg acaagtcctg gagaacacga ttggatttga
     1681 tcagaagacc agcttgcaga gacaaatgct ggcagcaaag gatggctttt tggaagggcg
     1741 agcctatctg ttagatcatc atttcaagga agcgtcagag gagtttgagc gtgcacttca
     1801 ccatttagat caagcggttg agttggaccc tcaaggaccc atggctcaaa aaatcaaaat
     1861 cgttaaacag aaaattcgag aggctcagca aagtctggct caaggccata gccttcctcc
     1921 tcacttgttg gacgatcttc gtcaagagtt gcaatccgtt atgccttgat caagcccttc
     1981 aaatcgcccc ccgccgtttg tgttctttgt tggtttcaat cccatcttat tattccggat
     2041 tcgtcctttt ctgatcatcc cttcgtcggt tccttatgtg gaatgtaaga gtgcgggcgg
     2101 gaagatgtga tgggactcat cccataaaaa gagccctcaa ttgtccagac aattgagggc
     2161 tcaggttcgt ttacaaccct gacgctttta acaggattaa gccgtgacag cctgcttttt
     2221 ccatgcggcc aacatgcgtt cgatgcgtaa gcgcacaacc agatcaggat ctttcatcaa
     2281 gggcttgatc gcctcacggc cacgcggatc tccgattttt tccagcgcgg cagccgacga
     2341 gagtcggatg aaccagtcag ggtcttgcaa tcgttcgatc aataaatcca cgcaactggc
     2401 atccttaatc tctccgagag ccaggatggc ctgttttcta atcacttcat cttgattctt
     2461 taggcattcg agtaaccgtc caacagcggg ttgtccaagt tctgcaagcg cccaggtcgc
     2521 atcgtcctta aattcatcat tgtgaagcat cgagattaac ggatcaagaa ctcgttcatc
     2581 gttgatcttc cccaacgctt tgattactga tttcctagca tcgtagtctc gtatatagcg
     2641 gaggagcagg tcaacggccg gtgacccaat catggcaatg gcttcgacgg cggcatcccg
     2701 aacctgccaa tctccgtctc gaaggcaccg aacgagaggc tcgacacagc gttcatcgcc
     2761 catttcgccc agggtaatca cggcttcccg tcggacgacc cattctgaat cattcaagag
     2821 atcgatctga atatcgattt catctttgac ctgctcttcg accaactctt cttcttgttc
     2881 tgcgccttct tcggtttcag cagaggcaac ggtgtcggct gtttcttcat ctgcttctgc
     2941 cgtgatggct tctgagactt catccactag ttcgtcttga ggcagggttg tcttgccttc
     3001 caacgataca aactcctccg tttgatcgga aaatgcatca tcgatattgt tttggtcgtc
     3061 tcgcataact caattcctcc acattactct tcagggtcga tctgcagaac cttaggcctc
     3121 tgccatgact ttctttgatt ttcggtgttc ctcgcgccgc ttagcggttc ggaccttccg
     3181 ttgtacccgt cgtaatcgtt ttcgtaatga ccgaatggct gaatctccct caggattgtc
     3241 gctactggct agggatttcg tgaccttggc ttttagagag gttgcgtctt cctgaagaga
     3301 tcgtcgtttg gccatatgat tatgctctcc ttacactaaa aatttgattg acgaataatg
     3361 gtatgggtta ccttctacca cgttgagtct agtcaaggga tttgaatggt gtcaacgggg
     3421 aaccgtcatt attggcaggg gttaggttct tctcaaaaaa aaacccccgc taggtcaagc
     3481 ccagcggggg ttttcgtgaa aatacctact attaaccctt gcagaaactt ctttcgtacg
     3541 caatgacctt ccaggcctct tcctcactga tggtggctgg gactaaagga accatgcctg
     3601 tgcccgggct accattcttg atgacccaca tgagttcgcc atctttccgt ttcttgtgaa
     3661 atttgcaatt ggtgaaatcg cgtggacccg gatttaagac ggctcctgcc ggaccttggc
     3721 cattgccttc tttgccatgg cagttaatgc aggtcccttt tccctcatac aaagctttcc
     3781 cttccgccac gatttccggc gaagcttctt cagctttttc gtataagggt gatttatctt
     3841 tcttagcatc accacgttga tcagccggaa cacgggctgg aattggatct ttttctggtc
     3901 cggcgatggc caagcccgtg gtcaaaaaca tggcagtggt taaggttcca agcactttca
     3961 tcgaaaacgt catgtaagcc tcctaggtta aacagagttg atgagaccgt aaaaagcatg
     4021 agggggcatt atcgcttccc ctttttcctc ttcagagtgg tgttgtttga gtgcacctaa
     4081 cttattaata atcataacat atttataaga taggttgact ttaagccacc caaactccgt
     4141 catcatatca atcacttttt tagactgtca agaaggtgaa aaatgagttg ttgtggacat
     4201 agattgacga ggcactctgg tatggttttc gtccattgcg ttcaagtgaa aaaggcaaaa
     4261 tattctggag tcggtagtct ttgaccctaa ctaatacttc aaccacaatc ctgaatatta
     4321 tttggagccc ttggcattcg ttttgtgagg tcagggcttc aagcgaatgt ttctgaccac
     4381 ctgacctggc tttccaaatg gaccttgtag ttctccatta aggcggatga ccacaccggc
     4441 agcgttccca agcgttagca agtattgtgt gtttgccttc caggtgcttt tttgtccagg
     4501 ttgtaataag gcctcatgcg gagcctgatc gtcagactgc accaccaccc aggtcagttg
     4561 ggtggcttcg atttccagga ccagcgttcc atcggtccct atggtctttt caggaacggg
     4621 ggaagcaggg aaggttgggg ctattggttg aggctcaggg ggtagcggtg aaggtgtgat
     4681 gccggatggg agaggggaag ggggctggac ctcagtgggg acaggggctt ctgatgaaac
     4741 actgtcgggg gatacttcaa tggggggtag gggctcaagt agggcttcct tcttggtctc
     4801 attgattggt aaaggagttt ctgattcagg ggtgggagga aggggttcct gctgttcggg
     4861 tagaagataa aagaggattc caccgatggc tagaaaaaga atgacaacaa aattccaatt
     4921 aaatcgttta cgatgggctg cttgtaaggt gacttggatg tgttgttgct cttcttgggt
     4981 gcgatcatag aaattgctag aggaggttaa gaaaagctga atggcattat cttcatccag
     5041 tcccaacgaa cgggcatacg accgaacgaa tcctttgacg aacactttgg ccggtaggct
     5101 tgcgaaatcc tcttcctcaa gcgcttgaag gtggtgctgt tgaatgcggg ttctggaagc
     5161 gatctgctcc aaagacaaac gttgtcgttc ccgggtctgt cggaataatc cacccagagt
     5221 ttccatggcg ttatcctgaa tgggaaaggc ctttccggta ttcatgtgat tgacttgtca
     5281 tgggaagaca cacgtcatgt aaaaggtgtg ccttccgaaa tcctgagtcc tatgacaaat
     5341 cctcaaaatg agtcagatgt ttaaacgatg taaatcgatt gtcaatttct tcacgggtta
     5401 aatgcctgag tcggtccagg ctaaattctt ccacattgaa ggacgccatg acacttccaa
     5461 aaatgatggc ctgtcgaagc ccagcttggg agtaattttc cgtagccgaa aggtacccca
     5521 taaagccgcc ggcaaaagtg tcgcctgcgc cggttggatc tttgaccgct tccagcggat
     5581 aggctggagc cccgaaaatt tcctgctgat gaaacatcag gacgccatat tctccgcgtt
     5641 taataatcag ggttttgggg ccgcgcgata agatccgttt cgccacttgt acgagatttg
     5701 catcattgcc caatgcacgg gcctccccgt cattaatgac gaggatatcg atatgttcaa
     5761 ggactttcca taacgcttcc cttttcccct cgatccaaaa gttcatggta tcgcaggcca
     5821 cgatagaggg ccgcttcact tgattcagga catccagttg taattctggg tcgatgttgc
     5881 ccagaaacaa ggcggatggc gtgaggtagc tatcgggaat tttgggtcga aacgtctcta
     5941 gaacattgag atgggtttcc agggtttgag cttcgttgag ttgataggaa tattctcctc
     6001 gccaccgaaa ggttttccca ggtcgttgct ctaatccatc cagattgatg ccccgactgt
     6061 gcaggaaatc gagatgttct tttggaaagt cttcgccaac caccgcaatg agattcacat
     6121 ctgtaaaaaa actggccgaa gtggaaaaat acgtggcgga tcctcccaac acattcgagg
     6181 cttcgccaaa cggcgttcgt acggaatcaa aagcaacgga tccaacgact aataaattac
     6241 ccataaagat taggcccttt ttttggagtg tagggtggtc gataataaat gacgccgtaa
     6301 tagaagctga taccgctttc gcaaggccgg actgattcga ttgagcggag tgatgacggc
     6361 gtgttccaat gcggt
//
LOCUS       DATIAL010000473         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1160248, whole genome shotgun sequence.
ACCESSION   DATIAL010000473 DATIAL010000000
VERSION     DATIAL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160248"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VLA60_14560"
     CDS             <1..945
                     /locus_tag="VLA60_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10628.1"
                     /translation="NLTNTAVFPNSVQTALPYWTKIRHKTEYDAERAGHSSLTPHYQD
                     GDGIIATHSLNNRGSLVFFGFASENGLIPTSFTSANPTPYSPVEIIISQGQVEGATSL
                     IQVEVAHPSGPPLLAPVYANSQVVFAGGTAAVEGFDSCGLLAGGRPPVKLGPAGGLTG
                     TATFTGNPPTPQVGTESLDLVKALDSLKGGAHVISGDLVGVNLGAPGNPALLYAESLA
                     GGFSSRLAAQNLNGYGILLVTGDLNISAPFHWEGLIIVAGQVTFDGGSGSSVIKGALL
                     ADQVQILNGEVKMTLDTCPITASLRVLPVATLSWQQLL"
     gene            1308..>3016
                     /locus_tag="VLA60_14565"
     CDS             1308..>3016
                     /locus_tag="VLA60_14565"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HSF10629.1"
                     /translation="MSVQPDIIYTKVDEAPELASGSFLPIIQSFAKVAGVTVGTKDIS
                     LAGRILAQFPDKLKPEQRQPDDLALLGELVEKPDANVIKLPNISASLPQIKGAIAELQ
                     SQGYAIPDYPEDPKNEEEKDIKAKYDKVKGSAVNPVLRQGNSDRRASASVKQYAKKNP
                     HKMGKWTKDSKTHVAHMTAGDFFGNEKSVTITEKAAGKGKIEFVADNGSVTVVKDKLT
                     LNKGDVLDASKMSCQALRQFFKEQVADAKQKGILWSLHLKATMMKVSDPIMFGHAVKA
                     FFEDVFEKHEKTFAELGANPNNGLGDILAKIKNLPEAKKKEIEADIQACLKNGPALAM
                     VDSDKGITNLHVPSDIIIDASMAAALRTSGKMWGPDGKEHDMKAIIPDRSYAGIYQAV
                     VDFCRENGEFNPSTMGSVPNVGLMAQKAEEYGSHDKTFESTGKGVVRVLDGAENVLLQ
                     HDVEKGDIWRSCYTTDVAIKDWVKLAVNRARASSTPVVFWLNKNRAHDAQVIEKVNLY
                     LKDHDTKGLDIQIMAPVDAMKYSLKRAKDGLDTISATGNVLRDYLTDLFPILELGTSA
                     KMLS"
BASE COUNT          801 a          694 c          778 g          743 t
ORIGIN      
        1 aatctcacca atacggcagt ttttccaaat agtgtgcaaa ctgctctccc ttactggact
       61 aaaattcgtc ataaaaccga gtatgatgcg gaacgggcgg ggcacagttc tctgacccct
      121 cactatcaag atggggatgg aataatcgcg acccattcac taaataaccg gggcagtctg
      181 gtcttctttg gatttgcatc ggaaaatgga ctcatcccca cgtcatttac gtcagcaaat
      241 ccaacaccat attccccggt cgaaatcatc atcagccagg gtcaggtaga aggtgccacg
      301 tccctgattc aagtggaggt tgcgcacccg tctgggcccc cattgttggc acccgtatat
      361 gcgaatagtc aggtcgtatt cgctggcgga actgcagccg tagaaggatt tgatagttgt
      421 gggctcctgg caggaggccg gcccccagtg aagttggggc ctgcaggtgg gttgacagga
      481 accgccactt tcacaggaaa tcctccaacg ccacaagtgg gcaccgaatc cctggattta
      541 gtcaaggctt tagacagcct caaggggggc gcccatgtca tttcaggaga tcttgtcggt
      601 gtgaatcttg gggcgcctgg gaatcctgcc ctgttgtatg ccgaatcgct tgcaggggga
      661 ttttcgagcc gacttgccgc tcagaatctc aatggatatg gcatactttt ggtaacggga
      721 gatctaaaca tttccgcgcc ttttcattgg gaagggctca tcattgtcgc tgggcaagtg
      781 acgtttgatg gtggaagtgg gagttcagtc attaagggtg ccctcttggc ggaccaggtg
      841 cagatactga atggcgaggt gaagatgact ctggatacct gtccgatcac cgcgtccctt
      901 cgagtgcttc cggttgcgac cctcagttgg caacaacttc tctagagtct ctctaagact
      961 tttctatttc tcggctgata aatctcaaaa tattcccttg caagggttcc tctcaataat
     1021 tcctctaact tccagtttct agttgagttt actttttcgg gtaaaatgtt ttcgcaagct
     1081 tcccctaatt ccgcatcttt cctgagactt ttgattcgtg gcgacatggg tcctttgatt
     1141 gattgcagaa gggtgacacc attcttacaa tggttttctt gccacggcat ggggattggt
     1201 ttacagattt cattttaacc gcttgtggag ttccccaatg gcgtgtttga ggcgagttct
     1261 ttccaggaaa aacgtgttct tagttaacct ttaacggggt aaatgccatg agtgtacaac
     1321 cagacattat ttacacgaaa gttgatgaag cgcctgagct ggccagcgga tcgtttcttc
     1381 caatcattca gtcatttgct aaagtggcag gtgtgaccgt tggcaccaaa gatatctccc
     1441 tcgcgggaag aattcttgcg caatttcctg acaagctcaa gcctgagcaa cgacagccgg
     1501 atgatctggc tctattaggc gaactggttg agaagccaga tgccaatgtc attaagttac
     1561 caaacatcag tgcatcgctc ccacaaatta aaggtgcgat tgctgaatta caatctcagg
     1621 gctatgcgat ccctgactat cctgaagatc ctaaaaacga agaagaaaag gacatcaaag
     1681 ctaaatatga caaagtgaaa ggaagtgcgg tcaatccggt gctgcggcaa gggaattccg
     1741 atcgtcgggc ttcagcctcg gtcaagcaat atgccaaaaa gaacccgcat aaaatgggta
     1801 aatggacaaa agattccaaa acgcacgtgg cgcacatgac ggccggagat ttcttcggca
     1861 atgaaaagtc cgtaactata accgagaaag ccgctggaaa gggaaaaatt gaatttgtgg
     1921 cggacaatgg tagcgttacc gtcgtgaaag ataaattaac cctcaacaag ggtgatgtgc
     1981 tggatgccag caagatgagc tgccaggcat tgcggcagtt ctttaaagag caagtggcgg
     2041 atgccaaaca gaagggcatt ttgtggtcgc tacacctgaa ggccaccatg atgaaagttt
     2101 cggatcccat catgtttggt catgctgtga aagcattctt cgaggatgtc tttgaaaaac
     2161 acgaaaaaac gtttgccgaa ttgggtgcca acccgaataa cggattaggt gacatcctgg
     2221 ccaaaattaa aaatttaccg gaggccaaaa agaaggaaat cgaggcagac attcaggcct
     2281 gtctcaaaaa tggcccggca ctcgcgatgg tggattccga caagggcatt accaaccttc
     2341 atgtgccgag tgacatcatc attgatgcat ccatggcagc tgcactgcga acctcgggga
     2401 aaatgtgggg acctgacggc aaagaacatg acatgaaagc catcattcct gaccggagtt
     2461 atgccggtat ctatcaggcc gtagtcgatt tttgtcgcga aaatggcgaa tttaatccgt
     2521 ctaccatggg tagcgtaccc aatgtggggt taatggcaca aaaagccgaa gaatatggat
     2581 cacatgacaa gacatttgaa tccaccggga aaggcgtagt cagggtgctg gatggagcag
     2641 agaatgtctt gctgcaacac gatgtcgaaa aaggcgatat ctggcgcagt tgttatacga
     2701 cggatgttgc cattaaggat tgggtcaagt tggccgtaaa ccgggccagg gcttcttcga
     2761 cgccggtggt attctggctc aataaaaatc gggctcatga cgcccaagtc attgaaaaag
     2821 tgaatctcta ccttaaggat cacgatacca aaggtctgga catacaaatt atggcaccgg
     2881 tggatgccat gaagtactcc cttaaacgtg caaaggacgg cctcgatacc atttcggcta
     2941 ctggaaatgt gcttcgggat tatctcactg acttattccc gattttggag ttggggacga
     3001 gcgccaaaat gctgtc
//
LOCUS       DATIAL010000474         5072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_838276, whole genome shotgun sequence.
ACCESSION   DATIAL010000474 DATIAL010000000
VERSION     DATIAL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838276"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1555
                     /locus_tag="VLA60_14570"
     CDS             128..1555
                     /locus_tag="VLA60_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10630.1"
                     /translation="MKKFTIGVLMALAVFPTFPAFSASGEEALTPAQQEAVRQAVQDY
                     LKGQGSPEAARPPMEQPGTGIAPPTYAAPPTQRPGEPVKETLGGLSQPRTPDKKYGST
                     MQGSGQLIYARPFVSSPKAIVGGYMDIEYFNRKNQGNSYFDQHRLVPFIYGDISDRVK
                     FAAEIEFEHGTNDIKVEFATIDYLVQEPLNLRAGIILVPLGKFNLLHDAPLRDLTERP
                     LVTQRIIPSTLHQPGVGAYGTFYPTALSKLDYEIYVTNGFTNAFGGNGNPNNTSNINQ
                     TNGIRSARSDNTSFDNNNGKSVMGRVAFSPILGVEIGGSGFYGNYGTNKDDKLAIWAL
                     DWTFQRGPFEFIGESAWSYIKDNDINQDGTRNGNPRRMQGYYLQGNYHFLPEWLTKMA
                     PTFFKPEVSTFTAVVRWEQMNLGQDLSGEAGKLGEWQRWTFGLNFRPTEDTVFKTDFQ
                     YTPVGRNGNERIHDTAFVASWATYF"
     gene            1888..2265
                     /locus_tag="VLA60_14575"
     CDS             1888..2265
                     /locus_tag="VLA60_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HSF10631.1"
                     /translation="MACPIDSVWNSSLETLKAYPVTKKDKSNGLIETGWRVEYVEGAK
                     YGLFQREGMGDKERSQLTLTIKPLDSNAVRLQIAERRQHWGFRGGARLYDWYPVEPSQ
                     QAVNHILNNLTEKLEAEGCFIES"
     gene            2247..2936
                     /locus_tag="VLA60_14580"
     CDS             2247..2936
                     /locus_tag="VLA60_14580"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HSF10632.1"
                     /translation="MFHRILNIAIFGSLLLAFTPQPSNAENDQIWDNELKRYLTPQEM
                     VQEDIYLTPEEAAKLTLPDSDSIRLEIITLNENQKRIIEERIGWQFPESTFDCYIGET
                     NGKIDGWAFVQHTIGKHKPMTYMVGVDPDGEITNVEVLVYRESRGSEVGKKRFNYQYQ
                     GKTIDDPIRINRDVINISGATMSVRSMSAGVKRALVLAHELYLQSQSQSPPINTASRT
                     NKNFLESLLGF"
     gene            2954..3466
                     /locus_tag="VLA60_14585"
     CDS             2954..3466
                     /locus_tag="VLA60_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10633.1"
                     /translation="MRNFNARFRLRDTDRHIRLVYTWFLLLMLAGFIFTFFWAHSMTE
                     LSLKGLADHYRGSDATFGEPMPFGQLAETTHFHLFTMPVTFMILVHVLYLTMVSPTLK
                     VVTTWMAFVGVTLDLISPWLIAYISPIFTIAMLSGDILMVVGFLIMFVIPMYEMWWQK
                     SAFMMPDSDD"
     gene            complement(3850..4002)
                     /locus_tag="VLA60_14590"
     CDS             complement(3850..4002)
                     /locus_tag="VLA60_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10634.1"
                     /translation="MQQVKANTGDIWEMSHGKSQNLWFFSQKPALAVHALSQGIISYS
                     FAEWVQ"
     gene            4404..>5072
                     /locus_tag="VLA60_14595"
     CDS             4404..>5072
                     /locus_tag="VLA60_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10635.1"
                     /translation="MNVKNSILFSFAQVFCIAVLTSTLSFFVNAPLVFAQEAEEADPP
                     EVTNGERLFLETRFAQLFKIFLDDGGDVNDPLPQGDPALDKVTNWQLPPDQYANGPFA
                     GQSMNCRSCHFVDELLDVPNYGMNTYSDFARRSPIPAREDGKHTSVRNAPPLVNAALP
                     RQMFFLHADAEFPTMVDLVTGTLTGRNYGWLSDEQETAISHIARIIREDSGTGDLAQE
                     FGGLS"
BASE COUNT         1322 a         1314 c         1132 g         1304 t
ORIGIN      
        1 tgatcctctt atgcgttgtg ctccgattcc cggtttatca tgagttcctt ccattgagga
       61 ccctgagtcc cctacccttt tcctgaggac ctccgcgtcc caatcctata ttaggaggtt
      121 ttcattcatg aagaagttca caatcggggt ccttatggcc cttgctgtgt ttccaacctt
      181 tccagccttc tctgcctccg gtgaagaagc acttacgccc gctcaacagg aagcggttcg
      241 acaagcagtc caagactatc tgaaagggca agggtctcca gaggcagcca ggccaccgat
      301 ggagcaacca gggaccggga ttgccccgcc aacttatgcg gccccaccca ctcaacgccc
      361 aggcgaaccc gttaaggaaa ctttaggagg attatcacaa ccacgtacgc ctgataaaaa
      421 atacggatca accatgcaag ggtccggaca attgatctat gcccggccct tcgtttcttc
      481 ccccaaagcc atcgtcggcg gctacatgga tatcgagtac ttcaatcgca aaaatcaagg
      541 gaacagctat ttcgatcagc atcgattggt acctttcatt tacggggata tcagcgatcg
      601 ggtgaaattc gcggcagaaa ttgaattcga gcatggaacc aacgacatca aagtggaatt
      661 cgcgaccatc gattatctcg tacaagaacc ccttaacttg cgggcgggca tcatcctcgt
      721 cccccttgga aaattcaacc tcctgcatga cgccccactc cgtgacctca ccgaacgccc
      781 tctcgtgacc caacggatca tcccatcaac ccttcaccaa cctggcgttg gagcttacgg
      841 caccttttac cccaccgctc tctcgaagtt ggattacgaa atctacgtga ccaatggatt
      901 cactaacgcc tttggaggaa acggaaatcc taataacacg tcgaatatca accaaacaaa
      961 cggaatacgc agtgcgagat cagataatac gagctttgac aataataacg gcaaatccgt
     1021 catgggacgt gtcgcattca gccctatttt gggagtggaa atcggtggat ctggatttta
     1081 tgggaattat ggcacgaata aggatgataa attggccatt tgggccctgg attggacctt
     1141 ccaacgaggc ccatttgaat ttatcggaga aagtgcttgg tcttacatca aggacaatga
     1201 tatcaatcaa gacggaaccc ggaatggcaa tccacgcaga atgcaaggat attacttaca
     1261 agggaattac catttccttc cggaatggct aaccaaaatg gcccccacct tcttcaagcc
     1321 cgaagtctct accttcaccg ctgtagtccg ctgggaacaa atgaacctgg gacaggattt
     1381 aagcggggaa gccggaaaac tgggggaatg gcaacgttgg acctttggcc tgaacttccg
     1441 tcccacagaa gatactgtgt tcaaaaccga cttccaatat acgcccgtcg gtcgaaatgg
     1501 aaatgagcga attcatgata cggcttttgt cgcatcctgg gccacatatt tctaagaatt
     1561 cgctttgaac aggaaaacag agagaaacgg ctttgccgtt tctctctgtt tttagcttca
     1621 atcagtatcg aatcatatgg accacatcag accatatttt aaagaacata ccggttgacc
     1681 ccttgaacat cggatgatac aattcgttct tatttttcat aatgagaatc aaattcaaat
     1741 aaatcgaaaa tcattctcgc ttttaattta tcaatgttgt atctatctcc cgccttccta
     1801 attcgtggaa tctctatttg ggcatgcaca accttttttc tgggctgcgg tttcctttcc
     1861 ggtcaagact tgattgtcga tcgatacatg gcctgtccaa ttgattccgt atggaattct
     1921 tccctggaaa ctctcaaggc ctatcccgtg actaagaaag acaagtccaa cggattaatc
     1981 gaaaccgggt ggcgggtgga atatgtggaa ggcgccaagt atggcctttt ccaacgggaa
     2041 ggcatggggg acaaagaacg atcgcaactg accctgacca taaagcccct cgattccaat
     2101 gccgtgcgcc tccaaattgc ggaacggcgg caacattggg gattccgtgg cggggccaga
     2161 ctttatgatt ggtatcccgt cgaaccatca caacaagccg tgaatcacat tttaaacaac
     2221 ttaaccgaaa aacttgaggc agaaggatgt ttcatcgaat cttaaacatc gctattttcg
     2281 ggagtctttt actggcgttt accccccaac cctccaatgc cgagaatgac cagatctggg
     2341 ataatgaatt gaagcggtat ttgactccac aggaaatggt tcaggaggac atctacctga
     2401 cgccggaaga ggctgccaaa ttgacacttc ctgattccga ttccatccgc ttagagatca
     2461 tcaccctcaa cgaaaaccag aaacgaatta tcgaagagcg aatcggatgg caatttccgg
     2521 aatcgacctt tgattgctat atcggagaaa cgaatggaaa aatcgatgga tgggcttttg
     2581 ttcaacatac catcggtaag cataaaccga tgacctatat ggttggagtt gaccccgatg
     2641 gagaaatcac caacgttgaa gtattggtct accgggaatc acgggggagc gaagttggaa
     2701 agaaacgctt caattaccaa taccaaggaa aaacaattga cgaccccatt cggattaacc
     2761 gggatgtcat caacatttcc ggcgccacca tgtccgtgcg atccatgagt gccggcgtta
     2821 aacgcgccct cgtcctggcc catgaacttt acctgcaatc ccagagtcaa agtcccccca
     2881 ttaatacggc cagtcgaacg aacaaaaact ttttggagtc cctgctgggg ttctaacctc
     2941 ctgcagcaaa ggaatgcgaa atttcaatgc ccgcttccgg ctccgtgata ccgaccgtca
     3001 tatccgctta gtctatacct ggtttctcct gctgatgctc gcaggattca ttttcacctt
     3061 cttttgggct catagcatga cggagttaag ccttaaaggc cttgccgatc attatcgagg
     3121 atcggatgcg acctttggtg aaccaatgcc ctttggtcaa ctggccgaga ccacacattt
     3181 ccatctgttt accatgcccg tcacattcat gattttagtc catgtgctct atctcacgat
     3241 ggttagcccc acactaaaag tggttacgac atggatggct tttgtcgggg tcaccctgga
     3301 tctgatttca ccttggctga ttgcctatat atccccaatc ttcaccatcg ccatgttgtc
     3361 cggagacatc ctgatggttg tgggctttct catcatgttc gtcataccca tgtatgaaat
     3421 gtggtggcaa aaatccgcct ttatgatgcc tgactcggat gactgacttt tgcccttttc
     3481 ccgattggag gaaaattctt taccaggacc catgcctaga cgcgggtcac ttcaaccacc
     3541 agttggctta aaacggctac cggattttat gatggaatgc gacaagagtt ttctcactca
     3601 atcgaatcag acaaccatac tttcatgaga gaggaacaga ttcttcgtcc atatcctaca
     3661 tcggcctggt caagggttcc ttgttatcat ggctttggga attggatagg catcagcctc
     3721 ttttccatat ttctttctgc ctgcgcacca gatttgtatt gggcaaaacc agacgcaggc
     3781 gagggtgaat tcgaacagga tctgcgctca tgtcgggaaa tcctgatcaa tgaagtacat
     3841 tccgagactt cattgaaccc attcagcaaa ggaataactg atgatgcctt ggctcaatgc
     3901 atgaacagca agggctggtt tttggctgaa aaaccataaa ttctgacttt tgccatgact
     3961 catttcccag atgtcgcccg tgtttgcctt aacttgttgc acggagatct tccaatagac
     4021 agcagatttc caaggatgtt cgtcgcaccg cataaatgaa atgcgctgcc aaatgagggc
     4081 attgatgatg ccagagtctg acgactaagt acgtctcacc atattcctaa ggcccgagac
     4141 tttttgagtc cagagcccat gagctgccat tgtatgctca tgggcttttt ttgtgaacaa
     4201 ggaatgtcat gccctgattc ttcatatatc ccgaggatga atacccttac attcatcctc
     4261 atgtaggcga gggggtgggc ctcctcctgt ccccctcgcc tcgctccgcc tcaacacatg
     4321 aggtgtgaga ttacgttcac cattaatcca taagggatca aagaggattt ctccgaccat
     4381 tcattttaaa gcgaggtgcc cccgtgaacg tcaaaaattc tattcttttc tctttcgctc
     4441 aggtcttttg tattgcagta ctcacctcaa ctctatcctt tttcgtcaat gcacctctgg
     4501 tgttcgctca ggaagccgaa gaagctgatc ctccggaagt caccaacggt gaacgtttgt
     4561 ttctggaaac ccgatttgct caattgttta aaatctttct ggatgacggg ggagatgtga
     4621 atgatccctt gccccaaggg gatcccgctc tcgacaaagt aacgaattgg caactcccac
     4681 ccgatcaata tgccaatggc ccgtttgccg gtcaatcgat gaattgccgg agttgccatt
     4741 ttgtcgatga actcctcgat gttccgaact acggaatgaa tacctattcg gattttgcac
     4801 gacggagccc tattccagcc cgtgaagatg gaaagcatac ttcagtccga aacgcgccac
     4861 cgctcgtcaa tgccgctctt cctcgacaaa tgttttttct acatgccgat gccgaattcc
     4921 cgacaatggt ggatttggtc acaggtacac tcaccggacg gaactatgga tggctttccg
     4981 acgaacagga aaccgccatt tcacatattg ccagaattat tcgagaagac tccggaaccg
     5041 gagatctggc gcaggagttt ggcggactgt cc
//
LOCUS       DATIAL010000475         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_623578, whole genome shotgun sequence.
ACCESSION   DATIAL010000475 DATIAL010000000
VERSION     DATIAL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623578"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1374)
                     /locus_tag="VLA60_14600"
     CDS             complement(409..1374)
                     /locus_tag="VLA60_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HSF10636.1"
                     /translation="MDILQELRRVIRLEGQAIAHLEESIGLPFEEAVKMLQACQGKVI
                     LTGVGKSGLIANKISATMVSTGTPAVFLNGSEGLHGDIGIVAKEDIVIAVGKSGESEE
                     LLALLPFIRKIGARMIAITAQANSTLARSSDLVLVTPIQEEACPLNLAPTCSTTAALV
                     LGDALAMALMKLRNFQPADYAMFHPGGQLGKRLLLNVGDLMRTGEANAVIRLSHTIQF
                     MLCEMTSKRAGAVSVLDEGDRLLGLITDFDIRRVLEGGQNLFALTIGAVMNPKPSWVY
                     QDEKAVKVLQFMEKREKPISVLPVLDRQEKVVGMIHIHDLISRGL"
     gene            1803..>2437
                     /locus_tag="VLA60_14605"
     CDS             1803..>2437
                     /locus_tag="VLA60_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10637.1"
                     /translation="MKTPKLSSATTQEITRMYNALDYDGLGPVYCDEGGDAFWEDRRG
                     PCKKLGIAIARALRSRLPTMGGSLYVGAGVPEIPVLLMERLELNRTVCPYNLRQAEVD
                     ILNQAGGENGIHFYCGSAETATGKVDHLWIVSVLNDPEEFPNLSILFSYGRADPLAFD
                     VSAFSHERETGVRLFANCMAKLTLPGLLTTSVEEIPWVEHWCHSHGIPYQV"
BASE COUNT          668 a          623 c          556 g          590 t
ORIGIN      
        1 agagggaaaa agaaatattc ttgaatatga aacagtcggg gagaaaaccc caaaagaata
       61 actacacaaa gcccccatcc aaggacaggc caaagatcat aactggagaa agtcctcgaa
      121 gaagaggtgc tgccaacctg tcccacgtcc aagtcgatgc tcattggatc aattcatttg
      181 agacaaggta gatctcgcac aagatatctt ttcaaactga ccatcattag tcgttcacag
      241 gtgggaaaaa acatctgaaa ctctctcccc ctacatgcag gcgaacgagc ttcccgtaat
      301 caaggaaatt acatggatgc ttcctatcag gccttatcct aaccatctta aaggacaatc
      361 tcctctcgaa tcagattcaa gccttgtccc atcgaataga agtctttgct aaaggccacg
      421 ggaaatcaga tcatgtatat gtatcatgcc aacgactttt tcctgtcgat caaggacggg
      481 aagcacggag atgggctttt cccgtttctc cataaattga agaactttga cggctttttc
      541 atcctgatag acccaactgg gctttgggtt catgaccgct ccaatcgtga gggcaaaaag
      601 attttgcccc ccctccaaca cccgacgaat atcaaaatcc gtgatcaatc ccaataaacg
      661 atctccctca tcaaggacgg acacggcccc ggcacgctta ctcgtcattt cacacaacat
      721 gaattgaatt gtgtgagaaa ggcgaataac agcattggcc tcaccggttc gcatcagatc
      781 cccaacgttc aaaagcagtc gttttcctaa ctgcccacct gggtgaaaca tggcatagtc
      841 ggctggttga aagtttcgca atttcatcaa agccatggcc agcgcatccc ctaagaccaa
      901 ggcggcagtg gtgctgcagg ttggtgccag attgagcgga caagcttcct cttgtatggg
      961 cgtcacaaga acaagatcgc tacttcgtgc cagagtggag tttgcctgcg ccgtaatggc
     1021 aatcattcgt gcaccaattt ttcgaataaa tggaagcagt gccagcaatt cttcgctttc
     1081 gcctgacttt cctaccgcaa tcactatatc ttctttcgcc acaatcccaa tgtccccatg
     1141 cagcccctcg gagccattta aaaacaccgc aggtgtgccg gtggacacca tcgtagccga
     1201 aattttattc gctatcaggc cggattttcc cacccctgtg agaatcactt ttccctgaca
     1261 ggcttgcaac atctttactg cctcttcgaa aggaaggcct atgctctcct caaggtgcgc
     1321 gatggcttga ccttccagcc tgatcacccg acgaagttcc tgaagaatat ccataagaat
     1381 ctgatctttt gatctctagt aaaatcggct attccggcaa aaatgaaggt gaaattatca
     1441 ttagataaac agctgacatg ggttctgcct cagaaaaggc gcggcttcgt ccagcacctg
     1501 atgagcacct tactggaaaa ccccaacaaa gagatttcct attgacgcac agtaaaaagg
     1561 aatggaaccg gtgataggag gaactcttgc ccctttttgg gaaaagacct ttttccttgg
     1621 aagagcgtct atgcctattc aagtgtatca atacacaaaa tcgcaatttg ggtcaagttg
     1681 agacagtgct ctccttcctg gattggatca ggcaccgccc attcctgtga gggctattat
     1741 ctgcttcgcc gaacctggta caaagaagga aaccgagaca tattgaaagg cccacacaga
     1801 ggatgaaaac tccaaaatta tcatctgcca cgactcaaga aattaccaga atgtataatg
     1861 cgttggacta tgatggactt ggcccggttt attgcgacga aggtggagac gcgttttggg
     1921 aggatcgccg aggcccctgc aagaaactgg gtatcgcgat tgcgagagcc ctccgcagcc
     1981 gcttgccgac tatgggggga agtttatatg tgggcgcagg agtcccagag attccagttt
     2041 tgctcatgga acggctggaa ttaaatcgaa cggtatgtcc ctataatctt cgacaagcgg
     2101 aagtggatat tctcaaccaa gccgggggcg aaaacggaat ccatttttac tgcgggtcgg
     2161 cagagactgc gacgggcaag gttgatcatc tttggattgt cagcgttctg aatgacccgg
     2221 aagaattccc caatctgtca atcctttttt cttacggccg tgcggaccct ctggcatttg
     2281 acgtatcggc cttttcacac gaacgggaaa caggagttcg cttatttgcc aattgcatgg
     2341 ccaaacttac cctgccaggg ttgctcacga cctcggtgga agaaataccc tgggttgaac
     2401 actggtgcca ctcacatggc attccctatc aagttga
//
LOCUS       DATIAL010000476         5262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_409967, whole genome shotgun sequence.
ACCESSION   DATIAL010000476 DATIAL010000000
VERSION     DATIAL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409967"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VLA60_14610"
     CDS             complement(<1..759)
                     /locus_tag="VLA60_14610"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HSF10638.1"
                     /translation="MNEFFSVKVRYHILSLLLVGTWLLPSSAQAGGLFLTEIGTPDVG
                     LAGAGWAARAQDASTLFKNPAGMSLLEGAQFQGGAQLLYGDIGFTSNGKSTPSGGGGG
                     NPVDVFPGASGFYVHPLGEDWRVGFGIFSNFGLGLKYTGEWVGRYYVKDALLAGVNFM
                     PTASYRVNEYLSVGGGVNVMVGAVKQTVGINNLGSANDGQMQIKDQAVGVGGNIGVMF
                     MPTRGTRFGLTYQSPIDLNFSDKPKFSNLGPIGTA"
     gene            complement(831..1160)
                     /locus_tag="VLA60_14615"
     CDS             complement(831..1160)
                     /locus_tag="VLA60_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10639.1"
                     /translation="MKVLPWWSGCLILTLVSCGGGNLQHMDATPYAQMIERPNGECPH
                     TKIEQGIVNKHPDATIMVTVAEITGAKVVTTSQIPVKGNETVYLGCAVLPSGQDAARK
                     ILNVQFE"
     gene            complement(1223..1918)
                     /locus_tag="VLA60_14620"
     CDS             complement(1223..1918)
                     /locus_tag="VLA60_14620"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSF10640.1"
                     /translation="MIRLPLHVVLILLVVLSGCAGTQQARSVGQSGFLGDYSMLKEGE
                     RDESLLIYKNPQADWKKYQKIILDPVTLWVGKDSQLQDVSPEDRQRMADLFWVKLHEA
                     LKSDYEIVTSPGPDVMRLQAAITEAETSNPILDTVSSIVPQMRVLTGVKGLATGVSGF
                     TGSASVEMKVTDSSDKTLLAAAVDRRGGTKTLRGVTNSWNDVEEAYRYWAEKIRWRAC
                     LLRGGENCVEPEA"
     gene            complement(2171..4033)
                     /locus_tag="VLA60_14625"
     CDS             complement(2171..4033)
                     /locus_tag="VLA60_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10641.1"
                     /translation="MASKQPHCIWFFVILSVAVLAGCQTPVGVARVDSETVRHQLTKN
                     VISSGETSPFTDNVLRVTDLTDAYFDDPRGALNTLHHFFLAEEHRKVYVAFALAELSF
                     LYAEKTNQPSYYLAAALYAYLYLFPENSTTLLNPLDPRGRIAADMYNRGLTSAFITKD
                     RSVMNLQSGSFALPFGTLQVDLPDNRLQWENRKLTNFIPVAELEVFGLHNRYRQAGIG
                     VPLAADQIPLGQEEGLQIAKGKMPVTAFMRFTNLLEQLKSDSIRSDWELYNAYDQKTV
                     NIDGRDVPLEIEFTSSLAASLAESPVWERELKGFFMGESVTLQTGELAALEPYRPGRI
                     PVVFVHGTASGPGRWADMTNDLIIDPSIRNRFQFWYFTYDTGNPILYSAALLRELLQK
                     TVHQLRQKYQDPALDAMVLIGHSQGGLLAKLTAVDSGNTFWNAISNSPLETMKLSDQT
                     RDLLQRSLFIESLPFINRLVFIATPQHGSYVAGSWGAHQLAKFVKLPGRLMSGITDLM
                     TLKLDTFKLNLQNLGSVSGMEPGSPLMTVLPQTPLAPGISGHSIIAVEGDGPPEEGND
                     GVVAYQSAHLDGMESEFVVRSGHSCQSNTHTIQEVRRILLLHAMETCKTHGLCV"
     gene            complement(4253..4726)
                     /locus_tag="VLA60_14630"
     CDS             complement(4253..4726)
                     /locus_tag="VLA60_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10642.1"
                     /translation="MKPFIMFTVAAVVMCVTPAFAENAQITNMQILIDKVKADKKLLI
                     ASNMDLTDKESKDFWPLYEGYQKELEQLNRHLAGLINEYAEAYNKGSVPEKVAKDLIN
                     ESIKTEGAEVKMRREYAEKLSKVLPAQKVARYLQMENKIRAAIKFDLAANIPLMD"
     gene            complement(4948..>5262)
                     /locus_tag="VLA60_14635"
     CDS             complement(4948..>5262)
                     /locus_tag="VLA60_14635"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HSF10643.1"
                     /translation="KRVLLEYLKTINLLRDQSQFYNTLTANCTNVIWMHTRLNPGHVP
                     FSWKILLSGYTPAYLHEQGKLGINIPFEELQRRSHINERARQADQAADFSSRIRAHLP
                     SP"
BASE COUNT         1322 a         1388 c         1296 g         1256 t
ORIGIN      
        1 ggcggtaccg ataggaccaa gattcgaaaa cttcggctta tcggaaaaat tcaagtcgat
       61 gggcgattga tacgtgaggc cgaagcgtgt cccccgagtc ggcatgaaca tgacaccgat
      121 attgcctccc accccgacag cctgatcttt gatttgcatt tgcccgtcgt tggcactccc
      181 gagattgttg ataccaacgg tttgtttgac tgcgccaacc atcacgttga cccctccgcc
      241 gacagacagg tattcattca cccgatagct tgcagtgggc atgaaattga ctccggccag
      301 cagcgcatct ttcacgtaat agcgaccgac ccattccccg gtgtatttca atccaagccc
      361 gaaattggaa aatatgccaa agcccactct ccaatcctct ccgaggggat gcacgtaaaa
      421 tccgctggct ccaggaaaca catcgacggg attcccgccg ccgccgccgg atggagtgga
      481 ttttccgttg gaggtaaacc caatatcgcc atataacagt tgagcgccac cctgaaattg
      541 agcgccctcc aggagggaca tgccggcagg attcttaaat aaggttgagg catcctgtgc
      601 gcgggccgcc cagcccgctc cagcgagccc cacatccggc gtgccgatct cggtcaggaa
      661 gagccccccg gcttgagccg atgaaggcag gagccatgtg cctaccaaaa gtaaactcag
      721 gatatgatat cggactttca ccgaaaaaaa ctcattcatg cttcttcctt atctgaagga
      781 tgccctgagg gaatgtccag ggccacgctt cgaacatggc cctgaatcgc ttattcaaat
      841 tggacgttga gaatctttcg agcggcatcc tggccggacg gcaggacggc gcacccgaga
      901 tacaccgttt cattcccttt cacaggtatt tgggaggtcg tgaccacctt ggctcctgta
      961 atctcggcaa cggtcaccat gatggttgca tccggatgct tgttgacaat accctgttca
     1021 atcttggtat gcggacactc cccgttggga cgttcgatca tttgcgcata aggtgtggca
     1081 tccatatgct ggagattccc tccaccacag gacaccaagg tgagaatcag gcaaccggac
     1141 caccagggca atactttcat agtcatctcc ttttcagtga acaacgcgat gatctgctcg
     1201 gatcaagctg ggatgccggc cattaggcct ccggctccac gcaattttct ccacctcgaa
     1261 gcagacacgc ccgccagcgt attttttccg cccaataccg gtaggcttcc tcgacatcgt
     1321 tccatgaatt ggtgactccc ctcagagttt tggtccctcc ccggcgatcc actgcggcgg
     1381 ccaacagggt tttgtctgag gaatccgtca ctttcatctc cacactcgcc gaaccggtaa
     1441 aaccggaaac gcctgtagcc aaacctttca ccccggttag gactctcatc tgaggcacga
     1501 tgcttgaaac cgtatctaaa atgggattgg aagtttcggc ttccgtgatg gcggcctgca
     1561 aacgcatgac atccggaccg ggtgaggtca cgatttcata atcggatttt aaggcttcgt
     1621 gcaatttgac ccagaacaga tccgccatcc gttgacgatc ttccggggaa acgtcttgta
     1681 attgggagtc cttaccgacc cagagagtca cgggatcaag aatgattttc tggtatttct
     1741 tccaatcggc ttgaggattt ttatagatca gaagggattc gtccctttcc ccttctttta
     1801 acatggaata gtctccgaga aatcctgatt gtcccacgct ccgggcttgt tgggtgccgg
     1861 cacagcccga taaaaccacc aacagaatga gaaccacatg taatggtaaa cgaatcatta
     1921 cgctcctccc ctctcttaag tttgataaga tgattgaaaa tggcagttct taatcgggat
     1981 tatacctata ttcagaatac aaaaaatacg agggataaca ggtacggtta acatggtgta
     2041 cctgtcgtaa ctgaaaaact cttcgcgcat cagtagactc gaaaagtgtt ggtttaagaa
     2101 gatgccaggg ctcaggaaaa taccatcatg ccgtcatgcc atcctcccac caccccttgg
     2161 cacaaaaaca tcagacacag aggccatggg tcttgcatgt ttccatggca tgaagcaata
     2221 aaatccgtcg gacttcctgg atggtatgag tattcgattg acaggaatgc cctgaccgga
     2281 cgacaaattc cgactccatt ccgtccaggt gtgcactctg ataggccaca acaccatcgt
     2341 tcccttcctc cggtgggccg tcaccttcca ccgcaataat tgagtgcccg gaaattccgg
     2401 gggccagagg cgtctgtggc aggactgtca tgagagggct tcccggttcc atgccagaca
     2461 cacttcccaa attttggaga ttcaacttga aggtatccaa tttgagcgtc atgagatcgg
     2521 tgattcctga catcaaccgg cctggcagct tcacgaactt ggccaattga tgtgctcccc
     2581 agcttccggc cacgtaactc ccatgctgtg gcgtggcgat aaagacgagg cgattgataa
     2641 atggaaggga ttcaataaac agactccttt gtaataaatc acgggtttgg tcggagagct
     2701 tcatggtctc cagtggggag ttactgatcg cattccaaaa ggtattcccg ctgtcgacag
     2761 cggtgagttt cgcgagcaat cctccttggc tgtgcccgat caggaccatg gcatcgaggg
     2821 cgggatcttg atatttctgt cgcagttggt ggacggtctt ttgcaggagc tcccggagta
     2881 acgccgcaga gtagagaatg gggttccctg tatcataggt aaaataccaa aattgaaaac
     2941 gattacgaat ggagggatcg atgatcaaat cattggtcat gtcagcccac cttccggggc
     3001 cggaagcggt cccatggaca aacaccaccg gaatacgccc cggcctgtac ggctcaagag
     3061 ccgctaactc tccggtctga agcgtcaccg actcacccat gaaaaaccct ttcagttccc
     3121 tttcccagac cggggattct gccagactgg ccgccagaga ggaggtaaac tcaatttcca
     3181 acggcacatc ccttccatca atattcacgg tcttctgatc ataggcgtta tacagctccc
     3241 agtcactccg aatggaatcg cttttcaatt gttcaaggag attggtgaac cgcataaagg
     3301 ccgtgacagg cattttgccc ttggcaatct gcaatccttc ctcctgacct aagggaattt
     3361 gatcggccgc taacggaacg ccgatacccg cctgccgata ccggttatgt aatccaaaaa
     3421 cctccaactc tgcgacgggg atgaaattgg tgagtttccg gttttcccat tgaagccgat
     3481 tatccggaag gtccacctgg agggttccga agggaagggc aaaggatccc gattgcagat
     3541 tcatgacgga acgatctttt gtaataaacg ctgaagtcag gccccgatta tacatatcag
     3601 ccgcaatccg cccccgggga tctaatgggt tgagaagagt ggtcgaattc tcgggaaaca
     3661 aatacagata cgcatacaaa gcggcggcca gataatagga gggttggttg gttttttctg
     3721 catacagaaa cgacaattca gcaagcgcga acgccacgta gactttccga tgctcttcag
     3781 cgaggaagaa atggtgcaag gtgttcaacg cccccctagg atcatcaaaa taggcgtcag
     3841 tcaggtcggt gacacgaagc acgttatcag taaaggggct ggtttcccct gaagagatca
     3901 cgtttttagt taattgatgc cgaacggtct cggaatcgac ccgggccaca cccaccgggg
     3961 tctgacaccc ggccagaacg gcgacggaaa ggattacgaa aaaccatatg cagtgaggtt
     4021 gtttactcgc catcttcctt agccattctt ttcagaaaat tggatgaaga caaattccga
     4081 ttcatcttca atccaggtat ttcccggatt ggttcacatg gtttctttcc gatgacgagc
     4141 tcacccccac ccttccagga ggagcgagcc tgcccagact gttaaaaacc gagagactca
     4201 acaaatacga tcccggtgat gcacaaatca aaaaacattt ctgaatcctc gactaatcca
     4261 tcaacggaat attggccgcg agatcaaact tgatcgcagc ccggatttta ttctccattt
     4321 gcaggtaacg cgcgactttt tgagcgggaa ggaccttgct gagtttttcg gcatactccc
     4381 tccgcatttt aacttccgcc ccttcggttt tgattgattc gttgatgaga tctttggcca
     4441 ctttttcagg cacggaccct ttattgtagg cttccgcata ttcgttgatg agtccggcca
     4501 gatgacgatt taactgctcc agttcttttt gataaccctc gtagagcggc cagaaatcct
     4561 tggattcttt gtccgtcaaa tccatattgc tggcgataag gagtttctta tcggctttga
     4621 ccttatcgat cagaatttgc atattggtga tttgtgcatt ctccgcaaaa gctggagtca
     4681 cgcacatgac gacggcggcg acggtaaaca tgatgaacgg cttcatacga taacacccct
     4741 tggttataga tagatgatga atgcgaagcg ataagaacgg tactccctca tacttttcgc
     4801 cttcattggc ggcaggaagc gtagcaaagg ccataattaa agtaaacgct gaaggcagat
     4861 cgatccaacc tgtcggtagg aacggttaga actgacctcg cacatgggct gcaaatcgag
     4921 ccccgactag aagcccgggt agggatatca gggagaggga agatgggcgc gtatgcgact
     4981 ggaaaaatca gcagcctgat cggcttgccg tgccctttca ttgatgtggg aacgacgctg
     5041 caattcttca aaaggaatat tgatgcccaa ttttccctgc tcatggagat aggcaggagt
     5101 atacccgctt aacaggatct tccaggaaaa tggcacatgg ccgggattca aacgcgtgtg
     5161 catccaaatg acgttcgtgc agttagcggt cagagtatta taaaattgcg actgatcacg
     5221 cagtaggttg atcgtcttca ggtattcgag cagcacacgc tt
//
LOCUS       DATIAL010000477         6853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1592196, whole genome shotgun sequence.
ACCESSION   DATIAL010000477 DATIAL010000000
VERSION     DATIAL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592196"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1101
                     /locus_tag="VLA60_14640"
     CDS             340..1101
                     /locus_tag="VLA60_14640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10644.1"
                     /translation="MHTRSASQASSHCQSILLIFLAMWCVGPPPSIYPKTLQGVPAGP
                     SSSETCSAHAPVTNECVEAGLRRKRDKLRAEAAFQVNSPTTHLKLAEILNQQGDPNGA
                     IDEYRAAIQLDPDLAGAFQGMGAVYLDQHEWKQAEEVLERAAQLDSGNSQTRYWLGRS
                     RLAQQKFHGAQQAFMTAAKLNPQDAEAFSELGLAFMAQGQSTNAINALQQAIGLRPDY
                     SEAHSRLELVVALKLNQDQLVIEADKILQLLFRRE"
     gene            complement(1180..2049)
                     /locus_tag="VLA60_14645"
     CDS             complement(1180..2049)
                     /locus_tag="VLA60_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HSF10645.1"
                     /translation="MRVSCFSNINRIGRSPPGVLGWSLCGVLLGQTWCFGQETNLEPT
                     PSRPWIMGQAGAFTQPIETDRPTFSLSPNTIAKGRIQIETGYTFSFERASPDVHTHNF
                     PETLVRIGLTERTEFRLEWPNLTFIDNGRDVHGFNDLALGFKVQVWQQQGLRPRLSFV
                     GRLSIPTGDKNFSSDKLDPIFRTILTYTLNERVGLFGTVNIGSPTSQGTRFVQVSSSL
                     GLSAALRDRLTGFVEYFGLYPRDVASGSANYLQTGVLYRLAYHLQLDARVGGGLNHGT
                     DDFLTGAGISWRF"
     gene            complement(2293..5466)
                     /locus_tag="VLA60_14650"
     CDS             complement(2293..5466)
                     /locus_tag="VLA60_14650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF10646.1"
                     /translation="MTQVLNGETLCGKKVFLGKQDGTFAPVMFDARPILTEGLGILGA
                     IVTLKDSDSQNRAERILQSHNEVLEQIISGNSLPEILNNLCLEIERHCGSDTLASILL
                     LNEEGCHLHHGAGLSLPEAYRQGIDGVAIGPEVGACGAAAYLKERIIIPNISTHPNWV
                     RYKDLAERHGLRSCWSMPIMASDGRVLGTFALYYTVLRSPTDEEKEYVEFLAHSASIA
                     IEKVEGQERLRQQTHALEVVNRIGRILAGELDLEKLVQSVTDECRELTGGEFGAFFYN
                     VMDEKGESFVLYSISGAPREAFERFPMPRNIPIFAPTFSGEAVVRIADVKDDPRYGQM
                     ALYHGMPPGHLPVRSYLAVPVVSRSGEVLGGLFFGHSQTNVFTKMAETMVVGIASQAA
                     ISIDNARLYQSAQKEIEQRKRSAENLRRLASIVESSQDAIYSINTDGMISSWNHGAER
                     MYGYMASEMLGQSLSHLLLPEAKQEASWLLDILGTKNGESKRVFLRMKKDGSVFSVSL
                     TISPIVGVDGLLTGHSLIERDISEQQRIGAALEASEGRLQAIWENSPTVKFVKDLDGK
                     YQLVNKRFETIFHLSRGAIIGKTDQELLNPHLASQIIANDQKVLQSGTSQEFEEQLQL
                     PDGIQTYLSVKFPLFDAAGTPYAICGIGTDITARKQAERALQELNDSLEQRVSDRTRD
                     LLVYQENLRAMTSELVVTEQRERRRLSTELHDYLAQLLVVCRMKLAQAINEEKAAGLK
                     TNLEEIDQILSDSLSYTRTLIAELSPTILYELGLVPALKWLGQQMERHGLRVQVQHGE
                     QAIELSEDQAIFVFQAVRELLFNIIKHSGVSEAIVSLERHADQDLKILVQDAGKGFHP
                     SPISTDYSKPGRFGLFSIRERTEALGGHFHIESAPGKGTQATLVVPLQPVSPTVVSNS
                     DSKDIQKTLDPVAQGRSRKKVVRILLVDDHAMIREGIRTLLEHQEDFVIVGEAKNGEE
                     ALEATKLVLPDVVVMDVNMPRINGIEATKFLTNEHPTVRVIGLSVHEDKQIEKMLLEA
                     GAAMYVTKGSVASQLVDAIRQVVNQTC"
BASE COUNT         1793 a         1757 c         1571 g         1732 t
ORIGIN      
        1 aaaaaatgac gacaaaattt aacacaatcc ctttataaat aatacagatt ggcgacaagc
       61 ttcaagattc caacagcgac ggttggtggg tgatttccct aaacacatca atttgtgacc
      121 acatctcaaa tccttttcac cgttaggcag tcacttcatg cgtcactttg aggagcttgg
      181 aagacacctt atcagttggt ttggacatag aagaatggac ggaatagacc gaagccaggc
      241 taatttgtcg aatattccga taaattcttc cccattccgc ctataccgtc cgtctcattt
      301 caaagaacct ttgcccttca tcccctagtg gtatggcaca tgcatactcg ttcagcttct
      361 caagcgtcca gccattgcca atccatcctc ctgatttttc tcgcgatgtg gtgtgtaggt
      421 cctccacctt ccatttatcc gaaaaccctc caaggcgtcc ctgctggtcc atcatcgtca
      481 gagacctgct ccgcccacgc accggtcacg aacgaatgtg tcgaagccgg actccggagg
      541 aaacgggaca agctcagagc ggaagccgca ttccaggtca attcccccac cactcatctc
      601 aaattggcgg aaatactcaa ccagcagggc gatccgaatg gcgccattga tgaatatcgg
      661 gcagcaattc aacttgatcc cgatctcgcc ggtgcgtttc agggcatggg agccgtctac
      721 cttgatcaac acgaatggaa gcaggctgaa gaagtgcttg aacgagccgc tcagttagat
      781 agcgggaata gccaaacgag atactggctg ggtcgatcac gtttggctca acaaaaattt
      841 cacggggctc agcaggcatt catgaccgcc gcaaaactga atccccaaga tgcggaggcc
      901 ttttccgaat taggcctggc cttcatggct caaggtcaat ccacaaatgc catcaacgct
      961 cttcaacaag ccattggctt gcgaccggat tattcagagg cccattcccg cctggagcta
     1021 gtggttgccc ttaaactcaa tcaggatcaa ctcgtcattg aggccgataa aattttacaa
     1081 cttctcttca gaagagaata atatagatat cattcttccc tgagttcagc tctatgtcag
     1141 gtgaaggaac gacacctttg agcgtcgttt cccatccggc taaaacctcc acgagattcc
     1201 cgcacccgtc aagaaatcat ccgttccatg attcaggccg cctcccacgc gggcatccag
     1261 ttgaagatga tatgccagac gatagagcac tcccgtttga agataattgg cgctcccact
     1321 cgccacatct ctgggataca atccgaaata ttccacaaac cccgttaagc gatctcgaag
     1381 ggctgcgctg aggcccagag aggaggacac ctgcacaaac cgcgttccct gagaagtggg
     1441 acttccgata ttcaccgtcc cgaacaaccc gactctttca ttcaacgtat acgtcaaaat
     1501 cgtccggaat atcgggtcaa gtttatccga agagaaattc ttatctcccg tgggaatcga
     1561 cagtcggccg acaaaactca accggggtct gagcccttgt tgctgccaga cctggacttt
     1621 aaatcccagg gcaagatcat tgaaaccatg cacatccctg ccattgtcaa tgaaggtcag
     1681 attgggccat tccaggcgaa attcagtcct ctccgtcaat ccaatgcgga caagggtctc
     1741 agggaagtta tgcgtgtgga catcaggaga ggctcgctca aaagaaaagg tatatcccgt
     1801 ttcgatttga atgcgtccct tggcgatcgt gttaggactc aggctgaatg tcggccgatc
     1861 cgtctcaatc ggctgggtaa aagcgccggc ctgccccatg atccacggac gggagggagt
     1921 aggctcaaga ttggtttcct gcccgaaaca ccaggtctgt cccagcagga ccccacacaa
     1981 cgaccaccct aatacgccag gaggcgatcg tccaattctg ttgatgttgg aaaaacagga
     2041 tacgcgcata gaaattcgaa actatttaaa attaaaaggc tcataaacgt accgtgatgc
     2101 ccttgaaggt cgtcgttcag ggagtgatga gatggggcca ccttttatac caggtaggaa
     2161 ccggaggctc atctatgcga tcacacgata gtatgcgaga acattatcca aggatcaatt
     2221 cggctttgac tgtcagattt tgccttaatg aaacccgtct ataatgattc ccggaaggca
     2281 ttacgagaag gcttagcaag tttgattcac gacctgtcgg atggcatcga ccagttggct
     2341 ggcgacactg cctttggtga catacatcgc ggccccggct tccaagagca ttttttcgat
     2401 ttgtttatcc tcatgaaccg acaatccgat gaccctgaca gtaggatgct cgtttgtaag
     2461 aaatttggtc gcttcgatgc cattgatccg tggcatgttg acatccatca ccacaacatc
     2521 gggaagaacc agtttggtcg cctccaacgc ttcttcccca ttttttgctt ctcccacgat
     2581 gacaaaatct tcctgatgtt ccaggagggt tcggatgcct tcacgaatca tggcatgatc
     2641 atcgaccaaa agaattcgaa cgactttttt ccttgaacga ccttgcgcga ctggatcgag
     2701 ggttttttga atgtctttgg aatctgagtt cgacacaacc gttggtgaga ccggctgaag
     2761 cggaaccacg agggtagcct gagtgccctt ccctggtgcg gattcgatgt gaaaatgacc
     2821 gcccagcgcc tccgttcgtt ctcgaatact aaacagccca aatcttccgg gtttggaata
     2881 atctgttgaa atcggcgatg ggtgaaaccc cttcccggca tcctgaacca ggatcttaag
     2941 gtcctggtcg gcatgacgct caagagaaac gatggcctcg ctcacaccag agtgcttgat
     3001 gatattaaaa agcaattcgc gaacggcctg aaacacaaag atagcctgat cctctgaaag
     3061 ctctatggcc tgctctccat gctgtacctg tacgcgcagg ccatggcgct ccatctgctg
     3121 acccaaccat ttcagagcgg gaacaagccc gagttcgtag agaatggtgg gactcagctc
     3181 ggcaatcaag gtgcgggtat aggaaagcga gtcggaaagg atttgatcga tttcctccag
     3241 attggttttc aagccggcag ccttctcctc atttatagcc tgagccagtt tcatccgaca
     3301 gaccaccagc aattgcgcca gataatcatg cagttcggtg gacaagcgtc gccgttcccg
     3361 ttgttccgtt accaccaact cggaagtcat ggctcgcagg ttctcctgat agaccaacag
     3421 atcacgagtc cgatcagaca cccgttgctc cagggaatcg tttaattcct gcagagcccg
     3481 ttctgcctgt tttctcgccg taatatcggt gcctattcca caaatggcat agggcgttcc
     3541 tgctgcgtcg aataaaggaa acttcaccga aaggtacgtc tgaatcccat caggaagttg
     3601 caactgctct tcaaattctt gcgatgttcc ggattgaaga actttctgat cattggcaat
     3661 aatttggctt gctaaatggg gattaagcaa ttcctgatct gtttttccaa taatggctcc
     3721 cctggataag tgaaaaatcg tttcaaaccg tttattcacc aattgatact tcccatcgag
     3781 atctttcaca aacttcacag ttggagaatt ctcccagata gcctggagcc ggccttcact
     3841 agcctccaga gcggcaccaa ttcgttgttg ttcggaaata tcccgttcaa tgagagaatg
     3901 tccggtcagc aatccatcca cgccgacaat aggagaaatg gttaaggaaa cagaaaagac
     3961 cgaaccatcc tttttcattc tgaggaatac ccgtttcgat tccccatttt tggttcccag
     4021 gatatccaga agccaggagg cttcctgttt ggcttcagga aggaggagat gggatagaga
     4081 ctgaccaagc atttcggagg ccatgtaccc gtacatgcgc tccgctccat ggttccaact
     4141 gctaatcatt ccgtcggtat tgatgctata aatggcatcc tgagatgatt caacgattga
     4201 ggccaatcgt ctcaaatttt ccgcgctgcg ttttcgctgc tcgatttctt tctgagcgga
     4261 ttggtacaag cgcgcattat caatggaaat ggcagcttgc gaggcaatcc caaccaccat
     4321 ggtttcggcc attttggtaa acacattggt ctgtgaatgg ccgaaaaata atcccccaag
     4381 gacttccccc gaccgggata ccaccggaac cgcaaggtag cttctgacgg gaagatgacc
     4441 cggaggcatg ccgtgataga gagccatctg cccataacga ggatcgtcct tcacatcagc
     4501 aattcggacg acggcctccc cggaaaaagt cggggcgaag atcggtatat ttctgggcat
     4561 cgggaatcgt tcaaaggctt cgcgaggggc gccggaaatc gaatacagga caaaagactc
     4621 tcctttttca tccatcacat tataaaaaaa cgccccgaat tcccctcccg ttaattcacg
     4681 gcactcatcc gtgacagact ggaccagttt ctctaaatcc aactccccgg ccaatatcct
     4741 gccaattcga ttcaccacct ccaaagcatg tgtctgttgt cgaagccgtt cctgaccttc
     4801 aactttttca atggcaatgg aggcggaatg cgcaagaaat tccacatatt ctttttcctc
     4861 atccgtcggt gaacggagaa ccgtataata cagggcgaag gtgccaagca cacgaccgtc
     4921 gctggccatt attggcattg accagcatga ccgtaatcca tggcgctccg ccaaatcctt
     4981 gtaacggacc caattgggat gtgtgctgat gttggggatg atgatccgct ccttcaggta
     5041 ggcggcggcg ccacaggcac cgacttccgg gcctattgcc acaccatcaa tcccctgccg
     5101 ataagcttca gggagggaga gacctgcacc atggtgcaaa tggcagcctt cctcattcag
     5161 cagaagtatg gatgccagcg tatctgaacc acaatgtcgc tcaatttcca gacagagatt
     5221 gttcagaatc tccggcagag aattaccact gataatttgc tccagcacct cattgtgact
     5281 ctgcaatatt cgttcggcac gattctgaga atcgctatcc ttcaatgtca cgatagcgcc
     5341 aagaatcccc aggccttcgg ttagtatggg gcgggcatcg aacatgaccg gcgcaaacgt
     5401 tccatcctgt tttccgagga agactttttt accgcaaagg gtttctccgt ttaaaacctg
     5461 cgtcatgaat gttccactga gatttgaaga agagaggtct gaaggctgct ttctcctgtt
     5521 ggaatgggaa agatgaaaaa tttcttcaaa gggcctgttc aagcacagac tttgagcgta
     5581 atgtttgacg gtggagctgg tacgaatgat gtttcctgat acatcacaaa cgatcagagc
     5641 ctctctcgtt tgatccagga cttcacaaag taattgttct tcaaaaccac ccccgccgtt
     5701 ttgctggagc tcaggtccaa cctgacccat agtcggatcg gcagacctga cttcaggcgt
     5761 ccttttagac tcttggaaag gaatgttgga attttccatc ctggtccaat ttcaggtgaa
     5821 catactattc agtcgaatcc ctagggaccc caggttttat atatttacgt aagtcaatca
     5881 tcgttttgaa gcttcttctg aatttttggc atgtaggcac accaaccctc ttccctcaac
     5941 caggatcaaa agacagcctt aaaatgagat gtcattgttt caattttcct atggaagtta
     6001 cactatcaaa aacccttgaa tcatcatctt aaaagctgtg atggacagag catggaatcg
     6061 gtgaaatgaa cgctatgata tcgataacca tttatgaaaa tgaagggaag gaatgaacac
     6121 tacgtcgcaa gccagcggtg gagagactga aaaaccgtgg cagccttggt cccccggatt
     6181 tgcacaggag cgaccaccat ggcctgtcac actaaggtcc ttcgagggat cgattcttcc
     6241 ctggagagaa ggtgctttgg acattataag ttagagatgt ctccttatta ggaaatcact
     6301 agctatatat tgatattttt cgttataatt gttaattatt tttataggtc atattcttaa
     6361 ggttttttca tcctcaatat catacctttt ttacccagaa tggcgtatct tgatacattt
     6421 ttaccattag accgctcaat gttctttcat ccttaagtgt gtgcatgcga agaagtttgc
     6481 gaagctcgga tcttcgtcct ttcccccaag tgcagcgtct taactcattt tggaaagcct
     6541 tgtggacatc ggtttgattg tggactgaga gaatcaaaac gactctacag caatcgacga
     6601 cgtgcctcta aaataagggc atttagaacg aaagcaataa aaagttgttg ctccatttcc
     6661 tgaattatcg taactgtacc ctgtgtggcc ttgttgacta gatatgaagc agggggcaga
     6721 tatccgaaag actgagaagt tgcagagata taggtctgca ttttttagct aacgatgatc
     6781 agaaacgcat aagtaggaat acgcgatgta ttttaatttt ttcgggttac ggaccaaacc
     6841 atttaatacc act
//
LOCUS       DATIAL010000478         4950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1052943, whole genome shotgun sequence.
ACCESSION   DATIAL010000478 DATIAL010000000
VERSION     DATIAL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052943"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VLA60_14655"
     CDS             complement(<1..686)
                     /locus_tag="VLA60_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF10647.1"
                     /translation="MTTSPHIVIIGSGPGGYVAAIRAAQLGAMVTILEEQVLGGVCLN
                     WGCIPSKTLLGFIDLGERVRHAQPFGLNIEGPVSYDLARMVARKEEVVRGLVKGIGTL
                     LKQWKITHVPGRGELVNDRQVRVTHLDGSSSTIDADAIILATGSSWPQLPQFPIDGQR
                     VLTSKEALDLTEIPRSIVIVGGGVEGCEFSCLLSGLGSEVSIVEMMPSVLPLEDEDTI
                     STMTRELKKR"
     gene            complement(683..1069)
                     /gene="gcvH"
                     /locus_tag="VLA60_14660"
     CDS             complement(683..1069)
                     /gene="gcvH"
                     /locus_tag="VLA60_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HSF10648.1"
                     /translation="MEPQDLRFHKEHEWIRVEGKKATLGISNFAQDALGDVVFVDVPK
                     VGTSLQAEDQLGEVESTKATSTIYTPVTGKILQVNTELQDHPELLNQDPYGKGWIAVL
                     ELADPGEVDGLMTPEQYTAFLASQES"
     gene            complement(1184..2740)
                     /locus_tag="VLA60_14665"
     CDS             complement(1184..2740)
                     /locus_tag="VLA60_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10649.1"
                     /translation="MTTWLKICGLLVCLGLLAGCGRTRPPQGPPVSGAIPPPAVESVP
                     EDREEGIVPSPPEVPPDIQQIHAQLFQRSKEHFEEKAYPQAISGLTRLLALLPGEPIE
                     VQSRWLLAQAYRQVGEWEAARDQYRILTVAGSANPYQAGAQLQFNELQRLLEQFTMPP
                     LDTQAIRLTLEQLPASGGFDEGIKKMKADGITTLLIDLGCEGAGFHTDQQRTSRAPRH
                     LLDLQTLLRSYVERSHQLHLLVYVGVNLRCIGNWHDSPLPEWRDGTYNTITRQVQDSR
                     FFDIFHPEYQRFLDQFLTHLSEESVDGLVFLDDYPMGVYDGMSRAGLTRFQTAFGVNF
                     DPVRTFQSGFDPLRPSKLASRSTQPSGAPMEDSVFWRWAGWKARERLTVLEQLMGQLR
                     KRHLTVQCGLELHPHGLTDPVRALVDYAQDAMEAIRRPFSFFFVRPEIDRHSSSDQVS
                     AIEKLRRISTKAVLTRLLPVLDDPRRVWVSMPAERGKRIVPGTAGSSTSLLGEFPSGI
                     GVVHDLRAFS"
     gene            complement(2877..3293)
                     /gene="ndk"
                     /locus_tag="VLA60_14670"
     CDS             complement(2877..3293)
                     /gene="ndk"
                     /locus_tag="VLA60_14670"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HSF10650.1"
                     /translation="MSERTLAIIKPDAVAKHAIGDIIRRYEEAGLFPVAMKMMRLSKG
                     VAEGFYAVHRERPFFNDLTTYMSSGPVVVVVLEGKDAVKVHRDLMGATDPKKADPGTI
                     RAAHGASIEANAVHGSDSKENAQIEIRYFFAESNLG"
     gene            complement(3393..4688)
                     /locus_tag="VLA60_14675"
     CDS             complement(3393..4688)
                     /locus_tag="VLA60_14675"
                     /inference="COORDINATES: protein motif:HMM:NF026052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10651.1"
                     /translation="MHASHRVPLIYNLFPRLAGPCDRWISHAERAADMGFNWLFVNPI
                     HLPGFSGSLYAIKEYDLLNPAFLPEGTQDQRLDVLRPTLQRIAQCGLAPMVDLVLNHT
                     AIDSSLVSQHPDWYMRDGQGNVEHPYAVDPDDPSKKTVWGDLAEIDNRHSPDREHLWE
                     FWGQLVDRYLELGFQGFRCDAAYKVPAELWRFLIHRAQRVNPQAVFWAENLGCTLEQT
                     RALGEAGFHLFCNSSKWWNFKDAWCLSQHREFKAMPSIGFPESHDTPRLAAESGGNEA
                     VQRQRYAFAALFSTGLMIPMGYEFGFQRSLHVVETTPDDWESPRWDLQSFIRAVNQWK
                     LDVPLWQGEGDLVQRSMSNPNLCVLERRSLENPHSWALLCLNTHAHEPVDVAIGEVGP
                     LPSPPKMWRVSSLDQHPGSHSITERVVFQPAEVIVITAF"
     gene            complement(4699..>4950)
                     /locus_tag="VLA60_14680"
     CDS             complement(4699..>4950)
                     /locus_tag="VLA60_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF10652.1"
                     /translation="HVTFESQDGKFSACLTIKQAADFGILVYEQDGAPFPSERGGPFR
                     LVTPGLGDLCANVKQVGKIIFSKGLISDSRPPQACPEKA"
BASE COUNT         1257 a         1439 c         1202 g         1052 t
ORIGIN      
        1 ttgcgttttt tcaactcacg cgtcatggtg gagatcgtat cctcatcctc aagtggcagc
       61 acggacggca tcatttcgac aatactcact tccgatccca gtccgctcaa caagcaggaa
      121 aactcacatc cttccacccc accgccgaca atcacgatgc tcctgggaat ctccgttaaa
      181 tccagagcct ctttactggt caacacgcgt tgaccatcaa tgggaaactg aggcagttga
      241 ggccaggagg accctgtggc aagaataatg gcatcggcat caatggtaga agaggaccca
      301 tcaagatggg tgacccgcac ctgccggtca ttaaccaatt cgcctcgccc cggcacgtgg
      361 gtaattttcc actgcttcaa taaggtgccg attcccttta cgagcccacg aaccacttcc
      421 tccttacgcg caaccatgcg agcaaggtca tagctcacag gcccctcaat gttgaggcca
      481 aagggctggg catgccgaac ccgttcgcca agatcaataa agcccaacag ggttttactg
      541 ggaatacaac cccaattcag gcacaccccc cccagcacct gttcttccag gatcgtcacc
      601 atggccccta attgggcggc gcgaatagcc gccacatagc caccagggcc tgatccgata
      661 atcacgatgt gaggagacgt ggtcatgatt cctgtgaagc taaaaatgcc gtgtattgct
      721 ccggcgtcat caacccatcc acctcaccag gatcggctaa ttccagcacc gcgatccatc
      781 ccttgccata gggatcctga ttgagtagtt cgggatggtc ctgaagttct gtattcactt
      841 gaagaatttt tccggtaacc ggcgtgtaaa tcgtcgaagt ggctttggtc gactccactt
      901 ctcccaattg atcttcagct tgcagcgagg ttcctacttt aggaacgtcc acaaagacca
      961 catcccccag ggcatcctga gcaaaattgc taatccctaa ggtcgccttt ttcccttcaa
     1021 cgcgtatcca ttcgtgttcc ttgtgaaatc gtaaatcctg tggttccatt cctaccccct
     1081 tttgttcaaa acgatgatgg cgttcatatt tgtctgatgt ctcccaggcg cctcatgaag
     1141 tgagtgtgct cggctcaatg tgacagggac gctaccggaa cactcaagaa aatgcgcgaa
     1201 gatcgtgcac gacaccaatt cccgaaggga actcaccgag cagtgaggta ctgctcccgg
     1261 ctgttcccgg cacaatgcgc tttcctctct ctgccggcat actcacccat acccgccgcg
     1321 gatcgtcaag tacgggaagc aagcgggtta acacggcctt cgtcgaaatg cgccggagct
     1381 tctcaatcgc cgacacttga tcggaggagg agtgacggtc gatttccggc cgcacaaaga
     1441 aaaatgaaaa cgggcgccgg atggcttcca tagcatcctg ggcatagtcc accagagccc
     1501 gaaccggatc ggtcaatccg tgggggtgga gctccagacc acattgaacc gtcagatggc
     1561 gtttacgaag ctggcccatt aactgctcca gtacggtcaa tcgctcacga gccttccacc
     1621 ccgcccagcg ccaaaacacg gaatcttcca taggagcgcc tgaaggctgg gttgatcgac
     1681 tcgccaattt cgatggcctg aggggatcaa acccagactg aaaagtacgt acgggatcaa
     1741 aattcacacc gaaggcggtt tgaaaacggg tcaatcccgc ccgcgacatc ccgtcgtaca
     1801 cccccatggg ataatcgtcg agaaacacaa gaccgtccac gctttcctcg gaaagatgcg
     1861 tgagaaattg atcgagaaac cgttgatact ccgggtgaaa aatatcaaaa aaccgggaat
     1921 cctgaacctg gcgggtgatc gtattatacg tgccatcccg ccattccggc aaaggggaat
     1981 cgtgccaatt gccaatacaa cgcagattca ctcccacata caccaacaga tgcaactgat
     2041 gactgcgttc cacatatgag cgcaggagcg tctggagatc aagcagatgg cggggggccc
     2101 tcgaagtccg ctgttgatcg gtgtggaatc cggctccctc acaacccaaa tcaatcaata
     2161 gagtggtgat tccatcagcc ttcatttttt taatgccctc atcaaaccct ccgctagccg
     2221 gcaattgttc aagggttaac cggatggcct gagtgtcgag aggaggcatc gtgaattgtt
     2281 caagtaaacg ttgaagttca ttgaactgca attgagcacc ggcttgataa ggattggcgc
     2341 tccccgccac ggtgagtatg cggtactgat ccctggcggc ttcccattcc ccaacctgac
     2401 gataggcttg agcgagcaac catcggcttt ggacttcaat gggctcacca ggaagaagcg
     2461 ccagcaaacg cgtcagtccg ctgatggcct gcggataggc tttttcctca aaatgttcct
     2521 tggaccgttg aaagagttgc gcatgaatct gttgaatatc cggcggaact tcaggcgggg
     2581 atggcacgat tccttcctcg cgatcctccg gcacggattc gaccgcaggg ggaggaatgg
     2641 caccactcac cggtggtccc tggggaggac gtgtccgccc gcatccggcc aggagcccca
     2701 gacacaccag caaaccacat attttcaacc atgttgtcat acggcaatac gtgcgcagag
     2761 agaaatgaac cctcaaccca tacaccaggt ttatgaggag aaacatgccc gccgtttttg
     2821 gcgggcagcg tcggagccag tgggttttaa aaatacaggc tcagtatggt cttctactag
     2881 cccagattgg attctgcaaa aaaataccga atctcaattt gagcattctc cttggaatcg
     2941 gacccatgca ccgcattggc ctcaatagaa gccccatgcg cagcccgaat ggtacccgga
     3001 tcggcttttt tgggatcggt cgctcccatc agatcacggt gaactttgac cgcatccttc
     3061 ccttccaaca ccacaaccac caccggcccc gagctcatat aggtcgtcaa gtcattaaag
     3121 aaggggcgtt ctcgatgcac agcataaaac ccttccgcca cccctttgga taaccgcatc
     3181 attttcatag caaccggaaa caacccggcc tcttcatagc ggcggataat atcaccaatc
     3241 gcatgcttgg ccaccgcatc aggcttaata atcgctaacg tccgctctga catacttctc
     3301 gtcctttccc aaaaatccca taataaaacc ctgtgaataa gcagggagca ttttacggcc
     3361 ccttctattc agttgcaatc tcaaggccac acttaaaagg ctgtaatcac aatgacttcg
     3421 gccggctgga acacgacacg ctcagtgatg gaatgggaac caggatgctg gtcgagcgag
     3481 ctgacacgcc acatttttgg aggagacggt agagggccca cctcaccgat ggccacatca
     3541 accggctcat gagcatgggt gttgagacag aggagtgccc aactgtgagg attttccaac
     3601 gaccgccgct ccaatacgca caggttgggg ttggacatcg accgttgaac cagatccccc
     3661 tccccttgcc agagcggcac atccaacttc cattgattca ccgcacggat aaaactctgc
     3721 aaatcccatc ggggggattc ccaatcatcg ggagtggtct ccaccacatg cagagaccgc
     3781 tgaaatccaa attcataccc catcgggatc atgaggccgg tggaaaacag cgcggcaaag
     3841 gcatagcgtt ggcgttgcac cgcttcattc cctcccgact cagcagccaa acggggggta
     3901 tcatgcgatt caggaaaccc aatagacggc atagccttaa attcccgatg ctgactcaaa
     3961 caccaggcat ccttgaaatt ccaccatttg gagctgttac aaaaaaggtg aaatcccgct
     4021 tctcccagcg cgcgtgtctg ttccaaggta caaccaagat tttccgccca aaagacggcc
     4081 tgtggattca cgcgttgggc ccggtgaata agaaagcgcc acaactcagc tggaacttta
     4141 taggccgcgt cacaccgaaa cccctgaaac cctaattcaa gataccggtc aaccaattgc
     4201 ccccaaaact cccagagatg ctcccgatcc ggagaatgcc gattatcaat ttcggctaaa
     4261 tcaccccaca ccgttttctt gctgggatca tccggatcaa ccgcatacgg atgttcaacg
     4321 tttccctgac catcccgcat ataccagtcg gggtgctgtg acactaagga ggaatcgatg
     4381 gccgtgtggt tcaacaccag atccaccatt ggcgccaggc cacattgggc aatacgttga
     4441 agcgtgggac gtaacacatc aagtcgctgg tcctgcgtcc cttccggcag aaatgccgga
     4501 ttcaggagat catattcttt aatggcatat aaacttcccg agaatcccgg gagatgaatg
     4561 ggattcacaa agagccaatt gaaccccatg tctgcggccc gttccgcgtg cgagatccag
     4621 cgatcacaag gaccggccag gcgagggaac agattataga tgagtggaac gcggtgcgaa
     4681 gcgtgcatca agacaaacct acgctttctc cggacaggct tgaggtggac ggctatccga
     4741 aatgagacct tttgaaaaga tgattttgcc gacctgtttt acattggcgc acaagtcacc
     4801 caatcccgga gtgactaagc gaaatggccc gcctcgttcg gaaggaaacg gcgcgccatc
     4861 ctgttcatac accagaatgc caaagtctgc ggcttgcttg atggttaaac aggccgagaa
     4921 ttttccatcc tgcgactcga aggtcacatg
//
LOCUS       DATIAL010000479         3986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1052946, whole genome shotgun sequence.
ACCESSION   DATIAL010000479 DATIAL010000000
VERSION     DATIAL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052946"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VLA60_14685"
     CDS             complement(<1..441)
                     /locus_tag="VLA60_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10653.1"
                     /translation="MAKAFSVASWNVEHFGAMTKKKAKPKKPVGPIIEFLARQHADVV
                     AVYEVLGSTVYDTIVTVMPDYQFHITEGPQTQEILIGVKRTFSSFFTQRLEFNSGVSV
                     LRPGALLTVQVEGKAYPLLFLHLKSLTEPRGFGLRDDQTERALKL"
     gene            complement(640..1119)
                     /locus_tag="VLA60_14690"
     CDS             complement(640..1119)
                     /locus_tag="VLA60_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10654.1"
                     /translation="MPAYVVTWIHLLAVITFIGGWMFVQLVVRPSLTEGKAKVPSMEA
                     LEMVKKVGQRFKMVGWVSLMALVFTGASQLLDESGSARIETSWGLIMMLKLFVFAIVG
                     GLIFVHDLILDPYGVSGKSKKTETEPLLFSGKVVWVQRAILSLSLAVLFIAAYLTSL"
     gene            1265..1951
                     /locus_tag="VLA60_14695"
     CDS             1265..1951
                     /locus_tag="VLA60_14695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF10655.1"
                     /translation="MMKIRFQLFPNLSQIFVLGLLGPFALAGFMGIDSSWGAEADKER
                     WNKKYETENYLFGRDPIPFLRNHVDLLPQGSALDLAMGEGRNGVFLATKGFQVTGVDI
                     SEAGFQKARALAAEKGVKLTTVVADLEQYTIPPNSYDVIICTYFLQRDLFPKITAALK
                     PGGVVLIETYTVDHLQYRPQFNRTFLLERNELLTLLPGLRVLRYQEVDTGDAAFASIL
                     AQKPLQATQQ"
     gene            2083..3600
                     /locus_tag="VLA60_14700"
     CDS             2083..3600
                     /locus_tag="VLA60_14700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF10656.1"
                     /translation="MTQTHASQPTADTWAGVTPQVLEGDILNQRAWRMPDIVIYQHAP
                     DEWWVAPLDEELPLIRLNRLGAALLGAMDGRMTIGALLNQYGKWVCSPTQQNGRWHLE
                     RWAQPRFSLAYFGTEPPSGHSAEAKWDLLLQKVREGWHQNVQAETEDHLSKFHVQGIQ
                     GPHGHFEIIETTVSHLFREPCEAMNGLTYGRLLGKTLRQMGWLSPKPKRIVEVGAGLG
                     YVSKELAGELSAEERKEIQYTFLDLTGPFLGSQTSLARQAGWTATAIQANAERMPLAD
                     RSVDLLIDNENLADMTPIQFAGDELLTFRGENPLHEEALDLIRRMRLPLMPPFPDDVI
                     FNYGAIQFLQEVWRVLKPGGRAILVEFGIEDDWPSPVRLPGHTEYEVQFSHLRHAAKW
                     LGFREQYCTLPQLLGMKRDINVLCTGAAYTLRRFCRELEKPFSVRAYTEKELGTALGA
                     LLPKLTGLHYHNVLDPAWFGLWDFKVLLVEKPGGMSIGQQPAFKESGGFRWYSQR"
     gene            complement(3619..>3986)
                     /locus_tag="VLA60_14705"
     CDS             complement(3619..>3986)
                     /locus_tag="VLA60_14705"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HSF10657.1"
                     /translation="AKVSKVFGPFSGIPLVGTAGSVTTLAAMAQGLPRYESAHVHNYE
                     LTLSIIKGLEQDLITKTGPQRLAMPGLEPGREYVIVAGTVILRRIMEIFGFDACLVSD
                     FGLREGILVDKARKLKNVE"
BASE COUNT          983 a         1100 c          967 g          936 t
ORIGIN      
        1 caatttgaga gcccgttcgg tctgatcgtc ccgcaaccca aaccctcgag gttccgtcag
       61 actttttaaa tgcaaaaaca aaaggggata ggctttcccc tccacttgaa ccgtgagcaa
      121 ggcacccggg cgcagaacgg atacaccaga attaaattcc agccgttggg taaaaaagct
      181 ggagaaggtt cgcttcacac ctatcaaaat ttcctgcgtt tgaggcccct ccgtaatatg
      241 gaactggtaa tccggcatca ccgtcacaat ggtgtcatat accgtcgagc ccaagacttc
      301 gtacaccgcg accacatcgg catgttgtcg ggccagaaac tcgataatgg ggccgaccgg
      361 ttttttcggc ttcgcctttt ttttggtcat cgccccgaaa tgctccacat tccaagaggc
      421 aacggagaat gctttggcca tctcctcctc cttgatgacg tgtgataggt cactaaatct
      481 ttaccttttc caaggttgta ccataggctt ggaaaaagaa aaagatcgct taaggggcct
      541 aaaaccctct cacgatccct tcggtttata aggttgggtt tttcagcaga gaggcttttt
      601 ggcagaaaac agttggcaac aatgggaaaa tttctcgcgt caaagcgacg ttaaataggc
      661 cgcaatgaac aagacggcca ggctcagact caagatggct cgttggaccc acactacttt
      721 gccggaaaac aggagtggct ccgtctcagt ctttttgctt tttccggaga ctccataggg
      781 atcaagaata agatcgtgga cgaaaatcaa tcctccaaca atcgcaaaga caaaaagctt
      841 gagcatcatg atcaagcccc aactggtttc aatacgtgct gatccgcttt catccaataa
      901 ctgagacgct ccggtaaaga ccagcgccat gagactcacc caccccacca ttttaaaccg
      961 ttggccaact tttttgacca tctcaagagc ttccatcgac ggtaccttgg ccttgccttc
     1021 tgtcaaactt ggcctgacga cgagctgaac aaacatccat ccgccaatga aggtgatgac
     1081 ggccaataaa tgaatccacg tgacaacata ggcaggcata tgttttctcc tacaatttcc
     1141 attcatctcc caaacggtgc cataataccc actgaaacag ggaaagacca ccaagaaacg
     1201 tgctgaattc tttcgtcccc cgactgaaat cggctatgat tctctcaaca gctgtggaga
     1261 tcaaatgatg aaaattcgct ttcaactatt tccaaatctt tcccaaatat ttgtcctggg
     1321 gcttctgggt ccattcgccc ttgctggatt catgggcatt gactcctcgt ggggagctga
     1381 agcagataag gagcgatgga ataagaaata tgaaaccgag aactatctat tcggacgtga
     1441 ccccatcccg tttttaagga accatgtcga tcttctaccc caggggtctg cccttgacct
     1501 ggccatgggt gaaggacgca acggggtctt tttagccaca aaaggctttc aggtcaccgg
     1561 ggtcgatatt tcagaggcgg gtttccaaaa agcccgagcc ctcgctgccg agaaaggcgt
     1621 gaaactgacc accgtcgtcg ccgacctgga acaatacacg attcccccca attcctacga
     1681 tgtgattatc tgcacctatt tcctgcagcg cgatctcttc ccaaaaatca ctgctgccct
     1741 caagcccggc ggggtggtcc tgatcgaaac ctatacggtt gatcatttgc aataccgccc
     1801 acaatttaac aggaccttcc tcctggagcg aaatgaatta ttaaccttgc tccctggcct
     1861 gcgggtattg cgttatcaag aagtcgatac tggagatgca gcctttgcca gtatcctggc
     1921 acaaaaaccc cttcaggcca cacagcaata agtgccagga ctttggtttc tctccctcct
     1981 cctgacaagg aaagaaatgg ttgcggcctt ttggatcgat ggggtaccct ggcagcttcc
     2041 tttttgtccg tgcatgctga ccagaatcgg aacccaaccc tcatgaccca aacccatgcc
     2101 tctcaaccca ctgccgatac atgggcagga gtcacccctc aggtcttgga gggggacatt
     2161 ctcaatcaac gggcatggcg catgcccgat attgtgattt accagcatgc gccggatgaa
     2221 tggtgggtgg ccccacttga tgaagaatta cccctgattc ggcttaatcg cttgggcgca
     2281 gccctgctcg gcgcgatgga tggtcgtatg accattggcg ccctactcaa tcagtatggg
     2341 aaatgggtct gcagcccgac ccaacagaat ggtcgctggc atttagaacg gtgggcccaa
     2401 ccgcggtttt cgttagccta ttttggaacc gaacccccca gcggacacag cgcagaggcg
     2461 aaatgggatt tgttattgca aaaagtgcgg gaagggtggc atcaaaacgt ccaggctgaa
     2521 accgaagatc atctgtccaa atttcacgtt cagggcattc aagggcccca cgggcatttt
     2581 gaaatcattg aaacgacggt ctctcattta tttcgcgaac cctgcgaagc catgaacggg
     2641 ctgacctatg ggcgactgct cggcaaaacc ctgcggcaaa tgggctggct ctcccccaaa
     2701 cccaaacgca ttgtggaagt gggcgcgggc ctcgggtatg tctcaaagga actggcagga
     2761 gaactctcag cggaggagcg gaaagagatc cagtacacct ttctggattt gacaggcccg
     2821 tttctgggat cccaaacgtc gctggcgcgc caagcagggt ggacggcaac ggctattcaa
     2881 gccaatgccg aacgaatgcc actggcggac cgttccgttg atttattgat cgacaatgaa
     2941 aatcttgccg acatgacgcc aatccaattt gccggtgacg aactcctcac gtttcgaggc
     3001 gagaatcccc tgcatgagga agccctggac cttatccggc gcatgcgcct gccactcatg
     3061 ccaccctttc ccgacgatgt gattttcaat tacggcgcca ttcaattctt acaggaagtc
     3121 tggcgcgtgc tgaaaccggg cggacgtgcc atcctggtcg aattcgggat tgaagatgat
     3181 tggccttctc ccgtcagatt gcctgggcac actgaatatg aagtgcaatt cagtcatctc
     3241 cgtcatgccg ccaaatggct gggtttccgc gagcagtatt gcaccctgcc gcaattactt
     3301 ggcatgaaac gggacattaa tgtcttatgt accggggcgg cctataccct acggcgattt
     3361 tgtcgtgaat tggaaaagcc cttctccgtt cgggcctata ccgaaaaaga attgggcacg
     3421 gcgctgggcg ctcttctacc caaactcacc ggcctgcatt accataacgt cttggatccg
     3481 gcatggtttg gtctatggga tttcaaagtc ttgttggtcg aaaagcccgg tggcatgagc
     3541 atcggacaac aacccgcctt caaagaatcc ggcggtttcc gctggtacag tcagcgatag
     3601 gacccatccc tacccttctt attccacgtt ttttaatttt ctggctttat caaccaggat
     3661 tccttcgcga aggccaaagt cgctgaccag gcatgcgtca aaaccgaaga tttccatgat
     3721 tcttcgtaga atcaccgtgc ctgccacgat gacatattcc cgcccgggtt caaggcctgg
     3781 catggccaag cgctgcggac cggttttggt gatgagatcc tgctccagcc ctttgatgat
     3841 cgacaacgtt aactcataat tgtggacatg tgccgactca tatctcggca gtccttgagc
     3901 catcgccgcg agtgtggtga cggaacctgc cgtcccgacc agcgggatgc ctgaaaaagg
     3961 cccgaaaact ttcgatacct tggcca
//
LOCUS       DATIAL010000480         9927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1268846, whole genome shotgun sequence.
ACCESSION   DATIAL010000480 DATIAL010000000
VERSION     DATIAL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268846"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1128)
                     /locus_tag="VLA60_14710"
     CDS             complement(184..1128)
                     /locus_tag="VLA60_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF10658.1"
                     /translation="MRTTVALTGASGFIGGVIAQHLRKGGWHVRGLARSPRQAQQLED
                     LGVTPVQGQLEDPASLLTLVKDSVSVIHCAGAIRGVSREDFYPTNVMGVSNLIQACLA
                     QSSLPRFVLLSSLAARKPSLSPYAWSKHEGESVLIREAGLLKWSIFRPPAVYGPKDRA
                     LLPLFTLLRKGIGVQLGPSHAKFSLIHVEDLANAILHWLKSGDPPFQTFEIDDGFAGG
                     YSWEDIFRLSNPRLRLWLRIPPSLLQIGGRSNEICSRLFGYTPLFTRGKVAELLHPNW
                     VCNSSHATQTLGWTPQISLKEGLRRLFTSDRLMPFHRN"
     gene            complement(1165..2931)
                     /locus_tag="VLA60_14715"
     CDS             complement(1165..2931)
                     /locus_tag="VLA60_14715"
                     /inference="COORDINATES: protein motif:HMM:NF006624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HSF10659.1"
                     /translation="MNATPTHHQLRLKHGNFSTLSEALDYAASGETGCNFYSGRGKLT
                     HVLTYADLKQQAQNLARRLNGLELSRGARVALIADTTPDFIRFFFACQYAGLIPVPLP
                     ISLHLGGHQAYVTQLRRLLDSCQASIAMAPEGFLPFLEEAATGLNLVLIGGPETFASL
                     EEIQGPLSPLEPQELAYIQYTSGSTRWPRGVEVTQAAVMTNLSGIIQHGLQVQPGDRC
                     VSWLPFYHDMGLVGFVLGPIASQLSVDYLSTRDFAMRPRQWLDLMTKSRATISFSPSF
                     GYELCVRRLGEDFNEHYDLSSWRIAGAGAETIRADSLSRFAKTLKSSGFKPEAFLACY
                     GMAECSLAISFAPLNKGLEVDRVDANELSNNRLAMPFVPFSTDEEQARCSKFVNCGVP
                     LPGYEVEIRNMTGEVVKERESGIIFVRGASTMTGYFNDPILTQEVLSTDGWLNTGDIG
                     YWVENSLVIIGRSKDMIIINGRNIWPQDLEFMAEQQAEVRPGDSSAFSVSTPDGHEIA
                     IMVVQCREMDNKKREDLVQRLRSLVHKELSIPCYIELIPPRTLPRTSSGKLSRSRAKA
                     DFLTRIQWEEVTTHLSTTARSA"
     gene            complement(3149..3592)
                     /locus_tag="VLA60_14720"
     CDS             complement(3149..3592)
                     /locus_tag="VLA60_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HSF10660.1"
                     /translation="MNTTMKKFGYPNACLKEFSQWVVLLRPQQVTLGSLVLICREEAT
                     AFSQISAQAFTELPKIIREIETSVSRTFSYSKINYLMLMMVDPEVHFHVIPRYDTPRL
                     FSQQQFLDHGWPGPPALKNANDISEEVYQNLLSHLKNNWIHEFAN"
     gene            3982..4716
                     /locus_tag="VLA60_14725"
     CDS             3982..4716
                     /locus_tag="VLA60_14725"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HSF10661.1"
                     /translation="MLTSQWYREKYLLDRMTFRDLRKAYVLYPSIQIYAVLLACSIIG
                     AFVVFPYFPVSPLRVAGSIVGTLLAYPFVEYAVHRFILHGRYLYRSPWTAALWKRIHY
                     DHHQDPNDLRVLFGAGSTTLPTIVVATFPIGMSLGGWVGAALSFATGLACFMVYEFCH
                     CMQHLRVSPKNRFLRRVKRHHLLHHFHNEKGNFGITSLFCDRIFGTEYGNAEDVPFSQ
                     TVSNLGYADQERSHYPWVAQLSEQKP"
     gene            4789..5940
                     /locus_tag="VLA60_14730"
     CDS             4789..5940
                     /locus_tag="VLA60_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10662.1"
                     /translation="MPRWVGKGVLIRVEPVGDRAAFKEFIRLPWKLYRSDPHWVPPLW
                     IERLHTFSPKNPYFHHARWQCWLAYRGQEPVGRICAQIDDLHLSKYQDQTGFFGLLEA
                     EEEASVFQALFQTAETWLQTHGMTNVRGPFNLSINQECGLLVDGFDSPPMIMMGHARP
                     YYAQLVERNAYEKAQDLLAYRIGIHFSFPSGVSLFLKKAKTSMRIRPLRRKHLKEDLA
                     IIKDIFEEAWSDNWGFLPFTDAEFAEIGQQLKLLVEDDFVQIAEIDGEPTAMLVAFPN
                     IHEVIRDLNGQVFPWGWLKILWRLKVSYPQSARVALMGVRKRFQSSPMGAAMAYGLIE
                     AVREAGLRKNIQQIETSWILEGNKRMRHILESLGAEPYKTYRIYEKHLQ"
     gene            5968..6777
                     /locus_tag="VLA60_14735"
     CDS             5968..6777
                     /locus_tag="VLA60_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HSF10663.1"
                     /translation="MGLDYQSVTALVLAGARTGRDPVARMAGVANKVLAQVGGEPMIS
                     RVLETLAQSDGVGKRILCGPSWETVQDNSFLGTLIASGAVQWVEPAQGPSLSVARFLN
                     EHPQEFPVLVTTADHALLTPEMVNYFLREAQEKQMDVAVGLVPYSLVVASYPQSKRTV
                     TRLTGGGFCGCNLFVLCTPKAKRMVEFWSRIERERKHPLRLIRTLGGLALVRYVLGWL
                     SLAEALRRLGQKLNLHVQEVLLPFPEAAIDVDTPEDLALAEEILAKRQATF"
     gene            complement(6877..7596)
                     /locus_tag="VLA60_14740"
     CDS             complement(6877..7596)
                     /locus_tag="VLA60_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HSF10664.1"
                     /translation="MKAVILSAGQGKRLLPHTIDKPKCTVPVNGLPIVKRQIQALLRA
                     GIDSIYIVVGFGAEKVETLLTDEIASGSVRTIYNPFFAMADNLMSCWAARNDMSQDFL
                     LINGDTLFESAVLDGLLQAPSSPITLVTDEKNRYDADDMKVIVRGSQLDRIGKTLSIE
                     QVNGESIGMIRFQGNGPKIFLSTLDQRIRKPEALKQWYLSLIDELAQQGHVSTFCIKG
                     LKWAEIDNPSDLEQAERLFKN"
     gene            7656..8714
                     /gene="lptF"
                     /locus_tag="VLA60_14745"
     CDS             7656..8714
                     /gene="lptF"
                     /locus_tag="VLA60_14745"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HSF10665.1"
                     /translation="MLIKYLIREVLKPSAVVCGLLVALFAGYSWVTFLAKAVNALLPV
                     NMILTLIALKVGIALEVLIPISLYFGVILGFGRLYTDSEMKAFMACGVNPYRILLTVF
                     SLSCLVAVLVSIFSLYLRPLAYEEIYRLRDEGEAGFRLSNLDAGHFYERRNGSLVFFA
                     EDIDERHQKLSEVFVQSEHGETLQVFSAKTAQEQLDPQSGISIPVLFDGFEYKLTREG
                     EIKHISSFSRMAIYPQELVAEYRRKAESTQNLLKSDSRKDITELQWRFLAPFSAILMG
                     LLGVPLSRASPRQGKYAKIFTATVIFSVYYFTGLMVRNLVEQGILPIIPGLWWIVLVL
                     GAILAYWLAPSRFTRVTS"
     gene            8810..9880
                     /gene="lptG"
                     /locus_tag="VLA60_14750"
     CDS             8810..9880
                     /gene="lptG"
                     /locus_tag="VLA60_14750"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HSF10666.1"
                     /translation="MKILNRYLAVSLLKGYFPVLAIFLAVFSLIVFVEELDDVGKGRY
                     TFWSAGEFLLLTLPNRLVFLAPFVSLLGSIIALGSLANGRELIAIQAAGVSPYQIAWS
                     VMKVGACFILLVGFLEEVVNPPLDQEAYFKRSMALSDSGAYKGKQGFWFRDGRRFIRI
                     HQIRYGESPENIDIFEFNDAGQLDFYMHAQEAEIVNPQKWTLKNIKKQVIHGYSVSQE
                     QMESLEWDSPLKQNEVRLLTLPSSTLAPSELYQYMEVLKRKKQNYLRYELSFWDKIFM
                     PLNTGLMILVAVPFVFGPLRMSAAGKRVFIGSLVGLGYYLMTEISKHVGILFGASPLL
                     TTAAPFLLLSLVTFVLWRRYLN"
BASE COUNT         2450 a         2149 c         2566 g         2762 t
ORIGIN      
        1 ttaaaaggac ttgcatgact tgtaaggaat ccaatcccag ctctgcgacc agctcagttg
       61 attcttttaa ccgaaagccc tgtttggtaa aaggcttgag agtgtcgaac agttggggaa
      121 ggatgtcgga ataactcatc atatataata gccttcttga tggatgaaga gaacaaaccg
      181 gtactaattc ctatggaatg gcatgagcct atccgatgtg aacaaccgac gcaagccttc
      241 tttgaggctg atttgtggcg tccagccaag ggtctgcgtc gcgtgagaac tattacagac
      301 ccaatttggg tggaggagtt cagcaacctt cccgcgggtg aaaagaggag tatacccaaa
      361 aaggcgggaa catatttcat tacttctccc tcctatttgt aacaggcttg gtggaatgcg
      421 caaccacagg cggaggcgag ggttggaaag acgaaaaata tcctcccagg aatatcctcc
      481 agcgaagcca tcatcaatct caaatgtctg aaagggtggg tctccgcttt ttagccaatg
      541 aagaatagcg ttggcgagat cttcgacatg aattaaggag aatttggcgt gggagggccc
      601 gagttgaacc ccaatccctt tgcgtaacaa agtaaagagg gggagcagtg cacggtcctt
      661 cggtccatag acggcaggtg gacgaaaaat actccacttc agtaaccccg cctcacggat
      721 taaaacagat tcaccttcat gtttactcca tgcatatggt gagagtgatg gcttacgagc
      781 ggcaagggat gagagaagaa caaaccgtgg aagagaggac tgtgcaagac aggcttgaat
      841 caggttagag actcccatta cgttggtcgg atagaagtct tctcgactca caccgcgaat
      901 cgctcctgcg cagtgaatga cactaaccga gtctttgact agggttaaga ggctggcagg
      961 atcttccaat tggccctgga ccggtgtgac tcccaggtcc tcgagctgtt gagcctggcg
     1021 gggggagcgc gcgagtccac gcacatgcca tccgcctttc cttaaatgtt gtgcgatgac
     1081 tcccccaatg aaaccggaag ccccggttag ggcaacggtg gtgcgcatag accgtggagg
     1141 acgtgtatgg aaaggtgcct ggtcttaggc agacctagcg gtggtagaca agtgtgtggt
     1201 cacctcttcc cactgaatgc gggtgaggaa gtccgctttg gctcgagatc gagatagttt
     1261 tcctgatgac gtccgtggaa gtgtccgcgg tgggatgagt tcaatgtagc atggaataga
     1321 caactctttg tggactaagg atcgtagccg ctgaaccaag tcttctcgtt ttttgttatc
     1381 catttcacga cattgcacca ccatgatcgc gatctcatgg ccgtctggtg tggaaacaga
     1441 aaaagcagat gaatcaccag gtcggacttc cgcctgttgc tctgccatga attctaggtc
     1501 ctgaggccag atatttcgtc cattaatgat tatcatatct ttggaccgac caataatgac
     1561 gaggctgttt tcgacccaat agccgatgtc gcctgtattg agccagccat cagtggagag
     1621 aacttcctgg gtcaagatgg gatcattgaa gtatcccgtc atggtgcttg ccccacgaac
     1681 aaaaataatt ccactttctc gttctttaac cacctctcca gtcatatttc gaatttcgac
     1741 ttcatatccg gggagaggaa ctccgcaatt tacaaatttg ctgcatcgag cctgctcctc
     1801 atcagtcgag aatggaacaa agggcatggc tagcctgtta ttggaaagct catttgcgtc
     1861 aactctgtct acctcgaggc ctttgttcaa gggagcaaag ctaattgcca gagagcattc
     1921 agccatccca tagcaggcca gaaatgcttc aggtttaaac ccgctggact tcagagtttt
     1981 agcaaaacgt gagaggctat cggcacgaat tgtttctgct ccagcaccgg caattcgcca
     2041 agagctgaga tcatagtgtt cattaaagtc ttcgcccagt cttcgcacgc agagctcgta
     2101 tccaaaagag gggctgaatg aaattgtcgc ccgactcttc gtcattaaat ccagccactg
     2161 acggggtcgc atggcaaaat cgcgtgtgct cagataatca acggaaagct gtgaagcgat
     2221 tggaccaaga acgaaaccga ccagtcccat atcatggtag aagggaagcc aggagacgca
     2281 acgatcgcca ggttgaactt gtaagccatg ctggatgatt ccagagagat tggtcatcac
     2341 ggccgcttga gtgacctcga cacctctagg ccaacgggtg ctgccggagg tgtattgaat
     2401 ataggctaat tcttgaggct caagtgggct caggggacct tgaatttctt ctagcgaggc
     2461 aaatgtttca ggacctccaa tgaggaccag gttcaacccg gttgctgctt cctccaaaaa
     2521 tggcaagaag ccttcaggtg ccatggcaat ggaggcttgg cagctatcga gcagccgtct
     2581 aagttgggtg acgtatgcct gatgcccccc taagtgcaat gaaatcggga gaggaaccgg
     2641 aatcagtccc gcatattgac aggcaaaaaa gaatcgaatg aaatctggtg tggtatcagc
     2701 aatgagagct acccgagccc ctcgtgataa ttccaatccg tttaatcttc tagctagatt
     2761 ttgcgcctgt tgtttcaggt cggcatatgt caggacgtgg gtgagtttgc ccctccctga
     2821 ataaaaatta cagccggttt ctcctgaagc cgcataatca agagcctctg aaagggtgga
     2881 gaagtttcca tgtttcagtc tgagttggtg atgagttggt gtagcgttca ttctatctca
     2941 aggattaaag gcagcattat tcccagtggt ttgctaaaaa gcatttaatt aagtaagcaa
     3001 ttgtaatgcc attaaatttt gtcggttaat tgccaagaac gaaggaaaag tgaaaacaaa
     3061 tttttctgtt cacattaagt tgtagcaaaa aaaccgcaaa ttgagtctat tttgcaacat
     3121 tacatttcat gtttttaagt atttacaact agtttgcaaa ctcgtgaatc cagttatttt
     3181 tgaggtgact taaaaggttt tggtatacct cttccgatat gtcattcgca tttttaaggg
     3241 ctggtggccc aggccatcca tggtcaagga attgttgttg agaaaataat cgaggtgtgt
     3301 catatcgggg gatgacatga aaatgaacct ctgggtcaac catcatcaac atgaggtaat
     3361 ttattttgct atagctaaat gtgcgtgaga cacttgtctc aatctcacgt atgatctttg
     3421 gcagttctgt aaatgcctgt gcactaattt gggaaaatgc ggtagcttct tcacgacaaa
     3481 ttaataccag tgaaccaagg gttacttgtt gggggcgtaa aagcacaacc cattgggaaa
     3541 attctttgag gcaggcgtta ggatatccaa attttttcat cgtggtgttc atgagcacgc
     3601 ccttataccc aaagagtaat ccttgtgtct atgaggcaat ggagccccct tgttcccata
     3661 ggcctatgtt gtcattctgc gcggcacgtt gaaatatagc acagcttttg tgtcagaacc
     3721 ggatttcaac attatcatgt ggcaaggtta ggtattgagc caaaggggag cgagtgaggt
     3781 gattgtgtgt tggtgagaga acttatggct ttcgtccatg atctctgcag gcttccaatt
     3841 ggaggtatcc tttcaggatt gagtattctt gtggcttctt ctgaaggttt ctataatcgg
     3901 tgcgtgagta attgcctggc gattcaggtg atagtgggtt gatggcagtg gacacatgca
     3961 aaaagggatg gaggctctag ggtgttaacc agtcaatggt atcgtgaaaa gtatctcctt
     4021 gatcgcatga catttcgcga tctaaggaag gcgtacgttc tatacccgag tattcaaata
     4081 tatgccgtgt tattagcttg tagtattatc ggggccttcg tggttttccc ttatttccct
     4141 gtttctccat tgcgtgtggc aggaagtatc gtggggactc tgctggctta cccattcgtg
     4201 gaatatgctg tacatcggtt tattttacat gggcgatatc tctatcggtc gccatggacg
     4261 gcagctttgt ggaaacggat tcactatgat catcaccaag atcccaatga tttgcgtgtg
     4321 ttgttcggag caggttcaac taccctgccg acgattgttg tggccacctt ccccattgga
     4381 atgagtcttg ggggctgggt aggggctgct ctttcttttg cgacaggatt ggcgtgtttt
     4441 atggtgtatg aattctgcca ttgcatgcag catttacgtg tgagtcctaa gaatagattt
     4501 ttacggcggg tcaagcgaca ccatttactg catcattttc ataatgaaaa aggaaacttt
     4561 ggcatcacga gtctgttctg tgatcggata tttggaactg agtatgggaa tgccgaagac
     4621 gtgccgttca gtcagactgt ttccaacctg ggctatgcgg atcaggaacg atctcattat
     4681 ccctgggtgg cccaactatc cgaacaaaag ccataagaga ctcatggcca ttcagaacag
     4741 tgatcctttt tccgcctcac aaatcctgac tccctggagt cgagtggtgt gccaagatgg
     4801 gtggggaaag gcgttttgat tcgtgttgaa ccggtcggcg accgcgccgc cttcaaagaa
     4861 tttattcgtc ttccctggaa gttgtaccgc tcagatcctc attgggttcc acctctatgg
     4921 attgagcgac ttcatacttt ttctccgaaa aatccgtatt ttcaccatgc tcgttggcaa
     4981 tgttggctag catatcgcgg gcaggaacct gttgggcgaa tttgtgccca gatcgacgac
     5041 ctgcatctca gcaagtatca ggatcagacg gggttttttg gcttgcttga agcagaggag
     5101 gaggccagtg tctttcaggc gctgttccaa acggcggaaa cctggcttca gactcatggg
     5161 atgacgaatg ttcgggggcc gtttaatttg tccatcaatc aggaatgtgg tttgttggtc
     5221 gatggatttg attcaccgcc catgattatg atgggccatg ccagacctta ctacgcgcaa
     5281 ttggttgaac ggaatgccta tgaaaaggcc caagatcttc tagcctatcg gattggaata
     5341 cattttagtt ttccctccgg agtttctcta tttctcaaaa aagcgaaaac ctcgatgcgt
     5401 attcgtccgt tacggcggaa gcatctaaaa gaggatttgg cgattatcaa ggatattttc
     5461 gaagaggcct ggtcagacaa ttgggggttt cttccattta ccgatgccga atttgccgag
     5521 atcggtcagc agcttaagct tttagtcgaa gatgattttg tgcagatcgc tgaaatcgat
     5581 ggagagccca cggccatgtt ggttgccttt cccaatatac atgaggtcat tcgggatctg
     5641 aacgggcagg tgtttccatg ggggtggcta aaaatcttat ggcggttgaa agtctcctat
     5701 ccccaatcgg ctcgggttgc cttaatggga gtgcgtaaac gattccaatc cagtcccatg
     5761 ggggcggcga tggcctatgg gttgattgaa gctgtgaggg aagctggtct tcgtaaaaat
     5821 atccagcaga tcgaaacttc ctggattttg gaggggaata aacgcatgcg tcacatcctg
     5881 gaatcactcg gagccgaacc atacaaaacc tatagaattt atgagaagca tcttcaatga
     5941 ttgccgatat ctgtttcgtg aggacatatg gggcttgact accaatctgt cacggctctg
     6001 gtattagctg gtgcccgaac cggtcgagac ccagtggccc gaatggcagg ggtagcaaat
     6061 aaggtcctgg cacaggttgg tggggaacct atgatctccc gggtattaga gaccttagcc
     6121 caatcggacg gagtgggaaa aagaatttta tgcgggcctt cctgggaaac ggtgcaagat
     6181 aattcatttt tagggacatt gattgcgtca ggagcggtcc agtgggtgga acctgcacaa
     6241 ggtccgagtc tcagcgttgc gaggtttcta aatgaacatc ctcaggaatt ccctgtcctg
     6301 gtcacgacgg cggaccatgc cctgttaaca ccagaaatgg ttaattattt tctgcgggaa
     6361 gcccaggaaa aacagatgga tgtggctgta ggtctggtgc cgtattcctt ggttgtggcg
     6421 tcctaccctc aatcaaagcg aacagtcact cgactgacgg gcggagggtt ctgtggatgc
     6481 aatttatttg tcctctgtac accaaaggct aagcgaatgg tggagttttg gagccggatt
     6541 gagcgtgagc gcaagcatcc ccttcgcctg attcgaacgc ttggggggct ggccctagtc
     6601 cgctatgtgc ttggttggtt atcccttgcg gaagccctga ggcggctggg gcaaaagctc
     6661 aatcttcatg tgcaagaagt gctgcttccg tttcctgaag cggccatcga tgtggatact
     6721 cctgaagatc ttgccttagc ggaagaaata ttggcgaaac gacaagcaac cttctaggag
     6781 ggaatggcct tatggtactt ctgcaggttt tggcagaccc gaattttgca acaatcaact
     6841 agggtgagca atcaacgaaa aagcttttcg tgattgctaa tttttgaaca gacgctccgc
     6901 ttgttctaaa tcagaaggat tgtcgatctc agcccacttc aaccctttga tgcaaaaggt
     6961 cgacacatga ccctgttggg ctaattcgtc gatgagtgat aagtaccatt gcttgagcgc
     7021 ctccggtttg cgaattcgtt ggtctaacgt ggaaagaaag attttaggtc cattgccctg
     7081 aaatcgtatc atgccgatgg actctccgtt cacctgttcg atggacaggg ttttgccaat
     7141 tcgatccaat tgtgagccac gcacaatgac tttcatgtcg tcagcatcgt agcgattttt
     7201 ctcatcggtc actaaggtga tgggggaaga gggagcctgc aacagaccat caagaacggc
     7261 tgattcaaat agagtatctc catttattaa tagaaaatcc tggctcatgt cgttgcgagc
     7321 cgcccaacag ctcatcaaat tatctgccat ggcaaaaaat gggttataga tggtgcggac
     7381 agagccagag gcaatttcgt cggtcagaag agtttcaacc ttttccgccc caaacccgac
     7441 cacaatgtaa atggaatcga ttcccgcgcg aaggagtgct tggatttgcc gtttcacaat
     7501 gggcagtcca ttgacaggaa cagtacattt gggtttatcg attgtgtgcg ggagtaaccg
     7561 ttttccctgt ccagcactta atattacggc tttcatcgtt gcaagtctcc tagtatgaga
     7621 tattccatgt gtactgtcgg cctgagggtc atttgatgtt aatcaaatat ttaattcgtg
     7681 aagtcctgaa gccatcagcc gtcgtctgtg gacttttggt ggccttgttt gccggatata
     7741 gttgggtcac atttttagcc aaagccgtca atgccctgtt gccggtaaat atgattctca
     7801 ccttaattgc gttaaaagta ggaatcgcgc ttgaagtcct tattcccatt tcgttgtatt
     7861 tcggcgttat tttggggttt ggccgattat ataccgactc tgaaatgaaa gcctttatgg
     7921 cgtgtggggt taatccatac cggatcctcc ttacggtttt ttccctttcc tgtttggtgg
     7981 ctgtccttgt cagcattttt tctttgtatt tgcgtccatt ggcctatgaa gaaatctatc
     8041 gattaagaga tgaaggagag gctgggtttc gtttgagcaa tttggatgcc gggcattttt
     8101 atgaacgacg taatgggtcc ctggtctttt tcgcagagga tattgatgaa cgtcaccaaa
     8161 agctaagtga ggtgtttgtt caaagtgaac atggggagac gttgcaagtc ttttcagcaa
     8221 agacagctca agagcaactt gatccccaaa gcggcatctc gattccagtt ctctttgatg
     8281 ggttcgaata taaactgaca agggaggggg agatcaagca catttccagt ttttcacgga
     8341 tggcgattta tcctcaggag cttgtggcgg agtatagacg caaggccgaa tccacccaaa
     8401 atttattaaa atctgattct cgcaaggata tcacagaact gcaatggagg tttttagccc
     8461 ctttttcggc tattctcatg gggctgctgg gagtgccatt gagtcgcgct tccccgaggc
     8521 agggcaaata cgccaaaatt ttcacagcca cggtaatttt ttcagtctat tactttacgg
     8581 ggctgatggt gagaaatctt gttgaacagg gcatactccc cattattccg ggtctttggt
     8641 ggattgttct tgttttagga gccatattgg catattggtt ggcgccctcg cgatttaccc
     8701 gagtcacgtc ctaagaaatt ctggttcata ctctctcacg atcctcagat tttctgaaca
     8761 gaggcagtaa gtcgtaaatg gcgtatctgg cgggtttcac gataatttca tgaaaattct
     8821 caatcggtat ctggcggtca gtttactgaa agggtatttt cctgtgctgg cgatattttt
     8881 ggcggttttc agtttaatcg tctttgttga agagctggat gacgtgggta aggggcgcta
     8941 taccttttgg agcgctggtg agtttttgtt gctgaccctg ccaaaccggt tggtgtttct
     9001 cgccccgttt gtgtcgttgt tagggagtat tattgcccta ggtagccttg ccaatggcag
     9061 ggaactcatc gcgatccagg ccgctggagt gtctccctac caaatagcct ggtcagtgat
     9121 gaaggttggg gcatgtttta ttttacttgt gggctttctt gaagaggttg tcaatccccc
     9181 cctggatcaa gaggcctatt ttaagcgatc aatggcattg tcggattctg gggcctataa
     9241 gggaaagcag ggtttctggt ttcgtgatgg aagacgcttt attagaattc atcagattcg
     9301 ttacggagag agtccagaaa acattgatat ttttgaattt aatgatgccg gacaattgga
     9361 tttctatatg catgctcaag aggcagaaat tgtcaaccct cagaagtgga ctctgaaaaa
     9421 tattaaaaag caggtcattc acggatatag tgtttcccag gagcagatgg agagtctgga
     9481 atgggactct ccacttaaac agaatgaagt ccggttattg actcttcctt cttccacatt
     9541 ggctccttcc gagctttacc aatacatgga agtcttaaaa cgaaaaaaac aaaattacct
     9601 gagatatgag ttaagctttt gggacaaaat ttttatgccc ttaaataccg ggcttatgat
     9661 cttggtggcc gtcccgtttg tgtttggccc cttgcgaatg tctgcagcag ggaagagggt
     9721 attcattgga tccctggtcg gtctggggta ttatttaatg acggaaattt ctaagcatgt
     9781 tggaattctg ttcggagcct ctcctctttt aaccacggcc gctcccttcc tcctgttgtc
     9841 tcttgtcacg tttgtgttat ggcggcgtta tctcaattga aaaacagtgg aaagtcggca
     9901 tagagatcgg gcatgatcaa ccgctat
//
LOCUS       DATIAL010000481         6730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_946504, whole genome shotgun sequence.
ACCESSION   DATIAL010000481 DATIAL010000000
VERSION     DATIAL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946504"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="VLA60_14755"
     CDS             <1..1571
                     /locus_tag="VLA60_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HSF10667.1"
                     /translation="SDEAGYRPRRHQIIRMLSVVRGTVTFRLECAPAFNFGRDPHDIK
                     TRPKGIIFQSGDQSVGLVSPIPLHTRENFAYQEFTLHQGESLSFFLEYLEVKNGDQLL
                     STPESGDEAFRNTSAFWQRWLAQCQYDGRWREVVHRSALALKLLTYAPTGAIVAAPTT
                     SLPENIGGPRNWDYRYTWIRDAAFSIYGLLRLGFTVEASRFMDWLNARCCELNPDGSI
                     QLMYGIDGRRNLTEEELPHLDGHRSSRPVRIGNGAASQLQMDMYGALMDAVYLYNKHG
                     ASISYDLWVNLCRLLDFVCDNWEQPDEGIWEVRGGRRHFVYSKVMCWVALDRGIRLAD
                     KRGFPGNRARWMEERDRIYREIMARGWNADIGAFVQSYDSEALDAANLIMPLVFFVSP
                     TDPRMLSTIDRTLEQLSLGSLVYRYENGKAASDGLDSEEGTFSMCTFWLVEALTKAGR
                     LTEARLIFEKMLSYGNHLRLYAEEVSHSGEQLGNFPQAFTHFGLITSACNLDLALNTK
                     RAAHTVVRRNRPSV"
     gene            1635..2528
                     /gene="gnd"
                     /locus_tag="VLA60_14760"
     CDS             1635..2528
                     /gene="gnd"
                     /locus_tag="VLA60_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HSF10668.1"
                     /translation="MDIGFIGLGKMGMNMVTRLSQGGHRVIAYDRSAALISEAETKGA
                     NPSSSLEDLITKLPKPRVVWVMVPSGQPTEETVQHLGKILEANDIIIDGGNTKFHDDV
                     RRAADLHTRGIQYIDAGTSGGIWGLQIGYCLMVGGKQEPVKRLAPIFTTLAPENGWAH
                     VGGHGAGHYVKMVHNGIEYSMMQGYAEGFELMSKSDYNLNLATIADLWMHGSVVRSWL
                     LELAAGALKKDPKLEQLQGYVQDSGEGRWMIMDAIEKDVPVPTLTTALFTRFRSRQET
                     SFAEKMLAALRKAFGGHSVRP"
     gene            2550..4100
                     /gene="zwf"
                     /locus_tag="VLA60_14765"
     CDS             2550..4100
                     /gene="zwf"
                     /locus_tag="VLA60_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSF10669.1"
                     /translation="MSPNEPSPGTEAPVTTNRTAATQSCTIVIFGASGDLTQRKLLPA
                     LYNLLLDGLLPDNFAVLGLGRKPLSDEAFRGIAREGIEQFSRQTLEPDKWKTFQSRLF
                     YCAGDISASDYYGKIRNRLKDIEAPFKLPGNRIFYLAIPPTSFAPACEGLQKAGLVAV
                     PDQADTYSRVIVEKPIGHDLSSARAINTAIGNVFDESQIYRIDHYLGKETVQNIMVMR
                     FANSIFEPLWNHKYIDHVQLTVSEAVTLGTRATYYEGAGALRDMVQNHILQLLCLIAM
                     EPPYSLDPDVVRNARMDVLRGLRPIRGQDVEKMTVRAQYAHGNIKGQEVPGYRREEGV
                     QPDSTTETYVALKVFVENWRWAGVPFYIRTGKAMPTRASEIAVQFKDIPQILFNANPQ
                     IPQAPNLLTLRIQPEEGMALRIISKVPGTKAQTHPVDMDFHYGDAFKAPSPEAYERLL
                     LDVMAGDTSLFMRRDAVEASWKWVTDILEGWSAYETKWLPEYPAGSWGPVEADRLIHA
                     GGHHWRKI"
     gene            complement(4297..>6730)
                     /locus_tag="VLA60_14770"
     CDS             complement(4297..>6730)
                     /locus_tag="VLA60_14770"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HSF10670.1"
                     /translation="PQGLPAIEELLSQGININVTLLFSVVVYEQVAWSYIRGLKRLAA
                     HRGNLHRIASVASFFVSRIDTLIDKKLDAKISKESRPAQRAMLESLLGKVAIANAKLA
                     YQIFQEVFASSEFQPLKAEGARVQRVLWASTGTKNPKYPDTYYVDNLIGPDTVNTMPE
                     ATFTAFRTHGTVKNTIQEGLADAKITMQQLAEAGISMEEVTDTLLKDGAQLFLDAFDQ
                     LMGVISRKRAIILGEKVDRVTYSVGPLQPKIDAALKEFQHTNLVRRLWSKDPTVWHHD
                     PAHQEIIRNALGWLRITEQQLSSIPRLLALAKEIKEAGFQHVLILGMGGSSLCPEVCR
                     MTFGLIPGYPELHVLDSTVPSQVRAFEHRVELAKTLCVVASKSGSTTEPLVFQKYFFE
                     RMRQVVGEKAGNHFVAITDSGSLLEQMAKELRFRHILPGVPEIGGRYSALSNFGMVPA
                     ALMGVNIEHFLYRAERMRHSCGASVPPQDNPGVILGTVLGMCAKARKDKLTFVTSPAL
                     WDLGAWLEQLVAESTGKEGKGIIPVDGESLGDPGVYDQDRVFAYIRYEQGIDVTQEAK
                     VKALEAAGHPVIRINVADLINLGEQFFLWEMATAVAGALLEINAFDQPNVQESKDYTK
                     KYLEAFTRNGTLPDSPPFLTDGNVTVYTDEQNASVLQGKASLEAVLGAHLARIQSGDY
                     FAINAYVERTDPVHAIFQRMRTNIRENKQVATTLGYGPRFLHSTGQLHKGGPNSGVFI
                     QVTCDDREDLPIPGEPYSFGVLKAAQALGDMQSLSSRHRRVIRLHLEADVEKGLARLE
                     QLIEASIGSHAH"
BASE COUNT         1651 a         1821 c         1682 g         1576 t
ORIGIN      
        1 aatcggacga agccggatac cggcctcgcc gccaccaaat tatccgaatg ctctcagtgg
       61 tccgtggaac cgtcaccttt cgacttgaat gcgcgcccgc atttaatttc ggccgggatc
      121 ctcatgacat caaaaccaga ccaaaaggca tcatctttca atccggagat caatccgtgg
      181 gattggtcag ccccattccc cttcatacgc gggaaaattt cgcgtatcaa gaattcacac
      241 tccatcaggg cgaaagcctg tcattttttt tggaatactt ggaagtcaaa aacggcgacc
      301 agttgttatc cacccctgaa tccggcgacg aagcctttcg aaatacatcc gccttctggc
      361 agcgctggct ggcacaatgc caatacgatg gacgatggcg ggaagtggtt caccgttccg
      421 ccttagccct caaactgctg acctatgccc ccacgggagc cattgtggcg gctcccacaa
      481 ccagtcttcc cgaaaatatc gggggacccc gaaattggga ctatcgttat acctggattc
      541 gggatgcagc cttttctata tacgggctat tacgcttggg atttacggtg gaagccagtc
      601 gattcatgga ttggctcaat gcccgttgtt gcgaactcaa tccggacgga tccatccaat
      661 tgatgtatgg cattgacggt cgtcgtaatc tgaccgaaga agaattgccc catctggatg
      721 gccaccgcag ttcccgtccc gtccgcatcg gcaatggagc cgcgtctcaa ctgcaaatgg
      781 atatgtacgg cgcactgatg gacgcggtat atctctataa taaacacggc gcgtccattt
      841 cctatgatct ctgggtaaac ctttgccgcc tcttggattt tgtctgcgac aattgggagc
      901 aaccggatga aggcatctgg gaagtcagag gaggacgccg tcattttgtc tattccaaag
      961 tcatgtgttg ggtagcctta gatcgcggca ttcgtctggc cgacaaacgg ggctttccag
     1021 gcaaccgggc ccgatggatg gaggagcgcg accggatcta ccgggaaatt atggcacggg
     1081 gttggaatgc cgacattgga gccttcgttc aatcctacga tagcgaagcg ttggatgccg
     1141 ccaacctgat tatgcctctc gtcttctttg tgtccccaac cgatcctcga atgctctcaa
     1201 ccattgaccg cacactcgag caactgtcat tagggagcct cgtgtatcgt tatgaaaacg
     1261 gaaaggccgc ctcagatggc ctggacagcg aagaaggcac ctttagcatg tgcacctttt
     1321 ggttagtcga agccctgaca aaagcggggc gattgacgga agcacgactg atctttgaaa
     1381 aaatgttaag ttatggtaat catcttcgtt tatatgccga ggaagtctcg cactcgggtg
     1441 aacagttagg aaatttcccc caggccttta cacattttgg tctcataacg tcggcgtgca
     1501 atcttgatct ggctctcaat acgaagcgtg ccgcccacac tgtcgtgagg cgtaatcgac
     1561 cgtcagtatg actctcacgc caggattaat gacgactgaa aggaccacca ctcgacatca
     1621 caggaggatc gcatatggat atcggtttca ttggacttgg caaaatggga atgaacatgg
     1681 tcacccggtt aagccaggga ggacatcggg tcattgccta cgaccggtcg gccgccctga
     1741 tcagtgaggc cgaaaccaaa ggggcgaatc catcttcatc cttggaagac ctgatcacca
     1801 aattacccaa gccgcgagtc gtctgggtca tggtcccctc aggtcaaccc accgaagaga
     1861 ccgtacaaca tctcggaaaa atcttggagg ccaacgacat catcattgat ggaggcaata
     1921 cgaaatttca tgatgacgtt cgccgggcag cagaccttca cacccgtggc atccagtata
     1981 ttgatgccgg aaccagtggg ggtatttggg ggttgcaaat cggctattgc ttgatggtcg
     2041 gtggaaaaca ggaacccgtc aagcgattgg ccccgatctt cacaaccttg gctccggaaa
     2101 atggatgggc ccatgttggg ggacatggcg ctggacatta tgtgaagatg gttcacaatg
     2161 gcattgaata cagtatgatg cagggatacg cggaagggtt cgaacttatg tccaagagtg
     2221 actataactt gaatctcgcc acaatcgccg atctctggat gcatggcagt gtggtcaggt
     2281 cctggctgct tgaactcgct gccggagctc tcaaaaagga tcctaagctg gaacaattac
     2341 aaggctacgt gcaggattcc ggcgagggac ggtggatgat aatggatgcc attgaaaagg
     2401 acgtaccggt tcccactctg acgaccgctt tattcacccg attccgctcg cgacaggaaa
     2461 cctccttcgc ggaaaaaatg ttagcagccc tgcggaaggc ttttggcgga catagcgtcc
     2521 gaccataacc ggattcttgc gaggtcgaca tgtcaccgaa tgaaccttct cctggaaccg
     2581 aagcccccgt cacgaccaac cgaaccgcag cgactcaatc gtgcacgatt gtaattttcg
     2641 gtgcctcggg cgatctaacc cagcgtaaac ttctgcccgc cctctacaac ctcctcctgg
     2701 acggcttgct accggacaat ttcgccgttc tgggattagg ccggaagcct ctttcggatg
     2761 aggcttttcg cggaattgcc cgtgagggca ttgaacaatt ttcccggcaa acgctcgaac
     2821 ccgacaagtg gaaaacattt caaagtcgat tgttttattg tgcaggagat atttccgcct
     2881 ccgactatta cgggaaaatc cggaaccggc tgaaagacat tgaggcacca ttcaaactac
     2941 ccggcaatcg tattttttat ttagccatcc cgccaacctc ctttgcaccc gcatgcgagg
     3001 ggctccagaa ggccgggtta gtggccgtcc ctgaccaagc ggacacgtac tcccgggtca
     3061 ttgtggaaaa accgattgga cacgatttgt cttctgcccg agcaattaat acagccatcg
     3121 ggaatgtctt cgatgaatcg caaatttatc gcatcgatca ttatctgggg aaggagaccg
     3181 tccagaatat tatggtcatg cggtttgcca actccatctt tgaaccgcta tggaaccata
     3241 aatatatcga ccacgtgcaa ttgaccgtca gcgaagccgt cacccttgga acccgagcca
     3301 cctattatga gggggcagga gcccttcgag acatggtgca aaatcatatt ttgcaactgc
     3361 tctgcctgat cgcgatggag cccccctact cactggatcc ggatgtagta cgaaatgctc
     3421 gaatggatgt gttacgggga ctgcgcccaa ttcgcggaca agacgtcgaa aagatgactg
     3481 ttcgcgccca gtatgcccat ggcaacatca agggccagga agtccctggc tatcgacgag
     3541 aggaaggggt ccaaccggac tccaccacgg aaacatatgt ggccttaaaa gtttttgtgg
     3601 aaaactggcg ctgggccgga gtgccattct atattcgcac gggaaaagcc atgccgacgc
     3661 gcgccagcga aatcgccgtc caattcaagg acattcccca gattttattt aatgccaatc
     3721 cgcaaattcc tcaggctccg aatctcctca cgcttcgtat tcaaccagaa gagggcatgg
     3781 cccttcgcat tatttccaaa gtaccgggaa ctaaagccca aacgcatccg gttgatatgg
     3841 actttcacta cggtgatgcc tttaaggcgc cttccccgga agcctatgaa cgattgctgt
     3901 tggatgtcat ggcgggggat acgtccttgt tcatgcgacg ggatgccgtt gaggcttcgt
     3961 ggaaatgggt cacggatatc ctcgaagggt ggagtgcata tgaaaccaaa tggttaccag
     4021 aatatcccgc cggtagctgg gggcccgtcg aagccgatcg actgattcat gcaggcgggc
     4081 accattggcg aaaaatttag gccattcctg ctcttaattt ttctcgcaca aatgacgcga
     4141 cgggagaaga gtatccccgc tcccctgtcc acccaaccgg ttcaacgtcc tcatacctga
     4201 gcgtgactaa gagcctgtca aacgttgggg gatccaggac gaaatatgga tcgcttggcc
     4261 catgtgcagt tgactcatcc gaagtccggt aggctcctag tgagcatggg aaccgatgct
     4321 ggcttcaatc aattgctcca ggcgagccaa ccccttttca acatcggcct ctaggtgaag
     4381 acgaatgacc cgtcggtgac gactgctcag gctttgcata tcgcccaatg cttgcgcggc
     4441 tttcaacacg ccaaacgaat atggttcacc aggaatggga agatcctccc gatcatcgca
     4501 tgtcacctga ataaaaactc cagaattggg tccaccctta tgcaattgcc ccgtggaatg
     4561 gagaaatcgt gggccatatc ctaacgtggt tgctacctgt ttgttctcac gaatattggt
     4621 gcgcatgcgt tgaaaaattg catgaaccgg atcagtacgt tccacgtagg cattgatggc
     4681 aaaataatca cctgattgga tacgggcgag atgcgcgccc agcacggctt ccaaggaagc
     4741 cttgccttgg aggacagagg cgttctgttc atcggtatag acggtcacat tgccatcagt
     4801 caggaaaggg ggggaatccg gcaaggtgcc gttccgggtg aaggcttcta aatatttctt
     4861 tgtgtaatcc ttactttcct gaacgttagg ctgatcgaac gcattaatct ctaacaaagc
     4921 tccggccacc gccgtcgcca tttcccataa gaaaaattgc tcaccaagat taatcagatc
     4981 ggcgacgttg atgcgaatca ccggatggcc ggctgcctca agtgctttta ctttagcttc
     5041 ctgtgtgaca tcaattcctt gttcatatcg gatataggca aagacccggt cctggtcgta
     5101 gaccccaggg tctcctaacg attccccatc aacgggaata attccctttc cttctttccc
     5161 cgtgctttca gccaccaatt gttctaacca cgcacccaga tcccatagtg ccggcgaggt
     5221 cacaaacgtc agcttgtcct ttctagcctt ggcacacatg cccaggaccg tcccgaggat
     5281 cacacctgga ttatcctgag gcggcacgct ggccccacag gaatgacgca tgcgttccgc
     5341 ccgatacaag aaatgctcaa tattcacgcc catcaaagcc gcagggacca taccaaaatt
     5401 cgagagagcc gaatatcgcc cgccaatttc tggaacaccc ggcagaatat gtcggaatcg
     5461 cagctccttg gccatttgct ccagcaacga gccagaatcg gtaatggcca caaagtgatt
     5521 gcccgccttc tccccgacaa cctgccgcat acgctcaaag aaatattttt gaaacacgag
     5581 gggttcggtt gtggatcccg atttactggc taccacacac aacgtttttg ccaattcgac
     5641 ccgatgctca aatgcccgca cctgcgaagg taccgtacta tccagaacat gtaattcggg
     5701 atatcccgga attaagccaa aggtcatccg gcagacttcc ggacacaagc tgcttccccc
     5761 catgcccagg atcagaacat gttgaaaccc cgcttctttg atctcttttg ctaaagccag
     5821 caatcggggt atcgaggaaa gttgttgttc tgtgattcgc aaccacccca aggcattccg
     5881 aatgatctcc tgatgggcgg gatcgtgatg ccagacagta ggatctttac tccacaaacg
     5941 gcgaaccaga ttggtatgct gaaattcttt gagcgccgcg tcaatcttcg gctgcagagg
     6001 gccaaccgaa taggtaaccc ggtccacttt ttcccctaaa atgatggccc gtttccggct
     6061 aatcacgccc attaattgat caaaggcatc caaaaacaat tgtgcgccat ccttcaggag
     6121 agtgtcggtg acctcctcca ttgaaattcc agcttcggcc aactgctgca tggttatttt
     6181 cgcgtcagcg aggccctcct gaatcgtatt cttcacggtt ccgtgcgttc ggaatgccgt
     6241 aaacgttgct tcaggcatcg tgttcacggt atccggaccg atgagattat caacataata
     6301 ggtatccggg tatttggggt ttttcgtccc ggtgctcgcc caaaggaccc gctggactcg
     6361 agcgccttct gctttgaggg gttgaaattc tgagctcgca aagacttcct gaaaaatttg
     6421 ataggccagc ttggcattgg caatggcgac ctttcccaga agactttcca gcatcgctcg
     6481 ttgggcagga cgggattctt ttgaaatttt tgcgtctaat tttttatcaa tcagagtatc
     6541 aattcgactg acgaaaaaac tggccactga ggcgatacga tgcaaattcc ctctatgcgc
     6601 tgccaaccgt ttcagcccac gaatataact ccaggccacc tgctcatata ccaccacact
     6661 gaataaaaga gtgacattga tattgatgcc ttgggacaga agttcctcaa tggcgggtaa
     6721 cccctgaggg
//
LOCUS       DATIAL010000482        13960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_838773, whole genome shotgun sequence.
ACCESSION   DATIAL010000482 DATIAL010000000
VERSION     DATIAL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838773"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="VLA60_14775"
     CDS             <1..2186
                     /locus_tag="VLA60_14775"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSF10671.1"
                     /translation="SSAFGGPIPMPTADRLANNGLRYNHFHTTALCSPTRSALLTGRN
                     HHMTNTGSIMETATAFPGNTGQRPDSVAPLAMMLRYNGYSTAQFGKNHETAAWEVSPS
                     GPTDRWPTRSGFDKFYGFMGGETNQWSPAVYDGMTKVEVSKDPNYHFMTDMTDQAIKW
                     MRFQKSLTPDKPFFMYFAPGATHAPHHVPQEWIAKNKGRFDQGWDKVREETLARQIKL
                     GVVPEGTMLAPKPKAIKDWDNLTADEKKLFTRQMEVFAGFAEYTDTEVGRLIDAIHDM
                     GQLDNTLVFYILGDNGASAEGGMNGMFNEMTYFNGLQEKVEDILKHYDTLGGPTTYNH
                     YAAGWAVAGDTPFTWTKQVASSYGGTRNGMIVHWPKSVKAKGELRTQWHHVIDIAPTV
                     LEAARLPEPKAVNGIPQTPIEGVSLLYTFHDDKAQDRHQTQYFEIFGNRAIYHEGWLA
                     STLHRAPWELKPRRPLQEDKWELYDTRTDFSLINDLSDKHPAKLKELQDLFLKEAVKY
                     HVLPIDDRTIERVNAALAGRPDLMAGRTSLSVYEGMTGMSENVFINTKNRSHTITADV
                     EIPEGSVNGVVLAQAGRFGGWSLYLKEGKPTYTYNFLGLMRFTIAGDDPLPAGKASIR
                     FEFAYDGGGLGKGGTGTIFVNDKKVAQGRIEHTQGMIFSADEGADVGEDGETPVVEDY
                     GIPPPYRFSGSVHKVTIDLKEMKRTDKAAEERGQAIARHKIAIAD"
     gene            2568..4103
                     /locus_tag="VLA60_14780"
     CDS             2568..4103
                     /locus_tag="VLA60_14780"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HSF10672.1"
                     /translation="MKPFLTLLTLAVVLTLSGLTTTQAAETKPNILVIWGDDIGGFNV
                     SAYNMGMMGYKTPNIDRIAKEGALFTDWYGQQSCTAGRAAFITGQSPIRTGLTKVGLP
                     GAPEGMKKEDPTIATLLKAQGYMTGQFGKNHLGDRDEMLPTNHGFDEFFGNLYHLNAE
                     EEPEHPDYPKNPDFKKRFGPRGVIHSFADGRITDTGPLTKKRMETIDEEVNVKALDFM
                     ERAKKSDKPFFLWWNSTRMHIFTHLKKESAGKTALGIYADGMVEHDGHVGQLLAKVKE
                     LGLEENTIVMYSTDNGSESFSWPDGGTTMFRGEKNTQWEGGYRVPTAIKWPGVIQPGT
                     VINDIGAHEDMLPTLLAGAGDISAKEDLLKGRTIGNITYKVHIDGYNLLPAFKGEGQW
                     PRKEFIYWTDDGNVAALRYGNWKATFLRQDAHGLHVWLEPFVVLRAPLLTNLRMDPFE
                     LAHEIGMDYERWFAEHMFMIAPAAGYIGQWLQSFREFPPRQKPGSFNLDNVMKSVTSG
                     SKQ"
     gene            4207..4587
                     /locus_tag="VLA60_14785"
     CDS             4207..4587
                     /locus_tag="VLA60_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10673.1"
                     /translation="MSLRSLFILTAILEAGAGLALTLSQSASTVVLVGGSLDTDGGLI
                     AARIAGAALVSLGVACWFARNDGQSDAAKGLVSAMLLYNVGAGGVFLYAGMGLGLTGI
                     RLWPAALLHTGLASWCLGCLRSPM"
     gene            4644..5681
                     /locus_tag="VLA60_14790"
     CDS             4644..5681
                     /locus_tag="VLA60_14790"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF10674.1"
                     /translation="MKTNRTHTFVAAGFCCIALFPATQIYAADELPSWRDGMNKQAVV
                     DFVKRVTTPGPDFVPEAARIAVFDNDGTLWAEQPMYFQLFFALDRVKALAPQHPEWKT
                     TEPFASLLKGDVRGALAGGEHAILKIIMATHAGMTTAEFETIVKDWIATAKHPKTERL
                     HTEMVYQPMLELLAYLRTNGFKTFIVSGGGIEFMRPWTEQVYGIPPEQVIGSSGKLKY
                     AVRDGDPVLVKLPKINFIDDKDGKPVGIQTHIGRKPILAFGNSDGDLQMLQWTASWSG
                     ARFMALIHHTDAEREWAYDRKSSIGHLDKALDEAKSKGWTVVDIKQDWKAVYPFHRHS
                     GDVSLSESSGR"
     gene            6406..7026
                     /locus_tag="VLA60_14795"
     CDS             6406..7026
                     /locus_tag="VLA60_14795"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HSF10675.1"
                     /translation="MRNWSRGIFLGHLTSLERLNALTDGVYAIVITLLVLDLKVPETP
                     GLTTGQLLIDLQEQIPNFAAWILSFYMAAFLWMRNFWILKHLDKCDEKTFWLNFVHLM
                     FVSLIPYAASLLGHYEEDAVAVIIFSSTLGLASFSLLILHRYAAGKREWHLNGAPKLW
                     TNPNLWAMYPAPIFAIGSILISFNNVNGAIAFWFLAPLWAHFFSQR"
     gene            complement(7078..7263)
                     /locus_tag="VLA60_14800"
     CDS             complement(7078..7263)
                     /locus_tag="VLA60_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10676.1"
                     /translation="MTVLSVLEASAPEKKGEGCLISVLPHDPVKTRSGEAQAKITTAQ
                     HTAIVNQSRPKHTCPRR"
     gene            7520..7858
                     /locus_tag="VLA60_14805"
     CDS             7520..7858
                     /locus_tag="VLA60_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10677.1"
                     /translation="MGAEEEELKIKIRRLVKEKFEGDFRKGFDHYDSIDGQDLKIGSS
                     ALEQLLADAKIGNFLTRTLWVEGVLKALDKDQDGAISWDEFASILEDSPQTKEKAARD
                     PDDDSSPANK"
     gene            8777..9244
                     /locus_tag="VLA60_14810"
     CDS             8777..9244
                     /locus_tag="VLA60_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10678.1"
                     /translation="MTNIFAMVLTLAMGIIGTSLAANEEMHPLPFIITDIERHIAELT
                     INIEKVSDRMKFLREVPDSKDPLIQEVRNLDLRGWELHQEQWKLQLDGLRFTEGLLRK
                     VQEHPEEKPDALQAWLARLKGFKEAMNDYRQQRAGIEVLRIETESKLIEQYLR"
     gene            9260..9841
                     /locus_tag="VLA60_14815"
     CDS             9260..9841
                     /locus_tag="VLA60_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10679.1"
                     /translation="MDCVKRPTVLFALCLTGIWFIMTGCTSVDTILLTSQTFPPKKST
                     LDVEVMALEPKCPHIALAQLSVLDSAESYGDEEGAIIKKAADLGADAVVLYKGTKRTV
                     ATPAYGGYAYNYGYPGWGYASYGMGGYGGYGTTGYTIGGFGYSPYGMGTPMMYDTTVR
                     SLSGIAIRYQDGPRCVPGKPESRQIHTSFPLQS"
     gene            complement(9979..10338)
                     /locus_tag="VLA60_14820"
     CDS             complement(9979..10338)
                     /locus_tag="VLA60_14820"
                     /inference="COORDINATES: protein motif:HMM:NF028504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5132 domain-containing protein"
                     /protein_id="HSF10680.1"
                     /translation="MVFDLLEFPHASLNLSGYINLLTISKKYFIYKGGAHGSCHWLCG
                     GRSCCAVAVPSMASGIVSVLCPLGKEIIQGGLVAYATVSEMVSETGEHFNDIVAEVKA
                     EIEHHDQCLEPIVGSGL"
     gene            complement(10486..11022)
                     /locus_tag="VLA60_14825"
     CDS             complement(10486..11022)
                     /locus_tag="VLA60_14825"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HSF10681.1"
                     /translation="MRLFVLLALPGFWGCSTISVSSDYRESTDFNELKTFSWMLKTPE
                     GDIDLGGANQMARQRIQDAVAAELANKGYIEIIAGNSDFHVTYYVGREERIQVDTMRG
                     PLMRPYWGMGMGYTEVYQYEEGTLIIDLLDAKPPQHIIWRGVAKGVVDWKGTTGGQTG
                     LINEAVQKVLAQFPPKRS"
     gene            11524..12606
                     /locus_tag="VLA60_14830"
     CDS             11524..12606
                     /locus_tag="VLA60_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10682.1"
                     /translation="MNIRRQLRTRQRLFLKCIGTGDPAYMPFFVSLLIGLLFPASPSF
                     AEWRVTGESNVYYTDDAALFSSTRRLSRNQDPTQPVIDSLLANQGDDVVFEPMAHIMK
                     SFSMMGRPTELGVRGQGFVFTDNTQFNHGSLSVEASHNLTSTSALLMRYFFGPDLFLG
                     KNEVRPLEESEGNPPQVKNEEVTTNYWAAGLAQQIPGVDDLRLILYGRYGLRDYDKPF
                     RQRDTRFWTIGTHVEWAPTQKIGLALGYHYERGLADGRKQPELQDDTSYFNHFVTGEF
                     DYEITERLSLEMGLHYEFNGWTTGIQGDERKGQHENVIQGDIASRYRVNEILQITAGF
                     QGSHRKESFEKGLKNLNTWLGAKLTF"
     gene            12985..>13960
                     /locus_tag="VLA60_14835"
     CDS             12985..>13960
                     /locus_tag="VLA60_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HSF10683.1"
                     /translation="MQKWFTQIRNAGKVMPLSMCWFLLTASPSLAEEPHKKPTSPPED
                     PYLLWRLDAARKKVQEVPQNLKGRIRNSQEWNWDEYPDQVLLQEDTEIKWTRYLSAAL
                     NAPKWLDLGMTVRLRREGFDFPFKTEQVGSTWQWATRSRFRASARWKAFRVQLEFQGS
                     TSGVDSATDVVGNTTFSAGNVQQLFVAHTLPNFWQTGLRTDLHVGRINLDIGSRRLVA
                     RSRFSNTSQAFDGIHWNLADEDQWQFRTFFSEVVLATDTTNRLGLFTNDGNYFWGVSY
                     ETAQFAWSRIHMYYFGRKDNAGDNQVERAHSTFGLRLYQSPQVGSFDYD"
BASE COUNT         3531 a         3630 c         3577 g         3222 t
ORIGIN      
        1 agtccagcgc tttcgggggg cccattccca tgccgaccgc cgaccgattg gccaataacg
       61 gtctccggta taatcatttc cataccacgg cactctgttc cccgactcgt tccgcgcttc
      121 tgacgggccg gaatcaccac atgaccaaca cgggatcgat catggaaacc gcgacggcct
      181 ttccgggcaa taccggccaa cgtccggaca gcgtggctcc attggccatg atgctccgat
      241 acaacggtta ctccaccgcg caattcggca aaaatcacga gactgctgcc tgggaagtca
      301 gtccatccgg accgaccgac cgctggccga cccggagcgg gttcgacaag ttctacggat
      361 tcatgggcgg agagacaaac cagtggtcgc ctgctgtcta cgatgggatg accaaagtgg
      421 aagtatcgaa agatcccaac taccatttca tgaccgacat gaccgaccag gccatcaagt
      481 ggatgcgatt tcaaaagtca cttacccctg acaaaccgtt ttttatgtat tttgcacctg
      541 gagccacgca cgccccgcat catgtgcctc aggagtggat cgcaaaaaac aagggcaggt
      601 tcgaccaggg gtgggataag gtgcgggaag agacactggc tcgtcagatc aagctgggcg
      661 tcgtgcctga gggcacgatg cttgcgccga aaccgaaagc cattaaggat tgggacaatc
      721 tgacagcgga tgagaagaag ctctttaccc ggcagatgga ggtctttgcg ggttttgccg
      781 aatacacgga taccgaggtc ggacgtctca ttgacgccat tcatgacatg ggacagctcg
      841 acaataccct cgtcttctac atcctcggcg ataacggggc gagtgcggaa ggcgggatga
      901 acggcatgtt caatgagatg acttacttta acgggctcca ggagaaggtc gaggacatcc
      961 tgaaacatta tgatacgctc gggggcccga cgacctataa ccattatgcc gccggatggg
     1021 ccgttgcggg ggataccccg ttcacctgga ccaagcaggt tgcctcgagc tacgggggaa
     1081 cgcggaacgg gatgatcgtc cattggccaa agtcggtgaa ggcgaagggc gagttgcgca
     1141 cgcagtggca tcatgtcatt gatatcgcac caaccgtcct ggaggctgcc cgcttgccgg
     1201 aaccgaaagc ggtgaacggc attcctcaga ctcccatcga aggcgtgagc ctgctctaca
     1261 cctttcatga cgacaaggcg caagaccgcc accagaccca gtacttcgaa atattcggca
     1321 atcgtgcgat ctatcacgag gggtggctgg cgagtacact gcatcgagcg ccatgggaat
     1381 tgaagccccg caggcctctt caagaggata aatgggaact ctacgacacg cggacggact
     1441 tcagtttgat caatgacctg tccgacaagc accccgccaa actcaaagag ttgcaggacc
     1501 tgttcttaaa agaagcggtg aagtatcacg tgcttccgat tgatgatcga acgatcgagc
     1561 gcgttaatgc cgctcttgcc ggcagaccgg atctcatggc cggacggacg tcactctccg
     1621 tgtatgaggg gatgacgggc atgtcggaaa atgtcttcat caacaccaaa aatcgatcgc
     1681 ataccatcac agccgacgtg gagattcccg agggaagcgt caatggggtg gttcttgctc
     1741 aggcaggccg gttcggcggc tggagcctgt atctgaagga gggcaaaccg acctatacct
     1801 ataacttcct tggcttgatg cgctttacca ttgcgggcga tgatccgctg cctgccggga
     1861 aggcgtcgat ccgcttcgag ttcgcgtatg acggcggcgg tttgggcaag ggaggaacgg
     1921 ggacaatttt cgtcaatgac aagaaagtgg cccaaggacg gattgaacac acgcagggaa
     1981 tgatcttttc ggctgacgaa ggagcggatg tgggtgagga cggagaaacg cccgttgttg
     2041 aggactatgg cattccgccg ccctatcggt tttctggatc ggttcacaaa gtgaccatcg
     2101 atttgaaaga aatgaaacgg accgacaaag ccgcggagga gagaggccag gcaatcgcca
     2161 gacacaagat agccatcgcc gactgagcct atatttccag aatggactgt tggccctgac
     2221 agacgaaatc tatgccattg ttctgaccat tttggttttt agcatcgatg tgcattctaa
     2281 gtggtactct gtcccttacc aaggatggtt gttttgcgaa agaagatgat ccagcttaaa
     2341 aaagaacgtg cccaagggcg actcgtatct ttacaccgat cagtacggcg cacgatacat
     2401 cgcagacggc cgtggtaagg gcgttaccag caccaaatat tttcgattac aactggtacg
     2461 ggtagagcat tgatccctgg acgttaccca aatatatcaa cacacctgat ctgaaatcgg
     2521 aaggggaaat tgaccagagg caacccacca ctaaaggaga aaccgaaatg aaaccattcc
     2581 tcaccctcct cactctggcg gtcgtgctga cactgagcgg actgacgaca acacaggccg
     2641 ctgaaacaaa gcccaacatc ctggttatct ggggggatga catcggcggg ttcaatgtca
     2701 gcgcctataa tatgggcatg atgggctaca agaccccgaa catcgaccgc atcgcaaaag
     2761 aaggcgcgct atttactgac tggtatgggc aacaaagctg caccgccggg cgcgcagctt
     2821 tcattaccgg ccagtcaccc atacgcacgg gcctgactaa ggtcggcctt ccgggtgctc
     2881 ccgaggggat gaagaaggaa gatccgacga tcgccacgtt actcaaagcg cagggctaca
     2941 tgaccggcca gttcggcaag aaccatctcg gtgaccgcga tgagatgctg ccgacaaacc
     3001 acgggttcga cgagttcttc ggtaacctgt atcacttgaa tgccgaggaa gagccggaac
     3061 atccggacta tcccaagaat ccggatttta agaagcggtt cggcccacga ggtgtcattc
     3121 acagcttcgc cgatgggcgc atcacggata ccggaccgct gaccaaaaag cgcatggaga
     3181 cgatcgatga agaggtaaac gtcaaggcac tggatttcat ggagcgtgcc aaaaagtcag
     3241 acaaaccgtt cttcctctgg tggaactcca cccgcatgca catcttcaca catctaaaga
     3301 aagaatctgc cggaaagacc gccctgggga tctatgcgga cggcatggtc gagcatgacg
     3361 gccatgtcgg tcagcttctc gcaaaggtca aagaactggg cctcgaagag aacaccatcg
     3421 tcatgtattc caccgacaac ggctccgagt ccttttcctg gccggatggg ggcacaacga
     3481 tgttccgtgg ggagaagaat acgcaatggg agggcggcta tcgtgtgccg accgcaatca
     3541 agtggccggg cgtaatccag cccggtacgg tcatcaacga catcggggca catgaagata
     3601 tgctcccgac cctgctcgca ggggcaggcg acatctcagc caaagaagac ctgctgaagg
     3661 gacgaacaat cggcaacatc acgtacaaag tgcacatcga cggctataat ctcctgcccg
     3721 cgtttaaggg cgaaggccag tggccacgca aggaatttat ctactggacc gacgacggga
     3781 acgttgcggc gcttcgctat gggaactgga aagccacctt cctgcggcag gatgctcacg
     3841 gcctgcatgt ctggctggaa ccattcgtcg tgctacgggc cccgctgctg accaatctgc
     3901 gcatggaccc cttcgaactc gcccacgaaa tcgggatgga ttatgagcgc tggtttgccg
     3961 agcacatgtt catgatcgcc ccggcagccg gatacattgg acaatggctg caaagcttcc
     4021 gtgaatttcc gccgcgccag aagcccggca gcttcaatct tgacaacgtg atgaaatccg
     4081 tgaccagcgg ctcaaaacaa taatgttgag ctgcctgacg atgtggtaga ccgggcttca
     4141 gtccgcatcc gcctctccgg cggatgcgga ccaacacacc aaccactcaa ttggaatggc
     4201 acctctatgt cactcaggtc tttgttcatc ctgacagcca tccttgaggc aggggcaggt
     4261 ttggcgctaa ctctctcgca gtcggcatcg actgtggttc tggttggtgg ttccctcgat
     4321 acagacggcg gtctcatcgc agctcgcatt gcaggagccg cgctcgtttc cctcggtgtc
     4381 gcctgttggt ttgcacgcaa tgacggacag agcgacgcgg cgaaagggtt ggtgtcggcc
     4441 atgttgctct acaacgttgg tgccggcgga gtcttcctct atgcgggcat gggcctggga
     4501 ctgaccggca ttcgtctctg gccggcagct ctccttcaca cagggttggc cagctggtgc
     4561 cttggttgcc ttcgatcacc tatgtgaacc tgatcagggg aacgtcgtcc aagatcgatt
     4621 tcaaacacag aaaggaaccc tggatgaaaa caaaccgtac gcatactttc gttgccgccg
     4681 gtttctgttg catcgcgctt tttcccgcaa cccaaatcta cgccgcagat gagctcccct
     4741 cctggagaga cggtatgaac aaacaggccg tcgtggattt tgtcaaacgt gtgaccacac
     4801 ccggtcccga ctttgtgcct gaagctgctc gcatcgccgt ctttgacaat gatggcacgc
     4861 tttgggcgga acagcccatg tacttccaac tgttctttgc gcttgaccgc gtcaaagcac
     4921 tcgcgccaca acatcccgaa tggaaaacca cggagccgtt cgcctctttg ctcaaagggg
     4981 atgtaagggg cgctctggcg ggcggtgaac acgccattct caaaattatc atggccaccc
     5041 acgcggggat gacgacggct gagttcgaga caatcgtaaa agattggatt gcgaccgcga
     5101 agcatcccaa gaccgaacgt ctgcacactg aaatggtgta tcagcccatg ttggagttgc
     5161 tggcgtatct tcggacgaat ggtttcaaga cgttcattgt ttccggcggc ggcatcgagt
     5221 tcatgcggcc gtggacggag caggtgtatg gcatcccacc agaacaggtt atcgggagca
     5281 gcggcaaatt gaaatatgcg gtgcgcgatg gggatccggt actcgtcaaa cttcctaaaa
     5341 tcaatttcat tgacgataag gacggcaaac ctgtcggcat tcaaactcac atcggacgga
     5401 agcccattct tgccttcggc aactcagacg gcgatcttca aatgctccag tggacggcaa
     5461 gctggagcgg agcgcgcttc atggctctca tccaccacac ggacgctgag cgtgaatggg
     5521 cctatgaccg caagtcatcc atcggccatc tggataaggc acttgacgaa gcgaaaagca
     5581 aaggatggac ggtggtagac ataaaacagg attggaaggc cgtttatccg tttcacaggc
     5641 atagcggcga tgtgtctttg tccgaatcct cgggtagatg aatttcatat ggccgattta
     5701 tcggagggat gatccaagca aagttcacat tgatggaaat cgctttccta tagaaacgca
     5761 tttggtccac caaaaccggt cggctacctg ccgggcatcg gtgtattggt gcaaaatggg
     5821 aaacgcaatc ccgtcctgga taagcccggg acagggatcg aacaaaaacc tggggatcga
     5881 attccgatga aggaggaaaa cctacggtcc ggtctacaaa atgaaaacct tcctgacaaa
     5941 ggacacgaac catttctctt tttatcacgg atcattgact agacatcctg ttctcaagaa
     6001 gtgccgtggg tcattctgaa agaacacatt gaggtttctg aacggcaagt ccagaggttt
     6061 gtccgcactt tcactccaaa cgccccgccc tactctgaag agctgtttat aaaaatcgcc
     6121 tcttcctgca ttggactctt cacgctattc caacttattt aattctcatg ccgttcctcc
     6181 atatctattc cgacgtaatt cgggagtgac cggcaaatga gcaaacggcc ggagtggcac
     6241 tcccggtctt ctatgaggta atatttccac attgcattgg aagatttcat gggttgtcat
     6301 gccgggcaac cacatcaccg tcgcacaata catcactcac actccttgat actcgagaca
     6361 aaagaggtgt gagcatccac tcattttcaa ggacaacaca ggcttatgag gaattggagt
     6421 cggggtatat ttttaggcca tctcacctca ctggaacggc tcaatgccct cactgacggc
     6481 gtgtacgcaa tcgtgatcac ccttctggtg ctggatctga aggtgcctga aactccgggg
     6541 ctgaccacag ggcagcttct tatcgacctg caggaacaga tcccaaattt tgctgcatgg
     6601 atactcagtt tttatatggc ggcgtttctg tggatgagga atttttggat tctcaagcac
     6661 cttgataaat gcgatgaaaa gacattctgg ttaaactttg ttcatctgat gtttgtatcg
     6721 ctcatcccct acgcggcctc gttgctcgga cattacgaag aagatgccgt tgcggtcata
     6781 attttctcct caactcttgg tctggccagt ttttccttac tgatcctcca ccggtatgcg
     6841 gccggcaaac gcgaatggca tctgaatggt gctcccaagc tctggacgaa cccgaactta
     6901 tgggccatgt accccgcacc aattttcgcg ataggttcta ttctcatctc tttcaacaac
     6961 gtcaatggcg cgattgcctt ctggttcctc gccccgctct gggcacactt tttctcacag
     7021 agataattgg acccagaggc agaaaaaaaa tccacttaga gaatgaatga tgaatcccta
     7081 tcgacgagga caggtgtgct tgggccgact ctggttgacg atcgccgtat gttgcgcggt
     7141 cgtgattttc gcctgtgcct caccggacct cgttttgaca gggtcgtggg gaagcactga
     7201 gatcagacat ccttctcctt ttttttctgg ggcgctggcc tccaacacag aaaggactgt
     7261 catgttgtcc ttgacccagt ctggagaatt catgtggctc gaacctgaag gagtttgcca
     7321 ttcgggaaca tatcttatcc aagccaacgc tctcctttta accgaaacgc aaggaggtga
     7381 acgtattcgc cttgactata ccttccgtgg ggatcaactc cgactgaaaa ccccggatgg
     7441 ttttatattc gagttccgaa agacaccgca ccagactgat gcgggagtta aaccctgtaa
     7501 tcgttagaaa ggaaatacag tgggagccga agaggaggag ttgaaaataa aaattcgtcg
     7561 actggtcaag gagaaatttg aaggtgactt tcgtaaaggc ttcgaccatt acgattccat
     7621 cgatggccag gatttaaaga tcggttcctc cgccttggag caactcctcg ccgatgccaa
     7681 gataggaaat ttcctgaccc gaaccctatg ggtggaaggc gtcttaaagg cactcgacaa
     7741 ggatcaagac ggagccattt catgggatga gttcgcatcg attcttgagg acagcccgca
     7801 aaccaaagaa aaagcagcca gggatccgga tgacgactca tcccctgcca acaaatgagg
     7861 tcgggaatct ttgcttggcc aagctgtggt gcctcaatag aaagcccata atcgttgccg
     7921 ctcacacatt cgtgcgcgcg tcaaacatgt cttcggccat caggtcactg cgatgggcga
     7981 caaattgatt cgaaccctca gcctcgtgtg ggctcggctc cagattgggg tgaggaatct
     8041 caggtataac ttcaaacgat ttctgctcct ctctgctcca tcgaagagac aggtgttctg
     8101 agatagaggc aaccagtgaa gcaaatctct aaaactccat tggggccgct tcatactctc
     8161 tgcttccagg gccatcccaa attttcgaac cgaggccttc gggaattcaa acccacttgg
     8221 tgatttttag aggctccctt ctcctattcg gtatgggttc gattgagggc atgctcctga
     8281 tgagtggcct gatcagtctt ccgtctgtct ttgtttctca atcgtgggat caaaactgaa
     8341 actgagtgcc ggttttctcg caatcggatt tggtaggtat tttttgtgtt ttcccttttc
     8401 ctaagacact tgcgtgcatt cattttagat acgcccacag atgattgtcc cagggagccg
     8461 gatatctgtc attcgttttt cccttcccat gttacccacg ccatttctcg aaaacccatg
     8521 gaccgagaac cttccaaaag aataccggct gagaaaattc ttcattgcga gaacaaaaga
     8581 atttatggtg aaatgacgcc tagcgtggaa cgttgcgttc gtgaaatgaa agagaagata
     8641 cccacttaat cctcagttga caaaatcctc tcttccaaat cctttgtcag aaaaacaaac
     8701 cagcttccag aatttcatta attttcccaa cccacttgga tgacaaagaa aggcatatgc
     8761 tatgagcctg tcacgcatga cgaacatttt cgcaatggtg ctgaccttgg ccatggggat
     8821 catagggacc tcattggccg caaatgagga aatgcacccc ttaccattca tcatcacgga
     8881 catcgaacgc cacattgccg aattgaccat caacattgag aaggtttcgg accggatgaa
     8941 gtttttacgc gaggttccgg attccaagga tcctctcatt caggaagtcc ggaatcttga
     9001 tctgagaggg tgggaactac atcaggaaca atggaagctg caactcgacg gattgcgatt
     9061 cacggaggga ctcttgcgga aagtccagga acatcccgaa gaaaaacccg acgccttaca
     9121 agcctggctt gcccgactga aaggatttaa agaggccatg aacgattatc gacaacagcg
     9181 ggccgggatc gaagtgttgc gcatagaaac agaaagcaag ttgatcgaac aatatttgcg
     9241 ataaggcaag gagacccgaa tggattgcgt aaaacgaccg accgtattat ttgccctatg
     9301 cttgacgggg atatggttca tcatgacagg gtgtacctca gtcgatacca ttttattaac
     9361 cagccaaacc tttccaccca aaaaatcgac tctagatgtg gaagtgatgg ctctagagcc
     9421 aaaatgccct catatcgccc tggcccaatt gagtgtcctg gattcggccg agagttatgg
     9481 agacgaggaa ggggccataa tcaaaaaagc ggctgatttg ggggccgacg ccgttgtgct
     9541 ttataaaggg acaaaacgga cagtcgcgac tcccgcctac ggaggctatg cgtacaacta
     9601 cggctatccc ggatggggtt atgcttctta cggtatgggt ggatacgggg gatacggtac
     9661 gaccggatac acgataggtg gattcggtta ttcgccttac ggcatgggaa cgcccatgat
     9721 gtatgacaca accgtgagat cgctctcggg aattgcaatc cgctaccagg atggcccccg
     9781 ttgcgtacca ggtaagccgg aatccagaca gatccatacc tccttccctc tccaatcata
     9841 aattttcaat ggaatctcta aagttcattt ttagcatccg gctcaacctg accaatgcat
     9901 ccggccttcc ttgaaattaa cattccgtga agttgagaac gaaagaaatc acagaaaaaa
     9961 agaaatgtca cgtatcacct ataagccaga accaacaatg ggttcaagac attgatcgtg
    10021 atgctcgatt tctgctttga cttcagcaac tatgtcattg aaatgttctc cggtttcaga
    10081 aaccatttct gaaacagtgg cgtaggccac aagacctcct tgaataattt ctttgccaag
    10141 ggggcaaaga acggatacta tccctgaggc catcgagggt acagccactg cgcaacagct
    10201 ccgaccacca cacaaccaat gacagcttcc atgcgctcct cctttgtaaa taaaatactt
    10261 cttagaaatc gttaaaagat ttatataacc ggaaagattt agtgaagcat gggggaactc
    10321 aagtaaatca aaaaccaacg cgtcttattc atgccgcgct gtctttcatc ccattgcgca
    10381 aagaaaagag ccatccggtt gtttgaggca atgctgctta agatgaagga gagtcccgag
    10441 agatccctcg ggccatcaag ctccgcacaa gctgaatgca attcatcagg aacgttttgg
    10501 agggaattgt gccaaaactt tctggactgc ctcattgatc aaaccggttt gccctcctgt
    10561 ggttcccttc caatccacca cgcctttggc aaccccacgc cagatgatat gctgaggcgg
    10621 cttcgcatca agcagatcga tgataagcgt cccttcctcg tactggtaaa cttctgtgta
    10681 gcccataccc atcccccagt atggcctcat gagcggacct cgcatggtat cgacctgaat
    10741 ccgctcttcc ctaccaacat aataggtcac atggaaatct gaatttccag cgataatttc
    10801 gatgtacccc ttgttcgcca gttcggctgc gaccgcgtct tgaatccttt gccgggccat
    10861 ttgattggcc ccacccaggt caatgtctcc ttccggcgtt ttcagcatcc aggaaaatgt
    10921 cttcagctca ttaaagtccg tcgactctct ataatcagaa ctgacagaaa tggtggagca
    10981 cccccaaaaa ccgggtaacg ccaacaaaac aaaaagtctc atgaaacatc gcatttgtca
    11041 tatcctttct ttttccagat acatcgcatt cccgaaattc ggattaagtt ttttcatcac
    11101 agagggcact tcctaaagta cgcctgcaga tttcgatttg cctgcacggg agggctgtga
    11161 tcgttcttcc gcttggttga cctccctgaa gattgcctag ttaaaagaga ccaggagcac
    11221 ggcccgttct cccccacaat catgaaggta aattgatagt cgcccaacat actttctgcc
    11281 tgctccggtt tcttagcaca aaactctagc agacacttct cacccgtttc gactggcaac
    11341 tcccagatcc gatgttcctc ctcaagggaa ttccttggat gagcggtggg atggtgattg
    11401 cttcggcatg aatagatacg ttaaaaaccc tggcgtcttt cccgacatgt tcaatcagga
    11461 atttaactgt gtgtgttttc agagaaatac caacaccagg cacgcaggaa tgacgcaagg
    11521 aggatgaata tccgtcgaca attacgaacc cgacaacggc tgttcctgaa atgtatcggc
    11581 acaggagacc ctgcttacat gccgtttttt gtatctcttc ttatcggact tctctttcct
    11641 gcctctcctt cgtttgccga gtggagggtc accggcgaga gtaatgttta ttataccgac
    11701 gatgccgcct tgttctcctc gacacgacgg ctgagtcgaa accaggaccc cacccaaccg
    11761 gttattgata gccttcttgc taaccaggga gacgatgtgg tgtttgaacc catggcccac
    11821 ataatgaaat ctttctcgat gatgggtcga cctaccgaat tgggtgtccg tggacaaggg
    11881 tttgtgttta ccgacaatac ccaattcaat cacggatcat tgagcgtgga ggcctcccat
    11941 aatctgacct ccacctctgc gctcctgatg cgctattttt tcggacccga tttattttta
    12001 ggaaaaaacg aggtacggcc gcttgaggag tccgaaggga atcccccaca agttaaaaat
    12061 gaagaggtca ccaccaatta ttgggctgca gggcttgctc aacaaatccc cggggtcgac
    12121 gatttgcggc ttattctcta tggccgctat ggattgcgtg actacgataa accttttagg
    12181 caacgggata ccagattttg gacgatcggg acgcacgtgg aatgggcacc cacgcagaag
    12241 atcgggttgg ctctgggcta ccattatgaa cgaggattgg ctgatgggcg aaagcagccg
    12301 gagctgcaag atgatacctc ttacttcaac catttcgtga ccggagaatt tgactatgag
    12361 atcacagaac gccttagcct agagatggga cttcattatg aatttaacgg atggaccacc
    12421 ggtatccagg gggatgaacg caagggccaa catgaaaatg tgatccaggg ggacattgcc
    12481 tcccgatacc gggtaaacga gatacttcag atcacagccg ggtttcaggg gtcacatcga
    12541 aaagaaagct ttgaaaaggg cttaaaaaat ctgaacacct ggctgggtgc caaattgact
    12601 ttttaatctt ctcacgccgg aaaaaatgat cctctcagca acctaaggat gggtacatca
    12661 gaaagtttgc ttctttcgag tgttgcatga gtgtgctctg ccttaatgag ttttttgcaa
    12721 gagagtgcta tcttgtgacg cctccacttt gtcaggggtg tcatttcgaa gaaaatttca
    12781 tgctgaagaa gcataacagg tgagagcgag tctctgagca ttgatatttc cacttgccga
    12841 tttcaaacga aaactccaca acccctccag acagggagag ggaacttaag agcgatcagg
    12901 gacggccgat acccaggcta atgtcgaacg aaccaaaaat ttagagcagc ctatagcagg
    12961 gcttcatacc tcagtagagc ttccatgcag aaatggttca ctcaaatcag aaatgccggg
    13021 aaggtcatgc ctctttccat gtgttggttt ctgctgactg catccccgtc cctggcagag
    13081 gaaccccaca aaaagccgac ttctccacca gaagatccct acttgctctg gcgattggat
    13141 gccgccagaa aaaaagtaca agaagtgcct caaaatttaa agggacggat tcggaacagt
    13201 caggagtgga attgggatga atatcccgat caggtgctct tgcaggagga cacggaaatt
    13261 aaatggacac ggtatctgtc tgccgccctc aatgcgccaa aatggttaga tctcggcatg
    13321 accgttcgtc tgcggcggga gggatttgat tttccattta aaacggaaca ggtgggaagc
    13381 acctggcaat gggcgaccag gtcccggttt cgtgccagtg cacggtggaa ggccttccgc
    13441 gttcagctgg aatttcaagg gtccacctcc ggtgtagatt ccgctaccga cgtggtggga
    13501 aacacgacat tcagtgccgg aaacgtgcaa cagttatttg tcgctcacac cttgccgaat
    13561 ttttggcaga ccggacttcg caccgatctt catgtcgggc gcatcaatct ggatattgga
    13621 tcacgtcgcc tggttgcccg gagtcgtttt agcaatacct cccaggcgtt tgacggcatc
    13681 cattggaatc tcgccgatga agatcagtgg caatttcgga cttttttttc tgaagtggtg
    13741 ttagccaccg atactaccaa tcgtctgggg ttgtttacca atgacgggaa ttatttctgg
    13801 ggagtgtcct atgaaaccgc ccagttcgcc tggtcccgaa ttcacatgta ttatttcggc
    13861 aggaaggata atgctggaga taaccaggtg gagcgcgcgc attccacttt tgggttgaga
    13921 ctctatcagt caccacaagt cgggtcgttt gattatgatg
//
LOCUS       DATIAL010000483         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1053448, whole genome shotgun sequence.
ACCESSION   DATIAL010000483 DATIAL010000000
VERSION     DATIAL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053448"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VLA60_14840"
     CDS             <1..289
                     /locus_tag="VLA60_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10684.1"
                     /translation="STIPETKQETSEGATKEPISHLEFPSEPDTETTKSIQESTPDFL
                     KTESIESFESKDLKKLLDSVDASETVTTPPNDPQTPFPTAPPASSSSESSQ"
     gene            501..2027
                     /locus_tag="VLA60_14845"
     CDS             501..2027
                     /locus_tag="VLA60_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HSF10685.1"
                     /translation="MLAKIQSIGLIGLEANLIEIEVDIGGGLPQFSIVGLPDATVRES
                     RDRVRSALKNTGFQFPAKKITVNLAPAGIKKEGAGLELGIAIGILVAEGMLPQEAVTS
                     YIFVGELALDGRIKGVPGALSMAIGTHHPRSLILPQDNASQAAVVDHATVFGVSTLPQ
                     VVEFLRGALTLLPIKANSSTFNPGLPQIEEDFADVAGQYQAKRALEVAAAGGHNLLMV
                     GPPGSGKTMLAQRLTGILPPMSFQECLEASQVHSVAGNLPSHSALLTSRPFRAPHHTI
                     SEAGLVGGGSIPRPGEVSLAHHGVLFLDEALEFKRSLLDSLRQPLENGTVTLTRAQAS
                     LTFPAKLMLVVAMNPCPCGYCGDPTHECVCTPYHIQRYRSRLSGPLLDRLDIHIEVPA
                     VPVKELSGTTNGEASAKIRHRVIQARHRQDERYREEHTLNNAQLKPRLLKKYCQLDQS
                     GKTLLDQAVTRLGLSARAYGRILRVSRTIADLEESDMILPMHVAEAIQYRNLDRPILS
                     "
     gene            2036..2251
                     /locus_tag="VLA60_14850"
     CDS             2036..2251
                     /locus_tag="VLA60_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10686.1"
                     /translation="MESFKVFRWWFVIGALMALAVIMIQGGIRDLMLAKEPIWEIKLI
                     ELGPPIFGGGLLGGCIALILNRIKKKA"
BASE COUNT          632 a          658 c          554 g          596 t
ORIGIN      
        1 gtcaaccata ccggaaacga aacaagaaac ttcggagggg gccacgaaag aaccaatttc
       61 acatttggaa tttccttcag agcctgacac cgaaacaaca aagagcattc aagaatccac
      121 tcccgatttt ctcaagacag aatcgataga gagttttgaa agcaaggatc ttaaaaaact
      181 cttggattcc gtggatgcct ctgagaccgt gacaacccct cccaacgatc cacaaacacc
      241 ttttcccaca gcccctccgg cttcttcttc ctcagaatct tcccaataac aatcctataa
      301 gcctccagat tcgtagaaac tcatcacatc aatcagcccg actagaccag gaatttccaa
      361 acctcatgga atagaagcca ccgcttccac tgacttttcg aatccgatgc ataatgacgt
      421 tgaagcccct acaacctttg cctataatcc atggacccca ttagccaaaa atctcgggag
      481 ggaataatga tgatactggc atgttagcca aaattcagag cataggattg atcggattag
      541 aggccaatct catcgagatt gaggtggata tcggcggggg tctcccgcaa ttttccatcg
      601 tgggactgcc ggatgcaact gttcgcgaaa gccgtgatcg ggtccgatcc gcactgaaaa
      661 ataccggctt tcaatttcca gcaaaaaaaa tcaccgtcaa tttggcgcct gccgggataa
      721 aaaaagaagg ggcaggtctt gagttgggta ttgcgatcgg tattctggtg gctgaaggta
      781 tgttgcctca agaagccgtc acgtcctata tcttcgtcgg tgaactggca ctggacggca
      841 gaatcaaagg cgtccccggg gcactgtcca tggccattgg cacccaccac ccccgttcgc
      901 taattttgcc tcaggacaat gcctcacagg cggccgttgt ggaccacgcc acagtattcg
      961 gtgtctctac cctccctcag gttgtggaat ttcttcgagg ggccctcacg ctccttccta
     1021 ttaaggccaa ttcttcaacc tttaaccccg ggttacccca aatcgaggaa gattttgcag
     1081 atgtggcggg acaatatcag gccaaacgcg ctctggaggt cgccgctgcc gggggacaca
     1141 acctcctcat ggtcggtcct ccgggatcgg ggaaaactat gttagcccaa cggttaacgg
     1201 gcattcttcc gcccatgagt tttcaggaat gcttagaagc cagtcaggtt cacagcgttg
     1261 ccggaaacct tccctctcat tcggcacttc tcacaagccg ccccttccgt gccccccatc
     1321 atacaatttc ggaagccggc ctcgtgggag gcggatccat cccgcgtccc ggagaagtgt
     1381 ccctggcaca tcatggagta ttatttttag atgaagcgtt ggaatttaaa cggtcgttat
     1441 tggatagctt acggcaacca ctggaaaacg ggaccgttac gctcacccgt gcccaggcca
     1501 gtttgacatt tcctgccaaa ctgatgttag tcgttgccat gaatccctgc ccatgcgggt
     1561 attgcggtga ccccactcat gaatgcgtat gtacgcctta tcacattcaa cgctatcgaa
     1621 gccgcctgtc gggtccgctt ctcgaccggc tggatatcca tattgaagtt cctgcagtcc
     1681 cggtgaaaga attatcaggc acgacgaatg gtgaagcatc tgccaaaatt cgtcatcggg
     1741 tgatccaggc cagacaccgt caggatgaac gctaccgtga ggagcacacc ttgaataatg
     1801 cacagcttaa gccccgcttg cttaaaaaat attgccagct tgatcaatca ggaaaaacgc
     1861 ttttggatca agctgtcacc agattggggt tatccgcaag ggcttatgga cgaattttac
     1921 gggtttcacg aactattgcg gatttggaag aatcagatat gattttaccc atgcatgtcg
     1981 ctgaagcaat tcaataccgg aacctggatc ggccgatttt gtcataggac aatcgatgga
     2041 atcctttaaa gtctttcggt ggtggttcgt aatcggagcc ctaatggctc tggccgtcat
     2101 tatgattcaa ggtgggatcc gcgatcttat gctggcgaaa gaacccatat gggaaatcaa
     2161 actcatcgaa ttgggacctc ctatttttgg cggaggactc ttgggagggt gtatcgctct
     2221 catacttaac cgcatcaaaa aaaaggcata gccaaggagc tcctgatccg tcacttacat
     2281 cattctttga actccaacct cgtacccttc tcaagcgcat gctttctcaa gcacatgctt
     2341 tctcaaacac atgctttccc caaacaacgt ctcctctaaa tccagcaaga gtaaaatact
     2401 gtaatcagct tggtcttatt ttactagcat atttcctcta
//
LOCUS       DATIAL010000484        11288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_516263, whole genome shotgun sequence.
ACCESSION   DATIAL010000484 DATIAL010000000
VERSION     DATIAL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516263"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VLA60_14855"
     CDS             complement(<1..266)
                     /locus_tag="VLA60_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSF10687.1"
                     /translation="MDEHTTQGDSCWVNTFVFIAGFLLGAGSAILLTPEAGTHVRDRL
                     ARGAKTAQDEFSDMASQTKEAVHAWSEEARKTMKQAATRVNSAV"
     gene            complement(303..560)
                     /locus_tag="VLA60_14860"
     CDS             complement(303..560)
                     /locus_tag="VLA60_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10688.1"
                     /translation="MTMSKLNTAHASDQESYANQSTPEGGLGWEGHVHFSEDVPLWKR
                     VLDTWPELRTGLSYSLIFASGVLLGGAIMAMASAKNHEDRS"
     gene            complement(557..3463)
                     /gene="treY"
                     /locus_tag="VLA60_14865"
     CDS             complement(557..3463)
                     /gene="treY"
                     /locus_tag="VLA60_14865"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HSF10689.1"
                     /translation="MTPLPLHIPLSTYRLQFNASFTFQDASGILPYLSQLGITDCYAS
                     PYLKATPGSTHGYDVVDPTELNPEIGTEADYQVFIQALHEHGMGQILDVVPNHMGIAG
                     STNLWWQDVLENGPSSHYATFFDIDWTPVKPELENKVLLPILGDQYGIVLENQEIILH
                     YDDGQFFLTYYENRLPIDPCTWSTILTFRQDTLNQSLENSDPHLHEYQSIITALSHLP
                     ARTETEAERVAERYREKEVIRRRLSTVIADNSTIRDFLLENIRLLNGMKNSPRTFDVL
                     DRLVSHQAYRLAYWRVAAEEINYRRFFDINQLAAIRMEQPEVFQSAHQKIFELLTSGA
                     VNGLRIDHVDGLYNPRAFLAQWQQWAAEHLEVQADTQGRSLYLLVEKILGTEERLTSD
                     WLCHGTTGYDYLALVNQLFIQETHQRQIEQVYSRFIKQSLHYDDLIYDCKNLIMTSSM
                     SGEINALGHQLNVLSERNRRSRDFTLNSLIHAIREIIACFPVYRTYIGPDPLEGVLDR
                     DRAYIRLAVARAKRRNPAISNLVFDFIRDLLLRIPQNSPDLDWEVIRPFVMKFQQTTS
                     PVTAKGVEDTAFYCFNRLTSLNEVGGEPQHFGIPLSTFHQYMQDRATQWPASLSSTST
                     HDTKRSEDVRARINVLSEIPKEWRQHLKTWNRLNKKAKQTINDQRAPSLNEEYLIYQT
                     LLGTWPLAGLSEPAQPQFLGRMQGYMVKALREAKVNTSWLNPDEAWENAVGEFLSRIL
                     SLRPSNVFLQDFIPFQQRISKYGIYNSLSQVLIKILAPGVPDFYQGTELWDFSLVDPD
                     NRQSVDYLFRQQRLSELQHMQQTTAPLDLVQTLLQDAESGLIKMYLTTTALHIRKSHP
                     HLFLEGSYLPLEFKGEQAHHACGFIRQHHSHMCLVIFPRLLTTLIPDQTISPLGESIW
                     GQTSMRLPPEFAAHSFRNLLTQEIVTPQNGPSMVGLSLGMLFQHFPFALLEPVS"
     gene            complement(3474..4766)
                     /gene="glgC"
                     /locus_tag="VLA60_14870"
     CDS             complement(3474..4766)
                     /gene="glgC"
                     /locus_tag="VLA60_14870"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HSF10690.1"
                     /translation="MKSPSGPRRKKIIERTPQKILAMIMAGGKGERLMPLTEQRSKPS
                     VPFAGTYRIVDFVLSNFINSGIQSMYVLVQYRSQSLIEHLRRAWRFGGTNRQNFITVV
                     PPQMKGRGKWYEGTADAVYQNINLIQDFAPGLVAVFGADHIYRMDVRQMIQFHLDQEA
                     DVTVASLPVPIQAAKGFGIVEVNADHQIIGFEEKPASPKPMPENPAMAFSSMGNYIFN
                     ADILVKILEQDASQAGTHDFGRNIIPSLIKSHRVLAYDFMDNVVPGIHPYEEWGYWRD
                     VGTLEAYWQANMDILGESPILDLRNVKWPILTDASVEPVASLVRSHMDDAMVGQGSLV
                     VDSKVKRSIIGRNVRIGEGCTIEESIILDGTLIGSNCHLHRCIIDRFNIISSGTTLGD
                     KHGRDSRRSTAGKLGLTLFPRGQSYGGRAIHSSPSSLT"
     gene            complement(4773..5054)
                     /locus_tag="VLA60_14875"
     CDS             complement(4773..5054)
                     /locus_tag="VLA60_14875"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HSF10691.1"
                     /translation="MYSALLALLLLLFFAVAFSIQNSEPIILNFFAWTFQGSRALLLL
                     TALALGVGLTVLAALPLYIKKIRQIAKQQKTIATLERSVAEFTNPPSNT"
     gene            5111..7315
                     /gene="glgB"
                     /locus_tag="VLA60_14880"
     CDS             5111..7315
                     /gene="glgB"
                     /locus_tag="VLA60_14880"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HSF10692.1"
                     /translation="MPTLTNRFQRLVSATEWDPFSVLGPHRGESTDSSGVCIRAFLPE
                     AAEVAVLPGQNGNTPIPMKLVHPAGVFEARWEGDPLGTDYQFRIVDRQGKFSQRHDPY
                     AFLPRISDFDLHLFGEGKLYRAYEQLGGQVCIHQGVQGVNFAVWAPNAKRVSVVGDFN
                     EWDGRRHPMRSRGGGGIWELFIPDMNEGAVYKFEILPQNGDAPFLKADPYASSAELRP
                     KTASVVRDLSGYVWRDGEWMAARQNRDPLAQPWSIYEVHLGSWRRVPEEGSRWLTYQE
                     LAETLIPYVKDMGFTHIELMPVTEHPFDGSWGYQATGYFAPTSRFGTPAQFMAFVDAC
                     HQAGIGVLMDWAPAHFPEDPHGLAWFDGTNLYDHSDPRLGFHPEWNSRIFNYGRTEVK
                     NFLINSALSWFDRYHIDGLRVDAVASMLYLDYARKSGEWIPNKFGGRENLEAVEFLKE
                     LNTVAHQEHPGIVMIAEESTAWPGVSKPTYVGGLGFTFKWNMGWMHDTLDYFSLDPIY
                     RRYHQHNVTFGLVYAFTENFVLPLSHDEVVHGKKSLLDKMPGDEWQRFANLRALYGHM
                     WGHPGKKMLFMGCEIGQWWEWNHDDSLQWHLLEYDRHQGLQRYVADLNRLYASQPALH
                     QVDYDWTGFQWIDLHDSDHSTLTYFRRAKDPSDIVVCALNLTPVPREAYRMGVPTAGY
                     YRELLNSDSEVYGGSNMGNAGGVQAEDMPWHGQPFSVVITLPPLAAVFFKPA"
     gene            complement(7657..9534)
                     /gene="treZ"
                     /locus_tag="VLA60_14885"
     CDS             complement(7657..9534)
                     /gene="treZ"
                     /locus_tag="VLA60_14885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HSF10693.1"
                     /translation="MEKLKAVGWQLELGASCIDPSSVEFRVWAPKAQSVAVKIMGSTG
                     IPTPLSQESFGYWKGTVQDLSPGTRYQYVLNHNMERPDPASRSQPDGVHGPSEIINPL
                     AFSWTDTAWRGLPLDQYIIYELHTGTFTQDGTFDAIISRLPYLRDQIGITAIELLPVA
                     QCPGFRNWGYDGTFLFAPQHNYGGPDGLKRLVNACHAAGLAVIMDVVYNHLGPEGNYL
                     GDFGPYFTDTYKTPWGSGLNYDGPDSDAVRHFMISNAVYWIHEYHIDALRLDAIHGIF
                     DFSAKHLLQDIGEAVHVEGERLERQVQVIAESDLNDVRIISPPSRGGYGLDGQWSDDF
                     HHALHALLTKERKGYYEDFGQLKDLVKAIREGVVYSGQYSPHRRRKHGNSFKPCSPTQ
                     LVVCAQNHDQIGNRAQGDRLSSLVSFEALKVANATVLLSPYLPLLFMGEEYGETAPFL
                     YFIDHGDPALIEAVRQGRTREFAAFGWTDIPDPYAQETFVRSTIHPNLETNPQQHAHA
                     RWCHALIHLRKSIPALGTGAKGHRIQIGSHAKSQLLIIHRRAKQGPEALILLGFHHKA
                     SLALVKLPKGNWESRLDSGTFVAGDQEELLAPSHLAVQNVEQVSLKLPPYPAWIFLKS
                     D"
     gene            9741..>11288
                     /gene="glgX"
                     /locus_tag="VLA60_14890"
     CDS             9741..>11288
                     /gene="glgX"
                     /locus_tag="VLA60_14890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HSF10694.1"
                     /translation="MRIWPGRSYPLGATWDGQGVNFALFSENATGVDLCLFDNELQDQ
                     ETHRITIEERTDQVWHVYLPEVRPGQLYGYRVHGPYDPEAGHRFNPAKLLIDPYAKSL
                     TGVVRWSDSMFGYRLGDPAGDLSFDDRNNAANIPKGVVVDQAFSWGGDQRLNIPWEQT
                     VIYEVHVKGMTMRHPDVPESLRGTYAGLASPAILEYLQSLGITAVELLPVHHFVNDLY
                     LKEKGLTNYWGYNSIGYFAPDIRYSAFPGGGEKVDEFKSMVRKLHSAGIEVILDVVYN
                     HTGEGNHFGPTLSFRGIDNASYYRVSPDNPRYYMDYTGCGNTLNVRHPRTLQLIMDSL
                     RYWVNDMHVDGFRFDLASALARELHDVDRLSAFFDIIHQDPVLSQVKLIAEPWDLGEG
                     GYQVGNFPPGWAEWNGKYRDSIRRYWKGDGGLLGEIANRWSGSSDLYGESGRQPYASI
                     NFVTAHDGFTLQDLVSYDHKHNENNQEGNRDGTDDNDSWNCGVEGPTDDPAIQELRDR
                     QVRNFFAT"
BASE COUNT         2681 a         2621 c         3127 g         2859 t
ORIGIN      
        1 tccacagcgg agttgactcg agtggccgcc tgtttcatgg tttttctggc ctcttcagac
       61 caggcatgca cggcctcttt tgtttgagaa gccatatctg aaaattcgtc ctgtgcggtc
      121 ttcgcacctc gtgccaatcg atcacgcacg tgagtccctg cctcaggcgt caggagtatg
      181 gcgctaccgg cgcccaatag aaatcctgca atgaagacaa atgtattaac ccaacatgag
      241 tcgccttgcg tcgtatgctc atccatcgtg aagttcctcc ttagtaatgc tttgtttgat
      301 tgttatgacc ggtcctcatg gttcttcgct gaggccattg ccatgatggc tcctcccagg
      361 agaactccag aggcaaagat aagactgtag gataagcccg ttcggagttc aggccatgtg
      421 tccagaaccc gtttccatag tgggacatct tcggaaaaat ggacatgtcc ctcccacccc
      481 aatcctccct ccggcgtgga ctgattagcg tatgattctt ggtccgaagc gtgtgccgtg
      541 ttcaatttgc tcatagtcat gagacgggct ccaatagggc aaaaggaaaa tgttgaaaaa
      601 gcattcctaa ggataatcct accatgctcg ggccattttg tggagtgaca atttcctggg
      661 ttaagagatt acgaaacgag tgtgccgcga attccggagg gagccgcatc gaggtttgtc
      721 cccaaatgga ttcgccgagc ggactgatgg tctggtcggg gatgagggtc gttaacaggc
      781 gtgggaatat gaccaagcac atatgtgagt gatgttgtct tatgaatccg cacgcatgat
      841 gggcctgttc tcccttgaat tccaggggta aatacgatcc ttccagaaac agatgggggt
      901 ggctcttccg gatatgaagc gcggtagtgg ttaaatacat tttgattagg ccgctttccg
      961 catcctgtaa taacgtctgg acaaggtcga gaggagcagt cgtctgttgc atgtgttgta
     1021 attcggacag ccgttgttgt ctgaagaggt aatccacgga ttgtcggttg tctgggtcga
     1081 ccagactgaa atcccaaagc tccgtgcctt gataaaaatc ggggacaccg ggagcaagga
     1141 ttttaataag gacctgactc agagaattat agatgccata tttggaaatg cgttgttgaa
     1201 aggggatgaa atcttgaaga aagacattgg aaggcctgag agaaagaatg cgggaaagaa
     1261 attccccgac ggcgttttcc caggcttcat cgggatttaa ccagctggtg ttcactttcg
     1321 cttcccgtaa ggctttaacc atatacccct gcatgcgccc caagaattga ggttgagccg
     1381 gttccgaaag accagccaat ggccaggtgc cgagcagcgt ctggtaaatc aaatattctt
     1441 cattcaagct cggtgcccgt tgatcattga ttgtttgctt ggcttttttg ttcagccgat
     1501 tccaggtttt cagatgctgc cgccattctt tgggaatttc cgacaacaca ttgattcggg
     1561 cccggacatc ctcgctgcgt ttcgtgtcat gggtggacgt ggaggagagg cttgctggcc
     1621 attgggtggc ccgatcctgc atgtattggt gaaatgttga aagcgggatc ccaaagtgtt
     1681 gcggctcgcc gccgacttca ttgagagatg tgaggcggtt gaaacaatag aaggcggtat
     1741 cttcaacgcc tttcgcggtg accgggctgg tcgtttgctg gaatttcatg acaaaagggc
     1801 gaatcacctc ccaatcaaga tccggtgaat tctggggaat tctcaagagg agatcgcgga
     1861 taaaatcgaa gaccagattg ctgatggcgg gattgcgtcg tttagctcgg gccactgcta
     1921 gccgaatata cgcgcgatca cgatccagga cgccttcgag aggatcgggt ccaatatatg
     1981 ttcggtacac cgggaaacag gcaatgattt cgcggatcgc atggatcaga ctattgagcg
     2041 taaagtcccg tgagcgccgg tttcgctcgg agaggacatt cagctgatgc cccaaggcat
     2101 tgatttctcc tgacatggaa ctggtcatga tgaggttttt gcagtcgtag atcagatcgt
     2161 catagtggag tgactgtttg ataaatcggc tgtagacctg ttcgatctgt cgttggtggg
     2221 tttcctggat gaataattga ttgacgaggg caaggtagtc atatccagtt gtgccatggc
     2281 acagccaatc ggaagtgaga cgttcctctg tacccaggat tttttcaacg aggagataga
     2341 gtgagcggcc ttgggtgtcg gcctgaacct ccaagtgttc agccgcccat tgttgccatt
     2401 gggccagaaa ggctctggga ttataaagcc cgtcgacatg atcaattcgc agtccattca
     2461 cagcaccgga tgttaataat tcaaatattt tttgatgggc cgattggaag acctccggtt
     2521 gctccatgcg aatggccgcc aattggttga tatcaaaaaa ccgtcgataa tttatttctt
     2581 cagcggcgac ccgccaatag gccaggcgat aggcttggtg actcaccagg cggtcgagga
     2641 catcgaatgt gcggggagag ttcttcatgc cattaagtaa ccgaatgttc tccagcagaa
     2701 aatcgcgaat agttgaattg tcggcaatca ccgtggagag gcggcggcgg atcacctctt
     2761 tctcccggta tcgttcggca acccgctcgg cttccgtctc ggttctggcc ggtaaatggg
     2821 aaagagcggt gataatgctt tggtattcat gcaagtgcgg atcagaattt tctaggcttt
     2881 ggttgagggt gtcttgcctg aaggtcagaa tggtactcca tgtgcaggga tcaataggca
     2941 gacgattttc atagtaggtg aggaaaaatt gcccgtcgtc ataatggagg atgatttcct
     3001 ggttttcgag aacaatgccg tattgatcgc cgagaatggg aagaagcacc ttgttttcta
     3061 attccggctt aaccggtgtc cagtcaatat cgaaaaatgt ggcgtaatgt gaggaaggac
     3121 cattttccaa gacatcctgc caccaaagat tcgtcgatcc ggctatcccc atatgattcg
     3181 ggacgacgtc cagtatctga cccatgccat gttcgtggag tgcctggata aatacctgat
     3241 aatcggcttc cgtcccgatc tccggattta attccgtggg atcgaccacg tcgtatccat
     3301 gagtgctccc tggagtggct ttcaggtagg gagaggcgta acagtccgta atgccgagtt
     3361 gagacagata cgggagaatg ccagaggcgt cctggaatgt gaatgacgcg ttaaattgta
     3421 ggcgataggt cgagagcgga atgtgcagag gcagaggagt catgctccct ccttcatgtt
     3481 aaagaggagg gtgaggaatg gatggcccgt ccgccatacg attgcccacg agggaagaga
     3541 gtgaggccga gcttcccggc tgtggatcgt cgactgtctc gtccatgttt gtcccctagt
     3601 gtggttccgg acgagataat gttaaatcgg tcaatgatac aacgatgaag atggcaattg
     3661 gatccaatga gtgtcccatc aagaataatc gattcttcaa tggtacagcc ttctccaatt
     3721 ctgacattgc gtccaatgat ggaacgtttc accttactgt ctacgacgag gctgccttgg
     3781 ccgaccatgg catcatccat gtgggatcgg actagactgg caacaggttc aaccgacgca
     3841 tcggtcagaa taggccattt gacatttcgt aagtcaagga tcggcgactc ccccaaaata
     3901 tccatgttcg cctgccaata ggcctccagg gttcccacat cgcgccagta cccccattct
     3961 tcgtaaggat gaatgccggg aaccacattg tccataaagt cataagcgag gacccggtga
     4021 gatttgatga gagacggaat gatgttccgg ccaaaatcgt gcgtgccagc ttgactggca
     4081 tcttgttcca gaattttcac aagaatgtcg gcgttaaaaa tataatttcc catcgaacta
     4141 aacgccatgg cgggattttc cggcatgggt ttgggagagg ccggcttttc ctcaaatccg
     4201 ataatttgat ggtcggcatt gacttcgaca attccgaacc cctttgccgc ctgaatcgga
     4261 acgggaagcg acgcgacggt cacatccgct tcctggtcaa gatgaaattg gatcatctgc
     4321 ctcacatcca tccggtaaat atgatcggct ccgaagacgg ccaccaatcc tggcgcaaag
     4381 tcctggatga ggttaatatt ctgatagacg gcatctgctg tcccttcata ccatttgccg
     4441 cgtcccttca tctggggagg aacgaccgta ataaagtttt gacggttggt gccaccaaaa
     4501 cgccaggccc ttctcaggtg ttcaatcaag gattgtgaac ggtattgcac cagcacatac
     4561 atggactgga tgccggaatt aataaaattg ctgagaacaa aatcgacaat acgataggtg
     4621 ccggcaaacg gcacggacgg cttacttcgt tgttccgtta acggcatgag ccgttcgcct
     4681 ttgccaccgg ccatgatcat ggccaggatt ttttggggcg tccgttcgat gatttttttg
     4741 cggcgaggtc cggaagggct tttcatacag atttaggtgt tggacggagg attggtgaac
     4801 tcggcgacgg atcgttccag tgtcgcgatc gttttttgtt gcttggcgat ctggcggatt
     4861 tttttgatat agaggggcaa tgcagcaagt acggtcaggc cgacacctaa tgccaaagct
     4921 gtgagcaaca acagagcccg cgacccttga aaggtccagg cgaaaaagtt cagaataatg
     4981 ggttcggagt tttgtatcga aaaagcaacc gcaaagaaca acagaagcag caaggccaac
     5041 agtgcagaat acatagtccc ttttgttatg ttaggatatt gttaattaac acagaaagga
     5101 ttcattggca atgccaaccc tcacaaatcg ttttcaacgt ctggtttccg ccactgaatg
     5161 ggatccgttt tccgtgttgg gtccccatcg tggggaatcg acggattcgt ctggagtctg
     5221 catcagggct ttcctgccag aggctgccga agttgcagtg cttcctggtc aaaatggcaa
     5281 cacccctatt cccatgaaac tcgtgcatcc tgccggagtc tttgaagctc gatgggaggg
     5341 ggatccattg ggcacggatt accagtttcg cattgttgat cggcagggaa aattcagtca
     5401 acgccatgac ccttatgcgt ttcttccgcg catctcagat tttgatttac atttgttcgg
     5461 tgagggcaag ctttatagag cctatgaaca gcttgggggg caagtatgca tccatcaggg
     5521 tgttcaaggg gtcaattttg ctgtgtgggc acccaatgcc aaacgggtca gcgtggtagg
     5581 agacttcaat gaatgggatg ggcgtcggca tccgatgaga agccgcggag gtggtggaat
     5641 ttgggagttg ttcattccgg atatgaatga gggcgcggtg tataaattcg aaattttacc
     5701 gcaaaatggg gatgcgccgt ttttgaaagc ggacccgtat gcttcctcag ctgaattacg
     5761 accgaaaacg gcctctgtcg tccgtgatct ttcgggttat gtatggcggg atggagagtg
     5821 gatggcagcc aggcaaaatc gtgatcccct cgctcaacct tggtccattt atgaggtgca
     5881 tttgggatcc tggagacgtg ttccggagga agggtctcga tggttgacct accaggaatt
     5941 agcggaaacc ctcattccct atgtgaagga catggggttt acccacattg agctgatgcc
     6001 ggtgaccgaa catccctttg acgggtcatg ggggtatcag gccaccggat attttgcacc
     6061 cacaagccgg tttggcactc ccgcgcagtt tatggcgttt gtggatgcct gtcatcaggc
     6121 cggaatcggg gtcctgatgg attgggctcc tgcgcacttt ccggaagatc cccatggttt
     6181 ggcctggttt gacgggacaa atttgtatga ccattcggac ccgcggctcg gattccatcc
     6241 agaatggaat agccgaattt ttaattacgg tcggaccgaa gttaaaaatt ttctgatcaa
     6301 tagcgcactc agctggtttg accggtatca tattgatggc cttcgggtag atgctgtcgc
     6361 atcgatgctc tatctcgatt atgccagaaa atccggtgaa tggattccaa ataaattcgg
     6421 gggtagagag aatcttgaag cagtggaatt tttaaaagag ctcaataccg tggctcatca
     6481 ggaacatccc ggcattgtga tgattgccga agaatccaca gcatggcctg gggtctcaaa
     6541 gccgacttat gtcggagggt tgggatttac ctttaaatgg aatatggggt ggatgcacga
     6601 taccctggat tattttagtc tggatcctat ttatcgaaga tatcatcaac ataacgtgac
     6661 cttcggatta gtctatgctt tcaccgaaaa cttcgtcctc ccgctttctc atgatgaagt
     6721 ggtccatggc aagaaatcgc ttctggataa aatgccgggg gacgaatggc aacgatttgc
     6781 gaatttgcgg gccttgtatg gacacatgtg gggtcatcct gggaagaaaa tgttattcat
     6841 gggctgcgag attggccaat ggtgggaatg gaaccacgat gatagcctgc aatggcatct
     6901 acttgagtat gatcggcatc agggtctcca gcgttatgtc gctgatctca atcgattata
     6961 tgcctcacaa cccgcgctcc atcaagttga ctatgattgg acggggtttc agtggatcga
     7021 tcttcatgac agtgatcatt cgacgctcac gtatttcagg cgcgccaaag atccttccga
     7081 tattgtggtc tgtgccctga acctcacacc tgttcctcga gaagcctatc gaatgggcgt
     7141 gccgaccgcc gggtattatc gagagctttt gaacagtgac tctgaggtgt acggtggaag
     7201 caatatgggg aatgctggag gagtacaggc cgaagacatg ccttggcatg gccagccgtt
     7261 ttctgtggtt attacccttc cgcctttagc cgcagttttt tttaagcctg cgtagattcc
     7321 caagatctcc gtgatatgtc taagcttcct cctccctttc gatcaggtta agaggggagc
     7381 agacaaggac ggttatcacc ccggaaagag aagccgcgat cttcacccat atgggagtgt
     7441 tgaataataa agattttcaa gggccaattt gcccaagatt agattactca tcaaaggctc
     7501 cgggtcggtt caaaaaagtc agtccagcaa ggccgcagcc gttttttcgc gcggagcgta
     7561 cgcttagtac gtgagcacgg aaaaatggcg agaacgccgc tggcggcttt tttcaacagg
     7621 ccccatatgc ccggccttca tggtcttggc ctgaacctag tcagatttca gaaaaatcca
     7681 ggcgggatag ggtggcagct tgagagagac ctgctccaca ttctgaacgg ctagatggga
     7741 gggagccagg agttcttcct gatccccagc gacgaaggtt ccactatcca ggcgggattc
     7801 ccaattccct tttggaagct tcactaacgc taaagaggcc ttgtggtgaa accccaggag
     7861 gatcagggct tcggggccct gtttggcccg gcgatgaata atcagcaatt gacttttcgc
     7921 atgtgacccg atctggattc tgtggccttt cgctccggtg cctaatgcgg gaatagattt
     7981 gcgcagatga atcaacgcat ggcaccaccg cgcgtgtgcg tgttgttggg gattggtctc
     8041 aagattagga tgaatggtgg aacgaacgaa ggtctcttga gcatagggat caggaatgtc
     8101 cgtccatccg aacgccgcga attccctggt tctcccctgt cggacggctt caattaacgc
     8161 aggatccccg tggtcgatga aatacaaaaa aggtgcggtt tcgccgtatt cttcccccat
     8221 aaataagaga ggaagatacg gggagagaag cacggtggca ttggcgactt tgagggcttc
     8281 aaagctcacc aagctgctga gtcggtctcc ctgtgcccga ttgccgattt gatcatgatt
     8341 ttgggcgcat accacaagtt gtgtgggtga gcagggcttg aaggaattgc catgtttccg
     8401 tcgccgatgt ggagaatatt gtcctgaata gaccacgcct tcccgtatgg ccttcaccag
     8461 gtctttgagt tgtccaaagt cttcatagta gcccttacgc tctttggtga gcagggcatg
     8521 tagggcatga tgaaagtcat cgctccattg cccgtccagc ccatatcccc ctcgtgaggg
     8581 aggcgaaatg atgcggacat cattcagatc gctttcggca atgacctgca cctggcgctc
     8641 gagtcgttct ccctccacat ggacggcttc cccaatgtcc tgaagaagat gtttggcgct
     8701 gaagtcgaag atgccgtgaa tcgcatctaa gcgtaacgca tcaatgtggt attcatgaat
     8761 ccaatagacc gcattgctga tcataaaatg tctgacagca tcactatcgg gtccatcgta
     8821 gttgaggccg ctcccccatg gggttttata ggtgtcagta aaatatgggc caaaatcacc
     8881 aaggtaattg ccttccggac ccaggtggtt atagaccacg tccatgatga ccgcaagacc
     8941 ggcagcatga caggcattga ccaaccgctt gaggccatcg gggccaccat agttgtgttg
     9001 gggagcaaag aggaaggttc catcgtatcc ccagtttctg aacccagggc attgcgccac
     9061 cggtaagagt tcaatagccg tgatgccaat ctggtcgcgc aaatagggca gacggctgat
     9121 gatggcatca aaggtcccgt cctgtgtaaa tgtgccggta tggagctcgt agatgatgta
     9181 ttggtcgagg ggcaggccac gccaagctgt atccgtccag gaaaatgcca gaggattgat
     9241 aatttccgag ggaccgtgaa cgccgtccgg ttgtgatcgg gaagccggat cagggcgttc
     9301 catgttgtga tttaatacat attgatagcg ggtgcctgga gaaaggtcct gcacggtgcc
     9361 tttccaatat ccaaaggatt cctggctgag gggagtcggt attccggtac ttcccatgat
     9421 tttgacggcc accgactggg ccttgggggc ccacacccga aactcgacag aagaggggtc
     9481 gatacacgat gcgcccagtt ctaattgcca tccaacggct tttagttttt ccaaacgtat
     9541 ttcctccctg ctggtttttt tttgatcaga ttgttagctg gtgggcaatg ggataaagga
     9601 aagcttaaca aaaagacaga gagctgtata tggaatgcca gaagcatctg actaggtttg
     9661 ccccgttttg agatatactg tattatcgtc ccccgctatt cccgttatcc ggaaacccac
     9721 atccaggaga gcagaataat atgagaattt ggcctggtcg atcatatccg cttggtgcca
     9781 catgggatgg gcagggagtc aattttgcgc tgttttccga aaatgcaacc ggtgtggatc
     9841 tttgtctgtt tgacaatgaa cttcaggacc aggaaaccca ccggataacc attgaggaac
     9901 gaacggatca agtctggcat gtgtatttac ctgaggtgcg tcccggccaa ctctatggat
     9961 accgggtgca tgggccatat gatcctgagg cggggcaccg gtttaaccct gccaagcttc
    10021 tcatcgatcc gtatgcgaaa tccttgactg gtgtcgtgcg atggtccgat tccatgtttg
    10081 gctaccggct tggcgatccg gcaggtgacc tttcattcga tgatcggaac aatgcggcaa
    10141 acattcctaa aggggtggtc gtcgatcagg cctttagctg gggaggagac caacgtctga
    10201 atattccctg ggagcagacg gtcatctatg aagtccacgt caagggaatg acgatgcgac
    10261 atccggacgt tcctgaatca ttgaggggaa cctatgcagg cttggcgtct ccggcgattc
    10321 ttgaatattt gcaatcgctc gggatcaccg cagtggaatt gttgccggtt caccattttg
    10381 tgaatgatct ctatctgaaa gaaaagggtc tcacgaatta ttgggggtat aactccatcg
    10441 ggtattttgc cccggacatc cggtattcgg ccttccccgg tggaggggaa aaggtcgatg
    10501 agttcaaatc catggtcagg aaacttcata gtgccggcat cgaagtcatc cttgatgtgg
    10561 tctataacca taccggagaa gggaatcact tcgggccgac gttatcgttc cgggggattg
    10621 ataacgcctc ctattatcgg gtgtctccgg acaatccacg ttattacatg gattacaccg
    10681 gctgtggaaa tacgttgaat gtccgacatc cacggacctt acagttgatc atggacagtt
    10741 tgcggtattg ggtgaatgat atgcatgtgg atggtttccg gtttgatctg gcatccgcgc
    10801 tcgcacggga attacatgat gtggatcgac tcagcgcctt ttttgacatt attcaccagg
    10861 atcccgtgtt gtcccaggtg aagctgattg ccgaaccatg ggatctgggg gaaggcggct
    10921 atcaagtggg gaattttcct cctgggtggg cggagtggaa tgggaaatac cgcgattcca
    10981 tccgccggta ttggaaggga gatggcgggt tgcttgggga aatcgccaat cgttggtccg
    11041 ggagcagcga tctctacgga gaaagcgggc gacaacccta tgccagtata aattttgtga
    11101 ccgcgcatga cggatttacc ctccaggatt tggtgtcgta tgaccacaag cataatgaaa
    11161 acaatcagga aggaaatcgg gatggaaccg atgacaatga tagttggaat tgtggtgtgg
    11221 aaggcccgac ggatgatcct gcgattcagg aattgcgtga tcggcaggtc cggaattttt
    11281 tcgcgact
//
LOCUS       DATIAL010000485         5594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1485385, whole genome shotgun sequence.
ACCESSION   DATIAL010000485 DATIAL010000000
VERSION     DATIAL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485385"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VLA60_14895"
     CDS             <1..1430
                     /locus_tag="VLA60_14895"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSF10695.1"
                     /translation="FTIFGAGRAIPLLDTNSMLINFLGPPGGPARKGPFPILPFVDVL
                     LGKFDKSLVKNKIVLIGLTVRGLEEFSTPTTSNTKMWGVEVLGNAIETILNQRYVVPA
                     SRTLTVVLIFLMAALAAWLVALSRPSWATIGSLILLALYVFIAGVFFDAGILFNLIYP
                     PSALLISFSLVMAYRVVDEQGEQRKIRELMGQYLSPTVSQWVLQDPDKLQLGGQTQDV
                     TVLFSDIRGFTTLAHTLAPQPLVSLLNEYMTVMSRIMFKHDGVVDKYIGDAIMAFWNA
                     PMPQPDHARRACQTALEMITTLRELQADWKRRGMPMLEVGIGINSGPAVVGNIGSIER
                     LQYTALGDTTNVASRLEALSVVYKVSIVIGEFTRQEAGPAFEYRELDLVALKGRDEPL
                     VVYEVVGLAGQLNHDQAQVLERYRCGLDLYRSRRWSEAQAVFRDIQVKAPDDGPTALY
                     LHRSTKFCANPPPADWNGVYVAKTK"
     gene            complement(1378..2589)
                     /locus_tag="VLA60_14900"
     CDS             complement(1378..2589)
                     /locus_tag="VLA60_14900"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HSF10696.1"
                     /translation="MIPLDQSNPAMNFRNFHLEGWLIFRRFSLVKTLFRGFVGFLVLY
                     GPGSVQATEPTMVTATATLSVLVGPVSLIPAGNTESNAAKDGMSLNVSDRIVTGTQGM
                     ALVTFLDGSTLTVQPDSDVEVKKAEVSDEGSRISIWIHLGTVWARVVSFVDPDSSFSL
                     ESNTATATVHDGLIGGQQNPDTSFICWTLAGDLLVEDRMGQSLVTLKSGEKTKVQDGQ
                     DSGRHAFAVHHSTLKVTASPNLLPLVLMPDNVRVSGFVAPGLEVNQVFGSHTGIEGYG
                     TRTVEVPAGVPGPFTLIVEGKAETTFTVMVEGFFKGTEVYRQNFAGRITKGERLSTRI
                     TQELESTPVSNPKTAKVLNGQAEELRSLQESLPGIILVSPRESKQLTELAEGAIWSLP
                     HTRHSNRLEAD"
     gene            2989..3432
                     /gene="tnpA"
                     /locus_tag="VLA60_14905"
     CDS             2989..3432
                     /gene="tnpA"
                     /locus_tag="VLA60_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044282811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HSF10697.1"
                     /translation="MSEYIHKNHNGTVLLYHLVFPAKYRRAIFDATVDKVLKDVCLEI
                     EQRYQVKFLEIGTDKHHVHFLVQAVPGYSVTKVGTLIKSLSAKEIFRRVPQVKEQLWG
                     GELWTDGYDASTVGKHGNETMIGKYVKQQGEAYQKLYTNHQLVLF"
     gene            5242..>5594
                     /locus_tag="VLA60_14910"
     CDS             5242..>5594
                     /locus_tag="VLA60_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012640573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HSF10698.1"
                     /translation="MFLVHGIDHEGQAVLRKKLRRGHVLKFFGTLPPCLVGMEGCGSA
                     HYWARALSAQGHQVRLIPPQSVRPFLKTNKHDAADAEALAEALVRPTMRFAPPKSADQ
                     QAVLMLHRSRELLVK"
BASE COUNT         1417 a         1396 c         1383 g         1398 t
ORIGIN      
        1 acttcaccat tttcggagcg ggacgtgcca taccccttct ggacacaaac agtatgttga
       61 tcaatttttt gggtccccca ggagggcctg cgcgaaaagg cccctttcca atcctcccct
      121 ttgttgatgt cctgcttggg aaattcgaca aatctttggt gaaaaacaaa attgtcctca
      181 tcggtctcac tgtgcgtgga ctcgaagagt tttctacgcc aaccacgtca aacaccaaaa
      241 tgtggggtgt agaagtcctg gggaatgcga ttgagaccat tctgaatcaa cggtatgtag
      301 tgccagcttc tcgaaccctg accgttgtgt tgattttcct aatggctgca ttggccgcgt
      361 ggcttgtggc cctatcccgc ccttcttggg cgacaatcgg gagcctgata ctccttgcgc
      421 tctatgtatt catcgcagga gtcttctttg atgcgggaat actttttaat ttgatctatc
      481 cacccagtgc gttattgatc tcattcagtt tggtcatggc ttaccgtgtg gtcgatgagc
      541 agggggagca acgaaaaata cgggagctga tgggacaata cctttctccg actgtgagtc
      601 agtgggttct gcaagacccc gacaagttac agttaggcgg ccaaactcag gatgtcacgg
      661 tgctcttttc tgatattcga gggttcacga cgttggcaca taccctcgct ccccaaccgc
      721 tggtctcgct cctcaacgaa tatatgaccg tgatgagtcg catcatgttc aaacacgacg
      781 gagtcgtaga caaatacatt ggcgatgcca ttatggcgtt ttggaatgca ccgatgcctc
      841 agccagacca tgcccggcga gcctgtcaaa cggcgttgga gatgatcacc acactgcgtg
      901 agttacaggc cgactggaaa cggcgaggca tgccgatgct cgaagtcgga attggcatca
      961 actcggggcc ggcggtggta ggtaacatag ggtctatcga acgtttacag tataccgcgc
     1021 ttggtgacac cacgaatgtg gcctctcggt tggaagcatt aagtgtggtg tataaagtgt
     1081 ctattgtgat tggcgaattc actcgtcagg aagcgggtcc agccttcgag taccgcgagc
     1141 tggacttagt agccttgaaa ggccgggatg aaccactcgt cgtgtatgaa gtggttggtc
     1201 ttgctggcca acttaaccat gaccaagctc aggtgttgga acgctatcgg tgcgggcttg
     1261 atctctaccg atctcgtcgc tggagcgagg ctcaagcagt atttcgagat atccaagtca
     1321 aggcaccgga tgatggcccc actgccctat accttcatcg aagcacaaaa ttttgtgcta
     1381 atccgcctcc agccgattgg aatggcgtgt atgtggcaaa gaccaaatag ctccctctgc
     1441 caactcggtc aactgcttgg attcgcgggg ggagaccagg atgatgcctg gtaaagattc
     1501 ctgcaaggac cgcagttctt ccgcttgacc attcaagact ttggcggtct tgggattgct
     1561 cactggagtg gactccaact cctgggtgat gcgcgtgcta aggcgctccc ctttggtaat
     1621 ccgcccggca aagttttgtc gatagacttc cgtccctttg aagaaaccct ccaccataac
     1681 cgtaaacgta gtctccgctt ttccctctac aatcagcgtg aacggcccag gaacaccagc
     1741 tggcacctcg acggtgcgtg tgccgtatcc ctcaatccct gtgtgggatc caaagacctg
     1801 gttgacttcc aggcctgggg ccacaaaacc ggagactcga acgttgtctg gcattaacac
     1861 cagcgggagc agattgggag aggccgtcac ctttagagta ctgtggtgaa ccgcaaaagc
     1921 gtgccgacca gaatcctggc catcctgaac tttggtcttc tctccagact tcaaggtcac
     1981 taacgactgt cccattctgt cttccaccag gagatcacct gccaacgtcc aacagatgaa
     2041 actcgtgtcg gggttttgct gtccaccaat aagcccatca tgaaccgttg ccgttgcggt
     2101 attggattcc aaggagaagc tggaatcggg atcgacaaag ctgaccactc gggcccaaac
     2161 cgtcccaaga tggatccaaa tgctaatgcg tgagccttca tcacttacct cggccttttt
     2221 cacttccaca tcggaatccg gttgcacggt cagggtgctc ccatctaaaa acgttaccaa
     2281 ggccatacct tgagttccgg tcacaatccg gtcactgaca ttcaaactca tgccatcctt
     2341 cgcagcattt gattcagtgt tcccggcggg aataaggctc acagggccga caaggacact
     2401 cagcgtggcg gtagctgtca ccatggtggg ctcggtggct tgaaccgatc caggtccgta
     2461 caggaccaaa aacccaacga agcccctaaa gagggtcttg accaagctaa accgtcgaaa
     2521 aatgagccat ccctccaaat ggaaatttcg aaaattcatt gcaggattgg attgatctaa
     2581 tggaatcata ctatcttcct tcgctaatcg agagactgag tggtatgccc atgtaggctt
     2641 ccctcggttg tttggacaat ctttaaccct ttaggtttta acttcacttg caacgcttca
     2701 agccccgatg ggtttcggtc taaggccacg gtaacaccaa aacgctggaa cgcttaagag
     2761 atctcctgtg ccgggatgag atggagcagg ctgcggacat cagcagcttc ccggattcga
     2821 tgaaccatat tcctaacatc ctattcctcc ttcggtggaa tgtgtctcgc atcaattggt
     2881 gattttttca aaccatacgc ttactgctta ataatgtata gcttggcgac ttttctgtta
     2941 aatcccttgc gggtatattc cccgctgctt gcggcgtaaa ctactcgcgt gagcgagtac
     3001 attcataaga atcataatgg tacggtctta ctgtatcact tggtttttcc tgccaagtat
     3061 cgtcgggcga tctttgatgc gaccgtcgat aaagtattga aagatgtatg tctcgagatc
     3121 gagcaacgct atcaggtgaa atttctggag attggcacgg ataagcatca tgtccatttt
     3181 ctggtccaag cggtgccggg gtacagtgtg acgaaggtgg ggactctcat caagagtctg
     3241 tctgccaaag agattttccg tcgtgtccct caggtgaagg agcaattatg gggtggagaa
     3301 ttgtggactg acgggtatga cgcaagcacc gtggggaagc atggaaatga aacaatgata
     3361 ggaaagtatg tcaagcaaca aggggaggca taccagaagt tatacacaaa tcatcaatta
     3421 gtactcttct aataccccgt tgcttgcggc ggggttcttt atttccagtt ccgaggaaga
     3481 gaatccgtgc attgaaactt tgcaatgatg gaattgccta ttgttttagc agattggtga
     3541 taccaaacta aaaaatgcca gactattttc tacccgaata cagaaaacgg acgtgtgcaa
     3601 gagaatgcag gtgatttact gaaatgggga tggggctaaa aaattgtaac ctccggcatt
     3661 ctgctcccaa aggggagtat attttgcaac gctagtcagc atgatgcagc acagcaccac
     3721 agaatcatta atagcaacaa ggtctttcgt tgtttggtgt tcgttgccat agtaccttgt
     3781 ggcatggtct aaccgtgaca aaattcgtga attccaggtt ccaaccgtga caaaaaccgg
     3841 aaggaatttt ttttggctgg attttaaaag tcctcaccgt agcggacgag atgcgcaact
     3901 gctgtttgac ctctcaacta gaagactcca tgcctatcga agagatctta gctctcgtca
     3961 gttaaagaca aaaggcttga ggagaccagg aatcgaacct gggttctaag atccaaaatc
     4021 ccctatcaaa gaataatgat tcagaatgat tgagtaggat taagtgggag aaaacctatg
     4081 acaataaaga gattcatgcc tttaaagtac gattgctgga gattaaggta gactgaaaat
     4141 tgggacaata accgggacca acttttcaac aaattttcta tcaaaattct actgtttgca
     4201 tccccactca attcgcagaa ccccatcccc tgtggatagc ccattgaggg gtttcacgcc
     4261 ttcactccct ttttttatcc tgtacagaaa acagtcttta aaagagtggt tctcgaacga
     4321 tgctcataga ttttaacacc aagaaaattt gaggtgaggg cagtattttg atgagacacc
     4381 atttttctga gtagtttcaa aacaaaagat aattcctcga taagtgaagc tctattttta
     4441 tggaatccgg taattctacg aaaagtgggc ttacctgagt tttacggaca tataccaaat
     4501 gaaccacaat ataccctcca catctcacct ggcctcacga tggtcatatt gcagaagtcc
     4561 accgaatctt tcgggagagc gaatggggct ggttccccag ccagccctcc ttcctcgcac
     4621 gcggtagaaa tcattcattg aactttcctg tgggggcgct tccctagtgt aaacaggctg
     4681 ggcagacaag caggaaagga ttgctaaggc tttgaggtac atcctggatc gtgaccaaaa
     4741 ccgtgaccga accgtgacaa acttttccat caatactctg ccaaagcctt ccacccccaa
     4801 tggctttact cgggggtacg ggttggcaat ttaggggtcc cgcgttctca caacagcccc
     4861 caaaaggccc tggtgatgag agggctaagc acaaacttat caggggcaac ccattatcct
     4921 ttgttcatcc ccatcactaa aaacaaaaag cccgaccccg tgtgtaggca agtccgtgaa
     4981 ttttccaatt gaaataaaac ccatggggct ccaggacgat tttagaattt ttcgtatgcc
     5041 aaggccaggg ccagaattct ttgaattctt agctgcttag tacaaaattt tatcaaattc
     5101 caggataccc taatcgggta ttgccatgta tggacgctcc tgttgtcgcg cgatggcggt
     5161 atagtcgccg gaaccgcact ttacaaacag gagggactca tgccacacat caccacgatt
     5221 ggggttgatt gagcaaagcg tgtgtttctg gtgcacggga tcgatcatga aggacaagcg
     5281 gtgctacgca agaaattacg ccgagggcac gtgttgaaat tctttggaac gttaccgccc
     5341 tgcctggttg ggatggaagg ctgtgggagc gcccactact gggcccgagc gctcagcgcc
     5401 caggggcacc aggtgcgatt gatcccccca caatctgtcc gccccttttt gaaaaccaac
     5461 aaacatgatg cggcagatgc cgaagccctt gccgaggcct tggtgcggcc caccatgcgg
     5521 tttgctcccc ccaagagcgc ggaccaacaa gcggtgctga tgctccatcg gtcacgagag
     5581 ctcctggtca aaca
//
LOCUS       DATIAL010000486         6923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_410863, whole genome shotgun sequence.
ACCESSION   DATIAL010000486 DATIAL010000000
VERSION     DATIAL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410863"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..498
                     /locus_tag="VLA60_14915"
     CDS             106..498
                     /locus_tag="VLA60_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10699.1"
                     /translation="MFGPITLPFGAKMLFNTVKLKPGVSFDQVELAVGEMCMVVKETY
                     GGDKGGFIAGQVFKFSGFVSDKGSLSESKTTDDHYAIVTYWSSFEDHEKSHADELFNK
                     TFAALATMCSETKELGYDMLWQGAAKTA"
     gene            complement(632..1120)
                     /locus_tag="VLA60_14920"
     CDS             complement(632..1120)
                     /locus_tag="VLA60_14920"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HSF10700.1"
                     /translation="MQCPCQSGKTFKQCCEPIITGVKQAETAEQLMRARYSAYTQVQM
                     DFIEKTHDPKTIHDIDLEANRKWAETTKWTGLEILATKQGGRNDEAGLVEFKATFETE
                     EGPQIHHELSEFRKHKGTWYFTDGKSPGVQSVVRSEAKIGRNDPCPCGSGKKYKKCCG
                     NA"
     gene            1536..2009
                     /locus_tag="VLA60_14925"
     CDS             1536..2009
                     /locus_tag="VLA60_14925"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HSF10701.1"
                     /translation="MRYSQKFEIARFLAVLLDRRYTIPGTSIRIGLDPIIGLIPYVGD
                     TIVSVAGAFILIVAAQSQLPKIVLVRMSLNIAINGMVGAIPVLGDLFSVWFKSNVRNI
                     ELLERYAVGDRRTSTAGDWVFVVGLLLGIAFVVVGTVVGIIWLLTRVWEILQGMP"
     gene            2439..4622
                     /locus_tag="VLA60_14930"
     CDS             2439..4622
                     /locus_tag="VLA60_14930"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSF10702.1"
                     /translation="MKTQIQMVTAVMFIIVLVLFAPRIYALETARISVSTASVQSNNN
                     SFQPALNENGQLVVFHSFGSNLVAGDTNGDLDVFIRNRQTGETSRVSVATGGAQGNSL
                     SRAPDLSDDGNLVSFESDATNMVAGDTNAKRDIFVRDRQAATTTRVSVATGGGQVNGN
                     SFNSYLSGDGRFVAFDSVATNLVAGDTNAVSDVFVHDRQTGTTTRISVATGGVQANGG
                     SFLPALSMDGRFVAFHSTATNLVAGDTNGGTDVFVHDRQTGTTTRVSVATGGGQSTGS
                     GGSSPAFAPDISADGRFVVFESISNNLVAGDTNAVSDVFVHDRQTGTTTRVSVPTGGS
                     PQTNEESVEPAISGDGRFVAFLSYATNLVPGGTSGNGDVFVHDRQTNTTTQMSVSTGG
                     VKGNDFSCNPAISLDGHIVGMFSDSTNLVAGDTNGARDIFIRDRSVTFDLDGDGKADI
                     VWRNTSSGSVEAWLMNGTALANAGFPGDLPLAWEIAKVGDVNGNGKADIVWRNTSNGA
                     VAVWLMDGLTITSVGFPGSSPTNWEIGQIGDLDRNGTADFVWRNTINGATALWLMNGA
                     AIKSTAFPGGVPLAWQIVGIADVNGDGRADVIWRKNTGVVALWLMNGSTITSVGFPAT
                     VPSGWNYEGSGDVDGNGTRDLFWRNASSGTVAVWLMNGATIASSANVANLASDREIEQ
                     VADTNGDGKPDLIIRNTATGAVSVLVQGGGSGFPGTKSLDWEIQN"
     gene            complement(4903..6201)
                     /locus_tag="VLA60_14935"
     CDS             complement(4903..6201)
                     /locus_tag="VLA60_14935"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HSF10703.1"
                     /translation="MPAYSEESQAVEQLEKMIEKPKAARKSLKDEGILLEATNTMDIF
                     SNISGGIHRKTTVTGDLDLLLTLDIKKLIPSWRGTVFVYGLGLYGDDPSKNVGDIQGV
                     SNIAAPNTWKLFEAWYQHNLFKRFSLLAGLYDITSEFDVIVSASTLFLNSSFGTGAEL
                     GTGGRNNLSTFPATSLGIRTQAILTDSLMVRAVVADGIPGNPDDAHGTHIHLRKDDGL
                     FGSMELAYYQYSKEDLTEPKKEMVKESPRRLTFRRIGRSAPIEYEAKVAYGFWGYTTS
                     LDQLNKVNSSGKPVKQHGTYGMYGLAEYDVYHEKEDKDQGLTLFGRAGMADPNVNRIS
                     QYYGGGFVYKGLFPGRKIDGTGMGVAVAVNSHDYKQSQRRAGQRVESSEITLELTHAI
                     FVNQNLIIQPDFQYVINPGTVPGRNNAVVVGVRLEVNLNN"
     gene            complement(6440..6907)
                     /locus_tag="VLA60_14940"
     CDS             complement(6440..6907)
                     /locus_tag="VLA60_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HSF10704.1"
                     /translation="MPIWVDADACPRVIKEILFRAADRTAVQVTLVANRLLYTPPSPY
                     ISAIQVADGFDVADSEIVKRLEAGDLVITADIPLAAEVIAKGGHALDPRGEMHSSDTI
                     QARLMMRDFMETLRASGVETGGPSALSQRDRQLFAKHLDYYFTILVKQKNENP"
BASE COUNT         1577 a         1629 c         1944 g         1773 t
ORIGIN      
        1 tgcggtaagt gctaaaatga tgatgagttc tgcggatagg gttagtctga ttattccctt
       61 tacaggcatc cgtttcgaga cgggtgctct caccaggagg gtgccatgtt tggtcctatt
      121 actcttccat ttggggcgaa aatgttgttt aacaccgtca aattgaagcc cggcgtttcc
      181 ttcgatcagg tggagctggc cgttggagag atgtgcatgg tcgtcaagga aacctatggc
      241 ggagacaaag ggggcttcat cgccgggcag gtgttcaaat tttccggttt cgtatctgac
      301 aaagggtccc taagtgaatc aaaaaccaca gatgaccatt acgcgattgt gacttattgg
      361 agttcatttg aggatcacga aaaatctcac gccgatgaac tgttcaataa gacattcgcc
      421 gctttggcta cgatgtgttc tgaaacgaaa gaactcggct acgatatgct atggcagggg
      481 gcggcgaaga ctgcataagc ttttcgacac agaaagatct ccttcaccca tctggccagg
      541 cgtcggtcag tttggcgcgt gtttacagag gataatgtca tgccgggaca ggagtgcgtg
      601 aaacggacgc gatatggaat cccatcctga ttcaggcgtt tccacagcat tttttatact
      661 ttttcccgct gccgcagggg cacggatcgt tccgtccaat cttggcctcg ctacgcacga
      721 cggactgaac tccaggactt ttgccgtcgg tgaaatacca ggtcccctta tgtttccgga
      781 actcgcttag ctcatgatgg atttgagggc cttcttcggt ctcaaacgtg gctttgaatt
      841 cgacgagtcc cgcttcatcg ttcctaccgc cctgttttgt cgctagaatt tctaaccccg
      901 tccacttggt ggtttctgcc cacttgcgat tggcctcaag gtcaatgtcg tgtatggttt
      961 tggggtcatg ggtcttttcg atgaaatcca tctggacttg cgtataggcc gaatatctgg
     1021 cccgcatgag ttgttcggca gtctctgcct gtttcactcc ggtgataatg ggctcacagc
     1081 attgcttgaa ggtctttccg ctctgacacg gacactgcat atgcgactcc ttttctaagt
     1141 gaaggcaaat gatgatggat tcttgtacgt cgccagtgac agaaaagtca aaaggcccgg
     1201 catctcattt ttctcgaaaa atattggtga gtcccggcag cagacttccc gtcatttgtc
     1261 tctgctcgtc cctctcttat ataactggtc tctctctgtc gctaaagcca tgcccggtgg
     1321 acattgttga atgcggtgta cctcacgtgg cccgcaattc aggatttagc ggaaaaatgc
     1381 ttcaatgatc cttggctgca gaggacgtac atttttcttc tcctgtgtct gttgttagtg
     1441 aagcagatct ggttatattg ttcattactc cgaattatgc tgattaaggt gccaatttat
     1501 acattggcag aatgaaaagg gcgggaactc ctatcgtgcg atattcccaa aaatttgaaa
     1561 ttgccaggtt tctagcggtc ttgctggatc ggcgctatac cattccggga acatcaattc
     1621 ggattggttt ggatccgatc attggtctga tcccatatgt gggcgatacc atcgtgagtg
     1681 tcgccggggc cttcatcctt atcgtggcgg ctcaatccca acttcccaaa attgtcttag
     1741 tacgaatgag tctgaacatt gcgattaacg gcatggtagg tgccattccc gttctcgggg
     1801 atcttttttc ggtctggttc aaaagtaatg tgagaaatat cgaattgttg gagcgatatg
     1861 cggttggaga tcgccgtacc tcaaccgcag gggattgggt tttcgtggtg ggactattac
     1921 taggaatcgc tttcgtcgtg gtgggaacgg tcgtggggat tatctggctt ctcacgcgtg
     1981 tctgggaaat cctacaggga atgccatgaa tgttcggctt attacaaggc aggaagaatg
     2041 taatcccgtg tgtcgcctgt cgatgatcga tctgcttgaa gcctgatccc aaatcttgaa
     2101 cggtcgtcca cagataaggg cctaaccttt atcattttca gtttacatgg cttttcattt
     2161 catccggctt tcatgcccat tcctgtgtgc ccattgggtt tgaacgcctt tcatttcaga
     2221 acgtgtggat attattggcc aattgagtta gggttttttc gggtggatcg ccagaagaga
     2281 ttgttgggat gtggtcaaca aagagaatga aaccgatctt ggaaatttca tgtgggatgc
     2341 tcatcataat ttgggaaata cctcattata aatgcgggtg atgggtccat gtggtggttt
     2401 tattggcttt atattcggca gactcgggag ggaaaaccat gaagactcaa atccaaatgg
     2461 tcaccgcggt catgttcatc atcgtgctcg ttctcttcgc tccacgaatt tatgcactgg
     2521 agacggccag gataagtgtc agcacggcca gcgtgcagtc caataacaat agttttcagc
     2581 cggccttgaa tgaaaacggc caattggtgg tgttccattc ttttgggtcc aacctggtag
     2641 cgggtgatac caacggtgat ctagatgtgt tcattcgtaa ccggcagacc ggggagacct
     2701 cacgggtgag cgtggcgaca ggaggagccc aggggaattc cctcagtcga gccccggatt
     2761 tgagtgatga cggaaatttg gtctccttcg agtccgatgc aaccaacatg gttgccgggg
     2821 ataccaatgc aaaacgagac atattcgtcc gcgaccgcca ggcggccacg accaccaggg
     2881 tgagcgtggc cacgggtggg ggacaggtga atggcaacag ctttaattcc tatttaagcg
     2941 gggatggtcg atttgtggca tttgattcgg tagccacgaa tcttgtggcg ggggatacga
     3001 atgccgtcag cgatgtgttt gtgcatgacc ggcagacggg cacgaccacg cggatcagtg
     3061 tggcaaccgg gggcgttcag gccaatggtg gaagtttttt acccgcgctc agcatggatg
     3121 gtcgatttgt ggcgtttcat tctacggcca ccaacctcgt ggcaggagat accaatggag
     3181 gcaccgatgt gttcgtgcat gaccggcaaa cgggcacgac tacgcgggtg agtgtggcca
     3241 cgggtggggg gcagagcacg ggatcaggag ggtcatcgcc tgcctttgct cctgatatca
     3301 gtgcggacgg gcgattcgtc gttttcgagt cgatatcgaa taacctggtg gcgggagata
     3361 cgaatgccgt cagcgatgtg tttgtgcatg accggcagac gggcaccacc acgcgggtca
     3421 gtgtgccgac aggcgggagt cctcagacca acgaggaaag tgtcgagcca gccatcagcg
     3481 gggacggacg gtttgtggcg ttcctgtcct acgccacgaa tcttgtgccg ggcgggacga
     3541 gtggaaacgg agacgtgttc gtccacgatc gccagacaaa cacaacaacc cagatgagtg
     3601 tgtcgaccgg aggggttaag ggcaatgatt ttagttgtaa tcctgccatc agcctggatg
     3661 ggcatattgt cggaatgttc tccgattcga ctaatctcgt tgcaggcgat actaatgggg
     3721 cacgggatat ttttatccgt gatcgttctg tgaccttcga tctggatggg gacggtaaag
     3781 cagatattgt ttggcgcaac acgagtagcg ggagcgtaga ggcatggctg atgaatggga
     3841 cggccttggc caacgccggt ttcccggggg acctgccgtt ggcatgggag atcgcaaaag
     3901 tgggtgatgt gaatgggaat ggcaaagcgg atatcgtgtg gcgcaacacg agtaacgggg
     3961 ccgtggccgt atggttgatg gatggactga cgatcacgtc ggtgggattc ccgggaagta
     4021 gtcccacgaa ttgggaaatt gggcagatcg gggatctgga caggaatggc acggcggact
     4081 tcgtctggcg caacaccatc aatggcgcca cagcgctctg gttgatgaat ggcgcagcga
     4141 ttaagtccac cgctttcccc ggcggtgtgc cgttggcgtg gcagatcgtg gggattgctg
     4201 atgtgaatgg agatggcagg gctgatgtca tttggcggaa aaacaccggc gtcgtggcgt
     4261 tatggttgat gaacgggtcc acaatcacgt cggtgggctt tccggccact gtcccctcgg
     4321 ggtggaacta tgaggggtcc ggggatgtcg atggcaatgg gacaagagat ctgttctgga
     4381 gaaatgccag tagtggaacg gttgcggtgt ggttgatgaa tggagcgacc attgcctcat
     4441 ccgctaacgt agctaacctg gcctctgacc gggaaataga gcaagtggca gataccaatg
     4501 gggatgggaa accagacctc atcataagga ataccgccac cggggcagtg tcagtgttag
     4561 tgcagggggg agggagtgga ttccccggta cgaaatcgct tgactgggaa atacaaaatt
     4621 aggtcgatca tttcgtggag gatgactaat caggcatctg actccgaagg gttcggactg
     4681 aggaaagcca aaagggtctg accttcctgg aaggtcagac ccctactctc aggggcagac
     4741 cgttcaggtg gtggcagaga tggtgaatga aaatttcgga aaaaccaaaa aactatagca
     4801 tcgcgtgtct acttgatcga agccttctta tatgggcgac gtctttgatc ctgtttaata
     4861 tcccctattt ccccaatcac gagatcctga gaagggggtg aatcaattat tgagattgac
     4921 ctcaagccgc acgcccacca caacagcgtt attcctgccg ggaaccgtgc cgggattaat
     4981 gacatattgg aaatccggtt gaatgatgag gttttgattc acaaagatgg catgagtcaa
     5041 ttccagcgtg atctccgaac tctcaacccg ttgtcctgcc cgccgttgag actgtttata
     5101 atcatggcta ttcaccgcga cggccactcc catcccggtt ccgtcaattt ttcgccccgg
     5161 aaataatcct ttgtagacga acccgccgcc ataatactgg gaaatgcggt tcacattggg
     5221 gtcagccatt ccggctcgac caaagagagt cagcccttga tctttgtcct ccttttcatg
     5281 atacacatca tattccgcta atccatacat gccgtaagtg ccatgttgtt tgacaggctt
     5341 tccggaacta ttgaccttgt tgagttgatc cagagaagtg gtgtaccccc agaatccgta
     5401 ggcaactttg gcttcatatt caatgggggc ggatcttcca atccgacgaa aggtcaggcg
     5461 gcgagggctc tctttaacca tttccttctt gggttctgtt aaatcctctt tgctatattg
     5521 gtaatacgcc agttccatgg aaccgaacaa gccatcgtcc tttcgcaaat ggatgtgggt
     5581 accgtgagca tcatcgggat tacccggaat gccatcggca accacggcgc gaaccatgag
     5641 agaatcggtg aggatggctt gcgtcctgat tcccagagag gtcgcaggaa aggtggacag
     5701 gttgtttctg ccacccgttc caagttccgc tccggtcccg aatgagctat tcagaaaaag
     5761 cgtcgacgcg gatacgatga catcaaactc ggaggtgata tcatacaggc cggcaagcag
     5821 agaaaagcgt ttaaaaagat tgtgctggta ccaggcttcg aacagtttcc atgtatttgg
     5881 tgccgcaatg ttgctcacgc cttggatgtc accgacattt ttgcttggat cgtccccata
     5941 caagcccaag ccatacacaa agactgttcc tctccaacta ggaattaatt tttttatatc
     6001 cagtgtgagc agaagatcca gatccccggt gacagtggtt ttccgatgaa tgccgccgga
     6061 aatattggag aagatatcca tcgtattggt ggcttccaga aggattccct catccttcaa
     6121 actttttcgg gcagcctttg gtttttcgat catcttttcc aattgctcaa ctgcctgact
     6181 ttcttcagaa taggctggca cgggaatcaa caataccaaa aggaataacc cgcagagggg
     6241 gtgaaggatt attcttgttc gacagaattt catgctgggg tatcaggctt ctaccggttt
     6301 ggaccttcag tttttgtgga taaggctatt gtctgcgaaa tcgagttttt attgatgccg
     6361 taaatcaaac ccgtgagggc aggcagggtc agcaatggtt tatggcctgt caccataccc
     6421 catgggtggt ttccgattcc tagggattct cattcttctg tttgaccaga atcgtgaaat
     6481 agtaatccag gtgtttggcg aataattgcc ggtcccgttg gctgagggcc gacggaccac
     6541 cggtctcgac gccgctggct cgaagcgtct ccatgaaatc ccgcatcatg aggcgggctt
     6601 gaatcgtatc ggaagaatgc atctccccgc gcggatcaag cgcatgcccg cccttcgcaa
     6661 tcacctcagc ggccagggga atgtcggcgg tgatcaccag gtcgccggcc tcgagccttt
     6721 tgacgatttc tgaatccgcg acatcaaatc catcggcgac ttggatggct gaaatatagg
     6781 gcgagggagg ggtatacaaa aggcggttgg ccacaagcgt cacctgcacc gctgtccggt
     6841 ccgcggcgcg aaataatatt tctttgatca ccctggggca ggcatcggca tcaacccaaa
     6901 ttggcatggt cgaccttcat ggt
//
LOCUS       DATIAL010000487         6606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1486256, whole genome shotgun sequence.
ACCESSION   DATIAL010000487 DATIAL010000000
VERSION     DATIAL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486256"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VLA60_14945"
     CDS             complement(<1..873)
                     /locus_tag="VLA60_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HSF10705.1"
                     /translation="MKVSALAGKPTQPSMLANIPRLVTAYYTGRPDPAVPEQRVAFGT
                     SGHRGSSLKNSFNEAHILATTQAICLYRQQQHIDGPLFLGMDTHALSEPAMASTLEVL
                     AANGVIVMVDRDNGYTPTPVISHAILTYNRGRKTGLADGIVITPSHNPPEDGGFKYNP
                     PHGGPADADVTTWIEKQANALLADDLRGVQRVPYEKAQRASTTRKHDYVGSYVGDLGA
                     VIDMEAIRDAKLSIGVDPLGGAGVDYWGPITERYGFPVTVVHEGVDPTFRFMNLDWDG
                     KIRMDCSSPYAMAGL"
     gene            2042..3412
                     /locus_tag="VLA60_14950"
     CDS             2042..3412
                     /locus_tag="VLA60_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HSF10706.1"
                     /translation="MDSFESFVATVAGWVWGPPMLLLLVGTGLYLTILLEGLQFRVLP
                     HALKLIFHKEPSSNGDISHFAALMTALSATVGIGNIVGVAAAITLGGPGAVFWMWMTG
                     LVGMATKYGEAVLAVKYREQGKYGMRGGPMYYLANGARLPWLGWLFALFTACAAFGIG
                     NMTQANAVAGILESTFQVQPWITGVVLMGMTGLVILGGITSIGRFTSVLVPFMIVGYV
                     TSALVVLALHVTEIPQALASIVFHAWNPIAAGGGFAGATMAAAMRYGLARGVFSNESG
                     LGSSPIAAAAARTDDPVRQALVSMTQTFIDTLVVCSMTALVILTATPWTQGIDAAQLT
                     SASFGETLGHTGELIVTLSITLFAYSTLIGWNYYGEKAIEYLMGPGAIVYYRVIFVAV
                     VLVGATSRLELVWNVSDVMNGLMAIPNLIGLLLLAKIIKEETTRYFSTQKLGAGSSRS
                     STPPPV"
     gene            complement(3568..3858)
                     /locus_tag="VLA60_14955"
     CDS             complement(3568..3858)
                     /locus_tag="VLA60_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10707.1"
                     /translation="MGDPFTINTLFVYTVRMALPSDLSFSDSLKSARRQAFYEHHKRI
                     ALAMILIVFLLPLIGVFMSGLSGAISGVVISVLSFYLAPYALLKLGQWFVHS"
     gene            4142..4660
                     /locus_tag="VLA60_14960"
     CDS             4142..4660
                     /locus_tag="VLA60_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF10708.1"
                     /translation="MATKTLKAQAAQVTDMLRKDHKKVKGLFGKFEKTNNAQEKQEIV
                     DTILTELEIHAELEEKLIYPAIRPKMNDEDLMDEAIEEHHVVHGVIGELKKMKPAAER
                     YDAKVTVLGELCKHHIKEEEEEMLPKAEKRDIDWDRLYEQVMKRKDQLMAKAGLSSNN
                     GSPTNSKARKKK"
     gene            4737..5057
                     /locus_tag="VLA60_14965"
     CDS             4737..5057
                     /locus_tag="VLA60_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HSF10709.1"
                     /translation="MPDENTLPLGMLVLLLLVSVAIAQGVREEALMVGDRVLLPCSSR
                     PNCVSTVDAGEIHAIAPYRNHTSLNEIKDVLKQVFSEYPRMEVIKDEEDYLRYESAIF
                     SWVL"
     gene            complement(5240..>6606)
                     /locus_tag="VLA60_14970"
     CDS             complement(5240..>6606)
                     /locus_tag="VLA60_14970"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HSF10710.1"
                     /translation="PFIQEYTEGFDNFATYTRNMSWEKIEEGSGIAREDIQKAADIAE
                     QADSIICTWAMGMTQHKNAVANMQEIINFLLLRGNIGKPGAGPCPVRGHSNVQGDRTM
                     GITENPTPEFLDRLEKVCHFQPPTKRGHDAVRAIKAMHAGKAKVFIALGGNFLSATPD
                     TAYTAKALSRCRLTVHISTKLNRAHLVTGTEALILPALGRTDKDLQDDIPQYVTTENT
                     MGVVQTSQGRLEPVSESLKSEVDIIASLAKATFNNKEGPGNHINWDELIHDYDEIRHL
                     ISQVVPGHDHYTERVNKPGGFYLANPIRDARQFPTPSGKARFTVHPMPDIHLGPNQLL
                     MMTIRSHDQYNTTIYGLDDRYRGIKAGRRVVFMNERDMEERHLAKGDLVDIVSHHEGQ
                     TRTAPQFVVVPYPIPRTCTATYFPEANVLIPIDSVADKSNTPTSKSIVVTIHPTTLTH
                     PSHT"
BASE COUNT         1526 a         1647 c         1825 g         1608 t
ORIGIN      
        1 cagtccggcc atggcgtagg gtgaggaaca atccatgcga atctttccat cccagtccag
       61 attcatgaag cgaaaggttg gatccacgcc ttcatggacg accgtgaccg ggaatccgta
      121 ccgctcggtg atgggtcccc aatagtccac gcctgcgccg ccgagcggat ccaccccgat
      181 ggacagtttc gcgtcgcgga tggcctccat atcgatcaca gcgccaagat cgccaacata
      241 tgagccgaca taatcatgct tgcgtgtggt cgaggcccgc tgcgctttct catacgggac
      301 gcgttggact ccgcgcaggt catccgccaa cagtgcattc gcctgtttct cgatccaggt
      361 ggtcacgtcg gcatcagccg gcccgccatg gggcgggttg tatttgaagc ccccgtcttc
      421 cggcggattg tgcgagggcg tgatcacaat gccatccgcc aatcccgttt ttcgacctcg
      481 attgtaggtc aagatggcat gggaaataac cggggtcggg gtgtatccgt tgtcgcggtc
      541 caccatcaca atcacgccat tggcggccag tacttcgagc gtgcttgcca tggcaggttc
      601 cgaaagcgcg tgggtatcca tgcctaaaaa gagagggccg tcaatatgct gttgttgacg
      661 atagaggcag atggcctggg tggtggccag gatatgtgct tcattaaagg aattctttaa
      721 cgaggagccg cggtgccccg atgtcccgaa ggcgacccgt tgctccggca cagccgggtc
      781 gggtcgaccg gtgtaatagg cggtaaccag ccgggggatg ttggcgagca tggacggctg
      841 ggtgggcttt cctgcaaggg cactgacctt catggtgatc gctccgcgtt caaaaatgga
      901 tgaatgtgct atctcctgct ttcagagaaa ggaggcatac cttaccgaga ttcatcagag
      961 gattcaaagc cttcattctt tctcctgccg acctggcggg gtcccggaaa tttcacgcag
     1021 acttcgactc cgttttggat ttgttctgta gggcatgaat ccgcaccaaa ggcatggaaa
     1081 tctcagacat catggccgtc ttatcaacat ctttaaggaa attctgtgga taagaaaatc
     1141 attgacagcg tattttttat gacggacatc aatgggcatc ggtatggaga cgtatgtcaa
     1201 gaaaaaaagt cctatttcta cctttactcc tacgccaaaa aacctacacc acttcctgtg
     1261 ggggcataaa aaaatcttta tacaatgcct aaaacagtgg caatctgctc atttcctttt
     1321 tattccttag aaaatcacgc gatgcagcac agtattcacc aggttatgca caaaatttgg
     1381 ggatgaattt tttttgccat ggtctgcatt ttgccgaccc caaatatcaa agctttttat
     1441 gtgatggctg ccattcgcga ggagccaaag ggatgatggt tcaaatcatc tcaaaactat
     1501 tcccatcctc gttcgtctcc aacttttgcg ccaaccctta acgaattcaa tagaagataa
     1561 ttgttgatgt gccccagttt tgatcatagc taacaccccg atatcccatg agcggtgtta
     1621 tcctgagtgg aacgaaggat ttcgctagcc gtgtcatctt taattttggc acagatgctt
     1681 cgccatgggc tcagcatgac aagttggcga gggccttgcc tggtgcggat gttcttcaaa
     1741 tgtgcgaatg tcccattgaa ggttttgccc tgcaaggggc acgcctcatt tttgtgaaga
     1801 gccatgccac aacgattcac accatggctg tttacacaat gcacctcatt gatagaataa
     1861 gccgtgcctc gcaattattc ctccgcccct ttcttgcttg ttccacgcac ggcgtttcgc
     1921 cgtttatttc ctgcaaacca ttccgccgcc tatgtcgtgc cagaggtggg cgtgccattg
     1981 ttgatgagaa tgccttcgag cccttatctc agacctttcc gctattggag aatcaggcct
     2041 catggactcc tttgaatcct tcgtagcgac cgtagccgga tgggtgtggg gtccgcccat
     2101 gttacttctg ttggtcggca ccgggttgta tctgaccatt ctgctggagg gcttgcagtt
     2161 tcgtgtcctg ccgcatgctc tgaaattaat tttccacaaa gagccgagta gcaatggaga
     2221 catcagccat ttcgccgcgc tcatgacggc cttatcagcc acggtgggga tcgggaatat
     2281 tgtcggcgtt gccgccgcga tcacgctcgg cggacccgga gccgtctttt ggatgtggat
     2341 gaccggcctg gtgggcatgg ccaccaaata tggcgaagcc gtgctggcgg tgaaataccg
     2401 tgagcagggg aaatatggga tgcgtggcgg gcccatgtat tatctagcca acggtgcccg
     2461 gctaccctgg ttaggctggc tctttgcctt gttcacagcc tgtgcggcgt ttggcatcgg
     2521 caacatgacg caggctaatg ccgtggccgg cattcttgag tcgacctttc aggtccaacc
     2581 ctggatcacc ggcgtggtgt tgatgggaat gaccgggctg gtgatattgg gcggcatcac
     2641 atccattggc cggtttacct cggtgttggt gccattcatg atcgtggggt atgtcacgtc
     2701 agccctggtc gtcctggccc tgcatgtgac ggagatccca caagccttgg ccagtatcgt
     2761 ttttcacgca tggaacccga ttgctgcagg aggcgggttt gccggtgcca cgatggccgc
     2821 cgccatgcgc tacgggttag cgagaggcgt gttttcgaat gaatcgggtt tgggctcctc
     2881 gccgattgcc gctgccgctg cacggacaga cgatccggtc cggcaggccc tggtcagtat
     2941 gactcagacc ttcatcgata ccctggttgt ctgcagtatg acggccttgg tcatcctgac
     3001 cgcgactcca tggacacagg gtatcgatgc ggctcaactc accagtgcca gtttcgggga
     3061 aaccctgggc cacacgggag aactcattgt caccctctcc attaccctgt ttgcctattc
     3121 gaccttgatc ggctggaatt attatgggga gaaagccatt gagtatttga tggggccggg
     3181 cgccatcgtg tattaccgcg tgatctttgt tgcggtggtc ctggtcggtg ccacgtccag
     3241 attggaattg gtttggaatg tgtccgatgt catgaacggc ctgatggcca ttccgaatct
     3301 catcggattg ctgttattgg caaagattat taaagaggaa acgacgcgct acttcagcac
     3361 gcaaaagcta ggcgcaggtt cctcgcggtc ttctactccc cctcctgtgt aaaagtgggg
     3421 aaggggaaac tgcaaaccct cattaaacaa ccagcagatc aaccatccgg gtgcagatca
     3481 ttcgttgcgt ccaacccggc cgaatttttc tgtacggttg aggggtggca ctcctattca
     3541 ccacatggtg taaagttgga ccgggcatca ggagtggaca aaccattgcc ccagcttcag
     3601 taacgcgtaa ggtgccagat aaaaagacaa cacggaaatc acgacccctg aaatggcccc
     3661 ggacaatccg ctcatgaaca ccccgatcaa gggaagtaaa aacacgataa gaatcatggc
     3721 gagagcaata cgtttatggt gttcgtagaa agcctgtcgc cgggcactct tcagagaatc
     3781 ggaaaaggat aaatcggaag gaagcgccat ccgcacagta tagacaaaca gtgtgttgat
     3841 tgtgaatgga tcccccaacc ttggctcccg ttccttacaa aggatgtttt attttgaaga
     3901 gaaatgaaag ggagggaggg gcgctattct tcccaacgcc ccacctcaca cagacgaaag
     3961 agatccacac ctccccaaag cctacgctcc cgggaaccat acatcctaaa gacctttgaa
     4021 taaaagttag gtaacctcac ccgttttata cattgggcgc atgacattct cctgatataa
     4081 atcaataagg aagagagcgc gagtgatgaa tgcaaaattt tactaaaagg agaagtcgtc
     4141 catggccaca aagactctga aagctcaggc agcacaagtc acagacatgc ttcgaaaaga
     4201 ccacaaaaaa gtcaaagggc tttttggaaa atttgaaaaa acaaataatg cccaagagaa
     4261 acaagagatc gtcgacacga tattgacgga gttggaaatc cacgcggagt tggaagagaa
     4321 actcatctat ccggccattc gcccgaaaat gaatgacgaa gatcttatgg atgaggccat
     4381 cgaagagcac catgtcgtgc atggtgttat cggagaactg aaaaaaatga agcccgccgc
     4441 cgaacgttat gatgccaaag taaccgtact cggtgaattg tgcaaacacc atattaaaga
     4501 agaagaggaa gaaatgctgc ccaaggcaga aaagcgggat attgactggg accggttgta
     4561 tgagcaggtc atgaaacgga aagatcaact tatggcgaag gcggggttgt cttccaacaa
     4621 cggctcaccc acaaattcga aggcgaggaa gaagaaatag gaaacgtcac acacgggtgc
     4681 gatcaaccgc atgtatcaaa agacattttt ataagtccct tgcgcgcagt ccaaccatgc
     4741 ccgacgagaa cacactcccg ctgggaatgc tggtcctatt actccttgtt tcggtagcca
     4801 tcgcccaggg ggtgagagag gaggcattga tggtagggga tagggttctc ctcccatgct
     4861 caagccgtcc taactgcgtc tccaccgtgg atgcggggga aatccatgcg atagcacctt
     4921 atcggaatca cacatctttg aatgaaatca aagatgtgct gaaacaggtc ttcagtgaat
     4981 accctcgcat ggaagtgatc aaggatgagg aggattacct tcggtacgag tcagcaattt
     5041 tttcttgggt tttatagacg aggtcgattt cgtgttcgat gaggccacca aaatcataca
     5101 cttccgttcg gccgcacgta agaggtctac tttcggctaa taaaaacctt tgcccaatgc
     5161 gaactgctta aggctgagcc ggatccttcg gaaacctcag gatgacaacc ttttgaaggg
     5221 cttctgtgta tttgaccgtt taagtgtggg aaggatgagt gagtgtggtg gggtggatgg
     5281 tgacgacgat ggatttggag gtcggcgtgt tgctcttgtc cgccacgctg tcgatgggga
     5341 taagcacgtt ggcttcgggg aaataggtcg ccgtgcaggt gcggggaatg gggtagggga
     5401 ccacaacaaa ttgtggtgct gttcgcgtct gtccttcgtg gtgactgacg atatccacca
     5461 ggtctccttt cgctaagtga cgctcttcca tatcacgctc attcatgaac accacccgtc
     5521 gtccggcttt gatgccccgg taccggtcat ccaatccata gatggtcgtg ttgtattgat
     5581 cgtgactgcg aatggtcatc atgagcaatt gattcggtcc taggtgtatg tccggcatcg
     5641 ggtggacggt gaaccgcgct ttaccagacg gagtgggaaa ttgccgggca tcgcggatcg
     5701 gattggcgag atagaaaccg ccgggtttgt tgacccgttc cgtgtagtga tcgtgtccgg
     5761 ggacgacctg gctgatgaga tgacgaatct catcgtaatc gtgaattagt tcgtcccagt
     5821 tgatgtggtt ccctggccct tctttgttgt tgaacgtagc cttagcgagg ctggcgataa
     5881 tatcgacttc acttttgagc gactcggata ccggctcgag tcgtccctgc gaggtctgga
     5941 cgacacccat ggtgttttcc gtggtgacat attgcgggat atcatcttga agatccttgt
     6001 cggtgcgtcc aagcgcgggg aggatcaagg cctcggttcc ggtgaccagg tgggcccgat
     6061 tgagtttggt ggaaatgtgg acggtgaggc ggcagcggga aagcgccttc gcggtatagg
     6121 ctgtatccgg cgtggccgaa agaaagttgc cgccgagggc gatgaagacc ttggcttttc
     6181 ctgcatgcat ggccttgatg gctcgcactg cgtcatgacc tcgtttggtg ggtggctgaa
     6241 agtgacagac cttttcgagg cgatccaaaa attcgggagt ggggttttcg gtgatgccca
     6301 tggtgcgatc gccctgcaca ttgctatgcc cacggacggg gcaggggcct gcgcctggtt
     6361 ttccgatgtt gccgcgcaac aggaggaagt tgatgatttc ctgcatgttg gctacagcgt
     6421 ttttatgttg ggtcataccc atggcccagg tgcagatgat actgtcggct tgttcagcaa
     6481 tgtccgccgc tttttgaatg tcttcgcgtg caatcccgct gccttcctca attttctccc
     6541 aagacatgtt acgtgtgtaa gttgcgaaat tgtcaaagcc ttcggtgtac tcctgtatga
     6601 acggca
//
LOCUS       DATIAL010000488         5577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_625422, whole genome shotgun sequence.
ACCESSION   DATIAL010000488 DATIAL010000000
VERSION     DATIAL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625422"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VLA60_14975"
     CDS             complement(<1..466)
                     /locus_tag="VLA60_14975"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HSF10711.1"
                     /translation="MPYQPIENYGLIGNMQTTALVGMNGSIDWFCYPYFDSPSVFCAI
                     LDDAKGGRFKIAPVPDGVKSKQYYWPDTNVLVTRFHTPAGVGRVIDFMPVGVRTSDCI
                     RDGLIRRVQAIRKSMTFRLECRPAFNYGRESHTLTITKDGTRFQSSQLTLRLG"
     gene            561..1493
                     /locus_tag="VLA60_14980"
     CDS             561..1493
                     /locus_tag="VLA60_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10712.1"
                     /translation="MSRTPRYHASVFMVGEILIQMVYHGIRLSTGAENTMGMTRSYPP
                     VVEIYGAEYGYERILKDDFFSVNVLYRNQEGERYVLKLSDFRFVLGWLFRPLTGWISR
                     REYRIYQMVSDLPGIPALGPQYGKRGYFHRFIEGHTLHEIERLIQEQFKVVVGDPAMA
                     TYATTLAPDFFDQLRGIVQSVHRRRIFYADLNKRGNIICSTEGKPYLIDFQICLPVPV
                     RQGWVGALTEKIFQRLIREDLYHLYKHKHTFQPQNLSEKELQLAQRSHLNTRYHRYLW
                     RPYIALKRLIYPHGSNETIWYKWKKEKDQSPRMS"
     gene            complement(1611..2132)
                     /locus_tag="VLA60_14985"
     CDS             complement(1611..2132)
                     /locus_tag="VLA60_14985"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HSF10713.1"
                     /translation="MREAKNRDIWIVILMGVSGSGKTTVGQLLARKLGWSFYEGDDFH
                     SPSNVSKMLGGVPLSDEDRLPWLLAIHQLVHDLISQSQRAVITCSALKQTYRKLITEG
                     HPQVALVYLRGNYALLHPRVQSRTDHFMKADLLASQFTALEEPHDVPFIDVSQAPEVI
                     VEQIKRMLHLQPA"
     gene            complement(2142..2681)
                     /locus_tag="VLA60_14990"
     CDS             complement(2142..2681)
                     /locus_tag="VLA60_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HSF10714.1"
                     /translation="MMDQPFLTDVQTLRKRAREHMEKGAVTPGYAADRDTVIKLLNEA
                     LATEIVCVLRYKRHYYMASGIHAQSVAAEFLQHANEEQGHADQLAERIVQLGGEPNLS
                     PEGMLMRSHSEYVEGTSLVDMIKEDLVAERIAIDSYREVINYLNTKDSTSRRMLEGIL
                     AVEEEHADDLVSLLEEMGS"
     gene            complement(3360..3914)
                     /locus_tag="VLA60_14995"
     CDS             complement(3360..3914)
                     /locus_tag="VLA60_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HSF10715.1"
                     /translation="MMDTLPKNHFFNPWHHATPGDKVPEFVNGIIEIPKGTRAKYELD
                     KESGLLRLDRVLYSSVYYPANYGFIPQTYCEDKDPLDILIISQIDVVPMCIVPAKVIG
                     VMRMLDNGEADDKIIAVAAGDPSVSHIKDISELPQHFISEMRHFFEEYKTLEHKAVVV
                     EEFLEKKVAQNILNQSLSMYKDLF"
     gene            complement(4598..5086)
                     /locus_tag="VLA60_15000"
     CDS             complement(4598..5086)
                     /locus_tag="VLA60_15000"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSF10716.1"
                     /translation="MNDEQPATVCASLIEGMQSLQLSTVGVDGIPHCSYTPYLHRAPS
                     SFYIFVSQLAAHTRHLLANRTVAIMIIADEQSTSQIFARPRVTYLCEAKRIAPNSPEY
                     ESVLDDYQERHGKMAGLLRQLPDFVLFHLHTKSGQFVMGFGKAYTLTGDDLSVFEHSR
                     TG"
     gene            complement(5292..>5577)
                     /locus_tag="VLA60_15005"
     CDS             complement(5292..>5577)
                     /locus_tag="VLA60_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10717.1"
                     /translation="RLVYFVTPKSCRDGKKFKERRPSQLGDRLAKHKIDDARKLTPEQ
                     QLLLTLELSNATAPLQRACSNKHEPSFFPALKQPWHRSFFDHSVWLHLDK"
BASE COUNT         1341 a         1352 c         1456 g         1428 t
ORIGIN      
        1 atccaagtcg aagtgtcagt tgggaggatt gaaagcgagt tccatctttg gtgatcgtga
       61 gtgtatggga ctctcgacca tagttgaagg ccggacggca ttcgagacga aaggtcatcg
      121 attttctaat agcttgcacg cggcggatca acccgtcccg aatgcagtca gacgtgcgaa
      181 ccccgaccgg cataaaatcg atcacccgcc ccactcctgc cggagtatgg aaccgggtca
      241 cgaggacatt ggtgtccggc caatagtatt gtttggattt gaccccgtcc ggaacgggag
      301 cgattttaaa cctgccccct ttggcatcat ccagaatagc gcagaacaca ctcggagagt
      361 cgaaataagg atagcagaac caatcgatgg acccgttcat gccgaccagt gcggtcgtct
      421 gcatatttcc gatcaggccg taattttcga tgggctgata gggcattgtt gaaaacgagt
      481 caatctcttg atacgagaga gtcggattgc tgaacgtctt tgtttacctt atcagaaaat
      541 gcagggtatg tccgcaaaaa gtgagccgaa ccccccgata ccacgcgagt gtatttatgg
      601 tgggcgaaat tcttattcag atggtgtacc atgggatccg gttgagcaca ggcgcagaga
      661 ataccatggg tatgacacga tcctatcccc cagtcgtgga aatttatgga gccgagtacg
      721 gctatgaacg gatactgaag gacgattttt tctccgtcaa tgttctctat cggaatcagg
      781 aaggcgaacg atatgtcctt aagctcagcg actttcgctt tgtgctgggc tggctgtttc
      841 gccccctgac gggttggata tccaggcggg aatatcgcat ctatcagatg gtgtctgatc
      901 tccccggcat tccagcctta gggccgcaat acgggaaacg aggatatttt catcgattca
      961 ttgaagggca cacgcttcat gaaatagaaa gactcatcca ggaacaattt aaggtggtgg
     1021 tgggggatcc ggcgatggct acgtatgcca caacgctggc tcctgatttt ttcgatcaat
     1081 tacggggcat tgttcagtcg gttcaccggc ggcgcatttt ctatgcggac ttaaataaac
     1141 ggggaaatat tatttgttca accgaaggaa agccctacct catcgatttt caaatttgcc
     1201 tgcctgttcc ggttcggcag ggttgggtag gcgcgctgac tgagaagatc ttccaacgat
     1261 tgattcgaga agatctttat catctgtata aacacaaaca caccttccaa ccgcagaact
     1321 tatccgagaa ggaactgcag ttggcgcaac gatcgcattt aaatacgcga taccatcgct
     1381 acctttggcg gccctacatt gccctgaagc gattgatcta tccgcatggg agcaatgaaa
     1441 ccatctggta taaatggaaa aaggaaaaag accaatcccc ccgaatgtcc tgattctcta
     1501 atcgatgtga gaggaagggg cttccctcat gccttatcat gaaatcttct ccctccggga
     1561 acaatgtcca tatcatgtgc agtcgttcgt atcatgacat tgccggtctg ttatgcgggt
     1621 tgtaaatgca gcattctttt aatttgctcc acaatcactt ctggcgcttg ggaaacgtca
     1681 atgaagggga catcgtgagg ttcttcaagc gcagtaaact gactggctaa caggtcggct
     1741 ttcataaagt ggtcggttcg tgactgcacg cgtgggtgga gcaacgcgta attgcctcgc
     1801 aaatacacca atgcgacttg agggtgtccc tcggtgatca acttgcgata ggtctgtttt
     1861 aaagcggaac acgtgatgac ggcgcgctgt gattggctta tcaggtcgtg aaccagttga
     1921 tggatcgcca agagccaggg aagccggtct tcatcactta acggaactcc accaagcatt
     1981 ttggacacat tgctgggtga atggaaatca tcgccttcgt aaaaagacca accaagcttt
     2041 ctggccagga gttgcccaac ggtcgttttt cctgaacccg agacgcccat aaggatcacg
     2101 atccatatgt cccggttctt tgcctctctc atagccaatc gttaggagcc catttcctcc
     2161 agaagtgaga cgaggtcatc agcatgctct tcctcgacag ccaaaatgcc ctccaacatg
     2221 cggcgggatg tggaatcttt agtgtttaga tagttgatca cttcccgata gctatcaatg
     2281 gcaatgcgct cggccaccag atcctccttg atcatgtcga ccagggaagt gccttccaca
     2341 tattcggaat gactgcgcat gagcattccc tcaggggaga gattcggttc gccgcccagt
     2401 tgaacaatac gctccgccag ttgatcggca tggccttgct cttcattggc atgttggagg
     2461 aattccgccg ccacgctctg agcatgaatg cctgacgcca tataatagtg ccgcttatac
     2521 ctgaggacac agacgatttc agttgccaga gcttcgttga gtagcttaat caccgtatcc
     2581 cggtcagccg cataaccagg cgtcactgcg cctttttcca tgtgttctct tgcgcgtttg
     2641 cgtaaggttt gtacgtctgt gagaaatggt tggtccatca ttcacgcctc ctgcttcgag
     2701 tggcttttct cgtcaataaa cagaatttca gccgaggaac cgtctttatt ctgatttccg
     2761 accaagtgcc tggactgcat ccattgaatg aaataattct ctcaatcctg tctgccacag
     2821 aacaatggcc aaactcaccc cattcatccc gctatttgtc caaaaagttg aagattcttg
     2881 gtagattggg tgtggtaaat tggttccaac ctgaggagcc gaagccgtct gatgcattga
     2941 ctcagaaagg acagccgggg ttcgactggg ggtttttctt gttcaaaggc catgggatag
     3001 gaaccgggca agccaacatt gaggtgtaaa cccgacgatt atgcaatctt atcgaggtga
     3061 ggaaggcgaa tgcctcgttc ttcgtcacgc aagagttgtc tctacgggag agccctgaag
     3121 cgtatcggta tcccgatacg cgggaaaatg gctgaggcaa gattctgctt tcgcctgcgg
     3181 cgtgaggata aaaatcggag gatcacccat catcttgcct atgggtttca tcggtaagat
     3241 gacaggagat gtatagaaaa actcggactg aatatcgcac gggattagct gacggccgga
     3301 aggtccagta tgacctcacg cggaccgtcg gccttgggtc aaaccggcag cgttgtgttt
     3361 cagaacaaat ctttatacat gctcaggctt tgattcagaa tattttgagc gacttttttc
     3421 tctaaaaatt cctccaccac cacggctttg tgttcaagag tcttgtactc ttcaaagaaa
     3481 tggcgcattt cggaaatgaa gtgctggggc aattccgaaa tatcttttat gtgactgaca
     3541 ctaggatcgc ctgccgcaac cgcgatgatt ttgtcatcgg cctctccgtt atcaagcatc
     3601 cgcatcaccc cgatgacttt ggccggcacg atacacatcg ggactacatc aatttgtgaa
     3661 ataatcaaaa tatccagggg gtctttatct tcacaatagg tttgcggaat gaacccgtag
     3721 ttcgcagggt aatagacgga agaatataac accctgtcga gtctcaacag gccactctct
     3781 ttgtctaatt catatttggc cctggtgcct tttggaattt caataatgcc attgacaaat
     3841 tcgggaacct tgtcgccagg ggtggcatga tgccaggggt tgaaaaaatg gttcttgggt
     3901 aatgtatcca tcatgtttat cctgataaga gggtggtgaa tgtgattatt tctctgaata
     3961 tgtgtcgcaa atcttgtttc atcagatgaa tggcgtgtta gttggtcccg ccatggaatt
     4021 tctttgccaa tcgttgctcc gtctcgtgcc tcccttgttc aacagacctt cgctgaacgg
     4081 gtctgctcag tgatgggggt cttcgcgccg tttctcgcgt gggtattccc tgatacccat
     4141 tcaagcctct ccctccgtgt gatttccgca cgcgagggag cacgcaggaa ggaaattcac
     4201 acgtattcga aatgtacgga ttgccgtccg atggagtccg ctatccttgt gagggggagg
     4261 atccaggagg agccgtggct ctagcaagag gaccaaagcc cgggctatca agcccgaggt
     4321 tgcaccatga cgatcgcttg tgtgatggag tgttccctgg aacagttatc gggaaggtgt
     4381 cttcagtcct tggcaaggat agcgaaccct tactctcctg taaagcatat atttacccac
     4441 aggcacgttg catatcagag acctcggggc ggttcaaaaa agttcgtcca gcaaggccgc
     4501 agtcattttt gcccgcagag cgtacttcca gtacgtgagc acgggaaaat gacgagaacg
     4561 ccgctggcgg cttttttcaa tagccccatt aatcacgcta tcccgtgcgg gaatgttcga
     4621 ataccgacaa atcgtctccg gtcagtgtgt atgctttgcc aaatcccatc acaaactgcc
     4681 cactcttcgt gtgcaggtga aacagaacaa agtccggcag ctgccgtaac aggccggcca
     4741 tcttgccgtg ccgttcctga tagtcgtcca gcacagactc gtattctgga ctgttgggtg
     4801 cgatccgttt ggcctcacac aggtacgtga cccttggacg ggcgaaaatc tgtgacgtcg
     4861 actgttcatc ggcaatgatc atgatggcga cagtccggtt cgccagcagg tgacgggtat
     4921 gtgcggccag ttgactgaca aagatgtaaa agctgctggg agccctgtgc aagtagggcg
     4981 tataactgca gtggggaatg ccgtccactc cgactgtgga gagttggaga ctctgcatac
     5041 cctctatgag actggcgcag accgtagccg gctgctcatc gttcatggga ttcaactacc
     5101 tccgtttaaa gggttccagg tggagagtcg ggaagcgaat gatgaccttc acgcttcccc
     5161 ctgatgtcag gttctatggc aaatacgcat agagaggaaa aactcaattg attgattgtc
     5221 tgcctcatgt agcaagggtt gagaaccgtt ctgccctgtt tttctggtgt ttcgatagta
     5281 cgggaacggg cctatttgtc aaggtgcagc cagacagaat ggtcgaaaaa ggacctgtgc
     5341 catggctgtt tcaaggcggg aaagaatgac ggttcatgct tgttggaaca cgcgcgttgg
     5401 agaggggcag tggcattgga cagttcaagg gtaaggagta gctgctgttc aggagtgagt
     5461 tttcgggcgt cgtcaatctt gtgtttggcg agcctatcac caagttgact gggacggcgt
     5521 tctttgaact tttttccgtc cctacacgac ttgggtgtga caaaatagac caatctc
//
LOCUS       DATIAL010000489         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1054537, whole genome shotgun sequence.
ACCESSION   DATIAL010000489 DATIAL010000000
VERSION     DATIAL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054537"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VLA60_15010"
     CDS             complement(<1..1266)
                     /locus_tag="VLA60_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HSF10718.1"
                     /translation="MKPRSSAFSSRPHPHLYEINTWVWLHDLSQKMNRTIRLGDVPDQ
                     ELDEFQSKGFDCLWLMGLWERSPLGRRIARQHADLQKEYAKALPDWQEYEVVGSPYAI
                     RAYQPDPELGSWEQLKDVLGKLHDRGIKLILDFVPNHTALDHEWTTRHPEYYVQGTSR
                     DVTNFPSQFFPIETSQGIRYLAHGKDPNCPSWTDTAQLDYFNPDTRKALIRELQQIAN
                     YCDGVRCDMAMLVLNEVFAQTWENHVKSDVCPSGEFWSEAISLLPDRMWIAEVYWNRE
                     WELQQLGFHFTYDKRLYDRLRHSTPHEVALHLQADTIFQSKLVRFLENHDEPRSAVAF
                     GNVRLPAVGVLMATLPGMRLYHQGQLTGKRIRIPVQLCRAQDEPPDEATVAFYDRILA
                     ITNEDVFHAGKWAMLTARSAGDDSFNNVVA"
     gene            complement(1666..2088)
                     /gene="rnk"
                     /locus_tag="VLA60_15015"
     CDS             complement(1666..2088)
                     /gene="rnk"
                     /locus_tag="VLA60_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HSF10719.1"
                     /translation="MERRDIYITEFDLNRLTELLGVWQTFKGSKSTSIHLESLMEELD
                     RAHIVLPKDIPADVVTMNSRVRLSDMSKGEELVYTLVFPRDADAAAGKISILAPVGTA
                     ILGYRVGDSIEWPVPIGTRKLKIEEILYQPESAGDFHL"
     gene            complement(2250..>2908)
                     /locus_tag="VLA60_15020"
     CDS             complement(2250..>2908)
                     /locus_tag="VLA60_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HSF10720.1"
                     /translation="CLRSRNLVNVIVAGKQPGLQFLSMDAAIKHCTAGIGIWEWASND
                     KGGEPDVVMVCAGDIPTLETLAAVDLLRKQVPDLKVRVVNVVNLMKLQPQSEHPHGLS
                     DKEFDTLFTTDKPVIFAFHGYPWLIHRLTYRRTNHKNLHVRGYKEEGTTTTPFDMVVL
                     NDLDRYHLVADVIDRVPKLGYLAAYAKQAIRDKKIDHKTYIAKYGDDMPEIKDWKWPY
                     "
BASE COUNT          776 a          762 c          665 g          705 t
ORIGIN      
        1 cgcgacgaca ttattaaacg aatcgtcgcc ggcagaacgc gcagtgagca tggcccattt
       61 cccggcgtga aacacatctt cattggtgat cgccagaatt cggtcataaa aggcgacggt
      121 cgcttcatca gggggttcgt cctgggctcg acagagttga accggaatcc ggatacgttt
      181 tccagtgagt tggccttgat gatacaaacg catgcccggc aatgtggcca tcaggacccc
      241 aaccgcaggg agacggacat taccaaatgc caccgcactt ctcggctcgt cgtgattctc
      301 caaaaaccgc acaagtttgc tttgaaagat cgtatcagcc tgaagatgca gagcgacctc
      361 atgtggggtg gaatggcgaa gccggtcata caatcgtttg tcgtaggtaa agtgaaagcc
      421 aagctgttga agctcccatt cccgattcca atagacctct gcaatccaca tacgatccgg
      481 cagaagagag atggcttccg accagaactc accggaaggg cagacatcgc tcttcacatg
      541 attttcccac gtctgtgcaa acacctcatt gagcaccaac atcgccatat cacagcgcac
      601 gccatcacaa taattggcaa tttgttgcaa ttcgcgaatc agcgccttac gcgtatcagg
      661 attgaagtag tctaattgcg cggtatccgt ccatgaaggg caattgggat ccttgccatg
      721 tgccagatat cgaatgcctt gtgatgtttc gatgggaaaa aactgcgagg ggaagtttgt
      781 gacatccctg gaggttcctt gaacataata ctccggatgt ctggtggtcc actcatgatc
      841 caatgcggtg tgattgggca caaaatccag aatcaatttg atgccgcggt catggagttt
      901 tcccaatacg tccttcagtt gctcccagga ccccagctca ggatcaggct gatacgcccg
      961 aatggcataa ggggaaccca cgacctcgta ttcctgccaa tcaggaagcg ctttggcgta
     1021 ttccttctgt aaatcagcat gttgtcttgc aatccgccgg ccaagaggac tgcgctccca
     1081 taggcccatg agccacagac aatcaaatcc ctttgactga aactcatcca attcctgatc
     1141 aggcacgtca ccaaggcgaa tcgttcggtt cattttttga gagaggtcat gcaaccacac
     1201 ccaagtattg atttcataga gatgcgggtg cgggcgagat gaaaaagccg aagatcgcgg
     1261 tttcataaga gtccatcccc tccgtctcat tcatgttgac tgagggattc tacacgatca
     1321 gggatttgat cgcggaaaaa gtgtttcctc tcataacgat atgcgaggac aggcacgaaa
     1381 tccaacagat aaaccggaca gtccctggaa taaaaaatcc attgtgaaca gacgattggg
     1441 aggcaatgat cgcttcatag atgagacacc ttccttaata ataaccaaag catatttccc
     1501 ggaagatcag ctcgatagca aagatgttat tggtatccca tctttcgtga aagccgacag
     1561 catgaaccct aaagcgttgc tcccggccgg ttcactaatc gtttttacgt ctttccctta
     1621 aaaattccca tactgttcat gcgaactgat agagcccgga gaaatttaca ggtgaaaatc
     1681 tcccgcagac tcaggttgat agaggatctc ttcaattttc aacttccttg tccctatagg
     1741 gaccggccat tcaatactgt ccccaaccct atacccgaga atggccgttc caaccggggc
     1801 aagaatagaa atctttcctg ccgcagcatc cgcatcacgc ggaaagacca acgtatatac
     1861 cagctcttcc cctttactca tgtctgatag ccggacacgt gagttcatcg taacgacatc
     1921 agccggaata tcctttggga gaacgatatg cgcccgatcc agttcttcca ttaagctttc
     1981 cagatgaatg ctggtactct tgctaccttt gaatgtttgc cagaccccta atagctcagt
     2041 gagtcgattc aggtcgaatt ccgtaatata aatgtctcgg cgctccatcg ggtgactcct
     2101 taaaagaaaa ctcagggtaa aaggataaaa gaaaaaacct ctggcatcct catgaaagag
     2161 aataccccac taatctatat ggctgaatta tgttaacgga gcaggccttg aatcgcagca
     2221 cccctagtga tccaaatggg gcgtgaaaac taatacggcc atttccaatc tttaatttcc
     2281 ggcatatcgt cgccgtactt ggcgatgtac gttttatgat cgatcttttt atcgcgaatc
     2341 gcctgtttgg cataggccgc aagatatccc agtttcggca cacgatcaat cacatccgcc
     2401 accagatgat accggtcaag gtcgttaaga acaaccatat caaacggggt ggtcgtcgtg
     2461 ccttcttctt tatagcctcg aacgtgaaga tttttatgat tcgttctccg gtacgtcaaa
     2521 cgatggatca accagggata tccatgaaaa gcaaagatga cgggtttatc cgtcgtaaag
     2581 agcgtatcga actccttgtc ggacaacccg tgcggatgct cgctctgcgg ttgaagcttc
     2641 atgagattca ccacattcac cactcgaact ttcagatcgg ggacctgctt gcggagcaga
     2701 tccaccgcgg ccaaggtctc cagggtggga atatctcccg cacagaccat gaccacatca
     2761 ggttctccgc ctttgtcgtt actggcccat tcccaaatcc cgataccggc agtacaatgc
     2821 ttgatggccg catccatact gagaaattgc agacccggtt gctttccagc cacaatgaca
     2881 ttgacaagat ttcgacttcg aagacatt
//
LOCUS       DATIAL010000490         6029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_304931, whole genome shotgun sequence.
ACCESSION   DATIAL010000490 DATIAL010000000
VERSION     DATIAL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304931"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            700..1599
                     /locus_tag="VLA60_15025"
     CDS             700..1599
                     /locus_tag="VLA60_15025"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10721.1"
                     /translation="MEHVVGARIHQLRTIDPGEIRLVQLALPEEVEIAGKLYALRDTM
                     RPGTAFLAKPWGDRTGKYRRRMYTRSNASFSEPRLLETFINYTHLDQSDTSSWWQSEM
                     ATDWFQKGKTVEVRLQMDEEQGGLLVYENTKVCKATNLRLEDDGIWEGMRMVCVAFGT
                     GITPFLSYARYLKAKNFGRKGGRVGAYLTLVASVRHEGQLMLHEEMVALEKQFPEHFR
                     YQPVLTRSWPTAWEYPKGRIQRVVHSESGDEQIDISPFQAIVPNIAQSHLRICGSVTA
                     CRQLTLGLEQGGMVPLTLRTESW"
     gene            complement(1646..1930)
                     /locus_tag="VLA60_15030"
     CDS             complement(1646..1930)
                     /locus_tag="VLA60_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10722.1"
                     /translation="MKKSAQPDQAIGKSRKTSPKKTVTKKASSDKYPRCSRCGSRTVH
                     LEQLEQELLVTLSLHCLICGHYTFLGRPVIRLLRRPNVTIPDSITRPAGE"
     gene            complement(2128..2553)
                     /locus_tag="VLA60_15035"
     CDS             complement(2128..2553)
                     /locus_tag="VLA60_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10723.1"
                     /translation="MLLIGLLSACTSHSKTPSPSSRSSLPATTGKATTPALENQLLAT
                     VKHAEGLGPGNPLLLSSLYSLATYYEDRKEYDKSAAQYERALKLKETANGPNHPDVAA
                     ILQRYARMLQAANRPSEAANLRLRSEAILAHPSPPTSSQ"
     gene            2755..3843
                     /locus_tag="VLA60_15040"
     CDS             2755..3843
                     /locus_tag="VLA60_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10724.1"
                     /translation="MTVLWCSISSHGFGHAAQLMPILNELGGAVDDLHVILRTQVPAD
                     FFQRHLRVSWELQDVQQDVGCVQRGPLDIDVEETWKAYAKFHTQWESKVEEEAQAIRL
                     SNARLVISNISPLAIAGAAQVKCPVVGIASLSWDRVLEPFAQADSGIHRSILETIRTS
                     YGLADSLIRLHPGIEMPAFGSTVDVGPSAPMRKPNGYDLRKALGVEEHELIVLIAFGG
                     VPLNRLPLEQMSAMAGFHFLVSELPSFSSYASVHRVEDLAMPFGEISQQADIIMTKPG
                     YGTVIAAVQDKTALVYVRRHNFIDEQSLVDYIHRHGRGMELSRDDFESGNWEPTLRAV
                     LTVAVPSEAHPSPGTDDVVRQLKTYLSC"
     gene            complement(3867..4346)
                     /locus_tag="VLA60_15045"
     CDS             complement(3867..4346)
                     /locus_tag="VLA60_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10725.1"
                     /translation="MFVGTDITHQPAFPLEASREGIASAPSTSRLLRPLSSRLPSSTF
                     TTSPEPVSIAMLLSQPASYHQQLVAVRGVVTQPEMHLNDSELAIRFVFRLAEGEQSII
                     VFGQHDRTQGSPSISLDLSVEVIGVFWKERELNASRVLNTIEALTVSPYPSLLPDSA"
     gene            4506..5183
                     /locus_tag="VLA60_15050"
     CDS             4506..5183
                     /locus_tag="VLA60_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HSF10726.1"
                     /translation="MPVVDYYAVLGVVLQASQDEIKKAYRALALQYHPDRNRGNRQAE
                     QKIREVNAAYEILGDPDARKTYDRMRLGYVEPMVHRRDRDPEPEPEESISPSVVLQRM
                     EETLREESRKQLFMVLIRNTQRIKEELGIIRERVIRAQGYDTFLEKIVIQRGREVLDE
                     LVSVELKQRRERLVDVAVEMVCSAVPGSFRGEEQMDQVRRSLGQAYQEGWIQGYEQAC
                     ELLYERR"
     gene            complement(5323..5577)
                     /locus_tag="VLA60_15055"
     CDS             complement(5323..5577)
                     /locus_tag="VLA60_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10727.1"
                     /translation="MTLPEVGAIQFMQPASSGDHSRKQVTAVSSDEESGKAKSHRERG
                     APSPIQDQVTLSKEAQALSTSRHHASKGNSFQQPSSPFDR"
BASE COUNT         1580 a         1285 c         1664 g         1500 t
ORIGIN      
        1 ttgtgtgagg ccatgttaca aggccaaatt cagaccggca agatgaggag gagagaatag
       61 aaaaggactg accttaatat acaagaacac ggtaaaaact ctagaatgtt ttctgatgtc
      121 tggccaatgc gtggaccgag cattccatcc gcttggaagg agcttaatgg aatgactctg
      181 cccttaagcg gcaatgaaat tacaatgtgg ttggcgggcg gatttcttct ggatgagtat
      241 gaagttcttg aatgagtgaa tccgctaact tttcagcaga aatgacatag ttctgtgagt
      301 caatggcttt ttgaaaatgt gcgattttgt cctgtcgaac atccggaacc tgagacattt
      361 gttcgaggta aaaagaaacc tctcggctag gagtggaggg ggatgttctg tcatctttgg
      421 cagagtttgg agctaaagca gaaacctcgt ggctaacatc cggaaatggc aaggccgttt
      481 tcttaggagg aaatgatggg gcatctcctg gtggtgttgg tgggatggga gaaagttgac
      541 tcattggttg tcaaagggga acccaggatg aggtgaaatc ttaaataaat atgaatagaa
      601 gaatcaaatg gcattgtcgt cccttccctt ttggccgtgt aagttcgatg aaatcactcg
      661 tcaccttatg agcggttctt agataggatt gagatgatca tggaacatgt tgtgggcgca
      721 cgtattcatc aattacggac cattgatcca ggagaaatcc ggttggtcca gttagcactt
      781 cctgaggagg ttgagatagc aggaaaactc tatgccctcc gagataccat gcgtccaggg
      841 acggcctttc tcgcaaaacc ctggggagac cggacgggca agtatcgaag acggatgtat
      901 acccggtcaa atgcttcctt tagcgagcca cgcttgttag agacgttcat caattatacc
      961 catctcgacc agtctgatac ctccagttgg tggcaatcgg aaatggccac cgactggttt
     1021 cagaagggaa aaactgtaga agttcgtctc caaatggatg aagagcaggg gggcctgttg
     1081 gtgtatgaaa ataccaaggt atgtaaggct acgaatcttc gcttggaaga cgatgggatc
     1141 tgggagggaa tgcgaatggt ctgtgtggcc ttcggcacgg gcattacgcc attcttatcg
     1201 tatgcccgat atttaaaagc caaaaatttt ggtcgaaagg gagggagagt tggagcctat
     1261 ctgacgttgg tggctagtgt gcgccatgag gggcagttaa tgcttcatga ggagatggtc
     1321 gcccttgaga aacaattccc cgaacatttt cgatatcaac cagtgctaac tcgatcctgg
     1381 ccgactgcct gggagtatcc gaaaggtcga atccagcgag tggtacactc cgagtcagga
     1441 gacgagcaaa tcgacatttc accatttcaa gctattgtgc cgaatatagc ccaatcgcat
     1501 ctacgcatat gtgggagcgt cacggcctgc cgacagctca ctctcggact tgaacaaggc
     1561 gggatggttc cgctgacgct gcgcacggag tcctggtaga ggaaatccat gctgaaaggc
     1621 agaggaattt ggtgtgaatg atttcctatt cccctgcggg ccgtgtaatg gaatctggaa
     1681 tcgtgacatt tggacggcgt aataaccgga tcacggggcg accaaggaag gtgtaatgtc
     1741 cacagatcag acagtgcaag ctgagtgtga caagaagttc ctgctccaac tgttctaagt
     1801 gtacggttcg cgacccacaa cgagaacaac gaggatactt gtcggaggag gcctttttgg
     1861 tcacggtctt tttgggagag gtcttgcgtg acttgccgat tgcttggtcc ggctgtgctg
     1921 actttttcat gaatgaatcc ttggtcaagg taaaagaaga actcatctgg ttataaccaa
     1981 ttcctgtgcc aaaatttcag aggaaaaagt gacaggaaag tcgaaaaacg gcctataaat
     2041 aggaggaagg aagccgacac tgggtaaaat tttcccgagg aacagggtca aaaatgctgt
     2101 gctggagagc cgttgtccag ctaaagttta ttgactggaa gtgggaggtg agggatgagc
     2161 gaggatggcc tcagaacgaa ggcggagatt ggctgcttcc gatggtcgat tagctgcttg
     2221 caacattcga gcatatcgtt gaaggatggc cgccacgtct ggatggttcg ggccattcgc
     2281 ggtttccttt aatttgaggg cccgttcata ttgcgcagcg gatttgtcat attctttccg
     2341 atcctcgtaa taggtggcca aactatacag actacttaaa agcaggggat ttcccggtcc
     2401 taacccttca gcatgtttaa ccgtggccaa taactggttt tctaacgcag gggtggttgc
     2461 ctttccggtt gttgccggaa gcgaagaccg agaagaaggc gagggcgttt ttgaatggga
     2521 ggtacatgcg ctgagtagtc cgattaaaag catgatggtc cacatcatcc taaaaataga
     2581 tcgtatattc cggttcatag aactacaccc taaccatggc atctgcaaga aagccccgaa
     2641 tagaaagtca gaagtgattc tatgaactct gagcctgttc tcgcaagtga actccccggt
     2701 ttatgattaa tcaaatacca attcattgtg ctttttaatg gagaagaata cacgatgacg
     2761 gtcctctggt gttcgatttc atcccatggc tttgggcatg ccgcgcaatt aatgccaatc
     2821 ttgaatgaat taggtggggc ggtggatgac ctgcatgtga ttctacgcac ccaggtacct
     2881 gccgatttct ttcaacgtca tctccgcgtg agttgggagt tgcaggatgt ccagcaagac
     2941 gttggatgtg tccagcgagg gcccttggat attgatgtgg aggaaacatg gaaggcctac
     3001 gccaaatttc acacacagtg ggagtccaag gtggaggagg aagctcaggc catccgattg
     3061 tccaatgcgc gtctggtcat ttccaatatt tcgcctcttg ccatagctgg cgcagctcaa
     3121 gtgaagtgcc cggtcgtggg aattgcctcg ctatcctggg atcgggtgtt agagccattt
     3181 gcccaggccg attccggtat tcatcgttcg attctcgaaa ccattcgtac cagctatggg
     3241 ttggcagact ctctcatccg ccttcatccg ggtatcgaga tgccggcttt tggttcgacg
     3301 gttgatgtgg gaccttcggc tcccatgagg aagccgaatg gctatgatct gcgaaaagcc
     3361 ttaggggtgg aagagcatga attgatagtc ctcattgcct ttgggggtgt gcccctcaat
     3421 cgtcttccgc ttgagcagat gtccgcaatg gccgggttcc actttttagt aagcgaactc
     3481 ccatcttttt cttcttatgc ctcggtgcat cgtgtcgaag atcttgcgat gccattcggg
     3541 gaaatcagtc aacaggcgga tatcatcatg accaagccgg ggtatggtac cgtgatcgct
     3601 gcggtccagg ataagacggc attggtctac gtccgtcgac ataatttcat tgacgaacag
     3661 agtttggtgg attatatcca tcggcacggg cgagggatgg agttatcccg tgatgacttt
     3721 gaatcgggca attgggaacc gactcttcgt gctgtactta cagtagcggt tccgtctgag
     3781 gcgcatccgt caccgggcac ggatgacgtt gttcgtcagt taaaaacata tttgtcgtgt
     3841 tgaagggaaa cgggccgggg atcatttcaa gcactatccg gtaacagaga gggataggga
     3901 gagaccgtta gtgcctcaat cgtattcaaa acacgtgatg cgttcaattc tcgttctttc
     3961 caaaagactc cgatcacttc cacggaaaga tccagtgaaa ttgacggtga gccttgcgtg
     4021 cgatcgtgtt gaccaaaaac gataatggat tgttcaccct cggccaagcg aaagacaaag
     4081 cgtatggcta actctgagtc attaaggtgc atttccggtt gagtgaccac tccacgaacg
     4141 gccacgagtt gttgatgata ggaagctggt tgactcagca gcatggcaat ggagacgggt
     4201 tcgggtgatg tggtaaaagt ggaggagggg aggcgagatg aaagtggccg aaggagcctg
     4261 gatgtggacg gtgcagaggc aattccctcc cgggaagctt ccaagggaaa tgccggttga
     4321 tgcgtaatgt ccgtcccaac gaacatgaga gcattccatg ctccaatcaa aagaactttt
     4381 gaaaaaatgg tcatgaaaga aaagctgttt tttagaaata atatgcaggg acaagtgaac
     4441 gtggatattc gcaaaagctg tccaaataat caggtgaaga attttcttct gtaaggaaca
     4501 tgtcaatgcc tgtcgtcgat tattatgcgg ttctcggtgt tgttcttcaa gcatcgcaag
     4561 atgagataaa aaaagcctat cgggcattgg cccttcaata tcatcctgac cgcaatcgag
     4621 gaaatcgtca ggctgaacaa aaaattcgtg aagtcaatgc agcctatgaa atcttgggtg
     4681 atccggatgc cagaaaaacg tatgatcgaa tgcgtttggg ttatgtcgag ccgatggtgc
     4741 accgacgaga tagggatccg gaacccgagc cggaagaatc catttctccg tcagtggtcc
     4801 tgcaacgaat ggaagaaacc cttcgagagg aatcccgcaa acaactgttt atggtcttga
     4861 tacgcaatac acaaagaatc aaagaagaat taggcattat tcgggaacgg gtcattcgtg
     4921 cacagggata tgataccttt ctggagaaaa ttgtcatcca gcgaggccgg gaagtgttag
     4981 atgaactcgt gtccgtggag ctcaaacagc gccgagagcg actcgtagat gtagccgtgg
     5041 agatggtgtg ctcggccgtt ccaggttcgt ttcgaggcga agagcaaatg gatcaggttc
     5101 gacgaagttt aggacaggcg tatcaggaag gatggatcca aggatacgaa caagcttgtg
     5161 aactgctcta cgagcgccgg taatcaggac ggatacattg agaaaagcac aaatggaagt
     5221 ggataaggga aatgatcaac aatgaggagg caagccttgc tcagaatccg atgccagtaa
     5281 atgggatgcc ccttttgccg gattatcgcc gtttcacaac cctcagcgat caaagggaga
     5341 agagggttgc tggaaggaat tgccttttga ggcgtgatgc cttgatgtgg acaatgcctg
     5401 agcctccttc gacaaggtca cctggtcctg gatgggagag ggggcacctc gttcccgatg
     5461 gcttttggct ttgccactct cctcatcgga gctcacggcg gtcacctgtt ttcgtgaatg
     5521 atcccccgat gatgcgggtt gcataaattg aatagctcct acttccggta atgtcatggt
     5581 cagtactcct ttgctcttct gaatgagttc aagactccca ttacaatctc tgtatcggta
     5641 tgtgcccata atgcttgcgg cgcgggaatg ggtggattta atgaaatgac atagaatgtg
     5701 aactatttgc caaaaagggc aaggaactca ttgggatgct ccccacacca ataacgagcg
     5761 aatgggctat agaaaaacaa cagggatgtt aaaacaacgg tgcgactgga ctacctttga
     5821 agtggagcga tagaaaggaa ggatcagttt aattgatcgt gtgcttccct ttcatgaaat
     5881 ccacgagcct ctcactacca ccaaattatt gtgtttgtag aaaaaggggt gtttgcattt
     5941 agcaggaatt tttccaaacc aactgatcca cccccctcgt ttcctgacag attcgctaaa
     6001 atacttgggg tatcagaggt ccttgaaat
//
LOCUS       DATIAL010000491        10067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_840944, whole genome shotgun sequence.
ACCESSION   DATIAL010000491 DATIAL010000000
VERSION     DATIAL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10067
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840944"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..338
                     /locus_tag="VLA60_15060"
     CDS             156..338
                     /locus_tag="VLA60_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10728.1"
                     /translation="MTSYAKRILLVACFSGSLFGALGCEQEGPAERAGERVDESMEKA
                     GEKMEQAGENIQDSAN"
     gene            814..1206
                     /locus_tag="VLA60_15065"
     CDS             814..1206
                     /locus_tag="VLA60_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10729.1"
                     /translation="MNTPKHDMKWFLNVKFMMTFAVLMGVPGFAFGSDLAGHVPDHDR
                     SATTQKQEGHQLDKGHRLIEGVIQEVNENTIRVDAGEAGEIAPRYLNLSNSKGKGDFK
                     IGDMVQIEVNAQNKVVNFQLLHKDGARH"
     gene            1333..1497
                     /locus_tag="VLA60_15070"
     CDS             1333..1497
                     /locus_tag="VLA60_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HSF10730.1"
                     /translation="MLSWAITFLVIALIAGVVGLTGVAGTATNIAWVLFVVFLIIFAL
                     SFITGKRPPA"
     gene            1700..2059
                     /locus_tag="VLA60_15075"
     CDS             1700..2059
                     /locus_tag="VLA60_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10731.1"
                     /translation="MQFLHFQEKDFALCIPEHRQDVTSVVEKHAPEVIVLDMSIADPN
                     GPTLLKDLRATGYGGKIIVISGPSNRPLMVNMHHQKVDQVMRRTPTDPIGPFMDQLES
                     IIRVMFREEISRKPYPH"
     gene            2261..3121
                     /locus_tag="VLA60_15080"
     CDS             2261..3121
                     /locus_tag="VLA60_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HSF10732.1"
                     /translation="MTRPIWKGHITFGLVTIPVVVHSAEKQFDLHFRLLDSRNQARVR
                     YERVNEETGEEVPWQDIVKGFEYEEGRYVLLNDADFEKVAIEVNKSVEIENFVDGKDI
                     GYEYFDKPYYLVPDKKGEKVYVLLREVLRRSGKIGIAKVVIRTRQYLAALIPQGHVLM
                     LELLRFHQEIRNVKDLDVPEASVKSYKISEKELTLAQQLVESMSTKWEPQRYHDDYRD
                     ALLKWIEKKSKSAQSRTSVPHVAKEEKSGSGKVVDIMSLLKKSVQKAQPSGRAKKRKS
                     ASPRKSRKAS"
     gene            3226..5844
                     /gene="ligD"
                     /locus_tag="VLA60_15085"
     CDS             3226..5844
                     /gene="ligD"
                     /locus_tag="VLA60_15085"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HSF10733.1"
                     /translation="MGLKEYQGKRQFSKTPEPRGTTFRTNRSSFVVHKHAASRLHYDF
                     RLELDGVLKSWAVPKGPSLFPSLKRLAVQVEDHPVEYGSFEGTIPKEEYGGGTVMVWD
                     RGQWVPQGNPQHSYNRGRLKFLLKGQKLKGGWSLVRMGGAKAKEEKNWLLIKEKDSAA
                     LQGKHSDIIDMAPASVATGRSLEKIAELNQEESRKNPASQRKSKNSSTKSHSSRTPSR
                     KNRNSGTSRVPDPSDLPSAVSSSQPATFHPQLATLVKTSPEGEDWLHEIKFDGYRIVS
                     IVKNGRIRLLTRNGKDWTKKFPDIAGAMKALPVQQGILDGEVVVLRSNGTTDFQALQH
                     VLKGEASDPLVYFVFDLPYCEGYNLCRTPLIKRKELLWELIKSLPEGAAVHVRYSDHI
                     QGHGKRAFEQACRQALEGIISKYAMSPYRQARSRQWVKVKCHHRQELVIGGYTNPSGS
                     RKFFGALLLGYYDKQGHLQYAGRVGTGFTHQRLSRIYALLAKRAQSHPPFVRLPSGRD
                     IRDVHWVHPELVAEVEFFEWTRDGMLRHPSFVGLREDKPAKEIIKETPHPVNNISEQL
                     KPSSRRSHASPEISMERPELALTHPEKILYPQQGITKRNLAVFYQQIAKWVLPHVAGR
                     PLTLVRCPQGSQRKCFYQKHASGSLPDSVHCILIREKETKTKNTYLVIDDVKGLIALV
                     QMGVLEIHPWGCRKDRTDRPDRLVLDLDPGPGVKWEQLVEGAHMLKSRLSGDGIESFV
                     KTSGGKGLHVVAPLTRRATWDQLKQYTRKLAMETAQAHPSGFVATMSKAKRKGKIFID
                     YLRNSFGATSIATYGTRALPGAPVSTPVSWDELFSISGSQVFTLSTLPHRLHTLRADP
                     WKGFFDLRQTLSRSRV"
     gene            complement(5898..6203)
                     /locus_tag="VLA60_15090"
     CDS             complement(5898..6203)
                     /locus_tag="VLA60_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10734.1"
                     /translation="MPRGDKSKYTGKQKRQAEKIEEGYEKRGVSEEEAERRAWATVNK
                     MTGGGKKPGGSGRGKGMNTAPAKKGGEKGGKASASRSASAKSASARKAAETRARKKK"
     gene            complement(6290..7261)
                     /locus_tag="VLA60_15095"
     CDS             complement(6290..7261)
                     /locus_tag="VLA60_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HSF10735.1"
                     /translation="MSRDTAKKMSGTRGRNAAQPSQIPNTGWWDILLRVKDQISQDNV
                     SIVAAGVAFYAVLALFPALAAIVSLYGLFTEASDVQQQITSLSSFLPQDTQTLIQEQL
                     TTISTSGQSALSFGAIGGLLFAIWSASKGMSAMITSLNIAYNEEEERSFIKVTALALG
                     LTVGGLIFVILTLFLITLLPAVLEALGFGQKMQTVLSLSRWPLLAMIVMAGLAILYRY
                     APCRNYPKWQWVSWGAGIATLMWIVGSSAFAIYANDYSSYNQTYGSLGAAVILLMWFW
                     LTAFIVMVGAEINSEMERQTRVDTTEGKPEPMGQREAYSADTLGQQQ"
     gene            complement(7406..8284)
                     /locus_tag="VLA60_15100"
     CDS             complement(7406..8284)
                     /locus_tag="VLA60_15100"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HSF10736.1"
                     /translation="MDDQRTQLNAQESKKIEDPARLREQIRVTRAELDGTVHTLQHRL
                     SPDTIKEQVKTAAIDKVETAKENARVKVNQWQSLLVERVLNNPLPAAFIGVGLLWMMK
                     QAGNSSSARSRFGRDRYGDDYSLDPYEEWPDYAPEIKAGRIPRQRSRRGRSLDAMKTR
                     AQASGQQAKEQISEWPNQAQESLEDWKEKASHRSDEFTERTREAGKRMKDELFRYMQE
                     NPLTIGAITFAIGTAIGLSLPRSDKEDQWMGETRDRLLEEAKATAKEIMPKAKEAAAE
                     AQRVATESIKEQASMN"
     gene            complement(8260..8652)
                     /locus_tag="VLA60_15105"
     CDS             complement(8260..8652)
                     /locus_tag="VLA60_15105"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HSF10737.1"
                     /translation="MSLYQEERPLSDLFRDLLNETKILIQQEMQLLKLEMSQKAKQAG
                     KDVGFIAVGGALAYAGLLVLLCAATFALALIIPAWASALIVGLAVVGIGYALIQKGIS
                     DLKTMDPAPQKTIDSLKETKQWTTNVLN"
     gene            complement(8699..9214)
                     /locus_tag="VLA60_15110"
     CDS             complement(8699..9214)
                     /locus_tag="VLA60_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10738.1"
                     /translation="MANDMREKAKEIGKQVQEKASEVGEQVRAQGSELANQAQGKVKS
                     TLETQKQKGVGELSGLVEAVRQTSQHLREQHKESVAHYAERAAEQMDRMVQYFDSRDV
                     GELLNETEMFARRHPEAFLGGAFMLGLVAGRFLKSSRVRRIDESSSYGRQESFRSEES
                     TATTTPAAPTM"
     gene            complement(9600..>10067)
                     /locus_tag="VLA60_15115"
     CDS             complement(9600..>10067)
                     /locus_tag="VLA60_15115"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10739.1"
                     /translation="TGVEYLIPIYKDVNTYPYIKEEGITGNADEKSLHELHGQIWPVV
                     KADADHGIEEARIKCEEYLGTSKASHDLKTVVLAAHEGKIDTLFVARNIQQWGRIDVM
                     QNRVETEPTKSAESLEILDFAVVQTLLKKGKVYALSQEKMPGRASLAAIFRYE"
BASE COUNT         2563 a         2417 c         2507 g         2580 t
ORIGIN      
        1 ggacagggtg atgaaggcgg ttcattccag agtggaatcg acggtggagg aggtgaaaca
       61 gaaaaccgga caagttgcgg aaaaggtgtt accggaaggt acacgaaatc ttctttaaac
      121 gaaaaaatgc tatgcaatcg gacgaaagga aacttatgac ttcttatgca aaaagaattt
      181 tattggtcgc gtgcttttca ggatctttat tcggtgccct gggttgtgaa caagaaggtc
      241 ctgcggaacg agcaggcgaa agagtcgatg aatccatgga gaaagcggga gaaaagatgg
      301 aacaggccgg agaaaatata caggattcgg ccaattaaga atctcctact gacagatgag
      361 gaaattattg tctccatttt tcctcattta aaagaagttt tgtccattgt atgaagcgtc
      421 ttaaaagggc aacactgaat gatatatcga tcattgtgtt gagaaaaatg ggattttgta
      481 atggaatttc tggagttaat tcctataacc ctgacggaaa ggagttatga tggccaccgc
      541 caatgctaaa aaatgtgctc accctggctg ttcatgcgaa gcccctccag ggaaggagtt
      601 ttgttccgaa aattgctcat cttcccaacc ttccaaaggc ggaaaatgtc agtgcggaca
      661 ttccggatgt gggcattgag gctaacagca cgaatgctca aggaacgaat tccgggaatc
      721 aatcaagaaa cccaaatgat accggttgat gtagtgataa tcctgggatg cttttgggat
      781 ccagcgggaa aatcaattac gaaggaggcg aacatgaata cccccaagca cgatatgaag
      841 tggtttctga atgtaaaatt tatgatgaca tttgctgttc tcatgggggt tcccggattc
      901 gcttttggta gcgatctcgc aggccatgtc cctgatcatg accgttcggc aacaactcaa
      961 aaacaggagg gtcatcaatt agataaaggt catcgcttaa ttgagggagt gatccaggaa
     1021 gtgaacgaaa atacgatacg ggttgatgcg ggagaggctg gtgagatagc tcctcggtat
     1081 ctgaatttga gtaattccaa ggggaaagga gatttcaaaa ttggagatat ggttcagatt
     1141 gaagttaacg cccaaaataa ggtggtaaat tttcaactgc ttcacaaaga tggagccagg
     1201 cattaaattt gccataatgg atttggagct ggaaacgggg aacgcacaca ccatcaacag
     1261 atggcatata aggcattccc gatcaacaaa ccattccgcc ctcttcggaa gggaataaaa
     1321 ggagaattac gtatgttaag ttgggccatt acatttctag tcattgctct catcgcagga
     1381 gtggtcggct taactggagt agccggtaca gcgaccaata ttgcctgggt gttatttgtc
     1441 gtatttctga taatttttgc cttaagtttt attacgggaa aaagacctcc ggcctaggtg
     1501 gaggattgag ggactacacc cacccgtcat agataggaaa ggtaggaaac gcctaccatg
     1561 aggtaaatga gggtttgctg ttgcggcacg actgcattga gatggagaaa aattcaactt
     1621 aagtaccagt gagacgcatg ccagggaatt gtggttttta acatgatcat ggtcgttacc
     1681 cctgacgagg tctttcgaag tgcaatttct ccattttcag gaaaaggact ttgctctttg
     1741 tataccggaa cataggcagg atgtgacttc agtggtggag aaacatgccc cggaagtcat
     1801 tgtcctggat atgtctattg ccgatcccaa tgggccaacc cttctgaaag atctgcgtgc
     1861 cacaggttat ggcggaaaaa ttatcgtgat cagtggaccc tccaatcgcc cgttaatggt
     1921 caacatgcat catcaaaagg tcgatcaagt catgagaaga actccaactg atcctattgg
     1981 cccttttatg gatcaactgg aaagtattat tcgtgtgatg ttccgtgagg aaatatccag
     2041 aaaaccctat ccgcattaag gtgaacggtt tgtcaacatg ggaaggattg ggagtaccgg
     2101 tttaaagcga aaaaggaaat aagggaaata gtagaagagc ccacgggcag ttcgttcata
     2161 aatggtccgg aacaatcata aatcaatgga attaggtgga taggcaatat tcccgtatca
     2221 tattttcaca attgacggga cgagataagg attcagtgct atgaccaggc caatttggaa
     2281 agggcacatc acctttggtc ttgtcacgat tcccgtggtc gttcattcag cggaaaaaca
     2341 atttgacttg catttccggt tgttggatag ccggaatcag gccagagtcc ggtatgaacg
     2401 ggtgaacgag gagacgggtg aagaggttcc atggcaggac attgtgaagg ggtttgaata
     2461 tgaagagggt cggtatgttc tgttaaatga tgccgatttt gaaaaggttg ccattgaagt
     2521 gaataaatca gttgaaatcg aaaattttgt cgacggaaag gatatcggct acgagtattt
     2581 tgataagccc tattatcttg ttcccgataa aaagggtgaa aaagtatatg tgctgttacg
     2641 ggaagtgctt cgtcggagtg gcaaaatcgg gattgccaaa gtcgttatcc gaacacgcca
     2701 gtatttagcc gctcttattc ctcaaggaca tgtcctgatg ctggagcttc tgcgatttca
     2761 tcaggaaatc cgcaatgtga aagacctgga tgtgccggaa gcgagtgtga aatcctacaa
     2821 aatttcggaa aaggaactga ctttggccca acagttagtg gaatccatga gcaccaagtg
     2881 ggaaccccag cgctaccatg atgattaccg tgatgccttg ttgaaatgga tcgaaaagaa
     2941 atcgaaaagt gcccagtcac gaacatccgt tccccatgtc gcaaaagaag agaaatcagg
     3001 ttcaggaaaa gtcgttgata tcatgtcctt attaaagaaa agcgttcaaa aggcccaacc
     3061 ctccggccga gccaaaaaac gcaaatccgc ctcccctcga aaatcccgta aggccagtta
     3121 aaagtaccat tcaccgtaaa ttgcccgctt gactgagcag aagggtcttt gttttagttt
     3181 tttcaggact cagacacacc aggctgctcg catggaatca gacacatggg attaaaagaa
     3241 tatcagggta aacgtcaatt tagtaaaacc ccggaacctc gtgggacgac cttccggaca
     3301 aaccggtctt cttttgttgt ccataagcat gcggcaagcc ggttgcatta cgatttcaga
     3361 cttgagctgg acggcgtgct aaaaagttgg gcggttccga aaggtcccag tttatttcct
     3421 tccctcaaac gtcttgctgt tcaggttgag gatcaccccg tcgaatatgg atctttcgag
     3481 ggaaccattc ccaaagaaga atatggcggt gggacggtca tggtatggga tcggggccaa
     3541 tgggtccctc aaggaaatcc ccagcattcc tacaaccggg gacgattgaa atttcttcta
     3601 aaaggccaaa agctgaaagg gggatggagc ctggtgcgga tgggaggggc aaaggccaaa
     3661 gaagaaaaaa actggttact gatcaaagaa aaggattcgg cagccctcca agggaaacat
     3721 tccgatatta ttgacatggc ccccgcaagt gttgccaccg gacgatcatt ggaaaagatt
     3781 gccgagttaa atcaggaaga aagcaggaaa aacccggctt cccaacggaa gtcgaaaaac
     3841 agttccacaa aaagccattc ctcccgtact ccttcacgga agaataggaa ctccggaact
     3901 tcccgcgtcc ctgatccgtc agaccttcct tccgccgtgt cctcttccca acccgcaacc
     3961 tttcatccac agttggcgac cttggtcaaa acttctccgg aaggggagga ttggctgcat
     4021 gagattaaat tcgatgggta tcgtattgtc agcattgtta aaaacggaag aattcgcctg
     4081 ttgaccagaa acggaaagga ttggaccaaa aaatttcccg acatcgccgg agcgatgaag
     4141 gcattgccgg ttcaacaagg catccttgac ggggaggtgg tggtacttcg atcgaacggc
     4201 accaccgatt ttcaggcctt gcaacatgtc ttgaagggtg aagcctccga ccctctggtc
     4261 tactttgtct tcgatttgcc ctactgcgaa gggtacaacc tgtgtcggac cccgctcatc
     4321 aagcgaaaag aactgctatg ggaattaatc aaatctctgc cggagggggc ggccgttcac
     4381 gtccgttaca gcgatcatat tcagggtcat ggaaaacgtg catttgagca ggcctgtcga
     4441 caggctttgg aaggaattat cagcaaatat gccatgagtc catatcggca agcccgttca
     4501 aggcaatggg ttaaagtcaa gtgtcatcac cggcaggagc tggtgatcgg agggtatacc
     4561 aatccttccg gatccaggaa atttttcgga gccctgctat tgggctatta tgataagcag
     4621 ggacacttac aatatgcggg ccgggtaggc accggcttta cccaccaacg cctttcacgt
     4681 atttatgcac ttctggccaa gcgcgctcag tcccatcctc catttgtccg gctgccttcc
     4741 ggcagggaca ttcgggacgt gcattgggtc catcccgaac ttgtcgccga ggtagaattt
     4801 tttgaatgga cacgtgacgg gatgctgcgc catccttcgt ttgtcggtct tcgtgaggat
     4861 aaaccggcaa aagaaatcat caaagagact ccacaccctg tgaacaatat ttctgaacaa
     4921 ctcaaacctt ccagccggcg atcccatgcc tcgcctgaaa tctctatgga aaggccggaa
     4981 cttgcgctta cgcatcctga aaagattttg tatcctcagc agggcatcac gaaacggaat
     5041 ctggcggtat tttatcaaca gattgccaag tgggtgttgc ctcatgtggc cggtcggccg
     5101 ctgacgctgg tgcggtgtcc tcagggatct caacgaaaat gtttctacca aaaacatgcc
     5161 tcgggatctc ttccggattc cgtgcactgc attctcatcc gagaaaaaga aacgaaaaca
     5221 aagaacacat atctggtaat tgacgacgtg aaagggctga tcgcattggt gcaaatgggc
     5281 gtattggaaa ttcatccgtg gggatgccgg aaggatcgga cggatcgacc tgatcgcttg
     5341 gtcctcgacc tggaccccgg tcccggggtg aaatgggaac agctggttga aggggcccat
     5401 atgttaaaaa gtcggctctc gggagatggt atagaaagtt ttgtgaaaac ctcaggagga
     5461 aagggattac atgtcgtggc ccccctgaca cgccgtgcca cgtgggacca gttaaagcag
     5521 tatacacgga aactggcgat ggaaaccgct caagcgcatc cctcgggatt tgtggccacg
     5581 atgagcaaag ccaaacgaaa agggaaaata tttattgatt atcttcgtaa cagtttcggg
     5641 gccacttcta ttgccaccta tggcaccaga gctctgcccg gagcgcctgt gtcaacccct
     5701 gtgagttggg atgaactctt ttccatctcc ggctcccagg tctttacact ttccactctt
     5761 cctcatcgac tccacactct tagggcagat ccctggaagg gattttttga ccttcggcag
     5821 acactatccc gatcgcgagt ctaattttca aaaacgggtt catgcgcgtg gggggaagag
     5881 gtcctctccg atgccgttca ttttttcttt cgagcacgag tctcagcggc cttcctggca
     5941 gaggcggatt tggcggatgc cgaccgtgag gccgaggctt ttccgccttt ttccccgcct
     6001 tttttagccg gagctgtatt catccctttt cctcgaccgg atccccctgg tttcttccct
     6061 ccaccggtca ttttgttcac agttgcccat gcacgtcgtt ccgcttcttc ttccgatacg
     6121 ccccgctttt catatccttc ttcaattttt tctgcctgac gtttttgttt tccggtatat
     6181 ttggatttgt ctcctctcgg cattttgcac ctcctagaat atgaaaataa tatgacctag
     6241 tacagggatt aaatgagaag tgatatggaa cccaatcatt atggatttat cattgttgct
     6301 gtccaagggt atctgccgag taggcttcgc gttgccccat cggttcgggt ttaccttccg
     6361 tcgtgtctac ccgtgtctgt cgttccattt ctgaattgat ttcggctccg accatgacga
     6421 tgaatgcggt caaccagaac cacatcagca aaatgactgc cgcgccgagt gacccgtacg
     6481 tctggttgta actactgtaa tcattcgcat agatagcaaa agccgacgat cccactatcc
     6541 acatcagtgt ggcgattccg gcaccccatg acacccattg ccatttcggg taattccggc
     6601 agggcgcata ccgatacagg atagccaatc ccgccatgac gatcatggcc aagagcggcc
     6661 aacgactgag agagaggacc gtttgcattt tctggccaaa tcccagggct tccaataccg
     6721 ccggcagcag ggtaatgaga aagagagtca ggatgacaaa aataagacct ccaaccgtta
     6781 atcctaatgc cagcgcggta actttgatga aactccgttc ttcttcttca ttgtaggcaa
     6841 tgttcaagga cgtgatcatt gcagacatgc ctttggacgc gctccaaatc gcaaagagca
     6901 atcccccgat cgctccaaaa cttaaagccg attgcccgct ggtagaaatg gttgtcagct
     6961 gctcttggat caaggtttgg gtgtcttgcg ggaggaagga actgagagaa gtaatctgtt
     7021 gctggacatc agaggcctcg gtaaacagtc catacagcga gacaatggcg gccagcgccg
     7081 gaaagagcgc gaggacggca taaaacgcca cacctgcggc gacaatcgaa acgttatcct
     7141 gtgagatctg atcctttacg cgcaggagga tgtcccacca ccctgtattg gggatttgac
     7201 tgggctgagc cgcattccgg cctctggtgc cggacatctt tttagctgtg tcacgggaca
     7261 tattatatcc tctaattgag aaattttgta cgaataataa atttaattga tgagaaggga
     7321 cccaaactcc agtcagtcag tcacatatca ttactgacca ttgggggaac cggctcatgc
     7381 aactatttat gcggttttcc cctcttcaat tcatgctggc ctgttctttg atgctctcag
     7441 tggccacccg ctgagcctca gcagcggcct ctttggcctt cggcatgatc tccttggcgg
     7501 tggccttagc ttcctccagc aaccggtcgc gcgtttcacc catccattga tcttccttat
     7561 cagaccgagg cagggaaagg ccgatagccg ttccaatggc aaatgttatg gccccgatgg
     7621 tcagggggtt ctcctgcata taccggaaca attcatcctt catgcgtttg cccgcctcgc
     7681 gtgtgcgctc tgtgaattca tctgaccggt gcgacgcctt ttctttccaa tcttccaatg
     7741 attcttgtgc ctggtttggc cattccgaaa tctgttcttt tgcctgttgt cccgaggcct
     7801 gtgctcttgt cttcatggcg tccagggatc ttccccgacg ggatcgttga cggggaatcc
     7861 gcccggcttt aatttcggga gcataatccg gccactcttc atagggatca agggaatagt
     7921 catcgccata tcggtcccta ccaaatctgc ttctggctga tgaggaattg ccggcctgct
     7981 tcatcatcca taggagaccc acgccaataa aggcggcagg taggggattg ttcaggaccc
     8041 gttccaccag gagagattgc cattgattta ccttcacacg ggcattttct tttgccgttt
     8101 cgactttatc tatagctgcg gttttgacct gttccttgat ggtatccgga gacagacggt
     8161 gttgaagtgt atgaaccgtc ccatccaatt ccgctctggt tactcgaatt tgttcgcgaa
     8221 gccgggcagg atcttcaatc tttttcgatt cttgcgcatt taattgagta cgttggtcgt
     8281 ccactgtttg gtctccttta gactgtcaat ggttttttgc ggggcggggt ccatggtttt
     8341 caaatcgctg ataccttttt gaatcaaggc gtagccgatc cccacaacgg ccagtcctac
     8401 aatcagcgcc gaagcccagg cggggatgat taatgccagg gcgaaagtgg ccgcacatag
     8461 gagaaccagc aatcctgcat aggccaacgc tccacctacc gcaataaatc ccacatcctt
     8521 tccggcctgt ttggcttttt gggacatttc aagttttaaa agctgcattt cctgttgaat
     8581 aaggattttt gtttcgttca acaggtcacg gaagagatca ctaagtggtc gttcttcctg
     8641 atataaactc ataacatcct ccttctatgt tctcattaat gtgaataagg attccccgtt
     8701 acatggttgg cgcggcaggg gtcgttgtcg ctgtggattc ttcactccgg aacgattcct
     8761 gccgtccata ggaagaggat tcgtctattc ttcgaactct ggaactcttt aaaaatcttc
     8821 cggccacaag ccccagcata aaggcccccc ctaaaaacgc ttccggatgg cggcgggcaa
     8881 acatttcagt ctcgttcagc aattctccga catcacggga atcgaaatat tgcaccatgc
     8941 gatccatttg ttccgccgcc cgctcggcat agtgggcgac gctttcttta tgctgctccc
     9001 gcaggtgttg actcgtttga cgcacggctt cgacaagccc gctgagttct ccaactccct
     9061 tttgtttctg ggtctccagc gtgctcttga cttttccttg tgcctggttc gccaattcgg
     9121 agccctgtgc gcgaacctgc tctcctacct cactcgcttt ttcctgaacc tgtttcccga
     9181 tctcttttgc tttttccctc atgtcattgg ccattccggt ctcctccatt gaaaagaaaa
     9241 ttcgctcgaa tgataaattc cagccggcat ttgtagttgt gctgactact tatatacaaa
     9301 ggggaggaag tcgtgaactg tcaacaaccc ttaatttccg cgaaatatcg agggcaccca
     9361 tgccagggaa tcccgggatc aggcgtgcgg ctctaagggc ttccggagaa ggatggtgaa
     9421 gactcctgcc aactcagcga taatacggtc gaaggggcaa aggcttcagg ttaattttgt
     9481 ggtatgtatt tcagaaagga gactgacagg ctaattaaga atacccaacc ggctttccct
     9541 tcccatgaga cctgcgggaa cctagaggcg ccgcccaaaa aaatttaggg cggcggaatt
     9601 cattcataac gaaaaatggc tgccaaggac gcacggcctg gcatcttttc ctgggagagg
     9661 gcataaacct tgcctttttt aagtaacgtc tgcaccaccg caaaatctaa gatttccagt
     9721 gattcggcgg actttgtcgg ttctgtttcc acccggtttt gcatgacgtc gatacgcccc
     9781 cattgttgaa tgttccgggc cacgaatagt gtatcgattt ttccctcgtg ggctgctaaa
     9841 accacggttt tcagatcatg ggaagctttg gacgtaccca aatactcttc acacttgatc
     9901 ctggcctctt ctatgccgtg atcggcatcc gctttcacga ccggccagat ctgcccgtgc
     9961 aattcgtgaa ggcttttttc atcagcatta cccgtgattc cttcttcttt gatataaggg
    10021 taggtgttga catctttgta aatcggaatc aaatattcca cgccggt
//
LOCUS       DATIAL010000492        17208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1594781, whole genome shotgun sequence.
ACCESSION   DATIAL010000492 DATIAL010000000
VERSION     DATIAL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 17208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17208
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594781"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..659
                     /locus_tag="VLA60_15120"
     CDS             210..659
                     /locus_tag="VLA60_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10740.1"
                     /translation="MTDGKMNKADWETGVAAIAGVLGAGMGWWWADPVAAGFIAFGII
                     KDGWSQTKDAVTGLVNRAPTSLENEYLNLPDKVNDALLEFPWVGRAQNRLYEHGHLIF
                     GEGFVSLKQNEHISPDQIQDAIQKIKDLDWRLQNFTLTVLPEEKEQQ"
     gene            1233..1409
                     /locus_tag="VLA60_15125"
     CDS             1233..1409
                     /locus_tag="VLA60_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF10741.1"
                     /translation="MDIYQALREDHQEAKQLFSQIEAAEKNSESREKLFLKLKDALEA
                     HSEAEEQVFYTPLQ"
     gene            1351..1668
                     /locus_tag="VLA60_15130"
     CDS             1351..1668
                     /locus_tag="VLA60_15130"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF10742.1"
                     /translation="MPWRRIAKRKSRSSILPFNKKKETKQKIDHANKEHEKVTTLLNE
                     IEAMDHNDENWLKKVTKLKEDVQHHIQEEEGEIFKNAQGILSQQQADHMGEEFKQVKE
                     QKV"
     gene            2201..2866
                     /locus_tag="VLA60_15135"
     CDS             2201..2866
                     /locus_tag="VLA60_15135"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HSF10743.1"
                     /translation="MKHQVEYGLLVWYLGFFVFMGLVPVDPQSWAFANILPLLFVGVL
                     TITHRRWPFSMASYVLLTVFLTLHTIGSHYTYAQVPFGLWLEEFFELPRNHFDRIVHF
                     CFGLLFALPLHELFSRISGMAAWLRSGMVLITLVGLGGLWEILESWVTRIAHPELGLA
                     YLGAQGDIWDPQKDMAATLYGALASLVILVGARKITGTRFAITRERPVELPHAIDHCD
                     KRQ"
     gene            2835..3497
                     /locus_tag="VLA60_15140"
     CDS             2835..3497
                     /locus_tag="VLA60_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HSF10744.1"
                     /translation="MQLTTATSVNNQKSFKDRTLLHGLMAWYVVLWILLAIEPLDRHD
                     WFLENILAIGLVIVLVATYRWFPLSDVSYIFLTVFMTLHAIGAHYTYSKVPLGFWMQD
                     WWGFERNHFDRIAHFSFGLLLAYPLRELFLRRVNVRGFWAYYLPISGILALSGFFEII
                     ESWVALLVSPELGEAYLGTQGDEWDAQKDMTVAVIGAFFTIMLTYALSKLASQKSPPT
                     SP"
     gene            3632..4882
                     /locus_tag="VLA60_15145"
     CDS             3632..4882
                     /locus_tag="VLA60_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10745.1"
                     /translation="MAICLVVISPRFASGEGTDTPLAGEPHHTEIFGQEIYVPPRDRR
                     HVTAINIGMNWIPDGPKRLELLPFGALFVWRNWEDENQRFRGTFSGVFNDIQYQRGLS
                     STSPWFAAMTFENVIIPFGRYEYVEGQAIKDVELEWNYFFAGIGIGYRTPLSPGGQDN
                     AAEFSLTYEPGYRWFDRSSKTSNNFTVPQNTYEGRVHARLRIDKFERNLMELLHHGFA
                     FGGDALYGHRAHWRQWGDPAFETPDNHDDRTFMMASFYAALAGKFPLVPTERHRLIVT
                     MYGGIGKNLDRFSTFRLPGRPTGYEWEALSRPLIPGVAFNELFPRKYAIANFTYRYEA
                     LFFLYPYIRGTWSAIERARFTGDGSIRNQVDMLPAVGGGIVSGAPWGSQIEINYSYNF
                     GIYRDRHGPKLGGNSVVVFWSKLL"
     gene            complement(4966..5169)
                     /locus_tag="VLA60_15150"
     CDS             complement(4966..5169)
                     /locus_tag="VLA60_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10746.1"
                     /translation="MLTIGFILLIIAGLFALIGFGVFTTPILGPARVAFYFLLILSLA
                     TIFYGVVQTEVHVDPQVPTQERN"
     gene            complement(5269..8214)
                     /locus_tag="VLA60_15155"
     CDS             complement(5269..8214)
                     /locus_tag="VLA60_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HSF10747.1"
                     /translation="MLTTSLPILNTAQVKKNRQEDFAYQEIAGELASHLQIFIEGEVR
                     FDEGSRALYATDGSNYRQVPVGVVIPKTVEDVITTVAVCHRHGVPVLSRGGGTSLAGQ
                     CCNVAVVMDMSKYLHTIMELDPASRRARVQPGVVLDVLRNRAEQHHLTFAPDPSTHDH
                     CTLGGMIGNNSCGVHALMGGKTMDNIEELEILTYDGLRMRVGQTTEEDLHRIIRQGGR
                     RGEIYARLQSIRDQYASLIRSRYPDIPRRVSGYNLDQLLPEHHFNIARALVGTEGTCV
                     TVLEATVGLVKSPPWRTLVVLGYPDIYQAGDHLMDILAHQPIGLEGLDDRLVRDMTKN
                     HLHIDKLSLLPEGGGWLLVEFGGDTQQEADDRGRKLMEELRNQAQAPTMKRYDDKVQE
                     QQVWKIRKSGLGATARVPGGDDNWTGWEDSAVAPEDVGNYLRNLRDLMKTYGYQCALY
                     GHFGQGCIHTRINFDLKTRAGIGKYRHFIEEASDLVMQYNGSFSGEHGDGQSRGELLP
                     KMFGPELMNAFREFKRAWDPDWKMNPGKVIDANPLDQNLRLGTSYNPPQLQTHFHYTD
                     DRGSFSRATLRCVGVGDCRSMEGGTMCPSYRATREEQHSTRGRAHLLFEMLQGHPLGK
                     GWRNEAVKEALDLCLACKGCLSDCPVNVDMATYKAEFLSHYYEGRLRPRAAFSMGLIY
                     WWSRLASTMPGVVNAVTHAPILGTLVKKIGGIAPQRDIPRYAMESFTAWFHKRPSPSS
                     AGSKVVLWPDTFNNYFFPHTLKAAVEVLEKIGCEVNIPARPLCCGRPLYDFGMLKTAK
                     LLLRQIINSLGQDIFAGIPVIGLEPSCVAVFRDELLKLFPNDEQAKRLASLTCTFGEF
                     LDTQAGPIAWPQLHERAIVHGHCHQKAVMGMNSEMKILKDIGLDCTLLDSGCCGMAGS
                     FGFKEEHYGISLAIGEQSLLPKIREAAAQHELVVADGFSCREQIVQTTGRNALHTAEV
                     FQRAIKAAE"
     gene            complement(8291..9397)
                     /locus_tag="VLA60_15160"
     CDS             complement(8291..9397)
                     /locus_tag="VLA60_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HSF10748.1"
                     /translation="MMIALPQADTMIARYEVSAYKIPTDMSESDGTYTWDSTTLVVVR
                     IGTRDGEGWGYTYADLATALLIEKTFGSVLKDHDAMDIPECWMTMRRAIRNMGRPGIA
                     SMAIAAVDMALWDLKSRVLNIPLVRLFGRAQAGIPIYGSGGFTSYTDQQLEEQLGTWS
                     EQGISQVKMKVGRHPDQDLHRVHVVRKALQPGTRLFVDANGAYSVRQARWFAERFAEC
                     GVEWFEEPVSSDNLSGLNTIKEHAPASMEIAAGEYGYDPVYFQRMLDAESVDVLQADA
                     SRCGITGLLQVGALCEVRSMPLSAHCAPSLHLHPACALQSMRHIEYFHDHVRIEQMLF
                     DGVPAPINGILYPDLSRAGHGLEFKRADAAQFAL"
     gene            complement(9490..9666)
                     /locus_tag="VLA60_15165"
     CDS             complement(9490..9666)
                     /locus_tag="VLA60_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10749.1"
                     /translation="MNENIEDTNTTLPKRVMGEAIYLAYEFVERKRQDRFYGGYDEAG
                     EKKGVIGNGVDKSK"
     gene            complement(9948..11699)
                     /locus_tag="VLA60_15170"
     CDS             complement(9948..11699)
                     /locus_tag="VLA60_15170"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HSF10750.1"
                     /translation="MTKTAADVLIEKLQNWGVDVIFGLPGDGINGIMEALRQRKDVMK
                     FIQVRHEESAAWMACGYAKYTGKLGVCLATSGPGGLHLLNGLYDAKMEKQPVLAITGH
                     HFHDLIDTHAQQDVNLTRVFEDVVVYNTRIMSATHVENVVDLACRTALSYRGVAHINF
                     PVDLQDSKADRGYSKRNIPHHSMEIFSRGARLPNAADLQQAANVLNEGKRIAIMAGAG
                     ALEATDELEQIAEMLGAPIIKPLLGKASVPDDSPYTTGGIGLLGTAPSQDALEDCDTL
                     LLIGTSFPYMEFYPKPGQARAVQIDLDPARIGLRYPVEVGLVGDCRRTLQELFPMLER
                     NKHRKFLERAQTAMAKWRTLMEERGTRKDKPMKPQVVAWELGKRLSDHAIVSCDSGTI
                     TTWWARQIPAKRGQKHTVSGTLASMACGLPYAIAAQIAYPDRQCIAFVGDGGFSMLMA
                     EFATCVKYQLPVKVFVIKNNTLGQIKWEQMVFLGNPEYGCELHPIDFALVAQACGGTG
                     LTVDDPADCGSIIDQAFQTPGPVLVQAIVDPFEPPMPANITMSQAAKFAESLARGEPN
                     RVKIAMTALSDRVRELV"
     gene            complement(11751..12131)
                     /locus_tag="VLA60_15175"
     CDS             complement(11751..12131)
                     /locus_tag="VLA60_15175"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HSF10751.1"
                     /translation="MSQSMGAKRWAIPEGYIPAKSHGPEPQMTSHETACLLNVSDDPA
                     HVLITIFYTDREPVGPYHVTIPPRRTSHIRFNDLEDPEPIPRDTDFASMFESDVPIVV
                     QHTRLDSRQAENALLSTMAYTDHS"
     gene            complement(12355..13488)
                     /locus_tag="VLA60_15180"
     CDS             complement(12355..13488)
                     /locus_tag="VLA60_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HSF10752.1"
                     /translation="MARSPKRTSGTRKIRYAVVGLGYIAQVAVLPAFAHAKKNSVLAA
                     LISDDPKKLKELGGKYGINGLYSYEQYEECLEKEEIDAVYIALPNHLHCEYTVRAAKA
                     GVHVLCEKPMALSVKECETMIRTADHHDVKLMVAYRLHFEEANLRAIQMVQSGKIGAP
                     RYFQSAFSLPVKDKNNIRLREETGGGTLWDIGIYCLNAARNLFQAEPIQMFAHTAGQG
                     NGRFDEVEEMSAVTLQFPDDRLATFICSFGAADVSEFRIVGTKGQLRVTSAYEYVEPI
                     THYLTVNGKEKKHTFSKRDQFAPQLLKFSDCIVNDTVPEPAGDEGLHDVRIIEALYRS
                     AHKGQPVPLKGVERKRRPTLRQSLRRPPVKKPRLIHAQSPSGS"
     gene            complement(13731..14531)
                     /locus_tag="VLA60_15185"
     CDS             complement(13731..14531)
                     /locus_tag="VLA60_15185"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSF10753.1"
                     /translation="MMNTGRKNSGTLGMALAFALIGGGMSLALSESGQPSKELLLNAN
                     TLIGNTVIDKAGKELGTVKDLLIDQTTGQISYIVLSYGGTFGGTLGIGAENYSIPWAE
                     VKLTKQDDKMLVQVAEAPIGEKRTTKDRAAMRREQASTVQSKDFNPATVETVEGIVEN
                     VDHDMFEPGVTTTDSLIVVDVKTPSGTERVRIAPDNYLKEQGIEIKEGDKVEVTGSRI
                     MRDGESLVIASKVTLKENGKVLAVRKDDGTPKWNKEGGIHSKTSSGKK"
     gene            complement(14810..15073)
                     /locus_tag="VLA60_15190"
     CDS             complement(14810..15073)
                     /locus_tag="VLA60_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10754.1"
                     /translation="MNPLINIKELIGLAVTLSLAGGAFVMLLLGMLLNVFDSPGPVET
                     VTVHELLRDQNPGQISYISFPITEPFQNQIEARRKPIPFSKER"
     gene            15324..15698
                     /locus_tag="VLA60_15195"
     CDS             15324..15698
                     /locus_tag="VLA60_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10755.1"
                     /translation="MNQPKMDMWPVVLGIIVILLMNASHLPQGMSGSGVPKPSPSPQA
                     IEQNSPGLNKEPRNTQPIVPPNDSEMVVEPDIPPNPEAVVTPPVVDPEMVVDPTTREP
                     MTEEKLERLTPEALEKNAPEEK"
     gene            complement(15748..16692)
                     /locus_tag="VLA60_15200"
     CDS             complement(15748..16692)
                     /locus_tag="VLA60_15200"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HSF10756.1"
                     /translation="MSARILSLGSVNVDIQLRADRWPEAGETLLGQDFLMAGGGKAAN
                     VAWLARYLGIEAQLIARIGSDSLQEIALHPLRTLGVDLSTIRSIKNQATGVSVIMVQP
                     NGRKGIIMAPNANAAWSEEDEELVKYAIQTAPPGSVLSVNLEVPLPIVKQAVRQAEEC
                     GMPIVLDPSPSERLDEELLKSGCYLTPNQSEAQRLTKIPIENHDHALEAATRIRQHGN
                     KGVCVKLGTGGCAIATRDVQFAISAPEVHAVDTTGAGDAFAGAFSVAMLEHQPPEQAA
                     AYAVAASTHAVTGYGSQSSYPTRRQIESLLQRVVMKKI"
     gene            complement(16689..>17208)
                     /locus_tag="VLA60_15205"
     CDS             complement(16689..>17208)
                     /locus_tag="VLA60_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HSF10757.1"
                     /translation="LTVSELESILANLGYPFSLKLLEKNIAYYQQRTTHGSTLSRVVF
                     GWIQARSDRSGSWKFLAEALGQDVHEIKRGSTGEGIHLGAMAGTIDLMQRCYGGIDCE
                     NNVLTVNPCLPETLERLAMKVQFRQHHINVQISPKSLCLDVSAGPNFPLVINVRGTRH
                     VFGTPTQQDFPL"
BASE COUNT         4161 a         4296 c         4260 g         4491 t
ORIGIN      
        1 aaaatcctct gcgatcggga gccagggttc tcgtgaagag aatccggcgt ggagatccgg
       61 gttccattgc atcggtgtgc gttccggatc gcggtttcgc ccggtcctcc ggccctgagg
      121 atcgatagcc tgttcggcag gaatcgggac tccgcgcatg ccgacgcgtc aagttgaaat
      181 atgcccagac gttgaacgat aaaattctct tgactgacgg aaaaatgaat aaggccgatt
      241 gggagaccgg cgtcgcggct attgccggcg tgttgggagc cggaatggga tggtggtggg
      301 cggatcctgt ggccgccgga tttattgcat tcggcatcat aaaggatggc tggagccaga
      361 caaaggacgc cgtgacggga ttggtcaatc gcgcccctac ttccctcgag aatgaatacc
      421 tgaacctccc cgacaaggtg aatgatgcac ttctcgagtt cccctgggtt ggccgcgcgc
      481 aaaaccggtt atatgaacat ggtcatctca tttttggaga agggtttgtt tccttgaagc
      541 aaaatgaaca tatctctcct gatcagatac aggatgccat tcaaaagata aaagatcttg
      601 attggcggct gcaaaacttt accttgacgg tattgcctga ggagaaggaa caacaatagt
      661 tcgttcttaa ggggggccgg attgttgtgg gggcagccaa aattgtacag atttgggcac
      721 atataaacgt atggtgcctt gctgggtgaa aaccggacgt gagtccatcg agcttatcat
      781 tatcaaatga taatcacgtg aaaagtttag tgcggaagct gcggtgggat acccccgggc
      841 tgacagttcc ggaagaggga ttgtccactt cctataccaa gtacacctgt caataaaaat
      901 ccagacagca tggtccattc gttggatccc aagacgattg cgacgctaag aaatgccctg
      961 gcgccgggaa cctgaagcaa taatcctaca tcgtgaccaa tggctccgat tgaatgtgag
     1021 tttgccctga taagtattcc cctctcgaaa gcttcattca tgcagcggtg atgtgactat
     1081 gatcacaccg tgtcagacgg atgtaggtgt acatttttgc aagcccctcc agcaattcgt
     1141 ccatacctca cataatccgc cccgtggtgt actacggaac atcatatgta tggttcagga
     1201 gaaaaacttt tcattcataa gtggagggta taatggatat ttatcaggcg ctcagagaag
     1261 atcatcaaga agctaaacaa ctattcagcc aaatcgaagc cgcggagaag aacagtgagt
     1321 cacgggaaaa actgtttctc aaacttaagg atgccttgga ggcgcatagc gaagcggaag
     1381 agcaggtctt ctatactccc cttcaataaa aagaaggaga cgaaacaaaa aatcgaccat
     1441 gccaacaaag agcacgaaaa agtcaccacg ttgttgaatg aaatcgaggc aatggatcac
     1501 aatgatgaga attggttgaa gaaggtcacc aaactgaaag aagatgtgca acatcatatc
     1561 caggaggaag aaggcgaaat ttttaagaac gctcagggta ttctttcaca gcaacaggcc
     1621 gaccatatgg gtgaagaatt caagcaggtc aaggaacaga aggtctagga aaagaggact
     1681 tggacttgcc tcggagacgg acatctatga ccgggatgga gcaagacaat ggcggataaa
     1741 tggggattgt gtaaaaggtg ccaatggtgg cagatcgaac catctgcaaa tatcgaagat
     1801 gctacaatgg gactgtgtat cgatgaaaac ctacaaccgt atcgattgtt ggtctccggc
     1861 aatagtggat gcaatcgatt catgaaaggt aatccggcac aagcggaagg gtcctcttcc
     1921 cggcctccga ccgccgagcc cacccgatag tggaacgggg tttttgactg ccggtgaaat
     1981 gggggaattt gaaggctgtc tctcattcaa gaacgtcgga agggatccat caaaggaagg
     2041 aatggcgaac agggtttgtc acgcccggta gtcatccatg ggttcaagtc tcagtccaag
     2101 aacggaaatt atcccgttga cttgtcattg aagaatccaa cggacccata atattggcag
     2161 gaggaatgaa tcgttccgaa cgccctgagg gagagtctat ttgaagcatc aagtggagta
     2221 cggattattg gtctggtatt tggggttttt cgtgtttatg ggattggttc ccgttgaccc
     2281 ccaaagttgg gcctttgcta atatcctccc gcttctcttc gtcggtgtgt taaccattac
     2341 tcatcggcgg tggccatttt caatggcttc ctatgttctc ctcaccgtgt ttctgacgtt
     2401 acatacgata ggttcccatt atacttacgc acaagtgccg ttcggcctct ggctggaaga
     2461 gttctttgaa ttgcccagga accatttcga tcggattgtg catttttgtt tcggtttgct
     2521 cttcgccctg ccattgcatg aactcttctc ccggatttcc ggaatggctg catggctccg
     2581 ctccggcatg gtcctgatca ccctggtggg gctggggggg ttgtgggaaa ttctcgaatc
     2641 ctgggttacc agaatcgccc atcccgaatt aggtctagcc tatctgggtg cccagggaga
     2701 tatctgggat ccacagaagg atatggccgc gacattatat ggagcattag cctctctggt
     2761 gatcctggta ggagcacgga aaatcacggg gacccgtttc gcaattacgc gggaaaggcc
     2821 ggtggaactt ccgcatgcaa ttgaccactg cgacaagcgt caataatcag aagtcgttta
     2881 aggaccggac tctccttcac gggttaatgg cctggtatgt ggtgctttgg atattgctcg
     2941 ccatagaacc gctcgaccgg catgattggt ttctcgaaaa tattttagcg ataggtctgg
     3001 tcattgtcct agtcgcgacg tatcgatggt ttcccttatc ggacgtttcc tatattttcc
     3061 tcacggtgtt tatgaccctc catgcgatcg gagcccatta tacctattca aaagtcccgc
     3121 tgggattctg gatgcaggat tggtggggat ttgagcgcaa tcattttgac cggatcgcac
     3181 atttttcatt cgggttgctc ctggcttatc ctttaaggga gttattcctt cgccgggtca
     3241 atgtgcgtgg gttctgggct tattatctcc ccattagcgg aatattggcc ctcagtggat
     3301 tcttcgaaat tatcgaatcg tgggtggctc ttctcgttag tccggaattg ggggaggcct
     3361 atctgggcac tcagggggac gaatgggatg cacaaaaaga tatgacggtg gcggtcatcg
     3421 gggccttttt cacaatcatg ctgacctatg ctctttccaa gttagcgtca caaaaatctc
     3481 caccgacttc tccctagcat tttttcaatt tccaatgctt cgactctcca ccgcgcattt
     3541 tatcccgatc cacgctcaaa gggatgattt caggcgacca atctggcacg agtctttccg
     3601 cccagttctt actcatctag gcatgctggt cttggccata tgtctggtgg tgatttcacc
     3661 tcggtttgct tcgggagaag gaaccgacac gccgcttgcc ggagaacccc atcatacgga
     3721 aatctttgga caggaaattt atgtccctcc acgggatcgc cgacatgtta cggcgatcaa
     3781 tatcgggatg aattggattc cggacggacc caagcggtta gaacttctcc ctttcggtgc
     3841 gttgtttgtc tggcgcaatt gggaagacga aaaccagcgg tttcggggaa cattctcagg
     3901 agtattcaat gacatccagt atcagcgggg tctgtcatca accagcccat ggtttgccgc
     3961 catgacattt gaaaatgtga tcattccatt cggccgatac gaatatgtcg aaggacaggc
     4021 gatcaaagac gtggaactgg aatggaatta tttctttgcg ggaattggta tcgggtatcg
     4081 aacgccgtta tccccggggg ggcaggataa tgccgctgaa ttttctttaa cgtatgaacc
     4141 cggttaccgt tggttcgatc gcagttcaaa aacgtccaat aatttcactg tgccccagaa
     4201 cacgtatgaa ggccgtgttc atgcccggct tcggatagat aaatttgaac gaaatcttat
     4261 ggagttgctt catcacggat tcgctttcgg aggtgatgcc ctctatggac atagggccca
     4321 ttggcgtcag tggggagatc cggcattcga gaccccggat aaccatgatg accgcacatt
     4381 tatgatggcc agcttctatg ctgccctggc gggaaagttc cctttggtac cgacagagcg
     4441 gcacagattg attgtcacga tgtatggtgg aatcgggaag aaccttgacc ggttttccac
     4501 tttccggttg cctgggcgtc ccacggggta tgaatgggaa gcgctctccc gtcctctcat
     4561 tccgggagtg gccttcaatg aattgtttcc tcggaaatac gcgattgcaa atttcaccta
     4621 ccgatatgag gcgttgtttt ttttgtatcc gtatattcgt ggaacctggt ccgcgatcga
     4681 acgagcccgt ttcaccggag atggttccat tcgtaatcag gtcgatatgc tgccggcggt
     4741 gggaggcgga atcgtcagtg gggctccttg gggttcacaa atagagatca attatagtta
     4801 taactttggc atataccgtg atcgccatgg gccgaagctg ggagggaata gtgtcgtggt
     4861 gttctggtca aagcttcttt aacacgaagc gaaattctct cgcatccgct ttttttcatg
     4921 aggatcagga tgaggatctt ttccttctct tttttttcga tacgattagt tccgttcttg
     4981 agtcgggacc tgtggatcga catgcacttc tgtttgaacc actccataga agatcgtcgc
     5041 cagggacaga atcaacaaaa aataaaaggc cacacgcgcc ggtcctaaaa taggggtcgt
     5101 aaagactcca aatccaatta acgcaaaaag tcccgcaatg atgagtaata tgaaaccgat
     5161 ggtgagcatg ggtgcctcct ttttcagtag gaaaagagcc gatatttttc aaacataatt
     5221 atttcggcca cgggttcctt tgcccggcca ttcggaagag ccggatgctt attcagcagc
     5281 ttttatggct ctttgaaaga cttcagccgt atggagagcg ttccgcccgg tggtttgcac
     5341 gatctgttcg cgacaactga atccatcggc aacgactaat tcatgttggg ccgccgcctc
     5401 tcgaatcttc gggagaaggc tctgttctcc aatggcaagg gaaatcccgt aatgctcctc
     5461 tttaaatccg aatgaccccg ccatgccgca acatccggaa tcgagaagag tgcaatctaa
     5521 tccaatgtcc tttaaaattt tcatttcact attcatcccc atgacggcct tttgatgaca
     5581 atgaccatgc acaatggctc gttcatggag ctgaggccag gcaatgggtc ctgcctgtgt
     5641 atcaagaaac tctccaaaag tacatgttaa actggctaac cgcttcgcct gttcatcatt
     5701 gggaaacaat ttaagaagtt cgtcgcgaaa gaccgccacg caactgggtt caaggccaat
     5761 gacggggatg ccggcgaaga tatcctgacc cagggaattt atgatttgac gcagcagaag
     5821 tttggcagtt tttaacatgc cgaaatcata taagggacgt ccgcaacaga gaggccgtgc
     5881 ggggatattt acctcacaac caattttctc cagcacttcg actgccgctt tcagtgtgtg
     5941 gggaaaaaaa taattattaa aggtgtcagg ccataacacc acttttgacc ctgccgaaga
     6001 aggagatggc cgtttatgaa accaggcggt aaaggattcc atggcatagc gaggaatgtc
     6061 tcgttgagga gcaatacccc caattttttt gaccagagtc cctaaaatcg gggcatgagt
     6121 cacggcattc accaccccag gcatggtgga ggcgagcctt gaccaccagt aaatgagtcc
     6181 catggaaaag gctgcgcgtg ggcgaaggcg gccttcataa tagtgagaca aaaattccgc
     6241 cttataggtg gccatatcca cattcaccgg acagtcgctt aggcagcctt tgcaggccag
     6301 gcacagatcc aatgcctctt tgaccgcttc gtttcgccag cccttgccta agggatggcc
     6361 ttgtaacatt tcgaacagca ggtgtgcccg tccgcgagtg gaatgttgtt cctcccgtgt
     6421 tgctcgataa ctggggcaca tcgttccgcc ttccatgctg cggcaatctc ctacgccgac
     6481 acagcgcaac gtggcccgcg aaaagctccc gcgatcatcc gtataatgga aatgggtttg
     6541 cagttgtgga ggattatagg aggttcccaa ccgcaagttc tgatcaaggg gattggcatc
     6601 aatgactttt cccggattca ttttccaatc gggatcccag gctcgtttaa attcccgaaa
     6661 cgcattcatt agttccgggc cgaacatctt tgggagcaat tcaccccggg actgcccatc
     6721 tccgtgttca ccggaaaagg aaccgttata ctgcatcacg aggtcggagg cttcttcgat
     6781 gaaatgacgg tatttgccaa ttcccgcgcg cgtttttaaa tcgaaattta tgcgagtatg
     6841 gatgcatccc tggccaaaat gaccatacag cgcacactga tacccatacg tcttcatcag
     6901 gtcgcgaaga tttcggagat aatttcccac gtcttccggt gcgacggccg aatcctccca
     6961 tccggtccaa ttatcatccc cgcccggcac tcgagccgtg gccccgagac ctgatttacg
     7021 aatcttccat acctgttgtt cttgaacttt atcatcatag cgtttcatgg taggggcttg
     7081 agcttggttg cggagttcct ccatgagctt ccgcccccgg tcatctgctt cttgttgggt
     7141 gtctccgccg aattcgacca ggagccatcc cccgccttcc ggaagcaggg atagtttatc
     7201 aatatgcaaa tggtttttgg tcatatcacg cacgagccta tcgtccagtc cttccaatcc
     7261 gatcggctga tgcgccagga tgtccatcaa atgatctccg gcttgataga tgtccgggta
     7321 tcccagaaca acgagtgtcc gccatggcgg acttttgact aacccgaccg tcgcctccaa
     7381 cacggtcaca caggtgccct cggtccctac cagggctcga gcgatattaa aatgatgttc
     7441 ggggagtaat tgatcaagat tgtatcccga cactctcctg ggaatgtcgg gataacggga
     7501 tcggatcaac gaggcatatt gatctcgaat ggattgtagc ctggcataga tctcaccgcg
     7561 tcggcctcct tgccggataa tgcggtgtag gtcctcttca gtagtttggc ccacgcgcat
     7621 ccgcaatcca tcataggtga gaatttccaa ttcctcgatg ttatccattg tttttccacc
     7681 catcagggca tgcactccgc aggaattgtt gccgatcatc ccacccagcg tacagtgatc
     7741 atgtgtggac ggatcagggg caaacgtcaa atgatgctgt tccgctctgt tgcgcagcac
     7801 gtcgagcacg acccccggtt gcacgcgcgc acgcctggag gcgggatcca attccatgat
     7861 ggtatggagg tatttcgaca tatccataac gacggccaca ttacaacatt ggccggccag
     7921 actggttcct ccgccgcgtg agagaactgg gactccatgc cggtgacaca ctgccaccgt
     7981 ggttatcaca tcttccacgg ttttgggaat gacgaccccg acgggcactt gacggtaatt
     8041 cgatccgtca gtcgcatata acgcccggct tccttcatcg aaacgcactt ctccttcaat
     8101 gaagatctga agatgagaag ccaactctcc tgcaatctcc tgataggcga aatcctcctg
     8161 ccggttcttt ttgacctgag cggtattcag aatgggcagt gatgtggtta acatgaagat
     8221 tttatccttg tgcggtgcac cttacgaaat tccctatggg atccttctcg gcaggaaatc
     8281 cccgcacggt tcagagagca aattgagcgg catccgcccg tttaaattcc aagccatgtc
     8341 cggcacgact caaatcagga tagagtatgc cgttaatcgg agctgggacg ccgtcaaaaa
     8401 gcatttgttc tatccggaca tgatcgtgaa aatattcgat gtgtcgcata gactggagcg
     8461 cacaggccgg atggaggtga agtgacggcg cacaatgagc tgacagcggc atagaccgga
     8521 cttcacataa cgccccgact tgtaaaagtc cggtgatacc gcaacgcgat gcatcggcct
     8581 gtagcacatc cacactttct gcatccagca ttctttggaa atagacggga tcatacccgt
     8641 attctcctgc ggcgatttcc atggatgccg gcgcatgttc ttttatggtg ttgaggccgg
     8701 agaggttatc ggaagacacc ggttcctcaa accattccac cccgcattcg gcaaatcgtt
     8761 cggcaaacca tcgggcctgt ctcactgaat aggctccatt ggcatcgaca aagaggcggg
     8821 tgccgggctg aagggctttg cggaccacat ggaccctgtg cagatcctgg tcaggatgac
     8881 gtccaacttt cattttgacc tgcgagattc cctgctcgct ccatgtcccc aattgttcct
     8941 ccagctgttg gtcagtatag gaggtgaatc ctccacttcc gtagattgga attccagcct
     9001 gagcacgccc gaataacctg accagtggaa tattcaatac ccgcgatttt agatcccata
     9061 gagccatatc cacggcagcg atggccatgg aagcaatgcc gggtctcccc atattccgta
     9121 tggcccgtct catggtcatc caacattcgg gaatatccat ggcgtcatga tctttcaaca
     9181 cggaaccgaa ggtcttttca atcaataggg ctgtggcgag gtcggcatag gtatatcccc
     9241 atccttctcc atcccgcgtt cctatccgca ccaccaccag cgttgtcgaa tcccaggtat
     9301 acgtcccatc cgattcagac atgtcagtgg gaattttata ggccgagacc tcatagcggg
     9361 cgatcatggt gtcagcctgc ggcagagcga tcatcatttc actagggcga caacttgtcc
     9421 ggcagatgta gcggatccag aggaagaaca acctgaatca tacaatccaa tcttccacct
     9481 tggatgccct cacttacttt tatcaacacc gttgccaatc acacccttct tttcaccggc
     9541 ctcatcgtat ccaccataaa accggtcttg tcttttcctt tcaacgaatt catacgcaag
     9601 ataaatggcc tcccccatca cacgcttagg gagagtggtg ttggtgtctt cgatgttttc
     9661 attcatccat tccacctctg tatttatcga aataggggaa acaacccatt tacaacaaat
     9721 ttgaattctt gaaaaagaac cctttaaaaa tttatcacca caattaccat tccttcaccc
     9781 attcctcata aacactgccc ttttttcaat aatcgtgcaa ttggacagaa cccttgacga
     9841 gaaaaaatgg cgcagagaag aacggagaga agaaattttt aaccttctta tttctgaagc
     9901 gtcgctaggg tccccttttc gaggctacaa atgtctccat tattctgtta cacgagttcc
     9961 cgtacccgat ctgacaaggc cgtcatggca attttcaccc ggttcggttc gcctcttgcc
    10021 aacgattctg caaatttggc ggcttggctc atcgtaatgt tggcgggcat cggaggttca
    10081 aaaggatcga cgatggcctg taccaatacc ggtcccggcg tttggaaagc ctggtcgatg
    10141 atggaaccac aatccgcagg atcgtcgacg gtcagtccgg ttcctccgca tgcctgagcg
    10201 acgagggcaa agtcgatggg atgcagttcg cacccatatt cgggatttcc cagaaacacc
    10261 atctgttccc atttaatctg gccaagcgta ttatttttga tgacgaacac tttcacgggc
    10321 aattgatatt tgacacaggt ggcgaactca gccatgagca tcgaaaaacc cccatctccc
    10381 acaaaggcga tacattggcg atccggatag gcgatttggg cggcaattgc atagggtaag
    10441 ccgcaggcca tggaggccag agtgccggat accgtgtgct tctgccctcg tttggccggg
    10501 atttgcctgg cccaccaggt cgtgatcgtt cccgaatcac aggaaacgat ggcgtggtca
    10561 gaaagacgtt tgccgagttc ccaagccacc acctggggtt tcatgggttt gtcttttcgg
    10621 gttccgcgtt cttccatcag tgtccgccat ttggccattg ccgtttgtgc ccgttccaaa
    10681 aatttccggt gtttattccg ttccaacatc gggaagagct cttggagggt tctccggcag
    10741 tcgccaacca ggccgacttc caccgggtac cgcagtccga ttcgcgccgg atcaagatcg
    10801 atctgcacgg ctcgggcctg gccgggtttg ggataaaatt ccatataagg aaatgaggtt
    10861 ccgatgagaa gcagggtatc gcaatcctca agggcgtcct gagagggcgc agttcccaat
    10921 aatccgatgc cccccgttgt atagggactg tcgtccggca ccgacgcttt tcccaataac
    10981 ggcttaataa tgggcgctcc cagcatttct gcaatttgtt ccagttcatc ggtggcttcc
    11041 aaagccccgg cccctgccat gatggcaata cgttttccct cattcaggac gttggcggcc
    11101 tgttgtaaat ctgctgcatt gggtaatcgc gcgccacggg aaaaaatttc catcgaatgg
    11161 tgaggaatat tgcgtttgga atagcctctg tctgcttttg aatcttgaag atcgacggga
    11221 aaatttatgt gagccacccc gcgatagctc agagcggtcc gacaggctaa gtcgacaaca
    11281 ttctccacat gcgtcgcact catgattcga gtgttgtaga ccacgacatc ttcaaagact
    11341 ctggtaagat tcacatcctg ttgggcatgg gtatcgatca aatcgtgaaa atgatgaccg
    11401 gtaatagcca gtaccggttg tttctccatt ttggcgtcat acaaaccatt cagcagatga
    11461 agccctccag gccctgaggt ggccaggcac acaccaagct ttcccgtgta tttagcatac
    11521 ccgcatgcca tccaggccgc tgattcttcg tggcgtacct gtatgaattt cataacatct
    11581 ttgcgttgac gcagggcttc cataattcca ttaatgccgt ctccgggaag accgaatatg
    11641 acgtccacgc cccaattttg aagcttctct atcaacacgt ctgcggcggt ttttgtcatg
    11701 ctctgagttc ctctcttgtt ttctcaaggt ggaaataacg atttccgtct ttacgaatga
    11761 tccgtatatg ccatggtaga caggagagca ttctcggcct gtcgggaatc cagcctggta
    11821 tgctgcacca caattggcac atcggattcg aacatgcttg cgaaatctgt atccctgggt
    11881 ataggttcag ggtcttccag gtcattaaaa cggatatgac tcgttcgacg aggagggatg
    11941 gtgacatgat aaggccccac cggctcccgg tctgtataaa aaattgtaat aagcacatgc
    12001 gccggatcgt cggaaacatt gaggaggcat gcggtttcat gactcgtcat ctgcggctcc
    12061 ggtccatggc ttttggcagg gatatatcct tcaggaatag cccatcgttt agcccccatt
    12121 gattgactca tgacattatg tcttcttctg cggaagttct ttgggctttt ttaacatgtc
    12181 cttgccgatg gcgacgttcc cattccctat ccaatgccgc ctcaacaaga taggaaggta
    12241 aagacggatc gatattttcc gaggaatgtg acccttgaga accaagggtg ttcgactgtt
    12301 cattcattga ataaattcct ttcttttttt tataggatga atgaaaatgg ttcatcatga
    12361 tcctgacgga ctctgggcat ggatgagtcg gggtttttta accggcggac ggcgaaggct
    12421 ttgccgaagc gttggacgcc gttttcgctc cacgcctttg agcgggaccg gttggccttt
    12481 gtgggctgaa cgatacagag cttcgataat gcggacatca tggagaccct catcgcctgc
    12541 cggttcagga accgtatcat tcacaatgca atccgaaaat ttcaaaagtt gtggagcaaa
    12601 ttgatcacgt ttggaaaagg tgtgcttttt ctctttcccg ttcaccgtaa ggtaatgagt
    12661 aatgggttcg acatattcgt aggccgatgt cacccgcagt tgccccttgg tccccacaat
    12721 ccgaaattcg gaaacgtcag cggctccaaa actacagata aaggtggcca accgatcatc
    12781 gggaaattga agcgtcaccg cgctcatttc ttccacttcg tcgaatctgc cgttaccctg
    12841 tccggcggtg tgggcaaaca tctgaatagg ttccgcctga aatagattcc gggcggcatt
    12901 caaacaataa ataccgatat cccacagcgt tcctcctccg gtctcttccc gtaaccggat
    12961 attattttta tctttgaccg ggaggctaaa tgccgattga aagtaccggg gtgcgccgat
    13021 tttccctgat tgaaccattt gtatggcccg aaggttggcc tcttcaaaat gaagccgata
    13081 ggcgaccatt aatttgacat catgatggtc cgctgtgcga atcatggttt cacattcctt
    13141 gactgataag gccatgggtt tttcgcacaa tacatgaacg cccgctttgg cagctcggac
    13201 ggtatattcg cagtgcaaat gattgggaag cgcgatatac accgcatcaa tttcttcctt
    13261 ttccagacat tcttcatatt gctcatagga atagagaccg ttgatgccgt attttcctcc
    13321 caattccttc aattttttgg gatcatcaga gattaaagct gccaataccg agtttttctt
    13381 tgcatgtgca aatgccggaa gtacggcgac ttgagcgata tagcccagcc ccactaccgc
    13441 gtaacgaatt ttcctggtgc cagatgttct tttgggcgaa cgtgccatcc tgggatctcc
    13501 ttgaaatcgt gtcaaaagag taaatcagag cagttccttc acagctttta acaatgtttt
    13561 tatcgaaaat gaactggaaa gaaccctggt tccaagaaat atcagccagg actgacaata
    13621 caggcctgaa gagattccaa agagagggag aaggtttggg agggggagaa caaagtcaaa
    13681 cgggaaaaag ccccgatggc tttttcccgt tgacagagaa tagagtaatt ttatttcttt
    13741 ccggaagatg tcttactatg gattccaccc tctttgttcc atttgggagt gccgtcatct
    13801 ttccggaccg ccaaaacttt tccattttct ttcagggtaa cttttgaggc gatgaccaga
    13861 ctttccccat cccgcatgat ccttgacccg gtcacttcca ctttatcgcc ttccttgatt
    13921 tcgattccct gctccttgag atagttatca ggggcgatgc gcacccgttc tgttcccgat
    13981 ggcgttttga catccaccac tatcaaacta tcggtagtgg tcactccggg ctcaaacatg
    14041 tcgtgatcca cattttccac gatcccttct accgtttcga ctgttgcagg attgaaatcc
    14101 ttactctgga cggtagaagc ttgctcgcgg cgcatagctg cgcggtcttt tgttgttcgt
    14161 ttttcgccaa taggggcctc ggctacctga accaacattt tatcatcctg tttggtcagc
    14221 ttcacctccg cccagggaat cgaataattt tccgccccga tccctagagt gccgccgaat
    14281 gtgcctccat atgagagaac gatgtacgaa atttgccctg tggtttgatc aattagtaaa
    14341 tctttgactg tcccgagttc tttgcccgcc ttgtcaataa ccgtattccc gatgagggtg
    14401 ttggcattaa gaagaagttc cttggatggt tggcctgact cgctcaaggc taaggacata
    14461 cctcctccta tgagagcaaa ggctaatgcc attccgagag tgccactatt tttgcgtccg
    14521 gtgttcatca tgataatttt ccttttgtaa gagtttggta cacggtagtg attagaattt
    14581 gtttttttga ttcacgagta gattgtgtgg ccacgtgccg aagaaacgtg cttggttttc
    14641 tcctttttga gttagaacta tttctatgtt tggtttaaat taccctgcgt ttccaggtgg
    14701 ttaaagaata ttcttttctc ccacggccat cttcaacctc cgcttcctat tggatatgcg
    14761 ccacgcgatt cacgaggctg gtagcctttt ccccgcagct attcttagct tatctttctt
    14821 ttgaaaaagg tataggtttt cgacgagctt caatttggtt ttgaaagggt tcagtgattg
    14881 gaaatgatat ataggaaatt tgtccggggt tctgatccct caacaattca tgaaccgtta
    14941 ccgtttccac tggacctggc gagtcaaaaa catttaaaag catgccgaga agaagcatca
    15001 cgaacgcacc tcccgccagg ctaagggtta cagccagtcc gattaattct ttgatgttga
    15061 tgagtgggtt catgagagtt ttcctaggag tgaaattatc tatatgttca taagttccct
    15121 tttttctttt caaattgtaa agaaacgaaa atgaaatggg actgaataaa acccttgcat
    15181 tggccgctat cttcccttaa aaatctgacc ggacttttaa taaatccttt ttcccagggt
    15241 tcttgttaga tgcttttggt tttttttcgg aagataatgt atggagagag ataaggaatc
    15301 aaattaaaaa agaggtgaaa aacatgaacc aaccaaaaat ggatatgtgg ccggtggtgc
    15361 tgggaatcat tgtgattctt ctgatgaatg cctctcatct cccccagggg atgtcaggat
    15421 caggtgtccc aaagccgtca ccttcacctc aagcaatcga gcaaaattca ccggggttga
    15481 ataaggaacc tcggaacaca cagccgatcg ttccaccgaa tgattcggag atggtggtgg
    15541 agccggatat tcccccaaat ccggaggcgg tggtgactcc accggtggtg gacccggaga
    15601 tggtggtgga tccgaccacc cgagagccca tgaccgaaga aaaactggaa cggctgaccc
    15661 cggaggcatt ggagaaaaat gctcccgagg agaaataacg acagattcca tgagcagatt
    15721 tttaaataat tatccgttga atctgtttta tatttttttc atgaccaccc tctgcaagag
    15781 agattcaatt tggcgtcggg tggggtagga agattgggag ccatagccgg tgacggcgtg
    15841 ggttgaggcg gcaaccgcat atgcggcggc ctgctctggg ggttgatgtt ccagcatcgc
    15901 cacactaaat gctcccgcaa acgcatcacc cgccccggtg gtgtccacag catggacctc
    15961 cggagcggaa atggcaaact ggacatccct cgtggcaatc gcgcatcccc ctgttcccaa
    16021 tttgacacaa actcccttat ttccgtgttg tctaattctg gtagccgctt ccaaagcgtg
    16081 gtcatgattt tcaattggga tttttgtcaa tcgctgagcc tctgattggt tgggagtcaa
    16141 ataacagccg gattttaata attcctcatc caatctctca gacggtgaag gatccaggac
    16201 aatgggcatt ccacattcct cagcctgtcg aactgcctgc ttgacaatgg gcagggggac
    16261 ttccaaattc acggacaata cggatccggg tggtgccgtt tgtatggcgt atttgactaa
    16321 ttcttcatcc tcttcggacc aggcggcatt ggcattcggt gccatgataa tccctttccg
    16381 tccgtttggt tgaaccatga tgactgacac tcctgtggct tggtttttta tgctgcggat
    16441 agtggataaa tcgactccaa gtgtccgtaa ggggtgaagg gcgatttcct gaagtgaatc
    16501 agatccaatt cgagcaatta attgcgcctc gatgcccaga taacgggcta accaggccac
    16561 attggcggcc tttcctccac cggccataag aaaatcctga cccaacagtg tttcccccgc
    16621 ttccggccaa cgatccgcac ggagttgaat atctacattc acgcttccaa gagataagat
    16681 tcgagcgctc ataagggaaa atcctgctgt gtcggtgttc caaaaacatg gcgtgttcca
    16741 cgaacgttga tgacgagagg aaagttcggt cctgcagaga cgtcaaggca caagctcttt
    16801 ggggaaattt gcacattgat gtgatgctgt cgaaactgaa ccttcatcgc taacctctca
    16861 agggtttccg gaagacaagg gttcaccgtt aacacattat tttcacaatc aattccacca
    16921 tagcaacgtt gcataagatc gatggttcct gccatggctc ctaaatggat gccttcgccc
    16981 gtcgatcctc tcttgatttc atgaacatcc tgtcccaggg cctcagccag aaacttccat
    17041 gagccgcttc gatcggaacg cgcctgaatc catccgaaca caacgcgact gagcgtcgaa
    17101 ccatgagtgg tgcgttgttg ataataggca atattctttt ccagcagctt tagagaaaac
    17161 ggatatccta aattcgcaag gattgattca agttcagata cagtcagg
//
LOCUS       DATIAL010000493         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1379417, whole genome shotgun sequence.
ACCESSION   DATIAL010000493 DATIAL010000000
VERSION     DATIAL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379417"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2119)
                     /locus_tag="VLA60_15210"
     CDS             complement(<1..2119)
                     /locus_tag="VLA60_15210"
                     /inference="COORDINATES: protein motif:HMM:NF018620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HSF10758.1"
                     /translation="MNSRPYARMVSTLLLMVIGGGCTLLVGLQLDQQFGKPSPQNRLA
                     TTPGVQVRTVTYVEDVKPILENRCVVCHGCYDAPCQLDLSAPEGIERGANKDLVYHAG
                     RLLAMEPTRLFVDAHSPAEWREKRFYPVLNERVQTKEANLNGGLMAKMLELKRQHPLP
                     TVSPLPASFDFSLERAQYCPMLEEFDDFAKTHPLWGMPYGLPDLSDGEYRTLRTWLEE
                     GAGYATPPPLGASPLANIPKWETFFNGNSLKEQLMSRYLYEHLFLAHLYFENDTSGLF
                     FRLVRSTTPPGKPINVIATRRPYDDPKVARIYYRLEPVRTTILAKTHMPYLLNTQRME
                     RYHTLFLEPEYDVPTLPSYDPEASANPFETFERLPVKSRYQFLLDEAEFTLMNFIKGP
                     VCRGQIALNVINEHFWIVFGNPDSPALAQNSKFLEKEQRNLRMPTEQQSNAGALRNWL
                     EYSKLEKKYFAGKVEYLQQHLSTPEEINLDLIWNGDGWNTNAALTVFRHLDNATVVKG
                     FVGDEPKTLVLLDYALLERIHYLLVAGYDIYGNIGHQLNSRLYMDFLRMEGELGFLTL
                     LPERTRADEWKFWYRDASSEIENFGEVYNQRVNRETGIHFTTAHPKSELVEMLRQHLK
                     PVLSPSYALEEIDNTHVRQQLKRLARIQGVAVSWLPQNIIVSISDSGSTDPGDSSNQV
                     VTLIHNNGLSNVSHLFFEQERRIP"
BASE COUNT          561 a          555 c          603 g          594 t
ORIGIN      
        1 cgggaatccg tctttcttgt tcaaagaaca gatgcgacac attgcttaag ccattgttat
       61 ggatgagggt caccacctga ttgctcgaat cacctggatc cgttgatccg ctgtcactga
      121 tcgagacgat gatgttttgt ggtaaccagg aaacggcgac accttggatg cgcgcgagtc
      181 ttttcaattg ttgacggacg tgcgtattgt cgatttcctc gagggcataa gagggggata
      241 ataccggctt gaggtgttgg cgtaacatct cgaccaattc ggatttgggg tgggcggtgg
      301 tgaagtgaat gcctgtttcg cgatttactc gttggttgta cacctcacca aaattttcaa
      361 tttcagaact ggcgtctcga taccagaatt tccattcgtc tgcccgcgtg cgctcgggga
      421 ggagggtgag aaaacccagc tcaccttcca tccgcaaaaa atccatatag agccgtgagt
      481 ttaactggtg accgatattg ccataaatgt catatcccgc gaccagcagg tagtggatgc
      541 gctcaagcaa cgcataatcg aggagcacca atgttttggg ttcgtcgccg acaaagccct
      601 tgacgaccgt ggcattatct aaatgccgaa ataccgtgag ggctgcattg gtattccagc
      661 cgtcaccatt ccaaatgagg tcgagattaa tttcttccgg tgtggagaga tgttgttgca
      721 gatactcaac ttttcctgcg aaatatttct tttccaattt agaatactcc aaccagtttc
      781 tcagggctcc agcattactc tgctgttctg tcggcatacg aagattccgc tgttccttct
      841 caagaaattt ggaattctgg gctagagccg gactatcagg attaccgaac acaatccaga
      901 agtgctcgtt tatcacgttg agagcgattt gtcctcgaca tacgggccct ttgatgaaat
      961 tcataagggt aaactcggct tcatcaagca ggaactgata ccgggatttg accgggaggc
     1021 gttcaaacgt ttcgaaggga ttggcagagg cctccgggtc ataggacggg agagtgggaa
     1081 cgtcgtattc cggttctaaa aagagcgtgt gataacgctc catgcgctgc gtgttgagca
     1141 aatagggcat gtgggttttg gccaaaatgg ttgtccgaac cggctccaaa cgataataga
     1201 tgcgtgccac cttcggatca tcgtagggac gacgcgtggc aatgacgttg atcggtttcc
     1261 ctggaggagt ggtcgatcga accaggcgaa aaaataatcc ggaggtgtca ttttcaaaat
     1321 acagatgcgc caggaataaa tgctcgtaca ggtaacgact cattaactgt tcttttagac
     1381 tattcccatt gaaaaacgtt tcccacttcg gaatgtttgc cagagggctt gctcccaatg
     1441 gaggtggagt tgcatagccc gcgccttctt ccaaccaggt cctcaaggtc ctgtattccc
     1501 cgtctgacaa atccggtaag ccatagggca tcccccataa gggatgggtc ttggcaaagt
     1561 catcaaattc ttccagcatt gggcaatatt gggcacgctc aagggaaaaa tcaaagctgg
     1621 ctggtagggg gctgaccgtg ggaagggggt gctgtcgctt cagctccaac attttggcca
     1681 tcaagccccc attgaggttg gcttcttttg tctgcacacg ttcgtttaac accggataaa
     1741 accgtttctc ccgccattca gccggggaat gggcatcgac aaaaagccgc gtcggttcca
     1801 tggccagtaa tcttccggca tgataaacca gatccttatt ggcaccgcgt tcgatccctt
     1861 caggtgcact gaggtcgagt tggcaaggag catcgtaaca gccatgacac acgacacacc
     1921 ggttctcaag aatgggtttc acatcctcca catacgtgac tgttcgtacc tggaccccgg
     1981 gtgtagtggc cagccggttt tggggcgagg gcttcccgaa ttgttgatcg agctgtagcc
     2041 cgaccaggag ggtacacccg ccgccgatga ccatgagaag cagggtggaa accattcgtg
     2101 cgtatggtcg agagttcatc gtatctccaa aggggtaatc tttaagtgct taaccccatt
     2161 tttaattgtt tgatcgattc ccactatatc gaggataggg tgaaaattgc taacctcgct
     2221 atatgactat cctcgcaaaa gttttcacgg tacatttctg gaaacccttc tagcattgga
     2281 gaggcgtcta tgatgttgac ccacgtaaga tcc
//
LOCUS       DATIAL010000494         4630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1272276, whole genome shotgun sequence.
ACCESSION   DATIAL010000494 DATIAL010000000
VERSION     DATIAL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272276"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..506
                     /locus_tag="VLA60_15215"
     CDS             264..506
                     /locus_tag="VLA60_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HSF10759.1"
                     /translation="MTYTTLRANLASAMDRVCNDHEPLIITRNGEQSVVMLSLEDYKA
                     LEETAYLLRTPANAKRLLSAAAQLNAGKGVERKLAK"
     gene            503..757
                     /locus_tag="VLA60_15220"
     CDS             503..757
                     /locus_tag="VLA60_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HSF10760.1"
                     /translation="MKLTFADEGWEDYLYWQKQDKPMVERINKLIRETQREPFTGIGK
                     PEPLKHALSGFWSRRITDEHRMVYRIEGDALLIAQLRFHY"
     gene            complement(821..1603)
                     /gene="mtgA"
                     /locus_tag="VLA60_15225"
     CDS             complement(821..1603)
                     /gene="mtgA"
                     /locus_tag="VLA60_15225"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HSF10761.1"
                     /translation="MKRSRPKAAKTRRPKPLWNIGSQRKPRVRRFRWIRIIPFKAIFI
                     LLGLYVLFEIFTFPFLSIVRLPTENPTESALMRQRIDEAHAQGTTLKIDYRWTPLSSI
                     PRHVRMAILVSEDGTFYSHTGVDWHEVLESIETNLEKGRIVRGGSTITQQLAKNLYLS
                     TSRDPIRKGKELLITWLLESTLSKKRILELYLNIIEWGPGVFGIEAAAQRYFHKPASR
                     LSMDEGARLAAVIPSPLRHQPTETTSYVEKKKELILRRMSVR"
     gene            2024..3028
                     /locus_tag="VLA60_15230"
     CDS             2024..3028
                     /locus_tag="VLA60_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HSF10762.1"
                     /translation="MMNAMILEQDADVATGPLRARDIPKPEPGPGQVRVKVHVCGVCR
                     TDLHVVEGELPPSTRPIVPGHETVGIVDRVGQGVKQIKEGDRVGIAWLQGTCGACEFC
                     KAGRENLCAQATFTGYHVNGGYAEYALVPEQFAYPIPSVFTDEEAAPLLCAGIIGFRA
                     LRRSQVKPGQRLGLYGFGGSAHITIQVARHWGCEVYVCSLREEHRTLARQLGAVWVGT
                     ASEMPPNSLHSAIMFAPAGELVPPALRALEKGGTLALAGIYMTPIPSLDYTLDLFQER
                     TLQSVTANTRQDGLDLLEAAAAIPIHTHTVPFQLEEANLALQQLKAGTIQGAGVLHVM
                     "
     gene            complement(3033..3479)
                     /locus_tag="VLA60_15235"
     CDS             complement(3033..3479)
                     /locus_tag="VLA60_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10763.1"
                     /translation="MLVREVMSTGVLTAFPTDSVRTVVIKMLSRHCGAIPVIDQQENL
                     IGLVALRDVILPLFPNFGDYIHDNVHSRDFTEMEEGYPEVLKKKVEDVMSRNPLTVSP
                     STPILEAASYMGVKNFRRIPVAENGKLMGMVSIGDINRGLFFERGC"
     gene            complement(3546..>4630)
                     /gene="ggt"
                     /locus_tag="VLA60_15240"
     CDS             complement(3546..>4630)
                     /gene="ggt"
                     /locus_tag="VLA60_15240"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HSF10764.1"
                     /translation="DRLIQNDLAWSLQQISEQGAGAFYDGPIAERVSAEMRSHGGLMT
                     PEDLSRYRAVWRQPLRGTYRGYDIVSMPPPSSGGVHLIQMLKILEHEPLKTFGHNSAR
                     TIHWLVESMKLAYADRSRWLGDPEFVEVPVHGLISRAYAKQLHASIDPQKARPSSSIR
                     PSNPLPYEGRDTTHFSVMDEDGNVVSNTYTINFSFGSGIVAEGTGILLNNEMDDFVAK
                     PGTPNAYGLVGSAANAIEPGKRPLSSMTPTLVLHNKKPFLATGSPGGSHIITTTLQLI
                     LNVIDHDMNLASATAAPRVHHQWMPDEVRVERGLSPDTLILLEQWGHTITVGESMGGA
                     QSILKGPQGFYGASDPRKPDSLAAGY"
BASE COUNT         1101 a         1252 c         1210 g         1067 t
ORIGIN      
        1 cctttttctt ttccacatag gaagtcgttt cggtaggttg gtgccgaagg gggctggaaa
       61 ggaccgtcgc taaacggatg cccttctcca tcgaaagttg agccggtaaa acagaaggaa
      121 aaacgaaatc tgtaggagca cgcctggatt ctgtcagagc ctgggttatt cccgatagac
      181 caaccgaatt gtcactctag acaaactttt cttctgactg tacagtaatc tgtacatata
      241 ggaggaacat aactctggat gccgtgacgt atactactct tcgtgcaaac ctggccagtg
      301 ccatggaccg ggtgtgcaac gaccatgaac ctctgatcat cacccgcaac ggagagcagt
      361 ctgtcgtgat gctttcgctg gaagattata aggcgttaga ggaaaccgcc tatctcttgc
      421 gtacgcccgc caacgccaaa cgcctccttt cggccgccgc ccaattgaac gccggcaaag
      481 gtgttgagcg gaagttggcc aagtgaagct gacatttgct gacgaaggat gggaagatta
      541 tctctattgg cagaagcagg acaaacccat ggtcgaacga atcaacaaat tgatacggga
      601 gacacaacgc gaacccttca caggaatagg taaacccgaa ccattgaagc atgcactttc
      661 agggttctgg tcccgccgaa taactgatga gcatcgcatg gtctatcgaa tagagggaga
      721 cgcactcttg atcgcccagt tgcgcttcca ctactgaaaa accccccgtc agagtccatg
      781 gtgtgccttc cccacggtag tttcgcaaat tcttgtctag ttatcgcact gacatccgac
      841 ggaggatcag ctcttttttc ttttccacat aggatgtggt ctccgtgggc tggtgtcgaa
      901 ggggactggg aatgaccgct gccaggcggg cgccctcatc catagaaagc cgcgaggccg
      961 gcttgtggaa ataacgttgg gcggcggcct caatgccaaa cacgcctggc ccccattcaa
     1021 tgatattgag gtagagttcc aggatacgtt ttttactcaa ggtcgattcc aacagccaag
     1081 tgatgaggag ttctttgcct ttgcgaatcg gatcccggga ggtcgagagg tataagttct
     1141 tggccaattg ctgggttatg gtcgagcccc cccgaacaat tcgtcctttc tccaaattgg
     1201 tttcaatcga ttccaggacc tcatgccaat ccacgccggt atgagaataa aatgtaccat
     1261 cctcggatac cagaatggcc atacgaacgt gacgcggaat gcttgagagc ggcgtccatc
     1321 ggtaatcgat tttcagcgtg gtgccttgag cgtgtgcttc gtcgatgcgt tgccgcatca
     1381 gcgcggactc tgtgggattc tccgtgggca accggacgat cgagagaaac ggaaaggtga
     1441 aaatctcaaa taacacataa aggccgagaa gaataaatat cgctttaaat ggaatgatgc
     1501 gaatccaccg gaatcgccga acacgaggct ttctctgcga accgatattc cagagaggtt
     1561 tggggcgacg ggtttttgcg gctttcggcc ttgatcgctt catgctgtgt tcttgactgc
     1621 ccttcacctg tccctgcgga tacgatatga tgaggccctg gtggacagaa ccgactcgat
     1681 gttgcccgaa gtacgtatca atttagcgag acagttgtcc aatgattttt cgaaacagtg
     1741 ggatcccatc taacacaaac acgagtaagg taaaactcgg accggagtgg gttttatgat
     1801 atcctggctc tacccgggca cagattcttc gccattggct tagaacgaca actctgccgt
     1861 catcaccggg tcggtggccg ggatctatcc gaaataggat cacaatgaat tcccgatcac
     1921 ccatatccga aatgactatg atagagatgg atgaagaaca tgggcctcac ctgtttgctt
     1981 aatacctgaa tgggataatc tacatttcac cgcataggag tgcatgatga atgccatgat
     2041 cctcgaacag gacgccgatg tcgcaacggg tcctttacgg gctcgcgata tccccaaacc
     2101 tgagccgggt cccgggcaag ttcgcgtcaa ggttcatgtc tgcggagtgt gccgcactga
     2161 tctgcacgtc gtcgaaggcg aactgccccc gtctacgcgt cccattgtcc ctggacatga
     2221 gaccgtcggc atcgtggatc gagtgggaca aggagtcaaa cagatcaaag aaggtgatcg
     2281 agtaggaatc gcctggctcc agggaacctg cggagcctgt gaattctgca aggcagggcg
     2341 ggaaaatctg tgcgcacagg ccaccttcac cggttatcac gttaacggtg ggtatgccga
     2401 atacgcactg gtccctgagc aattcgccta ccctattcca tccgtgttta cggacgaaga
     2461 ggccgccccc ctattgtgtg ccggcatcat cggctttcgt gccttacgcc gcagtcaggt
     2521 gaaacccgga cagcgactgg gcttgtacgg attcggtggc tccgcccata ttacgattca
     2581 agtcgcccgg cattggggtt gtgaagtcta cgtgtgttcc ttacgggaag aacatcgcac
     2641 cctggctcgt caactgggag cggtgtgggt cgggacggcc tcagagatgc caccaaactc
     2701 tcttcactca gcgatcatgt ttgccccggc gggtgaactg gtgcctccgg cactacgggc
     2761 attggaaaaa ggcggcacac tcgccttggc cggaatctat atgacaccca ttccatcctt
     2821 ggactataca ctcgatctgt ttcaggagcg gacactccaa agcgtcaccg ccaacacccg
     2881 ccaggatgga ttggacctgt tggaggccgc cgctgccatt cccattcata cccacacggt
     2941 tccctttcaa ttagaagaag ccaatctggc actccaacaa ttaaaggccg gaaccatcca
     3001 gggcgccgga gtcctccatg tgatgtaaag gcctagcacc ctcgctcgaa aaacaggccg
     3061 cgattgatat cgccgatgct gaccattccc atcagtttgc cgttttccgc gacggggatt
     3121 ctcctgaaat tcttcacacc catgtaggaa gcggcctcta atatcggcgt ggagggcgag
     3181 acggtcaaag gattccggct catcacatct tcaacctttt ttttcagaac ttcgggatat
     3241 ccttcctcca tctcagtgaa atcccggctg tgaacattgt catggatata gtcaccaaaa
     3301 ttcgggaaaa gtggtaagat gacgtctcga agcgccacca acccgatcag attttcttgt
     3361 tgatcaatga ccgggatcgc cccgcaatgc cggctcaaca ttttgatcac aaccgttcga
     3421 accgagtccg tcggaaacgc cgtcaacact cctgtggaca tcacttcacg tacaagcata
     3481 cccacctcct gcgttgaggg tgattgaggt aaatactgta ctcccctcaa attgacattg
     3541 tcagcttagt acccggcggc cagggagtcc ggtttgcgag gatcagatgc cccatagaat
     3601 ccctgtggcc ccttaagaat gctttgagcc ccgcccatag attccccgac cgtgatggtg
     3661 tgaccccatt gttcaagaag gatcaaggtg tccggactca atccgcgttc cactcgcact
     3721 tcgtcgggca tccattgatg atggacacgg ggcgctgcgg tggcagaggc caggttcata
     3781 tcatgatcaa tgacatttaa aataagttga agtgtcgtgg tgatgatatg acttcctccg
     3841 gggcttccgg tagccaggaa gggtttttta ttgtgtaaaa ccaaggtcgg agtcattgag
     3901 gagagagggc gctttccggg ttcaatcgca ttggccgccg aacccaccag gccgtaggca
     3961 ttgggcgttc cgggcttcgc cacaaaatca tccatttcat tgttcaacag aattccggtt
     4021 ccctcggcca caatgccgct gccaaagctg aaattgatgg tataggtgtt cgacacgaca
     4081 ttcccgtctt catccatgac cgaaaagtgt gtggtatccc gcccctcgta aggcaaaggg
     4141 ttggagggcc gaatacttga tgaaggacga gccttctgcg gatcgatgga agcatgtaac
     4201 tgtttcgcat atgccctgga gatcagaccg tgtacgggca cctcgacgaa ttcaggatcg
     4261 cccagccatc ggctccggtc cgcataagca agcttcatac tctcaaccag ccagtgaata
     4321 gtcctggcgc tattgtggcc gaaagtcttt agtggctcgt gttccaggat tttcagcatt
     4381 tgaatcaaat gaacgccccc ggaactgggc ggtggcatcg acacaatgtc atacccgcga
     4441 tacgttcctc gcagcggttg ccgccagacc gcacgatagc gactcaagtc ttctggggtc
     4501 atcaatcctc cgtgggaacg catctcggcg ctcacacgct ccgcgattgg accgtcataa
     4561 aaagcacctg ccccctgttc ggaaatttgt tgaagcgacc acgccagatc gttctggatc
     4621 aaccgatccc
//
LOCUS       DATIAL010000495         6145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1379595, whole genome shotgun sequence.
ACCESSION   DATIAL010000495 DATIAL010000000
VERSION     DATIAL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379595"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..582)
                     /locus_tag="VLA60_15245"
     CDS             complement(28..582)
                     /locus_tag="VLA60_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10765.1"
                     /translation="MRSFILPIFRFTSYVCKRGVLAAGLVLCLVYGVSAQEVTVSEDT
                     FGCILDWPKVRNTRIKHDDPEKLKEAKRILRDSVPETDYPVGTILQLVPHEAMVKHSH
                     DKFPKTNGWEFFFLEVSKEGTKIADRGDNVVNVSQGVTCLSCHQPAARFDFVCEKGHG
                     CAPIPFDDQKIAAIQQADPRCTKN"
     gene            complement(809..1252)
                     /locus_tag="VLA60_15250"
     CDS             complement(809..1252)
                     /locus_tag="VLA60_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10766.1"
                     /translation="MKSAIQKHGFILLFVLLLFSFSSVSYGGNECKIKYGWNTGNSLQ
                     GTFKNHFNTIYLNTGETKTINQNRMNYVKNLTSQEVKFYLENASDVTLGKNQQNPDVG
                     TYVLAVKLVKAKCLPTSSSSPSFPDVSKTPSPTGPIPIPYPNIGK"
     gene            complement(1833..2063)
                     /locus_tag="VLA60_15255"
     CDS             complement(1833..2063)
                     /locus_tag="VLA60_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10767.1"
                     /translation="MWKPGKKEGPGPRELVRLAVPRPVYTQSHVDYIVEVVEEVFRRR
                     EKLKLFRFLEQTSFLRHFAAQYEIPERSKWGN"
     gene            2283..2417
                     /locus_tag="VLA60_15260"
     CDS             2283..2417
                     /locus_tag="VLA60_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10768.1"
                     /translation="MSELVETALRLLFQNQKVPKPLSPLPTFKSGGYLVDIANREAPY
                     "
     gene            complement(2517..3896)
                     /locus_tag="VLA60_15265"
     CDS             complement(2517..3896)
                     /locus_tag="VLA60_15265"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HSF10769.1"
                     /translation="MFKTIIEPFRIKMVEHIPMTTETERRELIAKAGYNVFRLHSDDV
                     LIDLLTDSGTGAMSAKQWAAIMRGDESYAGSPSFYRFEAAVQELMGFPLVIPTHQGRA
                     AERILFTTICKAGDVVPNNTHFDTTRANVEFCGARAVDVPCRELADLSSEFPFKGNMD
                     VERLRAVFQKEGRERIPLVMITVTNNSGGGQPVSMANMREVAEVCRAFERPLYLDACR
                     IAENAYFIKLREPGYADKLVRDIVREMCDLADGCTMSAKKDAIVNIGGFLATRNAELA
                     QQEQNLLILTEGFPTYGGLAGRDLEAMAVGLHEGMEEDYLQYRITSTGYLGNHLHEAG
                     VPILRPPGGHAIYLDAARFAPHLQPLDLPGISLCVELYVLGGIRAVEIGTAMFGKRNP
                     ETGKEVPGPRELVRLAIPRRVYTQSHVDYIVEVVEEAFRRREGLKPFRFLEQAPFLRH
                     FTAQYGMVE"
     gene            complement(4244..6070)
                     /gene="ftsH"
                     /locus_tag="VLA60_15270"
     CDS             complement(4244..6070)
                     /gene="ftsH"
                     /locus_tag="VLA60_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HSF10770.1"
                     /translation="MEKKYRVSIWYFIIAFWGLIILQEMYFAAQHMDEVPYSQFKTWV
                     QDDKVAEISITDKVIHGKLKSEKGGESPQWFQTVRVDDPELVKLLEEKHVEYAGVIVS
                     TLWKDVASWVVPILVFAGIWFLILRKMGQGAGGGFMRIGKSKAKVYIESDIKTRMDDV
                     AGVDEAKVELMEVVEFLKTPEKFTRIGGRIPKGVLLVGPPGTGKTMLAKAIAGEAGVP
                     FFSISGSEFVEMFVGVGAARVRDLFEQAMAKAPCIIFIDELDALGKARGTGPMMHEER
                     EQTLNQLLVEMDGFDPRVGVIMLAATNRPEILDQALLRAGRFDRQVLVDRPDRPGRAA
                     ILAIHAKSIKLAPDVDLDKIAAMTPGMVGADLANVVNEAALLAIRRSRETAEHRDFEE
                     AVERVIAGLEKRNRVLSVEERKRVAHHEVGHALVAMSLPGCDPVQKISIIPRGIAALG
                     YTLQLPIEDRYLLTRSELENRIAVLLGGRMAEELVFGEASTGAADDLQKATTIAKRMV
                     KDYGMSDKLGTVALDESVQPTFLKNMESHATPTYSEHTAQQVDQEVRRLIEEQGIRVR
                     ELLTKLRPVLLNGAETLLKAEVMTGEELMALLHAKEEEPIPG"
BASE COUNT         1492 a         1698 c         1378 g         1577 t
ORIGIN      
        1 tataaatgac ctcaaaactt tccccaccta gtttttggtg cagcgggggt ctgcttgttg
       61 aatggccgcg atcttttgat catcgaacgg gattggagcg cagccatgtc ccttttcaca
      121 gacgaagtcg aacctggccg ccggctgatg acaacttaag cacgtgaccc cttgcgatac
      181 attgacgacg ttgtctcccc gatccgcaat cttggtgcct tctttggaga cctccaaaaa
      241 gaaaaattcc cagccgtttg ttttgggaaa cttgtcatgc gaatgcttca ccatggcttc
      301 atgaggaaca agttgcagga tggttccaac cggatagtcg gtctccggca cgctgtcccg
      361 taagatacgc ttggcctcct tcaatttttc cggatcgtca tgcttgatgc gcgtattcct
      421 gactttcggc caatcgagaa tgcaaccgaa cgtgtcttcg gacaccgtca cttcttgagc
      481 cgacacgcca tacaccagac aaagcacgag tcccgcagcc agaactcccc gcttacacac
      541 atacgatgtg aatcggaaaa tgggcaaaat gaaggaacgc atgaccatac tcctttcttt
      601 ttgttttccc ccctgaacaa gcgctctgga taaaatacaa ggggtcacag agatttacct
      661 catcgtgccg taactccgcg cgcgcctact ggctgtataa atcaattctc ttgcctgccc
      721 cctttttgcc aaaggaataa ggaaaggttg atttctagaa ctggtcttag cagaaatgcc
      781 tcattcttgt tcccctttta tgtgtaactc attttccgat attcggataa gggatgggta
      841 tgggaccagt cggagaggga gtcttactta catctggaaa agatggtgaa ctactcgatg
      901 tgggcaagca tttcgccttc accagtttaa ctgcaagcac ataggtgcca acatccgggt
      961 tctgctgatt tttccccaat gtcacatcgg aggcattctc taaataaaac tttacctctt
     1021 gggaggtcag attttttacg taattcatac gattttgatt gatcgttttg gtctcccctg
     1081 tgttcagata gatggtgtta aaatggttct taaacgtacc ttgtagagaa tttccggtat
     1141 tccagccata tttaatttta cattcgttcc caccatatga aaccgatgaa aaagaaaaca
     1201 ataacaacac aaataagaga atgaatccat gtttctggat tgccgatttc atttgcatat
     1261 ccctttctct ctggtaagga ttgaaagttc caacgttact tccatgggtg gtatgtcgat
     1321 ggaactgatt ctttggttca aatgaagccc tctggacatc cccgtatgat caaaatcgat
     1381 attcaatctc cccgcccact cccatccatg gaggtgccgc gaaagtccat cacccactgt
     1441 ttgacgttct ttccctcata ggccgttgcc atcattcatc ttccagagaa ccaccataca
     1501 aaattttgtg aaaaatggga atgtgatgaa tcgaggctgg gcattaatgg gtcgtcctag
     1561 gcgcaacctt ttcgccccat tcaaacaatg gcaattgttt tcctggggtg agattgtaca
     1621 tcaatactca taactgaccc attttttttc gtcccgcctg cgccgtgcga cccgctcaga
     1681 tctagtgaat gaaatagccg cactccggaa aaggcattca atcccaacat ggacatgctg
     1741 ctacgaatgc aggcggggga tgatgcgacg gcaatgcgaa agcaggcaga aaaaaacccg
     1801 gcacgcactc tgtaccgtca taggtttagt tcctagttcc cccatttgga tctttccggt
     1861 atttcgtatt gggcggcgaa atgtctgaga aatgaggttt gttccaggaa gcgaaacagc
     1921 ttcaattttt cacggcgccg gaagacttct tccacgactt ctacaatata atccacatga
     1981 ctttgagtgt agaccggccg gggaacggcc aggcgcacga gttcgcgagg acccggccct
     2041 tctttctttc ccggtttcca cattgcgcct ctgccaaaca tgacggtgcc gatctccacc
     2101 acaccgacta aactgcccta tcttcatgca agaatccata gacaaaggct cctgcccccg
     2161 gccgtcacca actctcttct cccgacccat tttttttagg gtttttcaag tccattgtta
     2221 aaggatcgat ttattggctc ttagcccaac tcaaacgaga agctgcaaca caaggacgga
     2281 cgatgtcaga attagtcgaa accgcccttc gattgctatt tcaaaaccag aaggtcccaa
     2341 aaccactttc tccgctgccc acttttaaaa gcggcggcta tctggtcgat attgcgaatc
     2401 gggaagctcc ctattaggac atggaaggtc cctagagttt tcagaacaac atcttccatt
     2461 ttcggattct caacctcttg ggcttccctg aatgacttcg taaatcttta tataaattat
     2521 tccaccattc catattgcgc ggtgaaatgt cggagaaagg gcgcttgctc cagaaagcga
     2581 aatggcttca agccctcacg ccgccggaac gcttcttcca ccacttccac gatataatcc
     2641 acatggcttt gggtgtagac ccgcctggga atcgccaacc gaacaagttc gcgagggccg
     2701 ggcacttctt ttccggtttc cggattacgt ttgccgaaca tcgcagtacc gatttctacc
     2761 gcccgaatgc ctcccaacac gtataactcc acacagagcg aaatacccgg cagatcgagc
     2821 ggttggagat gcggggcgaa gcgggccgca tccagataaa tggcatgtcc acccggcggc
     2881 cgcaggatcg gcacaccagc ttcatgtaaa tggttgccca gataccccgt ggaggtaatg
     2941 cgatactgga gataatcttc ctccatgcct tcatgtaacc ctacggccat ggcttccaaa
     3001 tcccgtccgg ccaatccccc ataggtggga aacccctcgg ttaagatcaa caagttttgc
     3061 tcttgttggg ccaactcggc gttgcgtgtg gcaagaaatc cgccgatatt cacaatggca
     3121 tccttttttg cggacatggt acagccatcg gccaggtcac acatttcgcg aacaatgtcg
     3181 cggaccaatt tgtctgcata tcccggttcg cgtagtttaa tgaaataggc attttctgca
     3241 atccggcagg catccaggta caagggacgc tcaaaggccc ggcagacctc ggccacttct
     3301 ctcatgttgg ccatcgaaac cggttggcct ccaccggaat tattggtgac ggtgatcatc
     3361 accaacggaa tacgctcgcg cccttctttt tgaaaaaccg cgcgaagcct ttcgacatcc
     3421 atattgcctt tgaagggaaa ctcactggag agatcggcca attcgcgaca gggcacatct
     3481 accgcccggg ctccgcaaaa ttccacgttg gccctggtgg tatcgaagtg cgtattattg
     3541 ggaaccacgt caccggcctt gcagatagtg gtaaacaaga tgcgttcagc cgcccttccc
     3601 tgatgggtcg gtatgacaag cgggaatccc atgagttcct gtaccgcagc ttcaaaccgg
     3661 taaaaggatg gacttcccgc ataactttca tccccccgca tgatagctgc ccactgtttg
     3721 gcactcatgg ccccggtgcc gctatcggtc agaagatcga tcaggacatc atcagagtgc
     3781 agccgaaaaa cattgtaacc ggctttggca attaattccc ggcgttcggt ttctgtcgtc
     3841 atcgggatat gttcgaccat tttgatacga aagggttcga tgatcgtctt aaacatacgc
     3901 ggtcctctgg ttatactgtt cgctttattt cacaatcggg caaaagacca tgattcccgg
     3961 tcaccgaaat catatcatct tctggaaaca catcgaagga ttcatgactt gacgattcgc
     4021 gcctctattg catgaaacac tgaggctaac cggctttctt gaggaatggg gtgagaagga
     4081 ttcctgatct gccctccccg aacaggacga ggagaccgac actgcgggat gttttggaat
     4141 ggggttttac ctgccaggtc tgaacttcag gcctggcagg aattaattgg agctttcagg
     4201 atggagggat tcggtaacga aattcaaatg ttttcggatg gaactagcct ggaatgggtt
     4261 cttcttcctt agcgtggaga agcgccatca actcttcccc ggtcatgact tcggccttca
     4321 acaaggtctc agccccgttc aacaagaccg gccgaagctt tgtcagcaat tccctgacgc
     4381 gaatcccttg ttcttcaatg aggcggcgca cctcttgatc gacctgttgc gcggtatgct
     4441 cggaataggt cggggtcgca tgcgactcca tattttttaa aaacgtcggc tgcacggatt
     4501 catctaacgc caccgttcct aatttatcgc tcatcccata atctttgacc atccgtttgg
     4561 cgatggtggt cgctttctgc aaatcatcag ccgctcccgt ggaggcttct ccaaagacca
     4621 actcctccgc catacgcccc cccaacaaca cggcaatgcg attttctaat tcggaacgag
     4681 tgagtaaata ccggtcctcg atcgggagtt gtagcgtata gcccaaggct gcaatgccac
     4741 ggggaatgat cgagattttt tggactgggt cacatccggg caaggacatc gccactaacg
     4801 cgtgaccgac ctcatgatgg gccactcgct ttcgttcttc cacactgagg acgcggtttc
     4861 tcttttccaa accggcaatg acacgttcca cggcttcttc aaaatcccgg tgttccgccg
     4921 tctcccgact tcgtcgaatg gccagtaagg cagcttcatt caccacattg gctaggtcgg
     4981 ccccaaccat tcccggagtc atggcggcaa ttttgtctaa atcgacgtca ggagccagtt
     5041 tgatggattt ggcatgtata gccaaaatag cggctcttcc aggccgatcg gggcgatcaa
     5101 ccagcacctg ccgatcaaaa cggcctgcac ggagcaaggc ctgatccaga atttcaggac
     5161 gattggttgc ggccagcata atgaccccca cacgaggatc aaacccatcc atttccacca
     5221 ggagttgatt cagggtttgc tcccgttctt cgtgcatcat gggccctgtt ccccgggctt
     5281 ttcccagtgc atccaactca tcaatgaaaa tgatacaggg cgctttagcc atggcttgtt
     5341 caaacaaatc tcgcacacgg gccgctccca cccccacaaa catttcgaca aattccgacc
     5401 cgctaatgga aaaaaacggc acgccggctt ctcccgcaat ggctttcgcc aacatggttt
     5461 ttcccgtgcc cggcggcccc actaagagca cccctttggg gatccggcct ccgatcctgg
     5521 tgaatttttc cggagtcttc aaaaattcaa ccacctccat gagttccact ttggcttcat
     5581 cgactccggc cacatcatcc atgcgggttt tgatatcgct ttcaatatag actttcgctt
     5641 tagatttccc gattcgcata aaaccgccac ccgccccttg tcccattttc cgcaaaatca
     5701 aaaaccaaat cccggcaaag accagaatag ggacgaccca ggaggccacg tctttccaga
     5761 gcgtactgac gatcactccg gcatattcca catgcttttc ttccaacagt ttcactaatt
     5821 ccgggtcatc tacgcgcact gtctgaaacc actggggact ttccccgcct ttttcagatt
     5881 ttaattttcc gtgaatgact ttgtcggtta tggagatctc ggccacctta tcatcttgaa
     5941 cccaggtttt aaattgacta taaggcacct catccatatg ttgggccgca aaatacattt
     6001 cctggaggat aatgagtccc cagaacgcaa taatgaaata ccaaatagag acacgatatt
     6061 ttttttccat atttgctatc ctaacccctg ttcctgcgtg accctgaatc tcatgacgtg
     6121 aacataatct ttactgtacc caaga
//
LOCUS       DATIAL010000496        13373 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1595896, whole genome shotgun sequence.
ACCESSION   DATIAL010000496 DATIAL010000000
VERSION     DATIAL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13373
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595896"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VLA60_15275"
     CDS             <1..1161
                     /locus_tag="VLA60_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10771.1"
                     /translation="VLFTGTDPSIPQEFLIPEDFRVEVSQATDLQLVQTVSQLIAAYT
                     DQLLFSQDDEGCFNLSPYDVFLEINSLPRQPDKWESPSSYARRLLQQIKFLESYDSQN
                     YFRWPSTPFHDSPTLRFVEKNPHTADGKFQFHDQPFIFGPQELQGLKIFFAQQGRHKH
                     SHKGHQIRPSDLAQGGIGNCIACHTPPNFTDFRFHNTGIAQTEYERIHGPGTFFILDI
                     PGLHQRNKNIEAYLPATAKHPHAQEPFRAIPAPDNPQWTDLGIWNIFWNPDFPESQLP
                     IWSILCEDALNEHFGVFRGFQACRPDRLLPRAIGAFKTSGLRDLSHSAPYSHAGIADT
                     LEDVIQGYITNSDLARKGMLRNGDSHLKNIALIQEDIPALTAFIRSLNEDYE"
     gene            4915..6084
                     /locus_tag="VLA60_15280"
     CDS             4915..6084
                     /locus_tag="VLA60_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HSF10772.1"
                     /translation="MIAPASALSPVSKTPFLSIHQWLYLFLCLMVGMYAGLGHAPSQW
                     TDALPDIILWGATGLAVGCLALWLERHLTACHRSTLIGGSLGLIVALAGIGFVLNMGM
                     GTGLLQMSSLTPWIGLPIFLLCPYIGLMIGIHISNSLSPTSIAELPKESPPPTSQPPS
                     KTVQKLLDSSAIIDGRILPLCTTGFLEGPFIVPKSILHELQTLADSAHLSKRTKGKRG
                     LDILSQLQQLPNMDVVIIEDWEPDISAVDHQLIAIAKNREAKIVTNDWNLAKVAALQG
                     VPSLNVNELTYQLRPLVLPGETIRVFIHKEGQGQEQGIAHLDDGTMVVVDHGATLVGQ
                     AIEVVVTRFMQTNTGRMIFSTPQSKHSPLPVNFQTALKNSEVVSGYSRSLVKDHG"
     gene            6821..6994
                     /locus_tag="VLA60_15285"
     CDS             6821..6994
                     /locus_tag="VLA60_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10773.1"
                     /translation="MPKPGEFEEDVRQCRQSLISNPDDRGISGTLSRVFKLSEDAVGQ
                     CLMAKGWFLAEKP"
     gene            complement(7066..9804)
                     /gene="glnD"
                     /locus_tag="VLA60_15290"
     CDS             complement(7066..9804)
                     /gene="glnD"
                     /locus_tag="VLA60_15290"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HSF10774.1"
                     /translation="MTCLVLRELPSMSEGMSLPPTGLTEHKDSSLGLHVLQSQREVLR
                     QRLIEGATGSEITQAFSDFMDALLIARFREVIQQGNAGVRAGWQQCCLVAMGGYGRRE
                     LAPYSDIDVMILTQGNQREVAEALSTGVFHRLWDLGFQVGHSVRSLVECLTIAEADLA
                     ACTSLMESRFLIGSASVFQQFQRKFERRIIKKRAQRFVLDKIQEREREYAKFGETVFM
                     LEPNIKKSKGGLRDLHLLQWVGQARYGAGTIQELSNRGIIAIQDYQVLQEAQEFLWRV
                     RCFLHFEAGRAQDILTFDDQVRLSALWGFVDHSHLLAVEQFMQVYFRHTTGLFDRGRR
                     FLDQAREESWLDRVRQWWPPPLIEGHFRIMENRLTIQSDKLLEVMESPMLILSLFVTS
                     QRQAVPIDSHILNELSQHMGTLPNERFHTKPVSRLFRHILSSPGRIAQILEAMHQASL
                     LEKLVPAFSRVRGLMQFNQYHKYTVDEHSLLAVREAERLHNHQGIIGEVYRNIADKDI
                     LHLALLLHDLGKGRPGDHSEVGQEIAHLTADRLDLSEKERQTLAFLVRHHLQMSHTAF
                     RRDLNDPKTIVTFARLVKTVGMLQKLFILTIADISAVGPEVMTKWKESLLGELYSLTY
                     AELAGDAGPSYSGDGSKNLPPEARDKLIKILRADRSEGHSPEPDWSEWVNAQLQQLPS
                     RYVQSTPVDQMAAYLLAIRSLPVKPTHVEARFNQASGVSEYVLITREQTKPGIFMQVA
                     GVLAAMGLQVLEAQIMTLKDGTVWDVFSVHDSDYEGPPSAGRLEEVAKEIQEVVEGRQ
                     SVHQLVERRRRMAFGRPFPTGRHPTEVHIDHGVSDAFTVIDVFADDKQGLLFVIAKTL
                     VQLGLSIHMARIGTRLDQVADVFYVTNSQGEKILSGEDCQQIQHALHAAVDQFLDE"
     gene            complement(9814..11604)
                     /locus_tag="VLA60_15295"
     CDS             complement(9814..11604)
                     /locus_tag="VLA60_15295"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HSF10775.1"
                     /translation="MMIRLAMVQMNAVVGDLEGNTRTICGWIREARKAKADVVVFPEL
                     AVCGYPPEDLLLMPKFLLDISRMRDRITKVTTGIMAVVGSVIESDRLAQHPLRDHGQD
                     RRKPLNAAWVLVNGQVKRSYAKCKLPNYGVFDEERYFQPGRTSPVYCLGPLRIGINIC
                     EDIWFADGPASRQAAYGGAHLVLNINASPYHVGKTQNRERMLAIRAKANNVMVSYTNM
                     VGGQDELVFDGNSLVMDSTGVVLARAKAFEEDFLLTDLQIPSTRKRKSKGTEKVSKVD
                     GEDVRVPMIRMGWAPNWSKSGIPGVRRLTPVIGESEEIYRALVLGVQDYVRKNAFSRV
                     LVGISGGIDSALTALIARDALGASNVMGVMMPSPYTSRESREDARQLGKKLGIEMRHL
                     SITRAFKVLLRVLGKSFQGFQADTTEENLQARIRGTLLMALSNKFGYLVLTTGNKSEM
                     SVGYATLYGDMAGGFAVIKDIPKTKVYELSRWRSEYKGSNFDQVIIPERIQVRAPSAE
                     LRPDQTDQDTLPPYPVLDAILQAYVEDNESMGRIVKMGYDTHVVRKVMGMVDRSEYKR
                     RQAPVGIKITSRALGKDRRMPITNRYFWGA"
     gene            complement(11779..13032)
                     /locus_tag="VLA60_15300"
     CDS             complement(11779..13032)
                     /locus_tag="VLA60_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HSF10776.1"
                     /translation="MATIRPSTIEDLKHLVRERTREVQILHRISESISSTLELETVLK
                     QIVEVVVEVTKGDACLLYLISETTDELILRASKNPHPRLIGRISIGLGEGITGWVARE
                     KIPVVIPRNASDDARFKFFHRLPEDRYQAFVSVPITSKGKMVGVINVQHKRSKRYAEE
                     TLALLLTIANQVGGAIANARLYDDMRQKARRLETLSQVSETVASNRLIEDVLQLIVTM
                     TAQLMNSKICSIMLVDQASGELRIAATQSLSDAYRRKPPLKVGQSMSGRAVQDQHPVY
                     VSDVKAEDGYFYRDLAKQEGLHSLLSIPMLMKENPIGVINVYTSSYHAFSDEEVTTLQ
                     AIANQSAVAIEHTRLMEKSFEMQEALEVRKLVERAKGFLMASRGLSEPEAFRLMQRQS
                     MNLRKSMREIADAIILAEELQRATQ"
     gene            complement(13034..>13373)
                     /locus_tag="VLA60_15305"
     CDS             complement(13034..>13373)
                     /locus_tag="VLA60_15305"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10777.1"
                     /translation="VGIGMVQEAAMAHFLGLCTKELVERQRDLIQDVGLPIVMPSMTF
                     MELWGAMQHDKKVVKGQINCVLPTTIGKVKVVPLVRQRIRQWFTASQSFRGKRLDPVP
                     QAVSRLSKKK"
BASE COUNT         3485 a         3645 c         2832 g         3411 t
ORIGIN      
        1 gtcctcttca ccggaaccga cccatctatt cctcaagaat tcctgatccc tgaagatttt
       61 cgcgtagaag tttctcaagc caccgaccta caacttgtcc aaaccgtttc acaacttatc
      121 gccgcctata cggaccaact ccttttctca caggatgacg agggatgttt caatctttct
      181 ccttatgatg tctttcttga aattaacagt ttgccccgtc aacccgataa gtgggagtct
      241 cccagttcat atgctcgtcg attgcttcaa cagattaaat ttctcgaatc atacgattcg
      301 caaaattatt tccgatggcc ttcaacacct tttcacgata gcccgaccct tcggtttgtt
      361 gaaaaaaatc ctcacacggc agatggcaag tttcagttcc atgaccaacc gttcattttt
      421 ggacctcagg aactacaagg gttgaaaata ttttttgctc aacagggtcg acacaagcat
      481 agtcataaag gccatcaaat tcggcccagt gacctagccc aaggaggaat tgggaattgc
      541 attgcatgtc atacaccacc aaatttcacc gacttccgtt ttcacaacac gggaattgcc
      601 caaacggagt atgaacgcat ccacgggccc ggaacttttt ttatactgga tatacctgga
      661 ttgcatcaac gaaataaaaa tatcgaagcc tacctgcctg ccacagcaaa gcatcctcat
      721 gctcaagaac cattccgggc tattcccgcg ccagacaacc cccaatggac cgatctgggc
      781 atttggaaca ttttctggaa tcctgatttc cccgaatccc agctacccat ttggtccatc
      841 ctttgcgagg acgccctcaa tgaacacttt ggggtgtttc gtggcttcca ggcctgtcgg
      901 ccggatcgac ttctaccgag agctattggc gctttcaaaa cttccggcct ccgggatttg
      961 agtcattcgg ctccctatag tcacgccggt atcgccgata cgcttgaaga tgtgattcag
     1021 gggtatatta caaattcgga tttagcgcga aagggcatgc ttcgcaatgg tgactcccat
     1081 ttgaaaaaca ttgccttaat tcaagaagac atcccggcct taacggcgtt catacgatcc
     1141 ttaaacgaag actacgagta aacgacgaac atatcgcctc aagcaaagcc ttccacacgg
     1201 aactctaccc actggaagaa actgaactgc cttaagccca cggtgggtta ttcaccgtgg
     1261 gcttgttttt tgactacttc atttttcagg aagcttctat cggtcccacc actcggacag
     1321 ccaagaacaa aaaagcacct aaaaccatac atcggcaaga actttctttg taaaagatcg
     1381 tcctggttct ttgcacttcc caatccgatt cagttctatt ttcaccactt tagtgccctg
     1441 gttttcacta aatggcccta atatccttca ccttgaggcc gattacaaga cgctcaggga
     1501 ggttgtgccc attaggggga acaggctcta aagcatgggt tggcgcggtg ttggcaatat
     1561 taataatcaa gtgaccgccc attcccggga gaagaagagc ccggaagata gcccctcttc
     1621 gtaagaaaag tgtttattgt ttccgtcaga gtgcatacat atcgacacgg tacaaatcca
     1681 aaccaaaata tcactcccac cgaactcatg gtctgttgcg ggctaacgga ttcgatgttt
     1741 ctttctgaac cctctagatg aactgaaaga ggcatgcccc attcaaaccc taggttcggc
     1801 aggaaaggcc tggaagcaat tgggggacga aatccggcta gggacttcct tgtggtgtat
     1861 ctttcttgat caagctgccc atccggcggg tacgatggaa tatgttcccg gccattggtt
     1921 attaccccgg gttgggtcgc cttttttctc tcgtggactt tcaaggagga tggccaaggt
     1981 gctcccggta caattgcatg attttcattc ggtcctaagt ggttcacatt gtggccagcg
     2041 gtttcgcaca ggaggcttgg gtatcttgaa aagtcattca ttccgtcctt gcacattgca
     2101 aatcgctcat acctgacacc aggatcaatg ggccataccg atttccacta ttttaaaatg
     2161 ccaccagcct cataaaatcc cctcgtccat ccgtccgggc aaaccatctg gccaaaaatc
     2221 ctgcagcaac agatgaagaa cccctattgc ccgtttatca acgcaaaaag ccatataatt
     2281 tccgtgattc ggcatcctga gcctctcggt caggcggact tggaatcctc cagacggaaa
     2341 aaccttctga ctaaaatgga atcgtttcat ttagtttata gattcaaaac atggcttttc
     2401 tgccttttga gcatagccaa aacatttatt ttaatttttt attgaaaata taaatgaaat
     2461 tcattatcat tataaatgaa gtcacaatta caacaatctt tttccattcg gcaaaatcca
     2521 gcccagacaa aatgccaatg aacgcaaaca atcatacatg cgaaaacaca attccagggc
     2581 gagcgagcta cccagtacgc gtccaaaaag gttgggtctc ttttcctcct ccggagcgcc
     2641 caacccttct ttttagtcgg aggacacaaa caatggaggc tatccccatg aacataggac
     2701 attccacact gccacacatg agagtttctg cactgctgaa gggaaatatg atcaatttgg
     2761 atctagcgag cctggaggga caatggggaa tattgtgttg ccttcccaaa cttgaatttt
     2821 gcgatgcagt gtttctcaat cagtttcgta gagccgtcca caaacagggc gccctcctgt
     2881 tgggtatgca acaccctgct cacccggttc tcgtttcgca cctcccaaca attaaaatcc
     2941 tttcaatccc tttattgact gactccgcac aatgtttaag tcaagccttg ggcttatgga
     3001 gtaaacctcc cttaaaccaa tgccagacct ttatctttga cccagaggga gtaatcagag
     3061 accgacggag ccatttgttc aactggcatt gcatgaccct ccttttggac atgttgaaac
     3121 aatgtcagga cgtttcgttt caggcacatc aaaaacatat cgataattcc gttactgcca
     3181 atttcatatt tgcccaacgg ggtctgaaag caaccctctg ctgatactca ctcacaaatc
     3241 aacctcatac cgctcaggag acacttatgc caccaagtca acaactcaag ctgaacgtct
     3301 gtgactgtag tcgttttcat ctcacctatg ggtcaatcac ccttcatttt gagaaggaag
     3361 agttcctcgc ttatgcaatg caattgacca gaattgcggc tcacgtaaca aacaccgctg
     3421 ggtttcgaag ggcacctggc ctccctgaca ctcaaaacac tacctgccat tgaggaaagc
     3481 attcgtcatc catcaactac gttcaaggaa aacatacttg attgcccgta atctggtgaa
     3541 ggaattcaag gaacatctca tgaaacgaga ttcaattatc atcgaagggg aaaagcccgt
     3601 gttcattagt ttgtcggtat ccttggggtt cgtttttctc agcatcattg gcctgtgggc
     3661 atggcccgga atcaggctac tgatagtatt cctggttatt gtttttaccc tattacgtgg
     3721 tcggaaaggt cagtacaaag gatcggggaa attgaacgtc aagccagcat ggataggcaa
     3781 tgggtcttca ttgtaggtat gaccaggatc aaggggtctc ctttgcccaa ccagttcctg
     3841 cgttggttgg tgatcctcat acacaaacag gtcgatggat ggctcaaaag atgcggccca
     3901 ccaatgtgca agtatcacaa catctcaagc cgacagctca gaaacctgct cttgaatttt
     3961 tcccattggc agttccagat aacatcaaaa gccacgctct taaccaaacg atccaaaaaa
     4021 gaagaggaag ttttttatgc aatcagggtc ttcaccagcc aatgagtaaa tcattgcgtt
     4081 gagagaaacc gggagccttc ccccggccca ccccaagatt caaatgaata tccgcaaaat
     4141 tgacaattct ttttcctttt agctatcata aatgataatg aaaattggta tcaaaatcat
     4201 aatagtaagc aagggtaatt tgggagtact tcaatccaaa tgctcacaaa tatccggaaa
     4261 caaccttgcc agtgtctgct ttgctcagac ccagaaggtt tttcactgaa taccaaggag
     4321 ggaacagcat gaagatcgga acgcgtgcac ccatatggaa aacatcatgc ttgctaaatg
     4381 gcagatggca gtatcttccc ctgacaacgt ttcagggaac ccaatttgtg ctctgctgct
     4441 tacctgcctt tactgaaacc gatgcatggt tgctggaaca acaggtcaac cggtttcatg
     4501 caggaggaat cgtactcgca gccttagtac cggacaacgc ctttcttggg aattcctggt
     4561 gtcgtcctcc tcagaacttt ggtcttccct ttttaactga tcctctcaat cgtttgggac
     4621 gtaccctaca cctctcagct accttaccgc cccatcgatc cgaaacatta ttttttgacc
     4681 atcgctgctg cctacaattt cgcctattcc atgacctgaa cctccgaggt atcaccacag
     4741 tgctggaggt tgcagaaagt gatttctgtc ggagatctgc ccaagctgac cttaagtcga
     4801 gactttcttt cgaccatctt ctgccacact gttcatcatt tgaggctcca acaggctgaa
     4861 actcctccgc caacaatgga attctgtacc aacccttgaa ggagaaactc taagatgatt
     4921 gcgccagcct ctgcgttatc ccccgtttca aagaccccat ttctttccat tcaccaatgg
     4981 ctgtatctct tcttgtgcct aatggtgggg atgtatgctg gtcttggcca tgctccctct
     5041 cagtggaccg atgcccttcc agacattatt ctctggggag ccacaggctt ggcagtcggc
     5101 tgcctggctc tatggttaga acgacatttg acggcatgtc accgctctac cttgattggt
     5161 ggtagccttg gcctcatcgt tgccttggcg gggatcggtt ttgtattgaa catgggaatg
     5221 gggacggggc tgctccagat gtcttctctt accccgtgga tcgggctgcc tatctttctt
     5281 ctgtgtccct acatcgggct gatgatcggc attcacatct ccaattcact gtcgccgacc
     5341 tcaatcgcgg aattaccaaa agagtctcct cctcccacct cgcaaccccc atcgaaaacc
     5401 gttcaaaaat tgctggattc tagcgccatt atcgatggcc gaatattgcc actctgcact
     5461 acgggcttct tggaaggtcc tttcatcgtc cccaaaagta ttttgcatga actccagaca
     5521 ttagccgact cggcgcacct ctctaaacga accaagggaa agcgtggctt ggatatctta
     5581 tcccaactgc agcaactgcc aaatatggac gtggtgataa tcgaggattg ggaacccgat
     5641 atatcagcgg ttgaccatca actcatcgcc atagccaaaa accgtgaggc caaaattgta
     5701 accaatgatt ggaacctggc aaaagtcgca gcgcttcaag gtgtgccctc cctcaatgtg
     5761 aacgaattaa cctaccaatt acgccccttg gtattgcctg gagaaaccat acgtgtcttc
     5821 attcacaaag aggggcaggg gcaggagcag gggattgccc atctagatga tggcacaatg
     5881 gtcgtcgtcg accacggtgc gactctggtt ggtcaagcga ttgaggtggt ggtgacccgg
     5941 ttcatgcaaa ccaacacggg aaggatgatt ttctcaactc cccagtccaa acactctcca
     6001 ttacctgtca attttcaaac ggctctcaag aattcagagg tggtttcagg atactctcgc
     6061 agcctcgtga aagatcatgg atgaggatgg aaagatagca atactaccca aacatgtttt
     6121 tatgatacga cattaatatt tatttacctg gaggtctcca ctaaccgaaa tgtcagtcta
     6181 tcccggagag ggctcttccc ctcttcaagc tagtgggggg cccatccgca ctcctgggtc
     6241 ccctactttc tttgatgttg gcttttgaca ccaatcacct taagaaagga atcaaccatg
     6301 tcattccaac gctgtcctca atgccagaac agcacgcttg caccaaccgg agcattttgg
     6361 tgctgtacga attgcggatc tgcaattact tcccaggccc tagccgccct aagggaatca
     6421 gtcaaaacca gaggccatcg gcaggcccac ctcatccatg agatcctcta atgctctatt
     6481 gacgaaaata ggttattctt cagagagggt gagttcatcg caaccactgt gtcacggccg
     6541 acccctggca ccatacggaa tgggccatta cctagacgtg accagtctca tttttcatgt
     6601 cgccacgaaa gggctcgtgg cataaaatca gatcaggatc aaagccaagg ttccaccttc
     6661 tgatctcttg gatgagaagc attcttttcc cagaatgacc tatctgtttt gctggtcacg
     6721 gcgagtgatt tcctcacaca ctattccccc gtaaagcaat actttctaca ttgatcctcc
     6781 tccattgact gcacaactca actgttttgg gctaaatcag atgcccaaac caggtgaatt
     6841 tgaggaggat gtcaggcagt gccgccaatc attaatatcc aaccctgacg atcggggaat
     6901 ttccggaacc ctctcccgtg tatttaaact ttctgaagat gccgtgggac aatgcttgat
     6961 ggccaaaggc tggtttttgg ctgaaaaacc ctaatctatg ccaaatgccc ttttcgataa
     7021 ttcaccagcc cggcaaatgg tgcatatacg caagaacgac cgaaattatt catccagaaa
     7081 ttgatcaacc gcggcatgaa gagcatgttg aatttgctga cagtcttccc cggaaagaat
     7141 tttttctccc tgagaattgg tcacataaaa aacatcggcc acctgatcta acctggtccc
     7201 tattctggcc atatgaattg acagacccaa ttgaaccagc gttttggcaa ttacaaagag
     7261 cagcccttgc ttgtcatcgg caaagacgtc aatcaccgta aaggcatctg agaccccatg
     7321 atcaatatgg acttccgtgg gatgccggcc agtaggaaag ggacgaccaa aggccatgcg
     7381 ccgtcgtcgt tcaaccaatt ggtgaactga ttgtcttccc tccacgacct cttgaatttc
     7441 ctttgccacc tcctctaggc gcccggcaga tggcggccct tcatagtctg aatcatgtac
     7501 cgagaacaca tcccacaccg tgccatcctt cagggtcata atttgcgctt ccaatacctg
     7561 caaacccatg gcggctaaca ctccggccac ttgcatgaaa atacctggtt tggtctgttc
     7621 acgggtgatc agcacatact ccgaaacacc tgaggcctga ttgaatctgg cttccacatg
     7681 agtcggcttg actggtagag aacgaatggc taaaagatac gccgccattt ggtcaaccgg
     7741 tgtcgactga acgtaccggc tgggaagttg ttgaagttgg gcattcaccc attccgacca
     7801 gtcgggttca ggagaatggc cctcgcttcg atcggccctt aaaatcttga taagtttgtc
     7861 tcgagcttcg ggcggaagat tcttactccc atctccggaa tacgatggac ctgcatcccc
     7921 ggcaagctct gcgtaggtca gggaatacaa ttcccctaat aaggattctt tccatttcgt
     7981 catcacctca ggtcccaccg ctgatatatc tgcaatagtc agaataaaca gcttttgaag
     8041 catcccgaca gtttttacca atcgggcaaa tgtgacaatg gttttaggat cgttgagatc
     8101 tcggcgaaaa gccgtatgag acatctgcaa gtgatggcga accaggaaag cgagagtctg
     8161 ccgttccttc tcagataaat ccaaccgatc agctgttaaa tgggcaatct cctgaccgac
     8221 ttcactatga tccccaggac gccctttgcc taaatcatgc agcaacagcg ccagatgcaa
     8281 aatgtcttta tcagcgatgt ttcggtaaac ctctcctatg atcccttgat gattgtgaag
     8341 ccgttccgct tccctcactg cgagcagact atgctcatcg accgtgtatt tatgatattg
     8401 attgaactgc atgaggccac gtactcggga gaaagccggc acgagctttt ccagtagact
     8461 agcctgatgc atggcctcaa gaatttgagc tatccgacca gggcttgaca gaatgtgtcg
     8521 aaaaagccta ctcaccggct tggtatgaaa ccgctcattg ggtaaggttc ccatgtgctg
     8581 agacaattca ttgagaatgt gcgaatcgat ggggactgct tgacgttgag aggtcacaaa
     8641 taaactcagg atcagcatgg ggctctccat cacctcaagg agtttatccg actgaatggt
     8701 cagccgattt tccataatac ggaagtgccc ctcaattaat gggggaggcc accattggcg
     8761 aacccggtcc aaccaggatt cctccctggc ttgatcaaga aaccgccgcc cacggtcaaa
     8821 caacccggtc gtatgacgga aatacacttg cataaattgc tcaacggcca acaaatgaga
     8881 atgatccaca aatccccaaa gcgcagagag ccgaacctga tcgtcgaacg taaggatatc
     8941 ttgagcccgt cccgcttcaa aatgcaaaaa acaccgaact cgccaaagaa attcctgcgc
     9001 ttcctgaaga acctgataat cctgaatggc aattattccc cgattcgaga gttcttgaat
     9061 cgtccccgct ccatatcgag cctggccaac ccattgcagt aaatgcagat cccgaagccc
     9121 ccccttactt tttttaatat tcggttccag cataaagacc gtttcaccaa atttggcata
     9181 ctctcgctct cgctcttgaa ttttatccag aacaaaccgt tgcgcccgtt tcttgatgat
     9241 ccgacgttcg aattttcgtt gaaattgctg aaacaccgaa gcactgccga taagaaatcg
     9301 tgattccatc agtgaagtac aggcagccag atcggcctct gcaatcgtca gacattctac
     9361 gagggaacgc acactatgcc caacttgaaa cccaaggtcc caaagccggt gaaagactcc
     9421 ggtagacagt gcctcagcga cttcccgttg attgccctga gtgagtatca tcacatcgat
     9481 gtcggaatat ggagctaact cccgccgccc ataccccccc atcgccacta aacaacattg
     9541 ctgccatcca gctcgcacac ctgcattgcc ttgctgaatg acctcccgaa accgcgcgat
     9601 cagtaaagca tccatgaaat ccgaaaaagc ctgggtgatc tcactgccgg tcgccccttc
     9661 aattaagcgt tgccgaagaa cctcgcgctg ggactggaga acatgaagcc ccagagagga
     9721 gtccttgtgt tccgtcaaac cggtaggggg caacgacatc ccttcactca tcgagggcaa
     9781 ctcccgcaac actaaacatg tcacacattc agattaagct ccccaaaaat atcggttcgt
     9841 aatcggcatg cgccgatcct ttcctaacgc tctcgaggta attttaatgc ccactggcgc
     9901 ttgtcgacgc ttgtattcgc tccgatccac catccccatg acctttcgta caacatgagt
     9961 atcatagccc atcttcacaa tcctgcccat tgactcattg tcctcaacat aggcttgtag
    10021 aattgcatct aacaccggat agggagggag ggtatcctga tccgtctgat cgggcctcaa
    10081 ctcagctgaa ggagcacgca cctgaattct ttcaggaata atcacttgat caaaattaga
    10141 gcccttatac tcacttcgcc agcgagaaag ctcatacacc ttcgttttgg gaatatcttt
    10201 gatcacggca aacccaccag ccatatcgcc gtacagcgtg gcatatccca cactcatttc
    10261 gcttttattt cccgtggtca gtactaaata cccgaactta ttggaaagag ccatcagcag
    10321 tgttcctcgg atgcgagctt gcagattttc ttccgtcgta tcagcctgaa aaccctgaaa
    10381 ggacttgccc aacacccgaa gcaaaacctt gaacgccctc gttatcgaaa ggtgccgcat
    10441 ctcgattccc aatttttttc ctagctgacg ggcatcctct cgactttccc tagaggtata
    10501 gggggatggc atcattacac ccatcacatt ggacgcacct aaggcatctc gggcaatcaa
    10561 tgcagtcaaa gccgaatcaa tccctccgct gataccgaca agaacccttg agaaggcatt
    10621 cttcctcaca taatcttgca ctcctagcac gagtgcccgg tatatctcct ctgactcacc
    10681 gattaccggt gtgagccttc tgacacctgg aatgccagac ttcgaccaat ttggcgccca
    10741 ccccattcga atcatcggaa cccgaacatc ttcgccatca actttagata ctttttcagt
    10801 ccccttcgat ttcctctttc gtgttgaagg aatctgaagg tcggtcagca ggaaatcttc
    10861 ttcaaaggct tttgcccgcg caaggaccac accggtggaa tccatgacga gactattccc
    10921 atcaaagacc aattcatcct gtcctccgac catattggta taactgacca ttacattgtt
    10981 agctttcgcc cgaatggcta acatgcgttc acgattttgg gtttttccga cgtgataagg
    11041 ggaggcatta atgttgagaa ccaggtgagc gcccccatat gctgcctgcc ggctggccgg
    11101 accatcagca aaccatatgt cttcacaaat attgattcca attcgtaacg ggccaagaca
    11161 gtagaccgga ctagttcgtc ccggttgaaa ataccgttct tcatcaaaca ccccatagtt
    11221 ggggagcttg catttggcat acgatctttt cacctgacca ttgaccagga cccacgctgc
    11281 attcaaaggc ttccttcgat cctgcccgtg atcccggagg ggatgttgag caagacgatc
    11341 agattcaatc acgcttccca ctaccgccat aatccccgtc gtgactttgg ttatccggtc
    11401 ccgcatccga ctaatatcca atagaaactt cggcatgagg agcagatctt ctggaggata
    11461 cccacacacg gccaattccg gaaagactac cacatcggct ttggctttcc tcgcctcgcg
    11521 aatccatccg caaatggtgc gggtattccc ctcaaggtcc ccaaccaccg cattcatttg
    11581 caccatcgct aatcgaatca tcatgataaa aaaaacgccc tctgtcccat cggaaccgag
    11641 gacgccattg tcctttgcgc taacatatac gggaagacgt cgttgtcgcc cctcaaagta
    11701 ttatacaagc aatgaagaat ccgttcaaag aatctctcct caaattccta tttcaataat
    11761 aaatcgccct agggatgctt attgagtcgc ccgttgaagt tcctctgcga gaataatcgc
    11821 atcggcaatt tctctcatgg attttcgaag attcatgctt tgtcgttgca taagacgaaa
    11881 ggcttcaggc tctgacaatc ccctagacgc cattaaaaac cctttagcac gctctaccaa
    11941 ttttcgcact tctagagcct cttgcatctc aaacgacttt tccataagcc gagtgtgctc
    12001 aatggcgacg gcagattgat tcgcaatggc ttgaagcgtg gtcacctctt cgtctgaaaa
    12061 cgcatgatag ctggaagtgt atacattaat aaccccaatc ggattttcct tcatgagcat
    12121 gggaatggac aataaagaat gcaatccttc ctgtttcgcc aaatcccgat aaaaataccc
    12181 atcttctgcc ttcacatccg agacatacac cggatgttgg tcctgcaccg cccggccact
    12241 catactctgc ccaaccttca acggtggctt ccgccggtac gcatcactca ggctttgagt
    12301 ggctgcaata cgaagttccc ccgaagcctg gtcgacgagc atgatcgagc aaatttttga
    12361 attcatcagt tgagccgtca tggtgacaat taattgcagc acatcctcta tcagtcgatt
    12421 agaagccacc gtttccgata cttgggaaag ggtttcaagt cgacgcgctt tttgccgcat
    12481 atcatcgtat aatctggcat tggcaatggc gccgccgact tgattagcaa tggtcagtaa
    12541 gagcgctagc gtttcttcag cataccgctt tgaccgctta tgctgaacat tgatgacgcc
    12601 gaccattttc cccttactcg taatgggaac tgacacaaac gcctgatacc tgtcttccgg
    12661 cagacgatga aaaaacttaa atctggcgtc atcacttgca ttgcgaggaa tcaccacggg
    12721 aattttttct cgtgccaccc acccggtaat tccttctccc aaaccgatgg atatccgccc
    12781 gataagacgg ggatgcggat ttttcgatgc acgcaaaata agctcgtctg ttgtttcaga
    12841 gatcaagtac aacagacagg catccccctt cgtcacttcc acaaccactt ccacgatttg
    12901 ctttagtacc gtttccagct ccaaagtact actaatggat tcgctgattc ggtgaagaat
    12961 ctgcacctca cgcgtccgct cccgaacaag gtgcttgagg tcttctatcg tcgaaggacg
    13021 gatcgtagcc atgttatttt ttcttggaga gacggctcac tgcctgtgga acaggatcca
    13081 atcgttttcc acgaaaggac tgactggcag taaaccattg gcgaatacgt tgtcgaacca
    13141 ggggaacgac cttgaccttt ccgattgtgg tgggtaacac gcaattaatt tgacccttta
    13201 cgaccttttt gtcgtgttgc atggcccccc agagttccat aaacgtcatg gaaggcatga
    13261 caatgggcaa cccaacatcc tgaatcaagt cgcgttgccg ctcaaccagc tccttcgtac
    13321 acaaccctaa aaaatgtgcc attgcggctt cctgcaccat gccaattccc acc
//
LOCUS       DATIAL010000497         2259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_627769, whole genome shotgun sequence.
ACCESSION   DATIAL010000497 DATIAL010000000
VERSION     DATIAL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627769"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VLA60_15310"
     CDS             <1..493
                     /locus_tag="VLA60_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HSF10778.1"
                     /translation="QLELDSRSMKQLTSHNSADLSPSWSPDGRHLAFTSDRGGRPQIY
                     IMDADGSREPRRLTYDGDYNAAPAWSPKGDWIVYVCRMPNEGFKLCRISPDGEKRKQI
                     TTGQSIDDSPSWAPNGRHIVFSSIRRGESQLYIITSEGAGLEKISSTGEHLSSPSWSP
                     FQP"
     gene            541..1497
                     /locus_tag="VLA60_15315"
     CDS             541..1497
                     /locus_tag="VLA60_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HSF10779.1"
                     /translation="MRPVIQVISLFGILGLSLMSLTGCGEKRIHVATVSGAPGEEMNG
                     ASTTNPSAGISGNSVDESSLTGQGSTPQAMSAESIQPVAPVEPLNFAHEPSSPAPTDD
                     SFMRDDISRPSTDAPADMNAQTHSQIADNSQLSTGSPSDFSTQSGSSSMNGSPHDQTE
                     GTAFPAPAAFQGDMEPIPDSFQVAKAEPSDMLRQQLDKIKEEELATASAGLEDVFFQF
                     DSWSLTAEAKESLERDLGWLTSDPSSLLVIEGHADQRGTQAYNMVLGKKRAMAIRDYL
                     AQSGVDPSRLSVISYGKDKPFCQDATEVCYQLNRRGHLLVQN"
     gene            1549..>2259
                     /locus_tag="VLA60_15320"
     CDS             1549..>2259
                     /locus_tag="VLA60_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10780.1"
                     /translation="MKTGLSLRASSRQRALLSSLKWVLACGICSVLVTGCVAQQADVA
                     RIQKDLENQIAKIKEEKKSLGLQVDETKAQLIKMNEEAKKTRGNLASINQKATLLQEK
                     DVASLYGKLEETEKSIKDLRKDFTNQTDLLKSEVQNQGEELQSAKTHNTTIDQKVDTL
                     AQQVDENNQALTVNMTDFQGSLAQFKDTLTSLGATIGTVKTDLTAQQQELGTVKSRTE
                     ELAQSTHQIRETLGKSGTE"
BASE COUNT          648 a          578 c          540 g          493 t
ORIGIN      
        1 tcaactggaa ttggacagta gaagcatgaa gcagttaacc tcccataata gtgcagattt
       61 gtccccgtca tggtctccag acgggcggca tctggccttc acctcggatc ggggaggccg
      121 cccccaaatt tacattatgg atgccgatgg gtcgagggaa ccccgtcgat tgacctacga
      181 tggcgattat aacgccgctc cggcgtggtc tccgaaaggc gattggattg tgtatgtgtg
      241 ccggatgcct aatgagggat ttaaattatg ccgcatcagc ccggatggcg aaaaacggaa
      301 gcaaattaca accggacaaa gcattgacga ttctccctca tgggctccga atggccgaca
      361 tattgtgttc agttcgattc gaagaggaga aagtcaattg tacatcatca ccagcgaagg
      421 agccggcttg gagaaaattt cctcgacggg ggaacatttg agttctcctt cgtggtcacc
      481 atttcaacca taatccacat gaacccccaa ccgtaaatat tccaaaaaag gagtaccctt
      541 atgcgtccag tcattcaagt aatttcactc tttggcattc ttggtctttc gctcatgagt
      601 ttaacgggat gtggagaaaa acgtatccac gttgctactg tttccggtgc ccccggcgaa
      661 gaaatgaatg gggcatcaac cacaaatccg tccgcgggca tatcaggaaa ttctgttgat
      721 gaatcttccc ttacagggca aggttcgacg ccgcaggcaa tgagcgcgga atccattcaa
      781 ccggttgcac ccgttgaacc actcaatttt gcccacgaac cgtcaagtcc cgctcctacg
      841 gacgactctt tcatgaggga cgacatttca cgaccatcga cagatgctcc cgctgacatg
      901 aacgcgcaga cacactcaca aattgcggac aactcccagt tatcgacggg atccccttcg
      961 gatttttcca cccaatccgg ttcttcctca atgaatggtt caccacacga tcagaccgag
     1021 ggaaccgcct tcccggcacc ggcggcattt caaggcgaca tggaacccat tccagatagt
     1081 tttcaagtgg caaaagcgga accctccgat atgctgcgac aacagttgga taagattaaa
     1141 gaggaagaac tggctaccgc atccgccggg ttagaggatg tattcttcca atttgatagc
     1201 tggtctctca ccgcggaagc caaggaatcc cttgaacgtg atctggggtg gctcacatcc
     1261 gatccctctt ccctgttagt catcgaagga catgccgacc aacgtggaac ccaggcctac
     1321 aacatggttc tcggcaaaaa acgggcgatg gccatccgag attatctagc ccaatcaggc
     1381 gttgatcctt cacggttgtc agtcatttct tatggaaaag acaaaccgtt ctgtcaggac
     1441 gctacggaag tttgctatca actgaatcgt cgaggacatt tgctcgttca gaattagcag
     1501 gctttctcat cgatcaatga ctgttcagca tggagggata attacagagt gaaaacaggt
     1561 ttgagccttc gagcttcttc aagacagcgg gcattgcttt cctccctcaa atgggtattg
     1621 gcctgtggca tctgcagcgt gctggttacc ggctgtgtgg cgcaacaagc cgacgttgcc
     1681 aggattcaaa aggacttaga aaatcaaatc gccaaaatca aggaggaaaa aaagtctctg
     1741 gggttacaag ttgacgagac caaggcacag ctcatcaaga tgaatgagga agccaaaaaa
     1801 actcgcggca atctggcgtc tatcaaccag aaagccacgc tgttgcaaga gaaggatgtc
     1861 gcgagtctgt acggaaaact tgaagaaact gaaaaaagta tcaaagacct gagaaaggac
     1921 tttaccaacc aaacggatct tttgaaatct gaagtccaga atcagggaga agaactacaa
     1981 tccgcaaaaa ctcacaatac aaccattgac caaaaggtgg ataccctcgc ccaacaggtg
     2041 gatgaaaaca atcaagccct cacagtgaac atgactgatt ttcagggttc cctcgcacaa
     2101 tttaaggata ccctgaccag cctgggagcc acaatcggaa cggtcaaaac cgatctaacg
     2161 gctcaacagc aagaactcgg aaccgtcaaa tcccgtacag aagagttagc tcaatccacg
     2221 catcaaattc gggaaacatt aggaaaatcg ggaacagag
//
LOCUS       DATIAL010000498         3136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1272646, whole genome shotgun sequence.
ACCESSION   DATIAL010000498 DATIAL010000000
VERSION     DATIAL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272646"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..432
                     /locus_tag="VLA60_15325"
     CDS             181..432
                     /locus_tag="VLA60_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10781.1"
                     /translation="MPFLESLSQKFSPDQLAILAVTTDIRPREIEAFWQELGLHFDVP
                     LDEQRELSQALMVRNRATPTIVDIHRQPEPMNPMTQGVE"
     gene            998..1729
                     /locus_tag="VLA60_15330"
     CDS             998..1729
                     /locus_tag="VLA60_15330"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HSF10782.1"
                     /translation="MKQFLSVCMWNWSWRFFMVILSLPQILSVSSPTFAASSDTSGVS
                     QESLLQQQQELEGELDHVNARLNEIRKQLRELHQTPVSHQSDEQRQTQATLEEEEVET
                     LEEMSIVSTRILKPPEGLTMSSTPQSETDSQPTRTVKESMESLPGVVLRQANGPRDFS
                     ISIRVSGIKTSFLIRDIKVYEDGFQQTQSDGLSRLDIQDPWFMRSTEVIRGAASSLYD
                     NYALGGMVHFRTRRGQRHPGSREVL"
     gene            complement(2239..2739)
                     /gene="smbP"
                     /locus_tag="VLA60_15335"
     CDS             complement(2239..2739)
                     /gene="smbP"
                     /locus_tag="VLA60_15335"
                     /inference="COORDINATES: protein motif:HMM:NF028095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small metal-binding protein SmbP"
                     /protein_id="HSF10783.1"
                     /translation="MISKVSTLIRIVGCVGVLGFLAVPAVTLAEADKHLHEAIGHAGE
                     AKAHGEMGHAGEAAKHAEEALLHADAAKQEGANAHVGEGISHLGEAVDHSKQGHGEVA
                     GEHSQEALRIPARRRGSNSVRRSALLENVPRYFEWFPGYSGSMSLAYPGRRLNFGSFD
                     PPVPCY"
BASE COUNT          796 a          881 c          675 g          784 t
ORIGIN      
        1 gtggctttgg gaaatccttc atccaccccg atccttcttg gccaattcta taccggtcaa
       61 ttcctcccgc ctttccggct cagcaccttg cagggcgcaa cagttcattc gaacaaattg
      121 gaaggacaag ttctcatctt gaatttttgg gccacctggt gttgacttta taaagaagaa
      181 atgccgtttt tggaaagtct tagtcaaaaa ttctctccag atcaattagc cattctggcg
      241 gtcaccaccg acattcgtcc acgagaaatc gaagcatttt ggcaggaact cggtttacat
      301 ttcgatgtcc ccctggatga acagagagaa ctttctcaag cgctcatggt cagaaatcgg
      361 gccacaccca cgatcgtgga tattcacagg caacctgaac caatgaatcc tatgactcag
      421 ggagtggaat agctctgaat ccgtctcctt catcaaaatc ctcttcaacc cgaagtcaat
      481 gcgccaacgc cttcaaccga ttgccctcct gacctcaagc ctgtttctcg ccgtgcttct
      541 ggcttccacg gtgtgcatga ccgtgcatga cctctctctc gattctccgg aatcccatca
      601 cagcaatacg aaacctcttc atggggttct ttgcaactgg gcctgtcata tcggaaaaat
      661 ttcgtcaacg tccctcccca cctctccatc gtggcattcg ccattccacg aagtgggcgc
      721 cgtactttct ctctataccc tgcctggctc gcttccttct ctttcgaacg aatctgccag
      781 agctcccccc cacgttctct tgtcctgttt ttcctaaatg attctcaagg ctccattgtg
      841 agttgaggga gattcacgcc tccttccaca caattatagc tcgagcctgg gctcatccga
      901 taattctttc aggaagcctg attcttagtt gctggccaca tgcatagccg gttggctcat
      961 gcgtgttcta tctatttttt tgtaaggaga gtatctcgtg aaacagtttc tttctgtctg
     1021 tatgtggaat tggtcatggc ggtttttcat ggtcatcttg agccttcccc aaattcttag
     1081 cgtctcatct ccaacgttcg ctgcctcaag cgacacttct ggtgtgagcc aagaatccct
     1141 tcttcagcaa caacaggagt tggagggaga actcgaccac gtcaatgccc gactcaatga
     1201 aatcaggaaa caactgaggg aattacatca gacacctgtc tcccaccaat cagatgaaca
     1261 acggcaaacc caggcgacct tggaagagga ggaagtggag accctagaag agatgagtat
     1321 cgtcagtacc cggattttaa aaccgccgga agggctgacg atgtcctcca cccctcaatc
     1381 ggaaaccgat tcccaaccca cccgaaccgt gaaagaatcc atggaatccc ttcccggtgt
     1441 ggtgctccga caggccaacg gccccaggga cttcagtatt tccattcgag tttccggaat
     1501 caaaacgtcc ttcctcatcc gggatatcaa agtctatgaa gatggatttc agcagacgca
     1561 gtcggacgga ttgtcccgac tcgacataca agacccgtgg ttcatgcgat cgaccgaagt
     1621 gatccgtgga gcggcttcct cactgtatga caattatgct ctcggcggaa tggtgcattt
     1681 tcgaacccgg cgggggcagc gacatccagg gagtcgagag gttctttagc ggagtatcat
     1741 acgggtataa caagcaagcg gtcgccctcg gcacccaata caaaaatctg gccgtgtcga
     1801 tatttgccag taacgcggct gagaacgggt ttatccatca cagtggaaca cccagacgtt
     1861 gaattttaat ttccgcttca ccatcgacga catacaaaac ttatatataa aagccatgac
     1921 caactggctg gatacgaacg tgccgacccg gctcacctaa aagccaattt aataaaaatg
     1981 atcgccaggc gggaactgga acggacgcca gagatcaagc tcgcctcgac cgccgcacca
     2041 tcatcgggtt gatgtatgaa cgggaattga ctcccgacct catcttgacc atggaagggg
     2101 attatgacgt caaggatatt aaccaatatt tcaaccaaat caccgataac gtaaatacca
     2161 attgaaaaca ttatactgat ttgcgacaca ggggccagat ctccggaatg cccttgaaca
     2221 gctatctcgg gttttttgtc aataacatgg aacaggaggg tcaaagcttc caaaatttaa
     2281 ccgacggcca gggtacgcga ggctcattga tccagaataa ccggggaacc attcgaaata
     2341 tcgggggacg ttttcgagaa gagctgacct tcttaccgaa ttggaccctc gccgccgggc
     2401 tgggattcgc aaagcttcct gactatgctc gcccgcgacc tcgccgtgtc cctgtttgct
     2461 atggtcgacc gcttcgccga gatgactaat gccctcacca acatgggcat tggcaccttc
     2521 ttgtttggca gcatcggcat gcaaaagggc ctcctctgca tgcttcgccg cttcaccggc
     2581 atgtcccatt tctccatgag ctttggcctc accggcatgt ccaatcgctt cgtgcaaatg
     2641 cttatcggcc tccgccaatg tgacagccgg aaccgccaaa aagcccaata ctccaacaca
     2701 accgacaatt cgaatgagtg tagagacttt actgatcatg tcttccctcc attgattaat
     2761 aagtcaccac aaatcacaag cctattacct cacggaacgc ccgctccatc ttctaacgcc
     2821 atgttggtca taatcatcta tgccacttac ctcaacccac atgtatgact ctttacccga
     2881 cacacaatgt caactacaaa ctaattttaa ttttgtgcca caaagacccc ttattcataa
     2941 ctgcgtcgct ccggcgtatg tatcattgac cacacataag attttctgac tcttcataga
     3001 atgcaatgga taatgtgttg catgcatggg caaccgtctg tagacgattt ttatggctag
     3061 tcctatactc tgcccaagtc agacagctct ttatgagctt ctgttacgat ccgacccaaa
     3121 agaaaatcgg gacgaa
//
LOCUS       DATIAL010000499         7517 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_842529, whole genome shotgun sequence.
ACCESSION   DATIAL010000499 DATIAL010000000
VERSION     DATIAL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842529"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VLA60_15340"
     CDS             complement(<1..1281)
                     /locus_tag="VLA60_15340"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HSF10784.1"
                     /translation="MTALSSSITLSLISHTNIGKTTLARTLLRRDVGQVLDQAHVTLQ
                     NEQFVLLETSDGSRLNLWDTPGFGNSHKLLVRLQGLNNPIGWMVSQVWDRIADKPFWC
                     SQQAIRNVRDEADVVLYLVNATEDPSMAGYLQPELDLLTWLNKPVIVLVNQTGLIDPQ
                     QQRQLESLWKQHWVNQRVIKDVMSLDAFTRCWVQEGVLWDHVTQVLPSGKHPTMEKLG
                     KAWYAIHRQIFDTSMTHLTQLLIETAMDGECLPQEPTGFSKKLQIKKAIQALDQRLAQ
                     RISGVTADLIELHGLTGDAAHTLKSRIEDVTVPGEKKPWEEETFWGALASGAAAGLAS
                     DLATGGLSHGAFTIGGAILGAFAERTYAKSQETGDSNRISWVPAFLDRQTRDALLRYL
                     AVTHCGRGRGDYTDPREFPVFWQRAAENVLEQQKD"
     gene            complement(1288..2769)
                     /locus_tag="VLA60_15345"
     CDS             complement(1288..2769)
                     /locus_tag="VLA60_15345"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HSF10785.1"
                     /translation="MNSKQVTKILLIQALEQSDPQGRYISHSTRQRASQQAKKSSPQE
                     PPSSAESSIQFFTNRAESLWNFLSTSYPMITESFRGAQATIPYTIVAIPAFAVGLFIN
                     GLGTTQRVNLLNFPLLILLLWNMGTYAGTILPPLLGKDLTGPLLRHLAKGFATVAEWL
                     GKGPWPKIALPGGAVKEWVLQSSERFMNLWWRHWHPVIISRVRHMLHIGSACLALGII
                     LSMYVRGLVLDYQATWESTFLSAAQVHTVLYGLLGPAAWLLGFPFPSAEDLAHLQAPG
                     QGSAAQWIHMWALTAFVSIVIPRVTLAWLSARFAHKAAQSFTLPLDEPYYLQLLSTER
                     GQGIQIDIVPYSYQPSPAALDCLGQCLLDLIGNQATLHWRDPLPYGQTSVTWLQTTAS
                     PQTVVLLCNLAQTPEAEVHGELFHMVQASIESCNGQHHLLLVLDQEPYRHLADQTQMR
                     ERQQTWQRLANDYHLQIVAFDAKETSRDKLLEKAQAALWPPKM"
     gene            complement(2984..4129)
                     /locus_tag="VLA60_15350"
     CDS             complement(2984..4129)
                     /locus_tag="VLA60_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF10786.1"
                     /translation="MHNQQNSQLPPSSHPPTGRFLLTTAAFIIVVAGMRAAEPILVPL
                     IVSIFLAIISASPVFWLRQKGIPSPLAVFGVVLGVLSVGIVFILIIGTSLDDFSEAIP
                     RYQARLTQEIEPMIHWIQELGFQLDKGILLKSIDPAASMRLVAKMLSGLGNVLTNSFL
                     ILLTVIFILFEASSFQEKVGTAFGDPKGTFSQAKKITDAVNNYLAIKTVVSLGTGVIV
                     ALWVWALGIDFPLIWGLLAFLLNFIPNLGSIIAAVPPMLLGYIQFGIGHLLLVAAGYV
                     AVNLIFGNVVEPKLMGRKLNLSTLVVFLSLVFWGWVWGPVGMLLSVPMTMVVKIALES
                     SPSTRWISILLDSEASASQSVNNNSQEPQPDASSSESKHAGSVSLHT"
     gene            complement(4317..5270)
                     /locus_tag="VLA60_15355"
     CDS             complement(4317..5270)
                     /locus_tag="VLA60_15355"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HSF10787.1"
                     /translation="MTDSILRSNDRIEVDGPDINLKRKAFGMRGETMRLSIWVILCLA
                     IGLGGCAGKSKSTKTQDGMETGPDIQIAEFEDGLNAEGEENEFFDPFDESGDSQNKDY
                     DPFEPVNSAVFEFNYRLDKYVVKPVAKVYNFFIPPDVQQSFSNVFQNIRFVPRLLNNI
                     FQAKFQGAGIELSRFLINSTLGVGGLFDPAGIMFELETPQEDLGQTLGTYGVPPGPFL
                     MIPLLGPFTLRDGIGFIGDSFLDPFNWLVLPFIEIADAPRLVQHDMTVTLSRLGMVAG
                     ETVNLRALTLEKFQGVEEGTLDLYGAVRNGYLQQRRKAIQE"
     gene            5767..7389
                     /locus_tag="VLA60_15360"
     CDS             5767..7389
                     /locus_tag="VLA60_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF10788.1"
                     /translation="MSPSVSEQKPSWNGSIKRVKKQDRHPVSSGGGSPEADRSSGVSD
                     KQLPLYLKEIGQVSLLDREGEVRLCKKIEEARRSMLEILYSLPMTLDYLQEQRMRLLN
                     GEILAKHVVQREKEVDVETESEEEPDIPDVQTEQQEDEEFRQRVIQQLSHLCHCVQFL
                     MAKEHVSASKQGASASMQARKKLWQSLEEVNWHPAFTKQVESRIRATERQLTEVLKRL
                     GLSPQDIFNGDERTRGVGHNSLPAINPGVKVNSGSHRSTLEAGEHLVYLEQEVLRLCF
                     SEFLARVRQLDQAKIRLHFAKEAMLEANLRLVVSVAKRYVNRGLDLLDLIQEGNIGLM
                     RAVDRFEYQRGYKFSTYATWWIRQAVTRALADQSRTVRIPVHVCDVLTRVRRTLDRLA
                     VQLGREPKLEELSSAVDIPLDKLSTMLEATKGTLSLETPMGDEDGSPLSDLLQDPTAV
                     SPCYSAERVDLQEKVTYLLGTLTPREAHIIRRRFGIGELEDATLEEIGLEFSVTRERI
                     RQIEERALAKLREPQRNVVLRNFFQPSGPALD"
BASE COUNT         1957 a         1734 c         1970 g         1856 t
ORIGIN      
        1 gtctttttgt tgttcaagaa cgttttcggc ggcgcgttgc caaaaaacgg gaaactctct
       61 tgggtccgtg taatctcccc gccccctccc gcaatgggtc accgctaaat agcgtaacaa
      121 ggcatcccgc gtttgtcggt caagaaaggc agggacccag gaaatccgat tggaatcgcc
      181 tgtttcctga cttttggcat aggtccgctc ggcgaatgct ccaaggatcg ctcctccaat
      241 ggtaaacgcg ccatgagaga gtcctccggt ggctaagtcc gaagccaatc cggctgccgc
      301 gccactggcc agtgcgcccc agaacgtttc ttcttcccac ggtttttttt cacctggcac
      361 ggtcacatct tcaatcctgg atttgagtgt gtgcgcggca tcgccggtta atccatgtaa
      421 ctcaatgaga tctgcggtga cgcctgagat gcgttgtgct aaacgttggt ccagtgcttg
      481 aatcgctttt ttgatttgca gcttttttga gaaccccgtc ggctcttggg gaaggcattc
      541 tccatccatg gcggtctcga tcaagagttg agtgagatgg gtcatggatg tatcaaagat
      601 ctgccggtgg atggcatacc aggcctttcc tagtttttcc atcgtggggt gttttcccga
      661 gggcaagacc tgagtgacgt gatcccataa cacaccttcc tgaacccagc aacgcgtaaa
      721 ggcgtccaag ctcatcacgt ctttaatgac gcgttgatta acccagtgtt gtttccaaag
      781 ggattccaat tgtcgctgtt gttgtgggtc gatcagaccg gtttgattca ctaacacgat
      841 gacgggttta ttcagccaag tgaggagatc gagttcaggt tgaagatagc cggccatgga
      901 tgggtcttct gtcgcattga cgaggtataa caccacgtcg gcttcatccc tgacgtttcg
      961 gatcgcttgc tgactgcacc aaaacggttt gtctgctata cggtcccaga cttgagacac
     1021 catccaccca atggggttgt tgagtccttg aagacggacc aagagcttgt ggctatttcc
     1081 aaaacctggg gtatcccaca agttgagtcg ggagccatca gaagtctcca acaatacaaa
     1141 ttgttcattt tgaagcgtga catgggcctg atcgagcacc tgtccgacgt cgcgacgcaa
     1201 cagagtgcgg gccaaggtgg tcttcccgat attggtatgg gaaatgagtg aaagcgtgat
     1261 agaagaagaa agggccgtca tgtcatctca catttttgga ggccacaaag cagcttgggc
     1321 tttctccaga agcttgtcgc gggatgtctc tttggcatcg aaggccacga tttgtagatg
     1381 gtagtcgttg gcgagtcgtt gccaggtttg ttgacgttct ctcatttgtg tctgatcagc
     1441 aaggtgccga tagggctcct ggtccagaac aaggagtaag tgatgttgtc cattacagga
     1501 ttcaatagaa gcttgaacca tgtgaaagag ttcgccgtgc acctccgctt ccggggtttg
     1561 cgccaaatta cagagaagaa ccaccgtttg aggtgaggcg gttgtctgca accaggtaac
     1621 gcttgtctgt ccataaggaa gtgggtctct ccaatgcaag gtcgcttgat tgccaatgag
     1681 atccaggagg cattgtccca agcaatctaa agcggccgga gaaggttgat agctataggg
     1741 cacaatgtca atttggatac cttgtccgcg ttcagtactc agcagctgca gataatacgg
     1801 ctcatccaga gggagcgtaa aggattgagc ggctttgtgt gcgaatcgtg cggatagcca
     1861 ggccagagtt acccgaggta tgacgatact cacaaaggcc gtcagtgccc acatgtgaat
     1921 ccactgtgca gcagagccct ggccgggagc ttgaaggtgt gcaaggtctt cggctgatgg
     1981 aaagggaaaa cccaggagcc aggctgctgg tcccaagagt ccgtataaaa ccgtatgcac
     2041 ctgtgccgct gataagaagg tgctttccca cgtggcttga taatctagaa ctagtccacg
     2101 gacgtacata ctgaggatga tgcctagagc cagacaggct gatccaatat gcagcatatg
     2161 acgaacgcga ctgataatga cgggatgcca gtgcctccac cacaggttca taaatcgttc
     2221 ggaagattga agaacccatt ctttgaccgc acctccaggt aaagcaattt ttggccatgg
     2281 tcctttcccc aaccattccg ccacggtggc aaatcctttg gccaggtgac ggagaagtgg
     2341 cccggttagg tcctttccca gaagcggcgg cagaatggta cccgcatagg tccccatatt
     2401 ccacaaaagc agaatcaaga gtggaaagtt taagagattg actcgttggg tcgttccgag
     2461 cccattgata aacaggccga cagcaaaggc cggtatcgcc acgatggtat atggaattgt
     2521 agcttgagcg cctcgaaacg attccgtgat catggggtaa gaggtggaga gaaaattcca
     2581 gagagattcc gcgcgattgg tgaaaaattg gatagagctc tctgctgatg agggtggttc
     2641 ctggggcgag cttttttttg cctgttggct ggctcgctgt ctggtggagt gtgagatgta
     2701 tcgtccttgt gggtccgatt gttcaagcgc ctggattaac agaatttttg ttacctgttt
     2761 cgaattcata actatagcca ttttggcatc tgaatctgtg gttttttcag gatggggtca
     2821 caaggcaaac agcctgccag gcttgaccta caaccatgat tgatctgact gttgtgtctg
     2881 tgtagaaggg gacgtgtttt gttgtgtgat aaatagctaa ttgcatgttg tgaggtcaac
     2941 ccagggaaaa cgggagacgc agtctatcct tagtttgtat ggctcaggta tggagggaaa
     3001 ccgaaccggc atgttttgac tcggaagaag aagcatccgg ttgaggctcc tgggaattat
     3061 tgtttacgga ttgggaggcg gaggcctcag aatctaacaa gatgctgatc caacgggtcg
     3121 aggggctgct ttccaaggca attttcacca ccatggtcat gggaacagaa agcaacattc
     3181 caactggtcc ccacacccac ccccaaaaca ccaaggagag aaagacaaca agagtggaaa
     3241 ggttaagttt cctccccata agtttgggtt ccactacatt accgaagatc aagttgacgg
     3301 ctacataccc cgcagctacc agcaacaggt gtccaatgcc gaattgaatg tatcccaata
     3361 gcattggcgg aactgccgca atgatagacc cgaggtttgg aataaagttc aacaaaaatg
     3421 ccagaagtcc ccagataagg ggaaaatcaa tccctaaggc ccacacccac aacgcgacaa
     3481 tcacacccgt tccaaggctg accacggttt tgatagctaa ataattgttg acggcgtccg
     3541 ttattttttt ggcctgggag aaggtgcctt ttggatctcc gaaggcggtt ccgactttct
     3601 cttggaagct ggacgcctca aataaaatga aaatgacagt gaggagaatt aaaaaagaat
     3661 tcgtgagcac gtttcccaac ccggacaaca ttttggctac gagcctcatt gaagccgccg
     3721 gatcgatgga tttcagtaat atccctttat caagttgaaa acctaactcc tggatccaat
     3781 ggatcatggg ctcgatttct tgggtcaatc gagcttggta gcgtggaatg gcttcggaga
     3841 agtcatccag ggaggttcca ataatcagaa taaacacaat cccgacgctg agaactccta
     3901 ggacgacacc gaacaccgcc aatggcgaag gtatgccttt ttgtcgaagc cagaaaacag
     3961 gtgatgcact gataatggca agaaatattg agacaataag agggaccagg atgggctctg
     4021 cagcccgcat cccagccaca actatgataa atgcggcggt ggtcaacaag aatcgaccag
     4081 ttgggggatg actggagggc ggtagttggg aattctgctg gttgtgcata atgaaaattt
     4141 ttatcgcgaa gaaaccatta cttctttggg tatgataccc gttttttcct ctcctgtctt
     4201 cgagagaaaa gttgtcggtg ctatggataa aagtcagggg agataaagaa aattcaagag
     4261 tctctaaact cttagggtgg tgattgctga gatgaccaag atgatgggct agaggactat
     4321 tcctgaatgg ccttccgccg ttgctggaga taaccattcc gaacggcacc atacaaatcg
     4381 agcgtgcctt cctcaacgcc ttgaaatttt tccagcgtga gcgcacggag attcaccgtt
     4441 tcgccggcga ccattccaag ccgtgataag gtgacggtca tatcgtgttg gaccaaccgc
     4501 ggagcgtcag cgatttcaat aaagggcagg actagccagt taaaagggtc aaggaaggaa
     4561 tcgccgatga atccaatgcc atctcgtaag gtgaagggcc ccaggagcgg aatcatgagg
     4621 aacggtccag gcgggacacc ataggttcct aaggtttgcc caagatcctc ttgcggagtt
     4681 tctaactcga acataattcc agccgggtca aacaatcctc cgaccccaag ggtggaattg
     4741 ataaggaatc ggctcagctc aatgccagct ccctgaaact ttgcttgaaa aatgttgttc
     4801 aataatctcg ggacaaatcg aatattttga aacacgtttg agaagctttg ctggacatcg
     4861 ggagggatga aaaaattgta aactttggca accggcttca ccacgtattt gtccaaccgg
     4921 taattaaatt caaagaccgc agaattaaca ggttcaaacg gatcatagtc cttattctgg
     4981 gaatcaccag attcgtcaaa aggatcaaag aattcatttt cttcgccttc agcgttgagt
     5041 ccgtcctcaa attcagcgat ttgaatgtcg ggtccggttt ccattccatc ctgggtcttc
     5101 gtggatttgg actttcccgc acatccacca agtcctatcg cgaggcatag aatgacccag
     5161 atggacaatc gcatcgtttc tcctctcatg ccaaaggctt ttcttttaag atttatatcg
     5221 ggcccgtcca cttcaatccg atcgttggaa cgcaagatcg aatcagtcat gtggaataac
     5281 ttcaactatt tcacagataa accgcgcgga ctgtaacaaa atcccatacc caagccaaga
     5341 atctctttcc tcgtccccac atcttggagg acctgtgagt ggtgacaaaa ttcaggcctt
     5401 tttcgacttt tttggtaaag gcatggaaaa ggatgctgaa ggttttgtca gcttccttga
     5461 caaaccatta caggaaaaat cgagaaaaag gtagatgctg accattcttt gtcctgatgt
     5521 ttctcagagg tcgtaaaccg tttcgacgtt ttcaataggg actttcttct tagagggaat
     5581 aggttcctca ttattggagg ggagtcaaca ttaacgataa tcaatgagca gcatgcgaca
     5641 aagctttttc agtggcactc cctttgctga ttcatcgcag gtgatcaaca aggttcacgg
     5701 ggtaccaatg cctcttaacc agatggtcaa atgatctgag aacgttcaat tgaagggaga
     5761 ccacctatga gcccatcggt atcggagcag aaaccttcct ggaatggttc cattaaacga
     5821 gtgaaaaaac aggaccgtca tcctgtctcg tccggcggcg gctctcctga agcggaccgt
     5881 tcgtcaggag tgagtgacaa gcaacttccc ctatacctaa aggaaatagg gcaagtgtca
     5941 ttacttgacc gggagggcga agttcgtctg tgcaagaaaa ttgaggaagc ccggcgaagt
     6001 atgttggaga ttctgtactc cctccctatg accttggact atcttcaaga acaacgcatg
     6061 cggttgttga atggcgaaat cctggccaaa catgttgttc aaagggaaaa agaagtggat
     6121 gtggagaccg aatcggagga agaacctgac attccagatg ttcaaaccga acagcaggag
     6181 gatgaagaat ttcgtcaacg ggtaattcag caactgagcc acctttgtca ttgtgttcaa
     6241 ttcctgatgg ccaaggaaca tgtatctgca tccaagcaag gcgcttcggc ctcaatgcaa
     6301 gccagaaaaa aattatggca aagtcttgaa gaagtgaatt ggcatcctgc atttaccaaa
     6361 caggtagaaa gccgcatacg ggcaacggaa cgccaactta ctgaagtatt gaaacgatta
     6421 ggattatccc ctcaagatat ttttaatggt gatgagcgaa cccgtggggt gggtcacaat
     6481 tctctacccg ccataaaccc tggcgttaag gtaaactcag gttcccatcg ttccaccctg
     6541 gaggctggag aacacctcgt atatttggag caagaagttt tgcgcctgtg cttttcagaa
     6601 ttcctcgcac gggttcggca attggatcaa gctaaaattc gcctgcattt cgccaaggag
     6661 gcgatgttgg aggcaaactt acgcctggtt gttagtgttg ccaagcgtta tgtgaatcga
     6721 ggcctcgatc tactggacct catacaggaa ggcaatatcg gcctgatgcg tgcagtagac
     6781 cgttttgagt atcagcgagg ctataagttc agcacctatg cgacgtggtg gattcgacag
     6841 gcggtaacga gggcattggc cgatcaatcc aggactgtgc ggattccggt gcacgtgtgt
     6901 gatgtgctta ctcgagtgcg acgaacgctg gatcgtttag cggtgcaatt ggggcgtgaa
     6961 ccgaaattgg aggagctcag cagcgcggtg gatattccac tggataaatt gtccacgatg
     7021 ttggaagcga caaaagggac tctgtcgctc gaaactccta tgggcgatga ggacggttcg
     7081 ccgctctctg atctcttaca agatcctaca gccgtttctc catgttattc ggcggaacgg
     7141 gtggatctcc aggaaaaagt gacttacttg ttagggactt taacacctcg ggaggcgcat
     7201 attattcggc gacgctttgg tattggagag cttgaggatg ctactcttga agagatcggc
     7261 cttgaattct ctgtgacccg agaacgcatt cgacagattg aggaacgggc cttggccaaa
     7321 ttgcgtgaac cccaacggaa tgtggtcttg cgtaattttt ttcaaccatc cggtccagct
     7381 ttagattagc ccgatacttt tcgtgaacgt gtgatgtcga accaagtagg cgactgattt
     7441 caaagtagaa caatattttc aaagtcactt aagggaaaag aggatcaccc gagggtggtg
     7501 aagaatccct cattgga
//
LOCUS       DATIAL010000500         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_519664, whole genome shotgun sequence.
ACCESSION   DATIAL010000500 DATIAL010000000
VERSION     DATIAL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519664"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..621)
                     /gene="infC"
                     /locus_tag="VLA60_15365"
     CDS             complement(103..621)
                     /gene="infC"
                     /locus_tag="VLA60_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HSF10789.1"
                     /translation="MTRPVTLKQRINHLIKVPEVRVIGAEGEQLGILKTSDAIRQAKE
                     IGYDLVEVAPTADPPVCRIMDHGKFKYEQSKKEHRMRLNQKSTQVKEVKFRPRTDKHD
                     METKVRQIRDFLEDGNKTKVTVMFRGREMANQELGFKAIEKVIEELKGAGNIESPPRM
                     EGRNLYMVVAPK"
     gene            complement(618..>2436)
                     /gene="thrS"
                     /locus_tag="VLA60_15370"
     CDS             complement(618..>2436)
                     /gene="thrS"
                     /locus_tag="VLA60_15370"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HSF10790.1"
                     /translation="ANGVEKDLLAPLETDVTLQPLAFDSPEGKEIYRHSSTHIMAQAV
                     KECFPTAQLTIGPAIEEGFFYDFSFDRPFTPEDLEVIETRAKAIIASNLPVIRLEMSK
                     AEAIDYFRQSGEHYKVELLEGIPDDQVSLYQQGEFVDLCRGPHVPSTGHIKAFKILSS
                     AGAYWRGDERNPMLQRLYGTSFPSEDALQAHLHNLEEIKRRDHRKLGKDLDLFSIQDE
                     TGPGLILWHPKGAQVRLLIENYWREQHLAHQYELVYSPHVAKLDLWKTSGHVDFYKEN
                     MFTPMPVEASEYQLKPMNCPFHIMVYKSHLRSYRDLPIRYGELGTVYRYERSGVLHGL
                     MRVRGFTQDDAHLFCRPDQLAEEVQKVLKFITSMLGTFGFTEFKMFLSTRPEKAVGTL
                     DQWNQATRALEGALKVGGYSYQEDPGEGVFYGPKIDVKIQDALGRSWQCSTVQIDFNN
                     PQRFGLTYVGEDGKPHQPIMIHRALLGSIERFFGILLEHYGGAFPVWLAPVQIMVLPI
                     SEKHQAYAQEVEKKLREEGCRVALDLRNEKIGLKIREAEKAKIPFMIVVGDREAEAEK
                     VSVRQRNGKNLGTMSALDVVTLIRDDMPEAVRKGTFLFE"
BASE COUNT          599 a          656 c          515 g          666 t
ORIGIN      
        1 taaaccgttt actagcgcct gagtgatttt ttaattttag tttggccacg tcgtgtttcc
       61 tctcccaatt atcaacaagt tgcgagtttc ttcaagggtg gattacttgg gagctactac
      121 catgtagaga ttccttccct ccattctcgg aggactttca atgttccctg ctccttttaa
      181 ttcctcaata accttctcaa tcgctttgaa cccaagctct tgattggcca tttcccgccc
      241 gcggaacata accgtaactt tcgtcttatt cccgtcctcc agaaagtccc gtatttgccg
      301 gaccttcgtc tccatgtcat gtttgtccgt ccgaggccgg aatttaactt ccttaacctg
      361 ggtggatttc tggttaagtc gcatgcgatg ttcttttttg ctctgttcat acttaaattt
      421 tccatgatcc ataatgcgac agaccggagg gtctgcggta ggagccactt ccaccaaatc
      481 atacccgatt tccttcgcct gccgaatcgc atcagaggtt ttcaggatgc caagttgttc
      541 accttcagca ccaatgaccc ggacttcggg aactttgatg agatgattaa ttcgctgttt
      601 gagcgtcacg ggtcgagtca ttcgaaaaga aaagtgccct ttcttacagc ctcgggcata
      661 tcatcccgaa taagcgtcac aacatcaagt gcagacatcg tccccaaatt tttcccgttt
      721 ctctgtcgaa ccgaaacttt ctctgcctca gcttcccgat cccctacgac aatcatgaaa
      781 ggaatcttcg ctttttcagc ctcacggatt ttaaggccaa tcttttcatt tcgcaagtcc
      841 aaggcgacgc gacagccttc ctctcgaagt tttttttcaa cctcttgagc ataagcttga
      901 tgtttttctg atatggggag caccataatc tgaacgggag ccagccaaac cggaaaagct
      961 cctccatagt gctccaggag aatcccgaaa aatcgttcga tagatcccag taaggctcga
     1021 tgaatcatga tgggttgatg tggtttgcca tcctctccca cataggtcag cccgaatcgc
     1081 tggggattat tgaaatcaat ttgaacagtg gagcattgcc aggaacgccc taaggcatcc
     1141 tgaattttca catcaatttt aggaccgtaa aacacgccct cccccggatc ttcctggtac
     1201 gaatatccgc cgactttcag ggcgccttca agcgcccgcg tcgcctggtt ccactgatcg
     1261 agggtcccca cggccttttc cggcctggtc gataaaaaca tcttgaattc ggtaaacccg
     1321 aatgtcccta acatgcttgt gataaatttc agaaccttct gcacctcttc cgccaattga
     1381 tccgggcgac agaataaatg ggcatcatcc tgggtaaatc cccgcacccg catcaggcca
     1441 tgtaagactc ctgatcgctc ataccgataa accgttccca attctccata tcgaattggc
     1501 aggtcacgat aactgcgtaa atgggattta tacaccatga tatgaaacgg acaattcatg
     1561 ggctttaatt gatattcact cgcctcaaca ggcatggggg taaacatatt ttctttatag
     1621 aaatccacat gaccgctggt tttccataaa tccagcttcg caacatgagg ggaatacacc
     1681 aactcatact gatgagccaa atgttgttcc cgccaataat tctcaatcag cagacggacc
     1741 tgcgcacctt tgggatgcca cagaataaga ccgggtccgg tttcatcctg aatgctaaac
     1801 aaatcgagat ctttccccag cttacgatga tcccggcgct tgatttcctc caaattatgt
     1861 aaatgggcct gcaaggcatc ttctgagggg aaggacgtgc cgtagagccg ctgcaacatg
     1921 ggattccgtt catctccccg ccagtaggct ccggcactcg acagaatttt aaaagcctta
     1981 atgtgtcctg tggagggaac atgcggccct cgacatagat ccacaaactc tccctgctgg
     2041 tacaaggaca cctgatcatc tggaatacct tccagaagtt ctactttgta atgctctcca
     2101 ctttgacgga aataatcaat ggcctcggcc ttggacattt ccaaccgaat caccggcagg
     2161 tttgaggcaa taattgcttt ggcacgtgtt tcaatgacct cgagatcttc gggggtgaag
     2221 gggcgatcaa atgagaagtc ataaaagaat ccttcctcga tggccggacc gatagtcaat
     2281 tgtgcagtag gaaaacattc tttcacggct tgggccatta tatgggtact actgtgacgg
     2341 taaatttctt tgccttccgg cgaatcaaat gccagaggct gcaatgtcac atcggtttcc
     2401 aacggcgcca gcaaatcttt ttccaccccg ttggcc
//
LOCUS       DATIAL010000501         5666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_842792, whole genome shotgun sequence.
ACCESSION   DATIAL010000501 DATIAL010000000
VERSION     DATIAL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842792"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VLA60_15375"
     CDS             <1..1073
                     /locus_tag="VLA60_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HSF10791.1"
                     /translation="AGALISLASEKIAMADGGTIGAATPVQIGLPGSPAQPVEEKTVS
                     YMRKEFRATAEQRNRPPLIAEAMVDADVEVPDLIEKSKLLTLTTKEALQAKIADFQAN
                     SLEAVLQSVNLADAEIRYASETWAESLVRFLTHPVVSSLLMTVGILGIMLEMRMPGFG
                     VPGALGLISLALFFWGHGLVQLAGLEEFLLVGVGLILVGLEIFVIPGFGIAGILGIMA
                     LMGGLGLSLVGTGATWDFMLSALGQVALSILVAIFATLILLRYFPRLPFGKRLILETN
                     LQAQEGYESSPPEDHRWLGKQGIAVSDLHPSGIARFDRERVDVVSDGTFIDAGQPLEV
                     VRIDGNRVVVRLAPPQSEKDTV"
     gene            complement(1239..2294)
                     /locus_tag="VLA60_15380"
     CDS             complement(1239..2294)
                     /locus_tag="VLA60_15380"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10792.1"
                     /translation="MHKANISVSGEHYGLLYSPELMQSPTLAKSVFELQQFSCIIDQH
                     GFRNTIPLDQADIFTLGDSFTFGWAIDSARSWVGILEDAIHQPIYNLGILDSSPKQEL
                     ELLKYMIRTSGKSMRIRTLLWMIYEGNDLEDSTREKGWTKPNSRNNMKQLMEGTVLQS
                     LYQIPFLIKEQAMITKIRNKDIVFRLPSLQKDGSNPNVIDGIQSWYPLYHSATLGPRL
                     FIPEYIERAGKPSSYVLNHNNRPGLDKVFEEMAQLGKEYSFKVIVVIAPTAVRLHGRF
                     YENFPPISDNPHFIDYIATLSEQKGFRTLNLLPFLSTYGDKELLYLRDDDHWNKKGHA
                     VAAEIIFREIFQQGTEK"
     gene            3048..4022
                     /gene="floA"
                     /locus_tag="VLA60_15385"
     CDS             3048..4022
                     /gene="floA"
                     /locus_tag="VLA60_15385"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HSF10793.1"
                     /translation="MEIETQAFGSLGLLVMVLVLLFGLLYVIPIPLWIAAWASNAYVG
                     LFTLIGMRLRRVPPVTVINARISAVKAGLEIPVNDLEAHFLAGGNVVKVVNAMISADK
                     ANIPMTFKRAAAIDLAGRDVLEAVKMSVIPKVIETPRITAVAKDGIQLIAVSRVTVRA
                     NIDRLVGGAGEETVIARVGEGMVSTIGSSATHKDVLENPDYISKNILGKGLDAGTAFE
                     ILSIDIADVDVGENIGAKLQIDQANADKQIAQAKAEERRAMAVALEQEMRARVVEAEA
                     EVPKAMSEAFRQGNLGIMDYYRMKNVQADTAMRGAIAGTEEESPGPSK"
     gene            4030..4386
                     /locus_tag="VLA60_15390"
     CDS             4030..4386
                     /locus_tag="VLA60_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10794.1"
                     /translation="MMSLEAFISFTIFLFLSLIALAIRWLNERIRRDIALRDLKDLFL
                     SDGDSANQRAPETPDITNISPSAQQPPIQQFRPTTPPKRRTASRLRLRNKADLRQGII
                     IMTVLGPCRALEHPDE"
     gene            4565..>5666
                     /locus_tag="VLA60_15395"
     CDS             4565..>5666
                     /locus_tag="VLA60_15395"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HSF10795.1"
                     /translation="MSNEGVTTVSYPVRGPVLLKYLGQLGLMLAMLTLVPFGVSLFFN
                     NDTVAGRYLIIILALFFLGTPLSRVRVPPTLQTNEALAITAMAFLISPLVMTYPLMGA
                     GLSFEDALFEAVSGVTTTGLSVLPSVEDRTPSLIFARAWMQWYGGLGIAILSVALLMG
                     QGIASKRLVEPVPGDNFVTTTRTHARQVLGAYVLLTVSGIVILLTLQVELFHAITLTL
                     ASISTGGFSSFNDSLVGLESMPTASIVLFISFCGAISLPLYARTYRDGFQRLTSDVDL
                     PTLFLLCILTTGLISLSLGWHTNLTWPEAMTHGAALAVSAQSTTGFTILPLSDVDPTS
                     KSLLMASMVIGGSVGSTSGGIKILRFLMVLALF"
BASE COUNT         1349 a         1462 c         1402 g         1453 t
ORIGIN      
        1 ccgccggggc gcttattagc ctggcctccg aaaaaattgc gatggcagac ggcggcacca
       61 tcggtgccgc cacgccggtg cagatcggtc tcccgggttc accggcccaa ccggttgaag
      121 aaaaaaccgt ctcgtatatg cgcaaagagt tccgggcaac cgctgagcaa cggaatcgcc
      181 ccccgctcat cgccgaagcg atggttgatg cggatgtgga agttccagac ctgattgaaa
      241 aaagtaaact gttgactctc accaccaaag aagccttaca ggccaagatc gccgactttc
      301 aagccaattc gctggaggct gtgctacaat ctgtaaacct cgctgatgct gagattcgct
      361 acgcgtcaga aacttgggct gagtctctgg tgcggtttct cacacatccc gtggtcagct
      421 ccttattgat gaccgttggg attttgggaa ttatgctgga aatgcggatg cccggcttcg
      481 gggtacccgg cgccctcggg ctcatcagcc ttgccctctt tttttggggg catgggcttg
      541 ttcaattggc cggattggaa gaattcctac ttgtcggagt tggtctgatt ttggttgggc
      601 tggaaatttt cgtcatcccc ggctttggca ttgccggaat cttaggaatc atggcactca
      661 tgggaggact aggactcagc ctcgtcggga ccggggcaac atgggacttc atgctgtccg
      721 cattggggca agttgccctt tcaattctgg tggccatctt cgccaccctc attcttctac
      781 gctatttccc acgccttcca tttggaaagc gactgatact ggagaccaat cttcaagctc
      841 aagagggata cgaatcaagt ccgccagaag accatcgctg gctgggaaaa cagggaatag
      901 ccgtttcaga tcttcaccct tccggcattg cccgttttga tagagaacga gtggatgtgg
      961 tatccgacgg aacctttatt gatgccggtc aacctcttga agtcgtgcgc atcgatggga
     1021 atcgggtggt ggttcgactc gcaccacccc aatcggaaaa ggacacggtc tgatcgaacg
     1081 agaaggcttg gcattcatgc agctttcttg aaatcagtga ctattaggct gcgccctcgt
     1141 ggctcctcct ttcgtatcgc gccgtgacca tttcaagttt atcttagcct tcattttcca
     1201 tctctctttc tattcccaag aagaagccgt cacggttctc atttttcagt tccctgctga
     1261 aatatttccc ggaatatgat ctctgcagca acggcatggc cctttttgtt ccaatgatca
     1321 tcgtctctaa gatataagag ttccttgtct ccataggtcg agagaaatgg gagtagattg
     1381 agcgttctga atcccttttg ctcagaaagc gtggcgatgt aatcgataaa atgaggattg
     1441 tcggatattg gtgggaaatt ttcataaaac cgcccgtgaa gccttacagc cgtcggagca
     1501 atgaccacaa tgaccttgaa agaatattct ttccccagct gtgccatttc ttcaaaaact
     1561 ttatccaaac ctggtctgtt gttgtgattt aacacataag aggatggttt ccctgctctt
     1621 tcgatgtatt ctggaataaa caaacgcggt ccaagggtag cagaatgata aagaggatac
     1681 cacgattgta ttccgtcaat gacattcggg ttggacccat ctttttgcaa acttggaagc
     1741 cgaaaaacaa tgtctttatt tcgtattttc gtgatcatcg cttgctcttt aatgaggaag
     1801 ggaatttggt acaaactttg aaggacggtt ccctccatca attgcttcat attattgcga
     1861 ctgttgggct tagtccaacc tttttctctt gtgctatctt caagatcgtt tccctcgtag
     1921 atcatccaga gcagtgtgcg tattcgcatt gattttcccg aagttcttat catgtatttt
     1981 agtagttcaa gttcttgttt tggagaggaa tcaagtattc ccagattgta aatcggctga
     2041 tgtatggcat cttccagaat tccaacccag gagcgtgcgg aatcaatggc ccatccgaag
     2101 gtaaaagaat ctcccagtgt aaaaatgtcc gcttggtcca atggtatagt attgcgaaat
     2161 ccgtgctggt cgatgataca agaaaattgt tggagttcga atacagattt ggcaagagtc
     2221 ggtgattgca tcagctcggg actatacaaa agcccataat gttccccact gaccgaaata
     2281 ttggctttat gcaatcggaa attgcgttcc gtgggatagt agagaggagg atacaactcc
     2341 gtccaggtca tcgcagagtc gaatcgtctc gattcctcca gacgaggttt tccgatggac
     2401 tgggacttga ggtgaatagc atatatgatg tccaaaatga agaggctacc caatgacaca
     2461 cacactacta ggctgaggtt tacaagctgc tttgaggaga ccaccccgcg aaccatcatt
     2521 gctcgaatga ccatgaagaa cacgccaata gcaaacggaa gggagaggcc tatcaggccc
     2581 agccctatgc ccttcccgcc gatagaccgg cctaagaaca ggaccagcac aaaatataac
     2641 cacaacaacc caagactggc agtcgcgaac accatggcgt aggattctct tttattcata
     2701 ggttcccttt cagggataag gccttttcat catggtcaaa aagaaagagg gtcctggacc
     2761 aagatggaga tgtccccata ttaacatcca cctgatatct gttgtttttc attcaattgg
     2821 aacggcatac gaatctagaa gggtatgagg gagaaaggct aaaaattgcg ccgaaatcct
     2881 tgaccattgt tgttatactt tatactggtt ctctgtcaag gctgtattag aatcaaagtc
     2941 ttatccatga agtcccggaa aattcaccaa tcggaaatca tttctgtgca cgtttcccat
     3001 gtttccttct ttgacgaatt ttcacaatca gaaaaggacc acgaataatg gaaattgaaa
     3061 ctcaggcatt cggttcactc ggactccttg tcatggtcct tgtgctctta tttggccttc
     3121 tctatgtcat tccgatccct ctgtggatcg cagcatgggc ttcaaacgcc tatgttggac
     3181 tctttacgct cattggcatg cggttgcggc gggttccacc ggtcaccgtg atcaacgcca
     3241 gaatcagcgc ggtgaaggcc gggctcgaaa ttcccgtgaa cgatttggaa gcccactttc
     3301 tggccggagg aaatgtggtc aaagtcgtca atgccatgat ctcagccgac aaggccaata
     3361 tccccatgac ctttaaacgc gccgccgcca tagatctggc gggacgtgac gtcctggagg
     3421 ccgtcaaaat gtcggtcatt cctaaagtca tcgaaactcc ccgtatcacc gcagtcgcca
     3481 aggatgggat ccagctgatt gccgtctcac gagtcaccgt tcgcgccaat attgaccggt
     3541 tggttggcgg agccggtgaa gaaacagtca ttgcgcgggt gggagaaggc atggtgagca
     3601 ccattggctc atccgccaca cataaggacg tgctggagaa cccggattac atttcaaaaa
     3661 atattttagg gaaggggctc gatgccggaa cagcttttga aattctgtcg atcgatattg
     3721 ccgatgtcga tgtgggagaa aatatcggtg caaagctgca aatcgaccag gccaatgcgg
     3781 acaagcaaat tgctcaagcc aaagcggaag aacggcgggc gatggcggtc gcattggaac
     3841 aggaaatgcg cgcccgtgtc gtcgaggcgg aagcggaggt tcccaaggcc atgtctgaag
     3901 cctttcgtca gggaaattta gggatcatgg attattatcg gatgaaaaat gtgcaagccg
     3961 atacggctat gcgaggagcg attgccggga cggaagagga atcgccggga ccttcaaaat
     4021 aatgaggtaa tgatgagcct ggaagcattt atatctttca ccatttttct ttttctatcg
     4081 ttgatcgccc ttgccattcg atggctgaac gaacgaatac ggagagacat cgccctcaga
     4141 gatctgaagg atctctttct ttcagacggt gactccgcaa accaacgtgc ccctgagaca
     4201 cccgacatca cgaacatttc tccgtcggca caacaaccgc ccatacagca attcagaccc
     4261 acgacccctc cgaaacgccg gaccgccagc cggcttcgtt tacgcaataa ggcagatctt
     4321 cgacagggga ttatcatcat gacggttctt ggtccctgcc gggcgctgga acatcctgat
     4381 gagtaaaaca ggggagcgtt gaacaatgcc gccatcggag ggcacggcaa cgatctgtcc
     4441 aggaaccata gaatcgcggg aaaccgcttt tcctccgaat tcaacctgcc attcaacact
     4501 cattttccta tccatcccgg caacgaaatg gtctatttga atggggctca ttgaggcata
     4561 accgatgtcc aatgaaggtg tgaccacagt cagctatccc gtccgtggcc cggtccttct
     4621 gaaatactta ggccagctcg ggctgatgct ggccatgctg accttagtac cgttcggtgt
     4681 atcactgttt ttcaataacg atactgtcgc tgggcgatac ttgatcatta tcctggcatt
     4741 gtttttcctc gggacacctc tctcccgcgt acgggttcct cctactctcc aaacgaatga
     4801 agccctggcc atcacggcca tggcctttct gatctctccc ctggtgatga cctacccgct
     4861 catgggagca gggctgtcct tcgaagatgc gttgttcgaa gccgtctccg gcgtgaccac
     4921 aaccggtctc tccgtccttc cctcggtgga agatcgtacc ccgtcattga tatttgcccg
     4981 ggcatggatg caatggtacg gcggattagg catcgccatt ctttcggtgg cattgctaat
     5041 gggacaaggc atcgcctcca aacgattagt ggaaccggta cctggagata atttcgtgac
     5101 caccactcgg acccacgccc gccaggtcct tggcgcctat gtcttactta ccgtttcggg
     5161 gatcgtgatt ctcctgaccc ttcaagtgga acttttccat gcgataacct tgacgctggc
     5221 atccatctcc accggaggct tttcctcatt caatgacagt ctggtcggac tggaatcaat
     5281 gccgacggcc tccatcgtgc tgttcatttc cttctgcggg gcgatttcac tgccactcta
     5341 tgcccgcact tatcgcgacg gatttcagag gctgacatcc gatgtggact tgccaaccct
     5401 ttttcttctt tgtatcctga ccaccgggct catcagcctt tcgctgggat ggcataccaa
     5461 tctgacctgg ccagaggcta tgacccatgg cgcagccctg gctgtttcag cgcaatccac
     5521 gacgggattt actatcttgc ccctttccga cgtggacccg acctccaagt ctctgctcat
     5581 ggcttcgatg gtaatcggag gaagtgtggg ctctaccagc ggcggaatta aaatactccg
     5641 gttcctgatg gtgctcgcat tgttcc
//
LOCUS       DATIAL010000502         3663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_842867, whole genome shotgun sequence.
ACCESSION   DATIAL010000502 DATIAL010000000
VERSION     DATIAL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842867"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VLA60_15400"
     CDS             <1..579
                     /locus_tag="VLA60_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10796.1"
                     /translation="YLAKKIADASGTRIVAVVGAGHVEGICRTLHGREQVDLEELESI
                     QPVSPVWKAVGWSIPVMIVGSIAWIGWQKGLSAAGENLLFWILANGIPSGIGGLLAMA
                     HPLTIAAAFISAPFTSLTPVIGVGYVTAFVQAYLQPPLVREFQTVAEDIAIPRKWWKS
                     RLLRVFLAFLLPTIGSLIGTWVGGTRIVSNLF"
     gene            835..1593
                     /locus_tag="VLA60_15405"
     CDS             835..1593
                     /locus_tag="VLA60_15405"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HSF10797.1"
                     /translation="MYTTMNASKIVNGVVLGLLLLLPVSGWGGTNDEDLPRLTVSAEG
                     TINVSPDKAVLSFAVETVGEKLSDVQKENQERVAKVLEECRRFNIPSEFIQTTSLNVI
                     PEYPPPPRRPTDGTLENSVPRIIGYRVVHHVNVEVRNLDVVGKVVDRVLQVGANRFSG
                     IAWGLQNEQPTKLEVLKQASAQAQSKAEALAQALNLKLVRMINVSEGGVSPSPPEGRY
                     RMAMAMDSGGDASVSAGEISIRGSVLLVYEISQK"
     gene            1654..2583
                     /locus_tag="VLA60_15410"
     CDS             1654..2583
                     /locus_tag="VLA60_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HSF10798.1"
                     /translation="MNNEKSEAVARTGKAGADLGVPALVVSGFLGAGKTTLVKHLIAD
                     AQRQGLKLAVISNEFGELGIDRALLQEEGGPGYVELEGGCVCCQLSGELLNTLQNLWE
                     TIRPDRVVVETSGVALPFETLLTFWREPVTEWVGESLAVVVVNAEQVAQGRDLEGTFE
                     QQVSSADLLVFNKVDLVPADSIGRLENLLQDMAPGAPVIRSIQGEIDTTIVFPCLPAK
                     KVSPNSQRKPELLPHTHEEFEAEELSFSSDLTSDQLIQQLQSLQALRIKGIVNTSEGP
                     KLVQGVGSRIDLSSPPSQFPKEMLGRLVAIRRK"
     gene            3184..3480
                     /locus_tag="VLA60_15415"
     CDS             3184..3480
                     /locus_tag="VLA60_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10799.1"
                     /translation="MQYNLLIKRTILVASLALIPMILPGLTFAAGQEKERKSSSEVQT
                     HGLTQIAVGSVNDTLQLCLGRIPQDASAGQLLLAEQNCQQVDVDRTINQASLTF"
BASE COUNT          845 a          867 c         1001 g          950 t
ORIGIN      
        1 tatttagcca aaaaaattgc cgatgcgtca gggaccagga ttgtcgccgt ggtaggcgcc
       61 gggcatgtgg aaggcatttg ccgcacattg catggccggg agcaggtgga cctggaggag
      121 ctggaaagca ttcagcccgt ctctccggtc tggaaagcag tgggttggag tatcccggtt
      181 atgattgtgg ggtccattgc ttggattgga tggcaaaaag ggttaagtgc tgccggagag
      241 aatttattgt tttggatttt ggccaatggt attcccagcg ggattggcgg gttgttggcc
      301 atggctcatc cgctcaccat cgccgccgca tttatctcgg caccgtttac cagtctcacc
      361 cccgttattg gagtcgggta tgtgacagcg tttgtccagg cctatttgca accgcctctg
      421 gtgcgggagt ttcaaacggt ggcggaggat attgccattc cacgaaagtg gtggaaaagt
      481 cgactccttc gtgtattttt agcctttctc cttcccacga ttgggagcct catcggcacg
      541 tgggtagggg gcacccgcat tgtttccaat ttgttttaat caggagccaa acaaagcaac
      601 gcaccgtgag tttagggttg gggcgcgggg actttcgacg tatgctggaa aatacatttc
      661 gggcaggtat agtgaataaa tcgaccatcg ccaacgagac gtttcagcat agaggtttga
      721 caccaggtgc agagacggtc cggcagatcc gaggcagaag gcttcaaagg aggaatagaa
      781 tcggtgttgg tcataactag aagccattct caaaaacatt cagggggttt gtcaatgtat
      841 acgaccatga atgcctcaaa aatagtgaat ggtgtggtgc tgggattgtt gctgctgctc
      901 ccggtatccg ggtggggggg aacgaatgat gaggatcttc cacgattaac cgtttccgca
      961 gaggggacga tcaatgtgtc tcctgataaa gcggtactga gttttgcggt cgaaacagtc
     1021 ggagaaaagc tgtccgatgt tcaaaaggag aatcaggagc gggtagcgaa ggtcttggag
     1081 gagtgccgac ggtttaatat tccgtctgag ttcattcaaa caacgtcctt gaacgtcatt
     1141 cctgaatatc caccaccccc gcgacgtcca accgatggaa cgttagagaa cagtgttccc
     1201 cgtatcatcg gctatcgtgt cgtccaccac gtcaatgtgg aggtgcgtaa tcttgatgtg
     1261 gtgggaaagg tcgttgaccg ggtcttgcag gttggagcca acagattttc aggcattgcc
     1321 tggggcttgc aaaatgaaca acccaccaag cttgaagtct tgaaacaggc ttctgcccaa
     1381 gcccaatcca aagctgaagc gttggctcag gccttgaacc tcaaattggt tcgtatgatc
     1441 aatgtctcgg aaggtggcgt ttctccttcc cctcctgagg ggcgataccg catggcgatg
     1501 gcgatggata gcggtggaga tgcatcggtg tcggcaggtg aaatctcgat acgtggttcc
     1561 gtcctcctgg tgtatgaaat cagtcagaag taatcaattc tgtctttatc cgactccgga
     1621 ccccggtcct tagtgctcat gagcattccc tccatgaaca atgaaaaatc agaagctgtt
     1681 gcacgtactg gaaaagctgg agcagatctt ggcgttccgg cattggtggt gtccggcttc
     1741 ctgggggcgg gcaagacgac acttgtcaaa catttgattg ccgatgctca gcgacagggc
     1801 ttgaagttgg ctgtgatctc caatgaattt ggtgaattag gcattgatcg ggctctgctt
     1861 caagaagagg gcggtcccgg ctatgtggaa ctggaaggcg ggtgtgtctg ctgccagctt
     1921 tccggtgaat tactcaacac gttgcagaat ctgtgggaaa ccattcgtcc ggatcgagtc
     1981 gtggtggaaa catccggcgt ggcattaccc ttcgaaacgc tcctgacatt ttggcgtgag
     2041 cccgtcacgg agtgggtggg tgagagttta gcggtggttg tcgtcaatgc ggaacaagtg
     2101 gctcaagggc gggacttgga gggcacgttt gagcaacagg tatcttcggc agatctgttg
     2161 gtcttcaata aagtcgattt agttccagca gactcaatcg gtcgattgga aaaccttctt
     2221 caggatatgg cccctggtgc tccagtgatc cgaagcattc agggtgaaat cgatacgacg
     2281 attgtttttc cttgcctccc cgcgaaaaag gtttcaccca actctcaacg aaagcctgaa
     2341 ctccttcctc acacgcatga ggaatttgag gcagaggaac tctctttttc tagcgacctc
     2401 acgtctgacc agctcattca acaacttcaa tcactgcaag cccttcgcat caaaggcatc
     2461 gtgaacacct cagaaggtcc gaagctggtt caaggcgttg gttcccgcat tgatctgagc
     2521 tcgcctccct cacaatttcc caaagaaatg cttggccgcc tggtcgccat ccgtcgaaag
     2581 tagggatttc ggttctttca acgaaaatag agatttcgga ttttccaatc tctccaggga
     2641 tcgggttctc attcatgcag gacttgtcgg aatcattagc ttttattgtc gactacctca
     2701 acgacacgca gggtgttgcc ttgacaccct cctgtttaag gtatagcgtg agtatatgac
     2761 gtatgaatct acgttgactc tcctatccct cgcgcttcaa ctttccttcc tgcgcgtctg
     2821 attcttctgt ctccgcaagc ctacgcctaa actcttctca ccaatcggtg ccgatcaacg
     2881 gcttcgattg taacgctggt cgtcagatca ggttccggga gagttcattc ctgatcctgc
     2941 gatctataag aaaagaggtg ggtatcatgt atccattcga agttctcttg ggcattatgt
     3001 ttctcttttg gggcgcggct atttatgcaa cttttacaga aaggccatct gtaaaagggt
     3061 tggtcttcga gggagtataa atcttctccg ttggcgatgc gcctatcttt gacgacgtgt
     3121 cacgatgatc cgtgcgactg cagatcatca ttttcaccat ttttcaagaa agagagaaaa
     3181 gctatgcaat acaatttatt gatcaaacgc accatcctcg ttgcctcctt ggcgctaatc
     3241 cccatgatcc tgccaggact gactttcgct gcgggacagg agaaagaacg gaagtcctct
     3301 tctgaggtac aaacgcacgg tcttacgcaa attgcagtgg ggagtgtcaa tgacacatta
     3361 caattatgtc tcggcagaat tcctcaagat gctagtgccg gacagctttt gttagcggag
     3421 caaaactgcc aacaagtcga tgttgaccgg actatcaatc aggcttcgct gactttctga
     3481 ggccaatgca gaatgccact gccctttggt tgacaaggaa ttatgctggg gaggtctctc
     3541 agtactggct ctgagccgtg ggtgatggag ttgttgcccg tgttcggcgt cagctcagcg
     3601 ccttatctca tcttttccct cagaagggaa agggtgagat gggacagcca caagagtaga
     3661 ttg
//
LOCUS       DATIAL010000503         3438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_950822, whole genome shotgun sequence.
ACCESSION   DATIAL010000503 DATIAL010000000
VERSION     DATIAL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950822"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VLA60_15420"
     CDS             <1..897
                     /locus_tag="VLA60_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HSF10800.1"
                     /translation="NHIGDGVTLVLISLGIGAVGFRFVWPAWKSACIHTLLAHGVAGL
                     FTQILKHTIGRPRPRLKQGQDWEIAPSLESGWDSFPSGHTTASFAVATVLAYHFPKGK
                     MLWFGLAAFNGTCRVITGAHFPTDVLGGVLVGIGTALIIIHPPDQWKAFFQQTLTHGL
                     PWLITAFGIVWIIVPHPGVELEPSLSLFLGLIGIAVGLGLRLWWIRELTSEETPSPGR
                     ELPQWPRLLMGLGLATTTGSIVILGASLMAGIIWWRGTQFELTPEIKNSFLNGFNPIW
                     IEAIIGLVMFFLTILIFSIRSA"
     gene            complement(1115..1477)
                     /locus_tag="VLA60_15425"
     CDS             complement(1115..1477)
                     /locus_tag="VLA60_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10801.1"
                     /translation="MKNVLKDKSIVLVGSLAGTCLMATVAFANPALLPDHAGYPMKES
                     KSPVSGVPTANDPGRENLYGQKALSAATKSYTEDLETRRSGAMGANESESQNQMGRQD
                     QKMGQEHMTSQDQKGHQK"
     gene            2475..3191
                     /locus_tag="VLA60_15430"
     CDS             2475..3191
                     /locus_tag="VLA60_15430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF10802.1"
                     /translation="MNQRSTPRGQTPKRSTISSLLILSVLLSLTASCAHKTWDINRYI
                     HALERPERDQHQQPEKVIEALNLTPGMMVIDIGAGSGYFTRRFAQSVGDKGRVIAIDV
                     EQKMLNYNKQQLEKLGLANRVHFVLAESEGPTFSGNNADMVFLCDVYHHLEDQIDYLA
                     NTKPALKPNGRVVIIDYYNDARSGTLGFSKRHLVPKEQVIKDMEQAGFTLAKEHTFLP
                     RQYFLEFVSKKGGTSARMEN"
BASE COUNT          851 a          906 c          753 g          928 t
ORIGIN      
        1 aaccatatag gagacggggt caccctggtc ctcatttcct tgggaattgg ggcagtggga
       61 ttccgattcg tttggcctgc ctggaagtcc gcctgtattc ataccttact cgcacacgga
      121 gtagccggac tctttaccca aatattgaaa cacaccatcg gccgcccccg cccgagactc
      181 aaacaaggcc aggactggga aatcgcccca tcacttgaaa gtggatggga ctcctttccc
      241 tccggtcata ccaccgcatc ctttgccgta gccaccgtcc tggcctacca tttcccaaaa
      301 ggcaaaatgc tctggttcgg acttgctgca tttaatggaa cctgtcgcgt catcaccggc
      361 gcccattttc ctacagatgt gcttggcggt gtactggtgg gaatcggaac tgcgctgatc
      421 atcattcatc cgcctgacca gtggaaggca ttctttcagc agacactcac tcatggtctc
      481 ccctggctga tcacagcctt tggtatcgta tggatcattg ttccccatcc aggcgttgaa
      541 ttggaacctt ccctctcact ctttctcgga ctgataggca tagcggttgg attgggacta
      601 agactctggt ggatacggga acttacctcc gaagaaaccc catctcccgg acgagagctc
      661 ccccaatggc cacgacttct tatgggcctt ggcctagcca ccacgacggg tagtattgtg
      721 attttgggag caagtttgat ggcgggtatt atctggtggc gggggactca attcgaatta
      781 acccctgaga tcaaaaacag ctttctcaac ggcttcaatc ccatttggat cgaagcgatc
      841 atagggctcg ttatgttttt ccttacaatc ctgatttttt caatccgatc cgcttaactt
      901 ctgccattca tgacagaatt tcagagcctt caacgattcc ctatcttgtg aaatctctcg
      961 cgaatcaacc cttctatgtt ttctgccgag gaggaccatc acagtccccc tcggactacc
     1021 tttataatcc ccggttcaga aaaatttcca tcaaccagag tcccaatgag ggtctcaaaa
     1081 ttgtccgagg tcctggattg gtctcttcat caatttactt ctgatgaccc ttttgatctt
     1141 ggctcgtcat gtgttcctgt cccatcttct ggtcctgtct gcccatctgg ttctggctct
     1201 ctgactcgtt tgctcccata gcaccggacc gacgggtttc caggtcttca gtgtatgatt
     1261 tagtggcagc acttaaggct ttttgaccat acaaattttc tcgccccgga tcattagccg
     1321 tggggacgcc actcaccgga cttttcgatt ctttcatcgg gtaaccggcg tgatccggca
     1381 atagcgctgg attggcaaag gcgactgtcg ccatcaaaca ggttccggcc aaacttccca
     1441 ctaatacgat agatttatct ttcagtacgt ttttcataat gcctccttga tttgatttag
     1501 ttgtatggct catatgagct cggcataccc ggaaatttcc tatatcctca gacttcattt
     1561 ttctcttcct ataacttcac cgtgaaatag tcccatgaag tatccagttt agacagaaag
     1621 aaaatgcttt cttgcaagcc tccttccaaa gcaataaaaa aattgaggat ccagcagata
     1681 gatgattaat gggaattaat ttcatcctat acctcatatg caaagccgta actgtcaata
     1741 acccttgaaa gccatttttc atgtatttct gttatgaatt gcccaaagga atagccaaat
     1801 ctatggcaaa gctgatctgg catgtcgggt gcggagagaa atgcctaagt gcaataaccc
     1861 atggaaaata tttacatttt cctacaaatt atcctggcgg caggctggtg ggattgtccc
     1921 aggactggcc attatccatc ttttcaacaa atgcatgaag gaggtctttt ggcaaaatct
     1981 ggccagaatc tccctggttg ataatgacct ttgctctgtt atgaattctt attccacacc
     2041 ctgaactcga atcagagccg tctttctcac tctccctcgg actgattggt agctatggtg
     2101 tctggctttg gactcagact ctggcggata catgaatggt cccacgaatg aaaccaatct
     2161 cacaggggtt tgcgccttca atggccgcgt ttccttatgg gccttggttg tgagaggcca
     2221 caacgggaag tgttgtggtt ctgggagcaa gtctggtggt tgaagtcgtc tggtgggtag
     2281 gaagtcccct cattccctcg catccatttg gagcagactc agatcattgg attattcaca
     2341 cttccatttg gactcctcat gtcattgctt cacctattac ttgaaactcg tctcttcaat
     2401 cgttataaaa gtaaaatgtt tgccatttac cccctatcca aaaaaaatta gacatgaatg
     2461 taaggatgat acctatgaac caacgctcga cacctcgagg acaaacaccc aaaaggtcaa
     2521 cgatttcctc tctcctgatt ctttctgttc tcctaagtct taccgcaagc tgcgcgcata
     2581 aaacatggga tatcaacaga tacattcacg cccttgaacg cccggaacgt gatcagcatc
     2641 aacagccaga aaaagtcatt gaggccttga acctcactcc tggcatgatg gtaattgata
     2701 tcggggcagg gtcgggttat ttcacccggc gcttcgcaca atcggtggga gacaaggggc
     2761 gggtcatcgc catcgatgtc gaacagaaaa tgttgaatta caacaaacag cagttggaaa
     2821 aacttgggct cgccaacaga gtccattttg ttctggccga gtcagaaggt cccacatttt
     2881 ccgggaacaa cgcagacatg gtgttcctgt gcgacgtcta tcaccaccta gaagaccaga
     2941 tcgactatct ggctaacacc aaacccgcgc tcaagccaaa cgggcgtgtg gtgattatcg
     3001 actactataa cgatgcacgg tccggcacac tcggcttctc aaaacgccac ctggttccaa
     3061 aagaacaagt catcaaggat atggaacaag ccggctttac cctggccaaa gaacacacct
     3121 tcctcccccg ccaatatttc ctcgaatttg tctccaaaaa aggtggtaca tccgctcgaa
     3181 tggaaaatta agcttgatgg gcctttcagc gtgcctacaa ggtcgtcagg cgccataggc
     3241 gcgaagttga ttgatttgtt atgtgattac cgcccacggt gccgctccat gttttgcagt
     3301 aagcaaccag cgtaatggcg tagaaaggga agaagctctt ttgcgcggaa ttttgccacg
     3361 aggctgccac cgttttcaga atcagaaaac tccaaccaat caacaaaatt ccgctcgctt
     3421 tccgatgtga cactaata
//
LOCUS       DATIAL010000504         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1489219, whole genome shotgun sequence.
ACCESSION   DATIAL010000504 DATIAL010000000
VERSION     DATIAL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489219"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VLA60_15435"
     CDS             <1..885
                     /locus_tag="VLA60_15435"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HSF10803.1"
                     /translation="DQPTVPGYSSYPQGQYPQQSNAYPNNQNAYPNQNPIPNQVPYSN
                     QSPYPENSYPQSQPEQSYSTPYGNSEIAPSPASPYPPEGSPGYQSTQPDTTQPAMVPI
                     SLDVLLVKKHLVNGVQSYLPIKDGDLLLDGRGNAKAGDKFRVMFRANTDCYVYVIAVD
                     GSAWAQGIFPTASSPFANPVKAGQQHVLPGGTNWFSLDQYKGIETIFFVASHARRPDI
                     EEILMTISGRERNPKEKPQAVNEPAIIPNGYSGVRPGSSPFAFQTSQHQQQELLPTTY
                     FVKTAGEDLRITRWFRHQ"
     gene            1435..>2547
                     /locus_tag="VLA60_15440"
     CDS             1435..>2547
                     /locus_tag="VLA60_15440"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HSF10804.1"
                     /translation="MLRMFQVFVLILTMLPLHAAAGFSFDDVVQKAKALAIKPYEAPQ
                     PIPKIMREIPYEAYQGIRFNPDRSLWKEKQSNFNVMFMMPGLYYTHPVTLNVIEAEGP
                     RPLPFKKTDFVFADPEVEKRVPADVGFAGFKLTFPLNNKNEQNQFVVFAGASYFRAVG
                     KDNVFGISGRGIAIDTGLSSGEEFPSFTEFWLVRPSPDAKEMIVYGLLDSKSLTGAYQ
                     FIIVPGSPTMMKVKTQLFPRKNIQLLGAAPLTSMFFYGENTPRPTGEWRREVHDSDGL
                     QIHAGAGGEWLWRPLLNPDTLEMDFFSTDQIQGFGLIQRDDKFWNYEDLGARYEKRPS
                     AWVEPRNDWGAGKVVLVQLPTPQETNDNIVAFWTPKT"
BASE COUNT          696 a          652 c          583 g          616 t
ORIGIN      
        1 gatcaaccca cggtccctgg atattcctcc tatcctcagg gtcaatatcc gcaacagagc
       61 aatgcctatc cgaataatca aaacgcctat cccaatcaga acccgatacc gaatcaggtg
      121 ccctattcga atcaaagtcc ctatccggaa aactcctacc cccaaagcca gccagagcaa
      181 tcgtattcca cgccgtatgg caattccgaa atagcacctt cccccgcttc tccgtatcca
      241 cccgaaggat cgcccgggta tcaatccact cagccggata ccacacagcc cgccatggtg
      301 ccgatcagcc tggatgtcct actggtcaaa aaacatctcg taaatggtgt ccaatcctac
      361 ctgcccatca aggacggaga ccttcttctg gatggacgcg ggaacgccaa ggccggggat
      421 aaattccgcg tcatgttccg cgcgaatacg gattgctatg tctatgtcat tgccgtggac
      481 ggatcggcct gggctcaggg gattttcccg acagcttcca gcccgttcgc caacccggtt
      541 aaagcgggac agcaacatgt cttgccgggt ggaaccaact ggttcagcct ggatcaatac
      601 aaaggaatcg agacgatttt tttcgtggcc tcacacgcac gccggccgga tatcgaagag
      661 attttgatga ccatctccgg acgcgagcga aacccgaagg aaaaacctca agccgtcaac
      721 gaacccgcta tcattccgaa tggctattcc ggtgtgcgcc cgggctcctc gccctttgca
      781 ttccaaacgt cacaacatca gcaacaggaa ttgctcccta ccacctattt cgtcaaaact
      841 gcaggggaag atctccgcat cacccgctgg ttccgccatc aataggtgcc tcaatataaa
      901 tggaatcttc ggagtagagc cttatggaca ccggaacaaa tagttcgaca atcctttaat
      961 aatctatgaa gaattgagta atttaatacc cctataagtt ttacattcga caaaattctt
     1021 gcaactgttc aaaacatatc caactactca tcaggtctct ctttgggata ctcatgaggg
     1081 ttttttaaaa aaaataaatg ggaagtgatg cattcgatcc gctttattcc gctttctctg
     1141 tcacaaatat gcgaaaatgt aatccggttt cattacattt caaaagatag atgggatgaa
     1201 cggcccaact cgattattga gaaatatccc gcctttccat caggtggatc ttttctcata
     1261 gagccgatgc acaagatagt caggccattt ctgaggccca tgaataatgc agacagaatg
     1321 gggtcctcct ccctataatc aattttccga cggagcgaac ggatccatcg attccgaatc
     1381 ggtgcggtaa cggcaatttc tggaaatcaa tttttaaaat caggagtatc gggaatgctt
     1441 aggatgtttc aggttttcgt gttgatattg accatgcttc cgctgcacgc tgcagcaggt
     1501 ttttcattcg atgatgtcgt ccagaaagcc aaagctttag caattaaacc ctatgaagct
     1561 ccccagccga ttccaaagat tatgcgtgaa attccctatg aggcctatca ggggatcagg
     1621 ttcaatcccg accgcagcct atggaaagaa aaacaatcaa attttaacgt catgttcatg
     1681 atgccgggat tatattacac ccacccggtg accttgaatg taattgaggc ggaaggtcct
     1741 cgcccgcttc cctttaaaaa aaccgatttt gtctttgctg atccggaagt agaaaaacgg
     1801 gtgcctgcag atgtaggttt tgcaggattt aaactcactt ttcccttaaa taacaaaaac
     1861 gagcaaaatc agttcgtagt atttgcgggt gccagttatt tccgggcggt gggaaaagac
     1921 aatgtatttg gtatatcggg acgtgggatc gccatcgata cgggactgtc cagcggggaa
     1981 gaatttccct ccttcacgga attctggctg gtacggccat ctccggatgc caaggaaatg
     2041 atcgtctatg gattactgga tagcaaaagt ctcaccggag cgtatcagtt catcattgtt
     2101 ccgggcagcc ccacgatgat gaaggttaaa acccaactct ttcccagaaa gaacatacag
     2161 ttattggggg cagccccgct taccagtatg tttttttacg gtgagaatac gccccgcccg
     2221 accggagaat ggcgcagaga agtacatgat tcagacggat tgcaaattca tgccggtgcc
     2281 ggcggggaat ggctatggcg gcctttgttg aatcccgaca ccctggaaat ggattttttc
     2341 agcactgacc aaattcaagg attcggctta atacaacgcg atgataaatt ttggaattat
     2401 gaagatctgg gcgcccgtta tgaaaagcgg ccaagcgcat gggtggagcc acggaatgat
     2461 tggggagcgg gaaaggtggt gctcgtccaa ctcccaaccc cgcaagaaac caatgataac
     2521 atcgtggcct tttggacacc aaaaacg
//
LOCUS       DATIAL010000505         6178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1273064, whole genome shotgun sequence.
ACCESSION   DATIAL010000505 DATIAL010000000
VERSION     DATIAL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273064"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VLA60_15445"
     CDS             <1..626
                     /locus_tag="VLA60_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF10805.1"
                     /translation="PQPEWTTEHDLVETIKGDIGGYEVKGYLLTDPATKQALMIDTGY
                     NAKQMLASISRRDLTLIGVCLTHGHTDHAGGLEEILSRWPVPVYLGNGDFDLLPWRPP
                     DASIKIPADHQVIALGELTVECLATPGHTPGGFCYLLSLKGQTLCFVGDTLFSGSVGR
                     SNPFSLYPQHLQSVRHTLLHLPKDTALFPGHGPSTTVTEEQAHNPFA"
     gene            677..1816
                     /locus_tag="VLA60_15450"
     CDS             677..1816
                     /locus_tag="VLA60_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSF10806.1"
                     /translation="MDHESPEPSTPLDPSENKPTPPPSLLNPIAPTDPLPRTNPPAPP
                     PLIRPISTNDIGQDHTTQTSSRGMDHWQSLQPELLETGDEVDEFPPPTPSAFSQWGLP
                     ILLFGLTVFTTLWAGAFQVNTKPVSGAWDFLMRYPGSLVNGIPFAATLLGILVTHEFG
                     HYLLSRIHRVPASLPLFIPGPPHFIGTFGAVIRMRSAIMNRRALFDIGVAGPIAGFIA
                     AVVAIIIGLSLSHIVPRSQTYGLQLGEPLLLQWFGWLMFGPIPPTHDIVLHPIAFAAW
                     FGFFVTAINLLPLGQLDGGHVAFAVLGRRQRSLALGTIPILIYLGLTGWPGWILWVGL
                     ASLIGIAHPPVIDPDVVLGKHRQWVAWAALAIFIVTFAPVPFSVG"
     gene            1931..3334
                     /locus_tag="VLA60_15455"
     CDS             1931..3334
                     /locus_tag="VLA60_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSF10807.1"
                     /translation="MSDYQSQLQTYIQDHRASFEDLLGQMVEVPSVSSDPEHAPDMHR
                     MAQLAVQYLEQFGAKASIVNTSGFPSVSGEWVAGRQYPSLTIYNHLDVQPAQEPEWRQ
                     PPFSFHKDGDRYCGRGATDDKGPALTALFAAKFAREAGIPLNIRVLWELEEEIASPNF
                     SEVLQNRKAVPASDSVLVSDTIWVAKDRPALSCGLRGLLVAKLVLRTGETDIHSGLTG
                     GGARNPLAELCEAAYSCLDAKTGRVKIPGFYDDVVPPTRTELKNFLASGFQVRLFKEA
                     YQLRSLRTSDPQDLLKRIWASPTFEVHGLTGGHMGPGVKTIVPAWGELKVSMRLVPNQ
                     HPSKIFRLLKKHLRTRNPDIEVINDGELEPYRGISEGPYAEALQEAVAQAFGRRPVFV
                     REGGSIGAIAVLQRAWKVPILFMGLSLPEHGYHAPNEYFDWRQASGGIQAFVHYFDHL
                     SRMRLEKPKARSKLITS"
     gene            complement(3370..3741)
                     /locus_tag="VLA60_15460"
     CDS             complement(3370..3741)
                     /locus_tag="VLA60_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10808.1"
                     /translation="MNRLLWTGVLILLNGLIFSGLGASYKLGSVGVQPGEEQGRDLYR
                     IHCLECHGAEGRGDGPRAALLAPRPGNLVSAATSAKTDAELLEIIAEGVPRTAMSGWS
                     HQLSVDERRNILAFIRTLVHF"
     gene            complement(3835..5253)
                     /gene="der"
                     /locus_tag="VLA60_15465"
     CDS             complement(3835..5253)
                     /gene="der"
                     /locus_tag="VLA60_15465"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HSF10809.1"
                     /translation="MARSSHSRTTRSRNRPSLESSLCPSDVPVEVPLEPSPYRPLVAL
                     VGRPNVGKSTLFNRIVGHRTAIVDDVSGVTRDRNTAECDYQNRIFTLVDTGGLDLTTD
                     DVIVEQIKFQTQAAIEEADLVIAVMDGRVGFSPLDADLVKLLRPVTKPVFLAVNKIDT
                     PQAEPLLADFYKLGIKTIFPISAEGGLGVDELLEALLPYLPQAPENEVPKEIPRIAVV
                     GRPNVGKSTLVNAILGEQRLIVSDIPGTTRDPIDSMVSLHGRRYIFTDTAGLRRRGRI
                     ERGIEGYSVVRAIRALGRSDVAVLVLDGVEGVTEQDTKIAGLISKQGRGCIILVNKWD
                     LREGDREAYPAYNLELARRFPFFTHVPVVFGAAIQPETLTRLFPKIDQVVADFSKRIQ
                     TRKLNLYVQELLEKNPMTLVRGNPKKSVFITQVATKPPTFALFVKSAPKVPASYLRYL
                     ENSIRTEYGFEGIPLRMLLRSQ"
     gene            complement(5681..>6178)
                     /locus_tag="VLA60_15470"
     CDS             complement(5681..>6178)
                     /locus_tag="VLA60_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10810.1"
                     /translation="LNQIIPILVHWLHLLSAVVWIGGLAFLVMAVTPSLKTAVPKQFI
                     RPISETFYRQYKRVVGILLVVILFTGGVNLHYVSEGMVMQSGENVAHHAKYLTVFFIK
                     LALVLGILTIFLYTIIFKNEPTGGETSEELEEMAVEPVPFQRIALWMGVFIILCAAAM
                     KHLHV"
BASE COUNT         1514 a         1652 c         1484 g         1528 t
ORIGIN      
        1 agcctcaacc ggaatggacg actgaacacg atcttgttga gaccatcaaa ggcgacattg
       61 gcgggtatga ggtcaaaggc tatcttttaa ctgatccagc caccaaacag gccctcatga
      121 ttgatacagg gtacaatgcc aagcagatgc ttgcgagcat ctcccggcgt gatttgacct
      181 tgatcggggt ttgcctcacc catgggcaca cggaccatgc gggagggctg gaagagattc
      241 tgtctcgatg gccagtgccg gtctatctgg gtaatggaga tttcgatctg ctcccatggc
      301 gaccacctga cgcctccata aaaataccag ccgaccatca ggtgattgcc ctgggagagt
      361 tgacggttga atgtttggcc accccgggcc atacccctgg aggattttgt tacctgttat
      421 cgctcaaagg ccaaaccctg tgttttgtgg gtgatacgct cttttccggt tcggtggggc
      481 gttccaaccc tttttcactg tatccccagc atttgcaatc cgttcgccat accctgttac
      541 acttaccaaa agacaccgcc ttgtttcccg gccatggtcc ttccacaaca gtgactgagg
      601 aacaagcgca caatccattt gcctgatatt gcatgtcaga ccatttactg gcttggcatt
      661 taataaggac gtgttaatgg atcacgaatc gcctgaacct tcaactcctt tggatccttc
      721 agagaataag ccaactcccc caccttctct gttgaatcct attgccccca ccgatccact
      781 gccccggacc aacccacctg ctcctccgcc tctaatcagg cctatctcca caaacgacat
      841 aggtcaagac cacacaaccc aaacatcctc ccgcggaatg gaccattggc aatcccttca
      901 acctgaacta ctcgaaactg gagatgaggt ggacgaattc cctcctccaa ctccgagcgc
      961 cttttctcaa tggggacttc ctattttgct ttttgggctc accgtattca ccacactctg
     1021 ggcaggagca tttcaagtga atacgaaacc cgtctctggg gcctgggatt ttttaatgcg
     1081 ctatcctggc tctttagtca atgggatccc ctttgccgca actcttttag gtatcctcgt
     1141 cacccatgaa ttcgggcatt acctcctctc tcgcattcat cgtgtgcccg cgtccttacc
     1201 gctgttcatc cctggccctc cacacttcat cggtaccttt ggggctgtga ttcgcatgcg
     1261 atcggccatt atgaatcggc gcgcgctttt tgatattggc gttgcgggtc ccatagccgg
     1321 ttttatcgca gcagtcgtgg ctattattat cggactctcc ctttcccata tcgtcccacg
     1381 atcacaaacg tacggcttac agttaggcga accactcctg ctgcaatggt ttggctggct
     1441 catgtttggt ccgattcctc ctacgcacga tattgtactc catcccattg ccttcgccgc
     1501 gtggttcgga tttttcgtca ccgcaattaa tttactgcct ttgggtcaat tggacggagg
     1561 ccatgtggca tttgcggtgt taggccgtcg gcaacgctca ctggccttgg gaacgattcc
     1621 catccttatt tacttgggcc tcacgggttg gcctggatgg attctttggg tcggcctggc
     1681 tagccttatc ggcattgccc accccccggt cattgaccct gacgtcgttt tgggcaaaca
     1741 ccgccaatgg gtggcttggg cagccttggc tattttcatt gtgactttcg ccccagtgcc
     1801 gttttccgtt ggctgacaga catgttagcc ttccctatgc cctggttgtt cacggactat
     1861 catccgtttt ctccaccatc gtttccgtat tggaattccc agcccaacca cgaaaaggaa
     1921 tcgtgatctc atgagtgact atcaatccca attacaaacc tacattcaag atcaccgcgc
     1981 cagctttgaa gacctccttg gtcaaatggt ggaagtgcca tcagtgagtt cagaccctga
     2041 acatgccccc gatatgcacc ggatggcgca gttagccgtt cagtatctgg aacaatttgg
     2101 agccaaggcc tctattgtga acaccagtgg tttccctagc gtgtccggag aatgggttgc
     2161 cgggcgccaa taccccagcc tgaccattta caatcattta gacgtccagc ctgctcagga
     2221 accggaatgg cggcagcctc ccttttcctt tcataaagat ggggatcggt actgtggacg
     2281 tggagcgacg gacgataaag gcccggcgtt aaccgccctt tttgctgcaa aatttgccag
     2341 agaagccggg atccccctga acatccgggt cctttgggaa ttggaagaag aaattgccag
     2401 cccgaatttt tcagaggtcc ttcagaaccg taaagcggtc cctgcgtccg attcggttct
     2461 ggtgtctgat acgatttggg tagcgaagga tcgcccggct ctctcctgcg gacttcgcgg
     2521 tttgttagtc gccaagttgg tgcttcggac aggggaaacc gatattcatt ccggtctcac
     2581 gggtggcggg gcgcgcaacc ccctggcgga attatgtgaa gccgcctatt cctgcttgga
     2641 cgcgaaaacg ggccgcgtga aaataccggg gttctacgat gacgtcgtcc cgcccacccg
     2701 aaccgaactg aaaaattttc tggcatcagg atttcaggtt cggcttttta aagaagccta
     2761 tcaattgcga tcgctgcgaa catccgatcc tcaggattta ctcaaacgaa tctgggcctc
     2821 tcccaccttt gaagtacatg ggttaacggg tgggcatatg ggcccaggcg tcaaaaccat
     2881 tgtaccggcc tggggtgaac tcaaagtgag tatgcgatta gttcccaacc aacatccctc
     2941 aaaaattttc cgactcctga aaaaacacct ccgtactcgc aatcctgata ttgaggtgat
     3001 taatgatgga gaactggaac cctatcgggg aatttccgag ggcccttatg cggaggcatt
     3061 acaagaggcg gtagcccagg cctttggacg ccgcccggtg tttgtacggg aaggtggatc
     3121 tatcggagcc attgcggtgt tacaacgtgc ctggaaggtc ccgattctgt ttatgggtct
     3181 cagtctgcct gaacatgggt atcatgcacc caatgaatat ttcgattgga gacaagcttc
     3241 aggggggata caagcctttg tccactactt cgatcacttg agtcggatgc gattagaaaa
     3301 acctaaagcc agatccaagc taataacaag ctaaggtcaa gaagaaggta cctgagctga
     3361 aggtaggtct taaaaatgca cgagggttcg gataaaggcg agaatattgc gccgctcatc
     3421 aacagacaat tgatgactcc agccactcat tgccgtccgg ggaactccct cagcaataat
     3481 ctcgaggagt tctgcgtcag ttttggctga ggtggctgcc gaaaccaaat ttcccgggcg
     3541 tggggctaac agggctgccc gtggaccatc cccccgtcct tccgccccat gacattccag
     3601 acaatgaata cgatagaggt ctcgcccctg ctcttctcct ggttgcaccc ccacactccc
     3661 taacttatag gaagcgccta aacctgaaaa gatcaggccg ttcagcagaa tcaggacacc
     3721 cgtccataag aggcgattca tgcctgtaat tgtattgcgt gaatggcgta tcttcaaaaa
     3781 ttcgcccatc aggcaatatt ttatacagga atcctgggaa gttgcaaaaa ctctttactg
     3841 actgcgcaac aacatcctga gcggaattcc ctcaaaacca tattccgtcc tgatggagtt
     3901 ttctagataa cggaggtagg aggctgggac ttttggtgcg gatttgacaa atagcgcaaa
     3961 ggtaggcggt ttggtcgcca cctgagtgat aaatacggat tttttcggat tgcctctgac
     4021 caacgtcatg ggattttttt ccaggagttc ctgaacatag agattcagct tacgggtttg
     4081 aatgcgtttg gaaaaatcgg ccacaacctg atcaatttta ggaaaaagcc gcgtgagcgt
     4141 ttccggttga attgcggcac caaagacaac cggcacatgg gtaaaaaatg gaaatcgcct
     4201 ggctaattca agattgtagg cggggtacgc ctcacggtcc ccttcccgca ggtcccattt
     4261 attcacaaga atgatacaac ctcgcccttg cttactgatt aagccagcaa ttttcgtatc
     4321 ttgttctgtg actccttcta ccccatccaa aaccagcacg gcaacatcag atcgtcccaa
     4381 cgctcgaatg gctcgcacga cactatagcc ttcaatccca cgctcgatcc gcccccttcg
     4441 ccggagacct gccgtatctg taaagatata ccgtcgccca tgaagactga ccattgaatc
     4501 aatcggatcg cgggtcgttc cgggaatatc actgacaatg agtcgttgtt caccgaggat
     4561 ggcattaacc aacgtcgatt tccccacatt gggtcggcct accaccgcga tccttgggat
     4621 ctcttttggg acttcattct ccggggcttg gggaagatag ggcaaaaggg cttccagtag
     4681 ttcatccacc cctaatcctc cctcagccga aatagggaaa atcgttttta tccccaattt
     4741 gtagaaatcg gccaagagcg gttcagcctg aggagtatcg atcttgttga ccgccagaaa
     4801 aaccggcttc gtcaccggtc gcaacaactt aacgagatcg gcatccaacg gagaaaagcc
     4861 cacccggcca tccatgacag caatgaccaa atccgcttcc tcaatggccg cttgggtttg
     4921 aaatttgatc tgctccacaa tgacatcgtc ggtggtcaga tccagaccac cggtgtcgac
     4981 cagcgtgaat atccgatttt gataatcgca ctctgccgtg tttctatccc gagtgactcc
     5041 ggaaacatca tcgacaattg ccgtacgatg gccgacaatc cgattgaata gggtggattt
     5101 gcctacattc ggccgtccca caagggctac caggggtcta tatggggatg gttctaaggg
     5161 aacttcgaca ggcacatccg acggacatag tgacgattca agagatggtc tgttacggga
     5221 gcgagtggta cgagaatgag aagatcgagc cattggacag caaataacga attagggtaa
     5281 tccaactggt aggaaaatta ggtttcaggc gtcaggtcgt ctaccagatc aacaagacga
     5341 tttatcgaag gaactcgaac agtgatagcc tggataaagc cccccagtca actatcggtg
     5401 gataaagctt gatttagaca cagaatttag ctcagcagcc acaaggaaat ttatcgagaa
     5461 gaatcttttc tgcagttcga ttggtttaag ggaaatggat ccatgcgaat tattcagcaa
     5521 tcgggtaaca actatgacgg gtatacgaag ggttagacat ttttcgtgat ccaccagcca
     5581 tttccactga tgcgctcaat tcatgaaatg attcagaaga ttgaagcagg gtaaaaggta
     5641 ggaaaactaa accaggcaaa attcgatgaa gaccaacgag ttacacatgg agatgtttca
     5701 tcgctgccgc gcaaagaatg ataaacacac ccatccacaa tgctatgcgt tgaaatggca
     5761 ccggctctac ggccatctct tccaattcct cgcttgtctc accgcctgtt ggttcatttt
     5821 taaagattat ggtgtacaga aaaatggtca gaattcccag caccaacgcc aatttgatga
     5881 aaaagactgt gaggtatttt gcatgatggg cgacgttttc tcccgactgc atgaccatcc
     5941 cctcactcac ataatggaga ttgactcctc cagtgaaaag gataaccacc aaaagaatcc
     6001 cgaccacccg tttatattgc cgatagaagg tttcagatat tggtcggata aattgtttcg
     6061 ggacagcggt tttaagactg ggggtcacag ccataacgag aaaggccagc cccccaatcc
     6121 acaccacggc agacaagagg tgcagccaat gcaccagaat gggaataatc tgattaag
//
LOCUS       DATIAL010000506         7500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_628375, whole genome shotgun sequence.
ACCESSION   DATIAL010000506 DATIAL010000000
VERSION     DATIAL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628375"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VLA60_15475"
     CDS             <1..696
                     /locus_tag="VLA60_15475"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10811.1"
                     /translation="EKGAFTGAHLQRPGRIESAQGGTLFLDEIGEISPALQVKLLRVL
                     QERSIERVGGRVEIPVDTRVLAATNMDLQLAMKDGRFREDLYYRLNTVTITAPPLRER
                     GGDIVMLGKSLLQRYAEEAKKKISGFTREALMSLEQYHWPGNIRELENRIRRAVTLTD
                     NPRIAPEDLDLSPPDESQDGLTLKVARDTVERRVIEQTLAKTGGNITKAATILGVSRP
                     TLHDLISRHHIKK"
     gene            complement(754..1962)
                     /gene="argJ"
                     /locus_tag="VLA60_15480"
     CDS             complement(754..1962)
                     /gene="argJ"
                     /locus_tag="VLA60_15480"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HSF10812.1"
                     /translation="MSKRIKGGITAPKGFLAAGIHSGIKKTKQLDLALIVSEKPGPIA
                     GVFTSNRLPAAPVTLDRLNLKRGTGQAFIVNSGNANAFTGPEGLIHAREMGRRVADEL
                     TIPLHHVFVGSTGVIGVPLPMPAVRNGIPILISRLRKAGHQEAAKAIMTTDTFPKEIA
                     VQDRIGGKIVTIGGIAKGSGMIHPDMATMLAYVTTDAAIDKKTLQTTFRAVTNQTFNC
                     ISVDGETSTNDMALCLANGEAGNVPIKTGTTAHGKFEALLFQVCHHLAMEIVRDGEGA
                     TKIIEFQITQASSDRAAKQFANTLATSPLVKTAIFGTDPNWGRIVAALGRAGVPVKAE
                     KLLIGFNKIPIVKNGKGLGPQVERRIKQEMKKHYLKIFISLGMGKGSSKIWTTDLTYD
                     YVKINASYRS"
     gene            complement(2006..3082)
                     /gene="argC"
                     /locus_tag="VLA60_15485"
     CDS             complement(2006..3082)
                     /gene="argC"
                     /locus_tag="VLA60_15485"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HSF10813.1"
                     /translation="MDRKIRVAVIGASGYTGGELLRLLTLHPQITLTRVVASEKSEGR
                     TVASLLPHLTKIYDCSLSSLNTDSIAKEVDVVFLALPHTQSLQPVADFLSQGKHVIDL
                     SADYRLQDPMVYEQWYQTPHTFPGLLKNAVYGLPELNRAAIAKTQLVAVAGCYPTVAI
                     LQLAPFVAQDLIEHNSIIIDAKSGISGAGRTPALGTHFPESHEAIHAYKIGKHRHVPE
                     IEQELARLIPAPSSKRAFPSPSIIFTPHLIPINRGILSTAYAKLKPGIDQSHLDAAYK
                     SRYQDEYFIRLFPSSEGVNPKNLRGSNFCDLSCTYDPRTGYLITTGSLDNLVKGAAGQ
                     AIQCMNLMLGLPETLGLTAPGLFP"
     gene            complement(3283..3675)
                     /gene="rpsI"
                     /locus_tag="VLA60_15490"
     CDS             complement(3283..3675)
                     /gene="rpsI"
                     /locus_tag="VLA60_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HSF10814.1"
                     /translation="MVDTIQYATGKRKNAIARAWVEPGQGDILINARSVDSYFPRLTH
                     QIQIQTPFEVTKTGGQFNVKATLTGGGVSGQAGALRHAIAKALALYNPSLRDPLKKNG
                     LLTRDSRVKERKKYGQKGARARFQYSKR"
     gene            complement(3722..4150)
                     /gene="rplM"
                     /locus_tag="VLA60_15495"
     CDS             complement(3722..4150)
                     /gene="rplM"
                     /locus_tag="VLA60_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HSF10815.1"
                     /translation="MRSYQAKPLEVERQWFLVDAKGKTVGRLAAKVASILRGKHKPTF
                     TPNVDTGDHVVIINSSEIQFTSNKFRTKIYYHHTGYPGGIKAITAEHLHAKKPTEVLT
                     KAIRGMLPKCTLGKQMAKKLKIYPGAEHLHRAQSPTPLNL"
     gene            complement(4188..4658)
                     /locus_tag="VLA60_15500"
     CDS             complement(4188..4658)
                     /locus_tag="VLA60_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSF10816.1"
                     /translation="MTEINTLIPLKVHGVLVDPNTDTQIVVLRDEKNSEVLPIWVGTA
                     EGTSIRLALENVIPPRPMSHDLICSFASHLGFTLNKVVITEVKNNTYFSTLFLSKDDL
                     EKSIDSRPSDAIALALRCQCPIYVTPEVLERRGGEDLDTWLSKLDQKGLEQTDI"
     gene            complement(4720..5175)
                     /locus_tag="VLA60_15505"
     CDS             complement(4720..5175)
                     /locus_tag="VLA60_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HSF10817.1"
                     /translation="MLNQMNVRGLLFDPYNNAYIVILRDEQNSDMLPIWVGKAEASAI
                     SFALERIAPPRPMTHDLMKTVLDNMDAKVISTVITDLKDNTYFAKIHLLFGDSEFTVD
                     SRPSDAIAVALRTDAPIFASGEVLHKQNSEELERWLENLKPEDFGKSDV"
     gene            complement(5416..7137)
                     /locus_tag="VLA60_15510"
     CDS             complement(5416..7137)
                     /locus_tag="VLA60_15510"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HSF10818.1"
                     /translation="MRVSQSLIPTMRQDPGEAEVVSHRLMLRAGMIRKVAAGIYSYLP
                     LGLRVIRKIETIVREEMNRIGAQELLLPILSSAELWKETDRWDFYGKELFRLHDRHER
                     DFCLGPTHEEVVTDLFRREVSSYRQLPVTLYQIQTKFRDEIRPRFGIMRGREFIMKDA
                     YSFDQDVDGAKRTYQDMHNAYCRIFSRMGLEFRPVEADTGLIGGISSHEFMVLANTGE
                     ELIVFDPDTQYAANVERAEIAPPTIGEPEAPLPLERVSTPNAKSVEEVCALLNVPPSR
                     LVKTLLYQTGTDEITAVLIRGDHQANEIKIQRHLGIHELSLATPEMVVRFTKAPVGFV
                     GPIGLQKIRILADQAVAVLSNFIVGGNEPDVHLIHVNINRDFAIESIGDFRQAQAGDP
                     SPRGGSSLQTARGIEVGHIFMLGTKYSEKMGATYLDAQGQTLPAVMGCYGIGVSRAAA
                     AAIEQNHDDKGICWPMPIAPFQVHILSVTASENVRQASEQLYQDLSQNGIEVLLDDRE
                     ERGGVKFNDADLLGIPFHIILGDKGLAQNTIEIKDRKTGEKHHISREKACEWVTAAVR
                     AKLEFQT"
     gene            complement(7231..>7500)
                     /locus_tag="VLA60_15515"
     CDS             complement(7231..>7500)
                     /locus_tag="VLA60_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSF10819.1"
                     /translation="IASVSGEQAQQGLASVVWLIAILSINLGILNLLPIPILDGGHLF
                     FFACEGILGRPLGDRSREMAQQIGLVLLVFLMAYATWNDISRLLQ"
BASE COUNT         1879 a         1723 c         1884 g         2014 t
ORIGIN      
        1 gagaaagggg cgtttaccgg agctcatctc cagcgtccgg gtcgaattga gtcggcccag
       61 ggcgggacgc tttttctaga tgagattggt gagatttctc ctgcattgca agtcaagtta
      121 cttcgggttt tacaagaacg aagtatcgag cgggtcggcg gacgcgtaga aattccagtt
      181 gacacaaggg ttctggcagc caccaatatg gatcttcagc tggcaatgaa agatggccgc
      241 ttccgggaag atctgtatta tcgattgaat accgtgacca ttacggctcc tcccttacgg
      301 gagcggggtg gcgatatcgt gatgctaggc aaatcgctgc ttcagcgtta tgcagaagaa
      361 gcgaaaaaga agatttccgg atttacgagg gaagccctga tgtctctcga gcaatatcat
      421 tggcccggaa acattcgaga attagaaaac cggattcgtc gcgccgtgac gctaaccgac
      481 aatccaagaa ttgctccgga agatttggat ttatcccctc ccgatgagag tcaagatggg
      541 cttaccttga aagtagccag agatacggtc gaaaggcgag tgattgagca aaccctagcc
      601 aagacaggtg gaaatattac gaaggctgcg accatccttg gtgtgagccg cccgaccctg
      661 catgatttaa tttcccgcca ccacataaaa aaataactat ccctcgtcgg taatagacaa
      721 ttcccaactt ccaaattgaa cagagaaaaa gagttaggat cgataggatg cgttgatttt
      781 tacgtaatcg taggtcagat ccgtggtcca gatcttggaa gatcctttgc ccattcccag
      841 tgaaataaag atcttcaggt agtgcttctt catttcctgt ttaattcggc gttccacctg
      901 aggccccagc cctttcccgt ttttgacgat gggaatctta ttgaatccaa ttagaagttt
      961 ctccgcttta acaggaacac cggctcgtcc taatgcggcc acaattctcc cccagttggg
     1021 atccgtccca aatatagcgg ttttgactaa aggggaggtg gctagggtat tggcaaattg
     1081 tttggcggcc cggtcactgg aagcttgagt gatctggaat tcaatgattt tggtggcacc
     1141 ttcgccatcc cgaacaattt ccatggccag atggtgacag acctgaaata ggagggcttc
     1201 aaattttcca tgtgccgtcg ttccggtttt aatgggtacg tttccagctt ccccgttggc
     1261 cagacataag gccatgtcat tggtgctggt ttccccatct acagaaatac agttaaatgt
     1321 ttggttggtg acagcgcgaa aggtagtttg tagcgttttt ttgtcaatgg ctgcatctgt
     1381 ggtgacatag gctaacatgg tcgccatgtc agggtggatc attcctgaac ctttggcgat
     1441 tccccctatt gtcactattt ttccgccaat tcggtcttga accgcgattt ccttaggaaa
     1501 ggtgtctgtc gtcatgatcg ccttggcagc ttcctggtgt cccgccttgc gtagacgtga
     1561 aatcaaaatg ggaatgccgt ttcgtaccgc cggcatcggt aggggaaccc caatgacacc
     1621 agtggatccg acaaacacat ggtgaagggg aattgtgagt tcgtcggcaa ctctccgacc
     1681 catctccctt gcatgaatta atccttccgg tccggtaaat gcgttagcat tcccactatt
     1741 aacaataaat gcttgccctg tgcctctctt aagattaagc ctgtcgagag tcacgggagc
     1801 ggccgggagc ctgttggagg tgaacacccc ggcgataggt ccaggcttct ctgacacgat
     1861 caaggccaga tcaagctgct tggtcttttt aattcctgaa tgaatccctg cggctaggaa
     1921 tcctttggga gcagtgatcc cgccttttat gcgtttagac atgagaacat ccccttttcg
     1981 tcaggagaaa ctggtgaaca atgttttagg gaaagaggcc tggcgcggtc aatccaagtg
     2041 tttcgggaag tcctaacata agattcatac attgaatcgc ttgcccggct gcacctttta
     2101 cgagattgtc cagcgaacct gtggtgatga gataccctgt tcgaggatca taggtgcaac
     2161 tgaggtcaca aaaatttgat cctcgcagat ttttcggatt tactccctcg gaactgggaa
     2221 agagtcggat gaaatattcg tcctgatacc gacttttata ggcggcatcc aaatgagatt
     2281 gatcaatgcc aggtttcaat ttggcatagg cggtgctcag aatgcctcgg ttaatcggta
     2341 ttaagtgagg cgtgaaaatt atagatggac tggggaaagc tctttttgaa gatggagcag
     2401 gaatcagtcg tgctaactcc tgttcgattt caggcacatg gcgatgtttg ccgattttat
     2461 aggcatgaat ggcttcatgc gattcaggaa agtgagtgcc cagtgcaggt gtgcgaccgg
     2521 ctcctgaaat tcccgacttt gcgtcaatga taatcgagtt gtgctcaatg aggtcttggg
     2581 ccacaaaggg cgcgagttgc aaaatggcga cggtgggata acacccggca acggcgacaa
     2641 gttgggtctt ggcgatagcc gcgcgattga gttctggtag cccatacacc gcatttttga
     2701 gcaggccagg gaaagtatgg ggggtttgat accattgctc atataccatc ggatcttgaa
     2761 gacgataatc ggcactcaag tcaataacat gtttcccctg tgagagaaaa tccgctactg
     2821 gctgcaagga ttgcgtatga ggaagcgcga gaaaaaccac atcgacttcc ttggcgatcg
     2881 agtcggtatt caacgaagac aatgaacagt catatatctt cgtaagatga ggaagcagcg
     2941 aagcgacagt gcggccctcg gatttttcag atgccaccac acgggtcagt gttatctgag
     3001 ggtggagggt gagtaatcga agtaattccc cacccgtata gccactggct ccgattacag
     3061 cgacgcgaat ttttctatcc atggcctttt tttaagaaaa ttgattgagg gggagaaagg
     3121 ttgagcagga tacctattag gaaatgattt tcctttgtga caattaatta tttgtgaaca
     3181 gttagcttgg agtggcaacg gcatcagaca gaagtcggat taaaaaaagg ggaggctgga
     3241 aagcctcccc cattaattct gcgtgaatgg tgaaccggcc aattatcgtt tggagtattg
     3301 gaacctggct ctggcgcctt tttgtccgta ttttttccgt tctttcaccc tgctatctct
     3361 ggtaagcaat ccatttttct tcaggggatc gcgtagcgaa ggattatata aggccaaggc
     3421 cttggcaatc gcatggcgaa gggctcccgc ctgacccgaa acgcctcccc cagttagtgt
     3481 ggctttgaca ttaaattgcc cgcctgtttt ggtgacctcg aaaggtgttt gtatctggat
     3541 ttgatgcgtc aatcgcggaa aatatgaatc gactgatcgt gcgttgatga gaatgtcgcc
     3601 ttgccctggt tctacccacg ctcgggcaat cgcatttttc ctttttccgg ttgcatattg
     3661 aatggtatcg accatgtgag aatacgtctc ctttttttcg ttggaacagt tactgatgaa
     3721 tttagagatt caaaggcgtt gggctttgcg ctcgatgcag atgctcggca ccagggtaaa
     3781 ttttgagctt tttggccatt tgtttcccca acgtacattt tggaagcatc ccacgaatgg
     3841 ctttggtaag gacttcggtt ggtttctttg catgaagatg ctcagcggta atggctttga
     3901 ttcctccagg gtaaccggtg tgatgataat agatctttgt acgaaattta ttgctggtga
     3961 attgaatttc actggaattt atgatgacga catgatcgcc tgtatcgaca ttcggggtaa
     4021 acgtcggctt atgctttcca cgaaggatag aggcaacctt ggcggccaag cgtccaacgg
     4081 ttttcccttt agcatcgact aagaaccatt gtcgttcaac ttccagcggt ttggcttgat
     4141 aagatcgcat gagtgtctga gttcctttcc tctacttgat tacatgtcta gatgtcggtt
     4201 tgttctaatc ctttttgatc gagcttggaa agccaggtat ccagatcttc ccccccgcga
     4261 cgttccagca cctccggagt aacatagatg ggacattgac atcggagagc tagggcaatc
     4321 gcatcactcg gccttgaatc gatggatttt tccaagtcat ctttggacaa aaacagcgtt
     4381 gaaaaatacg tattattttt gacttccgtg attacgactt tgttgagggt aaatccaagg
     4441 tgcgaggcaa agctgcaaat aaggtcatgg ctcatggggc ggggtgggat tacgttctcc
     4501 aaggccaacc gaatcgatgt tccttcggcg gttccgaccc aaatgggaag aacctctgag
     4561 tttttttcat cccgtaagac cacgatttgg gtgtcggtat tcggatcgac caggacccca
     4621 tggaccttta acggaattaa tgtgtttatt tcagtcatca cccgcacact cactatcact
     4681 ataaatcgta aatatttcag atagccacat gagcatcagc taaacgtctg atttcccaaa
     4741 atcctcaggc ttgaggtttt ccagccatcg ttccaattct tctgagtttt gtttatggag
     4801 cacttccccg gaagcaaaaa taggagcatc cgtgcgaaga gccacggcaa tcgcatcact
     4861 tggccgagag tcaacggtga actcggaatc tccaaaaagg agatggattt tggcgaagta
     4921 cgtattgtct ttcagatccg taatgaccgt actgatgacc ttggcatcca tattatcaag
     4981 taccgttttc atgagatcat gggtcatcgg ccttggagga gcgatccgct ctaaggcaaa
     5041 actaatcgcg ctggcttcgg ccttcccaac ccagattggg agcatatcag aattttgttc
     5101 atctctcagg atgacaatat aggcattgtt gtaggggtca aataaaagac ccctcacgtt
     5161 catttgattc aacatacgct atgccagtca taaataaagg acgtgtattt tacttgaaaa
     5221 tgaaaccttg aaaaatagca ttcaataaca ggtattgtca attaaaataa gggcttgtct
     5281 gactccgtat gctctagatt gggatctcgg cattggtcgt tctcaaaaaa gaaagcattt
     5341 tggtttcaaa aaattatacc atgcatgagt gtgcatcggt aaccaacctg catttgatct
     5401 gtttccctta tattatcatg tctgaaattc gagttttgct ctgactgcgg cagttaccca
     5461 ctcacaggcc ttctcacggg aaatgtgatg cttttcgccg gttttacggt ctttgatttc
     5521 aatggtattc tgtgccagtc ccttatcccc aaggataata tggaaaggaa tgccgagcaa
     5581 atcggcatcg ttaaacttaa ctccacctcg ttcctcacgg tcgtcaagta atacctctat
     5641 cccgttttgg gaaaggtcct gataaagctg ctcggaagct tgacggacat tttctgatgc
     5701 ggtgaccgaa agaatgtgga cctgaaaagg ggcaatgggc atgggccaac agataccttt
     5761 gtcgtcatgg ttttgttcaa tggctgccgc cgctgctctg ctgacaccaa tgccatagca
     5821 tcccatcaca gcaggaaggg tctgaccttg agcatcaagg taggttgccc ccattttttc
     5881 actatatttc gtccctagca taaaaatatg ccccacttcg attcctctgg ctgtttggag
     5941 cgagctgcct ccacgtgggg aagggtcacc ggcctgggct tggcgaaagt ctcctatgct
     6001 ctcaatggca aaatcccggt taatattgac atgaatgagg tggacgtctg gttcatttcc
     6061 acccacaatg aaattgctca aaacggccac ggcctggtca gccagaattc gaattttctg
     6121 aaggccgatc gggccgacaa agccgactgg ggcctttgtg aagcggacga ccatctctgg
     6181 ggtcgccaaa gataattcat ggataccaag atgtcgttga attttaattt cgttcgcttg
     6241 atggtcccct cggatcagga ctgcggttat ctcatccgtg ccagtctgat aaagaagcgt
     6301 tttcaccagg cgggaaggag gcacgttgag taaggcgcat acctcttcaa ctgatttagc
     6361 attgggtgtg gagacccttt ctagcggtag tggggcctca ggctccccga tggtgggtgg
     6421 ggcaatttcg gcccgttcga cattggctgc atattgcgtg tccggatcaa aaacgatgag
     6481 ttcttctccg gtattggcta agaccataaa ttcgtgagaa gagataccac cgattaagcc
     6541 ggtatcggcc tccactggac gaaattcgag gcccatgcgg gaaaaaatgc ggcaataggc
     6601 gttgtgcatg tcctggtacg tacgtttggc tccgtccaca tcctgatcaa agctatacgc
     6661 atctttcatg ataaattcac gccctcgcat gatgccaaac cgggggcgaa tttcatcgcg
     6721 aaatttcgtt tggatttgat acagagtgac gggcaattga cgataagaac tgacctctcg
     6781 acggaataaa tcggtgacga cttcttcgtg agtggggccc aggcaaaagt cccgctcatg
     6841 ccgatcatgt aaccggaata gttcctttcc gtagaaatcc cagcgatctg tttctttcca
     6901 aagctcggcc gaggatagaa tcggtagtaa taattcttgc gctcctatcc ggttcatctc
     6961 ttcgcgaaca atggtctcaa ttttccgaat gaccctcaat cctagaggaa ggtaggaata
     7021 aatgccggcg gcgacttttc ggatcatacc cgcgcgaagc atgagccggt ggctgaccac
     7081 ttcggcttcc ccgggatcct gtcgcattgt tggaattaat gattgtgata cacgcatgaa
     7141 gacacctggt tatatggttg aggacagact gagtcgcagg gagaaatcac atggatagca
     7201 aaaggcaacg agacgttcaa ctaggtggaa ttattggagt aggcgactaa tgtcattcca
     7261 ggtcgcatag gccattagga agaccaataa gaccaatccg atttgttggg ccatttctct
     7321 cgaccggtct cctaacgggc gacctaaaat accttcgcag gcaaaaaaga agagatgccc
     7381 tccatcaaga atgggaatgg gtagcaaatt caaaattccc aaattaatac ttaaaatcgc
     7441 aataagccac acgacgctgg caaggccctg ttgggcttgt tcgcctgaca cactggcaat
//
LOCUS       DATIAL010000507         3808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1273168, whole genome shotgun sequence.
ACCESSION   DATIAL010000507 DATIAL010000000
VERSION     DATIAL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273168"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..998)
                     /locus_tag="VLA60_15520"
     CDS             complement(54..998)
                     /locus_tag="VLA60_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10820.1"
                     /translation="MKTLTSSVNGMVTTQEEFQECISADFPFFLERASGIIKRNLQDM
                     GQWANDTSHEKSLRRLSFDLVVWFIEYCPSQLPGHPTLLLDGFISEFLERFQESNVSL
                     TSNSILYRFIEGLLRRAVVSRDALICLFYHFYGMKPAEVGCILGLDQGETLGIYKVFE
                     RWRQKGWHQAVKDIGLTDRELYALTETQLSQPKVFQEDVGKILKTLTPFCRKSEPPNN
                     SCLDEEKWYEMFREGYGCEFRMWHLPLCQSCMNTIAKFRNAFWLNATIPSQLHLHISP
                     HVLKKETGRRPSVAGNISSSGCSHSQCPGDIQIALGSG"
     gene            complement(1603..1785)
                     /locus_tag="VLA60_15525"
     CDS             complement(1603..1785)
                     /locus_tag="VLA60_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10821.1"
                     /translation="MTRSMTRYNYNLQCWVVDHIIQRCTHPSQMFCRCRGREFHGLTE
                     QEAVGVMTEALNTRAS"
     gene            complement(2105..2995)
                     /locus_tag="VLA60_15530"
     CDS             complement(2105..2995)
                     /locus_tag="VLA60_15530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HSF10822.1"
                     /translation="MVASRVSRLVQNLPLLSSQKEGSHFHEPLSPENADLHEAIKGLH
                     AFGELVGVSQEFRQALALGEAVAPTGVSVLIMGETGTGKELFARYIHELSPRRHKPFG
                     IINCASLPSELVESELFGHERGAFTGAQQCRQGKFELADGGTLFLDEIGEMPIQAQAK
                     LLRVLQDGMVDRVGSTQSRGVNVRIIAATNKDLTKAKGEGRFRSDLYYRLYVFPITTP
                     PLRQRREDIPLLARYFMERYSIQFSRQYEDIEPASLERLVQHSWPGNIRELKNVIECA
                     VILSQTALLHIDETALNICH"
BASE COUNT          925 a          952 c          943 g          988 t
ORIGIN      
        1 ttcctgagat acgtaagcag tgcctttctc ttccatcctt tttttaactg cctttaccca
       61 ctacccaatg ctatttgaat gtctccaggg cactgggagt gactgcaccc cgaagaagaa
      121 atgtttcccg ccacggatgg ccggcgtccc gtttcttttt tcagtacatg aggggagata
      181 tgcagatgga gttgtgatgg tatggtggca ttgagccaaa atgcgtttct aaatttggca
      241 atcgtgttca tacaggactg gcataacggt aaatgccaca tccggaattc acatccatac
      301 ccttccctga acatttcata ccatttttcc tcatccaaac acgaattatt cggaggttca
      361 cttttacgac aaaatggcgt taacgtcttc agaattttcc cgacgtcctc ttggaagact
      421 ttaggttgac ttagttgcgt ctcggttaat gcgtacaatt cccggtcggt caatccgatg
      481 tctttaaccg cctgatgcca tcctttttgc ctccaccgtt cgaacacctt atagatcccc
      541 agcgtttcgc cttgatccaa tcctagaata caacccacct cggcgggttt catgccataa
      601 aaatgataga acagacagat gagggcatca cggcttacca cagcacgacg gagcaatcct
      661 tcgatgaatc ggtacaaaat ggaattggaa gtcagcgaaa cgtttgattc ctggaatctt
      721 tcaaggaatt ccgaaataaa gccgtccaac aacaaggttg gatgacctgg caattgagac
      781 gggcaatatt caataaacca gacaaccagg tcaaaggaca gtctgcgaag cgatttttca
      841 tgtgaggtgt cgttggccca ttgccccatg tcctgtaaat tccgtttgat aatgcccgat
      901 gcccgttcca ggaaaaacgg aaaatcggcc gaaatacatt cctggaattc ttcctgagtc
      961 gtcaccatac cattgactga agaagtaagc gttttcacgg ggttctcctt tcaggcgaat
     1021 atgaccatat ataaatccat tgagctcgga gcgtcttcac ctttaccggc ctgtttcgag
     1081 atcacagagg tgctgattca acgcacacgt ggatgcacga tgtttgaatt ttctggaatg
     1141 aaaatttgtt cggtgatacc tgtttcggtt tttgaacact ctggtatccc tcatcttcag
     1201 atgattggcc agtatggcgg gaaggctagg ccctgtctgt tcaatattgc acagattgca
     1261 agagtttaaa tgagtacgta accggggttc tttcggttcg gagaagcggt cccgccgcca
     1321 caaggggttc aatcaagcgc ccagtgagcc cggccagctt cctctttgaa cccaggctca
     1381 tgtcagaggg ctgaaaaaga tgaaaggttg ttagtcgggc tttaccgacc cgaatctgga
     1441 ttttccaatg atccattttg ttcgcttagt ggtcagcttg tgactcccaa ggaattattt
     1501 gatggtttgt gtctgtgtgg tttccatggg tctcatcctt tggtgtggtt aggtgatcca
     1561 acgttacttc cttaaataat ctctcttggt cgacaatgtg attcaggatg ccctcgtgtt
     1621 tagtgcctcg gtcatgactc ccacggcttc ttgttcggtg agtccatgaa attccctccc
     1681 gcggcatcga cagaacatct gagagggatg cgtgcagcgt tgaatgatgt ggtccaccac
     1741 ccagcattgc aaattgtaat tgtagcgtgt catggagcgt gtcatggagt tctcctggtt
     1801 agacgttcgg gtggattcat ctgtctgaaa catctgcggc caaaggaaag acatggaaag
     1861 gtattgggaa aggttaaaaa cagagcttcg ctcttgcacg acgcggtgag cgccaccgcg
     1921 gttacagatt ggggttccgg ccgcggtagg ccaggttttc ccagagatgg cgggtcccag
     1981 gttccatacc tggttatggt ggggcacatc caggcgctcg tcaatttgcc atttccctca
     2041 tcccgattaa atttgatgca tgaccggaat tttttagatt ctttggttgc cgacctgctt
     2101 ccccttaatg gcaaatgttc agggccgttt cgtcaatgtg caatagcgct gtctgtgaga
     2161 gaatcacggc acattcaatc acgttcttga gctcacggat atttcccggc caggagtgct
     2221 gaaccagccg ctccagcgat gcgggttcaa tgtcctcata ctggcgactg aattgtatgg
     2281 aatatctttc cataaaataa cgagccaaca atgggatatc ttctcgacgc tgacgaagcg
     2341 gaggcgtggt aatgggaaag acgtaaaggc gatagtacaa atccgatcga aaccgacctt
     2401 cccctttcgc cttagttaaa tccttattcg tggcagcaat gattcgcaca ttaacccctc
     2461 gcgattgggt acttccgacg cggtcgacca tcccatcctg cagaacgcgc aacaacttgg
     2521 cttgcgcctg aatgggcatt tccccgattt cgtccagaaa gagggttccc ccatctgcca
     2581 gttcaaactt tccttgccgg cattgttggg cccctgtaaa cgctcccctc tcatgaccaa
     2641 acaactcgct ttctactaat tccgaaggga gcgaggcgca attgattatg ccgaacggct
     2701 tatgtcgacg cgggcttagt tcgtgaatgt atcgagcgaa cagttccttg ccggttccgg
     2761 tctctcccat tattagcacg ctgaccccgg tcggtgcgac ggcttctccc agcgccaaag
     2821 cctgcctgaa ctcttgactg acacccacca attcgccaaa agcatgaagg ccttttatcg
     2881 cctcatggag gtctgcgttt tccggcgata gcggctcgtg aaaatgactg ccctcctttt
     2941 gcgaactcaa tagagggagg ttctgcacta aacgggagac tcgcgaagcc accatatcgc
     3001 ctctgttttg ctcggtgata ggaagaaaag gtttctaact gaagtaatca atatagcgag
     3061 gaggctgggc gatgtctggt caatattgct cagattttca taattcacgt gcggagagac
     3121 ctctcatttt tccggtagga gaaacggtcc ggtagccgca aggcggtcta ttagtcaagc
     3181 gacaagtgag tccggccgga ttgccgtatc gcaaacaggc tcagggagtg ttgaataata
     3241 ataatttcca aggacatatt gatccacagg caccttggat atcagtggcc tcggggcggt
     3301 tcaaataagt tcgtccagca aggccacagt catttttgcg cacagagcgt acttccagta
     3361 cgtgagtacg aaaaaatggc gacctgcctg cgcgaagccg ctccggcgaa ggcagggaac
     3421 gccgctgacg gcttttttca acagccccgc tcaggaacgc gcaagctcac gccttgggac
     3481 agaaccaaat ggctttgccg ttcccaactc aatcagggac tggacacaaa tcagacacaa
     3541 atgcggtcca aaggcttttg atgtacagga cagttccaaa tcagtcgagc cacacatacg
     3601 gcagtagacc agattcaggg gggcagggtt tgaaaacagt cgagcagatc ggcaggtcat
     3661 gacgtctcct cctcagctat ggatgggaga ggggtgaatt cgtgagtctt atttaaatct
     3721 tagcaaagga acagcatgag atctggtcaa aattgctcac tgttgcgaaa ggccacagtg
     3781 tcgtcgagta gcatcgtacc ccgatcct
//
LOCUS       DATIAL010000508        10935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_951018, whole genome shotgun sequence.
ACCESSION   DATIAL010000508 DATIAL010000000
VERSION     DATIAL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951018"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VLA60_15535"
     CDS             <1..226
                     /locus_tag="VLA60_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HSF10823.1"
                     /translation="NAIGVILTGMGKDGAAGMLEMHRAGAYNFAQDEASCVVFGMPKE
                     AIKAGGVDKILPLHDIPAAMLAHLKMLAPR"
     gene            566..1396
                     /locus_tag="VLA60_15540"
     CDS             566..1396
                     /locus_tag="VLA60_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HSF10824.1"
                     /translation="MVTEITFEDIAYIRNLVRAKAAMVLEQEKIYLVQSRLEPFAKQE
                     GFGSMADLVSHLRQTSYGPLHKRVVEAMTINETSFFRDLLPFQALKDRLLPEVIQTNR
                     DTKRVHIWCGASSSGQEPYSILLTILHHFPELTSWHIRLIATDFSQEMVRRGQEGRYG
                     PFEVHRGLSPPVLNTYFSRQGMEWQIRDDVRDRIEFHEMNLAGLWLALPPMDLVFMRN
                     VMMYFDMETRKDILRRIRGILAPHGYLILGATETTLAVDDHFERIPVNGTSVYRVKKD
                     "
     gene            1754..2125
                     /locus_tag="VLA60_15545"
     CDS             1754..2125
                     /locus_tag="VLA60_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF10825.1"
                     /translation="MANTMEEYMNVTQEKSENVVVVHLAGRFDFGSRKTFKDSLGEAM
                     KEELPIVLNFGEVSFIDSSALGILVIAHQTLKSKKIPFSLVNPQPYVRQVLDLANVAK
                     MIPIYQTIGEVPQMAIVSSKV"
     gene            2235..2441
                     /locus_tag="VLA60_15550"
     CDS             2235..2441
                     /locus_tag="VLA60_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10826.1"
                     /translation="MKPMATVFHHPRRWVAGIHPEKRRWILEKQCRECRRLSEGLGVR
                     RAIRLTFHASTIQGKAVFAERRHL"
     gene            2438..3706
                     /locus_tag="VLA60_15555"
     CDS             2438..3706
                     /locus_tag="VLA60_15555"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HSF10827.1"
                     /translation="MITRKRLACSQIRAGMYLVRILDSWWKSPFFLHRRLLSSHDVQQ
                     LLESGIHEVEIDTAKGLDIPTDAESFLHPGKLECHSESKTPFDQDESSRHESSSCSPE
                     RKDGLEDGHQERLIRLRGDTIAALEDMFEGVKTGQAISHVAMQETARAFVEKALTHPT
                     VLAEIMLIEHLEQFDPTLYSHVVDTALLSILVGLQLKWEVKQLEEIAVAALLHDVGYM
                     RLPLNLVQSRWGSIGIDYSLLQQHVDMGVALINKHSEFTEDIVHMVQEHHAYLDGSGY
                     STILGGKPVSDSGILLGLTDYVDELLAVGNAGGSFPVALAIRRVYQEAQKGKFPTRFV
                     EAMIRVLGVYPVGTVVQLSTGEDAVVVKQNPEMSVRPEVKIFRTCTGEILKNPEVRNL
                     GTHSELKYEVRITKVLDSADPSINLREICS"
     gene            3710..6763
                     /locus_tag="VLA60_15560"
     CDS             3710..6763
                     /locus_tag="VLA60_15560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10828.1"
                     /translation="MWCNPHLFQTLWDGLMVGVCLVDREGQLTHMNLAGSRLLGWGAA
                     CPTNVSCHDLLECLVPCEEDGTTVCPFSGLLLEKSMVWVPRTRLRRRQGTWCWVELKG
                     LVVDDEETSGFLLMFRDLSSEMKLTEETRRLASIPKENPFPVIEVDSAGQLLYANPAM
                     VRLMEEAHIGQDGFSTALPDRFLELAERCFSQKHLEMHYEVSVGERQYSWTFAPHADL
                     GLLRGYGMDITEPKRAAEELSAFADTLEAKNHELDQALMKAESATRAKAAFLAVMSHE
                     IRTPLNGVIGMAEVLLASSLNHEQQECVNIIRMSGERLLAIINDILDFSRLESGQLAL
                     ESIGFNLTSLFEEVVDLFSERAHRKGLDLAAYVDPDVPSQLFGDPHRLRQILCNYLSN
                     ALKFTMKGSVLLRGSLILPSSSECDDSLGGLNHLSGDPDDETRLWIHLSVQDTGLGMS
                     EEVQEKIFHVFTQADSSMSRKFGGSGLGLAICKQLAELMHGTVGVKSQPNQGSTFWCD
                     IPCQASRTAHSPSTEGLSVAGKEVWVIGPQESSGWVMAKLLHEVGVKVVQIDSIQQAI
                     TLHESVHETDCSVVGVILSDRLEKEAVLKWFERVRSSSLFQEFQVWGMKPFWYRKDDE
                     ENSVRFNGMITLPLHRKQFFHCLFGQPDHPGPSDSPRATEPHPVEIVQMGIEGLEQSF
                     LNKQAGPSVLVVEDNPVNQRVAAGMLGKLGCQVTIVDTGAQALTVLKESVVDCILMDW
                     ELPDMDGLVITQRIRELEHARGFVHPAAYWHRHHGSASPPVLHIPIVGMTAHVLSEHG
                     QQCLENGMDDCLFKPVHLRDLERMLRRWVGFPSKGMDSPSSKTHAPADWTGIHSVATR
                     ESGTGHQERTSLWYPADRERYKVSAALQALEGDEELLYSLFHIFNATAPDLINGMHQT
                     IHLENRQELQRLAHQMKGALSAVHAMHERKKAEELEFTAVSATFSHLDATVIELERMV
                     KQLICEFECLIALQGKKKDGRSSEPISFGKGGTQIDYGTKAETSW"
     gene            6866..7093
                     /locus_tag="VLA60_15565"
     CDS             6866..7093
                     /locus_tag="VLA60_15565"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF10829.1"
                     /translation="MKDLLTLSEVSTFLKVPKSTIYKLARERRLPGHKVGKHWRFVRE
                     EIEAWVQNAGGDSVMVGAGSQQGVRDRFSLN"
     gene            7609..8010
                     /locus_tag="VLA60_15570"
     CDS             7609..8010
                     /locus_tag="VLA60_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HSF10830.1"
                     /translation="MIDTGIKVLVVDDMSTMRRIVKNVLRQIGFSDIVEAENGQDALT
                     KLKAGGFGLVVSDWNMPVMQGIELLRAVRADAELKTLPFLMVTAEAQKENLIEAVQAG
                     VSNYVVKPFTAEVLQGKLEKIFANVQPAKTS"
     gene            8056..8697
                     /locus_tag="VLA60_15575"
     CDS             8056..8697
                     /locus_tag="VLA60_15575"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HSF10831.1"
                     /translation="MLVDRMIEADVPGDEEISEEGLDEKRSIKTEMEELAKYVEVITQ
                     TIREMESPVTSTSDQLPQATLHLHDLAKMTEDGTHKVLSLTEELERNREVIQHHLEQL
                     RANVAGRAVKDLDAILTLVKADEGRLLNIHVALSFQDLVAQRVAKLVTILNEIQHKLL
                     KLVVIFGIQQKKGGAGSSSEGRGYEMLRQLESSKTTALQQELVDTIMSEYGMS"
     gene            8780..10684
                     /locus_tag="VLA60_15580"
     CDS             8780..10684
                     /locus_tag="VLA60_15580"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HSF10832.1"
                     /translation="MNDEMKEILNDFLAESSEMLEALDQHFVKLETEPSNTELLNEIF
                     RCMHSMKGSAGFLGFTHLVEVAHQAESLLNKLRQGEMKVSPFIIDIILEAVDAVKMLQ
                     SDIRETGGDSHVETQGIVNKLALTMDSADDLMLSVSSPKKTGMDTTSYAPSPSPAEKT
                     PSSMAEPPDTISLSSPFEARPPEGTVGIEGVSEPAAVSEGPVGLSDVLHKMKEATTRT
                     VQAASAPGSKLSGGKEEDQTVRVETKRLDHVMNLVGELVLNRNRLMKLGNGLEEQDEN
                     NPALRELNMTLAQLNLVTSDLQLAVMKTRMVPIRKVFSRFPRLVRDLAGKLGKQVRLE
                     LVGEDTEVDKSVADELSDPLVHLVRNSMDHGLETADDRKQHGKIPEGYVRLSAQQEGN
                     SIVIRVEDNGRGLQVERIAEKALEKGLVAQSELDSMSPREIMNLIFLPGFSTADQVSD
                     VSGRGVGMDVVRTNISRMNGSLELDSDPGQGSRVTIKLPLTVAIIQALMVEVECATFA
                     IPLASVVEAVKVTKDEIKSINRQAVLNLRERILPLLDLGETFHIPRDRTDDECYVVVV
                     KIGEQQFGVVVNRLRAQEEVVIKSLGEFLANVKCVAGATITGDGKVVLILDMADLVRE
                     VQAATYTGMR"
BASE COUNT         2759 a         2292 c         3031 g         2853 t
ORIGIN      
        1 aaatgcgatt ggtgtgattc tgacgggcat gggaaaagat ggcgctgcag gaatgttgga
       61 aatgcaccgg gcaggagcct ataattttgc tcaagatgaa gctagctgtg ttgtttttgg
      121 gatgccaaaa gaagctatta aggctggagg agtcgacaaa attcttccgt tacacgacat
      181 tcctgcggcc atgctggctc atttgaaaat gctggccccg cgttaaaggg agcctcccgg
      241 gtagttaagg tctcgagaca tgaacacgat tgccttttta atggccctta tgattctcct
      301 gacatcgtga ggctgaactc ttatctttgt acaattcgcc aaggggaggt cgttgaggtc
      361 tgtccctata acttggcagc ccttatctta ttcctcattt ttggctttgt ggaatttcca
      421 tagaggacat gctgcctctg tgttgcacct ttcctgcagg tctggaagat gtgaatttcg
      481 atgtatgggc tttaatcgag gagcttactt tgaacgttgc gtattcattc gtttccttat
      541 tagcctcatt accggtagaa gcatagtggt cacagaaatt acttttgaag atattgcgta
      601 tatccggaat ctggtccggg caaaagcagc catggtttta gagcaggaga aaatctattt
      661 agtgcaatca cgccttgagc cgtttgccaa acaggaaggc tttggatcaa tggctgatct
      721 ggtttctcac ctccgacaga cttcctatgg cccattacac aagcgggttg tggaggccat
      781 gaccatcaac gaaacaagtt tttttcggga cctccttcca tttcaagcac taaaagaccg
      841 gctcctgccg gaagtgattc aaacaaatag ggataccaaa cgtgtgcata tttggtgcgg
      901 ggccagttcc agcggacagg aaccctattc aattctcttg acgattttgc accattttcc
      961 ggaactgacg tcctggcata tacgtttgat tgccactgat ttttcccagg aaatggtcag
     1021 acgtggtcag gaaggccgat atggaccatt cgaagtccat cggggtcttt ctccgcctgt
     1081 gctgaatacc tatttttcac ggcaagggat ggagtggcag attcgtgatg atgtgcggga
     1141 caggatcgag tttcatgaga tgaacttggc cggtctgtgg ctggctcttc cgccaatgga
     1201 tctggtgttc atgcggaatg tcatgatgta ttttgatatg gaaacccgga aggatattct
     1261 tcgacgcatt cgaggcattc tcgcccctca tgggtatttg attctcggag ctacggagac
     1321 gacgttggcc gtggatgatc attttgagcg aattccggtg aacggcacgt ccgtgtaccg
     1381 agtaaagaag gattagtgga gggtgaggtt tttttatggt ctgatacggt tcgtattgcg
     1441 gcagttgatg ggcgtaacct ttcaatacgt tacattgtta accctcgata gaatcaggat
     1501 cacattccac tgcctcagcg cgaacggctc tcttgttggt gattgatttg ctctgtgtgg
     1561 ttctgagaac aaatggtgag aggggaggaa agctatggcg gcatgatgcc aagccagcgc
     1621 caaccgcaac ttccttccct gttaggaagt aaatcaatct gacgctacag ccattatgca
     1681 ggagtgttta attctttgcg aatcctcacc gataaggtga gttggatgat cacaggattg
     1741 tcaaggaggt ttcttggcaa acaccatgga ggagtatatg aatgttacgc aagaaaagag
     1801 tgaaaatgtc gtggtcgtgc atctggcggg acggttcgac tttggctcaa gaaaaacctt
     1861 taaagacagc ttaggagagg caatgaaaga agagttgccg attgtcttaa attttggaga
     1921 ggtatcgttt atagatagtt ccgctttggg tattttggtg attgcccatc aaactcttaa
     1981 aagtaaaaaa atccccttta gtctggtgaa tccccaaccg tatgttcgcc aagttttgga
     2041 tttagccaat gtcgccaaaa tgattccaat ttatcagaca attggtgagg ttcctcaaat
     2101 ggcgattgtg tcatcaaagg tataatcagt ctgtcccttt ttccggcctg aattaacccc
     2161 ctcaccaatt taattgtgat atggatagtg aaacggaaga agtgagggtg agggggtaag
     2221 gataagggct aggcgtgaaa ccaatggcaa cggttttcca ccatccccgc cggtgggtag
     2281 cgggaatcca tccggaaaaa cgaagatgga ttcttgaaaa acaatgccgg gaatgccggc
     2341 gtctgtcgga aggcttaggg gtgaggcggg caattcggct gacctttcat gcttcaacca
     2401 ttcaaggaaa agccgttttt gcggagcgtc gtcatttatg atcactcgaa agcgtcttgc
     2461 ctgttcacag attcgtgcag gcatgtattt ggttcgaatt ctcgattcct ggtggaagtc
     2521 gccgttcttt cttcaccgcc ggcttctttc ttcccacgat gtgcagcaat tgctggagtc
     2581 cggcattcat gaagttgaaa ttgatactgc caaggggctt gatatcccga ccgatgcaga
     2641 atcctttctg cacccaggca agcttgagtg tcattcggag tcaaagacgc ctttcgatca
     2701 agatgaatct tccaggcatg agtcctcctc atgctctcca gagagaaaag atggactgga
     2761 agatggccat caagaacgat taatacggtt gcgcggggat acgattgcgg ccttggaaga
     2821 catgttcgaa ggggtcaaaa cgggtcaggc catttctcat gtcgccatgc aggagacggc
     2881 cagggccttt gtggagaaag ccttaaccca ccctaccgtg ctggccgaga tcatgctcat
     2941 tgagcatctt gaacagtttg accccaccct ctactcacat gttgttgata ccgccttgtt
     3001 gtcgattctc gttggcctcc aactcaagtg ggaggtgaag caacttgaag agatcgcggt
     3061 cgccgcgttg ttacatgatg tcggctatat gcggctgcct ctcaacctgg ttcagtcacg
     3121 atgggggagc attgggattg attattcctt gttgcagcaa catgtcgata tgggagtggc
     3181 gctgattaat aaacattctg aatttacaga ggacatcgtc catatggtgc aggagcatca
     3241 tgcctatctg gatggctcag ggtattcaac cattttaggg gggaaaccgg tctccgattc
     3301 tggaatttta ctgggactca ccgattatgt agatgagttg ctcgctgtgg ggaatgccgg
     3361 cggatcattt cctgtggcct tagcgattcg gcgagtctac caagaagcac aaaaggggaa
     3421 gttcccgacg cggtttgtcg aagccatgat tcgtgtgttg ggagtgtatc ctgtgggcac
     3481 ggttgttcaa ctttccacgg gtgaggatgc cgtggtggta aagcagaatc ctgagatgag
     3541 cgtgagaccg gaagtgaaaa ttttcaggac ctgcaccggc gagattctca agaatcccga
     3601 ggtgagaaat ttggggactc attcagaatt aaagtatgaa gtgaggatta cgaaagtgct
     3661 tgattctgca gatccttcta ttaaccttcg cgaaatttgt tcctagctca tgtggtgtaa
     3721 tcctcactta ttccagaccc tttgggatgg tttgatggtc ggagtatgtc tggttgatcg
     3781 ggagggccaa ctcacccata tgaatttagc cggctcccgg ctgttagggt ggggagccgc
     3841 ttgtcccacg aatgtttcgt gtcatgacct tttggaatgt ctggttccct gtgaggaaga
     3901 cggcacgacc gtctgcccgt tttcaggctt actgcttgaa aagtcgatgg tttgggttcc
     3961 gcggactcgt ttgcgaaggc ggcaggggac atggtgttgg gtggaattga agggccttgt
     4021 ggtggatgac gaggagactt caggattcct gttgatgttt cgagatctca gttcagagat
     4081 gaaattgacc gaagaaaccc gccgattagc gtccattcca aaggaaaatc cattccctgt
     4141 gattgaagtc gactcggcgg gacaactgct gtatgccaat cccgctatgg ttcgcctgat
     4201 ggaggaagcc catatcggcc aggacggttt ttccacggcc ttgccggatc ggtttttaga
     4261 gttggctgag cgttgttttt cccaaaaaca tctggagatg cattacgaag tgagtgttgg
     4321 ggagagacaa tattcgtgga cattcgctcc ccatgccgat ttgggtctac tccgtgggta
     4381 tggcatggat atcacggaac ccaagcgagc cgcggaggaa ttgtccgcct ttgcggatac
     4441 cttggaagcg aaaaatcatg aactggatca ggcgttaatg aaggctgaat ctgctacccg
     4501 tgccaaggca gcgttcctgg ctgtgatgag tcatgaaatt cgcactcctt taaacggagt
     4561 gattggtatg gcggaggtct tgcttgcttc ctctctcaat cacgagcaac aggaatgtgt
     4621 caacattatc cgtatgtccg gtgaaaggtt gctggccatc attaatgata ttttagattt
     4681 ttcaaggctt gagtctggtc aattggccct ggaaagcatt gggttcaatt tgacctcctt
     4741 atttgaagag gtcgttgatt tattttccga gcgggcccat cgaaagggtc tggatttggc
     4801 tgcctatgtc gacccggatg tgccctccca gctgtttggc gatcctcatc gtttgcgaca
     4861 aattctatgc aattatcttt caaatgccct gaaatttacg atgaagggat cggtcctgct
     4921 gcgtggttcc ctgattcttc catcgagttc cgagtgtgat gattccctgg gtgggcttaa
     4981 tcatctttcc ggtgatcctg acgacgagac tcggttgtgg attcacttgt cggtccaaga
     5041 cactggcctt ggtatgtcag aagaggttca ggaaaaaatt tttcacgtgt tcacccaggc
     5101 ggatagttcc atgagtcgaa aatttggggg gtccggtttg gggttggcga tttgcaagca
     5161 attagcggag ctgatgcacg ggacggtcgg ggtgaagagt caaccaaatc aaggatcgac
     5221 attttggtgt gacattccct gtcaagcctc acggacagcc cattccccat ccaccgaggg
     5281 cttatcggtt gcaggcaagg aggtctgggt gatcggtccc caggaatcgt cagggtgggt
     5341 gatggcgaaa cttcttcatg aagtgggtgt caaggtcgtg cagatcgaca gcattcaaca
     5401 agcgatcacc ttgcacgaaa gtgtccacga aacggactgt tccgtcgtgg gggttattct
     5461 gagtgatcgt ctggagaaag aggctgttct gaaatggttt gaacgagtgc gatcctcgtc
     5521 cctgtttcag gagtttcaag tttggggtat gaagccattt tggtatcgca aggatgatga
     5581 agaaaacagt gtgagattta acgggatgat cacgcttccc ctacaccgaa agcaattttt
     5641 tcactgccta ttcggtcaac cggaccatcc cggaccgtca gattcaccaa gagcaactga
     5701 gccacatccc gttgagatag tgcagatggg gattgagggt ctcgagcaat ccttcctcaa
     5761 taagcaggcc gggccttcgg tgctggttgt ggaagataat cccgtgaatc aacgggttgc
     5821 agccggcatg ttaggaaaac ttggatgtca ggtgaccatc gtggacacgg gagcccaagc
     5881 tttgactgtg ttgaaagagt cagttgtgga ttgcattctg atggactggg agcttcccga
     5941 tatggatggg ttggtaatta cgcaacggat tcgggaattg gaacatgcca gaggatttgt
     6001 tcatccagct gcgtattggc atcgccatca tggttcggct tctcctccgg ttcttcatat
     6061 tccgattgtt gggatgacag ctcatgtcct ttccgagcat gggcaacaat gcctggagaa
     6121 tggcatggat gattgtcttt ttaagccggt tcatttacga gatcttgaac gaatgctccg
     6181 gcgatgggta gggtttcctt caaaaggcat ggactcgccc tcatcaaaga cacatgcccc
     6241 tgccgattgg acagggatcc attcggttgc caccagagag tccggaaccg gtcaccagga
     6301 gagaacttct ctctggtacc ctgccgacag agaacggtat aaggtctctg ccgcgttgca
     6361 agccctggag ggggatgaag aattgctgta ttccttgttc cacatattca atgccacagc
     6421 ccctgacctg attaacggaa tgcatcaaac cattcacttg gagaatcgtc aagagctcca
     6481 aaggctcgct catcaaatga aaggtgcgct gagtgcggtg cacgccatgc atgaaaggaa
     6541 gaaagcggaa gaacttgaat tcacggctgt ctcggcaaca ttttcacatc tggatgcaac
     6601 ggtgattgaa ttagagcgaa tggtcaagca gttgatctgt gaatttgagt gtctgattgc
     6661 tcttcaagga aaaaaaaagg atgggcggtc atctgaaccc atttctttcg ggaagggcgg
     6721 tacgcagatt gactatggca caaaggccga gacaagttgg tgaggagaac agaattttct
     6781 tctatccggg aagaagtctc aagtttccac atgatcagtc cgataccaca aacattacag
     6841 accaaactct aatgagggtg ccacaatgaa agatttgctg accttaagtg aagtgtcgac
     6901 gtttttgaaa gtcccgaaat cgacaatcta caagctcgcg agagaacgtc gactcccagg
     6961 gcataaagtt ggaaaacact ggcgtttcgt tcgggaggaa attgaagcct gggtacaaaa
     7021 tgccggagga gattcggtga tggttggtgc tgggtctcaa caaggggttc gggaccggtt
     7081 tagtttgaat tagcttcccg ctgagggcct ttgactttct cgaaagagga aggccgagag
     7141 ccactggaat gacctgctca tattctgagg gcaagatgcc ccggaatgat gagcgaggac
     7201 catttttttg atagacgtta aaaactttca actcattcaa tgtttagatc tcttctctcc
     7261 tcactaaaaa cctttctttc cctttccatc acttctgtct aattgtactt gtccctatgt
     7321 cgtcagttgt gtgggggata ttctgaatcc cggtgtcacg gattcgtaaa aaagcgtacc
     7381 gccgaaacgg tctttggaaa aggggtacat tcatttaaat aacgaacaag ggccgacctg
     7441 tggaattttc cagggacggc taaagatcgc agagaagtta accgaatttc gaatatgcca
     7501 cgttatgatc attacaatat gcaagtgtat cgttaaagaa taaatttcat tggtggaaaa
     7561 acccgaaatt gcctgggatt ctatgtccaa aggcgtaagg agcgcatcat gattgatacc
     7621 gggatcaaag tgttggtggt agacgatatg tctaccatgc gtcgaattgt taaaaacgtg
     7681 ttacgtcaaa tcggcttttc ggacatcgtg gaagctgaaa acgggcagga cgcattgacg
     7741 aaattgaagg ccggcggctt tgggttggtg gtgtcggatt ggaatatgcc tgtgatgcag
     7801 ggtattgagc tcttgcgggc ggttcgtgcc gatgcagaat tgaagacgct cccgtttttg
     7861 atggtcaccg ctgaagccca aaaggaaaac ttaatcgagg cagtccaagc cggggtcagc
     7921 aactatgtcg tcaagccgtt taccgcagaa gtcttgcaag gaaaattaga aaaaatattt
     7981 gccaatgttc agccggcaaa aacgtcataa ctgggaagga catcctccga atataacgat
     8041 ataaaggaga ttcttatgct cgtggatcgg atgattgagg ccgatgtgcc aggcgatgaa
     8101 gagatatccg aagagggctt ggatgagaag cgttcgatta aaaccgaaat ggaggagtta
     8161 gccaagtatg ttgaagtgat tacccaaaca atccgggaaa tggaatctcc tgtgacttcc
     8221 acctccgatc aactgcctca agccacatta catttacatg atcttgccaa gatgacggaa
     8281 gacggaacgc ataaagttct aagcttgaca gaagaactgg aacggaaccg agaggtgatt
     8341 caacatcatt tggagcaatt gcgggcaaat gtggcgggga gagcggttaa ggatcttgat
     8401 gcaattttaa ctttggtgaa ggcggatgag ggacgactgt taaatattca tgtggcgtta
     8461 tctttccagg atctcgtggc ccaacgcgtg gcaaagctcg tgacgatttt aaatgaaatc
     8521 caacataaat tgttaaagtt ggtggtcatt tttggaattc agcaaaagaa gggtggagcc
     8581 ggttcatcga gtgaaggacg ggggtatgaa atgctgcgtc agttggaatc atccaaaaca
     8641 accgccctgc aacaagaact ggtggatacc atcatgtctg aatacgggat gtcttaatga
     8701 ctatgcacga attctcagcg caacgtctca ggggggcgat gggctgaaaa aaaggcttgg
     8761 agctgagaac aggtgatcga tgaatgacga aatgaaagaa atactcaacg attttttggc
     8821 agaaagctcc gaaatgctgg aagctctaga ccaacatttc gtaaagttgg aaacggagcc
     8881 ttccaatacc gaactgctta acgagatttt ccggtgtatg cacagtatga aaggatccgc
     8941 cggttttttg gggttcaccc atttggttga agtggcgcat caagctgaga gtttgttgaa
     9001 caaactgcga cagggtgaga tgaaggtttc tccgttcatt atcgatatca ttttggaagc
     9061 ggtggatgcc gtgaaaatgc tccaatccga tattcgggag accggaggag attctcatgt
     9121 cgagacccag ggcattgtga ataaattggc gttaacgatg gatagcgccg acgatctgat
     9181 gctgtctgtc tcctctccaa aaaagacagg gatggacacg acctcttacg ctccttctcc
     9241 atctccggca gagaaaacgc cgtcttctat ggctgagccc cctgatacta tttccttgtc
     9301 ttctcctttt gaggcccggc cccctgaagg tacggtaggg attgaagggg tttcggaacc
     9361 agcggccgtt tctgaggggc ccgttggatt gtccgatgtg ttacataaaa tgaaagaagc
     9421 gacgacccga accgttcaag ccgctagtgc ccctggttcc aagctatcag ggggtaaaga
     9481 agaagaccag accgttcgag ttgaaaccaa acgcttggac catgtcatga atctggtggg
     9541 agaattggta ctgaatcgga atcgtcttat gaagctgggg aatggcctgg aagagcaaga
     9601 tgagaacaat cctgctctcc gggaattaaa tatgacgttg gcgcaactca atctggtgac
     9661 ctccgatttg caattggcgg tgatgaaaac acggatggtt ccaattcgca aggtgttttc
     9721 acgtttccca aggttggttc gggatttggc cggtaagtta ggtaagcaag tgcgattgga
     9781 actagtggga gaagataccg aagtcgacaa gtccgtggcc gacgaattaa gtgatccatt
     9841 ggtccatctg gtgagaaatt cgatggacca tggtttggaa acggcagacg accggaagca
     9901 acacgggaaa attccggaag ggtatgtgcg attgtcggcc cagcaggaag gtaacagtat
     9961 tgtgattcga gttgaagaca atgggcgggg gttacaagtt gagagaattg ctgaaaaagc
    10021 cctggagaaa gggctggtgg ctcaatccga actggattcc atgagtcctc gggaaatcat
    10081 gaatttaatt tttcttccag ggtttagtac ggcagaccag gtgagtgatg tgtcgggtcg
    10141 tggcgtgggc atggatgtgg ttcgaaccaa tattagccga atgaatggaa gtttggagtt
    10201 ggattctgat ccgggtcagg ggagtcgggt gacgatcaag cttccgttaa ccgtggccat
    10261 cattcaagcc ctaatggtag aggtggaatg tgccacattt gctatcccgc tggcttctgt
    10321 ggttgaggcc gtcaaagtca ccaaagatga aattaaaagt atcaatcgac aggcggtgtt
    10381 gaacctccgt gaacgcattc ttccgctctt ggaccttggt gaaacctttc acatccctag
    10441 ggaccggact gatgatgagt gctatgtggt agtcgtgaaa atcggtgaac aacagtttgg
    10501 agttgtggtg aatcgattgc gggcgcaaga ggaggttgtg attaagtcgt tgggggaatt
    10561 tttggccaac gtgaaatgcg tggcaggagc caccattacc ggagatggca aagtcgtgtt
    10621 gattttggat atggccgatt tagtgagaga agtgcaagcg gcaacgtata ccggtatgcg
    10681 ctaatccgtc aagcgtgagt tgggtctgag tgcccaggga attttcgtag acggaccggc
    10741 agcgatacaa agatgaaggt tggacgaatg ataccttctg tgctgagtgg aagactgagg
    10801 agaccgtaat ggcaaaatgg atggcagaaa ttgatcaacg gaccggacta gccggtgcca
    10861 ataagatgga actattaatg tttcattttg ggactaatga gacctatgga ataaatgtat
    10921 ttaagattcg cgaag
//
LOCUS       DATIAL010000509         6144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1057573, whole genome shotgun sequence.
ACCESSION   DATIAL010000509 DATIAL010000000
VERSION     DATIAL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057573"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1194)
                     /locus_tag="VLA60_15585"
     CDS             complement(85..1194)
                     /locus_tag="VLA60_15585"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HSF10833.1"
                     /translation="MTTKKGKSDLLPIKRRLRSNQTPAEQLLWSKLRGFQIHGFKFRR
                     QHGIGSYIVDFFCPERSLAIEVDGDVHANESKIKSDRQRENNLHSLGIKLIRYNNDDI
                     LHNLDGVLEDIALHVSKSSTAPNPSLQRRGRQKNDSISKFLLVANRRPRIFPTKEANP
                     EPYFPVTLQGLGNSPDPLPRKFFERPTLKVASELLGKVLIKQTPYGIIHSKIVDTEAY
                     VGPEDLACHASKGRTKRTEIMFGPAGFTYVYLIYGMYHCLNIVTEQEQYPAAVLIRGL
                     EILGGDYSPDWPRRIDGPGRVCRFLEVNRTHNGLDATLGSTLWIEDHGLAVLPKQIQT
                     LPRIGVAYAGEWAKKLWRFCLPASTPPKTRRVTPK"
     gene            complement(1457..3169)
                     /locus_tag="VLA60_15590"
     CDS             complement(1457..3169)
                     /locus_tag="VLA60_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HSF10834.1"
                     /translation="MSKQEPKTALDHVKQYHEQTKHEFNRYARSLGYLDWANQPDPFR
                     RFDGAPLVALPHLLLDEDPISPPYESLFHPPSIPSQPMTLNTLSRFFEYGLSLTAWKA
                     YNGTSWALRSNPSSGNLHPTEGYLFTGSLPHLALEPGLYHYAPKEHALEHRWALPPEP
                     AQSILQGIPSEGFLIGLTSVHWREAWKYGERAFRYCQHDTGHAIGTLRIAAATLGWNL
                     LVLSGTSDETIARLLGLTRPQDFQQAEQEYPELLAAVWRRNLQPSPTLNLAFNDLHDT
                     VLTSGEWKGTANRLSKDEPVLWEIIDSVTEASWKSTQEPEPIAFHASAAPMNEGKAIE
                     SEPLAGHIIHQRRSAVAFDGRTGLPADRFFTMLQRIMPRGGLPLPDRPMPWDAIPWEP
                     TIHLILFVHRIDGLRPGLYWVNRNPEHRDLMFFKNAMHEQFVWSTPEACPSNLPLYLL
                     EEGNAQNLAKQLSCGQDIAGDSAFALGMVAEFDKSLETYGPWFYKRLFWETGVIGQVL
                     YLEAEAAGIRSTGIGCFFDDPVHRVLGIHPQTKWQSLYHFTVGGPLDDGRLTTLPPYG
                     PHVT"
     gene            complement(3283..5226)
                     /locus_tag="VLA60_15595"
     CDS             complement(3283..5226)
                     /locus_tag="VLA60_15595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HSF10835.1"
                     /translation="MGKTYDASDIVVLEGIEPVRRRPAMYIGGTDKTGLHHLVWEILD
                     NAIDEVINGYATHIIVELDKSREGLRITDNGRGIPVDKHPKHKKSALEIIMTTLHAGG
                     KFDTGSYIHSGGLHGVGASVVNALSRHLEVQVKRDGKEWEQTYQAGVPQGPVKALGNA
                     RGTGTSVYFRPDPKIFGKTIQFDPAVLQDTLDAKAYLHKGLKLTFKDGTTGETHLFHH
                     EQGIQEYLRKLVADRGRKTTVDFVFYLERQLKNGASGPEGGAQALKMEVALQWTDEPA
                     EFVKSYVNGVATPNGGTHEMGLRGGIVKAARNYIDTHNLTPKGLSLQAEDIREGMAAV
                     LSVLVLHPQFQGQTKERLNNPEVQAQVDSLVRPALETYLNENQSIAETLVGRMILAAR
                     AREASREASKAVMRKSAISHRLNLPGKLADCQSTDPTISELFIVEGDSAGGTAKQGRD
                     LKNQAILPIRGKVLNTEELTLAKVVENKELADVVKVLGCGVGKDFDLKKLRYHKIILL
                     MDADIDGYHISTLLLTFFFRHMPDLIRQGHVYIAQPPLYRIEIGKEVHWAGTDEEKER
                     IVSGARSNANPTISRFKGLGEMFPQQLKVTTLHPATRRLLKVELLDERQTDRTFQDLM
                     GKRTEARYEFLMANAGLADNLDV"
     gene            complement(5498..>6144)
                     /locus_tag="VLA60_15600"
     CDS             complement(5498..>6144)
                     /locus_tag="VLA60_15600"
                     /inference="COORDINATES: protein motif:HMM:NF015922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HSF10836.1"
                     /translation="FGDPAQKLFKFKDGERIIEGLSFDPRMRTTGLSALAPSKGRNLP
                     LFDSTPSGLYGEAVAMTSSGMGIRFELDPFQEPSTRLGRKFCKLKEGEEVVGVSQVTA
                     PLGKTVVAVVSARGRALLCQAEEVAQLAGPGRGVIVLKLDQNDTVVGFRLLSSKEEEL
                     VLAKEDGGTLSVSLRKYQVVGRGGKGHPLLRRGRLTSMIPTELTVPIFQSEVTP"
BASE COUNT         1377 a         1552 c         1642 g         1573 t
ORIGIN      
        1 cgtagccaac atgtatctgg tgccctagcc taacgtagac cggctatctt gttcaagata
       61 tctggcaaaa ggattcgaaa gcatttactt cggggtgact cgacgtgttt ttgggggagt
      121 cgaagccggc agacagaacc gccacaattt ctttgcccat tctcccgcat aggccacacc
      181 aattcgaggc agcgtttgaa tctgttttgg caaaaccgct agaccgtgat cttcgatcca
      241 aagtgtgctg ccgagtgtgg catccagtcc attatgcgta cgattaactt ctaaaaatcg
      301 gcagacccgg cccggaccgt cgatgcgcct gggccaatcc ggcgaataat ctccccccaa
      361 gatttccagc cctcgaatta agactgcggc aggatattgc tcctgttcag tcacaatatt
      421 gagacagtgg tacattccat aaatcaggta cacataggtg aagccagcag ggccaaacat
      481 aatttctgta cgtttcgttc ggcctttgga ggcatggcat gccagatcct ccggtcccac
      541 gtaggcttcc gtatccacaa ttttggagtg gatgatcccg tagggcgtct gtttgatcag
      601 cacctttccc aacagttctg acgcaacttt caacgttggc cgctcaaaaa attttcgcgg
      661 taaaggatcc ggagagttac caagcccttg aagggtgact ggaaaatagg gttctggatt
      721 tgcttccttt gtaggaaata ttcgaggtct acggttcgcg actagcagga attttgaaat
      781 agagtcattt ttttgccttc ccctcctttg taaggagggg ttaggggcgg tagaactctt
      841 ggacacatgt aaggcaatat cttctaaaac cccatccaga ttgtgcagaa tgtcgtcatt
      901 gttgtatcga atgagcttga tccccaatga atgtaaatta ttctctcgtt gtcgatcgct
      961 cttgatttta ctttcgttcg cgtgcacatc gccgtcaact tcaatcgcca aggatctctc
     1021 cgggcaaaag aaatctacaa tgtatgatcc gattccatgt tggcgacgaa atttgaagcc
     1081 gtgaatttga aaaccacgaa gttttgacca aagcaattgc tcagccggag tttggtttga
     1141 tcgaagtcga cgttttattg gaagaaggtc tgatttccct tttttggtgg tcatgcgtca
     1201 ctgatcatat gtggtttgtt ttcccctctt ttgtaaggag gagagagggg aggtaaagac
     1261 tttcgccttc tcgttgagat ttcaaaaatt tgtgtgattt ctgatcggtt ggatgaactc
     1321 taccccacct acacctcccc ttacaaaggg gaggaaaaga aattactaca cataatggga
     1381 ttcaaggata ggatggtatc ccacctcttt aattcctctt cggaatgaaa ccccttacaa
     1441 aagggaggaa tatgcatcat gtgacgtgag ggccgtaggg tggcaacgtt gtgaggcgac
     1501 catcgtccag cgggccgcca acagtgaaat gataaagcga ttgccacttg gtttgaggat
     1561 gaatgccgag cacccgatgg accggatcat caaaaaaaca gccgatcccc gttgaccgga
     1621 ttccagccgc ttcggcctct agatagagca cttgtccgat cacccccgtt tcccaaaaca
     1681 accgtttgta aaaccacggc ccatacgttt ctaagctctt gtcgaattcg gctaccattc
     1741 caagcgcaaa tgcactgtcc cctgcaatgt cctgcccgca actgagttgt ttggccagat
     1801 tttgtgcgtt accttcttcc aagaggtaga gcggaagatt ggaggggcag gcttcaggag
     1861 tcgaccagac aaactgctcg tgcatcgcgt ttttgaaaaa catgaggtct cggtgttctg
     1921 gatttcgatt gacccaatac agaccaggcc ggaggccgtc tattcgatga acgaacaaga
     1981 tgaggtgaat cgtgggttcc cagggaatgg catcccaggg catgggtcga tccggtaaag
     2041 gcaagccccc tcttggcatg atccgttgca gcatggtgaa aaaacgatcg gccgggaggc
     2101 cggttcgtcc gtcaaatgcg acggcgcttc gccgttgatg gatgatgtga ccagccaagg
     2161 gctcggattc aatcgctttc ccttcgttca tgggggcagc tgaagcatga aaggcaatgg
     2221 gttcgggttc ttgagtcgat ttccaggacg cctctgtgac cgaatcgata atttcccaca
     2281 ggactggttc atctttgctc aaacggtttg ccgtgccttt ccattctccg gatgttagca
     2341 ccgtatcatg aagatcgttg aatgctagat tcagcgtggg agagggttga aggtttctgc
     2401 gccagactgc cgccagcaat tccgggtatt cctgttctgc ctgttggaaa tcctgcggtc
     2461 tggtcagacc caaaagccgg gcaatcgttt cgtcggaggt gccactcaac accaacagat
     2521 tccagcctaa ggtcgccgcg gcaattcgca atgtgccgat cgcatggccc gtatcatgtt
     2581 ggcagtatcg aaaggcccgc tctccatatt tccatgcttc acgccaatga acggacgtga
     2641 ggcctatcag aaatccttca gacggtatgc cttgaagtat cgattgggcg ggttcgggag
     2701 gaagagccca acggtgttca agggcatgtt ccttcggggc atagtggtac aggcctggtt
     2761 caagtgcaag gtgaggcaga gaacccgtga agagataacc ctcggtgggg tggagattgc
     2821 cgctggaagg attactccgc aatgcccagc tggttccgtt gtaggctttc cacgccgtga
     2881 gtgataaccc atattcaaag aagcgagaaa gggtgttgag ggtcatgggt tgagagggga
     2941 tcgagggcgg atgaaagagg gattcatacg gtggagaaat cgggtcctcg tcgagtagga
     3001 ggtgtggaag tgcaacaagc ggagcgccat cgaatcggcg gaagggatcg ggctgattgg
     3061 cccaatccaa gtatcctagc gaccgggcgt accggttgaa ctcatgcttg gtctgctcgt
     3121 gatattgttt gacgtggtca agggctgttt tcggttcctg cttggacatg tggctgttca
     3181 tatggacaag tgtaacaccg tgagaggtga gtgaaacaat acggcctgcc tcacctcgat
     3241 gagtcggcgt tcccaccctt tttattttcg gctgacgggt tgttacacgt cgaggttgtc
     3301 agccagcccg gcattggcca tcaaaaattc ataacgggcc tcggtgcgtt tgcccatcaa
     3361 atcctgaaag gtccggtcgg tttgccgttc gtctaacaat tcgactttga gtaagcgacg
     3421 agtggcgggg tgtaaggtgg tgactttgag ctgttgagga aacatttcgc ccaagccttt
     3481 gaagcggctg atggtgggat tggcattgct gcgggcccct gacacaatcc gttccttttc
     3541 ctcgtcggtg ccggcccagt ggacttcctt cccgatttca attcggtaga gtggtggttg
     3601 ggcaatgtag acatggcctt gccggatcag atcaggcatg tgccggaaaa aaaacgtcag
     3661 caacagtgtg ctgatgtgat agccgtcaat atccgcatcc atcaacagga taattttatg
     3721 ataccggagt ttcttgagat caaagtcttt cccgactcca cagcctaaga ctttgacgac
     3781 atccgccagc tctttgttct caaccacttt ggcgagggta agttcttccg tgttcagcac
     3841 tttgccccgt atgggaagaa tcgcctgatt ttttaagtca cggccttgtt tggctgttcc
     3901 gcctgcagag tcgccctcga cgataaacag ttcgctgatg gttggatcag tgctctggca
     3961 gtcggctagt tttcccggca gattgagacg atggctgatg gccgacttcc gcatcacggc
     4021 tttggaagct tcacgagagg cttcgcgggc tcgtgcggcc agaatcatgc gacccacgag
     4081 ggtttcggca atactttgat tttcgttgag ataggtttca agggccggcc ggaccagact
     4141 atccacttgt gcctgcactt ccggattatt gagccgttcc ttcgtttgcc cctgaaactg
     4201 tgggtggagg acgaggacgc tcagcacggc cgccatgccc tcgcgaatat cttcggcttg
     4261 aagggagagg cctttgggcg tgagattgtg ggtgtcgatg taattgcggg cagctttcac
     4321 gatgccgccc cgtaatccca tttcatgcgt gcccccattc ggagttgcca ccccgttgac
     4381 ataacttttg acgaattcgg cgggttcatc ggtccattgc aaggcgactt ccatcttcag
     4441 cgcctgtgcc cctccttcag ggcccgatgc gccatttttt aattggcgtt ccaggtaaaa
     4501 gacgaaatcg acggtcgttt ttcgaccgcg atccgccact aatttgcgca agtattcctg
     4561 gatgccctgt tcgtgatgaa acaggtgggt ttctccggtg gtaccatctt tgaaagtcag
     4621 cttgagcccc ttgtggagat aggccttagc atccagcgtg tcctgtaaca cagcgggatc
     4681 aaattgaatg gttttgccga atattttggg gtcgggacga aaatagaccg aagtcccggt
     4741 tccccgtgca ttgcccaagg ctttgacggg accttgaggt actccggcct gataggtttg
     4801 ttcccactcc ttgccgtcac gtttcacctg cacttccagg tgacgggaca gggcattcac
     4861 caccgaggcg ccgactccat gcaatccgcc ggaatggatg taacttccgg tatcaaactt
     4921 tcctccggca tgtaaggtcg tcataataat ttctaatgcc gactttttgt gtttgggatg
     4981 tttatccacg ggaatgccac gaccgttgtc cgtgatgcgg agcccttcac gcgatttatc
     5041 cagttccacg atgatgtgag tggcgtagcc attaatgact tcgtcaatgg cgttatccag
     5101 aatttcccag actaagtggt gcaggcccgt cttatcggtg cctccaatat acatcgcagg
     5161 acggcgtcgg acgggttcaa taccctccag taccacgatg tcactggcat catatgtttt
     5221 acccatgagt ggatccttgt gagtgaatgg gaatcaaaaa ggggagtgtt gaaaaatgcc
     5281 gccagcggcg ttctggcccc tttgccgggc tcacgtactc ctccgtacgc tccgcccatc
     5341 caagcggctg cggtcttgct ggacgcgcct ttttgaacac tccctcataa tcttcagatg
     5401 agtttcggtc attcatatgt ttatggaatg cccaaatagt tgaaatattc aacagccaca
     5461 aaagatcaac aagttcattg tatcgcgcgc caggaagcta gggtgtaact tctgattgga
     5521 agattggcac ggtcaattca gttggaatca tgctggtcag acgaccccgc ctaagcaatg
     5581 gatgtccttt ccctccacgt ccgaccacct ggtacttgcg aagggagacg gaaagggtgc
     5641 caccgtcttc tttggcaagc acgagctcct cctcttttga agacaataat ctaaatccaa
     5701 ccacggtatc gttctggtct aatttcaaaa cgatgactcc acggccgggt ccggccaatt
     5761 gtgccacctc ttcggcctga cagagcaggg ctcgtcctcg cgcggagacg accgccacga
     5821 cggtttttcc cagaggagcg gttacttggc tgaccccgac gacttcttcc ccttccttga
     5881 gtttacaaaa ttttcggccc agtcgggtcg agggctcctg gaaggggtca agctcaaatc
     5941 gaatccccat cccgcttgaa gtcatggcga cggcttctcc gtataaaccg gatggggtgc
     6001 tgtcgaagag tggcaggttg cgaccttttg acggtgcaag ggcggacaat cccgttgtcc
     6061 gcatacgcgg atcgaagctc agcccctcaa taatacgttc gccatctttg aatttaaaaa
     6121 gcttttgcgc cggatctcca aagc
//
LOCUS       DATIAL010000510        14076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_735740, whole genome shotgun sequence.
ACCESSION   DATIAL010000510 DATIAL010000000
VERSION     DATIAL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 14076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14076
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735740"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VLA60_15605"
     CDS             <1..562
                     /locus_tag="VLA60_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10837.1"
                     /translation="AGLVNDRSNEVETDVFGLSGSNDSNNGYYVGAGLDLVLTRDIWD
                     LFPGTWVLGEIGVEYKRFNSKNVTQAVPSTCALAGVPTCSVETNKKVQITMLTVNVAP
                     KIMFMEGSRFRPWVIPVGLDFHVISPPSNDTTVLDIGVQFAVGAQYRIWKAIHLGMDG
                     RFHLATGQTDTTNNFGTAGAYVGIAF"
     gene            959..1345
                     /locus_tag="VLA60_15610"
     CDS             959..1345
                     /locus_tag="VLA60_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10838.1"
                     /translation="MRQDSDPHTAQRGFISLARRFRTLVWSALFMLVVTGSVLLGNVI
                     NFRAPLSSWPPVVLAKLILVFLLAVVSFSHDRIMAQKVRTLKSKAASKLSAGEKLLLR
                     LSPLLGRLTLLLGLGVLLSAVIMVRS"
     gene            complement(1435..1779)
                     /locus_tag="VLA60_15615"
     CDS             complement(1435..1779)
                     /locus_tag="VLA60_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HSF10839.1"
                     /translation="MGSKLYVGGLPYSATQAELTDLFSAHGTVESANVISDKFTGQSR
                     GFGFVEMSTGEEAQAAIAALNSTEFGGRTLTVNEAKPQAPRTGGGGGGRGGFGGSGGG
                     GGDFGGKRRSRF"
     gene            complement(2087..3349)
                     /locus_tag="VLA60_15620"
     CDS             complement(2087..3349)
                     /locus_tag="VLA60_15620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF10840.1"
                     /translation="MWFKQIICLIMLYGFCWQSVLAATVPTPQAAAPSYSLQDILAIA
                     LEKNPLMNESEGVIEQKEGNKISASAYPNPTIALQSGRGTVRDPTGITLTERYVTLYQ
                     PIEWPGKRAARQQAAQAGLDGALASKEEAKLNLIAATKQAFYDLLFAERQTETAIQNV
                     QTMEEVRKAVIRRVEAGEAPPFDAVKIKVEGMKVQKEVARTRGVVQSIQASLNSLTAG
                     ALGLEFSIQGDFAVWPGALHLETLSEQTLANHPIITKWKKLIVQAQQTHRQEQQARVP
                     DVTLSGGYQRDAGREAYIAGVSIPFPVWNQRQGDIAEAKGTLRQREASLLRAKQELLK
                     GIAQQIQLSQAAAAQIATYEKGLLKQAQEALRIAQVSFKYGEASLLDVLDAQRVLRQT
                     QIDYAQAKYDLSIALTELERVTGGTVQP"
     gene            complement(3384..6500)
                     /locus_tag="VLA60_15625"
     CDS             complement(3384..6500)
                     /locus_tag="VLA60_15625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSF10841.1"
                     /translation="MERLLTLSLRYRFFTVVALLLVIASGVWSLTNLTIDAVPDLTPV
                     QVQILTRSPALGPIEVEQFITFPIETALSGIPGLQKVRSVSRYGLSAVTAIFEEHLNL
                     YFVRQLVNERLTLATERIPAAYGRPMMGPLSTGLGEVYQFTLTGQGHSPMFLRTLLEW
                     DIGRRLRAVPGVVEVNIWGGETKQFQVVVDPGKLLAFNLTLKTVFDALAQNNALAGGG
                     YIEHEREQYLIKGEAMASTITDLGQIVVSHGPGGVPIFIRDLGEVKEGAALRIGAATR
                     MGQGETVIGMVQMLAGANAQQVVKRVKERIQEIQATLPPGVVIEPYYDRTLFVSKVIN
                     TVRNNLLEGGLLVIAILFSFLGDIRAGCIVALAIPLSMLMAFTGMYHAGISGNLMSLG
                     AIDFGLLVDGSVVMIDNILRRLGNVTGQSKEEKLATIQEAAREVLRPIVFAVGIIIVV
                     YLPILSLIGLEGKMFRPMALTVIFALAASLLFAVGGVPVLSYWFARARPNHEETWLIR
                     RLRRLYRPWLAVSLNRPALVVIPGILLFILSLVIGSRLGIEFVPRLEEGDMAIQVWRL
                     PSVSLTESVETALGVERALRQFPEVVQVVTRTGSPEVATDVMGIDMSDVFVILKPRNE
                     WTTTTSKETLIENFKTAVLAAVPGVGLSFTQPIEMRFNELIAGTRSDLAIKIFGEDLE
                     VLRHQGDGVARALEQVPGAADVKVEQVTGVPRIRIIVDRDQIGRYGLSARDVLGIIES
                     ARVGQTVGTVFQGQRRFDLIVRLSDETSAGPIALGNILIPTAHGELVPLSRVATIEVD
                     EGPAQVSRENIQRRLVVEANIRGRDLGSFVTEAQQVIRDRVSLPPGYYLEWSGQFKHL
                     QEASRRLAVVVPITLLLIIAILSVIFGTIRPALLIFLNVPLALSGGIFALWMRGLPLS
                     ISAVIGCVALFGIAVLNGVVLVSRIQRLETEGLPTCEAVAQGAMDRLRPVLMTALVAS
                     LGFLPMAVATSMGAEVQRPLATVVIGGLITSTALTLFIIPALYGTVCRSNKQPAIPHQ
                     SHTNTTSI"
     gene            complement(6584..7720)
                     /locus_tag="VLA60_15630"
     CDS             complement(6584..7720)
                     /locus_tag="VLA60_15630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF10842.1"
                     /translation="MGCSDTQPPTGDLPDTSVTASSPDPSSSPGQPFHVDYVPEQARQ
                     RIRIEEVKERLTPHSVSAPGNVALDLAHVAKVSSRIPGQVDRVMVQLGDRVTKDQPLA
                     AVESMRLDELVQEYLVAKSKLDVASSNFKRTKQLLAENIISQRRFLEDRGQQIEAQAV
                     YQHVREKLLNMGLSQQELQQLEHGSHLEGHKYLLRAPLSGTVVSQQIVLGQGVTAGDE
                     FFEIVDTSRVWVFANLPIEQARRFREGDEGQVLPRGGEPITARLTYISPVADATTRTI
                     RVRFDVENPKGRLKPNEYVEVRLIDQQHPALSIPLSALTMLEGVRGVFVQRDNGYDFV
                     FVETGREGAGLVEIKRGLKLGEHVVTEGVFDLKNAIMKTSIQGE"
     gene            complement(7830..9272)
                     /locus_tag="VLA60_15635"
     CDS             complement(7830..9272)
                     /locus_tag="VLA60_15635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10843.1"
                     /translation="MQAFRTRIRRVALGFIVILLGLFSLLLYLGLSTILHRHIDGELM
                     ALAQEESLRVDLATDHVRVSPHRKHEEDELEEFDDEDELQQAIRYSAILDSAGRILWS
                     GQSTENRLPVTSQLIMQVLNGETVFETFHKPDHPPIRRISIPLVIEDHGRFILQTEKS
                     LQFVQETLQWLAWLLCGTSAISLLFALWGSGRLADEALSPVRALSQTAETVSGQTIST
                     RLTLSSPYAEFQRLAHTFNGMLDRLQQVFEGQRRFVADAAHELKTPLTAMKGNFEVSL
                     QRARSAEEYRETILSNLAEVDRLTAMTKSLLTLAQFAGDRPPLVLQSLDLPPLVKEIV
                     CELSVLAHEGGIHLQTELDSVPPLMGDASQLKQALINLLDNALRHTPSDGTITVRVGA
                     QDRSIRLSVEDTGPGIDSQHLPHLFGRFYRIDQARDRQSGGTGLGLAIVKEIIEAHGG
                     TIHAQSQIGKGTTFTITLPTGDEPPLRGAP"
     gene            complement(9276..9953)
                     /locus_tag="VLA60_15640"
     CDS             complement(9276..9953)
                     /locus_tag="VLA60_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF10844.1"
                     /translation="MRILLVEDDSSVSGFIVKGLREEQYAVDLATDGEMGLAMAETTR
                     YDVIILDIMLPKMNGIDVCRRLRAKRHTTPILLLTAREAIEDRVTGLDTGADDYLTKP
                     FAFAELLARLRALLRRGSAQPSPRLTIADLELDPVTHRVSRAGQTIILTNKEYSLLEY
                     LMRNTGRVLTRTAITEHVWDIHYESVTNIVDVHIKTLRSKMDRDFSPQLIHTVRGIGY
                     VLKIPEA"
     gene            complement(10151..10426)
                     /locus_tag="VLA60_15645"
     CDS             complement(10151..10426)
                     /locus_tag="VLA60_15645"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HSF10845.1"
                     /translation="MLVHFPIALLFTSVLFDAAGAWFKRENFRDGALWLLSLGLLGGV
                     AAAMAGDWAEEAAEKAGIAESMIETYETLAFVTLGIFGVLLLGRLVL"
     gene            complement(10530..10886)
                     /locus_tag="VLA60_15650"
     CDS             complement(10530..10886)
                     /locus_tag="VLA60_15650"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HSF10846.1"
                     /translation="MKRLHTISLLGTSALLLTSVGLAMSHDGKDKAAVANLATSVTME
                     EAIKTATTQFPGKVLEAELESEDGKVRYEVEIVNAAGEIREFEIDAQSGKILSSELEN
                     EEGHEKGASHKDTEKS"
     gene            complement(11139..12209)
                     /gene="ychF"
                     /locus_tag="VLA60_15655"
     CDS             complement(11139..12209)
                     /gene="ychF"
                     /locus_tag="VLA60_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HSF10847.1"
                     /translation="MEIGIVGLPNVGKSTIFNALTSGNAAASNYPFTTIEPNVGVVAV
                     PDARLGRLTELVKPKKTVPTSCRFVDIAGLVKGASQGEGLGNKFLANIREVDAILHMV
                     RLFQDPDVVHTMGGVDHRRDIEVIETELMLADLDSLTKQYDKVTAKAKSGDKASKEAL
                     VVLDILKSGLEAGKPARALGLEPEVLKNFFLLTTKPVLYVGNTDENPDPAILADFHAF
                     AKERGSESVVICGKLESEIAQLSGEDREIFMKDLGMEQSGLEKVVVAAYKTLGLCSFL
                     TSGVQEVRAWTIPIGAKAPQAAGVIHTDFEKGFIKADIYAFEDIDKYKSEAALREKGL
                     IRSEGKEYVVKDGDVCHFKFNV"
     gene            13079..13972
                     /locus_tag="VLA60_15660"
     CDS             13079..13972
                     /locus_tag="VLA60_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HSF10848.1"
                     /translation="MPEKSRRPSQGGAVMQEDKNQIVSGILRARAELEQVLYEMEKLP
                     AISESAVHFAAHALNNYLTVTGGTVELLLLMLANHPDKQVRTGLEALQQATNLMAHTV
                     SRLVNAESGLDAEMRFEKVELPIMAQRFRLFYQRIADQKQIQCLVGSAVDVPPVWTDR
                     VATAAALDNLFSNAVKYSPPGKRIWVEVLPQQDWVVCRVRDEGPGLSPEDQAKLFQRG
                     ILLTPRPTGGEPSTGYGLAVAKELIEKVGGEIWCESVLGQGACFSFRLPQYQELVHGS
                     DGMVPGSHEGRKHESRTVRHT"
BASE COUNT         3236 a         3622 c         3879 g         3339 t
ORIGIN      
        1 ggcagggtta gtgaatgatc gttcgaatga agtagagacc gatgtttttg gattgagtgg
       61 gtccaatgat agtaataacg gctattatgt tggcgctgga ctggatctgg ttctcacgcg
      121 agatatttgg gatttgtttc ctggaacctg ggtgttgggg gaaatcgggg tggaatataa
      181 acggtttaat tccaaaaacg tgacgcaggc ggttcccagc acctgtgccc tcgcaggagt
      241 cccgacttgc tcggtcgaaa ctaataaaaa ggtccaaatc acgatgttga cggtgaatgt
      301 ggcgcctaaa atcatgttta tggaaggcag ccgattccgt ccctgggtaa ttccggtagg
      361 gctggatttc catgtcatca gtccgccatc aaatgatacg accgtcctgg atatcggtgt
      421 ccagttcgcc gtcggggcgc agtatcgaat ttggaaagcc attcatcttg gaatggacgg
      481 ccgatttcat ctcgcgaccg gccaaacaga tacgacgaat aatttcggta ccgccggtgc
      541 gtatgtcggc atcgcgtttt aattatcgga aagggaatca tgggaagcgg cggagatgag
      601 attgtgatcg gacctggttg acctaagtgc aattggagag gaaaattctg ccgtctataa
      661 aagcagcagc aacgaggaca tcgttgcgtc cgtcataatc cggcttcaac tatagggaga
      721 cgctgttttc gagtattcgc ctgtcggaag tggtagctac gtatgataga ttttaccact
      781 cactcgaaat ggtcattttt tcgagagcgg atacattttt atctggtgag gacacagcaa
      841 tcccgctaca atccctgagg tgaatttttt ttacattatt ctggtctgga cccatctgat
      901 tgcagcaacc ttctggattg gagggatgct tttcctctcc cttgttgcag tgccactcat
      961 gagacaggat tccgaccctc ataccgctca acgcggattt ataagccttg ctcgtcgatt
     1021 cagaaccctc gtatggagcg cccttttcat gctggtggtg acggggtcgg tccttcttgg
     1081 aaatgtcatc aatttccggg ctcccctttc ctcctggcca cctgttgtcc ttgcgaaact
     1141 gatactggtg ttcctgttag cggtggtttc cttcagtcac gaccggataa tggcacaaaa
     1201 agtacgcacc ctgaagagca aggctgcttc aaaattatcg gctggcgaaa agctccttct
     1261 ccgattatcc cccctccttg gccgactgac cctgttgtta gggttgggag tgcttttgtc
     1321 tgccgttatc atggtgagat cctgagggta acgatctgaa ttccgggtat ggaataaaaa
     1381 aaagggagag gccttgtgga cctctccctt attatcagac gtatagagtg atcgttagaa
     1441 tcggctgcgc cgctttcctc caaaatctcc tccgcctccg ccactgccac cgaatccacc
     1501 acggccgcct ccgccgccac cggttcgtgg agcttggggt ttggcctcgt tcaccgtcaa
     1561 tgtgcggcca ccaaactccg tagagttcaa tgctgcaatt gcggcttgcg cttcctctcc
     1621 ggtagacatc tcaacaaatc cgaatccgcg cgattggccg gtgaacttgt cgctaatgac
     1681 gttagctgat tcgacggtgc cgtgtgccga aaacaggtcg gttaattctg cttgagtcgc
     1741 tgaatacggc aacccaccta catacaattt tgaacccatg gggttcctcc ttttaaaaag
     1801 atgatattgt ctgatgctcg agaagggtaa aacatttgga aggggtaggc cacggacgct
     1861 gtttccacgc cactcaggtc agacacttcc gagttattct ctcgggctaa acaacaccgg
     1921 ttacgggctt gagtgattga actctcaaag ctaagcccag ggcgatgagt taaaaaatcg
     1981 tagcacacaa tggaagtaaa agatagtagc gaaaaaatgg cactattacc tataaatgga
     2041 tagatccgat ggtgctatcc ggaatggacg gcctaaccgg agtgcttcac ggttgtacgg
     2101 ttccgccggt gacgcgttcc agttcggtca gggcaatcga gagatcgtat ttggcctgcg
     2161 catagtctat ctgtgtttgc cgcaagaccc gttgagcatc cagcacatcc aacagactgg
     2221 cctccccgta tttaaagctg acctgggcga tgcgtagcgc ctcttgtgcc tgcttgagca
     2281 gccctttttc gtatgtggca atttgtgcgg ccgcggcttg agaaagttga atctgctggg
     2341 cgatgccttt gagaagttcc tgcttggccc gaaggaggct cgcttcccgt tgccgcaagg
     2401 tgcctttggc ctcggcaatg tccccctggc gttgattcca caccggaaaa ggtatggaaa
     2461 ccccagcgat ataggcttcc cgccctgcgt ctcgttggta acctccgctc agcgtcacat
     2521 cgggcacgcg agcctgttgc tcctgacggt gggtctgctg cgcttgtacg atgagttttt
     2581 tccatttggt aatgatggga tgatttgcca gagtctgctc cgagagtgtc tcgaggtgta
     2641 aggctccagg ccagacggcg aaatcacctt gaatggagaa ttcaagtccc aatgccccgg
     2701 cagtgaggct attgagtgag gcttggatgg actggacgac acccctggtc cgggcgacct
     2761 ctttctgaac tttcatacct tcaaccttga ttttcacggc atcaaaaggg ggcgcttcac
     2821 cggcctcaac ccgacgaatc acggcctttc ggacttcttc catcgtctga acattttgga
     2881 tggccgtttc cgtttggcgt tcggcaaata agaggtcata gaaagcctgt ttggtggccg
     2941 cgattaaatt cagtttggct tcttcttttg aggccagggc gccgtccaat cctgcctggg
     3001 ctgcctgttg gcgtgccgcc cgtttccccg gccattcgat gggctggtag agtgtgacgt
     3061 accgttcggt gagagtgatg ccggtcggat cacgaacggt ccctcgtccg ctctgaaggg
     3121 caatggtggg attgggatag gcggaggcgg agattttgtt cccttccttt tgttcgatga
     3181 ccccctcgct ttcgttcatg aggggatttt tctctaaggc gatggccaga atatcttgca
     3241 ggctatagct tggggccgca gcctgcggtg tggggacagt cgctgccaac acggattgcc
     3301 agcagaaacc gtatagcatg atgaggcaaa tgatttgttt gaaccacatg acgtgttccc
     3361 ttcttgtgac aacaccatcc ttttcagatt gaggtggtgt tggtgtgaga ctgatggggt
     3421 atggctggtt gtttgttgga acggcagacg gtgccgtaca gcgctggaat aataaatagg
     3481 gtcaaggctg tggaggtgat caaaccgccg atcacgactg tggccaaagg ccgttggacc
     3541 tccgctccca tactggtggc gacggccatt ggtaaaaacc ccagactggc gacgagggcg
     3601 gtcatcaaaa cagggcgaag acgatccatg gctccctgtg ccacggcttc gcaggtcgga
     3661 agcccttcgg tttccagcct ctgaatacgg ctcaccaaaa cgacaccgtt taagacggcg
     3721 atcccgaaga gggccacaca gccgatgacc gccgagatac tcaacggcag gcctcgcatc
     3781 caaagggcaa aaataccgcc cgacagagcg aggggcacat tcagaaatat gagcaaggcc
     3841 ggacgaatcg ttccaaaaat caccgaaaga atggcgatga taagcagtaa ggtaatcggc
     3901 accactacag ccaaccgccg tgaggcctcc tggagatgtt taaattgtcc actccattca
     3961 aggtagtacc cgggtgggag ggagacgcgg tcgcggataa cctgttgggc ttcggtgaca
     4021 aatgagccga ggtcccgacc acggatattg gcttcgacga ccagacggcg ttgaatattt
     4081 tctcgactga cttgcgcagg gccttcatcg acttcaatgg tcgcgacgcg ggacaaggga
     4141 accagttcac catgcgcggt ggggatcagg atgttgccaa gagctatggg cccggccgat
     4201 gtctcatcag agaggcggac aattagatca aagcggcgtt gcccctgaaa gacggtcccg
     4261 acagtttggc cgacccgggc ggattcaatg atacctaaca cgtctctggc gctcagcccg
     4321 tatcgcccga tttggtcacg gtcgacgatg atccgaatcc tggggacacc ggtgacttgt
     4381 tccactttca catcggctgc gccgggcacc tgttcgagtg cacgtgccac cccatcaccc
     4441 tgatggcgta agacctcaag atcctctccg aaaattttga tagcgagatc ggatcgggtg
     4501 ccggcaatga gttcattgaa tcgcatctca atcggctgag tgaaactgag tcccactccg
     4561 ggaacggctg ccagaacggc ggttttaaaa ttttcgatca acgtctcttt tgaagtggtc
     4621 gtggtccatt cgttccgagg cttcaggatg acaaagacat cagacatatc aatgcccatc
     4681 acgtcggtgg ccacttccgg acttcccgtt ctggtcacca cctggacgac ctcgggaaat
     4741 tggcgtaacg cccgttccac gcccaaggcg gtttctacgg attcggtcag ggagacgctt
     4801 gggaggcgcc agacttgaat ggccatatcg ccttcctcta atcgaggaac aaactcgatc
     4861 cccaagcggg atccgatcac cagactgaga ataaacagga ggatgccagg aataaccacc
     4921 agcgccggcc ggttcaacga gaccgctaac catggtcgat agagccggcg caaacgccga
     4981 atgagccagg tttcttcatg gttcggccgg gcgcgggcaa accaatacga cagcacaggc
     5041 acgcctccta ccgcaaagag cagtgaggcg gccagggcaa aaatgacggt caaggccatc
     5101 ggccgaaaca ttttgccttc taacccgatc aacgacaaaa taggaagata caccacgatg
     5161 atgatgccga ctgcaaacac aataggccgc aacacttccc gtgcggcttc ctgaatcgtc
     5221 gccaacttct cttccttcga ttggcctgtg acgtttccca gccgtcgcag aatgttatcg
     5281 atcatgacga ccgatccatc cacgagcagt ccaaagtcga tggctcccag gctcatcagg
     5341 ttgccggaga tgccggcgtg atacatgccg gtgaaggcca tcagcatgga aagaggaatg
     5401 gccaacgcca caatacatcc ggcccgaatg tcgcctaaaa atgaaaagag aatcgcgatg
     5461 actagcagac ccccttcgag taaattattt ctgacggtgt tgatgacctt tgagacgaag
     5521 agggtccggt cataataggg ttcaatcacc actcccggtg gaagggtggc ttgaatttcc
     5581 tggatgcgtt ctttcacacg tttgaccact tgctgggcat tggcgcctgc gagcatttga
     5641 accatcccga tgacggtttc cccctgtccc atccgtgtgg ccgcgccaat ccgcaaggcg
     5701 gcgccttctt tgacttctcc aagatctcta atgaaaatgg gaacgccacc cggcccatga
     5761 ctcacgacaa tttgccctaa atcggtaatc gtcgaagcca tcgcttctcc cttaatgaga
     5821 tattgttccc gctcatgttc aatgtacccg cctccggcga gggcgttatt ctgcgccagt
     5881 gcatcaaaca cggtttttaa ggtgagatta aaggcgagga gcttgccggg gtcgaccacc
     5941 acttgaaatt gtttggtttc tcctccccaa atattcactt ccaccacacc gggaacggcc
     6001 cgaagccgcc tgccaatatc ccattccaga agagttctca agaacatggg actatgcccc
     6061 tggcctgtga gcgtgaattg gtagacttcg ccgaggccgg ttgataaggg gcccatcatc
     6121 ggtcgtccat aggcggcggg aatgcgttcg gtggctaacg tcagtcgctc gttcacgagt
     6181 tgccggacaa aatagagatt caggtgttcc tcaaagattg ccgtcacggc cgataagcca
     6241 taacgtgaga ccgagcgcac tttctgtaat cccggtatgc cactgagtgc ggtttcaata
     6301 ggaaatgtga tgaattgttc cacctcaatc ggaccgagcg cgggggaacg ggtgaggatc
     6361 tgcacctgga caggggtgag atcggggacg gcatcgatcg tgagattggt cagcgaccag
     6421 actccggagg cgatcaccag aagcagggca acgacggtaa aaaaccgata ccgaagcgag
     6481 agggtaagaa gacgctccat taacagggct caatagtgtg aaaagggcaa agtgaaatgt
     6541 tcagggggat gctccttacc attaactttt ttcttttgac ggctcattct ccctggatgg
     6601 aggttttcat aatggcgttt ttcaggtcga agacaccttc ggtgaccacg tgttctccta
     6661 acttcaaacc ccgtttgatt tctacaagtc cggcgccttc ccggccggtc tcgacaaaca
     6721 cgaaatcgta tccgttgtcc cgttggacga acacgccgcg cacgccctcc aacatcgtta
     6781 aggccgataa cggtatggat aacgcgggat gttgttgatc gatcaagcgc acttccacat
     6841 attcattggg tttgagtcgg cccttggggt tttcgacgtc gaatcgcacg cggatcgtcc
     6901 gggtggtggc atccgcgacc ggggagatat aggtgagtcg ggcggtgatg ggctctccgc
     6961 cacggggaag gacctggcct tcatcgcctt cccggaaccg acgggcttgt tcgatgggca
     7021 gattggcgaa cacccacacg cgactggtat ccacaatttc aaagaactcg tctcctgccg
     7081 tcacgccctg gcccagcacg atctgttggc tcaccacggt tccgctgagt ggggcccgta
     7141 gtaaatattt gtgcccttcc agatgactgc catgctccag ttgctggagt tcttgttggc
     7201 tgagtcccat gtttaggagc ttttcccgca cgtgctgata gacggcctgg gcttcaatct
     7261 gctgtccgcg gtcttccaga aaccgacgtt gggaaataat gttttcagcc aggagttgtt
     7321 tggttcgctt aaagttgctg gaagccacat ccaatttgga tttggccacc agatattcct
     7381 ggactaattc atccaagcgc atgctttcca cggccgcgag cggctgatcc tttgtaaccc
     7441 gatctcctaa ttgcaccata accctatcca cttgtccggg gatgcgagag gaaacttttg
     7501 ccacgtgcgc gagatcgaga gcaacatttc ccggagccga aactgaatgc ggggtcaggc
     7561 gttctttgac ttcttcaatt cggattcgtt gacgcgcttg ttcagggaca tagtccacgt
     7621 gaaagggttg tcccggagag gaagagggat cgggggagga agccgtcacg cttgtgtccg
     7681 gtaggtcgcc ggttggtggc tgggtgtcgg agcatcccat cggaaacagc ccgataagac
     7741 agagaacagg gagccatctc cagtgtctgt gattcagatt cttcatgaat ggcccaattg
     7801 gacaaaaaat ctcctacagg aaaatactat catggtgctc ctcttagcgg tggttcatca
     7861 ccggttggga gtgtgatcgt gaatgtggtg ccctttccta tttgactctg cgcatgaata
     7921 gttcctccgt gggcttcgat aatctccttg acgatcgcca atcccagacc tgttcctcct
     7981 gattggcgat cccgtgcctg gtcgattcga tagaatcgcc caaaaaggtg agggagatgt
     8041 tgggaatcaa ttcccggtcc ggtgtcctcc acggataacc ggatggatct atcctgagcc
     8101 ccgacccgaa cggtgatggt tccatctgaa ggcgtatgcc ggagcgcatt atccaggaga
     8161 ttgatcaggg cttgtttgag ctgtgaggca tctcccataa gaggaggaac agaatccaac
     8221 tccgtctgca gatggatacc gccttcatga gctaacacgg acaattcgca tacgatttct
     8281 ttgacaagag gcgggaggtc aagggattgc aagacgagtg ggggacggtc gccggcgaat
     8341 tgtgccaggg tgaggaggga tttggtcatg gcggtgagtc ggtcgacttc agccaggttg
     8401 gagagaatgg tctcccggta ttcctctgcc gaccgtgccc gttgcaagct gacctcaaaa
     8461 ttgcctttca tcgccgttaa cggggttttc agttcatggg ctgcatcggc cacgaagcga
     8521 cgttgtcctt caaagacctg ctgcaagcgg tccaacatgc cgttaaaggt gtgggcgagg
     8581 cgttgaaatt cggcgtaggg cgagctaagt gtgagacgag tggaaatcgt ctgcccggac
     8641 acggtttccg ccgtctgact caatgccctg acgggagaca aggcttcatc ggccaatcgt
     8701 ccgcttcccc ataaggcaaa caggagggaa atcgcggagg tgccacagag tagccacgcc
     8761 aaccactgaa gggtttcctg aacaaattgc aaggattttt ccgtttgtaa aatgaatcgc
     8821 ccgtggtctt cgatgacgag aggaattgag atacggcgaa tgggcggatg atccggcttg
     8881 tgaaaggttt caaaaacggt ttctccattg aggacctgca tgataagttg ggacgtcaca
     8941 ggaaggcggt tctctgtgct ctgcccactc caaagaatac ggccggcgga atccagaata
     9001 gcgctgtagc ggatagcctg ctgtaactca tcttcgtcat caaattcttc taattcgtct
     9061 tcttcatgtt tccggtgagg agacacacga acatggtccg ttgccaggtc cacgcggagg
     9121 ctttcctcct gtgcgagtgc catcagttcg ccatcgatat ggcggtgcaa aattgtagat
     9181 agtcccaggt acaggagcag gctaaagagg cctaggagga tgacgatgaa gcctagggcg
     9241 acacgacgta tgcgagtgcg gaaggcctgc atggtttagg cctccgggat ttttaaaaca
     9301 tagccgatcc cccgcacagt atggatcagc tgtggagaaa agtcccgatc cattttgctg
     9361 cgcaaggttt taatgtgcac atccacaata ttggtaacgg actcatagtg aatgtcccac
     9421 acgtgttccg tgatggctgt tcgggtcaat actcgtccgg tatttctcat gagatattcc
     9481 aaaagtgaat attctttgtt ggtcagaatg atggtttggc ctgcccgtga aacacggtgg
     9541 gttacaggat ccagctccag gtccgcgatc gtgaggcgag ggctgggctg ggcactgcca
     9601 cgacggagaa gcgctctgag gcgtgccaat aattcggcaa aggcaaacgg tttcgttaaa
     9661 taatcatcgg cgccggtatc cagaccggtc actcgatctt caatggcctc ccgtgcggtc
     9721 aagagaagaa tcggggtcgt gtggcgtttc gctcgaagcc ggcggcagac gtcaatgccg
     9781 ttcattttgg ggagcataat atccagaatg atcacgtcat agcgggtggt ttcggccatg
     9841 gccaatccca tctcaccgtc tgtggcgaga tccacagcat attgctcttc ccgcaagcct
     9901 ttcacgataa acccggacac acttgaatca tcttccacta agagaatacg cattggtttc
     9961 ccgcagaaaa tttcaatcgg taaggttggg ttttctccgg cacattgagt cctgctttgt
    10021 gcgcaagggc aacccctgct ccctgcccgt agaccaaatc tccatcatag tgacccgtgg
    10081 cgcctagtgt gcctaatccg atcgccacaa tcaaaaagta cgggactatg gtctttcgcg
    10141 taaattggtt tcacaacacc aggcgaccca ataggaggac tccaaaaata cccaaggtca
    10201 cgaaggcaag cgtctcgtaa gtctcaatca tcgattcggc aattccggcc ttttcagcag
    10261 cttcctctgc ccaatcgccg gccatggcgg cagccacccc tccaagaaga ccgagactaa
    10321 gaagccataa ggcaccgtca cgaaagttct cacgtttgaa ccaggctcct gcggcatcaa
    10381 atagcacact ggtgaagagt aaggcgatgg ggaaatgaac taacatggga tgtcatcgca
    10441 actcaaacat aatttctcct gtgtgtcgtc aaagtggtga agcgagagaa ggccccatac
    10501 cctcttgcgt atggggcctt ccggttctct taggattttt cggtatcctt gtgagaagcg
    10561 cccttctcgt gcccttcctc gttctcgagt tcagagctca gaatttttcc cgattgggcg
    10621 tcaatctcaa attccctgat ctctccggcc gcgttgacga tctcgacctc atacctcacc
    10681 ttcccgtctt cactttccag ctcggcttcc agcaccttcc ctggaaattg agtcgtcgcc
    10741 gttttgatgg cttcttccat ggtgaccgac gtggccaggt tggccacggc tgctttgtct
    10801 ttgccatcat gactcatggc caatccaaca gaagtcaata acaacgctga ggtgcccaat
    10861 agactgattg tgtgtaaccg cttcatgata catcctcctt agtgaaaaat taatgaaagc
    10921 aaccggctgt tcctgaatac agcatagact gtctacatga agacgatatg aagcggacat
    10981 gaaagtttct tcatcatcat attgaggatg acgtccgtca cggtaggccc ttgttgcggc
    11041 catgtcggac aggccgaaaa atagcggttg ggaaggatga atgcgttgtt tgttgaatcc
    11101 gaatgtgcgc gccaacatgg cggcaaggaa aagccggtct agacgttgaa tttgaagtgg
    11161 cagacgtcgc cgtctttgac gacgtattct ttgccttccg accggatcag gcccttctcg
    11221 cgcagggcgg cttccgactt gtatttgtcg atgtcctcga aggcgtaaat gtcggccttg
    11281 ataaagccct tctcgaagtc ggtgtggatg acgccggcgg cctgtggcgc cttggctccg
    11341 atgggaatgg tccaggctcg gacctcctgc actccggagg tgaggaacga gcagagcccg
    11401 agtgttttat aagcggcaac gacgaccttc tccaagccgc tctgctccat gcccaaatct
    11461 ttcatgaaaa tttcgcggtc ttcccctgac agctgggcga tttcgctttc gagtttgcca
    11521 cagatcacga ccgattccga accgcgttcc ttcgcgaatg catgaaaatc ggcaaggatg
    11581 gctggatccg gattctcgtc ggtatttccg acatagagca cgggcttcgt cgtgagcagg
    11641 aaaaagttct tgaggacttc cggctcgagg cccagggctc gcgcaggttt acccgcttcg
    11701 aggccgctct tcagaatatc gagcaccaca agagcctctt tcgaagcctt gtctccggac
    11761 ttcgccttgg ccgtgacctt gtcgtactgt ttggtgagac tgtccaggtc cgcgagcatt
    11821 aattcagttt caatcacctc gatatcacga cgatggtcca cgccgcccat ggtgtgcacc
    11881 acatcgggat cctgaaagag tcgtaccatg tgaaggatgg cgtcaacttc ccgaatattc
    11941 gcaaggaatt tattccccag cccttcgccc tgagaggcgc ctttaaccag accggcgata
    12001 tcgacgaaac ggcaggaagt cggcacggtt ttcttgggtt tgaccaactc ggtcaggcgc
    12061 ccaagacgcg cgtcaggaac ggccacgaca ccgacgttgg gctcgatggt cgtaaacgga
    12121 tagttcgatg cggccgcgtt tccagaggtc aacgcgttaa aaatcgtaga tttgccgacg
    12181 ttgggtagac cgacgatgcc aatttccata ggaggtgcaa ggtatcaaat tagaagcctg
    12241 caggtccttg agccaaaagg cctataggtg cccgctcatg ctctcgtcgt aaaagtcatc
    12301 aaaaggtgaa caatctttct gccgtttccc cgtactctct ccttacgttg aaacagaaaa
    12361 ctttactatc gatcaattca ggcatggaga aagcgggatg ttccggcgat ttcggaatag
    12421 caaattttga atgtggtcac tccgctgtag ttgaaaggat aatggtgtgt aaaggttttt
    12481 gaaaatccac ttcaggtcgt ttgtcctggg atccagtgga cggcgtggtc ttaggaagac
    12541 cgtagggcca aggcaacttg caaccagaac acgcatggta aggggtctgt gaagagaggg
    12601 gatgacagtc ctttatgaat ggtaagccaa ccaacggctg ccgtgcaggt ttgggaatga
    12661 agtatccaga caaatccgga ttccgacggg gtatcctaca aaaccggcaa gtctggatag
    12721 tgtgtgggca attgagggcc ggaatctttg ttcgttcctt tttccaaaac gagtcgcaac
    12781 cggcttgttt tttcttgact taattcgttg gttaaagaaa aattgatcaa ggtggggagc
    12841 agttgtgtag tgggatttgg gcaaaagaag aaggcgccga atttacctta aggccaaggc
    12901 tcccagagtg tggtggtgag aatgtggaaa ggcattgcgg caaaaattaa ggcactatgg
    12961 tcatgaaatg aaagttcaac tgtattcatc atgaaatctc aatgtcctta ataatattga
    13021 ggaaaacagc gcttcgcagt tgaggaggat cagtctcaag attgtctggg attcactaat
    13081 gcccgagaaa tcgcgacgtc catcgcaggg aggagcggtc atgcaggagg acaagaatca
    13141 gattgtgtcg ggtatactca gagcccgggc ggagttggag caggtattgt atgaaatgga
    13201 gaaactgccg gccatcagtg agagtgccgt ccattttgcg gcacatgcac tgaataacta
    13261 tctcaccgtg accggtggga ctgttgaatt actcctcttg atgctggcga atcatccgga
    13321 caagcaggtc cgcaccgggc ttgaggcgtt gcaacaggcc acgaatttga tggcgcacac
    13381 ggtcagtcga ctggtaaatg ccgagtccgg tctggatgcc gaaatgcgct tcgaaaaggt
    13441 ggagttgccg attatggcgc agcggttccg ccttttttac caacgcattg cggatcagaa
    13501 gcagattcag tgtcttgtag ggtccgcggt cgatgttcct cctgtgtgga ctgatcgtgt
    13561 cgccaccgcc gctgcgctcg ataacctgtt ctcgaacgcc gtgaagtatt caccgccggg
    13621 aaagcgcatc tgggtcgaag tgttgcccca gcaggattgg gttgtctgtc gcgtccgcga
    13681 cgagggtcca ggcctgagtc cggaagacca ggccaaactc ttccagcgcg gaatactact
    13741 cacccctcga cctacgggtg gagaaccttc aacagggtat ggcttggcag tcgccaagga
    13801 actgatcgag aaggtagggg gagaaatctg gtgtgagagt gtgttgggac agggggcctg
    13861 tttctctttt cgattgcctc aataccagga actggttcat ggatccgatg gaatggtgcc
    13921 aggttcccat gagggcagga aacatgaaag ccgaactgta cgccacacct gatccgccag
    13981 gtgagctatt tgtgttagtc acatcacgat cgtgcacatt catttctgta aatcccgtaa
    14041 aggggaaaca ccaatgagat cgacacgacc catccg
//
LOCUS       DATIAL010000511         4352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1489661, whole genome shotgun sequence.
ACCESSION   DATIAL010000511 DATIAL010000000
VERSION     DATIAL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489661"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VLA60_15665"
     CDS             complement(<1..461)
                     /locus_tag="VLA60_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10849.1"
                     /translation="MPATQLQFYQVGSYPYPYATTGTGSALTVCRRYNDPYLAFLTQT
                     SYEDEIVFLYLHSSVSQESGRLQTPPGHDVISGMDYAIFRNIIWAVQSTQNQDTIIGI
                     DPETGNVTDTILVLPRSGAALAYNGLYFVRSDGNVLEAISNSGVVIGTVNVP"
     gene            complement(936..2504)
                     /locus_tag="VLA60_15670"
     CDS             complement(936..2504)
                     /locus_tag="VLA60_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HSF10850.1"
                     /translation="MDDPIRTEMMKHRLVSVAEEMGARLQRAAFSPNIKERCDFSCAV
                     FDASGDLVAQAAHIPVHLGAMPLSVQACLESLTLAPGDVAMVNDPYRGGTHLPDLTVV
                     SPVFVAGTTPPTLLGLVANRAHHADIGGMSAGSMPLSQEIFQEGLIIPPVKLMVGDKK
                     NDDIWRMLLANVRTPEERMGDLQAQLAANQIGGERMRVMAERFGVQPLLAEMDALLRY
                     SERMTRQLISTLPNGCYRFEDALDNDGFTEKPADIRVAIMIEDEQVTVDFSGTDAQRP
                     GSINAVYPVTLSAVAYVFRCVLGLDSPANSGCLRPVHIIAPEGTLVNARPPAAVAAGN
                     VETSQRIVDVLLGALAQACPDRIPAASQGTMNNLTIGGWDQRLKRPFAYYETIGGGMG
                     AGPQFDGASGRHSHMTNTLNTPVEAVEYAYPFRIVRYALREKSGGSGKHQGGEGIVRT
                     YEFLQPAEVTLLSDRRITNPYGLKGGAPGQPGRNQLKQNGIEKDVSGKCSFPVEAGDQ
                     LTIETPGGGGYGIN"
     gene            complement(2497..>4352)
                     /locus_tag="VLA60_15675"
     CDS             complement(2497..>4352)
                     /locus_tag="VLA60_15675"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HSF10851.1"
                     /translation="PGRHIVHGSTVATNAILERKGARTALITTKGFRDVLLIGRQNRP
                     ELYDLFPVISQPLVPREWSFEISERVDCEGNILTPLQEQGLVSILEMLRHEAIQSVAV
                     SFLFSFVNPAHEQWVTRRLRDEGFFVTGSSEILPEFREYERTSTTVVNAYVSPVLDTY
                     LGRLEREIKPTEFHIVQSNGGRISVAQARGHGVRSILSGPAGGVVGARYLAGLAGFTR
                     ILTYDMGGTSTDVALVHETIHVTTEAKVGGNPIRVPVIDIHTVGAGGGSLATVDAGGN
                     LRVGPQSAGAQPGPVCYGRGGTIPTVTDAHVLLGRLPTDGFLGGRMRLNVESAQQAFD
                     ELSRQMVLAPRSGLDIRQTLALGMIQIANVHMERALRVISVERGEDPRETVLVAFGGA
                     GGLHACDLARALGIPHVLVSPMAATLSALGMLAAEVQLDYVQTVMLRGDTPIEILEQC
                     TQPLVDQGQKDLHREGVDPDQWILSRTVDVRYVGQSFDLAVPLTSMFREEFHRIHHTR
                     YGYSQTETPIEIVNVRVRAVGHVAPPTIPARPLGSADPTAAQWEDRPVVLPQGMQPVP
                     HYWGARLRPGHEIHGPAILVLDDTTIYLGPTDHAEIDTYLNILIKVGAPDG"
BASE COUNT         1045 a         1254 c         1090 g          963 t
ORIGIN      
        1 gggacattca cggttcctat gacaactcca gaattgctaa tcgcctcgag cacgttgcca
       61 tcggaacgca caaaatacaa accgttatac gccagagccg ccccgcttct cggtagaaca
      121 agaatcgtat ccgtcacgtt cccggtctct ggatcgatgc caatgatcgt gtcctgattc
      181 tgtgtcgatt gcacagccca aataatattt cgaaaaatgg catagtccat cccactaatg
      241 acatcatgac cgggaggtgt ctgaaggcga ccggattcct gcgacacact cgagtgcaaa
      301 tacagaaaca caatttcatc ctcataggag gtctgagtga gaaacgccaa atagggatcg
      361 ttgtatcgcc ggcagacggt taacgcactg ccggttccgg ttgtcgcata aggatagggg
      421 tatgagccca cttgatagaa ttgaagctgt gtggcaggca tggtcttctc cttttttggc
      481 tcgttgtcaa aacctgtacc tgcacctgaa gttgatacaa agtcctttca acagatttcc
      541 catttgtcat accgatcaag tgctacgccc caatgaacca cgatattcat tctctctcgc
      601 tgttatattc acttttttct gtcccattgt agagtaacag caaaacccat tcctcaggtt
      661 gacgactaat gggcaaagac cctaactact taaaactatt gcaccgttaa gacaaaggaa
      721 cggtgatgac aaaattaatg aactgaatgg ggtggattcg gttaggccct gagatacgat
      781 ttgtatctat tcagatacga ggttgttgat gagttccatg agggatttgt ctaggactag
      841 gagtatcaga cgggaagacc caggctcatg aatgaggctt ttaatttaat gatttccata
      901 attttggagt ccctttttcc caacttacca ggattctagt tgattccgta tccgccgccg
      961 ccgggagttt cgatggtgag ttggtcgccg gcttcaacgg ggaaggagca ttttccactg
     1021 acatcttttt cgattccgtt ttgcttcaat tgatttcgac ccggttgtcc cggtgcgccc
     1081 cccttcaatc catagggatt ggtaatccgt cggtcggaca acagcgtcac ctcggcaggc
     1141 tgaagaaatt cgtatgtccg cacgatcccc tcgccgcctt ggtgcttacc gcttcctccg
     1201 gacttttccc gcaaggcata acgcacgatt cgaaacggat aggcgtactc aaccgcttcc
     1261 accggcgtgt tcagggtatt ggtcatgtgt gagtggcgtc cactcgcccc atcgaattga
     1321 ggacccgcgc ccatcccccc gccgatggtt tcgtaatagg caaagggtcg tttgagtctt
     1381 tggtcccatc caccgatggt gagattattc atcgtgccct ggctggccgc cggaatgcga
     1441 tcaggacagg cttgcgccag ggctccaagt aaaacatcca cgattcgctg ggaggtttcg
     1501 acatttccag ccgccaccgc ggcaggaggt ctggcgttta ccaatgtgcc ttccggcgcg
     1561 atgatgtgaa ccggccgcaa acatccgcta ttggcagggc tatccaaccc caatacacat
     1621 cggaacacat aggccactgc ggacaacgtg accggataga ctgcattgat gctaccaggc
     1681 cgttgggcat cggttcccga aaaatcgact gtgacctgtt catcttcaat catgatggcc
     1741 acacgaatgt cggcaggttt ctccgtgaat ccatcgttat ccagggcatc ttcaaaacgg
     1801 tagcagccat tgggtaacgt actgattaac tggcgggtca tgcgttcgct gtaacgaagc
     1861 agggcatcca tttccgcgag cagcggttgc acgccaaacc gttcagccat cactcgcatc
     1921 cgttcacctc caatctgatt cgcggccaat tgagcctgga ggtcacccat gcgttcctct
     1981 ggagtacgga cattcgccaa taacatccgc cagatgtcat catttttttt atcgcccacc
     2041 atgagtttca ccgggggaat aatgagtcct tcttgaaaaa tttcctgtga caacggcatc
     2101 gaccccgccg acattccccc aatgtcggca tggtgcgcac gattggcgac caaccccaag
     2161 agagtgggag gagtggtgcc tgccacaaaa acgggcgaca ccacggtgag atcaggaaga
     2221 tgtgtgcccc cgcgataggg atcattgacc atggccacat cgccaggcgc caacgtcaag
     2281 gactcaagac acgcttgcac agacaagggc atggctccaa gatgaacagg gatatgcgcc
     2341 gcttgcgcga ccagatctcc ggatgcatca aacaccgcac aggaaaaatc acagcgttct
     2401 ttaatgttgg gcgagaaggc ggccctctgc aggcgagcgc ccatttcctc ggccacggac
     2461 accaaccggt gcttcatcat ttcagtgcga atgggatcat ccatcggggg ctccaacctt
     2521 tatgagaata ttcaaatacg tatcaatctc ggcgtgatcc gtgggtccaa gatagatggt
     2581 ggtatcatcc agaaccagaa tggccggtcc atggatttca tgtcccggac gcaagcgggc
     2641 tccccaataa tgcgggaccg gttgcatccc ttgaggcagg accaccggac gatcttccca
     2701 ttgagctgca gtcggatcgg ctgaccctag cggcctggcg ggaatggtgg gcggcgccac
     2761 atggccgact gcccggacgc gtacattcac gatttcgata ggcgtctctg tttgactata
     2821 gccatagcgc gtgtgatgaa ttcgatgaaa ttcctcgcgg aacattgaag tcagcggcac
     2881 cgctaagtcg aaggattgac cgacataacg cacatcgacc gttcgagaga gaatccattg
     2941 gtcaggatcc accccttccc gatgtaagtc cttttgcccc tgatcaacca acggttgcgt
     3001 gcactgctca agaatctcaa tcggcgtgtc cccccgcagc atcaccgtct ggacataatc
     3061 caattgcacc tccgcagcta acatgcccag agcagagagc gtggcggcca tgggcgacac
     3121 caacacgtgt ggaataccca gtgctctggc cagatcgcag gcatgtaatc cgccggcccc
     3181 accgaatgcc accaggacag tttccctggg atcttctccc cgctccaccg aaatgacacg
     3241 cagtgcgcgc tccatatgga cattggcaat ttgaatcata cccaaggcca aggtctgccg
     3301 gatatccaag ccggaacgag gggccaaaac catctgccgg ctcagttcat caaacgcctg
     3361 ttgcgccgat tcgacattca atctcatccg gccacctaaa aacccgtcgg tcggcaatcg
     3421 ccctaaaagt acatgagcat ccgtgaccgt cggtatggtc ccgccccgcc cataacacac
     3481 cggacccggt tgcgctccgg cactttgagg tcccacacga agattgccgc ccgcatcgac
     3541 ggtggccaga gagccacctc cggcaccgac cgtatgaata tcgatgacag gcacacgaat
     3601 gggatttcct ccgactttgg cttcggtcgt gacatgaatc gtctcatgaa ccaacgccac
     3661 gtctgtggac gtcccgccca tgtcatacgt cagaatccgg gtgaagcccg ccaacccagc
     3721 caggtatcgc gcgcccacca cacctccggc cggaccagac agaatggaac ggacgccatg
     3781 ccctcgcgcc tgggcgaccg aaatacgccc cccgttcgat tggacaatat gaaattcggt
     3841 cggttttatc tcccgttcta accggcccaa ataggtatca agaaccggag acacataggc
     3901 gttcaccacc gtcgtactgg ttcgttcata ttcccgaaat tccggcagga tttcggaaga
     3961 acccgtcaca aagaatcctt cgtcacgcaa tcgccttgtt acccattgct catgcgcagg
     4021 attcacaaag gaaaacagaa acgatacggc caccgactga atcgcctcat gccgtaacat
     4081 ttcaagaatt gagacgaggc cttgttcctg aagaggcgtc aggatgttcc cttcacaatc
     4141 cacccgctcc gagatttcaa atgaccattc acgcgggact aagggctgag aaatgacagg
     4201 gaacaggtcg tacaactcgg gacgattttg ccgtccgatc agcagcacat cgcgaaagcc
     4261 ttttgtcgta atgagggccg tccgcgctcc tttgcgctcc agaatggcat tggtggcgac
     4321 ggtggaacca tgcacaatat gacgaccagg at
//
LOCUS       DATIAL010000512        11140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_735851, whole genome shotgun sequence.
ACCESSION   DATIAL010000512 DATIAL010000000
VERSION     DATIAL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11140
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735851"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VLA60_15680"
     CDS             <1..291
                     /locus_tag="VLA60_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10852.1"
                     /translation="RQAAYGFQSDLGRWKREPWGAGEVLVKSIVNDYVSVLITNRRAL
                     GFSALTGGFFPQDLPSGNVIDQTEANANIVIIHLSGLMLVFRSGLAIWTVLP"
     gene            complement(292..519)
                     /locus_tag="VLA60_15685"
     CDS             complement(292..519)
                     /locus_tag="VLA60_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10853.1"
                     /translation="MDTPHLPQPAPMPIFILFDTQTGYLYGHLFATGPEDPFLQEVID
                     WWSHYCSNSPIGVDYPGVTKHQELASSPASN"
     gene            812..2272
                     /locus_tag="VLA60_15690"
     CDS             812..2272
                     /locus_tag="VLA60_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF10854.1"
                     /translation="MDAFDSAGSAITKQVARILLVEDEVDVRESIKLQLEDEGHQVVD
                     TESGGDALILAESSQFDIVLTDVMIPDINGIELVQRITQLPNHPVMIVMTGYGSVEMA
                     VKAIKAGAFDFLSKPFSMDVLSATLASALRVKCLQDENVELKKTVKGQFSLGKLISSS
                     QVMQEVFQLIDCVADTDSTVLILGESGTGKELIAQTIHCHSTRRQGSLIPVNCGAIPE
                     ALLESELFGHEKGAFTGAVASRVGRFELATGGTIFLDEIGDLSPPLQVKLLRVLQERT
                     FERVGGTRTYKSDARVIAATNQDLETLVAAKQFREDLFYRLNVVPVLVPPLRHRVEDI
                     PLLLQHFITMFNDRRKAELTGVADDAMSLLCEYPWPGNVRELGNIVERIAILKRRGLI
                     VPEDLPEKIRRLPSSHLPTMPAAIPVAGMDLSRTVEEFENRLILEALERTNWVKSKAA
                     RLLQINRTTLIEKLKKKSLAEVVAQRATPSEDLADV"
     gene            2510..4588
                     /locus_tag="VLA60_15695"
     CDS             2510..4588
                     /locus_tag="VLA60_15695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10855.1"
                     /translation="MRKFTWIALLCVFWPSAVLGGPCSDFSWYVSRDMVMPEILRQGQ
                     DALRAGQLLEARDMFATYLKEQEHGQFAEGTRWVLASLPDPLDEPGKEKFQRIERLQA
                     MKMSDPESMYVPWAVCAMGNVYWEAGWFSEASGIFEDFLRSYPDHPLAGGVMVEAGLG
                     YLSNKQYLEAALILRRVVEEPKWSGHRLKAALGLADATAMAKAWKQAYYWYRVVEAEK
                     PELIRRSGNSAYHYGLTELAVGNPAMAISRFLLTVNLHPDEEPAGMALSQISEKLRKD
                     GHEYLGLYFADQARQRFPDQEPGRRGQAALTRWVVAFVTQEHAKEDWVKVYHRFDDLE
                     IYLSLSWDYVLETAGMLSHALERDVADESLLWMGQAYQALGEYADAVQTYTRLIVVTS
                     SDERRQTGQDRLARLLQHQMRSFYDSKAWVPLLKFHDDYQEAFQLVPLERERLFMVAQ
                     AYQQVKLPAEAWHWYGQLLKEYPDSPLREDIRLQQVVVAQEQENTSWVREAGASYLRE
                     FPNGQGRGQVETILALEALTDKRYAEAIHGFSAALQHVDGEVEQRYVLRNRARAYRAL
                     GQMESVVQDLRQVVAIQPTQVSDVLRLGDALFDHGDYVEAQHLYDQALAYDAPPALHT
                     WAKYRLAASLEYMGQSDEAAALLAEIRQLQTRSPELEHTIRSAATAVLDEMSSKETPL
                     TRIPDVPIQS"
     gene            4569..5732
                     /locus_tag="VLA60_15700"
     CDS             4569..5732
                     /locus_tag="VLA60_15700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10856.1"
                     /translation="MSQFNHRPMIALSSERNKELNWPTLDATTEPTISSETLSLALEA
                     KYAETQRLHHHLHRLVKSLPLPALLYNRKGRIVTANHEAHVLMGGIDWKKVNWRVQDL
                     WTSLGWPSVPFSDWKWKSGRVSCWDCPLGGMDQPSSLTVRYLKYEGDAQSGRTEQIER
                     LATIGEQVGRLAHDIRNPLASIEWFATLLGRDSPSGKGRRELADQLLHAIRSLEGLVS
                     NLLTSTTPLKGSRQCVNLLELLDDVELLATFPLRIKRLTIHRQRETSLLEILGHEQLL
                     KQALLNLLLNAIQASPPDGHIEIQCRRISRPAPGGAASAGVNGVAVNIRDEGCGMSEE
                     ELTRVFQPFYSKRQGGTGLGLPIVKDIMQVHQGMIEMESQTGKGTTVKLFFPQ"
     gene            5744..7132
                     /locus_tag="VLA60_15705"
     CDS             5744..7132
                     /locus_tag="VLA60_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF10857.1"
                     /translation="MIDTEGGVGRGILVVEDDEQLQSALTYALSQHGYAVTVARDGYE
                     GLERMEREAPWLVLTDLNLPGKGGMDFLREVRSQGYHMPVVVMTAYGGVDAAVEALKY
                     GATDFLQKPFPLDRLEKLIDQLKDAQPVSGLEVLREQERCRREEPREGFLTRDPKVLN
                     TISMLEMVAASPATILIQGESGTGKEVLARHVHRHSPRASQPFVAINCAALPEGLLES
                     ELFGYEKGAFTGALARRCGKFELAHQGTLLLDEIGEMSLGLQAKLLRVLQEREVDRLG
                     SRQPVQVDVRVIATTNRNLAQEVQAGRFREDVYYRLSVMPFTIPPLRDRPEDVQLLAE
                     YFANRSLRRNRRTPCEISEEAMSYLKRRPWRGNVRELENVIERGVLLAGNGPLLIEHF
                     QFEEPVLAANHASAPSGTIWEMERELILRTLERHDGNRTHAARTLGISIRTLRNKLRE
                     YRQLNGGLSLGI"
     gene            7447..7863
                     /gene="flgB"
                     /locus_tag="VLA60_15710"
     CDS             7447..7863
                     /gene="flgB"
                     /locus_tag="VLA60_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HSF10858.1"
                     /translation="MMISPWDEGTRLFERSLDVRSGIQETIASNMANEETPGYKSRIL
                     PFQETFNAVLRGDGPLESVVTNPKHLRTASEDSRIFQHTIKQDAGAGLDENTVNLEKE
                     MTLMAENTLMYMAVSQFLKGRFDGWNAAIDGGSGGR"
     gene            7992..8435
                     /gene="flgC"
                     /locus_tag="VLA60_15715"
     CDS             7992..8435
                     /gene="flgC"
                     /locus_tag="VLA60_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HSF10859.1"
                     /translation="MNIDRVFSVVGSALDAQRQRLNIIASNLANAESTRTPEGGPYIR
                     RDVVFQASPAGPQFSNVFANAFGDQSGPSGVRVTDVIQDERPLRTVYDPSHPDADEQG
                     YVHLPNVNPIEEMVNLMSATRAYEANLAVMETGKTMTLRALEMSR"
     gene            8470..8772
                     /gene="fliE"
                     /locus_tag="VLA60_15720"
     CDS             8470..8772
                     /gene="fliE"
                     /locus_tag="VLA60_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HSF10860.1"
                     /translation="MDYLPIKSIEGVSEPFGVTPLKGPAGQDQGFGDLLKKAVESVNA
                     MQHEAGRLEDAVARGENVNIHQAVIAGEKAGLSFKLLMQVRNKVIDAYQEVMRMQV"
     gene            8924..10516
                     /gene="fliF"
                     /locus_tag="VLA60_15725"
     CDS             8924..10516
                     /gene="fliF"
                     /locus_tag="VLA60_15725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HSF10861.1"
                     /translation="MDSLLSNFQALTLTQRIGVVVVLALALATIPMLMMVGRAPELVV
                     LFSQLNPDDTRAIIQELGKQGAVYEVGEGGNTIKVPAERVHELRLQLASLGLPESAGV
                     GFEIFDRTGLGVTPFTQQMNYRRALQGELARTIGQLSQVERVRVHLVMPEKRLFATEQ
                     KPAQAAVVLTLKRGAPLGGTQVQGIVHLVASSVEGLEPGQVTVVDNHGQVLSQNSADS
                     DAQLTASQIETQRRVERDLEQRVQTMLDQVLGRDKSVIRVTAPLEFRQVEITEESFDP
                     NSQVVRSENRSQEKVLESGSTHGGPGVPGVRSNVPSELKAGNGTEQKEAKRKNETLNY
                     EVNRKVSKIIEPTGAIKRLSVAVLVDGTYEAAQDAEGQGTGSTSEKKYVPRPEQEIQN
                     LVQIVKKAVGFSEERGDQIEVINVPFESPAILEGEESLTAGVQSFLATWGGFLKPVLF
                     FFLGVMVLWFVVRPMALNLTKPAAAAVVLPQKGLPATVTEYEAEISETPQEQAIKLAA
                     DNPASAAQVIRTWIKEEQGEKV"
     gene            10520..>11140
                     /locus_tag="VLA60_15730"
     CDS             10520..>11140
                     /locus_tag="VLA60_15730"
                     /inference="COORDINATES: protein motif:HMM:NF026192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10862.1"
                     /translation="MNSQLSGYEKAAILLLAIGESAAVEVLKVLDQKDIRQIGAYLGA
                     LVNVGQDEHRLVLKEFRELAGTSGLSVEGKTYLSKILNAALGKDKARRILSSLNTSEN
                     AGFETLKSLDAASIANLLAIEHPQTGALILANLESDHAAQILSLLPANKRDAIVYRLA
                     TTEEVSPNMLDELNAVLQEELRLGSSKNAVAVGGTGLVAEILNSVKR"
BASE COUNT         2680 a         2554 c         3319 g         2587 t
ORIGIN      
        1 cgacaggcgg cttacggttt tcaaagtgat ctgggtcgtt ggaaacgaga accttgggga
       61 gccggagagg tcttggtgaa gagtatcgtg aatgattatg tgtctgtgct cattaccaat
      121 cgacgggctt tgggattttc tgcattgacc ggagggtttt ttccgcaaga tcttccatcc
      181 ggcaatgtga ttgaccagac ggaagccaat gcgaatattg tgatcatcca tctgtcagga
      241 ctgatgttgg tgttccgatc cggtttggcg atttggacgg tgttgccctg attaattgga
      301 ggcaggacta ctagccaatt cctggtgttt ggtcacgcct ggataatcca ccccaatagg
      361 gctgtttgag caataatggc tccaccagtc aatgacttcc tgaagaaaag gatcttccgg
      421 ccctgtcgca aacaggtgac catagagata gccggtttgg gtatcaaaga ggatgaatat
      481 cggcatgggg gcaggttgtg gaagatgtgg cgtgtccatg ggtcgtcggg atcgtgcgtc
      541 agaggttcct ttcgtttttc tctaggtatt cttgtcggaa cgtgttggat atttcttaaa
      601 tttagccttg gatcaaaggg ggttgatcgt tcatgggaaa ttcagggtct cttcggggtt
      661 ttacaaggcg aggggaaggg acccgacaac gattggggca aattagaaga caacccttga
      721 ccttccccgt caaatggggt agctatgttc ctttaccgca caaccgggtc aatggtaagg
      781 gcgattatct gggctgagag ggaggctaat catggatgcg ttcgattctg ctggatcagc
      841 tatcacgaag caggttgcca ggatcctgct ggttgaagat gaagtcgatg tgcgggagtc
      901 catcaagttg cagcttgagg atgagggaca tcaggttgtg gacacggaat ccggcggcga
      961 cgccctgatt ctggccgagt cctctcagtt cgatattgtc ctgaccgacg tgatgattcc
     1021 cgacatcaac ggaattgagc tggtccagcg cattacgcag cttcccaatc atccggtcat
     1081 gatcgtcatg acgggatatg ggtcggtgga gatggcggtg aaagcgatta aggccggtgc
     1141 ctttgatttt ctctctaaac ccttttccat ggatgtgctg agtgccacgc ttgccagtgc
     1201 cttacgcgtc aagtgtcttc aggacgagaa tgtcgaattg aaaaaaaccg tcaagggcca
     1261 atttagttta ggaaaactca ttagctccag tcaggtgatg caagaagtgt ttcagctgat
     1321 cgattgcgtg gcggataccg atagtaccgt gctgatttta ggagaaagtg gaaccggcaa
     1381 agaattaatt gcccaaacca ttcattgtca tagcacgcgg cgtcagggaa gcctgattcc
     1441 tgtcaattgc ggagcgattc ccgaggcgtt attagaaagt gaattgttcg gtcatgagaa
     1501 aggtgccttt accggagcgg tcgcatcacg agtcggacga tttgagttgg cgaccggagg
     1561 cacaattttt cttgatgaaa tcggggatct gagtcctccg ctccaagtca aacttcttcg
     1621 tgtcctacag gagcgaacgt ttgaacgggt agggggtact cgaacgtata aatcggatgc
     1681 tcgtgtcatt gccgccacga atcaagacct tgaaaccctg gtggctgcga aacaattccg
     1741 ggaagatttg ttttatcggt taaacgtggt gccggttctt gtccctcctc tccggcatcg
     1801 ggtcgaagat atcccgctat tgctccagca tttcatcacg atgttcaatg atcgccggaa
     1861 agcggaactc accggagtgg ctgatgatgc gatgagtctc ttatgtgaat atccctggcc
     1921 gggaaatgtt cgtgaactcg gaaatatcgt tgagcggatt gcgatcttga aacggcgggg
     1981 gttgatcgtc ccggaagatt tgccggaaaa aatccggcga ttaccctcct ctcatctccc
     2041 caccatgccc gcggcaattc ccgtggccgg gatggatctc tcaagaacgg ttgaggagtt
     2101 tgaaaaccgg ttgattcttg aagcgctcga gcgaacgaat tgggtcaaaa gtaaagcggc
     2161 tcgtctcctg caaatcaatc gcaccaccct cattgaaaag ttaaaaaaga aatccctggc
     2221 cgaagtggtc gcacaacgtg cgaccccatc ggaagatctg gccgacgtgt aacaatcgag
     2281 gacgccccgc ctttcctacc ctgtttcgcc ttcccatttc gaacaagcct gaggttctga
     2341 ccattccgtc aactccttga cggcggcaat attgtccgtt cgtcttcgtc cttgcaaaac
     2401 ttcctggttt ttcaacgaaa accctgttgt cgattttttg ccatcggcag gaatgactcc
     2461 ggtctaaatt ttgctgaaga ggtctatcag gttggagcag aaaccaaaag tgaggaaatt
     2521 tacttggatt gccctgttgt gtgttttctg gcccagcgca gttttgggag gcccgtgttc
     2581 tgatttttcc tggtacgttt cccgcgacat ggttatgccg gagattcttc gacagggaca
     2641 ggatgcctta cgcgcgggac aactcttgga agccagagat atgtttgcca cctatttaaa
     2701 agagcaggag catgggcagt ttgcagaagg gacccgttgg gtcctggcct ctcttcctga
     2761 tcctctggat gaaccaggta aagaaaaatt tcaacgcatc gagcgtctgc aagcgatgaa
     2821 aatgagtgat ccggagagta tgtatgtccc atgggccgtg tgtgccatgg gtaatgtgta
     2881 ttgggaggct ggatggtttt cagaagccag cggaatattc gaagattttc tccggtccta
     2941 tcccgatcat ccgttagcgg gaggagtcat ggtcgaagcc gggctagggt atctgagcaa
     3001 taagcaatat ttggaggcag cactgattct cagacgagtg gtagaagaac ccaagtggag
     3061 tggccatcgc ctgaaggctg cattaggttt ggcggatgcc acggccatgg cgaaggcgtg
     3121 gaagcaagcc tactactggt accgggtggt cgaagccgaa aagcctgaac tcattcgccg
     3181 gtctgggaat tcagcgtatc actatggatt aacggaactt gccgtcggca atcccgccat
     3241 ggcgatatcc agatttttgt tgacggtgaa tcttcatccg gatgaggaac cagccgggat
     3301 ggccttgagc caaatatcag aaaaattacg gaaagacgga catgaatatc tgggcttgta
     3361 ttttgccgat caagcccgac aacgttttcc cgaccaggaa cccggtcgac gtggacaggc
     3421 cgcgctgacc agatgggtcg tggcgtttgt cacccaggag cacgccaagg aggactgggt
     3481 caaggtgtat catcggtttg atgatttgga aatctatctc tcgctctcat gggattatgt
     3541 tctagaaacg gccggtatgc tcagtcatgc cctggaaagg gatgtggctg atgagagcct
     3601 gctgtggatg gggcaagcct atcaggcgct aggggaatat gctgatgcgg ttcaaaccta
     3661 tacccgcctc attgtcgtga cctcgtccga tgagaggcgt cagacaggac aggatcgact
     3721 cgcccgcttg ttacagcatc agatgcgttc cttctatgat tcgaaagcct gggtgccgtt
     3781 actcaagttt cacgacgact atcaagaggc gtttcagctg gtgcccttgg aacgggagcg
     3841 tctcttcatg gtggcgcaag cctatcaaca agtgaaactt ccggcagaag cctggcattg
     3901 gtatggccaa ctcttaaagg agtaccccga cagtccgctt cgggaagaca ttcgtcttca
     3961 gcaggtggtg gttgctcaag agcaggaaaa cacatcttgg gtgcgagagg cgggagcgtc
     4021 ctatctccgg gagtttccca atggccaggg acgagggcag gtggagacca ttcttgcctt
     4081 ggaagccctc acggacaaac gttatgcaga agcgatacac ggtttctccg cggctctcca
     4141 gcatgtggac ggtgaagtag agcaacgcta tgtgctccgg aatcgggcac gcgcctatcg
     4201 agcgctcggg cagatggagt ccgtggtgca ggacctccga caggtggtgg ctattcaacc
     4261 cacgcaggtt tccgatgtcc tccgtttggg ggatgccctg tttgatcatg gggactatgt
     4321 cgaagctcag catctgtacg atcaagccct ggcctatgat gcgccgccgg ccttgcacac
     4381 ctgggccaaa tatcggttag cggcatcgct tgaatacatg gggcagtctg atgaagcggc
     4441 cgcactgctc gctgaaatcc gtcaattaca aacccgttct ccggaattgg aacatacgat
     4501 tcgctcagcc gccacggcgg tgttggatga aatgtcctcg aaagaaacgc cattaaccag
     4561 gatacccgat gtcccaattc aatcatagac cgatgatcgc gttgagttcc gagaggaata
     4621 aggaattgaa ctggccgaca ctcgacgcta ccaccgagcc aacgatctcc agtgaaaccc
     4681 tcagtctggc gctagaggcc aaatatgcgg aaacgcaacg attgcatcat catctacatc
     4741 gtttggtgaa aagtcttccg ttacccgcct tgctctataa ccgtaaaggt cggattgtga
     4801 cagccaatca cgaggcccat gtgctgatgg gaggaatcga ttggaaaaag gtgaactggc
     4861 gggttcagga tctttggaca agcctgggat ggccatcggt tccattttca gactggaagt
     4921 ggaagtcagg ccgggtgtcc tgttgggatt gtccgttagg aggtatggat caaccgtcca
     4981 gtctgacggt gcgttatctc aaatacgaag gggatgcgca aagtggacgg acggaacaga
     5041 ttgaacggtt ggcgaccata ggagaacagg ttgggcggct ggcgcatgat attcggaatc
     5101 cattggcgag tattgaatgg tttgccacgc ttttggggcg ggacagtccg tccggcaagg
     5161 gacgtcggga gctggcggat caattacttc atgccattcg ttcattggag ggactggttt
     5221 cgaatctctt aacatccaca accccattga agggaagccg gcagtgcgtg aacctcctgg
     5281 aactgttgga tgatgtggag ctcctggcca cgtttcccct cagaatcaaa cggctgacca
     5341 ttcaccggca gcgagaaacc tcacttctcg agatcttggg tcatgagcag ttattaaaac
     5401 aggcgctctt gaatctgctc ttgaatgcga ttcaggcttc tccgcctgat ggtcatattg
     5461 agattcagtg tcgacgaatt tctcggccgg ctccgggagg ggccgcctcc gccggggtca
     5521 acggcgtggc agtgaatatt cgagatgaag gatgtggcat gtccgaagaa gaactcacca
     5581 gggtctttca gccattttat tcgaaacgtc agggagggac cggcctggga ctcccgatcg
     5641 tgaaagacat tatgcaggtg catcaaggca tgattgagat ggaaagccag accggcaaag
     5701 gaactacggt caaactattt tttccccaat aaaggagatt ggcatgatcg atactgaggg
     5761 tggagtgggc cggggtattc tggtcgttga agatgatgaa cagttgcaat cggccctgac
     5821 ttacgcgttg agtcagcatg ggtatgccgt cactgttgcc agagatggct acgagggatt
     5881 ggagcggatg gaacgcgagg ccccatggct ggtgttgact gatctgaatt tgcccggcaa
     5941 aggagggatg gattttttac gagaagtccg aagccagggc taccatatgc cggtggtggt
     6001 tatgaccgcc tatggtggag tggatgcagc cgtcgaggct ctcaaatatg gagcaaccga
     6061 ttttcttcaa aagccatttc cccttgatcg ccttgaaaaa ttaatagacc agttaaaaga
     6121 tgcgcagcct gtgtcaggac tggaggttct gagggaacag gaacgatgtc gccgtgaaga
     6181 gccgcgtgaa ggatttctga ccagagatcc gaaagtgttg aacactatca gcatgttgga
     6241 gatggtggcg gccagccctg cgaccatttt gattcaaggc gaaagcggga cgggaaagga
     6301 agtcctggcc cggcatgtcc atcgtcatag tcccagggcc tctcaaccct ttgtggcaat
     6361 caattgtgcc gctctgccgg aaggactctt ggaaagtgaa ttgtttggct atgaaaaggg
     6421 ggcctttacc ggcgcgctcg cgagacgatg cggcaagttt gaattagctc accagggaac
     6481 gttgttgttg gatgaaatcg gggaaatgag tttgggatta caggccaaat tactcagggt
     6541 ccttcaagaa cgtgaagtgg atcgccttgg ctccagacag ccggtgcagg tggatgtgcg
     6601 cgtcattgcc acgacgaacc ggaatctggc gcaggaagtt caggccggac ggtttcgtga
     6661 ggatgtgtat taccggctga gtgtcatgcc gtttactatc ccccccttgc gtgatcgccc
     6721 ggaggacgtt caattattgg cggaatattt tgccaaccgc tcattgcggc gaaaccggcg
     6781 cacgccgtgc gagatctctg aggaggcgat gtcctatttg aaacggcggc cgtggcgggg
     6841 aaacgtacgc gaactggaaa atgtgattga acggggcgtg ttgttagccg gcaatgggcc
     6901 attactgatt gagcattttc agtttgagga acccgtgttg gcggccaatc atgccagtgc
     6961 gccaagtggg actatctggg aaatggaacg ggaacttatt ttgagaactc tggagcgcca
     7021 tgatgggaat cggacccacg cggccaggac cctgggaatc agtattcgta ccttacggaa
     7081 taaattacgg gaatatcgac agttaaacgg ggggctttcc ctgggtattt aagagaaggc
     7141 aatgggaaga acatctgaat gcctcgcttc tgccgggtgt gaggaacagc agaaggaggg
     7201 cggaaaaata tgccggtgcg ggcaaaagtt tccttgaggc cattaacgac gtacggtgag
     7261 agagttggtg agtgagggag aaagcgtgag ggtctcgttt ttcgccttac ggttcattgc
     7321 ttaccggtaa tttctggcat tggtgttgct cccttgcgtg aggacgtgag ggtgttcgcg
     7381 gaccggccgg atgggggggc aggtgcagat acaggagaac atgaaaaccg gaaaggagag
     7441 aaggccatga tgatttcacc gtgggatgaa ggcacgcgac tctttgagcg gtcactggat
     7501 gtccggagcg gcatccagga aacgattgcc tccaacatgg ccaacgaaga aacgccgggc
     7561 tataagtcca ggatcctccc tttccaagaa accttcaatg cggttcttcg aggggatggt
     7621 ccattggagt cagtcgtcac gaatccgaaa catttgcgca ctgcctcaga ggattcccgg
     7681 atatttcagc acaccatcaa acaggatgcc ggagccgggt tagacgagaa taccgtgaat
     7741 ttggaaaaag aaatgaccct gatggcggaa aataccttga tgtatatggc ggtcagtcaa
     7801 tttttaaaag ggcgcttcga tgggtggaat gcggccattg atggaggtag tggcggtagg
     7861 taaacgcctt tgagcgcatg gttcccatta cgctgatccc aggaaagccg agagaacgag
     7921 tgaagcggga agttggtaaa gatgagatgg atgggaagat ctgaattggg tgtgggaata
     7981 aggaggttac gatgaatatt gatcgagtgt tttcggtcgt ggggtctgcc ttggatgcac
     8041 aacggcagcg gctgaatatc attgccagca atttggccaa tgcggaatcc acccggacgc
     8101 cggaaggggg accatacatc agacgtgatg tcgtgtttca agcgtcaccg gcagggccgc
     8161 agttttccaa tgtgtttgcc aatgcctttg gcgatcagtc cggaccatct ggcgttcggg
     8221 tgacggatgt gatccaagac gagcgaccgt taaggaccgt gtacgatccc agtcatcccg
     8281 atgcggatga acaggggtat gtacaccttc cgaatgtgaa cccgatcgaa gagatggtga
     8341 atctcatgtc ggcgacccgg gcctacgagg ccaatctagc cgtgatggaa accggcaaga
     8401 ctatgacgct tcgtgcatta gaaatgagtc gctaaggctc ttcaatcctg aaggaaggag
     8461 aacggcaaaa tggactatct tccgataaaa agcattgagg gtgtttcgga accgtttggg
     8521 gtcaccccgc tcaaaggccc agccggtcag gaccagggat tcggcgatct cttgaaaaag
     8581 gcggtggagt ccgtcaatgc catgcagcat gaggccgggc gtttagaaga tgccgtcgct
     8641 cgtggagaga acgtcaacat tcatcaagcg gtcattgcgg gtgaaaaagc cggcttatcg
     8701 ttcaaactct tgatgcaggt cagaaataag gtgattgatg cgtatcagga agttatgcgg
     8761 atgcaagtgt aagacatggt tgcgttgaca cacgctgatg ccgaccatgg cggaggcccg
     8821 aatgagagag agccggcacc ggtcacatcc cgcacgactc atcggtgtaa aaatgatgcc
     8881 cacatttctt caattgtgag tgtgagaaga gccggttaat cctatggaca gtcttctcag
     8941 taattttcaa gccttaacct taacgcagcg gatcggcgtg gtcgtcgtcc tggccttagc
     9001 cctggcgacc attccgatgt tgatgatggt cggaagggcc cctgaattgg tggttttgtt
     9061 ttctcagctc aatcccgacg atacgcgagc gattatccag gaacttggga agcaaggcgc
     9121 tgtctatgag gtgggtgaag gcgggaatac gattaaagtt ccagccgaac gagtgcatga
     9181 attgcgtctc cagctggcct cgttaggact tcctgaatcg gctggagtgg gatttgaaat
     9241 attcgatcga accgggttgg gggtgacgcc ttttacgcaa caaatgaatt atcggcgtgc
     9301 gcttcaagga gaactggctc ggaccatcgg gcaattatcc caggttgagc gggtacgcgt
     9361 gcatctggtg atgcctgaga agcgtctgtt cgcgacggaa caaaaaccag cccaggctgc
     9421 cgtcgttttg acgctcaagc gtggggctcc tcttggtggc acgcaggttc aggggatcgt
     9481 gcatttagtg gccagtagcg tcgaaggatt agagcccggc caggtgacgg tcgtggacaa
     9541 tcacggtcag gtgctcagtc aaaactctgc ggatagtgac gctcaactga cggcttctca
     9601 aattgaaact caacgacggg tagaacggga tctggagcaa cgggtgcaga ccatgttgga
     9661 ccaggtcctg ggccgggaca agtctgtgat tcgggttacc gcgccgctgg agtttcggca
     9721 agtcgaaatc accgaagaaa gctttgaccc caatagccag gtggtgcgaa gcgaaaaccg
     9781 gagtcaggag aaggtgctgg aatccggttc gacccacggg ggaccgggtg ttcctggtgt
     9841 gaggagcaat gtgcccagcg agctcaaggc aggaaacgga acagagcaaa aagaagccaa
     9901 acgaaaaaat gaaacattga attatgaagt caatcgtaaa gtgagcaaaa tcattgaacc
     9961 gaccggcgcc attaagcggt taagcgtggc ggtcttagtg gatgggacct atgaagcggc
    10021 tcaggacgct gaggggcagg ggactgggag tacctccgaa aagaaatatg tgccacggcc
    10081 tgaacaggaa atccagaatc tggtccaaat tgtgaaaaaa gcggtcggat tctccgaaga
    10141 acgcggggat caaatcgaag tcatcaacgt gccatttgaa tctcccgcca tccttgaagg
    10201 tgaggagagt ctgaccgcag gtgtgcaatc gtttctggcc acctggggtg gatttctgaa
    10261 gcccgttctc tttttcttcc tgggagtgat ggtgttgtgg tttgtcgtac gaccgatggc
    10321 cctgaatctc acgaagcccg ctgcggcagc ggtggtcctg ccacagaaag gattgccggc
    10381 cacggtgacc gaatatgaag ccgaaatttc cgaaacccca caggagcaag ccattaagct
    10441 ggccgcagat aatccggctt ccgccgcgca agtgattcga acctggatta aagaagaaca
    10501 aggagaaaag gtttaatccg tgaatagcca gttgtccgga tatgaaaagg ccgcgatttt
    10561 gttgcttgcc attggggaaa gtgctgcggt ggaagtcttg aaagtgctcg atcaaaagga
    10621 cattcggcaa atcggcgcgt atttgggggc gttggtcaat gtcgggcagg acgaacatcg
    10681 attggtgtta aaagaattcc gggaattggc cggaacatcg ggtctgtcgg tggaaggcaa
    10741 aacctatctt tcaaaaattc tcaatgcggc gctgggaaag gataaagccc gtcgaattct
    10801 gagttccctg aacacgtcgg agaatgccgg atttgaaacc ttaaaatccc tcgatgcggc
    10861 atcgattgcg aacttattgg ccattgagca tcctcaaaca ggcgccttga ttctggccaa
    10921 tttagaatcc gatcacgcgg ctcagatttt gtcattgctc cccgcaaata aacgggatgc
    10981 catcgtctac cggttggcta cgacagaaga agtctcgccc aatatgttgg acgaattgaa
    11041 tgctgtgctt caggaggaat tgcgtctggg ttcttccaaa aatgcggtgg ccgtgggcgg
    11101 gactgggctg gttgccgaaa tcctgaattc tgtgaaacgc
//
LOCUS       DATIAL010000513        11725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_199247, whole genome shotgun sequence.
ACCESSION   DATIAL010000513 DATIAL010000000
VERSION     DATIAL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11725
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199247"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VLA60_15735"
     CDS             <1..803
                     /locus_tag="VLA60_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF10863.1"
                     /translation="PLEKLLSTQHQEIAGLILEPMVQAVAGIIPSPPGYLKRVRELCT
                     QYKVLFIADEVATGFGRTGRMFACEHEGISPDIMAIAKGLTGGYLPLAATLTTNAIYE
                     AFLGEGHENKTFFHGHSYAGNPLGCAAALANLAIFQSERTLAKLQRRIPKFRRALDSL
                     TEDPWVGDIRQCGFMVGIELVQQKSQKMPFPASERIGQKIAMTARALGLLIRPIGTIM
                     ILIPPLSASAKELDQMVSILKLAISVVRASSTSFPISSPPEVLKHTSD"
     gene            complement(800..1600)
                     /locus_tag="VLA60_15740"
     CDS             complement(800..1600)
                     /locus_tag="VLA60_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HSF10864.1"
                     /translation="MTSAISLVTLVTDFGDVDYFVPSMKGVMLGINSQIRIVDLTHRI
                     PAHGVEQAAFFLKSCYQYFPDGTVHVVVVDPGVGSSRRAILASTSRHFFLAPDNGVLT
                     YVLQEETAVQVRSIENKQYRLDSIGGTFDGRDLFAPSAAWLTKGQVPGSYGRLIHDYV
                     RLPLDPPRMEGHALHGRIVYIDHFGNAITNVTLTDIETFRSVTKKECSGLKIGEVIIK
                     GIKTHYEEGALGYPEGLINSNGHIEIFVKQGRAVDRCQLHIGQTVELI"
     gene            complement(1603..2079)
                     /gene="greA"
                     /locus_tag="VLA60_15745"
     CDS             complement(1603..2079)
                     /gene="greA"
                     /locus_tag="VLA60_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HSF10865.1"
                     /translation="MKIPMTKKGYEALQAELARLRREDRPKNIQAITEAREHGDLKEN
                     AEYKAAKEQQQFIDTRMSELEHKLSIAQVVEISPGQSDTVVFGATVTILSLESQEEKR
                     YTLVGQEEADIKNGSISVQAPIGRALIGHRVGDIVEVHRPAGVIEYQIQSICFEEL"
     gene            complement(2154..5456)
                     /gene="carB"
                     /locus_tag="VLA60_15750"
     CDS             complement(2154..5456)
                     /gene="carB"
                     /locus_tag="VLA60_15750"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HSF10866.1"
                     /translation="MPRRNDIHRILLIGSGPIVIGQGCEFDYSGTQACRALKEEGFEV
                     ILVNSNPATIMTDPDLADRTYVEPITVEVVEAILERERPDALLPTMGGQTALNITVEL
                     VKRGILEKHGVQLIGASYESIQKAEDRDLFKQAMTRIGLNVAASGSFRSREEAVRLLE
                     TIGFPAIVRPSFTLGGAGGNIAYNREEFDKYIDWALVTSPVGQALLEQSLLGWKEYEL
                     EVMRDSKDNVVIVCPIENFDPMGVHTGDSITVAPAMTLSDKEYQHMRDAAIRIIREIG
                     VDTGGSNIQFSLNPDNGALMVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITQVTKACFEPTIDYVVVKIPRFTFEKFDGVDRVLTTHMKSVGEAMALGSTFKE
                     ALQKAIRSLETDRYGLMSLHGLNGKKDSAGSAHPLYERLQSQVRIPTADRLWFLADAF
                     RIGMSVEELYHLSRVDPWFLHEIEELVEFEAEWVCRSKAEVGNDRSHDTRKGCPASLV
                     TLLEQAKSLGFSDQRLGQLIGREEDTIRQWRQVRGSGYGTVFKRVDTCGAEFEAHTPY
                     LYSTTGFSCESRPTQKRKIMILGGGPNRIGQGIEFDYCCVHAAMALKEEGIETIMVNC
                     NPETVSTDYDISDRLYFEPLTKEEVLRIILTERPDGVVVQFGGQTPLKLAVPLEQEGV
                     RILGTQPDAIDRAEDRKRFSDLLFELNLLQPQNGTARSSHEAHIIAVNIGYPVMVRPS
                     YVLGGRAMQIVYDADALDEYLQRHEMDLGRHPLLIDDYLEDAIEVDVDAIADGTDVVV
                     AAIMEHVEMAGIHSGDSACSLPAHSLNADILKTINHQTILLAKELHVVGLMNIQYAVK
                     DGAVYILEVNPRASRTVPFVSKAIGVPLAKHAMKIMAGRTLKDLGFTQCPLFSHIAIK
                     EAVFPFTKLGVADVLLGPEMRSTGEVMGIDRSFGWAFAKSQAASGMPLPLAGTALLSV
                     KDGDKPATLAIAQRLSQLGFSLNATKGTAAFLRTHGITVVTVNKINEARPHIEDLLKN
                     NELSLVVNTVGGASSQDDSAPIRRASVFGGIPYFTTIQAAQAAISGIEAMKKTSMEVR
                     CLQEYHAAMPGR"
     gene            complement(5466..6362)
                     /locus_tag="VLA60_15755"
     CDS             complement(5466..6362)
                     /locus_tag="VLA60_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSF10867.1"
                     /translation="MINDPSGSGMTFQWLLASAVCALLLLGSACQKAPGTARDQLIYI
                     SEEKEIALGLAAFREVLKSAPLSRDPEIALMVNRVGQRIAKAAQKPEYVWEFAVIQDD
                     DQVNAFALPGGKVAVFTGILKYTKTEAGLATVMAHEVAHALQRHGAERYSRGILEQIG
                     QLGALAGAAAGGVDPGLAIGAMSAYGVGVALPNSRDQETEADFIGLQLMAKAGYDPRE
                     AVPFWERMSGCPRKMIGKFCFRSQNAIPEFLSTHPSDVTRINQIEAWIPQALKFYHPE
                     TSPPESPESPAKKPISGSVLQS"
     gene            complement(6399..7355)
                     /gene="sppA"
                     /locus_tag="VLA60_15760"
     CDS             complement(6399..7355)
                     /gene="sppA"
                     /locus_tag="VLA60_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HSF10868.1"
                     /translation="MTRCYLILFLFLVSGCIHVDLFPGGGKLQEAIISGEGKDKVLLI
                     DISGMLTTGKASGILEEPSLPARIKEELTKAEEDKHVKAIILRINTPGGSVTASDLVY
                     HELKVFKKKRVVPVIAAIMDLGTSGGYYIAMSADHILAHPSTITGSIGVIMVTMNAEG
                     LLEKIGVQPAAIVSGPKKAMGSPFRPMNDEERAIFQGTIDHLFEQFLSVVKEGRPGLS
                     MEQIRTLADGRIFTADIAKSKGLVDSIGYLDEAIDLAKKEANLEQAKVVTYTRGRGTH
                     QNIYSRFDPPQIGPIGFPQVDAHSLFNVLTGGTPQMLYMWMP"
     gene            complement(7361..8545)
                     /gene="carA"
                     /locus_tag="VLA60_15765"
     CDS             complement(7361..8545)
                     /gene="carA"
                     /locus_tag="VLA60_15765"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HSF10869.1"
                     /translation="MKPAVLALADGTIFQGRALGFEGETEGEVVFNTAMTGYQEILTD
                     PSYKGQIVLMTSTQIGNYGVTPEDNESHRVWAEGFIVREAAKYWSNWRSRQSFDDYLI
                     EHRIVAIQGIDTRALTCHVRDHGSQMGIISHVDFNDNGLREKAKSLPSLVGRDLVREV
                     TCPDAYEWTERSIVMDPASGDPSIFTQNTAQPPLKLVVMDFGVKQNILRSLVDLGCQV
                     RVVPASTTAEEVRRLNPDGIVLSNGPGDPEGVPYAVETVKQLLGWKPLLGICLGHQVL
                     GLSLGLHTHKLTFGHHGANHPVMDLRTRKIEITSQNHNFAVGLPHKEDVCTTLTSQQF
                     DSCVGRMEITHRSVNDGCCEGMAGLESPILSIQYHPEASPGPHDSSYVFRQFLEMMKG
                     PG"
     gene            complement(8594..9124)
                     /locus_tag="VLA60_15770"
     CDS             complement(8594..9124)
                     /locus_tag="VLA60_15770"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HSF10870.1"
                     /translation="MKRINWGVVGFLFELCALILWVGGLVVIIALVIPAVFNSFGMEP
                     AGRFLRRVFDGFGLMNAWILILLSVVAVIRFRAFGHNPSEMFAVSSVEWCLLAGMALM
                     TFSILVVLGPQAITLQEEAFDAVSKEDKDIAYAKFFRLHMVVRACHLVNFGLAASLFI
                     VKVRKALFHDTITPRS"
     gene            complement(9134..10456)
                     /locus_tag="VLA60_15775"
     CDS             complement(9134..10456)
                     /locus_tag="VLA60_15775"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HSF10871.1"
                     /translation="MTKKTGLSPTSSHLLCIQGGIVIDPGHVNGRADVLIQDGNILEI
                     GFPLTNPLCQDSSVTILEANGWIVAPGLVDLHCHLREPGFEYKETIATGAASAVAGGF
                     TTICCMPNTQPVNDNAAITKFMLAQGQEAGKARVFPIGAITKKSEGEELADIGELVDA
                     GCVAISDDGRPVMNSLVMRRALEYAKAFGIPVVDHCEDLHLTDGGCMNEGMVSTELGM
                     PGIPDASEEVMVARNLALADLTGVHVHLAHLSTARSVELVRHAKGQGLPVSAEVCPHH
                     FSITEEAVRCYNSNAKMNPPLRTDEDVQALKQGLVDGTIDAIATDHAPHALQEKQLEF
                     DTAPFGIVGFETALPLTLRLVHEGVLSLEQALDKLTRSPAQLFHLPVGSLQPGTHADI
                     VVFDPHEEWVVDPTKFFSKSQNTPFGGWTVKGKVKMTLVDGRMVYDAR"
     gene            complement(10466..11407)
                     /locus_tag="VLA60_15780"
     CDS             complement(10466..11407)
                     /locus_tag="VLA60_15780"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HSF10872.1"
                     /translation="MGLERKDLLTIAALSADQIQLILTTAESLKEVGGRDIKKVPALR
                     GKTVVNLFFEPSTRTRTSFELAAKRLSADVINFSPSTSSMVKGETLVDTARNIEAMQA
                     DIIVLRHPSPGAAENLARSVRSSVINAGDGWHEHPTQALLDVFTIKEKKGRVSGLVVV
                     IVGDIAHSRVARSNILALTKLGAEVRVVAPPTMIPFGLEHFGVEVFYDLDEALIGTDV
                     IIMLRLQRERLGRALLPSLREYAKLFCLTPERLKLAKPDAIVMHPGPLNRGVEISPSV
                     ADSNVAVILDQVTNGVSVRMALMYLLSGSSERSTLNE"
     gene            complement(11415..>11725)
                     /gene="pyrR"
                     /locus_tag="VLA60_15785"
     CDS             complement(11415..>11725)
                     /gene="pyrR"
                     /locus_tag="VLA60_15785"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HSF10873.1"
                     /translation="SIRKDQPEIRKTTIPFHISDLKIVLVDDVLFTGRTIRAALDGLM
                     DLGRPAEIQLAVLVDRGHRQLPIRANYVGKSIPTAREENVQVFFEELGQDDRVSILTP
                     "
BASE COUNT         2984 a         3141 c         2653 g         2947 t
ORIGIN      
        1 atccgcttga aaaacttctt tccacacagc atcaagaaat tgcaggtctc attcttgagc
       61 ccatggtgca agcggtcgcc ggaatcattc cctcgcctcc aggatatttg aaacgcgtcc
      121 gggagttatg cacgcagtac aaggtcctat tcattgccga tgaggttgcc accgggtttg
      181 gacgaacagg tcggatgttt gcttgtgagc atgaaggcat ttcaccggat atcatggcta
      241 ttgccaaagg tttaacggga ggctatctcc cattggccgc cacattgacg accaacgcca
      301 tttatgaagc gtttttggga gaagggcacg aaaacaaaac attttttcat ggacatagtt
      361 atgcggggaa tcccttggga tgcgcggcgg ctctggccaa ccttgccatc tttcagagtg
      421 aacgaaccct cgcaaagctt caacgacgga tccctaaatt tcgcagagct cttgactccc
      481 tgaccgaaga cccttgggtt ggggatatcc ggcaatgtgg ctttatggtt ggaattgaat
      541 tagttcaaca aaaatcccaa aaaatgccgt tcccggcgtc cgaacgtatt ggccagaaaa
      601 tagccatgac cgccagagca ttaggactgc tgatccgtcc catcgggacc attatgattc
      661 tcattccgcc tctttcggca agtgccaagg aactcgatca aatggtgtct attttaaaac
      721 tggccatttc agtggtccgt gcgtccagca catctttccc aatatcatca cctccggaag
      781 tattgaagca tacctcggac tagatgagct caaccgtctg gccaatgtgc aattgacatc
      841 ggtccacagc ccgtccttgc ttcacaaaaa tctcgatatg cccattacta tttatgagcc
      901 cttccgggta tcccaaggca ccttcttcgt aatgagtttt tattcccttg atgatgacct
      961 ctccaatttt gagtccggag cattctttct tggtcactga tcgaaacgtc tctatgtcgg
     1021 tcagggtcac attcgttatg gcattgccaa aatggtcgat atagacaatt cgcccatgta
     1081 gggcatgtcc ttccatacgt gggggatcaa ggggaagtct gacataatca tgaatgagac
     1141 gcccgtacga accgggaacc tgccccttcg tcaaccacgc tgccgaaggc gcaaataaat
     1201 cccgtccatc aaacgtccct ccaatggagt ccaatcgata ttgtttattc tcaattgatc
     1261 ggacttgaac cgcggtttcc tcttgaagga cgtaggtcaa gaccccatta tccggagcca
     1321 ggaaaaaatg cctggaagtt gaggccaaaa tcgctcttcg agagctcccc accccagggt
     1381 ctacgaccac cacatgcacc gttccatctg gaaaatattg atagcatgat ttcaaaaaaa
     1441 atgccgcttg ttcaactcca tgcgccggaa ttcgatgcgt cagatcgacg atgcgtattt
     1501 gagaattaat tccgagcatc acgcccttca tacttggaac aaaataatcg acatccccaa
     1561 aatcagtgac caacgtaacc aaggaaatgg cggaagtcat gattatagct cctcaaaaca
     1621 aatggactgg atttgatact caataactcc ggcgggtcga tgcacctcca caatatcccc
     1681 gacccgatgc ccgatcagcg cacgcccaat aggtgcttga actgaaatcg aaccattttt
     1741 gatatcggcc tcttcttgtc ccaccaaggt ataacgtttt tcttcttgtg actctaaact
     1801 taaaatggtg actgtggccc caaacacgac cgtgtcactc tgcccgggcg aaatttccac
     1861 cacttgagca atactaagtt tatgctcaag ctcagacatc cgtgtatcaa taaattgttg
     1921 ttgctccttg gcggctttat attcagcatt ttccttgaga tcaccatgtt cacgagcttc
     1981 ggtgatagct tggatgtttt tagggcgatc ttcccgccgc agcctggcca gttctgcttg
     2041 cagtgcctca tacccttttt tggtcatagg aattttcatg aaacctcact cccctctcta
     2101 aatcatggaa atcggatgtc aatcatccaa catcgcgtcc cgtccactta ggcctacctg
     2161 cctggcatcg cagcatgata ttcctgcaga cagcgaacct ccatggatgt tttcttcatc
     2221 gcctcaattc ctgagatggc ggcttgcgca gcctgaatgg tcgtaaaata aggaattccg
     2281 ccgaagacgg acgccctgcg tatcggcgcg gaatcatcct gggaagaggc acctcccacc
     2341 gtattgacga ctaatgacag ctcgttattt ttgagtagat cttcgatatg gggccgagcc
     2401 tcattgattt tattcaccgt aaccacggtg atcccatgtg tccgcaaaaa ggctgccgtc
     2461 cctttcgtgg cattcagtga aaatccgagc tgcgacagtc gttgtgcgat agcaagcgtc
     2521 gcgggtttat ctccatcttt cacgctgaga agggccgtcc ctgccaaagg caatggcatt
     2581 cccgatgcgg cctgtgattt ggcaaacgcc caaccgaagc ttcgatcaat ccccatgact
     2641 tcgcccgtcg aacgcatctc gggccccaac aatacatcgg cgacgccaag cttggtaaat
     2701 ggaaagacgg cctctttaat ggcaatgtgg gaaaaaagtg ggcactgcgt aaagcctaag
     2761 tctttcaagg ttcgccctgc catgattttc atcgcatgtt tagccaaagg cacaccgatg
     2821 gccttactga caaaagggac cgtgcgggaa gcgcgaggat tgacctccaa gatatacacc
     2881 gccccatcct tgactgcata ttgaatattc atcaggccca ctacgtgtaa ttccttggca
     2941 agaagtatgg tttggtgatt gatcgtcttc agaatatcgg cattaaggga atgggcaggt
     3001 aatgaacaag ccgaatctcc tgaatgaatg cctgccatct cgacatgttc catgatggca
     3061 gccaccacga cgtctgttcc atctgctatg gcatccacat caacttctat ggcgtcttca
     3121 aggtaatcat caattaacaa gggatgtctt cccaaatcca tctcgtgtct ctgaaggtat
     3181 tcatctaatg cgtcggcatc atacacgatt tgcatggcgc gcccccccaa cacatacgat
     3241 gggcggacca tcactggata accaatgttg acggctataa tatgagcttc atgtgaggac
     3301 cgtgcggtcc cgttttgcgg ctgcaggagg ttcaattcaa ataacaaatc actgaatcgt
     3361 tttcgatctt ccgcacggtc aatggcatca ggttgtgtcc ccaaaattct gaccccttcc
     3421 tgctctaacg ggacggcaag cttgagaggc gtttgcccac cgaattgcac gacgactccg
     3481 tccggtcgtt cagtcaggat aattcgcagg acctcttctt tggtcaacgg ttcaaaatac
     3541 aggcgatcgg aaatatcata atccgtactg acggtttccg gattgcaatt gaccataatc
     3601 gtttcaatgc cttcttcttt tagggccatt gcggcatgca cacaacaata atcaaattca
     3661 atgccctgcc caatccggtt cggtcctccg cctaagatca taatttttct tttttgagta
     3721 ggacgggatt cacaggagaa acccgtcgtg gaatagagat agggggtatg cgcctcaaac
     3781 tctgcgccac aggtatccac tcttttaaat accgtgccat agccagatcc cctgacctgt
     3841 cgccattgac gaatggtatc ttcctctctc ccaatgagtt gaccgagccg ttgatctgaa
     3901 aatccaagac ttttcgcttg ttcaaggaga gtcaccaggg aagcagggca tcctttgcgg
     3961 gtgtcgtgtg aacgatcgtt cccaacttcc gccttgcttc tgcagaccca ttcagcctca
     4021 aattctacga gttcttcaat ttcatgtaaa aaccaggggt caacgcgcga cagatggtac
     4081 agttcctcca ccgacattcc gattcggaac gcatcagcca gaaaccaaag gcgatcagcg
     4141 gtcgggattc gaacctgaga ttgaagacgc tcatacaacg gatgagccga gccggcagag
     4201 tcctttttcc cattaagccc atgtaacgac atcaacccat accggtctgt ttcaagggaa
     4261 cgaatagcct tctgcaaagc ttctttgaag gtggatccga gtgccatggc ttctcccacg
     4321 gatttcatat gggttgtcag cacccgatca acaccatcaa atttctcaaa tgtaaaccgt
     4381 ggaattttca cgaccacata gtcgatggtc ggttcaaaac aggctttagt cacttgcgta
     4441 atatcgttgg ggatctcatc aagggtatat ccgacggcca acttcgcagc gatcttggct
     4501 atcggaaacc cggttgcctt tgaagccaat gcggaactcc tcgatacgcg tggattcatc
     4561 tcaataacca tcaaggcacc gttgtccgga ttcaaagaaa attgaatatt ggatccgccg
     4621 gtatccacac cgatttctcg aatgatgcgg atggccgcgt ctcgcatatg ctgatactct
     4681 ttatcagaca aggtcatggc aggggccacg gtgatactgt ctccggtatg cacacccatt
     4741 ggatcgaaat tctcgatggg gcagacaatg accacattat ctttggagtc ccgcatcact
     4801 tctaattcat attctttcca tcccagaagc gattgttcga gcaaagcctg tccaacagga
     4861 ctggtcacaa gagcccaatc aatatactta tcaaactctt cacgattata ggcgatattg
     4921 ccccctgctc ctcccagagt gaatgatgga cggacgatag cgggaaatcc aattgtttcc
     4981 agaagccgta cggcttcttc tcttgagcga aatgatccgc ttgcagccac gtttaatcca
     5041 atacgtgtca tcgcctgttt aaacaagtcc cgatcttccg ctttttggat cgattcatag
     5101 gaggctccga taagttgcac tccgtgcttt tccagaatgc cccgtttcac taattccact
     5161 gtgatattca gggcagtctg tccgcccatg gtgggaagca gggcatcagg gcgctctcgc
     5221 tctagaatgg cttccacgac ttcgaccgta atcggctcca cataggtacg gtccgcgagg
     5281 tccgggtcgg tcataatagt ggcgggattg ctgtttacga ggatgacttc aaagccctcc
     5341 tccttcaggg ccctgcaggc ttgtgttcca gaatagtcaa actcacagcc ttgcccaata
     5401 acaatcggtc cagagccgat taataaaatt cgatgaatgt cgtttcttcg tggcatgatg
     5461 ggctctcagg attgtaaaac tgatccagat atcggttttt tagcgggaga ttcaggtgat
     5521 tcgggaggtg atgtctcagg atgataaaat ttcaatgctt ggggtatcca ggcttcaatt
     5581 tgattaattc gggttacatc cgaaggatga gtggacagaa attctggaat cgcattttga
     5641 ctgcggaaac aaaattttcc tatcattttt ctcggacacc cactcatacg ttcccaaaac
     5701 ggcaccgctt ccctcggatc gtagccggcc ttggccatca attgaagccc gataaaatcg
     5761 gcttcggttt cctggtcacg ggaattggga agggccacgc ctactccata ggcgctcatg
     5821 gccccaatag ccaagccagg gtccacgccg ccggcggcgg ctccagccaa agcgccaagt
     5881 tgaccgattt gttccaaaat tccacgactg tatcgctcgg ctccatgccg ttgcaacgca
     5941 tgggcaactt catgtgccat gaccgttgcc agcccggctt ctgtttttgt gtattttaaa
     6001 atgcccgtaa acaccgccac tttgccacca ggcaatgcaa aggcattcac ttgatcgtca
     6061 tcctgaatga cggcgaactc ccagacatat tccggttttt gggctgcctt ggcaatgcgt
     6121 tgtcccacac gattcaccat taacgctatt tctggatccc tgcttaatgg tgccgacttt
     6181 aaaacctcgc gaaatgccgc aagtcccagg gcaatttcct tttcctccga aatataaatt
     6241 aattgatctc tagccgtccc cggagctttt tgacatgctg aacctagcaa caggagcgca
     6301 cacaccgcac tggccaagag ccattggaat gtcattccag aacccgatgg atcgttgatc
     6361 atgggaacaa catttcttac atcaggacca caccgatttt acggcatcca catgtacagc
     6421 atttgtggtg tccctccggt caatacgtta aagagagaat gcgcatcgac ctggggaaac
     6481 ccgataggcc caatttgagg aggatcgaac cgtgaataga tattctgatg agttcccctt
     6541 cccctcgtgt acgtgacgac tttggcctgt tccaaatttg cttctttttt ggccaaatca
     6601 atcgcctcat ccaaatatcc aatgctatcc acaagaccct tggatttggc aatatcggcc
     6661 gtaaaaatcc gtccgtcagc tagcgtgcga atctgctcca tgcttaatcc aggtcttccc
     6721 tctttcacca ccgaaaggaa ttgctcaaac aaatgatcaa tggtcccttg gaaaatagcc
     6781 cgctcttcat cattcattgg ccggaatgga gaacccatag cttttttagg acccgacaca
     6841 atcgccgccg gttggacccc gattttttcc aacagcccct ctgcattcat ggtcaccata
     6901 atgaccccga tacttccggt aatggtcgaa ggatgtgcaa gaatatggtc ggcggacatc
     6961 gcaatgtaat atccgccgga agtgcccaaa tccataatgg ccgcaatgac ggggacaacg
     7021 cgttttttct taaagacttt cagctcatga tataccagat ctgaggccgt gaccgatccg
     7081 cctggagtat ttattcgaag gatgatagct ttgacatgtt tatcctcttc ggctttggtc
     7141 aattcctctt tgatccgtgc cggaagactg ggttcctcca agatacccga tgctttgcct
     7201 gtcgtcagca tgccggagat atcaatcaat aataccttat cctttccctc gccagatatg
     7261 atggcttcct ggagttttcc tcctccggga aacagatcaa catggataca cccgctgacc
     7321 aaaaaaagaa aaagaattaa ataacaacga gtcatcgcca ttatcctggg cctttcatca
     7381 tttccagaaa ttgtcggaac acatatgaag agtcatgggg gcccggggag gcttccgggt
     7441 gatactgaat ggaaagaatt gggctctcca agcctgccat cccttcacaa cacccatcat
     7501 ttacactccg atgcgtgatt tccattcgtc caacacagga gtcaaactgt tgagacgtca
     7561 acgttgtaca gacgtcttcc ttgtgaggaa ggccaacagc aaaattatgg ttttgcgagg
     7621 taatttcaat ctttcgggta cggagatcca tgactggatg gttggctcca tgatgcccaa
     7681 aggttagttt atgggtgtgc aatcccaggg ataatcccaa tacctgatgt cccaaacaaa
     7741 tccccaacag tggtttccaa cccaatagct gcttgacggt ttctacggca tagggcaccc
     7801 cttcggggtc tcctgggcca ttggataaca caatcccatc tggattaagt cgacggactt
     7861 cttccgcggt cgtcgaagct ggcacaactc gaacctgaca tcccagatcc acgagacttc
     7921 gtaaaatatt ttgtttcaca ccaaaatcca tgaccaccag tttcaaaggg ggttgagcgg
     7981 tgttttgagt gaaaatggat gggtcaccac tagccgggtc cataacaatg gatctctcag
     8041 tccattcata agcgtccgga caggtgacct ctctcaccaa atcacgtcca accagagaag
     8101 gcagggactt cgccttttcc cgcaaaccgt tgtcattgaa atctacatgg gaaatgattc
     8161 ccatttgtga gccgtgatca cggacatgac aggtcagtgc tcgcgtgtct atcccttgaa
     8221 tggccacaat acgatgctca attaaatagt cgtcaaaaga ttgccggctc cgccaattac
     8281 tccagtactt ggccgcttca cgaacaataa atccttccgc ccagacccga tgcgattcat
     8341 tgtcttccgg ggtcacccca taattcccta tttgggtgga ggtcatcagc acgatctgtc
     8401 ctttatagga agggtcggtc aaaatttctt gataccccgt catggccgta ttaaatacca
     8461 cttccccttc ggtttcgcct tcgaaaccga gtgcacgtcc ctgaaagatg gttccatcgg
     8521 ctaacgccag aactgcgggc ttcatggtct ttcctttttc atcttcttat ttccgttgta
     8581 cgatcaaagg taattatgag cgaggagtta tggtgtcgtg aaagagagct ttccgtacct
     8641 ttacaataaa cagtgaagcc gcgagaccaa agttcaccag gtggcaggct cgtacgacca
     8701 tatgaagacg aaaaaatttt gcataggcga tgtctttgtc ttcttttgag accgcatcaa
     8761 aggcctcttc ctgtaaagta atggcttggg gacccagaac caccaagatg ctgaacgtca
     8821 tgagggccat tccggccaga agacaccact ccacggaaga cacggcaaac atctcggagg
     8881 ggttatggcc aaaagctcgg aagcgaatca cggccaccac actcaacaaa atcaaaatcc
     8941 aggcattcat caagccaaat ccatcaaaga ctctccgtaa aaagcggcca gcgggttcca
     9001 tcccaaagga attaaaaacg gccggaatca ccaacgcaat aatgacgact aacccgccca
     9061 cccataagat taaggcacac agttcaaaaa gaaaacccac gaccccccag ttaatccgct
     9121 tcatactttt gcattaacgg gcatcataga ccatcctgcc atcaaccaat gtcatcttca
     9181 cctttccctt cactgtccaa cccccaaatg gcgtattctg actttttgag aaaaatttcg
     9241 tgggatccac cacccactct tcatgaggat caaacaccac gatatcggca tgtgtccctg
     9301 gctggaggct tccaacaggc agatgaaaaa gctgagcagg agatctggtc aatttatcaa
     9361 gcgcctgttc aagggacaag accccctcat gcaccagacg gagggttaag ggaagagcgg
     9421 tctcaaaccc tacaatccca aacggagccg tatcaaattc caattgtttt tcttggagag
     9481 catggggagc atggtcggtg gcaatggcat caatggtacc atcaaccagc ccctgcttaa
     9541 gggcttgaac gtcttcatcc gtccgcagtg gtggattcat tttggcattg gaattgtaac
     9601 agcggacggc ttcttcagta atggagaagt gatgaggaca gacttctgcg ctcaccggca
     9661 acccttgccc ttttgcatga cggaccagtt ccaccgaacg ggcggtgctc aaatgggcca
     9721 gatgcacatg aactccggta agatccgcca gagccaggtt tcgagccacc atcacctcct
     9781 ctgaggcatc gggaatccct ggcattccga gttcagtcga taccatgccc tcgttcatgc
     9841 atccgccatc ggttaaatgc aggtcttcgc aatggtccac aacaggaatt ccaaaagcct
     9901 tggcatactc cagtgcccgt cgcatgacca aactattcat cacgggacgc ccatcatccg
     9961 aaatggccac acatccggcg tccaccagct caccaatatc cgcaagttct tctccttcgg
    10021 attttttggt gatggccccg atcgggaaca ccctggcctt gccggcctct tgtccttggg
    10081 ccaacataaa tttggtgatg gcagcgttat cattgacggg ctgggtattc ggcatacaac
    10141 aaatggtcgt aaatcctcct gccacggccg aggccgcgcc ggtcgcgatg gtttctttgt
    10201 attcaaaccc gggttcccgt aagtggcaat gcagatcgac aaggccaggt gccacaatcc
    10261 agccattggc ctcaagaatt gtaactgatg aatcctgaca aagggggttt gtcaggggga
    10321 aaccgatctc aagaatattt ccatcttgaa taagcacatc ggcccgtcca ttcacgtggc
    10381 cggggtcgat tacgattcca ccttgtatac agagcagatg agaagaagta ggagacagtc
    10441 ctgtcttttt ggtcatgttg tccgattact cgttaagggt tgaacgttca ctagatcctg
    10501 ataataaata cataagggcc attcgcaccg agaccccatt ggtcacttga tccagaatta
    10561 ccgcgacatt gctatccgcg accgatggtg aaatttccac gccccggttc agaggaccgg
    10621 gatgcatcac aatggcatcc ggtttggcga gtttgagtct ttccggagtc aaacaaaaca
    10681 gtttggcata ttctcgaaga ctgggaagta acgcccgccc taagcgttca cgttgcaatc
    10741 gcagcatgat gatcacatcc gttccaatca atgcctcatc aagatcgtag aatacctcca
    10801 cgccgaaatg ttcgagaccg aaggggatca tggttggtgg agctacgact ctcacctctg
    10861 ccccaagttt ggtcagagca agaatattcg agcgagccac gcggctatgt gcaatatcgc
    10921 caacaatcac aaccactaag cccgagacgc gtcctttttt ctcttttatg gtaaagacgt
    10981 ccaagagggc ctgagtggga tgttcatgcc acccatctcc cgcattgatc accgatgatc
    11041 tgaccgaacg agcaaggttt tctgcggcac caggagatgg atgacgcagc acaataatat
    11101 ccgcctgcat ggcttcaata tttctagcgg tatcgactaa ggtttctcct ttcaccatac
    11161 tgctggtcga aggcgaaaaa ttaatgacat ccgcactcaa tcgtttagcg gccaattcaa
    11221 aagatgttcg ggtacgagta ctgggctcaa aaaataaatt caccaccgtt ttcccgcgca
    11281 gagccggaac ttttttaatg tcccttcctc cgacctcttt cagggattcg gctgtcgtta
    11341 gaatcaattg aatttggtca gctgataagg ccgcaatagt caataaatct ttgcgttcta
    11401 gacccataat gattttatgg tgttaaaatg gagacccgat cgtcctgccc taattcctca
    11461 aaaaagacct ggacgttttc ttcccgggca gtcgggatgc ttttcccgac ataattagca
    11521 cgaatgggaa gctgccgatg tcctcgatcg acaagaacag ccagttgaat ttcggcaggg
    11581 cgccctaaat ccattaaccc atccaaggca gcacgaatgg ttcgtccagt aaacagtacg
    11641 tcatcgacca gcacgatttt caaatcggat atatgaaagg gaatggtcgt tttccgaatt
    11701 tccggctgat cttttctgat ggaga
//
LOCUS       DATIAL010000514        15762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1273918, whole genome shotgun sequence.
ACCESSION   DATIAL010000514 DATIAL010000000
VERSION     DATIAL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273918"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VLA60_15790"
     CDS             <1..228
                     /locus_tag="VLA60_15790"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HSF10874.1"
                     /translation="LAPSSSLRKWFGHDPDKWEAFKERYRKELSSAEPREALKEFAEL
                     SRQKTITLLYGAADEEHNQAVVLKEVLEELA"
     gene            300..1073
                     /locus_tag="VLA60_15795"
     CDS             300..1073
                     /locus_tag="VLA60_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF10875.1"
                     /translation="MNDVASEDQGSGLNAIKASGYRVRLIARREVAQRTMALYFARPA
                     GFSFKAGQFIDLTLLDPPETDSEGNTRAFTLAGAPSEEHLMVVTRLRDTAFKRVLNEM
                     PFETVVNIEGPFGNLTLPETVVSPLVFLAGGIGITPFRSMLVQAATQHLPHRLVLFYS
                     NRHPEDAPFLHELQDLQRKISSYTFVATMTQMEESDDSWTGETGYLDHAMLTRHLQGI
                     ESPLYYIVGPPAMVKALRAMLKNAGVRQVDIRTEEFAGY"
     gene            complement(2235..3179)
                     /locus_tag="VLA60_15800"
     CDS             complement(2235..3179)
                     /locus_tag="VLA60_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HSF10876.1"
                     /translation="MKKLGRSSQLWSVILAGGDGVRMQPMIQQWLGKPKPKQYCAFVG
                     TRSMFQHTVDRALRLTEPERTVTVIARSHEPEAWSQLDGRVCGKVLVQPKNCGTGPGI
                     FLPLTYIRAKEKNGTVVIYPSDHFIYPEDRFLEIVELAGWIVERMPHRPILLGAPADR
                     LEVEYGWILPDEVHSVVDGYRLSTVDRFLEKPNVIQARQALAANGMWNMLVLVTKIET
                     IWSLGWKYFPEMMVLFERLEKVIGTSEECTVLNAIYGVMPNKDFSFDLLQHAPRRMMA
                     LELNRVLWSDWGQPDRIEKTLAKLDKKPAFSMDSLASV"
     gene            complement(3662..4051)
                     /locus_tag="VLA60_15805"
     CDS             complement(3662..4051)
                     /locus_tag="VLA60_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10877.1"
                     /translation="MERITGRDSTRYRRNTIMTMKNRIYVRAQIFVHESQNGEYCIPY
                     KTLIGYGIGNRVIVDGAGEHLYCGQVFESMETAANEAKRNALKTMKKKFPHVEEQRII
                     WDVVPERALGLDYGSPRRVFLEEFATT"
     gene            complement(4764..6206)
                     /locus_tag="VLA60_15810"
     CDS             complement(4764..6206)
                     /locus_tag="VLA60_15810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10878.1"
                     /translation="MKSFRNQIRHSALSLVIGLLAGFTVLVYWSFEAALNRYVDSRLV
                     TLATTLGKLINDQPHLLQKSKQEINFDGTSDEEQRSLREASHSIQVLSPNGEIIWKGA
                     AAIVRSPITDILLSQVQRGQIVHDTIYLVNATPVRRVSVPVPRQSEPQYIIQAETSLR
                     FSQEALSSLFILLAGFSSITVAIAWLGSDWVARKALSSLKALSAAAENVTGPPFQKPL
                     LLDPPYQEFSRLTNAFNAMLDRLQKIFEGQRHFVDHAAHEMQTPLTVLQANIEVTLQK
                     ARTVIEYREALLTNLEQVERLGTLTRSLLILARFSDDRYAVQLVPLELEPLVQDLISE
                     LMLLAKDQQILLTLESQPVPLVMGDRERVKQLLINLLDNAMRYTSPGGTVVVRMGLKE
                     DYVAIAVEDTGVGIEPEHLPRLFDRFYRTDTSRVKNSGGTGLGLPIVKEIAEAHRGTV
                     TVQSEIGNGSVFTLLLPPCKDLPQSSGVTI"
     gene            complement(6203..6886)
                     /locus_tag="VLA60_15815"
     CDS             complement(6203..6886)
                     /locus_tag="VLA60_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HSF10879.1"
                     /translation="MRILLVEDDSDLAQFIRKGLQEEGYAIDQATNGEDGFLFASTYS
                     YDLLILDVMLPQLDGMELCRRLRTKGHTTPMLFLSARDSIQDKVTGFDNGADDYLTKP
                     FAFAELLARIRALIRRVGPHILRRLKAADLEIDPSSRQVWRAGNEITLTNKEYSLLEY
                     LIRNKNRVLTRTAIIEHVWDINYNPMTNIVDAHVRALRSKMDRDFSPPLIKSVRGAGY
                     MLEAPEEAI"
     gene            7288..7710
                     /gene="rnk"
                     /locus_tag="VLA60_15820"
     CDS             7288..7710
                     /gene="rnk"
                     /locus_tag="VLA60_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HSF10880.1"
                     /translation="MEYRDIYITEFDLNRLSELLEVGLSFRGSKSESGHLDGLKEELD
                     RAHIVLPKDIPPDTVTMNSRVRLSDVSKAGELVYTLVFPRDADAATGRISVLAPVGTA
                     ILGCRVGDIIEWQVPAGKRKLKIEEILYQPEAAGDYHL"
     gene            8052..8312
                     /locus_tag="VLA60_15825"
     CDS             8052..8312
                     /locus_tag="VLA60_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HSF10881.1"
                     /translation="MVLTHQKGLWVDERPLVSHNTALADKPLSGLIDFERQHILLVLA
                     SSNWEIEEVSGAAERLELAPSTLRNRIKMLGLQWSSSSTSQK"
     gene            8639..9421
                     /locus_tag="VLA60_15830"
     CDS             8639..9421
                     /locus_tag="VLA60_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10882.1"
                     /translation="MFSLMILPIGVLISAPSPSMVFAAADLNERYHELLSGDRILLAT
                     VEEIRGGEAKADTGELQPRYLPINVRKDKGLPALKKGDRVEITVNDQNLIVDIHLVGE
                     ESHHRILVGELAQPLITGQEKAVIRTEDGKAESHLVRPLARSKVASVPVGAPAVFLID
                     EMQGIADVTYGSVEAVKMAQHLWKKKSPPKASFSKVTGVILKPLDNNTITIQTEEAQV
                     HSYEVRPLVLPKVATLPKGELIVLFVDDENKVTDVSTSHSKK"
     gene            9485..9919
                     /locus_tag="VLA60_15835"
     CDS             9485..9919
                     /locus_tag="VLA60_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10883.1"
                     /translation="MLTQGIPVGGLNTIGQIVATNNLQFYAHQNGLAIAVALLSTHTA
                     GSPVVDGQGRYVGFINEFDVMRALAAGKDLGKLAAEDIMRKDQLAVTPSTKIADASAK
                     MEEHHVFNLPVIDQNGVVTYTVSRHDLLRAWIGLGVGMGIDP"
     gene            10394..10696
                     /locus_tag="VLA60_15840"
     CDS             10394..10696
                     /locus_tag="VLA60_15840"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HSF10884.1"
                     /translation="MVVSELSGARVFALHVHDDSMEPLFPEGEIIFVNPDFELQPGHY
                     GIFLDQTGGADPGCLRQFKKIQERYVVHSLNIQYKDTPLTSHQKVIGRVIRLRMKL"
     gene            11140..11391
                     /locus_tag="VLA60_15845"
     CDS             11140..11391
                     /locus_tag="VLA60_15845"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HSF10885.1"
                     /translation="MLELYQFEECPYSTKVRKKLSEWEIDCILRNVSQDKSKRSLLWK
                     ISGQDEVPTLIDSDRDAIIAGDEKRIIQHLETYYARRST"
     gene            11529..12131
                     /locus_tag="VLA60_15850"
     CDS             11529..12131
                     /locus_tag="VLA60_15850"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HSF10886.1"
                     /translation="MKGTQQYTAKEFNLSGLKGISDKTLEMHFKLYEGYVKATNTLHK
                     LIANILTDGKVDQEEVPAYSELTRRLGFEYNGMVLHELYFENMTSQASSGFKGQDSLG
                     KAITESFGSFENWKTDFSSIGKMRGVGWAICYQNPHNGRLSNHWVSLHECGNVAGFRP
                     LLVMDVWEHAYLLDFQPSERGAYIESFLLNVNWDVVAKRQ"
     gene            12157..12663
                     /locus_tag="VLA60_15855"
     CDS             12157..12663
                     /locus_tag="VLA60_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HSF10887.1"
                     /translation="MGIVQHYLAEDHARLDNLLQRAIDKQEKIDHQAYKEFRRGLLRH
                     IAMEEKVLFPTIQRLRSGTPLPLTDKLRLDHGALGTLIMPTPTRAIIELIRTILVSHN
                     GLEEGPEGVYAQCEQVAGVGIEDLFRRLQAVAPVSVAAYSDSPTVFATIRRVVMRAGY
                     PAESMEFH"
     gene            complement(12870..14114)
                     /locus_tag="VLA60_15860"
     CDS             complement(12870..14114)
                     /locus_tag="VLA60_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HSF10888.1"
                     /translation="MQKRSKTEEEDRANKDSLMDIIRAGAHQLIAQALKAEVTELLAT
                     YADQRDEQGHARVVRSGHHPERAIQTGMGPVTVRVPKVRSRHGDPVRFHSALVPPYVR
                     KTASLEAAIPWLYLKGISTGEMQMALEALVGPEAKGLSASTVARLKQGWREEYETWRH
                     RRLDADQWVYLWVDGIYSGLRAETQRLCALVVIGMNAHGEKHLLAIEDGIRESTQSWR
                     EVLLTLKARGLTAPALAIGDGAMGFWGALEEIYPSTQQQRCWCHKTRNVLNALPKSVQ
                     PKAKQALHEIWQAPTKATAEDAFDVFINTYDVKYPKATACLQKDQDALLTFYAFPAAH
                     WPSLRTTNPIESTFGTIRHRTARTKGCLTRDGRLHMMFKSGQCAEKTWRRLRGFNELL
                     KVVAGIQFIDGLEQAATDSVAA"
     gene            14296..15389
                     /locus_tag="VLA60_15865"
                     /pseudo
     CDS             14296..15389
                     /locus_tag="VLA60_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612008.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(15554..>15762)
                     /locus_tag="VLA60_15870"
     CDS             complement(15554..>15762)
                     /locus_tag="VLA60_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10889.1"
                     /translation="DTANVLTAAGISTIAPLDSKEQIMRGLVKFLSLIREGHAPIPNK
                     AEVIKHSRQARTAELAKLLDSITE"
BASE COUNT         3943 a         3923 c         3895 g         4001 t
ORIGIN      
        1 ttagcgccaa gcagttctct ccgcaaatgg tttggacatg atccggacaa atgggaagcc
       61 ttcaaggagc ggtatcggaa agagctttcc tctgccgaac cgagggaagc gcttaaagag
      121 tttgcagagc tttcccgtca aaaaacgatc accctcctgt atggtgccgc tgacgaggaa
      181 cataaccagg cggtggtgtt gaaagaagtc ctggaagagc tggcttaggt gcgccggccc
      241 aatgaatggg gggaccgtcg tggcgctgcc ttggtcagaa gaagaaagga aattacgtaa
      301 tgaacgatgt cgcgagtgag gatcaaggat cgggtctgaa cgccatcaaa gcttcagggt
      361 atcgggtcag attgatagca cgccgtgagg tggctcaaag gacgatggcc ttgtattttg
      421 cacgcccagc cggcttttct tttaaagccg gccagttcat tgatctcaca cttcttgacc
      481 cacctgaaac ggactcagaa ggcaacaccc gagcctttac cctcgccggc gcgccgtcgg
      541 aggagcacct catggtcgtg acccgattac gggatacggc ctttaagcgc gtgttaaatg
      601 agatgccttt tgaaaccgtg gtcaacattg aaggcccctt tggaaacttg acgttgcctg
      661 aaactgtagt gagcccgctg gtttttcttg ccggcggaat tgggatcacc cctttccgga
      721 gcatgttggt gcaagccgct acgcaacacc ttccccatcg cctcgtgctt ttttactcca
      781 accgtcatcc ggaagatgcg ccgtttcttc atgaactcca ggatcttcag cggaagattt
      841 ccagctacac cttcgtggcc acgatgaccc aaatggaaga gtccgacgat tcctggaccg
      901 gggagaccgg gtatctggac cacgccatgc tgaccagaca tttacaaggg attgaatctc
      961 ccctctacta tattgttggt ccaccggcca tggtgaaagc cttgcgagcg atgctcaaga
     1021 acgctggtgt tcgccaagta gacatacgta cggaagagtt tgctggatac tgagacagtt
     1081 ggagggtgca ctccggcaga tgcgaaacct cctgcccgca aaactggctt cgagatcacg
     1141 tggttaatat tcttgtggat cgtccctttg aaagcctgtc ctctgacagg aaaagaagtg
     1201 tgaaaaaccg gctcgagtcg attacgctac tcgaatgttg tgccatcgag atgctctttg
     1261 ttcctcagac ccttctttga atttctcctg agccccgtca ttcgacgatc gctggcgctc
     1321 aatcgataag tgtgacgggt tcgatgaagt tgaccctttt cttttttttc cgaggctcag
     1381 aatttcgtta aggtattggt cccgcataca gcgaataact cgcaaccgtt tgggtcggtc
     1441 tagagaccac caagtcagag agttcaagtc attcacggtt ggaggcgaca ttttctccgt
     1501 aggaccctct ccaaaattat aacctcttgc atcttggctg gcttcgtaaa aattgtgata
     1561 taagccttcg tatggtccgt agggcttcca atgcgttttg ttcattgttc tctgtctcct
     1621 ttcttcagga aagggcgtaa cccgcgaatc cacctggttc tggatattga cccacctatt
     1681 tacccgtaag accatagaat tttccttttc cttatcatcc ccttctggtc tttccgtgga
     1741 aactgggacc tcgttccact tggggtgtct gtcatgcccg tttgtagggc atgacagact
     1801 cgcggtgatg catctggtta cgaaaattat cccttaatat ttaggcacct tttttatacc
     1861 gattttttgc gagtgacccg tcttcaactg agataggtcg ttgttcacca ggttcaccgg
     1921 gatcttctct gtaagatatc caaacggctg ctgcccacaa tatgaacatc acactcagca
     1981 tggttatcgt ggcttccatc aacatgttgg gtcctccttt gaatgatggt gagtaatagt
     2041 gccggttttt ttaatcggat gaattcttca aatgaaaaac atgagagatt tagctccatc
     2101 catctcacgc cgacaacagg actatgcctg tctgtttttt tctagaaggt tgctttaaac
     2161 ttgctattcc ccgtagcttg ctgcggggat cgttattccg gctttcgaga tcctcccttg
     2221 attgctgtag taggttaaac cgacgccagg gagtccattg aaaaggccgg cttcttatcg
     2281 agctttgcca aggtcttctc aattcgatct ggttgacccc aatcactcca aagaaccctg
     2341 tttaactcca atgccatcat tcttcggggg gcgtgttgaa gtaaatcgaa tgaaaaatcc
     2401 ttgtttggca taactccata tatcgcattc aaaaccgtgc attcttctga agttccgatg
     2461 accttttcta gtcgctcgaa taacaccatc atctctggaa aatatttcca tcccagactc
     2521 cagattgttt cgattttcgt caccaacacc agcatgttcc acatcccatt ggccgccaaa
     2581 gcttggcgtg cctgaatcac attcggcttc tcaagaaatc tatcaacggt agataggcga
     2641 tagccgtcca ccaccgaatg aacctcgtca ggtaggatcc agccatattc cacctccaat
     2701 cgatctgcag gagctcccaa cagaattggg cgatgtggca ttctttcaac tatccatcca
     2761 gccagctcaa ctatctccaa aaaacgatct tccggataga taaagtgatc agatgggtag
     2821 atcaccaccg ttccgttttt ctctttcgcc cggatataag tcagtggtaa aaaaatccct
     2881 ggtcctgtgc cacaattttt gggctgcacc aagacttttc cgcaaactct cccatccaac
     2941 tgcgaccagg cttccggttc atggcttctc gcaatcacgg taacggttcg ctccggttct
     3001 gtgagtcgga gcgcccgatc gactgtatgc tgaaacatgg atctggtccc gacaaaggcg
     3061 cagtattgtt ttggcttggg cttacctaac cactgctgaa tcatgggttg catccgcacg
     3121 ccgtcacctc ccgccaaaat gacagaccaa agttgagatg atcgtcccag ttttttcatc
     3181 accccctcct tttcatggaa gttgtcgcaa agtagtttta ttcttaatgt ttggcactat
     3241 acctacacaa catgaatgga ttatgaatga accatgaaga attcttcatg aatcaatccg
     3301 accgtttatg ggaatgctaa atgtggagaa aatcactaag gaggcggaaa tattttggag
     3361 cgaaactccc tgcccattgg gcagggagct tcacttcccg gacaggcctt tcgaggattc
     3421 gagtgcaact aaaggaatta aatgagttgc aataacccat cccgttctga attttccagc
     3481 aggcgatttg aaagtgtggt aatcctgtgg gtttctttct ggtgcaatcc ttacgcgttt
     3541 tgcgtatttt agccttcttt cttaggaaaa ttttgagaat cattagccat ttgccggatt
     3601 cccaaacccc gtactatttc gtgctgtcgt agatcccact ccagcgggca tcattgagcc
     3661 ttcaagttgt agcgaactcc tcaaggaaca cccttcttgg tgatccatag tcgaggccta
     3721 aggcccgctc aggcaccaca tcccatataa ttctctgttc ctccacatga ggaaattttt
     3781 tcttcatggt cttcaaagcg tttcgcttcg cttcattcgc agctgtttcc atactttcaa
     3841 atacctggcc gcaataaagg tgctccccag caccgtcaac aattacccgg ttaccaattc
     3901 cgtaacctat aagggtttta tagggtatac agtattctcc attctgactt tcgtgaacaa
     3961 agatctgagc cctgacgtaa attctgtttt tcattgtcat aattgtattc cttctgtatc
     4021 tcgttgaatc cctcccagta atcctctcca tccgttttcc tgactagcca cgagagaatt
     4081 cagtcattat aggaatgccg gtcctcaggc catcgacatc ggtttgtctc ttgaccgatg
     4141 ttgattactt tcgatccaca ttaccaatct cctgttggaa aatgactagt aagggcggta
     4201 ttggacaaag aaggccgtgc acaggcccag gcatgagtat gatttccaca ctgacctgag
     4261 tcataccgcc atgtagttgt aaaggtattt ggggcatcgt caatgcctcc tgtctgaaat
     4321 tggtccttcg tgccgatcta ctctgttccc ttttgaatga tttccctcaa gaaggtgaca
     4381 gtaccgtatc tgcatgaaca agccatgaac agaacatgaa gaaattttca tatagggagt
     4441 attgaataat aaaaaattcc aagggcatat tgacccacag gcacattgca tatctaaggc
     4501 ctcggggcgg ttcaaaaaaa ttcgtccagc aaggccgtag tcatttttgc gcgcagagcg
     4561 tacttccagt acgtgagcac ggagaaatga cgagaacgct gctggcggct tttttcaaca
     4621 gccccatata ggattgtgat ccattaaccc ttccacgagg ctattggacc cgtggtacag
     4681 ggaggcattc gaaggtcaag aacaaccgtt gggggttatg cagaggtgtt gacggctgga
     4741 agataggagg aaaaaacaaa ttcttagata gtaacaccag aagattgagg aagatctttg
     4801 catgggggaa gaagcaaggt gaacacggaa ccgtttccga tttcgctttg gacggtgacg
     4861 gtacccctgt gagcctcggc aatctctttg acaataggca atcccagacc ggtccccccc
     4921 gagttcttta ctcgtgaagt gtcagtccgg taaaaccgat cgaaaaggcg gggaagatgt
     4981 tctggttcaa ttcccacacc ggtgtcctcc acggcaatgg ccacataatc ttcctttaat
     5041 cccatgcgga caacgacggt tcccccagga gatgtataac gcatggcatt atcgagtaaa
     5101 ttgatgagta actgttttac ccgttcccga tcccccatca cgagtggtac cggttgagat
     5161 tcaagggtga gcagaatctg ttgatcttta gcaagtaaca tcagttcact gatcagatct
     5221 tgaacgaggg gctcaagttc aagtggcacc agttgaacgg cataacggtc gtctgaaaat
     5281 cttgccagga tcaacagtga acgggtcagc gttcctaatc gctcaacctg ctccaaattt
     5341 gtgagcagcg cttctcgata ctcaataacc gttcgagcct tttggagggt cacttcgata
     5401 ttggcctgca gaacggttaa cggagtctgc atttcatgag cggcgtgatc gacaaagtgt
     5461 ctttgtcctt caaaaatttt ttgaagccgg tctaacatgg cattgaacgc gttggtcaac
     5521 cggctgaatt cctggtaagg gggatcgagg agcaatggct tttggaacgg tggtccagtg
     5581 acgttctccg cagccgcact gagggccttg agggaggaca gggcttttct ggctacccaa
     5641 tcgctcccta gccacgctat ggcaaccgtg atacttgaaa atcccgcaag caagatgaaa
     5701 agactgctca gggcttcttg tgaaaagcga agagaggttt cagcctggat gatatattga
     5761 ggctcacttt gccttggcac gggaacagaa actcgtcgga ctggagtcgc gttgaccagg
     5821 taaatggtat catgcactat ctgacctctt tggacttgcg agagcagaat gtcagtgatc
     5881 ggagatcgaa cgatcgcggc tgcgcctttc caaataattt ccccgttagg ggacagcact
     5941 tggatggaat gcgatgcctc ccgtagcgaa cgttgttcct catcagaggt cccgtcgaaa
     6001 tttatttcct gtttcgattt ctgaagaaga tgaggttggt cattgatgag tttcccaagg
     6061 gtggttgcga gagtcaccaa tcggctatcc acataccgat tgagcgccgc ctcaaaactc
     6121 caatacacca gcacggtaaa ccccgcgagc aggccaatga cgagcgagag agccgaatgt
     6181 cggatttggt ttcgaaatga tttcatatgg cttcctccgg tgcctctagc atatatcctg
     6241 ccccgcgaac cgatttaatg agagggggag agaaatccct gtccattttg gatcgtaatg
     6301 cccgaacgtg tgcgtcgaca atattcgtca tgggattgta gttgatgtcc cagacatgct
     6361 cgataatggc ggtccgtgtt aatacccgat tcttgttccg tatcaaatat tccaggagcg
     6421 aatattcttt attcgttaat gtgatctcgt tccctgcccg ccagacttgt cgggatgaag
     6481 ggtcaatctc cagatcggca gcctttaacc gtctgagtat gtggggaccc acgcgtcgaa
     6541 ttaaggcgcg tatccgagca agcaattctg caaacgcaaa cggttttgtc agataatcat
     6601 cggctccgtt gtcgaacccc gttaccttgt cctgaataga atcgcgagcc gagagaaaga
     6661 gcatcggggt cgtgtgtccc ttggtccgaa gccggcgaca caattccatg ccgtccaatt
     6721 gcgggagcat gacatccaga atgagaagat cgtatgaata ggtgctggcg aaaagaaacc
     6781 cgtcctcacc atttgttgcc tggtcgattg cgtacccctc ctcctggaga ccctttcgaa
     6841 tgaattgggc caggtcgcta tcatcttcga caagaagtat tcgcatggta cgactccggt
     6901 tatgcctctt ttttctacaa ggagattctt aaaccttctt tatcctcgtt cctgagtaaa
     6961 tcatgcatgc ttctgtcatg gcagtgctgc aaatacataa gctggattgg aatagtgatt
     7021 cagacaagat acaggcctat aaaagcccag acaaccgcta ccttgaatca ccactccgtt
     7081 acgaggatag agacttttct tctcatgtat gccaccggtt ctcgttaatg aaacaggagc
     7141 actcgtctac agaaggctga tttgtaagaa aaggaaattt ctcaaattcc taaggcagtt
     7201 cgtttttcat gttagcttaa ccttttcaag tcctaggttt ttcttcaaat cttatgataa
     7261 tcttcgccaa taaggagaag tcacacgatg gagtatcgag acatttatat tacagaattc
     7321 gatctgaaca gacttagtga actcttggaa gttggtctct cgtttagagg cagcaagagt
     7381 gaaagtgggc atctggatgg cttaaaagaa gaattagacc gggcgcacat cgtcctccca
     7441 aaggacattc cgccggatac cgtcacaatg aactcacgtg tccggttatc agacgtgagc
     7501 aaagcaggtg agctggtata taccctggtt tttccgcgtg atgccgatgc ggcaacaggc
     7561 agaatttcgg ttttagcacc agtgggaacc gccattctcg gttgtcgggt tggagacatt
     7621 attgaatggc aggtgcctgc aggaaaacgg aagctgaaaa ttgaagagat tctctatcaa
     7681 cctgaagccg ctggagatta ccatctgtaa tccccctctg gctctatcgc gttattaaca
     7741 tacgcaccat gggaaagtta aaggattaag gacgattttc atcgaattca ataaataaat
     7801 tgccctaatg gtttgtctga aggcaacaat ttttctactc cagtgatgat tgattcgagc
     7861 catcaacact gaccttattt atgggttgcg gcctcctacc aattgcatga aagcgggaca
     7921 tgccatttag agactgtcga ccgcatgaga agacaggctc cacatcatgt atgggatcag
     7981 gacattcata tgagttaatt tcgatgagcc cacaaggcgg agttataata tttataaatg
     8041 gtaaaaatgc agtggttctc acacatcaaa aaggtttgtg ggtcgatgaa cggcccttgg
     8101 tttcccacaa cacggcgctt gcagacaagc ctctgtctgg attaatcgat tttgaacggc
     8161 aacatattct cctggtcctt gcctccagca attgggagat tgaagaagtg agcggagcag
     8221 cagagcgact cgaattggcc ccaagcacgc tccggaaccg aataaaaatg ttaggccttc
     8281 aatggtcaag cagttcaacc tcccaaaagt agtttttgag gaacttcctt cccttcatag
     8341 tcccccttaa atcctaaaat ctgtttgcca cgatccttcg tggctgtagc ccacgaccac
     8401 tcatcgcctt tcgtggctaa aaagtatcca ttcagttttt cctgcaaaaa taaaaataat
     8461 atgttgtttt tatttaattt ttgacgatta aaaaatcacg aaaccattgg tctagaagat
     8521 gcatgagtta gggcaataaa caattttaat caaataaagg agttccctaa tgacaagttc
     8581 aaggccacag agactaacga caactgaaca tagaacaata cgacccgtga tagtttccat
     8641 gttctcttta atgatattgc cgataggtgt tttgatttcc gccccttccc cttcaatggt
     8701 gtttgcagcc gctgacttaa acgaacgtta tcatgaactg ctctctggag accgcatcct
     8761 gcttgctacg gttgaggaaa tcagaggggg cgaagccaag gccgacacgg gggagctaca
     8821 accccgttat ctgcccataa acgttcgtaa ggacaagggt ttaccggcgc tcaaaaaagg
     8881 cgatcgtgtt gagattaccg tcaacgacca aaatctgatc gtcgatattc atctggtagg
     8941 ggaggaaagt caccatcgca ttctggtcgg ggaacttgcc cagccgctca tcaccggaca
     9001 ggaaaaggct gtgattcgaa cggaagatgg gaaggcagag tctcacctcg tgagaccact
     9061 tgcgagaagc aaggtggctt ccgttccagt aggtgccccc gcggtgtttc tcatcgacga
     9121 aatgcagggg attgccgatg tcacctatgg gagtgtggaa gccgtcaaaa tggctcaaca
     9181 tctgtggaaa aagaaatcgc cacccaaggc tagctttagt aaagtgaccg gcgtgatcct
     9241 aaaaccattg gacaataaca ccatcactat tcagactgag gaggctcaag ttcactccta
     9301 cgaggtccgt ccactcgttc tgcccaaggt cgctacgctg cccaaaggcg agctgatcgt
     9361 gctctttgtg gatgatgaaa ataaggtgac cgatgtgtcg acctcgcatt ctaaaaaatg
     9421 agggaggtca ggtttttctg actattaata gaaatgatta acaaaaaaga aaaaggagaa
     9481 aaccatgttg acccaaggca ttccggtagg aggcttaaac acaataggac agatcgttgc
     9541 gaccaacaat ttacagtttt atgctcatca gaacggcttg gccatagcgg tagcgctgct
     9601 gtcgacacat acggccggtt cgccggtggt ggatggtcaa ggacgctacg tcggctttat
     9661 taatgaattc gatgtcatgc gagctctggc ggcggggaaa gatctcggta agttggcagc
     9721 tgaagatatc atgcgtaagg atcagctggc cgtgacgccc tcaacaaaga tcgcagatgc
     9781 ttctgcgaag atggaagagc atcatgtctt caacttgccc gtgattgacc aaaacggcgt
     9841 cgtgacttac acggtctcac ggcatgatct cttgcgggcg tggatcggtc tgggtgtcgg
     9901 catgggaatc gacccctaag cctcggcctg tcgttttcgg tgatgggcag ggaccaaaac
     9961 tgccatcggc cacccaattc tcaagtggtc gatggcacaa gagaggagaa tatctagaat
    10021 caacagtctc ggaaaaaggt cctgcccatg aagagcctcg agaatctcat tcaataccaa
    10081 ttaagtgaag gcctgaaaga gcaccaatag gcagaagaca ttggcgtgcc caccttcaca
    10141 attcatggga ttcttaaggg cagagatccg aagagtccag gtgtctggag gagattgtct
    10201 cgctcttttc acatggatgt gaatctcttg cgtttcggta aacccggcct ctcgaaccca
    10261 caggagaaag acgatgaccc aactgggcat cctcagccca atcaaaaggt gccgatcctt
    10321 tcctggactc aagtgcgtcc tgtcatggga aaccaggact cactatcttt tggggctgct
    10381 ggagaaagga aggatggtag tctccgaact ttcgggagcg cgtgtcttcg cattgcatgt
    10441 ccatgacgat tctatggaac ctctgttccc tgaaggagag atcatctttg taaaccccga
    10501 ttttgaactt cagcctggcc attatgggat cttcttggat caaacgggcg gggcggatcc
    10561 tggttgtctc agacagttca agaagattca ggagcgatat gtcgtgcatt cactcaacat
    10621 ccaatacaag gacacgcctc tgacgagtca ccagaaggtt attggacgag tgatccggct
    10681 tcgcatgaaa ctgtaagctc tttgctggac gggattgatg gccatatctg atcaatgatg
    10741 attcgtggaa atgggggaag accagctgac aggattgaat agcaggaagc ctcaggcata
    10801 acgtgaaatc aaattcctgt gtcgaacgcc atgagattgt gcagctcttt gagaacaact
    10861 aacatcaagg tatccaatga gcttcaccaa gcggagacca gagccgggtg gagtggaaca
    10921 gacggcagag ggctcatagg cggatctgtt ttaaaagtga gtgaacatca tgacgatcgt
    10981 cccggataaa tagaaacata agatacgcat gtgtatctgc cagattagga accaacactt
    11041 gacatggtgt tgagaaccca cgggaacatg ttgccaataa tgccgactta gcgattccgt
    11101 tcttcattgg accgctatgc atgtcacaag gagagtgcca tgttggaatt gtatcagttc
    11161 gaagaatgtc cctacagtac aaaggtgcga aaaaagctct ctgaatggga gatcgattgc
    11221 attctcagaa acgtctcgca ggacaaatcg aagcgaagtc ttttgtggaa aatttccggc
    11281 caagatgagg tgcccacact catcgattct gatcgcgacg cgattattgc tggtgatgaa
    11341 aaaagaatta ttcaacatct cgaaacttac tatgctcgga gatccacata gaccagggta
    11401 tggcgtgttt tgttcgtggt cctgttatgg cgctctcgta gtctctttaa aaaaaaaggg
    11461 tatcgtcatc tttttaatca ggacattcat tgggaaaaat ctgattatgc acggtaggag
    11521 gggctattat gaagggaact caacaatata cggccaaaga atttaacctt tcaggattaa
    11581 aaggtatttc agataaaacg cttgagatgc atttcaaact gtatgaagga tatgtcaagg
    11641 cgacgaatac gctacataaa ctcattgcca atattttaac agacgggaag gtggatcagg
    11701 aggaagtgcc agcttattcg gagctcaccc ggcggctggg atttgaatat aacgggatgg
    11761 tcctgcatga gttatatttt gaaaatatga cctcccaagc atcatccggt ttcaaaggtc
    11821 aggattcctt agggaaagcc ataacggaga gttttggaag ttttgagaat tggaagacgg
    11881 acttttctag catagggaaa atgcgaggag tcggctgggc catttgctat caaaaccctc
    11941 ataacgggcg attatccaat cattgggtga gtctccatga atgcggtaat gtcgcaggct
    12001 ttcgacccct gttggtgatg gatgtatggg aacatgccta tctgctcgat tttcaaccgt
    12061 ccgaacgggg ggcatatatt gaaagctttc ttttgaatgt caattgggat gtcgtggcta
    12121 aacgccaata gacatctgat aggagaacat gtgggaatgg gaattgtgca acattacctt
    12181 gcggaggacc acgcgcggtt agataatctc ttgcagcggg ccattgacaa acaggagaaa
    12241 attgaccacc aggcctataa agaattccgc aggggtcttc tccggcacat tgcgatggag
    12301 gaaaaggtgc tgtttccaac cattcaacga ctgcgaagcg gcaccccact tcccttgacc
    12361 gacaagctcc ggcttgatca tggggccctt ggcacgttga ttatgccgac gcccacgcga
    12421 gccatcatag agttaatccg cacgatcctt gtttctcaca atggcctgga ggaaggcccg
    12481 gaaggggtgt atgcgcaatg cgaacaggtt gccggtgtcg ggatcgagga tctcttccgg
    12541 cgtttgcagg ctgttgcgcc ggtatcagta gccgcctatt ccgattcccc cacagtgttt
    12601 gcgacaatac gccgtgttgt aatgagagcc ggttatcccg cagagtctat ggaatttcat
    12661 tgaaggggac aaccacagtg atggattgtc cggcacgatc gatttctgac gcgtattcct
    12721 cggttgcggt ctcataccca tttcatacaa atgccgatca cctgaatacc atcaatttcc
    12781 taaccaccag tctcaaagta tcgtccgggt gatcaagtgc atacaagttt tttcagagct
    12841 attgtcaaat gtagtgtctg agccgttgct tacgcggcga ccgagtccgt ggctgcttgt
    12901 tcaagcccat caataaattg gatgcccgca acgactttga gcaattcgtt gaatcctcgc
    12961 aagcgccgcc aggttttctc cgcacactgc cccgacttaa acatcatgtg cagcctgcca
    13021 tcccgggtga gacagccctt ggtccgtgcg gtccgatggc gaattgtccc aaaggtcgat
    13081 tcgatgggat tggttgtccg aagactcggc caatgggcag caggaaaggc gtagaaggtc
    13141 agcaacgcgt cctgatcttt ctgcaagcag gcggtggcct tcggatactt cacgtcatac
    13201 gtgttgatga agacatcgaa cgcgtcctct gcggtggctt tagttggggc ctgccagatt
    13261 tcatgtaagg cctgtttcgc cttcggctgc acacttttgg gcaaggcatt caagacattc
    13321 ctcgtcttat ggcaccaaca ccgttgctgt tgcgtgcttg gatatatttc ttccaaggcc
    13381 ccccagaagc ccatggcccc atcgccgatg gccaaggccg gagcggtcag ccctcgcgct
    13441 ttcagcgtta acaggacttc ccgccaactc tgggtggatt cgcgaatccc gtcttcgatc
    13501 gccaacagat gtttctcccc gtgggcattc atgccaatga cgaccaacgc acacaaccgc
    13561 tgcgtctctg ctcgtaagcc actatagatg ccatccaccc agagatagac ccactggtcc
    13621 gcatccaggc gccggtgccg ccaggtttca tattcctccc gccagccctg cttgagccga
    13681 gccaccgtac tggcagacag ccccttggcc tccgggccca ctaacgcttc gagagccatc
    13741 tgcatctccc ccgtcgaaat gcctttcaaa tacagccacg ggattgccgc ttccaggctg
    13801 gctgtcttac gcacatacgg cggcaccaag gccgaatgaa acctcactgg gtcgccgtgg
    13861 cgacttcgca ctttgggtac ccgtaccgtc acgggcccca tgcccgtttg aatagcgcgt
    13921 tcgggatgat ggccactgcg gacaaccctc gcatgcccct gctcatcccg ttgatccgca
    13981 taggtggcca agagttcagt cacctccgcc ttcagcgcct gcgcaatgag ctggtgggcg
    14041 cctgcccgaa taatgtccat gagcgaatcc ttgttggctc gatcttcttc ctctgttttg
    14101 ctacgctttt gcatcgtggc gtctctcctt tgtggttggg atgtctcgca acacccatgg
    14161 caacccgaga cgccgcgtct tttcaacaca ctccatgcac tactttcgaa tataactccc
    14221 ggacaatttg acaccgtctt aagtggatac gtaaccctgg ttcaatcatc ttcacgtcat
    14281 tcaccggagc gtatcctgag tcgacgaatc agacgcaatc acaaccccgt gttcaaggcg
    14341 aacgtggcgc tggctgccct gacgggtgat ttaactaccg ccgaggtggc gaagcaattt
    14401 gaggtccctc caacccagat cgctcaatgg aaagcacagc tcctggagca tgcccccgga
    14461 gccgtcaatg agaagcggca cgagacgcct cctgccgtcg atatcaaaac cctgcatgcg
    14521 aagatcgatc aacggacgct ggagaatggt tttttagggg cgctcggcaa ggccggactg
    14581 ctgagcgtaa aaccatgatc gaccggaccc accaactccc cgtctcgcac caagcgaagg
    14641 gactcggatt gtcttgaggc tgtctctacg accaacccgt gccagtcaat gagagtgaac
    14701 gggcctttat gcatcgtctg gataaactgc atctggcaca tccattcgct gggtcgtgga
    14761 ggttgagaga ttggcggcgc ttggagggct tccgcgttgg gcggaagcat ggcagaacac
    14821 tgatgaagcg tctgcgcctc gaggcgctct attgcccgcc caggacgact caggcgcatc
    14881 gaggacacgc cgggtacccg tacttactca ggaatttgtc cattacgcgc cccaatcagg
    14941 tatgggcctc agctgtcccc tacatcccga tggcccatgg atgtgtcgat ctgaccgtga
    15001 tcctggactg gtctagtcgc cgagctctgg catagcgcgt agcggtgacg atggatgggg
    15061 aatttatcat ggatgccttg gaagaggcga ttgagcacta tggctgtcca gccatcatga
    15121 accccgatca cggccgccaa tataccagtg aacgctttac tcaagcggtc aaggatcgag
    15181 acattcaaat tagcatggac agtaaaggag cctggcggga caatgttttt gttgaacgac
    15241 tgtggcgctc agtcaaatat gaggatattg cctggcaggc cgatgacacc ccgcctgagg
    15301 tccaacaagg actgacccgg tattttgcct tttacaatac cagtcgccca cttcgggcac
    15361 atactgggcc gacaccagat acggcgtagt tcacccagtt gccaatagcc gaagtggcat
    15421 aattcattgg gttacggtcc acttaagaat tggcttggga tgttcagata aaggggacca
    15481 cccctttcgg ccttcagtgc atccaccgag tagttacatc atgtttttaa aagatttccg
    15541 ccggcatttc ttgttattca gtaatcgaat ccaatagttt ggccaactcc gcagtcctgg
    15601 cttgacgaga atgcttgatg acttccgcct tatttggaat tggcgcgtgg ccttcgcgaa
    15661 tcagggagag gaatttaaca agtccacgca taatttgctc cttggaatct aagggggcga
    15721 tcgttgagat accagcggca gttaacacat tcgcggtatc tc
//
LOCUS       DATIAL010000515        10109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_629132, whole genome shotgun sequence.
ACCESSION   DATIAL010000515 DATIAL010000000
VERSION     DATIAL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629132"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1168)
                     /locus_tag="VLA60_15875"
     CDS             complement(32..1168)
                     /locus_tag="VLA60_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HSF10890.1"
                     /translation="MLCNAAVTSCVNGQAASGLRAAAGTEIMSYRDALHWTMHDALER
                     HDSVLILGQGVDDHKGIFGSTTGLSQEFGADRVMDTPLAEEGMTGVAIGAALNGLYPI
                     QTHIRADFSLLAMNQIINLAAKYKYMFGGRFDVPMLIRMIVGRSWGQGAQHSQSLQSL
                     FAHIPGLQVVMPSSSQSILDTYPAVIDRYRGPVISFEHRLMYDLQFHVTPLATRQEID
                     PFGSRLVRPGEQVTIVATSIMVLEAMRAAEHLALVSDIQCEVIDLHCVSSPDWDLVMH
                     SVEKTGRLVVADTSWREYGVCAEVCRVVAERNPGCLKTPVVTLGMQPAPCPTAKCLED
                     LFYPDLRQLVDALAVLITGKENHELPLPDNQSMADVYKRFKGPF"
     gene            complement(1158..2087)
                     /locus_tag="VLA60_15880"
     CDS             complement(1158..2087)
                     /locus_tag="VLA60_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSF10891.1"
                     /translation="MENSQKFCELFFQALRIRLVEERIIELYPGDAIQSPVHLSIGQE
                     AVAVGACRDLKKTDLVFCTYRSHAFYLAKGGSLAEMFAELYGKVTGCARGKAGSMHLA
                     APEVGLMGASAVVASTIPHAVGAALAAKRLGHDQVVVGVFGDGATEEGVYHESLNFAV
                     LHRLPVIFLCENNGLAVHSSQKSRQGYDILDHARSYRLPVYHIAQGYDFVKVADTMSD
                     IIQRVRLTREPVFVEIRTYRYKEHVGPGEDFMCGYRQPEEWLAWKRQDPLEQYGNLVE
                     TFRPSILKEIDEAVHFAMSSPFPSVEELLRDVV"
     gene            complement(3400..3645)
                     /gene="csrA"
                     /locus_tag="VLA60_15885"
     CDS             complement(3400..3645)
                     /gene="csrA"
                     /locus_tag="VLA60_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HSF10892.1"
                     /translation="MLILTRKIDEAIRVGDDIRIVLVQIKGGQVRLGIECPSHVRVLR
                     EELYEAVRQENLNAVSSDPKHLAHLPRPTRPPVKPTE"
     gene            complement(3754..4230)
                     /locus_tag="VLA60_15890"
     CDS             complement(3754..4230)
                     /locus_tag="VLA60_15890"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HSF10893.1"
                     /translation="MKIQTSRFGMLDVSDDTLLTFPFGLVGFPAFRRYVVLDPPEDAD
                     YRWFQSVDEPSLAFVIMDVHLLQPDFRTNLSEEGLAELGMTQTDSISIMAVVTIPSDQ
                     PDQATANLRAPLVVNERTRHGKQLILHESIPLRYPLFQDSTQCQSYSGAAREAASV"
     gene            complement(4318..6672)
                     /locus_tag="VLA60_15895"
     CDS             complement(4318..6672)
                     /locus_tag="VLA60_15895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10894.1"
                     /translation="MASILVINDDPVQLHLLASWLEKDYFAVNRFVCSEEAWRWLQEG
                     NVPDVIVLDLHMPGISGWRFCELLHSFFGSTQAVPPVLAVSATYTGIDAQDILKDLGA
                     SAFLALPTDPQRFRQQVRQLVESPPSPQRPQIWIVSANNSAIQGIHHLFVERGWHVVE
                     WEAGKQVQVAVNLVDPDIILIDDPLPDTTSEEFGIWCKGQFPQVMCIVLGHESTAGRS
                     FPEAIHVDAYLPKACDPFNIISLCEKGRWERAFTRVEHLLDIRTDDLRESEAQFRELF
                     ETLPDILVIYDHRGIITHVNSLGAQQLGYRPPVLMGKTFSLIRPESADAESSPTVSNP
                     ARGGRRWEETFLRQKNGSNLPVEMLERMVQFQGERQTLFIARDLTVRKHMEEEKFALE
                     HQLRQVQKMEAIGRLASGVAHDMNNTLTAIMAHASLLKIQDKAGTPLWTASDVIEKAV
                     RRGKELTSQLLGYARQGKHHHVTVDTHEVIQEVMSLLGRTIHKLITFRADLSAARPHV
                     VGDPNQLYQILMNLGVNACDAMGERGELLVQTSNETVTPEYASRVPGLCAGDYVLIQV
                     TDTGTGMSLETQRHIFEPFFTTKEQGQGTGMGLAMVYGIVKNHHGYIGVTSSPGCGTT
                     MRVYLPDAPCATHKTRSTQKTEPSHGTGHILVVDDEKDVGEAAQAILEYLGYQVTVVL
                     NGKEAVEICQDLTIPVDVVLLDMVMPIMSGAACFEELRRIRPDLKVILCTGYDRNHAV
                     QDLFNQGVIGFIQKPYDVDELAHACQVVLTNDNRVQPCGTGRTC"
     gene            complement(6675..7646)
                     /locus_tag="VLA60_15900"
     CDS             complement(6675..7646)
                     /locus_tag="VLA60_15900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSF10895.1"
                     /translation="MRILVVDDDPLTLHMVVYRLRQWGHDVISCTDGDSAWKVLARGS
                     VPNVAIVDWIMPGINGPELCQRIRGRTDCPYVYIVMLTGRNNPEDLIAGLDAGADDYL
                     TKPFHLGELDARLRAGKRIVDLQNELISARETLRIQAMQDPLTQILNHGAIVDSLLRE
                     IDRAHREQQPLSLILADLDGFKNVNDSYGHVAGDQVLVEVARRMRFCLRSYDAIGRYG
                     GEEFLMVLPNSDAAQAVRLAERIRVAISQKPFRVHNTDLTVTVSQGVTTWTDPCPIPI
                     EQLIQSADGVLYLVKNSGRNGVEFAQFHGHADETFSRLTSSPTSMKQ"
     gene            complement(7716..8867)
                     /locus_tag="VLA60_15905"
     CDS             complement(7716..8867)
                     /locus_tag="VLA60_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HSF10896.1"
                     /translation="MRVTDRQQVDALLRAIQSIQRNIGDRQGQISSGKRVNRPSDDPA
                     ASERINQFRNVLRTTEHRLNTVNEGMGRLNLSDSVLEQAGNTVQRAKELALNMASDTN
                     TSVERRNAAQEVQQLILGLAGIANTQLNGRFVFAGSQTKTEPFVPGSATGSASITNGG
                     GASVGVSVASASVLQPDAYQIQFTSSTQFDVLNLTTNQTVSSGNTYTSGAPFSFDGLD
                     VTISDGGGPPATGDQFFIRVGYTYQGDGAAVEVEVGDGRTVESNVPGDRIFSGPEVDL
                     FHNLQDFHQALVTNDVSGIQAAIGQFDPALSQVNDARATIGARVNRLDTIKDGLDLLS
                     VNTQTLRSDFEDADIAQVASDLATLQNTLEASMSTLARQFQTNLLDFIK"
     gene            complement(8949..>10109)
                     /locus_tag="VLA60_15910"
     CDS             complement(8949..>10109)
                     /locus_tag="VLA60_15910"
                     /inference="COORDINATES: protein motif:HMM:NF044995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HSF10897.1"
                     /translation="IARHVDTINGLATKIASLNDVIFKTESSGRDALDLLDQRRVLIN
                     DLAGLVNIEQVPLNDGIGINVGGQLLVAGNHANALSTEADPDNPPMHDVTFVRSDGSE
                     LSISHNIHGGQIGGLLSQRDGDMVKFQDQVDRLAAVLINEFNQQHQVGYGLDGTTNNN
                     FFSTLSPQAPLAHDRNTGSATGSSVTIADPTLATFQGYEVQFSGASAYLVVNTATGVT
                     VTSGAYTSGSPLSFDGLDVVINGTPAAGDVFYVNAQKGAAQQFGVALSDTDKIAAAST
                     AAGIPGNNTNALNLVAIHTNRHVDLGNVTLNDYHTITIGDVGSATQESTQALHSKRLE
                     VDQLTALRESVSGVSLDEELTNLLSFQRSFEASARMITVADELMQTMLAMGR"
BASE COUNT         2581 a         2815 c         2295 g         2418 t
ORIGIN      
        1 ttcctttaat gaggtggtcc gacaatcccg tttaaaatgg gcccttgaac cgtttataca
       61 catctgccat tgattggtta tcgggcaagg gcaactcatg gttctccttc ccagtgatga
      121 ggacagccaa cgcatccact aattggcgca aatcgggata aaagagatct tccaaacact
      181 tggctgtagg acagggagcg ggctgcattc ccaaggtcac caccggcgtc ttcaaacatc
      241 cgggatttct ttcagcgacc acacggcaca cctccgcgca tacaccatat tcccgccaac
      301 tcgtatccgc cacgaccaac cgtcccgttt tttcaacact gtgcatgacc aaatcccagt
      361 cagggctgga cacacaatgc aaatcaatca cctcgcattg aatatcggac accagtgcca
      421 aatgttctgc tgctctcatg gcttccaaca ccatgatgga ggtggccaca atggtgacct
      481 gctcacctgg tcgcactaac cgggacccga agggatcgat ctcctgccgg gtggctagag
      541 gagtcacatg aaattgcagg tcgtacatca accggtgctc gaatgagatg acgggacccc
      601 gatagcgatc aatcaccgcc ggataggtgt cgagaatcga ttgcgaactc gagggcatca
      661 caacctgcaa cccggggata tgcgcaaaaa gagattggag gctttgtgaa tgttgcgcac
      721 cttgccccca acttcgccca acgatcatgc gaatcaacat gggcacgtca aaccggccac
      781 cgaacatata tttgtatttg gccgctaaat taattatctg attcatggct aacaaagaaa
      841 agtcggcacg aatatgggtc tgaatcggat acagcccatt taacgcggcc ccaatggcta
      901 cccctgtcat gccttcttcg gcaagtggcg tatccatcac tcgatccgcg ccaaactctt
      961 gactcaatcc ggtggtggaa ccaaatatcc ctttgtggtc atccacaccc tgtcccagga
     1021 taagaacgga atcatgccgc tctaaggcgt catgcatggt ccaatgcagc gcgtcccgat
     1081 agctcatgat ctccgtgccc gccgccgcac gaagacccga cgccgcctgg ccgttcacac
     1141 aggatgtgac cgccgcatta cacaacatcg cgaagtaatt cctccactga gggaaatgga
     1201 ctgctcatgg caaaatgtac cgcttcatct atttctttca ggatggatgg gcgaaacgtt
     1261 tcgactaggt ttccatactg ttcgagagga tcctgtcgtt tccaagccaa ccactcttca
     1321 ggttgtcgat agccacacat gaagtcttca ccaggcccga catgctcttt gtaccgatag
     1381 gtccgaatct ccacaaatac cggttctcgg gttaaccgaa cacgttgaat aatgtctgac
     1441 atcgtgtccg ccaccttcac aaaatcatat ccctgggcaa tgtggtacac cggaagacga
     1501 tatgatcgtg catggtcaag aatatcgtac ccctgtctgg atttttgtga tgaatgcacg
     1561 gctaatccgt tattctcaca cagaaatatc acgggtaagc gatgaagaac cgcgaagttc
     1621 aatgattcgt gatagacgcc ttcttccgtg gctccgtctc caaacacccc aaccaccacc
     1681 tgatcatggc ccaaccgttt cgccgccaaa gcggcaccca cggcatgagg aattgtactg
     1741 gccaccacag ccgaggcacc catcaaccct acctcgggtg ccgctaaatg catcgaacca
     1801 gcctttcctc gcgcacaccc ggtaactttt ccatacagtt cggcaaacat ttccgccaaa
     1861 cttccccctt tagccagata aaaagcatgg ctacggtagg tacaaaagac cagatctgtt
     1921 ttttttaaat cccgacatgc ccctacagca acagcctcct gtccaatcga taaatgcacc
     1981 ggactttgaa tggcatcgcc aggatataat tcgataatcc gttcttccac caggcgtata
     2041 cggagcgcct gaaagaacaa ttcacaaaat ttttgagaat tttccatatt gttttgcgat
     2101 tatccctctg cctcgatcac cggtttcaga acaggcatca cagaccgtga aaagcttcac
     2161 gaccttttca tacgatgtaa agaagttcaa gttttatgcc atgcagttca agttacaaca
     2221 gacgggatcc agctcccaat gctggcaaat tccaggttat tcgaaaaact tttacctact
     2281 aggaattcat tgctgacaaa ctcggaattt tttgcctagc aagagaaacg ctgattgaac
     2341 gcagggctgg agaaaccggt gagaccacat tgagacatgg cctttatgac gctatgaaat
     2401 aaaaccgaat acgacctact cgtgcgacga taatcctcaa tcctctttca atcaaggcct
     2461 atgtagaatc catcctcgta ctggcttccg gtgggttaac acgatctcac acactgacac
     2521 tgtaagggtg ttcccctcct tgggtaatga aacccctgaa gatctccctg gggtaagacg
     2581 gacaggagga ctgccctgag gatacccaaa cgcctcagag gaaatggtcc ggcctgaagg
     2641 aaggcctcga attaagtctt ggcgcagccc cagagcacat gccttgcagg cgatgcgaac
     2701 agatgccctc atcgggtgcc cgcagatgga tatcccagca atcacggtct cggttgtggt
     2761 cttgatggtg ttccctggga tggccggaag atgacagccc cggtgtgcgg ccgcctgatg
     2821 ccgggggccc tgccacagtc tttttcccta tcgatcactc ccggcgtggg cagacacggt
     2881 gttagccttg agcatccggt cgattggctg tcatggttac caccttgtgg atcaggactg
     2941 actgcatatc cacactgaca tgttccctct ccccgtacca agacttcttc ttgcctttgc
     3001 acccaatacg ggccgccact tttggcaaga cggcattatg gccattttca attttctgtt
     3061 tgccgcgact aagtgcgttc ttttcacaag gtgatttcgg aaagcaaatg ggtcgcatct
     3121 acaaagggca acacttgatt cctcacaacc agacaacttt gaattgcgcg cgcatcgtgg
     3181 caaccagatg agagagccgc gtcgggccca tgaggtgcgt ttatctactc cgacccaata
     3241 cctgcccaca cttttaaaca agccacctga accactcgtt ttgccgatcc ctgccagaat
     3301 ggcaggggca aatgtttagg cggagcccac aatgcggtgg atggacccga tgaggaatcg
     3361 ccacacatga cgaagaacac gagcctgatc ttattaggtt tattcggtag gtttaaccgg
     3421 cggccttgtc ggtctgggta ggtgagccaa gtgcttcgga tccgaggata cggcattcag
     3481 attttcttga cggacggctt catataattc ttcacgaagg acacggacat gcgacgggca
     3541 ttcaatcccg agtcgcactt gccctccttt tatttggacg agaacaatgc gaatgtcatc
     3601 tcctacacga atggcttcat caatttttct ggtgagtatg agcatggtgc cttccttggc
     3661 tttgaggtaa aattcacgcg gagaagaacc gtcctgacat cataaacgca ttaaaccggt
     3721 tgtccctgtg gaccggtagt gtgcgtgcat tcattacacc gatgcggcct ctctcgccgc
     3781 cccggagtaa gattggcact gggtagaatc ttgaaataat ggatagcgca aggggattga
     3841 ttcatggaga atcaattgtt tcccgtgtct tgttcgttca ttcacgacaa gaggggcacg
     3901 aagattcgca gtggcctgat cgggctgatc agaggggatc gtgacgacgg ccatgattga
     3961 aatggaatcg gtctgagtca tgccgagttc cgccagtcct tcttcggata ggttggtgcg
     4021 aaaatccggc tgcaaaagat gcacgtccat tatcacaaaa gctaagcttg gttcatccac
     4081 ggactggaac caccgataat cggcatcctc tggcgggtcg agcaccacat accgtcggaa
     4141 agctgggaac cctacgaggc caaatgggaa cgttaaaagt gtgtcatcgg acacatccag
     4201 cataccgaac cgactagtct gaattttcat gttgggatgt tctccacata ggtcaatatg
     4261 ttcatcttcc tgctgaagtg agcctgtcct cgagttccct ctcaacaaaa tctatgctta
     4321 gcacgttctc cccgtaccac agggttggac ccgattgtca tttgtcagaa caacctgaca
     4381 ggcatgagcc aattcatcga catcatacgg tttttgaata aacccaatga caccttgatt
     4441 gaataaatct tgtacagcat ggttccggtc ataacccgta cagagaatga ccttgagatc
     4501 tggtcgaatc cttcgtaatt cttcaaaaca tgccgcccca gacattatag gcatcaccat
     4561 atccaacagc acaacatcca caggaatcgt caagtcctga caaatttcca cggcctcctt
     4621 cccattcaac acaaccgtca cctgatagcc gaggtattcc agtatagcct gcgcggcttc
     4681 ccccacgtcc ttctcatcgt ccaccaccaa aatatgtccg gtcccgtgag aaggctccgt
     4741 cttctgagtc gatctggtct tgtgagtcgc acacggtgca tccggaagat agacacgcat
     4801 ggtcgttcca catccagggg aactcgtcac cccgatatat ccatgatgat ttttgacgat
     4861 accatacacc atcgccaatc ccattccagt tccctgccct tgctctttcg tggtaaagaa
     4921 tggctcaaaa atatgtcgtt gagtctctag tgacatcccc gtcccggtat ccgtaacctg
     4981 aatcaacaca taatcacctg cacacagtcc cgggacacgt gaggcatatt ccggtgtgac
     5041 tgtttcattc gaagtctgca cgagcaattc cccacgttca cccattgcat cacaggcatt
     5101 gacgcctaaa ttcatgagaa tctgatagag ctggttagga tcccccacca catgaggcct
     5161 cgcagccgac agatccgctc gaaatgtgat gagtttatgg attgtccttc cgagcagact
     5221 catcacttcc tgaatcactt catgggtatc caccgttaca tgatgatgtt tgccctgcct
     5281 ggcatacccc aggagctggg aagtcaattc ctttccacga cgaacggctt tctcgatgac
     5341 atcactcgct gtccacaagg gggtgcccgc tttatcctgt atcttcaaca aacttgcatg
     5401 ggccataatt gccgttaacg tattattcat atcgtgtgca acccctgagg ccagtcgacc
     5461 gatagcttcc attttttgaa cctgccgcaa ctgatgttca agggcaaatt tctcttcctc
     5521 catgtgcttt cttaccgtca aatcgcgggc aatgaaaaga gtttgacgtt cgccttgaaa
     5581 ctgaaccatt cgttccagca tctctactgg aagattgctg ccgttttttt gacgaaggaa
     5641 cgtttcctcc catctccgac ccccacgggc agggtttgaa accgtaggac tggattccgc
     5701 gtctgctgac tcaggtctga ttaacgaaaa ggttttcccc atcaggacag gcgggcgata
     5761 ccctaattgt tgtgctccga gggaattgac gtgagtgatt attcctcgat gatcataaat
     5821 caccaatata tccggaaggg tttcgaacag ctccctgaat tgagcttcag attcgcgtaa
     5881 atcatcagtg cggatatcca gtaaatgttc cactcgagtg aatgcgcgtt cccagcgccc
     5941 tttttcacac aatgaaatga tattgaatgg atcgcacgct tttggtagat acgcatctac
     6001 atggatcgcc tcaggaaagg atcttcctgc cgtagattcg tgtccgagga caatacacat
     6061 gacctgagga aattggcctt tgcaccaaat cccgaactct tcgcttgtcg tatccggcag
     6121 aggatcgtca atgaggataa tatccgggtc aacaagattc accgccacct gaacctgctt
     6181 ccccgcttcc cattccacca cgtgccaccc ccgttcaaca aagaggtgat gaatcccttg
     6241 aatggccgaa ttgttagccg aaactatcca gatctgtgga cgctggggac ttggaggact
     6301 ttctaccagc tggcgtacct gctgacgaaa gcgttgcgga tctgtcggaa gggcaagaaa
     6361 tgcggaggcc ccgagatctt ttaaaatgtc ttgagcatcg attccggtat atgtggcgga
     6421 aacggccagg acaggcggca ctgcttgtgt cgacccaaag aaggaatgca ataattcgca
     6481 aaaacgccat ccgctaattc ccggcatatg aagatcaaga acaatgacat ccgggacatt
     6541 accttcttgg agccaccgcc aggcctcctc agaacagacg aaacgattga ccgcaaagta
     6601 atccttctca agccaactcg caaggagatg gagttggaca ggatcatcat taatcacaag
     6661 aatggaggcc ataattattg cttcattgaa gttggggaac tcgtgaggcg cgaaaaggtc
     6721 tcatccgcat gcccatgaaa ttgcgcaaac tccacgccat ttcgaccgct atttttgact
     6781 aaatagagca caccgtctgc agattgtatt aattgttcaa tgggaattgg acagggatcg
     6841 gtccacgtcg tgaccccctg actgaccgtc accgttagat cagtattgtg aacgcgaaag
     6901 ggtttctggg aaatcgccac tcgaatccgt tcagcgagcc gcacggcctg ggccgcatca
     6961 ctattgggca acaccatgag gaattcctct ccgccatatc ggccaatggc atcataggaa
     7021 cgcaaacaaa aacgcatccg tctagcgacc tcaaccagca cctgatcgcc cgccacatgc
     7081 ccataggaat cattcacatt tttaaatcca tccaaatcgg ccagaatgag actcaagggt
     7141 tgctgttccc gatgcgcccg atcaatttcc cgcaacaacg aatccacaat ggcaccatga
     7201 ttcaggattt gcgtcaaggg atcttgcatc gcttgaatcc gaagggtttc ccgagccgaa
     7261 atcaattcat tttgtaaatc gacaattcgc ttccctgctc gtaaccgtgc atccaattcc
     7321 cctaaatgga aaggctttgt gagataatca tccgctcccg cgtccaatcc ggcaatgaga
     7381 tcctcaggat tgttccgccc cgtcaacatg acaatataca catagggaca gtcggttctc
     7441 ccacggatcc tttgacacag ttcagggcca ttgatccccg gcatgatcca atccacaatc
     7501 gccacattcg gcacagatcc tcgggccaac accttccatg cagaatcccc gtcagtgcag
     7561 gaaatgacat catgtcccca ttgccgcaat cgatatacca ccatatgcaa tgtgagggga
     7621 tcatcatcaa caacaagaat tcgcataaag atttcgtccc tattcaatca cccggtgaca
     7681 caatgtagtc caataaaata agaggccttc acggcctact taataaaatc taataaattc
     7741 gtctgaaatt gacgcgccag ggtgctcatc gacgcttcaa gcgtgttttg tagtgtagcc
     7801 aaatcagaag ccacttgagc gatatcggca tcctcaaaat cggaccggag ggtttgcgta
     7861 ttcacactca gcaggtctaa accatctttg atagtatcca gtcgattgac tctggcaccg
     7921 atcgtggctc tggcatcgtt tacttgggag agagccggat caaactgacc gatggccgct
     7981 tgaatacccg acacatcatt ggtcaccaag gcctgatgaa aatcctgaag gttgtgaaac
     8041 agatccacct ctggacccga aaagattcgg tccccaggga cattgctttc cacggtccga
     8101 ccatcaccaa cttccacctc caccgccgca ccatcgcctt gataggtata gccgactcgt
     8161 ataaaaaatt ggtcgccagt ggcagggggc ccgcctccat ccgaaatggt gacatccaag
     8221 ccatcaaacg agaatggagc tccggatgta tacgtattgc ctgaggatac ggtctgattc
     8281 gttgtcagat tcagcacatc aaattgtgtc gaggaggtaa actggatttg atatgcatcc
     8341 ggttgtaata ccgacgcgga cgccacagat acgccaacac tggcacctcc cccattggtg
     8401 atactggctg atcccgtggc cgaacccggg acaaacggtt cggtttttgt ttgactccca
     8461 gcaaacacaa atcgtccatt aagttgagta ttggcaatgc cggccaagcc caggatcagt
     8521 tgctgaactt cctgagccgc attccgtcgc tccacggacg tattcgtgtc gcttgccata
     8581 ttcaaagcca gttctttcgc ccgctggacg gtattcccag cctgctccag gacgctatcg
     8641 gatagattga gccgtcccat tccttcattg acagtgttca aacgatgttc agtggtccgt
     8701 agaacattcc gaaattgatt aatccgttcg gatgccgcag gatcatcaga agggcgattc
     8761 acccgctttc ctgaagaaat ttgcccctgg cgatccccga tattacgctg aatactttga
     8821 atcgccctta gcagcgcatc aacctgttga cgatcagtca cacgcatacg atagttcgtc
     8881 cttatccgaa agatttcgac cgctcatcaa tgatgacttg ccctcagatg attgcctcat
     8941 caagaaagtt aacgacccat cgccagcatc gtctgcatca attcatccgc taccgtaatc
     9001 atccgggctg aggcttcgaa tgagcgctga aaacttagga gattggtcaa ttcctcatcc
     9061 aacgaaaccc cggagacact ttctcgcaaa gccgtcaatt gatcaacttc aagccgctta
     9121 ctatgcagcg cctgtgtcga ttcctgggta gcactcccga catccccaat cgtaatcgtg
     9181 tggtaatcat tgagtgtcac attcccaaga tccacgtgcc gattcgtatg aatcgccacc
     9241 agatttaatg cattggtgtt atttccggga attcctgccg ccgtagaagc ggccgcgatc
     9301 ttatccgtat ccgagagggc cacaccaaat tgctgggccg cgcctttttg agcattgaca
     9361 taaaacacat ccccggcagc cggcgtgccg ttgatcacga catctaaccc atcaaaactc
     9421 aagggtgatc cggaggtata cgccccagac gtcacggtca caccggtcgc cgtattgacc
     9481 accaaatagg cagaggcccc ggaaaactgc acctcatacc cctgaaaggt cgccagagtg
     9541 ggatctgcaa tcgtgaccga actcccggtg gcacttcccg tattccggtc atgggccaga
     9601 ggagcttgag gacttaaggt ggagaagaaa ttattattcg tggtgccatc taaaccatac
     9661 ccgacctgat gctgttgatt aaattcatta atcaacacgg ctgctaatcg atccacttgg
     9721 tcctgaaatt tcaccatatc cccatcacgc tgtgagagta atccgccgat ttgtccacca
     9781 tgaatattgt gggagattga caactcactc ccgtcactgc gcacaaatgt cacatcatgc
     9841 attggaggat tgtccgggtc cgcttcagtc gacagcgcat tggcatgatt tcccgctacc
     9901 aagagttggc ctccgacatt aatgccgatg ccatcattca gaggcacctg ctcaatattg
     9961 accaaacccg ccaaatcatt aatgaggacc cttcgttgat ccagtaaatc cagcgcgtca
    10021 cgtccactgg attccgtttt aaaaatgaca tcattcagag aggcaatctt tgtggccaac
    10081 ccattaatag tatcgacatg cctggcaat
//
LOCUS       DATIAL010000516         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1597627, whole genome shotgun sequence.
ACCESSION   DATIAL010000516 DATIAL010000000
VERSION     DATIAL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597627"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..506)
                     /locus_tag="VLA60_15915"
     CDS             complement(261..506)
                     /locus_tag="VLA60_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HSF10898.1"
                     /translation="MGDPIIIAQRTPYVMDMEPGTYYWCRCGRSKTQPFCDGSHTGTE
                     FSPIEVELKEPQKVAWCGCKHSKKSPFCDGTHSRLES"
     gene            complement(554..1456)
                     /locus_tag="VLA60_15920"
     CDS             complement(554..1456)
                     /locus_tag="VLA60_15920"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HSF10899.1"
                     /translation="MSANAGCATSQANPPTFAVNHIYDVSRQQVTTFDDLLPQLLAAD
                     VIYIGEEHYTPSHLEAAQTILNALLAHDRHPALAMEMFSWDGQSGLDRYIQESDFTTE
                     QLIKNSQWNTNWGGEFADYKPLVTFAKSHKLKIFGLNPPRPLVRLVATKGLTEALQDP
                     EMQKWPIQEMVTDNPAYEQLIFEQIEACHPGLPKHVYRRIFEASIFRDEGMAQIIANY
                     LNTRSRTDGPLVSYTGGGHIQYKIPVPKRVERKHPGVKSITIYLEAWDPSKEQEVRSE
                     IQTGIADFLWLTPLGPKGLQPRCG"
     gene            1858..3165
                     /locus_tag="VLA60_15925"
     CDS             1858..3165
                     /locus_tag="VLA60_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10900.1"
                     /translation="MDDPRLGLAAFRETIKVSRESSPHHWEASRKCMVPETFQTMLPA
                     LIHHIQEVPLDPSLRDRLIDALQQFSTPTGSKEGNKVLKELTGFPPSKAVRALMVWGL
                     LADGGSRGNPEKPSGTQWEEIIRNVSNPYDALRHTATPSLLDIGAGDLSFEQELVDHY
                     IPYLRTQRTSLTLHAFDRLMPGSRVGGVYHKNLDRERYLQSYPPEELRFKFWGGMGLE
                     TFSKSKGRLPRYTVSTCHAPANPTFAYEPSRLAPEIIHAHLQSSRGNYHQSRYEGEPV
                     LEVSHQGRTLTFPDWKFDILGPLALLRFMAERSGVSILSAIDDEVFWEMLSQLLADDR
                     FRPINKIFTKALLPEIFGKVYEELSTMAVGERKDLSRLANLRDSMPFVGATKEDGQMS
                     CRFRYVEIRRGAVLEGVPSSFTARQFSRMQEESTPWWVILVTD"
     gene            complement(3524..3598)
                     /locus_tag="VLA60_15930"
     tRNA            complement(3524..3598)
                     /locus_tag="VLA60_15930"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3564..3566),aa:Gly,seq:gcc)
BASE COUNT          915 a          788 c          966 g          932 t
ORIGIN      
        1 tgggatagct ttccaataac aagatcagct acccacaatc gttgtggatt gtagtaaatt
       61 ttatttccgt gatgcaagcc attgactagt aaattgtaat tagatttttt atgctagttt
      121 ataatacata atttttcgat gaaatattga gtatgggtag caggcgaaga ccacggtatt
      181 gggctacatg aatgatacaa gcagggttaa cgcgattatt taaaaaatct ggctgaattc
      241 aataagccga gggccgtaat ttaggactcc agtctggaat gtgtgccgtc gcagaatggg
      301 gattttttgc tgtgcttgca cccacaccaa gccacctttt gtggttcttt gagttccacc
      361 tcgattgggg aaaattcggt tccggtgtga gatccatcac aaaatggctg ggtttttgat
      421 cgcccacatc ggcaccaata ataggttccc ggttccatgt ccatgacgta aggggttcgt
      481 tgtgcaatga taattgggtc gcccatgtgc ttggttctcc ttcattcttc aagaaatttg
      541 gtgttatata cagttagcca catcggggct gaaggccttt ggggcccaaa ggggtaagcc
      601 agagaaaatc ggcaatgccg gtttgtatct ccgaacgaac ctcctgttct ttggagggat
      661 cccatgcttc gagataaatg gtaatggatt tgacgccggg atgctttcgt tcaactcgct
      721 tagggacagg gatcttatac tgaatatgac cgccgcctgt gtagctcacc agcggtccgt
      781 cagtccggga tcgcgtgttg aggtaattgg cgataatctg agccatgccc tcatcccgga
      841 agatggatgc ttcgaaaatt cggcggtaca cgtgctttgg caagccagga tggcacgctt
      901 caatttgttc aaagatcaat tgctcatatg cgggattatc ggtcaccatt tcttgaattg
      961 gccatttttg catctctggg tcttgcaagg cttcggttaa tcccttcgta gcgaccaagc
     1021 gaacaagggg gcgtggcggg ttaagaccaa aaattttgag tttatgcgat ttagcaaagg
     1081 tcaccaatgg tttataatcg gcaaactccc caccccaatt cgtgttccat tgtgaattct
     1141 ttatcagttg ttcggtcgta aaatcggatt cttggatgta ccggtctaac ccggattgtc
     1201 catcccaact aaacatttcc atggccagcg ccgggtgccg gtcatgggcc aggagggcat
     1261 tcaggattgt ctgggctgcc tctaggtgtg agggggtgta atgttcttcg ccgatataaa
     1321 tcacatcggc ggctaatagt tgagggagca gatcgtcgaa tgtggtgact tgctgccggg
     1381 agacatcgta aatgtggttg actgcaaatg tcggcgggtt ggcttgtgac gtcgcacatc
     1441 cggcgttggc gctcatcacc atgattccca ggagtaggga aaggaagtgt tgtttcgcac
     1501 gtggaaaata atgggtgatc ataacgaggc aaggtgtagc acagttctct cctttgggca
     1561 atatttcgga tgccattcgg ttgtttcaga atattcagag cggctaaggt gcgtgaagaa
     1621 tcggatgagg gaactgtcag aatgtggcgg ttgaaaaatt caaatatttg gccttttcat
     1681 taacatgtga ggactgagac tcactggaag aaaatcatgg agtgttcaaa aaggttcgtc
     1741 cagcaaggcc gcagtcactt ggacgggcgg agcgtacgaa ggagtacgtg agcacggcca
     1801 agtggcgaga acgtcgctgg cgaccttttt caacacttcc acaagggatg acacattatg
     1861 gatgatccgc gactcggttt ggcggcattt cgggagacaa tcaaggtcag tcgggaatcc
     1921 tctccacacc actgggaggc aagtaggaaa tgcatggttc ctgaaacctt tcaaaccatg
     1981 ctgcctgccc ttattcatca tatacaagag gtccctcttg atccttccct tcgcgatcga
     2041 ttaatcgatg ccctgcaaca attctcgaca cccaccggaa gcaaggaggg gaacaaggtc
     2101 ctcaaagaat tgaccggctt tcccccttcc aaagccgtgc gcgcgctcat ggtatggggg
     2161 ctcttagccg atggcggaag taggggaaat ccggaaaaac cgtccggaac ccaatgggag
     2221 gagattattc ggaatgtctc aaatccctat gacgcgttaa ggcatacggc cacaccctct
     2281 ctgttagata tcggggctgg agatttgtcc tttgagcagg aattggtgga tcattatatt
     2341 ccgtatttac ggacgcaacg tacttcgctg accttgcatg cgtttgatcg gttgatgccg
     2401 ggatctcggg tgggcggcgt ctatcacaaa aatctggatc gtgagcgata tttacagagc
     2461 tacccgccag aggaattacg ctttaaattt tggggaggca tgggcctgga aaccttttcg
     2521 aagagcaaag ggcggttgcc ccgttacact gttagtacct gccatgctcc ggccaatcct
     2581 acctttgcct atgaacccag tcgcttggct cctgaaatta tccatgcgca tcttcaatcc
     2641 agccggggaa attatcatca gagtcgctat gaaggtgaac ccgttttaga ggtctctcac
     2701 cagggacgta ccttaacctt tcccgattgg aaatttgaca tacttggtcc cctggcactg
     2761 ttacgattca tggccgaacg aagtggtgta agcattcttt cggctatcga tgatgaggtc
     2821 ttttgggaga tgctcagcca gttgctggcc gatgatcgat tccgtccaat caataagatt
     2881 tttaccaaag ctcttcttcc ggaaattttt gggaaagtgt atgaggaatt gtccaccatg
     2941 gctgtcgggg agagaaaaga cctatcccgg ttggccaatc tgcgagattc tatgcctttt
     3001 gtgggtgcga caaaggaaga tggacagatg tcttgccggt tccgatatgt ggaaatccga
     3061 cggggggcag tgctggaggg cgttcccagc agttttacgg cacgccagtt ttcccggatg
     3121 caagaagaat caaccccttg gtgggtgatt ttggtgaccg actgaatgaa agaaataatt
     3181 gctgccctat gcgcggaaaa caggaaaaaa tacagtgatg agacccgtca gggcctacat
     3241 atcctttaag atagaatagg aaagaataga agtgacaaga gggcggaaaa aattaacttt
     3301 ctttatagaa aggcacgaac catgacagtc gggcaaatga aagattttct caaagatttg
     3361 aatgaggatt tagaaatatt ggtcaaaatt cccgggccaa ccaaggagga atgtctttgg
     3421 gagccactag acgggatgaa tataaaggtt gaacctcagc agcccggcaa atgaatggtg
     3481 aggaagtagg agtcgaaatg aaacggaaaa gacccgcttg aattggagcg ggaaagggga
     3541 tttgaacccc cgaccctcgc cttggcaagg cgatgctcta ccactgagct attcccgctc
     3601 g
//
LOCUS       DATIAL010000517         3293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_736681, whole genome shotgun sequence.
ACCESSION   DATIAL010000517 DATIAL010000000
VERSION     DATIAL010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736681"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..2294
                     /locus_tag="VLA60_15935"
     CDS             246..2294
                     /locus_tag="VLA60_15935"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HSF10901.1"
                     /translation="MGGGFAGLACARQLEKIWGTDTAKRVLLVSAENYFVYQPFLSEV
                     IGAGIEPRHVMNPIRLALRHCMVQRAEVAKLDLQHRRVHFSADEGVRLEPVEADHLIL
                     ALGSTTNLRAVPGMMEHGLFLKTLADALGIREHIIKRLEEADLESDSDLRRALLHFVV
                     VGGGYSGVETAGEILDLLNDARRFYPSFDPSELRVTLVHSGPYLLPELGEDLGSFAQQ
                     SLESRGMTIMLNRRASSVSASYIRLSDGTRLDSHNPICTIGNAPNPVLADLAAEYEKG
                     WLVTDEYLRLKGYTHVWGIGDGASNPDGYGHQAPPTAQFAIQLGRHASENIASSISGH
                     SLRPFRYKMMGQMATIGHHKGVCSIFGFRFSGFFAWWLTRTIHLLKLPGLDRKLRVMV
                     DWTFELFFPPDLNYLDLKKTQKIARMHVEPGDVIFRQGEQGSAFYVVEEGSLEAVRHN
                     KEGDVLWRDELFPGDHFGEGSFLYGVVRQVTITAKTPATLMVFNSKEFKAFVGSFQSL
                     SQMLRYTAQRGPQEEVLGESRWSTELLSSPVESVMKKPAPCLPSSASINDAIIAFGKC
                     AHTLLCIMDGERRLLSIVTKTDVYRAMTHCQDFREPIHTIAKREVAAVHEGQAVREAL
                     GVLYMKDVKQAPVLDDQSRPVGILSHMDVAEARIRLQLEHADGKAPVSERTAIPRQQQ
                     "
     gene            2355..2738
                     /locus_tag="VLA60_15940"
     CDS             2355..2738
                     /locus_tag="VLA60_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HSF10902.1"
                     /translation="MKSYTAQTYALMRIVTGLLFLWHGTQKLFGVPKPPGMELPGYVI
                     NIAGPIELVGGVLVMIGLFTRWAAFLCSGLMAAAYWMVHGSKAFFPIVNGGELAALYC
                     FVFLFISAQGSGIWSADDSMGNSSR"
     gene            2818..>3293
                     /locus_tag="VLA60_15945"
     CDS             2818..>3293
                     /locus_tag="VLA60_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10903.1"
                     /translation="MNRTIHLFLTGLSALMGMFFVSALLGLFLVMPAVSHADSKSVSK
                     GHREVSGVVKTEKGGVITVETPTGNYTLTENAAKRHGHAVPKVGDEVTIVLDENNAVI
                     EAHPKGEEGRHRFYTGKIEYVGKMKKEIKLHTPEGEKVFPLDRLEIKTKPIEEGAMV"
BASE COUNT          792 a          812 c          912 g          777 t
ORIGIN      
        1 ttcgcagatg gtctctacgg acttgtgatg gtgagcacag gatcctaccc ccgcaaaacc
       61 agggaatctc ctagttccct aaaaagagaa atggattaaa agcatgatac acttgggata
      121 atttcggtcg aaataaagga atcaatgatg agaaggcttg atgctcaagc acatgcgtta
      181 attgtatttg atcgcagggg gaaacgacca taatcggtat ccacaagaaa tgggaagtcg
      241 tcattctggg tggaggtttt gccggtctgg cctgcgcccg tcaactcgaa aaaatttggg
      301 ggacagacac ggccaaacgc gtccttctgg tcagcgctga aaattatttt gtgtatcaac
      361 cgtttctttc cgaggtgatc ggcgcgggaa tcgagcctcg ccacgtcatg aaccctatcc
      421 ggttggcctt gcgccattgc atggtccaac gggccgaagt ggcaaaactt gaccttcaac
      481 accggcgtgt ccatttcagc gcggatgagg gagtgaggct tgaaccggtc gaagccgatc
      541 atctgatcct cgcgttgggc agcacgacca atctgcgggc ggtgcccggc atgatggagc
      601 acggattatt cttaaagacg ctggccgatg ccctgggtat acgggaacat atcatcaaac
      661 ggctcgaaga ggcggacctc gaatcagatt ctgatctcag acgagctctc ctgcacttcg
      721 tggtcgtggg cggaggatac agcggagtcg aaacggccgg tgaaattctc gacctgctga
      781 atgacgcaag gcggttttac ccctcgtttg atccatccga gttgcgcgtc accctcgtcc
      841 attccggacc gtatttgctc ccggaactgg gagaggattt ggggagtttc gcacagcagt
      901 ctctcgaatc gcgaggcatg acgatcatgt tgaaccggcg ggcctcctcg gtgagtgctt
      961 cctatattcg cctgagcgat ggaacccggc tcgattctca taatccgatt tgtacgattg
     1021 gcaacgcccc gaatccggta ctcgctgatc tcgccgcgga gtatgagaaa ggatggctgg
     1081 tcactgacga atatttacgc ctcaaaggct atacccatgt ttggggcatc ggagatggcg
     1141 cgtccaaccc cgacggatac ggacatcagg ccccgccgac cgctcaattc gccatacaac
     1201 ttggccggca tgcgtcggaa aacatcgctt cctcaatttc agggcacagc ctccgtccgt
     1261 tccgatataa aatgatgggg cagatggcga ccatcggcca ccataagggc gtctgctcga
     1321 ttttcgggtt ccggttcagc ggattttttg catggtggtt gacccggacc attcatttgc
     1381 tgaaattgcc gggactcgac cgcaaactgc gggtcatggt cgattggacc tttgaacttt
     1441 ttttcccgcc ggatctcaac tacctggatc tgaaaaaaac tcaaaaaatc gcgcgcatgc
     1501 atgtggagcc cggagatgtg atttttcgtc agggggaaca aggctccgcc ttctatgttg
     1561 ttgaagaagg gtcactggaa gccgtcaggc ataataagga gggtgatgtg ttgtggcggg
     1621 acgagttgtt tccgggagat cattttggag agggatcgtt tctctatggt gtcgtccggc
     1681 aggtgaccat caccgcgaaa acgcccgcga ccctcatggt gtttaattcg aaggagttta
     1741 aggcgtttgt gggaagcttc caaagtctca gtcagatgct ccgctacacc gctcaacggg
     1801 gacctcagga ggaagtgttg ggagagtcac gatggtcgac agaattgctc tcgtctccgg
     1861 tcgaatccgt gatgaaaaaa cccgcgccgt gtcttccctc ctccgcctct atcaatgatg
     1921 caatcatcgc atttggaaaa tgtgcgcata ccctgctctg catcatggac ggtgaaagaa
     1981 ggcttctgag catcgtgacc aaaacggatg tctatcgtgc aatgacgcat tgccaggatt
     2041 ttcgcgaacc catacacacg attgcgaaac gcgaggtggc cgcggttcat gaggggcagg
     2101 ccgtccgcga agccttaggg gtgctctaca tgaaagatgt aaaacaggcc ccggtgctgg
     2161 atgatcaatc caggccggtc gggatactta gccatatgga tgtggctgaa gccagaatcc
     2221 gtctccaact ggaacacgca gatggcaaag ctcctgtttc cgagcgaacc gccataccac
     2281 gtcagcaaca atgactatgt ctccaggatg attcgctggt ttttccacat tcacaacgga
     2341 ggacggcaat gtttatgaaa tcctacacgg cccaaacgta tgccctgatg agaatcgtca
     2401 cgggactgct gtttttatgg cacggcacgc aaaaattgtt tggcgttccc aagccccctg
     2461 gcatggaact tcccgggtat gtcataaata ttgccgggcc tatcgaattg gtcggtgggg
     2521 ttcttgtcat gatcggatta tttacaagat gggccgcttt tttatgcagc ggactcatgg
     2581 cggcggccta ttggatggtc cacggatcca aagcattctt tcctattgtg aatggtggtg
     2641 aacttgccgc attatattgt ttcgtgtttt tatttatttc cgctcagggt tccggcattt
     2701 ggagcgcgga tgattccatg ggaaattctt cacgatgatc aaggaacgtg ctgataggca
     2761 ttatcgtaag ccgtccctca gggacggaaa tattactttt taataaagga gcacaacatg
     2821 aatcgtacca ttcatctctt cctcactggg ttgtcagccc taatgggtat gttcttcgtg
     2881 tccgcactat tgggcctttt tctcgtcatg cctgcagtca gccatgcgga tagcaagagt
     2941 gtcagcaaag ggcatcggga agtcagcggc gtagtcaaaa ctgagaaagg gggagtcatc
     3001 acggtcgaaa cgccgacggg caattatacg ctgacagaaa atgccgccaa gcgacatggc
     3061 catgccgtcc ccaaggtggg agatgaagtg acaattgtcc tggacgaaaa caatgcggta
     3121 atcgaagccc atccgaaagg cgaggaggga cgtcatcgct tctataccgg caaaattgaa
     3181 tatgtaggga agatgaagaa ggaaattaaa ctgcacacac cagaaggtga aaaggtgttt
     3241 cctctggacc ggctggagat caagacgaaa cccattgaag aaggtgccat ggt
//
LOCUS       DATIAL010000518         5843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1490858, whole genome shotgun sequence.
ACCESSION   DATIAL010000518 DATIAL010000000
VERSION     DATIAL010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490858"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VLA60_15950"
     CDS             complement(<1..280)
                     /locus_tag="VLA60_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HSF10904.1"
                     /translation="MNYVALKMLFGDRPKYLMLLAGLTFSTMLIVQQGSIFWGLMTWS
                     QSGITNVNAPVWVTDSNINQVEEIKPLADTTVNVVRSVSGVQWAVPLYK"
     gene            complement(277..951)
                     /locus_tag="VLA60_15955"
     CDS             complement(277..951)
                     /locus_tag="VLA60_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HSF10905.1"
                     /translation="MPLSPKRISGPVRKAKIIQSAASLFAKKGFSGTKTREIAQRAGV
                     SEALIFKHFPSKEDLYAAILAEESPVPGLLSQIRSLAEQQKDVEVFSVIARTVVGGAP
                     DSDLMRLILFSALENHEMSDMFFHNHIRVFYDFLAGYIQQRINDGAFQPIQPLIAARA
                     FMGMLIYHRLLTVLFQAQLPQTAADISQTFVNLMMNGLKLPPPKGAVRRSGQTHKPRS
                     RTARKV"
     gene            1355..3796
                     /gene="lon"
                     /locus_tag="VLA60_15960"
     CDS             1355..3796
                     /gene="lon"
                     /locus_tag="VLA60_15960"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HSF10906.1"
                     /translation="MDTPPTAENTLIVSLPLVPVKRTVLFPDTLVPFTIGRPRSIAAV
                     EAAMNSEDKALVFATQRDSEKEEPEFDDLYRIGTKAVIKQMGKTPDGHLQVLAQGIER
                     VVLLKKEQDEPHMIVRSRQLALALESDLELEALHRELVELIGQLPELLTTQGVVELVA
                     VLRAEKNPLSVAYRLVSLLNLNVERLQGLLEQSDAKEVLRQVYGALAHELHILKIRHE
                     IASQAQAEIGKSQREYFLRQQLKEIQQELGDLETQPEDGEIGEIKARIEKADLPDIVK
                     TEATRELKRLVKLPPASPDHQVIRSYLELVLELPWNTLTEDHLDLTHVRAILDEDHYG
                     IQDVKERILEHLAVLKLNPSAKAPILCLVGPPGVGKTSLGQSIARALERKFERLSLGG
                     LHDEAELRGHRRTYVGAMPGRLIQALRRAGAKNPILMLDEVDKVGQDFRGDPASALLE
                     ILDPEQNHTFRDNYLDLPFDLSKVMFITTANSLDTISRPLLDRMEIIRLGGYSHQEKL
                     EIANRYLWPRRLKEAGLESKPLTLEAEVIPHIIKRYTREAGVRQLEQMLGRLTRKVAL
                     KLAEHSPDQPEEPLTITQMDLPEWLGIERFMPEEARKTLPLGVATGMAWTETGGDVLY
                     VESALLPGGSDLTITGHIGDVMQESAQAARSYLWSRAESLGLDIAVFKQSGMHIHVPE
                     GAIPKDGPSAGVTMASALASLLLKIPVRKDTAMTGEIGLTGLVLPVGGIKEKILAAHR
                     VGLRRIVLPKANEKDLKDVPDTVRSELSVVFVETIEEALHAVLVNTPDIREREMNPPH
                     TQPAGSEQEPVMGSH"
     gene            complement(3862..4317)
                     /locus_tag="VLA60_15965"
     CDS             complement(3862..4317)
                     /locus_tag="VLA60_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10907.1"
                     /translation="MQKSIVKIFLIIINVFVAVGCADLSHQIPQGEENGPDPSHPPRQ
                     LQDLAGIWEYADKTGSNTITLNEEGKGHYEWEDGWFETHELKDGVWKGKWMQAGNDRE
                     GGFELKWLDGSPVAQGRWWYTRIGQDHNPLEPGGTFTMQRISSFLTGGK"
     gene            4643..5839
                     /locus_tag="VLA60_15970"
     CDS             4643..5839
                     /locus_tag="VLA60_15970"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HSF10908.1"
                     /translation="MTTHRTDSSAESSSDVATWSPDQRDQTEALHGIPPSGQDNEPDD
                     SDLFPIHHPIFWGRGRTLFGIFIVNTFFTLLTLGLYSFWGRVRIRQFLSSQTSFAHTR
                     FSYHGTPQELLKGWSKAFLVFGLPYAFLSLVPLIWDQIPTWIPNALAGTMALCFIPVA
                     VVGSHRYRLSRTSLGTIRFSFRGHVKEYTKIWVLGSVLTLLTAGIYYPYFENARRKFL
                     VAHTYFGNRRFTYSGTGSGLLRIYVTSLAFIMIILFTLIGISAGPDGLSLLAQWPSDG
                     WRDLFLNSLFITGLLTLLLPWFYLQVAKQRYQWNHSDFGDAHFHFSASTWNLMELRIT
                     NFLMLILTFGLAWPWVQVRNLQFLYYHLSLQGPLDFQRIEQEALDASPTGEELAGYFD
                     AGFDLG"
BASE COUNT         1420 a         1568 c         1436 g         1419 t
ORIGIN      
        1 ccttatagag aggaaccgcc cactggacac cggaaacact tcgaaccacg ttcaccgtcg
       61 tatcggccag cggcttaatc tcttcgactt gattgatatt ggaatcggta acccacacag
      121 gggcattcac attggtgatg cccgattgcg accaggtcat caacccccag aaaatcgatc
      181 cctgctgaac gatgagcatc gtggaaaacg tgagccccgc cagcaacatg aggtatttgg
      241 ggcgatcgcc aaacaacatt ttcagtgcaa cgtaattcat accttccgtg ccgttcgcga
      301 ccggggttta tgtgtctgcc ctgatctgcg taccgctccc ttcggtggag ggagtttaag
      361 tccgttcatc atgaggttca caaaggtctg cgaaatgtct gcggcagttt gaggaagttg
      421 agcctgaaat aagaccgtca ataaccgatg ataaatcagc atgcccataa aggcgcgtgc
      481 agcgatgagg ggctgaatcg gctgaaaggc tccatcattt atgcgctgtt gaatgtagcc
      541 ggccaaaaaa tcgtaaaaca cccggatgtg attgtggaaa aacatatccg acatctcatg
      601 gttttccagt gcgctaaaca aaatcagcct catcaaatcg gaatccggag cccctccgac
      661 caccgtccgg gcaatcacgg aaaacacctc aacatccttt tgttgctccg cgagacttct
      721 tatttgggaa agtaatcccg gcacgggcga ttcctccgca agaatggccg catacaagtc
      781 ctctttactg ggaaaatgtt taaaaataag ggcttcgctg actcctgccc tctgagcaat
      841 ctcccgggtc ttggtcccgc taaacccttt tttggcaaat aaggaggccg ccgattgaat
      901 gattttggcc ttccggaccg gaccggatat tcgtttcggt gataatggca tcttcacctt
      961 ctaatataat gatatagtga gtaatcactt accatgctaa gatctcaccg gaaaactgtc
     1021 aaggaatccg gatcatttgc aattttgtcg ggaacggtgg aaaattcgta aaacaactta
     1081 tccatcccat cctgttttca tcacgggcat cattactccg gatgatgttc agatggacac
     1141 cagggagatt ccacctcgga ccatgggcac gagagtgcat caccaacaga ggtgcaggtt
     1201 ttcgatttca ctcatgaatg gctttttgac tctaccccac atggcacaac ccgtaggtcg
     1261 tgccaatcaa tttgactcaa cggaaatgat gcgtatcttg ttggggagat caattatcat
     1321 gtaggtatac acctaaagga cggtgacgat acaaatggat actcccccaa ctgccgagaa
     1381 tacactcatt gtttccctcc cgctcgtccc tgttaaacga acggtgttgt ttcccgatac
     1441 tcttgttccc ttcacgatcg gccgaccccg ctcgatcgca gcggtggaag cggcgatgaa
     1501 ctcggaagac aaagccttgg tgttcgccac ccagcgtgac tcagaaaagg aggaacccga
     1561 gttcgatgac ctgtatcgca tcggcacgaa agccgtcatc aaacaaatgg gcaaaacccc
     1621 ggacggccat cttcaggttc tcgcacaggg gatcgaacgc gtggtcttac tgaagaagga
     1681 acaagacgaa ccccatatga tcgttcggtc ccggcaactc gctcttgcgc ttgaatccga
     1741 ccttgaactg gaagccttgc atcgtgaact ggttgaactc attggccagt taccggaatt
     1801 attgacgacc cagggcgtgg tggaattggt ggcggtgctt cgagccgaaa aaaatcccct
     1861 ttcagtggcg tatcggctcg tgtccctcct caacctcaat gtggaacgat tgcaagggct
     1921 tctggagcaa tccgatgcca aggaagtcct tcgccaggtg tatggcgcgt tggcccatga
     1981 actccatatc ctcaaaatcc gccatgaaat cgcgagccag gcccaagcag aaattggaaa
     2041 atcccaacgg gaatatttcc tgcgccaaca gttaaaagaa attcaacaag aactgggaga
     2101 cctggagacc caacccgaag atggcgagat aggagaaata aaagcccgta tcgagaaggc
     2161 cgaccttccc gacatcgtga aaactgaagc caccagagaa ctgaagcgtc tggtcaagct
     2221 gcctcctgcc tccccggatc atcaagtcat tcggagttat ctcgagttgg ttctggaatt
     2281 accgtggaac accctcacag aagaccacct ggatctgact catgttcggg cgatattaga
     2341 tgaagatcat tacggcattc aggatgtcaa agaacgcatc cttgagcacc tggctgtgtt
     2401 gaagttgaat ccttctgcca aggctcccat cctgtgtttg gtaggacctc cgggggtggg
     2461 aaaaaccagc ttggggcaat ccattgcccg cgccctggaa agaaagtttg aacgcctgag
     2521 tttagggggt ctccatgacg aagccgaatt gcgtgggcat cgccgcacat atgttggcgc
     2581 gatgccaggc cgtctgattc aggctcttcg acgtgccggg gccaaaaatc cgatcctgat
     2641 gctggacgag gtcgataagg tcggacagga tttccgggga gatcccgcgt cagccttgtt
     2701 agaaattctt gatccggaac agaatcatac cttccgcgac aattacttag atctgccctt
     2761 tgacctctct aaagtgatgt tcatcaccac ggccaattcc ctggacacca tctcccgtcc
     2821 tctcttggat cgcatggaga tcatacgtct gggaggctac agtcatcagg aaaaacttga
     2881 aattgccaat cgctatttgt ggcctcgtcg cttaaaggaa gccggcttgg agtccaaacc
     2941 cctgacctta gaggcagagg tgattcccca tatcatcaaa cgatataccc gggaagccgg
     3001 cgttcgccaa ttggaacaga tgttgggacg gttaactcgc aaagtggcgc tgaagctggc
     3061 agaacattcg ccggaccagc cggaagaacc tctcacgatc acccaaatgg accttccgga
     3121 atggttgggc atcgaacgat ttatgcccga ggaagccaga aagacattgc ctctgggcgt
     3181 agcgacggga atggcctgga cagaaaccgg cggggatgta ctctatgtag aaagtgccct
     3241 tctcccggga gggagcgacc tcaccattac cggccacatt ggcgatgtca tgcaggaatc
     3301 cgcccaagcg gctcgaagct acctctggtc acgtgccgaa tcgctgggcc tggatatcgc
     3361 ggtgtttaag cagagtggaa tgcatattca tgtgccggag ggcgccattc ccaaagatgg
     3421 gccatccgcc ggtgtgacca tggccagtgc tctggcttct ctcttactga aaatcccggt
     3481 taggaaggat acggcgatga ccggagaaat tgggttaacc ggattggtcc tgcctgtggg
     3541 cgggattaaa gaaaagattc tggcggcaca cagggtcggg ttgcgccgca ttgtccttcc
     3601 caaagccaac gaaaaagatc tgaaagatgt gccggacact gtgcgatctg aattgtccgt
     3661 tgttttcgta gaaacgattg aagaggcctt acatgctgtc ctggtcaata cgccggacat
     3721 tcgcgagaga gaaatgaacc ctcctcacac ccagcctgcc ggaagtgagc aggaacccgt
     3781 catgggcagt cactaagctt tcagggattg tcttctctcc agccttgatt cttctctcgt
     3841 tatggaaata ccgcgatgag gctacttccc acctgtaagg aacgaggata tccgttgcat
     3901 ggtaaaggta cctccaggct caagaggatt gtgatcctgg ccaatgcggg tgtaccacca
     3961 acgtccctgg gctaccggcg aaccatctaa ccacttcagc tcaaaccctc cttcccgatc
     4021 gttcccggcc tgcatccatt tgcccttcca taccccatcc ttcagttcat gggtttcaaa
     4081 ccagccatcc tcccactcat aatgcccttt cccctcttca tttaaggtga tggtattgga
     4141 cccggtttta tctgcatatt cccagatccc ggcaagatct tgaagttgtc gaggcggatg
     4201 ggaggggtca ggtccattct cttccccttg aggaatctga tgtgagagat cggcacaacc
     4261 caccgcaaca aaaacattta tgatgatcag gaatattttg actatactct tctgcataac
     4321 tcttccctcg ctcccccggg ataattgagt tgaaaaaata accttaggat tgtatcagat
     4381 aggaccgata gacccagata ggtgaaaccc tgcatcccga aaggccacca ctgatgatcc
     4441 ccatcaagac cgaggcgcct gctcgatgga attgacgccc gcgttgcttt gcacaaaatg
     4501 cgggtcggtc agcaatggta gagagacctg ttcgcaatgt ggagccgcgc ttcattctcc
     4561 acaacaggaa tccgtgaacc actccaatga accactactc agaacagatc gttccttcat
     4621 ttcatcagaa tctcaggagc cgttgaccac tcatagaaca gactcctcgg ctgaaagtag
     4681 ttctgatgtg gccacttgga gccctgatca gagagaccag accgaggccc ttcacggaat
     4741 acccccttcc ggccaggaca acgagccgga cgattccgat ttgtttccca tccaccatcc
     4801 cattttttgg ggccgggggc gaacgctctt tggcattttc atcgtcaata cctttttcac
     4861 cttactgaca cttggcttgt attctttttg ggggagagtg cgtattcgcc aatttttgag
     4921 cagtcagacc agttttgccc acactcgctt ttcttatcat ggaactcctc aggaattatt
     4981 gaagggctgg tcaaaagcgt ttttggtttt tggtcttccc tatgcttttc tcagtctcgt
     5041 tccccttata tgggaccaga ttccaacctg gattcccaat gcgcttgcag ggaccatggc
     5101 cttgtgcttc attcctgtcg ccgtggtcgg atcgcatcgt taccggttga gtcgaacctc
     5161 tttgggaacg attcgtttct ccttccgggg gcacgtgaaa gaatatacga aaatatgggt
     5221 cctgggaagc gtactgacct tattgaccgc cgggatctat tacccgtatt tcgaaaatgc
     5281 cagacgaaaa tttctcgtcg ctcacaccta ctttggtaat cgccgattca cctactccgg
     5341 aacgggaagc gggctcttga ggatctatgt cacatccctc gccttcatca tgataatttt
     5401 gttcaccctt atcgggatta gcgccggacc tgacggtctg tccctcctcg cacaatggcc
     5461 atccgatggt tggcgagacc tctttttgaa ttccctattc atcaccggat tgctcacgct
     5521 gctgcttccg tggttttacc tgcaggtagc caagcaacgg taccaatgga atcattcaga
     5581 ttttggagac gcgcattttc atttttctgc ctccacatgg aatctaatgg agttgcggat
     5641 tacgaacttc ctcatgctca ttctcacgtt tggattagcc tggccgtggg tccaggtccg
     5701 gaatctccaa tttctttatt atcatttgag ccttcaaggc ccgttagatt ttcaacgcat
     5761 cgaacaggaa gccctggatg cgtcacccac cggagaagag cttgcgggat actttgacgc
     5821 tggctttgat ttaggctaat ttg
//
LOCUS       DATIAL010000519         3746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_416849, whole genome shotgun sequence.
ACCESSION   DATIAL010000519 DATIAL010000000
VERSION     DATIAL010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416849"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..785)
                     /gene="nth"
                     /locus_tag="VLA60_15975"
     CDS             complement(81..785)
                     /gene="nth"
                     /locus_tag="VLA60_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HSF10909.1"
                     /translation="MRALASPIFLTKASPHPIESPIAAKKRARRILAALEQSIPYPRV
                     ELDSSNPLELLMATILSAQCTDERVNQVTPKVFSRFRTALDFAEADQAELEALIRPTG
                     FYKNKARHLIGCGQALVKQFHGKVPGTMEALTSLPGVGRKTANVILGSYMGQPAIVVD
                     THVKRVANRLGLTKSQDPAKIEKNLQHLFPKEQWTVGTQRLLLHGRYVCLARTPHCSD
                     CVVYADCGWTGKRKPI"
     gene            complement(782..2431)
                     /gene="hflX"
                     /locus_tag="VLA60_15980"
     CDS             complement(782..2431)
                     /gene="hflX"
                     /locus_tag="VLA60_15980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HSF10910.1"
                     /translation="MERLYRRRIPPEKVVTSEIARECAELTYDTRRQIGLLVNRQGQI
                     ESVLIGDHHELVIPHLSRTRSGLRLLRGVRLVHTHLNNQPLTQDDLTDLALLRLDLIM
                     ALGVGKDGTLEDVYMAHILSHPIHGRTTLELPPCAFPVFQLDCQQFIQSLEQEIVRTS
                     PGLREGVREGRALLVSVSAGHAASEQGRLEELQELVRAQDLEVLGCITQRLGAIHPKY
                     VVGSGKMKELAIRALQSGADTLIFDQDLTPAQVRGISEITDLRVLDRSQVILDIFAQR
                     AHSSIGKIQVELAQLRYLLPRLAQSSTALSRLAGGIGTRGPGETKLETDRRRARERIQ
                     RLERDLESMANGRQEQRKMRVKRGLPIISIIGYTNVGKSTLFNALTDSHESVKNRVFE
                     TLDTVNRRWHLQGLGNVILTDTVGFIRDLPKDLVAAFQTTLQELREADILLHVIDAHV
                     PDPGLHITAVENILNELGLENIPCLRFFNKADLIEEEDIQLLCQRFGGIGGSALQGAS
                     LQKIKRELISLFRGLHIEGVWRGEAQERPPHHSMTSAGSLP"
     gene            complement(2502..3563)
                     /gene="tsaD"
                     /locus_tag="VLA60_15985"
     CDS             complement(2502..3563)
                     /gene="tsaD"
                     /locus_tag="VLA60_15985"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HSF10911.1"
                     /translation="MLPVVLGIETSCDETAVALLDDDGHILANVLDSQIDVHARYGGV
                     VPELASRRHMECIEHLTKEALDLAKVSLSQLTGIAVTNRPGLVGALLIGVNFAKALAY
                     ATNLPCVTVNHLEGHLASAWLANPNLSTPAMVLIASGGHTHLALVPHQGEYRFIGWTM
                     DDAAGEAFDKGAKMLGLSYPGGPAIDKLAEKGNAQFVSFPRPNIHSQNFNFSFSGLKT
                     ALRYFLRDEQQKGNLSLPIADVAASYQEAIVEVLVEKLCRAAREYNVKAISVVGGVAA
                     NSRLRVKLEERAHTLGLEVALPPRALCTDNAAMIAAAGLKKLRCQEKTAWDMDAISTL
                     QFKHDGSDMVGIRSKLNIL"
BASE COUNT          964 a          995 c          849 g          938 t
ORIGIN      
        1 atgattcacc gaacgaattt caacaccgat cattgtccca tggcagagcg attctcgttt
       61 tccaaaaccg gtcatacttt tcatatcggc tttcgttttc ccgtccatcc acagtctgcg
      121 tacactacgc aatcagaaca atggggtgtt ctggccaggc acacataccg gccatgaagg
      181 agcaggcgtt gggtccctac agtccactgc tctttgggaa agaggtgctg aagattcttc
      241 tcgattttgg ccggatcttg gcttttcgtc aacccgagtc gattggccac gcgcttcaca
      301 tgggtgtcca ccacaatggc gggctgtccc atatagctcc ctaaaattac attcgcagtt
      361 tttcgcccga ctccaggcaa ggaagtcaac gcttccatcg taccgggtac ctttccatga
      421 aactgtttga ccagagcttg tccacatccg ataagatgac gcgccttatt tttataaaat
      481 cccgtgggtc gaatgagggc ttccaattct gcttgatcgg cctctgcaaa atccaaggcg
      541 gtgcgaaagc gggaaaaaac cttaggcgtc acctgattaa ctctctcgtc tgtgcattgt
      601 gccgaaagaa tcgtcgccat taataactcc agcgggttcg aggaatccaa ttccaccctg
      661 ggatacggaa tcgattgttc caaggcagcc aaaatccgtc ttgcacgttt ttttgccgct
      721 atcggagact cgatcggatg cggtgaagct tttgtgagaa atattggtga ggcaagagcc
      781 ctcatggaag agaacccgct gaagtcatcg aatgatgagg aggacgctct tgggcttctc
      841 ccctccagac accctctatg tggagaccac gaaacaagct tatgagctct cgtttaatct
      901 tttgaagaga cgctccttgc aaggcagatc cccctattcc tccaaatcgt tgacataaca
      961 attgaatatc ttcttcctca atcaaatccg ccttattgaa aaaccgcaaa catggaatgt
     1021 tttcaagccc caattcattt agaatatttt ccactgccgt aatatgcagc cctggatccg
     1081 gcacatgggc atcaatgacg tgaagtagga tatctgcctc tcgtaattcc tgtaacgtgg
     1141 tctggaaggc cgccacgaga tcttttggca aatctctgat aaatcccaca gtatccgtca
     1201 aaatgacatt gcctaatcct tgaagatgcc atcgccgatt cacggtatca agagtctcaa
     1261 acacgcggtt cttgacagat tcatgactat cggtaagcgc attaaataat gtcgacttac
     1321 ctacattggt atatccaatg atggaaataa tgggaagacc ccgtttcacg cgcatttttc
     1381 gttgttcctg acgaccattc gccatagatt caagatcgcg ctctaaccgc tgaatgcgtt
     1441 cacgggctcg acggcgatca gtctccagtt tggtttcccc cggccctcgg gttcctattc
     1501 cccccgctaa acgtgaaagc gcagtgctcg attgtgccag acgaggaagc agataacgga
     1561 gttgcgccag ttcgacctga atttttccga tagaggagtg cgccctttgc gcgaagatat
     1621 ccagaatcac ctgagatcga tccaagaccc gcaaatctgt aatttcggaa ataccgcgta
     1681 cctgggcggg ggttaaatcc tgatcaaaaa tcaaggtatc ggcacctgac tggagtgcac
     1741 gaatggccag ttccttcatt ttgccagaac caaccacata tttaggatga atggccccca
     1801 gccgctgggt gatacaccct aacacttcta aatcctgcgc acgcaccagt tcttgcagtt
     1861 cctcaagacg accttgttca gaagccgcgt gcccggcgct gacactgacc aggagagctc
     1921 tcccctcccg aactccttct cgtaagcccg gacttgtgcg gacaatctcc tgctccaaag
     1981 attgaataaa ttgctggcaa tccaactgga atacaggaaa tgcacaaggg ggcaactcca
     2041 gggtagttcg accatgaatg ggatgtgaaa gaatatgcgc catatagaca tcctcaagag
     2101 tgccatcttt tcccacaccg agagccataa tcaaatccaa ccgaaggaga gccaggtcgg
     2161 tcagatcatc ctgggtcagc ggttgattat taaggtgtgt atggaccaaa cgaaccccgc
     2221 gtaacaatcg aaggccggag cgggtcctcg acaaatgtgg tatgactaac tcatgatggt
     2281 caccaatcaa taccgattca atttgtcctt gtcggttgac caagagcccg atttgacgcc
     2341 tcgtatcata ggtgagttcg gcacattccc gggctatctc agacgtcaca actttctctg
     2401 gaggaatccg tcgtcggtac agacgctcca tacggctaat gtggttagcc ttcaacccat
     2461 gaagattacc caggacggct ggaataagcc ttctccttac tttataaaat gtttaatttg
     2521 gaccgtatcc cgaccatgtc ggagccatca tgtttaaatt gcagcgtaga gattgcatcc
     2581 atgtcccatg ccgttttttc ttggcacctg agttttttca atcctgcggc agcaatcatg
     2641 gctgcgttgt ccgtgcataa ggcacgcgga ggaagagcca cctccagtcc taaggtgtga
     2701 gcccgttcct caagtttcac gcgaagtcgg gaattggcgg cgactcctcc caccacggaa
     2761 atggctttaa cattatattc tcgcgcagcc cgacagagtt tttccaccaa aacctccaca
     2821 atggcttcct gatagctggc agccacatcc gcgataggaa gggataaatt ccctttctgc
     2881 tgttcgtccc gcaaaaaata acggagtgct gtcttcagtc cgctgaagct aaaattaaaa
     2941 ttttggctat gaatatttgg gcgaggaaag gagacaaatt gtgcattgcc cttttcagca
     3001 agcttatcaa tggcggggcc accaggatag gaaaggccta gcattttggc acctttatcg
     3061 aaggcttctc ctgctgcatc atccatggtc catccaataa accggtattc tccctgatgt
     3121 ggaaccaatg ccaggtgcgt atgcccaccg gatgcaatta agaccatggc aggagttgac
     3181 aaattcggat tagccagcca cgctgatgcc aaatgcccct ccagatgatt caccgtcaca
     3241 caaggtaaat tagtggcata agccaaggct ttggcaaaat ttactccgat caataaggcc
     3301 cccaccaagc ctggacgatt ggtgaccgca attcctgtca attgagataa agaaactttg
     3361 gcaagatcta aggcctcttt ggtcaggtgt tcaatgcatt ccatgtgacg gcgagaggcc
     3421 aattcaggga cgaccccccc atatcgggca tgcacgtcaa tctgtgaatc caatacattg
     3481 gcaaggatgt ggccatcgtc gtccaatagg gccacggctg tttcatcaca cgaagtttca
     3541 atgcctaaaa ccactggcag catcctttat gacgcctgtc attcatatgg caaaaaacaa
     3601 caaaaccctc aggaccggtg gtcctgaggg tttcatcttc gatctgaact acactatttc
     3661 caatcacaca ctcgctaagg cctcttcttc aatgaaattt ggagtgtgat cggttaacac
     3721 aaccttgatc tttttcgcat ctttaa
//
LOCUS       DATIAL010000520         4235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_630369, whole genome shotgun sequence.
ACCESSION   DATIAL010000520 DATIAL010000000
VERSION     DATIAL010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630369"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="amrB"
                     /locus_tag="VLA60_15990"
     CDS             complement(<1..862)
                     /gene="amrB"
                     /locus_tag="VLA60_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HSF10912.1"
                     /translation="MSTETVEKDSKNYPILRNLQYSPLKQGEEQYIVLWDPSGLSSEK
                     LVLPLNFFYLFQFFDGEHSLEQVGAEYLKKYGEFMMPDRLTKLVSDLDEKLFLEGERL
                     KQVQAQAFEAYRKLDSRPMAFAGQQYESDPAKLRAQIDGFYSSKEGPEKGKAECAGQT
                     IKGLVAPNFELKGAGPIYAWGYQELREGQVPDVLVLIGTCHAGLEGGIAFTDKDFETP
                     FGTVPVNRAIIDLIRKETGETFFVEDIAHLREHSLELQLPFLKHALGEGREISIVPIL
                     VDFPPETLTGG"
     gene            1213..1413
                     /locus_tag="VLA60_15995"
     CDS             1213..1413
                     /locus_tag="VLA60_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10913.1"
                     /translation="MGKAESIEQQIRTLSPEELAKFRAWYASFDAQAWDAQFTADVEA
                     GKLDSIAERNIPAHEAGQSTKF"
     gene            complement(1930..3588)
                     /locus_tag="VLA60_16000"
     CDS             complement(1930..3588)
                     /locus_tag="VLA60_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HSF10914.1"
                     /translation="MNDSYTIGTLLLDRLYKLGLHHIFGIPGDYVLTLFKLIEESPIR
                     HIGTTREDCAGFAADAYARIHGIGGACVTYCVGGLNMVNAVACAYAERSPVVLISGSP
                     GLKERVNNPFLHHMVRDFSTQRDVFEKITVASVVLDDPHTAEREIDRALKALMQFKRP
                     IYLEIPRDLVMTPVQVASTTPPTVTACQSDPAALKEAVAEVRGILSGSERPVVLAGAE
                     IHRFGLQDQLTELVEHMNVPIATTLLGKSVLREDHPLNIGVYGGLVGREEILEFVENA
                     DCLLTLGTLLTDVEDVKAHATLLAAGRTVHATADSIAIKHHKYEDVRFEDFIRALVAS
                     PLPSFPARALPPRDSVRFDPPGPEAAVTLRNVFGYLDGLLNEKTVVIADVGESLFAAA
                     DLRVRKSADFLSPAYYTSMGFSVPAALGAGFADPGLRPLVLVGDGAFQMTGTELSTCI
                     RYGQAPIVVVLNNRGYGTEREILEGPFNDIHEWQYEKICDVLGGGVGHRVGTFGDLVQ
                     ALSLAVDDHKQVHVLNVLLDPRDRSTAMKRVAQRLAKRMAGQKT"
BASE COUNT         1142 a         1130 c          985 g          978 t
ORIGIN      
        1 ccccacctgt caatgtctca ggaggaaaat ccacgagaat cggtacaatg gaaatctccc
       61 gcccttcacc cagcgcatgc ttaagaaacg gcaattgcaa ctccagacta tgctctcgaa
      121 gatgggcaat atcttccaca aaaaaggttt caccggtttc tttgcggatt aagtcgataa
      181 tcgctcgatt aaccggcacg gtcccaaacg gcgtttcaaa atccttatcc gtaaaggcaa
      241 ttcccccttc gagaccggca tggcaggtgc cgatcaacac caacacatcc gggacctggc
      301 cttcccgtaa ttcctgatag ccccacgcgt aaataggccc ggcacctttc aattcaaaat
      361 tcggggcgac aagcccttta atcgtctgac cggcacattc cgctttccct ttttccggac
      421 cttctttgga ggaataaaaa ccatcgatct gcgcccgcag tttggccgga tcggattcat
      481 attgctgccc ggcgaacgcc atcggtctcg agtcaagctt tcgataggcc tcaaaggctt
      541 gcgcctgcac ctgcttcagt cgctctcctt ccaaaaacaa tttctcgtcc agatcgctca
      601 ccaattttgt gaggcgatcc ggcatcataa attcgccata cttcttgaga tactcggccc
      661 caacctgttc cagggaatgt tctccatcaa aaaactgaaa cagatagaaa aaattcagcg
      721 gcaataccag cttttcagaa gacaggccgg aagggtccca caacacaatg tattgctcct
      781 ccccctgctt tagcggggaa tattgcaaat tccggagaat cgggtagttt ttggaatctt
      841 tttcgacggt ctcggtactc atatggaagg tcctatcctg gctgaaagga aaaatttgtc
      901 tcaaaccaga ccattatacg ggcgcatgaa tcaagcccgc aaccaatcag gacaagggtt
      961 tttgatgaat atcgtgaaag cgaaaaatga aaggggttta cagagaattg tgagatgaga
     1021 gcccccatga gctacgtgca aaggtagcta agggataacc agcgattcca taggtttcat
     1081 ttggtggaaa tattaaaata gcaaggccta gcaaacccga atggcccgaa gaagctacca
     1141 ttatgcgatg atcgatagct gacagcagcc atgtctcttg atatactttt tctatattgt
     1201 gaggtaatta aaatgggtaa ggctgaatcg attgaacaac agataagaac tctttcgccg
     1261 gaggaactgg ccaaatttcg tgcatggtat gcctcatttg atgcccaagc ctgggatgct
     1321 cagtttaccg ctgatgtcga ggcgggaaaa cttgattcca tcgctgagcg aaatattccc
     1381 gcccacgaag ctggacaatc gaccaagttt tgaatcacta cgcctctcct gaattttggt
     1441 tttgctatcg gtctcttcct cctcccattc aggtttttgc ggataaggcc tttgagcagc
     1501 taaagactaa cctacgccat ccctccctgc atttcaaaaa agtcactcat tattggttag
     1561 ctcgagtagg tttacactat cgcgcgattg gcgttgacgt ttcggatggg gtcctgtggt
     1621 ttttgatcgg aattcacgcc gactacgaca aactccttcg ttagccctac aatacttcaa
     1681 ctttgaacaa atatatctcc aatttcctag gagaaggcca gggtgagaga gcattaaccg
     1741 tcgaacgtta aacgaatcgt cagtgcgtct cgtagggaaa gggtttggcg ctgtgatctc
     1801 tagaggcaaa acatggcacc cagcttgaat cccgaaagca gctccgtcca cgaggaaaac
     1861 caggccgaat tctttataaa aaattttcct agtgtggcgc tcatagcaga tcaaagttaa
     1921 aaagggttct caggtctttt gtcccgccat ccgtttggct aatcgctggg caacacgttt
     1981 catggcggtg gatcggtcac gaggatcaag cagcacattc agcacatgga cctgcttatg
     2041 atcatcaacg gcaaggctca aagcttggac taagtctccg aacgtgccca ctcgatgacc
     2101 tacacctccg cccaacacat cacaaatctt ctcgtactgc cattcatgga tgtcattgaa
     2161 gggaccttcg agaatttccc gttccgtccc atagccgcgg ttattcagca ccaccacaat
     2221 gggggcctgc ccatagcgaa tgcaggtgga caattcggtg ccggtcattt ggaatgcgcc
     2281 gtcaccgact aacacaaggg gcctcagtcc gggatcagca aacccagcac cgagagcggc
     2341 cgggacacta aatcccatgg aggtgtaata ggccggagac aggaaatccg cactttttcg
     2401 tacacgcaga tccgcagcgg caaacagcga ctccccgaca tcggcaatca ccaccgtctt
     2461 ctcgttgagc aaaccatcca aatacccgaa cacattgcga agggtcactg ccgcttccgg
     2521 accaggtgga tcaaaacgga cgctatcgcg tggcgggagc gctcgtgcag gaaatgacgg
     2581 caagggggac gccaccaggg ctcggataaa atcttcaaaa cggacgtctt catatttatg
     2641 atgcttgata gcaatggagt ccgccgtcgc atgaacggtc cgacctgccg ccaggagcgt
     2701 ggcatgtgct ttgacgtcct caacatcggt gagcaatgtt cctaaggtca gcagacagtc
     2761 ggcattttcc acgaactcca gaatttcttc ccgccccacc agtccgccat acaccccgat
     2821 attcaatgga tggtcttcgc gaaggacgga cttccccagc aaggtggtgg cgatgggcac
     2881 attcatatgt tcgaccagtt ccgtcaattg gtcctgaagg ccgaatcgat ggatttcggc
     2941 gccggccaga acaaccggac gttcggaacc ggacagaatg ccgcgaactt cggcaacggc
     3001 ttctttgagt gccgccggat cactctggca ggccgtgact gtcggtggtg tggttgaggc
     3061 cacctgcacg ggagtcatca cgaggtctcg tggaatttcc aaatagatgg gccgcttgaa
     3121 ttgcattaat gccttcaagg cccggtcaat ctcccgttcg gcagtatgag ggtcgtccaa
     3181 cacgacggaa gccacggtaa tcttttcaaa gacatcacgc tgggtcgaga aatcacggac
     3241 catgtgatga aggaagggat tattcactcg ttccttcagg ccgggtgagc cggaaatcaa
     3301 gaccaccgga gaccgctcgg catacgcaca ggcaacggca ttgaccatat tcaaaccacc
     3361 cacgcaatag gtcacacatg cgccgccaat gccatgtatg cgcgcatagg catccgccgc
     3421 aaagcccgcg caatcttccc gtgtcgttcc tatgtgccgg ataggcgatt cttcaatgag
     3481 cttaaagagc gtcagcacat aatcgccggg aatgccaaaa atatgatgca gccccagctt
     3541 gtacaaacgg tccagaagaa gcgtgccaat ggtgtaagag tcattcatta tgtatcaaac
     3601 ctcatggtca ttatatcgga ataaagtcca attaaaagaa gccacaacct aataagtaaa
     3661 cttaaacaaa tctcaacgga aagaaaataa agaggatacg ttgaaaaaat tggatttctc
     3721 cattcaggaa ttggacaaac tcggtgatga ctcggagctg ccctaaaaca gattgtcatc
     3781 ctgacccatt cgacaaagct ctggacaaaa ctcatggcga aggatctgtg actaggaaga
     3841 ggagccaggg ctcaacgaaa tccaacattt agaatgaagc ctttccaaca accgttcaca
     3901 attctgtaac catgcctagc ttgttccatt caacttttca gcaatattcc ttgtatcgaa
     3961 cctgtattcc catcggtatt ccttcaaatc tccattgcat acgcggtcat ggtgtgcccc
     4021 cccttgtgcc gcctacccgc ccacagccac ctgttgcgat agctagactc tttcgatcgt
     4081 cagataaaac ccgataccac cacaataatt ccctggagga catcgatgaa ctataaaaac
     4141 tcaatggaga gccaaaataa ggttgctttt attcactcaa agagtaaaga ttcctcaatc
     4201 caagggatta caactttctc ggcatggagg ccgcg
//
LOCUS       DATIAL010000521        17005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_953041, whole genome shotgun sequence.
ACCESSION   DATIAL010000521 DATIAL010000000
VERSION     DATIAL010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953041"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VLA60_16005"
     CDS             <1..379
                     /locus_tag="VLA60_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10915.1"
                     /translation="FAFKWRGLSIQHEYHWKEVKDNSYPEGAPGHKTNMMGSYSQIGY
                     FPHYLIPAVPKPLEVAFRYAFVDPNISAPNDRRQEYTTAINWFFAGHRNKITLDGSWL
                     TLAQPAGQNQHEQRVRLQWDVSF"
     gene            693..1208
                     /locus_tag="VLA60_16010"
     CDS             693..1208
                     /locus_tag="VLA60_16010"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HSF10916.1"
                     /translation="MKTPKSPCYDGHPYPITEAMADDLRAILAASRIAIHLERIGDYA
                     KNTTNRSRHLSQPIATELVDQFQWMGKRIQSMLSQVTESYIHHNAPLANVAWADDAEL
                     DTVYANLFQHLLRTMSKETRNIADGTQLLFIAKGLERAGDHVTDIAEEVYLMVTGVPL
                     QGPRPKISELS"
     gene            complement(1307..2035)
                     /locus_tag="VLA60_16015"
     CDS             complement(1307..2035)
                     /locus_tag="VLA60_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10917.1"
                     /translation="MKRSARLMGLMVVGIFLGGCQTPQFQTLKIFDSPNRVVALQAMQ
                     DAYGGKGYDHPVSFTNEEMIQILLGIRAEKTGLYSSSSSRNSSAHPVFSQSEIQFFAP
                     LIVKGLSQATPEEMVTFFETAEIETDDLEKNFAITTSGGFYVAGGNFHVVVSNFSVKT
                     PLWQDAQRSSYDVNAVRTNSLEQLKPQPGLLVFEPREFLVDSPDGEIGSFFKGKPWQV
                     AIRYREFLSHSNDSQSQKNKREPE"
     gene            2223..2606
                     /locus_tag="VLA60_16020"
     CDS             2223..2606
                     /locus_tag="VLA60_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10918.1"
                     /translation="MVRVEQLMTRELACVEFTQAVSVAANLMRIRKIGSLLVKRGEEL
                     VGIVTESDIVRKVVAFHYPSEYIQVGDIMSSPLVSIDESESIFEAAGIMQAAHTRHLV
                     VGNESHVFGMLSVRDLLAPVAKDEL"
     gene            2611..3138
                     /locus_tag="VLA60_16025"
     CDS             2611..3138
                     /locus_tag="VLA60_16025"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HSF10919.1"
                     /translation="MKATIPSTDPSLFNSSFQQSSAWLAKALEADLTHFWMKGIKAVL
                     SLLIVTILVGLAGGVLRIFLNLEALFTEPLEQVFRQLIVDTLIILAIVEVFKTTVTYF
                     SEGRVKVTFIVDTILVVMLTEIISKWFSEAHLDQWMILGGILVVLAIIRVVAVQWSPT
                     RAENAPEWTPPQKSH"
     gene            complement(3506..4600)
                     /locus_tag="VLA60_16030"
     CDS             complement(3506..4600)
                     /locus_tag="VLA60_16030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HSF10920.1"
                     /translation="MSIEVRQITKSFGPFTALDNVSLEVPTGHLVALLGPSGCGKTTL
                     LRIMAGLESPDSGSILFHGEETTTQTVRERQVGFVFQHYALFRHLTVFENVAFGLRVR
                     PRRLRPSEPEIQKKVHELLQLVQLDWLGDRYPTQLSGGQRQRVALARALAVEPKVLLL
                     DEPFGALDARVRKDLRRWLRRLHDELHITSVFVTHDQEEALEVADRVVVMNQGRVEQI
                     GAPDEVYDRPATPFVYQFLGNVNLFHGRIHQGVAQIGNLELEAPDHQEAVDAQALAFV
                     RPHDLELHRHRTGEQDIETIVRYVNAIGPLVRVELARKEVDELIQVELTQERYRELQV
                     KQGERVFVQLKHLRLFLKEGKQETPRVSLD"
     gene            complement(4597..5505)
                     /gene="cysW"
                     /locus_tag="VLA60_16035"
     CDS             complement(4597..5505)
                     /gene="cysW"
                     /locus_tag="VLA60_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HSF10921.1"
                     /translation="MNDLEPQLPVSSWWEARPTTEPVLIRLALTGLAIGFLGLFLILP
                     LVAVFTQAFEQGVGIYLASLTDPDAVAAIRLTLLTAAIAVPLNVVFGLSASWAIAKFE
                     FRGKQILLTLIDLPFSVSPVISGMVYVLLFGLQGFLGPWLADHDLQIIFAVPGIVLAT
                     LFVTFPFLARELIPLMQAQGSEEEEAALVLGASGWQTFLRVTIPNIKWGLLYGVILCN
                     ARAMGEFGAVSVVSGHIRGLTNTMPLHVEILYNEYNFVAAFAVASLLSLLALVTLAAK
                     SVVEWRSHRDQRSLVELRSASIGEMP"
     gene            complement(5537..6376)
                     /gene="cysT"
                     /locus_tag="VLA60_16040"
     CDS             complement(5537..6376)
                     /gene="cysT"
                     /locus_tag="VLA60_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HSF10922.1"
                     /translation="MIINLFRPRGVLPGFGLTLGFSVLYLSLIVLIPLGALLLKTAEL
                     TLEEFWTIVTAPRVLASYRLSFGASLAAALINLVSGLLVAWVLVRYTFPGRKLLDGLV
                     DLPFALPTAVAGIALTALYAPNGWIGRYLAPYEWKVAYTPLGVTIALTFISLPFVVRT
                     VQPVLQEMESELEEAAASLGAFRWQTFIRVILPTLTPALLTGFALAFARALGEYGSVV
                     FISGNMPMKTEIVPLLIITKLEQYDYSGATAIATVMLLASFVLLLLINVLQWWSGARR
                     TAH"
     gene            complement(6373..7176)
                     /locus_tag="VLA60_16045"
     CDS             complement(6373..7176)
                     /locus_tag="VLA60_16045"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HSF10923.1"
                     /translation="MIGHESIDVRHIGIVAGTAEGAALCYRTICREAEPFLAPHAHPE
                     ITMHTFSLGTYLELIEAGRWVDVADLLSQSSEKLTRAGADFIVCPNNTLHLVFDEVQS
                     PIPWLHIADIVATEAFRQGIRRVGLLGTRSVMNGDVYQSRFERLRIDMVWPDAQDQAR
                     IHDIILTELIPGSVSEASRRECLAIIERLKERGCDAAILGCTELPLLVVSGTPVLPLL
                     DSTRLLAQAAIRHAIRPGVQSEFSRRLASNRQDWGAPCADKNKEGVFTE"
     gene            complement(7173..7493)
                     /locus_tag="VLA60_16050"
     CDS             complement(7173..7493)
                     /locus_tag="VLA60_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10924.1"
                     /translation="MIVVVGGDSVEAIKQRLGTEGAPTIQHWSGRRSRDLQRSLPKDT
                     EAVVVILDRISHSLARKVRAEAGRRGLPISFLGRKWSSRAGDLVGSSTMRHAAKIRRK
                     ERPT"
     gene            complement(7708..8757)
                     /locus_tag="VLA60_16055"
     CDS             complement(7708..8757)
                     /locus_tag="VLA60_16055"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF10925.1"
                     /translation="MMRPTWFKLVPMVVVMMATLLSGQGRSEDVTILNVSYDPTRELY
                     QEFNAAFAKYWKEKTGEDVRIQQSHGGAGKQARAVIDGLEAHVVTLALEYDIDAIAEK
                     SGVIAKNWRSRLPHNSTPYTSTIVFLVRKGNPKNIKDWDDLARPGISVVTPNPKTSGG
                     ARWNYLAAWAFASNKFQKDEKKIQDFMSRLYRNVPVLDSGARGSTTTFVQRGIGDVLL
                     AWENEALLATTKLGQKDFEIVVPSIVVPSSSILAEPPVALVDRVVDKRGTRKVAQAYL
                     EYLYTKEGQEIAAKHFYRPRDPEIAAKYAGQFPEVKLFTVDEIFGGWQKAQKNHFADG
                     GTFDQIYGTVPRPLD"
     gene            complement(8836..10257)
                     /locus_tag="VLA60_16060"
     CDS             complement(8836..10257)
                     /locus_tag="VLA60_16060"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HSF10926.1"
                     /translation="MRMFRELVIGGLFMVLMSGTGGLFAAEPEVTSSREQEPQGSSGH
                     KTLKERLEELEQKQRISDRLRELDQQREEQVAKERPVVGADQNGFFLRSANRAFELRF
                     RGYGQIQLRTFLGDTDDADVHDTFEVRRFRPIFEGTLYRYVTFKVMPDFQQGRVVLFD
                     GYIGFDYWSAVRFRAGKFKPPVGLEMWASGADLLFVERGMTMNLLPNRDIGVMLHGSL
                     LDKRLDYQAGVFNGVRDNTPISGGSNADFDSNNDKDYVVRLMTKPFLTTDVEWLQGLE
                     VGVAGTWGRDNFSPSNFRTPAMGQDGITFFRWSNGVAVNGDRWRLTPQTYYSWGPFGL
                     LGEYVWNIQDLRTSGGTEKRLTNRAWQVAATYVLTGEHASLNGVKPWRTFNPAQGAWG
                     AVELAARYGEVLVDREAFPAFADPSVSARKARVWTVGINWHLGHRVKAQLNYERGIFD
                     GGAPNNKDFDKENAILTQVQIAW"
     gene            complement(10386..10556)
                     /locus_tag="VLA60_16065"
     CDS             complement(10386..10556)
                     /locus_tag="VLA60_16065"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HSF10927.1"
                     /translation="MDLSSHIQEEIFKAIQSLRYGSVEVVVHDSEIVQIERKEKVRLG
                     GNRTKTKTNGSE"
     gene            complement(10650..11111)
                     /locus_tag="VLA60_16070"
     CDS             complement(10650..11111)
                     /locus_tag="VLA60_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HSF10928.1"
                     /translation="MISKKTKYGLRALIYLAQQPKNEPVLISELATQEGIPKKFLEQI
                     LLDLKNHGILQSKKGKGGGYALSKAPSQIMMGQIVRILNGPLAPTLCVSQTAYQKCEE
                     CPDEKTCGVRIMMKEVRDAMASVLDRMSLADVVRDIQMVKQGKDSPLMFSI"
     gene            complement(11945..12367)
                     /gene="tnpA"
                     /locus_tag="VLA60_16075"
     CDS             complement(11945..12367)
                     /gene="tnpA"
                     /locus_tag="VLA60_16075"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HSF10929.1"
                     /translation="MSLKRTRGGVCDLKDHVVWVPKYRRMVLGERVARRLKRIFQEIA
                     ERYGFEIDTQEVLDDHVHIFLSVPPRDSPAQVVQRMKSISARIVFQEFPEVKQQLWGG
                     ELWNDGYFVRSVGDNVTVEVIRRSIKHQHDPKQLELNF"
     gene            12485..12802
                     /locus_tag="VLA60_16080"
     CDS             12485..12802
                     /locus_tag="VLA60_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF10930.1"
                     /translation="MVRVKELMKQDLASVNCGDLVSLAATLMRIRRIGSVFVKQNGHI
                     VGIVTEADIVRKVVSTHRSPDRTTVETIMTAPVIRSRCADIRSGGCDVSIRHPPSGRH
                     PPL"
     gene            complement(13448..13765)
                     /locus_tag="VLA60_16085"
     CDS             complement(13448..13765)
                     /locus_tag="VLA60_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSF10931.1"
                     /translation="MKQYDRDATPGKMVSVFITGIVIGTVATFLLCPQSGRESRGQIR
                     DYVRRKGEDLNDLQDRTVETYQDIVSGTRKGLEDIQAGIKEAVHAGMDEFRKELKDIR
                     SKA"
     gene            complement(14078..15010)
                     /locus_tag="VLA60_16090"
     CDS             complement(14078..15010)
                     /locus_tag="VLA60_16090"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HSF10932.1"
                     /translation="MSFRLSRSESFIQGIKRIVNEQIDLASEELDRRPIDPITVPHQV
                     RKRCKKIRGLIRLAKTGMKNRKTYQQENSRLRDAARMLSDIRDAQVMINTYDHLMAFQ
                     RPEIDRREFGPIRRQLSSRFENLLHERSSLEQSFQEIQASLLKSRQHISDALLNDCRE
                     PVMYEGLKTTYAKAFRAMSDVCTRPTDEGFHDWRKHVKYHWYHFRLVRDVWQPVFSGR
                     SSELHRLEEYLGEDHDLSVFERLLTESREVPRSSDCSYAILIGVIHQRKQMLRTAVRP
                     LGCRLFVESPKDFSKRICAYWKVWTQEPPGAQEV"
     gene            15510..15815
                     /locus_tag="VLA60_16095"
     CDS             15510..15815
                     /locus_tag="VLA60_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10933.1"
                     /translation="MPQRTKKPTRAVHTIEIEIAHIMNQSSCDRCGGLLIKGHCLDVA
                     NPEGELWITTKHCIQCGNVIDPVILKNQKEVLDPPPQAERVPRPRPTFGVALQRRNR"
     gene            complement(15907..16299)
                     /locus_tag="VLA60_16100"
     CDS             complement(15907..16299)
                     /locus_tag="VLA60_16100"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HSF10934.1"
                     /translation="MAHEVVSDGRNEGLRQMLLEKKNEVQKQIDDLILQRREEQERWR
                     GEPAMDDQDMAYRDSSADQLLSLLEIRHRMRVTLDEALTRLREGTYGFCNECGEPVSN
                     ARLKALPFAKACLNCQNVIEGLEKVARS"
     gene            complement(16771..>17005)
                     /locus_tag="VLA60_16105"
     CDS             complement(16771..>17005)
                     /locus_tag="VLA60_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10935.1"
                     /translation="ENGSFSMCTFWYIECLSRAGDVKKAHFLFDKMLGHANHLGLFGE
                     GLGPGLAHLGNFPQALTHLSLISAAYDLNRRMQ"
BASE COUNT         4254 a         4773 c         4026 g         3952 t
ORIGIN      
        1 attcgccttc aaatggcggg ggctgtctat tcaacatgaa taccattgga aagaggtcaa
       61 ggataacagc tatccggaag gtgcgccggg ccataagacc aacatgatgg gaagttactc
      121 acagatcggg tatttccccc actatctcat tccggcggtg ccaaaaccgt tggaagtggc
      181 cttccgctat gcattcgtgg atcccaacat ttccgcaccc aatgaccggc ggcaggaata
      241 caccaccgcc atcaattggt tttttgccgg gcacagaaac aagatcacac ttgatggctc
      301 ctggctgacg ctggcccagc cggcaggtca aaaccaacat gaacagcggg tccgcctgca
      361 atgggacgtc tcgttctaaa ttcaacggga cgcacaggca ctcctcctct cgtcccacaa
      421 acaggcctct gtcggttctc atccgggaga ggcctgttct tccattgtat ctatcgatgg
      481 aatgaaaggc gtgcacgatc tcctacgacc agcctgcaag catccaaaaa atatacaatg
      541 aactcgtcag atacgcccca gtaggattta acctcttgaa gagcgcatta tgagcttccc
      601 atctcatcct aagattgtcg aaaggtgaat cattcaagat tttctatctg ctccaattac
      661 atttcttgca ctgacccata cgaagggttt ccatgaaaac ccccaagtca ccttgttatg
      721 acgggcatcc ctatccgatt accgaggcga tggctgacga cctgcgggcc attctggcgg
      781 cttctcgcat cgcaatccat ttggaacgga tcggggatta cgcgaaaaat accaccaacc
      841 gcagcagaca tttatcacag cccatcgcta ccgaattggt tgatcaattc caatggatgg
      901 ggaaacgcat ccaatccatg ctgtcccagg tcacggagtc ctacatccac cataatgccc
      961 cgttagccaa tgtcgcctgg gccgatgatg ccgaattgga caccgtctac gccaacctgt
     1021 ttcagcatct gcttcgcacg atgagcaagg aaaccaggaa cattgcagac ggaactcaac
     1081 tattatttat cgcgaagggg ttggaacgcg ccggcgatca tgtgaccgac attgccgaag
     1141 aagtatattt gatggtcact ggagttcctc ttcaaggccc ccgtcccaaa atcagtgaac
     1201 tgtcttaatg aacgggttcg cacatccaac cctagcctcc taagccgcag atcagacaaa
     1261 agacttccca caccttttcc gcagagcgca aaaggtgtgg gaaaccttat tcgggttccc
     1321 gcttattctt ctgtgactgg gaatcgttcg aatggcttaa aaactcccga taccggatgg
     1381 cgacttgcca gggtttgcct ttaaaaaaac tcccgatttc cccatcggga gagtccacca
     1441 aaaactcccg cggctcaaag acgagcagac cgggctgagg ttttaattgc tcaagagaat
     1501 tggtgcgaac agcgtttacg tcgtaactcg atctctgggc atcctgccag aggggggttt
     1561 taacggaaaa attgctcacc acaacgtgaa aattcccccc cgctacatag aatcctcccg
     1621 aggtggtgat ggcgaaattc ttttcaaggt catccgtttc aatttcagcc gtttcaaaga
     1681 aggtgaccat ctcttcagga gtcgcttgac tcaacccttt tacaatgagg ggagcaaaga
     1741 attggatttc tgattggctg aataccggat gcgcggagga attcctagag gacgaactac
     1801 tgtatagacc tgttttttcc gcccggatgc ccagcaaaat ttgaatcatt tcctcgttcg
     1861 tgaaagacac tggatgatca tagccttttc caccataggc atcctgcata gcctgtagcg
     1921 ccaccacacg attgggacta tcgaaaattt tcaatgtttg aaactggggt gtctgacacc
     1981 ctcccagaaa gatgcccacc accatcaatc ccatcaatct tgccgatcgt ttcataccga
     2041 tatgcctcca atcaaacata atcactcacc cctgcctttc cgagtcattc taccctagat
     2101 ttccaccgca aaaggagggg agcagacgaa atgttacaca tgcgtcacat acttgcgacg
     2161 caggtttaat acaaagacgg tacattccat catgaacctt catattaaca aaggagaaaa
     2221 acatggtacg agttgaacag ttaatgacca gagaactggc ctgtgtggag ttcacccagg
     2281 cggtatccgt cgccgccaac ctgatgcgaa ttcgcaagat tgggagcctg ttggtgaaac
     2341 gtggggaaga gttagtgggg attgtgacgg aaagcgatat cgtgagaaag gtcgtggcct
     2401 ttcactatcc ttcagagtat atccaagttg gggatatcat gagcagcccc cttgtttcga
     2461 ttgatgaatc ggagtcgatc tttgaggctg ccgggatcat gcaggcggcc cacacgagac
     2521 atttggtggt aggcaatgaa tcccatgtgt tcggtatgct atcggttcgt gatttgttag
     2581 ctcctgttgc aaaggatgaa ttataaaccc atgaaagcca cgataccgtc taccgatcct
     2641 tccctgttca atagctcctt tcaacagtca tctgcgtggc tggctaaagc gttggaagcc
     2701 gacctaactc atttctggat gaaaggcatc aaagccgtcc tgagcttatt aattgtcacc
     2761 atcctcgtag ggctcgctgg aggcgtccta agaatttttt tgaatttaga agcccttttc
     2821 acggaacccc ttgaacaggt ctttcgtcaa ctcatcgtgg acacattgat tattctcgcg
     2881 attgtcgagg tgtttaaaac caccgtcacc tatttttccg agggacgagt caaagtcacg
     2941 ttcattgtcg atacgattct cgtggtcatg cttaccgaaa ttatctcgaa atggttttcg
     3001 gaagcccatc tggatcaatg gatgatcttg ggtggaatat tagtggtatt ggccattatc
     3061 cgggtcgtcg ccgttcaatg gtccccgacg cgagccgaaa atgccccgga atggacgcca
     3121 cctcaaaaat ctcactaatc tcacgcaagc ataacaatat ttcccactcc cttcgggacg
     3181 gaatgaaggt ctaacctgca tcgctatcca atcccgtcac aaccttggag tcatttaaga
     3241 aattaatgag aaagctaacc ggatgtagga aagaggtctg gggatcgtga gaattgccga
     3301 catgattgaa agtcgatcac gaaaaaactg aacgattgct gtcttggaac agttgtgctc
     3361 agaaggatga tggctcgcga caacccacct tactaaacat taagtgtcag aatgtcccca
     3421 tctctggcgc ggaggataag gctgtctctc agcaatcgaa catgtcatga atcagggaac
     3481 tcccgctttg gggcacatgt ctggatcagt caagtgaaac tcgtggtgtc tcctgcttcc
     3541 cttccttcag aaacagccgg agatgcttca gttgcacaaa aacccgttct ccttgcttga
     3601 cctgtaattc gcgataccgc tcctgggtca actcaacctg tatcaattcg tcaacttcct
     3661 tccgtgccaa ctccacccgg accagcgggc caatcgcgtt gacataccgg acaattgttt
     3721 cgatgtcctg ctcaccggtg cggtgacggt gcaattcaag gtcatgcggt ctgacaaacg
     3781 ccaaggcctg cgcatccacg gcctcctgat ggtcaggagc ctccagttcg agattgccga
     3841 tttgcgccac gccttggtga atacggccat gaaacagatt cacattgccc aggaattggt
     3901 agacgaacgg cgtggcggga cggtcgtaga cctcatccgg cgccccaatc tgctcaaccc
     3961 ggccctggtt catcaccacg acccgatccg ccacttccag cgcttcctcc tgatcgtgag
     4021 tcacaaacac actcgtgata tgcagctcat catgcagccg gcgcagccac cgacgcagat
     4081 cctttcgcac tctggcatcc agcgcgccga acggctcatc aagcaggagc actttcggct
     4141 ccaccgccaa ggcccgcgcc aatgccacgc gttgccgttg cccaccggac aattgggtgg
     4201 gataccggtc acccagccag tcgagctgca cgagttgcaa gagttcgtgg acttttttct
     4261 gaatctcggg ttcggaaggc cgcagccggc gcgggcgcac gcgaaggccg aacgcgacgt
     4321 tttcaaagac agtcaaatga cgaaacaggg catagtgttg gaatacgaac ccgacctggc
     4381 gctcccgcac cgtttgcgtc gtggtctctt cgccatgaaa caggatactg ccgctgtccg
     4441 gagattcgag gccggccatg atccggagca acgtggtctt gccgcaaccg gacggcccca
     4501 gcaacgccac gagatgtcca gtgggcactt cgagactgac gttgtccagg gccgtgaacg
     4561 ggccgaatga tttcgtgatt tgacgcactt cgatactcat ggcatttctc caattgaggc
     4621 tgatcgtaat tccaccagac tgcgctggtc ccgatgggat cgccattcca caacgctttt
     4681 ggctgcaagg gtcaccaacg ccagaagcga gagcagcgag gccacggcga acgccgcgac
     4741 aaaattgtat tcgttataga gaatttccac atgcagcggc atggtattgg taagcccgcg
     4801 gatatggccc gacacgaccg acacggcgcc aaattcaccc atcgccctgg cattgcagag
     4861 aatcactcca tacagcaggc cccatttgat gttaggaatt gtcacacgca gaaaggtctg
     4921 ccagccgctt gcgcccaaca ccaacgcggc ttcttcttcc tccgatccct gtgcctgcat
     4981 gagcggaatc aattctctgg ccaaaaatgg aaaggtcacg aagagcgtgg ccagcacaat
     5041 gcccggcacg gcaaagatta tctgaagatc atggtcggcc agccacggac ctagaaatcc
     5101 ctgcaagccg aacaacagga cataaaccat acccgagatg accggcgaga cggagaaggg
     5161 aaggtcgatc agcgtgagga gaatctgttt gccgcgaaac tcgaatttgg cgatggccca
     5221 cgaggccgac agcccgaaca ccacattcaa cggcacggca atggccgctg tcagcaatgt
     5281 cagacggatg gccgccaccg catcgggatc ggtcagcgac gcaagataga ttcccacacc
     5341 ctgttcaaag gcctgcgtaa agacagcaac caggggcaaa atcaaaaaca gccccagaaa
     5401 tccgatggcc agcccggtca atgccaagcg gatgagtacc ggttccgtgg tcggccgggc
     5461 ttcccaccag gaggaaacag gcaattgtgg ttcgaggtcg ttcatgaatt catctctcgt
     5521 tccttgttcg ccaggcttaa tgggcggttc ggcgtgcgcc gctccaccat tgaagcacgt
     5581 tgatcaacag taacaagaca aaggatgcca ggagcatcac cgtggcgatc gccgtggcgc
     5641 ccgaatagtc gtactgttcc agtttcgtga tgatcaggag cggcacgatt tccgttttca
     5701 tcggcatgtt cccggaaatg aacacgacgg agccatactc gcccagggca cgggcaaacg
     5761 cgagtgcgaa cccggtgagg agagcaggcg tcaaggtggg gagaatcacc cggataaagg
     5821 tctgccatcg aaaggccccg agactggccg cggcctcctc cagttccgat tccatctcct
     5881 gaagcacggg ttgcaccgtg cgcacgacaa acggcaggct gataaaggtc aaggcgatgg
     5941 tgacgcccag cggcgtgtac gccaccttcc actcataggg agcgaggtac cggccgatcc
     6001 atccattcgg agcatagagc gcggtcaacg caatgccggc caccgccgtg gggagggcaa
     6061 acgggagatc caccaggcca tccagcaact ttcggccggg aaacgtatag cgcaccagga
     6121 cccacgccac cagcaacccc gacaccagat tgatcagggc cgccgccagc gaagccccga
     6181 agctcagccg gtacgaggca aggacccgcg gggcggtgac gatggtccag aactcctcta
     6241 atgtcagctc tgccgttttc aggagcagcg cacccaaagg aattaacacg atcagactca
     6301 agtacagcac actgaacccc agcgtgagtc cgaagccggg caacaccccg cgcgggcgaa
     6361 agagattgat gatcattccg tgaagactcc ttccttgttc ttgtctgcgc acggcgctcc
     6421 ccaatcttgc cggtttgagg ccaatcgtcg agagaattcc gattgaaccc ctggcctaat
     6481 ggcatgacga atggccgctt gagccagaag tctcgtggaa tccaggagcg gcaagaccgg
     6541 ggtacccgat accaccaaca agggcaactc cgtgcaaccc agaatcgcgg catcacatcc
     6601 ccgttccttc aatcgctcaa tgatcgccag acattcacgc cgtgatgcct cggagacact
     6661 tccaggaatt aattcggtga gaatgatgtc atgaatacga gcctgatcct gcgcatcggg
     6721 ccataccata tcgatccgaa gacgttcgaa cctgctttga tagacatctc cgttcatcac
     6781 gctgcgtgtc ccgagaagac cgacacgacg gattccttgc cgaaaggcct cagtcgcaac
     6841 gatgtcggcg atatgcaacc agggaatcgg cgactgcacc tcgtcgaaca ccagatgcaa
     6901 ggtgttgttg gggcagacaa tgaagtcggc tcccgcacgc gtcagcttct ccgaagattg
     6961 cgacagaagg tcggcaacat cgacccaacg acccgcctcg atcaactcca ggtacgtccc
     7021 gagcgagaac gtatgcatcg tgatctccgg atgcgcatga ggcgcaagga agggttcggc
     7081 ctcccggcaa atcgtgcggt agcacaacgc ggccccttcg gccgttccgg ccacgatgcc
     7141 gatgtgtcgc acatcgatag attcatgacc aatcatgtcg gacgctcctt tctccggatt
     7201 ttggcagcat ggcgcatggt cgatgatccg acaagatcgc ccgcgcgaga cgaccatttt
     7261 cgtcccaaga acgatatcgg aagccctcgc cgtccggctt cggcgcggac tttccgcgcc
     7321 agggaatgac tgattcggtc taaaatgacc acgaccgcct ccgtatcttt aggaagcgaa
     7381 cgttgaagat cacgtgaacg gcgtccactc caatgctgaa tcgttggcgc tccctccgtg
     7441 cccaggcgtt gcttgatcgc ctccaccgaa tcaccaccca ccaccacaat catgaccgat
     7501 cctcctttgt ccatgaacgt ttaccgggag accacacatc cccgacttgc ccatccgggg
     7561 cgaatgctcc cgaggcaagc actcccttta gcctgagcca tactcccgaa cccttagcca
     7621 tccagctctg ctcagtattt ttggattcca cagaggaggg ggagttcaac gtctccattc
     7681 tcctccggga atgttccgct tctttcgtta atccaacggg cgcgggacgg ttccgtaaat
     7741 ttgatcgaac gtgccgccat ccgcgaaatg atttttttgc gctttctgcc atccgccaaa
     7801 gatctcatcg acggtgaata atttgacctc aggaaattgc ccggcatact tggccgctat
     7861 ttccggatca cgcgggcggt aaaagtgctt ggcggcaatc tcctgccctt ccttggtata
     7921 gagatattcc agatacgcct gtgccacctt gcgggttcct cgtttatcca ccacgcgatc
     7981 cactagggcc acggggggtt cggccaggat actgctggag ggcaccacaa tcgaaggcac
     8041 cacaatctcg aaatctttct ggccgagttt tgtcgtggcc agcaacgctt cgttttccca
     8101 tgccaacaag acgtcgccga tgccgcgctg gacgaacgtg gtcgtcgacc cgcgagcccc
     8161 ggaatccaga accggcacgt tccggtacag gcgtgacata aagtcttgaa ttttcttttc
     8221 gtccttctgg aatttattgg aggcaaacgc ccaggctgcc aggtaattcc accgggcccc
     8281 gccggacgtc ttgggattgg gggtcaccac cgagatgccc ggtctcgcca aatcatccca
     8341 atccttaata tttttgggat tcccttttcg caccagaaag acgatggtgg aggtataggg
     8401 cgtactgttg tgcggcaggc gtgaacgcca gtttttggca atcacaccgg atttttccgc
     8461 aatggcatcg atgtcgtact ccaatgccag ggtgactaca tgcgcctcca gtccatcgat
     8521 caccgccctg gcctgtttgc cggccccgcc atgcgactgc tgaatccgca catcctcgcc
     8581 ggttttctcc ttccaatact tggcaaacgc cgcgttaaac tcctggtaca attcccgcgt
     8641 gggatcatac gagacattta agatggtcac gtcttccgag cgtccttggc cggaaagcag
     8701 cgtcgccatc atcacaacaa ccatggggac tagtttgaac catgtcggtc tcatcatgtg
     8761 cgcctctcct tgcgttgagt ctttctaagg taagaaataa atcgtcagta attctcttgc
     8821 atggacgttg atggactacc aggcgatctg cacctgggtt aagatcgcgt tttctttatc
     8881 aaaatcttta ttgttcgggg cccctccatc gaaaatgccc cgttcgtaat tcaattgggc
     8941 tttgacccgg tgccccaaat gccagttaat tcccaccgtc cagaccctgg cttttctcgc
     9001 actgaccgaa ggatcggcaa acgccgggaa ggcttcccga tcgaccagca cttccccata
     9061 ccgggccgcc agttccacag ctccccaggc tccttgagcc gggttgaaag ttcgccaggg
     9121 cttcaccccg ttcagcgacg catgttcccc ggtgagcaca tacgtcgccg ccacctgcca
     9181 ggcccgattc gtgagacgtt tttctgtgcc tcccgacgtc cgaagatcct ggatattcca
     9241 gacgtattcc ccgagcaagc cgaacggtcc ccaggagtaa taggtttgcg gtgtcaatcg
     9301 ccaccggtct ccgttgacgg ccactccatt actccaccgg aagaaggtaa taccatcctg
     9361 ccccatggcg ggagtccgaa aattcgacgg gctaaaatta tcacggcccc aggtgccggc
     9421 cacgcctacc tccaggccct ggagccattc cacgtcagtg gtcaggaagg gcttggtcat
     9481 cagccgcacc acgtaatctt tatcgttatt cgaatcgaaa tccgcattag atccgcctga
     9541 gataggggta ttgtcccgca cgccgttaaa aaccccggcc tgataatcca ggcgtttgtc
     9601 caacaaactt ccatgcaaca tcaccccgat atcccggttc ggcaggagat tcatcgtcat
     9661 gccgcgttcc acgaacaaca ggtcggcgcc ggatgcccac atttccaacc ccacgggtgg
     9721 tttaaatttg cctgcccgga accggactgc agaccagtaa tcgaacccga tatacccatc
     9781 gaacaacacc acccgcccct gctgaaagtc aggcatgacc ttgaatgtga cgtagcgata
     9841 caaggtcccc tcgaaaatcg gccggaagcg gcggacctcg aaagtatcgt gaacatccgc
     9901 atcgtcggtg tcgcccaaaa aggtccgcag ctgaatctgc ccgtaccccc ggaagcggag
     9961 ttcaaatgcc cggttagccg accgcagaaa aaacccattc tgatccgctc ctacaacggg
    10021 cctctccttc gccacttgtt cttcccgctg ttgatccagt tcccgaagcc ggtcgctaat
    10081 ccgttgcttc tgttctaatt cttccagccg ttctttcagg gttttgtgac ctgacgaacc
    10141 ttggggttct tgctcccgtg aactcgtgac ttctggctcc gccgcgaaaa gcccaccggt
    10201 tccactcatc aacaccatga ataatcctcc gataaccagc tcccgaaaca ttcgcatgcc
    10261 gtcctttcca gcatgagaac ttccggtggt ccggtcagtt cacgcttctc gctctttgat
    10321 tgattttgga attcgacggg ctccagtggg ctggtggccc ctgccgaatt cagtgttgcc
    10381 tcaggtcatt cactcccgtt cgtttttgtt tttgtgcgat tcccaccaag tcgaactttc
    10441 tccttccgct cgatttggac gatctctgaa tcgtggacaa caacttccac cgatccgtaa
    10501 cgaaggctct gaatagcctt gaaaatttct tcttggatgt ggctactcaa atccatcagt
    10561 cgcatcctct cttcccaagc tagccataca acatttcata taaatacata aactctatgg
    10621 ttttagtagt ctaattggat aaaaaaatat taaatactga acatcaacgg ggaatccttc
    10681 ccctgtttca ccatctgaat gtcccttacg acatccgcca aactcatacg atccaacaca
    10741 cttgccatgg catcccgcac ctctttcatc atgattctca cgccacaggt cttttcgtca
    10801 ggacattctt cacacttttg ataagccgtc tggctcacac agagggtcgg cgccaatgga
    10861 ccgttgagaa tacgaacgat ctgccccatc atgatttgac tgggagcctt gctaagcgca
    10921 tatcccccgc ccttgccttt tttgctttga aggattccat ggttttttaa atcgagaagg
    10981 atttgttcca gaaatttttt gggaatccct tcttgggtgg ctagttctga aatcaagacc
    11041 ggttcgtttt taggttgctg tgctaaatag atcaacgccc tgagaccgta tttggtcttt
    11101 tttgaaatca tatgaaatac ataaagtcta tagtttttat aggttaataa aatcattcat
    11161 ccctgtcaag agaaaatatc acgatatttg gaatttttaa tggtcagagc actgagaagt
    11221 ggtggccgtg caatggccca cgacgcaagc cgaaagtgga caggaatgga ctccaaccaa
    11281 aaacctcact aaccccaagt aagctgttca ataatttcag tccccctcgc gggcgaatga
    11341 ggggctgact gagatccctt tccaatcctg ccacaccctt gaaataattt ttgaaagaaa
    11401 tgagaaaggt aatcggatac cggaaaaagt gctgggaaaa tgagaagcgc cgagaaacat
    11461 gcgaggccac atcattccca acctccttac gcaaatgaga gacctcaacc tttcaccgaa
    11521 aggttcctcc cgacggttat tgattttccc ccttgactgg atagtgctca atagcaccgg
    11581 tttaccaaga cattatgaaa tgttaccggc cgctaacggt tcggaaactt catggtaaca
    11641 gtactctttc atgatactgg aagaagcaat ccccgatcat acatgatatt cagcaggaga
    11701 cacacaatgt aactcatggt cattactctg ggaatactta tactccttca gccgctcctt
    11761 tacgcgattc gcactcttct ccatcttccc agacgcccac gcctgtgtta tccgattttg
    11821 catggcaagg gcccgaaggg gaaattctcc ctggtggcct aaccatcgat ctcaagtagg
    11881 acgaggaaat tcaaatctcc ctattagtaa agaacccccc agcaagctgg gaggcattta
    11941 agatttagaa attcaattcc agttgcttgg gatcatgttg atgcttgata gatcgacgaa
    12001 tgacctcaac cgtgacgttg tctcctacac tccggacaaa atagccgtcg ttccacaatt
    12061 ctccgcccca caattgctgc tttacctctg gaaactcctg aaaaactatc cgagccgaaa
    12121 tacttttcat cctctgcaca acttgagcag gactatcgcg tggggggact gaaagaaata
    12181 tatgtacatg atcatccaag acttcttggg tatcgatttc aaatccatac cgttcggcaa
    12241 tctcctgaaa gatcctcttc aaccgacgcg ccactcgctc acccaagacc attcgccgat
    12301 atttggggac ccacaccaca tgatctttga gatcgcaaac cccacctcgg gtccgcttga
    12361 gactcatgcc tcgaaggata cctaattcag cccgctctgc gggcttgctc ctttcctccc
    12421 ccagcaagct tgggggtatc caggagcaat tttttatgaa tgtgataaca aaaggagcat
    12481 acttatggtt cgagtaaaag aactcatgaa acaggatctc gcgtcagtga attgcggaga
    12541 cctcgtgtca ctagcagcta ccttgatgcg gattcgccgc atcggtagcg tgttcgtcaa
    12601 acaaaacgga catattgtgg gcatcgtcac cgaagccgac attgtccgca aagtggtctc
    12661 gacccaccgt tcgcctgacc ggaccacggt cgaaaccatt atgacggctc ccgtaatcag
    12721 atcaagatgc gccgatattc gaagcggcgg atgtgatgta tcgatccggc atccgccatc
    12781 tggccgtcac ccaccactat gacattgtcg ggatcgtgtc ggttcgcgac ctcctgcatc
    12841 cagtggccat cgatgaattc taatattccc ctaccgacgc tggctccaaa ccttcaggaa
    12901 tcacacactg gaacccgatc caacaggaat gtgcaagact ttcaagcggc cctcaacgga
    12961 cttattctcc ggggtgtagc ggaaagggcg acacaaccat gccgaatttg tggcgattgt
    13021 ccctgaaggt caggtgaact tgagatgatc gggaccggca gccctcaaat gggtatttcg
    13081 acaaatgcgc tatttctgac cgaaatacca gcctttggac ttcctgtttt gcgccgatag
    13141 ggggtttcat tgaaccaggg gataaaccgc atcaagtttt tccgcctagg ggaaggcagc
    13201 gggtcttcaa gatgggattg tcgggtttcc gacctgggca gagattacgt gcagcgaata
    13261 taaagcagta ttatcagtga tccaggatgg gcaacaatcc aacgaaatta ccttctgaaa
    13321 tgattggttt gtgaaaatgg acgtggaaga ccggccgcaa ggcaagctca tgttttaatc
    13381 gaatacaccc catatcaaaa cggaccatct caggaatttt tcgtactgag actctcatac
    13441 acatgcgcta tgccttgctt cttatatctt tcaactcctt acgaaattca tccattccag
    13501 catgtacggc ttctttaatc cccgcctgaa tgtcttccaa ccccttacgg gtcccggaaa
    13561 cgatgtcttg atacgtctca acggttctat cctgcagatc gttcaaatct tctccttttc
    13621 gacggacata atcacgaatt tgaccacgtg attcccgccc ggattgcggg caaagcaaaa
    13681 acgtcgccac ggttccaatg actatccccg taatgaacac actgaccatt tttcctggtg
    13741 tggcatctct gtcgtattgt ttcatggagt ctccttggtt aaatgaagaa ttttttgtgg
    13801 aaaaaaaaag atcgggatac tcaaaatcat cgaacggttc gtactggaac catgtggcct
    13861 gcttattaac tatcatgagg aaacttttct gtgaacggac aaatccggtc ggatcttcag
    13921 gaagaagtcc agaagaaccc cttccggaaa aatgatagtc acagagaatc gtaaagacat
    13981 atcatgatct cctcgggatg acacggtagt atgcgggtaa gtgttccgac aacagccgtg
    14041 tgacaactga tcatacccct gatttctcag ggccgactta gacttcctgg gctcccgggg
    14101 gttcctgggt ccataccttc cagtacgcac agattcgttt actgaaatcc ttcggggatt
    14161 cgacaaagag acggcatcct aatggacgga cagccgttcg caacatctgt tttcgttgat
    14221 gaatcactcc aatgaggatg gcatacgaac agtcagaaga acggggtact tcacgtgatt
    14281 ctgtcaacaa tctttcgaag acgctcaaat catgatcttc tccgagatac tcctccaagc
    14341 gatggagttc agaggatcgt cccgaaaaga caggttgcca gacgtctctt accaaacgga
    14401 aatgatacca gtgatacttg acatgcttcc gccaatcatg aaacccctca tcggtcggcc
    14461 gggtacatac gtctgacatc gcccgaaaag ctttagcata cgttgttttc aatccttcat
    14521 acatgacggg ttcacggcag tcattgagca gggcatcaga aatatgttgt cgacttttta
    14581 gcaaactggc ttgaatttct tgaaatgact gctcgaggga agacctctcg tgcaatagat
    14641 tttcaaaacg ggaggataac tgtcgacgga ttggaccgaa ttccctccgg tcaatctccg
    14701 gtctttgaaa tgccatcaga tgatcatagg tattgatcat gacttgagcg tcccggatat
    14761 cggataacat gcgggccgca tctctcaaac gactattttc ctgttgatac gtttttcggt
    14821 ttttcatccc ggtcttcgcc aaccgaataa gtcctctgat ttttttacat cgcttccgca
    14881 cctgatgtgg cacagtgatc ggatcgatag gacgccggtc cagttcctcc gacgccaggt
    14941 caatttgttc attcacaatt cgcttaatgc cttgaatgaa cgattccgag cgggaaagtc
    15001 gaaaagacat ctccgtaaca ccacctaatc aaatcaaata gaggagcaga aaagattcat
    15061 tgccacatag gcaccaatca ctcctagcaa aaccgaatct gaactcgtga atatacccga
    15121 tttgagataa ctgaccagaa accggtctga acctgtatca tcaagagagc gccggaaaaa
    15181 cctccaaggt ccatagtaga agggtgaggg ggaaatgatc gatcggcgtc aaacataaag
    15241 tatttaatga gatttcccca atcaggagag gtgccctctt acggtcccgt cagtacaaac
    15301 tggtgtttac tgcgctcagg aaaccagcat tttccccacc aggcggaatt tcagggtcgg
    15361 aattattacc caaaaaccag ggatctatac agttgtcatg agggtgattc cactttaggg
    15421 tgacatcaga tatgcaaccg aatctccaat tctctgtcct agggagtcca accccctacc
    15481 catgtgctta acgagagaag aggtgtgtta tgccacagag gacaaaaaaa cccactcgtg
    15541 ctgtccatac catcgagatt gaaattgccc acataatgaa tcaatcgtcc tgtgatcgat
    15601 gtggaggtct cctgattaag ggacattgtt tggatgtagc caaccccgaa ggagaattgt
    15661 ggattaccac caaacactgc atacagtgcg gcaatgtaat tgatccggtg atcttaaaga
    15721 accagaagga agtgttggat ccgcctcccc aagcggagcg ggtgccgcgc ccacggccga
    15781 cctttggagt ggccctccag agacggaacc gctaaatttc aaaagctccc caaacagatt
    15841 cccaaacggg gtcctccagc tccggcaacc actcatctcc tcgcatccta ttaaagaggg
    15901 aataccttaa cttcgtgcga ctttttcaag tccctcgatc acattttgac aattcaaaca
    15961 ggctttcgcg aaaggtaaag ccttgaggcg ggcattactg accggctctc cacattcatt
    16021 gcaaaaccca taggtccctt cccgaaggcg ggtcaacgct tcatcaaggg tcaccctcat
    16081 ccgatgacgg atttccagta aggaaaggag ttgatcggca gaactatcgc ggtaagccat
    16141 atcctgatca tccatggccg gttcccctcg ccaccgttcc tgttcttcac gacgctggag
    16201 aatcagatca tcaatttgtt tttgcacctc atttttcttc tctaacagca tttgccgaag
    16261 tccttcgttt cgcccatcag aaacaacttc gtgagccatc ttcctcatga cggtaactcc
    16321 tttgttggta tgagccttgt ggtattgcac cgatacgtgc cgtcccaacc agataattca
    16381 tcgcgcataa aaaaaactct accggaatgt aatgctttcc tgttcatccg aacactgcaa
    16441 ggaaccctgg aattacctaa aaatacactt gagtcccatt cagatgcatc catgtcccgc
    16501 tcattctttt cccccaaggg tgataagaga ctgagacaag tgcaatatga atatccttcg
    16561 tgagtctgac taatcccata tgcaaatcac ccatgaattc tcctgtaaat tcagcccggc
    16621 aattagcatg aaaaggacag aggcgcacag cgttctcctg cctgattaac aaaactcctg
    16681 tccggatctt tcgttatgcc agaatagggg gtgagagccg agccattttc caggcaaccc
    16741 aacagccgga ctgtcaggaa acgagaccgg ttattgcata cgccgattca gatcatatgc
    16801 cgcgctgatt aaggacaaat gagtcagagc ttgaggaaaa ttacccagat gcgccaggcc
    16861 gggacctaaa ccttcgccga ataatccaag atgattggca tgacccaaca ttttatcaaa
    16921 cagaaagtgg gcctttttga cgtctccggc gcgagataaa cactcaatgt accaaaacgt
    16981 acacatcgaa aaacttccat tctcc
//
LOCUS       DATIAL010000522         7410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_92788, whole genome shotgun sequence.
ACCESSION   DATIAL010000522 DATIAL010000000
VERSION     DATIAL010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92788"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VLA60_16110"
     CDS             <1..541
                     /locus_tag="VLA60_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10936.1"
                     /translation="TQFLNLEFHLLGKLRLDDVVYDAYARHLIPRAVGYSAAVLDYFF
                     RGNIPLHKDKDAYEYFIARHYVGGPLDEEGYLDINFDIPRDVAFEGSTYLYIDLPGGQ
                     RILGSESFWDEYYRPFMFTYPDGAINFSLFTSIPRSQLDQSFRWTIVWEGKGGPGAQE
                     PRIIIGQTGMATWKEICVC"
     gene            1343..1543
                     /locus_tag="VLA60_16115"
     CDS             1343..1543
                     /locus_tag="VLA60_16115"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HSF10937.1"
                     /translation="MPKNLALDDKLVQEVQRLGGHSTKRAAVNEALREYVTRRKQKKI
                     LEIFGTLEWDPTCDYKTARKRK"
     gene            1651..1941
                     /locus_tag="VLA60_16120"
                     /pseudo
     CDS             1651..1941
                     /locus_tag="VLA60_16120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
     gene            complement(2262..2873)
                     /locus_tag="VLA60_16125"
     CDS             complement(2262..2873)
                     /locus_tag="VLA60_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10938.1"
                     /translation="MNERLPGIFHWRVTHPDIQIEVDSYYVSCLNPAFLIDPLVPQEG
                     TDWFRERPAPQHIYLTNRLHDRHCRQFIKNFGVTVWCHEAGLHEFADGNLAVTAFRFG
                     DEVPGGVRALKVGILCPEETALLLPSAGGVLSIGDAIINEDGELGFVPDTLIGDDPFS
                     VKRGLKAAFLQICQQETFDHLLFAHGDPIIGGGKDTLRAFCQG"
     gene            3452..4540
                     /locus_tag="VLA60_16130"
     CDS             3452..4540
                     /locus_tag="VLA60_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HSF10939.1"
                     /translation="MTQLHLLTPFQLGDLHLKNRVILAPMTRARAGKARLANALTAEY
                     YRQRSSAGLLITEATTISSQANGWNESPGMYTVKMRDAWKQVTDAVHAEGTPIFLQLW
                     HMGRASHSSFHDGELPVAPSAIMLNGDAIHTPLGKQAYEVPRALATEEIALVVADYQR
                     AARYAKEAGFDGVEIHGANGYLIDQFLQSRTNVRTDRYGGSIESRFRFLKEIVTAVCA
                     VWPSQRVGVRLSPNGNFNDMGSPDFREQFTYAAAQLDACNLGYLHIVDGLGFGFHELG
                     PPMALREFRSVYQGALMGNCGYDKSLADRAIREGRADLIAFGRPYISNPDLVERFRLN
                     AELNEPADMQHWYLPLGAKGYTDYPSLS"
     gene            complement(4748..5260)
                     /locus_tag="VLA60_16135"
     CDS             complement(4748..5260)
                     /locus_tag="VLA60_16135"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HSF10940.1"
                     /translation="MTQAHIPKQKFFVDAMLGKLARWLRILGYDTAYERNIADEAIVD
                     RVLNEHRWLLTRDRFLVQRKAVRGRFTLIRSDFVTEQLRQIRTELGIRLAIDEETVCR
                     CVACNHILENIPPAQAGPRVPPFVAKHHTHFTGCPNCGQLYWAGTHWSHLQQQLKLLS
                     QAATNQVPDQ"
     gene            5523..6449
                     /locus_tag="VLA60_16140"
     CDS             5523..6449
                     /locus_tag="VLA60_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10941.1"
                     /translation="MNRFLCLVISFGFWALSNLIPSTLVAAEDPEEVPRLPLAMEVFL
                     KKPGKHLYELHVHLTNISDEPVSVDVRDLPWNPTDGSTWLSATRLDAQKSPIKQNLPL
                     GKFGSQMVPLVPGESIQNRVALNPRIPSLLDDIEQAGVQLQWDCPPSNLKFVCKPGSP
                     NTLTIPKGDPGQPDVYAIDPQACLALERRIGLIDIPQGHDVLFLLASESLMTDLEGMT
                     SLLLDVDDYVQRCHPSWTNSWAVSFVTDKKFAGFLSEGENRRYFAEGLWQEANIGQYS
                     SQIRTLFRFQWIKKRADTVYLSLFPFQQQNDT"
     gene            complement(7009..>7410)
                     /locus_tag="VLA60_16145"
     CDS             complement(7009..>7410)
                     /locus_tag="VLA60_16145"
                     /inference="COORDINATES: protein motif:HMM:NF023318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3410 domain-containing protein"
                     /protein_id="HSF10942.1"
                     /translation="HIAGHSLDGKANGTFMIYAALCKHLGISPTWNPIKVLPTPTVPS
                     LTIDYHEQSDEELIQEMVSTIYDLEADYHRMQQLLEMPQEDRPRRFDELRKNYPVRRE
                     FHRTNITLPKNQERLQHLLSEIGFMEFSEEA"
BASE COUNT         1766 a         1810 c         1960 g         1874 t
ORIGIN      
        1 gacgcaattt ctcaacttgg aattccatct tttagggaaa ctgcgactgg atgatgtagt
       61 ttacgacgcc tatgccagac atcttatccc ccgcgcggtg ggctattctg cggctgttct
      121 tgattacttc tttcggggca atattcctct ccataaagat aaagatgcct atgagtattt
      181 tattgctcgt cattatgttg gcgggccctt ggatgaggag ggatatctag acataaattt
      241 tgatattcct cgagatgtgg ctttcgaagg aagcacttac ttgtatattg acttgccggg
      301 tggacaacga attctgggaa gtgaatcctt ttgggatgag tactaccgtc cctttatgtt
      361 cacatatcca gatggagcca tcaacttttc actttttaca tccattcccc ggtcccagct
      421 ggaccagtcg tttaggtgga cgattgtttg ggaggggaaa ggaggaccag gagcgcagga
      481 accccgaatc ataattgggc aaaccggcat ggctacatgg aaagaaatat gtgtgtgtta
      541 aagggaagct gtctatccca accactgcga atgtttctat ccgctgatga cgtctcgaaa
      601 gacaatgaat ggcgtcgaag tcgggatcgt gccataaatc gcgaaacagg tgaagagtta
      661 cttgtctacc tctaagagga agggtttgtg tgggaagcgg atcatctatg atatataaga
      721 aattttgtga acatggattt tttctcagtt cctttcctgc ccaacaatga attctcgaat
      781 cctgtattcg aagatctcga cacttacagg caatttgaaa aatatgccaa ccagtgcccg
      841 agaaaatttg cattcttcgt tctttacccc atccccattc ctccgcattc accctatagc
      901 cagaggtgga aacggtatcg ctcacccatt aagtcctcgg cgccttcgtc aggcgccgac
      961 gggaaggcgg agtgatcatg taccgaaatc gagcagcagg aatagaaaga attaacccat
     1021 cctgctacca acggaaaagc cagaagaacc aaattggttc attcgacttt tgcatcggtc
     1081 acgcgaaccg aataaaagtt aaggtagccg cagtggaaat aacggggtct gttcctgata
     1141 aggcttttgg gggaaacact gaggaatggg cattcaggga aacgagaaag gcaggctcct
     1201 cagcccactg ggtcgcaatc gattttggtt tggcaatcaa atctctgctt actaggtaac
     1261 cctttgactc actggaatat tgcccaccat ggacatgttg tgtagcataa tgtgtagatt
     1321 tttgtgggaa aggagggata tcatgccaaa aaatcttgct ctcgacgaca agcttgttca
     1381 agaagtgcaa cgtctaggag gtcattccac caaacgagct gctgtcaatg aagcactccg
     1441 agaatatgtc acccgccgca agcagaaaaa aatccttgag atattcggaa ccctggaatg
     1501 ggaccccaca tgtgattaca agacggcacg aaaacggaaa tgaccgtctt agttgattcg
     1561 tgcgtttggt tgggcgctct tcctccggaa agatgacccg gcaaaccacc ccgcagtcaa
     1621 aaattacaaa gcttcctttt agatacacaa aacattgtcc ttattgggat cattctgcag
     1681 gaaattctcc aaggctttaa acatgaaggg acctttacta aaggtaccac ctatctgaat
     1741 gcctttcccc tactctcctt aaaccgcagt gactttgttg cggcagccaa acttcgccgc
     1801 caagccgcag cacaagggtt gaccctctcc acccctgatt gccaaatcgc cgcagccgcc
     1861 atccaacacg actgcaccct cttaaccacc gacaaagatt tttcgaatct tgctcaatta
     1921 gtgcccctgc aattggtcta gccctcttct gtatgatcgc ctcggccgtc aagcctgggg
     1981 aggccagttt gtttggcggt ctgattgggg atatcaagga tggtgaccag gctgtggaaa
     2041 agaaatggtt tatttgatca caattgctgg ctggtcaagt caagaacgat tctcgcaata
     2101 agtgctccca aaataggccc actacttttg atatctttcg tttgtgggta tccttccttc
     2161 atttgttcag taggacctag atattgacca tggttccggt gctggaaaca gtgcggagaa
     2221 gggagttacc tccaccaata agaggggagt cgaacagtgg ttcagccctg acagaacgcg
     2281 cgaagcgtgt ctttgcctcc accaatgatg ggatccccat gggcgaacag cagatgatcg
     2341 aaggtctcct gctgacaaat ctggaggaac gcggctttta agccacgctt gacggagaat
     2401 ggatcatcgc caatcagcgt gtcaggtaca aagccgagtt cgccgtcttc attgatgatg
     2461 gcatcaccaa tagatagcac accgccggca cttggaagca gcagagctgt ctcttcaggg
     2521 cagagtatgc caactttaag cgcacggaca ccgcccggca cttcgtcgcc gaaccgaaat
     2581 gccgtaacgg ccaagttacc gtccgcaaac tcgtgcagcc ctgcctcatg gcaccagacc
     2641 gtgacgccaa agtttttgat aaattgtcgg cagtggcggt cgtggagccg attggtcaga
     2701 taaatgtgtt gcggggccgg ccgttccctg aaccagtcgg tcccttcctg aggtaccagc
     2761 ggatcgatca gaaaggccgg gttcaggcag gagacgtagt agctgtcgac ttcaatctgg
     2821 atgtcggggt gagtaactcg ccagtgaaaa attcctggta gtcgttcgtt catgagatgt
     2881 catcatcggt tcggccgggc ctgttcacag gacctggtgt gtgggcatga gagagatgtg
     2941 tatcgggatg ccattttgag ttttgccagt taaacagtgt tggcagatga acgaattgta
     3001 cttgtgtcac caattctcgt gtcaacgtgc ttttctgtgt tgccgggttt cgccccggca
     3061 ggcgaggccc ttttgttccg gcaaaaggac ccaaaaccat tgtcgccccg tctggcttcc
     3121 ttggaaaggg cggacgccaa cctgcgaagg gcggaccaac tcgatccgct caaacaaggt
     3181 ccgccagcgg atgagagcgt ccctcccttg ggccagacgg caggcgtcgg atttggagag
     3241 ttgaacattt cagtgacgca catgaaagag cgaggagccc tatattctgt atgatccaca
     3301 atactttcag acctgcgcgg actggcggtt agcatggaca gtcttgagcc tcattgaaat
     3361 agtctcacat catgaaatat aaaccatacc atgcgaatct cacaattgcc cgttagatac
     3421 ccgtatctca ctgtaaccct ggatggaaaa aatgactcaa cttcatttgc tgaccccatt
     3481 ccaattaggt gacctgcacc tgaagaatcg ggtcatcctg gcgccgatga ccagagcgcg
     3541 agccggaaaa gcacgattgg cgaatgcctt aacggctgaa tattaccggc agcgaagttc
     3601 ggcgggatta ttaattaccg aagccaccac catttcttcc caggccaatg gctggaatga
     3661 atctcccggc atgtacacgg tgaagatgcg ggacgcgtgg aaacaggtga ccgacgctgt
     3721 ccatgccgaa ggaacgccta tctttttaca attgtggcat atgggacggg cttcacatag
     3781 cagttttcat gatggtgagc tcccggtggc cccttcggcg attatgctca atggcgatgc
     3841 gattcatacc cctctaggga agcaagccta tgaggttccg cgcgcgctag ctacggagga
     3901 gattgccctg gtggttgccg actaccagag agccgcccgc tatgcgaaag aggcggggtt
     3961 cgatggcgtg gagattcatg gggccaacgg gtacttgatc gatcagtttt tgcaatcacg
     4021 gaccaatgtg cgtaccgacc ggtatggcgg gagcattgaa tcccgctttc ggtttttgaa
     4081 ggaaattgtc acagcggttt gcgctgtctg gccttcacag cgcgtggggg tgagactctc
     4141 tcccaatggc aattttaacg atatgggatc accggacttc cgcgaacagt tcacctatgc
     4201 agccgctcag ttagatgcct gcaatctggg ctacctgcat atcgtggacg gcctgggctt
     4261 cggcttccat gaactcgggc ctcccatggc tttgcgcgaa tttcgttctg tctatcaggg
     4321 cgccttaatg ggcaattgcg gatacgacaa gtcattggcc gaccgtgcca ttagagaggg
     4381 tcgcgccgat ttaatcgcgt ttggccgccc ctatatcagt aatcctgacc tggtggagcg
     4441 ctttaggctc aatgctgagc tgaacgagcc cgctgatatg caacattggt atttgcccct
     4501 gggcgcgaaa ggttatacag actatcccag cctatcataa taaatgggat gaacaggata
     4561 ctgtcagcgt acctggcttt gcatggatgt gtgtggggag gaacccactg tccgccggaa
     4621 gattagagaa ttctgtttgg cgatcgcatt gtaggagagt gtttgccact ttaagggccc
     4681 catgatgagg cattcgccat gcacaccttt ctccctcaag atgagtttct caactctatg
     4741 agatcagcta ttgatcgggg acttgattgg ttgccgcctg actcagcagc ttcagctgtt
     4801 gttgaagatg actccaatgc gtacctgccc agtagagttg gccacaattt gggcaaccgg
     4861 taaagtgcgt gtgatgtttg gcgacaaaag gaggcactcg tggaccggct tgagcaggtg
     4921 ggatgttctc cagaatgtgg ttgcaggcaa cgcaacgaca caccgtctcc tcgtcaatcg
     4981 cgagcctgat cccaagttcc gttcggatct gccgtaattg ttccgtcacg aagtcgctgc
     5041 gaataagcgt aaaacggcct cgaacggctt tccgttggac cagaaatcga tcccgggtta
     5101 aaagccatcg atgttcattc aagactcgat cgacgatcgc ttcgtcggca atgttccgct
     5161 catacgcggt atcataccca aggatacgaa gccatcgggc cagcttgccc agcatggcat
     5221 caacaaaaaa tttctgtttc gggatatgag cttgagtcat gatacaattt gcttttgggg
     5281 tcttccgaca ttcgcatggg tcaatatgca ggttgatcgt tccggaatcc gccattttac
     5341 aagggagagg gcttttcaca cagatctggt ccgcccgaat aatttacacg atttaccgca
     5401 gcaccagaaa tgatttgacc aattgtctga aaagacccta tcttgctcag ggtgtatgtg
     5461 aacggtgctt atagattata gattagatgg agatttgggg acggcttagg ggaggattaa
     5521 ttttgaaccg atttctttgt cttgtcataa gttttggatt ttgggctctg agcaatctca
     5581 tcccttccac actcgttgcg gctgaagacc ctgaggaagt gccccggttg ccgttggcga
     5641 tggaggtgtt tttaaagaag cccggtaaac atctgtatga actgcatgtt catttaacca
     5701 atatcagtga tgaaccggtc agcgtggatg ttcgggattt gccctggaat cccacggatg
     5761 gttccacgtg gctttcggcc acccgtttgg atgcgcagaa gagtcctatc aagcagaacc
     5821 ttcctcttgg gaagttcgga tcacaaatgg ttccgcttgt gccaggcgaa tcgattcaga
     5881 atagggtggc attaaatccc cggattcctt ccttgttaga tgatattgag caagccgggg
     5941 tgcaattgca gtgggactgt cctccgtcta acctcaaatt tgtgtgcaaa cccggttcac
     6001 ccaataccct taccattcca aagggcgatc ccggccaacc ggatgtgtat gccattgatc
     6061 cccaagcctg tctggctctg gaacgccgta ttggtcttat cgatattcct caagggcatg
     6121 atgtgttgtt tctcctcgcc tccgaatcgc tcatgacgga tctggaggga atgacctcgt
     6181 tgcttcttga cgtagatgac tatgttcagc gctgtcatcc gagttggacg aacagttggg
     6241 cggtgagctt tgtgaccgat aagaagttcg cgggatttct gagtgagggg gagaatcggc
     6301 gttattttgc agaaggcctc tggcaagagg cgaatatcgg gcagtattcc agtcaaatcc
     6361 gcacgttatt ccgatttcag tggattaaga aacgagcgga taccgtctat ctttctcttt
     6421 ttccctttca acaacagaac gatacctaaa tgaaagttta gagctcgacc cttctgttgg
     6481 cagttcgact gtccgttctc cttcggcccg gctccggaca aacccttcga ctgccggggc
     6541 gtgttggcgc tcggggtgtc tagagataaa ggcatttcga tttgcttcca tttggttcgg
     6601 ggtgaatagc accaaaattt caggcacatt ccactaactg gcatattttg aatgtatgat
     6661 agcagccggg tttcggcccg gcagccgagg tccttttggt tcggcaaaag gacccaaaac
     6721 catggacgcc ccgtccggtc tcattccagg cgggacggac gcgagcttga ggagggcggc
     6781 ctaactcgct acgctcaaac aaagtccgcc ggtgcatgag agcgcccgac ccagggaccg
     6841 gacggcaggc gtcggccaat ggcgggggag tacacagaca aaggcgactt tggaaattaa
     6901 ttggaattac tatactcagt ctgccggtgc caagcttacc gagcaccttt gtgctgaggc
     6961 tttcgaagga taagcgggtg caagtcagtt gtcctgtcca attctgtttt atgcttcctc
     7021 gctgaattcc ataaatccta tttctgaaag caggtgctga agcctctcct ggtttttggg
     7081 gagcgtgatg ttggttcggt ggaattcccg gcggacggga tagtttttgc gaagttcatc
     7141 aaagcgacga ggccgatctt cctgaggcat ctccagcagt tgttgcatgc gatgataatc
     7201 ggcctccagg tcatagattg tgctgaccat ttcttgaatc aactcttcat cagattgctc
     7261 gtgatagtcg attgttaaag aggggacggt tggtgtgggc agtaccttaa tcgggttcca
     7321 tgtgggggag atgcccagat gtttgcacaa ggctgcgtag atcatgaagg tgccattggc
     7381 tttgccatcg agggagtggc cggcaatatg
//
LOCUS       DATIAL010000523         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1382637, whole genome shotgun sequence.
ACCESSION   DATIAL010000523 DATIAL010000000
VERSION     DATIAL010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382637"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1039)
                     /locus_tag="VLA60_16150"
     CDS             complement(80..1039)
                     /locus_tag="VLA60_16150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF10943.1"
                     /translation="MNIPSPAADPLILSSPVRIGWIGAGVMGAPMCEHLLRAGYSLTV
                     FTRTPSKAQVLLDQGARWAESPAAVAAEADVVCTMVGFPQDVRRIYLQADGLLPQART
                     GQVFVDFTTSEPTLARELASLAASKSAFALDAPVSGGDLGARDGTLSIMVGGDLMAFE
                     AVRPILSVFEKTVVYHGQAGCGQHAKLCNQITIAGTMIGVCEALLYAHKAGLDGETLL
                     RSISSGAAGCWTLDHLAPRILKRDFEAGFFVEHFIKDMGMALDEAKRMGLLLTGLSLV
                     RKLYVAIQANGHGRSGTHALLLALEDLSPTFFSADSSPHALSK"
     gene            complement(1063..>2061)
                     /locus_tag="VLA60_16155"
     CDS             complement(1063..>2061)
                     /locus_tag="VLA60_16155"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HSF10944.1"
                     /translation="LGWPHNTKDWPGKMGAISWVYGEMIRRLSLGESVRILVNDKDHE
                     LKARRVLDKVGVDMTRIQFFRVPTNRGWARDFGPIFVKRDQSDPQVAIARFRFTAWGK
                     YPDWQLDDRIPVRLGPKLKIPVVPVERNRVGVVLEGGAIDVNGQGTLLTTEECMLDEQ
                     VQVRNPGFSRQDYEEVFGAHLGISQVLWLGQGIAGDDTHGHVDDVCRFVDPYTVVLCQ
                     ESNPADQNYRPLAENAERLEGMRVEDGRKLLVVPLPMPEPLYFEGRRLPASYANFYIG
                     NAVVLVPTFNDPHDRVAVGIMSDLFPDRRVVGIHAVDLVWGFGTIHCLTQQQPASD"
BASE COUNT          575 a          619 c          452 g          415 t
ORIGIN      
        1 ccgagcatta gtccagagcg ggagcgtgcc ccccatccat agctacaaac gattgaggaa
       61 aggcggatcg ggatttgtac tactttgaca aagcgtgagg agaggaatct gcagagaaaa
      121 aagtcggaga taaatcttcc agcgccaaca acaacgcatg cgtcccggaa cgcccatgac
      181 cattggcttg aatcgcaaca taaagctttc gaaccagtga aagccctgtt agaagtaagc
      241 ccattcgttt ggcttcatct aatgccatac ccatatcctt aataaaatgc tctacaaaaa
      301 atccggcttc aaaatcccgt ttcaatatcc ggggtgccag atggtcaaga gtccaacatc
      361 ccgcagcccc actggatatg gaacgcaaca aggtttcgcc atccaacccg gctttgtggg
      421 catacaataa cgcctcacac acaccaatca tcgtgcccgc aatggttatc tgattacaga
      481 gtttggcatg ctgcccgcat ccggcttggc cgtgatatac tacagttttt tcaaacacgg
      541 acaagatcgg cctgaccgct tcaaatgcca tcaaatctcc acccaccata atggacaatg
      601 tgccatctct ggccccaagg tcaccgccag aaaccggcgc atccagagcg aatgcggatt
      661 ttgaagccgc caaactggcc aattcacgag ctaacgtcgg ctcagaagtg gtgaaatcca
      721 cgaagacctg tccagtgcga gcctgcggca acagcccatc tgcttgaaga tatatccgac
      781 gaacatcttg cggaaaaccc accattgtac aaaccacatc ggcctccgca gctacggcgg
      841 ccggagattc tgcccaccgg gcgccttgat ccaacaacac ctgcgcttta gaaggcgtgc
      901 gggtaaatac cgtcaaggaa taccccgcac gaaggagatg ttcgcacatt ggcgcgccca
      961 tcacgccagc accaatccat ccgattcgaa caggcgaaga aagaataaga ggatcggccg
     1021 caggggaagg gatattcatg aacgctcctt gagcatggat aattaatcag aggcaggctg
     1081 ttgttgcgtc aggcaatgaa tggtcccaaa tccccagacc agatccacag catgaatacc
     1141 gaccaccctt cgatcaggaa acaggtcaga cataatccct accgccaccc gatcgtgggg
     1201 atcattaaag gtcggcacca acacgacagc attcccgata taaaagttgg cataactggc
     1261 aggtagccgc cgtccttcaa aatagagcgg ctcaggcatc ggcaagggga ccaccagtaa
     1321 ctttctcccg tcttccaccc gcatgccctc taatcgctcg gcattttcag ccaagggacg
     1381 ataattttga tcggctggat tggattcctg acacagcacc accgtatagg gatcaacgaa
     1441 ccggcataca tcatccacat gcccatgcgt atcgtcaccg gcaatgcctt gtcctaacca
     1501 gagaacctgg gaaataccta aatgtgcccc aaacacttcc tcataatcct gtcgggaaaa
     1561 cccgggattc cgcacctgca cctgctcatc caacatacat tcttccgtgg tcaacaacgt
     1621 tccttgccca ttcacatcaa tagcgccacc ttccagaaca accccgacac gatttctctc
     1681 cacaggaacc acgggaatct taagtttcgg acctaaccga accgggatac gatcatccaa
     1741 ttgccaatcc ggatacttgc cccaggctgt aaaacgaaaa cgggcaatgg ccacctgagg
     1801 gtcagactga tcccgtttca cgaaaatggg gccaaagtca cgcgcccagc cccggttagt
     1861 cggaacacga aaaaattgaa tgcgggtcat atcgacccca actttgtcca gcacccgcct
     1921 ggctttcaat tcatgatctt tgtcattcac aagaatccga acagattctc ctaaagacag
     1981 cctccgaatc atttccccat acacccagga gatcgctccc attttcccag gccaatcttt
     2041 cgtattatgg ggccatccga g
//
LOCUS       DATIAL010000524         5109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_522356, whole genome shotgun sequence.
ACCESSION   DATIAL010000524 DATIAL010000000
VERSION     DATIAL010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522356"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VLA60_16160"
     CDS             complement(<1..367)
                     /locus_tag="VLA60_16160"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HSF10945.1"
                     /translation="MKLLTPQDLLSAAQYEEDRAAIRQKIIALKKRRRISVGEFVTLV
                     FENRETLLFQIQEMIRIERIFDPGKIQEECDVYNALLPDRHELSATLFIEITDSENIQ
                     PLLDSFKNIDQANTVGLKVG"
     gene            complement(364..1638)
                     /locus_tag="VLA60_16165"
     CDS             complement(364..1638)
                     /locus_tag="VLA60_16165"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HSF10946.1"
                     /translation="MPKFDLITPGWTRSQLETETRRIFDVCDGCRRCFNLCPSFNTLI
                     DRIDEYESDSTQFTAQDFTKIEQECYYCKLCFNHCPYSPPHQYDLDFPRLMAAWKKQR
                     TAEGGATWRDKFLIQTDLIGKLGSLTAPLTNWALRTPWLRSLVERMVGVHKNRQVLSF
                     QSQTFTQWWDQRKPAPAFTHSQGRVAFFPSCLVTYQVTDIGKAAVQILEKNGIEVVVP
                     PNQQCCGMPRFDVGDTDGMAKIAGSQYRLFAPYIDQGYDIVIPAPSCSLMFKREYPYL
                     KPTPEMKQLSERTFDLCEYLMRLKREGRLSLDFQANPGRVAYQIPCHLRDQNIGFKSK
                     ELMELTGATVHLIEKCSGHDGAWSAKTEFFDLSMKIAKKAVREIQEEPFDVVASDCPL
                     SALQLDQALQTAPTQSTLHPIQVVRNAYGLTP"
     gene            complement(1736..2164)
                     /locus_tag="VLA60_16170"
     CDS             complement(1736..2164)
                     /locus_tag="VLA60_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HSF10947.1"
                     /translation="MGKALKGTKSHENLKAAFAGESQANRRYLYFARRADIEGYTDIG
                     GLFRDTSEAETGHAFGHLDFLKEVGDPATGVPIGNTEANLKASIEGETYEYTQMYPGM
                     AKTARDEGLEELAEWFETLAKAERSHANRFTKGLESLKQS"
     gene            complement(2148..2651)
                     /locus_tag="VLA60_16175"
     CDS             complement(2148..2651)
                     /locus_tag="VLA60_16175"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HSF10948.1"
                     /translation="MEISFNDIKQKFQGCGLKTTPQRTAIYDALFRSTTHPTAEELFA
                     EVAPQFPMMSLNTVYYTLGALRTCGLIHEVNIGHIRARFDANLSPHHHLICLGCQSIV
                     DVIDPRLNRLTSPAGIPKDFEITSYQVAFRGLCGSCRRRTGRSINHSSPGLHPTTVKG
                     GLHGKSS"
     gene            complement(2795..3727)
                     /locus_tag="VLA60_16180"
     CDS             complement(2795..3727)
                     /locus_tag="VLA60_16180"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HSF10949.1"
                     /translation="MSSRIVSLIPSGTEMVCALGCRAQLVGRSHECDFPSDITALPIC
                     TQATVDSEGKSQAIHAQVSERLQSALSLYKVLVDQMQDLRPDVIVTQTQCEVCAVSAD
                     EVQRALRDLIGMSPTLISLGAQDLSGVWDDLTRVADGLGQQAAGQAFLKQAHQRIGDI
                     ADRSQQQPTPPTVACIEWMEPLMAAGNWVPELVHLAGGQSVFGQRGTHAPWITWEALA
                     ASDPDILVLMPCGFSMARIQEELPVMTSHPFWPQLRAVQNQKVYLTDGNQFFNRPGPR
                     LVESLEILAEIFHPTHFHFEHQGQGWRQWVPGYP"
     gene            3989..4225
                     /locus_tag="VLA60_16185"
     CDS             3989..4225
                     /locus_tag="VLA60_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HSF10950.1"
                     /translation="MAAKAYILMKVKAGKVQDVLETLKALSGVEQAHACFGQPDIFGL
                     VNAPDDRALSNLIMAKIHTIPGVEETDTHIVVQD"
     gene            complement(4503..4982)
                     /locus_tag="VLA60_16190"
     CDS             complement(4503..4982)
                     /locus_tag="VLA60_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HSF10951.1"
                     /translation="MESLLAWGHFLAGITWIGILYYFNFIQVPFLKAVTPETKAEAFK
                     HLVPNALWWFRWGALFTWIFGAALLMNQHRMGSAFMLQGPDAVIGMGAWLGTIMLLNV
                     WGIIWPNQKKVLGLKEATPEEKAKAGRMALLASRTNTMLSIPMLFFMANSGHASGLF"
BASE COUNT         1261 a         1223 c         1342 g         1283 t
ORIGIN      
        1 cgccaacctt gaggcccacg gtattggcct ggtcaatatt cttaaaggaa tccagcagcg
       61 gttgaatgtt ttcggaatcg gtgatttcga taaacagggt tgcgctcaat tcatgacgat
      121 ctggtaggag ggcattatac acgtcacatt cttcctgaat ttttcccgga tcaaaaatgc
      181 gctctattcg aatcatttct tgtatttgaa ataggagggt ttcccgattt tcaaatacga
      241 gtgtcacgaa ttctcctaca gaaatgcggc gccgtttttt aagggcaata attttttgcc
      301 gaatggcggc gcggtcttcc tcatattgag cagccgagag gagatcttgc ggtgtgagca
      361 gtttcatggt gttaatccat aggcgttacg aacgacttga ataggatgaa gggtcgattg
      421 agttggtgca gtttgaagtg cttggtccag ttgtagtgcc gacagggggc aatcggaggc
      481 gacgacgtcg aacggctcct cttgaatttc ccgcacggct ttcttggcga ttttcatcga
      541 gagatcaaag aattcagtct tggcactcca ggctccatcg tgtccggaac atttctcgat
      601 cagatgaacc gtggccccgg tcaactccat caattctttt gatttaaatc cgatgttctg
      661 atcacgtaaa tgacagggaa tttgatacgc cacgcgtccg ggattagctt gaaaatcaag
      721 ggaaagcctg ccttcccgtt tgagccgcat taaatattcg caaagatcaa acgtgcgctc
      781 gctcaattgc ttcatttcag gtgtgggttt gaggtaaggg tattctcgtt tgaacatcag
      841 gctacaactg ggtgctggaa tgacgatgtc atatccctga tcgatatagg gcgcaaagag
      901 tcgatattga gacccggcaa tcttggccat tccatcggta tcacccacgt caaaccgtgg
      961 catcccacag cactgctgat tcggaggtac cacgacctca atgccgtttt tctccaaaat
     1021 ttgtacggca gctttcccaa tatcagtcac ctgatacgtc accaggcaac ttgggaagaa
     1081 ggctactctt ccctggctgt gggtaaatgc gggagccggc ttccgctgat cccaccattg
     1141 ggtaaaggtc tgtgattgaa acgaaaggac ttggcggttt ttgtgcacgc cgaccatgcg
     1201 ctcaaccaga ctccgaagcc acggtgtgcg aagggcccaa ttggtcagtg gagcggtcag
     1261 gcttcccagt tttccgatca aatcggtttg gatcaggaac ttgtcacgcc atgtggcccc
     1321 accttcagcg gtccgttgct ttttccaggc agccatgagc ctgggaaaat ccagatcgta
     1381 ttgatggggg ggagaatagg gacaatggtt gaagcagagt ttgcagtaat aacattcctg
     1441 ttcaatcttc gtgaaatcct gtgccgtgaa ttgcgttgag tcgctctcat actcatcaat
     1501 ccggtcaata agggtattga acgacgggca taagttaaag catcgtcgac atccgtcaca
     1561 gacatcgaaa atgcgccgtg tctcggtttc gagttgagac cgggtccagc ctggagtgat
     1621 aaggtcaaat tttggcacgg gttatggctt tgtttgagag tctgtgaaag agtgtataga
     1681 agggggcggc agccttcttt atcgaccgcc gccgtgtgcc ataatgccga ggctattagc
     1741 tctgttttag ggattccagc cctttcgtaa accggttcgc atgggatcgt tcggctttgg
     1801 ccaacgtctc aaaccattct gcaagttcct caaggccttc atcacgagcg gttttcgcca
     1861 tgccgggata catctgggtg tattcgtagg tctcaccttc aatcgatgct tttaaattgg
     1921 cttcggtgtt gccaatcggc acgcccgtgg caggatcccc gacttccttc aaaaagtcca
     1981 gatgcccaaa cgcatgtccg gtttcagctt ccgaggtatc acgaaacaga ccgccgatat
     2041 cggtgtagcc ttcaatgtcc gcccgccggg cgaaatataa atagcgtcga ttcgcttgcg
     2101 attcaccggc aaatgccgct tttaaatttt catggctttt tgtgccttta agagcttttc
     2161 ccatggagtc ctcctttgac ggttgtgggg tgtaaccccg gtgatgaatg gttgattgag
     2221 cgccccgtgc ggcggcgaca ggagccgcaa agtccacgga aagcgacctg ataactcgta
     2281 atttcaaaat ctttgggaat gccggctggg gaggtcaggc gattgagtcg gggatcgatg
     2341 acatcgacaa tggactggca ccctaagcaa atcaaatggt gatggggaga tagattggca
     2401 tcaaatcgag cccgaatatg gccaatattg acttcatgaa tcaacccgca ggtacgcaag
     2461 gctccaagtg tgtagtagac ggtgttcaga gacatcatgg gaaattgcgg agcgacttca
     2521 gcaaagagct cttccgctgt cggatgggtc gtgctgcgga agagtgcatc ataaattgct
     2581 gttcgctgcg gagtcgtttt taatccgcat ccctgaaact tttgtttgat gtcattaaag
     2641 gaaatttcca tatcgttcct gtttaggaat agttactact taacaaaacc ctaatgagac
     2701 ggtcaaccgg agtaccgccg gctgcacagg ggcaagccaa gcaaaaacga aattgggaag
     2761 acccgtgctt cggtgagttg cgtcttcaag gtatttaagg gtatccagga acccactgcc
     2821 gccatccctg gccttggtgc tcaaaatgaa aatgagtggg atgaaagatc tcagccaaaa
     2881 tttcgaggga ttcaacgagg cgcggccccg gacggttgaa aaactggttc ccatccgtca
     2941 ggtacacctt ctgattttgc actgctcgta actgtggcca aaaaggatgt gatgtcatga
     3001 ccggcaattc ttcttgaatg cgagccatgg aaaatccaca aggcatcaag accagaatat
     3061 cgggatccga agcggctaag gcttcccagg ttatccatgg ggcgtgtgtc ccccgctgtc
     3121 caaacaccga ttgccctccg gccaaatgaa ccagttcggg tacccagttg ccggcagcca
     3181 tcagcggttc catccattcg atgcaggcca cagtgggagg agtaggttgt tgttgggaac
     3241 ggtccgcaat atcgcctatg cgttggtggg cttgtttcag aaatgcctgt cctgcagctt
     3301 gctgtcctaa gccgtccgcc actctggtta gatcgtccca aacccctgat aagtcctgcg
     3361 cacccaggga aatcaacgtg ggtgacatac ctatcaagtc gcgaagggcc cgctgcacct
     3421 catcagcgct cacggcacac acctcacact gggtctgggt tacgatgaca tccggacgta
     3481 agtcctgcat ttggtcgacc aagaccttat acaaggaaag ggctgactgg agacgttctg
     3541 aaacctgggc atgaatagct tgactctttc cctcactatc caccgtggct tgagtgcaaa
     3601 ttggaagagc ggtgatgtcg gaagggaaat cgcattcgtg tgaccggccg acaagttggg
     3661 cacggcaacc taatgcgcag accatttctg ttccactagg aatcaacgac acaatacgag
     3721 aagacacaat atttccttga gggtggatag gatggggaga aataatcgct catcctacct
     3781 gaggaaggca gggcgtccgc aacttctttt tggttgcttg ggctgtttgg gagggagggg
     3841 gaagagaatg gaagctacct tccatctatg gtacaaaggt actttcaaca tggctcggta
     3901 aagcgttatc gattatgtct tttgtgtccc aacatcaatt tcactattga tcaccgaaat
     3961 ctgattcgca ttcatcaagg aggtgggtat ggcagccaaa gcgtatattc tcatgaaagt
     4021 caaagccgga aaagtccaag atgtgttaga gacactcaaa gccctttctg gggtggaaca
     4081 agctcatgcc tgtttcggtc agcctgatat ctttggtctg gtcaatgcgc ctgatgaccg
     4141 tgccctgtcc aacctcatca tggccaagat tcacaccatc ccgggagtgg aggaaaccga
     4201 cacccatatc gtcgtccagg attagttgct tccattatcc ttgccgtaga agagggggac
     4261 gaacttaaaa ggttggagtc agtgccaagg acgaattgag gattctggga taatagatgg
     4321 agtctagcaa ttttcgctct ggattgtggg ctctggcttc ctggttcata caacccctcc
     4381 aggcatttct agtgagccac gaaaaaagca agaatgctgt atagaaaaag ccggggtctt
     4441 ttgaccccgg cttttgtcgg catccagatt tttgatctga gattaggaag ggacgattga
     4501 gttcagaaca aaccggaagc atggccggaa ttggccataa aaaataacat tgggatggaa
     4561 agcatggtat tcgtgcggga cgccaggagg gccatccgtc cagccttggc tttttcttca
     4621 ggtgtggctt ccttgagccc aaggactttt ttctgattgg gccaaatgat tccccacaca
     4681 ttcagcaaca taatggtccc aagccaggca cccatgccga tcacggcatc cggtccctgc
     4741 agcataaatg cacttcccat acgatgctga ttcatcagta aggcggcacc aaatatccag
     4801 gtgaataggg ctccccagcg aaaccaccag agcgcattgg gcaccaggtg tttaaaggct
     4861 tcagctttgg tttcgggtgt gacggctttg agaaatggca cttggatgaa attaaagtaa
     4921 tagagaattc caatccaggt aatacccgcg aggaaatgcc cccacgctaa taaactttcc
     4981 ataaaatgtc ctcgtattcg ttaggttgat taattatgtc tttggaggtg atgaggtgtc
     5041 acaccagcat tttgaccagg ccgtacagga ccacagtcaa tacaaagcca gcagtgatgg
     5101 tgccagaga
//
LOCUS       DATIAL010000525         5388 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_93028, whole genome shotgun sequence.
ACCESSION   DATIAL010000525 DATIAL010000000
VERSION     DATIAL010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93028"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..648
                     /locus_tag="VLA60_16195"
     CDS             85..648
                     /locus_tag="VLA60_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10952.1"
                     /translation="MTQIPEQHPLRQLFGRLTERNFSEALGWPDFQVTEYVSNLLLEF
                     IHIDQLSRIKDHEGEPVETVVELLYAAESHSQQSSAEKERDIHRHIGDLTLFLAGLFP
                     EYCKRIKCSGLIHHKDFLVDYVKAGKRSYSIVATHQDPQGKNPPALFQTLSTNFELCV
                     AGLGFVRLDLDRFQDPSLNQAQSQLFH"
     gene            1102..2625
                     /locus_tag="VLA60_16200"
     CDS             1102..2625
                     /locus_tag="VLA60_16200"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HSF10953.1"
                     /translation="MTPELEKKLQSCPSLPSPTNVAIKIINLANEPEVDIDRIIKILH
                     CDPALASKILRTANSPLYPYSKKIENLHQAVMVLGLNATISLALSFSLIKSLQTHRKS
                     GLDYSLYWKRAILAGIACRVLGTTCGLTGIIEELYLSSLIQDLGMLALDQVFPDLYDH
                     PDLDQTCHASIIAHEKHCLAVNHAAVGSWLLRQWNFPDRIRLAIAGSDDSSRIPPQDE
                     RAKFAYCVSLSGKIAEIFLRESHSEYLQVVKEEAHNLLNLTPETLMEVLETTEDLIPE
                     TEKIFQTDLQTWTEPQAILEKARETLLLRSLQTIKEVQELQINKATMEAQFHNLEEIH
                     RNDPLTGTLARAYLDQYLDSSFKQALANEECLTLVFGDLDKFKFVNDTYGHQAGDSVL
                     QSTARLLLAQVRTTDMVGRYGGEEFVLILPKTSSPAAKIVCKRILSAFRNTTHEIAKN
                     QNIAVTISLGVATHTPDMPYPDITALLLAADKAAYHSKSHGRNQCTYYEKIKIEQPA"
     gene            2682..3602
                     /locus_tag="VLA60_16205"
     CDS             2682..3602
                     /locus_tag="VLA60_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HSF10954.1"
                     /translation="MAKPTIILTPKVLIHGGAGSRQPTHSQSVCLTEALVKGYECLKT
                     GHSSLDAVETMIRYLEESGLFNAGRGSLRQLDGIQRMDASIMDGQTLSAGGVAGLEGY
                     LHPICAARRIMTDTDHVLIVGSHANRLAGHFGLARLVHLKSSTPLRNKRSSRNTINPK
                     SQALYRQLGLYDTVGAVALDCAGRLAAGASTGGVAVMLPGRVGDTPLIGAGVYADDTA
                     GAISMTGLGESIIRAGMAKHLAILLGLGWTPVKAANYALKTLVNRIQGEAGCLILDPT
                     GRFAIRHTTPWMSAGYWNGRGKPVVKNRFP"
     gene            3712..4155
                     /locus_tag="VLA60_16210"
     CDS             3712..4155
                     /locus_tag="VLA60_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HSF10955.1"
                     /translation="MSSSTFHLSIPVKDIESTKKFYVEGLGCGLGRQSSVAITLNFHG
                     HQIVAHVTEDLGPQQTSIYPRHFGLICGTEEEWNELRNRAQTNSLIFFREPRRRFPDT
                     PLEHLTFFLEDPSHNLLEFKYYLNANAIFGEETFSQIGDAHEQHG"
     gene            complement(4193..4867)
                     /gene="bcp"
                     /locus_tag="VLA60_16215"
     CDS             complement(4193..4867)
                     /gene="bcp"
                     /locus_tag="VLA60_16215"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HSF10956.1"
                     /translation="MASKVKKAQPKKKGPLQSKPTVVAKKKAAPKRSSSKSQGLPAGS
                     RNPSTKIKTASTPKTKKPAQQSPRTAATSELQFGDPAPAFSLPDDTGRIVNSSELRGK
                     KVVVYFYPKDDTPGCTTEACSFRDGIQELRKSGAVVFGVSADSVSSHRKFSDKFQLNF
                     PLLSDESKAMIQAYGVWKEKSMYGRKYMGIERTTVVINEDGTIRSVFPKVKVNGHFTE
                     VLQALR"
     gene            5071..5211
                     /locus_tag="VLA60_16220"
     CDS             5071..5211
                     /locus_tag="VLA60_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10957.1"
                     /translation="MTLAPGINQAIEPVSQKALTPFADDLGCDLQSTSAIRIRLSFPQ
                     AG"
BASE COUNT         1394 a         1427 c         1224 g         1343 t
ORIGIN      
        1 tcttacatca ggccgataga catacataga aaccgacaca ccctgacgaa gggacattcg
       61 gtggaaagag agggaagggc attgatgaca caaatccctg agcaacatcc tctaaggcag
      121 ttatttggcc ggttgacaga acgaaacttt tccgaagcat tgggatggcc agattttcaa
      181 gtgacggaat atgtttcaaa cctgcttctc gaatttatcc atatcgatca actctcccgt
      241 atcaaagacc atgagggaga acccgtcgaa acagtggtgg aactcctgta cgcggccgaa
      301 tcccatagcc aacagtcttc cgctgaaaaa gagcgggata ttcatcgtca tattggggat
      361 ttgaccttgt tcctggccgg actcttcccc gaatattgca aacggataaa atgctccgga
      421 cttattcacc acaaagattt tttggtggat tacgtcaaag ccggcaaacg ctcctacagt
      481 attgtcgcta cccatcaaga tcctcagggc aaaaatccac ccgcactctt tcaaacgctt
      541 tcaacgaatt ttgaactctg cgtcgcgggc cttggttttg tccgattaga cctggatcgt
      601 tttcaggatc ctagcctgaa ccaagcgcaa agccagcttt tccactaagc gctggtcttc
      661 acggccgttt ccttagctcg tttctcaata tggtgaccca cctgcccagc cctcttcgct
      721 gcctcactgg attttccggg atggcacgtc ccatcatcca cagaatttgc aaacccgaca
      781 aaaacagcct agtcaaaatc aatgtgacag aacatcacaa ttatctcgag gccccatgtt
      841 tttcatttgc attttccttg atcccgcttt ctctcttcga gcgggtcaat caggaggatc
      901 gcctccaaat taaaaggaga gaaaacgatt ttcctctcac gcctattcag atgacataaa
      961 aatatgccga aatgcctatg atcggaaaac atcccggata tatcctcgtg ttcatggatc
     1021 ccggtagcat gatgatttat agatcttcgg atatagggtc tttcgagaca ctcgcgaaga
     1081 tcttctgacc aagagtcctg tatgacacca gaacttgaaa agaaactaca atcctgccca
     1141 agccttccaa gcccgacaaa cgtcgccata aaaataatca acttagccaa cgaacccgaa
     1201 gtcgacatcg accgtatcat caaaattctg cattgtgatc cggcgctggc cagcaaaatt
     1261 ctacgaaccg ccaattctcc cttataccct tattccaaaa aaatcgagaa tcttcatcaa
     1321 gcggtcatgg tcctcggtct caacgcgacg atttccctcg ctctgtcctt ttcgttaatt
     1381 aaatcgcttc agacccatcg aaagtctggc ttggactatt cgctgtattg gaaacgtgcc
     1441 atcctagctg gaatagcctg tcgtgtccta gggacgacgt gtggattaac cggaataata
     1501 gaagaattgt atctttcgag tctcatacaa gatctgggga tgctcgccct cgatcaggtg
     1561 tttcctgacc tgtacgatca ccctgacctg gatcaaacat gccatgccag cattatcgcc
     1621 cacgaaaagc attgtctagc cgtgaatcat gccgctgtgg gaagttggct cttgaggcag
     1681 tggaattttc cagaccgcat ccggttggct atagccggaa gcgatgattc ctcccgcatt
     1741 cccccgcaag atgaacgggc gaaatttgcc tattgtgtgt cgctgtcagg gaaaattgcg
     1801 gaaatatttc tacgcgagtc tcatagcgaa tatctccagg ttgttaaaga agaagcgcat
     1861 aacttgctga acctcactcc agagacgctt atggaggtgc tggaaacgac cgaagacctt
     1921 attccagaga cagagaaaat ttttcagaca gacctacaga cgtggaccga acctcaagcc
     1981 attctggaaa aagccaggga gacacttctc cttcgaagcc ttcagactat taaagaagtt
     2041 caggaactcc aaatcaacaa agcaacgatg gaagcccaat tccacaattt ggaagaaatc
     2101 caccgtaatg atcccctcac cgggacattg gcaagagcat acctcgatca atacttagac
     2161 tcctctttta aacaggcgct cgccaatgaa gaatgtttga ccctggtatt tggagacctt
     2221 gataaattca aattcgtcaa tgatacctat ggacaccagg cgggggatag cgttcttcaa
     2281 tccacagcgc gtctactgct tgcgcaggtt cgcaccaccg atatggtcgg acgatacgga
     2341 ggagaagaat tcgtgctcat tctcccgaaa acctcatcac ccgccgctaa aatagtgtgt
     2401 aaaagaatcc tctccgcgtt tcgcaataca acccatgaaa tagctaaaaa tcaaaacatt
     2461 gccgtcacca tttctctggg agtcgcaaca cacactccgg atatgcccta tcccgatatc
     2521 accgcccttc tccttgccgc agacaaagcc gcctatcatt ctaaatccca cggtcgcaac
     2581 caatgcacat attatgagaa aataaaaatt gaacagccgg cttaacactg gaagcggtca
     2641 cgacccgtca ctccactctt ttttattaac tccttcgtcc tttggctaaa cccaccatca
     2701 tcctgactcc aaaagtcctg atccatgggg gcgcgggctc ccgacaacca acccacagtc
     2761 agtccgtttg tctgacagag gcactcgtaa agggatatga atgccttaag acagggcatt
     2821 catcattaga tgcggtagaa accatgatcc gttacctgga agaatcggga ttgtttaatg
     2881 ccgggcgagg atcgcttcga caactcgacg ggattcaacg gatggacgca tcgatcatgg
     2941 acgggcagac cttatcggct ggcggtgtag cagggctgga aggatatctt catccaatct
     3001 gcgccgcgcg acggatcatg accgacaccg accatgtatt gatagtcggc tcacatgcca
     3061 atcgattggc tggccatttt ggtttagccc gactggtcca tctgaagagt tcgactccac
     3121 tcaggaacaa acggagttca cgaaacacga ttaaccctaa atcccaggcg ctttaccgtc
     3181 agttaggatt gtatgacaca gtcggggcgg tcgctctaga ctgtgccggc cgtcttgccg
     3241 caggggcctc aacaggggga gttgcggtca tgttacctgg ccgtgttgga gataccccct
     3301 taatcggcgc gggagtgtat gccgatgata ccgccggggc tatttccatg acaggcttag
     3361 gagaatccat catacgagcc ggcatggcca aacatctggc tatcctgctc gggctaggct
     3421 ggacgcccgt aaaagctgcc aactatgcac tgaagacact cgttaaccga atacaggggg
     3481 aggccggatg cctcatcctc gatcccaccg gcaggtttgc gattcggcat accaccccct
     3541 ggatgagtgc cgggtattgg aacggtcgtg gtaagcctgt cgtcaaaaac cgttttccct
     3601 gaaggggggt gcgtaaaatg aaacgctcaa tcttagccgt tcatcgacaa atagcgccat
     3661 attttcattg atcatccgaa acatgcgata attcctgaag gagtctatta aatgtcctct
     3721 tccacctttc atctctccat tccggttaag gatatcgaat ccacgaaaaa attttatgtt
     3781 gaaggccttg gatgcggctt gggccgccag tcttccgttg ccatcacatt aaatttccac
     3841 ggccaccaaa ttgtcgccca tgtgaccgag gaccttggcc cccaacaaac aagtatctac
     3901 ccccgacatt tcgggctaat ctgcgggacg gaagaggagt ggaatgaact ccggaaccgg
     3961 gcccaaacga atagcctcat attttttcgt gaaccccgtc gacgttttcc tgatactcct
     4021 ctcgaacatt tgacgttttt cctggaagat ccgtcgcata atctgcttga attcaaatat
     4081 tacctcaatg cgaatgccat cttcggggaa gagacgtttt ctcaaattgg ggacgcccat
     4141 gaacagcatg ggtaagagtt tttccttcct gtgatcattt ttcagggtca cactatctga
     4201 gggcttgaag cacttcagta aaatgcccgt tcactttgac cttagggaat acgctcctta
     4261 tggtcccatc ttcattgatc actaccgtcg tacgttcaat ccccatatat ttccggccat
     4321 acattgactt ttctttccac accccatagg cctgaatcat ggccttagac tcatcgctta
     4381 acaagggaaa attgagttga aacttatcgg aaaacttccg gtgagaggag actgaatccg
     4441 cactcactcc gaacacgacc gccccgctct ttctcaattc ctgaatacca tcccgaaacg
     4501 aacaggcttc tgtcgtacat ccgggcgtgt catctttcgg gtagaaatag accaccactt
     4561 tttttcctcg aagctcagat gaattcacaa tgcgtccggt atcatcgggg agggaaaaag
     4621 ccggagcagg atccccaaac tgcaactcag acgtcgccgc cgtgcgtgga ctctgttgag
     4681 caggcttttt cgttttaggg gtagatgccg ttttgatttt tgttgatgga tttcgagacc
     4741 ctgcaggcaa cccctgactc tttgatgagg aacgcttggg tgctgccttc ttttttgcaa
     4801 cgaccgttgg ctttgattgt aacggtccct tcttcttagg ctgggctttt ttcactttgg
     4861 atgccatagg atcctcctct tatgactagt gggtaaaacg tgccgtgata aatcttttgc
     4921 tgaatgaaac tatcctgact caatacagga caatctcctc gtcgccctgg taatgacgga
     4981 ccattgtaca atgaataaga ggtttcattc ctcctttttt caaggatagg ggccacatgg
     5041 cccctatcct tgcggaggaa cggatcgcgt atgacattgg cgccaggcat caaccaagcc
     5101 atcgagccgg tcagtcagaa agcgctgacg ccatttgcag acgatttggg gtgtgatctg
     5161 cagagcacga gcgccatccg tattcgactg tccttcccac aggcaggata atccgggcag
     5221 gcgaggcaag cgtgttacat tttgaatggg cacaatcatc gagggatggg gcgtcctgtt
     5281 cgcatatgat ccgatttcgg tgaacgggca tttatacgaa ccacgaattt cggggtgaca
     5341 ctagtttagt ttagtttaat ttactgggcg ggtcagccgg aacacgca
//
LOCUS       DATIAL010000526         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1275665, whole genome shotgun sequence.
ACCESSION   DATIAL010000526 DATIAL010000000
VERSION     DATIAL010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275665"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VLA60_16225"
     CDS             complement(<1..331)
                     /locus_tag="VLA60_16225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246842.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HSF10958.1"
                     /translation="MKLEDQRTTVYKVDPNHPETESLPTLRTEELLLNMGPQHPSTHG
                     VLKVILELEGERIVKSTPVLGFLHRGVEKLAEGGTYHQFIPHTDRLDYVCAMYNNFAY
                     CRAVEKLM"
     gene            complement(385..879)
                     /locus_tag="VLA60_16230"
     CDS             complement(385..879)
                     /locus_tag="VLA60_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HSF10959.1"
                     /translation="MHPIAEKIQSRFPEGFVGANEWRGDLAITVKREALHDVCSYIRK
                     DPDLDCDYMVHVSSVDWPDDEERFEVVYEVYSIRKRHRIRIKTRVPEHDCLVDSMTDL
                     WMGADFMEREVFDMMGIRFNRHPDLRRILMPDDYTEGYPLRKDFPVQGKGWRDTFDFL
                     NDPT"
     gene            complement(899..1414)
                     /locus_tag="VLA60_16235"
     CDS             complement(899..1414)
                     /locus_tag="VLA60_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HSF10960.1"
                     /translation="MGLIQIGKPQKDGDLGIMTLSLEKAVNWARKGSLWPMTFGLACC
                     AIEMIAAVSSRYDIDRYGAGVFRASPRQSDLMIVAGTVCRRMAPVIRKIYDQMPEPKY
                     VISMGSCATSGNIYDSYSVVQGVDRFVPVDIYVPGCPPTPEALFDGILKLQDRIMQKR
                     VFTKQPEQVKV"
     gene            complement(1405..1788)
                     /locus_tag="VLA60_16240"
     CDS             complement(1405..1788)
                     /locus_tag="VLA60_16240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HSF10961.1"
                     /translation="MSGSDLLLEYLTKFLPILIFIGLAMGFGLVTLALSYLVQPKYPE
                     PEKLSTYECGSEPFSDSRMPFPIRYYVIAMLFVIFDIEVIFLYPWAITFNQLGMIGFI
                     EMMIFIGLFVVAYVYAWRKGALEWD"
BASE COUNT          819 a          698 c          668 g          729 t
ORIGIN      
        1 ccatgagctt ttccacagcc cgacaatacg cgaagttgtt atacatcgcg caaacataat
       61 ccagcctatc ggtatggggt ataaactgat gatacgttcc accctcagcc agcttttcga
      121 ctcctcgatg aagaaacccc agtaccgggg tggatttcac aatccgctca ccctcaagct
      181 ctagaatcac cttcaacacc ccatgcgtac tcggatgttg tggccccatg tttaaaagca
      241 gttcttccgt ccggagcgtg ggcaatgact ccgtttccgg gtggttcgga tcgactttat
      301 agacggttgt acgttgatct tccagtttca tgatctcggc ctagagcctg tcggctcacc
      361 ttgccccgat aagggactcg gcaatcacgt cgggtcgttt aaaaaatcaa aggtatcacg
      421 ccagcctttt ccctgaacgg ggaaatcttt gcgaagcgga tagccttcgg tgtaatcatc
      481 tggcatcaaa atgcggcgaa ggtccggatg gcggttaaac cggattccca tcatgtcaaa
      541 cacttcacgt tccatgaaat ccgctcccat ccaaagatcc gtcatggaat cgaccagaca
      601 atcgtgttca ggtacccggg ttttgattcg gatgcgatgg cgtttccgga ttgagtagac
      661 ttcataaact acttcaaatc gctcttcatc atcaggccaa tccactgaac tgacgtgtac
      721 catgtaatcg cagtccaggt cagggtcctt ccggatatat gagcagacat catgcagggc
      781 ctcccgcttg actgtaatgg ccaagtctcc ccgccactca ttggccccca caaacccttc
      841 aggaaatcgg ctttggattt tttccgcgat cggatgcatg gattcttcct gactttcctc
      901 agactttgac ctgttcaggt tgtttggtga acacccgttt ttgcatgatc cgatcctgta
      961 atttcaaaat cccatcaaac aatgcttccg gagtcggagg acaacccggc acataaatgt
     1021 caacgggaac gaaccgatcg accccctgaa caacgctata actatcataa atattgccgg
     1081 atgtagcgca tgaccccatg gaaatcacat atttcggttc aggcatttgg tcataaattt
     1141 tgcgaataac cggagccatt cggcgacaga ccgttccggc cacaatcatc aaatccgatt
     1201 gacggggcga ggcacggaac acgcctgcgc cataccggtc aatatcgtaa cgggatgaga
     1261 cggcggcgat catttcaatg gcgcagcagg ccaagccgaa cgtcatgggc cagagagagc
     1321 cttttcgggc ccagtttacg gctttctcca gggaaagagt cataatcccc agatcgccgt
     1381 ctttttgtgg cttcccgatt tgaattaatc ccattccaag gcccctttac gccaagcgta
     1441 cacatacgcc acgacaaata atccaatgaa tatcatcatt tcaataaacc cgatcatgcc
     1501 caactgatta aaggtaattg cccaaggata aaggaagatc acctcgatat caaaaatcac
     1561 aaacagcatc gcgatgacat aatatcgaat gggaaacggc atacgtgaat ccgaaaaggg
     1621 ttcagatcca cattcgtagg tcgaaagttt ttcgggttcg ggatatttag gttggaccag
     1681 ataggacaga gcaagagtaa ccagcccaaa ccccatggcc aggccgatga agatcaggat
     1741 aggaagaaat ttggtcagat actcaaggag aaggtctgag ccagacatcc ggggactccc
     1801 ctaaaaatag tttacaattc aaagccttct accgaggccg gcatcgtagc actctatttt
     1861 tttgtgaatc aatggaacga aagacctaac aggctaagga ccattcagac caacagggct
     1921 cgaaattaag attaatgtaa agttttcata agattgcagc ataccgtgcc ataaaacaga
     1981 gacaggtaat agtctggtaa aagtttttct catcactagg agtatacgga tatccggaag
     2041 tggaaaagaa taaattcccc acccccatca gagtggatgg cttgggaatc ctggcattcc
     2101 agtccatgtt ccgcaaatat agggtgttca cctgtttaat gcagaaaggc aaaggttacc
     2161 gtgaccttca tttgacactt gaggctctaa tgagtttagg gaacattcaa tcattatgtt
     2221 attaggcaaa tcaccttaaa tgcattctag aaatatgtta tctttcctgc tataaatcct
     2281 tttttaaatc aacgtccgct tccaattttc atcataaagt gaggaagaga aattagagcc
     2341 tgagcggaat aatctgaaat ttttgtgtgt gcccaatgtc ctcattaagg gaagtaaagg
     2401 tccctgaaga aacccacata caccggccaa tttgaagcga tgaaaggaat gacgtacgag
     2461 gaagaaattt gaggcggggt tttttctaac tcctaacctg aatcaaaact acgcacatgg
     2521 tcatagaagc ctgccctgcc tggcgatcac cacacaacaa gcgcacaacc tagggttttt
     2581 tcatctccga cgaaggagtc cgaagattag gaggggtatc tgattgatca ttccactcgt
     2641 cttccagggg agaaaccgct ttcgctttct ggcgacggtg aatttgagtt tggatccgtt
     2701 tttggggtga aggcgaggat tctccttctg gatcactttg gggtttttgt gagcgttcgg
     2761 agtgcataga aatagggtaa cacctggaga ccctaaaaag ctgtatttat cagaccatcg
     2821 ttcctcggga tttgaggggg gacttttgtg gaagaaaata aaatacggaa tgtggtcatt
     2881 gctgaaaagg gagtttttaa atgtgaaaaa aaga
//
LOCUS       DATIAL010000527         4579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_522881, whole genome shotgun sequence.
ACCESSION   DATIAL010000527 DATIAL010000000
VERSION     DATIAL010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522881"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="VLA60_16245"
     CDS             <1..957
                     /locus_tag="VLA60_16245"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HSF10962.1"
                     /translation="MITVDGHTVPDIGPLPTAVPVPATNLNYAAKLSLGKQLYFDGRL
                     SKNNAISCAFCHNPVAGFADPNQTSTGVGGKRGGRQSPTVYNTAFNTFQFWDGRAGSL
                     EEQAIGPIHNPVEMAETHEHVVPKIAKINGYKEEFQEVFGTGVSLQGIAEAIAAYERT
                     IISTNSAFDKFVLGDQQAMGEDAQRGMALFKGKGRCILCHNGSNFTDNRFHNLGVPQV
                     GPMEEDLGRYYVTLRERDKGAFKTPTLRSITESAPYMHDGAFKTLEEVIDFFDKGGNA
                     NPQLSPLMKPLGLAPQEKTDLIAFLKALTGEPIPFEFPKLPE"
     gene            complement(1405..2274)
                     /gene="dapF"
                     /locus_tag="VLA60_16250"
     CDS             complement(1405..2274)
                     /gene="dapF"
                     /locus_tag="VLA60_16250"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HSF10963.1"
                     /translation="MNNNFFKGHGLGNDYIALDPAKMSFKLTPRTIRALCDRHWGVGS
                     DGILALGPSKKADFGLRIYNPDGSEAEKSGNGLRIFGCYLYHTKHTRKKHFTVETKGG
                     LVEIHLELNGHGYVNGATVDMGRASFQPISLPCTLRVPELIQQPVKAAGQSLRFTGVS
                     VGNPHCVVYKKQEEQWTRQDLLEIGPELENHIIFPKRTNVQLAVPTGPRTISILIWER
                     GAGETQASGSSACAAACAGVRLGLVKSPVCVKAPGGTLDITVDPQYNITMKGPVTEVA
                     RGEISQAFIDGLR"
     gene            complement(2726..>4579)
                     /gene="typA"
                     /locus_tag="VLA60_16255"
     CDS             complement(2726..>4579)
                     /gene="typA"
                     /locus_tag="VLA60_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HSF10964.1"
                     /translation="KEPTVMLNQKRRKDLRNVAIIAHVDHGKTTLVDALLRQTGAHIF
                     KEGEAVIMDSNPLEKERGITIFSKNASLLYKDTRINLVDTPGHADFGSEVERILRMVD
                     GVLLLVDAFDGPMPQTKFVLKKSLELHLKPIVVINKIDRPSARPEEIVNQTFDLFCEL
                     NATDEQLDFPIVYASGKEGKSTLDMSDPAVDMKPLLDTIIHRVLPPVADPEQPFQMLV
                     TMLEYDSYIGRVAVGRIVHGMIEVGNQIVAVKRDGTISRGKVTKLLAYQGLTRVETDS
                     AQAGDIISLAGHQDVRVGETLASPINPTALPTVNVDEPTISMNFSHNTSPLAGKDGGR
                     FLTSRHIRERLAHEAMMNVGIKVEEADGGERFTVSGRGELHLSILIETMRREGFELEV
                     SPPKVIYKEMDGEILEPVELVVIEVDPGYQGAVIEALGGRKAEMKDLRISAVGTVRME
                     FHIASRALLGFRSELLRMTRGSGVMSQIFHEYQPFKGEIPHRSAGVLISHGPGQAVAY
                     GLWGLQDRGEIFLHPGDDIYEGMLVGVNNKGNDLVVNAIREKKLTNIRASGTDEAIHL
                     IPPREMTLEFALEFIEDDELVEVTPKNVRLRKRYLTDNERRSARNLSKKAQ"
BASE COUNT         1100 a         1115 c         1239 g         1125 t
ORIGIN      
        1 atgatcacgg ttgatggtca tacggttccc gacattggtc cactcccgac ggcggttcct
       61 gtgcccgcca ccaatttgaa ttatgccgcc aaactgtctc tgggtaaaca gctgtatttt
      121 gatgggcggt tatccaagaa caacgcgatc tcttgtgcct tttgtcataa cccagtggcg
      181 ggctttgccg accccaacca aacctcgacc ggggtgggtg gaaagcgtgg aggccggcaa
      241 tcaccgaccg tgtacaacac ggctttcaat acttttcaat tttgggatgg tcgagccggc
      301 tctttggaag aacaggccat tggtcctatt cataatcccg ttgaaatggc cgagacccat
      361 gaacatgtcg ttcccaaaat tgcgaagata aacggctata aggaagagtt ccaggaagta
      421 ttcggaaccg gggtgagttt gcagggaatc gccgaagcga tcgctgctta tgaacggacc
      481 atcatctcca ccaattcggc cttcgataag tttgtcttag gtgatcaaca agcaatggga
      541 gaagatgctc aacgggggat ggccctcttt aaaggaaaag gccgatgcat cctgtgtcac
      601 aacggctcta attttacgga taatcgattc cacaacctcg gcgtgccgca agtaggaccg
      661 atggaagaag atttggggcg gtattatgtg acccttcgag aacgcgataa aggagcattc
      721 aaaactccaa cgcttcgaag cattacggag tctgccccgt atatgcatga cggcgctttt
      781 aaaacattag aggaggtgat cgactttttt gataaaggcg ggaatgcaaa tcctcagttg
      841 agtccgttga tgaaaccatt gggattggcg ccgcaagaga aaaccgatct tattgccttt
      901 ctgaaagcat tgaccggcga accgattccg tttgagtttc cgaagttacc agagtaaacg
      961 ggcctaaaca ggagagtagg tgaatgctcg tttcgaggtt gtctcgcgaa ggtctgcgtc
     1021 tggttgtcgg gagaattagt gttggaggat gatcccacag gcaaagcaga gcggggcaaa
     1081 agtgtgtatg ggacctatgg ctattagctg aatggcaacg gtgcgaaagg tcagggtgat
     1141 aggtccggtg gcggatagtc tcaggtttag gcccgctgcc cttgcctggg agacggtgca
     1201 gaaaaagtct gagaaagaat tgttaatgat ttttcgagat ggaaagtcag ggacctggat
     1261 gcccttctgg aaaggacagt tatcgtagca gcatatacgg aatgtcttgg cctttctgtg
     1321 agagttctgg gggtaatccc agcaggttgc agaatcaaat ccgaaagcga gccgaggagt
     1381 ggggaataga tatttatttt tattttaacg caggccgtca atgaaggctt ggctgatttc
     1441 ccctctggcg acttcggtca cgggcccttt catcgtgatg ttgtactgag gatcaacggt
     1501 aatatccaag gttcctccag gggcttttac acacacgggg cttttcacta atcctaaccg
     1561 tactccggca caagccgccg cacaggcgga ggagccggag gcctgcgttt ctcccgcacc
     1621 ccgttcccaa atcaaaattg aaatggtccg aggcccggtc ggaacggcca gttggacatt
     1681 ggttcgtttc gggaaaatga tatggttttc cagttccggc ccgatttcaa gaaggtcctg
     1741 gcgtgtccat tgttcttcct gtttcttata caccacgcaa tgtgggtttc cgacactgac
     1801 cccggtaaac cggagagact gcccagccgc cttaacgggc tgttgaatca actcaggcac
     1861 ccgtaaggtg caggggagcg aaatgggttg aaacgaggct cgtcccatgt cgaccgtggc
     1921 cccattcacg tatccatgac cgttgagttc caaatggatc tcaaccaatc cgcccttggt
     1981 ttctacggta aagtgttttt tcctcgtatg cttggtgtga tacagatagc agccgaaaat
     2041 ccgcagtccg ttaccggatt tctcggcttc actcccgtcc gggttgtata tgcgcagtcc
     2101 aaagtcggct tttttcgaag gcccgagcgc cagaatgccg tcactcccca ctccccaatg
     2161 ccgatcacag agagcccgga tggtccgcgg tgttaattta aaagacattt tggccgggtc
     2221 gagagcaata tagtcattgc ccaagccatg tcctttgaag aaattgttgt tcatgcctgc
     2281 tccacaatgg gatggactgc tatcgggtga taggccttgc cggcaaggct ttttcccctc
     2341 tggacggtag gcctgaatta tacgtaaaaa tgatgaaaga ggaaagtact taagagggga
     2401 aaacgcctaa gggggcaaaa tcttatggtg agtagggcag agagttgccc gatagacact
     2461 tgagccggtc aatttgaaaa tgagaggggg cacccaggac tattctgcag cctttcgcga
     2521 ccaatgtcct gaagaattat tgagaaaggc ctgggaggaa ggtgtcctgc atcaattctg
     2581 gtcaaagggt attggagcca ggcgaacagt gatttgttcc acaaggcatg tgggttgatt
     2641 tggagagaat ggccatgggc ctcgccgcca cggttgagtg atggccaggc ccagaaatca
     2701 gatcaaaaat acaaggaggc aaaagtcatt gagccttctt ggacagattg cgagcggagc
     2761 ggcgctcgtt gtcggtgagg tagcgttttc gcaaacgcac atttttggga gtcacttcga
     2821 ccagttcatc atcttcgatg aattccaagg cgaattccag ggtcatttcc cgtgggggaa
     2881 tgaggtgaat ggcttcatcg gttccggagg ctcgaatatt agtgagtttt ttctcgcgaa
     2941 tggcattcac caccaggtca ttgcctttgt tgttgacccc aaccagcatg ccctcataaa
     3001 tatcgtctcc aggatggagg aaaatttctc cacgatcttg caggccccag aggccatagg
     3061 ccacggcctg tccaggacca tgagaaatga ggactccagc cgaacgatga ggaatctctc
     3121 ctttaaaggg ttggtattca tggaaaattt gggacatgac accgctgcct ctggtcatcc
     3181 tcaataattc actgcgaaat cccaaaaggg cacgcgacgc aatgtgaaat tccattcgga
     3241 cggtgccgac cgcactgatt cgcaggtcct tcatttcggc tttccggcca cccagcgctt
     3301 cgatgaccgc accctgataa ccggggtcaa cctcaatgac cacgagttcg acgggttcca
     3361 gaatttcgcc gtccatctct ttataaatga ctttgggcgg ggatacttct aattcaaatc
     3421 cttcccgacg catggtttcg attaaaatgg acaaatggag ttcgccgcgt cctgagaccg
     3481 taaatcgctc gccaccgtca gcctcctcca ctttaatgcc cacattcatc atggcttcgt
     3541 gagccaggcg ctcacgaatg tgtcgcgacg taagaaagcg ccccccgtct tttccagcca
     3601 aagggctggt gttatgcgag aagttcatgg aaatcgtggg ttcatccacg ttgacggtcg
     3661 gtaaggctgt gggatttata ggagaagcta aagtctctcc cacccgaacg tcctgatggc
     3721 cggccaggga aataatgtct ccggcttgag cggaatcggt ttcgacccgg gtgagcccct
     3781 gataggccaa cagtttggtg accttgccgc gggaaatggt gccatctcgt ttaacggcga
     3841 cgatctgatt gccgacttca atcatcccat ggacaatccg tcccaccgcc acacgaccga
     3901 tgtaggagtc atattccaac atagtcacga gcatttgaaa cggttgttcc ggatcagcca
     3961 cgggcggcag gacccggtga atgatcgtgt ccagtaaggg tttcatgtca acggcgggat
     4021 cactcatgtc cagcgtggac tttccttctt ttccggatgc gtacacaatg gggaaatcta
     4081 gctgttcgtc ggtggcgttc agttcacaaa acaaatcaaa ggtttgattc acgatttctt
     4141 caggacgggc gctggggcgg tcgattttgt taattacgac gatgggtttt agatgcagtt
     4201 ccaacgattt tttcagaacg aacttggttt ggggcatcgg gccatcaaag gcgtccacga
     4261 gtaagagcac gccgtccacc atgcgtagaa tgcgctcgac ttcactccca aagtcggcat
     4321 gccctggcgt atcgacgaga ttgatgcggg tatctttata gagaagcgaa gcatttttcg
     4381 aaaaaatggt aatcccacgc tccttttcca atggattcga atccataatg acggcttcgc
     4441 cttctttgaa tatatgcgcg ccggtctgcc tgagcagggc atccactaac gttgttttgc
     4501 catgatcgac gtgagcgatg atggcgacat tgcgaagatc ttttcggcgt ttttgattca
     4561 gcatgacagt cggttcctt
//
LOCUS       DATIAL010000528         3000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1383306, whole genome shotgun sequence.
ACCESSION   DATIAL010000528 DATIAL010000000
VERSION     DATIAL010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383306"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VLA60_16260"
     CDS             <1..575
                     /locus_tag="VLA60_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSF10965.1"
                     /translation="EHELNASTFAGRVTSSTLADLYGAVTAAIATLKGPLHGGANEAV
                     AEMFHAIRDPENAQEWVHAKLTGKERVMGFGHRVLKHEDPRSAIIKRRAKALSQHAAE
                     TRWYEIAEIIEQTMKEEKGLLPNLDFYTAVVYLFIGIPKEAFTPIFVASRLSGWCAHI
                     IEQQDHNRLIRPRASYIGPPAREFLFIGDR"
     gene            complement(756..1724)
                     /locus_tag="VLA60_16265"
     CDS             complement(756..1724)
                     /locus_tag="VLA60_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10966.1"
                     /translation="MDFPKFIEQIKKTYGITNDYALAKKLKVSQPEANWFRRGKKVPN
                     ATVCIRIANLLKKNPVELLIVAQKDRAAPEEKSHWAMALSAVDVMMHVPERPRYIPQK
                     VEAIGQELRQLESQMLSYEGTIAQNEAIQLLNTVESTIDSMMERWQIWKRDGPLFPSY
                     LLANQAAVQRGVKIRRVFIFNKEQMSDAAQIEDSLQVLNDQRHAGITAFFGFREQLAT
                     TLIYQRLVAEFRARGVKEEINAALFDNEILIFSRSYATRDLGMNSGSIPITVIDQLNI
                     SWKPDHLRDLNPSPLFETRFVFEYQGERQFKAQLGRFLRTYGKDKD"
     gene            2130..2861
                     /locus_tag="VLA60_16270"
     CDS             2130..2861
                     /locus_tag="VLA60_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HSF10967.1"
                     /translation="MQEFNAQVSRVKDLTHDVRELELSLLAPDAIDFKAGQWISLNVW
                     HPGLKQYVTRQYSIASPPNQCYKVNLLFNRIPDGLGSNYLFSFHEGDPVTFQGPNGSF
                     YLQEKPGRDLVFVATGTGIAPFCSMLSTFLEDPEAGTLTLYWGLRSQRDLYYQHELEA
                     LARRHPNFSFFTILSRPENGWKGSIGRVTTLVENNIASVSNVTFYLCGNGGMIRDVTA
                     IVRKKGLCPIRSEQYYDEAGAGTYA"
BASE COUNT          722 a          724 c          781 g          773 t
ORIGIN      
        1 ccgagcatga attgaatgcc tcgacgtttg ccggtcgggt cacgagctca accctggctg
       61 atctctatgg ggctgtcacg gcggctattg cgacactcaa aggccccctt catggcggag
      121 caaatgaagc tgtggcggag atgttccacg ccattcggga cccggaaaac gcgcaagagt
      181 gggtccatgc caaattgacc ggaaaggaac gggtcatggg gtttggccat cgagtcttaa
      241 aacacgagga tccgcgttcg gctatcatca aacgacgggc gaaagcgttg agtcaacatg
      301 cggccgagac acggtggtac gagattgcgg aaatcattga acagacgatg aaggaagaaa
      361 aggggctcct cccgaatctg gatttttata cggcagtggt ctacctgttc atcggaattc
      421 ccaaagaagc cttcacgccc atttttgtcg caagccggct gagtggatgg tgtgcccata
      481 tcatcgaaca gcaggatcac aatcgtctga tccggccacg agcctcctac atcggccctc
      541 ctgcgcggga atttctcttt atcggggacc gataagagac aagaaccgcc ccattactct
      601 gctgtcatca acggtaaagc gaagaaacta tgctgagacg tgtggcctca gtcatccttt
      661 tctgtatggg acttaggtcg caagaggatt cgaattttgg gctagagccc ttctgccgca
      721 ttccgcgcag ccggtgtgag cctggaagaa ataatttagt ccttgtcttt accgtatgtc
      781 cgaagaaacc gaccgagttg tgctttaaat tgccgttctc cttgatattc aaagacaaag
      841 cgggtttcaa agagagggct cggattcaga tcccgtaaat ggtccggttt ccaggaaata
      901 ttcaactggt caatgacggt gatagggatc gaaccactgt tcatgcccaa atccctggtg
      961 gcataggatc gggaaaagat gagaatttcg ttatcgaata acgcggcatt gatctcttct
     1021 ttgacgccac gggctcggaa ttctgcaacc aatcgttgat agatgagggt cgtagctagt
     1081 tgctcgcgaa atccaaaaaa cgcggtaatc cccgcgtgtc gttgatcgtt taatacctga
     1141 agggagtctt cgatttgagc ggcatcgctc atctgctctt tgttgaaaat aaacacccgg
     1201 cgaattttga ctccccgctg aaccgctgcc tgatttgcca ataaataact gggaaagagg
     1261 gggccatccc gtttccatat ctgccatcgt tccatcatcg aatcaatggt ggactccacg
     1321 gtattcagca attgaatggc ttcattctga gcaatggtgc cctcatagga aagcatctgg
     1381 gattcaagct ggcggagttc ctggcctata gcctccactt tttggggaat gtatctcggt
     1441 ctttccggca catgcatcat gacatcgacg gctgacagag ccatggccca atggctcttt
     1501 tcttccgggg cggcccggtc tttctgggcg acgattaaga gttcgacagg attttttttg
     1561 agaagattgg ctatccggat gcaaaccgtg gcattcggta cttttttgcc gcggcgaaac
     1621 cagtttgcct caggttggct cactttgagt tttttggcca gggcgtaatc attcgtgatg
     1681 ccataggttt ttttgatctg ctcgataaac ttaggaaagt ccatgttcgg ctcccaaaaa
     1741 atcaatccaa ttgaaccagt ggaatcaaaa aagatcattt tcactgccgg atgaggcgca
     1801 tgcgatccgg tcttgctaca gctaggtgat tctagacgaa ttcatccgga attaaaatgc
     1861 aggtatcttt ccggtagaaa ggtcaaaccg gcctggagtt ggccccgggc aatctggtga
     1921 ttctgtgatg tggggggaag tatttattag tcagggcgag tgacccacgc aatatcacag
     1981 aataagggga attggggcat cactttgctg tttctcagtg tgtgtttggt gcggcaatat
     2041 tccttaaatt tttgcaggcg gtttttcggc ttggtaatgt gtaatgtggc tctctagaga
     2101 gtcacctcaa tcggcaggaa tccaaaataa tgcaagaatt caatgcccag gtcagtcgag
     2161 tcaaggatct gacccacgac gtccgcgagt tggaacttag ccttctcgcc ccggatgcaa
     2221 tcgacttcaa ggcgggacag tggatctctc tgaacgtctg gcatccggga ttgaagcagt
     2281 acgtaactcg gcagtattcg attgcctccc cacccaatca gtgttacaag gtcaaccttc
     2341 tctttaaccg gattcccgat ggcctcggct caaactattt gtttagtttt catgaagggg
     2401 atcccgtaac atttcaaggg cccaatggat cgttttacct tcaagagaaa cccggacgag
     2461 accttgtctt tgtggccacg ggaaccggga ttgcaccttt ttgttcaatg ctttctacct
     2521 tcttggagga ccctgaagct ggaaccttga cgttgtattg ggggttgcgg agccagcggg
     2581 atctttatta ccaacatgaa ttggaagccc ttgcacggcg gcatccgaat ttcagttttt
     2641 tcacaatcct gtcccggcca gaaaacggtt ggaaaggttc catcggacga gtgacaacat
     2701 tagtggaaaa caatattgca tcagtgagca atgttacttt ttatctgtgc ggaaatggcg
     2761 gcatgattcg agacgtgacg gccattgtga gaaaaaaggg cctctgtccc attcgctctg
     2821 aacaatatta tgatgaagcc ggcgccggaa catatgcttg aatctgctcc gcgattcccg
     2881 atacaacgat caaccgaatg cttgtagagt cgcccaaggc atgcggcctc gcggaaggag
     2941 catttccgcg agcctcccgt cacttcccga tggcctcggc tcaaactatt tgtttagttt
//
LOCUS       DATIAL010000529         5319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1276554, whole genome shotgun sequence.
ACCESSION   DATIAL010000529 DATIAL010000000
VERSION     DATIAL010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276554"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VLA60_16275"
     CDS             <1..594
                     /locus_tag="VLA60_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HSF10968.1"
                     /translation="RNMIEEWSACLIQGHPEVISIHGRTLSQMYRGQSDWEAIALAAN
                     RIQPHGILVLGNGDIQTLGESAARIRESGVNGVLIGRGSLGNPWVFQGIQQIREMSQT
                     GQSCIPPQHVPSLEEKFSIMIQHASLFERVHGPERFVRMRKHLGWYCSGFPYAAAMRA
                     QLVRTHSTTDVINLVNQYQARALDNQHMEIPDTITAS"
     gene            595..1233
                     /locus_tag="VLA60_16280"
     CDS             595..1233
                     /locus_tag="VLA60_16280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HSF10969.1"
                     /translation="MPDPHLILASSSPRRKELLTLLGLSFEIISPAINEEVLGQESPA
                     NHVRRLALEKAESVASHYPDSLVIGSDTVIELDGQILGKPADLEDAHHMLTGLRGRCH
                     HVHTGLALIAKAKKLRETFVETVKVWVKDLLDADLQAYLKTQDSLGKAGAYSIQGEGA
                     HLIEKIEGDYPSVVGLPLSKIAQLLEQAGVELPVKAEAIYRTKPYANWKDFG"
     gene            1509..1868
                     /locus_tag="VLA60_16285"
     CDS             1509..1868
                     /locus_tag="VLA60_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10970.1"
                     /translation="MMTYFQQANLPLTLFQITIGKANPPDSIKHQRIETATQQQRQLT
                     EHERKMAEDQRLAAEISRAKADNAYREVTSLTPSQFIQLERINMQRTVCTQPTATCTF
                     IMDAALTPTLPVGKTNK"
     gene            complement(1959..2735)
                     /locus_tag="VLA60_16290"
     CDS             complement(1959..2735)
                     /locus_tag="VLA60_16290"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HSF10971.1"
                     /translation="MKFVCLKCETYMTFEKVEKPAEGSLGVFFECPSCQSRFSMVTNP
                     GETQMVSSLGVQLGGRTEASKPLEMTRGGLEDNVSAGMGQMAAYLNDKIQGGQSAAAS
                     APAASAAPASAGAPGTTSDGGGCPFSAMVAQMGLGSTGSTSAPAPVGEQILWTPDAQE
                     KLAKLPSFVQPMVKSSVETYARKNGFTTVTLQVMDDSKNASTEGIKWTPEAQQRLDNI
                     PDFIRPMARREIERLVKERGQSEITAQVMEEAKEKFMKFM"
     gene            complement(2785..3705)
                     /locus_tag="VLA60_16295"
     CDS             complement(2785..3705)
                     /locus_tag="VLA60_16295"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HSF10972.1"
                     /translation="MSQQNDLKTILNKLHYSDDAKVVEQISAQTKLVMARMKGIRRKV
                     VVMSGKGGVGKSMTTVNLALAFARMGQRVGLLDVDINGPCVPQMLGMRGKGLLDTPDG
                     AVPPTGPLNIKVASMDFLLQENAPVRWKGPMDLSPVWLGMTEMNVIREFLSDMVWGEL
                     DYLLADLPPGAAADKPPLLAGLIPDLAGAVVVTTPSEVASNVVQKSIAYAQELGIEIL
                     GVVENMSQYRCPSCGEESPLFEGDTDSMCEALGLPLLGRIPFDRNFARTFDKGEPILD
                     GDNPTAAKYQEIAKKVHTLLDYQHVLDGKV"
     gene            complement(3894..4187)
                     /locus_tag="VLA60_16300"
     CDS             complement(3894..4187)
                     /locus_tag="VLA60_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10973.1"
                     /translation="MTDFDREKLCETWQVHQSTDWPDGLGSHEGQLMTLDTVIGGCLT
                     YYFEEHHLDEPRIEILRDCLGDLEIIVPELSESTRDYFSRLRFLGVTLLQEYS"
     gene            complement(4252..4641)
                     /locus_tag="VLA60_16305"
     CDS             complement(4252..4641)
                     /locus_tag="VLA60_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10974.1"
                     /translation="MVGMFLFFIAGLWMADGVALLVAPERMIATLRQSLIVAPGFIKW
                     GGVAALLGLVLLFGTRGLPYQPLWMVVGLSMVAKGVFLYAGPDRWRLRIVKWCLEREV
                     VDYRIWGLGLCSLSVLLLDALGWGKGL"
     gene            complement(4733..>5319)
                     /locus_tag="VLA60_16310"
     CDS             complement(4733..>5319)
                     /locus_tag="VLA60_16310"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HSF10975.1"
                     /translation="RHGESQWNLENRFTGWVDVPLTPKGEQEARDAGKKLKAFRFDCA
                     FTSVLVRAKETLRLVLEEIGQTTIPIEENKALNERMYGELQGLNKTETAQKYGDHQVK
                     IWRRSFDVPPPGGESLKDTAERVLPYYENRIRPALLADKTVLVVAHGNSLRSLVMQLE
                     HLSKEAVLELNIPTGAPLWYELDASGEVTGHRYL"
BASE COUNT         1409 a         1512 c         1165 g         1233 t
ORIGIN      
        1 cggaacatga tcgaagaatg gagcgcatgc cttatccagg gacacccaga ggtcatctcc
       61 attcacggcc gcacgctatc gcaaatgtac cggggacaat ccgattggga agcgatcgcc
      121 ctagccgcaa accgaatcca gccgcatggc attcttgtcc tgggcaatgg cgatattcag
      181 acactgggag aatccgctgc ccgcattcgg gaatcaggcg taaacggcgt gttgattggc
      241 cgtggctcac tcggcaatcc atgggttttt caggggattc aacagatacg agaaatgtcg
      301 cagaccggac aatcctgtat tccgccgcag catgttccaa gcctggaaga aaaattcagt
      361 atcatgatcc aacatgcctc cctgtttgaa cgggtccatg ggccagagcg tttcgtacgc
      421 atgcgcaaac acttaggctg gtattgctcg ggctttccct atgctgcggc aatgcgcgca
      481 caactcgttc gtacccattc cacgacagat gtgatcaatt tggtcaacca gtaccaagct
      541 cgtgccctag acaaccagca catggaaata cctgacacca tcactgcctc ctaaatgccc
      601 gatcctcacc tcatcctggc ctcctcgtcg cctcgtcgaa aagaactgct aaccctcttg
      661 gggctttcct ttgaaatcat ttcgcccgca atcaatgaag aagttctcgg ccaggagtca
      721 cccgccaatc atgtacgacg ccttgctctt gaaaaagccg aatccgtggc gagtcactat
      781 cccgacagcc tcgtcatcgg cagcgatacc gttattgaat tggatggtca gatccttggc
      841 aaaccggcag acctagaaga tgcccaccac atgctgaccg gcttgcgcgg ccgatgccat
      901 cacgtccata ccggtttagc cttgatagca aaggctaaga agctaaggga aacatttgta
      961 gaaaccgtca aagtctgggt caaggacctt ttagatgccg acttgcaggc ctacctgaag
     1021 actcaggata gcctggggaa agcgggtgcc tattctattc aaggagaagg cgcgcacctc
     1081 atcgaaaaaa tcgaagggga ttatccttca gtggtggggt tgccgcttag caagatagct
     1141 caactccttg aacaggcggg agttgaactt cccgtgaagg cagaagccat ttaccgaaca
     1201 aaaccgtacg cgaattggaa ggattttggt taaaggattt gcagggtttc gcccccgctt
     1261 tctctgcaat aaacaaaaca aaaaaggtgg ctcttacaaa agagccgcct ttcaagaaaa
     1321 cagaatatta tttttggctt acataaactg cataaacttt taattggcct cctccatcac
     1381 ttcggccgtg atttccgact gacctagttc tttgaccaga cgttcgattt cccgccgtgc
     1441 cataggggtg gtgggatcgg gaataatatc taaccgttga cgcggtaaat cgggaagtca
     1501 ccggggaaat gatgacctat tttcagcagg ccaatttacc ccttacccta ttccaaatca
     1561 ctattggtaa ggcaaatcct ccagattcta ttaaacatca acggattgag accgccacac
     1621 aacaacaacg ccaactcacc gagcatgagc gtaaaatggc tgaagaccaa cgcctggccg
     1681 cagaaatcag ccgcgccaag gccgataatg cctatcgaga agtcacgtca cttacacctt
     1741 cccaattcat tcaactcgaa cgaatcaata tgcagcgaac cgtctgtaca caacccaccg
     1801 ccacctgtac tttcattatg gatgccgcac tcactccaac tctcccagtt ggaaaaacga
     1861 ataaataaag ccccataaag cagccagact cttaaaaaaa aaaccggcgg ctcctcttca
     1921 agagccgccg gttttaaaaa ggctatcgct tttgtagact acataaactt cataaatttt
     1981 tctttggcct cttccatcac ctgggccgta atttccgact gtccccgttc tttgaccagg
     2041 cgttcaattt cccgccgcgc cattgggcga ataaagtcgg gaatattatc caaccgttgt
     2101 tgcgcctccg gtgtccactt tatcccttcc gtagaggcgt tcttggaatc atccatcacc
     2161 tgaagggtca cggtggtaaa accattcttc cgggcatagg tctccacgct gcttttgacc
     2221 atcggctgca caaaagatgg cagtttggcc agtttttcct gagcatccgg cgtccataat
     2281 atttgttcgc caactggagc cggagcgctg gttgaaccag ttgagcccaa acccatttga
     2341 gcaaccatgg cggaaaaggg acatccgccg ccgtcagaag tggtgcctgg cgcaccagca
     2401 ctagccggag ctgcagaagc ggctggggct gaggctgctg ccgattggcc accttgaatt
     2461 ttatcgttca aataggcggc catctgtccc atacccgccg aaacgttgtc ctctagcccg
     2521 ccccgggtca tttccaaggg tttagacgct tcggttctcc cccccaactg gacgcctaaa
     2581 gaagagacca tctgggtttc gccgggattg gtcaccatcg aaaaccgcga ttggcaggag
     2641 gggcattcaa aaaatacccc aagagagcct tcagccggtt tttcgacttt ttcaaaagtc
     2701 atataggttt cgcatttgag acagacaaat ttcatattgc ttcccttctg aacgtcggcg
     2761 aaggccaacg atcgcttcga gacgttacac tttcccgtct aaaacatgtt gataatccaa
     2821 caatgtatga acttttttag cgatttcctg gtatttcgcc gcagtgggat tgtctccatc
     2881 caggatcggt tcccccttat caaaggttcg ggcaaaattc cgatcaaagg gaatacgtcc
     2941 taaaaggggc aaacccaatg cctcacacat ggaatcggta tccccctcaa acaaggggct
     3001 ttcttctccg caggagggac agcggtactg gctcatattc tcgaccaccc ccaaaatctc
     3061 aatacccagt tcctgggcat aggcaatgga tttttgaacg acattcgaag ccacctcgga
     3121 aggcgtcgtc accaccaccg cccccgctaa atcaggaata agtcccgcca agaggggtgg
     3181 cttatcggct gccgccccag ggggcaggtc ggccaacaaa tagtccaatt ccccccatac
     3241 catgtcggag agaaactccc gaatgacatt catttcggtc atgcccaacc aaaccgggct
     3301 gaggtccatg ggacctttcc accgcaccgg ggcgttttct tgtaggagaa aatccatcga
     3361 tgcgactttg atatttaatg gaccagttgg cggcaccgcc ccatccgggg tatccaataa
     3421 gccctttcca cgcatcccca gcatttgagg aacacaaggc ccattaatat ccacatccag
     3481 taatcccacc cgttgtccca ttcgcgcgaa agccaacgcc agattcacgg tggtcatact
     3541 tttccccacc ccgcccttac cgctcatcac taccaccttg cggcgaatac ctttcatacg
     3601 ggccatgacc agtttggtct gggccgaaat ctgctccacc accttggcgt cgtcagaata
     3661 atggagttta ttcagaatcg ttttcaggtc attttgttga ctcattgtct gcacctaact
     3721 gtgaaaggca ataggtagaa accagaaacc acggtaccac agcatttttt ccagcgtcaa
     3781 accaatcaaa aaaccaactc catgaaatcc agtaaggctg aatagcccat aaagagcctc
     3841 atttcggtgg gcaacggaaa atgaccagga agaaggatcc gtttgaaaat ggtttaggaa
     3901 tattcttgaa gaagggtcac gccaagaaat cgcaatcgac tgaaataatc acgggtggat
     3961 tcagataatt ccgggacaat aatttccaga tccccgaggc aatcacgtaa gatttcaatt
     4021 cgtggttcat caaggtgatg ctcctcaaag taataggtca aacatccacc aatgactgta
     4081 tctaacgtca tgagttgccc ttcatgactc cccaacccgt ctggccaatc ggtagattga
     4141 tgaacttgcc aggtttcgca caatttctca cgatcaaaat ccgtcatatg ccctcattat
     4201 tatcgtactt ttggcatagc tggatcagat agtcttcccc attccaccca gctacagtcc
     4261 ttttccccag cccaacgcat ctaatagcag gacagaaagg gaacataacc ccagccccca
     4321 gatgcgataa tcgaccacct cccgttccaa acaccatttg acaatccgca accgccaacg
     4381 atctggtccg gcatataaga aaacgccctt ggcgaccata ctcaggccca cgaccatcca
     4441 aagaggttgg tagggcagtc ctctggttcc aaataacaaa acaaggccca ataaggctgc
     4501 aacgccaccc cattttataa accctggcgc cacaatcagc gactgtcgca gagtggcaat
     4561 catccgttcc ggcgcaacta acaaggccac accatcggcc atccacaacc ctgcaataaa
     4621 gaaaaggaac atgccgacca tgattcaacc aagcaggaat aaggcaattt tctctgcgaa
     4681 aagattcttt aaaatttcgc tttggagaca ttggcaatgc gctatggctc actcacaaat
     4741 accgatgacc ggttacctcc cctgatgcgt ccaattcata ccataatggt gcacccgtcg
     4801 gaatattcaa ttcaagcaca gcttccttgg acaaatgctc taattgcata accaaagacc
     4861 gcaaactatt gccatgggcc acgactaaaa ccgtcttatc tgccaataga gccggccgga
     4921 ttcgattttc ataataggga agaactcgtt cggcagtatc cttcaagctt tcacccccgg
     4981 ggggaggcac atcaaagctc ctccgccaaa ttttgacctg gtggtcgcca tatttctgtg
     5041 ccgtctctgt tttattaagc ccttgcagtt ctccatacat ccgttcattc agcgctttgt
     5101 tttcctcgat gggaatcgtc gtctgtccaa tttcctccag gaccaatcgt agggtttcct
     5161 tggcacgcac caacactgag gtaaatgcac aatcaaatcg aaacgcctta agtttcttac
     5221 cggcatcgcg ggcctcttgc tcccccttcg gagttaatgg gacatcgacc cagcccgtaa
     5281 aacggttttc cagattccac tgagattcac catgacgaa
//
LOCUS       DATIAL010000530         8438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1276904, whole genome shotgun sequence.
ACCESSION   DATIAL010000530 DATIAL010000000
VERSION     DATIAL010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276904"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VLA60_16315"
     CDS             complement(<1..778)
                     /locus_tag="VLA60_16315"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HSF10976.1"
                     /translation="MAIKRIPTTSAQVGMYLCGIDRSWIKTPFLLHRFLIKSSSDIAK
                     LKQAGIQEITIDTDRGRDVVSDSEPPHAPDDVSSPPNSPIPDSSRPPAVTRLDQLSPS
                     VQGKSLSGELTTMKMVRMYMLEGIQDILHTLQKTGRLAMEQVHQITESVLAESLRHEE
                     ACIAMILTRDFSPALFDHALAVSTLAVLLGRAVGLDNTALQHLASAGLLHDAGLLNIP
                     QILHRPLNQFSDAELVLYHKHPHRGVEMLSQQSSLSDEVDT"
     gene            1101..1895
                     /locus_tag="VLA60_16320"
     CDS             1101..1895
                     /locus_tag="VLA60_16320"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HSF10977.1"
                     /translation="MGGCRASFHGGFSDSISVGPGTQRDVVSTSWSFLICGLFLVFLS
                     WGFPSLGHAASESSPPIKHVRTVIPILGTTMNAQSEQVGIVAEIQLEFLQRRDHKGLD
                     LQFLSRPGKFSPYAQQSVKDALGLVVEAAGLNPDSWTVKFILPYPGVTLYGESLSAMA
                     ALNVVALAKNEPVDEETVLTGTVTADGHVGTVGGVPLKILAAYEQRYRRVLIPEEPDV
                     ADDDWETPFLMEVTPVGSIKHAYLALTHRPLRSHLNDQSFAASLTH"
     gene            2109..2777
                     /locus_tag="VLA60_16325"
     CDS             2109..2777
                     /locus_tag="VLA60_16325"
                     /inference="COORDINATES: protein motif:HMM:NF018627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10978.1"
                     /translation="MIRPCFAVDIDNVLGRAEPEVQRLFQELTGRLWPVGLYGSAGGL
                     DASQLAPELLEEIFSRFHQESIPRLPLFPGAKQALTLIHRRYRVIIVTARRPYSRPQT
                     LRWLERHGLPFDALYHTEEKTDIPETITAAIDDHPHHIQGYRALDRQVFVMDQPWNRA
                     IPHADVIRVSGWDALVQWLHVRRLPSWTKHIPESPTFRQLFSVHLNVSSLTAAGVSTG
                     FAQP"
     gene            2910..3395
                     /locus_tag="VLA60_16330"
     CDS             2910..3395
                     /locus_tag="VLA60_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF10979.1"
                     /translation="MMTLPIHTVLLVDDNPDDCEIVKEAWDEIRVGKELRCVNDGTEL
                     LHYLYCCGPFSRRECAPRPSVILLDLNMPHMTGGEVLTEIKKDSCLASIPIVVLTTSK
                     APRDICHTAGMGVNGYIQKPNSYTGYLRMFTNLKKHWGEILAQPLAGSGGDTSTNVAW
                     C"
     gene            3839..4690
                     /locus_tag="VLA60_16335"
     CDS             3839..4690
                     /locus_tag="VLA60_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10980.1"
                     /translation="MKTHSILTGAVIMMGTTLLTTSFAYEASEYLLLTRHRDSNGHLL
                     AGPTHHQSQPSVIQKTVVPAQPQTFERRSTEWGMNAQEVKLNEPVQPVWELQSPVLEP
                     YEQRVGYRRQIEGIDASLTYTFYEDQLAQANYLFEPKHDDAVDYVKDFHTLQNWITQS
                     YGMPTLVQEIWLNSLYQYDQSLWGQALLRGHLKMVAEWRNDTTNITLLLDGGEDTIGL
                     MADFDSVAVIAPASMEAKVIEEIPPVEETTIEKVSVSEEHAMEAPSGEEMPTEAISQE
                     NQPDTKL"
     gene            complement(4914..5525)
                     /locus_tag="VLA60_16340"
     CDS             complement(4914..5525)
                     /locus_tag="VLA60_16340"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSF10981.1"
                     /translation="MTYLRAVILSPFIFLYKLVFLVFLTVLCVTGCSTPYSSTLPPDL
                     AEQIDPLLLFPRIKEDPASFKGKLIILGGQVLAAKRLKDATQLTILQLPLSNDQEPTT
                     ELTQSQGRFIAEQEQFLDPATVPPGSRITLTGELAGSVQQSLDETIYTYPTLIIKHFK
                     VWPAYPPDYARYGPYPQPLFYPYGFPYWGGYGRFYPYYPFGYW"
     gene            complement(6000..6323)
                     /locus_tag="VLA60_16345"
     CDS             complement(6000..6323)
                     /locus_tag="VLA60_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10982.1"
                     /translation="MKYFSILAVLCSMFLATPVWAHGDASHVMGTVTELGNDHVVVIT
                     PKGESLTLAFHPQITFQQNGIHVEQARPQVGDRLVAEVSKKGVPENRDWVATEISFST
                     PKKKP"
     gene            complement(6349..6954)
                     /locus_tag="VLA60_16350"
     CDS             complement(6349..6954)
                     /locus_tag="VLA60_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF10983.1"
                     /translation="MLRRIKILFIIILLLLVGSTLAALWLLPGTFSAKATPPEWEVKA
                     ARFIRHLATPSHFLKMSNPVPFSPENLAQARHHFADHCATCHANDGSGKTHMGPNFYP
                     PVPDLRDPAIQAMADGELFYIIHYGIRFTGMPAWGKGLPEEDLDSWKLVQLIRHFPDI
                     TPEEITEMKQYNPLTREEREQQEALDQFLSGEDVFPQEHHH"
     gene            7155..>8438
                     /locus_tag="VLA60_16355"
     CDS             7155..>8438
                     /locus_tag="VLA60_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF10984.1"
                     /translation="MTSSQLVASLQDPKCYSHPVSQVTVVETHISWVLLTGQYVYKIK
                     KPVLFSFVDFSTLEKRRWFCEEEVRLNRRLAPQLYLGVVSITGSPSDPRMDGDGSPFE
                     WAVKMKQFLPDQEFHHLLETGNLDESVVGQLGIDIGTFHGRIESAGDPLSYGEADRVW
                     GPIAECFEDIPLAALPARIQFCMKDIEEWTNQEWKRLRPTLRERKSEGQIRECHGDLH
                     LGNIALFEGRICVFDALEFKPGLRWIDVISEVAFLVMDLESRDRVDLAYWFLNCYLEV
                     TGDYAGMSVFRLYEVYRALVRAKVAGLRLAQVEPGGPVREDILKEIIRYVELAHRLMR
                     PAPPMLMLMHGVSGTGKTTVSTEVVKALGAMRVRSDVERKRIHAEQRKIVVDQGTSKS
                     LYGSDMTRVTYDRLLDLANTMLQAGYSVIVDATFLK"
BASE COUNT         2038 a         1805 c         2270 g         2325 t
ORIGIN      
        1 aggtatcaac ctcgtccgaa agactcgact gttgggacag catctcaacc ccccgatggg
       61 gatgtttatg gtataggact aattctgcgt cagaaaactg gttgagtggt cggtgaagga
      121 tttgtggaat gttcaagagc cctgcgtcat ggagaaggcc cgcgcttgcc aggtgttgta
      181 atgctgtatt gtccaaaccc acagctcgtc ccaataaaac tgctaaagta ctgaccgcga
      241 gtgcgtggtc gaataaggcc ggagaaaagt ctctggtcag aatcattgcg atgcaggctt
      301 cttcatgtcg gagggattca gccaagacgg attccgtgat ctgatgaacc tgttccatgg
      361 cgagccgtcc ggttttctgc agtgtgtgga ggatatcctg gatgccttca agcatataca
      421 tccgcaccat cttcatcgta gtgagttcac cggatagtga ttttccctgc acagagggtg
      481 aaagctggtc aagccgtgtg actgcgggcg ggcgggagga atcaggtata ggtgaattcg
      541 ggggggatga gacgtcatca ggggcgtgtg gcggttcaga atctgacact acgtcacgcc
      601 cacgatcagt atcaatggtg atttcttgaa tgccggcctg ttttaatttg gcaatatcgc
      661 tggaagattt gatgagaaac cggtgcagta ggaatggggt ttttatccat gaacggtcga
      721 ttccacagag gtacattccc acttgagcgg aagtcgttgg aattcgtttg atggccatat
      781 gtataccgat gaggggttct taggaaggaa atttcatttc ttcattgtgg ggaatgcctg
      841 gtgtcctcat cggcgtcctt tcggccgttc ttaacctcat cctcagtagt gaacacgtgg
      901 tgagagtgaa gggcttgtta tgtctaagga tgtgtttgtg atagggggga tgcttctgtt
      961 gtgcaatgac agaaaatttt gtgaattgat aaggcccagg gaccatcaga gccagcaaaa
     1021 tatagtgcct gacctcatga aatttaagtg ttctccttgc caggccgaaa aggagaagac
     1081 agctttcatg ggagatggcg atgggtggat gtcgagcatc atttcacggg ggtttttcag
     1141 attccatctc tgttgggcct ggcactcaac gagatgtcgt ttcgacatct tggtctttcc
     1201 tcatatgtgg cctcttcctc gtgtttttgt catggggatt tccttccctc ggccatgctg
     1261 cttctgaatc gagtccacca atcaaacatg ttcgaaccgt gattcccatt cttgggacca
     1321 cgatgaatgc acaatccgaa caggttggca ttgttgcgga aattcaattg gaatttcttc
     1381 agagacggga tcacaaaggt ttggatcttc aatttctttc caggccaggg aagttttcac
     1441 cctatgccca gcaatcggtc aaagacgctt taggtctggt ggttgaagca gccgggctga
     1501 accctgattc atggacggtc aagtttattt tgccctatcc gggagtcacg ttgtacgggg
     1561 aaagtttatc ggctatggcg gccctgaatg tcgttgctct tgctaaaaat gagcccgtgg
     1621 atgaggagac ggttttgacg ggcaccgtca cggcggatgg acatgtggga actgtgggag
     1681 gagtcccgtt aaagattttg gccgcgtatg aacaacggta tcgacgagtg cttattccag
     1741 aggaaccgga cgtcgcggat gatgattggg aaaccccgtt tctgatggaa gtgacaccgg
     1801 tgggatccat caaacacgcc tatctcgcgt tgacccatcg gcctcttcga agccatctta
     1861 atgaccagtc ttttgctgcc agcctgaccc actaacttca ctcctattga acattccgtc
     1921 ctgtgtatag tgatctctgt gagggaggag atttcccatg tgtggtggat gaaaggggtt
     1981 ttgcctccgc ttgcttgttt ggttcgaata tttctatggt agaaaaaagg gaaaggaaca
     2041 acttctggct tgggcctttc tcttttgcag gtttttgtgt tgaaggaaat atctaggggt
     2101 aattttttat gatccgacca tgttttgcgg ttgatattga taatgtctta ggccgggcgg
     2161 agcctgaagt gcagcgttta tttcaggaat tgacgggaag gttatggcct gttggtctgt
     2221 atggcagtgc cggtggatta gatgctagcc aattagcgcc tgagctcctt gaagagattt
     2281 tctcccggtt ccaccaggag tctatcccac ggctaccttt gtttcctgga gcaaaacagg
     2341 ccttgaccct catccatcgt cgctatcggg ttatcatcgt gacagcccgg cgtccgtatt
     2401 cacgacctca gactcttcgt tggttagaaa gacatggtct tccttttgat gcgctgtacc
     2461 acactgagga gaaaaccgac attcccgaaa ccataacagc tgcgattgac gatcatcctc
     2521 accatattca gggctatcgt gccctcgata gacaagtttt tgtcatggat caaccctgga
     2581 accgtgcaat tcctcatgct gatgttattc gggtgagtgg ttgggatgct ctggtgcagt
     2641 ggttgcatgt ccggcgtctc cctagttgga ccaaacatat tccggagtct cctacttttc
     2701 gacagttgtt ctcagttcat ctcaatgttt cttctttgac cgcggccggt gtttcgacgg
     2761 gattcgcaca gccgtaagcg tgcgagggtt gggggacagg aaagttttta aaggaaattg
     2821 ccgataaagg tcagaggttc tttgctgttg agaagcggta tgacccggca cagattttga
     2881 tctcctgttt agctaattcc agaaaactga tgatgactct gcctatccac acggtcctgc
     2941 ttgttgacga taatcccgat gactgtgaga ttgtcaaaga ggcatgggac gagatccggg
     3001 taggaaagga attgcggtgt gtgaatgatg gcaccgaatt gctgcattat ttgtattgct
     3061 gtggtccatt ttcgaggaga gaatgcgcgc cacgtcccag tgtgattctc ctggatttga
     3121 atatgcctca catgaccgga ggggaggttc tcaccgaaat caagaaggat tcatgtttag
     3181 catctatacc cattgtggtg ttgactacgt ctaaggctcc aagggatatc tgtcataccg
     3241 ccgggatggg ggtaaatgga tacatacaaa aacccaattc ttataccggg tatctccgaa
     3301 tgtttactaa tttgaaaaaa cattgggggg aaattcttgc acaaccattg gctggaagtg
     3361 gcggggatac ctctaccaat gttgcctggt gttaaaagtc ggtgccgtgc ggtatttgcc
     3421 attcttccag gcaaattttg gtgcctgttc tttcctctct cttcgtcccc tgcgactgct
     3481 cctgctctag attggtgttt cacctccagt atggctcggt aggcgaaatg ccttgaactg
     3541 aatactcaat tagatttctt cataactaac cgattcctcc tttttttcgt aacaggcatt
     3601 tttctcttcc agtggtcctg cttttcaaca tttcacatcc taggccggga tggtgaaggt
     3661 tcgctctgtc cggataagcg gcttctgctc ccccctcaag ttctctcctc aacaaccgac
     3721 actatcctat attgactaac gagtagtgat aacgggagat ccacctgtgt attcatcctt
     3781 tcagggatcc tctgatcatt ccttgcgatt gaaaaccttg ggggcaatgg gaaatgctat
     3841 gaaaacacat tctattttga cgggcgcggt tatcatgatg ggaacaacac tgttgacgac
     3901 aagttttgcc tatgaggcct cagaatattt actgttgacc cgtcaccggg attcgaatgg
     3961 tcatttgctg gcgggaccaa cccaccatca atcgcaacct tccgtgattc aaaaaacggt
     4021 cgttcctgcc caacctcaga cgtttgaacg gcgaagcacg gagtggggaa tgaatgccca
     4081 ggaggtaaag ctgaatgaac cggtgcaacc ggtttgggaa ctccaatctc cggtattgga
     4141 gccctacgag cagcgggtag ggtatcgtcg tcaaatcgaa ggaattgacg cgtcgttaac
     4201 ctatacgttt tatgaagatc aacttgctca ggcaaattat ttatttgaac ccaaacatga
     4261 tgatgcggtg gactatgtga aagattttca tactttgcaa aattggataa cccagtccta
     4321 tggtatgccg acattggttc aggaaatctg gttaaattcc ttgtaccaat atgatcaaag
     4381 tctctgggga caagccctgc ttcgtggcca tctaaagatg gtggcggaat ggcggaacga
     4441 taccacgaat attacgctcc tgttagacgg aggagaggat acgatagggt taatggctga
     4501 tttcgatagc gtggcagtca tcgcccctgc ctcaatggaa gccaaggtaa ttgaggaaat
     4561 tcctcctgtg gaagagacca caattgaaaa agtctctgtc agcgaagagc atgcaatgga
     4621 agccccgtca ggggaagaga tgccaactga agccatatcc caggaaaatc aaccggacac
     4681 aaagttgtaa atttaccgct catttttcag ctaagttgta atactcgtgt attgtaggag
     4741 gaggccatgc ctcctctttt ttttattcta atattttcaa atggtgaatc gggttcccgt
     4801 ggcatagtgg agttcttggg cggttccaac ctttagaagt gatggagtgc ttgaatacct
     4861 gggcctcttt ggaaaatcag tggagagaga gtctgtggtt tggggagaga gtcttaccaa
     4921 tatccaaaag ggtaataggg ataaaaccgg ccatatcctc cccagtacgg aaacccatag
     4981 ggatagaaca agggttgagg ataaggtcca taccgggcgt aatcaggtgg ataggctggc
     5041 catactttga aatgtttaat gataagagtg ggataggtat atatggtttc atcgagactc
     5101 tgttggacag agccggccag ttctccggtc agggtgatcc ggcttccagg agggactgtg
     5161 gcgggatcta gaaattgttc ctgttccgca ataaatcgtc cttgggattg cgtaagttcg
     5221 gttgtgggtt cctggtcatt gctgaggggt aattgaagga tcgtgagttg ggttgcatct
     5281 tttaagcgtt ttgccgccag gacctgccct ccaagtatga tcagttttcc cttgaagctg
     5341 gcaggatcct ctttgatgcg tgggaacagt aatagggggt cgatttgttc ggccagatct
     5401 ggaggaaggg tgctactgta gggggtggag catccggtga cacaaagcac ggtaaggaat
     5461 acaagaaaaa ccagtttata gagaaaaata aaaggtgaga gaataacggc ccgcaagtaa
     5521 gtcatcttgc tccttcatca tctttttaca gcatattcct ccaccgtgaa gagatgcaaa
     5581 tacaacagat tcatctgatg attctgttga cccgagagtc ttctttggta gcgggactgg
     5641 ctggatttat cccggatggg acctcagtcc tcccgtttaa gtgattatac actccggccc
     5701 atttgctcaa ttttggctat ttgtcctgct tgtttatgag gtcggtcgtc agggagttct
     5761 gaggcatcgc tatgaacccg ggatccagtc atgggcatga cgttttcatg gacctttaaa
     5821 ggcaggaaaa atgattaaaa tcctgggggg atcgaaaaag gcaattatct taaataagga
     5881 ggatacggga agaattaagt ttttgttcat tccatcagcg tggaagcatg gatttggctt
     5941 tccctaaaaa atcgattgac ttgtgtttct catgcttggt cgtgagtcgt attcaggaat
     6001 caaggcttct ttttaggcgt ggaaaacgag atttcggtcg caacccagtc tcggttttca
     6061 gggacgccct tttttgaaac ctcggccacc agtcggtctc caacctgcgg ccgggcctgc
     6121 tcgacatgaa tgccattttg ttgaaatgta atttgaggat gaaaggcgag agtcagggat
     6181 tccccttttg gagttatgac caccacatga tcatttccca attcggtgac ggttcccatt
     6241 acatgagagg catcgccatg ggcccagacg ggggtggcca gaaacatgga gcacaaaacg
     6301 gccagaatgc tgaaatattt catgatggtg actccttgga ttataaaatc aatgatggtg
     6361 ctcttggggg aagacatctt ctcctgataa aaattgatca agggcttctt gttgctcccg
     6421 ttcttcgcgg gtaagaggat tatattgttt catctccgtg atttcttcag gagtgatatc
     6481 ggggaaatgt cgaataagct gtactaattt ccaactgtcg agatcctctt ccggcagacc
     6541 tttcccccag gccggcattc cggtaaagcg aatgccgtaa tggatgatat aaaacaattc
     6601 tccatcagcc atagcttgaa tcgcagggtc cctgagatca ggaacaggag gatagaaatt
     6661 cggacccata tgggttttcc cacttccatc attggcgtgg caggtggcgc agtgatcggc
     6721 aaaatgatgc cgagcttgtg caagattttc aggcgaaaaa gggacggggt ttgacatttt
     6781 gagaaagtgg ctgggggttg ccaggtggcg aataaacctg gcagctttca cttcccactc
     6841 aggcggcgtg gctttggcag agaacgtccc cggtagtaac cagagcgcag ccaatgtgga
     6901 cccgaccaac aggagaagaa tgataataaa aaggattttg attcgtctca acataattgg
     6961 tcagttattt atagtgtacc tgttcgggga ttttcttcac aagtcaaatg aacgttcatc
     7021 atcagtggat gcttttccct ggttggggaa agaagggaaa ttgggtagta tgggcaaatg
     7081 cattcagtga ttccggtccg aaacgatgta attttatctc ctctcatgtg gccgatggcc
     7141 tttatttgat ggctatgact tcttctcagc tagtcgcatc gttgcaggac ccgaaatgtt
     7201 attcacatcc tgtgagtcag gttacagtgg tggaaacgca tatttcctgg gtgttgctaa
     7261 ccggtcagta tgtctacaaa attaaaaagc cggtgttgtt ttcgtttgtt gatttttcga
     7321 cacttgaaaa acggagatgg ttttgtgaag aagaggttcg tctcaacaga cggttagccc
     7381 cacagctcta tctgggggtg gtctccatca ccgggtcccc atcagatcct cgcatggatg
     7441 gcgacggaag cccttttgaa tgggccgtca aaatgaaaca atttcttccc gatcaggagt
     7501 ttcaccatct ccttgagacg ggcaacctgg acgaatctgt ggtcggtcaa ctgggcatag
     7561 atattggaac attccatggc cggattgaat ctgcaggaga tcccctttcc tatggagagg
     7621 cggatagggt atggggacct atagctgagt gctttgagga catacctctg gcagccctcc
     7681 ctgcgcggat tcaattctgt atgaaggata tcgaggaatg gacaaaccag gaatggaaac
     7741 gattgaggcc aacactcaga gaacgcaaat ctgaagggca gattcgagaa tgtcatggtg
     7801 accttcattt aggaaatatt gccctatttg agggccggat ctgtgtgttt gatgccttgg
     7861 aatttaaacc cgggttgcgt tggattgatg tcatcagtga agtcgcgttt ttggtcatgg
     7921 atttagagag cagggatcga gtggatttgg cctattggtt cttaaactgt tatctggaag
     7981 tgactggtga ctatgccggg atgagtgtgt ttcggctgta tgaggtttat cgggcacttg
     8041 ttcgagcgaa agtggctggc ttgcgtctgg ctcaggtcga accaggaggc ccagtccggg
     8101 aggatatcct taaagaaata atcagatacg tggagttggc ccatcgattg atgaggcctg
     8161 ctcctcccat gttgatgctg atgcatggag tatccgggac tgggaagacg acggtctcca
     8221 ctgaagtggt gaaagctttg ggtgccatgc gcgtacgatc cgatgtggaa cggaaacgga
     8281 ttcatgccga acaacgtaaa atcgtcgttg atcagggaac ctcaaagagc ttgtacgggt
     8341 cagacatgac tcgtgtaacc tatgatcggt tattggatct tgcaaatacg atgttgcagg
     8401 cgggctattc cgtcattgtg gatgctacat tccttaag
//
LOCUS       DATIAL010000531         5861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_632419, whole genome shotgun sequence.
ACCESSION   DATIAL010000531 DATIAL010000000
VERSION     DATIAL010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632419"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VLA60_16360"
     CDS             complement(<1..1175)
                     /locus_tag="VLA60_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10985.1"
                     /translation="MGEMTRPDFSRAMFGFFILQIGLLSWAGWVNQDWISTDGISYIR
                     IAQYYASGQFDLAVSGYWGPMLSWIIVPLLGFMEESLSAAQMAMALSAIVYLVGCMAI
                     FRVLGIGSSAVLVALGIVAFFSAHWAVQGITPDLLMSGILCLAISRTLHPDWCDKPSV
                     QFSAGLLFGAAYLAKAPGLPFAFILIVAAGALWTVCQQTSPIHALRSAAITFFGLMLI
                     AGPWILVLSLKYERLTFSTSAGISHAVMGPAGIERGHPTFSDFLIPEQGRLSSWEDPI
                     SKKYSHWSPLESTENFRHQLRLVHSNVMKTIRLLKDFDWIGIGLVSVILGFLFHAPWR
                     QNIRSDRWRWAAILIGCLVVIYLPGYGGERRYYYSVYPFLLGGSLGFVGYLAKSRSG"
     gene            complement(1733..2722)
                     /locus_tag="VLA60_16365"
     CDS             complement(1733..2722)
                     /locus_tag="VLA60_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HSF10986.1"
                     /translation="MLSTQSSNYFRVLWPYVQIARIDHWFKNVFMILGIVLALFYEPE
                     LFAWASLPPLLLAVAATCLIASSNYVVNELLDAPYDLLHPVKKDRPVPSGKVKSSWAW
                     MEWFLLGVSGMVLAFSLNAYFGTTALLFLISGLIYNIPPIRTKELPYLDVLSEALNNP
                     IRLLLGWFALITHSIPPLSLVLAYWMVGAFFMATKRYAEYRRIGDPIRAQGYRKSFRY
                     YTENRLLLSMFFYAMACSFVSGIFLVRYHVELILFIPIGAGFLTYYLKIGLLEDSPVQ
                     NPEKLYKERGFVLYVAFSTLFFLLLMFSEIPDLYELFNVNPALIPPLWTIGAK"
     gene            complement(2700..3428)
                     /locus_tag="VLA60_16370"
     CDS             complement(2700..3428)
                     /locus_tag="VLA60_16370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HSF10987.1"
                     /translation="MKGVHQIRAVLLDIDGTLYHQQSLRALMAFELSTAPFALGSMRE
                     ALRVWRILKCFREGREALRTMEDPSAPLVSLQYSLVAERVCERPSEVEIVVREWMFRR
                     PLKYLRICRRRAMKEFFQLAADRGLRTGVFSDYPVREKLQGLGIHAPLSVELCATDPE
                     INAFKPNPKGFLYACHLWNFDPWEVLYVGDRYDVDACGAKAAGMPYVIFSRRAPRTLD
                     ASAKTEYRSITNFRGLCDVIHSII"
     gene            4724..>5861
                     /locus_tag="VLA60_16375"
     CDS             4724..>5861
                     /locus_tag="VLA60_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HSF10988.1"
                     /translation="MIAAISLEAPSLPLIQQLIADNRMPHLAALQREGEVVSLDPPFL
                     DGAVYATLYTGQHLSDHGIYSLFTWSAPEQRLRLSHELLSDDTLFRRLDRAGKRVLAI
                     DPPEHPIQELANGIVVSGCQFRSRVHLAQWSRPPSLGKELSKYLGKTPRGEETFGQPS
                     QDHLLHLRKILLQAPSRLASGVERLMEGRAPDVLWLHMAAIHLAGHQLWDTTSVDVIG
                     QSSTGTSLLEHSLIDVYLAADAALGRIIKFLPDDADLLVFWAKGMGPETCRSDLLPEM
                     LERILTTPSPVHTSSGNDILTKLRAIVPTSFRSKVADALPDSLSLQLTARLGTMGKDW
                     KAVRAFSLPSDGPGLVRLNIRGRERSGIVSESEAKVLCDEIAEGL"
BASE COUNT         1652 a         1537 c         1333 g         1339 t
ORIGIN      
        1 tgaccggatc gactcttggc cagatacccc acaaagccta aactcccgcc aagaagaaac
       61 gggtaaacgg aataataata tcgcctttct cctccataac ccggcaaata gatcaccaca
      121 agacacccga tcaatatcgc tgcccatcgc cagcgatcag accggatatt ctgtctccat
      181 ggggcatgaa acaagaatcc caatatgacc gaaaccagcc cgattccaat ccagtcaaag
      241 tccttcagca gacgtatagt tttcatcaca ttactgtgaa cgagccgcaa ttgatgacga
      301 aaattctctg tactttcaag cggggaccag tgtgagtact ttttcgagat tggatcctcc
      361 caggaggaaa gtcggccctg ttccggaata agaaagtcag aaaaagtggg atgtccccgt
      421 tcaataccag ctggacccat gacggcatga ctaattcctg cacttgtcga aaacgtcagc
      481 ctttcatatt tgagggacag taccaaaatc caaggcccgg caataagcat taacccgaaa
      541 aaggtaatcg cggcgctacg tagagcatgt atcgggctgg tctgctgaca aacggtccat
      601 aacgcccctg ccgccacaat cagaataaat gcaaatggca acccaggcgc tttcgctaaa
      661 taggcagctc cgaataatag cccggcagaa aattggacgg atggcttatc gcaccaatct
      721 ggatgcaaag tccggcttat ggctagacaa agaatgccac tcatcaggag gtccggagtg
      781 atcccttgaa ccgcccagtg ggcactgaaa aaggcaacga tcccaagagc gacaaggacc
      841 gcggaggacc cgatccccaa aacccggaag atggccatac agcccacaag gtacacaatc
      901 gccgacagtg ccatggccat ctgcgcagca gatagcgatt cttccataaa gccaagcaac
      961 ggaacaatga tccagctcaa catgggcccc caataaccgg aaaccgctag atcgaactgt
     1021 ccggatgcat aatattgggc aattcgaata taagaaatcc catcggtact gatccaatcc
     1081 tgattgaccc acccggccca actcagcagc ccgatctgaa gaataaagaa gccgaacatg
     1141 gctctggaga aatctggccg agtcatctca cccattagac ataacctttt tcagaaagat
     1201 gccttgaaat aaagcggttc ccaaatcgga gcttcctctt ctttcaaata agataagtaa
     1261 tcttccactt tggaatttca tcatttttat ttccccctcc cacttattca taatcagaac
     1321 tccggatgcc tccgaacgcc gggaagacga tccatcacga aagggctcaa cgttcgaagt
     1381 ccgtgaattc ctcatgcttg aaacccaaca ttcaaaaccc agccacaatt tcgtcagata
     1441 ttagggggca gagtcgtcct agcgggcggc aatcctccac aatggtgaag agagcttctc
     1501 atccgtccca gaatatcttt ccagggaggc agtcccctct ttaacgtctt caatattccc
     1561 caacccatgg cgtggtcatg caggtcacaa agttttgttt aattcctcgg gattcctgct
     1621 taagactgaa ggaatctttg gtggttacct tgtccatcgc ctaacgaaaa tggacacaca
     1681 aaccaaagcc tcgggtattc ttgaatcatt tctctaggca gatgacgctc acttacttag
     1741 caccaatagt ccaaagaggt ggaatgaggg ccggattaac attaaataac tcatacaaat
     1801 cagggatctc ggaaaacata agcaggagaa agaacaacgt gctgaatgcg acatacagaa
     1861 caaacccgcg ttccttgtat aatttttctg gattttgaac gggactatcc tcaagcaagc
     1921 caatcttaag ataatacgtc aaaaatcccg cccctatagg gataaaaaga atcaactcca
     1981 catgatatcg gacaagaaaa attccagata caaaggaaca ggccatcgca taaaagaaca
     2041 tactgagcaa taggcgattt tcggtgtaat accggaacga ctttcgatac ccttgtgcac
     2101 gtatcggatc acctatgcgc cgatactcgg catatcgctt ggttgccata aaaaatgccc
     2161 caaccatcca gtacgccaac acaagcgaaa gtggagggat gctatgggtt ataagggcaa
     2221 accacccgag caacaaacga atcggattat tcagtgcctc agaaagcaca tctaaataag
     2281 gtagttcttt agtccgtatc gggggaatat tgtaaatgag gccgcttatt aaaaataaga
     2341 gggctgtggt cccaaaatag gcattcaggg aaaaagcaag aaccatccct gaaacgccca
     2401 ataggaacca ctccatccag gcccaactgc ttttaacctt cccggaagga acgggccgat
     2461 cctttttcac tggatggaga aggtcataag gcgcatcaag aagctcgttc acgacataat
     2521 tgcttgaggc aatcaaacag gtggctgcaa cagccagaag caaaggagga agactagccc
     2581 aggcaaaaag ttccggctca taaaacaggg ccagcacgat gcccagaatc ataaagacat
     2641 ttttaaacca atggtcgatc cgggcgatct ggacataagg ccagagcacc cgaaaatagt
     2701 tagatgattg agtggataac atcgcaaaga cctctaaaat tagtaatcga acgatattct
     2761 gtttttgcac tcgcgtccaa cgtgcgaggc gctctgcggg aaaatatgac gtaaggcatt
     2821 ccggcagctt tggcgccaca cgcatcgacg tcataccgat caccgacata caacacctcc
     2881 cagggatcaa aattccagag gtgacaggca tagagaaatc ccttggggtt aggtttaaag
     2941 gcattaattt ccggatccgt ggcgcataat tccaccgata acggagcatg aatccctagc
     3001 ccttgcaatt tttctcttac aggataatca gaaaaaactc ccgtacgcag acccctatca
     3061 gccgctaact gaaaaaattc tttcatggca cgacgccgac agatcctgag atatttcaag
     3121 gggcgacgga acatccattc cctcaccacg atttccacct cactgggtct ctcacaaact
     3181 cgttcggcaa caagggaata ttgcaaactc accaatgggg cactgggatc ttccatggta
     3241 cgtaacgctt cacgtccttc ccgaaaacat tttaaaattc tccagacacg caaagcctcc
     3301 cgcatagaac ctaacgcaaa cggagcagta gacaactcaa aggccatcag ggcacgaaga
     3361 gactgctggt gatagagcgt cccatcaata tcgagtaaaa cggcacgaat ctggtgaact
     3421 cctttcacga cacagtctac ccctcaaaat cgggaaaatg gctattcaaa aattggctct
     3481 caaacgctcg tggatacgat atggactgtg caaatacgta tattcatcag attattgaat
     3541 gattgttcaa aaataccctt catgtgctct caagaattcc ttgagccata aaagcaagaa
     3601 ttgttccgta ttgaatctat tcggaatcca gaattacggg ctgttaaaaa agtttaaaaa
     3661 acgaaccagt gggagtccgt atccgatagc ggccgtttag attacgcgca ggtctcattt
     3721 agccaaggtg gagaactcta agcggggcct atgttctcgc ttggctcttc ctgtgaaaca
     3781 ccctgttttc tggcgaccgt aatggtgatg gtgatagttg ggactacttt gtcctacaag
     3841 gccatcgtcc actattagag ggagaccgct ttttttacca actgccggcg gggaaagacg
     3901 attgatcccc ataggatccc taggttgtag atggttgtca gcgttttttt gcgtttttgc
     3961 cttttttctc tcctcctcta agcagctgaa aggattatgg aaactatagc ctttggcatt
     4021 ttttagggga atgacgaggg ctatgtggat aatgatacat gattcctcgt aatccacgct
     4081 gcaatcgatc actttcagga aggggaagtt agattatggt ccattactct aacggctggc
     4141 tgggcaactc ggttgggtgt catgaggctt gaagatatca tcgcgtcgat cattacaacg
     4201 aagagtttac caaggacacc actcatatcc atggcattga atggggggag tttcgccaat
     4261 gtgcgcctgg caaaataccg tgcatggact tgatcgccgc tatacttaca cttgtatccg
     4321 gaggaatgca actggcgcta caacccatga ccggccaacc ttgataaacg cttgggcaag
     4381 accttcatct aaggtcctcc tagccatagc ctagatttaa tgggcacaat ggcaacccag
     4441 gttaaccttg atacagaata cctgggattc aattggtcta ttgaaaggtg taactcgata
     4501 acaccaatag attgatttta ctgactcaaa cctggcatct tcaaaatttg aagaacattc
     4561 aaaatggcta aaccttacaa ggattatctc caaaacaatc aatgattttt gaggcgccga
     4621 attccctgga catgccctcc cctgttcgct ttcgacgtag cacccgcagg tgccaggacc
     4681 tcggtttcat ctgtctaatc tgctctgtca tagggagcaa aacatgattg cggccatctc
     4741 tttggaggcc ccatcgctgc ccctcatcca gcagttaatc gctgacaacc gaatgccgca
     4801 tctcgccgct cttcaacgag aaggcgaggt cgtcagcctc gaccccccat ttcttgatgg
     4861 agcggtgtac gccaccctct ataccggtca gcatctgtcg gaccacggga tttactcctt
     4921 gtttacctgg tctgctcccg agcaacggct ccgattgtct cacgaacttc tgtcagatga
     4981 tactctgttc cgacgcctgg accgggcagg taaacgagtc cttgccatcg atccacccga
     5041 acatcccatc caagagctgg ccaatggtat tgtggtgagt gggtgccagt ttcgatcacg
     5101 cgtccacttg gctcagtggt cgcgtcctcc gagcttgggc aaggaacttt cgaaatatct
     5161 tggcaagaca cctagagggg aggagacctt tggtcagcca tcgcaggacc atctcctcca
     5221 cctgcgaaaa atcctcctgc aagcgcccag ccgcctagct agcggggttg aacgattgat
     5281 ggaagggagg gcccccgatg tcctctggtt gcacatggct gcgatccacc tagcgggtca
     5341 ccagctttgg gatacaacgt ccgtcgacgt catcgggcag tcatcaacgg gaacgagtct
     5401 gctggaacac tccttaattg acgtatatct tgccgccgac gcagcacttg gtcgaataat
     5461 caaatttctg cctgacgacg cagatctcct cgtgttctgg gccaagggaa tgggcccgga
     5521 gacatgtcgg tccgatcttc ttcccgaaat gttagaacgg attctgacca ctccctcccc
     5581 ggtccacacg tcatcaggga atgacatttt gacgaagctg cgtgcgattg tacccacctc
     5641 gtttcgctct aaagtcgcag atgctttacc ggattcactt tcgctccaac tcacagctcg
     5701 actggggacg atgggaaaag actggaaagc ggtacgggcc ttttcgctac cttcagacgg
     5761 ccctggattg gttcggctca acatccgcgg gcgtgagcgg agtgggattg tctccgaatc
     5821 cgaagcaaaa gtactctgcg acgagatcgc cgagggtctc c
//
LOCUS       DATIAL010000532         4334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_955242, whole genome shotgun sequence.
ACCESSION   DATIAL010000532 DATIAL010000000
VERSION     DATIAL010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955242"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..950)
                     /locus_tag="VLA60_16380"
     CDS             complement(81..950)
                     /locus_tag="VLA60_16380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HSF10989.1"
                     /translation="MVENFFIDTESLAQNLGREDLVIIDVRGRGAYTSHIPGAVHSTW
                     HEYSDPSAVAKGILDPDLGRIEQRLRVLGINKSSDVVIYSNPFDNWGDEGRMFWMLQY
                     LGHPSIRILDGGWVKWIAEQRPFEHEAVKPKPGDFTITADPELIINKEGLKKLVKGSH
                     PETILIDARSVEEYAGKEIDGLPRAGHIPSAINIPWNRFLQPDASVKPPGQVRKIFED
                     HGLREDQEIITYCLGGIRAAWMYCLLRYVGYPRVKTYPGSWWEWSRDFAAPAEKDVKL
                     LYKVTNQDSVKPS"
     gene            complement(963..2285)
                     /locus_tag="VLA60_16385"
     CDS             complement(963..2285)
                     /locus_tag="VLA60_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10990.1"
                     /translation="MKQKRASRRKIWMFLILALGVAIYIFYTEIRPAVIFGLRDDYAK
                     PIPYQQIPVGLQSLKAEECGRCHVEIYQEWKSSIHAHAFKDPFFQAYWKKDNNIWVCL
                     NCHTPLENQQPTLIQDLPRGRVEKAYEIPNYRYDPDFQQEGVTCAACHVRDGVILGPY
                     DDSAAPHPTQFDPSFRTTQVCYRCHNVVSGPTQFYNAGPCGTYPEYEGKFFMKERGLI
                     CQSCHMPEVERPVAKDSPIRYGRRHLWRGGHDPEMIKRAVAVQVQTVPPSPQPGDDVT
                     FTLTLINAGAGHKIPTGDPDRYFTVEFTVRDPNGTVVHQQSDTMGRWILWQPVIVEVY
                     DNRLLPLASRDYTFEYEIPASEKGWRVQARIRYHILTDAQHRMLKEEYHLTGEDPYVF
                     TIYEREFPLDATLSVALEDQQADPRIGCTAPSDGLSPHPTAKSPSLHS"
     gene            complement(2290..3234)
                     /locus_tag="VLA60_16390"
     CDS             complement(2290..3234)
                     /locus_tag="VLA60_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HSF10991.1"
                     /translation="MGRVGYVSHPAYLLHDMGRSHPESPERLRAIRARLESSGTWARL
                     KQVVPRRAERKWIELVHRSSYVDSLEQRSPSEGYVSLDPDTTMSPETLNAAYLAAGGA
                     LTAVDAIMNHEIDQAFCAVRPPGHHAEANRAMGFCFFNTVAIAARYIQRHHGLKKVLI
                     VDWDVHHGNGTQHAFYDDPTVMFFSTHQYPHYPGTGGDTETGAGKGKGLTINVPLSGG
                     QGDAEYRDIFQNILVPNAAIFQPECIVISAGFDAHRDDPLAGMALTERGYADLTTIVA
                     EIANTYAGGKIISCLEGGYHLQALAGSVEQHLLALLES"
     gene            3451..3771
                     /locus_tag="VLA60_16395"
     CDS             3451..3771
                     /locus_tag="VLA60_16395"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF10992.1"
                     /translation="MANPKIEQFKKVLQMDPKDETLWFGLGKAYMSDENWEEAITALE
                     ECIRVKPQYSAAYLALAQSLHQKHEFEKCRSICNEGITAATKNGDLLVIKNLEELKTA
                     LPSP"
     gene            complement(3808..>4334)
                     /locus_tag="VLA60_16400"
     CDS             complement(3808..>4334)
                     /locus_tag="VLA60_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF10993.1"
                     /translation="ECYEGTHRFFVEVTVIQSTRGSLRKPRKVGQPHQIPESSDEFFE
                     QALVKRLLSRMAEKAKQLERYCAPVLLAVSIPDLPREKGRSHEIPPLDLQRLAAMLVG
                     VLVEIPQFSAVLLTFWKAAAQESRNPIRIREVSWITRPVGDSRDPRIRLLATNPGARY
                     RLTTRELRIVQEAM"
BASE COUNT         1109 a         1164 c         1019 g         1042 t
ORIGIN      
        1 cattcctgac ctcctctgaa aaatgtgaat gatccttgaa cttgggggat tggatatcct
       61 ctcacgcact actaccaaga tcaagatggt ttcaccgaat cctgattagt gactttgtat
      121 aacaacttaa cgtctttttc cgctggagcc gcgaaatccc gagaccattc ccaccaagaa
      181 cccgggtagg ttttcactcg cggatagccc acgtaccgta acaaacaata catccaggcg
      241 gcccggatgc cgcccaggca ataggtaatg atttcctgat cctcccgaag accatgatct
      301 tcaaatattt ttctgacctg accgggaggt tttacgctgg catcaggttg taaaaatcga
      361 ttccagggaa tattgatagc agaaggaata tggccggcac gaggcaaccc gtcaatttct
      421 ttcccggcat actcctccac acttctcgca tcaataagaa tagtttccgg gtgagatcct
      481 ttgaccagtt ttttcagtcc ttctttattg ataatgagct ccggatctgc cgtgatcgta
      541 aaatcaccag gcttgggttt gactgcttca tgttcaaacg gacgctgctc cgcgatccac
      601 ttgacccatc caccatccag aatgcggata cttggatgtc ctaaatactg caacatccag
      661 aacatacgtc cttcatcacc ccaattatca aaagggttgg aatatatgac cacatcgctg
      721 gacttattga ttcccaagac ccgaaggcgc tgttcaatcc gccccaggtc cggatccaga
      781 attcctttcg ccacggcaga aggatcgcta tattcatgcc aggtgctatg gacggcaccg
      841 ggaatatgcg aggtgtatgc cccgcgtccc cggacatcaa taatgactaa gtcttcgcgg
      901 cccaggttct gggccagtga ttcggtatca ataaaaaaat tttcgaccac ggttcgttcc
      961 ctttatgaat ggagtgaagg cgattttgcc gttggatggg gagaaagccc atcggacggt
     1021 gccgtgcagc caatccgagg atcggcctgt tgatcctcta aggcgacaga cagggtcgcg
     1081 tccaagggaa actcccgttc atagatagta aacacgtaag gatcctcacc cgtcagatga
     1141 tactcctcct tcaacatccg gtgttgcgca tcggtgagga tatgataccg aattcgtgcc
     1201 tgaaccctcc accctttttc acttgcagga atttcatatt caaaagtgta gtcccgactg
     1261 gccagcggca gcagtcgatt atcgtatact tccacaataa caggctgcca gaggatccat
     1321 cgacccatcg tatcggattg ctgatggact acggttccat tcgggtcccg aacagtaaat
     1381 tcgactgtaa agtaccgatc gggatccccg gtcggaattt tgtggcccgc cccggcattg
     1441 atgagcgtca gcgtgaaggt cacatcgtct cctggttggg gtgagggagg aacggtctga
     1501 acttgcacag ccactgcgcg cttgatcatc tctggatcat gcccgcctcg ccagagatga
     1561 cgccgaccat accggatggg actatccttg gctaccggtc gttcgacttc cggcatatga
     1621 caactctgac aaatcagtcc cctttccttc atgaaaaact tgccctcata ctccggatac
     1681 gttccgcaag gccctgcatt gtaaaattgc gtgggtccgg acacgacatt atgacagcga
     1741 taacacactt gagtggtacg gaaggatgga tcaaattggg tgggatgagg cgccgccgaa
     1801 tcgtcatacg ggccgagaat caccccatcc cggacatggc aggccgcaca ggtcacgcct
     1861 tcttgttgaa aatccggatc ataccgatag ttgggaattt catatgcttt ttccacccgc
     1921 ccgcggggaa gatcctgaat aagggtgggt tgctggtttt ccaatggtgt atggcaattc
     1981 aaacagaccc agatattatt atcttttttc caataggctt gaaaaaatgg atctttaaag
     2041 gcgtgtgcat gaatgctgga tttccattcc tggtaaattt caacatgaca ccttccacat
     2101 tcttccgctt ttagactctg caatcccacc gggatctgtt gatagggaat gggtttcgcg
     2161 tagtcatcgc gtaacccgaa aatgacggca gggcgaatct cggtataaaa aatataaatg
     2221 gctaccccaa gcgccaggat gagaaacatc catatttttc ttcggcttgc tcgtttctgt
     2281 ttcatcctgt caactttcca gaagggccag aagatgttgc tcgacagatc ccgccaaagc
     2341 ctgaagatgg tacccccctt ctaaacacga gataattttt cctcctgcat aggtattggc
     2401 gatttcggcg acaatggtcg tcaaatcggc ataccctcgc tccgttaacg ccatacccgc
     2461 taaaggatcg tctcgatgcg catcgaaccc ggcggaaatg acaatacact cgggttgaaa
     2521 tattgcggcg tttggaacca ggatattttg gaaaatatcc cgatactcag catccccctg
     2581 tcctcctgac aacggcacgt taatggtcag gcctttccct ttgcctgctc cggtctccgt
     2641 gtcccctccc gtcccgggat aatgaggata ctgatgcgtg ctaaaaaaca tgaccgtcgg
     2701 atcgtcatag aatgcgtgtt gcgtgccatt gccatgatgc acatcccaat ctactatcag
     2761 caccttcttc aatccatggt gtcgttgtat ataccgagcg gcaatggcca cagtattgaa
     2821 aaagcaaaaa cccatcgccc gattcgcctc ggcgtgatgc cccggaggcc gcacagcgca
     2881 aaacgcctga tcaatttcat gattcataat ggcatccact gccgtcaggg ctccaccagc
     2941 cgccagatag gctgcattca gcgtttcggg agacatcgtc gtatcgggat ccagcgaaac
     3001 atagccctct gaaggagagc gctgttcaag gctatccaca taagaggagc ggtgtacgag
     3061 ttcaatccat tttcgctccg cccgacgggg tacaacctgc ttcaggcgag cccatgttcc
     3121 agaactctct aagcgtgcgc gaatcgctcg aagccgctcg ggggactccg gatgactccg
     3181 gcccatatca tggagtaaat aagccggatg agaaacgtaa cctacacgtc ccataatcaa
     3241 acacccacaa accgtaaaag gagatcatgc acagaggaga aattcgatta ccaatcaacc
     3301 acccgatatg ctccgcatat acggacaagc tggaagggaa aacgtaagag atcctaccat
     3361 agggtggtag cttagacaac gcgtggacct gcatgatagg ctcatttctg ttcagtgttc
     3421 atcacatctc atctgatagg atttcaccca atggccaatc ccaagattga acagtttaaa
     3481 aaagttttgc aaatggaccc gaaggatgaa accttgtggt tcggcttggg aaaagcctat
     3541 atgagcgacg aaaattggga agaagcaatt acggccttgg aggaatgcat cagggtgaaa
     3601 cctcaatatt cagccgctta tctggctctg gctcaatcac ttcaccaaaa acacgaattt
     3661 gagaagtgcc ggtccatttg caacgaaggc atcacagcag cgacgaagaa tggcgattta
     3721 ctcgtcatca aaaatctgga agaacttaaa actgccctgc cttcccccta atcccccttg
     3781 ctcctccgca ctcatccatc ggttcattca catggcttcc tggacaatcc ggagttccct
     3841 ggttgtgagg cgatagcgtg cgcccggatt tgtggccaat aatcggattc gaggatcgcg
     3901 cgaatctcca accggccgag tgatccagga aacctcgcga atacgaatgg gattgcggga
     3961 ctcctgagcc gcggccttcc agaatgtaag caataccgca ctgaattgag ggatttcgac
     4021 taacaccccg actaacatgg ccgctaaccg ttgaagatcg agcgggggaa tctcatggga
     4081 acgacctttc tcccgcggta agtcggggat actcactgcc agcaacacgg gtgcgcaata
     4141 tcgttctaac tgcttggctt tttctgccat tcgtgacaac aaccgcttca ccaatgcctg
     4201 ctcaaaaaat tcatcggaag attctggaat ctgatgaggc tgtcctacct tcctgggttt
     4261 tcgaagagat cctcgggtcg attgaataac cgtcacttct acaaaaaaac gatgtgtgcc
     4321 ttcataacat tcta
//
LOCUS       DATIAL010000533         3805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_955452, whole genome shotgun sequence.
ACCESSION   DATIAL010000533 DATIAL010000000
VERSION     DATIAL010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955452"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..1657
                     /locus_tag="VLA60_16405"
     CDS             377..1657
                     /locus_tag="VLA60_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF10994.1"
                     /translation="MNSSNQLDRKERELEAARKISQALFQHLSVEDVVEQGLKIALEV
                     VNCRAGSVLLANPETKELVFYHSIGDQPLKPGTAFPWTQGIAGSVFATGEPVVIKDVK
                     EDQRHLEKIDQLSGFHTRDLIAIPLNRWEGHPIGVLEVMNKREDRLNQDDVAILMIIG
                     DFTALAIEQARLFQEAKLAEVARILGDIGHDVKNMLMPVLCGTSLLRDEMNDVFADLP
                     AFDPDKGRASHELCLEVIGMVANNAKRIQERVKEIADCVKGLTSPPRFAPCKLHHVIA
                     SVFDTLKLAAQEKGITLAHEGILELPVIQADESRLFNAFYNLVNNAISEVPSGGSITI
                     KGQMEAMGKTVHVSVIDTGRGMPAEIRDTLFTARAISRKQGGTGLGTKIVKDVIEAHS
                     GVIAVESEVNRGTVFHIHLPIEMSTTFSSDQVSS"
     gene            1816..3786
                     /locus_tag="VLA60_16410"
     CDS             1816..3786
                     /locus_tag="VLA60_16410"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HSF10995.1"
                     /translation="MGHSPLRSSAFQIGVITLMVFLGVMGLRWEGFLESAELDAYDWS
                     MRLRPTNTRPPPPITLVSITDQDIRTLGHWPVTDEVLARALDVMTAFHPRAIGVDIYR
                     DLEVPPGRQELDRVLEAHPEILMVMKFGKIEKGGIPGPAMLQGTDRLGFNDVVVDSGG
                     IVRRGLLFLDDGTNFYRSFSLLLALQYLQQEKIVPKPAVENSDWLQLGETVIRPFEAH
                     DGGYVEADAQGYQYLLNLERGKNVFPTISLGDILEGNFNPELLQDHIVLVGVIAQGVK
                     DYFYTSQCGTLTVCPPIPGLELHGYMVHQLLRLAKGEGQAPIATFPDSLETAWIGLWV
                     LGGGFIGVRVRGAWRFSLAVLMGLLLLSGVVVSGMMYGTWIPFFPAVIGLVVNAMVVT
                     AWLSNREKQDRALLMSLFSRHVSSEVAQAVWEKRDQFLEKGRLRPQKLVVTTLFTDLE
                     GFTTVAENMEPDTVLDWLNTYMERMVKIITNHGGMVDDYHGDMIKADFGVFRLEQTEA
                     NKRQDVNNAVSCAVALEQEMRHLNTQWQQQGLPEVRMRIGMQSGPVVVGSLGSEQRLK
                     FTTIGDSVNVASRLESFQKDSLETWSKDEVCRILIGETTKHYLGDHPWRLKEVGVLTL
                     RGKANRISVYRLFTNTGSPEPRSAISIEQAGG"
BASE COUNT          912 a          886 c         1081 g          926 t
ORIGIN      
        1 gtctgtattc ttgtgtacgc tgaattgtac gaagcatggc aatactctct tattcaggct
       61 cttgcgccaa atcggtagga ttgacgtcac ccgggatcct atgcaaacgc aaaccgatcc
      121 agcgccaatc tgtttcaagc gtcaacgacg agagcctgca ttctttagtc ttattcaatt
      181 cggactccgc gagcgcagga gccagtccgg gaaggttcga atgacaccag gtacataccg
      241 cgtcctcttg tgtgatccct ttaaggccca ataagctttt ttattcaaat tttgctaccc
      301 ttttcgaata actctccaaa agtgaatcgg ctatggtgaa aattgggtca cccttgacat
      361 cattgcggag ccggatgtga attcctcaaa ccaattggat cgcaaagagc gggaactgga
      421 agccgcccgg aaaattagcc aggcgctttt tcagcatctc agcgtggagg atgtggtgga
      481 acagggatta aagatagcct tggaggttgt gaactgccga gccggttccg tgttgttggc
      541 caatccagaa acgaaggaat tggtgtttta tcattccatt ggtgaccagc cactcaaacc
      601 cggaacggca tttccatgga cgcaaggcat tgcgggatcg gttttcgcca caggagaacc
      661 tgtggtgata aaagatgtga aggaagatca gcgtcatctt gagaaaatcg atcaactgag
      721 cggatttcac accagagacc tgattgccat tcctctgaac cgatgggaag gacatccgat
      781 aggtgtcctg gaggtgatga acaagcggga ggaccggtta aatcaggacg acgtggccat
      841 cctaatgatc atcggggatt tcacggcact ggccatcgag caggcgcgat tatttcagga
      901 agccaaatta gcggaagtgg cacgaatcct gggagacatt gggcatgacg tgaaaaatat
      961 gctgatgccg gtcctgtgtg ggacgtctct cctgagagat gagatgaatg acgtcttcgc
     1021 cgatcttcca gcgtttgatc ctgacaaagg ccgggcgagc catgaattat gcctggaagt
     1081 catcgggatg gtggccaata acgccaaacg gattcaagag cgggtaaagg aaattgccga
     1141 ttgtgtgaag ggtttgacga gccccccacg ctttgcccct tgtaaacttc accatgtaat
     1201 cgcttcggtg tttgatacct tgaagttggc ggctcaagaa aaagggatta ccctggctca
     1261 tgaaggaatt ctggaattac cggtgataca agccgatgaa tcccggctgt tcaatgcctt
     1321 ttataacctt gtcaataacg ccatttccga ggttcccagt ggcgggtcta tcacgataaa
     1381 gggacaaatg gaagctatgg gaaagacggt tcacgtgtcc gtgattgaca ccgggcgtgg
     1441 catgcccgcc gaaattcgag atacgttatt caccgccagg gccataagcc ggaaacaggg
     1501 tggaaccggg ttgggaacaa aaatagtcaa ggatgtcatc gaagcgcatt cgggagtcat
     1561 tgcggtagag agtgaagtga atagaggcac cgtttttcac attcatttgc ccattgaaat
     1621 gtcgacgacg ttttcttcgg atcaggtgtc ttcttaaatc ttttgaaata cggatacgag
     1681 gcgtgaacaa tgttcttccc cgttcattgt cccatccaaa acatctactc gcttgtggtg
     1741 accaacaaaa catagcagat atcttcccgt tttctggttc attcatatcg tgcttgagct
     1801 gttaagggga tctctgtggg ccattcacct cttcgttctt ccgcctttca aatcggggtc
     1861 ataaccctca tggtttttct gggtgttatg gggcttcggt gggaaggatt tctggaaagc
     1921 gcggaactgg atgcctacga ttggtcaatg cgtcttcgtc cgaccaacac acggccgccc
     1981 cccccgatta cgctggtgtc gattacggac caggatattc gaacattagg gcattggccg
     2041 gtaacggatg aagtccttgc ccgagccctg gacgtcatga cggcatttca ccctcgagcg
     2101 atcggagtgg atatctatcg ggatttggaa gtgccgcccg gtcggcagga attggaccgg
     2161 gtacttgagg cgcatccgga aattctgatg gtcatgaaat tcggaaaaat tgagaagggg
     2221 ggtattcccg gtccggccat gttgcaaggc acggatcgtc tcgggtttaa cgatgtggtc
     2281 gtcgattccg gcgggatcgt tcgccgtggt ctcttattcc tggatgatgg gaccaatttc
     2341 tatcggtcat tttctttact gctggccctc caatatttac agcaggagaa aatcgtcccc
     2401 aagccggccg ttgagaattc tgattggttg caacttgggg aaacggtcat tcgccctttc
     2461 gaagcgcacg atgggggcta tgtggaagcc gatgcccagg ggtatcaata tctgttgaac
     2521 cttgaacggg ggaaaaacgt ctttccgacc atttcccttg gagacatact ggaaggaaat
     2581 tttaatccgg agctgcttca agaccacatc gtgcttgtcg gggtgatcgc ccagggcgtc
     2641 aaggattatt tttatacctc acaatgcggg acactcacgg tgtgtccccc aattcccgga
     2701 cttgaactgc atggatatat ggtgcaccaa ctgctccgct tagcgaaggg agagggccag
     2761 gcccccattg cgacatttcc agattccctg gaaacagcct ggatcgggct atgggtgctt
     2821 gggggcggat tcatcggcgt gcgggttcgg ggtgcctggc gtttttctct ggctgtgctc
     2881 atggggctgc tgctgctaag cggagtggtg gtgagcggaa tgatgtatgg gacgtggatc
     2941 ccgtttttcc cggcggtcat tggattggtc gtcaatgcca tggtggtgac agcctggctt
     3001 tcgaaccggg aaaagcagga ccgggcgctc ctgatgtcgc ttttttcccg gcatgtctct
     3061 tcagaagtgg cccaggctgt ctgggagaag cgggaccaat ttttggaaaa gggccggtta
     3121 cgcccacaaa aattggtggt gacgacgttg tttacggatc ttgaagggtt cacgactgtg
     3181 gcagaaaaca tggaacccga cacggtactg gactggctca acacatatat ggaacgaatg
     3241 gtaaaaatca tcaccaacca tggtgggatg gttgatgatt atcatggaga tatgattaaa
     3301 gcggatttcg gggtcttccg gctggaacaa acggaagcca acaagcggca ggatgtcaac
     3361 aatgcagtca gttgtgccgt ggctctggaa caggaaatgc gacatcttaa tactcaatgg
     3421 cagcagcagg ggttgcccga ggtgcgaatg cggataggca tgcaatcggg gccggtggtc
     3481 gtcggaagtc tgggcagtga acaacggctg aaatttacga ccattggaga ctccgtgaat
     3541 gttgcctccc ggttggaaag tttccaaaaa gattcgttgg agacctggtc caaagatgag
     3601 gtctgccgga tccttattgg agagacgacc aaacactacc tcggggacca tccatggaga
     3661 cttaaagaag tcggcgtgct cacgctaaga ggaaaagcca accgcatttc cgtttaccgg
     3721 ctgttcacga acacgggcag tccggaacca cgttcggcga tttccatcga acaagccgga
     3781 ggataggtca tgggcctggg atttt
//
LOCUS       DATIAL010000534         3950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_419520, whole genome shotgun sequence.
ACCESSION   DATIAL010000534 DATIAL010000000
VERSION     DATIAL010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419520"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VLA60_16415"
     CDS             complement(<1..292)
                     /locus_tag="VLA60_16415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HSF10996.1"
                     /translation="MKKRAGATSLNARSRSSASPQPKPPQKPGDQKLWGGRFQGQTHQ
                     LVETFTASINVDRRMYEYDIEGSIAHCKTLQRAKVLPQRECQTIIRGLLTIRE"
     gene            complement(298..1293)
                     /gene="argF"
                     /locus_tag="VLA60_16420"
     CDS             complement(298..1293)
                     /gene="argF"
                     /locus_tag="VLA60_16420"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HSF10997.1"
                     /translation="MSSPRQSSRTRKSPPSAQRKKRTDLPKDLLTVADIPRDTIHHLI
                     ALAQKMKAARRQGRTTFPLKGKTLGLIFEKPSTRTRVSFEAGMNQLGGQAIFLASEKI
                     QLSRGESLADTAKVLTRYLDGLVVRTFNQASLEEWARHTSIPVINGLTDDCHPCQALA
                     DLLTIVEHRGHAKGLKLAYIGDGNNITHSLIEIGGKVGMHVIVGCPKGYEPDPRIVAA
                     AQEESQQTGARIEITHDPNIAATDADVIYTDVWISMGQEHQQEPRMEALAPYQVNGRL
                     MQQAKPNALLMHCLPAHRGEEITEDVLDGPQSVVLDQAENRLHIQKAILLQWLGR"
     gene            complement(1297..2490)
                     /locus_tag="VLA60_16425"
     CDS             complement(1297..2490)
                     /locus_tag="VLA60_16425"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HSF10998.1"
                     /translation="MITSELQQNTGRFLMNTYTRLPISLVRGNGCLVYDAEGREYLDC
                     LAGIAVNILGHAQPDLVETITRQARQLIHTSNLFYTEPQTRLAQKLVELSFADKVFFC
                     NSGTEANEAAIKLARRYAHNTFGPERFEILTMVNSFHGRTLASLTATGQPKLQEGFGP
                     LVPGFRYVPFNDLEALKANLTPQTAAVLLEPIQAEGGIMVPHPSYLKGLRELCTENQI
                     LLIFDEVQTGMGRTGTLFAYEQFGIQPDIMTLAKGLGGGLPIGACLATDNVAAAFEPG
                     THASTFGGNPLACAVALKVLELLIEGGKLEQGLVAGRYLAKGLGSLKEQFSCIHEARG
                     LGLLQGLELTIDGKPLVLECLDRRVLINCTMGKVLRFVPPLIISNAQIDRLLSVLSDV
                     LSKHR"
     gene            complement(2652..3512)
                     /locus_tag="VLA60_16430"
     CDS             complement(2652..3512)
                     /locus_tag="VLA60_16430"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HSF10999.1"
                     /translation="MSRTLDPSLAGTPQRDYPTMILFGSIVLTTIVGLPLYAYFYDFS
                     WVDWTMFIMLYLFTGLGITVGYHRLITHRSFKCPNWIKATFLIAGGMALENSALKWAS
                     DHIRHHARCDQKEDPYNATLGFWHSHCGWIFWKDPNRDPKYATRLLQDPLILWQDKYY
                     LPILLSGLVLPFVVGFLYNGWIGAIGCFLLAGLARTFFVLNSTFFINSICHIWGDQPH
                     GTADSSRDSWWISLLTFGEGYHNYHHMYQSDYRNGVRWYNFDPSKWLIWTLSRLGLAY
                     DLRRQSPNQP"
BASE COUNT         1000 a          898 c         1009 g         1043 t
ORIGIN      
        1 cctcccgaat ggtcaggagt cctcgaataa tggtttggca ttctcgctgc ggcaggacct
       61 tagcccgttg taaagtttta caatgggcga tgctgccttc aatgtcgtat tcatacatgc
      121 ggcgatcaac gttgatcgag gcggtgaacg tttccactag ttgatgtgtt tgaccttgaa
      181 atcgcccccc ccagagtttt tgatcgccgg gtttttgtgg cggctttggt tgaggtgatg
      241 ctgaagaacg agatcgggcg ttgagggaag ttgcgccggc acgttttttc atgaagacta
      301 tcttcctaac cattgaagca aaatagcttt ttggatgtgt aggcggtttt ctgcttgatc
      361 cagaacgact gattgtgggc cgtccagcac gtcttcggtt atttcctctc cccgatgggc
      421 cggcaggcaa tgcatgagga gggcattggg ttttgcctgc tgcatgagtc tcccgttgac
      481 ttgatagggc gccagagctt ccatgcgtgg ttcctgctgg tgttcctggc ccatgctgat
      541 ccacacatct gtataaatca catcagcgtc cgttgcggca atgttaggat catgggtgat
      601 ttcaattctg gcacctgttt gttggctttc ctcctgggct gctgcgacga tacggggatc
      661 aggttcatag ccttttgggc atcctacgat cacgtgcatg ccgaccttgc ccccgatctc
      721 gatgagggag tgggtgatat tgtttccgtc tccaatatat gccagcttta agcctttcgc
      781 gtggcccctg tgttcgacaa tggtcaaaag atcagctaat gcctggcagg ggtgacaatc
      841 gtccgttaac ccattgatga cgggaatcga ggtatgtcgg gcccattctt ctaaagaggc
      901 ttggttaaat gttcttacga caagtccatc caagtatcgc gttaggactt ttgccgtatc
      961 ggctaaactt tctccacggc tcaattggat tttttcggat gccagaaata tagcttgtcc
     1021 accaagttga ttcattccgg cttcaaacga aaccctggtc cgcgtagagg gtttttcaaa
     1081 aataagaccc aacgttttgc ccttcaacgg aaaggtggtg cgcccctgtc ttctggcagc
     1141 cttcatcttt tgagcaagag caatcaaatg gtgaatcgta tccctgggaa tatcggccac
     1201 cgtcagcaag tccttgggga ggtccgtcct cttcttgcgc tgtgcggaag ggggggattt
     1261 tcgggtacga gaagattgcc ttggtgacga catgatttat cgatgcttgg aaagaacgtc
     1321 agaaaggacg gacaggagcc gatcaatctg tgcgttactg atgattaagg gaggcacaaa
     1381 tcggaggacc tttcccatag tgcagttgat taggacacgt cggtcaaggc attccaggac
     1441 gagaggtttt ccgtcaatgg ttaattctaa accctggagg aggcccaggc cacgggcttc
     1501 atggatgcag gagaattgct ctttcaagga gccaagtcct ttggctaaat agcgaccggc
     1561 aaccagccct tgttcaagtt ttccgccttc aatgaggagt tctaagactt ttaaggctac
     1621 cgcgcaggct aaagggttgc ctccgaacgt ggaggcgtga gtgcctggtt caaatgccgc
     1681 tgcgacgttg tcggtagcta gacacgcccc aatggggaga cctcctccca agcctttagc
     1741 taacgtcata atgtcgggtt gaattccgaa ctgctcataa gcaaacaacg ttcccgtgcg
     1801 acccatcccg gtttggactt catcgaaaat tagaaggatt tggttctctg tacagagctc
     1861 tcgaagtccc ttcagatagg aaggatgggg aaccatgatc ccaccttcag cctgaatggg
     1921 ttctaacagc acagctgccg tttggggagt taaattggct ttcaaggctt ccagatcatt
     1981 gaatggaacg tatcgaaagc ccggtacaag cggtccaaat ccttcttgta attttggctg
     2041 cccggttgca gtaagactcg ccaatgttcg tccatgaaag gaattcacca tggtcaaaat
     2101 ttcaaaccgt tccggaccaa aggtgttgtg tgcatatctc ctggctaact ttatagcggc
     2161 ctcgttggct tctgtcccgc tattacaaaa gaacactttg tctgcaaagg acagttccac
     2221 cagcttttgg gccagtctgg tttggggttc ggtataaaac aggttggagg tatgaatgag
     2281 ttgccgggct tgtcgcgtga tggtctccac aagatccggt tgggcatggc ctaggatatt
     2341 gacggcaatc cccgcgagac aatcgagata ttcccggcct tctgcatcat acaccagaca
     2401 tccgtttcct cgaacaaggg aaatgggtag gcgggtatag gtattcatca gaaatctacc
     2461 cgtattttgt tgaagttcac ttgtaatcat attgtcaaaa acccgcgtga atgcaggcga
     2521 atttatcaca cggcttctta ggttcgcaat gttgagctgg gtgggagatt ggccggataa
     2581 caaaaaagca ggagagaggc tccgtccgag attccaattc aggtggagga gcctttaaat
     2641 gctaaatggc gttacggctg gttaggagac tggcgccgaa gatcataggc taagcccaat
     2701 ctactcaacg tccatataag ccacttggat ggatcgaaat tgtaccagcg aactccattt
     2761 cgatagtcac tttgatacat atgatgatag ttgtgatatc cctctccaaa tgtgagtaat
     2821 gatatccacc aactatcccg gctcgaatca gctgtcccat ggggttgatc gccccagata
     2881 tggcaaatgg aattgatgaa gaatgttgaa ttgagaacaa agaacgtacg tgcaagtcca
     2941 gccaggagga agcacccgat agctccaatc cacccattgt ataaaaaccc tacgacgaat
     3001 ggcagcacaa gtccagacaa aagaatggga aggtaatatt tgtcctgcca caatatcaga
     3061 ggatcctgca gaaggcgggt ggcatatttg gggtcacgat taggatcctt ccagaaaatc
     3121 cagccacaat ggctgtgcca aaagcccagc gtggcgttat agggatcttc tttctggtcg
     3181 caccgggcat ggtgcctaat atggtcgctg gcccatttta acgctgagtt ttccaacgcc
     3241 ataccgcctg cgattaagaa ggtggctttg atccagttcg ggcacttaaa gctccgatgg
     3301 gtaattaacc ggtgatagcc caccgtaata cctaatcctg taaataggta aagcatgata
     3361 aacatggtcc agtctaccca ggaaaaatcg taaaaataag catataatgg gagaccgacg
     3421 attgtggtca acacaattga cccaaacaga atcattgtgg ggtaatctcg ttgtggggtt
     3481 ccggctaagg aagggtctaa cgttcgtgac atagctgtct aagtcctctt atatctctaa
     3541 tcaccggagt gcctgttccc catatatatg ggaaattgaa cggtgagaga tctgttagtg
     3601 aaataaaacc taggattgaa atctctttta catatacgaa agtgggttgt tgaaaccgac
     3661 taacacggga tttgattttc ccgtgtaaaa gagtggtttg agagtataat gtaacaattt
     3721 agttggaaag aggcaagacc atagcagaaa tcaatggtga taccattcat gtatatagaa
     3781 taccagatgg attgaaaaaa actaggggac cctctcaaac caaggccccc taaatattac
     3841 tgtctgtggt tattgttgaa ttcccactaa atatttctga accattatca ttggttatgt
     3901 gctgtgttca atgagtcact ggcaagaata tcggaactca ccaagctata
//
LOCUS       DATIAL010000535         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_419641, whole genome shotgun sequence.
ACCESSION   DATIAL010000535 DATIAL010000000
VERSION     DATIAL010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419641"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VLA60_16435"
     CDS             <1..572
                     /locus_tag="VLA60_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate oxidoreductase subunit beta"
                     /protein_id="HSF11000.1"
                     /translation="RVSEKCIACYPRVEGKDPLTGGEPMETRCMAACVGKIRLQGLVK
                     VGDDGLWAEDRWNPLYYAIRVEQVALPLYPQWGTEPNGFYIPPRQAPRGYIRQMFGPG
                     VDNAIEKYLVPSRELLAVLQLWRASQQIIFRYDVIPGPKVFETQIHGRKFEMYNDTVL
                     GFNKSGKEAVRQQVEEPIYIRPAERVNWL"
     gene            1220..1549
                     /locus_tag="VLA60_16440"
     CDS             1220..1549
                     /locus_tag="VLA60_16440"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HSF11001.1"
                     /translation="MFGETVVIAYCPRFFIGRVCGIWGPTVLFFPQGIEHAVVTMFVI
                     PVGMVMGAEVDMSHWRFLNKIPVMLGNLVGGMFFTGLAIYYAHGSATIAPQQVQSVNH
                     ASSSISA"
     gene            1589..>2546
                     /locus_tag="VLA60_16445"
     CDS             1589..>2546
                     /locus_tag="VLA60_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF11002.1"
                     /translation="MPAMFPKRLRGWHFILFNIALGLGHILVMFNAGSYVALLPHVAG
                     DLEGVLPSFMTWAQTDFMIALALGLPVGRALANRFGNYRVFIGAFLVYAVASYLGAIS
                     QTHWQFLNARIIQGFSGGLTLFLSQDMMLREYPDRLKSLGLALWGLITITPFTIGLPA
                     GGWLADDLGWWRYLFYTDVVVSLVVVGLLGALLYGRGFRRQPIRFDVVGFFLLAIVLG
                     SMQTILNMGNDFDWLDSSFLRGILIVLIVALPCFIIWELGERHPVLDLRLFADRNFTI
                     GVICLVVGFFSIQGLLTLLIIQLQLLMGYSSLLAGMALLPMVL"
BASE COUNT          506 a          592 c          765 g          683 t
ORIGIN      
        1 cgcgggtgag tgagaagtgt attgcgtgct atccgcgggt ggaaggcaaa gacccgctga
       61 ccggtgggga gcccatggaa acacggtgta tggcggcgtg cgtcgggaag atccggctgc
      121 aagggctggt gaaggtgggc gatgacgggc tctgggcgga agaccgctgg aatccgttgt
      181 actatgccat ccgggtggaa caagtggcgt tgccgttgta tccgcaatgg ggcacggagc
      241 ccaacgggtt ttacattccg ccgcggcagg cgccgcgggg ctacatccgg cagatgttcg
      301 ggccgggggt ggataatgcg attgagaagt atctggtgcc gagccgggaa ttgttggcgg
      361 tcttgcagct ctggagagct agccaacaga tcatcttccg gtatgacgtg attccggggc
      421 cgaaagtgtt tgaaacccaa atccatgggc gaaagtttga gatgtacaac gacacggtgt
      481 tggggttcaa caaatcgggc aaggaagcgg tgcggcagca agtggaagag ccgatttaca
      541 tccgcccggc ggagcgggtg aactggctgt aagttccatg gtattcatgc agtgggagac
      601 aaccggaacc atcaattttc ggtgaggcag gaggccggct ggcgactgaa aaatgcgtgt
      661 cagggaaaaa tcgccacccg gtctctctcc tgttaagggg agaagccgga aaggggggat
      721 gttatgaatt gtcggcgttg tgctggtctc atgcattggc taaatatggg agatcatctt
      781 aatgattcag gacagttatg gaccatggct tggcaatgct ttaactgtgg agaaattgtc
      841 gatcctttga ttttacaaaa tcgggggaac gcacccaggc ccaaggtggg aaaggcccgc
      901 ctaaaaacgt tttcaccatc caggcagata ctgcgggaaa atggtcaaac catagaagcc
      961 taacgaagaa ccgcaagtcc gctgctggaa gagcacaaag gggtgaatac gtttcatctc
     1021 cttgtgctct tcctcgttga atatagggaa aataataaag aacctttttc atcacaaagg
     1081 attcgttcat cgggggtcca aattattatt cgaagaaaga gcgttttttt gatgggtacg
     1141 acgggacctg gaattcagat aaggaggttg accatctttc cataagggag aagcatcatg
     1201 gaggatgtca aatctttgga tgtttgggga aaccgtggta attgcctatt gccctaggtt
     1261 ctttattggg agggtatgtg gcatttgggg gcctacggtt ctgttcttcc cgcaggggat
     1321 tgaacacgcg gtggtgacca tgtttgtcat cccagtcggt atggtaatgg gagctgaggt
     1381 tgatatgtct cactggaggt ttttgaacaa gattcctgtc atgctgggca acctggttgg
     1441 cggaatgttc ttcaccggcc tggcaattta ttatgcacat ggctcagcca ccatagcccc
     1501 tcaacaggtt cagagcgtca accatgcatc ttcttctata tccgcgtaag cccaatggaa
     1561 gtccctgcct gtgaggcaga ggtaagccat gccggctatg tttcccaaac gcctgcgagg
     1621 ctggcacttt attcttttca acattgcgct tggccttggg cacattctcg tgatgttcaa
     1681 tgccgggtcc tatgtcgcgc tattgcctca tgtggccggc gatctggaag gcgtgttgcc
     1741 gagcttcatg acctgggctc agacggattt catgatcgcg ctggcgttgg gccttcccgt
     1801 tggccgcgcc ctggctaacc ggttcggtaa ttatcgtgtt tttattggcg cctttctagt
     1861 gtatgccgtg gcttcgtatc ttggcgccat cagccagacc cattggcagt ttctcaatgc
     1921 ccgtataatc caggggttct caggcggcct cactcttttt ctctcgcaag acatgatgct
     1981 ccgcgagtat ccggaccggc tcaagtcctt ggggttagcg ttatgggggc tgattactat
     2041 tactcccttt acgattggtc ttcctgcggg tggttggctt gcagatgatc tcgggtggtg
     2101 gcgttatctt ttttatacgg atgtcgtggt ctccttggtc gtcgtcggtc tcctgggggc
     2161 tttgctgtat ggtcggggtt tccgccgcca gcctatccgg tttgacgtcg tgggattttt
     2221 tctgctggcc atcgtgttgg gcagcatgca gactattctg aacatgggga acgattttga
     2281 ctggcttgac tcgtctttcc tccgtggtat cctgatcgtc ctcatcgtgg ccttgccttg
     2341 ttttatcatc tgggaactcg gcgaacgtca cccggtgctg gatctccggt tatttgccga
     2401 ccggaatttt accatcgggg tgatctgttt ggtggttggg ttcttctcta tccagggact
     2461 cctcacgctt ctgattatcc agcttcaatt gttgatgggc tattcgtccc tccttgccgg
     2521 tatggcgctc ctcccgatgg tgttgt
//
LOCUS       DATIAL010000536         4709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_740539, whole genome shotgun sequence.
ACCESSION   DATIAL010000536 DATIAL010000000
VERSION     DATIAL010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740539"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /locus_tag="VLA60_16450"
     CDS             complement(<1..1392)
                     /locus_tag="VLA60_16450"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11003.1"
                     /translation="MTTSAQHPYHPIIYVRGFAATQSEIEETVADPYMGFNIGSTKSR
                     TAWTGDVKRFYFESPLVRLMSDHEYEDVFVDGDDLVLAERTDYPVPYRSIIIYRYYDE
                     ASKDFGDGKTPPIEHFAKGLGTLILRLREKVCANKDNKVTSKDFRVYLVAHSMGGLIC
                     RAFLQNTKLGSAEARGAVDKVFTYATPHNGIDMRIVRNVPGWLSFGDINNFNREKMAS
                     YLAVPKGDDVSVVKNFPPERIFNLVGTDSHDYTVAGGISSWAAGDASDGLVRLENATT
                     HGAGPDGKDVSSPRAFVHRSHSGHYGIVNSEEGYQNLTRFLFGALRVDGILEIDDITL
                     PVEVQKALDDGKTVRASYQFEIAASVRGCQWQMTRREVRENSAIFRTYDDLFPGKDRS
                     KRKPDRTKSPHLFSIFLDPSKSVKASKSVSFAFDIKVLVPDYVVDGLLFMKRHYEGGF
                     IYRELILVEAFPDE"
     gene            1668..2663
                     /locus_tag="VLA60_16455"
     CDS             1668..2663
                     /locus_tag="VLA60_16455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF11004.1"
                     /translation="MLHRLSRWNPIRLSSFFSPRNEHDRNAERPPQRLATRYLRTARS
                     ASVLLISLTLTISLTDRLWANEEPLTKPSWSQEDQKILQKIHTILVAGTVQTWLNVPS
                     PPYNVGVTLKLKLEDAGFQVVFDPKQTHDAILYIQYEEIPSGQFQVLEQATAIRYDMK
                     LVHDQLGQVFSHHFEAEPNAIPLGSLYWDAIGNLEEDPYYCFVGDLIRGYIDGDQNAQ
                     DMLMEVLVRPFTQKELVNAAGARGATQAIVQQRARLKIIQELGQGAFHTPEAQAVLWT
                     VAKKAQPNERGAAMTQLGEIGDATFLSPLTTLLENETDPEVRSAGEHAIQLIESR"
     gene            complement(2810..4120)
                     /gene="nhaA"
                     /locus_tag="VLA60_16460"
     CDS             complement(2810..4120)
                     /gene="nhaA"
                     /locus_tag="VLA60_16460"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HSF11005.1"
                     /translation="MKKTPVDTWLTDPLHSFLGRQSTSGIVLFVAALMALIVANSPLA
                     DAYHHFWHNEISVGFNDFVISKTLHHWINDGLMAVFFFVIGLELKREIMAGELSNPRD
                     ALLPIAAGVGGMVVPALIYLAFNPSGEATDGWGIPMATDIAFALGIISLLGDRVPLSL
                     KVFLTALAIADDLGAVLVIAFFYTSHIDFLNLAAGAGFMILLVTSNLLGVRNILWYSL
                     LGIGGLWLAFLLSGVHATIAAVLAAFTIPANVKISDKGFVMRMKGLVNKFEAANINND
                     VTLVTDEQLHLLEDIRSVAKGALTPLQRLEHGMHSFVAFVVMPVFALANAGISFPADF
                     LNQLASPVTLGVASGLLGGKVLGIMGMCYILIHFQWASFPEQTGWRHLIGAAMLASVG
                     FTMSLFITGLAFNDDVLILQAKLGILLASLIGGISGYYLLRRAG"
     gene            4119..4277
                     /locus_tag="VLA60_16465"
     CDS             4119..4277
                     /locus_tag="VLA60_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11006.1"
                     /translation="MLRNYHEERECETGCQIDFSWHGIRVPQLPDSLERYAQYHLGQC
                     HPLAYHFA"
BASE COUNT         1190 a         1293 c         1195 g         1031 t
ORIGIN      
        1 ttcatcagga aaggcctcga ccagaatcag ctcacgatag atgaacccac cctcatagtg
       61 gcgtttcatg aacagcaggc catccacgac gtaatcgggt accaagacct tgatgtcgaa
      121 ggcaaagctg accgatttgg atgctttgac gctcttactt ggatccagaa aaatggaaaa
      181 gaggtgtgga ctcttcgtgc ggtcgggttt gcgcttgctg cgatccttcc ccggaaacag
      241 gtcatcgtag gtgcggaaga tggcggaatt ctcgcgcacc tcgcgccgcg tcatctgcca
      301 ctgacagccg cgcacactgg cggcaatttc aaactgatac gacgcccgca ccgtcttccc
      361 gtcgtcaagg gccttttgca cctccaccgg caatgtgatg tcatcgatct ccagaatgcc
      421 atccacccgc aaggcgccaa acaggaaccg tgtgaggttc tggtagcctt cctcggagtt
      481 cacaataccg taatggcccg aatgactcct gtgcacgaac gcgcgagggg aactcacatc
      541 cttgccgtcg ggacccgcgc catgcgtagt ggcattctcg agacgcacca gcccgtcgct
      601 ggcatcaccc gccgcccacg acgaaatgcc gccggccacg gtgtaatcgt gtgaatcggt
      661 gccgaccagg ttgaagatgc gctcgggcgg gaaatttttg acaaccgaaa catcatcacc
      721 cttgggcacc gcaagatagg aggccatttt ctcgcgatta aaattattga tatcgccgaa
      781 tgaaagccag cccggcacat tacgcacaat ccgcatatca atgccattat gcggtgtagc
      841 ataggtaaag actttgtcca ctgcaccgcg cgcctccgct gagccgagtt ttgtattctg
      901 tagaaaggcc cggcagatca gccctcccat ggagtgagcc acgagataca cgcgaaagtc
      961 tttggaagtg accttattgt ccttattcgc gcagaccttt tcgcgcaagc gcagaatgag
     1021 cgtgccgagg cctttggcga agtgctcaat aggcggcgtc ttgccatctc caaagtcctt
     1081 cgaggcttca tcgtaatagc ggtagatgat aatcgagcgg tatgggacgg gatagtcggt
     1141 acgctcggct aacacgaggt cgtctccatc cacaaagaca tcctcatatt cgtgatcgct
     1201 cattaaccgc acgagcggcg actcaaaata aaagcgcttc acgtcgccgg tccatgccgt
     1261 gcgcgacttg gtcgaaccga tgttaaaccc catataaggg tcggccaccg tttcctcaat
     1321 ctcgctctgt gtcgctgcga agccccggac atagataatc gggtggtagg gatgctgcgc
     1381 ggatgtcgtc atgaaggtac ctccgaatta cgattgcacg aatgtcagtg tgaacgagcc
     1441 ttcttctatt ccaatttatt tcccattgca gatgcgggga ttggctgaga agagattttt
     1501 cgtcaattca ggaaattagt caaggtatga agatgcaaaa gaaggacaga agggtcagcc
     1561 tgacatcagg aagattttga attccgcatc aactcttgat aggctatatc taatacgtcc
     1621 ttgatggccg gacatttcaa cattcgtcca acccaatgga gcgtctcatg cttcaccgtc
     1681 tcagccgatg gaatccgatc aggctttctt cctttttctc tccacgaaac gaacatgacc
     1741 ggaatgccga gcggccaccg cagcgattag cgacccgata cctgaggact gcgcggagcg
     1801 cgtctgtcct actgatcagt ctgaccctga cgatttctct cacggataga ctgtgggcaa
     1861 atgaagagcc actgaccaag ccctcctggt ctcaggagga tcaaaagatc ctgcaaaaaa
     1921 ttcacaccat actcgtcgcc ggcaccgtcc aaacctggct gaatgtgccg agtccaccct
     1981 acaatgtggg ggtgaccttg aaactcaaac tggaagatgc cgggtttcaa gtggtctttg
     2041 atcccaagca gacacatgat gccattctct acattcaata tgaggaaatt cccagtggac
     2101 agtttcaggt attggaacaa gcaacggcta tccgctacga catgaagttg gtgcatgacc
     2161 aattgggtca ggtcttttca catcactttg aagcagaacc caatgcgatt cccctcggga
     2221 gtctgtattg ggacgcgatc ggtaatctgg aggaagaccc ctattactgt tttgtaggag
     2281 atctcattcg gggatacatt gatggagacc agaatgccca ggacatgtta atggaggtgt
     2341 tggtaaggcc ctttacccag aaagagttgg ttaatgcggc cggagctcga ggcgccactc
     2401 aggctattgt ccaacagcgc gccaggttga aaattattca ggaactagga cagggggcgt
     2461 ttcatacccc tgaagcgcag gctgtcttat ggacagtcgc caaaaaagcg caacccaacg
     2521 aacggggggc ggccatgacc caacttggcg agattggcga cgccacgttt ctctctccac
     2581 tcacaacatt attggaaaac gaaaccgatc cggaagtccg atcggctggg gagcacgcga
     2641 tccaacttat tgaatcccgc taacgtacac tagaaaaaat tccgagtgtg tatagcagtc
     2701 ctgtagagat agccctcgca aataatctta ctgaagcctt tccaggctct gtttttatcc
     2761 tttttgcaac ttcccgcact gcatattcca cagttttcta ttgagaaatt caccctgccc
     2821 tccgcagcag gtagtaccca gaaatcccac cgattaacga ggcgagcagg atgcccaact
     2881 tggcttgaag gattaagaca tcatcattga aagccagtcc ggtgatgaac agcgacatcg
     2941 tgaaccccac cgaggcaagc atcgcagcac caatgaggtg ccgccagcct gtctgttccg
     3001 gaaaggaggc ccactgaaaa tgtatcagga tgtagcacat gcccatgatg cccagcacct
     3061 ttcctcccag cagccctgat gccactccta atgtgaccgg actggccaac tgattcagga
     3121 agtcagctgg aaagctgatt ccggcattcg ccaacgcaaa gaccggcatc accacaaacg
     3181 ccacgaagga gtgcatgcca tgttccaacc gttggagcgg agtcaaggca cccttggcca
     3241 cagagcggat gtcttccaac aggtggagtt gctcatcggt caccagcgtc acgtcattat
     3301 tgatgttcgc agcctcgaat ttgttcacta accccttcat gcgcatgaca aaccccttat
     3361 ccgagatctt cacattggca ggaatcgtga acgcagccag caccgcggca atggtggcat
     3421 gaactcctga taagaggaac gccagccata ggccaccaat tcctagcagg ctataccaca
     3481 gaatgttgcg tacgcccagc aggttggacg tcaccaggag gatcatgaat cctgcaccgg
     3541 ctgccagatt cagaaagtcg atgtgggacg tatagaagaa tgcgataacc agcaccgcgc
     3601 cgaggtcgtc cgcaatagcg agcgcggtga gaaacacctt gagcgacaat ggtaccctgt
     3661 cgcccagcag ggagatgatg ccgagcgcga aggcaatatc tgtggccatg gggatacccc
     3721 atccatcagt cgcttcgccc gagggattga aggccaggta gatgagcgca ggcacgacca
     3781 tgccgcctac tcccgctgcg atagggagca gcgcatcacg gggattgctc agttcacccg
     3841 ccatgatctc acgcttcagt tccaacccga tcacgaagaa gaagacggcc attaacccat
     3901 cattgatcca atggtgcagg gtcttactga tcacaaaatc gttaaacccg actgagattt
     3961 cgttgtgcca aaaatgatgg taggcatccg caaggggaga attggccacg atcaatgcca
     4021 tcagggcagc aacgaatagc acaatgccac tggtggactg cctaccaaga aagctgtgaa
     4081 ggggatctgt cagccaagta tcgactgggg ttttcttcat gcttcgaaac taccatgaag
     4141 agcgggaatg cgaaaccgga tgccagatag atttctcatg gcatggaatc cgcgtcccgc
     4201 aacttcctga ttcccttgag cggtatgccc aataccatct gggacaatgc catcccctcg
     4261 cctatcattt cgcctgagat tgggacaggc gccaccacac gtaccaccgc acggtgggcc
     4321 ttcaaatcaa gatgagggtc atggaggatg aagagggaga taatggaatt ctcttgtata
     4381 gaaaattcca tcggaccaag caaaccgtga acgaagaccg tatgcttctg gtagccctca
     4441 tccaccttta accacctggc atcttgcgcc ggatgccaag gatctgagtc caggtcgaga
     4501 aacctgagac tcaacaagat tctttgtttc tctcaggttg tctctaataa cctccacgtt
     4561 cttctctgct attgccccat gggagaggaa agatttttcg gcaggaaatc ttttttgaca
     4621 taatcgttgg gagggtcaaa ttccttcgct gcgaccgact tctcctccac tccggtcaac
     4681 cggctttctg tgacaatggt tccattgga
//
LOCUS       DATIAL010000537         5234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1494567, whole genome shotgun sequence.
ACCESSION   DATIAL010000537 DATIAL010000000
VERSION     DATIAL010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494567"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VLA60_16470"
     CDS             complement(<1..1433)
                     /locus_tag="VLA60_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HSF11007.1"
                     /translation="MNFIHQIFPQAIRVPPLVLGVLVCLAMANPVQGKALMPIHHELH
                     IELNPDTHSIAGSDTVHLSPSHRANSSVSFILHPQLTIDRVEFNGKLLPVPPPSEGQT
                     LQPGSTRRVIEIPLPALDGPDQPAILTLTYHGQIDDSPKASGGLRFVKPDDTNGHIGP
                     NGVYLTYETFWHPTWEQTLSTYDLTLSLPSDWEAITQGREVINTVTAGRRTTQWKVSL
                     PSEALTLAANHFVVQKHEWKGIQLATYLFKDDAHLAPQYIEATIDYLQMYTDLLGPYP
                     FTKFAVVENFFPSGLGLPSFTLLGEGVIRRGYTQPYSLGHEIVHSWFGNSVFNDFAQG
                     NWVEGLTTYLSNYYYDEAKGHQQEAFNTRRRMVYEYNLYAEPDKEYPVRAFHHKETRL
                     DNAIGYQKTAMVLHMLRQEMGEVAFFNGVRRIIQEGTGTYLEWNDLLRIFSQAAERDL
                     GWFFHQWIDQPGVPNLDIQDILVQEDPT"
     gene            complement(1512..2438)
                     /gene="ftsY"
                     /locus_tag="VLA60_16475"
     CDS             complement(1512..2438)
                     /gene="ftsY"
                     /locus_tag="VLA60_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HSF11008.1"
                     /translation="MKWVERLQKGLAKTRQTVQTSLRRLGRGHLDPVALEDVEISLLE
                     ADVGIHTVERFLEMLKDKTRLFSGTDPTAVLHTLLTDILRKGETEPLEQLIRRGPRPF
                     VILIIGINGVGKTTTIAKLGHRLKEQGLHTLFVAGDTFRAAAIEQLDIWGKRTGIEVI
                     KQKQGSDPAAVVFDGMVAAKARNVDVVLIDTAGRLHTKINLMDELKKIKRIIAREMPD
                     APHETLLVLDGTLGQNAIAQARQFHESLGVTGLALTKLDGTSKGGVVVAIVDELNLPI
                     RLIGVGEGEADLQDFQASAFVEALLPTENPDD"
     gene            complement(2488..2940)
                     /gene="ybeY"
                     /locus_tag="VLA60_16480"
     CDS             complement(2488..2940)
                     /gene="ybeY"
                     /locus_tag="VLA60_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HSF11009.1"
                     /translation="MAVWLRSHLRRVSVRPKTVCRLTQAVLQQAGYPSADLSLTFVGK
                     ARMQKLNRTYRQRDYATDVLAFPMQDALQSPLAFVGDVVICLPVALAQAPRFGNTADE
                     EILRLLIHGTLHLLGYDHETTEREAKRMTRKERTIFGRLSSPALRLLA"
     gene            complement(2947..3993)
                     /locus_tag="VLA60_16485"
     CDS             complement(2947..3993)
                     /locus_tag="VLA60_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HSF11010.1"
                     /translation="MRKIKLREGVDLANLFGPHDLHLKMIEGELQVRMAARGDEITLH
                     GQPESVLRAEHILRELADWTRTYYELKPDDISRAILATEEKRDTPLKPYTVSASALPT
                     KKGNVNPKTPNQQAYLEAINKSDLVIAIGPAGTGKTYLAMAMALAALTHKQVSRIILA
                     RPAVEAGEHLGFLPGDLFAKVHPYLRPLYDALYTMMDIDRANRLIERGEIEIAPLAFM
                     RGRTLDDAFVILDEAQNATTEQMKMFLTRLGLNSKAIVTGDITQIDLPDSQKSGLAQI
                     ADILLNIDGIQFVYFSEQDVVRHRLVKEIIKAYDRFEHQLSNNSPHNISPGHSTASPG
                     IHSKSDTNGERPTR"
     gene            complement(4087..4917)
                     /locus_tag="VLA60_16490"
     CDS             complement(4087..4917)
                     /locus_tag="VLA60_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HSF11011.1"
                     /translation="MPELPEVQVILNHLNRHVLGGTIAMLTIHRSDIVRTGHDLIPWF
                     QRSTITHIARKGKCLIFSCHRANETRYLLSEQGMTGLWFFNPALASTPQHIHCRIEIS
                     GVPATELHYWNPRRFGRLWLFTLPELEAFMHRRFGADAMDISAQSFIQLIQSCRGRLK
                     PFLLDQHRLAGIGNIYANEILFRARVHPQARGYRLGVSACQRLYDTMHTVLGEAIAAG
                     GSSIRDFRAPDGSYGRFQQAHQIYQKAGLPCLHGCPTLITRLQSERSSFFCSICQKRR
                     "
BASE COUNT         1217 a         1239 c         1474 g         1304 t
ORIGIN      
        1 gtgggatctt cctggaccaa gatatcctgg atgtccagat ttggtacgcc gggttgatcg
       61 atccattgat ggaaaaacca accgaggtct ctttcagccg cctgtgaaaa gatccggaga
      121 agatcgttcc actccagata ggtgcctgtc ccttcctgaa ttattctgcg aaccccatta
      181 aaaaaagcca cctctcccat ttcttgtctg agcatgtgaa gcaccatggc tgttttttga
      241 tagccaatgg cattatccaa ccgggtttct ttgtggtgaa aggcccgtac cggatattcc
      301 ttgtccggct ccgcatacag attatattcg tagaccatcc gccgtcgggt attaaaggcc
      361 tcctgctgat gccctttggc ctcgtcataa tagtaattgg agagataagt agtgagccct
      421 tccacccaat tgccttgagc aaagtcattg aagacggaat ttccaaacca ggaatgaacg
      481 atttcatgac cgagagaata gggttgggta tatccgcgcc gaataacccc ttcacctaag
      541 agggtgaatg aggggagccc aagtccgctt ggaaaaaagt tttccacgac ggcgaatttt
      601 gtaaacgggt aaggacccag aagatcggta tacatttgaa ggtagtcgat ggtcgcttct
      661 atatattgag gagccaagtg ggcgtcatcc ttgaaaagat aggtggcgag ttgaatgccc
      721 ttccattcat gcttctgcac cacaaagtga ttggcagcca acgtcagagc ttcactcggc
      781 agactcacct tccattgggt ggttcggcgt ccggcagtca cggtattgat aacttcccgg
      841 ccttgggtaa tcgcttccca gtctgaaggg aggctgaggg tgagatcata tgtcgagagg
      901 gtttgttccc atgtgggatg ccagaatgtt tcgtaggtca aatacacgcc gttgggtccg
      961 atatgtccgt tggtgtcatc cggtttgacg aagcgaagtc cacctgaggc ttttggagaa
     1021 tcatcaatct gcccgtgata ggtcagtgtg aggattgccg gttggtccgg accgtcaagg
     1081 gcaggcaagg gaatttcaat aacccggcgc gttgatcccg gctgcaaggt ctggccttca
     1141 gaggggggag ggaccgggag caatttgccg ttgaattcga cccgatcgat tgtgagttga
     1201 ggatgcagga tgaaggagac ggatgagttg gcgcgatgag atggagaaag atgaacggtg
     1261 tccgagccgg caatggaatg ggtgtcagga ttcagttcaa tatgtaattc atggtgaata
     1321 ggcatcaatg ccttgccttg gacgggattc gccatcgcca ggcacaccaa gacgccgagg
     1381 acaagtggtg ggactcgtat tgcttgcgga aagatttggt gaatgaaatt cacaagccga
     1441 tttccttggt caatttccga acaccatgtt tttctccgac agaatgctgt gtcaaagaaa
     1501 gctgggacgc gtcagtcgtc tgggttttct gtcgggagca aggcctcgac aaacgctgaa
     1561 gcctgaaaat cctggaggtc cgcttcccct tcccccacac cgatcaaacg aatgggaaga
     1621 ttgagttcat cgacgatcgc cacgaccact ccccctttgg aggttccatc cagtttggtc
     1681 aaggctaaac cggtgacgcc gagtgactca tgaaattgtc gggcttgtgc aatggcattc
     1741 tgccccagcg tgccatccag gacgaggagg gtttcgtgtg gggcgtcggg catttcccgt
     1801 gcaataattc gtttgatctt tttcaattca tccatcaaat taattttggt atgtagccga
     1861 ccggcggtat cgatgagaac gacgtccaca ttgcgagctt tcgctgcgac catgccgtca
     1921 aacaccacgg cggcaggatc ggagccctgc ttttgtttaa tgacctcaat acctgtgcgt
     1981 ttcccccaaa tatccagttg ttcaatcgcc gcagcccgga atgtgtcccc ggcgacaaag
     2041 agtgtgtgta acccttgttc cttcaaccga tgcccgagtt tggcaattgt cgtggttttg
     2101 ccgacaccgt tgatgccgat gatcaagatc acaaatggac gagggccgcg tcgaatgagt
     2161 tgttccagcg gttcggtttc gcccttccga agaatgtccg tgaggagagt atggaggacc
     2221 gctgttggat cggtcccact gaataaccgt gttttatctt tcagcatctc aagaaatcgc
     2281 tcgaccgtgt gtatgccgac atccgcttcc aggagactaa tttcgacatc ctccagggcg
     2341 acgggatcca ggtggccacg ccctaggcga cgcaatgacg tttgaacggt ctgacgtgtt
     2401 tttgctaacc ccttctgaag acgttcaacc catttcataa ctgatgttcc cgacgattac
     2461 ggcaaataag tgatgcatgg ctttttatta tgcaagaaga cggagtgccg gcgaagaaag
     2521 gcgaccgaaa atcgtgcgtt cttttcgcgt catccgtttg gcttcccgtt cggttgtttc
     2581 atggtcatat cccagcagat gaagggtgcc atgaatgagt aaacgcagga tttcttcatc
     2641 ggcggtgttg ccaaaccgtg gagcttgagc caatgccacg ggaagacaaa tcacgacatc
     2701 acccacgaat gccagtggcg actgtaaggc atcctgcatg gggaaggcta gcacatcggt
     2761 cgcatagtcc cgctgccggt acgtccggtt gagtttctgc atgcgagcct tgccgacgaa
     2821 ggtgagactc agatcagccg aaggataccc tgcctgttgt aacacggctt gtgtcagtcg
     2881 gcacacagtt ttggggcgaa cggatactcg ccggaggtga gaacgaagcc acacagccat
     2941 cgggtgttac cgcgtgggcc tctcgccgtt ggtgtctgat tttgaatgaa ttccgggaga
     3001 tgcggtcgaa tggccgggtg atatgttgtg cgggctgtta ttggacagtt ggtgttcaaa
     3061 ccgatcgtac gctttgataa tttctttgac caatcgatgt cggactacat cttgttcgga
     3121 aaaatacaca aattgaattc cgtcaatatt gaggagaatg tcagcaatct gcgccaatcc
     3181 ggatttctgt gaatcgggga gatcgatttg ggtgatatcc ccggtcacaa tggccttgga
     3241 attcagtccg agcctcgtca aaaacatttt catttgttcg gtggtggcgt tttgggcttc
     3301 gtccagaatg acaaaggcat cgtcgagcgt tcgtccccgc atgaacgcca acggggcgat
     3361 ttcaatttcg ccacgttcga tgagccgatt ggcccgatca atgtccatca tggtgtacaa
     3421 cgcatcatac aagggacgta aataggggtg cactttggca aagagatcgc ctggaagaaa
     3481 gccgagatgt tcccctgcct ccacggctgg ccgtgcgaga atgatgcgac tgacttgttt
     3541 atgcgtgaga gcggcaaggg ccatggccat cgccaaataa gttttcccgg tcccggcggg
     3601 gccaatggct atgaccagat ccgacttgtt aatggcctcc agataggcct gttgattggg
     3661 agttttcgga ttgacattcc ctttttttgt cggaagcgcg cttgccgaaa cggtataggg
     3721 tttgagtggg gtatctcgtt tttcttctgt cgccagaatg gctcgtgaaa tatcgtcggg
     3781 tttcaattcg taataggttc gggtccaatc agctaattcc cgcaaaatgt gttcggcccg
     3841 cagaactgac tccggttgac catgaagggt aatttcatcg cccctggctg ccattcgcac
     3901 ctggagctcc ccttcgatca ttttgaggtg cagatcgtgc gggccgaaaa gattggctaa
     3961 atcgacccct tctcgtagtt taatttttcg cacgtcgtat tcgactcaac ctccggttag
     4021 ccgccattaa actgttactt gattctagca aaggtttcag tttggctcaa ggaatgaaat
     4081 gggagattac cgtctctttt ggcagatcga acaaaagaac gagctgcgtt cactctgcag
     4141 gcgtgtgatc agagtggggc atccatgtaa gcaaggcaat cctgccttct gatatatctg
     4201 gtgcgcttgc tggaaccgtc cgtacgaccc atccggagca cggaaatctc tgatggaaga
     4261 ccctccagcg gcaatggctt cacctaatac ggtatgcatg gtgtcataga gccgttgaca
     4321 ggctgagacg cccagccgat agcctcgagc ttgtgggtgc actctggccc gaaacaggat
     4381 ttcattggcg taaatatttc caatccccgc gagccgatgt tgatcgagaa gaaagggttt
     4441 cagcctgcct cgacagcttt gaatgagctg gataaaggat tgtgcactta tatccatggc
     4501 gtcagcaccg aatcggcgat gcatgaaggc ttccaattcc ggtagggtga acagccagag
     4561 ccggccaaac cttcgaggat tccagtaatg tagttcagtg gccggaactc cggaaatctc
     4621 gatgcggcaa tggatgtgtt gaggggttga ggccaaggcg ggattaaaaa accagagtcc
     4681 ggtcattccc tgctcggaaa gcagataccg ggtttcgttg gctcgatggc agctaaagat
     4741 gagacatttg cccttccggg ctatatgggt gatcgtggaa cgctgaaacc aggggatgag
     4801 gtcgtgtccg gtgcgcacaa tatccgaacg gtgtatggtg agcattgcaa tggtcccacc
     4861 caggacatgc cggttcagat ggttgaggat gacctggact tcggggagct cgggcataga
     4921 gggagccgcg gggaaaatga aagggttgag cgggcgtgga tcgttcttcc ttatggtctg
     4981 gaaggttgta tcagatttga agtggcgctg ggtatcgcca tgtttacttt ttttgtgagg
     5041 tatcctcatg ggtaagccaa tgaactccgg ctcaggtgtg ataagattga agcaagccag
     5101 gccctggatg ccggacctgc tgtgtaaaga ggataaggag catttggttg cgggatctcg
     5161 gggaggaagc ctatacttta gagaggaact tgctcaatag cgtggattaa ggaagaagca
     5221 tgacacaatt aagc
//
LOCUS       DATIAL010000538         2657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1062668, whole genome shotgun sequence.
ACCESSION   DATIAL010000538 DATIAL010000000
VERSION     DATIAL010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062668"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VLA60_16495"
     CDS             <1..922
                     /locus_tag="VLA60_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HSF11012.1"
                     /translation="EASDEYGYQKMWIFLNDRFVKKEQARISVFDHGFLYGDGVYETL
                     RTYQRRIFLLERHLARLRHSCNLIGLVIPITDDAWLSIFTEILNRNSLEDASLRLTIS
                     RGEGELGIDPGLCASPTVVVMARSFVPYPPRMREEGIRLQLVSVRRNPISAQSPQIKS
                     LSFLNNILAKQEAVRAGAYDALMLNMDSQVTECTTSNIFFVINQRLHTPSVDCGILEG
                     ITRAVVIEIARNLGIEVKEGAYTAEELLQAEECFMTNTSLEVMPVSQIGEHVIGQGKS
                     GPITMRLWKAFQQNLERFLGPRVAVPQASI"
     gene            complement(907..2571)
                     /gene="nadB"
                     /locus_tag="VLA60_16500"
     CDS             complement(907..2571)
                     /gene="nadB"
                     /locus_tag="VLA60_16500"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HSF11013.1"
                     /translation="MASSATSRVSPQVISTDFLILGAGVAGLRAAIELSHYGRVTMVA
                     KGGPQDNNSFYAQGGVAVALSEEDDVILHFEDTLKAGHQLCSRPATKVLVEEGPSRIH
                     ELIEWGATFDTVDGKLAFTMEGAHSRHRVLRAGGDATGSEMVRALSVKAQSLKNLTWM
                     GNHFAVELCIQDGRCWGASILDELSGTVKIILASATLLVTGGAGQVYARTTNPANATG
                     DGIAMAFRAGVILEDMEFVQFHPTALYLPSSPPFLLSEALRGEGGILRNNRCEPFMKH
                     YHRSLELAPRDIVSRAIWTEMQRTKARHVYLDVTHLGATFLKERFPTIYATCLRYDID
                     ITEEWIPVSPSAHYFMGGVKTNLHGASNLPGLFAAGEVACSGVHGANRLASNSLLEGL
                     VFGYRAARAAAEFLPSGFPPTCTGITLFPKSNARTMLTQDVEKIRNSLRRLMWAKVGL
                     VRTGESLKKAVDQIQQWSQKLSAAPCNRPGLETRNMAQVGQCIAEAALWRANSVGAHF
                     REDFPFYKGLEWKTHSRCQQGVSNGSVVRIRKKSKKHLSALNQIEA"
BASE COUNT          669 a          657 c          668 g          663 t
ORIGIN      
        1 ggaagcttcc gatgagtatg ggtatcaaaa gatgtggata tttttaaacg atcggtttgt
       61 taaaaaagag caggctcgga tttccgtttt tgatcacgga tttttatatg gtgacggggt
      121 ctatgaaact ctgcgtacct accagaggcg gattttttta ttagaacggc atctggcccg
      181 tctccggcat tcatgtaatt tgatcggctt ggtaattcca atcacggatg acgcctggct
      241 gtccattttc accgaaatcc tgaaccggaa tagccttgag gatgcaagcc tccgtctcac
      301 gatttctcgt ggagaaggag aactcgggat tgatcccggt ctttgtgcca gcccaacggt
      361 cgtagtgatg gcgaggtcat ttgtccctta tccccctcgc atgcgtgaag aaggtattcg
      421 tttgcagctc gtctcagttc gacggaatcc aatttctgct caatctcctc aaattaaatc
      481 cctgagtttt ctcaacaaca tcctggccaa acaggaagcc gttcgggcgg gagcctatga
      541 tgccctgatg ttgaatatgg atagtcaggt gacggaatgc acgacaagca atatcttttt
      601 tgtgatcaat cagcgtttgc ataccccgtc cgttgactgc ggcatcctgg aggggatcac
      661 cagggcagtg gtaatcgaaa tagccaggaa tttaggtatt gaagtaaaag aaggtgcgta
      721 tacggctgag gaattgcttc aagcagaaga gtgttttatg accaacacga gtctggaagt
      781 catgccggtt tcgcagattg gagaacatgt gattggccag ggaaagagcg gaccgataac
      841 gatgaggctc tggaaggctt ttcaacagaa tttggaacga tttttgggcc cccgtgtggc
      901 agtgcctcaa gcctctattt gattcaacgc ggagaggtgt ttcttcgatt ttttccggat
      961 gcggacaacg gatccgttcg acacaccttg ttgacaccgg ctgtgtgttt tccattccaa
     1021 tcctttgtaa aacggaaaat cttcacggaa atgagctccc acgctattgg ctcgccaaag
     1081 cgcggcttct gcgatgcatt ggccgacctg ggccatgttc cgggtttcta atcctggccg
     1141 gttgcaagga gcagccgaga gtttttgtga ccactgctga atttgatcga ccgctttttt
     1201 taatgattcc ccggtgcgca ccaatccgac tttagcccac atgaggcgcc gtagggaatt
     1261 ccggattttt tccacatcct gggtgagcat ggtacgggca ttggatttcg gaaaaagagt
     1321 gatgccggta caagtaggag gaaaaccgga tggcaaaaat tcagccgccg ctcgggctgc
     1381 gcgatagcca aatactaacc cttccaatag cgaattactg gccaatcgat ttgccccatg
     1441 gactccggaa cacgccacct ctcccgcagc aaagagtccc ggaaggttgg atgccccatg
     1501 caggttggtt ttgaccccgc ccataaaata atgggcgctg ggggagacag gaatccattc
     1561 ctcggttatg tcgatgtcat accgcaaaca ggttgcatag attgtgggga aacgttcttt
     1621 caaaaatgtt gcccctaaat gagtgacatc caaataaaca tgtctagctt tggtgcgctg
     1681 catttccgtc cagatggccc gggaaacgat atcccggggt gcaagctcca ggcttcgatg
     1741 atagtgtttc ataaaaggtt cgcagcgatt attgcgcaag attcctcctt ccccccgcaa
     1801 agcttctgaa aggagaaagg gaggacttga gggaagatac agggccgtcg gatgaaattg
     1861 aacgaattcc atatcttcca ggatcacccc ggcacgaaaa gccatggcaa tgccatctcc
     1921 ggtcgcattg gccggattgg tggttctggc atatacttgc ccggctcccc ccgtgactaa
     1981 caaggttgcc gaagccagga taattttaac ggttccactg agctcatcca gaatcgatgc
     2041 tccccaacat cgtccgtctt gaatacacaa ctctactgca aaatgatttc ccatccaggt
     2101 cagattttta agactttgtg ctttgacgct taaggcgcga accatttcac tcccggtggc
     2161 atctcccccg gcccgtaaca ccctgtgtcg actatgggct ccctccatgg tgaacgccag
     2221 tttgccatcc actgtatcga atgtggcacc ccattcaatc aattcatgaa tgcgtgaagg
     2281 gccctcttcc accaacactt tcgtggccgg gcgagaacaa agttgatggc ccgccttcag
     2341 agtatcttcg aaatgaagaa tgacgtcatc ctcctcactc agagccacgg ctacgccacc
     2401 ttgtgcataa aaagaattat tatcttgagg accaccctta gccaccatgg tgactcgtcc
     2461 ataatgactt aattcgattg ccgcccgaag gcctgctacc cctgcgccta aaatcaggaa
     2521 atcagtggaa atgacttgag gggaaacgcg tgaagtggca gaagaagcca taacaataaa
     2581 aaggattgaa ccaaaataaa ttgtcaaatt aagaggaaaa gtttggacga tgcgtcatgc
     2641 caaagggcag gtctcct
//
LOCUS       DATIAL010000539         4738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_420349, whole genome shotgun sequence.
ACCESSION   DATIAL010000539 DATIAL010000000
VERSION     DATIAL010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420349"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VLA60_16505"
     CDS             complement(<1..1001)
                     /locus_tag="VLA60_16505"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HSF11014.1"
                     /translation="MTRKIPSLSFATGHTAGLAFFLLGLLTNPAFSASSIQLTWNANS
                     EPDLMGYYVHMGTSPSSYNTRENAGLVPSYTFKNLPEGITYYFTVTAYDQSGNVSEPA
                     KEVSIALPIHNTNPSPPRSQDLNGDGKADLVWHNTKTGQVAVWLLNGTSVTTSGFLGQ
                     LPTEWALSGVDDLNGDDKADVLWRNDTNGAVAAWLMNGLTITSTGFPGSASTAWAIQG
                     IGDVNGDGKADLIWRNTTDGNTAIWIMNGAAIAATGFPAGVPLEWQIAGVGDVNGDGK
                     ADVIWRHDTSGTMAIWLMNGLTISSTAFPGSAPTTWTIQAVGDVNGDGKADLVWHNIT
                     "
     gene            1627..1782
                     /locus_tag="VLA60_16510"
     CDS             1627..1782
                     /locus_tag="VLA60_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11015.1"
                     /translation="MVLRRALVRGLQPYKARVHILTYYEGLEFSEQVGIAQDLEAQIY
                     FARPYGS"
     gene            complement(2211..2900)
                     /locus_tag="VLA60_16515"
     CDS             complement(2211..2900)
                     /locus_tag="VLA60_16515"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HSF11016.1"
                     /translation="MKKRILAGFVAGCLLWAPAFCFGGNEPIQDFRLWAPVYLNFPIS
                     GPVKGYMEANPRFSDDASQIDQLLLRPAVGYQLTSTFSLWQGYAWVANYEPRYLQEHR
                     IFQQLIYSNKFSTFKLLSRSRLEERWIQNAIGTAVRARTMLRGDFPLPAYPALALAAY
                     DEIFVNLNTIRQGPEAGFDQNRFFLGLNYTTSPNLNVDVGYQLQLINTPLSGLANQTN
                     HILLIQFFINL"
     gene            complement(2958..3119)
                     /locus_tag="VLA60_16520"
     CDS             complement(2958..3119)
                     /locus_tag="VLA60_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11017.1"
                     /translation="MMRVILVWLVASIGLAAPLFAWEGPQVQYSANTRMETADGVMEG
                     MVFHNFDHS"
     gene            3522..4628
                     /locus_tag="VLA60_16525"
     CDS             3522..4628
                     /locus_tag="VLA60_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HSF11018.1"
                     /translation="MPKICILGGGKIGSLIATLLVECGDFDVVLGDVDPEVVARLKQD
                     IGHDHFQVSSVDVQDAKALGRFIDSVGPEGIISCLPYFCNQAVGEQARACGAHYFDLT
                     EDVEVTRKIREVSEGASSAFVPQCGLAPGFISIVAHELMSRFDTVDMVKMRVGALPVN
                     PSNVLKYSLTWSTDGLINEYGNTCYGIENSREVPLLPLEGYETISIDGLLYEAFNTSG
                     GLGTLADTYAGKVRTMNYKTLRYPGHCEKIHLLMNDLKLNDDRETLKRILEKAVPKTL
                     QDVVLIYTSVTGLRKGELYEENFVQKIYPQTIAKKLWAAIQVTTAAGMCSVVDLVFER
                     PGKYQGFVTQETFDLPSILANRFGRFFQPDDSCS"
BASE COUNT         1128 a         1037 c         1337 g         1236 t
ORIGIN      
        1 gtgatgttgt gccagacgag atcagctttg ccatcgccgt tgacatcccc cacggcttgt
       61 atggtccaag tcgtcggcgc actgccggga aaggctgtgg agctgatggt caatccgttc
      121 atcagccaga tggccatcgt accgctggtg tcatgacgcc aaatgacatc ggcctttcca
      181 tctccattta catcgcccac tcccgcgatc tgccactcta aggggacgcc agcagggaat
      241 cccgtggcag caatcgctgc gccgttcatg atccatatag cggtgttgcc atcggtggtg
      301 ttgcgccata tcagatcggc tttgccatcc ccgttaacat ccccaatacc ctgaatggcc
      361 caagctgtcg aagcgctgcc gggaaaacca gtggaggtga tggtcaaccc gttcattagc
      421 catgcggcca ccgcaccgtt ggtgtcattg cgccagagga catccgcttt gtcgtcccca
      481 ttcagatcgt ccactccact taaggcccat tccgtgggca gctggcccag aaaacctgaa
      541 gttgtgacgc ttgtgccatt gagtagccat actgccactt gccctgtttt cgtgttgtgc
      601 cagacgagat cggccttgcc gtccccattc aagtcctgac tcctgggagg actgggattc
      661 gtgttatgaa ttggaagggc tatcgatact tccttcgcgg gttcactgac atttcctgat
      721 tgatcatatg ctgtgactgt aaaataatag gtgattcctt ccggaaggtt cttaaacgtg
      781 tacgatggga cgagcccggc attttccctg gtgttgtaag agcttgggga tgtgcccata
      841 tgcacataat accccatcag gtccggttct gaatttgcgt tccaggttag ttgaatggag
      901 gaggctgaga aagcggggtt agtcaataat ccaaggagaa aaaaggcaag gccagccgta
      961 tgcccggttg caaagctcaa ggagggtatt tttcgagtca tactctattc ctgctggcgt
     1021 tcggactgca gtccaaattc tttccaaaag gcttaccccc tccttgggtg gctgaatgaa
     1081 attaggtttg ctcacctgac ttctggtttt aaggctctac tttataataa cctggcctgt
     1141 tttgaaaaga tgtttttatg aattaataag cgttggctaa gaatttctgt gaatttttat
     1201 ccttagatct aaatacacga gctgtcgact aattcagaga agtcagtaaa aattcaatag
     1261 gctgggattc ttggttttgg tcaagcaatg gttccagaaa aaagtaaatt gaatggtggc
     1321 aaattttata ggaaggtatc tttaagagaa tataggccac aatcagtcga gaactccgac
     1381 ggaatcatgg gtggcgaagc aatcgaccgc atcaagccat tgttgggatc aagcacgaca
     1441 attgactcaa cgcccccctc gttgctccgg cgccccgtgg cagccgatcg cccctttgat
     1501 tcgccagggt tggagtctcg agcaaatccg tggacggttg cagcataaac aagattggac
     1561 gattagtcct gaatagatct atcagtatat ctctgaggat caacgggcgg gcggtataaa
     1621 acagcggtgg tccttcggcg ggcccttgtc cgtggcctgc aaccgtataa agcccgagtg
     1681 cacatcctca cgtattacga gggtcttgaa ttcagtgaac aggtaggcat cgcgcaggat
     1741 ctggaggcgc aaatctattt tgcccgtccg tatggctcct aggagcaagg cgtgaacgag
     1801 aatgccagtg ggcttatccg ttggtctttc cccaaagacc gtgattggtc tcaagtgggc
     1861 tgaattacaa gaagtaagcg acaggctcaa tcatcgaccg agaaaggccc ctgggttatt
     1921 aagcgccgca tgagatattc tttgaaatcc caacatcatt aactgttgca cttgcgactt
     1981 gaatccaaga gatgaagggg gtgagaaatt tttataaacc ccttcggaca attgtcagag
     2041 gactcagttg aaggtctttg tgcagaggct gtgctggata atcacatccc tagattaaaa
     2101 tttctgttat gtcttgcctg ttgatcgaaa atctacttta gatgttattg attatgagat
     2161 tgggacacga tcattgcgta acaatcttcc taatgcagcg atggaaaatt ttatagattg
     2221 atgaagaatt gaatgaggag aatatggttg gtctgattgg ccagacccga gaggggcgtg
     2281 ttgatgagct gtaattgata gcccacatca acattgaggt tgggggaggt cgtatagttg
     2341 agacccagaa aaaaccggtt ttgatcaaat ccggcttccg gtccctgacg gatggtattg
     2401 agattgacga aaatttcgtc ataggcggca agagccagcg cgggataggc gggtagtggg
     2461 aagtctccgc gaagcatggt tcgagcgcga accgctgtcc cgatggcgtt ttggatccat
     2521 cgttcctcga gacgggagcg gctgagcaat ttaaaggttg aaaatttgtt gctatagatg
     2581 agttgttgga aaatccgatg ctcctgtaag tagcgtggtt catagttggc cacccaggcg
     2641 tacccctgcc agagtgagaa ggtggatgtg agttgatacc ccactgccgg acggagaagg
     2701 agttggtcga tttgggaggc atcgtccgag aatcggggat tggcttccat gtaccctttg
     2761 accggtccgg aaattggaaa gttgagataa accggtgccc acagtcgaaa atcctgaatc
     2821 ggctcgttgc ctccaaagca gaaggcgggt gcccacagta ggcatccggc aacgaagcct
     2881 gccagtattc ttttcttcat tattattaca ttcaccctgt tccccacctc attgtaagac
     2941 tcatccttcg tgctttccta ggaatgatcg aaattgtgaa agaccatgcc ttccatgact
     3001 ccatccgccg tttccattcg ggtattggcg gaatactgga cctgcgggcc ttcccatgcg
     3061 aagaggggag ctgccagtcc gatacttgct acaagccaca cgaggattac acgcatcacg
     3121 agattctcct tgttgttaga ggatgagtgc cgatgcactc tcgtcgaata tgtgggaatg
     3181 ttgcccctag ttgaaatggt ggatggctga agtgtggagg tgtttgggga gctcatgttt
     3241 taggaaagca cgccttcggg agggaaacgc tactctttcc tgaaaatcct gtcaattgga
     3301 agtttagcca gtgaatctta gaacgattgg tcggatctgg catcgacttt tccggtgttt
     3361 tggcggatga atagggactg gaaggtaccg gccccggatg attggaaagg cttgaccgaa
     3421 gagattcatc tattcttttt tcttgggttc gagtctactc tcatgggtgt caaaaagggt
     3481 aaaatatgtc aacaaaggga ttttttaaaa aagggtacgg tatgccaaaa atttgtattc
     3541 tcggaggagg gaaaatcggg tcgctgatag cgacgttatt ggtggagtgt ggagactttg
     3601 acgtggtgct gggagacgtc gatccggaag tggttgccag gctgaagcag gatatcggcc
     3661 atgaccattt ccaggtgtcc tctgtcgatg tccaggatgc caaagccctg ggtcgattta
     3721 tcgattctgt cggaccggaa gggattattt cctgtcttcc gtatttttgc aaccaggcgg
     3781 tcggagagca agctcgagcg tgtggggcgc actattttga tttaaccgag gatgtggaag
     3841 tcacgaggaa gattcgtgag gtgagcgagg gagcgtcctc ggcttttgtg ccgcaatgcg
     3901 ggttggcgcc gggctttatc agcattgtcg cgcatgagct catgagccgg tttgatacgg
     3961 tggatatggt gaaaatgcgg gtcggggcgt tgccggtgaa ccccagcaat gtgttgaaat
     4021 attcgctgac gtggtccacg gatggcttga taaatgaata cggcaatacg tgctatggga
     4081 ttgagaatag ccgggaagtg ccgttgttgc ctttggaggg atacgaaacc atttcgattg
     4141 acggcctgtt atatgaggcc tttaatacct ctggtgggct ggggacgttg gcggatacct
     4201 atgccggcaa ggtccggacg atgaattata agacgctgcg ctatcccggc cattgtgaaa
     4261 aaattcatct gttgatgaat gacctcaaac tcaatgatga tcgagagacc ttgaagcgta
     4321 ttttggaaaa ggccgtgccg aagactttgc aggatgtggt cctgatctat acgtcggtga
     4381 cgggtcttcg gaaaggcgag ctgtatgaag aaaattttgt gcagaaaatt tatccacaga
     4441 cgattgccaa aaaattgtgg gctgcgattc aggtgacgac agcggccggg atgtgcagtg
     4501 tggtggatct ggtctttgag cgtcctggaa aatatcaggg gtttgtgacg caagagacat
     4561 ttgatctgcc ctcaatcctg gctaaccggt tcgggcggtt ttttcaaccg gatgattcgt
     4621 gttcgtgatg agcctatggt gtgtcaaagg aaatggagcg cgtgatggat gttttccatg
     4681 aattggggtt aaaagcagtg aatgccggcg cgtgttccgg ccaggggcgt tggatgtc
//
LOCUS       DATIAL010000540         3920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1170906, whole genome shotgun sequence.
ACCESSION   DATIAL010000540 DATIAL010000000
VERSION     DATIAL010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170906"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2396)
                     /locus_tag="VLA60_16530"
     CDS             complement(<1..2396)
                     /locus_tag="VLA60_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HSF11019.1"
                     /translation="MLRLNTFCVLAMVLMMLLISCTLRETQLGDELEQKGDFDGAIAA
                     YREALKKDPFNKEIDDKFKAVKIRAADQHFSRGRQMLKERKMGEALQEFQIAVGLDPQ
                     NKEHHTALNDVWRLKSAHQTFLDANNMEGLGRYEEAMALYESAVELDPSLSEAVEGIT
                     RVVQLEKATQAIGGSAEPVTLRFQNTRLKQVFEILARTANIDILFDKDVRDDLVTIFT
                     KDTPFDEALNLILTTNQLFAKRVGPDRLLVIPDTKQKREQYDDLQIRTFYLSNAKAKD
                     VANLLRTILETKRVYVNEPLNTVVIRDEPAKVTLAEQIILANDRSDSEVLFEVEVLEV
                     NRTNTQNLGLQFAKEAGFGIFPPNFDSKDPTTPLKGTFNTLASSWTFQELTSIGQSSY
                     LFRFPSSVLLNFFKQESQAKTLASPKLRVLNNQKASINVGDKQPILLSTTNVLPGQAA
                     TGAVPTTSTVTSIEFKDTGIKLTVEPTVHLNHTISLRLQIEVTRLGERVILQASPLIT
                     QFKFGTRTADTALNMRDGETVVLGGLLAEDNTKTRESVPGVDDIPVLGDIFSSKNTQK
                     VVTEVILVITPHIVRTVQPQQLTKQTLWSGTANQYTTKPMFSQPNPAVSLLNTQDLPP
                     DMTDFSESAAFPKDSKLTEGKTPISTSVEPGSADASQPVTDGPRLQVLPTAASAKVGD
                     QISFTIQGQNLSSVGKTSLTLTYDPAVLSFMQANEGTVWTSQQIVPSMTVSAVPHLGQ
                     LVLQMGQEGTVVQGSGSLATVAFEATGPGTSSIHIQQSTFLGANGQSIPVMVEHGRVL
                     V"
     gene            complement(2725..3366)
                     /locus_tag="VLA60_16535"
     CDS             complement(2725..3366)
                     /locus_tag="VLA60_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11020.1"
                     /translation="MRKSFREYSESRMVSLTNQNGVTYLFVMMLVVVMSIATLEVTQP
                     WSVIMQRDREAELQFRGTSIAKAIERFVADWEIQKATRPNQWPRTLEELTKKSPKRYL
                     RKAYADPMTGNKFALIKIGQEIHGVRSTSTETPYNQDIFKGAKTYQAIRFEAKGSTNC
                     QVNPLNPNAPTNCAPVANTLPSGETSLGDTPSATSPVQAPSPEAPPEETHTFQ"
     gene            complement(3441..3830)
                     /locus_tag="VLA60_16540"
     CDS             complement(3441..3830)
                     /locus_tag="VLA60_16540"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HSF11021.1"
                     /translation="MILKYWGPVVLYALAIVYLSSQSNPAQQLHLPSFIFNINDKFLH
                     ACEYGVLGILLYRALNYTTPNAGNMGLAIICVIAFGISDEIHQWFVPQRQADILDLVA
                     DTFGAAFLIFGWVLITEKGRRRLTIRK"
BASE COUNT          929 a          865 c         1040 g         1086 t
ORIGIN      
        1 accaacactc tgccatgttc caccatgacc gggatagact ggccattggc tcccaagaac
       61 gttgattgtt gaatgtgaat gcttgaggtc cccggccccg tggcttcaaa agccaccgtg
      121 gcaagtgagc cgcttccttg gacgactgtt ccctcctggc ccatctgaag aaccagttgt
      181 cctaaatgtg gcacggctga aacggtcata cttggaacta tctgctgaga tgtccatact
      241 gttccttcat tggcctgcat aaaggagaga actgctggat cataggtcag cgtcagagag
      301 gtttttccga ccgatgacag gttttgccct tgaatggtga aggatatttg gtctccaact
      361 tttgctgacg ctgcggtcgg cagtacttgc aggcgaggcc catcggtcac aggctgcgat
      421 gcatccgccg agcctggttc tacggatgtt gaaatgggtg tctttccctc agttaatttg
      481 gaatctttgg ggaacgctgc cgattcggaa aaatcggtca tgtcaggggg caagtcctga
      541 gtattcagaa gagacacagc cgggttgggt tgtgaaaaca tcggcttggt ggtgtactga
      601 ttggccgtgc cggaccagag agtctgtttc gtcaattgct gaggttggac ggttctgacg
      661 atatggggag taatcaccaa aatgacctcg gtcacaactt tttgggtatt tttgctactg
      721 aaaatgtcac caaggactgg aatgtcatcg acaccgggaa cggactcccg cgtctttgtg
      781 ttgtcctctg caaggagccc gccaagcacg acggtctcgc catcccgcat attgagtgcc
      841 gtgtccgcgg tacgggttcc aaacttgaat tgggtaatta agggagaagc ttgaagaatg
      901 acccgttcgc cgaggcgtgt gacttctatt tgcaatcgta gggaaatggt gtggtttaaa
      961 tgcacggtcg gctcaacggt gagtttgatg cccgtatcct taaactcaat ggaggttacg
     1021 gtcgaagtcg tgggtacggc acccgtggcg gcttgacccg gtaacacatt ggtggtggaa
     1081 agaagaattg gttgtttgtc cccaacattg atggaggctt tttgattatt gagtaccctt
     1141 agcttcggag aggcaagggt tttagcctgg gattcctgtt tgaaaaaatt cagtaacaca
     1201 ctgcttggaa aacgaaacag atacgagctt tgaccaatgc tcgtgagttc ttggaatgtc
     1261 catgatgatg caagtgtgtt aaaggttcct tttaatggtg tagtaggatc ctttgaatcg
     1321 aaatttggtg gaaaaatacc aaatccagcc tctttggcaa actgtaaacc cagattttgg
     1381 gtgttggtac ggttgacttc caagacttcc acttcaaaca ggacttccga gtcgctacga
     1441 tcattggcca ggataatctg ctccgccaga gtgaccttgg ccggttcgtc ccttatgaca
     1501 acggtgttta atggctcgtt cacatagacg cgcttggttt ccagaatcgt ccgaagaaga
     1561 ttagcgacat ctttggcttt ggcattggag agataaaatg tgcggatttg caggtcatca
     1621 tattgctctc gtttttgttt ggtatccggg atgaccagga gccgatcagg gcccacgcgt
     1681 ttggcaaaca gttgattggt ggtcaggata agattcaggg cttcatcaaa cggcgtgtct
     1741 ttggtgaaga tcgtcacgag gtcatcccgc acatccttat cgaataaaat atcgatgttg
     1801 gcggttctcg ctaaaatttc aaagacttgc ttgagacggg tattctgaaa ccgaagagtc
     1861 acgggttctg ccgaaccacc gatggcttgc gtggctttct ccagttggac cactctcgtg
     1921 atgccttcaa cagcctccga cagagatggg tctaattcca cggctgactc atagagcgcc
     1981 atggcttctt cgtatcggcc aaggccctcc atgttgttgg catccaaaaa ggtttgatga
     2041 gcagacttta agcgccagac atcgttgagg gccgtatggt gttctttatt ttgtggatcc
     2101 agtcccacag ctatttgaaa ttcctgaagg gcttcgccca ttttccgctc tttcaacatt
     2161 tgtcttccac gggaaaaatg ttgatctgcg gcacgtattt tcaccgcttt gaatttatca
     2221 tcaatttctt tattaaaggg gtcttttttt agggcttccc gataagccgc tatggcccca
     2281 tcaaaatccc ccttttgttc aagttcatcc ccaagctgtg tttcacgaag agtgcaggag
     2341 atcaaaagca tcatcaagac catggccaac acgcaaaacg tattcaatct aagcattaga
     2401 accttcgcta tatttaatga gaatgacggg ggggtaagac agaagaaatc aaaaatcctg
     2461 ttgaacatat catatctgtc aagttcgagc tagagatccc tgctcgtttc tttaatctac
     2521 tctagtttct gtatcggata tcacttcgtc aatattgagc ctggaaagtt ttgtcagaaa
     2581 atacacggga tggttggtta ggcattaacg agtcggaaaa tgcttaccct ccttaggtgg
     2641 ttgcagctca ttgaaggccc tgaagcacgc acgaatagga gcattatcac acatgatcgt
     2701 cctggagaat ctagtggttt aatcttactg aaaagtgtgg gtttcctccg gtggggcttc
     2761 tggtgatgga gcttgaacgg gggaagttgc cgaaggagta tcccctaggg aagtttcccc
     2821 ggaaggaaga gtgttggcta cgggagcaca gttggtcggc gcattaggat tgagcgggtt
     2881 gacctggcag ttggtgctgc ccttggcttc gaagcggatg gcttggtacg tcttggcacc
     2941 cttgaaaata tcctggttgt atggggtctc ggtgctggta cttctgactc catgaatctc
     3001 ctggccaatt tttataagcg caaacttgtt tccggtcatg ggatcggcgt aggccttccg
     3061 gagataccgt ttgggtgact tcttcgttaa ttcttcaagc gttcgtggcc attggttggg
     3121 tcgtgtggct ttttgaattt cccagtccgc cacaaatcgc tcaatggctt tcgcaatact
     3181 ggttccacga aattgtagct cagcctctcg atcccgttgc ataatgaccg accacggttg
     3241 agtgacttcc aaggttgcaa tggacatgac gacaaccagc atcatgacga aaaggtaggt
     3301 taccccattc tggttggtga gagagaccat ccggctttcc gaatattccc tgaatgattt
     3361 tctcattgag tgatgctgtc tgcgtgttgg ggtgcaggca tcagtttcgt gacggactgt
     3421 gccatggggt tgagtgagtg ttacttgcgg atagtcagcc gtcttcgtcc tttctctgtt
     3481 atgagcaccc aaccaaaaat taaaaatgcg gcgccaaaag tatcggcaac tagatccaaa
     3541 atgtcagcct gacgctgagg gacaaaccat tgatggatct catcggaaat cccgaatgcg
     3601 atgacgcaga tgatcgcgag gcccatattt cccgcgttcg gggttgtata attcaatgcg
     3661 cgatagagca gaatcccgag tacgccatat tcgcaggcat gtaaaaattt atcattaata
     3721 ttgaatataa aggaagggag gtggagttgt tgggcaggat tggattgaga ggagagatac
     3781 acaatagcga gcgcatagag gaccactggt ccccaatact tcaatatcac gaatggtttt
     3841 gctatggttt gggtcggaga aatctgacac gtcctttttt aatggtggct taattgttgg
     3901 atgttaatcg gtttggccgg
//
LOCUS       DATIAL010000541         4128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1386181, whole genome shotgun sequence.
ACCESSION   DATIAL010000541 DATIAL010000000
VERSION     DATIAL010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386181"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1727
                     /locus_tag="VLA60_16545"
     CDS             <1..1727
                     /locus_tag="VLA60_16545"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HSF11022.1"
                     /translation="ALLLSTVLGFVPSEEAFLGFGHPAVVTVAAVLIISRGLSNAGVV
                     EILTRYVTGATSFSPSLHVAVLSSLGGLISTVMNNVGALALLMPVAIQSSVEAKRSPA
                     VVLMPLAFGTILGGMVTLIGTPPNIIVAHYRAEVAGEPFGMFDFTPVGGIMALVGIIF
                     VALVGWRLIPIARRSQNVPQDLFSIQEYLTEVEVPENSKAIGKSLSELELATEDSDAL
                     IIGLVRGDQSIRGAAWRERIQVGDVLVLEAGPQGINKFVSTLGLKLTGTQPNEEVQES
                     GKKPSAERDGDMLMEAVIPPDRSWLVGRMAGSLKLRSRFGINLLGASRQGTPYRGRLR
                     EFRFKAGDVLLLQGEKERVLEVIGLLGCLPLAERGLQLGKPGQAWLAGGLFALALGSA
                     LAGMTSLPIALIGAALGMVLLGIVSPRDVYHTVDWPVLVLLGAMIPIGQALEQSGATA
                     VVAESLVTMTEGVAPIMLLGLIMLITMTLSDVMNNAATAVVMAPLSVAVAHHLGLNAD
                     PFLMAVAIGASCAFLTPIGHQNNLLVMGPGGYRFGDYWRMGLPLELLILVVSLPLLVF
                     MWPLTGQG"
     gene            1829..2833
                     /locus_tag="VLA60_16550"
     CDS             1829..2833
                     /locus_tag="VLA60_16550"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HSF11023.1"
                     /translation="MPTDLDPFRTLLSRIILVVMLFCFLTGILWLDRNGLKDQIDGEI
                     SFSDVIYFTMVTVTTVGYGDIVPVSTRARLIDAFVVTPVRIFLWVIFLGTAYQLAFRQ
                     FTEVFRMAKLQRSLDQHVVICGFGHTGYSTVKELLAKGTNPDHILVIDPGEERVRIAG
                     ELGVVALRGDATQEGLLKYGAFLNEAKAVIISAGRDDTNALILLTVRHLNSRCRVIVS
                     AKEEENVKLFRQGGAQTIISPATYGGYMLAAAVDQQYLADYLEDFLTAGGKVNIEERT
                     VDKQDIGKSAMDLQPDVLLRVYRQGSILSPWEFQKNHRLEQGDVMLLFKPVSEDMPSS
                     "
     gene            complement(2852..3622)
                     /locus_tag="VLA60_16555"
     CDS             complement(2852..3622)
                     /locus_tag="VLA60_16555"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HSF11024.1"
                     /translation="MAFLSGGIVGVLLGATGGGGSLVAIPLLVYVVGVPVQNATTMSL
                     IVVGYSALFGAWHESRQQRVHFVSAILFSLTGMIGAWAGAHGHQLVPDSLVLFAFGNL
                     LLFISMWTCWKNYEGEHNDVPSGCAKEFSWRCAWKALIFGWGVGLLTGFFGVGGGFLI
                     VPALMFLMGFPIRLAIGTSLLIIALISIGGVIGHFEGARLDMVLTGLLLLGSLLGLLF
                     GSQVTQSIPAHHLRRGVAILIGVIGILLIMANARPLLF"
BASE COUNT         1032 a          964 c         1103 g         1029 t
ORIGIN      
        1 gtgctttgct tctttcaacc gtgttggggt tcgttccctc cgaagaagca tttttaggat
       61 ttggtcatcc tgccgtggtc accgtggcgg ctgtgttaat tatcagtcgg ggattgagta
      121 acgcgggagt cgtggaaatt cttacgcgct atgtcacggg tgcaacgtct ttttccccgt
      181 ccctccatgt ggcggtctta tcttctctcg gtggattgat ttcaaccgtc atgaataatg
      241 tgggagccct ggcgttactg atgccggtgg ctattcaatc gtccgttgag gccaagcggt
      301 ccccggccgt ggtgttgatg ccattggctt ttggaacgat tttgggcggt atggtgactc
      361 ttattgggac acctcccaat atcattgtgg cccattaccg cgcagaagtc gccggcgagc
      421 cattcgggat gttcgacttt actcctgtag gaggaatcat ggcgctagtg ggaattatct
      481 ttgtggcgct agtgggttgg cgtcttattc ccattgctcg acggtctcaa aatgtgccgc
      541 aagacctgtt ttcaatacag gaatatttaa cagaggttga agtgcctgaa aattctaagg
      601 caataggaaa atccctgtct gaactggaat tggccactga ggattctgac gccttaatta
      661 ttgggttggt gcggggtgac caatctattc gtggggcggc ttggagggaa cgcattcagg
      721 tcggggatgt ccttgtcctc gaagcggggc cccaaggaat taataaattt gtgtccacat
      781 tggggttgaa gttaacagga acccagccta atgaggaggt tcaggagtct ggaaaaaagc
      841 catccgcaga aagggatgga gatatgctga tggaggccgt cattcctcct gatcgttctt
      901 ggttggtcgg gcggatggcg ggctctttaa aactgcgaag ccggttcgga attaacttgt
      961 taggagcatc tcgtcaaggc actccttacc gtggcaggct gagggaattt cggttcaaag
     1021 ccggagatgt gcttctgctg caaggagaaa aagaacgggt cttagaggtt atcggactgc
     1081 tcgggtgtct ccctttggcc gagcgaggcc ttcagttggg aaagcccgga caggcatggc
     1141 tggctggagg gctctttgct ctggccttag gaagcgcctt agctggcatg acatcactac
     1201 cgattgcgtt gatcggtgcc gccttgggaa tggtgctgct gggtatcgtg tcccccagag
     1261 atgtctacca taccgtcgac tggcctgtcc ttgtattatt gggggcgatg attcccatag
     1321 gacaggcgtt ggaacaaagt ggcgcaacgg cggtagtggc ggagtctttg gtcactatga
     1381 ctgaaggagt tgccccaatc atgctcttgg gactcatcat gctcatcacc atgacgctgt
     1441 cagacgtcat gaacaatgcg gccacggcgg tggtgatggc acctttgagc gtggcagtgg
     1501 ctcaccatct tggtctcaat gcggacccct ttctcatggc ggtggctatc ggggcatcgt
     1561 gcgccttttt gactccaata ggtcatcaaa ataatttgtt ggtcatgggg cccggaggat
     1621 atcgatttgg ggactattgg cggatgggat tgcctttaga gttgttaatt cttgtggtct
     1681 cccttcctct cttagtcttc atgtggccct taacgggtca agggtaacgg ctttctgaat
     1741 aagaattaag aggaaaaaag tgtcgttctc attccgaacc cattcttccc gaaagtcctc
     1801 ccgtctcttt tttaggcggg tggtctacat gcccaccgac cttgatccgt ttcgcacgct
     1861 cttgagcaga attatattgg tggtcatgct gttttgtttt ttgacgggga tcctgtggtt
     1921 ggatcgaaac gggctcaagg atcagataga cggagagatc tcattttcag atgtgattta
     1981 tttcacgatg gtcacggtga ccacagtcgg ctatggggac attgtgccgg tctcgaccag
     2041 agcccgcctg attgatgcat tcgttgtgac gccggtccgc atatttctat gggtcatttt
     2101 tttagggacc gcctatcaat tggcgtttag acaatttacc gaggtatttc gcatggcaaa
     2161 actccagagg tctcttgatc aacatgtcgt catctgcgga ttcgggcata ccggatactc
     2221 gaccgtcaaa gaactgctcg ccaagggcac aaaccctgac catattctgg tcatcgatcc
     2281 cggtgaggaa cgagtacgca tcgcgggaga attgggagtg gtggcactcc gaggcgatgc
     2341 gacgcaggaa ggactgttga agtatggagc ctttctcaat gaagcgaagg ccgtcattat
     2401 ttcggcaggt cgggacgata caaatgccct tattctgtta accgtccgtc atttgaactc
     2461 acgttgccgg gtcatcgtca gcgccaagga agaagaaaac gtcaaattgt ttcgccaagg
     2521 cggggcgcaa accattattt ccccggctac gtatggcgga tatatgttgg cggccgcagt
     2581 ggaccaacaa tatttagcgg attacttaga agatttttta acggccggcg gaaaggtcaa
     2641 tattgaggaa cgaacagtcg acaaacagga tatcggaaag tcggcaatgg atttacagcc
     2701 ggatgtgctg ctcagggtgt atcggcaagg atcgattctt tccccctggg aatttcaaaa
     2761 aaatcatagg ttggaacagg gcgatgtcat gttattgttt aaaccggttt cggaggatat
     2821 gccttcctct tgaagaaaat ccctcccatt cctaaaagag gagtggcctt gcgttggcca
     2881 tgattaataa aattccgatg accccgatta aaatggccac gccccgtctc aaatggtgag
     2941 caggaatcga ttgagtgact tgtgagccaa acagtaaccc gagcagactg ccgagtaaca
     3001 gtaatcctgt tagcaccata tccaggcgtg ccccctcgaa atggccgatt acccctccga
     3061 tggaaataag ggcaataatc aaaagtgacg tgccaatcgc taagcgaatg ggaaacccca
     3121 tgagaaacat caaggcggga acaatgagaa accctcctcc aactccaaaa aatccggtca
     3181 ataggccaac tccccatccg aagatgaggg ctttccacgc acagcgccag gagaattctt
     3241 tggcgcatcc gctcgggacg tcgttgtgtt ctccttcata atttttccaa cacgtccaca
     3301 tgctaatgaa caggagcaga ttgccaaatg caaaaaggac caaggagtcc ggaacgagtt
     3361 ggtggccatg agccccagcc caagcaccga tcattccggt caggctgaat aaaatagcag
     3421 agacaaaatg aacacgttgc tgtcggctct catgccatgc cccaaagagg gccgaatagc
     3481 caaccacgat cagtgacatg gtcgtggcat tttgaaccgg tacgccaacg acatacacca
     3541 ataagggaat ggccactaat gatccgcccc ctcctgtagc acctaacagc actccgacga
     3601 tcccaccaga cagaaacgcc aatagataat ggaagtccat gagaaattag gttgaaagcg
     3661 gaacgggtga aagaataaga ccagaggcgt cagaggcgtg cggcatgctt taataagcaa
     3721 aatggaatat gtacgcaagc ataaccggca ataaaatgtt acaggaaaaa cgataggaaa
     3781 aaatgagagg agaatgtgat ttctccccac ttggaaacca tgaaaggata gtgtcgatca
     3841 tctatttttc actgagtaac gaatccatga ttctggtcaa cctgaacttt ttaagttttc
     3901 agatgatatg cataatctcc atctccagaa atcgccctac cccatgcgtt aaggcgaaga
     3961 ttcaaccggt attccctcag gccgaaacac tcatcgaggc cacgctcata aatactcaga
     4021 catcatatac ttcacgaaat ctcccgagta taaatgaaaa atcaatgaaa gaggagtaca
     4081 cgttcattgt atggggagaa ttcaaatgag acaagagtcc tcagatca
//
LOCUS       DATIAL010000542         8754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1063267, whole genome shotgun sequence.
ACCESSION   DATIAL010000542 DATIAL010000000
VERSION     DATIAL010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063267"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VLA60_16560"
     CDS             <1..439
                     /locus_tag="VLA60_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11025.1"
                     /translation="ISNRERLALQAKADRWPPNDILVDIRPDNPGGEYPIQGAFRLRS
                     FHGILNFLGRSIASEPEYHVDPDPGTGIVAENPVRVLDIIESTIERPNTKLSVTHEGH
                     YYSIADEEKRSWNQEAFRLLYQLFQMTVTDAPRGNVPSITIAK"
     gene            1106..2419
                     /locus_tag="VLA60_16565"
     CDS             1106..2419
                     /locus_tag="VLA60_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF11026.1"
                     /translation="MTFLIRFIHALVITVVMVGVQCENAWAQYDSITASKQAPGAAPT
                     TVILSLNDSLSLFLEKNLDLLMTKYGIDNAKGIAITAKLFPNPTFLLYGGAAFTGKQT
                     FAGTRYITPQLEQMFLLAGKRGYRMESAGYGIQASEATFTDAIRQLTLTLKDTYYQVQ
                     LASRRLDLAKDNQERFHRILTIGELRFKKGFIAEVDLIRLRLQAVDFGAQVIKFTQEV
                     QTALADLRLLLAFPPTTDLVLTSDLIYKRVTPDMERLRTEALNKRPDLQARRFVLSQQ
                     QANLKLAKSLRIPDPIVGGAFTMQGPQGGSNQQLYSLNLEVPLPVFDRNQGGIAQAEI
                     AIQVAQVDLHKTTLEVQNEIEVNYRNLTQSQHLVEAYQAGVLDDAKTTFSILEKAYQK
                     GGVTLIDLLDAARTSQTILQNYLEALFEYQRNLFLLERAAGQDIL"
     gene            2416..3633
                     /locus_tag="VLA60_16570"
     CDS             2416..3633
                     /locus_tag="VLA60_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HSF11027.1"
                     /translation="MTSGLRLCRCVLFMVILIGVACSQESPSETSATTEPVQTTRDKA
                     HHSLSTPETQIVTAIVTVGPSHPVLSLAGKVSYGEDRYSKVSSALVGRVQKIHGQLGD
                     FVKAGDLLVTIESPEIASAYSEFIKEHSDLSYAQRSYSLAKDLYEIKALPQKDLKQAE
                     NDYVKAKAEFRRAREKLLALQVSKEELDKPIADQTITSTYQIRSPLSGTIVDRAVTPG
                     QSVSGDPNQVLFTLADLKTVQVIADVYERDLGLVKIKQQATVTVEAYPETAFPATIAA
                     IGDVVDQTTRTIKLRAVVDNSDRKLKPGMFARLNVKLGESLHYPLIPQEAVLEIDGKM
                     YVYVADSDKHYTKRPIKTGLPSSGQMPVLHGLEAGETIVVKGAVLLKGQDMNVEDEGL
                     SSEHLAPTSPPKS"
     gene            3630..6764
                     /locus_tag="VLA60_16575"
     CDS             3630..6764
                     /locus_tag="VLA60_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HSF11028.1"
                     /translation="MIGRLVELSLVQRALVCTFGIFLLFGGLYAFHILDVIAYPDPSP
                     PFVEVISQKSGWSAEEMERIITIPIETALQGIPGLTNVRSLSLFGLSELKVYFEFGTN
                     WYAVRQEVLNRLHTVDLPDGVKPELSPWWAIAEIYRYELVGDGYSLTDLKTIQDWQVR
                     REFKQVPGIIDVTAFGGTTKEYHVDLDPGALLTYGVTLAQIKAALANSNANVGGSYLA
                     LGPQSYNVRGVGFIDSLEDIAKVVVAVKDGTPIFIENLGKVSIGNAIRLGQVGINEVE
                     DTLEGVILLQRDTQALPTLARVKQKVQDLNQKKLPAGVHIKTIYDRTTMINTVIETVV
                     HILINGMVLVLIVLLFFLGHFRTALIVACTIPLALLFTFSAMVIMGQSANLISLGAID
                     FGIIVDAPLVMVESIFFYLCHHAKPGVTPPQLIARAARHVGRPILFSTVIIVVAFIPL
                     FTMTGVPGKIFAPMSITYGLALAGSLLLACTLAPAMCSFLLNGPMREQEPKLVSTLRQ
                     TYLAALRWGLHHQKKVLGVVGMLLAVTVLALNFMGGEFMPALEEGNLWVRIRMPIDIR
                     FDDASALASRMRKMFMESPEVATAVSQLGRPDDGTDPESFFNVEYYVNLKPRAEWRPG
                     LTKEGLIEEIEGRLETIPGLKVNFSQLIQDSVEEAMSGVKSENSIKLYGHNLTELQSL
                     AEKVEGELKNIRGVKELSINRTMGQPNLLIQVDRQACARYGIQVGDVNAMVQAAIGGE
                     AVTEVIEGDRRFELVVRFLPQYRQDETTIGQIQVSSPEGVGIPLKQLATIVRQTGAFM
                     IYRENHERYIPIMFSIRDRDLVSTIREAQRHLEDRITLPEGYHLEWAGQYDQLVKEQQ
                     RLMIVVPLTMILILFLLYLTFGSFRYAFIVLATVPFAMIGGVLSLVLTHTPFSISAAV
                     GFISTLGIAILGGVLIVSSIRELEQNGIPLKEAILSGAELQMRPVLMATLGAALGLVP
                     AALASGIGSEAQKPLARVVVGGMLTATFLILFVVPILYQLTSQYSKRRSRPQPDSEEQ
                     EHINQDATHSSGRP"
     gene            complement(7077..7547)
                     /locus_tag="VLA60_16580"
     CDS             complement(7077..7547)
                     /locus_tag="VLA60_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11029.1"
                     /translation="MRKRIIDHVQQGTEPPGEWLNVEELAEVEITSEDAAFPIEGALV
                     AGGTSGWRAAGPGKQTIRLLFATPQPLRRIWLNFLETRIGRTQEYVLRWSPDGGQSFR
                     EIVRQQWNFSPDGATSQTEDHHVELPAVTVLELSIIPDTSGGPALASLAQLRLG"
     gene            complement(7569..8363)
                     /locus_tag="VLA60_16585"
     CDS             complement(7569..8363)
                     /locus_tag="VLA60_16585"
                     /inference="COORDINATES: protein motif:HMM:NF005095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HSF11030.1"
                     /translation="MILIRNSPDELSDKRALVTGGTKGIGEAIVKRLRLSGATVMTTA
                     RSQADDLQASELFIQSDISTVDGVNKVVKEVLSRLGGLDILINNVGGSSASSGGALAI
                     HDDDWQQTFNANLFSAVRLDRAFLPKMLEQRSGVIIHISSIQRTLPLYDATLAYAAAK
                     AALTNYSKGLSNEVAPKGLRVISVAPGFIETAAATGLIERLAKNTGTDFDAARQELMN
                     SLGGIPIGRTGRPEEVAELVAFLVSDRAAYINGTEIVIDGGTIPTV"
     gene            complement(8565..>8754)
                     /locus_tag="VLA60_16590"
     CDS             complement(8565..>8754)
                     /locus_tag="VLA60_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11031.1"
                     /translation="TWSAIFPLVLVIPLVVAMGLRQLGVPHNHYLDLLLSTGSVVWVM
                     VYLVMPHYTKLIQRWLFT"
BASE COUNT         2116 a         2419 c         2143 g         2076 t
ORIGIN      
        1 catctccaat agagagcggc tggctctgca ggccaaggcg gatcgttggc cgccgaatga
       61 tatcctggtc gatatccgac ccgacaaccc gggaggcgaa tacccgatac aaggggcctt
      121 tcggctccgg agttttcacg gcattctcaa ttttctgggc cgatccatcg cgagtgagcc
      181 ggagtatcat gtggatccgg atccaggaac gggaatcgtc gcagaaaatc cggtccgggt
      241 cttggacatt atcgaatcca ctattgaacg ccctaatacc aaactttccg tcacccatga
      301 gggacattat tattccatcg ccgatgagga gaagcgatcc tggaatcagg aggcattccg
      361 gttgctgtat caattgtttc aaatgaccgt caccgatgcc ccccgcggaa atgtccccag
      421 tatcaccatt gctaaatgaa aattatgcgc ataaacaatc caatccgctt acataagtaa
      481 aatctcttta cctgctcaaa gaacagtggg gtgagactct tgaggttgaa cttagtcttt
      541 tccggctcca gggtctccca tcccagacga tatgaaggcg gattacccat acgcaccttt
      601 cccctcgtcg cccccaacag agtgatgaca ggctgtcctt tcccggtaca gacattccct
      661 ggtgcacacc ttcatgtgcc tacctaaaac ccttcatggc aatgttagag ggtataaacc
      721 ggttgtttcc tataaaggtt ttcttccctc ctcatctttg caaatgttac ttacccatgg
      781 aatgcctctt gcaatgtgta attcacgagg tccatgtgaa gatcttcttt accgaagcta
      841 tttccaaaaa tagtaggggg gagggaaacg tgtgcggctt actttcggca gattgtccgg
      901 cattagtaaa attatcatct tgctctcagg ggttctaggg cgataaggta cactcccttt
      961 taacctgggg ttttttcttt tacctccggt tctcaggtct cgatatacgg aaaaggaagg
     1021 ttgccattgg gtagcttttg ttctccactc tcctcccctt cgtggtgatc caggcgtccc
     1081 cggattatca attcctgcca ccaacatgac attcctcata cgtttcatcc acgccctggt
     1141 cattacggta gtcatggttg gggttcaatg cgaaaatgca tgggcgcaat acgattcaat
     1201 cacagcttca aagcaggcac caggcgcagc tcccaccacg gtgatcttga gcttgaacga
     1261 ttccttatcc ttgttcctgg aaaaaaatct tgatctcttg atgacgaaat acggcatcga
     1321 caatgccaag gggatcgcca tcacggccaa actttttccc aacccgactt ttttacttta
     1381 tggaggagcc gcatttaccg ggaagcaaac ctttgcaggc actcgctata tcactcccca
     1441 gcttgaacag atgtttttac ttgccgggaa acggggctat cggatggaaa gcgcgggcta
     1501 tggcatccag gcttccgagg cgacattcac ggatgccatt cgtcaactga cgctcaccct
     1561 gaaagacacg tattaccagg tccaattggc atcccggcgt cttgatctcg caaaagataa
     1621 tcaggagcgg tttcatcgga tcttaacaat tggggaatta cgctttaaaa agggcttcat
     1681 tgccgaagta gacctcattc gtcttcgcct ccaggccgta gacttcggag cacaggtcat
     1741 caagtttacg caggaagtcc aaaccgcact cgctgacctc cgactactcc tggccttccc
     1801 gcctacaacg gatctggtgt taacctccga cctcatttat aaacgggtaa cacctgatat
     1861 ggaaagactt cggacggagg cgttgaacaa acgtccggac cttcaagcca gacggttcgt
     1921 cctttcccag caacaagcca atttaaaatt agccaaatca ctcaggattc ccgatcccat
     1981 tgtcggaggc gcattcacca tgcaggggcc gcaaggcggg agcaaccaac aattatatag
     2041 cctcaacttg gaagtgccgc tcccggtctt tgaccgaaat caggggggca ttgctcaggc
     2101 cgaaatcgcc attcaagtcg cgcaagtcga tcttcacaag acgacgttgg aagtccaaaa
     2161 tgagattgaa gtgaattacc ggaatttaac ccaatcccaa cacctggtgg aggcctatca
     2221 ggcgggagtg ctggacgatg ccaaaaccac attttccatt ctcgaaaaag cctatcaaaa
     2281 gggtggagtt acactcatag atttgctcga tgccgcccgc acctcacaga ccatcctgca
     2341 aaactatttg gaagccctct ttgagtacca acgtaaccta ttccttttag aacgagccgc
     2401 cggccaggat attttatgac atccggactt cgtttatgcc ggtgcgttct tttcatggtg
     2461 attctcatcg gagttgcctg cagccaagaa agcccctcag aaacttccgc gacaacggaa
     2521 ccggtccaga cgacacgcga caaggcccac cactctctct caacaccgga aacacaaatc
     2581 gtcacagcca tagtgactgt tgggccctcc caccccgtct tgtccttagc agggaaagtg
     2641 tcctatgggg aggatcgata ttcaaaagtc tcttccgccc tggtcggtcg ggtacaaaaa
     2701 atacatggac aattgggaga tttcgtgaaa gcgggagatc ttctggtcac cattgaaagc
     2761 ccggagattg cttcggcgta ttcggaattc atcaaagaac attccgactt atcctacgcg
     2821 caacggtctt acagtctggc caaagacctg tatgagatca aagcgctccc tcaaaaagat
     2881 ttgaagcagg ctgaaaacga ttacgtgaaa gccaaagcgg aattccgaag agcccgggaa
     2941 aagctcctgg cccttcaagt ttccaaggag gaactcgata aacccatagc cgaccaaacg
     3001 attacgtcaa cctatcaaat ccgaagcccc ctttccggca ccatcgtgga tcgggccgtg
     3061 accccgggac aatcggtcag cggggatccc aatcaagtgc tgtttacgtt agcggattta
     3121 aagaccgttc aagtcattgc ggacgtctac gaacgagacc tgggacttgt aaaaatcaaa
     3181 caacaagcga cggtaaccgt cgaagcgtat ccggagacgg catttccagc caccatcgca
     3241 gccatcgggg atgtcgtgga tcagacaacc cgaacgatta aactccgggc ggtcgtggac
     3301 aatagcgatc gcaaactcaa gccggggatg tttgctcgtt taaatgtcaa actgggcgaa
     3361 agccttcact atcctcttat tcctcaagaa gccgtgctgg aaattgacgg caagatgtat
     3421 gtctatgtcg cggatagcga caagcattac acgaaacgtc cgatcaaaac cggactcccc
     3481 tcctccggcc aaatgcccgt cctccacggt ctcgaggcgg gcgaaacgat cgtcgtaaaa
     3541 ggggccgtac tcctcaaagg tcaggacatg aatgtcgagg atgagggatt gtcctcggaa
     3601 caccttgccc ccacttctcc cccgaaatca tgatcggcag acttgtcgag ctgtcactcg
     3661 tccaacgtgc tctggtctgc acattcggga tcttcctcct ctttggaggg ctctatgcct
     3721 ttcatatcct tgacgtgatc gcctacccgg atccctcgcc gccctttgtc gaagtcattt
     3781 ctcaaaaatc gggatggtcc gccgaggaaa tggagcgaat catcaccatt cccattgaaa
     3841 ccgctctcca gggtattccc ggccttacca atgtgcgttc cttgtctctt tttggattaa
     3901 gtgaactcaa ggtctatttt gaatttggca ccaattggta tgccgtccgc caagaagtgc
     3961 tgaatcggct ccacaccgtt gatctgcccg atggcgtcaa accggaactc tctccctggt
     4021 gggccatcgc agaaatttat cgatatgaat tagtgggaga cggctattct ctcaccgacc
     4081 tcaaaaccat ccaggactgg caggtccggc gtgaattcaa acaggtacca ggcatcattg
     4141 atgtgacggc gtttggaggc accacaaaag aatatcacgt ggacctggat cccggtgccc
     4201 tcctcaccta tggagtcact cttgctcaga tcaaagcagc tctcgccaat agcaatgcca
     4261 atgtcggtgg tagttatttg gccttaggac cccaaagtta caatgtcagg ggagtgggat
     4321 ttatcgacag cttggaggac atagccaagg tcgtcgtagc ggtcaaggac ggcactccca
     4381 tctttattga aaatctcgga aaagtgtcca ttggcaatgc gattcgtttg ggacaagtcg
     4441 gcattaacga ggtggaagac actctggaag gggtgattct tctgcaacgg gacacccagg
     4501 ctctgcccac cctggcacgc gtcaaacaaa aggtacagga cttaaaccaa aaaaaacttc
     4561 ccgcaggggt ccacatcaaa accatttatg atcggaccac catgatcaac accgtcattg
     4621 agacggtcgt gcacatatta attaacggca tggtattggt gctcatcgtt ctcctgtttt
     4681 tcttgggaca tttccggacc gccctgatcg tcgcctgtac gattcccctg gctctgctgt
     4741 tcaccttttc cgccatggtc atcatgggac aatccgcgaa tctcatttct ctgggtgcca
     4801 ttgatttcgg gattatcgtc gatgcccccc tggtcatggt cgaaagcatt ttcttttacc
     4861 tgtgccacca tgccaaaccc ggggtcaccc ctcctcagct cattgcgagg gccgcccggc
     4921 atgtgggacg accaatctta ttttcgacag tgattatcgt ggtggcgttc attccccttt
     4981 tcaccatgac gggggtcccg ggaaaaattt ttgcgcccat gtccattacc tatggtctgg
     5041 ccctggctgg ttccctgctg ctggcctgca ccctcgcccc ggccatgtgt tccttcctgc
     5101 tcaatggacc catgcgcgaa caggaaccca aactggtttc cacgctcagg caaacctatc
     5161 tggccgctct tcgctgggga cttcatcacc agaaaaaggt gctaggcgtg gtagggatgc
     5221 tattggccgt gacggtttta gcactcaact tcatgggtgg agagttcatg ccggcgttag
     5281 aggaagggaa cctctgggtg cggattcgca tgccgatcga tatccggttt gacgatgcct
     5341 cggccttggc cagccgcatg cggaaaatgt ttatggaatc ccccgaagtg gccactgcag
     5401 tctcacagct cggacgcccg gatgatggca cagaccccga aagttttttt aatgtggaat
     5461 attacgtcaa tctcaaaccc cgggctgaat ggcgcccagg cctgactaaa gaaggtctga
     5521 tcgaagaaat tgagggacgc cttgaaacga tccccggcct caaggtcaac ttctcccaac
     5581 tcatccagga cagtgtggaa gaagccatgt cgggcgtgaa gagcgaaaat tccattaagc
     5641 tctacggaca caacctcaca gaacttcaat cccttgcgga aaaagtagaa ggtgaactca
     5701 aaaacatccg cggagtgaaa gaactcagca tcaaccggac catgggacaa cccaacctcc
     5761 tgattcaagt tgaccggcaa gcctgcgccc gttacggcat acaagtcggc gatgtcaatg
     5821 cgatggttca ggccgccatc ggtggggaag ccgtcacgga agtgatcgaa ggagaccggc
     5881 gatttgaatt ggtggttcgg tttcttcccc aatatcgaca agacgaaacc acgattgggc
     5941 agatccaggt cagttctccc gaaggagtcg gcattcccct aaaacaatta gccacgattg
     6001 ttcgccaaac cggggccttc atgatctatc gagaaaatca cgagcgatat attcctatta
     6061 tgttcagcat ccgtgaccgg gatctggtga gtacgattcg agaagcacaa cgacacctcg
     6121 aagaccggat cacactgccg gaagggtatc atctcgaatg ggcgggacaa tatgaccaat
     6181 tggtcaaaga acaacaacgc ctgatgattg tcgtacccct gactatgatc ctgattctct
     6241 ttttgctata cctcacattt ggttcattcc gatacgcctt tattgtgctc gcgacggttc
     6301 cctttgccat gatcggaggg gtactctccc tggtcctgac tcataccccc tttagcattt
     6361 ccgccgccgt aggattcatt tccaccttgg ggatcgccat ccttggcggc gtcttaatcg
     6421 tctccagtat ccgggagttg gaacaaaatg gcatccccct caaggaggct atcctttcgg
     6481 gagcggagct gcagatgcga ccggtgctaa tggccactct gggagccgcg ctgggcttag
     6541 ttcctgccgc gctcgcaagt ggcatcggtt ccgaagctca aaagccattg gcccgcgtgg
     6601 ttgtaggcgg catgttgacc gccaccttcc tcattttatt cgttgttccc atcctgtacc
     6661 aattgaccag tcagtactct aagagacggt cccgacctca acccgattcc gaagaacagg
     6721 aacacatcaa ccaggacgct actcatagca gcgggcgtcc ttaaccattc gaacgggttg
     6781 ccttccaact cgtacccgat tttccaaatt ttctaaaaaa cttggggttt catttttcta
     6841 aagtgggact ctatgagaaa aatttctggg atccgtttcc attgtgctta atgacgatgg
     6901 ccatctcatg cagcattaaa ggaacggtaa tttcaaacag tcctcctacg accaaatggg
     6961 tgcatggtga aactcaatca aagtttatac caggctggtc gattcctcag gtggaatcgg
     7021 ccaaattcag ttgcgatgat ttgtcaccag gctttgacaa ccggtttcgt ctagaattaa
     7081 cccagccgca actgcgccag cgacgcgagc gcggggcccc cactggtgtc cggaatgatg
     7141 cttaactcga gcaccgtgac cgccgggagc tcgacgtgat gatcttccgt ctggcttgtt
     7201 gcgccatcgg gactgaagtt ccattgctgc cgcacgattt cccggaacga ctgcccgcca
     7261 tccggcgacc aacgcaacac atactcttgc gtgcgcccga tgcgggtttc caaaaagttc
     7321 agccagatcc tccggagcgg ttgcggagtg gcaaagagaa gccggatcgt ctgtttgccc
     7381 ggccctgcgg cacgccaccc ggaagtccca ccggccacca acgccccctc aattggaaaa
     7441 gcggcgtcct ccgaggtgat ttccacctca gcaagctcct ccacattcag ccactcccct
     7501 ggcggctcgg ttccttgctg aacgtgatcg ataattcgtt tgcgcattca atatgccctc
     7561 ctgatcattt agactgtcgg aatggttccg ccgtcgataa cgatttcagt gccgttgata
     7621 taggccgcgc gatcggacac cagaaaagca acaagctcgg caacttcttc tgggcgaccg
     7681 gtgcggccta tgggaatacc tccaagggaa ttcatcagtt cttgccgggc ggcatcaaag
     7741 tctgtccccg tattcttagc cagcctctcg atgagtccag tagcagccgc cgtctcgatg
     7801 aacccgggtg ccactgaaat aactcgcaat cctttgggcg caacttcgtt tgaaagtcct
     7861 ttgctgtagt tggtcagagc cgctttcgcg gcggcatatg ccaaggtggc atcataaagt
     7921 ggaagggtgc gctggataga cgaaatgtgg atgataacgc ccgatctctg ttccagcatt
     7981 ttgggtagaa atgctcgatc aaggcgaaca gcggagaaca agtttgcatt gaatgtttgc
     8041 tgccaatcat catcgtgaat agcaagtgcg ccaccgctgg atgcagatga gcccccgaca
     8101 ttattaatca ggatgtccag tcccccgagc cgggagagca cttctttcac cactttattt
     8161 acgccatcga cggtactgat gtcggattgg ataaacaatt ccgatgcctg aaggtcatcg
     8221 gcctgtgagc gggcggtcgt catcacggtt gcaccagaca gcctcagtcg tttgacgatg
     8281 gcctcgccga tgccttttgt gccacccgtc accagcgctc tcttgtcgga taattcgtct
     8341 gggctatttc ggatgagtat cataaaatgt ggtcctgttg tttgatgcgc atagtgacgc
     8401 ctaactatag gttatctgat tccagattag tctaccggtc cgatccacca gcagcaaatg
     8461 tctctgggca tctgaagtca cccatcatgt ccttcaccac accaagttga gcgcagactc
     8521 gtgtctacgg ctgaagcgat tttcgaggcg agtgcttctc ggaatcaggt aaacaaccag
     8581 cgttggatca gtttggtgta gtgcggcata acgagataga ccatgaccca taccaccgaa
     8641 ccggtgctaa gcagcaagtc cagataatga ttgtgaggta ctcccagttg ccgaagcccc
     8701 attgcgacaa ccaggggaat gacgagcacc aacggaaaga tggcggacca tgtt
//
LOCUS       DATIAL010000543         4074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1279301, whole genome shotgun sequence.
ACCESSION   DATIAL010000543 DATIAL010000000
VERSION     DATIAL010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279301"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VLA60_16595"
     CDS             <1..632
                     /locus_tag="VLA60_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF11032.1"
                     /translation="ILKTIHAKDVDLVVLGSRGYSKAKGILLGSVSQWVLQEAPCSVL
                     IARPMAREKKAVRGMKLLLATDGSADSRAAVEFIKNLTLPPSSQITILHVVKKHVFET
                     EQTLIADSKKEDEFAKLAEELLAIRGREGAKLLEQTRKVLSAPHLQIQERLVFGNPGD
                     EILKAVRYVRADLVVLGSRGSTGVKRVFLGSVSNKVVYGAGCSVAVIRH"
     gene            836..3544
                     /locus_tag="VLA60_16600"
     CDS             836..3544
                     /locus_tag="VLA60_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HSF11033.1"
                     /translation="MSIRNFKYLFKPRAITVIGASNRPHRVGSVVMQNLLKGGFEGPI
                     LPVNPKYQAVCGVLAYPDVKALPVNPDLAILCTPPDTIPRLLQELGERGVRAAVILTA
                     GMRPEPSGKEGATGWTDMLDTARKFGMRLLGPNTVGMLIPRLGLNASFAHVEALPGSL
                     AFLSQSGALCTAVLDWARSKGIGFSHFLSLGDSADIDMGDVLDYLGSDPDTRAILLYI
                     ESITQARKFMSAARAAARNKPVLAIKAGRHPEGATAASSHTGALIGCDEVYDAAFCRA
                     GILRVFEIDELFDAVETLERARPLQGERLAIVTNGGGPGVLAADAFAEGGGALAGLSE
                     ATIQELTALLPASWSHGNPVDIVGDARGDRYAQALTSILNAPDVDAVIVTHAPTAVVT
                     SEEAALAVVNAVRASSSKKNVLTSWLGGQTTLQARRMFAEANIPTYGTPEKAVRAFLH
                     LIRYSQRQRMLMEIPPSIPREFQPATSSARYVIEQELAQGRSLLNEPEAKAILAAYGI
                     PTVETHIASTPEQAIRFAEQVGFPVALKILSPDITHKTDVGGVVLDVENSKAVATAFQ
                     GMKDRLQQLHPGAEMRGVTVQPMARRPGAYELLVGVTVDAAFGPVIVFGQGGIAVELL
                     GDHAMALPPLNMKLAKDLIKATRIASLLKGFRTHPAVDLEALCLTLMQISHLVQDFPE
                     IVELDINPLLADQNGVLALDARMKIAPSPVSGPHHLAIRPYPEELEEWMTLRSGKRIL
                     CRPIRPEDEPAHNEFFSRLSQEDIRFRFFGLIRELPHSQMARFTQIDYDREMAFIAVG
                     KDEANRSETLGVVRTANDANNHTAEFAIIVRSDMKGQGLGRLLLEKMIRYCRQRGMAQ
                     IMGQVLTDNTGMLELARRTGFTKKLTAEGDAIEITLSLTSRWASEK"
     gene            3534..>4074
                     /locus_tag="VLA60_16605"
     CDS             3534..>4074
                     /locus_tag="VLA60_16605"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11034.1"
                     /translation="MKNDRPLFYEVAAERTLDRAMGHGFDGILADQEQYMDDFWKRSD
                     VQIRDINPKWAKGSTIEIQQAIRFNLFHILQAAGRADTLGVPAKGLTAQAYEGQYFWD
                     TEIYLFPFLIYTSPRLAKNLLMFRYRMLDHARERARELNQKGAMFPWRTINGKEASAY
                     YAAGTAQYHINADIMYALRK"
BASE COUNT          896 a         1029 c         1191 g          958 t
ORIGIN      
        1 aaattttaaa aaccattcac gcgaaagacg tcgatctggt ggtcctcgga tcacgtggct
       61 attccaaagc gaaggggatt ttactcggga gtgtgagtca atgggtgtta caagaagcgc
      121 catgctcggt gttgatcgct cgccccatgg cacgggagaa aaaagctgtt cgaggcatga
      181 agcttctcct tgctactgat ggatctgccg attcacgagc ggccgttgaa tttattaaaa
      241 acctgacatt acccccttcc tcacagatca ccattctcca tgtcgtcaaa aaacatgtgt
      301 tcgaaacgga gcagacgttg attgccgaca gcaagaagga ggatgaattt gccaaattgg
      361 ccgaagaact cctggcgatc cgaggccggg aaggagcgaa actccttgaa cagactcgaa
      421 aggtcctgag tgctcctcac ctccaaattc aggagcgatt ggtgtttggc aatccaggag
      481 atgaaatttt gaaagctgtt cgatatgtgc gagctgatct tgttgtcttg gggtcgcgtg
      541 gctcgaccgg ggtcaagcgg gtgtttctgg gcagtgtatc gaacaaggtg gtctacggtg
      601 ccggatgttc ggtggcggtc atacggcatt aagcgaggga gtggtgctcg gaaatcttgg
      661 agaagaaagc tgtcatgttg catgaacatg gttctagcgt ggcttgcccc gctcccacgg
      721 aagtggacgg cagaatggac tcgctgctcc ttccttaccc gatcccgtac ccaccgtggc
      781 ggtcggtatt gcagaagtgt ttgccattat tcatctgtga ataaaggtac gtgtcatgag
      841 cattcgaaat ttcaagtacc tgttcaagcc acgcgctatt acggtgattg gtgcttcgaa
      901 tcggcctcat cgagtgggaa gtgtggtgat gcagaatctt ctgaaagggg gattcgaagg
      961 ccctattcta cccgtgaatc caaagtatca ggcggtgtgc ggggtgctgg cgtaccccga
     1021 tgtgaaggcc ctgccggtca atcccgatct ggccatactt tgtactcctc ccgataccat
     1081 tccccggttg cttcaagaat tgggagaacg gggagtgcgg gctgcggtca ttctgacagc
     1141 cggcatgagg ccggaaccat cagggaagga gggtgcgacc ggttggacgg acatgctgga
     1201 taccgcaagg aaattcggca tgcgtcttct gggtccgaat acggtcggca tgcttattcc
     1261 ccggctggga ctcaatgcca gttttgccca tgtggaagcg ctcccgggct cgctggcgtt
     1321 tctttctcag tccggcgccc tttgtacggc tgtcttagat tgggcccggt ccaagggaat
     1381 cggcttttcc cattttttat ctctcgggga tagtgcagac atcgatatgg gcgatgttct
     1441 ggattatctg ggcagcgacc ccgatacccg tgccattctt ttgtacatcg agtccatcac
     1501 tcaggccaga aagtttatgt cagccgccag agccgccgct cggaataaac cagtcctggc
     1561 gatcaaagcg ggacgacatc cggaaggggc gactgccgcc agttcccata ccggggcact
     1621 catcggttgt gatgaggtgt acgatgctgc attctgccgg gctggaatcc tgcgggtgtt
     1681 tgaaattgat gaattgttcg atgccgtcga aaccctggag cgagcccgtc ccttacaggg
     1741 agaacgatta gccattgtga cgaacggagg agggccggga gttctggccg ccgatgcctt
     1801 tgccgaaggc gggggtgccc tcgccggctt gtcagaggcc accatccaag aattgaccgc
     1861 cctgttgccg gcaagctggt ctcacggaaa tcctgttgat atcgtcgggg atgcgagagg
     1921 ggaccgctat gcgcaggcat tgaccagtat cttgaacgcc ccggatgtgg atgcggtcat
     1981 cgtgacccat gctcccacag ccgtcgtcac cagcgaggag gccgcgctgg ctgtggtgaa
     2041 tgcggttcgg gcatcgtcct ccaagaaaaa tgtgttgacc agttggctgg gtggccaaac
     2101 aacccttcag gcccggcgca tgttcgccga ggccaatatt cccacctatg gcacacctga
     2161 aaaggcggtg cgcgccttcc tgcacctgat tcgatactcc cagcggcagc gcatgctgat
     2221 ggaaattccc ccatccattc cacgggaatt tcaaccggcc acgtcttccg cacgatacgt
     2281 gattgaacag gagttagcgc aggggcgttc tctattgaat gagccggagg caaaggccat
     2341 tctggctgcc tatgggattc ccacggtgga gacccatatc gcgtcgaccc ccgagcaggc
     2401 cattcgcttc gcggagcagg tgggatttcc cgtggcgttg aaaattcttt ctcccgacat
     2461 tactcataaa acggatgtcg gaggtgtcgt gcttgatgtg gaaaactcca aggccgttgc
     2521 aacagcgttt cagggaatga aagaccggtt gcagcagctt catccgggag cggagatgcg
     2581 tggtgtgacg gtccaaccga tggcccgtcg gcctggtgcg tacgaattac tggtgggggt
     2641 gaccgtcgat gccgcgtttg gcccggtcat tgtgttcggt caggggggca ttgccgttga
     2701 gttgttgggg gaccatgcca tggctctccc tcccttgaac atgaaattag ccaaggatct
     2761 gattaaggcg acgagaattg cttccttatt gaaaggcttc cggactcatc ctgccgtcga
     2821 ccttgaagcg ctctgtctga ccttgatgca aatttctcat ctcgtgcagg attttcctga
     2881 aattgttgag ctggatatta atccccttct cgcagaccag aatggggtat tggcattgga
     2941 cgccagaatg aaaattgccc catctcccgt ttccggtccg caccatttgg ccattcgtcc
     3001 ctatccggaa gaacttgagg aatggatgac gctgcgatcg gggaaacgga ttctgtgtcg
     3061 gcccattcgt ccggaagatg aaccggctca taacgaattt ttttcacggt tgtcccagga
     3121 ggatattcgg ttccggtttt tcggactcat tcgggaatta cctcattcac aaatggctcg
     3181 ctttacccag attgattacg accgggaaat ggcgttcatt gcggtaggca aggatgaggc
     3241 gaaccggtct gaaacccttg gggtggttcg cacggctaat gatgccaaca atcacacggc
     3301 cgagtttgcc attatcgtcc ggtcggatat gaagggacag ggtttgggac gactgttgtt
     3361 ggagaaaatg atccggtatt gccggcagcg gggtatggcg cagatcatgg gacaggtctt
     3421 gacggacaat accgggatgt tggagttggc gcggcgaacc ggatttacca aaaaactgac
     3481 agcagagggg gatgcgatag agattacgct gagtctcacc tccagatggg cgagtgaaaa
     3541 atgatagacc ccttttttat gaagtggcag ctgaacggac cttggatcga gccatggggc
     3601 atggattcga tggcattttg gcggatcagg agcaatacat ggacgatttc tggaaaagaa
     3661 gcgatgtcca gattcgcgat ataaacccaa agtgggccaa aggctccacc attgaaatcc
     3721 agcaggccat tcgcttcaat ctgtttcata tcctgcaggc agcggggcgg gctgataccc
     3781 tcggtgtccc ggccaagggg ctcaccgccc aggcctacga aggccagtat ttttgggaca
     3841 ctgaaatcta tctttttccc ttcttgattt atacctcccc gcgccttgcc aagaacctcc
     3901 tcatgtttcg gtatcgcatg ttggaccatg ccagagagcg agccagagaa ttgaatcaga
     3961 agggagccat gtttccctgg aggaccatta acggtaaaga agcctcggcc tattatgcgg
     4021 cgggaacagc ccagtaccac atcaatgccg atattatgta tgccctgaga aagt
//
LOCUS       DATIAL010000544         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_849142, whole genome shotgun sequence.
ACCESSION   DATIAL010000544 DATIAL010000000
VERSION     DATIAL010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849142"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VLA60_16610"
     CDS             complement(<1..372)
                     /locus_tag="VLA60_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HSF11035.1"
                     /translation="MKEDSQVASQQNAFEKVVEFVDERVGLKNIQAKMLNEPIPGGSR
                     WAYAFGSVLLFIFILQVVTGILLMFYYVPSTDHAYASTQYIIHEVDYGWFLLSYHFWG
                     SSAMVVMVFAHMSQVFLWGAYK"
     gene            complement(401..1345)
                     /locus_tag="VLA60_16615"
     CDS             complement(401..1345)
                     /locus_tag="VLA60_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HSF11036.1"
                     /translation="MQPKLTAKALCANKEVGKISKVIVDPLSHEISHVIVHELNGHGA
                     ERQIPIGQVQEVVSEEEVVLRCSPEEFGQFPVLERDQYVTIKEVEIAHLEDHLHVEPG
                     EILVPLPRLEQGVPRRTFFTNMTHAIGTLIALPLVFPVLKYLMKPMFKPYDNAWFSVG
                     NVKKVNKENVGFQFKFTRGFKEAFMPEQQIEKNIWVVKATPAVQQAVYEGNDKKFYDD
                     KGDVIWVNKSNSPYIGYSGKCPHLGCGYKWRKTKNFPDGVFLCPCHLSIYDEAGKVID
                     GPAPRPLDVLPLQVDAGGEVKIIDVEYKAGVNNQIRLL"
     gene            complement(1372..2184)
                     /locus_tag="VLA60_16620"
     CDS             complement(1372..2184)
                     /locus_tag="VLA60_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF11037.1"
                     /translation="MANQSFAKSVMKKTALGVIIGLLLVVGAEYTHFPPVFQIMFFLY
                     AILGALVFILLDAPAMKRLEGVKAVGALVVFYLLLSGLYIAGASILPQFDPEDEKGKI
                     EKVLKLRRAQTEKGKADELIARAKELDERAKAISKQLSSLGADAKVEVAVATSGGGGK
                     AASGDLVALGKEQWELQECYNCHKLYGQGGKKRGPEMDNIGNLMTVEQLKEKIIDPKS
                     WKAEGFDKDYEKGKMPDKYKDLMFPQEIDALVAFLASLKDESVKTPKPIKMN"
     gene            complement(2209..>3402)
                     /locus_tag="VLA60_16625"
     CDS             complement(2209..>3402)
                     /locus_tag="VLA60_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11038.1"
                     /translation="LFVIDGPTSFMNTPAKAAGDLSLVEFIQTASLWDKMANFSWMPL
                     NLHRLVGNVTFGGFIAGLIAAYMFMGSKTDEERAYYDWMGFVGNMIGVGALLLLPFMG
                     YLLAYELCDYDASICPYMMADQLSMFFEMQGAMVGLIFLASNYYIWLSLKRIQGVEQV
                     RISGFVAVVVLLMPAIMGFTWKMFPPPEWQSLIVLVIIVVLPIILGKVPGLKNFTVSA
                     FTMIKIGFLMIVVADAIWMTPHGFVPTQGLATEELELPSWASELALMPAKNAAAFTLV
                     FLTVVNYLLYNRAIKRGTIVWGKIDFASQFVLIFLAFSAIWTMGLMGAVRSLTRKYYH
                     VYNLVPDFTPEAFTPTLAYSAWWITGVTIVFYAVVSFAILVTLRAGSPKPATSLASSV
                     PVEAK"
BASE COUNT          960 a          898 c          676 g          868 t
ORIGIN      
        1 tttataggca ccccataaaa atacttgaga catatgcgcg aagaccatca ctaccatggc
       61 agaggatccc caaaaatggt aactcagaag aaaccagcca tagtctactt cgtggatgat
      121 atattgtgta ctcgcataag cgtgatccgt actggggaca taatagaaca tcaacagtat
      181 ccccgtgacg acttgaagta taaaaataaa tagcaataca gagccaaaag cataggccca
      241 gcgagagcca ccaggaattg gctcattcag catttttgcc tgaatatttt tcagacctac
      301 ccgctcatca acaaattcaa caaccttttc gaatgcgttt tgttgacttg cgacttgact
      361 gtcttccttc atgcgccttt caccattcca tcctaggctt ttacagaagc cgaatttggt
      421 tattaacccc ggctttatac tccacatcaa tgatcttcac ttctccaccg gcatcaactt
      481 gcagaggaag gacatcgagc ggtctcggtg ccggcccgtc gataactttt ccagcttcat
      541 cataaatact cagatgacaa gggcacaaaa ataccccatc tggaaagttt ttcgtctttc
      601 tccatttata tccacaaccc agatgaggac atttgccaga gtagccgata tagggagaat
      661 ttgacttgtt gacccagatg acatctccct tatcatcata aaacttctta tcatttccct
      721 cgtatacggc ttgttggacg gccggagtcg ccttgaccac ccagatattt ttctcaatct
      781 gttgctctgg cataaaggct tctttaaatc cacgggtaaa tttaaattgg aagccaacat
      841 tctctttatt taccttcttt acattgccca cggaaaacca ggcattatcg tagggcttga
      901 acatgggctt catgagatac ttcaacacag gaaacactaa gggcaaggca attaaggtcc
      961 caatcgcatg tgtcatattg gtaaaaaatg tgcgcctggg aaccccttgc tctaaccgag
     1021 gaagaggaac caaaatctca ccgggttcca catgcaaatg atcctccaaa tgcgcaattt
     1081 caacttcctt aatcgtgacg tactggtctc gctccagtac cggaaattga ccgaattctt
     1141 caggtgaaca ccgaaggaca acttcctcct cgctcaccac ttcctgaact tggccaatag
     1201 gaatttggcg ttccgctccg tgaccattca attcatggac gataacatgg ctgatttcgt
     1261 gggagagtgg atcaacaata accttagaaa tcttcccgac ttccttgttc gcacacaatg
     1321 cttttgctgt caattttggt tgcatagcca gggaaaaacc tcagatgttg attaattcat
     1381 ttttattggc ttcggagttt ttactgactc gtcttttaac gaagcgagaa aggccacaag
     1441 cgcatcaatt tcttggggga acatgagatc tttatactta tcaggcattt ttcccttttc
     1501 gtaatcctta tcgaatcctt ccgctttcca gctctttgga tcaataatct tttctttcaa
     1561 ctgttcaaca gtcataagat taccgatatt atccatctca ggcccacgtt ttttcccacc
     1621 ttgtccatac agcttatggc agttataaca ttcttgtaat tcccattgtt ctttcccaag
     1681 ggctacaaga tcgccggaag ctgctttacc cccgcctcca ctggtcgcaa ccgccacctc
     1741 aactttagca tcagcaccta aagaactcag ttgcttcgaa atagccttag cccgttcatc
     1801 caattccttt gccctagcaa ttaattcatc ggccttccct ttctctgttt gggcccgacg
     1861 aagcttcaag actttttcaa ttttcccctt ctcatcctca gggtcaaact gtggcagaat
     1921 cgacgctccg gcaatataga gaccggagag caggaggtaa aataccacca acgcccccac
     1981 cgccttcacg ccttccaacc gtttcatcgc tggagcatcc aaaagaatga acaccaaagc
     2041 tcccagtatc gcgtaaagaa aaaacatgat ctgaaagact ggagggaaat gggtgtattc
     2101 agcgcccacc accaacagca gcccaatgat gacccccaag gcggtcttct tcatgacact
     2161 cttggcaaaa ctctggttag ccatttgttt cctctttttt caacctactc atttagcttc
     2221 aaccggcact gacgatgcca atgacgtagc cggcttcgga cttcccgctc tcagcgtgac
     2281 aaggatggca aaactcacga cagcgtaaaa cacaatagtc acccccgtta tccaccaggc
     2341 cgagtaggcc agagtcggcg taaacgcttc aggagtaaaa tctggaacga ggttatagac
     2401 atggtaatat tttcgagtta acgagcgcac cgcacccatg aggcccattg tccagatggc
     2461 gctaaaggcc aggaatatga ggacgaattg ggatgcaaaa tcaattttcc cccatacaat
     2521 cgtccctcgc ttaatggcgc ggttataaag cagataattg accaccgtca ggaatacgag
     2581 agtaaaggct gccgcattct tcgccggcat gagtgctaat tcactcgccc aagaaggcag
     2641 ctccagttcc tcggtggcca gcccttgagt cgggacaaaa ccatgagggg tcatccaaat
     2701 agcatcggcc accacaatca tcaaaaagcc gattttgatc atggtgaatg ccgacaccgt
     2761 gaaattcttg aggccaggaa ctttaccaag aataatagga aggacgacta ttatcactaa
     2821 gacaattaac gattgccatt caggcggagg gaacattttc catgtaaatc ccatgatggc
     2881 tggcatgagc agcacaacta ccgcgacaaa tccggatatt cgtacctgtt caactccctg
     2941 aattcgcttt agacttagcc atatataata gttactggcc aggaagatga gtccgaccat
     3001 ggcaccttgc atttcaaaga acatggacaa ctgatcagcc atcatgtagg gacaaatcga
     3061 agcatcataa tcacacaact cgtaggctag gagataaccc atgaaaggaa gaagcaagag
     3121 cgctcctaca ccaatcatgt tacccacaaa tcccatccag tcgtaatagg cccgctcctc
     3181 atctgttttt gaccccataa acatataagc cgcaattagc ccggcaataa aacccccaaa
     3241 cgtcacattt ccaaccaggc gatgcaggtt gaggggcatc cagctaaaat ttgccatttt
     3301 gtcccagagg ctggccgttt ggatgaactc tacgagggat aaatctcctg ccgctttagc
     3361 cggggtattc atgaaagacg ttggaccgtc aatgacaaag ag
//
LOCUS       DATIAL010000545         3791 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_849291, whole genome shotgun sequence.
ACCESSION   DATIAL010000545 DATIAL010000000
VERSION     DATIAL010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849291"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VLA60_16630"
     CDS             <1..552
                     /locus_tag="VLA60_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11039.1"
                     /translation="QEQLRDAQAQMAELANSPKKDGVRIKTLEQEHRKLEGENKWLRS
                     QRDQMRKTLTLHQIGGQSQELPFPFSSASEIIEGALTKNGYSILASMQTDQKAVYITD
                     RKTSLPPSLELSGFRNQYLLSIEKGPSDHTTIWVRAEFEKLSKNGQMFAAQQSEITDI
                     ELRLLQEIHQALSTGAAAQAKNF"
     gene            complement(662..2167)
                     /locus_tag="VLA60_16635"
     CDS             complement(662..2167)
                     /locus_tag="VLA60_16635"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HSF11040.1"
                     /translation="MKKRLSYSTDPSRREAIHEEFLRRAGRIPFLGVGLSVDVYSPDV
                     FDLYQELRREQIPMGYLEIFHATSEALEMVRARLPDMPLAYHAEGIWVTQPDWDTAYN
                     SQERLRAIAFNLRMLQAHWVNQECAAKEIAGFSFGTYLPPIFTRASADVTAYQAWNVQ
                     LQLDEYAWGPQANSPLLLLEGPPLTYFLMGEMPYAEFFSNITAIAPCGLVLDLGHVWT
                     VYRYSGAWRNQGLESFFEDFLEQFPLERVIQIHIAGLDCHPHILTQMRSGPFESPPDW
                     IDAHEAPIPDELLMLLARVIREPRLFNLKGIALEVDNKQIPLICREMKTVLQMMEPFV
                     PVPARVPLPATNLQKTETFPVENVEPSRETRDVLTGQYREYMALVSEVLSGRLDVPSQ
                     WDAEMNKGLHVYATQYLPYEILSWGGDVRVMFPNACAILDKYGISLKQFVEFWFAHPR
                     TSESEYDFFLLKIDVFVEFIGEVFPGASSLVKQEAEFLAQGYLLASQGLST"
     gene            complement(2430..>3791)
                     /locus_tag="VLA60_16640"
     CDS             complement(2430..>3791)
                     /locus_tag="VLA60_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HSF11041.1"
                     /translation="VLDWKELYDPFPDEKLKVQGARCMDCGVPFCQSPAGCPVENLIP
                     EWNDLVHRGRWKDALKALHATNNFPEFTGRLCPAPCESACVLGINSDPVSIRVIEWNI
                     IDKGFEEGWVEPVLPVGSSGKRVAIIGSGPAGLAAAQQLARTGHEVTVLEKADRIGGL
                     LRYGIPDFKMEKWVLDRRLEQMRKEGVTFQTNVCVGVDATVQDLRHDYDAVLLAMGAE
                     QPRELPVPGRDLKGVHLAMDYLTQQNKRVAGDTVPGEHISAQGKRVVIIGGGDTGSDC
                     LGTAHRQGCLEAHQFELLPQPPPTRAVSTPWPLWPMQLRTSHAHEEGCDREWSIATTK
                     FSGENGHLTSLHATRVNFEDGKVVPGSGSEIKMDVDLVLLAMGFVGPVKNGLLDSFGL
                     QYDPRGNVAVDEHFMTNVDGVFATGDTKRGASLIVWAIAEGRKSAAGIHRYLQAGQSF
                     RAS"
BASE COUNT         1060 a         1042 c          832 g          857 t
ORIGIN      
        1 caggaacaac tccgggacgc acaggctcaa atggccgaat tggcgaatag ccccaaaaaa
       61 gatggagtca ggattaaaac tttggaacaa gaacaccgaa agttggaagg agaaaacaaa
      121 tggctcagga gccaacggga ccagatgagg aagaccctga cccttcatca aattggcggt
      181 caaagtcagg agttgccgtt tcccttttct tcggcttcag aaatcataga aggcgcactc
      241 acaaaaaatg gctattccat acttgccagc atgcaaacag atcaaaaggc cgtctatatt
      301 actgatcgca aaacgtctct tcctccttcg ctggaactat caggttttcg caaccaatac
      361 ctcctctcga ttgagaaagg accttccgat cataccacta tctgggtccg tgccgaattt
      421 gaaaaacttt ccaaaaacgg gcagatgttc gcagcccagc aatctgaaat caccgacatc
      481 gaattacgcc tccttcagga aattcatcaa gcgctgtcta ccggagcggc cgcccaggcc
      541 aagaactttt aatttcctga caaacagaat ctctcaaccc agcattcaac cgatctgtcc
      601 aaactccttc tgtcgatcag gtcactgaca gacagaacca atttgccggg cacagtcgtg
      661 gttacgtcga tagcccctga gacgcgagga gatagccttg agcaagaaac tcggcttcct
      721 gtttgaccag tgaactcgcc ccaggaaaca cctcaccaat aaattccaca aatacatcaa
      781 ttttgagcag gaagaaatca tattcggatt cagatgttcg aggatgggca aaccaaaatt
      841 ccacgaattg tttcaaggaa atcccatatt tgtccaaaat agcacacgca ttgggaaaca
      901 ttaccctcac atctccaccc catgagagaa tctcatatgg taaatactga gtcgcataga
      961 catgaagccc cttattcatt tcagcatccc attggctagg aacatccagc cggccactca
     1021 ggacttcact gaccaatgcc atgtattctc tgtattggcc agttaataca tcacgagttt
     1081 cccgagaagg ctcgacgttt tctactggaa aggtttctgt cttctgaaga tttgtggcgg
     1141 gcaaaggcac acgggcaggc acaggcacaa agggctccat catctgcagt accgttttca
     1201 tttcccgaca aatgagcggt atttgcttat tatcgacttc taaggcgatg cctttcagat
     1261 tgaatagacg aggttccctt atcacccgag ccagaagcat aagtaattcg tccggaatgg
     1321 gagcttcatg agcgtcaatc caatccggag gactctcaaa tggtcctgac cgcatttgtg
     1381 taagtatatg tggatggcaa tccagacctg ctatatgaat ttggatcacc ctctccaggg
     1441 gaaattgctc aagaaaatct tcaaaaaatg attccaggcc ctggttccgc caagcgcccg
     1501 aatatcgata gaccgtccaa acatggccta gatccaggac caacccacag ggcgctatgg
     1561 cggtaatgtt cgagaaaaat tctgcatacg gcatttcacc cattaaaaaa taggttaatg
     1621 gtggaccctc caaaagcagc aatggcgagt tagcctgtgg cccccaagca tactcatcaa
     1681 gctgcaactg aacgttccac gcctggtaag ccgtaacatc ggcagaagct ctggtaaaga
     1741 taggaggcaa ataggtcccg aatgaaaaac cggctatttc tttggccgca cattcttgat
     1801 tgacccaatg agcctgtaac attcgaagat tgaaggcgat agcccgtaat cgctcttgag
     1861 aattgtatgc ggtgtcccaa tcaggttgcg tgacccatat cccctcggca tggtaagcca
     1921 aaggcatgtc aggaagtcga gctcgaacca tctccaaagc ttcagaagtc gcatgaaaaa
     1981 tttcaagata acccataggg atttgctcgc gtcgaagttc ctgatataag tcaaagacat
     2041 caggagaata cacatccacc gataagccaa cacccagaaa tggaatcctg cctgcgcgcc
     2101 gaagaaactc ttcatggata gcctctcgac gtgatgggtc ggttgaatag ctcaaacgtt
     2161 tcttcatttc agggacttaa gaggctagga aggaataaat tccgaggagg acattcaaaa
     2221 aatgcccgtc cggcaagctc aaaattacaa gaacgaagaa gtataggaag gaggcgtgat
     2281 cacagccgcg tggcgaacgc gctggggttg ccattgttca ctactttttc tttattgtca
     2341 gtagcagaac actcacaccc atcgcttaca tttcacaaga gccttctaag atcagtcgaa
     2401 ggcgcaaaac ctccgggaag gacatgaaat taggacgctc gaaaggattg tcccgcttgg
     2461 agataccggt gaatgccggc cgcagacttt cttccttctg caattgccca gacaatcagt
     2521 gacgcacccc gcttcgtgtc tccggtggca aacaccccat ccacattagt catgaagtgt
     2581 tcatcaacgg cgacattccc ccggggatca tattgaagcc caaaagaatc caggaggccg
     2641 ttcttcaccg gcccgacaaa ccccatcgcc aacaacacca ggtcaacatc catcttgatc
     2701 tcggaccccg acccaggaac caccttccca tcctcaaaat tcacacgggt cgcatgcaaa
     2761 ctagtcagat gtccgttctc ccctgaaaat tttgtggtgg caatactcca ttcacgatca
     2821 cacccttcct cgtgggcatg agacgttcgt aattgcatcg gccataaggg ccatggagtc
     2881 gacacagccc gagtcggtgg cggttggggt aacaactcga attgatgggc ttccaggcac
     2941 ccttgacgat gagccgttcc cagacaatct gaaccggtat caccaccacc aataatgacc
     3001 acccttttgc cttgagccga tatgtgctct ccaggaacag tatccccggc cactcgctta
     3061 ttctgctggg tcagatagtc catggcgaga tgaacgcctt taaggtctct acctggaacc
     3121 ggcaattctc tcggttgctc agctcccatt gcaagtaaca cggcatcata gtcatgacgg
     3181 aggtcctgaa cggtggcgtc cacacccaca cacacgttgg tctgaaacgt cacaccctct
     3241 tttcgcattt gctccaatcg acgatctaat acccacttct ccattttgaa atccgggata
     3301 ccatagcgaa gcaagcctcc aatccgatca gccttctcca aaactgtcac ttcatgaccc
     3361 gtccgggcta attgttgtgc tgccgccagt cctgctggac cagacccgat aatcgcaacg
     3421 cgcttcccgg aagagccaac cggtaacacc ggttcgaccc aaccttcctc aaatccttta
     3481 tcaataatat tccattcaat gacacgaatg gagaccggat cgctattaat gcctaacaca
     3541 catgccgatt cacatggagc cggacatagg cgcccggtga actcaggaaa gttattggtc
     3601 gcatgaagcg ctttgagggc gtctttccat cgtcctcgat gcacgagatc attccattcc
     3661 gggatcaaat tctccacagg acagccggcg ggactttggc aaaatggcac cccacagtcc
     3721 atacagcggg ccccttgaac ttttaacttt tcgtcgggaa atgggtcata caattccttc
     3781 caatctagaa c
//
LOCUS       DATIAL010000546        16730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1063781, whole genome shotgun sequence.
ACCESSION   DATIAL010000546 DATIAL010000000
VERSION     DATIAL010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16730
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063781"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VLA60_16645"
     CDS             <1..1108
                     /locus_tag="VLA60_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HSF11042.1"
                     /translation="APPEQMPPQRQSVLIAYAWGVWVYRAVVFVGIAVLVYYFFFKVL
                     GVILFLVEIGWFLVWPVYEELQVWWTRRAAVTQSRRGRGIGVALTGCLLMSVMPLDTT
                     VEIPAILEAPERMTLFPPAPAMVVEVLVEEGDRVEHGQTLLILQSPQVEHQIDIIGHR
                     IAALELRAEREVAYQDDRDDHLVIWETLVGERKALAGLVTLREQLILRAPFSGVVTDL
                     ASSLHPGLWVNTDMAMAHVIGEHPSVMLALATEKEKARLSAGNEGWFYPDDPTRPARQ
                     GQLRDLRHVDESEMALPYLASTYSGGVPVRQDAGGRLIPEHSVYRVELDVTDKDPSWN
                     QVVRGVVHVKGNGQSVMGHLWARAASILIRESGA"
     gene            complement(1375..1623)
                     /locus_tag="VLA60_16650"
     CDS             complement(1375..1623)
                     /locus_tag="VLA60_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11043.1"
                     /translation="MNLAKSQLAFIRNHCETPVFVPLVNRSGIPLADGLQGIQEEIGY
                     EIPVVIPTASELCYTAGIKKCRSSASIPKASLPYSLSS"
     gene            complement(1712..2173)
                     /locus_tag="VLA60_16655"
     CDS             complement(1712..2173)
                     /locus_tag="VLA60_16655"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF11044.1"
                     /translation="MEIHKTVQAFQRLKGQETILQTAGVDTSEGASRCHVIGFAGAKG
                     GVGTTTVALNVAMTLIHGGHRVIFVELSPHSGTAAWLLLMPHQSPLRHASTTLTDMNE
                     AVLSQLFMQHSTGVAGPLCVHLGSRSGGSSLHGILDRVIPGTQRTRRLSGT"
     gene            2688..10343
                     /locus_tag="VLA60_16660"
     CDS             2688..10343
                     /locus_tag="VLA60_16660"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HSF11045.1"
                     /translation="MKHLHGTPATQEAGKNAKMVKPGSRKKWKSRPPQESPTAILIGA
                     CIALEPRMLFDGAALATGVEVVQDITTQDQPGVPDGKSETGTDSTTDASKSEAIWSSG
                     LSLSTPSDRKEIVFIDTRVENYQTLMDGIDPAAEVILLDARRDGIEQIAGTLKDRSDI
                     DAIHLIGEGTEAEMHLGTAFLTNDSISDHYAALFSQIGQSLSADADLLIYGCNFGRGA
                     GGLSAIQALANLTGADIAASTDRTGHVSEYANWQLEISTGFIESSIVIGQATQDAWEG
                     VLATYTVTNTNDSGAGSLRQAITDANANVGSDTINFSIAGAGPHTISLSSALPTITGA
                     VLIDGWSEPDFAGTPVVRIDGTSAGAGVDGLTFSGTSDGSTVRGLMITTFSRDGILIQ
                     SGADNITVVGNWIGTTGTGSTGVGNGDDGIEIAGSNAIIGGTGANDRNVITNSGDEGI
                     TIVGSGVTGHLIQGNYIGLDPDGSTGGGNTDVGIAIITGSGNTIGGTTAAARNVISKN
                     FEGIEINSNNNIVQGNYIGTDAGGTLNRGNRSDDGVEVQGSATGNLIGGTAAGAGNLI
                     AFNALDGVNIVNGSSNAVLGNRIHSNSNLAIDMGTSGVTANDAGDPDTGANNLQNFPV
                     LATADVDSPTQVTIDGTFNSTASTTFRIEFFKNTIATGQDPSNHGEGETYLGFTSITT
                     DGSGNGSFNLTLTASVAAGEFVTATATEDLGGGNYGSTSEFSLNETAVAENDAPVLTS
                     ASPTLTTITEDQTTNSGDLVSAIVGAKITDVDTGAVEGIAITGLTSGNGTWEYDIGGG
                     WTAVGAVSDASVLLLRATDSLRFVPDGQNADSATVTYQAWDQTSGTEGTKVNASTNGG
                     TTAFSTATDTATITVTAINDAPGITSDGGGPTAAFNRSENITAVTTVTAMDVDSPPDT
                     LQYTKIGGADAALFNLDINTGVLRFIVAPDFEAPGDANTDNVYEVTVQVSDGNGGFDT
                     QTISVTVVDNVGTLTVDTTSDIADGDTSSIAALLADKGFDGFISLREAIIASNNTGND
                     GSADQINFNIAGAGPHTISPSSALTTLTDVVVIDGYTQAGSSANTLATGNDGVLQIEL
                     NGSGAGSANGLTLGSGSAGSTIRGLIINRFSLQGIQLSSDNNVVVGNWIGLDNTGAVD
                     QGNAIDGITITGNNNTIGSAAAADRNVISGNNDEGVDIDPGISGTVIQGNYIGTNISG
                     TAAVGNGQVSVGPWGGIIVDGSNNTIGGANTGEGNLISGNNQWGVWIIGTGNRVLGNK
                     IGTDAAGTVALGNVGDGIQIATSSNKVGGVNAGEGNLIAHNTNNGVNVTGGTNNNILG
                     NQIHSNNGLGIELGSTGVTANDAGDVDAGANDLQNFPVLATADVDTPTQVTIDGTFNS
                     TASSSFRVEFFKNTAATGDDPSGHGEAQTYLGFTSVTTDGSGNASFNLTLNASVTAGE
                     FVTATATEDLGGGNYGSTSEFSLNTTAVAENDAPVLTPASPTLTTITEDDTTNSGDLI
                     SAIVGASITDVDTGAVEGIAITGLTSGNGTWQYNMGSGWTAVGAVSNTSALLLRATDS
                     LRFVPDGQNADSATVTYRAWDQTSGSAGTKVDASTNGGTTAFSTATDTATITVTAVND
                     APVNTVPGAQSTNEDTSLTFSSVNSNQISISDIDAGGNPIEVTVSVTNGALSLSGTTG
                     LTFTGGDGTADTTMTFRATVTDINTALNGLTYIPTAEYNGGATLTITSVDNTLVSLNI
                     DANLQARYTFEGNANDVAPGTAQNGTLTNGASIVTDGTRGQVLSLEGVNDFVDLSTHT
                     ADFTAFTQGTISGWIKATGTFETIFSISDTAGTGSYAALFLGASGYLTYEVMENGVMQ
                     LAVYKSDVTINDGNWHHVAVTIGPSGNSLYVDGVLATPGQLTYDTGSGSTQAFFSSVS
                     GLDSMAIGRNQDSSGGKWYTTGGLDDVRLYDRVLTSSEVEALASDLALTDVDTVAITV
                     VEVNDAPGLTPGAPTFTPITEDETTNSGDVVSALVGASITDVDTGAVEGIAITALTSG
                     NGSWEYNTGSGWTAVGAVSDASALPLRSTDSLRFVPEGQNADSATVTYRAWDQTSGST
                     GTKVDASTNGGTTAFSTATDTASITVTAVNDAPVITSNGGGPTAAVNAPENQTAVTTL
                     TATDVDVPADTLTYSISGGADAALFSLDPNSGVLRFLVAPDFDAPTDVGANNVYDVVV
                     QVSDGNGGIDTQAIAVTVTDSNDAPIITSNGAGPTAAVYISENQTFVTTVTATDVDMP
                     ANTLTYTILDGADAALFSIDPITGLLTFNVPPDFEAPTDADANNIYEVVVQVSDGNGG
                     VDPQMIQVTVTNIQEGMPQPPSPQPGPTPTPAPSPRPTPESPLDTNTSPPGAPPIGFP
                     DSFGGQPPAGRDLTRHPDPQLVPDWNRVLEVAPFLRPGTFGTTSEQIRAYTPAPVLLS
                     SIELSQEFLKQLNSFADDLKETTQQTIDERSFFIKMMEYAGVGISGVLLAWLVRSGTL
                     LASVLAMLPAWRNFDPIAILDMDQESREIFTQKMREAAEQETREHHGLDRMLDQNIDD
                     SVPPSSTFSSGSS"
     gene            10340..11854
                     /locus_tag="VLA60_16665"
     CDS             10340..11854
                     /locus_tag="VLA60_16665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HSF11046.1"
                     /translation="MKRLTPIFWMSLGLVSLTLSILLISDLMVGLIPDQAAQIFTYRQ
                     KFSEAMAVQYSILAQRGDNQGLQQALDLLVERNADIQSVALVLESGEIMAMAGPHHAL
                     WTQPSGDHSTPERIQIPIFNGDLPWGTLQLRFPSLDTGFWTRVLNNTWVRFLALVMVY
                     GFVGYFLYMKRTLRQLDPSSVVPMRVQAAFDVLAEGVVFLDGEDRIVLANRAFSLIVD
                     VAPHDLIGKTLDSFEWTSSDMGNPLNIYPWKTARQGKCLKLDVPLVWERMGETVKKFR
                     VNCTPILGQRKQVRGILVSFNDVTVLEGTIRELELSKTELEKLAHRDPLTGCCNRRAL
                     FEAFEELWERAQHDGSDLVCIMADIDHFKSYNDRFGHAVGDQVIELVAKLLSTALRPL
                     DIIGRYGGEEFCILLPGQTSVEGMLVAERLRETIELSASTAIRTTSGQKITMSFGVSA
                     AALGASDYLELVAQADKALYVAKENGRNRVEQWMVNEAVAASAQDEKLMSHSVS"
     gene            11948..12208
                     /locus_tag="VLA60_16670"
     CDS             11948..12208
                     /locus_tag="VLA60_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11047.1"
                     /translation="MDKGIFSGRKKSVSVVPWGAFMIDLFLTIFGTAVVLILSFIAPI
                     FLLTGELFLGILSLGYYRPQLKKEALKSELPYRERSVFGLVY"
     gene            complement(12337..12825)
                     /locus_tag="VLA60_16675"
     CDS             complement(12337..12825)
                     /locus_tag="VLA60_16675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HSF11048.1"
                     /translation="MNTGMHPYEGWDAFQTHIRLATNAVEKKYPPPYLSRIWLHFRNI
                     IRRSALGLTDKDWNQLLQNKWTGDLAWPEMAGAMKATHSEVVMGLNGGDDLVCVRHKI
                     VPIAQPIQEMGYLIDHDFFVNGQFSMTEISRKLTEYHQAAQRFFKLWITDMLHQAMKP
                     AT"
     gene            complement(12868..13734)
                     /locus_tag="VLA60_16680"
     CDS             complement(12868..13734)
                     /locus_tag="VLA60_16680"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HSF11049.1"
                     /translation="MLTAEELVKNCTKLFTLPEVYLQVKKVIDNPESTMADLSRAISI
                     DPGMTAAVLKLVNSAFYAMPRKVETISRAVGILGMQPLHDLTLAISVTKTFSGLNQHV
                     MSMSVYWSNSFFSGLVARELARKCFLVDSERMFVEGLLRDIGHLLLYEQLPEPSEQVL
                     RESAKDGSPVHILEQQLLGFDYTEVGQTLIEEWQLPKNFGIAIRYQNNPSGTSEHGFE
                     AALLNMASALTEGFQSPKGHAQWQNLVAPESWKLTGLDEEGLKECMLEAGKQLSGMLT
                     LMEEARLPVGAS"
     gene            13825..13986
                     /locus_tag="VLA60_16685"
     CDS             13825..13986
                     /locus_tag="VLA60_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11050.1"
                     /translation="MNFFRSGLAVGPWNWAGLVPKRLVGGECGREAVPGLSDQAAVIL
                     VPQRGMWEE"
     gene            complement(13987..15384)
                     /locus_tag="VLA60_16690"
     CDS             complement(13987..15384)
                     /locus_tag="VLA60_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 family protein"
                     /protein_id="HSF11051.1"
                     /translation="MKLKPGSPGKGILRQMHKAQEKYLEGWLDVPAHVHHTAYRMANP
                     AVERPQSRKEFQQLLACLEIDPTHAVMEDKVGFGVKKGTNGDKLIVIWEAHTEYYSYQ
                     VWHIPSNATQPLGFGPLGFSQYVFPFSPLGIQVNALDLLVIPARTYDQEELGTLLPGP
                     QLYASKVLGEDICVATSFTPDEHDRERYLIWAPDPGLLRQKIARLMTILTTLENYTHL
                     ILLPYPAFSRSVDQVQIFEQRHLYQRTLITKELNRATHSTLQQWLEGLTHDFLEVGRL
                     TASLRYRLSASVPYDRIVHSNTLALQEQPLSYGRTLIDYFQWKITGVADGYQQLLSRV
                     QALEQDFEGTIAVLRTKVELLLQEQNLAQQDQNVNLLASLDKTTKSQVVLQHTVEGLS
                     VIVIAYYVSGLANYLFKALHELGWLDKYELASGIFVPIALGLSFAIITWGRNRIQKKI
                     TAMPPGSTSHPSKHA"
     gene            complement(15562..16500)
                     /locus_tag="VLA60_16695"
     CDS             complement(15562..16500)
                     /locus_tag="VLA60_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HSF11052.1"
                     /translation="MTLLPRWWPRGSFPSGVPTQDRIFQVSTEVGILTKCHWQPSPSQ
                     HPTILMVHGLEGCTESHYMRGLARKCWDAGWNCIRINQRNCGGSEHLTPTLYHNGLSQ
                     DYGRIIQEITEEDGCTAVWLIGYSMGGNLALKLAGEHGSTLPSLRGVAAVCPNIQPAA
                     CVRALQRPSNWLYHRHFLKSLTGKLRKKARLFPGRWDLSHLSHIRTMWEFDEIYTAPD
                     GGYRDAEDYYEQSAARNTLSSITIPTLVITAQDDPFIPYHMFADPALDANPFIQLEAP
                     AHGGHCGFFQRHQNHEDPFWAENRLCDWIHAQLNSA"
BASE COUNT         3849 a         4076 c         4930 g         3875 t
ORIGIN      
        1 tgctcctccg gaacagatgc ccccgcaacg gcaatccgtg ctgatcgcct atgcgtgggg
       61 ggtgtgggtc taccgggccg tcgtgtttgt cggcattgcg gtcctggtgt attacttttt
      121 tttcaaggtt ttgggcgtca tcctctttct cgtagaaata ggctggtttc tggtatggcc
      181 ggtctatgag gaactgcagg tctggtggac acgtcgggcg gccgtgaccc aatcccggcg
      241 agggcgggga attggagtcg cactgacggg gtgtctgctg atgagcgtga tgcccttgga
      301 tacgacagtg gaaattcccg cgattcttga agctccggaa cgcatgaccc tgtttccacc
      361 ggcaccggca atggtggtgg aggtgttggt tgaggaggga gatcgggtcg aacatggtca
      421 aacactttta attttacagt cgccgcaggt ggaacatcag attgacatta tcggacatcg
      481 cattgcggcg ttagaattac gcgcggaacg agaagtcgcc tatcaggatg atcgggatga
      541 tcatctggtg atttgggaga cgctggttgg cgaacgaaag gcgctggccg gcctggttac
      601 gcttcgggag caattgatct tgcgtgctcc gttctccggc gtggtcacgg atctggcgtc
      661 gtcactccat ccagggctgt gggtgaatac cgacatggcg atggcccatg tgattggtga
      721 acatccatcc gtgatgctgg ctctcgccac ggaaaaagaa aaagcccggc tctcggcggg
      781 gaatgaagga tggttttatc cggacgatcc tacgcgacct gcccgacagg ggcagcttcg
      841 ggaccttcgt catgtggacg aatcggaaat ggcgttgccg tatttagcct ccacctattc
      901 cggaggtgtg cccgtgcgcc aggatgcggg gggcagattg attcccgagc attccgtgta
      961 ccgggtggaa ctggatgtga cggacaagga tccatcctgg aatcaggtgg tgcgaggggt
     1021 ggtccatgtg aagggcaatg ggcaaagtgt gatgggacat ctctgggcac gggcggcctc
     1081 cattctcatt cgggaaagcg gcgcctgatt gttggaagga aacgtggggt tggggagatg
     1141 cgctcgctta agcgggaggg tctttgatca gagagtggac tcatccatgc gtagatgaca
     1201 agaggctgga gcggcagttg tgtgaagtcc tcttttccag aactttccat agaaagggga
     1261 gcagaggtta ccagacacca cggtctcgac ggttgccttt ccggcggtcc ccgcctcgtc
     1321 ggtctcgttt gggctccgga ccgtctccgg aaacgtagcc gagcagttgt tcgctcagct
     1381 tgacaaactg tatggcaagg acgctttcgg gattgaagca gacgatcgac atttttttat
     1441 gccggcggta taacataact ccgaggccgt ggggatgacg accgggattt catagccgat
     1501 ctcttcctgg ataccctgaa gcccgtcagc cagtggaata cccgagcgat tcacgagggg
     1561 aacaaaaacg ggagtttcgc agtgatttcg gataaacgct aattggcttt tcgctaaatt
     1621 cataccaatg gcatcgctct ccatcacgaa atcaaggatc tgacattgac ggagaaaaca
     1681 catggatgga aaagagggct ccaaaggaaa atcaagtacc agatagtctg cgagtccttt
     1741 gagttcccgg aataacacgg tcaagaattc cgtggagact tgagccccca cttcttgatc
     1801 ccaggtggac acacagagga cctgcaaccc ctgtcgaatg ttgcatgaac agttggctca
     1861 gaacagcttc attcatgtct gttaacgtgg tcgatgcatg gcgcaaaggc gattggtgtg
     1921 gcatgagaag aagccaggcg gcagtccctg aatgggggct cagctcgaca aaaatgacgc
     1981 ggtggcctcc atgaataagg gtcatggcga cattgagtgc caccgtggtc gttccgactc
     2041 ctcccttggc accggcaaag ccgatcacat ggcagcgtga tgctccctcg cttgtatcga
     2101 cgccggccgt ttgtagtata gtttcctgtc ctttcaatcg ctgaaacgct tgcacggttt
     2161 tatgaatctc catccccatt tccctctcta tggtagtttc ggaatgtctg tggtttgtca
     2221 cccggtcaac gtgtccgtag gggtttggtg ttgtggtgag atgtctgaac tgattcccct
     2281 ggatgtgagg gtgtgccttt gtatcgcaaa aagcgtgaat gctcttaagg aaaaaagcat
     2341 acgatggcaa gccggttccc tatgccttcc ggagaggctg aagagactga tcgatatctg
     2401 gaatgacgtg gtattcaaca acaattcccg caacccaact tgcactatgt tttcgatacg
     2461 tgacagaaca tccaccaaca ttcagtagcc ggaaaataac cccctacaac cgttcttaat
     2521 ccgcatcaag agtcgttccc caaggcccga caaaggatac aggaacttct ccttttttaa
     2581 ggggacgact ctgttaatcg atgacctatg ggtatcaagg caatttaaac attttgtgac
     2641 ccgagactat tcgtcatccg gtttctctca tgattgaaga tcgtcacatg aaacatctgc
     2701 atggcacgcc cgcgacgcaa gaagcgggga agaatgccaa gatggtgaaa cctggttccc
     2761 gaaagaagtg gaagtcccga ccaccccagg agtcccccac tgccatattg atcggagcct
     2821 gtattgcctt agagccacgg atgctattcg atggagccgc ccttgctacg ggagtggaag
     2881 tcgttcaaga catcaccacg caagatcaac cgggcgttcc cgatggcaag agtgagaccg
     2941 gtacggattc caccaccgat gcttccaaga gtgaggccat ctggtcctcc ggtctttccc
     3001 tctcaactcc gtctgatcgg aaagaaattg tcttcatcga tacgcgtgtt gaaaattacc
     3061 agacgcttat ggacgggatc gatccggccg cggaagttat tcttctggat gcgagacggg
     3121 acgggatcga acaaattgcg ggaaccctca aggaccggag cgatatcgac gccattcatc
     3181 tcatcgggga aggcaccgag gccgagatgc atttgggcac ggcctttctc acgaacgatt
     3241 cgatcagcga tcactatgcg gcgctgttca gtcagattgg gcagagtctt tctgcggacg
     3301 ccgatctgtt gatctatggc tgtaacttcg ggcgtggagc aggcggcctt tccgctatcc
     3361 aggcgttagc aaatctcacg ggagcggata tcgccgcgag taccgaccgg accgggcacg
     3421 tgagcgaata tgccaactgg caactggaaa tttccactgg gttcattgag tcgtccatcg
     3481 tgatcggcca ggccacccag gacgcctggg aaggtgtgct ggccacgtat accgtgacca
     3541 acacgaacga ttcgggggcc ggatcccttc gccaagccat cacggatgcc aatgccaatg
     3601 tcgggagtga caccattaat ttcagcattg ccggtgcggg tccccacacg atatccctaa
     3661 gttcagccct accaactatt accggtgctg tgctcatcga cgggtggagt gaacccgatt
     3721 tcgccggaac gccggtggtg aggatagacg gaacatctgc cggggcgggt gtggatggct
     3781 taaccttcag cgggacgagc gatggcagta cggttcgcgg attgatgatt accacgtttt
     3841 ccagggatgg gattctcatt cagtcgggtg ctgacaatat cacagtggtt gggaattgga
     3901 tcgggacgac cgggaccggt tcgaccggcg taggaaatgg agatgatggg attgaaattg
     3961 ctggatccaa cgcgatcatc ggcggcacag gggccaatga tcgaaacgtc attaccaaca
     4021 gtggtgacga aggcatcaca atcgtgggtt caggagtcac cggacatctc atccaaggga
     4081 attacatcgg tcttgatcct gacggatcaa ccggcggggg caatacagat gtcggtattg
     4141 ccatcataac ggggtccggc aatacgattg gcggtacgac agcggctgcc agaaatgtca
     4201 tttcaaaaaa tttcgaaggg atcgaaatca actcgaataa taacattgtg cagggaaact
     4261 acatcgggac ggatgcgggc ggcacgctga atcgaggcaa tcgatcggac gatggagtgg
     4321 aagtccaggg cagtgcgact ggcaacctga tcggcgggac ggcagccgga gccggcaacc
     4381 tcatcgcctt caacgcattg gatggggtga atatcgttaa cggaagtagt aatgcggtac
     4441 ttggaaatcg aattcattca aacagtaact tggccattga catgggtacg agcggagtca
     4501 ccgctaacga tgcgggtgat ccggacaccg gcgccaacaa cttgcaaaat tttcccgtgc
     4561 tggcgacagc cgatgtggat tcccccacgc aggtcactat cgatggaacc ttcaacagta
     4621 cggccagtac cacttttcgc attgagttct ttaaaaacac gattgccaca ggacaagatc
     4681 catcaaatca cggcgaaggt gaaacgtacc tgggctttac gagcatcacg acggatggaa
     4741 gtggcaacgg aagttttaat ctcacactga ccgcctcagt tgctgcgggt gaattcgtga
     4801 ccgccacggc caccgaagat cttggcggcg gcaattatgg ctccacttcg gaattttccc
     4861 taaatgaaac ggcggttgct gaaaacgatg cgccggtctt gacgtcggcg agtcccacgc
     4921 tcaccaccat cacggaggat cagacgacga atagcggcga cctggtatcg gccatcgtgg
     4981 gggcgaaaat cacggatgtg gatacggggg cggtcgaagg gattgccatc accggcctca
     5041 cgagcggcaa tggcacatgg gagtacgaca tcgggggcgg gtggacggcg gtaggggcgg
     5101 tcagtgatgc ctcggtcctc ctgctccggg ccaccgatag cctgcgcttt gtcccggacg
     5161 ggcagaatgc ggacagtgcg acggtgacct accaggcatg ggatcagacg agtgggacgg
     5221 aagggacgaa ggtgaatgcg tcgaccaatg gcggcaccac cgcgtttagt actgcgacgg
     5281 atacggcgac gatcacggtg acggcgatca atgatgcgcc gggcattact agtgacggtg
     5341 gagggcccac tgcggctttt aaccggagtg agaacatcac cgcggtgacg acggtgacgg
     5401 cgatggacgt ggattcgcca cccgatacct tgcaatacac caaaatcggc ggggcggatg
     5461 cggcgctgtt caatttggat atcaatacgg gggtgttgag gttcattgtg gcgccggact
     5521 tcgaggcgcc cggggatgcc aacacggaca atgtgtatga agtgaccgtg caggtcagtg
     5581 acgggaacgg tggattcgac acgcaaacca tctccgtcac ggttgtagac aatgtcggaa
     5641 cgttgacggt cgatacgaca tctgatatcg ccgacgggga tacctcctcg atcgcagcct
     5701 tgctggcgga taagggattt gatgggttta tttctcttcg agaagccatt atagccagca
     5761 ataacacggg taatgatggt tcggcggacc aaatcaattt caatattgca ggggccggcc
     5821 cgcatacgat cagtccgtct tcagctttaa ccacccttac cgatgtcgta gtgattgacg
     5881 ggtacactca agccggttca tcagccaata cccttgcaac tggaaatgat ggtgttttgc
     5941 aaatagaact gaatggatcc ggtgcgggta gtgcaaacgg actgactctt ggctcgggga
     6001 gcgcaggcag taccatcagg gggttaatca tcaatcgctt cagcctccag ggtattcaac
     6061 tcagtagtga caacaatgtc gtagtgggca attggatcgg cttggataat accggggcgg
     6121 ttgaccaggg gaacgccata gatggtatta cgatcaccgg gaataataat accattggca
     6181 gtgctgccgc tgcagatcgc aacgtgatct ccgggaacaa tgacgagggt gttgatattg
     6241 atcctggcat ttccgggaca gtcatccaag gaaactatat cggcaccaat atttcaggca
     6301 ctgcggctgt cggcaatgga caggtcagtg tggggccatg gggaggaatt attgtagacg
     6361 ggagtaacaa cacgattgga ggcgcaaata cgggggaggg aaacctcatt tccggcaata
     6421 atcaatgggg tgtgtggatc atcgggacgg gaaatcgcgt gctgggcaac aaaatcggca
     6481 ccgatgccgc gggaactgtc gctttgggaa atgtcggcga tggcattcag atcgccacct
     6541 cgagtaacaa ggtgggtggg gtgaatgccg gcgaaggcaa tctcatcgca cacaacacca
     6601 ataatggtgt gaacgtcacc ggagggacga acaataatat tcttggaaac cagattcatt
     6661 ccaataacgg tctgggcatt gagttaggaa gtaccggagt taccgccaac gatgcgggtg
     6721 atgttgatgc gggggccaac gacctgcaga attttcctgt gctggcgacc gccgatgtgg
     6781 ataccccgac tcaggtcact atagacggga ccttcaatag tacggccagt tcctcgttcc
     6841 gggttgagtt tttcaagaat accgctgcga ccggcgatga tccttccggt cacggagaag
     6901 ctcaaaccta tctcggtttt acgagcgtca cgacggatgg aagtggcaat gcgagtttta
     6961 atctcacttt gaacgcctcg gttacggcgg gtgaattcgt gaccgcgact gccaccgaag
     7021 atctgggcgg tggtaattat ggttcaacct cggaattttc gcttaacact actgcggtag
     7081 cagaaaacga tgcgccggtg ttgacgcccg ccagtcccac gctcacgacc atcacggaag
     7141 atgacacgac gaacagcggt gacctcatct cggcgattgt cggcgcctcg attaccgatg
     7201 tggacacggg ggcggtggag ggcattgcca tcacgggcct gaccagtggc aacggcacgt
     7261 ggcagtacaa catggggagc ggctggacgg cggtgggcgc ggtgagcaat acgtccgcgt
     7321 tgttgttgcg cgcgaccgat agcttgcgct ttgtgccgga cggacagaat gcggacagtg
     7381 cgacggtgac ctaccgggcg tgggatcaga cgagcgggag tgccgggacg aaggtggatg
     7441 cctcgaccaa cggcgggacc acggcgttta gcaccgccac cgatacggcg acgatcacgg
     7501 tcacggcggt caatgatgcg ccagtgaaca ccgtgcccgg ggcgcaaagt actaacgaag
     7561 ataccagtct gaccttctcg tccgtgaaca gtaatcagat ctccatcagt gatatcgacg
     7621 ccggcggcaa tccgattgaa gtcacggtct cggtaaccaa tggcgcgctc agtctatcgg
     7681 gcacgacggg tctgaccttt acgggcggcg atgggacggc ggatactacc atgacgttcc
     7741 gtgccacggt gacagatatc aacaccgccc tcaatgggct gacctatatc ccgacggcgg
     7801 agtacaacgg gggcgccacc ctgacaatca cgtcggtgga caacaccctc gtgtcattga
     7861 atatcgatgc gaatcttcaa gcgcgctata cattcgaagg caatgccaac gatgtggcgc
     7921 cgggtaccgc tcaaaacggg acgctgacca atggcgcatc gattgtcacc gacggaacaa
     7981 gaggacaggt cctgagcctg gagggagtca atgactttgt cgatctatcg actcacacgg
     8041 ctgacttcac tgcgtttaca caaggcacca tcagcggatg gattaaagcg acgggcacct
     8101 ttgaaaccat cttttccatc tccgacaccg caggcacagg gagctacgcc gctcttttcc
     8161 tgggagcttc agggtatctg acctacgagg taatggaaaa cggcgtgatg caactggcgg
     8221 tctacaaaag tgatgtcacc atcaacgacg gtaattggca tcatgttgcg gtgacgatcg
     8281 gcccatccgg caacagtctc tacgtcgatg gagttctcgc cacgcctggg cagttgacct
     8341 acgacacggg aagcggttcg acgcaagcct ttttcagcag cgtgtccggg ttagattcca
     8401 tggctatcgg acggaatcaa gattcgtccg gtggtaaatg gtacacaact ggtggcctgg
     8461 acgacgttcg tctctacgac cgagtcttga cgtcctcgga ggtagaagcg ctagcgagcg
     8521 atctagccct gaccgacgtc gataccgttg cgattacagt ggtcgaggtc aatgatgcgc
     8581 cggggttgac gccgggggca cccacgttca cccccatcac tgaagatgag acgaccaata
     8641 gcggcgacgt ggtgtcggcc ctcgtgggcg cgagtatcac cgatgtggac acgggggcgg
     8701 tggagggcat cgccatcacg gccctcacct cgggcaacgg ctcgtgggag tacaacacgg
     8761 ggagcggctg gacggcggtg ggcgccgtca gtgatgcctc ggccctgccc ctgcgcagta
     8821 ccgatagctt gcgctttgtg ccggaggggc agaacgcgga cagtgcgacg gtgacctacc
     8881 gggcgtggga tcagaccagc ggcagtacgg ggacgaaagt ggatgcgagt accaatggcg
     8941 gcacgacggc gtttagtacc gccacggaca cggcgagcat taccgtgacg gcggtcaacg
     9001 atgcgccggt gattaccagt aatgggggag ggccgaccgc ggccgtcaat gcaccagaaa
     9061 accaaacggc cgtgacgacg ttgacggcga ccgatgtgga tgttccggcg gacaccttga
     9121 cctatagcat cagcggtggg gcggatgcgg cactgttcag cctggatccc aacagcgggg
     9181 tcttgaggtt ccttgtcgcc cccgactttg atgcgccgac cgatgtgggt gccaacaacg
     9241 tctatgacgt ggtggtacag gtgagtgacg gtaacggcgg tatagatacg caagcgattg
     9301 ccgtcacggt gacggatagc aacgatgcgc cgatcatcac gagtaatggg gcagggccga
     9361 ccgcggcggt ctatatatca gaaaaccaga cgttcgtgac gaccgtgacg gccacggatg
     9421 tagatatgcc ggcaaatacg ttgacctata cgattttaga cggggcggat gcggcgttat
     9481 tcagcattga tccgatcacc gggctgttga ccttcaacgt gcccccggac tttgaagcgc
     9541 ccaccgatgc cgatgcgaat aatatctatg aggtggtggt acaggtgagc gatggtaacg
     9601 gcggtgtgga tcctcaaatg attcaggtga ccgtcacaaa tatccaggaa ggcatgcccc
     9661 aaccgccctc gccgcaacca ggtcccacgc ccacgcctgc gccaagccct cgtccaactc
     9721 cggagtcgcc gcttgatacc aatacttcgc ctccaggtgc acccccgatc gggtttccgg
     9781 attcattcgg tggtcaacct ccagcgggac gggatcttac ccgtcatcct gatcctcagt
     9841 tagttcctga ctggaataga gtcttggagg tcgccccatt tctgcgtcca ggcacttttg
     9901 gaaccacctc agaacaaatt cgtgcctata ctccggcccc ggtgctgctt tccagcattg
     9961 agctgagcca ggaattcctt aagcagctga actcttttgc cgatgatttg aaagagacca
    10021 cccaacaaac cattgatgaa cggtcctttt tcatcaagat gatggaatat gcgggggtgg
    10081 gcatatcggg agttcttctg gcgtggctgg tgagaagtgg cacgctcttg gcgagcgtgt
    10141 tggcgatgct cccggcctgg cggaactttg accctattgc aattttggat atggatcaag
    10201 agagtcggga aatcttcaca cagaaaatgc gggaagccgc agagcaggag acccgggagc
    10261 atcacggatt agatcggatg ttggaccaga atattgatga ttcggttccc ccttcctcca
    10321 cgttttcctc cggatcctca tgaaacgact gacacccatc ttctggatga gtctggggct
    10381 ggtatccctg accctgagta ttctcttaat cagtgacctg atggtgggcc tcattccgga
    10441 ccaggctgct caaatcttta cctaccggca aaagtttagc gaagcgatgg ctgtccagta
    10501 ttccatcctg gcgcagaggg gtgacaatca gggcctccag caggccttgg atctcctggt
    10561 tgaacggaat gcggatatcc aatcagtggc tttggtcttg gaaagtggtg aaattatggc
    10621 catggccggg ccccatcatg ctctctggac ccagccatca ggggaccact ctacgcctga
    10681 acgcattcag attcccattt tcaacgggga cctgccttgg ggaaccttac aactccgatt
    10741 tccttcactg gatactggat tttggacccg tgtgcttaat aatacctggg tgcgatttct
    10801 tgcgctggtc atggtctacg ggtttgtggg ctattttttg tatatgaagc gaacgttgcg
    10861 gcaactcgat cccagttccg tggtacccat gcgggtgcaa gccgcgtttg atgtgttggc
    10921 agagggtgtg gtgttcctgg atggtgagga ccgtattgta ctcgccaatc gcgcctttag
    10981 cctcattgtc gatgtggctc cccacgacct gatcgggaaa acactggata gttttgaatg
    11041 gacgtcatcc gacatgggta acccgttgaa catctaccca tggaagacgg cgcgacaggg
    11101 aaaatgcttg aaattggatg tgcccctggt ttgggagagg atgggggaaa ccgtgaagaa
    11161 gtttcgagtc aattgcactc ccatcttggg tcaacgcaag caggtccggg gtattttggt
    11221 atctttcaat gatgtgacgg tattggaagg cacgattcgg gagttagagt tgtcaaaaac
    11281 cgaattggag aaactggctc accgagatcc cttgacgggt tgttgtaacc ggcgagcgtt
    11341 attcgaggcg tttgaagaac tgtgggagag ggctcaacat gatgggagtg atttggtctg
    11401 tattatggcg gatatcgacc atttcaagtc gtataacgat cggtttggcc atgcggtggg
    11461 ggatcaagtg atcgaactcg tggcgaaatt gttatcaacc gctttgcgtc ctttagatat
    11521 tattggccgg tatgggggag aggaattttg cattctcctg cctgggcaga cttctgtcga
    11581 gggcatgttg gtggccgaac ggttacggga aaccattgaa ctttcggcca gtacggctat
    11641 tcgcacgacg tccgggcaga agattacgat gagttttggt gtgtcagccg ccgctttagg
    11701 agcttctgat tatttggaat tggtggctca agccgacaaa gccttatatg tggccaaaga
    11761 gaatgggcgg aatcgggttg aacagtggat ggttaatgaa gcggtcgctg cgtccgctca
    11821 agatgagaaa ttgatgagtc actcagtatc ctagcggcag cggaggagga agtgggatca
    11881 tatgcaattt acggatgatt ttcacccagc ttttggtcga ggtacagcat ctcgcaggtg
    11941 aatggaagtg gataaaggaa ttttcagcgg tcgaaaaaaa agcgtgagtg tggtgccatg
    12001 gggagccttt atgattgacc tttttttgac gatttttgga actgcagtgg tattaatttt
    12061 atcttttatt gcaccaattt ttcttttaac tggagaactt tttcttggta ttttgagctt
    12121 agggtattat cgacctcaac ttaaaaagga ggcccttaaa agcgaactgc catatagaga
    12181 gaggtcagtt tttgggttgg tctactaatt tggattggaa ttcttcttta tttaacatac
    12241 cctcaatagt gagtatggag aaaagaaaaa gtactcaaaa ttcgaggcct atctcttgaa
    12301 aaccttctgc ttcggggcgg gaggaacgca ctcagttcaa gtcgctggtt tcatggcctg
    12361 atgcaacata tcggtgatcc ataatttgaa gaatcgttgg gcggcttggt gatattcagt
    12421 cagttttctt gagatctctg tcatcgaaaa ttgtccattc acaaagaaat catgatcgat
    12481 gagatagccc atttcttgta taggttgcgc gatcggaacg attttatgtc gtacgcacac
    12541 caggtcatct ccaccattca gtcccatcac cacctcactg tgagtggctt tcatggctcc
    12601 ggccatctca ggccaggcga gatctcctgt ccatttgttc tgtagaagtt gattccaatc
    12661 tttgtccgta aggccaaggg cggacctccg aatgatattt ctgaaatgta accagatacg
    12721 ggagagatac ggtgggggat attttttctc taccgcgttc gtcgccaacc ggatatgggt
    12781 ctgaaaggcg tcccagcctt catatgggtg cattccggtg ttcagttttt cgtatttgac
    12841 ctgccgacac tcaggtctct ctcgtcttta tgaggctcca acgggcaggc gtgcttcctc
    12901 catgagcgtt aacatgccgg agagctgttt gcctgcttcc agcatgcact ctttgaggcc
    12961 ttcctcatcc agccctgtta atttccacga ttcaggagcc acgagatttt gccattgggc
    13021 atgtcccttg ggtgattgga acccttccgt gagggcgctg gccatattga ggagcgccgc
    13081 ttcaaatccg tgctcgctgg tcccggatgg attgttctgg tatcgaatag cgatcccgaa
    13141 attttttggc agttgccatt cctcaatgag tgtctgaccc acttcggtat aatcgaatcc
    13201 caacaactgt tgttccagga tatggacggg agagccatcc ttggccgact cccgtaacac
    13261 ctgttcagac ggttcgggaa gttgctcata gaggaggaga tgtccgatat ctctcaacag
    13321 gccctctaca aacatccgtt cactatccac cagaaaacat tttcttgcca attcccgtgc
    13381 gaccagtcca ctaaagaagc tgttggacca ataaacactc atactcatca cgtgttgatt
    13441 gaggcctgaa aaggttttgg taaccgagat ggcgagcgtc aaatcatgga gaggttgcat
    13501 cccgaggatt ccgacagccc gcgaaatggt ctcgactttt cgaggcatgg cgtaaaacgc
    13561 gctattcact aatttgagga cggcggcggt catgccggga tcaatgctga tcgccctgga
    13621 cagatcggcc atggtggact ctggattatc gatgaccttt ttgacttgta gatagacctc
    13681 cggaagggta aagagttttg tgcaattttt taccagttcc tcggctgtta gcatggtgat
    13741 cccttctggt gcagctctgg ggttcaatgg tctcaacgtc gaccctttct tatcattctc
    13801 ggatatttcc ctggggtact taaaatgaat tttttccgtt ccgggttggc tgttggacca
    13861 tggaactggg cggggttggt ccccaaacgg cttgttggtg gagaatgcgg gagagaggct
    13921 gtccctggtt tgtcggatca ggctgcagtc atactggttc cgcaacgagg aatgtgggag
    13981 gaatgatcag gcatgtttgg aaggatgaga ggttgagccc ggaggcattg ctgtaatttt
    14041 tttttggatg cggtttcttc cccatgtgat gatggcaaat gagaggccca gagcgatggg
    14101 cacgaagatt ccagaggcca actcatactt gtcgagccaa ccgagttcgt gcaaggcctt
    14161 aaagaggtag ttggctaggc cactcacgta ataggcaatc acgatcacgg agaggccttc
    14221 cacggtatgt tgcaagacga cctggctttt ggtggtctta tccaagctgg ccaggagatt
    14281 cacgttttga tcttgttgcg ccaaattttg ctcttgtaaa agtaattcca ccttggtgcg
    14341 caagacggca atggtgccct caaaatcctg ctctaaggcc tgaacacgtg agagcaattg
    14401 ctggtatcca tcggcgactc ccgtaatttt ccattgaaaa taatcgatga gcgtccgacc
    14461 gtaggacagc ggttgctctt ggagtgccag ggtattactg tgcacaattc gatcataggg
    14521 gaccgaagcc gaaaggcgat aacgcagcga cgcggtcaac cgtcccactt caagaaaatc
    14581 atgggttaac ccttccagcc attgttgaag tgtgctatgt gtggctcggt tcagttcttt
    14641 cgtgataagg gtccgttgat agagatgacg ctgttcgaag atttgaacct gatcgaccga
    14701 ccgggaaaag gctggatagg gcagtaaaat cagatgggtg taattttcaa gagtcgtcaa
    14761 aatcgtcatg agtctggcta ttttttgccg aagcaaacca ggatcaggag cccaaatgag
    14821 atatcgttcc cgatcatgtt cgtctggagt aaagctggtg gccacgcaga tgtcctcccc
    14881 cagaaccttg ctggcataca attgtggtcc ggggagaagg gttcctaatt cctcctggtc
    14941 gtaagtacgc gcagggatga ccaagagatc caacgcgttg acttggatgc ccaacggaga
    15001 aaagggaaac acatattggg aaaatcccaa cggtccgaat cccaatggct gggttgcgtt
    15061 agaggggata tgccaaacct gatagctgta atattcagtg tgagcctccc aaatgacaat
    15121 gagtttgtcc ccattcgtgc ctttctttac tccgaatcca accttgtctt ccatgacggc
    15181 gtgggtggga tcgatctcca agcaggccag aagctgttga aactcttttc gactttgagg
    15241 gcgttcaact gccgggttcg ccatacgata ggcggtgtgg tgcacatggg cgggaacatc
    15301 caaccagcct tccaaatatt tttcttgcgc cttatgcatt tggcgaagaa tgccttttcc
    15361 tggggatccg ggttttaatt tcatggacgt gtgaaaaatt gtgatgggaa tcgtgacgga
    15421 aaggattggc tgcgcatttc aacagagttc tacctcacct gagaggtctc aggcaagtgg
    15481 gggctaggcg tgaggggcgt tgagtgtttg aaggggattt aaatggcgtt caaagggctt
    15541 gagtccagga accgatcgtg gtcatgctga gttgagttgc gcatgaatcc agtcgcatag
    15601 gcgattttcg gcccaaaatg gatcctcatg gttttggtgc cgttgaaaaa atccgcaatg
    15661 tcccccatga gcaggcgcct caagctggat gaagggatta gcgtcgagag cagggtcggc
    15721 aaacatatgg taggggataa acgggtcgtc ctgggccgtg atgacgaggg tcgggatggt
    15781 gattgaagac agcgtgtttc gagcggcgct ttgttcgtaa tagtcttcgg cgtctcgata
    15841 ccctccgtct ggagcggtat agatttcgtc aaattcccac atggtccgta tatgagagag
    15901 atgagagaga tcccaccgac cgggaaagag gcgggctttt ttgcgtaatt ttcctgttaa
    15961 gcttttcagg aaatgacgat gataaagcca attggaggga cgttgcaggg cgcgtacgca
    16021 ggctgcgggc tggatgttcg gacagacggc tgcgactcca cgaagggagg ggagggtgga
    16081 tccatgctcg ccagctaatt tgagggcaag attgcctccc atggaatacc cgatgagcca
    16141 gactgcggtg cagccatcct cttcggtaat ttcttgaata atcctgccat aatcctggct
    16201 gagcccattg tgatagaggg tgggtgtgag atgttcggat ccgccacaat tgcgttgatt
    16261 gatgcgaatg caattccatc ccgcatccca acatttcctg gctagacctc gcatatagtg
    16321 ggattcggtg cacccttcga ggccgtgaac catcaggatg gtagggtgtt gtgaggggga
    16381 gggttgccaa tgacattttg tgagtatgcc cacctcggta gagacctgaa agatacggtc
    16441 ttgtgtgggt actccggatg ggaaacttcc acggggccac cagcgcggga gaagggtcat
    16501 gcggtgcggg ccatctagcc acgggtgtgg aacaaatggt tgggacggcc acaaacaatg
    16561 attaaccata agtgagcggg atgagcctta ggatgagggc ttttcagttg cttctcatga
    16621 tgggaatacc aaaactcctt tgcgtgagac aagttcggat aagggcattg tatagggagt
    16681 ggcccaatcg ggtgggtgtg atcacgttgc acggagagtg actatttgac
//
LOCUS       DATIAL010000547         6067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_97490, whole genome shotgun sequence.
ACCESSION   DATIAL010000547 DATIAL010000000
VERSION     DATIAL010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97490"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..1030
                     /locus_tag="VLA60_16700"
     CDS             374..1030
                     /locus_tag="VLA60_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11053.1"
                     /translation="MGTVEEVRGEQAKVDTGEGQPRYLPMSLREDKGLSALKKGDRVE
                     ITVNDQNLIVDVHLAGEESKHRTIQGVLAQPLVTGHEKAVIQTKEGQEESHKISSMAM
                     SKVASIPVGAEVIFLIDEMDQIVDVTFENQPGVEKTQHLGEKKSPLKGNFKKVSGILK
                     QPMKDNMVTVQLDGKKEQQYEVRPLIQEKFKKLSSGQEVILFVDDENKVTDVAFVEKD
                     "
     gene            complement(1447..2658)
                     /locus_tag="VLA60_16705"
     CDS             complement(1447..2658)
                     /locus_tag="VLA60_16705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HSF11054.1"
                     /translation="MSFWFIPLFSLEFSLFLLTALSLATWLYLSFGHGRFWYADQRLS
                     RDGPITFKTTQWPTVVVVVPARNEADVIERTLRSLLEQDYPGEFHVVMVDDQSEDRTG
                     DIARQLATDHPGGARLTVKVADDRPSGWLGKVWALHTGLQYAEKHWPDAAYRYLTDAD
                     IEHSRENLRELVSKAECEGLDLVSLMVRLHCQHAWEKLLIPAFVYFFQKLYPFPLIND
                     HNSPVGGAAGGCILVRNRALIQIGGIAVIRGEVIDDCALGAAIKRVGKIWVGLTDSEH
                     SIRPYVGLHDIWTMVKRTAYTQLRYSPWILVGTVMGLVLVYLLPPLVALTWPLHGSAL
                     GGGLALFAWLMMIYTFQPTLRLYALVPTYGFVLPIAAFLYLGMTVDSAWRYWLKIGGQ
                     WKGRTGIGCTN"
     gene            complement(2828..2953)
                     /locus_tag="VLA60_16710"
     CDS             complement(2828..2953)
                     /locus_tag="VLA60_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11055.1"
                     /translation="MITRIYMMLEHRLTALGYGMLKANNGIEDIVVLPIFTCGML"
     gene            complement(3092..4189)
                     /gene="modC"
                     /locus_tag="VLA60_16715"
     CDS             complement(3092..4189)
                     /gene="modC"
                     /locus_tag="VLA60_16715"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HSF11056.1"
                     /translation="MSELTARFEVVFPSFHLRVKASVPAEGITVVFGPSGCGKTTLLR
                     CLAGLERSPDGFLALGEQVWQDEAHNIFLPLSRRPVGYVFQEPRLFPHQSVRSNLLYG
                     WKRIPEPERRISLDQVVEILGLDSLLERRPTHLSGGEQQRVAIGRALLTSPRLLLMDE
                     PLSSLDVQRKREIMTFIQRLDKEFRIPIIYVSHALHEVVQLARTLILLKEGSVVAIGP
                     LAEVFAQVGSRRIIPDSHIGAIIDTRVAEHDLEFGLTTLKFSGGQLSVPHQHSSIGES
                     LRVQILARDVSLVASPPAFQTSVLNVLEATVLEIGPIESGQPFVDIKLDIGCPLLATI
                     TRKSLATLQLHPGQKVHAQIKAVALTHDSLE"
     gene            complement(4186..4869)
                     /gene="modB"
                     /locus_tag="VLA60_16720"
     CDS             complement(4186..4869)
                     /gene="modB"
                     /locus_tag="VLA60_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HSF11057.1"
                     /translation="MTLSEVDLGPLWLTLRLAGVTVIVLLIIGTPLAWWLAHTRSRAR
                     TAIEAIVAMPLVLPPTVLGFYLLVLLGPHGWIGSASQAITGSALSFTFTGLVIASALY
                     SLPFVVQPLHSAFESIGRKPLEAAWSLRASKLDTFLTIVSPMASRGYLTAIVLGFAHT
                     LGEFGVVLMVGGNIPGRTKVLSIAIYDHVEVLEYTQAHVLSAGLLVFSFLVLLMVYTV
                     NRRLPIHVS"
     gene            complement(4937..5701)
                     /gene="modA"
                     /locus_tag="VLA60_16725"
     CDS             complement(4937..5701)
                     /gene="modA"
                     /locus_tag="VLA60_16725"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HSF11058.1"
                     /translation="MMFLRVHTFGFMFFFYVCITAVAAEEVRVAVAANFLATLNEIIT
                     NFQADTSHTILVSSGSSGKLYAQIKNGAPFDVFFSADAERPQLLEEEGLAVKGSRFVY
                     SIGRLTLWSPDSNVIQGEAPHVLSDGRFEHLAIANPKTAPYGRAAEQTLKKMGLWESI
                     KDRIVQGENIGQAFQFVFSRNAQLGFVALSQVLDPKIDGSGSRWDVPATFHDALEQEA
                     VLLMPGQNHAGARAFLDFVKGDKSRAIIKQFGYELP"
     gene            complement(5782..>6067)
                     /locus_tag="VLA60_16730"
     CDS             complement(5782..>6067)
                     /locus_tag="VLA60_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11059.1"
                     /translation="VTMQIKQRFWYLAQGSAVNGGILLQDPTGGAGNYLGHDLELRVS
                     WVVSQNLDFDAGYDHWFKGSYFDRLPASANLPQGGNKDTDYFYLSMRIRL"
BASE COUNT         1675 a         1519 c         1492 g         1381 t
ORIGIN      
        1 aagacctcgg ccggtaaaca tgcctcgggg caataggctc tttcacctgg ctctccattc
       61 cattaccttt cttctgatcc tcacaaagag tcgtgaaggt ctacccaggg ttttccccag
      121 gtatcactga gaaagtattt ggatatatta aagttagtag tctgaatatc tgaaatttta
      181 ataaccaaag gagcgtttca tgtaccgcac aacatttatt tgccagacat caaggattcc
      241 atccaaaaaa agcttgtgtc tgtttggaac acattttttg attgtgtgcc ttacgttatc
      301 tggcgtggtg tttgctcatg aaggtactga cccgcaaagg agtgaatctt ctctggcagg
      361 ggaacgtgtc ataatgggaa ccgtagaaga agtcagaggg gaacaagcca aggtggacac
      421 gggggaaggg caaccgagat acctcccaat gagtctccgc gaggataaag ggctttcagc
      481 tctaaaaaaa ggggatcgcg ttgaaataac cgtcaacgat caaaacctca ttgtagatgt
      541 tcatctggca ggtgaagaat ccaaacatcg aaccatacag ggtgttttgg cccaaccact
      601 ggtgactgga cacgaaaagg ccgtcattca gacgaaggaa ggtcaagagg aatcgcataa
      661 gatcagttca atggctatga gcaaagtggc ctccatcccg gttggcgcgg aagtgatatt
      721 cctcattgat gaaatggatc agatcgttga cgttacattt gagaaccagc caggagtgga
      781 aaagactcaa catcttgggg aaaagaagtc tcctcttaaa gggaacttca aaaaagtttc
      841 aggaatcctg aaacaaccca tgaaagacaa tatggttact gttcaactgg atgggaaaaa
      901 ggaacaacag tatgaagtgc ggcctctgat tcaggaaaaa ttcaaaaaac tttcttcagg
      961 gcaagaagtg attctatttg tcgatgatga gaataaggtc accgatgtgg cctttgtaga
     1021 gaaagattaa aaattctctc cctgattggt tgaaggcaaa taattggaca aagcataacg
     1081 tttgttgaat tctatggtaa cgtgaaaaag gcatatgcac tccaaaagca ccggcaccaa
     1141 gagaaggaca aacacgggta gatccctttc caaccgatgc gtctaagtgt gctggaatta
     1201 tggtcagcag agtgttggag gcagcaggct cccgaccctg tgaaaagccg cctgaacgta
     1261 tagtgcggaa ttggccggga tccgcatttc tgccctttga gatattttca atcagggcgt
     1321 tgttgcgttt aggcggtttc gccacaccca ttatccatgg aaccttgtca gggtgaaaaa
     1381 tctggcgccc gccatcctgg gtgtccctgg gatggtaggc gactgtactg aatgaaaaac
     1441 caggactcag ttagtgcagc caattccagt ccgtcctttc cattgtcccc cgatctttaa
     1501 ccaatatcgc caagccgagt ctaccgtcat gcccaaatag agaaaggcgg ctatgggcaa
     1561 cacaaaacca taggtcggaa ccagtgcgta taaccgcaac gtgggttgaa acgtataaat
     1621 catcataagc caagcgaata gcgccagccc tccccccaga gcacttccgt gaaggggcca
     1681 ggtcaaggcg acaagcggtg ggaggagata gacaagcacc aggcccatga cggtacccac
     1741 aagtatccaa ggggaatatc tcagttgggt gtaggcggtc cgttttacca ttgtccagat
     1801 gtcatgaagc ccgacatagg ggcgaatgct gtgctctgaa tccgtaagac cgacccaaat
     1861 cttccctacc cgcttaattg ctgcacccag ggcacaatca tcaatcactt caccccgaat
     1921 gactgcgatc cctcctatct gtatcagcgc ccggttcctc acgaggatgc agcctccagc
     1981 cgcgcctccg acaggtgaat tgtgatcgtt aataagggga aagggataga gtttttgaaa
     2041 gaaatacaca aatgctggaa tcagcagctt ctcccaggcg tgttgacaat gtaatcggac
     2101 catgagcgat acaagatcaa gcccctcgca ttcggccttg gataccagct cacgcagatt
     2161 ttcccgactg tgttcaatgt cagcatccgt caaatatcga taggcggcat cgggccagtg
     2221 cttctcagca tactgcaatc cggtatggag agcccaaacc tttcccagcc atccagaggg
     2281 ccgatcatcg gcgactttta cagtaaggcg tgctccgcct gggtgatcgg tagcaagctg
     2341 acgtgcaatg tcgccagtac gatcctcact ttgatcatcc accatcacca cgtgaaattc
     2401 ccctgggtag tcctgctcta agagagagcg cagcgttcgt tcgatgacat ccgcctcatt
     2461 gcgggcggga acaacgacga cgacggtagg ccattgtgtg gttttaaaag taattggccc
     2521 atccctactg agtcgctggt cggcatacca aaatcggcca tgcccaaacg agagatagag
     2581 ccaggttgcc aaggaaaggg cggtaagcaa aaaaagggag aactcaagcg agaacaaagg
     2641 tatgaaccaa aaactcatga gtctcctcat agataaattc cagacgatca tcaatcatgt
     2701 gagttccctc aagaaccaga attttatttg tttcgcttaa gtatcgtcta cttaaaaagc
     2761 gttacaaaaa atcgatgtgg ggttccctca gcaaaaagag ttcattcatt cccgtggttt
     2821 caatatctca caacattccg catgtgaaaa tgggaaggac gacaatgtcc tcgatcccat
     2881 tgttggcttt caacatgcca taccccaagg ccgtcagccg atgctccaac atcatatata
     2941 tcctcgtgat catcacctac cacccaatgt tggcggcaat cgtccatcgg aacagtctgg
     3001 attccacttc ggcactctgg gttgaaacca cacccaagaa attaacgtat tgccattcgc
     3061 atcgagaaag aatttaggga gaatgggata tttattccag ggaatcgtgg gtaagggcca
     3121 ccgctttaat ttgagcatga actttctgcc ctggatggag ttggagggtc gctaatgatt
     3181 ttcgggtaat ggtggccagc aagggacaac caatatccaa tttgatatcg acaaagggtt
     3241 gcccagactc aataggtccg atttccagga ccgtcgcctc taacacattc agcacactgg
     3301 tttggaatgc aggtgggctt gccacaagac tgacatcccg ggcaagaatt tgtactcgca
     3361 atgattctcc tatggatgaa tgttgatggg gcactgacaa ctggcctccg gaaaatttga
     3421 gtgtggtaag tccgaattcc agatcatgtt ccgccacacg agtgtcaatg attgcaccaa
     3481 tgtgactatc cgggatgata cggcgactgc ctacttgagc aaacacttca gcaagaggcc
     3541 cgatagcgac cacgcttccc tccttcagca gaatgagagt cctggcgagt tgcaccactt
     3601 catgcagagc atgactcacg tagataatgg gaatcctgaa ttccttatcc agccgttgga
     3661 taaatgtcat gatttcccgc tttcgttgaa catcaagaga gctgagcggt tcatccatga
     3721 ggagcagccg cgggctggtt aataaagcac gcccaatggc cactcgttgt tgttctcctc
     3781 cggacaggtg ggttggccgg cgctccaaca atgagtctaa acccaggatc tcgaccacct
     3841 gatcaagtga aatacggcgc tccggttccg gtatccgctt ccatccgtat agtaagttcg
     3901 aacggacgct ctggtgagga aataaacggg gctcttgaaa cacatagcca accggacgtc
     3961 gggagagtgg aagaaatata ttgtgggctt catcctgcca aacctgttct cccagggcca
     4021 gaaatccatc cggtgaccgt tccaatccgg ctaaacagcg caagagcgtg gtcttgccgc
     4081 atccggaagg accgaatacg accgtgatcc cttcggcggg tacactcgcc ttcacccgca
     4141 ggtgaaatga gggaaagacc acctcgaacc tggctgtcaa ctcgctcatg agacgtggat
     4201 aggcaagcgg cggtttaccg tatagaccat aagaaggacc agaaatgaaa agacgagaag
     4261 tcctgccgac aggacatgag cctgggtata ttccaagact tccacatgat cgtagatggc
     4321 aatagagagt acctttgttc ttcctggaat attccctccc accatcaaga ccacaccaaa
     4381 ttcgccaagg gtatgagcga atccaaggac aatcgccgtg agatagccgc gagaggccat
     4441 cggggaaaca atggtcagaa atgtgtcgag ctttgaggct ctcagagacc aggccgcctc
     4501 taacggcttt cgcccgattg actcaaaagc gctatgcaag ggttgaacga cgaaaggaag
     4561 tgaatacaac gcggaagcaa tgacgagtcc ggtaaatgtg aacgaaagcg ccgatccggt
     4621 tatcgcctgc gaggcgctcc cgatccatcc atggggaccc aataggacca gtaaataaaa
     4681 tccgagcacg gtgggcggca atacgagagg catggcaaca atggcctcaa tggccgtccg
     4741 ggctcgggat cgggtatgtg caagccacca ggcaagcggc gttccaatga tcagaagcac
     4801 aatcaccgtc acccccgcca ggcgcaaggt cagccagaga ggtcctaagt ccacctcaga
     4861 tagtgtcatg gcttcctatg tagcattatc gccaatcgaa tgaaagtact tttctcatag
     4921 gtcgttatgt agttggttat ggcaactcat agccaaattg cttaataata gcccggcttt
     4981 tatctccttt gacaaaatct agaaatgctc tggcaccggc atgattttgt ccaggcatta
     5041 acaggaccgc ttcttgttca agtgcatcgt gaaaggtggc tggcacatcc caccggctgc
     5101 cgctcccgtc gattttggga tccaatactt gggaaagggc cacaaatccg agctgagcgt
     5161 tccttgaaaa cacaaattga aacgcttgtc cgatgttttc gccctgaacg atccgatctt
     5221 tgatggattc ccagagaccc atcttcttga gtgtttgttc ggcagccctg ccatagggcg
     5281 cggtcttcgg attggcaatg gccaggtgct cgaagcggcc gtctgataga acgtgtggcg
     5341 cctcgccttg aatcacgttt gaatccggac tccataaagt tagacgccct atagagtaaa
     5401 caaaccggct tcccttgact gccagacctt cctcttccaa caattgaggc ctctcggcat
     5461 ctgctgaaaa gaagacatcg aacggagcac cgtttttgat ttgtgcatac agttttccgc
     5521 tcgatccgga actgaccagt atcgtgtgac tcgtgtctgc ctgaaaattc gtgataattt
     5581 cgttaagagt tgccaaaaaa ttcgcggcaa cggccaccct gacttcctcg gcggcgacgg
     5641 ccgttataca tacataaaaa aagaacatga agccaaaagt atggactcgg agaaacatca
     5701 tgcacctccc aatacaataa gttccgacag atgactcatg atagagaacg ggctgcccct
     5761 gattaaggac tgagagcctg ttcacaatct gatgcgcatg gaaagataaa aataatccgt
     5821 atccttgttc ccgccttgag gcagattggc cgaagcagga aggcggtcga aatacgatcc
     5881 cttaaaccaa tgatcgtacc cggcatcaaa atccagattt tgacttacga cccaggacac
     5941 acgtaattcc agatcatgtc ctaaataatt tccggcccct cctgtaggat cttgtaaaag
     6001 aatccctcca ttcacggcac tcccttgggc taagtaccag aaccgctgct tgatttgcat
     6061 ggtcacg
//
LOCUS       DATIAL010000548        12522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_422133, whole genome shotgun sequence.
ACCESSION   DATIAL010000548 DATIAL010000000
VERSION     DATIAL010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12522
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422133"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VLA60_16735"
     CDS             <1..185
                     /locus_tag="VLA60_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11060.1"
                     /translation="PGLTLPITHGRRSEIAATLDHRTYFKIARFLPMHDPAVKTGTIV
                     EEGLKTFQAILPVITA"
     gene            complement(404..934)
                     /locus_tag="VLA60_16740"
     CDS             complement(404..934)
                     /locus_tag="VLA60_16740"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HSF11061.1"
                     /translation="MSQNGLSLILLAGFMLGVVFLLRWIWRRPLPAGVLPALNPESGE
                     SVVTARPIMSPEEATLYNLITLAARDHMLVLAKIPLLSVLSVVDKDEEARKAAMRTIQ
                     PVRCDVVLAHPGTLKVMTVITLNKDAPATAGREDRDRFVETLLKAAGIQLIILQTTQT
                     YSVDQLTGLLGLAEEE"
     gene            complement(989..2149)
                     /locus_tag="VLA60_16745"
     CDS             complement(989..2149)
                     /locus_tag="VLA60_16745"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HSF11062.1"
                     /translation="MIVVGMMSGTSADGVDAAVVNIRGTGHRLKSVLLKHVGRPYAPR
                     LRQRILQVCEQGTVGQICHLNVVLGEVFAKTALLAIKHSGISPQRVALIGSHGQTIHH
                     RPAAIYEPGIGQIRSTLQIGDPHVIAERTGITTVSDFRARDLAVGGEGAPLTPYVHAL
                     LFTQKHATRMVVNLGGIANVTLLPGGGNLAAVRAFDTGPCNMLLDGLVAMETKGIARF
                     DRGGRLAMKGQVDKGLLRWLLAHPYVSRRPPKSTGREMFGEDYIQRVWAQAKRRHLAI
                     SDLLATSCRFIAQVIHQAQKWTREVPDELVFGGGGVRNARLWSELSGVFDPIPVMRMD
                     ECGASSQAFEAQAFAVLAYQTVKGVCANLPKVTGARHSVILGTVTPGIHGLP"
     gene            complement(2228..2659)
                     /locus_tag="VLA60_16750"
     CDS             complement(2228..2659)
                     /locus_tag="VLA60_16750"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HSF11063.1"
                     /translation="MINFLDSHDVKAQQLLALFCQTDWACHRSLEDTERMLAHTDVAI
                     SAWDGSRLVGFGRVLTDFIYRASIWDVIVDRAYQDRDIGKGIIQRILTHPKLERVELF
                     WLCTRRYQGFYASLGFSDKEQTGMVWDRSKHIAPPTKPLNG"
     gene            2735..2914
                     /locus_tag="VLA60_16755"
     CDS             2735..2914
                     /locus_tag="VLA60_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11064.1"
                     /translation="MSMLVRKCPKCSKLFWKKEEEIQYADENTLKVVCNHCHQTLRIP
                     LITEGANAGAPKMGH"
     gene            3022..3747
                     /locus_tag="VLA60_16760"
     CDS             3022..3747
                     /locus_tag="VLA60_16760"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11065.1"
                     /translation="MRHEHWAGLDVCITGGVDRQGSGEGPLVVLLHGFGAPGDDLVAL
                     WRYLNVQDDVRFLFPAAPLQLGMGFGDARAWWMLDMERLTQARAQGQWEALSQEIPRG
                     LPPARTQMQDVLSLATEILSVPAPSLILGGFSQGAMLATDLVLHTDIPFAGLVLLSGT
                     LIAKQEWLTRLPNRQGLPVFQSHGTDDPILSFSMAQQLREHIQTAGLPVSWVEFRGGH
                     EIPIQVLKGLGTFLQSRLYPSSL"
     gene            4029..4277
                     /locus_tag="VLA60_16765"
     CDS             4029..4277
                     /locus_tag="VLA60_16765"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HSF11066.1"
                     /translation="MMTFEELSTQLQQALGDAEVSVLDRTGTMDHFTVKVISNAFEGK
                     NLLDRHRMIYQALDAPMKDGRIHALEIQAKTRNEAQGG"
     gene            4282..4614
                     /locus_tag="VLA60_16770"
     CDS             4282..4614
                     /locus_tag="VLA60_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HSF11067.1"
                     /translation="MSTPIEDEIKREIAQHKILIYGKGTKSAPQCGFTVETMEFFNKF
                     GYPYELINALPNPEKREALSKMTNWPTLPKVFINGQFYGDTDVLGPMEEKGELQPLLK
                     AAFPDQAT"
     gene            5130..5801
                     /locus_tag="VLA60_16775"
     CDS             5130..5801
                     /locus_tag="VLA60_16775"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF11068.1"
                     /translation="MTWTSAQGSHLTLPGSQDLSHGDESDSQPFIRQLEKINNYIPPH
                     AQPDHQNAASSKIEHSILPTNPLHPSPTEPKPIGSQQSHSPPSKKPIHIAVLLPNNDT
                     QDQTNTGWRSLLNGRPEEAIAAYQESLRIGPKSAEAYLGMGIALKSLGFIGHAKDAIQ
                     QALELNPHLSSALVHLGYLYADGHISPSDSKAAHRLFRQASQLGDPFASIALLDLQSR
                     SRPQS"
     gene            5940..6623
                     /locus_tag="VLA60_16780"
     CDS             5940..6623
                     /locus_tag="VLA60_16780"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSF11069.1"
                     /translation="MPKKLFRPFHHSQGFTLVEMIGVLAIIAILISLLLPKIFTLIAS
                     SKASSMAAAVRTYETAVAKYYGDIGTIWPLNAAGTPAQENGGNSATVTSLGARLTLDA
                     SDPLNTGANQWSRFRGPYLEKFNTNTPPGLGTTMYMPAQTAVALGTATTGTNVGWDLK
                     GDDGNSDLPTGARVAYLRVDGVSDTEFNEIDGVIDAGIGASLTERQLRGRVKYNPAND
                     RMYIYLTHQ"
     gene            6754..8442
                     /locus_tag="VLA60_16785"
     CDS             6754..8442
                     /locus_tag="VLA60_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HSF11070.1"
                     /translation="MPTTPIPPTAARPLLGTSLLQDGWITQEQLDLAVRETKRKGVML
                     GQTLIGLGFITEKVLAHYLAEGTQTGLVDLSTILIPPDIVNLIPYDIATQFQVLPLSK
                     QGNVLNLAMADPLNIVAVDTIESRIGLNVHTHTAPAQELIEAIERHYAHRESIKQLLD
                     ELMVKGISDLGEDGSRVAPMIRLCNQLITAAIQSRVTDIHVEPDEYYLRVRMRIDGIL
                     TQEILIPKPLQAPITARFKLMANLDLTEKRTPQDGRIPFTLGTRRIDLRVSTLPTNFG
                     ESIVLRILDKGALKLEFLALGFTPNDQSRIQSLIQRPHGIILVTGPTGSGKTTTLYTA
                     LRHVDAKEKSVFTLEDPIEYQMPLIRQTQVNPDIGMTFAAGLRALLRQDPDVILVGEI
                     RDQETADLAMRAALTGHLVFSTLHTNDALGAIPRLIDMGVEAFLLASALTAIIGQRLV
                     RCICPECKVERTDAATVLADLRVELPDSIPATLWTGKGCHACGQTGYKGRAGIYEIIQ
                     ISEELHGPIVHGPDMAGIRAIIRRDRMPTMFDDGLIKALQGITTLEEVLRVTGG"
     gene            8435..9646
                     /locus_tag="VLA60_16790"
     CDS             8435..9646
                     /locus_tag="VLA60_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSF11071.1"
                     /translation="MANFQYRAIDAHGKPIEGEMAAPDLFHLERQLQDMGLWLVRTLD
                     SKPKSLARTTQKRGKVKPRDLMEFSTHMFTLLEAGIPLTQALKSLSIETPNLHLRTTL
                     QAIFQQVEAGNTLHDAMKQHPRIFPPQITSLVHAGEDSGTLTETFKELERYLDWLDQI
                     RGDVRQATIYPSIVMVAVIGLLVLLFTFVIPRFVPILEGLHVPLPTITILIITLSELF
                     VGSWWLWSSFLLLGPMVWTLGRKNLPGFAHWRDRMVLQLPVLGELIRMLTISKFVQNF
                     AVLYRAGIPVLQCLQLCQGLVGNSVMERALQDSQRAVSEGSTINESLSRHPVFPPMVI
                     QMISVGEATGTLPHTLMNVANYYNREIPRRIKKVFGILEPLITLSLIAIVEVVALSLF
                     MPMMSLMGGIH"
     gene            9643..10446
                     /locus_tag="VLA60_16795"
     CDS             9643..10446
                     /locus_tag="VLA60_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11072.1"
                     /translation="MTGYLGQPDVALPSPPRQQNASDPEAGVTLIVFIGLLAILSIGL
                     ALVSPSMVRVWDRTSEDRETRDLQFIANGVISYLRQNHAFPPSLLILSPDFVPIPPAQ
                     MTRNARGFPRYYAVHPSITGFQNSTGLTGGELANARFLLISNLAQNAAPAITTPASFE
                     TWWNMNDSTTPPLIIHRETVSHLFYMVNLIPMGNGGSFSLDHQPTHSNGALLPTHGRV
                     QLLGTEIGLDEDNSYNTPDIAFSLTTNTGYWFDPLCVATKRWNPLSPPC"
     gene            10727..11647
                     /locus_tag="VLA60_16800"
     CDS             10727..11647
                     /locus_tag="VLA60_16800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSF11073.1"
                     /translation="MTRRNDFPTQAIGLQTASIAIETGWRSGTGLRFTPCGNRNTVVR
                     IGRKFFGLFAASRCLSTNGFTLVEMIGVLAIIAILASLIAPNVINQLAAVRRDAEDEQ
                     LATIARGIELYLRQTRSFPATLTELSPDYVAAALGQLTTNPNGFLRYYFVQPNISGFT
                     NATGLSTTALADARFMVITHLSQNVNPSITTDADFETWWSTDETGIPDLKIHRGHVGH
                     LFHLLSLSANGAGGSYAVDGSPTDSGGGTLVPHSRYHFSGTTISLDEANTFSVPETQF
                     TLTSEAGFQFDPDCATGSKWRINSSGCYSP"
     gene            11652..>12522
                     /locus_tag="VLA60_16805"
     CDS             11652..>12522
                     /locus_tag="VLA60_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11074.1"
                     /translation="MIDTPQSPTLFADAQTPRLEIPLNLQKTLAKKKQTILSVSLSNG
                     RLKALSIVKNTIGRSWERPGRHVSLDTLREALEEAIHHTQFPGTHLAMLVDHPRLASL
                     TIQIPPMPPTDLLPFLERKVQQEKPWEGPAAWRYQIGLESRGKLHIHLDIWPQDYIDK
                     IVHICQELGLSLRQLAPLSTLAESQLSTLPVEPGKGSILITMIEGKIMIVAGNDDGTR
                     IWTRHLFPAQDWVPLGERVGTEANRTIMFITQQTNLKFPCIWFWDDEERLTAGEIQPH
                     VNTPLVPYPIKPDW"
BASE COUNT         3163 a         3606 c         2850 g         2903 t
ORIGIN      
        1 gacctgggtt gacacttcca attacacacg gtcgacgatc agaaatcgct gcaacactcg
       61 accatcgtac atactttaaa attgccaggt ttcttccaat gcatgatccg gcagtcaaaa
      121 caggcaccat cgttgaggaa ggtctgaaaa cctttcaggc gatcctaccg gtcattacgg
      181 cctaggatcc tcgcggactt cggatggcag caaaagtgcc tgagcggtcc atatttcgcc
      241 gcattcttgg cccatggtcc cactgtgggc cggaaaatcg ccaaaattcg cccttgctta
      301 gccacatttt cgttatcgat tcacaaagtc tgacagactc ctagacaccc cacattcctc
      361 gcgtacaagg tccttaccca aaaatatcct ttggttcccg gacctattct tcctcagcca
      421 gaccaagtaa tccggtgagc tgatccaccg aataggtctg tgtggtttga agaatgatca
      481 attgaatacc cgccgccttc aacagggtct ccacaaatcg atctcggtct tcacgtcctg
      541 ctgtagcagg agcatccttg ttcaaggtaa tcacggtcat gactttaagt gtcccgggat
      601 gggcaagaac gacatcacag cgaacaggct gaatcgttcg catggctgcc tttcgagctt
      661 cttcatcttt atcgacaacg gacaggacac tgagaagcgg gattttggcc aaaaccagca
      721 tatgatcacg agccgctaag gtaatcagat tgtagagagt cgcttcttcc ggcgacatga
      781 tgggtcgagc ggtcaccaca ctctcgcccg attcaggatt caaagcaggg agaacccctg
      841 ctggaagggg acggcgccag atccatcgca acaaaaacac gactccgagc atgaacccag
      901 ccagaagaat tagtgagagc ccgttctgag acatgaatct gtccgtcatt tcaccagtgg
      961 gaaaaagccc gtaacaataa ggaacattct agggaagtcc gtgaataccg ggagtgaccg
     1021 tccccaaaat taccgaatgg cgagcacccg tgaccttagg cagattggca cagacaccct
     1081 tcacggtttg atacgccagc accgcaaagg cctgcgcctc aaaggcctga ctggatgccc
     1141 cacattcatc catgcgcatc acgggaatgg gatcgaacac accggacaat tctgaccaga
     1201 gccgggcatt tcgcacccct ccaccgccaa acaccaactc atcagggact tccctcgtcc
     1261 acttttgcgc ttggtgaatt acctgagcaa taaaccggca actcgtggct aagaggtcag
     1321 aaatagccaa atggcgacgc ttcgcttgag cccacacacg ctggatatag tcctctccaa
     1381 acatctcacg cccagtggat ttgggaggcc ttcgagacac atagggatgt gccagcagcc
     1441 aacgaagcag gcccttatcc acctgtccct tcatggcaag acgaccaccc cggtcaaatc
     1501 tggctatacc ctttgtttcc atagccacca atccatccaa caacatattg cagggccctg
     1561 tatcaaacgc ccgaaccgcc gccaggtttc ccccgccggg caatagggtc acattcgcaa
     1621 tccctcctag attgaccacc attcgtgtgg catgtttctg agtaaaaaga agggcatgga
     1681 cataaggagt caatggagct ccctctccac cgaccgccag atctctggct cgaaaatccg
     1741 atacagtcgt gattccggta cgttccgcaa ttacatgggg atctccaatt tgcagtgtcg
     1801 aacgaatctg cccaatacca ggctcataga tcgcggcagg ccgatgatga atcgtttgac
     1861 catgcgagcc gatcaacgcc actcgttgag gagaaatgcc ggaatgcttg atggctaaca
     1921 acgcggtttt agcaaatact tcgcccagga ccacattgag gtgacagatc tgccctacgg
     1981 ttccctgctc acaaacttgt aaaattcgtt gacgaagcct gggagcatac ggacgaccaa
     2041 catgtttgag cagtacactc ttgagacgat gtccggttcc tcgaatattc acaactgcgg
     2101 catccacccc atctgccgat gtccctgaca tcatccccac aacaatcatc gattccctcg
     2161 attcattggc ctgcgtgctt cattggaatg acactcgata tgttgcaacc atcgccatgg
     2221 cccatctcta cccattcaat ggtttcgtgg gaggagctat atgtttgctc cgatcccaga
     2281 ccatgcccgt ctgttcttta tcagaaaatc ccaaagaagc gtaaaacccc tgatagcgcc
     2341 tggtgcaaag ccaaaacaac tccacccgtt ccaacttggg atgcgttaaa attcgctgga
     2401 taatcccttt accaatatcc cgatcctgat aagcccgatc cacgatcaca tcccaaatcg
     2461 aagcccgata gatgaaatcc gtcaacaccc tcccaaaccc gacgagtctt gagccatccc
     2521 aggcggaaat ggccacatcc gtatgagcga gcatgcgttc agtgtcctcc agtgaacgat
     2581 gacaagccca atccgtttgg cagaacaatg ccaaaagctg ttgcgccttg acgtcatggg
     2641 aatccaaaaa attgatcatg cgttggcaga ttgtggcaag atgtattaca ctaagtctac
     2701 tagaaatacg tgacaaaaaa ctggagtcgt gccgatgtcg atgttagtca gaaaatgtcc
     2761 aaaatgcagt aagttatttt ggaaaaaaga agaagagatc caatatgccg acgagaatac
     2821 cctgaaagtc gtctgcaatc actgtcacca gaccttgcga attccgctca tcacagaagg
     2881 cgccaatgcc ggtgccccga aaatgggaca ttaggatttt taaaccttcc ccttccagca
     2941 tcagagtcgc gcgtttatca aaatcatggc caattgaaaa tattcccctt ctctgttctt
     3001 catcattaga cttttgactg tatgcgacac gaacattggg ccggattaga tgtctgtatt
     3061 accggtggcg tggatcgcca agggagcggc gagggaccac tcgtggttct cttgcatgga
     3121 ttcggagctc caggagatga tttagtcgcc ttatggcggt atctcaatgt ccaggatgac
     3181 gtccgtttcc tctttcctgc cgctccactc caattgggca tgggctttgg ggacgcccga
     3241 gcttggtgga tgctggacat ggaacgcctc acacaagcca gggcccaagg acagtgggag
     3301 gcgttgtcac aagaaatccc ccgtggcttg cctccggccc gcacccaaat gcaggatgtc
     3361 ctctctctcg ccacagaaat cttgtccgta ccagcaccat cgttgatcct tggagggttt
     3421 tctcagggag ccatgctggc gaccgatctg gtccttcata ccgatattcc cttcgcagga
     3481 ttggtactac tatcgggcac cctcattgcc aagcaagaat ggctcactcg cctacccaat
     3541 cgccaaggtc tcccggtatt tcaaagccat ggcaccgacg atcctatttt atcgttttcc
     3601 atggcgcagc aactacgtga gcatatccaa acggctggat tacccgtcag ttgggtagaa
     3661 ttccgtgggg gccatgaaat tcccattcaa gtattgaagg gcctcggaac ctttctacaa
     3721 tcacgtcttt atccttcttc gctctagaaa aataatgcgg taaaagtttt tggagtcagg
     3781 agacaaaagg aggactccct cctcacctat ttccttgcct atctccattt tgattcctga
     3841 cgcctcactc cttatttcac ttcggatcac aggctctctt cataccgcac ggacaggact
     3901 aaacattgct ccccctagga accattcatt ctgagaagat tccagacccg cattgcgcag
     3961 aaaattctct ctccggtata gtggagtctg aatattcaat catttttcac caggattaaa
     4021 ttttttctat gatgacattt gaagagctga gcactcaact tcagcaagcc ctaggcgatg
     4081 ccgaagtttc ggtccttgat cgaaccggta ccatggacca ttttacggtc aaagtcattt
     4141 ccaatgcctt tgaaggaaag aatttattgg accgtcatcg aatgatttac caagccctcg
     4201 acgcgcccat gaaagacgga cgcattcatg ccctagaaat tcaggccaaa acccgcaatg
     4261 aagcccaagg aggataatct tatgagtacg cccattgaag acgaaattaa gagagaaatc
     4321 gcccaacaca aaattctcat ttacggaaag ggcaccaagt ctgccccaca gtgcggattt
     4381 accgtggaga ccatggagtt ttttaacaaa ttcggctatc cctatgaatt aatcaacgcc
     4441 ttgccaaatc ctgaaaaacg ggaggctctt tctaaaatga ccaactggcc gactttgcca
     4501 aaagttttca ttaatggcca gttttatggg gatacggatg tcttgggccc aatggaagaa
     4561 aaaggggaat tgcagccact tttaaaagca gcgtttcccg atcaagccac ctaacaagca
     4621 aaccaaacgt ttcctggctc aacaaggccc acctgagtca cacaggtggg ctttgttatt
     4681 tgcctcagac cctaccctag tcggaacact cctccagcaa gacccgacct ttcctcttgt
     4741 tcatcaccca aattctcaac acacctcaga cacttctttc attgatgcct ctaaggatcc
     4801 tgtgggcgtt catggcctca gggtttgaat cgtggcaggg ctatcatgcc ttccctaagc
     4861 cctccgatta tccctcaacc ccatgtattc ttttgttggc agttcctagc ttattgaggt
     4921 gggtttttgg caattcggtt aacacttcac gcgtaatccc ttaagcatgt tgcctcttgt
     4981 ccgataggtt caacctggaa ccactgaggc aatgagccat catgagacat cccatcttaa
     5041 taccaaggga aaaagccaaa agatcatcta aagccctagg gtcatcttca gtcacgttca
     5101 tcctcgccat aatcattggt ctagagataa tgacctggac ttccgcccag ggaagtcatt
     5161 taacgctccc cggctcccag gacttgagtc atggagatga atctgactca cagcctttca
     5221 tccggcaact agaaaagatc aacaactata tccctcctca cgctcagcct gatcaccaaa
     5281 atgccgcctc atcaaagatt gagcactcca ttcttcctac caatcccctt cacccgtcgc
     5341 ccaccgaacc caaacctata ggatctcagc aatcccattc tcctccatca aaaaaaccca
     5401 tacacatcgc agtcctgctt cccaacaatg atacacaaga ccagacgaat acgggatggc
     5461 ggtcattgtt aaatggtcgc ccggaagagg cgatagcggc ttatcaagaa tccctcagga
     5521 tcggccccaa atcagccgag gcctatctcg gcatgggcat cgcgctgaaa agcctgggct
     5581 ttattggcca tgccaaagat gccattcaac aggcgcttga actcaaccct catctttctt
     5641 cagcactggt tcatctggga tatctatatg ctgatggcca catcagcccc tcagattcca
     5701 aagccgctca ccgacttttc cgtcaagcct ctcaactcgg ggatccattt gccagtatcg
     5761 cactcctaga cctgcaatcc cgctctcggc cgcaatccta atttccggac aaggggtggg
     5821 cttaagaatg ccatcccgga tgccgatgga ttttttaggg gcgtgtcatc cataccattt
     5881 tccatattca gccttaaact cggccacacg atgcgccctt cgtaaaggag aactgacaga
     5941 tgccaaaaaa actattccgg ccgtttcatc attcccaggg gtttaccctg gtggaaatga
     6001 tcggggtatt ggccatcatc gccatactga tttccttgtt actgccaaag attttcactc
     6061 tgattgcctc gtctaaagcc agttccatgg ctgccgctgt gagaacgtat gagaccgccg
     6121 tcgcaaagta ttatggagac atcggaacca tctggcctct caatgcggct ggaactccgg
     6181 cacaagagaa cggaggaaat agcgccaccg ttacctctct tggagctcgt ttaacgctcg
     6241 acgcctccga tccgttgaat accggagcga atcaatggag ccgattccga gggccctatc
     6301 tggaaaaatt taataccaat acgcctccgg gactgggcac aaccatgtac atgccggcgc
     6361 aaacggcagt tgcactcggg acagcgacaa cggggacgaa tgtaggatgg gatctgaagg
     6421 gtgatgatgg caacagcgat ctccccactg gagcccgagt ggcctatctc agagtcgacg
     6481 gagtttccga cacggaattc aatgaaattg atggagtcat cgatgccggt atcggtgcaa
     6541 gcttaaccga acgccaatta cgcggacgcg tgaaatacaa cccggccaat gatcgcatgt
     6601 atatttatct tacacatcaa taaatcacat cctcgttcat tgagggatga ttgagaaaat
     6661 cccaacaatg gctgtgctcc ctcctcctat gcgttcctca caggcgagca cccatttgct
     6721 gtcgcccata aacttctcct agcagtccgg cccatgccaa ccacacccat acctcccaca
     6781 gccgcacgtc ctctcctcgg aacgtctctc ctccaagacg gctggatcac tcaagaacaa
     6841 ttggacttgg cggtccggga gacgaagaga aaaggggtaa tgctcggcca aacgctcatt
     6901 ggcctgggat tcattaccga aaaagtcctg gcccattacc tggctgaggg cacccaaacc
     6961 ggcttagtcg acctctccac gatcctcatc ccaccggaca ttgttaatct catcccctac
     7021 gacatcgcca cccagtttca agttctccct cttagcaagc aggggaatgt gttgaacctg
     7081 gccatggcag atccattgaa tattgtcgcg gtggatacca ttgaaagtcg catcgggctt
     7141 aacgtccaca cccacacggc ccctgcccaa gagttaattg aagcgatcga gcggcactat
     7201 gcgcaccggg aatccattaa gcaactgctt gatgaattaa tggtcaaagg gatctccgat
     7261 ctcggggaag acggaagccg ggtggcgccg atgattcgct tgtgcaatca actcattacc
     7321 gccgccattc aatcacgggt gacagatatc catgtcgagc cggatgagta ctatttacgc
     7381 gtccgaatgc gcattgacgg cattctcaca caagaaattc tcattccaaa accgcttcaa
     7441 gcgcccatta ccgcccggtt taaactcatg gccaatcttg atctcacaga aaaacgcact
     7501 ccccaggacg gccggatccc ctttacctta ggcactcgcc gaattgatct tcgtgtgtcg
     7561 acccttccaa cgaatttcgg agaaagcatt gtcctccgaa ttctggataa aggggcattg
     7621 aagttagaat tcctggcgtt ggggtttaca cctaacgacc aatcgcgcat ccaatccctg
     7681 atccaacgtc cccatggcat tatcctcgtg acgggtccca caggaagtgg aaagaccaca
     7741 acgttatata cggccctccg tcatgtcgat gcgaaagaaa aaagtgtgtt tacactggaa
     7801 gacccgatcg aataccaaat gcctctcatt cgccaaacgc aagtcaatcc agacatcggc
     7861 atgacctttg cggccggact tcgtgcccta cttcggcagg atcctgatgt cattcttgtg
     7921 ggtgaaatcc gcgatcaaga aacggccgat ctggccatgc gcgcggcctt aaccggacat
     7981 ttggtttttt ccacacttca caccaacgat gcgctaggag ccattccccg tttaattgac
     8041 atgggcgtgg aagcattcct cctcgcctca gccctgaccg ccattatcgg gcaacgactc
     8101 gttcggtgta tttgtcccga atgcaaggtt gaacgaacgg atgccgccac tgtccttgcg
     8161 gatcttcgag tggaattgcc ggacagcatt cccgcgactc tctggacggg aaaaggatgc
     8221 cacgcatgcg gccagacagg ctataaaggc cgggcaggta tttacgaaat aatccagatt
     8281 tctgaggaac ttcatgggcc tatcgtgcac ggcccggata tggccggtat tcgggcaatt
     8341 atccggcggg atcggatgcc aacgatgttt gatgacggac tcataaaagc cctgcaggga
     8401 atcaccactc tcgaagaagt gttgagggtg accggtggct aattttcagt accgcgccat
     8461 cgacgcccat gggaagccga ttgagggaga aatggcggcg ccggatcttt tccacctgga
     8521 acgccagctc caggacatgg gactctggct cgtgcgcaca ttggactcca aaccgaagtc
     8581 cctcgcacga accacccaaa aaagagggaa agtcaaaccc cgcgatctca tggaattctc
     8641 cacacacatg tttaccctcc tcgaggccgg cattcccctt acccaggctt taaaaagttt
     8701 gtcgatcgaa acacccaacc ttcatttgcg tacaaccttg caggcaatct ttcaacaagt
     8761 cgaggccgga aacaccttgc atgatgccat gaagcaacac cccaggattt ttcctcctca
     8821 aatcaccagt ctggtgcatg ccggagaaga cagcggcaca ttgaccgaga ccttcaaaga
     8881 attagaacga tatctggatt ggctcgatca gatccgtgga gatgtccgtc aggccaccat
     8941 ttatccgagc atcgtcatgg tagcggtgat cggattactg gtgttgctct tcacgttcgt
     9001 cattccccga tttgtcccta tcctggaagg tctgcatgtg cctctcccta ctatcaccat
     9061 tctcattatc accctcagtg aactcttcgt cgggagctgg tggctctgga gttcgttcct
     9121 cctgctcggc ccaatggtgt ggaccctggg gcgaaaaaac ctgccgggat tcgctcactg
     9181 gcgggaccgc atggtcttac aactcccagt tctgggggaa ctcatccgga tgctcacgat
     9241 atccaaattt gttcaaaatt ttgccgtctt gtatcgagcc ggcatccctg ttctgcaatg
     9301 cctgcaactg tgccagggac tggtgggcaa ttctgtcatg gaacgggcgc ttcaggactc
     9361 gcagcgcgcc gtgtccgagg gctccaccat caatgaatca ttaagccgtc atccggtctt
     9421 tccgccaatg gtgattcaaa tgatctcggt cggggaagcc accgggacat taccccacac
     9481 actcatgaat gtggcgaatt attacaatcg ggaaattccc cgcagaatca aaaaagtttt
     9541 tggcattctc gagcccctga ttactctgag cttaatcgcg atcgtggaag tcgtcgcgct
     9601 atcactcttt atgcccatga tgagtttaat gggtggaatc cattgacagg ctacctgggc
     9661 cagcctgacg tcgccctacc ttccccgccc cgccaacaga atgcctctga cccggaagcc
     9721 ggagtgaccc tcattgtctt tatcggcttg ttggcgattc tatctatagg gctcgctctt
     9781 gtgtcgccct ccatggtcag agtctgggat cgaacgagtg aagaccgtga aacccgtgac
     9841 cttcagttca tcgccaacgg tgtcatttcg tatctacgac agaaccacgc gttcccccct
     9901 tcgttactca tcctttcccc tgactttgtg ccaatacctc cggcccaaat gacgcgaaac
     9961 gcacggggat ttccccgcta ttatgcggtg catccctcca taaccggatt tcaaaactcg
    10021 accggactga ctggcggtga actcgccaat gcccggtttc ttctcatatc caatctcgca
    10081 caaaatgcgg ccccggccat caccacaccc gcctcctttg aaacctggtg gaacatgaat
    10141 gacagcacga ctcctccact cattattcat cgtgagacgg tcagccatct tttttatatg
    10201 gtcaatctta tccccatggg aaatggggga agtttttccc ttgaccacca accaactcat
    10261 tccaacggcg cgcttcttcc aacacatggc cgcgttcaac ttctgggcac tgaaatcggt
    10321 ttggacgagg acaatagcta caacacccct gatattgcat tctctctcac caccaatacc
    10381 ggctattggt tcgatccctt gtgcgtcgcc accaaacgat ggaatcctct gtctccgccc
    10441 tgttaggcgg aaacattctc gagactttct tactccctga cccggcaaaa aatgactgct
    10501 ttcctatctc cctaacctca cctccggctg atcctcccgc ccccttgtgg tccttgcaaa
    10561 cccgccatga tcatttgaaa agtttaagaa aattaagatt ggcattttaa acaccgaaag
    10621 aagggttggt ggactacgag gcgggatatg cccgaggaga gaagagtacg tacacgcacc
    10681 ccgggacaaa agccccgctg agaaggagca agacgggatc ggactcatga caagacgcaa
    10741 tgactttccc actcaggcca tagggctcca aacggcctcg atcgcaatcg agactggctg
    10801 gcgttccggc accggcctcc ggttcactcc gtgcggtaac cggaacaccg ttgtaagaat
    10861 aggccggaaa tttttcgggt tatttgcggc ctcacgctgt ctatctacca atggatttac
    10921 gcttgtggaa atgattggcg tcctggcaat tatcgccatt cttgccagcc tcattgcgcc
    10981 aaacgtgatc aatcaactgg ccgccgtgag gcgggatgcg gaggatgaac aattggcaac
    11041 aattgcccga ggtattgaac tctaccttcg gcagacccgt tcatttccgg caacactcac
    11101 cgagctgagt ccggattatg tcgcggccgc tctaggacaa ctcaccacga accctaatgg
    11161 atttctccgc tattacttcg tgcaacccaa cattagtgga ttcaccaacg ccacaggtct
    11221 ctccacgaca gccttggcgg acgcacgatt tatggtcatt acccatctgt cccaaaatgt
    11281 gaatccctcc attaccacgg atgccgactt tgaaacctgg tggagcaccg atgaaacagg
    11341 aataccggat cttaaaattc atcgcgggca cgttggccat ttattccatc tcctcagcct
    11401 ctctgccaat ggggccggag gcagttacgc ggtcgatggg agcccgactg attccggtgg
    11461 cggcacgctt gtgccgcata gtcgctatca cttctcggga accacgatta gtttggacga
    11521 agcgaatacc tttagtgttc cagaaacaca attcaccctg accagcgaag ccggatttca
    11581 attcgacccc gactgcgcga caggatccaa atggcgaatc aacagtagcg gttgctatag
    11641 cccctaatac tatgatcgac accccgcaat cacccacact atttgccgac gcccagacac
    11701 ctagactgga gatcccactg aacctccaga agaccctggc gaagaaaaaa cagacgattt
    11761 taagcgtcag tctttcaaat ggtcgattga aagctttatc gattgtgaaa aataccattg
    11821 gacggagttg ggaacgtcca ggccggcacg tatccctcga cacacttcgc gaagcattgg
    11881 aagaagcgat tcaccacacc caatttcccg gcacgcacct cgccatgctc gttgaccatc
    11941 cgcggcttgc ctccctgacc attcaaatcc ctccgatgcc ccccaccgac ctccttccct
    12001 ttcttgaacg aaaggtccaa caggaaaaac cgtgggaggg accagcggct tggcgatatc
    12061 agattgggct ggaatcgcga ggaaaattac atattcacct tgatatatgg ccacaagact
    12121 atatcgataa gatcgtgcac atctgccaag agttgggctt atctcttcgt cagttggctc
    12181 ctctgtctac tctggctgag agtcaactca gcacgttgcc cgtcgaaccg ggaaaagggt
    12241 cgatcctgat aaccatgatc gaaggcaaaa tcatgattgt cgcaggaaat gatgatggaa
    12301 cccgcatctg gactcgacat ctgtttcccg ctcaagactg ggttccactt ggggagcggg
    12361 tcggaaccga ggccaatcga acgataatgt ttatcacaca acaaacaaat ttaaagtttc
    12421 cctgcatctg gttttgggat gatgaagaac gattaacggc gggtgaaatt cagccacacg
    12481 taaatactcc cctcgtacca taccccatca agccggactg ga
//
LOCUS       DATIAL010000549         3502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1280431, whole genome shotgun sequence.
ACCESSION   DATIAL010000549 DATIAL010000000
VERSION     DATIAL010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280431"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..379)
                     /locus_tag="VLA60_16810"
     CDS             complement(41..379)
                     /locus_tag="VLA60_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 domain-containing protein"
                     /protein_id="HSF11075.1"
                     /translation="MPEWVRYGLYFFLGGLLVSVSTYLGSHGKGFLAALASTLPVISG
                     LTFILVYVNAGSAPTVSFAKNLIWLSPPWFVYVGTMIAVVPKLGFWPAYGLAFGLYLA
                     GVGLTRLFVH"
     gene            complement(404..1414)
                     /locus_tag="VLA60_16815"
     CDS             complement(404..1414)
                     /locus_tag="VLA60_16815"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11076.1"
                     /translation="MTPSFLPRLVNHPFGDPGLYVEVRWSRRALLFDLGENLSLNPTQ
                     LLRAQDIFVSHTHMDHFIGFDRLIRIALGRGKHLRLYGPPGLIENINGKLRGYTWNLV
                     DGYPLTLEVKEFHPSHIQSAHFHAKDAFMCQLNAPIPGDHSHIFTVLQEPALLVKAVA
                     LNHRIPSLAFALEEPFHINVNKARLHEAGLPVGSWLKDVKQLIWDGAPDSHTFSATLY
                     VEHRKEERIFNVGTVKHRFTTITRGQKIAYVVDCRYDEDNEQKIIQLCRGADTLFCEA
                     PFLDADREKAANRYHLTANQAGILGRKAEVQELNVFHFSPRYTGQEDRLQQEAMQAFY
                     GY"
     gene            complement(1411..2106)
                     /locus_tag="VLA60_16820"
     CDS             complement(1411..2106)
                     /locus_tag="VLA60_16820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11077.1"
                     /translation="MTPHKERAAGLAFIPVFATIGFYALPLNWQTPFAIQFTPQLIAY
                     LALSVWACRNVGRLVKLGLSFDNIQPGIFWGTITGISLGTMNTGIILYALPALEMDIS
                     FLSKTPHAQIPFWIMAPWFIIVIAMAVELNFRGFVLGRLLVFFDDVLQTAHPSVMRNL
                     MQVILPLGISALTFSFDPFLVTTFRHLHWIAVWDGLIWGWMWMKMHNLYAIMTAHAVE
                     VVMIYLIIRGILE"
     gene            2771..>3502
                     /locus_tag="VLA60_16825"
     CDS             2771..>3502
                     /locus_tag="VLA60_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11078.1"
                     /translation="MKQERLSLGRCIYRRIVGKKFGEIRDRIAGPGLMVMMVLSLFGA
                     VGSEGYADDPPQELPKPFHKTTPPLFQEWNFDQSASNGFPEGFTQALGGIQGEGEWSV
                     KEDPSAPSRPRLVEQRLHCEEDWCYQLLIAEGVVAEYVDLSVRMKLNLGTPSGKAGLA
                     YGIQDDKNFYAVVVEPKTNEVVAYVVKNGQPTELARESLIPQPSDWHFLRIQRNTIIS
                     KEFTEIFFDHHLMIDVYDQSFKKGKL"
BASE COUNT          980 a          783 c          879 g          860 t
ORIGIN      
        1 aaacagaatt tggtagcgca aaatttatca tctgactctg ttaatgaacg aacaatctgg
       61 tcaagccgac tcctgccaag tataatccga aagccaatcc gtaggccggc caaaagccta
      121 gttttgggac gacggcaatc atcgttccca catagacaaa ccatgggggt gagagccaga
      181 tcaaattttt ggcaaatgag accgttggag ccgatcccgc attgacatag actaagataa
      241 atgtcaatcc gctaataacc ggcaatgtac tcgccaaggc ggccagaaac cctttgccat
      301 ggctcccaag gtaggtagag acgctgacta acaatccccc aaggaaaaaa tacaatccat
      361 atctgaccca ttccggcatt gtccctccac gagttgtgtc tagttaatat ccataaaagg
      421 cctgcattgc ctcttgttga agccggtctt cctgtccggt atatcgagga gaaaaatgaa
      481 aaacgttcaa ttcttgcact tcggcttttc gtccaaggat tccggcttga ttggctgtga
      541 ggtggtagcg gttggcggcc ttttcccggt ccgcatccaa aaatggtgcc tcgcaaaata
      601 gggtatccgc cccacggcat aactgaataa ttttctgctc attgtcttca tcataccggc
      661 aatcgacgac ataggcgatc ttttggcctc gggtaattgt cgtaaatcgg tgctttactg
      721 tgcccacatt aaatatccgt tcttctttcc gatgttcaac atataacgtg gcagaaaagg
      781 tatgcgaatc cggtgcgcca tcccagatta attgtttcac gtccttgagc caggagccta
      841 cgggaagtcc cgcttcatga agtcgggctt tattaacatt aatatgaaaa ggctcctcca
      901 aagcgaatgc cagagaggga atacgatggt tgagtgccac cgccttgacg agaagagcgg
      961 gttcctggag caccgtaaag atgtgtgaat gatctcctgg aattggtgca ttgagttgac
     1021 acatgaatgc gtctttcgca tgaaaatgag ccgattggat gtgggaaggg tgaaattctt
     1081 tcacttcgag agttagcgga tatccgtcaa ctagattcca cgtatagccg cggagtttgc
     1141 cattgatatt ttcaataagg ccgggtggac catacaatcg aaggtgtttg ccccgaccaa
     1201 gggcaatacg aattagacgg tcaaatccga taaagtggtc catgtgtgta tgagagacga
     1261 aaatatcctg cgcccgtaga agctgggttg gatttaacga caaattctcg cccaaatcaa
     1321 agagcagcgc ccgacggctc caacgaacct caacatagag gccagggtca ccaaatggat
     1381 gattgaccaa acgcggtaaa aaagagggtg tcattctagt attccccgga taatgagata
     1441 aatcatcacc acctctacag catgagcggt cataatggca tagagattat gcatcttcat
     1501 ccacatccat ccccaaatca ggccgtccca caccgcaatc cagtgtaaat ggcggaatgt
     1561 tgtcaccaga aaaggatcaa aggaaaacgt gagagccgaa atgcctaacg ggagtatgac
     1621 ctgcataagg ttccgcatga cagagggatg agcggtttgg agaacatcat caaaaaaaac
     1681 aagtaaccgg cccaggacaa atccccggaa attcaattct accgccatgg caatgacgat
     1741 gataaaccag ggtgccatga tccagaaagg tatttgtgca tgaggcgttt tggagagaaa
     1801 actgatgtcc atctctaagg cgggaagggc atagagaatg attcctgtat tcatcgttcc
     1861 cagggagatg ccggttatgg tcccccaaaa aatccccggc tgaatgttgt caaatgacag
     1921 gccaagtttg acgagtcgac caacatttcg gcacgcccac acgcttaaag ccagataggc
     1981 aatcaattgg ggagtaaatt gaattgcgaa tggagtttgc caattaagag ggagcgcata
     2041 aaatccaatg gttgcaaaca ccggaatgaa ggctagtcct gcagctctct ctttgtgtgg
     2101 ggtcacattc ttgtccgtac cagagagggg acaagcgttc ttggcgtaaa tcggctgaat
     2161 tttccaaccc cattgatggt ggaaggtgag tctgggggat tcaatctaga ggcattcatg
     2221 gctagcaaga gaccggattc catcccaatc acggcagcag acacgatatc tattgcggca
     2281 ggttcaactt tggcccagaa gcctcctctg ccagactctt cttcatggtc ggaggcttgg
     2341 gtgcgctgct tagactcatg gccgccaatc atggagtgtc agggacaaga tgtcaggacg
     2401 ggcagcagga caagtcgaag agcagatagg cccatcataa tttagctcat acctgttctt
     2461 ttcaagaatc gagcagaaaa tgcccttcaa caaacttatc aaaccctgag attttccgca
     2521 atttttattg ttgtacagag aagaaaatat gtggcgggat tatcctgaca gaccccacaa
     2581 tgaccataca aaaagttgcc cggattgcca gagcaaaatc tgttcttggc tgactgactg
     2641 ttatgacctt gaggaagacg gcgtgtgtcc ccaagaaagg ggttgtggac ttccagtaag
     2701 aatgcgtatc atcgtaggcg ttatgagttg agccgattat caatttattt attttaggat
     2761 gggatggtca atgaaacagg agaggttaag ccttggacga tgtatttata ggagaatagt
     2821 tggtaagaag tttggggaga tacgagatcg tattgccgga ccagggctga tggtgatgat
     2881 ggtcctgagt ctgtttgggg ctgttggttc tgagggatat gctgatgatc caccgcagga
     2941 gcttcccaaa cctttccaca aaaccactcc tccacttttt caagaatgga attttgatca
     3001 gtctgcttcg aacgggtttc cggaagggtt cacgcaagct cttggcggga ttcaaggaga
     3061 gggggagtgg tcagtgaaag aagatccctc cgcccccagt cgtccgcgcc tggttgaaca
     3121 acgcttacac tgtgaagagg attggtgtta ccagctactt attgctgagg gagttgtagc
     3181 tgagtatgtg gatctttcag tccggatgaa actgaatctg gggaccccat ctggaaaagc
     3241 cggtttggct tatgggattc aagatgacaa aaatttctat gctgtcgttg tggagcccaa
     3301 aaccaacgag gtggtggcct atgtggtcaa aaatggacaa cccaccgaat tggcgcgaga
     3361 gtctcttatt cctcaacctt ccgattggca ttttcttcgg attcaacgta ataccattat
     3421 cagtaaggaa tttaccgaaa tattttttga tcaccatcta atgatcgatg tgtatgacca
     3481 atcatttaaa aaaggcaaat tg
//
LOCUS       DATIAL010000550         6642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1604169, whole genome shotgun sequence.
ACCESSION   DATIAL010000550 DATIAL010000000
VERSION     DATIAL010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604169"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VLA60_16830"
     CDS             <1..829
                     /locus_tag="VLA60_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HSF11079.1"
                     /translation="LSIGNLHCLLRGHEHMKAAQPSAPSRRPSKERLDSLVLTQELVE
                     SREQACRLILAGRVKVDGVLIDKPGKLVARTVSLEVTKPECAYASRAGEKLAPALDAF
                     CISCSGCVVMDVGASTGGFTDCLLQRGANRVYAIDVGYGQLDWKLRTDPRVVVMDRCN
                     IRHLCPQDIPEPVDLAVIDVSFISLRLVLPVILPVLREQAYLVALVKPQFEVGKGQVG
                     KGGIVRDERLREQVRDGFVDYARSLRLDVIGVMDSTLLGKKGNKEMLVGMKKREDEC"
     gene            888..1817
                     /locus_tag="VLA60_16835"
     CDS             888..1817
                     /locus_tag="VLA60_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HSF11080.1"
                     /translation="MSTKILVTGGAGFIGSHLVDRLIQEGNEVIVVDNLSTGKRKNVN
                     KKAQFYKMDIQSKRIERVFRNERPLIVVHLAAQMSVRHSTDDPGFDAQVNILGTINVL
                     EHAVKQGVRKVTFASSGGVVYGEQEIFPAAESHRTEPLSPYGISKLAGEKYLAYYANA
                     TGLRYVALRFANVYGPRQDSEGEAGVVAIFSKQMLDGGQPIVNGTGKQTRDFIYVDDA
                     VEAILVTLGEDVQGIFNVGTGQETTINECYGIIKSLTKCQCKELYGAAKKGEQFRSVL
                     DVTKLREVFGWDPQVTLQEGLTRTVDFFRGTGK"
     gene            complement(1868..2188)
                     /locus_tag="VLA60_16840"
     CDS             complement(1868..2188)
                     /locus_tag="VLA60_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSF11081.1"
                     /translation="MSYTITVSELKARMDKGDKIFLLDVREPHEYSLAKIEGSVLIPL
                     GQVPHSLKQLDPSAEIVAYCHKGMRSADAVGFLLQQGFSNVKNLIGGIEAWSIEIDQS
                     VPRY"
     gene            2301..3602
                     /gene="asnS"
                     /locus_tag="VLA60_16845"
     CDS             2301..3602
                     /gene="asnS"
                     /locus_tag="VLA60_16845"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HSF11082.1"
                     /translation="MPEQSVVSIEQISQFEGQDITIKGWIRNRRSSGKLSFLTVRDGT
                     GDLQAILSKGEVGDEQFSLCSQLTQESSVILTGKPRRDARAPGGFELDVSTLAVIHLA
                     EPFPIQPKDHGVGFLMEYRHLWLRSRRQHAILRVRHEIIRTCRNFFDDRGFTLIDTPI
                     FTPNACEGTTTLFQTQYFDQVAYLAQSGQLYGEAAAAAFGKIYCFGPTFRAEKSKTRR
                     HLMEFWMVEPEMAFADLPDAMQLAEELVSTVIQRVVETRRPELEILERDIPKLEAMAK
                     PFPRITYEEALTRLQHKGVALQFGDDFGADDETSLAQEFDRPVIVHRYPAAAKAFYMA
                     KDPERPDLALCMDMLAPEGYGEIIGGGQRIHDLDELLTQIKAHDLPEEAFKWYVDLRR
                     YGTVPHAGFGMGIERVVAWICGLDHVRETIPFPRMLYTLYP"
     gene            3795..4709
                     /gene="rsmH"
                     /locus_tag="VLA60_16850"
     CDS             3795..4709
                     /gene="rsmH"
                     /locus_tag="VLA60_16850"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HSF11083.1"
                     /translation="MHDVHIPVLVEEICFWLNPLPGGIYVDCTVGIGGTSLKILEKTG
                     KNAHIIGLDRDYQAVALAKKTLSEYESSVNILHGNFSHIRDFVQKSGYEKVDGVVFDL
                     GVSSLQLDQAERGFSFSLSGPLDMRMDQTQKVTAADLVNHLPEKELADVIFTYGEERY
                     SRRIARAIVQARGAGLIQTTQALVSVLEGALPFAYKKGRLHFATRTFQALRIRVNREL
                     DLLEPALRDAIDLLKDGGRVCVVAFHSLEDRIVKQTFRSLAQREHPKVALLVKKPVIP
                     SVSEIQQNPRARSAKLRVAERLPEGETL"
     gene            4706..5053
                     /locus_tag="VLA60_16855"
     CDS             4706..5053
                     /locus_tag="VLA60_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11084.1"
                     /translation="MRKNVFLGVMILLGVWCLLYVGVKIDMWRLGYAIEELETQRAVF
                     KKQQEGLQVRLSQLTDPQRVAQRAQSQLGLTVAQEGQIVMVSLDTLPQSESDGNFPVR
                     LVREFGNTVVSLP"
     gene            5050..>6642
                     /locus_tag="VLA60_16860"
     CDS             5050..>6642
                     /locus_tag="VLA60_16860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HSF11085.1"
                     /translation="MKTPSQDIHPVCRIRSGIVCLGLLCGFVLIGCRLFYLQVLQADV
                     GAHQAQQQHEKTVIVQPDRGVIVDRQGHPLALNVEVSSMYVRPPSLNDPQRVARSLAP
                     ILQMPVKELRDLFTRNQPYVWVKRNIPEPVVKEVEALNISGLGLTKEPHRFYPKGELV
                     SHLVGFAGIDSQGLEGVELQYESYLRGEKNLVKYQRDALGRKLASPPSHDSVPLSTGY
                     HLTLTIDEVVQFIAEAELAQALKQSGAKSGSIVIMDPLTGAILAWALHPTFDPNHLGQ
                     FSTQDWRNRLVTDPYEPGSTLKIVVAAAALEEQVMAPDTLIYGGDGKMSVAGTIVHDP
                     AKTSWMTFSEALAQSSNVGAIKVAMELGQDRVFRYLKAFGFGEKTEIDLPGESSGILR
                     EPNKWSGRSLSSIAMGQEIAVTPIQLVTAMSVVANGGWLMTPYVVSSILDSNGQAVLT
                     KDPQPKRRPISLETTDTLTRILELAVEAGTGKRARLAGYRAAGKTGTAQKIDPKTGSY
                     SSSQVIASFVGFAPVERPRLAML"
BASE COUNT         1642 a         1426 c         1837 g         1737 t
ORIGIN      
        1 tctctcaatc ggtaatttgc attgtctgct tcgtggacat gaacatatga aggctgctca
       61 gccatcagct ccatctcgga gaccctcaaa agaacgactg gattcccttg tcctgactca
      121 ggaactggtc gaaagcagag aacaggcctg ccgacttatt cttgccggtc gagtgaaagt
      181 cgatggggtc cttattgata agccagggaa gctggttgcg agaactgttt cgctggaagt
      241 gacaaaaccg gaatgcgcct atgccagccg agccggtgaa aagctggcac ctgccttgga
      301 tgcattttgt atttcctgtt caggctgtgt ggtgatggat gtgggtgcct ccactggagg
      361 gttcacagat tgtctgctgc aacgtggcgc caatcgagtc tatgccattg acgtaggata
      421 tgggcagtta gattggaagc tgagaacgga tcctcgtgtt gtggtcatgg accgttgcaa
      481 tatccgccat ctgtgccccc aagatattcc tgagccggtt gatttggcgg taatcgatgt
      541 ttcatttatt tccctacgtt tggtattgcc agtgattttg cccgtcctgc gtgaacaggc
      601 gtatcttgtc gcattggtca agccgcagtt tgaggttggg aaaggacaag tcggaaaggg
      661 aggcattgtc cgtgatgaac gattgcgtga gcaggtgagg gacggttttg tggattatgc
      721 ccgtagcctt cgtctcgacg tcattggtgt catggattcg accctcctgg ggaaaaaagg
      781 aaataaagag atgttggtgg gaatgaaaaa acgggaagat gagtgctgaa ggacgcaagc
      841 caattagtat ccccttaacc taatcatgta aaaggagaaa gccgggcatg tctacaaaaa
      901 ttctggtgac cgggggagct ggattcattg gatcacatct ggttgaccgg ttgattcagg
      961 aaggcaatga ggtgattgtt gttgataacc tctctacagg gaaacgaaag aatgtcaata
     1021 aaaaggctca attttataaa atggatattc agagtaagcg gattgaacgg gtattccgaa
     1081 atgagcgtcc ccttattgtc gtccatctgg ccgcgcagat gagtgttcgc cattccacgg
     1141 atgatccggg gtttgatgcg caagtcaata ttttagggac catcaatgtt ttagagcatg
     1201 ccgtgaagca gggtgtgcga aaagtgacat ttgcatcgtc gggtggtgtg gtctacgggg
     1261 aacaggaaat attcccggct gcagagtcac accggacaga acctctttct ccttatggaa
     1321 tcagtaagct ggctggagaa aaatatctgg cctattacgc aaatgcgacc ggactccggt
     1381 atgtggcgct tcgttttgcg aatgtttatg ggcccaggca ggactctgag ggggaggcag
     1441 gggtcgtggc tattttctca aaacaaatgc tggatggtgg tcaaccgatt gtcaatggaa
     1501 ctgggaagca gacaagagat tttatctatg tagatgatgc ggtggaggcg atcttggtga
     1561 ccttgggaga ggatgttcag ggaattttta atgtgggaac tggccaggag acgactatca
     1621 acgaatgtta tggaattatt aaaagtttaa cgaagtgcca atgcaaagag ctatacggag
     1681 cggcaaaaaa aggcgagcaa ttccggagtg tgttggatgt gaccaaactc agagaggtat
     1741 ttggctggga tcctcaggtt actttgcagg aagggctcac ccggacagtc gatttttttc
     1801 gtggaaccgg gaaataacct ctccgttgcg cgaagaagga gagtgatttc acctgatagc
     1861 ttctctgtta atacctggga acggattgat cgatctcaat ggaccaggct tcaattcctc
     1921 caatcagatt tttaacattc gagaatccct gttgcaaaag aaaccctacg gcatccgcac
     1981 tccgcattcc tttatggcaa tacgcgacaa tctcagcgct cgggtccaat tgtttcaggg
     2041 aatgggggac ctggccaaga ggaatcagca ctgatccctc tatttttgcc agggagtatt
     2101 cgtggggttc tcggacatcc aacaagaaaa ttttatcgcc tttatccatg cgagctttta
     2161 attcgctgac agtgatggtg taactcatat ttgaaggcct ccttacgttc ctgaggtatc
     2221 tcttgatcga caaatgatca tagcggctga attttcaggg tgtcaatttt ttctttgtga
     2281 gagcgggagg gagcagcatc atgccggaac aatcggtcgt ctcgattgaa cagatttctc
     2341 agtttgaagg gcaggacatt accatcaagg gatggatccg caaccgacgc tcgagcggaa
     2401 aattaagctt tttgacggtg cgtgatggca caggagatct ccaggctatt ttaagcaaag
     2461 gtgaggttgg cgacgagcaa ttttctttgt gtagtcagtt aacccaggag tcttctgtga
     2521 ttctgactgg aaagcctcga agggatgctc gagcgccagg tggatttgag cttgatgtat
     2581 ccacattggc agtgattcac cttgcagaac cctttcccat ccaacccaaa gaccatggag
     2641 ttggtttcct gatggagtat cggcatctct ggttgcggtc gcgacgccag cacgccattt
     2701 tacgggttcg ccatgaaata atccggacgt gtcggaattt ttttgatgat cgaggtttta
     2761 ccttaattga tacgccgatt tttaccccca acgcctgcga gggcaccacg accttatttc
     2821 aaactcaata ttttgaccag gtagcctatt tagcccaaag cggtcagtta tatggggaag
     2881 cggctgctgc ggcttttggg aaaatatact gttttggtcc aacctttcga gcggaaaaat
     2941 caaaaaccag acgccatttg atggagtttt ggatggttga accggaaatg gcctttgccg
     3001 acctaccgga tgccatgcaa ttggcagagg aactggtttc aacggttatt cagcgagtcg
     3061 tagaaactcg tcgacctgaa ctggaaatct tagagcggga tattcctaaa ttagaagcca
     3121 tggccaaacc ctttccgcga attacctatg aagaggcact gacgcgtctt caacacaagg
     3181 gggtcgccct tcaattcggc gatgatttcg gagccgatga cgaaacatct ttagcgcaag
     3241 aatttgatcg tccggtgatt gttcatcgat atccggctgc cgccaaagca ttttatatgg
     3301 ctaaagatcc agagaggcca gacttggcgt tatgtatgga tatgctcgcg ccggagggat
     3361 atggagaaat tattggagga ggccaacgaa tccatgattt ggatgaattg ctgacgcaaa
     3421 ttaaagctca cgaccttcct gaggaggcct ttaagtggta tgtggatttg cgacgatacg
     3481 gaacggttcc tcatgctggt tttggtatgg gaattgagcg agtggtcgcc tggatttgtg
     3541 gattggacca tgttcgagaa acgattccat tccccagaat gctctatacg ttgtatcctt
     3601 aaaatatttt tttggaatga ccttgaggtt ctcgaatatt ttggccatgg ctcctaaatt
     3661 ttttattacc tagctcctac tttcttacaa cattttttga gtgcttgaca tcataggcga
     3721 gggttgtata cttccagatg tgggagaaag tgggatgatt tccacatata ttgtaaaaat
     3781 gaaacaagcc gtgcatgcat gatgttcata taccggtact cgtagaggaa atctgttttt
     3841 ggcttaatcc gctgcctggc gggatctatg ttgattgtac tgtgggaatt ggtggaacgt
     3901 cgctgaagat actggaaaaa accggaaaaa atgctcatat tattggtttg gaccgtgatt
     3961 atcaggccgt tgctcttgcg aaaaagacat taagtgaata tgaatcgtct gtaaatatac
     4021 tgcatgggaa tttctcccac attcgagatt ttgttcagaa gtccggctat gagaaggttg
     4081 acggggtggt gtttgattta ggggtgtcct cgttgcagtt ggaccaggca gagcgtggat
     4141 ttagtttttc cctgagtggg ccattggaca tgcgaatgga tcaaacccag aaagtaacag
     4201 cagcagatct ggtgaatcat cttccggaaa aagagttagc ggatgtcatc tttacctatg
     4261 gtgaagaacg gtattcccgt cgaatcgccc gtgcaatcgt gcaggcccga ggcgccggtc
     4321 ttattcagac cacgcaggcc ttggtgtctg tccttgaggg cgcgcttcct tttgcctaca
     4381 aaaaggggcg cttacatttt gcgaccagaa cctttcaggc gcttcggata cgcgtgaatc
     4441 gtgaattgga cttgctcgag ccggctctcc gggacgccat cgacctgttg aaggatggcg
     4501 gaagggtgtg tgtcgtcgca tttcactcgt tagaagatcg tattgtaaag cagacgtttc
     4561 gttctctggc gcagagagag catccaaaag ttgcattgct ggtcaagaag cctgtgattc
     4621 cgagtgtgtc ggaaatacaa caaaatcctc gtgccagaag cgctaagtta cgtgtggccg
     4681 agcgccttcc ggaaggagag accctatgag gaaaaacgtg ttccttggcg tcatgattct
     4741 attgggagtg tggtgtctgc tctatgttgg ggtcaagata gacatgtggc gactggggta
     4801 tgccattgaa gagttagaaa ctcagcgtgc ggtattcaaa aaacagcaag aaggcttgca
     4861 ggttcggctg tcgcagttaa cggatccgca gcgagttgcc caacgggcgc aaagtcaatt
     4921 gggactcacc gtggctcagg aagggcagat tgttatggtc tccctggaca cgttacccca
     4981 gtctgaatcg gatggaaatt ttcctgttcg tttggtccgg gagtttggca ataccgtcgt
     5041 gagccttccg tgaaaacacc atctcaggac attcatcccg tctgtcgaat ccgaagtggc
     5101 attgtctgct tgggcctcct ttgcgggttt gtgcttattg gttgccggct gttttaccta
     5161 caggtccttc aagcggacgt tggggctcat caggcacagc agcaacacga aaaaaccgtg
     5221 attgttcagc cggatcgagg ggtcattgtt gatcgacaag gtcatccact ggctctcaat
     5281 gtggaagtgt cctcaatgta tgtgaggccg ccgtccttga atgatccaca acgggttgcc
     5341 cgatccttag cgccgatctt acaaatgccg gtcaaggaat tacgcgattt gttcacacga
     5401 aatcagccat acgtgtgggt taaacgcaat attccggaac ccgtcgtcaa ggaagtggag
     5461 gcgttgaaca tatccggatt gggattgacc aaagaaccgc atcgttttta tcccaaggga
     5521 gaattggtat ctcatctcgt ggggtttgcc ggaattgata gccagggatt agaaggggtg
     5581 gagcttcaat atgagtccta tctacggggt gaaaaaaatc tggtgaaata ccaaagggac
     5641 gcgttgggca gaaagcttgc ctcaccacca agtcatgatt ccgtaccctt gtcgaccggg
     5701 tatcatctca ctttgaccat agatgaggtt gttcaattca ttgcggaggc ggagttggcg
     5761 caagccttaa agcaaagcgg cgcaaagtct ggaagcattg tgatcatgga ccccttaacc
     5821 ggagcgattc tggcgtgggc acttcatccg acctttgacc ctaatcactt agggcagttt
     5881 tccactcagg attggcgaaa tcgactggtg acggatcctt atgaaccggg gtcaacgttg
     5941 aaaattgtgg tggcagcggc ggcgcttgaa gagcaggtaa tggctccaga cacgttaatc
     6001 tatgggggtg acggaaagat gtcggtggca gggaccattg ttcatgatcc ggcaaaaacc
     6061 agttggatga cgttttccga agcgctggcg caatcgagta atgtgggtgc gatcaaagtc
     6121 gccatggaat tagggcaaga ccgggttttt cgatatttaa aagcgtttgg atttggagaa
     6181 aaaaccgaaa tcgatcttcc gggagagtcc tcaggaatac tccgagagcc caataaatgg
     6241 agtggtcgaa gcctttcttc tattgctatg ggtcaagaaa tcgccgttac cccgattcaa
     6301 ttggtcacag ccatgtcagt ggtggccaat ggtggttggc tgatgacgcc ctatgtcgtc
     6361 tcttcgatcc tggattcaaa cggacaggct gtcctgacca aagatccgca acccaaacgg
     6421 cgacccattt cgcttgagac aaccgacacg ttgactcgga ttctcgaact ggctgttgag
     6481 gcaggaacag gcaagcgtgc caggcttgcc ggatatcgcg ctgcgggaaa gacggggacg
     6541 gctcaaaaaa ttgacccgaa aaccgggtcc tactcttctt cgcaagtgat tgcttcgttt
     6601 gtcggatttg caccagtcga gcgcccgcgt cttgccatgt ta
//
LOCUS       DATIAL010000551         9284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_850445, whole genome shotgun sequence.
ACCESSION   DATIAL010000551 DATIAL010000000
VERSION     DATIAL010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 9284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9284
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850445"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..679)
                     /locus_tag="VLA60_16865"
     CDS             complement(50..679)
                     /locus_tag="VLA60_16865"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HSF11086.1"
                     /translation="MSPKIKICGITNQKDAEFAVQEGADALGFVFYAQSPRYVQPAIA
                     QHIIANLPPFVVTVGVFVNHDLDMVKRVFDDCGLSLAQLHGDESPGFCESLQRPVLRA
                     IRLKDRRSYLALAEWKGRIGVRGFIIDAFSPTAYGGTGHTTDWSLAGEVARAVPMLLA
                     GGLTPENVQEAICQVQPYGVDVSSGVEQSPGRKDPAKIRDFIQSVRLVC"
     gene            complement(835..1680)
                     /gene="trpC"
                     /locus_tag="VLA60_16870"
     CDS             complement(835..1680)
                     /gene="trpC"
                     /locus_tag="VLA60_16870"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HSF11087.1"
                     /translation="MILSRILEHKKAELRRKQSRGYLAELKGRIVDRTRPLGFIQALE
                     AGLTSTAPALIAEVKKASPSQGLMRPEFHDQFEPVTIASQYRDHGASALSVLTDQDFF
                     QGNLEYLHNVKEAVHLPTLNKEFMVEEIQFYEARAYGADCVLLIAAALDRFQLEDFFT
                     VAQELSLDVLIEIHNERELDTVLERVPLARLIGINNRDLNTFQTDLSVTERLAKRLPA
                     DTLIVSESGIHNRKHVERVLEAGAKAMLIGESLLRADSIQAKIQELLHPPSPPKKEES
                     TTRWV"
     gene            complement(1677..2702)
                     /gene="trpD"
                     /locus_tag="VLA60_16875"
     CDS             complement(1677..2702)
                     /gene="trpD"
                     /locus_tag="VLA60_16875"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HSF11088.1"
                     /translation="MPLLKDHIAKLAEGLDLSELEAEEAMREIMQGGATEAQIAAYLM
                     GLRMKGETIDEITGSVRAMRERAIRIPIADPQVVDTCGTGGDKSHTFNISTAAAFVVA
                     GGGMTVAKHGNRSVSSQSGSADVLAALGVNIDLSPEKVADCINEIGIGFLFAPLYHGA
                     MKHCAKPRSELGIRTLMNIMGPLANPARATIQILGVYDQTLTEKLAQVLVRLGTQHCF
                     VLHGMDGLDEISLTSRTGIAEGKKGRVLSYFIGPEDFGLHPVSPKELLGGNSEDNAQI
                     IRDIFRGRMGPKRDIVLMNAAPAFIACQKATSLKEGFEEAGRVVDNGAAFEKLEKLIS
                     LTKKLAA"
     gene            complement(2743..3330)
                     /locus_tag="VLA60_16880"
     CDS             complement(2743..3330)
                     /locus_tag="VLA60_16880"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HSF11089.1"
                     /translation="MILVIDNYDSFTYNLVQYLGELGADLKIFRNDALTIDDIHRLAP
                     ERILISPGPCTPNEAGISVAIIKHFAGRLPLFGVCLGHQSLAYAFGGQIIRAPRLMHG
                     KTSMVHHDGKTIFEGLPNPFEATRYHSLIVKRETLPADFEISAETVEGEIMGLRHTPS
                     GAEGVQFHPESILTTAGMDLLRNFLLLPVCSSTDH"
     gene            complement(3351..4847)
                     /gene="trpE"
                     /locus_tag="VLA60_16885"
     CDS             complement(3351..4847)
                     /gene="trpE"
                     /locus_tag="VLA60_16885"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HSF11090.1"
                     /translation="MKNAYYSVSFDEFCQFASQGNLVPLYREILADFETPVSAFSKIN
                     TGANAFLFESIEGGENWARYSFLGSHPSVMLWEENGEVVTQKGRKQRRVPLQGNPLDH
                     IQNTMAEYHPVVVPGLPRFVGGAVGYLGYDVVKSFEPVPSRGKPGIKTPLFAFCITDT
                     LLIFDNVAHTLKVVANAHITSAKKTQLRQIYQDAIKRIESIIARLHKPSRRPTASIRR
                     APLKFQSNLSPEDFEKMVLRTKEYIQAGDIIQGVMSQRWQTTIHTDPLEIYRALRVLN
                     PSPYMFYLRIAGIELVGSSPEILVRCEEGNIVVRPIAGTRPRGQTPEADNALEQDLLS
                     DHKELAEHVMLVDLGRNDVGRVAKAGTVKVERFKKVERYSHVMHIVSQVKGELDPQYT
                     AYDVMKACFPAGTVSGAPKIRAMQIIEELEPTRRGPYAGAVGYFSFSGNMDTCINIRT
                     VVVQGQKAYIQAGAGIVADSDPTREYEETRTKAGAMMRAIEMAEHGLA"
     gene            complement(5000..5680)
                     /locus_tag="VLA60_16890"
     CDS             complement(5000..5680)
                     /locus_tag="VLA60_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSF11091.1"
                     /translation="MNLRGGTRNMVQPLFGGHQGDPQSSGWFGYLKIVAGVMVCSGFL
                     SGCVSTEKFEAEKARALNFQRLLAQEEKRTGELNVKYQDTQRQMGSLESQNRDLNAEL
                     EALRDQLNRSHDELSRVREGGMGKSDDLKLSEPSISEFGLNDLDFKDSDMTKLDSDLN
                     LGDSIPLDSPSMDTMGMHEGSHIVAKGETLYRISKEYGVSIADLKAWNQLTDNTIHVG
                     QKLIVSGQ"
     gene            5928..6845
                     /locus_tag="VLA60_16895"
     CDS             5928..6845
                     /locus_tag="VLA60_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HSF11092.1"
                     /translation="MHIGQVALRLARPLTHTQAWMGDQEILRQLLACWFIVDEKDVPL
                     SPRIIGQPGVGKTTLAMAATQERKQELFIYQCTADTRPEDLLVSPVISEGGTITYQAS
                     PLVTAMVTGNVCLLDEGNRMNEKSWASLASLLDHRRSVDSVVAGVQIHAHENFRCCVT
                     MNEDASTYEVPDYILSRLQPTLKVEFPNKEHELAILRYHLPFASAELITLTVNFLQKA
                     HQLDLPFSIRDGMHMVQYAMKRMAQDPHHPVARDPAWREALINVLGEEAQDLDVLAKR
                     RSQTLHGHALPKGLGDFFFEEDHPLHPDQ"
     gene            6941..8836
                     /locus_tag="VLA60_16900"
     CDS             6941..8836
                     /locus_tag="VLA60_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11093.1"
                     /translation="MTLSSLIPDTIFALSSNIHVLPVVHGSGDMAHVVREIIVSRHID
                     CLVLPLPASVQSLIEKGIDQLPVISLVVLPEQHDDGTPSCSYVPIDPCQPVIMGIRSA
                     MSEMIPRAFIDREVRRYHPVSWVGPDPYTLKAVPLAAFSAATMPFLPPPQDPSSRWER
                     IRWMAFRLHELELDFSAILFLCPMTDWPWLRQAYQERMPYISPEQNISLPEWWKVEPS
                     SLYFALSELPYVTHLYEQRREEARADTHLAIDGIKELVLEARARWLASRSTTIAHETN
                     WVTPQLLQRYFQYVRNLTLLEHRLKPDLYTLVLAAKQMAGDEFALRLLETAKTYDYQT
                     QPSVLDTRPGVLVGIGELQDPGGNILPAVNRLQGDPLVWRRVTLRPEPPRPKTQAWAQ
                     EWNPYRQCSWPPEDQRIESFAAHVRQHSRQVLGADLGRIERFTNSLEDGIDLRATLRQ
                     WAMTPQRSVRDIHVKVVPPVRGTIESLVFFFEVPADPQKFSWQTTWYAEHQEESTLSF
                     YATPFSPNMVGPGIGQACYGGALFLYPPRLIPDIWENPLFDFATSLEERLLAGACAHS
                     QEPVVAVVSPVPLTWAWRKTARRFGRKLLPIPLHRFSGQTIARLRQFHVLNGHEIRSY
                     AAKFIRE"
     gene            8811..>9284
                     /locus_tag="VLA60_16905"
     CDS             8811..>9284
                     /locus_tag="VLA60_16905"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11094.1"
                     /translation="MQPNLFENNPDEAGGEDSFKAPQSRAVPSAKVQSKLEKLKHAIR
                     RHDELYYVRSRPEISDAEYDQLFRELQDLESQYPDLRTPDSPTQRVGGAPLAQFSKIT
                     HEFPLLSLDSEMDEARVLAFDQRVCRELDVRQASYSAEPKYDGLSVALTYDHGIFV"
BASE COUNT         2216 a         2421 c         2269 g         2378 t
ORIGIN      
        1 gttctactga aagaggacgt aaaaagaaaa gagtatagcg atggaatggc taacacacaa
       61 gacgaaccga ttggataaaa tcccgaattt tggccggatc tttacgccca ggactctgtt
      121 ccacaccgct actcacatca accccatagg gttgcacttg gcaaatcgct tcttgaacat
      181 tttcaggagt taatcctcct gccaacagca tgggaacggc tctggctact tcgcccgcaa
      241 gtgaccagtc ggtcgtgtgt ccggttcctc cgtatgcggt aggagaaaat gcatcaatga
      301 taaatcctct cacgcctatc cgtcctttcc attctgccaa tgcgagatag ctacgcctat
      361 cttttagccg aatggcgcgt aggaccggac gctgtaacga ttcacagaaa ccgggagatt
      421 cgtcaccgtg aagttgtgcc agactcaatc cgcagtcatc aaatacgcgt ttcaccatat
      481 cgagatcgtg attgacgaag acacccactg tcaccacaaa agggggaagg ttggcaatga
      541 tatgttgggc gatagcaggt tggacatatc gagggctttg ggcgtaaaac acaaaaccca
      601 acgcgtcggc cccttcttga acggcgaatt cggcatcttt ctgattcgtg atgccgcaaa
      661 tttttatttt aggggacata ggaacattcg ccgtttgttg gggacattcg cagacaccac
      721 agtatatggc ttcagactca tttctctgcc ttctttacag aggcatctat ataaattcgg
      781 ccggtctttc gtataagaga acaggaacag tcgagagtcc cccgcaatcg aatcctagac
      841 ccaacgagtg gtggattctt cttttttggg aggtgacggt ggatgtaata attcctgaat
      901 tttggcttga atcgagtctg cgcgcaataa cgactcgcca atcaacatgg ctttggctcc
      961 ggcttccagt acccgctcca cgtgcttacg gttgtggata ccactttcac tgacaataag
     1021 tgtgtccgcc ggcagacgtt tggccagccg ctcggtgaca ctcaaatcag tctgaaaggt
     1081 gttgagatcc cggttgttga tgccgataag tctggccaag ggcacccgtt caaggacagt
     1141 atccaactcc cgttcattat ggatttcaat gagcacatcg agcgacaact cttgggcaac
     1201 tgtaaaaaaa tcctcaagct ggaatcgatc caacgcggcc gcaatcagta aaacacagtc
     1261 tgctccatag gcccgcgctt cataaaattg aatctcctca accataaatt ctttattgag
     1321 cgtgggaagg tgaaccgcct ctttgacatt gtgcaaatac tctaagttcc cctgaaagaa
     1381 atcctgatcc gtgagaacgg aaagggcact cgctccatga tctcggtatt gagaggcgat
     1441 ggtcactggc tcaaactgat cgtgaaattc cggtcgcatc aggccttgac tgggcgaagc
     1501 ttttttgact tcggcaatta atgccggagc tgtcgacgtt agtcctgcct ctaatgcttg
     1561 aataaaaccc aggggtcgcg ttcgatcaac aatgcggccc ttaagttcag ccaggtaccc
     1621 ccgactttgt tttcgacgca attcggcttt tttatgttcg agaatacgag aaagaatcat
     1681 gccgccaact ttttggtcag ggaaatgagt ttctccaact tttcaaatgc ggctccgttg
     1741 tccaccacac gaccggcttc ttcgaatcct tctttcagcg aggtcgcttt ctggcaggcg
     1801 ataaaggctg gcgcggcatt catcaacacg atatcccgtt tggggcccat tcgtcctcga
     1861 aaaatatcgc gaataatctg ggcattgtct tccgagtttc ctcccaacag ttctttgggc
     1921 gaaaccgggt gaaggccgaa atcttccggc ccgatgaaat agctcagtac tcgtcctttt
     1981 ttcccttcag caatccctgt gcgactggtt aatgaaattt catccaaccc gtccatccca
     2041 tgaaggacaa aacaatgctg tgtgcccaac cttactagaa cctgagccaa cttttcggtg
     2101 agggtttgat catacactcc cagaatctga atcgtggctc gtgccggatt ggccagcggt
     2161 cccatgatat tcatcaaggt acggatgccc agctccgacc gaggtttggc gcaatgcttc
     2221 atggctccgt gataaagcgg tgcgaagaga aacccaatgc caatttcatt gatgcaatcg
     2281 gcaacctttt caggggacaa gtcgatattc acaccaaggg cggccagcac gtccgcactc
     2341 cctgattgag aggacacaga gcgattccca tgcttggcaa ccgtcatccc ccctccagcc
     2401 acgacaaagg cggcagccgt cgagatattg aacgtgtggc ttttgtcccc accggttcca
     2461 cacgtatcca ccacctgagg atcggcaata gggatccgaa tcgcccgttc gcgcatggct
     2521 cgcactgatc cggtgatttc atcgatcgtt tcgcctttca tgcgtaatcc cattaagtag
     2581 gctgcaattt gggcctccgt ggctcctcct tgcatgattt cccgcatggc ttcttccgct
     2641 tcgagctctg agaggtcgag gccctcggct aatttggcga tgtggtcttt gagtaggggc
     2701 atgtgaaaag gctcatggac agatgactgg gaagtcttgg atttagtggt cggttgaaga
     2761 gcagacgggt aataataaaa aattccgcaa aaggtccatc cctgccgtgg tgagaatcga
     2821 ttcagggtga aattgcacgc cttcggctcc gcttggggta tgtcgaagtc ccatgatttc
     2881 gccttccacg gtttcggctg aaatttcaaa atccgcgggt aaggtttcac gcttgacgat
     2941 taaggaatga tatcgggtgg cttcaaaggg attcgggagg ccttcaaaaa ttgtttttcc
     3001 gtcatgatga accatggagg ttttcccgtg cataagtcta ggggctcgaa taatttgacc
     3061 tccaaacgca taggccaggg attgatgccc gagacagaca ccaaagaggg gaagacggcc
     3121 ggcaaaatgc ttgatgatcg caacagaaat gcccgcctcg ttaggcgtac agggtcctgg
     3181 ggatattaat attcgctcag gcgccaggcg gtgaatgtca tcgattgtga gggcatcatt
     3241 tcgaaaaatc ttgagatcgg ctcccaactc tccaagatat tggacaagat tgtaggtaaa
     3301 cgaatcgtaa ttgtcgatga ccaggatcat ggggttcttc tcaagccttt ttatgctaga
     3361 ccatgttcgg ccatttcaat ggctctcatc atggcgccgg cttttgttcg agtttcttca
     3421 tattcacggg tagggtcaga atcggcgaca attccggcac cggcctgaat atacgccttt
     3481 tgtccttgaa cgaccaccgt acgaatattg atacaggtat ccatatttcc ggaaaaacta
     3541 aaatacccga ccgctcccgc ataaggtccc cgacgagtgg gttccaactc ctcaatgatt
     3601 tgcatggctc ggattttcgg cgcaccggac acggtgccgg ccggaaaaca cgccttcatg
     3661 acatcatagg cggtgtattg cggatctaat tctcccttga cttgagacac aatgtgcatg
     3721 acatgggaat accgttcaac tttcttgaac cgttcaactt tgactgttcc ggctttggcc
     3781 actcgaccca catcattgcg gcctaaatcc actaacatga catgttcggc taattccttg
     3841 tgatcggata agagatcctg ttccaatgca ttgtcagctt ccggcgtttg gccacgggga
     3901 cgggttcctg caatgggtcg caccacaatg ttgccttctt cgcatcgcac aagaatttcc
     3961 ggagaagatc cgacgagttc gatgccggca atgcgtaaat agaacatgta gggtgaggga
     4021 ttgagcaccc tcaaggctcg ataaatctcc aggggatcgg tgtggatagt ggtctgccag
     4081 cgttgggaca tgacaccctg gatgatatcc ccggcctgaa tgtattcttt agtccgaagc
     4141 accatttttt caaagtcttc aggagacaga ttcgattgaa atttgagtgg cgcacgtcga
     4201 atcgaggccg taggtcgccg gcttggttta tgaagtctgg caatgattga ttcaatccgc
     4261 ttgatggcgt cttgatagat ctggcgaagt tgggtcttct ttgcagaagt aatatgggca
     4321 ttggccacga ctttgagggt atgggcgaca ttgtcaaaaa tgagtaaggt gtccgtaatg
     4381 caaaacgcaa agaggggagt tttaatgccg ggtttccctc gagacggaac cggctcaaaa
     4441 gacttgacca cgtcataacc caaataaccc accgcaccac caacaaatcg tggcaaaccg
     4501 ggaacgacaa ccggatgata ctctgccatg gtattctgaa tgtggtccaa ggggttgccc
     4561 tgcagcggaa cgcggcgctg tttccggcct ttttgggtga ccacttcgcc gttctcctcc
     4621 cacagcatga cggaagggtg actacccagg aaggaatagc gtgcccagtt ttccccccct
     4681 tcgatacttt caaataaaaa ggcattggct cccgtattaa ttttggaaaa tgccgacaca
     4741 ggcgtctcga aatccgccag aatttctcga taaaggggga cgaggtttcc ttgtgaggcg
     4801 aattggcaaa attcatcaaa actcactgaa taataagcat ttttcatgga tccgacggta
     4861 gcatatggcc tatgaggtgt caacgaaagt gccgtgaggc acaggtcttc tcgagggaag
     4921 aataagaaaa ggccggaacc gaaggaagcg gctttccttg ggattccgac cttagcttga
     4981 agaatgaatg gaggtgaaat tactgaccgc tgacgataag cttttgtcca acgtgaatgg
     5041 tgttatccgt caactgattc caggctttga gatcggcgat cgacacgccg tactctttgg
     5101 aaatccggta gagggtctcg cctttcgcta caatatgtga cccttcatgc attcccatag
     5161 tatccatact cggtgaatcc aaaggaatgc tgtcccccaa attcaagtcc gaatcaagtt
     5221 tcgtcatatc tgaatcttta aagtcaagat cattcagccc aaactcagag atcgaaggtt
     5281 cggaaagttt cagatcatcc gattttccca tccctccctc tcttacacgc gatagttcgt
     5341 catgggaacg gttcaattgg tcgcgcaacg cttctaattc ggcatttaaa tctcggtttt
     5401 gggactccaa tgatcccatt tgtcgttgcg tgtcttgata cttcacattt aattcaccgg
     5461 ttcgtttttc ttcttgcgct aaaagccgtt ggaaatttaa cgcacgcgcc ttctcggctt
     5521 cgaacttttc cgtgctgacg catccggaca agaatccact acagaccatc acgccagcta
     5581 caattttcag atatccaaac cagcccgatg attggggatc tccttgatgt cccccgaaca
     5641 atggttgcac catattacgc gttcctcctc tgaggttcat atgtacccct cctttaggga
     5701 caatggtcaa taacgagaag gcgaagttct ccaccatctg gtcacctttt ggtacacaat
     5761 aattcggaat agggataatg tcaaacaact cttttgcaca ccggtagaag ttaccgaaca
     5821 ccacgattca ttgaccatcg aaaaatggct gtgataattt cacgtaccat ttgccttcgt
     5881 ccttttctat gacctctcca ttttcacatt ctgcagatcc ttcggttatg cacatcgggc
     5941 aggttgcgct tcgcctggcc cgtcccctca cacacacgca agcctggatg ggcgatcagg
     6001 aaattttacg acagcttctc gcctgttggt ttatcgtgga tgaaaaagat gtgcctttat
     6061 caccgagaat tatcggtcaa ccgggcgtgg ggaagaccac actcgccatg gcggccactc
     6121 aggaacggaa gcaggaactc ttcatttatc aatgtacggc ggataccaga cctgaagacc
     6181 ttctcgtctc cccggtcatt tctgagggag gcaccattac ctatcaagcc tctcctttag
     6241 tcaccgctat ggtgaccggc aatgtctgtc ttcttgatga aggcaaccgg atgaatgaaa
     6301 agagttgggc ctcccttgcc tccctgcttg atcatcgacg ctcggtcgat tcggtcgtag
     6361 cgggtgttca aattcatgcg catgagaatt ttcgatgttg tgtcacgatg aatgaagatg
     6421 cctcgaccta tgaggtgccg gattatatcc tgtcccgcct ccaaccgacg ttgaaagttg
     6481 aattccctaa caaagaacat gaattggcca ttttgcggta tcacctcccg tttgcatctg
     6541 cggaattaat cacgctcacg gtgaattttc ttcaaaaagc gcatcagctg gatctcccgt
     6601 tctccattcg ggacgggatg cacatggtgc aatacgcgat gaaacggatg gcccaagacc
     6661 ctcaccatcc cgtggcccgt gatccggctt ggcgggaagc cctgatcaat gttttgggag
     6721 aagaagcaca agatctggat gtgttagcga aacgccggtc ccaaacattg cacgggcatg
     6781 ctcttccgaa aggattgggg gattttttct ttgaagagga tcatcccctg caccctgacc
     6841 agtaggcctt cagacctgta acccttggtg accctgatgc acggcccatc ctccaattct
     6901 tgagtgggaa agcctcacag cttttcctcc aggacgcacc atgactctct cctcgttgat
     6961 cccagacacg atctttgccc tttcttccaa tattcacgtc ttgcctgtcg tgcatggaag
     7021 tggagatatg gctcatgtcg tccgcgagat tattgtatcc cgccacattg attgtctagt
     7081 ccttcctcta ccagcatcgg tgcaatccct catagaaaag gggattgacc agttgcctgt
     7141 tatcagtctc gtggttcttc cagaacaaca tgacgatgga accccgagct gctcttatgt
     7201 gccgattgat ccctgtcaac cggtgatcat gggaattcga tcagccatgt cggaaatgat
     7261 cccgcgagcc tttatcgatc gtgaggtccg acgctaccat cccgtctcct gggtcgggcc
     7321 tgatccctat acgttaaagg ctgtgccttt ggcagccttc tctgcggcca ccatgccttt
     7381 tttgccgcct cctcaagacc cgtcctctcg ttgggaacgc attcgttgga tggcctttcg
     7441 acttcatgaa ctcgaactgg acttttcggc cattcttttt ctctgcccaa tgaccgattg
     7501 gccctggttg cgtcaggctt atcaggagcg gatgccgtat atttcacccg agcagaacat
     7561 cagtcttccc gagtggtgga aggtcgaacc gtcttcactt tactttgcgt tgagcgaatt
     7621 accctatgtc acccatctct atgagcaacg acgggaagaa gcccgtgccg atacgcattt
     7681 ggctattgat ggcatcaaag aactcgttct ggaagcacgg gcccggtggt tggcgtctcg
     7741 ttccacaacc atcgcgcacg aaacgaattg ggtcacccca caattattgc aacgctattt
     7801 tcaatatgtg cggaatctga cacttctcga gcatcggctg aagccggatt tgtatacctt
     7861 ggttctcgct gccaagcaaa tggctggaga tgaatttgcc ctcaggttat tggaaaccgc
     7921 caaaacctat gactatcaaa ctcaaccatc cgtattggat accaggcctg gtgtgttggt
     7981 gggaattggc gaacttcaag accctggggg caacattctc ccggctgtga atcggctgca
     8041 aggagaccct ttggtctggc gccgtgttac cctccggcct gagcccccaa ggcccaaaac
     8101 acaagcttgg gcacaagaat ggaatcctta ccgccagtgt tcctggcctc cggaggatca
     8161 gcgtattgaa tcctttgcgg cccatgttcg acagcatagt cgacaggttt tgggggcaga
     8221 tttaggcagg attgaacgat tcaccaactc gctggaagat ggcatcgatc ttcgggcgac
     8281 tttgcgccag tgggctatga ctccacaacg atccgtgcgc gatattcatg tcaaagtggt
     8341 tcctcccgtg cgaggaacga ttgaatcgtt ggtgtttttc tttgaggtgc cggctgatcc
     8401 ccagaagttt tcctggcaaa cgacctggta tgccgaacat caagaagagt ccaccctcag
     8461 tttttacgcg accccctttt ccccgaacat ggtcggacca ggaatcggac aggcatgcta
     8521 tggaggcgcg cttttcctgt atccgcctcg ccttattcct gatatatggg aaaatcccct
     8581 attcgatttt gcgacgtcgc tcgaagaacg attgcttgcg ggagcctgcg cccattcaca
     8641 agaacccgtt gtggctgtcg tctctccggt tccgttgact tgggcctggc gaaagacggc
     8701 ccgtcgcttc ggccggaagc tcctgcccat tcccttacat cggttttcag ggcaaaccat
     8761 tgctcgtctg cgacaatttc atgtgttaaa tgggcacgaa atccgaagtt atgcagccaa
     8821 atttattcga gaataatcct gacgaggcag gaggagagga ttctttcaag gctcctcaat
     8881 ctcgtgctgt gccatccgca aaggtccaat cgaagcttga aaagctaaaa cacgccatcc
     8941 gtcgacatga tgaattgtat tatgttcgaa gccgaccgga aatatctgat gctgaatatg
     9001 accaactctt tcgagagctg caagacttag agagccaata cccggacctc aggacacctg
     9061 attcgccaac ccagcgggtc gggggtgcgc cacttgccca attcagtaaa attacacacg
     9121 aatttcctct tcttagtttg gactcggaaa tggatgaagc gcgcgtattg gctttcgacc
     9181 agcgggtttg ccgggaattg gacgtgcgac aggcgagcta ttcggctgaa cccaaatacg
     9241 atggattatc tgttgccctc acttacgatc atggcatatt tgtg
//
LOCUS       DATIAL010000552         4460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_422530, whole genome shotgun sequence.
ACCESSION   DATIAL010000552 DATIAL010000000
VERSION     DATIAL010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422530"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VLA60_16910"
     CDS             <1..1173
                     /locus_tag="VLA60_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11095.1"
                     /translation="MMCVWRWVGVVLFLSILSCQMREVKPTFKDECGILRSNVDWSNR
                     IKVLNLLSLAFSQKCDAAVIEYTEEAQSGFRDKTFSFSREMAGVFLSDGVLTDYVLES
                     YERAYLSVLLAASYLRTGHVEDAKVELRRLDHELFAPLYNFGEDPVNLMLSAVLWEVL
                     GEPGDARTDWFRLAEPTSSFLLHVPQALQSFAQKQVIRLDQGARPIPRWEVYGIGRFP
                     EVDWDFKLFGAPNGYFVISPKPPFQELCVSETGLRLSTESWFAKIAHRHDHAYHPLLN
                     IQSWIRLPVGVVYGLVPFSLGVGVAVGGCAGAASLGGRGSGDLCALSVLGGTQLMQVA
                     PTVFQNTVRPDLRHWELLPAAIVVTQESDVGSEPCDSHQARLHHLVTRASAPPLSS"
     gene            1541..1981
                     /locus_tag="VLA60_16915"
     CDS             1541..1981
                     /locus_tag="VLA60_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11096.1"
                     /translation="MTYTPLEDARLFPFRQAGLIGSLRLGLTLLAMGVMMMGGSKGCA
                     EAKPPETLAPAPQSIAVYALSRGKGVPDQAIEVLAHSRTLLKSAQERGEVRRLVEQRI
                     GIEGETRLCAEFSDVESAHSIIEQIQQLGEGVDLINVKLEPCPP"
     gene            2092..3405
                     /locus_tag="VLA60_16920"
     CDS             2092..3405
                     /locus_tag="VLA60_16920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HSF11097.1"
                     /translation="MKNCRFFLNRALTVVGMVIGLTACSQLHTTPLPAVDSGPKNSPE
                     QGKQILSDNAKQKRDEWKTKTFEEFKAQTYKEPFEGGKYIVNGDTPILNEKLLQEFFE
                     TRIKESINSREGVRTKLTVHQVNGQDAVWNSIEKRQLTYCVSKSFGVNYEKMKSDMEA
                     AGNAWEAVAAVDFIYVGGEDDSCTASNPNVVFDVRPVNVNGQYLARAFFPNEQRSSRN
                     VLVDNSSFQLDPNGKLSLQGILRHELGHTIGFRHEHTRPDSGTCFEDSNWRPLTSYDA
                     FSVMHYPQCNGKGDWTLTLTNIDKNGAACLYGPAQGFTIDTAICQGPDEPPGPIACGP
                     KTETFVGQSVAKNAEKTYGPFMVVPGTLVEVVMHGEANSGDPDLYVRFNQDPTTTAYD
                     CRPYLSGAEEKCVLDVPTNGTAVHVKVRGYSDAHFNLTVTHTPTH"
     gene            complement(3522..>4460)
                     /locus_tag="VLA60_16925"
     CDS             complement(3522..>4460)
                     /locus_tag="VLA60_16925"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF11098.1"
                     /translation="TTRNMLHIFFLPFTAVRLSHYHLLTAILAIGLLLGFSCLAIAET
                     HLPPDEVDEHIAHIASLAKPSPQVAIPEGVFLMGTNRRDDLRHSLEMHYDDTEFPQRH
                     IWVDRFLMDRYEGTLSEYLAFLLTTNRPVSQELRGLIWHLISVHFIPDQALASWPALY
                     ITWEEAKAFCEHHNKRLPSEAEWEKAARGESARIFPWGEMDPTGDIAVFGQYHVHEIP
                     LVATVDSFEDGQSIYKILHLAGNIAEWVNDWLGPDYYPIMPARNPPGPKSGRYKVVRG
                     GSWKSRPVMLRAATRGGAIPEERSASIGFRCAQSSP"
BASE COUNT         1087 a          994 c         1182 g         1197 t
ORIGIN      
        1 atgatgtgtg tgtggagatg ggtgggggtg gtgctctttc tttcaatttt aagttgtcag
       61 atgcgagagg tgaaaccaac atttaaggat gaatgtggaa ttcttcgttc caacgttgat
      121 tggtccaatc ggattaaggt gttgaatctc ttaagcctgg ccttttccca aaaatgcgat
      181 gccgcagtca ttgaatatac ggaagaagcc caatctggat ttcgcgataa aactttttct
      241 ttctctcgtg aaatggccgg ggtcttttta tctgatgggg tgcttacgga ttatgtgttg
      301 gaatcgtatg agcgggcata tttaagcgtt ttgcttgccg cgagttatct gaggaccgga
      361 catgtcgagg atgcaaaagt ggaattacgg cggttggacc atgaactgtt cgccccgttg
      421 tacaattttg gtgaagaccc tgtcaatctt atgctttccg ccgtgctatg ggaagtcctt
      481 ggtgaaccag gagatgccag aaccgattgg ttcaggttgg cggagcccac cagttctttt
      541 cttctgcatg tgcctcaagc tcttcaatcg tttgcccaaa agcaggtgat caggctggat
      601 cagggtgctc gtcctatccc ccggtgggag gtctatggga ttggtcgctt tcctgaggtc
      661 gattgggatt tcaaattgtt tggtgccccc aatggatact ttgtgatttc tcccaagccc
      721 ccttttcagg agttgtgtgt ttctgaaacg ggcctacgtc tttcaacgga aagttggttt
      781 gcaaaaatcg cgcacaggca tgatcatgct taccaccctc ttttgaacat tcagagttgg
      841 attcgtttgc ctgtcggcgt ggtttatgga ttagtgccct tttctcttgg ggtcggtgtg
      901 gctgttggtg gatgtgcggg ggcagcgtca cttggaggaa gaggcagcgg tgatttgtgt
      961 gccctgtccg ttctgggtgg cacgcagtta atgcaagttg ctcccacagt gtttcagaat
     1021 accgtacgtc cggatttacg tcattgggaa ctgctccctg cagcgattgt tgtcactcag
     1081 gagtccgatg tgggctctga gccctgtgat agtcatcagg cgaggttgca ccatttggtg
     1141 actcgtgctt ctgcccctcc tctaagttct tagaaatagg gattttcttc agaaccattt
     1201 cattcggacg ggattgcctc cttccgggtt agtggtccat gccagtccaa atgaaatgga
     1261 ttagggcgat atttatttaa ttggccatga ggcttgcgga aattttgaga gccaatggtc
     1321 caaagggata tcgcccctga atgaaatggt tactaaggat tttatccatt tagatacaat
     1381 gtgtatcgaa aaaggagaat gtatgaatag ccaaggataa ggtgacagat cggcaaagaa
     1441 gcatacgcgt tggatattcc cgagattttt tcttcacccc ccgtgtatgt catactgcat
     1501 tatcatggga ataggtcttg ctttatgaaa ggaatacctt atgacataca caccattgga
     1561 agatgctcga ctgtttcctt ttaggcaagc cgggttaatt gggtctcttc gacttggcct
     1621 gaccttgttg gcaatggggg tcatgatgat ggggggcagc aaggggtgtg cagaggccaa
     1681 gcctccggaa accctcgctc ctgctccaca aagtatagcg gtttatgctc tttcgcgtgg
     1741 gaaaggtgta cctgatcagg ccatcgaggt actagcccac tctcgcaccc ttctcaagtc
     1801 tgcccaggaa cgcggggagg tccggcgatt ggttgagcaa cgtattggaa tagaagggga
     1861 gacgaggctc tgcgcggaat tttctgatgt ggaatcggct cattcaataa ttgagcagat
     1921 tcagcaactc ggagagggag tggatttgat caacgtaaaa cttgagccgt gtcctccatg
     1981 atcaacttct attctccacg aattcttttg gggtgacgcc atctagtggt tcccctggtg
     2041 ctcttgttga tcagcaggga ggttcatcat tactttcaaa ggagtggaat tatgaaaaat
     2101 tgccgatttt ttctcaatcg ggcattgacg gtggtaggaa tggtcatcgg attgactgcc
     2161 tgttcccaac tccatacaac tccactacca gctgttgaca gtgggcccaa aaattctccg
     2221 gaacagggca agcaaatttt gtcggataac gcaaaacaga aacgagatga gtggaaaact
     2281 aaaacctttg aggagtttaa agcccaaacg tataaggaac catttgaagg tgggaaatat
     2341 attgtgaacg gtgatacccc cattctcaat gaaaaactcc tacaggaatt ttttgaaacc
     2401 cgaataaagg agtcgatcaa ttctcgggag ggggttcgaa cgaaattaac cgttcatcaa
     2461 gtcaatggtc aggacgcagt gtggaattct atagaaaaac ggcaactgac ctactgcgtt
     2521 agtaaatcat ttggcgtgaa ctatgaaaaa atgaagtccg acatggaggc cgccggaaat
     2581 gcatgggaag ctgtggctgc tgttgatttc atttatgtgg ggggagagga tgattcttgt
     2641 acggcgtcca acccgaatgt ggtttttgat gtacgcccgg tcaacgtgaa tggccaatat
     2701 ctggctcgag cctttttccc gaatgaacaa cggtcctcac ggaatgtctt ggtggataac
     2761 agttcttttc aattggatcc aaatggcaag ctatcgttac aagggatatt gcgacatgag
     2821 ttaggtcata cgattggatt ccggcatgaa catacccgcc cggattcggg tacgtgtttc
     2881 gaagatagta attggcggcc gttaaccagt tacgatgcct tctcggtcat gcattatccc
     2941 caatgtaacg gtaaagggga ttggaccctg accctgacca acatcgataa aaatggggca
     3001 gcgtgtttgt atggccctgc gcagggattc accatcgata ccgcaatttg tcagggaccc
     3061 gacgaaccgc cgggaccgat agcctgcgga cccaaaacgg aaacttttgt cggacaaagt
     3121 gttgccaaga atgcggagaa aacctatggc ccctttatgg tggttccagg aacattagtg
     3181 gaagtggtta tgcatggaga agctaattcc ggtgatcccg atctctatgt tcggttcaac
     3241 caggatccga ccacgactgc ctatgattgc cgtccttatc tctcgggtgc ggaagaaaag
     3301 tgcgtccttg atgtgccaac caatgggact gcggtccatg ttaaagttcg gggatattct
     3361 gacgcccatt tcaatctaac tgtgactcat actcctacac attaatagct cagcacatct
     3421 gatatcaggg cagagaggct tttgcctctc tgccctgatc cagttgaaac gctggcgtga
     3481 ggacaggttc ctgaaaagtt gttgatgaga gattaactga gttaggggga ggattgagca
     3541 cagcggaagc caatgctcgc ggaacgttct tctggaatgg cccctccacg agtcgctgcc
     3601 cgcaacatga ccggccgact tttccatgat ccgccccgta caaccttata acgccccgat
     3661 ttgggaccag gcggattcct tgccggcatg atggggtaat aatctggtcc cagccaatca
     3721 ttaacccatt ccgcgatatt accagccaga tgcaatattt tataaatact ttggccatcc
     3781 tcgaagctat ccaccgttgc aaccaaagga atttcatgga cgtggtattg tccgaaaacg
     3841 gctatgtctc ctgtcgggtc catttccccc cacggaaaga tgcgggcaga ctcccctctt
     3901 gccgccttct cccattcagc ttccgacggc agtctcttgt tatgatgttc gcaaaatgct
     3961 ttggcttcct cccacgtgat gtagagggcc ggccaggaag cgagtgcctg atcagggatg
     4021 aaatgcacac taataagatg ccaaatgagt ccccgtaatt cctgagagac cggcctgttg
     4081 gttgtgagca gaaatgccag atattcgctt aaggtgcctt catagcgatc catgagaaat
     4141 ctatccaccc aaatatgacg ttgaggaaat tcggtgtcgt cataatgcat ttcaaggcta
     4201 tgccgaagat cgtcgcgacg attcgtcccc atcaaaaata ctccttcggg aatggcaact
     4261 tgtggggatg gtttggccaa gctggcgata tgagcgatat gctcatcgac ttcgtcggga
     4321 ggtaggtggg tctctgcgat tgccaaacag gaaaatccca agagaagccc tatggcaagg
     4381 atggcagtca acaaatgata atgcgatagt cgaactgctg tgaacggcag gaagaatatg
     4441 tgaagcatgt ttctagttgt
//
LOCUS       DATIAL010000553         3766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_206228, whole genome shotgun sequence.
ACCESSION   DATIAL010000553 DATIAL010000000
VERSION     DATIAL010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206228"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VLA60_16930"
     CDS             complement(<1..1294)
                     /locus_tag="VLA60_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HSF11099.1"
                     /translation="MNDRDSPLRTYIQNHRVTFEDLLGQMVEVPSVSSDPAHASDMHR
                     MAELAVQYLEHFGAKASIVKTSGYPSVSGEWFAGRQFPSLTIYNHLDVQPAQEPEWRQ
                     PPFTFYKDGERYCGRGATDDKGPALTALFAAKFAREAGIPLNIRVLWELEEEIGSPHF
                     AEVLQSRKTVPPSDSVLVSDTIWVAKNRPALSCGLRGLLAARLVLRTGETDIHSGLTG
                     GGARNPLAELCDAAYSCLDAKTGRVKISGFYDDVVGPTQTEMKNFVASGFQVRLFKEA
                     YQLRSLRTSDPKDLLRRIWASPTFEVHGLTGGHMGPGVKTIVPAWGELKVSMRLVPNQ
                     HPQKIFRLLKKHLRARNPDIEVINEGELEPYRGITEGPYAESLQKAVTLGFGRRPVFV
                     REGGSIGAIAVLQRAWKVPILFMGLSLPEHGYHAPNEHF"
     gene            complement(1409..2539)
                     /locus_tag="VLA60_16935"
     CDS             complement(1409..2539)
                     /locus_tag="VLA60_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HSF11100.1"
                     /translation="MGHSPHEGHPLPDEQPPETPPDQHAPEPFMPASSDYFSSPSLLT
                     PFPKDNNIHERMIANPDRTDPIYALPPEEYVEEDQEEILYQEPQPPSTFSQWGLPILL
                     FGLTVFTTLWAGAFQVNTKPVNGAWDFLIRYPGSLIQGIPFAATLLGILVIHESGHYV
                     LSRIHRVPASFPLFIPGPPHFIGTFGAVIRMRSAIMNRRALFDIGVAGPIAGFVAAVV
                     AILVGLSLSDLVPRSSTFGLQLGEPLLLQFFAWLIFGPIPPTHDIVLHPIAFAAWFGF
                     FVTAINLLPLGQLDGGHVAFAVLGRRQRTLALATIPVLIYLGLTGWPGWILWVALAGL
                     VGIAHPPVVDPHTSLGERRRWVAWTALAIFIVTFAPVPFSVE"
     gene            complement(2589..3440)
                     /locus_tag="VLA60_16940"
     CDS             complement(2589..3440)
                     /locus_tag="VLA60_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HSF11101.1"
                     /translation="MNLEDEFCDIMKKARFGQGLGIETLAELAKVEQADVERLEKGHR
                     LPTKGEIQGISQALHLRVGPLVSLTLEGWLPQPQPVWTTEHGLVQTIKGDIGGYEVKG
                     YLLTDPATKQVLMIDTGYNAKQMLATIAQRELTLIGLCLTHGHADHAGGLEEILAQWP
                     VPVYLGNGDFDLLPWKPSDASVKIPADHQMIALGELTIECLATPGHTPGGFCYLLSFK
                     GHTLCFVGDTLFAGSVGRSNPFSLYPLHLQSVHQTVLRLPKETILFPGHGPSTTVAEE
                     QAHNPFG"
BASE COUNT         1010 a          928 c          984 g          844 t
ORIGIN      
        1 cgaaatgttc attcggagcg tggtacccat gttccggtag actcagaccc ataaacagaa
       61 tgggaacttt ccaggcgcgt tgtaatacgg caatggctcc gatagacccg ccttcccgca
      121 caaacaccgg ccgacgtcca aatccgagtg tcacggcttt ttgcagcgat tccgcataag
      181 ggccctcggt aatcccacga tagggttcca gctctccctc gttgatcact tcaatatcgg
      241 gattgcgtgc acggaggtgt tttttcaaca gtcggaaaat tttttgggga tgttggttgg
      301 gaaccaagcg catgctgact ttgagttcac cccaggcggg aacaatggtt ttcactccag
      361 gccccatatg tccaccggtt aatccatgta cctcaaaagt aggcgaggcc cagatccgtc
      421 tcagtaaatc tttcggatcg gaagttcgaa gcgatcgcag ttgataagct tctttaaaga
      481 ggcgaacctg aaatcctgag gccacaaaat ttttcatttc cgtctgcgtc ggccccacga
      541 catcatcgta gaaacccgag atcttcaccc gcccggtttt cgcatccagg caggagtagg
      601 cggcgtcaca caattcggct aaaggattgc gcgctcctcc tcccgtgaga cccgaatgaa
      661 tatcggtttc cccagttcga agcaccaagc gggcggctaa taacccacga agaccacagg
      721 agagagccgg tcggttcttt gccacccaaa tcgtatcgga caccagcact gaatcggagg
      781 gagggactgt tttacggctt tgaagcacct cggcaaaatg agggctgcca atttcttctt
      841 ctaattccca aaggacccga atgttgaggg gaataccggc ttctctcgca aatttggcag
      901 caaagagagc ggttaacgcg ggtcctttat catccgtggc accacggcca cagtaccgtt
      961 cgccatcttt ataaaaagtg aaggggggtt gacgccattc cggttcctga gccggttgga
     1021 cgtctaaatg attgtaaatg gtgagactgg gaaattgtcg tccagcgaac cattccccgg
     1081 aaacgctggg atacccactg gtcttcacaa tggatgcctt tgctccgaaa tgttccagat
     1141 actgaactgc taattccgcc attctgtgca tatcggaggc atgagcaggg tctgaactga
     1201 ctgaaggcac ttccaccatc tgccccaaca gatcttcaaa ggtgacacgg tgattctgaa
     1261 tataggtgcg taaaggggaa tcacggtcat tcatgagata acggttcctt tttgtgaatg
     1321 aatccgatga gaaataaggg attggtagtc gggaacgaag ggcgtatccc atgaacaatg
     1381 acacgaatgg acaagcctag catgtccttt actcaacgga aaacggcacg ggagcgaagg
     1441 tcacaataaa aatggccaag gcagtccagg cgacccatcg gcgacgctca cctaaacttg
     1501 tgtgcggatc aaccaccggc ggatgcgcga tgcccacaag tccagccaga gcgacccaga
     1561 gaatccatcc gggccatcct gtaagaccca gataaataag gacgggaata gtggccagtg
     1621 cgagtgtccg ttgacggcgg cctaagaccg caaaggctac atggcctccg tccaattgtc
     1681 ccaagggtaa caaattaata gcagtgacga agaatccgaa ccatgcggcg aaagcgatgg
     1741 gatgtagcac aatgtcgtgc gtgggtggaa tgggaccaaa tatcaaccag gcaaaaaatt
     1801 gcaaaagaag aggttcgccc aactgcaggc caaaagtcga ggatcgtgga acgagatcgg
     1861 agagggacag ccccacaaga atagccacga ccgcagccac aaaaccggct atgggaccgg
     1921 ccacgccgat atcgaagagc gcacgacgat tcatgatggc cgatcgcatg cgaatcacgg
     1981 ccccgaaggt gccgataaag tgtggaggtc cgggaatgaa aaggggaaac gaagcgggta
     2041 cccgatgaat gcgggagagc acgtaatgcc cggattcatg aatgacgaga atgcctaata
     2101 aagtggccgc aaagggaata ccctgaatca acgatcccgg ataccgaatt aaaaaatccc
     2161 aggctccatt aaccggtttc gtattaactt gaaaggctcc cgcccagagt gtggtgaaca
     2221 cggtgagccc aaaaagcaaa ataggaaggc cccattgaga aaacgtactg ggcggctgtg
     2281 gttcctgata taaaatctcc tcctggtcct cctccacata ctcctcggga gggagggcat
     2341 atatcggatc ggtccgatca ggattcgcga tcatgcgttc gtgaatgtta ttgtctttgg
     2401 ggaatggcgt gagaagggat ggggaagaga aatagtcaga agaagccggc ataaatggct
     2461 cgggtgcgtg ctggtcgggt ggcgtttccg gtggttgctc atccggaaga ggatgccctt
     2521 cgtgaggtga gtgacccata gttgtctatt tcagaaacag gcgaaaattg ttcaacaaat
     2581 tctccctctt atccaaaggg attgtgtgct tgttcttcag ccaccgtggt ggaaggacca
     2641 tggcccggga ataagatagt ctccttcggt aaccgtaata cggtttggtg aacggattgt
     2701 aaatgtagag gatatagcga aaagggattg gaacggccca ccgaccccgc aaacagggta
     2761 tcgcccacaa aacacagggt atgaccttta aaagataaaa gatagcaaaa tcctcccggc
     2821 gtatgacccg gtgtggccaa acattcaatc gtcaattctc ccagggcgat catctggtgg
     2881 tcggccggga ttttgacgga agcatcggat ggtttccatg ggagtaagtc aaaatctccg
     2941 ttccccaaat agacaggaac tggccattgg gccagaattt cttcgaggcc tccagcatga
     3001 tcggcatgtc catgcgtgag acagaggcca atcaaagtca attcacgttg tgcgatcgtc
     3061 gccagcattt gcttggcgtt ataccctgta tcgatcataa gtacctgttt ggttgcggga
     3121 tcagtcaata aatacccctt gacttcgtac ccgccaatat cgcctttgat ggtctggacc
     3181 aggccatgtt cagtcgtcca gacgggttga ggttggggca gccatccttc cagggtcaga
     3241 cttaccagag ggccaacgcg gagatgcaag gcctgactga tcccttgaat ttcccctttg
     3301 gttgggagcc ggtggccttt ttcaagccgc tccacatcgg cctgctcgac tttcgccaat
     3361 tccgccaggg tctcaattcc aagtccctgc ccaaagcggg cctttttcat gatatcgcaa
     3421 aattcatctt ctaagttcat aaaaaacctg caaaataaaa caatctttga tctgatatag
     3481 cattgaagat tgcaagatta attcgtgtct tttaccagat ggtcatttca agggagtttc
     3541 tccatgcttc tcccattatg ggtatgagat ccatatcaag caagaccacg cttatgaata
     3601 aggttgggaa aaatcaaatt tccctctaaa aattcgttgg aaccaccatg ggaaacaaac
     3661 tacttacgtg attgatggcc tcatcaattt aagtcatgat gaaattaggt atttaaatga
     3721 atctttgttg ggattcatgg cgggcaaacc tattccgaga gcggaa
//
LOCUS       DATIAL010000554         4852 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1604560, whole genome shotgun sequence.
ACCESSION   DATIAL010000554 DATIAL010000000
VERSION     DATIAL010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604560"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VLA60_16945"
     CDS             <1..1177
                     /locus_tag="VLA60_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HSF11102.1"
                     /translation="TDEPFSASDQDRWAYAGGIRLDYSHRLNPDHLIKTGFQIQRTQA
                     VNKTRLSAFLRDGAGDPIGGVISRNADNRTIGWREEFWIQDQWTPWAQWTFNLGVRFD
                     QIQALTNDGQVSPRVGATYAMTPNHVFHAFYGRLFTPPNLESVPFLQLNTIGTTAQPE
                     NLTNRTVEPERAHYFEVGSAHALGDSLSIELTGYYKLNKNLSDAGQFGTSPLLNYFAF
                     KRGWQRGIDLTVKAKFLENLYGRGNVAWGQCRGKKLQSGYFLLEQNEINDINSNSGVY
                     CDHMQLVTSSAVLTYRPFAHTTFTGQMLFGSGLRSSNPGGKTNATHSPSYTTYNLSIE
                     QLLPLWDNYKALLGFDVINLLDQHVFLNNGEGSIGLGVAHANMPRSFFFRGQFFFGG"
     gene            1182..1628
                     /locus_tag="VLA60_16950"
     CDS             1182..1628
                     /locus_tag="VLA60_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HSF11103.1"
                     /translation="MNVFRNLGFFMQVLWLLNGPSLSYPAESDFATHQSDHEEDVQVE
                     TLDTVHVHGLRLNKDQQIGPVPQRTPWPTIPSTLDGQILDDWLKARILVSKNSDVTVV
                     ILQPAAHRELTQASLLALKQWTFLPQMNGEEPIDGEVSLRIHFRTQ"
     gene            1987..>4852
                     /locus_tag="VLA60_16955"
     CDS             1987..>4852
                     /locus_tag="VLA60_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF11104.1"
                     /translation="MFLMVTALLLSLSVFVFIGLYFEAVEDEKDRLTHLTSIYVHYLE
                     AMNQLTPGWDEGEMSQEFFANRIQQEFDSLRDQHHFGQTGEILLVRRHENGGQSSSQF
                     QLDLSVDSRTQILPDSHGNSVLLAYQPLAGFPLGVIAKINIQEIQIPYFWGGGSIGLL
                     EVGIMIVVGFILVQGKNARRQHSPKDRQKIRGVIASECEGIVTFNENGKIETFNEAAE
                     AMFSWQASEVIGRPFRNLVISPEQDVWDLFVLSYRQSRVKHIGGLLKEFKAQKKDGTV
                     FPLSLTLTESALAGQRLFTALLRDETEQKLAERRLAAQYAIARVLADCTTIQEATPEI
                     LQAVCNSLGWQVGVLWQVKPGTKILFCVEVWRTSHERFSEFVTVTQSMSFSKGIGLPG
                     RIWETGNPIWIPDAVKDANFPRAPMAAQVGLHAAFAFPIRLGETILGVMEFFSREIQE
                     PDEALLHQMVSVGSQIGQFMQRKETEMALRESEERTRLILDTALDAVVTLDEHGYVIG
                     WNSQAERMFGWSREEIIGQQVLTTIIPPQYRDAYPQGIQHYLTSEVSAILNKRIELSG
                     LHRDGHEFPLELAITAVQSQGKTIVSGFLRDLTERRQTEEALLKSEEQRRQAQKMEAV
                     GTLAGGIAHDFNNILSAIIGYTELAMTQIGVGSPVLPRLQEVLRASYRAKNLVRQILT
                     FSRRDESGRKVIHLQPIVQEALNLLRASLPSTISLHAELQPSSAPVLADATQIHQVVM
                     NLGANAEYAMRPMGGRLTVTLDEVANDETTASLVQGLHAGPYIRLRVADSGQGMSPSV
                     TKRIFDPFFTTKDVGEGTGMGLAVIHGVVTSHGGVIRVDSQKGQGTTFTIYFPCSSLP
                     VEVRQVPSKSSSYCRKGTVMFVDDEVSIAKWAKDMLESMGYMVVVFTNGPEALQAFKE
                     NPDQFDVLVTDQTMPGMTGELLARQVMSIRPGFPVILCSGF"
BASE COUNT         1224 a         1055 c         1236 g         1337 t
ORIGIN      
        1 tactgacgag cctttttcgg caagcgatca ggaccggtgg gcctatgctg gagggatacg
       61 gttggattac agtcatcgat taaatcccga tcatttaatt aaaacgggat tccagattca
      121 aaggacccag gctgtcaata aaacccggct gtcggctttt ttgagggatg gagcgggcga
      181 tccgataggg ggagtcattt ctcgcaatgc cgataaccgc accattggat ggcgcgagga
      241 attctggatt caagaccaat ggaccccctg ggctcaatgg acttttaacc ttggcgtccg
      301 cttcgatcaa atccaggcgt tgaccaatga tggacaagta agtccgcggg taggcgccac
      361 gtatgccatg actcccaatc atgtctttca tgccttttat ggaagattat ttactcctcc
      421 caatcttgaa tccgttccat tccttcagtt gaatactatt ggtactaccg cacaaccaga
      481 aaatttgacg aatcgaaccg ttgaacctga gcgagcgcat tatttcgaag tcggctccgc
      541 ccatgccttg ggggattcat tgtccataga attaaccggt tattataaat tgaataaaaa
      601 tctatcagat gcgggccaat tcggaacctc cccgcttctt aattattttg cgtttaaacg
      661 tgggtggcag cggggaatcg acctgacggt taaggctaaa tttttagaaa acctctacgg
      721 gcgtggtaat gtcgcttggg gtcagtgtcg cgggaaaaaa ctgcagtcag gttatttttt
      781 gttggagcaa aacgaaatta atgatataaa ttccaatagc ggagtatatt gtgatcatat
      841 gcaattggtc acgagttcgg ctgtgcttac ctatcgccct tttgcacata cgacgttcac
      901 cggacaaatg ttgtttggat ctggactccg gtcatcgaat cctgggggga aaaccaacgc
      961 gacccatagc ccgtcgtaca caacctataa tctttccatc gaacaacttc ttcctctttg
     1021 ggataactat aaagcgctgt tggggtttga tgtcatcaat ctcctggacc aacacgtgtt
     1081 tctaaacaat ggggagggca gtattggcct cggcgtagct catgccaaca tgccccgttc
     1141 atttttcttt cgtggacaat tcttttttgg tgggtaatgc tatgaacgtt ttccgaaatc
     1201 ttggtttctt catgcaagtg ctctggctat taaatggtcc gtcgctttcc tatccggccg
     1261 aatcagattt cgcgacgcat caatctgacc atgaagaaga cgttcaggtg gaaaccctcg
     1321 atacggtcca tgtccatggg ctacgactca ataaagatca acaaattggg ccggtcccac
     1381 aacgaacacc gtggccgaca attccatcta ccttagatgg gcagatctta gatgattggc
     1441 tcaaagctcg aatattggtc agtaaaaatt cagatgtgac cgtggttatt cttcaacctg
     1501 ccgcacatcg ggaactcact caagcttctt tactggctct caagcaatgg acgtttttgc
     1561 ctcaaatgaa tggcgaggaa cctatcgatg gtgaggtctc gctccgtatt cactttcgta
     1621 ctcagtaaac atttctgacc cgtataggtg ctaaacgggt ggcagaacag tttcctcaag
     1681 atgtttaggt cttaatgtcc aatggggttg tagaaccctg aggagggctt ttgtctttct
     1741 tccgcaacta tccttccatg ttggtgttcc ggatagacat tttttgtgca aaagcatagg
     1801 atattccctt gactttgtgg atggttggtg gcttccattc gattgaggga ggggatcatt
     1861 gattctcttt ggcattcttc ctcgtgcttt cacgcatggt tcctttagct ctgctgttcg
     1921 tcctgccttt cttttctgtt ccattcccgg tgcttgatgc ttcctgatca gcaaagaatc
     1981 ctaaaaatgt ttctgatggt gactgcattg ctcctgtccc ttagcgtttt tgtgtttatt
     2041 gggctttatt ttgaagccgt cgaggatgaa aaagaccgac tgactcatct gaccagcatc
     2101 tacgttcatt acttggaagc catgaatcaa ttgactcctg ggtgggacga aggggaaatg
     2161 tctcaagagt tcttcgcaaa tcgcattcaa caggaatttg attctctgag agatcaacat
     2221 cactttgggc aaacaggaga gatacttcta gttcgccggc atgaaaatgg agggcagagt
     2281 tcttctcaat ttcaactgga tttatccgtc gatagccgga cgcaaatttt gcctgatagt
     2341 catggtaata gcgtcctact agcctatcaa cccctagcag gttttccttt gggagtaata
     2401 gcaaaaatca atattcagga aatccaaatt ccctattttt ggggtggagg atccattgga
     2461 ttactggaag taggaattat gatagtagtc ggatttatcc tggttcaagg taaaaatgcc
     2521 aggaggcagc attcgcctaa agatcgacaa aaaatccgag gtgtcatagc gagtgagtgc
     2581 gagggaattg tgaccttcaa tgaaaatggg aagatcgaaa catttaatga agcagcggaa
     2641 gcaatgtttt cctggcaagc ctccgaggta ataggtaggc cgtttagaaa tctggttatt
     2701 tccccggaac aagacgtgtg ggacctattt gtcttgtctt atcggcaatc gagagtaaaa
     2761 catattggtg gtttgctgaa agaattcaag gctcaaaaaa aagatggaac ggttttccct
     2821 ctgtcgttga ccttgaccga gtcggcgcta gcgggccagc gcctcttcac agcgttatta
     2881 cgggatgaga cagaacaaaa actagctgaa cgccgtttgg cggcacaata tgcgatcgcc
     2941 cgggtgttgg ccgattgcac gaccattcaa gaagccactc cggaaattct ccaggcggtc
     3001 tgtaatagtt taggatggca agtgggtgta ctctggcaag tgaagcctgg gacaaaaatc
     3061 cttttttgtg tggaagtgtg gcgaacctcc catgaacggt tttctgaatt tgttactgtg
     3121 acgcaaagta tgtccttctc aaagggaatt ggcctgcctg gacggatctg ggagacaggt
     3181 aacccgatat ggattcctga tgcggtcaag gacgccaatt ttcctcgtgc accaatggcc
     3241 gctcaggtag ggttacatgc ggcgtttgcc tttcctatca ggctaggtga gacaattctt
     3301 ggagtaatgg agttttttag ccgggaaatc caggagccgg atgaagcctt gttgcaccaa
     3361 atggtgtctg tcggaagcca gattgggcag tttatgcaac gaaaagagac agagatggcc
     3421 ctccgggaaa gtgaagaacg aacgcggctc attctggaca cagcccttga tgcggtggtc
     3481 accctcgatg aacatggcta tgttatcggg tggaactcac aggcggaacg catgtttgga
     3541 tggtccaggg aggaaatcat tgggcaacaa gtcctaacta ctataatccc gccgcaatac
     3601 cgggatgcct atccacaggg aattcaacac tatctcactt cagaggtcag tgcaatttta
     3661 aataaacgga ttgaattgtc cggattacat cgtgatggcc atgagtttcc tctggaattg
     3721 gcgattacgg ctgttcaatc acaagggaaa acaattgtta gtgggtttct ccgcgattta
     3781 accgagcgac ggcagacaga agaagccctg ttgaaaagtg aagagcaacg tcgtcaagcc
     3841 caaaaaatgg aggcggtagg gaccttggcc ggtgggattg cacatgattt taataatatc
     3901 ttatcggcca taattggcta taccgaactg gccatgactc aaattggggt gggaagtccg
     3961 gtgttgccac gtttacaaga agtcttacga gcaagttatc gcgccaaaaa tcttgttcgg
     4021 caaatcttaa ctttcagccg gcgagatgaa tctgggagaa aagtgattca tttacagccg
     4081 attgtgcagg aagcgttgaa cctccttcgt gcttctcttc cgtctacgat ttccctacat
     4141 gccgaactcc aaccaagctc tgcgccggtt ctggctgatg caactcagat ccatcaagtg
     4201 gtcatgaatt tgggggctaa tgcagaatat gcgatgcggc cgatgggcgg aaggttgacg
     4261 gtcacattag atgaagtagc taatgatgaa acgacggctt ctcttgttca agggctgcat
     4321 gcggggcctt atatccgcct gagagttgct gattccggac aagggatgtc accgagtgtt
     4381 acgaaacgaa tttttgatcc gttttttacc accaaggatg ttggggaagg cacagggatg
     4441 gggttggccg ttattcatgg ggtcgtgacg agtcatggag gtgtgattcg agttgatagt
     4501 cagaaaggcc agggcacgac ctttactatt tatttccctt gttcctctct cccagtcgaa
     4561 gttcggcagg ttccgtcaaa gtcttcttct tattgtagga agggaacagt gatgtttgtg
     4621 gatgatgagg tgtctattgc caaatgggcc aaggatatgt tggagagtat gggctacatg
     4681 gttgtcgtat tcaccaatgg tcctgaagca ttgcaggcat ttaaagagaa tcctgatcaa
     4741 tttgatgtac ttgtgacgga tcaaaccatg ccaggcatga cgggagaatt gctggctcgt
     4801 caagtaatga gcatacgtcc aggtttcccc gttattttgt gttcagggtt ta
//
LOCUS       DATIAL010000555         6277 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_423078, whole genome shotgun sequence.
ACCESSION   DATIAL010000555 DATIAL010000000
VERSION     DATIAL010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423078"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VLA60_16960"
     CDS             <1..288
                     /locus_tag="VLA60_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HSF11105.1"
                     /translation="PEAKFVLFEAFDEYTTNVTLKVCDDDDVLLATHWNGRPLTRDHG
                     GPVRVIIPKRYGWKGAKWVKKITFSDRDQKGFWEVRGYSNTALPWDNDRYG"
     gene            354..1466
                     /locus_tag="VLA60_16965"
     CDS             354..1466
                     /locus_tag="VLA60_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HSF11106.1"
                     /translation="MSVRDPFCITKVEAWSIALPLLAPFVVASGAMTVAHNVFVRVTL
                     RNRAYGFGEMAPFPEISGEDQAGSLRSFPLAAKACLGQPVTHYRKLAHRLREVAWENP
                     SVRCGIETAFLDALCRGMGIPLWGLWGGADVRPREIDVTLPIGPLDQVVATARSWYAR
                     EFRIFKMKIGLEVDEDIKRIVAVCAACPHSKFILDANEGFSLEQASECLKTMERLRLP
                     VILFEQPVAREDVEGFVTLRRRGTIPLAADESVRSVQDARRLIERNAVDVMNLKITKC
                     GVVESMDIAGLARASGLRLMIGGMVESRIAMGCSFSLALGLGGFDFLDLDMPLLLSID
                     PVQGGYSYKGPVLQPWEESGLGMEMSQEPSSMIVVE"
     gene            1754..3313
                     /locus_tag="VLA60_16970"
     CDS             1754..3313
                     /locus_tag="VLA60_16970"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSF11107.1"
                     /translation="MGTGNRLMGWSKSLGLVGLVWCVCAGTVFGQDLASFEQRVTRHT
                     LKNGWTFIIVERPVAPVFAFMTRVNVGSAQEIMGQTGLAHMFEHMAFKGTPHIGTTDY
                     DAEKVALEALEEAYQAYQTEKFAADPDPEKVDRLYAVFKERQQQASSYVVKNEFGDII
                     EREGGVALNAFTGADVTGYFYALPANKVELFAYLESERFLHPVFREFYEERDVVMEER
                     RMRTESQPFGRLLEQFVATAYMAHPYHHPVIGFASDIQSYTMTDAKKFYEEQYVPTNL
                     VTAIVGDVKAEAVIPILEKYFNRIPASPAPPPLRIVEPPSIAEKIVKLQDPSQPLYVE
                     GYHKPPVTHADQPVYDAIDDILTNGRTSRLYRSMIRDKRIAVSVGAYGEYPGDKYPHV
                     WMAYAVPGRGVTNETVQQALREEFDRLKTQDVSDEELARFRTRAKAGLVRALNNNLGL
                     AMHLTDYQMLFGDWRELFRSIDRLDQVTKADIRRVANTMFQPTNRIVGMIETVSPAGG
                     PQPVTSGEGQS"
     gene            3310..4797
                     /locus_tag="VLA60_16975"
     CDS             3310..4797
                     /locus_tag="VLA60_16975"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HSF11108.1"
                     /translation="MNPSIAHQHTWCGVGRWSSLSAGLVVLLMFCVPVWVQAQVTKVE
                     ELKFPPLPELVIPEPERVVLDNGLVILLMEDHELPLVGVSAMIKTGSRLDAQETIGLA
                     SLTGTVLRTGGTKSLSGDALDEFLEGKAAAIETHIGDSSGTASMGSLTEDFPEVLKVF
                     GDVLRFPVFDPEKLKIAKNQVMAGISRQNDDPDEVTSREYKKLIYGKESPYTWEPTYA
                     SVGSITREDVIRWHATYFHPNRIILGVSGDFEREKTLALIQSIFGDWPKGPEVQDAPV
                     FIQSTVPAGVFYVEKNDMTQAKIAMGHLGIRRDDPDYYSLVIVNQILSGSFGARLFNN
                     IRSKQGLAYDVHGGVGLQWDYPGMAILSMSTKTETTGKGIDALIAEARNMVALPPTDE
                     EVASAKASILNSFVFSVDSPTKVLGKYMTYEYYGYPSTWLREFRQGIEKVTTAEVRKA
                     AQKHLRPEEFAILVVGPRAGTKPALARYEQIQELDIRIPEPTAGM"
     gene            4871..5788
                     /locus_tag="VLA60_16980"
     CDS             4871..5788
                     /locus_tag="VLA60_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSF11109.1"
                     /translation="MPIRRWVTTDRRVFFYVLLLNILIAGCQTNPYTQRSQLLLMPSG
                     QMNQMGAAQYSEVLQDPKVVISKDPREIEPVKRVAARIIEAAKRSKYGDVAKAFDWEV
                     TVIKDDNTKNAWALPGGKIAVYTGIFPMAKNEAGLAAIMGHEVVHALADHGGERMSQG
                     LVAQFGMTAAAIVLSTQGQNPALNALAMQAMGLGVQTGVLLPFSRKHESEADYIGILL
                     AGDAGYDPREAIHIWERMAAASDGAPPEFLSTHPAHETRIADLTKWMPEAMELYEKAP
                     KAPVAILPLITPSPAQRPPQHPNAARFRH"
     gene            5875..>6277
                     /locus_tag="VLA60_16985"
     CDS             5875..>6277
                     /locus_tag="VLA60_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11110.1"
                     /translation="MSLRLFWPAVVACMMLVGCGKSEAVVEIALHPTNPKILYLATND
                     YIYKSRDGGMTWQNMSAGMTHSRVIALAIDPLFPANIVAGTKGDAVYKSYDGGQRWVS
                     RRTGLDDVTISSVVHQLVFAPGSSAHIFAATS"
BASE COUNT         1422 a         1424 c         1818 g         1613 t
ORIGIN      
        1 cccgaggcga agtttgtgtt attcgaagcg tttgatgagt acacgaccaa tgtcactctt
       61 aaggtttgcg atgatgatga tgtgcttctg gccacccatt ggaatggaag gccattgacc
      121 agagaccacg gtggaccggt acgcgtaatt attcccaaac gctacggttg gaaaggggca
      181 aagtgggtca aaaaaattac gttttccgat cgcgatcaaa aggggttttg ggaagtgcgc
      241 ggatattcca ataccgcgct tccatgggac aatgaccggt atgggtgatc cggtgctgtg
      301 gccatctgta tttaagtctt tccctttctc ttctgtgcac tttcgattta gctatgtccg
      361 ttcgtgatcc tttctgtatt accaaagttg aagcgtggtc aatcgccttg ccgcttctgg
      421 caccctttgt cgtggcatca ggagccatga cggtggctca taatgtattt gtccgtgtga
      481 cgcttcgaaa ccgggcctac ggttttgggg aaatggcacc gtttcccgaa atttcaggcg
      541 aagatcaggc ggggagtctt cggagctttc ctcttgccgc aaaggcatgt cttgggcaac
      601 ctgtcactca ttacagaaaa ttagcacacc ggcttcgtga agtcgcctgg gagaatccat
      661 ctgtgcgatg tggcattgaa acggccttcc ttgatgcgtt gtgtcggggc atgggtattc
      721 ctctctgggg actgtgggga ggggccgatg tgcgaccacg ggaaatcgat gtcaccttgc
      781 caattggacc actcgatcag gtcgttgcca ccgcccggtc atggtatgcg agggaattcc
      841 ggatatttaa aatgaaaatt ggccttgagg tcgatgagga tatcaagcgg attgtggccg
      901 tgtgtgccgc atgtccccat tcgaagttta tccttgatgc taatgaggga ttttctttgg
      961 agcaggcaag tgaatgcttg aagaccatgg agcggttgcg ccttccggta atcttattcg
     1021 aacaaccggt tgcacgggag gatgttgagg gattcgtgac tttgcgacgc agaggaacga
     1081 ttccgcttgc cgctgatgaa tcagttcgat ccgtacagga cgcgcgacga ttaattgaac
     1141 gcaacgcggt tgatgtgatg aatctgaaaa ttacgaaatg tggggtagtg gagtccatgg
     1201 atattgccgg gttggcccgg gcctcgggcc tcaggctgat gattggcggc atggtcgaaa
     1261 gtcggatagc catggggtgt tctttcagtt tggccttagg gctcggaggg tttgactttt
     1321 tggatcttga catgcccctt ttattatcaa tagatccagt acagggcggg tattcgtata
     1381 agggaccggt cttgcagcca tgggaagaat ccgggcttgg gatggagatg agccaagagc
     1441 cctcatcgat gatcgtcgtt gaatagggga cgttgagtga cgatcagcat tttcaccttt
     1501 tggttgtgca cagatccctt catggcacag gcggccgtcc gatggacaac ggcttttctt
     1561 ctaagtagct tagggaaccc tttccctctg gcaagtctca ggacagggtt tgaatccccc
     1621 aataacatgg acaggtgtgg taagaatgcg gtttctgcat tcaatcgtag cttgcttgcc
     1681 agaattctca tttactgggt aggatcattt aatggccgga ttgaagatgt tggcttaacc
     1741 aggatggtga tgaatgggaa cgggtaatcg attgatggga tggagtaaga gcctcggact
     1801 tgtggggctg gtgtggtgcg tctgcgcggg gacggtgttt ggtcaggatc tggcctcctt
     1861 tgagcaacgt gtgacccggc atacgttaaa gaacgggtgg acctttatta ttgtggaacg
     1921 tcctgtggcc ccggtcttcg cctttatgac gcgagtcaat gtgggatcgg ctcaggaaat
     1981 catggggcaa acgggactgg ctcatatgtt tgaacatatg gcctttaaag gcactcctca
     2041 catcggaacg actgattacg acgcagagaa ggttgcgtta gaggcattgg aagaggctta
     2101 ccaggcttat caaacggaga aatttgctgc cgatcctgat ccggagaagg tggatcgcct
     2161 ctatgcggtt tttaaagagc gacaacaaca ggcctcttca tatgtggtga aaaatgagtt
     2221 cggcgatatc attgaacggg agggtggggt tgcgttgaat gcgtttaccg gtgctgatgt
     2281 cactggatat ttttatgctc ttccggccaa taaagtcgaa ctgtttgcgt acttagagtc
     2341 ggagcggttt ttgcacccgg tgtttcggga gttttacgaa gaacgggacg tggtcatgga
     2401 agagcgtcgc atgcgcacgg aaagtcaacc cttcggccgg ttgctcgaac agtttgttgc
     2461 gacggcttat atggctcacc cgtatcatca tccggtgatt ggttttgcca gtgacattca
     2521 atcctacaca atgaccgacg caaaaaagtt ttatgaagag cagtacgtgc ctacgaattt
     2581 ggtgacggcg atcgtcggag atgtgaaagc agaggcggtc attccgattc tcgagaaata
     2641 tttcaatcgc attcctgctt ctcccgcgcc tcctccgctt cgaatcgtgg agcctccgtc
     2701 catcgcggaa aaaattgtga agctgcagga tccttcccaa ccgctatacg tggagggtta
     2761 tcataagcca cctgtgaccc atgcggatca accggtatac gatgcgatcg acgatatttt
     2821 aacgaatggg cggacctctc gattatatcg ttcgatgatt cgggacaaac gcatcgcggt
     2881 gtcggttggg gcttacgggg aatatccggg ggacaagtat ccgcacgtgt ggatggccta
     2941 tgctgttccc ggccggggtg tgacgaatga gacggtacag caggcattac gggaagaatt
     3001 tgaccggctg aaaacgcagg atgtctccga tgaggaactg gcacgatttc gcacccgtgc
     3061 caaagcgggg ttggttcggg ctctgaataa taaccttggt ctggccatgc acctgactga
     3121 ttaccagatg ttgtttggag attggcgcga gttattccgg tccattgatc gattggatca
     3181 ggtgaccaag gccgatattc gtcgggtcgc caacacgatg tttcaaccaa cgaatcggat
     3241 cgtgggcatg attgagacgg tttctcctgc cggtggtcct caaccagtca cttccgggga
     3301 gggtcaatca tgaatcccag catagctcat cagcatacgt ggtgtggtgt aggtcgatgg
     3361 tcaagcctga gcgcgggatt ggtcgtcctc ctcatgttct gtgtccccgt ttgggtgcaa
     3421 gcgcaagtga caaaagtcga ggagttgaag ttcccgccat tgccggaact ggtcatacct
     3481 gaacctgaac gcgtggttct ggataatgga ctggtgattt tgctgatgga agatcatgaa
     3541 ttacccctgg tgggtgtgtc cgccatgatc aagacgggct ctcgattgga cgctcaggaa
     3601 accattggat tggcttcctt aacgggaacc gtgcttcgca cgggagggac aaaaagtctg
     3661 agcggagatg ccctggatga gtttttggag gggaaggccg ccgccattga aacgcacatt
     3721 ggggactcgt ccggcaccgc gtcgatgggt tctcttactg aagatttccc tgaggtgttg
     3781 aaggtatttg gagacgtttt gcggtttccc gtgtttgatc ctgagaagtt aaagattgcc
     3841 aaaaatcagg tgatggccgg gatttcccgt cagaatgatg acccggatga ggttacgtca
     3901 cgagaatata agaagctgat ttatgggaag gaatccccat atacctggga gccgacttac
     3961 gcctccgtgg ggtctatcac ccgggaggat gtgatccggt ggcatgcgac gtactttcat
     4021 cccaatcgca ttattttagg ggtgagtggc gattttgaac gtgaaaaaac acttgcgctc
     4081 atccaatcca tttttgggga ctggcccaag ggtccggagg tacaggatgc tcccgtcttc
     4141 attcagtcaa cggttccggc tggggtgttt tatgttgaaa aaaacgatat gacccaagca
     4201 aaaattgcca tggggcattt gggaattcgt cgggacgatc ctgattatta ttccttggtg
     4261 atcgtcaatc aaattctttc ggggtcattt ggcgccagat tatttaataa tatccgctcc
     4321 aagcaagggc tggcgtatga tgtgcacggt ggtgttggat tgcaatggga ctatccaggc
     4381 atggccatcc tctcgatgtc gaccaaaacg gaaacaacgg ggaaggggat tgatgcctta
     4441 atcgcagagg ctcggaacat ggtcgctctg cctcctacgg atgaagaagt ggcgagtgcg
     4501 aaagccagta ttttaaattc ctttgtcttt tcggtggatt ctcccacgaa agtgcttggt
     4561 aaatatatga catatgaata ttatggatat ccttcgacct ggctaagaga atttcgccag
     4621 ggtattgaaa aagtcaccac ggctgaagtc cgaaaagctg cacagaaaca tttgcgtccg
     4681 gaggagtttg cgattcttgt ggtgggacct cgtgccggca ccaaaccagc attggctcgc
     4741 tacgaacaaa tccaggaatt ggacattcgt attccagagc ccactgccgg aatgtaatgg
     4801 tcgcctgaat gtcggtagga tggtaatggc gtcctcttgc tgcgctatac tgaattcttg
     4861 agggtttgag atgccaatac gccggtgggt caccacagat cgtcgggtct tcttctatgt
     4921 cctgctcctg aacatcctaa tcgcgggatg ccaaaccaat ccctatacgc aacgttccca
     4981 actgttattg atgccgtccg ggcaaatgaa tcagatgggg gctgcccagt atagtgaagt
     5041 tttacaggac cccaaggttg tcatttccaa agatcccaga gagattgagc cggttaaacg
     5101 tgtcgcggct cgtattattg aggcggccaa gcgctctaaa tatggggacg tggcgaaagc
     5161 gtttgattgg gaggtgacgg tcattaagga cgacaatact aagaatgcat gggctttgcc
     5221 gggtggaaaa attgcggtct ataccgggat ttttccgatg gcaaaaaatg aggccggatt
     5281 ggcagctatc atggggcatg aagtcgttca tgcgctggcg gaccatggag gagagcggat
     5341 gagccagggg ctggtggctc aatttggaat gaccgcagcg gcgatcgtac tttcgaccca
     5401 aggccaaaat ccggcattaa acgcgttagc catgcaggcg atggggcttg gtgtgcagac
     5461 gggtgttctg ttaccgttca gccgaaaaca cgaatcggaa gctgactata tcggcatcct
     5521 gcttgccggc gatgcagggt acgatcctcg ggaagcgatt catatttggg aacggatggc
     5581 cgcggcttct gatggcgctc ccccggaatt tttatctacc catcccgcgc atgagacccg
     5641 gattgccgat cttaccaaat ggatgcctga ggccatggag ctctatgaaa aggctccaaa
     5701 ggcgcctgtg gccattctgc cccttattac accatcccct gctcaacgtc ctccacaaca
     5761 ccccaacgcg gcccgtttcc gtcactagcc atggtgaaag aagcgtatgg tcttcaaaac
     5821 tttctggcgc tttctgatca cagaggtgat cgtcattcaa ggcaggctat gatcgtgagt
     5881 ctccgtcttt tttggccggc agtggttgcc tgcatgatgt tggttggctg tgggaaaagt
     5941 gaggcggtgg tggaaattgc ccttcatccc acaaacccca aaattcttta tcttgccacc
     6001 aacgattata tttataagtc ccgggacgga ggcatgacct ggcaaaacat gtctgcgggt
     6061 atgacccatt ctcgcgtcat tgctctggcc attgatcccc tgtttccggc caatattgtc
     6121 gccgggacta aaggagatgc ggtgtataaa agttatgatg gcgggcaacg atgggtgtca
     6181 cgacggacgg gattggatga tgtgacgatt tcctcggtgg tccaccaatt agtgtttgct
     6241 ccgggatcca gtgcccatat ttttgccgcc acatcat
//
LOCUS       DATIAL010000556        11591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1281337, whole genome shotgun sequence.
ACCESSION   DATIAL010000556 DATIAL010000000
VERSION     DATIAL010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281337"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..625)
                     /locus_tag="VLA60_16990"
     CDS             complement(278..625)
                     /locus_tag="VLA60_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11111.1"
                     /translation="MKNLLKMVGLSAFLVLTSVSVYAEQSEMNRQGDQSNDPAMQNKQ
                     HSGSSIESKSPSSQNTEPMESEEQKAARPGGVGEEGSSFGSPSSLPPGETSPGYTGED
                     KKAEEMEKRQQGM"
     gene            complement(716..2128)
                     /locus_tag="VLA60_16995"
     CDS             complement(716..2128)
                     /locus_tag="VLA60_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11112.1"
                     /translation="MARSVILAGIPLLTLPLLAEAGMSRDRSDREESERSSSMEQIVQ
                     GEISKKNGDQITLRASNGERITFRINDDTNRLCPQGKTSKMSQGSSGHDSSSSEKTSS
                     SSLFGNASGQVSSQTSSGTQASSSKAGFAFGECNFEKGEFVKAKVDEDGNATFVRSMG
                     QNDDNYFDAGRMGDEYFVLPAGQLGGLDISDKDAHYSVKSSDGQEIGSVYRVMTNSNG
                     DLTYAIVRKADGQLISVPWQALEGSGNKSFKLNVTQNQLGNLPILEEGENAGAHVQRH
                     WDLTEQERQRLAKWNERLDRFYAKRDRYYEDTPTFEGNREGYDRYDSDRSRMSRSGND
                     RPQHYQDQNAEDFRTRRDSFSPTEFPPHDRAFQYEGADQRSSYNRSNRDRGNYRSQSR
                     SDMRDYDEGEYRTRGNQRSMYSQSRERNYRDPMERLSDRYDTTTSRRQYDPQRQGADQ
                     YQSRRSGGKDYDYRPDGYRP"
     gene            2359..2718
                     /locus_tag="VLA60_17000"
     CDS             2359..2718
                     /locus_tag="VLA60_17000"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSF11113.1"
                     /translation="MTTKIRQVLSSSSIVGTTVQNMGREDLGEIKDLMIDLSGGRIAY
                     AVLSFGGFLGMGDKLFAIPWEAFQVVQEEEVLLLNVAKEKLEQAPGFDKDNWPDMADM
                     TWGKSIHTYYGYDPYWD"
     gene            complement(2845..4389)
                     /gene="hpnR"
                     /locus_tag="VLA60_17005"
     CDS             complement(2845..4389)
                     /gene="hpnR"
                     /locus_tag="VLA60_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HSF11114.1"
                     /translation="MKILAVHPGPLMYTKIFLRLEPLGLELVAGAARQAGHDVRLIDL
                     QVETHRDYFRMLKEWCPDVVALSCNYLPNVPEIIDLAKMTKASFANIFIVAGGHSLSF
                     VAREVLDHAEGAIDAIVKGEGEPVIGPLLNAIQHDRYNVHRLPGVVTPEAEGPPPEFV
                     KSLDEVRPARHLLRHRSKYFLGVLDPCASIELTRGCPWDCSFCSAWTFYGRSYRPISP
                     EVVVEDLAGIREPGVFLVDDVAFIQAEHGMAIGEAIARRGIKKSYYLETRGDVLLRNK
                     EVFRLWKELGMKYMFIGLEAIDQDGLIQHRKRISLGKNFEALEFARSLGIIVAINIIA
                     DPAWDKDRFETVRQWCLEVPEVVNISVNTPYPGTETWHQESRRVQTRDYRLYDIQHAV
                     LPTRLPLPEFYEELVKTQRILNHKHLGWSALKGTMRIAAGHLLRGQTNFVKMLWKFNS
                     VYNPQLQISDHQRPVAYELSIPSSSHPSQDHPLLFIHKGKGRAGRALDHTTEEFVDST
                     RNGVSP"
     gene            4553..4903
                     /locus_tag="VLA60_17010"
     CDS             4553..4903
                     /locus_tag="VLA60_17010"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HSF11115.1"
                     /translation="MVDTSIKKVQSDHSPKGSMGQKYLATGVGIAMRLWEQLPVGQTQ
                     ESSSRDYETIGYVIRGRAELQLEGQTILLNAGDCWVVPKGASHSYTILEPFTAVEATH
                     PPAHVHGRDEVPPS"
     gene            complement(4945..5751)
                     /locus_tag="VLA60_17015"
     CDS             complement(4945..5751)
                     /locus_tag="VLA60_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HSF11116.1"
                     /translation="MSMAMGCETVPYTDRSQLVVVPQSQATEMGEQAFQQILSDPQVQ
                     QSQSPEEQEAVKRVADRIIRAAKESKFAEDAKAFKWEVTVIKDDQTKNAFALPGGKIA
                     VYTGIFPVAKTDAGLAAILGHEVVHALARHGSERMSQEVLAQAGLSAAAVGLGASGAG
                     PVVGQATMAALGLGTKVGILLPYSRTHESEADSIGLILAARAGYDPQEAVRVWQRMQQ
                     GSEGAPSEFLSTHPSHETRITRLQEEMPEALKIFRKVEHAPEHELPAVNP"
     gene            7017..7349
                     /locus_tag="VLA60_17020"
     CDS             7017..7349
                     /locus_tag="VLA60_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11117.1"
                     /translation="MKRFFTVCMATAFMLGTTSMGMAGQGMKAGSAEENVPDNIQRST
                     PSAEPSLDSGSGPGSRSDQLTEMEDMDPKNPNLSKKEGKAAQAAKDLKNKDQKSPISS
                     QSSSQKSH"
     gene            7563..8243
                     /locus_tag="VLA60_17025"
     CDS             7563..8243
                     /locus_tag="VLA60_17025"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HSF11118.1"
                     /translation="MLRDLNKIKNCVLQGTDGIIGTVADVYANDQTWKIHYLAADTGN
                     WLQGRHVLISTQSFVHPHMDVGSFRALVSKAQVEGAPEFEIEKPFSWEKEREICNYYQ
                     WSTYFPIDERELSVFPGTLTSANGLKDFSLQATDGEIGKIVNFLIDDANWTVRYLVLD
                     TSKWLAGRKVLISPMWNKSINWAERMMVVDLNQDQIEKSPEYDPAAPIERVYEINLYK
                     HYGKPHYW"
     gene            8442..9893
                     /locus_tag="VLA60_17030"
     CDS             8442..9893
                     /locus_tag="VLA60_17030"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HSF11119.1"
                     /translation="MYEHLVIFGATGDVSKRYVFPALAQVFANRGLPSEYGITGVGRR
                     DWNTPQLQEHVSELLANHHTPPPPRSREGFLESLEYARVEDLADTGQIQSLFQKRAES
                     TLLYLGLPPQMFTPVLESLRQVSLPPGTRLIIEKPFGLSYAQSQELNRLVHRKFSEEC
                     IFRIDHFLGMPIVCTIFGLRFANPVFTPIWNRHHINKIEVIWDETLTVEGRADYYDRA
                     GALKDMIQNHLLQLLAVLAHEPLETMWSPDFRDKRVELFKSVRTLSRAEIRAQTVRAR
                     YRSGEIGGVRVPGYAKSKGVVPERNTETFAQVTLWVDNERWQGVPFVLRSGKALNRDR
                     KEIRVHFKPGQKDPESFLSEEGGNILCLNLASEEVNLGIFTIQDAGGVTPAPMRVDDP
                     ISPEHLSPYERLFLEAMSGKPQLFVRDDEVEEMWNIIQPIADAWADNVVPLQSYPAGS
                     NGPPREDTGALTEYGHSRTTAETPGSDPFPSYR"
     gene            complement(9890..10360)
                     /locus_tag="VLA60_17035"
     CDS             complement(9890..10360)
                     /locus_tag="VLA60_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HSF11120.1"
                     /translation="MDVVSELDWHQIWSHFYHLIIAFLLAVPLGWDREQESRGAGLRT
                     FPLVALGSCAYMLTGIHVLTSTDAEGRVIYGILTGIGFIGGGAILKDRGNVAGTATAA
                     SIWNTGAIGMAVAWDRYEIAMVLSALNFLTFRFMPLVKKHVTPTPSKPDRTMES"
     gene            complement(10473..>11591)
                     /locus_tag="VLA60_17040"
     CDS             complement(10473..>11591)
                     /locus_tag="VLA60_17040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HSF11121.1"
                     /translation="RNIHLWEIVAVRELCLLALGVAVLWLCFQLQAILVPVFIGLGLA
                     YLTEPALSYVEQTWKLPRWLAVTFMALVICALVTGLVLWVGPNLVEQLRQFIEQVPTY
                     LSLLADRHGIDVKEISRNFLEVSANIRDNPLSTLKTFLTGTGQVFYFTNLIIGTLGSF
                     LFSFSLVIIYFFFFAWSFPSIQHTVWSVVESQEDDRWPKLLSKMDRAIGEFFRGRLLI
                     ALMMSVMFGIGWFWVGMPYWILLGFGAGLLSLVPYAATLMWPVAILLKYLDMSMGSGS
                     SDNYWMELLVWPSAVYLGVQFLEGWVLTPWVQSQSTDLSAATILLVVLIGGALGGVLG
                     LILAIPFAACLKILAQELIIPRIQRGNITEVHHEIYTP"
BASE COUNT         2972 a         2804 c         2852 g         2963 t
ORIGIN      
        1 cctctgatag gtgcagaggt catgaagact ttcaacatcc gggaagggtc aaggccatta
       61 gggctggatg tttaggtttc caaacgagag ttgaggcatt aacaagaaaa tcaatcagat
      121 tgtcgatgtg tttttaacgg agatgaaaga ctcttttacc aaatgggtat cggccgatcg
      181 tccgatctcc ccttcgtccg accggcccct acccctcact agatggcatt ccctgtcccc
      241 tagtgattct tgtatgtcat ggagcgtttc aatctcatta cataccttgt tgacgttttt
      301 ccatttcttc tgccttttta tcctcacccg tatatccggg cgaagtttcg cctggtggta
      361 ggctgcttgg actaccaaag ctggatcctt cttcgccgac tccgccgggg cgggcagctt
      421 tttgttcctc agattccata ggttccgtat tttggctaga gggacttttg gattctatcg
      481 aggacccact gtgttgttta ttttgcatag ccggatcatt agattggtca ccctgtcggt
      541 tcatctctga ttgttctgca tacacagaaa cactcgtaag aacaaggaag gctgagagtc
      601 caaccatttt caataaattt ttcatttcaa ttccccttca tttttaatgt gtcttttgga
      661 gattcaagta cccgtgaccc cctgagcctt taagacccag ggggtgacgc tttcgttatg
      721 gtcgatatcc atcaggtcgg taatcataat ctttcccgcc ggaacgccgg gattgatact
      781 gatcggctcc ctgcctctga ggatcatact gccttcttga ggtggtagtg tcgtaccggt
      841 ccgacaaccg ctccatggga tcccggtaat ttctttcacg actttgagaa tacatggacc
      901 gttgattccc gcgagtccgg tattcaccct catcgtaatc cctcatgtct gatcgggact
      961 gactccgata attgccacga tcccggttac ttcggttata ggaactgcgc tgatcagccc
     1021 cttcatattg aaaggcacga tcatgcggag ggaattcggt ggggctgaaa ctgtctcgtc
     1081 ttgtccgaaa atcctccgcg ttttgatcct ggtaatgctg aggtcgatca ttcccggaac
     1141 ggctcatgcg agaccgatca gaatcgtatc ggtcatagcc ttcccggttc ccttcaaatg
     1201 tcggtgtatc ctcataataa cgatcgcgtt tggcatagaa ccgatccaat ctttcattcc
     1261 atttcgctaa tcgctgtcgt tcctgttccg ttaaatccca atgtctttgg acgtgggccc
     1321 cggcattttc cccctcttcc agaatgggaa gattgcctaa ttgattttgg gtcacattca
     1381 gcttaaatga cttattgccg gatccttcca gcgcctgcca tggaacggag atcaattgtc
     1441 catctgcctt tctcacgatg gcataggtca gatctccgtt actgttcgtc atcactcgat
     1501 acacacttcc gatttcctgg ccgtcggacg atttaacgga ataatgggca tccttgtccg
     1561 aaatatccag gccacctaat tgacctgctg gcaggacaaa atactcatcg cccattcttc
     1621 cggcatcaaa ataattatcg tcattctggc ccatcgaacg aacaaaagtg gcgttgccat
     1681 cttcatcgac tttggccttc acaaattctc ctttttcaaa gttgcattcg ccaaatgcaa
     1741 atccagcctt ggaagaactt gcctgtgttc cggatgaggt ctgacttgaa acctgcccgc
     1801 tcgcatttcc gaacagacta ctactcgaag tcttttccga acttgatgaa tcgtggcctg
     1861 aggatccttg gctcatcttg gatgtctttc cttgtggaca cagacggttg gtatcgtcat
     1921 taatgcgaaa tgtaatgcgt tctccattac tcgcgcgaag ggtgatttgg tcgccgttct
     1981 tctttgagat ttctccctgg acaatttgtt ccatggaact tgaccgttcc gattcctcgc
     2041 gatctgaccg gtcgcgtgac atgccggctt cggccagtaa cggtaatgtc agcaatggaa
     2101 tgccggcaag gatgactgat ctggccatgt tgttcctcgt gaacataatg tggcacctcc
     2161 ttttacaaag ttaaaaaatt gtacagtgat tactttcctc aggataatgg atttatgcac
     2221 ccgtacaact ggagaaaacc ctggtttgat atttcttttt ttccagggtt tatcccagtt
     2281 tggtcatgta ggaagaacat gtaatatccc gtcagattgt ttcttttatt atccatgccg
     2341 gtctacaagg agggtgttat gaccactaaa attcgtcagg ttctttcatc aagttctatt
     2401 gtcggtacca cggtccagaa tatgggaaga gaagacctgg gagaaattaa agatcttatg
     2461 atcgatctct caggcggacg cattgcctat gcggtgttat cgtttggcgg atttttagga
     2521 atgggagata aactgtttgc gattccctgg gaggcattcc aagtcgtaca agaagaggaa
     2581 gtgttgttat tgaatgtggc caaagaaaaa ttagaacagg ctcccggttt tgacaaagat
     2641 aattggccgg atatggctga tatgacctgg gggaaaagca ttcacaccta ctatggttat
     2701 gacccctatt gggactaatc ccgtgaaatt cctgaaaaat ttccaagtga tggaagactg
     2761 ctccgcaaga acccgcctga atggcgggtt cttgctgcgg aatccaatcc tcttttaaga
     2821 cccctctctt cctccagtga aaatctacgg actgacccca ttcctggtcg aatccacaaa
     2881 ttcttccgtt gtgtgatcta acgcccggcc tgcccggcct tttcctttat ggataaataa
     2941 gagggggtga tcctgtgacg gatggctgga agaaggaata gacaactcat aggcaacagg
     3001 ccgttgatga tccgagattt gcaattgggg attgtagaca ctgttaaatt tccacaacat
     3061 tttcacaaaa ttggtctgcc cgcgaaggag gtggccggct gcaatccgca tggtgccttt
     3121 tagggcactc cagccgagat gtttgtggtt gagaattcgt tgggttttaa ccagctcctc
     3181 ataaaattcc ggcaacggta agcgggtagg gagcaccgca tgttggatgt cgtaaagccg
     3241 gtaatcccgc gtctgaactc tccgagattc ctggtgccac gtttccgtcc cgggataggg
     3301 tgtgttgaca cttatattga caacctccgg gacctccaaa caccattgac ggacggtttc
     3361 aaatcgatcc ttatcccagg cgggatcggc aatgatgtta atggccacga taatacccag
     3421 agagcgggca aattccaggg cttcaaaatt tttgccgagt gagatgcgtt ttcgatgttg
     3481 gatcaatcca tcctgatcta tggcctccag ccctataaac atatacttca ttcccagttc
     3541 tttccacagg cggaagactt ctttatttct caacaggaca tccccacggg tttccagata
     3601 ataggacttt ttgatgcccc ggcgtgctat ggcttcacca atagccatgc cgtgctctgc
     3661 ctggatgaag gccacatcat ccaccaaaaa cactccaggt tcacgaatgc ccgccagatc
     3721 ttccacgacc acctcaggac tgatcggacg ataacttcgg ccataaaatg tccaggcact
     3781 acaaaatgaa caatcccagg ggcatccgcg agttaattca atggatgcgc atggatccaa
     3841 tacaccaaga aaatatttgc tccggtgacg aagtaaatgc ctggccggcc tcacctcatc
     3901 cagagatttg acgaattcag gaggtggtcc ctcggcttct ggtgtgacca caccgggaag
     3961 ccggtgaaca ttgtagcggt catgttggat tgcattgagc agtggcccga ttaccggttc
     4021 gccttcccct ttgacaatgg catcgatagc tccctctgca tgatccagga cctccctggc
     4081 gacaaaagaa aggctgtgtc ccccggccac gataaaaata tttgcaaatg aggccttggt
     4141 catctttgcg aggtcgatga tctcgggaac attcggcaaa taattacaag agagcgcaac
     4201 gacatcgggg caccattcct tcaacattct gaaataatcc cggtgtgtct ccacctgcaa
     4261 atcaatgagc cgcacgtcat gaccggcctg tcgggctgct cccgcgacca gttccaatcc
     4321 caagggctcc aaacgtagaa aaattttggt atacatcaac ggaccgggat gaacggcgag
     4381 aattttcaag atgaacctcc tttacatcta ctacatctac taaaggacag cgctttcgaa
     4441 attccacatg gattgcgaaa aatcagccag ggttttttag agtatatgcc attggtgcaa
     4501 tcagttatgg tgtagtcaga caaccaccta cagataggag gcgtgggcac tcatggtcga
     4561 tacgagtatt aaaaaagtgc aatcagatca ttctcctaag ggttccatgg ggcagaaata
     4621 tttagccacc ggcgttggaa tagccatgcg attatgggaa caattgcctg ttgggcaaac
     4681 acaggaatct tcttccaggg attatgaaac catcggatat gtcatccgtg gtcgagctga
     4741 gctgcagttg gaaggtcaaa caattttgct gaatgcggga gattgttggg tggttcccaa
     4801 aggcgccagt cacagctata ccattcttga accatttacc gccgttgaag cgacccaccc
     4861 cccagcccat gtgcatggtc gcgatgaagt ccctccctct tgatgtgcct gctgtttctg
     4921 ccacagggaa atgaggtgga gccattatgg attgacagcc ggaagctcat gttctggcgc
     4981 atgttccacc tttcggaaga tttttaaggc ctcaggcatt tcttcttgga gacgtgtaat
     5041 tctcgtctca tggcttggat gggtggaaag aaattcagag ggggctccct cacttccttg
     5101 ttgcatgcgt tgccacaccc gaaccgcttc ttgaggatca tagccggccc gggctgccag
     5161 aatcagtcca atggaatcgg cttcggattc atgcgttcga ctgtagggga gtaggatgcc
     5221 gacttttgtc cccaaaccta aggcggccat cgtggcctgc cccacaacag ggcccgcacc
     5281 acttgctcca agccccacgg ctgcagcgga tagcccggct tgggcaagca cctcctgact
     5341 catacgctct gaaccatggc gcgcgagggc atggacgacc tcgtgtccaa gaatggccgc
     5401 cagtccggca tcggtttttg caacggggaa gattcccgta tacacggcaa tcttcccgcc
     5461 ggggagggca aaagcatttt tggtctggtc atctttgatc acggtgactt cccatttgaa
     5521 ggccttggcg tcttccgcaa atttggattc ttttgccgct cggatgatcc tatccgccac
     5581 acgttttacg gcttcttgtt cctcagggga ttgagattgt tggacctgag gatcggaaag
     5641 aatttgttgg aaggcctgtt cacccatttc tgtggcctga gattgcggaa ccacaacaag
     5701 ctgggagcga tcggtatacg gaacggtttc gcaacccatc gccatgctca tcaacatcat
     5761 caaagaataa aaaggtttaa gaagaggaag cgagggaaac atgggtcggt tgtgcataaa
     5821 tttactctca ctttaatggc taaggaatca aaacaaatca aatgaattct taaaaagaat
     5881 gagggaaccg gatggtggaa tgcaaaagac aagtgactct ttgccggtgg acaaaatgtc
     5941 catagggcca caatcactct cccttctacg atgccggatt cggaagaaag ccagagtcag
     6001 tattgaatga ggagagaaga gcacacagac gaggaaaaga atacaaacga ggacgagaat
     6061 acttcaatcc ataaagagcg aaggggcttc gatgaggaat agctttacgc ggatccaggg
     6121 taagccggac ggtggatgcc gcttcagcgc ccaatcaaat cgagggctgt ttttagattt
     6181 tccatggaat agggttttac cagagtggtg gtcactccga gggagaacgc atggcacagt
     6241 ccgatcagaa tgcgtcgtcg tgaaaatgat ggaaatagtt gagatatcgt cgatcgaggt
     6301 gtggatgaat tgaaagtcat tcatttccgg catccggaaa tcaccaatca caagatcaaa
     6361 tcgtgaggag cgccatatgt aaagaccttc gtgactacca gccccctcct gacacacata
     6421 tccttacgag tacaatctgg ttgttaaaaa atcccgatgg tccggattgt cctccaaaat
     6481 taaaattctc atcttaagtg gcttttgaga tatgtgggag acggacttaa agttgcagta
     6541 aggggccggg gaatgtgacg atgccctaaa tgagtcaatg cttttctttt cataatcctc
     6601 ctcgtcaata aagatgttcg tgtctttcct gacttaaacc ttccttctca gctccttccc
     6661 gttactggtc aggatagaag tgcataggct ctttcatcat tggcactatt tgatgtgaat
     6721 gaccgtgaac aacacaacgg aagagatttt ggatttcgtc ttcacgtggt tggcagttgt
     6781 acacgacata ttcttctcat tcgccctggt aacatttggc attggtaatg tgggtaaacc
     6841 ttatacattt ggttttttct gattgccgat ttgtccattg gtctgtttgc ggcgatcatg
     6901 ctactgataa agtacagacg gcaattgaga acctgatagg aaggtttcag cttttgatgt
     6961 tggagcctca acagtttttc aattgaaagt tatttcactt tttcaggagg tatgtgatga
     7021 aaagattttt tactgtgtgt atggcaacgg cgttcatgtt gggtacgaca tccatgggaa
     7081 tggccggtca gggaatgaag gccggttccg ctgaggaaaa tgtccctgac aacattcaac
     7141 gaagcacgcc gtccgctgag ccttcactcg acagtggaag tggtccgggg agccgatcgg
     7201 atcaattaac ggaaatggag gatatggatc ctaagaatcc caatttgtcc aagaaggaag
     7261 ggaaggccgc acaggccgcc aaggacctga agaacaagga tcagaaatct ccaatttcca
     7321 gtcaaagttc atcacaaaaa agccactgat gcactgagtg aaatggcagc gtgtcgcgtg
     7381 tcatgagttg gccgtcaatc cggtaaacca aattgacggc caattccttg gttcacaacc
     7441 ttcgtgctga ctcatttcgt ggatgtagat tcgatgtccg tatagctggt gttaatggtc
     7501 gtctgacagg gtcgaacggc cactgcttta tacttacagc ttaatcaaga agaggaaatg
     7561 acatgttacg agatcttaat aaaattaaaa actgcgtgct ccagggtaca gatggaatta
     7621 taggtaccgt ggcagacgtc tatgccaatg atcagacatg gaaaatccat tatctggctg
     7681 ctgacacagg gaattggttg cagggccgac atgtgttgat ttctacccag agttttgtgc
     7741 acccacatat ggacgttgga tcgttccggg ccttagtcag taaagcgcaa gtcgagggcg
     7801 cgccggagtt tgaaatcgaa aaaccattct cctgggagaa ggaacgggaa atctgcaatt
     7861 attatcaatg gagtacctat tttccaattg acgagaggga actttcggtg ttccccggga
     7921 cattaaccag cgcgaatggg ctcaaggatt tttcccttca ggcgacagat ggtgaaattg
     7981 gaaaaattgt gaatttcctt attgatgatg ccaattggac cgtgcgctat ttggtcctgg
     8041 atacttccaa atggcttgcc ggcagaaagg tgttaatcag tccaatgtgg aataaatcca
     8101 ttaactgggc agagagaatg atggtcgtgg acctcaatca ggaccagatt gaaaaatctc
     8161 cggagtatga tccagccgcc ccaatcgaac gggtgtatga aataaatctg tataaacatt
     8221 acggtaaacc acactactgg tagcgaacta aagagaaagc acatgccaag atgatggagg
     8281 tgttggggag gaacatcctg caaaaagggc tagccatgtt atttcatgcg gcgataaagg
     8341 accgttcttt ggttaaagaa atttcctccc gacgttctcc tgagtgccct tacgtgtgac
     8401 gccccgattg tctttcttga agtgaggttc ataaagaccg tatgtatgag catctggtca
     8461 tttttggagc gacaggcgac gtctcgaagc gttatgtttt tcctgcctta gctcaagtgt
     8521 tcgccaaccg gggtcttccc tcagaatatg gaatcaccgg agttggacgg cgagattgga
     8581 atacgcctca gcttcaagaa catgtgtccg aattactcgc aaaccatcac acgccacccc
     8641 cacccagatc acgagaagga tttttggaat cgttggagta tgcacgggtc gaagatcttg
     8701 ctgacactgg acagatacag tcgctctttc agaaacgggc cgagtcgacg ctgctatatt
     8761 taggactccc tccccagatg tttactccgg ttttggagtc cctacgacag gtatccttac
     8821 cgccagggac ccgtctcatc atcgaaaaac ccttcgggtt aagctatgcc caatcacagg
     8881 aattgaaccg gcttgtgcac cggaaatttt ctgaagaatg catatttcgc attgaccact
     8941 ttttaggaat gccgatcgtg tgcactattt ttggtctccg gtttgccaat ccggttttta
     9001 cgcctatctg gaaccgtcat cacatcaaca agatcgaggt gatctgggat gagaccttga
     9061 cggtggaagg gcgtgcggac tattacgatc gtgccggagc cttgaaggat atgattcaaa
     9121 atcacctcct tcagttgttg gctgtcctgg ctcatgagcc cttggagacc atgtggtctc
     9181 ctgattttcg ggacaaaagg gtggaattgt tcaaatcagt gagaacactg tcaagggctg
     9241 agattcgagc tcagaccgta cgagctcgtt accggtcagg ggagataggt ggtgtgcggg
     9301 ttcccggtta tgcaaaaagc aaaggggtgg ttcccgaacg aaacaccgaa acgtttgctc
     9361 aggtgaccct ctgggtcgac aatgaacgct ggcaaggcgt gccttttgtg ttacgttctg
     9421 gaaaagcgtt aaatcgagat cgaaaagaaa ttcgagtgca ttttaaacct gggcagaaag
     9481 atccggaatc tttcctttcc gaagaaggtg ggaatatctt gtgcttgaat ctggcgagtg
     9541 aagaggtcaa tcttgggata tttactatcc aggatgccgg aggggttaca cctgcaccga
     9601 tgagggtgga cgaccccatt tctcctgaac atctttcccc ctatgaacgg ttgtttctcg
     9661 aagccatgtc cggcaaacca caactttttg tgcgggatga tgaggtcgag gagatgtgga
     9721 atatcattca acccattgcc gatgcctggg cggacaatgt ggtaccctta cagagttacc
     9781 cggccgggtc caacggccca cccagggagg ataccggggc gttgacagag tacggccatt
     9841 cccggacgac agccgagact cctgggagcg acccattccc atcctatcgt taggattcca
     9901 tggttcgatc gggttttgat ggtgttggtg tgacatgttt tttaaccaac ggcatgaacc
     9961 gaaacgttaa aaaattcaat gcgcttaaca ccattgcgat ttcgtaacga tcccaggcca
    10021 ccgccattcc gatggctccg gtgttccaaa tacttgccgc ggttgcggtt ccggccacat
    10081 tccctctgtc tttgagaatg gcgccaccgc caataaaacc gattccggtt aagatgccat
    10141 agatgacacg tccttcggca tcggtggacg tcaacacatg gattcccgtc aacatatagg
    10201 cgcatgagcc gagtgcaacc aggggaaacg tgcgcagccc tgcaccgcgc gattcttgtt
    10261 cgcgatccca accgagagga accgccaata aaaaggcaat gatcagatga tagaaatgcg
    10321 accaaatttg atgccaatcc aattcagaga ccacatccat cgtgaatcct ttcccttctc
    10381 tatttggtcg aactcaaacc gtgaagactg ttcatcatga tgggaagagt ttccatagaa
    10441 tatatgaccg aagttaaatc gagtcggacg cattagggtg tataaatttc atggtgcacc
    10501 tccgtgatat tgcctcgctg aatccgggga attatcagtt cctgggcaag gattttcagg
    10561 caagccgcga aagggatggc aagaattaat cccaacactc cgccaagtgc cccgccgatc
    10621 agcaccacca gtagaatggt cgcagcgctg aggtcggttg attggctttg gacccaaggg
    10681 gttaataccc acccctcaag aaattgcact cccaggtaga cggcactggg ccagacaagg
    10741 agttccatcc aataattgtc ggatgagccg gaccccatgg acatatccaa atatttcaaa
    10801 agaatggcga cgggccacat caaggtggcg gcataaggaa ctaaactcaa taatcctgcg
    10861 ccaaatccca ggagaatcca ataaggcatc cccacccaaa accatccgat gccaaacatc
    10921 acactcatca ttaacgcaat cagtaaccgg cctctgaaaa actctccaat ggcccgatcc
    10981 attttggaca gtaatttcgg ccaacggtca tcctcctgag attctacgac actccatacc
    11041 gtgtgttgaa tggaaggaaa gctccaggca aaaaagaaaa aatagatgat gactaaagag
    11101 aacgagaaga ggaaggaccc cagcgtgccg ataataagat ttgtgaaata aaaaacctga
    11161 ccggttcctg ttaagaatgt cttgagtgta gacaagggat tatcccgaat gttcgctgag
    11221 acctccagga aattccggga aatttctttc acatctatgc cgtggcggtc ggccaagagg
    11281 gaaagatagg tgggaacctg ttcaatgaac tgccgcaatt gttccacaag gtttggtccc
    11341 acccagagga caagcccggt caccagggca cagatgacca gcgccatgaa agtcacggcg
    11401 agccatcgtg ggagcttcca ggtctgttca acataactca gggcaggttc agtgaggtag
    11461 gccagcccca ggccgatgaa gaccggaact aaaatggcct gcaattgaaa acacaaccac
    11521 agcacagcga ctcccaacgc caggagacac agttcacgaa ccgcaacaat ttcccagaga
    11581 tggatatttc t
//
LOCUS       DATIAL010000557         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1281438, whole genome shotgun sequence.
ACCESSION   DATIAL010000557 DATIAL010000000
VERSION     DATIAL010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281438"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VLA60_17045"
     CDS             <1..379
                     /locus_tag="VLA60_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11122.1"
                     /translation="RVVLDLPEPWQVVPHAARALRSGGLYLSYVPTVPQVMQTVQALE
                     QSRVFTMVQNFETLLRTWNIKGRSVRPDHRMVAHSGFITVARKVENNIWNSEQYNAIE
                     LASQQDDHVIKEDEYVGDGLDIP"
     gene            414..788
                     /locus_tag="VLA60_17050"
     CDS             414..788
                     /locus_tag="VLA60_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF11123.1"
                     /translation="MVGPVKTILIADDEEDLRLLVQVTLEDPSYRILTARDGCAAIDA
                     VCTHIPDLLILDWMMPGLNGCEVVRKLRLHSDTAKIPIVMLTAKDGLDARKQIASLDL
                     AGYLVKPFSPLELIQKVREVLA"
     gene            788..1663
                     /locus_tag="VLA60_17055"
     CDS             788..1663
                     /locus_tag="VLA60_17055"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSF11124.1"
                     /translation="MSEDKPQLDGVPPLSLITYSEAEASQVSATGKLQAARSQLMAYA
                     KDLKQMLEQESQKTQLLKKAHAQMLAYARDLKLSLEAEQQKNSELEQAYADTILRLAR
                     ASRYKDEETGAHIERLSHYSQSLALAIGWDQPRARCLFAVAPMHDVGKIGVPDAVLGK
                     NGPLTEEEWQSIKQHPLLGASLLDGSASPLIEMAKEVALTHHERWDGSGYPRGLKGTQ
                     IPITGRIVMLCDLYDALRSRRPYKPAFTHEKTCDIILNGNDRTKPTHFDPHLLEAFRE
                     MHQDFNGIYSTVAEI"
     gene            complement(1690..2109)
                     /locus_tag="VLA60_17060"
     CDS             complement(1690..2109)
                     /locus_tag="VLA60_17060"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HSF11125.1"
                     /translation="MKRFSFSLTIALLFLGLWIPAIVTAEPYLLTVQQLKAGLEKASQ
                     SKQKGFVLVDVRSPDEHMSGFIPGTDFNIDFREMQGRHQELKAKLDQHIVVYCQSGHR
                     SNIAAETLMGLGYQHVYNVEGSMNAWTEAGYPIEHPK"
BASE COUNT          643 a          624 c          688 g          682 t
ORIGIN      
        1 tcgagtggtg ttggatcttc cagagccatg gcaagtggtt ccgcatgcgg cacgggcatt
       61 acggtccgga ggtctgtatt tgagttatgt cccgacggtg ccgcaggtga tgcaaacggt
      121 acaggctttg gaacagagcc gggtttttac catggttcaa aattttgaaa cgttgttgcg
      181 gacgtggaat attaaaggcc ggagtgttcg acccgatcac cgaatggttg cccattccgg
      241 ttttattacc gtagccagaa aagtcgaaaa taatatttgg aattctgagc agtacaacgc
      301 tattgagctt gcgtctcaac aagatgatca tgtcatcaag gaggatgaat atgtcggtga
      361 tggattagat attccctgag cccttgcggg ccaaccagta ggaaatgttc accatggtgg
      421 gtcccgtgaa aacgatcctc atcgccgatg atgaggaaga tcttaggctg ctggtccagg
      481 ttactctcga agacccctcg tatcggattc tcactgctag agatggttgc gcggcgatag
      541 atgccgtttg cacccacatt cccgacctat tgattcttga ttggatgatg cccggtctaa
      601 atgggtgtga agtggtacga aagcttcggc tacactccga cacggctaaa attccaattg
      661 tcatgttaac ggcgaaagat gggcttgatg cacgtaagca gatagcttct cttgatcttg
      721 cggggtattt ggtcaaacca tttagcccat tggagttgat tcaaaaagtt cgggaggtac
      781 ttgcctaatg agcgaggata aaccccagct agatggggtt cctcctttat ccttaatcac
      841 gtactcagag gccgaagctt cccaagtatc tgccacaggg aaactccagg cggcccgctc
      901 gcaactcatg gcctatgcca aagatctcaa gcagatgttg gagcaggaaa gtcagaaaac
      961 tcaactgctt aaaaaagctc atgcccaaat gcttgcttac gctcgagacc ttaagctctc
     1021 attggaggca gagcaacaga agaatagtga attggaacag gcctatgccg acactattct
     1081 tcgcttagcc cgtgcgtcac gctataaaga cgaggaaaca ggggcacata ttgaacggtt
     1141 aagccattat tcgcaatcgc ttgctttggc tattggctgg gatcagccac gcgctcgttg
     1201 tttattcgcc gttgcgccga tgcatgatgt ggggaaaatc ggggttccag atgccgttct
     1261 tggaaagaat ggcccgttga cggaagaaga atggcaatcg attaagcagc atcctctttt
     1321 gggagcgagt ttattggatg ggtcggcgtc tcccctgata gagatggcca aggaagtcgc
     1381 gctgactcat catgagcgtt gggatggaag tggatatcct cgagggctga agggaacaca
     1441 gattcccatc accggacgaa ttgtgatgct gtgcgatctt tacgatgcct tacgaagccg
     1501 aaggccctac aaaccggcgt ttacccatga gaagacatgc gacatcattt taaacggcaa
     1561 cgacagaaca aagccgacac attttgatcc acaccttttg gaagcttttc gggagatgca
     1621 tcaggacttt aacggcatct attccaccgt ggccgaaatc taacattctt tgccgcaggc
     1681 tgttcttgat tacttggggt gttcgatagg ataccccgct tccgtccagg cattcatgct
     1741 tccttccaca ttatagacat gctgataccc taaccccatt aaggtttcag cagcgatatt
     1801 gctgcggtgt ccagattggc agtacaccac gatgtgctga tccagtttcg ccttgagctc
     1861 ttggtggcgt ccttgcattt cccgaaaatc aatattgaaa tccgttccgg gaatgaagcc
     1921 actcatatgt tcgtctgggc tccgaacgtc gacgagcaca aatcctttct gtttggactg
     1981 ggaagccttt tcaagccctg ctttaagttg ctgaaccgtt aagaggtagg gctcagccgt
     2041 aacgatggcg gggatccaca atcctagaaa aagaagagcg atggtcaagg agaaagaaaa
     2101 acgtttcatg gttatcctcc gtagtatcgt agtattggga atgactttct tgtttgagga
     2161 agttaaggca gatgccgttg tccactctaa aaaggaaatt gaccagacaa tcgttgagca
     2221 attgttttaa aattgtatgg ctggtcagaa gagacggtca agtgagtcat atatggactc
     2281 ccccttgttt gcaagcagga attcatgctt ttgagagaag ggtctgcggt catatatccg
     2341 gcttcgtgag ggaacggtct tcgttccgag ccttgatggt tgtcgcgcgg cagctcctct
     2401 gcatcctggc ggctttttga ctcagccgat gacaaatctc agggtttgct cccgcgaggg
     2461 gtgaccactt acgcccatca gttggcgtct cctgctacgc aatccttgga agcgcttctc
     2521 cgttttcctt acaccgtccc gcacagtttg gcgacatcat acggctgggc tttgtctatg
     2581 agggcgaacg ccatccgcgc attcttgttc gccaaggcca caatcgcctt attcatc
//
LOCUS       DATIAL010000558         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_316234, whole genome shotgun sequence.
ACCESSION   DATIAL010000558 DATIAL010000000
VERSION     DATIAL010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316234"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VLA60_17065"
     CDS             <1..240
                     /locus_tag="VLA60_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11126.1"
                     /translation="VQYVSAMLPQLKTSEDIDFPVLTEVVWAIGKQRWESSLRPIREL
                     QQKIWVIFDNSKEMADLREATSWTYKQIDLDGHLS"
     gene            272..1372
                     /locus_tag="VLA60_17070"
     CDS             272..1372
                     /locus_tag="VLA60_17070"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HSF11127.1"
                     /translation="MRIWVSCVLTVFLFTGLLYSEGAARRTYLSPEQKTELDKIQTVR
                     VHVLALTERGKVDGTTIQDIIKERLEELGYTVVTNGDVPFDVTLNVKCEEQKKWGGTT
                     RAGGDADQVGAPARLWKGPACLFSYQLDGRDLGWYKEARTTFEDSGEAAKKANAPESG
                     TYAIQQLAERIREFDFPIMIAAEWGHDTRLLELLKNPHTSNKRKLKILALLPQVQSHE
                     ALPILKDIIQDKELAEEAILALASAGSDAIPLLTEIFQNGTDTEIRAAAARALGEVGA
                     HTGDPAITPPLLAYLKENLKNMKTSEDINFPILTEVVWGLGKLRNEKSIPPITELQTK
                     VWLIYDTSKEMEELREATNWTYKQVDMDGQIQ"
     gene            1539..1985
                     /locus_tag="VLA60_17075"
     CDS             1539..1985
                     /locus_tag="VLA60_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11128.1"
                     /translation="MSLAQVMGELAEMDRLEQRADDAIANGDPEGAALSIGKAALMAG
                     LLAEKETGQNARLLYQAVETLFRGHEQGYRAIALFERAGAQPPASKGVCQFLVHAAEM
                     VKKSRNELEALPDFSNESVENRRQRHIDRSKEWEDRLQGLQQDFSC"
     gene            complement(2204..3295)
                     /locus_tag="VLA60_17080"
     CDS             complement(2204..3295)
                     /locus_tag="VLA60_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HSF11129.1"
                     /translation="MWSNMVFPLVGALLLSGCWLGNSTPPDWILSPQTAYPADRYLVG
                     IGEGTTRDQAEKRAYAAVARIFSANVQATSLDQESYSLKEANELSSTHRSLHIDQQTR
                     VTTTKLLENVKVLEAWYRPFDQQFVILAGLDRHQTAHMLMARLNDLDKTIEGFVAEGR
                     THSQKIHRIQGYKQALALLQSRHDMNTDLRVIRASGEGILPPYTPQQLQREFMDFVAR
                     SLIISVAMEGENSDELEGAIWEGLKREGLLAGAREQEVITSETHADITISGTGRLWTV
                     DLPDPLFRYVRWCGDVQIRETESDRLVGVISRSGREGHITDKEARVRASKVMQEVISQ
                     EVARLLTHSVFSAEPDESLSQRIPNACPY"
     gene            complement(3309..>3999)
                     /locus_tag="VLA60_17085"
     CDS             complement(3309..>3999)
                     /locus_tag="VLA60_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11130.1"
                     /translation="MAQVIVVSYLGRSPQKEDLFIDVPISLDALQLVALAKTVGGPGR
                     RSTRGGEALLYGIHGQIARVALPRLVPERTKIAYNTLELTEGTTTFNAHSQRLYDIGA
                     VAEKNLDDGYSTLVLRAVARSAMKMAAAEGIGIGVHSAMHKNSQEWVGLLAVILARIA
                     ALVTEEADIRSWRTLPGEIQLIRLWVPAGSYRLTVNAVDHQGRVMALPKTEQLDLESG
                     EAHFVVRQYVE"
BASE COUNT         1011 a          984 c         1009 g          995 t
ORIGIN      
        1 gtgcagtatg tgtccgccat gttacctcaa ttgaaaacat cggaagatat tgatttcccg
       61 gtcctcaccg aagtggtatg ggcgattggc aagcaacgat gggagtcgtc cttgaggcct
      121 atacgggaac tacaacagaa aatttgggtg atttttgaca attcgaagga aatggccgat
      181 ctccgggagg ccaccagttg gacatataaa caaatagatt tggatggtca cctcagctaa
      241 tacccattat atttacgcag ggaggtcagg catgaggata tgggtgtcat gtgtgttaac
      301 ggtgtttttg ttcactggac tattgtattc tgaaggagca gcccgccgga cctatcttag
      361 ccctgaacaa aaaaccgaac tcgataaaat ccaaactgtt cgcgttcatg tactggctct
      421 gacagaacgg ggcaaagtgg atggaacaac catccaagat attataaaag aacgattgga
      481 agaattgggt tatacggttg tgaccaatgg agatgttcca ttcgatgtca ccctgaatgt
      541 gaaatgcgag gaacagaaaa aatggggagg caccactcgt gccgggggtg atgcagacca
      601 ggtcggtgcc ccggcacggc tctggaaagg tcctgcctgc ctgttctcct atcagttgga
      661 tggaagagat ctaggatggt acaaagaagc ccgaaccacc ttcgaagatt ccggcgaagc
      721 cgcgaagaag gcgaatgcgc ctgaatccgg tacgtatgcg atccaacaat tagccgaacg
      781 aattcgagaa tttgattttc ccatcatgat tgccgcggaa tgggggcatg atacccgtct
      841 tctagaattg cttaagaatc cgcacacctc caataaacgc aaactgaaaa ttttagcctt
      901 gctgccccag gtgcaatctc acgaagcgct tcctattctg aaagacatta ttcaagataa
      961 agaactggca gaagaggcca ttttggcgct ggccagtgcg ggaagcgatg ctattccact
     1021 tctgacggag atctttcaaa atggtaccga caccgaaatt cgtgcggcgg ccgccagagc
     1081 cttgggtgaa gtcggggctc atacgggaga tccggccatc actccgcccc tccttgctta
     1141 tcttaaggaa aatttaaaga atatgaaaac ttccgaagat attaattttc cgattctcac
     1201 ggaagtggtg tgggggttag gtaaattgcg caatgaaaaa tccattcctc ccattaccga
     1261 gctgcagaca aaagtctggc tgatctatga tacatcaaaa gaaatggagg agcttcgcga
     1321 agcgactaat tggacctaca aacaagtcga tatggacgga caaattcaat aactcccgat
     1381 gggagttttt gggttgatcc aatttttcat cagcctgtca ttccatgtct gagccactct
     1441 tcagccggat cgatctctcc tttttctctt tgaaactttg gcgaatcctc tcaggaagcc
     1501 tggtgtggtg tgtcctggtg cccgtatggg gaatgcccat gtccctagcc caagttatgg
     1561 gggaactggc ggaaatggac agattggaac aacgagccga tgatgccatc gccaatggag
     1621 acccagaggg ggcagccctg tcgattggga aggccgccct catggcgggt cttcttgctg
     1681 agaaggagac aggacaaaat gctcgtttgt tgtatcaagc agtagaaact ttgtttagag
     1741 gacacgaaca aggctatcgg gcgatagcac tgtttgagcg agcgggagca caaccccctg
     1801 cttccaaggg ggtgtgtcaa ttcctcgtcc atgccgctga aatggtcaaa aaatccagga
     1861 atgagcttga ggccctcccg gacttcagca atgaatccgt ggagaaccgt cgacagcgcc
     1921 acatcgacag gtccaaagaa tgggaagatc gcttgcaagg actgcaacag gatttttcct
     1981 gttagagact gctgaacaat tatgccctga gctttgccga aggagccgca agcgggaatc
     2041 tcgtttcctt ttgctataat ccgtccatgt cagaattagg ccaaatcaat ttttttattc
     2101 aacacccact gttatgtttt ttttcgtctg gcattggccc aaattcatcc acaacaatct
     2161 gctggaaatt gaaatactat gattcatact gttaagacgt gggctagtaa ggacaggcgt
     2221 tgggaatacg ttggctaagg gactcatccg gttcggcact gaagacggaa tgagtgagta
     2281 accgggcgac ctcctgcgag atgacttcct gcatgacttt gctggcgcga acccgtgctt
     2341 ccttatcggt gatatgtcct tcccggccgg accgggatat tacgccgact aatcggtcag
     2401 actcggtttc acgaatttgc acatctccac accaccggac atagcgaaat aaaggatcag
     2461 gcaaatctac ggtccacaga cgcccggttc cggaaatggt gatatcggcg tgtgtttcag
     2521 aggttatgac ttcttgttcc cgggctccgg ctaatagacc ctctcgtttt aaaccttccc
     2581 atatagcccc ctccagctcg tcagaatttt cgccctccat ggctacagag attatgaggc
     2641 tgcgtgcgac aaaatccatg aactcgcgtt gcaattgttg cggggtatag ggtgggagaa
     2701 tgccctctcc actagcgcgg atgacccgta aatccgtatt catatcatgg cgtgattgaa
     2761 gaagtgcaag tgcctgttta tatccctgaa ttcgatgaat tttttgagaa tgggttcgac
     2821 cttctgccac gaatccttca atggttttat ccagatcgtt cagcctggcc atcaacatgt
     2881 gtgctgtctg atgtcgatcc aatccggcaa gaatgacaaa ttgttgatcg aatggtcgat
     2941 accacgcttc cagaaccttc acgttttcaa gcagcttcgt ggtggttact cgcgtctgtt
     3001 gatcgatatg aagagatcgg tgcgtcgaag atagttcgtt ggcttctttg agagaatatg
     3061 attcctggtc cagagatgtc gcttgaacat tggcggaaaa tattcgtgcc acggctgcgt
     3121 aagctcgctt ttccgcttga tcgcgggtag taccttcccc tattcccact aaataccggt
     3181 cggctgggta ggctgtctgt ggtgacagaa tccagtcagg cggggtggaa tttcctaacc
     3241 agcatccgga cagtagcaag gcgcctacca atggaaagac catgttgctc cacatcccgg
     3301 gtatcctttc attcgacata ctgcctgacc acgaagtgag cttctccact ctccaaatcg
     3361 agttgttcag tcttcggtag tgccataacc cttccttgat gatcaacggc attcacggtc
     3421 aggcgataag atccggctgg aacccagaga cggatcaact gaatttctcc cggtaacgtc
     3481 cgccatgaac gaatatcggc ttcttcggtg actaaagcag caatgcgagc caggatgact
     3541 gctaacaacc ccacccactc ctgggaattt ttatgcattg ccgaatggac accaattcct
     3601 attccttccg ccgccgccat tttcatcgca cttcgagcca cagcccgcaa aaccaacgtg
     3661 gaatagccat catccaaatt tttttcggct acggcaccga tgtcgtatag acgttgggag
     3721 tgcgcgttga aggtggtcgt tccttccgtc agttcgagag tgttataagc aattttcgtg
     3781 cgttcgggaa cgagtcgggg caaagccaca cgtgcgattt gaccgtggat tccatagagc
     3841 aaagcctcac cccctctggt tgatcgccgg cctggcccac ctaccgtttt ggccaatgcc
     3901 actaattgaa gcgcatccag gctaattggc acgtcgataa aaagatcctc cttttgtggg
     3961 cttcgtccca ggtagctgac cacgatcacc tgagccata
//
LOCUS       DATIAL010000559         3648 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_744035, whole genome shotgun sequence.
ACCESSION   DATIAL010000559 DATIAL010000000
VERSION     DATIAL010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744035"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VLA60_17090"
     CDS             <1..1416
                     /locus_tag="VLA60_17090"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF11131.1"
                     /translation="QAIAILAVYGGQAIRPQLIALKRGVDIVVATPGRALDHIRRKTL
                     QLKDLQIVVLDEADEMLNMGFAEDLDAILKQTPATRQTALFSATMPPRIASIARHHLR
                     NPIEIQIIKEPVKAGVAPRVHQTAYLVARPHKVAALARILDMSSPKSALVFCRTRLEV
                     DELTSMLNGRGYRGEGLHGGMNQAQRDRVMQSFRAGKTELLVATDVAARGLDIPHVSH
                     VVNYDVPSSPEAYVHRIGRTGRAGRAGEAITLVESRERWLLQNIERLTKSKVEMGTLP
                     TGADLQAKRLEQIGVSIQEILKANDFEQFRVVVNTLTEKFDPADVAAAAIQLAHGSKS
                     GNRVEEDIPAIRERAPDTSQTSRRPRRGPSDFPDSGRALRGGGGGGRPAGRRERHSQS
                     TSMATLHVNAGRMAGVRTGDLVGAIANEAGINSNVIGPIRVSDQFSLVKVPEEHIHGI
                     IKALGRTRIKGQKVTIRLYKE"
     gene            1556..2290
                     /locus_tag="VLA60_17095"
     CDS             1556..2290
                     /locus_tag="VLA60_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HSF11132.1"
                     /translation="MPSDLTILDVRDRITKARSVTVLTGAGISADSGVPTFRGPEGLW
                     KNYRPEELASPEAFARDPKLVWEWYNWRRELVATKHPNAAHETLVQLERRIEHFWLIT
                     QNVDGLHALAGSQRLSEIHGNIWKVRCTQCNRISPNRDLPLVYPPICTDCSGLLRPHI
                     VWFGESLDQEVMNRSYQALRTCDILLIIGTSGVVYPAASFASIAKDGGALVVELNLDP
                     TPNSSVVDVAFQGRAKDLVPLLCEVT"
     gene            2375..2575
                     /gene="thiS"
                     /locus_tag="VLA60_17100"
     CDS             2375..2575
                     /gene="thiS"
                     /locus_tag="VLA60_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSF11133.1"
                     /translation="MQLTINGKPETLEVSTVLDVLKTKDIDPQLVAVELNTQMVDQEQ
                     LGTTSIKDGDKLEFLFFMGGGA"
     gene            2572..3501
                     /gene="cysK"
                     /locus_tag="VLA60_17105"
     CDS             2572..3501
                     /gene="cysK"
                     /locus_tag="VLA60_17105"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HSF11134.1"
                     /translation="MSAGFLRNITDGIGHTPLVRLNRLSPQGGATIYGKAEFYNPGGS
                     VKDRICLNMIDEAEQKGLLKPGGTIVEPTSGNTGIGLALVSAVRGYKLILVMPEGMSL
                     ERASLLSSYGAQLVLTPAREGMRGSIKEAESILDQNPEYFMPNQFSNPANPAMHRKTT
                     ALEIWEALDGKVDAFVAAVGTGGTITGCGELFKEKNPSVKVIALEPAGSPVLSGGEPG
                     PHRIQGIGAGFVPKVLNRSLIDEIMTVTDEEAYQTTKQLARKEGLLVGISAGANVFAA
                     QKVAESLGPNKNVVTILCDTGERYLSIEKYFNI"
BASE COUNT          901 a          932 c         1008 g          807 t
ORIGIN      
        1 caggccatcg cgatcctcgc agtttatgga ggacaggcca tccgaccaca gctcatcgcg
       61 ttgaaacgcg gggtggatat cgtcgtggca acgccaggac gggcactgga ccatatccga
      121 cgcaaaaccc ttcagctcaa agaccttcag attgtggtgc tggatgaagc cgatgagatg
      181 ctgaatatgg ggttcgccga agatctggat gccattctta agcaaacgcc ggcaaccagg
      241 cagaccgcac tattttcagc aaccatgcct ccacgcattg catcaatcgc acgacatcat
      301 ctgcgcaacc ccatcgagat tcagatcatc aaggaaccgg taaaggccgg ggtggcccct
      361 cgggtgcacc agacggccta cctcgtggcc aggccacata aagtggcggc cctggccaga
      421 atcctggata tgtccagtcc aaagtccgct ctggtgtttt gccgaactcg tttggaagtc
      481 gatgagctga cctccatgct caacggccgc ggataccggg gagaaggcct gcatggcggg
      541 atgaatcagg ctcaacgcga tcgggtgatg caatcgttcc gtgccgggaa aaccgagctg
      601 cttgtcgcaa cagacgtggc ggcccgggga ttggatattc ctcacgtatc acatgtagtg
      661 aattacgatg tcccgtcctc tcccgaagcc tacgtccatc gtatcgggcg aaccggacgc
      721 gccggacggg ccggtgaagc tattaccctc gtggaatcac gggaacgctg gctgctgcaa
      781 aacatcgagc gcttgaccaa atccaaagtc gagatgggca ccctcccgac gggggccgac
      841 cttcaagcca agcgccttga acagattggc gtttccattc aagagattct caaggccaac
      901 gactttgagc aatttcgagt cgtggtcaac accctcaccg aaaaatttga ccccgcggat
      961 gttgccgcag ccgccattca attggcccat ggatcaaagt caggtaatcg ggttgaagag
     1021 gacattcctg caatacggga gcgggcacct gacacatccc agacgtccag acggccaagg
     1081 cgagggccat cggatttccc cgattccgga agggctctca ggggaggagg aggcggtggt
     1141 aggcccgccg gcaggcggga acggcacagt caatctacaa gcatggcgac cttgcatgtg
     1201 aacgccggtc gaatggcagg ggtccgcaca ggagatctgg tcggtgccat cgccaacgag
     1261 gccggtatca attcgaatgt gattggcccc attagagtct ccgatcaatt ctctctggtc
     1321 aaagtgccgg aagagcatat ccatggcatt atcaaggctc taggccggac aagaattaaa
     1381 gggcaaaaag tcaccatccg cctctataag gaataagaga agtcctgaca ccggaagtaa
     1441 tggaatgttc tcaatagtgt gcttgggcca taaatcataa cgaggattaa aaatcgccat
     1501 aagaacctga catggcgtct gtctgtctgt ctcactctga atttgaattt atgttatgcc
     1561 tagtgatctg acgattcttg atgtacgtga ccgtattact aaagcccggt cggtcacggt
     1621 ccttaccggc gcgggaattt ccgcggatag cggcgtgccg acgtttcgcg gacccgaagg
     1681 cttgtggaaa aattaccggc cggaagaact ggcatcgccc gaggcctttg cccgggaccc
     1741 gaaacttgtt tgggagtggt ataactggcg gcgggaattg gtcgcaacca aacacccgaa
     1801 tgcggcacac gaaaccctcg ttcaactgga acgacggatt gaacactttt ggctcatcac
     1861 gcaaaacgtc gatggacttc atgccctggc cggctcgcaa cgtctttccg aaatccatgg
     1921 caacatctgg aaagtgcgct gtacgcaatg caaccggatt tcgccaaacc gcgacctgcc
     1981 tttggtctat ccacccatct gcacggactg ttcggggtta ttacgccctc atattgtgtg
     2041 gtttggagaa tcattggatc aggaagttat gaaccgcagt tatcaggcat tgcgaacttg
     2101 tgatattctt ctcattattg gcacgtcagg cgtggtctat ccggctgcat catttgcttc
     2161 gattgccaaa gacggtggcg cattagtggt cgaactgaat ttggacccga cgccaaactc
     2221 ctctgttgtg gacgtggcct ttcaaggccg tgccaaagac ctggtgcccc tgttgtgcga
     2281 agtcacctaa tcgcgccatt tcttgactcc cttcgtgggg gtttgttacg tttttttgtt
     2341 tagggattct cagtacttac gtttggagca cggtatgcaa ttgacgatca atggtaaacc
     2401 tgaaaccctg gaggtttcga cggtgctaga cgtcttgaaa accaaagata ttgacccgca
     2461 actcgtcgcg gtagaactga atacgcagat ggttgatcag gagcaactag gaaccacctc
     2521 cattaaagat ggcgataaac tagagttttt gtttttcatg ggcggtggcg cgtgagtgcc
     2581 ggatttcttc ggaatattac ggatggcatt gggcatactc cgttagtacg attaaaccgg
     2641 ctttcccctc aaggcggggc cacaatttat gggaaggcgg agttttacaa tcccggaggc
     2701 agcgtgaaag accgcatctg cctcaatatg attgatgagg cagagcaaaa gggactgctc
     2761 aagccgggtg gcaccatagt ggaaccgacc agcggcaata ccggcattgg attagccttg
     2821 gtgtctgccg ttcggggtta taaactgatt cttgtcatgc cggaaggcat gagcctggaa
     2881 cgggccagcc tgctctcctc atatggcgcg caattggtgt tgactcctgc tcgggaagga
     2941 atgcgtggat caattaaaga agcagaaagt attttggacc agaatcccga atatttcatg
     3001 ccgaaccagt tttctaatcc ggccaatcca gccatgcatc gcaagaccac ggctttggaa
     3061 atctgggaag ccttggatgg aaaggtggat gcattcgtgg ctgcagtggg caccggcgga
     3121 acgattaccg gctgtggtga actatttaaa gaaaaaaatc cctcggtcaa ggtgattgcg
     3181 ctagagccag ccggctctcc ggtcctgtcc ggaggagaac ccgggccaca cagaatccaa
     3241 gggatcggcg caggcttcgt ccccaaggtg ctgaatcgct cactcattga tgaaattatg
     3301 accgtgactg atgaagaggc ttaccaaacc acaaagcaac ttgccaggaa ggaagggctc
     3361 ctggtgggga tttctgcagg ggccaatgtg ttcgccgctc aaaaggtcgc ggaatcctta
     3421 gggccgaata agaatgtggt gacgattctc tgcgatacag gtgaacgata tctgagtatt
     3481 gagaaatatt tcaatattta ggggacttct actgaaccct ttccatgaag gaagcggcaa
     3541 gaggagaagg agcacccaac gtcccttccc caaacaagat aaggaaattt aagatatacc
     3601 agggcatatc ggctttctga gaaaaattct catggccaag aatcattt
//
LOCUS       DATIAL010000560        10140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1065930, whole genome shotgun sequence.
ACCESSION   DATIAL010000560 DATIAL010000000
VERSION     DATIAL010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065930"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /gene="gatB"
                     /locus_tag="VLA60_17110"
     CDS             <1..1434
                     /gene="gatB"
                     /locus_tag="VLA60_17110"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HSF11135.1"
                     /translation="VMAYEPVIGVEVHAQLQTKTKLFCGCPTSFGLPPNTQVCPTCLG
                     LPGSLPVLNRRAVEMAVRTGLALHCEIMLTNQFSRKNYLYPDLPKGYQISQFDLPICG
                     PGWLDISVNGESKRVRIRRAHLEEDAGKNVHDVPVEGSGVDLNRAGTPLLEIVTEPDL
                     HSSDEVDAYLKTLHDLLVYLDVCDGNMEQGSFRCEPNVSLRPRGQEALGTKVELKNIN
                     SFRFAKEALDFEISRQTEALTEGEAIRQETRLWDVQKGHTVVMRSKEEAHDYRYFPDP
                     DLLSVRVDAEWVESLRQTIPELPAARMKRFVTEYHLPEYDAKLLTDSKNLADYFEACV
                     KHFPHPKTVSNWVMGELLRELNTANTSPESSPVSPEQLVELLQLVEEGTISLKVARDL
                     FPEFYASGKTPEQLVNEKGLVQVSDEGVLEKLIEEVLAQHSAQVEQYRGGKETVLGFL
                     VGQVMKASQGKANPGKVNALLKQHLAS"
     gene            1507..2793
                     /gene="eno"
                     /locus_tag="VLA60_17115"
     CDS             1507..2793
                     /gene="eno"
                     /locus_tag="VLA60_17115"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HSF11136.1"
                     /translation="MSLIQNITSRMILDSRGNPTIEVDVHLASGVAGRAAVPSGASTG
                     TREALELRDGDKKQWMGKGVAKALAGIQKHMLPALRGMEAQDQVSVDSRLIKLDGTKG
                     KTRLGANATLGVSLAVARAAAQEQGIPLFRYIGGVSARELPVPMMNIINGGAHADNGL
                     DLQEFMIMPVGATSFSEALRMGTEIFHHLKAILKSKNMSTAVGDEGGFAPAVRSNEEA
                     LDLIVQAIQQAGYRSGKDVVLALDAAASEFFEKKMYHVRNGIKPKMSSGELVEYYAKL
                     VKQYPIVSIEDGLDESDWTGWKQLTDQLGDRVQLVGDDLFVTNVEFLGRGIREGIGNA
                     ILIKVNQIGTLTETIEAMRMAQRAGYGVVVSHRSGETEDTTIADLAVALNAGQIKTGS
                     LSRTDRLAKYNQLLRIEETLGSAALYRGKAVLPVQA"
     gene            2803..3156
                     /locus_tag="VLA60_17120"
     CDS             2803..3156
                     /locus_tag="VLA60_17120"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HSF11137.1"
                     /translation="MRPNNKKRASTGLGGDLLERLPMVGAFLMMSVLAGTMFFNSDGL
                     PLYFHMRETRQHLTEQIQQLQDINASIEEDIIRIQRDPQRLEELARNRLGMVRQGEVV
                     YQFVEPVPSSFTARP"
     gene            3245..4138
                     /gene="era"
                     /locus_tag="VLA60_17125"
     CDS             3245..4138
                     /gene="era"
                     /locus_tag="VLA60_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HSF11138.1"
                     /translation="MKSGQLAILGRPNVGKSTLLNALVREKIAIVSDKPQTTRNLILG
                     VGHYPEGQLILIDTPGLHTPHHRLNTQMVESALGTLTQSDVVYMIVDSQVLPGPGDRQ
                     VLEQIMSSHQERPFQGVFLLLNKADAVAKSRLLPLIDQYRTWFPWTEIIPISAKTGVN
                     IPQLVELTFACLPSCSELMYDEDFLTNQPMRHMAAELIREKVLAQTYDELPYAVGVLI
                     EEFQEEPGLTTIRAVILVEKTSQKGIVIGKKGMRLKEIGQSARLDMERLFGMKIYLRM
                     WVKVQEGWRDNVQVLNELGYG"
     gene            4202..4951
                     /gene="recO"
                     /locus_tag="VLA60_17130"
     CDS             4202..4951
                     /gene="recO"
                     /locus_tag="VLA60_17130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HSF11139.1"
                     /translation="MPLLRTRAIIIRSQRWGDADRIVTLYSADLGKIRGAARGARRMK
                     TRFGGVLEPFGIVDVTLFQKTPDALGQISQIDLVRNFKALREDLSVMAAAARMVHMVE
                     MITADRDPNPEMYSALVYALEHLLPDRDLALTTLLFQIHVLGYTGFRPQIDRCTECGN
                     IASTQMPQWFSPRLGGMVCHGCGQRGVGRLLSISKGSLAFIEQARRLPMASLSRLKAI
                     GTVRIEVEEAIEAYFQAVVGKALPTFEQWVS"
     gene            4980..5330
                     /locus_tag="VLA60_17135"
     CDS             4980..5330
                     /locus_tag="VLA60_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HSF11140.1"
                     /translation="MTSQATLEPTDMEWLENGVLGITWSDGHKGVYPVRYLRQHCPCA
                     ACTDEWTGELRLKPDSIPLLILLQDIEPVGRYAFRFKWSDGHDTGLYSYTALRRMCQC
                     EVCQPNKPRSKQLL"
     gene            complement(5475..6002)
                     /locus_tag="VLA60_17140"
     CDS             complement(5475..6002)
                     /locus_tag="VLA60_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11141.1"
                     /translation="MSNKSLVSSRIRYILVSLLSITVIGCQGLQPKSTGELAMEDHDF
                     LGIWDAYNHCVDSADIQHMQANLDVLASAPKPISLDDSPIPVPAFLKKWSTARGSRLA
                     VDPRAMAASCSIHLAEVAQLSADWPTALRTFQEIMKNYPEPQYAFYVSKANQAMEQLT
                     TVRPVSLSFQEALVD"
     gene            complement(6267..7322)
                     /locus_tag="VLA60_17145"
     CDS             complement(6267..7322)
                     /locus_tag="VLA60_17145"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HSF11142.1"
                     /translation="MHHDDLKRELQNLPVKYLHHMARGRIHRHFRLGKHRLVELMLAF
                     PPDQILAIETELQQILATRQERMAAQEARAAARPPIPASPFQAKNRPNKKRPTFGPFQ
                     PSITLKQILEGIGVPEPQPFVPDPWQTEAVEHLAHSDVIVSAPTGSGKTYVAIQAIRQ
                     AMALNQTVIYTSPLKALSNTKFMEFTQLFGPDQVGILTGDRRDNAQAPLLVMTTEILR
                     NLFYDAASGEIDLRLHTLGLVILDESQYLADPERGVVWEETIILCPSQARLLLLSASI
                     GNPQDLAEWLQAIRPIPCHLIRHAKRSVPLRGLYLHPTGQLVPLFKNQDILQGRGYTF
                     HPETKQLFAEYEIKPFH"
     gene            complement(7345..9204)
                     /locus_tag="VLA60_17150"
     CDS             complement(7345..9204)
                     /locus_tag="VLA60_17150"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11143.1"
                     /translation="MPPAIHPVDLIEILRRHRLTPAIVFLTSRRACDEAIDTFAHSSA
                     RMSTQRSEAIREFLEKFTKDFPSVEHHPLIPVVQEFGVAAHHAGHLPSWKIAIEELMR
                     QGLLDAVFATTTLAAGVDFPARTVVITQSSVRKSQDFTDLTNSEIQQLAGRGGRRGKD
                     LVGVVVITPSPYIDLNILGKGLTGYPEPIDSQFVVSYPMVLNLLKAHSLDQIEGLLAK
                     SFAQFQLNQRSSIHQDHLDQIKEQLTPYGPRECADWITQWKGFDLARRRKAHRHPNVT
                     NDTPFLTACLPFLTPGRLIGLPKGPAIILRQYRSRGTFAPMLSVIRAKGTLGECPAHS
                     VTQVYDRLFEFQPSRTIPWCQPQVLAQLKKELSQLPARLPTVPILPETQETDDPLLAE
                     VLTEEFPCPTCPSHPACKKDFPLASKLRQKSQQLTKTIQALRDGLWHRFQQKADVLHK
                     FGYITANSQLTADGEWARLIRINHSLLITELIRMEAFSGIAPGLLAGVMASISHDDDR
                     PGSYMRLPSGLASMLTQVRAVADSLSPYEPPPLLRSDVAALVESWIGNPQLTWASLVR
                     STSMAEGDVYRLLARTLEFLSQIYGLKVTHPGLADTAHSAMVTMRREVLQELP"
     gene            9378..9725
                     /locus_tag="VLA60_17155"
     CDS             9378..9725
                     /locus_tag="VLA60_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF11144.1"
                     /translation="MAIEVIIRGIRGATVVDFHGRLDMHSRWHVKAIINQCCHTEQEH
                     LILNLKGLTFVDSAGLGFLVLCSYQFRELRRRISWIQPQGCVGELLQNLQIHELISVC
                     QSEQDALSGVSPS"
BASE COUNT         2551 a         2249 c         2751 g         2589 t
ORIGIN      
        1 gttatggcct atgaaccagt tatcggagtt gaggtgcatg cccaactcca aacgaagacg
       61 aaattatttt gtggctgtcc gacctctttc ggtcttcctc cgaatactca ggtgtgtcca
      121 acctgtctgg gattacctgg aagtttgccg gtgctcaatc gacgtgcggt ggaaatggcc
      181 gtacgtacag gactcgcgct tcactgcgag atcatgctga ccaatcaatt ctcgcgaaaa
      241 aattacttgt accctgatct gccgaagggc tatcaaattt cgcaatttga tttgccgatc
      301 tgcggaccag gatggctgga catttccgtc aacggggagt caaaacgcgt gcgcattcgc
      361 cgggctcatt tggaagagga tgccgggaaa aatgttcacg atgtccctgt ggaaggcagt
      421 ggtgtcgatt taaatcgggc ggggaccccg ttattagaaa ttgtcaccga acccgacttg
      481 cattcgtccg atgaggttga tgcctatttg aaaacccttc acgatctttt ggtctacctg
      541 gatgtctgtg atgggaacat ggaacaaggg agttttcgtt gtgaaccgaa tgtgtcgctt
      601 cgtccacggg ggcaggaggc tcttgggacc aaagtcgaat tgaaaaatat caattccttc
      661 cgttttgcca aagaggcctt agattttgaa atctccaggc agacagaggc attaacggaa
      721 ggcgaagcga tccggcagga gacccggctg tgggatgttc agaaagggca tacggttgtc
      781 atgcgaagca aggaagaggc ccatgattac cgatattttc ccgatccgga cttactttcc
      841 gtccgggtgg atgcggagtg ggttgaatcg ttgcgtcaga ctattccgga acttccggct
      901 gcccgcatga agcgatttgt gacggaatat catctccctg agtacgatgc aaagcttctg
      961 accgattcaa agaatctggc cgactatttt gaggcctgcg tgaaacactt tccccatccg
     1021 aaaacggtca gtaactgggt catgggcgaa ttacttcgtg agctcaatac cgctaacacc
     1081 agtcccgaat cttccccggt aagtcccgaa caattggttg aacttttaca attggtggaa
     1141 gaggggacta ttagcttgaa agttgccaga gatctcttcc cggaatttta cgccagtggg
     1201 aaaactccgg agcaattggt gaatgagaaa ggcctggtcc aggtttccga tgagggtgtc
     1261 ctggaaaaac tgatagagga ggtgctggcc cagcattctg ctcaggtgga gcaatatcgt
     1321 ggcgggaagg aaaccgtatt aggttttttg gtgggccagg tgatgaaggc gtcacaggga
     1381 aaagccaatc ctggcaaggt caatgcgcta ttgaaacagc atcttgcatc ttgatctaag
     1441 actcatctca gggtacaaga ttaagacagg tgccattatt cacgatttaa aggaatgaca
     1501 gtttctatga gtcttattca gaacattacc tcacggatga ttctggattc tcgcgggaat
     1561 ccaacgattg aagtggatgt gcatcttgcc agtggcgtgg ctggtcgtgc cgctgttccc
     1621 tccggagcct ccacgggaac ccgtgaagcc ctggaactgc gggatggtga taaaaaacaa
     1681 tggatgggca agggtgtcgc taaggcctta gccgggattc agaagcatat gctcccggct
     1741 cttcgaggca tggaagccca ggaccaggtg agcgttgatt cccgattaat taaactggat
     1801 ggaaccaagg gcaagaccag attgggggcc aatgccacac ttggcgtctc gttagcggtg
     1861 gcgcgggctg cggcacagga gcagggaatt cccctctttc gctatattgg cggggtttcg
     1921 gctcgggaat tgccggtgcc tatgatgaat atcatcaatg gtggtgcgca tgcagataat
     1981 gggcttgatc tccaggaatt catgatcatg cccgttgggg caacgagttt cagcgaagcc
     2041 cttagaatgg gaacggagat tttccaccat ctgaaagcca tcttgaaatc caaaaacatg
     2101 agcacggcgg tcggggatga aggcggattt gcccctgccg ttcggtcgaa tgaagaagct
     2161 ctggatttaa ttgtccaagc gattcaacaa gcgggctatc gatcaggaaa agatgtggtg
     2221 ttggctcttg atgcggcggc cagcgagttt tttgagaaaa agatgtatca tgtgcggaat
     2281 gggattaaac ccaaaatgtc ttcaggagag ctggttgagt attatgccaa gttagtgaag
     2341 cagtatccga tcgtctcgat tgaagatggg cttgatgaaa gtgattggac tggatggaaa
     2401 cagcttacgg atcaactcgg ggatcgtgtc cagcttgtcg gtgatgactt gtttgtgacc
     2461 aatgtcgagt ttttgggacg agggattcgt gaaggcattg ggaatgctat cttgatcaaa
     2521 gtgaaccaaa tcggcacact cacggaaacg attgaagcga tgcgcatggc ccaacgagcc
     2581 ggctatggag tggtggtgtc tcaccgatcc ggtgagacgg aggatacgac cattgccgat
     2641 ttagctgtgg cgttaaacgc tgggcagatt aaaacaggtt ccctgtctcg taccgatcgt
     2701 ttggccaaat ataaccagtt actcaggatt gaggagacct taggctcagc tgccttatat
     2761 cgcgggaaag ccgttcttcc agtacaagca taattttctc gtatgcgtcc caataataaa
     2821 aaacgggcgt ccactgggct tgggggtgat ctgttggagc gccttccaat ggttggggct
     2881 tttttgatga tgagtgtgct cgcggggacc atgtttttta attctgatgg attgccttta
     2941 tattttcata tgcgagaaac gcgtcagcat ttgactgaac aaatccagca gttacaagac
     3001 atcaatgcct cgatcgaaga agatatcatc cgtattcaac gggacccgca acgactggaa
     3061 gaactggccc gaaatcgttt gggtatggtc cggcagggag aagtggtata tcaatttgtg
     3121 gagccggtcc cttcctcgtt tacggccaga ccttagcggc aagacttctt gcgccatacc
     3181 cctgtaaccc ttcatactca ttcattactg tttcttcggt tttcccatac ttgaaagctt
     3241 ttctatgaaa tcaggccaat tagccattct cggccgaccc aatgtcggca agtcgacgtt
     3301 gctcaacgca ttggtacgcg aaaagatcgc catcgtctct gataaaccac aaacaactcg
     3361 aaacctgatt ttaggggtcg ggcattaccc cgaaggtcag ctcattctca ttgatacgcc
     3421 tggcctccat acgcctcacc atcgcctgaa cacacagatg gtggaatcgg cgttagggac
     3481 cctgactcaa tccgatgtgg tctatatgat cgtggacagt caggtattgc cgggtcctgg
     3541 ggaccgacaa gtcctggagc agatcatgtc gtctcatcaa gagcgaccat ttcagggggt
     3601 atttttatta ttaaataaag ccgatgctgt cgccaaatcc aggctgctcc cccttattga
     3661 ccaataccgc acatggtttc cgtggacaga aattatcccc atttccgcca agaccggggt
     3721 aaatatcccg caattagtgg aattaacctt tgcgtgtctg ccctcatgtt cggaattaat
     3781 gtatgacgaa gattttttga ccaatcagcc tatgcgccat atggctgcgg agctcattcg
     3841 tgaaaaagtt ctagcccaga cgtatgatga attaccatat gctgtgggtg tcttgatcga
     3901 ggaatttcag gaagaaccgg gtctgacgac cattcgagcc gttattcttg tcgaaaaaac
     3961 aagtcaaaaa gggattgtga tcgggaaaaa aggcatgcgc ctcaaagaga ttggccaaag
     4021 tgcccggtta gatatggagc gactgttcgg catgaagatt tatctgcgga tgtgggtgaa
     4081 agtgcaggaa ggctggcgcg ataatgttca agtgttgaat gaactgggat atggatgaaa
     4141 aatttgtggt agcgacctca gtattcatgt ctgacctgta gaccgtactt gtagactact
     4201 catgccgctc ttacgaactc gtgcaatcat aattcgaagc cagcgatggg gtgatgctga
     4261 cagaattgtc actctatatt ccgccgattt gggaaaaatt cgaggcgcgg ctcgtggcgc
     4321 tcgccggatg aagacacgct tcggcggggt tcttgagcct tttgggattg ttgatgtcac
     4381 tctattccaa aaaacccctg acgctttggg gcagattagt caaattgatc tggtcagaaa
     4441 ctttaaagcc ttacgtgaag atttaagcgt gatggcagcg gctgccagga tggtacatat
     4501 ggtggagatg attacggccg atcgtgatcc caatccagag atgtattcag cactggtata
     4561 tgccctggaa cacttacttc ctgacaggga cctggccctc accaccttat tatttcaaat
     4621 tcatgtgctg ggctatactg gttttcgtcc acaaattgac cgttgtactg aatgcggaaa
     4681 tattgcctcc acccaaatgc ctcagtggtt ttcccctcga ttagggggga tggtatgcca
     4741 cggctgtggt caacggggtg tagggcggtt actgtctatt tctaaaggca gtttagcatt
     4801 tattgaacaa gctcgacgcc tacctatggc cagtctgtct cgattgaaag cgattggaac
     4861 ggttaggatt gaggtggaag aggcgattga agcctatttt caagcagtcg tcggaaaagc
     4921 tcttccaaca tttgaacaat gggtttccta gaatgcccca ctcataagga caatcaggta
     4981 tgacttctca agcaacactt gaaccgacgg atatggaatg gctagaaaac ggggttctgg
     5041 gcattacgtg gagtgatggg cataagggcg tctacccggt tcggtatttg cgacaacatt
     5101 gcccctgtgc ggcttgcacg gacgagtgga ccggggagtt gcgtcttaag ccagattcta
     5161 ttccgcttct catattatta caggatatag agccggttgg acgctatgcc ttccgattta
     5221 aatggagtga cgggcatgat accgggctat attcctatac ggcactgcgc cgcatgtgcc
     5281 aatgcgaggt ttgccagccc aataaaccca gaagcaagca acttctctag gttcttccgt
     5341 ttttctttgc ctcaggatcc cacgcaggaa atgccagtga tgtgttgaaa taaagcctca
     5401 aaagacgatt tttccaaaaa cgtcaacctg gagacctgga agaattgagt gaaaaaaata
     5461 gggatgggac gagtttagtc aaccagggct tcctggaaag aaagagacac aggtcgaacg
     5521 gtggttagtt gttccatcgc ctgattggct ttactgacgt agaaggcata ttgaggttcc
     5581 gggtaatttt tcatgatttc ctgaaatgtg cgcagtgccg ttggccaatc tgctgataat
     5641 tgggcgactt ctgcgagatg aattgagcag gaggcagcca tggctcgtgg atcgacagcc
     5701 agacgggaac ctctcgcggt agaccatttt tttagaaatg cagggaccgg aatgggagaa
     5761 tcgtccagtg agatcggttt cggtgctgag gctaacacgt caagattggc ttgcatgtgc
     5821 tggatgtcag cactatccac gcagtgatta taggcgtccc aaatcccaag gaaatcatga
     5881 tcctccatgg ccagttctcc agtggatttt ggctgaagac cctgacaacc tataaccgtg
     5941 atcgaaagta gactcaccag gatatatctg atacgtgatg aaacaagaga tttgttgctc
     6001 atattttaaa taattcctaa gaattcatat ttcacaaaaa aaggcaattt tatactaact
     6061 ctatgtggat aacgcaaaat ttatgctaaa aaacaattat taggaatgtg aattttttcc
     6121 cgtaaaatag caatattgac aatagattga gtgtcataac gtataataat taccacagcg
     6181 tgttgttttt ttgtcgcaag ccgcagtggc ttgaaaatta atgttttttg cattagttaa
     6241 tgattgataa atgcctttta cctgaattaa tggaatggtt taatttcgta ttcggcgaac
     6301 agttgcttcg tttccggatg aaatgtatag cctctgcctt ggagaatatc ttggtttttg
     6361 aatagcggaa caagttgtcc tgtaggatgc agatacagcc ctcgaagagg aacggatcgt
     6421 ttggcatggc ggataagatg gcagggaata ggcctgatgg cttgcagcca ttcagccaga
     6481 tcttgaggat tcccgatcga ggctgagaga agaagaagcc gtgcttggga tggacacaaa
     6541 ataatggttt cttcccacac caccccccgt tctggatcgg cgagatattg ggattcatcc
     6601 agaatgacca gacccagagt atgcagccgc aaatcgatct ccccgctggc ggcatcataa
     6661 aataaatttc tcaaaatctc ggtcgtcatg accaggagcg gggcctgggc gttgtcacgc
     6721 cggtctccgg ttaaaatccc gacttgatcc gggccaaaaa gctgggtgaa ttccataaat
     6781 tttgtatttg acagcgcttt tagtggagag gtgtaaatca ccgtttggtt gagtgccatg
     6841 gcctgtctga tcgcttgtat ggcgacgtag gtttttccgc ttcccgtcgg ggcgctaacg
     6901 atgacatctg aatgcgccag atgttccacc gcctcggttt gccagggatc cggaacaaag
     6961 ggttgaggct caggaacgcc aatcccttcc aagatttgct taagcgtaat cgatggctga
     7021 aaagggccga aggtgggcct ttttttattg gggcgatttt ttgcttgaaa gggcgaagca
     7081 ggaattggag ggcgtgctgc ggcgcgggct tcttgcgcgg ccatgcgttc ctggcgggtg
     7141 gccagtatct gctgcagttc ggtttcgatg gccagaatct ggtccggtgg aaaggccagc
     7201 attaattcaa caagtcgatg ttttccgaga cgaaaatgtc gatggatgcg gcctcttgcc
     7261 atgtgatgca aatattttac cggtaaattt tggagttcac gtttgagatc atcatgatgc
     7321 atgacattat attccaatct gtgcttatgg aagttcttgc aggacttccc ggcgcatcgt
     7381 caccatcgcg gagtgggcgg tatcggccaa gccgggatgt gtaaccttga gtccatatat
     7441 ttgagagaga aattccaagg ttctggctaa gaggcgatac acatctcctt cggccatgga
     7501 tgtggaacga accagtgaag cccaagtgag ttggggattc ccgatccaac tttccacgag
     7561 tgccgcgaca tctgagcgca acaagggcgg gggttcatac gggctcaagg aatcagcgac
     7621 ggcccgcacc tgtgtcaaca tggaggccag tccggacggg aggcgcatgt aggatcctgg
     7681 gcgatcatca tcatgggaaa tactcgccat cacgcctgct aacaatcctg gcgcaatacc
     7741 tgagaaggcc tccatacgaa tcagttctgt gatgagtagg gaatgattaa tacgaatgag
     7801 tctagcccat tccccgtcag ccgtgagttg cgagttggcg gtgatatatc cgaatttatg
     7861 gagtacgtcg gctttttgtt gaaaccggtg ccacaatcca tcacggagtg cttgaatcgt
     7921 tttggtcaat tgctgggatt tctggcgaag tttcgacgcc aggggaaagt cttttttgca
     7981 ggccgggtgg gagggacagg tgggacaggg aaattcctct gttaatactt ctgcaaggag
     8041 cggatcgtct gtttcctggg tttccggcaa aatcggaacg gtgggcagac gcgcaggcaa
     8101 ttgggagagc tctttcttga gttgagcgag tacctgtggc tgacaccagg gaattgtgcg
     8161 agagggctga aactcaaaaa ggcggtcgta gacctgagtg accgaatggg caggacactc
     8221 acccaaggtg cctttcgccc gaatcaccga taacattggg gcaaaggttc cacggcttcg
     8281 atactgacgg agaataatcg ccggaccttt tggcagtccg atgaggcgcc ctggagtcag
     8341 aaatggcagg caggccgtca agaagggcgt gtcgttggtt acgttagggt gccggtgcgc
     8401 tttgcggcgg cgggccagat caaagccctt ccattgagtg atccaatcgg cgcattcccg
     8461 gggtccgtat ggtgttaatt gttccttgat ctgatccaga tgatcctgat ggatactgga
     8521 tcgttgattc agctgaaatt gcgcaaagct ttttgcgagg agtccctcaa tctgatccag
     8581 ggaatgggcc ttcagcagat taagcaccat gggataggac accacaaatt gactgtcgat
     8641 tggttccgga tagcctgtga gtcctttgcc aaggatgttt aagtcaatgt aaggagaggg
     8701 agtaataact accacaccca ccaaatcttt gcctcgacgc ccaccacggc cggccaactg
     8761 ttgaatttcg ctgttggtta gatccgtgaa atcctgcgat ttacgtacgc tggattgggt
     8821 aataacaacg gttcgtgccg ggaagtcgac ccccgcggcc aaggtcgttg tggcaaacac
     8881 tgcatccaat aaaccctggc gcatgagttc ttcaatcgca attttccagg aagggagatg
     8941 ccctgcgtga tgagctgcga ctccgaattc ttgcacaaca ggaattaagg ggtgatgctc
     9001 aacactcggg aagtctttgg tgaatttctc cagaaactcc cgaatagcct ccgagcgttg
     9061 agtcgacatc ctggcagaac tgtgggcgaa ggtatcaatt gcctcatcgc aggcccggcg
     9121 ggaggtcaaa aagacaatcg ccggagtcag gcgatgccgg cggagtattt ctatgagatc
     9181 aacaggatga atggctggtg gcatggataa tgaaattgta gcaggagtcg gtgggtgaaa
     9241 acactaatat gatacaggtc gattaaaaga aaaggaacca tgctctttgg catcattatg
     9301 ggttgtgaaa gatgagcagc cagggagaaa cgttcgttaa ggtctgacgt attggaccga
     9361 taaggtttcg taagattatg gccattgaag tcattatacg tggaattcga ggcgccaccg
     9421 tcgtagattt tcatggtcgg ttagatatgc attcccgatg gcatgttaag gccattatca
     9481 atcaatgttg ccataccgaa caagagcatc tgattctcaa cctcaaaggc ttaacgtttg
     9541 tggatagcgc cggcttaggg tttctggtgt tgtgctccta tcaattcagg gaattacgtc
     9601 gcaggataag ttggattcaa ccacaaggat gtgttgggga attgcttcaa aatctccaaa
     9661 tccatgaact catctcggtg tgccagtccg aacaggacgc cttatctgga gtctctccct
     9721 cctaaatttg gctcattctg agaattcatt acatgatgtt ggtgcgtgaa tccatgggat
     9781 actaccaccg aagatttcgc aagaggcact ccgttcccgg atagtcaaga cattctcctc
     9841 ctctttactt tccaaaggtc ccccccaaca caactgagtg ttttgtatca atcgaagtcg
     9901 ttctctgcgt aagcatggtg agaatcatcc aaaatatacc gaacgactat ccggtatcct
     9961 ttggaaagaa cgagagtgcc ttgcccttgc cagcatagcc tataaaacgg caagatgatt
    10021 ttttgtgagg agtcttaagg ggtatggatg aatcaagaga tgcttcgttt taacatcccc
    10081 aaacgcacca tttcgtggag tgcatcgatg gcgaagggta tagtgaccga agggcggaaa
//
LOCUS       DATIAL010000561         4583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_851922, whole genome shotgun sequence.
ACCESSION   DATIAL010000561 DATIAL010000000
VERSION     DATIAL010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851922"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..718)
                     /locus_tag="VLA60_17160"
     CDS             complement(5..718)
                     /locus_tag="VLA60_17160"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HSF11145.1"
                     /translation="MARLGVNIDHVATLRQARGGHEPDPVMAASLVHLAGADGIVVHL
                     REDRRHIQDRDLTVLREIVQTHLNLEMAAEDSIAKIALNIKPDLVTLVPERRQELTTE
                     GGLEVTEHRDRIQAMVYLLHEGGIPVSLFIDPDVNQIRAAHKVHADSVELHTGRYANT
                     RRYQEEEAEFDTLLQAAKLATKLGLRVSAGHGLNYRNVKRLTGIFEIEEFNIGHSIIA
                     HAVFVGLERAVRDMKILLS"
     gene            1009..2154
                     /locus_tag="VLA60_17165"
     CDS             1009..2154
                     /locus_tag="VLA60_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF11146.1"
                     /translation="MFSQVQELKLQARKRGEDIIDLGMGNPDQPTPRHIVDKLVETAR
                     LGRNHRYSASRGITKLRHAICDWYQRNYEVSLDPETEAIVTLGVKEGLAHLALAMVGP
                     GDVVLAPTPTYPIHMYSVIIAGGEVRGVELGPDSDFFDNLTKAFKQTQPSPRALIISF
                     PHNPTTRVVDLEFFKKVVEFALEHQVWVIHDLAYADIVFDGYRAPSLLQIPEAKQIGV
                     EFYSLSKSYNMPGWRVGFCVGNREVIGALTKIKSYLDYGMFQPIQIASIIALNGPQDC
                     VKEIVGRYERRRNALVRGLNRVGWPIDYPRATMFAWAKIPQPYLGMGSLEFSKLLLRD
                     AKVAVSPGIGFGAGGDDYVRFALVENEHRIFQAIRGIRQALKLDGDL"
     gene            2154..3464
                     /locus_tag="VLA60_17170"
     CDS             2154..3464
                     /locus_tag="VLA60_17170"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HSF11147.1"
                     /translation="MRKAIRVGLIGFGTVGTGVVKILQGNADLITKRVGVPVSLVRIA
                     DLDVTTDRGISVPQGVLTTDVRDILTDPSIDIVVELIGGHEPAKRFILQALESKKCVV
                     TANKALLADHGEEVFEAAFKAGVDVGFEASVGGGIPIIRSLKEGLAANRILSITGIMN
                     GTSNYILTRMTNEHRDFCEILQEAKREGYAEADPSLDVDGVDAAHKLAIMVNLAYGTP
                     VPMSAIYTEGISHISTVDIEFARELGFTIKLLGIAKLQNNAIEARVHPALVPAGSPIA
                     QVNGVYNAIHLIGDAVGDIVLYGKGAGSLPTASAVVGDIIDLARNQIAGVASRVPVTS
                     FQWESRVPMAIMPMSDIESRYYLRCMVKDQPGVLSRISGILGQRGISISSVLQKGRKE
                     GQTVPLVILTHRSLERAVQSALEEINGLPMVSEPTTLLRMEGVE"
     gene            3503..4567
                     /gene="thrC"
                     /locus_tag="VLA60_17175"
     CDS             3503..4567
                     /gene="thrC"
                     /locus_tag="VLA60_17175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HSF11148.1"
                     /translation="MVIPWRGIIDEYRKFLPVGDQTPIVTLLEGNTPLISAKRLAKKV
                     CPGLDLYLKVEGANPTGSFKDRGMTMAVSKAVESKARGLMCASTGNTSASAAAYGAKA
                     GLPVYVVVPAGNIALGKLTQALMHGARVIQIEGNFDQALTIVKDLCQETAIELVNSIN
                     PFRLEGQKTAAMEICDQLGYAPVIHALPVGNAGNITAYWRGYREYQQAGQTQGAPRMF
                     GFQAEGAAPLIRGHIVESPKTIASAIRIGNPASWCFALQAVEESHGYLDMVSDEEILK
                     AYQMVAREEGVFCEPASAASLAGIEKMNRMGLLPKEGVIVCTLTGHGLKDPDTAISVS
                     YRPVTVPATHDAVRAQLTNP"
BASE COUNT         1103 a         1044 c         1270 g         1166 t
ORIGIN      
        1 gtcgttagga caggaggatt ttcatatccc ggactgcacg ttcaagaccg acaaatacgg
       61 catgagcaat aatactatgc ccaatgttga attcctcaat ttcaaaaatg ccggttagcc
      121 gcttgacatt ccggtaattc agcccatgtc cggcgctcac gcgtaatccc agtttggtcg
      181 cgagtttggc tgcttgaagc agcgtatcaa actcggcctc ttcttcttgg taacggcgag
      241 tattggcata ccggccggta tggagttcca cagaatcggc gtggacttta tgggcggccc
      301 gtatttgatt gacatccgga tcaataaaca ggcttacggg aatgccgcct tcatggagta
      361 agtacaccat agcctggata cggtcccggt gctctgttac ctccagtcca ccctctgttg
      421 tgagttcctg ccggcgttcg ggtaccagcg tgaccagatc gggtttaata ttcagggcaa
      481 ttttggctat tgaatcttcc gctgccattt cgaggttgag atgagtctgc acaatttcgc
      541 gcaacacggt cagatcacga tcctgaatat gccgccgatc ctcccggagg tgcaccacaa
      601 tgccatcggc tccggcgagg tgtaccaaag aggcggccat aacaggatcc ggttcatgtc
      661 cgccacgggc ttggcgaagt gtcgccacat ggtcgatatt cacaccgagc cgcgccatgg
      721 agttcccttc gaatcggagt aaaagtgttg aaccttcgag agttcattat ggcaaaaggg
      781 aaaatcctcc tgcaataaat gaaattctga tgaggaatgg aatgcgctcg aggattgttt
      841 aaaaaatcca tggtcatgaa attgactggt ctcgtcccct catctagaat aaaaactcta
      901 ttaaatcgta cgattttcta tcatttcctt ccccaaccat caaggacgat tcgtcatttt
      961 gcactaacaa tggaaccttt ttaccgaatt caacgattgc caggatacgt gttttctcag
     1021 gtccaggagt taaagctcca agcccgaaag cgaggagaag atattattga tttagggatg
     1081 ggcaatcccg atcagcccac accccgtcat atcgtggaca aattggttga gacggcgcga
     1141 ctcggaagga atcatcgtta ttcagcttca cgcggtatta ccaaattacg ccatgcgatc
     1201 tgtgattggt atcaacggaa ttacgaggtg agcttagatc cggaaacgga agccattgtg
     1261 accttgggag tgaaagaagg gttagcacat ttggccctgg ccatggtggg accgggtgat
     1321 gtcgtcctgg cgccgacacc cacctatccg attcacatgt acagtgtgat tattgcgggt
     1381 ggcgaagtgc ggggagttga attaggtcct gattcggatt tttttgacaa cctgacaaag
     1441 gctttcaagc aaacgcaacc gtccccgcga gcattaatta tcagttttcc ccacaatccc
     1501 accacccgtg tggttgacct ggagttcttt aaaaaagtgg tggaattcgc cctggagcac
     1561 caggtttggg tcattcatga tctggcgtat gccgatattg tgtttgatgg atatcgtgcc
     1621 cccagcctac ttcaaattcc ggaggctaaa caaatcgggg tggaatttta ctccctttcg
     1681 aaaagttata atatgccggg ttggcgggtg ggattttgtg tcgggaatcg tgaagtcatt
     1741 ggcgcattga ccaagatcaa aagttatctg gattatggaa tgttccagcc cattcaaatc
     1801 gccagcatta ttgccctaaa cggtccgcag gattgcgtga aagaaattgt tggacgatat
     1861 gagcgacggc gtaatgcgct tgtccgggga ctcaatcgcg tcgggtggcc cattgattat
     1921 ccacgcgcca ccatgttcgc ctgggcaaaa atccctcaac cctatctcgg tatgggatca
     1981 ttggagtttt ccaagcttct gttgcgggac gccaaagtgg cggtttcacc tggaatagga
     2041 tttggcgcag gtggcgatga ctatgtccgt tttgccttag tcgaaaatga acaccggatt
     2101 tttcaggcga ttcggggaat ccgtcaagcg ttaaaattgg acggggatct ttaatgagaa
     2161 aagccattcg ggtcgggctg atcgggtttg gtacggtcgg aacgggggtc gtgaagattc
     2221 tccaagggaa tgccgacctc attacgaaac gggttggggt tccggtgtcg ctcgttcgga
     2281 ttgcagattt ggatgtgaca accgatcgcg gcatatcggt tcctcaagga gtcttgacga
     2341 cggacgtccg ggacattttg actgatcctt ctatcgatat cgtggtggaa ttgattggcg
     2401 ggcatgaacc cgccaaacga ttcattcttc aggcccttga atcaaagaaa tgcgtggtga
     2461 cggcgaataa agctttattg gccgatcatg gggaggaagt gtttgaggcg gcatttaagg
     2521 ctggcgtgga tgtgggtttt gaagcgagcg tgggaggagg aattcccatt atccgatcgc
     2581 tgaaggaagg gttggccgca aaccggatac tgtccatcac gggcatcatg aatggaacat
     2641 ccaattatat tctcaccaga atgacgaatg agcaccgtga tttttgtgaa attttacaag
     2701 aagctaaacg ggaggggtat gccgaagccg atccctctct ggatgtcgat ggggttgatg
     2761 ccgcccataa attggcgatt atggtgaatc ttgcctatgg cactcccgtg cccatgagtg
     2821 ccatttatac ggaaggaatt tcgcatattt cgacggtgga tattgagttt gcccgtgaac
     2881 tgggatttac gattaaattg ctgggaatcg ccaaattaca aaataacgcc attgaggcac
     2941 gggtgcatcc cgccctagtg ccggccggtt ctcccattgc ccaggtcaat ggggtctata
     3001 atgcgattca tctgataggt gatgccgttg gagatattgt gttgtatgga aaaggtgccg
     3061 ggtctcttcc aacggccagt gcggttgtgg gcgatattat cgatctggcg cgcaatcaaa
     3121 tcgcaggggt tgccagccgg gttcccgtga cgtcttttca gtgggagtct cgtgtcccaa
     3181 tggccattat gccgatgtcg gatattgaaa gccggtatta tttgcggtgc atggtcaagg
     3241 atcaacccgg agtattatcc aggatatcgg gaatccttgg tcagcgtggg atcagcattt
     3301 cgtctgtcct tcaaaaagga cgaaaggaag gacaaactgt gccattggtt atcttgacgc
     3361 atcgatcatt ggagcgggcg gttcaatcgg cactcgagga aatcaacggg cttcctatgg
     3421 tgtctgagcc gacgacatta ctacgtatgg aaggggtgga ataacacatg gagccatatg
     3481 gcttcacgaa gggtggcgac tcatggtgat accttggcgt ggaattatcg atgaataccg
     3541 gaaatttctc cctgtgggag atcagactcc gattgtgact cttctggagg gaaatactcc
     3601 actgatctcc gcgaaacgct tagccaagaa agtttgcccg gggttggatc tgtatttgaa
     3661 ggtggagggt gccaatccaa ccgggtcctt taaggatcgg ggtatgacca tggcggtgtc
     3721 caaagccgtg gaaagcaagg ctaggggact aatgtgtgcc tctaccggca atacctccgc
     3781 ttcggcagca gcctatgggg caaaagcggg attgccggtg tatgttgtcg tgcccgcagg
     3841 caatatcgcg ttgggtaagt tgactcaggc tctcatgcat ggagcgcgag tgattcagat
     3901 tgaagggaat tttgatcaag ccttaaccat cgtgaaagat ttatgtcaag aaacggccat
     3961 tgagttagtg aattcgatta atccgtttcg gttggaaggt cagaaaacgg cggcaatgga
     4021 aatctgtgat cagttgggtt atgcccccgt gattcatgct cttccggtag ggaatgccgg
     4081 gaatattact gcatactggc gtggatatcg ggagtatcaa caagcgggac aaactcaagg
     4141 agcgcctcgg atgttcggct ttcaagctga aggagccgct cctctgatca gaggccatat
     4201 cgtggagtcg ccgaaaacca ttgcctcagc cattcgaatc ggcaatcctg ccagttggtg
     4261 ttttgccctg caggcagtgg aggaatcaca cggatacttg gatatggttt ctgatgaaga
     4321 gattctcaaa gcctaccaaa tggtggcgcg tgaggaaggc gtcttttgtg agcctgcttc
     4381 cgctgcgtct ctcgcaggga ttgaaaaaat gaaccgcatg gggctgttgc cgaaagaagg
     4441 cgtcattgtc tgtaccttaa ccgggcatgg gttgaaagat cctgatacgg ccatatcggt
     4501 atcatatcgc cctgtgactg ttcctgcgac acatgacgcg gtcagggcgc aattaacaaa
     4561 cccctaatcc ctcattaaca atc
//
LOCUS       DATIAL010000562         3890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1498356, whole genome shotgun sequence.
ACCESSION   DATIAL010000562 DATIAL010000000
VERSION     DATIAL010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498356"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VLA60_17180"
     CDS             <1..1533
                     /locus_tag="VLA60_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HSF11149.1"
                     /translation="NRTVTFLREQLGIQRGDRIATLLFNHDFTVVMYYAAWVLGAVMV
                     PIDLNESEDRRFYILEHSEACVVFCWGTEYEALQDIRNRLPHLRHLVSLGNGTSPLMD
                     MMGQPRPAQSYNHGSLDQAALQDDALIVYTSGTTGPPKGVVLTIENLLVDAHGIARWH
                     GLEKGHCLMCVLPIHHVNGIVVTLITPFYGQGRSVLNRRFRQSAFWNRIRDEGVTCVS
                     VVPTILEFLLEGARDNGQYRLDHFRGLICGAGPLLKDTVSRFEHQFHAPVRHGYGLSE
                     TTCYASFLPVNLSEQEHRHWLTDYDYPSIGLPIPHNDMAILNPQGQPLGEMEKGEICI
                     RGQTVCSGYLKRPDANEESFRWGWFRSGDEGFFVRDQGDRPYFFISGRLKELIIRGGV
                     NISPLEIDEVLRRHPSVKFGMAVSFENRFYGEEIAGYLVVKEHMPQPSEDEMVTWCRQ
                     ALPYAKCPKVIMFGQEMPYTTTGKPKRLELKARLSPTLARYRDVQFRSLQRGSTQKDQ
                     MA"
     gene            1637..2620
                     /locus_tag="VLA60_17185"
     CDS             1637..2620
                     /locus_tag="VLA60_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HSF11150.1"
                     /translation="MEISQSEFLHLYYFLKLTRALEERVTTLYRQGRIVGGAYTSHGM
                     EAISVGYASALQADDIIAPFHRDMGAFLVRGITPGEVLAQYLGKRTGPTKGKDGNVHM
                     GDLKRGIIAFVSHLADNLPVATGVALAFKLRGESRIVAANTGDGGTSRGDFHEALNFA
                     SVRRLPVVFFCNNNQYAYSTPLHHQMAIKDVVERAKAYAIPGEIVDGNDVASVYLTAQ
                     RAFQRARNGGGPTFIECKTMRMHGHSEHDSAKYVPRELLEEWKKKDPILKAERRLKEL
                     GYAGQPEFQEIHMRVEKEIESALEFAEQSPLPEGPEALEGVFAANPVEVLI"
     gene            2617..3609
                     /locus_tag="VLA60_17190"
     CDS             2617..3609
                     /locus_tag="VLA60_17190"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HSF11151.1"
                     /translation="MTTSAITHEVSYLEAISQALDEEMSQDERVFLMGEDIGPYGGAF
                     RVTEGFQDKYGEWRVLDTPLAESGIVGAAIGAAMMGMRPVVEMQFADFISCAFDQITE
                     MAAKNHYRWGAEVPLVIRAPFGGGTHGGPFHSVCPEAWFFHTPGLKLVAPSTAYDAKG
                     LLKAAIRDHNPVIYFEHKFLYRRVKEMLPAEDYIVPIGEADIKRTGSEISVITYGAMV
                     HVALEAAEALDQEGIDMEVVDLRTLSPLDTDTVKNSVRKTGKVIILHEDTKTGGIGGE
                     IAAILAEECFNALDAPIMRIAAPDIPVPYSPPLEDFFLPKAHDVITAARQLAAY"
     gene            3629..>3890
                     /locus_tag="VLA60_17195"
     CDS             3629..>3890
                     /locus_tag="VLA60_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HSF11152.1"
                     /translation="MATDIVMPQLGESIAEGTIVKWLVPLGAQVKKDESLLEVETDKV
                     ALDIPSPATGALTEILIQEGETVPVGTLLARLDYHSESGNGDS"
BASE COUNT          918 a         1000 c         1075 g          897 t
ORIGIN      
        1 aatcgaaccg taaccttcct gcgtgagcaa ctggggatcc aacggggtga tcgcattgcc
       61 accctgctgt ttaatcatga ctttaccgtg gtgatgtatt atgccgcatg ggtcctggga
      121 gccgtgatgg tgccgattga cctcaatgaa tctgaagacc gtcgcttcta tattctggaa
      181 cattcagagg cctgtgtggt tttttgttgg ggaaccgaat acgaagccct ccaggatatc
      241 cggaatcgac tgccccacct ccggcatctc gtctctctcg ggaatggaac ctcaccgctc
      301 atggacatga tgggtcaacc tcgcccagct caatcataca accacggatc tctcgatcaa
      361 gctgctctcc aggatgacgc attgattgtc tacacctccg gaacgacagg accgccgaaa
      421 ggggttgtgc tcacgattga aaatcttctc gtcgatgccc acggcattgc cagatggcac
      481 ggattggaaa aaggtcattg cctcatgtgt gtgctgccga tccatcatgt aaacggaatt
      541 gttgtcacac tcatcacacc attttatgga cagggcagat ccgtgctgaa ccggcgtttt
      601 cgtcagtccg cgttttggaa ccgaatccgg gatgaagggg ttacctgcgt gagtgtggtc
      661 cctaccattc tggagttcct gctggaagga gcccgggata acgggcaata ccggcttgac
      721 cattttcgtg gattgatttg tggtgccggc cctctcttaa aggacacggt gtcacggttt
      781 gagcatcaat ttcatgctcc ggttcgtcat gggtacggac tatctgaaac cacgtgttat
      841 gccagttttc tccccgttaa cctttccgaa caggaacaca ggcattggct tacggactat
      901 gactacccat ccattggcct accgatccct cacaacgaca tggcgattct caatccccag
      961 ggacaaccgc tcggcgagat ggaaaagggg gaaatttgta tccgcggaca aacggtgtgt
     1021 tcgggttact tgaaacgacc ggatgcgaat gaggaaagtt tccggtgggg atggttccgg
     1081 tcgggagatg aggggttctt cgttcgtgat cagggtgatc gcccgtactt tttcatatcc
     1141 gggcgactga aagaattaat catccgtggc ggtgtgaata tttcgccctt ggaaattgac
     1201 gaagtgctca gaaggcaccc ttccgtcaaa ttcggtatgg ccgtttcctt tgaaaaccgt
     1261 ttctacggtg aagaaattgc cggatacctc gttgtcaaag aacatatgcc gcaaccttcg
     1321 gaagatgaga tggtcacatg gtgtcgccag gcgttgccct atgccaagtg ccccaaggtc
     1381 atcatgtttg gccaggaaat gccctatacg acaacgggga aacccaaacg tttggagtta
     1441 aaggcccggt tgagtcccac attggcacgc taccgcgacg tccaattccg ctctttgcaa
     1501 cgcggctcaa ctcaaaaaga tcagatggct tgaggaggtc gtcattgaat gagtagtgga
     1561 tatgacgtat cacccatacc acacccaatg accaacaaga ggataattat tcatgtctag
     1621 acagaaggag agtgttatgg agatttcaca atctgaattt ctccacctct attatttcct
     1681 taaactcacc agagcgttgg aggaacgagt gaccacgctg taccggcaag gtcgcatcgt
     1741 cggaggtgcg tacaccagcc atggaatgga agcgatttcg gtgggctatg cttccgccct
     1801 gcaggcggat gacatcatcg cccccttcca tcgggatatg ggcgcgttct tagtcagagg
     1861 cattacgccg ggtgaggtct tagcgcaata tcttggaaag cgcaccggac ccaccaaagg
     1921 gaaggacggc aacgtgcata tgggcgacct gaaacgtggc atcatcgcct ttgtcagtca
     1981 tctggctgac aatcttcccg tcgcgaccgg ggtggctctg gcgtttaagc tccgtggtga
     2041 atcccgcatt gtcgccgcga atacaggaga tggagggacc agtcgcggag attttcatga
     2101 agcgttgaac ttcgcgtccg ttcgccggct gccggtggtg ttcttttgca ataacaacca
     2161 atacgcctac tccacccctc ttcaccatca gatggccatc aaagatgtcg tcgagcgggc
     2221 aaaagcctat gccatccccg gtgaaattgt ggacggtaac gatgtcgcct ccgtctacct
     2281 gaccgcacaa cgggcctttc aacgtgccag gaacggaggg ggtcccacct ttatcgaatg
     2341 caaaaccatg cgtatgcacg ggcattcgga acatgattcc gccaaatatg tgcctcggga
     2401 acttctggag gagtggaaga agaaagaccc cattctgaag gcggaacgcc ggctgaagga
     2461 attggggtat gccggtcaac ccgaattcca ggaaattcat atgagggttg aaaaggaaat
     2521 tgaatccgcc ttggagttcg cggaacaaag cccgcttccg gaaggtccgg aagctttgga
     2581 aggcgtcttt gcggccaatc ccgtggaggt actgatatga ccacatccgc catcacacat
     2641 gaagtgagct atctggaagc catttcgcaa gccctggatg aagaaatgag ccaggatgaa
     2701 cgggtcttcc tgatgggaga ggatatcggc ccttacggcg gggcatttcg tgtcaccgaa
     2761 gggttccaag ataagtatgg tgaatggaga gtgctggaca cccctctggc ggaatcggga
     2821 attgtcggcg ccgcgatcgg agcggcgatg atgggcatga gacccgtggt ggaaatgcaa
     2881 tttgcggatt tcatttcctg tgcgttcgat caaattaccg agatggccgc caagaatcat
     2941 tatcgctggg gagcagaggt gcctctggtc attcgcgcac ccttcggggg aggtactcat
     3001 ggagggccgt tccattccgt gtgcccggaa gcctggttct ttcatacccc cgggttgaaa
     3061 ctggtggcgc cctcaaccgc ctatgacgcc aaaggattgt tgaaagccgc tatccgtgat
     3121 cataaccccg tcatttattt tgagcataaa tttctgtatc ggcgcgtgaa ggagatgctt
     3181 ccggctgaag attatatcgt cccgatcgga gaagcggaca tcaaacggac aggaagcgag
     3241 atatccgtca tcacctatgg agccatggtc cacgtggcat tagaggcagc agaggccttg
     3301 gatcaggagg ggatcgacat ggaagtcgtg gatcttcgca ctctgagccc tcttgatacc
     3361 gacacggtca aaaactctgt tcgaaaaacg ggcaaagtga ttattctcca cgaagatacg
     3421 aagaccggag gaatcggtgg agagattgcc gcgattctgg ctgaagaatg cttcaacgcc
     3481 cttgatgcgc cgattatgcg gatcgcagcc cccgatattc ccgtgcccta tagtcccccg
     3541 ctggaggact tttttcttcc gaaagctcat gacgtcatca ccgccgcccg ccaattggcg
     3601 gcctattgat tacacagggg agcaattcat ggccaccgac atcgtcatgc cgcagttagg
     3661 tgaaagcatt gccgaaggca ccatcgtgaa atggctcgtt ccgctggggg cacaggtgaa
     3721 aaaagatgaa tccctgttag aggtggaaac cgataaggtc gcattggata ttccttcccc
     3781 ggcgaccgga gccttaaccg agattttgat tcaggaaggg gaaacggttc cggtgggcac
     3841 ccttcttgcg cgactcgatt accattccga atccggaaac ggtgattccc
//
LOCUS       DATIAL010000563         5434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_529423, whole genome shotgun sequence.
ACCESSION   DATIAL010000563 DATIAL010000000
VERSION     DATIAL010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529423"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..368)
                     /locus_tag="VLA60_17200"
     CDS             complement(54..368)
                     /locus_tag="VLA60_17200"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HSF11153.1"
                     /translation="MATNHSGASAGSVALAFLSGALLGAVTAIMLAPQPGRDSRERIT
                     GYARRTSEDLRDIKERATDAVEDVVGRGRELVEEISCAVREALDAGRDAMRREREMKS
                     LD"
     gene            complement(358..765)
                     /locus_tag="VLA60_17205"
     CDS             complement(358..765)
                     /locus_tag="VLA60_17205"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HSF11154.1"
                     /translation="MVDLAAIIIAVAFVVLVGYLVPMILQVKRTVGQSERLLIRLNHE
                     LPGLLKDVKGTNENILALTDQARLGMDRATIFLNAVGDVGETVNHVHQTVRGKGGALA
                     VGVSSLLAGLKAASSTMRKRVHKEQKGGPSYGN"
     gene            complement(780..2252)
                     /gene="gatA"
                     /locus_tag="VLA60_17210"
     CDS             complement(780..2252)
                     /gene="gatA"
                     /locus_tag="VLA60_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HSF11155.1"
                     /translation="MSLFKLTLKELQGKFTAGEISAVDIARSYSLRLSQVEPKIKAYI
                     TLTPKETLLQQAKAIDDGLKAWRKTQPLMGMPLGIKDNICTEGIRTTCGSRMLENFVP
                     PYDATVMGRLRNHQPLILGKTNLDEFAMGSSTEHSAFGPSRNPWNQKFIPGGSSGGSA
                     AAVAADECVAALGSDTGGSIRQPAACCGVVGLKPTYGRVSRFGLVAFASSLDQIGPIT
                     KTVTDAAILLNAIAGFDPMDSTSANRDCPDFTKAVKKKDIKKLIIGVPREYFSEGLDT
                     EVYEKVQDAIQVLEELGGTIREVTLPSTERAIATYYLIATAEASSNLARYDGVRFGFR
                     AKESRELAEMYRSTRSQGFGPEVKRRIMLGTYALSAGYYEAYYGKAQAVRTLIQQEFD
                     AVFQEVDLLVSPVMPTPAFQLGERLDDPLQMYLSDIYTIPASLSGLPAISVPCGLSKM
                     GLPIGLQLMGRPFEEAMVLRAAYAYEQATDWRMKRPVIRS"
     gene            complement(2340..2687)
                     /gene="panD"
                     /locus_tag="VLA60_17215"
     CDS             complement(2340..2687)
                     /gene="panD"
                     /locus_tag="VLA60_17215"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HSF11156.1"
                     /translation="MYRQLLRCKIHRATVTGSSLEYEGSLTIDEDLMERAGIMSYEAV
                     MVSNLNNGERFSTYALPGTRGKGEIILNGPTARLGVIGDKVIIFCYELFNYEEAKRHV
                     PQIIQVDHQNKAK"
     gene            complement(2752..3045)
                     /gene="gatC"
                     /locus_tag="VLA60_17220"
     CDS             complement(2752..3045)
                     /gene="gatC"
                     /locus_tag="VLA60_17220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HSF11157.1"
                     /translation="MASITQKEVEHVAQLARLDLSEAEKPMFADQLNHILSYVDQLQG
                     VSTEGVPPTASVAHEEQVFREDSPRECLSVEKALANAPESHNGFFVVPNILGK"
     gene            complement(3226..3948)
                     /gene="radC"
                     /locus_tag="VLA60_17225"
     CDS             complement(3226..3948)
                     /gene="radC"
                     /locus_tag="VLA60_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HSF11158.1"
                     /translation="MNDPQLKKGIQSWPEKERPRELLLNNGAQGLSDAQLVAILLRIG
                     RPDSSAVDVALDLLTRLKGLRGLANRSLQELCVVPGIGPAKAAQILAAVELGKRALAV
                     PLTAGLRIHHSHDIYQHYYPLLRDLRHEVFKALLLDAKHGLIRDVTVSEGSLTVSIVH
                     PREVFNLAVRESAAAVIFVHNHPSGDPHPSEEDHALTRRLMAAGEILGIRVLDHIVIG
                     DGRYVSFADEGFLFSPTPTLDH"
     gene            4086..4682
                     /gene="rsmD"
                     /locus_tag="VLA60_17230"
     CDS             4086..4682
                     /gene="rsmD"
                     /locus_tag="VLA60_17230"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HSF11159.1"
                     /translation="MKPSSIRIVAGQFKGRTIPTLPGRTTRPTSQRAREALFSIMGGQ
                     IQDAIVADLFAGTGAVGLEALSRGATKALFVEQNPLAGTTIQQMLTQLKVTPSSQVLI
                     EDVSLAIQNSILVGWRPFDMIFMDPPYRLPNVQQILHQLEEADVMAPTGRIIYEHFHK
                     TTPPIPLGKWSLIRTARYGDTALTFYRPVPPTVKELDQ"
     gene            4679..5167
                     /gene="coaD"
                     /locus_tag="VLA60_17235"
     CDS             4679..5167
                     /gene="coaD"
                     /locus_tag="VLA60_17235"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HSF11160.1"
                     /translation="MTIGIYPGTFDPITRGHTDVIARSLRLVEQVIVAVAPNPRKAPL
                     FDLDERVQLAKIATSDLKGVTVEPFDGLLVNYVRKRGALAIIRGLRAVSDFEHEFQMA
                     LLNRKLDSSLETVFLMSSEEFSYLTSSMVKEVASVGGPLHHFLHPEVAEWLQGRLRRN
                     AK"
     gene            5164..>5434
                     /locus_tag="VLA60_17240"
     CDS             5164..>5434
                     /locus_tag="VLA60_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF11161.1"
                     /translation="MNLATRTTRITPSPTLQLSATVKALVAQGQQIFDFTAGEPSLDS
                     PEEAKEAAYQAIRSGFTKYTAVTGIDDLKEAIIEKFQRDQGLTYSR"
BASE COUNT         1388 a         1487 c         1235 g         1324 t
ORIGIN      
        1 tcataggcca taacgcagca gagccactcc tctcgatttc gtcgtttttt atcttaatcc
       61 agtgatttca tttcacgttc acggcgcatg gcatcccgac ccgcgtctaa ggcttctcgc
      121 acggcacagg aaatttcttc caccagttca cgtccacgac caacgacatc ttctaccgca
      181 tcagtggccc gctccttaat gtctcgcaaa tcttcactcg ttcgccgagc atagcccgtg
      241 attcgttctc gggaatctct tcccggctga ggagctaaca tgatcgcggt cacagctccc
      301 aatagcgctc cacttaaaaa tgccaacgca accgatcctg ctgatgcacc tgaatgatta
      361 gttgccatag gatggccctc ctttttgttc tttatgtaca cgttttctca tagtcgaact
      421 ggctgctttg agtccggcaa gtaaactgga aacgcccaca gcaagggcac cccctttccc
      481 gcgaacggtc tgatgaacat gatttacggt ttcccccaca tctccaactg cattcaagaa
      541 aatggtggcc cgatccatgc caagcctggc ttgatcggtt aacgctaaaa tgttttcgtt
      601 ggtccccttg acgtccttca ataatcccgg caattcatgg ttcaggcgaa tcagcaagcg
      661 ttctgattgc ccaacggtgc gtttcacttg aagaatcatt ggaaccagat agcccaccaa
      721 gaccacaaag gccacggcaa tgatgatcgc tgccaaatcc accatacctc gctatccttt
      781 catgaccgaa tcacgggtcg tttcattcgc cagtccgtgg cttgttcata ggcataggct
      841 gcacgcaaga ccatggcttc ttcaaacgga cgccccatca gttgtaatcc gataggaagt
      901 cccattttac tcaaaccaca gggaaccgaa atcgcgggaa gtcctgacaa actcgcagga
      961 atcgtataaa tgtcggacaa atacatctgc aagggatcgt ccaagcgctc tcctaattga
     1021 aaggcgggcg tcggcataac cggactcacc aacagatcga cttcctggaa cacggcatca
     1081 aattcctgtt gaatcaaggt gcgaacggct tgggctttcc cataatacgc ctcgtaatac
     1141 cccgcactca gggcatacgt tcccaacatg atacgccgtt tcacttcagg gccaaaccct
     1201 tgcgaccggg tgctccgata catctctgcc aattccctgg actccttagc acgaaaacca
     1261 aacctgaccc catcatagcg ggctaaatta gagctggcct ctgccgtggc aatgagataa
     1321 taggtcgcaa tggcccgttc agtggaaggc aacgtcactt ctcgaatggt gccgcctagc
     1381 tcctccaaca cctggatggc atcttgaacc ttttcgtaga cttccgtatc aagcccctca
     1441 gaaaaatatt cccgcggcac cccgataatg agttttttga tgtctttctt cttcacggct
     1501 ttggtgaaat ccgggcagtc acgattagca gaagtggaat ccatcgggtc aaatccggca
     1561 atggcattca gcaggatagc ggcatcagtt acggttttgg tgatagggcc gatttgatcc
     1621 aaggatgagg caaaggcaac taatccaaag cgagacactc gcccataggt tggctttaac
     1681 ccaactaccc cgcaacaagc cgcaggttgt cggattgacc ctccggtatc ggagcctaat
     1741 gccgccacac attcatcggc tgccaccgca gccgccgatc ctccactgga tcctcccggt
     1801 ataaactttt gattccacgg atttctggac ggcccgaacg ctgaatgttc cgtggacgaa
     1861 cccatggcaa attcatccag attggttttg cccagaatca agggttggtg atttcgaaga
     1921 cggcccatca ctgtggcatc ataaggagga acaaaatttt ccaacattcg agatccacaa
     1981 gtcgtccgga tcccttctgt acaaatatta tctttaatgc ctaatggcat gcccataagc
     2041 ggctgtgttt ttcgccaggc tttcagccca tcatcaatcg ctttggcttg ttgaagcaga
     2101 gtctcttttg gtgtcaaggt aatataggct ttgattttcg gctccacctg gctgagccgc
     2161 agcgaataag acctggcgat atccacagca ctaatctcgc cagcggtaaa tttaccctga
     2221 agctctttta gcgtaagttt aaatagagac atacaaaccc gattctggga ttagtgggta
     2281 aaaaggaaat ggaaagaaac aggccttcaa ccggagcaac ccggtcacac gggaattggt
     2341 tactttgctt tattctggtg atcgacttgg ataatttgag ggacgtgccg cttggcttct
     2401 tcgtaattaa ataattcata acaaaaaata atgaccttat cgccaatcac tcccaacctg
     2461 gctgtgggac catttaaaat gatctctccc ttgcctcggg tacctggaag cgcgtaagtc
     2521 gaaaaccgct ctccgttatt caaattggat accatcaccg cttcatagga cataataccc
     2581 gccctctcca tcaggtcttc gtcaatcgtt aaactaccct catactccag actggaaccg
     2641 gtgacggtcg ctcggtgaat tttacatcgt aacaattgtc gatacatatt aaattcctat
     2701 tattttactt gtcatgctca aaccgagcgt tctcaatatc tcgcgcgggc tctattttcc
     2761 taaaatattc ggaacgacga aaaatccatt atgtgactca ggggcattgg ccagcgcctt
     2821 ctccacagac aagcattcgc gtgggctatc ctcacggaat acctgttcct catgcgcgac
     2881 ggaggccgtg ggagggacgc cctccgtcga caccccttgc aattgatcca catacgataa
     2941 aatatgattc aattggtcgg caaacatggg tttttccgct tccgataagt ctaagcgggc
     3001 taattgggcg acatgttcga cttctttttg agtaatggag gccatatctg atccttatga
     3061 tgaagcagga atttaaaaaa catggagaca ctgcaagcgg aaaccacggc accacgtatt
     3121 tttaccatga atttctcatg aaaagctact cagaacaaag gattgaagca ggggtgggaa
     3181 aacgtggaga tcaagaaaaa aagctcgaga aagaacctga ggcaatcaat ggtccaacgt
     3241 gggcgttgga gaaaaaagaa agccttcgtc cgcaaaactc acatagcgcc catccccaat
     3301 aacaatatga tccaataccc ggatacccaa aatttcccct gctgccataa gtcgacgggt
     3361 taaggcatga tcctcctcgc ttggatgagg gtcaccactt ggatgattat ggacaaagat
     3421 gactgctgcc gcagattctc gtacggccag attgaacact tcccgcggat gaacaatact
     3481 caccgtcaaa cttccctcgg agacggtgac atctcgaata agcccatgct tggcatctaa
     3541 caacagggct ttaaagacct catgccgaag atctcgcaac agtggatagt aatgctggta
     3601 aatatcatgg ctatggtgaa tccgtaatcc cgccgttaac ggaaccgcca atgcccgttt
     3661 tcccaactca acggctgcga gaatctgggc agcctttgca ggaccaattc ctggcacaac
     3721 acacagttct tgcaagcttc gattggccaa cccccgcagt cccttgagcc gggtgaggag
     3781 gtccaatgct acatccactg ccgaggaatc gggtcgaccg attcgtagga ggatggccac
     3841 cagttgagca tctgataaac cctgagcgcc attgttcaga aggagttcgc ggggccgttc
     3901 cttctcaggc caggattgaa tgcctttttt gagttgtgga tcattcatac tcataattcc
     3961 agggtatgcc atgcgagcgt gcgtgaacta tacgcagcta tttttcaccc cgtcaaaaac
     4021 gattcttccc attctgtcat tgagattgtt gaggtttccg gaattatgat atcgtccact
     4081 cccatatgaa accatcttct atccggatcg ttgcagggca atttaaaggt cgaacaatcc
     4141 cgacacttcc cgggcgaacc acccgtccga cctctcagcg ggccagagaa gcactgtttt
     4201 ctattatggg tggccagatt caggatgcca ttgtggctga tctattcgcg gggacaggag
     4261 cagtgggatt ggaagcctta agccggggag cgactaaggc actctttgtt gagcaaaatc
     4321 cattggccgg aacgactatt cagcagatgc ttacccaact caaggttacc ccatcaagcc
     4381 aggtcctcat tgaggatgta tcactcgcca tacaaaattc aattttggta ggatggcgtc
     4441 cgtttgacat gatattcatg gaccctcctt accggctccc gaatgttcaa cagatcttgc
     4501 atcaattaga ggaagcggac gtcatggccc cgaccggtcg aatcatttat gaacatttcc
     4561 ataaaaccac tcctccaatt cctcttggga aatggtcgct catccgaacc gcccgctatg
     4621 gggatacggc gttgacgttt taccgacccg ttcccccgac cgtaaaagag cttgaccaat
     4681 gactattggc atttatccag gtacatttga tcctattacc cggggccata ccgatgtcat
     4741 tgctcgaagt ctcaggctgg tcgagcaggt gatcgtcgcc gtggctccca accctcgaaa
     4801 agcccctttg ttcgacttag acgaacgagt ccaattggcc aaaatcgcca caagcgacct
     4861 caagggtgtc acggttgaac cattcgacgg cttactggtg aactacgtca ggaaacgcgg
     4921 agctttggct attattcgtg gactaagagc cgtgtcagat ttcgaacatg aatttcaaat
     4981 ggccctgctg aaccgaaaac ttgactctag tctggaaacg gtctttctga tgtcgagtga
     5041 ggaattttcg tatctcacct ccagcatggt caaagaggtc gcctcggttg gcggtcccct
     5101 ccaccatttc cttcatcccg aggttgccga atggctccag ggtcgattac ggagaaatgc
     5161 caaatgaacc ttgccacgcg aacgacccgc attaccccct cccccacatt gcaactctcg
     5221 gcaacggtga aagccctggt cgcgcaaggg cagcagatct tcgattttac tgcaggagaa
     5281 ccctccctgg attctcctga agaggccaaa gaggccgcct atcaagccat tcgctccggc
     5341 ttcaccaagt atacggccgt caccggcatt gatgatctaa aagaggccat cattgaaaaa
     5401 tttcagcgtg atcagggact gacctactca aggg
//
LOCUS       DATIAL010000564         3481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1174247, whole genome shotgun sequence.
ACCESSION   DATIAL010000564 DATIAL010000000
VERSION     DATIAL010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174247"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VLA60_17245"
     CDS             <1..434
                     /locus_tag="VLA60_17245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSF11162.1"
                     /translation="FHHVGARPLWDGPFLEPVNGKVTGVFGSRRVINGQNRKPHSGED
                     IAAPKGTPVQAINKGIVVKTVDHFFSGKGVVLDHGVGLFSMYFHLSEVDVTSGQTIQK
                     GEALGKVGATGRATGPHLHWGVHLNGSRINPYALTALPISN"
     gene            552..1109
                     /locus_tag="VLA60_17250"
     CDS             552..1109
                     /locus_tag="VLA60_17250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HSF11163.1"
                     /translation="MRYICVFCGSSLGRDPEFTNMARRLGHALVQQGMGLVYGGGNIG
                     LMGVLASTVLQEGGTVIGVIPKHLAEKELLHQELTTTHIVNTMHERKTLMADLSAGFI
                     TLPGGFGTLEECCEMVTWAQLKLHHKPCGLLNCFGFFDSLLRFFDHQVHQGFLTRTNR
                     ALLLEATTPENLLPLILDRLEPDVR"
     gene            complement(1121..1936)
                     /locus_tag="VLA60_17255"
     CDS             complement(1121..1936)
                     /locus_tag="VLA60_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11164.1"
                     /translation="MLEKHFLSSRLSGRRVRGTQSLFGYGGLVLVMAMSCLAMGASVG
                     FSEDQPQELPKPFHKTTPPLFQEWNFDQSSSSSLPKGFTQAFGGSSGKGEWSVKEDST
                     APSPPHLVEQRLQCGEGWCYQLLIAEDAVVEYVDLSVRLKLNLGTPTGKAGLAYGIQD
                     DKNFFATVVEPRTNEVVAYVVKDGEPTELGRESLILRSGDWHSLRIQRNTIISKEFTE
                     ISFDNHLILDVYDQSFKKGKLGLVVMGDGGFSFDNLRAMELMTNRPLSRPSAY"
     gene            2342..>3481
                     /locus_tag="VLA60_17260"
     CDS             2342..>3481
                     /locus_tag="VLA60_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HSF11165.1"
                     /translation="MTKPAFLAVIFLTGLVWPNISESRSPSPIEMEEWSFQELLPSDS
                     VITLTLQEAVMEALENNLDIQVSRHTRDVRLTDIVFQQSQFDPTVELGGRYDRTVVPL
                     NRPIFGLEGVSLGSIPDTFDQNETNFSLGLKQKLLTGGSYDLTFDTKRNSVAGSTSFL
                     FNPAYSSNLLFNLTQPLLRNFGPTINNIQITLAKNAADVEQLTLLNKILSVIAQVEEA
                     YWELVFARENLKVARATLQAAEELLASNRAKVKAGVMADVEALQAQAGVANQIEQILL
                     AQKTVLDQEDQLRLLLSKSEFNLTQNTPLVPLDPPIQHLQEAPLKENLEVAFEHRPDI
                     LQAKKNIETSNVNTRFAKNQLLPDLSFQGALGLSGLGKNPRDDWDR"
BASE COUNT          931 a          961 c          766 g          823 t
ORIGIN      
        1 cctttcacca cgttggcgcg cgtcctttat gggacggtcc gtttcttgag cctgtcaatg
       61 ggaaagtcac tggagtgttt ggcagtcgtc gagtgattaa tggtcagaac cgaaaacccc
      121 actcaggaga agatatcgcg gcgccaaaag gtactccggt tcaagccatt aacaagggta
      181 tcgtcgtgaa aacagtcgat cacttcttct ccggaaaggg tgtggtcctg gatcatgggg
      241 taggcctatt ttccatgtac tttcacctct cagaggtcga tgtcacttct ggccaaacaa
      301 ttcagaaagg cgaagcatta ggcaaagtcg gagccaccgg aagagccact ggcccccatc
      361 tccattgggg cgtacactta aatggctccc gaatcaatcc atacgcattg accgctctcc
      421 ccatatcaaa ttaattaaag gactccggca ttttgggtat tcctcccctt ctcctgactc
      481 aacgtgaacc gtctcttctt cttatagaca tcagattcat ttcgattacc ggaaaataaa
      541 caccccgtcc catgcgatat atttgtgttt tctgcggctc cagtctcggg cgcgacccag
      601 aatttaccaa catggctcgg cgattgggtc atgcgcttgt ccaacaagga atgggcttgg
      661 tctatggtgg gggaaatatt ggactgatgg gcgtgctggc atcaaccgta ctgcaggaag
      721 gcgggaccgt cattggagtt attcccaagc atctggcaga gaaggaactc ctccatcagg
      781 aactcaccac tacgcatatt gtgaacacca tgcatgaacg aaaaaccctc atggcagatc
      841 tgtcagccgg attcatcaca ttacccggtg gatttggaac attggaagaa tgttgcgaaa
      901 tggtaacgtg ggcgcagtta aaacttcacc acaagccctg tggcctgctg aattgctttg
      961 ggttttttga tagcctgttg agatttttcg accatcaagt ccatcaaggg ttcctcaccc
     1021 ggacaaaccg ggcgttactc cttgaagcca ccactcctga aaacctgctg ccccttatac
     1081 tagaccgact cgagcctgac gttcgctaat agcatccggc ctaataagcc gaaggcctgg
     1141 aaaggggacg attggtcatc aattccatcg cacgaagatt atcaaacgaa aacccgccat
     1201 ctcccattac aaccaatccc aatttgccct ttttaaatga ttgatcgtag acatcaagga
     1261 tcaggtggtt atcaaaagaa atttcggtaa attccttact gataatggta tttctttgaa
     1321 tccggagaga gtgccaatcc ccagatcgaa ggatcaagga ctccctccct aattcggttg
     1381 gttccccatc cttgacgaca taagccacaa cttcgtttgt tctaggctca acaacggtgg
     1441 caaagaaatt tttatcatcc tggattccat atgccaaccc ggctttcccg gtcggtgttc
     1501 ccaaattaag ctttaaccgg actgaaagat ccacatattc aaccacagcg tcttctgcga
     1561 tcagtaactg atagcaccaa ccttcaccgc attgtaatcg ctgctctacc aaatggggcg
     1621 gacttggagc ggtgctatct tctttgactg accattcccc ttttcctgaa gatccaccaa
     1681 aagcctgcgt aaagcccttt ggcaagctac tagatgacga ttgatcaaaa ttccattcct
     1741 gaaaaagagg gggagttgtt ttatgaaacg gcttgggaag ctcctgaggt tgatcttccg
     1801 aaaatcccac ggaagccccc atcgcaaggc aactcatggc catcaccaaa accagtcctc
     1861 catagccaaa taaactttgt gtgcccctaa cccttcgccc agacagtcgg ctagacagaa
     1921 aatgcttttc aagcaatctc gactcctcgt gtttcataat tatccctacc ccaaaaagac
     1981 atgaatatta agtagacgga aaacccttag cgcttaggat gatacgcatt ctcaccggaa
     2041 gtccacaacc cctttttggc agtcaaggcc agccaccccc gtggcaccat gacccgcccg
     2101 ccaacctcac gaagcacgca gatcctgaca ggaaaaactc atgagcccct tcacgcattc
     2161 cactggccct cggatgagat ccaggtggcc ctggacaaca caaacaccat cctttctgtc
     2221 taaccatttt acaggaaaaa ttgcggaaaa aatcgagctt tgatagtttg gatccgacgt
     2281 tggtctcctg gaaatctgaa tcaattaacc agatatgcgc tggaccacga tatttcaagc
     2341 tgtgactaaa ccggcttttt tggccgtgat tttcctcact ggactcgtgt ggccgaacat
     2401 tagtgagagt cgttccccca gccccattga gatggaagaa tggtcatttc aggaactcct
     2461 gccatctgat tccgtcatca ccctgacgtt gcaggaagcc gtcatggaag cattagaaaa
     2521 taatctggac attcaagtga gccgacacac gagagacgtt cgactcaccg acattgtctt
     2581 tcaacagtca caatttgacc ccacggttga gctcggcgga cggtacgacc ggacagtcgt
     2641 tccactcaat aggcccatat tcggccttga aggcgtctcg ctgggttcga ttccagacac
     2701 ctttgaccaa aatgaaacaa acttcagcct gggattgaag caaaagttgc tgaccggcgg
     2761 atcgtacgat ctgacctttg atacaaaacg gaattccgta gccggatcaa ccagctttct
     2821 ttttaacccg gcctattcaa gcaatctact ctttaatctg acgcaaccac ttcttcgcaa
     2881 cttcggaccc accatcaaca acatacaaat taccctcgct aaaaacgcag ctgacgtgga
     2941 acaactgaca ctcctaaata agatcctgtc ggtgatcgcc caggtcgagg aggcgtattg
     3001 ggaactcgtc tttgcccggg agaacttaaa agtcgctcgc gcgaccttac aggccgcaga
     3061 agaattgctc gccagcaatc gcgcaaaggt taaggccggg gtgatggccg atgtagaggc
     3121 ccttcaagct caggcagggg tagccaacca gatcgaacaa attttgctgg ctcaaaaaac
     3181 cgtcctggat caggaagacc aacttcggct cctccttagt aaatcggaat tcaacctgac
     3241 ccaaaacacg cctctcgtcc cactcgaccc acccattcaa catcttcagg aagccccgct
     3301 caaagaaaac ctggaggtag cattcgagca tcgtccggac attcttcaag ccaaaaagaa
     3361 cattgaaacg tccaacgtca atacccgctt tgccaaaaat cagcttctac ccgacctctc
     3421 gtttcaggga gccctgggtc ttagcggact ggggaaaaac ccccgggatg actgggaccg
     3481 t
//
LOCUS       DATIAL010000565         8865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_100189, whole genome shotgun sequence.
ACCESSION   DATIAL010000565 DATIAL010000000
VERSION     DATIAL010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100189"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..464
                     /locus_tag="VLA60_17265"
     CDS             228..464
                     /locus_tag="VLA60_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11166.1"
                     /translation="MDIFGKIPLVEIPVHVTPDQKKWLDKMVEEGKIAIPPGGTLEKG
                     SVISMFLRMLIHNAMEEQMRQAAIDAEDEEDDDE"
     gene            complement(565..1635)
                     /locus_tag="VLA60_17270"
     CDS             complement(565..1635)
                     /locus_tag="VLA60_17270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF11167.1"
                     /translation="MAKRYFLESDNGGFIGSIVKHWINGERPVPPFPKTVQIQTLSGC
                     NARCQFCPNGHTTNRLSMGSMSEDLFRKIVDEVVQHPVRRISPYLMNEPLADPRLPDL
                     IRYITERKRKETSTSISTNATYLTSEMGERLIESGLDSLMISFHGIRKETYETSMGNL
                     DWEIHLNRVNEFIELQRRRKSKKPVVAVTMVHTATLDQELGEIRDYWNSRGVVVRIHT
                     LENRSHPWVDGKAINVKPMKPYTNCDRLMGQAYLLWNGDVVLCCVDWERTTIFGNVTN
                     QSVESVWQGETYTGYRRNYLAGKIGGTLCDGCKVQDEGDFKFKPSWSIKGLIKGAPYH
                     YKPLPPPLPGKTNKKSLSVRSI"
     gene            complement(2158..3666)
                     /locus_tag="VLA60_17275"
     CDS             complement(2158..3666)
                     /locus_tag="VLA60_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HSF11168.1"
                     /translation="MSMATSTERLHPAIQSFVSHPRKMLINGHWVNAQSGKTFSTYNP
                     ATGEVMAEVAEGDKADIALAVKAARKAFEQGPWRKMTPSDRGKLLWRLADLLESNLEE
                     FAQLESLDNGKPLTIARVADVPLAVDLFRYMAGWSTKIEGNSIPISVPYMPGSQFLAY
                     TLREPVGVVGQIIPWNFPLLMAAWKLGPALAVGCTIVMKPAEQTPLSALRLGELFIEA
                     GFPEGVVNIVPGYGETAGAALAAHPDVDKIAFTGSTEVGKLIVNAATGNLKKVTLELG
                     GKSPSVVLPDADLETGIPGVASAIFFNHGQCCAAGSRLYVEKGIFDKVVAGVADQAKQ
                     IKIGPGLDPTTQMGPLVSDEQQHRVLGYLESGFAEGAQAVAGGKRSGDKGYFVEPTVL
                     VNTNSTMKVIQEEIFGPVVCAEPFTEVEEVIAKANDNIYGLAAAVWTKDLSKGHRIAA
                     QLRAGTVWINCHNIFDASLPFGGYKQSGWGREMGHEALDLYTEVKSVCAKLA"
     gene            complement(4367..5653)
                     /gene="hemL"
                     /locus_tag="VLA60_17280"
     CDS             complement(4367..5653)
                     /gene="hemL"
                     /locus_tag="VLA60_17280"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HSF11169.1"
                     /translation="MKTTRSRALFQRANAFIPGGVNSPVRAFRSVGGDPLFIKQAKGI
                     LLEDVDGNKFLDYVLSWGPMILGHANSRVLKAIISASKRGTSYGAPSEGEVELAKIII
                     KMVPSIEKVRLVNSGTEAVMSAIRLARAYTKRDSILKFEGCYHGHADHLLVKAGSGVA
                     TLGIPDSPGVPESFARHTLTAPYNDMKTTEKLIHKHAQNLGAIIVEPIAGNMGVIPPS
                     DEFLPLLRMITKKYDILLIFDEVISGFRVDPGGAQALYKVIPDLTILGKIIGGGLPIG
                     AYGGTKDIMQMIAPAGPVYQAGTLSGNPVAVAAGLETLRILQTPGVYKELEKRSKVLA
                     DGLGEAAKEAGIPYYQTRVGSMLGGFFTEGPVTDYAEAKKSDTVRYAKFFHGMLNHGI
                     YLAPSQFEAMLLSTAHTNRHIHQTIAAAQEVFSTLR"
     gene            6111..6464
                     /locus_tag="VLA60_17285"
     CDS             6111..6464
                     /locus_tag="VLA60_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HSF11170.1"
                     /translation="MYCEQRRKNDITILDCFGRFDEGDTNQFIQWLEGLQNHGIQHLI
                     LNLSPLYYLDPKVVNLLFFAQEFFQANSGSFSLVSPLSSVRNELVRGKIPHTIPTFDT
                     MYDAMHRPHCAYNEC"
     gene            complement(6534..6650)
                     /gene="rrf"
                     /locus_tag="VLA60_17290"
     rRNA            complement(6534..6650)
                     /gene="rrf"
                     /locus_tag="VLA60_17290"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(6754..>8865)
                     /locus_tag="VLA60_17295"
     rRNA            complement(6754..>8865)
                     /locus_tag="VLA60_17295"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2260 a         2361 c         1879 g         2365 t
ORIGIN      
        1 atgcctccaa gccattcgat cgggcacctt ctaaccctgg cctaaaagac taccctcacc
       61 ctgtcgattc acgattcttt cattgacaaa gccaaagagc gcgtggtctt atcgaaagac
      121 gcacgaggac cacattcact catcaatacc tcgctttgaa tttcaccgga caaggtaagg
      181 gattagcgaa aatatatcag gttcatgtaa aaaggagact ggctaccatg gatattttcg
      241 gaaagattcc tctcgtggag attcccgtgc atgtaacccc ggatcagaag aaatggctgg
      301 ataaaatggt agaagaagga aaaattgcca ttcccccagg tggaactctg gaaaaaggct
      361 cggttatttc tatgtttctc agaatgctca tccacaatgc catggaagag cagatgcgcc
      421 aagccgccat cgacgccgaa gacgaagaag acgatgatga atagttacat tcaacacttt
      481 tccgtatgct ctagtgcgaa gatttgaatt tggagcccgg aagagcccaa gtcagacgct
      541 tattaggtat aaggagaaaa tcctctatat agatcgaact gacaatgact ttttgttggt
      601 tttcccagga agaggagggg gaaggggttt atagtgataa ggggcacctt ttatcaggcc
      661 ttttatggac cacgatggtt taaatttaaa atccccttcg tcttgcactt tacatccatc
      721 acataatgtt cccccaatct tccccgccag gtaattgcgc cggtaccctg tataggtttc
      781 cccctgccac acactttcca ccgattgatt agtcacattt ccgaaaatgg ttgttctttc
      841 ccaatccacg cagcacaaca cgacatcacc attccaaaga agatacgcct gacccattaa
      901 ccgatcacaa ttggtatacg gcttcattgg cttaacattt atggccttgc catcaaccca
      961 tggatgagac cggttttcca gggtgtgaat tctgacaact accccgcgag aattccaata
     1021 atcccgtatt tcccccaatt cctgatcgag ggttgcggtg tgaaccatgg tcacggccac
     1081 aaccggcttc ttggattttc tccgcctttg gagttcaata aactcattga cccgattcag
     1141 atggatttcc caatcaaggt tccccatact ggtttcatag gtctccttcc gaataccgtg
     1201 aaaactaatc atcagactat caagcccgct ctcaattaaa cgttcgccca tttctgatgt
     1261 caaataagtg gcattggtgc taatactcgt actagtttct ttcctctttc gttccgtgat
     1321 gtagcggatg agatctggaa gccgaggatc cgctaacggt tcattcatca ggtacgggct
     1381 aatccttctc accggatgct ggacgacctc atccacaatc tttctgaaaa gatcctcact
     1441 catgctcccc atcgaaagtc ggtttgtcgt atgaccgttt ggacaaaatt ggcaccgggc
     1501 attacaaccg gacagggtct gtatttgaac ggtcttggga aatggtggga ccggacgttc
     1561 cccattaatc caatgtttaa cgattgaacc gatgaaacca ccattatccg attcaagaaa
     1621 atatcgtttg gccacacggt tacctcacaa tagataattt tggatgtcct tcatgccggc
     1681 atgctaaatg aaattggaca tgtgtggttc atttatgagc ttacacactc ctgtcaaaac
     1741 catctagaaa tttcccccgt ccacacccca aagtagaagt gcccggcagt taagctttca
     1801 tcgccatcag tcacgccttt tcggcagcag aggcttgcgc gccaagcacg agccactatc
     1861 ggcgtaaccg gaaatccctc gtaaaccgca gtccaatcgc ggagcaaccc actgccgtcg
     1921 ctaaacaccg aatgcagtcg agagctcggc agaacgccct cgaagccgag accgacgaca
     1981 aatagggatt gcatttccct ctcgccgaac ccccaatccg atgcttcccc cagcataagt
     2041 tttttgaggc tttcaatgtt agtttatcca ccggcttctt cttcaaccga agaaggaaaa
     2101 ttggggggtt atacgaaatc ttcttttcca aaagttcctt agaatttcgt tccaatctta
     2161 ggccagtttg gcacagacgg atttaacttc ggtatagaga tctaatgctt catggcccat
     2221 ttcgcgtccc caaccggatt gtttgtatcc gccaaacggg agggaggcat caaaaatatt
     2281 atggcaatta atccaaaccg ttccagcccg caattgcgcg gcaatccgat gtcccttact
     2341 caaatctttt gtccagaccg ctgccgccaa tccatagata ttatcattgg ccttggcgat
     2401 cacttcttca acttctgtga aaggttcagc gcacaccaca ggaccaaaaa tttcttcttg
     2461 aataaccttc atggttgagt tggtattgac caacacggtc ggttcaacaa aatacccctt
     2521 gtctccagag cgtttcccac ccgcaaccgc ttgagcgcct tcggcaaacc ccgattccaa
     2581 atatcccaga acacggtgtt gctgttcatc agagactaat ggccccattt gagtagtagg
     2641 gtctagtccc ggtcctatct taatttgttt ggcttgatcg gcaacccctg ccacgacctt
     2701 gtcaaaaatg cccttttcca catacagccg tgagcctgcg gcgcaacatt gcccatgatt
     2761 aaagaaaatc gcactcgcta ctccgggtat tcctgtttcc agatctgcat ccggcaggac
     2821 aacacttggt gatttgccac caagttccaa tgtgactttt ttaagatttc ctgtcgcagc
     2881 attcacaata agtttgccaa cttcggtcga tccagtaaat gcaattttat ccacatcagg
     2941 gtgcgcagct aaggccgcac ctgcggtttc cccatagccg ggaacaatgt tcacgactcc
     3001 ttcgggaaat cctgcctcga tgaacaactc tcccaaccgc aatgccgaca agggagtttg
     3061 ttcagccggt ttcatcacta tagtacatcc cacggccaaa gccgggccaa gtttccatgc
     3121 cgccattaac agaggaaaat tccagggaat aatttgcccg acaactccaa ccggctcacg
     3181 aagggtatag gccaggaatt gagacccagg catataggga accgaaatgg gaatggaatt
     3241 gccctcaatt tttgtggacc atcccgccat atatctaaac aaatcaacag ccaatggaac
     3301 gtctgctacc cgcgcaatgg ttaacggctt accgttatcc agagactcca actgagcaaa
     3361 ctcttccaga ttcgactcca gaaggtccgc caaccgccac aggagtttgc cgcggtcaga
     3421 aggcgtcatc tttcgccatg ggccttgttc aaaggccttt ctggcagctt tcacggcaag
     3481 agcaatatca gccttgtcgc cttccgccac ctccgccatt acttcacccg ttgcaggatt
     3541 atacgtggaa aatgttttgc ctgattgtgc gttaacccag tgaccattaa tcagcatttt
     3601 tcgtggatgg gacacaaaag attggatagc cggatgaagc cgttcggttg aagtcgccat
     3661 actcatttgg gtacctcctt tttttccaga aaaatcacct gggtcgggtt tcaagaaaca
     3721 ggcctatgaa ttattgcctc aaaaacatac gaggttgcaa atccgatatt gtcgctggct
     3781 ccctccttgt tttcgacttc acctatgact ccaaggatat ggaattacgt cttattatgg
     3841 cgacgattca atttctaaag caaaccttag tccgttaggt tgtccttcgg tgtcctagaa
     3901 caaaccattt ttgagcagtc tttaaattgg tcgcgatacg atgccttgcc tattatgcga
     3961 acttttccac aaaattccat tttttccaat agatgggtat agacagccag tggggttaca
     4021 gaatacctgc taattgcttc cagagcttgc catcacggac tgttgaatat tttttaaata
     4081 agtgacagaa ctcccccatg aggaaaaata atggagtgct caaaatgact catcccgcaa
     4141 gcccgcagcg gccttggcaa acgtagggta cggcggaata cgtgagcacg accaagagcg
     4201 agaatgacgc cggcaggatt tttcaccatt cctaatcagg acaatgaatg gaaggcatcc
     4261 ttcggccatt cgtagaaaaa aagctgatgg gtaaaccatg ggagattggc tggcaatcaa
     4321 taaaccaaac gccaaaccaa attattgctt tgcaacaaaa aggagattat cttagtgtag
     4381 aaaagacctc ttgtgcggca gcaatcgtct gatgaatgtg tctattggtg tgagccgttg
     4441 agagcaacat ggcttcaaat tgagagggag ccaaatatat tccatgattg agcattccat
     4501 gaaaaaattt tgcgtatcgt acggtgtctg actttttggc ttcggcgtaa tcagtgacag
     4561 gtccttcggt aaaaaatcca cccaacatgg agccaactcg agtttgatag tagggaatcc
     4621 ctgcttcctt agctgcttct cctaatccat cggccaacac tttggatctt ttttctaatt
     4681 ctttatagac gccaggtgtt tgaaggattc ggagagtctc tagtccagca gccactgcca
     4741 ctggatttcc ggagagagtt ccggcctggt acacagggcc ggcgggtgca atcatttgca
     4801 taatgtcctt ggtgcctcca tatgctccaa tgggtaagcc tcccccgatg atcttcccta
     4861 aaatcgtgag gtcaggaatg actttataga gggcttgcgc tccacccgga tccactctaa
     4921 aacctgaaat gacttcatcg aaaattaaga gaatatcgta ctttttcgtg atcattcgga
     4981 gcaatggcaa aaattcatcc gacggaggaa tcacccccat atttcctgca ataggctcta
     5041 caataattgc gcccaaattt tgggcatgtt tatgaatgag tttttcggta gttttcatat
     5101 cgttatatgg tgccgtcaat gtatggcggg caaacgattc tggtacccca gggctatcag
     5161 gtatccctag ggtagccacg cctgatccgg ctttaacgag caaatggtcg gcatgtccat
     5221 gatagcagcc ctcgaatttc aatattgagt cacgtttagt gtaagcacgg gccaaacgaa
     5281 ttgcgctcat aacggcttcc gtccctgaat ttacgagacg gactttttca attgagggaa
     5341 ccatcttgat aataattttg gctaattcca cttctccttc ggagggggca ccatagctcg
     5401 tcccgcgttt tgacgcactg atgatagcct ttaatacccg cgaattagca tgcccaagaa
     5461 tcataggccc ccaagataaa acataatcca aaaatttatt gccatccacg tcctctaaca
     5521 aaattccttt tgcctgttta atgaataaag gatctccccc gactgagcga aaggctctga
     5581 caggactatt aacacccccc ggaataaatg cattggctcg ttgaaataat gctcgggacc
     5641 ttgttgtttt cacagaatta ttcctctatt tttttttaaa tctatttaat atttcacttc
     5701 caaagaattc acaaatttaa tcttgccatt atactaaata ttcataactc accgaaatta
     5761 ttgcgaaacg tttaattaat tccccccagt ctgactccaa tttcacagag cttcaaacgc
     5821 gccactactg ttttactaga gaaacatcca gccatccaat ttcccccaac ttttaccatc
     5881 caaaccttgg gaatttttca agacggttac catttaaaat atttccatgt agctccaaat
     5941 tcttctcgca aacaattatc gcaaaaattt tcgaaatatt ccggccttct ttaaatacgt
     6001 gtagtgcgtt cagtgcaaaa ttttcctatt tattttttca acttttaacc gaaaattcta
     6061 gaataccgtc aacaataaat aatttttttt aatcatattg ggataaacca atgtattgtg
     6121 aacaacggag aaagaatgac ataacaattc ttgattgttt tggaagattt gatgaaggtg
     6181 ataccaacca atttattcag tggcttgaag gattacaaaa tcatggcatt caacatttaa
     6241 tattaaattt gtctcctttg tactatttgg atcctaaagt ggtgaatctc cttttttttg
     6301 cacaggaatt ttttcaagcg aattcagggt cattttcatt ggtaagccca cttagttcgg
     6361 ttaggaatga acttgttcga gggaaaattc ctcataccat accgaccttt gataccatgt
     6421 atgatgctat gcatcgccca cattgtgctt ataatgaatg ttaaacttaa tatggacttc
     6481 tctgaaatag aaaagcccag ggatttatca tccctgggct tttcctattt aaatctcggc
     6541 aacgacctac tttcccacag cctcacggct gcagtatcat cggccctgga gggcttaact
     6601 tccgtgttcg aaatgggaac gggtggtacc cctccggtaa ggtcaccgag aaagtgtctg
     6661 cgaatagtaa atattaattt aaaaaagttt taatatgcta atttttaaat caaactcaca
     6721 tattcactta gaccatgtct atcaagaaca aatgttgtta agtcgcacgg ccgattagta
     6781 ctggttagct tcaggcctca cagcccttcc acacccagcc tatcaaactc gtagtctaca
     6841 agtggccttt agggaggtta ccctccgtgt gatctaatct tgaggctagc ttcccgctta
     6901 gatgctttca gcggttatct aaaccggaca tagctacccg gcactgccgc tggcgcgaca
     6961 accggcacac tagcggtccg tccatctcag tcctctcgta ctagagacag gccctctcaa
     7021 atcacctccg cccacaacag atagggaccg aactgtctca cgacgttctg aacccaactc
     7081 tcgtaccgct ttaacgggcg aacagcccga cccttgggac cggcttcagc cccaggatgc
     7141 gatgagtcga catcgaggtg ccaaacttcc ccgtcgatgt gaactcttgg gggaaatcag
     7201 cctgttatcc ccggcgtacc ttttatccgt tgagcgatgg cccttccacg cagaaccacc
     7261 ggatcactaa gcccgacttt cgtccctgtt tgagctgtcg ctcttacagt caagctccct
     7321 tttgccttta cactcaatgg ctgattgccg accagcctga gggaaccttt gggcgcctcc
     7381 gttacctttt gggaggcgac cgccccagtc aaactaccca ccagacaatg tcctcaccct
     7441 ggatgacagg gccaagttag aatgtcagaa aagcaagggt ggtatttcaa ggttggctcc
     7501 acgaaagcta gcgctcccgc ttcatagcct cccacctatc ctacacatac tcgcccaaca
     7561 cccaatgtca agttgtagtg aaggtgcaca gggtctttcc gtctagttgc gggcacccgg
     7621 cttcttcacc ggaacaacaa gttcgctgag tctctccccg agacagcgct ccagtcgtta
     7681 tgccattcat gcaggtcgga acttacccga caaggaattt cgctacctta ggaccgttat
     7741 agttacggcc gccgtttact ggggcttcca ttcggagctt cgccttgcgg ctgacccctc
     7801 cagttaacct tccagcaccg ggcaggcatc agaccctata cttcctcttg cgagttagca
     7861 gagtcctgtg tttttggtaa acagtcgcta gagccacttt actgcgacct cgctcggctc
     7921 ggcctgtacg gcctcaccta cacgaggcac cccttcttcc gaagttacgg ggctagatgg
     7981 cagagttcct tagggagtgt tctctcacgc cccttggtgt attccacccg cctacctgtg
     8041 tcggtttgcg gtacggtcac cacgcgaact cgctacgagg cttttctcgg cagtgtggga
     8101 tcagtccgtt tgtggccttg cggcctcccc atcacacctc ggcggtaacg gacccgcgga
     8161 tttgcctgcg agccccgcct acatgcttgg accgggtatt ccagggaccc ggcggtacct
     8221 accctcctgc gtcccccctt cgctgataac gtccacgcgg tggtacagga atgttgacct
     8281 gttttccatt gcctacgcct ttcggcctcg gcttagggac cgactaaccc tgacctgacg
     8341 aacataggcc aggaaacctt aggcttacgg ggacgatgat tctcacatcg tttatcgcta
     8401 cttatgccgg cataatctct tctctgcgct ccagcagtcc ttgtcggtct accttcgccg
     8461 cacagagaat gctcccctac cactcgcccc ttgcgggaca agtccgcagc ttcggttgtg
     8521 aacttcagcc ccgttacatt ttcggcgcac gatcgctcga ccagtgagct gttacgcact
     8581 ctttaaagga tggctgcttc taagccaacc tcctggttgt ctgggcgacc ccacatcctt
     8641 tcccacttag cacacactta ggggccttag ccggcgttct gggctgtttc cctcttgacg
     8701 acggagctta tcccccgccg tctgactccc acgctcatag atgcggcatt cgcagtttga
     8761 ttgggtttgg taatctggta ggacccctag cccattcaga gctctaccac catgcgatct
     8821 acgcgaggct agccctaaag ctatttcggg gagaaccagc tatct
//
LOCUS       DATIAL010000566         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1499299, whole genome shotgun sequence.
ACCESSION   DATIAL010000566 DATIAL010000000
VERSION     DATIAL010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499299"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VLA60_17300"
     CDS             <1..693
                     /locus_tag="VLA60_17300"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HSF11171.1"
                     /translation="VGGGPSGLLCARDLAATGFRTLLIEQSLALGGGFWSGGFLMNKA
                     TICEPADQILEELGIPFKPIRDCPGMTMVDPPHVTSRLISAAYEAGVKIMNLTKVVDL
                     ILRQDHRLEGVVVNNSTVEMAGHDTIHVDPIALESQIVIDATGHDAVVIELLHKRNLY
                     QKIPGNGAMWVARSEALVVENTREIYPNCFVTGLAVAAVDGSPRMGPAFGSMLLSGRR
                     AAALVREKLKGE"
     gene            739..1254
                     /locus_tag="VLA60_17305"
     CDS             739..1254
                     /locus_tag="VLA60_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF11172.1"
                     /translation="MDIQDFLDQGEGREEDKIAAWNLFQRAYELQMKGQLEEAVDLYK
                     QSIDTFPTAEAHTFLGWTYSFMGQIHEAIEECHRAIRQDPEFGNPYNDIGAYLIELNQ
                     LEDAVPWLEKALKAKRYENPAFPHMNLGRVHERKGEWDDAVDSYKKSLALNPEYKTAK
                     QALMRILTLMN"
     gene            1275..1658
                     /locus_tag="VLA60_17310"
     CDS             1275..1658
                     /locus_tag="VLA60_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HSF11173.1"
                     /translation="MSATKTILVAVSDIFFYTKIRDAFLPHGYKLERLRTGNDWQIQA
                     LQCQPMGIIINMNDERLNATETLKALQALPQTQSLPILAFANHEEIGMWKQAKDLGIQ
                     KMVSRNEFSARTRALFEEITASATS"
     gene            1655..2752
                     /locus_tag="VLA60_17315"
     CDS             1655..2752
                     /locus_tag="VLA60_17315"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HSF11174.1"
                     /translation="MKTLPLHTDHQALGATFRPCGAWDIPFHYGNPLLEYEVIHQRAG
                     LADLSLRGKILVTGDDRVTWLQSIISNDILPLKPGQGRYSAFMNHKGKMLSYFRVFLQ
                     SDTLIIEDVGEVGDLTYQNLRKFMLYGTKAKLHNGLDSWGILLVTGPKGPEILKQALG
                     VEVGPLQILDSITFTLGDIQGFLARTEETGGQDYELFVPVEALSSLWSHLLRTGKAGG
                     LQPVGREALDTSRIEGGLARLGPDLNEKIVPPEANLEGIAFSLSKGCYPGQEVVARMD
                     TYGSVKRRMVGLIIETADRQLPEIGAKLFSGTREVGWATSSTYSPLLQKAIAFGFPLR
                     DFTQPHTKLEIEILGHRVPASVSPLPFTAHQ"
     gene            complement(2792..>3264)
                     /locus_tag="VLA60_17320"
     CDS             complement(2792..>3264)
                     /locus_tag="VLA60_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11175.1"
                     /translation="ATDVFEQTRAAALRATGPDERALQIDYPALASKIDRALAGRKIN
                     LLHVNEFLPEGYEDQGRFNVIVMTLSKVLFDMVIGDSYFRYDIFSCGDLDKIQLIDGT
                     WDNKEKRTEESFLSLRLMHGDEAHILLALNEDQRPTVLSFAEKISHGRHPEQVA"
BASE COUNT          878 a          812 c          779 g          795 t
ORIGIN      
        1 gtgggaggcg gtccatccgg gcttctctgc gctcgagatc tcgccgcaac cggcttccgg
       61 actctgctca tcgaacaatc attggcactg gggggcgggt tttggtcggg tggctttctc
      121 atgaataaag ccacaatatg cgaaccagcg gatcaaatcc ttgaagaatt gggtatacct
      181 tttaagccca tcagagactg cccgggaatg accatggttg atccgcctca tgtgacaagc
      241 cggctcattt ccgccgccta tgaagccggc gtgaagatta tgaatctcac aaaagtcgtg
      301 gatttaattc ttcggcagga tcatcgccta gaaggtgtgg tggtgaataa ttccaccgtg
      361 gaaatggccg ggcatgacac gattcacgtg gatcccatcg cactcgaaag tcaaatcgtg
      421 attgatgcca ccggccatga tgcggtcgtc atcgaacttc tccacaaacg gaacttgtac
      481 caaaaaattc caggcaacgg ggccatgtgg gtggcgcgtt cggaagcctt ggtggtagaa
      541 aatacccgtg aaatttatcc aaactgcttc gtcaccggtt tggctgtggc agcggttgat
      601 ggcagtcccc gtatgggacc ggcctttgga tcgatgctac tttccggtcg acgggcggct
      661 gcattggttc gagaaaagct aaaaggcgaa taacctcctt ttcagaaagt ccttaaaaag
      721 aagtacaata cgactgctat ggatattcaa gattttctag atcagggtga aggcagagaa
      781 gaagataaaa ttgcagcctg gaaccttttc caacgggctt atgaacttca aatgaaaggg
      841 caactggaag aagcggtgga tttatacaaa caatccatcg acaccttccc cactgcggaa
      901 gcgcacacct ttttaggatg gacatatagc ttcatggggc aaatacacga agccattgag
      961 gagtgccacc gggccatccg ccaagacccg gaatttggaa atccctacaa tgatatcggc
     1021 gcctatctca ttgaattaaa ccagctggaa gatgccgttc cctggcttga aaaagccctg
     1081 aaagccaaac gatatgaaaa ccccgcattt cctcatatga atctaggccg cgtacacgaa
     1141 cggaaaggcg agtgggacga cgcagtcgat tcctacaaaa aatctcttgc cctcaacccc
     1201 gaatataaga cggcgaaaca agcgcttatg cgtattctga ccctcatgaa ctagaaagat
     1261 gtaaaggctt ttacatgtct gccacgaaaa cgattcttgt agccgtcagt gatatctttt
     1321 tctacaccaa aattcgggat gcctttttac cccatgggta taagcttgaa cgtcttcgca
     1381 cagggaatga ttggcaaata caagctcttc agtgccaacc aatgggcatt attatcaaca
     1441 tgaatgatga acgtctcaat gccaccgaga ctttgaaagc cctccaggca ctgccccaaa
     1501 cacaatcgtt acccattctg gccttcgcca atcatgaaga aattggaatg tggaaacagg
     1561 ccaaagacct tgggattcaa aaaatggtct ctcgcaatga attctctgca cgaacacgtg
     1621 ccctattcga agaaatcact gcatcagcca cttcatgaaa actctcccac ttcatacgga
     1681 tcatcaagcc ctgggagcca cgtttcggcc ttgtggcgcg tgggatattc ccttccatta
     1741 cggcaaccct ctcttggaat atgaggttat tcatcagcga gccggcctcg cagacctgtc
     1801 cttacgaggg aaaattctcg tcaccggcga cgaccgggtc acatggctcc agagcattat
     1861 cagcaatgat atccttcctc tcaaaccagg gcagggccga tactcagcct ttatgaatca
     1921 taaaggaaaa atgctctcct attttcgagt gtttcttcaa tccgataccc tcattattga
     1981 agatgtcgga gaggtgggtg acctgaccta tcagaacctt cgaaaattta tgctctatgg
     2041 gacgaaggct aaactccaca atggcctgga cagttggggg attcttctgg ttactggacc
     2101 gaaagggccg gagattctta agcaggcctt gggtgtggaa gtgggtccgc tccagatatt
     2161 agacagcatc acgttcacgt taggtgatat ccaaggattt cttgcccgca cggaagaaac
     2221 gggggggcaa gactacgaat tgtttgtgcc ggtcgaagct ctttcctctc tttggtctca
     2281 tctcttgaga acaggaaagg cgggaggtct tcaaccagtg ggaagagagg ctctcgacac
     2341 atccaggatt gaagggggac tagctcgcct cggaccggac ttgaatgaaa aaattgttcc
     2401 gcctgaagcg aatctggagg gaatagcttt tagtttatcg aagggatgtt atcccggtca
     2461 ggaagttgtc gcccggatgg atacctacgg atccgtcaaa cgtcgaatgg tggggttgat
     2521 tattgaaaca gcagatcgac aacttccgga aataggagcc aaactgttta gtggaacacg
     2581 tgaagtgggc tgggcgacaa gctccaccta ttcccctctg ctccaaaaag cgattgcctt
     2641 tggatttcca ttacgtgact ttacccagcc tcataccaaa ctggaaatag aaattctggg
     2701 acatcgcgtt cctgcttctg tcagtccctt gccctttact gctcatcaat gagggttgca
     2761 agatacattt cccaacccgt gaaaaaactt cttaggctac ttgttcagga tgacggccgt
     2821 gagaaatttt ctcggcaaaa gacaaaaccg taggacgttg atcctcgttc agagctaaaa
     2881 gtatatgtgc ctcatctcca tgcattaacc gtaaactgag aaacgattct tccgtgcgtt
     2941 tttctttatt gtcccacgtc ccgtcaatca gttgaatttt atccaaatcg ccgcaagaaa
     3001 aaatatcata ccgaaaatat gagtccccga tgaccatatc gaacaggact ttgctcaacg
     3061 tcatgacaat gacattaaaa cgtccttgat cctcataccc ttccggtagg aactcattaa
     3121 cgtgcagaag gttgatttta cggcctgcca aggcccggtc gatcttactt gccaacgccg
     3181 gataatcaat ttgaagagcc cgttcatccg gacccgttgc gcgtaaagcc gccgctcttg
     3241 tttgttcaaa aacatctgta gcag
//
LOCUS       DATIAL010000567         4442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1499979, whole genome shotgun sequence.
ACCESSION   DATIAL010000567 DATIAL010000000
VERSION     DATIAL010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499979"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VLA60_17325"
     CDS             complement(<1..760)
                     /locus_tag="VLA60_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HSF11176.1"
                     /translation="MTLIRFLICILFLLMFPTAPVWSEVDIDMLKKGVVKITAQFPNR
                     QKVGTGFVAGQGKKHVFIVTASHVIEGEAEVPQSIAVTFFSHQEEPLVAEVINKEGGD
                     PRGLALLKVGGDLPDDVQILEWDTQSQFHGGEEVRVIGFPQVGGNPWAVTRGTLSGFD
                     GPILKFSGAVEEGNSGGPLLYRGKVIGVIVEIIRQFGNAKPSQIAQFTVDNWPGFSRQ
                     VRIEPQNISPGSEPDAGDPVGKRLAALVVTTVPKD"
     gene            complement(1186..2655)
                     /locus_tag="VLA60_17330"
     CDS             complement(1186..2655)
                     /locus_tag="VLA60_17330"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HSF11177.1"
                     /translation="MWPLVKIHSVIGLLGFLSWVGMMPWSESVMAQDLDSLKAGVVKI
                     TAINPNRIGTGVIVGVKDRTAYIATASHVIEGDPLPQVTFYQDRSHSYPAKIQGMEGG
                     NPKGLAALVVEGDLPSGIRPLPFHVEGKLTGGEKVTVIGFPRLVPVPWAVAEATITGL
                     VWPDLIFTGPVEEGNSGGPLLSNGKIVGLITEVAGSYKYAKPAVIAQYELKNWELVFS
                     DSSTVITSTVISSAQEQPTVQPKSFGTLPSASSPENQTGLDGPPMVLIQPGSFKMGDW
                     PGRGDEDERPVHEVRLPRTFAMGQYEVTFAEYDHFARATGRILPDDEGWGRGHRPVIN
                     VSWEDAKAYAQWLSEQTRKGYRLPTEAEWEFAARSGGKEEVWAGTSEERQVRNYAVYQ
                     GDKTAEVGNKQPNGLGLYDMSGNVWEWVEDCWHGSYQGAPTNGAPWLEADGGDCGRRV
                     IRGGSWDDVPVNLRTSFRIGGPTVSRYNFVGFRLAQDIP"
     gene            complement(2857..>4442)
                     /locus_tag="VLA60_17335"
     CDS             complement(2857..>4442)
                     /locus_tag="VLA60_17335"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSF11178.1"
                     /translation="THFQRDSFLPSANREPVKGGSPSAMKDVFISYASEDRPVAQQLA
                     ASLEQSGLSVWWDRQIQVGSEWDKTIEDALTSAKCVVVLWTAHAKGSRWVRAEAREAL
                     NTEKVVPVMLEANAIPLAFTGIQALCFLGWEGTAGSKEFEILLSVIRAKLEGKPIELP
                     EASSTKPSLLGKLVALVGVKAGVGGVLAILLIGSYFVRVDPDISVHVETTRMEFSVAP
                     GVDDKRLTDSVPFETLTVENIGKLAISPDRLLVADPADYDMESDSYPPKAWFDIPVNG
                     RTVQFDVGSSGMASEITIEPTDHQEAVAGQLDGIVLTDETVVTMQVSNDNAVTLAFRK
                     KEGPQRVVVSRIHAVQFIQIGLQAPPELSIPFSQDQELTYHIIFEKKPGTMELFGQDE
                     ALVLVMNEMDITGKKPISSSVLPIQSIDFSSQDPGTGERKPPVGFAGTVEYVSPEGMP
                     SVPMGTNAFLTLDDLDHFEITSISIDPIGHKLVVDLKGRAGYVKTGTAGNPQDLRPTL
                     FDKIRYSPLFEPVRKLLGF"
BASE COUNT         1072 a         1289 c         1072 g         1009 t
ORIGIN      
        1 catccttggg aacagtggtg acaaccaacg cggccaacct ctttccgaca ggatcaccgg
       61 catccggttc gctccccggt gaaatatttt ggggttcaat cctgacttga cggctaaacc
      121 ccggccaatt gtccaccgta aattgagcaa tttgagaagg cttggcatta ccaaactgcc
      181 ggatgatttc cacaatgaca ccgatgactt ttccccggta gagcaatggt cctccagagt
      241 tcccttcttc aaccgcacca gaaaatttaa gaatgggacc gtcaaatcca gacagcgtcc
      301 cccgggtcac cgcccaggga ttgcctccaa cctggggaaa cccgatcacc cgcacttcct
      361 cgcccccatg gaattgactc tgggtatccc actctaaaat ctgcacgtca tcgggaagat
      421 ctccgcccac tttcaacagg gccaaccctc tgggatcccc gccttcttta ttgatcacct
      481 cagcgaccaa cggttcttct tgatgagaaa aaaaagtgac ggcgatcgac tgagggactt
      541 cggcctctcc ctcaatcaca tgtgaggcgg tcacaataaa cacatgtttt ttgccctgtc
      601 cggccacaaa gccggttcca accttttgcc tgttggggaa ttgagccgta atcttgacca
      661 cacccttttt cagcatgtcg atatccacct cggaccacac cggtgcagtc ggaaacatta
      721 ggagaaaaag aatgcagatt agaaaacgga tgagagtcat cggtgaatcc tataccgtaa
      781 gcagagggtt caaatgtgct gcaatcttgg cacatctatc ctgagaagcc aagatcatcc
      841 atgaaatcct tattcaaggc aatggtgaaa acttggcagg agaaatggtc tacacgacct
      901 ccaaaacgta tgcagagaga ttccgggcaa tgaccggccc gaatgacggc aaccggtcta
      961 ttttccctca aggctgcttg cggttggcat gcatctcata ttgaaggaca gaaaaaacta
     1021 gaggcgtttc cggccagaga ctcgccggaa tgacaagtag ggtgaccatt agcccacatt
     1081 tttcttttgg cgagcggtgc tagccttcta cctcccctaa cccctcctta caaaggaggg
     1141 gaaatgagaa aaaagaggaa aagggtaaaa ggacagagca aagggttagg ggatgtcctg
     1201 ggcaagacga aagccgacga agttgtaccg ggagacagtg gggcccccga tccggaacga
     1261 tgttcgcaag ttcaccggta cgtcgtccca ggaaccgccc cgaatcacac gccggccgca
     1321 gtcaccccca tcagcttcga gccagggagc accattcgtc ggtgcgcctt ggtagcttcc
     1381 atgccagcaa tcttccaccc actcccagac gttcccgctc atgtcataaa gtccgagtcc
     1441 gttgggttgt ttgtttccca cctcagctgt tttatcgcct tgatagaccg cgtagtttct
     1501 tacctgacgt tcttccgacg ttcccgccca cacctcctcc tttccaccgc tccgcgccgc
     1561 aaattcccat tcggcttcgg tcggcaatcg ataccctttc ctcgtttgtt ccgagagcca
     1621 ttgggcgtaa gccttggcat cctcccacga cacattgatc acgggacgat gcccccggcc
     1681 ccagccttca tcgtcaggta atattcgtcc agtagcccga gcaaagtggt catattcggc
     1741 aaaggttact tcatattgtc ccatggcaaa ggttcgaggg agccgaacct catggaccgg
     1801 acgctcatct tcatcgccac gccccggcca atcacccatc ttgaatgatc caggttggat
     1861 cagcaccatc ggcggcccat ctaggccggt ctggttttcg ggagaagaag cacttggcag
     1921 tgttccaaag gatttgggct gaaccgtggg ttgctcttgg gccgacgaga tgacggtgct
     1981 ggtaatgact gtgctggaat ccgaaaaaac caattcccag tttttgagtt catactgggc
     2041 aatcaccgcc ggtttggcat atttatacga tcccgccact tcggtgatta ggccaacaat
     2101 ctttccattc gagagcaaag gcccccctga attaccttcc tcaaccggcc cggtaaaaat
     2161 caaatcgggc cagactaagc cggtaatggt cgcttcggcc actgcccaag gaaccggcac
     2221 cagtcgagga aatccaatca cggttacctt ttctccgccg gtcaatttcc cctctacgtg
     2281 aaaggggagt ggccgaattc ccgaaggcag atcaccctcc accaccaatg cggccaatcc
     2341 ttttggattt ccgccttcca taccctgaat cttggccgga tatgaatgcg agcggtcttg
     2401 ataaaaagtc acctgcggta agggatcgcc ttcaatcacg tgggaggccg tggcaatgta
     2461 cgccgttctg tccttgactc ctacaatcac gccggtaccg atgcggttgg gattgatggc
     2521 ggtgattttc accactccgg ctttgagcga atccagatct tgggccatga cagattcact
     2581 ccacggcatc atccccaccc aagagagaaa tcccagaaga cctatgacag agtgaatttt
     2641 cacaaggggc cacatctgta tcattcctaa gaatctaaac caagggaaac aggttgttca
     2701 atgagcacag gccacaacag ggcctcccct cttcgtaacg gcgttccggc ggaacgtcgt
     2761 ctttgcccca tgtcaaccac caattcagga tagacgcccc accgtggcgt ctctacagga
     2821 tcacatgcaa acgaggtacg aatcttattc cttgtcctag aaccccaata atttgcggac
     2881 tggttcgaat agagggctgt aacggatttt atcgaagagg gtggggcgaa gatcctgtgg
     2941 attaccggct gtccccgttt tgacatagcc ggccctgccc ttcagatcca ccaccaattt
     3001 atggccgatc ggatctatgc tgattgaggt gatttcaaaa tggtccagat catccagggt
     3061 cagaaacgca ttggttccca tcggcaccga gggcattcct tcgggactca catattccac
     3121 ggttcctgca aatcccactg gcggtttgcg ttcaccagtc ccaggatcct gcgaggaaaa
     3181 gtctatggat tgtatcggca agactgaact ggaaatcggt ttcttccctg tgatgtccat
     3241 ttcgttcatc accaagacca gagcctcatc ctgcccaaac agttccatcg ttcccggttt
     3301 tttctcaaaa atgatgtgat aagtcagctc ctgatcctgg gaaaaaggaa tggaaagttc
     3361 gggcggcgct tgtaatccga tttgaatgaa ttggacggca tgtatgcggg acaccaccac
     3421 tcgctgtggc ccctctttct ttcggaatgc caaagtgacc gcattatcat tggaaacctg
     3481 catcgtgacg accgtctcgt cggtcagtac gataccatcg agttgccccg caacggcctc
     3541 ctgatgatcc gtgggttcaa tggtgatttc cgaggccatg ccagagctgc ccacatcaaa
     3601 ttgcacggtt cgcccattga cggggatatc gaaccaggct ttcggcggat aactatccga
     3661 ctccatatca tagtcagccg gatccgcaac caacagccgg tctggactga tagcgagttt
     3721 cccgatattt tcaacggtca gggtctcaaa gggcaccgaa tccgtcaatc gtttatcatc
     3781 cacgcccggc gccacgctaa attccatccg ggtggtttcc acatgcaccg aaatatccgg
     3841 atcgactcgc acaaagtaac tgccgatcag caggatggca agaacccctc ccacaccggc
     3901 cttgaccccc acaagagcga ccaattttcc gagcagagaa ggtttggtag aagaggcctc
     3961 agggagctca ataggctttc cctctaattt ggcacggatg acagagagga gaatttcaaa
     4021 ttccttggat ccggccgtgc cttcccaccc aagaaagcac aaggcctgaa taccggtaaa
     4081 cgccaatggg atggcattgg cctccaacat gaccgggaca accttttcag tattcagggc
     4141 ttcccgagcc tctgcccgca cccaccggga acctttagcg tgagcggtcc ataacacgac
     4201 gacacatttc gccgaggtca acgcgtcttc aatcgtttta tcccactcgc ttcccacttg
     4261 aatctggcgg tcccaccaaa ccgacagccc cgattgctcc aaggacgcag ccaattgttg
     4321 ggccacgggt cgatcctcgc tggcgtaact gatgaacacg tccttcatgg ctgaagggga
     4381 acctcctttt acgggttcac gattggctga tggtagaaat gaatctcttt ggaaatgggt
     4441 tg
//
LOCUS       DATIAL010000568         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_425705, whole genome shotgun sequence.
ACCESSION   DATIAL010000568 DATIAL010000000
VERSION     DATIAL010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425705"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..874
                     /locus_tag="VLA60_17340"
     CDS             272..874
                     /locus_tag="VLA60_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HSF11179.1"
                     /translation="MKSKFHAGTSSQLSHRHLVLHKPYGVLSKFTDSEGRPTLADYVA
                     EARVYPAGRLDMDSEGLLLLTSDGDLAHRLTSPFQKVFKTYLVQVERIPDDPALVGLR
                     EGVIVKGKRTRPAKVQLLNEEPDVYPRPVPIRFRKSVPTAWLRMEIQEGMNRQIRHMT
                     AQVGHPTLRIIRVAIGPIRLGSLAPGQWRELRTGELRGLS"
     gene            1370..3511
                     /locus_tag="VLA60_17345"
     CDS             1370..3511
                     /locus_tag="VLA60_17345"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HSF11180.1"
                     /translation="MSVTIILALSVLGLAVAYYYSSSVLKIPIDMGVEDPETRKRLAK
                     IHSAIATGAMAFLKQEYKIMAIFMVVFAAIIAVLIDDHHTDYVNEGLYTAIAFIFGAI
                     ISIASGFIGMKIATQGNVRTTVSANQSLSSAFNVALHSGSVMGFALVSLAVLGLLIIY
                     LGLKSIMPAQLPTHILMEVIAGFGLGGSSIALFARVGGGIFTKAADVGADLVGKVEAG
                     IPEDDPRNPAVIADNVGDNVGDVAGMGADLFGSCAESTCAAMVISAVAFSGMVDALLY
                     PILISAIGIPVSLLTLMMIRVNSEDQVGPALKKMLIISSALMAVVMLFVTKAMLPDTF
                     EIGGQAYTDLGVYFCFLSGLIAGLAVGLMTEYYTSHDYGPVREVAKSCETGAATNIIF
                     GLALGYKSAVGPYLAIGLAIYIPWMLAGMYGVAIASLGMLGTLVIALTIDAYGPVSDN
                     AGGIAEMAGMPEHVRKRTDVLDAAGNTTAAIGKGFAIGAAILTSLALFAAFLTRADLL
                     LKEQHGPDFDLLGSINLLDPLVYTGLFIGAVLPAYFSAMTMKSVGSAAYKMIEEVRRQ
                     FRTIPGLMEGKAEADYEQCVAISTQAALKEMIAPGILIMGTPLITGFLFGIQAVAGVL
                     AGSLVAGGVIAISSSNSGGAWDNAKKYIEAGNLGGKGTDTHKAAVVGDTVGDPLKDTS
                     GPSLNILIKLSAILSLVFVPFFVQYGGLLIK"
     gene            complement(3670..3900)
                     /locus_tag="VLA60_17350"
     CDS             complement(3670..3900)
                     /locus_tag="VLA60_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11181.1"
                     /translation="MRKTGVPHGASHFHQNRDLALKNFKTSIFVVMRPKNEGKNRWND
                     HLGQPPQRKEKGDEERDKNCTRNGKMTNTTSR"
BASE COUNT          884 a          936 c         1168 g         1220 t
ORIGIN      
        1 attgtatggt ctgatctttt cttatgatat ttggagtgaa aggaggctga gttgatcggt
       61 aagagacctc catacggtcg tcaggttgga ggcggttctc acttgctcat taattagtgg
      121 ttgatgttga tcgaatagcc gcagagggct tggtaatatg gcagcgatga gataggttta
      181 attttttagg agggatgttt atgacgacat ggagaaaact attagttgtc tgtttgagag
      241 acgttttttg attgaccggc cgcgcagact aatgaagtca aaatttcacg ctgggacatc
      301 ctcgcaatta tctcatcgac atttggtgct gcataaacct tacggggtcc tgtcaaaatt
      361 taccgattct gaagggcgtc cgacattagc cgactatgtg gctgaggcac gggtgtatcc
      421 ggccggtcga ttggatatgg atagtgaggg cctgctgctg ctgacttccg atggagatct
      481 ggctcatcgt ctgacctcgc catttcaaaa agttttcaaa acctatctgg tgcaagtgga
      541 acgtattccg gatgacccag ctctcgtggg gcttcgggaa ggcgtcatcg tcaagggcaa
      601 aaggacccgc ccggctaagg tgcagcttct taatgaggag ccagacgtct atcctcgtcc
      661 ggtccctatt cgttttcgca aatccgttcc tacggcatgg ttgcggatgg aaattcagga
      721 aggcatgaac cggcaaattc gacatatgac tgctcaagtg ggacatccga cactaaggat
      781 tattcgtgtc gccatcgggc cgattcggtt gggctctctt gcgccaggac aatggaggga
      841 gcttcgaaca ggagaattgc gcgggttgtc ttagggcttt ctgggtcgcg tgtgtggaca
      901 ggcaaaagcc attcggatga aataattggc aggagcctga aacatctctc atgtttgcct
      961 tgcctttggt catggtgatg gtgtgggtaa taatagttat tagggttttt accttgaaca
     1021 ttgttgcccg gacaggtaaa attcagcgct cgtcatctaa tgaacatgag ttgatgaccg
     1081 gcggaacggc ggcatgacac atttttaaat cagaccgatg tcatgatggt gtctggtcgg
     1141 tccagggcaa cccctcgcgc gctgttttgt ccccaagcta gaacgataga aggaagttaa
     1201 ttcgagaatg aggcatttct ctgtggacga atagacggtc gggtcttagg ttgtgccgta
     1261 tggttttagc ccatagccgg aattcgacgt tctcatctta tagttcctgt tgagccggtg
     1321 cgaacaattc actattggtc acttacataa gaatggagat attcctaaca tgtcggtaac
     1381 tattattctg gctctgtctg ttcttgggtt ggctgttgcg tattactatt cttcatcggt
     1441 cctgaagatc cccattgata tgggtgtcga agatccggaa acccggaagc gtctggcgaa
     1501 aatccattcg gctattgcca ccggggccat ggcattcctg aaacaggaat acaaaattat
     1561 ggccatcttc atggtggttt ttgccgcaat tattgcggtg ttaattgatg atcatcacac
     1621 cgactacgtg aatgaagggc tgtacacggc tattgcgttc atttttggcg ccatcatttc
     1681 gattgcctcg ggtttcatcg ggatgaagat cgccacgcaa ggaaatgttc gaacgaccgt
     1741 ctctgccaac caatccctct cctccgcatt taatgttgcc ctgcattccg gctccgtgat
     1801 ggggtttgca ttagtcagtt tggcggtctt aggactgtta attatctatc ttgggctcaa
     1861 aagtatcatg ccggctcaac tcccgacgca tattttgatg gaagtcatcg ccggcttcgg
     1921 tctcgggggt tcgtctattg cgttatttgc tcgtgtgggt ggcggtattt tcacaaaagc
     1981 tgccgatgtc ggggctgatt tggtgggaaa agttgaagcg gggattccgg aagacgaccc
     2041 gcgtaacccc gcggtgattg ccgataatgt gggcgataac gtgggtgatg tagccggaat
     2101 gggggccgat ctctttggtt cttgtgcgga aagcacctgt gctgcgatgg tgattagcgc
     2161 ggtcgccttt tccggaatgg ttgacgcgtt gttatatccg atccttattt ctgccatcgg
     2221 catccccgtg agccttctca ccctgatgat gatcagggtc aattccgaag accaggttgg
     2281 tcctgcgttg aaaaagatgc tgattatctc cagtgcgctg atggccgtgg tgatgttgtt
     2341 tgtcaccaag gctatgcttc cggatacttt tgagattggt gggcaggcat ataccgatct
     2401 gggtgtgtat ttttgcttcc tgtcaggctt gattgcgggg ctcgccgtgg gattgatgac
     2461 tgagtactac acttcccatg actatgggcc tgtgcgggaa gtagcgaaat catgtgaaac
     2521 gggtgcagcc acgaatatca tttttggact ggcactcggc tataagagtg ccgtgggccc
     2581 ttatctcgcc attggtcttg ccatctatat cccgtggatg ttggccggca tgtatggggt
     2641 ggcgattgcc tcccttggta tgttgggaac acttgtgatt gcactcacca ttgatgccta
     2701 tggaccggtt tctgataacg ccggggggat cgctgaaatg gccggtatgc cggaacacgt
     2761 tcgtaagcgg acggatgtgt tggatgctgc gggaaatacg actgctgcga ttggcaaggg
     2821 ctttgccatc ggtgcggcta tcctgacctc gttggccctg ttcgcggcgt ttctcacccg
     2881 ggcggactta ctgctgaagg aacagcatgg tcccgatttt gatctgttag gcagtattaa
     2941 tttattggat cccttagtgt ataccgggtt gtttatcggg gccgtgttgc ccgcctattt
     3001 ttccgccatg acgatgaaat cggtggggtc ggcggcctat aagatgatcg aagaagtccg
     3061 acggcagttc cgcacgatcc ccggcctcat ggaaggaaag gccgaggcgg attatgagca
     3121 atgtgtcgcc atttcaactc aagctgccct gaaagaaatg attgcaccag gcattctgat
     3181 catgggaacg ccgctgatca ctggatttct gtttggcatt caagcggtgg ctggtgtgct
     3241 tgccgggtcc cttgttgccg gtggggtcat cgccatctcc tcatcaaata gtggtggggc
     3301 ttgggataat gccaagaaat atattgaggc tggaaatctt ggggggaagg gtacggatac
     3361 ccacaaggcc gcagtcgttg gtgataccgt gggtgatccc ctgaaagaca cctcagggcc
     3421 atccttgaat atcctgatca aactttcagc tattctgtca ttggtgtttg tgccattttt
     3481 tgtccagtat ggggggctct tgataaagta atttccatag agcaggggcg cccaacagga
     3541 ccgcagttcc cttttgtttt gttgggccga atgctgtcat ccttgttata atgattgatg
     3601 tagatgatgg tgtctccatt ttaagttcaa tgccgaaact gaccttgcct gtgtatggtg
     3661 tcttcatttt tatcttgatg ttgtgtttgt catttttcca tttcttgtac agtttttatc
     3721 tctctcttca tccccttttt ctttcctttg cggtggctga ccaagatggt cattccatcg
     3781 gtttttcccc tcatttttcg gcctcattac aacaaagatt gatgttttaa aatttttaag
     3841 ggcgagatct ctgttttggt gaaaatgaga ggcgccatga ggcactcccg tttttctcat
     3901 tcgtggttgt cccggtactt ttagaatgtg agggacaacg ctttcagagg gacagggcga
     3961 tccctctctc gtggcaaatg tcctgcctag ttccggtagg caggcttcct aactgactga
     4021 gatggcgggc ggaaaaggag tcaccccgta tggagccacc caaacaacca ggggattttt
     4081 cttttcagag gaagagaaaa tctgaggcgg tttcctccac agatgtggat ctgttgttgc
     4141 aggtgatctc ccaggatctt cctgcttcac tggcaattat ccaggacgcc tgtcagacgc
     4201 tcaccatg
//
LOCUS       DATIAL010000569         2939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1500282, whole genome shotgun sequence.
ACCESSION   DATIAL010000569 DATIAL010000000
VERSION     DATIAL010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500282"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VLA60_17355"
     CDS             <1..512
                     /locus_tag="VLA60_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11182.1"
                     /translation="FLLRGSQFQGTPREQVAAMALGYDLFIRLNPDHFKSKLLLLNAS
                     IRGKASPAFQKNLERAEQDNMGIVFGVLRDAVKEGHLQLRKGVTIEEVTFGLWTGAFG
                     AYVLMFSEVNLFALGISDPRKAVWENIHALLDGFGWHPFFHELDWEETRNRILKEIFP
                     QECHQAVIV"
     gene            825..1157
                     /locus_tag="VLA60_17360"
     CDS             825..1157
                     /locus_tag="VLA60_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11183.1"
                     /translation="MPDLTKKSTARLGDPHRWEKRLFDVPLLQQTPPKYDFQWKHGPN
                     FSLKDPGKLPGIQLGVEMHSFQALETVIPEGTLPTEIHVNRDRLPLVLPPSVNGPDYN
                     GGFLRFTW"
     gene            1769..2803
                     /locus_tag="VLA60_17365"
     CDS             1769..2803
                     /locus_tag="VLA60_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HSF11184.1"
                     /translation="MNQEISFRRITRYVGFSLGAFLIVATGAWGTFALYFDGPQHDML
                     RTLLASGFGVASLGALVGYCTRRFRRIATGSYLSLFVVLVVWWSMIEPSNDRQWQPEV
                     AVLPYATFDGNLVTVHNIRNFDYRTETDFTPAYYDRTYDLTNLDSADLVAAYWMGPMI
                     AHIFLTFGFGDDHLAISIEARKEATEGYSSIRGFFKQYELIYIVGDERDLIRVRTNYR
                     KDPPEDVYLYPLAGSPENAKRVFLGYMHTINELRERPKFYNTLTANCTNVIWMHTRLN
                     PGHVSFSWKILLSGYTPEYLYEQGKVNTDLPFEELQTRSRLNERAVQADQATDFSRRI
                     RVGLPLPASQ"
BASE COUNT          702 a          736 c          722 g          779 t
ORIGIN      
        1 cgtttctctt gagaggttcc cagtttcagg gcactccgag ggaacaagtg gctgcgatgg
       61 ccttggggta cgatttgttc attcggctta atccggatca ttttaaatcc aaattactgc
      121 tcctgaatgc atccatcagg ggaaaggcct ctccggcttt ccaaaaaaac ctggaaaggg
      181 ccgagcagga taatatggga attgtcttcg gagttttacg ggacgccgtt aaagaaggcc
      241 accttcagct tcggaagggc gtgaccatcg aggaggtaac ctttggtttg tggacagggg
      301 cctttggggc ctatgtgctc atgttctcag aagtgaacct ctttgccctt ggaatttcag
      361 atccgagaaa ggcggtgtgg gaaaacattc atgccttatt ggatgggttc ggctggcatc
      421 cgttttttca cgagctggat tgggaggaaa ccaggaaccg cattctgaaa gaaatttttc
      481 cacaagagtg tcatcaagca gttatcgtct aggatggact atttttttgg gacagaatga
      541 ctaaaagtca aaaattgact aattattgaa gaacctatta ctcagacagc taaggccggg
      601 ctctcgcgtt ttttggacaa ctgagtgatc ccttggaaga aaagatgagg aaagggtaaa
      661 attaagaaag gagtgagatc atgaaccctg caaccagaac tgacgtttca aatacaatcc
      721 tactaactgt tttgtcgatg ttcttgtcgt acataggaat cccttcacat gcagaagcga
      781 tgttggtcgc cgggaatgtt gtaagcggaa aggaattcgg tcctatgcct gatctcacga
      841 aaaagagcac agccagatta ggtgatcccc acagatggga aaaacgattg ttcgacgtcc
      901 cgcttcttca gcagactcct ccgaaatacg actttcagtg gaaacacggt ccaaattttt
      961 ctctcaaaga tccagggaag ttgccgggaa ttcaattggg ggtcgaaatg cacagttttc
     1021 aggctttgga aacggtcatt cccgaaggaa cccttccgac agaaatacac gtgaaccgtg
     1081 accggttgcc tctggttctt cctccctccg tcaatggtcc tgactataac ggaggatttc
     1141 tccggttcac ctggtaacat ccgaaattaa tttgtattct tgggccactg gggcgaaata
     1201 aaggttcacg tctccgcttt ggtctaaccg gatggtgaag tctccagggg attcggcgaa
     1261 agccatgccc caccggtgat acggcagctt gacggttgaa gcactcatcc cgggtctgtt
     1321 gaaaaaagcc gccagcggcg ttctcgccat ttttccgtgc tcacgtactc aacgtacgct
     1381 ccgcgcctaa aaacggctgc ggccttgctg gacggacttt tttgaaccga cccggagcct
     1441 ttgatgagta atctaatcct gggcaaattt gtccttgaaa atcttaatta ttcaacactc
     1501 ccatccctga acctgacgaa ggaaattgcc gggacctgta atcacctctt aaacatactg
     1561 ccgattgaac caccaccctt ctggaacccg gcgccagaat cttttagttc cagatctgaa
     1621 tgcttttgaa aagatccaaa ttgttaattg ggattttgca cgtagtctgt ttttctgaat
     1681 cttctgtgtc attcgccact gtgacgccta ttttgtggta ggctccgcaa ggagccagaa
     1741 gaacaggacc ctctcaactc tgacacacat gaatcaggaa atttcctttc gccggataac
     1801 aaggtacgtt ggtttttccc tgggagcctt cctgattgtt gccactggag cctggggcac
     1861 cttcgcctta tattttgacg ggccgcaaca tgacatgctc cggacgctac tggcttccgg
     1921 ctttggggta gccagcctgg gggccttggt ggggtattgc acccggcgat ttcgacggat
     1981 agccaccggg tcctatctca gcctgtttgt cgtgctcgtt gtctggtgga gcatgatcga
     2041 gccgtccaat gatcgccagt ggcaaccgga agtggcagtg cttccttatg ccaccttcga
     2101 tggaaatttg gtcaccgtcc acaacatccg aaactttgac tatcgcaccg aaaccgactt
     2161 tacccccgcc tactatgatc gcacctacga tctcaccaac ctggattccg cggatctggt
     2221 ggctgcctat tggatgggtc cgatgatcgc gcatattttt ctcacgttcg ggttcggtga
     2281 tgaccactta gccatttcca ttgaggccag gaaggaagcc acggaaggct attcgtcaat
     2341 tcgaggattt tttaaacagt acgagctgat ctatattgtg ggtgacgaac gggatctgat
     2401 ccgggttcgg acgaattatc ggaaagatcc acccgaagac gtgtatctgt atccattggc
     2461 cgggtcacct gaaaacgcca aacgggtctt tctgggatac atgcacacca tcaacgaact
     2521 tcgggagcgg ccaaagttct ataatacgct caccgccaac tgcacgaacg tcatttggat
     2581 gcacacgcgt ttgaatcccg gccatgtgtc attttcctgg aagattcttt taagtggtta
     2641 cacgcctgag tacctttatg aacagggaaa agtaaacact gatcttccgt ttgaggaatt
     2701 gcagactcgt tcacgcctca atgagcgggc agttcaggcc gatcaggcta cggatttttc
     2761 ccggcgcatt cgtgtgggcc tccctcttcc tgcctctcag taaacatccc caacacataa
     2821 cacgcttctc cacgtgttca tctcccctca tgtttcaggc tcttgacgaa tcttttctct
     2881 ctagggcctt tgctttagtt catggttttg gatacagtat cgcccctcca ttcagcagg
//
LOCUS       DATIAL010000570         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_853963, whole genome shotgun sequence.
ACCESSION   DATIAL010000570 DATIAL010000000
VERSION     DATIAL010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853963"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VLA60_17370"
     CDS             <1..516
                     /locus_tag="VLA60_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HSF11185.1"
                     /translation="SSPAQGGVTLIELLVTLVIMFILASVALPIAKVSIKRSQELELR
                     HTLRTVRTAIDAFQQDWARDGTVRTGKLCVENVTTCQESTGVTGYPKTLETLLKVKLT
                     GEKAQLGEQSEIKRYLRKIPFDPITETTEWGLRCFQDEPDESRWCGEDVFDIYTTSEK
                     KAIDGTPYREW"
     gene            516..917
                     /locus_tag="VLA60_17375"
     CDS             516..917
                     /locus_tag="VLA60_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HSF11186.1"
                     /translation="MTHFVVIGKKGYTLLELMIVVAIVGILVSIAIPTFQHTAIKAKE
                     AALKQNLFTMRAVIDQFYADRKEYPATLEDLVEEKYLRAIPVDPFTHSSTTWTEIYEE
                     QEEGEDSPAGIFDVKSGSDGQALDGTPYTEW"
     gene            925..1857
                     /locus_tag="VLA60_17380"
     CDS             925..1857
                     /locus_tag="VLA60_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HSF11187.1"
                     /translation="MKCNHWTHMILSGTFILILGGCAQKPTKPVAVDSSIEYVSPTSH
                     QEVLLQEARIENENLRAEIGALKILMAKQAGELQSLRGHTQTIQHREQDQGLELQHIR
                     GELLDTQAERDRLRKHNMELEGQVASMPDTTQLVSDIQILNQSFQQIMTSMKQLSTDI
                     TLIKQELHKSSGKGQPLQTKLSIPKPPPSSGDRRLPDSQGRIVIQNGDSLWKLSQMYD
                     VSIAQLHEWNDLDSDVIMTGFKIQVTSPMASQPNAVNTSSHPSEPADSPAIPEALAEP
                     VQHESLPIQDNRVENAPEPKHILSLGNPHLPESP"
     gene            1857..3062
                     /locus_tag="VLA60_17385"
     CDS             1857..3062
                     /locus_tag="VLA60_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HSF11188.1"
                     /translation="MPRFHYKAARPDGTIIETDGDGETSQSLRSQLEAQGLLVLDLAG
                     SGARQIVSRKKRQRPLALRDFLIFNQEFLALLKAGLPMLRCFDLLAERNTQGGFQQAL
                     QSVREHIRGGSAISEAMTHHPIYFPELYQASLRAGEQAGNLPEVLSRYIAYLKMLIGV
                     REKVVKATIYPIFLLGFGLLVTMGLLFFVLPSFAEVYQESRVELPWATQLLIDLTHSA
                     TQWMIGLLIFMGIGGMVIYWWKNTPQGQWQIHWLFLHAPILGPLFLKNQVIRLARTLS
                     TILGGGIPLLSALQVTAKSMPNKVFAKALQSVIVHVREGTSLSAALKKENFLPRLTLE
                     MVEVGETTGSLEAMLFEVAEFHEGELDFQLSRLMTWIEPMFIILIGVILGGVVIALYL
                     PIFQMAGAV"
     gene            3093..>3374
                     /locus_tag="VLA60_17390"
     CDS             3093..>3374
                     /locus_tag="VLA60_17390"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11189.1"
                     /translation="MTTPVKRMPFEDLLVEKGLILEREKETLIETARSTNQTIPTILL
                     KRGTHTEEVIGQVLADQYGLPWNTLKEFRIPSPLMESIPVELMHRYEFVP"
BASE COUNT          909 a          855 c          811 g          799 t
ORIGIN      
        1 tcctcgccgg ctcaaggggg ggtcacctta atcgaactcc tggttacgtt ggtcattatg
       61 ttcatattag cctccgtggc cctacccatt gcgaaagtca gcattaaacg gtcgcaagag
      121 ttggagcttc gccacacctt acgaacggta cgaaccgcca ttgatgcgtt tcaacaggat
      181 tgggcgagag atgggaccgt ccgtacaggg aaactctgcg tggaaaacgt gacgacttgc
      241 caggagtcca cgggagtgac cggctaccct aagacgttgg aaactctctt gaaggttaaa
      301 ttaacaggtg agaaagccca actcggagag caatcggaaa ttaaacggta cctccggaaa
      361 atcccctttg acccaataac agagaccaca gaatgggggc tccggtgttt ccaggatgaa
      421 ccagacgaat cccggtggtg cggggaagat gtctttgaca tctataccac cagcgaaaaa
      481 aaggcaattg atgggacccc ctatcgtgaa tggtaatgac ccactttgta gttattggca
      541 aaaagggcta cactttactc gagctcatga ttgttgtggc aatcgtgggg attctggttt
      601 ccatcgcgat tcccacgttt caacacacgg caataaaagc caaggaagcg gctctgaagc
      661 aaaatttatt caccatgcga gcggtcattg atcaatttta tgcggacaga aaggaatatc
      721 cggcgacctt agaggacctg gtggaagaga aatatcttcg agccatcccc gtggatcctt
      781 ttacccactc ctcgaccact tggaccgaaa tttatgaaga acaggaggag ggcgaggact
      841 ctcctgctgg aatatttgat gttaaaagtg ggagtgacgg ccaggcgctg gacggcactc
      901 cctatacaga atggtaggta ggaaatgaaa tgtaatcatt ggacgcacat gattctgagc
      961 ggaacattta tcctgatttt aggcggctgt gctcaaaaac cgaccaaacc ggtcgcggtc
     1021 gattcctcca tagagtatgt ctccccgact tctcaccaag aggttctcct tcaagaagcc
     1081 aggatagaaa atgaaaattt acgggctgaa ataggggcat taaaaattct catggccaaa
     1141 caagcgggtg aactgcaatc gctgagaggc catacgcaaa ccattcaaca tcgcgaacag
     1201 gatcaagggc ttgagctgca acatattcgt ggagaacttc tggacactca ggccgagcgc
     1261 gatcgactgc gaaagcacaa catggaactt gagggccagg ttgccagcat gccggatacc
     1321 acgcagctcg tgtcagatat tcaaatcctc aaccaatcat ttcagcaaat tatgacgagc
     1381 atgaaacagc tttccactga tatcacgctg attaaacaag aactccacaa gtcctcaggt
     1441 aagggacaac ccctgcaaac gaaactctca atccccaaac caccaccctc ttccggggat
     1501 agacgcttgc cggattccca aggacggatc gtcattcaaa atggggactc cctctggaaa
     1561 ttatcccaaa tgtatgatgt ctccattgcc caattacacg aatggaatga cctcgattct
     1621 gacgtgatta tgaccggttt caaaattcag gtcacctccc cgatggcatc tcagccgaat
     1681 gcggtaaaca cttcgagtca tccaagtgag ccagcggatt ctcctgccat cccagaggcc
     1741 ctggctgaac cggtacaaca tgaaagcctt cccatacagg acaatcgtgt ggaaaatgcc
     1801 cctgagccca aacatatact ctcacttggc aatcctcatt tacctgagtc accctagtgc
     1861 cccggtttca ttacaaagcc gctcgtccgg atggaacgat tattgaaacc gatggggacg
     1921 gcgaaacatc tcaaagcctt cgctctcaac tcgaggctca aggactgctt gttcttgacc
     1981 tggcaggctc tggcgccagg cagatcgttt cacgaaaaaa acggcaacgt cctttagcgc
     2041 ttagagattt cctgattttt aatcaggaat ttttagccct gctcaaagcc gggttgccca
     2101 tgttgcggtg ctttgatctt ctggcagaac gaaacaccca gggggggttt caacaagctc
     2161 tgcaatccgt ccgggagcat attcgaggag gatcggccat ttccgaagcc atgacccatc
     2221 atccaatcta ctttccggaa ctctatcagg cttccttacg agccggggag caggcgggca
     2281 acctccctga agtcctaagc cgatatattg cgtatttaaa aatgctgatc ggagttcggg
     2341 aaaaagtcgt gaaagcgacc atttacccca tatttttatt ggggtttggt ctcctggtga
     2401 ccatgggtct gttatttttt gtgcttccgt catttgcgga agtctatcaa gaaagtcgcg
     2461 tggaattgcc ctgggccacc cagctcttaa ttgatctgac ccattccgcc actcaatgga
     2521 tgatcggact gctgattttc atggggattg ggggaatggt gatctattgg tggaaaaaca
     2581 cccctcaagg gcaatggcaa atacattggt tatttctgca tgctccgata cttgggccct
     2641 tgtttttaaa aaatcaagtg attcggttag ccagaacgct gtcaaccatt cttggaggcg
     2701 gaatcccgtt gttatccgcg ctgcaagtca cagcgaaatc catgcccaat aaagtctttg
     2761 ccaaagcgct ccaatcagtc attgttcatg ttcgggaagg taccagcctc tccgccgcat
     2821 taaaaaaaga aaactttctc cctcgcctca ccctggaaat ggttgaagtc ggtgaaacga
     2881 ccggttcact ggaagccatg ctttttgagg tcgccgaatt tcatgaagga gaattggatt
     2941 tccaattatc tcgactgatg acatggatcg aacctatgtt catcattctg attggcgtta
     3001 ttttaggagg ggtggtcatt gccctctatc ttcctatttt ccaaatggca ggagcggtgt
     3061 aacaaccaaa tgccctcgac gagagaagaa ccatgaccac accagttaaa cgcatgccat
     3121 tcgaggacct cctggtggaa aaggggctca tcctggaacg ggaaaaagag acgttgatcg
     3181 aaacggcccg atcaaccaat cagactattc ccaccatttt gttaaaacga ggcacccaca
     3241 cggaagaggt gatcggccag gtcctggctg atcaatatgg cttaccatgg aatacgctta
     3301 aagaatttcg tatcccctca ccgctcatgg agtccattcc ggtcgaactc atgcatcggt
     3361 atgaattcgt gccc
//
LOCUS       DATIAL010000571         2472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_746391, whole genome shotgun sequence.
ACCESSION   DATIAL010000571 DATIAL010000000
VERSION     DATIAL010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746391"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..386
                     /locus_tag="VLA60_17395"
     CDS             183..386
                     /locus_tag="VLA60_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HSF11190.1"
                     /translation="MDKQERKYESRKPSSPKETEDVQADPNVAETGQRLKEEMDELVD
                     EIDKVLEENAAEFVKNYVQKGGE"
     gene            728..>2472
                     /locus_tag="VLA60_17400"
     CDS             728..>2472
                     /locus_tag="VLA60_17400"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HSF11191.1"
                     /translation="MGVTHLTDIVQLMDQVKAYYPEADTDFIKRAYDYSAKAHEGQTR
                     RTGEPYVQHPLAVAGILTFLKLDVPAIVAGLLHDTLEDTVATRAELEKEFGSDVARLV
                     EGVTKIGQIPFKTDEEKQAENFRKMVLSMADDIRVVFIKLADRLHNMQTLEALSDEKR
                     KKIAQETMEIYAPLANRLGMSWIKQQLEDLCFQYLEPDAWSHLKLRLAKKDEERQEYI
                     QSLIHLAQQALLDAGLPGQIDGRPKHLFSIYQKMERRSVSFDEIFDLTAVRIVTDTKM
                     NCYAILGLLHSIWPPVPGRFKDYIATPRSNLYQSLHTTVVGPQGEYVEFQIRTADMHR
                     INEYGVAAHWLYKEPGKVGERDEQVFSWLRQFVEWHKDLADNRQFMDSVKLDLFHDVV
                     FVFTPTGEVREIPVGATPLDFAYAIHTEIGDHCVGAKINGKLVPLRYQLSSGDMVEIL
                     TSPSQVPHRGWLKFVRTSRAKTKIKHWFKVEEQKRALELGRRLLEQEFRRHNLPVAQS
                     LKSDRLSSMAKAKGSETVEELIRQVGFGRLSPDQIVSHFYEGAEQGSSSPTKGVPHSP
                     QQKLPGLLREGSVKFG"
BASE COUNT          652 a          520 c          652 g          648 t
ORIGIN      
        1 cctcttccgt gtgaaggaag cgctcttcca ctgagctaat cgcccgcggg aggcaattta
       61 tcataatgct aggaatggag caatgaagtc tgcgagggtg ataatggttg tcttgacggt
      121 aggagaaagt gtggagtagg ctgcgagtag gtgtttcatc gacttgacca aggaggtgca
      181 ccatggacaa gcaagagcgt aaatacgaat ctcgaaaacc gagctctccc aaagaaacag
      241 aggacgtaca ggctgatccg aatgttgcgg agaccggtca acgtctaaaa gaagaaatgg
      301 atgaactggt tgatgaaatt gataaagttt tagaagaaaa tgcggctgaa tttgtcaaaa
      361 attatgtgca aaaaggtgga gaataaatgc gataggttcg ttgatttcta gaaattctct
      421 cctgccctct ttcactcgcc agatatacta accccgttgc cgttccctgt tcttctcaga
      481 gtcattttca gtggaggtct tccggcctca caactttacg catcgtattt ttgctggttc
      541 agttcgatga aagtgcctct ggaatgaccg gtccttcatg gggtaatgga gcttcttcct
      601 tcttgtgcca cctcgggatg tggggcgccg attaccgctt ctggaaggaa aatgcggagc
      661 atttttctct attttttgtt atgattcgtg ggcaatggcc cgagacaatt ggatgaagga
      721 ctaaaaaatg ggcgtgacgc atttgaccga tattgtacaa cttatggatc aggtgaaagc
      781 ctattacccc gaggctgata ccgacttcat caaacgagcc tatgactatt ccgcgaaagc
      841 tcatgaaggg caaactcgac gaactggcga accctatgtc cagcatccat tagcggtcgc
      901 tgggatttta acttttttga aattggacgt ccccgcgatt gtcgctggac tattacatga
      961 tacgctcgaa gataccgtag cgacgagagc cgagttagaa aaagagtttg gaagtgacgt
     1021 ggctcgttta gtggagggcg tcacaaaaat tgggcaaatt ccttttaaaa ccgatgaaga
     1081 gaagcaggcg gaaaatttcc gtaaaatggt tctctctatg gctgatgata ttcgagtggt
     1141 gtttatcaag ttagcggatc gattgcataa catgcagaca cttgaggcgt tgtctgacga
     1201 aaaacggaaa aaaattgcac aagaaaccat ggaaatttat gcgccgctag ccaaccggct
     1261 tggtatgagt tggattaagc agcagctaga ggatttgtgc ttccaatatt tggaaccgga
     1321 cgcctggtct cacttaaagc tacggttggc taaaaaggat gaggagcgcc aggaatatat
     1381 tcaatccctg attcacctgg ctcagcaagc tcttttggac gcagggctgc cgggacagat
     1441 cgatggacgt cccaagcatc tctttagcat ctatcaaaaa atggagcgcc ggtccgtctc
     1501 ttttgacgaa atctttgatt tgacggcggt tcgtattgtg acggatacca aaatgaattg
     1561 ttatgccatt ttgggattgc tgcattccat ttggcctccc gtacctggtc gctttaagga
     1621 ctacatcgcg actccacgtt cgaacttgta tcaatcgtta cacacgacgg ttgttggccc
     1681 acaaggagag tatgtggaat tccaaatccg gacggcggat atgcatcgaa ttaatgaata
     1741 cggtgtggca gcccattggc tctataagga gcctggaaaa gttggcgaac gggacgaaca
     1801 ggtcttcagc tggctcaggc agtttgtgga atggcataag gatcttgccg ataaccgcca
     1861 gtttatggat tcggtcaaat tggatttatt tcatgatgtt gtgtttgtgt ttacgccaac
     1921 aggggaagtc cgggaaatcc ctgtcggggc cactccatta gactttgcat atgcgattca
     1981 tacggaaatc ggagatcatt gtgtcggggc aaagattaat ggaaaattag ttcctctccg
     2041 ttatcaatta agcagtgggg atatggtaga gattctgact tccccctcgc aggtacctca
     2101 tcggggttgg ttgaaatttg tccgaacttc ccgggccaaa acgaaaatca agcattggtt
     2161 taaagttgaa gagcaaaagc gggccctgga acttgggcga cggctgttgg aacaagaatt
     2221 tcggagacat aatcttcctg tcgcacaatc gttgaaatct gatcgccttt catcaatggc
     2281 taaggcaaag ggcagtgaaa cggtggaaga attgattcgg caggttggat tcggccgttt
     2341 gtctccggat caaattgtca gtcactttta cgaaggggct gaacaagggt cttcgtctcc
     2401 cacgaagggc gtccctcact ctcctcagca aaagctccct ggactccttc gggagggatc
     2461 tgtgaagttt gg
//
LOCUS       DATIAL010000572        12258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_426030, whole genome shotgun sequence.
ACCESSION   DATIAL010000572 DATIAL010000000
VERSION     DATIAL010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 12258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12258
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426030"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VLA60_17405"
     CDS             complement(<1..1259)
                     /locus_tag="VLA60_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HSF11192.1"
                     /translation="MKTAEHLRLEEARQEKMLWKKWGPYLSERQWGTVREDYSEGGDA
                     WNFFTHDHARSRAYRWGEDGIGGISDEQQRLCFALALWNGKDPILKERLFGLTNSEGN
                     HGEDVKEYYFYLDSTPTHSYMKYLYKYPQRAFPYADLVDINRKRSRNEMEYELLDTGV
                     FNDDRYFDVFLEYAKDGPDDILMQITAVNRGTEEAELHLLPTLWFRNDWASWIAPSNR
                     AAEQPTLKQIQAMADTSAIVAQHSTLGTYYLYCEGDVPLLFTENTTNNDRLFPGQPNA
                     GPYVKDGINNYLIQGHQSAVNPEHQGTKASAHYRQRVGPGQSMTVRLRLTNQTSTVPF
                     GPAFAKTLAARLQEADEFYHSITPPSVSPDAAHVMRQALAGMLWSKQFFFFDGDNWLD
                     EHQSNPLHSGYRNSRNSEWFHMVNKDI"
     gene            2499..4262
                     /locus_tag="VLA60_17410"
     CDS             2499..4262
                     /locus_tag="VLA60_17410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HSF11193.1"
                     /translation="MTEETRTAGKFAGGGPVRGWREAFPPAEWLVSYRPQWMAQDAIA
                     GVTLAAYGIPVSLAYASLAGLPPQYGIYCYLVGGLFYALFGSSRQLAIGPTSAISMLV
                     GVTVAGMAQGDPGRWADIAALTALVMSGMCLIAWLLRLSSLVNFISETILLGFKAGAA
                     LTIALTQLPKLFGVKGGGEHFFERLVILGGQLPDTNLAVLAFGLAALAALLVGEKLLP
                     GFPVALCVVVISIIALSVAPLGALGFNVVGALPQGLPDFHPPGLRLRDVDGVIPLAFA
                     CLLLAYVESVSAARALAQANGYEINPRQELLGLGAANLASAFFQAYPVAGGLSQSSVN
                     DKAGARTPLALVFASATIGLCLIYLTGLMSNLPTVVLAAIVLFAVKGLIDIDELRHVW
                     RVSRFEFNISMVAFAGVLLLGILKGVMVAVVISILLLIRRAAQPHVAFLGRIPGTRSY
                     SDIGRNPDNEAVPGVLVFRVEASLLYFNAEHVRDAVWQKIRSTTGDLKLVICDLSTSP
                     IVDLAGVRMLATLHATLEEMGIRLRLVSPHAAVRDILRAEGLEERVGDFGHGVMVADA
                     IDELQGCSGAVSQVVQIPPAT"
     gene            4536..8573
                     /locus_tag="VLA60_17415"
     CDS             4536..8573
                     /locus_tag="VLA60_17415"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HSF11194.1"
                     /translation="MFRPSRKFWISLVLIFGVCGVLAYELFPFIKTDVYRETLEMGFS
                     ASLGRKVSLEGPLSLTFSSQPHLILEHVSVSNPPWTSQPHLFRADRVEIGLSLWPLLQ
                     RRLEVEKIALEGAKLLLEEGSEGLDNWTLQEDSRPGFLSRAVPSVFMTISERGEIIIE
                     RSQIQYRSYPAEDTTEVSIHRAVVIAPDDQYRKFSFEGTYHDSPVNIELIGGRIMDLF
                     TLTEPWPVDGVLSTTGAFAAIKGRVGGANSDQMFALQIQVNGDRLSALNDLLKIGLPD
                     SAPFMIAANVVKTPDVLNLSDIHGTLGASDVAGQLRVQNEDGRQKMSGRLTSNAVQLH
                     DLTSLAHHHGSKPAPSLEDTASVSPASPPVEVDLDVTVAKLLFGETDLGSLSFTAGIR
                     EGLVLLDPIHIKSFGGTGNARLTFDYKPSQAQSTLQAKVRSLNYGSALRALGGAMNIE
                     GSTDFDLTASGSGVLLPELFKSLTVNLQNRRTTFGFSDPMPEAKTSVVLREGSLRVTK
                     GGPVTMVAQGTYRERAVHLKLATTSLIELATPETTWPLSLTARGGGAFLEAKGFLQTG
                     GPDLDGALTVALNGRRLSELDPDLPPVGPYALRAQVIKKGDRYEVSDFRSRFGNSDFS
                     GILEMNFQKARPYLTGIFTSKHIEWTELSGPENEGESAIPSEGMRAIDVDLNMVINHV
                     RNGRLNVAGLTFTAGLQAGRLKVESVQGTVLDQQSAYGNFHGGFQLDTTRPIPTISGN
                     VSLDHVRYEHLFPEAGFVDLAENVVNLNAEFSGAGHTISDMLNHSTVRVDGEKLSVRF
                     HREADHPASVQVMANLKVETVDGGPLRLYVEGVFDTTSFRLRSSTGSMMGLMKDRGLW
                     PMNVRLDLPEAVVEVSGHLTLPHPAQEFTLQVLVKGNNLRDLNFLTAASLPDAGPLDI
                     TGLVTRSPVGYHVTDLKGVLAGSDVQGHVMVLTKGIRPRVRGKLTANNLVLGAVKQPL
                     DDSSAQQKRSTLKAFGDEVRGIGSSAVNVVRDTIGMRKDSGALPLKSIPDWVFPIEDL
                     RAIDLMLDAEIKHIRRAEEDLGHASFQIALEEGLLTLHPLTGNLWGGDFEGKFVLDGT
                     KYVPTLDVELHIHGLNYGRVARYFGGSDLVKGKSQSILLALQGRGDTLHEVLEQASGR
                     FELVDGPLELATKYIDLWAADLITTALTTAWKSESVTKLNCTVGYFDIEEGVVKSDDI
                     LIDSHRLTIAGIGKLNLADETLDIVLTPRPKDPSLFSLAHTVRITGPLSNPNVSSDKL
                     RIAESGGWGLLGLVTPMGWVIAIPQIAGTTVGTMNQNPCVEALKGRPQTAQALDEIKG
                     GLWGKIKRAFSNLAGFSETPSANPQ"
     gene            9018..9491
                     /locus_tag="VLA60_17420"
     CDS             9018..9491
                     /locus_tag="VLA60_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11195.1"
                     /translation="MRFPIQTLQVLKVAVMIAILISFPKTGLGITKAITTPPPVSGHE
                     LSIESITPADVFARVQLIRNELDDLRFEMGRPKSHEIGLLVENATPHEVFFQAKTLNQ
                     KVARLVTELTEESEVGGSEEDPVDIRPYHVWKMIDSSLHRILSLNSLLSGNSDSA"
     gene            9554..10042
                     /locus_tag="VLA60_17425"
     CDS             9554..10042
                     /locus_tag="VLA60_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11196.1"
                     /translation="MANTQLNLLLMHQFFPKDAAELVGLGIEYTTQLLRQFPTAPVPA
                     PLPSLERGRKSWNSYDHLIDCYMVLGKIARASNIKMLTLAPQNLDRPDIQSNDVYDLA
                     TLVISELAYLHAHLPHIPPPSPVRLRGPVLPSHVYQQVGGLLAQLKVLQMQVQSHPEW
                     LK"
     gene            complement(10666..>12258)
                     /locus_tag="VLA60_17430"
     CDS             complement(10666..>12258)
                     /locus_tag="VLA60_17430"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HSF11197.1"
                     /translation="VLPPILVPTAPVILDGETLFQVRGVSALPAEERARRIIERIDEF
                     GSDLSLSPQSLRLVEEKGVTMIFAGDQPLAGVTDADGRLEGGVERGILARIYLKRIEE
                     AVVAHRETRKPINILIHIAFSLLATAILASAWWGTRWVFDKLDAVIERRYKERVKDVT
                     VQKVIILQAHQMWAGLRGSLNGIRTFLLFLLLYAYLNFVFRQFPWTAYIGKQLFDIIL
                     DPIRVIVKGFLSSFPSLVFLVILVFLLRYLLKFGKLIFANLAIGKVTWPGFDREWAWP
                     TYRIFRTLVIAFGIVVGYPYIPGSTSEAFKGITIFLGVLLSLGSSSIIGNILAGYTLV
                     YRRAFQEGDRIKVNEILGDVIERRILTTRLRSLKNEEIIVPNSEILNSHVTNYSAAAR
                     ENGLILHITVGIGYETPWRQVEAMLIEAADRTPGLLKDPPPFVLQLALGDFCVTYELN
                     VYCDRPYYMLELYTGLSQNILDVFNEHGVQIMTPAYITDPAQPKIVPKDHWYTPPAKP
                     PKKKEKQEVESAPDTPPSEKNI"
BASE COUNT         2738 a         3169 c         3344 g         3007 t
ORIGIN      
        1 atgatgtcct tgttgaccat gtggaaccat tccgagttcc gggagtttcg atatccggaa
       61 tggagggggt tggactggtg ctcgtcaagc cagttgtctc catcgaagaa aaagaactgc
      121 ttggaccaga gcatgccggc gagggcctgg cgcatcacgt gcgccgcgtc cggggagacg
      181 gacggcgggg tgatggagtg atagaactcg tctgcctcct gcaggcgagc tgccagggtc
      241 ttggcaaacg ccggcccgaa agggacagtg gaggtctggt tcgtgaggcg caatcgcacg
      301 gtcatcgact ggccaggccc caccctctgc cggtagtgcg ccgatgcctt cgtcccctga
      361 tgttcagggt tcacggcgct ctgatgaccc tgaattagat agttgttgat accgtccttg
      421 acgtacggtc cggcattcgg ttgtccagga aaaagcctgt cgttgttcgt ggtgttttcg
      481 gtaaagagca gcggtacatc gccttcgcag tagaggtagt aggtgcctag tgtagagtgc
      541 tgcgccacga tggcactcgt gtccgccatt gcctggatct gcttgagggt gggttgctcg
      601 gccgctctgt tggatggggc aatccacgag gcccagtcgt tgcggaacca caaggtcggc
      661 aacaggtgaa gttctgcctc ctccgtcccc cggttgaccg cggtgatctg catcaggatg
      721 tcgtccgggc cgtctttggc atactccaga aaaacatcaa agtagcgatc gtcattgaag
      781 acgccggtgt caagcagttc gtactccatc tcattcctgg aacgcttgcg gtttatgtcc
      841 accagatcgg catagggaaa ggcccgttgt ggatacttgt aaagatattt catgtaggag
      901 tgcgtcggcg tgctgtcgag gtagaaataa tattccttga cgtcctcgcc atggttgcct
      961 tcgctattcg tgagcccgaa gagccgttct ttcaggatcg ggtctttccc attccagaga
     1021 gcgagcgcaa agcagaggcg ctgttgctca tcggagatcc ccccgatgcc gtcttccccc
     1081 cagcgatagg cgcgcgaacg tgcgtggtca tgggtgaaga aattccaggc gtccccgcct
     1141 tcgctgtagt cctcgcgcac cgtcccccac tggcgttcgc tcaagtaggg accccatttc
     1201 ttccaaagca ttttctcctg acgtgcttct tcgagccgga gatgttcagc agtcttcata
     1261 gaactcctgt ctcacttcta taactcacat cacaaggaac gggcatgtat aagttacata
     1321 tgtccgagac cgtctcacac tccatggtcc ccttgtctca acgcatcggc acttgcctgc
     1381 cgacaaaatc caacaggctc aaagcaaaat caatgccttg atgcccttca tgatagcttc
     1441 cttacatttg ggaagggtgg tccaacttta tacgaaacga ccctcctccg ctggtggaaa
     1501 aagatgccaa gatctgagta cccgcttcat ggcgactctt cagtccatcg ggtatttcca
     1561 tcagaattct ctctttattg cttggacaaa tacaaattaa tgatttgttc cagcaagaac
     1621 tatgcgcacg gtttggtgta tgacccgcac aaatatatgg cgtgtaaatt caatacttta
     1681 catactaagg gaatcgacga cggggatagg atgtatacag gagtggggag gaatgcgcca
     1741 gagggcgggc aggcagaaaa gagaaaatgc cacacgcaga attaagtttt cctcttgtcc
     1801 acttgcgcca ggaggccatg cccgtgtcta tggcctaggc acccctttct atccatcgtt
     1861 catctcagtg accttggcag gttctgcacc ggggagaggt acagtaggcc atacagtccg
     1921 tcttacacgg gacagtctgt cttttatggg aacaatatgt catggggtgg attcgggtga
     1981 cgagtgttct gccttactga tttacaatgt gtattgggaa tccatcgtgg ggtttgccgg
     2041 taaaaacgcg ttggtaggtg aacgtgtcgc atgtgtgtac cccattcccc tgtcaatgtg
     2101 attttctgtg atgctggttt tcacccccca gcagccgaga ttttgcctgg cgcgtccgga
     2161 agggcgtaaa ctttatatgg agcagaaggt cacaaaccca ttgacgcccc gcctcattgg
     2221 aggtgactaa cgcctgcctg tgaatcatca cttccttcag cgggtcgaag tatgatctga
     2281 gtcaacatca agacctaaac ccaagcgcat gcgatcgctc aatttgctgg atcggtcggt
     2341 gaatggggac ttctccttga tcctgaagga caagtttata acttctcaaa accggggctt
     2401 tgtatgctat gcttcgtgcc cgtcaacatt ttcatctgcc tggacaggcg caccagcgga
     2461 cgggatttcg aagcagatat gtcaatggac acgacgtcat gactgaagaa acccgaaccg
     2521 ccggcaaatt cgcgggcgga ggtccggttc gagggtggcg ggaggcgttt cccccggcag
     2581 agtggctggt ttcgtatcgg ccgcagtgga tggcacagga tgccattgcc ggcgtgaccc
     2641 tcgctgccta tggcattccg gtgtcactcg cctatgcgtc gctcgccggg ttgccgccgc
     2701 aatacggcat ttattgctat ctggtcggcg gcctgttcta tgcgctgttt ggttcttcgc
     2761 gccagcttgc gatcgggccc acctccgcga tctcgatgct cgtcggggtc accgtcgccg
     2821 gcatggcaca gggcgaccct gggcgttggg cggacattgc cgccctgaca gcgctggtca
     2881 tgtccggcat gtgcctaatc gcctggctgc tgcgattgag ttccctggtc aacttcatca
     2941 gcgaaaccat cctgctcggg ttcaaagccg gcgcggcact cacgatcgcc ttgacccagt
     3001 tgcccaagtt gttcggggtc aagggtggcg gggagcactt cttcgagcgt ctcgtcatcc
     3061 tgggcggcca gctccccgat accaacctcg ccgtgttggc tttcggtctc gccgcgctcg
     3121 ccgcgctgtt ggtgggcgaa aagctgctcc cgggtttccc tgtcgcgctg tgcgtggtgg
     3181 tgatttccat tattgcccta tcggtcgctc cgctgggtgc tcttggcttc aacgtcgtcg
     3241 gcgcgctgcc tcagggcttg cccgacttcc acccgccggg tctgcggctg cgcgatgtgg
     3301 atggcgtgat tccgctggcg ttcgcgtgcc tattgctcgc atacgtggaa agtgtttccg
     3361 cagcacgtgc tctggcgcag gcgaacggct acgaaattaa tccccgccag gaattgctcg
     3421 gcctgggtgc cgcaaatcta gcctcggcgt tcttccaggc ctacccggtg gcgggtggcc
     3481 tgtcgcagtc ctcggtgaac gacaaggccg gggccagaac accccttgcg ctggtgttcg
     3541 catccgcgac catcggcctg tgtctgatct atctgacggg attgatgagc aacctgccca
     3601 ccgtcgtgct cgcggccatc gtgctgtttg cggtcaaggg attgatcgac atcgacgaat
     3661 tgaggcacgt gtggcgggta agccgtttcg aattcaacat atcgatggtc gcgtttgccg
     3721 gagtgctgct gctgggcatc ctgaaaggcg tcatggtggc ggtcgtcatc tcaatattgt
     3781 tgctcatccg ccgcgccgcg caaccgcatg ttgccttcct cggccgcatt cccggcaccc
     3841 ggagttactc cgacatcggg cgcaatccgg acaacgaggc cgttccgggt gtactggtgt
     3901 ttcgtgtgga ggcgtcgctt ttgtacttca atgctgagca cgtgcgtgat gccgtctggc
     3961 agaaaattcg ttcaaccacc ggagatctga agctggtgat atgcgatctg tccacttcac
     4021 ccatcgtcga cctggccggt gtgcgcatgc tggcaaccct gcacgcgaca ctcgaggaaa
     4081 tggggatccg tcttcggctc gtttcgccgc acgcagcggt gcgcgatatc ctgcgcgccg
     4141 aaggattgga ggaacgtgtc ggcgacttcg gccatggggt catggtggct gatgccatcg
     4201 acgagttaca gggttgctca ggagcggtga gccaggttgt ccagatcccg ccggctacgt
     4261 agcatccacc cccggtcttc cgcacccagc gcccacgtgg cagagcctgc taatgggtga
     4321 ccccctgctt cccctgtttt ggcgggtcgg cgggaattat ttgacgccgt cctcagtatt
     4381 ccctgggagg tttccgcttc acttggttta ttttttcttt gagagaaagg ggcgtcatct
     4441 ttttttgcta gccttcacct ccgctcaaaa ccaaatttgg gattatccgg atggaaatga
     4501 tagcaggggc cctcatatta tagaataaaa gatatatgtt ccggccctca cgaaaatttt
     4561 ggatcagcct tgttcttatt tttggagtct gtggcgttct cgcctatgaa ttattcccgt
     4621 ttatcaaaac cgatgtgtat cgggaaacac ttgaaatggg attctcggcg agcctgggga
     4681 gaaaggtttc actggaagga cctctctccc tgaccttttc ctcccaacca catttaattc
     4741 tagaacacgt ttctgtgtcc aaccctccct ggacttctca accacatcta tttcgagccg
     4801 accgcgttga gatcggattg tccttgtggc cgcttcttca acgtcgtttg gaggttgaaa
     4861 aaattgctct tgaaggcgca aaacttcttt tagaagaagg gtccgaaggt ctcgacaact
     4921 ggaccctcca ggaagacagc cggcccggct tcctgtcccg ggccgtgccc tcggtcttca
     4981 tgacaatttc tgaaagaggg gagatcatca tcgaacgctc ccaaatccag taccggtcat
     5041 accctgctga ggatacgaca gaagtgtcga tacatcgggc ggtggtgatt gcccctgacg
     5101 atcagtacag aaaattttcc tttgaaggta cctaccacga tagccctgtc aacattgagt
     5161 taattggtgg tcgtatcatg gatttgttca ccttgactga accctggccg gtggatggcg
     5221 tgctgtctac aacgggggca tttgctgcta ttaaaggaag agtcggaggg gcgaattccg
     5281 accagatgtt tgccctccag atccaggtga acggagaccg tctaagtgcg ctgaatgatc
     5341 ttctgaagat cggccttccc gattccgccc cgttcatgat cgcagccaat gtcgtgaaga
     5401 ctcccgatgt cctcaatttg agtgatatcc atggaacatt gggtgcctcg gatgttgccg
     5461 gacaattgcg ggtacagaat gaagatggac gtcagaaaat gagcggtcga ttgacctcca
     5521 atgccgtgca gctacatgat ttgacctctc ttgcacatca ccacggatcc aagccggcac
     5581 cttcccttga agatacggcg tccgtttctc ccgcgtctcc gcctgtggag gtcgatttgg
     5641 atgtcacggt cgcaaagctc ttattcggcg aaacggatct tggttcgctg tcttttactg
     5701 ccggcatacg ggaagggctg gttctgctcg accctattca cattaaaagt tttggaggaa
     5761 cggggaatgc ccggttaacc tttgattaca agccttctca ggctcaatcc acgcttcaag
     5821 cgaaggtgag atccttgaac tatggatctg ctcttcgggc tcttggggga gccatgaata
     5881 tcgaaggctc tacagacttt gatctcaccg ctagcgggag tggtgtcctg ttgccggaac
     5941 tcttcaagtc cctgacggtg aatcttcaaa accgtcgcac caccttcggg ttttccgatc
     6001 caatgcctga agccaaaacg tctgttgtct tgcgagaggg atcccttcgt gtgacgaaag
     6061 gcggaccagt cacaatggtt gcgcaaggca cctatcgaga aagggctgtt catctgaaac
     6121 tggctacaac ctctttgatt gagctggcca cgccggagac gacctggccc ctgtcactta
     6181 ccgctcgagg cggcggagct tttctggaag ccaaaggctt tttgcagacc ggcgggcctg
     6241 atctggacgg ggcattgact gtggccctca acggacgccg gctcagtgag cttgatcccg
     6301 atcttcctcc ggttggtccg tatgctctca gggctcaggt gattaagaag ggtgaccggt
     6361 acgaagtgag tgattttcgc agccggtttg gaaacagcga tttttccgga attctggaaa
     6421 tgaatttcca gaaggccagg ccatatctca cggggatctt tacatcgaaa cacatcgagt
     6481 ggactgaact ttccgggccg gagaatgagg gtgagagtgc cattccgtcg gaggggatgc
     6541 gcgcgatcga tgttgatctg aacatggtga tcaaccacgt tcgaaatgga agactgaatg
     6601 tggccggtct gacctttacg gctggacttc aggcgggtcg gctgaaggtg gaatctgttc
     6661 aaggaacagt cctggatcag caatccgcat acggaaattt tcatggcggg ttccaattgg
     6721 ataccaccag gccgattccg acgatttccg gcaacgtgtc ccttgaccat gtccggtacg
     6781 aacatctctt ccccgaggcg ggattcgtag atcttgccga gaatgtcgtg aacctgaatg
     6841 cggaattttc cggtgcgggt cacacgattt ccgacatgct gaaccattct acggttcggg
     6901 tagatggaga aaagctatcg gtaaggtttc atcgtgaggc cgatcatccc gcctccgtgc
     6961 aggtgatggc aaacttgaaa gtggaaactg tggatggtgg gccgttgcgg ctttatgtcg
     7021 agggagtctt tgacaccaca tcatttcgac ttcgatcgtc aaccggttcg atgatgggcc
     7081 taatgaaaga caggggattg tggcccatga atgtccggct ggatctacca gaggcggtgg
     7141 ttgaagtgag cggacacctt acccttcctc atccggccca agaatttacg ttgcaggttc
     7201 tcgtaaaagg aaataatctc agagatctga actttctgac agcagccagt ttgcccgatg
     7261 ccggcccatt ggacataacg ggtctggtta cgaggtctcc tgtgggatac cacgtcaccg
     7321 atttaaaagg tgttctagcc ggaagcgatg tgcaaggcca tgtgatggtc ttaacgaaag
     7381 gaattcggcc acgagtgaga gggaaactca ccgcaaacaa tcttgtcctg ggtgcggtga
     7441 aacaaccttt agatgattcc tcagcacaac aaaagcggtc gacgctgaaa gcctttggtg
     7501 acgaggtccg gggcatcggg tcatcggctg ttaatgtcgt acgcgatacc atcggtatgc
     7561 ggaaagactc tggggctctc ccactcaagt cgattccgga ttgggttttt ccaattgagg
     7621 atctcagggc cattgatctc atgctggatg ccgaaatcaa acacattcga agggcagaag
     7681 aggatctggg acatgcctca tttcagatcg cccttgaaga aggcttgctc acactgcatc
     7741 ccctgaccgg aaatctttgg ggaggagact ttgaggggaa atttgtcctc gatggcacaa
     7801 aatacgttcc gacccttgat gtggaattgc acatccatgg cctgaattat gggcgcgtcg
     7861 ccagatattt cggtggctcc gatctggtga aggggaaatc ccaatccatc ctactcgccc
     7921 ttcagggtcg aggagatacg ttgcatgaag tgttggaaca agccagtgga cgatttgaac
     7981 tggttgatgg tcctcttgaa ttggccacaa aatatatcga tttgtgggcg gctgatttga
     8041 tcacgacggc attaactacc gcctggaaaa gtgaatcggt caccaaattg aattgtacgg
     8101 tcgggtattt tgatattgaa gagggcgtgg tgaaaagtga tgatatcctc atcgactccc
     8161 atcgactgac tatcgcgggc atcggaaaat tgaatttggc cgacgaaacg ttggacatag
     8221 tcctgactcc caggcccaaa gaccccagtt tattcagtct ggctcacacg gtccgcatca
     8281 ctggtccgct ttcgaatccg aatgtgtcca gcgacaaact ccggattgcg gaaagcgggg
     8341 gttgggggct actgggcttg gtcactccaa tgggatgggt tattgcgatt ccacagatag
     8401 cgggaaccac tgtgggcacg atgaatcaaa atccctgtgt tgaggccctg aagggtcgac
     8461 cgcaaaccgc tcaggccctt gatgagatca aaggcggttt gtggggaaag ataaaaaggg
     8521 cgtttagtaa tcttgccgga ttttcggaaa ctccttcagc taatccacag taaaggaatc
     8581 tatcggtgtg gtcttccacg gattgtgcgg gcataaatac gatgataacc catccggctg
     8641 ggaaaaaaat gtgagccgca tcgaataacc ataatgtgga gaggagagtg tttttggtaa
     8701 cctccaatgt atggaggaat attggaaaat aaccatcatg aaattcgtga aggctgagga
     8761 atagcggtct tcaacgaatg ttaatagact atatagcgct aataagtatt acgcattcac
     8821 aggtcttttt taaccaatcc ctcggggtaa tcctcagatg tgaattgagg ctggaaggga
     8881 atgccgccaa tgtttggatg agcgtgttca aggcagaatg gtcgtttaag aaaaaccaca
     8941 tggacaactg cagcccagcg aatcctatag tcagacccga ctagagagat cggggctttc
     9001 acaaaaggcg acaatccatg cgtttcccga ttcagacatt gcaagttctc aaagttgcag
     9061 taatgattgc aattttaatc agttttccaa agacaggtct tggaataacc aaggccatca
     9121 caacccctcc tcccgtgagt ggccatgaac tttcaattga atccatcacc ccggctgatg
     9181 tgttcgcacg tgtgcaatta atacggaatg aactggacga cctccgattt gaaatgggga
     9241 gacctaagag tcacgaaatt ggcctcctgg ttgaaaatgc gactccgcac gaggtgttct
     9301 ttcaagccaa aacccttaat caaaaagtgg ccaggttggt cacggaactc accgaggaat
     9361 cggaggtcgg tgggtcagaa gaggatccgg tcgatatacg tccctatcat gtctggaaaa
     9421 tgatcgattc ctctctgcac cgaatcctct ctcttaactc tctcttaagc ggcaactcgg
     9481 actcagcctg acgaacagtg aagcctaggt cgatgctgaa acgaccccca atcaagtctt
     9541 ttttatcatt ggattggcga atacccaact caatctttta ctgatgcatc agtttttccc
     9601 gaaagatgca gcagagttgg tcggcctggg catcgaatac accacacaac ttttgcgtca
     9661 atttcctacc gctcccgtcc cggctcctct cccttcgctt gaacgagggc gtaagtcatg
     9721 gaatagctac gaccatctta ttgattgcta tatggtgtta ggaaagatcg ctcgggcctc
     9781 caatattaaa atgttaacgt tggcccctca gaatctagat cgtcccgata ttcagtcgaa
     9841 tgatgtctac gatttagcaa ccctggtcat ctctgaactc gcgtatctcc atgcccacct
     9901 tcctcatatt cctccaccat ccccggtcag acttcgtggt cctgtcttgc cttcccatgt
     9961 gtatcaacag gtaggtggac tcctggctca attgaaggta ttacaaatgc aggttcaatc
    10021 tcatcccgaa tggctcaaat gattgttcgt gtggatgaac gcccggaggc aagatcacgt
    10081 attactgcat gactcaccaa cttgcggtgg aacatccaac ggttggcaaa tcctctgtgg
    10141 ttcccctgat tcgaataggc aacgtttctt gtggttgcct gcgcaagatt tcatgctcgt
    10201 tgcagccctt ctccgtcttg ggaaagtatc gctaccttgg attttgagga gtagcgctca
    10261 attttcacca ccctggtggc ggcatgcctg atagcagttc gttgagaatg attgatctaa
    10321 tttcagagtg aagagacacg cctcatttat aatgttgata atgaaaaaaa ctgaggcgac
    10381 caatctaata ggtgttattg tgcggagcca acgggtatcg atcacagcga aatgaaaacg
    10441 acaggaggcg ttttttcttc ctgggaatgt tcgcaatagg tcacgccaat gcatccggcg
    10501 aaagcaaacg gtgtcttggg taacttcacc gacattactg gaggttatgc aatctcggat
    10561 atagacaata cctgggaagg atcgttctaa ccatcgattg aatatgtgtg aacaataggg
    10621 cggccacatt caagaagcgt attttgagcg gagttccggt ttttcctaga tgtttttttc
    10681 cgatggtgga gtgtcgggag ccgactcaac ttcctgcttt tctttctttt tgggtggttt
    10741 ggcgggcgga gtgtaccagt gatctttcgg tacgattttg ggttgtgccg gatccgtaat
    10801 ataggccgga gtcatgattt gaacaccatg ttcgttgaac acatcgagaa tattctggct
    10861 gagaccggta tacagttcca acatgtaata cggccggtcg cagtagacgt tgagttcgta
    10921 ggtgacgcag aaatcgccca aggcgagttg caggacaaag ggcgggggat ccttcaacaa
    10981 accgggtgtc cggtccgcgg cctcaatcag catcgcctcg acctgcctcc acggggtctc
    11041 gtatccgatt cccaccgtga tatgaagaat gaggccattc tctctggcgg cggcgctata
    11101 gttggtcacg tggctgttca gaatttccga gttgggcacg atgatttctt cgtttttcaa
    11161 cgaccgaagc cgggttgtca gaattctcct ttctatcaca tcgccgagaa tttcattgac
    11221 tttgatacgg tccccttcct ggaacgcccg ccggtaaacc agggtatacc cggccaggat
    11281 attaccgata atcgaggagg acccgagcga caggagcacc ccgaggaaaa tcgtgatccc
    11341 tttaaaggcc tcggaggtgg atccgggtat gtagggatat cccaccacaa tcccgaaggc
    11401 gatcaccagt gtccggaaaa tccggtaggt gggccaggcc cattcccgat cgaaccccgg
    11461 ccatgtcacc tttccgatgg ccagattggc aaaaattaat ttcccaaact tcaggaggta
    11521 gcgcagcaag aagaccagaa taacgagaaa aaccaaactg ggaaatgagg aaagaaatcc
    11581 ctttacaatc acccgaatcg ggtcgaggat gatatcgaac aactgcttgc caatataggc
    11641 ggtccaagga aactgacgga acacaaaatt gagatacgca tacagcagca aaaagagcag
    11701 gaatgtcctg attccgttca ggcttccacg gagcccggcc cacatctgat gggcctgaag
    11761 gataatgact ttttgaacgg ttacatcctt gacccgttct ttgtatcgtc gctcgattac
    11821 cgcatccagc ttgtcgaaaa cccatcgggt tccccaccag gcagacgcaa gaatagctgt
    11881 ggccagcaag gaaaacgcaa tgtgaatcaa gatgttgatg ggtttgcgtg tctccctgtg
    11941 ggctaccacg gcttcctcga tccgtttaag gtagatccga gccaggattc cacgctccac
    12001 tccgccttcc agccgaccgt cggcatcggt caccccggct aatggttgat cgcccgcgaa
    12061 aatcatggtg accccttttt cctcaaccag gcgtagggat tggggtgaca gactcagatc
    12121 ggagccaaac tcatcaatgc gctcaataat ccttcgggcg cgttcctctg cgggtaaggc
    12181 cgacacgccg cgtacctgaa agagcgtttc cccatcaagt atgacgggcg cggtgggaac
    12241 cagaatcggt ggaaggac
//
LOCUS       DATIAL010000573         6304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_746600, whole genome shotgun sequence.
ACCESSION   DATIAL010000573 DATIAL010000000
VERSION     DATIAL010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746600"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /gene="ahcY"
                     /locus_tag="VLA60_17435"
     CDS             complement(<1..1374)
                     /gene="ahcY"
                     /locus_tag="VLA60_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HSF11198.1"
                     /translation="MSTATMTKAAAADYKVADIRLADWGRKELCIAETEMPALMALRK
                     KYHDSQPLRGAKILGCIHMTIQTGVLIETLIELGAEVRWSSCNIFSTQDHAAAAIAAK
                     GIPVFAWKGETEEEYEWCLEQTILKDGQPWDANMVLDDGGDLTMMLHKKYPAMLTRIH
                     GITEETTTGVHRLQEMLKNGTLKVPAINVNDSITKSKNDNKYGCRHSLNDAIKRATDH
                     LLSGKKALVIGYGDVGKGSAQSLRQEGMIVKISEIDPICAMQACMDGYEVVSPYRGGI
                     NTGKVEDINSDLLQKTDIVVTATGNLNVCDSAILQSLKCGAVVCNIGHFDNEIDTAFM
                     RKNWEWEEVKPQVHTVYRDKASNDHLILLSEGRLVNLGNGTGHPSRVMDGSFANQVLA
                     QIYLYERKFADQKDGKITVQVLPKHLDEEVAAHMVRGFGGVITKLTQTQADYIHVPVN
                     GPFKEDSY"
     gene            complement(1475..2626)
                     /gene="metK"
                     /locus_tag="VLA60_17440"
     CDS             complement(1475..2626)
                     /gene="metK"
                     /locus_tag="VLA60_17440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HSF11199.1"
                     /translation="MRQTNFLFTSESVTEGHPDKIADQISDGILDAIIAKDKNCRVAC
                     ETILTTGIAFVAGEISTRAYVEIPEIIRETIREVGYTDATWGFDYSTCSVLTAIHNQS
                     GDIAMGVDSGGAGDQGLMFGYASNETPELMPMPIILAHRLTRRLAEVRKKNILPWVRP
                     DGKSQVTVEYKNGKPIRIDTIVLSTQHSDAVTSKKIEKDVMEKVIIPVMPKKFFNQKN
                     IKFHINPTGRFVTGGPMGDTGLTGRKIIVDTYGGVGSHGGGAFSGKDPSKVDRSASYM
                     ARYIAKNVVAAQLAEKCEVQLAYAIGVAEPVSVLVDTKGTEKASVEQIEKLIIKHFPL
                     TPRGIIEHLKLRRPIYKRTAAYGHFGRNEPGFTWEKTDLANLLRKGAGL"
     gene            3389..5488
                     /locus_tag="VLA60_17445"
     CDS             3389..5488
                     /locus_tag="VLA60_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HSF11200.1"
                     /translation="MYKTIYVPVDNSDHSNMAVELGVQFAKIFGSKIVGSHVYAAKMH
                     DKRFKQMEAGLPEEYHDEKELDRQRQIHDSLITRGLQIITDSYLDFVDQKCAEANIPL
                     ERRSLEGRNWKAIAEDITKNGYDLTIMGALGVGAVKDSVIGSNTERVIRRIRNSDMFI
                     VKDTKPMNGGKIVVAVDGSHYSFGGLKTALALGKALNKPVEAISAFDPYFHYAAFHSI
                     SGVLNEEAGKVFRFKEQEKLHEEVIDSGLAKIYQSHLDVSRDVAQEEGEDIKTTLLDG
                     KAFEKIIQYVRKEQPWLLIVGRIGVHSDDDMDIGSNAENLLRSAPCNILISNKKFVPP
                     IDTQAEYTIAWTEEALRRMEKIPVFARGVAKTAIHRYAIEKGHTIISNSVIDDAVGDI
                     LPKGAMDAMKTLGGTLDAAGVDRNTMQAGNEVAQDLMGSTLSGMMSQVVEEIPAETSE
                     PMSAGNKSYLARMSRDYYVCGGCGYIGKGDHPVMCPVCGLDGAQFKQVDKSIFDAAAK
                     AEGGLETQVAYDDIPMQWTKDAREAIRAVPAGFQRRRAKAKIEKTARKLGMTTITLEY
                     SGAMIQEAASEDYTPIFANKTAGETATMVSESNKESTGDTNGADPFIWEPDALQRLER
                     APAGFMRDCTKALIIKHAEKIGTTTITLDVANQGIEQAKTTMEEAMKTGNVKDIVAKL
                     TGAGAPSEAQSGAGHNG"
     gene            5481..>6304
                     /locus_tag="VLA60_17450"
     CDS             5481..>6304
                     /locus_tag="VLA60_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HSF11201.1"
                     /translation="MGKSLPVISSFFNTLSSLGASAVTKFENFRPESGGPGDGRTVDD
                     FKPYLVALNLTKRCNLKCEHCYLDATTKMGGGHDELTTEECFRLIDQIAQVNAGSLLV
                     ITGGEPLVRPDILEIARHAVEQRFMVVFGTNGMLIDDKMAKAMVDIGVMGVGISIDSL
                     DSATHNAFRGLPGAWEGAMAGIEACKRNGLQFQVHFSAQPMNYKELPAVIDWSHDLGA
                     KVLNVFFMVCTGRGEELTDITPSQYEEVLSYLVESQDKYQDMLVRARCAPHFKRLA"
BASE COUNT         1630 a         1374 c         1575 g         1725 t
ORIGIN      
        1 gtagctgtct tctttgaacg gtccgttgac tggtacgtga atatagtcag cctgtgtttg
       61 agtgagttta gtgattaccc ctccaaaacc tctaaccata tgggctgcca cttcttcatc
      121 gagatgtttc ggtaaaacct gcactgtaat ttttccatct ttctggtcgg cgaatttccg
      181 ttcgtagagg taaatttgag cgaggacttg attggcgaat gagccatcca tgactcggga
      241 tggatgcccg gtcccatttc ccaaattcac caagcgccct tcggacagca agatgaggtg
      301 atcattgctg gctttgtccc gatagactgt atgcacctga ggctttactt cttcccattc
      361 ccagttcttt cgcataaagg cagtatcgat ttcattgtcg aagtgtccga tattgcaaac
      421 gaccgcccca catttcaagg attgcaggat ggctgaatcg cagacattca agttgccggt
      481 agctgtgacg actatatctg tcttctggag aaggtcggag ttgatgtctt cgaccttgcc
      541 tgtattgatc ccacctctat agggagagac cacttcatac ccatccatgc aggcttgcat
      601 ggcacagatg ggatcaatct ctgaaatttt gacaatcatg ccttcctgtc gaagtgattg
      661 agccgagccc tttccgacgt cgccataccc aatcacgagg gcttttttcc ctgacaggag
      721 gtgatcagtc gcgcgtttga tcgcgtcatt caagctgtgt cggcaaccat acttgttatc
      781 attttttgac ttggtgattg aatcattcac attaatggct ggaactttta gcgtgccatt
      841 ttttaacatt tcctgtaggc gatgaacccc ggtggttgtt tcttcagtaa ttccatgaat
      901 acgggttaac atggccggat attttttgtg gagcatcatc gtcaagtccc caccgtcatc
      961 cagaaccata ttggcatccc acggttggcc atcttttaaa atggtttgtt ccaggcacca
     1021 ctcatattct tcttctgttt cacccttcca ggcaaataca ggaattccct ttgcggcgat
     1081 ggcagcggcg gcatgatcct gtgttgaaaa gatattacag gaagaccagc gaacttcagc
     1141 tcccagttcg atgagtgttt caattaacac tccggtctga atagtcatgt gaatgcagcc
     1201 aagaattttt gcccctcgta atggttggga gtcatgatat ttctttcgaa gagccatcaa
     1261 cgccggcatt tctgtttcgg caatgcataa ttcttttcgt ccccaatcgg ccagacggat
     1321 atcggcaact ttatagtcag ctgcggcggc tttagtcata gttgcagtgc tcatagaaat
     1381 ttcttcccct tctcaggatg agtgattgaa gatgctggac tcaagaaggg ggccttgaga
     1441 tcccccttat tcaaaaggcc gaggcgctta aaaattaaag gcctgcacct tttcggagta
     1501 aatttgctaa gtcggttttt tcccaggtga atcctggttc gtttcgccca aaatgtccgt
     1561 atgctgcagt tcttttgtaa atcgggcggc gcaacttgag atgttcgatg atgcctcttg
     1621 gagtcaacgg aaagtgtttg atgatgagct tttcgatctg ctcaacagac gctttttctg
     1681 tgccctttgt atcaaccaaa acagagactg gttctgcaac tccgatggca taagccaatt
     1741 gcacttcaca tttttctgcc aattgggcgg caacgacatt tttagcaata tatcgggcca
     1801 tgtatgaggc tgatcggtct accttgctgg ggtcttttcc ggaaaaagcc cctcctccgt
     1861 gactgccaac cccgccataa gtgtctacga tgatttttct tccagtcagt ccggtgtctc
     1921 ccatggggcc accggtgaca aatcgtccgg ttggattaat gtgaaatttg atattttttt
     1981 gattgaaaaa tttcttaggc atgacgggaa taatgacttt ttccatcaca tctttttcga
     2041 ttttcttcga ggtgaccgca tcgctgtgtt gggtggaaag gacgatggtg tcaatgcgaa
     2101 tgggtttccc gtttttatat tcgactgtca cctgtgactt tccgtcaggc cgaacccagg
     2161 ggagaatatt ctttttccga acttctgcca gtcttctggt taaacggtgc gccaggataa
     2221 tgggcattgg catgagttca ggggtttcat tcgaggcata cccgaacatt aacccttgat
     2281 cgccagcgcc acctgaatcc acacccatgg caatgtcgcc tgattggtta tgaatagcgg
     2341 ttaacaccga gcaggtactg taatcgaatc cccatgtcgc atcggtatac cccacctcac
     2401 gaatcgtctc acgaataatc tcaggaattt ctacatacgc cctggtggaa atttctccgg
     2461 ccacaaaggc gattcctgtg gtcagaatgg tttcacaggc caccctgcag ttcttgtctt
     2521 ttgcaatgat tgcgtcaagt ataccgtcgg aaatctgatc ggcaatctta tccggatgcc
     2581 cttccgtaac cgattccgag gtaaagagaa aattggtttg tctcatcaag agttccccct
     2641 aaccaattgc ttgagcccaa atgccctgaa tggcatggtc tcagttggtg aataaagcaa
     2701 ggcacaaaac acttaattta gcgcaattaa acctctgtcc gcaatttttg gtttggctat
     2761 aactgaaaaa cttgaggctt tcctggtttc gttctttttt tttcggctaa aggttagtaa
     2821 aacataaaat tcccataacg caaagttctc ctaaagaaaa ttaaaaaagt gagacggact
     2881 aatgaatggc ttcctcattt cgattgattt gcctggagcc cagcctgtta gtaactctac
     2941 agggaggatt aggttgcagc attagttgcc atctgtggca attcgaagct cgtttttcgg
     3001 atgattaagt cccgcgtcgg ttcttaaaga aggatatgaa tagtggttcg tttagtgaaa
     3061 gcttggtaag cgccaactgc ctgtccatgt gtaacctaag ggctacgcac cataattggt
     3121 gagtgtttta acgtgctttc catgaatttg tagtggacgg ggtaaatgct aatatgagcc
     3181 agtcaatgca tttgataaat ataggatttt ttatatttca ctcatgtcaa gctggaaatg
     3241 gtcgttgggt tgcgtaccta gaattcccag tggtaagatc cgctcttatt tcatgtgaaa
     3301 aaacatctgt ttataaaaat tcccggcgat tgagacgagg gcctggtacc gctttttttt
     3361 tggcggaaat ctgtaaggag gcttgttgat gtacaaaaca atatatgtcc cggttgataa
     3421 ttcagaccat tcgaatatgg ctgtggaact gggagtgcag ttcgcgaaaa ttttcggatc
     3481 gaaaattgtg ggaagccatg tatatgcggc caagatgcat gataagcggt ttaaacaaat
     3541 ggaggcaggt cttcctgagg aatatcacga cgaaaaagag ttagatcgcc agcgccaaat
     3601 ccatgattcc cttatcacca gaggattgca aataattaca gattcgtatt tggattttgt
     3661 cgaccagaaa tgcgcggaag cgaacattcc tctggagcga cgctcacttg aggggcgaaa
     3721 ctggaaagca atcgcggaag atatcacaaa aaatgggtat gacttgacca ttatgggagc
     3781 cttgggagtt ggtgcggtga aggactctgt gatcggtagc aacacggaac gtgtgatccg
     3841 tcggattcgt aattcggaca tgttcattgt gaaagatacc aaacccatga atggtgggaa
     3901 aattgtagta gctgtggatg ggagccatta ctcctttggt ggactgaaga ccgcgctcgc
     3961 cctgggaaag gctctcaata aaccggtaga agcgatatca gcattcgatc cctattttca
     4021 ttatgcggcg tttcacagta tttccggtgt tttgaatgaa gaggctggga aggtgtttcg
     4081 gtttaaagag caggaaaagc ttcacgagga agtcatcgac agcggacttg cgaaaattta
     4141 ccagtctcat ctggatgtct ctcgcgatgt cgcacaagag gagggcgaag atattaagac
     4201 cactctgctt gacggcaaag cgtttgagaa aatcattcag tacgttcgaa aagagcagcc
     4261 atggctgctt atcgttggac ggattggggt gcatagcgat gatgatatgg atattggcag
     4321 caatgccgaa aatcttttgc gttcagctcc ttgtaatata ttaatatcca ataagaagtt
     4381 tgttcctccg attgacaccc aggctgaata cacgattgcc tggacggaag aagccttaag
     4441 acgaatggag aagatcccgg tctttgcgcg gggagtggca aagactgcga tacatcgata
     4501 tgccatcgaa aagggtcata ccatcatcag taattcagtt attgatgatg cggttggtga
     4561 tattttaccc aagggtgcga tggacgctat gaaaacgcta ggtggtaccc tcgatgcggc
     4621 cggagtcgat cggaatacta tgcaggcagg gaatgaagtt gcgcaggacc taatgggctc
     4681 aacattgagc ggcatgatgt cccaggtggt ggaggaaata ccggcggaga cttctgagcc
     4741 aatgagcgca ggaaataaat cgtatctggc tcgaatgtcg agagactatt acgtctgtgg
     4801 tggctgtggg tatattggga aaggcgacca tccggtgatg tgtcctgttt gtggtttgga
     4861 tggagcgcaa tttaaacaag tcgataaatc tatttttgat gcggctgcca aagcggaagg
     4921 tggcttggaa acccaagtgg catatgacga tattcctatg cagtggacga aagatgctcg
     4981 tgaagcaatt cgagccgttc ccgctggttt ccaacgccga cgggcaaaag cgaaaatcga
     5041 aaaaactgcc aggaaacttg gaatgaccac cattaccttg gaatattcag gcgcgatgat
     5101 tcaggaagcg gcaagcgaag actacacacc aattttcgcc aataaaacgg ctggggagac
     5161 ggcaactatg gtctctgaat ctaataagga aagtactggc gacactaatg gagccgaccc
     5221 gtttatatgg gagccggatg ccctccaacg attagaaagg gctcctgctg gatttatgcg
     5281 ggattgtact aaagcgttaa tcattaagca tgctgaaaaa attgggacca caactataac
     5341 tctggatgtg gcgaaccaag gcattgagca agctaaaacc acaatggaag aagccatgaa
     5401 aactggaaac gtcaaggaca tcgtggccaa gttaaccggc gcgggggctc cctctgaggc
     5461 tcaatccgga gccggccaca atgggtaagt cactccctgt catcagttca ttcttcaata
     5521 cgctcagctc gttaggggcc agtgcagtaa caaagtttga aaattttcgt ccagagtcag
     5581 gagggcctgg agacggccga actgtggatg attttaagcc ctacctggtg gctctcaatt
     5641 taaccaagcg atgcaatctc aagtgcgaac attgttacct ggatgccacg acaaaaatgg
     5701 gcggtgggca tgatgaattg acgacagaag aatgtttccg tcttattgat caaattgccc
     5761 aagtcaatgc cggaagcctt cttgtgatta ccggaggcga gccgttggtt cgtcccgata
     5821 ttcttgaaat cgcgcgtcat gcagttgagc aacgatttat ggtcgtattc ggcacgaatg
     5881 gaatgctgat tgacgacaag atggcgaaag ccatggtaga cattggtgta atgggcgtag
     5941 gaattagtat tgattccctg gattcagcaa cacacaatgc gtttagaggt ttacctggtg
     6001 catgggaagg ggctatggct ggtattgaag cctgcaaacg aaatggactt caattccaag
     6061 tacatttcag tgcacagccc atgaactaca aggaattgcc agccgtcatt gactggtccc
     6121 acgatttggg tgccaaagtc ttaaatgtct ttttcatggt gtgcacagga cgaggtgaag
     6181 agctcactga catcactcct tcacaatatg aagaggtttt gagctatttg gtggagagtc
     6241 aagataaata ccaggacatg ttggttcggg cgcgttgtgc accacatttc aaacgcctgg
     6301 ccta
//
LOCUS       DATIAL010000574         4819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1284326, whole genome shotgun sequence.
ACCESSION   DATIAL010000574 DATIAL010000000
VERSION     DATIAL010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284326"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            476..1525
                     /locus_tag="VLA60_17455"
     CDS             476..1525
                     /locus_tag="VLA60_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HSF11202.1"
                     /translation="MNDDKQTTLPAEKSSPPPLADHTKPDSLRRQSVPGKPSSKARTP
                     SLNSPESSHAETVSMLKKQYQLAKRQKRWIKRAGVMGIVLILLGGLGTWLAGENKAFK
                     MAILKVVAPVVSIHLDPEMVSIPAGTFQKGDSREENSSSEQPLREVHIKKFALGRFEV
                     TFEEYDRFAISTGRPLPGDEGWGRERHPVINVSWKDAVDYATWLSQETGYRYRLPTES
                     EWEYAARNMGKGEIWAGTSEQEQLATYAWFNTNSNGRTQPVGTQQPNALGLHDLSGNV
                     WEWVQDCWHDDYQHAPTNGSAWLEANDGSCGTRVRKGGGWTNAPMSLRSSFRTGYSAD
                     SRSIQIGFRLAQDIE"
     gene            complement(1620..2894)
                     /locus_tag="VLA60_17460"
     CDS             complement(1620..2894)
                     /locus_tag="VLA60_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HSF11203.1"
                     /translation="MTYWLMQNLVCRRIQRLTCFLVLAMLVISQSVTQAEELKNVIPD
                     LYGGNGFQTDPNFAGNRSNDFGNAVFNSFNELTNGIGAQTGQFAVNSTVAAYRFDVER
                     GVPVEVTKSLGPLFAERADTLGKGRFDFQVVWSNANYKKFNGQKLSSLSADVSPTAGP
                     GTPPNAPGLDELLQIDLDVKVITNFVGFFATYGLTDNWDVNLLVPLINNKIKAKAKAT
                     MLDANGVPCTQGGNCSGIYVINGTAPGTGDPSKDSSSGDKTGIGDVVLRTKYNMLKNH
                     EILPDLGVRGDVSFPTGNEDNFMGVGEFKFQGLAVASKYYSSPVGMIGPHVNTGFVLV
                     NNKSELNLFTYVAGFDFAPIPVFAFSLDLLGRHELDDKDNSGKDIVDLAPGLKWAPLP
                     NTLVQAAVQLPLNKNEGLRPDAVWTLGLGGTF"
     gene            3666..>4819
                     /locus_tag="VLA60_17465"
     CDS             3666..>4819
                     /locus_tag="VLA60_17465"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSF11204.1"
                     /translation="MNGKVPAQYIAESQGKSWAVIIGIDKYLNLADLAYAVDDAKSVA
                     RMLGRQGFTVTSLYNTQATKKAILQELRQNLVTQVQKNDRVLIFFAGHIGETPGKGKQ
                     QPVGYMMPVDTEPGLLADTAISVDLLRELSESLPAKQVLFLIDICYGGIAGRSQRSFP
                     PMTSNYLKMIASEPARQLITAGGTGQQALAGPKWKHSVFTYYLLEGIGKGKGDLNGDG
                     IIPTSELFAFLDEHVQSAALTHNHAQRPEMWGLSADKGEFVFIPEKFSSNAQVAHTPD
                     RQGKASDPTPGTVETIVGLLKSFGNPLDVFNKVPSGKSEQADKQKTVEEEAAALEAER
                     EKFELQALQSLKEQRQRERDLQAQLAEAQRLAAEEERRRVAAEQARQEQEA"
BASE COUNT         1327 a         1211 c         1156 g         1125 t
ORIGIN      
        1 aggatcgcaa aattaaaagg tatcgggaag accccaccga ctgttctgtt atagattcaa
       61 tcgttccctc ccacccttcc aaccgtgttc atcgcgacct ctgcgtttaa aaacattctc
      121 atacgtcagg gggcatcgct cacgttctaa gctttgaagg tcctgataca agtcctcttt
      181 tcttccaaaa tgctacgggt tcaaccgttt gtccaaattc acgcccgcaa gtccttgggt
      241 aatccttatt gaatttttac attaagaaaa actgcttttg ttcaggaagt tatctgttaa
      301 aatagcttga catgatacta ggttggtccc gcaagttaat gttagaggac tcattccgtc
      361 atgatgggaa aaaggccata tcctctaaca ggacgaatag ccacagaaga atgaggaata
      421 ttcccgatag gccaccgatc tcttatcgca tgtgcatgct tggatcgcgc acccaatgaa
      481 cgacgataag caaacaaccc tgcctgcgga aaaatcttcc cccccacccc tggctgatca
      541 cacaaagcct gacagcctca gacgacaatc ggttcctggc aagccttcgt ctaaggcccg
      601 aacacccagc ctgaattcgc cggaatcttc acatgcagaa accgtgtcca tgctgaaaaa
      661 acagtatcag cttgcaaagc ggcagaaacg atggataaaa cgagcaggag tcatgggaat
      721 cgttcttatt ttattgggtg ggttgggcac atggttagca ggggagaata aagcttttaa
      781 aatggccatc ctgaaagtcg tcgctcccgt ggtgagcata cacctggacc ccgaaatggt
      841 ttcaattcct gccgggacgt ttcaaaaggg agatagccgg gaagagaatt cgtcaagcga
      901 acagccgcta cgagaggtcc atatcaaaaa atttgccctg gggcgattcg aggttacctt
      961 cgaagaatat gaccggttcg ccatatcgac gggtcggccg cttccgggag atgagggatg
     1021 gggacgtgaa aggcatccgg tcatcaatgt ctcatggaaa gacgccgtgg actatgcaac
     1081 gtggttatcg caggaaaccg gttatcgcta tcgacttccc acagagtctg agtgggaata
     1141 tgcagccaga aatatgggaa aaggcgaaat ttgggcggga acctcagaac aggaacagct
     1201 tgcgacatat gcctggttca acacaaacag caatggaaga actcagccag ttggcaccca
     1261 acaacccaac gccctcggcc ttcatgatct gagcggaaat gtatgggagt gggtccagga
     1321 ttgctggcac gacgactatc agcatgcccc cacaaatggg tcggcctggt tagaggcgaa
     1381 tgacgggagt tgcggaacac gtgtgaggaa aggtggtggc tggactaacg cgccaatgtc
     1441 tttgcgttct tcgtttcgta ctgggtacag cgccgactcg cggagcatcc agattggttt
     1501 tcgcctcgca caggatatcg aatagcatag gctatcttcc catttttgta ttctcttgcc
     1561 accgactccc tgccaggagc atcactcaat taggatcatc caaggagtga accggcgacc
     1621 tagaaagttc ctccaaggcc taacgtccag accgcatcag gacggagtcc ttcattttta
     1681 ttgaggggaa gctggaccgc tgcctggacg agcgtgttgg gaagaggagc ccattttagg
     1741 ccgggagcca aatcgacgat gtcctttcca ctgttatcct tatcatccaa ttcatgacgg
     1801 cccaggagat caagtgagaa ggcaaagacc ggaataggcg caaaatcaaa ccctgcgaca
     1861 taggtaaaaa gattcagttc ggatttgttg ttgacgagca caaagcccgt attcacatgg
     1921 ggaccaatca tgccaaccgg agatgaataa tatttagaag ccacagccaa accctggaat
     1981 ttgaattctc cgactcccat gaagttatcc tcgtttcccg tgggaaaaga aacatccccc
     2041 cggacgccca aatcaggcaa gatttcatga ttcttcagca tgttatattt ggttcgaagg
     2101 acaacatccc cgattccggt tttatccccc gaagaactgt cttttgaagg gtctcccgtg
     2161 ccgggtgccg ttccattgat cacgtatata ccactgcaat ttccaccttg agtacagggg
     2221 accccgtttg catcaagcat ggtcgccttt gcttttgctt tgatcttgtt attgataaga
     2281 ggaaccagaa gattgacgtc ccaattatcc gtgaggccat acgtagcaaa aaaccccaca
     2341 aaattggtaa ttactttgac gtccaggtca atttgtaata gctcatctaa ccctggggca
     2401 ttcggcggcg ttccaggccc ggcagtcgga gagacatccg cactgaggga agacagtttc
     2461 tgcccattaa attttttgta attggcgttt gaccagacga cctggaaatc aaatcgtcct
     2521 tttccaagcg tgtcggcccg ttccgcaaat aatggaccga ggcttttggt gacttcaaca
     2581 ggaactcccc gctcgacatc aaaccgatac gctgccacgg tagagtttac cgcaaattgg
     2641 cctgtttgag ccccaatgcc attggtcagt tcattaaagg aattaaacac cgcattgcca
     2701 aaatcattgg accgattgcc tgcaaaatta ggatctgttt gaaacccatt tcccccgtaa
     2761 agatcaggaa tgacattctt taattcttcg gcttgagtga cagattgact gatgacgagc
     2821 attgccaaca caaggaaaca ggtcaaccgc tgaattcgcc tacataccaa gttctgcatg
     2881 agccaataag tcattatttt tcctcatata tttggaataa acaaaaaagg tttcccaaaa
     2941 attgccacac aacgggtttg gcgcgatggt ccgagcgcaa ttatcgactt taactatggg
     3001 tttgggggat ggtacgctca acaccacagg tccaaaagaa tggtgttatt cacgcccttc
     3061 ttcagcttta gtggctatca gatggaatca tatccaaaaa acgcgatgct gatgacgtag
     3121 aaaaccaggc gatcctataa actctttctc atgaagtcaa tccatttttc ccagagggat
     3181 cgcctgcatc gaccccccac tcaggcatac ccccaaaatt agggaaatga ccgacaggct
     3241 tcaagaagga acgtccctta tttttcaagt cgtgtataag gccaacaatg gcatctccga
     3301 ggcaagaccc agtaaactgg tttcaaaaac ccacgccgtc cgagatcagc accatgcgta
     3361 ttacacgttc tttcaattgt tgccgtacat ataggaccta acgaaacgaa agaaaaaggt
     3421 caaagaggaa attctgatgc atcaagaaaa tgccccttgc ttagtttttt ccgagaattt
     3481 tcatgagtaa aatcgacaga agccacagga gcaagtgaac ggtgttcgat cgcattctcc
     3541 atcatcccat cctgagaaga gggtaccttt cctctcccag catcttgctg ctctcttggg
     3601 tgttatgcag tttgaccgcg tggctcttcc caatacagac acacgcagat accttcggga
     3661 ttactgtgaa cggaaaagtt cctgcccaat atatagccga gtcccaggga aaatcctggg
     3721 ccgtcatcat agggattgat aaatatctga acctcgccga cctggcctat gcggttgatg
     3781 atgcgaaatc ggtggcccgc atgctcgggc gtcaaggatt tacggtcaca tcactctaca
     3841 atacccaagc taccaaaaaa gcaatccttc aggagttgcg ccaaaatctt gttacgcaag
     3901 tacaaaaaaa tgatcgagtg ctcatctttt ttgcggggca tattggagaa acccccggca
     3961 agggaaagca acaacccgtt ggctatatga tgcccgtcga taccgaaccg ggccttcttg
     4021 ccgacaccgc aatcagtgtg gaccttcttc gtgaactttc cgaatcactt ccggcaaaac
     4081 aggttttatt tctcattgac atttgttatg gaggcatagc gggtcgaagt cagcgttcgt
     4141 ttccccccat gaccagtaat tatctgaaaa tgattgccag tgaacctgca cgccaattga
     4201 taaccgcagg aggaaccggg caacaagcct tagccggacc gaagtggaaa catagcgtgt
     4261 ttacctatta tcttctggaa ggcatcggaa agggaaaggg tgacctgaac ggcgatggga
     4321 tcattcccac atccgaactc ttcgcctttc tcgatgagca cgtgcaatcg gcggctctca
     4381 ctcataatca tgcgcaacga ccggaaatgt ggggattgag tgcggataag ggagaatttg
     4441 tctttattcc cgaaaaattt tcttccaacg ctcaagtcgc ccacaccccc gatcgtcaag
     4501 gaaaggcgag cgatcctacc cctggaacgg tcgagaccat tgtcggactc ttaaagagtt
     4561 ttggaaatcc cctggatgtc ttcaacaagg tgccatctgg aaaaagcgag caggctgata
     4621 aacaaaaaac ggtggaagaa gaggctgccg cgctagaggc agagcgtgaa aaatttgaat
     4681 tgcaggctct ccaatctttg aaggagcaac gacagcggga gagggatctt caagcgcaac
     4741 tggccgaagc ccagcgcctg gctgcggagg aagaacgccg gcgggtcgcc gcggaacagg
     4801 cccgtcagga acaggaagc
//
LOCUS       DATIAL010000575         2304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_962671, whole genome shotgun sequence.
ACCESSION   DATIAL010000575 DATIAL010000000
VERSION     DATIAL010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962671"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1102)
                     /gene="aroF"
                     /locus_tag="VLA60_17470"
     CDS             complement(89..1102)
                     /gene="aroF"
                     /locus_tag="VLA60_17470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HSF11205.1"
                     /translation="MIIVLKPEATEQDIEHLVDRLRSLGLKTQITKGEERTIVGVLGD
                     DRLLQGQPLSVFPGVESVTPILAPWKLVSREFQKNDSVIDVAGVKVGGNRITIMAGPC
                     AVEKLEITVGIAQEVAQAGATILRGGAYKPRTSPYSFQGLGQEGLEFLAEARRQTGLP
                     VVSEILDARDLGHFLEKADVIQIGARNMQNFELLKEVGAYDKPVLLKRGLSATIKEFL
                     LSAEYIMSRGNRHVMLCERGIRTFETQYRNTLDLSAVPMLKEMTHLPVIVDPSHATGN
                     WKLVAPMAKAAIAAGADGLLIEVHSNPECALCDGEESLKPSRFKDLMNDLKKVAQAVG
                     REL"
     gene            complement(1106..2209)
                     /gene="hisC"
                     /locus_tag="VLA60_17475"
     CDS             complement(1106..2209)
                     /gene="hisC"
                     /locus_tag="VLA60_17475"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HSF11206.1"
                     /translation="MALRVHPTITSLTPYSPGKPLDELERELGISRAIKLASNENPWG
                     PSPKALQVIERASASLHRYPDGGAHYLRQALAERLKMADDQILVGNGSDEIISLLVRA
                     FLSPGDEAVMADLTFVMYKLSVLGGHGVPVEVPLRNWKHDVEGMCDAVTDRTRLFFLC
                     NPNNPTGTMLTAKEIDGFLSRLPDHVIVVFDEAYYEYVRHPEFPDTLRYVREGRPMVV
                     LRTFSKIYGLAGLRVGYGCSTKEITGYLNRVRPPFNVNSLAQEAARAALSDDEHVAKS
                     RAMNEAEMAFLEERLTTLGLEPVPSQANFLYFNVGIDGKGVYEALLREGVIVRHIRGP
                     MIRVTIGQPTENRRFLESLVRVLPPVVSHKDAE"
BASE COUNT          586 a          659 c          511 g          548 t
ORIGIN      
        1 acctattata gtcacgtgac ggaagagggg gtctggaaag gaagtagtca tgacggaaag
       61 agggaagcta ggaatcacat acgaacgatc agagttcccg gccgactgcc tgtgctacct
      121 tttttaaatc gttcatgaga tctttaaacc ggctgggttt cagggattcc tctccatcgc
      181 acaaggcaca ttccggattg gagtgaactt caatcaacaa tccgtcagcc cccgccgcaa
      241 ttgcggcttt agccatagga gccacgagct tccaattgcc tgtcgcatga ctcgggtcca
      301 caatgaccgg caggtgggtc atttctttca acattggtac ggcactcaaa tcgagggtat
      361 tgcgatattg agtttcaaaa gtccgaatac cccgttcaca cagcattaca tgacgattgc
      421 cacgagacat aatatattct gcggataaaa gaaactcttt gatcgtggcc gacagccccc
      481 gcttcaacaa gaccggtttg tcatacgcac cgacttcttt taataattca aaattctgca
      541 tattcctggc gccaatttgg atcacatcag ctttttccaa aaaatgcccc aggtcacggg
      601 catccagaat ttcactgacc accggaaggc ctgtttgccg tctggcttca gctaaaaatt
      661 ccaatccttc ctggcccaac ccctgaaacg aatagggcga tgtgcggggt ttatacgctc
      721 cgccacgcaa aatagtggcg ccggcctgcg ccacctcctg agcgatgccc accgtaattt
      781 ccaatttttc tacggcacag ggaccggcca taatcgtaat ccgattgcct ccgactttca
      841 cgccggccac gtcgatcacg gaatcatttt tttgaaattc tcggctcact aacttccagg
      901 gagccagaat tggagttaca ctttccacac cgggaaacac actcaggggt tgcccttgta
      961 acaaccggtc atcgccgagg actcccacaa tggtccgttc ttcccctttt gtaatttgcg
     1021 ttttcaaccc taacgagcgg agacggtcaa ccagatgttc aatatcctgc tcagtcgcct
     1081 cgggttttaa cacaataatc atgacctatt cagcatcctt atgtgaaacc acaggaggaa
     1141 gaacccggac aagggattcg agaaatcggc gattttcggt cggctgtcca atggtgactc
     1201 gaatcatcgg accacggata tgccgaacaa tcaccccttc ccgaagcaag gcttcatata
     1261 cacctttacc gtcaatgcct acattaaaat acaaaaaatt tgcctggctg ggcacgggct
     1321 ctaatcccag ggttgtcagt ctttcttcca gaaacgccat ttcggcttca ttcatcgctc
     1381 gacttttggc cacatgctca tcatcggaaa gagccgctcg cgcggcttct tgagccaaag
     1441 aattcacgtt aaagggtgga cggacccgat tcagataccc cgtgatttct ttggtcgaac
     1501 acccataacc cacgcgcaat cccgccaatc cataaatttt cgagaacgtc cgcagcacca
     1561 ccatcgggcg cccctcgcga acataccgca aggtatcggg aaactccgga tgccgcacat
     1621 attcataata agcctcatca aacaccacga tcacgtgatc gggaagccgg gagagaaacc
     1681 catcaatttc ctttgcggtc aacattgtcc ccgtggggtt attcggattg cagagaaaaa
     1741 agagtcgcgt tcggtctgtg accgcgtcac acatcccctc cacatcatgt ttccaatttc
     1801 tcaagggcac ttccaccgga acaccatgcc ctcccaacac cgataattta tacatgacaa
     1861 aagtaagatc ggccatcacc gcctcgtctc ccggagagag aaaggcgcgg acaagcaggc
     1921 taatgatttc atccgagcca tttcccacca gaatctgatc atctgccatc tttaatcgct
     1981 ccgccaatgc ttgacgaagg taatgcgctc cgccatctgg atatcggtga agtgacgctg
     2041 acgcgcgctc gatcacttga agcgccttcg gtgacgggcc ccaaggattt tcattggaag
     2101 cgagtttaat cgcccggcta atacccagtt cccgttccaa ctcgtccaac ggctttccgg
     2161 gcgaatacgg tgttaatgag gtgatggtgg ggtgaacgcg caaagccatt agtgcatctc
     2221 cttccccgtg tacaaactca ggtcaaccaa gaaaatcata aatctccaat tttttccggc
     2281 tggggctttg tcccgctctt tcta
//
LOCUS       DATIAL010000576         5674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_209956, whole genome shotgun sequence.
ACCESSION   DATIAL010000576 DATIAL010000000
VERSION     DATIAL010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209956"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VLA60_17480"
     CDS             <1..216
                     /locus_tag="VLA60_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11207.1"
                     /translation="MPKCQGCIAKYNCAGDCYARSASSTGDPASAGYTERCHITRELL
                     KDNLLLALIFKMAGVNAGGSVQHTCPM"
     gene            complement(428..1054)
                     /locus_tag="VLA60_17485"
     CDS             complement(428..1054)
                     /locus_tag="VLA60_17485"
                     /inference="COORDINATES: protein motif:HMM:NF039960.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11208.1"
                     /translation="MISTRVISCLLAMAIALLAGCASMPPRHQENLCTIFDQYPEWYD
                     AAKESQEKWGTPPHILMAFVKQESAFRHDATPPWDWFLFIPLGPQSSAEGYAQAKDET
                     WEEYEDEAGGVFSSRSTMEDALDFIGWYNNKSSRELGISKWDPKRLYLAYHEGRSGYR
                     RGSYKRKPKVLRIANRVDWQARQYGAQLRQCEHRFRCRHWYQFWPFCS"
     gene            1747..2292
                     /gene="hpt"
                     /locus_tag="VLA60_17490"
     CDS             1747..2292
                     /gene="hpt"
                     /locus_tag="VLA60_17490"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HSF11209.1"
                     /translation="MLPHSVLIDEKTIETRVGELAKLIATDLPTRQPILLGLLTGSFV
                     FLADLVRHLSHHGIEPQVEFLKVSHYGDTSEASGSVSFLREKLPHITDEVVVVVDDIF
                     DSGFSLHAVHEHLKQQQPAWVRFCTLLDKPSRHQVALRVDYVGFEIPDVWVIGYGLDL
                     GGEGRALPYLAAVERESREAR"
     gene            complement(2430..3449)
                     /locus_tag="VLA60_17495"
     CDS             complement(2430..3449)
                     /locus_tag="VLA60_17495"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HSF11210.1"
                     /translation="MNQNELEQWILDLPKVELHLHIEGTLEPELMFDLAKRNGLSLPF
                     ATSEELKQAYNFQDLQSFLNIYYEGSRVLRQERDFFDLTWAYLTRVVKEHVRHVEIFF
                     DPQAHMNRGVDLHTVISGIHAALEQAHTELHISSKLIPCFLRDLPPDSARQTLGKILG
                     YRDWVSAVGLDSSEKGRPPELFQDVFDRARTEGLLTVAHAGEEGPPEYIWQALELLKV
                     ARIDHGIRCVEDESLVQHLAQTQIPLTVCPLSNVKLKVFPSLGHHNLKVLLQRGLCVS
                     IHSDDPAYFGGYITDNYLAAQRALNLQPIDILQLAMNGVKASFLSDTGKEKLTGEIQC
                     HATRL"
     gene            complement(3823..4251)
                     /locus_tag="VLA60_17500"
     CDS             complement(3823..4251)
                     /locus_tag="VLA60_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11211.1"
                     /translation="MKRFLRSFSLARAASIIMPWVLLLYGSGGQAAGPASPATLQSPV
                     VPQQGYSPPGYNPPQPGYEYVPNPQHNIYRPFEENYGGPTYIQQKAQAPYVTQYGESV
                     LEEWQYSGGYQGALDRGEIHHQPGSGSNSHIAPPTQTRGQ"
     gene            4614..4820
                     /locus_tag="VLA60_17505"
     CDS             4614..4820
                     /locus_tag="VLA60_17505"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HSF11212.1"
                     /translation="MKFTITITRDEDGVYIADCPAIPGCMSQGKTEEEAQGNIHEAIK
                     DCLEVRSEKGMPLTLTTREIEVTV"
     gene            5108..5323
                     /locus_tag="VLA60_17510"
     CDS             5108..5323
                     /locus_tag="VLA60_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HSF11213.1"
                     /translation="MKITATDALELSVPERIQLVTEIWDSIAECPEQIELTDATRQLL
                     RKRLAAYRANPGAGSPWEDVKRRILNG"
     gene            5320..5622
                     /locus_tag="VLA60_17515"
     CDS             5320..5622
                     /locus_tag="VLA60_17515"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HSF11214.1"
                     /translation="MSRKLIVTKEAEVDILHGYLSYEEKQDGLGRRFMDEIKFACDRI
                     LPNPFLYQEVEPDIRRAVTHKFPYLVFYTFDELTVQVLAVIHGSQDSTYINQRLDA"
BASE COUNT         1395 a         1356 c         1481 g         1442 t
ORIGIN      
        1 atgccgaagt gccaggggtg catcgcgaag tataattgcg caggagactg ctatgccagg
       61 agtgcttcct cgacgggaga cccggcttcg gcgggataca cggaacggtg tcatatcaca
      121 cgagaattgc tcaaagataa tctcctcctt gcactcatat ttaaaatggc cggtgtaaac
      181 gcgggtgggt cagtccaaca tacttgtccc atgtaatttt tgaaacgtgg acactatttt
      241 tcagaggggt catttcagtc agacaggcct gatcggaatg cggcaggata gagtattcga
      301 atagaggaaa ttctgattgg gattcggtta agagggttgt tcacaaatcc tttagaatga
      361 gccatgccga tcaatcgatt ctacacgtcc tcgctacaat accctgccgt gatcagaagc
      421 tggagtatca gctgcagaat ggccagaatt gataccagtg tcgacatctg aaccgatgtt
      481 cgcattgacg caactgagcg ccgtattgcc tggcttgcca atccacacga ttcgcgatac
      541 gcagaacctt cggcttacgc ttgtagctcc cacgcctata gccgctgcgc ccttcatggt
      601 aagccagata gaggcgcttg ggatcccatt tggaaatgcc gagctctctg ctgcttttat
      661 tgttgtacca gccgatgaag tccaaggcgt cctccatggt ggagcggcta ctgaagacac
      721 caccggcctc atcctcatat tcctcccatg tctcatcttt agcttgggca tagccttctg
      781 ccgacgattg gggtccgagt ggaatgaaca ggaaccaatc ccatggggga gtcgcgtcgt
      841 ggcgaaacgc cgactcctgt ttgacgaagg ccatgagaat gtgtggtggg gtgccccatt
      901 tttcttggga ttctttagcc gcatcgtacc actcggggta ttggtcgaat attgtgcaca
      961 gattctcctg atgcctgggg ggcattgatg cgcatcccgc taacagtgca atggccatcg
     1021 ccagcagaca agaaatcact cgcgtcgaaa tcatatggtt gtctggaatt caaatggaaa
     1081 tgggtatctt agtaaatgca attcgaacgc cacagggcta ttcgacgagg cagtcgggtg
     1141 attcctcgca tggttgtacc attcacgaat gctgaaaatt gtcagtgctc gttcgtcggt
     1201 ggaaaaagtt ctttccaata aatggcgccg ctcagtgaca ccatccggtt cagcgccctt
     1261 cttttgaccc tcgcatggac cataaccgca tggggctcac agtcgtcagt atatcaatgg
     1321 tacaaatatt ttttcgacag cgagattgaa tgttttccat atcttctttt ctcacatgta
     1381 ccctccaatc ctccattgtt ttttatcctg gtgatgctac ctcatttggt gtgaagtttt
     1441 gggaatcgga ctgggaagga gagacacgtg gagagcgttc ggaggcttag tgggccgtct
     1501 tcgagaagct gagccttatt gatgcgtttg acgcgaataa actgtatggt ctttccccgg
     1561 tttcgtgatg gcacattctc accttctgat aaaagccaaa ccatattaag aatctgtact
     1621 gtaagtcagt caggtcctag gggtaggcct ttctaggctt gtggacattt tttgtctcct
     1681 cggcttgcag tgaccatgta gaataatcaa gaatctcatg acacacgaat tgatagaagg
     1741 gacttcgtgc ttcctcattc tgtcctgatt gatgagaaaa ccattgagac gcgggttggc
     1801 gaattagcca aactgatcgc aaccgatttg ccgacgcgac agccaatcct ccttggattg
     1861 ctgacaggga gctttgtctt cctggctgat ctcgtgcgac acctttcaca ccatggtatc
     1921 gagccacaag tcgaattttt aaaagtttca cattatggtg acaccagtga ggccagcggg
     1981 tcagtcagct ttctgaggga gaaacttcct catatcacag atgaagtggt agtggtcgtc
     2041 gatgatattt tcgattcggg gttctcgttg cacgcggtgc atgaacatct gaaacaacag
     2101 cagccggcct gggtgcgttt ctgtacccta ttggataagc caagccgcca tcaggtggct
     2161 ctcagggtag actacgtcgg atttgagatt ccggatgtct gggtaatcgg gtatggcttg
     2221 gacctaggag gtgaggggcg ggctttacct tatttggcgg ctgttgagcg ggaaagccgg
     2281 gaagcccgct aaaataaatt tttatggcaa acgaaagttc tgtagagtga gtcccgccca
     2341 gtaggaaaag atccagacga ttagaactga atgcctgcca cgaagtctgc cggagaaaac
     2401 cccaataggg gcttttaaaa ataggaatgc taaagcctag tggcatggca ctgaatttca
     2461 ccagtaagtt tttcctttcc tgtatctgac aggaaactgg ccttcacgcc attcattgcc
     2521 aattgaagga tatcgattgg ctggagattg agcgcacgct gagcggcaag ataattatcg
     2581 gtaatgtacc ccccaaagta ggcagggtca tcggaatgga tcgacacgca taaacctcgt
     2641 tggagaagca cctttaaatt atgatgaccc agtgagggga acactttgag tttcacattc
     2701 gagagagggc aaaccgtgag aggaatttga gtttgtgcca gatgctggac cagtgattca
     2761 tcctcaacac aacggattcc gtggtcgatg cgggcgactt tgagtagttc gagggcctgc
     2821 caaatatatt ccggaggtcc ttcctccccg gcatgagcca cggtcagcaa tccttcagtc
     2881 cgtgcgcgat cgaagacatc ctgaaaaagc tccggaggac gccccttctc ggaagaatcc
     2941 agccccacag ccgataccca gtcacgatag cccaggatct ttcccagcgt ctgtctggca
     3001 gaatcgggag ggagatcgcg gaggaagcag ggaatcagtt tggaagaaat atgcagttcg
     3061 gtgtgagctt gttcgagtgc ggcgtgaata cctgaaatga cggtgtgaag atctacccca
     3121 cggttcatat gcgcctgggg gtcaaaaaag atttctacgt ggcggacatg ttccttcacc
     3181 acgcgtgtga ggtaggccca ggtcagatca aagaaatcac gttcctgtcg taaaacccgc
     3241 gagccttcat agtaaatatt caagaaggac tgcagatctt gaaagttgta agcctgcttc
     3301 agctcctcac ttgtcgcaaa ggggagagag aggccgttac gcttcgcgag atcgaacatc
     3361 aactccggct caagcgtccc ttcgatatga aggtgaagtt cgactttggg aagatcaaga
     3421 atccattgtt cgagttcgtt ctgattcatg gattaacctg agttttggga gtggcccatc
     3481 ctgaaaatga tcttcccaat tttccacaca acatgggaat tcatacggtc gcttgagata
     3541 ggtggtttaa aaaggtcccg gatcggtcgg tatttcttat ggatttttcc cttcactatt
     3601 gggccgttgt tcaatgtctc ccttcctgat tttgatggcc aaaattctta tgcccgatcc
     3661 ttctccatcc gcatcttcac gcggagacac ctaaattgca ggtccctgag ctgttccatc
     3721 ctggccatga gatcaggaat tttttaaaaa aggggttgaa gatgtgccac gactgaatgg
     3781 gttttttgac cctcattccg agtgttcaaa aaatctgttc gatcattgtc ccctggtttg
     3841 agtgggggga gcaatgtgac tgttgctccc ggacccgggt tgatggtgga tttctccacg
     3901 gtctaatgct ccctggtagc caccggaata ctgccattcc tcgagaacgg attccccata
     3961 ttgggtcacg tagggagctt gagctttctg ttgaatgtag gtcggccctc cgtaattttc
     4021 ttcaaacggt ctgtagatat tgtgctgcgg gttgggaacg tattcgtacc ctggctgagg
     4081 cggattatag ccgggagggg aatagccctg ctggggaacc acgggggatt gtagcgtggc
     4141 gggagaggcc gggcctgccg cttgtccacc gctcccatag agcaacaaga cccacggcat
     4201 tataatagaa gctgccctcg ccagggagaa agaccggaga aatcttttca ccaccatgat
     4261 gctccttaat caataaggtt aaaagtggtt cctccgaaac aatttgaacg tgcgacccac
     4321 taaaaaaatc gcatactgaa agaagtgaag tcaacgacat ggaacgacgg catgccggtt
     4381 gttgtgatga tcattttcgc actacgtcct ttagttctgt ccgactcaca atgtcttgcc
     4441 catcaacaca ataggtgggg ccaagttaat tggtgatgat ataactcatc ctggcttgac
     4501 agatgacgta taaacagctg tttgtttttc cttaccgtgg atatcggatt tcaatcggtc
     4561 aaattttgct ttttatttcg ggatgtcgga tatttgtgag agggggaaaa tcgatgaaat
     4621 ttaccataac gattacacga gatgaagacg gtgtgtatat tgccgattgt cccgccattc
     4681 caggctgcat gagtcagggc aaaaccgaag aagaagccca gggaaatatt catgaggcca
     4741 tcaaagattg cctggaggtg cgatcagaga aagggatgcc gttgactctg acaacgcgtg
     4801 agattgaagt cacagtgtaa tgccaccggt ccctgttctc tgtcctgccg cggttgtcaa
     4861 aacctttcaa aaactgggct gagaggtcgc ccattaacgc gggagccaca ttattatgac
     4921 caaaccttgc catctcgcaa ctctttccac ccctgaccat tctgaagtcg cgcggggaac
     4981 tcagtatacg tggctcgaag cgattggcct gcggcccccg gctcaagcgt gaactccggt
     5041 aggcgccagg gtagcttgtg gacgccagta tctggaacat ttttgaatat tgtgctattt
     5101 tcaagtcatg aaaatcactg ctactgatgc cctcgaattg tcagttccgg aacgtattca
     5161 actagttaca gaaatctggg acagtatcgc cgaatgccca gagcagatcg aactgactga
     5221 tgccacacgg cagcttctcc gaaaacggtt ggctgcctat cgggcgaatc caggcgctgg
     5281 atcgccgtgg gaagacgtta aacgccgcat tttgaacgga tgagtcgcaa gctcatcgtt
     5341 accaaagagg cggaggtaga cattctccac ggctacctct cctatgagga aaagcaagac
     5401 ggtcttggca ggcgatttat ggacgaaatt aaatttgcct gcgatcgaat cctaccaaat
     5461 ccatttctgt atcaggaagt agagccggac atccgaagag cggtcacaca taagtttccg
     5521 taccttgtgt tctatacttt cgacgagctg accgtacagg tactcgcggt catccatggc
     5581 tctcaggact cgacctatat caaccagcga cttgatgcct aaccacggcg acgaagcgac
     5641 ggcgcatctg cgggccgtga gacggtgaca atct
//
LOCUS       DATIAL010000577         6019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_426597, whole genome shotgun sequence.
ACCESSION   DATIAL010000577 DATIAL010000000
VERSION     DATIAL010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426597"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="VLA60_17520"
     CDS             complement(<1..1771)
                     /locus_tag="VLA60_17520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HSF11215.1"
                     /translation="MAKRTRSGTDSSNNNPSEIIPVLLDETTRQRYLNYALSVITSRA
                     LPDIRDGLKPVQRRILFSMFHNHRLVPDRPPIKSAKVVGSVIGEWHPHGDAAVYDAMA
                     RMAQPWSLRNTLVDGHGNFGSQDGDRPAAYRYTEARLTPLALELLNELNKNTVDFQPN
                     YDGKAEEPLVLPARFPNLLVNGSTGIAVGMATNIPPHNLGEVIEAAIALIANRDASIS
                     DLMKYLKGPDFPTGGEILNNKTEIREMYETGQGSIRLRGEYEVKALDHGKSAILITSI
                     PFAVDKSTIVERIGELIITKKVPQLVDVRDESTTEVQIALETQKDADPHLAMAYLFKH
                     TPLQNNFSVNLTCLVPVPGSIVARPDCLNLKQMLEQFIDFRFVTVKRRFSYDLQVLEK
                     RIHILNGFKLIFDALDQVLKIIRQSEGKADSANKLQQRFGLEPIQTDAILETPIYKLS
                     RTEIKKMLDELADKLRQAKNLKTLLASNTKLWDVVKKELEEVATAFGDKRRTKIGRRQ
                     TEEVEFDPDAFRVKEDAVITVSADGWIRRVGLIKDLSKARIREGDRIMTILGGSTLES
                     VVFFSNYGSAYSIRIGDIPPARSG"
     gene            complement(1855..3288)
                     /locus_tag="VLA60_17525"
     CDS             complement(1855..3288)
                     /locus_tag="VLA60_17525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HSF11216.1"
                     /translation="MSKKGLLFIIVLALIAIYLGLIGNARWGDSTAPEISLEQPFDLV
                     GPTTPLVLRIHDDETGLRDISVRIIHNMDTFSLADQSFSSEGWFSAAGGRDHEFTLNI
                     VPYANSDLPRRQGQAQLIVTARDYSWRNWFEGNGQRLEKEFTPQFTPPRLELLSPPTT
                     IVQGGAGIVRYRVIPEVSIHGVKIGKAFFPGYPAPDKAGMFAFIAFPYNLPSSTPIQV
                     IADDGYGNSALMDVSFKVKPQAWRTRSITISDSFIRKTVPTILAQTPELQSLGDPLKD
                     FLLVNDTLRKMNNETISKLAKSSRHEFLWKGAFRQLSGSQVEASFADHRKYTYKGQVV
                     DTQDHLGFDLAVTQHYPVEAANNGIVIFAGYLGIYGNTIILDHGYGLLSLYAHLSSIE
                     GKPGDAVTLGQPIGKSGTTGLAAGDHLHFSLILHGEQVNPTEWWDPFWVKTRIKDQLG
                     LPSLVKPTDEEPEPATTHLGPTPAPVQ"
     gene            3760..4788
                     /locus_tag="VLA60_17530"
     CDS             3760..4788
                     /locus_tag="VLA60_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HSF11217.1"
                     /translation="MPGLSDILGWFSSDLAIDLGTATTLVYVRGKGIVLSEPSVVAIE
                     KQSNTVLAVGVEAKRMVGRTPGNIVAIRPIKEGVIADFAMTERMLHYFITKAHNRRAF
                     VRPRCIIGVPSRITEVEQRAVRESASQAGAREVYLIEEPVAAAIGAGLPITEPSGNMV
                     VDIGGGTTDIAVISLGGIVCSESVKVAGDSMDDAIMSYIKRRYNLLIGDHMAEKVKIE
                     VGSAYPLEQAKTMMVKGRDMISGIPRTVVVNDSEIREALEDPIHAIVNALRNALENTP
                     PELAGDIIDKGVVITGGGSLLPGIATRFQEETNLPIITVEDPLTSVVYGVGKALDEVD
                     LFQKIQKF"
     gene            complement(4796..>6019)
                     /locus_tag="VLA60_17535"
     CDS             complement(4796..>6019)
                     /locus_tag="VLA60_17535"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HSF11218.1"
                     /translation="GQSLFRSIQSRNRPSHLPAALHRHLGPVMMGIVIGIAVGLWATS
                     SWSLSGKPAPVKGPIGLQPLVEHDFSKPVFLTASPDQTNRLFVVEQNGRIIIVTPGQR
                     NPSLFIDIAEKLSTGGERGLLGLAFHPQYSKNGRFFVNYTRAQDGATVIAEYHVSSDP
                     ARAEPQETIRLVIPQPYSNHNGGMIAFGPDAFLYIGTGDGGAGGDPENRAQDRKSLLG
                     KFLRIDVDGPPPYRIPADNPFVGQPGQPEIFAIGLRNPWRFSFDRQTGDLWAGDVGQN
                     LWEEIDVIAKGKNYGWRLLEGNHCFNPAKNCESAQDVVTPVTEYRHEQGRCSVTGGYV
                     YRGKRVPSLEGVYVFGDYCTGEIWGYRDGQTTQLLDTELRISSFGEDREGELYVIGHQ
                     GKIFQIAPNASVVLP"
BASE COUNT         1403 a         1470 c         1654 g         1492 t
ORIGIN      
        1 agccacttcg ggccggtggg atatcgccga ttcgaataga gtaggcgctg ccataattgg
       61 aaaagaacac caccgattca agggtgctcc cgcctaaaat cgtcatgatc cgatcgcctt
      121 cccgaatccg tgctttggaa agatctttaa taaggccgac ccgccgaatc catccatctg
      181 cggaaacggt tatcacggca tcttctttca cccggaacgc gtccggatca aactcgacct
      241 cctcggtttg ccgtcggccg attttagtcc gacgtttgtc tccaaaagcc gtggcgacct
      301 cctctaattc ttttttgacc acatcccata atttggtatt ggaagccaga agggttttga
      361 gattcttggc ctggcgcagc ttgtcggcca gctcatccag catttttttg atttcggttc
      421 gagataattt atagatgggc gtttccaaaa tcgcatcggt ttgaatgggt tccagtccga
      481 atcgttgctg cagtttattg gcagaatcgg cttttccctc actttgccgg atgatcttga
      541 gcacctgatc cagagcgtca aaaatgagtt tgaacccatt caggatatgg attcgctttt
      601 ccaggacttg caaatcgtag ctgaatcttc gtttgacggt gacaaagcga aagtcgatga
      661 actgttccag catctgcttg agattcaggc agtcaggacg ggccacgatc gatcctggta
      721 ccggaaccag gcacgtcaaa ttaacggaaa aattattctg cagcggcgta tgtttaaaga
      781 gataggccat ggccagatgg ggatcggcat ctttttgtgt ctcaagcgcg atttgaactt
      841 ctgtcgtgga ctcatcccgg acatccacta attgcggaac ctttttggtg atgattaatt
      901 cgccgatccg ttccacaatc gtggacttat cgacggcaaa gggaatggat gtaatgagaa
      961 tggctgattt cccgtgatcc aatgctttga cttcatattc tccgcgaagc cggatcgacc
     1021 cctgtccggt ctcatacatt tcccggattt cagttttgtt gttcaaaatt tcgccgccgg
     1081 tcgggaagtc cgggcctttc aggtatttca tgagatcgga aatagaggca tcccgattcg
     1141 caatcaaggc gatcgcggcc tcgatcacct ctcctaaatt atgaggaggg atgttggtgg
     1201 ccatgcccac ggcaattcct gttgatccat tcaccagcag gttgggaaag cgggcgggga
     1261 gaaccagcgg ttcttccgct ttgccatcgt aattgggttg gaagtcgacc gtgtttttat
     1321 tcagttcatt cagcagttcg agtgcgaggg gtgtcagacg ggcttcggta tagcgatagg
     1381 cggcgggacg gtcaccgtcc tggctgccga aatttccatg gccatccacc agggtgttgc
     1441 gtaatgacca gggctgggcc attcgagcca tcgcgtcata gaccgccgcg tccccatggg
     1501 gatgccattc accgataacg gaccccacca ccttggcgct cttgatggga ggacggtcag
     1561 gcacaaggcg atgattgtga aacatggaaa acaaaatgcg ccgctgcacg ggtttcaatc
     1621 catcccggat atccggaagg gcccgtgagg taataaccga gagcgcataa ttgagatagc
     1681 gttgccgcgt cgtctcatcc aacagaaccg ggatgatttc tgacgggttg ttgttggaac
     1741 tatcggtgcc agaccgggtt cgtttggcca tacgttcgta tcctttgata actcagaagg
     1801 tcttgaaatt catgtgggat tcggtaactc ggagggtgaa gccgccttat gacattactg
     1861 gactggcgcg ggggtggggc ctaagtgtgt tgtggccggt tccggttctt catccgttgg
     1921 tttaaccaaa gagggcaatc ctaactggtc tttgatacgg gtttttaccc aaaacggatc
     1981 ccaccattcc gtgggattca cctgttcgcc atgcaaaatc aaactaaaat gcaggtgatc
     2041 gccggctgcg agaccggtgg tacctgattt gccaatgggt tgccccaggg tgacagcgtc
     2101 accgggtttg ccttcaatcg aggagagatg cgcatacaag gaaagcaatc catagccatg
     2161 atcaagaata atcgtgttcc cataaattcc cagatagcca gcaaatatca caattccgtt
     2221 attagcggct tcgaccggat aatgctgggt gacagcgaga tcaaacccta aatgatcttg
     2281 ggtgtcgacg acctgtccct tatacgtata tttgcggtga tcggcgaagg aggcttcgac
     2341 ctgtgagccc gacaattggc gaaacgcccc tttccagaga aattcatggc ggctgctctt
     2401 ggccagtttg gaaatggtct cgttattcat tttgcgtaac gtgtcgttga ccagaaggaa
     2461 atccttcagt ggatcgccca gactctgtaa ttcaggggtc tgtgccagaa tggtcggaac
     2521 cgttttacga ataaatgagt cggaaatcgt gatggaacgg gtacgccagg cttgaggttt
     2581 gaccttgaaa ctcacatcca tgagagcgga gttgccatac ccgtcgtctg caatcacctg
     2641 aatcggggtc gaggacggta aattataagg aaaggcaatg aaggcaaaca tgcccgcttt
     2701 gtccggggca ggatatcccg ggaagaaggc ctttccaatc ttgaccccat gaatagacac
     2761 ttccgggatc actcgatacc tgacgatacc tgctccacct tggacgatgg tcgtgggggg
     2821 ggacaggagt tccaatctgg gtggtgtaaa ttgtggggtg aattccttct ccaatcgttg
     2881 tccgttacct tcaaaccagt ttcgccagga ataatccctg gcggtcacaa tgagttgggc
     2941 ctgcccttgt cgtcgaggca ggtccgagtt ggcataggga acaatgttca gcgtgaattc
     3001 atggtcccgt cctccggctg ccgaaaacca cccctcagaa gaaaaggatt ggtcggccaa
     3061 actgaatgta tccatgttgt gaataatgcg gactgaaatg tcccgaagac cggtctcatc
     3121 atcatgaata cgaagaacca gtggtgtggt ggggccgacc agatcgaacg gttgttcgag
     3181 agagatttca ggggcggtgg aatctcccca tcgtgcgttc ccaattaatc ccaggtaaat
     3241 ggcgataaga gccaggacga tgataaacag caagcctttt ttagacacga tcgaggtaac
     3301 tcttcctcca gaggatgatg cgagcaggac aaaaaatgtt cctcttctta taccataccc
     3361 agccttcaga gcaagtgtgt gatctgtctg aagaggcggt gaagcgcccg cgcctgattc
     3421 acaaccgtag aaatgggttc tctgcactct tgacatggac aggatgatcg cctagcctgt
     3481 gccgggccac agccaggcct catgtctcat atccctcctc tcgggccatg tatctcgtcc
     3541 agatggatga tgtgggaatt cccgctccct gaatggtaga tcaggagctt ggtttcatgg
     3601 aagaagccgg tggcttcttg gtgacaaaca cattcccgaa cattttctca atattccttt
     3661 attttgacaa atggtgttcc gatatatgtc ggtagtgccg aacaggcgac agcctgatcg
     3721 gcattttctg aatcaaccgt ctttccacat ggggggctca tgccagggtt gagtgatatt
     3781 ttagggtggt tttcgagtga tttagccatc gatttaggaa cggcgacgac actggtctat
     3841 gttcgtggta aaggcatcgt cctgagtgaa ccctcggtgg tggccatcga aaaacaatcg
     3901 aacacggtct tggccgtggg tgttgaagcc aaacgcatgg tgggaaggac gccgggtaat
     3961 attgtggcga tccgcccgat caaagaaggg gtcattgccg atttcgccat gaccgagcgc
     4021 atgttgcatt attttattac caaagcgcat aaccgacggg ccttcgtgag gcctcgctgt
     4081 attattggtg tgccatcccg tatcaccgaa gttgagcaac gggccgtccg tgaatcggcc
     4141 agccaagccg gcgcccggga agtttatctc atcgaagagc cggttgcggc agcaattgga
     4201 gccggattac cgatcacgga accttcgggg aacatggtgg tcgatattgg aggaggtacc
     4261 accgatattg ctgttatttc ccttgggggc attgtctgca gcgagtcggt gaaagtggca
     4321 ggtgattcca tggatgatgc cattatgagt tatatcaagc gacggtataa ccttctgatc
     4381 ggtgatcaca tggcggaaaa ggtgaagatc gaagtcggat ctgcctatcc gttagaacaa
     4441 gccaaaacga tgatggtcaa agggcgggat atgatttctg gtattccccg gacggtagtg
     4501 gtgaatgatt ccgagattcg agaagcattg gaagatccca tccatgccat agtgaatgcc
     4561 ttacggaatg cgttggagaa tacgccgccg gaattagccg gagatattat cgataaggga
     4621 gtggtgatta caggcggggg gtcgttgttg ccgggaattg ccacgcggtt tcaggaagaa
     4681 accaatctgc ccattattac ggtggaggat cctttgacct ccgtggtgta tggcgtggga
     4741 aaagctcttg atgaagtcga tttatttcaa aagattcaaa aattttaatg gttggctagg
     4801 gcaggacaac cgaggcgtta ggtgcgatct gaaaaatctt accttgatga ccgatcacat
     4861 agagttctcc ctcacgatct tccccaaaag acgagattcg tagttccgta tccagcagtt
     4921 gtgtggtctg cccgtcgcga taaccccaga tttctccggt acaataatcc ccgaacacat
     4981 agaccccttc taaggaaggc acgcgtttcc cacgatagac atagcccccg gtcaccgagc
     5041 atcgtccctg ttcgtgccgg tattctgtga ccggcgtgac cacatcctgc gccgattcgc
     5101 agttcttggc gggattgaag cagtggttcc cctctaagag tcgccatcca tagtttttcc
     5161 cttttgcaat cacatcgatt tcttcccaaa ggttctgtcc gacatccccg gcccataagt
     5221 ccccggtctg gcggtcaaag gaaaagcgcc agggattgcg aaggccaatc gcaaagattt
     5281 caggttggcc cggttgtccg acaaagggat tgtcggcagg gatgcggtag ggaggcggtc
     5341 catccacatc aatccggagg aatttaccca gcaatgactt tcgatcttgc gctcgattct
     5401 ccggatcacc gcctgctcca ccatctcccg ttccaatata caaaaatgca tcggggccaa
     5461 aggcgatcat gcctccgtta tggttgctgt agggttgggg aatgacgagt cggatggttt
     5521 cttgtggctc cgctcgggcg gggtcggatg atacgtggta ttcggcaatc accgtggccc
     5581 cgtcttgtgc tctggtgtag ttaacaaaaa accggccatt tttcgagtac tgaggatgga
     5641 atgccaggcc caggaggcct cgttcccctc ccgtggacag cttttcagcg atatcgataa
     5701 aaagagacgg attccgttgg cctggagtca cgatgataat gcggccattc tgttctacca
     5761 cgaacaagcg attggtctga tccggactgg cggtgagaaa gaccggcttg gaaaagtcgt
     5821 gttccaccag gggttgcaat ccaatggggc ctttgacagg ggcaggtttt ccagaaagac
     5881 tccagctcga ggttgcccaa agccctaccg ctatccctat gacgattccc atcatcacgg
     5941 ggcccaggtg tctatgaagg gctgcgggaa gatgggatgg tcgatttcgg gattggattg
     6001 agcggaatag ggattgccc
//
LOCUS       DATIAL010000578         6589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_747123, whole genome shotgun sequence.
ACCESSION   DATIAL010000578 DATIAL010000000
VERSION     DATIAL010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747123"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..350)
                     /locus_tag="VLA60_17540"
     CDS             complement(138..350)
                     /locus_tag="VLA60_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11219.1"
                     /translation="MLGLLVGFYVSDEVKMPVVISACLGVAIALGMSGISSAYISEAA
                     EKRRELKELEKAMVADLRQSAHGRAA"
     gene            complement(458..736)
                     /locus_tag="VLA60_17545"
     CDS             complement(458..736)
                     /locus_tag="VLA60_17545"
                     /inference="COORDINATES: protein motif:HMM:NF014710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF211 domain-containing protein"
                     /protein_id="HSF11220.1"
                     /translation="MVQIKRIVLNVLKPHNPNALDFTTAIAEKMPGCRIKLTVAAMDE
                     KTETVLLMIEGENVPFNAIAEAISSLGGSIHSIDEVDVENGPVDTAPA"
     gene            1060..1626
                     /locus_tag="VLA60_17550"
     CDS             1060..1626
                     /locus_tag="VLA60_17550"
                     /inference="COORDINATES: protein motif:HMM:NF015782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein"
                     /protein_id="HSF11221.1"
                     /translation="MTMKLKNLIWSLVLALGSAGGCASSPVEIPEILQNQIDPTLTFS
                     DLIQHPGAYQGKMLVLGGEVLSAKRLAEGTRLEVLQLPLDEYQRPVATRTDSQGRFIA
                     MEKAFLDPAMFPANTQVTLVGEVTGTVSAKLDEMDYQFPALVIRYLYVWKEPALIQES
                     HSGPWYSIFGGGSTGGRVGGGVGVGIGF"
     gene            1915..2601
                     /locus_tag="VLA60_17555"
     CDS             1915..2601
                     /locus_tag="VLA60_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HSF11222.1"
                     /translation="MAFANKQRREMDKEQEELTSYVNSEGEGRAQRMFGTSRRATAFY
                     RNQMTNELNDKMQSFIARQEMVFLSTANANGECDCSYRVGTAGFVGVLNAKTLAYPEY
                     RGNGVMASVGNILENPRIGLLFIDFLQSTVGLHVNGHARVVTSEEVAAMPEIPQAMLD
                     ATHIKGGQHPECWVIVEVEEAYIHCSKHVPLMKKVEKELHWGTDDERHKGGDFFQVQS
                     DRSNTFSSEE"
     gene            complement(2836..>6589)
                     /locus_tag="VLA60_17560"
     CDS             complement(2836..>6589)
                     /locus_tag="VLA60_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF11223.1"
                     /translation="GPTTAGLIGTEEFLFDAHEQGWLWAFFFFQMVFCGTATTLVSGA
                     VAERMRFTGYLVTSLIVSLLIYPILGHWMWGGLATGQNTGWLNQLGFIDFAGSTVVHS
                     TGGWVALAAILVIGSRHGRFSDKAAPIQGQNLPLATLGVLLLWFGWYGFNGGSSLEFT
                     QKASQILVNTTLAGATGALTALGLSWYYLKRPDVGHALNGVLAGLVSITASANIMSPE
                     GTILIGVGAGIICVGATIILERLHIDDAIGVVPVHACVGVWGTIVFPFLSTPDSWGTG
                     LTLWEQVGIQILGSTVCFFWAFGIGFAILWLINQWVPLRVTAEEERIGLNMAEHGAGT
                     AILDLLVQMEEQRNSHDFTRHVLSEPHTEVCQIAEEYNRVLDTINTEQRRRQEMDAAL
                     AYGARLDTLFQEIAKATNESSTIEDAMQLTMDLICQNTGWPVGHLYLLSEEKDRLLIP
                     TAIWHLEHPERFETFRSITERTDFQPGEGLPGRVLASGKPGWIRDVAQDPNFLRPQQA
                     KDIGVKAGFGFPIIIGEQVVGVLEFFSTERMEPDAKLLQVMGYVGAQLGRVVERKRGE
                     AILIQAKAEAEAATKTKADFLATMSHEIRTPLNGIIGMSDILLGTHLSEDQQDCLLTI
                     KDSGGALLTIINDILDFSKIEAGKLTLETIDFDFRETVDAVVDLLGLKAQEKGIELIS
                     LINPEIPTTVQGDPVRLRQILMNLIGNAIKFTAQGEIVVQVVPAEESSGTVLLRFMIT
                     DTGIGITHEGQGRLFQAFSQEDSSTTRKYGGTGLGLSISKMLAELMGGTIGVHSDLGQ
                     GSCFWFTIRLGTQQSHPIFDKILPRELEGVRIGLIDDNATNRMLLQKYTSYWGMQSIQ
                     AENGEKALASFRERALNGELCQLALIDMNMPGMDGLELAKAIKADPQLATMRLILLNP
                     LVNRIENIHRLNQKHISAFVNKPVQYKQLHQCLLNVMRRSNVAPSPNTIQTLKNEMNK
                     GSGQRLLLVDDNLVNQQVGVRMLTTLGYQVDMAANGREAVEAVTRTAYAGVLMDCQMP
                     EMDGFEATREIRKGERTTPQLPIIAMTATAMIGDREKCLEAGMNDFLSKPVKLEDLRC
                     VLEKWLFQASSTRNHEGETESHQPDNPEASLRSGTSAPDPSPPLDSATLADLRGLGGD
                     EDPAFFISVIEQFCQDAVTHMDGINLAIQENKGESLRKVAHAFKGCSRTIGAKSLAEL
                     AFQLEQMGHTQNLEKAQAILMALQSEFERVQAALQDELKQSAATLS"
BASE COUNT         1572 a         1639 c         1720 g         1658 t
ORIGIN      
        1 aaggccagga caaatgccga ttccagcgga ggaagagcta gtggcttcag cagtggagct
       61 gcccacaagg gcagcaggat caccagtgag atggccaagg gagcgatccc atttacggca
      121 atgacgaccg gcaggagtca agcggctcgt ccatgggctg attgcctcaa atcggccacc
      181 atggcttttt caagctcctt gagttctctg cgcttttccg cagcctcgct aatgtaggcg
      241 ctactgatgc cactcatccc cagggcaatg gccaccccga ggcaggcact tatgaccaca
      301 ggcattttga cttcgtcgct cacataaaat cccaccaaca gacccagcat cgtgagtgcg
      361 ccgtcgaatc cattgacgac aaagtaccgg cgtgggatga gatgggaacg tgtaagtgtg
      421 agaagtcagg taagctctgc aatcagggac gccatggtta agcgggtgca gtatcaaccg
      481 gtccattttc cacatcgacc tcgtcgatac tgtgaatcga tcctcccaga gaactaatgg
      541 cttcagcgat ggcattgaaa ggcacatttt ctccctcaat catcagcaag actgtttctg
      601 ttttttcatc catcgccgcg acggttaatt tgattcgaca tccaggcatt ttttctgcaa
      661 tcgctgtggt aaagtcaagg gcgttagggt tatggggctt gaggacatta aggacaatgc
      721 gtttgatctg aaccatcggg tatcgcgttc ctggatacat gagaacaaca cagccgtgac
      781 atgatatcgc actccatagg caatgtaacc gctctttttt cttaggaatc ggaaaagaaa
      841 gtgtagccgg attatgccga accgttttat ccggccttgc aacttgatgc tgtttggcgg
      901 caatccatct tgggggggaa taacaaagcg gtctcaggtg cgcagagtcc ttcttctgca
      961 ctgccatgat tcaagaggtc tctcgatcgg gttcatctgg aatgatgggt tgccggtcag
     1021 ataccacacc aaaatttatc catccgctta tgatagagta tgacgatgaa actgaaaaat
     1081 ctcatatggt ctttggtgct agctctaggg agtgccgggg gatgcgcgtc ctcgcccgtg
     1141 gagataccgg aaattcttca gaatcagatt gatcccacac tcacgttttc ggatctgatt
     1201 caacatcctg gggcctatca gggaaagatg cttgtgcttg gaggggaggt tctgtcagcc
     1261 aagcgtcttg cggaaggtac ccggcttgag gtgttgcaat tacccttgga tgaataccaa
     1321 cggcccgtgg caactcgcac cgactcacaa ggccggttta ttgccatgga aaaagccttt
     1381 cttgacccgg ccatgttccc ggcgaatact caggttactc tcgtgggaga ggtgacgggg
     1441 acggtttccg ccaagcttga tgaaatggac tatcagtttc ccgccctggt gatcaggtac
     1501 ttatatgtgt ggaaagagcc ggccttgatt caggaaagcc actctggtcc ctggtacagc
     1561 atctttggtg gtggcagcac cggaggccgg gtcggtggtg gggtgggggt aggtatcggc
     1621 ttttagtgtc gatttgctct ttccgtcata gcttttgctc cttcacgcat tcggctagtc
     1681 gtttggagat gaatcgaaat actaatcctc cgcattattc agtttttccc tctcacttct
     1741 ccgcagtatc tttaggtttc ccatacaata attgaaggtg tcccacttcc ctcatatttt
     1801 gaggtgtgat ggggcatgtg gttctttcct ttggcacgcc tgtgcgtcta agatatcgac
     1861 tgaatctccc tccagagatt ttcaataatt aatgacggca ggggaggacc tggcttggca
     1921 tttgccaata aacaaaggcg tgagatggat aaagagcagg aagaattaac atcgtatgtg
     1981 aattccgagg gggagggtcg agcccaacgc atgtttggga catccagacg ggctacggct
     2041 ttttatcgga atcagatgac gaacgaatta aacgacaaaa tgcagtcgtt tattgccaga
     2101 caggagatgg tttttctttc gaccgccaat gccaacggcg aatgtgattg ctcatatcgg
     2161 gtggggactg ccggattcgt cggagtgctc aatgccaaaa cgttagcata tcccgaatat
     2221 cgcgggaacg gggtcatggc gagtgtcggc aacattcttg aaaatccccg gatcggcctg
     2281 ctgtttatcg attttttaca aagtacggtc ggtctgcatg ttaatgggca tgcacgtgtg
     2341 gtgaccagtg aagaagtggc tgccatgccc gaaattcctc aggccatgct tgacgctacc
     2401 cacataaaag ggggacaaca tcctgaatgc tgggtgatcg tagaagtcga agaagcctat
     2461 attcattgtt ccaagcatgt tcctctgatg aaaaaagttg agaaggaact gcattgggga
     2521 acggatgacg aaaggcataa aggcggagat ttttttcagg ttcaatccga ccgatcaaat
     2581 accttttcat ctgaagaata gaaaaagtcc gtttggattg agaaggtata aggctcactt
     2641 caaggacgaa tagaaacctg ttggtctgtg aagggaaagg cacggaacgc ttttaaaaaa
     2701 taaccgggcg agacgagagt ggtgtatggt cattgtagac cgggcttggg gctagtcctt
     2761 gttcttaaag tcccgtctgg atagaaagcc catttttcgg caagactcag agggcttgct
     2821 tttcccatat gaccattatg aaagggtcgc agcggactgc tttaattcat cttgcagggc
     2881 ggcttgcacc cgctcgaatt cagattgcag tgccattaag atggcttgag ctttttcaag
     2941 attttgggta tgtcccatct gctcaagttg gaaggctaat tcggcaagtg acttggcgcc
     3001 aatcgttcgt gagcaacctt tgaacgcatg cgccaccttg cggagggatt cacccttgtt
     3061 ttcctgtatc gccagattga ttccatccat gtgtgtaacg gcatcctgac aaaattgctc
     3121 gatgacggaa atgaaaaatg caggatcctc atctcctcct aatccgcgta ggtctgcgag
     3181 agtcgctgaa tccaagggtg gggaagggtc cggtgcggat gttccggatc ggagtgaagc
     3241 ctccggattg tccggttgat gagattctgt ttcaccctca tggtttcttg ttgaagaagc
     3301 ctggaagagc catttctcca agacacatct cagatcttct aatttcaccg gtttggataa
     3361 aaaatcgttc atgccggctt ctaaacattt ttctcgatca ccaatcatgg ctgtggccgt
     3421 catggcaatg ataggcagct gtggggtggt acgttcacct tttcgtattt ctctggtggc
     3481 ctcaaatccg tccatctccg gcatttgaca gtccatgagt acaccggcat aggcagtccg
     3541 tgtcacggct tcaaccgctt cccgtccatt tgctgccata tccacctgat atccgagtgt
     3601 ggtcagcatg cgaacgccga cttgttgatt gacgagattg tcatcgacca ggaggagtcg
     3661 ctgccctgag cctttgttca tctcattctt gagcgtttga atcgtgttgg gcgaaggggc
     3721 cacgttagac cttctcatga catttaacag acattggtgc aattgcttgt actgaaccgg
     3781 tttgttgaca aaggccgaaa tatgtttttg attgagtctg tggatatttt cgatccggtt
     3841 caccaaagga ttgagtaaga tcaaacgcat ggtcgccaac tgcgggtcgg ctttgatggc
     3901 ttttgccagt tcgagaccat ccatgccagg catgttcata tctatgagag caagctgaca
     3961 aagttctcca ttcaaggcgc gttcccggaa tgaggccagg gccttctcgc cgttttccgc
     4021 ttggatactt tgcatgcccc aatagcttgt atatttttgg agtaacattc gatttgtggc
     4081 gttatcatcg atgagaccaa tacgcacacc ctcaagttcg cgaggcaaaa tcttatcgaa
     4141 tattgggtga gactgttgag tgcctaatcg gatggtgaac cagaagcaac tcccttggcc
     4201 caaatcgctg tggactccga tcgttcctcc catgagttca gccagcattt tggatatgga
     4261 gaggcctaac cctgttcctc cgtatttccg ggttgtggaa ctgtcttcct ggctaaaggc
     4321 ctgaaacagt cgaccctggc cttcatgggt tatcccaata cccgtgtcgg taatcatgaa
     4381 tcgtaagagg acggttccag agctttcttc tgcgggtaca acctgaacca caatttcacc
     4441 ctgagcggtg aacttaatcg cattgccaat gaggttcatc aagatctggc gaagtctgac
     4501 gggatcacct tggacggtgg tgggaatttc cggattgatc aagctaatca attctatccc
     4561 cttttcctga gccttaaggc ctaacagatc gaccacggca tccaccgtct ctcgaaagtc
     4621 aaagtctatc gtctcgagcg tcagcttgcc tgcttcaatt tttgagaaat cgagaatgtc
     4681 attgatgatt gtgaggagcg caccacccga atccttgatc gtgagcagac agtcttgctg
     4741 gtcctcgctg agatgtgtgc cgagcaatat atccgacatg ccgatgatgc cattcaacgg
     4801 cgttcggatt tcatgactca tcgtggccag aaaatcggcc ttcgttttcg tcgcggcctc
     4861 ggcttcagct ttggcctgaa tcaaaatggc ttctcctcgt ttgcgttcca ccacgcggcc
     4921 cagctgtgct ccgacatacc ccatgacttg taataatttg gcatccggct ccatcctttc
     4981 cgtcgaaaaa aactccaaca cgcctaccac ctgttccccg atgataatag gaaatccaaa
     5041 tccagccttc acgccaatat cttttgcctg ttggggtctg agaaaattgg gatcttgagc
     5101 cacatctcga atccaccctg gttttccact ggccaacacc cgaccgggaa gaccttcgcc
     5161 gggttgaaaa tcggtgcgtt cggtaatgga acgaaaggtc tcaaagcgtt cggggtgttc
     5221 caaatgccag atggcggtgg gaatgagtaa gcggtctttc tcttcagaga ggaggtacaa
     5281 gtgtccgaca ggccacccgg tattctgaca gatcaaatcc atagtgagtt gcatcgcatc
     5341 ttcaattgtc gaggattcgt ttgtggcctt tgcaatctcc tgaaagaggg tatcaagtct
     5401 ggccccatag gccagggcgg catccatttc ttggcgtcgc cgctgctcgg tattgatcgt
     5461 gtctaatacc cggttatatt cttccgcgat ctggcaaacc tccgtatgag gctctgagag
     5521 gacatgacgg gtaaagtcat gactgtttcg ttgctcttcc atttggacaa ggagatccag
     5581 aatggcagtg ccggcgccat gttctgccat gttgagcccg attcgttcct cctcggctgt
     5641 gacgcgcagg ggaacccatt gattgatcag ccagagaata gcgaagccta taccaaatgc
     5701 ccaaaagaaa cagacggtgc ttcctaggat ttgaatgccc acttgttccc agagggtgag
     5761 ccctgtgccc caggagtccg gagtactcaa gaaaggaaag acaatggtgc cccacacccc
     5821 tacgcaggca tggacgggga cgacaccaat ggcgtcgtca atgtgcaaac gttcaagaat
     5881 gatggttgcg ccgacacaga taatcccggc tccgacgccg atgaggatcg tgccttcagg
     5941 cgacatgata ttggccgaag cggtaatgct gaccagaccg gccaggactc cgttaagggc
     6001 atgaccgaca tcgggccgtt tcaggtaata ccagcttaag ccaagagccg tcagagcacc
     6061 ggtggctccg gcaagggtgg tattcaccag aatctgtgag gctttctggg taaactcgag
     6121 actactccct ccgttaaaac cgtaccatcc gaaccacagc agtaagactc caagcgtggc
     6181 taatggcaga ttctgcccct ggataggcgc cgccttgtcg gaaaagcgac catggcggga
     6241 tccgatgacg aggatggccg ccaacgccac ccaacctccc gtcgaatgga cgacggttga
     6301 gcccgcaaag tcaataaatc cgagttgatt caaccatccg gtgttctgcc ctgtggctaa
     6361 tcctccccac atccaatggc caagaatggg atagatgaga agagacacaa tgaggctggt
     6421 gaccaaatac cccgtaaatc gcatccgctc ggcaacggca cctgagacga gggttgtggc
     6481 ggtcccacaa aataccatct gaaagaaaaa aaatgcccac aaccatccct gctcgtgggc
     6541 atcaaaaaga aactcctctg tgccgatgag gccggctgtc gttggtccg
//
LOCUS       DATIAL010000579         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_532283, whole genome shotgun sequence.
ACCESSION   DATIAL010000579 DATIAL010000000
VERSION     DATIAL010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532283"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VLA60_17565"
     CDS             <1..1226
                     /locus_tag="VLA60_17565"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HSF11224.1"
                     /translation="RGMVKDGATVWGTRHRTSPDLAAFANGTAVRYLDCNDTYLSKEP
                     AHPSDNIPACLAVAESLHVHGRQFIQSIVLAYEIQCRLCDAAALRPRGWDHVTYGAFS
                     TAVATARLLKLPKKQIVHALNLAGITAGALRQTRVGEVSLWKACAFANASRNGIFAGF
                     AAREGMTGPEAMFEGEKGFMQVISGPFSLPEMGGRHGSSYKILDTYIKPHPVEYHAQS
                     AVEAAFAIREQLIAACGEFPVHRIKAIIVGSGDVAIEIIGRDQEKWHPKTRETADHSL
                     PFCVAVSLTDGEISPRSFSPHRLNDTVLLNLMQKIRVEEVKEFSERYPDAMANRIDVT
                     LMDGRTFTAQIDHPKGHPRRPLTDRELELKFQKLSSRKIGRKDRTKVIQTVWELDRLK
                     DVNVLVSALPVMDRR"
     gene            1223..2104
                     /gene="prpB"
                     /locus_tag="VLA60_17570"
     CDS             1223..2104
                     /gene="prpB"
                     /locus_tag="VLA60_17570"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HSF11225.1"
                     /translation="MSGNPITSQSRARRFRTELQRDTLAVPGVFNAFSARLVEDAGFP
                     AAYLSGAGLAASRALPDIGLLTMTEVVTETRYVTQRVQIPVIVDADTGFGERHQVYRT
                     VQELESAGAVGIQLEDQVLAKRCGHLPGKTLISCEGMCQKIQSAVEAKQDRDLVIIAR
                     TDARAVEGLDGAIERARAYRAAGADAIFPEALQSAKEFQTAAKALKYSRSILVANMTE
                     WGQTPYMTVKEFSGMGYHIVLFPMTVFRVMAKAGRAALLELQKQGTQQAFLDRMQTRQ
                     ALYEVLDYQKYERVPKE"
     gene            2215..>2748
                     /locus_tag="VLA60_17575"
     CDS             2215..>2748
                     /locus_tag="VLA60_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HSF11226.1"
                     /translation="METAVHNPGLDGVVVGESAICQIDETRGSLRYRGYAIEELAEQA
                     TFEEVAYLLLHGDLPSSRDLQEWQAELAHAGSLPPVVWQFLQTVPASANVMDVLRTGV
                     SFLGMIDPEAPDISHEANLRKAVRLLAKMPLLLSWAVRTRFDHPLFQAPATKSFAQSL
                     LYLLTGSLREDHAKELEV"
BASE COUNT          648 a          696 c          789 g          615 t
ORIGIN      
        1 cccgggggat ggtcaaagat ggcgcaacgg tatggggaac ccgccatcga accagtccgg
       61 atctggcggc ctttgcgaat ggcacggcgg tgcggtatct cgactgcaac gatacctacc
      121 tctccaagga gcccgctcat ccgtcagaca atattccggc ctgtctggcg gttgccgaaa
      181 gccttcatgt tcatggccgg caattcattc aatccatcgt gctggcctat gagattcagt
      241 gccgtctttg tgacgcagcc gcgcttcgcc cccgaggatg ggatcacgtc acctacggag
      301 cattttctac cgcggttgcc acagccaggc tgctcaagct ccccaaaaaa cagatcgtgc
      361 atgcgctcaa tctggcgggt attacggctg gagcactgcg tcagaccaga gtgggggaag
      421 tttccctctg gaaagcctgc gcgtttgcca atgcaagcag gaacggaatc ttcgcaggat
      481 ttgcggctcg cgagggtatg accggaccgg aggccatgtt tgaaggggaa aaggggttta
      541 tgcaggtgat ttccgggcca ttctctttac ccgaaatggg aggacgccac ggctcttcct
      601 ataaaattct tgatacctac atcaagcccc atccggtgga atatcatgct cagtcggcgg
      661 tggaggcggc attcgccatt cgggaacaac tgattgctgc gtgtggggaa ttccctgtcc
      721 accgcatcaa agcaattatt gtcggcagtg gtgatgtggc gatcgaaatc attggacggg
      781 atcaggaaaa gtggcatccc aaaacccgtg agactgctga tcatagtctc cccttttgcg
      841 tggcagtctc cctgacggat ggggaaatat ctccccggtc attttccccg catcgactga
      901 acgacaccgt tctcttaaat ctcatgcaga aaatccgtgt tgaagaggtc aaagagtttt
      961 ctgagcgtta tccggatgcc atggccaaca gaattgatgt caccctgatg gacggcagga
     1021 cgttcacggc tcagattgac catcccaaag gtcatccacg acgcccactc accgatcgcg
     1081 aactggaatt gaagtttcag aagttgtctt cacggaaaat cggccggaaa gataggacga
     1141 aggttattca aaccgtgtgg gaattggacc gtctgaaaga cgtgaatgtc ctggtgtcgg
     1201 cattaccagt tatggataga cgatgagtgg gaacccaata acctcccaga gtcgggcgcg
     1261 ccgctttcga accgagcttc aacgggacac gttggcggtg ccgggtgtat ttaatgcgtt
     1321 ttcggctcga ttggttgagg atgccggttt tccggcagca tacctgtcag gagcgggtct
     1381 ggcggcttcc cgggcactac ccgatatcgg tctgttaacg atgacggagg tggtaacaga
     1441 aacccggtac gtgactcaac gggttcaaat tccggtcatc gtcgatgccg atacgggttt
     1501 tggagaacgc caccaggtgt atcggacggt tcaggaatta gaatcagccg gggcggtggg
     1561 aatccaattg gaagaccagg tgttggcaaa acgttgcggc catttgccag gaaaaaccct
     1621 gatttcgtgt gagggcatgt gtcaaaaaat tcagtcggca gttgaagcaa aacaggaccg
     1681 ggatcttgtc atcattgcga ggacggatgc cagggctgtg gaaggtctgg atggggccat
     1741 tgagagggct cgagcttaca gggcagccgg cgccgatgcg attttcccgg aggccctgca
     1801 gtcagccaaa gaatttcaaa ccgcagccaa agccctgaaa tattccagga gcattctggt
     1861 cgccaatatg actgaatggg gacaaactcc ctatatgacc gtcaaggaat tctccggaat
     1921 gggatatcac atcgtgctgt tccccatgac cgtatttcgt gtgatggcga aggcaggccg
     1981 agcagctctc ctggaattac aaaaacaggg gacccaacaa gctttcctcg accgcatgca
     2041 aaccaggcag gcgttgtacg aggtattgga ttatcaaaag tatgaacgtg tgccaaagga
     2101 atgaaaaaag gcgcgttgaa ccttccaaat cctgggaatg catctgacgg cctctttgga
     2161 ccgcctcagg gcacaaaatt ttcaactggc ctgtaaaaga aaggcaggtg tgtcatggaa
     2221 acggccgttc ataatcccgg gctggacggg gtggtggtgg gggaatcagc catctgccaa
     2281 atagacgaga cgcggggaag tttacgatac cgaggatatg ccattgaaga gctggccgag
     2341 caggcgacgt ttgaagaagt ggcatattta ttactgcatg gagatctgcc gtcatcccgc
     2401 gatcttcagg agtggcaggc cgagttggct catgccgggt ccttaccccc ggtcgtgtgg
     2461 caatttctcc aaacggttcc agcttctgcc aatgtcatgg atgtgctgcg aaccggggtg
     2521 tcgtttctcg ggatgatcga tcctgaagct ccggatatct cccatgaggc aaacctccga
     2581 aaggccgtcc gtttactcgc aaaaatgcct ctacttcttt cctgggccgt tcgaacacga
     2641 ttcgatcatc ctctatttca ggctccggcc acaaagtcgt ttgcccaaag cctcttgtat
     2701 ctgttgacag ggagtctccg ggaagatcat gcgaaagaac tggaggtt
//
LOCUS       DATIAL010000580         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1285386, whole genome shotgun sequence.
ACCESSION   DATIAL010000580 DATIAL010000000
VERSION     DATIAL010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285386"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1806
                     /locus_tag="VLA60_17580"
     CDS             340..1806
                     /locus_tag="VLA60_17580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF11227.1"
                     /translation="MPENSSIVIMGSPDLEKALSREGLGVVRLETPENVKSLEIGSQT
                     RLVILDGQYLGDSLVFDMMTTIQEHRPYLDVLVFKPGADAAYVHSVLKNGASDVIYED
                     SHEKLHQAIEAIQTNQKFSPDTPDLRDTRKRLGSFEGILSRNGVMWDIFETITRTASS
                     DATVLIVGETGTGKDLLARAIHRQSGRTGRFVAVNCSTISPEIIESELFGHERGAFTG
                     AHQQKQGLFRYADGGTILLDEIGDMPVQTQLSLLRVLQEQTVRPVGSHHEVPIDARVI
                     AATNASLANAVQTGSFREDLFYRLDVIRLEVPPLRERREDILYMFGHFLKRVCADYGM
                     DCPELHQSFMDLLLEQSWPGNVRQLENLTQRLVLKGHTRELTAEDLAEVLYSQDIPGS
                     SSALPAPEAESVDQPVPSQGDHTPSINPSLTLAAYLEPHLTTLEKEYLETLLSENSGR
                     IAESANQAGISRRTLLRKLKTYRINKQYFRNKNSASKE"
     gene            complement(1976..3316)
                     /locus_tag="VLA60_17585"
                     /pseudo
     CDS             complement(1976..3316)
                     /locus_tag="VLA60_17585"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3407..>4158)
                     /locus_tag="VLA60_17590"
     CDS             complement(3407..>4158)
                     /locus_tag="VLA60_17590"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF11228.1"
                     /translation="RPPDYAVPLDELWSAINPRTRAIILNTPHNPSGHVFTRDELNAI
                     LTLADQHGIYVVTDEIYDHLYYEPYRHIAPATLAPDWKGLVVTGGLSKIYAATGWRLG
                     YVLASKDVTTAIRTVHDYLTICAPTPFQYAAVTALALPETYYTDLRARFLRRRDLTMQ
                     MLTDSGFEPYVPQGAYYLLAGYGPWEHQGDSQSFATRLITEAKVAVVPGSAFYYQATD
                     LGQHLVRFAFAKTSQVLKQAGENLAKAFHKR"
BASE COUNT         1011 a         1118 c         1050 g          979 t
ORIGIN      
        1 taatttacga gggcgaatgc gaaggtactg cataccgtat tcccattgcc ccggcacagg
       61 ttctcaggcg caaccagaca aaatggtctt ggcccgatca ccgaattgaa tttaaaccag
      121 ccccttcaca gacagtcggc ccgtttattt tttggtaatt ttttatggca atcgcgaaac
      181 ttcctcgatc catacctcaa tatacctata catctcgcaa gacctgttcc atcagttaac
      241 ccgttgatat ttgtgatttt gtggaatttt gggactgtta tgtccctata tttgggacag
      301 acttgactca tttaaatatt atgagacact attgtctcaa tgcctgaaaa ttcttccata
      361 gtaatcatgg gttccccgga tttggaaaag gccttaagtc gtgaaggcct tggagttgta
      421 cggctggaaa ccccggaaaa tgtgaaatca ttggagattg gttctcagac acgcctggtc
      481 atactcgatg gccagtacct tggagactcc ctggtgtttg acatgatgac gaccatacaa
      541 gaacacagac cctatcttga tgtcttggtg tttaaaccgg gagccgatgc agcctatgtc
      601 cattcggttt tgaaaaacgg ggcatcggat gtgatttatg aggattcaca tgaaaaactg
      661 catcaagcca tagaggccat tcagaccaat caaaagtttt ctccggatac accggatttg
      721 cgtgataccc gcaaaagact gggaagcttt gaaggcattc tcagccgtaa cggagtcatg
      781 tgggatattt ttgaaaccat cacccgcacc gcaagttctg acgccaccgt attaattgtg
      841 ggggaaaccg gaacgggtaa agacttgctg gctcgggcga ttcaccggca gtccgggcga
      901 accgggcgat ttgtggccgt caattgcagc acgatcagtc cggaaattat tgaatcggaa
      961 ctgttcggcc atgaacgcgg ggccttcacc ggagcccatc aacaaaagca gggattattt
     1021 cggtatgccg atggcggcac catccttctg gatgagattg gggatatgcc ggttcaaacc
     1081 caactcagtc ttttgcgggt attgcaagaa cagacggtga gaccggttgg atctcatcat
     1141 gaagtgccaa ttgatgcgcg tgtcattgcc gcaacaaatg cctcattggc caacgccgtc
     1201 caaaccggct cctttcgcga agacctcttt taccggttgg atgtcatccg gttagaagtg
     1261 ccccccttac gagagagacg ggaggatatc ctctatatgt tcgggcattt tctgaaacgc
     1321 gtgtgtgcag actatggaat ggactgtccg gaattacacc aatccttcat ggatctactc
     1381 ctagagcagt cctggccagg caatgtgcgg caactggaaa acctcacgca acgcctggtg
     1441 ttaaaagggc acacccggga attgactgcg gaagacctgg ccgaagtatt gtattcccag
     1501 gatatcccag gatcctcctc ggctcttcca gctcctgaag ctgaaagcgt ggatcagcct
     1561 gtcccaagcc aaggtgatca cactccctcc atcaatccgt ccctcacgct ggccgcctac
     1621 ctcgagccac acctcactac tcttgagaag gagtatttgg aaaccctgct gtctgaaaat
     1681 agtggtcgca ttgccgagtc ggccaatcag gccgggataa gtcgacgaac actcctccgt
     1741 aagctcaaga cctaccgcat caacaaacaa tattttcgaa acaagaattc agcatcaaaa
     1801 gaataatcct gttccctagg atggatgagg ggaaaagaca ttcaaggatt gacgtaccgc
     1861 tcacggagat agctcaacaa atctttaaca tggtcacggg ttcccacggc ctttcgtaag
     1921 aggtccggcg tctcatgtag gtgcccatgg cgatggatcc gttcccgcaa aaaattcaga
     1981 atggtcgcaa aatctccctc ttcgatgcgg cactccagat ccgtgaagta ttcgcgtaag
     2041 acggcaaaga gcggagccgc atagagattt cccagagtat aactcggaaa tacccaaagg
     2101 ccccacccgc ccagtgggca tcctgcaata caccttctgc atcatctttt ggtcgcactc
     2161 ccagaaattg ttcactcaat cgggtccatt cggctgggac atcctcaacc gtgatgcgtc
     2221 gctccaacag agcagcctcc atttcaaacc gcaaaatgat aagcaggtta taggaaactt
     2281 cctccgcaaa aatccgaatg ctttccgacg tcacccggtt ggaggagagg aagaccaatt
     2341 ccggatcaaa ggcaatgtca ggaaaatggc gagcgaacac cggccgcagc caccgataaa
     2401 acccacggga tcgtccaatc atgttttccc acaggcggga ttgggattca tgcatgccgg
     2461 ttgacgtcgc cgcatacacg gctgatccac caggtccctc cgggatacct tgctcgttca
     2521 gggcatgccc gagttcatgc atggtggccg ccaacccgct gagcagatcc ccttcgtgga
     2581 catgtgtggc gatacgcaca tctccgctgc ccacccggca tgaaaatgga tgctccgcct
     2641 cgtgcaacgc tcctcgtgtc gtatcgtatc ccaccgcctt aatgacatcc tggtgcatcg
     2701 ccaattgtgt ggccttatcg aaataatcgt tgaaggcaac cgatcctttt ttgtccagag
     2761 cctggcgccg gatatcagtc aaaccttgtt gcagcgcgcc aaacatccgg cgtaaatctt
     2821 caacggtcgt gccgggatca tagtcctcta gaaggacacc atagggaggc cggttggagt
     2881 caatggccta tgcccgctcg agtgccaaat ccacattcgt ctgtaggctc tctgcaaaaa
     2941 gacgatagtt tttttctttt ttcgcttcgc cccatgcggc gacaccactg gcgctggcct
     3001 tcgcgatcct gctggcgagt ttcgacggca cacaagtcgc ccggcggtag gttcggcgca
     3061 tatttcgaac cgccgcttgt tgctccggag tcgaatcagg tagggcagcc accgcctcga
     3121 tgagttcacc cagtcgaggt gatgtggctt gttcatgata caaccgaccg attaactcac
     3181 tttgttgagc acggccccct gccgaccctg aagacatggt cacttgctga tcccaaccca
     3241 gtagcccgcc aatgcctgcc agggtatcca gttcggcaat ctgctgagac agttcatccc
     3301 atactgatat ggtcatacac tattcccttc tctaaactag tcagttggtt tggagttgcg
     3361 agggttttca tgagagggtc gtgaagacgt tccggtagtt tctccctcaa cgtttgtgaa
     3421 acgcctttgc gagattctca ccggcttgtt tgaggacctg gctcgttttg gcaaaggcaa
     3481 aacggacgag gtgttgtccc agatctgtcg cctggtaata aaacgcgctg cctggaacca
     3541 ctgccacctt cgcttcggtg atcaaccggg tggcaaacga ttgggaatca ccctgatgtt
     3601 cccaaggccc atagccggct aaaaggtaat aggccccttg aggtacatag ggttcaaacc
     3661 cactgtcggt cagcatctgc atagtgagat cccgacgccg aaggaatctc gctcgtaagt
     3721 cggtgtagta agtctcgggt aaggccaaag ccgtgactgc cgcatattga aagggcgtgg
     3781 gcgcacagat cgtgaggtaa tcatgtaccg tgcggattgc cgtcgtcaca tccttagaag
     3841 ccagaacata cccaagacgc caccctgttg ccgcgtagat ttttgacagg cctccggtga
     3901 cgaccagacc tttccagtcg ggcgccagcg ttgccggggc aatatgccga tagggttcgt
     3961 agtacaaatg atcataaatt tcatccgtta ccacatagat cccatgctga tcggccagag
     4021 tcaggatggc gttcagttca tcccgcgtga acacatggcc ggatgggtta tggggagtat
     4081 tcagaataat ggccctggtg cgagggttga tggctgacca aagttcatcc agaggtaccg
     4141 cgtaatcagg tgggcgaa
//
LOCUS       DATIAL010000581         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_210816, whole genome shotgun sequence.
ACCESSION   DATIAL010000581 DATIAL010000000
VERSION     DATIAL010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210816"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VLA60_17595"
     CDS             <1..1621
                     /locus_tag="VLA60_17595"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HSF11229.1"
                     /translation="SAHDVHQALLRDPRSHSLVQPLSFYHDVQGNMHLSTRVTGPLTH
                     PSQWHILDGDLLLDEVHLSTSEDRLPLNHIKGHLSFNQEQIHIHRINGMLGKSPVDIK
                     GQWSLRKDSKASNLHLGGMWSSIDLQSLFPSVVQTFSTFEGLVGTTLTLSGSNLRPEY
                     HAELDFTNIVLTTKGLFHKPSGVPAVVQAKGTIKKEKAIRMTKGTLSIPPYTLEAQGQ
                     LSWSDRPHVRGFLQTESGTGAMFPPGVVIGEKNLGLSSLGITWGLEGKNWDWTTWSMK
                     GKIEGTNRNSESTTSNTNKEVKSASFQWAQKNQRGKGELTLKDIPIESLLVPQSDTPS
                     PLTGTASLVTSLHMSLGSRELIQRSLTGKGHAQLQKGLIQTGPVLSKILGILNVPSLL
                     MGKVNLLEEGLPYDQIEGSFAIENGLLSSQDLALKSPVIKLTAAGTYDLPTEKLNGMV
                     AVSPFGAYSRLLKDIPLFGSLMKGDRKGLMTALFEVKGPRTEPDITYLPLESFAGGLK
                     GLAQFAVDVLKNVVTLPIPDTESQNAERTSK"
     gene            1704..>2289
                     /locus_tag="VLA60_17600"
     CDS             1704..>2289
                     /locus_tag="VLA60_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11230.1"
                     /translation="MNKKHHRLSFRFSSIFWILPVLLTGCFSPKALEHVVIAYDHSVT
                     RSLVEQLLLNIARSHHHQPVHFTAISSIAATFNYQFSAGATPPLGGLDGGFALAPVFG
                     GSIAENPTFTISPIEGEDFTQRLLTPLREGKLTLLLRQGVDIDLLLRLMAGEIRTTMN
                     NQDTAYYNRPADQIGYPKFRQIVLHLSRLQDNNQL"
BASE COUNT          602 a          618 c          521 g          548 t
ORIGIN      
        1 gtccgctcat gatgtgcacc aagccctgct ccgcgaccct cggagtcact ctcttgtcca
       61 accattgtcc ttctatcatg acgttcaagg aaacatgcac ctctccacac gggtaaccgg
      121 tcctctgact cacccatctc aatggcatat tcttgatggc gacctcctgc ttgacgaagt
      181 gcatctttcc acctccgaag acaggcttcc cctcaatcac atcaagggcc atctgtcgtt
      241 taaccaggaa cagattcata ttcaccggat caacggaatg cttgggaaaa gcccggtaga
      301 catcaagggc caatggtcgc ttcgcaaaga ttcaaaagct tcaaaccttc acttgggcgg
      361 tatgtggtct tcaattgacc ttcagtccct ctttccttca gtcgtccaaa ccttttccac
      421 ctttgaaggt ctggtcggaa ccaccctcac cttgtcgggc tcgaaccttc gccctgagta
      481 tcatgcagag ttggatttca ccaatatcgt tcttaccacc aagggtctgt ttcacaaacc
      541 atctggagtt cctgcagttg ttcaagccaa aggaaccatc aaaaaggaga aggcaatcag
      601 gatgacaaag ggcacattgt ccattcctcc ctataccctc gaagcacaag gccaattatc
      661 gtggtctgat cgaccccatg ttcgtgggtt ccttcaaacc gaaagtggaa cgggagccat
      721 gtttccgcca ggcgtcgtca tcggtgaaaa aaatctgggt ctctccagtt taggcatcac
      781 atggggtctt gagggcaaga attgggattg gacgacttgg tctatgaaag gaaaaatcga
      841 agggaccaac cgaaactcgg aatcgacaac atccaacaca aacaaagaag ttaaatcggc
      901 ctcattccaa tgggcgcaaa agaatcaaag agggaaggga gagctcaccc tcaaagacat
      961 tcccatcgaa agcctcttgg tacctcagtc cgatactcca tctcccttga caggaacggc
     1021 gtctttggta acctcgctac acatgagtct tggaagccgg gagctgattc agcgctcgct
     1081 gacggggaaa ggacatgcgc aactccagaa gggactgata cagaccggtc cggtcctgtc
     1141 aaaaattctg ggcattttga atgtgcccag tttattaatg ggaaaagtca acctgctgga
     1201 agagggactc ccctacgatc aaatagaagg atcgtttgcc atagagaatg ggctactgag
     1261 ttcccaagat ttggcactga aaagtccggt catcaagtta accgccgcag gcacctatga
     1321 ccttcccacc gaaaagctca atggcatggt agccgtcagc ccgtttgggg cctattcgcg
     1381 tttactcaag gacatccctc tgtttggctc tttaatgaag ggtgaccgaa agggccttat
     1441 gacagcgttg tttgaagtca aaggaccccg aaccgaaccg gacattactt acttaccgtt
     1501 ggaatccttc gccggagggc tgaaagggtt agcgcaattt gcggtcgatg tcctcaaaaa
     1561 tgtcgtcacg cttcctatcc cggataccga aagccagaat gcagaacgca cttccaagta
     1621 acacatcggc agagaagccc ataagccagt ttagggataa atttcagtag gagaaacagc
     1681 aatgactaag ggagaaggac aagttgaata aaaaacatca tcgtctatct tttaggtttt
     1741 catccatttt ctggatcctg cctgttcttt tgaccggctg tttttccccg aaagctctgg
     1801 aacatgtggt cattgcgtat gaccattcgg taaccagatc cctggtcgaa caattgctcc
     1861 tcaatattgc cagatcccat catcatcaac ccgtgcattt cacagccatt tccagcattg
     1921 ccgccacctt caattatcaa ttttcggccg gggctacccc accgttaggg gggttagatg
     1981 gcggatttgc attggcgccc gtctttggag gcagcatcgc tgagaacccc acgtttacca
     2041 ttagtcccat tgaaggagaa gatttcaccc aacgcctgct gacccctctc cgggaaggaa
     2101 aattaacgct cctgctgaga cagggggtcg atattgattt gctccttcgg ctcatggccg
     2161 gggaaatccg tacgaccatg aacaatcaag ataccgctta ctataatcga ccagcggatc
     2221 agatcggata tcccaaattt cgacaaatcg tccttcatct ttcccgttta caggacaaca
     2281 atcagctct
//
LOCUS       DATIAL010000582         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_640926, whole genome shotgun sequence.
ACCESSION   DATIAL010000582 DATIAL010000000
VERSION     DATIAL010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640926"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VLA60_17605"
     CDS             <1..222
                     /locus_tag="VLA60_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11231.1"
                     /translation="AVYQLKDPNYSAWVPANEWIRPIPSCFKFLMYFDAESPAWDPIP
                     WLLGIDVLFGIGSSVTQIGGTVYKYQHSS"
     gene            588..1136
                     /gene="trmL"
                     /locus_tag="VLA60_17610"
     CDS             588..1136
                     /gene="trmL"
                     /locus_tag="VLA60_17610"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HSF11232.1"
                     /translation="MKPVRLTVCLLGIYDIKGSLISGFLIPTMLDVVLYEPEIPPNTG
                     NIIRLCANTGFGLHLIHPLGFELDDKRARRAGLDYHELARMKDYPTLQDYLDAQKPAR
                     IFAITTKGQRPYHDVAFQPGDALYFGPETRGLPAHILEALPPTHRLRIPMRPDSRSLN
                     LSNAVAVVVYEAWRQLQFHGAK"
     gene            1356..2714
                     /locus_tag="VLA60_17615"
     CDS             1356..2714
                     /locus_tag="VLA60_17615"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HSF11233.1"
                     /translation="MKQILFVGAGSVGGYFGAHLARHNPNVSFLLRPRTREAVAKNGL
                     SIRSAIGESFTVHPTVASDPEELPQPDLIVLGIKAYDLDAVMNQIEPVLKPDTVILTL
                     QNGVTIEDTLMVRFGRERIVGGVAFIYSKIAEPGVIDHYKKGMLTIGEPLGMETPRLQ
                     AILALLKGAGIPCFINPDIRRAKWEKMCWNCVFNPLTVLLNDRVAKALDHPEFRPVIA
                     TIVREVSAVAMAAHRVPLDEDMPEKVVKWAQELRDIHTSMYDDWKAGRPTEIDELNGF
                     ITEQGKAFGVPTPMNDMLTALIKALTGPEPSAEPTIQIEGAIMQSLHFTRTTLTELPT
                     THQVFELPTLLPGMNVRGVKLQALLDVVTLNVGADHVTFESQDGKFSACLTIKQAADF
                     GILVYEQDGAPFPSERGGPFRLVTPGLGDLCANVKQVGKIIFSKGLISDSRPPQVCPE
                     KA"
BASE COUNT          633 a          660 c          784 g          698 t
ORIGIN      
        1 gccgtctatc agctaaaaga cccgaactat agcgcctggg tgccggcgaa tgaatggata
       61 cggccgatac ccagttgttt caagttcctg atgtactttg atgcggaatc gcccgcttgg
      121 gatcccatcc cctggttgct cggcatcgat gtcctcttcg gaatcggatc gagcgtcacg
      181 caaatcggcg ggacagtgta taaataccag cattcgagtt aggggatgga gaatcaggaa
      241 tgattaacag gggagattcc tggctccacc gaggtcggaa aagcttcctc cccctccttc
      301 attgggcctt aaaatcatat gccgtcttac cgacgtgact ggagatgtgt taccaatcga
      361 aagcactggg gccagtatgc ggggcagtga gtggcagatg actcaccgcg aggtgcacgg
      421 gaactccgcc attttcttca gcaatgaatg ggtgttccca gggaaggccc acactaaacg
      481 caagccatgt gttctgaaat gggtatgcga aggggctcgg ctgcagccag gttttcttcg
      541 ttcccccaac gaacaacggc tttctcctaa gatggtagta attatagatg aagcctgttc
      601 gcctgacagt ctgtctttta ggaatatatg atattaaggg aagtctcata tccggtttct
      661 taatccctac gatgttggat gttgtcctct acgaaccgga gattccgccg aacaccggga
      721 atattattcg tctctgtgcg aataccggat tcggtttgca tctgattcat ccgttgggat
      781 ttgaattgga tgataagcgg gcccgccgtg ccggattgga ttatcatgaa ttggcccgga
      841 tgaaagatta tcccacgctg caggactatc tggatgctca aaagccggcg cgaatttttg
      901 cgatcactac aaaaggacag cgaccctatc acgacgtggc ttttcagcca ggcgatgcct
      961 tgtactttgg tcccgagacg cggggcttgc ctgcccatat tttagaagcg cttcctccca
     1021 cgcatcgttt gcgaattccc atgaggcccg attctcgtag tttaaacttg agcaatgctg
     1081 ttgccgtcgt cgtctatgag gcttggcggc agctgcagtt tcatggtgcc aagtaagaca
     1141 tagatccgta ggcgagcggt ggtaattctg ataaagtgag ggattctgtt ttggcataaa
     1201 tacattggat tccgagcctg ttgctttggg cacagatcct tcgcaaaagc tcaggatgac
     1261 agcaaggttc agggctcatc atgatttggc ttgttgatat ttaaaccatt acgcgaaatt
     1321 ttagtaaaaa tggaaaggta atcaaaggta agcagatgaa gcagattttg tttgtggggg
     1381 cagggtcagt tgggggatat tttggggcgc atctggctcg ccacaatcct aatgtgtcgt
     1441 tcctcctgcg gccgcgaacc agggaggccg tggctaaaaa cgggctgagc atccggagcg
     1501 cgattggtga gagttttacc gtgcatccca ccgtagcttc cgatcccgag gaactgccac
     1561 aaccggattt gattgtgctc gggatcaaag cctatgacct ggatgcggtc atgaaccaga
     1621 ttgagccggt actgaaaccg gataccgtta ttctgacctt gcaaaatggc gtaacgattg
     1681 aggataccct gatggtccgc tttggccggg agcggattgt gggcggggtg gcatttatct
     1741 attccaagat cgccgagccg ggggtgatcg accattataa gaaaggcatg ctgactattg
     1801 gggaaccgtt ggggatggaa actcccagac tccaagccat cctggcgtta ttgaaagggg
     1861 cagggattcc ctgtttcatt aatcctgata tccggcgggc caagtgggag aaaatgtgtt
     1921 ggaattgtgt gtttaacccg ttgacggtgt tgctgaatga tcgggtggca aaagccctcg
     1981 atcatccgga gttccgaccg gtcatcgcga ccatcgtgcg ggaagtctcg gccgtggcca
     2041 tggcggcgca ccgtgtgccg ttggatgagg atatgccgga gaaggtggtc aagtgggctc
     2101 aagaacttcg agatattcat acgtcgatgt atgatgattg gaaggccgga cggcccactg
     2161 agattgatga gctgaatggt tttatcaccg aacaaggcaa ggcgtttgga gtgcccactc
     2221 ctatgaacga catgttgacg gcgttgatta aggccctgac tgggccagag ccttcagccg
     2281 agcccacgat acagatagag ggggccatca tgcagtcgct ccattttaca cgaacaacgc
     2341 tcacggaact tcctaccacg catcaggtgt ttgagctgcc cacgttgctg ccgggcatga
     2401 atgtgcgtgg cgtcaaactt caagcactac ttgatgttgt gacgctcaat gtgggagcag
     2461 atcatgtgac cttcgagtcg caggatggaa aattctcggc ctgtttaacc atcaagcaag
     2521 ccgcagactt tggcattctg gtgtatgaac aggatggtgc gccgttccct tccgaacgag
     2581 gcgggccgtt tcgattggtc actcccggat tgggtgatct gtgcgcaaat gtgaagcagg
     2641 ttggcaaaat catcttttcc aaaggcttga tttcggatag ccgtccacct caggtctgtc
     2701 cggagaaagc gtaggtttgt cttgatgcac ccttctcacc gcgttcctct tatctataat
     2761 ttgttccctc gcctg
//
LOCUS       DATIAL010000583         3951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1285941, whole genome shotgun sequence.
ACCESSION   DATIAL010000583 DATIAL010000000
VERSION     DATIAL010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285941"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VLA60_17620"
     CDS             complement(<1..496)
                     /locus_tag="VLA60_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11234.1"
                     /translation="MLNSGPNIKPRRTPSPSSTETLSPGSSHPLHIEKLVAGGFGLGR
                     VDGQVVLCAGGIPGEALQVEILSRRKGVSQGKILQVQDPAEGRVVPLCPVVGRCGGCQ
                     LQHIQYEEQLSQKRLILEDTLRRIGKISDVLIAPVIPSPRPYGYRQVLRMGIGEGPDG
                     LFLGF"
     gene            complement(471..1844)
                     /locus_tag="VLA60_17625"
     CDS             complement(471..1844)
                     /locus_tag="VLA60_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HSF11235.1"
                     /translation="MAETICLIDDEPAILNTLSSILEDEGYQVLVAKSGLEALKLVRS
                     EVPDLVILDIWMPEMDGLETLKRLRTQFPNILVVMMSGHGSIETAVKSTKLGAYDYLE
                     KPLDLEKVTILVRNALHQRKLEEENLNLRIQVERRFELVGSSPPMERLRELIAMAAPA
                     NSRVLIAGANGTGKELVARAIHLQSPRHNRSFVEINCAAIPETLIESELFGHEKGAFS
                     GASSMKRGKFELADGGTLFLDEIGDMSLATQAKVLRALQEQQFTRVGGTKLLNVQVRV
                     IAASNKDLAEEIGKGTFREDLYYRLNVLPIVVPTLKERRDDIPELAQHFLRLHSEEQG
                     MKPKEFSPQGMEALQRHDWPGNIRELRNLIERLLIMVPKTIIDAADVEMFLQGRAPSG
                     VPSLAVGTNYDSLREARNAFEREVISLKLRENNWNVSKTADELKIERSHLHRKIKLLH
                     VELRPEH"
     gene            complement(1947..>3951)
                     /locus_tag="VLA60_17630"
     CDS             complement(1947..>3951)
                     /locus_tag="VLA60_17630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF11236.1"
                     /translation="NLIRAFFEKRHKLLGKGFRSKLVAAFIGFALIPTVLLALVASGV
                     ISEVIDVWFNDQIMQVLRDSEEVAHLYQEERESLTADTARAISKEIFREDILKPEHRE
                     LLRAIMARMQQEYHLAGVEVFSPKMETLARMMDPKLSDAVLSLPVGQLVLHVLDTGQP
                     MSSAQEAPVGKLIRAAAPIRGNGQTDRVFGVVVVSSYVPEVLVMKMDGIAKQFEEYRQ
                     IISMKNPIKGGAYLFVAVVTVLILFGATWFGFYVARGITVPIQRLAEGTEAVAKGNLD
                     VNIDVKATDEIGTLVQSFNRMTADLRTSKSSLEEVNMSLVRSNVEIDQRRAYTEAVVE
                     TIAAGVLSIDMAGLISTFNPSAERIIGVKGDDLRGRPVHDAFKAFNLQLFQKAYDQML
                     QEQTESVSMDGQMETPNGRLLTVGLNISRMQNDAGLDLGFVFVFEDRTEFIKAQKTAA
                     WQEVAQRIAHEIKNPLTPIQLSAQRLRKKYAEKSTGFDEIFDQATGVIISEVTRLKGM
                     VDEFSKYARMPLPQMRKASLHDIIEKVISLYTGAHRDIEFIVKFDEDLPALNCDPEQM
                     HRVLVNLFDNAIQAMNDHGRLWVTTECDWRRHRAVVRVADEGMGIQPDDHKMLFVPYF
                     SKKRTGTGLGLAIVHRIISDHNGTIHAENHHPKGALFTFELPL"
BASE COUNT          962 a         1123 c          899 g          967 t
ORIGIN      
        1 aaaaccccag aaataatccg tcaggcccct ctccgattcc cattcgaagg acttgccgat
       61 agccataggg tcttggagac gggatcactg gagcaatcag cacatcggaa atttttccaa
      121 tccgacggag ggtatcttcc agaatcaatc gcttttgaga aagttgctct tcgtattgaa
      181 tatgctgaag ctggcatcct ccacatcgcc cgacgaccgg gcagagggga acaacccggc
      241 cctcagccgg atcctggacc tggaggattt tcccttggct cacgcctttg cgccgactga
      301 gaatctccac ctgaagggct tcaccgggaa tgcctccggc acacaacacc acctgaccat
      361 ccactcgtcc gagtccaaat ccgccggcca ccaatttttc aatgtggagg ggatgggaag
      421 atccgggaga caacgtttca gtagaagagg gagaaggagt cctgcgaggc ttaatgttcg
      481 ggccggagtt caacatgaag gagtttaatc tttctgtgta aatggctacg ctcgattttc
      541 aattcatcag cggttttgga aacattccaa ttattttccc tgagtttaag agaaatgact
      601 tcccgctcaa aggcattccg ggcttcccgg agtgagtcgt agttggtgcc gacggcaagg
      661 gagggcaccc cgcttggcgc ccgaccctga aggaacatct caacatctgc cgcatcaatg
      721 attgtcttcg gcaccatgat taataagcgt tcaatcaaat tccggagttc acgaatattg
      781 cccggccaat catgccgctg caacgcctcc atgccttgcg gactaaactc tttaggtttc
      841 atgccctgct cttcagaatg cagtcgcaaa aaatgctgcg cgagttcagg gatatcgtcc
      901 cgacgctcct tgagtgtcgg aaccacaatc ggcaaaacat ttaaccggta atacagatct
      961 tcacggaacg tccctttccc gatctcttcc gccagatctt tattggatgc ggcaatgacc
     1021 cggacctgga cattaaggag tttggttcca ccaacccgtg taaattgttg ctcttgcagg
     1081 gcacgcaaca ctttggcttg tgtggccaga ctcatgtccc cgatttcatc taaaaacagg
     1141 gtgcctccgt ccgccagctc gaacttgcca cgtttcatag agctggcgcc actgaaggcc
     1201 cctttttcat gcccaaataa ctcactctcg attaacgttt ccggaatcgc cgcacaattg
     1261 atttccacaa aggaacgatt atgccggggg ctctgcaaat ggatcgcgcg ggcgaccagt
     1321 tctttccctg tcccatttgc cccggcaatc agcacacgac tattggctgg agccgccatc
     1381 gcgatcagtt cgcgaagccg ctccataggg ggagaagaac ccaccaattc aaatcgtcgc
     1441 tccacctgaa ttctgagatt caggttttcc tcttccagct tgcgttgatg caacgcattg
     1501 cgcactaata tggtgacttt ttctaagtct aagggctttt ccaaataatc ataggcgccc
     1561 aattttgtgg atttgacggc ggtttcaatg gatccatgcc cggacatcat cacgaccagg
     1621 atgttgggaa actgggtgcg caatcgtttt aaggtctcca gcccatccat ctccggcatc
     1681 cagatatcca aaatgactaa atccggcacc tcacttcgga caagcttgag cgcctcaaga
     1741 ccgctcttgg ccaccaacac ttgataacct tcatcctcaa gaatgctact taaggtattg
     1801 agaatggcag gttcgtcatc aatcaggcaa atggtctcgg ccacaatact ccctccatca
     1861 ggtgtggaat gaatggtctc tcacgtgtgt cacgcgagac aacccttccg gagaaaaggc
     1921 cgtccagcca ctgacgctct cctctcctac aacggcaatt cgaatgtaaa taacgcacct
     1981 tttggatgat gattctccgc atgaatggtc ccattgtgat cggagattat acgatgcaca
     2041 atggctaatc ccaaccctgt tcccgttcgc ttttttgaaa aatacggcac gaacaacatt
     2101 ttgtgatcat ccggctgaat acccatccct tcatccgcca ccctgaccac ggcccgatgc
     2161 cggcgccaat cgcactccgt cgtcacccag agacggccgt gatcgttcat ggcctggatc
     2221 gcgttatcaa ataaattgac cagcacccgg tgcatctgct ccggatcaca attcaaggcc
     2281 ggaaggtctt catcaaattt gacaataaat tcaatgtcac gatgtgcccc cgtatagagg
     2341 gagatcactt tctcgataat gtcatgcagg gatgctttcc gcatctgggg aagcggcatt
     2401 ctggcatatt tcgaaaactc atcgaccatc ccttttaacc gggtgacttc gctaataatg
     2461 acgccggtgg cctgatcgaa tatttcatca aatcctgtcg acttttccgc gtattttttt
     2521 cgcaaccgtt gggccgacaa ttgaatcggg gttaagggat tcttaatttc gtgagcaatg
     2581 cgttgcgcca cttcctgcca ggccgccgtt ttttgcgcct tgatgaattc cgtccgatct
     2641 tcaaacacaa acacaaaacc caggtccaaa ccggcatcgt tctgcattct ggaaatattg
     2701 aggcctacgg tcaagagccg gccattgggc gtttccatct ggccatccat ggacacactt
     2761 tccgtctgtt cctggagcat ctggtcatag gctttctgaa acaattgcag attgaaggcc
     2821 ttgaacgcgt catgaaccgg gcgtccacgc aaatcgtctc ccttcactcc aataatccgt
     2881 tcagcagagg ggttaaaggt ggaaatcaga ccggccatat cgatcgataa gactccggcg
     2941 gcaatggttt ccaccaccgc ttcggtgtag gcccggcgtt gatcgatttc cacattggat
     3001 cgtacgagcg acatattcac ttcttccaag ctggatttac tggtgcgcag atcggccgtc
     3061 atgcgattaa acgactggac caaggttccg atttcatccg tggccttgac gtcgatattc
     3121 acatctaaat tacctttggc taccgcttca gttccttcag ccaatcgttg aatgggaacc
     3181 gtaatccctc gggccacata aaatccgaac caggtggcgc caaataaaat cagcaccgtt
     3241 accacggcca caaacaaata ggctccaccc ttgatcggat ttttcatgga gatgatctgc
     3301 cggtactcct caaattgttt ggcaatccca tccattttca tcaccaggac ttccggcaca
     3361 taggacgaca ccaccaccac gccaaatacc cggtcagttt gaccgtttcc ccggatcggc
     3421 gccgctgccc gaataagttt ccccacggga gcttcctggg ccgaagacat gggctgaccc
     3481 gtatccaaga catggaggac caattgcccc acaggaaggc ttaacaccgc atcagacaac
     3541 ttaggatcca tcatgcgggc taaggtttcc atttttggtg aaaagacctc cacacccgct
     3601 aaatgatatt cctgttgcat gcgggccatg atggctctca ataattcccg atgttcgggt
     3661 tttaatatat cttcccgaaa aatttccttg ctaatggcgc gcgccgtatc cgctgttaat
     3721 gattcacgtt cctcttgata gagatgggcg acttcttccg aatcccgcaa gacctgcata
     3781 atctgatcat taaaccacac gtcgatgact tcactgatga ccccgctggc caccaaggcc
     3841 agcagcaccg tgggtatcag ggcaaagccg ataaaggctg cgaccaactt tgaccggaag
     3901 cccttcccca acagcttgtg acgcttttcg aaaaaggccc gaattaaatt t
//
LOCUS       DATIAL010000584         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_748358, whole genome shotgun sequence.
ACCESSION   DATIAL010000584 DATIAL010000000
VERSION     DATIAL010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748358"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2304)
                     /locus_tag="VLA60_17635"
     CDS             complement(<1..2304)
                     /locus_tag="VLA60_17635"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HSF11237.1"
                     /translation="MSPPSLDKTYNAAEIEKRWGEFWIEHHFFQPDPMDNSPTFCLVM
                     PPPNVTGSLHIGHALNTTLQDIIVRWRRMQGFKTLWIPGTDHAGIATQNVVERQLAQE
                     NQSREHLGRQAFIERIWKWRSQSGGIIIGQLKQLGASCDWTRLRFTMDEGLSLAVREV
                     FVSLYEEGLIYRGERLINWCPRCLTALSDIEVEHESVKGKLYHIRYFLTDDSMASLVV
                     ATTRPETLLGDTAVAVHPEDRRYRHYLGKKVRVPLTTRTIPIVADPILVDREFGTGAV
                     KITPAHDFNDFEAGERHRLPRLAIFDFQARMSPLGLQEARVDTELRSALNLTPVPEAR
                     ALVIDALKKHDLLVGEEDHALAIGKCYRCKTVVEPYLSPQWFVNIQPLAIPAIQVVET
                     GQIRIIPEGWKNNYLGWMQQIKDWCISRQIWWGHQIPAWYCRTCNKDNLQEAGHVVGA
                     TKEYGIPEPSLRSCFIPSDANPIVSRTPPQSCPTCGGTDLFQDPDVLDTWFSSALWPF
                     STLGWPEKTEDFKAFYPTATLVTGQDILFFWVARMIMMGLKFTGNVPFRNVYIHALVR
                     DAEGKKMSKSKGNVVDPLTQMRQYGTDALRFTLASMSSPGRDIKLAEERIEGNRNFVT
                     KLWNAARFIQLYADGPRASLVPQSRPFPDKWILSRLNHTVREVTKAFEDYRFDQAASA
                     LYQFIWHEYCDWFIELAKPCLQQENHSDAPATRQTLLESFEVIQRLLHPVMPFVTEEI
                     WQSFPHSGQSVMTQPFPTEKLEWADAEA"
BASE COUNT          643 a          561 c          637 g          625 t
ORIGIN      
        1 agcttcggcg tcggcccact ccagtttttc agtgggaaag ggttgagtca tgacggactg
       61 cccgctgtgg ggaaaagatt gccatatttc ttcagtaaca aagggcatca cggggtgtaa
      121 cagccgttgg atgacttcga atgattccaa gagtgtctgg cgggttgcag gtgcatcaga
      181 atggttctcc tgttggaggc agggtttggc gagttctata aaccaatcgc agtattcatg
      241 ccagataaat tgatagagag cggaggcagc ttggtcaaat cgataatctt caaacgcttt
      301 ggtcacttca cggacggtgt ggttgaggcg gctgaggatc catttgtcag gaaaaggtcg
      361 agactgtggt acaagcgatg cccgtggtcc atccgcatag agttgaataa atcgtgcagc
      421 attccagagt ttcgtaacaa agttccgatt cccttcaatg cgttcttcag ccaatttaat
      481 gtctcgtccc ggtgaggaca tagacgccag agtaaatcgt agagcatcag ttccgtattg
      541 gcgcatttgg gtcaggggat caaccacatt tccttttgat ttgctcattt ttttgccttc
      601 ggcgtcacgg accaaggcgt ggatatacac attacgaaaa gggacattcc ctgtaaattt
      661 caggcccatc ataatcatcc gggcaaccca aaaaaacagg atatcctggc cggtgactag
      721 ggttgcggtg ggataaaagg ccttgaagtc ttctgttttt tcgggccaac cgagcgtgga
      781 gaaaggccaa agagccgagg aaaaccaggt atccaataca tcaggatctt gaaaaagatc
      841 cgtccctccg caagttggac aggactgtgg tggggttctt gaaacgatag ggttcgcgtc
      901 ggatgggata aagcaactcc ttaaacttgg ctcaggaatg ccgtactctt tagttgcccc
      961 gacgacatgc cctgcttctt ggagattgtc tttgttgcag gttcgacagt accaagcagg
     1021 aatttgatgt ccccaccaaa tctgacggga aatacaccag tctttgattt gttgcatcca
     1081 acccagataa ttatttttcc atccttcggg aataatccga atttgcccgg tttcaaccac
     1141 ctgtatagca ggaattgcca aaggctggat gttgacgaac cattgaggtg aaagatacgg
     1201 ctcaaccacg gttttacatc gataacattt gccaatggct agggcatgat cttcttcccc
     1261 gacgagcaaa tcatgctttt taagggcatc gataaccagg gcgcgggctt ccgggacggg
     1321 tgtaagattg agagcactgc gaagttctgt gtcaacccgg gcttcttgaa ggcccaatgg
     1381 actcattcga gcctggaagt caaatatagc caggcgtgga aggcggtgac gctcacctgc
     1441 ctcaaaatca ttgaaatcgt gcgcaggcgt gattttaact gcaccagttc caaattcccg
     1501 gtcaactaaa atcggatcag ccacgatggg aattgtccgt gtggtgagag gaactcgaac
     1561 ctttttgcca agataatgcc gataccgacg atcctcgggg tggaccgcca cggcagtatc
     1621 tcctaataga gtttctggtc tggtggtggc cacaacgaga gacgccatcg aatcgtcagt
     1681 gagaaagtat cgaatgtggt acagcttccc tttgactgat tcatgctcga cttcaatatc
     1741 agaaagggcg gtaagacagc gggggcacca gttgattaaa cgttcacccc ggtaaataag
     1801 gccttcttca tataggctaa caaacacctc tcgaaccgca agggacagtc cttcgtccat
     1861 cgtaaatcga agacgagtcc aatcgcagga tgcccccaat tgtttcagtt gtccgataat
     1921 gataccgccg gactgggagc gccatttcca gatccgctca atgaatgctt gtcgcccaag
     1981 atgttctctg gattgatttt cctgtgcgag ttgccgttca accacatttt gggtagcaat
     2041 accggcatgg tcagttccag gaatccaaag ggtcttaaat ccctgcatcc gtcgccatcg
     2101 aacaatgata tcctggaggg tcgtattaag ggcgtggcca atatgaaggg atccggtgac
     2161 attaggcgga ggcataacta gacaaaaggt aggggaatta tccataggat caggctgaaa
     2221 aaagtggtgt tcaatccaga attcacccca ccgtttttca atttcagcag cgttgtatgt
     2281 tttatctaat gagggaggtg acatatttaa atattgaatt cctatggaag gaactaaaag
     2341 ttttggatgg tatcacggcc cctacagccc tgcaagaaga cctcttgttc caattatgag
     2401 actatggtag agatatggga ggttatttac tcattttcag gaggatattc aaatgccccg
     2461 aggccg
//
LOCUS       DATIAL010000585        16259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1286254, whole genome shotgun sequence.
ACCESSION   DATIAL010000585 DATIAL010000000
VERSION     DATIAL010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286254"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VLA60_17640"
     CDS             complement(<1..1481)
                     /locus_tag="VLA60_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HSF11238.1"
                     /translation="MFLSRRQFLKVSAGTVAAVALADKALALTALQPVIEVGNPLGEY
                     PDRSWERVYHDQYRYDSSFTWCCSPNDTHACRIRAFVRNGVVMRVEQNYDHQTYEDLY
                     GNRGTFAHNPRMCLKGFTMHRRVYGPYRLKGPLMRRGWKQWMDDGSPEFTPAIQTKYK
                     FNARYLDDMLRVSWDTAFTYLAKAMIVIANRYSGEYGARKLREQGYPPEMIEMMKGSG
                     VRAFKFRAGMPVLGIIGKMGITRMNGGCGALLDSWVRKVGPENAQGGRYWNNYTWHGD
                     QDPSQPWWNGTQNCDVDLSDMRFCKFNTSWGKNFVENKMPEAHWKLESIERGARIAVI
                     TPEYNPTAYRADYWIPIRPESDGSFFLGACKMIVDEGLYDADFVRANTDMPLLVRTDT
                     LQYLDPRDVIKDYQFPDFANTYSGKVQTLKPAEIARLGGIMVWDLNKNQAVPIHRELV
                     GWHFKKSGIDPALTGTHRVRLLTGREVDVMPIFQMYQVHLQDY"
     gene            complement(2748..3692)
                     /locus_tag="VLA60_17645"
                     /pseudo
     CDS             complement(2748..3692)
                     /locus_tag="VLA60_17645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
     gene            complement(3788..4657)
                     /locus_tag="VLA60_17650"
     CDS             complement(3788..4657)
                     /locus_tag="VLA60_17650"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HSF11239.1"
                     /translation="MKSLPRIFQWLVLVGVLLPVGCGVSVKPGERGLRWYPLSEGLMN
                     EPLQNGFYWRAFWNDIYVYSVQGGSFTEEVDALSSDDLQVNVKSAIIIRPIPEEIYFL
                     AQEVGPDWYPKVVRPEFLSAVRSVVSGYPMVSVPEKSTEIAHKVQGVLEEKLKGRHLE
                     IRSIALSDIDWPKIVLEAIERKQAKEQEKEQKEFELIIATRDAEIARRRAKGEGDSLM
                     IREGEAESLRIRAEGQAKAQENILKTLTPEYLQYKLYDSQNSKFVILPTNMQISLLLN
                     TESTQFPQRNTVP"
     gene            complement(4704..4904)
                     /locus_tag="VLA60_17655"
     CDS             complement(4704..4904)
                     /locus_tag="VLA60_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11240.1"
                     /translation="MKNTQQAVNLKAKRHAPFFGPDSEWATSAKLLEKFYEKEAQNRE
                     RLAILHAEAGRLHPHSPGLETR"
     gene            complement(6423..7346)
                     /locus_tag="VLA60_17660"
     CDS             complement(6423..7346)
                     /locus_tag="VLA60_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HSF11241.1"
                     /translation="MKSTVVQMIGVTLGVLGMWAIPVWAYEESQVKQGGTIRGQVTLA
                     GGMPKAMAFNLVTIPDPVFCGRISTGTGWRIVEDFILGTQGSLKDAIVMLKGIEKGKA
                     FELPKVTIEAKDCDFLPFVNVLRDQDELTVINMDPVEHDIQGYETARDRGARVLFNRP
                     LPMNPFHKVLDLMNRHDHLPGKPMIEKIHLQKDRNIFVMQCGFHPYMFSWGVVVDNPY
                     YALTTEDGRFEISDIPPGTYTLSVWHAGMKKYLNREVTIEPNGTISVIFEYQSPVGRR
                     SVHEIQENPHFGLGLLGEEVIIPSLRLQHPS"
     gene            complement(7477..12357)
                     /locus_tag="VLA60_17665"
     CDS             complement(7477..12357)
                     /locus_tag="VLA60_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11242.1"
                     /translation="MLSGKKVYGAVKGVVACMGMVLMMSAMGHAGEEIQEETDSHAGH
                     NVAAASGKTNNWVERLKGQTIVEDSMEGNPDRAANVDRQHERLMSKMGHQMQADLKLI
                     EGSGAYDTATMLHQYGAGPADYLLASDNESEPVSGQPGLCPAGVPEKHYDISAIDVEI
                     TLNRWLDYYVGYMYVLTENLEKVREEEAANAAAREKEGYDPGAVETGLQNNWIQPLVI
                     RGNQGDCVRFTLRNQLITGEGVSLQIGGSSMMVSTTGQPATTTNPDAIAMEGQSVDME
                     WYIPPTMQEGGRQFHSYSNDRELTVMGLMGTFVVEPKGSTYLDPLGAGEPTPMTSGWQ
                     AIIKNGTGPDFREFVLIYHEVGDEAFRPVNKHGDFIPQRDPITDVYRPVARALNYRSE
                     PFGVDNMQTQHEYFGFEDESMAYSAYTFGDPATTVPRSYLGDPAKFRLVHGGSEVFHS
                     HHPHGGSIRWPRSPRAIDEMPLWHTAKNGPVKFPVVRTKTDRVDVEVIGPSEAVDLET
                     ECGSGLCQQLAGDFLFHCHVAHHYIAGMWGYWRVYNTIQQGEVHNDTMPDLRELPDRK
                     GRIKAGVTSDQLIDKKVDWFGKEFKIIEKGTTDWKANPAVVTVKDWVEMQLPPQGKPG
                     HKDDEREQILSYDATVLDWIWNGNRAMTERENTIPNPRYQSPAPGERLPILFEPTTGK
                     IAWPHLRPHFGKRVPFSQNHNPSPWLEMIHLDDDGQMSSYPAKPGENGRWSLCPANAG
                     SKKFNVHFIKTPMMVADKQGDEPAIIDPDGTIYVLHEEEEEVRKNNEKRHPLVVRSNV
                     YDCVDWMLTSEWVDDDNFNMHASKINTHWHFMQFDNQSSDGVITGFSYEQSVRPFTMM
                     EKTTDKGLPLPMNTVFTKPGKKGDVTITVKNAAQYHPKNEILIGADNVKHNEIGRIKA
                     IDGNQIILYRPLEHDHPVDDIVTVEFVRQRFWADADVGTVFWHDHAVGRVTWGHGGFG
                     TMIVEPWGSTYHDPKTGEPIRSGPVADIHTVEPVGYGVNGSFRELMVQLNDSVPHSVN
                     IVTAGNPPGQPIEVALEAGKTISFPMPEHIPMTPMPFLNGGTHTTGGSFNFRAEPVAA
                     RLKANPDFSNIFNSHVHGDPYTPMVRAYLGDTVVFRLLETMTNESMVWTLSGHTFLTE
                     RYAGDANRKNSIHIGIAERYDLVVPQAGGPRLQPGDYIHFNGRSSKFSEGAWGILRVL
                     DKEVPDLQKLPAGYSGRNEIPTPGPVCPAGAPVKSFNVVAMDFPSMNFNTKAPEAVEV
                     DFERNILLQNPEGKIYVLEEDVAKTASGIQPMPLTLRANVGDCLKIHLTNQMKESRAS
                     FSALSLAFDPKDSLGANVGQNPGDQTVAPGESRTYTYYADPFLGEIASLVWDWGNVMH
                     NPRNGLYGAIIIGPKGATYRDPKTGADISTKNSWIADVIVDRTVTGYENRPNYRDAAL
                     FFQDEDNIIGTSFMPYVQNVAGLVGVNYRAEPYLFREEQGCTLDRIFQPCSEEHPEDP
                     VTPIIEAHAGDPVRIHVFGASSEQNGMFSVESHEWPIEPFMRGADQINVVEFSGSEAL
                     DAFIPVAGGPYQRTGDYVWSNQRLPYSQSGQWGYLRVLPGSDQRILSLDGRASGVQEA
                     KLPHEQVVHIKPVELK"
     gene            complement(12936..14129)
                     /locus_tag="VLA60_17670"
     CDS             complement(12936..14129)
                     /locus_tag="VLA60_17670"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HSF11243.1"
                     /translation="MDTPMVKIRPKEIRRRRNRRLALVGVILVAAVLAYGGYWWYYAR
                     FWVWTDNAFIAGNLVTLEAQATGIITQVLVEETQYVEKGDLLIRLDEHQARAKLGRTR
                     GLLGGEVRRIASLFAQRRQIQQKFTSRMARLDLVRHDVARFREALPSGAISEQILENA
                     LDKMRALQAEVREIREEYQSITAEVGGVNIMHHPAVEAAKHEFINAYLEYVRQRIRAP
                     ASGHVAKRKAQVGDQVHPGVPLMTIVPLDHLWVEVNLRETEMEQVRPGQSALINVDVY
                     GKHHTYHGRVEGLVPGTGSVFALLPPENATGNFIHIVERVPVRVAFLKEEILEHPVRP
                     GLSTKTYIDVSEPGRPLGVSLAETSTQEYETNIYADELIDAESLAQEIIRENLVLKRD
                     RLEPA"
     gene            complement(14134..15645)
                     /locus_tag="VLA60_17675"
     CDS             complement(14134..15645)
                     /locus_tag="VLA60_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HSF11244.1"
                     /translation="MRVLSTLRWHGHFAFSLCGLLLCAGCAWIPPGEEPAEFLEPDSM
                     EETLSHATHHEPEDPIPQWPEDRWWQEFGSPELDELIDTGLKNNPGLKVAYARLHEAH
                     ALVRVEGARLLPFLEADAELTAERISEHGVFAALNPEVAGIDIALGLINPLSFRWELD
                     FWGKNRAMMESAMSRELAHHAEWAEVRLRLTTAIARSYFRRIALRQQLEVIEAMVELR
                     RDLLNLAKIRFRSGLDDANSVMRATADLEAANKREAGTREQLDFQQYLLVRLSGHGPD
                     WGEHVFTDRVIMPRRIPLPPELPIGLLAHRPDLAAAKYRAEAAAKLIKVAKTRFLPTI
                     DLNGFVGFRALTLASGAGSLGNLLFSSSSLAYGGGPGLRLPWFEGGRLRGELEAQRRE
                     YDAAVELYNDTLLEAMREVADSLSVWHETRSMLEAHRRLLRSVSDDRHLVEVRFRTGL
                     DDRREVLKQHHAVLDQEFALKALEADQLVSMVGLIEALGGGYPIVQESSQSRK"
     gene            complement(15642..>16259)
                     /locus_tag="VLA60_17680"
     CDS             complement(15642..>16259)
                     /locus_tag="VLA60_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF11245.1"
                     /translation="HELCKRTDVRLLASINFLGFALTLYWIGLYDDPHSFDQIFWPIL
                     LEGLFLGSFFTPMTVLILHRLSGIQVIRAAETANILRIAAGALGITYQEVVHFRRLPF
                     HQLHLANHFGGRQYASFDVLGGFSGKLEGVGLDSSMVNGKLLALVRQYAGILAMSDAF
                     LLASYMFVGLAALVWLAYPTQLPWYQSQDEESLEMEAEALMEEEA"
BASE COUNT         4029 a         4523 c         3722 g         3985 t
ORIGIN      
        1 tcatagtctt gcagatggac ttgatacatt tggaaaatcg gcatgacgtc cacttcccgg
       61 ccggtaagca accgaacccg atgagtcccc gtcaacgccg ggtcaatgcc actcttcttg
      121 aaatgccaac ccaccaattc acgatgaatg gggaccgctt ggtttttatt cagatcccag
      181 accatgattc cacctaacct ggcaatttcc gcaggcttca gggtctgcac tttgcccgaa
      241 taggtattgg caaaatcagg aaactgataa tctttaatca catcacgcgg atctaaatac
      301 tgaagggtat cggtacgaac cagcagtggc atatcagtgt tggcccgcac aaaatcagca
      361 tcatacaagc cctcgtccac gatcattttg cacgccccta aaaagaaaga cccatctgac
      421 tcgggacgaa tgggaatcca gtagtcggct ctatatgccg ttggattgta ttccggggta
      481 ataacagcaa ttcgcgcacc ccgttcaata gactccagtt tccaatgggc ttccggcatc
      541 ttgttttcga cgaagttttt accccaactg gtgttaaatt tacaaaaccg catatcactc
      601 aaatctacat cgcaattttg agttccattc caccagggtt gggaaggatc ctggtcgccg
      661 tgccaggtat agttattcca gtatcgccct ccttgagcat tttcaggccc aacttttctc
      721 acccaactat ccaacaaggc accgcaaccg ccattcatcc gcgtaatgcc cattttccca
      781 atgatcccca aaacaggcat gccggcccgg aatttaaatg cccggactcc ggaacctttc
      841 atcatttcaa tcatctccgg gggatagcct tgctcacgca gtttgcgcgc cccgtattct
      901 ccgctatacc ggttggcgat gacgatcata gccttagcca aataggtaaa cgccgtatcc
      961 caggacaccc gcaacatgtc gtccaaatac cgcgcattaa acttgtattt cgtctgaata
     1021 gcaggagtaa actccgggct cccgtcatcc atccattgtt tccaccctcg acgcatcaag
     1081 ggccccttta aacggtatgg accatacaca cgacgatgca tcgtaaaccc cttcaaacac
     1141 atacgggggt tgtgtgcgaa cgtcccacgg ttgccataga gatcttcata tgtttgatgg
     1201 tcataatttt gctcaacccg catcacgacc ccgttccgca caaacgcccg aatgcgacag
     1261 gcatgggtat cgttggggga acaacaccag gtaaacgaac tgtcatagcg atattgatcg
     1321 tgatagaccc gctcccatga acggtcagga tattcaccaa gagggttacc cacttcaatg
     1381 actggctgca aggccgttaa ggccagagcc ttatcggcaa gtgcgactgc cgccacagtc
     1441 cccgcagaaa ctttcaagaa ttgacgtcta gaaagaaaca ttaaaagacc tccttttttt
     1501 taaaaaactc attacggata gcaataatta ataaagattt ccgtaattgg acttcaaacc
     1561 agacctgcct tgcacctcct ttcttaccat cccgattcaa ccaacaatga acaaacactg
     1621 gtcaccagtg tcaccacatt cctcctctct cttaaatagc aatctttagt ccaaacaatt
     1681 attcccccat tatttttctt aacacattga aaatagaggt taattgaagt taccgcttgt
     1741 tatgggaaaa caatcaggcc gcgaaaatca tcactgaatg ctcgcgaaac ctcgaaattt
     1801 caatggttag gatttttgcg aacgcttttc aggcgttctg tttttctgca aaacatggaa
     1861 agttcatgta tctcaaattg gaattctgac aacccaaccg ttccaagcag agctcatact
     1921 cgggacaaaa tttgacttcg gtttggtgga ttaaaaataa ggtaggacaa aaggaggaaa
     1981 gggggtattt agaatttaat gacagggaca tccttggaaa acgaattgtt ttaaaacaca
     2041 tgacccaaga tacgcacaga tatatagaat acttaagata ccggttgtat ttgtcagtgc
     2101 ctgacagaac atcaggctgg ttgagcggtc aggtccttac tgaatacgtt ctcactccag
     2161 aagctgatga gcatcggccc cactacgccg gagcttggga taatgggcaa catgatggaa
     2221 tttaagactg ccgaccggca ggttttttgg gtttaacagt cacaatccga tcactaccat
     2281 tgagaatgcg ccgatcgttc ctgcaatcaa agtcaaattg ggctaggatg atttttgaag
     2341 acgcactgta ggagcaatct ccaccccctt tacctaaaaa ttctattcca agaggagggg
     2401 agacgtgcat ttcatgaaca caatggcgac ctgcggcgga ttaatgtgca aaattggcag
     2461 gaagagtccc attggattca acgatcactc catggcttca aaacattatt gttccggtct
     2521 gcctagtggc taacgtatag attcctggga ggatagacac ccaatttatg tccagggaaa
     2581 aatacggtct aatcgatact ttcataattg aaaagaacgt gtatctcaca aagaaaatgg
     2641 attgttgggg tccattctct gaatgccatt caaactttga agaattggaa gaagaattca
     2701 gcgcgaataa tttaaaaacc cttgccatag atgatcttat gaaatgccta tgaagccaat
     2761 tgaggtaacg gaaaagccgg tttttttcgt ataaagccta atgtttcgat aatcctctcc
     2821 ggccgacccc aatcactcca aagaacccct tgtagttcga tggtagccag gtgtgagaca
     2881 atcctttcca ggaaattttt tgaaaaattt ttgtggtgca tggcctgata aatcctctct
     2941 aaagtcaagg attcttgagg caatccaatc gctctcctga gttgatgaaa gagttccatc
     3001 atatccggac accagagttc ccccagactc cacagtcggc ttccataggc tgtaacgatc
     3061 atcgtattcc aaattccccc gttccgactt aaagtttgaa tcgttttttg atcgggtttt
     3121 tcaataaatt ctttcaccgt tcggacagga aaccccacat tccctatcaa cgattgaccc
     3181 gggcaaatcc atccatactc tccttctgga gaattgggcg tcgcacccag aaggatgatc
     3241 cgttctttca acctggttac ggctgaaacc gtttcctgta tcattttcgt aaattgttct
     3301 tgaggagcaa taaaatggtc cgagggttaa atggtcacaa tggccttagg gtcccaatga
     3361 ttaatatagg ccaatgccag gaaaacccct gcggccgtgt cgcagtttct gggttgaata
     3421 acgatgcgcc ctttcgtgtg agtctccatc tgccgaaaaa tttcctggtt atgagattta
     3481 tcgataatcg tcaccttatt ttccgaatga ccggcacgat cagctcgatt ccaggtatgt
     3541 tgcaacattg accgctcccc gacaaaggca caatattgct tgggtttggg atatcccaac
     3601 caactttgaa taaatggctt cattcgttgt ccgtctcccc cggccaagat gaccacccac
     3661 tgattccctt cggtgcgttt ttgatcgttc atgatggttc accttttcag taatggccag
     3721 gattaacaat caccaagcaa agcaaaggca cacagacaat acgtttcacc gtcgcatctc
     3781 agtggattca tgggacggta tttctttgag gaaattgcgt tgattcggta ttcagaagga
     3841 gggaaatctg catgttggtt ggaagaataa cgaattttga attttgcgaa tcataaagtt
     3901 tgtattgcaa atattcgggg gtcagcgttt ttaaaatatt ttcctgggcc ttggcttgtc
     3961 cttcggctcg gattctaagg ctttccgctt caccttctcg gatcatcaga gaatctcctt
     4021 cgcctttggc acgtcttctg gcaatttcag cgtctctggt cgcgataatc agttcaaatt
     4081 ccttttgttc tttctcctgt tccttcgcct gttttcgttc aatcgcctcc agaacaattt
     4141 tgggccaatc gatatcagac agggcgatac tccgaatctc aaggtgacgc ccctttaatt
     4201 tttcctccag gactccctga accttatggg caatttccgt gctcttttcg ggtacactca
     4261 ccatgggata tcctgaaacc acactccgaa cagcagaaag gaactcaggc cgtacaacct
     4321 tgggatacca atcaggcccg acttcctggg ccaaaaaata aatttcttcc ggtatgggac
     4381 ggatgataat ggcgcttttc acattcacct gcaaatcatc cgaactcagt gcatccactt
     4441 cctccgtgaa gcttcccccc tgaaccgaat agacataaat atcattccag aacgctcgcc
     4501 agtaaaatcc attttgaagc ggttcgttca tgagaccttc agataagggg taccatctca
     4561 gaccccgttc ccccggcttc acggaaaccc cacaacccac gggtagcaga acgccgacaa
     4621 ggactaacca ttgaaaaatc cgaggaagag atttcatgtt ggcctcctta agcaagagac
     4681 tcaaacgtct ctctcacaat tctctaacgc gtttcaagac ccggagagtg agggtgaagc
     4741 ctgcctgcct cggcatgcag gatggctaaa cgttcccgat tctgcgcttc tttctcatag
     4801 aatttttcca acaatttggc gcttgtggcc cattccgaat cagggccaaa gaatggggca
     4861 tgtcgtttgg ccttaagatt cacagcctgt tgtgtatttt tcacctagaa gaccctttgc
     4921 cgatcagact gggcgcccat agacggtgga tctgggaaag gggcggaatc atgaagattg
     4981 atgagaggac tggtcccatg acatcccact ttgaccaata ttcctattag caggaccgct
     5041 tgaaggctgg ctagtaataa tcgagtcaaa gagtagccct cccttgccaa gttcctgaag
     5101 attcttttct tcttagcctc tacccgtcat gtttcctaga cgactcgtaa gttcaattct
     5161 taaaacaccc tggacaggaa aactgaattt tcactccgtt ttatgatact ccagcattct
     5221 ttaataactc cgaccattat gtaggtaact cgctaatgat caaaattcat accagatgat
     5281 taacaggttg agcagccaca ataagagttg gacaacaagg acttatcgaa atgaggcgga
     5341 tctcagccaa tgtcatttgt gcgataccac acaatccggc tcgttcatat tcgctccaca
     5401 cgcaggcaag tcgggatgaa ccgacaaatc agcaatgtgt ggtttcgtgg agcccttgtg
     5461 ggcaagaggt atctgtattg ccggaaaggg gcactcgaag agaaaatgag gaaaatttga
     5521 ggagccggca gctataatct ccgccaaaag ggcgggcctt ccacgtttgt gtgaggggta
     5581 agaaaatata cattcagcca attccgtaac gactcttgcc aattttatcc ctcatcctta
     5641 cttttcccct ctcacgtttt atccctcacc ttgcacgtat tatggaaccg gggaccccgt
     5701 tgcgacctct ctgctgaagc ctaggcatgg agacgtcggc tccttcgcct agactcaggg
     5761 ccatccaggt agcatcccta tagcgatgag gatattgcgg gccatgatca ttcgcgtttc
     5821 attatgcttt gtatcgtaac tcgaacggtg ggggtgaatt gagaccaaac gatagggaag
     5881 cgttactgcg gctattgaat atttcaaata ttcggccatt ttcatgggaa tatgtgtgcc
     5941 gagacccagg aaggggaaat gttaggagta ttcaaaaagg ctcgtccagt gctggactga
     6001 agcttgacga gagaaaggct gcagccattt tttaggggag cggaacgcac ggaagggtac
     6061 gtgagcacgc ccaagcggcg acctgcatac gcgtttgcgg gtcaatacgc ttcagcgggt
     6121 aggtggagcc tctccggcaa agcaggaaac accgctggcg gagtttttca actctccctc
     6181 accgggagag ttggcattcc agcatgcttg cccttctcca ggatagtaac cgggaagacg
     6241 ggtcaccata aggaatttgc atactggcaa aaaataaaag atccccgcag caagccacgg
     6301 ggaatagagt tgattcaatt gtctagaaat agatggaaag aaacaacgcg cctcaaaatt
     6361 tgcccgttct taaaaacaga aacaccctgc taccccatgc gataacctgt tcctgatgtt
     6421 tgtcatgagg gatgttggag acgaagtgaa gggatgatta cttcttcccc caataggccc
     6481 aatccaaaat gggggttttc ttgaatttca tgaacactgc ggcgacccac gggtgattga
     6541 tattcgaaaa tcacagaaat tgtcccattc ggttcaatag tcacctcacg gttgaggtat
     6601 ttcttcattc cggcatgcca gaccgataac gtataggttc ccggtggaat gtcggatatc
     6661 tcaaaccggc catcctcggt tgtaagcgca taatagggat tgtccaccac caccccccaa
     6721 gaaaacatat agggatgaaa cccgcattgc atcacaaaga tattccgatc cttttgcaaa
     6781 tgaatttttt cgatcattgg cttgccgggt aaatgatcat gtctgttcat caaatccaat
     6841 actttatgaa atggattcat tggaagaggg cgattaaaca agaccctcgc cccacgatca
     6901 cgagccgttt cgtatccctg gatatcatgt tcaactggat ccatattaat gaccgtcagc
     6961 tcatcctgat ccctcaagac attgacaaat gggagaaagt cacaatcctt cgcttcgatt
     7021 gtcacttttg gcaactcaaa cgctttgcct ttttcaatac ccttgagcat cacaatcgcg
     7081 tccttcagcg agccctgtgt tccaagtatg aaatcttcaa caatacgcca tccagttccg
     7141 gttgaaattc tcccacaaaa aaccggatca ggaatcgtga ccaaattaaa ggccatggct
     7201 ttaggcatcc ctccagctaa agtcacttgc ccacgaatcg ttcctccttg tttgacctga
     7261 ctttcttcat aggcccaaac cgggatggcc cacattccca ggacgcccaa cgtcacccca
     7321 atcatctgta ccaccgtgct cttcatttat tcctccgcca gcataaaatt ccaaatgttt
     7381 ttcaccatct tgtcttatgg ggttgtgtga ccagcacggg gaagccgtgt atcggcttcc
     7441 ccgttcccaa acccgtcaac taaacataat gccggctcat ttcagttcga cgggtttaat
     7501 atggaccact tgctcgtgtg gcaacttggc ttcctgaacg ccagaagccc ttccatccaa
     7561 tgaaaggatt cgttgatcac tccctggtag gacgcgcaaa tatccccatt gccctgattg
     7621 ggaatagggc aaccgctggt tgctccagac gtaatccccg gtgcgttgat atggcccacc
     7681 tgcgaccgga atgaaggcat ccaacgcttc tgaccccgaa aactccacga cgttgatttg
     7741 atcggctcct cgcatgaatg gttcgatcgg ccattcatga ctctccacac taaacatccc
     7801 gttctgctca ctgctggcac cgaaaacatg gatgcgaacg gggtcaccgg catgggcctc
     7861 gatgatcggc gtgacggggt cttccgggtg ttcctctgag cagggctgga atatcctgtc
     7921 caacgtacaa ccctgctctt cccggaataa atagggttcc gctcgataat tcaccccgac
     7981 caaaccggcc acgttctgca cgtaaggcat aaagctcgta ccgataatgt tgtcctcgtc
     8041 ctggaagaac aaggccgcgt cccgataatt aggtcggttt tcatatccag tcaccgttct
     8101 gtccacgatc acgtcggcaa tccaactatt tttagtggat atatccgcac ctgttttcgg
     8161 atcccgatac gtcgcgcctt tcggcccaat gatgatggcg ccgtagagcc cattccgtgg
     8221 attgtgcatg acattgcccc aatcccaaac caacgaggca atctccccta aaaacggatc
     8281 cgcgtaatag gtgtacgtcc ggctctcgcc aggtgcaacc gtctgatcgc cggggttctg
     8341 tccgacgttg gctcccaacg aatccttagg atcgaaagcg agggaaagcg cggagaacga
     8401 ggcgcggctt tccttcattt gattcgtcag gtgaatcttg agacagtcgc ccacattggc
     8461 tctcagagtc agcggcatcg gttgaatgcc actggccgtc tttgcgacat cttcttcgag
     8521 cacatatatt tttccctccg gattctggag cagaatgttc cgttcgaaat cgacctctac
     8581 ggcttcgggt gccttcgtgt tgaaattcat ggaaggaaaa tccatcgcca caacattaaa
     8641 tgatttcacc ggagccccgg cgggacagac cggccccggc gtcggaattt cattacggcc
     8701 ggaatagcct gccggcagct tttgaagatc aggcacctct ttgtccaata cccgcagaat
     8761 tccccaggct ccttccgaga actttgaaga acgtccgtta aaatggatgt agtcaccagg
     8821 ctggagccga ggcccccctg cctgcggaac cacgagatcg tatcgctctg cgataccaat
     8881 gtggatggaa tttttccggt tggcatcacc ggcataccgc tcggtcagaa aggtatgccc
     8941 tgacaaggtc cacaccattg attcgtttgt catggtctcc agcaaccgga acaccaccgt
     9001 atcgccgaga tacgccctga ccatcggcgt atagggatcc ccatgaacat ggctattaaa
     9061 gatgttcgag aaatccgggt ttgccttgag tctagccgcg accggttcgg cccggaagtt
     9121 aaaactgcca ccggtggtgt gggttccccc gttgaggaac ggcatcggtg tcatgggaat
     9181 atgttcaggc atgggaaatg agatcgtctt tcccgcttcc agcgcgacct caatcggctg
     9241 ccccggagga ttgcctgcag tgacgatgtt gacggaatgc ggcacactgt cgttcaactg
     9301 caccatcagt tcacggaagc tcccgttcac tccataccct accggttcaa ccgtatggat
     9361 atccgcaacc ggtccacttc ggatcggttc gcccgttttg ggatcgtgat aggttgagcc
     9421 ccagggttca acaatcatcg tcccgaaccc tccgtgtccc caagttacac ggccgaccgc
     9481 atggtcatgc cagaacacag tacccacatc cgcatccgcc cagaatcgtt gtcgtacgaa
     9541 ttccacggtg acaatgtcat ccaccggatg atcatgctct aacggccgat acagaataat
     9601 ttgattccca tctatggctt taatgcgccc gatctcgttg tgcttcacgt tatcggcacc
     9661 gatcagaatc tcgttcttcg gatgatactg tgcggcattt ttcaccgtga tagttacgtc
     9721 acctttcttg cccggcttgg tgaataccgt attcatcggc agtggcaggc ccttgtcagt
     9781 cgtcttctcc atcatcgtaa agggtcgcac cgattgttca tacgaaaagc cggtaatcac
     9841 accgtccgac gattgattgt caaactgcat gaaatgccaa tgggtgttga tcttcgacgc
     9901 atgcatgttg aaattgtcgt catcaaccca ttcgcttgtc agcatccaat cgacacagtc
     9961 gtacacgtta gatctcacga ccagcggatg tctcttttca ttattctttc gaacctcttc
    10021 ctcttcttca tgcagaacat aaatcgtccc atccggatcg atgattgccg gttcatcccc
    10081 ttgtttatct gccaccatca tgggggtttt aatgaaatgc acgttaaact ttttggagcc
    10141 ggcattggct ggacacaggc tccaccgtcc gttttctcca ggctttgccg gataagaact
    10201 catctgacca tcatcatcta aatggatcat ttcgagccaa ggagaaggat tatgattttg
    10261 agaaaatggc acccgttttc cgaaatgcgg cctcaagtgt ggccatgcga tcttgccggt
    10321 ggtgggctcg aacagaatcg gcaagcgttc gccgggagcc ggagattggt atcgtggatt
    10381 ggggatagtg ttttcccgtt cggtcatcgc ccggtttccg ttccaaatcc agtccaacac
    10441 cgtcgcatca taggagagga tctgctctct ctcatcatct ttgtgaccgg gcttgccttg
    10501 aggcggcagc tgcatttcca cccagtcctt caccgtgacg acagccggat tggctttcca
    10561 atccgttgtc cccttctcga taattttaaa ttctttgccg aaccaatcga ctttcttgtc
    10621 gataagctgg tccgaagtga caccggcctt gatccgtccc ttgcggtccg gcaattcgcg
    10681 aaggtccggc atcgtatcgt tgtgaacttc tccctgctgg atggtattgt aaacccgcca
    10741 atagccccac atcccggcga tatagtgatg cgccacgtga cagtgaaaca gaaaatcccc
    10801 ggccaattgc tggcagagtc ccgatccaca ttccgtttcc aggtcaacgg cctcggacgg
    10861 cccgatcacc tccacgtcga cacggtccgt ctttgttcgg acaaccggga acttcaccgg
    10921 tccgtttttt gcggtgtgcc acaggggcat ttcgtcgatc gcacggggac tcctgggcca
    10981 gcgaattgac ccgccatggg gatgatggga atggaaaacc tccgatcccc catgcaccag
    11041 gcggaacttc gccggatcgc ccaaataact gcgcggcacg gtggtggccg gatcaccgaa
    11101 ggtataggcg ctgtaggcca ttgactcgtc ctcaaatccg aaatactcat gctgcgtttg
    11161 catgttgtcc acaccaaacg gttcgcttcg gtagttcaac gcccgcgcga cgggccggta
    11221 cacatcggtg attggatccc gctgcggaat aaagtcacca tgcttattga ccggacgaaa
    11281 ggcttcatcg cccacttcat gataaatgag tacgaactcg cgaaaatccg gaccggtgcc
    11341 gtttttgata atcgcctgcc aaccactggt catcggtgtc ggttcccctg cgccaagcgg
    11401 atccagatat gtcgaaccct ttggttccac cacaaaagtc cccatcaggc ccatcaccgt
    11461 caactcacga tcattgctgt aactatgaaa ctgccgtccg ccttcctgca tcgtcggtgg
    11521 aatgtaccac tccatgtcga cgctttggcc ttccatcgcg atcgcatcgg ggttcgtggt
    11581 tgtcgccggt tggccggttg tgctcaccat catacttgat cccccgatct gcaaactcac
    11641 cccttctccg gttatcaact gattgcggag agtgaacctc acgcaatcac cctgattgcc
    11701 ccggattacc aatggctgaa tccagttatt ctgcaaaccg gtctcaaccg cgccggggtc
    11761 atatccctcc ttctcacggg cggcggcatt agccgcttct tcctcccgca ccttctcgag
    11821 attttcggtg aggacataca tgtagccgac atagtagtcg agccatctat tcaacgtgat
    11881 ttccacgtcg atggccgaaa tatcgtagtg cttttcgggc acaccagccg ggcacagtcc
    11941 aggctgaccc gagacgggtt cactttcatt gtccgaagcc agaagatagt ctgccgggcc
    12001 ggctccgtat tgatggagca tcgtggcggt gtcataggcc ccactaccct caatcaactt
    12061 caaatcggcc tgcatttgat gtcccatttt cgacattaac cgttcatgct gtcgatccac
    12121 gttcgccgca cgatcaggat tcccctccat cgagtcctct acgatcgtct ggcctttcaa
    12181 cctctctacc caattattcg tctttcctga cgctgccgcc acgttatgac cagcatggga
    12241 atccgtttcc tcctgaatct cctctcccgc atgtcccatg gcactcatca tgagcaccat
    12301 ccccatacac gcgacaacgc ctttcactgc gccatacacc ttcttccctg acaacataaa
    12361 acatcccctc ctcataaaaa atcgatcatg gccctttaag ttattaacgt ctccgggaac
    12421 atgaacccct tctatagcga taacattctc ttctccaatg catttcctct ttgattcatg
    12481 accatacttt ttcgttactt tccgataagc aaatagcgag ccgcccaacg cttcatccac
    12541 tatctacaaa ttggccttat tttcgtaacc tcttttggca cataaaaatc aatccgctgg
    12601 aatgccacga aagatcgctg ccgcccacca cgattccacc accacttttc gtgggaaaca
    12661 tttttactgc aatgctcaag gattaacgag caggcatgat tgaagattct ttaagaatga
    12721 agaaaggaat ttcagaatga ggaccaggct catccggttg gacggaaaga acagaacaaa
    12781 ggccttcaat aggcaagcag tggaatgggg gtacttaaac cttcatcaac aaattacaga
    12841 aaagattgtg gaccgaaggg agaatacttt ttccttccac cgtcaagttg actacctccc
    12901 gatacatagg ttttaaaccg acacgactcc accccctatg ccggttcaag ccgatcgcgt
    12961 ttcaaaacaa ggttttccct gatgatttct tgggccaagg actctgcatc gataagttca
    13021 tcggcataga tgttcgtctc atattcctga gtggacgtct ctgccagcga aactcccaga
    13081 gggcgtcccg gctcgctcac atcgatatag gttttggtcg acaatcccgg acgaacgggg
    13141 tgctccagga tctcttcttt caaaaaagca acccgcacag gcacgcgttc cacgatatga
    13201 atgaaattgc cggttgcatt ttcaggaggc aacaaggcga agacgctgcc ggtgccgggc
    13261 accaaccctt cgaccctccc atggtatgtg tggtgcttcc cgtatacatc cacatttatc
    13321 agtgccgact gaccgggacg gacctgctcc atttcggttt cccgtaaatt tacttccacc
    13381 cacagatgat ccagaggcac gatcgtcatc aatggaacac caggatgaac ctggtctccg
    13441 acctgggcct ttcgcttggc cacatggccc gaagccggcg cccgaatgcg ctgccggacg
    13501 tattcaagat aggcgttgat gaattcatgc ttggcggctt ctacagccgg atgatgcatg
    13561 atattcacac ccccgacctc cgcggtgatg gattggtatt cctcgcggat ttccctgacc
    13621 tcggcttgaa gggctcgcat cttatcaagg gcattctcaa ggatttgctc agatatggcc
    13681 ccacttggaa gcgcctcacg gaagcgggcc acatcatggc gtactaagtc aagtcgagcc
    13741 attcttgaag taaatttttg ctggatctgc cgcctctggg caaacaatga cgcgattctg
    13801 cgcacctccc cccccagtaa gccccgcgtc cgacccaatt tggcccgggc ctggtgttca
    13861 tcaagacgaa tgagaagatc tcctttttcg acatactggg tttcctccac gaggacctga
    13921 gtgataatgc ccgttgcctg ggcctccaac gtgacgaggt tgccggcaat aaaggcatta
    13981 tcagtccaga cccagaaacg ggcgtagtac caccagtacc cgccgtaagc caataccgcc
    14041 gcaacgagaa tcacgccaac caacgccagc cgacggttac gtcggcgacg aatctcctta
    14101 gggcgaattt tcaccatagg ggtatccatg atttcatttt cttgattgag aggattcttg
    14161 aacgatgggg tatcccccac ccagggcttc gatcaaaccc accatcgaca caagttgatc
    14221 ggcttccagg gccttcaagg cgaactcctg gtccaaaacc gcatgatgct gttttaacac
    14281 ctcacggcga tcgtctagac cggtgcgaaa gcggacttca acaaggtgcc ggtcgtcact
    14341 caccgacctc agcagacgac ggtgtgcctc cagcatcgag cgagtctcat gccagacact
    14401 cagactgtcg gcaacttccc gcatggcctc cagcaaggtg tcgttataga gttccactgc
    14461 cgcgtcgtat tctctccgtt gcgcctccag ttccccgcga agccgacccc cttcgaacca
    14521 gggaagccgc agaccaggcc cacccccata ggcgagactg gatgatgaaa acagaagatt
    14581 acccaacgaa ccggccccac tcgccagagt caaggccctg aagcccacga acccattcaa
    14641 gtcgatagta ggaagaaatc gagttttcgc caccttgatc agcttggctg cagcttcggc
    14701 tcgatattta gcggcggcga gatccgggcg atgcgccaac agcccgatcg gcaactccgg
    14761 cggcaaggga attcgccggg gcataatcac cctatcggtg aacacatgct ctccccaatc
    14821 agggccatga ccgctcagcc tgaccagcaa atattgttga aagtccaact gctcccgagt
    14881 accggcttca cgcttgttcg ccgcctctaa gtccgccgtg gcccgcatga ccgaattggc
    14941 atcatccagg cccgaccgaa accggatctt ggccagattc aggaggtcgc gacgaagctc
    15001 caccatggcc tcgatcactt caagttgttg gcgcaacgcg attctgcgga agtacgaccg
    15061 cgcgatcgca gtggtcaacc gcaaccgcac ctctgcccat tccgcatgat gggccaattc
    15121 cctgctcatc gcggattcca tcatggcgcg attcttcccc cagaaatcca actcccatcg
    15181 aaaactcaat ggattgatca ggcctaacgc aatatcaatc ccggccactt caggattgag
    15241 ggccgcaaac acgccatgct cggaaatccg ctctgcggtc agttcagcat cggcctccag
    15301 aaacggcagc aacctcgcgc cttcgacgcg gacaagggca tgcgcctcgt ggagacgggc
    15361 ataggcaacc ttgagacccg ggttattctt gagacctgtg tcgatcaact cgtccagttc
    15421 aggactcccg aactcttgcc accaacgatc ttccggccat tgcgggattg gatcctccgg
    15481 ttcgtgatgg gtcgcatggg aaagtgtctc ctccatggaa tccggttcta agaattcggc
    15541 cggttcctcg ccgggaggaa tccaggcgca accggcgcag aggagcaagc cgcacagact
    15601 gaacgcgaaa tgcccatgcc acctcaaagt ggacagaact ctcatgcctc ctcctccata
    15661 agtgcttcag cctccatctc gagcgactct tcatcctggc tttgatacca aggcaattgg
    15721 gtaggatagg ccaaccagac gagggcggcc aatcccacaa acatgtagct ggccagcaga
    15781 aacgcatcgc tcatggccag aattcccgca tattgcctga caagtgccag gagtttgcca
    15841 ttcaccatgg aagaatcgag cccgacgccc tctagcttgc cagagaatcc tcccaacacg
    15901 tcgaaagagg catactgcct tcccccaaaa tggttcgcca aatgaagctg atggaaggga
    15961 agacgtcgga aatgcacaac ctcctggtag gtaatgccta acgccccggc ggcgatgcgc
    16021 aagatattgg ccgtttccgc tgcacgtatc acctggatgc cggataatcg atgcaggatg
    16081 agcacagtca tcggggtgaa aaacgatcca aggaaaagac cttcgagcag aatgggccag
    16141 aaaatctgat caaatgagtg aggatcgtca tacagcccta tccagtacag agtcaaagca
    16201 aacccaagaa aattgatgct cgcgagcagg cgaacgtcgg tccgtttgca gagctcatg
//
LOCUS       DATIAL010000586         5119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1070526, whole genome shotgun sequence.
ACCESSION   DATIAL010000586 DATIAL010000000
VERSION     DATIAL010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070526"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /gene="egtD"
                     /locus_tag="VLA60_17685"
     CDS             <1..932
                     /gene="egtD"
                     /locus_tag="VLA60_17685"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HSF11246.1"
                     /translation="DPDLVKTQITRGLQASPKTLPSKLFYDEQGSTLFEQICELPEYY
                     QTRTEHQLLTNCADEIVALSGAEELVELGSGAATKTRVLLDAMANVGQLRYFVPFDVD
                     ETIVRRVSEELVREYPGLHIHGVVGDFLFHLEHIPEGGKRLVVILGGTIGNLPALAAQ
                     EFLSSVNSEMASGDYFLLGVQLITERSRLEAAYNDRQGITAKFNKNIIRVLGHHFGSQ
                     ADPDSFDHIARYNNDEHRIEMWLRSREDQILDIQDLGLQVHLKQGEEIRTEISTKYDR
                     PLAETLLAASGFDLVKWYSDPDSLIGLALAKKP"
     gene            1025..2077
                     /locus_tag="VLA60_17690"
     CDS             1025..2077
                     /locus_tag="VLA60_17690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HSF11247.1"
                     /translation="MIYLNPAGLAPFHPDVQREISRTLDTFSHLLYAEAGIQYYRDTL
                     QECRRTIADWLRLKDEQRLAFMPNTTTACSLVLSRINWKPGNVLLTTTHENSTILNEI
                     DKLSDRGVRVITLDPDVPSGLLPALERILNKQPIQAIVISHVSHFDGRIFPITAIQDL
                     AQSHSTLLIVDGAQAIGHIPVSFQEISPYAYFFPGHKWCTGPMGTGALIVGKDRHQET
                     QPYWAEYELGTQNIGLIAGFAKACHLKAQQDPHNHILEQVRKEWKVCLGHYPGLRIIE
                     WDVAHAPGILSFACLDEHTEQAMHTLSSTHSIAWKTLTHPSHPSNLSIRISWSTDTSS
                     VDIRSTLALFSSLSNE"
     gene            complement(2162..4426)
                     /locus_tag="VLA60_17695"
     CDS             complement(2162..4426)
                     /locus_tag="VLA60_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HSF11248.1"
                     /translation="MKRCPFSPTLLLLLIVVLFVGHTLIGTMPAAFGAGAPSVKRALL
                     IGIGKYQVLPRLPGSKNDIDLVHQVLLSRYGFAEQDIHIIRDEAATREGVLAGLHRIV
                     TDAGPNDVVYIHYSGHGSQVEDLNGDEPDDQLDETIVPADGRTEGVPDITDDELDEIL
                     SRLKTSKAVVVLDSCHSGTATRGLEVRVRSVPADRRVDLYKKGGVATRAIVPVNLHSY
                     VLFSGAASHEEALDGPVDGRYHGFFTHSLFKSLQSAPLDASTRKIFSGAKQELKRIQN
                     QFGRTSMPEPQLEASKARMELPFFSALDEPPGLVKDQTGSARRSWVAVQPQTGDHVQL
                     VNALSLGADPGSVWGIYPEGEVAFDPAKAQAFAVVQAADGQNAVAVISPKQAEISSKS
                     RAVLVAPAPKSKAIPVALRNVPPVQAKQLKELLGKHVGDVKFVGDQEFARFIVESRGD
                     GLHVLSADGTMELMSLPAKPGTQAIAALSQVLVQSRNASQLLNLENPTSQMSVSVRVV
                     QVGERGIAVVSDKMEAPVYHIRKANEPRSLSNSLQLEVTSDTDAYITIVDVDAEGNVN
                     VLFPNSYQNPRYYPDGFIKAGRTVLLPDSLQSGNQAGFHFDYANPPGVDTIRVFASTT
                     SELAQRIRQAVAGGNSHGLGSPQSTNSLAHLASLRQELVGSVTRGLITVPDDSVPPVS
                     QPAMSTVEQTPLAMVDQLPPNNSMPESFGYIQDRPAESFPPDPSNHLDAQAMPVGLPI
                     SDWTAVSVTVLVQP"
     gene            4799..>5119
                     /locus_tag="VLA60_17700"
     CDS             4799..>5119
                     /locus_tag="VLA60_17700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HSF11249.1"
                     /translation="MSSTNWQDLTDSDLLQSCIPNNQEAFAILYQRYEKRVFHYLMTI
                     LNEATLAEETLVEVMLAIWKGLQTFQGQSKVSTWIFGIAHHKAVDALRKVTSQQRGGI
                     PLEDI"
BASE COUNT         1289 a         1485 c         1178 g         1167 t
ORIGIN      
        1 aagatccgga cttagtcaaa acccaaatca cccgcggtct gcaagcaagc cccaaaaccc
       61 tcccttcgaa actcttttat gatgaacagg gatccaccct gtttgaacaa atatgcgaat
      121 tgccggaata ttatcaaaca cggaccgagc atcagttgct caccaattgc gccgacgaaa
      181 ttgtggctct cagcggagct gaagaacttg tggaattagg atctggcgca gccacaaaaa
      241 cacgggtgct cctcgatgcc atggccaatg ttggccaact gcgctatttc gtcccgtttg
      301 atgtcgatga aaccattgtc cgacgggtct ccgaagaact ggtccgcgag taccccggtc
      361 tccacattca tggcgtggta ggagattttc ttttccattt ggagcatatt ccggaaggcg
      421 gcaaacgcct ggtggtcatc cttggaggaa ccattggcaa ccttcctgct cttgccgctc
      481 aagagtttct ctcctccgtg aactcagaaa tggcatccgg ggattatttc ttattgggag
      541 tccagctcat cacggaacga agtcgcctgg aagccgcata taatgacaga caaggcatta
      601 cagcaaaatt taacaaaaat attattcgtg ttcttggtca tcatttcgga tctcaggctg
      661 atccggattc gttcgaccat atcgccagat acaacaatga tgaacatcgc attgaaatgt
      721 ggctccggtc acgagaagat caaattctgg acattcagga cttaggatta caagtccatc
      781 tcaagcaagg ggaggaaatc cgcaccgaaa tcagcacgaa atatgaccgc cccttggccg
      841 aaacacttct cgcggcttcg ggttttgact tggtcaaatg gtactcggac cctgacagcc
      901 tgattggcct cgcactcgca aaaaaaccct aagccggatg attcgagaga ggcacgccct
      961 tttctatcag gattcagacc cgacctaacc tcacaaccct agggtactcc gccaacctac
     1021 tcccatgatt tatctcaacc cggctggact cgctccattc catcctgacg tgcagcggga
     1081 aatttcccgg acactcgaca cgttcagtca cttgttatat gcggaagccg gcattcagta
     1141 ttaccgggac acgcttcagg aatgccgacg gaccattgcg gattggttga ggttgaagga
     1201 tgaacaacgg ctcgccttca tgcccaatac cacaactgca tgcagcctgg tcctttctcg
     1261 gatcaattgg aaacctggaa atgtccttct gaccaccacg catgaaaatt ccacgattct
     1321 caatgaaatc gacaaactca gtgaccgtgg agtccgcgtt attacactcg acccggatgt
     1381 cccctccggc ctccttcccg cgcttgaacg cattctaaat aaacaaccca ttcaggccat
     1441 cgtcatcagt cacgtctctc attttgatgg gcgtatcttt cccatcaccg caatccagga
     1501 cctggcgcag tcacattcca cgctgctcat cgtagacggg gcacaagcca ttggacatat
     1561 tccggtttct tttcaggaga tttctcccta tgcgtatttt ttccctggcc ataaatggtg
     1621 cacaggccca atgggaaccg gtgcgctgat tgtcgggaaa gaccgtcacc aggaaacgca
     1681 accctattgg gccgaatacg aattgggcac ccagaatatt ggcttgatcg ccggatttgc
     1741 caaagcgtgc catctcaagg ctcaacaaga tccacacaac cacatccttg aacaggtgcg
     1801 aaaagaatgg aaagtctgcc tcggacacta tcctggcctt cggattatcg aatgggacgt
     1861 agcgcatgct cccggcatct tatcgtttgc ctgccttgac gaacacacgg aacaggctat
     1921 gcacaccctt tcctccactc attccattgc ctggaaaacc ctcacacatc cctcccatcc
     1981 ctcaaacctt tcgatccgta tctcatggtc taccgacaca tcaagtgtcg acatccgttc
     2041 gacccttgca ttgttttcct cactctcaaa cgagtgaggc cccctacaaa tcacataggg
     2101 ggcctcatgg tgtcatcacc aagaaacaga ggaacctttc cttggctcat ctcattgtgc
     2161 tctatggttg aaccagaact gtaaccgaaa ccgcggtcca atcagagatc ggcaacccaa
     2221 ccggcatggc ctgggcgtcc aaatgattag aaggatcagg tggaaaagat tccgcagggc
     2281 ggtcttgaat atatccgaaa ctttcgggca tggaattgtt gggcggcagt tgatcgacca
     2341 tggccaatgg cgtttgttcc acagtgctca tagcgggttg ggaaaccgga ggcacggaat
     2401 catcaggaac ggtaatcaac cctcgagtca cacttccgac taactcctgc ctcaaactgg
     2461 ccaagtgggc taaggagttg gtgctttgag gactcccaag gccatgggaa ttgccacccg
     2521 ccaccgcctg gcgaatccgt tgggccaatt cagaagtcgt actcgcaaaa accctgatcg
     2581 tatccacgcc cggaggattc gcataatcaa aatggaatcc cgcttgattt ccggattgca
     2641 atgaatcggg caacaagacc gtacggcccg ccttgatgaa gccatcagga taataccgtg
     2701 gattttgata agaattgggg aacaacacat tcacgttccc ttccgcgtcc acatccacga
     2761 tcgtaatgta cgcatccgta tcggaagtga cttccaattg cagactgtta gacaacgacc
     2821 ggggttcatt cgccttccgg atgtgataga ccggggcctc cattttgtcg ctcaccacgg
     2881 cgatcccccg ctctccgacc tgaaccaccc gcacactcac gctcatctga ctcgtagggt
     2941 tttccaggtt caacagttgc gacgcattcc gtgactgcac caacacttga gacaaggcag
     3001 caatcgcttg agtcccgggc tttgcaggca atgacatcaa ctccatggtg ccatccgcgc
     3061 tcaagacatg cagcccgtcc ccccggcttt ctacaatgaa gcgggcgaat tcctgatctc
     3121 ccacaaattt cacatcaccc acgtgcttcc ccagcaactc tttcaattgc ttggcttgca
     3181 cgggtggaac attccgaaga gcaaccggaa tggccttgga cttcggagcc ggagcgacga
     3241 gaaccgcacg gcttttcgaa gatatttcgg cttgtttcgg cgaaatcacc gccacggcat
     3301 tctgaccgtc cgccgcttgc accaccgcaa aggcctgggc tttggccgga tcaaatgcca
     3361 cttctccttc cggatagatt ccccataccg aaccgggatc ggctccgagc gacagggcat
     3421 tcactaactg cacatgatcc cctgtttgcg gttgcaccgc cacccaggaa cgacgggcgg
     3481 acccggtctg atccttcacg aggccaggcg gttcgtcaag agccgaaaag aatggcaact
     3541 ccattctcgc cttagaagct tcaagctgag gttcgggcat actggtccgc ccgaattgat
     3601 tttgaattcg cttgagttcc tgtttggctc cggaaaaaat cttgcgggtc gacgcatcca
     3661 ggggagcgga ttgtaaactc ttgaagaggg agtgggtaaa aaatccatgg tatcggccgt
     3721 ccaccggccc atccaaggcc tcttcatggg aagccgctcc actgaatagc acatacgaat
     3781 gaagattcac cggcacaatg gcccgtgtcg ccactccacc ttttttatac agatccaccc
     3841 gacgatccgc cggaacggat cgcacccgca cttccaaccc ccttgtggca gtcccggaat
     3901 gacaggaatc gagaacaacg accgccttgg acgttttcaa tcgtgacagg atctcgtcca
     3961 actcatcatc ggtaatatcg ggaacgcctt ccgtccggcc atcagccgga acaatcgtct
     4021 catccaattg atcatccggt tcatctccat tcagatcctc aacctgagat ccatgtcctg
     4081 aataatgaat gtagaccaca tcattgggac cggcgtctgt cacaatccga tgtaaaccgg
     4141 ctaggacccc ctcccgggtt gctgcttcat cccggataat gtggatatcc tgctcggcaa
     4201 acccataccg ggacagcagg acctgatgca ccaaatcaat atcatttttt gatcctggga
     4261 gccgtggcaa aacctgatat tttccaatgc cgatcaagag agctcttttc accgaaggcg
     4321 caccagcccc gaatgccgca ggcatggtcc cgattaacgt atgcccgaca aacagaacga
     4381 caattaaaag aaggagcagt gtgggggaaa atgggcacct tttcatgata aacctcttat
     4441 caagcctgcc gtgaaatgga tccatagtct atcggtcttt gtgtcgtctc tcaacctttt
     4501 gttatgtggt ttgtctcccc atttccccaa aaggttcaag ggcaaccata agagaaaacc
     4561 aatcaaccct tcatcattgt cgaattcata catgacacag aggggagatg gggtattgtg
     4621 actgattcaa aatcaatgct aaacagccgc tgtcccttcc aacaggctcg cctattgtct
     4681 tctgaagcct cctcccctat gctagaaaac caattgacat ggtcatcgtc gcttctgaca
     4741 gcccactccc agggagcgca acggctagat ctgcagctga aattgaacca cccgccccat
     4801 gagctcgacc aactggcagg acctcacgga ttcagacttg ctccaatcct gcatcccgaa
     4861 taaccaggaa gcctttgcta tcctctatca acgatatgaa aaacgggtat tccattacct
     4921 catgacgatt ctgaatgaag cgacattagc tgaggaaacc ttagtggaag tcatgctggc
     4981 catctggaaa ggcctccaga cctttcaagg acaatcaaaa gtttccacct ggatttttgg
     5041 gattgcccat cataaggccg tggacgctct gcgcaaagtc accagccagc aaaggggcgg
     5101 cataccattg gaggacatc
//
LOCUS       DATIAL010000587         6269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_641791, whole genome shotgun sequence.
ACCESSION   DATIAL010000587 DATIAL010000000
VERSION     DATIAL010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641791"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VLA60_17705"
     CDS             <1..572
                     /locus_tag="VLA60_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11250.1"
                     /translation="LDRFRLRDEIETRINEHRDAERNSTFQTFLFADSPLTVSEERVI
                     NFKTIPYEPSWLYEGSFQFKKHYFGPKPGELNEITQSGTQTEEFQCAQFIDNLPEIKL
                     WVRNLVRKSSSFRLQTSKDWFYPDFICQLNDGRTLVVEYKGKHLYPDAEEKRAVGMVW
                     AARSQGKCLFVMPTETNFSTIVQSLKQPR"
     gene            641..952
                     /locus_tag="VLA60_17710"
     CDS             641..952
                     /locus_tag="VLA60_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HSF11251.1"
                     /translation="MKFIQDCFGRSVRLTNERVAHILQHKEMADMGTEIEQVLSAPAE
                     VRRSRSDATVQLFYEFYAKTRVGGKWLCVVVKYGTDDAFVVTAYLTDQLKDGAIVWPK
                     K"
     gene            937..1149
                     /locus_tag="VLA60_17715"
     CDS             937..1149
                     /locus_tag="VLA60_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HSF11252.1"
                     /translation="MAEKVKVWFDPEGDFLEVQFKDAPGFLRPTGHEAVMERVDEQGH
                     VLGFSIFGVSRFQKDHPLEAELVTDR"
     gene            1372..2151
                     /gene="rhuM"
                     /locus_tag="VLA60_17720"
     CDS             1372..2151
                     /gene="rhuM"
                     /locus_tag="VLA60_17720"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HSF11253.1"
                     /translation="MSTAGGEIVVYETPDGEIRVDVRLEQESVWLTQRQISDLFDTST
                     DNISLHLKNIFHEGELDESATAEDSSVVQMEGKRRVTRTIKHYNLDAIISVGYRVNSK
                     RGTQFRIWATQILRDHLIKGYSVNQRRLEELQRTVRLVATMAKHRDLSGNEATALLRM
                     VGEFSRALDLLDDYDHQRVPAPRTGSPTRYVLTYEEAIGIVNRLRERFRGSTLFGQEK
                     DESLHSSLQAIMQTFGGHDLYPSLEEKAAHLVFSGKKSLLC"
     gene            complement(2137..2316)
                     /locus_tag="VLA60_17725"
     CDS             complement(2137..2316)
                     /locus_tag="VLA60_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11254.1"
                     /translation="MRTNTSFSSVRFSAIIRVMATSAASEIKRAPAALVRTLVRSRNQ
                     RNNAAAIRSLPSTKE"
     gene            3091..>6269
                     /locus_tag="VLA60_17730"
     CDS             3091..>6269
                     /locus_tag="VLA60_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HSF11255.1"
                     /translation="MSPRIRDTGLETLWEEIRRFMGVCPSFFRLASPEPIVARGLFDL
                     AKFAYLESPLPSIFKEKLFTYLSRFCGVRYCVTRHAAFLLGRGNIAGDPTCPPLTPEE
                     VITLLSEPFPEGEQLSDILSALGTMRTPLKDWPANDSMVERHLRIACAIIFLEPGRST
                     AWCAELRRLLGSSQFEWLLLFLAFVRTAHFWTEVHASYDAMIPMASEHPSPGDDAVID
                     QTPQLVFEEDIEILFREQQALVEPLLQKMQDSTRFELGSRLHAELQELRTTHRLAQAL
                     KKSEALLRDLYENAPDMFCSVDAETERVVECNRTLLEKTGFSREEVIGTSVFERYTKD
                     SLPNARKSFEHFRQYGVEHNAELQLRTREEKTIDVSLNTTAVRDGSGRIISSRSIWRD
                     ISQQKSLEDQRRLIFEASPNGMLVVDQKGNILFANSRIEEWFGYPREELMGKPLEGLI
                     PAQFRVQQAGERFDFVLIPYVESMEPEPEFRGRGKDGRTIPLTIRLDPMGKEDEESTL
                     VTMTDITERQRDREIIRQSTIALHEQLALNKTITDNTSTSLLMIDTAGRVTFANPSTE
                     IVMGFKPEELIGRVLHEMVHHTRPNGSPFPIEECPLAGISSLRETLSNHEDVFIHKDG
                     HFYPVRCNTRQIFKQGERIGNVIEVLDITDLQRAEKDRQLFTLELARQVNDRTEELVI
                     SQKLLRELAAALNLTEQRERQRIATELHDYLSQLLVVCRLKLGQIRPASGMDSESSDL
                     IKETEDVLDQALTYTRTLVAELSPSVLFEFGLLAALQWLAGQMVHHGLKVEMTCEGIQ
                     DWKVPEEQAILLYQSIRELFLNVIKHADTDRAILTLSKEEGALRISVRDEGKGFHPPK
                     GTATQPFTDQAMKFGLFSIRERMITLGGTFEIESLPDQGTTARLIVPFALKLEKGSTP
                     PRDSDGQYIDQPTSNQSLPEAGDLSKISTKNQIRILIVDDHVMVREGLRKMLESHEDL
                     ELVGEAGNGEEAIDLVERLQPTLVIMDVNMPQLNGIEATSRIKANHPDIIIIGLSVNA
                     GGENQRAMLKAGAVALLPKEAAVADLYQM"
BASE COUNT         1639 a         1623 c         1523 g         1484 t
ORIGIN      
        1 ccctggaccg ctttcgattg cgtgacgaga tcgaaactcg catcaatgaa cacagagatg
       61 ccgaacgcaa ttcaacattc cagacctttc tttttgcgga ttctcctctc acggttagcg
      121 aggagcgggt tattaacttt aaaactatcc cctacgaacc aagttggctg tatgaaggta
      181 gctttcagtt taaaaaacac tatttcggcc ctaaacccgg tgagctaaac gaaattaccc
      241 agagcggcac ccaaaccgag gaatttcaat gcgcccagtt catagataac cttccagaaa
      301 ttaagctttg ggtgcgaaac ctagtgcgca aatcatcctc ctttcggcta caaacatcaa
      361 aggattggtt ttaccctgac ttcatttgcc aactcaacga cggccgaacg cttgtggtgg
      421 aatataaagg aaagcattta tatcctgatg ctgaggaaaa gcgtgcggta ggcatggttt
      481 gggcagcacg aagccaaggc aaatgccttt tcgtcatgcc aacagagacg aatttctcaa
      541 ctatcgttca aagccttaaa caaccaaggt aataaaaaaa acgagctgcg ccttttcccc
      601 tctcgcacaa tcctaacaac taattctcag agaaaacctc gtgaaattca ttcaagattg
      661 tttcggacga agtgtgaggc tcacgaatga gcgtgtggcc cacattcttc aacacaaaga
      721 aatggccgac atggggacgg aaattgaaca ggtcctctca gctccggccg aagttcggcg
      781 ctctcgatca gacgccactg ttcaattatt ttacgaattt tatgccaaga cacgagtggg
      841 tgggaaatgg ctctgtgtcg tggtaaagta tggcacagac gatgcgtttg ttgtgacggc
      901 ctatttaacc gaccaactca aagacggagc aattgtatgg ccgaaaaagt aaaagtgtgg
      961 ttcgatccgg aaggggactt tttggaagtc caatttaaag acgcccctgg atttctgcgt
     1021 cctaccggtc atgaggcggt catggagcgc gtcgatgaac aaggccatgt attgggattt
     1081 agcattttcg gtgtcagccg ttttcaaaaa gatcatcccc tggaagctga actcgtcacg
     1141 gacagataac cggaccacgg gaattcatca atcctgttgt cacgttgtaa tctgcctttc
     1201 tcccctcaaa gctgttatct tcactaaggg tttcaaaaaa aataccgctc cttttccgca
     1261 aaaaccattt cgtgtgaatg aagcctgacc tgcacgacac cctgtcgaga tggtatattg
     1321 ggtcccaccc gaccaaccaa ctttcctact ccattctgag agactctacg catgagcacg
     1381 gcaggcggag aaattgtcgt ttatgagact ccggacggag aaatccgggt ggacgtgcga
     1441 cttgagcagg agtccgtctg gcttacgcaa cggcaaattt cagacctctt cgatacatcc
     1501 acggacaaca tcagcttaca tttgaagaac atctttcacg aaggtgaact ggacgaatcg
     1561 gcaactgccg aggattcctc ggtagttcaa atggaaggca aacgacgggt cactcgcacg
     1621 atcaagcact acaacctgga cgccattatc tccgtcggct accgtgttaa ttccaaacgc
     1681 ggcacacaat ttcgcatctg ggcgacacag atattgcgtg atcatctgat aaaaggctac
     1741 tccgtcaacc agcgtcggct ggaggaactc caacggacgg tccggctggt ggcgaccatg
     1801 gcgaaacaca gggatctttc cggcaatgag gccaccgcgc tcctccgcat ggtgggcgag
     1861 tttagccggg cgctggatct gcttgacgat tatgaccacc aacgggtgcc cgccccccgg
     1921 actgggagcc cgacccgtta cgtcttaacc tatgaggaag ccattggcat tgtgaatcgc
     1981 ttgcgagagc gatttagagg ctcgaccctc ttcggccagg aaaaagatga aagcctgcac
     2041 agcagccttc aagcgatcat gcaaacgttt ggtgggcacg acctctatcc aagcttggaa
     2101 gagaaagcgg ctcacttagt attttctggg aaaaaatcac tcctttgttg atggcaacga
     2161 gcgtatcgcg gctgcgttat ttctttggtt tctggaacgc accaacgtcc tgactaatgc
     2221 cgccggggcg cgtttgattt cggatgccgc actcgtggcc atgaccctga tgatcgcgga
     2281 aaaccgcacg gaagaaaagg acgtgttggt gcgcatcgtc atgcatctcc tttgtcaaca
     2341 caatctattt tgaacaccgc cacagcaaac actgtctgct tcgattgcca ctctagactt
     2401 cagacagcga tgctagatgg atttggctgg actgttccaa ctttttattc tctcgttctg
     2461 aaaggaattc ataaccatga atctgacacc catcatgatc ggagtttctt tgagtgttgt
     2521 gctccacgca gggaaaagtc tggagcttgg ctggacactc atttgaaaat ttgtcgggta
     2581 agtgcgtgcc tcattccata tacaaaattt ggctggtggc tccttttaca tcttactgtc
     2641 tcgtgacttc ccttggccaa aacgtgttgg gcagggacca cggtcaccct catttaccta
     2701 tatcactgga agtgataagg gagctcaatc ttacattagg acccagctgg tggcttaaca
     2761 agcacatccc ctctcccaaa acttcttttg atacatcact tccttacctc aaatgtgaaa
     2821 agtgtctcaa ctcagtacca ctacaaaatg ggctaaaaaa atctgactcc gatcttttct
     2881 gaccctggca gcgagtgcat cccctgaaaa ttctaatgac accagcccct tatcgataca
     2941 attcgatctc cataattttg aagatgagta ccctgggatg gattcggatg tgatggatgt
     3001 ggcagcttgg ctttgtccga tcccctccaa agcttgtata atccgactag aagacgcccg
     3061 ataccatttc acgaaaatta ggaataaaag ttgagcccta ggattcggga taccggctta
     3121 gaaactctgt gggaagaaat ccgccgattc atgggagtct gtccgtcctt tttccggttg
     3181 gccagccctg agccgatcgt cgcccgcggc cttttcgatc tggcaaaatt cgcctatctt
     3241 gaaagccctc ttccctcaat tttcaaggaa aagctcttca cctatctttc ccgattctgc
     3301 ggggttcggt attgcgtcac acgacatgcg gcatttttgt tgggacgagg gaacatcgcc
     3361 ggcgacccaa cctgtcctcc tcttacaccc gaagaagtca tcaccttgct cagcgagccg
     3421 tttcccgagg gcgaacaact ttccgacatt ctgtccgccc ttggaaccat gaggactccc
     3481 ctcaaggact ggccggctaa cgattcgatg gtggagcgac acctgcgcat cgcctgtgcg
     3541 ataatttttc tggagcccgg gcgctccacc gcatggtgcg ctgaacttcg gcgacttttg
     3601 ggatcttcgc aattcgaatg gcttctgctt ttcctcgcct ttgtgcggac cgcgcatttt
     3661 tggacagaag ttcatgcatc gtatgatgcc atgataccaa tggcgagtga acacccttcc
     3721 cccggggatg atgccgtcat cgatcagact cctcaactgg tgttcgaaga ggacatagaa
     3781 attctctttc gagaacagca agcactggtc gaacctctct tgcagaaaat gcaggactcc
     3841 acacggttcg aactcggatc acggctccat gcagagttgc aggaattacg caccactcac
     3901 cggctcgcac aagccttgaa aaaaagtgaa gccctccttc gcgacttgta cgagaatgcc
     3961 ccggatatgt tttgttccgt tgatgccgag acagagcgcg ttgttgaatg caatcggacc
     4021 cttctggaaa agaccgggtt ttctcgcgag gaggtcatcg gcacatccgt tttcgaacgg
     4081 tataccaagg actccctccc aaacgcccgg aaatcctttg agcacttccg ccaatatgga
     4141 gtcgagcaca atgccgaatt gcaattgcgg actcgtgaag aaaaaacgat tgatgtcagc
     4201 ctgaatacca cggccgtgcg tgatgggtcg ggtcgaatta tctcaagccg ctcgatttgg
     4261 cgggatatca gccaacagaa atcccttgaa gaccaacggc gcctcatatt tgaagcctcc
     4321 ccgaatggca tgctggttgt ggaccagaag ggaaacattc ttttcgccaa ttcacgaata
     4381 gaggaatggt tcggttatcc ccgcgaggag ttaatgggaa agccattaga aggtcttatt
     4441 cccgcgcaat ttcgagtcca gcaggccggc gagcgttttg acttcgtgtt gattccctac
     4501 gtcgaatcca tggaacccga gcctgagttt cgtgggcgtg gtaaagacgg aaggaccatc
     4561 ccactcacaa tcaggctcga tccgatgggg aaggaagacg aagaatcgac attggtgacg
     4621 atgacagata tcaccgaacg gcaacgggat agggaaataa ttcgccagtc gaccatcgcg
     4681 cttcatgaac aattggctct caacaaaacc atcaccgata atacctcaac aagcttgctt
     4741 atgattgata ccgccggacg agtgaccttt gccaatccat ccaccgagat cgtgatgggg
     4801 ttcaaacctg aagagctgat tggccgggtt ttgcatgaga tggtccacca tacccgtcca
     4861 aatggatccc catttccaat agaagaatgt cccctggctg gtatatcttc cttaagagag
     4921 actctgtcca atcatgaaga cgtatttatc cataaagatg gccattttta tcctgtacgc
     4981 tgcaacacca ggcagatttt caagcaaggg gagcggatag ggaacgtcat cgaagttctg
     5041 gatatcacag atctgcaacg agccgagaaa gaccgtcaac tttttacgct ggaacttgcg
     5101 agacaggtca atgatcgaac cgaagagctc gtcatttctc aaaaactgtt acgggaatta
     5161 gcggccgccc tcaatttgac cgaacaacgg gaacggcaac gtattgcgac ggaacttcat
     5221 gattacctca gccaactatt agtggtgtgc cgattgaaac tgggccagat cagaccggcc
     5281 tccggcatgg actctgagag ttccgatctg atcaaagaaa ccgaagacgt cctcgatcaa
     5341 gccctcacct acaccagaac gctcgtcgcg gaattaagcc cttcggtctt atttgaattt
     5401 ggattgttgg cggccttgca atggctggcc ggtcaaatgg tccatcatgg gctgaaagtg
     5461 gaaatgacct gtgagggcat tcaagactgg aaggtcccgg aggaacaggc gattttgttg
     5521 tatcaatcaa tccgcgaact gtttctcaac gtgataaaac atgccgacac ggaccgtgcc
     5581 atactcacgc ttagcaaaga ggagggggca ttacgaattt ccgtccgcga tgagggtaag
     5641 gggtttcatc cccccaaagg aactgccaca cagcctttca cggaccaagc gatgaaattt
     5701 ggcctgttct ccattcgcga acgcatgatt acccttggag gaacattcga aattgaatca
     5761 ctacctgatc agggaaccac ggcccgcctc attgtaccct ttgcgttaaa gttggaaaag
     5821 ggttcaacac caccacggga ttcggacggg cagtacatcg atcaacccac gagcaatcaa
     5881 tctctaccgg aggcaggcga tctctcaaaa atcagcacaa aaaaccaaat tcgcattctc
     5941 atcgtcgatg accatgtgat ggtgcgagag ggattaagga aaatgttgga gagccatgaa
     6001 gatctggaac tggtaggaga agccgggaac ggcgaggaag ccatcgattt ggtggaacgc
     6061 ctgcaaccta ccctggtgat tatggatgtc aatatgccgc aactgaatgg tattgaggcc
     6121 acatcccgaa taaaagcgaa tcatcccgac atcattatta tcggattatc cgtcaatgcc
     6181 ggtggagaaa accaacgagc aatgctcaag gccggagccg tcgccctctt gccaaaagaa
     6241 gctgcggtcg ccgatctcta tcaaatgat
//
LOCUS       DATIAL010000588         5329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_749278, whole genome shotgun sequence.
ACCESSION   DATIAL010000588 DATIAL010000000
VERSION     DATIAL010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749278"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VLA60_17735"
     CDS             <1..389
                     /locus_tag="VLA60_17735"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HSF11256.1"
                     /translation="LMVLALFRFILQRACASPHAVVEPWLGTRRLEYEDLSRALLLTV
                     LFAGVIFISWILFLAGGFDPLDALFEVVSATGTVGLSTGITTPELPGWLKGVLCFDML
                     AGRMEIIALLMMVYPGTWRERRTRAQ"
     gene            386..1093
                     /locus_tag="VLA60_17740"
     CDS             386..1093
                     /locus_tag="VLA60_17740"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HSF11257.1"
                     /translation="MRAIFIGASSLTVMTAEILLKRGHEVVIVESNKERINELSKDLD
                     CGFVHGDGTTPAIQRETDPEHSDFLFCLAGNDQANIIASLVGRSLGFPRVVTKIVNPE
                     FEHICVELGLEETIIPAGTMGRYLADKFAGRDLLELSGMIKDEARVFSFVARPEDEGP
                     IEKIAIPGQLHPICLYRDHTFILPDTDTEVKAEDEVVVITHQKLLAQLKDRWAVKKES
                     HQSSLSGGQDNPDEIPT"
     gene            1527..2279
                     /locus_tag="VLA60_17745"
     CDS             1527..2279
                     /locus_tag="VLA60_17745"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HSF11258.1"
                     /translation="MKNIGRIHIGTSGWHYPHWVGPFYPSGMASEEFLPFYARHFGTV
                     EINNTFYHLPALHTLREWEKETPHNFLFTCKGSRFITHMKKLKDPEHSIARFFETITV
                     LRHKLGPILFQLPPRWRMNVLRLEEFLKALPKTFRYAFEFRDESWFAQPVYDLLTKYD
                     SAFCLYHLAGRWSPEIITTDFVYIRLHGPGEAYQGKYRAPTLRLWANKCRKWAQSGKD
                     VYCYFDNDQDGYASINALTLQKNVQALLKKSC"
     gene            2358..2717
                     /locus_tag="VLA60_17750"
     CDS             2358..2717
                     /locus_tag="VLA60_17750"
                     /inference="COORDINATES: protein motif:HMM:NF013541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009374 - biotin binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="avidin/streptavidin family protein"
                     /protein_id="HSF11259.1"
                     /translation="MAEEPFVNFEGTWKNQHQSILELTVVEGLVRGRFQSGVGEDGQL
                     LWAEINGRILGDVITFHAAYPLYRTIVTWIGQHLVENGIGSIHTHWLHATDIKEREEH
                     EWMWYSNRIGSDVFTRV"
     gene            complement(2813..3058)
                     /locus_tag="VLA60_17755"
     CDS             complement(2813..3058)
                     /locus_tag="VLA60_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11260.1"
                     /translation="MELVAGDILEGQVEYQTSDGWKIVVFSDGDVWDYVRAMTPPTKE
                     HFEIWPEQEKDDCERFRKILAYHPPTAQLKTTWGFLC"
     gene            3339..4625
                     /locus_tag="VLA60_17760"
     CDS             3339..4625
                     /locus_tag="VLA60_17760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HSF11261.1"
                     /translation="MPSSTTFSKSTLFAISYLLLTVLFFKLGDGLQGILVPIRAQMEG
                     FSLPSIGLLGTAFYSGLVIGCLFVPTLIQRVGHIRVFSGFAALAATAFLLHDFVTTVP
                     VWLGLRMAIGFCFAGLYMAIESWLNDQATTHTRGRILGTYMLVSWVAVIGGKLLFAFM
                     DPVGVFPFALVSMGICLAVVPVAFGTGPAPAPATEKPLRLRKLYRISPVGMVGSLSIG
                     ITNGAFWALAPIYAQSRGLAPLQIGLFMSAAVLGGALTQWPLGRWSDWIDRRRIIAGS
                     CLTAALAAGALAMADQSHELIILILALVFGAGALPLYALCLAHANDFAPPESFIQVSR
                     DLLMVFGFGAIVGPYLGAWFMTIGGPLGLFLFTGSIHLALTAYTLIRIWQRPPVPETE
                     KTAFVSIPRTTQAILTLDPRSEETGRMASTQQIKGE"
     gene            4715..>5329
                     /locus_tag="VLA60_17765"
     CDS             4715..>5329
                     /locus_tag="VLA60_17765"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HSF11262.1"
                     /translation="MPVHNTEIAEVFSRLAELLEIQGANPFRIRAYRKAAQTIEGLPH
                     SVASMLAEGSDLSDLPGIGQDLAAKIQEIVETGHLSLLDQVSSQLPGQLAELAKIPTL
                     GPKRVKMLYDTLGIKDLKGLAKAARAGKIRELHGFGPKTEEKILAETDKRSGTEQRIK
                     ISTAEEFAEPILDYLKSAKGVKDAIVAGSYRRRKDTIGDLDILVT"
BASE COUNT         1321 a         1400 c         1353 g         1255 t
ORIGIN      
        1 tcctgatggt gctcgcatta ttccgtttta tcttacagcg ggcctgcgcc tcgcctcacg
       61 ccgtggttga accatggctg ggcaccaggc gtttggaata cgaagacctc agccgggccc
      121 tattgctcac cgtactgttc gcaggtgtaa ttttcatatc ctggatacta ttcctggctg
      181 gaggattcga ccctctggac gctctctttg aagtagtatc ggcgactgga acagttggcc
      241 tctctaccgg catcaccacc ccagaactgc ctggatggct gaaaggtgtc ctctgttttg
      301 atatgctggc cggacgcatg gaaatcatcg ccctgcttat gatggtgtat cctggaacct
      361 ggcgtgaaag gaggacacga gcacaatgag agcgatattt ataggagcca gctcgttgac
      421 ggtgatgacc gcggaaattc tcttaaagcg agggcacgaa gttgtcatcg tcgaaagcaa
      481 caaagaacgg atcaatgaac tcagtaagga cctcgattgc ggattcgtcc atggggacgg
      541 aaccacgccg gccattcaac gagagacaga ccccgagcat tcagattttc tgttttgttt
      601 agccggcaat gatcaagcca acattattgc cagtttagtc gggcggtccc tgggtttccc
      661 gcgcgtggtc accaagattg tcaatccgga atttgaacac atctgtgtgg agctgggcct
      721 tgaagaaaca atcattccgg ccggcaccat gggacggtat ttagccgaca agttcgccgg
      781 ccgggatttg ctcgaactgt cagggatgat taaagatgag gcccgtgtct tttcctttgt
      841 ggcccgtccg gaagacgaag gccccataga aaagattgct atcccggggc aacttcaccc
      901 catctgtctc taccgggatc atacgttcat cctgcccgat acggacacgg aagtgaaggc
      961 agaggacgaa gtggtggtaa tcactcacca gaaacttctg gctcaactca aagatcgttg
     1021 ggccgtgaag aaagaatcgc accaatcctc tctttccggt ggccaggata atccggatga
     1081 aatccccacc taagttctga aaacctcacg acaggtatgt aaaaatctga gcgtcatgtg
     1141 tcagacaata tcagatccat ggtgaacagg ttgggtgttg aacaatgaag gccttccgtc
     1201 aaaccggcat tgctcatcca agagtgaaaa gggaggagtg aggggtaagg aatgaggagg
     1261 aggaagagag aggatgtccc gttggctacc ggcggggatg acgtgaaacg tgagaaatga
     1321 ggagcaataa accttcaggg gccgggagtg aggggaaatg ctccaggtcg ttctcgctta
     1381 gacaaagggg gataaaggcg gatttttcgc ttgaaggact gcgaaaaaaa cacactgaac
     1441 gagtactcac aacaccatgc acacacaacg gctcaaaact tgaggctatg catgttgagt
     1501 gttgtccctt tatgacctga atacccatga agaacatcgg cagaatccat atcgggacgt
     1561 caggctggca ttatcctcat tgggtgggac ctttttatcc gtccggcatg gcgagtgaag
     1621 agtttctccc gttctatgcc cgacacttcg gcactgttga aatcaataac acattttatc
     1681 atcttccggc tcttcacacc ctgagagagt gggaaaaaga aacaccccac aacttcctat
     1741 tcacctgtaa gggcagccgt tttatcaccc acatgaaaaa gctgaaagat ccggaacaca
     1801 gcattgcacg attctttgag accatcaccg tgctgcgcca caaactggga ccaatcctgt
     1861 ttcagttgcc accacgttgg agaatgaatg tgctcaggct ggaagaattc ttgaaggccc
     1921 taccaaaaac attccggtat gctttcgaat ttcgtgacga aagctggttc gcacaacccg
     1981 tctatgacct gcttaccaaa tatgatagcg ccttttgtct ctatcacctt gccggtcgat
     2041 ggtcaccgga aatcatcacc acggattttg tgtatatccg gcttcatggc cccggcgaag
     2101 cctatcaagg aaagtatcgt gccccaactc ttcgcctttg ggccaacaaa tgtcgcaagt
     2161 gggcccaatc cggcaaagat gtttactgct atttcgacaa tgaccaggac gggtatgctt
     2221 cgatcaacgc gctcacccta caaaagaacg tacaagcgtt actgaagaaa tcatgttagg
     2281 gaaacaggac cggtaatgga gaataaccat gcggaaacct gtcaaggaac caaccgcact
     2341 gaaccgagga gaagatcatg gccgaagaac cttttgtcaa cttcgaagga acctggaaaa
     2401 accaacatca atccatttta gaattgacgg tggtggaagg attggtcaga ggacgatttc
     2461 aatcaggggt cggagaggat gggcaactac tctgggcaga gatcaacgga cgcattcttg
     2521 gggatgtcat tacctttcat gcggcgtatc cactctaccg caccatcgtc acctggattg
     2581 gacaacacct ggtggaaaac ggaatcggga gtattcatac ccactggctg cacgcgacgg
     2641 acattaagga gcgagaggaa cacgaatgga tgtggtactc caatcgcatt ggttccgatg
     2701 tcttcacgag agtctgaact atcctgtagg agaaaaccaa ctcattcaaa aaacccacga
     2761 aatcggcttg atcctggtct gtgctgtctt ggggaccatc ccgaacatcg gatcagcaca
     2821 aaaaccccca tgtggtcttg agttgagccg taggaggatg gtaggccagg atttttcgaa
     2881 acctctcaca atcatctttt tcctgttcag gccaaatctc aaaatgctcc ttggtaggag
     2941 gagtcatcgc tcgcacataa tcccacacgt ctccatcact aaagaccacg atcttccatc
     3001 catcgctcgt ctgatattca acctggccct ctaaaatatc tccggcgact aattccacgc
     3061 tggcctcccc acgttcaata cgttggagtt cattgaatgc ggcttcgaaa agtcgtctct
     3121 ggtcattgaa ctctctttat cgataggata actatagcaa cagtgttcat gaagtcttgc
     3181 cctttggggt cggttcaaca acgactgctc ccaaccaagg aaaggctcac agggaaaacc
     3241 ctgtcccccc aagaatcctg tatgaaatag ttttatcaag aggcaagtcg acagaaccat
     3301 tcccacaact ttttatacga tcgtgatgca cacacaacat gccttcctcc accaccttca
     3361 gcaaatccac cttgtttgcg atttcatatc tcctgctgac ggtgctcttt tttaaattag
     3421 gggatgggct gcaaggcatc cttgttccga ttcgggccca gatggaaggc ttctcgctcc
     3481 cttccatcgg tttactggga accgcattct attcgggcct cgtcatcggt tgcctatttg
     3541 tcccaactct catccagcga gtcggacata ttagagtgtt ctcaggattc gcggcactgg
     3601 cagccacggc atttcttctt catgatttcg tgaccaccgt cccggtctgg ctcgggttac
     3661 ggatggccat tggattttgt tttgccggcc tctacatggc tattgaaagc tggttgaacg
     3721 accaggccac aacccacacg cgcggacgca tcctcggcac ctatatgctt gtcagctggg
     3781 ttgccgtcat tggcggtaag ttgctcttcg ccttcatgga tcccgtcgga gtatttccct
     3841 tcgcattggt cagcatggga atctgtctcg cggttgttcc ggtagcattt ggaacgggtc
     3901 cggcacccgc accagccacg gaaaagcccc ttcgcctacg caaactctac cggatatctc
     3961 ctgttggtat ggtcggttct ttgagtatcg gcatcacgaa tggggccttt tgggcgttgg
     4021 cgccaatcta tgcgcaatcc cgtggccttg cccctctcca aatcggccta tttatgagtg
     4081 cggcagtttt gggcggggcg ctcactcaat ggccgttggg acgctggtcg gattggatcg
     4141 atcgtcgccg gatcattgcg ggatcatgtc tcacggcagc actggcagca ggagccttag
     4201 ccatggcaga ccagagccat gaactcatca ttctgatcct tgcccttgtg tttggggcgg
     4261 gggccctccc tctctacgcg ctctgtcttg cccatgccaa cgactttgcg cctcccgaaa
     4321 gcttcattca agtcagccgc gatttgctga tggtatttgg atttggagct attgtcggtc
     4381 cctatctcgg ggcatggttc atgaccatag gcggacctct tggcctcttt ctcttcacag
     4441 ggagtattca tttggccttg accgcgtaca cactgatacg catttggcaa cggccgccgg
     4501 taccggaaac agagaaaacg gcttttgtca gtattccaag gactactcaa gccattttga
     4561 cgcttgatcc ccgttcggag gagacaggtc gtatggcctc cacccaacag atcaaggggg
     4621 aataatgtcg gtctcgcgac ctgtcacaac ctgatagcga ggaacatgta ctcttcacaa
     4681 atagacatgt catcatagga gacggtccgc agccatgcca gtgcataata ccgagattgc
     4741 cgaagtgttc agccgtctgg ctgagctttt ggaaatccaa ggcgccaatc ccttccgcat
     4801 tcgagcctat cgaaaggccg cgcagacgat cgaaggccta ccacacagtg tagcatcgat
     4861 gttagccgaa ggttcggatc tctcagacct tcctggtatc ggacaagatt tagcagccaa
     4921 gatccaagag attgtggaaa cgggtcatct gtcactctta gaccaggtca gttcccaact
     4981 acccggtcaa cttgcggaac tagccaagat cccaactctt ggccctaagc gggtgaaaat
     5041 gctctacgac acactcggca tcaaggacct gaagggtctg gcaaaggcag cccgagccgg
     5101 gaaaatccgg gagcttcacg gctttggacc aaaaaccgaa gaaaaaattc ttgcagagac
     5161 ggacaagcgc tcaggcacag agcagcggat caagatcagt acagccgaag agtttgcgga
     5221 gccgatcctc gactatctga aaagcgcgaa gggagtgaaa gacgccattg ttgccggcag
     5281 ctatcgccgg agaaaagaca cgattggcga ccttgatatt ctggtgaca
//
LOCUS       DATIAL010000589         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1503487, whole genome shotgun sequence.
ACCESSION   DATIAL010000589 DATIAL010000000
VERSION     DATIAL010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503487"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VLA60_17770"
     CDS             complement(<1..691)
                     /locus_tag="VLA60_17770"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HSF11263.1"
                     /translation="MKTHPSGQLQQLGTWQTVAYLFRHTTSGEGRWILAGVLTLLASS
                     AIALLQPWPLKLVVDAILGSQEPPVFLTDLNGLLSQHFFWLSDTKTGLLLLLCLCVLV
                     IQLLMGTLMVLSTYILVAVGLRMVFRLRCRLFDHLQRLSLAFHDKTAVGDSLYRMTWD
                     TYCVQALFNSGVIPALTASFTLLGIAGIMFFLDWLLTMVALAIGIPLVILIRKLERPM
                     QERTMRAAEGES"
     gene            complement(731..>2054)
                     /locus_tag="VLA60_17775"
     CDS             complement(731..>2054)
                     /locus_tag="VLA60_17775"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HSF11264.1"
                     /translation="GIGGPNLPPPDDGWIAECVANAPGGPIHVLLDDQTAEHIPGCNM
                     SFRKTCLESIGGFDPQFRTAGDDVDVCWRLQEKGWTLGFHPSALVWHHRRNSVKTYWK
                     QQRGYGRAEALLEKKWPQKYNAAGHPSWLGRLYGRGSTSLPSWRPSRIYQGVWGCAPF
                     QLLYHAHPSTTLSLLSMPEWNLALGFLAILSLLGIFWSPLLWALPFLIVSLGGALFLA
                     ARNAHKGTFEVPKTPLGQVRSKIVTAGLHLAQPLARLTGRLCSGLTPWRQQVSEKFVF
                     PWSRTLTTWSEQWRGVSDRLECLERKLQAQRVIVHRGGDYDRWDLHIKTGLFGGTRAR
                     MAIEEHGAGKQLIRCRVWPRFSIIAMTGMMVFGLLGIWAYLDQAWVVGTILGGFSLLL
                     GVRAHQEYSFAIISVHDALLSELVVQPVEQPKEVEEKFLLPHSTPILD"
BASE COUNT          544 a          543 c          523 g          444 t
ORIGIN      
        1 tgctctcacc ttcggccgct ctcatagttc gttcctgcat cggtctctcg agttttcgaa
       61 ttaaaatcac gagaggaatc ccgatagcca gcgccaccat cgtgagcaac cagtcaagaa
      121 aaaacatgat gccggcgatg cccagcagcg tgaacgaggc agtcagcgcg ggaatgactc
      181 cgctgttaaa caaagcttga acgcagtagg tatcccaggt catccggtac agagagtccc
      241 cgacggctgt cttgtcatga aacgccagag ataatctctg cagatggtca aagagccggc
      301 atcgaagtct gaacaccatt cgcagtccta cggcgaccag gatataggtg ctgagaacca
      361 tgagagtgcc catgagcagt tggatcacca ggacacacag gcataagagg agaaggagac
      421 ccgtcttggt gtctgaaagc cagaaaaagt gctgggacaa caacccgttt aagtcagtca
      481 gaaaaaccgg cggttcctgg cttcctaaga ttgcgtccac gaccagcttt aagggccacg
      541 gttgcaacaa agcgatggct gaagaagcta ataacgtcaa aactcctgcc aatatccacc
      601 gtccctcccc ggatgtggtg tgacggaaca ggtaggcaac cgtttgccac gttcctaatt
      661 gttggagttg gcctgaagga tgtgttttca tggaggcttt ttatggcggg gaaaggggaa
      721 aatctttgcc ttaatccaga atgggggtag agtggggtag aagaaacttc tcttccactt
      781 ctttcggttg ttcaacgggc tgaacgacca gttcgcttag aagtgcatcg tgaacggata
      841 ttatagcaaa ggaatactct tgatgtgcgc gaactcccaa taggagagaa aatcctccaa
      901 gaatggttcc gactacccag gcctggtcaa gataggccca gatacctaat agtccaaaaa
      961 ccatcattcc tgtcatagca attatggaaa accggggcca cacccggcac cggatcagtt
     1021 gtttccccgc gccatgctct tcaatcgcca ttcttgcacg ggttcctcca aacagaccgg
     1081 ttttaatatg gagatcccac cggtcatagt cgccccctcg atgaacgatg acccgctggg
     1141 cttgaagttt tcgttctaaa cactctaatc gatccgacac gccacgccat tgttcactcc
     1201 aggtcgtcag ggtgcgagac cagggaaata cgaatttttc cgaaacttgc tgacgccagg
     1261 gggttaagcc ggaacataga cgtccggtta aacgtgcaag gggctgggca agatgaagcc
     1321 cggcggttac gatcttggac cggacttgac cgagaggggt tttgggtacc tcaaacgtgc
     1381 ccttgtgagc attccgtgct gccaggaaca atgctccacc caacgagacg atcagaaaag
     1441 ggagtgccca cagcaatggc gaccagaaga ttccgagaag ggacagtatt gccaggaatc
     1501 ccaaggctaa attccactcc ggcatactta acaaggacaa ggtcgttgaa ggatgtgcgt
     1561 ggtacaggag ttgaaacggc gcacatcccc atacaccttg ataaattctt gatggacgcc
     1621 aggatggcaa cgacgtcgat cctcgtccat acaatcgacc cagccatgaa ggatggccgg
     1681 ccgcattgta tttttggggc cacttttttt ccaatagcgc ttcggcccga ccatagcccc
     1741 gttgttgttt ccaatacgtc ttgaccgaat tccgccgatg atgccacacc agagccgatg
     1801 gatggaatcc caatgtccac cccttttctt gcaatcgcca acagacatcc acatcgtctc
     1861 cggccgttcg aaactgggga tcaaagcctc cgatcgattc aagacatgtc ttgcgaaaag
     1921 acatattaca accaggaata tgttccgcag tttgatcatc gagtaacaca tgaatgggac
     1981 ctccgggagc attggcaaca cattcagcaa tccagccgtc atccggggga ggtaaattcg
     2041 gtccaccgat tcct
//
LOCUS       DATIAL010000590         7077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_105280, whole genome shotgun sequence.
ACCESSION   DATIAL010000590 DATIAL010000000
VERSION     DATIAL010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105280"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VLA60_17780"
     CDS             complement(<1..374)
                     /locus_tag="VLA60_17780"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HSF11265.1"
                     /translation="MSYFESIQSLIQDFSKGNPLGLKLSEVQLQQEWEHLVGATMAKH
                     SYPESIRFKKLHLVADNSIWLQQLMFLRPAILEAIHSMMPELGLTEVVLRIGSIPQAP
                     PQPDPLPQDPPDFVGEPSPFATG"
     gene            538..1017
                     /gene="smpB"
                     /locus_tag="VLA60_17785"
     CDS             538..1017
                     /gene="smpB"
                     /locus_tag="VLA60_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HSF11266.1"
                     /translation="MVKQKTDSSNKVLSTNRKAFYDYMIEEKVEAGIVLVGTEVKALR
                     EGRLNLKESYAAIINGKAILHNCHIGTYSHGNQMNHEPLRPRTLLLHKKEIERLAEKV
                     QQKGLTLIPLRLYFNDRGRVKLEIALGRGKKNYDRRDTIKKREAGREIDRAMKESRS"
     gene            1297..1782
                     /locus_tag="VLA60_17790"
     CDS             1297..1782
                     /locus_tag="VLA60_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HSF11267.1"
                     /translation="MRRTQVSGGRANSLCSNRARQFLGAFVSKPGRQASFKGEKGCLE
                     GFREPPIAFKYVLGVLWAFTVLTAANNGVSPTNKISEIIWKDHIQQSRTRKEIKRLTE
                     KVQQKGLTLVPLRLYFNDRGRVKLELALGRGEKNHDRRDTIKKREAGREIDRAMKESR
                     S"
     gene            complement(1930..2898)
                     /locus_tag="VLA60_17795"
     CDS             complement(1930..2898)
                     /locus_tag="VLA60_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11268.1"
                     /translation="MNTILKNAVQSIQIGIEDYQSSSDDHRRVLSAVRNITAGILLLF
                     KEKLRQLSPEKSHEVLLYQDIRPKLDQEGNISFQRAGRKTVNFNQICERFKDLGIPIE
                     HKLLKGIVDIRNGIEHFFTTETPSRLRELLADSFIILRDFITTQLNENPVDLLGNDTW
                     EVFLDVAAVYNKELEDCQTECDKIIWKEPALSSIGGFLRCFSCHSELIRPINPEVNDQ
                     TTLKFSCRACGELSQFEDIAEAACAEAYEWQDYVAMTDGGEPPVTTCSECGRESYVVE
                     EDFCVTCGTPGRNYAECSICHADLSMEEQDFGGLCSYHQYQMEKDD"
     gene            complement(3048..3311)
                     /locus_tag="VLA60_17800"
     CDS             complement(3048..3311)
                     /locus_tag="VLA60_17800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HSF11269.1"
                     /translation="MKELKIKMKPSHPGAFIKSEVIDELGVSVTKAAEVLGVRRATLS
                     DLVNEKAALSAEMALRVEKAFQLNMDMLLRMQAWYDATEMRKY"
     gene            complement(3334..3939)
                     /locus_tag="VLA60_17805"
     CDS             complement(3334..3939)
                     /locus_tag="VLA60_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11270.1"
                     /translation="MSDEDLLSRGKDFVQKLQEALVASTFALVFQEHISPVQIRMAKV
                     HEKLLDFELISQENSAFQFELTTAYPPDYKIREQYKNGRRPSFPMQTLSGTPMNPEWI
                     APVICKKTEKARRIDGFNGHLLVYVNIHGGGPVGRGLEELMDLISDSETIWQSIWLIR
                     GIPDLVSTGTVLLCNSSGLGCEEREWLHIKDNNGLKRLVDE"
     gene            4519..5430
                     /locus_tag="VLA60_17810"
     CDS             4519..5430
                     /locus_tag="VLA60_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11271.1"
                     /translation="MSNPTDQLSSPDPAAPVFRPGDFKGREGLVRNMLRRLERGESLS
                     LVGGPKLGKTSMLLHLAWQLNHVGPSPKSTGPSALYVDVADEADWKRFHSRPPNPETV
                     LLLDNCDRLVEGKTRSLSDIDLLPGGSTVFAGGRAWREVVREGNLPHTLKLIPLSVFL
                     EKEAQQLFSRDLSAEQQATVLTYAGTHPYIFKLFQAAFLHKEIHIPTEQIVSEVKKSL
                     SFFFQDCINQLREPLEHQVLTFVVEANKPVNPREVARAVGLPTIKPVADTLCALGLVA
                     RWIRDEEATLSASSRMFNEWYRETVIS"
     gene            5516..6127
                     /gene="yihA"
                     /locus_tag="VLA60_17815"
     CDS             5516..6127
                     /gene="yihA"
                     /locus_tag="VLA60_17815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HSF11272.1"
                     /translation="MGAFKIFSAEFKASCGTVEQFLKPTLPEVAIVGRSNVGKSSAIN
                     CLVNQKGLAKVGKTPGKTQTINFFEIATNGPRFMLVDLPGYGFAKVPERIRELWGPLI
                     EEYLRTRKNLQGVVVLVDSRRVQESDRAMVEWLMRLKIPVMVVATKADKVTRGHRQAA
                     LRELREGLGMEEDPLFLSAYTGEGKQLLLDQLREVLTLDAPLP"
     gene            6628..>7077
                     /locus_tag="VLA60_17820"
     CDS             6628..>7077
                     /locus_tag="VLA60_17820"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HSF11273.1"
                     /translation="MPLFGDTNTPMTFNPPSRLDRIGPSQIRENMRIAMEHEAINLAQ
                     GRPDFPTAPVVKQAAIDAIANDHNQYSVTWGLAELREAIARHVHERHGLTFDPETEIT
                     ITCGVTEAIVAAMLALVESGDEVIIIEPAHENYVPAVYFASGTPRFVT"
BASE COUNT         1837 a         1736 c         1718 g         1786 t
ORIGIN      
        1 ccggtggcaa agggcgacgg ttcaccgaca aaatcaggag ggtcctgggg tagtggatcc
       61 ggttgaggcg gggcttgtgg aatggagccg atgcgtaaaa cgacctcggt taagcccagt
      121 tccggcatca ttgaatgaat ggcttccaaa atggccggcc ttagaaacat gagctgctga
      181 agccaaatcg agttatccgc taccagatgc aattttttaa agcggatgct ctcagggtag
      241 gaatgtttgg ccatggtcgc acccacaaga tgctcccact cctgttggag ttgaacctca
      301 gacaatttga gaccgagagg attacctttg gaaaaatctt gaataagact ctggatcgac
      361 tcaaaataac tcatcgtgaa aacattgtac cgtattgcac ggagtgattc atccgcctaa
      421 gactgacacg tggtgtcttc ttgaccctag gaagagatta tttacacgag gcaggcaatg
      481 gaacgaaaaa gtaaaaatga cagccaaatc attccataca acaagatata attagctatg
      541 gtcaaacaaa aaacagactc ctccaataaa gtcctcagca ccaaccgcaa ggccttctat
      601 gactacatga tagaagagaa ggtcgaagct ggtatcgtcc tggtcgggac cgaagtcaaa
      661 gccttacggg agggccggct gaatctgaag gagagttacg ccgccatcat caatggcaaa
      721 gctattctgc ataactgcca tatcggcacc tacagccacg gcaatcaaat gaatcacgag
      781 ccgctacgtc ccagaactct gctattgcac aagaaagaga tcgagcggtt ggccgaaaaa
      841 gtccagcaga aaggactcac cctcatcccg ctccggctct atttcaatga cagagggcgg
      901 gtcaagttgg aaatcgccct ggggcgaggg aagaaaaact acgaccggcg ggacaccatc
      961 aaaaagcggg aagccggccg cgaaatcgac cgcgccatga aagagtcacg ctcctagaag
     1021 tctgtcgggt ttcctccgca atcggggcct tggaaaatga cggcagaacc cccttccttg
     1081 gaccttcctg aaaatatttc catatcggct cggctggtgt tacttccaaa agatgttggg
     1141 ccagggaatt gggcgtctat ttatagtcat tccaataaac ttccactctg tttaggagcg
     1201 aaaaaccaca agtgccgggt ttcggcccgg cagccgagcc acttttgttt cggcaaaagt
     1261 agccaaaacc agggacgccg cgtccggttt catcagatga gacgaacgca ggtctcggga
     1321 gggcgggcca actcgctgtg ctcaaacagg gcccgccagt ttctgggagc gttcgtctct
     1381 aagcccggtc ggcaggcgtc attcaaaggg gaaaaaggat gccttgaggg gtttagggag
     1441 ccccctatcg catttaagta tgtgctaggg gtgttgtggg cgttcaccgt tttaacagca
     1501 gcgaacaatg gtgtctcacc cacaaacaag atttcggaaa ttatttggaa agaccatatt
     1561 caacagtctc gcacaagaaa agagatcaag cgactgaccg aaaaagtcca acagaaagga
     1621 ctcaccctcg ttccacttcg cctctatttc aatgacagag ggcgggttaa gttggaactc
     1681 gccctagggc ggggggagaa aaaccatgac cggcgggaca ccatcaaaaa gcgggaagcc
     1741 ggccgcgaaa tcgaccgcgc catgaaagag tcgcgctcct aggaagttgt cgggtttccc
     1801 aggccttcct gaaaatatcg ccgtgtcgag ccagatggtt gtttcctaaa cgatgatagg
     1861 agtgggcaga agaaccaatt aatcagttaa tcttgttatt ttaggataaa aacccaggac
     1921 ttttgttaat taatcgtcct tttccatttg gtactgatga tacgaacaca aacctccgaa
     1981 atcttgctct tccatactaa gatcggcgtg acagattgaa cattccgcat agtttcttcc
     2041 aggggtgcca caggtgacgc aaaagtcttc ttctacaaca taagattcac gtccgcattc
     2101 agaacaagtg gtcacaggtg gttcccctcc gtcagtcata gccacataat cttgccattc
     2161 gtacgcctct gcacatgccg cctctgctat atcctcaaat tgagaaagct caccacacgc
     2221 acggcaagaa aacttaagtg tcgtctggtc atttacctcg gggttaattg gtcttattaa
     2281 ttccgaatgg caagagaaac aacgtaagaa tcctcctata ctggataggg ctggttcttt
     2341 ccaaatgatt ttgtcgcact cggtttgaca gtcttcgagt tctttattgt aaacagccgc
     2401 aacatcaaga aatacctccc atgtatcatt acccaaaagg tctacagggt tttcatttaa
     2461 ctgagtggtg ataaagtccc tcaaaataat aaatgaatct gcaagaagct ccctaagccg
     2521 cgaaggggtt tcagttgtaa aaaaatgttc aatgccgttc ctaatatcta ctataccctt
     2581 taagagcttg tgttcaatgg gaattccgag gtctttaaaa cgttcacaaa tctggttgaa
     2641 attaacagtt tttcttccgg cacgttgaaa tgaaatgttt ccttcctgat caagctttgg
     2701 tcggatgtcc tgataaagta aaacctcatg ggatttttca ggagaaagtt gtcgaagctt
     2761 ttccttaaag agaagtaaaa tgcctgccgt tatatttcgg actgccgata agacacgacg
     2821 atgatcatca gaggaagatt ggtagtcctc tattccaatt tgaattgact gcacggcatt
     2881 tttaaggatg gtgttcattt gaaatggaaa caacctatat taataaaagc tgctgtactc
     2941 cattctattc ggatggtgaa gtgatattaa aacttataaa taataaatca tcttttttgt
     3001 gtaactttca ataacatatc ttttaggcag gcacataggt agaaatctta atatttccgc
     3061 atttctgttg cgtcatacca tgcctgcatc ctgagcaaca tatccatgtt gagttgaaaa
     3121 gccttttcta ctcgtagggc catttccgcc gaaagcgcgg ctttttcatt cactaaatcc
     3181 gacagagtgg cgcggcgcac acccaaaact tcggctgctt tcgtaacact tacgccgagt
     3241 tcgtctataa cttccgactt aatgaacgca cccggatggg acggcttcat cttgattttt
     3301 aattctttca ttttcagaac aactacttca ttgttattcg tcgaccagcc gtttcaatcc
     3361 attattatct ttaatatgca accattcccg ctcctcacaa ccaagaccag aagaattaca
     3421 tagtaaaacc gtgccagtag aaactagatc gggaatgcct cggatcaacc atatagactg
     3481 ccaaatagtt tcagagtcgg aaataaggtc catgagttct tccaaccctc gtccaactgg
     3541 cccaccacca tgaatgttca cgtatacaag gaggtggcca ttgaatccat caattctccg
     3601 agctttctca gttttcttgc agatcaccgg agcaatccat tctggattca ttggggttcc
     3661 agacagtgtt tgcataggaa aggaggggcg tctcccattt ttatactgct ctctaatctt
     3721 ataatctggc ggatacgctg tggttaattc aaattgaaaa gccgaatttt cctgagaaat
     3781 aagttcaaag tctagaagtt tttcgtgcac ttttgccatt cgtatctgaa ctggcgatat
     3841 atgctcctga aacaccagtg caaatgtaga agcgacgaga gcttcttgga gtttttgtac
     3901 aaagtctttt ccacgggaaa gaagatcttc atctgacatg gaggatgcta tttgattcgc
     3961 aaggcaccac aagtcccatg gagtttgtag ctggcagaat ttattcaacg accccataaa
     4021 tttcaagaag ccctgttaca taattgctta aaaaaatcgg gtatgccttg cctcttacct
     4081 gatgatagct tttatcagaa ataaacaaaa tcccttcttt ttggccttcc tatgtccccg
     4141 acaatggcga gggagaatat tccaataatt gcgacactca ccttcctttt ccctgacgcc
     4201 tgctggctgg ccccaggggc gaacgctcta accaatcggc agcccttgtc cgagcccttc
     4261 gccaaaactc aggacaggct ccgcgagttg ggctgccctc cgaaggctcg cgtccgcccc
     4321 atccgatgtg gccaggcagg gcgtcaatgg ttttggctac ttttgccgga acaaaagtgg
     4381 ctcggctgcc gggccgaaac ccggcaacac tcgtataggc caatcaatcc agttgatgaa
     4441 attttgcgct cgaacatgtc ggaaaatatc atgagctttt cgttgccaac catgtggtga
     4501 gcttctacac tcactgttat gtccaatccc actgatcagc tctcttcacc agatccggcg
     4561 gcgcccgttt ttcggcctgg agatttcaag ggccgtgagg gattagtccg caatatgttg
     4621 cggcggttgg aacgagggga gtctttgagc cttgttgggg ggccaaagtt aggcaaaacc
     4681 tcgatgctgc ttcaccttgc ctggcaattg aatcatgtgg gcccttcccc caagtccacc
     4741 ggtccttctg cgttgtacgt ggatgttgca gatgaggctg attggaaacg atttcattcc
     4801 cgcccgccaa atcctgaaac cgttcttctc cttgataact gtgaccggct ggttgaaggg
     4861 aagactcgtt cgctttctga catcgatctt ttgccgggag ggtccacggt ctttgccgga
     4921 ggacgagcat ggcgggaggt ggtgagagaa ggcaatttgc cacacacact aaagttgatc
     4981 cccttgtcgg tgtttttgga aaaggaagcc cagcagctat tcagtcggga tctttccgca
     5041 gaacaacagg ccactgtcct cacctatgcc gggacgcatc cctatatctt caagctgttc
     5101 caggctgcat tcctgcataa agaaatccat attccgacgg aacagattgt cagcgaggtg
     5161 aaaaaatccc tctccttctt tttccaagat tgtatcaatc aactgcggga acctctcgaa
     5221 catcaagtgc tgacctttgt ggttgaagca aacaaacccg tgaacccacg agaggtggcg
     5281 cgggccgttg gcctgccgac gatcaagccg gttgccgata cgttgtgtgc gttgggattg
     5341 gttgccaggt ggattcggga tgaagaagcc accctttcag caagcagccg gatgtttaac
     5401 gaatggtata gggaaacggt gatttcctaa gatatgatag cccggtggcc tcatgatgag
     5461 gcaaacaggg tgggcgttct ctatttagtc tgtacatgaa aaagaggaac ttttcgtggg
     5521 agcgtttaaa atattttctg cggaattcaa ggcgagttgc gggacggttg agcaatttct
     5581 gaaaccgacg cttccggaag tggccatcgt cggccgttct aatgtcggga aatcctcggc
     5641 cattaattgt ttggtgaatc agaaagggtt ggccaaggtc ggtaagaccc ctggaaaaac
     5701 ccaaaccatt aatttttttg agattgccac gaacggccca cgttttatgc tggtcgatct
     5761 tcccggatac ggatttgcca aagtgcctga gcggattcga gagctctggg gtcccctgat
     5821 tgaagaatat ttacggacac gcaaaaattt acagggtgtg gtggtgctgg tggattcccg
     5881 acgggtacag gaatcggatc gggcgatggt cgagtggctc atgcggctga agattcccgt
     5941 gatggtggtg gccaccaagg cggataaggt cacaaggggt catcgccagg ctgcgctgag
     6001 ggaattgcgt gagggtttgg gtatggagga ggacccgctt ttcctgtcgg cctataccgg
     6061 ggaaggcaag cagttgctgc ttgaccagtt gcgggaggtg cttactctgg acgctcccct
     6121 tccctaaaag ggggtgttta aaagaccgcc agcgttgttc ccaccgcttg gtcgccttcg
     6181 gacgcacaaa cttgacgctc ttcggttcgt gcgcgcccac caattcctcc gtacgctccg
     6241 ctcgcccaaa cggctgcggc cttgccgcgg gaaatggcat gcttgtatgc cgggccaggc
     6301 gagtgagaac acgcgccttc cttcagccag actcaggaca agctttgtaa actcccttat
     6361 ctttccccat actgagttct gtcactcctc ataatgatga aaggccaaat acgagaaata
     6421 ttcaacagcc tccgaatgac attcttttcg aatccatccc ataagttttc attcctggtt
     6481 ttatgtcgaa gttgaagaac ttccctaaga tctcctattc tgacgggtcc cccaattttt
     6541 catatgttgt ctattcatgc acggccggcg aatggaatag ccacctctcc ctgtgtattt
     6601 attcatttct caatacgaag gaaagctatg ccactgtttg gtgatacgaa tacacccatg
     6661 accttcaacc ctccctccag acttgatcgg atcgggccat cgcagattcg cgaaaacatg
     6721 cggattgcca tggagcatga agcgattaac ctggctcaag gccgtcccga ctttcccacc
     6781 gcacctgtgg tgaagcaagc cgcaatcgat gccattgcca atgatcacaa tcaatattcg
     6841 gtgacctggg gattggcaga gttgcgggaa gccattgcca ggcatgtgca cgaacgccac
     6901 ggtctcacct ttgaccccga aacagagatc accattacct gtggtgtgac ggaagcgatc
     6961 gtggcggcta tgctggcatt agtcgagagt ggtgatgaag tcatcatcat cgaacccgca
     7021 catgaaaact atgttccggc tgtgtatttt gccagcggta cgccacgatt tgtcacg
//
LOCUS       DATIAL010000591         8409 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1394847, whole genome shotgun sequence.
ACCESSION   DATIAL010000591 DATIAL010000000
VERSION     DATIAL010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394847"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            696..1190
                     /locus_tag="VLA60_17825"
     CDS             696..1190
                     /locus_tag="VLA60_17825"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HSF11274.1"
                     /translation="MAEEDEKGEAPVKAGAGKKMLLIIIAGVLLLGGGGGAAWYFMSG
                     QEEKPAEHEEHAKAETGHEEPGPVMELEPFLLNLADREELRFLKVSIKLELDRPEEKT
                     DYQHKIPAIRDALLVLLSSKESQLLRTVNGKRRVREEIMTRVNGVMSKGRVANVYFTD
                     FIIQ"
     gene            1269..2273
                     /gene="fliM"
                     /locus_tag="VLA60_17830"
     CDS             1269..2273
                     /gene="fliM"
                     /locus_tag="VLA60_17830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HSF11275.1"
                     /translation="MPRPNMEKILSQDEVDALLRGVSDGGVETEPEDPVGAELQDGPV
                     PYDLGNQEWVVRGRMPTLDVIHQQFSRGFRLSLSDVLRKTAEVTVTNQTVMKFGEFTK
                     RLPIPAYLQIISMEPLRGLAMVATDAATVYLLVDHFFGGTGQTHVKPEGQDFTIIEQR
                     VMKKVMLMGLGNLQKAWDPVQKLLINAVRVEMNPQLAAIVLPADIVIVVTLGIELGNS
                     VGDLHLCLPYAMLEPIRERLQVSFQGDSFETDHGWLKRFSARLKEAYVTLSVCLGETE
                     ISVEELMQFSPGDVIGLNQGTTQLLTATVEGVPKFLGSPGTTKGMQSFQIKDIILTRE
                     "
     gene            2335..2760
                     /gene="fliN"
                     /locus_tag="VLA60_17835"
     CDS             2335..2760
                     /gene="fliN"
                     /locus_tag="VLA60_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HSF11276.1"
                     /translation="MSEDDNIDMDTFDAEKEMMEALAQEAAAKEQNAASKKQEVAASK
                     SSQTPQLNTSHDQNLNRILDIPLVLSAQLGNTRMLIKDLLQLGPGSIVELDKLAGEPL
                     EVLVNERLVARGEVVMVNEKFGIRLTDVISPTERVNKLR"
     gene            2846..3280
                     /locus_tag="VLA60_17840"
     CDS             2846..3280
                     /locus_tag="VLA60_17840"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HSF11277.1"
                     /translation="MDLTHEALKMAGTLALVVLVLLGGLAWVRRTFGELPGKSGDPVM
                     RILGGLRVGTGKHIMLVEVAGEVLVLGTTAREMTLLTTVADADRIDRLRSIGNPIVGP
                     LGAWLGQWTRQAFEKSERGSVTSEPSIRMSKSVTRVRGEERS"
     gene            3277..4056
                     /gene="fliP"
                     /locus_tag="VLA60_17845"
     CDS             3277..4056
                     /gene="fliP"
                     /locus_tag="VLA60_17845"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HSF11278.1"
                     /translation="MNNPFCGIRGGRDCWGLLGMSLVMGLPTSAFAQVTKDPAISLQV
                     SGLDGTEPWTFGLRILFLLTALTLAPALLMLVTAFTRVVIVLGLLRQALGTMHAPPNQ
                     VMIGLAMFLTLFIMMPVWEQIRVQALNPLLEQQVTQEVALDRAKIPLRMFMLKQVREK
                     DLTLFVEMAKVPHPQTPDDVPFHVLVPAFVTSELRTAFQIGFLIYVPFLVIDMIVASI
                     LMSMGMMMLPPIMISLPFKLVLFVLADGWFLVVGALMNSFQ"
     gene            4213..4482
                     /gene="fliQ"
                     /locus_tag="VLA60_17850"
     CDS             4213..4482
                     /gene="fliQ"
                     /locus_tag="VLA60_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HSF11279.1"
                     /translation="MTTESVLSIGQEAIQTILLVAGPMLGLSLLVGLCVSAFQAMTQI
                     NEMTLTFLPKVATMFVVLLVTFPWMVEILVGFMTGVWARIPEFAQ"
     gene            4546..5316
                     /gene="fliR"
                     /locus_tag="VLA60_17855"
     CDS             4546..5316
                     /gene="fliR"
                     /locus_tag="VLA60_17855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HSF11280.1"
                     /translation="MAIQQVWQFVVVLVRTAVILSALPLLGGQSVPSRIKIGMAVIIA
                     ILLMPIVTFTLPPNWLEPGNLVIVLGAELLVGLVLGLALRLLMTAVELAGSVMGFQVG
                     FAMAGVLDPVTQVETPVFGQLLTILATLLYFQVDGHHLVLLALGSSFLLIPPFGAHLS
                     APLLSDVTEVIQRTYDTGMKLAFPVMGATFLVHFIMGILGRLVPQMNVILTSFPITIA
                     VGLLVLGLGLPFIALVFQDSIVGMETVLWDLLQELGHG"
     gene            5309..6385
                     /gene="flhB"
                     /locus_tag="VLA60_17860"
     CDS             5309..6385
                     /gene="flhB"
                     /locus_tag="VLA60_17860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HSF11281.1"
                     /translation="MAENKDGADKSEQPSPKRLAEARRKGQVPMTRELPSLFVLLGGV
                     GLLSLWAPHAFVQFFDHYRQWLAQAGTLQLDAQSTHVLLLNIVYQAFVPLIPFGLLVG
                     VFAFLAIILQTGPLWIEEALQPKPSKLNPSNGLKRIFSWKGVVDLLKSLLKLAGVSGI
                     AYLILSHNLLAIVQLPLLGLTEAVGGVWGLLENIMWSVGGSLLLLAIIDFVYQRWQHT
                     EDHKMTKQEVKDESKDVEGDPQIRSRRLSLQRERARQRMLQAVPKADVVITNPTHVAV
                     ALRYETGKMDAPVVVAKGAGFMADKIKQIARHAGVPIIENRSLARGLYKAVKIGQEVP
                     SALYQAAAEVLAYVYRLKKIHEGT"
     gene            6547..>8409
                     /gene="flhA"
                     /locus_tag="VLA60_17865"
     CDS             6547..>8409
                     /gene="flhA"
                     /locus_tag="VLA60_17865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HSF11282.1"
                     /translation="MAFDGQKLRDELTPAHYGDVLLPIGVVGMLLLMLLPLPSFLLDL
                     FLSFSITLAVLILLVTMHAGRPAEFSVFPSVVLLTTLFRLALNIASTRAILMHGNEGP
                     AAAGQVIAVFGEFVVGGNYAVGIVVFSILVIINFVVITKGATRIAEVAARFTLDAMPG
                     RQMSIDADLNAGIIDEGEARKRREAISQEADFYGSMDGASKFVRGDAIAALLIIFINV
                     IGGLGIGVLEHGMSIIDAAKRFTLLTVGDAIVAQIPALIMSTAAGIIVTRVSTKTSLG
                     RDLTDQIFFNPHLVAAVAGMLFFLGFLPGLPHVAFLLLGSMTAGLAYVLWNQKQEAAR
                     LKNLPKKKDPAISSGTESVDSVTPPDLMELHVGYGLVPFVDQAQGGELLERIRAIRRQ
                     TALDMGFVVPAVHIRDNLQLKPHEYNILLKGIQVAKGELVPRHLLAINPGHAKKGLQG
                     KPGKDPCFGLPAVWIAPTEKERAQMDGYTVVDGPAIIATHMTEFIKTNGHELLGRQEV
                     QNLLDNLAKTYPKVVDDLIPTQISLGAVVRILKNLLRERVSIRDLRTILETVADYTTQ
                     SKDPDTLTEYARQNLSRTISSQYANPDGLLQVISLDPMLDRRLMEAMRPSGNGEQ"
BASE COUNT         1890 a         1926 c         2466 g         2127 t
ORIGIN      
        1 gatctaactg atcaagcccc caggcaggaa cacctgcctg ggggcttttt tttgccttag
       61 tcggatgttg aacctaaaaa tggccgttgg ccatcctgga acccttcgac tgccgcggaa
      121 gccgcggcgc tcagggcgaa cggactctca cccaacgggt gaccacttcg acaccccgtt
      181 cctgctgagg ccctcgaagc atgaacgggc tagcagccct gtaacggccc tttttaggtt
      241 gaacaatcat gcctcatata accgtgaggg aggtaaggag taagtcgaaa agaatacttg
      301 ccttaatttg ctgaatacat ctttacttct cactcctttc gtttttcctt tcacaccccg
      361 cactcctgac tcttcacgcc tcacgttttt cacgtcctac tccgtacgca tgacgcctga
      421 cccctcacgc gttactcctt acgcctcacg ttctcataga cgaacctgtc aaaggttccc
      481 tcgaattgcc aggaaaatga cgaaaaggca aatctcgtgt tgttggaggg cataggaaat
      541 gtaaggcacg actgcggaag aaacatatgc atattcagtg cgttacaggc ttagccgcag
      601 cctgtctacg tagaaaattg gggtggcaca aaggttgctg acttgggaag gggaacgtga
      661 aggtccaaaa acttcttagc gtgtgaggtt gatatatggc tgaagaggat gaaaaagggg
      721 aggcccccgt taaagccggg gccggaaaaa aaatgttgtt gattattatc gcgggcgttc
      781 ttcttctcgg tggaggggga ggcgcagcct ggtatttcat gagtggccaa gaggagaagc
      841 cggccgagca cgaagagcat gccaaagccg aaacaggtca tgaagagccc gggccggtga
      901 tggagttaga gccctttctt ctgaatttag cggatcggga ggagctgcga tttttaaaag
      961 taagcatcaa actcgagtta gaccggcctg aagaaaaaac ggattatcaa cacaaaatcc
     1021 cggccattcg cgacgcgttg ctggtgttac tgagcagtaa agaatcgcaa ttactcagaa
     1081 cggtgaacgg aaagcgccgt gtgcgagaag aaatcatgac gcgggtgaac ggcgtcatga
     1141 gtaaagggag agtcgctaat gtgtatttta ctgattttat tattcagtag ggcctgagga
     1201 gaaagacgta agaggtcttt agtcaggaat cagaaagagg agagaagaga cagcaggact
     1261 gtttctttgt gccacgtccc aacatggaaa aaattcttag tcaggatgaa gttgatgccc
     1321 ttcttcgggg cgtgtccgac ggcggcgtgg agacggaacc tgaagacccg gtaggtgccg
     1381 aactacagga cggaccggtt ccgtatgatt tggggaatca ggaatgggtc gttcgcgggc
     1441 gaatgccgac cctggatgtg atccaccagc aattttcgcg tgggttccgc ctctctttga
     1501 gcgacgtgct tcgtaaaacc gccgaggtga cggtcacgaa ccaaacggtg atgaagttcg
     1561 gggagttcac caaacgtctt cccattcccg cctacctcca gattatctcc atggagcctc
     1621 ttcgcggctt agcgatggtc gccacggatg cggcgacagt gtatttgctg gttgatcatt
     1681 ttttcggggg aaccggacag acccatgtga agccggaagg gcaggatttt accatcattg
     1741 agcagcgcgt gatgaagaag gtcatgctga tggggttggg gaatcttcaa aaagcctggg
     1801 atcctgtaca aaaactcctc atcaatgcgg ttcgggtaga aatgaatcct cagttggccg
     1861 ccattgtgtt gccggcggat atcgtcattg tggtcacctt gggcatcgaa ttaggcaatt
     1921 cggtggggga tctccacctc tgtcttccct atgcgatgtt ggaaccgatt cgagaacgtt
     1981 tgcaggtgag tttccaggga gactcgtttg aaacggatca tggctggttg aaacgatttt
     2041 cagccaggct gaaggaggcc tatgtcaccc tgtccgtgtg tttgggagaa actgaaatct
     2101 cggtggagga attgatgcaa ttttcaccgg gagatgtgat cgggttgaat caagggacca
     2161 cccagctgct cacagccacg gtggaagggg tgccaaagtt tcttggttct ccgggaacga
     2221 ctaagggcat gcaatcattt cagatcaagg acatcattct gacgagggag taagggccgg
     2281 tgaacgggat aaggtgtcgg ccagtcactg actgtggaaa cccggagatg aacgatgagt
     2341 gaagacgaca acatcgacat ggacacgttt gatgctgaga aggaaatgat ggaggcgtta
     2401 gcgcaggaag ccgctgcgaa agaacagaat gctgcgtcca aaaaacagga ggtagcggcg
     2461 agtaaaagca gtcaaacgcc acaattgaat acgtctcacg atcagaactt aaatcgtatc
     2521 ctagatattc cgttggtgct ctctgcgcaa ttgggcaata cccggatgct gattaaggac
     2581 ttattgcagt tagggccggg ttccattgtt gaattggata aactcgccgg tgaaccgttg
     2641 gaagtgttgg tgaatgaacg gttagtcgcg cgtggtgagg tggtgatggt gaatgagaaa
     2701 ttcggcatcc gtctcaccga tgtgattagc ccgaccgagc gtgtgaataa acttcgatag
     2761 ggacgtaagg acggaaggcg tgaggggtca ggcgtaatgg ggagatataa tttacgtctc
     2821 acgttttacg ccgaacgaaa aaaatatgga tcttacccat gaagcgctca aaatggcggg
     2881 caccctggcc ctggtggtcc tggtccttct tggaggatta gcctgggttc ggcgcacatt
     2941 cggggaattg ccgggaaaga gcggcgatcc ggtgatgcgc attttaggcg gactccgggt
     3001 cgggacggga aagcatatca tgctcgtgga ggtggccgga gaagtgttgg tgttggggac
     3061 gacggcccga gagatgacgt tgctgacgac cgttgcggat gccgaccgga ttgatcggct
     3121 ccgctccatt gggaatccga tcgtcgggcc gctaggtgcc tggttggggc aatggaccag
     3181 acaggcattt gagaagtccg agcgaggatc ggtgacctcc gaaccgtcca tccgaatgtc
     3241 caaaagcgtc acacgggtga ggggagaaga gaggtcgtga acaacccctt ttgcgggatt
     3301 cgtggaggac gtgactgttg gggactgtta ggcatgagtc tggtcatggg actgcccacc
     3361 tcggcttttg cccaggtcac caaggatccg gcgattagtt tgcaggtatc cggtttggac
     3421 gggaccgaac catggacttt cgggcttcgt atcctctttc tcctgacggc cttaacgctt
     3481 gcgccggccc tgttgatgtt ggtgacagca tttacacgtg tcgtgattgt tctgggtctt
     3541 cttcggcagg ccttggggac catgcatgca ccaccgaatc aagtgatgat tggattggcc
     3601 atgtttttga cgctgtttat tatgatgccg gtgtgggaac agattcgggt ccaggccctt
     3661 aatccgctat tggaacagca ggtgacacag gaagtggcgc ttgatcgggc caagattccg
     3721 ttaagaatgt tcatgctgaa acaagtccgg gaaaaggatt taaccttgtt cgtggagatg
     3781 gcgaaagttc ctcaccctca gacgcccgat gatgtcccgt tccatgttct ggtgccggcg
     3841 tttgtcacga gtgaattgcg cacggcgttt caaattgggt ttctcattta tgtgcctttc
     3901 cttgtgatcg atatgattgt cgccagtatt ctcatgtcca tgggtatgat gatgctgcca
     3961 ccgatcatga tttcgttgcc gtttaaattg gtgttgtttg tgctggctga tggatggttt
     4021 ttggtcgtgg gagcgctgat gaatagtttt caatgaagaa cgtgaagcgt aaggcgtaag
     4081 tcgtgtatcg aatgctgagg tgcaaagatt ggttctttct tctgtctcct tacgcttcac
     4141 gccttacgcc tcatagtttt tcttcacgac taatcgagtg tttttcacgg caagactgaa
     4201 aggggtaagg agatgacaac cgaaagtgtc ctgagcatcg gtcaggaagc gattcaaaca
     4261 atcctgcttg tggcgggtcc aatgttgggt ctcagcttac tggtcggact ctgtgtgagt
     4321 gccttccaag ccatgacaca aattaacgaa atgaccctca cctttctgcc aaaggtggcc
     4381 accatgtttg tggtgctctt ggtcacattt ccttggatgg tggaaattct tgtggggttt
     4441 atgacggggg tatgggccag gatacccgag tttgcccaat aatagaggga gtcgacctgc
     4501 ggcatgtatc agaaagatgt ccggtaaccg gtgttgcaat gggatatggc gattcagcaa
     4561 gtgtggcaat tcgtagtcgt gctcgtgcga acggccgtta tcttgtcggc gttaccgtta
     4621 ctgggtgggc aatcggttcc gagtcgcatc aaaatcggca tggcggtgat catcgccatc
     4681 ctgttgatgc ccatcgtgac gtttaccctt cctcccaatt ggttggaacc tggaaatctg
     4741 gtgatcgtct tgggtgccga attgcttgtc ggcctggtct taggtttggc cctgcgatta
     4801 ctcatgaccg cggtggaact ggcgggaagt gtgatgggct ttcaagtggg gtttgccatg
     4861 gccggagtgt tggaccctgt tacgcaagtg gagactccgg tgttcgggca attactgact
     4921 attctggcaa cccttctgta ctttcaagtc gatgggcatc atttggtgtt gttggccttg
     4981 ggcagtagtt ttctgttgat cccccccttt ggcgctcatt tgagcgcccc cctactgagc
     5041 gatgtgactg aagtgattca acgcacctat gacacgggaa tgaaactggc cttccccgtg
     5101 atgggggcca cattcttggt tcatttcatc atggggattc ttggccgcct ggttccccag
     5161 atgaacgtga tcctcaccag ttttcccatt acgattgcgg tcggtttatt ggtgttaggc
     5221 ctgggattgc cctttatcgc cttggttttt caagactcca ttgtcgggat ggaaacggtc
     5281 ctgtgggact tattacagga attagggcat ggctgaaaat aaagacgggg cggataaatc
     5341 agagcaaccg agtccgaaac gtttagcgga ggctcgtcgc aaaggccaag tgccgatgac
     5401 cagggaattg ccctcgttgt ttgttttgtt gggtggggtt gggctgctgt ctctgtgggc
     5461 gcctcatgcg tttgtccaat tttttgatca ttaccgacag tggttggcgc aagccggaac
     5521 ccttcaatta gatgctcagt ccacacatgt cttgttgtta aatattgtct atcaggcctt
     5581 tgttcccctg attccgttcg ggctcctggt cggggtattc gcctttctcg ccattattct
     5641 gcaaacggga ccactttgga ttgaggaggc cttgcaaccc aagccgtcca aattgaatcc
     5701 ctccaatggg ttgaagcgca ttttttcctg gaaaggggtc gtcgatcttt taaagtcctt
     5761 gctcaaatta gcgggggtga gtggcattgc ctatctgatt ctgtctcata acctgctcgc
     5821 gattgttcaa cttcccctcc ttgggttaac ggaggcggtt gggggtgtgt ggggtctcct
     5881 ggagaacata atgtggtcgg tcggagggtc cctgctcctg ttggcgatta tcgatttcgt
     5941 ctatcagcgg tggcagcata ccgaagatca caagatgacc aaacaggaag taaaagacga
     6001 gtcaaaggat gtggaaggtg atccgcagat tcgatcccgc cggctcagtt tacaacggga
     6061 acgtgcccga caacgaatgt tgcaggcggt gcctaaggcc gatgtggtga ttaccaaccc
     6121 gactcacgtg gctgtggcgc tcaggtatga aacaggaaaa atggacgctc cggtggtggt
     6181 ggcgaagggc gccggattta tggcggacaa gataaagcaa atcgcgcgtc atgcgggagt
     6241 gccgattatt gaaaaccgca gtttggctcg aggactgtat aaggccgtga aaatcggtca
     6301 agaagtgccc agtgcgttgt atcaggcggc cgctgaggtg ttggcctatg tatatcgcct
     6361 aaagaaaatt catgagggaa cgtgaagggg taaggggagg aaagtaagga gacagaagaa
     6421 agaaattttt gcctctccgt attctctacg cctcacccct cacgcctcac gcctcacgaa
     6481 gttgaggaga gtcgattgga aatacaatgc aaccatatgt aacagcggta accgattaag
     6541 gcacgcatgg catttgatgg acaaaaacta cgagacgaac tgacccccgc gcattatggg
     6601 gatgtcctct tgccgatcgg ggtggtgggg atgctcctcc tcatgctctt accccttccc
     6661 tcctttctgt tggacctttt tttatccttc agtattacgc tggctgtctt gattttactt
     6721 gtgaccatgc atgccgggcg tcctgcagaa ttttccgtct tcccctcggt ggtgttactc
     6781 accacgttgt tccggcttgc gctgaacatt gcttcgacaa gggccattct catgcatggg
     6841 aatgaaggcc ccgctgcagc cgggcaggtc attgccgtgt ttggggagtt tgtggtcggg
     6901 gggaattatg cggtgggcat tgtggttttc tccattctgg tcattatcaa ttttgttgtg
     6961 atcaccaagg gcgccacccg tattgcggaa gtcgccgcgc gatttaccct ggatgcgatg
     7021 ccaggtcggc aaatgagtat cgatgcggat ctgaatgcgg ggatcattga cgaaggggaa
     7081 gcgcggaaac gccgggaggc catttcacag gaagccgatt tttacggatc gatggacggt
     7141 gccagtaagt ttgttcgtgg cgatgcgatt gcggcgttgc tcattatctt cattaacgtc
     7201 attggtggat tgggcatcgg cgtgctggag catgggatgt ccatcattga cgctgctaaa
     7261 cgcttcaccc tgctgacggt cggggacgcc attgtggctc aaatccctgc cctgattatg
     7321 tcgacggctg ccggtatcat cgtgacgagg gtttcgacca aaaccagcct tgggcgtgat
     7381 ttaaccgatc aaattttttt caatccccat cttgtggcgg ccgtggccgg catgttgttc
     7441 tttttaggat ttcttcccgg gctgccgcat gtggcgtttt tgttgttggg atcgatgacc
     7501 gccgggctcg cctacgtatt gtggaatcag aagcaagaag ccgctcgcct gaaaaatctc
     7561 cccaagaaaa aagatccggc gatctcctcc ggcacggaat cggtggattc cgtgacccct
     7621 ccggacctta tggagttgca tgtcggttat ggacttgtgc cgtttgttga tcaagctcag
     7681 ggaggggagt tgttagaacg gattcgggct atccggcgcc aaacggccct tgatatggga
     7741 tttgtcgtgc ccgccgtgca tatccgggat aatcttcaac tcaaaccgca tgagtacaat
     7801 attctgttga aggggattca ggtggccaag ggagaattgg tgcctcggca tttattggcc
     7861 attaatcccg gtcatgccaa aaagggtctg caaggaaagc cgggaaaaga tccgtgtttc
     7921 ggattgccgg ccgtctggat tgctccgaca gaaaaagagc gtgctcaaat ggatggctat
     7981 acggtcgtcg atggcccggc cattattgcc acgcatatga ccgaatttat taaaacgaat
     8041 gggcatgaat tgctgggtcg gcaagaggtc cagaatttgc tggataacct cgctaagacg
     8101 tatcccaagg tggtggatga cttgattccg actcaaatat cgttaggagc ggtggtgaga
     8161 atcctcaaga atctcttgcg ggaacgagtg tcgatccgcg atttgcggac cattttggag
     8221 actgttgcgg attacaccac tcagagtaag gatcctgaca ccctgaccga atatgctcgg
     8281 caaaatttat ccagaacgat ttcgtcccaa tacgccaatc ccgacggatt actgcaagtg
     8341 attagtctgg atccgatgtt ggatcgccgg ttgatggagg cgatgcggcc gagtggaaat
     8401 ggagagcaa
//
LOCUS       DATIAL010000592         3463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_429786, whole genome shotgun sequence.
ACCESSION   DATIAL010000592 DATIAL010000000
VERSION     DATIAL010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429786"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="VLA60_17870"
     CDS             complement(<1..902)
                     /locus_tag="VLA60_17870"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HSF11283.1"
                     /translation="MVDVFLPILASLALLIIAGTLLPLFRVGEWWIRIFDFPRVQILT
                     IGAAVALGLGYLGFSSDNTLWQWVLIPLIGCLVYQGHRIYFYTMFAPIQALDSKNPRK
                     EALLSIVVANVYMNNRNASAFLDLVYAFSPDVVLVTEPDQWWERQLRGLESSYPYTIK
                     RPQENTYGMLLYSRIKLRNPAVRFLIEDSIPSIFTELELPMGVVIDFYGLHPRPPHVG
                     QDSEERDAELLTVGREVEHAHRPVVVAGDMNDVAWSYTTSLFQRISRLVDPRLGRGFY
                     NTFHARYPILRFPVDHVFHSSSFR"
     gene            1680..2036
                     /locus_tag="VLA60_17875"
     CDS             1680..2036
                     /locus_tag="VLA60_17875"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HSF11284.1"
                     /translation="MSNTFTNQLRDKALLVVFSAACAFAFSACASDQHGRTVGTTVDD
                     AVITSSVKSSLFADDLVDAFEIEVDTYRSTVMLSGFVETQNQIERAVEIAKKTEGVQK
                     VVNKLAIKPKALSQKN"
     gene            complement(2223..2837)
                     /locus_tag="VLA60_17880"
     CDS             complement(2223..2837)
                     /locus_tag="VLA60_17880"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HSF11285.1"
                     /translation="MEIDRPSGLSKPGDTHSPETPVSPALWAIGHSTRPIEEFLGLLL
                     TRGIRVLVDVRTIPFSRRNPQFHQESLAHCLREAGLQYQHMPELGGRRKTRPDSVNVG
                     WRNAGFRGYADYMQTQEFWDGLEELVNIGQQSPSAVMCAEAVPWRCHRTLIADALVIR
                     GWTVHHIISASSLKTHTLTSFAKPDAGRLTYPLDSPSDLNLRLF"
     gene            3098..3433
                     /locus_tag="VLA60_17885"
     CDS             3098..3433
                     /locus_tag="VLA60_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11286.1"
                     /translation="MRIPFSSCFSVFSSVHAHQLFLSWFVLGGILSGSPLFAAGSFQE
                     DCLGKSPAGIALQSHLVAAKSGKVDSMFCAGAIHLYVQHDLNKALPWLEKATNAGDKR
                     APLVLGILY"
BASE COUNT          933 a          878 c          833 g          819 t
ORIGIN      
        1 aatcgaaatg aggaagaatg aaacacgtgg tccacgggaa accgcaaaat cggatacctg
       61 gcgtggaagg tattgtagaa tcctcggccc agtcttggat ccactaatct gctaatcctc
      121 tgaaacaaac ttgtggtgta cgaccaggcc acatcattca tatctcctgc gaccaccaca
      181 ggtctgtggg catgttccac ctcccgtccc acagtcaata attccgcatc ccgctcctcc
      241 gaatcctgtc ccacatgagg agggcgagga tgcaacccat agaagtcgat tactaccccc
      301 atcggaagtt cgagttctgt aaaaatagag gggatcgaat cttcgatcaa aaagcggact
      361 gcagggttgc ggagttttat tcgtgaatac aggagcatgc cataggtgtt ttcctgcggc
      421 cttttgatag tgtaaggata ggaggattcc aggcctctta actgcctttc ccaccattga
      481 tccggttcgg tgacaagcac cacatcagga gagaatgcat acacaaggtc taaaaaggct
      541 gaggcattcc gattgttcat atacacattg gcgacaacaa tactcagcaa ggcctccttt
      601 cgcgggtttt tgctgtccaa tgcctgtatg ggagcaaaca ttgtgtagaa ataaatccgg
      661 tgtccctggt agacaagaca tccgataagg ggaattagca cccactgcca taaagtgtta
      721 tctgaagaga atcccaggta tcccaacccc aaggcgacag ccgcgccaat ggtaaggatc
      781 tgaactcgag gaaaatcgaa aatacgaatc caccactcgc caacccgaaa caggggtagg
      841 agggtgcctg ctatgatcag caaagctagg gaggccagaa taggaagaaa tacgtctacc
      901 atgctacacg cataacccca tcaccatact gtttaagggc gaaagttata accttcctat
      961 tcaacacacg attgaaggta gtaaaggcat tttaagaaaa cggaatggtc agcaggcatg
     1021 agtgcccgca atcgtagaaa agctcacccg ccgacttggc aggacaattg aaattaacca
     1081 tcgcttctat gtgtctacct gcaagagagt cccgaatcca atagataaat ggcgaaaaaa
     1141 tctgagaaaa aatccaacct gagttcgatg agtgtcaatt ggggaatacg ctgcctccac
     1201 cgcgaaacac aaccggagga ttgactcaca cctggcaacc gccaaagtcg atagctctct
     1261 gttagggttc aaggttgatt ttgtcatgaa aagcactctc aaacccttaa ttggccaaca
     1321 tatcctcagt gaggttttga gcctatgaaa ggaaggcgag aattcctcga ctttctggcc
     1381 aaacccttcc gaagatcccg ttcggtgaaa attccctttt gtttggcttt tccaatcttg
     1441 ctgagtatcg agaaacctcg atttcagtct ttcttgcatg gcaactattg gcgcttcctg
     1501 gatcccagta gaagagtctc ttctttggga aacgggaaag agcaaggtat tttgagtcag
     1561 gtatcaaggg tttttgtcac ttctgcctcg ctcccctttg aatcatactg acttagtcta
     1621 ttgttgaatg gcatcacggc tgtgtccaaa aactttcatt tcataaaggg agaaatgtta
     1681 tgtccaacac attcacaaac caactgagag acaaagcctt gttagtcgta tttagtgcgg
     1741 catgcgcatt tgcattttca gcatgtgcca gtgatcagca cgggcggact gtggggacca
     1801 cggtggacga tgccgtgata acctcatcag tcaagtcatc attatttgcc gatgacctcg
     1861 tggatgcgtt tgaaattgag gttgatacgt acagaagcac tgtcatgtta agcggatttg
     1921 ttgaaacaca aaatcaaatc gaaagagccg tggaaattgc caaaaagacg gagggagtgc
     1981 agaaggtcgt caataaatta gcgataaagc cgaaagcgct ttcccagaag aattaaccag
     2041 ggatatcgaa atgtcagaac ttcccaccta gaacaactgt tccccctgga aaaattccag
     2101 ggggaacatt accaaaaacc ctggcttcga gcctgtcggc cgccaatctt attggaatca
     2161 tgcaatacac atcccaggag tcttccaacc aacaaattca aacgcacttt tccatgcttt
     2221 tgttagaaga gccggagatt gagatctgaa ggactatcta atggataggt gaggcgacct
     2281 gcgtcaggct tggcaaagga cgtgagtgtg tgcgttttga gagaacttgc cgagatgatg
     2341 tggtggacgg tccaaccacg gatgactaat gcatcggcta tgagcgtccg gtggcagcgc
     2401 cagggtaccg cttccgcgca catcacggct gatggtgact gctgtccgat attcaccagt
     2461 tcctccagcc catcccaaaa ctcttgagtt tgcatgtaat ccgcgtaacc tcggaaaccc
     2521 gcattccgcc atcccacatt caccgaatcc ggccgggttt tcctccgacc tcccaactcc
     2581 ggcatgtgct gatactgcag cccggcttca cgaagacagt gcgcaagtga ctcttgatgg
     2641 aactgaggat tccgccgaga aaagggaata gtccggacat cgaccagaac tcgaatgccg
     2701 cgcgtcagaa gaaggccgag gaattcctcg ataggccgag tcgaatggcc aatggcccac
     2761 aacgccggag acacgggagt ctcaggcgag tgagtgtcgc caggcttgct gagtccagag
     2821 ggccggtcaa tctccatcgt tcagaatttc caccagcatt cgtaggaatg ctttgagcgt
     2881 ctgcttatta gaatcaggaa agatagatga ggcacgaaat catcttatcc gccatgaaag
     2941 aagaagggcc agcgcgtaac actcccctgg ctctcgtgtt tttcccgaga aaaacggtcc
     3001 attctgatgt acctgctttt caaacggatt acattgaaac cttaaatacc tggcagtaat
     3061 ctttgtctca ttctccttcc ctcccaggag gtcccctatg cggataccgt tttcctcctg
     3121 cttctctgtt ttttcttctg tccacgcgca ccaattattt ctatcgtggt tcgtgctggg
     3181 cggtatattg tccggaagtc ctctctttgc cgctggctcg tttcaagaag actgtcttgg
     3241 aaaatcaccg gccggcatcg ccctgcagag ccatcttgtc gcggcgaaat ccggcaaggt
     3301 ggactccatg ttctgcgccg gagcgattca tctctacgtc caacatgatc tcaacaaggc
     3361 gctcccctgg ttggaaaaag ccaccaatgc cggcgacaag cgggcaccac ttgtcctggg
     3421 cattctctat taaaagggaa atggagttgc ggagaacccc gcc
//
LOCUS       DATIAL010000593        10248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_750985, whole genome shotgun sequence.
ACCESSION   DATIAL010000593 DATIAL010000000
VERSION     DATIAL010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750985"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VLA60_17890"
     CDS             <1..536
                     /locus_tag="VLA60_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11287.1"
                     /translation="ESEQQVLNAKMAKMLQTTDAASSSVSQPSLSTTDLTLEPAPYSE
                     IDGNREIQLTEREVNALIATDSYMARHVAVDMADDLVSVQLVVPIHHEMPIVGGKMLK
                     LNFGLELSYSNGKPVVAMRGISLGGIPLPSAWWGDIKNTNLVEEFGGSEGFWDQFSKG
                     VEDMKIQDGQLYVMLKE"
     gene            complement(930..1547)
                     /locus_tag="VLA60_17895"
     CDS             complement(930..1547)
                     /locus_tag="VLA60_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11288.1"
                     /translation="MWILLLILVLGGACSSQSVAQQKPSSPPVEQELQMAKTKSLYFI
                     VDTDKNIVLLKARGIPLRTFPLTGAEWIGPPLPHSTVLHLQTKDPSVSPLPISPPSDL
                     SQESPPEDPITPLTVSNMPTRYELTFQESLTILVQPPHLPSFWANLGHQIAGWGRRVT
                     ARMATWGGSHQYLVLSLDPAEAQALYWAAIPPMTCLVIPGNPADQ"
     gene            complement(1604..2242)
                     /locus_tag="VLA60_17900"
     CDS             complement(1604..2242)
                     /locus_tag="VLA60_17900"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSF11289.1"
                     /translation="MTSFSPPHSRQMLLLSLLVLLTAALGASPPHAPKRTPTVVPVTI
                     QPQEEHIPFYPSAPGLLATAPKGLYLVVDTAQNRVSLRKGNRILYSAVASTGSGARLQ
                     DPRNPDNGWVFDTPRGVFTISSKIKNPAWNKPDWAFIEEGQPIPTKSQDRIETGVLGD
                     YALGFGNGYFIHGTLYTRTLGTNVTHGCIRLGDEPLEYVFHHVPLGTTLIIY"
     gene            complement(2244..3455)
                     /locus_tag="VLA60_17905"
     CDS             complement(2244..3455)
                     /locus_tag="VLA60_17905"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HSF11290.1"
                     /translation="MITPPTRFQSAWRRRWMITLMLVLLPALTFARWGHTDYPDTFPS
                     LVEDLDRQAWVNGAETLFPDRYQQFHTHVLELRSQWRTEANHWWTTGDAEQFNQTYQQ
                     LVEEGSLLIEASRQKITALRLEVEELIQPEQAQLTRLRALSHDFDLEDDMLALSQSEA
                     LLRESVLRLEQGQYLQARSAGKQSIEHLRRVEAHVVTQMNRYTNEAQIARWEEWVTQT
                     LNRSVGTAVIVFKAPRRLLVYQHGRVIADYPARVGFSGLADKTYEGDGATPEGQFRVM
                     HKKKGPATIYYKALLLDYPTIAHQQRFDEGKANGLVPQNRSIGGLIEIHGEDPNNEES
                     TNGCIALENSAMDDVFERVKVGTPVTIVGALSQDNEVVTSLHQLEVHIQERNERWHKP
                     RTLAASFTRKE"
     gene            complement(3470..4135)
                     /locus_tag="VLA60_17910"
     CDS             complement(3470..4135)
                     /locus_tag="VLA60_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11291.1"
                     /translation="MTERIQINAGWKSLNGHMTRTVGLPWGKHVLFLTWLLLTGCAEP
                     PISQMNAATQALEDARISDAEHYAIERFTEAETAFRQVQQKLDRQGDRMPLFRNYDPV
                     ITMLSEVFNNATQAKIEAIANKKESKANAEVALAFAKQHLQDVRALLADISAPTPDHG
                     ELNQLLQAFQDTETLLAEIESIMAQEHFIDVMTTSHSVESFATRIQARIISVKRLAAK
                     QHV"
     gene            complement(4822..5601)
                     /locus_tag="VLA60_17915"
     CDS             complement(4822..5601)
                     /locus_tag="VLA60_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HSF11292.1"
                     /translation="MNTSGPHESYNLNIQTPAGDLTASVAVPTGFIPITDILPLMRSL
                     GEQAHQLEIDNTTQTGATISCQKGCAACCRMMIPVAPPEAFALLSVIEALPSPKKERL
                     LERFQAAQKTLREAGLEEGLQRLAFSEYQGTDEELEPLNRAYYALRMPCPFLEDEICS
                     IYEQRPSACRELLVTSPAELCQDLTRNPIQLIPSPFRIGTVLSKLWTDCYQGPVRLIP
                     LPFALDWATVHKSQNTRTWAGPDLLSRALDAAAQYLQRQSS"
     gene            6036..7487
                     /locus_tag="VLA60_17920"
     CDS             6036..7487
                     /locus_tag="VLA60_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HSF11293.1"
                     /translation="MLERVMERSVFRTKRFLNHPRSEDSHDLGLKRDLTVWHLVALGI
                     GAIIGTGVFVLIGTATVGNSHRPGAGPGIILSFLCSGMVCALAALCYAEFASMIPVAG
                     SAYSYTYATLGELIAWLVGWNLILEYGVASAAVAIGWSGYFVNVLKLVGIELPDLLAH
                     APEAGRGGLINLPAVLVSLVMMGLLLMGTKRSAQITGMIVVVKLFVILFFLAVGAGGV
                     NPENWTPFLPNGLSGVGAAAAIVFFAYVGFDAVSTAAEEARNPQQDIPRAILISLAFC
                     TLIYVSVAAVLTGLVPVTTIDVHAPVADALSRAGYRWGGALVAVGALAGITGVLLVMM
                     LGQIRIFFAMSRDGLLSPRLATIHSRYQTPSLSTMVTGSGVALLAGFIPIGEAADMTN
                     IGTLFAFCLVCLGVLWLRVTQPEHPRAFRLPWMPWIPVLGTLSCFGLMLFLPALTWIR
                     FGAWTCIGLLVYAFYGHRHSRLAATHKNISRFT"
     gene            complement(7491..8057)
                     /locus_tag="VLA60_17925"
     CDS             complement(7491..8057)
                     /locus_tag="VLA60_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HSF11294.1"
                     /translation="MAQSPTSPDSPIGSTPEDIPHWIEHVFEMGIFASRWIQAPLYGG
                     LILAEVLYAWKFLTELWHMVHEIGTLTETVFMLGILTLVDITMVANLLTMVVIGGYAT
                     FVSKISLESHPDRPDWLSHIDPGTIKVKLAASLIGISSIHLLKAFVNIANVPIEHIQW
                     QIIIHLTFLGSAVLLAWTDKIMTKAKNH"
     gene            complement(8509..>10248)
                     /locus_tag="VLA60_17930"
     CDS             complement(8509..>10248)
                     /locus_tag="VLA60_17930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HSF11295.1"
                     /translation="AIRTTIQSVKDVQFAGRVLVDPQSKQPVLYTENLFAKFDDDLAE
                     STCRKILKASGLGIKRVLEYARNTFFIEAPEGTGQEVFAIAESLLRHKHVELCHPELV
                     RQMGWRTAFPRQWHLKKTTIDGTVYDAHANVEAAWALSEGENITVAIIDDGMDLDHEE
                     FAGSGKLIAPRDVTRATDDPRPGSRDNHGTACAGVATGNGFHGASGVAPKARLMAIRY
                     VSPLGSQAEADAFIWAAQHGADVISCSWGPEDGAWWDPRDPLHHTVVPLPDSTRLAID
                     WAVANGRNGKGCVITWAAGNGNESVDNDGYASYEKVIAVAACHAKGKRSAYSDFGKAI
                     WCTFPSNDTILPIPGIWTTDRSASAGYNPGQVSRGDAAGNYVNDFGGTSSACPGVAGV
                     AALMLARNPSLRWNEVKDLLRRSCDQIDPSAGKYDTNGHSTLYGYGRINAKRAVELAA
                     PAIPTPTTVHTSTAIIPIRDLQTSQLSLQVAETTPLTSVKIGVDIEHSYIGDLIIKLV
                     PPNSTGVSSIVLHNRIGGGTNNIHTTYDVTTTPDLLAFVGKIPKGKWSLTVQDKEKLD
                     TGRILQFSVTLVF"
BASE COUNT         2242 a         2397 c         2941 g         2668 t
ORIGIN      
        1 ctgaatcgga gcagcaggtc ctgaatgcca aaatggcgaa aatgcttcag accaccgatg
       61 ctgcttcttc atctgtgtcc caaccttcct tgtccacaac agacctcacc cttgagccgg
      121 cgccgtattc tgaaatagat ggaaatcgag agattcagtt aaccgagcgg gaagtgaatg
      181 ccttgattgc cacggattcg tatatggcca gacatgtggc tgttgatatg gcggacgatc
      241 tcgtcagtgt ccaactggtg gtcccgattc atcacgagat gcccatcgtg ggtggaaaga
      301 tgttgaaact taattttgga ctggagctca gttattccaa tgggaagcct gtggtggcga
      361 tgcggggtat tagtctcggt ggaattccac ttcccagtgc ctggtgggga gatattaaga
      421 ataccaacct ggtggaagaa tttggtggct cagaaggatt ttgggatcaa ttttcgaagg
      481 gtgtcgagga tatgaaaatc caggatggtc aattgtatgt catgttgaaa gagtagtcgt
      541 ccccatttcc agcgcaagcc tgttcattcg cttgaaactt gcttgatttt caaacatctc
      601 agaaacgtag cctatcctcc ttcgactttc gcgccgctca aagcgaaaag gaatttttca
      661 ccccggtgct ggtcatctcc agccgtttgg gctgaagtgc tccctgccga cttggagttc
      721 cccttctccg gttgtttttt attatgtccc cgtcgccctt tttagcttgt attctatctt
      781 caatccttcg gatagtattc cccctcttcc tgtaccctgc ccagggcagt tgggagattc
      841 cgagctttca tgtttttggc gatacagttg gcgaatcttc agaccctggt aggaaacgaa
      901 ccattgatcg ctgaatgaga aaacgggttt tattggtctg cgggatttcc cggaatcacc
      961 aggcacgtca taggcggaat ggcggcccaa tacaaggctt gcgcttcggc aggatcaagt
     1021 gagagcacca ggtattggtg ggaaccgccc caggtcgcca ttctggctgt gacccgtcgc
     1081 ccccagccgg ctatctgatg ccccaggttt gcccaaaagg aggggaggtg agggggttgg
     1141 accagaatgg tcagggattc ttgaaaggtg agctcatagc gagtgggcat gttgctgacg
     1201 gttaacggag tgatgggatc ttccggcgga gattcctgag ataaatctga tggaggagaa
     1261 atgggtaggg gagagactga tgggtctttc gtctggaggt gcagtacggt tgagtggggg
     1321 agaggaggtc cgatccactc ggcgccggtc agaggaaatg tccgcagggg gatgccacgg
     1381 gctttgagca atacgatgtt cttgtcggta tcgacgatga aatagagaga tttggttttt
     1441 gccatttgca gctcttgttc aacgggagga gaagagggtt tttgttgagc cacgctctgg
     1501 gaagaacagg cccctcccag gacaaggatg aggagaagta tccacaccaa gtaggagctg
     1561 gtggtttgga gtggccagtg gatgaagcaa gccttgagag gattcaatag atgatcaggg
     1621 tggtgccaag gggcacgtgg tgaaagacgt attcaagggg ttcatctccg agtctgatgc
     1681 agccatgggt gacgttggtg cccagggttc gcgtatacag cgtgccatgg atgaaatagc
     1741 cattgccgaa accaagggca tagtctccca gcacgcccgt ttcaatccgg tcttgggatt
     1801 tggttggaat gggttgccct tcttcaatga aggcccaatc gggtttgttc caggccggat
     1861 ttttaatttt actggaaatc gtaaacaccc cacgcggagt atcgaacacc caaccgttgt
     1921 cgggattgcg aggatcttgc aatcgtgccc cgctgccggt tgaagccacg gcactgtaga
     1981 gaattcgatt gccctttcga agggataccc gattttgagc cgtatcgacc acgaggtaga
     2041 ggcccttggg tgccgtggca aggagaccgg gagcggacgg ataaaacggg atgtgttctt
     2101 cctggggttg gatcgtcaca gggaccacgg ttggggtgcg ttttggagcg tggggaggtg
     2161 aagcaccaag tgccgctgtc aggaggacga gcaggctcag cagtagcatc tggcgtgagt
     2221 ggggagggct gaaggacgtc attttattct ttgcgggtga atgaggcggc aagcgtacgg
     2281 ggtttgtgcc accgttcgtt ccgttcctga atatgcactt ccagctggtg taaggaggtc
     2341 acgacctcat tgtcctggct cagcgcccct acgatggtga caggcgttcc gacttttacc
     2401 cgttcaaata cgtcatccat cgcggaattt tccaaggcaa tacacccgtt agtggattct
     2461 tcgttattgg gatcttcgcc atgaatttca atcaggccgc cgattgaccg gttctggggc
     2521 accaggccat ttgctttacc ctcgtcgaaa cgttgttggt gggctatcgt ggggtagtcc
     2581 aagagtaacg ctttataata gatggtcgcc ggtccctttt ttttatgcat cacgcgaaat
     2641 tgtccttccg gtgtggcgcc gtctccctcg taggtcttgt cggccaatcc cgaaaatccc
     2701 acccgtgccg gataatccgc aatgacccgg ccgtgttggt agacgaggag gcgacgaggg
     2761 gccttgaaga caatgacggc tgtgccgact gatcggttta gggtctgcgt gacccattcc
     2821 tcccagcggg caatttgggc ttcgtttgta tagcggttca tttgtgtgac gacatgggct
     2881 tccacccggc gaagatgttc aatcgactgc tttccggccg accgggcctg caggtattgt
     2941 ccctgctcta atcgcaacac actttctcgg agcaatgcct ctgattgaga cagtgccagc
     3001 atgtcgtctt ccaggtcgaa atcatgagaa agcgctcgca accgtgtgag ctgcgcctgt
     3061 tcaggctgaa tgagctcctc gacctccagg cgcagggcag tgattttttg gcgagaggct
     3121 tcaatcagca gagagccttc ttccaccagt tgttgataag tctggttgaa ctgttcagcg
     3181 tcgccggttg tccaccagtg attggcctct gtccgccatt gggaacggag ttcgagcacg
     3241 tgtgtatgaa attgttgata tcgatcagga aatagggtct ctgcgccgtt gacccatgcc
     3301 tgtcggtcca ggtcttcgac tagtgaagga aaagtgtcgg gatagtctgt gtgtccccac
     3361 cgggcaaacg tcaacgctgg gagcaggacc agcatgagcg tgatcatcca gcgccgacgc
     3421 caggccgact gaaacctggt tggcggagtg atcatagtat tctccattat cagacgtgtt
     3481 gctttgcggc aagccgtttg acagagataa tccgtgcttg aattcgagtg gcaaaagatt
     3541 ctacggaatg ggaggtggtc atcacgtcga tgaagtgttc ttgtgccata atggattcga
     3601 tttcagcgag gagggtttcc gtgtcctgaa aggcttgtag aagttggttc agctccccgt
     3661 gatcaggcgt cggagccgaa atgtctgcca ggagcgcacg aacgtcttga agatgctgtt
     3721 tggcaaaggc gagagccacc tcggcattgg ctttggactc ctttttattg gcgatggctt
     3781 cgattttggc ctgcgtggcg ttgttgaaga cttccgacag catggtgatg acaggatcat
     3841 aattacgaaa aagcggcatc cgatctccct gtcgatccag tttttgttgt acttgtcgaa
     3901 aggccgtttc tgcttcagtg aatctctcga ttgcgtaatg ttcggcgtct gatattctcg
     3961 cgtcttcaag tgcttgggtg gctgcgttca tttgggagat aggtggttct gcgcaaccgg
     4021 tcaagaggag ccaggtcagg aaaagcacat gtttgcccca gggaaggcca actgttcgag
     4081 tcatgtgccc gttgagggat ttccagccag cgttaatttg gattctttca gtcacgatga
     4141 ttgagatatc cctctcagtc cggaaggtcg tttttcttta ggaggaggag gggccgaacc
     4201 attccggccc ctcttgttgt tcgcccgaat caaaacctga acaggtgggg ccttttcctt
     4261 gatggaaaaa ggtcgtgaat tgcaggtatc gggtatcgtc cggataccta tttgcctagt
     4321 ggctttttgg ccggattttg ctgtggctta gcagggctcg gggcggcagt cggtttcccg
     4381 attggcatac cctttttggg gtcttttgga tttgattggt tcacagtagc acctccttta
     4441 aaggttaggt aagccgtcat ggcttactac tcttaggtat agcagcaaag gtaatgccag
     4501 attttgatct gctgtgcagg aggaattcct gcatttttat gagttaatgc ggtgaaaatt
     4561 cttgcaagaa cctcatgcgc agagacatcc tgtccaatga aagaaatctt gtccattttc
     4621 cctgaaaatt ctggttcaga tttggagtgt ctctgtaaaa tattatccgg cgtttcgatg
     4681 gaggtccggg attggtttct atcttaaagg cagaggtttg atcccgcttc caccattctg
     4741 gtgccggtgc tggttgatgc caggtaggcc tgcacgtagg acctttgatt cgtgtatgaa
     4801 acaggagggg atgtgtgcct cttatgagga ttggcgttgt aaatattgcg ctgcggcatc
     4861 caaggcccgg ctcagcaggt caggcccggc ccaggttcgg gtgttctggc ttttatggac
     4921 tgtcgcccag tccagtgcaa atgggagggg aatgagacga accgggccct ggtaacaatc
     4981 cgtccaaagt ttgctcagaa ccgtcccgat tcgaaacggg gaaggaatca gttggatggg
     5041 gtttcgtgtc aagtcttggc aaagttccgc aggggaagtg accagcaatt cccggcaggc
     5101 ggacggccgc tgttcataga ttgagcagat ttcatcttcc agaaatggac aaggcattcg
     5161 aagcgcatag tacgcacgat taagaggctc cagttcttcg tcggtgcctt gatattcgga
     5221 aaaggccaat cgttgcaacc cctcctccag gccggcttca cgcaaggtct tttgagccgc
     5281 ctgaaatcgc tcaagcaatc gctctttttt gggagaaggg agtgcctcaa taaccgacag
     5341 gagcgcaaag gcctccggtg gggcgacggg aatcatcatg cgacagcagg cggcgcagcc
     5401 tttttgacag gaaatggtgg caccggtttg agtggtgttg tcgatttcca actggtgagc
     5461 ttgttctccg agggatcgca tgagggggag aatatcggta atgggaatga atccggtcgg
     5521 taccgcaacc gacgccgtca gatctccggc aggggtttga atattcaggt tgtaggattc
     5581 gtgaggtccg gaggtattca tggaaaaatc atatgaagga acaatcaaat aaacagatta
     5641 cgttaataga cgtcactgcc cggccgtgac atgcactgga ggtgagccgg agattgaatt
     5701 gtagaagaaa gcgtatgagg aggaaaagct gatggcttgg gttcacctga catgcggggg
     5761 agggcatgat tttctgcatg acgccattcc aggagaatca tgggtcttac aagattcccg
     5821 gccttttcct tgagagtttc atttcttgag gaagaaaaaa ttcatataca agtccttctg
     5881 tttcaaatgg gcttcttgta tccagggggc tgacccccgg cacatttata gcatagtcgg
     5941 gtcgtggtta gggattgaag atctcttttt tcttacccgg ttctcccctt aaggcataaa
     6001 aaccgcatct tggaaaggaa agtcgaacca aaacgatgct tgaacgtgtg atggaacgtt
     6061 cagtatttcg gacgaaacgt tttctgaacc acccgcgttc cgaggattcc catgatttag
     6121 ggttaaaacg ggatctgact gtgtggcatt tagtcgcgct tgggattggc gccattattg
     6181 ggaccggagt gtttgtctta ataggaacgg ccacggtcgg caattcccat cgaccgggtg
     6241 cagggcccgg catcatcctg tcctttttgt gttcgggaat ggtctgcgcg ctggctgcgc
     6301 tctgttatgc ggaatttgcc tctatgattc cagtggccgg cagcgcgtat tcctacacct
     6361 atgcgacgtt gggagaattg attgcctggt tagtgggttg gaatctgatt ttggaatatg
     6421 gcgtggcgag tgcggctgtg gccattggct ggtccgggta ttttgtcaat gttctcaagt
     6481 tggtgggcat tgaactgccg gatctgctgg ctcatgctcc agaagcaggc cgcggtggcc
     6541 tgatcaatct tccggccgtg ctcgtctctc ttgtgatgat ggggctactc ttgatgggaa
     6601 ccaagcgaag tgcacagatc accggtatga ttgtggtcgt gaaacttttc gtcattcttt
     6661 tctttctggc tgttggagca ggaggggtga accccgagaa ttggaccccg tttcttccta
     6721 atgggctatc cggcgtcgga gcagccgcag ccattgtgtt tttcgcgtat gtgggtttcg
     6781 atgcggtctc gacggcagcc gaagaagctc gaaaccccca gcaggacatt cctcgggcta
     6841 tcttgatatc cttggcattc tgcacgctta tttatgtctc cgtggcagcc gttctgacgg
     6901 ggttagtccc tgtaacaacc attgatgttc atgcacctgt ggcagacgct ctcagccgag
     6961 caggataccg gtggggcgga gccctggttg ccgttggggc tttggccggg attaccggcg
     7021 tattacttgt catgatgctt gggcaaatcc ggatcttctt tgcgatgtcc cgagatggtc
     7081 tcctcagtcc tcgcctggcc acgattcatt ccaggtatca gactccgtcc ctgtccacca
     7141 tggtgactgg atcaggggtc gcgcttctcg ctgggtttat ccccatcgga gaagccgctg
     7201 atatgacgaa tattggaacc ctttttgcct tttgtcttgt ctgccttggc gtgctgtggc
     7261 tccgtgtcac ccaaccggag catccacggg cgttccggct tccctggatg ccatggatcc
     7321 ccgtgttggg aacactatcg tgttttgggc ttatgctctt tctccccgca cttacctgga
     7381 ttcgttttgg ggcgtggaca tgtatcggac tcctggtcta cgccttctat ggccatcgcc
     7441 acagccggtt ggccgccact cataaaaata tttcgagatt cacatagcgg ttaatggttc
     7501 ttcgccttgg tcatgatttt atcggtccag gctaacaaca cggcagagcc taaaaaggtc
     7561 agatggatga tgatttgcca ttgaatgtgt tcaatgggca cattggcaat gttgacgaag
     7621 gctttaagta aatgaatgct ggaaatgcca atcaacgagg ctgcgagttt cactttgatc
     7681 gtgcccgggt caatgtggct gagccaatcc ggtcgatcag gatgactctc cagacttatt
     7741 ttactgacaa atgtcgcata tcctccgatg acgaccatgg tgagcagatt ggccaccatg
     7801 gtgatatcca ccagggtgag gattcctaac ataaacacgg tttcggtgag ggtaccaatt
     7861 tcatgtacca tgtgccacag ttcggtcaga aatttccagg catagagcac ttcagcgagg
     7921 atcaatccgc catataaggg tgcttgtatc caccggctgg caaaaatgcc catttcgaat
     7981 acgtgttcga tccaatgggg aatgtcttcc ggtgtggaac cgataggaga atccggtgaa
     8041 gtcggggatt gggccataaa aacctccttt gaaaaaatgt tgaatcaacg cgtaaagctc
     8101 tccagtctat ccgggaacat gaccgtgtta ggaacgcatg cgaacaatgc tctttaaacc
     8161 ttaaaaaaag tgatcgttgg cacagaattt gatgtgagaa aaaaggaagg cacatcattc
     8221 caagttgatc aattactgtt ggtaggtaag aaattttatg agtgaggagg aatgatcaag
     8281 attggtacgg aaggtgggtg agcacgaaat tgaagcgaag ggatgaaatt gtgttcttga
     8341 ggtggtcagt ttggcaaata tactgggagc gtgaaaacag atcaagtttt ctaagatggt
     8401 ctccggtgag gagttctatt ttcatatcag atggcatcgg ccacggacat atcagacggt
     8461 ggccgataca tgcatacgct ctcccacacc ccatgccggt caacttcctt agaaaaccag
     8521 cgtcaccgag aactgtaaga tgcgcccggt atcaagtttc tctttatcct gaaccgtcag
     8581 tgaccatttt ccctttggaa ttttgccgac gaatgccagg aggtccggtg tcgtggtgac
     8641 atcataggtg gtgtggatat tatttgtgcc gcctccaatc cgattatgaa gcacaatgga
     8701 cgatactcct gttgaattgg ggggtaccag cttaataatt aaatcaccga tatacgaatg
     8761 ttcaatatcc actccgatct tgacggaggt gagcggggtg gtctcggcca cttgtagaga
     8821 gagttgtgag gtttgaaggt cgcgaatggg aatgatggcg gtggaagtat ggacagtggt
     8881 aggcgttgga atggccggtg cagccagttc taccgcgcgt ttggcattga tccggccata
     8941 cccgtataag gtgctatggc cgttggtgtc atatttgcca gcgcttggat caatttgatc
     9001 acatgagcgt ctgagcagat ctttgacttc attccatcgc aaagaggggt tgcgagcgag
     9061 catgagggcg gccacccctg cgacaccggg acatgcgcta gatgtgccgc caaaatcgtt
     9121 gacataattg cctgccgcat cgcctcgact gacttgtccg ggattatatc ctgctgatgc
     9181 tgagcgatcg gtggtccaga ttcccggtat ggggagaatc gtatcgttgc tgggaaaggt
     9241 acaccaaata gccttgccga aatcgctata ggcacttctt ttccctttgg cgtgacaggc
     9301 ggccacggca atcacttttt cataactggc ataaccatcg ttatcgacac tttcgttgcc
     9361 gtttccggcg gcccaggtaa tcacacaccc ttttccgttg cgtccattcg cgacggccca
     9421 atcaatagca aggcgagtgg aatccggcag gggcacgacg gtgtgatgga gcggatcgcg
     9481 tggatcccac caggcgccat cctccggccc ccagctacag gaaatgacat ctgcgccatg
     9541 ttgggcggcc cagataaatg catcagcctc ggcttgtgag cctaacgggg atacataacg
     9601 aatggccatc aatcgggctt tgggagcaac accggaggca ccatgaaaac cgttgccggt
     9661 ggcgacgccc gcacaggcgg ttccatgatt atcccggctg ccgggtcgag gatcatcagt
     9721 ggcgcgtgtt acgtcgcgag gagcaatcag ttttcctgaa ccggcaaact cttcgtgatc
     9781 aagatccatg ccgtcatcaa tgatggcgac agtgatattt tctccctcac tgagtgccca
     9841 ggcggcctcc acgttggcat gggcatcata gaccgttcca tcaatcgtgg tctttttcag
     9901 gtgccactgt cggggaaatg ctgttcgcca tcccatttgt ctgacaagtt cagggtgaca
     9961 gagctccaca tgtttatgcc gcaagagact ctcggctatg gcaaatactt cctgccctgt
    10021 gccctccggc gcttcgataa agaaggtatt gcgagcatat tccaataccc gtttgattcc
    10081 gagtccggag gcttttaaga tttttcgaca agtggattcg gccaggtcat catcgaattt
    10141 agcaaaaaga ttttcggtat atagaacagg ttgttttgat tgaggatcga ccaggacccg
    10201 tccggcaaat tgtacgtctt ttacggattg tatggtggtg cggatggc
//
LOCUS       DATIAL010000594         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1072694, whole genome shotgun sequence.
ACCESSION   DATIAL010000594 DATIAL010000000
VERSION     DATIAL010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072694"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VLA60_17935"
     CDS             complement(<1..464)
                     /locus_tag="VLA60_17935"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF11296.1"
                     /translation="MQILRQKIQWKVWIILATLSFACASAWAGSAGDLQPRVPSDRME
                     QALSFTNPFTPTEEFIASGKKLYEGKGWCAFCHGWEGTGAPTAGRTFPPDIKMPTNFA
                     DAAWQAARSDGELFWVLIHGSHGTDMVAYMPQYITDKEAWQIIAYLRTFGGT"
     gene            complement(966..2252)
                     /locus_tag="VLA60_17940"
     CDS             complement(966..2252)
                     /locus_tag="VLA60_17940"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HSF11297.1"
                     /translation="MTEPTIICPQCKTEIKLTESLAAPLLEATKRDFEQRLAQKDADA
                     AKRDAALREREALLAKSQERFEEQVADKLKLERSKIVAEEARKARLALSNDLDQKSKE
                     IHEIQELLKQKDEKLAEAQKVQADLLRKQRELDDAKRELDLTIEKRVQEGLTATRDQA
                     KKEAEEGLKYKVMEKEQTIASMQKQIEELKRRAEQGSQQLQGEVQELELEAMLTSKFP
                     LDQISPVAKGEHGGDVLHRVASSFGQACGTILWESKRTKNWSDGWLIKLREDQRQAKA
                     EIAIIVSQALPKEVETFEFIDGVWVTHPKVALPLAIAMRNTLMEVAGARQASEGQQTK
                     MEMVYQYLMGPRFRQRVQAIVEGFSSMKEDLDKERKVIMKQWAKREEQIDRVMMATVG
                     MYGDLQGIAGKTLQEIEGLELQALDAPKDESDGKLL"
     gene            complement(2334..2822)
                     /locus_tag="VLA60_17945"
     CDS             complement(2334..2822)
                     /locus_tag="VLA60_17945"
                     /inference="COORDINATES: protein motif:HMM:NF013729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HSF11298.1"
                     /translation="MKKNITVFVSYARANRDLATRLLKKFTEQAAPSKQYHYTFWRDS
                     DILVGEKWHEEIQHALGECDVGLVLISPALLGSQYIQDHELPKFVKSGGKSVIPVMLQ
                     PIDLERHDLKGLLQTQIFRLDRPRFASPKAYGECSGSQRDQFALELFRQVEARLDKIV
                     VK"
     gene            2977..3669
                     /locus_tag="VLA60_17950"
     CDS             2977..3669
                     /locus_tag="VLA60_17950"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HSF11299.1"
                     /translation="MTQKILLIGNSDGIGAAVTRALIARGEHVVGLSRSPSPLGPDGP
                     RHIVQDITAPEYPQVLATLLADDGPFDACIFCAAIGSNLTLPDLSQEARVIDVNFTSM
                     VRTLAALAPGWLERRQGHFIGLSSLADDFYNADSPSYTASKAGFSNYLVSMGLSLRSH
                     GVYVSNIRFGFVDTKLPKASWKPLMMTADQAAGHVLRCLETKPMQLSVPKFAGLTIHG
                     IRWMQSIRIWWA"
     gene            3737..4276
                     /locus_tag="VLA60_17955"
     CDS             3737..4276
                     /locus_tag="VLA60_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HSF11300.1"
                     /translation="MTLRAHTFLKGAFPDDRNTSFRRYSCGNHSPLGPVSSLAGTWEG
                     DRGVDIAPAKEGSHETHFRERLQFDPIGPVVNGSQVLYGLRYSTVAWPLIQEQPFHEE
                     AGYWLWDAVRQQVMQCFMVPRGVLEATDDDRRSGRGPCPTMLGNKTHATERPQISRPR
                     HSRYPLDAINSNMVGLNTG"
BASE COUNT          954 a         1171 c         1058 g         1117 t
ORIGIN      
        1 tatgtccctc cgaacgttcg gagataggcg ataatctgcc aggcctcctt atccgtaata
       61 tactgcggca tgtatgccac catatcggtc ccatgactcc catgaatcag aacccagaaa
      121 agctccccat cacttctggc tgcctgccag gccgcatctg caaaattggt aggcattttg
      181 atatcaggag gaaaggttcg acccgcagtc ggcgcccctg tcccctccca gccatgacaa
      241 aaggcgcacc agcccttgcc ttcatagagt ttttttccgc tcgcaataaa ttcctctgtg
      301 ggggtaaagg gattagtaaa ggataaggcc tgctccatgc gatcggatgg gacccgtggc
      361 tgaagatctc cggcactgcc cgcccaagcc gaggcacagg cgaaagaaag cgtggcaagg
      421 atgatccaga ctttccattg aatcttttgc ctcaatattt gcattaggac tgtctccttt
      481 gttggtgata taggcctggc tcttgcccct gtctcaataa gactctccaa attacgtgcc
      541 agtataaaag tggtattttc acaaggtttt cctctttcaa gcatgaaagt gaggcaattt
      601 gcatcattcc ataaacagcg caggtgccca aatttcccca aaccaatgtc gtgtggtgtc
      661 aggccacaat ccgtacgaaa ctgaatcgga acctcttcca tccagccatg atcttttttt
      721 taaggacgcg gtttggcttt acaggcgaac ctattacggg aaaggctagt cccctagaac
      781 cgaaggcaaa aactagagaa ggcaggagcc ttgtgttgat gtaaagatga gaaatgttga
      841 tggatggcag aatgactcag gagaattgtg gcaccttgct gtaagacccc ggagaatgag
      901 tcgatacggg cctgatcatg ctaagaccaa gcctttgctt atgaaggaag cagaggcgga
      961 agttcttaca acaattttcc gtcggattcg tccttgggcg cgtctaaagc ctggagttcc
     1021 agcccttcaa tttcctgcaa cgtttttccg gcgatgcctt gcaggtcccc atacatgccc
     1081 acggtcgcca tcatcacccg atcaatttgc tcttcacgtt tagcccattg cttcatgatg
     1141 actttccgct ctttatcaag atcctccttc atcgaggaga acccttcgac gatcgcttga
     1201 acccgttgcc ggaagcgcgg acccatcaaa tactgataga ccatttccat tttggtttgt
     1261 tgcccctccg aggcctgcct ggcgccggcc acctccatga gcgtattacg catggcaatg
     1321 gccagcggca gggcgacttt gggatgggtg acccagacgc cgtcgatgaa ttcgaatgtt
     1381 tccacttctt tgggtagggc ctggctgacg atgatcgcga tttcagcttt ggcttgccgc
     1441 tgatcctcac gtaacttgat gagccagcca tcactccagt tcttggttct cttcgattcc
     1501 cacaagatgg ttccgcaggc ttgcccgaat gagctggcaa cccggtgcag cacatcgccg
     1561 ccatgctcgc ctttggccac gggtgatatc tgatccaggg ggaatttcga ggtgagcatg
     1621 gcttcgagtt cgagctcctg gacttcgccc tgaagttgtt gagacccctg ttcagcacga
     1681 cgcttgagct cttcaatttg tttttgcatg gacgcaatgg tctgctcttt ttccatgact
     1741 ttatatttga gcccttcttc ggcttctttt ttggcctgat ccctggtagc agtgagtccc
     1801 tcctggacgc gcttttctat agtgagatcc aattcgcgtt tggcgtcatc cagttcccgt
     1861 tgcttacgga gcagatcggc ttgaactttt tgtgcttccg caagtttttc atccttttgt
     1921 ttaagaagtt cctgaatctc atgtatctcc ttagatttct ggtcgagatc attcgagagc
     1981 gccagcctgg cttttctcgc ttcttccgcc acaattttgc tgcgttcgag tttcagcttg
     2041 tcagccacct gctcttcgaa cctttcctgt gatttggcca gcagagcttc gcgctcacgc
     2101 agggccgcat cccgcttggc ggcatcagca tctttttgag caagtcgttg ctcgaagtcc
     2161 cgcttcgtgg cttcgagaag aggggccgcg agcgactcag tcagtttgat ttccgtttta
     2221 cactgtgggc agatgatcgt gggttcagtc atagaatttc ttgttgctct tcaattgaat
     2281 aatagaaata cctttcggag ggataggacc tgaagactcc atcctcccaa ggattatttg
     2341 acgacgattt tatccaaacg ggcttcgacc tgccgaaaca attccaacgc aaattgatca
     2401 cgctgcgaac ccgaacattc tccgtaggct ttcggagagg cgaaacgggg gcgatccagc
     2461 cgaaagattt gcgtctggag taaacctttc agatcatgac gttccaaatc gatgggttgc
     2521 aacatgaccg gaatgacgga tttaccgcca ctcttcacaa acttgggaag ttcgtgatcc
     2581 tgaatatatt gggacccgag caacgcaggg ctgatcagga ccaaacccac gtcgcactct
     2641 cccaaggcat gttgaatttc ttcgtgccat ttttcgccta ccaggatatc gctatcccgc
     2701 cagaaggtat agtgatactg cttggagggc gcagcctgct ctgtaaactt tttcaacaat
     2761 cgggtcgcca ggtcacgatt ggcgcgggca taggacacaa acacggtaat gttctttttc
     2821 atactcgtgt aagcagaagc ggccgatctg tcgaaccatc ctagccaaca catgcaacaa
     2881 tgtacaggtc agggtcttgc tgattgttcc ctcttcactt acgccctttt tcatgtcagg
     2941 attgaggctt cacccaccat ctcggttggg ctttgaatga cacagaagat tctcctgatc
     3001 ggcaattctg atggaatcgg agcggcggtg acccgtgccc ttattgcccg tggcgaacat
     3061 gtcgtcggac tttcaagaag ccccagtcca cttggtcccg atggcccacg gcatatcgta
     3121 caggatatca ccgctcccga atatccgcag gttctagcaa cccttcttgc ggacgatggg
     3181 ccttttgacg cctgcatatt ttgcgcagcc attggctcaa acctgacgct tcctgacctc
     3241 tcacaggaag cccgcgtcat tgacgtgaat ttcacgtcga tggtgcggac cctggccgcc
     3301 ctcgcccccg gctggcttga gcgaagacag gggcacttta tcggcctatc aagtctcgcc
     3361 gatgattttt acaacgccga ctccccttcc tatacggcat cgaaggccgg ctttagtaat
     3421 tatttagtct ccatgggatt atcgctccga tcgcatggcg tgtacgtgag caatatccgc
     3481 tttggctttg tggatacgaa gttgcccaag gcttcctgga agcccctgat gatgaccgcc
     3541 gatcaagccg caggccatgt cctacgctgc ttggaaacaa aacccatgca actgagcgtc
     3601 cccaaattcg caggccttac cattcatggt atccgttgga tgcaatcaat tcgaatatgg
     3661 tgggcctaaa cacgggttaa acaatgggaa agatttgata cagtcgtgaa cttcagatta
     3721 cccgtcactt cgcctcttga ccctccgtgc tcacactttc ctgaaaggcg cctttcctga
     3781 tgaccggaac acatcattcc gacgctactc atgcggaaat cattcgccac ttgggcccgt
     3841 ttcttctcta gccggaacct gggaaggcga taggggtgtc gatatcgcac cggccaaaga
     3901 gggcagccac gaaactcatt tccgggaacg cttgcagttt gatccgatcg gaccggtcgt
     3961 caatggctcg caagtgctct atggtctccg ctactccact gtggcctggc cattaattca
     4021 ggaacagccg tttcatgaag aagccggata ttggttgtgg gatgcggtca ggcaacaggt
     4081 gatgcagtgt tttatggtcc ctcgaggcgt cctggaagcc actgatgatg accgccgatc
     4141 aggccggggg ccatgtccta cgatgcttgg aaacaaaacc catgcaactg agcgtcccca
     4201 aattagcagg cctcgccatt cacggtatcc gttggatgca atcaattcga atatggtggg
     4261 cctaaacacg ggttaaacaa tgggaaagat ttgatacagt
//
LOCUS       DATIAL010000595         3122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1180738, whole genome shotgun sequence.
ACCESSION   DATIAL010000595 DATIAL010000000
VERSION     DATIAL010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180738"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..589)
                     /locus_tag="VLA60_17960"
     CDS             complement(131..589)
                     /locus_tag="VLA60_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF11301.1"
                     /translation="MNTSYVPDTTQRTAGMFSGQSFRGGVLVVDDEGAIRKVVRMALE
                     KDGYYVVDAEDGAQAIRILNEGEHPMVIDVIITDIRMPNINGIEAIKYFQREYPSVAL
                     MVLTGFPDIDLATQLMKQGITDYLVKPIDRKKLQTGVADAIASRHLNWFS"
     gene            complement(990..1775)
                     /locus_tag="VLA60_17965"
     CDS             complement(990..1775)
                     /locus_tag="VLA60_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HSF11302.1"
                     /translation="MNQYIFDNKSEEREFQRLQLVEAANDPTTIRLLEETGIQPGWHC
                     LEVGAGAGSILRWMGSQVGPTGLAVGVDKKIAYLHKDDSPHFQIYQGSILDVPIEQSF
                     DLLHSRYVLIHNKEEGAILRKLSSLLKPGGWALFEEPDFTSATLQDRKQETPQGMVNR
                     AICQMFINLGLDPAYALRLPHKLQEAGFHIVKVQSLMHLCPGNSPMARVMAESALVLE
                     QEYLKTGLCSPKDIQVYVRLSQDSTHWALYHSTTSVIARKPNI"
     gene            complement(1775..3046)
                     /locus_tag="VLA60_17970"
     CDS             complement(1775..3046)
                     /locus_tag="VLA60_17970"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HSF11303.1"
                     /translation="MSSDIDIAIIGAGAAGLAAGIFAAETNPNLSIALLDSAKTIGAK
                     ILVSGGGRCNVTNQRVTASDFHGNRKLIDRILRRFDEQATIRWFSSLGIPLKTEATGK
                     LFPESNKARTVLNGLLRRCDALGIALLTHHLVRDLTREGEEFSIQHSQGTLRVKRVIL
                     ATGGRSLPKTGSDGSGWRLAQQLGHTVTATYPALVPLLLDPAFFHATLSGISHDVTLT
                     TTIAGKTFDRRTGSLLWTHFGISGPVVLDASRVWVRAAAKGEAVHLHLHCFPHLSNQD
                     VEKWLMQAASLPGRKLVPTLLAERLPTRVAGTLASIQEPGLSDTPVNLLSKEARRDLV
                     RTLTNLDLPVIGSRGWNFAEVTAGGVPLEEISAHSMGSRQVPGLYMIGEMLDCDGRIG
                     GFNFQWAWATGYLAGCHAALDIMKHAAAEEG"
BASE COUNT          751 a          768 c          842 g          761 t
ORIGIN      
        1 gccaaaagag aaaaagaatg aacaggaaga ggcgatgcca aaaatcgtcc tcttcgaatt
       61 cctgtctctg cccagagggg tgagtggaag tcttttcctc gagaccaccc aaaataaggc
      121 aaaaaccacc tcaagaaaac cagttgagat gtcgtgaggc tatggcatcg gccacaccag
      181 tttgtaattt ttttcgatcg atcggcttga ctaaataatc ggttattcct tgtttcatga
      241 gctgcgtggc tagatcgata tctggaaacc cggttagaac cattaacgcc acgctggggt
      301 attcccgttg aaaatatttt atggcttcaa tgccattgat attgggcatc cgaatatccg
      361 tgataatgac atcaatgacc attggatgct ctccttcgtt gaggatgcgg atagcttgcg
      421 cgccgtcttc tgcatccacc acgtagtagc cgtctttttc caaggccatt cgaacaactt
      481 tgcgaatggc gccttcatca tcaacgacca ggactcctcc ccgaaagctc tggccggaaa
      541 acattcctgc tgttcgttga gtggtgtcag gtacgtagct tgtgttcatg gcgaatcctc
      601 cttgcctatg cagtgaaatg gtatctctgc gcgaaaaaaa aacatccgaa tccttctgta
      661 tggtatgagg cattcctaat ctcagtatct tgcaaccagt gtgccttgat gggctgataa
      721 gggactatgg ccctctcttg gaaaacagtc ctgaaaatta ataggaaaaa tggaaagaat
      781 gattggagtg tgtatgacca ggaaggagag acggtcagtg aagatgtcta cagaggattc
      841 accgcgcacc catcgtagac atattgctcc tatggagcag actgacaagc cggagaaaat
      901 gtattcgagt ccattgcacg tgtgtgaatg ggtacgccaa tattttgagt tcgttgcgat
      961 aggacttgaa gaggaaaatg tgtgtgggat cagatgttgg gcttacgagc gatcacggag
     1021 gtggtggaat ggtacaacgc ccaatgggta gagtcctgtg agagtctgac atacacttga
     1081 atgtctttgg gtgagcataa gccggttttt aaatattcct gttcgagtac cagagccgat
     1141 tccgccatca ccctagccat tggtgaattg ccaggacaga gatgcatgag ggattgaacc
     1201 ttgacgatat ggaatcctgc ttcttgcagt ttgtgcggaa gcctgagggc ataggccgga
     1261 tctaatccga gattgatgaa catctggcaa atggctcggt ttaccattcc ttgtggggtc
     1321 tcttgcttcc ggtcctgcaa tgtcgccgag gtgaagtccg gttcctcgaa gagtgcccag
     1381 cctcctggct tgagaagaga gctcaatttc ctcagaattg ctccctcttc tttattatga
     1441 atcaggacat accggctatg aaggagatca aaggattgtt ctatggggac atcaagaatg
     1501 gagccttggt aaatttgaaa atgcggagaa tcatccttgt gaagatacgc gattttcttg
     1561 tcgacaccca ccgccagtcc tgtcggtccc acctgacttc ccatccaacg caagatggat
     1621 cctgcccctg cgcccacctc cagacaatgc caccctggtt gaattcctgt ttcctccaac
     1681 agtctgatgg tagtgggatc gttcgcggct tcaaccaatt gtagtcgttg aaattcccgc
     1741 tcttcagatt tgttgtcgaa aatatactga ttcatcaccc ttcctctgct gccgcgtgtt
     1801 tcatgatatc caaggccgca tggcatccgg ccagataccc tgtcgcccag gcccattgaa
     1861 agttaaatcc tccaatgcgg ccatcacagt ccaacatttc tccaatcata taaagcccag
     1921 gcacctgccg tgagcccatg gaatgcgcgg atatttcttc cagggggaca cccccggccg
     1981 tgacctcggc aaagttccat ccgcgtgatc cgatgacggg aagatcgaga ttggtgagtg
     2041 tccggaccag atcccggcga gcttctttgg acaaaaggtt aaccggagtg tcagaaagcc
     2101 caggttcttg gatgctggcc agcgtgccag ccacacgcgt gggtaatcgc tcggctaaca
     2161 gtgtgggaac aagttttcgc ccggggaggg aggcggcctg catgagccat ttctccacat
     2221 cctgattgga gaggtgaggg aagcaatgga gatgaaggtg gactgcttct ccttttgcgg
     2281 ctgcgcgaac ccaaacccgg ctggcatcca agaccaccgg cccactgatg ccgaaatgtg
     2341 tccacaacag gctgccggtg cgtcgatcga aggtttttcc cgcaatggta gtggtgagcg
     2401 ttacgtcatg agaaataccg gatagggtgg catggaaaaa cgcagggtca aggagcaaag
     2461 gaaccaaggc cggataggtg gcggtgacgg tatgccccaa ctgttgggcc aacctccaac
     2521 ctgatccatc ggatccggtt ttggggagag accggccacc ggtcgcgaga atgacccgtt
     2581 tgactcgaag cgttccctgg gaatgttgga tcgaaaactc ctccccttct ctggtcaggt
     2641 ctcgaacgag gtggtgagtc aaaagagcaa tgcccagcgc gtcgcaccgc ctgagaagcc
     2701 cattgagcac ggttcgggct ttgttgcttt cgggaaacag ttttccggtg gcttcagtct
     2761 ttaacgggat ccccaaagaa ctaaaccatc gaatggtggc ttgttcgtcg aagcgtcgaa
     2821 ggatccggtc aatcagcttt ctgttcccgt ggaagtcgga agcggtgaca cgttgattgg
     2881 tgacattgca gcgtccacct ccggatacca ggatcttggc cccgatggtt ttggcactgt
     2941 ccagaagggc gatagagaga ttcgggttcg tctccgccgc gaaaatgccg gctgccagtc
     3001 ctgctgctcc tgcgccgatg atggcgatgt cgatgtcgga agacataggc aatttctgga
     3061 acgagtgagg atctatctgg gttgggagtc cgacggttta cgaaacaaga cgacattctt
     3121 tt
//
LOCUS       DATIAL010000596         4304 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1180831, whole genome shotgun sequence.
ACCESSION   DATIAL010000596 DATIAL010000000
VERSION     DATIAL010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180831"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VLA60_17975"
     CDS             complement(<1..379)
                     /locus_tag="VLA60_17975"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HSF11304.1"
                     /translation="MFRKLKIFVVMGRYSSVNAITVGAILGMGVLCWPGGVLGVEAPT
                     VVVRGTIDEVIRLVSDEGLKKPDQEAHRRKLLEETIGQHFDFEEMAKRSLAAHWRNRS
                     ESEHQEFATLFQTLLSKTYAGKIE"
     gene            complement(571..1107)
                     /locus_tag="VLA60_17980"
     CDS             complement(571..1107)
                     /locus_tag="VLA60_17980"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HSF11305.1"
                     /translation="MAQLVVEKDKKKVAPVQKGELVRRGEYDRFLDRMDQMMAEVWGR
                     PFSGLWPSMPGMLRPSRALLGESLHVRVPAVDLYEGKEEVVLKAEVPGLSKEDLKIDL
                     TDSMLTISGEKKKEEEIKEEDYTYSERAYGSFSRSLQLPCAVKADKVKATFKDGILEV
                     KLPKTEDAKKRHVTVKIE"
     gene            1421..1612
                     /locus_tag="VLA60_17985"
     CDS             1421..1612
                     /locus_tag="VLA60_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11306.1"
                     /translation="MKQDNVERLKVKICGKTIVSVEPSRWSGEVKSLMKNQEFLSITL
                     SDGTVLEVGGLYIRDGSHT"
     gene            1702..2127
                     /locus_tag="VLA60_17990"
     CDS             1702..2127
                     /locus_tag="VLA60_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HSF11307.1"
                     /translation="MGLHAVATVGLRTIQQVATLHDFRFHHDQNGLAITQELLTSSLP
                     GAPVVDPNGHCVGFISQFDVLAVLEAGRDVSQLTAKEIMVPDPLAIPISTTLAEAVKI
                     MKDHHFLVLPVEENGVVIGCLTRQDLLRGWVGWGLEQKD"
     gene            2238..2618
                     /locus_tag="VLA60_17995"
     CDS             2238..2618
                     /locus_tag="VLA60_17995"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HSF11308.1"
                     /translation="MKKEQGPLSLVISAISCVCIMLGLGEVRAQQTTMISAGETIYRL
                     HCLRCHGKAGDGKGPDSSALIVPPTDFHSPESTAKSELDLRAIVIWGLVFSPMHGWWD
                     RLSSEEIREVIGYIRQLAPYQPRI"
     gene            2690..>4304
                     /locus_tag="VLA60_18000"
     CDS             2690..>4304
                     /locus_tag="VLA60_18000"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF11309.1"
                     /translation="MDELKIPFSQLAPTIDVDSLGFSDTTELPPLETPLGQARAIEAL
                     NFGLNMKSHGFNIYASGPIGTGKWAIIQKRVQHVASSMPDPPDWCYVYNFLEPSSPIC
                     LSFPASKGREFKQAMSFIIQSLQRDLPLAFESHKYLDARAKIIEERNEKKEALFKQIN
                     EEAATRGFGFKEDPVGFSLVPMRNGKPLREKDHDTLTATEKDTISEQGKILEGKIREF
                     QAQVHALDHEADHQLDEMDRQVVRAVMHNRFAVLRDHHQSLPDVLEYLQKVEEDIVAN
                     YKDFLPREGPQLALMGWDVRDRKPNLSRYEVNLLIEHAQHSGAPIIDEPHPTYANLIG
                     KIERKAHLGVVYTDFTEIRAGSCLRASGGFLMMNVLDLLRQPFSWEALKRVIKTRSVN
                     IEDPGEYFGFATTGLKPQPIPTDIKVILLGPPHIFALLQGYEQDFSKLFKVKADFEVD
                     IPRDTSQDQLYAGFIAQLCRDEGLPAFGADAVGEVIRHSLRLAESRDRLSLRLSLISD
                     VIREAAYWARQDGRTLVSNTHVKTALDKKR"
BASE COUNT         1124 a         1106 c         1002 g         1072 t
ORIGIN      
        1 tttcaatttt ccccgcatag gttttcgaaa gaagtgtttg aaacaatgtg gcaaattctt
       61 gatgttccga ctcacttcga tttctccaat gtgccgccag tgatcgtttg gccatctcct
      121 caaaatcaaa atgttgccca atggtttcct ccaacaattt ccgacgatgg gcctcttgat
      181 ccggcttctt gagaccttca tcgctgacca atcggatgac ctcatcgatg gtgcctctga
      241 ccaccacggt aggcgcttcc acccccagga caccacccgg ccaacacaac actcccatgc
      301 caagaatggc tcctacagta atcgcgttga cgctggaata tcttcccatc acaacgaaaa
      361 tcttcaattt cctgaacacc cggccccctc ctcagtaaat ggttttctga aaacccggtt
      421 ctctcagttt cgaagaggca tgcaaaaatg ttgaacaacg gccttaccgc tgccgatttc
      481 ttttgacgct ccctggggaa gacgtcgttc ttctccggga gcgcctgtca aacgcagatt
      541 cttaccgatc caaacacggg gagattgaca ctattcaatt tttacggtga catgccgttt
      601 tttggcatct tcggtcttgg gcagcttaac ttccagaatc ccatccttaa acgtggcctt
      661 cactttgtcc gctttcacgg cacaaggcaa ttgcaggctc ctggaaaagg acccgtaggc
      721 tcgttcggaa taggtatagt cttcttcctt aatctcctct tcttttttct tttctcctga
      781 gatggtcagc atggaatccg tcagatcaat cttaaggtct tctttggaga gacctggtac
      841 ttcggccttc agcaccacct cctcctttcc ttcatataag tcgacggcag gaactcgcac
      901 gtgcaatgat tcgcccagca acgctcgtga gggccgtaac ataccaggca tggatggcca
      961 taaccctgaa aaggggcgcc cccaaacttc cgccatcatt tgatccatac ggtcaagaaa
     1021 ccggtcatat tcccctcgac gaaccaattc acctttctga accggagcga ctttcttttt
     1081 gtccttttcc actactaatt gtgccatcac atacctcctt taaacatatg ataattgaac
     1141 ccagattttg caaatagctg aaatgcaact tatataccct tcttgacgat tggcgggagg
     1201 aaaaaactgt catgaccgcc agtattcatc cggcattcct caggtcttca ttgcggataa
     1261 ttcaccgcaa ataaccggcg ggatgacgaa agagagaggt tgtccaaaaa ggcacgaggc
     1321 ttgcttcaac tcatgtagga gcaaaggctc tgatttcatg gcgcttacgg aaacaatacc
     1381 atgcacacgt tatcaaccat tccaagaccg aggccaacac atgaaacaag ataacgtaga
     1441 acggctaaag gtaaaaattt gtggaaaaac aatcgtgtct gttgagccat ccagatggtc
     1501 cggggaagtc aaatctctca tgaagaatca agagttttta tccatcacct taagtgacgg
     1561 aacagttctg gaggttggag ggttgtatat tcgggatgga agtcatacct gatttcgtct
     1621 caaaccgttt aaattttcat tcactccatc cctgtgaaaa gacttttatg gggttatgag
     1681 tgaaagagag aggaggaaca catgggtctt catgcggtcg ccaccgtcgg cctcagaaca
     1741 atccaacaag tcgccacact ccatgatttc cgttttcacc acgatcagaa tggattggcc
     1801 ataacacaag aacttctcac atcttccttg ccaggcgcgc cggttgtcga tcctaacggg
     1861 cactgcgttg ggttcatcag ccaatttgat gtgctggccg tgctggaagc ggggcgggat
     1921 gtcagccaat taacggcaaa ggagatcatg gtgccggacc ctctcgctat tcctatttcc
     1981 accacattag cggaggctgt caaaatcatg aaagaccatc attttctggt acttccagtg
     2041 gaggaaaatg gtgtggtgat aggatgcctg acccggcagg atcttttacg gggatgggtg
     2101 ggttggggat tagaacaaaa agactaagtg aaatttgagc atatgaaaag tgttgcaatt
     2161 tgggtccatg gaaaagggaa ggggtgttgc aagatgcgac aaaatcgcat ttctatgaaa
     2221 cgtactgact tcacccgatg aaaaaagaac aaggccctct cagtctggtc atatccgcta
     2281 ttagttgtgt ctgcatcatg ttagggcttg gagaagtccg ggctcagcag accactatga
     2341 tctctgcggg agaaacaatt tatcgtctgc attgccttcg gtgtcatggg aaggcgggcg
     2401 acggaaaagg accggattca tccgcattaa ttgtgccccc aacagatttc cattctccgg
     2461 aatccaccgc caaaagcgag ttagaccttc gtgcgatcgt tatttggggc ttggtcttta
     2521 gccccatgca tggttggtgg gatcgattga gttcagagga gattcgggag gtaatcggct
     2581 atatccggca attagccccc tatcaaccca ggatctaaaa ttgcagtcga ggagaatcca
     2641 aggttcggaa aacaaccttc acacacagtc gcttggaaaa ttaacgcaca tggacgaatt
     2701 gaaaattcct ttttcacagt tagctccaac catcgacgtg gatagcttgg ggtttagcga
     2761 caccaccgaa ctgccgcccc tggaaacacc gctcggccaa gcccgggcca ttgaagcctt
     2821 gaatttcggg ctgaatatga aaagccacgg atttaatatt tatgcctcag ggccaattgg
     2881 tacgggtaaa tgggccatca ttcaaaagcg tgtccaacac gtggcctcat ccatgcctga
     2941 tcctcccgat tggtgttacg tctataattt cctggagccg tcttccccca tctgcctgtc
     3001 atttcctgcc tccaaaggac gggagtttaa acaggccatg agcttcatca ttcaaagtct
     3061 tcaaagagac cttcctctgg cgtttgaaag ccataaatat ttagatgcca gagcaaaaat
     3121 tatcgaagag cggaatgaaa aaaaagaggc gctcttcaaa caaatcaatg aagaagccgc
     3181 cacacgtgga tttggcttca aagaagatcc ggtaggattc agtctcgtgc cgatgcggaa
     3241 tgggaaaccg ttacgggaaa aagatcacga cacgttaacc gcgaccgaaa aagataccat
     3301 atccgaacag ggaaaaattc tggagggcaa aatccgtgaa ttccaagccc aggtccatgc
     3361 cttggatcat gaagccgacc atcaactcga tgaaatggat cgtcaggtcg tcagagcagt
     3421 catgcacaac cgctttgccg tccttcgaga ccatcatcaa tccctccctg atgtgttgga
     3481 atatctccaa aaagtcgaag aggacattgt cgccaactac aaggattttt tgccgcgcga
     3541 aggtcctcaa ttggcactca tgggttggga tgtacgcgat cgcaagccca atctcagccg
     3601 ctatgaagtg aacctcctta tcgaacatgc ccaacattcc ggggctccca tcatcgatga
     3661 gcctcatccc acttatgcca atttaatcgg gaaaattgag cgaaaggcgc atttgggcgt
     3721 ggtctatacc gacttcaccg agatcagagc cggttcctgc ctgcgcgcaa gcggcggttt
     3781 tttgatgatg aatgtgttag acctgctccg tcagcccttc tcctgggaag cgctcaagcg
     3841 ggtcataaaa acacgttcgg tcaacattga agatcccgga gagtattttg gttttgcgac
     3901 aaccggcctt aaacctcaac caattcccac tgacatcaaa gtcattttgt taggccctcc
     3961 ccacattttt gcactcctgc aagggtatga acaagatttc tcaaagctgt tcaaggtcaa
     4021 agccgacttt gaagtggata tccctcgtga taccagccag gatcagctct atgccggttt
     4081 catcgcgcaa ctctgtcgtg acgaaggact tcctgctttt ggagctgacg ctgtaggtga
     4141 agtgatccgg cacagccttc gtctagccga atcccgcgat cggctgtcat tgcgcctcag
     4201 cttgatcagt gatgtgattc gcgaagccgc ctattgggcc aggcaggatg gccggacatt
     4261 ggtgtcgaac acgcacgtga aaacggctct cgacaagaaa cgtc
//
LOCUS       DATIAL010000597         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_751288, whole genome shotgun sequence.
ACCESSION   DATIAL010000597 DATIAL010000000
VERSION     DATIAL010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751288"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VLA60_18005"
     CDS             complement(<1..447)
                     /locus_tag="VLA60_18005"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11310.1"
                     /translation="MEKNINQDLMALFVSMAGLLASRGENPYKVKAYRRAADSLKSLQ
                     EDVTELANRGELQTIPGIGKELSLKIREFLTTGRIRAYEELKTPLPDSVLEWVGLPGF
                     SEPIVHDLVFRLGITTWEDLEALAQSHLLQTLPGLGTRGEEILTAIQ"
     gene            694..903
                     /gene="thiS"
                     /locus_tag="VLA60_18010"
     CDS             694..903
                     /gene="thiS"
                     /locus_tag="VLA60_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HSF11311.1"
                     /translation="MKIVVNGEQRETEEPLTVAQLLETLHLRSEQVAVEINLKILDRG
                     DFLNWKLHDGDKIEILSFIGGGSPT"
     gene            923..1696
                     /locus_tag="VLA60_18015"
     CDS             923..1696
                     /locus_tag="VLA60_18015"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014959879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HSF11312.1"
                     /translation="MNQDPLIIAGRTFHSRLWVGTGKYQNFEETRKAIEASGADVVTV
                     AVRRVNITDNKSENLLDYLDPKKYTILPNTAGCYTVEDAVRYSRLARAAGVSDLVKLE
                     VIGDERTLFPDTAGLIEAAKILIAEGFVVLPYTNDDPIVAKKLVDIGCPAVMPLAAPI
                     GSGLGIRNPYNLKIIMETVNVPVIVDAGVGTASDAALAMEYGADAVLMNTAIAGAKDP
                     LMMATAMRYAVDAGRLAYRAGRIPRKLYATASSPIEGML"
     gene            1709..2335
                     /gene="thiE"
                     /locus_tag="VLA60_18020"
     CDS             1709..2335
                     /gene="thiE"
                     /locus_tag="VLA60_18020"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HSF11313.1"
                     /translation="MSSRILPRLYLLTDRHQTLHRPLPSVITEAVDAGVRMVQIREKD
                     LTTRELTALCQQLCPLIKHHQGTILLNDRIDLVLALGADGVHLRTDSLPVSIARRLLG
                     TGHLIGVSTHSVEEARVAEAEGADFIVLGPIFDTPSKRAYGPPLGIQILQEAKRFLHL
                     PIYAIGGITPIRIPDVLSAGAYGVAVISSILQSPSIPDTTRELLARLS"
BASE COUNT          688 a          683 c          622 g          675 t
ORIGIN      
        1 ttgaatcgcc gttaagattt cctcacccct ggtccctaga cctggaagag tttgcagtaa
       61 atgcgattgg gccaaggctt ccagatcttc ccaagttgtg atacccaatc gaaaaacaag
      121 atcgtgaaca atcggttctg aaaatcctgg caggcccacc cattccagga ccgaatcagg
      181 cagaggagtt tttaattctt catacgcacg gatacgaccc gtcgtgagaa attcccgaat
      241 ttttagggat aattccttcc caatgccggg aatcgtttgg agttcgccac gattcgccag
      301 ctctgtcaca tcctcttgga ggctcttcag tgaatcggca gcccggcgat aggccttgac
      361 cttgtaggga ttttcacctc tggatgccaa caaacctgcc atcgacacga acaaagccat
      421 gagatcctgg ttgatgtttt tctccatgac gatcgcctca gtgggacaag cttgctttcc
      481 tcacacctca aggatccact gtgtgcctta atcattttat gtcagatgta atcgataaaa
      541 ccaggtcata atgatacgcc ttcattggct caccagaacc attgccatcc ttcttcaccc
      601 cacatcattt cattgacaag aatttctcaa gtcccgtagg atgaacttct gcatggccgg
      661 aatacactgg acattgcgtt cttatctata cgcatgaaaa tagtcgtgaa tggagagcaa
      721 cgcgagaccg aggaaccttt gacggtcgca cagcttctag aaacgcttca cctccgttcc
      781 gaacaagtcg cagtagaaat caaccttaaa attttggatc gcggagattt cctcaactgg
      841 aaactccacg atggggacaa gatcgaaatt ttaagcttta ttggaggcgg ctctcccacc
      901 tgaggagatt gaaggggaag agatgaatca ggatccgtta atcattgccg gtcgaacatt
      961 ccactctcga ctttgggttg gaacaggaaa atatcaaaac tttgaggaaa cccgaaaagc
     1021 gattgaagct tccggggctg atgtggtgac ggttgcagtt cgccgcgtca atattaccga
     1081 taacaaatca gaaaatttac tggactacct cgatccgaag aaatacacca ttttacccaa
     1141 cacggccgga tgttataccg tcgaagatgc cgttcgttat tctcgactgg cccgcgctgc
     1201 gggagtttca gatttggtga aattggaagt cattggagat gaacgcacac tatttcctga
     1261 tacggcagga ctcattgaag ccgcaaaaat tttgattgcc gagggttttg tcgtattgcc
     1321 atatacgaat gacgacccca ttgtcgccaa aaagttagtc gatattggct gtcctgccgt
     1381 catgccactc gccgcaccga tcggttctgg gcttggaatt cgcaatccgt ataatctcaa
     1441 aatcatcatg gaaacggtta atgtaccggt cattgttgac gccggagttg gaaccgcttc
     1501 tgacgcagcc ctggccatgg aatacggagc tgatgccgta ttaatgaaca cggctattgc
     1561 cggagccaag gatccgttga tgatggccac ggccatgcgg tatgccgttg acgccggacg
     1621 attggcttac agggccggaa gaattcccag gaagttatat gcgactgcca gcagccctat
     1681 cgagggaatg ttgtaaccca tattcggaat gtcctcgcgc attcttccca gactctacct
     1741 tctgaccgac cgccatcaaa cccttcatcg tcccctccct tccgtgataa ccgaagcggt
     1801 agacgcaggg gtccggatgg tacaaatccg ggaaaaagat cttacgaccc gagaattgac
     1861 tgccctctgt cagcagctct gccctctcat caaacaccac cagggaacca tcttattgaa
     1921 tgatcgtatc gacttggtcc ttgccttggg agccgatggc gtacatcttc ggacagacag
     1981 tcttcccgtt tccattgccc gtcgcctctt aggaacagga cacctcatcg gcgtctcaac
     2041 gcattccgtt gaagaagccc gagtggcgga agcggaaggt gccgacttca tcgtgctggg
     2101 tcccattttt gatacgcctt caaagcgagc gtatggacca cctttaggaa tacaaattct
     2161 tcaggaagcc aaacgattcc tccacttgcc catctatgcc attggaggaa tcaccccgat
     2221 cagaattcct gacgtcttat cagcaggggc ttacggtgtg gccgtcattt cctctattct
     2281 tcaatcgccc tcaattccag acaccacccg tgaactgttg gcacgattgt cctgaattgt
     2341 caacttattt tagccgtcac aatgcgggtg tttggttgac cctaattttt tttgggtggt
     2401 aagatctttt cagagaagga acgtgcggat ttcccattta ccacttaata tactgacagg
     2461 tcgggagatt tattgagagc gagatgtgcc attcggtttc gttgaaacct tgaatcaggt
     2521 aggatctaac tatgggtatc cgaaaaacag acgatcatat ccgtgaaatt gaaaaattac
     2581 gaaccgagat tgagtccatg gaactcgaaa tgcggcagct gcatgaatct cgacagaagc
     2641 tgcacctttc tcaacagaaa aacgagca
//
LOCUS       DATIAL010000598         2400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_214775, whole genome shotgun sequence.
ACCESSION   DATIAL010000598 DATIAL010000000
VERSION     DATIAL010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214775"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..964)
                     /locus_tag="VLA60_18025"
     CDS             complement(254..964)
                     /locus_tag="VLA60_18025"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HSF11314.1"
                     /translation="MMNWKLLVGMLGVVLWVGGCAETYHVKGDVEQTGFLRDVYPLMK
                     PGKEGEANLIYRNPDARWKTYNKIMLEPVTVWLGKDAVAKDTGVIPPDDVRDLANMFY
                     QKLSDQLSKDYTLVHESGPDVMRISSALTDVENGMPVLDAVSTLHPVSHILSEGKKLA
                     TGTHGFVGGASVEFRLADSQTGKLLAAGIDREAGGKTLTKGFGYWTDVENIMDYWAAK
                     LRWRLCTQRKGTDCQSPD"
     gene            complement(1851..>2400)
                     /gene="pgi"
                     /locus_tag="VLA60_18030"
     CDS             complement(1851..>2400)
                     /gene="pgi"
                     /locus_tag="VLA60_18030"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960735.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HSF11315.1"
                     /translation="DGTVVDYQTGPIIWGQPGTNGQHAYYQLIHQGTRLIPCDFIGFY
                     RSLNPSGPHHDLLMANFFAQTEALAFGKTAKEVEAEGVPSSLVPHRTFVGNHPTNTIL
                     AEELTPAMLGKLIALYEHKVFVQGTIWNLNSFDQWGVELGKVLAKRIAPELTAKKEPD
                     LGHDSSTNTLIRRYRQNRRSQS"
BASE COUNT          598 a          629 c          550 g          623 t
ORIGIN      
        1 tccatgttga gtatcgatga aacagccgaa ggatatgcgg tctagtgggg gtggtgagcc
       61 gtacgatttc cttagcacaa tgcaaataag taagatttgt tatttcccgt gtatgactag
      121 attttgtttg tcagcaatca gagcatatgg ggccctgcat gcagggcccc acaccggccg
      181 ggtaggcacc aagcctttcc gggcgaaaag gagcagtcct tgcgccagcc tatccctttc
      241 cttacaacac caatcagtct ggtgactgac agtcggtgcc ttttcgctgg gtgcacagac
      301 gccatcgaag cttggcagcc caatagtcca tgatattctc gacgtcagtc caatacccaa
      361 agccctttgt aagggtcttg cctcctgcct ctcggtcaat tccggcagcc agcaatttac
      421 ctgtttggga atctgcaagc ctgaactcga cgctggctcc gccaacaaaa ccgtgggtgc
      481 cggtggcgag ttttttgcct tcagaaagaa tatgcgaaac ggggtgaagg gtagaaaccg
      541 catccaacac gggcatacca ttttcgacat ccgtaagagc ggatgagatc cgcatcacgt
      601 caggaccgga ctcgtgcacg agggtataat ccttggatag ctgatcggaa agcttttgat
      661 aaaacatatt ggcgaggtct cgaacatcgt caggtgggat cactccagtg tcttttgcca
      721 cagcatcttt tcctaaccag acagtcaccg gctcaagcat gatcttattg taggtcttcc
      781 atctggcgtc aggattccta tagataagat tggcttcccc ttctttcccg ggtttcatta
      841 aaggatacac atcccggaga aacccggttt gctccacatc acctttaaca tgataggttt
      901 ctgcacatcc accaacccac aggaccaccc ccagcattcc gaccagtaac ttccagttca
      961 tcatcagacc tcctgttcac ccaccatagc ctttaaatgg tgaagaaaaa ttgtgtttac
     1021 ggaattcatg gggcgacagc ctcagacaaa caatccacaa cgtctgtcct gagtcacgcc
     1081 aaaggcctcc catgaacacc gtattgcgcc ataattagat cacgtcagag atgttattta
     1141 tcataaatct cacaattcta acacacagct aggttgaatc ccgagcaact tctgcccagg
     1201 gcccttgaag cagataccac tatagccaaa cctggttttg aaatcctttc accgcaccgt
     1261 cggctaactg tatctctttg gtttcaagag cttgacctat gtgccctttc gcgtcgctca
     1321 ttttttcctt tcattatcca catgaaccca caaacctatt tccgcctccg aaagtcggaa
     1381 cggattaatt aggtgagagg agtttgatca aaagaagcca gcttggcaag atgcgttcaa
     1441 tcaaggctga aagcgaattg ggtcaatttt agaaatgagc caggtcaatc aacctattca
     1501 agtttccatc tctttcattc caaacatttc gccctcaaca tctcccctac accactttca
     1561 gacccttttt gtctcctggt gacgatgact tcatggtccg ttcctatttg gctaatggta
     1621 tctcaaccaa aatgcatatt ttcccgatta cggagagtaa tcgccgtttg catgtctgcc
     1681 tctgtggtgg gggcttgatc tactgcggta tcagaccgca tattgccagc atcaggcaga
     1741 ctaaatttcc cgctctgact tggctaattt cattgattct cctctttaaa gaccgtaaaa
     1801 atggaggtgt aatcatcgga aattttcatg acccaacaag tatagaaaaa tcagctttgg
     1861 gatcgacggt tctgccgata gcgtcgaatg agggtattcg tggagctgtc atggcccaga
     1921 tccggttcct tctttgctgt gagttctgga gcaatcctct ttgcaagcac ctttcccaat
     1981 tccactcccc attgatcgaa ggaattcaga ttccaaatgg tcccctgcac gaacacttta
     2041 tgctcataca gggcgatgag ttttcccaac atggccggtg tgagttcttc agccagaata
     2101 gtgttggtgg gatgattgcc gacaaacgtt cgatggggaa ccaaggatga aggaacaccc
     2161 tcagcctcaa cctccttggc tgtcttgccg aaagcgagag cctcagtctg ggcgaaaaag
     2221 ttggccatga gcaggtcgtg gtgcggcccg gagggattca aggagcggta gaagccgata
     2281 aagtcacagg gtatcagcct ggttccttgg tgaatcaatt gataataggc atgctgaccg
     2341 ttggtgccag gctggcccca gatgattggg cctgtctgat aatccacaac ggtcccgtcc
//
LOCUS       DATIAL010000599         4942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1182325, whole genome shotgun sequence.
ACCESSION   DATIAL010000599 DATIAL010000000
VERSION     DATIAL010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182325"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1282
                     /locus_tag="VLA60_18035"
     CDS             179..1282
                     /locus_tag="VLA60_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HSF11316.1"
                     /translation="MISQQQCQNATILIVDDQMTNIMFLESILQNAGYTHVHSIQDPT
                     QVVPRFKELNPDLICLDIRMPQINGFQIMGQLKIIHKQAFLPILVITSEDDRDTRLRA
                     LESGAKDFLIKPFDKVEVLMRIRNLLEASLLNKTVSQQKEILEETVRIRTRELKETQF
                     DVVHRLARAAEHRDNETGSHIIRMSHFAVVLGRACGMSEDECDVLFHATPMHDVGKLG
                     IPDRILLKPGKLDQDEFEIMKQHTVIGAKLLANSHSPVLQMGEVIALTHHEKWDGSGY
                     PNRLSGEDIPLVGRICAIADVFDALSSKRCYKDPWPLEKTLHEINSLSRKQFDPHLVG
                     VFNELLPLIIDIQRTHTDVEIPKFELLETTCHT"
     gene            2008..>4942
                     /locus_tag="VLA60_18040"
     CDS             2008..>4942
                     /locus_tag="VLA60_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HSF11317.1"
                     /translation="MTFAPTGHRVIWFVWFLVFLGVLLGIVTLGLTRYTLFEVQTNKI
                     VAMEAGSLQGEMGNALNHLGVQAVKQIENTLENPIENTLNPDQFDAYLHALNERLDQY
                     PTGDMHEILSQMLQTGQRLQTLGGQVRKWVQRSRPILKDLQSQQTIKIARERLRTLGI
                     HIQTWEGNTRLAQTMAYRKRKQDPSSEAPVIPDDLLAHQIRQAALGVSIIQTELGNLS
                     VLLETLYGERNPDRLVDLKDNQIKQNLTRLSIGMGMLVGDSLIPNDTVQKFLQDLGSL
                     IFGHGYVIDEVHQTVRLGDGGLYRFKQSSLGLEEERAKFYLSLETIKEQIGEAQVSFA
                     KLASQQTQEVTNKFENSFAQSWRFMLTLSLICVAGFLSTAWMISRWIHKQLRLLETAK
                     NQAESAVQAKSQFLATMSHEIRTPMNGVIGMTGLLLETELTSQQRQLANTVRSSGETL
                     LTLINDILDFSKIEAGKLEFEIIDFDLRTTLEETLDLLAGPAGTKQLELVGLVSATVP
                     TALQGDPGRLRQILMNLVGNAVKFTANGEIVVQMQVLGETTDTVTIRVEVRDTGIGIS
                     PEAQAKLFQPFSQADSSTTRQFGGTGLGLAISKQLVEQMGGTLGVESLPGKGSTFWFT
                     ACLKKQPAPVQPPNPGNIRLMGLTVCGVDNHPTNRHLLAQYFQDWCMNGFLAATPTEA
                     LTLLRQAAHQGNPVDLAIVDMEMPEMDGLRLAHAIKADPTIAAVRLVLLTSLGRHGDA
                     KAAQDAGFTGYLTKPIRKSQLQACLETVMGYGPRNPATVSRPFITSHQPQDWRRNQAA
                     RILVADDHHVNQQLAVMLVERLGHRADVVANGQEAIEAVSRIAYDVILMDCHMPELDG
                     YEATRAIRQSESQTRHTPIIAMTANAMTGDREKCLSAGMDEHLSKPIRPEELARVLAK
                     WLPQQTKETSPQDKAPPPLPPSLGHSTLNVRSLIELENLGGREFLQAMIQKFVEDALE
                     CVTLIE"
BASE COUNT         1312 a         1286 c         1173 g         1171 t
ORIGIN      
        1 tctgtcgtca agtttatggg agacgttccg aaaatgagta aacgtacagg aagaacaacc
       61 agttctcttt tgttgaacca tcttctgact caacactgta aaaaccatct tcatcagagt
      121 tcaatatgac atacttcatc atccaatcgg accaaacaat cactccactc cttttcccat
      181 gatttctcaa caacaatgcc aaaacgccac gattctcatc gtggatgatc aaatgaccaa
      241 tatcatgttc ttagagagca ttttacaaaa tgccggatat actcatgtcc attcaattca
      301 agaccccact caggttgtgc ctcgattcaa agaactgaat ccagatctaa tttgtctgga
      361 tattcgcatg cctcagatca atggctttca gatcatggga caactcaaaa tcattcacaa
      421 gcaagccttc ctgcccattt tggtaataac gtcggaagac gaccgggaca ctcggctacg
      481 cgcattagag tcaggcgcca aggacttcct aattaagccc tttgataaag tggaagtctt
      541 gatgcgaata cgaaatttat tggaagccag tcttctcaac aaaacggttt ctcaacaaaa
      601 ggaaatcctt gaagaaaccg tcaggattcg tacaagagaa cttaaagaga cccagtttga
      661 tgtcgtgcac cgcctcgctc gcgcagccga acatcgcgac aatgaaaccg gcagtcacat
      721 cattcgtatg agccattttg cggtggtctt aggacgagcc tgtggtatgt ccgaagatga
      781 atgcgatgtg ctttttcacg ctacaccgat gcatgatgtg gggaaattag ggattccaga
      841 ccggattctg ttaaagcccg gaaaattgga tcaagatgaa tttgaaatca tgaaacagca
      901 taccgtcatc ggagccaagc tgcttgccaa cagccactct cccgtcctgc aaatggggga
      961 agtgatcgcc ctcactcacc acgaaaaatg ggatggttcc ggatatccga atcgtctgtc
     1021 cggggaagac attcctttgg tcggtcgaat ttgtgctatt gcggatgttt tcgatgctct
     1081 ctcatctaaa cggtgttata aagatccctg gccccttgag aaaacccttc acgaaatcaa
     1141 ttcactttct agaaaacaat tcgatcccca cctggttggc gtatttaacg agcttctgcc
     1201 cctgatcatc gacatccaac gaactcacac ggatgtggag ataccaaagt tcgagctcct
     1261 ggaaaccacc tgtcacacct aagtccctgg cttcaaccag gtattctccc cctcatcctt
     1321 tctgttccgt gcctccgtct ttgaccatgg gttttatcat gttagtgagt gaggacgatc
     1381 cgcaattcgt ctccagccgc atcttttgca ccttcaaagg cccgaatcga cgatgttctg
     1441 aaatcaggca ggaggaaggt gaaagaatgg cgatacatgt taagtttcag gtccgagtac
     1501 cagctctgct tcgccatttc cttttcaact tttccaaaca atacagatta ctttttaaaa
     1561 atccgataac ctcagatggg gcatgtgcgt aataccttag gcctgtggaa atgtccgtaa
     1621 gccgtaagga gtgtggcatg tagagtgact ctcattgaca catttatgct gtaagaaatt
     1681 agggtcctta tcaagccgga gttgaggcaa cgagtatgta tgaacgttca atcttcatct
     1741 ccgaacattt caatccattc gttactcggc attgttatca ggaacctgca aaattctctg
     1801 caaatttatc tttgataatt cccactttct gaaggactgg acattggaac atttgggaac
     1861 tttgacttca aactggagta ttcaccttga ttggacaccc acctccaggg gaaccaccag
     1921 gaatggggca attagttaag ggtattgagt tgatttcata tggttgggaa aaatgactca
     1981 caattatgca gagaaactaa attcctaatg acctttgctc cgacaggaca ccgtgtcatt
     2041 tggttcgtgt ggtttctcgt attccttggt gtgctccttg gtattgtcac cctaggcctg
     2101 acgagatata ccctctttga agtacaaaca aacaaaatag ttgcgatgga ggcagggtcg
     2161 ttacaagggg aaatgggtaa cgcactgaac catctcggtg tgcaagcagt gaaacagata
     2221 gaaaataccc tcgaaaatcc tattgaaaac acacttaatc ccgatcaatt tgatgcttac
     2281 ctacatgcat tgaatgagcg attagatcaa tatcccacgg gagacatgca cgaaattttg
     2341 agccaaatgt tacaaactgg tcagcgcctg cagaccttgg gtggccaagt gagaaagtgg
     2401 gtccaacgtt cccggccaat cttgaaagac ctgcagtcac aacagaccat aaaaatagct
     2461 cgggaacggt tgagaacatt ggggattcat attcaaacct gggaagggaa cacacgcctg
     2521 gcgcaaacta tggcctaccg gaaaagaaaa caggaccctt cttcggaagc gccggtgatt
     2581 cccgatgact tgctcgctca tcagattcgc caagccgccc ttggcgtgtc gattattcag
     2641 acagaattgg gaaatctgtc cgtgttattg gaaaccctgt atggcgaacg caatcccgat
     2701 cggctcgtcg atttaaaaga taaccagata aaacaaaatt tgactcgact ctcgatagga
     2761 atgggcatgc tggtaggaga ctcgctcatt ccaaacgaca cggttcaaaa gtttcttcag
     2821 gacttaggat ccctcatatt cggtcatggg tatgtaatag acgaggttca tcaaactgtt
     2881 cgcctaggcg atggaggact atatcgtttc aaacagagct ctctaggtct agaggaggaa
     2941 cgtgccaaat tctatctaag cttggaaacc ataaaagaac agattggtga ggctcaggtg
     3001 tctttcgcca aactggcatc tcaacaaaca caagaagtga caaacaaatt cgaaaactcg
     3061 ttcgcacaaa gctggaggtt catgttgacc ctcagtctga tatgcgtagc cgggtttttg
     3121 tccactgcat ggatgatttc ccgttggatt cacaaacaat tgaggctgct ggaaaccgcc
     3181 aaaaaccaag ccgagtccgc cgtgcaagcc aaatcgcaat tcttggccac catgagtcat
     3241 gagattcgca ccccgatgaa tggcgtgatt ggcatgaccg gccttctcct ggaaaccgag
     3301 ctgacttctc agcaacgcca gttggccaac accgtgcggt cctccgggga aacgctctta
     3361 acccttatta atgatattct cgacttctca aaaatcgaag ccgggaagtt agagttcgag
     3421 ataattgatt tcgatctgcg caccacctta gaggagacgc tcgacttgct ggccggtccc
     3481 gctggcacga aacagttaga actcgtggga cttgtctctg cgacagtgcc gaccgctctg
     3541 caaggagacc ccggacgcct gcgtcagata ctcatgaatc tcgtagggaa tgccgtcaaa
     3601 tttaccgcca acggtgagat cgtcgtccag atgcaggtcc tgggtgagac gacagacacc
     3661 gtgaccattc gcgtggaggt ccgggatacc ggcatcggca tatctccgga ggcccaagcc
     3721 aaactcttcc aacctttttc gcaggccgat agttcgacca cccggcagtt tggcggcacc
     3781 ggccttggcc tggccatttc caagcaactt gtggaacaaa tggggggaac tctgggggtg
     3841 gagagcctcc cgggaaaggg cagtaccttt tggtttaccg cctgtttaaa aaaacaaccc
     3901 gcccccgtcc agccccccaa ccccggcaac atccggctca tgggcctcac cgtctgcggg
     3961 gtcgacaatc accccaccaa tcgccattta ttagcccagt attttcagga ttggtgcatg
     4021 aatggctttc tggctgccac ccccacggaa gccctgaccc tcctacggca ggcagcccac
     4081 caaggcaacc ctgtagacct cgccatcgta gatatggaaa tgccggagat ggatgggctg
     4141 cgattagccc acgcgattaa agccgacccg acgattgcag ccgttcgtct ggtgctgctc
     4201 acctcattgg gcaggcatgg agatgccaag gccgctcaag acgccgggtt taccggctat
     4261 ttgaccaagc cgattcggaa aagtcagtta caggcttgcc tggaaacggt gatgggctat
     4321 ggaccccgga accccgccac cgtctcgcgc cccttcatca cgtcgcatca accgcaggac
     4381 tggcgtcgta atcaggcagc acgcattctg gtggccgacg atcaccacgt gaatcagcaa
     4441 ttggcagtca tgctggtgga acggctcggg caccgggccg acgtggtggc caatgggcag
     4501 gaagcgatcg aggccgtttc ccggattgcc tatgatgtca ttctgatgga ttgtcatatg
     4561 cccgagctgg atgggtacga agccaccagg gccattcgcc aatcagaaag ccagacgcgc
     4621 catacaccca ttattgccat gaccgccaat gccatgacgg gggatcggga aaaatgtctt
     4681 tccgccggca tggatgaaca tctcagcaag cccattcgcc cggaagagct ggctcgtgtg
     4741 ttggccaaat ggcttcctca gcagaccaag gaaacctccc cacaggacaa agcccctcca
     4801 ccccttcccc cgtcattagg gcattccacc ctcaatgtcc ggagcttgat agaactggaa
     4861 aacctagggg gcagggagtt tctccaagcc atgattcaga agtttgtaga agatgccctg
     4921 gagtgcgtga ccctcattga ac
//
LOCUS       DATIAL010000600        11699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Nitrospirales bacterium isolate SMAG_U16263
            k141_1074807, whole genome shotgun sequence.
ACCESSION   DATIAL010000600 DATIAL010000000
VERSION     DATIAL010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935758
            Sequence Read Archive: SRR25158334
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Nitrospirales bacterium (soil metagenome)
  ORGANISM  Nitrospirales bacterium
            Bacteria; Nitrospirota; Nitrospiria; Nitrospirales.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2024 04:34:36
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,622
            CDSs (total)                      :: 3,588
            Genes (coding)                    :: 3,573
            CDSs (with protein)               :: 3,573
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 2 of 15
            Pseudo Genes (incomplete)         :: 10 of 15
            Pseudo Genes (internal stop)      :: 4 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Nitrospirales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074807"
                     /isolate="SMAG_U16263"
                     /isolation_source="soil"
                     /db_xref="taxon:2358460"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /gene="purB"
                     /locus_tag="VLA60_18045"
     CDS             <1..1278
                     /gene="purB"
                     /locus_tag="VLA60_18045"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HSF11318.1"
                     /translation="EAMEQMGHVPYGVAARIRKKVTINPVRIAAIEQVTKHDVIAFLE
                     SVSEQAGKDGRFLHVGLTSSDIVDTSLSLQLAEAAQLIREDVGELLNVLRDLAFAHQN
                     TLMVGRSHGIHGEPISFAWKVAIWYQEFQRQELRLKAAIADIAVGKLSGAMGTFAHLS
                     PSIEKTVCQRLGLKPAPISNQVLQRDRHAAFLSALALMAASIEKVATEIRHLQRTEVL
                     EAEEYFEPGQKGSSAMPHKRNPISSENLCGLARVVRSNSLAALENVALWHERDISHSS
                     AERIILPDSTILIDYMLVRLTNMLSKLVVYPERMMATLNQTGGLIYSQRLLLALVDKG
                     AIRKDAYEHVQQHAMAAWRGEGSFQALIEQDPFIAKHLSTKEIATCFNPKTYLRHQQE
                     IFTRVFGRQVRRKSSTPRVTPSKKTLKRGKYID"
     gene            1271..1975
                     /gene="purC"
                     /locus_tag="VLA60_18050"
     CDS             1271..1975
                     /gene="purC"
                     /locus_tag="VLA60_18050"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HSF11319.1"
                     /translation="MTKGTMLYEGKAKKIFLTEKDDELIQYFKDDATAFNAEKRGTIL
                     EKGIVNNAISAKLFQHLADAGISSHFIQQINDREMLTRRVKIIPVEVVVRNRATGSIV
                     KRLGLEEGLIIDPPLVEFFYKNDSLGDPLISEDHIRLLKLATPGQIEELRQQALKINE
                     VLKPFFQKKGMLLVDFKLEFGLWNGQIILADEISPDTCRFWDIQTGERMDKDRFRKDL
                     GRIEETYQEVLKRVCG"
     gene            2091..2336
                     /gene="purS"
                     /locus_tag="VLA60_18055"
     CDS             2091..2336
                     /gene="purS"
                     /locus_tag="VLA60_18055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HSF11320.1"
                     /translation="MKAKIYITLKNGILDPQGRAIQQSLQTLGFGSVGEVRIGKFIEV
                     DLCETDATSGEQTIKAMCEKLLANTVIEEYQYEILEA"
     gene            2428..3141
                     /gene="purQ"
                     /locus_tag="VLA60_18060"
     CDS             2428..3141
                     /gene="purQ"
                     /locus_tag="VLA60_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014961275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HSF11321.1"
                     /translation="MNIGIIVFPGSNCDRDCAHVLSDIMGQTAEFIWHQERSVKGMDA
                     IILPGGFSYGDYLRTGAIARFSPIMEAVVEFAGRGGIVLGICNGFQMLLELGILPGAM
                     LRNRDLSFICEDHYIRVENANTPFTNHCKPGQILKLPIAHAEGNYYTNPVTLSALQAN
                     AQIVFRYCDREGKVTSQTNPNGSLDNIAGIRNAAGNVLGLMPHPERAAEHLLNNEDGL
                     YIFRSMVSALEKHKTPQPV"
     gene            3211..3417
                     /locus_tag="VLA60_18065"
     CDS             3211..3417
                     /locus_tag="VLA60_18065"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HSF11322.1"
                     /translation="MTPGICHGKPCVAGTAVDVATVVGTLGIGKSFHEVQEALNLTYE
                     QVLTALRYASYVTDHLPLRLPSGR"
     gene            3627..5900
                     /gene="purL"
                     /locus_tag="VLA60_18070"
     CDS             3627..5900
                     /gene="purL"
                     /locus_tag="VLA60_18070"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HSF11323.1"
                     /translation="MTQESKVLPQVPLTSKIIEQHGLSEDEYQQILKHLGREPNLTEL
                     GIFSVMWSEHCSYKSSRIHLKRFPTEGERVVQGPGENAGAVDIGEGLAAVFKMESHNH
                     PSYIEPFQGAATGVGGILRDIFTMGARPIALLNSLRFGELDLPKNQHILKGVVSGISS
                     YGNCMGVPTVGGEITFNDIYSKNPLVNVFCLGIVKKDRLLRGLAKGAGNQVLYIGAKT
                     GRDGIHGATMASDSFDDASEKKRPNVQVGDPFLEKLLLEACLEFLEQDLLVGIQDMGA
                     AGLTSSSCEMASRAGTGIDMDLAKVPRREQHMTPYEILLSESQERMLLVAQPGKEESV
                     LAICRKWGIDAAVVGQVTDDGFLRIREGNQIVAEIPAHALAEEGPRYERPAAPPPYQE
                     FLQSLNIDTLPDVKNPAEVLLKLLASPTIASKRWVFRQYDHMVGTNTVVCPGSDSAVV
                     RIKGTTKALAMTTDGNSRYCLLNPYLGGGLAVAEAARNLVCAGAQPIGVTDCLNFGNP
                     ERPDIMWQFIMAVEGIADACRTFQIPVVSGNVSFYNETNGLSIYPTPILGMVGLIDRP
                     EDITTQWFKQSGDHIFLLGETKEDLGGTEYLKVIHSREQGNPPWLDLDREKALYSFLL
                     TLIQKGLVQSAHDCSEGGLLVTLAECCLTHPSALFGATVILNQDRLRLDALLFGESPS
                     RVVVSVKSEHVKQLMELVQGTDVPCTELGQVTQGNMDINVRGAQDRPVCEISLPLSEM
                     EDQWHHALARQLGAEAS"
     gene            5995..7431
                     /gene="purF"
                     /locus_tag="VLA60_18075"
     CDS             5995..7431
                     /gene="purF"
                     /locus_tag="VLA60_18075"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HSF11324.1"
                     /translation="MVQSMPSPDGFHEECAVFGIAGSKEAANLTYLGLYALQHRGQEG
                     SGIVSSDGEQFFQKKGLGLVADIYSKKTLRELPGTKAIGHNRYSTAGSGQLQNVQPLS
                     VNFAFGNLALAHNGNLVNAGVLRGELEAYGAIFQSDSDSEVIIHLIAHSKGDTIVNRV
                     IDALSLVRGAFSLVLLTDDQLIAARDPFGFRPLCLGRYKSSWVVASETCAFDLIDAKF
                     VREVEPGEIIVIQGTELTSYHPFLERSRAQCVFEYVYFARPDSKIFGPNAVYPVRKAF
                     GRQLAKECPADADMVIPVPDSGVPAALGYAEGMGLPFEIGLTRNHYVGRTFIEPQQAI
                     RHFGVKLKLNPVPDVLEGKRIVVVDDSLVRGTTSRKIVKMLRQAGAREIHLRISSPPV
                     IAPCYYGIDTPTQKELIGSNLNIEEIRRYVTADSLGYLSLEGMLAMAPGFSHHYCNAC
                     FTDNYPIMLTKAEQLQLGLFEPDQLSRS"
     gene            7545..8099
                     /locus_tag="VLA60_18080"
     CDS             7545..8099
                     /locus_tag="VLA60_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HSF11325.1"
                     /translation="MKRNILQLLIVVILILSTGFIAQAGSFFKVGESAPNFSLTGLDG
                     RPLSLDQFKGKVVVLGLFHICEPCLIQSTNLQRVHEATKDKPVAVIGVNSSGDSLSDV
                     REFLQGFPLKITYPYLLDPDKKTDKLYGGGKFIPNVYVIDQNGLIRWQRVGNFFTDGT
                     LAGHEAILQEVEKLLEAPPTPSTM"
     gene            8147..8386
                     /locus_tag="VLA60_18085"
     CDS             8147..8386
                     /locus_tag="VLA60_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11326.1"
                     /translation="MDELEDGKQKFLEIVKSLDAAVEVVIPTTPSRSLLLISLTKGPN
                     RKFITVHEDDMLDLPEDLGVQAKTTALLQETLETL"
     gene            8383..8982
                     /locus_tag="VLA60_18090"
     CDS             8383..8982
                     /locus_tag="VLA60_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11327.1"
                     /translation="MKTILPNISEWSWFSEEKQLNFNGHLLAVGEHRILVDPPPMNAS
                     DRAITQRGGALDYIILTNRDHEREAANFRKIFNCQVMAPELDAKEMTLPIDKTFKDGE
                     LLPGGIWVVQLSHQKSPGESALFLQQGQGVLIVGDGIIGDPPGSLRLLPSEKYIDIVQ
                     ARDSLRRLLKYTFDSLLVGDGTSLLTGAKPALEQLLANE"
     gene            9046..9849
                     /locus_tag="VLA60_18095"
     CDS             9046..9849
                     /locus_tag="VLA60_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HSF11328.1"
                     /translation="MAWSQSANLNSLGNRHFSKGFYTEAFQCYAQALEVDRQNGDQRS
                     LATTLGNLGNICAVSGRREQARAYYSEVLELQKILGDERGISTTLANLGNLSADAGEW
                     DRARAYYLEALDLMNHLSDYAGKAVLLSDLGLVARETGKEEDALAFYQESLILMRRVG
                     NEAGQSDVFRMMARLYLSQRRFEEAQSCTLTSLDVARRLKDELRMGGAWYVLASCHEA
                     QGQWSQAVHYLQKVVRIDQKYQLPKLNENTQRLRALESQLSARTGTPHE"
     gene            9932..10282
                     /locus_tag="VLA60_18100"
     CDS             9932..10282
                     /locus_tag="VLA60_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HSF11329.1"
                     /translation="MEALTLPLPDEDWLLEITADGKLVCMAGYDLEDMKSMLSDGTPE
                     DLGTDELAKQAKFYLQQTVSKYRPKLMRLGFTERIEMNEAHVAAYYERPVDFGNLADL
                     HQQIKTCLTWFSSR"
     gene            10335..11360
                     /locus_tag="VLA60_18105"
     CDS             10335..11360
                     /locus_tag="VLA60_18105"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HSF11330.1"
                     /translation="MKPIPISIKSFKQFRDAIYAFRLPRIIFSALDLNLFTKMEDRDW
                     TIPELAKRVRVSQRGLAILCRNLASVGLLVKSQSGYRLAPFSKRYLQESSTDFQGDYL
                     VLMQRQWSEWSHLTEVIRVGQPLDSQEPETTEYRRSFSWAMHHRSIQPAREVAQQISL
                     KDDRTLLDLGGGPGTYALAFLAQNPTLHATVMDRSAALDVARKLAEQSSLGTRLTYQA
                     GDFLTARISGTYDVVWYSNVLHIYSPTDNLKIFKKIKRILNPGGRLLIQDTFLQDPNE
                     LLPLEANLFAVSMLLYTQRGNTYSVRDVRAWLQKAGLTRSGVLHLKKGTGDWEGQLIE
                     GRLPRSE"
     gene            complement(11506..>11699)
                     /locus_tag="VLA60_18110"
     CDS             complement(11506..>11699)
                     /locus_tag="VLA60_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HSF11331.1"
                     /translation="HEGTGLGLAIAHQIIQEHRGNIEVQSVRGGGTTFLVNLPSSPAP
                     PSVSPTQSPSPKAEVPNLD"
BASE COUNT         2982 a         2711 c         2924 g         3082 t
ORIGIN      
        1 gaagccatgg aacagatggg gcatgtccct tatggcgtgg cagcccgcat tcgaaaaaaa
       61 gtcacgatta atcctgtacg aatcgcggct atcgaacaag ttacaaagca tgatgtgatt
      121 gcctttttgg aatcggtatc cgagcaggca gggaaagacg gtcgatttct tcatgttggg
      181 ctcacctcct cggacattgt cgacacatcc ctttcgttgc aattagcgga agcagcccaa
      241 ttgatccgtg aggatgtcgg ggaattgctc aatgtcttac gggatttagc ctttgcgcat
      301 cagaatactc tgatggtggg gcgttcgcat gggatccatg gggagcctat ttccttcgca
      361 tggaaggtgg ccatttggta tcaggagttt caaaggcagg aactccgcct aaaagccgct
      421 attgccgaca ttgccgttgg caagctgtca ggggccatgg gaacatttgc ccatctttct
      481 ccctcgatag aaaaaacggt atgtcaacgc ttaggattga agccggcgcc catttcaaat
      541 caggtgcttc agcgggaccg gcacgcggcc ttcctctcgg cgttggccct gatggcagcc
      601 agtattgaaa aggtggctac ggaaattcgt catttgcaac gaaccgaagt cttggaagcg
      661 gaagagtact ttgagcctgg ccaaaaaggc tcgtctgcca tgcctcataa acgaaacccg
      721 atcagttcgg aaaatttatg cggtcttgct cgagtggttc gcagtaacag tctggctgcc
      781 ctggaaaatg tcgctttgtg gcatgaacga gacattagcc attcatctgc ggagcggatc
      841 atccttcccg atagcacgat tctgattgat tacatgttgg tccgtttaac gaacatgtta
      901 tccaaactcg tggtctatcc cgaacgaatg atggccactc tcaatcaaac cggtggcctc
      961 atctattcgc aacgacttct cttggcgctg gtggacaaag gtgcgatcag aaaagacgcc
     1021 tatgagcatg tacagcaaca tgccatggct gcctggaggg gggaggggag tttccaagcc
     1081 cttattgagc aggatccctt tattgccaaa catctcagta caaaagagat tgcgacatgt
     1141 tttaatccca aaacctactt acgccatcaa caggagattt tcacgcgagt gtttgggagg
     1201 caagttcgac gaaagagctc aacaccccgg gtcactcctt caaagaaaac tttaaaaaga
     1261 ggcaaataca ttgactaaag gcactatgct ttacgaagga aaagccaaaa agattttcct
     1321 gaccgaaaag gacgacgaac tcattcagta cttcaaggac gatgccactg cctttaacgc
     1381 cgaaaagcgt gggacgattc tggaaaaagg cattgtcaat aatgcgatct ccgcaaaact
     1441 gttccaacat cttgccgacg ccggaatttc cagtcatttc attcagcaaa tcaatgaccg
     1501 ggagatgtta acgcgtcggg taaaaattat tccggttgaa gtggtggtgc gcaatcgagc
     1561 caccggcagc attgtcaaac gtttaggttt agaagaaggc ttgattattg atcctccctt
     1621 agtagagttt ttctataaga atgattcact gggtgaccct cttatctccg aagaccatat
     1681 tcgattgttg aaattagcca cacccgggca aatcgaggaa ctccgacagc aggctctcaa
     1741 aatcaacgaa gtgctcaaac cttttttcca gaaaaagggc atgttgctgg tggattttaa
     1801 gttagaattt ggattatgga atggtcagat tattttggca gatgaaattt ctccggacac
     1861 atgccgtttt tgggatatcc aaacgggaga acgcatggat aaggatcgtt tccgaaaaga
     1921 tttggggcgg attgaggaaa cctatcaaga agttctcaag cgggtttgcg gataatgcac
     1981 gatgtagcga gccttgtgtt cgtgctttgc caccgactat cttgctgacg gatctgacta
     2041 tttaataaaa cgcttattca ccgggttcat gtcactcctt cccgaaaatt atgaaagcca
     2101 aaatttacat cacacttaaa aacggaattc ttgatcctca aggtcgagcg attcagcaat
     2161 cgcttcaaac ccttggtttt ggatcggttg gagaggtcag aattggaaaa tttatcgagg
     2221 tggatctttg tgaaacagac gcgacgtctg gggaacagac catcaaagcg atgtgcgaaa
     2281 aacttcttgc caatacggtc attgaagaat atcaatacga gatccttgag gcataatggt
     2341 ctctccattg ttccccctga ttaaattcga tcctttctct cctgagagct cttatctcag
     2401 tatgaatgtc aacgagcaga gaacgatatg aacatcggca tcattgtctt tcccggatcc
     2461 aattgtgatc gtgattgtgc gcatgttctt tcggacatta tgggacaaac agctgagttt
     2521 atctggcatc aagagagatc cgttaaaggc atggatgcca tcattctgcc aggggggttt
     2581 tcttatggtg actatctgcg cacgggcgcc atagccagat tttctcccat catggaggcc
     2641 gtcgttgagt ttgccgggcg aggaggaatc gtcctaggca tctgtaatgg attccaaatg
     2701 cttttagagc ttggaatatt acccggtgcc atgttgcgca acagggatct ttcctttatt
     2761 tgtgaagatc actatatacg cgtagaaaat gcgaatactc catttaccaa tcactgtaaa
     2821 cccggacaga ttctgaagct tccgattgcc catgctgaag gaaattatta tacaaaccct
     2881 gtgacgttgt ccgccttgca ggccaatgcg caaatcgtct ttcgatattg tgatcgcgaa
     2941 ggcaaagtga cttctcaaac caatcccaat ggatcgctgg ataatattgc gggaatccgc
     3001 aacgcggccg gcaacgtcct tggtctgatg cctcatccag aacgagccgc agaacacctg
     3061 ctcaataatg aggatggact atatattttt cgatccatgg tttcagcgtt ggaaaaacac
     3121 aaaacacctc agccggtata ggttcggttg ccattttccg cccctctctt gtcggtcaag
     3181 gccaccatgg aaatttttcc aggaattacg atgactccag gcatctgcca cgggaaacca
     3241 tgtgtggcag gcactgccgt tgatgtggct accgtggtcg gaacccttgg gatcggaaaa
     3301 tcatttcatg aggttcagga agcattgaat ctgacatatg aacaagtgct cacggcgttg
     3361 cgctatgctt cgtatgtcac ggaccatttg ccccttcgcc tcccttcagg tcgttaaccc
     3421 acaggacagt atgacggtgt ttatgctaag ttccctttta tggaaataag gactctcctg
     3481 actttccaat gtgcatttct gaagcattaa ggaaaccatc ctcgtgtggt tgtttcgaaa
     3541 ttattagtga gctatttggt ttttcacctc tttcattccc tttttgctaa ttgggaaaga
     3601 ttttcataca ccatgagttg tttgccatga cccaggaatc aaaagtcttg ccgcaggtac
     3661 ctcttacgtc aaaaattatt gaacagcatg gtctgtccga ggatgagtat cagcaaatcc
     3721 tcaagcatct tggtcgcgag ccgaatctca ctgagttggg tatcttttct gtgatgtgga
     3781 gtgaacactg cagttataaa agttcacgca ttcatttaaa acgatttccg accgagggtg
     3841 agcgggtggt tcaagggccg ggagaaaatg ccggtgccgt ggatattgga gagggcctcg
     3901 cggcagtgtt caagatggaa tcgcacaatc acccgtcata tattgagccg tttcaagggg
     3961 cggcaacagg tgtcggtggg attctgcgcg atatttttac catgggagca cgcccgatcg
     4021 cgttattgaa ttccctgcga ttcggtgaat tggaccttcc caagaatcag catattctca
     4081 aaggagtcgt ttctggaatt tcttcctatg ggaactgcat gggagtgcca acggtaggtg
     4141 gggaaattac gtttaacgat atttattcaa aaaatccatt ggttaatgtt ttttgtctgg
     4201 gtattgtgaa aaaagatcgt ctcctgcgtg gcttagctaa aggtgccgga aatcaagtgc
     4261 tgtatattgg tgccaaaaca ggacgagacg ggattcatgg cgccaccatg gcctctgaca
     4321 gttttgacga tgcctctgag aagaagcgtc cgaatgtaca agttggtgat ccctttttag
     4381 agaagttatt acttgaagcg tgccttgaat ttttagaaca ggacctctta gttgggattc
     4441 aggacatggg ggcggccggg ctgaccagct cctcctgcga aatggcctca cgcgccggaa
     4501 ccggtattga catggatttg gctaaggtgc cccgccgtga acagcacatg actccttatg
     4561 aaattttact atcggaatcc caggaaagaa tgcttctggt ggctcaacct ggcaaggaag
     4621 agtctgtgct ggcaatctgt cgaaaatggg gaattgatgc cgccgtggtc gggcaggtga
     4681 ccgatgatgg attcttacga attagagagg gcaaccagat tgtggcggaa atacccgcgc
     4741 atgccctcgc cgaagaaggt ccacgctatg aacggcctgc tgcgccacca ccttatcaag
     4801 agtttttgca atccttaaat attgatacgc tgccagatgt caaaaatcct gccgaggtgc
     4861 tcttgaaatt attagcgtca cccactattg cgagcaaacg ttgggtcttt cggcaatacg
     4921 atcatatggt gggaaccaat accgttgtct gtcccggttc cgactccgcc gtggtccgca
     4981 ttaaagggac cacgaaagcc ttggccatga ccacggatgg caatagtcga tactgcctgc
     5041 tgaatcccta tttgggagga ggattggccg tggctgaagc cgcgagaaat ttggtttgtg
     5101 cgggtgccca accgatcgga gtgactgact gcctaaattt cggcaacccg gagcgtcccg
     5161 acatcatgtg gcaattcatc atggcggtcg aagggatcgc cgatgcctgt cgaacattcc
     5221 aaattcctgt agtcagtgga aacgtgagct tttataacga gacgaatggc ctgtccattt
     5281 acccaacacc aattttggga atggtgggac ttattgatcg cccggaagac attaccactc
     5341 aatggttcaa acaatcaggc gatcatattt tcttgttggg ggagacgaaa gaggacttag
     5401 gcgggacaga atatctcaag gtcattcatt ctcgcgaaca aggcaatccc ccttggttgg
     5461 atctggatcg agaaaaagct ctctattcct tcctactcac cttaatccaa aaggggcttg
     5521 tgcaatcagc gcatgattgc tcagaaggcg gattactcgt gacattagcc gaatgctgtc
     5581 tgactcatcc ttccgcgcta ttcggggcaa ctgttatact gaaccaagat cgtttgcgtc
     5641 tagatgctct actttttgga gaaagtcctt cacgggttgt cgtctcggtt aaatcggagc
     5701 acgtcaagca gttgatggaa ctggttcaag gtacagacgt gccatgcacc gaattaggcc
     5761 aagtcactca aggaaatatg gatattaatg ttcgcggagc tcaagaccgg cccgtatgtg
     5821 agataagtct gcccctttct gaaatggaag accaatggca ccatgctctt gcacgacaac
     5881 ttggagccga agcatcgtga gcaaagttgg ttactcccta tttttgaatt ggtcagcttt
     5941 tatggtcgct caagatgatc tggcctgttt gaggacatca tgaggaagtt accgatggta
     6001 cagtctatgc cttctccgga cgggtttcat gaagaatgtg ctgtcttcgg aattgcggga
     6061 tcgaaagaag ctgcgaatct gacgtatctg ggcctctatg ccctacaaca tcgggggcag
     6121 gaaggctccg gtattgtgtc ctcggacgga gagcaattct ttcaaaaaaa aggcctaggt
     6181 ctcgttgctg atatttattc aaaaaaaacc ttacgcgaac ttcctggaac aaaggcgatc
     6241 gggcataacc gatactctac ggcagggagt ggacagttgc aaaacgttca acccttgtcc
     6301 gtcaatttcg cgtttggcaa tttggcgttg gctcacaatg gaaatttagt gaatgccggg
     6361 gtgcttcgag gtgaactcga ggcctatggg gctatttttc aatcggactc cgatagcgaa
     6421 gtcatcattc acctgattgc tcactccaag ggagatacga ttgtgaatcg ggtgattgac
     6481 gcattaagtc tggttcgagg ggctttttca ttggtgctgt taaccgatga tcaattaatc
     6541 gccgcccgcg atccgtttgg atttcgcccg ctgtgtttag gccggtataa aagcagctgg
     6601 gtcgtggcat ccgagacgtg cgccttcgat ctgattgatg ccaagttcgt ccgtgaagtt
     6661 gaaccgggag aaattattgt cattcagggt accgaattga cctcctatca tccgtttctc
     6721 gaacgctcgc gcgcccagtg tgtatttgaa tacgtctatt ttgcgagacc ggattcgaaa
     6781 atttttggcc caaatgccgt gtatccggtt cgtaaagcct ttgggcgtca actggctaag
     6841 gagtgtccag cggacgcaga catggtcatt cctgttccgg attccggtgt tcctgcggcc
     6901 ttgggctatg ccgaagggat ggggttaccg tttgaaatcg gactcacccg aaatcactac
     6961 gtgggacgga ccttcattga gccgcaacaa gccattcgcc attttggcgt aaagttgaaa
     7021 ctgaatccgg tcccggatgt cctggaaggg aaacggattg tggttgtgga tgactccctt
     7081 gttcgaggca ccaccagtcg gaaaattgtc aaaatgcttc gacaggccgg tgctcgggaa
     7141 attcatctgc gtatcagttc tccaccggtc attgccccat gttattatgg cattgatacg
     7201 ccgacacaaa aagaactcat agggtcaaat ctcaacattg aagaaattcg tcgttatgtc
     7261 acggccgata gtttggggta ccttagccta gaaggtatgc tggcaatggc tcccggcttt
     7321 tcccatcatt attgcaatgc ctgttttacg gataattatc ccatcatgtt gaccaaagcc
     7381 gagcaactgc aacttgggtt attcgaaccg gaccaactct ctcgctctta aatgtatttc
     7441 cgttttgttg ccgccccaaa aattcgtgtg gtaggctttc aagggatgtg aaggttgaat
     7501 ctctggttat atgccctagg aggcaccatg aacaggagga gcctatgaaa aggaatattc
     7561 ttcagttatt gatcgtagtc attttaatac tgagcaccgg ttttatcgca caggcgggaa
     7621 gtttctttaa agtcggggag tccgctccta atttctcact gacaggtctg gatggacgac
     7681 ctctctccct tgaccagttt aaggggaagg tggtggttct ggggctcttt catatttgcg
     7741 agccgtgttt aatccaaagc accaatctcc agagggtaca tgaagccaca aaagacaagc
     7801 ctgtcgcggt cattggagtc aattcatctg gcgactccct gtcagatgtc agggaattcc
     7861 tacaaggatt cccgttgaag attacgtatc cctatcttct ggaccccgat aagaaaacag
     7921 acaaactcta tggcgggggg aaatttattc ccaatgtgta tgtcattgat caaaatggcc
     7981 taattcgttg gcagcgtgta gggaatttct ttactgacgg cacattagcc ggacatgaag
     8041 cgattcttca ggaagtagag aaattgttgg aggcaccgcc tactccctcg accatgtagg
     8101 gcgatctctc ctgtttacac attcttgacc cgaatgttag ggcactatgg acgaactaga
     8161 agacgggaaa caaaaatttc ttgagatcgt gaaatccctt gatgcggcgg ttgaggtcgt
     8221 cattccgacc actccttcgc gcagtctgtt actcatttcc cttactaagg gccccaatcg
     8281 taaatttatt acggtccatg aggatgacat gttggatctt ccagaagatt taggcgttca
     8341 agccaaaacc acagcccttc tccaagaaac cctggaaacc ttatgaaaac cattctgccc
     8401 aatatttctg aatggtcctg gttctctgag gaaaaacagc ttaattttaa cggtcattta
     8461 ttggccgtgg gggaacatcg aatattagtc gacccgcctc ccatgaatgc cagtgatagg
     8521 gccataacgc agcggggagg ggctctagat tatattatct tgaccaatcg agaccatgaa
     8581 cgggaagcgg ccaatttccg gaaaatcttt aactgtcagg tgatggcgcc ggaattagat
     8641 gccaaggaga tgacccttcc cattgataaa acctttaaag atggggaatt gcttccaggg
     8701 ggaatttggg ttgttcagct ttcgcatcaa aaatctccag gagaaagtgc gttatttttg
     8761 cagcagggtc aaggggtact tattgtggga gatgggataa ttggagatcc tccaggttca
     8821 ctccggcttc tgccttcgga aaaatatatt gatatcgttc aagcccgcga tagtcttcgt
     8881 cgattattaa agtatacctt tgatagcctc ctggttggag atggaacatc cctgctcacc
     8941 ggagccaagc ctgcacttga acaattattg gcaaatgaat gaatcgaaac cctcataatg
     9001 gcctggaagg tttttcatcc gcactgtgac cacacgtgag cacccatggc ctggtctcaa
     9061 tccgccaatc tcaactcttt aggcaatcgc cacttttcca aaggctttta tacagaggct
     9121 tttcaatgtt atgcgcaggc tctggaagtt gatcgtcaaa atggagacca acggtcgttg
     9181 gccaccacat tgggcaattt gggtaatatt tgcgccgtga gtggtcgccg ggaacaagcc
     9241 agagcctatt atagtgaggt gttagagctt caaaaaattc tgggtgatga acgggggatt
     9301 agtacgactt tagccaattt ggggaacctt tcggccgatg ccggcgaatg ggatcgtgcc
     9361 cgggcgtatt atctcgaagc tttggattta atgaatcatt tgtccgacta tgccggaaag
     9421 gcggttttac tttctgattt gggattagtc gcgagggaaa cgggcaagga agaggatgcg
     9481 ctcgcctttt accaggaatc cttgatctta atgcgtcggg tcgggaatga ggccggacaa
     9541 tccgatgtct ttcgtatgat ggcacggtta tatttaagtc aacgacgatt tgaggaagcg
     9601 caatcctgta ccttgaccag cctggatgtt gccagacggt tgaaagatga actgcgaatg
     9661 ggcggtgcct ggtatgtcct tgccagttgc catgaggccc aaggtcaatg gagccaagcc
     9721 gtgcattact tacaaaaagt tgtacgcata gaccaaaaat atcaactgcc aaaactcaac
     9781 gaaaataccc agcgactacg ggctttagaa tcccagctga gcgccagaac tggaacaccc
     9841 catgagtgag tcctgcaccc cttcaacttt atggcctgag gccagaattt atcttgtgga
     9901 tgaaattcac gggtggatgg atatcgattc tatggaagcc ttaacccttc ccttaccgga
     9961 cgaggactgg ttgcttgaaa tcaccgccga cggcaagttg gtatgcatgg cgggatacga
    10021 tctggaggat atgaaaagca tgctgtcgga tgggacgcca gaagatttag gaaccgacga
    10081 acttgccaaa caagccaaat tttacctgca acaaactgtt tccaaatacc ggccaaaact
    10141 catgcgtctc ggtttcaccg aaagaattga gatgaatgag gcgcatgttg ccgcttacta
    10201 tgaacgcccg gtcgacttcg gaaaccttgc tgacctccat caacaaatca agacctgcct
    10261 gacatggttc tcatctcgct aactacatat tcgattccca catcgaggta ctcactcaga
    10321 ttagtcaaat tattgtgaag ccgatcccaa tttccattaa atccttcaag caatttcgag
    10381 acgccatcta tgcctttcga ttgccaagaa ttattttttc agcattagat ctgaatttat
    10441 ttacgaagat ggaagatcgg gattggacga ttccagagtt agccaaacga gtacgggtga
    10501 gccaacgtgg actggcaatc ttgtgccgga atcttgccag tgtaggttta ttagtcaaat
    10561 cacaatcagg ataccgtctt gctccgtttt ccaaacgcta cctccaggaa tccagcacgg
    10621 actttcaagg agactatctg gtgctcatgc agcgtcaatg gagcgagtgg tctcatttaa
    10681 cggaagtgat tcgtgttggt cagccactgg acagccagga accggaaacc acggaatatc
    10741 gacgatcgtt ttcctgggcc atgcatcacc ggtcgatcca accggccagg gaggttgccc
    10801 aacagatttc tctgaaggac gatcgcaccc tactggattt aggcggggga cctggcacct
    10861 atgctttggc cttcttagcg cagaatccaa cacttcacgc tacagtgatg gataggtctg
    10921 ccgctctgga tgtcgcgcgt aagctggcgg aacagtcttc tttgggaact cgcttgacct
    10981 atcaagctgg tgatttttta acagcacgca tctcaggcac ctatgatgtt gtctggtatt
    11041 ccaatgtgct ccatatctat tctcctacag acaatctgaa aattttcaaa aaaattaaac
    11101 gaattctcaa tcctggtgga cggctgctca tccaagatac ctttttgcag gatccaaacg
    11161 aacttctgcc ccttgaagcc aatctctttg ccgtctccat gttgctctat acccagaggg
    11221 gcaacaccta ttctgtgcga gatgttcgag cgtggcttca aaaagctgga ctcactcgtt
    11281 ccggggttct tcatctgaaa aaaggaaccg gagattggga ggggcaattg attgaaggac
    11341 gccttccgcg ttctgaatga ccgttctgta tggcttttca aaccccaatt ccctgtagcc
    11401 cataaagtct gaatatcttc attcagaacc cttctctttt gtggtgtttt aagggaacac
    11461 gcttggtgtg aatactacag agaccgaacc gaacagcggg gtgtttcaat caagattggg
    11521 gacttcagct tttggcgacg gactctgggt tggagaaacg gatgggggtg ccggagaact
    11581 cgggagattc acaagaaatg tggttccccc tcctctcaca ctttggacct caatgtttcc
    11641 ccgatgttct tgaatgattt gatgggcaat agccaatccc aagcctgttc cctcatgtt
//
